LOCUS       DASLOE010000001        75134 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.001, whole genome shotgun sequence.
ACCESSION   DASLOE010000001 DASLOE010000000
VERSION     DASLOE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 75134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75134
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.001"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..385
                     /locus_tag="U6309_000001"
     CDS             <1..385
                     /locus_tag="U6309_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN5503473.1"
                     /translation="SWLGLCPGNKVSGGKHLSGKTKPSNNKAAATLRMAASTLYRSQT
                     SLGAYLRRLKSRIGPMKAITATAHKLAIIIYNMLRHGVDYIESGQAYYDEKYRERMIK
                     NLKRKATEFGMSLIENQINFAPNIN"
     gene            complement(488..865)
                     /locus_tag="U6309_000002"
     CDS             complement(488..865)
                     /locus_tag="U6309_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEN5503474.1"
                     /translation="MTTPQITLSRVYSPPEHKEGLWILVDRLWPRGIKKESFPLDMWL
                     KDIAPSPALRKWFHQDSDHWHEFAKRYAQELKKKPELIEHVLALASTSPVTLFYAAKD
                     LQHNHALVLQKVLKSWPKIPEIK"
     gene            complement(1168..1920)
                     /locus_tag="U6309_000003"
     CDS             complement(1168..1920)
                     /locus_tag="U6309_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503475.1"
                     /translation="MLVTPRFKIMVVAKKREETQKDERARAIENNPCSICRSLGLPFC
                     RGHGGGSGGGGSDSASDDKVDNNDHQLLAPKNPANVTNIDQLLLHSGLWHLPEDEDFV
                     FEFKDPLALVNMTLNMELGLLVLQGKRELGNEEQNALDELYDTIAQEFNLFKNSIKDP
                     QVPLEAMTLAREGNSLTIKIPSPKYYDLFVQQLMEKNLLPGKAPVLQHQNQSVKHPEK
                     SEAAKDSVMEEYKSSLPNPFDISRGPKLPDWI"
     gene            complement(2016..2270)
                     /locus_tag="U6309_000004"
     CDS             complement(2016..2270)
                     /locus_tag="U6309_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503476.1"
                     /translation="MIELEPEPDTVLDPKDILTEEMKLEGSDNDMDHNQFTRGDEVDL
                     VGDGVPGYETTGQDGTDDEIEEQREARYTPTLNPNPKNVP"
     gene            complement(2513..2926)
                     /locus_tag="U6309_000005"
     CDS             complement(2513..2926)
                     /locus_tag="U6309_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503477.1"
                     /translation="MDIRCKTVLYKQLQEFNMINMKLKATLIACLSVLTLSSYANSSE
                     NKEAILQQCHDLASTVASLVSSQAKKTCAEKLVIASIHIDTAADWIVEDVHSAAKQEL
                     DNAIYSLQYAELNSCNRYIQISHSKLEAQRIKSLL"
     gene            complement(3121..4278)
                     /locus_tag="U6309_000006"
     CDS             complement(3121..4278)
                     /locus_tag="U6309_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEN5503478.1"
                     /translation="MDDLLFLDEQLHDDERMIRDSVSRFVSNDVIPLMAESYEHAHFP
                     KELIKKSADLGLLGLTLPAEYGGAEASYVAYGLVCQELERGDSGLRSFVSVQSSLCMY
                     PIFKFGSEEQKKRYLPEMAAGNIIGCFGLTEPDSGSDPASMRTHAKKIEGGWILNGAK
                     MWITNAPIADIAIVWAKTEAGIRGFIVDTKSEGMSCPEIKLKMSLRASITGELVFDNV
                     FVPDENLLPKSEKGLGAALSCLSQARYGIAWGAMGAAMACFDIARDYLMERKQFAKPL
                     ASFQLIQKDLADMYTEIIKAQCLNLQIGRLKDQHRETPTMISLAKGNACREALKIARK
                     CRNLMGGNGISLEYHVIRHMLNLESVFTYEGTDNVHTLVLGRHITGINAFG"
     gene            complement(4256..6493)
                     /locus_tag="U6309_000007"
     CDS             complement(4256..6493)
                     /locus_tag="U6309_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5503479.1"
                     /translation="MLDRASVVDEQFLKRVLQGDFPQLRSSMSPDTAELDKKTAIEFF
                     DSQIKSRLLDIIARQLKEKGLSFYTIGSSGHEGNAVLGQVFRASDMAFLHYRSGAFFL
                     QRAKQLPGIDGVKDILLSLVAAASDPISGGRHKVFGSVPLNIPPQTSTIASHLPKALG
                     AALSITRARELGLPSKLAPDSVILCSFGDASTNHATSQATLNACSWITQQNYPLPIVF
                     ICEDNGIGISVPTPSSWIKSSVSSRPGIHYIECDGLNIADVFAKAQEADYLARVKKQP
                     VFLHMRCVRLLGHAGSDIESQYNTQEEIEQREANDPLLHTAGILYREGWMSLQAMVEL
                     YQDNRALIEAKALEAIREPRMSCAEEIMASIVPRIDKKQKYSLPDDNRRAQVFAGAYN
                     QLALKRNLCQQINFALTDLMMQYPNMLIFGEDVGKKGGVYRVTADLQARFGQRRVFDT
                     LLDETTIIGTAIGLAHNGFIPVPEIQFLAYLHNAEDQLRGEASTLSFFSSGQYQNPMV
                     LRIASLAYQKGFGGHFHNDNSIAVLRDLPGVIVACPSNGPDAAKMLRTCIRLAYEEGR
                     VVVFLEPIALYMTKDLYSPGDNGWLFEYPSPDEMISQGEVGVYGEGDTVILTYANGYY
                     LSRQAEKVLREAHNISVKIIDLRWLSPLPKDAILKEIAKAKRILIVDEGRQSGSISEG
                     LMTLLMEEASPRLKIKRITGKDCFIPLGTAWQYLLPSQESIIDAVIALQSDKREKESG
                     RLVVS"
     gene            6731..7897
                     /locus_tag="U6309_000008"
     CDS             6731..7897
                     /locus_tag="U6309_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEN5503480.1"
                     /translation="MSVEMSKTSSVRFPPGQEIKLAKPATELYVGQILKTVVVTTLSN
                     DQVLININGQNLNAKASHHFTPGELFEVKVIANHPETILEVQQKTPLSSLLQNALLQY
                     LPKQAPATNLIQTLTQLMAENNLSAPINQVMKTILGNIPVLTQLPNQLMQAISQSGVF
                     LESKLLKGKIPREIQSDFKGLCFKLLHSLGTDSKLPINLPNELESGFLQQAPLPLPGA
                     IPQPLTKDTILNLIGLSTEAVHHILREQVTQALARITTNQINYLSQENQEAYLIMLDF
                     PLKTPDGIDVIPIMIKQHKAEPMQLSKWSISFALNLSHLGSIQATVSIYAKNIDIKVN
                     TQQSSTLQLLNDYHKDLEETLYESGLHLREWKLQVGLEENHIDATNLRLLDIKI"
     gene            7894..8160
                     /locus_tag="U6309_000009"
     CDS             7894..8160
                     /locus_tag="U6309_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEN5503481.1"
                     /translation="MKRKKPVAVALRYDGNSPPKVTAKGEGLFAQQIINTAKKHGIPL
                     EQNPELTALLSQVRLNEEIPKALYIAVAQILAFLYYINGKHSKE"
     gene            8283..8462
                     /locus_tag="U6309_000010"
     CDS             8283..8462
                     /locus_tag="U6309_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503482.1"
                     /translation="MKWIMLIVVIITTAGCCCTTAEVVEYQQVTVAAPVVEPVILDVD
                     DPEPLDVTTTTIEFY"
     gene            8644..11382
                     /gene="sidF"
                     /locus_tag="U6309_000011"
     CDS             8644..11382
                     /gene="sidF"
                     /locus_tag="U6309_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidF"
                     /protein_id="HEN5503483.1"
                     /translation="MPRITENIETYVMHLLSDLEPVPSQNQHLAYGYPGERQVFKDPM
                     LDGKQVVVVNSQYDKHGKPVTGQPDVIQEANNYIDNLVAAAKSLIDKDKKGEKDLRKN
                     AIDEMAKTFKKSISETIPSDKAKADLFSSKKSSANKDFLEQLAKVEGSLQQFTQAVAK
                     ASGHKLKALDKEGHNIRSHNRDTLIHRFKAPNSKEGEEQFIMYIPCGRYTKRQQRLMG
                     KDESEVNRDHDTTSHKRSDLVLGNSSMARMIAGTRHSDGTVTIHHDSFSGPGARMPYS
                     DFKGADDYKKLAIKAVTLINQEEIIQTLAQRQIDRMTNEDLWNKIPEEYRPDELPPDA
                     EKARAQLIKLYVEHNPLSVTECYTQVVTAGQRVAAENQKEQFEYVRQMMDAFDGSKAK
                     ITIQTGSNTEVETEVGYQARMSSWGVNWFRQVGALNPLSDNNVTKNQNARFVNQMTGD
                     VISNLDKVVQNLGDYDKAGALHTLLKGPDVSDLNQQITGKENALKEVKGAYREALFSY
                     FEEYQKGEGKWDQAKLEQLKNQVDGYEKSIKKQESAIYELHNQIDALRKAYYTEHKGQ
                     INKALQELKEQISPVIQNKETDPETKSKLQHFYNSCAYLMQAQELYYENTWHHGKNNF
                     KLQTLMASLSCELDYANTKGCKSNNDRGQRLAQKIVGNALWTAMSEDGLYTGEFLDHR
                     RTHGKDVSKVEQLDRELTTIQALHHTANTGVSGGKFEIQDKANFADNGLFGKVANFAK
                     VKEMGPENAFTKNMGTKIKAGIGLGLLAAGGLVALTLLFPPAGLAVAALGIGVAAGAA
                     IVAGVTFAATYLFGTAIDAINNKIEEGKIKSIVREGMKAASENSPQHKTEQKDDLTVN
                     AKLHQQLGVTRESVLERQSTPKQEFEKPKDMTSKVDLTPAMEDDKRSESTPTPVNF"
     gene            11408..12139
                     /locus_tag="U6309_000012"
     CDS             11408..12139
                     /locus_tag="U6309_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEN5503484.1"
                     /translation="MKKISALIFLYLISTASFSLPKGFVYLHDVAPDIIEDIRYATHN
                     NFIGNPIPGYKRGVCIVTRQTAQQLKKAEEYIKSKGYTLKVYDCYRPQRAVDYFYKWS
                     RNAKDNRMKPEFYPREMKQDLFDRGYIALTSGHTRGSTLDLTLVKLTASQEKKPNHPL
                     TRCYDKKPDYLDDDSIDTGTRFDCLDVSANIDYSRLSKNQKANRKLLQKLMSRFGFRP
                     YYYEWWHFTLKNEPYPDTYFNFPVE"
     gene            12340..13434
                     /locus_tag="U6309_000013"
     CDS             12340..13434
                     /locus_tag="U6309_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEN5503485.1"
                     /translation="MRVSKLLFLTLALSGNLFAQDIKVVGTLNQTLKAPQNKTLHTKP
                     AIQQIKLLKMQLSNSAIKALARKTDITLKKPNSITTSTALPSKVELGMNNVPVLNQGS
                     FGTCVTFATTAAVDAALNKGDYISQLCQLQLGLYLEKNGHSPSGWDGSLGRIVLSQME
                     NFGIVSKEKQKNLGCGGLTEYPDNEQPTPDHAMSLEQFHEMSEDPETNPVIYTPILDV
                     YQAVLDRTDTNKTINDIKTALNQKDRVTFAVLLLDFDLGVMGAVGTNKTQFDTWVLTP
                     EIARDIYLRPFFGGHEMIITGYDDNAIAVDDNGREHKGLFTLRNSWGEQFGDKGNFYM
                     SYDYFKVLTIEAQRIRALADNESEEESMTA"
     gene            13655..15205
                     /locus_tag="U6309_000014"
     CDS             13655..15205
                     /locus_tag="U6309_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN5503486.1"
                     /translation="MAQKPTKITHLVVLGDSLSDRGTLNKRELLGFIPMSYLSGLRSK
                     SPKGRFTNGFLWGDYVGATTAEQFEIEHIRRKLRLHNNARDNADIGDELLINDKARRE
                     NENSFSLNEDNHILFKGERFARFYCEGGLTSHDYSCSFTFNLVLFFTRLILATLGGKR
                     TQLLADDKKYNISNLEKSETLVVEWSGANDLITANDKPSRAAAEKAVNDRIQNIEALI
                     HNGYRNFVLFNLPNLSLTPRFQRKNAEEQRNASDCTEYFNQQLQTKSLELIKKYKELG
                     IPVNLSVFDVNEQFKEVYSNPEQYGFDRDKLKSPYVESDLFKENQKNPVDQKEHISPA
                     KGYMFWDDVHPTATMHDWLAERFKEKYYDKVFRFEPPLQAKHRHKETADALAKTTDSM
                     IKTDKPIKKLPEDIVLMLNAIHSRAQLMCQSTDAQRREKGELLKHFIFEIKCQYGNLE
                     NIYGLISAFTLNPDHSKIIKTHQNPMIDFFRNKVTTRSEDDIAALQKAVATYLQPKES
                     NEHLVKLT"
     gene            complement(15222..16379)
                     /gene="legS1"
                     /locus_tag="U6309_000015"
     CDS             complement(15222..16379)
                     /gene="legS1"
                     /locus_tag="U6309_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegS1"
                     /protein_id="HEN5503487.1"
                     /translation="MNFSIRVILLLTCFVSLAFSAPKFLTISDIHYGAENIAKDGQDT
                     GKEFLDVTFKRFEELSQNVDFILHLGDLPTHSLFSTSKKEEYEQVVFHGLHEANPGQK
                     PMFYITGNNDSLLGNYQPFSSDNKSPLNLALDWDGACVHCDGLIIDDTHMRKDGYYSS
                     YVIPNNKEIILIALNSVQWTKTPVFLPKYPNQQRDAFVQLFWLEQQLKNHRAKQLLLA
                     MHVPPGTAYNGNRFWHDIYLDRFLTLLDKYHQSYDQITILFSHSHMEEFRKIKLSDGS
                     TIYAFSTPGISRAHHNNPGMKIFDLDKQMRVRNFTTYFTSDLYEWGNEHYQAMNIPDA
                     IFPNCQNKTIPQCLDTLSPEQVCNNLEAGLFYGVKSTRVPYQGCIKTYQIN"
     gene            16634..16792
                     /locus_tag="U6309_000016"
     CDS             16634..16792
                     /locus_tag="U6309_000016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503488.1"
                     /translation="MITREQVDTQGAVHAEEMHKHRLEALARNMKSHTRLRDGINASS
                     AQTVRDNN"
     gene            complement(17191..18984)
                     /gene="dacB"
                     /locus_tag="U6309_000017"
     CDS             complement(17191..18984)
                     /gene="dacB"
                     /locus_tag="U6309_000017"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEN5503489.1"
                     /translation="MKRTLTGAFMWAVWSLSSHAASMQFEVDKLINRLNPHVNLGIVV
                     TDLTSGETLYKRNANRLYIPASNMKLFSEAAALMALGPDYQFKNQLSTSANQLQQGVL
                     HGNLYLHLSGDPSFSREDLKTLLSSLKDWNITTIQGNVIIDSSLMSIPAYPPGWLTSD
                     LSYSYGAPIAPLMVDSNRLTITVNPGAKAGAPAIVEVDDGGGTINLNNQATTKASEKG
                     CGVGFYLDPENNLTVRGCVGLGQWAVQQRIAIKNPFVYAQGMIVSELAKSNIKLNGQV
                     LLGRAPAGTLLIATRYSKPISQLMADTLKPSDNLYADSLYLHAAAKIKGSPVDWKQAQ
                     PVIKNFLQQQTGIDLKDSNFTDGSGLSRYNLVTPAQTMALLKFLYQRFPLSYEYIAAL
                     PISGRDGTLQKRFKTPNQQGFVRAKTGTMTGMNSLSGYLYTANGHTLAFAMYINRLPG
                     KPAGPGRPLLDALCTYFLQQSPTSSRLARVLSPHSRIKFQFNPTQIELQRVHQAKWRR
                     LETAVRQVLRGQDVNVVYRGNELIVTDNQSNANSVWKALQSIGKKYSFAVALSSKVMP
                     VTPSGKPLLLWVQAPLSENKAERTWVIREAV"
     gene            complement(19076..19867)
                     /locus_tag="U6309_000018"
     CDS             complement(19076..19867)
                     /locus_tag="U6309_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEN5503490.1"
                     /translation="MQTRFTYLPVTSLQAGQYQPRQDFNVTELQELAQSISSQGLIEP
                     LVVRPIAKERYEIIAGERRWRAAKLAGLNEVPCLIGDYSDQQACALTLIENIQRQDLN
                     LIEEATGYKRLIEEFHYHQDEIAALVGKSRSHVANILRLLTLTDKVKQLVRDKVLSFG
                     HARVLVGLNPEQQEMFAHQVIGEEWSVRHLEQEIKSYKNKDTNSPKNPRKDRDIERLQ
                     TILAEQVGAPVQIVNDGEDGGWLKVKFFDNDTLAGLLERLGLRYD"
     gene            complement(19984..20487)
                     /gene="ceg33"
                     /locus_tag="U6309_000019"
     CDS             complement(19984..20487)
                     /gene="ceg33"
                     /locus_tag="U6309_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg33"
                     /protein_id="HEN5503491.1"
                     /translation="MAAQLDPSSEFAALVKQLQREPDNPGLKQAVVKRLPEMQLLAKT
                     NSLALFRLAQVYSPSSSQHKQMILQSAAQGCTNAMLSACEILLKSGAANDLITAAHYM
                     RLIQSSKDSYIIGLGKKLLEKYPDFAEELNSKSKEIPYQSTLRFFGVQSESNKENEEK
                     IINRPTV"
     gene            complement(20661..21410)
                     /locus_tag="U6309_000020"
     CDS             complement(20661..21410)
                     /locus_tag="U6309_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN5503492.1"
                     /translation="MIIDWPVIIVLFCLSIPGVLIVMHRLVYFLLADNTERLKKRFSR
                     FVVLQTLFMTFVMSFAGSILSSRTGLGDPLLEGLLQGKASLDSFQVILLPTFLYALLG
                     LVIFCILYYGLFGSILDEKSFKIMSELRSKIGLDGCVLYGGVVEEVIARWGLMNVVCF
                     FALLFTSQINSEIIIISIFLSGFIFAVGQLPAYIAAGCTPSRRFLYSFVLLSLSQSIL
                     FGFLFWQYGLTSAILAHMLFHLGWDRYEKLS"
     gene            complement(21407..22690)
                     /gene="rsmB"
                     /locus_tag="U6309_000021"
     CDS             complement(21407..22690)
                     /gene="rsmB"
                     /locus_tag="U6309_000021"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEN5503493.1"
                     /translation="MKVNDRFNALKILTALIESRKNLSYLMSPQEISPMTKEICYGFC
                     RHYFRLQSIADTLINKRPKDNEVWIVLLIGIYQLQYMNKPAYAVVKETVSLLGQVKKS
                     WAKGLVNAVLRNFCRRQDHILEKLAAEPVFIYGQPNWLLQRLQKDWPNDWQFIAEAND
                     RHPPMILRVNVLKNSVDEYLDLLKKSGIEAFKHPVAPDGITLAIPCDVMTLPGFAKGS
                     VSVQDAAPQLAAYLLSLKPGQRVLDACCAPGGKTCHILEKESGLASCVALDLDPQRLQ
                     RVRDNLNRLDLQATVVQGDVLESRSWWDGHPFDRILLDAPCSATGVIRRHSDIKLLRT
                     DEEISAISQVQSSMLNSLWPLLAPGGLMVYATCSIMSDENELQISRFVAEHPDCQVSD
                     GKWSWGRWTGHGQQILPGEHGMDGFFYSVLRKDEK"
     gene            complement(22687..23631)
                     /locus_tag="U6309_000022"
     CDS             complement(22687..23631)
                     /locus_tag="U6309_000022"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEN5503494.1"
                     /translation="MNGLTVVFAGTPEFGLPCLDALIQSRHHLKAVYTQPDRPAGRGR
                     KLQESPVKEWAINHQIPVYQPLNFKNQGAIDELSALKPDVMVVIAYGLILPKAVLEIP
                     RLGCINVHASLLPRWRGASPIQHAILHGDAESGVTIMQMDVGLDTGPMLCKATCPVTS
                     SDTAGSLHDKLAKMSVKPLLDVLEALASNSAQFELQNNELATYAGKINKEEARINWHQ
                     SAVEIDRKIRAFNPWPVAYTLAGELMLRIHQAKATDIMSTEMPGMVLNIDKNGMLVAT
                     NDNALLVEKIQFPGAKVISVRDWLNSGKTQLHTGLMLQ"
     gene            complement(23628..24140)
                     /gene="def"
                     /locus_tag="U6309_000023"
     CDS             complement(23628..24140)
                     /gene="def"
                     /locus_tag="U6309_000023"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN5503495.1"
                     /translation="MAIRKILYLPDERLRKIAKPVETFDESLQTLINDMFDTMYDARG
                     VGLAAPQIGVSLRLSVIDIVGDKKEQIVIVNPEIVSSHGEKEFEEGCLSVPGAYDTVV
                     RAEKVTVKALDRFGKPFEITGEGLLAECLQHEIDHMNGKLFVDMLSPLKRMMARRKLD
                     KFKRLQARKP"
     gene            24234..25271
                     /locus_tag="U6309_000024"
     CDS             24234..25271
                     /locus_tag="U6309_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN5503496.1"
                     /translation="MRYCLLIFCFIISSITHALSLRPDSPSRYVVQPGDTLWSISSRF
                     LNNPWEWKALWRANPQIQNPDRLYPGAVLALEYYQNIPYLRVLSNGTIKLSPNIRVTP
                     QEDAVPPIPLGDIKPFLDESLILDVNVLNRAPYVVALMSERMLGGQGDEIYVRGLHPS
                     KEMPQGGTIGYSIFRGGRNYFDPITHELLGYKAVLVGYGELVAGGEPATVLLTSINQG
                     IKINDKVLINNHPEFELYFEPETPARQVRGYIIEMPDNMPVGNTQEAVGGVIIINLGE
                     TSGIKPGDVLAIYGKERIVNDPQNHLRPITLPQERLGEAMVFRVFTKASYALIVRSTR
                     AIHLLDTVTNP"
     gene            25268..26353
                     /gene="dprA"
                     /locus_tag="U6309_000025"
     CDS             25268..26353
                     /gene="dprA"
                     /locus_tag="U6309_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEN5503497.1"
                     /translation="MNNLQPLLALNRMKRIGPRTVLKLQKRWPDLNLMFQLSSVDLEE
                     AGLPSWLAQTIKNFDPDFIKTDLNWLSSSENHHILTWDSPYYPALLKEIADPPFILYA
                     KGELSALTQPSLAIVGSRNASVTGNENARAFSKEISRHGVSIVSGLALGIDAHAHLGC
                     LEGGGKTIAVLGTGIDCIYPSRHLKLAEQITENGLLLSEFPLKSPPIAGHFPLRNRII
                     SGLSLCILVIEAAIKSGSLITARMALEQNRDVLAIPGSIHNPLARGCHYLLQQGAKLV
                     TSVADVLDELKIEHHQFTSNKPIFSLASGKENLVKFIGFETTTIDQIIGRSGYGMEQV
                     TSGLAELELKGAVMAVPGGYIRCEYER"
     gene            26343..26780
                     /locus_tag="U6309_000026"
     CDS             26343..26780
                     /locus_tag="U6309_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEN5503498.1"
                     /translation="MKDNLFEMLLNLFEKSLTQLQKSHKSNDQDSQSQLLEEDLLSAE
                     EQSLFIKSAQQKSTRIFTYDEQMKLTKASYQFLMRMKLWNILDIDQFESIINQLQFSE
                     SRIVTLQETKWTIRNALASSLNEEQLTFLDLVLYQTEDELTIH"
     gene            26860..29139
                     /gene="topA"
                     /locus_tag="U6309_000027"
     CDS             26860..29139
                     /gene="topA"
                     /locus_tag="U6309_000027"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEN5503499.1"
                     /translation="MSKHLVIVESPAKAKTIQKYLGNDYDVLASYGHVRDLPARKGSV
                     NPEKHFSMTYVPIEKNARHIDTIAKTLKKSDSLLLATDPDREGEAISWHIFELMKERN
                     LLKDKSVHRIFFNEITKAAIQDAINHPRSISMDLVNAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLIVEREEEIERFIAQEYWKIIAKCAHASTEFEARLTHYNEE
                     KLQQFSVTQQEQAHEIKKQLIAQAQGFLTVAQIDKKQRKRKPSPPFITSTLQQEAARK
                     LGFTARKTMMVAQQLYEGIDIGTGTVGLITYMRTDSVNLAKEAIDEIRDYITQRYKGD
                     NCPNSPRIYKTKSKNAQEAHEAIRPTSIKRTPEMVQGSLTSDQLKLYSLIWKRTVASQ
                     MADAILDTVSVDFSCGKGNTFRANGSTIAFPGFLSVYEEGRDDSKDEDNEDKILPAFN
                     VGEKIKVSDIETNQHFTEPPPRYSEATLVKALEEYDIGRPSTYASIIHTLQQREYVVV
                     EKKRFLPTDVGRIVNRFLTNYFTRYVDYQFTAGLEDTLDAIARGEKDWIPVLEEFWQP
                     FVQQIQNIDEQVQRKDVTTELLEEKCPKCQKPLSIRLGKRGRFIGCTGYPDCDYTQDI
                     SNPEGEKSEPEVVEGRSCPLCHGALHIKTGRYGKFIGCSNYPECKHMEPLEKPSDTGV
                     TCPKCSQAKILQRKSRKGKIFYSCGNYPKCDYALWNEPVDLPCPKCAWPILTLKESKK
                     FGRQILCPREGCDYSAKED"
     gene            complement(29241..31214)
                     /locus_tag="U6309_000028"
     CDS             complement(29241..31214)
                     /locus_tag="U6309_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN5503500.1"
                     /translation="MNYRSDIDGLRAIAILFVLFFHGGLTLFPSGFIGVDVFFVISGF
                     LITSIIEKSLQNNRFSFMDFYSRRLWRLQPVFICLIVVTTLVTLFYYLPDDLMLFSKS
                     ARKTSIFTSNVFFKNVTSDYFSPDSNQLPLLHMWSLSVEWQCYFILPVGIYLLYRLVG
                     PQHRIKITYLLTITFFALSMYYSWSNPAKTYYQFSSRIFEFLIGSCVASASGSKRFLA
                     NKYVLNFITVLAVLTLFYIAMHHDINIGFPNWYTLILCLATAILIAAGQQESQSIVTY
                     ALSLRPLVFIGLISYSLYIWHWPVLALMRYLNIEESNLVLLVTFGLILIIAYLSWRFI
                     EKPARKLHRIRFSYTVVCLLIMPVFVTHLSDYLIKKNAGYPHRFNEAIQVYAELNKYK
                     NPQRPLCLERKNTEVNPQCVLGAKNSDSRTGFMIGDSYSNQHWGFMDTLAKEANVSIL
                     AHATVACLALPDIYQFDWNVKNKIYQECHDQANRYFNMIKTNHYDYVILGQNWNGYFG
                     DRIINQLNDNRSQELSLKRIEKALDKALKLITTSGAKPVLIKSIALSKGNPYNCFFEH
                     IKLHKKYNPERCDFNLDVSEQIWFDNLFAKMQRKYKTLVIIDPRVAQCPSGMCKVDIN
                     GIPVFRDSGHITDYASYHLAKLYLQKNNNPLVS"
     gene            complement(31350..31790)
                     /locus_tag="U6309_000029"
     CDS             complement(31350..31790)
                     /locus_tag="U6309_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEN5503501.1"
                     /translation="MAENTTESNVIYFTRLHWIIFFGPFVGLGLVMAIYIYIIQLREV
                     SLFLGFFALIWILITWVTYHFSSITIKRKQVILRTGIIVRQTVDIPLNKIETIDIRQS
                     ILGSILSYGTLIITGTGGTRYLINYLYKPLTCRRYIEQLMNEPH"
     gene            31921..32343
                     /locus_tag="U6309_000030"
     CDS             31921..32343
                     /locus_tag="U6309_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEN5503502.1"
                     /translation="MKSISQCLNKQLSTICQHSMQLEELSLKISAFLPSHFISKCQVG
                     SFNKGCLVLTTTNAAFASEIRYLLPELRDKLRKEAGLYQLSSIKITIIAPSSQIENPA
                     QKKSLTLSEKAKATIISESQHCTYEPLKNALLHLAQSE"
     gene            32409..33665
                     /gene="lem28"
                     /locus_tag="U6309_000031"
     CDS             32409..33665
                     /gene="lem28"
                     /locus_tag="U6309_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem28"
                     /protein_id="HEN5503503.1"
                     /translation="MLELAMPKINKIVNGKDLTPHYLSEPNKEFKIYRYNKEVYAVRF
                     ENYEPIDYVLMWKSHKSEHTQNSEMIKNTEEDYKELGKGEQGAVYEKTEDKAMKVSRG
                     RHPREFYEEINLHIIEQQFFLKYHGIQEHFVLGLWSIKNEENVYFYMPKINAIPINKK
                     IDQPKIEEFVLALKELNDAGYWHPDLANNPYHISPQNLIATEEMVKTIDLDGGFRYDK
                     GRVDELSRKSLVYGKDQWLYVYNFIYPPTDEEDNRIDWRVPIEKWYENNRDESLSDNP
                     HALLRFYHEGLISLPKKLAHDLHETILEELSQDKKFKKHSHEVGVNRFFSPKKQEIYE
                     GLKGDGLKKVILKELRDSLAEIRTMEQLEEKKLEFLASPEMQILAEGQDKTTKALNLK
                     TSSRKKVMVIFKEAEERILNSPNVSI"
     gene            33965..34762
                     /locus_tag="U6309_000032"
     CDS             33965..34762
                     /locus_tag="U6309_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN5503504.1"
                     /translation="MLKQIVLTGLVIIGIVITLMYLFQRHLIYFPNRHTPKLEDYNAS
                     DMKVISLRTKDNLHLKSWYKPASKHRPTILYLHGNAGHIGYRMPLVREFIDAGLGVFL
                     LEYRGYGGNPGKPSEKGLYADGETAIEFLIQHGVSSKRVILYGESIGTGVATHLATKY
                     PVCAVILQSPFTSLTRLAQYHYPLNFLKPWDQYNSLARMKKINAPILVLHGKLDQIVP
                     YQEGLNVFNEANEPKKMISFDDKEHNDLWSADNFSREIIQFALNQCS"
     gene            complement(34817..35233)
                     /locus_tag="U6309_000033"
     CDS             complement(34817..35233)
                     /locus_tag="U6309_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503505.1"
                     /translation="MSFSLSELLHYKNEKIVTHFCYSHPEYSIQEGLFLFEDLLAWMW
                     LNKQRRLRGKKTYLFGPLLILDEMWHSFILHTRDYIDFSMKYFGEYFHHDVEPVGFEH
                     VLDEEELADYLQDCFQYLNKEWVERRFSMAFAEMDD"
     gene            complement(35348..36199)
                     /locus_tag="U6309_000034"
     CDS             complement(35348..36199)
                     /locus_tag="U6309_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEN5503506.1"
                     /translation="MCRFVAYLGRPTLLENVLVRPKNSIIMQSLHARETKYRTNGDGF
                     GLGWYSPEVSSDPALFTSVYPAWNDRNLLHLTAKIQSPCFFAHVRSASAGGVTNYNCH
                     PFIYGDWMLMHNGEINDFIDVKRHIRHLLDDDIYHWVKGQTDSEHLFGLFLQLAKGRD
                     INQLSVAADVLQETINKILEVIGQFGHSGPSYFNICLTNGKKIIATRYCSHEKKKPLT
                     LHYLLGYVLSTQGLWIKEEGNPAYVVVSSERLNDLDEGWEDVPPQHMILIDENKNIQL
                     RPLQTLG"
     gene            complement(36353..38389)
                     /locus_tag="U6309_000035"
     CDS             complement(36353..38389)
                     /locus_tag="U6309_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEN5503507.1"
                     /translation="MKFKIATCIVTMLSSFIVFSSNSNFSDNNTNDLLHLDWRDTSVS
                     PAVDFYTYANGNWKKNNPIPADYASWGSFYVVNEKVQNIIHQMLISASKNTHAKPGSI
                     EQKVGDFYFSGMDVDSINKSGITPLKPEFTKIEAIKNLKDLQAEIAHLHKIGVGPLFN
                     FGSMQDFKDSTSMIGAVMQGGLGLPDRDYYLKKEAKFKQVREAYVNHLTKMFELLGDS
                     PEMAAKEAKIVMDIETQLAKASMSQVEQRDPHAIYHMMTITELDKLTPNFSWSQYLSA
                     RGQGKIKKINFAMPDFFKAMNELLQSVSLDEWKIYLRWHLIDAFAPYLSKPFVDQNFK
                     MVSALTGTQKLLPRWKRVVNTENGALGFAIGELYVEKYFSPESKQKVLDILKNIRAVL
                     QEDIKTLSWMSPKTRDAALKKLDLMEERVGYPAKWWDYSSLKIDRGPYVLNVIRANEF
                     LINRDLDKIGKPVDRSEWAMTPQTINAYYDPSMNNLNIPAGILQSPFFDPKAPTAINY
                     GAIGYVIGHEMTHGFDDQGAQFDGYGNLKNWWAPNDLSKFKNATQCIADQFSKYVVDG
                     DLHVQGQLVVGEATADLGGMILAYKAFQRSKEYKNAKTIEGITPDQQFFLGTAHVWAM
                     NVRPEQLRNQVTTDPHPPAVYRVNGSLANIPQFQEAFNIPNGSPMISKNRCIIW"
     gene            complement(38534..39448)
                     /locus_tag="U6309_000036"
     CDS             complement(38534..39448)
                     /locus_tag="U6309_000036"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEN5503508.1"
                     /translation="MIKQRTPKKVIQATGVGLHSGEKVLLTLRPAPVNTGIVFRRVDL
                     SPVVEIPASYEYVGDTMLCTTLHHGKVKIATVEHLLSALAGLGIDNAYIDVNAPEIPI
                     MDGSAAPFVFLIQSAGIREQNAAKRYIRILKPIRVEENGKYVQFLPHKGYKITFTIGF
                     EHPVFNDRPQTVSFDFSGTSYVKEVCRARTFGFLSDYEKLRECDLAKGGSLDNAIVVD
                     DYRVLNEDGLRFESEFVTHKVLDAIGDLYLLGSSLIGAFEGYKSGHELNNRLLRELMV
                     RQDAWEYTYFDTENYLPAVHPEYYPVEA"
     gene            complement(39696..40892)
                     /gene="ftsZ"
                     /locus_tag="U6309_000037"
     CDS             complement(39696..40892)
                     /gene="ftsZ"
                     /locus_tag="U6309_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEN5503509.1"
                     /translation="MFELMESSQQENNAVIKVVGVGGGGGNAVEHMVAENIDGVEFIC
                     ANTDAQALRGSSAKIHIQLGDALTKGLGAGANPQIGREAAEEDREHIKEILSGADMVF
                     ITAGMGGGTGTGAAPVFAEIAKELGILTVAVVTKPFSFEGKQRALAAEEGIRRLAEHV
                     DSLITIPNNKLLSVLGKNISLLNAFKAANNVLLGAVKGISDLITRPGLINVDFADVRT
                     VMSEMGMAMMGTGSAVGEQRARQAAEAAIASPLLEDVNFSGARGILVNITAGLDMSIG
                     EFEEVGDVVKEFISDDATVVVGTVIDPEMTDEMRVTVIVTGLGDNRQRQQQPQQPLRA
                     RLVETTRSDGSLDYQQLERPAVIRKQAQTGTATTATKQTNNGNESVPDVDYLDIPAFL
                     RRQEEA"
     gene            complement(41087..42325)
                     /gene="ftsA"
                     /locus_tag="U6309_000038"
     CDS             complement(41087..42325)
                     /gene="ftsA"
                     /locus_tag="U6309_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEN5503510.1"
                     /translation="MAKKIEKNIITGLDIGTSKIIALIGEVTSDGTIEIIGIGRHPSR
                     GLKRGVVVDIETTVNSIQRAVQEAELMAGCEVRTVYAGIAGSHIRSLNSHGIVAIRDQ
                     EVSQADVERVIDAAKAVAIPADQKILHILPQEFIIDHQGSIREPIGMAGVRLESRVHI
                     VTGSVSAAQNIVKCVRRCGLDVIDIILEQLASSHAVLTEDEKELGVCLIDIGGGTTDI
                     AIFCEGAIQHTSVIPIAGDQVTNDIAMALRTPTKAAEAIKVNHACALPELANPNQMLE
                     VTSVNDRPGRKISAKALADVVSARYEELFSLVRNELRRSGFEDRLAAGIVLTGGASNV
                     RGAIELAELCFEMPVRKGCAHYVSGLTEATENPSFATGVGLLLHGFQQQYESGYNVPA
                     MNDNGKSIWSRMKEWFQGNF"
     gene            complement(42325..43044)
                     /locus_tag="U6309_000039"
     CDS             complement(42325..43044)
                     /locus_tag="U6309_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEN5503511.1"
                     /translation="MEVNSVGLRYLTIMGLLILSALLLAGRLGYLYLADAERFPITTI
                     KVAATYEHITHKELENVLAKYLDASFFLLSVKGLQSELNSMSWIDTAYVERVWPDTLK
                     IKLTEKKPVAIWGDALMTRDGKLFNQGSLPSDLDIPKLKGPQSQQLEVLQVYEKLSKI
                     LSSYGLNASGLYLRDNQSWVLLLNHSVKIYLGKKELEERLLRFCKAYPAVFAEKADQL
                     AGVDLRYPRGMAVQWKQQMGR"
     gene            complement(43060..44154)
                     /locus_tag="U6309_000040"
     CDS             complement(43060..44154)
                     /locus_tag="U6309_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEN5503512.1"
                     /translation="MSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAM
                     DKSGQFHRHDYNDLLACSDKLPVVTEKSTPLEGLLINGRLAVDAEIVFPVVHGPLYED
                     GCLQGLLELAGVAYVGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQ
                     QFCHEVASEFGWPLFVKPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVG
                     REIELAVLENSIPCGKPRVSMVGEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSL
                     EEQLKQAAANIFSYLKCKGMARVDFFVNDKTEEIYFNEINTLPGFTSISMYPKLWQAT
                     GVAYPDLLDELINLAMVHHNCRQHLVTNYL"
     gene            complement(44138..45064)
                     /gene="murB"
                     /locus_tag="U6309_000041"
     CDS             complement(44138..45064)
                     /gene="murB"
                     /locus_tag="U6309_000041"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEN5503513.1"
                     /translation="MSITGMDSGHVTESQGTLLFNEPLAEYTTWRVGGPAARLYKPAN
                     IDDLALFLSRLPFDEPLLWLGLGSNSLIRDGGFSGTVILTQGCLKEMTLLSDNCIRVE
                     AGVSCASMARFSARNNLSGGEFWAGIPGTMGGALRMNAGCHGGETWQSVIEVQTINRR
                     GEIRTRKPEEFEVAYRHVAGLGDEWFISAKLQLSPGNKETSLQLIKDLLAHRAKTQPT
                     NEYNCGSVFRNPPGDFAARLIESCGLKGVSIGGAVVSEKHANFIINHQGTATAANIEA
                     LIHLVQTKVREQTSIELIREVHIIGDANVQTR"
     gene            complement(45073..46482)
                     /locus_tag="U6309_000042"
     CDS             complement(45073..46482)
                     /locus_tag="U6309_000042"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEN5503514.1"
                     /translation="MNNSEQFLSPRMGRVEQIHFVGIGGAGMCGIAEVLHNQGYRITG
                     SDLGESGTVQRLRSLGIQVYIGHRLENIKGADVVVRSSAVDFNNPEIVAARELMIPVI
                     PRAAMLAELMRFRHGIAIAGTHGKTTTTSLVSSLLAEGGLDPSFVIGGKLNSCGANAQ
                     LGKSAYFVVEADESDASFLFLKPMMAVVTNIDADHMDTYEGDFEKLRTTFLEFLHHLP
                     FYGLAVVCLEDEEICRILPAIQRPTLTYGFKEEAHYRAINWTQKGMLSEFVVVRPAPH
                     KQLTIQFQYPGRHNVLNALASIAIATELGVDDDSIVRGLQKFQGVGRRFQMLGEKQFE
                     KGAAIIVDDYGHHPQEILSTIDAFRRVWPERRLVHVFQPHRYTRTQSLHRQFVDVLSL
                     SDELLLMDIYAAGETAIPGVTSENLANEIRSRDKRVTIVSEQSLKATLDEFIKDGDVI
                     LMQGAGSIGQMAVNLMKNM"
     gene            complement(46492..47667)
                     /gene="ftsW"
                     /locus_tag="U6309_000043"
     CDS             complement(46492..47667)
                     /gene="ftsW"
                     /locus_tag="U6309_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEN5503515.1"
                     /translation="MRPRHLNQRGKPVSRPISLYDKWLIGAVFGLLIIGLMMVASSSV
                     MISTKYFHQPFHFLIRQACYLFVGLLLALIVVRTDSSFWEKISMPMMIGCVFLLLIVL
                     IPGIGKSVNGSRRWLALGPIGVQVSELTKLAMIFYLSGYLVRQQEAVCESIFGFIKPM
                     AILAVVSVLLLLEPDFGATVVISGTVMAMLFLAGVKLRYYFGLMLVVVTALALLAVSS
                     PYRVARLTAFLDPWADQYNSGYQLTQSLIAFGRGGWFGTGLGESIQKLLYLPEAHTDF
                     LFAVIAEELGLFGILVVITLYSILVIRGLNIGYTAYTQERHFASYTAYGLTIWLALQA
                     SINMGVNAGLLPTKGLTLPLLSYGGASMVINCIVIALLLRIDHENRWQSLGLRPLTA"
     gene            complement(47673..49016)
                     /locus_tag="U6309_000044"
     CDS             complement(47673..49016)
                     /locus_tag="U6309_000044"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEN5503516.1"
                     /translation="MNHSLYLVAGLGKTGLSIARYLKKNNKSFVVFDTRKEAPGLAEF
                     QNEFPDVPIYLQQTPDEVISQVTDVITSPGLALDTPVLERARQAGASIYGDIECLARE
                     ISAPVIAITGTNGKSTVTTLVGEMAKAAGFRVAVAGNIGTPVLDMLDDEHHYDLWVLE
                     LSSFQLDLTYSLSPVVATILNVTPDHLDRHHTMEAYTQAKQRIYRGAKAVLFNREDVY
                     TVPHQSCQADIKCISFGKDAPSMGNWGLIEQENTTYLAKGMERLLPVESILIKGVHNW
                     MNALAACALAEAAGISMQHILNVLKTFPGLPHRCQWVREVDGVGWINDSKGTNIGATI
                     SAINGIGGSMQGKIVLIAGGQGKGADFQELAQPVSEFVRSIVLIGEDADKIESALAKV
                     VPVVRASSLEGAVTIAKTCAKPGDVVLLSPACASLDMFRDFNHRGDVFTSSVRGL"
     gene            complement(49030..50115)
                     /locus_tag="U6309_000045"
     CDS             complement(49030..50115)
                     /locus_tag="U6309_000045"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEN5503517.1"
                     /translation="MLYWLTQLLQGQYHAFRVFQYLTFRSILASLTALIVGLLCGPLM
                     IRWLRGLQIGQMVRSDGPQTHLSKAGTPTMGGVLILLAITVSCLLWCDLRQTSLWLVL
                     LVTLANGLVGWVDDYRKLVLKNSKGLPGRWKYFWQSVIALVAVSYLYWNASLPVHTQL
                     TVPFFKTVTWDLGVFFPVLAYFVIVGSSNAVNLTDGLDGLAIMPIVMVAGALGVFAYA
                     SSNAVYSNYLGIPYVPNTGELTIFCSSIVGAGLGFLWYNSYPAQVFMGDVGSLALGAA
                     LGIVAVVVRQELVLLIMGGLFVIETLSVILQVGYFKYSGGKRLFRMAPLHHHFELKGW
                     SEPKVIVRFWIITVVFVLCGLATLKLR"
     gene            complement(50230..51567)
                     /locus_tag="U6309_000046"
     CDS             complement(50230..51567)
                     /locus_tag="U6309_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEN5503518.1"
                     /translation="MNLITIASLLSQSCQINADITGVSIDTRTLKPGNLFFAIVGERF
                     DGHDFIKEAEAKGASAAVVNRSIDGVHIPQFVVVSPLMALAKIAAAHRQEMSCPIIAL
                     TGSNGKTTVKEMIATILPQPSHATKGNLNNHIGVPLSMLALNKQHRYAVFELGANHPG
                     EIAHTVGIVQPDVSLINNIAPAHVEGFGSIDGVARSKGEIHQGLSPTGVAIVNDDDAY
                     AHFWDDLIADKKILRFSSLHPADIYAQDIRLDTHGRAHFTLVLPNSRVEIELQVPGLH
                     NVRNALAAAACCYAVGISGNDIQRGLAHFGGVKGRMTMLSGQNQSTVIDDTYNANLRS
                     VLTALEVLSGRPGRKIFVFGDMGELGEWTTQHHREVGIAARQLGIDKLMSCGLHSELA
                     SQAFGEGGQHFCSQEELIEVVKNDLTPDTTVLVKGSRSSAMENVVHRLVQVEG"
     gene            complement(51808..52587)
                     /locus_tag="U6309_000047"
     CDS             complement(51808..52587)
                     /locus_tag="U6309_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEN5503519.1"
                     /translation="MQANWSLILNVLLLIGVLVAIGRLMKTRRQSLNQEHYQPSLGQV
                     ENNSYGSQSYNDEIIAVRKVNREVPLDFENFDGSEKRQAIRKPSVKTEQPQLIPEEEE
                     CESNERTEPNNDCSSSTAMVFLLAKENRQFAGYELLQTVLAAGLRFGERHLFHRHQFT
                     NGQGPVLCSLAAATATGVFDLQNIGAFSVRGLCLFMQISNNPEINAERFSVMLDTAKQ
                     LSEGLDAYLLDEQRKPLTEERIARYHRYLKIEHLMDDCVTN"
     gene            complement(52592..56086)
                     /gene="smc"
                     /locus_tag="U6309_000048"
     CDS             complement(52592..56086)
                     /gene="smc"
                     /locus_tag="U6309_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEN5503520.1"
                     /translation="MHLKQLKLAGFKSFVDPTTVHFPSRLVAVVGPNGCGKSNIIDAV
                     RWVMGESSAKNLRGESMTDIIFNGSSNRKPVGQASVELVFDNSLGRLTGPFASYGEIA
                     VKRVVTRDGESFYYLNGARCRRKDITDIFLGTGAGARSYSIIGQGTVSRLIEAKPEEL
                     RVYLEEAAGVSKYKERRRETLQRIEQTRDNLTRVADIREELGKHLQRLERQAKAAERY
                     TILKEEEKICRAEIQALKWQEYSEKQTVKQRELQELAIRNEQQQSLLTSCYKERAVLN
                     EQLQDAIDQSQSIQDSLYQSGTEIARLEEAIQQQEREKKRLENDRQQMQVDSQLIEEQ
                     LKLDNEELHNCKLKAQQLEEHLHHLKMEFKEQESLWQEKQKEQSQWEKIWQEVQINSN
                     TIKNEYQLTQAKLQHLEQKRHQVLVRLEKLNLEQSAISLDDLQKNKIKLQEKHFQLKE
                     TQEFDEEQLRQANETQSDLQNQLQNTEQQLHALQDEFHRLNTELAALKAAQIAAKKEN
                     RPNKDGIAEWSDKPRLMDVLKVEPQWQSSCEIVLGDDLIAYVLDDFAELWSRWQFCED
                     HKESVVTMRALNGEKQERPRLADKIQGLVPSTVNHLEHIYAADNLEEAKAWFPDLLAH
                     ESIVTVNGIWLGKGWIKLPRQKEQDEVGVLARQQRIVELNLSVESLQQKIESIRLARD
                     SYQARLQENLNFIKLHQLNLNASNEALRNHIIAVNNQEQAIFHAEKQKDAITNEIIEL
                     ESSLEELNAEQLEMSDKIRELEGQYQASELQFKRFINEKDTWNSVLVEQNKQVESVRA
                     LLHQSELEYDREVTKIQQITDRISREKERLQRAQMRLEELTQHYLQSVAPEAEFKNQL
                     AALLIKHGEIESMLTLSREKVSQLKLQLEDQEKLILNYDQEVKRIQELMNQIQMEKQA
                     LSVRASSIQETLDEANLQAQSLLERIPSGVTQSIKEDELIAILEKIKRLGAINLAAIE
                     EFAAEQQRKVFLDEQYNDLSQALSTLETAIEKMDNETKSRLENTFDEVNTSFKALFPR
                     LFGGGRAQLELTCDNLLEAGIVVMAQPPGKRNSSIHLLSGGEKAMTAVALVFAIFQLN
                     PSPFCMLDEVDAPLDDLNVSRFCDLVKEMSQFIQFLFITHNKVTMELAEHLVGVTMRE
                     PGVSRLVAVDVKQALTME"
     gene            56261..56941
                     /locus_tag="U6309_000049"
     CDS             56261..56941
                     /locus_tag="U6309_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEN5503521.1"
                     /translation="MKQQFYKLPQILISLCLLLILSTPIQAEPPNLSLIRKEIKNYYD
                     SGLYYHELERTIKLAQEYIHQQYLINKNNKHPKKLAIVLDIDETSLSNYDKMVKRDFT
                     GSKEQIHKEILAANSPAIKPMLTLYKNALKKGIKVFFVTGRQESERDATRANLIKAGY
                     TKWAGLYLRPNGYSSPSIIPFKSKAREMIAKKGYTIIASIGDQCSDIQGGYTKKGFKL
                     PNPFYYLP"
     gene            complement(57043..58104)
                     /locus_tag="U6309_000050"
     CDS             complement(57043..58104)
                     /locus_tag="U6309_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEN5503522.1"
                     /translation="MSKLINLAVLSFSSSLAFAGPLTLKGEVDVHITPKNPSGVAQSL
                     TFKLPKYELSTEAKSYLREQLSEYPKNSINTASFSSELPRKVKLGMQLTPVLDQGYHG
                     SCVTFAVTAAIDAALGAGDYISQLCNLELGSYLAIHDKAKASGWNGSFGYWVLQQISE
                     YGIISQNYQKLNGCAGVREYPLEDENNEGKPMSDSEFLAHSVPVSNLISWEALLKDEE
                     SFSAKADMNQIVYQIKEELAKGNRLTIGMLLDVFVGDAGAVGTNRAYNDTWMLTPEIV
                     LDAMNGMIYAGHELVITGYDDDLEVMDEEGHVNKGVFTLRNSWSKFAGDQGDYYVTYD
                     YVKFLAMEVMAIRMKEKAA"
     gene            58414..59226
                     /locus_tag="U6309_000051"
     CDS             58414..59226
                     /locus_tag="U6309_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HEN5503523.1"
                     /translation="MRTLLSCFLITLLSFGLIINEASAKRFGGGRSFGVQRSQSSLFS
                     SNKTYNKSSLTQKANKSRWGGVLGGLLIGGLLASLFMGNGLANGLITWLILGSVIFFV
                     VSFLRRKMNPGFQATQSGAFRQNSFEQFTQNYGNRNSGGAHFAQQETSFDADNFLRDA
                     KVTFIRLQAAYDQKNLQDLQTFTVPEVFAEIKMQLDERGDAPNQTEVISLDAKLLDVS
                     KQSLSTIASVQFTGTIKENGEISNLDEIWHFRQFDNRKEWVVGGIQQEVFQP"
     gene            complement(59300..59782)
                     /gene="greA"
                     /locus_tag="U6309_000052"
     CDS             complement(59300..59782)
                     /gene="greA"
                     /locus_tag="U6309_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEN5503524.1"
                     /translation="MSKHPMTVEGAEALKAELHRLKFVDRPRIVEAIATARAHGDLKE
                     NAEYHAAREQQSFNEGRIQELEAKLSHAQIIDISKLPNNGKVIFGSTVTICHVATGSE
                     LTYKIVGEDEADIKLNKISYSSPIARALIGKELDDAVTVETPGGMVEYEIIQVQYIVE
                     "
     gene            complement(59791..62994)
                     /gene="carB"
                     /locus_tag="U6309_000053"
     CDS             complement(59791..62994)
                     /gene="carB"
                     /locus_tag="U6309_000053"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEN5503525.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGTQAVRALKEEGYRV
                     ILVNSNPATIMTDPELADATYIEPVQWKEVARIIEIERPDALLPTMGGQTALNCALDL
                     VREGVLAKYSVEMIGATREAIDRAEDREKFRQLMIKIGLDMPRSAIAHSLEEAIQVQA
                     RLGFPAIIRPSFTMGGSGGGIAYNREEFEEICIRGLELSPTRELLIDESVLGWKEYEM
                     EVVRDKNDNCIIVCTIENFDPMGVHTGDSITVAPAQTLTDKEYQRMRDAAIKVLRAVG
                     VDTGGSNVQFAINPEDGRMLVVEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LKNEITGGKTPASFEPSIDYVVTKIPRFNFDKFPQTPDTLTTQMKSVGEVMAIGSNFQ
                     ESLQKAIRGLEIGRCGLHSLFQKEDMARLRGHLREPTPDRLWYIADAFRQNLSLEEIH
                     LESRVDPWFLAQIQELVLLERSIIGKTIRDLDKTTLHLMKHRGFADAYLAKLLGCTEA
                     EVRARRVELEILPVYKRIDSCAGEFPSDTAYMYSCYEGSCEARPESNKKKIMILGGGP
                     NRIGQGIEFDYCCVHAALSLRSAGYQTIMVNCNPETVSTDFDTSDRLYFEPVTLEDVL
                     SIVAMEQPEGVIVHYGGQTPLKLARDLEANGVKIIGTSSDAIDMAEDRERFQQMVKEL
                     KLHQPDNGTVRSEAEAIELANKIGYPLVVRPSYVLGGRAMEIVYQEEDLRYYLSHAVE
                     VSNDSPVLLDKFLNDAIEVDIDAVCDGKDVLIGAIMEHIEQAGIHSGDSACTLPPFSL
                     GVVVQQDLIEQIRKMALKLGVVGLINAQFAIQADDIYVLEVNPRASRTVPFVSKATGL
                     PLAKIAALCKVGISLKDQGLNQNHHAPSFYSIKLPVFPFIKFSGVDSILGPEMKSTGE
                     VMGIAKRFGQAYAKAQLGAGCHIAKRRRAFVSVRDADKTRVGEIAKRLIELGFEIIAT
                     RGTALALQAAGVDCRRVFKVAEGRPHVLDFIKNNEIDFIVNTTEGKQAIADSFAIRRN
                     ALQHKVSYTTTLSGAEAACLAMKYEDRETVTRLQDLH"
     gene            complement(63123..63395)
                     /locus_tag="U6309_000054"
     CDS             complement(63123..63395)
                     /locus_tag="U6309_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503526.1"
                     /translation="MNEMDILSLFYDEMIARGVTREQVFLSIEEDAAAMLTQKLDKPV
                     SVEEAQKLTDICIANEWLERTTADPYYKYLSLTEAGLQILLSNLYK"
     gene            63508..64689
                     /locus_tag="U6309_000055"
     CDS             63508..64689
                     /locus_tag="U6309_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN5503527.1"
                     /translation="MQEIDVLIIGAGAAGLMCAIEASKRKRKVFVLDHANKAGKKILM
                     SGGGRCNFTNYYIEPNKYFSHNPHFFKSALSRYTQWDFIELVNKHKIAFHEKTLGQLF
                     CDNKSKDIVDMLLKECEQYGATIYLNTVIEQIQKTNDYSFKISTTKGRFHCHSVVIAT
                     GGLSIPTMGASPFAYKIAEQFNIKVWPTRAGLVPFTLDVLEKDRLSVLSGIGVDSLVN
                     NERNQFREHILFTHRGLSGPAILQLSSYWYPGEKICINLLPEHNLLESLKTARAEAPH
                     KQLNSVLSMYLPKRVVEVFIPQKLGEKKLADSSNKDLETISHLLQNWVVKPNGTEGYR
                     TAEVTIGGVDCHAISSKTMEANNVPGLYFIGEALDVTGWLGGYNFQWAWSSGWAAGQV
                     V"
     gene            complement(64730..65482)
                     /locus_tag="U6309_000056"
     CDS             complement(64730..65482)
                     /locus_tag="U6309_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2628 family Dot/Icm T4SS effector"
                     /protein_id="HEN5503528.1"
                     /translation="MAFIRHLLEYLLHPFFLCLLVLGICIFLLYRRLAPTFTKVMLFV
                     VLIVLLAMSTGWLPSYLTYQLESQYPIVKRVNPNVKWVVVLGGGQNIGEGMPANDLLT
                     SASIKRLIEGVRLFRTLPNAKLLLSGGGSAGEQSEALLLTELTSWFAIDSEKVVIETA
                     SINTADQARELAAIVHDQPFYLVTSAIHMPRSMALCQQQGLHPIAAPTDFTFFWSTDT
                     WVKMVIPNSYNLAYTSIALHEILGKIWALGFR"
     gene            complement(65482..66552)
                     /gene="lptG"
                     /locus_tag="U6309_000057"
     CDS             complement(65482..66552)
                     /gene="lptG"
                     /locus_tag="U6309_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEN5503529.1"
                     /translation="MSRKILDRYIAKTVLSAIGLVTLMLVGLQTFILFVNQIDDLGRA
                     DYGIVQASVFVLLQMPYQVYLFFPIASLLGCLVGLGVLANHSELVVMRAAGISIGQIT
                     GAVLKASLIVILLITSLGETIVPVMAHYANEYKVSALTGGQALLTPRGVWVRNGNDFV
                     SIASVTHENTLRYVNQFRFDNQHNLKLARQISEVKYVNNSWIAYDVKQTEFAEDHTKA
                     YHFDTLPWDVPVKPEILKITGTNPDEMTLHELNRYLREQQRNHQNVSNYKFAFFQRIT
                     QPLNTMVMMILAIPFIFGPLRSSTMGSKLLVGAMVGFGFHILNRFFGPVSTVFQWPPE
                     LAAFSPTLIFSLIGIYLMRRVR"
     gene            complement(66549..67628)
                     /gene="lptF"
                     /locus_tag="U6309_000058"
     CDS             complement(66549..67628)
                     /gene="lptF"
                     /locus_tag="U6309_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEN5503530.1"
                     /translation="MIIFRYLTKEVFLTLIALTAILVLIFMSNQFIQYLNRAASGSIP
                     GFVIMKLLMLELPNLMGLLLPLGFYVALLLAYGRLYAESEMTVLRACGYGPKQLLKHS
                     FAMATVVLVVVAIVMIWASPLIAVERTKLIRSTGIKTLVQTIMPGRFHAINSGQQIFY
                     VQSMNRDHSQAEQIFMAKHSVQDNRLLWDVLWADKAFAEVNPQTGEEYIVLQNGKEYQ
                     GVPGHADYQIAEFASYKSKLPNPTVVMNDDARTAKTRDLWPLNNHDLKKAAELQWRIS
                     IPLMVLTLTLVAVPLSRVSPRSGKYSKLLPAIIIYIFYANFMFVSRDAIATGKIPWWI
                     GMWWVHVAVAMLGILFIWRNQVKLA"
     gene            67832..69283
                     /locus_tag="U6309_000059"
     CDS             67832..69283
                     /locus_tag="U6309_000059"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEN5503531.1"
                     /translation="MNYGLTHTPSLTTSECLVLGVFSDLALPDFATAIDNEQQGLIKK
                     LCQRVPEPGNTVWQTDVEGHSLLIIQCGKKEEFSANSLQKRVGEITEALIKQRFSSAT
                     VCLPRLNQESAEWQLEQMIVQIDNLRYQLLDFKTKHAKSHKLESVIFHLPGATEKSLE
                     MAKAIVTGVEFCRDLANMPANICTPTYLGEQAISLSKQFDQISCQVMGPEEIKEMGMG
                     ALLAVAQGSDQPPRLIDIHYHGNKNSAPVILVGKGITFDSGGLSIKPANAMDEMKYDM
                     SGAASVLGVIKACALLKLPINLIGIIASAENLISGSAVKSGDIVTTMSGQTVEIINTD
                     AEGRLVLADALTYAERYNPDFVIDIATLTGAIIVALGNIATGYMTRDEQLAKSIECAA
                     NESQDKVWRMPLDEAYQDALESPLADMINAGFDRSAGSITAACFLSRFTEKYRWAHLD
                     IAGTAWISGKKRNATGRPVPLLIQLLRHVANSR"
     gene            69264..69698
                     /locus_tag="U6309_000060"
     CDS             69264..69698
                     /locus_tag="U6309_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEN5503532.1"
                     /translation="MSPIRVDFYLLASSLPESRWLVACRLIEKAYTKGHTIFVFCDNQ
                     QQAEYLDELLWTFKDDSFIPHNLQGEGPEPPPPVQIGYQKEPRGFTDILLNLSTNIPI
                     FYTRFKRVIELVSNVEHEKEVSRLHYKEYRNKGCALHTHSIE"
     gene            complement(69845..70162)
                     /locus_tag="U6309_000061"
     CDS             complement(69845..70162)
                     /locus_tag="U6309_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HEN5503533.1"
                     /translation="MFVTFSCDAYENITYFENVAKQLLLLMGHGGTIPGAIKSENVLD
                     ALHHLQQGLGKIKSENKPLEDDEDSEPDISLAKRAIPLIHLLQAAEKKGCDVLWSHSK
                     SPG"
     gene            complement(70185..71549)
                     /locus_tag="U6309_000062"
     CDS             complement(70185..71549)
                     /locus_tag="U6309_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEN5503534.1"
                     /translation="MQAEVFYKSCGDRVVPVHLISQSQWDEGIENLTPMERNCFSVRQ
                     FKGKLGDYCFILNADGVIEKAFIGSGTGNQESALANAALLLPPGNYQFQDKLSRESGV
                     VWALAQYRFDEYKKYELQPRVLIVEANDMDYILSLANSLFLVRDLINTPTCDMGPESL
                     AHVVEELAKTHKAYFKQWIGEELIKDNFPAIHAVGRASALAPRLLSLHWGNEKHPRVT
                     LIGKGVCFDSGGLDIKPSSGMRLMKKDMGGAAHVIGLAQWIMVQRLPIRLQVLIPAVE
                     NAIGPNAFRPGDVLTMRNGLTVEIENTDAEGRLILADALVKACEEKPDLIIDFATLTG
                     AARVAVGTEIAAMFSNHDQLAQDIVSASNKVSDPVWRLPLFSPYEELLNSNIADMSNS
                     SSSSYAGAITAGLFLQRFVAKSIPWVHFDIMAWNTVSKPGKPEGGEAMGLRAVAEYLL
                     QTYG"
     gene            complement(71554..73125)
                     /gene="murJ"
                     /locus_tag="U6309_000063"
     CDS             complement(71554..73125)
                     /gene="murJ"
                     /locus_tag="U6309_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEN5503535.1"
                     /translation="MLTTDIMVPKKQSLIRSTSLVSLMTFISRIVGFARDMVLANFFG
                     AQAGMDAFFVAFRIPNFMRRLFAEGAFSQAFVPVLAEYQKTRSAEDVRTFIARISGYL
                     SSILTLVTVVGIVASPVIIFLFAPGFHHDSVRAELATQMLRITFPYLMLISLTAMAGA
                     ILNTYGYFGVPAFTPVLLNISMILAAVYLCPNLPQPVVGLAWGVLIAGIVQLVFQLPF
                     LYQRHLLIRPRVVRDDPGVNKVLKLMIPALFGVSIAQLNLMIDSIFASFLKVGSVSWL
                     YYTDRLTDFPLGVFGVAIATVILPHLSRKHTEQNINQYSRALDWGLRSILLIGIPAGL
                     GLSFFALPLIASCFAYGKFTAYDLLQTQKSLITLAMGVPAFMVVKVLASGFYARQDIS
                     TPVKVGAIAMVVNTLLCFVFVWHLAHAGLTLASALAGYVNCVILLFLLIKRGLFKPSP
                     GWLKYSMQLLIANFAIGVYLYFMSGTATYWLGFSPLMRLSLLLAHVFVTVVIYLLVLG
                     IIGLRPSHFRGHIKD"
     gene            73483..73749
                     /gene="rpsT"
                     /locus_tag="U6309_000064"
     CDS             73483..73749
                     /gene="rpsT"
                     /locus_tag="U6309_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEN5503536.1"
                     /translation="MANIKSAIKRARQNVKLRQHNASARSMYRTYIKNVLKAVESGDQ
                     EAARAAYTKAQPVIDKAANKGLIHKNKAARIKGRLVARLKAMAA"
     gene            complement(73810..75021)
                     /locus_tag="U6309_000065"
     CDS             complement(73810..75021)
                     /locus_tag="U6309_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2637 family Dot/Icm T4SS effector"
                     /protein_id="HEN5503537.1"
                     /translation="MFLTINDLQSQIQLAKKRLYVLAKNWLDTQKEAINSKDKANWLN
                     AFQASTKEAMQEQLKATGFPLIRNAILVNQSEGELPQHSLEQSSSLIHSENFPEQFNS
                     RTVPRVIYVCHSGENNQVIKRLCQLTTLMEQLHTLEELTFEVQTNVHFRGLNLAYRMA
                     RSINKEWRDVEKGRETVEYYAERLQIAEKQYKLASDFCHECLGIYKEQLDARIMYFNE
                     SLNFFVECLAAAFSSVKDDRYWLTKFLLPSLPWHSMLNEVATWLFTTNGFTNPTIDSI
                     EVNRSHLKAITEQFAKLMMAPFCSLEKELQGKIEKVENEQKIAKENVYAMSSELDIVI
                     HMQSQRKLAALLFPAQPLSMLGFFQGVAAAMGRDYFYSTFIKGNQSQEPRITDDEKPL
                     IEASNYSNGLH"
BASE COUNT        23497 a        16470 c        13655 g        21512 t
ORIGIN      
        1 atcctggctc ggcttatgcc ctggcaataa agtctctggt ggaaaacatc ttagcggaaa
       61 aacaaagcca tctaacaata aagccgctgc tactctgcgc atggcagcct ctactttata
      121 ccgcagtcaa acctctctgg gtgcctattt aagaagactt aaatcacgaa taggacctat
      181 gaaggccatt acagcaacag cccataaatt agctattatt atttacaata tgttgcgtca
      241 tggtgtggac tacatagaat ctggtcaagc ttactatgat gaaaaatatc gcgaacgaat
      301 gatcaaaaat ctaaaaagaa aagcaactga gttcgggatg agcctcattg aaaatcagat
      361 aaattttgct ccaaatatca attagatgat ggaagttatt tagaagtcct ggggttatgg
      421 tttactataa tgactgaggt gtttaggttt actggcttgt ctgagtttta tttcgctcca
      481 ataggcccta cttgatttct ggtatttttg gccacgactt tagaactttc tgaagtacca
      541 gcgcatgatt atgttgaaga tcctttgccg cataaaacaa agtgactggt gaagtgcttg
      601 ctaacgctaa aacgtgttct atcaattcgg gtttcttttt taactcctgg gcataccgct
      661 ttgcaaattc atgccaatga tcactgtctt gatgaaacca tttcctcaac gccggacttg
      721 gagctatgtc tttcagccac atatccagag gaaaactctc tttttttatt cctcgaggcc
      781 ataatcggtc tacaagtatc catagccctt ctttatgttc cggtggagag tacactctgg
      841 ataaggtaat ttggggcgtt gtcatactat gtcattcctg gcgttggaaa aatttcagga
      901 tcatgccgat ctctttaatt aacatgagtt atggataaga taagcgttat ctccaatcgt
      961 tcgggctgat gaggcattta tgctgtctcg aagccttgta tggaatttaa tgttgcgtgc
     1021 taaggcttcg agacgatgct tatgcatccc tcagcctgaa cggctcctgg atatcgactg
     1081 cttcttatat aatccttatc cataactcac tttaattaag aatagcatga tggagaaatt
     1141 tttccgctca tccttgcctg ggaaagatta tatccaatca ggtaatttag ggccgcggct
     1201 aatatcaaat ggattaggca aggatgactt atattcttcc attactgaat ccttcgccgc
     1261 ttccgatttt tccggatgtt tcactgattg gttttgatgt tgcaaaactg gtgctttgcc
     1321 gggtagcaaa tttttttcca tcaactgttg cacaaacaaa tcataatatt tagggctggg
     1381 tatctttata gtcaaactat taccctctct ggcgagtgtc attgcttcca gaggaacctg
     1441 tgggtctttt attgaatttt taaaaaggtt aaattcctgt gcaatcgtat catacaactc
     1501 atccagagca ttctgctctt cattgccaag ctcgcgcttg ccttgcaaca ccaataaacc
     1561 aagctccatg ttcagagtca tgttgactaa agcaaggggg tctttaaatt caaaaacaaa
     1621 atcttcatcc tctggcaaat gccataggcc gctatgcaat agtagttgat ctatgtttgt
     1681 gacattggca ggattttttg gtgcgagcaa ttgatgatca ttattatcca ctttgtcatc
     1741 agaagcgcta tccgatccgc cgcctccaga accgccgcca tgaccacgac aaaaaggtaa
     1801 tcctaatgaa cgacagatac tgcagggatt attttcaatt gcacgtgcac gttcatcttt
     1861 ttgcgtttct tctcttttct ttgccacaac cattatttta aaccttggag ttactaacag
     1921 gcagatgata atttaaaatg aatgtttcat ttggcaactt ggtaaatttt gcaaaaaatt
     1981 tacaataaac tacaccttga acctcattac ggagatcaag gcacattttt gggattaggg
     2041 ttcaaggtag gagtatagcg agcttctctt tgctcttcaa tttcatcatc tgttccatct
     2101 tgtcctgttg tttcatatcc aggaacacca tcaccgacta aatccacttc atcaccacga
     2161 gtgaattgat tatgatccat atcattatct gacccttcca acttcatttc ctcagtcaaa
     2221 atatctttag gatccaggac tgtgtctggt tctggctcta actcaatcat attgctgccc
     2281 ttaaatataa tcttctctat atttaagtgt agcagggata gaaagataag aaatttggca
     2341 aggattagtt taacgaatac cttctgcccc ttgctgggaa aagactcaaa aaaccatcgg
     2401 gtgatatgga gttaatgaca ggctaaactg ctgcttcagt tgggcataaa gtctcattgc
     2461 cctataagca aagagacctt ttttcaaaaa atataaccat tattttctat ttctataata
     2521 ggctttttat tctttgtgct tccagtttgg aatgagatat ctgtatatag cgattacaac
     2581 tattcagctc agcatattgc aaagaataaa tggcattatc taattcttgt tttgctgcag
     2641 aatgaacatc ctctacaatc cagtccgccg ctgtatcaat gtgtattgaa gcaataacca
     2701 atttttccgc acaggttttt ttagcctggc tggaaaccaa agaggctact gttgaagcca
     2761 agtcatgaca ctgttgtaaa atggcttctt tattttctga tgaatttgca taactgctta
     2821 aagttagaac ggacaaacag gctatcaaag ttgcttttaa cttcatgtta atcatattaa
     2881 actcctgtaa ttgtttgtat aaaaccgttt tacatcgaat atccataaaa taacccatgc
     2941 caggaccata ccttcctaat tcagcaccat aatctggcgg gcaatcgctc aactctaaaa
     3001 tcaaaaagtt taagcagttc ctctttccat aagaaaaagg aacttgaacc gtaatcatta
     3061 cggattaaga atagttatct tgtggtattc agaactaact cgataaaagc agaaaaatcc
     3121 ttaaccaaaa gcattaattc ccgttatatg tcttcccaag accaaagtat gcacattatc
     3181 ggtaccttca taagtgaaga ccgactccag attcagcata tgccgaatga catgatactc
     3241 aagactaatt ccattcccgc ccattagatt cctacatttt ctcgcaattt tcaatgcttc
     3301 acggcacgca ttacccttag ccagagaaat catagtcggt gtctcacgat gctgatcctt
     3361 taaacgccca atctgcaaat taaggcattg tgctttaata atttctgtat acatatcagc
     3421 caaatctttt tgaatcaact gaaacgaggc caaaggtttg gcaaactgct tacgttccat
     3481 caaataatcc cgagcgatat caaagcaagc catggcagcg cccattgctc cccatgcgat
     3541 accataacgc gcctgactca agcaacttaa ggcagctcca agcccttttt cacttttcgg
     3601 caataaattc tcgtctggaa caaaaacatt atcaaagacc agctccccgg taatggaggc
     3661 tcgtaaagac atctttaact taatttcagg acaactcatg ccttcagact ttgtatcgac
     3721 aataaaacca cgaattcctg cctctgtttt tgcccacact attgcgatgt ctgcgatagg
     3781 ggcgttggta atccacattt tagcgccatt caaaatccag ccaccttcaa tttttttggc
     3841 atgagtacgc atgctggccg ggtcagatcc tgagtctggc tcagttaatc caaagcaacc
     3901 tataatattc cccgcagcca tctctggcag gtagcgtttt ttttgctctt cactgccaaa
     3961 tttaaaaata ggatacatac acaaagaact ttgcacagaa acaaagctgc gcaaaccgct
     4021 atcacctcgc tccaattcct ggcaaactaa accataggca acataagaag cttctgcacc
     4081 cccatattcc gcaggtaaag ttaagcccaa caaacccaga tcagcagatt tttttatcaa
     4141 ctcttttgga aaatgggcat gttcataaga ttcagccatt aaaggtatta cgtcattgct
     4201 aacaaaacga gacacactat ctcgtatcat gcgctcgtca tcatgtaatt gttcatcaag
     4261 aaacaacaag tcgtccactt tccttctccc ttttatctga ctgtaatgcg attaccgcgt
     4321 ctataatact ttcctgactt ggcaataaat actgccaagc agttcctaat ggtatgaagc
     4381 aatctttgcc cgtaatccgc tttattttta accgtggcga agcctcttcc atcaataatg
     4441 tcatcaatcc ttcactaatt gagccgcttt gtctaccctc atcaacaatt aaaatacgtt
     4501 ttgctttggc aatttctttt aaaatggcgt cttttggcaa ggggctgagc cagcgcaaat
     4561 caatgatctt aacagaaata ttatgtgctt cacgcaaaac cttttccgcc tggcgagata
     4621 aataatatcc attggcatat gttaaaataa cagtatcccc ctccccatat acgccaacct
     4681 ctccttgaga aatcatttca tcaggtgacg ggtattcaaa taaccaccca ttatctcccg
     4741 ggctatataa atccttggtc atgtagaggg cgatgggctc taaaaacacg acaacccgtc
     4801 cttcctcata ggcaaggcgt atacatgttc tcagcatttt tgctgcgtcc gggccattag
     4861 aggggcaggc cactataacc cctggcaaat cgcgtaaaac ggctattgaa ttatcgttat
     4921 gaaaatgacc gccaaatccc ttctgataag caagagaggc gattcgaagt accataggat
     4981 tttgatattg gccacttgaa aaaaaagata aagtggatgc ttctccacgt aattgatctt
     5041 cggcattatg cagataagct aagaactgaa tttccggaac aggaataaag ccattatgtg
     5101 ccaaaccaat agcagtacct ataatggtcg tttcatcgag aagggtatca aaaaccctgc
     5161 gttgcccaaa acgagcctgt aaatcagctg taactcgata aactccacct ttctttccta
     5221 cgtcttctcc aaaaatcaac atattaggat actgcatcat cagatcggtc aaagcaaaat
     5281 tgatctgctg gcataaatta cgctttaggg cgagttgatt ataagctccg gcaaatactt
     5341 gcgctcgtct attatcatct ggcaatgagt atttttgctt tttatcaatt cgtggaacaa
     5401 ttgaagccat tatttcttcc gcacaactca ttctgggttc tctgattgcc tccagtgctt
     5461 ttgcttcaat taaagctcgg ttgtcctgat aaagctcaac cattgcctgc aaactcatcc
     5521 aaccctctct gtacaaaatt cctgctgtat gcaataaagg gtcgtttgct tctcgttgct
     5581 caatttcttc ctgagtgttg tattgcgatt caatatcaga accggcatgg cccaacaaac
     5641 ggacgcaacg catgtgcaag aacacgggtt gtttttttac tcttgccaaa tagtccgctt
     5701 cctgcgcttt ggcaaaaaca tcagcaatgt ttaatccatc acactcaatg taatggattc
     5761 ctggacgaga ggagacggat gacttaatcc aactggatgg agtaggcact gagataccga
     5821 ttccattatc ctcacaaata aacacaatcg gtagcggata attttgctgg gttatccaag
     5881 agcaagcatt caatgtggct tggctcgttg catggttggt tgaagcatca ccaaaggaac
     5941 aaagtatcac ggaatcaggc gccagtttac ttggcaatcc taactctctc gcccgggtaa
     6001 ttgataaagc agcacctaaa gcttttggta aatgagatgc gatagtcgac gtttgaggcg
     6061 ggatatttaa agggacgctg ccaaagactt tatgcctgcc ccctgagata ggatcacttg
     6121 ccgcagccac taatgacaac aagatatcct ttactccatc gattccaggt aattgctttg
     6181 ctcgttgcaa gaaaaaggca ccgctgcgat agtgcaaaaa cgccatatca ctcgctctaa
     6241 aaacctgacc caagaccgca ttgccttcat ggccgctact tcctatcgta taaaaagaga
     6301 ggcctttttc ctttaattgt ctcgcaataa tatcaagaag ccgagattta atttgtgagt
     6361 caaaaaattc tatagcagtt ttcttgtcca actctgctgt atcggggctc atgctgcttc
     6421 ttaattgtgg gaaatcccct tgcaacactc ttttcagaaa ttgttcatcc actacacttg
     6481 ctctatctaa catcaccaca ccttgtttaa attgcgcaat gaccagatga ataaggctat
     6541 ttcaattaca ttttgtgtaa tgattactct tatatccatg cctggtatcc tttcccaacg
     6601 aactataaag aaattcataa ttctgccgat aattaagacg atgagtatat acttattaga
     6661 caacttacga aacagtccag cgggggacga cgataagaaa aagcagtcta actgatgata
     6721 aggatggtgt atgtcggtcg agatgagcaa aacctccagt gtcagattcc ctcctggaca
     6781 agaaatcaaa ttagcaaaac cggcgacaga actttatgta ggacaaattc tgaaaacggt
     6841 tgttgtcaca acactgtcta atgatcaggt tcttatcaat attaatggac aaaatctcaa
     6901 cgcaaaggca tctcatcatt ttacaccggg agaattattt gaagtaaaag tcatagcgaa
     6961 ccaccctgaa acaatccttg aagtgcaaca aaaaacaccg ctttcatctc tattacaaaa
     7021 tgcgttactt cagtatttac caaaacaagc accagctacc aatcttatac agacactgac
     7081 tcaattgatg gcagaaaata atttatcggc tcccatcaat caggttatga aaaccatttt
     7141 aggcaatatc cctgttttga ctcaattacc taatcaactt atgcaagcga ttagtcaaag
     7201 tggtgtgttc ttagaatcca aattgttaaa aggaaagata ccccgagaaa tacaatcgga
     7261 ttttaaaggg ttgtgtttta aattattaca cagtttaggc acagacagta aactcccaat
     7321 caatctgccg aacgaattgg aatcaggttt tcttcagcaa gcgccccttc ctttaccagg
     7381 agctatccct caaccattga ctaaagacac gatattgaat cttattggac tatcaacaga
     7441 ggcggtacac catattttac gtgagcaagt aacacaggca ttagcgcgaa tcacgacaaa
     7501 tcaaattaat tatttatcgc aggagaatca agaagcctat ctaatcatgc ttgattttcc
     7561 tttaaaaacc cccgatggca ttgatgtaat ccctattatg atcaaacaac acaaggcaga
     7621 acctatgcaa ctgtctaaat ggtcaatatc gttcgctttg aatttatccc atttaggaag
     7681 catacaagct acagtttcga tttacgctaa aaacatcgat atcaaggtta atactcagca
     7741 atcgtcaaca ttgcaacttc tcaatgatta ccataaggat ttggaagaaa cgctctatga
     7801 atcgggtctt cacttaagag aatggaagct gcaagttggg ttggaagaaa atcatatcga
     7861 tgccacaaac cttcgtttat tggatataaa aatatgaaaa gaaagaaacc tgtcgctgtt
     7921 gcattacgct atgatggcaa ttctcctccc aaagtaaccg ctaaaggaga aggtttattt
     7981 gctcagcaaa ttatcaatac agccaaaaaa catggcattc ctctcgaaca aaatcccgaa
     8041 ttaacagcgc tcttatctca agttcgcctc aatgaagaaa tacccaaagc gctttatatt
     8101 gccgtagccc aaattttggc attcctttat tacatcaatg gaaaacactc caaagaataa
     8161 atccatttca aacgaatttg aacttcttca gatgaacaaa cacgcaaagg catccataaa
     8221 acattggcaa tatctgaaca aataatctac aatttggtta agttatgacg agaggtttcg
     8281 ctatgaaatg gataatgctg atcgttgtta ttataacaac tgcaggttgc tgttgtacta
     8341 cagcagaagt agttgaatac caacaagtga cagttgccgc tccagtggtt gagcccgtca
     8401 ttttagatgt cgatgatccg gagcctttgg atgtgactac caccactatt gagttttact
     8461 aggaaattat caggcttcac tactttctga gaatagtact aaattctatt tttcagtaca
     8521 gtctttaagt gaagccctac gcaatcactc aatgcaagct aaatttgtat caaaaaaact
     8581 tattaatctt acgttaatgt ttgccagcta taataaaata aatgttatga tatggaataa
     8641 accatgccac gaatcactga aaatatagaa acctatgtaa tgcatttatt aagtgatctg
     8701 gaacccgttc cttctcaaaa tcagcatctt gcctacggtt atcccggcga aagacaggta
     8761 ttcaaagacc cgatgctaga cgggaaacaa gtcgtggtgg taaacagtca atatgataaa
     8821 catggcaagc ctgttacagg gcaacctgat gtgatccaag aggcgaataa ttatatagac
     8881 aatcttgtag ccgctgccaa atctctgata gacaaagata agaaaggtga gaaggatttg
     8941 agaaagaatg ccattgatga aatggcaaaa actttcaaaa agtccatttc agaaaccatt
     9001 ccttctgaca aagcaaaagc ggatttgttt tcaagtaaaa aaagttctgc caataaagac
     9061 tttctggaac agttggccaa agtcgaaggc agtttacagc aattcacaca ggctgtcgca
     9121 aaagcctcag gtcataaatt aaaagcactg gataaagaag ggcataatat tcgcagccat
     9181 aatcgcgata ccttgataca tcgctttaaa gcgcccaact ctaaagaagg cgaagaacaa
     9241 tttattatgt acattccgtg tggaaggtac accaaacgtc aacaaagact aatgggcaag
     9301 gatgaatctg aagtcaaccg agatcacgac actacaagtc ataaacgctc tgacctggtt
     9361 ctgggcaaca gcagtatggc gcgcatgatt gccggaacaa ggcattctga tggcaccgtg
     9421 actattcacc atgacagttt ttctggtcct ggggcacgca tgccctattc tgattttaag
     9481 ggtgctgatg actataaaaa gctggctatc aaagcggtca ccttaatcaa ccaggaagaa
     9541 atcatccaaa ccctggcaca gcgtcaaatt gaccgaatga ccaatgaaga cctttggaat
     9601 aaaattcctg aagagtaccg ccctgatgag cttcctcctg atgcggagaa agcacgagcg
     9661 caattaataa agctgtatgt agaacataat ccattatctg tcacggaatg ttatacacaa
     9721 gttgtaacag ccgggcaaag ggtagcggcc gaaaatcaaa aagaacaatt tgaatacgta
     9781 cggcaaatga tggatgcttt tgatggctcc aaagcaaaaa tcaccattca aaccggctcc
     9841 aatacagaag tggaaacaga ggttggttat caggcgcgta tgagttcctg gggagtgaat
     9901 tggtttagac aggttggagc gctcaatccg ctctctgaca ataatgtcac aaaaaatcaa
     9961 aatgcgcgtt ttgtaaatca aatgactggt gacgtcatca gcaatttaga caaagtggta
    10021 caaaacctgg gtgattatga caaagcaggc gcattacaca cgttattaaa aggcccagac
    10081 gtcagcgacc ttaatcaaca aatcaccggg aaagaaaatg ccttaaaaga ggtgaaagga
    10141 gcctatcgag aagcattatt cagctatttc gaagaatatc agaaaggcga agggaaatgg
    10201 gatcaagcca agctggagca attgaaaaat caggttgatg gctatgaaaa aagcattaaa
    10261 aaacaagaat cagcaattta tgaactgcat aatcaaatcg atgccttaag aaaggcttat
    10321 tacacagaac acaaagggca aatcaataag gctttacaag aacttaaaga acaaataagc
    10381 cctgtaatcc agaataaaga aacagatcct gaaaccaaat ccaaactgca gcacttttac
    10441 aattcatgcg cttacctcat gcaagctcaa gagctctatt atgagaacac ctggcatcat
    10501 ggcaaaaata atttcaagct gcaaacctta atggcttctc tctcctgcga gctggattac
    10561 gccaatacga aaggatgtaa gagtaataat gatcgtggcc aaagattagc gcaaaaaatt
    10621 gttggcaatg ctttatggac agcaatgagc gaggacgggt tatacactgg tgaatttctg
    10681 gatcatcgaa ggacacatgg taaagacgtc agtaaagtag aacaacttga tcgggaactt
    10741 actaccatac aggcccttca tcatactgcc aatacgggtg tttccggcgg aaagtttgaa
    10801 attcaggata aagccaattt cgctgataat ggccttttcg gcaaggtcgc taattttgcc
    10861 aaagtaaaag aaatggggcc tgaaaacgct tttaccaaaa atatgggtac aaaaattaaa
    10921 gcgggcatcg ggcttggatt gctagcggct ggtgggttag tcgcccttac tttactattc
    10981 cctccggcag gcttggctgt ggccgcattg ggaattggtg tggcagccgg tgctgccatt
    11041 gttgctggcg ttacttttgc ggccacttat ctttttggta ccgctattga tgccattaat
    11101 aacaagatag aagaaggaaa gattaaatcc attgttcgcg agggtatgaa agcggcatca
    11161 gaaaattcac cacaacacaa aaccgaacaa aaggatgatt taacggtgaa tgccaaactt
    11221 catcaacaat taggtgtgac gcgagagtct gtcttggaaa gacaatccac tccaaaacaa
    11281 gagtttgaaa aacccaaaga tatgacctcg aaagttgact taacaccagc gatggaggat
    11341 gataagcgaa gcgaaagcac ccccacgcca gtaaacttct aagggttatg agtttaacaa
    11401 tctaacaatg aaaaaaatca gcgctcttat ttttctttat ctcataagca cggccagttt
    11461 ttctcttccc aaaggctttg tttatttaca cgatgtagcg cctgatatca ttgaagacat
    11521 cagatatgcc acccacaata atttcatagg aaatcctatc ccaggttaca aacgcggagt
    11581 atgtatagtc accagacaaa ccgcgcagca attaaaaaaa gcggaagaat acatcaaatc
    11641 caaaggatat acgcttaaag tatatgattg ctacaggcca caaagggctg tggattattt
    11701 ttataagtgg agccgcaatg ccaaagataa tcgcatgaaa cctgaatttt atccccgtga
    11761 aatgaaacaa gacttatttg accgtggtta tattgctcta acctctggcc acacgcgagg
    11821 cagtaccctt gacttaactc tggtgaaact aacggcctct caagaaaaaa aacctaatca
    11881 tcctttaaca cgttgctacg ataaaaagcc agactatctt gatgacgatt cgatcgatac
    11941 aggaactcgc tttgattgtc tggatgtgtc agccaacatt gattattcca ggctgagtaa
    12001 aaaccagaaa gccaatcgca aattattaca aaaactcatg agccgctttg gttttagacc
    12061 ttattactat gaatggtggc atttcaccct gaaaaacgaa ccttatccag acacctattt
    12121 caattttcca gtcgaataaa tagttaacat cagaattaac tctgacccgc ttattcatgt
    12181 cctggatgaa acagatcaat ccaacccgga agacaattaa aacccaatat gggtttattt
    12241 tttctttagt gggcatatta aatatatatt gaatattatt tgcgcatcag ttattcttgc
    12301 cagccatttc aagtccatat cactaacgga gaatgataga tgcgtgtttc aaaattactt
    12361 tttttaactt tggcattgag cgggaattta tttgctcaag atatcaaagt agtaggaacg
    12421 ttaaatcaga cattaaaagc cccgcaaaat aaaacactcc atacaaaacc cgccatacaa
    12481 caaatcaaat tgcttaaaat gcagttgtca aattctgcta tcaaagcact tgcgagaaaa
    12541 acagacatta cactcaaaaa accgaatagc ataaccacat caacagctct cccatccaaa
    12601 gtagaattag gcatgaataa cgtacctgtt ctgaaccaag gaagctttgg aacttgcgtc
    12661 acttttgcaa cgactgcagc cgtagatgct gctttaaaca aaggtgacta catcagccaa
    12721 ttgtgccaat tacaactagg actgtatctg gagaaaaacg gtcattcacc aagtggttgg
    12781 gatggctcat tagggcgcat agtcttaagt caaatggaaa attttgggat agtcagcaaa
    12841 gaaaaacaaa aaaacctggg atgtggtgga ctaactgaat acccagacaa cgagcaacct
    12901 acccctgatc atgccatgtc gcttgagcaa ttccatgaaa tgagcgagga tccagagact
    12961 aatcctgtta tttacacccc catcctggat gtttatcagg ctgtattaga cagaacagac
    13021 accaacaaaa cgattaatga tataaaaacc gctttgaatc aaaaagatcg ggttactttt
    13081 gcagtattat tgctggattt tgatttgggt gttatgggtg ccgtaggaac gaataaaaca
    13141 caattcgata cctgggtact aacccctgaa attgctcgtg atatttatct tcgcccattt
    13201 tttggcggcc atgaaatgat tatcactgga tatgatgaca atgccatcgc tgttgatgac
    13261 aatggccggg agcacaaagg attatttact ttacgtaact catggggtga gcaatttgga
    13321 gacaagggta atttttacat gtcctatgat tacttcaagg ttttaactat tgaggcacaa
    13381 cgtatacgtg ctcttgctga taatgaatca gaagaagagt caatgacggc ctgaacgact
    13441 caccatcata aaatcaatat aattggttaa gctaaactcc cctcatcctg gttcattttg
    13501 caagtggatt aggtgagggt attcctaaat caaacttgat tcagctattt tgcgtcctcc
    13561 gcaataaaaa tttcaccaga agagcagccc aaaacatacc atttaaaaat cattttgtgt
    13621 ataatgtgat cacgttattt ttcttgcaaa tatcatggcc caaaaaccaa ctaaaatcac
    13681 acaccttgta gtattgggag atagtttatc cgatagagga acgcttaata aacgtgagct
    13741 gctgggtttt atccccatga gttatttaag tggattaaga agcaaatctc caaaaggtcg
    13801 attcactaat ggttttcttt ggggggatta tgtcggtgcg accactgcag agcaatttga
    13861 gatagagcat atccgaagaa aattaagatt gcataataat gcaagagaca atgcggatat
    13921 aggcgatgaa ttactcatca atgataaggc gcgaagagaa aatgaaaact cttttagctt
    13981 aaatgaagac aatcatattc tgtttaaagg ggaaagattt gcccgcttct attgcgaagg
    14041 tggattaact tctcatgact actcttgtag ctttaccttt aacctggttc tgttctttac
    14101 aagattgatt ctcgctactt tgggaggaaa acggactcaa ttattggctg atgataaaaa
    14161 atacaatatt tccaatcttg aaaaatctga aacattagtt gtggagtggt caggcgccaa
    14221 cgacttgatc accgccaatg acaagccttc tcgtgcggct gcagagaaag ccgttaacga
    14281 ccgaattcaa aatattgaag cgcttattca taatgggtac agaaattttg ttttattcaa
    14341 tttgcccaat ctctcattga ccccaagatt tcaaagaaaa aatgctgaag aacagcgtaa
    14401 tgcatctgat tgtactgaat actttaatca gcaactgcag acaaaatctc ttgaattaat
    14461 caaaaaatac aaagaattgg gaattcctgt caatctctcc gtctttgatg ttaatgagca
    14521 gtttaaggaa gtttacagta atcccgaaca atatggcttt gatagagaca agttaaaatc
    14581 cccttacgtt gaatcggatc tttttaaaga aaatcagaaa aaccctgttg atcaaaaaga
    14641 acatatttca ccagccaaag gatatatgtt ctgggatgat gtgcacccga ctgcaacaat
    14701 gcatgactgg ttagctgagc gtttcaaaga gaaatactac gataaagttt ttcgatttga
    14761 accaccgctt caggcaaagc ataggcacaa agaaactgct gatgctctgg caaaaacaac
    14821 ggactctatg ataaaaaccg ataagccgat aaagaagctt ccggaagata ttgtattgat
    14881 gttgaatgct atccattcaa gagcacaatt gatgtgtcaa tccactgatg ctcaacgacg
    14941 tgaaaaaggt gaattattaa agcattttat ttttgaaata aaatgccaat atggaaacct
    15001 tgaaaacatc tatgggttaa tctctgcatt taccttaaac cctgatcatt caaaaataat
    15061 aaaaacccac cagaatccaa tgattgattt ctttagaaat aaggtaacga caagaagcga
    15121 agatgatatt gctgccttgc aaaaagcggt cgcaacgtat ttacaaccta aagaaagcaa
    15181 tgaacatctc gttaaactta cctgataaac gaaatcttaa cttaattgat ctggtaagtt
    15241 ttaatgcatc cctgataggg aacacgcgta cttttcaccc cataaaatag tcctgcttcc
    15301 agattattgc atacttgctc tggacttaag gtgtccaggc actggggtat cgttttattt
    15361 tgacaatttg gaaaaatggc gtcaggaatg ttcatggctt gataatgctc attgccccac
    15421 tcatacaaat cggacgtaaa ataagtagta aaatttctga ccctcatttg cttgtctaaa
    15481 tcaaaaattt tcatcccggg attgttgtga tgcgccctgc taattccggg agtagaaaac
    15541 gcataaatag ttgagccatc ggataatttg attttcctga attcctccat atgggagtgt
    15601 gagaacagta tggtgatttg atcataggat tgatgatatt tgtctaataa ggtcaaaaat
    15661 ctatccagat aaatgtcatg ccaaaaccgg ttgccgttat aggctgtacc aggaggaaca
    15721 tgcatcgcca acaacaattg ctttgcacga tgatttttta attgttgttc aagccaaaat
    15781 aattgtacaa aggcatccct ttgttgatta gggtactttg gtaaaaaaac aggggtcttg
    15841 gtccattgaa ccgaattaag tgctattaaa ataatctctt tattattggg gataacataa
    15901 ctggagtaat agccatcctt gcgcatatga gtatcatcaa taattaaccc atcacaatga
    15961 acgcaagcac catcccaatc cagagccaaa ttgagtggcg acttattgtc tgaagaaaat
    16021 ggctggtaat tgccaagcaa ggaatcatta tttcctgtaa tataaaacat gggcttttga
    16081 cccggattcg cttcatgcaa accatgaaat acaacttgtt cgtattcttc ttttttagat
    16141 gtcgaaaata acgagtgagt aggcagatca cccaaatgta aaataaaatc tacattttga
    16201 gacaattcct caaatcgttt gaacgtgaca tccaaaaact cctttccagt atcctgccca
    16261 tcctttgcta tattttctgc accataatga atatcagaga ttgttagaaa ctttggtgca
    16321 gaaaaggcta aagaaacaaa acatgttaac aaaagaatta cccgaatact aaaattcaat
    16381 tactactcca aaccaattta taccgaaatt attcagttct caattatagc tcttttatac
    16441 aggacttacg ataaacctca atctctttgt ttaaaaatgt ttttaattcg gccatttagt
    16501 ttatctaaac tcccgcatta aaaacatttt tgccttgacc ttggagtttt cgtaagtact
    16561 gtcacaatca ccttgaatac tgcaaagccg gaatgcagaa aaaacagtct atttaacatg
    16621 agttataccg ataatgatta cacgagaaca agttgatacc cagggggccg tccatgctga
    16681 ggaaatgcat aagcatcgtc ttgaagccct ggcacgaaat atgaaatccc atacaaggct
    16741 tcgagacggc ataaatgcct cctcagccca aacggttcga gataacaact gatcttatcc
    16801 ataacttcac gttatttaaa tcatgttttg ggcagaacag cacattcaaa acccaatccc
    16861 tgatgatgcc tcagctcaaa tcaattcaaa atcagttgat caggaccgca gacatgtttg
    16921 cagcgcgaca ataaatggat aaggattata tgagaaccag tcgatatcca ggggccgttc
    16981 aggctgagga gatgcataag catcatctcg aagccttggc acaaaatatt aaatctcata
    17041 caaggcttcg aaacgacata aatgcctcct gagcccaaac ggttcgagat aacgactgat
    17101 cttatccata acttatggaa cgattgtact agccccgcat tgcattttag taatacgagg
    17161 ctggtctttt ttttaattat caaactcggg ttataccgct tcccgaatta cccaggttct
    17221 ctctgcctta ttttcagata aaggtgcttg cacccaaaga agcaaaggtt ttcccgatgg
    17281 tgtaacaggc atcactttag aagacaaggc tacggcgaat gaatatttct tgccaattga
    17341 ttgtaaagct ttccaaacgc tattcgcatt agactgattg tctgtcacga ttaattcatt
    17401 gccccggtaa accacattca catcctgtcc tctcaagacc tgcctcactg ccgtttccaa
    17461 acgcctccat ttggcttgat gaactctctg taactcaatt tgtgtaggat taaattggaa
    17521 cttaatgcga ctatggggag ataatacccg tgccagtcga ctgcttgttg ggctttgctg
    17581 caagaaataa gtgcataatg cgtctaataa tggacgtcct ggcccagctg gttttccggg
    17641 taatctattg atatacatag caaaagccaa agtatggcca ttagcagtat acaagtaacc
    17701 ggataaactg ttcatacccg tcatcgtccc ggttttggcc cgcacaaatc cttgttgatt
    17761 tggggtttta aaccgttttt gcaaagtacc atcccgacca gagatgggta aagcagcaat
    17821 gtattcataa gataatggga aacgttgata caagaatttt aaaagcgcca tggtttgcgc
    17881 tggagttacc aaattataac gtgataatcc agaaccatct gtaaaattcg agtcttttaa
    17941 atcgattcct gtttgttgct gcaaaaaatt tttaattact ggctgtgctt gcttccaatc
    18001 tacaggactg cccttaattt tggcagcagc atgcaagtac aaactatcag catataaatt
    18061 atcagaaggt tttaaagtat ctgccatcag ttgggaaatt ggctttgaat agcgagtggc
    18121 aattaataaa gtaccagcag gcgctcttcc aagcaaaacc tgtccattta atttaatatt
    18181 agacttggca agctcactca caatcatccc ctgggcataa acaaaaggat tctttatagc
    18241 gatgcgttgc tgaacagccc attgccctaa tccaacacag cctcttaccg ttaaattgtt
    18301 ctcaggatcc aaataaaacc ctacaccaca tcctttctca ctcgccttcg tggtcgcttg
    18361 attattcagg ttaatagtac cgccgccatc atctacttca acaatagcag gagcgcctgc
    18421 tttagccccg gggttgaccg tgattgttaa tcggttggaa tcaaccatca aaggtgctat
    18481 tggcgcacca taactataag ataaatcaga agttagccag cctgggggat aggcgggaat
    18541 ggacattagg ctactgtcaa tgattacatt accttgaatg gttgtgatat tccaatcctt
    18601 gagtgaagaa agcaacgttt ttaaatcttc tctgctaaac gaaggatcgc cgctgagatg
    18661 taaatacaag tttccgtgca aaacaccttg ttgtaattga ttggcgctcg tactgagttg
    18721 attcttgaat tggtagtctg gacccaaagc catcagagcc gctgcctcgg aaaaaagctt
    18781 catgttgctg gcaggaatat acaaacgatt ggcattgcgt ttatatagcg tctcgcccga
    18841 agttagatcg gtcactacta tccccaggtt cacatgaggg tttagccgat taattaattt
    18901 atccacctcg aactgcatac tagccgcatg agatgacagt gaccatactg cccacatgaa
    18961 tgccccagtc aacgtccttt tcatttaaat attttcctta acattgaatt tcacaatata
    19021 aatcagtttg aattgtttga tacatagtga caatttctga gttagttcca ataagctaat
    19081 catatctcaa gcccaatcgc tccaaaagcc ctgcaagggt atcattatca aaaaatttta
    19141 cttttaacca acctccgtct tcgccatcgt ttacaatttg tacaggagcc cccacttgtt
    19201 cagccaggat agtctgtaac cgctcaatat ctctgtcttt tctgggattt ttgggactgt
    19261 tcgtatcttt atttttataa gatttaattt cttgctctaa gtgtctgaca gaccattcct
    19321 ctccaataac ctggtgagca aacatttctt gctgttcagg atttaagcca accaatacac
    19381 gagcatggcc aaaggataat actttgtctc gaaccaactg tttaacctta tctgtcaagg
    19441 taaggagacg aagaatattt gcgacatggc tacgagattt acctaccaac gctgcgatct
    19501 cgtcctgatg ataatggaat tcttcaatta aacgcttata accagtggct tcctcgatta
    19561 aattcaaatc ctgacgttga atattttcga ttaaagtgag agcacatgcc tgttgatcac
    19621 tataatctcc aatcaaacaa gggacttcat ttaatcccgc caatttggcc gcacgccacc
    19681 ttctttcccc cgcaataatt tcatatcgct cttttgctat gggtcttacc accaagggtt
    19741 caattaaacc ctgggaagaa attgactgag ccaattcctg taactccgtt acattaaaat
    19801 cttgcctggg ttgatactga cctgcctgca ggcttgtaac aggtaaataa gtaaatcggg
    19861 tttgcatagg aaaaaatcaa gaaacttaag gatatgaaat tgtttcacaa tagtgcctga
    19921 aatggaatta tatccggttt atctgacctc aaagaaaaac cctgaaacga gaaaaaactt
    19981 attctaaaca gtgggtctgt ttattatttt ttcctcattt tccttgttac tttcagattg
    20041 aactccaaaa aatcgaagag tggactgata aggtatttct ttagatttgg agtttaactc
    20101 ttcagcgaag tcgggatatt tttctaataa tttcttgcca agcccaataa tatacgaatc
    20161 cttggagctt tgaatgaggc gcatataatg tgcagcggta atcaagtcat ttgcagctcc
    20221 ggatttcaat aatatctcac aagcagataa catagcgttc gtgcaacctt gggcagctga
    20281 ttgcaaaatc atctgcttat gctgtgatga tgaaggagaa taaacttgtg ccagacgaaa
    20341 taaggccaaa gaattcgttt tagctaataa ctgcatttca ggcagacgct tcaccacggc
    20401 ttgctttaat cccggattat cgggttctcg ttgtaattgt ttaaccaaag cagcaaattc
    20461 gctacttgga tctaattgcg cggccatacc tcaacctttg attatcatct tcattaaaga
    20521 cagaataaat gctcaaaatt aagacaaaat taagagatgg cacttttaca ttttttttac
    20581 acataactgt ttttttaaca gtattttaag agcataaatg ttaaaaaaac tcccatcttg
    20641 atcaaaacaa ggaatgaaaa ttaagataat ttctcatacc tgtcccaacc caaatgaaat
    20701 agcatgtgtg ctaaaatggc tgaggtcagt ccatattgcc aaaataaaaa accaaagagt
    20761 atggactgtg aaagactcaa taatacaaaa gagtaaagaa atcgtctgga tggggtgcaa
    20821 cctgctgcaa tataagctgg aagttggcct acagcaaaaa taaatccact caaaaatatt
    20881 gaaataatta taatttccga attaatttga cttgtaaaca atagagcaaa aaaacacact
    20941 acattcatta atccccatct ggcaattacc tcttctacga cccctccata aaggacacaa
    21001 ccatcaagtc ctattttgga ccgtaattcc gacataatct tgaaactttt ttcatcaagt
    21061 atggaaccaa acaacccata atacagtata caaaaaataa ccaaccctaa aagggcatat
    21121 aaaaaagtag gtaataaaat cacttgaaag gaatccaggc tggctttgcc ttgcaaaaga
    21181 ccttccagga gaggatcgcc tagcccggta cgtgaagaca ggatggagcc agcaaaactc
    21241 atgacaaatg tcataaataa ggtttgcagc acaacaaaac gactaaatct tttttttaat
    21301 ctctcggtat tatcggctag cagaaaatat accaggcgat gcatcacgat taaaacacca
    21361 ggaatggata aacaaaacaa gacaataatt acaggccagt caattatcat ttttcatcct
    21421 ttcgcaatac actgtaaaaa aagccatcca tgccgtgctc cccaggcaat atctgctgac
    21481 catgcccagt ccaccttccc caggaccatt tgccatcact cacttgacaa tccggatgct
    21541 ctgcaacaaa tcgggagatt tgcaactcat tttcatcact cataatagag catgtggcat
    21601 aaaccattaa accacctggt gcaagcagtg gccataatga attcaacatg ctggattgta
    21661 cctgagaaat tgctgaaatc tcttcatccg ttcgtaataa tttgatatca gagtggcgtc
    21721 taatcactcc tgtagcggaa cagggcgcat ccagtaaaat acgatcaaat ggatgaccat
    21781 cccaccagct tctggattcc aatacatcac cttgcactac cgttgcttgc aaatccaatc
    21841 gatttaagtt atctctgacc cgctgtaagc gctgtgggtc taaatccaat gcaacacagg
    21901 aagccaaacc agactctttc tccaaaatat gacatgtttt tccgccagga gcgcaacaag
    21961 catccaatac tcgctgcccg ggttttaaag acaataaata cgctgctaat tgaggtgcag
    22021 catcctgaac agacacagaa cctttagcaa aaccaggaag cgtcatcaca tcacaaggga
    22081 ttgccaaagt aattccatca ggtgcgacag ggtgtttaaa agcctctatc ccggattttt
    22141 ttagtaaatc aagatactca tcaactgagt ttttcaacac attgactctc aaaatcatgg
    22201 gtgggtgtct atcattcgcc tcagcgatga attgccaatc gtttggccag tctttttgca
    22261 ggcgttgaag tagccaattc ggctgtccat agataaaaac cggttctgct gccagctttt
    22321 ccagaatatg gtcttgtcga cggcaaaaat tccttagcac ggcattcacc agtccttttg
    22381 cccaactttt tttaacctgc cccagtaaag acactgtctc cttcaccacg gcataagccg
    22441 gcttattcat atactgcaat tgataaatac ctatcaacaa gactatccat acctcattat
    22501 ctttaggtcg cttattgatt aaagtatcag ctatggattg taaacgaaaa taatgacgac
    22561 aaaatccata gcaaatttct tttgtcatgg gagaaatctc ttgcggagac atcaaatagg
    22621 acaggttttt tctgctttct atcaaagccg tcaaaatttt taaggcgttg aatctgtcat
    22681 ttactttcat tgcaacatta aaccagtatg caattgtgtt ttacccgaat ttaaccaatc
    22741 cctaactgaa atgacctttg ctccgggaaa ttgaatcttt tccactaaca atgcattatc
    22801 attggtagcg accaacattc catttttatc gatatttaat accatgcccg gcatctcggt
    22861 actcatgata tcagttgctt ttgcctggtg aattctgagc attaattccc cagccagcgt
    22921 ataggcaaca ggccatggat taaatgcgcg aatttttcta tctatttcca ctgcagactg
    22981 atgccagtta attcgtgcct cttctttatt aattttacca gcataagtcg ctaactcatt
    23041 gttttgtaat tcaaattgtg ctgaattgga tgctaaagcc tccaaaacat ccaataatgg
    23101 ttttactgac atttttgcta atttatcatg caaactacca gcggtatccg aggaagtcac
    23161 aggacaggtg gccttgcata acataggacc tgtatccaat ccaacatcca tttgcataat
    23221 ggtcactccg gattcagcat caccatgtaa aatggcatgt tgtattggag atgcgccacg
    23281 ccagcgcggt aacagagaag catgtacatt aatacaaccc aaacgaggaa tttccaacac
    23341 tgcttttggt aatattaatc cataggctat cacgaccatt acatcgggct ttaaggcaga
    23401 aagttcatcg atagctcctt gatttttaaa attaagaggt tgataaactg gtatctggtg
    23461 atttattgcc cattctttta caggagactc ctgtaattta cggcctcggc cagccggcct
    23521 gtcgggctga gtataaacag ccttgagatg atgcctcgat tgaattaaag catccagaca
    23581 gggtaagcca aattctggag ttccagcaaa aacgacagtt aaaccattca tggcttacga
    23641 gcttgtaaac gcttgaactt atctaatttc cggcgagcca tcattctctt caatggagac
    23701 aacatatcga caaacaattt gccattcata tggtcaattt catgttgtaa acactcggca
    23761 agcagccctt cacccgtaat ttcaaacggt ttgccgaacc tgtccagtgc cttcacggtc
    23821 actttttcag ctctgacaac ggtatcataa gcccctggga cagacaaaca gccttcttca
    23881 aattcttttt caccatgaga ggaaactatt tcaggattaa caataacgat ctgctccttt
    23941 ttatctccca ctatatcaat aacagataaa cgcaagctga ctcctatttg tggtgcggca
    24001 agcccaactc cccgagcatc atacattgta tcaaacatat cgttgattaa tgtctgtaaa
    24061 ctctcatcaa aagtctcgac cggcttagct atttttctta agcgctcatc aggtaaataa
    24121 agaatcttgc gaattgccat tgtttatcta gttctccaca cgttaatcgt gatattatcc
    24181 ccgatataca gtaatgctgc aagatgctaa ggctcaacta aagggattga tacatgagat
    24241 attgtctcct cattttctgt ttcatcatat catccattac tcatgcctta agcctaagac
    24301 cggattcccc ctctcgttat gttgtgcaac ctggagacac cttatggagt atctccagcc
    24361 gttttttgaa taacccctgg gaatggaaag cgttatggcg cgcaaacccc cagattcaaa
    24421 atcccgacag gttatatcct ggtgccgttc tcgctttaga gtattatcaa aacattcctt
    24481 atctcagggt tctgtctaac ggaacaatca aactttcccc caacataaga gtgacacccc
    24541 aggaagacgc cgttccacca attccattag gcgatatcaa accttttttg gatgaatcat
    24601 taatcctgga tgttaatgtc ctaaaccgag caccgtatgt ggtagcactc atgagtgagc
    24661 gaatgcttgg aggccaggga gatgaaattt atgtcagagg cttgcatccc tccaaagaaa
    24721 tgcctcaagg aggcactata ggatactcaa tcttcagggg agggagaaat tattttgatc
    24781 caataaccca tgaattgtta ggctataaag cagtcttggt cggttatgga gaattggttg
    24841 ctggcggcga gcccgctacg gttttattga ccagtattaa tcagggcatc aaaatcaatg
    24901 ataaggtatt gattaataac caccctgaat ttgaattgta tttcgaaccc gaaactcctg
    24961 ccaggcaagt cagaggatac attattgaaa tgcctgacaa tatgcctgtt ggaaatacac
    25021 aagaggctgt tggaggagtc atcatcatta atcttggaga aacctccggg ataaaaccgg
    25081 gagatgtttt ggctatctat ggcaaggaac gtattgtcaa tgatcctcaa aatcatctca
    25141 gacctattac cttaccgcaa gaacgtcttg gcgaagctat ggtgtttcgt gtgtttacaa
    25201 aagcaagtta tgccctgata gtaagatcca cgcgtgctat tcatttatta gatacagtaa
    25261 cgaacccatg aataatctgc aacccctcct cgccttaaac aggatgaaaa gaattggccc
    25321 ccgaaccgta ttaaaactgc aaaaaagatg gcctgattta aaccttatgt tccaactgtc
    25381 cagtgttgat ctggaagaag caggcttgcc atcctggttg gcacaaacca taaaaaattt
    25441 tgatccggac tttataaaaa ctgatttgaa ctggctaagt tcatcagaaa atcatcatat
    25501 tttaacttgg gactccccct attatcccgc attgctcaaa gaaatagccg atcctccctt
    25561 cattttatac gctaaaggtg agctgtccgc cttgacacaa cccagcctgg ctattgttgg
    25621 aagtcgcaat gcttctgtaa caggaaacga gaatgcccga gcgttctcca aggagatttc
    25681 caggcatgga gtaagcatag tgagtggttt agccttggga attgatgctc atgctcattt
    25741 aggttgtctg gaaggaggcg gcaaaacgat agccgtctta ggtaccggaa ttgattgtat
    25801 atatccgagc cggcatttaa aattagctga gcaaattaca gagaatggct tattattaag
    25861 cgaatttcct ttaaaaagtc caccaattgc tggacatttt ccactcagaa atcgtataat
    25921 aagcggtcta tcattatgca ttttggttat cgaggcagca attaaaagcg gttctttaat
    25981 tacagcaaga atggctctgg aacaaaacag agatgtattg gctattcccg gatcgattca
    26041 taacccgtta gccaggggct gccactactt attgcaacag ggtgcgaaat tagtaacttc
    26101 agtggctgat gttcttgatg agctaaaaat tgagcaccat cagttcactt ccaataaacc
    26161 aattttttcc cttgccagcg gtaaggaaaa cctagtaaag ttcattggtt ttgaaactac
    26221 gactatcgat caaatcattg gtcgtagcgg atatggcatg gaacaagtca cgagtggact
    26281 ggctgaacta gaattgaaag gggctgtgat ggcagtgccc ggtggctata taaggtgtga
    26341 atatgaaaga taatttattt gaaatgttat tgaatttgtt tgaaaaaagt cttactcaac
    26401 tccaaaaaag ccataaatcc aatgatcagg actcccaaag tcaattgctt gaagaagatc
    26461 ttttaagtgc tgaagaacaa tcattgttta tcaaatctgc tcaacaaaaa tcaacacgta
    26521 tttttactta cgatgagcaa atgaaactga caaaagccag ttatcaattt ctaatgcgta
    26581 tgaaactatg gaatatcctt gatatcgatc aatttgaatc aataataaat caactacaat
    26641 tctctgaatc tcgtattgta accttgcaag aaacaaaatg gactataaga aatgctctgg
    26701 catcctcctt gaatgaagag caattaactt ttcttgatct tgtgctatac caaacagaag
    26761 atgaattaac aattcactaa ttaatgagtg tactgatatc aacccattca tgaactgtta
    26821 gtgagccaca caacccaaaa aggataaggt ttatttgata tgagtaaaca cttggtgatc
    26881 gtcgagtcgc ccgcgaaagc taaaacaata cagaaatatt taggaaatga ctatgacgtg
    26941 ttagcgtctt atggtcatgt gcgtgatctg cctgctcgta aagggtcagt aaaccctgaa
    27001 aaacattttt ccatgactta tgttcctatt gaaaaaaatg cgagacacat tgatacgata
    27061 gcaaaaactc ttaaaaaatc agactctcta ctgctggcaa ctgaccctga tagagaggga
    27121 gaagctatct cgtggcatat ttttgaatta atgaaagaac gcaacctttt aaaggacaaa
    27181 tcagttcatc gaatcttttt taatgaaatt acaaaagcag caatccagga tgcaattaat
    27241 catcctcgct ccatatccat ggatttggtg aatgctcaac aagcccgacg tgctcttgat
    27301 tatctggttg gctttaacct gtcccctttg ctatggaaaa agattcggcg aggattatcg
    27361 gccggacgag tacaaagccc tgcccttagg ttaattgttg agcgagaaga ggaaattgaa
    27421 cgtttcattg ctcaggaata ctggaaaata atcgcaaaat gcgctcatgc cagcaccgaa
    27481 tttgaagcac gactgaccca ttataacgaa gagaaacttc aacagttttc agtcactcaa
    27541 caagaacaag cccatgaaat aaaaaagcaa ctgatagcac aagcacaagg ctttttaacc
    27601 gttgctcaaa ttgataaaaa acagcgtaaa cgtaagccat ctcccccctt tattacctct
    27661 accttgcaac aagaagccgc cagaaaatta ggtttcaccg cacgaaaaac catgatggtc
    27721 gcccagcaat tatatgaagg tattgatata ggcacaggaa cggtgggttt aatcacttac
    27781 atgcgtactg attcagtcaa cctagcaaag gaagcgattg acgaaatacg cgactatatc
    27841 acacaacgtt ataagggaga taattgccca aattctccca gaatttacaa aactaaatcc
    27901 aaaaacgcac aggaagcaca tgaagccatt aggccaactt ccatcaaaag aactccagaa
    27961 atggtgcaag gttccctgac aagcgatcaa ttgaaactct atagcttaat ctggaaacgc
    28021 acagtggcct cccagatggc tgatgccatc ctggatacgg tgagcgtgga ttttagctgt
    28081 ggtaaaggga ataccttcag agccaatggt tcaacaatag cctttcctgg cttcctttct
    28141 gtctatgaag aaggacgaga tgattctaaa gacgaagaca atgaagataa aattttacct
    28201 gctttcaatg taggtgaaaa aataaaagta tcggatattg aaaccaatca gcattttacc
    28261 gagccacctc cgagatattc tgaagcaact ttggttaaag ctcttgaaga atacgatata
    28321 ggcagaccat ctacttatgc ctctatcatt catactttgc aacagcgcga atacgtggta
    28381 gttgaaaaaa aacgtttttt gcccactgac gtgggtagaa ttgtcaatcg atttctaacc
    28441 aactatttta ctcgctatgt cgactatcag tttaccgctg gccttgaaga cacactggat
    28501 gccatcgcgc gtggagaaaa ggattggatc cctgttctgg aagagttctg gcaacctttc
    28561 gtccagcaaa ttcagaatat agatgaacaa gtgcaaagaa aggatgttac tacggaatta
    28621 ttagaagaaa aatgtcctaa atgtcaaaag ccattgtcca tcaggctcgg caagagagga
    28681 cgctttattg gctgcacagg atatccggat tgcgattaca ctcaggatat aagcaatcca
    28741 gaaggagaaa aatcagaacc tgaagtcgtg gaaggcagaa gctgccccct gtgtcatggc
    28801 gccttgcata ttaaaaccgg tcgctatgga aaatttatcg ggtgcagcaa ttatcctgaa
    28861 tgcaaacata tggaaccttt agaaaaaccc tccgatactg gcgtcacatg ccctaaatgc
    28921 agccaggcga aaattctgca gcggaaatca agaaaaggga aaatctttta ttcctgtgga
    28981 aattacccaa aatgcgatta cgctctgtgg aatgaacctg ttgatttgcc ttgtcctaaa
    29041 tgtgcatggc caatcttaac cttaaaagaa agtaaaaaat ttggtcgtca aattctttgt
    29101 cctcgtgaag gttgtgatta ctctgccaaa gaagactaag tactttgtta ctccagtttt
    29161 tacaccctga gtacctcagg ctattggatt gcgaatgatc tgtttgtttt attcgcaatc
    29221 cattaatcca gcaaattatc tcaactcact aagggattgt tattcttctg taagtataat
    29281 ttggccaaat gataggaagc ataatcggtg atatgtcctg aatcgcgaaa tactggaata
    29341 ccattgatat ctaccttgca catccccgac ggacactgag ctactcgtgg gtcaataatg
    29401 accagtgtct tgtattttct ttgcattttt gcaaacaaat tatcgaacca aatctgttca
    29461 ctgacatcca ggttaaaatc acaccgctcg ggattgtatt ttttatgcag cttgatatgc
    29521 tcaaaaaaac aattgtaagg atttcccttg gacagtgcta tcgatttgat taatacaggt
    29581 ttcgctcctg aagtggtaat aagcttcaga gctttatcca aagccttttc aatgcgcttt
    29641 aaagaaagct cttgagatcg gttgtcattc aattgattaa taattctatc accaaaataa
    29701 ccattccagt tttggcctaa aataacataa tcatagtggt tggttttaat catattaaaa
    29761 tagcgattcg cttgatcatg gcattcctgg taaatcttat ttttaacatt ccaatcaaat
    29821 tgatatatgt caggcaaagc aagacaagca accgttgcat gagccaaaat ggatacattg
    29881 gcctcctttg ccaacgtatc cataaatccc caatgctgat tggaataaga atcgcctatc
    29941 ataaagccgg ttcgactatc cgagtttttt gctcccagaa cgcactgagg attaacttcg
    30001 gtatttttgc gttccagaca caaaggcctc tgtggatttt tgtacttgtt taattccgca
    30061 taaacctgga ttgcttcatt gaaacgatga ggatagcccg cattcttttt aatcagataa
    30121 tcactcaagt gagtgacaaa aaccggcata atcaataaac aaaccactgt atagctgaac
    30181 cggattcgat gcaatttcct tgctggtttt tcaataaatc gccaggaaag ataggcgata
    30241 atcaatatca gcccaaaagt aaccaacaga accaaattgg actcttcaat gtttaaatag
    30301 cgcattaaag ccaatacagg ccaatgccaa atgtacaagg aataggaaat taatcctatg
    30361 aacaccagag gtcttaatga aagagcataa gttactatag attgcgactc ttgttgtccg
    30421 gcggcaatca gtatggccgt tgcaaggcat aaaattaaag tataccaatt gggaaaacct
    30481 atattaatgt catgatgcat tgcaatataa aacagcgtca gaacagccaa gacagttata
    30541 aaatttaata catacttatt ggctaaaaag cgtttactcc ctgaagctga ggcaacacag
    30601 gaaccaatta aaaactcaaa gatacgactg gaaaattgat aatacgtttt tgcaggatta
    30661 ctccaagaat aatacatgga taatgcgaaa aaagtgatgg ttaataaata ggttatcttg
    30721 attcgatgct gtggaccgac caatcgataa agcaaataga ttccaaccgg caagataaaa
    30781 taacattgcc attctacgga taaagaccac atatgcaaaa gaggcaattg attgctatcc
    30841 ggagaaaaat aatcgctcgt gacattttta aaaaacacat tcgatgtaaa aatagatgtt
    30901 ttacgcgcac ttttactaaa aagcatcaag tcatcgggca gataataaaa cagggtaacc
    30961 aaggttgtaa ctacaatcaa acaaataaaa acaggctgta atctccataa tcgcctgctg
    31021 taaaagtcca taaaagaaaa acgattattt tgcaaagatt tttcaatgat acttgtaatg
    31081 agaaatcctg aaatgacaaa gaaaacatca actccaataa acccggatgg gaaaagagtt
    31141 aagcctccat gaaaaaataa aacaaataaa atagcaattg ctcttaagcc atcaatatct
    31201 gagcgatagt tcacactaca atttccataa aatgattgtt gattaatact aattacatgc
    31261 ccacccttgc actttatttc tgtacaaagg tttgcaaatt gggaaattca gagttatatc
    31321 acaataatct ttttttagtg agaattattt tagtgtggtt cattcatcaa ttgttcaatg
    31381 tagcgtctgc aggttaacgg tttatataaa taattaatta aatagcgagt tccaccagtt
    31441 cctgtgatta tcaaagtacc ataacttaaa atactgccta aaatggattg acgtatgtct
    31501 atcgtttcaa ttttattcag ggggatatcc acagtttgcc ttactatgat accggtacgc
    31561 aaaataactt gcttcctttt tatggtaatg gaagaaaaat gataggttac ccaggtaatc
    31621 aatatccaga tcagtgcaaa aaatcctaaa aaaagactca cttcgcgcaa ctggatgata
    31681 taaatataaa tcgccatcac taaacccaat ccgacgaaag gaccaaaaaa tatgatccaa
    31741 tgcaatctgg taaaataaat gacattactt tctgtagtat tttcagccat tgatcaccct
    31801 tatatacctc aaaaaaatat gatattgcac ttaacgttaa tttgctattc tatacataaa
    31861 tcaacttatt tttcgatagt ctaactcttt aatagatagc taacaattaa gacggacgtt
    31921 atgaaatcaa tcagtcaatg cctaaacaag caactcagta caatttgcca gcattctatg
    31981 caattggaag agttatctct taaaatttct gcatttcttc cttcccattt catttctaaa
    32041 tgtcaagtag gtagttttaa taaagggtgt ttggttttaa ccacaaccaa tgctgctttt
    32101 gcgtccgaaa tacgttattt acttcctgaa ttacgggata aattgagaaa agaagccggc
    32161 ctttatcagc tgtcttcaat taaaattacc atcattgctc cctcctctca aatcgaaaac
    32221 cctgcacaaa aaaaatctct taccctgtct gaaaaagcca aggcaaccat tattagtgaa
    32281 agccaacact gtacttatga gccactgaaa aacgcattac tgcatttagc tcaaagtgaa
    32341 taactcatca accatctcat ttgtattatg aatttttttt cactataatg ctcaaatatt
    32401 aaactaatat gttagagctt gctatgccta aaataaataa aattgttaat ggaaaagacc
    32461 taactcccca ttatctgagt gagccaaaca aagaatttaa aatctatcgt tataataagg
    32521 aagtttatgc tgttcgtttt gagaattatg aacccataga ttatgtttta atgtggaaaa
    32581 gtcacaagag tgagcatacg cagaacagtg aaatgattaa aaatactgaa gaggattata
    32641 aggagcttgg aaaaggagaa caaggagcgg tatatgagaa aacagaagat aaggctatga
    32701 aagtatctcg aggcagacat ccaagagaat tttatgaaga aattaacctc catattatag
    32761 aacagcaatt tttccttaaa tatcatggta ttcaagagca ctttgttcta ggactctgga
    32821 gtatcaaaaa tgaagaaaac gtgtatttct acatgcctaa aatcaatgcg atccctatta
    32881 acaaaaaaat cgaccagccc aaaattgagg aatttgttct tgcgcttaaa gaacttaatg
    32941 atgcagggta ctggcatcct gatttggcaa ataatcctta ccatattagt ccacaaaatc
    33001 tgattgcaac agaagaaatg gtaaagacta ttgatcttga tggtggcttt cggtacgaca
    33061 agggaagagt tgatgaactc agcaggaagt ctcttgttta tggtaaagat caatggctat
    33121 atgtctataa tttcatctat ccacccaccg atgaggagga taacagaatt gattggagag
    33181 tccctataga gaaatggtat gaaaataaca gagacgaatc tttatcagat aatccacatg
    33241 cgttgcttcg attttaccat gagggactta tatcattgcc aaaaaaactt gcccatgatc
    33301 tgcatgagac aatattagaa gagttatccc aggataaaaa atttaaaaaa cattcgcacg
    33361 aggtaggagt aaatcgcttt tttagcccga aaaaacaaga aatctatgag ggtttaaagg
    33421 gtgatgggct aaaaaaagtg atcctgaaag aacttagaga ctctttagct gagataagaa
    33481 caatggagca attggaggaa aaaaaactgg agtttttagc ttccccagaa atgcaaattc
    33541 ttgctgaagg tcaggataag acaacaaaag cactgaattt aaaaaccagt tctcgtaaaa
    33601 aggtgatggt aatatttaaa gaagcagaag agcgaatatt gaatagccct aatgtaagca
    33661 tttaggacgt gtcaatttta attctgtgaa tagcatgctt ttatgtgagt tagtttcggc
    33721 ttaattcaaa taactcttga aaaattaatt aagccttttt catggcaaac ttatagcgtt
    33781 ttgacaattt ttttaagtaa agctccattg ataacttccc cataaaaatg ggccctcact
    33841 attttctttt ttcgctaaca tttcttttga ggcaattcgt ccatcatcga tattggcttc
    33901 caatcgatta aggtgcatta taatcaaatc aatacaatgt atttaaatcc ttcttaaggt
    33961 gatcatgctg aaacaaattg tgctaaccgg tctagttatt atagggattg tcattacgct
    34021 aatgtactta tttcagcgcc acctcattta tttccctaat agacataccc caaagttgga
    34081 agattacaat gcatcggata tgaaagtaat ttccttacgt acaaaagata atttacattt
    34141 aaaatcctgg tataaacctg caagcaagca tcgccctaca atactctatt tgcatgggaa
    34201 tgctgggcat attggttaca gaatgccttt ggttcgtgaa tttattgacg caggtttagg
    34261 cgtattttta ttagaatatc gtggttatgg aggcaatcca ggcaaaccta gcgagaaagg
    34321 tctttatgca gatggagaaa cagccattga atttttaatt caacatggtg tgtcatcaaa
    34381 gcgcgtgatt ctatatgggg aatccatagg taccggagta gcaactcatc ttgccacaaa
    34441 atacccggtt tgtgcggtta tacttcaatc accctttaca tcactaacca ggcttgccca
    34501 gtatcactac ccattgaact tccttaaacc ctgggatcaa tataattcac ttgcccgtat
    34561 gaaaaaaatt aatgccccca ttctggtttt gcatggaaaa ttggatcaga tagtaccgta
    34621 tcaagaaggc ttaaatgttt ttaatgaagc caatgaacct aaaaaaatga tttcttttga
    34681 cgataaagag cataatgatt tgtggagcgc tgataatttt tcgagggaaa ttattcaatt
    34741 tgctttaaac cagtgttcct agcttttttg gatagttcat tgttaaagca ttttcatcat
    34801 caacgttatt ttgaacttaa tcatccattt ccgcaaaggc cattgaaaat ctcctttcca
    34861 cccactcctt attgaggtac tgaaaacaat cttgcagata atcggccaat tcctcttcat
    34921 ccaagacatg ctcaaaacct acaggttcaa cgtcatggtg aaagtattcg ccgaagtact
    34981 tcattgagaa atcaatataa tctcgagtat gcaaaataaa agaatgccac atctcatcga
    35041 ggatcaacaa tggtccaaaa agataggttt ttttcccccg caaccttctt tgcttattaa
    35101 gccacatcca ggctaataaa tcctcaaaga gaaatagccc ttcttgtatg gaatattctg
    35161 gatgagaata gcaaaaatga gtcactattt tttcattttt atagtgcagt aattcagaaa
    35221 gagaaaaact cattaacgtc cacaacaagt ttttttatta cttggtttct gagtaatgaa
    35281 tctaattatt ctacaaatca tttcttcttt agccataatt gtctcttatt gttaaaataa
    35341 ttcaagttta acccaatgtt tgtaaagggc gcaactgaat gtttttattt tcatcaatca
    35401 gtatcatatg ttgtggcggc acatcttccc atccttcatc caaatcattt agcctttcag
    35461 aagagactac aacataagct ggattacctt cttcttttat ccataatcct tgagtagata
    35521 atacatagcc aagcaaataa tgcagcgtca aaggtttttt cttctcatga ctgcaatagc
    35581 gggtagcgat aatcttttta ccatttgtaa gacaaatatt aaaataggaa ggtccactat
    35641 ggccaaattg tcctattact tccaatattt tgttaatagt ttcttgcagc acatcagcag
    35701 ccaccgatag ttgattaatg tctcttcctt ttgctaattg caaaaacaaa ccaaacaaat
    35761 gctcagaatc tgtttgacct ttaacccaat ggtaaatgtc atcatccaac aaatgcctta
    35821 tatgcctttt cacatcaata aaatcattaa tttccccatt atgcattaac atccaatcgc
    35881 cataaataaa gggatggcag ttataattgg ttactcctcc tgcactggca gagcggacat
    35941 gagcaaaaaa acaaggggac tgaatttttg ctgttaagtg taaaagattt ctatcgttcc
    36001 aggctggata aacggaggtg aacaaagcag gatcagagct aacttctggt gaataccatc
    36061 ccaaaccaaa accatctcca ttggttcgat atttggtttc gcgtgcatgc agactctgca
    36121 tgatgattga attcttaggt cttaccaaca cattttccaa tagcgtaggg cgtcccaaat
    36181 aagcaacgaa tcgacacata acagcctccc tcatcgagtg attcgaatag caaactctaa
    36241 ttaattaact aacttccaac tttctattac tcatatccct aaatctgtaa tccattcggc
    36301 tgtcattgcg cgtttttata aggtacccgg aatcactgca aatcaactca ctttaccata
    36361 tgatgcaacg attcttactt atcatagggc taccatttgg aatattaaat gcctcttgaa
    36421 attgaggaat atttgccaag ctaccattga ctctgtatac agcaggcgga tgtggatctg
    36481 ttgtcacttg attacgaagt tgctccggcc tgacattcat agcccaaaca tgagctgttc
    36541 ctaagaaaaa ttgttgatca ggggtaattc cttctatcgt tttagcattc ttatactctt
    36601 ttgagcgctg aaacgcttta tatgccaaga tcattccgcc cagatctgca gttgcttcgc
    36661 caacaaccaa ttgtccctgt acatgcagat cgccatctac tacatatttt gagaattgat
    36721 cagcaatgca ttgcgtcgca tttttgaatt ttgataaatc atttggcgcc caccaatttt
    36781 tcaaatttcc ataaccatca aattgagcac cttgatcatc aaatccatgc gtcatttcat
    36841 gacctataac atatcctatt gctccataat ttatggcagt cggagccttt ggatcaaaaa
    36901 agggagattg taatatacct gcgggtatat tgaggttatt cattgaagga tcataatacg
    36961 cgttgatcgt ttgaggcgtc atcgcccatt cacttctatc tacaggttta ccaattttgt
    37021 cgagatctcg attgatcaag aattcgtttg ctctgataac attcaatacg taggggccgc
    37081 gatctatttt caagctggaa taatcccacc atttggcggg gtaaccaact cgttcttcca
    37141 ttaaatccag ctttttcaat gccgcatcac gagtcttggg tgacatccag cttaaagttt
    37201 ttatatcttc ttgtagtact gctctgatat tttttagtat atccaagacc ttttgtttgg
    37261 attcaggaga aaaatatttc tctacataca attctccgat tgcaaatccc aatgcaccgt
    37321 tttcagtgtt aactactctt ttccaccttg gtaatagttt ttgtgtacca gttaatgcag
    37381 aaaccatctt aaaattctga tcgacaaacg gtttggataa ataaggagcg aatgcatcta
    37441 tcaaatgcca acgcagataa attttccatt catccaaact aactgactgt aataattcat
    37501 tcatcgcttt gaaaaaatca ggcatagcaa aattgatttt tttaattttg ccctgacctc
    37561 tagcgctcaa gtattgagac caggaaaaat taggtgtcaa tttatccaat tcagtaatgg
    37621 tcatcatatg ataaatggcg tgtggatctc gttgctccac ttgtgacatg gacgctttag
    37681 ccaattgagt ttcaatatcc attacaattt tagcttcttt tgccgccatt tcaggactgt
    37741 cacccaacag ctcaaacatt ttagtaagat ggttaacata agcttctctt acctgtttaa
    37801 atttagcttc ttttttaaga taataatctc tatctgggag tcccaatccc ccttgcataa
    37861 cagcacctat catggaagtg ctgtccttaa aatcttgcat gctgccaaaa ttaaacaagg
    37921 gccctacgcc tattttgtgc agatgggcaa tttcagcctg taaatctttg agattcttaa
    37981 ttgcttctat tttagtgaat tctggtttta gaggcgtgat acctgactta ttaatgctat
    38041 caacatccat accgctaaaa taaaaatccc ccactttctg ctcaatactt ccaggtttag
    38101 catgagtatt cttggatgca ctgattaaca tctgatgaat aatgttttgt actttctcat
    38161 ttacaacata aaaacttccc caggaagcat agtcagcggg tattgggtta ttctttttcc
    38221 aattgccatt ggcataagta taaaaatcca cagctggtga cacactggta tcacgccagt
    38281 caagatgaag aagatcgttt gtattattat cggaaaaatt ggagttggaa gaaaatacta
    38341 taaaactaga taacatcgta acaatacaag ttgcaatctt aaatttcatc ctgatattcc
    38401 ccttaatttg agctttatat aaatatctaa agaaggaaac tgcttaggcc attatattta
    38461 gtatagataa tgcctataaa atgataataa gtgaaaatga atattctcac tacgtggata
    38521 tgatgtattt ctgtcaggct tcaacaggat aatattcagg gtgaaccgcg ggcagataat
    38581 tttctgtatc aaaataggta tattcccaag catcctgcct taccattaat tcacgcaaca
    38641 atctgttgtt caattcatga ccagatttat atccttcaaa tgcgccaatt aaactggaac
    38701 ccaataaata aagatcaccg atagcatcta atactttatg agtaacaaat tctgattcga
    38761 agcggagacc atcttcatta aggacacgat aatcatctac tacaatagca ttatctagac
    38821 taccgccttt tgccaggtcg cattctctca atttttcata atctgaaaga aaaccaaaag
    38881 ttctcgctct acaaacttct ttgacatacg atgttccaga aaaatcaaag cttactgttt
    38941 gtggtctatc attaaaaacc gggtgctcaa aacctatggt aaatgttatt ttatatcctt
    39001 tatgaggaag gaactgaaca tactttccat tttcttcgac tcgtataggt ttaagaattc
    39061 ggatatatct ttttgctgca ttttgctctc ttatacccgc tgattgaatt aaaaatacaa
    39121 atggggcggc acttccatcc ataattggaa tctctggagc attgacatct atatatgcgt
    39181 tatcaattcc taacccggcc agggcggata ataaatgctc caccgttgca attttcacct
    39241 tgccatgatg aagggtggtg caaagcatgg tatctccgac gtattcgtag gaggcaggta
    39301 tttcgacaac tggtgacaaa tccacgcgtc taaaaacgat acctgtgttt acaggagctg
    39361 gtcttaaggt taaaagcact ttctcaccgg aatgtaatcc aacaccagta gcctggatta
    39421 cctttttagg agttctttgt tttatcattc agtgctcacc tttctctctt atatttattt
    39481 tgcacgcaaa aaccttaaga tcttagcaac aatttattga ttagtctaat ttttttaatt
    39541 tcaattaatg cggttttgga aaccaaatac aaaaattgca aaaaataacc acacgaatca
    39601 attcatgaga tactcaagaa agaacgtttg tatccatcaa atacatcctt gttttcaaac
    39661 acatcctctc tcaagatatc ccgttaaaaa tgcttctatg cttcttcttg cctacgtaaa
    39721 aacgccggta tgtccaggta atccacatca ggaacactct cattaccatt attggtttgc
    39781 tttgtagctg ttgttgcagt accagtctga gcctgcttac gaatcaccgc cggtctctcc
    39841 agttgttgat aatcaagaga cccatcgctt cttgttgttt caaccaggcg tgctctaaga
    39901 ggttgttgtg gctgttgctg acgctgtcta ttatcaccta aaccagtcac aatcacagtc
    39961 actctcatct cgtctgtcat ttcaggatca ataacagttc caacaactac agtggcatca
    40021 tcagaaatga attctttaac cacgtcaccc acttcttcaa attcaccaat agacatatca
    40081 aggcctgcgg tgatattcac taatattccg cgagcgccgg agaaattcac atcctccaat
    40141 aaaggagatg caatcgctgc ttcggccgct tgacgtgccc tttgctcacc aactgcgctt
    40201 cctgttccca tcatggccat gcccatttct gacatcaccg tacgcacatc cgcaaaatca
    40261 acgttgatca aaccagggcg agtaattaaa tcagaaatac ctttcacagc accaagcaac
    40321 acattatttg cagctttgaa cgcattgagc aaactgatat tttttcctaa aacacttaat
    40381 aatttattat taggaatagt aattaacgaa tctacgtgct cagccaagcg tcgaatacct
    40441 tcctcagcgg caagcgccct ttgttttcct tcaaaagaaa atggtttagt taccacagcg
    40501 accgttaaaa tacctaattc cttagcaatt tcagcaaaaa caggcgcagc tcctgtccct
    40561 gttcctccgc ccattccggc tgtaataaag accatatctg caccactaag aatctcttta
    40621 atgtgctctc tgtcttcttc tgctgcttct cttccaattt gtggattagc acctgccccc
    40681 aagcccttgg tcaatgcatc acccaattga atgtgtattt tggcactgga acctcttaaa
    40741 gcttgtgcat cggtattcgc acaaataaac tccacaccat caatattttc agcgaccata
    40801 tgttcgacag cgttaccacc gccaccacca acacctacaa ccttgatgac agcattattt
    40861 tcttgctggc tgctttccat taattcaaac ataaccctgt ctcccctaat gtgctgcttt
    40921 tttcttatta aataaattaa acagttttga acttatcgca tttgacactt tacaagacaa
    40981 aaatctgcat ttgttgtcat gcaaaatatc atgcaacccc atatctcaat ttccatccat
    41041 accaggaaca tttttaagca tcatatcagt gtttttacat gaaaagctaa aaatttcctt
    41101 gaaaccattc tttcatgcgt gaccaaatac ttttaccatt atcattcatt gctggcacat
    41161 tatagccact ttcatattgc tgttgaaagc catgaagcag taaaccaacc cctgttgcaa
    41221 acgaaggatt ttctgtcgct tcagtcaaac cagagacata atgcgcacat ccttttctga
    41281 ctggcatttc aaaacaaagt tcggccaact caattgctcc tcttacgttg gaagctccac
    41341 ctgttagaac aatacctgca gccaaacggt cttcaaaccc gcttcgacgt aattcattac
    41401 gtaccagtga aaatagttcc tcgtatcgag ctgaaacgac atctgccaaa gctttggccg
    41461 agatttttcg cccaggccta tcattgacac tggtaacttc taacatttgg tttggattgg
    41521 ctaattctgg taatgcacaa gcatgattga ccttaattgc ttctgccgcc ttggttggcg
    41581 tacgcaaggc cattgcaata tcatttgtca cttgatcacc tgcaatcggt atgactgaag
    41641 tatgctggat agctccctca caaaatatcg cgatgtcggt agtgccgcca cctatgtcaa
    41701 ttaaacaaac cccaagctct ttttcatcct cagtaagaac agcatgactt gaagctaatt
    41761 gctccagaat aatatcaatc acatccaacc cgcagcgcct gacacactta acaatatttt
    41821 gggcagcact gacagaacca gtaacaatat gaacccgaga ttccagcctt acaccagcca
    41881 tacctatcgg ttcacgaata ctgccttgat gatcaatgat aaattcttgc ggtaatatat
    41941 gaagtatctt ttgatcagcg gggattgcca ctgcctttgc tgcatcgata accctttcca
    42001 catcggcttg agatacttcc tgatcacgga tggccacaat accatgggaa ttgaggctac
    42061 gtatatggct gccagcaatg cctgcgtaaa cagtcctcac ctcacaacca gccattaatt
    42121 cggcttcttg tacagcacgt tggattgaat tgactgtagt ttctatatca actacaactc
    42181 ctcgctttaa tccgcgagag ggatgacggc caatacctat aatttctatc gttccatctg
    42241 atgttacttc accaattaat gcaataattt ttgaagttcc aatatccaac cctgtgataa
    42301 tatttttttc tattttttta gccattatcg tcccatttgt tgtttccact gcactgccat
    42361 gccacgtgga taacgcaggt ccacgccagc caactgatct gctttttcag caaaaacagc
    42421 agggtatgct ttacaaaaac gcagcaaccg ctcttctaac tctttctttc ccaagtatat
    42481 ttttacacta tgattaagca gcaacaccca ggattgatta tctcttaaat acaagcctga
    42541 agcatttaaa ccgtatgatg ataatatctt actcaatttt tcataaactt gtaagacttc
    42601 taattgctga gattgcggac cctttaattt gggaatatcc aaatcagatg gaagagaacc
    42661 ctgattgaat aactttccat cccttgtcat tagagcatca ccccaaatcg ctacgggctt
    42721 tttttcagtg agtttaattt tcaatgtatc tggccaaact ctttctacat aggccgtatc
    42781 aatccaactc attgaattca actcactttg caatcctttt actgataata aaaagaaact
    42841 ggcatccaga tatttagcta acacattctc caattctttg tgtgttatat gttcatatgt
    42901 cgctgcaact ttgatcgttg taattggaaa acgctctgca tcagcaagat ataaatagcc
    42961 taagcgaccc gctagcagca aagcactaag aattaacaaa cccattatcg ttaaataacg
    43021 caagccgaca gagttcactt ccataattta gtgttccctt cacaagtaat ttgtcaccaa
    43081 atgttgacga cagttatgat gaaccattgc tagattaatc aattcatcaa gtaaatcagg
    43141 ataagctaca ccagttgcct gccacaattt ggggtacata cttatggagg taaatccagg
    43201 taatgtattt atttcattaa agtaaatttc ttctgtctta tcatttacaa aaaaatctac
    43261 cctagccatg cctttgcatt taagataact aaaaatattc gctgcagcct gttttaattg
    43321 ttcttccaga ctattattta attgagctgg aataatcaaa tccgtttgac tgctttccaa
    43381 atatttcgca gtataagaat aataaccgtc agggtggttt acttttatct cacccaccat
    43441 acttactctt ggtttcccac agggaatact gttctccaac actgccagtt caatttccct
    43501 tccgacaata aattcttcaa ccagaatttc ttcatcatag cgtaatgcat cagcaactgc
    43561 tgcgtttagc tcatccatat tatttgcttt gtgaattccc acactggaac ctagtgagca
    43621 tggtttaacg aacagaggcc aaccaaactc cgaggcaact tcatgacaaa attgttgtct
    43681 ttcagacgca ttggcatgcc atgataataa tttatatctt gctgatttga gcccattaat
    43741 gcatgcgagt cgcctcgcca tgtctttgtc cattccgatt gcagaagaca atacgtcaca
    43801 tcctacatag gcgacaccgg ctaactcaag taaaccttgt aaacatccgt cctcatataa
    43861 agggccatga accactggaa aaacaatttc agcatcaact gccagacgtc cattaattaa
    43921 taagccttcc aatggggttg atttttcagt aacaacaggt aatttatcag aacaggcgag
    43981 caaatcatta taatcatgac gatgaaattg accactttta tccattgcaa tgggtataat
    44041 atgatatttc tcggaatcca ggtgttttag aaccgatgcg gcagaaacca atgacacttc
    44101 atgctcgcct gattttccac cataaagtaa aaccaaatta acgggtttgg acattagcgt
    44161 ctccaatgat atgtacttcg cgtatcaact ctatagatgt ttgctcacga actttagttt
    44221 gcaccaaatg aatcaaggct tcaatattag ctgccgtggc agtaccttga tgattgataa
    44281 taaaattggc atgtttttca gaaacaactg ccccaccaat gcttaccccc ttgaggccac
    44341 aagattcaat aagtcgcgct gcaaaatctc caggagggtt tctaaatacc gacccacaat
    44401 tatattcatt agttggttgg gtcttggctc tatgagccaa taaatcctta attaattgca
    44461 aggatgtctc tttattacct ggtgatagct gaagcttggc ggaaataaac cattcatctc
    44521 ccaagccagc aacatgtcga taagctactt caaattcttc cggtttacgg gttctgattt
    44581 cacctcgacg atttatcgtt tgtacttcaa taaccgactg ccaggtttca cctccatgac
    44641 aaccggcatt cattcgcaga gcaccaccca tggtaccggg gataccagcc caaaactctc
    44701 ctcctgacag attattacgc gcagaaaaac gagccatact ggcacaggat acccctgcct
    44761 caacccgaat acagttatcg ctcaataggg tcatttcttt taaacagccc tgagtgagaa
    44821 tcacagtacc ggaaaaaccg ccatcgcgaa ttagagaatt agagcctaaa cctaaccata
    44881 ataatggctc atcaaaaggc aatcgactta aaaacaaagc caaatcatct atatttgctg
    44941 gtttatagag cctcgctgca gggccaccaa ctcgccaggt agtatactcg gctaaaggct
    45001 cgttgaaaag caaagtgccc tgagactctg ttacatgacc gctgtccatt cccgttatgc
    45061 tcatgttcat tctcacatgt ttttcatcag gttcactgcc atttggccga tacttccagc
    45121 tccctgcatc aaaataacgt caccatcttt aataaattca tctaaggtgg ctttcaacga
    45181 ttgctcgctc actatggtca ctcgtttatc cctggatcgt atttcattgg ccagattttc
    45241 actagtaacg cctggaatag ctgtttctcc cgctgcatag atatccatca ataacaactc
    45301 atccgataaa cttaaaacat cgacaaattg tctatgcaag gactgtgttc tggtataacg
    45361 atggggttga aagacatgaa ccaaacgtct ctcgggccaa acacgtctaa aagcatcaat
    45421 tgtggataaa atttcctgtg gatgatgtcc ataatcatcc acaataatcg ccgcgccttt
    45481 ttcgaactgt ttttcaccaa gcatttgaaa gcggcgtccc accccttgga atttttgtaa
    45541 gccacgaaca atggaatcgt catcaactcc caactcggtg gcaattgcta tagaagctaa
    45601 tgcatttaat acattgtgac ggcctggata ttggaattgt atagtcagtt gtttatgtgg
    45661 tgcaggtcga acgactacaa actcactcag catgcccttt tgagtccaat taattgcccg
    45721 ataatgagct tcttctttaa acccataagt taaggttggt ctttgaatag ccgggaggat
    45781 acggcaaatt tcttcatctt ccaaacaaac tactgctaat ccataaaaag gcaaatgatg
    45841 caaaaattcc aaaaatgttg ttcttaattt ttcaaaatca ccttcataag tatccatgtg
    45901 atccgcatca atattagtca ctaccgccat cataggcttc aaaaacaaaa acgaagcgtc
    45961 gctctcatca gcctctacaa caaaataagc tgactttcca agctgagcgt tcgcaccaca
    46021 actattcagc tttccgccaa taacaaaact gggatccaaa cctccttcgg caagcaaact
    46081 actgaccaag ctcgttgttg tcgttttgcc atgagtgccg gcaattgcta taccatgcct
    46141 aaatcgcatt agctcagcaa gcatagctgc tctaggaata actggaatca ttaactcacg
    46201 agcagccaca atttcgggat tattaaaatc aacggccgaa gaccttacta caacatcagc
    46261 gcctttaatg ttttccagcc tgtggccgat atagacttga atacctaatg accttaagcg
    46321 ttgtaccgtg ccactttctc cgagatccga accagtaata cgatagccct ggttatgcaa
    46381 tacttcggca ataccacaca taccagcacc accaatgcca acaaaatgaa tttgctctac
    46441 acgccccatg cgcggagaca aaaattgctc tgagttattc aatattcccc cttaagccgt
    46501 tagcggtctc aacccaagag actgccaacg attttcatga tctatgcgca ataatagtgc
    46561 aataacgata caattaatta ccatactggc cccaccataa ctgagtaaag gcagagttag
    46621 ccctttggtt ggtaacagtc cggcattaac acccatatta atagaagcct gtaaagctaa
    46681 ccaaatagtc aaaccatagg ctgtatagga tgcaaaatgc ctttcttgag tataggcggt
    46741 atatcctatg tttaatcctc taataaccag tatactatac aatgtgatga caactaaaat
    46801 tccaaataac cctaattctt cagcaatcac agcaaataaa aaatcagtat gcgcctcagg
    46861 taaatagagt aatttctgta tgctttcacc taatccggta ccaaaccatc cacctcgtcc
    46921 aaaagcaatc aatgactgag tcaattgata accactattg tattgatctg cccacggatc
    46981 caaaaaggct gttaagcgag caaccctata gggggaagat accgccaaca gagcaagtgc
    47041 tgtcactaca accagcatta aaccaaaata gtatcttaat ttaactccag ctaaaaataa
    47101 catagccatt actgtaccag aaatgacaac tgtcgctcca aaatctggtt ccagcagtaa
    47161 taaaactgaa acaacagcca gtattgccat gggtttaatg aatccaaaaa tgctttcaca
    47221 aaccgcctct tgctggcgaa ccaaataccc ggaaagatag aatatcattg caagtttcgt
    47281 taattctgag acttgcacac caatcggacc taaagctaac caacgccgac tgccatttac
    47341 tgatttacca atacccggga ttaaaacaat gagcaataaa aacacgcaac caatcatcat
    47401 gggcatgctt attttttccc agaaactgct gtctgtacgc accacaatta aggccagcaa
    47461 gagtcccaca aataaataac aagcctggcg aataagaaaa tgaaaaggtt gatgaaagta
    47521 ctttgttgaa atcataaccg agcttgatgc caccatcatc aaaccaatta tcagcaaacc
    47581 aaaaactgcc cctatcagcc atttatcata aagtgatatc ggtctgctaa cgggtttgcc
    47641 tctttgattt aaatgtctcg gtcgcatcgt gttcacaatc cgcgcactga agatgtaaac
    47701 acatcgcccc tatgattaaa atcacggaac atatccaaac tggcacaagc tggggataac
    47761 aaaacgacat caccaggttt cgcacatgtc ttggcaatag tcacagcgcc ctccagagaa
    47821 gaagcacgca cgacaggcac tactttggct aaagccgatt caatcttgtc agcatcttca
    47881 ccaatcaaaa caatggaacg aacaaattct gaaactggct gggctaattc ttggaaatcc
    47941 gccccttttc cctgtccacc cgcaataaga acaattttcc cttgcataga acctcctatt
    48001 ccattaatag cagagattgt agcaccaata tttgttcctt tcgaatcatt aatccaacct
    48061 actccatcca cttctcttac ccactggcat cgatgtggca gtccagggaa ggtttttaaa
    48121 acattaagaa tgtgttgcat agagattccc gctgcttcag ctaaagcaca agcagcaagc
    48181 gcattcatcc aattatgcac cccttttatt aaaatcgatt ccacaggcaa aagacgctcc
    48241 attcctttcg ccaaataagt agtattctct tgttcaatca gcccccaatt tcccattgaa
    48301 ggcgcgtctt tgccaaaaga gatgcatttt atatctgctt gacaggattg gtgaggaaca
    48361 gtgtagacgt cttctcgatt aaataaaaca gccttggcac cacgataaat cctttgtttt
    48421 gcttgcgtat aggcttccat agtgtgatgc ctgtccaaat gatcaggcgt tacattaaga
    48481 atcgtcgcaa ccactggcga caaagaataa gttaaatcca attgaaaact ggataactcc
    48541 aatacccaca aatcgtagtg atgttcatca tccaacatat ctaagacagg agtgccgata
    48601 tttccggcta cagctacgcg aaacccggcc gccttagcca tttctcccac caaagtcgtt
    48661 acagtggatt tgccattggt tcccgttatg gcaatgacag gggcgctaat ctctctggcc
    48721 aggcattcta tgtctccata aattgatgcg cccgcttgac gcgctctctc taaaacagga
    48781 gtatccagtg ccaaaccggg gctagtgata acatccgtca cctggctgat tacttcatca
    48841 ggagtctgtt gcaggtaaat tggcacatca ggaaattcat tttggaattc agctaatcca
    48901 ggagcctctt tacgtgtatc aaaaacaaca aacgatttat tatttttctt caggtatctt
    48961 gctatagaga gtcccgtctt acccagccct gctaccaaat ataaggaatg attcatgaat
    49021 gcaccttgtc tatcgaagtt ttaaagtagc caatccgcat aacacaaata caaccgtaat
    49081 aatccaaaac cttactatca ctttgggctc tgaccacccc tttaattcaa aatggtgatg
    49141 taatggggcc atcctgaata atctttttcc gcctgaatat ttaaaataac ccacttgtaa
    49201 tatgacagaa agcgtctcaa ttacaaataa tccacccata atgagcagaa caagttcttg
    49261 cctaactaca accgcaacta tccctaaagc tgctcctaac gccaaagagc ccacatcgcc
    49321 catgaaaact tgggcaggat aactgttata ccaaagaaaa cccaggccgg caccaacaat
    49381 agaagaacaa aaaatcgtta gctcaccagt attgggcaca taagggattc ctaaataatt
    49441 agagtatacg gcgttactcg atgcataggc aaacacaccc aaggcaccag ccaccattac
    49501 tattggcata atagccaagc catccagccc atcagttaaa ttcactgcat tactgcttcc
    49561 aacaatgacg aaataggcaa gtacaggaaa aaaaacgccc agatcccaag tgaccgtttt
    49621 aaaaaaagga actgtgagct gggtatgtac tggcaaactg gcattccaat acaaataaga
    49681 gacagcaact aaagcaatga ctgactgcca aaaatatttc caacgaccag gtaatccttt
    49741 gctatttttt aataccaact tgcgataatc atcaacccaa ccaaccaaac cattagccaa
    49801 ggtaaccaag agcaccagcc acaggcttgt ttgtcttaaa tcgcaccaga gcaagcaact
    49861 cacagtgata gccaataaaa tcaaaacgcc ccccatagtg ggagttcccg cctttgataa
    49921 atgggtttgg ggaccatcac tcctaaccat ttgacctatt tgcagccctc ttaaccagcg
    49981 gatcattaat gggccacata acaaacccac tataagggca gttaatgagg ctaaaataga
    50041 cctgaatgtc aaatattgaa aaactctaaa cgcatgatat tgtccctgca ataactgcgt
    50101 taaccaatag agcataatta ttcctcttgt tcttaaaatt atgctcaatg acttttattg
    50161 agcataattc ctttaatggg gcaaaaaagt ggccaagcat accacattcc tgtcgattca
    50221 atgaagaggt tagccctcta cctgaaccaa ccggtgcaca acattttcca tagcactgga
    50281 cctggatcct tttaccaata ccgtagtatc aggtgttaaa tcatttttta ccacttcaat
    50341 caactcttct tgactgcaaa aatgctggcc tccctcacca aacgcctggg atgccagttc
    50401 actatgtaaa ccgcaactca tcaacttatc tattcccagc tggcgagccg ctatgccaac
    50461 ttctctgtga tgttgtgtcg tccattcacc caactctccc atatcaccga atacaaaaat
    50521 ttttcttcct ggacgcccag ataaaacttc cagcgctgtt aagacagagc gtaaattagc
    50581 attgtatgta tcatcaataa ctgttgattg attttgccct gacaacatcg tcatccgacc
    50641 ttttacacca ccaaaatgag ccagtcctct ttgaatgtca ttgcctgata ttcctacagc
    50701 ataacaacaa gctgcggcag ccaaagcatt tcggacattg tgcaaacctg gtacttgtaa
    50761 ctcaatctcc actctgctat taggtaaaac caatgtaaaa tgagctcgcc catgggtatc
    50821 caagcgtata tcctgggcgt aaatatctgc aggatgaagt gaggaaaaac gtaatatttt
    50881 tttatcagca atcaaatcat cccaaaagtg agcgtaggca tcatcatcat tgactatagc
    50941 aacaccagtc ggggataagc cttgatggat ttcacctttg gaacgagcga ctccgtctat
    51001 tgatccgaag ccttcgacat gtgccggggc aatattatta attaaagaca catcaggctg
    51061 cactatccct accgtatgcg ctatttctcc gggatgattg gctcccaact caaaaacagc
    51121 atagcggtgc tgcttattta aagctaacat gcttaaagga acccctatgt gattatttaa
    51181 attgcctttc gtagcgtgtg atggctgcgg taaaatagtc gctatcatct ctttgacagt
    51241 ggtttttcca tttgaccctg ttaaagcaat aatagggcaa ctcatttcct gtcgatgtgc
    51301 cgcagcaatt ttggccaaag ccatcaaagg actgacaacc acaaattgag gaatatgaac
    51361 gccgtcaatc gatcgattaa ctacagcagc tgaagcgcct ttagcctcgg cttctttaat
    51421 aaaatcatga ccatcaaagc gttcccccac tatcgcaaaa aataaatttc ctggctttaa
    51481 tgtgcgggta tctatactaa caccagtaat gtcggcatta atctggcaag actgagaaag
    51541 aagtgacgca atagtaatta ggttcatagt attttgtgat gtcgaaaaga ctagtgtact
    51601 aacactcagg ctcacagttc aagatggttt gagaatagat agataagaat ataactaaaa
    51661 agtcaagaat tttaagttta ttataatccg attcataagt tatcggacat taaccattaa
    51721 cagagtggat aatgagcttc gcctggtgcg aagccctatc ctgaaaatat aatcgctaga
    51781 tagtgcaatg attctagcct attggcatta gtttgttaca caatcatcca tcaaatgctc
    51841 aattttcaag tatcgatgat atcgtgcgat acgctcctcc gttagaggtt tgcgctgttc
    51901 atccagcaaa taagcgtcga gcccctcact taattgtttt gcagtatcta acataacaga
    51961 aaaccgttct gcattaatct caggattatt tgatatttgc atgaacagac ataatcctct
    52021 tacactgaaa gcaccgatat tctgtaaatc aaaaacaccg gttgctgtag ctgcagctaa
    52081 actgcaaagc actggacctt gtccattcgt gaattgatgt ctatgaaaca agtgcctctc
    52141 accaaatctc aaaccagcag ccaaaacagt ttgcaataat tcataaccgg caaattgtcg
    52201 attttcctta gccaataaaa acaccatggc ggtggaagaa gaacaatcat tattaggttc
    52261 tgttctttca tttgattcgc actcttcttc ctcaggaatc aactgaggtt gttctgtttt
    52321 tacagagggc ttccttattg cttgtcgctt ttcagaacca tcaaaatttt caaaatccaa
    52381 aggaacctcc ctattgacct tccgcacagc gataatttca tcattatatg attgagagcc
    52441 ataggagtta ttttctactt ggccaagaga aggttgatag tgttcttgat ttaagctctg
    52501 tcttctagtc ttcattaaac ggccaatagc cactagaacg ccaattaaca acaggacatt
    52561 aagaattaag ctccaatttg cctgcataag tttactccat agtcaaagct tgtttcacat
    52621 caaccgcaac caaacgagac actcccggct cacgcatggt caccccaact aaatgttcag
    52681 ccaattccat agttacctta ttatgagtaa taaacaggaa ttgtatgaat tgtgacatct
    52741 cttttactaa atcgcaaaac cgacttacat ttaagtcatc taaaggcgca tctacttcat
    52801 ctaacataca aaatggtgaa ggatttagct gaaaaattgc aaacaccaaa gccacagcag
    52861 tcattgcctt ttcaccacca gataatagat gaatagaact attacgtttt ccaggcggct
    52921 gcgccattac tacaatccct gcttctaaca gattatcaca agttaattcg agctgtgccc
    52981 gaccaccgcc aaataatctt ggaaaaagcg ctttaaaaga ggtgttcact tcatcaaacg
    53041 tattttcaag acgcgatttc gtttcattat ccattttttc aattgcagtt tccaaagtag
    53101 ataaagcttg gctcaaatca ttatattgct catccaggaa cactttgcgc tgctgttcgg
    53161 cagcaaattc ttcgatggcg gccaggttta tagcgcctaa ccttttaatt ttctccaaaa
    53221 tcgctatcaa ttcgtcttct tttatacttt gcgtgactcc ggatggaatt cgctctaata
    53281 aagactgggc ttgtaaatta gcctcatcaa gagtttcttg aattgaactg gcacgaacgg
    53341 atagggcttg tttttccatt tggatttgat tcatgagttc ctgaatccgt tttacttcct
    53401 gatcatagtt taaaattaat ttctcttggt cttcaagttg caacttaagc tgagatactt
    53461 tttctcggct caaagtcaac atcgattcga tttcaccatg ttttatcaat aatgctgcca
    53521 attgattttt aaactcggct tctggcgcta ctgactgtaa ataatgttga gttaactcct
    53581 ctaaacgcat ctgtgcacgt tgcaaacgct ctttttctct acttatccta tcggttattt
    53641 gctgtatctt ggtgacttct ctatcatatt ccaactcact ttgatgcaac aaagcacgga
    53701 cactctctac ttgtttattt tgttcaacga gaacactgtt ccatgtgtct ttttcattta
    53761 taaatcgctt gaattgcaac tcagaagctt ggtattgacc ttccagttct ctgattttat
    53821 cagacatttc caattgttca gcgtttagct cttccagcga cgactccagc tctattatct
    53881 cgttcgtgat agcatctttc tgtttttcag catgaaaaat agcttgttct tgattattta
    53941 ccgcaatgat atgattgcgt agcgcctcat tactggcatt taaatttaat tgatgcagtt
    54001 tgataaaatt taaattttcc tgtagccgcg cttgataact atctcttgct aaacggatgg
    54061 attcaatttt ttgttgtaaa gattccacac ttaaattcaa ctcgacaatc ctttgctgtc
    54121 tggcaagaac gcctacctca tcttgctctt tttggcgagg taatttaatc caccccttgc
    54181 ctagccaaat accattgacg gttacaatag attcatgagc caataaatca ggaaaccacg
    54241 cctttgcctc ttcaagattg tctgcggcat aaatgtgttc aagatggttt actgttgagg
    54301 gaaccaagcc ttgaatttta tcagctaacc ttgggcgctc ctgcttttcc ccattgagag
    54361 ctctcatcgt cactacgctc tctttatgat cttcgcaaaa ctgccaccga gaccatagct
    54421 cagcaaaatc atccaataca taagcgatta gatcgtcccc taatacgatt tcacaagaag
    54481 attgccattg gggttcaacc tttaaaacat ccattaatct tggcttatct gaccattccg
    54541 caattccgtc cttattaggc cgattttcct tttttgctgc aatttgagca gcttttaatg
    54601 cagcaagctc agtgtttaaa cgatgaaatt catcttgcaa tgcatgcagt tgttgctcgg
    54661 tattttgtaa ttgattctgc aaatccgatt gagtctcatt cgcttgccta agctgttcct
    54721 catcaaactc ctgtgtttct ttcaattgga aatgcttttc ttgcaatttg attttatttt
    54781 tttgcaaatc gtcaagcgag atagctgatt gttccaaatt cagtttttcc agccttacca
    54841 ggacttgatg gcgcttctgc tctaaatgtt gtagtttggc ttgagtgagt tggtattcat
    54901 ttttaatggt attggaatta atttgcacct cttgccaaat tttctcccat tgcgattgtt
    54961 ccttttgttt ttcttgccat agagattcct gttctttgaa ttccattttt aaatgatgca
    55021 aatgctcctc taactgttga gccttaagct tgcaattgtg caattcttca ttatccagct
    55081 taagttgctc ttcaatgagt tgagaatcaa cctgcatttg ctgcctgtca ttttccaaac
    55141 gctttttttc tctttcttgt tgctgaatgg cttcttcaag acgagctatc tcagttcctg
    55201 attgatagag agaatcctga attgattggg attgatcaat ggcatcttgt aattgttcat
    55261 ttaaaactgc ccgctccttg taacaactag ttaataaact ttgttgctgc tcattgcgga
    55321 ttgctaactc ttgtaattct cgctgtttta ccgtttgttt ttctgagtat tcctgccatt
    55381 taagagcctg gatctcagct cggcaaattt tttcctcttc ctttaaaatg gtatagcgtt
    55441 ctgctgcttt ggcctgccgc tccaaacgtt gcaaatgttt gcccaattct tctctaatat
    55501 cagcgacgcg cgtcaggtta tcccttgttt gttcaatacg ttgtaaagtt tcccggcgac
    55561 gctctttgta ttttgaaact ccagccgcct cctccaaata gactcttaat tcttcgggtt
    55621 ttgcttctat taaacgagat acagtacctt ggccaataat tgagtaactt cgtgcccctg
    55681 ctcctgtgcc taaaaaaata tcagtaatat cttttcgcct gcatcgagct ccattcaaat
    55741 aataaaaaga ttctccatcc cgagtgacta ctctctttac agcaatttca ccataactgg
    55801 caaaagggcc ggtcagtctt cccaggctat tatcaaatac cagttctact gacgcctgcc
    55861 ctaccggttt gcgattggaa gaaccgttaa agataatatc cgtcatggac tctccacgaa
    55921 gatttttggc agagctttcg cccataaccc agcgaacggc atcaataata ttggacttgc
    55981 cgcatccatt cgggccaaca acagcgacca aacgactggg aaaatgaacc gtggtgggat
    56041 caacaaatga tttaaaacca gccaatttta attgcttcaa atgcatttta actgcgaatt
    56101 ataaaataat aatcggttag tatatcgtag gaagtgaatc ctgactattt cagaacctcc
    56161 atatcgcttt cttctgccat cgttaaggac aacatgctca attgacaaac aattttatcg
    56221 ctttataatg catacttctg ttaacaaatg taggtaaaca atgaaacagc aattctataa
    56281 attgccccaa attctaataa gcctttgtct cctattgatt ctttccaccc caatccaagc
    56341 agaaccgcca aatcttagtt taattagaaa agaaattaaa aactattacg actctggctt
    56401 gtattatcac gaattggaac gaacgattaa gttagctcaa gaatatatac accaacaata
    56461 tttaatcaat aaaaacaaca aacacccaaa aaaacttgcc atagtactgg atattgatga
    56521 aaccagcctg tccaactatg ataagatggt aaaacgggat tttaccggta gcaaagagca
    56581 aattcataaa gaaatacttg ctgccaattc accggcaatc aagcctatgc tgacacttta
    56641 caaaaacgcc ttaaaaaagg gtatcaaagt attttttgta accggaaggc aagaatccga
    56701 gcgcgacgca acccgcgcta atttaattaa agcaggatat actaaatggg caggacttta
    56761 tctacggcct aatgggtatt cttccccttc gattatccca tttaaatcaa aagcaaggga
    56821 aatgatagca aaaaaaggat acaccatcat tgccagcatc ggtgatcaat gcagtgatat
    56881 ccaagggggg tatactaaaa aaggatttaa acttcccaat ccattttact acttgcctta
    56941 gtcaaggcgt gatgggtaat taggtttttc gtaggtcctg ttgattttga atctaagaaa
    57001 cctcaggtac aacgacccga ggtttcttaa taactacaag cattaagctg ctttttcttt
    57061 catacgaatt gccatgactt ccatagccag gaatttgaca taatcatagg tcacgtaata
    57121 atctccttga tctccagcaa atttggacca agaattacgc aaggtgaaaa cgcctttgtt
    57181 aacatggcct tcttcatcca tcacttcaag atcatcatca tagccagtga tcactaattc
    57241 gtgcccggca tagatcattc cattcattgc gtccaataca atttcaggtg ttaacatcca
    57301 agtgtcattg taagcacggt tggtacctac agctccggca tccccaacaa acacatccaa
    57361 taacatgccg atagtcaagc ggttaccttt cgccagctcc tccttgatct gatagacgat
    57421 ttgattcata tccgctttag ctgaaaagga ttcctcatcc tttaacaaag cctcccagct
    57481 aattaaattc gataccggga cgctatgcgc caggaactcg gagtcagaca tgggtttacc
    57541 ttcattgttt tcgtcttcta aaggatattc tctaacaccg gcgcaaccat tcaatttctg
    57601 ataattctga ctgatgatac cgtattcaga aatctgttga agaacccagt aaccaaaaga
    57661 accattccac ccgctggctt tggctttgtc atgtatcgcc agataacttc ctaactccag
    57721 attacacaat tgactgatgt agtctcctgc cccaagggca gcatctatgg ctgcagttac
    57781 tgcgaaagtg acacaactgc catgatatcc ctgatccaat actggtgtta actgcattcc
    57841 caacttaact tttcttggta actcagaaga aaaactggct gtgtttatac tatttttagg
    57901 atattctgac agctgttcac gcaaataaga tttcgcttcg gtcgataatt catacttggg
    57961 cagcttaaat gttaagcttt gagcaacacc actgggattt ttaggcgtaa tgtgaacatc
    58021 aacctctcct tttaaagtca aaggacctgc aaaagccagt gatgaagaga aagacaatac
    58081 agctaaatta attaacttac tcataaaata tatccctata attaaccctc ttgcatcata
    58141 tcaagaggtg aattaaatgc aaacaaatca tgcgaataag aataaaataa caagctaatt
    58201 ttctattcgc acagtagaaa ccattttata ataaggtgac cattcgctag agcgctgtga
    58261 taatttttat caaacaaatt ctatctcaaa tactgaaata acatcctctc attgatgaat
    58321 caaaaataac aaatcagatt caatttaaga aaacaagcaa cttgacccga gttcatatat
    58381 aataagcctg attctaaaaa agaggatatt aatatgcgta ctctattgtc atgtttcctg
    58441 ataaccctat tgtcttttgg tttaattatc aatgaggcct ctgctaaacg atttggcggc
    58501 gggcgcagtt ttggggttca acgttctcaa agcagtcttt tctcatctaa taaaacatac
    58561 aataagtctt cattaacaca aaaagctaat aagagcagat ggggtggcgt attaggcggc
    58621 ctcctgattg gaggactatt ggccagtctc tttatgggca atggcttggc aaatgggtta
    58681 atcacctggc taatccttgg ttcagtcatt ttctttgtag tcagcttctt acgcaggaaa
    58741 atgaatccag ggtttcaagc aactcaatcc ggagcattcc gccaaaactc ttttgaacaa
    58801 ttcacgcaaa actacggaaa ccgaaatagt ggaggagctc attttgccca acaagaaacc
    58861 agctttgacg cggacaattt tcttcgtgat gccaaggtaa cttttatacg tctacaagcg
    58921 gcttacgacc aaaaaaatct gcaggatttg caaacattca cagttccgga agtatttgca
    58981 gaaattaaaa tgcaactgga cgaacgaggc gatgccccca accaaacgga agtcatttcc
    59041 ctcgatgcaa aattgcttga tgtgtccaaa caatccttat ccacgatagc cagcgtacaa
    59101 ttcactggaa caatcaaaga gaacggtgaa atatctaacc tggatgaaat atggcatttc
    59161 cgccagtttg ataacagaaa agagtgggtt gtagggggta ttcagcaaga agtatttcag
    59221 ccataaatac ttaaggtttc ctggagtagc aactgtcttt aatgacagtt gcactaaaaa
    59281 tcaaagaaaa taactgaaat cactcaacaa tatattgcac ctggatgatt tcatattcaa
    59341 ccataccgcc aggcgtttct accgtgaccg catcatcaag ctccttgcca attaaagctc
    59401 gggcaatagg agaactgtat gaaatcttgt tcagtttgat atcagcttca tcctctccaa
    59461 ctattttata ggttaattct gagcccgttg ctacatgaca aattgtaacg gttgaaccaa
    59521 atatcacttt accattgtta ggtaatttac tgatatcaat tatttgcgca tgggacaact
    59581 tcgcttcaag ttcttgaata cgcccttcat taaaactctg ttgttctcga gctgcgtgat
    59641 attccgcatt ttctttcaaa tcaccatgag ctcttgcagt tgcaattgct tctacaatgc
    59701 gcggacgatc cacgaacttt aagcgatgca attctgcttt tagtgcttct gcaccctcaa
    59761 ctgtcatagg atgtttactc atatttaccc ctaatgtaaa tcttgtaagc gagttacagt
    59821 ttccctatct tcatacttca tagccaaaca agcagcttcg gctcctgata aggtcgtagt
    59881 gtagctgacc ttatgctgta aggcgttgcg tctgatagca aaagaatctg ctatcgcttg
    59941 tttgccctca gtagtattta caataaagtc tatttcatta ttttttataa aatccagcac
    60001 atgtggtcgg ccttctgcta ctttaaagac tcttctacag tctacgccgg ctgcttgtaa
    60061 tgccaaagca gtaccgcgcg tagcgataat ttcaaaaccc agttctatta atctcttggc
    60121 aatctcgccg acacgcgttt tatctgcgtc acgtaccgat acgaaagctc gtcgccgttt
    60181 ggcaatgtga catccggcac ccaattgtgc tttagcataa gcttgtccaa aacgcttggc
    60241 aatacccatc acttcccccg ttgacttcat ttctgggcca agaatcgagt ccacaccaga
    60301 aaatttgata aagggaaaaa ccggcaactt aatcgaatag aaagaaggcg catgatgatt
    60361 ttggtttaat ccttgatctt tcaagctaat tcctactttg caaagagccg ctattttagc
    60421 taatggcagc ccggtagcct ttgaaacaaa aggcacggtt ctcgaagcac gcggattcac
    60481 ttccaaaaca taaatatcat ccgcttgaat agcgaactgc gcattaatca agcccaccac
    60541 gcctaatttc aaagccattt ttcggatctg ctctatcaaa tcctgctgaa caactacgcc
    60601 taagctaaaa ggaggtaaag tgcatgccga atcaccagaa tgtatccccg cttgctcgat
    60661 atgctccatg atggcaccaa tcaatacgtc tttgccatca caaacggcat caatatctac
    60721 ctctatggca tcgttcaaaa atttatcaag cagaacgggt gaatcattag aaacttcaac
    60781 tgcatgcgac aggtaatacc ttaaatcctc ttcttgataa acaatttcca tagctcgtcc
    60841 accgagaaca taagagggcc taacgactag cggataacca attttgttag ctaattcaat
    60901 ggcctctgct tcgcttcgga cagttccatt atcaggctga tgcagtttca attctttgac
    60961 catttgctga aatcgttctc tgtcttcagc catatcaata gcatctgatg aagtaccaat
    61021 tatttttacg ccattagcct ctaaatcacg agccaacttt aatggtgttt gtcccccata
    61081 atgcacaatg acaccctccg gttgctccat tgcgacgatg gacaacacat cctctaaagt
    61141 aacaggttca aaatatagtc gatcggaggt atcaaaatca gtggaaaccg tttctggatt
    61201 gcaattcacc ataatagttt ggtaacccgc actccttaat gacaaggccg catgcacgca
    61261 gcaataatca aattcaatgc cttggccaat tcgattaggt ccacctccaa gaatcataat
    61321 ctttttctta ttgctctcag gacgtgcttc acagcttcct tcataacaag aatacatgta
    61381 ggctgtatca ctagggaatt ctccggcaca agaatctatt cttttgtaga cgggcaagat
    61441 ctctaattca acacgtcttg cccgaacttc tgcttccgta caaccaagca atttggccaa
    61501 gtacgcatca gcaaaacccc ggtgtttcat caagtgtaaa gtggttttat ccagatcccg
    61561 aatcgttttt ccaatgattg agcgctctaa aagaaccaac tcttgaattt gtgccagaaa
    61621 ccatggatcg actcgacttt ccagatgaat ttcctcaagt gataaatttt gcctaaaggc
    61681 atcagcaata taccaaagtc tatccggagt gggttctcgt aaatgccctc ttaaacgcgc
    61741 catatcctct ttttgaaaca gtgaatgcaa tccacatcgt ccaatctcta aacctcttat
    61801 ggctttctgt aaagattcct gaaagttaga accaatcgcc atgacttccc cgactgattt
    61861 catttgtgtg gtaagagtgt ctggagtttg cggaaattta tcaaaattaa accgcggaat
    61921 tttggtaacg acataatcaa tgctgggctc aaaggacgca ggtgttttac ctccggtgat
    61981 ttcgtttttc aattcatcca aggtataacc cacagccaat ttagctgcga ccttagcaat
    62041 gggaaaaccg gttgcttttg acgccaaagc cgagcttcga gatacacgcg ggttcatttc
    62101 cacaaccagc atgcgcccgt cttcaggatt aatagcaaac tgaacgttgg aacctcccgt
    62161 atccacacca actgctctta gaactttaat cgccgcatcc cgcatccgtt ggtattcttt
    62221 atcagttaat gtttgtgccg gagcaacggt aatggaatct ccagtatgca cacccatagg
    62281 gtcaaaattt tctatagtac aaacaataat gcaattatca tttttatccc ggacgacttc
    62341 catttcatat tctttccaac ccagaaccga ttcatcaatc aaaagctcgc gagttggtga
    62401 taactccaat cctctaatac aaatttcttc aaattcttca cggttatagg caataccgcc
    62461 tccactacca cccatggtaa atgaaggtct gatgatggca ggaaagccta aacgggcttg
    62521 cacttgaatt gcctcttcca ggctatgagc aatcgctgac ctcggcatat ccaatccgat
    62581 tttaatcatc agctggcgaa atttttctct atcttccgcc ctgtctatgg cttcacgcgt
    62641 cgctcctatc atttcaacag agtacttggc taatacccct tctcttacca aatccagggc
    62701 gcagtttaag gctgtttgtc ctcccatcgt cggtaaaaga gcgtctggcc tctctatctc
    62761 gataatacga gccacttctt tccattgaac gggctctata taggttgcat cagcaagctc
    62821 aggatcagtc attatggttg ctggattaga gttaactaat atcactcgat atccttcttc
    62881 tttcaaagcg cgtactgctt gggtacctga gtaatcaaac tcgcaagcct gcccaatcac
    62941 aatagggcct gctccaagaa ttagaataga ttttatatcg gttcgttttg gcataatgac
    63001 aataaaaaaa gaataaagtg atatatttta ccgagttttg cctaaagatt atacccttcg
    63061 atgacatgtt ttgagtcatt tagttgccca gaaaacagcc aaaggcatta aatgacataa
    63121 aattacttgt ataaattaga aagcagaatt tgcaaacccg cttctgtcaa agacagatat
    63181 ttataatagg gatcagctgt tgttcgctca agccattcat ttgcgatgca aatatccgtt
    63241 aatttctgcg cttcttccac tgaaacaggt ttgtccaatt tctgggtaag cattgctgcc
    63301 gcatcctctt caatactcaa aaaaacctgc tctctggtta ccccgcgcgc gatcatttca
    63361 tcataaaaca aggacagaat atccatttcg ttcatagtaa ttccttatta tttgatacta
    63421 agatctatag catatattta atcatcgttc gacatgataa aattaaatac tttttaatta
    63481 aaaaatttgt cataaccaaa tctctctatg caagaaatcg atgtacttat cattggagct
    63541 ggtgccgcag gtctcatgtg tgctattgag gcaagtaaac gcaaaagaaa agtgttcgtg
    63601 ctggatcatg ccaataaagc cgggaaaaag atactcatgt caggaggcgg tcgttgtaac
    63661 tttacgaatt actacattga acccaacaaa tatttttccc acaaccccca ttttttcaaa
    63721 tcagccttat cgcgttacac tcaatgggat tttattgagc tggttaacaa acataaaatt
    63781 gccttccatg aaaaaacatt aggacagtta ttttgcgata ataaatccaa agatatcgtt
    63841 gatatgttgc ttaaagaatg tgagcaatac ggggcgacaa tttatttaaa tactgtgata
    63901 gaacaaatac aaaaaaccaa cgactattcc ttcaagatta gcacgacaaa ggggaggttt
    63961 cattgccatt ctgtagtcat tgcaactgga ggactttcta ttcccacaat gggcgcgagc
    64021 ccctttgcct ataaaattgc agagcagttc aacattaagg tatggccaac cagggctggc
    64081 cttgtaccgt ttacgttgga tgtcctcgaa aaggatagat tatccgtcct gtcgggaata
    64141 ggcgttgata gcctggtcaa taatgaaaga aatcaatttc gcgaacacat cttatttacc
    64201 caccgtggct tgagtggccc tgctatcttg caactctcat cctactggta tcctggtgaa
    64261 aagatatgca ttaatttgct gcccgaacat aacctcctgg aaagcttgaa aacagcccga
    64321 gcggaagctc cgcataaaca gttaaactct gtattatcga tgtacttacc caaacgagtt
    64381 gttgaagtct ttattcctca aaaactgggt gaaaaaaaac tggctgacag ctctaataaa
    64441 gatttggaaa caatttccca tttgttgcag aactgggtag taaaacccaa tgggacagaa
    64501 ggctaccgta cagcagaagt gacaataggc ggagtagact gtcatgctat ttcatccaaa
    64561 accatggaag ccaacaatgt accaggccta tattttattg gagaagccct ggacgtgacg
    64621 gggtggttag gtgggtataa tttccagtgg gcatggtctt ccggttgggc tgccgggcaa
    64681 gtagtgtaaa taacggattc aaaaaaatca attctcaagg cgcattagct taccggaatc
    64741 ccaaagccca aattttcccc aagatttcat gaagagcaat ggaagtatag gccaggttat
    64801 aagaattagg tatcaccatt ttgacccagg tatcagtaga ccaaaagaag gtaaaatcag
    64861 ttggggctgc tatgggatgc aatccttgct gctggcataa tgccatggaa cgtggcatgt
    64921 gaatcgctga ggtaaccaga taaaagggtt gatcatgtac tatggctgct aattcacgcg
    64981 cctgatctgc tgtattgata gaagcagttt caatgacaac tttttccgaa tcgattgcaa
    65041 accagctggt taattctgtc aataataaag cctctgattg ctcaccagca gatcctccgc
    65101 ccgataaaag caattttgca ttgggcaaag ttctgaataa tcggacacct tcaatcaatc
    65161 gtttgatgct cgctgaggtc aacagatcat ttgcaggcat tccttcccca atattttgtc
    65221 ctccaccgag aacgaccacc catttcacat ttggattaac ccgcttcact atagggtatt
    65281 gagactctaa ttgataagtt aaataactgg gtaaccaacc cgtactcata gcaagcaaga
    65341 caatcaatac aacaaacaac ataactttag tgaaagtagg agcaagccga cgatatagta
    65401 aaaaaatgca aatacctaat accaaaagac ataaaaagaa aggatgcagc aagtattcaa
    65461 gcaaatgtct aatgaatgcc attatctgac tcttcgcatt aaataaattc caattaaaga
    65521 aaagatgaga gtgggactaa aggcagccaa ttcaggcggc cattggaata ctgtgctcac
    65581 tgggccaaaa aatcgattca gaatatgaaa cccgaatcca accatagcgc ctaccagtaa
    65641 ttttgaaccc atggttgacg aacgtaaggg gccaaaaata aaggggatag ctaatatcat
    65701 cataaccata gtattcaaag gctgggtaat tcgttgaaaa aaggcaaact tataatttga
    65761 tacattttga tggttgcgtt gttgctcacg taaatagcgg ttcaattcat gtaaagtcat
    65821 ttcatccgga ttggtgcccg taattttaag aatttccggt ttcactggca catcccacgg
    65881 caaggtatcg aaatgatagg ctttggtatg atcctctgca aattcagttt gtttcacatc
    65941 ataagctatc cacgaattat tgacatactt tacttcactt atctggcgag ccagttttaa
    66001 attatgctgg ttgtcaaagc ggaattgatt tacatagcgc agagtattct cgtgtgtgac
    66061 tgaagcaata gaaacaaaat cattaccatt acggacccaa acccctcttg gagtcagcaa
    66121 agcctgccct cctgttaaag cggaaacctt gtattcattc gcataatgcg ccattactgg
    66181 tacaatggtc tcacctaaag acgtgattaa tagaatgact atcagagacg cctttaatac
    66241 cgcccctgtt atttggccga tggatatccc tgccgcacgc atcacaacca gctcactatg
    66301 attagctaat acccccaaac caaccaaaca cccgagcaag ctggcaatag ggaaaaacag
    66361 atagacttgg tagggcatct gtaaaaggac aaaaacgctg gcttgtacta ttccatagtc
    66421 tgctcgcccc aaatcgtcta tctggttaac aaacaaaata aaggtctgca aaccgaccag
    66481 catcaaagtt accaagccga ttgcagataa aaccgttttg gcaatataac gatcaagtat
    66541 ttttctgctc atgcaagctt cacctgattt ctccaaataa ataggatgcc gagcatggca
    66601 acggcgacgt gtacccacca catccctatc caccagggaa tcttcccggt agcaatcgca
    66661 tcacgagaaa caaacataaa attagcataa aaaatataga taatgatggc tggcaaaagt
    66721 ttcgaatatt ttccagaacg aggactgaca cgactaagtg gaacagcaac cagagtcagg
    66781 gtcaaaacca ttaaaggaat ggaaatacgc cactgtaatt cagccgcctt tttcaaatca
    66841 tgattgttca aaggccataa gtctctggtt tttgcagtgc gggcatcatc attcataaca
    66901 acagtcggat tgggcaattt ggatttatag cttgcaaact ctgcaatctg gtaatcggca
    66961 tgaccaggga caccttgata ttccttgcca ttttgcagca cgatgtattc ctcgcctgtt
    67021 tgaggattaa cttcagcaaa tgctttatct gcccacaaca catcccatag cagcctatta
    67081 tcctgaacgc tatgttttgc catgaatatc tgttctgcct gagagtgatc acgattcatg
    67141 gattggacat aaaaaatttg ctgaccgcta ttaatggcgt gaaagcgccc aggcataatc
    67201 gtttgaacta aagttttgat tcctgtagaa cggattaact tggtgcgctc aacagcaatc
    67261 aaaggacttg cccaaatcat tacgatggca acaactacta acacaacagt agccatggca
    67321 aagctgtgtt tcagtaattg cttggggcca taaccacaag ctcgcagaac cgtcatttcg
    67381 ctttcggcat ataaccggcc atacgccaat aaaagagcaa cataaaatcc caggggcagt
    67441 aacaggccca ttaaatttgg caactccaac atcaacaact tcataatcac aaaaccagga
    67501 attgaacctg aagcagcgcg atttaagtat tgtatgaatt gattactcat gaaaataagg
    67561 actaatatcg cggttaatgc gattaaagtg agaaacactt ctttagtcaa ataacgaaaa
    67621 ataatcacta caatacccta cagaggacct tatcaattaa ttcaattctt tcagaataac
    67681 atatgaaaga acccatcatg ataattcaag acagcctatt taaaaatagt attgatttaa
    67741 aacagatgat taaaacagaa tttttcattc aaatgctcat ccaatttagg taaactagtt
    67801 gtctttctca agcggtgcag gaaaaaatat tatgaattat ggattaactc ataccccctc
    67861 attaaccacg agcgaatgtc tggtgttagg cgttttttca gatttggcgc taccagattt
    67921 tgcaactgca attgataatg aacagcaagg gctgattaaa aaactttgcc agagagtccc
    67981 tgaaccagga aatactgtat ggcaaactga tgttgaagga catagcctat taataattca
    68041 gtgtggcaaa aaggaagagt ttagtgctaa ttcactccaa aaacgtgtag gagaaattac
    68101 ggaagcatta attaaacagc gtttctcttc tgccaccgtg tgcttgcctc ggttaaatca
    68161 ggaatctgct gaatggcagt tagagcaaat gattgttcaa atcgataatt tacgatatca
    68221 acttcttgat tttaaaacga aacatgccaa aagtcataaa ttggagtcgg taatattcca
    68281 tcttcctgga gctacagaaa aaagccttga aatggccaaa gccattgtta ccggagttga
    68341 attttgccgt gatttagcga atatgccggc caatatttgt actccgactt accttggtga
    68401 gcaagccatt tcattatcca aacaattcga ccaaataagc tgtcaagtga tggggccaga
    68461 agaaataaaa gaaatgggta tgggcgcatt attagccgtt gcccagggaa gtgaccaacc
    68521 cccaagattg atagacattc attatcacgg taacaaaaac tcagcaccgg tcattcttgt
    68581 cgggaaaggc attacttttg actcaggagg cttatctata aagccggcca acgccatgga
    68641 tgaaatgaaa tatgatatgt ctggcgctgc cagtgtgctg ggagttataa aagcctgtgc
    68701 tttgctgaaa ttgccaatta atcttatcgg cattatagcc agtgctgaaa atttgataag
    68761 tggctccgct gtaaaatcag gagacattgt aaccaccatg tcaggacaaa cagtcgaaat
    68821 tatcaatacc gatgcggagg gtcgattagt gctagcagac gctttgactt acgcggagcg
    68881 atataatcct gattttgtaa tcgatattgc tactttaaca ggagcgatta tcgtcgcttt
    68941 gggcaatata gccactggct atatgactag agatgagcag ctggccaaat caatcgaatg
    69001 tgccgctaat gaaagccagg ataaagtctg gcgtatgcca ctggatgagg cctatcaaga
    69061 tgctttggaa agcccattgg ctgatatgat caacgccggc tttgatcgca gtgcaggaag
    69121 tattactgcc gcctgtttct tatcacggtt tactgaaaaa tatcgttggg cccatttgga
    69181 catcgcggga acagcctgga tttctggaaa gaagcgtaat gcaacagggc gccctgttcc
    69241 tttattaatt caattattgc gccatgtcgc caattcgcgt tgatttttac ttattagcca
    69301 gcagccttcc agaatctcgc tggttggttg cttgtcgttt aatagaaaaa gcctatacca
    69361 aaggacatac tatttttgtt ttttgcgaca atcagcaaca agcagaatac cttgatgaat
    69421 tattgtggac tttcaaagac gacagtttta ttcctcataa tttgcaaggt gaaggaccgg
    69481 agcctcctcc tcccgtacaa attggctatc aaaaagaacc cagaggcttt acagatatcc
    69541 tgttgaactt atccaccaat attccaattt tttatacccg atttaaaaga gtcattgaat
    69601 tggtgagtaa tgtagaacat gagaaagaag tgagtcgatt gcactataaa gaatatagaa
    69661 acaaaggctg tgcattgcac acacactcaa ttgaataaat atttttctga tcgcttgatt
    69721 tttaagctta ttgtgtatcc gctccctgat ggtcgcgtct ctgattataa taaagtaaaa
    69781 cagagccgcg accatcaggg agcggataaa ggataaagaa gcccggagaa tcagcagtta
    69841 cattttaacc tggtgatttt gaatgagacc acagcacatc acaacccttt ttttccgcgg
    69901 cttgcaataa gtgaattaaa gggattgctc gtttagccaa actgatatca ggctcactat
    69961 cctcatcgtc ttctagcggt ttattttcgc tttttatttt tcccagccct tgttgtaaat
    70021 gatgcaaagc atctagtaca ttttcagact taatagcacc gggaattgtg cccccatgcc
    70081 ccattaacaa caataattgt tttgccacat tttcaaaata agtaatgttc tcatatgcat
    70141 cacaactaaa agtcacaaac ataactaact cccaactgaa aattctatcc gtaagtctgt
    70201 aataaatact cggcaaccgc tcgcaagccc attgcttcac caccttcggg ctttcctggc
    70261 ttacttacag tattccaggc catgatatca aaatgaaccc atggtattga ttttgccaca
    70321 aatcgttgta gaaataagcc cgcagtaata gcaccagcat aggacgaaga actggaattg
    70381 ctcatgtctg caatattcga attcaataac tcttcgtatg gtgaaaataa aggcaaccgc
    70441 cagacagggt ctgagacttt attagatgcc gacactatat cctgagccag ttgatcatga
    70501 ttagaaaaca tggctgcaat ttcagttccg acagcgacac gagcagcacc tgttaaagta
    70561 gcaaaatcaa tgatcaaatc tggtttttct tcacaagcct taaccaaagc atctgccaga
    70621 atcaatcgac cctctgcatc agtgttttct atttctacgg ttaatccatt gcgcatggtt
    70681 aaaacatctc ctggcctaaa agcattcgga ccaatggcgt tttcaacagc ggggattaat
    70741 acctgtaatc gaatcggcaa acgctgaacc attatccact gggctaaacc tatgacgtgt
    70801 gcggcaccgc ccatatcttt tttcatcaaa cgcatccctg atgaaggttt gatatcaagc
    70861 ccaccactat caaaacaaac accctttcca atcaaagtta cgcgtggatg tttctcattt
    70921 ccccaatgca aagataataa gcgaggcgct aaagcagaag ctcttccaac cgcatgaatg
    70981 gcaggaaaat tatcctttat caattcctct ccaatccatt gcttaaagta agccttgtgt
    71041 gttttagcca attcttcgac aacatgagct aacgattctg gccccatatc gcaggttggc
    71101 gtgttaatga gatcccgcac gagaaataaa gaattcgcca aggataaaat ataatccata
    71161 tcatttgcct caacaattaa aactcgaggc tgtaactcgt attttttata ctcatcaaat
    71221 cgatattgtg ctaatgccca aactacgcca gactcacgac ttaacttatc ttgaaattga
    71281 tagttccctg gaggcaataa aagtgcggca ttggccaaag cactttcctg attaccagtt
    71341 cccgatccaa taaaagcttt ttcaatcacg ccatccgcat tgaggataaa acaataatcg
    71401 cctaatttgc ccttaaactg gcgtacagaa aaacaatttc gttccatggg ggttagattc
    71461 tcaattcctt cgtcccattg cgattgagaa atgagatgga caggaactac cctatcgccg
    71521 caacttttgt aaaatacttc tgcttgcatc tttttaatcc tttatatggc cacgaaagtg
    71581 gctaggtcta agccctatta tacctaaaac taataaatag atgactacgg tgacgaacac
    71641 atgagctaat aacaagctca aacgcatcaa gggagagaaa ccaagccagt aagtcgctgt
    71701 tccactcata aaatataaat aaaccccaat ggcaaaattg gctattaaca actgcatact
    71761 gtatttcagc catccgggag atggtttgaa caatcccctt ttaatcaaga gaaacagcaa
    71821 aataacacaa tttacataac cagctaatgc tgaagccaac gtcaagccag catgagccaa
    71881 atgccagaca aatacgaaac ataaaagagt attaactacc atggctatgg cacccacttt
    71941 tacaggggta ctgatgtcct ggcgagcata aaaaccagaa gccaacactt ttactaccat
    72001 aaaggcagga actcccatag caagagtaat caagcttttt tgagtttgta ataaatcata
    72061 ggcagtaaat ttaccataag caaaacaact ggctatcaga ggcaaggcaa aaaaggacaa
    72121 tcccaaaccg gctggaattc ctattaataa aatcgaacgc agcccccaat ccaaagcgcg
    72181 agaatattga ttaatatttt gttctgtgtg ttttcgggac aggtggggca agataactgt
    72241 cgcaatcgcg accccaaaga cccccaaagg aaaatcagtt aatctgtctg tatagtataa
    72301 ccaagagaca ctccctacct ttagaaaaga agcaaaaata ctatctatca tcaagttaag
    72361 ttgcgcaatt gaaacgccaa ataaagccgg gatcattaac ttgagtactt tattgactcc
    72421 tggatcatcc cgaacaactc tcggcctgat aagcaaatgt cgttgataca aaaatggtaa
    72481 ttgaaaaacc aattgcacga taccggcaat caacacaccc catgccaaac caacaacggg
    72541 ttgtggcaaa ttagggcaca aatacaccgc agcaagtatc atactgatat taagcaaaac
    72601 gggagtaaag gctggaaccc cgaaataacc ataggtattt aatattgccc ctgccatcgc
    72661 tgttaaggaa ataagcatta aatagggaaa agtgatacga agcatctgag ttgccaattc
    72721 agcccggacg ctgtcatgat ggaaacctgg cgcgaataaa aaaataataa caggcgatgc
    72781 tacaattccc actacagtga ccagagttaa tatagaactc aaatagcctg aaatccgagc
    72841 gatgaaagta cgaacgtctt cagcagaacg cgttttttga tattcagcca ataccgggac
    72901 aaaagcttga gaaaaagcgc cttccgcaaa aaggcgccgc ataaaatttg gtatcctgaa
    72961 agcaacaaaa aaagcatcca tgccagcttg agccccaaaa aaattggcaa gaaccatatc
    73021 tcgtgcaaac cctactatcc ttgaaataaa tgtcattaat gaaacaaggg aagtggatcg
    73081 tatcaagctt tgttttttag gtaccataat atccgttgtc agcatgatgt tttaaataga
    73141 taaaaatttt ctttatgata tacttaaact ggcgcgagtt cgatataaaa agcatggaaa
    73201 tacctcttat atctgtttaa gctggcatta tccagtaaaa cacaacacat caggatacgg
    73261 gttccactgc aaatgaagtc gagcatggta tcaaatgaat aagtgaatca cctgaaatat
    73321 tggttttaca ggatttatcc ggataataaa acatttcaac atcaacctgc gttaaaacaa
    73381 atacataaat acaaaaggtt caaagcaggt ctattgacaa attctaactc attgtgcaag
    73441 atctacatct tttgcaacat ctgattgagt ggagattttt aagtggcaaa tatcaaatca
    73501 gcgattaagc gtgcacgtca aaacgtcaaa ttgcgtcaac ataacgccag cgcgcgttcc
    73561 atgtaccgca cctacattaa aaacgtgctg aaagccgtag aatcaggaga tcaagaagct
    73621 gcgcgtgctg cttataccaa agcacagcca gtgatagaca aagccgctaa taaaggctta
    73681 attcataaaa acaaagcagc acgtattaaa ggtcgtctgg ttgcgcgtct taaagctatg
    73741 gctgcctaat cgagagtttc cgtcgctcta agttcgaaac taccgaaccg gaattcccgg
    73801 ttcggttccc tagtgtagcc cgttagaata attgcttgcc tcgatgagag gtttttcatc
    73861 atctgttatc ctgggttctt ggctttgatt ccctttgata aaagttgaat aaaaataatc
    73921 tctgcccatt gctgctgcaa ccccctggaa aaagcctaac atacttaatg gttgagcagg
    73981 aaacagtaat gcggctaatt ttctttgtga ctgcatgtgt atcactatat ccaattccga
    74041 ggacatggca tacacatttt ccttggctat tttttgctca ttctctactt tttctatttt
    74101 gccctgtaac tctttttcca aagaacaaaa aggcgccatc atcaatttgg caaattgttc
    74161 tgtaattgct tttaaatgtg agcgattcac ttcaatgctg tcgattgtcg ggttagtaaa
    74221 gccatttgta gtaaagagcc atgtggccac ttcattaagc atgctatgcc aaggtaaaga
    74281 gggcagtaaa aattttgtta gccaatacct gtcgtctttt acagaggaaa aagcagcagc
    74341 caggcactca acaaagaaat ttaatgattc attaaagtac attatccgtg catccaattg
    74401 ctccttataa atccctaaac actcatgaca aaaatccgat gccaatttat attgtttttc
    74461 tgctatctgc agacgttccg cataatactc tacagtttca cgaccctttt cgacatcgcg
    74521 ccactcctta ttgatactgc gtgccatgcg ataagccaaa tttaagcctc taaaatgaac
    74581 attagtttgg acttcaaagg ttaattcctc caatgtatgc aattgttcca ttaaggtagt
    74641 aagttggcat aatctcttga ttacttgatt attttctcct gagtggcata cataaatcac
    74701 tctgggcact gttcttgagt tgaattgctc ggggaaattt tctgagtgga tcaaagacga
    74761 ggattgctcc agactatgct gcggaagttc accctccgat tgattgacca atattgcatt
    74821 gcggatcagg ggaaaacctg tggcttttaa ttgctcctgc attgcttctt ttgtcgaggc
    74881 ttgaaatgca ttgagccaat ttgccttatc ttttgaattg attgcttcct tttgagtatc
    74941 cagccaattt ttggctaaaa catacagtct ctttttagcc aattgaattt ggctttgtaa
    75001 atcatttatg gttaaaaaca tccttttatg ctcacttgtt tgcttgagtg accttaatct
    75061 atcactctaa aatgcatact tcaatcaaaa aaattcattc ataaccggat actttaatac
    75121 aaattgttta ttaa
//
LOCUS       DASLOE010000002        67663 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.002, whole genome shotgun sequence.
ACCESSION   DASLOE010000002 DASLOE010000000
VERSION     DASLOE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 67663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67663
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.002"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            381..527
                     /locus_tag="U6309_000066"
     CDS             381..527
                     /locus_tag="U6309_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503538.1"
                     /translation="MKQSHWMVLACCLVLTACMSSGTSSKYTKSLEEHVAQGHVGHGG
                     GGGR"
     gene            679..3366
                     /locus_tag="U6309_000067"
     CDS             679..3366
                     /locus_tag="U6309_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN5503539.1"
                     /translation="MAEQWHEKAPADMVLLFQTDLSLGLSEKVAEQRLKEVGPNLLSQ
                     QKKTSPFVIFLQQFASVVIWVLLGAVVVSFLLDEKADAIAILAIVILNAIIGFFLEYR
                     ADRAMLALQQMAAPKATVLRDGHAKVIAASDIVPGDILLFESGDLIAADARLFELSAL
                     KVNEAPLTGESIPVAKNLDVCTAETPLADRKNMVFMGTSIADGTGRALVVATGMQTEM
                     GHIAKMLGEASRDETPLQKKLNQVGSRLLWLCFFIIIAIFGLGLLRNISLFSLFMSSV
                     SLAVAAIPEGLPAVVTVALALGVQRMVRRAVLVRRLSAVETLGCLQVICTDKTGTLTV
                     GEMTARQLVTASDVYSIHGEGYNLSGGFTLQGQEINVSEDPLLQATLHAMVACNNASF
                     ESQDGQVATVGDPTEVALLVAAAKGGVWQGELQASYPRIKELPFSSERKRMTVVCRQD
                     HEHIAFVKGAPEIILERCTHILTKTGIKKVTPNDKARMKQSCELMASEALRLLAFAKR
                     QLEPTTLEEEEEIEHNLVFLGLIGLQDPPHASSKESISRCKKAGIKPVMITGDHPDTA
                     RAIAKELGILEAGDRLLTGNELENMSEEEFNHCVKDIAVYARVTAEHKLKIVRAWKKQ
                     QMVVAMTGDGVNDAPALREASVGIAMGKTGTAVTKEASDIIVMDNNFTSIVAGIEEGR
                     TIYDNIAKTLAYLLAGNSGELLVVFVALLIGWPLPLLPIQLLWINLVTDGLPAIGLAT
                     DMSEPGILNRPPRATQKSMMDMAFFKRVTFVGCLKALVILSVFAYEYLIDKDLIQAQD
                     AAFSVLVTAELLWAFGARSDTKNIWQVGLFSNLRLFFIVSISFSLQVLIHHIPVLREL
                     FGIQPVSFTQCLVWILLGMVPLLVIEAQKRLRKAPNEAF"
     gene            3353..4612
                     /locus_tag="U6309_000068"
     CDS             3353..4612
                     /locus_tag="U6309_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEN5503540.1"
                     /translation="MKHFSAKKMIWTILLGFCCFAGPVSYAKTPPALSQLINEATQNN
                     PQIRAARDRLLAAIHVIPQAKALPDPKLNAGYINMSENIPMDVDPRREQMLGGQQEIP
                     FPGKLIVRGRIATLEAKRAEAEYQATSFAVIAELKRLYYDLYFVNKSIEIVQRNQELL
                     HEMEKSSEANYSVGKTPQQDIYRAQTEISRLLMRLVILKQQRGSLQADINRLLNRSLE
                     ITIHTPAVLPVTPMGHNLEYFYTLVKSRAPQLIMQQRAVQKGRQAIRLSKMEYFPDVE
                     IEGGRLHDTGMHTKGYQVLLKATVPLYFMQKQNHAVRESLARYNADIEDLQTTYRMLS
                     FQVKNAYLLAERSAKLIHLIQHTIIPQATLTFTSSQATYGVGKVDFLTMLNNLLTLQE
                     NELEWHGELAEHEKAIAQIEESTGTFL"
     gene            4609..5742
                     /locus_tag="U6309_000069"
     CDS             4609..5742
                     /locus_tag="U6309_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEN5503541.1"
                     /translation="MNFKLIIKSAVILSLLSLLQVTQAQQAMDNHGKMDMKQSTQGNE
                     IVIDPARLQAIGITSEPVRRQVVEKIIRTVGRVEADERRVAHIHVRFDGWIENLFINF
                     TGEKVKADQALFTVYSPELVATQQEYLLALNAQKILSKNTTSSAARGAQSAFQAAHQR
                     LLLWGISEKDIEQLRRTGKITRTMTIHSPIQGTVLKKMALVGMRIEPGDELYTIADLS
                     RLWVLGDIYEYELPYIRLGQMADLTLTYLPNELFKAKLDFIYPTIDMKTRTAKVRFEV
                     DNQKEQLKPGMYVNLELKVPLGERLVVPKNAVLLTGERAVVFIYHGNGKIEWRDVTLG
                     VRAGDLLEIIKGVKEGDQIITSANFLIDSESQLKAAMGGMQHQ"
     gene            5821..8964
                     /locus_tag="U6309_000070"
     CDS             5821..8964
                     /locus_tag="U6309_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEN5503542.1"
                     /translation="MVERIIEFCARNRFIVLLFVLGFSFMGYMALKNTRMDALPDISD
                     TQVIVYTTWMGRSPDLIEDQVTYPIVTALLSAPKVVAVRGFSDFGFSYVYIIFEDGTD
                     IYWARSRVLEYMSQLSGKLPEGVTPQLGPDATPVGWIYQYGLVDDNGKHNLAELRTFQ
                     DWYLRYWLRAVPGVSEVASVGGFVKQYQVNLDPVKVLAYNLSVPEIVEKIRMSNNDTG
                     GRVIEFSGVEYMIRGRGYIKSTEDIEKIAVGTNANGTPILLRDVATVQLGSDMRRGVV
                     DLNGQGEAVGGIVIMRFGEDVLQVIGRIKDKLKELASAIPEGIKMVPVYDRSHLIREA
                     ISTANWNLIEELVVVGLLIIVFLGHFRSALIPIITLPLAILISFIPIYFLHVGLNIMS
                     IGGIIVAIGDMVDAAIIMVDNAHKRLADWEAKGSPGDRTQVLIDSAKEVGPAIFSSLL
                     VIAISFMPVFTLEAQEGRLFSPLAYTKNLAIFMSALLAITLIPVLLPLLVRGRIIPEQ
                     KHPITRLMQTMYAPVLKLALRYRKFVMGIAIVLAMATIPLYKLIGSEFMPPLYEGTIL
                     YMPVTLPGISVTQATALLQEMDKKLKAFPEVAHVFGKTGRAETSTDPSPFNMMEVIVE
                     LKPKEFWRKGVTYESLVKEMDEALQFPGVSNAWTMPIKARNDMLTTGIRTAVGIKIFG
                     PDIKKIEAIGKEIEMVAKEVKGTSTVYAERVAGGYFLDFQINRDQLARYGLTIMDVNR
                     IIESAVGGENIATTIEGRERYPVNVRYLRELRDDPDKLNRILVKTPSGAEVPIAQLVT
                     LTFRSGPAMVRDENGMLAGYVFIDISGRDIGGYVEELKQAVKVKVKLPPGYTLAWSGQ
                     YEFMQRVYERLKIFVPLTLAIIFVLFYFTFRTITETLMVMLGVPFALFGGFLLLYVLG
                     YNMSIAVWVGMIALAGVAAETSAVMLAYLDSDYHEQKEKGGLRTLSDLIQMVQQCAVS
                     RIRPMAMAGLANIIGLLPVMWATGIGADVMKRLAAPMVGGVFSALLLTLIVIPVVYVM
                     WRGQMDLKKTADLTEAR"
     gene            8961..10322
                     /locus_tag="U6309_000071"
     CDS             8961..10322
                     /locus_tag="U6309_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN5503543.1"
                     /translation="MKLTFLGATETVTGSKYLLTVGSKKILIDCGLFQGYKELRLRNW
                     APLPIDPHDIDAVIITHAHIDHTGYLPLLVKNGFQGKIYATPGTKALCSILLPDSGHL
                     QEEEARLANKYGFSKHQPALPLYTEKEAHTALQYFETIDFDTPHQLFHDFSFVYHRAG
                     HIVGAAMVKIKTQKGSIVFTGDIGRPYDPVMKAPAFIQETDYLVMESTYGDRLHDATD
                     PLPQMAQVINQTVKRGGSVVIPAFAVGRAQSLLYFIYELKRQGQITKDLPVFLDSPMA
                     IDATHLLCTYKEDHHLTAEQCRGLCHVATYVNTPEESKEIDRHHMPQIIIAASGMAQG
                     GRILHHLKAFAPNPKNTLLFTGFQAGGTRGARIVNGEKQVKIHGSFIPIQAKVVVMSS
                     TSAHADYPELLEWLTHLTNPPKKVFITHGEPHSAQSLKQKIEKQLGFSCTIPSYLQTE
                     ELW"
     gene            10319..11833
                     /locus_tag="U6309_000072"
     CDS             10319..11833
                     /locus_tag="U6309_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HEN5503544.1"
                     /translation="MSKQTSHGLRLKHLGIKTYHEAIIYMREDCHVCHSEGFEVQTRI
                     QVTLGQRSIIATLNVVTSELLQPGEAGLSDYAWESLHAKEGDEIQVSHPKPLESLSYV
                     HTKIYGKELSYEQMKVIIDDVLSGRLSDVQISAFLAASASGRLTSTEVMKLTKAMIDS
                     GDRLSWPSPLVVDKHCVGGLPGNRTTLIVVPIVAAFGLMIPKTSSRAITSPAGTADTM
                     ETLAPVHLSPQKMRQVVEQENGCIVWGGAVSLSPADDVLIRVERAIDLDSEGQLVASI
                     LSKKIATGATHAVIDIPVGPTAKVRNQSMALLLKQLLEEVGNELGLVVHTLLTDGSQP
                     VGYGIGPSLEARDVLAVLQGLPDAPNDLRERALTLAGAALECSSKVQPGLGKSIAKQL
                     LESGQAFKKFQAICEAQGGMRELTKARFTHPVVAAKAGKVSLIDNRKLAKIAKLAGAP
                     KSKSAGIDLHVHVGESVEQGEPLFTIHSESSGELHYACDVLRDKQDVILLGESP"
     gene            11830..12762
                     /locus_tag="U6309_000073"
     CDS             11830..12762
                     /locus_tag="U6309_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEN5503545.1"
                     /translation="MKPLLFSLFDSSEIARRLQEVLQVDVGDVTFHRFPDTEWYLKIN
                     SEVNNRSLMVVDSLNEPDEKILPLLFFAKTAKELGARKVGLIAPYLSYLRQDKRFHQG
                     EGITSRYFAQMLSSSFDWLMSIDPHLHRYHSLDEIYSIPTFVLHATSMIATWIKHHVP
                     KPILIGPDMESEQWVADIAEKGRFPYVILEKIRRGDKEVTISVPTIPELESSTLVLVD
                     DIISTARTMVETVKQLQQAGGKSIVCIGVHALFAEDAYSLLSEMKGVQVITCNTIHHV
                     TNAIDVSDLAVEVLVKNKLAALKSKLNKLIINKE"
     gene            12766..12933
                     /locus_tag="U6309_000074"
     CDS             12766..12933
                     /locus_tag="U6309_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503546.1"
                     /translation="MFKAFRMIAVLIGLIGGLFFLSSCDGAVKHGSQEVDGGYGGHSA
                     GGHGGHGGAGH"
     gene            12997..15213
                     /locus_tag="U6309_000075"
     CDS             12997..15213
                     /locus_tag="U6309_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN5503547.1"
                     /translation="MKHDHHQGHTHTDKEPACFHKEHGLHKRQDTASKTDGAVIYTCP
                     MHPEIRQSNPGICPICGMTLEPESITTEEGASPEYLDMRWRFWVGLVLTFPIVLLEMG
                     AHGFGHFISANISNWIQLLLATPVVLWGGWPFFVRGWHSLKTRQLNMFTLIAMGIGVA
                     WAYSVVAVLFPGIFPLAFRSQFGTVAVYFEAAAVITTLVLLGQVLELKAREQTGSAIR
                     ALLKLAPESAHRIKEDGSEEEVSLDKVHVGDKLRVRPGEKIPVDGEVLEGRSFIDESM
                     VTGEPIPVTKEAGAKVIGATINQTGSFIMKAMHVGSDTMLARIVQMVSEAQRSRAPIQ
                     RLADTVSGWFVPAVILVAVLSFILWALFGPQPAFSYGLIAAVSVLIIACPCALGLATP
                     MSIMVGVGKGAQSGVLIKNAESLERMEKVNTLVVDKTGTLTEGRPKLTRIVTDDEFDE
                     EAILTFAASIEHQSEHPLATAIVMAAKEKQLSLGTVEDFEAPTGKGVIGKVNGHRIAV
                     GNARLMQEYGSDKLALFEKADELRSKGASVMFMAVDGKTVALLVVEDPIKSTTPETIR
                     ELQQSGIEMVMLTGDSKKTAEAVAGTLGIKKVVAEIMPEDKSRIVSELRDKGLVVAMA
                     GDGVNDAPALAKADIGIAMGTGTDVAIESAGITLLHGDLRGIVKARRLSEMTMSNIRQ
                     NLFFAFIYNVLGVPLAAGVLYPLTGLLLSPIIAAAAMALSSVSVIVNALRLRRIRL"
     gene            15304..16185
                     /locus_tag="U6309_000076"
     CDS             15304..16185
                     /locus_tag="U6309_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEN5503548.1"
                     /translation="MVFPAIMIVVGIVFLIGGAYLIKSVLQQTSHYRWQWLVYLVFIG
                     AFILSYSASIFYGLLVDPHCLNYFYGSVFLLGATYVYLSCRLMTKTIQKMEVMDDLKN
                     RYELLRHHVKYDSLTGCHNRETLFEMLNTRFQQIKSSDGWVIVLFIDMDKFKAVNDRY
                     GHDVGDNTLAAFGQLLRQRLRKDDIVARYGGDEFIAVMEHISLDEAKKIAENLITVAK
                     QSFKKGILSKLNLGCSIGITQMNPHSASVNSVIKEADLACYAAKQRKINGSVCVYNPM
                     LATRNDTIDLGIHQIAE"
     gene            16353..16724
                     /locus_tag="U6309_000077"
     CDS             16353..16724
                     /locus_tag="U6309_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HEN5503549.1"
                     /translation="MIHFQMKRTILLMVSVLLVGCSYNAKLTPEGEKVTLLQSGKPSK
                     ACAFLGEVRASDRNGMSQSYQSHDHLIKDELNILRNEAALLGANRVLVTQHQQTYEGN
                     PKNDFVADHWMVGRAYRCKTN"
     gene            16749..18062
                     /locus_tag="U6309_000078"
     CDS             16749..18062
                     /locus_tag="U6309_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEN5503550.1"
                     /translation="MEINSMKWFFGWVMLLLLCSSLATAGNVLTLQEITQIALANNKD
                     LKAARYTVSIAKARLIQAGLWPNPSLNLSNNDDRLFNDEGEYSRSAGFSQAFPISGRI
                     AKQKTVARLDVLKAMAEIREAERQLSSKVANAYYAAVITERRMQQVNYLLRINHELVD
                     VIHNRYHAAEISKLDDNAARIEYLRIGQEKHLLHSFSISQYALLNQLMGRKANSPLSL
                     KRDVITGKKLPELAFLKTLALKNRPDRQAIALSIHRANADRRLAKAERFADWTVGLGV
                     QQDKIVVEEGPPQPADRTLGVTLSVPLPLLNRNQGRILEASHTGTQAMMALRALDLVI
                     ETEIASNYAQLKALQMSLRETQTVSLKLAVENVKLARDSYESGQISFLNVLQVQKQQN
                     DLQTTYLNTLEKYLQVYVALCTAIGPGKTNGFCSYLAYQRNGDSK"
     gene            18059..19030
                     /locus_tag="U6309_000079"
     CDS             18059..19030
                     /locus_tag="U6309_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEN5503551.1"
                     /translation="MRWWQQMRRMALISLFLSLLFPIQGCFAHGEEIEVSEGGAKGPV
                     HLTPAQTKMLDIKVVEATNRPMAQMLGLNGQIQLLPDAQADVSIRISGSVTAIDVNLG
                     DSVKAGQRLATVQSRLVGNPPPSVAVTAPIAGIIDARNINLGQAVEPNTVLFHISNRD
                     KLLVVAQVYEEDLGKVKVGQEVTIHALSYPKKVFAGQVTLIEPNLDSLTRTVNVRITL
                     DNEEKLLKPGMFVRANVILARNEAALAVPNAALLQADNQQFVFVQNGDTYERVDVQVG
                     AVDDDFSEITDGLVPGDLVVTQGNRELYTLWLSGGSKFQSGSHEEPQ"
     gene            19031..22243
                     /locus_tag="U6309_000080"
     CDS             19031..22243
                     /locus_tag="U6309_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEN5503552.1"
                     /translation="MFTRLIAWSLHNRPLILALTIILCLLGGYTLKRMPVDVFPEFAP
                     PQVVVQTQAPGMATQDVETLVTYPLESAINGTPGVASVRSKTSVGLSTITVVFDDKTD
                     IYRNRQLVNERIQQVVNRLPPGVSSPVMLPVTSAVGWLLKYALVSGSASPETLRTISD
                     WTIRPRLLALGGVASVVSLGGEVKQYQVRLIPSRMLAYGITVEEVRQALTTGNQNVPG
                     AFVHQAGTEFVVSTIGRIKTLKDIKKTLIVVRNGVPITIDNVAAVAFGGEIKRGDASY
                     NAEHAVIGTVSKIYGADTLTTTHKVEQALADIKKSLPAGIELITEVFRQANFIESAIH
                     NLTKALIEGAIIVIAVLFLFLMNWRASFITFLSMPVSFIVGILVLHYFGFGINSMTLG
                     GMAIAIGEVVDDGIITVENVVHRLRLNRMSDKPLPAIEIVFDAVLEIRSSVVYATIII
                     SLVFLPIFFLSGIPERIFSPLAIAYIASVLGSLVVSITMVPALCYLLLVRKSEKQQEN
                     KVVMHALSQEERYHLTEEEAAKQSQAETRFVIWLKKHFLMGLNWSLDHCKTVVTIAIG
                     AFVFALALLPFFGTSFLPEFHEGNFIIVMSTLPGTSLDESMRLGNQVSKTLLKYPQVI
                     SIAQRAGRSELDEDALPPNISEFDVRLDFGKDKSISPDELLQRIRADLANIPGALFNV
                     GQFIAHRMDEVLSGVRAQVAVKLFGDNLATLNELGQSVEALLKTVPGIVDVNKEQQIN
                     VPQLVITIDREKAARYGINVGQISEDVQVLLNGVTVSSILEGQRTFDLYLRMSETGRD
                     SVKAIQNMLIDAHGTENQKTKIPLRAVADIRLEPQPFAINRENVQRVLVIAFNVQERD
                     LGSVIQEVQQRVKEKVTLPSGYFIQYGGQFESQQQASRVIILFGALAVFIMLILLHKA
                     FGTFREALLVMFNLPLALIGGVISLFLAGANMSVAAMIGFITLFGIAARNGIILVSHY
                     NQLRVQGQPREQVVIQGTLDRLVPVLMTAATAALGLIPLLWGSPAGKELERPLAQVLL
                     GGLFTSTVLNMFVVPTVYNAIEVWREQRMQFNKTEQGETT"
     gene            22240..22692
                     /locus_tag="U6309_000081"
     CDS             22240..22692
                     /locus_tag="U6309_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEN5503553.1"
                     /translation="MKHVTLKQKSHFWLGLITSVLLVCGYSQAMADSTEESTQSIPKT
                     IPAIWEAVDKHAASINQVLSSDDLTTIHQHAFAIRDLVNALPALSKDLSEEQKKTLQQ
                     NLSYVSQLATRLDKSGDANDKKGTQVNWQKLQKVLAQLRSLYPSDAPN"
     gene            22974..24029
                     /locus_tag="U6309_000082"
     CDS             22974..24029
                     /locus_tag="U6309_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5503554.1"
                     /translation="MTQVMKENNRVNASLTALSVMSSYIQSINKPLIPSEFYIELCLA
                     QHIISSVVDFESYGCSYGDENSLKKLLHYLQHNFFNKTVTPGYHSMILARKLMLKHKV
                     EMGILSNGVRQVIFLGGGYDVRAFISSIKFPNVQFYELDRGQTRQIKLKALLNIPKEI
                     GIRQTPVDIRINHTHIFNENFFCVDCDFLEDNLEEVLCANGFKKGSKTLVIAEGLTMY
                     LNEKGVMQLLAGLANLLEEHDEFILSFIPKIINSFSEDMPIESRELHCFSLPPDEVSR
                     FVTAYGFEVIHKNLFKDLLMLMGDINNAKKHENNLILKQEHYYTLRKCTHLLKASSKT
                     IHEIPDYQFSIPFNCCA"
     gene            complement(24066..24521)
                     /locus_tag="U6309_000083"
     CDS             complement(24066..24521)
                     /locus_tag="U6309_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503555.1"
                     /translation="MTTPIDKLLEYKNTQVLNRYKKDYPNNKLKAEEAFPELLKYLWI
                     SQKHKEDLCHFPENDELKFSCGIYPDMLDIDDMWHTFLLFTKDYMAFCDQFFGKYVHH
                     TPASDNEPIPNNEFEVDFSRYLFYIYDHLGEETVKKWFGELLEEEVDMA"
     gene            complement(24987..25271)
                     /locus_tag="U6309_000084"
     CDS             complement(24987..25271)
                     /locus_tag="U6309_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN5503556.1"
                     /translation="MTPGSKLYQIEYIEDVVKNDMPSLSTSAKKLIKKAIEERLMADP
                     IGFGKPLRYSLKGHRRLRVSDYRIVYRIEAETNTVVIIAIKHRKEVYDDF"
     gene            complement(25255..25476)
                     /locus_tag="U6309_000085"
     CDS             complement(25255..25476)
                     /locus_tag="U6309_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503557.1"
                     /translation="MPTKNPRINITFEESTAGLLAYLAELEHKSISGLAKELIMEALE
                     RREDKVLSAIAEFRDHATVKRVKHDDAWK"
     gene            complement(25696..28812)
                     /locus_tag="U6309_000086"
     CDS             complement(25696..28812)
                     /locus_tag="U6309_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEN5503558.1"
                     /translation="MIASIIRWSINNRFLVILVTVMMIVGGLYTINNTVVDAIPDLSD
                     VQVIIKTEFPGQAPQVVEDQVTYPLTTAMLAVPGAVTVRGESGFGVSYVYIIFKDGVD
                     LYWARSRVLEYLSQIANQLPPGAKVALGPDATGVGWVYEYALVDRTGQHDLSQLTSLQ
                     NWVLKFELQKVPGVAEVATAGGMVKQYQITLDPNRLRAYGLTLDQVKQAVQRGNREAG
                     GATIEMGEAEYMIRAKGYLQSIQDIEQIPVGLGKNGIPILLKEIAHIQLGPQIRRGMT
                     ELNGEGEVVGGIIVMRSGQNAMQTIASVRAKLNQLQASLPKGVEIITTYDRSSLIQRA
                     INTLRDKLIEEFIVVSLICVVFLFHFRSSLIIVISLPIAILLALIIMHVQGINANIMS
                     LGGIAIAIGAMVDAAIVMIENAHKHIEHNTITSENRWPMMQQAALEVGPPLFFSLLII
                     TVSFLPVFTLQAQEGRLFAPLAYTKTYAMAAAAALSITLVPVLMGYLIRGKIKAEQEN
                     PINRALMAVYRPILQASLKTPKTTLTITAFIMLIGFWPVSYLGGEFMPELDEGDLLYM
                     PTTFPGISAGKAQQLLQQTDKLIATVPEVKTVFGKIGRAETATDPAPLSMIETTIQFK
                     PKNEWRPGMTMNKLRDELESGLKLPGLSNAWVMPIKTRIDMLATGIKTPVGIKVAGPD
                     LKTIQSIGQQIETVLKKVPGTTSVFAERVASSRYVTVDINRVKAARYGLNIEDIQQIV
                     ETAVGGMNVTQTIEGRERYPVNLRYPREIRDSLENLRSLPIVTPTGATIPLSEVATVT
                     ITEGPDMIRSENARLNGWVYVDIAGRDLGSYVKEAQNIIRQQVKLPAGYSLAWSGQYE
                     YLERAKERLSLVVPLTIAIIAFLLYLNFRRLGEVIIILATLPLALIGGIWLLYLLGYH
                     LSVAVGVGFIALAGVAAETGVIMLVFLNQALEKQQRQAKQEQRALTKDNVYTAVVEGA
                     LLRLRPKIMTVTAIIGGLLPIMLLSGTGSEVMRRIAAPMVGGMASATILTLIVIPVVY
                     LLWQRKKY"
     gene            complement(28809..30077)
                     /locus_tag="U6309_000087"
     CDS             complement(28809..30077)
                     /locus_tag="U6309_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEN5503559.1"
                     /translation="MNKKLVLIIVISIILGVFLGRWTTNIITGKASQEATNKKPLYWI
                     DPMEPMIHYPGPGKSRMGMELVPVYPDDNQEKGEKATVQISPSVVNNLGVRTVPVIQT
                     TLARHIDTVGFVEPNENQISHIHTYADGWVKNLVVKAVGDSVKKGQLLLQYYSPQLVT
                     AQEEYLIALEGNNQSLITASYKRLQALHISEQQIEDIKKNHQSNQLVAVYAPQDGIVA
                     ALNIREGMRVTPDVEIMSLVDLANVWMIAQIFEEQANWVKIGDQAEARISAFPQKQWK
                     GRVEYIYPRLEPMTRTVKVRFRFDNPDNQLKPNMYASISILVNPKSNVLSIPLEALIR
                     SPQGDRVIVALGKGRFQVRQVKAGMESGDRVEILSGLKVGENVVVSGQFLLDSESNLK
                     AGLERLETPEENEKAKSSGMKSTNPKGQKP"
     gene            complement(30074..31345)
                     /locus_tag="U6309_000088"
     CDS             complement(30074..31345)
                     /locus_tag="U6309_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEN5503560.1"
                     /translation="MKTTCFLQGIMTMICLCWGGLAFAIANTPLTLTEAEHLALATSP
                     ELKRLQANSRALQQQAVAVGQLSDPQLLAGTINVPTDTFSFTQDMMTMVEVGLQQQFP
                     RGHSLKMKSQQTQALAKVELKKAFDQVITLLRNVRETWLDLYYWTKALQVLHANRLLY
                     KDLLKVTQSQYSNGKINQSDVIQVELELSRLNDQAIQIQQQLDILRAQLGRWIGINPV
                     NRPLALSMPAWSRPPPLNQLKARLPKHPLLQADASNVKASRYEVAYAKEQYKPGWLLG
                     VSYGFRQGVMPDRMPRSDMLTAQVTMDLPVFTANRQDKQLNASYNRLNASHFERQTHY
                     RDLLQALSAQYATWQRLSEREVIYEKQLLPEAKQNAKAALLAYQSATTELTTVLRAYS
                     SQLTIQLEQVQIQVERLKSRATLLYLEGVAL"
     gene            complement(31774..32085)
                     /locus_tag="U6309_000089"
     CDS             complement(31774..32085)
                     /locus_tag="U6309_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503561.1"
                     /translation="MKKINFLLYLLTMLAISFVTLGHGTTEATDTTNQTKHLYRGSSI
                     SLEELAQVADLSSARDQKGNPVTLEEESYQVGSFTKGTVTIIPKSGKADDVITLDLKP
                     N"
     gene            32322..34001
                     /locus_tag="U6309_000090"
     CDS             32322..34001
                     /locus_tag="U6309_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HEN5503562.1"
                     /translation="MGKENTDSPYQMGHDFFSYQLDCWQRSILFWNTLRERANNMMEH
                     EQQGLPPLLNFKYEFVLDGKSLEPKTNYALVKILEVGDVCFEECFDPHAHPVIIVDPR
                     SGHGPGIGGFKRDSEVGIALHSGHPVYFVIFYPNPVSHQTLADVLMTLRHFVEKVQAW
                     HEGKAPILYGNCQAGWMLALLVSDCVGSVGLTVMNGSPVSYWSNSEEEANPMQLLGGL
                     LGGSWSARFLSDLKEGIFDGAWLVSNFELLNPTTAIWDKYYGLFDEIDGERERFLEFE
                     RWWNGFYQFSQEEIMATVNNLFIGNQLERGEMRIHKGCVYDLKRIQSPLVLFASQGDQ
                     ITPPRQALHWIRTIYPTTQALKKAKQRVVYLLHPSVGHLGIFVSAKVVRLHHRAILEH
                     SDAIEQLQPGLYEMIIVNPTGDPDCSKEQYHVRFEARELTELCTTSSTQPFDKVRQTS
                     EANDSIYQKVIQPSVQSLSNPFLSWWLEKTHPMRLSRYVFSEKVNPLMKAIELLSPPI
                     QANRRMATQSNCFKKIEHGVAQMMRDSLESSRIMRNEVMTNWFEALYKDPD"
     gene            complement(34014..34169)
                     /locus_tag="U6309_000091"
     CDS             complement(34014..34169)
                     /locus_tag="U6309_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503563.1"
                     /translation="MNAKDCFAENDHEEKIRETKSLFDEKEVHEARDLENEMDLDEEF
                     DAIRSMN"
     gene            34366..34893
                     /locus_tag="U6309_000092"
     CDS             34366..34893
                     /locus_tag="U6309_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEN5503564.1"
                     /translation="MLVPQTGIRNASTMIEIIPNWHPIFVHFTVALFSVSVILYALIY
                     FASNTRWKINPFIVELEIVARWCLWLAALSTIATVSAGFYAFYTVKHGAMVHAVKVIH
                     RNWALTTSSAIVLVAFWMVWRYIKHQKPTLVFLMALLFVQVLLLTTAWYGAELVYRHG
                     YGVLPVTAEKTVSPH"
     gene            34920..38006
                     /locus_tag="U6309_000093"
     CDS             34920..38006
                     /locus_tag="U6309_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEN5503565.1"
                     /translation="MLQKDLRCLIRNYCFIFSFLLLNSSALFAQHEQHGASTQPLPSP
                     KTTSSQSKPAQYKMKQPIAKSQPVRTVKPLTMTGGAKRTVNLVVAYKTVNFAGKLRRA
                     IAVNGQIPAPTLHFKEGDDVTINVYNHLDEGTSIHWHGLLVPWQMDGVDGVSQKPIPP
                     GGVFHYRFKLHQRGTYWYHAHAKVQEQEGLYGTFIIDPPNPPSYHYTKDYVVVLSDWS
                     NTRAEQVLANLKKDGDYYGPRFPLQPSLMKFLHDYRKASPEERKKLIADYKMMQQMRM
                     SIYDLSDVAYDAYLLNGHPKSHPWTAPVKVGDRVRLRFIGAGASTIYRVKIPDAKVEM
                     VHIQGNDVSPYPIEDFWIAPGETYDILVTIQKNKPYIIYAESIDTLGKAYGALVTHPN
                     QVVNYQHVTPFPEPLPVTREMMANMMMSMNGGGMGGNQQHALMNKKTSSTAMKPSMTM
                     PSHSQAMSSQSANHSMSSKTIQKKMDKASNHSAHTSSMKSKSSSTSMNKSMDMPGMNH
                     GAMSQNKTSNSAQMKSDSSSTSMNKSMAMPGMNHGAMSQSKTSNSAQIKSDSSSTSMN
                     KSMAMPDMNHSAMGQNKTSNSANDTSMNRNMKSDRPMEQGMSMNGSMNMKMPIEPTII
                     GDKIEPPDSAKATTLGTKYQELKGAVKTNNPNKPVDGIIKMELFGYMDRYIWFINGLP
                     EYKAKPILIEPGKRYRIIFTNNSMMRHPMHIHGHWFILRNGHGAYDPLLHTIEVPPGA
                     TAVADFDTEASGQWFFHCHHLLHMTAGMARVFQYTTIIEIANGTRKPDNYAYQQAYIN
                     RPVVREDEVMPLDASLIKHPAGHHQGFYRSSYIELGEDPFHNAQEMTFRGLYGPDYNK
                     LQLYTEDAEIYKGTVENADIDVFYWHLISQFWAVKGGVNYFYRPGGPYWQPGIGIEGL
                     MPWYIDTNIRTYYRDGSVKFDIQLARANQLTNNFFFLTGIRSILATHTVVKNEIGNGL
                     NQMRYILRPYYRLKPGLNIFSEYEHDVEYGALKRILRSQGEPTTQDTITLGVAVLF"
     gene            complement(38245..38706)
                     /locus_tag="U6309_000094"
     CDS             complement(38245..38706)
                     /locus_tag="U6309_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HEN5503566.1"
                     /translation="MKIVYLIISFMILLLNSGCGGLRQVNADAKNVNLYFSYPTGNCR
                     FLGIIKNPNVHEVVDIRTSLKDLEKDDNNFLKNEGAKLGANVIVLVTHTPSEAHKRYV
                     RGSKNLTTINIHSVIANAYYCPHVKVDQLQQRNLEIKQTPLFENDDLQINH"
     gene            complement(39272..39883)
                     /locus_tag="U6309_000095"
     CDS             complement(39272..39883)
                     /locus_tag="U6309_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN5503567.1"
                     /translation="MERLLIRVIEYNGFFTGIAKIYSTKKWSRKNLNHEILASSKHIE
                     HHCAHFNQGGATMKKRIIPSVCSGFILSCMTQIAIADDIPTRSLPMSQILQHLLSKGY
                     HTIQEVEFDDGLYEVEALDEQGNQIILRMNPQTGDLIKNQNTQKYPISLLEAAQKIEG
                     AGYHSIYKIESHGSQYEVKALGKEDTIVKLRVDANTGKINRDE"
     gene            39984..40157
                     /locus_tag="U6309_000096"
     CDS             39984..40157
                     /locus_tag="U6309_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503568.1"
                     /translation="MKHFTLVKKLMEVLLVCLSFLLFSCSASVSKEDSKSNTPVKKRF
                     GGYHPHVHGRGTR"
     gene            complement(40192..40701)
                     /locus_tag="U6309_000097"
     CDS             complement(40192..40701)
                     /locus_tag="U6309_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN5503569.1"
                     /translation="MPGKIDLAIAAITITDERQQAFLFSLPYLASEARFVTTKNNSLK
                     SLKKLTHKKTGIIRDSLFKQLITQKFKSNSIKEYPSTDKLITALNQYEVDVLLMDDFS
                     ADYWIANNNRLFKGVGDTIPIGIGYGIMAKKNSMALINKMNQALKQIERNGIYLSLYN
                     KYFHTIPMH"
     gene            41085..42335
                     /locus_tag="U6309_000098"
     CDS             41085..42335
                     /locus_tag="U6309_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEN5503570.1"
                     /translation="MRYFSRLVIFMMMYFVVCEVLAATPFTFKQALDTAYRNNPELQA
                     EMDKARAMRGYFIQSGLYPNPQLTLTAENFGGSGAYSSYESAETTFSVTQPIPLGHRL
                     QYLKKATYADYLASLASIRVQQTALYMAVGVAYVDALYAGQWHQVTKKLTRLNQDIVA
                     AIERRVKAGAGAELDLRLAQVRLGEAKIQENKAARDALLQRAKLVRLLGDGLRVDRPL
                     VDKGLPGVSVNWTELVKKLPQSPQLLQMQLQLQAKRKTITAVKKSVWPDLNIQLGGRH
                     FSDDGSNAAVMSAFAEVPVYNRNQGKIMTAEAQYTQTAHEFQGTRLDVRQSVYAAFLQ
                     AQQNHYEANLVTDSLLPLARKAIKLAQEGYQMGRYTYIELSTALNSLYEEERHYQQAH
                     ADYHKALIQMTGLLGLDNSKKESK"
     gene            42332..43585
                     /locus_tag="U6309_000099"
     CDS             42332..43585
                     /locus_tag="U6309_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEN5503571.1"
                     /translation="MTFLKPFKPSILLLILWFVMGVFSWAGEGSHAMEEGNHQEIMEK
                     GPQGGRLFKEGDVALELLIFERGMPPHFRGFVYEKGELIIPTKARLVVYLTRFNGKKE
                     TITFIPVENFLQSNEVIGEPHSFDVTIQLELKGKRLSWHYSTYEGRIKIAPAILKAAD
                     IQTSVAESQTIKTQLKVVGKIAPNRDTLAPIYPRYSGIIKALTKNLGDEVTKGEVLVT
                     IESNESLQNYNITAPITGTIVQKYATNGEQAQNTKPIYEVANLTTVWADFTLYRKETP
                     RVKQGMEVVVTGDEGKPKSTSVISYIAPLGIEDSQTTLARAVLSNEERLWLPGMYVNG
                     AITIKEKTVPVAVLLSAIQRLNDKDMIFVRQGDYFEATPVILGEKDEQWVEVVSGLDV
                     GQPYVSKNSFFMKAELGKEGASHEH"
     gene            43595..46753
                     /locus_tag="U6309_000100"
     CDS             43595..46753
                     /locus_tag="U6309_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEN5503572.1"
                     /translation="MLEKIIRFSLKHRWFVLLFTAVIAALGVYNFQRLPIDAVPDITN
                     VQVQINTQASGYSPFEVEQRITFPIELAMSGLPSLDYTRSLSRYGLSQVTVVFKDGTN
                     IYFARQLINERLQEVKDKLPPEAETTLGPISTGLGEIFMYTVTNKTNVPASQRYNPME
                     LRTIQDWIIKPQLRNVEGVAEVNTIGGYEKQFHITPDPMKLVRYRLSLNDIVEALERN
                     NANVGAGYIESNGEQHLIRVPGQVKNIADIENIVIASFEGTPVRIRDVAEVALGKELR
                     TGAATENSKEVVLGTVFMLMGENSRTVSERVAAKMKEINKTLPEGVEAVTVYNRTLLV
                     NATINTVKNNLLEGALLVCVILFLFLGNVRAALITAMVIPLSMLLTITGMVTNKISAN
                     LMSLGALDFGLIVDGAVIIVENCIKHLGEQQHALYRVLNLEERLKVIAYATTEVIRPS
                     IFGVFIITVVYLPILTLTGVEGKMFLPMAETVIIALLASMLFALTFVPAAVAIFLRGR
                     VQEKENWLVHYLSLGYARVLRRCFHARFLVISAAVVLVVVSLLVAFRLGGEFIPSLDE
                     GDIAMHAMRIPGTSLTQAITMQHLVEERVSQFPEVKRVFAKLGTAEVATDPMPPNVAD
                     TFIILKPRKDWPNPKKTKQELVQEMEQAVKQIPGNNYEFTQPIQMRFNELISGVRSDV
                     AVKVFGDNMDTLLQVAEAISAQLKKVPGAADVKVEQVSGLPLLTVAINRDVLARYGLQ
                     IGAVQDAIVIATGGKRGGELFEGDKRFDLVVRLPEALRSNPNVLRQVFIPLPPSKDNE
                     RHFIPLSEVATLVRSESPNQISRESGKRRVVVSANVRNRDLSSFVNEAKQRIEQNVKI
                     PTGYWVTWGGQFEQLQSASQRLQIVVPITLLGIFLLLFMSFGRVRDALLVFTGIPLAL
                     TGGVFALWFRGIPLSISAGVGFIALSGVAVLNGLVMITFINKLREQKQVYLKDAVLQG
                     SLARLRPVLMTALVASLGFVPMALATGTGSEVQRPLATVVIGGIISSTFLTLLVLPGL
                     YYVFHERRYRNKLPIKKAGEDT"
     gene            complement(46791..46991)
                     /locus_tag="U6309_000101"
     CDS             complement(46791..46991)
                     /locus_tag="U6309_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503573.1"
                     /translation="MLDWASHNFLITAGIIFSIGLVLIVLAKGSSIHRMKLIANIGFY
                     LAFVALVVFVIVLFLDVLPRKP"
     gene            47155..47451
                     /locus_tag="U6309_000102"
     CDS             47155..47451
                     /locus_tag="U6309_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN5503574.1"
                     /translation="MLTQDQIIKLSDILHLMGEVNRLKLLIECLKGPKSVSDLAEQLQ
                     LSVPLTSHHLSLLRSARLLMANREGKHIYYSIYDAHVRCILEDMLKHFTEETEN"
     gene            47584..49725
                     /gene="cadA"
                     /locus_tag="U6309_000103"
     CDS             47584..49725
                     /gene="cadA"
                     /locus_tag="U6309_000103"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN5503575.1"
                     /translation="MTAPLTLTTFFVAGLDCQAEEQLIRRQLQAIPEVEKMDFNFIAE
                     EVTVHHRLPTIEPIQQQIEALGMSVRAKQSTPSDKVQEKLKFDASWQILALSGLLALC
                     SEIAAYLLATEQSLWAIIPALLAIILSGPPTFKKGWLALRTKAMNINSLMLIAITGAI
                     FIGDWPEAAMVTVLFALAERIERYSLDKARLAIRSLMQIVPDVAQVKTANGQWQTLLV
                     EEVPLGAVFRVKPGERIPLDGEVVSGQSTVNQAPITGESMPVSKQIGDSVFAGSLNEH
                     GAFEAKVSKASGDTLLAKIGKAIEQAQAERAPTQRFVDEFARYYTPIMVLIAVLVALI
                     PPFVLGYSFYDWIYKALTLLVIACPCALVISTPVTVVSGLAASAKHGLLIKGGSYLET
                     GYRLQLIALDKTGTLTEGKPVVTDFITYDKSQTNEHLLLLAASLDSHSEHPVAHALVA
                     YWQQEHPDKSLLSVERFSALPGRGVKGAIQGELYYVGNHQLAEDNKVCCLEVEQELKR
                     LEEEGKTTVILSNSTTALAIFAVADTLRANSREAIESLHRQRIKTAMLTGDNALTAQA
                     IAKQVGIDEIQANILPTEKMQAINKLLEQYQAVGMVGDGINDAPALAKATISFAMGKG
                     TDTALETADVVLMNDNLAMLPLYIDLSRKTVRILWQNISLSLVIKTVFFILALGGMAT
                     LWMAVFADMGASLIVVMNGLRLLNSNKTKNH"
     gene            complement(50264..50839)
                     /locus_tag="U6309_000104"
     CDS             complement(50264..50839)
                     /locus_tag="U6309_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN5503576.1"
                     /translation="MKPPKKVKNSERRAREYLTPAEVDKLIASAKQLGRHGLRDSTMI
                     LIAYRHELRVSELISLRWSQIDLKQGLLHVIRKKNGIPASHPLFGPEIRALRQLKRDY
                     PETDYVFMTERKAPITADTFRKIIARAGEKAELGLATYPHMLRHSTGFKLANDGRDTR
                     SIQHYLGHKNIQHTVRYTEIASVKFKEFWDD"
     gene            51019..54037
                     /locus_tag="U6309_000105"
                     /pseudo
     CDS             51019..54037
                     /locus_tag="U6309_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            54330..54626
                     /locus_tag="U6309_000106"
     CDS             54330..54626
                     /locus_tag="U6309_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HEN5503577.1"
                     /translation="MSPAFGHHPKSPKPQKEKGFWSSPSGLMAIIIIGIIGYYLIVEH
                     GAHIASFLGASPLVLLVLLCPLMHLFMHRGHGGHGENHHHKSDDEDNDSSENKD"
     gene            54633..55298
                     /locus_tag="U6309_000107"
     CDS             54633..55298
                     /locus_tag="U6309_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEN5503578.1"
                     /translation="MEHTSYAYGLWLLVIVNSAIFILFAYSFTTTFKTRRDWRAFGAF
                     SAFVIAYFTEMYGFPLTIYLLSGWLSKYYPGMDLYGHDSGHLLHTLLGLKGDPHFDVF
                     HILSFVFIIGGLWMIASAWGTLYRAQKAHQLATSGLYAKIRHPQYDGFILVMIGFLLQ
                     WPTILTLIMFPILVYMYVRLARREEKEVLAEFGEEYKHYAAVTPGFIPRISKQQDSKG
                     GAP"
     gene            55295..55795
                     /locus_tag="U6309_000108"
     CDS             55295..55795
                     /locus_tag="U6309_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503579.1"
                     /translation="MMFMFHSAEMLGLIAIALGIMLVVWALRNEGKSIGLAKIFGWLI
                     VIIGILGELCSTYYAIKYWYAGYFQMPMMSGEAFLFHVAQMLSLIAIALGIILIAWAS
                     HNEGRGISLAKVFGWFIVLFAILGMTCSAYYATVYWYKGYIQTPAGMPMLLQHQIPMQ
                     KQSPNQ"
     gene            complement(55863..56684)
                     /locus_tag="U6309_000109"
     CDS             complement(55863..56684)
                     /locus_tag="U6309_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503580.1"
                     /translation="MKLRWATLAALTVIAALSFMAYYLSSHGDEVSSLTNVAEWQRMA
                     SPGELSKAHSFLSHNCTACHTPVKGVEAANCIVCHANNESLLKRQPTSFHANIGSCVE
                     CHREHQGRTARITQMDHNALVSLGLQQLRAFSNANDEEEQLIINQVTLFLAQNESNRP
                     TVLVNPHLSPQELTLRCASCHQNKDKHFKLFGDDCSACHETAHWSLSEFRHPSPSSMD
                     CAQCHQAPPSHYMEHFNMISKKVAHEHEARVDQCFLCHQTTLWTDIKGVGFYKHH"
     gene            complement(56681..57442)
                     /locus_tag="U6309_000110"
     CDS             complement(56681..57442)
                     /locus_tag="U6309_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503581.1"
                     /translation="MNAQGRMMVLGLVLLMLITWLGFAVHTSSRFAGSFWGGVFGVSG
                     SILMLIPLLYLFIKRISFLKNWVTHYVSMNTLLSWHIYTGIIGSILVLIHTGHKFNSA
                     LGIALTAMTLIVTLSGFIGRYLMGFMTQEIDEKKTILLGLQKQYQIVAQELQESARSK
                     QNIGKLHLMTARFLSWLLEDTALDGQLIKSAEVRALCLADAISELEYAIRFHEHFKRL
                     FQKWLTCHIITALVLYVLLGLHVWSGIYFGLRWFQ"
     gene            complement(57442..59325)
                     /locus_tag="U6309_000111"
     CDS             complement(57442..59325)
                     /locus_tag="U6309_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEN5503582.1"
                     /translation="MLFQYIKKFFLFLWVQAVSISFLLAMASVSFAQLTAQDHAAHHQ
                     GEKEGEGMSGMMKNIGTPSKELYPSLMSLPTLSPEKRLAIQQKAHQRITSGMALMKGG
                     LNELLLASNNNDYAKMQEALEKLREGIAQYESALAALRALSEGKNPSDIALQWFKREM
                     NLLPSSPQAESVLGGPFFHLTIIALFGLFFLVMIWMYFFKMRRAAALLDRLTKQNAAV
                     SSQTPPQFSKAPEPTPSLTSVEPIKSSQVSKVPSETAPTMPPTCPAHKCPVPQFPVMR
                     SLTEPEEKWEGQLRVCRIFQEAPGIKTYHLASTHEVALPFTYYPGQFITLTALINGKT
                     IRRSYTMASTPTQLHYCAITVKREEQGLFSRYLHDEIKEGDLLEVMGPNGKFTFTGEE
                     AKSIVLICGGVGITPMMSIIRYLTDIGWHNDIYLLYCCRTTSEFLFREELEQLQERYL
                     NLHVYASMLRSEGTIWMGLQGLFTKNIISHLVPDIASHRIHVCGPPAMMEAILGSLKE
                     LKVPADLILTEAFGPEKKPEIAQEDLVTIKAETRAMISFRKSEKMVPILPDCTLLEIA
                     EANGIAIDNACRTGQCGLCKVTLLSGEVTMACEDALSKEDKQQGLILACQAKATQNIE
                     VDA"
     gene            59673..60047
                     /locus_tag="U6309_000112"
     CDS             59673..60047
                     /locus_tag="U6309_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503583.1"
                     /translation="MAKQWMVLIGCVVLSLLTTASLAQYRNGVFSVEYSKASPIKNIP
                     LKKATLIIKIYYYGYPKGHFSVVTDEKQHFIMGYDDKYQIALELIAISGQEQYKALCR
                     GESKPGQLKLIVVCNPYKKKTL"
     gene            complement(60079..60948)
                     /locus_tag="U6309_000113"
     CDS             complement(60079..60948)
                     /locus_tag="U6309_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEN5503584.1"
                     /translation="MTKRTKLKEHVHTLEEIGHIMTAMKNLSFIELGKITQCLSMQDN
                     VIKTIREVSHDFLSFYPMPPMNQQENPPLVSILIGSERGFCGSFNDNVLHQLDAHNEQ
                     HSEWEPALVLVGHKLALKMANDSRVMATIDGPNAIEEIPAVISNVLHTLERALLQRNK
                     TWHSWQWTVLFNEEEHNQIQVKTWQPFKELDISPAPPFSVPPVLNGSKDQFFADLVEH
                     YLLAMCYAIFYQSFFAENHQRLFHLNQALDRLENKKNALNHRLNLMRQEEITEEIQNI
                     LQSAQAIIGHEFT"
     gene            complement(61006..62478)
                     /locus_tag="U6309_000114"
     CDS             complement(61006..62478)
                     /locus_tag="U6309_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEN5503585.1"
                     /translation="MNWSNTPSFLEKQRQRLERYQFQIKVSEQGRVVSVGDGIIWIKG
                     LPGAAIDEILISEDECCIAMVFHLTEELVGAVMLVQTKKLKAGTPIFPLKRVLSIPVG
                     DKLLGRVIDPLGHPLDGGEIPPHEEQGLLDRLSPPILHRDFVNRPLYTGNKMLDNLIP
                     IGKGQRELLIGDNGLGKSALALDIVMNQKDKKVYCVYVLIGQKRSTVSSTIQLLKEAN
                     ALDYTTVVVAQATALPGLLYLAPFAGCAIAEHWMKKGLDALVIYDDLSAHANSYRELS
                     LLLRRPPGREAFPADIFYLHSRLLERSTCLSPALGGGSMTALPIIETKEGEMATYIPT
                     NLISITDGQIFFDESLFSSGFLPAIDITKSVSRVGGKAQHPQIKKESGRMKLDYLQFL
                     DLELFTRFGAKLDAKMQKQIQKGRVLREILKQERFSPLPIEFQLAWLIAYNEGFFDEL
                     NLEDIPKMLKKIEEEIKQSTLSLGSPREQWKKAIKEWLMA"
     gene            complement(62465..63208)
                     /locus_tag="U6309_000115"
     CDS             complement(62465..63208)
                     /locus_tag="U6309_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEN5503586.1"
                     /translation="MELSWTTFSLELINFLILIWILKRFLYAPIQKTILERKKRVQEQ
                     LETAQTLHRQATQLQTTYEHRLADWQQEKATLQNEWHEAMERWKSEERLQFEKQLHQE
                     KEQIFSHEMQKAAAIIENNAKEAFLLAGKFAEQLLMPFADAHLEEKIIKKTIEELHHI
                     PTEQWQWLHTVPEEETVSIQTAYPIQEHQKQDLLQVIEQLSPKKITVSFTENPKLLAG
                     LTLQMGPMCLQANLRDELKFFIETKNELV"
     gene            complement(63214..63489)
                     /locus_tag="U6309_000116"
     CDS             complement(63214..63489)
                     /locus_tag="U6309_000116"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEN5503587.1"
                     /translation="MNDISWFSLASTVIAAIAIAIGTIGPALAMGRAISHALDALARQ
                     PEAEKSITRTLFIGLAMIESLAIYCLVIVLIILFRNPLLSYFVTQQG"
     gene            complement(63486..64178)
                     /locus_tag="U6309_000117"
     CDS             complement(63486..64178)
                     /locus_tag="U6309_000117"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEN5503588.1"
                     /translation="MGEEGFLAHFAFSIGGLPITQSVLTTWFIMISLFIMAWSTTYKC
                     SLLQPSTYQLIWEGVLSTMYDAIKEVLPDHVELIFPFVATLWIFILVSNLIGVIPGFY
                     SPTADLSVTASLAIMTFLSVHWFGIRAEGWREYLKHYIKPTPFLLPFHLISEISRTLA
                     LAVRLFGNIMSLQLTALIVLMIAGFLVPIPILILHIIEAIIQAYIFGMLALIYIAGGI
                     QAHELKSQGESL"
     gene            complement(64181..64459)
                     /locus_tag="U6309_000118"
     CDS             complement(64181..64459)
                     /locus_tag="U6309_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEN5503589.1"
                     /translation="MEFKNSNKELEQQVKRNVRKINKAKKGKSTLLAQTVYLGTLGFV
                     FVLPIIAGAYLGVWLDEKIKGYSMSWTINLILLGVIIGAINVYLLIKD"
     gene            complement(64449..64859)
                     /locus_tag="U6309_000119"
     CDS             complement(64449..64859)
                     /locus_tag="U6309_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEN5503590.1"
                     /translation="MELFTIHLKSATQYEKIDKVISFVGEDASGQFGILAHHSRMMTC
                     LKFGLAWFRDENQETEYLALPGAVVYFIDNQLHIATRHYVRHQEYQAIQRVLEEELHW
                     EENNLLRTKESVHRLDEEMLKRLWELNRQNSYGI"
     gene            complement(64868..66292)
                     /locus_tag="U6309_000120"
     CDS             complement(64868..66292)
                     /locus_tag="U6309_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEN5503591.1"
                     /translation="MGHEFNWKMMDKNTAVAQTHGTPIGRIIEINGPVVRIHCDVLPP
                     LHQSLKTYTDSDEYILEVCQHLDEHHVRAITLHRASGLQRGLIVYDQGTSLRIPVSKE
                     CLGRLLNIFGEPLDGAPPLETHEYRDVLANFAPLEMTSTQETILETGIKVIDLLCPFV
                     RGCKTGLFGGAGVGKTVLLMELMHAIIQLHQGTSVFAGVGERIREGHELWHEMKSAGV
                     MDKTLMVFGQMDESPGVRFRTGLSALTYAEYLRDTLGHEVLFLVDNIYRFVQAGSEIS
                     GLLGRMPASVGYQPTLMTEIAELEERMTSTAKGAVTSVQAVYVPADDMSDPAVTGIIT
                     HLDSIIVLSRAQAGKGIYPAVDPLASKSQFMDKILLGERHYSIAQAVREHLERYQELE
                     DMISMMGIEELSPKDRAIVLRARKLQRYLSQPFHVTKLQTGMEGKSVSLEHTLTDCES
                     FLRGDYDEFSEEECYMRGAMDKGR"
     gene            complement(66631..67080)
                     /locus_tag="U6309_000121"
     CDS             complement(66631..67080)
                     /locus_tag="U6309_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5503592.1"
                     /translation="MLKRNVFILLTACSLVATPLFAEDMSSGSQMNNTNSMGMQSSNQ
                     QSTQLQDEMKQMQEALKQMQEMHDKLMNAKTTEEKKSLMQTQMPMMEEGMHAMQKGMK
                     MMKSMQDDAAKQPETMEQRTKMMQMMMQMMQMMMDRQMMGCPMMKNQ"
     gene            complement(67185..67337)
                     /locus_tag="U6309_000122"
     CDS             complement(67185..67337)
                     /locus_tag="U6309_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503593.1"
                     /translation="MPKQRLIKAVIGIMSAILLLISCAPSTTTNNSHRVSHTYEGHNT
                     GGIGWH"
BASE COUNT        18983 a        13558 c        15008 g        20114 t
ORIGIN      
        1 gaggtaccct aaatgttggt taggatcgtc agcgatggtt ctttgttggg atgacgaaat
       61 ggatgtgata cgtgtcaatt aattagttta atatttcaaa aaactaactc aaattttgaa
      121 tcggtttagg atacgaaaaa agtgagctaa aaacaattca actatcattc aattaattca
      181 aaacaaaggg acaaagtttt cttttgcacg taaatttttt taggtttttt tgaggacaca
      241 tcaagacatt ttaattgagc ttctcaaagg ttattcctag ctaattgttc gtcaaagcat
      301 agaacacttt gttttgactt atccatacca aagagtctat attgtaatta tttcctttaa
      361 aatcaaggat agaaattatc atgaaacaaa gtcattggat ggttttggcg tgttgtttag
      421 tccttactgc gtgcatgagc agtggcacga gtagtaagta cactaaaagt ctcgaagagc
      481 atgtcgctca aggtcatgtt ggccatggtg gcggtggtgg tcgctaacac taaaatcttt
      541 attactcaat cctaaagatt catatgagcg ctcatcctat aaagtctaag aactattaat
      601 ctattcgggc actaaaaatt gagataaaat gatgcgcacc acatcatagt ggacgggcga
      661 atgcaaagga tggctgtcat ggcagaacaa tggcacgaaa aagcacctgc agatatggtg
      721 ttgttgtttc aaaccgactt atctttagga ctatctgaaa aagtagcaga acagcggcta
      781 aaagaagtag gacctaacct gctgagtcaa cagaaaaaaa catccccttt cgtcattttt
      841 ttgcagcaat ttgcaagtgt ggtgatttgg gtacttttag gtgctgttgt tgtgtctttt
      901 ttgttggatg aaaaagccga tgccattgcc attttggcca ttgtcatttt aaatgccatc
      961 atcggatttt ttttggaata tcgcgctgat agggcaatgc ttgcgcttca gcaaatggct
     1021 gctcccaaag caacagtgct tcgcgatggc catgcgaagg tgatagccgc ttctgatatc
     1081 gtaccaggtg atattcttct ttttgaaagc ggtgatttaa tcgctgccga tgctcgcctt
     1141 tttgaattat cagcgcttaa ggttaatgag gcgccactca cgggtgagtc gatacccgtt
     1201 gctaaaaatc tggatgtttg cactgccgag acgcctctag ccgatcgtaa aaacatggtg
     1261 tttatgggca cctctatagc cgatggcacc ggtcgcgcgc ttgtcgtggc gactggtatg
     1321 caaacggaaa tggggcacat tgccaaaatg ctgggtgaag cgtctcgtga tgaaacgcct
     1381 ttgcagaaaa aactcaatca agtgggttct cgtctattgt ggctttgctt tttcattatc
     1441 attgccattt ttggcttagg tcttttacgt aatatctctc tattcagctt attcatgagt
     1501 tcagtcagtc ttgcagtggc tgcaattcct gaaggcttgc ctgccgtagt aacggtggct
     1561 ttagcactcg gcgtgcaacg tatggtacgg cgtgccgtac tggtaaggcg attatcggcg
     1621 gttgaaacac tcggctgttt acaagtcatt tgcacggata aaacaggaac attaaccgtg
     1681 ggtgaaatga ccgcacgtca gcttgtgaca gcaagtgatg tttatagtat tcatggagaa
     1741 ggctataacc tctcgggagg atttactctt caaggtcaag aaattaatgt gtcagaagac
     1801 ccgttgttgc aagctacttt gcatgcgatg gtggcttgca ataacgcgag ttttgagagc
     1861 caagatggac aggtagctac tgtgggagac ccaactgaag tggccctttt ggttgcagca
     1921 gccaaaggag gcgtttggca aggggaactg caagcctctt atcctcgcat caaagagtta
     1981 ccctttagct ccgaacgcaa acgcatgacg gtcgtgtgtc gacaagacca tgaacacatc
     2041 gcttttgtga agggggcacc agaaattatc ttggagcgct gcacccatat tttaaccaaa
     2101 acaggcatta aaaaagtaac gccgaatgat aaggcgcgca tgaaacaatc ctgtgaactc
     2161 atggccagcg aagcattgcg actgttagct tttgcgaagc gccaattaga gcctacaact
     2221 ttagaagaag aggaagagat tgaacataac ctggtgtttt taggtcttat tggtcttcag
     2281 gacccacctc atgcgagtag caaggaatcc atcagtcgct gtaaaaaggc tggtattaaa
     2341 ccagtgatga ttacaggaga ccatcctgat acggcaagag ccattgccaa agagcttggc
     2401 attttggagg caggtgaccg attgctcact ggcaatgaac ttgaaaacat gtcggaggaa
     2461 gaatttaacc attgcgtcaa agacattgct gtctatgctc gtgtgactgc ggagcataaa
     2521 ttaaaaatcg tacgggcctg gaaaaaacaa caaatggtgg tggcgatgac aggcgatggg
     2581 gtgaatgatg cgcctgcttt aagagaagct tcggtgggca ttgccatggg gaaaacaggg
     2641 actgctgtaa ctaaggaagc ctctgacatc atcgtcatgg acaataactt cacctcgatt
     2701 gtggcaggta ttgaagaagg tcgcacgatt tacgataaca ttgccaaaac gcttgcctat
     2761 ttattagcag gcaatagtgg ggagttactg gttgtgtttg tagcactttt aatcggctgg
     2821 ccattgccgt tgcttcctat ccaattatta tggattaact tagtaaccga tggtttgccg
     2881 gccattggct tagctaccga tatgtcagag ccagggattt tgaatcgtcc acccagagcc
     2941 acgcaaaaat ccatgatgga tatggcgttt ttcaaacgag taacctttgt gggttgttta
     3001 aaggctttag taatccttag tgtgtttgct tatgaatatt taatcgataa ggatttaatt
     3061 caagcccaag atgcggcctt ttctgtttta gtaaccgccg agcttttatg ggcctttgga
     3121 gcgcgaagcg acaccaaaaa tatttggcaa gtaggattgt tttccaattt aagactcttt
     3181 tttattgtga gcatcagttt ttccctgcag gtcttgattc atcatatccc ggtattgcgc
     3241 gaattatttg ggatacaacc ggtctcattc acccaatgcc tggtttggat tctattaggc
     3301 atggtgcctt tgttagtgat agaagctcag aaacggctaa ggaaggcacc caatgaagca
     3361 ttttagtgct aaaaaaatga tttggactat tctcttaggt ttctgttgtt ttgcagggcc
     3421 agtatcttat gccaagacac cgcctgcttt atcgcagcta atcaatgagg ccactcaaaa
     3481 caaccctcaa attcgtgcgg cacgcgaccg actactggca gcaatccatg tgattccaca
     3541 ggcaaaagcg ttacccgatc cgaagcttaa tgcagggtac atcaacatgt ctgagaatat
     3601 ccctatggat gtggatccaa gacgcgagca aatgttgggt ggccaacaag aaattccttt
     3661 tcctggcaaa ctcattgtgc gaggaaggat agcaacgctc gaggccaaaa gagcggaagc
     3721 agaataccaa gccactagtt tcgccgtcat tgctgaatta aaacggctct attacgatct
     3781 ttattttgtc aataaatcca tcgagatcgt acaaagaaac caggaacttc ttcatgaaat
     3841 ggaaaagagt tccgaagcca attacagtgt agggaaaaca ccccaacaag atatttatcg
     3901 tgcccaaact gagatttctc gtctactgat gcgtttagtc atcttaaagc aacaacgggg
     3961 atccctgcag gcggatatca atcgcctttt aaatcgttcc ttagaaatta caatccatac
     4021 accagcagtg cttcctgtaa cgccaatggg gcataattta gagtattttt acacgctcgt
     4081 taagagccga gcaccgcaat taatcatgca gcaacgtgct gttcaaaaag gacgacaagc
     4141 catccgctta agtaaaatgg agtatttccc ggatgtggaa atcgaagggg gacgacttca
     4201 tgatacggga atgcacacca aaggctatca agtgttgtta aaagcaacgg ttccacttta
     4261 tttcatgcaa aagcaaaatc atgccgtgcg cgaatcactt gcgcggtata atgcggatat
     4321 tgaggatttg cagaccacct atcgtatgct ttcctttcaa gtgaaaaatg cctatttgtt
     4381 ggcagagcgc tcggcaaaac tcattcatct gattcaacat accatcattc cacaagccac
     4441 tttaacattt acttcatcgc aagccaccta cggcgtgggt aaagtggatt tcttaacgat
     4501 gctgaataat ttattgacgc tgcaagaaaa tgaattggaa tggcacggtg aacttgctga
     4561 acatgaaaaa gcgatcgcgc aaatcgaaga aagcacaggg acgttcttat gaattttaag
     4621 ttaataatca agagtgcggt tattttgagt ttactttcgt tgttgcaggt aactcaggcg
     4681 caacaggcca tggataatca tggcaagatg gacatgaaac agtccactca aggaaatgag
     4741 attgtgattg atccggctcg tttacaagcc attggcatta cgtcagagcc ggtgcgtcgc
     4801 caagtggttg aaaaaataat ccgtaccgtg gggcgtgttg aagccgatga gcggcgtgtt
     4861 gcccatattc atgtacgctt tgatgggtgg attgagaact tattcattaa ttttaccgga
     4921 gaaaaagtca aagcagacca agctcttttt accgtttaca gtcctgaatt ggtcgccacc
     4981 cagcaagaat atttgctcgc ccttaatgcc caaaaaatat taagcaaaaa tacgacatcg
     5041 agtgcggctc gtggtgcgca gtctgctttt caagcagccc accaaaggtt gttgctgtgg
     5101 gggatttccg aaaaagacat tgagcaatta agacgtacgg gcaaaatcac tcggacgatg
     5161 acgattcatt cccccatcca aggtacggtt cttaaaaaaa tggcgctggt gggcatgcgc
     5221 attgagccag gagatgaact ctataccatt gctgatttat cacgcttgtg ggttttaggg
     5281 gacatttacg aatatgagct tccttatatt cgtcttggtc aaatggctga tttgaccctt
     5341 acctacttgc ccaatgaatt atttaaggcc aagcttgatt ttatctaccc tacgattgac
     5401 atgaaaacac gtacagccaa ggttcgcttt gaagtggaca accagaagga gcaattaaaa
     5461 ccgggcatgt atgtgaatct ggaactcaaa gtacctttgg gtgaacgtct tgtggtccct
     5521 aaaaatgctg ttctactcac aggtgagcgc gcagtggtgt ttatttacca tggcaatggc
     5581 aaaattgaat ggcgggacgt gaccttaggg gttagggcag gggatttgct tgagataatc
     5641 aaaggggtta aagagggcga tcaaattatt acgtctgcca atttcctcat tgactcagag
     5701 agccagttga aagcggccat gggaggtatg caacatcaat agactttctt accgagcctc
     5761 tacaatagga aagaagctct aataaagagg cgtgatttgg ccttggaata aagggatttt
     5821 atggttgaac ggattattga gttttgtgca cgcaatcgtt ttattgtgtt gctttttgtg
     5881 ctcgggtttt ctttcatggg ctatatggcc ttgaagaata cccgtatgga cgcattgcct
     5941 gacatttcag acactcaggt cattgtgtat acgacctgga tgggacgttc gcccgatctc
     6001 atcgaagatc aagtcacgta tcctattgta actgcattgt tgtcagcacc aaaagtagta
     6061 gctgtgcggg gattttctga ttttggcttt tcttatgtct acatcatttt tgaggatggt
     6121 acggatattt attgggcgcg aagtcgggtc ttggaataca tgagtcagct gtctggaaaa
     6181 ttgcccgaag gagtgactcc tcaattgggc cctgatgcga caccggtggg ttggatttat
     6241 caatacggct tggtggatga caatggcaag cataacctgg ctgagttacg cacatttcag
     6301 gattggtatt tgcgctattg gttaagagcg gttcctgggg tgtctgaagt agccagtgtg
     6361 ggaggttttg tcaagcaata ccaagtcaat ttggatccag tcaaagtcct tgcctacaac
     6421 ctatcggttc ctgagattgt tgaaaaaatt cgtatgagta ataacgacac cggtggtcga
     6481 gtcattgagt tttcgggcgt ggagtacatg attcgtggtc gcggatacat taagtctact
     6541 gaggatattg aaaaaattgc cgtgggcacc aatgccaatg gcacaccgat tttattacgt
     6601 gatgtagcga ctgttcagct tggctcggac atgcgccgcg gggtggtcga tttaaatggc
     6661 caaggcgaag ccgtgggtgg cattgtcatc atgcgctttg gtgaagacgt gctgcaagtg
     6721 attggtcgca tcaaggataa attgaaagaa ttagcttcgg caatcccgga agggataaaa
     6781 atggttcctg tgtatgaccg aagtcattta atccgggaag cgatttccac ggccaattgg
     6841 aacttgattg aagaactggt ggtggttggg ttactcatta ttgtgttttt agggcatttt
     6901 cgctcggcat tgattcctat tattactttg ccgctggcga ttttgatttc gtttatcccc
     6961 atttatttct tacatgtcgg actgaatatc atgtccatcg gaggcattat tgtcgccatt
     7021 ggcgatatgg tcgatgcggc catcatcatg gtcgataatg cccataaacg cttagcggat
     7081 tgggaagcaa aaggaagccc aggtgatcgc acccaggtgt taatcgattc ggcgaaagag
     7141 gtggggcctg ccattttttc atcccttttg gtcattgcca tctccttcat gcccgttttt
     7201 accttggaag ctcaagaagg gcggctgttt tcgcccttgg cttatactaa gaatttggcc
     7261 attttcatga gtgctttact tgccatcacc ttaattcctg tcctattgcc tcttttggtg
     7321 cgtggtcgaa ttatccctga acaaaaacat ccgattactc gcttgatgca aaccatgtac
     7381 gcgcccgtgt tgaagcttgc tttgaggtat cgcaagttcg taatgggaat tgccattgta
     7441 ttggctatgg ccaccattcc tctttataaa ctcattggct ctgaattcat gccccctctt
     7501 tatgaaggga cgattttgta tatgccggtt actttaccgg gtatttcggt gacgcaagcg
     7561 acggctttgc ttcaagaaat ggataagaaa ttaaaagcat ttccagaggt ggcacacgtt
     7621 tttggcaaga cgggaagagc cgaaacgtct accgaccctt ctccatttaa tatgatggag
     7681 gtgattgtgg agttaaagcc taaagaattt tggcgcaaag gagtgactta tgaaagcctg
     7741 gtgaaagaga tggatgaagc gttgcaattc ccaggtgtga gtaatgcttg gaccatgccc
     7801 attaaagcgc gtaatgacat gctcaccaca ggaattcgga ctgctgtagg aataaaaatt
     7861 tttggccccg acattaaaaa aattgaagcc attggcaaag aaattgaaat ggtggccaaa
     7921 gaagtaaagg gcaccagtac ggtttatgcc gagcgggtgg ctggaggcta cttccttgat
     7981 tttcaaatta atcgggacca gttggctcgc tatggtttaa ccattatgga cgttaatcgc
     8041 atcattgaat cggccgtggg tggtgaaaac attgccacca cgatcgaggg gcgtgagcgc
     8101 tacccagtga atgtgcgtta tttgcgagaa ttgcgcgatg accccgacaa gctcaatcgt
     8161 attttagtca aaacacccag tggtgcggag gtacccatcg cgcaattggt gacgctaact
     8221 tttcgttcag gacctgccat ggttcgcgat gagaatggca tgttggcggg ttatgtcttc
     8281 attgacatca gcggccgaga cattggcggc tatgtggagg agctaaagca agcagtcaaa
     8341 gtcaaagtga aattgcctcc aggttataca ttggcctggt cgggacaata tgagttcatg
     8401 caacgggttt atgagcgact taaaattttt gtgccactga ccttagctat tatttttgtt
     8461 ttattttact ttaccttccg caccatcact gagactttaa tggtgatgct aggcgttccc
     8521 tttgctttat ttggcggctt tttactgctt tatgtattag ggtataacat gagtattgcg
     8581 gtttgggtag ggatgatagc gcttgcagga gttgctgctg aaaccagtgc tgtgatgctc
     8641 gcttaccttg attccgatta ccatgaacaa aaggaaaaag gggggctcag aaccttgtcc
     8701 gatttgattc agatggtgca acaatgtgca gtctcccgca ttcgtcccat ggctatggcg
     8761 ggtcttgcta acatcattgg cctattaccg gtgatgtggg ctacaggcat tggagccgat
     8821 gtgatgaagc gtcttgccgc tcctatggtc ggtggggttt tttcagcgct actgctgacc
     8881 ttaattgtga ttcctgtggt ttatgtgatg tggcggggcc aaatggattt gaaaaaaaca
     8941 gcagacttaa cggaggcaag atgaaattaa cttttttagg agcaactgag acggttactg
     9001 gttctaaata tcttttgacc gtcggttcta aaaaaatcct gattgattgc ggtctttttc
     9061 aaggctataa agaattacga ttacgcaact gggctccttt acctattgac ccccatgata
     9121 tcgatgcggt gattatcact catgcgcata ttgaccatac gggttatctt cccttgctcg
     9181 tcaaaaatgg attccaaggc aaaatttatg caacaccggg taccaaagca ctgtgttcca
     9241 ttttgttacc cgacagtggt catctgcagg aagaagaagc ccgcttggcc aataagtatg
     9301 gattctccaa acaccaacct gctcttcctc tttatactga aaaagaagct catactgcat
     9361 tgcagtattt tgaaaccatt gattttgata ccccgcatca attattccat gatttcagtt
     9421 ttgtgtatca tcgtgctggt cacattgtgg gtgcggccat ggtcaaaata aaaacccaaa
     9481 aaggttccat tgtatttact ggagacattg gccgtcctta tgatcccgtt atgaaagccc
     9541 ctgccttcat tcaagaaacg gattatctgg tgatggagtc cacctatggt gaccgattgc
     9601 atgatgccac agatcctttg cctcaaatgg ctcaggtgat taatcaaacc gttaaacgag
     9661 ggggctcagt cgttattcct gcgtttgcag tagggcgtgc gcaaagtctt ctttatttta
     9721 tttatgaact gaaacgacaa gggcaaatta ccaaagacct tcctgtcttt ttagacagcc
     9781 ccatggccat tgatgccact catttactct gcacttataa agaagaccac catttaactg
     9841 cagaacaatg tcgtggacta tgccatgttg ccacctatgt gaacacacct gaggagtcca
     9901 aggagattga ccgtcatcac atgccgcaaa tcatcatagc ggccagcggc atggcgcaag
     9961 gcggccgcat tctgcatcat ttaaaagcct ttgcgcctaa tcctaaaaat accctacttt
    10021 ttactggctt tcaggcgggt ggcactcgtg gtgctcgaat tgtgaatggg gagaagcaag
    10081 tcaaaattca cgggagtttt attccgatac aagctaaggt cgtagtcatg agcagtacat
    10141 cggctcatgc cgactacccg gaattgttag aatggctaac tcatttgact aatccgccta
    10201 aaaaggtttt tatcacccat ggggagcctc acagcgccca atcgctaaaa cagaagattg
    10261 agaagcaatt aggtttttct tgcaccattc cttcttatct gcaaacggag gaattgtggt
    10321 gagtaagcaa acctctcatg ggcttcggtt aaaacattta gggattaaaa cctatcacga
    10381 agctattatt tacatgcgcg aggattgtca tgtttgtcat tcggaaggat tcgaagtaca
    10441 gacccgtatc caagtgactt taggtcagcg ctctattata gccactctta atgtggtgac
    10501 ctcagagcta cttcagcctg gtgaggctgg tttgtcggat tacgcctggg agtctttaca
    10561 tgccaaagaa ggcgatgaaa ttcaggtctc ccatccaaaa cccttggaat ccttaagcta
    10621 tgtgcatacc aagatttatg gcaaagaact gtcttatgaa caaatgaagg tcatcattga
    10681 tgatgtgtta agcggtcgtc tttctgatgt acaaatctcc gcttttctag cggcaagtgc
    10741 ctcaggacgt ctaaccagta cggaagtgat gaagctcacc aaagccatga ttgatagtgg
    10801 tgatcgctta tcttggcctt cacctctggt cgttgataaa cattgtgtgg gaggattgcc
    10861 tggaaatcgc accactctga ttgtggttcc catcgtggct gcctttgggt tgatgatacc
    10921 taaaacgtcg tcgcgtgcca ttacatcacc tgcggggact gcggatacca tggagacttt
    10981 agctccagtc cacttaagtc cccaaaagat gcgccaagtg gttgaacaag aaaacggctg
    11041 tatcgtatgg ggtggggctg tgagtttaag tcctgctgat gacgtgctca tccgtgtgga
    11101 acgagctatt gatttagata gtgaagggca attagtagct tccattcttt ctaaaaaaat
    11161 tgcaacaggt gctacacatg cggtcattga tattcccgta ggacctactg ccaaagtcag
    11221 gaatcagtct atggcgctcc ttctgaaaca attgcttgag gaggtaggta acgagttagg
    11281 gcttgttgtc catactttat tgaccgatgg ttcacaaccg gtaggatatg gcattggccc
    11341 atcattagaa gctcgcgatg tattggcggt gttgcaagga ttgcctgatg cacccaatga
    11401 tttgcgtgaa cgagcgttga cgcttgcggg tgctgcgtta gagtgttctt cgaaagtaca
    11461 accgggttta gggaaatcca ttgcgaagca attattggaa agtggacaag cctttaagaa
    11521 atttcaggcc atttgtgagg ctcagggtgg gatgagggaa ttgacaaagg cacgttttac
    11581 tcatcctgtt gtggctgcaa aggcaggaaa agtctccctc attgataatc gaaagcttgc
    11641 aaaaattgcc aagcttgctg gtgcgccaaa atcgaaatcg gcgggtattg acctccatgt
    11701 ccatgtcggc gaatccgttg aacaaggtga gcctttgttt acaattcatt cggagtcttc
    11761 aggggagctc cattacgcct gtgatgtttt acgtgacaaa caagacgtaa ttcttttagg
    11821 agaaagccct tgaagccctt attattttca ttatttgact catcagaaat cgccagacga
    11881 ttacaagaag ttctgcaagt ggacgtgggg gatgtgactt ttcatcgctt tccagacacg
    11941 gagtggtact taaaaatcaa ttcagaggtt aataatcgca gtcttatggt ggtggacagc
    12001 ttaaacgagc cggatgaaaa aatactccct ttgttgtttt ttgccaaaac ggctaaagag
    12061 ttaggggctc gaaaagttgg cttgattgct ccttatcttt cctatctacg tcaagataaa
    12121 cgctttcatc agggtgaggg gattacttca cgttattttg cacagatgtt atccagttca
    12181 ttcgattggt tgatgagcat tgatccgcat ttacaccgtt atcactcgtt agatgaaatc
    12241 tactcgatcc ccacttttgt tttgcatgcc acatcgatga ttgctacatg gattaaacat
    12301 catgttccta agccaattct aattggtcct gatatggaaa gtgaacagtg ggttgccgat
    12361 attgcagaaa aaggtcgttt tccctatgtg attttggaaa agattcggcg tggtgacaaa
    12421 gaagtgacta tttcagtacc cactattcct gaactcgaat cctcgacact cgtgcttgtc
    12481 gatgacatca tttccaccgc tagaaccatg gtagagacgg tgaagcagtt acaacaagca
    12541 gggggcaagt ccatcgtttg tattggtgtg catgcgctgt ttgcagaaga tgcttattct
    12601 ttattatctg agatgaaagg ggtgcaagtt atcacgtgta ataccattca tcatgtcacc
    12661 aatgcgattg atgtgagtga tttggctgtg gaagttctag tcaaaaacaa actggccgct
    12721 ttaaaaagta agctaaataa gttgattatt aacaaggagt aatccatgtt taaagccttt
    12781 agaatgattg cagtgcttat tgggttaatc ggaggactat tctttttgag ttcctgcgat
    12841 ggagcagtga agcatggctc ccaagaggtc gatggcggct atggaggaca cagcgcgggc
    12901 gggcatggtg gtcatggcgg cgctggccat taagcctgtt cgctgtctcg gtaaatcaag
    12961 tcaaagcgta ctcacacata acaggtaggg caaaccatga aacacgacca tcaccaaggg
    13021 catacccata ctgacaaaga acctgcttgc tttcacaaag agcatggcct ccacaagcgg
    13081 caagacacgg cttctaaaac cgatggtgcc gttatctata cgtgtcccat gcatccagaa
    13141 attcgccaat caaaccctgg catttgcccg atttgtggta tgactttaga accggaatcg
    13201 ataactactg aggagggagc gagccctgag tacctcgaca tgagatggcg attttgggta
    13261 gggttggttc ttacttttcc tattgtattg ttggaaatgg gagcccatgg atttgggcat
    13321 ttcatctcgg ccaatatctc aaactggatt caattgctat tagccacacc tgtggtattg
    13381 tggggtggtt ggcctttttt tgtgcgtggt tggcactctc taaaaacgcg ccaattaaac
    13441 atgtttactc taattgccat gggcattggg gtggcttggg cttatagtgt agtggctgtt
    13501 ctgtttcctg ggatatttcc ccttgccttt cgcagtcaat tcggcacagt ggctgtttat
    13561 tttgaggcgg cggcggtgat tacgactctg gtcttattag ggcaggtgct ggaattaaaa
    13621 gcacgagaac aaactggcag cgccatccgt gccttgctga aattagctcc tgaaagtgcc
    13681 catcgcatta aagaggatgg cagcgaagaa gaggtatctc ttgataaggt gcacgttggc
    13741 gataagcttc gtgttcgtcc tggtgaaaaa ataccggtgg atggggaagt gctggaagga
    13801 cgaagtttta tcgatgaatc catggtgacc ggcgagccta ttcctgttac aaaagaagcg
    13861 ggcgccaaag tgattggtgc aacaatcaat cagacaggca gttttatcat gaaagccatg
    13921 catgtgggca gtgataccat gctggcacgc attgtccaaa tggtcagtga ggcgcaacga
    13981 agccgtgcgc cgattcagcg attggcggat accgtctcag gttggtttgt gccggcggtg
    14041 attttggttg cggtgttgtc tttcattctg tgggcactgt tcggcccgca gccagccttt
    14101 agctatggat taatcgctgc cgtttccgta ctcatcattg cgtgtccttg cgcgttaggc
    14161 ttagcaacac cgatgtccat catggtcgga gtgggaaaag gcgctcagag tggcgttttg
    14221 attaaaaatg ctgaaagctt ggagcgcatg gaaaaggtca atacgttagt ggttgataaa
    14281 acaggtaccc taacggaggg gcgtcctaaa ctgacgcgta ttgtcactga tgatgagttt
    14341 gatgaagagg ccattttgac gtttgcggcg agcatcgaac atcaaagtga gcatccgctc
    14401 gccactgcca ttgtcatggc agcaaaagaa aaacaattgt cattggggac ggttgaagat
    14461 tttgaagcac ctactggaaa gggagtgata ggtaaagtaa atggccatcg tattgccgtt
    14521 ggtaatgcac gattaatgca agagtatggc agtgataagc ttgccctttt tgaaaaagcc
    14581 gatgaacttc gcagcaaagg cgcatcagtc atgttcatgg cagtggatgg taaaacggtt
    14641 gctcttttgg tggtggaaga ccccattaaa tcgactactc ctgaaacaat acgtgagttg
    14701 cagcaaagcg gcatcgaaat ggttatgctc accggagata gtaagaaaac agcagaagcg
    14761 gtggcaggta cccttggtat taaaaaagta gtggctgaaa tcatgcctga agataaaagc
    14821 cgcatcgtga gtgaattaag agacaaaggt ttagtggttg ctatggcggg tgatggggtc
    14881 aatgatgccc cggccttggc aaaggcggac attggtattg ccatgggaac gggtactgat
    14941 gtggccatcg aaagcgctgg tattaccttg ttacatgggg atttgcgtgg catcgttaaa
    15001 gcgcgtcgtt tgtcagaaat gaccatgagc aacattcgtc aaaatctttt ctttgcattt
    15061 atttataatg tactgggtgt gcccttagct gcgggggttt tatacccact aactggctta
    15121 ctcttaagtc cgattattgc agcagcagcg atggcactgt cttctgtttc ggtcattgtt
    15181 aatgcgctga gactacgacg gataaggcta tgaacaaaag atgaaaaaaa cggttgttaa
    15241 tagaaaaaaa acaaaagaga tctatactaa taggaagcag tcggttgttc ataaggcaaa
    15301 atcatggtgt tcccagctat tatgattgtg gttggtattg tctttctaat cggtggtgcc
    15361 tatttaatta aaagcgtact gcaacaaacg agccattatc gttggcagtg gctcgtttat
    15421 cttgttttta ttggcgcatt tattctaagt tattcggcca gtatttttta tggtctcttg
    15481 gttgatcccc attgtcttaa ttatttttat ggcagtgtgt ttttattagg cgctacctat
    15541 gtttatttgt cttgtcgtct tatgactaag accattcaaa agatggaagt aatggatgat
    15601 ttaaaaaatc gctatgaact attaaggcat catgtcaaat acgattcgct gactggttgc
    15661 cataacagag agactctttt tgaaatgttg aacacacggt ttcagcaaat taagagcagt
    15721 gatggatggg ttattgtcct ttttattgat atggataaat ttaaggcggt taacgatcgt
    15781 tatggtcatg atgtgggaga caacacgttg gcagcatttg gacaactcct aaggcagcga
    15841 ttgcgaaagg atgatattgt ggctcgttat ggtggtgatg agttcatcgc tgtgatggaa
    15901 cacatctcct tggatgaagc gaaaaagatt gcagaaaatc ttattacagt agctaagcaa
    15961 tccttcaaaa aaggtatttt atcgaagctt aatctaggct gctccattgg catcactcag
    16021 atgaatcctc actcggcttc agtgaatagc gtgattaaag aagcggattt ggcgtgttat
    16081 gcggcaaagc aaagaaaaat caatggttcc gtttgtgtct ataatccgat gcttgcaaca
    16141 aggaacgata cgattgattt gggtattcat caaattgccg aataaaaaat gaaaacacac
    16201 aaaacagtcg tcttactgca gtgcattaag cacgctatta gcgtgctttt ctgttttttt
    16261 gttctacctc gtcaattaat ataccactct tgtttaatgg cattgggtgg gagatactag
    16321 agaataaaat aataaaaaga agggattaat tcatgattca ttttcagatg aaacgcacca
    16381 tcctattgat ggtttctgta ctccttgtgg gctgttctta taatgccaag ttaactcctg
    16441 agggagaaaa agtgaccctt ctccaatcag gtaagccttc caaagcctgt gcttttttag
    16501 gggaagtcag agcctccgat cgtaatggca tgagccaatc ctatcaatcc catgatcatt
    16561 tgattaagga tgaattaaat atcttaagaa atgaagcagc tctattaggt gctaacaggg
    16621 ttttagtgac tcaacatcag caaacttatg aaggcaatcc caaaaatgat ttcgtagcgg
    16681 accattggat ggtaggtcga gcataccgat gtaagacgaa ttaaaagtta ttttgaatct
    16741 tattatttat ggaaattaat tctatgaagt ggttttttgg ttgggtgatg cttctattac
    16801 tgtgttcctc tctggctaca gcaggcaacg tattaaccct tcaagaaata actcaaattg
    16861 ctctagcgaa caacaaggat ttaaaagcag cccgttatac tgtttctatc gccaaggcgc
    16921 gtttaatcca agcaggtctt tggcctaatc caagtcttaa tctttccaat aacgacgatc
    16981 gcttatttaa tgacgaaggg gaatattcac gcagtgctgg ttttagccaa gcatttccta
    17041 tttcaggacg tattgcaaaa caaaagacag tggcgcgctt ggatgtgtta aaagccatgg
    17101 cagaaattag ggaagcagag cgtcaattaa gtagcaaagt agcgaatgcg tattatgctg
    17161 ctgtgattac cgagcgtcgc atgcaacagg tgaattactt attacggata aaccacgagc
    17221 ttgttgatgt gattcataac cgttatcacg cggctgaaat ttcaaagctg gatgacaatg
    17281 cagcacgcat tgagtatctg aggattggac aagaaaaaca tcttttgcat agttttagta
    17341 tcagtcaata tgccctactg aatcaattga tggggcggaa agccaattct cctttatctc
    17401 ttaagaggga tgtcatcaca ggtaaaaaac ttcctgagtt agccttctta aaaacattag
    17461 cccttaaaaa tcgtcctgat cgtcaggcca tcgctttatc gattcatcgt gccaatgccg
    17521 acaggcgttt ggcaaaagcc gagcgttttg cggattggac ggtagggctt ggtgttcaac
    17581 aagataaaat tgtggtggaa gagggaccac cccaacccgc tgatcgcacc ttgggggtca
    17641 cgctttctgt gcctcttcct cttttaaatc gtaatcaggg gcgaatttta gaagccagtc
    17701 acacaggaac tcaagcgatg atggcattac gtgccttgga tttagttatt gaaactgaaa
    17761 tagccagtaa ctatgcccaa ctgaaagcac ttcaaatgag tcttagggaa acgcaaactg
    17821 tttctcttaa attagcagtt gagaatgtca aactggcacg tgattcttat gagagcggac
    17881 aaatttcttt tttgaatgta ttgcaagtgc aaaaacaaca aaatgatttg caaacaacat
    17941 atctcaatac tttagaaaag tatttgcaag tgtatgtggc tttatgtacg gctattggtc
    18001 caggtaaaac caatgggttt tgctcttatt tagcgtatca aaggaatggg gatagcaaat
    18061 gagatggtgg cagcaaatga gaaggatggc attaattagt ctgtttctta gtctactctt
    18121 tcctattcaa gggtgttttg cccatggaga agaaattgaa gtgagcgagg gaggagccaa
    18181 aggccccgtt catttaacac cagcacaaac taaaatgctt gacatcaagg tggtggaagc
    18241 aaccaatcgc cccatggcgc aaatgcttgg tttaaatggc caaattcaat tgttacctga
    18301 tgcccaagcc gatgtcagta ttcggattag tgggagcgtg accgccattg atgtgaattt
    18361 aggggatagc gtcaaggcag ggcaacgttt ggccacagta cagtcaaggt tagtaggtaa
    18421 tcctcctcca agtgtcgccg tcacagcccc cattgcgggt attattgatg cgagaaatat
    18481 caatttaggt caagcggttg agccgaacac ggttcttttt cacatcagta atcgcgataa
    18541 attattagtc gtagcccaag tgtatgaaga agatttaggt aaggttaagg tagggcaaga
    18601 agtgactatt catgccttaa gttacccaaa gaaggttttt gcaggacaag taactttaat
    18661 tgaacccaat cttgactcct taactcgtac cgtgaatgtg cgcatcacct tggataatga
    18721 agagaaactc ctaaagcccg ggatgtttgt acgcgccaac gtcattttag ctcgtaatga
    18781 ggcagcactt gccgtaccta acgcggctct tttacaggcg gataatcagc aatttgtttt
    18841 tgtgcaaaat ggggatactt acgagcgagt ggatgttcaa gtcggtgctg tggatgatga
    18901 cttctcagaa attaccgatg gcttggttcc tggcgacctc gttgtcactc aaggcaatcg
    18961 tgagctttat acattatggc ttagtggtgg tagtaaattt caatccggca gtcatgagga
    19021 gccccaataa atgtttactc gcctcattgc ctggtcttta cataatcgcc ctttgatttt
    19081 ggcgttaacg attattcttt gtttactggg tggttatacc ctaaaacgaa tgccggtgga
    19141 tgtttttcct gaatttgcac caccgcaagt ggtagttcaa acccaagcgc ctggcatggc
    19201 aacccaagat gtggaaaccc tggtgactta ccctttggaa agcgctatta atggaacacc
    19261 aggggtagct agtgttcgct caaaaacctc cgttggatta tcgacgatta ccgtggtatt
    19321 tgatgataaa acggacatct accgcaatcg ccaactggtt aatgaacgta ttcaacaagt
    19381 agtaaaccgc cttcctccgg gggtttcatc accggtgatg ctgcccgtca cctcagcggt
    19441 cgggtggttg cttaaatatg ctttggtgag tggcagcgca agcccagaaa cactgcgaac
    19501 catttctgac tggactattc gtccgcgcct cttagcttta ggcggggtgg cttctgtagt
    19561 ttccttaggc ggggaagtca agcaatatca agtgcggctt attccttcgc gcatgctggc
    19621 ctatggcatc acggttgaag aagtgcgcca agcgttgaca acaggaaatc aaaatgtgcc
    19681 tggtgctttt gtccatcaag ccggtactga attcgtagtg agcaccattg ggcggattaa
    19741 aacgcttaaa gatattaaaa aaacactgat tgtagtccgt aatggggttc ctattaccat
    19801 tgataacgtg gctgcagtcg ctttcggtgg tgaaattaaa cggggtgatg cgtcttataa
    19861 tgcagaacat gcggtgattg gtactgtttc taaaatttat ggcgccgaca cattaaccac
    19921 gacacataaa gtggaacaag ctttagcgga tatcaaaaaa tcgttgccag cagggattga
    19981 attaatcacc gaggtgtttc gtcaagccaa ttttattgaa tcggctattc ataacttaac
    20041 gaaagccctg attgaaggag caattatcgt cattgcagta ttgtttcttt ttttaatgaa
    20101 ttggcgagct tcttttatta cgtttttgtc catgccggtt tcttttattg tcggcatcct
    20161 ggtgcttcat tattttggtt ttggtatcaa ttcgatgacc ttgggtggga tggcgattgc
    20221 gattggggaa gtggttgacg atggcatcat caccgttgaa aacgtggtcc atcgcttaag
    20281 gcttaatcgc atgagcgata aaccgttacc cgcgattgaa attgtttttg atgcggtttt
    20341 ggaaattcga agttccgtag tttatgcgac cattattatt agcctcgtgt ttttgcccat
    20401 tttctttcta tcaggaatcc ctgagcggat ttttagtcct ttagccattg cttatatcgc
    20461 atcagtctta ggttctcttg ttgtctctat tactatggtg ccggcccttt gttatctcct
    20521 tttagtgcga aaaagtgaaa aacagcaaga gaataaagtg gtgatgcatg ctttatctca
    20581 agaggaacgt tatcatttga cggaagaaga agcagccaaa caatctcaag cggaaactcg
    20641 ttttgtgatt tggctaaaaa aacatttttt aatggggtta aattggtcat tggaccattg
    20701 taaaacggtc gtgactatcg cgattggcgc atttgttttt gctttagctt tacttccttt
    20761 ttttgggact tccttcttac ctgagtttca tgagggcaat tttattattg tgatgagtac
    20821 gttgccagga acttcgctgg atgaatccat gcgcttaggc aatcaagtca gtaaaacctt
    20881 gttgaagtat ccgcaagtga tttccattgc tcaacgggca ggccgcagtg aattggacga
    20941 agatgcccta ccacccaaca tcagtgagtt cgatgtgcgc ctggattttg gcaaagacaa
    21001 gagcatatct ccggatgaat tattgcaacg cattcgagcg gatttggcaa acatcccagg
    21061 agcgctgttt aatgtaggtc aatttattgc ccatcgtatg gatgaagtgc tgtcgggggt
    21121 cagagcacaa gtcgcggtaa aactctttgg tgataatctt gcgacattaa acgaactggg
    21181 tcaatcagtc gaagccctat taaaaaccgt accaggaata gtggatgtta acaaagagca
    21241 acaaattaat gtaccccagt tggtcattac cattgaccga gaaaaagccg cgcgttatgg
    21301 cattaatgtg gggcaaattt ctgaggatgt tcaagttctt ttgaatggcg taaccgtgtc
    21361 gagtattctc gaagggcagc gaacgtttga tttgtatttg cgcatgagtg aaacaggacg
    21421 agatagtgtt aaagccattc aaaacatgct gattgacgct catggaacgg agaatcagaa
    21481 aactaaaatt cctttgcgtg cggtagctga tattcgccta gaaccccagc cttttgcaat
    21541 taatcgggaa aatgtgcagc gtgttttggt gattgctttt aatgtgcaag agagggattt
    21601 aggtagtgtg attcaagaag tgcaacaacg ggttaaggaa aaggtgacct tgcccagcgg
    21661 ttatttcatc caatacggcg gtcagtttga aagtcagcag caagcctctc gcgtgattat
    21721 tctttttggt gcacttgctg tttttatcat gttaatcctg cttcataagg ctttcggaac
    21781 atttcgtgag gcactgcttg tgatgttcaa tttgcctttg gctttgattg gtggggtcat
    21841 ttctttgttt cttgccggtg ctaacatgag tgtggcggct atgattggtt ttatcacctt
    21901 atttggaatc gcggcacgta atgggattat tttagtgagc cactacaatc aattgcgtgt
    21961 ccagggacaa ccacgtgagc aagtggtcat tcaaggaacc ctcgatcgct tagtgcccgt
    22021 actcatgacc gccgcaacag cagcgcttgg cttaattcct ctgttatggg gttcgcctgc
    22081 aggaaaggaa ctggagcgtc ctttagctca ggtgcttttg ggaggattat ttacttctac
    22141 tgtactcaat atgtttgtag tacctacggt gtataatgct attgaagtat ggcgagaaca
    22201 gcggatgcaa ttcaataaaa cggaacaagg agaaacaaca tgaagcacgt tactttgaaa
    22261 caaaaaagtc atttttggtt agggcttatc accagtgttt tgttggtttg tgggtattct
    22321 caagcaatgg ctgattctac tgaagagtca acacaatcca ttcccaaaac gattccagcc
    22381 atttgggagg ctgtagataa gcatgcggca tccattaatc aagtgcttag tagtgatgat
    22441 ttaacgacaa tacaccagca tgcatttgcc attcgtgatt tagtcaacgc attacctgca
    22501 ttaagcaaag atttgtcaga ggagcagaaa aaaacactgc agcaaaatct aagctatgtc
    22561 agtcaattgg ccactcgtct tgataaatca ggcgatgcca atgataaaaa agggacacaa
    22621 gtcaactggc aaaaattaca aaaagtgttg gcgcagctgc gttctttgta tccttcggat
    22681 gcccctaatt aattaaggca tcggttgtaa tatatcctgt gattcgcagt ttattactga
    22741 ttcacatttg tcctatatag ccaaacaggt gcaaatgcgt tttgcccaga gggatattgc
    22801 agttgctaaa atgagatcag acttgtcaag tatccgttcc ccattactga ggagtggtta
    22861 cttgactaaa tatattgatg taaaataacg tgcgcggcat atttgtttcg tctcttctgc
    22921 aaagatagct aaaaaatagg cttatgcaaa acaataaatt aggataagca tacatgactc
    22981 aggtgatgaa agagaacaat agagttaacg caagtttaac agcgctttcc gtgatgagca
    23041 gttatattca atctataaat aagccattaa taccttctga attttatatc gaactatgtt
    23101 tagcccaaca catcatttca tcagtggttg atttcgaatc ttatggctgc tcttatggag
    23161 atgaaaacag tctaaaaaaa ctgttacatt atttacaaca taattttttt aataaaacag
    23221 ttactcctgg ttatcactcc atgatattag ctagaaaact aatgctgaaa cacaaggtag
    23281 aaatgggtat tttatctaac ggtgtaagac aagttatttt tctaggtggt ggctatgatg
    23341 tacgagcgtt tatctcatca ataaaatttc caaacgttca gttttatgaa ttggataggg
    23401 gacaaactcg tcaaataaaa ctgaaagcac tcttgaatat tccaaaggaa attggaatcc
    23461 gacaaacacc agttgatata agaattaacc atacccacat ttttaatgaa aatttctttt
    23521 gtgtagactg tgatttttta gaagataatt tagaagaagt tctgtgtgct aatggattta
    23581 agaaaggaag caaaacatta gtaatcgcag aaggtcttac gatgtattta aatgaaaagg
    23641 gagtaatgca gttattagca gggttggcta atcttcttga ggagcatgat gaattcatat
    23701 tgagttttat tcctaaaata attaattctt tttcagaaga catgccaatt gaatctagag
    23761 aactgcattg tttttcttta ccgcctgacg aggttagtcg atttgtaaca gcttatggtt
    23821 ttgaggtgat tcataaaaat ttatttaaag atcttctgat gctcatgggg gatataaaca
    23881 atgcgaaaaa acatgaaaac aacctaattt taaaacaaga gcattattat acattgagaa
    23941 aatgcactca tttacttaaa gcaagcagca agacgattca tgagattcct gattatcaat
    24001 tttcaattcc attcaattgc tgtgcataag tgacaatatt ggtaggcagt gtttgcaaaa
    24061 tggcattagg ccatatcaac ttcttcctct agtagttcgc caaaccattt ttttacggtt
    24121 tcttctccta gatggtcata aatatagaat aaatatcgac tgaaatcgac ttcaaattca
    24181 ttgttaggta ttggctcgtt gtctgaagcg ggtgtatgat gtacatactt tccaaaaaat
    24241 tgatcacaaa aagccatata atcctttgta aaaagaagga aagtatgcca catgtcatca
    24301 atatccaaca tgtcagggta tattccacag gagaacttta attcgtcatt ttctggaaaa
    24361 tgacataaat cttctttatg tttttgagaa atccataagt atttcaataa ctctggaaag
    24421 gcttcttcgg cttttaattt attattggga taatcttttt tatacctatt taatacttgt
    24481 gtgtttttat attctaataa tttatcaatt ggcgttgtca taacaatgac ctcttaataa
    24541 gataaatacc attcacctaa gtcatgaggt gaatggtatc tttttataat actggtatta
    24601 ccaacaacaa agattacgtc ctttagtatt tttcattttt tttgtaacaa ttgtccttat
    24661 gagatctgac ttattcattt tagctccttt taaatgacat gagatggaat gatttttaac
    24721 attccgacat tacaatatta tgctagctaa ttgaagtcaa gtacggtgct tttgtatagt
    24781 taacgagtcg aatggtgcga taaatggtgt taacaacctt gatttagggt taatagaaat
    24841 actaggacag gttgattttt gttgcgtagc ctgctttgaa gtaatagtga ggtaggggag
    24901 aatctcaggt tctctatctt atcactctag caggtactgt gatggagccg gtgtataagt
    24961 ctgatacttg tctggataga aatgttctaa aaatcatcat aaacttcttt tcgatgtttt
    25021 attgcaataa taacgacggt atttgtttct gcttcgatgc ggtaaacaat cctataatca
    25081 ctaactcgca aacgtctatg tcctttaagg ctatatcgca gtggcttacc gaaaccgata
    25141 ggatcagcca tcaaacgctc ttcgatggct ttttttatga gctttttggc tgaagttgac
    25201 aaactaggca tgtcgttttt aacaacatct tctatatatt caatttgata cagtttactt
    25261 ccaggcgtca tcatgcttca ctctttttac tgtagcatga tcacgaaatt cagcaatggc
    25321 agacaatact ttatcttcac gtctttccaa tgcctccatg ataagttctt tagccaagcc
    25381 tgaaatcgat ttatgttcta gctctgcaag gtaggcaagt aaccctgctg ttgattcctc
    25441 aaaagtaata tttattcttg gatttttagt aggcatgata ttgctcctaa attggtgcct
    25501 aatataagtg taacactttt gcatcacctg tcaaatagta atactaatag aaacatttga
    25561 ttgagttaca tatcattcta tataactcgg gataaatttt ttaatcgaga atctaccact
    25621 atttattatg gaaattgcta tcaaacttcc ttcaacgtta agttttttct tttattacct
    25681 aactcaaatt ttttattagt atttttttcg ttgccacaaa agataaacca ctggaataac
    25741 aatcagagtg agtatggtgg cactagccat cccgccaacc atcggtgcag caatacgtcg
    25801 catcacttca gagcccgtgc cgcttaatag cataatcggc aataaacctc caataatggc
    25861 agtcactgtc atgattttgg gccgtaggcg caggagtgct ccttcgacta cggcggtata
    25921 cacattatct ttggtaagag cacgctgctc ttgcttcgct tgccgctgtt gcttttccaa
    25981 ggcttgatta agaaatacca gcatgatgac ccctgtttca gcggcaacgc ccgccaaagc
    26041 aataaagcca actcctacgg caacggataa atgatatccc aataaataaa gtagccaaat
    26101 gccgccgatt aatgccaagg gcagggttgc taaaataatg atgacttcac ctaagcgacg
    26161 aaaattaaga tacaatagaa aggcaataat agctattgtt aaaggaacaa caagggataa
    26221 acgctctttg gcacgctcta ggtattcata ttgcccggac catgccaacg aataacctgc
    26281 gggtaatttt acctgttgcc taataatatt ctgagcttct ttcacataag aacctaaatc
    26341 gcgtcctgca atatccacat aaacccaacc attgagtctg gcgttctcac tgcgaatcat
    26401 atcaggtcct tcggtaatcg taacggtggc cacttcactt aaaggaatag tggctcctgt
    26461 tggagtgaca ataggtagtg aacgtaagtt ttccaaagaa tcgcgaatct cacgaggata
    26521 acgcaagtta accggataac gttcccgccc ttcaatcgtt tgtgtcacat tcattccacc
    26581 cacggctgtt tcaacaattt gctgaatgtc ttcaatattt aacccataac gtgccgcttt
    26641 gacgcggttg atatctactg tcacataacg gctactagct actcgttcag caaaaacaga
    26701 cgttgtgccc ggtactttct tcaatacagt ttcaatttgt tgaccaatag attgaatggt
    26761 ttttaaatca ggtcctgcaa ctttaatccc tactggcgtt ttaatcccag ttgctaacat
    26821 gtcaatgcga gttttaatgg gcatcaccca cgcgttggaa agccctggca acttgagacc
    26881 tgactccagt tcatcacgca atttattcat agtcattccg ggacgccatt catttttagg
    26941 tttgaattgg atggtggttt ctatcatcga taagggagcc gggtccgttg ctgtttcagc
    27001 acgcccaatc ttaccaaata ctgttttaac ctcagggaca gttgcaatca atttatcagt
    27061 ttgttgtaaa agttgctgtg ctttacctgc cgaaatgcct ggaaaagtgg ttggcatgta
    27121 gagtaagtcc ccctcatcca gttctggcat aaattcacca ccaagataag agacaggcca
    27181 aaatccaata agcatgatga aggctgtgat tgttaatgtt gttttgggtg ttttcaaact
    27241 cgcttgtaga atcggtcgat aaacagccat taaggcacga ttgattggat tttcttgctc
    27301 cgcctttatt ttgccacgga taagataacc cattaacacg ggtactaagg taattgaaag
    27361 tgctgcagca gctgccatcg cataggtttt ggtatatgca agcggtgcaa acaaccgtcc
    27421 ttcttgagct tgtaaggtaa aaaccggtaa gaaactcacg gtaatgataa gtaaagagaa
    27481 aaataaaggt ggccccactt caagagctgc ttgttgcatc attggccagc gattttctga
    27541 agtaatggta ttatgctcta tgtgcttgtg ggcattttca atcatgacaa tggccgcatc
    27601 caccatggcg ccaatggcaa tagcaatacc ccctaaggac ataatattgg cattaatccc
    27661 ttgcacatgc atgattatca atgcgagcaa aatagcaata ggtaagctaa tcacaatgat
    27721 taatgaagag cgaaaatgga ataaaaaaac cacacaaatt agagaaacaa caataaattc
    27781 ttcaattaac ttatccctta aagtattaat cgctcgttgg attaaacttg agcgatcata
    27841 ggtagtaatg atttccacgc ctttaggaag acttgcttgc aattgattga gttttgctct
    27901 gacactggca atggtttgca tggcattttg gcctgagcgc atgacaataa tgccccccac
    27961 tacttcccct tcgccattta attcggtcat accgcgtcgt atttgtggac ctagttgtat
    28021 atgagcgatt tcttttagca aaatgggtat gccattttta cctaaaccta ctggaatttg
    28081 ctcaatgtcc tgaatggatt gcaaatagcc tttggctctt atcatgtatt ctgcttcgcc
    28141 catttcaatg gtggcgcctc ctgcttcacg attacctcgc tgcaccgctt gcttgacctg
    28201 gtcgagagtc aaaccgtagg cgcgtaatcg gttgggatct aaagtaattt gatattgctt
    28261 caccatgcca cctgcggtag ctacttcagc aacaccaggt actttttgta attcaaattt
    28321 gagaacccag ttttgtaaac tggtgagttg cgacaaatca tgttggcctg tgcgatcaac
    28381 aagcgcgtat tcatagaccc atccaacacc tgttgcatca ggccctaaag ctacttttgc
    28441 cccaggtggt aattgattgg caatttggct taagtattcc agaacccggg aacgtgccca
    28501 atacagatca acgccatctt taaaaatgat atacacataa gatactccaa aacctgactc
    28561 acctcttact gtaacagcac cgggtactgc taacatggcg gtggttaagg gataagtcac
    28621 ctgatcttca accacttgtg gtgcttgtcc gggaaattct gttttaataa tcacttggac
    28681 atccgataaa tcgggaatgg catcaacaac ggtgttattg atggtgtaga ggcctcctac
    28741 tatcatcatt acagtgacga gtatcactag aaaacggttg ttaattgacc aacgaatgat
    28801 gcttgcaatc atggtttttg tcctttgggg ttcgtggatt tcatccctga ggatttggct
    28861 ttttcatttt cttcaggtgt ttccaagcgc tccaaacccg cttttaaatt cgactcggaa
    28921 tcaagcagaa attgtccgga aacaacgacg ttttctccta ccttaaggcc cgaaagaatt
    28981 tcgactcgat cacccgattc catacctgct ttgacttggc gaacttgaaa acgtcccttg
    29041 cctaaggcca ctatcactcg atcaccttgt ggacttcgaa tcaaagcctc aagaggaata
    29101 ctcagtacat tggatttcgg attgaccagg atagaaatgc tggcatacat attaggtttt
    29161 aattgattat cgggattatc aaaacgaaat cttactttga cggtgcgagt catgggctct
    29221 aatcgagggt aaatgtattc taccctacct ttccattgtt tttgtgggaa cgctgatatt
    29281 cgagcttcgg cttggtcacc tatcttgacc caattggctt gttcttcaaa aatttgggca
    29341 atcatccaaa cgttggcaag atcaactaaa ctcattattt ccacatccgg tgtaactcgc
    29401 ataccttccc gaatattaag agcggcaaca atgccatctt gtggagcata aacagctact
    29461 aattgattcg attgatgatt ttttttgatg tcttcaattt gttgttcaga gatgtgtaag
    29521 gcttgtaatc gtttataaga cgctgtaatt aaggattgat tattgccttc taaggcgatt
    29581 agatattctt cttgggcagt tactaattgg ggtgagtaat attgcaacag caattggcct
    29641 tttttaacag aatcacctac tgccttgacc accagatttt tcacccagcc atcagcataa
    29701 gtatgaatat gactgatttg attttcatta ggctctacaa accccactgt gtctatatgt
    29761 cttgctaatg ttgtttgtat cactggaacg gtacgtacac caagattatt gactacagat
    29821 ggtgagattt gaactgttgc tttttcacct ttttcttggt tgtcgtctgg atagaccgga
    29881 acaagttcca tgcccatgcg cgattttccg ggtcctgggt aatgaatcat tggctccatg
    29941 ggatcaatcc aatagagtgg ttttttgttg gttgcctctt gtgaagcttt tccagtaatg
    30001 atgttagttg tccaacgccc caggaacact cccaaaataa tggagattac gattatgagt
    30061 actaatttct tgttcatagg gcaactcctt ctaaatacag tagtgttgca cgggatttca
    30121 ggcgctcaac ttgtatctgt acttgttcca gttgaatggt taattggctg ctgtaggcgc
    30181 gtagcacggt ggttaattca gtggttgcac tttgataagc caataaagcc gctttggcat
    30241 tttgttttgc ttcaggaagc aattgctttt catagatgac ctcacgttct gaaagacgct
    30301 gccatgtcgc atattgcgca ctgagtgcct gaagcaaatc tcgataatgt gtttgtcttt
    30361 caaagtggct cgcattgagt cggttgtaac tggcatttaa ttgcttatct tgtcgattag
    30421 cagtaaaaac aggcagatcc attgtaactt gagcggtcaa catatccgag cgaggcatcc
    30481 tgtctggcat gactccttgc ctgaagccat aactcacacc aagtaaccaa cctggtttat
    30541 attgttcttt ggcatacgcg acctcatagc gggaggcttt cacattggag gcatcggctt
    30601 gtaacaaagg atgctttggt aatcgggctt ttaactgatt caatggtggt ggccgagacc
    30661 acgcaggcat tgaaagagct aatgggcgat tgacaggatt aatcccaatc catcgaccta
    30721 gttgtgcgcg taaaatatca agttgctgct gaatttgaat ggcctgatca ttgagtcgtg
    30781 ataattccaa ttccacttga atcacatcgg attgattaat tttaccgtta ctgtattgag
    30841 attgcgtgac cttcagtaaa tccttatata acaaccgatt ggcgtgaaga acctgcaagg
    30901 ctttcgtcca ataatacaga tctaaccaag tttctcgtac gttacgtaac aaggtgatga
    30961 cttgatcaaa ggctttttta agctctactt tcgctaacgc ttgtgtttgt tgggatttca
    31021 tctttaaaga gtgaccccgt ggaaattgct gttgcaaacc gacctctacc atggtcatca
    31081 tgtcttgggt aaaactgaag gtatcggtag ggacattaat cgtacctgcc agtaattgtg
    31141 ggtcggataa ttggccaacg gcgacggctt gctgttgtaa cgctcgactg tttgcttgta
    31201 aacgttttaa ttcaggggag gtggcaagcg ctaaatgttc tgcttcggtt aacgtcaggg
    31261 gtgtattggc aattgcaaag gctagccctc cccaacatag gcaaatcatg gtcatgattc
    31321 cttgaagaaa acaggttgtt ttcatacgat agcctttaaa aaattaatgg aactaaagtt
    31381 tggttcatcc aatgtccatt tcgcatactt aagaatgaga gacatcacag tgcttatcga
    31441 ttgcgcttgt ttgactaatg aatcggataa tctaacctat tgatttatag gatgctcttc
    31501 taccatttta tataaatatt aaatagtata ttgtcgtaaa tccttactta tcacaatttt
    31561 ttattgagtt ttttaattta ttttgtcgag aaagggcaac ccgtacaata aaaaatcagc
    31621 ggcaaacccc ttgtgttagc tgtgagagaa ttgggtttat ttatattgca aataaaaaag
    31681 caacaagcaa catttttggt tctcattttc attggataaa attggatttt gtcctatgtt
    31741 gagtgcaaac gggccaggta aaatgtttat aaattaattt ggttttaaat ccaaagtaat
    31801 gacatcgtct gctttcccac ttttggggat gatagtcact gtgccttttg taaaagagcc
    31861 aacctggtag ctttcttctt ctagggtcac ggggtttcct ttttgatccc gtgcggagga
    31921 taaatccgca acttgtgcta actcttcaag actaatcgag gagccgcgat acaggtgttt
    31981 cgtttggttt gtagtgtcag tggcttcagt ggttccatga cccaaggtca caaaggatat
    32041 ggcgagcatg gttaataaat acaatagaaa attgattttt ttcatgagat tcatcctaat
    32101 caaatgaggt attaccataa tagattatct acggcgcaac aacaaacggt tacgactgga
    32161 ttatttgtta tttaatctcc cttaatcact tccttgagca gtcgttgact ttaataggtc
    32221 ttgtccatat cagtcatcca atggagagaa aatttttgta aaaatcttat ccctagtcca
    32281 caatagataa agaggtgggt tattcaggat ggtggtgatg catgggcaag gaaaataccg
    32341 attcgcctta tcaaatgggg catgattttt tttcttatca actggattgt tggcagcgtt
    32401 ctattttgtt ttggaatact ttgcgtgaac gcgccaataa tatgatggag cacgaacaac
    32461 aagggttgcc accactgctt aatttcaaat atgaatttgt tttggatggg aaatcattgg
    32521 agcctaaaac gaattatgca ttggttaaaa ttcttgaagt cggtgatgtg tgctttgaag
    32581 aatgttttga tcctcatgct catccagtta ttattgtgga tcccagatca ggccatgggc
    32641 cagggattgg tggtttcaaa cgggactctg aagtaggcat cgcgttacat agtggtcatc
    32701 ctgtgtattt tgttattttt tatcctaatc cggtgtctca tcaaacgctc gccgatgtcc
    32761 tgatgacttt gcgccatttt gttgaaaaag tccaagcctg gcatgaaggc aaagccccca
    32821 ttctttatgg caattgtcaa gccggttgga tgctggcttt attggtctct gattgcgtgg
    32881 gatcggttgg cttgacggtc atgaatggct caccagtttc ttattggtcc aacagcgaag
    32941 aagaagccaa tcccatgcag ttattgggtg gactcttggg tggttcctgg agtgcccgct
    33001 tcctatcgga tttaaaagaa ggaatctttg atggggcctg gttggtctct aattttgagt
    33061 tactcaatcc caccacggcc atttgggata agtattatgg cttatttgat gaaattgatg
    33121 gggagcgtga gcgatttttg gagtttgaac gttggtggaa tggtttttat caattcagtc
    33181 aagaagagat aatggccaca gtgaataatc tttttattgg caatcaacta gagcgtggtg
    33241 aaatgcggat tcataaagga tgtgtttacg atttgaaacg cattcaaagc cctcttgttc
    33301 tctttgcttc ccaaggcgat caaattaccc caccgaggca ggctttacat tggataagaa
    33361 ccatttatcc tacgacacaa gccttgaaga aggccaaaca acgagtggtt tatctgcttc
    33421 atccttccgt tgggcattta ggaatctttg tgtcggctaa ggtcgttcga ttgcaccatc
    33481 gtgctatttt agagcatagt gatgccattg agcaattgca gccagggctt tatgaaatga
    33541 taatcgttaa cccaacgggc gatcccgatt gcagtaaaga acaatatcat gtgcgttttg
    33601 aagcgcgaga acttacagaa ctttgcacaa cgagctccac gcaaccgttt gacaaggttc
    33661 gtcaaacatc cgaagccaat gactcaattt atcaaaaagt tatccaacct tctgtgcaga
    33721 gcttaagtaa ccctttttta tcctggtggc ttgagaaaac gcatcccatg cgcctaagtc
    33781 gctatgtgtt ttctgaaaaa gtgaatccac tgatgaaagc aatcgaacta ttaagcccac
    33841 ccattcaagc caatcgccgc atggctacac agagcaattg ctttaaaaaa atagagcatg
    33901 gtgttgcgca gatgatgagg gattcgttgg aatcctcccg aatcatgcgt aatgaggtga
    33961 tgaccaattg gtttgaagcc ctttataagg atcccgatta acaggcgagg acgttaattc
    34021 atagagcgta tggcatcaaa ctcctcgtca aggtccattt cattttctaa gtccctggct
    34081 tcatgaacct ctttctcatc aaaaagagat ttagtttctc taattttttc ttcgtggtca
    34141 ttctctgcaa aacaatcctt agcgttcata acaattcctt caagattatt gttcttgttc
    34201 ttgccaacga ctaaaaacaa cccggtttat tgttgctatt tttatctctg gcatttagca
    34261 taacggataa caattcaatt gacaaaaata ctaaataaga aataaaaagg gggtaactga
    34321 atagccattt ggctattttt ttataagaag aaacgaggga ataggatgtt ggttccccaa
    34381 actggaataa gaaacgcatc gaccatgata gaaattatcc ctaattggca ccccattttt
    34441 gtccatttta ccgtggcctt attttctgtt tcggtgattc tttatgcttt aatctatttc
    34501 gcctctaata ctcgatggaa aatcaacccg tttattgtcg aattggaaat cgtggcgcgt
    34561 tggtgtttat ggctggccgc attgagcacg attgccaccg tgtcggctgg attttatgcc
    34621 ttttatacgg taaaacatgg tgctatggtg cacgccgtaa aagtgattca ccgcaactgg
    34681 gcactaacta cctcgagtgc tattgtactt gtggctttct ggatggtttg gcgttatatt
    34741 aaacaccaaa aaccgacgct ggtcttctta atggcgttgc tctttgtcca ggtgttgctg
    34801 ctcacgacgg cttggtatgg agcggaatta gtctatcggc atgggtatgg tgttctgcct
    34861 gtcacagcag aaaagacagt atcaccgcat taatacgatt tttatggaag gatggaaaca
    34921 tgttacaaaa ggatttgcgt tgtctaataa ggaattattg ctttattttt tcctttttac
    34981 tgcttaattc ttctgctttg tttgcccagc atgagcaaca tggtgcctca acacaaccgt
    35041 taccttctcc caagactaca tcgtctcaat cgaaacctgc acaatacaaa atgaaacaac
    35101 ctatcgctaa aagccaacct gttagaacag tgaaacctct cactatgaca ggtggagcaa
    35161 agcgtacggt taatttggtg gttgcttata aaacggtgaa ttttgcaggc aaactaagac
    35221 gcgctattgc tgttaatggg caaattccag caccgacgtt gcattttaaa gaaggggatg
    35281 atgtcaccat caatgtgtat aaccatttgg atgagggaac gtccattcat tggcatggtc
    35341 ttttagtccc ttggcaaatg gatggggtgg acggggtgag tcaaaaacca attcctccgg
    35401 gaggcgtgtt ccattatcgc tttaagcttc accaaagggg aacctattgg taccacgccc
    35461 atgccaaggt tcaagaacaa gaagggttgt atggtacttt tattattgat ccacccaatc
    35521 ctccaagcta tcactacacc aaagattatg tggtggtctt atccgattgg agcaacactc
    35581 gcgccgagca agtgcttgcg aatttaaaaa aagacggcga ctattacggt cctcgatttc
    35641 ccctacaacc ctcgcttatg aagtttcttc atgattatcg taaagcttct cctgaagagc
    35701 gtaaaaaatt aatcgctgat tataaaatga tgcaacaaat gcgcatgagt atctatgatt
    35761 taagtgacgt ggcttatgat gcctatttat taaatggtca cccaaaatct catccttgga
    35821 ctgcccctgt gaaggtaggg gatagggtgc gactgcgctt cattggcgca ggagcaagca
    35881 ccatctatcg tgttaaaatt cctgatgcca aagtggagat ggtgcacatt caaggaaatg
    35941 atgtgagccc ttatcccatt gaggattttt ggattgcacc aggtgaaacc tatgatatcc
    36001 tggtgaccat tcaaaaaaac aagccttaca ttatttatgc cgagtcgatt gacacgctgg
    36061 gcaaagctta tggggcctta gtgacccatc ctaatcaagt cgtcaactat cagcacgtga
    36121 ctcctttccc tgaaccactt cctgtcacaa gagaaatgat ggccaacatg atgatgtcta
    36181 tgaatggcgg gggaatgggt ggaaatcaac aacacgcctt gatgaacaaa aaaacatcat
    36241 caacggcgat gaaaccctcc atgaccatgc cttcgcactc tcaagcaatg agttcgcaaa
    36301 gtgctaatca ttcgatgtcc tctaagacta tccaaaagaa aatggacaag gcatccaatc
    36361 attccgctca cacctcatca atgaagtcca aatcctcatc gacctcgatg aacaagagta
    36421 tggacatgcc aggtatgaat catggggcaa tgagtcagaa taagacgtct aactctgctc
    36481 aaatgaaatc tgactcctcg tcgacctcaa tgaacaagag catggccatg ccaggtatga
    36541 atcatggggc aatgagtcag agtaagacgt ctaactctgc tcaaataaaa tctgactctt
    36601 cgtcgacctc aatgaacaag agcatggcca tgccggacat gaatcatagc gcaatgggcc
    36661 agaataagac gtctaactct gctaatgaca cgtctatgaa taggaacatg aagtctgaca
    36721 ggcctatgga gcagggcatg tcgatgaatg gctccatgaa catgaagatg cccattgagc
    36781 cgaccatcat aggcgataaa atagagccac ctgattcggc aaaagccacc accttaggaa
    36841 ccaaatacca agagttaaaa ggggcagtga aaaccaataa tccgaataaa ccagtcgatg
    36901 ggattattaa aatggaattg tttggctaca tggaccgtta tatctggttt attaatggtc
    36961 taccggaata taaggccaaa ccgattttga ttgagccagg aaagcgttat cggattatat
    37021 ttaccaataa ttcgatgatg cgtcacccca tgcatattca cggccattgg tttattttac
    37081 gcaatggtca tggggcttac gatccgttat tgcataccat tgaagtccct ccaggagcga
    37141 ctgccgttgc cgattttgat actgaagcca gcggtcaatg gtttttccat tgccaccatc
    37201 ttctgcacat gaccgcgggc atggcgcgcg tatttcaata caccaccatc attgagattg
    37261 ccaacggcac tcgaaagcca gacaattacg cctatcagca agcctacatc aatcggccgg
    37321 tcgtacgaga agacgaagtc atgccactgg atgcttctct catcaaacac cctgcaggac
    37381 atcatcaagg cttctatcgt tcaagctata tcgagctggg cgaagaccct tttcataatg
    37441 ctcaggaaat gacgtttaga ggcctctatg gcccagatta caataagctc cagttgtata
    37501 ctgaggacgc tgaaatttat aaaggaaccg ttgaaaacgc agacatcgat gtgttctatt
    37561 ggcatttgat tagccaattt tgggcggtta aaggaggagt caattatttt tatcgcccgg
    37621 gaggccctta ttggcaacct ggtattggta ttgaagggtt aatgccttgg tatattgata
    37681 ccaacattag aacgtattat cgtgatggca gtgtcaagtt tgacattcag ttagcacggg
    37741 ctaaccagct gaccaataac ttcttcttcc tcacaggaat tcgcagcatt ttggccactc
    37801 ataccgtcgt caaaaacgag attggcaatg gcttaaacca aatgcgttat atcctgagac
    37861 cttattatcg tcttaagcca ggacttaata ttttttcaga gtatgagcat gatgtagagt
    37921 atggtgctct taagaggatt cttcgaagtc agggagagcc taccactcaa gatacaatca
    37981 cgttaggagt tgctgttctt ttttaaaaaa gccaaatata cattggaatt attttatgaa
    38041 ttcataggaa taaatagtca ccttcatgat attaatccat ccatgaggaa tatatcacct
    38101 atcaatgacc ttgttcattg gggtgttgag ctttctcttc ccaagaaaat ccttcctcat
    38161 gtctttagga tggggacaat ttatctgaca atactgggtt aaattatcct cagttttgta
    38221 atttaaccca gggtcctgga ttttttaatg attgatctgc aggtcatcat tttcaaataa
    38281 tggagtttgc ttaatttcta aatttctttg ttgtaattgg tcaactttca catggggaca
    38341 gtaataggca ttagctatta cggaatggat attaatagtt gttaagtttt ttgatcccct
    38401 tacatatctc ttatgagctt cacttggtgt atgggttaca agaacgataa catttgctcc
    38461 taacttcgca ccttcatttt ttaaaaaatt attatcatct ttttctagat cttttaaaga
    38521 ggtgcggata tccacaactt catgtacatt tgggttttta ataatcccta agaaacggca
    38581 atttcctgta ggataggaaa aatataagtt aacatttttc gcatctgcat ttacttgacg
    38641 taatccacca cagcccgaat tcaaaagaag aatcataaag ctaattatta aataaacaat
    38701 tttcatatct ataattcctt ttatttagcg tgcttttaac atacccatat aagactatta
    38761 cttactttga ttatgaataa aagtattaat tgtaaaaata taaaattaat gtcgctttca
    38821 attgagtaaa ataaatttcg ccattttgta tagtgatggt taaaatgcgt acctgatttg
    38881 cgagctaaag tcccttgtca cttgccatga tgttttcggc tacttctttc tcttgttttt
    38941 gataggtggt aataatctgc ttaaaataat taatccgctg ttactaatca ttggtcgttg
    39001 ttgatggggc acaagaggcc aacagaagca ttttgctgaa taaggccatt accccaataa
    39061 cggctttaaa aaatctctgt tttggcatat atccatgcta cttgcaatga aataaagtga
    39121 ttgtgaaatc aatagaaaaa caatccatct catcttataa atctttctta ataatatagc
    39181 accgtttaga aacaagtagt gtcagttata actacttatt gaaagaaaaa tcagctgtct
    39241 ttaggcattt taattaatac gcctaatcga atcattcgtc tctattaatt ttaccggtat
    39301 tggcatctac tcttaatttt actatcgtat cttctttgcc aagggctttc acttcgtact
    39361 ggctaccatg agattctatt ttgtaaatag aatgataacc agccccttca attttctggg
    39421 cggcttctaa tagggatatt ggatattttt gagtgttttg atttttgatg agatcgcccg
    39481 tttgtggatt cattcgtaat attatctgat ttccttgttc atcaagggct tctacttcat
    39541 ataatccgtc atcaaactca acttcttgta tagtatggta acccttactc agcaagtgtt
    39601 gcaggatttg cgacatgggc aatgaccgag ttggaatgtc atcagcgata gcaatttgtg
    39661 tcatacagga taaaataaat ccactgcaga ccgatggaat aatccgtttt ttcatagttg
    39721 ctcctccttg attaaaatga gcgcaatgat gctcgatgtg ctttgatgac gcaagtatct
    39781 catgatttaa gttcttccga gaccattttt tagtcgagta tatcttagcg ataccagtaa
    39841 aaaaaccatt atactcaata actcgtatca gtaatctttc catttgatgc ctcaggattt
    39901 ggctaaaagc gttttaaatg ccacaatcaa cagactgtcg cttactattc aataatcaaa
    39961 cttaataaaa aggaagaaaa tacatgaaac acttcacttt agtaaaaaaa ttaatggaag
    40021 ttcttttagt ttgtttatcc tttttattgt tttcctgcag cgcttccgtt tctaaagagg
    40081 actcaaaaag taacacgccg gttaagaagc gatttggtgg ctatcatcct catgtccatg
    40141 ggcgtggaac tcgttagaaa ggacctaatt aacactaatt agtatagaca attagtgcat
    40201 gggtatcgta tgaaagtatt tgttatacaa ggacaagtaa attccatttc tttcaatttg
    40261 ttttagggct tgattcattt tgttaattag cgccatcgaa ttttttttag ccatgatacc
    40321 ataacctatt ccaatcggaa tggtgtcccc aactccttta aataaccggt tgttatttgc
    40381 tatccaataa tcggcagaaa aatcatccat aagcagaaca tccacttcat attgatttaa
    40441 ggcggttatt aatttatctg tcgaagggta ttctttaatc gagttggatt tgaatttctg
    40501 agtaattaat tgtttaaaga gagagtccct gataatacct gttttcttat gggtaagctt
    40561 tttaaggctc ttgagcgaat tgtttttggt ggtgacaaaa cgtgcttcac tggctaaata
    40621 gggaagacta aaaagaaagg cttgttgtcg ttcgtcggta atagtaattg ctgcaatggc
    40681 gaggtcaatt ttacctggca tttaagtccg taaacagcgc gttaaacagc atgggcttga
    40741 aattgacgct ggatgttgat ttgtttgcac aattccgtca tgagttcgat atcaaatcca
    40801 aaaaaatgat ttttatctgc gcgtgagtcg tttgctacaa tcccaatggt taatgattga
    40861 gcccttagtt cagcggtaac gagtatttat aaaataaaga ataatcgtat caccatggga
    40921 taaaatgacg agggaccata aaacgcagtg tatctcaatt tgagaaaaat ttcagtgcat
    40981 tagcatcaga ttgtcttgat tttagcaata gggtgtatca agacacgaaa tcaaggtaca
    41041 atgaaacaat aaggtttgac atggattttc ttaaaaggta aggaatgcgt tatttttcac
    41101 gtctcgtcat tttcatgatg atgtacttcg ttgtttgcga agtactggcg gctaccccct
    41161 ttacttttaa acaggctttg gacacggctt atcgaaataa tcctgaattg caagccgaaa
    41221 tggacaaagc aagggccatg cggggttact ttattcaaag tggcctttat cccaatccgc
    41281 aactgacttt aacggcggag aatttcggtg gctctggcgc ctactccagt tatgaatctg
    41341 cggaaaccac gttttcagtc actcaaccga ttcctttagg ccatcgactt cagtatttga
    41401 aaaaagcaac ctatgcagac tatctggcgt ctcttgccag catcagagtt cagcaaacag
    41461 cgctgtatat ggctgtgggt gtggcttatg tggatgcgct atacgccggt caatggcatc
    41521 aagtgaccaa aaaactgact cgactgaatc aggatattgt ggcggctatt gagcgccggg
    41581 ttaaggcagg tgctggggca gagctggatt tgcgattggc gcaagtacga ttgggcgagg
    41641 ccaaaatcca ggaaaataaa gcagcacggg acgctttgtt gcagcgagcc aagcttgtgc
    41701 gcttgttagg tgatgggtta agagtggata ggcctttggt ggataaaggg ttaccaggtg
    41761 tttcagtcaa ttggactgaa ttagtaaaaa aattaccgca aagccctcag ctactgcaaa
    41821 tgcaactgca attgcaagcc aagcgcaaga cgattaccgc cgttaaaaaa tccgtttggc
    41881 cggatttaaa cattcaattg ggaggtcgtc atttttctga tgacggcagt aatgcggccg
    41941 ttatgtcagc ttttgcagaa gtgccagtat ataatcgaaa tcaaggcaaa atcatgacag
    42001 cagaagcgca atacacccag acggcccatg aatttcaagg aacgcgtctt gatgtgcgtc
    42061 aaagtgtcta tgcggccttt ttacaagccc agcaaaacca ctatgaagcc aatttggtta
    42121 cggattcctt attgcctttg gcacgtaaag cgataaaact ggctcaagag ggctatcaaa
    42181 tgggacgtta cacgtacatt gaattatcaa ccgcactcaa ttctttgtat gaagaagagc
    42241 ggcattacca gcaggctcat gccgattatc ataaagcctt aattcagatg acaggacttt
    42301 taggactgga taattctaag aaggagagca aatgacattc ctaaagccat ttaagccatc
    42361 cattctgctt cttattcttt ggtttgttat gggggttttt tcctgggcag gggagggctc
    42421 tcatgctatg gaagaaggca accatcaaga aatcatggaa aaaggccctc aaggtggtcg
    42481 cttattcaag gaaggtgatg tagcccttga gcttttgatt tttgaacggg gtatgcctcc
    42541 tcattttcgt ggctttgttt atgagaaagg tgaactgatt ataccaacca aggcccgttt
    42601 agttgtttat ctcacccgat ttaatggtaa aaaagagacc attaccttca ttcccgttga
    42661 gaatttttta caaagtaatg aagtgattgg agaaccccac tcctttgatg tcacgattca
    42721 attggagcta aagggcaaac gattgtcctg gcattattcc acctatgaag gacgaatcaa
    42781 aatagcaccg gctattttaa aagcggctga tattcaaact tcggttgctg aaagccaaac
    42841 gattaaaaca caattaaaag tggtcggaaa gattgccccc aatcgcgata cacttgctcc
    42901 gatttaccct cgttattctg ggattattaa agcgttgact aaaaatttag gcgatgaggt
    42961 cactaagggc gaagtgttgg tcaccattga aagtaatgaa agcctgcaaa attacaatat
    43021 taccgcaccc ataacgggga ccattgtaca aaagtatgct accaatgggg agcaggcgca
    43081 aaacacgaaa cccatttatg aagtggctaa tttaactact gtgtgggctg actttacctt
    43141 ataccgcaaa gaaactcctc gggtaaaaca aggaatggag gttgttgtga caggagatga
    43201 gggaaaacca aagtccacca gtgtcatttc ttacattgcg cctttgggaa ttgaagacag
    43261 ccaaaccact ttagcacgtg ctgtactttc taatgaagag cgtctctggt tgccaggcat
    43321 gtatgtgaat ggcgcgatta ccattaaaga aaaaacagtt ccggttgccg ttcttctttc
    43381 tgcaatacag cgtttgaatg ataaggacat gatttttgtg cggcaaggtg attattttga
    43441 agcaacaccc gtcattttag gcgaaaagga tgaacaatgg gtggaagtgg tgtcaggtct
    43501 tgatgttggt cagccttatg tcagcaaaaa cagcttcttt atgaaggcag aacttggcaa
    43561 ggaaggcgca agccacgagc actaaggatg gatgatgctt gagaaaatta ttcgtttttc
    43621 cctaaaacat cgttggtttg tcttattatt tacggcagtg attgccgctt tgggtgtata
    43681 caattttcag cgcttgccta ttgatgcggt gcctgatatc accaacgtgc aagtgcaaat
    43741 taatacccag gcttctggct actcaccttt tgaggtggag cagcgcatca cctttcctat
    43801 cgagttggcg atgagcggcc tacctagtct tgattatacg cgttctttat cgcgctatgg
    43861 cttatcgcaa gtcaccgtgg tctttaaaga cggcactaac atttattttg caagacagct
    43921 cattaatgag cgtttgcagg aagtaaagga taaattacct cctgaggccg aaacaacgct
    43981 ggggccgatt tcaacaggtc tcggcgagat ttttatgtat accgtgacga ataaaacgaa
    44041 tgtgcctgcc agtcaacgct acaatcctat ggagcttcgc accattcagg attggataat
    44101 taagccacaa ctgcgcaatg tcgaaggtgt tgcggaggtc aataccattg gcggctatga
    44161 aaaacaattc cacatcacac ctgatccaat gaaactcgtt cgatatcgtt tgagtttaaa
    44221 cgatatcgtg gaagcattag agcgcaataa tgccaatgtc ggcgcgggtt acattgaatc
    44281 caatggcgaa caacatttaa tccgtgttcc aggacaggtt aaaaacatcg cggacattga
    44341 aaatattgtc attgccagtt ttgaaggaac gccggttcgt attcgggatg tagcagaggt
    44401 ggcattaggt aaggaattgc gtacaggggc ggctactgag aacagtaagg aagtggtttt
    44461 gggtacggtg tttatgctga tgggggaaaa cagccgcact gtgtctgagc gggtggctgc
    44521 caaaatgaaa gaaattaata aaacactgcc tgaaggtgtt gaggcagtca ctgtgtataa
    44581 tcgaaccttg ctagtgaatg ccacgattaa tacggtaaag aataatctgc tcgaaggggc
    44641 gctattagtt tgtgtcattc tatttttgtt cttaggcaac gttcgtgccg cacttattac
    44701 agccatggta attcccctgt ccatgctgtt gaccattacc gggatggtca ctaataaaat
    44761 cagtgccaat ttgatgagtt tgggggccct tgattttggt ttgattgtcg atggcgcggt
    44821 cattatcgtt gaaaactgca ttaaacattt aggtgaacag cagcatgcat tatatcgtgt
    44881 gttaaatttg gaagagcgtt taaaggttat tgcgtatgcg accaccgaag tcattaggcc
    44941 cagtattttt ggagtattta ttattacggt ggtgtattta ccaattttaa cgctcaccgg
    45001 tgtggaagga aaaatgtttt tgcctatggc tgaaacagtg attattgcgc tgctcgcttc
    45061 catgctgttt gctttgacgt ttgttcccgc agccgtcgct atttttttac gaggtcgtgt
    45121 gcaagaaaaa gaaaactggt tagtgcatta cctctcacta ggttatgcca gagtgttgcg
    45181 tcgctgcttt cacgcccgtt ttcttgtaat tagtgccgcg gtagttttag tggtcgttag
    45241 tcttcttgtt gcttttcgtt taggtgggga atttattccg agccttgatg aaggggatat
    45301 tgccatgcat gcgatgcgaa ttcctggaac gagcttgact caagccatca ccatgcagca
    45361 tttagtggag gagcgggtaa gccaatttcc tgaagtaaag cgagtctttg caaaattagg
    45421 gacagcagaa gtagccaccg accccatgcc acctaatgtg gctgatacgt ttattatatt
    45481 aaaacctcgc aaggattggc ctaatcctaa aaaaaccaaa caagaattag tgcaggaaat
    45541 ggagcaagca gtaaaacaaa ttcctggtaa taattatgag tttacccagc cgattcaaat
    45601 gcgttttaat gaattgattt caggagtacg cagtgatgtg gccgtcaagg tattcggtga
    45661 taatatggat accttactgc aagtggcaga ggccattagt gctcaattaa aaaaagtacc
    45721 cggggctgct gatgtaaaag tagagcaagt cagtggcttg ccgcttctga ccgttgcgat
    45781 taatcgtgac gttttggcgc gctatgggct acaaattggt gccgtacaag atgctattgt
    45841 cattgcaacg ggtggcaaaa gaggtggcga gctctttgag ggcgataaac gctttgattt
    45901 ggtagtgcga ttgccggagg ccttgcgctc aaacccaaat gtgttgcgcc aagtctttat
    45961 ccctttacct ccttccaaag acaacgagcg gcattttatt cctctgagtg aagtggctac
    46021 tttagtgcgc agcgaaagcc ccaatcaaat cagtcgtgaa agtggcaaac gtcgtgttgt
    46081 agtcagtgcg aacgtacgaa atcgagattt aagttctttt gtgaatgaag caaaacaacg
    46141 tattgaacaa aacgtcaaaa ttcccactgg ctattgggtg acttggggtg ggcaatttga
    46201 gcagttgcaa tcggcctccc aacgcttgca aatcgtagtg cctatcactt tattaggcat
    46261 tttcctgttg ctgtttatga gttttggcag agtaagggat gctcttttgg tgtttactgg
    46321 tattcctttg gcattaactg gcggagtctt tgctttgtgg ttccgaggca ttcctttgtc
    46381 tatttcagcc ggtgttgggt ttattgcttt atcaggggtg gctgtgttaa atggtcttgt
    46441 catgattacc tttattaata aattacgtga gcagaaacaa gtgtatttga aagatgcggt
    46501 cttacaaggc tcgctggcga ggcttcgtcc tgttttaatg acggccttgg ttgcctcctt
    46561 aggctttgta ccgatggcct tagcgacagg cactggttct gaggtgcagc gccctttggc
    46621 gaccgtagtg attggtggaa tcatctcctc tacttttctc actttgttag tgttgccagg
    46681 tttgtattac gtctttcatg agcggcgtta ccgtaacaaa ttacctatta aaaaagccgg
    46741 cgaagacact tgattaacat ggagatgatc tctatatttt ctcgttggaa ttaaggcttc
    46801 ctgggcaaga catccagaaa gaggactatc acaaagacca ctaaggccac aaaagcaaga
    46861 taaaatccta tgtttgcaat gagtttcatg cggtgaatag agcttccttt ggcaagaact
    46921 atcaaaacca gtcctattga aaaaattatc cccgctgtaa taagaaaatt atggcttgcc
    46981 cagtcgagca tcgttgtttc tcacgatttg atggtattta cttaagtata gatgtcactg
    47041 ggttctttta tgcaagcagt aatgagtggc ttcagtgctt attgttcttt gacaattcct
    47101 gctaatgaac tatattaaca gttaaatact tatctaactg tttaattatt aattatgtta
    47161 actcaagacc agattatcaa gttaagtgac attttgcatt taatgggcga agtcaatcgc
    47221 cttaaacttc ttattgagtg cttaaaaggg ccgaaatcgg tttcggatct ggcagagcaa
    47281 ttgcagctct ctgtcccttt aaccagccat catttgagtc tgttgcgctc ggcacggctc
    47341 ttgatggcta atcgcgaagg caaacacatt tattacagta tttatgatgc gcatgtacgc
    47401 tgtattttag aggacatgct taaacatttt actgaagaga cggagaatta acatgcgttt
    47461 gctaaaacaa gaggattggg gtcgttattc ggtgagccat gcttcgatag gaatgaattg
    47521 tctgcgatgt cgaacttctc ttgctccggg ggcatatttt tacagtcaat gtgggaaatc
    47581 atgatgacag ctccgctcac cttaaccact ttttttgttg caggacttga ttgtcaggct
    47641 gaagaacaat taatcaggcg gcaattgcaa gctatccctg aagtggaaaa gatggatttt
    47701 aattttattg ctgaagaagt aacggttcat catcgattac ccaccattga acctatacaa
    47761 cagcagatag aagccttagg gatgagcgtg cgcgccaaac aaagcacccc tagcgacaag
    47821 gttcaggaaa aacttaaatt tgatgcctct tggcagattc ttgcattatc aggtctattg
    47881 gctctttgct ccgaaatagc agcttatctt ttggcaacag agcaatctct atgggccatt
    47941 attcctgcct tactggccat tattctcagt ggacctccta cttttaaaaa aggttggctt
    48001 gctttgcgca caaaagccat gaacattaac agtctaatgc tcattgccat aacaggggct
    48061 atctttatcg gtgattggcc tgaggcggct atggttactg ttttgtttgc attggcggaa
    48121 cgaatagagc gttattcctt agacaaagca cgtctggcta ttcgaagctt gatgcagatt
    48181 gttcctgatg tggctcaagt gaaaacagct aatggtcaat ggcaaacttt actggtcgag
    48241 gaagtgccac tgggtgctgt ttttagagtt aagcctggtg agcgtattcc attggatggt
    48301 gaggtggttt caggtcaaag tacagtcaac caagctccta ttaccgggga atccatgcca
    48361 gtgagtaagc agataggtga ttctgttttt gccggctctt taaatgagca tggggctttt
    48421 gaagcgaagg tgagcaaagc gtcaggcgat acgctgctcg ctaaaattgg taaagcgatt
    48481 gaacaggcgc aagctgagcg ggccccaacg caacgatttg ttgacgaatt tgcaagatac
    48541 tatacgccta ttatggtatt gattgctgtt cttgtcgctt taattcctcc tttcgtgttg
    48601 ggatattctt tttatgattg gatctataag gcgttgactt tattagttat tgcctgtcct
    48661 tgtgccctgg tcatttcaac accagtaaca gtggtgagtg gcttggctgc ctctgccaaa
    48721 catgggcttc tcatcaaagg aggaagctat ttagagacgg gttatcgctt acagttaatt
    48781 gcactggata aaactggtac cttaacagag ggcaaaccgg tggtaaccga ttttattact
    48841 tatgataaaa gtcaaaccaa tgaacacttg ttgttgcttg ctgcaagtct tgacagccac
    48901 tccgagcacc ccgtagctca tgccctagtg gcatattggc agcaagagca tcctgataaa
    48961 tcattgcttt cagtggaacg gttttcagcg cttcctgggc gaggcgtgaa aggtgccatc
    49021 cagggggaat tgtattatgt aggcaatcat caattagcag aagacaacaa agtctgttgt
    49081 ttagaggttg agcaagaatt gaaacgactg gaagaagaag gaaaaactac tgttatatta
    49141 agcaatagca ctacggcctt ggctattttt gcagtggcag atacgcttcg agccaacagt
    49201 cgagaggcga ttgaatcatt gcatcgacaa aggataaaaa cggcgatgtt aacaggtgat
    49261 aatgctctaa ccgctcaagc cattgccaaa caagtcggca ttgatgaaat ccaagctaat
    49321 atattaccaa cagaaaaaat gcaggctatc aataaactct tagaacaata tcaggctgtg
    49381 ggcatggtgg gagatggcat taatgatgca cctgctttag cgaaagccac tattagcttt
    49441 gcgatgggta agggaacaga taccgcttta gagacagcgg atgtggtatt aatgaatgat
    49501 aatttagcca tgcttcctct gtacattgac ttaagccgga agacagtgcg aattttatgg
    49561 caaaacatta gtctatcctt ggttattaaa acggtgtttt ttattttagc acttggagga
    49621 atggctaccc tttggatggc ggtttttgct gacatggggg ctagtttgat tgtggttatg
    49681 aatgggctac gattgttgaa ttcaaataaa acgaagaatc attaatacaa tcgctttgat
    49741 tgctttttca atatgttatg atgagataaa ccactgtttt tgtatttgtt gaaagggaga
    49801 ggtagatgaa taatactgga tttaagacaa tttccttttt gattggagta ttgttttagg
    49861 atgtagctta atgtcctgca ctaaaaccac cgcgacagaa acgcatcctg aaagcggtta
    49921 cgagggaggt tttggtggtg atggagggca taggcaccaa gagcattaaa acctctatag
    49981 tcagataagg atagattatt atgggagtta atactttgac tatagtattg ctattggcat
    50041 tgatggtatt atgccttggc cttcttgcct ttctttggaa aaaaaagaaa aatttgcgct
    50101 ttgttataac catattaatc ttccttatcc tagctgccat ttttttacat ggtgtttcct
    50161 tccaattgca ttaggtcttt tttattcatt ttaggaaatt atacgatgat aaagattagt
    50221 ataggggttc taaacgctaa ccgtcgaaat ttccgtgcaa taattaatca tcccaaaatt
    50281 ctttaaattt tacggaagca atttctgtgt accttacggt atgttgaata tttttatgac
    50341 ccaaataatg ttgaatactg cgagtatccc gaccgtcatt agctagtttg aagcctgttg
    50401 agtggcgaag catatgaggg tatgtcgcca agccaagttc tgctttttct cctgctcttg
    50461 cgataatttt tctaaaagta tctgcagtaa taggtgcttt acgctccgtc ataaaaacat
    50521 aatcggtttc tgggtaatcg cgttttaatt gacgcagagc ccgaatctct gggccaaaaa
    50581 gagggtgact ggctgggatt ccattttttt tgcgaatcac atgaagtaga ccttgtttta
    50641 aatctatttg agaccaacgt aatgagatca attcagatac gcgtagttca tgtcgatagg
    50701 caattaaaat cattgtagag tcacgtaagc catgccgccc caattgcttt gcagacgcaa
    50761 taagcttatc cacctcagct ggagtaaggt attctcgtgc tctgcgctca ctatttttta
    50821 cttttttagg cggtttcatg attcactttt atacactttc cctaaaagag ttattggtgt
    50881 cacttccagg catccaaaca acatgtttat ttataagaca attcacgatt taattttagc
    50941 aaactttccg ataaaggtat tctttaggga gagtttttta gtaacatatt gtctcttttt
    51001 taattaagat tttttatcat gcgtgcaagc aatgaaagat taaccatttt atctgaggca
    51061 gagcaagctg ctttgtatga gatccctgat tttgatgatg ttcagcgctc gaactatctc
    51121 aatctcaccc ctgaagaaca aatcttgatg cgtagccgtg ataatttatc aactaaagtc
    51181 cattgcgcta ttcaaatagg ttacttcaag gcgaaacatc ggttctttcc atttaagtgg
    51241 gacgaggtac aggaagatat tgatttcatt atgcaggaat attttcctga acaacaattt
    51301 cttccaaccc ctatcagcaa acaccaatat tatgcacaat gcactgcgat tacgacgcat
    51361 tttggttacc agtcttggtc aaaagagttt gaaccattat tgactgccca ggcacaacag
    51421 attcttcgcc gtgatgttag tccacaattt atcgtcatcg aattactagc ctttatgcag
    51481 aaaaagaaaa tcttgcgtcc tggatatacc actttgcaaa cgattgtgag caaagtctta
    51541 aatgctgaac gcaatcggct agaggtaatt cttagagaat ccctgacgat agatgataaa
    51601 tccgctttgc aaaaattgtt gctagaaaaa gaagcccaaa cgctttctgg attagcagat
    51661 ctcaagcagg ataccaagga ttttaaagca cgcatgatca gtgctgagcg cgaaaaatcg
    51721 atttcgataa aacccctgta tcagttagct aaatcgctct tgcctacact taatctttct
    51781 cagcaaaata ttcgctacta cgcaagtctt gttgattatt atgatgtcta tgaattacgg
    51841 aagaaattaa aatctgagca gacctatctt tatcttctgt gttatctctc tttacgatac
    51901 cttcagttga atgataattt aatagacgct tttttcctga gcctcaagca atttgaagca
    51961 gagcttaaag aaaaaacaaa agatgcctat acggactacg cggtaagcaa acaaattgat
    52021 tcttttgtta tgcgtcgatt tgcacggtta tttatcaacc aagagctaca tgatcaactc
    52081 agctttggcg atgtacgcaa aaaagcattt gaaaccatta tccctgaaga agagcttcgc
    52141 cataaaatac ccgaagaaaa tgaggaggag cttaagccta tcgattttca atggaaactg
    52201 gttgatactt tatttcatcg atgtcagttg cagttacgac cattaatgat ggccattgat
    52261 ttctcaagta ctgcagatga aagtccttgg cttaaggcaa ttacctggct taaggagcta
    52321 tttggtgcca acaaaacgat aaaccaatat ccagtcaccg caaaccccga aaaaaccagg
    52381 caaaaacgct tggaaaaata cttgtttgat gcgaatgcca agggtgagaa aaaattccat
    52441 gccgatcgct atgaattttg gatttatcgt caactgaaga aacggcttaa agcaggagaa
    52501 ttctatttgg cagacagtgt acagaatcgc tcgctttaac aagagcttaa ttcagccaat
    52561 gacaaagggg cgctagtgga acccttagat attccagcat tgagaaagcc tattaaggac
    52621 ttactagacg aacgttgtgc tgaattgcga tcagaatggt tgaaatttaa tactgatttt
    52681 actcaaggta agctcaagca tctgtaccat gatgaacaaa ctcaaacgct ccatctaaaa
    52741 aaatcgagtg atgacaaacg cgatgaagat gctcaacacc gtttctatga acaattgccc
    52801 ttgtgtgata ttacggatgt acttcgattt gttaatgaac ggagtcgtta ttcatctgct
    52861 tttaccctca tacaacctcg ctatgttaaa ttgcttgccg atgaaaacac tctgaatgca
    52921 gtgattatcg ctcaagcgtt gaataacggc aatcttaata tggcagaaat ttctgatatt
    52981 ccttatgcca gtttgttgga cgtttaccaa tcccgtgtgc gattgcaaac attaaaaaaa
    53041 gccaacgata tgattgggga tgatattgca caaatgccta tttttcctga ttactcgttg
    53101 gatatggcta ttctttatgg tggtgttgat ggccagaaat ttgaagtggc aagacctaca
    53161 ctgaaagcaa ggcgttcaaa aaaatatttt aaaaaaggga aaggcgttgt ggcttatacg
    53221 ctgctagtca accatatccc gctgcaaact gaactgattg gtgctcatga acatgaaagt
    53281 tatttcactt ttgacatttg gtataataac accagcagcg tcatacctga tgccattaca
    53341 ggtgatatgc atcttattaa taaagctaac tttgctgtga tggactggtt tggtggtaaa
    53401 ctgtgcctta gatttaccaa tttgcaagcc cagctcaagc atctttactg tgatgatgat
    53461 ctatcacaat atgcagattg gttaatcaag cccgtagaca aaattgatcg ccaacttatt
    53521 gaggatgaat ggcctaatct ggaaccgatt atcaaagcgt taggctgtaa agaaatcact
    53581 caaagcattc tgattaaaaa attatgcacg tacagcgcct caaacaaaac cagaaaggca
    53641 ttatttgaat acgataaatt gatccgtagc atctataccc tgaaatattt gcaaaacaga
    53701 aaattgcaac gagacataca tcgttctcaa aatcgggtag aatcctatca tcaactacgt
    53761 atggctattg caaccgccta tggtaaaaag caattatcag gcaggggaga tcgtgaaatt
    53821 gagatcagca accaatgtgc caggcattat tgctaatgcc attattcatt ataattcggc
    53881 aattctatcc aagctcaaac tcaaatatga agctgaggga gatcataagg cattagcact
    53941 gttgaagaaa atttcgcctg tagcttggca acatatccat tttcagggac actttatttt
    54001 ttctggtgat aaaatcattg atctcgatgc aataataaac aaagtttttt tgcatggagt
    54061 aaaaaagaaa aaagtggttg aatttagtcc ggaaatttcg acggttagcg tttagaaccc
    54121 cttcaacgtc tttaaagtcc acatattgcc gctccttgta aaagagctga ttaagctact
    54181 ctaaatccaa tacttatcta tttaagtata gacaagatag cactgaagtt cataaattga
    54241 tcaatttatg aaatgatacc tgactggcgc aatcctttag aaggatgaac tatactaata
    54301 caaattactg agtcaaagga gtgcacatca tgtcgcctgc atttgggcac cacccgaaat
    54361 caccaaaacc acaaaaagag aagggcttct ggagttctcc ttccggtcta atggccatca
    54421 tcattattgg gattattggc tattacttga ttgtagagca tggtgctcac atagcaagct
    54481 ttttaggcgc ttcaccattg gtgctgttag tcttactctg tccattgatg catcttttta
    54541 tgcatagagg acatgggggg catggagaga atcatcacca taaatcggat gacgaggaca
    54601 acgattcttc tgagaataag gactaatcga aaatggaaca tacttcttat gcctatggtt
    54661 tgtggttatt agtcattgtc aattcggcga ttttcatttt gtttgcatac agttttacca
    54721 ccacgtttaa aacaaggcga gattggcgcg cctttggtgc attttctgcg tttgttatcg
    54781 cttattttac cgaaatgtat ggttttcctt taaccattta tttgctgtca ggatggcttt
    54841 ctaaatatta tcctggaatg gatttatatg gtcatgacag tggccattta ctccatacct
    54901 tgcttggctt gaaaggtgat ccccattttg atgtctttca tatcttaagt tttgtcttca
    54961 ttatcggcgg tctttggatg attgcatcag cctggggtac tctttatcgt gctcaaaagg
    55021 cgcatcaact tgcaaccagt gggctgtacg ccaaaattcg tcatccgcaa tacgatgggt
    55081 ttattctggt gatgattggt tttttattgc aatggccaac cattctgacc ttaatcatgt
    55141 ttcctatatt agtttatatg tatgttcgtc tcgcgcgtag ggaagaaaaa gaggttttgg
    55201 cggagtttgg tgaggaatat aaacactatg ctgcagttac tcctgggttt atacctcgta
    55261 ttagtaaaca acaagacagc aaaggaggtg caccatgatg tttatgtttc acagtgccga
    55321 gatgctcggc cttatcgcaa tagcgcttgg aatcatgttg gtcgtctggg cattacgtaa
    55381 tgaaggaaaa agtattggat tagccaaaat atttggatgg ttgattgtca ttatcgggat
    55441 tctaggggag ctatgcagta cttattatgc gattaaatac tggtatgcag gctatttcca
    55501 gatgccaatg atgtcaggtg aagcattctt atttcatgtg gctcagatgt tgtctcttat
    55561 tgcaatagca ttgggtatta ttttgatagc atgggcgtca cataacgaag gacgtggtat
    55621 ttcgctagca aaagtctttg gttggttcat cgttctcttt gcgattttgg gcatgacttg
    55681 tagtgcttat tatgccactg tgtattggta taaaggatat attcaaacac ctgctggtat
    55741 gcctatgttg ttgcaacatc aaataccgat gcagaaacag tcacccaatc aatagtcctg
    55801 gtaagtgcca taacaaattg ttacggcacc tataaaattt tccgttgtag agaaagagtc
    55861 cctcaatgat gcttataaaa tccaactcct ttaatatcag tccacaacgt ggtttggtgg
    55921 caaaggaaac attgatcgac acgggcttca tgctcatgag ccactttttt tgaaatcata
    55981 ttaaaatgtt ccatataatg actgggtggc gcttgatggc attgagcaca atccattgaa
    56041 ctcggagatg ggtgcctaaa ttcagagagg ctccaatgag ccgtttcatg acaagcagaa
    56101 caatcgtcgc caaataactt aaagtgcttg tctttgtttt gatggcaaga ggcacagcgt
    56161 aaagtcagtt cttgaggtga taaatggggg ttgaccaata cggttggcct attggattca
    56221 ttctgcgcta aaaagagcgt cacctgatta ataatcaact gttcttcttc atcatttgca
    56281 tttgaaaagg cacgaagttg ctgaagtcct aacgaaacca aagcattgtg atccatctgc
    56341 gtaatacgtg ccgttctgcc ctggtgctct ctatgacatt caacacaact gcctatgttg
    56401 gcatgaaagg aggttggctg gcgttttaac agagattcgt tgttggcatg gcagacgata
    56461 cagttcgctg cctcaacccc tttcactgga gtgtgacacg cagtgcagtt gtgacttaaa
    56521 aacgagtggg ctttggaaag ttctccgggg cttgccattc tttgccactc cgccacgttc
    56581 gtcaaactgc tcacctcatc tccatgagaa gacagataat aggccataaa agacagtgcc
    56641 gcgattacgg taagggcagc gagggtagcc catctcagct tcattgaaac caccttaatc
    56701 caaaataaat tccagaccaa acgtgcaaac ccagcagaac atacaatacc agtgctgtaa
    56761 taatgtgaca ggttaaccat ttttgaaaca ggcgcttgaa gtgctcgtga aaacgtatgg
    56821 cgtattccag ctcagagatg gcatctgcta aacacaaagc gcgtacttct gccgacttga
    56881 ttaactgacc atccagtgcg gtgtcttcaa gcagccagga aagaaagcga gctgtcataa
    56941 gatgtaattt cccgatgttt tgcttggagc gtgcgctttc ttgtaattct tgagctacga
    57001 tttggtattg cttttgtaat cccaaaagga tagtcttttt ttcatcgatt tcctgagtca
    57061 taaagcccat gagataacgg ccaataaaac cactgagcgt gacaatgagc gtcatagcag
    57121 tcaaagcaat acctaaagcg ctattaaatt tatgccctgt atgaatcaag actaaaatcg
    57181 agccgataat acctgtgtaa atatgccacg acaacagggt attcatggac acatagtgtg
    57241 ttacccaatt tttcaaaaat gaaatacgtt taataaagag gtagagtagc ggaatgagca
    57301 tcaatatcga gccactcacg ccaaaaacgc caccccaaaa gctcccggca aaacgagaag
    57361 atgtatggac tgcaaagcct agccatgtga taagcattag aagtactaac cccaaaacca
    57421 tcattcgtcc ttgcgcattc attaagcatc cacctcaatg ttttgagtcg ctttggcctg
    57481 acaggctaag ataagcccct gttgtttgtc ttcctttgaa agcgcatctt cacaagccat
    57541 ggtcacttcc cctgacagca aggtcacttt acataacccg cattgcccgg ttcggcacgc
    57601 attatcaata gcaatcccat tggcttcagc gatttcaaga agggtacagt ccggcagaat
    57661 tggaaccatt ttttcagatt tccgaaaaga aatcatcgca cgggtttcgg ctttgatggt
    57721 gactaagtct tcttgagcta tttctggttt cttttctggt ccaaatgctt ctgttagaat
    57781 caaatcagcg ggcactttaa gctcttttaa gctaccaagg atggcttcca tcatcgcagg
    57841 aggaccgcaa acgtggatac gatgtgaggc aatatcaggg acaagatggc tgataatatt
    57901 cttggtaaat aggccttgta accccatcca aatggttccc tctgaacgca gcatcgacgc
    57961 atacacatgg agattgaggt atctttcttg aagttgctct aattcttcgc gaaagagaaa
    58021 ctcactggtc gtgcggcaac agtacagcaa ataaatgtca ttgtgccatc caatatcggt
    58081 gaggtagcgg atgatactca tcatcggggt aataccaacc ccgccacaaa tcaatacgat
    58141 gctctttgcc tcttcaccag taaaagtaaa tttaccatta ggccccatca cctccagtaa
    58201 atccccttcc ttaatttcgt catgcaaata acgggaaaac aagccttgtt cttcccgctt
    58261 gaccgtaatg gcgcaataat gcaattgggt tggggtggag gccatggtgt aactgcgtct
    58321 tatcgtttta ccattaatta aggcggtgag cgtaatgaac tggccaggat aataggtaaa
    58381 tggcaaagcc acctcatggg ttgatgccaa atgatatgtt ttgattcctg gtgcttcttg
    58441 aaagatacgg caaacacgaa gttgtccttc ccatttctct tccggttcgg tgagtgatct
    58501 catgaccgga aattgcggga ctggacattt atgagcgggg caggtaggag gcatagttgg
    58561 ggcagtttct gatggcactt tagaaacttg agaggattta atgggctcta ctgatgttaa
    58621 ggaaggggtt ggttcaggtg cttttgagaa ttggggtggt gtctgcgaag atacagccgc
    58681 attttgtttg gttaagcgat cgagcagagc agcagctctt ctcattttga aaaaatacat
    58741 ccaaatcatg accaaaaaaa acagaccaaa tagagcgata atcgttaaat gaaaaaaagg
    58801 cccacctaag acactctcgg cttgcggact acttggaaga agattcattt ctcgtttaaa
    58861 ccactgcaag gcgatgtcgc ttggattttt tccttcagac agtgcgcgaa gtgcggccag
    58921 tgcactctca tattgcgcaa tcccttcccg taatttctct aaagcttctt gcattttagc
    58981 gtaatcgtta ttgttggatg ccaacaaaag ctcgttaagt ccacctttca tgagcgccat
    59041 gccggatgtg atgcgttgat gtgccttttg ttgaattgca aggcgttttt cgggggataa
    59101 tgtgggtaaa ctcattaaag aggggtaaag ttccttagag ggagtcccta tatttttcat
    59161 cataccactc attccctcgc cttctttttc tccttgatga tgggcagcat ggtcttgtgc
    59221 tgtgagttga gcaaaggaaa cagaagccat cgccaacagg aaagaaatag atactgcttg
    59281 aacccataga aataggaaaa actttttaat gtattgaaac agcatccttt ccctccaaag
    59341 cgcactgaac aagctgagat tatttgtttt cctgcctctt attagtttat cccatattct
    59401 gtaacaaatt tccaataact taaaagggta tgattaggaa ttcatgcagt cattcattcc
    59461 atggattaga aactcaacca aatcagtacg ttatttgctc tattttgagg ttctgtctat
    59521 tctataatag aagctttttt aagagatgtc ctatgtggaa tcttaaaaaa gtgaagggac
    59581 ggcacgcttt gatactttac agggatcaat cggttaagtg ctaattctga catggtagtg
    59641 aacattatga tgccaggatg gaggaatttc caatggcgaa gcagtggatg gttttaatag
    59701 ggtgtgtggt cttgagttta ttgacgactg cttcgttagc acaatatcga aatggtgttt
    59761 tttcagtgga gtactcaaag gcctccccta taaaaaatat ccctcttaaa aaagccacat
    59821 taataatcaa aatctactat tatggatatc ccaaagggca tttctcagtg gtcacggatg
    59881 aaaaacaaca ttttatcatg ggttatgatg ataagtatca aatcgctctc gaactcatcg
    59941 ccatttcggg acaggaacaa tataaggcct tatgccgtgg agaatcgaaa ccagggcagt
    60001 tgaaattaat agtcgtctgt aacccgtata agaaaaagac cttataaaga ggcggtgtga
    60061 cttttgcctc atagtgctct atgtgaactc atgaccaata atcgcttggg cgctttgcaa
    60121 aatattctga atttcttccg taatttcttc ttggcgcatt aaattaagac gatggtttaa
    60181 tgcatttttt ttattttcta accgatctaa tgcctggttt aaatgaaaca gtctttgatg
    60241 attctccgca aaaaacgatt gataaaaaat cgcataacac atggccagta aataatgctc
    60301 caccaagtca gcgaaaaact ggtctttgga accattcaaa acaggtggca cagaaaaagg
    60361 aggcgcagga cttatatcca actctttaaa gggctgccag gtttttactt gaatttgatt
    60421 gtgctcttcc tcattgaaga gaacagtcca ttgccaagag tgccatgtct tatttctttg
    60481 caataaggct ctttctaacg tgtgtaacac gtttgaaatc acggctggga tttcttcaat
    60541 ggcattgggt ccatcgattg ttgccatgac acgagaatca ttagccattt ttaatgctag
    60601 tttatgccct acaagaacga gtgctggttc ccattcagaa tgttgctcgt tgtgcgcgtc
    60661 taactgatga agtacattat cattaaaaga accacaaaag ccgcgttctg agccaataag
    60721 aatagaaact aagggaggat tttcttgttg attcatgggg ggcatgggat agaaacttaa
    60781 aaaatcatgg ctcacctcac gaatggtttt aatgacgttg tcctgcatgg aaagacattg
    60841 agtgattttg cccagttcaa tgaaagaaag atttttcatg gccgtcataa tgtggccaat
    60901 ttcttctaaa gtatgaacat gttctttgag tttggttcgt ttcgtcatga taccatccgg
    60961 gtattaagtg atactcattg ctttggagtg gataatgagt ccgttctatg ccattagcca
    61021 ttcttttatc gcttttttcc attgttctcg tgggcttccc aaggataaag tgctttgttt
    61081 tatttcttcc tctatttttt tgagcatttt ggggatgtct tctaaattca attcgtcaaa
    61141 gaaaccctca ttgtaagcaa taagccatgc gagttgaaat tcaatgggta gtggtgaaaa
    61201 acgttcttgt tttagaattt ctcttaaaac tcgccctttt tgaatctgct tttgcatttt
    61261 ggcatccagt ttcgccccaa agcgggtgaa cagttccaaa tcaagaaatt gcaggtaatc
    61321 cagtttcatt cgaccggatt cttttttaat ttgcggatgc tgcgctttac caccgactcg
    61381 tgagaccgat ttggtgatat caatggcggg aagaaatcca gaagagaata aggattcatc
    61441 aaaaaagatt tgcccatcag tgatggagat gagattcgtt ggaatataag tggccatttc
    61501 accttctttg gtttcgataa taggcaacgc cgtcatacta ccgccgccca gagcagggga
    61561 aagacaggtg gagcgttcta ataaacggga atgcagataa aaaatgtccg caggaaatgc
    61621 ctcacgtcca ggaggtctac gcagtaaaag ggacagttca cgataactat tggcatgagc
    61681 actcaaatca tcatagatca ccagggcatc taagcccttt ttcatccagt gttcggcaat
    61741 ggcgcaccct gcaaaaggag ccagataaag taatcctggt aaggccgtcg cttgggctac
    61801 tacaacagtc gtatagtcca atgcattggc ttcttttaat aattgaatgg tagagcttac
    61861 cgtggaacgt ttttggccaa tcaacacata gacacaatac acttttttat ctttttgatt
    61921 catcacaatg tcaagggcca aagcactttt accaagccca ttatccccaa tcagtaactc
    61981 cctttgtcct tttccaatgg gaattagatt atcaagcatc ttatttcctg tgtacaatgg
    62041 tcgattcaca aagtcacggt gaagaatagg tggggacagc ctatccagta atccttgttc
    62101 ttcatggggc ggaatctcac cgccgtctaa tggatggcct agaggatcaa tcactcgccc
    62161 aagcagttta tcacctacag gaatactcaa tacgcgtttc agaggaaaaa taggggtgcc
    62221 tgcctttaat tttttggttt gtaccagcat cacagcgcca accagctctt cggtgaggtg
    62281 gaacaccatc gcgatacagc actcatcctc tgaaataaga atttcatcga tggcagcccc
    62341 tggtaagccc ttaatccaga taatcccatc tccaacagaa accacccgcc cttgttcgga
    62401 taccttgatt tgaaattgat agcgctcaag gcgttgtcgt tgcttctcaa gaaaagaagg
    62461 ggtgttagac caattcattt tttgtctcta taaagaattt taattcatca cgaagattgg
    62521 cttgcaagca catggggccc atttgaaggg ttaatcccgc gagaagcttt ggattttccg
    62581 taaaagagac tgtaattttt ttaggtgaca attgttcaat aacttgcaag agatcctgtt
    62641 tctgatgttc ttggatgggg taggccgttt gtattgaaac ggtttcttct tcaggcacag
    62701 tatggagcca ttgccattgt tctgtcggaa tatgatggag ctcctcaatg gtttttttga
    62761 tgattttctc ttcaagatgg gcatccgcaa aaggcattaa caattgttca gcaaatttcc
    62821 cagccaaaag aaatgcttcc ttggcattgt tttcaatgat ggctgcggct ttttgcattt
    62881 catgggaaaa aatctgctct ttttcttgat gcaattgttt ttcaaactgg agtctttctt
    62941 ctgatttcca tcgttccatc gcctcatgcc actcgttttg tagggtcgct ttctcttgtt
    63001 gccaatcggc aagacgatgt tcataggttg tttgcaattg cgttgcttgc ctgtgcagtg
    63061 tttgtgcagt ctctaattgt tcttgcactc ttttttttcg ttctaatatg gttttttgta
    63121 tgggcgcata aagaaagcgt ttcaaaatcc aaatgagaat aagaaaatta atgagttcta
    63181 atgaaaaagt ggtccaagag agttccattg gttttatccc tgttgcgtta caaaataaga
    63241 caataatgga tttctaaaga ggataatcaa aacaatcacc aaacaataaa tggctaacga
    63301 ttcgatcatg gccaaaccaa taaataaggt tctggtgatg gatttttcgg cttcaggttg
    63361 ccttgccaaa gcatctaagg cgtgactaat cgcgcgcccc atggctagag ccggccctat
    63421 agttcctatg gcaatggcta ttgctgcaat aacggttgac gctaaactaa accaacttat
    63481 gtcattcata acgattctcc ttggctttta agctcatggg cttgaatgcc cccagcaatg
    63541 taaattaagg ccagcatgcc aaaaatatac gcttggatga tggcttcaat aatatgtaaa
    63601 atgagtattg gaatagggac taaaaatccg gcaatcatga ggacaatcag ggcggttaat
    63661 tgcaagctca tgatattacc aaacaagcgg acggccaatg ctaaggttcg agagatttcg
    63721 ctgattaaat gaaaaggcaa taaaaaagga gttggcttaa tataatgttt caaatactct
    63781 cgccacccct cggcacgaat tccaaaccag tgtaccgata aaaaagtcat tatcgcaagg
    63841 gatgccgtca ccgataaatc cgccgttggt gaataaaatc ccggaataac accaattaaa
    63901 ttggaaacaa ggataaaaat ccacaaggtg gctacaaaag gaaatatcag ctcaacgtgg
    63961 tcaggcaata cttcttttat cgcatcgtac atggtgctca aaacaccctc ccaaatgagt
    64021 tggtacgtgc ttggctgtag gagagagcat ttgtaagtgg tactccatgc catgatgaat
    64081 aaggagatca taatgaacca cgtggttaag acactttgtg tgatgggtaa tccaccgatt
    64141 gaaaaggcaa aatgcgctaa gaatccttct tctcccacgg ttagtcctta attaaaagat
    64201 acacgttaat ggcgccaata atcaccccaa ggaggattaa attgatggtc caactcatgg
    64261 aatacccttt tattttctca tcgagccaaa ccccgagata ggctcctgca atgatgggta
    64321 acacaaaaac aaagcctaaa gtacccaggt aaactgtttg ggctaataac gttgattttc
    64381 cttttttagc tttattgatt ttgcggacgt ttcgtttcac ttgttgttcc agttccttgt
    64441 ttgaattttt aaattccata gctgttctgc cggtttaatt cccataaacg ctttaacatt
    64501 tcttcatcca aacgatgcac gctttcttta gtgcgcaata ggttgttttc ttcccaatgt
    64561 aattcttcct ccaggaccct ttgtatggct tggtattctt gatgtcgcac ataatgtctt
    64621 gtcgcaatat gcaattgatt gtcaatgaaa taaaccacgg cacctggtaa ggctaagtac
    64681 tccgtctctt gattctcatc acgaaaccag gccaagccga attttaagca cgtcatcatg
    64741 cggctatgat gagctaaaat cccaaattgc ccagaagcat cctcacccac aaaactgatg
    64801 accttatcga ttttttcata ttgtgtggca ctcttaagat ggatagtaaa aagctccatg
    64861 tccgtcgtta tctcccttta tccatggcac ccctcatgta acactcttct tctgaaaatt
    64921 catcgtaatc gcctcttaga aaggattcgc aatcagttaa tgtgtgttct aatgaaaccg
    64981 atttcccttc cattcctgtt tgaagcttgg tgacatgaaa gggttggcta agatagcgtt
    65041 gcagtttgcg tgctcttaac acaatagcac ggtctttagg agaaagctct tcaatcccca
    65101 tcatagaaat catgtcttct aattcttgat aacgttccag gtgttctcgt acggcttgtg
    65161 caatggaata atgtcgctcg cccaatagga ttttatccat aaattgactc ttggaagcta
    65221 agggatccac ggcgggataa atgcctttgc ctgcttgtgc gcgagagagt actatgatgg
    65281 aatccaaatg agtaataatc ccggtcaccg ccggatcgct catatcatca gccggaacat
    65341 aaaccgcttg taccgaagtc actgctcctt ttgcagtcga tgtcatgcgc tcttcaagtt
    65401 ccgctatctc agtcattaag gtgggttgat aaccgacact agccggcatt cggccaagta
    65461 accccgaaat ttcactgccg gcttgcacaa aacgataaat attatccaca agaaaaagaa
    65521 cctcatggcc caacgtatca cgcaaatatt ccgcataagt taatgccgat aaccctgtgc
    65581 gaaaacgcac tcccggtgat tcgtccattt gaccaaacac catgagggtt ttatccatta
    65641 cgcctgcgga tttcatctca tgccacaatt catgtccctc ccgaatacgc tcaccaacac
    65701 cagcaaagac ggaggttcct tgatgcaatt gaataatggc atgcatcaat tccatcagga
    65761 gtacggtttt tccaactcct gcaccaccaa acaatcctgt tttacatcct ctgacgaaag
    65821 ggcaaagtaa atcaatgact ttaattccag tctcaagaat ggtttcttga gtgctcgtca
    65881 tttcaagtgg cgcgaagttg gccagaacat cacgatattc atgggtttct aatggcgggg
    65941 ctccgtctaa cggctcacca aaaatattta aaaggcggcc caagcattct tttgaaacgg
    66001 gaattcgtaa ggaagtgcct tggtcataaa ctattaaacc tctttgcaat cctgaggcac
    66061 ggtggagcgt gatggctctc acatgatgct catcaaggtg ctgacacact tccaaaatgt
    66121 attcatcact gtccgtgtaa gttttaagag attgatgcaa aggaggtagt acatcacaat
    66181 gtatccggac aacagggcca ttgatttcga ttatcctgcc gatgggagtt ccatgagttt
    66241 gagcaacagc cgtgttttta tccatcattt tccaattgaa ttcatgaccc atgaattagg
    66301 tgtcgtccta ttctctctat ttttacatca ctttatggaa ttttaatata acagagtttg
    66361 aaaacagata gcaaaatgct aattaataac agctcgcctg aaagtatcta aaaaacaaac
    66421 actgtgtgag atctcattaa tcagcttcta cataggtcac tttgccacat gatacatcaa
    66481 gccacgcctt taaatagagt tcgacgaaat aggtaggtcc tgaatgggga caatcttaat
    66541 gagctactga cctataatag ggctcataga aatctcatag agccctggtg attggtttaa
    66601 gtgccctagc ataggcctct tatagaacat ttattggttt ttcatcatgg ggcatcccat
    66661 catttgtcta tccatcatca tctgcatcat ttgcatcatc atttgcatca ttttcgtacg
    66721 ctgctccatc gtttcgggtt gtttagctgc atcatcctgc atacttttca tcatcttcat
    66781 gcctttttgc atcgcatgca tgccttcttc catcatgggc atttgtgttt gcatcaacga
    66841 tttcttttct tccgttgttt ttgcattcat aagcttatca tgcatctcct gcatttgttt
    66901 tagtgcttct tgcatttgtt tcatttcgtc ttgcagttgg gttgactgct gattagagct
    66961 ttgcattccc atgctattcg tattattcat ttgagaacca gaagacatgt cttctgcaaa
    67021 gagtggtgtg gcaaccaaac tgcaagcagt caataaaatg aatacattcc gttttaacat
    67081 aatgaaatct ccttattatt ttttcaatga attgtttgga ctgaacatcc tactatagca
    67141 ctacatgaac aggggatgaa aagcaaaaaa catacctgct caggttagtg ccaacctatt
    67201 cctcccgtgt tgtgtccttc ataagtatga gaaacacggt gtgaattatt ggtcgttgtt
    67261 gatggggcgc aagagatcaa caaaagtatc gcgctcatta tcccaataac tgctttaata
    67321 agcctctgct ttggcatatc catgctcctt gcaatgaaat catgtgatgg ctgaatcaat
    67381 agaaaaacaa ctaaccttat tttataaatc ctttttataa tatagcaccg tttagaaata
    67441 cgcagtgcca gtcatgacta cttgttgaaa aaatcagttg tctttcggaa tagggaatct
    67501 atacctcgcc aagcttaata taagttcaaa ttattatttg aattcactgc caaggatgtg
    67561 aatagatgtt ggagttaagg aatacgataa gtagggtatt taattattgc gaagattcta
    67621 ccattctttt atgaagaaat tttaagatta ttcaaaagga aaa
//
LOCUS       DASLOE010000003        60662 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.003, whole genome shotgun sequence.
ACCESSION   DASLOE010000003 DASLOE010000000
VERSION     DASLOE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 60662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60662
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.003"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..629)
                     /locus_tag="U6309_000123"
     CDS             complement(<1..629)
                     /locus_tag="U6309_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0716 family Dot/Icm T4SS effector"
                     /protein_id="HEN5503594.1"
                     /translation="MFDDTNMSKNESGTAKPIIKSTLMFFNSKLTDLNDKRLQLNRGK
                     GLAKYLAKSQKNTRDLAPLDAALGVMSFSLYMLRFSANVGLLAHLILVESQEKTNHKI
                     RRDIYYSLLNDSLWCVVNLTQFFWLSYRNSISAGLQGMQLETLAQLIDILVMIIRYQQ
                     DKKEYDLKYNQAMGMERSRLAIEWQNKELNGLRSLLTSLSVVIAFGLFS"
     gene            760..1431
                     /locus_tag="U6309_000124"
     CDS             760..1431
                     /locus_tag="U6309_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HEN5503595.1"
                     /translation="MRLLIVEDHKRTADFICKGFNEYQFQADAVYDGAEAIYRISEIN
                     YDAVVLDVMLPIMDGWAVLEKIRELKPDLPVLMLSACDDVDSRVKGLEMGAYDYLVKP
                     FSFNELLARVKSLLRRKPKESPILLKINDLTLNLQKHTAIRGDKKLPLTAKEFMLLTF
                     MLKRSGEVLSRTVIAEQVWDINFDTNTNIIDVAIKRLREKVDNDHPTKLIHTVRGVGY
                     VLEIR"
     gene            1412..2509
                     /locus_tag="U6309_000125"
     CDS             1412..2509
                     /locus_tag="U6309_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN5503596.1"
                     /translation="MYWRFANSMSITNRLLLIFIGSTVLILSIITGLIYPPMKELLHH
                     SHLNNEHYNYLLTRICIKNFFVGLWFSTLIIILASYLLAKKSMRPIKIFTKELASINA
                     SSLEKRLPYTGNPKELEELALTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLRTA
                     TEITLTKPQTIETYQHVLQTHLEEYQSLTQLIDNLLFLTRCEHGQLQLNVRTLSANHL
                     VSSIIEYYYSIAQEKGIEIQMFGEAQIEVDEHLFKRVISNLIDNSLTYTNKDGRILII
                     IEKRTDNSIQIRIKDNGIGISKEHLPLLYHGFYRVDYSTNKENAGLGLGLAIAKSIME
                     HHKGQLAIDSKMGLGTTVILTLPGKSFLKIE"
     gene            complement(2651..4651)
                     /locus_tag="U6309_000126"
     CDS             complement(2651..4651)
                     /locus_tag="U6309_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEN5503597.1"
                     /translation="MSDTPFIAANKKVAELTFRVIILAIILTVLLAMSNAYLALKLGI
                     LTSASIPAAIISMGILRFFKNSTILENNAVQTAASAGEAVAGGIVYTIPALIIIGFWN
                     HFDYVTNFFIAVCGGILGVLFSIPLRRILVNDQTLKFPEGRAIAEVLKSSADKVGIKD
                     IFLGGLVGGLLELLQIGFKVIASSWNYWFVVKRSLFGLGAGFSATMIGAGYLVGHDMA
                     ISIFLGAVISWLIALPIASQFYPDFLNQYPAEQAATLLWNSEMRYLGIGAMLFAGIWT
                     FIKLAKPLTRSVKTSFRTYISKSKFREQYPRTEKDIPMPFILMGIGLMAGILFLFFQI
                     IFPLGQVGLDNDFSPTIVFIGVIYVLVVGFLFSVITAYFSGMVGVTASPGSSVVISGM
                     LFAAWLLVSAINHLLPLPLTSHQIQAAEAITIIIGAVVTGIAAIANDNTQDLKVGQLV
                     GATPWKQEVMLLLGVVISALVIPPVMQILFNVYGIAGVMPHAGMDVTQSLPAPTAALM
                     AAITEAVFRNSLPWNMMLAGSCIILLAIFLNRILNIKRFLNLSILGIAIGMYLPLSSS
                     VPLFIGGMIALFVQNRLGRLKVNPEESTNRKQKGTLIACGLVAGSAIMDVLLAIPFSI
                     FHSPDALQLVGSSWKQYGVYLGVFSTLLLAWWISHRVCRSKI"
     gene            complement(4651..5346)
                     /locus_tag="U6309_000127"
     CDS             complement(4651..5346)
                     /locus_tag="U6309_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HEN5503598.1"
                     /translation="MPIQMNLINKIGLMFGLLFYSFLASAITTEVIKKETADYLLDIK
                     YPQGFQSNGVNQAVKEFITNQQKSFMNELSEDADTPADAPGKTGLNITYAIPYQSTNA
                     LSVRFNISIYHRGAAHPSNAVKVLNFIKNQPVQLSDLFVPGSDYLQPIADLAKKEITA
                     KKISDENWIKEGTKPTQENYSIWHFTKKGIAIVFNTYQVAAYVYGEQTVDIPLSLISA
                     MVKPEISKAVWGN"
     gene            complement(5373..6584)
                     /locus_tag="U6309_000128"
     CDS             complement(5373..6584)
                     /locus_tag="U6309_000128"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027223834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEN5503599.1"
                     /translation="MFACDELLLNASTIDATGLQLSNQAIVIKKGRIEWCGSEDQLPA
                     HFQESAKSRKDCHGQLITPGLIDCHTHLVYAGHRAAEFRLKLQGVSYADIAKSGGGIL
                     STVQMTRDASEEELINQSLPRLLALKNEGVTTVEIKSGYGLDLQNELKMLKVARQLGE
                     MAGVRVKTTFLGAHAVGPEFKGNSQAYVDFLCNEMLPAAKNMDLVDTVDVFCESIAFS
                     IRQAEQIFQAAKDLNLPIKCHAEQLSNMGASSLAARYGALSCDHLEFLDENGALNMVK
                     ANTVAVLLPGAFYFLKEKQKPPVDLLRQVGVGMAIATDSNPGSSPTTSLLLMMSMACQ
                     FFSMSIPEVLSAVTYQASRALGMEKDIGSIEAGKIADLVLWSIKDSAALCYYFAYPLP
                     HQTMVAGEWVS"
     gene            complement(6584..7393)
                     /locus_tag="U6309_000129"
     CDS             complement(6584..7393)
                     /locus_tag="U6309_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN5503600.1"
                     /translation="MSGIISPIVFYIDEEIVYLITGGGSGIGRALAFALAKRNKQVFI
                     VGRREKLLQETASLSANIHYLCADVSTPQGRELIKASLSTTPQIDGLINNAGTLEPTL
                     LLKDISFAEWHQALNTNIDAALFLPQLLYDKLSGGRVLNVGSAAAYFPIKGWGAYCVS
                     KAALAMLTRCWQIECDSLAFASVMPGIIDTHMQAIARNGINLDPEQVNFYRRLKDHNR
                     LISPETVAEFFIWLLLDIDNETYVSKEWDIYDTTHHSAWLKPPHQVLHWEF"
     gene            7683..8642
                     /locus_tag="U6309_000130"
     CDS             7683..8642
                     /locus_tag="U6309_000130"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEN5503601.1"
                     /translation="MFDDLSNYRPANPALWQGRKDTANQERFFQKITFIDNQNELMTK
                     DKKTIFLGFASDAGIKRNLGRTGAKLGPDQIKTQLAKLPCHNNKHYVDLGNVVCENDE
                     LELSQSQFAQIVHFCHENGHQICAFGGGHEIAWAHYQGLSSLYPKLGVINFDAHFDLR
                     PYKKGELGNSGTPFSQIATYCEEKKMPFHYCCIGVQKFGNTPSLFEKAKKLNVSYLSA
                     EDLYEQSQAWQIAFLDDFILNLDHIYLTICLDVLAECYAPGVSAPQALGLSPWQIMPL
                     LKYLIQSGKVVSLDIAELSPPLDSELKTARLAALIIAELLDTN"
     gene            8655..9629
                     /locus_tag="U6309_000131"
     CDS             8655..9629
                     /locus_tag="U6309_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HEN5503602.1"
                     /translation="MKKTVLWGALFTLITTQANSEATQQAISTQNSASTGTNVVSEST
                     NQVPATNQNTQQNQLNQPVQSNGQQPTQTIQNNQQNQPSQTGEQQSNQNTQNNQQNQP
                     AQQQTTQQPAQQQVPSQQTAPVINCDYKISAETKAIEQSLVLTWSEKAIIQSFDFDPA
                     TVDAQLQKLQACFTDQGWLSFNSALQKSGNLDAIKSQKLHVSSQIDGQPQVTEAIENQ
                     WKIIIPLQVVYQNDKEKVTQLLNVNVTVGRKITGDLGINQMIAVPRTATNTSQTNSPS
                     NTNPADANTSTPQTPGDTTNNATQTNTQTQTGTPTNSPPSGSTTNTQN"
     gene            9686..10858
                     /locus_tag="U6309_000132"
     CDS             9686..10858
                     /locus_tag="U6309_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEN5503603.1"
                     /translation="MRLIFTGVLCLLTLNAQANVIQYFAGISYNNPADLFKVKNGILL
                     VGGTGSYADLQFKGSALNFNTFQYDSGVNHSRTYIVWPYGRVAKRLNDKTVVAVDLTE
                     PFNSNLDWGNDAFTRYAATQNYLTDVDLSPKISYAISKKLQIGGGINFNVLLKNEVNW
                     AFPTGQSTYANLINRSSSFGVGYNLGINYAVNDTNFLGITYYSRIRQNTSGTSYLGLA
                     ANPDFQFGFYMPATTVASYVHIFNPKWLINLQVFQSEWNANQKVRLYNTAAPPPFTNF
                     IFDMHFDASYAYLAAIRKQVSDKLGIALAGMIDDGPEEDGLRTIVFPSDTQYFLGLIG
                     DYRFTEHASMELILGHVYSNPSIQNKAKVNNVPVPFTTGRVTINANVLDLKVKIEG"
     gene            complement(10898..11323)
                     /locus_tag="U6309_000133"
     CDS             complement(10898..11323)
                     /locus_tag="U6309_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEN5503604.1"
                     /translation="MYNIMSFLCGLLFGAGLTISNMINPNKVLNFLDINGFWDPSLLI
                     VMAVAVTVTFLGYKLINKFDKPILCNQFYLPEKKNIEKKLILGSIIFGIGWGVAGYCP
                     GPSITALTTFNSDPVYFVIGLIAGSWTYYWLFKKRTIQK"
     gene            complement(11323..11760)
                     /locus_tag="U6309_000134"
     CDS             complement(11323..11760)
                     /locus_tag="U6309_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEN5503605.1"
                     /translation="MDYITSFTPYLSAIGGLLIGLSAVLFLWLNGRIAGISGMVHGLF
                     PPEKPFPVWRIAFLLGLAAAGLIYFISPVIQFPLRTHYPVFLLLIGGFLVGFGTRMGQ
                     GCTSGHGVCGLARFSKRSFIATLLFILSAMITVYLIRHVGGLY"
     gene            complement(11870..12481)
                     /locus_tag="U6309_000135"
     CDS             complement(11870..12481)
                     /locus_tag="U6309_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEN5503606.1"
                     /translation="MKKIYWAVILMCLGLTSCVEFDESTLITDDAGYVHRTVHYLRDK
                     RGRNYFPMQIKAPGERLFVFDPKASAWAAYDEEGHRVMTGAGSGGIDVCEENPNQSCR
                     TITGTFRIYNKRGIDCRSGEYPVDTKGGAKMPYCMYFYRGFTIHAAYEVPEHPSSHGC
                     VRVFPSAAKWLNEEFLRVGTKVIVLAYPDQNGVKTMSSLEKVN"
     gene            complement(12523..14196)
                     /gene="ettA"
                     /locus_tag="U6309_000136"
     CDS             complement(12523..14196)
                     /gene="ettA"
                     /locus_tag="U6309_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEN5503607.1"
                     /translation="MAQYIFTMNRVSKIVENQRFILKDISLSFFPGAKIGVLGLNGSG
                     KSTLLRIMAGVDTQYEGEARPQPGIKIGYLAQEPELDLSKTVREVVEEGVAEIKEKLS
                     RFDAISMRFAEPMSDDEMNALLMEQGELQNEIEACGGWDLERKLDVAADALRLPEWDA
                     IIGKLSGGERRRVALCRLLLSSPDMLLLDEPTNHLDAESVAWLEHYLEEFSGTVVAIT
                     HDRYFLDNAAEWILELDRGEGIPYKGNYSSWLEQKEARLSMEKKQEDALQRAIKAELE
                     WVRTSPKGRHAKNKARLARFEEMNSKEFQKRNETNEIYIPPGERLGDLVLEGEKICKS
                     YGDRILIDNFDFKLPKGGILGIIGPNGAGKSTFLKMLTGQEEPDKGVIRIGETVKLAY
                     VDQMRDNLNPNNSVWQEISDGHDIMQIGNFQMPSRAYVGRFNFKGTDQQKKISQLSGG
                     ERNRVHLAKLLKSGGNVLLLDEPSNDLDVETLRALEEAILNFPGCAIVISHDRWFLDR
                     ICTHLMAFEGDSQITFFEGNYTEYETDRKRRLGDEANRPSRIKYRKLSD"
     gene            complement(14239..15261)
                     /gene="tdh"
                     /locus_tag="U6309_000137"
     CDS             complement(14239..15261)
                     /gene="tdh"
                     /locus_tag="U6309_000137"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEN5503608.1"
                     /translation="MKSLVKAKKEPGIWMQDVPVPEYGVNDVLIKIKRTAICGTDIHI
                     YSWDEWAQATIPVPMTVGHEFYGEIVEVGKEVQGLKVGQRVSGEGHITCGFCRNCRAG
                     KRHLCRNTLGVGVNRPGCFAEYLALPATNVIALPDNITEEQAAILDPFGNAAHCALAF
                     DVVGEDVLITGAGPIGIMAAAIVRHIGARHVVITDVNDHRLELARQMGVSRAVNVKYQ
                     KLSDVANELGMLEGFDVGLEMSGNPMALNDMMKAMNHGGHVALLGIPPQETPIDWNQV
                     IFKGLVIKGIYGREMFETWYKMIAMLQSGLNISPVITHNFPVDEYQHAFQIMASGQSG
                     KVILNW"
     gene            complement(15350..16534)
                     /locus_tag="U6309_000138"
     CDS             complement(15350..16534)
                     /locus_tag="U6309_000138"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEN5503609.1"
                     /translation="MLERFINYLQNELETLKADGLYKSERVISSQQQADVKVNEREVI
                     NLCANNYLGLANDPELIAEGQAALAKYGYGMASVRFICGTQTPHKQLEQKISHFLNKE
                     DTILYSSCFDANTGLFETLLTEEDAIISDALNHASIIDGVRLCKAARYRYANNDMKAL
                     EEQLIAAKNARFRLIATDGVFSMDGILANLPAICELADKYDAMVMVDDSHAVGFMGKT
                     GRGTPEHFGVSDRIDIVTGTLGKALGGASGGYTASNQVVIDWLRQRSRPYLFSNTLAP
                     VIAHTSCVVLDKLSTNNSLAEKLKRNSHYFREGMTQLGFELIPGEHPIIPVMLGDASL
                     AGRIANRLLELGVYVVGFSYPVVPKGLARIRTQMSAALELHHLDKALKAFETVGKEFS
                     VI"
     gene            16715..17365
                     /locus_tag="U6309_000139"
     CDS             16715..17365
                     /locus_tag="U6309_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEN5503610.1"
                     /translation="MNHSARINMIKQQLRTGDVLNESILDLYDEILRHEFVPEPFSHF
                     AYSDMQIPLAHGQRMLTPLEEGTILQSLDLKGHETVLEVGTGTGFMTALLSKLCKKVI
                     SIDYYSEFTTNAKRKLEEHNCNNVELITGDACRGWLESAPYDVIVFTGAMEKLTDTHK
                     LQILPGGKLFAILGKSPVMKAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF"
     gene            17377..18744
                     /locus_tag="U6309_000140"
     CDS             17377..18744
                     /locus_tag="U6309_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEN5503611.1"
                     /translation="MRKSLFCWILTLGVSTHAFATDLMDIYQQALENDTIFKEAYDTY
                     MSSTEAIPQARAALYPQVGLGSQAGRNYQDAVAGAFSANQYYGSYLWQVKASQALFNY
                     QAWAQVAQAKASVKAAQASFNDAAQNLILRTAKAYFDVLFAKDTLDFAEAKKRANKRQ
                     YDQATQRFQVGLDAITSVYEAKAAYDQSIATVIAARNNQINQSENLRKLTNHVYETLA
                     PLKDSKIPLVKPEPNDVNQWIDTGLKQNYKLYAAKYNLEVAKENVKAISAGNWPVFSL
                     QSNASQVHNNASGNNVFIPSKQTQANIAIAMNFPVFQGGLVQAQTRQAQYNFQSTSEK
                     LEQTYRDVIVNSRIAFNTITDGISKVKADRQTVISVQNSLQSTEAQFEVGTRTMVDVV
                     NAQQRLFEAQEQLARDQYDLINSILTLKYLAGTLNVNDLEQINSWLATTRVNGFSPVD
                     NKTSK"
     gene            18762..19613
                     /gene="ttcA"
                     /locus_tag="U6309_000141"
     CDS             18762..19613
                     /gene="ttcA"
                     /locus_tag="U6309_000141"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEN5503612.1"
                     /translation="MSSNPSSVEKKLLHYTGKAIADFNMIRHGDRVMVCLSGGKDSFT
                     LLTILNQLRIKSGNKFEIFAFTLDQAQPGWNDACLRQWLAEKSIPHEILTRDTYSIVK
                     EKIPEGKTYCSLCSRLRRGIIYRYAEENGFNKIALGHHRDDLIRTLMMSILYNGDIRS
                     MPPKLLSDNKKHIVIRPLCYVQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLI
                     DQLAIENPKVPSNMLHALQSLKPSQLMDQNFWNFKNLEDGLETAQSVQCEEVFNAQEF
                     EFEDEKI"
     gene            19804..22110
                     /locus_tag="U6309_000142"
     CDS             19804..22110
                     /locus_tag="U6309_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional SulP family inorganic anion
                     transporter/carbonic anhydrase"
                     /protein_id="HEN5503613.1"
                     /translation="MIDRNIVNLRLFRIYSKRYLKFDFVAAIVVFLVAIPLCLGIALA
                     SGAPLFSGILSGIIGGIVVGIFSGSQVSVSGPAAGMAAVVLAAISQLGDFNTFLLALT
                     LAGVLQLIIGALRAGFVADYVPSNVVQGLLCSIGILLIIKQLPLAFTLSSDFDELKTH
                     LLETTEGFTVSPLLALSQHINEGALIITTLSLAILIYFDITKNKILKEIPAPILVVLA
                     GILLNELFIWANSSLAQNSPQLVNIPDTNGFFQFFSHLEYPDWSAWANPKVYLYALVI
                     CIVASLETLLNLKASERLDKKRRHSPTNQELVAQGLGNITSGLVGGIPVTSVIVRTSI
                     NIHAGSKTKFSAVLHGFFILFAVMLIPGALNKIPLSSLAAILIYTGYKLNKPAIYINI
                     FSQGSDRFIPFIVTVISIIAFNLLAGILIGLAISLFYILKSNSQARIDIIKEIYPNGS
                     TYRLILPQQMTFLNKAALVAELDTLPRRSQLIIDARHSQYIDKEILDLFNELKEELAG
                     SKQISLNFTGFQEHYKIHNYIDFITVTTYDVQTNLTPAQVLNILLEGNQRFLSDNRIH
                     RSNQIDIKYTAKTQHPIAVVLACIDSRVPVETIFDMSFGDLFCVRIAGNVINDDILAS
                     IEYACNVVGAKLIMVLGHTRCGAIQSACDGIEKGHITQLLSKIKPAVNAEKETTTERN
                     GKNQIFVNHVTELNVANTLQNIYKKSDILRTMIDSNEIGMVGAIYDVSSGKVSCKTYT
                     EELSTLDGDENKLLAQKFETILLEAKAS"
     gene            22197..23909
                     /gene="lem3"
                     /locus_tag="U6309_000143"
     CDS             22197..23909
                     /gene="lem3"
                     /locus_tag="U6309_000143"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector phosphocholine hydrolase Lem3"
                     /protein_id="HEN5503614.1"
                     /translation="MKLRYIINENKLVFTSCNMRDKIITGKKVIFSQSVAKDQTKNLS
                     SFLSERFYSVNQSHNHSIIIGSSLSHQENDIEHDTILDTSGMLVTTDTNGLVNGARVA
                     ITDGLGGGNGDQEEDDEIYRVSHSSCENFLNCNQNMDTTLSLITQPKASDKKQTAPKT
                     LQHTEASMAAFIYQNHPGKGYIGQFANIGDGLIIILDKRFKIKHMVSACHIYRGFGTW
                     TPPSLQALATTANKDALLVRQTLKLAEGDIIISMTDGVWGELKTSLIAQTNDRRDIGV
                     DKEYFKTLFDELTDSPYPSSFDIARIITSSAMSRSLERRKTLIKLINEIEQQHFHEKS
                     VKTINEVLEYFLKTGHVETAQTLKAILFEDGLSDGITYFENIEIPLEMVMHDLKSRTV
                     GDCSTINVTRIPYHLDELIRGFINYPEKHQILAPLFKARVKSEADLEEAFHRLSLEMV
                     QAEIECPISEAHFERAFKKETLDKTQAVLTHYFRISTGLDSKKNYQERLNDLSAYLSK
                     ESSLEKNDIKLLLSMLDSEIKPKTGVFQTLFGENQNKLYKAFHKKIELQFLDGEIENK
                     NELK"
     gene            complement(23916..26765)
                     /gene="ankN"
                     /locus_tag="U6309_000144"
     CDS             complement(23916..26765)
                     /gene="ankN"
                     /locus_tag="U6309_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkX"
                     /protein_id="HEN5503615.1"
                     /translation="MVKIMPNLPGLYFLQAYPREEIWRLFVDGRFWSKENGWRGYESR
                     EPGCLNAALESLCSIALQVEKSGEEFELSVDLIKRIHKKCGKKVEELQEKNPGELRTD
                     EPVSFGIPAGRASIKGIEEFLSLVFLTEGEAEFGPGKAGPFGPRFDKNYFKNLNPEQI
                     PDLAKQIYFDMCKYGHSNTNHFYLAVMKNVDVYLEKITQSYNQEIKTAETLDEKLKII
                     VKHIRMYEVLHPFRDANGRTFVNNLLNILLMQQGLPPATFYEPNVFDLYSAEELVVVV
                     KEAIFNTVEIIEQSKRKTPITLYGYHSSLEEQTKFRDMLDSPAYEKIKHMDFSDLNPE
                     KLHRKTQKCLSSLNEQYPLHRGAIYLSDPGEIKLLLSNRNESQINQQIEQGAPPIYVG
                     KTPAHLAVISGNMAMIDELIAKKADLSLQDYDGKTALHYAAECGNMQIMGKILKVVLS
                     QEDAIKVLNIKDNHGKTAFHYAAEFGTPELISALTTTEVIQINEPDNSGSSAITLAYK
                     NHKLKIFDELLNSGADISDELLDAIWARKDKETLGKIIAKNEKILLNKEAFRIAISLG
                     SVSLVKKFLRAGVDIDIPLTKDKATPLMLSINSGNPKLVSYLLKKGANTRLTDTSGNS
                     VLHYVFYSKAENREALANIITEKDKKLINQPNTNGNPPLYNAVVVNDLKMATILLEMG
                     ARVDFEDRLGNNILHSAMRRCDLPIILDIVKKDSTLLHNRNSERRNPFHQALHEMHTF
                     PSSKETEEIHFMNLSDLLLKEGVDLNKKDIKGKTILDIALSKQYFHLCVKLMKAGAHT
                     NISSPSKFLKNSDANSILERPFKFKNDLKKELDDNPLIAMAQINDLYVQIKNNRIRTP
                     TGYAPKEGVSFFKGKSSDAKAHDEVLSVLKELYDSKLTEMLGNLPGEGLEEIKRSQKF
                     FDGELKLLIKNQDISRKVDKKSIQEAVGTSLKLKW"
     gene            26927..28636
                     /locus_tag="U6309_000145"
     CDS             26927..28636
                     /locus_tag="U6309_000145"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEN5503616.1"
                     /translation="MRASQWFLVTQKETPNDAEIASHQLMLRSGMIRKLGSGLYTWMP
                     LGLRVLRKVENIVREEMNKTHAMELLMPSVQPAELWQETGRWETFGGQLLTMKDSNQR
                     EYCFGPTHEEVITDIMRNELQSYKQLPVNFYQIQTKFRDEIRPRFGVMRAREFIMKDA
                     YSFHLSIESLQETYKDMYQAYCRIFDRMGLKYRAVEADTGAIGGSASHEFQVLAESGE
                     DLIFYSDASDYAANIEQATSLKPPKANQACNETITLVDTPNQKTIDEVASFLGIASNQ
                     TIKTLIVKGKEHPMVALVLRGDDELNEVKATKHPLVHSPLSFIDEELILKTLKTPLGS
                     IGPIQLNIPVIVDHHALAMPSFVCGANQADKHFINAAWERDAKYDDAYDLRNVKEGDP
                     SPDGRGMLHCCRGIEVGHVFQLGDKYAKAMNASVINEQGQLQTMIMGCYGLGITRVVA
                     AAIEQHHDEHGIIWPQALAPFQVNIIPLNGARSQTVKEQAESLYQQLKSHGIDVLLDD
                     RNERAGVLFADNDLIGIPHRLVVSERNLEQGCIEYKSRTSSETQLINLDKVVNFIIEL
                     INK"
     gene            complement(28994..33277)
                     /locus_tag="U6309_000146"
     CDS             complement(28994..33277)
                     /locus_tag="U6309_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503617.1"
                     /translation="MKITRNQLNFKGVKEFGGCLTRNDEQIEFINKEIKFLKELIEFN
                     ILKGIDENKSQSLFDAVIEAVEKAGLDVEHELLIQFIQSTSEIIDSKSTDLPELVSLL
                     TVLIKSSNKYDKSELTRYCRLIDKLEKKDEKSLEQWIKILTILGRNVESVNGDLLFNV
                     QSRLEIHSVLLKDISSLFDYPPYPTLDAFSEKLREYSRELKLYIDSFDRDPAGSRAPK
                     VNQEGILIKSREQILNEQFDTSQIQEVIAKIESLVDGSGLSCKGQYELAQQVVFINAI
                     GRDFPLKIANETYTDLTQLGRNKLQELSDFFIMEMRRLDISKEDKLRTQLKLLAVMRE
                     QYFRSTGIFFDTTQMISLLLSLHHQEQNVLMGVSRNENLSAPPAAILAAMQWVLADGG
                     TVDLCFANHGLIAQDYLEKGDRDFFTCLGIPSSVVEANSESGTYQVGGINYSTLSDLT
                     SYRSHAVIEDEELSMDRCGYPVSSYLILNHIDFSNLDDRTFSHFSSLYDYYKGKGRVV
                     RITEIEETVGEVVGQISRFDMDVGYRVFPARKNLQEELNTQSLAERRREEAVKQYFIQ
                     SVSNIQQAVLNQLEEWQAFLHLIYPKSEWRQLDNELFAQRNELIDSLKKQWTKCLEDS
                     DPKKMYPNPYVRRDVFQRLQTRELEKAIQAYEKSVSSIWNLNRDQLKAKTENRFSSDS
                     VNALRCKYLEEVDLSEQIKWSKLSARLKKKEATQEKRRSHRHLESAVEVTGAMLSYHD
                     GDLEPYRLAFVHSQLKLWARDISKEINNSSLPASVKQGLIERANNADNFLALELLLID
                     YESRWLDKEKLSEKFRMQPIISDMLRVYKQAGFEEHKELKILKETYLDSTATQIIHHL
                     DKTLAWAKPENRGPWYWIERSAVKKAANEILVLANGVKQARDSLSKQIAIKKLYRDLC
                     KHQAQLEDVWIFSFDHKNTRDLINQTLNTMDSLTVLGRDEGGLDINFIQECKEEAQYY
                     LLGEKFSSVLEDFEKNNSQILTAHSEWQEVKRQLQQKQNESRKVYTLNEMYYVLCKKI
                     NELSISYSLLREPLVQLRGMIRTLWNSFQEKHQDIIDESKYFDFKARKIKEELEGIEG
                     YSVFNVKVKKGYTGFNEYVELIIEGEGTSALFEGFMQYKSRLTELKEAYRQLEFSLKQ
                     NRLELLNLNRIHAKHLPFLKDRIKGNPEVELFPEQCRSKVKEILTLKEWINGQIPENL
                     SQFPENMQNHFLDRNTLKSMDRNTMTMEQIDKIKDEQLKAELTELYNKMHKVGEEQST
                     SWFSTVTRRIVGLIPGMFRQEETEDDWRYQFNELQGRSDSILQSLLTKKIMTKYDALV
                     NEMVLIQSNLAQQMDSGNKKIQFLSDKILEEERKTNVIIKRFDNLNDFYDFEAKLKRF
                     KTAPPIVPVNEVQIESKNRILSCGEGAFSNLEEEFQTDSVMQPPMVFNI"
     gene            complement(33636..34421)
                     /locus_tag="U6309_000147"
     CDS             complement(33636..34421)
                     /locus_tag="U6309_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEN5503618.1"
                     /translation="MNYRVICCTPLLLTAMNLYAGSMGPVVEQTGFGGFYAGLGTGFT
                     TIFSTDEYSIVRPGFGVIRTGIHKVTNSAVLFSGQAGYGVMLNQKTYLGGKGSIYYTP
                     MENNNQISFASSRGPNELASGHDNFVRSLKPIYNIDAVLGYEIFPNFLPFVEAGVSFA
                     NVKHRFVLEGAITNLVTGTVSNYTGGATSDNYNTGYNIGIGANYQVHKNWIFSGELVY
                     HDLGTNNLTIPNFTPDRRSATHYREEKNQAVSILASVSYLIPS"
     gene            complement(34527..36338)
                     /locus_tag="U6309_000148"
     CDS             complement(34527..36338)
                     /locus_tag="U6309_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEN5503619.1"
                     /translation="MQNTAKINQLSVAFKSPDKTVLALDKLSFSLNPGETLVLLGESG
                     CGKSLTSLAMMRLLPRAGVYGIDSQINIHGEDILNLPEKMMRELRGRRLAMVFQEPMT
                     ALNPVLTIGEQIAEVLKRHRSLTKEQIKERVILLLNEVEMPQPQMRIYQYPHQLSGGQ
                     KQRVVIAMALACEPDILIADEPTTALDVTIQAQILALLKKIQKKHQMSLLLITHDLGV
                     AKTMADRICVMYAGQVVEQASVKDFFSKVSHPYVQQLLTSLPSFAKRNEKLSIIAGTV
                     PSLESMPSGCRFHTRCIYAFERCHHEEPQLQEVEQRILRCHLYPDIKELPLLDKNKIS
                     WVPSVEKEEIVFTVDDLSVHFIHKKGFLSRKKTIFKAVDGLSFTLSRGKTLALVGESG
                     CGKTTASRALLRLLPAAGGKIRYKDSDVLSMTGSALRRYRKKVQIIFQDPFSSMNPRM
                     TVGEIIAEGMHAQGMKPSYIRKQQKSLIEQVNLPITSLHRYPHQFSGGQRQRICIARA
                     LATEPDILICDEPTSALDVSVQAQILNLLKELQQQRGISYLFITHNMGVVSYIADDVL
                     VMKDGLAVEYGACEKVLRQPEHPYTRQLLNAVLDIES"
     gene            36472..38352
                     /gene="parE"
                     /locus_tag="U6309_000149"
     CDS             36472..38352
                     /gene="parE"
                     /locus_tag="U6309_000149"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEN5503620.1"
                     /translation="MSENYTAEAIEVLSGLEPVQRRPGMYTDTTRPNHLAQEVIDNSV
                     DEVLAGFASRIIVTLHEDGSIEVEDDGRGMPVDLHPQLGLSGVEVIMTRLHAGGKFSD
                     KNYSFSGGLHGVGVSVVNALSERLDVTIKRNGIIYQMTFANGDKLCELNETGVTKKRE
                     TGTVIRFWPNGKYFDTTKISVKHLTHVLRAKAVLCSGLSMTFINKITNEEMNWCYEQG
                     LTDYLQQTLPEDYLPEEPFAGAFKSDEATVDWALAWSDSANGNLNESYVNLIPTIQGG
                     THVNGLRSGLFEAMSEFCELRNLLPRGVKLTADDLWESCQYVLSIKMKEPQFAGQTKE
                     RLSSRQTSGLVSNVIKDAFAIWLNQHRNQGEAIAALAIERAQKRLKQAKQVARKRVSQ
                     GPALPGKLADCLQTDLSQAELFLVEGDSAGGSAKQARNKDYQAILPLRGKILNSWEVD
                     SSQVLASQEIHDISVAIGVDPGSSDLEGLRYGKICILADADSDGAHIATLICALFLRH
                     FKPLVKAGHVFVAMPPLYRIDAGKLIHYALDDEEKNRIVQHLSQTTKSKVNVQRFKGL
                     GEMNPIQLRETTMDPNTRRLVQLTLDDEEQTEAIMDMMLNKKRAADRKLWLETKGNLA
                     EI"
     gene            complement(38608..40725)
                     /locus_tag="U6309_000150"
     CDS             complement(38608..40725)
                     /locus_tag="U6309_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEN5503621.1"
                     /translation="MFRFLFPVSLFISSILLFSIQPMVAKAILPIYGGTPGVWTVCVL
                     FFQFILLIAYGYVWLLSQIKKPAVWRLIHMVLVVLSFTAFPLLFHPAARDGQPEWVIL
                     HNLLIQLGLPLLVIGASAPLLQFAYSQTKSKGASDPYYLYISSNLGSLGALLIYPWVI
                     ERFIGLTHQFYFWNFGFAIYSLLLVTVLFFTKYQPLMLTEKKEVDFLPWREIAYWIFL
                     SFVPCSLMLGVTLYITTDVAATPLFWVLPLALYLLSFVFTFTATPFISQKWISRNCLF
                     FLVFTILGFIFGVSQIRVWQLVLFNLLSFFILALLCHGQLFARRPKPHKLTLFYFCLS
                     IGGVLAGIFNGVLAPNWFNYVYEYPLAILLSLFVLPLPKTNRGWWVPLVVLGLLSLHY
                     FIPTVPWFKSTTTFHILAILALGIIVIWHRNKINLVLSMLILFVFLYFPPLQDKQILL
                     KERNFYGVKQVFKKDDVHALISQSTLHGMQVMNGEKASYGRSSYYGAIAPIVDILKKE
                     FHPLSATIMGLGAGTLLCQFRATDSIKVIEIDQQVIDLAKNNKLFTYIRDCSPSAEII
                     KQDGRLALVNMPDASQDLLILDAFNSDAIPVHLMTLEAFTLYKKKIVEDGVILVNLSN
                     RHLQMLPVINAIGRALNMITLFLAHKGDKKLGQFNSEWAMLTSNQSLAFQVMKGTNWK
                     FVADDDQFLWTDDYSNIIPLLKW"
     gene            complement(40982..41422)
                     /locus_tag="U6309_000151"
     CDS             complement(40982..41422)
                     /locus_tag="U6309_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN5503622.1"
                     /translation="MSKVIKKLEVALADTYALYLKTQNYHWHVTGPQFKSLHELFEMQ
                     YKELAEAVDQIAERIRIMGHKAPATFAEFNQLKTIKDGDSGLSANEMVSELAKDNMMI
                     VKDLNQAIKIAQENEDEGTVTILSDRVAAHEKAHWMLSASKEKK"
     gene            complement(41545..43194)
                     /gene="groL"
                     /locus_tag="U6309_000152"
     CDS             complement(41545..43194)
                     /gene="groL"
                     /locus_tag="U6309_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEN5503623.1"
                     /translation="MMAKELRFGDDARLQMLAGVNALADAVQVTMGPRGRNVVLEKSY
                     GAPTVTKDGVSVAKEIEFEHRFMNMGAQMVKEVASKTSDTAGDGTTTATVLARSILVE
                     GHKAVAAGMNPMDLKRGIDKAVLAVTKKLQAMSKPCKDSKAIAQVGTISANSDEAIGA
                     IIAEAMEKVGKEGVITVEDGNGLENELSVVEGMQFDRGYISPYFINNQQNMSCELEHP
                     FILLVDKKVSSIREMLSVLEGVAKSGRPLLIIAEDVEGEALATLVVNNMRGIVKICAV
                     KAPGFGDRRKAMLQDIAILTKGQVISEEIGKSLEGATLEDLGSAKRIVVTKENTTIID
                     GEGKATEINARIAQIRAQMEETTSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALIRAQKALDSLKGDNDDQNMGINILRRAIESP
                     MRQIVTNAGYEASVVVNKVAEHKDNYGFNAATGEYGDMVEMGILDPTKVTRMALQNAA
                     SVASLMLTTECMVADLPKKEEGVGAGDMGGMGGMGGMGGMM"
     gene            complement(43219..43509)
                     /gene="groES"
                     /locus_tag="U6309_000153"
     CDS             complement(43219..43509)
                     /gene="groES"
                     /locus_tag="U6309_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820700.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEN5503624.1"
                     /translation="MKIRPLHDRVVVRRMEEERTTAGGIVIPDSATEKPMRGEIIAVG
                     AGKVLENGDVRALAVKVGDVVLFGKYSGTEVKVDGKELVVMREDDIMGVIEK"
     gene            43695..45455
                     /gene="dsbD"
                     /locus_tag="U6309_000154"
     CDS             43695..45455
                     /gene="dsbD"
                     /locus_tag="U6309_000154"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEN5503625.1"
                     /translation="MKKWLLFSLLFLTSLCVHAEPLPASEVFKVSVKKIDPNTFAIQW
                     NILPKYFLYSDRIQLNSDNDDIAQLGTLRFPTPLTKTDKQGRTFKVYRNQLTLPVGVL
                     GITPGETIVNLRFQGCADDGFCYPPEVKQIKLAIDDKLALSQVDLETFHAPEAKPLEK
                     PEQDIADIFANHNWIMILLIFYGFGLLLSFTPCILPMVPVLSGIIVGHGRTATTKKAF
                     FLSLSYVLSMSVTYAIVGAVVALLGANLQISMQSPWAISLFSLIFVLLALSMFGFYEF
                     KLPDAWQSKIVGSSREQRGGHYLGAAIMGCLSTLILSPCVTAPLIGVLTYIAQTGNVL
                     LGSVTLFTLSLGMGTPLLLIGTSAGKWLPETGSWMNAVKAFFGILLLAVAIYLMARIL
                     PAGLVMGLWACLLIFSGIYSGALTKSNTNQEKLCQGIGIILLTYGLLILIGASMGSSN
                     PLQPLANLQAAPTVSNAFESAKAQSVKSVELAIKQAFGKPVMLDFYADWCASCKVMEN
                     TTFKDPRVQKALSHFIVIKVDVTANNKNDRALMQHFRVVAPPTFIFFNADGVQLNNLK
                     RVGELNADEFMQTIKSIDEN"
     gene            45545..46864
                     /gene="rimO"
                     /locus_tag="U6309_000155"
     CDS             45545..46864
                     /gene="rimO"
                     /locus_tag="U6309_000155"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEN5503626.1"
                     /translation="MNHKVGFVSLGCPKALVDSERIITQLKAQGYELVPTYEDAGVVV
                     INTCGFIDSAVQESLDTIKEAMAENGRVIVTGCLGAKADVIKNACPDVLHISGAHAYE
                     EVVNAVHQHLPPPADPFTQLIPPQGIKLTPRHYAYLKISEGCNQKCTFCIIPTMRGKL
                     QSYPMAQILTEAKKLKQAGVKELLVISQDTSAYGVDTRYQQVEWQGKTVNTRFYDLCE
                     QLGELGIWVRLHYVYPYPHVDDIVPLMRDGLILPYLDIPLQHANSRILKAMKRPASSE
                     NTLLRIASWREICPDITLRSTFIVGFPGETEEEFSELLAFLKEAQLDRVGCFKYSPVE
                     GAKANDLDNPVSEDIKEERYHRFMQVQAEISRNKLKNKIGSTQTVLIDEITEDQIIAR
                     SKSDAPEIDGLVYLPKISGITVGSFAEAMITDSDDYDLYGDLEYSLA"
     gene            complement(46934..48052)
                     /locus_tag="U6309_000156"
     CDS             complement(46934..48052)
                     /locus_tag="U6309_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN5503627.1"
                     /translation="MQRLWAIIRKEFLLMKRDPATIVIMAILPLILVCIAGYAINTYP
                     QKVPTVLINLDNTDVTRELVREIQNTGYFSFVASTNDSESAHHLLKTGKAILVLTIPV
                     NFTKAIRRNQNPALLLEDGSIDAIASGRAIIALAGLKSSFIEKMGQKGINIPLQRPDF
                     QIINHRLYDPDRVTQFYVVPGMIGLVLMLTMLLITVVVAFRDVQGGTIEYLLASPTRP
                     SEVLLGEIFSYILIGYLQLSLGLILSYYLFHVPFIGAPLLLYLCTLPYIVAELSLGLT
                     IATFCTSQFEAVQVINVFIAFSILLTGFTFPIFGMPEWAQYLGLFLPLTHFFKIMFGI
                     MLKGNNFGEIWANLWPLLAYCLTMISLATWRFKRHFVR"
     gene            complement(48067..48993)
                     /locus_tag="U6309_000157"
     CDS             complement(48067..48993)
                     /locus_tag="U6309_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN5503628.1"
                     /translation="MSKWSSKQIIIDVSGLAKSFNGINAVVNLNLQIEEGTIFGFLGP
                     NGSGKTTSLRLLSGLIIPDKGQGRCLNLDLMTQTKLIQAQIGYMPQNFCLYQNLTVYE
                     NLDFIARIYQLKNRKERLKEIIELLALSDNQKRITATLSGGWKQRVALAAALLHKPRI
                     LLLDEPTSGIDPQSRLLIWAHIQSIVSKGVTVLLSTQHMDEAERCHQLVYMSAGEIIA
                     RGSVIEIIQSTGLHSWVVKGESFSRLKDILVNNPAIQMIEKGNEMRISSIVDDIHQVL
                     DPFVLNNYEIKKTDTTLEDAFIYKIMKEGNPK"
     gene            complement(48986..49558)
                     /locus_tag="U6309_000158"
     CDS             complement(48986..49558)
                     /locus_tag="U6309_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEN5503629.1"
                     /translation="MRIGIAILVVLVCCTLQGCKKEKSPYQTTSYVESQLFIVSSPDG
                     GYIKEWYADEGQLLHKEDKIVTLDGVNSIKAPADSVMTERYYLKDEYVPPNFPIASLS
                     LPSQMKILFYVPESHLEKIKLGKKIRILLNEKKYSGKISFISNQAEYTPDAIFSEKNR
                     YKLVYKVKADLSQGLRDLLKIGQPVDVNYE"
     gene            complement(49626..50837)
                     /locus_tag="U6309_000159"
     CDS             complement(49626..50837)
                     /locus_tag="U6309_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN5503630.1"
                     /translation="MYANYYDMYQKTGIFSDEFFGQFLRTLGKCYVRCADVGECYATL
                     SKITDKDGESWYKAWYEMANTLKTKANISWEANHFITAAYAYLRAAEYYRASEFFLRA
                     NPDDPRVIPCFDQLQFCFERAMHRLHPGSQKIWIPYDDQRLSGYLFFSDQSPKATLIV
                     PGGYDSTFEENYALVPAALLHGYHILIFDGPGQGHVLRRQKLYMRADFEHVVSQVVDW
                     LDEQSELGTSSYVLIGRSFGGYLAPRAACSEKRLAALICDPGQMDIAGSLNKMLPPNL
                     MMLLKEGKADELNACFYQLFSENPMKEFYFKSRMYTHGLATPFDYLQALSAYTFVDRV
                     QEITCPTLVCDNPSDRISNRGNTLYEALRCEKTYVMFDAKLGAGMHCEADANGQFQQV
                     IFDWLDERFTQ"
     gene            complement(51105..53846)
                     /gene="glnE"
                     /locus_tag="U6309_000160"
     CDS             complement(51105..53846)
                     /gene="glnE"
                     /locus_tag="U6309_000160"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEN5503631.1"
                     /translation="MSPQSDIETLISTKSWLIEKYVAPLHHPLRESAEKLILVSDYAC
                     RQIRLLVSLLESDSCCSLWSRESYFYAIQQISLELSQAQYMQELRRFRHTHFLRLLLL
                     EIADLASTQEVMQSWSDLADSIILHTLKYIGFTLLSRYGVPRNSEGKEIQLYALAMGK
                     LGGRELNYSSDIDLIFAFNEVGSTDGEEQITNQHYFSKMVQTFVHLLQSVTPDGFVFR
                     VDLRLRPNGDSGPLVSSFAAMETYYQEQGRDWERYAMVKARVIAESLDEPFPWFERLI
                     IPFVYRRYVDFGVIESLRGMKAMIEREVQLNPLLNDIKRGRGGIREIEFIIQNIQLIR
                     GGRLPQLQVQNAMLALAVIKKEKLLPRCDALKQAYLFLRKLENILQSLNDQQTHSLPE
                     DQLKKVQIVYAMGFTDWEQFFNKLQQYQRIVSNFFHSILGKVDDYEDEKRLLNNQLFS
                     LWQGHVESNMAVNLLISLGYENAQHCYQMLHAFRHGPRCRRLSQGARIRLDRFMVLLL
                     TELTYCPKTDEVLLQVIHLLENIVGRSAYLALLTENPHALRELLFWFVNSPFISSLFV
                     SQPFLLEILLEQEKDWHPDSLSQMEQTIVEKLEHTNDVEVHEEILRQFKLTNWMVAAR
                     AELYGLCDAVRIGKFLSDVAQVIVSQVLIIASKQLCGRHPEMAQIQSRFAIIAYGKLG
                     SREMSYASDLDLVFLHSAKPSEEALVTRLTQKILHMLTTRTQMGVLYHVDTRLRPSGA
                     AGLLVSHVDAFVEYQKSQAWTWEHQALLKARILSGNSKIKNQFSQLKKSVLLLERDSK
                     LLQRDVLTMRSKIDQFQESDEVKLARGGLLDLEFLVQFLILNLRDPKFSSYTNTLSQL
                     KHLFLSNVLTKSQFTTLKKAYQYFHYLMHQKIVQPDVINDDEMQNAVSSVCKDLYRY"
     gene            53984..54736
                     /locus_tag="U6309_000161"
     CDS             53984..54736
                     /locus_tag="U6309_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN5503632.1"
                     /translation="MKFIQGLLLSFFFLVSPLHAQNEPIPLIVGVGGFYPPFIMQGTN
                     NELFGYDIATMTELCKIMKRQCNFRVMRFRQLIPALINKQIDAAISAMSITSERSNLV
                     NFSMPYLLSYSRFLTNEVHNTHQPFNLMLLKGKRIGVEQNTVFSQEIRRMGIIDPVIV
                     EFDRTEEMLEALSANKIDFVLMDNPAALYWSANSSGRLLLIGSPMLIGYGLGIAVNRE
                     NSVLLKSLNDALVEYQRSDKYKENYNKYMMNF"
     gene            complement(54980..55246)
                     /locus_tag="U6309_000162"
     CDS             complement(54980..55246)
                     /locus_tag="U6309_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503633.1"
                     /translation="MSQNWPTRDKDLQTARVIMEEYANERDSDSLGLFEIVVDQNEKR
                     MNFRLSGWVVILAKHFTSLYGVSQGDYVTRQVISRCITQGQTLH"
     gene            55544..56377
                     /locus_tag="U6309_000163"
     CDS             55544..56377
                     /locus_tag="U6309_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEN5503634.1"
                     /translation="MHKTINGAMTGIGLRAPHYAQILEKKPDIDWLEVHTENFLVEGG
                     PLLELLDSISQHYPLSFHGVGLSLGSAEGVCIAHLNRIKTLIDHFQPALISDHLSWST
                     IGNTYLPDLLPIPYNEESLTIISNNIDRVQNYLKRTLLIENPSSYLEYQTSSYSEPDF
                     LAEIIKRTEARLLLDINNIYVSCSNNKRDPYQYIQRIPHEAVQEIHLAGHSIAYQDNT
                     NFLLIDTHNNYVCPEVWDLYRYAIHYTGLVPTLLEWDTEIPSLDTLLNEARKSACYAY
                     N"
     gene            56364..57179
                     /locus_tag="U6309_000164"
     CDS             56364..57179
                     /locus_tag="U6309_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HEN5503635.1"
                     /translation="MPTIKQMQEFFLNTVLNQQDIAIFEYFCPDEITNEFRFDIYRNT
                     IFQNLRHSLEMTFPAIWKLVGKECADGLALHFIQDKNNLPTTHCLDDWGEKFPLFLKN
                     NPTVSHLLYLKDIAEIEWLKHLSYCSRDCLVFDPQKLQKHLNDRVEKLILLFNPSVFL
                     YSSSYYLKNIFDLIENPTETGSIDFQQIPSYAVISRQKNQTVIHWITHDLFHFLCHIK
                     AGASLGFAYENTLENNPDFDLLTALQFLLRNELLEDSFIHSNYHLTHANNCPE"
     gene            complement(57199..58512)
                     /locus_tag="U6309_000165"
     CDS             complement(57199..58512)
                     /locus_tag="U6309_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEN5503636.1"
                     /translation="MDSYSKLPLRIRKEIEAQQYTGELLVAGVQLGVIVLLSIINIFS
                     SAGYSPGAPVQSSSLGLWLFAILILLRLWFVYTNQLNPIFLGFFIVAEMALLLFIIWT
                     YYLQFETTPTINLKNPHINYVYVLIALRALRFEPLWVILSGLTAALGWGIIAWQTLSS
                     SGMNVITWDYVTFASTRSVYLGAVFDVILSVLLVTTIIALVLTRAKHTLFQAVEQTSA
                     AKDLSRFFDTSVVEKITTSEKSLQAGYGELRQAAIMFIDMRGFTKASVKLSPSELIGL
                     LAEYQRLLVPIIQKNNGSIDKFMGDGILASFGAVTPSNTYAADALRTVDEIVASVDVW
                     SENRRKKEQIVVGVGIGLASGEVVVGVIGYENRLEYTVIGEAANLAAKLEKHNKEEKT
                     QALTTRDTFLEAVKQGYEDKITKKELNARNVGGVGEPLDLVVLAK"
     gene            complement(58505..58777)
                     /locus_tag="U6309_000166"
     CDS             complement(58505..58777)
                     /locus_tag="U6309_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEN5503637.1"
                     /translation="MNKRPIQNTAVIAAALGAALASIYTYTDWLSSDEITVKRERCYN
                     VARAGKNDCATSQHSCAAQSTADRDPEAFIMLPKGLCERIVGGRSG"
     gene            complement(58815..59579)
                     /locus_tag="U6309_000167"
     CDS             complement(58815..59579)
                     /locus_tag="U6309_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HEN5503638.1"
                     /translation="MDKYLSANSLEGVIDNEFSMPAPRWLNTYPAGPYRFINREFFII
                     AYETDPDLLQAILPPDMELLEPVVKFEFIRMPDSTGFGDYTESGQVVPVRYKGEEGGF
                     TISMFLDCHAPIAGGREIWGFPKKLAKPKLFVEEDTLIGILKYGSIDIAIATMGYKHR
                     PLDTEKVLESVKKPVFLLKNIPNVDGTPLVNQLTKTYLTDITVKGAWTGPGSLELHPH
                     ALAPISNLYIKKIVSVSHFITDLTLPYGEVVADYLA"
     gene            complement(59621..>60662)
                     /locus_tag="U6309_000168"
     CDS             complement(59621..>60662)
                     /locus_tag="U6309_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN5503639.1"
                     /translation="NKEERLVIADNFTIPYDYLVIATGATHSYFGKDSWAPYAPGLKT
                     INDGTSVRSRIIKSFELAEIAESDEERKQFLNFVIVGAGPTGVELAGAIAELARFGIV
                     KEFRHFDPASANIILVQAAPRILPTFSEQISQKAQRYLESMGVKVLVNSMVEQIDSDG
                     VIINKERIYSKSVFWAAGVAASPASKWLQLEADPAGRVKVNDDLTVAGYSNIFAIGDT
                     AASNAWNGKPVPGIAPAAKQGGAYVAKVISKRIYNNNSRYKPFKYIHYGSLATVGRKA
                     AVAEFDRFKISGELAWWFWGGVHVFFLVGSRNRLSVILNWLWSYYTFRANNLLITDDS
                     LNKKNKSGTSEV"
BASE COUNT        19162 a        12749 c        10688 g        18063 t
ORIGIN      
        1 aatgaaaata atccaaaagc tatcactaca gataagcttg tgagcaagga tcgaagacca
       61 tttaattctt tattctgcca ttcaattgct aaacgagagc gctccatacc catagcctga
      121 ttgtatttta agtcatattc ctttttgtct tgttggtaac gaataatcat cactaaaata
      181 tcgatgagtt gagccaacgt ttctaattgc atcccttgaa ggcctgcaga tattgagttt
      241 ctataagata accaaaaaaa ctgggtcagg ttaacaacgc accacaagct atcatttaaa
      301 agactgtagt aaatatccct tcgtatttta tgattagttt tttcttgcga ttcgactaaa
      361 atgagatgcg ctaataaacc aacgttagct gaaaatcgaa gcatatacag agaaaagctc
      421 atcacaccca aagccgcatc taagggagca agatcccttg tgtttttttg tgattttgct
      481 aaatactttg ctaaaccttt accacgattt aattgaagtc ttttgtcatt taaatcagtc
      541 agtttgctat taaaaaacat taatgtggat tttatgatcg gttttgctgt gcctgactcg
      601 tttttgctca tattagtatc atcaaacaat tatgaattaa taataagcaa taaaatctgt
      661 cagaagagtg acatcaagat tacaaaattg taatctaatt cattatgata attagcattt
      721 ttatgttaaa atagaacata atttaaacac aatggcatta tgcgattatt aattgttgaa
      781 gaccataaaa gaacggctga ttttatctgt aaaggattta atgaatatca atttcaggca
      841 gatgctgtat acgatggtgc agaagcaatc tatcgaatct cagaaattaa ctacgatgct
      901 gttgttttag acgttatgtt acccataatg gatggatggg ctgtacttga aaagattcgt
      961 gaactaaaac ccgatttacc tgttttaatg ctttctgcct gcgacgatgt cgatagtcgt
     1021 gtgaaagggc ttgaaatggg tgcctatgat tatcttgtta aacctttttc cttcaatgag
     1081 ctccttgccc gagtaaaatc tttactacga agaaaaccca aagagagccc tatcttatta
     1141 aagattaatg atttaactct aaacttacaa aagcatacag caatacgagg cgacaaaaag
     1201 ttacctttaa cagcaaaaga attcatgtta ttgacgttta tgttgaaacg aagtggtgaa
     1261 gtgctttcaa ggacagtcat tgctgaacaa gtttgggata ttaattttga cacgaatacc
     1321 aatatcattg atgtagcgat caagcgttta agggaaaaag tggataatga tcatcctact
     1381 aaattgatcc ataccgtcag aggagtaggc tatgtattgg agattcgcta actcaatgtc
     1441 cattactaac cgcttattac tgatatttat tggttcaacc gtactgatat tgtccattat
     1501 cactgggtta atctatcctc caatgaaaga attactccat catagtcatt tgaataatga
     1561 gcattataat tatttattaa cacggatttg tattaaaaat ttctttgttg gactctggtt
     1621 ttctactttg ataatcattc ttgccagtta tttgctcgca aaaaaaagca tgagaccaat
     1681 taaaatattc actaaagagc ttgcatcgat taatgcctca tcactagaga aacgattacc
     1741 ttacacaggg aatcccaaag aactggaaga attagctttg acttgtaatg atatgctctt
     1801 tagaatagaa agtgcattta gccatataaa gcaattttca gccagtatag ctcatgaatt
     1861 acgaaatccc attcattacc tccgaactgc taccgaaatt accctgacta aaccacaaac
     1921 gattgaaacc taccaacatg tattacaaac gcatctcgaa gaatatcaaa gtttaaccca
     1981 acttatagat aatctgctct ttttaactcg ctgtgagcac ggccaacttc aattaaatgt
     2041 tagaacatta tctgcaaacc acttagtcag ttcaattatc gagtattatt attcaatagc
     2101 tcaagaaaag ggcatagaaa ttcagatgtt tggcgaggct cagattgaag tagatgaaca
     2161 tttatttaag cgcgttattt caaatttaat agacaacagt ctgacctata ccaacaaaga
     2221 cggaagaata ctaatcatca tagaaaaaag aacagacaac agcattcaaa tccgtataaa
     2281 agacaatgga attggtatat caaaagaaca cttacctctt ttatatcacg gattttatcg
     2341 cgtagattat agcactaaca aagaaaacgc aggtcttggt ttaggtttgg ctatcgctaa
     2401 atcaatcatg gaacaccata aaggccagct tgcgatagat agcaagatgg gtttaggcac
     2461 cactgttata ttaaccctac caggaaaaag ctttttaaag attgaataat gtatgtattt
     2521 catctatccg agaccttaag ggaacggata tacattgaac taagttattc taagaacata
     2581 gacttttccc gcctcacaca gcaacaccaa tctacaataa gtagaattgt tagagtaatt
     2641 atcaattaac tcaaattttg cttctgcaga cacgatgact tatccaccat gctaaaagca
     2701 aagtagaaaa tactcctaaa tacactccat attgtttcca tgatgatcct accagctgca
     2761 aagcatcagg gctatgaaaa atagaaaacg gtatagccag taacacatcc atgatcgctg
     2821 atcctgccac taaaccacag gcaataagag tgcctttttg ctttctattg gtagattctt
     2881 cagggttaac tttcaaccgt cctaaacgat tttgaacaaa tagggcaatc attcctccaa
     2941 taaataaagg aaccgaagag gaaagaggta aatacattcc aatggcaata cctaaaatag
     3001 ataaattaag aaagcgtttg atgtttaata tgcgattgag aaaaatagcc aataaaataa
     3061 tacatgaacc agccaacatc atattccagg gcaatgaatt tctaaatact gcttccgtga
     3121 tggctgccat taatgcggcg gtcggggcgg gtaacgattg tgttacatcc attccagcat
     3181 gtggcattac tcctgctatg ccatagacat taaataaaat ctgcatgact ggaggaataa
     3241 ccaaagcaga aataacaacg ccaagcagca acatcacctc ctgtttccat ggagtagccc
     3301 caactaattg acctactttt aagtcttggg tgttgtcgtt agcgatagcc gctatgcctg
     3361 tgactacagc acctataata atagtaatag cttctgcagc ttgaatttga tgtgaagtta
     3421 atggaagggg caatagatga ttaattgctg aaaccaataa ccatgcggca aaaagcatac
     3481 ccgagataac cactgaactc ccgggacttg cagtgactcc taccattcct gaaaaataag
     3541 ctgtaatcac tgagaataaa aaaccgacga ctaaaacata aataacacca ataaatacaa
     3601 tagttggtga aaaatcatta tccagtccaa cctgtcctaa tggaaaaatg atttgaaaga
     3661 acaaaaacag tattcctgcc atcagtccaa ttcccatcaa tataaatggc atgggaatat
     3721 ctttttctgt gcgaggatat tgttctctga acttgctctt ggaaatataa gttctgaatg
     3781 atgtcttgac acttctggtg agtggtttcg ctaattttat aaaagtccaa ataccggcaa
     3841 acagcatggc tcctatcccc aaatagcgca tttcactatt ccataacaaa gtagctgctt
     3901 gttcggcggg atattgattt aaaaaatcag gataaaactg gctggctatt ggcaaagcga
     3961 tcaaccatga aatgaccgcc ccgagaaaaa tgcttattgc catatcgtga ccaacgaggt
     4021 agcccgcacc tatcatggtt gcagagaagc cagcacccaa accaaataga gaacgtttta
     4081 caacaaacca ataattccag ctgctggcaa ttactttgaa accaatttgc agtaactcga
     4141 gaagtccgcc tacaagcccg ccgagaaaaa tgtctttaat accaaccttg tctgccgaag
     4201 atttcaagac ctctgcaatc gctcttcctt caggaaattt gagggtttgg tcattaacaa
     4261 gaattcgacg caaaggaata gaaaacaaaa ctcccaggat tcctccacaa acagcaataa
     4321 aaaaattagt gacgtaatca aagtggttcc aaaacccgat gataatgagc gcaggaatag
     4381 tgtaaacgat acctcctgca acagcctcac cagcagatgc tgctgtttgt acggcattgt
     4441 tttcaagaat ggttgaattt ttaaagaatc gcaaaatccc catggaaata atagcggcgg
     4501 gaattgaagc ggaagttaat ataccgagtt taagcgccag ataagcatta gacatggcca
     4561 gtagaacggt caaaattatc gccagaataa tcactctaaa agtgagttca gctacttttt
     4621 tattcgctgc aataaatgga gtatcagaca tcaattaccc cacactgctt tggaaatttc
     4681 tggttttacc atagcagaaa ttagtgataa tgggatatcc actgtttgct ctccataaac
     4741 ataagctgcc acctggtatg tattaaaaac aatagcaatc ccttttttgg taaaatgcca
     4801 aatactataa ttttcctgtg tcggtttcgt tccttcttta atccagtttt catctgaaat
     4861 cttcttggct gtgatttctt ttttagccag atcagcgatt ggctgcaaat aatcggaacc
     4921 tggaacaaat aaatcagaca attgtacagg ctgattcttg ataaagttga gtacctttac
     4981 tgcatttgag ggatgagcag ctcctcgatg atatatggaa atattaaatc gaacacttag
     5041 ggcattagtt gattggtaag gaatagcata tgtaatattt aaaccggttt tacctggagc
     5101 atctgcagga gtatcagcat cttcagataa ttcattcata aaactctttt gctgattagt
     5161 aataaattcc tttaccgcct gatttactcc gttactttga aatccctgag ggtattttat
     5221 atccagcaaa taatccgctg tttctttttt aataacttct gttgtaatcg cagaagcaag
     5281 aaacgaataa aataatagcc caaacatcag gccaatttta ttgattaaat tcatttgtat
     5341 cggcattgtt tatctccaat caagtaatcc tctcaggata cccattcacc agccaccatc
     5401 gtttgatgag gtaacggata agcgaaataa taacatagtg cagcgctatc ttttatagac
     5461 cataaaacca aatcagcaat cttgcctgct tcaatgctcc caatatcctt ttccattcct
     5521 aaagctctgg aagcctgata cgttactgca gataaaactt caggtataga catagagaaa
     5581 aattggcaag ccatactcat catcaataac aaagaagtcg ttggagaaga acccgggtta
     5641 gaatccgtgg caatggccat accaacacca acctgacgca ataaatcaac aggtggtttt
     5701 tgtttttctt taaggaaata aaaagcgcca ggaagtaaga cagcaaccgt attggctttt
     5761 accatattta acgcgccatt ttcatccaaa aactccagat gatcacagga taacgcacca
     5821 taacgagcag ccaaagaact ggcccccata ttagataatt gttcagcatg gcattttatt
     5881 ggtaaattta aatccttagc agcttgaaaa atttgctcag cctgccttat agaaaaagct
     5941 atggattcac aaaaaacatc cacggtatct accaaatcca tatttttagc tgcaggcaac
     6001 atttcattac aaagaaaatc gacataagcc tgactatttc ctttaaactc gggacctaca
     6061 gcatgcgccc ccagaaaagt tgtttttacc ctgactccag ccatttcccc taactgcctg
     6121 gcaaccttga gcattttcaa ctcattttgt aaatcaaggc cgtagcctga cttaatttca
     6181 acagtagtaa ccccttcatt ttttagcgca agaagtcttg gcaatgattg attaattaac
     6241 tcttcttcgg aggcatctcg tgtcatttgt actgtggata atatgccccc gcctgatttg
     6301 gcgatatcag cgtaactgac gccttgcaat tttaatcgaa actctgcggc tctgtggcct
     6361 gcataaacca gatgagtatg acaatcaatt aatcctggtg taattaattg gccgtgacaa
     6421 tcctttcttg acttggcact ttcctgaaaa tgggcaggta actggtcctc agaaccacac
     6481 cactctattc tgcctttttt tataacaatg gcttgattag aaagttgcaa ccctgtggca
     6541 tcgattgtgc ttgcattgag caataattca tcacaggcaa acattaaaat tcccaatgaa
     6601 gtacctggtg aggcggcttc aaccaggcag agtggtgagt cgtgtcatat atatcccact
     6661 ctttggatac atacgtttca ttatcaatat ctaacagcag ccaaataaaa aattctgcta
     6721 ctgtttcggg tgatatcaaa cgattatgat cttttaaacg cctataaaaa ttgacctgtt
     6781 ccggatcaag attaatcccg tttcgggcta ttgcctgcat atgagtgtcg ataattccag
     6841 gcattacgct ggcaaaggcc agtgaatcgc actcaatctg ccagcatcgt gttaacatag
     6901 ccaaggccgc tttcgataca caataagctc cccatccttt gataggaaaa taagcggcag
     6961 ctgaacctac attcaatact ctgccacctg acaatttatc atacaaaagt tgtggaagaa
     7021 aaagtgccgc atctatattg gtgtttaatg cctggtgcca ctcggcaaaa gaaatatctt
     7081 ttaaaagcag tgtaggctcc agagttcctg cattgtttat caaaccatca atttgaggtg
     7141 tggtacttaa actggctttg attaactctc tgccttgagg agtggagaca tcggcacata
     7201 aataatgaat gtttgcagac aaagaagcgg tttcttgcag taatttttcc ctgcgcccca
     7261 ctatgaacac ctgtttattg cgttttgcca aagcaaacgc caaagctctt cctatgccgc
     7321 taccaccacc agtaatgaga tacacaattt cctcatcaat atagaaaaca ataggtgaaa
     7381 taataccaga cattagactt cttgcataac ctgcacgttt tattttagaa caagaaaaaa
     7441 gcgactgaac gggctaattt tatcaaaagt aaatgaaaat cttgagtatt tagcatttct
     7501 cttggaaact agctctatca ttagagctct tgcataacct gattattttg ttttaggaca
     7561 aggcgcaaac gttttgagga gcggagttta catgcagtaa atgagcaccc agaaacgttt
     7621 gcaacgcggc aataacacta aagaaacagg ttatgcaaga ggtctattaa aaggagaact
     7681 tgatgtttga cgatttaagt aactatcggc cagccaaccc tgcgctttgg caaggcagga
     7741 aggacaccgc caaccaggaa cgtttttttc aaaaaattac ttttattgat aatcaaaatg
     7801 aattaatgac taaagacaaa aaaaccatat ttcttggatt tgccagtgat gccggaatta
     7861 aacgcaattt aggcagaaca ggagcaaaat taggccctga tcaaattaaa acccaacttg
     7921 ccaaattacc atgtcataat aataaacatt atgttgatct tggaaacgtg gtatgtgaaa
     7981 acgatgaatt ggaattatcc caatctcaat ttgctcaaat agttcatttt tgccatgaaa
     8041 atggccatca gatctgtgct ttcggcggtg ggcatgaaat agcctgggcg cactatcaag
     8101 gattgtcctc actctatccg aaattagggg ttatcaactt tgatgctcat tttgatttac
     8161 gcccttataa aaaaggtgag ttaggcaact cgggaactcc tttctctcaa attgccacct
     8221 attgcgaaga aaaaaaaatg ccttttcatt attgttgtat tggggtgcaa aaatttggca
     8281 ataccccatc tctttttgaa aaagcaaaaa aattaaatgt gagctacctc tcagcagaag
     8341 acctttatga gcaaagccag gcttggcaaa ttgcatttct tgatgatttt atccttaatc
     8401 tggaccatat ttacttaacg atatgcctgg atgttctcgc cgaatgctat gcccctggtg
     8461 taagcgcccc gcaagcatta ggtttatctc cctggcaaat aatgccactg ttgaaatacc
     8521 ttatacaaag tggcaaagta gtaagtttag atattgcaga actttcgccg ccactagatt
     8581 ccgaattaaa aactgctaga ctagcggcac ttattatcgc agaattgtta gatactaatt
     8641 gaggagtata tttcatgaag aaaactgtgc tgtggggtgc tttatttact ctaatcacta
     8701 ctcaagcgaa ctctgaagca actcagcaag cgatatcaac tcaaaattcg gcgtccaccg
     8761 gcactaatgt agtttctgag tcaacgaatc aggtacctgc cacgaatcaa aacacgcagc
     8821 aaaatcagct gaaccaacct gtacaatcaa atggacaaca accaactcaa accattcaaa
     8881 acaatcaaca aaatcaaccg agccagacag gggaacaaca gtctaaccaa aacacccaga
     8941 ataatcagca gaatcaacca gcacagcaac aaaccacaca gcagcctgct caacaacaag
     9001 taccttcaca acaaacagcg ccagttatta attgtgatta caagatttcc gccgaaacaa
     9061 aagctataga gcaatccttg gttttaacct ggtcagagaa agcaataatt cagtcatttg
     9121 attttgatcc ggctactgta gatgcccaat tgcaaaaatt acaagcctgc tttacagatc
     9181 aaggatggct aagctttaat tcagcattgc aaaaatcagg taatctagat gccattaaat
     9241 cacaaaagtt gcatgttagc agccaaattg atggtcagcc acaagtgact gaagcgatag
     9301 aaaatcaatg gaaaattatc ataccattac aagttgtcta tcaaaatgac aaggagaagg
     9361 taacccaatt acttaatgtc aacgtaactg ttggacgaaa aattaccggt gatttaggca
     9421 tcaaccaaat gatcgctgta ccccgcacag caaccaatac ctcacaaacc aattcaccgt
     9481 ctaatacaaa cccagcagac gcaaatacct cgacccctca aacacctggt gacacaacaa
     9541 ataatgctac ccaaaccaat acacaaacac agactgggac accaaccaac tcacctccga
     9601 gtggctccac aacaaatacc cagaattaaa ttcaacaatt agtgcggcat tccattaaga
     9661 atgctgccaa aatcacggaa atatcatgcg attaatattt acaggagtat tatgcctgtt
     9721 gactctcaat gcccaggcga atgtgataca gtattttgca ggaattagtt ataacaaccc
     9781 cgctgattta ttcaaagtaa aaaatggtat cttgcttgtt ggaggaaccg gctcttatgc
     9841 cgatttgcaa tttaagggaa gcgccttaaa tttcaatacg tttcaatacg attcaggggt
     9901 taatcactcg cgtacttata ttgtttggcc atatggtcga gttgccaagc gcctcaatga
     9961 taagactgta gttgcagtag atcttaccga accttttaat tccaatcttg attggggcaa
    10021 cgacgctttt acaagatatg ctgcgaccca aaattacctg accgatgtgg atctaagccc
    10081 caaaatatcc tatgcgatta gcaaaaaatt acaaattggt ggtggtataa actttaatgt
    10141 actactaaag aatgaagtca attgggcttt tcctaccggc caatcaactt atgcgaattt
    10201 aattaaccga tcctctagtt ttggtgtggg ctataatttg gggattaatt atgcagtgaa
    10261 tgataccaat ttccttggta tcacttatta ttcaagaatt cgccaaaata cttccggtac
    10321 cagttatctg ggattagctg ccaaccctga tttccaattc ggtttttata tgccagccac
    10381 aacggttgcc agttatgtcc atatttttaa tcccaaatgg ctcatcaacc tacaggtttt
    10441 tcaaagcgaa tggaacgcca atcaaaaagt gagactttat aacacagcag caccacctcc
    10501 atttactaat tttatttttg atatgcattt tgatgcctct tatgcttatc ttgccgcaat
    10561 acgtaaacaa gtctctgata aattaggcat tgcgctagca ggcatgatcg atgatggtcc
    10621 tgaagaagat ggccttagaa ccatcgtttt cccttctgat acccaatatt ttcttggatt
    10681 gattggtgac taccgtttta cggaacatgc ttccatggaa cttatcttgg gacatgtgta
    10741 ttctaatccc agcattcaaa acaaagccaa ggtaaacaat gtacccgttc cctttactac
    10801 aggccgggtg acaattaatg ccaatgtttt ggatcttaaa gtcaaaatcg aggggtgatc
    10861 ttccaggtgt aaccacctgg cacttcctcc aagagtttta tttttgaata gtcctctttt
    10921 taaatagcca ataataagtc catgatcctg ctatcagccc aatcacaaaa tatactggat
    10981 cggaattaaa ggtagtcaag gcagtaatag atgggcctgg gcagtatcca gccacacccc
    11041 aaccaattcc aaaaatgata cttcccaaga ttaatttttt ctcaatgttt ttcttctcgg
    11101 gtaaataaaa ttgattacac aatattggtt tatcaaattt gtttatcagt ttgtacccca
    11161 aaaaagttac ggtgacagca acagccatta caatcagtaa actcggatcc caaaatccat
    11221 tgatatcaag aaaatttaat accttgttag gattgatcat atttgaaata gtcaacccgg
    11281 ctccaaataa caaaccacat agaaatgaca tgatgttata cattaatata atcctcccac
    11341 atgtcttatc agatacacag taatcattgc agataagata aaaagcagtg tcgctataaa
    11401 ggagcgcttg gaaaatctgg ccagtccaca taccccgtgt cccgaagtac aaccttggcc
    11461 cattcgggtt ccaaaaccca ccaaaaatcc accaattagt aacaggaaaa ccggataatg
    11521 tgttcttaat ggaaactgga taacagggct tatgaaatag atcaatcctg cagctgccaa
    11581 accaagtaaa aaggcaatac gccagactgg gaatggtttt tcaggtggaa agagtccgtg
    11641 taccattcca ctgatgcctg ctatacggcc gtttaaccat aaaaaaagga cagcacttaa
    11701 gccaatcagt aatccaccaa tggcacttag ataaggagta aaagaggtta tataatccat
    11761 tatttacatt tccgtttcat ttacaatgaa taacacctga attgagacac tttaaaaaat
    11821 taaagtgtct ctcaaattac attgatgttt taaaacaata gtccaaattt taattcactt
    11881 tttccaatga ggacatggtt ttcaccccgt tttgatcagg gtaggctaat acgattacct
    11941 tcgttccaac tcgcaagaac tcttcgttca accatttggc agcactaggg aaaaccctta
    12001 cacatccatg acttgatggg tgctctggaa cttcataagc cgcatgaata gtgaaccccc
    12061 tgtaaaaata catgcaatag ggcattttag ccccgccttt cgtatcgaca gggtattcac
    12121 ccgaacgaca gtcaatacct cgtttgttat aaatcctgaa tgttccggtt atcgtcctgc
    12181 aagattgatt tggattttct tcgcaaacat ctattccacc agaaccagct cccgtcatta
    12241 cacgatgacc ttcttcatcg taggcagccc aggcagacgc tttaggatca aaaacaaata
    12301 atctttcgcc cggtgcttta atttgcattg ggaaataatt acgtcctctt ttatccctta
    12361 aataatgaac ggtcctatgt acataacccg catcgtcagt aattaatgtt gactcatcaa
    12421 actcaacgca cgaagttaat ccaaggcaca ttaaaataac tgcccaataa atttttttca
    12481 tttagatttt atctccacaa tagtaaaacc tggtggcctt aattaatcgc ttaattttcg
    12541 atacttaatt cgtgatggtc tgttagcttc atcacccagt cgacgctttc tatccgtttc
    12601 gtattcagtg taattacctt cgaaaaaggt tatttgtgaa tcaccttcaa aagccatcaa
    12661 atgagtgcaa attctatcta aaaaccatct atcgtgagaa atcactatag cacaacctgg
    12721 aaaattaagg atggcctctt ccaaagctct caatgtctca acatccaaat cattactggg
    12781 ttcatcgagt agcaaaacat tcccgccact tttcaataat tttgccaaat gaacccggtt
    12841 acgctccccg ccagacaatt gagaaatttt cttttgctga tccgtccctt taaaattaaa
    12901 acgcccaaca taggctcgtg aaggcatttg gaaattacct atctgcataa tgtcatggcc
    12961 atctgaaatt tcttgccaca ccgaattgtt ggggtttaga ttgtcccgca tctgatcaac
    13021 ataagccaac ttcactgttt ctccaatacg aatgacgccc ttatctggct cttcctgccc
    13081 agttaacatt tttaaaaaag tagacttacc tgccccattg ggaccaataa ttcctaaaat
    13141 accacctttg ggaagtttga aatcaaaatt gtctattaat atcctgtcac cataagattt
    13201 gcatattttt tcaccttcca gaaccaaatc acctaatcgc tcaccgggcg ggatatatat
    13261 ttcattggtt tcattacgct tttggaattc tttagaattc atttcttcaa accgagccaa
    13321 gcgggcctta tttttagcat gacgtccttt gggtgaggtt ctaacccatt ccaactctgc
    13381 tttaatagca cgttgcaaag catcctcttg ctttttctcc atactcaacc gtgcttcttt
    13441 ttgttcaagc caggaactgt agttcccctt atacgggatg ccttcacccc gatccaattc
    13501 taaaatccac tccgcagcat tatccaggaa atacctgtca tgggtaattg caacaacggt
    13561 accagaaaac tcttcaagat aatgttctaa ccaggcaaca ctctctgcat ccaaatggtt
    13621 ggtaggttca tcaagaagca acatatcagg acttgatagc aacaatcgac aaagagcaac
    13681 acgtctgcgt tcaccacccg acagttttcc tattatggca tcccactcgg gtaatcttaa
    13741 cgcatcagca gccacatcga gctttctctc cagatcccaa ccgccacaag cttctatctc
    13801 attttgcagc tcaccttgtt ccattaacaa ggcattcatt tcatcatcgc tcattggttc
    13861 tgcaaaacgc atactaatcg catcgaaacg agacagtttc tctttaattt cagcgacacc
    13921 ttcctcaacc acttcacgaa cagttttact taagtcaagc tcaggttctt gagcaaggta
    13981 tccaatttta attcctggct gaggtcttgc ttcaccttca tattgtgtat caacaccagc
    14041 cataatccgc aatagagtag attttccaga accatttaat cctaatacgc caatttttgc
    14101 cccgggaaaa aaacttaatg aaatatcctt taaaataaac ctttggttct caacaatctt
    14161 gctcactcta ttcatagtga atatatactg cgccataatc gctccaactc tctataatca
    14221 gccttttatc aaacaatact accagttcag tataactttc cctgactggc ccgatgccat
    14281 aatttgaaat gcgtgctgat attcatctac tgggaaatta tgagtaataa caggagaaat
    14341 atttaaaccg ctctgtaaca tagcaatcat cttataccag gtttcaaaca tctctcgacc
    14401 ataaataccc ttaataacca gtcctttaaa aataacctgg ttccaatcta taggggtttc
    14461 ttgtggtggg atacccaata aagccacatg accaccatga ttcatcgctt tcatcatgtc
    14521 gtttaatgcc atagggttcc cggacatttc cagaccaaca tcaaaaccct cgagcattcc
    14581 tagctcatta gccacatcac ttaatttttg gtatttgaca ttaaccgctc ggctgacacc
    14641 catttgcctg gccagttcca atcgatgatc gttaacatcg gtgataacaa catgtcgtgc
    14701 accaatatgt cttacgatgg ctgcggccat tattcctatg ggacctgcac cagtaatcaa
    14761 aacgtcttcg cctactacat caaatgccaa tgcacaatga gcagcatttc caaaaggatc
    14821 gagaatagcc gcttgctctt ctgtaatgtt gtcaggaaga gcaatgacat tggtagcagg
    14881 aagcgccaga tactcagcaa agcatcctgg tcgattcacc cccactccca gggtattacg
    14941 acataaatga cgcttgccgg cgcgacaatt tctgcaaaaa ccacaggtaa tatgaccctc
    15001 accagaaact ctctgtccaa ctttcaaacc ttgtacttct tttccaacct ctactatttc
    15061 accataaaat tcatgcccta ccgtcatagg aacaggaatt gtggcttgcg cccattcatc
    15121 ccaggaataa atatggatgt ctgtaccaca aattgcagtc cttttaattt ttattaaaac
    15181 atcgtttact ccatattcag gaacaggaac atcctgcatc caaattccag gctctttttt
    15241 tgctttaacc aatgatttca tgtcatacct caaattaatt aggtaatacc accatttcgc
    15301 aattcatcct aaaaaaatat caggttgtga aatgtagagt aatttagttt cagattacag
    15361 agaattcctt accaaccgtt tcaaacgcct tcagggcttt atccaaatga tgcaattcaa
    15421 gggctgcaga catttgggta cgaatgcggg ccaaaccttt gggtaccact ggataggaaa
    15481 accctacaac atagacaccc agctccagca accgattagc gattcgcccg gccaaactgg
    15541 catctcctaa catgacagga ataataggat gttcgcccgg gattaactca aaacctaact
    15601 gagtcattcc ttcacggaaa tagtgactat tacgctttaa tttttcagca agggagttat
    15661 ttgtggacaa tttatcgagc accacacaag aagtatgcgc aataacgggg gccaatgtat
    15721 tggaaaacag ataaggcctg gatcgttggc gcaaccaatc aatgacgact tgatttgacg
    15781 cagtatatcc tcccgatgcg ccccctaagg ctttaccaag agtaccagtt acgatatcaa
    15841 ttctatcact aactccaaaa tgctctggtg ttcccctacc tgtcttgccc atgaaaccaa
    15901 cagcatgaga atcatcaacc atcaccatgg cgtcatactt gtcagccagt tcacaaattg
    15961 caggcaagtt agccaatata ccgtccattg aaaaaacgcc atctgttgcg atcaaacgaa
    16021 atctcgcatt ttttgctgca attaactgtt cttccagggc cttcatatca ttattggcat
    16081 atcgataacg tgcagcctta cacagcctga ctccatcaat aatactcgca tgattcaggg
    16141 catcactgat aatggcatct tcttccgtca ataatgtttc aaataatccc gtattggcat
    16201 caaaacagga agaatacaat atagtgtctt ctttatttaa aaaatggcta attttttgtt
    16261 ctagttgctt atgcggggtt tgtgttccac aaataaaacg aacagaagcc atgccatatc
    16321 cgtacttggc taaagcggcc tgtccttcag ctattaactc aggatcattg gccaaaccca
    16381 agtagttatt tgcacataga ttaataactt ctctttcgtt aactttcacg tcagcttgct
    16441 gttggctgga aataacacgc tctgatttgt acaatccgtc tgccttgagt gtttcaagtt
    16501 cattttgtaa ataattaatg aatcgctcaa gcactatagt ctcctgtatt tctggataaa
    16561 ttcataaata ataccaaatt ttaaacattc tcaccatgag atattatcaa gaaaatttca
    16621 ctttcaaaca ttaatgctta gacataagct gaattcttgc taaaattgct ccaaagtttt
    16681 aacctatcca atgattatta agacggtaga gcaaatgaat cacagcgcac gcataaatat
    16741 gatcaaacaa cagttacgaa ctggtgatgt attaaacgaa tccatccttg acttatacga
    16801 tgagatctta agacatgagt ttgttcctga acctttttct cattttgctt attcagacat
    16861 gcaaattcct ttggcacacg gtcaaaggat gttaactcca ttggaagaag gtacaatttt
    16921 acaatccctt gatcttaaag gccatgaaac cgtgttggaa gtgggaacag gaaccggttt
    16981 tatgaccgct ttactcagta agttatgtaa aaaagtgatc agtattgact attattctga
    17041 atttactacc aatgcaaaac gcaaacttga agagcataat tgtaataatg ttgaattaat
    17101 taccggagat gcttgtcgtg gatggttgga aagtgcacca tacgatgtta tcgtattcac
    17161 tggtgccatg gaaaagctga cagataccca taaactacaa attttacctg gtggcaagtt
    17221 attcgcaatc cttggtaaat cccctgtaat gaaagcttat ctttttcagt tggatcataa
    17281 tgcaatctgg acagaatcca tgctttttga aacggatata cctcctctgg tagatcaatt
    17341 gaaaccgaaa gaatttgttt tctagggaaa gctcgaatga gaaaatctct gttttgttgg
    17401 attttaactt taggtgtttc aacccatgcg tttgcaacag acctaatgga tatctatcaa
    17461 caagctctgg agaacgatac catttttaaa gaggcatatg atacctatat gtccagtact
    17521 gaagccattc ctcaagctcg agcagcactt taccctcaag taggccttgg ttcacaggct
    17581 ggccgcaatt atcaggatgc tgttgctgga gcgttcagtg cgaatcagta ctatggaagc
    17641 tatttgtggc aagtcaaggc ttctcaggca ctctttaact atcaggcatg ggctcaggta
    17701 gcccaggcta aagcgtctgt taaagcagca caagccagtt tcaatgatgc agctcagaat
    17761 ttaattctaa gaacagccaa agcctatttt gatgtcctgt ttgccaaaga caccttagac
    17821 tttgccgaag caaaaaaacg ggcgaacaaa agacaatatg atcaagcaac tcaacgtttt
    17881 caagttggtc tggatgccat tacttctgta tatgaagcga aggcagcgta tgatcaatcc
    17941 atagcgacag ttatcgccgc acgcaataat cagattaacc agagtgaaaa tttacgaaaa
    18001 cttactaatc acgtatacga aacactcgcg ccactcaaag acagtaaaat tcctcttgtt
    18061 aaaccagagc cgaatgacgt gaatcaatgg atagataccg ggcttaagca aaactataaa
    18121 ttatatgctg caaagtataa tcttgaggtg gcaaaagaga atgtaaaagc gatctctgcc
    18181 ggtaactggc cagtattttc gctacagagc aatgccagtc aggtacataa taacgcatct
    18241 ggcaataatg tttttattcc atcaaagcaa acccaggcta atattgcaat cgcaatgaat
    18301 tttcctgttt ttcaaggcgg cctggtccag gctcagaccc gacaagctca atataacttt
    18361 cagtctacga gtgaaaaact ggaacaaacg tatcgtgatg tcattgtaaa cagtcggatt
    18421 gctttcaata ccatcactga tgggataagt aaggtaaagg cagacagaca aacagtcatc
    18481 tccgtgcaaa attctttgca aagtactgaa gctcaatttg aagttggtac tcgaaccatg
    18541 gtagatgtgg tcaatgctca acaaagattg tttgaagcac aggaacaatt agcccgggat
    18601 caatacgatt tgattaattc catccttacg ctgaaatacc ttgcagggac attaaacgtt
    18661 aatgatctcg aacagattaa ctcatggtta gcaactacac gtgtcaatgg attttcaccc
    18721 gtagataaca aaacctccaa ataacaaatt taggttttct tatgtcttcc aatccttctt
    18781 cagttgaaaa aaaattactc cattatacag gtaaagccat agctgatttt aatatgatcc
    18841 ggcatggtga cagagtcatg gtatgtctgt ctggcggaaa agattcattc acactattaa
    18901 caattcttaa ccagctcagg atcaaatcag gaaataaatt tgaaattttt gcgtttactc
    18961 tggatcaagc ccagccaggg tggaatgatg cctgtttgcg tcaatggttg gctgaaaaat
    19021 ccattcctca tgaaatacta acccgagaca catacagtat agtgaaggaa aaaattcctg
    19081 aaggcaaaac ctactgttct ttgtgttctc gcttgagacg tggaatcatc tacagatatg
    19141 ctgaagaaaa cggatttaat aaaatagcat taggtcacca tcgtgatgat cttattcgta
    19201 cattgatgat gtcaattcta tacaatggcg atatacgttc catgccaccc aaactactga
    19261 gtgataataa aaagcacata gtcatccggc cactgtgcta tgtacaggaa aaagatatta
    19321 ttacgtttgc atcagaacaa gcctttccaa ttatcccatg caatctttgt ggctctcaag
    19381 aaaatctaat gcgcaaaaaa gttgcctcac tcattgatca gcttgcgatt gaaaacccca
    19441 aagtccccag taacatgctt catgcgctgc aaagtttgaa gccaagtcaa ttgatggatc
    19501 aaaatttttg gaattttaaa aatctggaag atgggcttga aaccgcgcag tcagttcaat
    19561 gtgaagaagt atttaatgca caggaatttg agtttgagga tgaaaaaatt tgacttatga
    19621 aaattattat ctaatatttc ttttcatttt tgcaatataa tgaaataatt gacacgtaaa
    19681 ataagcttat tattttgaca aatggaatta aatatataaa aataggcttt caaattatca
    19741 cattaactgt gactttgttt cattaacccg atactgaatt caaaataaag gaacttttag
    19801 attatgattg atagaaatat agttaacctg cgtctatttc gtatctactc taaacgttat
    19861 ttaaaatttg attttgttgc cgcaattgtc gttttcctcg tggcgattcc tttatgctta
    19921 ggtattgccc tggcttctgg cgcacctctt ttttctggta tattaagtgg aatcattggc
    19981 ggtattgttg tgggtatatt tagcggctca caggtaagtg tcagcggacc tgctgcgggt
    20041 atggcagccg tcgtattggc agcaatctcg caacttggtg attttaatac ctttttattg
    20101 gctcttactc ttgcaggtgt tttacaactg ataataggtg cgttaagagc aggattcgtt
    20161 gcagattacg ttccttccaa cgtagtacaa ggcttgctgt gttcaatcgg gatcttgcta
    20221 atcattaagc aattgccact cgcattcacc ctctcatcag actttgatga acttaaaacg
    20281 catttattgg aaacaactga aggatttaca gtaagccctt tgttggcttt gtcgcagcat
    20341 attaatgagg gcgcactcat cattactacc ctttcattgg ctattttaat ctattttgat
    20401 ataaccaaga ataaaattct gaaggaaatc ccagccccta tccttgtagt tctggcagga
    20461 atactgttaa atgaactgtt catctgggca aactctagcc tggcacaaaa ctcgcctcaa
    20521 ctggttaata tcccagatac caatggtttt ttccaatttt tcagccatct cgagtaccct
    20581 gactggtccg cctgggctaa tcctaaagtc tatctctatg cactagtcat atgtatcgtt
    20641 gcttctttgg aaacgttgct taatctcaaa gcgtcagaac gattggataa aaaaagaagg
    20701 cacagcccaa ctaaccagga attagttgct caaggcttgg gtaatataac atcaggtttg
    20761 gttgggggta ttcctgttac atcagtcatt gtcagaacat ccattaatat ccatgctggt
    20821 tcaaaaacca aattttcagc cgtactccat ggttttttca tcttgtttgc tgtcatgtta
    20881 atacctggtg cattgaataa aatccctttg tcttctctcg ctgcaatttt aatttacact
    20941 ggctataaac taaataaacc agccatctat atcaatatat tttcacaagg gagcgatcga
    21001 ttcattcctt ttatagtaac cgtgatcagt attattgcct tcaatcttct tgcaggtata
    21061 ttaataggtc tggctatcag tctcttttat atattaaaat ccaatagtca agctcgcatc
    21121 gatatcatta aggaaattta tccaaatggt tcaacatacc gtttgatact tccccaacaa
    21181 atgacttttc ttaacaaagc tgcgttggtc gctgaattgg atacattgcc cagacgttct
    21241 caattgatta tcgatgcgcg tcattctcaa tacattgata aagaaattct ggacttattt
    21301 aatgaattga aagaagagtt ggcaggcagt aaacaaattt ctttaaattt caccggattt
    21361 caggaacact ataaaattca taactacatc gatttcatta cggttacaac ctatgatgtt
    21421 caaactaacc tgacgcccgc acaagtatta aacatactgc ttgagggaaa tcagcgcttt
    21481 ttaagcgata accggattca tcgatcaaac caaatcgata taaaatacac tgcaaaaaca
    21541 caacatccaa ttgctgtggt tttggcctgt atcgattcaa gagttcctgt agaaaccatc
    21601 tttgatatga gtttcggtga tcttttttgt gtccgcatag caggcaatgt gattaatgac
    21661 gatattttgg ccagcataga atacgcctgt aacgtagttg gtgcgaagct tatcatggtt
    21721 ctgggacata cccgatgcgg cgccattcaa tctgcctgtg atggtattga aaagggtcat
    21781 atcacccaat tgctctctaa aattaagcct gcagttaatg cagaaaaaga aactacaaca
    21841 gaacgtaacg gaaaaaatca aatttttgta aatcatgtca ctgaattaaa tgttgcgaat
    21901 accttacaga acatttataa aaaaagtgat attttacgga ctatgattga cagcaatgag
    21961 atagggatgg ttggtgctat ttatgatgta agcagtggaa aagtgagctg taaaacatat
    22021 accgaagagt tatccacttt ggatggagat gaaaataagc tcctggctca aaaatttgaa
    22081 accattcttt tagaagccaa agcaagttaa acttttttga aattgtaaaa acaccggaaa
    22141 gaaataaatt tctcaagcca ggtataagat tttctacaag tcattaagcc tggcgtatga
    22201 agttacgcta tattattaat gagaataagc ttgtttttac gagttgcaat atgagagata
    22261 aaattatcac tgggaaaaaa gttatttttt cacaatccgt tgcaaaagac caaacaaaaa
    22321 atttatcaag ctttttatca gaacggtttt actctgtaaa tcaaagccat aatcattcca
    22381 ttattatcgg ttccagtctt tctcaccaag agaatgatat cgaacacgat accatactcg
    22441 atacatcagg catgttagtg actactgata caaatggcct cgttaacggg gcaagagtcg
    22501 caattactga cggtctgggt ggtggaaatg gcgatcaaga agaagacgac gagatataca
    22561 gagtatctca ttccagttgc gagaattttt tgaattgcaa ccaaaatatg gatacgactc
    22621 taagcttgat tacccaacca aaggcctctg ataaaaaaca aacagcaccg aaaacgttgc
    22681 agcatacaga agcgtcgatg gctgctttta tttatcaaaa tcatccggga aaaggataca
    22741 taggtcaatt tgcaaatatt ggtgatggac taattattat cctggataaa cgttttaaga
    22801 taaagcatat ggtatccgca tgccatatat atcgcggatt tggtacctgg actccaccat
    22861 ccctgcaagc cctggcaaca actgcaaata aagacgccct gcttgtcaga caaacactta
    22921 aacttgcaga aggcgatatt ataatatcaa tgactgatgg ggtttggggg gagctgaaaa
    22981 ccagtttgat tgctcaaacc aatgaccgtc gagatatagg agtggataaa gaatatttca
    23041 aaacgctatt tgacgaattg acagactccc cctacccttc ttcatttgat attgctcgca
    23101 tcattaccag tagcgcaatg tctcgaagtt tggaacgcag aaaaacatta ataaaattaa
    23161 ttaatgaaat agaacaacaa cacttccatg aaaaatctgt caaaaccatt aatgaagtcc
    23221 tggaatattt tcttaagaca ggccatgtgg aaacagcaca gacattaaaa gcaattctct
    23281 ttgaagacgg tttgagtgat ggaataactt attttgaaaa tattgaaatc ccgctggaga
    23341 tggtaatgca cgatttaaaa agtcgtaccg tcggtgattg ttcaaccatc aatgtaacgc
    23401 gcattcccta tcatttagat gagctcatac gaggttttat caattaccct gagaaacatc
    23461 aaatcctggc gccactattt aaagcaaggg taaaatctga ggctgattta gaagaggcat
    23521 ttcatcgcct gtcacttgaa atggtacaag ctgaaatcga gtgcccaatt tctgaagctc
    23581 attttgaacg tgcttttaaa aaagaaaccc ttgataaaac tcaggctgtc ttaactcact
    23641 attttcgtat ttcgactggt ttagatagta aaaaaaatta tcaggaacga ttaaatgatc
    23701 tgagtgctta cttatctaag gaatcctctc tcgaaaaaaa tgatattaaa ttgctattat
    23761 ctatgctaga tagtgaaata aaacctaaaa ccggggtttt ccaaacattg tttggggaaa
    23821 accaaaataa attatacaag gcatttcaca aaaaaatcga gttgcaattc ttggatggtg
    23881 aaatagaaaa taaaaatgaa ttgaaatagc agttcttacc attttaattt caaggatgta
    23941 ccaactgctt cctgaataga ctttttatcg actttcctcg aaatgtcctg gtttttaatt
    24001 aataatttta gttcgccgtc aaaaaatttc tggcttcttt tgatttcttc taatccttca
    24061 ccaggaagat taccgagcat ctctgtgagt ttactatcgt agagttcctt taaaacagac
    24121 aaaacctcat catgtgcttt ggcatcgctt gattttcctt taaaaaagga tactccttct
    24181 ttcggggcat aacctgtagg agtcctaatc ctgttatttt tgatttggac gtacaaatca
    24241 ttaatttgtg ccatagcaat tagcggatta tcatcgagct ctttttttaa atcgttctta
    24301 aatttaaaag gacgttccaa aatagaattg gcatcggagt ttttaagaaa ttttgatgga
    24361 gaagatatat tggtatgggc acctgctttc atcagtttga cacataaatg aaaatactgc
    24421 ttggataatg cgatatctaa tattgttttt cctttaatat cttttttatt taaatcgact
    24481 ccttctttta aaagtaaatc acttaaattc atgaagtgta tttcttcggt ttcttttgag
    24541 gatggaaacg tatgcatctc atgcaatgcc tgatgaaaag gatttcttct ttcagagttt
    24601 ctattatgaa gtaaggtgct atctttcttg acaatatcca atataatcgg caaatcacat
    24661 cttctcatgg cactatgcaa aatattattt cccagcctgt cttcgaaatc gacccttgcc
    24721 cccatttcta ataaaattgt tgccattttc aaatcattaa ctaccacagc attataaaga
    24781 ggtggatttc cattagtatt tggctgatta attaatttct tatctttctc agttataata
    24841 tttgctaaag cttccctgtt ttctgccttt gaataaaaaa cataatgcag gacagaatta
    24901 ccactggtat cggttaaacg tgtattagct ccctttttca ataaataact gaccaatttg
    24961 ggattaccgg aatttataga taacattagc ggtgttgctt tatctttagt taagggtata
    25021 tcaatatcga ccccggcgcg taaaaatttt ttaaccagac tgacacttcc taaagaaata
    25081 gctatcctga aggcttcttt attcaaaaga attttctcat ttttggcaat tattttacct
    25141 aatgtttctt tatccttccg agcccatatc gcgtccaaaa gctcatcact gatatcagca
    25201 ccggaattta atagttcatc gaaaattttc aatttatgat ttttatatgc caaagtgatt
    25261 gctgaagaac cgctgttatc cggttcatta atctgaataa cctctgtggt tgtcaaggct
    25321 gatattaatt ccggggttcc aaactcagca gcataatgga atgctgtttt gccatgatta
    25381 tccttgatat tcagtacttt gatggcatct tcctggctta gcacaacttt tagtattttt
    25441 cccataattt gcatatttcc acactctgca gcatagtgaa gagcggtttt tccatcataa
    25501 tcctgaagag ataaatccgc tttttttgcg ataagctcat caatcattgc catatttcct
    25561 gatatgacag ccaaatgagc aggagtttta ccgacataaa taggcggtgc tccttgctct
    25621 atctgctgat taatctggga ttcattgcga tttgacaata ataattttat ctcaccggga
    25681 tcactaagat agattgcacc tctgtgtaat ggatattgct catttaatga tgataaacat
    25741 ttttgagtct tccgatgcag tttctcaggg tttaaatctg aaaaatccat atgtttgatt
    25801 ttttcatacg ctgggctatc cagcatgtcc cgaaatttgg tttgctcttc caagcttgaa
    25861 tgatatccat ataaagtaat gggagtcttt cttttacttt gttcaataat ttctaccgta
    25921 ttaaaaattg cctccttcac aacaacaact aattcctcag cgctatataa atcaaagaca
    25981 ttaggttcgt aaaatgtcgc tggcggtaaa ccttgttgca ttaaaagaat atttaataaa
    26041 ttattgacaa aggtacggcc attggcatct cgaaaagggt gcaatacttc gtacattcga
    26101 atatgcttaa cgataatttt aagtttttca tcaagagttt cagcggtttt tatttcctgg
    26161 ttgtaagatt gggtaatttt ttccaggtaa acatccacat ttttcattac tgcaagataa
    26221 aagtgattgg tattactatg cccatatttg cacatatcga aatagatttg cttggcaaga
    26281 tccggtattt gttctggatt cagattttta aaataattct tatcaaagcg tgggccaaat
    26341 ggacctgctt tgcctgggcc aaattcagct tcgccttctg taagaaatac caggctcagg
    26401 aattcttcaa tacccttaat cgaggcccgg ccagctggaa tgccaaatga aacaggttca
    26461 tctgttctga gttcacctgg atttttttcc tgtaattcct ccaccttttt tccacacttc
    26521 ttgtgtatcc tctttattaa atcaacgctt aattcaaatt cctctcctga tttctcaact
    26581 tgcaatgcaa tagaacataa actctccaga gcggcgttaa ggcatcctgg ctctcgcgac
    26641 tcatagcctc tccatccatt ttcttttgac cagaagcgtc catcaacaaa taatctccat
    26701 atttcttcgc gtggataggc ttgaagaaaa tataatccag gtagatttgg cataattttt
    26761 accaatttaa aataccaatt tattaataat tataggacaa tgtgagcaaa tatagtcttt
    26821 tgagaagaac agagaaacga aggacctaaa ctaaacatat cgcaaattga agggtgataa
    26881 gtatataata ccgaccttga ttatttatag aaatccagga atagacatgc gcgcatctca
    26941 atggtttttg gtcactcaaa aagaaactcc caacgatgca gaaatagcat cccatcaatt
    27001 aatgctaaga tcaggaatga ttcgtaagct tggctcagga ctttatacct ggatgccact
    27061 tggccttaga gtgcttcgta aagttgaaaa tatagttcgg gaagaaatga acaaaactca
    27121 tgccatggaa ctgcttatgc catcagtaca acctgctgaa ttatggcaag agacgggacg
    27181 ttgggaaacc tttggtggcc aattattaac tatgaaagac agtaatcaaa gagagtactg
    27241 ctttgggcct acccatgaag aggtcattac cgatatcatg cgtaatgaat tgcaatcgta
    27301 taaacaattg ccagtcaatt tttatcaaat acaaaccaag tttcgcgatg aaatcagacc
    27361 acggtttggg gtaatgcgag cccgtgagtt tattatgaaa gatgcttact ccttccacct
    27421 cagtattgaa agcctgcaag aaacttataa ggatatgtat caagcctatt gcaggatttt
    27481 tgatagaatg ggactcaaat atcgagcagt tgaagctgat acgggagcca ttggcggttc
    27541 agcgtcccat gagtttcagg ttttggctga atcgggtgaa gatctgattt tttatagtga
    27601 cgccagcgac tatgctgcaa atattgaaca agcgaccagc ttaaaaccac ccaaagcgaa
    27661 tcaagcatgc aatgaaacaa tcacattggt cgatactcca aatcaaaaaa caattgatga
    27721 agtagccagc ttccttggta ttgccagtaa tcaaaccatc aaaaccttga ttgtcaaagg
    27781 caaggaacat cctatggtgg ctctggtctt aagaggagac gatgagctca atgaagtcaa
    27841 agcaaccaaa catcccttag ttcactctcc cctgtccttt attgatgaag aactgatttt
    27901 aaaaacgtta aaaacccctc taggctccat agggccaatc cagttaaaca tccctgtcat
    27961 tgttgatcat catgccttag ccatgccttc ctttgtctgt ggcgccaatc aggcagataa
    28021 acactttatc aatgctgcat gggagcgcga tgcaaaatac gatgatgcct atgatttacg
    28081 taatgttaaa gaaggtgatc caagccctga tggaagagga atgcttcact gctgtcgtgg
    28141 aatagaggta ggacacgttt tccaattggg tgataaatac gccaaagcca tgaatgcgtc
    28201 tgttatcaat gagcaaggac aattacaaac catgatcatg ggctgctatg gattagggat
    28261 cactcgtgta gtcgctgccg cgattgagca gcatcatgac gaacacggaa tcatctggcc
    28321 tcaagcttta gcacctttcc aagtcaatat tattcctctt aatggcgcga gatcacaaac
    28381 cgttaaagaa caggcagaat cactttatca gcaactcaaa tctcatggca tcgatgtatt
    28441 actcgatgat cgtaatgaaa gagcgggtgt attatttgct gataatgact tgatagggat
    28501 tcctcaccga ttagtggtca gtgagagaaa tctggaacaa ggctgtattg agtacaaatc
    28561 ccgaacttca agcgaaactc aactgatcaa tctggataag gtggttaatt ttattattga
    28621 gttaattaat aaataagatt ttctacccca ttgacaccgc gttatctgca ttccggcaat
    28681 gctgttcgga ttaaaaataa cccgaacggc attaaagaga agccaataat acaggactta
    28741 tatttaaaga atcacaaata aagttctttg cccaggttca ccatatctcg tttggtattc
    28801 attaatgtga gttatggata agatcagttg tcacctcgaa tcgttcgggc tgaggaggca
    28861 tttatgccgt ctcgaagcct tgtacgggat ttaatgttgt gcgccaaggc ttcgagacga
    28921 tgcttatgca tctcctcagc ccgaacgaac cttggatatc aactggtttt ataatcctta
    28981 tccatcactg acgttaaata ttaaaaacca ttggaggttg cattacagaa tccgtctgaa
    29041 actcctcttc aagattggaa aacgccccct caccacaaga aagtatcctg tttttagatt
    29101 ctatctgaac ctcatttact gggactattg gaggcgcggt tttaaaacgt tttagtttag
    29161 cctcaaaatc ataaaaatca ttgaggttat caaaacgctt gataattaca ttggttttcc
    29221 tttcttcttc aagaatctta tcgcttaaaa attgaatttt tttgttacca gagtccatct
    29281 gttgtgccag attgctttgg attaaaacca tttcattaac caacgcatca tattttgtca
    29341 ttattttttt agttaacaac gattgcaaaa tgctatctga gcgtccttgt aattcattaa
    29401 attgatacct ccaatcatcc tctgtctctt cctgtctgaa catgccggga atcaaaccaa
    29461 caatcctgcg agtcactgta gaaaaccatg atgtggactg ttcttcaccg accttatgca
    29521 ttttattata taattctgta agctcggctt ttaactgctc atcctttatc ttatcgattt
    29581 gctccatggt catcgtattt ctatccatgc tttttaacgt atttctgtca agaaaatgat
    29641 tttgcatgtt ttcaggaaat tgactgagat tttcaggtat ttgaccatta atccactcct
    29701 ttagtgttaa aatctccttc accttgcttc ggcattgctc tggaaacaac tctacttcgg
    29761 gattgccctt gatgcgatct ttcaaaaaag gtaagtgttt ggcatggatt ctattcagat
    29821 tcagcaactc taatctattt tgcttaagag aaaattctaa ctgacggtac gcctctttta
    29881 attcagttaa cctggattta tattgcataa acccttcaaa taatgcggat gtcccttctc
    29941 cttcaataat taactcaaca tactcattaa atcctgtata tccttttttt actttcacgt
    30001 taaaaacaga atatccttct attccctcca gctcctcctt aatttttcgc gctttaaaat
    30061 caaaatattt cgattcatct atgatatcct ggtgtttttc ctgaaaacta ttccaaagag
    30121 tacgaatcat ccctcgtaat tgcactagag gctcacgcaa tagggaatat gaaatggata
    30181 attcattgat ttttttacaa agaacataat acatttcatt caatgtataa accttgcgac
    30241 tctcattttg cttttgctga agttgccttt taacctcttg ccattcagaa tgagcagtta
    30301 agatctgact gttatttttt tcaaagtctt ctaaaacaga gctgaatttc tctcccaaca
    30361 aatagtattg agcttcttct ttgcactcct gaataaaatt aatatccagc ccaccctcat
    30421 ctctacccag aacagttaaa ctgtccatgg tatttaatgt ttgattaatc aaatcacgtg
    30481 tatttttatg atcaaatgaa aaaatccaaa catcctccaa ttgagcttga tgtttgcata
    30541 agtctctata taattttttg atagcaatct gtttggataa agaatctctt gcttgtttta
    30601 ctccgttggc caaaaccaat atttcattcg ccgctttttt tactgctgac cgctcaatcc
    30661 aataccaagg tcctctgttt tcaggcttag cccaagccaa tgtcttgtca agatgatgaa
    30721 tgatttgtgt agctgtacta tcaagatagg tttcttttaa aatcttaagt tctttatgtt
    30781 cttcaaaacc tgcttgctta tacactctca gcatatcact gataattggt tgcatgcgga
    30841 atttttcgct caatttctct ttgtctaacc atctgctttc ataatcaatt aataacaact
    30901 ccaaagcaag aaagttatcc gcattattag ctctctctat taggccttgt ttaacactgg
    30961 caggtaatga ggagttattg atttctttgc taatatccct ggcccacaat ttcagttggc
    31021 tgtgcacaaa agctaatcga tagggctcta aatcaccatc atggtagctt aacatcgcac
    31081 cagttacttc tactgctgac tcaaggtgac gatgactgcg ccttttctcc tgagttgctt
    31141 cttttttctt taaacgagct gataatttac tccactttat ttgttcactt aaatcgacct
    31201 cttccaaata tttacaacgc aatgcattga ctgagtcact ggagaatcta ttttctgttt
    31261 ttgctttcaa ctgatctcga tttaggttcc aaatgctgga aacagatttt tcataggctt
    31321 gaattgcctt ttccaattct ctggtttgta agcgctgaaa aacatcccta cggacatagg
    31381 ggtttggata cattttttta ggatcagaat cttccaaaca ctttgtccat tgcttcttta
    31441 gcgaatcaat caactcattt ctttgcgcaa ataattcatt atccagctgt ctccattctg
    31501 acttggggta aattaaatgc aaaaatgctt gccattcttc caattgattt aataccgctt
    31561 gttgaatatt agaaacagat tgtatgaaat actgtttaac tgcttcttct cttcgtcttt
    31621 cagccaaact ttgcgtattt aattcttcct gcaaattctt cctggcaggg aaaacccgat
    31681 accccacatc catatcgaac cggctgattt gcccaaccac ttcaccaacg gtttcctcta
    31741 tttcggttat cctgacgaca cgccccttcc ctttataata atcatacagt gaagaaaaat
    31801 gagagaaagt tctatcgtct aaattagaaa aatcaatatg attaagaata aggtagcttg
    31861 agacaggata gccgcatcta tccatagaca gttcctcatc ctctatcact gcgtgcgagc
    31921 gataagaagt caaatcagaa agtgttgagt aattaatacc tccaacctgg taagtaccag
    31981 actcagaatt cgcctctacc acagaggaag gtatacccaa acaggtaaag aaatctctat
    32041 cacccttctc aagataatcc tgagctatta atccgtgatt tgcaaaacac aggtcgacgg
    32101 taccaccatc agcaagcacc cattgcatgg cagcaagaat tgctgcaggt ggagcgctca
    32161 ggttttcatt acgactgact cccattaaca cattttgttc ttgatgatgt aaagacaata
    32221 aaagagaaat catttgtgta gtatcaaaga atatgccggt actgcgaaaa tactgctctc
    32281 gcatcactgc gagtaacttc agttgagttc gcagtttatc ctctttgcta atatcgagtc
    32341 ttctcatctc cataatgaaa aaatcagaca actcctgcaa cttattcctg cctaattggg
    32401 ttaaatcagt atacgtttca ttcgctattt ttagaggaaa atccctgcct attgcattga
    32461 taaatacaac ttgttgagcc aattcatatt gccctttaca cgataaaccc gatccatcaa
    32521 ccaaactttc aatcttggca ataacctctt gaatttgact tgtgtcaaat tgctcattaa
    32581 gaatttgttc tctcgactta atcagaatac cttcttgatt cactttggga gccctggatc
    32641 cggcaggatc tctatcaaat gaatcaatat ataattttaa ctctcttgag tattcacgca
    32701 atttctcaga aaaagcgtct aatgtgggat aagggggata atcaaataaa gaagaaatat
    32761 cttttagtaa aacactatgg atctcaaggc gagactgaac attgaataat aaatcaccat
    32821 ttacactctc aacatttctt ccaagtatag tcagtatttt tatccattgt tctaaggatt
    32881 tctcatcctt tttttcaagc ttatcgataa gacggcaata tctggtcaat tctgatttgt
    32941 cgtacttatt acttgattta atgagtaccg ttaataagga aaccaactca ggaaggtctg
    33001 tcgatttgga gtcaataatc tctgatgtgg attgaataaa ttgtatgagc agttcatgct
    33061 cgacatccaa gccagccttt tccactgcct caatcacagc atcaaataag gattgtgatt
    33121 tgttttcatc gattcccttc agaatattga actcaattaa ttccttcagg aatttgatct
    33181 ctttattgat gaattcgatt tgttcatcat ttcttgttaa acaaccacca aattccttta
    33241 cacctttgaa atttaattga tttcgagtga ttttcatacc ctgaattccc tgaaaaatag
    33301 aatgccctca ttatatataa gctgcataaa atgtagcaga taaatgtaaa agaaaatttg
    33361 aaatattact taatgtctgt attaggtgtt gcacctgtta caaataaata ttttaaaatc
    33421 tttatcagag aggaataatt tcgctttctc tccaatattg cttcgcaaaa ccccaataaa
    33481 taatttaaat catgcataaa gattcaatat tttttaatca cgttttcaat ttatcattaa
    33541 gcaaaaacta gcatttccca gatctttaac atgggtcgtt acgacccatg ttgtactgtt
    33601 tcacctgtta taaactacgc attgagcaag aaactttaac ttggaatcaa ataagaaacg
    33661 ctggctaata tagaaactgc ctgatttttt tcttctctat aatgggtagc agaacgccga
    33721 tcgggtgtaa aatttggaat agttaaatta ttggttccca aatcatggta aacaagctct
    33781 ccagaaaaaa tccaattctt gtgaacctga taattcgctc ctatgccaat attataacca
    33841 gtattgtaat tgtctgatgt tgcaccacct gtatagttgc ttacggttcc ggttactaaa
    33901 ttggttatag caccctctaa tacaaacctg tgcttgacat tcgcaaaact gacacccgct
    33961 tctacaaaag gaagaaaatt ggggaaaatc tcgtatccta aaaccgcatc aatattataa
    34021 attggcttga gagagcggac aaagttatca tggccactag ccagttcatt tggacctcta
    34081 gacgaagcaa agctgatttg gttattgttc tccattggag tgtagtatat tgaacctttc
    34141 cctcccaaat aggttttctg attaagcatc actccatatc ccgcttgtcc agaaaataga
    34201 acagcagaat tagttacttt atgaatgcct gttcgtataa ccccaaaacc aggacgaaca
    34261 attgaatact catccgtact aaaaattgta gtaaaaccag ttccaaggcc tgcgtagaat
    34321 ccgccaaacc ccgtttgctc aaccactggc cccatactcc ctgcatataa attcattgcg
    34381 gtcaataata atggggtaca gcaaatgaca cggtaattca tatcaactcc ctttgggtaa
    34441 tcaaaaaagt ggtatttaat catctcttca attgtttgta aaaatttaaa tgagattacc
    34501 caaatgaaaa cagggaatac caataactat gattcaatgt ccaatacagc atttaatagc
    34561 tgcctggtat aagggtgttc tggttgccga agtaccttct cgcacgctcc atattcaaca
    34621 gccaagccgt ctttcatgac taatacatca tcagcgatat aagagacgac ccccatgtta
    34681 tgggttataa acaaatagga aatacctctt tgctgttgca attcctttaa taaattaaga
    34741 atttgtgcct gtacagagac atcaagagca ctggtcggct catcgcagat taaaatatca
    34801 ggttctgtcg ccaaagctct cgcaatgcag atccgttgtc tttgtccacc ggaaaactga
    34861 tgagggtatc gatgcagact ggtaattggt aagttcactt gctcaattag ggatttttgt
    34921 tgtttcctga tgtaagaagg cttcattccc tgtgcatgca tcccttctgc gatgatttct
    34981 ccaactgtca tgcgtggatt catagaagaa aaaggatctt gaaaaataat ctgtactttt
    35041 tttctgtatc ttcttaaagc gcttcctgtc atagacagta catcactatc cttatatctg
    35101 attttccctc cggccgctgg caataacctc aacagagcac ggcttgcagt tgttttccca
    35161 cagcccgact ctccaaccaa agctaatgtt ttacctctgg agagagtgaa cgacaaacca
    35221 tcaacggctt tgaaaatggt tttcttacga ctcaaaaatc ctttcttatg gataaaatga
    35281 actgataaat catctactgt gaagacgatc tcttcttttt ctacggaagg aacccaactg
    35341 attttatttt tatccagtaa tggcaattct ttgatatcag gatacaaatg acatctcaag
    35401 atacgctgct caacttcttg taattgaggc tcctcatgat gacaacgttc aaaagcataa
    35461 atacagcgtg tatggaaacg acaccctgaa ggcatagact ccaaagaagg taccgtgcct
    35521 gcaatgatag acagtttttc atttcttttg gcaaatgaag gtaaggatgt caaaagttgc
    35581 tggacatagg gatgactcac ttttgaaaaa aaatctttta ctgaggcctg ttccactacc
    35641 tgccctgcgt acatcacaca aattctatca gccatggttt tagccacgcc cagatcatga
    35701 gtaattaata acaaactcat ctgatgtttc ttttgtattt tttttagcaa ggccaatatt
    35761 tgagcctgga tagtcacatc caaagctgta gtaggttcat cggcaataag gatatctggc
    35821 tcgcaagcca acgccatagc aataaccacc ctctgctttt gaccaccaga caattgatgc
    35881 gggtattgat aaattctcat ttgtggttga ggcatttcta cctcattcaa taacaatatt
    35941 actctttctt ttatttgctc ttttgtcaaa gaccgatgcc tttttaaaac ctcagctatc
    36001 tgctctccaa tcgttaatac gggatttaaa gccgtcattg gctcttgaaa aaccatcgca
    36061 agcctgcgcc ctcttaactc cctcatcatt ttctcgggta aatttaagat gtcttcaccg
    36121 tgaatattga tttggctgtc aatcccatag actccagctc taggcagcaa cctcatcata
    36181 gccaatgaag ttaaggattt accacatcct gactctccca gtaaaactaa agtctcccct
    36241 ggatttagac taaaacttaa tttatccaaa gccagaactg ttttatcagg gcttttaaaa
    36301 gcaactgaca attgattaat tttagcggtg ttttgcatgg aatttgccac aaataatgat
    36361 aactacactc taacaagaga ggggtgatag tagcaataaa aataccatac cactctgagc
    36421 aaaagaatgt ttatgataga ataagcacaa ttaatatttg agtgaatacc catgtcagaa
    36481 aactacactg cggaagcgat tgaagtctta agtggtcttg agcctgttca acgacgtccc
    36541 ggtatgtata ccgacacgac acgccccaac catttggctc aggaagtgat tgataatagc
    36601 gtcgatgaag tacttgctgg atttgccagc cgtattattg ttactttaca tgaagacggt
    36661 tctattgaag tcgaggatga tggccgaggt atgccagtcg atttgcatcc tcaactgggc
    36721 ttaagtggtg tcgaggtcat tatgactcga cttcatgccg gaggtaagtt ttcagataaa
    36781 aattacagct tttctggcgg cttgcatggc gttggggtgt ccgtggtaaa tgccttgtca
    36841 gaacgactgg acgtcaccat aaagcgcaat gggatcattt atcaaatgac ctttgctaat
    36901 ggcgacaaat tatgtgaatt aaatgaaacc ggggttacaa aaaaaagaga gaccgggact
    36961 gttatacgtt tttggcctaa tggtaaatat tttgacacta caaaaatttc tgtaaaacac
    37021 ctgactcatg tattaagagc aaaagcggta ttgtgtagtg gcttgtccat gacctttatc
    37081 aataaaatca ctaatgaaga aatgaactgg tgttatgagc aaggtttaac tgattattta
    37141 cagcaaacct tgcccgaaga ctatttgcct gaagaacctt ttgccggagc attcaaaagt
    37201 gatgaagcaa ctgttgattg ggctttggcc tggtcagact ctgccaatgg caatttaaat
    37261 gaaagttatg tgaatttaat cccgacaata caaggtggca cgcacgtcaa tggattgcgt
    37321 tcgggattat ttgaagccat gtctgaattt tgtgagctga gaaaccttct tcctcgtgga
    37381 gtaaaattga cagcggatga tctatgggaa tcatgccagt atgttttatc aatcaaaatg
    37441 aaagagccgc aatttgcggg gcaaaccaaa gagcgcttga gttcacgcca aacctcagga
    37501 ctagtcagta atgtcataaa agacgctttt gcaatttggt tgaatcagca tcgcaaccag
    37561 ggtgaagcca tagcagctct cgcaatagaa cgcgcacaga aacggctaaa gcaagccaaa
    37621 caagtggcac gcaaacgtgt tagccaaggg cctgctttac ctggaaaatt agccgactgc
    37681 ttgcaaacgg acttaagtca agcggaacta ttcctggttg aaggagattc ggcaggaggg
    37741 tcggccaaac aagcccgcaa taaagattat caagccattt tacccttacg tgggaaaata
    37801 cttaattcat gggaagtcga ttcttcacaa gttctggcat cccaggaaat tcatgatata
    37861 tctgttgcta taggcgtaga tcctggctcc agcgatctcg agggtcttcg ctatggaaaa
    37921 atctgtattc tcgctgatgc agactctgat ggagctcata ttgccacact aatatgtgcc
    37981 ttgtttttaa gacacttcaa acctctggtt aaagcaggcc atgtttttgt agccatgccc
    38041 ccgctctatc gaatcgatgc aggtaagctg attcactatg ccctggatga tgaggaaaaa
    38101 aatagaatcg ttcaacatct cagtcaaacc acaaaatcaa aagtgaatgt gcagcgattc
    38161 aaaggattag gtgaaatgaa tcccattcag cttagagaaa ccacaatgga ccccaataca
    38221 aggcgtctgg tacagcttac tcttgatgat gaagagcaga cggaagccat tatggatatg
    38281 atgctcaaca aaaaaagggc cgccgacaga aaactctggc tagaaacaaa aggaaacctt
    38341 gctgaaattt gagctaaatg tgacatgact tccttgatgt gactaaaaag tggcggttaa
    38401 aaagtcactt tctctaaaaa ggtttaaaaa tttcatagcc agtcaggaaa acaacgttga
    38461 ctagccgaag tgaaaaagac agcacttgaa caattgcctc tgtctagcca ttaccatcag
    38521 cttttctatc ttaaccttgt tcatgtagat agccggcatc aagataaagc aaggatacaa
    38581 ttgaatttga tagcttacct ttttacccta ccatttcagc aaggggataa tattagaata
    38641 atcatcagtc cataaaaact gatcatcatc tgctacaaat ttccagtttg ttcctttcat
    38701 gacttgaaat gccaaggatt gatttgaggt caacatagcc cactctgaat taaattgccc
    38761 caacttctta tctcccttat gcgctaaaaa taaagttatc atattcaaag ctcgcccaat
    38821 tgcattaatt accgggagca tttgcagatg tctgttactt agattaacca aaataactcc
    38881 atcttcgaca attttcttct tatataaggt gaaagcttca agtgtcatca aatggacagg
    38941 aatagcatct gaattaaacg catccaaaat taacaaatcc tgtgaagcat ctggcatatt
    39001 aaccaaggcc aatcgtccat cctgtttaat aatctcagct gaaggcgagc agtctcgtat
    39061 gtatgtgaat aatttattat ttttagctaa atcaattact tgttgatcaa tctcaataac
    39121 ctttatggaa tcagttgccc gaaattggca cagtagtgtt cctgcaccca accccataat
    39181 cgtcgcagaa agcggatgaa attccttctt caaaatatca actattggag ctatggcccc
    39241 ataataggag cttcttccat aacttgcttt ttccccattc attacctgca tcccatgaag
    39301 ggttgattgg cttattaatg catgcacatc atcctttttg aagacttgct tgacgccgta
    39361 aaaatttctt tccttgagta aaatctgttt gtcttgcaac ggaggaaagt aaaggaaaac
    39421 aaataaaatg agcatggata gtacaagatt gattttatta cgatgccaaa tcacaataat
    39481 tccaagtgcc aaaatagcaa gaatgtgaaa agtggtagta cttttaaacc agggaacggt
    39541 aggaatgaaa taatgaagcg acagcaaacc caaaacaact aatggcaccc accatcctcg
    39601 gttagttttc ggcagcggaa gaacaaataa acttaataaa atggctaacg ggtattcata
    39661 aacataatta aaccaatttg gcgctaaaac tccattaaaa atccctgcta aaactcctcc
    39721 gatagacaaa caaaaataaa acaaagtgag tttatgtggc ttgggtcttc gtgcaaataa
    39781 ctgaccatga cacagtaaag ccaaaataaa aaaacttaac agattaaata acaccaattg
    39841 ccacactctg atttgactaa ctccaaaaat gaacccaaga attgtaaaaa ccaggaaaaa
    39901 caaacaatta cgagaaatcc atttttgtga aataaagggg gtcgctgtga aggtaaatac
    39961 aaatgacaaa aggtaaagcg ctaaaggcaa aacccaaaat aagggagttg cggcaacatc
    40021 agttgtgata tataaagtaa ctcccagcat caaactgcaa gggacaaaac tcaaaaaaat
    40081 ccaataggct atctctcgcc aaggcaaaaa atccacctct tttttttcag ttaacattaa
    40141 gggttggtat tttgtaaaaa acaagactgt taccaacagt agcgaataaa tagcaaatcc
    40201 aaagttccag aaataaaatt gatgagtcaa accaataaat cgctcgataa cccatggata
    40261 aataagcaag gcacccaagc ttccaagatt actggatatg tacaaataat aagggtctga
    40321 ggcccctttg ctttttgtct ggctataggc aaattgtaat aaaggagcag aagcaccaat
    40381 cactaacaaa ggcaaaccca gttgaattaa caaattatgc agaatcaccc attcaggttg
    40441 tccatccctg gctgcaggat gaaacagcaa tggaaatgct gtaaaactta aaacaaccaa
    40501 aaccatatgg atcaaacgcc aaacagcagg tttcttaatc tgacttaaaa gccatacgta
    40561 gccatatgca atgagcagaa taaattgaaa aaaaagcacg caaacagtcc acacacctgg
    40621 tgtcccccca taaattggaa gtattgcttt tgcaaccatg ggttgaatac tgaataggag
    40681 aatggaactt ataaaaagac tgacaggaaa taaaaatcga aacacaaagc aatccttagc
    40741 caatggtgct gccaaatgaa tgaatcgatc attttacgac tacactctcg ttcatacaag
    40801 gatgaattat tactctgcaa aacaatttat aaacataatt gatagggatg tttcacaaac
    40861 acatattatt ccatattgac tgatggaaaa atccatttat ggtttttgaa gcttttgtaa
    40921 acatcccatt acttgaagat aggcactgat tccatatcaa ctgatacata aaatgaatac
    40981 tttatttctt ttccttggag gctgataaca tccaatgagc tttttcatga gccgcaactc
    41041 gatcgcttag aatagtcaca gtaccttcgt cttcattttc ctgggcaatt ttaatcgcct
    41101 gatttaaatc ttttactatc atcatattat cttttgccaa ctcactgacc atctcattag
    41161 cactcaatcc ggaatcacca tctttaatcg ttttcaattg attaaactca gcaaaggtag
    41221 caggagcctt atgacccatg attcgaattc gctctgcaat ttgatccact gcttccgcca
    41281 gttctttata ttgcatctca aataattcat gcaaactttt aaattgaggt ccagtaacat
    41341 gccaatggta attttgtgtt ttcaggtaca acgcataagt atcagcaaga gcgacttcca
    41401 gctttttaat tactttactc attattcttt ccttgttatt tattcacgtt attaaggata
    41461 gttcaaaaca gtcaaagaac aaaatagaca ttaataaaaa acccgccttt acagcgggtt
    41521 ttttattgga taaccgggag aaaattacat cattccgccc atgccaccca ttccgcccat
    41581 gccgcccata tcgccggcac caacaccttc ttctttctta ggcagatcag caaccataca
    41641 ttcagtagtc aacatcaaac tggctacaga agctgcattt tgcagagcca tacgggttac
    41701 tttagttgga tcaagaatac ccatctcaac catatcaccg tattcaccag ttgcagcgtt
    41761 gaaaccgtag ttgtctttgt gctcagctac cttgtttact acaacagaag cttcatatcc
    41821 tgcgttagta acaatctgac gcattggaga ttcaatagcg cgacgtaaaa tattgatacc
    41881 catattttga tcgtcattat cgcctttcaa tgaatcaaga gctttctgag cacgaatcaa
    41941 ggcaacgcca ccaccggcaa cgataccttc ttctactgca gcgcgagtag catgaagagc
    42001 atcttcaaca cgagctttct tctctttcat ttcaacttct gtggcagcgc caactttgat
    42061 aacagcaaca ccaccagcta acttagcaac gcgctcttgt aatttttctc tatcgtaatc
    42121 agaagtggtt tcttccattt gtgcacgaat ttgagcaata cgagcattaa tttcagttgc
    42181 ctttccttca ccatcaatga tagtagtgtt ttctttggta acaacgattc gcttagcact
    42241 accaagatct tccagagtag caccttccag gctcttgcca atttcttcag aaatcacttg
    42301 acccttagtc aaaatagcaa tgtcttgcag catcgctttg cggcgatcac caaaaccagg
    42361 cgctttgaca gcacatattt ttacaatacc gcgcatgttg ttaactacca gagtagctaa
    42421 agcttcgcct tcaacatctt ctgcgatgat caataaagga cggccagatt ttgcaacacc
    42481 ttccaatacg gacaacattt cacgaatact ggaaactttt ttgtcaacca ataaaatgaa
    42541 tggatgctca agttcacagc tcatgttttg ctggttgttg atgaagtatg gagaaatgta
    42601 accgcgatca aattgcatac cttcaacaac agaaagctca ttttccaatc cattaccatc
    42661 ttcaacggta ataacaccct ctttaccaac tttttccatt gcttcagcaa tgatagcacc
    42721 aatcgcttca tcggaattag cagaaatagt tccaacttga gcaatagctt tgctgtcttt
    42781 gcatggctta gacatagctt gtaatttttt ggtaactgct aatactgctt tatcaatacc
    42841 gcgtttgaga tccattggat tcataccagc agcaactgct ttgtgacctt caacaagaat
    42901 agaacgagcc aatactgttg cagtagtagt accatcacca gcagtatcag aagttttaga
    42961 agccacttct ttaaccattt gagcgcccat gttcatgaaa cgatgctcaa attcaatttc
    43021 tttggcaaca gacacaccgt ctttagttac agtaggagcg ccataagatt tttccaatac
    43081 aacattacgg ccacgtggac ccatagtaac ttgaaccgca tctgctaatg cattaacacc
    43141 agcaagcatt tgtaggcgag cgtcatcacc aaaacgtaat tctttagcca ttatcattgt
    43201 ctcctttaaa caattagatt acttctcgat tacacccatg atgtcgtctt cgcgcatcac
    43261 aactaattct tttccatcaa ctttaacttc agtaccagag tacttgccaa acaataccac
    43321 atcacctact ttaactgcta aagcacgaac atcaccgttt tccaatactt tgccagcgcc
    43381 gacagcaatg atctcaccac gcataggttt ctcggtagca ctatctggaa taacaatacc
    43441 accagcagtg gtacgctctt cttccatacg acgaacaaca acgcgatcgt gtaaaggacg
    43501 aattttcata cttttctctc ctgattaaat taaactttaa atcatttaag tattgctttc
    43561 cttagggcaa acaataccga tgacagcgat atggggacaa taaacaaact ttcaagggta
    43621 aaatcaaaaa aaattttgga tttgggatta cagagtctac aataatgttt attagtaact
    43681 gtaaaagata tcgaatgaaa aagtggctct tattttccct attattttta acttcccttt
    43741 gtgttcatgc tgagccctta ccagcttctg aagtatttaa ggttagtgta aaaaaaatag
    43801 accccaatac ctttgccata caatggaata tcttgcccaa gtatttttta tatagcgacc
    43861 gtattcaatt aaattccgat aatgatgaca ttgcacagct tggaactctt cgcttcccca
    43921 cccccttaac caaaacggat aaacaagggc gaacctttaa agtttaccgc aatcagctta
    43981 cccttccagt gggggtcctg ggcattaccc caggagaaac aatagtcaat ttgcgttttc
    44041 aaggatgtgc tgacgatggt ttttgttatc cccccgaagt aaaacaaatc aaactggcaa
    44101 ttgacgataa actggctctt tctcaagttg atttggaaac attccatgca cctgaagcaa
    44161 aacctcttga aaaacctgaa caagacatcg ctgatatttt tgcaaatcat aattggataa
    44221 tgattctgct tatattttac gggtttggcc tgctcctgtc cttcacgcct tgtatactgc
    44281 ctatggttcc tgtcctttca ggtattattg ttggccatgg gagaacggca acaacgaaaa
    44341 aagcgttttt cctgtcatta agctacgttc ttagcatgtc tgtcacttat gctattgtgg
    44401 gtgctgtcgt tgccttgctt ggagccaatt tacaaatcag tatgcagtca ccttgggcta
    44461 tcagtctatt cagtctgata ttcgttttgc tggccttgtc catgtttggc ttttatgaat
    44521 tcaaactacc agatgcatgg caaagcaaaa ttgtaggttc aagtcgtgaa caacgtggcg
    44581 gacactatct tggggcagcc atcatgggat gtttgtccac actcattctg tcaccatgcg
    44641 ttacagcacc actaattgga gtattaacct acatagcaca aaccggaaac gtattactcg
    44701 gcagtgttac tctatttaca ttaagcctag gcatgggtac tccattactg ctgataggaa
    44761 cttctgctgg taaatggcta cccgaaacag gaagctggat gaatgctgtt aaagctttct
    44821 ttggcatatt acttcttgct gttgccatct atctgatggc acgcatttta ccagcaggtc
    44881 tggtcatggg gctatgggct tgtctcctga ttttttccgg catttactca ggtgccttaa
    44941 ccaaatcaaa tacaaaccag gaaaagctct gccaaggcat tggtatcatt ttattaacct
    45001 atggattgct gattctcatt ggggctagca tgggctcttc caaccctttg cagcctttag
    45061 ccaacttaca agcagcccct actgtaagca atgcttttga atcagctaaa gcccaatctg
    45121 ttaaaagtgt tgaactggct ataaaacaag cgtttggaaa accagtgatg cttgattttt
    45181 atgctgactg gtgtgcatca tgtaaagtga tggaaaacac cacgttcaaa gatcctcgtg
    45241 tacaaaaagc tttgagccat tttatcgtta tcaaagtgga tgtaactgcc aataataaaa
    45301 atgacagagc attaatgcag catttccgtg tagtagcccc cccgactttt atctttttta
    45361 atgctgatgg cgtacaactc aataatttga aaagagttgg tgaattgaac gccgatgaat
    45421 tcatgcaaac aataaaaagc attgatgaga actaaaataa ctacgggcac ttttgtatga
    45481 aatcagtcga cactcgaacg attaagcagt ataattagct catttttttt acagatagaa
    45541 gtttatgaat cataaagtag gatttgtaag tttaggatgc cccaaagcac tggtcgattc
    45601 agagcgaatc attactcagc ttaaagcaca aggctatgaa ttggttccca cttatgagga
    45661 tgctggagtc gtcgtaatta atacgtgcgg cttcattgac agcgctgtcc aggaatcact
    45721 ggatacaatt aaagaagcga tggctgaaaa cgggcgagta atcgttacag gttgtttagg
    45781 agctaaagcg gacgtaatta aaaatgcctg tcctgatgtc ctgcatatca gtggtgctca
    45841 tgcctacgaa gaagtagtca atgcggtaca ccaacacttg ccgcctcctg cagacccatt
    45901 tacacaactg attcccccac agggaataaa acttacacct cggcattatg cttatttaaa
    45961 aatatcagaa ggatgcaacc aaaaatgcac tttttgcatt atccctacga tgagaggaaa
    46021 attgcaaagc tatccaatgg cccaaattct tactgaagct aagaaattaa aacaagccgg
    46081 agttaaagaa ttgctggtta tctcacaaga caccagcgct tatggcgtgg atacacgtta
    46141 tcagcaggtc gaatggcaag gaaaaacagt caatacccgt ttttacgatc tatgcgaaca
    46201 attaggcgaa ttaggaattt gggtgagact ccattatgtc tatccctatc cccatgttga
    46261 tgatattgtt cctctaatgc gcgatggcct gattttgcct tacctggata taccattgca
    46321 acatgccaat tcacgcatat taaaagcaat gaaaagaccc gccagcagtg aaaatacctt
    46381 attgcggatt gcttcatgga gagaaatttg ccctgacatt acgttacgct ccacattcat
    46441 tgtaggtttc cctggtgaaa cagaagagga gttttccgaa ttactagcct tccttaaaga
    46501 ggcacaacta gacagagtcg gttgtttcaa atactcccct gttgaaggag ccaaagcaaa
    46561 tgatttggat aatcctgtat cggaggatat taaagaagag cgttatcatc gttttatgca
    46621 agtacaagca gaaattagcc gtaataaatt aaaaaataaa attggcagta ctcaaactgt
    46681 tttaattgat gagataaccg aagatcaaat catagctcgt agtaaaagcg atgctcctga
    46741 aatcgatggt cttgtttatt tgccaaaaat atccgggatt actgttgggt ctttcgcaga
    46801 agcaatgata accgacagtg acgactatga tttgtatggt gatttggaat actctcttgc
    46861 atgaaagaaa tggcattaaa acagtacttt ttctgctcac cttcattggc aagagagaga
    46921 atatttattg tatctagcgt acaaaatgcc ttttaaatcg ccatgtcgct aaagaaatca
    46981 tggtaaggca atacgcaagt agtggccata aattagccca aatttcacca aaattatttc
    47041 cttttagcat aatgccaaac ataatcttaa aaaaatgcgt caatggcaaa aataatccta
    47101 aatattgcgc ccattcaggc attccaaata tcggaaaagt aaatccagtt aacagaatag
    47161 aaaaagcaat gaaaacattg atcacttgca ctgcttcaaa ctgagatgta caaaaagtgg
    47221 ctatagttaa acccaacgat aattccgcca caatataagg taaggtgcat aaatataaaa
    47281 gaagaggggc gccaataaac ggaacgtgga ataagtagta agacaagata agacccaaac
    47341 ttaattgtaa ataaccaatg aggatataag aaaaaatttc gcctaataaa acttctgatg
    47401 ggcgggtcgg tgaggctaat aaatattcaa tcgtaccacc ctgaacgtct ctaaatgcca
    47461 caaccacggt tatcagcagc atcgttaaca tgagcaccaa gcctatcatc ccgggaacca
    47521 cataaaattg agttacgcga tccggatcat ataatcggtg gtttatgatt tgaaaatcag
    47581 gcctctgcaa gggaatatta ataccctttt gacccatttt ctctataaat gatgatttca
    47641 atcccgccaa agcaatgata gctctacctg aagcaatagc atctatactg ccatcttcca
    47701 ataaaagagc tggattttgg tttcgacgaa ttgctttggt aaagttcaca ggaatagtca
    47761 ataccaggat agccttgcct gtcttcaata aatgatgggc tgattctgaa tcgttcgtcg
    47821 aagccacaaa tgaaaaatac ccggtatttt gtatctctcg aaccagttca cgtgttacat
    47881 cagtattatc caaattgatt aacaccgtag gaactttttg aggataggtg tttatggcat
    47941 acccggctat acaaactaaa atcaaaggta agattgccat aatcacgata gtagcaggat
    48001 cacgtttcat caataggaat tctttgcgga tgatagccca caaacgttgc attgaaaaat
    48061 gactcttcac tttggattcc cttctttcat aattttataa atgaaagcgt cttctaaagt
    48121 agtatctgtt tttttgattt cataattgtt caacacgaaa ggatccaata cttgatgtat
    48181 atcatcaact attgagctga tgcgcatttc attacccttt tcaatcattt gaatagcggg
    48241 gttatttact aatatgtcct ttaatctgga gaaactctca cctttaacaa cccaggagtg
    48301 caaacctgtc gattgaataa tttcaatcac tgagccacgt gctatgattt ctccggcgga
    48361 catataaacc aattgatgac atctttctgc ctcatccata tgctgtgtgc tgagtaaaac
    48421 agtaactcct ttggatacta tactttgtat atgagcccaa attaacaaac gcgattgagg
    48481 atcaatgcct gaagttggct cgtctaacaa aagtatacga ggcttatgca acaaagcagc
    48541 cgctaaagcc acccgctgct tccaacctcc tgataaagta gcagtaatcc gtttttggtt
    48601 gtcacttaag gccagtaatt caatgatttc cttcagtctc tctttcctgt tttttaattg
    48661 gtatattctt gcgataaaat ccagattttc ataaacagtc agattttgat agaggcaaaa
    48721 attttgtggc atatagccaa tttgagcctg tatcaacttg gtttgggtca ttaaatcgag
    48781 attcagacat ctaccctggc ctttatcggg aattattaat ccacttaata atcgtaaact
    48841 ggtcgtttta ccactaccgt ttggccctaa aaaaccaaaa attgttcctt cctcgatttg
    48901 caaattcaag ttcactactg cgttgatacc attaaatgat tttgctaatc cggacacatc
    48961 gataataatt tgctttgagc tccatttact cataattgac atccacaggc tgacctattt
    49021 ttaataaatc ccttaatccc tgagatagat ccgcctttac tttgtatact aatttataac
    49081 gatttttttc actaaaaata gcatcaggag tatattcagc ctgattggat ataaagctta
    49141 ttttgcctga atattttttt tcattaagca aaattcgaat ttttttacct aatttaattt
    49201 tttccaaatg gctttcggga acataaaaca ggattttcat ttgagaaggc agagagagac
    49261 tcgcaatagg gaaattagga ggtacgtact catcctttaa ataataacgt tcagtcataa
    49321 cagagtctgc gggcgctttg attgaattta ccccatcaag ggttacaatt ttatcctctt
    49381 tatgcagtaa ttgaccttca tcagcatacc attcttttat gtatccacca tccgggcttg
    49441 acacaataaa taactgagat tcaacatagc ttgtcgtttg atatggtgat ttttcttttt
    49501 tacaaccttg caatgtgcaa caaactaata caaccaggat tgcaatccct attctcatac
    49561 ctcttatcct tttgtcatca gtattagtta tcctatcagc aaagcaataa cgattgggac
    49621 atccactatt gggtaaatct ctcatccagc caatcaaaaa taacttgctg aaactgacca
    49681 ttagcatctg cttcacaatg catacctgcc cccagtttgg catcaaacat cacataggtt
    49741 ttttcacaac gcaaagcttc atatagcgta ttcccacgat tggatatcct gtcgcttgga
    49801 ttatcgcaga ccagtgtggg acaagtgatc tcttgcaccc tatcaacaaa ggtgtatgca
    49861 gataatgcct gcaaatagtc aaaaggagta gccaaaccgt gtgtatacat tcttgatttg
    49921 aaataaaatt ctttcattgg attttctgag aacaattgat aaaaacaggc attaagttca
    49981 tcagcttttc cttcttttaa taacatcata aggtttggag gtagcatttt attgagtgag
    50041 cctgcaatat ccatctgtcc tggatcacaa attaatgcag ctaatcgttt ttcactgcag
    50101 gccgcccttg gtgcaagata accgccaaaa cttctgccaa tgagcacata actggaagtc
    50161 cccagttcac tttgttcatc taaccaatcc acaacctgag agacaacatg ttcgaaatca
    50221 gctctcatat acagcttttg tcgacgcaaa acatgccctt gtcccgggcc atcaaaaatc
    50281 aaaatatgat acccatgtaa aagcgcagca ggaacgagag cataattttc ttcaaaggta
    50341 ctgtcatatc ctccaggtac tatcaaagtt gctttgggag attgatcgct gaaaaataaa
    50401 taaccgctta atctttgatc atcatagggg atccatattt tttgagatcc aggatgcaat
    50461 cgatgcattg ctcgttcaaa acaaaattgc aattgatcaa aacaaggaat cacacgcgga
    50521 tcatcaggat tggcacgtaa aaaaaactcg cttgcccggt aatattctgc agcacgcaga
    50581 taagcgtatg ctgctgttat aaaatgattg gcttcccaac tgatattggc tttggtttta
    50641 agtgtgtttg ccatctcata ccatgcctta taccaacttt ccccgtcttt atcagtgatt
    50701 ttggacaagg ttgcataaca ttcaccaaca tcagcacacc gcacataaca cttaccaaga
    50761 gttcttaaaa actgcccaaa aaactcatca ctgaaaatcc ctgttttttg atacatatca
    50821 taataattag catacataac agtaaaaaat tccctaaaaa ttacagagta actgaaagta
    50881 aaatggattg ccatgtgcca gagtatatta cgcgcaggcc tatgaattat cctaaatgat
    50941 attaggacag acttacgaaa accccaaggt caaggcaaaa aatgttttta atgagggagt
    51001 ttagataaac taaatgaccg aattaaaaac attttttaac gacgagattg ggggggtgca
    51061 agtctatagg attcattttc aggggagtgc tgtctaaaca caatctaata ccgataaaga
    51121 tctttgcaaa ctgaagacac tgcgttttgc atttcgtcat cattaataac atctggctgt
    51181 acaattttct gatgcatcaa ataatgaaaa tactgataag ctttctttaa agttgtgaat
    51241 tgagatttcg ttaatacatt cgataaaaac aaatgcttaa gttggcttag tgtattggta
    51301 taacttgaaa atttgggatc cctaagattc aatataagaa attgaaccaa aaattccaaa
    51361 tccaataatc cgccacgagc tagctttact tcatctgact cttgaaattg atcgatttta
    51421 gatcgcatgg ttaatacgtc cctttgcaat aatttactat ctctctctag caacaggact
    51481 gattttttta gttgggaaaa ctgatttttg atttttgaat ttcccgataa tatcctcgcc
    51541 tttaacaaag cttgatgttc ccatgtccaa gcttgtgatt tttgatactc aacaaaagca
    51601 tccacatgac tgactaacaa gcctgcagca ccggagggtc ttaaacgagt atcaacgtga
    51661 tacaaaaccc ccatttgagt acgagtagtc agcatatgta atattttttg tgttaatcgg
    51721 gtcactaaag cttcttcaga tggtttggca gaatgtaaaa aaaccaaatc gagatcagaa
    51781 gcataactca tctctcgact gcccagtttt ccataagcaa taatagcaaa tcgtgattga
    51841 atttgagcca tttcagggtg acggccacat aactgtttgc ttgcaataat taaaacttga
    51901 ctgacaatga cttgcgccac atcagacaga aatttcccta tgcgcacagc gtcacataat
    51961 ccatacaatt ccgctctggc agccaccatc caattcgtta atttaaactg acgcaggatt
    52021 tcctcatgca cttccacatc attagtatgc tctaattttt caactattgt ctgttccatt
    52081 tgggataatg aatcaggatg ccaatccttt tcctgctcca acaagatttc caacaaaaaa
    52141 ggttggctaa caaacaggga agaaataaaa ggactattca caaaccaaaa taataattct
    52201 cttagcgcat gggggttttc agtgagtaaa gccaggtaag cacttcgccc cacaatattt
    52261 tcaagcaaat gaattacttg taataaaacc tcatccgttt tagggcagta agtcaattct
    52321 gtaagcaata aaaccataaa acgatcaagg cgaatgcgag cgccctgtga taatcttctg
    52381 caccgtggtc catgtcgaaa agcatggagc atttgataac aatgttgtgc attttcatac
    52441 cctaaactaa tcaataaatt cactgccata ttactttcaa catgaccttg ccaaaggcta
    52501 aataattgat tgtttaacaa tctcttttcg tcttcataat catcaacctt gccaagaatg
    52561 gaatgaaaga aattactaac aattcgttga tattgttgta atttattgaa aaattgttcc
    52621 caatcagtga atcccatggc ataaactatc tgcacttttt ttaactggtc ttcaggtaga
    52681 gaatgggttt gttgatcgtt caaactctgt agaatatttt ccaattttct caaaaaaaga
    52741 tacgcttgtt ttaaagcatc acacctgggt agtaattttt ccttttttat cacagccagc
    52801 gctaacatgg cattttgaac ttgcaattga ggtaatctgc ctccgcgaat taattgaata
    52861 ttctgaataa taaattctat ttccctaata ccaccccggc ctcgtttaat atcattcaat
    52921 aaggggttaa gttgcacctc acgctcgatc attgccttca tccctcgcag ggattctatc
    52981 actccaaagt ccacatacct cctgtaaaca aacgggatga ttaaacgctc aaaccatgga
    53041 aatggttcat ccaaagactc agcaataacc cgcgctttaa ccatggcata acgttcccaa
    53101 tcacgcccct gttcctggta ataggtttcc atagctgcaa aactggaaac caaaggacca
    53161 ctatctccat taggtcgtaa tcttaaatcc actctaaata caaaaccatc aggcgttaca
    53221 ctctgtaata aatgaacaaa tgtttgcacc atcttgctaa aataatgctg attggtaatc
    53281 tgctcctcac catcagtgct gccaacttca ttgaaggcga aaatcaaatc aatatcggac
    53341 gaataattaa gttctcttcc cccaagtttc cccatggcga gagcatacaa ttgaatctct
    53401 ttaccctcac tgtttctagg aaccccgtaa cgactcaaca aggtaaatcc aatatatttt
    53461 aaagtatgca agatgataga gtctgccaga tcagaccagg attgcataac ctcttgagta
    53521 gaagccaaat cggctatttc aagtaataac aaccgcaaaa aatgggtatg acgaaaccga
    53581 cgcaattcct gcatatactg ggcttgagat aattccaggc tgatttgctg aatggcataa
    53641 aaatagcttt cgcgagacca taaggaacag caggaatcac tttctaaaag agagaccaat
    53701 aatctaattt gtctgcaggc atagtcactt accaatatca acttttcggc actttctctc
    53761 aatggatggt gcaaaggagc aacgtacttt tcaatcaacc aggattttgt agaaatcaat
    53821 gtttcaatat cgctttgtgg agacataaaa tatactattc ttaatataaa aacctaaaca
    53881 tatcatagga ttaatcaatt aattttacat ttaatacttt aaaactccta ttggacgtta
    53941 tactcttata aaagactcta ataatatttt agtgaagata ccaatgaaat ttattcaggg
    54001 attattgctt tctttctttt ttctagtttc acctctccac gctcaaaacg agcctatacc
    54061 tttaatagta ggtgtaggag gcttttaccc cccctttatt atgcaaggaa ccaataatga
    54121 gctttttggc tatgatatcg ccaccatgac agaactgtgt aagataatga aacgccaatg
    54181 taattttcga gtcatgagat tcagacaatt aattcctgct ctgattaaca aacaaattga
    54241 tgcggcgatc agcgccatgt ctattacttc agaacgttca aacctggtta attttagcat
    54301 gccctattta ttgagttact ccaggtttct tacaaatgag gtacacaata ctcatcaacc
    54361 gtttaattta atgctcttaa aaggtaaacg aattggagtg gaacaaaata ctgtctttag
    54421 tcaggaaatc agaaggatgg ggataataga ccctgtcata gtggaatttg acagaactga
    54481 agaaatgctt gaggcgttga gcgcaaataa aattgacttt gttctgatgg ataatcctgc
    54541 cgctttatac tggtctgcta attcgtcggg gagacttctg ctaataggca gtcccatgtt
    54601 gattggttat ggtttgggaa ttgcagtcaa tcgggaaaac tccgtattac tcaaatctct
    54661 aaatgatgcc ttggtagaat accagcgttc tgacaaatac aaggaaaatt acaataaata
    54721 catgatgaat ttttaagagt gatatggtta caaaaattgc tcttaatcac tcatcaatag
    54781 ctgaataacc gttttaaggc gtaataagtc agcgccctga tatttaacta tcctgtaagg
    54841 gccggatctg agcccaggtt ggatgaaaat aatcatgaaa aagcacagca tagatgacta
    54901 tttgagcatt tcgaaggatt attttctttc aatataaacc caatacggcc attccaattg
    54961 aatggctcat ctctagcttt taatgcagag tttgaccttg agtaatgcag cgactaatca
    55021 cctgtctggt aacgtaatca ccctgactaa ctccatataa agaagtaaaa tgttttgcca
    55081 aaataactac ccaaccagat aaacggaaat tcatgcgttt ttcattttga tcaacgacaa
    55141 tctcgaaaag acctaatgaa tcactatcac gttcattagc atactcttcc attatgactc
    55201 gcgccgtttg caaatcttta tctctggtag gccaattttg actcataaga cactccctaa
    55261 aaacatgagc aagactgttg ttacttctac aacatggagg caattttcaa aattgcaagt
    55321 tgtgaaataa ataaatatta tctcataaag tgattaaata ctcaatattg cggttaattt
    55381 ttacactgaa ttaaaaatat ttatggttta aaatacccat tgaaaatagc tttctccaag
    55441 accattgcgt tcggaacttc tcaagagtac attaatatat cattaaatct ttgacatagt
    55501 tttttaattg ctcaacaaaa cttcaaatcg gataaaaaat atcatgcata aaaccataaa
    55561 tggtgctatg acaggtattg gcctaagagc gccacattat gcccaaatat tagagaaaaa
    55621 acctgatatt gattggctgg aagtacatac agaaaatttc ttggtcgagg gtgggccttt
    55681 actagagctt ctagattcta tcagtcaaca ttatccactg agctttcatg gagtcgggtt
    55741 atctttaggg tctgctgagg gagtctgtat agcgcattta aatcgcatta aaactctcat
    55801 tgaccacttt caaccagctt tgatctctga tcatttgtcc tggagtacca tcgggaacac
    55861 ctatttaccc gatttattac ctattcccta taatgaggaa agtttgacca ttattagcaa
    55921 taatattgat agagtacaaa attatctaaa gagaacctta ttaatagaaa acccctcttc
    55981 ctatttggaa taccagacct catcgtattc agagcctgat tttttagcgg aaataatcaa
    56041 gcgtactgaa gccaggctct tattggatat aaataatatc tatgtttcct gttctaataa
    56101 caaacgagat ccttatcaat atatccaaag aatcccgcat gaagcagtac aagaaattca
    56161 tttggcgggc cattccattg cttatcagga caatacgaat ttcttgctaa tagacacaca
    56221 taataattat gtgtgtcccg aggtatggga tttgtatcga tatgcaatac attatacagg
    56281 ccttgttccc actttgttag aatgggatac agaaattccc tcgttagata ctttattaaa
    56341 tgaagccagg aagtcagcct gttatgccta caattaaaca aatgcaggaa ttttttctga
    56401 acacagtatt aaatcaacaa gatattgcta tttttgagta tttttgccct gacgagatta
    56461 ctaatgaatt ccgtttcgac atttatcgta ataccatttt tcagaatcta cgccattccc
    56521 tggaaatgac ttttccagcc atttggaaat tggttggaaa agaatgtgcg gatggtttgg
    56581 ctttacactt tatccaggat aaaaataatt tgccaacaac ccattgtttg gatgattggg
    56641 gtgaaaaatt tcctctgttt ttaaaaaata atccaaccgt tagtcatctt ctttatttga
    56701 aagatatcgc agaaatagaa tggttaaagc atttaagcta ttgctcgcgg gattgtcttg
    56761 tttttgatcc ccagaaactg caaaaacacc taaatgatcg agtagaaaaa ttaattttgc
    56821 tatttaatcc ttccgtgttt ttatactcat cctcttatta cttaaaaaat atttttgatc
    56881 tgatagaaaa tcccacggaa acaggttcta ttgattttca acaaatacca agttatgcgg
    56941 tgatatctcg tcaaaaaaat caaacagtga ttcattggat tactcacgat ttgtttcact
    57001 ttttatgcca catcaaagca ggggcctcac ttgggttcgc atacgaaaat actctggaaa
    57061 ataatcccga ttttgatttg ctcactgcat tacaattttt actaagaaat gaattattgg
    57121 aagattcttt tattcattct aattatcatt tgactcacgc taacaactgt cctgaataac
    57181 cgtgaagaac aatatgggtt attttgccaa taccactaaa tccaaaggtt ctcccacacc
    57241 acctacattt cttgcattca attccttctt agttattttg tcctcataac cttgttttac
    57301 cgcttctaaa aaagtatccc gggtagtcaa agcctgggtt ttttcttcct tattatgttt
    57361 ttcaagcttt gcagctaaat tggcagcttc gccaattaca gtatattcca aacgattttc
    57421 ataaccaatg acaccaacta ctacttcacc tgaagcaaga ccaattccta caccgaccac
    57481 tatctgttct tttttacggc gattttcact ccatacgtct accgatgcga caatttcatc
    57541 cacagtcctt aatgcatctg ctgcataagt atttgaaggg gtcacagcac cgaaacttgc
    57601 caaaatacca tcccccatga atttatcaat gctgccatta ttcttttgaa taatcggaac
    57661 cagtaagcgt tgatattctg ctagcagtcc gattagttca ctcggggaca gtttcactga
    57721 tgctttggta aatcctcgca tatcaatgaa cataattgca gcctgacgaa gctcgccata
    57781 tccagcttgc aatgactttt cagatgttgt tattttttcg accacacttg tatcaaaaaa
    57841 acgtgataaa tcttttgcag cgctagtttg ttctactgct tgaaacagag tgtgtttcgc
    57901 acgcgtcaat accaaggcta tgatggtagt aaccagtaga acggacaata tgacatcaaa
    57961 tacagctccc agatagacac tccgagttga cgcgaatgtc acataatccc atgtaataac
    58021 attcataccc gaactggata aagtttgcca ggcaataatt ccccagccaa gagccgcagt
    58081 gaggccagag agaattaccc ataatggttc aaatcgaagc gcacgcaaag caattaaaac
    58141 ataaacatag ttaatatgcg gatttttaag attaatagta ggtgtggttt cgaactgcaa
    58201 ataataagtc caaataataa ataataacaa agccatttcg gcgactataa agaatcccaa
    58261 gaatattgga tttaattgat tagtatagac aaaccacagc cttagtaata tcaaaatagc
    58321 aaatagccat aagcctagag atgatgattg caccggcgcg ccaggcgaat agccagctga
    58381 agaaaatata ttgataatgc ttaataatac aatgacaccc aactggacgc cggcaaccaa
    58441 tagttcacca gtgtattgtt gggcttctat ctctttcctg atgcgaagcg gtaacttaga
    58501 gtaactatcc actccttccc cccactatgc gttcgcataa ccctttaggt aacatgataa
    58561 atgcttcagg atcacgatcg gccgttgatt gcgcagcaca agaatgctgg gaagtagcgc
    58621 aatcattttt gcccgctctc gccacattat agcaacgctc tctttttact gtgatttcat
    58681 ctgatgaaag ccaatctgta taggtgtata tagaagcaag agcagctccc aaagcagctg
    58741 caatgacggc cgtgttttgt atcggacgtt tattcatatg attttcctgc catttttagg
    58801 agtcgctttt tattttaggc taaataatcg gcaacaacct ctccatacgg caaggtcaaa
    58861 tcagtaataa aatgtgaaac ggatacaatt tttttaatat aaagattaga gataggagcc
    58921 agtgcatgag gatgaagctc caagctacct ggtccggtcc atgctccttt cactgtaatg
    58981 tcagtcaaat aagttttggt caactgattc actaaaggag ttccatctac attaggaatg
    59041 tttttaagta aaaatacagg ctttttaacg gattccaata ccttttccgt atccagcgga
    59101 cgatgtttat atcccatagt tgcgatggca atatcaatac ttccatactt aagaatgcca
    59161 atgagtgtat cttcttcaac aaacaatttg ggtttggcca gtttctttgg aaacccccat
    59221 atttctcggc caccagcaat aggggcatgg caatcaagaa acattgaaat ggtaaatccg
    59281 ccctcttctc ctttatatct cacagggacc acttgccctg actcggtgta atcaccaaat
    59341 cctgttgaat caggcatacg tataaattca aatttgacta ccggctccaa taattccata
    59401 tcaggaggta aaatagcttg caaaagatcc ggatcagttt cataagcaat aataaaaaat
    59461 tcacgattaa taaatcgata tgggccagct gggtaagtat ttaaccaacg tggagctggc
    59521 atgctaaatt cattatcgat aaccccttct agagaatttg ctgaaagata cttgtccatt
    59581 aatcactccc tttgtatttc cttattatct tcaatgagcc ctaaacctcg cttgtccctg
    59641 atttattttt tttattcaat gaatcatcgg taatcagcaa attattggcg cgaaaagtgt
    59701 aataagacca aagccaattt aaaatgacac ttaaacgatt tcgagagcca accaggaaaa
    59761 agacatggac tcccccccaa aaccaccatg ccagttcccc actgattttg aaacgatcaa
    59821 attctgcaac agccgcttta cgacccactg tagccaagct accataatga atatatttaa
    59881 atggtttgta tcttgaattg ttattataaa ttcttttgga tatgactttt gctacataag
    59941 caccgccctg ttttgcagca ggagcaatac caggaaccgg cttaccattc caggcattcg
    60001 aagcagctgt atccccaata gcaaaaatat tagaatagcc ggctacggtg agatcatcat
    60061 tcactttcac gcgacccgct ggatcagctt cgagctgaag ccattttgat gcaggggagg
    60121 cagcaacacc tgctgcccaa aaaactgatt ttgaataaat tctttcctta ttaataatca
    60181 caccatcgct atcaatttgc tcaaccatac tattcactaa aactttaacc cccatgcttt
    60241 ccaaatagcg ttgcgctttt tgcgatattt gttctgaaaa ggtaggcaaa atccgcggtg
    60301 ctgcctgaac cagaataata tttgctgatg ccgggtcaaa atgacgaaac tcctttacta
    60361 tcccaaatcg agccagttct gctatggctc ctgcaagttc cactccagta ggcccggcac
    60421 ctacaatgac aaaatttaaa aattgcttgc gttcttcatc actttcagcg atctcggcca
    60481 gttcaaacga ctttattata cggctacgaa cagaagttcc atcgttaatt gtttttaacc
    60541 cgggagcata gggggcccaa ctgtcctttc caaaataact gtgcgttgct cctgtggcga
    60601 tgaccagata atcatatgga attgtgaaat tatcagctat gacaagacgt tcctctttat
    60661 ta
//
LOCUS       DASLOE010000004        55382 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.004, whole genome shotgun sequence.
ACCESSION   DASLOE010000004 DASLOE010000000
VERSION     DASLOE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 55382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55382
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.004"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            126..4616
                     /gene="sidE"
                     /locus_tag="U6309_000169"
     CDS             126..4616
                     /gene="sidE"
                     /locus_tag="U6309_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector NAD-dependent ubiquitin ligase
                     SidE"
                     /protein_id="HEN5503640.1"
                     /translation="MPKYVEGIELTQEGMHAIFERMGHPNITSGTIYNGEPTIDKGAL
                     DRQGFMPVLTGVSPRQDSGHWIMLIKGQGNQYFLFDPLGESSGKYYQNILAKKLPGAT
                     LSVIPNNAGLNMGLCGYWVASVGLRAYAALTQPIPPSLRNLGQTITQEMRDELTQDGS
                     EKITQWLRAVGNEFPEGDIQSDATALRRATEKNLRIDELQPVFAGTTPKEISINPTAP
                     QETSVPAWNGFSLYTDEIVRNAARYAYDNYLGKPYTGTVEATPVNFGGQMVYRQHHGL
                     AHTLRTMAYAEIIVEEARKAKLRGESLKTFADGRTLADVTPEELRKIMIAQAFFVTGR
                     DDEESSKNYEKYHEQSRDAFLKYVEDNKSALIPDVFKDEKDVKFYADVIEDKDHKWAD
                     SPAHVLVNQGHMVDLVRVKQPPESYLEYYFSQLQPWIGSKAAEAVFAAQRQFFHATYE
                     AVAGFNSENTEPHLVVDGLGRYVIGPDGNPIREELDDEDEEESGELKFFSQKKKLEEN
                     QRYMRVDEYLKLDEVQKRFPGAGKKLDGGLPGLSEYQYLQRLNSINRARCENDVDFCL
                     GQLQTAHHQTKITPIKRAFQSSSEKARRQPNMDEIAAARIVQQIMANPDCIHDDHVFL
                     NGQKLEEKFFRDLLAKCDMAIVGSLLNDTDIRNIDTLMQHERNTEFHSTDAKAKPVKL
                     GETWEKTIRSGGGVTQIKHDLIFLMQNDAWYHTRVNAIAQNRDKDSTFKEVLITALMT
                     PLTNKSLIDTSRSPAPKTLFRGLDLSEEFKNKLINQAETIIANTTEHLFTDLSTEAFK
                     QIKLNDFSHVSARTCASTSTNIEVPRTIFGSNTIFEILDPDGLLHPKQVGTHVSGSES
                     EYSIYLPEDVALVPIKVSFDGKTVKGKDRHIFTLVAVKSPDFTPKHEIGYAVGPLLRM
                     QTPKLEEIQRLVEQAREEPDLERVFNLQSRVARQAKLFSTESGYKTFLNEKVAPVLEQ
                     SLNGLLDNNVTILGKVLSAFPSDGQWSAFNSVEAMQMKIQMDAIKQMVEKKAVLEGRI
                     LPALAQCQDALEKQNIDGALQALKNIPSEKEMQTMLSISSGLRGQIQRAKQNLTENLE
                     PLQRAMTAKLVSDQEKVKVRYEKLIAGIPQQITALEKAALSDLAGVKKVVSHFNHLQE
                     ELKLLRNEKVRMHTGSEKVDFSDIAQLEAQLQKIHTKLYDAYLVELTKEISVLAKEKP
                     KNLADVKRMVSNFYAMSADMEQLRQEKIKKHGESKDPIDMSDIDKLKEELQKINQFLV
                     KAMGTNIRVSMNQMEVKTFDAQEKEAQQNLKQLDALINKLESSDAVQKQKEELEKLNQ
                     LLAEKRKAYPAMVQLQFRSEALIIHLRELCEAHQDHLAKTRKTRAQEITKGRWKVQWL
                     TDWVGLTSDERVTLANKEKELAKFKEDLNNDEYDLQELISNLAEKNPSELEEAIGISK
                     ESAQKLYKLLTHLNHSTTFMSKIEQRLQSIDELLNEFGKQSPRTEIIKTAEEKQGTLL
                     RL"
     gene            4915..6507
                     /locus_tag="U6309_000170"
     CDS             4915..6507
                     /locus_tag="U6309_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEN5503641.1"
                     /translation="MKKTGSDVLKEFITTFEINYIFGNPGTTELKFLEAIEQCDNATY
                     FLTLQESSTVGIAAGYAMITRKPALINLHNYVGLANAVCNMYNAFTSGIPMLIIAGQH
                     SRPYLIHSPVMSGDLTSLAHTAVKYAYEVNSASDLAVALQRGYAQAILPGPGPVFLSI
                     PMDIWQEETQETTIKKTRFLHACINAEIEQICEVLTNTPKGKLAFIADYEVGASNSIC
                     LLHTIADRLNADIYAAPYHVREVINPLSVNYKGRLSDKSGEVHNLLLQYETVVLLGEK
                     VKGYLYTGQSSIPQGIQFIQISAASEHLAFDYPCDLAILGNLEATLTAISKHLKINGV
                     NVPLKPATNIKNLEKKYKQHPLHSLIVTLLNQVNRSVHLITEGSSEDAVVQDIATTLG
                     FLNVNFSPRGGGLGWAMPLAIGISLATRHHSICFVGDGGSMYAIHSIWSAAKYKIPVI
                     FICFVNREYRVLKTDMSQNRSFTEEDFFRLDLNHPPIDVQQIALSFGAQVKTIKSKEE
                     VEAVLRSSFAFQGPTFITLYHQ"
     gene            complement(6625..7071)
                     /locus_tag="U6309_000171"
     CDS             complement(6625..7071)
                     /locus_tag="U6309_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN5503642.1"
                     /translation="MKHNLLDKAYKHCVNHGYRFTEPRERVLKILVDERKPLGAYDIL
                     QRLSMEVDNPKPPTVYRAIQFWHQEGFIHCIDSLKSYVACLHGHHVGQAQFLICNQCD
                     FVKELECVVDFTSVTEAANSIQFSIINCTVEIKGLCSDCNLTNLKN"
     gene            7403..9961
                     /locus_tag="U6309_000172"
     CDS             7403..9961
                     /locus_tag="U6309_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN5503643.1"
                     /translation="MLYSNYRFYLAKDAQLLNPKESREVIEQILTKTKELELIRVAVD
                     LTSSNKSISLLSASKENHVIKTKVTQILKEQGLSVIDMMEVEVLQDIEKIEHHHPTHK
                     SKNTHLHPIKKHQKAKHDHSHGHGHHHHGHHHENHWIKATLGLIWGIGLLVLSIGTFN
                     IPLVAYYVITGLTSLMTLYLGHTVYQSAWQALKEKKWDTTTLYSISTLTILAVSIASV
                     FVPGLPMMFEAAPLVLGFWHLGEGIEHTLVGKINKKLDVRDCVPPLVLLKGNPDKEIS
                     VKTLIPNDMIIVKKGQVIPVDGVLTQKALLYTTRIDGSPKLKEFHPGDVVKAGMQLAD
                     HIPSLEMQVTKTYQNSYLSLIAENINKANDEKAPIEMFADKVLKYFIPGLIAVAFLSG
                     IVIGSLFNPALAIQCVVSVLVSACPCALSLITPMAVKIGMKKASENGIHFNNGKALQA
                     AADIDTVVFDLNGTLTQGKMVVKSLHITDKKYLRPIALLESQSDHPVAKIIKSYIEQQ
                     DTASTEPLEITSIDKSHHSGMKGIINGEAFMIGNKDMLAANGITSIDKPYDNPEKGSV
                     YIVRGTTVIGQIALIDPLREDAIATVKQLKRLGKSVHICTGADQATAEKYAALLGIPK
                     DNICANTVGAVTKKGEVSKTSYIQQLKQKGFKVAMVGDAANDATAIAYSDIGIAVKSS
                     IGDSITEQHAGIVVQQGLLFPIATAFDVAQKTKQNIFQNLFVSLSYNSIITLVAAGLF
                     VALGFALNPVLGVALMVLESTIVLTNLYLFKQQDIVSSTSSNANNAVEVEPAANTTSK
                     VLHALGHRSQPQENLAILTESKPVNPKVINLFAPKEHSSYRSDVTQQKLGCGIC"
     gene            10108..12237
                     /locus_tag="U6309_000173"
     CDS             10108..12237
                     /locus_tag="U6309_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEN5503644.1"
                     /translation="MNNTWHQTATGNRSILNNLRLSRTLLNVIFIIALSLLLIINIIS
                     YNQVKNLIFASNWVSHTYEVIQTIDTCLYDVVEIESRQRFYLIRGSSPQLMTDIDDMK
                     STLQLNLYKLEQLTRDNPEQNKRIHRFIDLVKQRLNLLNQLVQLKGSGKLDTQEGYDL
                     LNQSQDMSHQVKSLGQEIKSVELVLLSERNTGAVRWADTSSFILISGGIASILFLTIA
                     FILANIELSTRRKTELQNQNTQIRLKKIIESASDMIAAFDKDQRFITFNEAYQREFKR
                     LFDKSISINMSLEEAFNDVPENKKKLMQTWKESLQRDEETQNIEVNTEQEKTIYEMTS
                     KLIQNGDNEIKGVVHSVRNITKRVQEHTELQESYEKLANGMKELQEKNEQITLLVEMS
                     DIMLACGSQEELSDVMAKYSQRLLQFSSGYLFIMHPSKNYLEKAASWGNPQPHDLTFT
                     PEQCWAIRLGRIHYAGSSRIELMCSHTMFAEQPELSLLCVPLMAQNDIYGLLYLEVGL
                     EFDENQQLLITAFAELTALALANVRLRENLRYQSIRDPLTGLYNRRYLEDFLFKQLHQ
                     AERTKASFAILMLDLDHFKKINDTFGHDAGDLVLKELGQILNSDIRLGDIAARYGGEE
                     FVLLLYDIDAQAAKMKAENLRSAISNLQVKYGAQPVGQITASIGISVYPDDAKSPAEV
                     IEAADKALYQAKNKGRNKVILFSEISS"
     gene            12447..13280
                     /locus_tag="U6309_000174"
     CDS             12447..13280
                     /locus_tag="U6309_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEN5503645.1"
                     /translation="MFSKALISATYKNGKPGQLTEEHEKAEHHRFKTEYLFKNAEIPK
                     DLYASRLIQHFLIIKAIETKLQNLPKTEQSEISAFFALSYLEHLWRTPAIENDLRQLN
                     INPDEIHKDEITKTTENYLENIEKFTPKILLAHFLLHIAGFMHGGNIIRSKYIEPSNR
                     LTTYQITADQYDFSAAISCLSSGKHSPLALYQDMMKQIDNIVLTGDEYQELLEQCKGI
                     YETMSNIYDDLCDMHTHHLKLPTYSLAAISVSLVALGLILKLLMDFLNPMTNPITSAP
                     R"
     gene            13428..14993
                     /locus_tag="U6309_000175"
     CDS             13428..14993
                     /locus_tag="U6309_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN5503646.1"
                     /translation="MLKRDISTLNILVASAGGMIGSGWLFSPFISAQMAGSNSLISWV
                     IAAIFMLFIALPLCELGTMFPVSGGMSNYPTYTHGREVGFLFAWTSWLSYVVMTPIEI
                     QAILQYSSHFFPILIDNHSESLTLSNYGYLAAIGIMFFVVILNSYGIKFLAECNKYAS
                     IIKFVLPSIAIISLLKQSPSLANVNINLSSKEHWVEIFTALSAGGVAFAFTGFQNGLI
                     LAGEVKNPQRNIPIAILGAVLVGFILYFMLQFSFIAAVPQKYLAQGWHALSYPGDEGP
                     LVGLTLLLGLGIVATLLLFDAAFSPFGTTLVYTAATSRILYGMALNQHLPKFFLKVNR
                     HNIPYVTLYANLLVGSFAFLPFPGWQKLVAFLSSASILSYGIGPICLLAMRKMHPHMD
                     RPFKLANSIFLSHIAFYICNLMLYWCGFSIIWKLNLALLMGLGIYFFYHRHISLRNGS
                     PLVWFIMYMGSFSIISYLGSFGGIGLLKFPFDLICFLPYSILILYMSQVLVTPRHDND
                     MVSNVTELPVYNS"
     gene            complement(15164..16267)
                     /locus_tag="U6309_000176"
     CDS             complement(15164..16267)
                     /locus_tag="U6309_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5503647.1"
                     /translation="MSYSIDNPGGGDCGFYALAIGLISIIQNEYKLYGKSKTYDRWKQ
                     VEGIYGVRLQDILNVDLTKLHDSPYTYKSELLFLLQMSLRNIAVIVNKEDLVKRIIEE
                     GRSKDQTKIEGSNAYCKFMELVQLYLYRRDSLERISQFNELALSPEIVQTARDIAKIL
                     RPILRNQPFHQVQKIENAFVKEVLLNDVLSGNTINSNSIILKNLEKIKEQGRWATHDD
                     LKEIADLLKINLNVVGQPNGKPIPEYPTVTLKNLSNTHWITQVESLQGPRQYTPRKRA
                     FGYEALSDRAVSLKEEQVLERTFKTQTQSERTKRYHQHFETLFDTLSPYGFFPHGQNK
                     RNTHVSGRVQTGLESDESLATRLQEAELRRILY"
     gene            complement(16450..17847)
                     /locus_tag="U6309_000177"
     CDS             complement(16450..17847)
                     /locus_tag="U6309_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEN5503648.1"
                     /translation="MICFPSGKNRLIVLCLFLMMSFKCFPIPGKPLLLDAVLKSVDRS
                     YPQIVIARLEIRKAEGDYVNALGKFDPSFDVNPRFQPVGGYINNYADNLFNVPTLYNG
                     LKLFGGYRIGRGDWPIYYQNYLTNSGGEYRAGLSLPLLRDRRIDKERTELFTREETIR
                     MKQQDAAATKIKIYQETIKAYWQWVEAGFQLRTFRHLLNLAKERQNAIIQQAQQGDLP
                     RLAIAENLQLIVQREQLVNQGEMLLAQAGINLSLYYRDANGKPQKPDESQLPATFPAQ
                     PIRVSNSISQLQRHPVLRKLENYSKIVKLKQNLARNELLPNLDATAYTFKQYGTGGYP
                     LLIPQAAMIGVNFKFPIFQREARGKLISTTSELQQVKTEMKFTYEQLNNELSNLLVAI
                     RIYQQQVVLLNKELNLARQVEKGETKRFYEGDSTLFLVNQREQTTAQVKLNWINAEVN
                     LQEATALARFFSSTQ"
     gene            complement(17844..18830)
                     /locus_tag="U6309_000178"
     CDS             complement(17844..18830)
                     /locus_tag="U6309_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEN5503649.1"
                     /translation="MKLHAYSMISKLPKPQKIGKIIFIVFVAIILFLSFTPWQQFALG
                     NGKVIAFSPTERQHTVNSPISGRIKKWYVDEGMRVKPGDPIVDITDNDPELLSRLEVE
                     KKAILLRIEAAEQAIAAGKANVDRQKKLYEQGINSRRQYELAQIEYAKYQNELAQANI
                     DKVNVDVRIARQKTQIIKAHVAGIIFRRLTGQESVVVNAGDVLAQILPETESRAVELW
                     VDGNDIPFVRLNQKARLQFEGWPAIQFRGWPEIAVGTFGGTVSFIDPTDNGFGLFRVV
                     IVPNEPWPSVRFLRQGVRVHGWIQLGQVPLWYELWRQYNGFPPESDRENKAS"
     gene            complement(18827..20416)
                     /locus_tag="U6309_000179"
     CDS             complement(18827..20416)
                     /locus_tag="U6309_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN5503650.1"
                     /translation="MNAKPINSIIDVLDRQTISSIAMISVIISLLSLSIPIAAQTLVN
                     LIAFGKLLQPVITLSLMVLILMMALGALHIWQIVIIEVIQQKLMVKVSLSLTRHFTHM
                     SLDNFSTHHGPELVNRFFEIVTIKKSLASLLLYGINLGLQLFFGLLLILIYHPLFLLF
                     DIFIILGILLIIFIPYKKALDSAKKECSEKHVVGAWLEEILINRYLFRFSLYHRYVTQ
                     QTDKKLVAFLKARNTHFKQLIKHQIGFYTLSALASSLLLGLGGYLVINNQLSLGQLVA
                     AEIVLGALIYSFRRFGALLENYYDLVASENKIDAVLNLSLEQVKEDEHGELLTPINSI
                     QLEIDSKNKALITVDKPLLVFSEKSDLCQLFVEQLFGFQDPASLHVLINEIPCARKNL
                     ISLRRFSLLIAEPQWFTGSIYDNLVLNHRNVPNKIIIEQLKNVGLADKIIQLPEGLRT
                     ILYEWQDVFTSVELTKLMLVRAILLQPELIVIDCAFDILNHQEVNEMMELVLSLKNTL
                     LVIVSQRSDFNYLNNRLVISS"
     gene            complement(20458..21309)
                     /locus_tag="U6309_000180"
     CDS             complement(20458..21309)
                     /locus_tag="U6309_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN5503651.1"
                     /translation="MSIDTITLQCFLAVAETQSFTKAAQRVGRTQSAISQQIAKLENL
                     IEKPLINRGRELSLTTDGEIFLGYAKRIYELHRESLDRFKAPELQGEIRFGLPEDFAS
                     IILSDVLVEFSRLHPRVLLNVECDLTLNLIERFNEGKFDLILIKTTQQQIGTECIEVC
                     IEPVEWIGKKELLPLLDKPGPIPLVLSPTPCVYRGNVIDALDKAHINWRLVYSSASYA
                     GKMAAVRAGLGITAIQRSMIPNYLERLDYDFLPILNDVHVSLLKNSESNKAIESLEFF
                     ILQKLKY"
     gene            21411..22931
                     /locus_tag="U6309_000181"
     CDS             21411..22931
                     /locus_tag="U6309_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN5503652.1"
                     /translation="MDKLLNGLSISLMLSPLIAMLFYTLLGKTRNIQATYYGTTVIGF
                     GFGLSLLALMISANTVNQNSYFLVHASTLSLLLTTLILFISFIIHRFSIRYMHGDKCY
                     RRYFFKLSAITLTTVVMALADNLLLFWLAWSVSNLLLINLMIHKSQWNAAYNSGILTL
                     KTLLPGSLSLITAFCIFFALNNTLSIEIITSEVSNKPSPAIAVALGFIILTAMTQSAI
                     WPFHRWLMSSLNSPTPVSALMHAGLVNGGGFLIVKFAPLFATQANLLTILFVFGALTA
                     LLGTLWKLLQWNIKSMLASSTMAQMGFMMMQCGLGLFPAAIAHLCWHGLFKSYLFLNS
                     GSAIQQKKYQNKSGPEKLTTLLIAFFGGLLGMYGFALITGKPILSLQTSTFLLGFAFI
                     TGTQVTMSLIANEKHFKKGILALALVFSIGVFYGGSIHIIESLLPNPTGFPQHQLSSL
                     QGLTLIIFFSLWIVFSMGLFKKIRETRLWDWFYMYMFNASTPHPKTITANRHTYYY"
     gene            22946..25234
                     /locus_tag="U6309_000182"
     CDS             22946..25234
                     /locus_tag="U6309_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HEN5503653.1"
                     /translation="MSSHITLIKTNKDNQNQMTQCNYPIDNDVMIIQAMVNNVAKQIT
                     PVWPLEKFIACNSLHGFESMSFEEAILINQTAKKGTPFNEKLERVNWHMIKWCGSFLD
                     IGQGTIEMPHRDKGLYFGFLKLAPFDSALHQNNKSTKSWLSNLPEMPEQAIRLCLDKL
                     GVLKQRQEEFLQSTLSHLPGWAGYIKWISEWKNRNGKEENPVSLVDFLAVRLIITCVL
                     WPEANLEEKKKEKDSTHTKQLIQNIKHKEDDYRQLLLKKLLPELNKVQIKENRANAQM
                     VFCIDVRSEPFRRCIEKLGNYETLGFAGFFGLPVSIKDYDGETIKDSCPVLLKPRFNI
                     HEKAIAANEHCIEHHEKGKEIKKILNRVYQQLKYNFSTPFALVESLGIWCGITMFLKS
                     CSPIFARRITKNLNEMICPSIQTQPVFELDLLEKEVGISLQEQIAYAEMALRLMGLTD
                     NFAKLVIFCGHGSSTQNNPYASALDCGACGGNQGGKNAQLLASILNKITVRRALAENG
                     INIPQDTVFCGAQHDTTTDEVEIYHSNVSQFIDQDILDQLRTDLKMAKHNNNLERINY
                     LNSIDCPEKDIVRRSTDWSETRPEWGLARNAAFIVAPRQLTKNIDLEGRCFLHSYDWT
                     KDEDGTLLETILTAPMVVAQWINTQYLFSTIDNVAYGSGSKITHNVAGKIGVMQGNAS
                     DLMHGLPLQSVMSYDDKSFHEPQRLLTIVYAPREIISELVEKHDVLKTLFFNEWVHLV
                     AIDPRSHLFYKLEKTNTWSVIQ"
     gene            25248..25601
                     /locus_tag="U6309_000183"
     CDS             25248..25601
                     /locus_tag="U6309_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEN5503654.1"
                     /translation="MNIIKVSEKKEMNISLHPMKKIEVIVSGENENMVANMMEKANIS
                     GFTLLRNVSGKGHHGFHEGKILFNDKASFVMFIAVAPEEAIAKIALGMKTLFQKNSGV
                     LFVSDVSVARVDYFS"
     gene            25626..26465
                     /locus_tag="U6309_000184"
     CDS             25626..26465
                     /locus_tag="U6309_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503655.1"
                     /translation="MHYQNQRVLLASKHQKEKAIQPPFEEKLGCFIHVPADYDTDQFG
                     TFTGEIPRKASAYETLILKAKAAAQQFGYRYVISSEGSFGPHPQLYFCPADTELMAFV
                     DLDNDLIIAEHELSTETNYSHIDISATDDYEAFLSKAKFPSHGIIVRALNSEENYIQK
                     GICELEQLKTAIQKAFTYSTIIRLETDMRAMMNPLRMNIINKLAVKLVQRIQQHCPQC
                     HTPGFGKISAEGHLACISCGTPTELYLRKVLNCLKCEFKTYQARDDGLEFADQRYCPY
                     CNP"
     gene            26525..27025
                     /gene="purE"
                     /locus_tag="U6309_000185"
     CDS             26525..27025
                     /gene="purE"
                     /locus_tag="U6309_000185"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEN5503656.1"
                     /translation="MKPILIGLIMGSQSDWQTLIHAAQTLDALNIGYEAEIVSAHRTP
                     DKLFQYAEQAEERGLEVIIAGAGGAAHLPGMVAAKTSLPVLGVPVMSQTLNGVDSLLS
                     IVQMPAGIPVGTLSIGKAGAINSALFAAAILANKYPDIRAALKRYREQQTQKVLDNPN
                     PKEEKS"
     gene            27022..28101
                     /locus_tag="U6309_000186"
     CDS             27022..28101
                     /locus_tag="U6309_000186"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEN5503657.1"
                     /translation="MKLGILGGGQLARMLTLAAYPLGINTVCLDPSPDACAGQLTKLI
                     VSDFNDELALKQFLSEVDCVTIETENIPFSCAELAAQTKPFYPSTQALKITQDRLYEK
                     NFFRTLNTPVPEFLPVESEDELMQAISVIGMPAVLKTRRFGYDGKGQYVLRTQADISK
                     SWSLLGSQPLILEKLISFESEFSLIAVRNKSGETSFYPLIKNHHRKGILHFSEAPVHH
                     DTLQQEAQIHAFKILEALNYVGVLTIEFFYQGNQLIANEIAPRVHNSGHWTIEGAHTS
                     QFENHLRALFDLPLGSTEVSGSCFLLNCIGNLLPLEPCLSIPGVHYHAYGKAPRPDRK
                     VGHVTLVDTHLERYTKSKDLLLDIS"
     gene            complement(28187..28399)
                     /gene="yacG"
                     /locus_tag="U6309_000187"
     CDS             complement(28187..28399)
                     /gene="yacG"
                     /locus_tag="U6309_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEN5503658.1"
                     /translation="MNNQQKIKCPICGKQNTWSPDNQFRPFCSERCKLIDLGEWASES
                     RKIPGSSIDPESIVTTNNKQDNVDEQ"
     gene            complement(28522..29406)
                     /locus_tag="U6309_000188"
     CDS             complement(28522..29406)
                     /locus_tag="U6309_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN5503659.1"
                     /translation="MEFKLLQTFIEVVRQGSFSKAAENLFATQSTVSKAIKQLEEELG
                     ITLIERFKKRNVPTTAGEIVYQRGTKLLADRDDLLKELDEIRGLKQGRLRLGISPVGS
                     STLFAPLFVLYRQRYPGIEVELVEHGSDKLAECLRAGEIDFAGTLLPISEEFDCQLVR
                     SEPLVALLASGHPLATKHCISLPELKEIPFILFGSGFALHRIILDACRHAGFEPKVVA
                     QSSQIDFMLELVAAGLGVTFLPRMIATQRHTTQIRSLLLNGSEFQWNMAMTWRRNSYL
                     SEAAKAWLALVREVHVGN"
     gene            29512..29901
                     /locus_tag="U6309_000189"
     CDS             29512..29901
                     /locus_tag="U6309_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEN5503660.1"
                     /translation="MANKIQQNSFFQIVLVGLFWLASELLLKLVKLPLSGGILGLGIV
                     LLLLATNCLKLNRIRRGAELLLANMLLFFIPGVLAILEHREFIGLLGLKILFIILLST
                     ITVMLVTALVVDYCYRWRTHHAKQHSL"
     gene            29879..30586
                     /locus_tag="U6309_000190"
     CDS             29879..30586
                     /locus_tag="U6309_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEN5503661.1"
                     /translation="MPSSILSESMIQAFFWSLITIGTYFITQKLYRRWPFWWLMPLAL
                     TPILVASVVLLLHVNYQDYFRGTKWLVLLIGPATVAFAIPIYEKRALIRQHWPVLLIG
                     VIVGSFTAIASSWGLAILLGLDDNLRLSLLPRSISTPFAIEISRGIGAAPDLTAIFVI
                     VTGILGAILGEFILSRLPFNSALARGALLGVGAHAAGTAQAHKIGNTEGAIASLAMIL
                     VGLFNILLLPLISHLLK"
     gene            30681..32096
                     /locus_tag="U6309_000191"
     CDS             30681..32096
                     /locus_tag="U6309_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEN5503662.1"
                     /translation="MTIALVWFRQDLRLTDNPAFIEACFHHEIVIPLYIYDDKCSVLG
                     QAQAWWLHHSLIALGKSLNQQGLSLVLRKGSPQEIILELVAQYGVESVYWNRCYEPQV
                     IARDKKIKLALGEREIKVQSFNGNLLHEPWTIKNKNGDFFKVFTPYWKQCRQILNIPS
                     PFMLTKRPRGIEITSDDLGKWKLLPAINWAARFSEYWTPGEEGAQKKLDEFIVHNLSG
                     YKRERDFPAKNATSRLSPHLHFGEISPWVILRALELAKLEQTCDLASVEHFLSELGWR
                     EFSVYLLYHFPKLDCENFRKEFDAFPWQNDEQLLTCWQKGMTGYPIIDAGMRELWATG
                     YMHNRVRMIVASFLTKDLLIDWRSGAQWFLDTLVDADLANNSASWQWVAGCGADAAPY
                     FRIFNPVLQSQKFDPDGIYIRQWIPELAQLNSQSIHQPWTDADANSIFLGKKYPKPII
                     DHQEARTKALSYYQQIKKGMD"
     gene            32129..32602
                     /locus_tag="U6309_000192"
     CDS             32129..32602
                     /locus_tag="U6309_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEN5503663.1"
                     /translation="MNKKSWTELIAWIITFEAIGFFLGMLTQANIPSWYEELNKSGLT
                     PPGPVFSIVWSILYALLAIVGWVLWCSRNESEMRSVFYLYSVQMLMNWAWTPLFFVLH
                     WTGLGFLWILVMAGLTGLLILSLKNKKESIAILLLPYLVWLLFAAYLNGMIWLLN"
     gene            complement(32850..33473)
                     /gene="ravG"
                     /locus_tag="U6309_000193"
     CDS             complement(32850..33473)
                     /gene="ravG"
                     /locus_tag="U6309_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavG"
                     /protein_id="HEN5503664.1"
                     /translation="MKIIYPGRVICDMKNAAIIKNRIFLNLDKPVKRFLASDKADTPM
                     TAEFYAEKDYEQLFLDFLSQATGSYDEQISMLIAELDSGADRVAQKLMSALYSPWQKN
                     LFPKAIKTIANKSEEYPLMSDLLIKFCQQHVGSVDAVDDFGETALAKILKKDQQRKSP
                     LLFLVKHGAKHCQLTSALQDSLIVNNSDIYNVAEDNTMDWISNCPQP"
     gene            complement(33518..35488)
                     /locus_tag="U6309_000194"
     CDS             complement(33518..35488)
                     /locus_tag="U6309_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503665.1"
                     /translation="MLSKEKINLSLKNTKRQGSDNRAALADPTSLKNTGDYRCFNSKP
                     EAYTYFIFLLNQWQTNYIGDKREAIAKAIDSIKMAYENHEQDTLVVHKTLHGLSHLLE
                     QEHADELKALCLVPIKDSQSNANNILPRRFESELACRLIESPTKSMMQAIDLVSQAII
                     NLIEEYSKLDSKSSERAALNFFLQELEGSDIEFGAFKKRPSLEEVIAILKENNPKNFI
                     QIQFISFMFARSVIGPFPLRTLPPFKPTGLFAQVLDKYLQDHPDLKEEEEGFSYQLIA
                     AKNEAEEFRTFITKKLFYQDELYTQGENRGRQGPRKTYLKTNQLGLMKLDHSAHSRGL
                     TVIPEQTWCADAQAQEANQNSIYYKTALTNDCPYVTGPSGMTSIYMNMMLLLLNPQNI
                     ELIQAYSLGIIAYIVGAGFHSVNEILIPLVKCINLLPNYPSYEGLEYLTHPPLYHEYF
                     KQIAQFDSDFIQLRDQVWAYYLNYFKSCYMPLCMRDFCEAEQLANCPAPAPEVKILCD
                     IVLKSIEDCINFHNLNRHLDSRFLGTTTADIDALNQLKEACASQSSISGIMRLLQKYF
                     SGENIARPTAMHKFETRSYIRFFLDALKDNPQIISHINEKMKLDPPLVISTKAEIFSD
                     DFRIECFNMHRILKREHQVSENCNNTSQFLKL"
     gene            complement(35507..38392)
                     /locus_tag="U6309_000195"
     CDS             complement(35507..38392)
                     /locus_tag="U6309_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEN5503666.1"
                     /translation="MKLLRFHELKSLPGMDEKALELLIKVLGNKGIRKLIKSADGKPI
                     SREIMIHEFGIDCQILFITTEASLKPIIVPTENKISGGGKSYCEQFKVYALDDGKTYF
                     LKSVKINAESLTEFTNETDTLSKLGRLVGTFFNEQTQVHYILTTFIKGIDLSRYKNAL
                     PLNINLKHFWEVLGIMISVCHQVKQFHELGLIHRDLKPGNIMLDADMQCHLVDFGSSS
                     SDKEPKPVSWGTASYLAPELNAQEDFIAFSQASDLFALAYSLDELFNPFRQVKFAKVD
                     IGIKNKHLVLLHAEIEACITGLMSNETSVRTLYFSRILQLQRVPESFKSRPEAFTYMI
                     MLLTQWKSCYETPEMNKELEEIIAQIKVAYENHEQDVVKIITLLEQLSKAGGLLNSHK
                     ALLSVLIKSLANVQQQELGHDDILPRRFESDVVSRLIKTPTAKMMAAIKQVSDAIVKI
                     LEQYEHSPTESFEHEVLQDFLEQMVQSDILFGAPKEKIEISDIIKILKANRPEDFVQI
                     QHISFKFAQVALRDLALHDLPKHEPKGAFLEILQKYQAKYAPEETSLFELYHHMAGVR
                     DHNPSKVKKGEGYRFRAFICDELFYGDEIFTTGDNRGREGKPNQRLQTNQVGLMKFEH
                     SAHTKGLLTLNGQSWYADCKTQLPNYDSIHYLSALKTDCPYITGPSGMTSLFMNMMFL
                     LLNPKDQDVILSYSLGVMTYVVGAGYHSIKEILVPMVKCVGIVPNYPQHKGIEYLTAS
                     PLYNHYFKAIEEFDKEFAEVHEKIWQEYLAYFRMTYMPVCMRSHCLPHQVPSMEDISS
                     EVKEMLEIVSKSVKNCLEEHKVKRTGDMGLFLSTPCGQTKALEVLSHVCQQQVGLTAI
                     FRQIQAYFKGTFETEEGIIINREMQLKFIAHFFDVLKESPTLLSQFNLTLRIENTIGV
                     HQCELGSSKQKELFLEIKKTSIAANEEQMVANHEPRKENRNVIILPY"
     gene            38756..39169
                     /locus_tag="U6309_000196"
     CDS             38756..39169
                     /locus_tag="U6309_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HEN5503667.1"
                     /translation="MNGWTLKVFAIAFFIFIVTDMIWLGFIARNLYFDQYQQWLRLSE
                     GQLKPIWWSALLVYLLFALSIVVFVIPLAKTSLPYAALYGALLGAIIYGIYDFTCLAI
                     FKDFPVGMAFLDWLWGIVLCSWSSIATIYVAGYIK"
     gene            complement(39174..40712)
                     /locus_tag="U6309_000197"
     CDS             complement(39174..40712)
                     /locus_tag="U6309_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HEN5503668.1"
                     /translation="MTTLCFILGDQLNETIASLAAINKQKDIVFLCEVAEETSYVPHH
                     PKKIAFLFSAMRHFAQQLKKKGYRVRYTAFDDPRNQGSFAKELLRAIVEEQVSAISLT
                     EPGEWRVLKIFHDLKSQLTIPVTLHEDNRFLCKINEFRNWAKGKKQLRMEFFYRMMRQ
                     KYHLLVDNDGKPVGGSWNYDAQNRKNANHISSFPKRLEHARDVITDEVLGLVSAHFAK
                     HFGQCYPFNLAVTREQALEEADYFMEHFLCNFGAYQDAMLKDEISLYHSKLSSYLNAG
                     LLLPLELCQMAEQAYFSKQMPLNSVEGFIRQILGWREYVRGIYWLFMPDYGEMNYFNA
                     CRPLPSFFWGEETGMFCIKEVVRQTSTEAYSHHIQRLMITGNFALLAGLDPKQVCEWY
                     LAVYADAYEWVELPNTLGMALYADGGLMASKPYAASGKYIHRMSNFCQSCYYNPNDLL
                     GEKACPFNALYWNFLHQNQEKLKDNPRLHYAYMNWQKMDFDKQKKTIHKAQRILADLD
                     SANL"
     gene            complement(40729..41427)
                     /locus_tag="U6309_000198"
     CDS             complement(40729..41427)
                     /locus_tag="U6309_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN5503669.1"
                     /translation="MARYLVIAASSAIGQSVTTLLKNRGDTVFTTARDNHKINPDFQL
                     EATHFDAVLDVFHQTGPLDGVVNCAGSLLLKNAHNTSFDEFQTAINASLTTSFATIRA
                     AGLTMNQGGSVVLISSAAALVGLANHEAIAAAKAGIIGLAQSAAATYASNNLRVNVVA
                     PGMINSPLTSSLLSNQLVLNASKAMHALGRIGLPEDVAHAIVFLLNPDNSWITGQVIA
                     VDGGLSRVRPKMKI"
     gene            41510..43297
                     /locus_tag="U6309_000199"
     CDS             41510..43297
                     /locus_tag="U6309_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family EDD domain-containing protein"
                     /protein_id="HEN5503670.1"
                     /translation="MPLDKLTGITFHAAIIAGCHRLIRHKQILNDINVFPVADGDTGD
                     NLSSTGMAIIEFSSAKTKLRGTLESIADASIIGARGNSGIIFSQFFNLLAKHLPLKEE
                     LYFNDFSKIMVQVGKEIVSVVTNPVQGTMITLIQKWATVCEIKQPDSLLAQAMLELLP
                     LLSVEVEQTKNSLNILQKANVVDAGALGFYYFVEGFTQFLQQSHTTDTANTHSPLSIT
                     EYPQLTCTTTEYRYCTEAVLKSASIDKNKLLHFLKAQGECASVTGNDRITRFHVHTNN
                     PREIFTSLYKDYTIQYPKVDDMQRQHELQFGKKYKIGLVTDSSADLPQDLIDEYQIHQ
                     IPLNIHVDEHHLLDKHCFEANYFYTQLNSFRTYPKTSCMNPNLIEEKLAKIASHYDHV
                     LVLSISSKMSGTYDAFLTATKKYTNITVLDTKMNSGAHGLLLHYAGQLIASGNDIDTI
                     AKLLGKAIKNTYIFVMVNQFESMIRSGRVNKISGRIAQWVNIKPIVSIDSAGQGIMIG
                     KCFSTSRARNKLINFLQEKKDEAQLILEDYCIIHAGDKNQALHLAEMTTQVFNKPPTY
                     IEPVSLAIGLHAGQGCIAIAGRLRERNDS"
     gene            43287..43823
                     /locus_tag="U6309_000200"
     CDS             43287..43823
                     /locus_tag="U6309_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HEN5503671.1"
                     /translation="MILNPLHIVHYLSYYLCWIFCIYLASYGYPLLGLIITVLIMGLQ
                     IIVQILNQLPWREALYFSIGLALLGAITDTIWLYSGMIYFKANFFGSHFPPLWMVALW
                     LSFGFNLVVLYGSWLTYYFLWGLVMFPSIIFAYWLGVQFGAATLIGSEIVFYLTLGLT
                     WSLFLPLSLYLFNYQHGK"
     gene            43820..44605
                     /locus_tag="U6309_000201"
     CDS             43820..44605
                     /locus_tag="U6309_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEN5503672.1"
                     /translation="MNKMIILLVIAIIFLHMCLIWLWYRYTNNPSVVDVGWASGLTLA
                     GLIYLNNQEFSLRSLLLSLALLTWGIRLGGYLWWTRIRLKKIDKRYLALSNDWKLAKP
                     LGFFLNFQLQGVLICLVSIPWYFSSSETQTTLNLLDGLALIIFLTALILETVADSQLQ
                     DFKENYPGKVCNQKLWRYCRHPNYFYEWLVWCSFTLFALSAPYGWIAIISPLTLYLIM
                     TKITAPMTEQGSIESRGNLYIEYQKVTPMFFPKWLKNNSTPRK"
     gene            complement(44606..45595)
                     /gene="cydB"
                     /locus_tag="U6309_000202"
     CDS             complement(44606..45595)
                     /gene="cydB"
                     /locus_tag="U6309_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEN5503673.1"
                     /translation="MLPFIFATLLGFIVLMYVILDGFDLGIGILFPFTSSESERDKMM
                     NSIAPVWDGNETWLVFGGAMLYGAFPQVYGLLLPILYMPIMLMLIALIFRGVSFEFRF
                     KAKRSKTLWNWSFSISSVAATFLQGVILGAFVQGFSINESSMTINDPDWLTPFSLCTG
                     IALVCGYALLGSTWMIIKSTGRLQRKMVHYARGLLVLVSFFLLFVSIWTPLHSEEVFH
                     RWYSFPNMLLLSPLPLIALMAIYLVWQNLNSGSEYKPFIYSIIIFLCSYIGIAISVYP
                     YLIPHHVTLWEAAAPDSTLVFILIGVAIMLPILLGYTLYAYYLFRGKSSENYH"
     gene            complement(45595..46965)
                     /locus_tag="U6309_000203"
     CDS             complement(45595..46965)
                     /locus_tag="U6309_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEN5503674.1"
                     /translation="MLVELLSRIQFGFSIGFHILFPTLNLGLAVFLVIMEALWLKTKN
                     PVYLEICKFWTKIFALTFGMGVVSGIVLAYQIGTNFGPFITQFGNVLGALFAYETLTA
                     FFLEAGFLGVMLFGWKRVPPALHFIATLLVTIGTTVSAFWIMSANSWMQAPSGYQLIE
                     GKYVVDSWWAVVFNPTFIPRFIHMLLASYVTTCFVIMAVSGFYFLRKQSTAIAKKSLS
                     FSLWASLILVPLQIGVGDVVGINVHHYQPLKTAAMEGIWETQKGAPLLLFAIPSQTQQ
                     KNFYEVGIPKLASLINTHEWNGEMIGLSSVGNEEQPRMAPVFFSFRIMVGIGLIMLFT
                     AIAGFILRLRGTLFENSWFHRWCIVMAPLGFIASIAGWLTAELGRQPWVVHGLLKTRD
                     SVSAIGVEEVIISFVLLIVAYGVVFGFYLYYLLRLISHGPGILEEDEAEHHAFQYMTD
                     FPKEKK"
     gene            complement(47078..48112)
                     /locus_tag="U6309_000204"
     CDS             complement(47078..48112)
                     /locus_tag="U6309_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503675.1"
                     /translation="MTIMRVFLSFLLLFFNSLGFAQSEFPQLFLFLGGDRASTFQETL
                     QNPCIRGVQIIYSWRQLEPQKGVYDFSKIEEDLNDLNRIDKKLYIQIQDRSFSPDVFP
                     VPDYIRLEKAYHGGVVQQYDFPGEGKPVTAGWVARTWDPAVRKRFQLLLKKLASKFDG
                     KIYGINLPETSIDLDPYNLPEDFSSDEYFYSVLDNMSYLKKVFQKTRVTQYVNFFPDE
                     WNNDHHYISRLFAFARKHHIGLGGPDVVPYKESQMKNSYPFFHKFKGKIEIAMAIQEP
                     DYTYTNPKSGNFYRFDDFYSFSKEYLGAFILFWNTEEPFFSEQLLPKLNGSYFKCYKA
                     NNEANAADTN"
     gene            complement(48491..49627)
                     /locus_tag="U6309_000205"
     CDS             complement(48491..49627)
                     /locus_tag="U6309_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEN5503676.1"
                     /translation="MKRILGSLGLAVVSISLFAHGSMEVPISRTYQCYKEGPESPKSA
                     ACKAAVQVGGTQPLYNWHEVNQAAANDRHRELIADGQLCAGGRDFFKGFNLARTDWTA
                     TVVHPDSNGHFQFVYVATAPHKTKYFKFYVTKDGYDFNTPLKWSDLETSPFCTITSVT
                     LANGRYQMDCPLPANRTGKRIFYVIWQREDSPEAFYSCSDVFIDGTAQAPTWFELQNF
                     YGSGDIAANTKITLRVFKNQVEVESTSITANESNRTAEQWPLALGTAVNSGSSLVRIG
                     QLDPQSGQVVLAANQANKIYLSDNDISSYRVLVDFVEPSGNSDVIYPDGISTYKAGTI
                     VLGRLDRKYYQCKPWPYSGWCSQAPAYYEPGVGLAWREAWILLG"
     gene            complement(50185..50502)
                     /locus_tag="U6309_000206"
     CDS             complement(50185..50502)
                     /locus_tag="U6309_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEN5503677.1"
                     /translation="MTMMITTGNSFEGKVIKQYLGIVRGIVVRSPTISQGLMGGLKSI
                     VGGKIGAYSQMCEHAREEAFQLMIEHAQALNANGIIAMRYDTGEIGQAGTEVLCYGTA
                     VII"
     gene            50971..51975
                     /gene="ravE"
                     /locus_tag="U6309_000207"
     CDS             50971..51975
                     /gene="ravE"
                     /locus_tag="U6309_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavE"
                     /protein_id="HEN5503678.1"
                     /translation="MEHCMPKTKYALPPVVLYESHADRATSDFLIKQLPDLKKAGYTT
                     ICVDGMEPGASLEENISMMKILIKIQIKKLSELPLEHPEYEQGIEKLRSVVAKLELFE
                     AMKEQGFKLGGIDLPVSEQLKEKSLNSIRREQTITDNTLRHVKENDGGVVVVLGFGHC
                     IFQQMIKEQDENANQYLWYHVHNPDNETQAYKELIKSYTKKGLSTYFPLGVNIFKSSD
                     KELDTDFWNKVSANCYNYDPKALETSTASILKSLLGPEVSAHLRTDGQHHVDALISLE
                     TVEKKHQVKSSDFLRSLSKTLGDIHFEVAKIKTKDQVIIRGINEPEVAEQISKLSKKM
                     "
     gene            52106..54355
                     /gene="katG"
                     /locus_tag="U6309_000208"
     CDS             52106..54355
                     /gene="katG"
                     /locus_tag="U6309_000208"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEN5503679.1"
                     /translation="MFKRTIPLFAAFTLAISPSVFPNDAHAQEDKPKTNQYWWPKMLD
                     LSPLRQPNATSNPMGEKFNYAEEFNSLDLNAVIEDLKKLMTTSQDWWPADYGNYGPLF
                     IRMSWHAAGTYRIYDGRGGANGGFQRFAPQNSWPDNANLDKARRLLWPIKQKYGRKIS
                     WADLLVLAGNVAMESMGFKTIGFAGGREDAWEAININWGPEGKWLESKRQDKDGKLEK
                     PLAATVMGLIYVNPEGPNGVPDPLAAAEKIRETFGRMAMNDEETVALIAGGHAFGKTH
                     GAASGKYLGPAPEAAGIEEQGFGWKNSYGSGKGKDTITSGLEGAWTVTPTHWSHNYLQ
                     NLFNFNWVKTKSPGGAIQWVPENSNASSMVPDAFDPSKRHAPVMLTTDLALKFDPVYS
                     KIAKRFLDNPKEFDDAFARAWFKLIHRDMGPRSRYLGSLVPKEIMIWQDPVPPVDYKL
                     VDANDIANLKGKILNSGLTTSELVKTAWASASTFRGTDMRGGANGARIRLAPQKDWPA
                     NDPQELAKVLKTLESIQNNFNNAQADGKKISLADLIVLGGNAAIEQAAKQAGYDIIVP
                     FSPGRTDATQEMTDVKSFEVLEPTADGFRNYFDKSNNMSPPEMLVEKASLLKLSVPEM
                     TVLVGGMRVLNANTGQNQYGVFTDKPGTLNNDFFINLLNMSTEWKKSSETEGIYEGYD
                     RKTGKLKWKATSVDLIFGANSELRAVAEAYATDDAKEKFIQDFVNAWVKVMTADRFDI
                     KAANANINS"
     gene            54872..>55382
                     /locus_tag="U6309_000209"
     CDS             54872..>55382
                     /locus_tag="U6309_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEN5503680.1"
                     /translation="MKNKLLPNLIIIALAATISANSITVQAKQNDQIQNSNEQLTQDP
                     FDTDPFFQSHDDVLLQIHKMQQAMDQLIKSQFSQMQNSLANDPHHNPFGSAKNIQITE
                     NKDELIYKIKLPQDSDSKVDITVKNGQLVLSANVTQKITREQDNNKSVSFSRSNYMQS
                     FPLPQSYDPK"
BASE COUNT        17209 a        11149 c        10656 g        16368 t
ORIGIN      
        1 ttgggtcagg atgttttttt atctcttaat attctcttaa agtttggcag ctataatatt
       61 tacccactgt gccaattttt aagtcacgaa tattaatttt aaagtattta tccaggagtt
      121 gtgtaatgcc taagtacgtt gaagggatag aattaaccca agaagggatg catgctattt
      181 ttgagcgcat gggacatcca aatattacca gcgggactat ctacaacgga gagcctacaa
      241 ttgataaagg tgctcttgac aggcaaggct ttatgccagt tttgaccggg gtcagcccaa
      301 ggcaagactc gggacattgg attatgttaa ttaaaggtca aggcaatcaa tatttcctct
      361 ttgacccgct gggagaaagc tcgggaaaat attaccaaaa tattttagct aaaaaactac
      421 ctggcgctac gctttctgtt attcctaata atgctggatt aaatatggga ttatgcggct
      481 attgggttgc ctcagttggt ttaagagctt atgcggcatt aacacaacct attccccctt
      541 ctttaagaaa tctaggtcaa accattaccc aggaaatgcg agatgaacta acacaagatg
      601 gaagcgaaaa aattacacaa tggttacgcg ccgttggcaa tgaatttcca gagggtgata
      661 tacaatctga tgccaccgcc ttaagacgtg ctactgaaaa gaatttacgc attgatgaac
      721 ttcagcctgt ttttgcagga acaacaccca aagaaatctc gataaatcct actgctcctc
      781 aagagacatc cgttcctgcc tggaatggtt tttccttata tactgatgaa atcgtgagaa
      841 atgcggccag atacgcgtat gataattatt tggggaaacc gtataccggt acagtagaag
      901 cgacacctgt caattttggc gggcaaatgg tgtacagaca acaccatggt cttgcccaca
      961 cattacgcac catggcctat gcggaaatca ttgttgagga agcgcgtaaa gcaaaattaa
     1021 ggggtgaatc attaaaaaca tttgctgatg gccgtactct ggcagatgtc acgcctgaag
     1081 aattgagaaa aatcatgatc gcccaggcat tctttgtcac tggaagagac gatgaagaat
     1141 cctccaaaaa ttatgaaaaa taccacgaac aaagccgaga tgctttttta aagtatgtcg
     1201 aagacaataa atctgcttta attcccgatg tattcaaaga tgaaaaagat gtcaaattct
     1261 atgctgatgt cattgaggat aaagatcata aatgggcaga ttctccagct catgtcctgg
     1321 tcaatcaggg ccatatggtt gatttagtca gagtaaagca accccctgaa tcttatctgg
     1381 aatactattt ttcgcaactt caaccttgga taggctcaaa agcagctgaa gccgtttttg
     1441 cggcacagag acaattcttc catgccactt atgaggcagt tgcaggtttt aatagcgaaa
     1501 acacagaacc tcatctggtg gttgatggct tagggcgcta tgtgattggg cctgatggta
     1561 atccaatacg cgaagaactt gatgacgaag acgaggaaga gtctggtgaa ttaaagtttt
     1621 tctcacaaaa aaagaaactc gaggaaaacc aacgttacat gcgcgttgat gaatatttaa
     1681 aacttgatga ggtacaaaag cgattccctg gcgctggcaa aaaactcgat gggggactgc
     1741 caggactgag tgaatatcaa tatttacagc gcttgaactc aataaacaga gcgcggtgtg
     1801 aaaacgatgt cgacttttgt ttggggcaac tacaaacagc tcatcatcaa accaaaatca
     1861 ctccaataaa gagagcattt caatcgagct ctgagaaagc gcgaagacaa cccaatatgg
     1921 atgaaattgc tgctgcacgt attgttcagc aaatcatggc caatcctgat tgcatccatg
     1981 acgatcatgt cttccttaac ggtcaaaaac tggaagaaaa attctttcgt gatttgttgg
     2041 cgaaatgtga catggctatt gtagggtcgc tattaaacga cacagacatt agaaatatcg
     2101 atacgttaat gcaacatgag agaaacacag aatttcactc aaccgatgcc aaagcaaaac
     2161 cggtgaaact gggagaaaca tgggaaaaaa ccatcagatc aggtggcggt gttacccaga
     2221 taaaacatga tttaattttc ctcatgcaaa atgatgcctg gtaccatact cgagtcaatg
     2281 cgattgcaca aaaccgagat aaagattcaa ccttcaaaga agtgttgatt accgctttaa
     2341 tgacaccatt gactaacaaa tcattgatag atacatcgcg ttctccagcc cccaaaacgc
     2401 tattccgtgg cttggatctg tcagaagagt ttaaaaataa attaattaac caggctgaaa
     2461 ccatcattgc caatacgaca gaacatctct ttaccgatct gtcgacagaa gcctttaaac
     2521 aaatcaaatt aaatgacttt agccatgttt ctgctagaac ttgtgccagt acttccacta
     2581 atattgaagt tcccaggaca atctttggct ccaatactat ctttgaaata cttgatcctg
     2641 atggtttatt acaccccaaa caagttggaa cccatgtatc agggtcagaa agtgaatatt
     2701 ccatttattt gccagaagat gttgccttgg ttccaatcaa agtaagcttt gacggaaaaa
     2761 cagttaaagg caaagaccga catattttta ctttagtcgc ggtaaaaagc cctgatttca
     2821 caccaaaaca tgaaattggc tatgcggttg gtcctctttt gagaatgcaa actccaaaat
     2881 tagaagaaat tcaaagatta gttgagcaag caagggaaga gcctgatctg gaaagagtat
     2941 tcaatttaca atcgagagta gcaagacaag ccaaattatt ctccactgaa tcaggatata
     3001 aaacgtttct gaatgaaaag gtggcgcctg tgttggaaca aagcctcaat ggcctcttgg
     3061 acaataatgt aaccattcta ggcaaagtat tatcagcatt cccttcagac gggcaatggt
     3121 ctgcctttaa ttcagttgaa gcaatgcaaa tgaaaataca gatggatgcc attaagcaaa
     3181 tggttgaaaa aaaagcagtt ctcgaaggtc gaattctccc tgccttggct caatgccagg
     3241 atgccttgga gaagcagaac atcgatggtg cattacaagc gcttaaaaat atcccctctg
     3301 aaaaagaaat gcaaactatg ctgtctatta gcagtggctt gagaggacaa atccagcgtg
     3361 caaagcaaaa tttgactgag aacctggaac cactacaacg tgccatgact gccaaattgg
     3421 taagcgatca agaaaaagtg aaagtgcgtt atgaaaagct gatagctggt atacctcaac
     3481 agatcactgc tcttgaaaaa gcagcgcttt ctgatcttgc cggtgttaaa aaagttgtct
     3541 ctcattttaa tcacctgcaa gaagaattaa agttgctgcg taatgaaaaa gttcgaatgc
     3601 acactggctc tgaaaaagtc gatttttcag atatcgcaca attagaagcg caactacaaa
     3661 aaatccatac taaattatat gacgcctatt tggttgaatt aaccaaagaa atttcagtac
     3721 tagctaaaga aaaaccgaaa aacctggccg acgtgaaaag gatggtttcg aatttctatg
     3781 ccatgtcagc tgatatggaa caattgcgtc aggaaaagat aaaaaaacat ggtgaatcaa
     3841 aagatccgat tgatatgtct gacattgata aattaaagga agaactacaa aaaattaatc
     3901 aatttttagt gaaagcgatg ggaactaata tcagggtttc aatgaatcaa atggaagtaa
     3961 aaacttttga tgcccaggaa aaggaagcgc aacaaaattt gaagcaacta gacgcactga
     4021 tcaataaatt ggagagttct gatgctgtac agaaacaaaa agaggaactc gaaaaattaa
     4081 accaattgct tgccgagaag cgaaaggcct atccggcaat ggtgcagttg caattcagga
     4141 gcgaagcatt aattatccat ttacgtgaat tatgtgaagc tcatcaagac catttggcta
     4201 aaacaagaaa aacaagagca caagaaatca ccaaaggacg atggaaagtg caatggttga
     4261 ctgattgggt tggtttaact tcagatgaac gagtaactct tgccaataaa gagaaagaat
     4321 tggctaaatt caaggaagat ttaaacaatg acgagtatga tttgcaggaa ctcatcagta
     4381 acctggctga gaaaaacccg tcagaattgg aagaagccat agggatatct aaagagagcg
     4441 ctcagaaatt gtataaactg ttaactcatc taaaccacag tacaacattt atgagtaaaa
     4501 tcgagcaacg attacagtct attgatgagc tattaaacga gtttggcaag caatcgcccc
     4561 gaactgaaat aataaaaact gctgaggaaa aacaaggaac tctcttaaga ttgtaattct
     4621 ttatcttcca cggtagccca ttaagggcta ccccgtggga tattttagat gattacggaa
     4681 aataagatta taattatcac ctcttgccgg atttaaaatg caaggatata tctaatcgct
     4741 gccatctcat ttatttttgc tcgcaatgcc ccatcacaac atttaccaca gtattagctc
     4801 ctaaaaaccg tatttttgcc ctatttttta tgagtgagag aaattgaaca aatagctctc
     4861 ttgtgctagg aatataaata tggaaagcgt tactatttct aaggatgctt atctgtgaag
     4921 aaaacaggaa gtgatgttct caaagaattt ataactacct ttgaaattaa ttatattttt
     4981 ggcaaccccg gcactacaga attgaaattt ctggaagcga ttgagcagtg tgataatgct
     5041 acctattttc tcacactaca agaatccagt accgtaggta tagccgcagg ctatgccatg
     5101 atcaccagga aaccagctct tattaattta cataattatg ttggattagc gaatgctgtc
     5161 tgtaatatgt acaatgcatt cacttcagga attcccatgc taataattgc tggccaacat
     5221 tcacgtcctt atttaattca tagcccagtc atgtccggag atctgacctc tttagctcac
     5281 actgcagtaa aatatgccta tgaggtgaat tcagccagcg atctggccgt tgctttacag
     5341 cgtggttatg ctcaggcaat tttgcctggc ccaggtccgg tttttctatc aatccctatg
     5401 gacatttggc aagaagaaac tcaagaaacc acgataaaga agacaagatt tttacatgct
     5461 tgcatcaacg ctgaaataga gcaaatttgt gaggttttaa caaatacccc caaaggaaaa
     5521 ttggcattta tagcggatta tgaggtaggt gcatcgaata gtatttgcct tttacatacc
     5581 attgccgatc gactaaatgc cgatatttat gctgccccct atcatgtgcg tgaagtaatt
     5641 aatcccttaa gtgtaaatta caaaggtaga ttgtctgata aatctggaga agtgcacaac
     5701 ctgctgctgc aatatgaaac tgtggtactt ttaggcgaaa aagtgaaagg gtatttatat
     5761 actggtcaat cttctattcc acagggtata caattcatac aaatcagtgc cgcatcggag
     5821 catctggcct ttgactatcc ttgcgatctg gcaattcttg gcaatcttga agcgaccttg
     5881 actgctatta gcaagcattt aaaaattaat ggggtaaatg tgccgcttaa accagccacc
     5941 aacattaaaa atttggaaaa aaaatataag caacatcctc tgcattcatt gattgttacc
     6001 ctcctgaatc aagtaaatcg ctctgttcat ttaataacag aaggatcatc ggaagatgct
     6061 gttgtgcagg acattgcaac aacactaggt tttttaaatg tgaatttttc acccagaggg
     6121 ggtggacttg gctgggctat gccattagca ataggtattt ctctagcgac cagacaccat
     6181 tcaatttgtt ttgtaggtga tgggggaagc atgtatgcga ttcattcaat atggtcagct
     6241 gccaaatata agattcctgt cattttcatt tgttttgtta atcgagaata cagagtatta
     6301 aaaacagata tgtctcaaaa tagatctttc acagaagaag atttttttcg cctggatttg
     6361 aatcatccac caattgatgt ccaacaaatt gctttatctt tcggggcaca ggttaagaca
     6421 attaaatcaa aagaagaagt agaggctgtt ctcaggagta gctttgcatt ccaaggccca
     6481 acatttatta ccctatatca ccaataaagc atttcgagag tcagacattg acttttgcat
     6541 tatctaaaaa tgtaaagcat aaaatcagtc tcagtattta aaaatttgtt attatacaac
     6601 tcataggaaa attattgccg ggaattaatt tttcaggtta gtcagattgc aatcagaaca
     6661 caagcctttg atttctaccg tacaattaat aattgaaaat tgaatggaat tcgccgcttc
     6721 tgttaccgaa gtaaaatcaa ctacacattc caattctttg acaaaatcac actgattaca
     6781 tataagaaat tgggcttgtc ccacatgatg cccatgcaaa caggcgacat aggatttcag
     6841 actgtcaatg caatgaataa atccctcttg atgccaaaat tgaatagccc ggtagaccgt
     6901 tggtggcttg ggattgtcta cttccataga taatctttgc aggatgtcat aagcaccgag
     6961 aggctttctc tcatcgacta atattttgag gacacgctcc cttggctctg taaatcgata
     7021 gccatggttt acacaatgtt tataagcctt atccaacaaa ttatgtttca ttaatcttct
     7081 ttcaagcgaa tagttagtgc gcactatttt atcataatcc ctgttgcaag gaaaaatgat
     7141 caaacaacag ccaatcatta tttcatagaa atcatttgct gtctaaaaat tatttaattt
     7201 ttcttgttat ctaacaatta aatgttacaa tataacattt tttgagaaag gcagtatgtg
     7261 cttgtaacgc ctggcttagg aagaaatctt attagggaca aacagctgga tctattgtgc
     7321 agatccagtt tcaaaatagg catttgcaga aaattaacct ttctctatag caaccagaaa
     7381 cccctttaaa tagaggaaag ccatgctgta tagtaattat cgattctacc ttgcaaaaga
     7441 tgcccaactt ctcaacccga aagaatccag agaagtaatt gagcagattt taactaaaac
     7501 taaagagttg gagttaataa gagtagctgt agatttgact agttccaata aaagcatcag
     7561 cttattgagc gcatccaaag agaatcatgt cataaaaacc aaagtgaccc agattttaaa
     7621 ggagcagggc ctttctgtaa tagacatgat ggaagtggaa gtgctccagg atattgaaaa
     7681 aatagagcat caccatccta ctcataagtc taagaacaca catcttcatc ccattaaaaa
     7741 gcaccaaaaa gcaaaacatg accattctca tgggcatggg caccaccacc atggacatca
     7801 tcatgaaaat cattggatca aagcaacatt aggtttgatt tggggaattg gattacttgt
     7861 tctttctatt gggactttta atattccctt ggttgcttac tatgtcatta caggacttac
     7921 ctctttgatg actttgtatt tgggccatac agtttatcaa tcagcctggc aagcattgaa
     7981 agaaaaaaaa tgggacacta ctactttata ctcaattagc actctaacta ttcttgctgt
     8041 ttctattgcg agcgtttttg tccctggcct acccatgatg tttgaggcag cccctctggt
     8101 tttaggcttt tggcatttag gtgaaggaat tgaacatact ctggtcggta aaatcaataa
     8161 aaaattggat gtccgggatt gtgtaccacc attagtcctg ttaaaaggta atccggataa
     8221 agagatttca gtcaagacat taattcctaa cgacatgatt atcgttaaaa aaggacaggt
     8281 cattcctgtt gatggtgtct taacgcaaaa agcgctcctt tataccacaa gaattgatgg
     8341 ctcgcccaaa ctcaaagaat ttcaccctgg cgatgttgta aaagctggca tgcaattggc
     8401 agatcatatt ccttctctgg aaatgcaggt taccaaaact tatcaaaatt cttatttgtc
     8461 attgattgcc gaaaacatta ataaagcgaa tgatgagaaa gcgcctatcg aaatgtttgc
     8521 agacaaggta ttgaaatatt ttattcctgg tttgattgcg gtagcgttcc tatcggggat
     8581 tgtgatcggt tcgttgttta acccagcact ggctattcaa tgcgtcgtgt ctgttctggt
     8641 aagtgcctgc ccgtgcgctt tgagtttaat taccccaatg gcagttaaaa tcggtatgaa
     8701 aaaagcctct gaaaacggca tccactttaa caacggtaaa gccttgcaag cagcagccga
     8761 catcgatacc gttgtttttg atttaaatgg cactctgact cagggcaaaa tggtggtgaa
     8821 atcattacat attaccgata aaaagtattt gcgccctatt gcattactgg aaagccagtc
     8881 ggatcatccg gtagcgaaaa ttattaaatc ttacatagaa cagcaagaca cggcttcaac
     8941 cgaaccatta gaaataacgt caattgataa gagtcatcat tccggcatga aaggtattat
     9001 taatggcgag gcctttatga tagggaataa agacatgctt gcagccaatg gaattacctc
     9061 tatcgacaaa ccctatgata atcctgaaaa aggttctgtt tatattgtgc gcgggacaac
     9121 ggtgataggt caaattgcct taattgatcc attgcgagag gatgccattg ccacagtgaa
     9181 acaattgaaa cgtcttggta aatccgtaca tatttgcacc ggggcagatc aggctactgc
     9241 agaaaaatat gcggcattat taggcattcc caaagataat atctgtgcga acactgtggg
     9301 ggctgtcacg aaaaaggggg aagtatccaa aaccagttat attcaacaat taaagcaaaa
     9361 aggctttaaa gtagctatgg ttggtgatgc cgctaatgac gctaccgcaa ttgcctattc
     9421 cgatataggc attgctgtta aatcaagtat cggcgactcc ataaccgaac aacatgccgg
     9481 catagtcgta caacaagggt tattattccc tatagcaaca gcatttgatg tagcccaaaa
     9541 aaccaaacag aatattttcc aaaacctgtt cgtgagccta agctataact caataattac
     9601 gcttgtggcc gcggggttgt ttgtggcatt aggttttgca ctaaatcctg tgctgggtgt
     9661 tgcattgatg gtacttgaat ccactattgt tttgacgaac ttatatcttt tcaagcaaca
     9721 agacattgtg tcctcaacat catcgaatgc caataatgct gtagaggtcg agcccgctgc
     9781 caatacaacc tcaaaagtcc tgcatgcttt agggcatcgc tcccagcctc aagaaaattt
     9841 agcaattttg acagagtcta aaccagtcaa tcccaaagta ataaatttgt ttgcgcctaa
     9901 agaacattct tcttataggt ctgatgtcac tcaacaaaaa ttggggtgtg gaatctgctg
     9961 atcaagaggt actgaaattg ccttgatttt ctacttcaag gcagttgtta cggtagtcat
    10021 cttttcatta ataactgttt ttttgaacta tactattatc aagaccatgc aaagcatcaa
    10081 gctataaaat taggatgatt caatgccatg aataacactt ggcatcaaac cgccactgga
    10141 aaccgctcca ttttaaataa tttgcgatta agcagaactc tacttaacgt catctttatt
    10201 attgcccttt ctttgctttt aatcatcaat attatttctt ataaccaggt caaaaattta
    10261 atcttcgcca gtaactgggt ttctcatact tatgaagtca tccaaaccat agatacatgc
    10321 ctttatgatg tagtagaaat tgaatctcgc cagagatttt acttgattcg aggttcaagc
    10381 cctcagctta tgactgatat tgatgatatg aaatcaacat tacaactgaa tttatacaaa
    10441 cttgagcaat taaccaggga caacccagaa caaaacaaac gaatccatcg ttttattgat
    10501 ttggttaaac aaagattaaa tttattaaac caacttgtgc agttaaaggg aagcggcaaa
    10561 ctcgatacac aagaaggtta tgatttactt aaccaaagtc aagacatgtc gcatcaagtt
    10621 aaaagtcttg gacaagaaat caaatccgtt gaattggtgt tgctatcaga aagaaatact
    10681 ggtgcggttc gctgggcaga taccagtagt tttattttaa tatccggagg tattgcaagt
    10741 atcctgtttt taacgatcgc ttttattctg gcaaatattg aattgtcaac acgcagaaaa
    10801 actgaactac agaatcaaaa tacgcaaata cggttaaaaa aaattataga aagtgccagt
    10861 gatatgatag cggcttttga caaagaccag cgatttatca cttttaatga agcttatcaa
    10921 cgtgagttta aacgtctttt tgataaatcc ataagcatca atatgtctct ggaagaagct
    10981 ttcaatgatg ttcctgaaaa caaaaaaaaa ttgatgcaaa cctggaaaga gtctctgcaa
    11041 agggatgaag aaacccaaaa tatcgaagtg aatacagaac aagaaaagac tatttatgaa
    11101 atgacctcca aactcatcca aaacggtgac aatgaaatta aaggtgtggt acacagcgtt
    11161 cgcaacatta ccaaacgggt acaagaacat actgaactac aagagtcgta tgaaaaactg
    11221 gctaatggaa tgaaagaact acaagagaaa aatgaacaaa tcaccctgct ggttgagatg
    11281 agtgacatca tgctggcttg cggctcccaa gaagagctaa gcgacgtaat ggctaaatac
    11341 tcccaacgac tactgcaatt ttcaagcggc tacctgttta tcatgcatcc ttccaaaaat
    11401 tatttggaaa aagcagccag ttggggtaat cctcaaccgc atgatctgac ttttaccccg
    11461 gaacaatgtt gggctatccg gcttggtcga attcattatg ccggttcatc gcgtatcgag
    11521 ttgatgtgta gtcatacgat gtttgccgag caacccgagt tgtctctgct ttgtgtaccc
    11581 ctaatggccc aaaatgacat ttacggcttg ttataccttg aagttggcct agagtttgat
    11641 gaaaaccaac aattactaat aaccgcattc gcagagctta ccgccttggc tttggcaaat
    11701 gtccgcctga gagaaaattt acgctatcaa tctatccgtg atccactcac tggattatac
    11761 aatcgccgtt atctggaaga ttttctattt aaacaattac atcaggctga acgaacaaaa
    11821 gcctcatttg ccattttaat gctggatttg gatcatttta aaaagatcaa cgataccttt
    11881 ggtcatgacg cgggagatct tgtattaaaa gagctggggc aaattttaaa cagtgatatt
    11941 cgcctcggtg atattgcagc ccgatatggg ggagaagaat ttgtcctgct gctctatgat
    12001 attgatgctc aagccgctaa aatgaaagcc gaaaatttgc gttcggccat ttccaattta
    12061 caggttaaat acggagcgca acctgttggg cagataacgg cctcaatcgg catatccgtc
    12121 tatcctgacg atgccaaatc acctgctgaa gtgattgaag cagctgataa ggctttatac
    12181 caagccaaaa acaaaggcag gaataaggta attttattct cggaaatatc atcctgattt
    12241 ggactgcgac tcccaaccta aatgttgtag tgtttcttat gtccaataga gttccatgtt
    12301 ttatatgagt tttgagagcg aaacaataga tactacactc tataacatcc ggtattttta
    12361 atagcaaccc agcctacgat aatatataat gcgcagccat caggcataat tactcgtatc
    12421 catgctctat caaggaatta tctcgaatgt ttagcaaagc cctaatcagt gcaacctata
    12481 aaaatggaaa acccggccaa cttacagaag aacatgaaaa agcagagcat caccgcttta
    12541 agacagaata tctgtttaaa aatgcagaga tccctaaaga cttatacgcc tcacgcttga
    12601 tccagcattt tttgattatt aaagccatag aaacaaaatt acaaaatttg ccaaaaacag
    12661 aacagtcaga aatcagcgca ttttttgctt tatcttatct tgagcactta tggcgtactc
    12721 ctgctataga aaatgattta cgacaactaa acattaatcc tgatgaaatt cataaggatg
    12781 agataacaaa aacaaccgaa aattatcttg aaaacataga aaaatttaca cccaaaatcc
    12841 ttctcgctca ttttttatta catattgccg gctttatgca tggcgggaat attatccgct
    12901 cgaaatacat tgaaccaagc aatcgcttga ccacctacca aataacagca gatcaatacg
    12961 atttttctgc tgccatctcc tgtttatcct cgggaaaaca ttcaccgctc gctctttatc
    13021 aggatatgat gaaacaaatc gataacattg ttctaactgg tgatgagtat caagagcttc
    13081 ttgagcaatg caaaggcatc tatgaaacca tgagcaatat ctacgatgat ttatgtgata
    13141 tgcacactca tcacctgaag ctgcccactt actcacttgc ggctatcagc gtcagtctgg
    13201 ttgcacttgg attaattcta aaactattga tggatttctt aaatccaatg acgaatccta
    13261 taacaagcgc ccctcgttaa ggcccatcgt cagaatgtta ttgtggcctg gatattaaga
    13321 tttacaatga tttatttctt tatatataat cattacccac ttttatagaa atttgaacaa
    13381 tatcgaacag atagtgttgt tttgacaaac aaatggaaca gtaacagatg ttaaaacggg
    13441 atatttcaac cctgaatata ttagttgcat cagctggtgg aatgattggt tcaggttggt
    13501 tgttcagccc atttattagt gcccaaatgg caggcagtaa ttctctgata agctgggtta
    13561 ttgcagcaat atttatgttg tttattgccc taccgctttg tgaactggga acgatgtttc
    13621 cagtttccgg gggtatgtct aattatccta cttacacaca tggtcgagaa gtgggttttt
    13681 tattcgcatg gacatcctgg ttatcttatg tcgttatgac acctattgag atacaagcaa
    13741 ttttgcaata ttcaagccat ttttttccaa tattaatcga caatcatagc gaatcactca
    13801 cactgtccaa ttatggttat ctcgctgcaa ttgggattat gttcttcgta gttattttga
    13861 attcttatgg gattaagttt ttagccgagt gtaacaagta tgccagcatc attaaatttg
    13921 ttttgccgag tatcgctatt atctcactgt taaagcaatc accctctctt gctaatgtta
    13981 atattaatct gtcaagcaaa gaacactggg tagagatatt taccgctctt tctgcaggcg
    14041 gagtagcttt tgcttttaca gggtttcaaa atggtttaat cctggcagga gaagttaaaa
    14101 atccacaacg aaatattcct attgccatac ttggcgcggt attggttggc tttattcttt
    14161 attttatgtt gcagttcagt tttatagcgg cggtaccaca aaagtatttg gctcaaggtt
    14221 ggcacgcatt gagttacccc ggtgatgagg gcccattggt tggattaact cttttgttag
    14281 gacttggcat tgttgctact ctgttattgt ttgacgccgc tttttctcct tttggcacga
    14341 ctttagttta taccgcagct acatctcgta ttttgtatgg catggcatta aaccaacatt
    14401 tacccaagtt tttcttaaag gtcaaccgcc ataacatacc ttacgtaacg ctttatgcaa
    14461 atcttttggt tggttctttt gcattcttac cctttccggg atggcaaaaa ctagtggcat
    14521 tcctttcatc agcaagtatt ttatcctacg gaattggccc gatttgtctc ttggccatgc
    14581 gtaaaatgca cccccatatg gatagaccat tcaagctcgc aaatagtatt ttcttgtccc
    14641 atatcgcctt ttatatatgt aatctcatgt tgtactggtg tggtttttct attatttgga
    14701 agctaaactt agcattgctt atggggcttg gtatttattt tttttaccat cgccatattt
    14761 ctttgagaaa tggctcgcct ctggtgtggt ttatcatgta catgggatca ttttctatta
    14821 tttcttattt aggatctttt ggcggcattg gtttattaaa attccctttc gatttaattt
    14881 gcttcttgcc ttatagcatt ttaatacttt acatgtctca agttttagtg acaccccgtc
    14941 atgacaatga catggttagt aatgttacag aattgccagt atacaattct tgaggtagca
    15001 aaacgacaaa ataaaaacgc ttcctgattg tccttgtctg ttttgtagtg aaatgtcatg
    15061 acttcttatg ggtaaaatgc atcaagagta acttgcctgc ttcctgccaa ggtaccctga
    15121 tgcatattga gtttccaaca ttttttacga taaagtgatt gcatcagtat aagattcgtc
    15181 taagttccgc ttcctgtaat cgagttgcca aagactcatc agactccaaa ccggtttgca
    15241 ctctgccaga aacgtgagta ttccttttat tctgtccatg aggaaagaaa ccataaggcg
    15301 ataaagtatc aaacagcgtt tcaaagtgtt ggtggtaacg ttttgttctc tctgattgag
    15361 tttgggtttt gaaggttctt tcaagaactt gctcttcttt caaagaaacg gctctgtcag
    15421 ataaggcttc ataaccaaaa gctcgcttac ggggtgtgta ttgtctgggt ccctggagag
    15481 attctacttg tgttatccaa tgtgtattac tcaggttctt tagagtgacc gtaggatatt
    15541 ccggaatagg tttgccattc ggttgtccaa caacattgag attgatttta agtaggtcgg
    15601 caatttcttt caaatcatca tgcgttgccc atcgtccttg ttctttaatt ttttcaaggt
    15661 tcttcaaaat aattgaattt gaattgattg tattgcctga tagaacatcg tttagtaaga
    15721 cttctttaac aaaagcattt tctattttct gtacctgatg aaaaggctgg tttctgagga
    15781 ttggtctcag tattttggca atatctcgcg ctgtttgaac aatttccgga gagagggcta
    15841 gttcattgaa ttgacttatc ctttccagcg aatctcttcg atagagataa agttgtacca
    15901 attccatgaa tttgcaataa gcatttgatc cttctatttt ggtttgatct ttacttcgac
    15961 cttcttcgat tattctcttg accaaatctt ctttattcac aatgactgca atatttcgta
    16021 aagacatctg caatagaaat aataattcac ttttataagt gtagggtgaa tcatgaagtt
    16081 ttgttagatc tacattaagg atgtcttgca gtcgtactcc ataaatacct tcgacttgct
    16141 tccatcgatc ataagtttta ctttttccgt aaagtttgta ttcattttgt atgatgctta
    16201 ttaaaccaat agcaagagca taaaatccac aatccccgcc accaggatta tctattgaat
    16261 atgacatctg tacactccct tttaggatta atgatttaat gtttcagtca agcattataa
    16321 aatatgaaca ttaagtaaac attatgtaac atatgccaag ggggagtgca agtagctaac
    16381 aattatcttt tgcctcgcct gtgggtaagc aagctattta tcaattgggt ctccaattga
    16441 tatccgttat tattgtgtgg aagaaaaaaa tctcgctaaa gccgtagcct cttgtaaatt
    16501 gacttctgca ttaatccaat ttaacttgac ttgcgctgtt gtttgttcac gttgattcac
    16561 taaaaaaagc gtgctatcac cttcataaaa tctcttggtt tcccccttct ctacttgccg
    16621 tgcaagattt aactctttat taagcaaaac cacttgctgc tgatatattc ttattgcaac
    16681 caagagattg gacagctcgt tatttaactg ttcgtaggta aacttcattt ccgtttttac
    16741 ttgctgtagt tcgctcgtgg tgctgataag ctttcctctt gcttcacgtt gaaaaatagg
    16801 aaatttaaaa ttcacgccga tcatagcagc ctgagggatc aataaaggat aaccgcctgt
    16861 tccatattgt ttaaaggtat aagctgtcgc atcaagatta ggaagaagct catttcttgc
    16921 taaattttgt tttaatttaa caatttttga ataattctcc aatttacgca aaacaggatg
    16981 ccgttgtaac tgtgaaatac tattggatac tcgaattggt tgggcaggaa atgttgccgg
    17041 taattgactt tcatccggtt tttgcggttt accattcgcg tcacgatagt aaagtgaaag
    17101 attgatacca gcttgcgcca ataacatttc tccttgattg accagttgtt cacgttgaac
    17161 aataagttgt aaattttctg caatggcaag tctaggcaaa tcaccttgtt gagcttgttg
    17221 aataatcgca ttttgccgct ctttcgctaa atttagcaaa tgcctgaaag ttcttaactg
    17281 gaaacccgcc tcaacccatt gccaataggc tttaatggtt tcctgataaa ttttaatttt
    17341 tgtggcggca gcatcctgtt gtttcatgcg aattgtttct tctcttgtaa aaagctcggt
    17401 acgctcttta tcaattcgtc tgtctctcaa caaaggtaag gaaaggcctg cacgatactc
    17461 accaccagag ttagtcaggt aattttgata gtaaatgggc caatcacctc taccaatacg
    17521 atagcctcca aacaatttaa gtccattata gagggtgggc acattaaaaa gattatctgc
    17581 ataattatta atataccccc caacgggctg gaaacgagga ttcacatcga aagaggggtc
    17641 aaattttcct aatgcattga cataatcacc ttccgctttt ctgatttcaa gacgcgctat
    17701 gactatctga gggtaacttc gatccacact tttaagtacg gcatcaagca gcaaaggttt
    17761 gcctggtatt ggaaaacact tgaatgacat catcaagaac aaacataaga caatcaatct
    17821 gtttttgcct gaaggaaaac aaatcatgat gctttattct cgcgatcact ttccggcgga
    17881 aaaccattat actgacgcca aagctcatac cataatggaa cttgacccaa ttggatccac
    17941 ccatgcaccc gcactccttg ccgtaaaaaa cgcacactag gccatggttc attaggaaca
    18001 atcactacac ggaataaacc aaacccatta tctgtcggat caataaagga gactgtacct
    18061 ccaaatgttc cgactgcaat ttctggccat ccgcgaaatt gaatagcagg ccatccttca
    18121 aattgcaacc gggctttttg attaaggcgc acgaaaggta tatcattccc gtcaacccat
    18181 aattcaacgg cgcgagactc tgtttcaggc agaatttgag ccaatacatc gcctgcgttc
    18241 acaacgacac tttcctgacc tgtcaaacgt ctaaagatga tacctgcaac atgggcttta
    18301 atgatttgtg ttttttgacg agcaattcga acatcgacat tcactttgtc aatattcgcc
    18361 tgcgcaagtt cattttggta ttttgcatat tcaatttgtg ccaattcata ttgccttctc
    18421 gaattaatcc cctgttcata caatttcttt tgtctgtcaa cattggcttt acctgcagca
    18481 atagcttgtt cggctgcttc tatacgaagc aaaatcgcct ttttctcaac ttcaagacgt
    18541 gacaaaagtt ccggatcatt atcggtaata tcgacaattg gatcacctgg tttcacacgc
    18601 atgccttcgt cgacatacca tttctttatt cgtccgctaa taggtgaatt aaccgtatgt
    18661 tgcctctctg ttggtgaaaa ggcgatgact tttccattac ctaatgcaaa ctgttgccac
    18721 ggagtaaaac ttaagaaaag aataatagca acaaaaacaa tgaaaattat tttccctatt
    18781 ttttgaggct tgggcagttt actaatcata gaataagcat gtaatttcat gaggaaatca
    18841 ccaggcgatt atttaaatag ttaaaatcgg atcgttggct cacgatcact agcagcgtat
    18901 tttttaaaga caataccaat tccatcattt cattcacttc ttgatgatta aggatgtcaa
    18961 aagcgcaatc gataacgatt aactctggtt gtaatagaat agccctgact aacatcaatt
    19021 tcgtgagctc aactgaggta aatacatcct gccattcata gagaatagtg cgtaaacctt
    19081 caggtagttg tatgatttta tcagcaagtc caacattctt taattgttca ataatgattt
    19141 tgttaggcac gttgcgatga ttaagtacca agttgtcata aatgcttccg gtaaaccact
    19201 ggggctctgc aatcaataaa ctaaaacgac gaagagaaat aaggttcttt ctcgcacaag
    19261 gaatctcatt aataagaaca tgaagtgagg caggatcttg gaatccaaat agctgctcaa
    19321 caaacaattg gcataaatcc gatttttccg aaaagaccaa taaaggttta tcaactgtta
    19381 tcaaggcctt gttcttgcta tctatctcta actggatgct attaattgga gtcaataact
    19441 caccatgctc atcttctttc acctgttcaa gtgacagatt aagtaccgca tcaattttat
    19501 tttcagaggc gaccagatca taataatttt ctaaaagggc accaaagcgt ctaaacgagt
    19561 aaatcagagc acctaaaacg atttctgcgg caaccaattg tccaagactt aactgattat
    19621 taataacaag atagcccccc aatcccaata aaagactgct ggccaaagca gacagcgtgt
    19681 aaaaaccaat ttgatgtttg ataagctgct taaaatgggt atttcttgct tttaggaaag
    19741 caaccaattt tttatccgtt tgttgagtca catagcgatg atataaacta aaacggaaca
    19801 gataccggtt aatcaaaatt tcttctaacc aagcacctac aacatgtttt tcagaacatt
    19861 cttttttggc gctgtctaat gcctttttgt aagggataaa aatgatcagt agaatgccaa
    19921 gaatgatgaa aatatcaaac aaaaggaaaa gaggatgata gattagaatc agcaatagtc
    19981 caaaaaaaag ttgtaacccc aaattaatgc catataacaa taaactcgcg agtgattttt
    20041 tgatagtgac tatttcaaaa aagcgattga ccaactccgg tccatgatgt gtcgaaaaat
    20101 tatcaagaga catgtgggtg aaatgtcgtg tcagacttaa actgacttta accatcagtt
    20161 tttgctgaat tacttcaata atgacaattt gccatatgtg gagagcgcct agagccatca
    20221 ttaaaatcaa taccatcaaa cttaacgtaa tcacgggttg caatagcttg ccaaaagcaa
    20281 ttaaattcac gagtgtttgg gcagcaatgg ggatactcaa tgacagtaag ctgataatta
    20341 cactaatcat cgcgatggat gagattgttt ggcgatctaa tacatcaata attgaattaa
    20401 ttggttttgc attcattccc taaacatccc ttttaccctt gattcttgtt aatgctatta
    20461 atattttaat ttttgcaaga taaaaaattc cagcgattca atcgctttat tgctttcact
    20521 attttttaat aaagaaacat ggacatcatt caatataggg agaaaatcgt aatcgagccg
    20581 ttccagatag tttgggatca tgctgcgctg aatagcagta atccctaatc ccgcccttac
    20641 agcagccatt tttcccgcat aacttgcact actgtataca aggcgccagt tgatgtgagc
    20701 tttatccaaa gcatcaatga catttccccg ataaacacaa ggtgttggtg aaagcactaa
    20761 agggattggc cctggtttgt ctaaaagagg tagcaattct tttttgccta tccactcaac
    20821 aggttctatg cacacttcaa tacactcagt accaatctgt tgctgagttg ttttaataag
    20881 aattaaatca aatttacctt cattaaaacg ctcaatgaga ttaagcgtca agtcacattc
    20941 cacattcaat agcacacgag gatgtaatct tgaaaattcc acgagaacat ctgataaaat
    21001 aatgctggca aaatcctctg gcaatccgaa gcggatttca ccttgtaact caggcgcttt
    21061 aaaacgatcc aaagactctc gatgcaactc ataaattcgt ttggcataac ctaaaaatat
    21121 ttcaccatcc gtagtgagag aaagttcccg gccacgatta atgagaggtt tttcaatcag
    21181 gttttccaat ttagctattt gctgactgat agccgattgt gtgcgtccaa cccgctgtgc
    21241 tgccttggta aaactttgcg tttcagccac ggcgagaaag cattgtagtg tgattgtatc
    21301 aattgacata agtttttcta atagtttaga taattatttt taattttact tatattaaca
    21361 tatctaatat atttccacaa taagaacaga atttctaatg gagaactaat gtggataaac
    21421 ttttaaatgg gctatcgatt tcactcatgc tatcgccctt aatagcgatg ctcttctata
    21481 ccttgttagg aaagacaaga aatattcaag caacttatta cggtaccaca gtcataggtt
    21541 ttggctttgg attgagtctc ttggcattaa tgatttctgc aaatactgta aaccaaaaca
    21601 gctatttttt agttcatgct agcacactaa gcctcttgct aaccacatta atattattca
    21661 tcagctttat tatccatcgt ttttccatac gatacatgca tggggataag tgctaccgac
    21721 gttatttttt caaactttca gccataactt taacaactgt tgtgatggct ttagcagaca
    21781 atcttttatt attttggctt gcctggtcgg taagtaactt gctcttaata aatcttatga
    21841 ttcacaagtc gcaatggaat gctgcttaca actcaggaat attgacttta aaaacactat
    21901 taccaggaag cttatctctg atcacagcat tttgcatatt ttttgcacta aataatactc
    21961 tgtcgattga aataataacc agcgaagtga gcaataagcc atcccctgct attgctgtgg
    22021 cgcttggatt tattatctta acagcgatga cacaatctgc catatggcct tttcatcgct
    22081 ggctaatgag ttcattaaac tctccaacac cggtatccgc tctaatgcat gctggattag
    22141 ttaatggagg gggctttctg attgttaaat ttgcaccttt gtttgcaacg caagcaaatt
    22201 tattaaccat tttattcgtt ttcggtgcac tgactgcttt attaggcacc ctctggaaac
    22261 tattgcagtg gaatatcaaa agtatgcttg ccagttccac catggcacaa atgggtttca
    22321 tgatgatgca atgtggctta ggacttttcc cagccgctat cgcccatcta tgctggcatg
    22381 gcttgtttaa atcctatctt ttcctcaatt caggatctgc catccaacaa aagaaatatc
    22441 aaaataaatc aggtcccgaa aaactgacga ctttattgat tgcatttttt ggcggactgt
    22501 tgggaatgta tggttttgcc ctaatcacag gcaaacccat cttatcgctc cagacatcca
    22561 cctttctttt aggttttgca ttcatcacag ggacacaagt caccatgagc ttgattgcaa
    22621 atgagaagca tttcaaaaag ggaattttag ccttagcttt agttttctca attggagttt
    22681 tttatggagg aagtatccat attatcgagt cacttttacc taatccaaca ggatttcctc
    22741 aacaccaatt aagcagctta caaggcttaa ctctgattat cttcttttcc ctttggattg
    22801 tattcagcat ggggttattc aaaaaaatca gagagacaag actttgggat tggttttaca
    22861 tgtacatgtt taacgcgagt actcctcatc ccaaaaccat tacggcaaat cgccatactt
    22921 attactatta agaaaaggag caaacatgag cagccacatc actctaatca aaactaataa
    22981 ggacaatcaa aatcaaatga ctcaatgtaa ctacccaatc gataatgatg tcatgattat
    23041 ccaggccatg gtaaataatg ttgcaaaaca aattacacct gtatggcctc tggaaaaatt
    23101 tattgcctgt aattccttgc atggttttga atcgatgtcc tttgaagagg ctatcctgat
    23161 aaatcaaaca gcaaaaaaag gcactccatt caatgaaaaa ctggaaagag ttaattggca
    23221 catgatcaaa tggtgtggga gctttctgga cattggacaa ggcacgattg aaatgcctca
    23281 tcgtgataaa ggcttatatt ttggcttttt aaagctagcc ccttttgaca gcgcactgca
    23341 tcaaaacaat aaatcaacaa agagctggtt atcaaacctg ccagaaatgc ctgaacaagc
    23401 tatccgactt tgcctcgata aacttggcgt attaaaacaa agacaagagg aatttctcca
    23461 atctactctg tctcacttac ctggatgggc tggatacatt aagtggataa gcgaatggaa
    23521 aaatcgtaat ggcaaggaag aaaatcctgt aagccttgtc gattttcttg cagtgaggct
    23581 gataatcacc tgcgtattat ggccagaagc aaatctggaa gaaaaaaaaa aggaaaaaga
    23641 tagtacgcat actaaacaac ttattcaaaa tataaaacac aaagaagatg attacaggca
    23701 attgctttta aagaagcttt tgcctgagtt aaacaaagtt cagataaaag aaaatagagc
    23761 taatgcccaa atggtttttt gcattgatgt ccgttcagaa ccgtttcggc gatgtataga
    23821 aaaactgggg aactatgaga ctttgggatt tgcaggattc tttggtttac ctgtgagtat
    23881 caaagattat gacggtgaaa caataaaaga ctcctgccca gtcctcttaa agccacgatt
    23941 taatatccat gaaaaagcaa ttgctgcaaa tgaacattgc atagagcatc atgagaaagg
    24001 taaagagatt aaaaaaattt tgaaccgggt ctatcagcaa ttaaagtaca atttttcaac
    24061 cccatttgcc ctggtagaat cattaggaat ctggtgtggg attacaatgt tcctcaaatc
    24121 atgtagccca attttcgcta gacgtattac caaaaaccta aacgaaatga tatgcccctc
    24181 cattcaaaca cagcctgttt ttgaattgga tttattagag aaggaggtgg ggatctcact
    24241 tcaagaacaa attgcctatg ccgaaatggc attgagactc atgggactga ctgataactt
    24301 tgccaaactt gttattttct gtggtcatgg cagctctacg caaaataatc cttatgcttc
    24361 agccctggat tgcggcgctt gtggaggtaa tcaaggtgga aaaaatgcgc aattactggc
    24421 gtctatcctc aataaaataa cagtgagaag ggctctggca gaaaatggaa tcaatattcc
    24481 tcaagatact gtgttctgtg gcgcacaaca tgacacgacg acggatgagg ttgaaattta
    24541 tcattcaaat gtcagtcaat tcattgacca agatatcctc gaccagctta gaactgactt
    24601 aaaaatggct aagcacaata ataatttaga gcgaattaac tatttgaatt ccatagattg
    24661 cccggaaaaa gacattgtac gaagaagcac ggattggtca gaaacaagac cggaatgggg
    24721 acttgctcgc aatgccgctt tcatcgtagc acctcgccag ctgaccaaaa acattgatct
    24781 ggaagggcgt tgtttcttgc attcttatga ctggaccaaa gatgaagacg gcaccctgct
    24841 tgaaaccatt ttgactgcgc ctatggtggt tgcccaatgg attaataccc aatacctgtt
    24901 ttcaacaatc gacaacgtgg catatggaag tggcagtaaa atcactcata atgtcgcagg
    24961 aaaaattgga gtcatgcaag gcaatgccag tgatttaatg catggattgc cactgcaatc
    25021 tgtcatgagc tatgatgaca aatcctttca tgagccacaa cgccttttaa ctatagtcta
    25081 tgctcctcgc gaaattatca gtgaactagt tgaaaaacac gatgtcttaa aaaccttgtt
    25141 ttttaatgaa tgggttcatt tggtagcgat tgatccacgg agtcacttgt tttacaagtt
    25201 agagaaaacc aatacctggt ctgtaattca gtgaaaggag tttcctgatg aatataatca
    25261 aagtatcaga aaaaaaagag atgaacatct ctttgcatcc gatgaaaaag atcgaagtga
    25321 tcgtatctgg tgaaaatgaa aatatggtcg ctaacatgat ggagaaggca aatatttctg
    25381 gttttacctt attgcgaaac gtctctggaa aaggacatca tgggtttcat gaaggtaaaa
    25441 ttttgtttaa cgacaaagcc tctttcgtca tgtttattgc agtggcacct gaggaagcta
    25501 ttgccaaaat tgcacttggc atgaaaactc tattccaaaa gaattcagga gtcctctttg
    25561 tgtcggatgt ttctgttgct agagtcgatt atttctctta gtatttaact ttggaggtga
    25621 cccttatgca ctatcaaaat cagcgtgttt tactggcctc aaaacatcaa aaggaaaaag
    25681 cgattcagcc tccttttgag gagaaattag gctgttttat ccatgtccct gctgattatg
    25741 atactgatca gttcggcacc ttcaccggtg aaataccaag aaaagcctca gcctatgaaa
    25801 cattaattct gaaagctaaa gcagcagccc agcaatttgg ataccgttat gttatatcca
    25861 gtgaaggaag cttcggcccc catcctcagc tttatttttg tcctgcagat actgaattga
    25921 tggcctttgt tgatttggat aatgacttaa ttatcgctga gcacgaatta tctacagaga
    25981 caaattacag tcatattgat atcagtgcca cagatgacta tgaagcgttt ctcagcaaag
    26041 caaaatttcc aagtcacggt attattgttc gagccttaaa tagcgaagaa aattatattc
    26101 aaaaaggaat ctgtgagctt gagcaactta aaacagcgat tcaaaaagcg ttcacataca
    26161 gtacaattat ccgccttgaa accgatatga gagcgatgat gaatccactt cggatgaata
    26221 taattaacaa acttgcggtg aaactggttc agcgcattca gcaacattgt cctcaatgtc
    26281 acactccggg gtttggaaaa atctcggctg aaggtcattt agcctgcatt tcttgtggaa
    26341 caccaacaga actttacctg cgaaaagttt taaattgtct aaaatgtgag tttaagactt
    26401 atcaagcgcg agacgatgga ctagaatttg ctgaccaaag gtattgtcct tattgtaatc
    26461 cttaaccgtt tgcgagaatc aagtctatgc tatgttataa acattcaaac aagagaggca
    26521 cactatgaaa cccatattaa taggtctaat tatgggttct caaagtgatt ggcaaacatt
    26581 aatccatgcc gcccaaacac tggatgcgct caacattggc tatgaggcag aaattgtctc
    26641 cgctcatagg acacccgaca aacttttcca gtatgcggaa caggctgagg aaagaggttt
    26701 ggaagtgatt attgctggtg caggaggagc ggctcatctc ccaggcatgg tagcagccaa
    26761 aacatctttg cctgttttag gtgttcccgt catgtcacaa actttaaacg gcgtcgattc
    26821 cttgctatcc attgtgcaaa tgcctgcggg gatacctgta gggactttat cgataggtaa
    26881 agcaggcgcc attaattctg cactgtttgc agctgcaatt cttgctaata aatatcctga
    26941 tattcgagct gccttaaaac gctatcgaga acaacaaaca caaaaagtgc tcgacaatcc
    27001 aaaccctaaa gaggaaaagt catgaaactt ggtattctag gtggcggcca acttgcaaga
    27061 atgttgacat tagcagcata tcccttaggg attaacactg tctgcctgga tcccagccct
    27121 gacgcctgtg ctggtcaact caccaaactg atagtctctg attttaatga tgaattggct
    27181 ttaaaacaat ttttgtcaga agtggattgc gtcacaatag aaaccgaaaa tatacctttt
    27241 tcttgcgccg aattggctgc ccaaacaaag cctttctatc catcaaccca agctttaaaa
    27301 attacgcaag acagattgta tgaaaaaaat tttttcagaa ctctgaatac tcctgtacca
    27361 gaatttttac cggttgaatc agaagatgaa ttaatgcagg ctatttcagt cataggaatg
    27421 cctgccgtgt taaaaacaag aaggtttggc tatgatggca agggccaata tgtcttgcga
    27481 acccaagctg atatttcaaa aagttggagc ctgttaggca gccagcctct cattcttgaa
    27541 aaattaatct cctttgaaag cgaattttcg ttaattgctg ttcgtaataa gagtggtgaa
    27601 acaagtttct acccattgat taaaaatcat cacaggaagg gcattttaca tttctcagaa
    27661 gcccctgttc atcatgatac cttacaacag gaggcgcaaa ttcatgcatt caaaatttta
    27721 gaagccctga attatgtcgg tgtacttacc attgagtttt tttatcaagg caaccaattg
    27781 attgcgaatg agatagcacc acgcgtccat aattctggcc actggactat cgagggagca
    27841 cataccagcc aattcgaaaa tcatctgcgc gctctttttg atttgccctt gggaagcact
    27901 gaagtaagcg gctcttgttt tttattaaat tgcattggca atctgctgcc attagaaccc
    27961 tgtttatcga tcccgggcgt tcattaccac gcctatggaa aagcaccccg gccagatcgc
    28021 aaggtaggcc atgttaccct tgtcgatact catttggaac gttatacaaa aagtaaagac
    28081 ttacttcttg acatttcatg agtagcagat agtgagaaag cgagcaatgg cagtcagtgc
    28141 ataatattgt tcgcaatttg gaaagctgag ctttgtattc tgtttcctac tgctcatcca
    28201 cattatcctg cttattatta gttgtcacta tagactcggg atcaatggag cttccaggaa
    28261 tttttcggct ttcgctggcc cattctccca gatctattaa cttgcatcgt tcagagcaaa
    28321 aaggcctgaa ctggttatca gggctccagg tattttgctt gccacaaatg gggcatttga
    28381 ttttttgctg gttgttcatt aaggttttgc taatttatat agtttagata tgaagtatgg
    28441 ctacagcagc aatgattttc aatacacctg cctcatttgg acagtaggga aaatagatct
    28501 cgtgcattcc ccaatctaaa actaattacc aacatgcact tcgcgcacca gagccaacca
    28561 agcttttgca gcctcggaga gataagaatt tctacgccaa gtcatcgcca tattccattg
    28621 gaactcactt ccattaagta aaagagagcg aatttgtgtc gtatgccttt gagtggcaat
    28681 catccgtggc aaaaaagtaa ccccaaggcc tgctgcaaca agctccagca taaaatcaat
    28741 ctgactgctt tgagccacca ccttaggttc gaaaccagcg tgacggcacg catccaatat
    28801 aattcgatgt agtgcgaaac cactgccaaa aaggatgaaa ggtatttctt taagttctgg
    28861 tagagaaatg caatgtttag ttgctaatgg atgtccagat gccaggagcg cgaccagcgg
    28921 ttcagagcgt actaattggc aatcaaattc ttcggaaatg ggtaataagg ttccagcaaa
    28981 atctatctct cctgcacgca aacactctgc aagtttatcg ctgccatgtt caacaagctc
    29041 tacttcaatg cctggataac gttgtctgta cagaacaaaa agaggggcaa ataaagtact
    29101 gctacccacc ggagaaatac ccaaccgcag tcgaccttgt ttcaaacctc ggatttcatc
    29161 caattcctta agaagatcat ctcgatcagc caataatttt gttcctcgct gataaacaat
    29221 ttcacctgcg gttgttggta cattacgctt tttaaaccgc tcaatcagag tgatacctaa
    29281 ctcctcttca agctgtttta tggctttact cacggtagac tgggtcgcaa agagattttc
    29341 agctgctttg gaaaaactac cctgccgtac aacctctatg aaagtctgaa gcagtttaaa
    29401 ttccataatt attccatatt ggaaagatta ttagtctata aattcatttt attcatctta
    29461 attaaaagtg taaacttaaa tgaaaaaata atatccacca tgagcacata tatggcaaac
    29521 aaaattcagc aaaattcatt ttttcagata gtattggttg gcttattttg gttagctagt
    29581 gagttattgc tcaaactggt caagcttcct ttgtccggag gaatccttgg cttgggaatt
    29641 gttttactgc ttttggcgac gaattgctta aaattaaatc gtataagacg gggggctgag
    29701 ttactacttg ccaatatgtt actttttttt atccctggcg tccttgcaat actggagcac
    29761 cgggaattta tcggtctttt aggccttaaa attctcttca ttattctgct cagcacaatc
    29821 acggtgatgc tagtcactgc ccttgttgta gattattgct accgttggag aacgcatcat
    29881 gccaagcagc attctctctg aatccatgat tcaggcattt ttctggtcgc ttatcacaat
    29941 aggcacgtat tttattaccc aaaaactata tagaagatgg cccttttggt ggctaatgcc
    30001 gcttgcttta acaccaatac tggttgccag cgtagtgctg ttactgcatg taaactacca
    30061 ggactatttc cgcggaacaa aatggctagt cttgttaatt ggtcctgcaa ccgtggcctt
    30121 tgccattccc atttatgaaa aacgtgccct cattcgccag cattggccag tacttttaat
    30181 tggcgtgatt gtgggaagtt tcactgccat agcatccagt tggggattgg caatccttct
    30241 tggccttgat gataatttac gtttaagtct gctccctcgc tctatcagca caccgtttgc
    30301 tatagaaatc tcaaggggaa ttggtgcagc gcctgatttg actgcgattt ttgttattgt
    30361 cacagggata ttgggagcta tactcggcga gttcatcctg tcgcgcttgc cttttaactc
    30421 agctcttgcc agaggtgccc ttttaggagt aggagcccat gcagcaggaa ccgctcaggc
    30481 ccataaaata gggaatacag aaggcgccat tgccagtttg gctatgattt tagtaggtct
    30541 attcaatata cttcttctcc cccttatcag ccatcttttg aaataaaagt gcatttagta
    30601 gaaattaaaa ggaactcctc tagttaagaa aaaataataa gcatgttatt ttatggtttt
    30661 tccatcaata gaagaaaaga atgactattg ctcttgtgtg gttccgtcaa gatttaagat
    30721 tgaccgataa tcccgcgttc atcgaagcat gcttccatca tgaaattgta attccacttt
    30781 atatttatga tgataaatgt agtgttttag ggcaagctca agcctggtgg ctccatcata
    30841 gtctcattgc tctgggcaaa tcattaaatc aacagggttt aagcctcgtt cttcgcaaag
    30901 gatctccgca agagatcatt ttggagttag tcgcacagta tggtgtggaa tcagtctatt
    30961 ggaatcgctg ctatgaacca caggtcattg cacgtgataa aaaaattaag cttgctttgg
    31021 gcgaaagaga aattaaagtg cagagtttca atggaaattt acttcatgag ccgtggacaa
    31081 ttaaaaataa aaatggggat ttttttaagg tttttactcc ttactggaaa caatgtcgac
    31141 aaattcttaa tatcccctcg ccttttatgc tcactaaacg cccaagggga atcgaaatta
    31201 caagtgatga ccttggcaaa tggaagttat tgccagcaat taattgggca gcacgatttt
    31261 ctgaatattg gactcctgga gaagagggcg cacaaaaaaa actggatgag tttattgttc
    31321 ataaccttag cggatataaa agggagcgcg attttccagc aaaaaatgcg acctctcgat
    31381 tgtcacctca tttgcacttt ggggaaatca gtccttgggt gattttaaga gcactggagt
    31441 tagctaaact ggaacaaacc tgtgatctgg cctccgtaga acatttttta tcagagctgg
    31501 ggtggagaga gttttctgtc tatctgctct accacttccc caagctggat tgcgaaaatt
    31561 ttagaaagga gtttgatgca ttcccctggc aaaatgatga gcaattatta acttgctggc
    31621 aaaagggaat gactgggtat cctatcattg atgctggaat gagagaatta tgggccacag
    31681 gatacatgca caaccgcgtt cgcatgattg tcgcatcatt tcttaccaaa gatttactga
    31741 ttgattggcg ttcgggcgca cagtggtttt tagacaccct agtcgatgcg gatctggcga
    31801 ataacagcgc gagctggcaa tgggtagcag gatgcggtgc ggatgcagca ccttatttcc
    31861 gtattttcaa tcctgtttta caaagccaaa aatttgaccc ggatggcatt tacatacgcc
    31921 aatggattcc cgaacttgcc caattaaata gtcagtcaat tcatcagccc tggacagatg
    31981 ctgacgccaa ttccattttt ttaggaaaaa aatatcccaa acccattatt gatcaccaag
    32041 aagcacgaac taaggcattg agttactatc agcaaataaa aaaaggaatg gattgacaag
    32101 agattaacta aaatttggag agaccccatt gaataaaaaa agttggacgg agctgattgc
    32161 ctggatcatt acctttgaag caattggttt ttttttagga atgctgacgc aagcgaatat
    32221 tccctcttgg tatgaggagc tcaataaatc cggtttaact cctcctggac cagtattttc
    32281 aattgtttgg tcgattttat atgcccttct tgcaatagtt ggttgggttt tatggtgctc
    32341 tcgaaatgag tctgagatga gatctgtgtt ttatttatat tcagtacaga tgctgatgaa
    32401 ttgggcatgg accccgcttt tttttgtgct gcattggact gggcttggtt ttttatggat
    32461 tcttgtgatg gcgggtctca ctggtcttct gattttgagc ctaaaaaata agaaagaaag
    32521 catcgctata ttgttactcc cctatctcgt ttggcttctt tttgccgcct atctcaatgg
    32581 catgatatgg cttctcaatt aactgttccc aagttttctc ttaagccaaa tacaaactgg
    32641 ctatagagaa atcgtgataa atcttacttt gcgagatatg tacaggactt acgaaaaacc
    32701 ccaatctcta tgttaaaaaa tgtttttaat tcggtcattt agtttatcta aactccctct
    32761 ttataaacat tttttgcctt gaccttggag tttttcgtaa gtcctgaagc aatcatcttt
    32821 gagccaacag aatattgccc tctgaagaat tagggttgag ggcagttgga aatccaatcc
    32881 atagtattat cttccgcgac attgtaaata tccgaattat taacaataag agaatcttgt
    32941 aacgcagaag tgagttggca atgttttgcg ccgtgcttga ctaaaaataa aagaggggac
    33001 ttcctttgct gatctttttt taagattttg gctagggctg tctcgccaaa atcatcaaca
    33061 gcatcaacag agccgacatg ttgctggcaa aatttaatta gcaaatcact catcaaggga
    33121 tattcctcag atttgttcgc aatggtcttg attgccttgg gaaagaggtt cttttgccat
    33181 ggtgagtaca aggccgacat taatttttgc gcaacacgat ctgctcctga atccaattca
    33241 gcaattagca tgctgatttg ctcatcataa ctaccagtag cttgggataa aaaatctaaa
    33301 aagagctgtt cataatcttt ttcggcataa aattctgctg tcatgggagt atctgcttta
    33361 tcactcgcca aaaagcgctt aacaggttta tctaggttta agaaaattcg atttttgata
    33421 attgctgcat ttttcatatc acaaattact ctacctggat atataatttt cattataatg
    33481 aaatttaagg gatatttaaa ctatttccct ttttccttta caactttaaa aactggctag
    33541 tgttattaca attttcactc acctggtgtt ctctttttag aattcgatgc atattaaagc
    33601 actctattcg aaaatcatct gaaaaaattt cagcctttgt actgattact aatggaggat
    33661 ccaatttcat tttctcattg atatgagata tgatttgcgg gttatctttt aaagcatcaa
    33721 ggaaaaaacg gatataagaa cgagtttcaa atttatgcat cgctgttggc cgagcaatat
    33781 tttcccctga aaaatatttt tgcaataatc gcataatacc gctaattgaa gattgactcg
    33841 cgcaagcctc cttcaattga ttaagagcat cgatgtctgc agtagtcgta cctaaaaacc
    33901 tgctgtccag atgcctatta agattatgaa aattaatgca gtcctcaatt gatttaagca
    33961 caatatcgca taatattttt acttcaggag cgggggcagg acaatttgca agctgttcag
    34021 cctcgcagaa atctctcata cataagggca tatagcacga tttaaaataa tttagatagt
    34081 acgcccatac ttgatctctt aactgaataa aatcagagtc aaattgtgca atttgcttaa
    34141 agtattcatg ataaagagga ggatgagtta aatattcaag tccctcgtag cttggataat
    34201 taggcagaag attaatgcac ttcaccaggg gaataagaat ttcgttgaca ctgtgaaaac
    34261 cggcaccgac gatatatgct ataattccaa ggctgtaggc ttgtattaat tcgatatttt
    34321 gaggatttaa aagcagcagc atcatgttca tatagataga agtcatgcca gaagggccag
    34381 tgacataggg gcaatcattt gtaagtgcag tcttgtaata tattgaattc tgattggctt
    34441 cttgtgcttg ggcatccgca caccaagttt gttcaggaat aacggttaac cccctgctat
    34501 gcgctgaatg atccagcttc atcaatccta actggttggt ttttaagtag gttttacgag
    34561 gtccttgacg ccctctgttt tctccttggg tataaagctc atcttggtaa aataattttt
    34621 tagtgatgaa agttctaaat tcttctgctt catttttagc tgctataagc tgataggaaa
    34681 agccttcctc ttcttctttc aagtcggggt gatcctgtaa atatttatcc agaacttggg
    34741 caaaaaggcc agttggctta aatggtggca aagttcgtaa tggaaatggt ccgattacac
    34801 tgcgagcaaa cataaaggat atgaattgaa tttgaataaa attcttcgga ttattttcct
    34861 ttaaaatagc aataacctct tccagagacg gccttttttt aaaagcccca aactctatat
    34921 cagaaccctc taactcttgc aaaaaaaaat tcagcgcagc tcgttcagag gatttcgagt
    34981 ctaatttact atactcttcg attaaattga ttatagcttg agataccaga tcaattgctt
    35041 gcatcatgct tttcgttggc gattctatca agcggcaagc cagctctgat tcaaatctcc
    35101 gaggcaaaat attgtttgca ttagattgac tgtctttgat aggcaccaag cataacgcct
    35161 tcagctcatc ggcatgctcc tgctcaagca agtgactcaa gccatgtaac gttttgtgaa
    35221 cgaccaaagt atcttgctca tgattttcat aagccatttt tattgaatca atcgctttag
    35281 ctatcgcttc tcttttgtcg ccaatgtaat tggtctgcca ttgatttaat aaaaaaataa
    35341 aataagtgta agcctctggt tttgaattaa aacatcggta gtcacccgtg ttttttaatg
    35401 atgtcggatc agccaaggca gcacggttat cgctgccttg gcgttttgta ttttttagag
    35461 aaaggttaat tttttcttta cttaacatag aacgccttcc ttgaggttaa tatggcaaaa
    35521 tgatgacgtt gcggttttct ttcctgggtt catgatttgc aaccatctgc tcttcatttg
    35581 ctgcgatgga cgtcttctta atctccagga agagctcttt ttgcttagag cttcccaatt
    35641 cgcactgatg cacaccaatc gtattttcta ttcttaaggt caaattgaat tgtgaaagca
    35701 gtgttgggct ttccttcaat acatcaaaga aatgagcaat aaacttaagt tgcatttctc
    35761 tatttattat tattccttct tctgtctcaa aagttccttt aaagtacgcc tggatctgcc
    35821 taaaaattgc ggttagccct acctgttgtt gacagacatg acttaagacc tcaagcgcct
    35881 ttgtttgccc acatggtgtt gaaagaaaca accccatatc gccagtacgc ttaactttat
    35941 gctcttctaa gcaatttttc acagacttag acacaatttc aagcatttct tttacttctg
    36001 agcttatatc ctccattgag gggacttgat gtggcaagca atgactacgc atacaaacgg
    36061 gcatataagt catacggaaa tatgcaaggt actcctgcca aattttttca tgaacctcag
    36121 caaactcttt atcaaactcc tctatcgcct taaagtaatg gttatataaa ggcgaggctg
    36181 taagatattc tattccttta tgctgtggat aattaggcac aatacctacg cacttgacca
    36241 tcggaacaag aatttcttta atgctgtggt agccagcacc aactacataa gtcattaccc
    36301 caaggctgta agagagaatg acatcttgat ccttaggatt gagcagaaga aacatcatat
    36361 tcataaataa tgaagtcatc ccggaaggcc ccgtaatata agggcagtcc gttttcaggg
    36421 ctgagagata atgaattgaa tcgtagtttg gtagctgggt cttacaatca gcataccaag
    36481 attgaccatt taacgtcagt agtcctttgg tgtgggctga atgttcgaac ttcattaaac
    36541 caacttgatt agtttgtaat ctttggttag gcttgccttc gcggccacga ttatccccgg
    36601 tagtaaaaat ttcatctcca taaaaaagct catcacaaat gaatgctcta aagcggtacc
    36661 cctccccttt ttttactttc gatgggttgt gatctctaac acctgccatg tgatgataga
    36721 gctcaaaaag tgatgtctct tcaggcgcat actttgcttg atatttttgc aatatttcta
    36781 aaaacgcgcc ttttggctca tgttttggca agtcatgcaa ggcaagatcg cgtaaagcaa
    36841 cttgggcaaa tttaaaagaa atatgctgaa tttgtacgaa atcttccggc ctgtttgcct
    36901 tgagtatttt tataatatcg gaaatctcaa ttttctcttt gggggcacca aaaagaatgt
    36961 ccgattgaac catttgttcg agaaaatctt gtaaaacctc atgctcaaaa gattcggtcg
    37021 gggaatgttc atattgttcc aggattttta cgatagcatc tgaaacttgt ttaatcgctg
    37081 ccatcatttt agctgttgga gtttttatca ggcggctcac aacgtctgat tcaaaacgac
    37141 gcggcaagat gtcgtcatgc ccgagttctt gttgctggac attagcaagc gatttaatca
    37201 aaacagagag caaggcttta tgagaattaa gcagcccgcc cgctttagat aactgctcaa
    37261 gcaatgttat aatctttacg acatcctgtt catggttttc ataagctact tttatttgtg
    37321 caatgatttc ctctaactcc ttgttcattt ccggtgtttc ataacagctt ttccattgag
    37381 tgagcagcat aatcatatag gtaaatgctt ctggtctcga tttgaaactc tcagggactc
    37441 tttgtaattg caggatgcga gaaaaataaa gtgttcgtac tgatgtttca tttgacatga
    37501 gcccagtaat acatgcctcg atttcagcgt gcaaaagcac aagatgctta tttttaattc
    37561 ctatatctac tttcgcaaat ttaacctgcc tgaatggatt aaaaagttca tccagagaat
    37621 aagccaaagc aaaaagatcg ctagcctgag agaaagcaat aaaatcctct tgtgcattaa
    37681 gttctggtgc taaatagctt gcggttcccc atgataccgg tttaggttct ttatcagaag
    37741 agctgctgcc gaaatcaact aaatgacact gcatatccgc atccagcata atatttccag
    37801 gtttaagatc gcggtgaatt aagcctaatt catggaattg tttcacctga tggcatacag
    37861 aaatcataat gccgagaact tcccaaaaat gttttagatt aatattaaga ggaagcgcgt
    37921 ttttataccg ggataaatca atccctttta taaaagttgt gagaatataa tgcacctgag
    37981 tttgttcatt aaaaaaagtg cctaccaagc gacccaattt gcttaaggtg tctgtctcat
    38041 tggtaaactc tgtcaaactc tcagcattga tcttaacgga ttttaggaaa taagtttttc
    38101 catcatccaa agcataaact ttaaattgct cacaataaga tttaccacct cctgaaatct
    38161 tgttttcagt gggaacgatg attggcttaa gagacgcctc ggtggtaata aatagaattt
    38221 gacaatctat tccaaactca tgaatcatta tttcacgtga gataggcttg ccgtcggcag
    38281 attttattag ttttctaatg cctttgttgc ctaaaacctt tatcaacaat tccaatgctt
    38341 tctcgtccat gcctggcaat gattttaatt catgaaaccg aagcaatttc ataaggtacc
    38401 tcacatattt acaaacttgg ataaaaataa cctttgcatt ataaaaaacg atcattaaga
    38461 aaaagttaag agcaacgcac aaagcaaaat aatagctccc aaacatgctc tcgcatacca
    38521 aatagctgct cccaagatgg ggctcattaa gagaatgatt gcgcaaatat caaacaccat
    38581 attcaagtat cgaaattacc gatacccttt tatagtaaga aacatttatc tcgattagcc
    38641 ataaaattaa ctttttgtta aatgataggt ttgggaaatg aagctctatt acctgaatcc
    38701 aattgaatgt aaaatacaaa accagaagga tataaatcat aattaaggaa ataaaatgaa
    38761 tggatggacg cttaaggttt ttgcgattgc cttttttatc tttattgtga cagatatgat
    38821 atggcttggg ttcatcgcca gaaatcttta ttttgaccaa taccaacagt ggctgcgtct
    38881 ttcagagggg caattaaagc caatatggtg gtctgcttta ttagtttatc tgctttttgc
    38941 cttgagtatt gttgtctttg ttataccgct ggctaaaaca tcgctacctt atgccgcatt
    39001 atatggcgcg cttcttggtg ctattattta tggaatttat gattttacct gcctcgctat
    39061 ttttaaggat tttcctgtcg gtatggcatt ccttgattgg ctatggggta tagtgctgtg
    39121 ctcctggagt tcgatagcga caatctatgt ggcaggttat atcaaataag acatcagaga
    39181 ttggcagaat ccaagtctgc caatattcgc tgtgctttat ggatagtctt cttttgttta
    39241 tcaaaatcca ttttttgcca attcatatag gcataatgaa ggcgagggtt atcttttaat
    39301 ttttcttgat tttggtgaag aaaattccaa tagagtgcat taaaaggaca tgccttttca
    39361 cctaaaagat cattgggatt ataatagcag gattggcaaa aattgctcat gcggtgaatg
    39421 tatttcccac tggcggcata cggtttactg gccattaacc ccccatctgc ataaagagcc
    39481 atacccaatg tattgggtaa ctccacccat tcatacgcat cagcatagac agcaagatac
    39541 cactcacaga cttgcttggg atctaaacct gcaagcaagg caaaattacc tgttatcatc
    39601 aaccgctgaa tatggtggga ataggcttca gtcgaagtct gacgtaccac ttccttaata
    39661 caaaacattc ccgtttcctc tccccaaaaa aaggaaggca acggcctgca tgcattaaaa
    39721 tagttcatct cgccgtaatc cggcataaaa agccaataaa tacccctcac atattctcgc
    39781 catcctaaaa tttggcgtat gaatccttct accgagttaa gaggcatttg ctttgagaaa
    39841 taggcttgct ctgccatttg acataattcc agaggcaata aaagcccagc atttagataa
    39901 gacgacagtt ttgaatgata taagctgatt tcatctttga gcatggcatc ctgatacgca
    39961 ccgaaattgc aaagaaaatg ttccataaaa taatcggctt cttctaatgc ttgctcgcgg
    40021 gtcactgcca aattaaaggg atagcactga ccaaagtgct tggcaaaatg cgctgaaacc
    40081 aagcccaaaa cctcatcagt tatcacatcc ctggcatgct ctaaacgctt ggggaatgaa
    40141 ctgatgtgat tggcattttt acgattttga gcatcataat tccatgagcc gccaaccggt
    40201 ttcccatcat tatcgaccag aagatgatat ttttggcgca tcatccggta aaaaaactcc
    40261 atccgtaact gtttttttcc cttagcccaa tttctgaact cattaatttt gcataaaaag
    40321 cgattgtctt catgtagagt aacaggaatg gtcaattggc tttttaaatc atggaatatt
    40381 ttcagaacac gccactctcc aggttcagta aggcttattg ctgacacctg ctcctctaca
    40441 atggcgcgaa gcaactcttt ggcaaaactg ccttgattgc gaggatcatc gaatgccgta
    40501 tagcggactc gataaccttt ttttttcaat tgttgcgcaa aatgacgcat tgccgaaaat
    40561 aagaaagcga tttttttcgg gtgatgaggg acataagaag tttcttctgc cacttcacat
    40621 aaaaatacaa tgtccttctg tttattaatt gctgcaagtg aggcgattgt ttcattgagt
    40681 tgatcgccta gtataaaaca cagtgtcgtc ataagaatta tagtttcatt aaattttcat
    40741 cttgggccga actcggctta acccgccatc aacagcaatc acttgccctg tgatccagct
    40801 gttgtccgga ttaagcaaaa acacaattgc gtgggcaaca tcttccggaa gaccaattcg
    40861 ccctaaagca tgcattgcct ttgatgcatt caaaaccaac tgattgctta ataacgatga
    40921 ggtcagcggg ctattgatca ttccaggggc cacgacattc actcttaaat tgtttgaagc
    40981 ataagtggct gcagcagatt gagccaaacc aataatacct gctttggcgg ccgcaatggc
    41041 ttcatgatta gccagaccta ccaaagcggc ggctgatgat atcaatacaa cagaacctcc
    41101 ttgattcata gttaagccag cagctcgaat ggtagcaaaa gaagtagtga gtgacgcatt
    41161 gatcgcggtt tgaaattcat caaaactggt gttgtgggca tttttcagaa gtagtgaacc
    41221 cgcacaattg actacgccat caaggggtcc tgtttggtga aaaacatcta aaaccgcatc
    41281 aaaatgagtg gcctccagtt gaaaatcagg gtttatctta tgattatctc gtgctgtggt
    41341 aaaaacggta tcaccacgat tttttaaaag ggttgtcacg gattgtccga tagcactgct
    41401 tgcagcaatt accaagtagc gagccatatc attcttcttg cattagtatg aaaataccta
    41461 ctatgatgga ttagcttgga aacttcaata ttcattatac ggacatccca tgccattgga
    41521 taaattaact ggtataacat ttcatgctgc aataatagcc ggctgtcata ggctgattcg
    41581 ccataaacaa atactcaacg acattaatgt ttttccggta gccgatggag acacggggga
    41641 taatttatcc tctactggta tggcaatcat tgagttttca tcagcaaaaa ccaaattaag
    41701 aggaactctt gaatccattg ctgatgcaag tattatcggt gcacgtggaa attccgggat
    41761 tattttttct caattcttta acttattagc caagcattta cccctcaaag aagaactcta
    41821 ttttaatgac ttttccaaaa tcatggttca agttggtaaa gagattgtct ctgtagtaac
    41881 taatcccgta cagggcacta tgataacgct cattcaaaag tgggccaccg tgtgtgagat
    41941 aaaacaaccc gactccttgc tagctcaagc tatgttggaa ttgctacctt tactctccgt
    42001 ggaagtagaa caaactaaaa attcgttaaa tattttgcaa aaagccaatg ttgtggatgc
    42061 tggtgcccta ggattttatt atttcgttga agggtttaca cagtttttac agcaatcaca
    42121 caccacagat acagcaaata cccactcacc gttatctatc acagagtacc cccagttgac
    42181 atgtaccacg acagaatatc gatactgtac tgaagcagta ctcaaatccg cctccattga
    42241 taaaaataaa ttattacatt ttttaaaagc acaaggagag tgcgcctctg ttacgggcaa
    42301 tgatcgaatc accagatttc atgttcatac caataatcct cgtgaaattt ttacgagttt
    42361 atataaggat tacacgattc aataccctaa agtagatgac atgcaaaggc aacatgaact
    42421 gcagtttggg aaaaaatata agatagggtt ggtcaccgat tcaagcgccg atttgcctca
    42481 ggatttaatc gatgaatatc aaatccatca aattccttta aatattcatg ttgacgagca
    42541 ccatttactg gataaacatt gttttgaggc caattatttt tatacacaac ttaactcgtt
    42601 cagaacttat cctaaaacct cttgtatgaa tccgaatctt atagaggaaa aactggcaaa
    42661 aattgcctca cactatgatc atgttcttgt tctatcaata tccagcaaaa tgagtggcac
    42721 ttatgacgcc ttccttactg caaccaaaaa atacaccaat atcaccgtat tggataccaa
    42781 aatgaattct ggggcgcatg gattgctttt acattatgca gggcaactaa ttgcctcagg
    42841 gaatgacatc gataccatag ccaaattact cggcaaagcg ataaaaaaca cttatatttt
    42901 tgttatggtg aaccaattcg aatcgatgat tcgttccggt cgggttaata aaatatccgg
    42961 gcgaatcgcc cagtgggtta atataaaacc catagtctcc attgattcag caggccaagg
    43021 cattatgata ggcaagtgtt tttcaacctc cagagcacgt aataaattaa ttaacttcct
    43081 tcaggaaaag aaagacgagg ctcaactcat tttagaggat tactgcatta ttcatgcggg
    43141 tgacaaaaac caggctttgc atctcgctga gatgacaact caagtattca ataaaccacc
    43201 cacgtacata gagcctgtat ctcttgcgat tggtttacat gccggtcaag gatgcattgc
    43261 tatcgcaggt cgcctgagag agcgtaatga ttcttaatcc tttgcatata gttcattacc
    43321 tgtcgtacta tctgtgctgg atattttgta tctacctagc ctcttatggc taccccttgt
    43381 tgggattaat cattaccgtg ttaatcatgg gcctacagat tattgtacag atacttaatc
    43441 aactaccctg gcgcgaagcc ctctattttt ccattggact agccttgtta ggtgcgatca
    43501 ctgataccat ctggctttat agcggtatga tctactttaa ggccaatttc tttggatctc
    43561 attttcctcc gctttggatg gtcgctctct ggctaagttt cggatttaat ctcgttgttt
    43621 tgtatggttc ctggttaact tactattttc tttgggggtt agtcatgttt ccctccatta
    43681 tctttgccta ttggctagga gtacaatttg gagcggcaac ccttatcggc agtgaaattg
    43741 tattttacct tactttaggg ttaacctggt ctctattctt gcctttgagt ttatacctat
    43801 ttaattacca gcatgggaaa tgaataagat gataatttta cttgtgatcg ccatcatctt
    43861 tttacatatg tgcttgattt ggctatggta ccgttacacc aataatcctt ccgtggttga
    43921 tgtgggatgg gcttctggtt taacactcgc cggacttatt tatttaaata atcaagaatt
    43981 ttcattgcgc tctttactgc ttagcctggc attgcttacc tggggaatca gacttggagg
    44041 gtatttatgg tggacacgaa tacgccttaa aaaaatagac aagcgctatc ttgctttaag
    44101 caacgattgg aagcttgcca aacctttggg atttttttta aattttcagt tgcaaggcgt
    44161 attgatttgc cttgtttcca ttccctggta tttttcttcc tcggaaactc aaacaacatt
    44221 aaatctattg gatgggctcg cattgattat atttctcacg gccttaattt tggaaacagt
    44281 tgccgacagc caattgcaag attttaagga aaactatcct ggaaaagtct gcaatcaaaa
    44341 actttggcgt tactgcaggc atccaaatta tttttatgaa tggctggtat ggtgctcctt
    44401 taccttattt gccctttccg ctccttatgg atggatagcc attatttctc cattgaccct
    44461 ttatttaatc atgacaaaaa tcacagctcc catgactgaa caaggttcga ttgaatccag
    44521 aggcaatctt tacatcgagt atcaaaaagt gacccccatg tttttcccta aatggcttaa
    44581 aaataattcg acgcccagaa aataactagt gataattttc tgatgattta ccccgaaaaa
    44641 ggtaataagc ataaagggta tatccaagaa ggattggcag catgatagcg acacctatca
    44701 agataaaaac aagggtggaa tcaggtgccg ccgcctccca aagagtcacg tgatgaggaa
    44761 tcaggtaagg gtagacactg attgcaattc ctatatagga gcataaaaat ataatgatac
    44821 tgtaaataaa cggtttgtat tcactaccgg aatttaagtt ctgccatacg aggtaaatag
    44881 ccattaaagc tatcaagggc aaggggctca gtagtagcat atttggaaaa ctataccaac
    44941 gatgaaacac ttcctcacta tgcaacggtg tccaaatact aacgaaaagc aggaaaaagc
    45001 tgaccaagac taacaagcct ctggcataat gaaccatttt acgctgcaat cttcccgtgc
    45061 ttttgataat catccaggta gaccccaaaa gcgcataccc acacaccaaa gcgataccag
    45121 tacacaggct aaaaggggta agccagtccg gatcattgat agtcatcgaa gattcattga
    45181 tagaaaaccc ctgcacaaag gcgccaagaa taaccccttg caaaaaagtg gccgcaaccg
    45241 aactgattga aaatgaccaa ttccacaaag tttttgacct cttggcctta aagcgaaact
    45301 caaaactcac accacgaaat atcaatgcaa tcagcataag catgataggc atgtaaagga
    45361 tgggcaaaag caaaccgtaa acttgtggaa atgcaccata aagcatggct cctccaaaaa
    45421 ccagccaggt ttcattgcca tcccaaacag gtgcaatcga gttcatcatt ttatcgcgct
    45481 cgctctcact tgaggtgaaa ggaaataaaa taccaattcc caaatcaaaa ccatctaaaa
    45541 taacatacat caaaacgata aacccaagaa gtgtagcgaa aataaacggt aacattattt
    45601 tttctccttg gggaaatcgg tcatatattg aaaagcatga tgttccgctt catcctcttc
    45661 aagaattcca ggtccatgag agatgagcct cagcagataa tagagataaa atccaaagac
    45721 cactccatag gcaacaatga gcaagacaaa agaaataata acttcttcaa cccctattgc
    45781 agaaacagag tctctcgtct ttaaaagacc atggacaacc cagggttgtc gcccaagttc
    45841 agcggttaac catccagcga tactggcaat aaaaccgagt ggtgccatta caatgcacca
    45901 ccgatgaaac caggaatttt caaaaagagt tcctcttaag cgcaagataa aaccagcgat
    45961 agccgtaaat aacatgatga gcccaatacc caccataata cgaaaggaaa aaaatacagg
    46021 agccatgcgc ggctgctctt cattgccaac agaactcaaa ccgatcattt caccgttcca
    46081 ctcatgtgta ttgatcaaac ttgccagttt tggtattccc acttcataaa aatttttttg
    46141 ctgtgtctgt gaagggatag caaacaacaa aagcggcgct cctttttgag tttcccatat
    46201 gccttccata gcagctgttt ttagtggttg gtaatgatgc acattaatac ctacgacatc
    46261 acccactcct atttgcaaag gaactaaaat caacgatgcc cataaactga aagataaact
    46321 ttttttagca attgcagtag attgtttcct taaaaagtaa aatccggata ccgccatgat
    46381 gacaaaacat gtcgttacat aagaagctaa cagcatatga atgaatctag gaatgaaggt
    46441 aggattaaaa acaaccgccc accaactatc gacaacatat ttcccttcaa tgagttgata
    46501 cccgctgggt gcctgcatcc atgaattggc tgacatgatc caaaaagcgg atacggttgt
    46561 tcctatggta accaaaaggg tggcgataaa atgaagtgct ggaggaactc ttttccaacc
    46621 aaaaagcatg actcctaaaa aaccagcttc caggaaaaaa gcggtcagag tttcataagc
    46681 aaacaaagcc cccaggacgt ttccaaattg agtgatgaag ggtccaaaat tggtaccaat
    46741 ttgataagcc aacacaatgc cggagacaac tcccatacca aaagtcaaag caaaaatctt
    46801 agtccagaac ttgcatattt cgagataaac gggatttttt gttttcagcc acagggcttc
    46861 catgataaca agaaaaaccg caaggccaag atttaaagtt ggaaatagaa tatgaaaacc
    46921 aatgctaaaa ccgaactgaa tgcgggataa taactctaca agcattggcc acctcaaaga
    46981 tataagttat ttgaatatat tttagcatat aagcctgtgc ccatcttaat gaattagcaa
    47041 taatattcaa aaaggaaact atggcattca cctactctta attggtatct gctgcatttg
    47101 cttcattatt ggctttatag catttaaaat aacttccatt caatttaggt aaaagctgtt
    47161 ctgagaaaaa tggttcttcg gtattccaaa ataaaatgaa agctcctaaa tactcttttg
    47221 aaaaagaata gaagtcatca aagcgataga aattgcctga cttaggattg gtataagtat
    47281 aatctggttc ttgaatagcc atagctattt ctatctttcc tttgaatttg tgaaaaaaag
    47341 gataactatt tttcatttgt gattctttat agggcaccac atcaggtcca cctaaaccga
    47401 tatggtgttt tctggcaaaa gcaaataatc ggcttatata gtgatggtca ttattccact
    47461 cgtctggaaa aaaattaaca tattgagtca ccctggtttt ttgaaacacc ttttttaaat
    47521 aactcatatt atcaagaact gaatagaaat attcgtctga gctaaaatct tcggggagat
    47581 tataaggatc aagatctatt gaggtttcag gaagattaat accataaatt tttccatcaa
    47641 attttgatgc cagttttttt aataatagct gaaagcgttt tcttacagcg gggtcccagg
    47701 tacgagctac ccatcccgcg gtaactggtt ttccttctcc ggggaaatca tactgctgta
    47761 ccacacctcc gtgatatgct ttttccaggc gaatgtagtc aggtacagga aatacatctg
    47821 ggctaaatga acggtcttgt atttgaatat acagcttttt atcgattctg ttgaggtcat
    47881 tcaaatcttc ctcaattttc gaaaaatcgt aaactccttt ttgaggctcc aattgcctcc
    47941 aggaataaat gatttgtacc ccacgaatgc aaggattttg caaagtctcc tgaaatgtac
    48001 ttgctctgtc tccccctaaa aacagaaaca attgtggaaa ctcagattgc gcgaaaccaa
    48061 ggctattaaa aaatagcaaa agaaatgaaa gaaatactct catgattgtc attttggttc
    48121 ctgcttattt catagcacga tcagtaacta attccttacc aattttcact ctatataaaa
    48181 atactctgct ttaaaacaag agcccatgat gttaccattg aaaaaatggg caccaacatg
    48241 ctgcaaacat ggtaaaagag ctcaaagtat ggagtctaaa aattgaatga atattctaat
    48301 gattgtaaaa catttccatg taatcgaata gggttaattg gtcatttttt ttcaatctga
    48361 agcaaaaatt gtcacttttg aatttttaaa gcatgataaa aatttgccat aagtgaagcg
    48421 cataagaatt gcttgaacaa acgttggact aataaagaaa gggaacctca agggttccct
    48481 gatttacgca ttaccccagt aaaatccaag cctctcgcca tgccaaacct acgccaggtt
    48541 cataataagc tggcgcttgt gaacaccaac cagaataagg ccagggctta cactggtaat
    48601 acttgcgatc aagacgaccc agaactatgg ttcctgcctt ataggtgcta ataccatctg
    48661 ggtaaatcac atcagaatta cctgaaggct ctacaaaatc aacgagaaca cggtagctgc
    48721 tgatatcgtt gtcgctaagg tatatcttat tggcctgatt ggctgccaaa acgacctgac
    48781 cactttgcgg atcaagctga ccaatacgta ccagtgagga gccgctattg actgctgtgc
    48841 ccaaagccag aggccattgt tctgctgtac ggttgctttc attggcggtg atactggtgc
    48901 tttcaacttc tacttgattc ttaaaaactc gtagagtgat cttagtgttg gcagcaatat
    48961 cgcccgaccc gtaaaagttc tgcaattcaa accaggttgg ggcctgtgct gtaccatcta
    49021 taaatacatc actacaggaa tagaatgcct caggactgtc ctcacgttgc caaataacgt
    49081 aaaaaattcg tttgcctgtc ctattcgcag gcaatgggca gtccatctga taacgaccat
    49141 tcgctaaagt gacgctggtg attgtacaaa acgggctagt ctccaaatcg gaccatttta
    49201 agggggtatt gaaatcatat ccgtccttag tgacataaaa tttaaaatac ttggttttat
    49261 gtggagcagt agcaacataa acaaactgaa agtggccatt agaatcagga tgcactaccg
    49321 tggctgtcca atcggtgcga gccagattga aacctttaaa gaaatctcta cccccggcgc
    49381 ataattgccc gtctgcaatc agttcacgat gtcggtcatt agccgctgcc tggttaactt
    49441 cgtgccaatt gtacagaggt tgggttccgc ctacctggac agccgcttta caagctgctg
    49501 atttagggct ttccgggcct tccttataac attgataggt ccgggaaata gggacttcca
    49561 tagacccatg cgcaaatagt gaaatgctga ctactgcaag accaaggctt cccaatattc
    49621 ttttcattaa tccccctatt atgaaattta agaaaggctt atatacacca gcaatgttca
    49681 atagattttt taatctaaca caaattatct ttatttaaca atgaattaga attgttaaat
    49741 aacctcagct cttttcctat tactgtccct aatctgttgg gacttttaat tctttgcaaa
    49801 aaaaccacat cccatttttt gcagttttat ctgtaaaggc aaattttaat gcactaccac
    49861 ttctagaggg gataggcata ttgctaatta aatttagaat ttaatctgcg ttaaaaagta
    49921 taaaccaact cattgtcaat ttttggggcg cgcgcactag tatttataga ttaactcaag
    49981 tggaaaataa aaatacttat ggattaaata taattcaaaa ataggttacc caaatcactg
    50041 catcattcta aattctctca aaatatctct tgcccaagaa aacaaaaaag acgacgagtg
    50101 ctaggagtag ataagtactt gcaatagggg gtaggcagag cttaaatttt gtaatcaatc
    50161 attcgccgaa ataaaaataa tctatcaaat aatcacggcc gttccgtagc ataaaacttc
    50221 tgtgccggct tgcccaattt cacctgtatc gtaacgcata gcaattatgc cattggcatt
    50281 gagtgcctgt gcatgttcta tcatgagttg gaatgcttct tctctggcat gctcacacat
    50341 ttgcgagtac gcaccaattt ttcctccgac gatcgatttc aagcctccca tcaatccctg
    50401 gctgatggta ggagagcgca ctactatacc tctcacaata cccaaatatt gtttaatcac
    50461 tttcccttca aacgaatttc ctgttgtaat catcattgtc ataatccgct cactaaatat
    50521 cccttttatc tttccatatt agtttaaaat cttgaaaata gaagtttatt gaaaaaaatt
    50581 cttaaaacta cgagggaatt ctaaataaag acccccttat ttataaatac aatgcatttg
    50641 ccggattaca gattagcatt ggcaagaata gtttatcctt tttattttta ctgagaaatt
    50701 ttccctagta ccagattagt tttttttcat ctaatgaaca ggataaaccc aaacgataac
    50761 tacaacacat cttggcaagt aaaatagcca gaatcaaaat ttcagcaagt tttcttcaga
    50821 aaacaaataa gaaattcact ttcttactgt cttaattctc caaatcgcaa ttcaatcaga
    50881 aaagttattc tccagacaac cacaccccaa catgttgtct attatatgag cattgaaatt
    50941 taatctacta tacttaaaga tatacttcac atggagcatt gtatgcctaa aaccaaatat
    51001 gcccttcctc cagttgtgtt atacgaaagt catgcggata gagctacgag tgattttctt
    51061 atcaagcaac tacctgattt gaagaaagct gggtatacaa ccatttgcgt tgatggaatg
    51121 gaaccaggcg catccctgga ggaaaatatt tctatgatga aaatactcat caagatacaa
    51181 atcaagaaat tatctgagct acctcttgaa caccctgaat atgagcaagg aattgaaaaa
    51241 ctgagaagtg ttgttgccaa attggagctc tttgaggcga tgaaggaaca aggattcaaa
    51301 cttggtggaa ttgacttacc ggtttcagag cagcttaaag agaagagcct taattccata
    51361 agaagagaac aaacgattac cgataatacc ttaaggcatg tgaaagaaaa tgatggagga
    51421 gtagtcgttg tcctgggatt tggccattgc atattccagc aaatgattaa agaacaggat
    51481 gaaaatgcca atcaatacct ctggtaccat gtccataacc cggataatga aacacaggcc
    51541 tacaaagaat tgatcaaatc ctacacaaaa aaaggacttt ctacttattt cccattggga
    51601 gttaatattt ttaaaagctc agataaagaa ttggataccg atttttggaa taaggtaagc
    51661 gccaattgct ataattatga tccaaaagct cttgaaacat cgactgcttc cattttgaag
    51721 tccttactgg gtcctgaagt ttcagcacac ttgcgcacag atggacaaca tcatgtggat
    51781 gcactgatat ctcttgaaac agttgaaaaa aaacatcagg taaaatcttc ggatttcttg
    51841 agaagtttaa gtaaaacctt aggtgacatt cactttgaag tagcaaaaat caaaaccaaa
    51901 gatcaggtga ttattcgtgg cattaatgaa cctgaggtag cagagcagat cagtaagtta
    51961 tcaaaaaaaa tgtaacccta cttttttgaa aaatatagtg tactttaaat tatcttgttg
    52021 aataaacagt taaatgtgtt attgctgctt aaaagtaaca tggagtatac tttgcctttt
    52081 ctgtatagaa aagggagtca tttctatgtt caaacgaaca atacctctgt ttgcagcatt
    52141 cactctggcc atctcgcctt ccgttttccc aaatgatgca catgcgcaag aagacaagcc
    52201 gaaaaccaat caatactggt ggcctaaaat gctcgatctt agcccgctgc ggcaacccaa
    52261 tgccacttcg aatcccatgg gtgaaaaatt caactatgcc gaagaattca atagtttaga
    52321 tttgaatgcc gtgatagaag atctcaaaaa attaatgact acctcgcaag actggtggcc
    52381 tgctgattat ggtaactatg gtccattatt catacgcatg tcatggcatg cggcaggaac
    52441 ttaccgcatc tatgatggcc gtggtggagc aaacggtggc ttccaacgtt tcgccccgca
    52501 aaacagctgg ccggataatg ccaatctgga caaagccaga cgtttactgt ggccaattaa
    52561 gcagaaatat gggcgcaaga tttcatgggc tgatttgttg gtacttgctg gaaatgttgc
    52621 gatggaatct atgggcttca agaccattgg atttgctgga gggcgtgaag acgcatggga
    52681 ggccatcaat atcaattggg gccctgaagg caaatggctg gaaagtaagc gccaggataa
    52741 agatggaaaa cttgaaaaac cgcttgctgc gaccgtaatg ggcttaatct atgtgaatcc
    52801 ggaaggacca aacggcgttc cagatcctct tgctgcagcc gaaaaaattc gagaaacttt
    52861 tgggcgtatg gccatgaatg acgaagaaac ggttgcttta attgcaggcg ggcacgcatt
    52921 cggaaaaaca catggagccg cgtccggcaa atatctgggg ccagcgccag aagcagcggg
    52981 catagaagaa caaggctttg gttggaaaaa cagttacggc agcggcaaag ggaaagatac
    53041 gattaccagt ggtttagaag gtgcctggac cgtaacacct acccactgga gccataacta
    53101 cttacaaaac ctgttcaatt tcaattgggt taaaaccaag agccccggtg gagcaatcca
    53161 gtgggtgcct gagaattcga atgcctcgtc aatggttccc gatgcatttg accccagtaa
    53221 aaggcatgcg ccggtcatgt tgaccacaga cttggcacta aaatttgatc ccgtttacag
    53281 taaaattgcc aagcgttttc ttgataatcc taaagagttc gatgacgcat ttgctcgtgc
    53341 ctggtttaag ctgattcacc gagacatggg gccacgttca cgttacctcg gctctttagt
    53401 gcctaaagaa atcatgattt ggcaagatcc agttccacca gttgattaca aattagttga
    53461 tgccaatgat attgctaacc tcaagggtaa aattctcaac tcgggattga ccacatccga
    53521 gctggtcaaa actgcttggg cctcggcatc caccttccgt ggcactgata tgcgtggagg
    53581 ggcaaatggt gctcgaattc gcctggctcc ccagaaagat tggccggcta atgatcctca
    53641 ggagctggca aaagtattaa aaacattgga gagcattcag aataacttca ataatgctca
    53701 agccgatggg aaaaagatat ctcttgcgga tttgatagtt ttaggtggta atgccgcaat
    53761 cgaacaagcg gccaagcaag ctggctacga catcattgtt cccttctcgc caggacgcac
    53821 agatgccaca caagaaatga ctgatgtgaa gtcttttgag gttcttgaac ctacagcaga
    53881 cgggtttaga aattactttg ataaaagcaa caatatgtcg ccaccagaaa tgttggtgga
    53941 aaaagcgagt ctgttgaaat taagtgttcc ggaaatgacg gttttagttg gtgggatgcg
    54001 tgtacttaac gcgaatactg gacaaaatca atatggtgta ttcactgaca aacctggaac
    54061 tctaaataat gactttttca tcaatctgct aaacatgtca acagaatgga agaaatcaag
    54121 tgagacagag gggatttatg aaggttatga tcgcaaaact gggaagctga aatggaaagc
    54181 gacctcggtt gatctcatct tcggcgcgaa ttctgagtta agagcagttg cagaagccta
    54241 tgcaaccgat gacgcaaaag aaaaatttat acaagatttt gttaatgcct gggttaaggt
    54301 aatgacagcc gatcgcttcg acatcaaagc agcaaatgcc aacattaaca gttaatcctc
    54361 attctttcag atttagggtg acaaactgtg actcaatcat cgtttgttac cctaagtccc
    54421 aaatcaccgg ataagactct gacttatact ggtttaacaa agcccagcta atttagccaa
    54481 acctctatgg ttttccagat tggcctaggc tctctcttga attacgtgtt atacgacttt
    54541 actccatttc aaatattcgt cactttgtac ttgaaaaaat ctggtttaac ccaatatctt
    54601 gcatgtggct ttcatgttca ccaatgagag ataaccgctt taaaataggc ctcttgccta
    54661 agctgattaa ttggtttcag gccaaggcgc taacgttttg aggagaggag tttgcatgca
    54721 gcaaatgagc actgcagaaa cgtttgcaac gcagcaataa aatgaaagag cgcaggttat
    54781 gcaagagggc taataagcgg attgaagtaa aaacatccat atcaaatgga gatagaattt
    54841 tttataaaca aatcttgtga ggagattttt aatgaaaaat aaactacttc ctaatttaat
    54901 catcattgct cttgccgcta cgatatctgc gaactccata accgttcagg cgaaacaaaa
    54961 tgatcaaata caaaatagta acgaacaatt aacgcaggat ccatttgata ctgacccttt
    55021 ttttcaatca catgatgatg ttctgctgca aatacacaaa atgcaacaag ctatggatca
    55081 attgatcaaa agtcaatttt cacaaatgca aaatagcctt gcaaacgatc ctcatcacaa
    55141 tccctttggc agtgcaaaaa atattcaaat cacagagaat aaagatgagc ttatttataa
    55201 gataaaacta ccgcaagatt ctgatagtaa agtggatatt accgttaaaa atgggcagtt
    55261 agttcttagc gccaacgtca ctcagaaaat cacccgtgaa caagataata acaaaagtgt
    55321 cagtttctcc cgaagtaatt acatgcaatc atttccattg ccccagagct atgatccgaa
    55381 gt
//
LOCUS       DASLOE010000005        45607 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.005, whole genome shotgun sequence.
ACCESSION   DASLOE010000005 DASLOE010000000
VERSION     DASLOE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 45607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45607
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.005"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            151..1203
                     /locus_tag="U6309_000210"
     CDS             151..1203
                     /locus_tag="U6309_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503681.1"
                     /translation="MDYVDDFISIEEHSSGDDVTNELSLAEQVFTRLQIYSEGFKPSF
                     FIHKDLANLSLTSKTLNGVVCSELNQRAAQKLLTHVVKGEQDKAQAMLEKNPGLLLIK
                     SKAVDYSGRTIVGTAFQGAIGAGDKPMWKMMLSYFEGLEQGEALRQFDEQFPSGTEET
                     PAAELKAYYDAIASAIIHDEDHGLSVIEGFRKEITCQKEITQGKHFNLQHLIAAYQAY
                     IDNFYAFGNWGNRDLFWQKVIGYVQRQMTAYDAQIYCSGVISVLYNESSFSRTFELSD
                     DKNFFPLQIDSALGFDFACYSYYSARALDSGSHHVLPSPLNEYWIWIEKLCGEKTDAL
                     VGLRESLEQGVYYQLK"
     gene            1484..1594
                     /locus_tag="U6309_000211"
     CDS             1484..1594
                     /locus_tag="U6309_000211"
                     /inference="COORDINATES: protein motif:HMM:NF017416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN5503682.1"
                     /translation="MFLLQQLFNKTDPQIEYDVKDNAAYQLFCGIGIVEQ"
     gene            complement(1793..3073)
                     /locus_tag="U6309_000212"
     CDS             complement(1793..3073)
                     /locus_tag="U6309_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEN5503683.1"
                     /translation="MELTTTENQLFKSSKVSLALVTVLMGIGSGFLGMCLAMLLHYLQ
                     HIAYGYSPLHIISNETFLEGVRASSSERRISILFLCGLIAGMGWWALYRFGKPLVSIA
                     TAIKSGKPMPVLSTFIHAFLQIITIALGSPLGREVAPREVSSVFAYWLSSKAGLTPEE
                     SKIMLACGAGAGLAAVYNVPLGGAVFVLEVLLCTSNWTVLLPALSSSAIAVLVSWWGL
                     GNEAIYQLPEFSLSAPLIIWSILASPVLGFFAFWFIQIATIQRSKAMHNWQMILACLI
                     NFLIIGLFAIYFPSLLGNGKSPAEMEFDQSVGIGLSIILFMLRNVFVWSSLRVGAQGG
                     LLTPSLANGALLGAILGGIWNLYLPPAPIHAFALIGATAFLAAAQKMPLTAIILIFEF
                     TRINFIFLIPIMLAVSGSIAMCQLTKNYYIKYKV"
     gene            3550..4830
                     /locus_tag="U6309_000213"
     CDS             3550..4830
                     /locus_tag="U6309_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5503684.1"
                     /translation="MNTLSFKHPLLAKTYLWIIWTIAACFFFYKYLIQVSPSVMTADL
                     MKAFQVNAAGLGNLSAFYFYSYLFMQIPVGLMLDRYSPRLLTTLAIFVCSISTYIFSQ
                     TNTLWLACLSRALMGAGAAFAAVSCFKLAAVWFSPKRFALVSGMFMTAAMLGAVGGQM
                     PLAFLVQHEGWRKALELVSLMGIVLGVVYFLILRDKPAQTQPTQNSKEPVVQLLRRII
                     VNKQAWALSLYSGLAFAPVSVFGGLWGVPFLEKAYLLSRTDAALAISFIFVGFAAGAP
                     FWGWFSDFIRRRKPVLFTGTCSALLCLLVVIYSSNQNLLTLIILLFLFGFGASGFFTS
                     FAMIRELFPLVLVATVLGIMNTFNSVFEALFEPLVGALLDWTWEGTVVDGIHQFTLHG
                     YYFSLLLLPLSLILALLTLLLIDETYCRTKEEVE"
     gene            complement(4840..5259)
                     /locus_tag="U6309_000214"
     CDS             complement(4840..5259)
                     /locus_tag="U6309_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503685.1"
                     /translation="MCNWNRIIGLALMGFSTFLQAKTYIPQCPKEINTLERIQTSPDG
                     WETLSGIKNNYLGNVSFYSGHPKGQASLKPNRINKKKAEWKFSPHDTIYIVCHYNQTG
                     IELTQQLPQKTKGCTVIFNPNAKGPYGFLPQKITCNQ"
     gene            complement(5313..5579)
                     /locus_tag="U6309_000215"
     CDS             complement(5313..5579)
                     /locus_tag="U6309_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503686.1"
                     /translation="MESMIDLVGYVASSLVFATFYVKKILTLRLIAVCSNVAFITYGL
                     GYHLHPIFILHSVLLPLNLYRIFELLKNDPEFTILARNNLKATR"
     gene            5691..7412
                     /gene="legLC8"
                     /locus_tag="U6309_000216"
     CDS             5691..7412
                     /gene="legLC8"
                     /locus_tag="U6309_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegLC8"
                     /protein_id="HEN5503687.1"
                     /translation="MIFELNILPSRNFLFIFGDNMPLTSLKKFLKDIGKENIKQHQVK
                     LVLKTSQDIQLFLNALRDSRNHGISSLSELDLSDTRFTNQELSDLVTALNNIPGIKSL
                     RLDSCGLKDSDTVELSKLTHIKKLSLKSNYLKNRPMFNSMLEALYLDYNTELSASYVL
                     FSLSRNAAALKKLSLRNCGVTDANLEYLTRPESRLKSLTHLNLRRNNITHQGVDSIAH
                     LQSLTTIDLSQNTGIGDEGVSRLALLKQLRTLYLDNCGIGAEGIKAIAKMNLQTVDLS
                     FNPGLKKEWGLDDIRPNHTIRTLLLTFCSLNDNHAKLIVSKFPAATDLNVANNNMTRA
                     GVKTLLSNPIIENLDVSTQSLYAKSTGASGTGMISPRIMMKKKEIKQQEKEKAQDLLD
                     TICNTITLKSINLEHTGITSRMLLSLIPDETDHKRYLKKINGVSCKELKPKLEQQIAL
                     RKAEKQSSPPVIIAVEDSKPSDLMIKSGSSSKTEAQTREDGLIASPDEKIKALELENQ
                     KLKDELATARKRIFELENQSLSGSKTRKKTSIPPLAIPVMFQDFAKPGKNETGDNTSN
                     SITAIPK"
     gene            complement(7476..8441)
                     /locus_tag="U6309_000217"
     CDS             complement(7476..8441)
                     /locus_tag="U6309_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN5503688.1"
                     /translation="MDLSKRIEPKTWSFVEKIQNAGGKPIYDIPVAEGRAIFDQLQAL
                     PNEKPDVDVEDKTLPVGPGGKVDIRIVRPKGAKEALPVIMFFHGAGWVFGDYQTHGRL
                     VREIAVGTHAAVVFVKYTLAPEAQYPTQIEESYAAMKYIAEHGKQFNLDTSRFVAAGD
                     SVGGNMTSVMTLLAKERGGPKIDYQVLIYPVTDANFDNASYKEFAEGPWLTKKAMEWF
                     WDKYLPNKEKRKEITASPLQASLEQLKGLPPALVITGECDVLRDEGEAYAHKLNAAGV
                     TVTAVRHLGTIHDFLMLNDLSETPACRNAVETIIGHLTHIFGKKK"
     gene            8670..10004
                     /locus_tag="U6309_000218"
     CDS             8670..10004
                     /locus_tag="U6309_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg1888 family Dot/Icm type IV secretion system
                     effector"
                     /protein_id="HEN5503689.1"
                     /translation="MLQFFEKGLQYSGENISADSYLMRKIIEAAIPSADKGTVSAEKM
                     LGMVNPNQIEIVSGSENAYRLIVHKIKEAKKQILIQAFVWEPGTQVVKDIRNALCDIK
                     HEVEVFLLVDQLDRWAQVFYHGEILPKKPKHDPACLGLDNLPQNIKLHIGTHVHNSIA
                     SNHNKAIWIDGDVILTGANFQQENYGPNGFHDAAMFIPGGAAEAVFYDFKAMWDKRTN
                     KSDAPEISPIYTGQESSTEFYRNTNSCSVLYVTNTIRQKWALLPFYRAPLPEDPLNNA
                     YMAAIKYAKHTIRIAVPNLNSPEIIHALADFINNRNGHVYLLMGKGFNDFRERFYGGT
                     NQSSIDYFSSLLDEDRQNNLQVRWFHRVNDNEISMKKEVIHMKFMSIDGQVVIYGSSN
                     LDLLSLHNSHETNIVIDNADFAQRAIEQLYLPIFETSIPVLISPKRTCFVCS"
     gene            complement(10186..10539)
                     /locus_tag="U6309_000219"
     CDS             complement(10186..10539)
                     /locus_tag="U6309_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503690.1"
                     /translation="MKKVIFYALPSLMLAILFSPAYADETPAQLTGTPAEQIQQLNAQ
                     IQSQLQEMQTKQQQQLDALNTQLQNQIKQVQSQLQEQIQTVNSQTQDQIKQVQATLQE
                     QIKQVQQQALQKANQ"
     gene            10696..10971
                     /locus_tag="U6309_000220"
     CDS             10696..10971
                     /locus_tag="U6309_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEN5503691.1"
                     /translation="MTRELCMRCYISGRVQGVWFRASAKKLAEQLMISGWARNLADGR
                     VEVFACGKEEKLEEFYNWLQKGPLNARVDACTRENLPWEDYITFDVL"
     gene            11053..12162
                     /locus_tag="U6309_000221"
     CDS             11053..12162
                     /locus_tag="U6309_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503692.1"
                     /translation="MELKRQCINGVVGVSLSLLAVLIHPLFAEDAPQFVTGQSQLSAA
                     RDPADSSWLPDIIGLPNEEQVESLTRHVAEVMTEEEDKNCTAIDYNLRMLVFQNFVIY
                     QHMANAQHHYFKEKMALKYAHVLAMILKESSGDPTNVTDMNGRSISTSKPGTNLQRWS
                     NLLKLTMQNGIKFNYQTNFGLTQLSADRLFVAFKLANGASHSKDFLEGKNGDETPNRV
                     DMDSAIAIRRLIWFYQDIAQGRLKQKDECINEEDIYKPEFNERYQAGLRMALLYCGTK
                     FLFRVDNESVWVNENSTFEKAMASIAYCKLGNSKTGYGTDEMDERCFAEWVTLCPALN
                     VNIALLTPLSYFQTRKNKPVCTNTLNRLLNKEPKN"
     gene            complement(12328..12642)
                     /locus_tag="U6309_000222"
     CDS             complement(12328..12642)
                     /locus_tag="U6309_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503693.1"
                     /translation="MSTPKWNLSISRAVICVIALFASMICARAHFVGGIHTNAGYVGW
                     YHPDYYNHDVIGVGNYHPGYGWVAPVYATPTYIISNTGYNCQTVQQCDSEGNCIQSQN
                     CD"
     gene            complement(12965..14182)
                     /gene="legC2"
                     /locus_tag="U6309_000223"
     CDS             complement(12965..14182)
                     /gene="legC2"
                     /locus_tag="U6309_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC2/YlfB"
                     /protein_id="HEN5503694.1"
                     /translation="MTDTPKANLCVDEIKEDTPKDQPLPEENTEILVKKLKVLETTQE
                     NFDQIKKHIAAVIDSLAKNRSFFGRVAAYWGEMQLWLKITLGIVLVVPILTLGIAIQV
                     ASLIVVSVLTLVTYVGSALLLENHVQKEEHITDRLKEEMIGLADNLGKVIESFEPLRK
                     QIAEQIEYFHKENDRLNLNVTQLSDHIMRLTKQAEQLQKTEQTLRKVREELEKTSETL
                     DEKVQVQTELLQINQEELNRIREEIAKNEIELSEKISELDQVKKEMSVEIEKAETVTQ
                     VLKNTVEKLSQTAIADHNHRESFQNKLNDFLSNKEQSFNDVAQRICEAERELCLVKEE
                     LNFSNKRYQELLERQEEVVNRLEQLQLSRKPTPYSNAKVLGKLGIMATEEKESPTPVS
                     SEENTIPTQTLTG"
     gene            complement(14420..14947)
                     /locus_tag="U6309_000224"
     CDS             complement(14420..14947)
                     /locus_tag="U6309_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HEN5503695.1"
                     /translation="MNRIKLGISNLIFMGRWLQLPLYLGLILILAGYVYRFIIELFEL
                     IFHLKGLDDTHIMLGVLDLIDVVMIANLLIMVVMGGYETFVSHLQLDSHPDQPEWLDH
                     FDAGAMKIKLALSLIGISSIHLLRTFIDPAKLSNYSIMWQVVIHLTLIVSALAIALTN
                     RLMDQPARKNLQHPD"
     gene            15182..15622
                     /locus_tag="U6309_000225"
     CDS             15182..15622
                     /locus_tag="U6309_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN5503696.1"
                     /translation="MKYLHTMVRVSNLEQSLDFYCNKLGLIEVKRTENAKGRFTLVFL
                     AAPADIEQVQTSYGPMLELTYNWDPEDYTEGRNFGHLAYHVDDIYKICQHLQECGVVI
                     NRPPRDGHMAFIRSPDNISIELLQKGEPLPKQEPWLSAPNIGHW"
     gene            15714..16778
                     /locus_tag="U6309_000226"
     CDS             15714..16778
                     /locus_tag="U6309_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503697.1"
                     /translation="MQILKSLSEGMGTAGGVAWTTFGLLFGSFGLTVGNSALTISLGA
                     ISASLFLLVAIPVTYWSYCNAHKENDELRHAIEEQENQIVHYLTEYLLATRQECLLKN
                     PQKYRNHYDKSVLAEDLKNRLLKTRLKRPVSFTTAQFLDCLLDDNHKNSILGMLIDAC
                     TMDKLVLKKKLLTIYRDSVFLQFEELSLFAKLKTGFIAFCAGFGSLAGCSSGSLGLFA
                     ALGIFPCFAAVPLVGLAIMGIACVFGLLLSGMSIDTAIQRSKEKQLFLQSEAIHHDLK
                     NLATLKTVSVKAELKAQTKYQIGAKEGLTEKLARPKLLKRSSSFDSYFYRNHPLWNIS
                     HNPPEASKCSFENLGSPFSL"
     gene            17106..17181
                     /locus_tag="U6309_000227"
     tRNA            17106..17181
                     /locus_tag="U6309_000227"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17139..17141,aa:Ala,seq:ggc)
     gene            17220..17295
                     /locus_tag="U6309_000228"
     tRNA            17220..17295
                     /locus_tag="U6309_000228"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17254..17256,aa:Glu,seq:ttc)
     gene            17352..17427
                     /locus_tag="U6309_000229"
     tRNA            17352..17427
                     /locus_tag="U6309_000229"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17386..17388,aa:Glu,seq:ttc)
     gene            complement(17468..18793)
                     /locus_tag="U6309_000230"
     CDS             complement(17468..18793)
                     /locus_tag="U6309_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HEN5503698.1"
                     /translation="MWTHRRSGQTNQRGSQDHRDPYERDRTRVIHCPAFRRLQRKTQI
                     LGTDEGDFHRTRLTHSLEVDSIGRSIVRNLMTNQEHQLLLSSLLPNDDLISVICLLHD
                     IGHPPFGHGGEVALNYMMRHYGGFEGNGQTLRLLTKVENSYGGFGLDLTRRALLGILK
                     YPVKRANVVAPKQPPIHESIHKTIKINDWLPPKAYFDCEQPEVDWLLSPLSDNDRELF
                     QSLSVKPNGTQAGKAAYHNFDCSIMDTADDIAYGVHDLEDAIHLRLINRSHLDTPEFR
                     QLISETTLSLHQERLLNSLFSPEICLRKQAIGEMVNYFITSTQIIVTNEEFENSLLKH
                     NIALIPEAHALLSYLMQCIYNNVIDSQEARTFEYGGQTVVLRLFDAISSNPASLLDNK
                     NRVLFSQAPDEVAAYRVVCDYLANMTDEYAYRMHERLFGFNTRTIFERL"
     gene            19067..19600
                     /locus_tag="U6309_000231"
     CDS             19067..19600
                     /locus_tag="U6309_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEN5503699.1"
                     /translation="MMGISIRYIILGIGVMNISSCMVYEDTYTTRYQIYTYDYGQLYP
                     QADYHARDYNYGNQSGQSVVVPDSYHVGEYHSPVSFKDRDRNWVNSQNPQGYTIQIAD
                     DEKASYVAQKLYKAPKNDRMAQVKYYQNGKAHYKGLYGTYDSQEAAQKALESLPPEIK
                     QGAGIKDWGSVQGNLNE"
     gene            complement(19703..20173)
                     /gene="lspM"
                     /locus_tag="U6309_000232"
                     /gene_synonym="gspM"
     CDS             complement(19703..20173)
                     /gene="lspM"
                     /locus_tag="U6309_000232"
                     /gene_synonym="gspM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     LspM"
                     /protein_id="HEN5503700.1"
                     /translation="MKTYLNTLNDREKWMVIVAGVSLFIYGYYLLLYAPLSNQVNQKS
                     TQLIEKTETLEWMKQVRMQKRSTKRKESVDNSQLLTILASQLKNNKTLKFPYQLQQTS
                     SGDVQLTFDAVPFQNFIQWLAKINEVYSINIKQFDVEKTSTPGVTRLMIILSTS"
     gene            complement(20170..21309)
                     /gene="lspL"
                     /locus_tag="U6309_000233"
                     /gene_synonym="gspL"
     CDS             complement(20170..21309)
                     /gene="lspL"
                     /locus_tag="U6309_000233"
                     /gene_synonym="gspL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspL family type II secretion system protein
                     LspL"
                     /protein_id="HEN5503701.1"
                     /translation="MDKCFIFSKHLDDNGCVCLKISDLGELIAPPEFRNFSQIKDLQK
                     ESSTIIVETCTRAILLGLAIPWLPERKARIAIPYALEDKVTQPVEELHFAFDKSYYQN
                     NQYLVVVIDKQRMRKLIQVFDEHGIEFDAVTLDWFALASQELIISESELLINNEDFKG
                     VLSGDLAQTYLKNHPHTPAHLFQDSKIPVASPASQNEEHSYTWISKRLIKSKPLNLCQ
                     GEIQHGKTTDWVKKGYLLVGGLCGFWLISILFVNWLSLHWLNRQIDEIDRQTAVIYRE
                     FFPDAKQVISPKFRISQLLGGSTAENQTRFWFILNQLSKAIKDSGITVEQLRYQNKTI
                     SITLVSSDFESLQKIENKLKQSQLKVKQTQASTKDQQVVATLELT"
     gene            complement(21384..22400)
                     /gene="mltB"
                     /locus_tag="U6309_000234"
     CDS             complement(21384..22400)
                     /gene="mltB"
                     /locus_tag="U6309_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEN5503702.1"
                     /translation="MRQIIWLGFISLFLLSFTTYSDTTFTKRKDVQSFINMMVTNYHF
                     NRNQLTTVMNQVKLQPQVIESMEKPYEKKNWDVYRELFLTQKRLKSGLQFWAENQKTL
                     EKIQRRYGVSPEIIIAILGVETMYGERQGEYRVLDTLATLAFNYPKRSAFFTKELKEF
                     FLLCREQHVSPTQYKGSYAGAIGKPQFMPSSYRYYAVDFNNNGRRDLVNDNGDSIASI
                     ANYLHKHGWNLNQGIAQPAKISGWRYKQLRMNPKTANYRYSQLISYGVKPITASQNHP
                     PRAGVIELVTRKGKEYWIAYPNFFVITRYNSSPQYALAVYLLSQQLKQHWYTSATKRH
                     RAYV"
     gene            22595..24427
                     /gene="lepA"
                     /locus_tag="U6309_000235"
     CDS             22595..24427
                     /gene="lepA"
                     /locus_tag="U6309_000235"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEN5503703.1"
                     /translation="MLFARRLEQLKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLT
                     EREMSSQVLDSMDIERERGITIKAQCVSLNYTAKDGKTYLLNFIDTPGHVDFSYEVSR
                     SLAACEGAILVVDAAQGVEAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIE
                     DIIGLDAQDAIRVSAKSGLGVNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGV
                     VSLVRIVNGAIRKGDKMRVMSTGRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKE
                     IQGAPVGDTLTLDRNPADKVLPGFQRVKPQVYAGLFPVSSDDFEAFREALAKLSLNDA
                     SLFYEPESSEALGFGFRCGFLGMLHMEIIQERLEREYNLDLISTAPTVVYQIVTQKGE
                     TLLIDNPSHLPPTPQIKEMYEPIVRANILVPQDYLGPIITLCVERRGVQVSMTYSGRH
                     VSVVYDIPMSEVVSDFFDRLKSVSRGYASLDYNFQRFQIADLVKMDILINSERVDALA
                     VIVHRDSAHSRGKLIAEKMQQLIPRQMFDVAIQAAIGSHIIARQTVKALRKNVTAKCY
                     GGDVTRKRKLLEKQKAGKKRMKQVGHVEIPQEAFMAVFQTDKKK"
     gene            24535..25290
                     /gene="lepB"
                     /locus_tag="U6309_000236"
     CDS             24535..25290
                     /gene="lepB"
                     /locus_tag="U6309_000236"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN5503704.1"
                     /translation="MNFALILVILSAVTGLIALLDWLIWSKKRLPEQKPGRIIEYSRS
                     FFPVFFIVLLLRSFLVEPFRIPSGSLEPTLLVGDFVAVNKFSYGLRLPVWEKKIVPIA
                     NPKTGEIAVFRWPPEPSYDYIKRVIGVPGDKISYHDKKLTINGKEVKQTFVEYTTDES
                     SGKAVTKYKEDLNGVVHDIFVRPDAPAVDFDIVVPEGNYFMMGDNRDDSADSRFWGFV
                     PDSYLRGKAFLVWMSWNGKTDNVRWSRIGKLIH"
     gene            25302..25694
                     /locus_tag="U6309_000237"
     CDS             25302..25694
                     /locus_tag="U6309_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HEN5503705.1"
                     /translation="MRKQQGMTLIGMLFTAVVVIMAGILVMRAAPVYIQHYSILHSIK
                     ALNSTPVSSLSGDPLTDVTTLRESVTKRLDINGVEDLKPGELVITPSGPNKFKFKLKY
                     QVIKPLIYNMSLLFNFEDNFEVITGSEN"
     gene            25684..26358
                     /gene="rnc"
                     /locus_tag="U6309_000238"
     CDS             25684..26358
                     /gene="rnc"
                     /locus_tag="U6309_000238"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEN5503706.1"
                     /translation="MKINLERLCRRLNYHFNNIAYLKQALTHCSAGSDNYERFEFLGD
                     SILSFVIANELFNRFPLHSEGQLSRLRSFLVKGEMLAEIAREIGLGDYLFLGQGELRS
                     GGFRRTSILADALEAILAAIYLDGGMTAAKQIILMLYSSRLDDPDLNHCLKDAKTQLQ
                     EFLQASKFALPEYVLTKIEGDEHAQIFHVTCTIEGVSQVAYGTGPNRRKAEQLAAKAM
                     LEQLQG"
     gene            complement(26386..28236)
                     /locus_tag="U6309_000239"
     CDS             complement(26386..28236)
                     /locus_tag="U6309_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN5503707.1"
                     /translation="MLTLRQITLSRGDKILLDKVNVSLYEKQKIGLIGHNGCGKSTLF
                     DFLLGQLSPDTGEYLINPQLSISHLSQHIPDSNEKALDFVLAGDQQYIKLLKRLSEAE
                     KNGDDAEVLQCHEELSHTGGYSKPAQAAVIMSGLGFSGNQQQEPVNSFSGGWRMRLNL
                     ARCLMKPADLLLLDEPTNHLDMEAIFWLERYLKQSPSTIILISHDREFLDAFVTHILH
                     IENRSLTLYSGNYSSFEKIHAQQLALQQAMHEKQQTKIKHMMTFVTRFKAKATKAKQA
                     QSRMKAIEKMEIIAAAQADSPFSFDFFPCPRAGNPLIQCNQVDAGYINHPPILKKIKL
                     SLNPGDRIALLGPNGEGKSTLIKTLTADLPVLSGDIHRSPHLQIGYYAQHQLEQLDCK
                     LSPVETLQTLSPEVREQTIRDFLGGFNFRGDMAVQSIQHFSGGEKARLALARLVWLKP
                     NLLLLDEPTNHLDLGMRSAIELALQTYEGALILISHDRHLLKSSVDNFYLIYQGKVQP
                     FEGDLDDYYLWLQTRNSEKESKLATSPNEYKERKSYQNKLKKLEQLIEHYQNQLRLLD
                     KQLADVSLYEDTKQKSKLDNLLMERDIAHSALIKTEEEWLEICHALESLE"
     gene            complement(28359..29585)
                     /locus_tag="U6309_000240"
     CDS             complement(28359..29585)
                     /locus_tag="U6309_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN5503708.1"
                     /translation="MIMKTKLLPLFDNLNHIHKNHENVILPDVRFLNDFTYCLSFLKS
                     YIGSEGTFNSYRRETERLLQWSWLVKNSTIDSLRRDDIEQYIHFCQNPPKTWISLKKV
                     PRFIEKNGKRIPNEEWRPFVVTLSKVSRKNGHLASIDQFKLSQGAVQEIFAILSTLFN
                     FLIAEEYLISNPVALIRQKSKFIRKRQHNAPVRRLSLVQWEAVLRAAESLAEDSPEKH
                     ERTRFILSLLFGMYLRISELAASERWVPSMNDFAKDSNGHWWFTTVGKGNKERQIAVS
                     DAMLESLTRWRLFLGLTPLPSPADNSPLLPKLRGHGPMSNTAPIRRIVQHCFDLAEEQ
                     LRLNGLSEEADNLSAATVHWLRHTGISEDVKIRPREHVRDDAGHSSSATTDRYIDIEK
                     QARYQSARKKIIQPSE"
     gene            29836..29912
                     /locus_tag="U6309_000241"
     tRNA            29836..29912
                     /locus_tag="U6309_000241"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29870..29872,aa:Pro,seq:tgg)
     gene            29918..29994
                     /locus_tag="U6309_000242"
     tRNA            29918..29994
                     /locus_tag="U6309_000242"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29952..29954,aa:Arg,seq:tct)
     gene            30155..30230
                     /locus_tag="U6309_000243"
     tRNA            30155..30230
                     /locus_tag="U6309_000243"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30188..30190,aa:His,seq:gtg)
     gene            30321..30405
                     /locus_tag="U6309_000244"
     tRNA            30321..30405
                     /locus_tag="U6309_000244"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30355..30357,aa:Leu,seq:tag)
     gene            30435..31766
                     /locus_tag="U6309_000245"
     CDS             30435..31766
                     /locus_tag="U6309_000245"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEN5503709.1"
                     /translation="MQVSVETLEGLERKVTVSVPTEKVEEEVSSRLRNLARKVKIDGF
                     RPGKVPFNVVKSRFSDSVREEVAREMVQSTLYEALQKNELVPAGYPHVEPLEIEPGKD
                     FKYTAVFEVMPVFEIVELNQAPVELIRSEVTDKDVDNMIEKLREQNKEWHEVTHAVKK
                     GDKVVIDFQGFLDDKPFQGGSAEGHELVIGSGSMIPGFEDGIVGGKIDKPFDIKVSFP
                     EDYGHKDLAGKEATFKITIKKIMEGKLPALDEAFAEKFNIKEGGIESLKKDIRENMAR
                     ELERRVNMMNREKLFDSLMSVNHVELPIALIDKEIEHLKHDMYHRLFGHEHKDDEKIP
                     DFPRELFEEQAKRRVHLGLLFAEYVKKHEIVADNDKVNAMIDKFASAYESPDELRAWY
                     QSSKEHMAEVEALVMEDMVADKIAEDAKLKYKNMDYDSVMNPKKGTEKKGE"
     gene            31769..32413
                     /gene="clpP"
                     /locus_tag="U6309_000246"
     CDS             31769..32413
                     /gene="clpP"
                     /locus_tag="U6309_000246"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEN5503710.1"
                     /translation="MPGYSDNIIRNASGLIPMVIEQTSRGERSYDIYSRLLKERIIFL
                     LGEVEDHMANLVVAQLLFLESENPEKDISLYINSPGGVVTAGLAIYDTMQFIKPDVST
                     LCIGQAASAAALLLCAGAEGKRFCLPNSRVMIHQPLGGYRGQATDIEIHARETLAVRE
                     RLNNIMAKHTKKTPDQIMRDTERDNFMSATQAMEYGLIDKVLYDRQVAGHSTDL"
     gene            32554..33828
                     /gene="clpX"
                     /locus_tag="U6309_000247"
     CDS             32554..33828
                     /gene="clpX"
                     /locus_tag="U6309_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEN5503711.1"
                     /translation="MSKSGNGNGDKVLYCSFCGKSQHEVKKLIAGPSVFVCDECVELC
                     NDIIREETHETHEETEARLPTPKEISNFLDEYVIGQQHAKKVLSVAVYNHYKRLQHKS
                     EDGVELGKSNILLIGPTGSGKTLLAQTLARILNVPFAMADATTLTEAGYVGEDVENII
                     QKLLQKCDYDVDKAQQGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVA
                     SVPPQGGRKHPQQEFLQVDTSNILFICGGAFAGLDKVIRERSDKSSIGFSAQLKSKKS
                     SNDEASKVLGQLESDDLIKYGLIPEFVGRLPVVTTLQELDEAALIDILTRPKNALTKQ
                     FQSLFKMEGSELEFRDEALIAIAKKALERKMGARGLRSILENILLDTMYDLPSLEGVN
                     KIVIDESVVNGLSKPILIYEQDEKKSASGSKD"
     gene            34062..36512
                     /gene="lon"
                     /locus_tag="U6309_000248"
     CDS             34062..36512
                     /gene="lon"
                     /locus_tag="U6309_000248"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEN5503712.1"
                     /translation="MSNENEIISNETVKSSALPVLPLRDVVVYPHMVIPLFVGRGKSI
                     KALEAAMIDNKQIFLVAQRKSAHDDPGPEDIYQVGTVSSVLQLLKLPDGTVKVLVEGE
                     QRARVKEYTQDKGYLEATLEYIEEVGSTIQEQEIGILMRSLMSQFEQYIKLNKKIPPE
                     VLSPLAGIEEPGRLADTIAAHLTLKVDDKQELLETMDVGTRLEKLMAAIENEIDLLHV
                     EKRVRGRVKRQMEKSQREYYLNEQIKAIQKELGEMGEEGNEIEQLENSIKKAGMPKEA
                     KEKSLAELHKLKMMSPMSAEATVIRNYLDWMLMVPWKKRTKIQFDLLKAEKLLDKEHH
                     GLEQVKERIIEYLAVQQRVKRLKGPILCLVGPPGVGKTSLGQSIANATGRTFIRIALG
                     GVRDEAEIRGHRRTYIGSMPGKIIQKLCKAGVKNPLIMLDEVDKMAMDFRGDPASALL
                     EVLDPEQNHTFSDHYLEVDYDLSDVMFIATANSLEIPAPLLDRMEVIRLPGYTEDEKV
                     SIAEKYLVPKQMLLNGLTNEEIHISEGAIRDIIRYYTREAGVRNLERDIASICRKVVK
                     EILSSKKGKKGKTISITVSNITKYLGVKKYRYGLAEQYDQIGQVTGLAWTSVGGELLT
                     IEASIMPGKGKVTHTGQLGEVMQESIHAAMTVVRSRAKAFGLPDDFYEKHDFHVHVPE
                     GATPKDGPSAGIGMCTVLVSIVTQIPVKADVAMTGEITLRGQVLPIGGLKEKLLAAHR
                     GGIKHVIIPEENVKDLEEIPDNVLRKLTIHPVNTIEQVLELALQRNPWVEEPTNIQPG
                     TILNKRSKKIKNNDLHAH"
     gene            36641..36919
                     /locus_tag="U6309_000249"
     CDS             36641..36919
                     /locus_tag="U6309_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEN5503713.1"
                     /translation="MNKSELVDAIASGSGLTKADASRVLETFMATVTDVLKKGDQVVI
                     PGFGSFSTGNRSARTGRNPQTGKTIQIKASRVAKFKAGKNLKEAVQQA"
     gene            36927..37002
                     /locus_tag="U6309_000250"
     tRNA            36927..37002
                     /locus_tag="U6309_000250"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36960..36962,aa:Val,seq:tac)
     gene            37009..37085
                     /locus_tag="U6309_000251"
     tRNA            37009..37085
                     /locus_tag="U6309_000251"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37043..37045,aa:Asp,seq:gtc)
     gene            37177..39051
                     /locus_tag="U6309_000252"
     CDS             37177..39051
                     /locus_tag="U6309_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEN5503714.1"
                     /translation="MLQKLNEHIQGVVAWLVIILIAITFTLFGVDYYFQSRQISDAKV
                     IVNDKPITMQAFETNYRRTRAQQDLPQMTAADEKNLQNQVLNQMITNEVSIQAARKYG
                     FEVSPEQANAAIVQIPQFQEDGHFSAQRYQQALSGALFTPETFQNEVRQGMLLNQQRF
                     AFMGTSFALSDEIKRFVRLYMQTRDYDYLTVPSDRFEQQAKISEDDIKSYYNQHHKKF
                     MTSEQVTLDYVLLSMHDIKSQIKISPEEIKNYYEENKNNYLTPAQWQVAHILFAVPEN
                     ATKEEQDSIKQKADEVYSDLKKHPEQFDKYVASKSDDKLSIANKGILPWITGGQNEYG
                     RVLSNLTRPGQISIPVQTKYGYEIFKLIAYKPVTTKSLQEVENIIKDQLTSDMAQAKY
                     AQVLEQLTDLSYQTPDSLDPVLDSLQLKIQHTEPFSRDGGKQPITKNKQVIHAAFSTD
                     VLELGNNSEPIQIDNDSVIVLRVKKHIPSREQTLDEVRNQIKEILAKQYGDAKAKEIG
                     SSLLHPVEDKQQQELIANNQFEWHSVENASRDSDKANSLINDLAFNLLRPESRDGVIL
                     DNGDYVVVKLKRINDGKLSSLDQEQRDSLIQQIEASYGMMDYDLYVNNLLHHANIVRK
                     "
     gene            complement(39596..40402)
                     /locus_tag="U6309_000253"
     CDS             complement(39596..40402)
                     /locus_tag="U6309_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEN5503715.1"
                     /translation="MGGDTIVGFLTGKKALIVGLASNRSIAYGIAKAFHNQGAELAFT
                     YQNEKLQSRVEAMASEFNSTLVFPCDVASDEEIKAVFDNLRNHWDKLDILVHSVAYAP
                     ADQISGDFVECANREGFRIAHDISAYSLIGLSQAALPMMLDTQGSILTLSYYGAEKAV
                     PNYNVMGVAKASLEASVRYLAASLGSRGLRINAISAGPIKTLAAAGIKDFRKIHAAYA
                     NITPLQRNVTADEVGNTAAFLCSDLASGITGEVLHVDAGYHAVSAMSELG"
     gene            complement(40498..41136)
                     /gene="udk"
                     /locus_tag="U6309_000254"
     CDS             complement(40498..41136)
                     /gene="udk"
                     /locus_tag="U6309_000254"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEN5503716.1"
                     /translation="MTKQAIIIGISGPSASGKSLLANTIVNELGSEQVVVISEDAYYK
                     DNGHLPFAEREKINYDHPDSFDHALLYEHLRQLRVGNTVQIPIYSHSKHLRLPETRAV
                     GQHAIIVLEGILLFSDKALREIMDIRIFMSTPLDVCLTRRLKRDVVERHRSFESVIHQ
                     YETTVRPMYMQFIEPSSRYADIIVPRGGENRIAIEMIQAKMRELLASHTRGS"
     gene            complement(41189..42826)
                     /locus_tag="U6309_000255"
     CDS             complement(41189..42826)
                     /locus_tag="U6309_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEN5503717.1"
                     /translation="MKKISCSILLLINSLISYADSGQPKLILQIVVDQLRGDLIYRHH
                     KQFGPSGFNYLLAHGLDYHNAHHPHANTTTCAGHSTIATGSYPSLHGIVDNDWYDRKT
                     RKLKYCMEDLQAKILPTLHTHIEISGRSPRNLKPSTLSDEIILANKGKAFGVSLKDRA
                     AITLAGHAGKAFWFDKTNGGFITSNYYYSSYPLWVENWNKHYQPKAYDWNLSHPKSFY
                     QNANTLPFRHNYGSFGQIFPHHVTNPPSEEYFKSLSRTPKADELTADFAKQLLVNEKL
                     GKTTGQTDYLAISFSAVDAIGHQFGPNSLEAEDNLIALDDTLSQLFKTIDKEVGLNNT
                     LIILTADHGVSDGPSYLKAHKIPEIKPVNIEATGKHIENLLLKRFNLPAQALMSINPP
                     FIYLDHQIIKEKNLDIAEVSFYLAEEISHLPGIFKAYPLPIRDIEKDWLSAKVDRMSY
                     AYRAGDVYIVQPPYQSHGAKSEDRVAHGSPWQYDTYVPLLFVHPDLKPKRIFRQVHTT
                     DIAPTLSSLLLIKPPAAAVGQPLVEVVNAFHKTGEND"
     gene            43196..43858
                     /gene="lem14"
                     /locus_tag="U6309_000256"
     CDS             43196..43858
                     /gene="lem14"
                     /locus_tag="U6309_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem14"
                     /protein_id="HEN5503718.1"
                     /translation="MSFELVAYEKLKGSIRESIITLIKSHNEKAKIIEDKLEDSVKEV
                     SRERQPQVLVLLKTIELLDNSSKEPEDKARVLNALAYYIRDQIAATYKYTSPDNSDFY
                     KSLTISLDLNKDNNPNREDLADMYSALEKFLRSHVYKNSDPRKGYLDKQPFAIKHYSV
                     VDDILELSDRVHKLRREIIIAARDLHLLQQKPKSSQGGLFSVPSGKEKVTDTNEVVNH
                     RL"
     gene            complement(43892..44242)
                     /locus_tag="U6309_000257"
     CDS             complement(43892..44242)
                     /locus_tag="U6309_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN5503719.1"
                     /translation="MTKHKIKTIDVHELKHQMDNQANLSLIDVRELDEWEMMHIPGAL
                     HIPKDRISIEIQAQIPNKEQTIYLHCRSGVRSLYAAQCLMDLGYCEVYSVDGGIMAWA
                     MSGYPVKQESYTPS"
     gene            complement(44229..44510)
                     /locus_tag="U6309_000258"
     CDS             complement(44229..44510)
                     /locus_tag="U6309_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503720.1"
                     /translation="MLSELAKQFSAQIQTFFYLIMLINGLLHFIFAGAVARDAGNLYR
                     LGQKPVLVSAPTWAFATLIGGVITATIYWLLHHSTLTRPTMREIRYDKA"
     gene            complement(44514..45410)
                     /locus_tag="U6309_000259"
     CDS             complement(44514..45410)
                     /locus_tag="U6309_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEN5503721.1"
                     /translation="MLHLKTIQSILLLIVLGFIWGSGYTLAKFAMTNGVNPLGYAFWQ
                     SLGPALLLTLVSFFTKQTRLLHPVYWPYFFICGLIGIAIPNTNMYFIASHIPAGLLAV
                     LVNTVPLLVYPLALMAGQEKPDGWRFFALLLGMSGILAIIGINSAGLYSSWTILAMLS
                     PLGFAFCSIYIGAKQPKEINALQAANGMLLAASLLLTPVVLKQHAFYSLSAPLTLVKQ
                     IVILEVILSSLGYLLFFILIRMAGPVFYSLTGGVVAITGLFWGYLAFDEKPSSIQGIA
                     IALVVSAIFLLSWRQSRQTQGI"
BASE COUNT        14017 a         7932 c         9361 g        14297 t
ORIGIN      
        1 ggctagttag tcgcttgcgc gaaatagcta ttacaaagta caattaatga tcatatcgat
       61 tcttttgtga taaaatctgc cctctttaat agtctagcaa agtgaagcga tgtgttcaaa
      121 aaaaagcata tagtaaaaaa tgattcggac atggattatg tcgacgattt tataagtatt
      181 gaagaacaca gttcaggaga tgatgttact aatgaacttt ctttagcaga acaagtattt
      241 acccgattgc aaatctactc agaaggattt aagccgagct tttttataca caaggattta
      301 gcgaatctta gtttaacctc taagacattg aatggtgttg tatgttccga gttgaatcaa
      361 cgagcagctc agaaactctt aacccacgta gttaagggag aacaagataa agcgcaagcc
      421 atgctagaaa aaaatccagg attgctctta attaaatcaa aagcggtaga ctattctgga
      481 agaaccattg taggaacagc ttttcaaggt gcaattggtg cgggagataa accgatgtgg
      541 aagatgatgc tttcttattt tgaaggctta gaacaaggag aagccttaag gcagtttgat
      601 gaacagtttc ccagtgggac tgaagaaact cctgccgctg agctaaaagc ctattacgat
      661 gctattgcat cagccattat ccatgatgaa gaccatggtc tctccgtaat tgaaggattt
      721 agaaaagaaa taacctgcca aaaggaaatt acgcaaggca agcattttaa tctgcagcac
      781 ttaatcgcag cgtatcaggc ctacatcgat aacttttatg ctttcggtaa ttggggcaac
      841 cgtgacttat tttggcaaaa agtgattggt tatgtgcagc ggcagatgac cgcttatgac
      901 gcccaaattt attgttcggg tgtaatcagc gtattatata acgagagttc attcagtagg
      961 acgtttgagt tgtctgatga taagaatttt tttcccttac agattgattc tgctctcggt
     1021 ttcgattttg cttgttatag ttattattcg gcccgcgctt tggattccgg tagccaccac
     1081 gtgctacctt ccccgttgaa tgaatactgg atatggattg aaaaattatg tggagaaaaa
     1141 acagacgcac ttgttgggct tagagagtcg cttgagcaag gggtgtatta tcagctcaaa
     1201 taattgtttt ttattgagac caaactgagc attaaacaga tccttagcta aaattccaag
     1261 tcctgcttga tgggtggttt gaataaactc gattaatagc tgctgataag accattgagc
     1321 atttgttttt acccaccagg aacaccactg ggacagatag tttttgatcg cttggatagc
     1381 aaacccatga gtttactggt agaaacgatt cttcctgatt taaagaagac aaaaggagga
     1441 tgttggtggt tgggcagaaa actaagagtt catattcatt tgggtgtttt tattacagca
     1501 actgtttaat aagacagatc cgcaaattga atatgatgtc aaagataacg cggcgtacca
     1561 acttttttgt ggtataggga ttgttgaaca atgacatatt cctgatcata caaaaattga
     1621 agagtttcga tcacgattaa gtgaagaaac tcaagaaacc ctagaaaatc tgatggcaaa
     1681 gcaagcagtt ttgcttggat ttaacatgag gcaactcatt aagtggcaaa aatttcctgt
     1741 caggagaaaa atcgcatgaa tcaatttcgc gccagagact aattacaaac gatcatactt
     1801 tatatttaat gtaatagttc ttggttaact ggcacatagc tatagagccg gaaacagcaa
     1861 gcatgatagg gataagaaag ataaaattaa tcctggtgaa ttcgaaaatt aagataatgg
     1921 cagttaaagg cattttttgt gccgcagcga gaaaagccgt ggcaccaata agcgcgaagg
     1981 catggatggg agcgggtggt aaatacaaat tccaaattcc accaagaata gctcctaata
     2041 aggcgccgtt agccaatgat ggtgttagta agccgccttg ggctcctaca cgcaagcttg
     2101 accatacgaa tacattcctc aacataaata gtataatact taaaccaatt cctactgatt
     2161 gatcaaactc catttctgcc ggacttttgc cgttacctag taaagaagga aaataaattg
     2221 cgaataagcc aatgattaaa aaattaatta aacaagccaa tatcatttgc caattatgca
     2281 ttgctttgct gcgttgtatt gtagcaattt gaataaacca aaatgcaaaa aagcctaaca
     2341 ccgggctggc aagaatagac caaataatta aaggcgcact taatgaaaat tcagggagtt
     2401 gataaatcgc ttcatttccc aaaccccacc aagacaccag aactgctata gcagaagaag
     2461 ataatgctgg aagcaagaca gtccagttgg aagtgcataa caacacttcc aaaacaaaaa
     2521 cagcaccgcc taaaggcaca ttataaactg cagcaagccc cgcgcctgct ccacatgcca
     2581 acatgatctt gctttcctca ggagttaatc cagctttgct agatagccag taagcaaaga
     2641 cagagcttac ctcacgagga gccacctctc gtcccagagg tgagcccaat gcaattgtta
     2701 tgatttgtaa aaaagcatga atgaatgtac tgagaactgg catgggttta cccgatttta
     2761 ttgccgtcgc aatactgact aatggtttac caaagcgata aagtgcccac catcccatgc
     2821 ctgcgattaa gccacaaagg aatagtatgc ttattctccg ctcagatgaa gaggctctaa
     2881 ctccctccag aaaagtttca ttactgatga tgtgaagggg gctgtaacca taggcaatat
     2941 gttgaagata atgcaataac atggccaggc acatgcctag aaaacctgaa ccgattccca
     3001 tcagaacagt aaccagagcg agacttactt tggaactttt aaatagttgg ttctcggttg
     3061 tggttaactc cattccctgg attcctttaa ggtaacttgt catcaatttg cttatagggg
     3121 taagaatttt attatttctg tatgcagatt tttttgcaag tgaaacagat taataaatgt
     3181 agccgtaata acaaaattta aaaaagtatc cattgtttgt ttgcgatctt gacagcacat
     3241 tgcctaaaaa gttaacatta aacattcttt taatttccag ttttttatga atataagacg
     3301 acaattaata agcagaaagt cttgttccca aagctgcaac agtcagcctg ttggattgtc
     3361 gcctattgtt ttaaactctc tcatctcaat tgtcgtcgtg gtggtggtca gctttaccct
     3421 ttcttctgct ctgagacggc ttctctcctg acttatctgc cccgcctcag ggcgataaca
     3481 gcagcagata agacctggcg gtttttgaaa ttttatctct aacctgaagc aaataagtta
     3541 ggagttattg tgaacacatt gtcattcaag catccattgc tggctaaaac ttatctttgg
     3601 attatatgga ccatcgcggc atgttttttc ttttataaat atttgattca ggtttctccc
     3661 agtgttatga cagctgactt gatgaaggca tttcaggtta atgcggctgg tcttgggaat
     3721 ttgtcagctt tttattttta ttcctattta tttatgcaga ttcctgtggg ccttatgtta
     3781 gacaggtaca gtccaagact gctgacaact cttgctattt ttgtctgcag tatcagtact
     3841 tatatttttt ctcaaaccaa tactttatgg ttggcctgtt taagtcgagc attgatgggg
     3901 gcgggagctg cttttgctgc ggtgtcttgt tttaagctag ccgctgtctg gttttctcca
     3961 aaacgatttg ctttagtatc agggatgttt atgacagcag ctatgctggg tgctgtggga
     4021 gggcagatgc ctttggcatt cttggttcaa catgaaggtt ggcgcaaggc gcttgaatta
     4081 gttagtctga tgggaattgt cctgggggtt gtgtattttc tcattttaag agataagcct
     4141 gcacaaacgc agcctacgca aaatagcaag gaacctgttg ttcaattgtt aagacgtatt
     4201 atcgttaata agcaggcctg ggctttgtct ttatatagcg gattagcatt tgctcctgtt
     4261 tcggtttttg gtggattatg gggggttcct tttttggaaa aggcctatct cttatctcgt
     4321 actgatgcag cccttgcgat ttcatttatt tttgtcggtt ttgcggcagg cgcgccattt
     4381 tggggatggt tttctgattt tatcaggcgg agaaagcctg ttttgtttac tggaacgtgt
     4441 tctgctctat tgtgtttatt ggtagttatt tatagctcaa accaaaattt gcttacttta
     4501 attattcttt tatttctctt tggttttgga gcgagtggat tttttaccag ctttgcaatg
     4561 atcagggaat tatttccttt ggtacttgta gcgacagtac ttggtattat gaatacgttt
     4621 aattcggtgt ttgaggcttt gtttgagcca ctggttggtg ctttgctgga ttggacatgg
     4681 gaggggaccg tggtggatgg tattcatcaa tttactttac atgggtacta tttttctctt
     4741 ttattgttgc ctctgtcttt aattttagct ttattgacct tgttattgat tgatgaaaca
     4801 tattgtcgaa caaaggagga ggtagagtaa acctcctcat tattgattgc aggttatttt
     4861 ttgaggcagg aaaccatatg ggccttttgc gtttggatta aaaatcacag tacatccttt
     4921 tgttttttga ggcaattgtt gtgtcagttc aattcctgtt tgattgtaat gacacacaat
     4981 gtaaatggta tcatgaggag aaaacttcca ttcggctttt ttcttattga tacggtttgg
     5041 ttttaagctg gcttgacctt tggggtgacc ggaataaaag gaaacattac ccagataatt
     5101 gtttttaatc ccgctcaggg tttcccatcc atccggagat gtctgtatac gttcaagcgt
     5161 attaatttct ttagggcatt gagggatata ggtttttgct tgtaaaaaag tagaaaaccc
     5221 cattaatgcc aagccaataa tcctgttcca attgcacacg gaagtcccct tattgagtaa
     5281 acaggtatca tatcattaaa tgatgaaaat gattatctgg ttgccttcaa attgttcctt
     5341 gcaagtattg tgaactctgg atcatttttt aataattcaa atattctgta taaatttaaa
     5401 ggcaataaaa ccgaatgcaa aataaatata ggatgtaaat gataacccaa accgtaagta
     5461 atgaacgcaa cattactaca aacggctatg agtcttaatg tcagaatttt tttgacataa
     5521 aatgttgcaa agactaaaga agaagctaca tatccaacca aatcaatcat tgattccatt
     5581 ttaacctcca cttccaaatc cctttcattc aacataacac tgaccagagg agattagcaa
     5641 gttttttaat caattcttat ggttttctta agtgctgaaa tgtataatcc atgatctttg
     5701 agttaaatat attaccaagc aggaattttt tgtttatatt tggagacaat atgcccctta
     5761 cttcgttaaa aaaattttta aaagatattg gaaaggaaaa tataaaacag catcaggtaa
     5821 aacttgtttt aaaaacatct caggatattc aattattttt aaatgctttg cgagattcga
     5881 gaaaccacgg aatctcgtcc ttatctgaac ttgatctaag tgatactcgc tttactaatc
     5941 aagagttgag tgatttagtc acagctctta ataatatacc tggaattaaa tctttacgct
     6001 tggattcttg tggtcttaag gatagtgaca cagtggagct ttccaaatta actcacatca
     6061 aaaaattatc attgaaaagt aattacttaa agaaccgccc tatgtttaat tctatgcttg
     6121 aagcattgta tttggattat aatacagaac ttagcgcaag ttatgtgtta tttagtttaa
     6181 gtcgcaatgc tgccgcttta aaaaaattat ccttgcgtaa ttgtggtgtt actgatgcta
     6241 atttagagta tttgactcgg cctgagtcca ggttaaaaag tctgacacac ttgaatttgc
     6301 gaagaaataa tatcactcac caaggagtgg atagcattgc tcatcttcaa tcgttgacta
     6361 ctattgattt aagtcaaaat actgggattg gtgatgaggg tgtatccagg cttgcgcttt
     6421 taaagcagtt aagaactctg tatttggata attgtggaat aggggcagag ggaattaagg
     6481 caattgccaa aatgaatttg cagactgttg atttgagttt caatccaggg ttaaaaaaag
     6541 aatggggatt ggatgacatt cgacctaatc ataccattcg aacattattg ttaacatttt
     6601 gcagcttaaa tgataatcat gccaagctta ttgtttcgaa gtttccagcg gctaccgatt
     6661 taaatgttgc gaataataat atgactaggg caggggtaaa gactttattg agtaacccga
     6721 ttatagaaaa tttggatgtc agtacccaat cgctttatgc aaaatcaaca ggagcttctg
     6781 gcactggtat gatctctccc cgaataatga tgaagaaaaa ggaaataaaa caacaggaaa
     6841 aagaaaaagc gcaagatttg ctagatacta tttgtaatac aatcacatta aagagtatta
     6901 atctggaaca tacaggcata acctccagga tgttattgag tttaattcca gatgaaacag
     6961 atcacaaacg gtatcttaag aaaattaatg gtgtttcctg taaagagctt aagccaaaat
     7021 tagaacaaca aattgccttg agaaaagctg aaaaacaaag ttctcctcct gtaatcattg
     7081 cagtggaaga ttccaaacca agtgatttaa tgattaaatc tggcagtagt tccaagactg
     7141 aggcgcaaac cagggaagat ggcttgatag ccagtcctga tgaaaaaata aaagctctgg
     7201 aactagagaa tcaaaaatta aaggatgaat tggcaacagc tcgtaaaaga atttttgaat
     7261 tggaaaatca gtctttatct ggatccaaaa caagaaaaaa aacctcaata ccaccattgg
     7321 caatcccggt gatgtttcag gattttgcaa aaccaggaaa aaatgaaaca ggtgacaaca
     7381 ccagcaactc aataacggca atccccaaat agcacaatta ttgtaaatgt attcagcatg
     7441 actaacatcc acttggatgt tagtcatgta ttgctttact ttttcttgcc aaaaatgtga
     7501 gttaagtgac caataatggt ttcaactgca tttcggcatg ccggtgtttc agagagatca
     7561 ttgagcatca ggaaatcatg gattgtgccc agatgacgaa ctgcagttac tgtcacaccc
     7621 gctgcgttga gtttatgagc ataggcttcg ccctcatcac gcaaaacatc acactctcca
     7681 gtgattacca atgctggtgg taggccttta agttgttcaa gagaggcttg cagcggactt
     7741 gctgtaattt cctttctttt ttctttattg ggcaaatact tatcccaaaa ccactccatc
     7801 gcctttttgg ttagccaggg accttcagca aattctttat aggaagcgtt atcaaaattg
     7861 gcatcagtta ctggataaat gagtacttga taatcaattt tggggccacc acgttcttta
     7921 gcaagaagag tcatgacgct ggtcatatta ccaccaacac tgtcacctgc tgcgacaaat
     7981 cgtgaagtat ccagattaaa ttgcttgcca tgttctgcta tatatttcat ggcagcgtaa
     8041 gattcttcta tttgtgtcgg gtactgagct tccggagcta aagtgtattt aacaaacacg
     8101 actgcggcat gagtacccac tgctatttct cgtacgagac gaccatgagt ttgatagtct
     8161 ccaaaaaccc agccagcacc atgaaaaaac ataatgacag gtaaggcttc ttttgcacct
     8221 ttagggcgaa caatgcgaat gtcaacctta ccgccaggcc ctactggcaa agttttatct
     8281 tcaacatcga catcaggttt ctcattaggc aaggcttgta gttgatcaaa tattgccctg
     8341 ccttctgcaa ccggtatgtc ataaatcggt tttccacccg cattttgtat tttttcaaca
     8401 aatgaccaag tttttggttc tattcttttg cttaaatcca ttttgatttt ccttaattta
     8461 taaataggga tgacttctta tgtatagtat attgaattga aaaatcaagg aaatttgagt
     8521 tcgtttgtag ataacaataa ctggtggatg gtgtgtgatt tggtatgcaa tgaattaaat
     8581 tttgttttga gtcgctcaaa ttaatttgca aaaatattaa atcaattcaa tgatataaat
     8641 taggtaataa attaatttat gagtgaatca tgctccaatt ttttgagaaa ggtttgcaat
     8701 attcaggaga aaatatttcc gccgattctt atttaatgag aaaaataatt gaagcagcaa
     8761 ttccctctgc tgataaggga actgtttctg cagaaaaaat gcttggcatg gtgaatccaa
     8821 atcagataga aatagtatcg ggtagtgaga acgcatatcg tttaatagtt cataaaatta
     8881 aagaggctaa aaagcaaata cttattcagg cttttgtatg ggaaccagga actcaggttg
     8941 tcaaagatat ccgaaatgcg ctttgtgaca tcaaacatga agtcgaagta tttcttctgg
     9001 tcgatcagtt ggatcgttgg gcacaagttt tttatcatgg cgaaatactt cctaaaaaac
     9061 caaaacatga tccagcctgt ttgggtttag ataatttacc tcagaatatt aaattgcata
     9121 taggtactca tgttcataat tccattgcgt ccaatcataa taaagccatc tggattgatg
     9181 gggatgtcat tttgacaggg gccaattttc agcaggaaaa ttatggccct aatgggtttc
     9241 atgatgccgc catgtttatt cctggcggcg cagcggaagc ggttttttac gattttaaag
     9301 cgatgtggga taagcgcacc aataaatctg atgcaccaga aatttctccc atatatacag
     9361 gccaggagtc atcaacagaa ttttatagaa atacaaactc ttgttctgtg ctttatgtga
     9421 caaatactat caggcagaaa tgggcgcttc tcccttttta tagggcgcct ttacctgaag
     9481 atccattgaa taacgcctac atggctgcta ttaaatatgc caaacatact atccgaatcg
     9541 ctgttcccaa tttaaactcg ccggaaatca tccatgctct ggcggatttt attaataata
     9601 gaaatggcca tgtttatcta ttgatgggaa agggattcaa tgattttcgt gagagattct
     9661 atggcggtac aaatcagagt tcgatagatt attttagttc actacttgat gaggacaggc
     9721 aaaataactt gcaagttcgc tggtttcatc gtgtgaatga caatgaaatt tctatgaaaa
     9781 aagaggttat tcatatgaaa tttatgtcaa ttgatgggca ggtggttatt tacggttcat
     9841 cgaatcttga tttgttaagc ttacataata gccatgaaac aaatattgta attgataatg
     9901 ctgattttgc acaaagagca atagaacaat tatatctacc catctttgaa acgagtatac
     9961 cagtattgat atcgcctaaa agaacctgtt ttgtttgttc ttgattttag gttgttttga
    10021 tcaagtcaat attaattata atcctgatac atgagttcga cataaaaaat aattccattt
    10081 ttatccaaac tcatgcaaat taccatcaat gtctttaagt tttagatgta taagcattgc
    10141 caatgcccaa gtccacacgg gcgaagttat ataaattcaa ataacctatt ggttagcctt
    10201 ttgcaatgct tgctgctgta cttgtttaat ctgctcctgt agggttgctt gtacttgttt
    10261 aatttgatct tgtgtttgag aattgactgt ttgaatttgt tcttgtaatt gagattggac
    10321 ttgtttaatt tgattttgta attgggtatt taaagcatcc agttgttgct gttgttttgt
    10381 ttgcatttcc tgtaattgag attgaatctg agcatttaat tgctgaatct gttcagctgg
    10441 tgtgccggtc agttgagcag gggtttcatc agcataggct ggggaaaaca agattgccaa
    10501 catcaaagat ggaagtgcat aaaatatcac ttttttcatt ttttatctcc attattgaaa
    10561 aagtccaaat tatctaattt attttgcttt aataataatg ctaatttaac tatagcagaa
    10621 tcagagtgaa aacaggttat acaatgtcta atgaaatgaa gtaactgaca tttttacgat
    10681 aggtggaaaa gaatcatgac cagggaatta tgtatgcgtt gttatatttc aggaagagtt
    10741 caaggggtat ggtttagggc ttctgctaaa aaattggctg aacaattgat gataagtgga
    10801 tgggctcgga atttagcgga tggtcgtgtt gaggtttttg catgtggaaa agaagaaaag
    10861 ctggaagagt tttataactg gttgcaaaaa ggacctctta atgcaagagt tgatgcatgt
    10921 actcgagaga atttaccttg ggaagattat atcacttttg atgtcttata aaaaaagcct
    10981 attgtgattt aatttttact tgccatgcct tcgaatagat acaagctata ctcaataaag
    11041 aaggtacagc ttatggaatt aaaaagacag tgtattaacg gggtagtagg tgtttcttta
    11101 tctttattgg ctgtgttaat ccatccactc tttgctgaag atgccccaca gtttgttaca
    11161 ggccaatccc agttatcagc tgcacgagat ccggcagaca gtagttggct tcctgatatt
    11221 attggcttgc ccaatgaaga gcaagttgaa agtcttacca gacatgtcgc tgaggtaatg
    11281 acagaagagg aagataagaa ttgtactgca atagactata atctcaggat gcttgttttt
    11341 caaaattttg taatttatca gcatatggcg aatgcacagc accactattt taaagaaaaa
    11401 atggcgctga aatatgctca cgttcttgcc atgattctaa aagaaagtag cggtgatcca
    11461 actaatgtaa cagacatgaa tggccgatca atatctacaa gcaaacctgg cactaatttg
    11521 cagcgctgga gcaatttatt aaaactaaca atgcaaaatg gaattaaatt taattatcaa
    11581 accaattttg gactgactca gctgtctgcg gacaggttgt ttgttgcttt taaattggct
    11641 aatggagcga gtcatagcaa agattttctg gaagggaaga atggagatga aactcctaac
    11701 agagtagaca tggattcggc tattgccatt cgcagattga tttggtttta tcaggatatt
    11761 gctcaagggc gattaaagca aaaagatgaa tgtattaatg aggaggatat ctataaacct
    11821 gaatttaatg aaagatacca agctggctta agaatggctc ttttatattg tggtacaaaa
    11881 tttttgtttc gcgttgataa tgaatctgtt tgggtaaatg aaaattcgac ctttgaaaag
    11941 gcaatggctt ctattgccta ttgcaaactg ggcaattcaa aaacagggta tggtaccgat
    12001 gaaatggatg agcggtgttt tgcagaatgg gtaaccttat gtccagcttt gaatgtgaat
    12061 atagcactgc ttactccgct tagttatttt caaaccagaa aaaacaaacc agtttgtacc
    12121 aatacattaa atcgtttact gaataaagaa ccaaaaaatt gatagccttt gtcttatcac
    12181 tttgaacatt taaaatccta tcgttacttg ctggttacct attaatctaa ttaacagttt
    12241 tgatgattag agaacctaag tggagggaaa tttgttctca atcctgtaaa aaagataatg
    12301 cgtattgata tgctacattt tccactacta atcacagttt tggctttgaa tgcaatttcc
    12361 ctctgaatca cattgctgga ctgtctggca gttatacccc gtattgctta ttatatatgt
    12421 tggcgtagca tacactggag caacccagcc ataacctggg tgataattac cgacgcctat
    12481 aacatcgtga ttgtaataat ctggatgata ccatcccaca taacctgcat ttgtatgaat
    12541 tccaccaacg aaatgagctc tagcacaaat catagaagca aataatgcaa tgacacagat
    12601 aactgcgcga gaaatggata aattccattt gggtgtgctc atagctctct ccttttcaaa
    12661 gaatcatttc actgatgaca gtcaatattc ttaaagtata gttcttttat tattctctgt
    12721 gggtttgtgt tcataaagta aaaaaattaa ctgatttaat ataaattgat tatgggcaat
    12781 agatctaaag tagctcatta gtagaacaat tatgctgtct atgaattgct ttagtttgaa
    12841 cctatttatg ctcagtatat aattggcgat ctgttttaaa ataaattttg ttgaactgat
    12901 tggtttttgg ataaccgcac gtgataagca atcgatgtgc catagcacat cgatttaaaa
    12961 atatctaacc tgtgagagtt tgagttggga ttgtattttc ttctgatgaa acgggcgttg
    13021 gagactcttt ttcctcagta gccataatac ctaatttacc aagtactttt gcattactat
    13081 acggcgtagg cttacgggaa agttggagtt gttccaaacg gttaactact tcttcttgac
    13141 gttccaatag ttcctgataa cgtttgttac taaagtttaa ttcttcttta acaaggcata
    13201 gttcgcgctc ggcttcacat atacgttgag caacatcatt gaagctttgt tctttatttg
    13261 agagaaaatc attcaattta ttttgaaacg attccctatg attgtgatct gcaatagcag
    13321 tttgggacag tttttctaca gtatttttta atacttgggt aacagtttcg gctttttcaa
    13381 tttctactga catttctttc ttaacttgat ccagctcaga aattttttca gataattcaa
    13441 tttcattttt tgcaatttct tccctgattc tgtttaattc ttcctgattt atctgtagta
    13501 attcggtttg aacttgtact ttttcatcca gagtttcaga tgttttttcc aattcttctc
    13561 tgactttacg caaagtttgt tcagtttttt gtaattgctc agcttgtttt gttaatcgca
    13621 taatatgatc actcagttga gtaacgttga ggtttaatct atcattttct ttatgaaaat
    13681 actcaatttg ttcagcaatt tgcttgcgga gaggctcaaa agactcgata acttttccca
    13741 ggttatctgc caaacctatc atttcttctt ttaaacgatc agtaatatgc tcttcttttt
    13801 gaacatgatt ttcaagaaga agggcactac caacataagt taccagagtc aggacactaa
    13861 ctacaataag agaggccact tgaattgcaa ttccaagtgt caagatggga acaaccaata
    13921 ctattccaag agtaattttt aaccataact gcatttctcc ccagtaggca gcaacacgac
    13981 caaaaaaact gcgatttttt gccaaggaat ctataaccgc agcaatatgt tttttgattt
    14041 gatcaaaatt ttcttgggta gtttccaata ctttcagttt ttttactaat atttccgtgt
    14101 tttcttcagg gaggggctgg tctttggggg tatcctcttt aatttcatca acgcatagat
    14161 tagcttttgg agtatctgtc atgattcata ttcctttatt tccattttga ctacattcaa
    14221 cccgaaagag cttgcaatct acttctttct ggttcaatcc catgagttcc ctgaataggt
    14281 caaactttcc tgattctatc gaataatttt aagccagtca ttattttttt agagcgggac
    14341 tctgtacata acaggttttt gtcatgtgca gagtgagtga catcagagaa agaggttatt
    14401 taattttcag atttttaaat caatcagggt gttggaggtt tttcctggcg ggctgatcca
    14461 tcaaccgatt ggttaatgca atggccaggg ctgaaacaat taaagttaaa tgaatcacaa
    14521 cctgccacat aatagaataa ttgctaagtt ttgcagggtc tataaaagtt cttaacagat
    14581 gaatcgagga gatacctatc agtgaaagag ccaatttgat tttcatagct cctgcatcaa
    14641 aatggtcaag ccattcaggt tgatcagggt gggaatccaa ttgcaaatgg gatacaaagg
    14701 tttcatagcc gcccataact accataatca gaagattggc aatcatgaca acgtcaatga
    14761 gatccaatac tccaagcatg atatgggtat catccaggcc tttaagatga aaaatgagtt
    14821 caaataattc aatgatgaag cgatacacat atccggccag tatcaagatc aaaccgagat
    14881 acaagggaag ttgaagccat cttcccataa aaataagatt actgatgcca agtttgattc
    14941 gattcatttt gtcgattcac tacaaaatat aacttattga atggccttat gataagcttt
    15001 cctgaaggtt ttgttaatac ttattatact catactatgt ataatatgat catatttaaa
    15061 tcattcttaa atgttatact cttcagagaa ttaacaaagc ctatttgctt ccggcatgat
    15121 ggttatttaa actaagtttc tgattaataa aaaaataatt ttctatagtt aaggataatt
    15181 tatgaagtat ttacatacca tggttagagt ttcgaattta gaacaatcgc tcgattttta
    15241 ttgtaataaa ttgggtttga tagaagtaaa acgaactgaa aatgccaaag gccgttttac
    15301 tttggtattt ttagctgctc ctgctgatat agagcaagtt caaacttctt atggaccaat
    15361 gctagagttg acttataact gggatccgga ggattacact gagggaagaa attttggcca
    15421 tttggcatat catgtagatg atatctataa aatctgccaa catttacagg aatgtggtgt
    15481 cgtaattaat aggccaccca gagatggcca catggctttt attcgttctc ctgataatat
    15541 ttctatcgaa ttattgcaaa agggtgagcc tttaccaaag caggaacctt ggttatctgc
    15601 acccaatatt ggacattggt aacctgacaa ttgcattctg gtagtgaata gtggaaataa
    15661 tttaatcata ctgaaatagt attttgggcc aaatggcaat gttgaatcaa tcgatgcaaa
    15721 ttttaaaatc actatcagag ggtatgggaa cagcaggggg ggtggcatgg actacctttg
    15781 gactgctatt tggttctttt ggactaactg ttggcaattc ggcactgact atttcattgg
    15841 gcgccatttc tgccagttta ttcctattag tcgcaatacc tgttacttat tggtcttatt
    15901 gtaatgcgca taaagaaaat gatgaattac gacatgctat tgaagaacag gaaaatcaaa
    15961 tcgttcatta tttgacggaa tatttattgg caaccaggca agaatgtttg cttaaaaatc
    16021 ctcaaaaata tcgtaatcat tatgataagt ctgtgctggc cgaagatctt aaaaatcgat
    16081 tattaaaaac caggttaaaa cggcctgttt cgtttacgac agcacaattt cttgactgtc
    16141 tattagatga taatcataaa aactctattt tgggtatgtt aatagatgcc tgcacgatgg
    16201 ataagttggt attgaaaaag aaactattaa ctatttatcg tgatagtgtt tttcttcagt
    16261 ttgaagaact ttccttattt gccaagttaa aaacgggatt tattgcgttt tgtgccggat
    16321 ttgggtcgct ggctggttgt tctagtggtt cattgggttt gtttgcagcc ttgggtattt
    16381 tcccctgttt tgctgcagtt cctttggttg ggttggcaat tatgggtatc gcttgtgttt
    16441 ttggtttgtt gctgtctgga atgagtattg ataccgcaat ccaaaggagt aaagaaaaac
    16501 agttatttct ccaaagcgaa gcaatccatc atgatttaaa aaatttggcc acgttgaaaa
    16561 ctgtatctgt taaggccgaa ttaaaagcac aaaccaaata tcaaattgga gctaaagagg
    16621 gattgacaga gaaattagca aggccgaagt tgctgaagcg atcttcttca tttgatagtt
    16681 atttttatag aaaccatccc ttatggaaca tttctcataa tcctccagag gcttcgaaat
    16741 gcagctttga aaacctgggt tctccttttt cactttgaat aacctgtctc tttcattata
    16801 ttgctgcgtt gcaaactttt tctggtactc acccttaaaa caaaaaacca ggttatgtaa
    16861 agatttatta caacctttgt tttcgtgctg gtcaaatatt ttttggatta gactcttacg
    16921 gtcataaatt tttgctgcaa tgatgctttt actcatgttc atttctattg ataaaattag
    16981 tgctgtgatt tggatttatt tatgattttg atgtaaaaaa aggttgtctt ataaaagact
    17041 tgcattaaaa tagaaaaacc acttgacagt attaatgcgt ctaattaaac tgcacacctt
    17101 attttggggt tatagctcag ctgggagagc gcttgcatgg catgcaagag gtcggcggtt
    17161 cgatcccgcc tagctccacc atcaacatcg ttgtatgttg atggtaaaaa aagtactagg
    17221 tccccatcgt ctagaggcct aggacatcgc cctttcacgg cggtaacggg ggttcgaatc
    17281 cccctgggga cgccattatt tggcatttaa acagtgtagt agtgtgttga tgtaatccta
    17341 ataagatcca ggtccccatc gtctagaggc ctaggacatc gccctttcac ggcggtaacg
    17401 ggggttcgaa cccccctggg gacgccatta ttttgaatgg ctattatttt aaggtctttc
    17461 ttgctatcta aagcctttca aaaattgttc ttgtattgaa tccgaataat ctctcatgca
    17521 ttcgataagc atactcgtca gtcatgtttg ctaaataatc acaaacgact ctataagcag
    17581 cgacctcatc tggagcttgc gaaaataaca ctcgattttt attatcaagc aagctggctg
    17641 gattggagct gatggcatca aatagccgga gtactaccgt ttgacctcca tactcaaagg
    17701 tacgtgcttc ttgggagtca atgacattat tatagataca ttgcattaaa tagctgagga
    17761 gggcatgggc ttctgggata agtgcaatat tatgttttaa taaactgttt tcaaattctt
    17821 cattggttac aatgatttga gtagaggtaa taaaataatt gaccatttct ccgatagctt
    17881 gttttcttaa acagatttct ggagaaaata gagaattaag caaacgctcc tgatgtagcg
    17941 agagggttgt ttcactgatt aattgcctaa actcaggagt atcaagatgt gaacggttaa
    18001 ttaatcttaa gtgaattgca tcttccaggt catggactcc ataggcaata tcgtccgctg
    18061 tgtccataat agagcaatca aaattatgat aggctgcttt acctgcttgg gtgccattgg
    18121 gctttacgga gagagactga aataattctc tgtcattatc tgataaaggc gataataacc
    18181 aatcgacttc tggctgctcg caatcaaaat aagctttagg aggcagccag tcattgattt
    18241 tgattgtttt atgaatcgat tcatgaatag ggggttgttt gggagctacc acattagctc
    18301 gttttaccgg gtattttaaa atacctagca gcgcacgacg agttagatcc aggccaaaac
    18361 caccgtaact attttcaact tttgtcagta agcgaagagt ttgtccattg ccttcaaatc
    18421 ctccataatg tcgcatcata taattcaatg cgacttctcc tccatgtccg aagggcggat
    18481 ggccaatgtc atgaagcaaa caaatcacgc tgatgagatc atcatttggc aaaaggctgg
    18541 acaataacag ctggtgttcc tgattggtca tcaagttgcg aacgatactt ctacctatag
    18601 aatctacttc cagggaatgg gttagacggg ttctatgaaa atcgccctca tctgttccga
    18661 gaatttgtgt ttttctttgt agtcttctaa atgctggaca gtgaattact cttgtcctat
    18721 ctcgctcata gggatctcta tgatcttgag agcctctttg attggtttga ccagaacgtc
    18781 tatgagtcca catatctttt gcccttattc cacaaactaa ttactttatt ctataccatg
    18841 ttgggaaaca atagagtctt tgcatagtct ctggaaaatg acaattgttt ataacggttt
    18901 tatttttttg atccacgcag aataacggaa attttttcgg acgatggctt gaatagaagt
    18961 ccaataactc tgaatcaact cataaaatat tttcctaaag ttttccttat ttttgtagat
    19021 aacaacaaat aagagtaaaa gtaaaagtta aaatcagggg ggtgttatga tgggtattag
    19081 catcaggtac atcattctag gtatcggcgt aatgaatatt tcttcttgca tggtttatga
    19141 ggatacatac actacccgct atcaaattta tacctatgat tatggtcagt tatatcctca
    19201 agcagattat catgctcgtg attacaatta tggtaatcaa tctggtcaaa gtgttgtggt
    19261 tcctgattca tatcatgttg gtgagtatca ttctcctgta tcctttaagg acagagatag
    19321 aaattgggta aacagtcaaa atccccaagg ctatacgatt cagattgctg atgacgaaaa
    19381 ggcttcttat gtggcccaaa aactctacaa agcacctaaa aatgatagaa tggctcaagt
    19441 gaaatattat caaaatggca aagcccatta taagggttta tatggtactt atgatagtca
    19501 agaagcagct caaaaagcat tggaatcatt acctcctgaa attaaacagg gtgctggaat
    19561 taaagattgg gggagtgttc aaggaaattt gaatgaatag gttatcttga gaaactatat
    19621 gtatttaaaa aaatactttg atattctgta ttttattatt ctaaggtcag ggcttatagt
    19681 cttgactaat ataatagcaa ttctaacttg tagagagtat gatcatcagt cgggttactc
    19741 ccggtgttga ggttttctcc acatcaaatt gttttatatt gatagaatag acttcgttga
    19801 tttttgcaag ccattgtata aagttttgaa aagggactgc gtcaaaggtt aattgaacat
    19861 cccccgagct tgtttgttgt aattgatagg ggaatttgag tgttttatta tttttaagtt
    19921 gggaagcaag aatagttaac aattggctgt tatctacaga ttcctttctt ttggtagagc
    19981 gtttttgcat tcttacttgc ttcatccatt ccaatgtttc agttttttca attaattgag
    20041 tggatttttg atttacctgg ttgctcagag gggcgtaaag taataaatag tagccataaa
    20101 taaaaagtga tacaccagcc actataacca tccacttttc tcgatcattc agcgtgttta
    20161 aataggtttt cacgttaact ccagagttgc aaccacttgt tgatctttcg ttgaggcttg
    20221 agtttgtttc actttgagtt gtgattgttt tagtttattt tcaatttttt gtaagctttc
    20281 aaaatcagaa cttactaatg tgattgatat tgttttgttt tgatacctta attgctctac
    20341 tgtgatacca ctatctttta tggctttgga taattgattt aatataaacc aaaatcgagt
    20401 ttgattttct gcagtactcc ccccaagaag ctgacttatt cgaaacttag gactgatgac
    20461 ctgtttggca tcaggaaaaa actcacgata aataaccgcg gtttgccggt caatttcatc
    20521 tatttgtcta ttgagccaat gtaaagaaag ccaattaaca aaaagaatag aaattaacca
    20581 gaagccgcat aatccgccaa ccaataggta tcctttttta acccaatcgg ttgtttttcc
    20641 atgttgtatt tcaccttgac aaagattgag tggttttgat tttatcaatc ttttactaat
    20701 ccaagtgtat gagtgttctt cattttggct tgctggagag gcgacaggta ttttgctgtc
    20761 ttgaaacaga tgagcgggag tgtgtggatg atttttcagg taagtttgag ccaaatcacc
    20821 tgagaggaca cctttaaaat cttcgttatt gatcaatagc tcggattcac taatgatcaa
    20881 ttcttgagat gctagcgcaa accagtcaag agttactgca tcaaactcta tcccatgctc
    20941 atcaaaaact tgaattaact ttctcattcg ttgtttatca atcacaacaa cgagatattg
    21001 gttattctga taataagatt tatcaaaggc aaaatgaagt tcttcgactg gttgagttac
    21061 tttatcctca agtgcatagg gaattgcaat tctggctttt ctttcaggta gccatggaat
    21121 cgcaagacca agaagtattg ctctggtaca ggtttctaca ataattgtac tggattcttt
    21181 ttgtaaatct tttatttgcg agaaattacg aaattcaggg ggagcaatga gctcacccaa
    21241 gtcagatatt ttcaggcata cacaaccatt atcatcgaga tgtttgctaa aaataaaaca
    21301 cttatccatg agtcaaactc tgtattgtta tggctgtcaa tttttgagta tataccagta
    21361 attattatta tttccagctg gcattaaaca taagccctgt gccttttagt ggcactagta
    21421 taccaatgtt gcttcaattg ctgggataat aaataaaccg ccagagcata ttgagggctc
    21481 gaattatagc gggtaattac aaaaaaattg ggataggcaa tccagtattc tttacccttc
    21541 cttgtaacta actcaatgac tcctgcacga ggaggatgat tttgtgacgc tgtaattggt
    21601 tttactccat agcttataag ttgtgagtat cggtaatttg ctgttttagg gttcatacga
    21661 agttgtttgt atcgccatcc ggagattttg gctggttgag cgatgccttg atttaggttc
    21721 caaccatgct tatgcaaata attggcgatg ctggcaatag agtcaccatt gtcattgact
    21781 aagtctcttc gaccattgtt attaaaatcc acagcataat atcgataact gcttggcata
    21841 aattgaggtt ttcctatggc acctgcataa gaccctttgt attgagttgg ggatacgtgc
    21901 tgttctcggc acaggaggaa aaactccttt aattctttag taaagaatgc agaacgtttg
    21961 gggtagttga aagccagtgt agccagagta tccaagactc tgtattcacc ctgacgctca
    22021 ccatacattg tctctactcc taaaatagct ataatgatct ccggagacac cccatacctt
    22081 ctctgaattt tttccaatgt tttttgattt tcagcccaaa attgcaatcc gctttttaat
    22141 cttttctgag taagaaatag ttctcggtag acatcccagt ttttcttttc atagggcttt
    22201 tccatagatt cgatgacttg aggctgaagt ttaacttggt tcattactgt agttaactga
    22261 ttacgattaa agtgataatt cgttaccatc atgttaataa acgattgtac gtctttacgt
    22321 tttgtaaatg ttgtgtcgga ataggtagtg aaagataaaa ggaagagtga aatgaaacct
    22381 agccagatga tttgtcgcat catgtaccct gtatcgttgc aaaccacgaa ttttagtaaa
    22441 aataaagcct catagcaaat ttttggcctt tgtttatctt aaatacagtg catgaaattg
    22501 gtaaatgaat aagtaatttt cattcgctta agaatataaa aacgactgaa tttgctttgc
    22561 ctactttctg catgcgaaca tttaagatag acttatgcta tttgcaagaa ggttagaaca
    22621 gttgaaagat ttaaaccgaa ttcgaaattt ttcaataatt gcccatattg atcatggcaa
    22681 gtctacgttg gctgatcgat ttatccaaat ttgtggcggg ttgactgagc gtgaaatgag
    22741 ctcgcaagtt cttgattcta tggatattga acgtgagcga ggtattacca tcaaagcgca
    22801 atgtgtttcc ttgaattaca cagctaaaga tggaaagacc tatttattga attttatcga
    22861 tacgccaggg catgttgatt ttagttacga ggtatctcgt tctcttgccg cttgtgaagg
    22921 tgctattctg gttgttgatg cagcacaggg agttgaagcg caaactttag cagtttgtta
    22981 tacagcaatc gatcagtctc ttaccgtatt gccagtactg aataaaatcg atttgccgca
    23041 ggcagaacct gagcgagtta tttccgagat cgaagatatt attggtctcg atgctcaaga
    23101 tgcgattaga gtgagtgcta aaagtggctt gggtgttaat gatgttttag aggcattagt
    23161 tgcgaatatt ccccctccga aaggcgatgt tcatgcccca ttacaagcac ttattattga
    23221 ctcctggttt gatagttatt taggagttgt ctcactggtt cgtattgtta atggcgccat
    23281 acgaaaaggg gataaaatgc gagtgatgtc cacaggcagg gcttatgaag tggatcaagt
    23341 tggtattttt acccccaaac ggactaaact ggatgcttta tatgctggtg aagtgggtta
    23401 tgttgttgct ggtataaagg aaattcaggg cgctccagtt ggtgatactt taactctgga
    23461 tagaaatcca gctgataaag ttcttccagg gtttcaacgc gttaagcccc aagtttatgc
    23521 aggtttgttt ccagtcagct cggatgattt tgaggcattt agagaggctt tggctaaatt
    23581 gagtttaaat gatgcttctt tattttatga gccggaatct tcagaggctt tgggttttgg
    23641 ttttcgttgc ggatttttag gcatgctgca tatggaaatc attcaggaga gattggaaag
    23701 agaatataac cttgatttga tttctacggc acctactgtt gtttatcaga ttgtaactca
    23761 gaaaggggaa actttattaa ttgataatcc ttcgcatttg cccccaaccc cgcaaataaa
    23821 agagatgtat gagccaattg tcagagcaaa tattctagtg ccacaagatt atttggggcc
    23881 tatcattacc ttatgtgttg aaagacgtgg tgttcaagtc agtatgacct atagtggtcg
    23941 tcatgtttct gttgtttatg atattcccat gagcgaagta gtctctgatt tctttgatag
    24001 attaaaatca gttagtagag gttatgcctc tttggattat aacttccaaa gattccagat
    24061 tgctgatttg gtcaagatgg atatattaat aaatagcgaa cgtgttgatg ctcttgctgt
    24121 gattgttcat agagattccg ctcatagccg cggaaaatta attgcggaga agatgcaaca
    24181 gcttattcca agacagatgt ttgatgtggc tattcaggct gctataggaa gccatattat
    24241 cgctaggcaa acagtgaaag cattgcgcaa aaacgttaca gcaaagtgtt atggtggtga
    24301 tgtgacgcgt aagcggaagt tgctagaaaa acaaaaagct ggcaagaaaa gaatgaaaca
    24361 ggtggggcat gtagaaatac ctcaagaagc atttatggcc gtgtttcaaa cagataaaaa
    24421 aaagtagagc cgctcattta ctaataagaa tgtattgacc ttttcgtcag catctggaca
    24481 taagtcaaat catgattaaa ttgtcttttg cttaaaattg aggatattga atttatgaat
    24541 tttgctttaa tattggtcat attatccgct gtaacaggat tgattgcttt attggattgg
    24601 ttgatttggt ctaaaaaacg tttgcctgaa caaaagccag gtcgaattat tgagtattca
    24661 cgttcttttt ttcctgtatt ttttattgtt ctgctattgc gatctttttt agttgaacct
    24721 tttcgtattc catcgggctc actcgagcct actttgttgg ttggtgattt tgtagcggtt
    24781 aataaatttt cttatggact gagacttcca gtatgggaga aaaaaatagt gccaattgct
    24841 aatcccaaaa cgggtgaaat tgcagtattt cgctggccac ctgaaccatc ttatgactat
    24901 attaaaagag ttattggcgt gcctggggat aaaatttcat atcatgataa aaaactaact
    24961 attaatggca aagaggtgaa acagaccttt gttgagtata ctaccgatga aagctctggc
    25021 aaggctgtga caaagtataa agaagattta aacggtgtgg tgcacgacat atttgtcaga
    25081 cctgatgcgc cagccgtaga ttttgatata gttgttcctg aggggaatta ctttatgatg
    25141 ggagataatc gggatgatag cgccgatagt cggttctggg gatttgtacc tgatagctat
    25201 ttaagaggaa aagcatttct ggtttggatg agttggaatg gcaaaacaga taatgtacgc
    25261 tggtcaagga taggcaaatt aattcattga gggaataggg catgagaaaa caacagggaa
    25321 tgacattgat tggtatgctg tttactgccg ttgtggtgat aatggctggt atacttgtta
    25381 tgcgggcagc gccagtttat attcaacatt attcgatttt gcattcaatc aaagcattaa
    25441 attcaacacc tgtttcttct ttgtcggggg atcccttgac cgatgttaca actttaaggg
    25501 aaagtgtgac taaacgatta gatatcaatg gggtagagga cttaaaacca ggagagttgg
    25561 ttattactcc ttcaggcccc aataaattta aatttaagtt aaaatatcaa gtcatcaaac
    25621 ctctaattta taatatgagc ttattattta attttgaaga taattttgag gtaataacag
    25681 gtagtgaaaa ttaatttaga aagattgtgc agacgtttaa actatcattt taataacata
    25741 gcctatttga agcaggcttt gacgcattgc agtgctggta gcgataatta tgaacgcttt
    25801 gagtttcttg gagactcgat attaagtttt gttattgcca atgagctatt caatcgtttt
    25861 ccacttcaca gcgaagggca attaagtcga ttgcgctctt ttcttgttaa aggtgaaatg
    25921 ttagcagaaa tagccagaga aataggtttg ggcgattatc tatttcttgg gcaaggtgag
    25981 ttaaggagcg gtggatttcg ccgcacatct atattagcag atgcccttga ggctatattg
    26041 gcagccattt atttggatgg aggaatgact gctgcaaaac agattatttt aatgctttat
    26101 agttcacgat tggatgatcc ggatttaaat cattgtttaa aggatgcaaa gacacaatta
    26161 caggagtttt tgcaagcatc aaaattcgct ttacctgaat acgtcctaac caagattgag
    26221 ggggatgagc acgctcaaat cttccatgta acatgcacaa ttgagggagt aagtcaggta
    26281 gcttatggca cagggcctaa tagaagaaaa gctgagcagt tggcagcaaa agccatgttg
    26341 gaacagttac aaggttaaat ggtattgatc agcagaacct cttacttact ccaggctttc
    26401 cagggcatga catatttcta accactcttc ttctgtctta attaaagcgg aatgagcgat
    26461 atctcgttcc atcaaaagat tatccaattt gcttttctgc tttgtgtctt catacagaga
    26521 aacgtcagct aactgcttat caagaaggcg caattgattc tgatagtgct caatgagttg
    26581 ctccagcttt tttaatttgt tttgatagct ttttctttct ttatattcat ttggggaagt
    26641 agctaatttg gattcctttt ctgaatttct ggtttgcagc cagagatagt aatcatcaag
    26701 atccccttca aatggttgta cttttccttg ataaataaga taaaaattgt ccacacttga
    26761 ttttaacaaa tggcggtcat gcgaaatcaa aattaaggcg ccctcataag tttgcagcgc
    26821 taattcaatt gctgagcgca ttccaagatc caaatggttt gttggctcat ccagcaaaag
    26881 aaggttgggt tttagccaaa ccagtcttgc taatgccagg cgggcttttt caccacctga
    26941 aaagtgctga atggattgta ccgccatatc ccctctgaaa ttaaatccac ctaaaaaatc
    27001 ccgtatggtt tgttctctta cctctggcga gagagtttgc agtgtttcta ccgggcttaa
    27061 tttacagtct aattgctcaa gttgatgctg agcatagtag cctatttgta aatgcgggga
    27121 acgatggata tctccactta atacgggcaa atctgccgta agcgttttaa ttaaggttga
    27181 ttttccttca ccgttaggtc ccagtagtgc aattctatcg ccgggattaa gagagagttt
    27241 gatttttttc aaaatcgggg gatgattgat atatcctgca tcaacttgat tgcattgaat
    27301 taaaggattt cctgcccgag gacaagggaa aaaatcaaaa gaaaaagggg agtcggcttg
    27361 ggctgctgcg attatttcca tcttttctat agctttcatt cggctttgcg cttgtttggc
    27421 cttggttgct ttcgctttaa aacgagttac aaaagtcatc atatgtttga ttttggtttg
    27481 ttgcttttca tgcattgctt gttgtagagc caattgttgc gcatgaattt tttcaaagct
    27541 gctgtagttt cctgaataaa gtgtcagact acgattttct atatgtaata tatgagtaac
    27601 aaaggcatca agaaattctc tatcgtgtga aataaggata atagtacttg ggctttgttt
    27661 taaatagcgt tccagccaga agatggcttc catgtctaaa tggtttgtgg gttcatcaag
    27721 caggagtaaa tcggcgggtt tcattaagca ccgagcaaga tttaagcgca ttctccagcc
    27781 accagaaaag ctattaacgg gctcttgctg ttgattgcca ctaaatccta agccagacat
    27841 aataactgct gcttgcgcgg gcttactgta accaccagta tgagagagtt cctcgtgaca
    27901 ttgcaaaact tctgcatcat cgccgttttt ttcagcttct gaaaggcgct ttagcaattt
    27961 aatgtattgt tgatctccgg caagaacaaa atccaaggct ttttcattgc tatcaggtat
    28021 gtgttgagac aaatgactga tgctgagttg tggattaatt agatattctc cagtatcagg
    28081 agataattga cctaaaagaa aatcaaataa tgttgatttt ccacatccat tatgaccgat
    28141 aaggccaatt ttttgttttt cataaagact gacattgacc ttatcgagta aaattttatc
    28201 tcctctggat aaagtaattt gacgcaatgt aagcatgtta gaccgtaagc ttgaactaat
    28261 cagccatttt atcataaatt atcttaagct tcctattgat catgtaagca agttggatat
    28321 ttgcataagt tcaaaaccgg attttcttca aaaataaatt attcgctggg ctgaattatt
    28381 ttctttcgtg ccgattgata acgtgcttgc ttttctatat caatatacct gtccgttgta
    28441 gcgcttgaac tgtgcccggc atcatctcgc acatgctctc tgggtctgat ttttacatct
    28501 tctgatatac cagtatgccg cagccaatgc accgtggcag cggatagatt atctgcttcc
    28561 tctgataaac cattaagtcg cagttgttct tctgccaagt caaaacaatg ctgaacaatt
    28621 ctccttatag gagcagtgtt actcattgga ccatgaccac gcagcttagg tagtaaaggc
    28681 gaattatcag ctggtgatgg cagtggggta agtccaagaa ataatcgcca gcgggtaagg
    28741 ctttccagca tagcgtcgct taccgcaatt tgtcgttcct tatttccttt tcctacggtg
    28801 gtaaaccacc agtggccatt actgtccttg gcaaaatcat tcatactggg tacccatctc
    28861 tcactggcag caagttctga tatacgaaga tacatgccaa ataacagact taaaataaaa
    28921 cgagtgcgtt catgtttttc aggggaatct tcagccaaac tttcagctgc tctcaggaca
    28981 gcttcccatt gaaccagact taatcgtcta acaggcgcgt tatgctgtct tttgcgaata
    29041 aatttgcttt tttgcctaat aagggcaact ggattggata tcagatattc ttcagcaatt
    29101 agaaagttga atagagtact taagatggcg aatatttctt gcacggctcc ctgagataac
    29161 ttgaattgat ctattgatgc aagatgccca tttttcctgg aaaccttact tagtgtcact
    29221 acaaaaggtc gccactcttc attaggtatc cgtttaccat ttttttctat aaatcgtgga
    29281 acttttttta gactaatcca tgttttaggc gggttttgac aaaaatggat gtattgttca
    29341 atatcgtcac gccttaaaga atcaatagta gagtttttga caagccatga ccactgtaac
    29401 aaacgttctg tttctcttct gtaactgttg aatgtgcctt cgcttcctat atagcttttt
    29461 aaaaaactta agcaataagt gaaatcattc agaaatcgca catcaggtaa tatcacattt
    29521 tcatgatttt tatggatgtg attgagatta tcaaacaagg gtaatagttt tgttttcatg
    29581 atcataactt atctgctgat atgtcattat gctaacctaa ttgctgattt aagtcatagg
    29641 gtacatgcgt tattgtaaat aatagtgttg agccagctaa gtaagtgcct gtaaagggca
    29701 ataaattgac taaaagtacg gaagaaattc agattagaat tcagactcta taataaataa
    29761 aaattatatg ctaaaggata ttgaccttca tgatagcacc tagtattatc tgcgctctgt
    29821 ccttgggacg ggtatcgggg tgtagcgcag tctggtagcg cgcctgcttt gggagcagga
    29881 tgtcgggggt tcaaatccct cctccccgac catttaagcg cccgtagctc atctggatag
    29941 agcatcggcc ttctaagccg agggtagcag gttcgaatcc tgccgggcgc gccagccgcc
    30001 ttgcttttgc agtattatcg ctggaacagg gcttacgaaa ccctccatgg ttgcataatt
    30061 tttaataaaa acctattttg tgcaagtagc aatattgaaa atttatgaac gtataatttg
    30121 ggaagtttcg taagccctgt taagagtaat tttggtgagc gtagctcagt tggtagagcc
    30181 ccgggttgtg atcccggttg tcgtgggttc gagtcccatc gttcacccca tttgtaacag
    30241 atataatttt gagtctaccg ctaaagattc catctgagtc tttacaagac tgaattactg
    30301 attgataatc caacctgatt gcgaaagtgg cggaattggt agacgcactg gatttaggtt
    30361 ccagcggggc gacccgtgag agttcgagtc tctcctttcg caccatttca tgataacttc
    30421 cgagaggtga attaatgcaa gtttctgttg agaccctaga aggtttggag cgtaaggtta
    30481 cagtttctgt acctacagag aaagttgagg aagaggtgag ctcgcgtctc agaaatcttg
    30541 ctcgcaaagt gaaaatagat ggttttcgtc caggcaaagt cccttttaat gttgtaaaaa
    30601 gccgtttctc tgatagtgtg cgtgaagaag ttgcccggga aatggtccaa tctaccttat
    30661 atgaagcctt acaaaaaaat gaattggttc cagcaggtta tcctcatgtt gagcctttag
    30721 aaatcgaacc aggtaaggat tttaaataca ctgctgtatt tgaagttatg cccgtttttg
    30781 aaattgtcga actcaatcaa gctccagtgg aattaattcg atctgaagtc actgataaag
    30841 atgttgacaa catgattgag aaattacgag aacaaaacaa agagtggcat gaagtaactc
    30901 atgcggtcaa aaaaggtgat aaagttgtta ttgattttca gggatttttg gatgataagc
    30961 cattccaggg cggcagtgct gaaggacatg agcttgtaat tggttctggt tcgatgattc
    31021 ccggatttga agatgggatt gttggtggga aaattgacaa accgtttgat ataaaagtga
    31081 gtttcccgga agattatggc cataaagatt tggctgggaa ggaagcaacg ttcaagatca
    31141 ctatcaaaaa gattatggaa ggcaagttgc ctgctttgga tgaggcattt gcggagaaat
    31201 ttaatataaa agaaggtggt atagagtctc ttaaaaagga tattagagaa aatatggcgc
    31261 gtgaattgga acgtcgcgtc aacatgatga acagagagaa attgtttgat tcattaatgt
    31321 ctgtgaatca tgttgagtta ccaatagctt tgatagataa agaaattgag catttaaagc
    31381 atgatatgta tcatcgattg tttggtcacg aacataaaga cgatgaaaaa attcctgatt
    31441 tcccaaggga gctatttgaa gaacaagcca agcgacgtgt acacctgggc ttactttttg
    31501 ctgagtatgt aaaaaaacac gaaattgttg cagacaacga taaagttaat gctatgattg
    31561 ataaatttgc cagtgcttac gaaagtcctg atgagcttcg cgcatggtat caaagcagca
    31621 aagaacatat ggctgaggtt gaagcattag ttatggaaga tatggttgct gataaaatag
    31681 cagaagatgc gaaacttaaa tataaaaata tggattatga ttcagtgatg aatcccaaaa
    31741 agggcactga gaaaaaagga gagtaagcat gccaggctat tcagataata ttatccgtaa
    31801 tgcaagcgga ttaataccaa tggtaattga gcaaacctcg cgaggtgagc gttcctatga
    31861 tatttactca agattattaa aagaaagaat tatttttctc ttgggtgaag tagaagatca
    31921 tatggctaac ctggttgtgg ctcagttatt atttctggag tctgaaaatc cagagaaaga
    31981 tatatctcta tatattaatt ctcccggtgg agttgttact gcaggtcttg ctatctacga
    32041 caccatgcaa tttattaagc cagatgtaag tacattatgc ataggccagg cagcaagtgc
    32101 agctgccttg ctactttgtg ctggtgctga aggcaaacga ttctgcttac ctaactcaag
    32161 agttatgata catcagccct tgggtggcta tagaggccaa gcaacagaca tagaaattca
    32221 tgctcgagag accttggcag ttagggaacg tttgaataat attatggcta aacacactaa
    32281 aaaaacacct gatcaaatca tgcgtgatac agaaagagat aactttatga gcgctactca
    32341 ggctatggag tatggtctaa ttgataaagt tctctatgac agacaagttg ctgggcattc
    32401 tacagactta taactttgct agatactcct agtttatctg agtataatat aagttggttt
    32461 gatagtaatg tggtggcgca atattatctg attgtctaaa attttacatg taattactat
    32521 taattagtaa tagctgtaag aaggggtttg gttatgagta aatccggtaa cggaaatgga
    32581 gataaggtct tatattgctc tttttgtggc aagagtcaac atgaggtaaa aaaattaatt
    32641 gctggacctt ctgtgtttgt ttgtgatgaa tgtgtagagc tatgtaatga tattattcga
    32701 gaagaaacac atgaaactca tgaagaaact gaggcacgtc taccgacacc aaaagaaata
    32761 tctaactttc tcgatgaata cgtaataggg caacaacatg ccaaaaaggt tttatcagta
    32821 gcagtatata atcactataa gagattacag cataaaagcg aggatggtgt tgaactcggt
    32881 aaaagtaata tactacttat aggacctact ggcagtggta agactctttt agctcaaacc
    32941 ctggcgcgga ttcttaatgt tccttttgcc atggctgatg ccacgaccct tactgaagct
    33001 ggatatgttg gtgaagacgt tgaaaatatc atccagaagt tattacagaa gtgtgactat
    33061 gacgtagata aggcacaaca aggcattgtc tatatcgacg aaatcgataa gatttcacgc
    33121 aaatcagata atccatctat caccagagat gtttcaggag aaggtgttca acaagcttta
    33181 ctgaaattaa ttgaaggtac tgttgcatct gttccaccgc aaggtgggcg aaagcatcca
    33241 cagcaggaat ttttgcaagt agatacatca aatattttat ttatctgtgg tggcgctttt
    33301 gcaggtttgg acaaagtaat acgtgaacgc agcgataaat caagcatagg tttttctgct
    33361 caattaaaaa gcaaaaaaag cagtaatgac gaagcttcaa aagttttagg tcaattggaa
    33421 tcagatgatt taattaaata tgggttaatt cccgagtttg ttggacgttt gcctgttgta
    33481 accactttac aggaacttga tgaagcggca ttgattgata tattaacgag accgaagaat
    33541 gctttaacaa agcaatttca atcacttttc aaaatggaag gttctgaact tgagtttaga
    33601 gacgaagctt tgattgctat tgctaaaaag gccctcgaaa gaaaaatggg cgcaagaggt
    33661 ttgcgatcta tacttgaaaa tattctttta gacaccatgt atgacttacc ttccttggaa
    33721 ggcgtgaata agattgttat tgatgagagt gtagtgaatg gactatctaa accgattctc
    33781 atttatgaac aggatgagaa aaaaagtgcg tctggtagta aagattaagt aagatgttga
    33841 tttctgcaat aatttaaatt gttgcagaaa tcattttata tttcattcac aaaatagatt
    33901 taaagctgat tttgtagctt aaaataacaa atttagtcat cttaatagca atagactaac
    33961 tctcacatta cgaattgttc accagcacca atatctgtat actcataagt aatagtataa
    34021 gaaactgtaa ccaagaaatt ttcctttgaa taagggtcat tatgtctaat gaaaatgaaa
    34081 taatatcaaa tgagactgtg aaatcttcag ctttgcctgt attaccattg agggacgttg
    34141 tcgtctatcc gcatatggtg atacctctct ttgttgggcg tggcaagtca attaaagctc
    34201 ttgaagcagc gatgattgat aataaacaaa tttttttagt tgcgcagcgt aaatcggcgc
    34261 atgatgatcc cggcccagaa gatatctatc aagtgggaac tgtatccagt gttttgcaat
    34321 tgttaaagtt acctgacgga actgtaaaag ttcttgtcga aggtgagcag cgtgcgcgtg
    34381 tcaaggagta tactcaggat aaaggttacc tcgaagcgac acttgaatac attgaggaag
    34441 ttggctcaac aattcaagag caagaaattg gaattctaat gcggtccttg atgtcgcaat
    34501 ttgaacaata tataaagctt aataagaaaa ttccaccaga agtcttgtct ccattggcag
    34561 gaatagaaga gccaggtcgc ttggccgata ccattgccgc acatttaacg cttaaagtag
    34621 atgataagca agagttgctt gaaactatgg atgtaggaac tcgacttgag aaattaatgg
    34681 cggcgataga aaatgaaatt gatctgttac atgtggaaaa acgtgtaaga ggtcgagtca
    34741 aacgtcaaat ggaaaaaagc cagagagagt actatctaaa cgaacaaatc aaagctattc
    34801 agaaagaatt aggggaaatg ggtgaggaag gcaatgagat tgaacaattg gaaaattcaa
    34861 tcaaaaaggc cggaatgccg aaagaagcta aagagaaatc attagcagag ttacataaat
    34921 taaaaatgat gtctccgatg tctgctgaag caactgttat tcgtaattat cttgattgga
    34981 tgctgatggt cccatggaag aagcgtacca aaattcagtt tgatttacta aaagcagaaa
    35041 agctgctcga taaggagcat catggtctgg agcaggttaa agagcgtatc atcgaatatc
    35101 ttgctgtaca acaacgagta aaaagattga aaggaccgat attgtgtcta gtaggtcctc
    35161 caggagtagg taaaacttct cttggacaat ctatcgccaa tgctactgga agaactttca
    35221 tacgcatcgc tctgggtggg gtaagagatg aagcagaaat tcgtggtcat cgaagaacat
    35281 atataggttc tatgccaggt aaaattatcc aaaaattatg caaggcagga gttaaaaatc
    35341 ctcttatcat gctggatgaa gtagataaga tggcaatgga ttttcgcggt gatccggcat
    35401 cggcattact ggaagtattg gatcctgaac agaaccatac atttagtgat cattacttgg
    35461 aagttgatta tgacttgagt gatgttatgt ttattgcaac tgccaattcg ctagaaattc
    35521 cagccccgtt attggataga atggaagtta ttcgcttgcc tggatatact gaggacgaaa
    35581 aagtcagtat tgctgaaaaa tatttggttc ccaaacaaat gctgttaaat ggtttaacca
    35641 atgaagaaat ccatattagc gaaggggcta ttagagatat catccgctat tatacgcgag
    35701 aggctggagt acgtaatctt gaaagggata tagcgagtat atgcagaaaa gttgttaaag
    35761 aaatactctc aagcaaaaag ggaaaaaaag gaaaaacaat cagtataact gttagcaaca
    35821 ttactaagta tttgggcgtt aaaaaatatc gttatggatt agctgaacaa tacgatcaga
    35881 ttggtcaagt cactggctta gcctggacaa gtgttggagg tgaacttctg actatagagg
    35941 catccattat gccaggtaag ggtaaagtta ctcatactgg tcagctcggt gaggtaatgc
    36001 aagaatctat ccatgcagct atgacggtag taagaagccg cgctaaagct tttggtttac
    36061 cagatgattt ttatgagaaa catgattttc atgttcatgt accagaaggt gcaacaccga
    36121 aagatggccc aagcgctggt ataggaatgt gcacggtact agtctccata gtaacacaaa
    36181 tcccagttaa ggcagatgtc gctatgacgg gtgaaattac tctgcgtggg caagttttac
    36241 caattggagg attaaaagaa aaattgctgg ctgcacatcg tggtggaatc aagcatgtca
    36301 ttatccccga ggagaatgta aaagaccttg aggaaatccc tgataatgtg ctccgcaagc
    36361 tgacaattca tcctgttaac accattgaac aggttttgga actggctttg caacgcaatc
    36421 catgggtgga agagccaaca aatatacaac ctggcactat attgaataaa agatcaaaaa
    36481 aaattaaaaa taatgattta cacgcacatt aatacagagt atatttctct acgtggcccg
    36541 ctactggcgg gctctgcaag gatgtacgaa cttgactgga gcagttgaat ctgtttaaat
    36601 cagctccgga atatggatga ttaactgtaa aggggaaaag atgaataaga gtgaattagt
    36661 tgatgctata gcaagcggtt caggtttgac caaagctgac gcaagcagag ttcttgaaac
    36721 ttttatggct actgtaactg atgtcttaaa gaaaggcgat caagttgtaa tacctggttt
    36781 tggttctttt tcaacaggta atcgttcagc acgtactggc agaaacccac agactggaaa
    36841 aacaatccaa attaaagcgt cacgcgttgc caaatttaaa gcaggtaaaa acctgaaaga
    36901 agcggtacaa caagcttaag ttttcagggt gcttagctca gctgggagag catcgccctt
    36961 acaaggcgag ggtcgtaggt tcgatcccta cagcacccac caagaaatgg agtggtagtt
    37021 cagttggtca gaataccggc ctgtcacgcc gggggtcgcg ggttcgagcc ccgtccactc
    37081 cgccagataa acgcgctgaa aggcgcgttt tttttatcag cttatggtag aatcgttttc
    37141 atcaagcatt gttaatatca aaatggatct cgggacatgt tgcagaagtt aaatgaacac
    37201 attcagggtg tagtagcgtg gttagttatt atattaattg ctattacctt tacacttttt
    37261 ggtgttgatt attattttca atctcgacaa atatccgatg caaaggtaat agttaatgat
    37321 aaaccaatta ccatgcaagc gtttgaaacg aattacagac gtactcgtgc tcagcaagat
    37381 ttaccacaga tgactgccgc tgatgaaaaa aatctgcaaa atcaagtatt aaatcaaatg
    37441 ataacgaatg aggtgagtat tcaggctgcc aggaaatatg gatttgaggt tagtccagaa
    37501 caagccaatg cggccatagt gcaaattcct caattccagg aggatgggca tttttcggca
    37561 caacgatatc aacaagcttt gagtggagct ttgtttactc cagagacttt ccagaatgaa
    37621 gtaaggcaag gcatgttgct taatcaacaa cgttttgcgt ttatgggaac atcatttgct
    37681 ttatcagatg aaataaagcg ttttgtcaga ttgtacatgc agaccaggga ttatgattat
    37741 ttgacggtac cttctgatcg ctttgaacaa caggcgaaga tttcagaaga tgatatcaag
    37801 agttattata accaacacca taaaaaattt atgacttcag agcaagttac attggattat
    37861 gtgcttttgt ctatgcatga tatcaagtca caaataaaga tatcacctga ggaaattaaa
    37921 aattattatg aagaaaacaa aaataactac ctaactcctg ctcaatggca ggttgcccat
    37981 attttatttg cagttccaga aaatgccacc aaggaagaac aagattcaat taagcaaaaa
    38041 gccgatgagg tttatagcga tttaaaaaag catccagagc aatttgataa atatgttgct
    38101 tccaaatctg atgacaagtt atctatagcc aataagggta ttcttccctg gattactggc
    38161 gggcaaaatg aatatggcag agtactttcc aatttaacta gaccagggca aatttctata
    38221 ccagtacaaa caaaatatgg ttatgaaatt tttaagctga ttgcttacaa gccagttaca
    38281 actaaatctt tgcaagaagt tgaaaacatt ataaaggatc aactaaccag tgatatggct
    38341 caggcaaaat atgctcaagt tttagagcaa ttaacagatt taagttatca aactcctgat
    38401 tcacttgatc cggttttgga ttcattacag ctgaaaattc aacatacaga gcctttttct
    38461 cgtgatggtg gcaagcaacc tattaccaaa aataagcagg ttatccatgc agcattcagc
    38521 actgatgttt tagaactagg gaataatagc gagcctatac agattgataa tgactctgta
    38581 atagttttac gagtgaagaa acatataccc tcaagagaac aaaccttgga tgaagtgcga
    38641 aatcagatta aagaaatact tgcaaaacaa tatggggatg ctaaagcaaa agaaattggt
    38701 agtagtttgc ttcaccccgt tgaagataag caacagcagg aattaatcgc taacaatcaa
    38761 ttcgaatggc attctgtaga gaatgcttcc agggatagtg ataaagcaaa ctctttaatt
    38821 aatgatttag catttaattt attacgtcca gaaagccgag atggagtgat tttagacaac
    38881 ggcgactatg ttgttgttaa attaaaacga attaatgatg gaaaattaag ctctctggat
    38941 caagagcaaa gagatagctt gatacagcaa atagaagcta gctatggaat gatggattat
    39001 gatttatatg ttaataactt gcttcatcat gcgaacatag taaggaaata ggataagaaa
    39061 atggctgttc cttacgaaat taagggatgg ccagatatcg agaattcttc aatttttgcc
    39121 tatagtttct ctctgtgagg ctttttcaaa gttgctcctg ataaaaatat cacccatatt
    39181 attaataagc ctgaaactgt tcatagactt acgcgttgaa atattttata gcggttggtt
    39241 tggggttcag gctggacgaa aatgatcctg aatagagcgc aggtggttac atgagctttt
    39301 gaaggattaa tttcattcaa aatcagctaa atatggtctt tttaggttga ggtttattct
    39361 cttaataata atccgttaat gaaggagtcg caggagtcat ttgattgagg tgctctattt
    39421 catgtcgatt caatagatta gataggctaa atctaaattt gtctgttata ttataaaaaa
    39481 cgtttggtag cttgggcctc gctatttgct cagcccaaat ataatttagc tttattgaat
    39541 ttttttaagt acaactaaga attaagtcag caatttaaag tggactaata tttatctagc
    39601 ccaattcact cattgcggat actgcatgat atcctgcatc aacatgtagc acttcaccag
    39661 taatccctga tgccagatca gagcataaga aggcagcagt atttcctact tcatcagcag
    39721 taacatttct ttgtaatgga gtaatgtttg cataggcagc atgaatttta cgaaaatctt
    39781 taattccagc agcggcaaga gttttaattg gtccagctga tatggcatta attcttaatc
    39841 ctctggatcc taagctggct gccagatagc gcacagaggc ttccaagctt gccttcgcaa
    39901 cacccataac gttatagttt ggtacggctt tttctgcccc ataataactt aaggttaaaa
    39961 ttgaaccctg agtatcaagc atcataggca atgcggcttg agacaaacct atgaggctat
    40021 aggcactaat atcatgagct attctaaatc cttctctatt tgcgcattcc acaaaatcac
    40081 cactgatctg atcagcagga gcataagcta cagagtggac caggatatct aatttatccc
    40141 agtgattacg caaattatca aacacggctt ttatttcttc atcacttgca acatcacaag
    40201 gaaaaactag agttgaatta aattccgatg ccattgcttc aacacgcgat tgtaattttt
    40261 cgttttggta agtaaaagcc aactcagcgc cttgattatg gaatgcttta gcaatgccat
    40321 atgcaatcga tctgttgctt gccaatccaa caataagtgc tttttttcca gttaaaaatc
    40381 ccacaatagt atctcctccc attattctta atttagcagg gcgaagtgag tgttacttcg
    40441 atcctggact aaaaccacaa tatcaattag gttgctctta aacagcctaa taaaacatca
    40501 actgcctcga gtatgtgaag ctaataattc ccgcattttg gcttgaatca tctctatagc
    40561 gattctgttt tcaccaccgc gaggtactat tatatcagcg tagcggcttg aaggctcaat
    40621 aaattgcatg tacatagggc gaactgtagt ctcatattga tgaataaccg attcaaatga
    40681 tctatgcctt tctactacat ctctttttaa gcgccgtgtt aaacagacgt caaggggagt
    40741 cgacatgaat atacgtatat ccatgatttc gcgtagagcc ttatcactga aaagcagtat
    40801 tccttcgagt acaataatgg catgttgacc cactgctctg gtttcaggta aacgcagatg
    40861 tttggaatga gaataaatag gaatttgtac tgtatttcca actcgtagct gacgaagatg
    40921 ctcataaagt agagcatgat caaaggagtc aggatgatcg taatttattt tttctctctc
    40981 tgcaaagggt agatgcccat tatctttata gtaggcatct tcagagataa cgacaacctg
    41041 ctcggatcct aattcattga ctatagtatt tgctaataaa ctttttccag atgctgatgg
    41101 gccggaaatc ccaataataa ttgcttgctt tgtcataatg ttttcattaa aaattttgta
    41161 cgaatagtaa agttttttcg gttggaattc aatcattctc tccagtctta tgaaaagcat
    41221 tgactacttc aaccaaaggc tgtcccactg ctgcggcagg gggtttaatc aataataagg
    41281 aagataatgt tggtgcaata tccgttgtgt gtacttgcct gaatatacgt tttggtttga
    41341 ggtcagggtg tacaaacagt agcgggacat aagtatcata ttgccaggga ctgccatgag
    41401 caactcgatc ttcacttttt gcaccgtggc tttgatatgg cggttgaaca atgtaaacat
    41461 cgcctgcacg gtaagcgtaa gacatgcggt caacttttgc gcttaaccaa tctttttcaa
    41521 tatcacgaat aggtaagggg taggctttga atatccctgg aagatgactg atttcttctg
    41581 ctaggtagaa actgacctct gcaatatcca aatttttctc ttttataatt tggtgatcaa
    41641 ggtatataaa aggaggatta attgacatta aagcttgcgc aggcaggttg aaacgtttca
    41701 ataataaatt ctcgatgtgt tttcctgttg cttctatatt aacaggttta atttcaggta
    41761 ttttatgtgc tttaaggtaa gaaggaccat cgctgacacc atgatctgcg gtgagtataa
    41821 ttaaggtatt attaagacca acctctttat caatggtttt aaaaagttgg cttagggtgt
    41881 catcaagggc gatcaaattg tcttctgctt ccagactatt aggcccaaat tggtgaccaa
    41941 tagcatctac agcagaaaaa ctgattgcaa gataatctgt ttggcctgtt gtttttccca
    42001 atttttcatt aaccagcaat tgtttggcaa aatcagctgt taattcatct gcctttggtg
    42061 ttctggaaag cgatttgaaa tattcttcag atggaggatt ggttacatga tggggaaata
    42121 tttgaccaaa gctgccatag ttatgacgaa atggaagagt atttgcattc tgatagaatg
    42181 atttaggatg agaaagattc cagtcgtagg ctttgggttg atagtgttta ttccagtttt
    42241 ctacccatag tggataactg gaatagtagt aattgcttgt aataaatcca ccattcgttt
    42301 tgtcaaacca aaacgctttt cctgcgtgac cagctagagt gatagctgcc ctgtctttta
    42361 atgatacacc aaaggctttt cctttatttg ctaaaatgat ttcatcgctt aatgtggatg
    42421 gttttaggtt tcgcggtgag cgtcctgaaa tttctatatg ggtgtgtaga gtgggtaaaa
    42481 ttttagcttg taaatcctcc atacagtact ttagttttct tgtttttcta tcataccaat
    42541 cattatcaac tatgccatgt aaagaggggt agcttcctgt tgcaatagtg ctatggcctg
    42601 cacaggtcgt tgtattggca tgtggatgat gtgcgttatg ataatccaaa ccatgagcaa
    42661 gcaaataatt gaaaccagaa ggtccaaact gtttatgatg acggtaaatc agatctccgc
    42721 gtaattggtc aacaactatt tgtaaaatta atttaggttg acctgaatcg gcatagctaa
    42781 ttagggagtt gatcaaaaga agaatactgc agcttatttt tttcattttt atgaccatca
    42841 tatattgcaa attgacgcgt aaattaaatc aaatgtttta taaaaacaat aaatagctta
    42901 tttaaatttc ttctaatttt tattgtgtct gactgattga gcaaaaaata tcttgattta
    42961 attgatctga gcttactctt aaagcgacga taacaaggct taaaaatact ctccagaagt
    43021 ctatttattt gatgaactaa caaggaaatt gcaaattttt cattaaaatc aatagattga
    43081 aaattaatta gatagtaaaa agaaaaagca tgacgaaaag agagaattgg tttaaactgg
    43141 cctacagtga aagcatttca ctagtcctgt tcatggagta taaggagaaa agaagatgtc
    43201 atttgaattg gtcgcatatg aaaaattaaa aggaagcatc agggaaagta tcattacttt
    43261 aatcaagtcc cataatgaga aagctaaaat tattgaggac aaattagagg actcagtaaa
    43321 agaagtttca agagaaaggc aaccacaagt acttgtcttg ctaaaaacta tcgaactgct
    43381 ggataactca tccaaagaac cagaagataa ggcacgcgta ttaaatgcat tggcctatta
    43441 cattcgtgat cagattgctg caacttataa atacacctct cccgacaaca gtgattttta
    43501 taaatctctt accatatcac ttgatttgaa taaggataat aatcctaatc gggaagattt
    43561 ggctgatatg tacagtgctt tggaaaaatt ccttcgttcc catgtttata aaaattctga
    43621 cccaagaaaa gggtatttgg ataaacaacc tttcgcaatc aagcattatt ccgttgttga
    43681 tgatattctt gaattatctg acagagtgca taaattaaga cgtgaaatta ttatcgccgc
    43741 ccgagatttg catttactgc aacaaaaacc aaagagctct caaggcggtc tttttagtgt
    43801 tccatcagga aaagaaaaag tcaccgatac taatgaggtt gtaaatcatc gtctttaagt
    43861 aactcattat cttttattct cacctcttca ctcaggaagg ggtgtaagac tcctgtttta
    43921 ccgggtaacc actcattgcc caagccataa ttccaccatc cacagaatag acttcgcaat
    43981 atcccaaatc cattaagcat tgagccgcat acagtgatct gacgccactt cggcaatgta
    44041 aatagatagt ttgctcttta ttgggtattt gagcttgtat ttcaatagag atgcgatctt
    44101 tagggatatg aagtgctccc gggatatgca tcatttccca ttcgtccagt tcacgtacat
    44161 caatcaaact caaattggct tgattgtcca tttggtgttt taattcatga acgtctattg
    44221 ttttgatctt atgctttgtc ataacgaatt tctctcatag tgggtctggt taatgtggag
    44281 tgatgaagca gccaatatat agttgccgtg atgactcctc caattaaagt cgcaaatgcc
    44341 caggtagggg ccgatactaa aacaggtttt tggcctagcc tatagagatt acctgcgtct
    44401 cgagccactg ctccggcaaa gataaaatgt aaaagaccat taattaacat aattaaataa
    44461 aaaaaggttt ggatttgtgc gctaaattgt tttgctaatt cactgagcat gaatcagatt
    44521 ccttgagttt gtctcgattg tctccatgat aacagaaaaa tagcggaaac cactaaagct
    44581 attgctatgc cctgtataga tgaaggcttc tcatcaaaag ccaagtaccc ccaaaacaac
    44641 ccggttattg caacgactcc accagttaaa ctatagaaaa caggtcctgc catacgaata
    44701 agaataaaga aaagcaaata tcctaaactg gataaaatta cttcaaggat gacgatttgt
    44761 ttcacgagag ttaacggagc tgagagagaa taaaatgcgt gctgcttgag gacaacaggg
    44821 gtaagcagaa gagacgccgc cagtaacatt ccattagcag cttgcagggc gttaatttct
    44881 tttggttgtt tggctcctat ataaattgag caaaaagcaa agccaagagg gctaagcata
    44941 gctaatatag tccagcttga gtaaagtcct gcggagttta ttcctataat tgcaagtatg
    45001 ccggacatac caagtaacaa ggcaaaaaat cgccacccat ccggtttttc ctggcccgcc
    45061 atgagagcta aagggtaaac cagtaaagga acagtattaa ctaacactgc caataaacct
    45121 gctgggatat ggctggcaat aaaatacatg ttggtattag gaatagcgat acctatcaat
    45181 ccacatataa aaaaataagg ccaataaaca gggtgtagca agcgagtttg ttttgtaaag
    45241 aagctcacta aagttaatag tagggctggc cctaatgatt gccaaaaagc atagccgagt
    45301 gggttaactc cattagtcat tgcaaatttg gcaagagtat agcctgatcc ccagatgaat
    45361 cctaaaacaa ttagaagaag gattgattgt atggttttta aatggagcat aaaaggtagg
    45421 ataaatagta taattttgga taattatgcc attgaaagtg gaaaataacg atatccttgt
    45481 gaggccttta aattttgctc aataaaacag ttttttaatc cctgcaaatg ctgaattatt
    45541 attttatatg attgcagtat attttgccaa aaatcctagt caagaacatg aagaatcgat
    45601 gatggaa
//
LOCUS       DASLOE010000006        45559 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.006, whole genome shotgun sequence.
ACCESSION   DASLOE010000006 DASLOE010000000
VERSION     DASLOE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 45559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45559
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.006"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..373)
                     /locus_tag="U6309_000260"
     CDS             complement(<1..373)
                     /locus_tag="U6309_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEN5503722.1"
                     /translation="MMELANKIIRVSYMDKLYQYLLLLRLSHWSKAIFVMLGFIYTPV
                     PGYLFPALMASLSFCLIASAVYIYNDIQDRTEDSLHPFKCNRPIASEQVTLFDAIIVM
                     LLLMVAGIALGWLISKKLVVIL"
     gene            703..1944
                     /locus_tag="U6309_000261"
     CDS             703..1944
                     /locus_tag="U6309_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0963 family Dot/Icm T4SS effector"
                     /protein_id="HEN5503723.1"
                     /translation="MRYILPAGALTSLNFLRDDYQSERKVDLDQPPSLIGVPTLFGGT
                     DVIARDKQTKFIKKMIVVLRANLAKEEEITTPEQWEANLTASRVVIAACLYVQSQISS
                     PKNNSALYRLINKDLGITDTNYLDDEDKEICFLAANRVVNSSLSAFSDANAALRKANM
                     KPFTEQEWSEFSKFVSSVSVKKVSTNPYSNYPVTSITQPLFGAAFSYTGATIGYLGGD
                     VISQSSKAMSTKLQITTMVGSSLLMLGTTGPMGVALFSQIIASKLVTAFFSISLAHVL
                     GTTMGILGQGVGMGIGLPLDYAYRLIWKASATIMNYYSNYPNTSQMTGIRIADGKSIM
                     NGIVIEYTPSSDLKTEEGQQTVEITEEGKLLINGKEVTIPDGGVQLPLELINELKAQL
                     KLQEEKVSEEDSSSSTLQATI"
     gene            complement(2030..5476)
                     /gene="dnaE"
                     /locus_tag="U6309_000262"
     CDS             complement(2030..5476)
                     /gene="dnaE"
                     /locus_tag="U6309_000262"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEN5503724.1"
                     /translation="MRQRFVHLRVHTEFSLVDGLVQIKPLMKALPARGMSTVAVTDFC
                     NLFAAVKVFKSATEAGIKPIIGSDLPCHDLETPDNIFSLVLLCQDTAGYKNLTCLVSK
                     AYQEGQYHGQPRVQLQWIPEFSRGLIALSGGKFGDIGQALLANDDTLARKRALHWMNL
                     FPNRFYLEIQRTGRADELHYNEKLIALADELQLPLVATNDVRFIDEDDFDAHEARVCI
                     HEGYTLADPRRVQQYSSQQYLRSADEMELLFSDLPSALQNTVEISKRCNVKLELGHNY
                     LPNFPIPDGTTVEAYLSHLSEVGLEERLKQLFRDKSEGELLAARGEYDKRLKIELDVI
                     NNMGFAGYFLIVADFIQWAKQNGVPVGPGRGSGAGSLVAYALKITDLDPLEYELLFER
                     FLNPERVSMPDFDIDFCMEGRDRVIEYVAEKYGRQSVSQIITFGTMAAKAVVRDVGRV
                     LGHPYGFVDKLAKLIPFELGITLSKAMEQEEELRRRYEEEEEVKELFDLALKLEGITR
                     NAGKHAGGVVIAPSQLTDFTAIYCEEGSTQIVSQFDKDDVEAVGLVKFDFLGLRTLTI
                     IDWALATVNKQCENAGLSPIDISQIPTSDDSTFELLKACKTTAVFQLESRGMKELISR
                     LQPDCFEDIIALVALFRPGPLQSGMVDDFIDRKHGRARVDYPHPDLEPILKPTYGVIL
                     YQEQVMQIAQVLANYTLGAADLLRRAMGKKKPEEMAKQREIFTQGAVARGVDEQVATH
                     IFDLMEKFAGYGFNKSHSAAYALVAYQTAWLKAHYPAAFMAAVMSSDMDNTDKVVTFI
                     DECAHMKLQVLPPSINNSMYHFTVSDEKTIIYGLGAIKGVGESAIECIIQERNQNGPF
                     LDLFGFCQRLDLRKVNRRVIEALIKSGAFDVWKVERAVLTASLEKALKVAEKEHQNQS
                     SGQFDLFSLLEDKADEQNYVESKSWSEAQRLEGEREVLGFYLTGHPADQYRRELGDFI
                     VPVSQLNPSLHKKAIICAQVVAIRKIITKRGKKLVIIGMDDATARIDAVVFGEIFEQY
                     SDPLATGNMLVIEGEVAIDDYNGGVKMTANQLLSIASARTKFARCLELRLNQNNAAVL
                     PSIQSLLKTYSGRCAVQLAYANGVAKANLNLGQQWCVTPSDELIGLLSNLLGEEQVIM
                     RY"
     gene            complement(5551..6897)
                     /locus_tag="U6309_000263"
     CDS             complement(5551..6897)
                     /locus_tag="U6309_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN5503725.1"
                     /translation="MTFELLWTDKCFLILLAVVLFTVLLSLRKQHIKLSFAIILQRPL
                     AISAGIVLLLFLAIGVLDSIHIKFINDKTEKTQSLLDRVLAPIGNTYEKTYSAPLALY
                     QFMSETKLVNGVTTQVYPRLKYPPASIKNEKDKSLIIKSVLMKGITYSALVILGGWLL
                     LALIKFSLGYKNIFILNSTQISAIATVAVLTFAVVIGYGLSRSFHVLGTGQIGQDIFY
                     FSLKSVRTGLIIGFLTTLFMLPLALFLGIAAGYFGGLVDDIIQYVYTTLSSIPGVLLI
                     TASVLSLQTYIANHPEQFTTLAKSADARLLALCLILGVTSWTSLCRLLRAETLKLREI
                     DYVLAARALGSSWFTIVRKHLVPNVMHIVIITLVLDFSFLVLAEAVLTYVGVGVSPMT
                     ISWGNMINGARLELARDPVVWWPMFAAFLLMFLLVLAMNLFADAVRDAFDPHQSHL"
     gene            complement(6921..7898)
                     /locus_tag="U6309_000264"
     CDS             complement(6921..7898)
                     /locus_tag="U6309_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN5503726.1"
                     /translation="MIKYILRRILYAIPILFGINLITFALFFMVNSPDDMARMHLGNK
                     HVEQKAIEDWKAVHGYDLPLFYNEKQTGFNQFRQTLFYQKSLMLFTFNFGVSDAGRDI
                     SHDISYRMWPSLAIALPTLLFAVLVNIIFAMAMAFFRSTYLDLSGVVICIILMSISSL
                     FYIIGGQYLFGKILKWFPISGYDDGLDALKFTILPVLVAVLGQLGAGARWYRTLVLEE
                     INKDYVKTARAKGLSEQRILFKHVLKNAMLPILTGIVVIIPTLFMGSLVLESFFGVPG
                     LGSYIIDAIQQQDFAIVRAMVFLGSVLYIIGLVLTDFSYILVDPRVRLS"
     gene            complement(7895..10060)
                     /locus_tag="U6309_000265"
     CDS             complement(7895..10060)
                     /locus_tag="U6309_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN5503727.1"
                     /translation="MQFMLFLMPCCVNLAYGSSWVFNNPYPESEANENIYYSSFNEQP
                     KTLDPARSYSSNEYLFIAQIYEPLLEYDYYKRPYELIPLTATRLPQIRYLDAQGNPVS
                     AGDNTKPAYSVYTIQIKKGILYQPHPALAQKKDGSYRYHHLPEDYLEEHDISKLSDFK
                     YTGTRELIIDDYIYQIKRLANPAVSSPIYGLMSYYILGFKEYGDSLPKGLANKNSFID
                     LRKYPLKGVKKLDDYTFEITLKGQYTQFLFWLAMPFFAPVPWEADLFYSQKDMDDKNL
                     SFGWYPIGTGPFMLTENNPNRSMILEKNPNFREVYFPVNGSDADRRAGYLTNQGRRLP
                     LIDKAVYTLEKESIPRWNKFLQGYYDMSGISADSFDQAIHINRFGEPILSQEMKDKKI
                     YLRQTLEPSIYYTGFNMLDSVVGGNSKRARKLRQAISIAVNFDENIAIFFNGRGIPAQ
                     GPIPPGIFGYKEGKEGINPYVYQWVNNARKRKSLKDAQKLMVEAGYPGGIDPKTGKHL
                     ILHYDVMTTGGPDDKARLDWMRKQFANIGIDLNIRATLYNRFQEKMRIGHAQIFSWGW
                     NADYPDPENFLFLLYGKNGKVKYGGENASNYENPEFDRLFNLMKNRPNDLQRQKLIDK
                     MLAIVRYDAPWVWGMHSEDFILSQDWVSNIKSNTISLNTLKYVSVNVPERNQLRRAWN
                     QPVFWPLGVLFGLLIALVIPLLITYLKKEKQPAERVKLK"
     gene            complement(10180..12201)
                     /gene="ligA"
                     /locus_tag="U6309_000266"
     CDS             complement(10180..12201)
                     /gene="ligA"
                     /locus_tag="U6309_000266"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEN5503728.1"
                     /translation="MNDQGIKESIETLKEQIRKYDYHYYVLDEPLVPDAEYDRCFKAL
                     QQYEEQYPQFLSPDSPTQRVSGTPSDAFMPVAHKQAMLSLSNVFTTDELKAFIKRAIE
                     KLDEPNQQLVFACEPKLDGLAVNMTYEGGILTHAATRGDGAVGENITANIKTIASVPL
                     RLRVSNPPKLIEVRGEVYIPKADFEAYNARARELGEKIFANPRNAAAGSLRQLNPEIS
                     ASRPLAIYCYGIGACEDYKLPNSHLEQLNLLKEFGFRVSPETRRAVGVEGCLDYYQYM
                     LAKRNQLPFEIDGVVYKIDSISLQQQLGYVSRAPRFACAHKFPATEEMTRLIAVDFQV
                     GRTGAVTPVARLEPVSVGGVTVSNATLHNFDEITRKDIHIGDTVIIRRAGDVIPEVVS
                     VILEKRPANARKIELPKNCPVCGSEVVREADEAIARCVGGLYCKAQLKRMMWHFASRK
                     AMYIEGLGSVLIDQLVDEGIVHHLADLYELDLQTLANLPRMGEKSAKNLLSALEKSKK
                     TTFNRFLYALGIREIGEAGARVLAEHYCDVESLKAATIEELMTLNDIGPVAASHIVHF
                     FAQAHNLEVIDRLLELGIHWPKPEKIQVNQQNPFFGKTVVLTGTLSTMGREEAKAKLL
                     ALGAKVSGSVSSKTDYLVAGSEAGSKLVKATELGVAIIEEDEFLKWVNS"
     gene            12423..13322
                     /locus_tag="U6309_000267"
     CDS             12423..13322
                     /locus_tag="U6309_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503729.1"
                     /translation="MELLRILSTLIILLFSTLIHSHSDTNSNQGIAFVHGTRDHREDA
                     YGGYWKIDFIQSISQTLAKPENHYVVHCDFSQYMWHEDAANCVANQLLEFINDKNVSS
                     LTVYTHSDGANIMRWILSNPTYDSRYMTLKRKISQVIAIAPSSGGTPLADEVLNGGIF
                     ETSLAWLLGYLSDAVKQQRVGDMLIYNEELLLGSKGRPSLSIPFKVIVGTDVYASPFS
                     TSSYCNGYMLNSGLKIAKLYLNQCADGFLNCDSQAQAGEVWFYDIDKTDYNLPLSHNQ
                     SRHSCFGLDQILISALVNEGVAK"
     gene            13319..14497
                     /locus_tag="U6309_000268"
     CDS             13319..14497
                     /locus_tag="U6309_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4785 family protein"
                     /protein_id="HEN5503730.1"
                     /translation="MKTAHLIWMSVFCIAQAHAFTLPRQPTKSYECENCSQLSRENLH
                     DKWEIIDEPLNNNISNARRSYGYKERISLEQLQRGVIISTLAPGAVVRITPLQNKSIP
                     ELLIKTPKSQLLPLKEASSLYNQDDEVGNNPLAITKHQAMLQIKPELGYGKFILKSKD
                     ITDKYADAYMISVLDKFSITYLEVETDSLHYQYGDKLKATISLHNDITEYDVNDVDAR
                     LVGPKGQVISLNLTKLKSNVFEGTATLDSELNDRGENWYLETDVQTEYGQEIIRRSGH
                     TAFSYSIPSASLMNVKKLSSKPLTFVVTVDVATASRYALQSVLFQKNGKGEARPIQTS
                     QRAQWLEPGKHVLQFTFDNHNQLSDDNLYLGYLRLIDYGQLKTVYQYNQPVKLSQLVD
                     "
     gene            14501..15763
                     /gene="rmuC"
                     /locus_tag="U6309_000269"
     CDS             14501..15763
                     /gene="rmuC"
                     /locus_tag="U6309_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEN5503731.1"
                     /translation="MALFSSVTLFEGIALAIAFQFLLLFWVLVKQAQQKKLTEQLGER
                     IQQTLISQLHQLHIDLSQSYQTSQQFINEKIAQGQLATQQLISDAIQRQMADVREQMT
                     HSFKHHASSLTSHLQLLTEEIRNHLNTLTQQVNHKLTEGFEKTSSTFIDVVKRLTIID
                     EAQKKITELSSHVVSLQDVLVDKKARGAFGEVQLSTLISNMLPSASYQMQYTLSNQKR
                     ADCVLFLPEPTGNIVIDAKFPLETYQRLINSDALSTDRKSLQQQFRQDIQKHIKDIAE
                     KYIVPNETTDGAMMFIPAESIFAEIHANYPDLIAMSQKLKVWLVSPSTLMAVLTTARA
                     VLKDDATRKQVHIIQKHLQALANDFQRFEKRMDKLSKHIDLAHQDVNEVNTSAKKITS
                     RFQKIESVDIELDEHEVTQLELVESESI"
     gene            15840..19301
                     /gene="mfd"
                     /locus_tag="U6309_000270"
     CDS             15840..19301
                     /gene="mfd"
                     /locus_tag="U6309_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEN5503732.1"
                     /translation="MNTKTLLYQPTKNKQIWGQLYGSSLALALAEYCQQTPGIKLLIT
                     QDNLCASQLQDELQFFLNPNQNTQELLFFPDWETLPYDQFSPHQDIISERLYTLSRVQ
                     QITNGIIISSASTLMHRLSPPEFLNQYALMLKEGQKLDLNSFRNQLQQAGYHCVNKVL
                     EHGEYALRGSIIDVYPMGSGLPFRIELFDDEIESLREFDPETQRTIEKINQIHLLPAR
                     EFPLNEQSQLHFRRAFRESFPGNPSHCPIYEAISNSQFPSGIEYYLPLFFDKTVTFFD
                     YLPANAKICLIEEIQENAERFWRELNERYEQRRHDVSRPILTPDNCFINPTELLTKAN
                     NYEQVRLYHKPTEKKGALNFNIAKAPQLPIDRKNIEPLRQLSEYCSDPTRRYLIVVES
                     AGRREVLLDLLKSSHIIPRTQHSWLDFIHDESPINITTGPLIYGCELTDKQIVIIVES
                     QLFGEQSTPQRRSTQKSVDPDLIIRDMAELRLGAPVVHLQFGVGRYQGLQYIDSNGTP
                     SEFLVLAYAGDDKIYVPVTSLHMISRYTGVDSEHAPLHKLGSDQWQKEKKKAAEKIHD
                     VAIELLDLYAKREAQPGHQYDFNQSEYIKFASGFPFTETPDQLQAIEQIIRDMQSPRP
                     MDRLICGDVGFGKTEVAMRAAFVAVQNGKQVCILAPTTLLAGQHFESFRDRFAEFPVN
                     IELLSRFRSNKESEAVLAALQSGKVDIVIGTHKLFQNNISFKNLGLLIIDEEHRFGVK
                     QKEHIKSLRTHVDILSMTATPIPRTLNMAMAGIRDISLIATPPAKRLAIKTFWQEKND
                     LTIREAILREILRGGQVFYLHNNVQTIERVCQDLEALVPEAKIQSAHGQMRERQLERI
                     MSDFYHHRFNVLVCTTIIETGIDIPTANTIIIDRADKFGLAQLHQLRGRVGRSHHQAY
                     AYLLTPNEKLLTPDAVKRLEAIVSLEDLGAGFTLATHDLEIRGAGELLGEEQSGNMHA
                     IGFNLFMEMLDRAVNDLKAGKTPELSAPMHQGPEIDLRISAIIPEDYIGDIHNRLIMY
                     KRIANAKTTQQLRELQIELIDRFGLLPQPVKHLFLITELKLKAAQLGIQKISASAQQG
                     KLDFSEKPSIDPGTLISLIQVHAKRYQMEGPQRLRFTLDSTSAEERIFEISSLLNKLS
                     PHSQTID"
     gene            complement(19280..20947)
                     /locus_tag="U6309_000271"
     CDS             complement(19280..20947)
                     /locus_tag="U6309_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEN5503733.1"
                     /translation="MQKESNSNSLFDLLLKNEKNSADRVYLRQPKNGVWHEYTWSSVM
                     LQARKVAGFLHHLGLKKGDHVSIISKNCAEWFIADFGIHIAGMVNVPLFANQNEESAH
                     YVLEHGDVKLVFVGKLDDHQRVRQFIPKSYPTIGFDYHKDLQVDYEWSDVLKSDPMIN
                     LVEPEPDALYTIIYSSGTSGAPKGAMYTNRSIANYLTVFPKDLLRIRELEHYKLISYL
                     PLAHVYERSAIQLGSVAINASVSFVESLEKFAENLREVSPTFFTAVPRIWGVFKQKIE
                     QKLPPAKLNFLLKIPLISSLIKRKIIHSLGLSECTNCFSGASHLPISIIDFFEKLGIY
                     IQEGYGQSENLAYATLSMLNERKRGYVGTPRLEVEIKVGEENELLMKCPCLMSGYYKN
                     EQATKNAFTEEGWLRTGDVVDVDNEDRVKILGRLSENFKNQTGEFIAPSPIEKLFSVN
                     PMLEQLCLVGRELPSNVLLVTLNEGVRLGMPKEEITHALQETLKQVNSKLVKYEKVSH
                     ILVLKDAWTTENDKLTPTLKVKRRVVEKDYAELIQKALQKSQSIVWE"
     gene            complement(20974..21852)
                     /gene="rssA"
                     /locus_tag="U6309_000272"
     CDS             complement(20974..21852)
                     /gene="rssA"
                     /locus_tag="U6309_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HEN5503734.1"
                     /translation="MTNQYPKIGLALGSGSARGWAHIGVIQSLKRLGVRIDCVAGCSI
                     GALVGAIYACGTLDLFEQWVLKLNKREMAKLIDVNFLGGGVLSGSKLMTFFKDFGLEK
                     NIEELSLPFSSVATDLSTGREIWLSKGSLELAIRSSMSLPGLFTPQRYKDCWLVDGGL
                     VNPVPITLCRAMGADVVIAVNLNHDLLRVPPSTTEHPGFFSVLSGQFDKMIKRIMGIS
                     DGPGYYDVLTRSIKIMQDRITRARAAGDPADLTLVPQLGNIMLYDFHKAKEAIHEGEN
                     CVLREKNNILQLVGLE"
     gene            complement(21973..23415)
                     /gene="tldD"
                     /locus_tag="U6309_000273"
     CDS             complement(21973..23415)
                     /gene="tldD"
                     /locus_tag="U6309_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEN5503735.1"
                     /translation="MTKSLAIAKNILLTPASLDEAGIEKLFKTMFNRNVDDADLYFQS
                     CSYESWYLEDSEVKSGNFSLDKGVGIRAVSGDKTGFAYCDDILLPSMLRAADAARSIA
                     LSGSKTVPAVKVLKASVNRYESLNPLEGMSKQEKIALLEAIDKEARSLDPRVIQVNAT
                     LTGCYEVVMVANMDGQMMADVRPLVSINVSVIVEDSHGRRESARSGGGGRVAYSYFTE
                     KENALSYAREAVREALTNLKAEPAPAGTMPVVLGPGWPGVLLHEAVGHGLEGDFNRKG
                     LSAFSGRLGQQVAAAGVTVVDDGTLENRRGSLTIDDEGTPSQCTTLIDNGILVNYMQD
                     KLNAKLMGMQSTGNCRRESYAHVPMPRMTNTYMLSGQYDPQEIISTVKKGLYAVNFGG
                     GQVDITSGQFVFSASEAYLIENGKITKPVKGATLIGNGPDVMKKISMIGNDLALDRGI
                     GVCGKEGQSVPVGVGQPTLKIDALTVGGTK"
     gene            complement(23427..24233)
                     /locus_tag="U6309_000274"
     CDS             complement(23427..24233)
                     /locus_tag="U6309_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEN5503736.1"
                     /translation="MNRVALVQMVSSAKIADNLQLTEQYLIQAREQEASLVVLPENFA
                     FMGMNEREKLHIAEYYGQGPIQQRISELARDLRVWIIAGTIPLKSMGSKVRASCIVYD
                     DKGLNVARYDKIHLFDVIVSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFP
                     ELYQYLTQRGAQLLSVPSAFTAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRH
                     TYGHSMVVEPWGKVLAQKEEGQGVILADIDLERLSQLRRQFPCVEHHVLM"
     gene            24452..25126
                     /locus_tag="U6309_000275"
     CDS             24452..25126
                     /locus_tag="U6309_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEN5503737.1"
                     /translation="MNRNDITILSQQRESVLATNKVLRNTYLLLSLTFIFSALTAYVA
                     FISGARPMNPLLMIVGVYGLMFLTQALRNSVWGLVSVFAFTGFLGYTIGPLLNYYITG
                     FSNGPQIVATALGGTGMIFFALSGYALTTKKDFSYLGGFLFVGVMVALLAMIAGIFIQ
                     IPALQLVISAAFVLISSGLILLQTSAIIHEGETNYIMATIGLFVSIYNLFVSLLNLLS
                     AFSGRE"
     gene            complement(25317..26285)
                     /locus_tag="U6309_000276"
     CDS             complement(25317..26285)
                     /locus_tag="U6309_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEN5503738.1"
                     /translation="MNSNYKREDFVNETEVRWCPGCGDYAILAALQRVLPELGLPPEQ
                     HVFVSGIGCAGRLPYYMNTYGFHTIHGRATAVATGLKAMREDLCVWVITGDGDALSIG
                     GNHLLHILRRNVNVNILLFNNQVYGLTKGQFSPTSQKGQVTKTSPKGVTSEPINPIMI
                     ALASGASFVARAVDKDPVHLASVLKKAYEHKGCSFVEIYQDCNIFNHGAFDDFAVKNN
                     RAENTIILEDGQPLVFGAQKEKALQLDDEQFVQISVDEKALYKHDAKSLISAMRLARL
                     TFPDYPVPLGVYYQKDRELFTLSQEVKKRPEDLPELFKAKASWKQN"
     gene            complement(26269..28086)
                     /locus_tag="U6309_000277"
     CDS             complement(26269..28086)
                     /locus_tag="U6309_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEN5503739.1"
                     /translation="MSTTDVIVVRITGDSGDGVQLVGEQLTISAALTGRDVRTMPDFP
                     AEIRAPAGTVAGVSGFQLAMAERAIFTAGESLDVLVALNPAALKNSLQHLNQGGLLII
                     NEDSFQERDWQKAGIDKSFLDNCREHYHIVAFPLITQTIQAVESINLTKPQAVKTKNF
                     YILGLVLWLFDLPTETCQAFIAKKFKTNQAIAKANELALLAGYNYAMTLELSRRDYML
                     GEVNRHSGEYRQITGVEAVGLALATLATQTQVPLLVSGYPITPASAILHECVRLSDYG
                     VQLIQAEDEIAAICSCIGAAYGGRLALTCTSGPGLDLKCESLGLAVVTELPLVLVDVQ
                     RAGASTGLPTKTSQSDLKQALYGRHGEAPLPVIAAQSPADCFNTIIEAFRIAIKYMTP
                     VIVLLDAYLANAAEPWEIPALDSLNIPKPEFNRFTKPYHRDEFLSRSWNVPGTSGFIH
                     QIGGLEKQGDEGRVSYDAENHQKMVITRAEKIKGIARDYPALVLEGDMTAPTLVIGWG
                     STFGSLKSAVHQCLEQGLPVAYLHLRHLNPLPDDLGSLLKKYDKVLVAELNSGQLCQL
                     IRAVYLVDAQSISQCNGQPFSVNHLVSAIKAEANYEQQL"
     gene            complement(28073..28819)
                     /gene="pdxJ"
                     /locus_tag="U6309_000278"
     CDS             complement(28073..28819)
                     /gene="pdxJ"
                     /locus_tag="U6309_000278"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEN5503740.1"
                     /translation="MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGIT
                     LHMREDLRHIQARDVRLIKQVLQTRMNLELAVTEAMLDFAEEISPEHACLVPEKREEL
                     TTEGGLDILTHRNVVEKAVRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCY
                     ADATSEEKQHYELQRIKEAAEFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHA
                     IIARALFCGLKEAVRHMRQLMQEARLYVND"
     gene            29108..29998
                     /gene="legL1"
                     /locus_tag="U6309_000279"
     CDS             29108..29998
                     /gene="legL1"
                     /locus_tag="U6309_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL1"
                     /protein_id="HEN5503741.1"
                     /translation="MVLTLSDLEKGYDKNLNQLSLSFLNLRDNDIPLLCEFLQNHPAI
                     TSLDLSHNDITANGMKLFVNKTSVSSLNISHNNIGPEGAQWLSEDNHITTLDVSFNEI
                     GDEGIKALAANAKLITLYALYNKITKVGAGYLAQSNLKKIDLCFNSLEDEGVIALASN
                     INIKELIASACDVSDIGAIELAKNNQLTLLILGKNAITDKSTLHFANNTSLSTLHLGS
                     NQITAAGKKILETNTRITDLDLIGNPIEVHETDELNYSKKSANSHSVFKFLQKFTFLS
                     CMSPCQETAESDKGLNKKPN"
     gene            30160..31335
                     /gene="ravJ"
                     /locus_tag="U6309_000280"
     CDS             30160..31335
                     /gene="ravJ"
                     /locus_tag="U6309_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavJ"
                     /protein_id="HEN5503742.1"
                     /translation="MAIAPQQIQERLKQEQYQKFVVADIGNFPHCLAQTPEGIASGQR
                     YQKYSTNSLSRTPPFSQWGAPQLLTPKSAQEYIKFAQQRNKKSSFKIDGEAVRVSECS
                     NFAYHAAGVLLDDPQIRTQYDVAVIGSMHSNGRYLHNITLLVPKGSRLPQPPQQLTAE
                     VFPTGTLIVDPWAVGMGHPPEQALAIPKEQFAYNRSLFPATVNYQSALDESLTSTRTG
                     QLTPYTGTPSQTGRQEVRPESQFPDTRRSVSATFPGNFAAYHELWRNAFQEIMNDPRH
                     QLHRNDVEYKKIHAIRTVLDDYTKGGDTWWAKFRRIFTFHWNRHHVKVVDDIVKEIDA
                     GNYTTSRALVDRLDNLAISLGSKLNPKGTLKEQIGFIKIQEQNPVEEIENLSRPHLT"
     gene            complement(31391..32080)
                     /gene="recO"
                     /locus_tag="U6309_000281"
     CDS             complement(31391..32080)
                     /gene="recO"
                     /locus_tag="U6309_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEN5503743.1"
                     /translation="MTSKSLNAWVIHKQWSGDTSARLKLFTKELGLINCLCKGGRTPK
                     KQSLLQAFIPLWVSIEERYDQYYTRNIESTSSRLDLEGHSLFSGLYINELLYYTLSPD
                     FPDPDLFDAYLFTLNGIALAREREAIEALLRRFEWALLKACGYTFSFLHEARTGELIV
                     PDSHYQFVAGEGFILGGDKKIPGEHLLAIAADNLSESAYLKSAKFIMRQAIDHLLGGR
                     EIKARSLYGPA"
     gene            complement(32070..32957)
                     /gene="era"
                     /locus_tag="U6309_000282"
     CDS             complement(32070..32957)
                     /gene="era"
                     /locus_tag="U6309_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEN5503744.1"
                     /translation="MSSYCGYIALVGRPNVGKSTLLNRILQQKLSITSRKPQTTRHSI
                     LGIRTEDEFQFVYVDTPGIHQGNAKAINRMMNKTAISVLRDVDVIAFLVDGTHWKDED
                     EYVLNLIKQAKVPCILVVNKVDKITDKTQLLPWIEQISQRYQFAAIIPLSAKTGLQVD
                     ELEGKLKTYLPEGPHLFPDDQFTDRSIKFLCAELLREKIFRFCGQELPYSVTVDIESF
                     KDEGNLIRIHALILVDKDNHKRMIIGDKGQKLKEMATNARLDMEKMLDKKVFLQCWCK
                     VKSGWSDDERILKQLGYDQ"
     gene            33219..36344
                     /locus_tag="U6309_000283"
     CDS             33219..36344
                     /locus_tag="U6309_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503745.1"
                     /translation="MPDFETTDGQTQVIIPLRVTPHTHKAMPTITGSIFSHEAGTVFY
                     GNYNMEYLLPGGDRLRLIANHNKKTVQLMLFTHNPELLKILPGDRPSSLVADVINHVN
                     RYKVQAESYKKPLSHEQEEKINLANACIRSLNHLLKKERQLKPHDHAGQEELRRKIIS
                     IIEHCQNDNRMIANNPVVSEGSFGYMLYDAHKTAQHYRFNRVFSVSRQDQMDFTKVTK
                     TQLGKPITPCFVWDSEIHIGHSSRELDDALRVICQQYQLCPAPALNNIPANRFAKLER
                     FISKLWHDGHDWINYLAVTTKPSHKTEIQKRSDGVSITKITPYYKLKGLPQIGYNSLA
                     AMVNHLVENSAQQTEVNSLKEAKRLLSHLPNGNWVIVPQKNTVLLRLENKLIHLKYFI
                     HDQLFYPLPDGEDLYILSQISKRHLYLPERISLRFKAFISRIPDFFKGFYKSMSQFII
                     HDLHEEFFNHVHATHAPKQHLSERDQRPKSKKIKRTSLHEALQNQGLLANGQTLEEFI
                     SDQINNSPYVIARANHPPTVHTYENPLHRALGVVRHLAGFFIDTSERNPMIGTLAMAA
                     YVYGAGAVLAPNVLTDILKKLHLSGLIYGIEPVQKLARLMNHGKISEAISASATLWQG
                     MVVSGNLDKFFVDAVSVLKEDPAEIAIIVSLALSLGYGLTKAIPSLQHEMGDFPYANY
                     AALGGKGGAAIYDTIMHPGDDWFLGTCKWFCKNIINIGKLLVAPFFEGFYYGFFDGFI
                     NGWKKSGKLIKQLGKQTLAASFDLILAILTIPLLEASSLLIHVPFRGITNFFRKLLSV
                     LGNFSVIGKLLIDIAERPSTNNFISEFTLSPLYGFTSPFGHFSDNPVTNIGINVIRLL
                     FLPLLQLIKNILILPLIDILSLTIRVNLSIINPISRLLAYSTGTILYHFGQIWDHSAG
                     VLFSTSAYGVTILCNHIDSKAGELKQYLLSLIEINRGKVYQWAFHDEDILTHKIVQDN
                     QYYFNDPRRCELIPHSDSHCLLRNLLLDEKSSTNKVKDNQKSQQLHFPGLFHGATQTS
                     DTMPQEHTLTI"
     gene            36586..38793
                     /gene="lidA"
                     /locus_tag="U6309_000284"
     CDS             36586..38793
                     /gene="lidA"
                     /locus_tag="U6309_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LidA"
                     /protein_id="HEN5503746.1"
                     /translation="MAKDNKSHQVKTSEGSLESVKTKEKEPVVEKMRVEDSKKEDKLS
                     MPTTKKESQPNEPAKPFKTSFEKWIESSLLDPQAKEDKGSTINLGREGLKNASQVKKF
                     LLSPAGKDVIAELGAQMALQRNINLQNQQDRMEHELFKRRLMAALFLWYLSKKSHAAE
                     KVKEIIREYNEKAIKNAEKASKPSQQSTSSTSQADKEIQKMLDEYEQAIKRAQENIKK
                     GEELEKKLDKLERQGKDLEDKYKTYEENLEGFEKLLTDSEELSLSEINEKMKAFSKDS
                     EKLTQLMEKHKGDEKTVQSLQREHHDIKAKLANLQVLHDAHTGKKSYVNEKGNPVSSL
                     KDAHLAINKDQEVVEHKGQFYLLQKGQWDAIKNDPAALEKAQKDYSQSKHDLATIKME
                     ALIHKLSLEMEKQLETINDLIMSTDPKENEEATKLLHKHNGLNLKLANLQDMLAVHRK
                     EKSFFNEKGEKVTSLNDAHYVIGKDQQLFNLGGKFYPIHKEQKILEKDGKFYLLKQGE
                     DWESIKDSPEKQKKAEHDFHKLQYETPMTVKKLVHHNKGLETTIHKERIEETKQQLED
                     NGKEKIEIANNISKLQSTVGVALNQLNQPTLDTESPVLTPSGGSTTVSPKPTPSLASV
                     TTFFKTKIDEMKKNEDVKVTLDDIMALSIKIADPKARTYFKNEVLKFAEQLKEVPRSG
                     AIPFQTMQRMLQTLEQYGVDTTKPSVTSIRSKTDEVLEQRFNPTPSLSPFKTS"
     gene            38969..39532
                     /locus_tag="U6309_000285"
     CDS             38969..39532
                     /locus_tag="U6309_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HEN5503747.1"
                     /translation="MIIEKILGNQSVIISLDIDNFLFDRLDQIANSDLDLVEINSSDT
                     NLLVKIKTQYPSLKIGAGGIIDTQQLEQCYQAGVHFASSPGFLASIAQTASVYSMCYF
                     PSVATISEAMAAANIGFKQVRPYPANLTFCNALSKYLPGLNLFPAEIDWNEAELFLNL
                     PAVAAVIIHNPDHKQLNGLARLAQSII"
     gene            complement(39829..40293)
                     /locus_tag="U6309_000286"
     CDS             complement(39829..40293)
                     /locus_tag="U6309_000286"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEN5503748.1"
                     /translation="MKKWPWLVLSFLVIICDQLSKYWVGVLLTPYKPMPVMPMLNFTL
                     AFNTGAAFSFLSGAGDWHRWFFAGFSFLMSIILLIWLVRTPEQARFQSVGISLILGGA
                     IGNLIDRGLHGYVIDFIDVYYKHHHFATFNLADSAICIGAAILVLDLLIRRE"
     gene            complement(40290..43085)
                     /gene="ileS"
                     /locus_tag="U6309_000287"
     CDS             complement(40290..43085)
                     /gene="ileS"
                     /locus_tag="U6309_000287"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEN5503749.1"
                     /translation="MAEYKDTLNLPNTSFPMKASLSVREPEMLADWQAKGIYKKIRKA
                     RDGSKRFILHDGPPYANGHLHCGHALNKILKDIIIKSKTFSGFDAPFVPGWDCHGLPI
                     ELNVEKKVGKAGSKISPREFRAKCREYAASQIDIQRDEFQRLGVLGDWYNPYVTMDYH
                     YEANIVRALGLMIKNGHLQQGFKPVHWCIDCGSALAEAEVDYEDKTSPSIDVAFSAVN
                     PSEFLNCFETQPAVKPLILPIWTTTPWTLPANEAVCLHPEIDYALIDAGNSYYIVATD
                     LVESVMARYGISHYKTSGSAKGRVFEHFKLQHPFYKRQVPVVLAEHVTTESGTGCVHT
                     APAHGPDDYLVGQFYQLPLINPVMANGCFAENVELFAGISVLKANETILAVLSERNVL
                     LASESIRHSYPHCWRHKSPMIFLATPQWFISMDKSNLRQAIINEIDKVNWVPDWGKAR
                     ISNMVENRPDWCISRQRSWGTPMPLFVHKTTRELHPDTLELIERVAMMIEKSGIDAWF
                     DLDSSELLGDDAKHYDKITDTMDVWLDSGISHYSVLKHNNDLDFPADVYFEGSDQHRG
                     WFNSSLTTAVAMYGVAPYKTVLTHGYTVDAEGKKLSKSKGNYVALDKLVNQHGADILR
                     LWVASTDYRHEVSISEEIIKRNADAYRRIRNTARFLLANLFDFNPASDCIEAKELLEL
                     DRWALKRCQLLQEEIITAYENYHFHLIYQKIHNFCAVDMGSFYLDLIKDRQYTTAKDS
                     IARRSCQTAMYHMVKAFTIWLAPILSFTAEEIWQTIPGNNSESIFIEHWYNAWPTIDA
                     VNMEDWEQLHIVRDEVNKALEETRQRGEIGSALAAEVTVYADAKVLPKLTRLGEELRF
                     LFITSEAKACPISQSPKGLAVTDCGVSIQVTASVHEKCARCWHRREDVGQNQEHPELC
                     LRCVGNISGYHEERLYI"
     gene            complement(43198..44184)
                     /gene="ribF"
                     /locus_tag="U6309_000288"
     CDS             complement(43198..44184)
                     /gene="ribF"
                     /locus_tag="U6309_000288"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEN5503750.1"
                     /translation="MKLLRSSTHFAIFDKGAVATIGNFDGVHLGHQHLIKALRAKADE
                     MNLPLVILLFEPQPKEYFHREKAPARLSTLREKIDVLNLCQVDYIYCIKFDARLAQTP
                     ALYFAQFYLFEALKIRYLLVGQDFRFGKNREGDVNLLKTLGANYSCEVTVQSDFLIEN
                     EKISSTRIREALQQGNLRFAAKLLGRPYSMCGRVIKGDGRGRQWGIPTANLGIHRLSI
                     PLQGVYAVQVHINSNVFYGVANIGKRPTVAGTKNILEVHLFDFHGSLYGQLVQVIFLH
                     KLRDEVKFSSVEALITQINSDIILAKTYLKQNWLDSAIDSSNDVVNGISNTV"
     gene            complement(44308..44739)
                     /locus_tag="U6309_000289"
     CDS             complement(44308..44739)
                     /locus_tag="U6309_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN5503751.1"
                     /translation="MYKRVLFATDFDEVGVRAAEKAKKIADENGADLILVHVVEPIPA
                     YAYPGFAGFAEVEMSIREQAEKELNELAEKLGVDEKHRFIEYGSTKNEILRVAKEHKI
                     DLIVTGSHGKHGLSLLLGSTANAILHGAECDVLIVRPKISH"
     gene            complement(44848..>45559)
                     /locus_tag="U6309_000290"
     CDS             complement(44848..>45559)
                     /locus_tag="U6309_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEN5503752.1"
                     /translation="ISLVASFAIVAGLSYFYFDNVYKAPDNTLKLPDLTSVTTLPPIN
                     DLIKTPTQQAVPKQESVSYIASWEDSSTRQLVHYGLPKKQILDDFMDEEDSLNTVAVV
                     DKVVVIPKLKAKNISEQVGQAVVSQSKLPPIPHSSQTKLASEQPGIKKINSKLFTIQL
                     AASHKVEDINRFKNQNKWLAQAKIRHFTNAKGSWYILTIGEYESRNQALLNIKKLPGE
                     LAKLSPWIRSVEGLSNIG"
BASE COUNT        14433 a         9993 c         7907 g        13226 t
ORIGIN      
        1 ttaaaatgac caccagtttt tttgaaataa gccaacccag cgcgattcca gcaaccatca
       61 ataaaagcat tacaatgatc gcatcaaata aagtgacctg ctcgctggct ataggtctgt
      121 tgcatttaaa gggatgcaga ctgtcttccg ttctatcctg aatatcatta taaatataaa
      181 cggcacttgc aataagacaa aaagataatg aagccattaa tgccggaaac aaataccctg
      241 gaacaggagt ataaatgaaa ccgagcataa caaaaattgc tttactccag tgcgatagac
      301 gaagaagtaa aagatattgg tataacttat ccatgtaact taccctgatg attttatttg
      361 ctaattccat catacaaatc tacccttttc tcaaaaacag ctaaaacagt tctgtcattt
      421 ctaatcaaag tattaatgac cgcctcaatc aggctacagg acatggattt gttttaattt
      481 taaattaatt acaccctata agagtaacat atctaactca aggctaaaaa tagtataatc
      541 gcaaaaaaat catcatttca aagagttaaa tcataaataa aatctgttgc cttatctgat
      601 tgcaacaatt tagtaaaaat taaccaaatc agattgcatt taaccagcta atcggtaaga
      661 taaaaaacac acaaataaat tttcaatgga tatgaggtta ttatgcgcta tatactgcca
      721 gcaggagctc taaccagcct taacttttta cgcgatgact atcaatcaga aagaaaagtg
      781 gatttggatc aacctccatc tttaatcgga gtacctaccc tttttggagg cactgatgtc
      841 atagccagag ataaacaaac caaatttata aaaaaaatga ttgttgtcct tagagcaaat
      901 ctggcaaaag aggaagaaat cacaacccct gaacaatggg aagcgaattt aactgcttct
      961 agagtcgtca tcgcagcctg tctatatgtt caatcccaaa tttcatctcc caaaaataac
     1021 tccgcattat atcgattgat taataaggat ttaggcatta ctgataccaa ttatctggat
     1081 gatgaagaca aggaaatctg ctttttagct gccaatcgag tggtcaactc ctctttatcc
     1141 gcatttagcg atgccaacgc tgccttgcgc aaagccaata tgaagccatt taccgaacag
     1201 gagtggagtg agttttccaa gtttgtcagt agtgtgagtg ttaaaaaggt atctaccaat
     1261 ccttactcga actatcccgt cacatccatt actcaaccgt tattcggagc cgctttttct
     1321 tatacaggag caacgattgg ttatcttggg ggagatgtta ttagccaatc ctccaaagcg
     1381 atgtcgacca aactacaaat tactaccatg gtgggaagca gcctgctgat gctcggtaca
     1441 acagggccta tgggtgtggc gttgttctct cagatcatag caagtaaatt agtcacggca
     1501 tttttcagta ttagtttggc tcatgtttta ggaacaacca tgggtattct cggacaaggc
     1561 gtaggaatgg gcattgggtt acctttggat tatgcctaca gattaatatg gaaagccagc
     1621 gccactatta tgaattacta cagcaactat cctaatacct cacaaatgac gggtattcgt
     1681 attgcggatg gcaaatccat catgaatggc atagtgattg aatacactcc aagctctgat
     1741 ttaaaaactg aagaaggaca gcaaacagtt gaaattactg aagaaggaaa acttcttatc
     1801 aatggaaaag aagtcactat tcctgacggc ggagttcaac ttccactaga gttaattaat
     1861 gaattaaaag ctcaattgaa gctgcaggaa gaaaaagtta gcgaagaaga ctcttcctct
     1921 tccacattgc aagcaactat ttgatgtaga tgagacaact atcattctgc aatgcgagtt
     1981 gagctgcgct atctgctcga ctccacaggc gctatcatta gcagtatttc taataacgca
     2041 ttattacttg ctcttcccct aaaagattgc tcaatagtcc aattaactca tcgctagggg
     2101 tcacacacca ttgttggcca agatttaaat tagctttagc aacaccatta gcataagcca
     2161 attgaacagc acaccgtcct gaataagtct tcagtaagga ttggatagaa ggcaatacag
     2221 cagcattgtt ttgatttaaa cgcaactcca gacaacgagc gaattttgtt cgagctgaag
     2281 cgatacttaa aagctgattg gcggtcattt taacaccacc gttgtaatca tcaattgcaa
     2341 cctcgccctc aattacaagc atgttacccg tcgccaatgg atcagaatat tgttcaaaaa
     2401 tctcaccaaa aactacggca tcaatcctgg cagtcgcatc atccattcca ataatcacta
     2461 atttcttgcc acgtttggtg ataatttttc taattgctac aacctgagcg caaataattg
     2521 cttttttatg taaagacgga tttaattggc tgacaggaac aataaaatct cccaattcac
     2581 gtctgtattg gtcagcagga tggccagtga ggtaaaaacc taatacctct ctttcgcctt
     2641 ctaatctttg ggcttcagac caagatttac tttccacata attttgttca tccgctttgt
     2701 cctctaaaag ggagaataga tcaaattgcc cacttgattg attttgatgt tccttttcgg
     2761 caactttcag tgctttttcc aaggacgctg ttaatacagc cctttccacc ttccaaacat
     2821 caaaagcacc actcttgatt aaagcttcaa tcactcttct gttgactttg cgtaaatcca
     2881 atctttgaca aaaaccaaac aaatccaaaa aaggtccatt ctggtttctc tcctgaataa
     2941 tacactcaat cgcagattcg ccaactccct ttattgcccc aaggccataa ataatcgtct
     3001 tttcatcgga cacggtaaaa tgatacatgg agttattgat agaaggaggc aacacttgta
     3061 atttcatgtg cgcgcattca tcaataaaag ttacaacctt atccgtgtta tccatatcag
     3121 aggacatcac cgcagccata aaagctgcag gataatgtgc ttttagccag gcggtttgat
     3181 aagcgactaa agcgtacgcg gcagaatgtg atttattgaa tccatatccg gcgaacttct
     3241 ccattaaatc aaaaatatgg gttgctacct gctcatctac tcctctcgca acagctccct
     3301 gggtaaaaat ttccctttgc tttgccattt cttccggttt tttcttcccc atggctcgtc
     3361 ttaacaagtc agcagcgcca agcgtgtaat tggcaagaac ctgagcgatt tgcattactt
     3421 gttcttgata gaggataacc ccataagtgg gtttaagaat tggttccaag tctggatggg
     3481 ggtaatcaac tcgagccctg ccatgcttcc tgtcgataaa gtcatccacc atccctgact
     3541 gtagaggacc tggtctgaat aaagcaacca aagcaatgat gtcttcaaaa caatcaggtt
     3601 gaagccggct aatcagctcc ttcatgcctc gagattccag ctgaaataca gcagtggttt
     3661 tacatgcttt taataactca aaagtagagt catcactcgt tggaatttga ctaatatcaa
     3721 ttggtgacaa cccggcattt tcgcattgct tattcactgt ggccagagcc caatcgatta
     3781 tggttaatgt tcttaatcct aaaaaatcaa acttgactaa cccaaccgcc tcaacatcgt
     3841 ctttatcaaa ttggctaact atttgtgttg atccttcttc acagtatatc gctgtgaaat
     3901 cagtcagctg agaaggtgca ataactactc ccccagcatg tttgcctgca ttacgagtaa
     3961 ttccctccag tttcagggca agatcaaata gctcttttac ttcctcctct tcttcatatc
     4021 gccggcgaag ctcttcttcc tgctccatcg ctttacttaa agttattcct aactcaaagg
     4081 gaattaattt cgcaagctta tccacaaagc cataaggatg acctaaaaca cgcccaacat
     4141 ctctcaccac agcctttgcg gccatagtgc caaaagtaat aatttgggaa acgctctgcc
     4201 ggccatattt ctctgctaca tattcaatga cccgatcacg gccttccatg caaaaatcaa
     4261 tatcaaagtc aggcatggaa acacgttcgg ggtttaagaa tcgttcaaaa agcagctcat
     4321 actctaaagg atccagatct gttattttta gggcataagc aaccagagaa ccagcaccag
     4381 aaccacgacc tggtcctact ggaaccccat tttgtttagc ccattgaata aaatcagcaa
     4441 caatcaggaa gtaaccagca aagcccatgt tattaataac atccagttca attttaaggc
     4501 gtttgtcgta ctcccctctg gcagcaagca gctccccctc agacttgtct ctgaataatt
     4561 gctttaaacg ttcctccaag ccaacttcag aaagatgtga taaatacgcc tctaccgttg
     4621 ttccatccgg tatagggaaa ttaggtagat agttatggcc cagctcgagt ttcacattac
     4681 agcgtttact aatttcgact gtgttttgta atgcactggg aagatccgaa aataatagct
     4741 ccatttcatc tgctgagcgc agatactgtt gggagctgta ttgttgaact ctcctgggat
     4801 cagccaacgt gtacccttcg tgaatgcata cacgagcttc atgagcatca aaatcatcct
     4861 catcgatgaa tctaacgtcg ttggttgcaa ccaaaggtaa ttgcaattcg tccgctaacg
     4921 cgatcagctt ctcattataa tgcaactcat cagctcttcc ggttcgttga atttccagat
     4981 aaaaccgatt cggaaataaa ttcatccaat gcaaagcacg ttttcttgcc aaggtgtcat
     5041 catttgccag caatgcttgc ccaatgtcac cgaactttcc tccggataat gcgattaatc
     5101 cccgtgaaaa ttctggaatc cattgtaatt gcaccctggg ttggccatga tattgccctt
     5161 cctgatatgc tttggatacc agacaggtta aatttttata gcctgctgtg tcttgacata
     5221 acaaaaccaa agaaaaaatg ttatccgggg tttccaaatc atggcatggc aaatcactac
     5281 caataatggg tttgattccc gcctctgtcg cacttttaaa tactttgact gcagcgaata
     5341 aattacaaaa atccgttaca gcaactgtgc tcatacctct ggctggcaaa gctttcatca
     5401 atggtttaat ctgtaccaaa ccatcaacta atgaaaattc cgtatgtact cttaaatgaa
     5461 caaaacgttg tcgcatagag aattaaccca ttcacgataa tttcgaaact ttaacctaaa
     5521 tcccggaaat aggaaactaa cagatattga ctataaatga gattgatgtg ggtcaaaagc
     5581 atcccgtact gcgtctgcaa acaagttcat tgccaaaacc aataaaaaca ttaacagaaa
     5641 ggctgcaaac ataggccacc atacaacagg gtcacgtgct aattcaaggc gcgccccatt
     5701 aatcatattt ccccaactaa tagtcatggg tgaaacccct acccctacat aggtcaatac
     5761 agcttctgcc aagactaaaa aactgaaatc caataccagg gtgatgataa caatatgcat
     5821 gacattgggc accaaatgtt tgcgtacgat ggtaaaccaa ctactgccta aggcacgtgc
     5881 ggccaggaca taatctattt ctcgcaattt taaagtttct gctcgcaaaa gacggcacag
     5941 gcttgtccaa ctggtcacac ccagtatcaa gcaaagagct aataaacgtg catcggcact
     6001 cttggccaaa gtagtaaatt gctctgggtg gtttgcaata taagtttgca atgacaatac
     6061 ggaagccgta attagcaaaa cacctggaat ggagcttaaa gtagtataaa catactggat
     6121 aatatcatcc acaagcccac caaaataccc tgctgcaatc cccagaaata aagccagtgg
     6181 caacatgaag agggttgtta aaaaaccaat gattagaccc gttcgcacac ttttcaaact
     6241 gaaataaaaa atatcttgcc cgatttgccc tgtgcccaag acatgaaagg atcttgataa
     6301 tccgtaacca atgacgaccg caaaagtaag tacagctaca gtcgctatag cgctgatttg
     6361 agtagaattt aaaatgaaaa tatttttata tccaagacta aactttatta aagcgagtaa
     6421 taaccaccca cccaaaatga ctaaagcact atatgttata cctttcataa gcacagactt
     6481 gatgataagg gatttgtcct tttcattctt aatagaagct ggtggatatt ttaagcgagg
     6541 ataaacctga gttgtgactc cattaaccaa cttcgtttca ctcataaatt gatataaagc
     6601 caaaggagct gaataagttt tctcataggt attccctata ggtgcaagaa ctctgtccaa
     6661 tagagactgc gttttttctg ttttatcatt gatgaattta atatgaatgg agtccaaaac
     6721 accgatggct aaaaacagca acaacacaat tccagcactg attgctaagg gcctttgtaa
     6781 aatgattgca aatgacaatt tgatgtgttg ttttctaagg ctcagcaaaa cggtaaataa
     6841 aaccaccgcc agtaaaataa gaaaacattt gtcagtccaa agcaattcaa atgtcatttt
     6901 tgccacacac tccttggtta tcaggataat cgcactcggg gatcaactag gatgtaagaa
     6961 aaatcagtca gaaccaagcc tatgatgtaa agaactgacc ccagaaatac catagctcgt
     7021 acaatagcga aatcttgctg ttgtatcgca tcaataatat agctacccaa gcctggcaca
     7081 ccaaaaaaag actctaaaac caaactcccc ataaacaaag taggaatgat tacaacaatt
     7141 ccggtcaaaa tgggtaacat ggcatttttt aatacatgct taaataaaat cctttgctca
     7201 gagagaccct ttgctcttgc agtttttaca taatctttat taatttcttc caaaaccaag
     7261 gttcgatacc aacgcgctcc cgcccccaat tgtcccagaa cagctaccag gactggtaaa
     7321 atagtaaatt ttaaagcatc gaggccatcg tcgtagccgg aaatagggaa ccattttaat
     7381 atttttccaa acagatattg cccaccaatg atgtaaaata aactggaaat ggacattagg
     7441 ataatacaaa taactacccc ggataaatca agataagtcg aacggaaaaa tgccatagcc
     7501 atagcaaaaa tgatattcac caaaacagca aataataatg ttggcaaggc aatagcaagg
     7561 cttggccaca ttctgtagga tatgtcgtgg ctaatatctc taccagcatc cgagacacca
     7621 aaattaaatg taaacagcat cagcgatttt tgataaaaca aagtctgtct aaattgatta
     7681 aatccagtct gcttttcatt ataaaataac ggtaaatcat agccatgaac cgctttccaa
     7741 tcttcaatgg ctttttgctc tacatgttta ttacccaaat gcatacgtgc catatcatcg
     7801 ggagaattaa ccatgaaaaa caatgcaaaa gtaataagat taataccaaa caaaattgga
     7861 atcgcatata aaattctgcg gagtatgtat ttaatcattt taattttact ctttctgccg
     7921 gctgtttttc tttctttaaa taagtaatca ataaagggat taccagagca atcaataggc
     7981 caaacagcac acctaaaggc caaaataccg gttgattcca ggctcttcgc agctggttac
     8041 gttcaggtac attaacagaa acatatttta gagtattgag agaaatcgta ttggatttta
     8101 tattagatac ccaatcttgc gataaaataa agtcttcaga atgcatgccc catacccatg
     8161 gggcatcata ccggacaatc gctaacatct tatcaatcag tttttgtctt tgtagatcgt
     8221 tagggcgatt tttcatcaaa ttaaacaatc tatcaaattc aggattttca taatttgatg
     8281 cattttcacc accatacttc acttttccat ttttcccata aagcaaaaat aaaaaatttt
     8341 cgggatcagg ataatcggca ttccagcccc aactaaatat ttgagcatga cctatccgca
     8401 tcttttcctg gaaacgattg taaagagtcg ccctaatatt caaatctatc cctatatttg
     8461 cgaattgctt tcgcatccag tcaagccttg ctttgtcatc aggtccacct gtcgtcatga
     8521 catcataatg aagaatcagg tgttttccgg ttttcggatc tatcccaccc ggatatcctg
     8581 cctcaaccat taacttctgc gcatccttta aggattttcg tttcctggca ttattcaccc
     8641 attgataaac atagggatta ataccctctt ttccttcctt gtacccaaag ataccaggtg
     8701 gaatagggcc ttgcgccgga atacctcggc cattaaaaaa tatggcaata ttttcatcaa
     8761 aattcaccgc aatagaaatt gcctggcgta attttcgtgc tcttttgcta ttgcccccaa
     8821 caacgctgtc aagcatatta aagccagtat aatagataga cggttccagg gtttgtctta
     8881 aatatatttt tttatctttc atttcctggc taaggatagg ttctccaaag cgattaatat
     8941 ggatagcctg atcaaaactg tcagcactta tccccgacat atcatagtag ccttgcaaga
     9001 atttattcca tcgaggtatg gattcctttt caagggtata aacggcttta tcaattaagg
     9061 gcaagcgcct tccctggttg gttaaataac ctgcccgtct atcggcatct gaaccattga
     9121 caggaaaata cacttctctg aaattaggat ttttctccaa tatcatgctt ctattgggat
     9181 tattttcagt cagcataaaa ggccctgtac ctataggata ccagccaaaa gataagttct
     9241 tatcatccat atctttttgg gaataaaata agtccgcttc ccaaggaacc ggagcaaaaa
     9301 agggcatggc taaccaaaac aaaaattgtg tatattgtcc ctttaatgta atctcaaagg
     9361 tgtaatcatc taatttttta acccccttca aaggatactt acgtaaatca ataaaagaat
     9421 ttttattggc caatccttta ggaagactat ctccatactc cttgaagccc agaatgtaat
     9481 agctcatcaa tccatatatc ggagaactca cagcagggtt tgccaatctt ttaatttgat
     9541 aaatgtaatc atcaatgatc agctcccttg taccggtgta tttaaaatct gacaacttgc
     9601 taatatcatg ctcttccaaa taatcttctg gtaagtgatg atatcgataa gagccatctt
     9661 tcttttgagc caaagcagga tgaggttgat ataaaattcc tttttttatc tgaatggtat
     9721 agacactgta tgctggcttt gtgttatctc ctgccgaaac aggatttccc tgggcatcca
     9781 aatatcgaat ctgtggtaag cgggtagctg ttaatgggat taattcatag ggtcttttat
     9841 agtaatcata ttccagcagt ggctcataga tctgggctat gaaaagatat tcattggaag
     9901 aataggaacg tgccggatcc agggtttttg gctgttcatt aaaagaagaa taataaatat
     9961 tttcattggc ctcgctctct gggtaaggat tattgaatac ccaagaactg ccataagcca
    10021 aattgacaca gcaaggcatt aaaaataaca tgaattgcat taacaaatat cgccgcactt
    10081 acttccctcg tgccaatcag tcattattac aggtttaaat acctacttca cccgattcat
    10141 tgatacccat cataaaccaa ataacaaaga tctagctact catgaattaa cccattttaa
    10201 aaactcgtct tcctctataa tcgctactcc cagttctgtc gctttaacca gctttgaacc
    10261 ggcttcactt cctgctacta aataatccgt tttggaagac acacttccac tcacttttgc
    10321 gcctaaggct aataattttg cctttgcctc ttccctcccc atggtactca gagttccagt
    10381 taaaactact gttttaccaa aaaatggatt ttgctgatta acctgtattt tttcgggctt
    10441 aggccaatga atgcccaact cgagaagacg gtcaatcact tcaagattat gcgcttgggc
    10501 aaagaaatgg actatatgag aagccgctac tggacctata tcattcagag tcattaattc
    10561 ctcaatcgtt gccgctttca agctctctac atcgcagtag tgctccgcta aaacccttgc
    10621 gccggcttca ccgatttctc tgatccccaa agcataaaga aagcgattga aagtagtctt
    10681 tttacttttt tccaaagcgg ataataggtt ttttgcagat ttctccccca tccttggcag
    10741 gttagctaaa gtctgcaaat cgagttcata aagatccgcc aaatgatgga caataccctc
    10801 atcaactaac tgatcaatta aaacactgcc aagtccttca atatacatcg cttttcgaga
    10861 agcaaaatgc cacatcatcc tttttaattg tgctttacaa tataaaccgc cgacacaacg
    10921 agcaattgct tcatccgctt cccttacgac ttcagaacca caaacagggc aatttttagg
    10981 aagctcaatc tttctggcat tagcgggacg cttttccaaa atcacagaaa ccacttcagg
    11041 gatcacgtca ccagcgcgac gaataataac cgtatcacca atatgaatgt cttttcgggt
    11101 aatttcatca aaattatgca aagtcgcatt acttactgta acaccaccaa cactaactgg
    11161 ctccaaacgt gcaaccggcg tcacagcgcc cgttctaccc acctggaaat ccacggctat
    11221 caggcgagtc atttcttctg tagcgggaaa tttatgggca caagcaaatc ttggggcacg
    11281 agaaacataa cctaattgct gttgcaagga gatactgtca atcttataaa caaccccatc
    11341 tatttcaaat ggcaattgat tccgtttcgc taacatatac tggtaataat ctaaacagcc
    11401 ttctactcca accgccctcc tcgtttctgg agacactcta aatccaaact cttttaataa
    11461 attcaattgc tccaaatgac tgttaggtaa cttataatcc tcgcaagccc ctataccata
    11521 acaataaata gcaagtggac gactggcaga aatttcagga tttaattgcc ttaaactgcc
    11581 tgcagcagca tttcgcggat tagcaaaaat tttttcaccg agttctctag cccttgcgtt
    11641 gtatgcttca aaatcggctt tggggatata gacttcaccc cgcacttcga tcaattttgg
    11701 agggttacta acccttagcc ttaatggaac tgaagcaata gtcttaatat ttgctgtgat
    11761 gttttcacct acagcaccat cgccacgagt tgcggcatga gtcaagatcc cgccctcata
    11821 agtcatgtta acagccaacc catcaagctt tggttcgcaa gcaaatacca gttgttgatt
    11881 tggttcatcc agtttctcaa ttgctcgctt aatgaatgct tttaattcat cggtagtaaa
    11941 cacattggac aaagacaaca tggcttgctt atgggctacc ggcataaaag catctgaagg
    12001 agtaccgctc actctctgtg taggagaatc tggcgataaa aattgcggat attgctcttc
    12061 atactgttgc aatgccttga aacatcgatc atattccgcg tcaggaacca aaggttcatc
    12121 taaaacataa tagtgataat cgtattttct tatttgctct ttaagcgttt ctatcgattc
    12181 cttaattcct tgatcattca ttaatgaaga ttcccaaaat aaaaacaaca gtttatcatt
    12241 tttttacttc tttcaaatat gaatggttta atcattcaag gcaaaatctt tttttggaag
    12301 cctctttatt cggaaccaag taatcggcgc aaatccacta aaacgcatca atcccatctc
    12361 ttgaatcttt tgccagaacc atgatataga tacgtctctg tattgaagga gatattttga
    12421 aaatggaact tctgcgcatt ttatccactc ttattatttt attgttttca acccttatcc
    12481 attctcattc cgacactaat tccaatcaag gcattgcttt tgtccatggt actcgagatc
    12541 acagagaaga tgcctatgga gggtattgga aaattgattt catacaaagt atctcccaaa
    12601 ccctggcaaa acctgaaaat cactacgtag ttcattgtga ctttagccaa tatatgtggc
    12661 atgaagacgc agccaattgt gttgccaatc aacttctgga atttattaac gataaaaatg
    12721 tgagctctct cacagtctac acccactctg atggtgccaa catcatgcgt tggattctct
    12781 caaacccaac ttatgatagc cgatatatga ctctcaaacg caaaatcagt caagtgattg
    12841 ccattgcacc ttccagtgga gggactcctc ttgctgatga agtattaaat ggcggcatat
    12901 ttgaaacgag tctggcctgg ttactcggtt atctttcaga tgcagtcaaa caacaacgtg
    12961 taggcgacat gctgatctat aacgaagaat tgctattggg aagcaaaggc cgaccctctc
    13021 tgtccattcc atttaaagtc atagtgggta ctgatgtcta tgcttcacca ttttcaacat
    13081 ccagctattg taatggatac atgctaaata gcgggctaaa aattgccaaa ctctatctta
    13141 atcagtgtgc tgatggcttt ctgaattgtg attcgcaagc tcaagcagga gaagtatggt
    13201 tctatgatat agacaaaact gattacaatt tgcctttaag ccataatcaa agtcgacatt
    13261 cttgttttgg tttggatcaa atacttatca gtgccctagt taatgaagga gttgcaaaat
    13321 gaaaactgcc caccttatat ggatgtccgt attttgtatt gcccaagctc atgcgtttac
    13381 attaccaaga caaccaacca aatcttatga atgtgagaat tgcagccaat tatcacgaga
    13441 aaatctgcac gataaatggg aaattattga tgaaccatta aacaacaaca tcagtaatgc
    13501 tcgtagaagt tatggttaca aagaacgtat ttctctggaa caattacaac gcggagttat
    13561 tatatcaaca ttggctcctg gcgctgtcgt tcgcattacc cctttgcaga ataaatccat
    13621 cccggaatta cttatcaaaa cgccaaaaag ccaactcttg cccctgaaag aagcctcttc
    13681 tctctataat caggatgacg aggttggtaa taatccttta gccattacaa aacaccaggc
    13741 tatgttacaa ataaaacctg aactggggta tggaaaattt atactaaaaa gcaaggacat
    13801 tactgacaaa tatgccgatg cttatatgat cagtgtcctt gataaatttt ccatcactta
    13861 cctggaggtt gaaaccgatt ctctccatta ccagtatggc gataagctaa aagctaccat
    13921 atcgcttcac aatgatatca ctgagtatga cgtcaatgac gttgatgctc gacttgtagg
    13981 ccctaaaggt caagtcattt cgcttaatct aaccaagtta aaatccaatg tatttgaagg
    14041 aactgccact ttggattccg agttaaatga tcgcggcgaa aactggtact tagaaactga
    14101 tgttcaaaca gaatacgggc aagaaatcat tagacgatct ggccatacag ccttttcata
    14161 ctccattccc tcagccagtc ttatgaatgt aaaaaaattg tcctctaagc ctttgacttt
    14221 tgttgtcaca gtagatgttg caactgcaag ccgatacgct ttacaaagtg tgttgttcca
    14281 aaaaaatgga aagggtgaag ccaggcctat tcagacctcg caaagagctc agtggcttga
    14341 gcccggtaag catgttttgc aatttacctt tgacaatcac aatcaattat cggacgataa
    14401 cctctatttg ggttatttac gcttgataga ctatgggcag ttaaaaactg tgtatcaata
    14461 caaccaacct gtcaagctta gtcagttagt ggattaatga atggcattat tttcttctgt
    14521 cactttgttt gagggcatag cgttggctat tgcctttcaa ttcttattgc ttttctgggt
    14581 tttggtaaaa caagcacagc aaaaaaaact gaccgaacaa cttggtgaaa gaatacaaca
    14641 aacactaatc tctcaacttc accagttaca tattgattta agccaatcat atcaaacgag
    14701 ccaacaattt attaatgaaa agatagctca aggacaactg gctacacaac agctcattag
    14761 tgatgccata caaagacaaa tggccgatgt cagagagcaa atgactcata gcttcaaaca
    14821 tcatgcgagc tcactcactt ctcacttaca attactaact gaggaaattc gtaatcattt
    14881 aaacacacta actcagcagg ttaatcacaa attgaccgaa ggttttgaga aaacttcgtc
    14941 tacttttatt gacgtggtaa agcgattgac catcattgat gaggcacaaa agaaaatcac
    15001 tgaactctcc agtcatgttg tcagccttca ggacgtatta gtggacaaaa aagctcgcgg
    15061 tgcttttggt gaagttcaat tatcaacact catcagcaat atgcttccca gcgcaagtta
    15121 tcaaatgcaa tataccctca gtaatcaaaa acgggctgac tgtgttttgt tcttacccga
    15181 gcccactgga aatattgtca tagacgccaa atttcccctg gaaacttatc aacgattaat
    15241 caacagtgat gccttatcca cagatcgtaa gtcattacaa caacaatttc gccaagacat
    15301 tcaaaaacat attaaggaca tcgctgaaaa atatatcgtg cccaatgaaa caacagatgg
    15361 tgccatgatg tttattcctg cagaatctat atttgctgag attcatgcaa actatcctga
    15421 tttaattgcc atgtctcaaa agctaaaagt ctggctggta tcgcctagca cattaatggc
    15481 tgtactgact acagcaaggg cagtacttaa agatgatgca actcgcaaac aagtacatat
    15541 cattcaaaaa catcttcaag ccctggccaa tgattttcaa cgttttgaaa agagaatgga
    15601 taaattatcc aaacacattg acttggcaca tcaggacgtt aatgaagtca atacatcagc
    15661 caaaaagatc acttccagat tccaaaaaat tgaatcggtc gatatcgaat tggatgaaca
    15721 cgaagtaact caattagaat tggtcgagtc tgaatccatc taaaataaaa tctttatggt
    15781 gcagaatacc taatttagca ttatactaag cggttttcag ttttaatcat taaattccaa
    15841 tgaatacaaa aacgctactt taccaaccca caaaaaataa acaaatatgg gggcaactct
    15901 atggtagcag tctggcactt gctttagctg aatattgcca acaaacaccc gggatcaaat
    15961 tacttattac gcaagacaac ctatgcgcca gtcagttgca ggatgaacta caattttttc
    16021 taaacccgaa ccaaaatacc caggagttat tatttttccc cgactgggaa accttgcctt
    16081 atgatcaatt ctctcctcat caagatatta tttcagaaag actctacacc ttaagcagag
    16141 ttcaacaaat taccaatgga atcattattt cttcagccag cactttaatg cacagacttt
    16201 ctccgccaga gtttttaaat caatacgctt taatgcttaa agaggggcaa aagcttgatc
    16261 tgaactcgtt caggaatcag ttgcaacaag caggatacca ttgtgttaat aaagtgcttg
    16321 aacatggtga atacgcacta cgtggatcta ttattgacgt gtatccgatg ggtagtggtt
    16381 tacctttccg tatcgagcta tttgatgatg aaattgaaag tctcagagaa tttgatccgg
    16441 aaacacaacg cacaatagaa aaaatcaatc aaatccattt gttacctgct cgtgaatttc
    16501 cattaaatga acaaagccaa ttacacttcc gacgtgcttt cagagagtct tttcctggaa
    16561 atcccagcca ttgcccaata tacgaagcaa ttagtaacag tcaatttcca tctggcatag
    16621 aatattatct gcccttgttt tttgataaaa ccgttacctt ttttgattat ctgccagcca
    16681 atgccaaaat ttgtttaatt gaagaaatcc aggagaatgc tgagagattc tggcgagaat
    16741 taaatgaacg ctatgaacaa cggcgtcatg atgtcagccg tcctatttta accccagata
    16801 actgctttat taatcccacc gagttattaa ccaaagctaa caattatgaa caagtacgcc
    16861 tgtatcataa gcctaccgaa aaaaaaggag ccttaaattt caatattgcc aaagctccac
    16921 aactacccat tgacaggaaa aatattgagc cactccgcca attaagtgag tattgttcag
    16981 atccaactcg acgctatctt attgttgttg aaagcgctgg gcgtcgcgaa gtattactgg
    17041 atctgcttaa atcaagccat atcattccaa gaacacaaca ctcgtggttg gacttcattc
    17101 atgatgaatc ccccatcaat ataactactg gcccattgat ttatggctgt gaattaaccg
    17161 acaaacaaat tgtgatcatc gttgagtcgc aattatttgg tgagcaaagc acacctcaac
    17221 gccgaagcac tcaaaaatca gttgatcccg acttaatcat acgagatatg gccgaacttc
    17281 gattgggcgc tcctgttgta catcttcaat ttggagtagg tcgctatcaa ggtttgcaat
    17341 acatcgactc caatggtacc cccagtgagt tcctcgtcct tgcttatgca ggggatgaca
    17401 aaatctatgt cccagtcacc tctctgcata tgataagccg gtatactggt gtggacagtg
    17461 aacacgcccc tttgcataaa ctgggttctg atcaatggca aaaggaaaaa aagaaagcgg
    17521 ccgagaagat tcatgatgtt gcaattgaat tattggatct ctatgctaaa agagaagccc
    17581 agccagggca ccaatacgat ttcaatcaat ccgaatatat aaaattcgcc agtggcttcc
    17641 cctttacaga aacaccagat caacttcagg ctattgaaca aattattagg gacatgcaat
    17701 caccaagacc gatggatcgc ctaatctgtg gtgatgtagg atttggcaaa acagaagtcg
    17761 ccatgcgtgc tgcatttgta gcagtacaaa atggaaaaca agtctgtatc cttgctccaa
    17821 ctaccttgct tgcaggacaa cattttgagt catttcgcga tagatttgca gagtttcctg
    17881 ttaatattga attattatcc cgctttcgca gtaataaaga gtccgaagca gtccttgccg
    17941 cattgcaatc aggtaaagta gatattgtga ttggaaccca taaattattc caaaataata
    18001 tttctttcaa aaacctggga ttactgatta ttgatgaaga acatcgattt ggagtgaaac
    18061 aaaaggaaca tattaaatcg cttcgtactc atgttgatat tttatccatg actgctactc
    18121 caattcccag aaccttgaac atggcaatgg cgggaatcag agatatttca cttattgcaa
    18181 caccgcccgc caagcgtttg gcgattaaaa cattttggca agaaaaaaat gatctcacca
    18241 tacgagaagc gatattgcgc gaaatattaa ggggagggca agtattttac ctgcacaata
    18301 atgtacaaac tatagaaaga gtttgccagg atctggaagc tttagtgccc gaagcaaaaa
    18361 ttcaaagcgc acatgggcaa atgcgagaaa ggcaattaga gcgcatcatg tctgattttt
    18421 atcatcaccg atttaatgtc ctggtttgca caaccatcat tgaaacaggg atcgatattc
    18481 caacggccaa taccattatt atcgatcgtg ccgataaatt tggattagcc cagctgcatc
    18541 aattacgtgg tcgtgttgga cgctctcatc atcaagccta cgcttactta ttaactccca
    18601 atgaaaaact gttaacgcca gatgcagtga aacgtctcga agccattgtg tctttagaag
    18661 atttgggagc aggattcacc ttggcaacac acgacctgga aattcgtgga gcaggtgaac
    18721 tccttggtga agaacaaagt ggtaatatgc atgccatagg atttaattta tttatggaaa
    18781 tgcttgaccg tgccgttaac gatttaaagg caggcaaaac tcctgaattg tcagcaccaa
    18841 tgcatcaagg acctgaaatt gacttgagaa ttagcgcgat tatccctgaa gattacatag
    18901 gtgatataca caatagactc atcatgtata aaagaattgc taatgcaaaa actacccaac
    18961 aacttagaga actacaaata gaactgatag accggtttgg tttactccca caaccagtca
    19021 aacatctctt tttgattact gaattaaagt taaaagctgc acagctcggt attcaaaaaa
    19081 tcagtgccag tgctcaacaa ggtaaattgg attttagtga aaaaccatcg attgaccctg
    19141 gaacactgat tagcttaata caagtacatg ccaaacgcta tcaaatggaa ggaccacaac
    19201 gtctgcgttt tactttagac agcacgtcag cagaggaacg aatatttgaa atcagttctt
    19261 tattaaataa actgtctcct cattcccaaa cgattgactg agatttctgc agtgcctttt
    19321 ggattaactc ggcataatct ttttcaacta cccttctctt tactttcagt gtaggtgtta
    19381 atttatcatt ttcagttgtc caggcatctt tcagcacaag gatgtgactg actttttcgt
    19441 atttaaccaa tttggaattg acttgcttta aggtttcttg caatgcgtga gtgatctctt
    19501 cttttggcat acccaaacgc actccttcat tcaaggtaac taacaaaacg ttactaggta
    19561 actctctacc taccaggcac agttgttcta gcatagggtt cactgaaaac agtttttcta
    19621 tcggtgaagg ggcaatgaac tctcctgtct gatttttaaa gttctcagaa agtcttccta
    19681 atattttaac tctgtcttca ttatcaacat caacaacatc tcctgttcgc aaccaaccct
    19741 cttctgtaaa ggcattttta gtcgcttgtt catttttata ataacctgac attagacaag
    19801 ggcacttcat tagcaattca ttctcttcgc ctacttttat ctcaacctcc agcctcggag
    19861 taccaacgta accacgtttt ctctcattta acatcgataa ggtagcataa gccaaattct
    19921 cagattgacc atatccttcc tgaatataaa ttcccaattt ttcaaaaaaa tcaataatgg
    19981 atattggcag atgagatgcc ccgctaaaac aattagtaca ctcgcttaat cctaaactgt
    20041 gaatgatctt tctttttatc agactggata taagaggaat ttttaataaa aaattcaatt
    20101 tggctggtgg cagtttttgt tcaattttct gcttaaatac tccccaaatg cgtggaactg
    20161 cagtgaaaaa ggtaggactc acttccctta agttctctgc aaacttctcc agactttcaa
    20221 caaaagatac cgatgcatta atagcaacac tgcctaattg gatggcagag cgttcataga
    20281 catgagccag gggaagataa gatattaatt tataatgttc caactctctg attctgagca
    20341 agtccttggg gaaaactgtc aagtaattgg ctatggaccg attggtatac atagcccctt
    20401 tgggtgcccc cgaagtcccg gatgaataaa taatggtata caacgcatct ggctctggtt
    20461 ccaccaggtt aatcataggg tcacttttca aaacatcaga ccattcgtaa tcaacttgca
    20521 aatctttatg gtaatcaaaa ccaattgtag gataactctt agggataaat tgtctgaccc
    20581 gctgatgatc atctaatttt ccaacaaaaa ccagtttcac atccccatgc tctaaaacat
    20641 aatgcgcact ttcttcattt tgattagcaa atagcggaac attgaccatt ccagcaatat
    20701 gaattccaaa atcagcgata aaccattccg cgcaattctt ggagataatg gaaacatgat
    20761 cgcctttttt taaccccaga tggtgtaaaa aaccagctac ttttctggcc tgcagcatca
    20821 cagacgacca ggtgtattca tgccaaactc cattttttgg ctgtcttaaa tacacacggt
    20881 ctgccgaatt tttttcattt ttcaacagta agtcaaataa tgagttggaa ttggattctt
    20941 tttgcatggc tcttccttaa gcttgtttaa ttactattct agaccaacta gttgtaaaat
    21001 attatttttt tctcgcaaaa cacaattttc accttcatga atcgcctctt ttgctttatg
    21061 aaaatcataa agcatgatgt ttcctaattg aggaaccaaa gtcaaatcag caggatcgcc
    21121 tgccgctctt gctcgggtta tccggtcctg cattattttg atgcttctgg tcaaaacatc
    21181 ataatatcct ggcccatcac tgattcccat tattcttttt atcattttat caaattggcc
    21241 tgagagaact gaaaaaaacc ccgggtgttc tgtcgtgctc ggaggtactc ttagcaagtc
    21301 atgatttaaa ttgacagcga ttacaacatc tgcccccata gctcgacaca atgtaatagg
    21361 aactggatta accaagccac catcgactaa ccagcaatcc ttataacgct gcggggtaaa
    21421 taaccctggt aaagacatgg atgaacgaat agcaagctca agagaccctt tacttagcca
    21481 tatctcacgt ccagtactca aatcagtagc aactgaacta aaaggtagcg agagctcctc
    21541 aatatttttt tcaagaccaa aatccttgaa aaaggtcatc aacttactac cagagagaac
    21601 cccgccacct aaaaaattca catcaatgag tttagccatt tcccttttat taagctttaa
    21661 cacccattgt tcaaacagat ccagggtgcc acaagcataa atggcgccaa ccaatgcacc
    21721 tatggaacat ccagcgacac aatcaatcct tacccccaat cgtttgagtg attgaattac
    21781 cccgatatga gcccagcctc gcgctgatcc actacctaaa gccaaaccga ttttagggta
    21841 ttgatttgtc aagaataact cctggaaata gttgggtaca cactcgttaa atcaatcaat
    21901 ttcatgtttt ttaacaccgg atgtcactaa atccaaaaac caaatgtagc gcaatgctaa
    21961 actgacattt tcttacttgg ttccgccaac cgtcaaggca tcaattttca aagtgggttg
    22021 tcccacgcca acgggaacag attgtccttc ttttccacat acaccaatcc ctctatccaa
    22081 tgccaaatca ttaccaatca tacttatttt tttcatcaca tcagggccat tgccaattaa
    22141 agtggctcct ttcacaggtt ttgtaatctt gccattctct attaaataag cctcactcgc
    22201 agaaaataca aactggccag aagtaatatc aacttgtccg cctccaaaat tcaccgcata
    22261 caatcctttt tttacagtgc taataatttc ctgagggtcg tactgtcctg acagcatata
    22321 agtgttggtc attctcggca ttgggacatg agcataggat tcacgacgac aattacctgt
    22381 tgattgcatt cccataagct tggcattcaa cttgtcctgc atgtaattca ctaaaattcc
    22441 attatcaatt aaagtcgtgc attgcgatgg cgtcccttcg tcgtctatag tcagagagcc
    22501 acgtcgattc tctaaagtgc catcgtctac aactgtaacg cctgcagcag caacctgctg
    22561 acccagtctc cctgaaaaag cagataatcc cttgcgatta aaatcaccct ccaaaccatg
    22621 tccaacggct tcatgaagaa gtacccctgg ccatcctggg cccaaaacaa ctggcatggt
    22681 tccagcagga gctggttctg ccttcaaatt ggttaatgct tcccgtaccg cttcacgcgc
    22741 ataactcaag gcgttttctt tctcggtaaa ataggaataa gctactcgtc caccaccgcc
    22801 tgatctcgcc gattctcttc taccatgact gtcttcaaca ataacactga cattaatgct
    22861 aaccaatggc ctcacatcag ccatcatctg cccatccata ttggcaacca ttactacttc
    22921 atagcaacca gttaaagtgg cattaacctg tatcactcga ggatcaagac tacgggcctc
    22981 tttatcaatg gcttccaata aagcgatttt ctcctgtttg ctcattcctt ccaaaggatt
    23041 taaactctca taccgattta cagaagcctt taataccttg acggccggta ctgtttttga
    23101 accagaaagg gctatggagc gcgccgcatc ggcagcacga agcatggaag gcagcaatat
    23161 atcatcacaa taagcaaagc cggttttatc accgcttaca gccctaatcc ctaccccctt
    23221 atccagagaa aaattaccgc ttttaacctc tgaatcttcc aaataccagg attcataact
    23281 acaactttgg aaataaagat cagcatcatc gacgttccga ttaaacatgg tcttgaataa
    23341 tttttctatg ccggcttcat ccagggaagc tggtgtcaat aaaatatttt ttgctatcgc
    23401 aagagatttt gtcatttata taccttttac attaaaacat gatgttcaac acacggaaat
    23461 tgtcttctca actgtgacaa tctctctaaa tcaatatcag ccagaattac tccctgccct
    23521 tcttcttttt gagccaacac tttaccccat ggttccacca ccatactatg cccataagta
    23581 tgacgcccgt tttcatgagt cccaccctga ttaggagcta agacataaca taaattttcg
    23641 attgctcttg ctctgagtaa gacctcccaa tgcgcagctc cagttaccgc agtaaaagca
    23701 gacggaactg aaagtaattg tgcgcctctt tgggttaaat attggtacaa ttccggaaaa
    23761 cgcaagtcat aacagatggt taacccaatt ttccccactg gcgtatccac tagcgctata
    23821 tcttttcctg cctctatagt taacgattcc tggtgttttt cctgctcgga aactatgaca
    23881 tcaaataaat gaattttatc atagcgagcg acatttaggc ctttatcatc ataaactata
    23941 caacttgctc ttacctttga acccatactt ttcaacggga ttgttcctgc tattatccat
    24001 acccgcaaat ctctcgctaa ctcacttatt ctttgttgta taggtccttg accataatat
    24061 tcggcgatgt gcaacttctc tcgttcattc attcccataa aagcaaaatt ctcaggtagc
    24121 acaaccaaac tggcttcctg ttccctggct tgaattaaat attgttcagt gagttgcaaa
    24181 ttatctgcta ttttggcaga tgataccatt tgcacaagcg ctactcgatt catccttttt
    24241 cctaaatgac attcatattt ttcatcttac tatatcgttc catataagcc caaaacaaaa
    24301 ttgacacgaa aaccttttcg ccttaataat aaagtttcta ttggcctata tgtaaatttt
    24361 attacaaaat tttcaagtga tacgacttga aaaataggaa atttgaccat agatatgcta
    24421 cactgataat aaataactgg agttctgagc aatgaaccga aatgatatca ccatacttag
    24481 tcaacaaaga gaatctgtgc tcgcaaccaa taaggtcctg cgaaatacgt atttactgct
    24541 aagtttaacc ttcatattta gcgcattaac tgcctacgta gcctttatta gtggagctcg
    24601 cccaatgaat ccactattaa tgatagttgg cgtttatggt ttgatgtttc taactcaagc
    24661 tttaagaaat agcgtttggg gtttggtaag cgtctttgct tttacaggat ttttaggata
    24721 taccattggc ccattactca attattacat cacaggtttt tcaaatggcc cacaaatagt
    24781 cgctactgct ttaggtggta ctggaatgat ctttttcgct ctatcaggat atgccctgac
    24841 aactaaaaaa gattttagct acctgggtgg atttcttttt gttggcgtta tggttgcctt
    24901 attggcgatg atagcgggaa tatttataca aataccagct ttgcaattag tgatttctgc
    24961 tgcttttgta ttaatttcat ctggattgat tctattacaa accagcgcca taattcatga
    25021 aggagaaacc aattacataa tggccactat tggtttgttt gtttccattt ataacttatt
    25081 tgttagcctg ctgaacctgt taagcgcatt ctcaggtcgc gaataattta atatcgtctc
    25141 ctctacctga gtcccggctg tatgccggga ttttttatgg atcaattgtt aagggaatat
    25201 cagagagtat ctcaaaaagt aattaactga aacaactgcc ccctttcaat ttttgagcag
    25261 ttatctgaag aataattaca ttgcaaaatc cagaggaatt attaatcaat gtgaaattaa
    25321 ttttgtttcc aactagcctt ggctttaaaa agttctggca aatcttccgg ccttttcttc
    25381 acttcctggc tcagagtaaa aagctcacga tccttttgat aatagactcc caaaggcact
    25441 gggtaatcag ggaacgtcaa tcgagctaaa cgcatggcgc taattaagct tttcgcatca
    25501 tgcttataaa gcgctttttc atcgactgaa atttgtacaa attgctcatc atccaactgc
    25561 agcgcttttt ctttttgagc gccaaatacg agtggctgac cgtcttccaa tattattgtg
    25621 ttttcagccc ggttattttt aactgcaaaa tcatcaaaag caccatgatt aaaaatattg
    25681 caatcttgat atatttcgac aaaagagcat cccttatgtt cataagcttt tttaagcaca
    25741 gaagccaaat ggacaggatc tttatcaact gctctggcta caaaacttgc tccggaagcc
    25801 aaagcgatca tgattggatt aattggctca ctagtcactc ctttggggga tgttttagtt
    25861 acttgccctt tctgagacgt tggagaaaat tgccccttgg ttaatccgta aacctgatta
    25921 ttaaacagaa gtatattgac attgacattg cgccttaaaa tatgcagtaa atgattgccg
    25981 ccgatactta gcgcatcccc atctccggtg ataacccaga cacataaatc ctctcgcatt
    26041 gcctttaagc cagttgctac tgctgtagcc ctgccatgga tagtatgaaa tccataagtg
    26101 ttcatataat aaggaagccg tccggcacac cctatgcctg agacaaagac gtgttgctca
    26161 ggaggcaaac ctaattcagg aagtactctt tgcaaagcag ccaaaatggc ataatcacca
    26221 catccaggac accagcgaac ttctgtttca ttcacaaaat cttcacgctt ataattgctg
    26281 ttcatagttg gcttccgctt taatggcact taccaaatga ttaacactga aaggttgacc
    26341 attgcattgg ctaattgatt gcgcatccac cagataaact gctctgatga gttgacataa
    26401 ttggccagaa ttcaattcag caaccaatac cttgtcatat tttttcaata aagaacccaa
    26461 atcatcaggt aatgggttaa gatggcgcag atgcagatag gcaaccggta atccctgttc
    26521 aagacattga tgtacagcag actttaaact accaaaagta ctaccccagc ctataacaag
    26581 cgtgggagcc gtcatatcac cctcaagaac caaagcagga taatctcttg caataccctt
    26641 tattttctcg gcacgtgtaa tcaccatttt ttgatgattc tcagcatcat aactaactcg
    26701 tccttcatcg ccttgctttt ccaagcctcc aatttgatga ataaacccag aagttccagg
    26761 aacattccag cttctactta aaaattcatc tcgatgatag ggtttggtaa aacgattaaa
    26821 ctccggtttt ggtatattga gagaatccaa agcaggaatt tcccagggct ctgccgcgtt
    26881 cgccaaataa gcatcgagta atacaataac tggagtcatg tacttgatgg ctatcctgaa
    26941 ggcttcaata attgtattaa aacaatcagc aggggattga gctgcaatca caggtaaagg
    27001 tgcctcgcca tggcgaccat acaaagcttg ttttaaatca ctctggctgg tttttgtggg
    27061 caaacctgtt gaagcccccg ctctttgcac atcaactagc accaagggca gctccgtcac
    27121 aacagccaag cccaggcttt cacattttaa atcaagccca ggccctgaag tacaggtcag
    27181 agccaaacga ccaccataag cagcccctat acaagaacaa atagcggcaa tttcatcctc
    27241 agcttgtatt aactgtacgc cgtaatcaga taagcgaaca cattcatgca aaatagcaga
    27301 agccggtgta attgggtaac ctgacaccaa cagaggaact tgtgtctgag tagctaatgt
    27361 agccagagct aaccctactg cctcaactcc agtgatttgc ctgtactcac ctgaatgtct
    27421 gttgacttcc cccagcatgt aatcccgacg ggaaagctcc agagtcatag cataattata
    27481 tcctgccaac aaagccaatt cattggcttt tgctatggcc tgatttgttt taaacttttt
    27541 agcgataaaa gcctggcaag tttctgtagg caaatcaaac aaccacaaaa ccaatcccaa
    27601 aatataaaaa ttcttggttt tgacagcctg tggttttgta aggttaatgg attcaactgc
    27661 ctgaatagtt tgtgtaatta atgggaaagc aacaatgtga taatgttctc gacaattatc
    27721 caggaaactc ttatcgatac ctgctttttg ccaatctctt tcttgaaaac tgtcttcatt
    27781 aatgatcagc aaaccacctt gattcaaatg ttgaagtgag ttttttaatg cagctggatt
    27841 taaagccact aaaacatcca atgactctcc ggcagtaaaa atagctcttt ccgccattgc
    27901 caactgaaat cctgagacac ccgcaactgt tccagcagga gcccggattt ctgccgggaa
    27961 atcaggcata gtacgcacat cacgtcctgt caatgccgcg ctaatggtta attgttcacc
    28021 taccaattgt actccgtcac cagaatcgcc agtgatgcgt acaacaatga catcagtcgt
    28081 tgacataaag tctcgcttcc tgcattaatt ggcgcatgtg tcttactgcc tcttttaatc
    28141 cacagaataa tgctctggca ataatggcat gtcctatgtt aagttcattt aactccctaa
    28201 tcgctgcaat cggtttaaca ttatgatagt gcaacccatg accggcattc actaccaagt
    28261 tcaaactggc agcaaattca gctgcctctt taattcgttg caattcataa tgctgctttt
    28321 cctctgaggt agcatcagca taacaccccg tgtgcaattc aataactggc gccccaacat
    28381 caaccgccgc tcttatttgc tctttatccg ggtcaataaa taaagaaacc tcacttccca
    28441 ttaattgtaa gcgacgtact gccttttcca ccacatttcg gtgagtcaaa atatccaatc
    28501 caccttctgt agttagttct tcccgctttt caggcactaa gcaagcatgc tccggcgata
    28561 tttcctcggc aaaatcaagc atggcttcag taaccgccag ctcaagattc atacgtgttt
    28621 gcagaacctg tttaatcaat ctgacatcac gcgcctgaat atgtcgtaag tcctctctca
    28681 tatgcaaagt aattccatct gccccagctt cttcagcatc cattgctgcc tggacaggat
    28741 caggataacg agttcctcgt gcttgtctta tggttgctat atgatcaata ttaacaccta
    28801 acaataactc tttattcatt ttgcaccaaa aaatataaaa tgcagagtat aatgcagttc
    28861 ttgacaaact gctattcacg ttaccttgtt tcaatcagtt taaacggcac tacctaaagc
    28921 atttaaccat actttacttt attcactacc actttaaaga aattataata ttgaagtcaa
    28981 actgatgcct gaataatagg cactgatcac atttccctga atcatcataa actgaacaaa
    29041 aagcttgata tgattttctt gtttaagtaa aatattaaac aattttaatt ataattgaac
    29101 atggtgtttg gtgttaacat tatcggattt ggaaaaaggc tatgataaaa atcttaatca
    29161 actctcatta tcctttttaa atcttagaga taatgacata ccactgctat gtgagtttct
    29221 tcaaaatcat cctgccataa ccagtcttga cctgtcacat aatgatatta cagccaatgg
    29281 tatgaagctt tttgttaata aaacatcggt ttcatccctt aacattagcc ataataatat
    29341 tggtccggag ggtgctcaat ggctatcaga agataaccac attactacgt tagatgtcag
    29401 ttttaacgag attggtgacg agggaattaa agctctagcc gctaacgcca aactgataac
    29461 cctttatgca ctttataata agataaccaa agtaggtgca ggctatcttg ctcaatccaa
    29521 tcttaagaaa atagatctat gcttcaatag tctggaagat gagggggtta tagcattagc
    29581 aagtaatatc aatataaaag agctgattgc aagcgcttgt gatgtctctg atattggagc
    29641 tattgaatta gctaaaaaca atcaactgac tttacttatt ttgggaaaaa acgcgataac
    29701 cgataaatca acactccatt ttgctaataa cacgtctttg agtacccttc atctgggtag
    29761 taatcaaatt acagcagcag gtaaaaaaat acttgaaacc aataccagga taacggacct
    29821 cgatttaatc ggcaatccaa ttgaagtgca tgaaacagat gaactaaatt attctaaaaa
    29881 atcggcaaac tcccatagtg tatttaagtt tttgcaaaaa tttacttttc tcagttgtat
    29941 gtcaccttgt caagaaaccg cagaaagcga taaaggctta aataaaaaac cgaattaatt
    30001 atgaaatcac aattatcata atcgagattc agtaaaaata acaacccaag aggtaatttt
    30061 ttattcatat ccataaaaat aaaagcacta tatttttaaa gaaatcatgg ttaagaaata
    30121 accgccacaa taaactcaaa tcagttatcg gagtaattta tggctattgc cccccaacag
    30181 atacaagaga gactgaagca agaacaatat caaaaatttg ttgttgctga tattgggaat
    30241 tttccacatt gccttgctca gactcctgaa ggcatagcta gcggacaaag ataccaaaaa
    30301 tacagtacaa actccttatc cagaacacca ccttttagtc aatggggagc tccccagctc
    30361 ttaaccccta aatcggctca ggaatatatt aaatttgccc agcaaagaaa taaaaaatca
    30421 agttttaaaa ttgatggcga ggcagttcgt gtctctgaat gcagcaattt tgcttaccac
    30481 gctgcaggag tattgttaga tgatccacaa ataagaactc agtatgatgt tgccgttata
    30541 ggaagtatgc acagtaacgg ccggtatcta cataatatta ccttactcgt tcctaaagga
    30601 agcagactcc ctcaaccccc acagcaatta accgctgaag tttttccaac tggtacgcta
    30661 atcgttgatc cctgggccgt tggcatggga caccctcctg aacaagcatt ggcgattccc
    30721 aaagaacaat ttgcttataa tcgcagcctt tttcctgcca cagtgaatta tcagtcagct
    30781 cttgacgaga gtcttacttc aaccagaaca ggccagctca ctccctatac tggtacacct
    30841 tcacaaactg gaagacaaga agtacgccca gaaagccaat tccctgatac aagaaggtct
    30901 gtttctgcaa cgttccccgg aaattttgct gcttatcatg aattatggcg aaatgctttt
    30961 caagaaatta tgaatgatcc tcgccatcag ctccacagaa atgatgtaga atacaaaaag
    31021 atacatgcca ttagaactgt tcttgatgat tatactaaag gaggcgacac ctggtgggcc
    31081 aaattcagac gaattttcac ttttcattgg aatagacacc atgtcaaagt agttgatgac
    31141 attgtcaaag agattgatgc tggtaattac actacctcac gagctttggt tgatagatta
    31201 gataatcttg ctatttcatt gggaagtaaa ctcaatccta aaggtacttt aaaagagcaa
    31261 attggtttta ttaaaattca ggagcaaaat cctgtggaag aaatcgaaaa tctatccaga
    31321 cctcacctaa cataataaac cctggacctg atgtgatagc atagaagcgc ttggcgatca
    31381 taccactatc tcaggccggt ccatacaagg accgggcctt gatttcccgt ccccccaaaa
    31441 gatgatcgat tgcctgacgc ataatgaatt tcgctgattt taaataagcg gactcgctta
    31501 aattatctgc tgctatagca agtaaatgct caccaggtat ttttttatca ccacctaaaa
    31561 taaagccctc cccagctaca aattggtaat gagaatcggg aacaattaac tctcctgttc
    31621 gcgcctcatg aagaaatgaa aatgtgtatc cacaagcctt aagcaaagcc cattcaaagc
    31681 gacgcagcaa agcttctatc gcctctcttt ctctagctaa tgctatgcca tttaaggtga
    31741 acaagtaagc atcaaataaa tccgggtcag ggaaatccgg gctcagcgta taatagagta
    31801 actcattgat gtacaggcct gaaaaaagag aatgtccttc aagatccagt ctggaagaag
    31861 tgctttcaat gtttcgtgtg taatattgat cataacgctc ttcgatacta acccacagag
    31921 gaataaaagc ttgaagtaat gactgtttct tgggagttcg ccctcccttg cacaagcaat
    31981 taattaatcc cagttccttg gtaaaaagct ttagtctggc actggtatct ccagaccatt
    32041 gcttatgaat tacccaagca ttcagcgatt tactggtcat atcccaattg cttcagtatt
    32101 cgctcatcat ctgaccagcc tgatttcact ttacaccaac attgtaaaaa aaccttttta
    32161 tctagcattt tttccatgtc caaacgggca ttagttgcca tttcttttaa tttttggcct
    32221 ttatcaccaa tgatcatgcg cttgtgatta tccttgtcta ctaaaatcaa ggcatgaata
    32281 cgaattaaat ttccctcatc cttaaatgat tcgatatcta ctgttacaga ataaggcaat
    32341 tcttgtccac aaaagcggaa aattttttca cggagcaatt cggcacataa aaattttata
    32401 gaccgatcgg tgaattgatc atcaggaaac aaatgtggtc cttctggcaa ataagtcttt
    32461 aatttgcctt ccaattcatc cacctgcaaa ccagttttcg ccgataaagg aataatggct
    32521 gcaaattgat atcgctgact tatttgctct atccaaggca aaagttgtgt tttatcagtt
    32581 atcttgtcaa ctttgttaac taccaggatg caaggaactt tagcttgttt gattaaattc
    32641 aatacatact catcttcgtc tttccaatgc gttccatcaa ctagaaaggc aatcacatcc
    32701 acgtcacgta gtacactaat cgctgtttta ttcatcatgc gattaattgc tttcgcatta
    32761 ccctgatgaa taccaggagt atctacataa acaaactgaa attcatcttc tgtacggatt
    32821 cctaaaatac tatggcgcgt ggtttgtggt tttctcgagg taatacttaa tttttgctgt
    32881 aaaatacgat tcaataaggt tgatttgcca acatttggcc tgccaactaa agcaatataa
    32941 ccacaataac tactcattaa tcttgattcc tgtttatttt taaaacattc taacagccat
    33001 gtcataaaac ttccagagat accaaatttt tcattaaata ggcttgctat ttctgttcca
    33061 attcgagtac cctgtgcatt aatggcatta aattaaacga ttttgtatta ttttagctta
    33121 aatgggcttt acttttcttc aaaaccaact taagcgataa aaaatcacta aattaatgac
    33181 agtatccttt taccatatat taaaccaaat aaatctttat gcctgatttt gaaactactg
    33241 atggccaaac tcaagttatt attccactca gagttactcc gcacacacac aaagcgatgc
    33301 caactatcac cggaagtatt ttcagtcacg aagccggtac agtattttat ggtaattata
    33361 atatggaata tctccttcct ggaggagaca gactacgctt aatagccaat cacaacaaga
    33421 aaacagttca gctgatgctc ttcactcata accctgaact gttaaaaatt cttccgggag
    33481 acagaccatc ctcgctagtt gctgatgtta taaatcacgt caatcgctat aaagttcaag
    33541 ctgaaagcta taaaaaaccc ctaagccatg agcaggaaga aaaaattaac ctggctaatg
    33601 cttgtatccg ttccttaaac catctattga aaaaagaaag acaattaaaa cctcatgatc
    33661 atgctggaca ggaggagtta agaagaaaaa tcatttccat tattgaacat tgccagaacg
    33721 acaatcgaat gatagccaat aatcctgtgg tttcagaagg aagttttggt tatatgctat
    33781 atgatgcgca caaaacagca cagcattatc ggtttaaccg agttttttct gtttctcgcc
    33841 aggatcaaat ggactttacc aaagtgacta aaacacaact tggaaaacca attactcctt
    33901 gttttgtttg ggatagtgaa atccatattg ggcacagtag ccgtgagctt gacgatgcgc
    33961 tcagggtaat ttgccagcaa taccaactct gccctgcgcc agcattaaac aacatcccgg
    34021 ccaatcgctt tgcaaaactg gaacgattca taagtaaact ttggcatgac ggtcacgatt
    34081 ggattaatta tctggcagtc acaacaaaac caagtcataa aactgaaata caaaaacgct
    34141 ctgatggagt atctattacc aaaataacgc cttactacaa actcaaagga ctgcctcaaa
    34201 taggttataa tagtctggct gcgatggtaa atcatctagt ggaaaactca gcccaacaga
    34261 cagaggtaaa cagtttaaaa gaggcgaaaa gattactaag ccacttacct aatggtaatt
    34321 gggttattgt gcctcaaaaa aatactgttc tgttaagact cgaaaataaa ctgattcatc
    34381 tcaaatattt tatacatgat caactctttt atcctttgcc ggatggagag gatttatata
    34441 ttttaagcca aatcagcaaa aggcatcttt acttgcctga aagaatcagc ctccgtttca
    34501 aagcttttat ttcccgtata cctgattttt ttaaaggttt ttacaaaagc atgagccaat
    34561 tcatcataca tgacctgcat gaagaatttt ttaatcatgt gcatgcaact catgccccta
    34621 aacaacatct ttcagaaaga gatcagcgtc caaaatccaa aaaaattaaa agaacctctc
    34681 tgcatgaagc gctccaaaat cagggacttc tcgcaaatgg acaaactctt gaagagttca
    34741 ttagtgatca gatcaacaac agcccctatg tcattgcaag agcgaatcat ccccctactg
    34801 tgcacaccta tgaaaatcct ttgcacagag ctttgggcgt agtacgccat ttagctggat
    34861 tttttataga caccagcgag cgtaacccca tgattggaac tcttgccatg gcagcttatg
    34921 tctatggcgc aggtgctgta ctcgcaccca atgttctcac tgacatttta aaaaagctgc
    34981 acttaagtgg cttgatttat ggaattgaac ctgttcaaaa attagctcgc ctgatgaatc
    35041 atggcaagat ttcagaagct atctctgctt ctgcaactct ctggcaaggt atggtagtga
    35101 gcggtaattt agataagttc tttgtcgatg ctgtctctgt cttgaaagaa gacccagcag
    35161 aaattgctat tattgtttct ttagcgctta gccttggtta tggtttaaca aaagcgattc
    35221 cttcactgca acatgaaatg ggtgactttc cttatgccaa ttatgctgct ctcggaggta
    35281 aaggaggtgc cgctatctat gataccatta tgcacccggg tgatgactgg ttccttggga
    35341 cttgcaagtg gttttgcaag aatattataa atattggaaa attattggtt gcaccttttt
    35401 ttgaaggttt ttactatggt ttttttgatg gatttataaa tggctggaaa aaaagtggca
    35461 aattgataaa acaactgggt aaacaaactt tagccgcaag ttttgatctt attttagcta
    35521 ttttaaccat ccccttactt gaggctagtt cgcttttaat acacgtccct tttagaggaa
    35581 ttaccaattt tttccgcaaa ttactgtctg tattaggtaa cttcagtgtg attgggaaat
    35641 tactcatcga tatagcagag cgtccatcaa caaataactt catatctgaa tttacgcttt
    35701 cccctcttta tggatttact tccccctttg gccatttttc agataatcca gtcacaaaca
    35761 taggcattaa tgtcataagg ctactttttt tacccctcct tcagctgatt aaaaatattt
    35821 tgattctccc tctgatagac atactttcac taacaataag agtaaattta tctattatta
    35881 atcctatttc ccgacttctt gcctattcta ccggtactat cctttatcac ttcggacaga
    35941 tttgggatca ttcagcagga gttttgttct caacaagcgc gtatggggtg actatattat
    36001 gtaaccacat agacagcaaa gcgggtgaat taaagcaata tttgctttcc ttaatagaaa
    36061 taaatcgtgg aaaagtatat caatgggcat tccatgatga ggatatactg acacataaaa
    36121 tagtgcagga taatcaatac tatttcaatg atcccagacg ttgcgaatta attcctcact
    36181 cagacagtca ctgcctgtta cgtaatttat tattggacga aaagagctcc accaataaag
    36241 tcaaagataa tcagaaatcc caacaactgc attttccagg tctgtttcat ggtgccacac
    36301 aaacatcaga cacaatgcct caagaacata cactgactat ctaaacactt ccaaatctaa
    36361 ttgcattgat ttcatggttt ttatttgcaa attatgctac ttgcttctaa gatgaaatac
    36421 taatattcag caaaaaaaga ctccaatcat gcatgagttg caaactcaag atcgcacgat
    36481 agtaaatggc ataatcctgt taaaacatga ataaactgat gctttattgg tcaacattgt
    36541 gtataatata agataactgg gggcgattga ttaaggatcc tatttatggc aaaagataac
    36601 aaatcacatc aagttaaaac atcagaaggg agtcttgaat ccgtaaaaac caaggaaaag
    36661 gaacctgttg tagaaaaaat gcgtgttgag gatagtaaaa aagaagataa gctatcaatg
    36721 cccaccacta aaaaagaatc tcaacccaat gagccagcca agccctttaa aacttctttt
    36781 gagaaatgga tagaatccag cttgcttgat ccccaggcta aggaagacaa aggttccacc
    36841 attaatctgg gccgagaagg gttaaaaaac gcatcccagg tcaaaaaatt tcttttatct
    36901 ccggcgggta aagacgtaat agctgagtta ggtgcccaaa tggctctgca aagaaacatc
    36961 aatctacaaa atcaacaaga cagaatggag catgagctct ttaaacgccg attaatggcc
    37021 gctttgtttt tgtggtattt atccaaaaaa tcccatgctg cggaaaaagt caaggaaatt
    37081 attcgagaat acaatgaaaa agccataaaa aatgctgaaa aagcaagcaa gccaagccaa
    37141 cagtccactt catccacttc acaagctgat aaagaaattc aaaaaatgct ggatgaatat
    37201 gaacaagcta ttaaacgtgc tcaagaaaat attaagaaag gcgaagaatt agaaaaaaaa
    37261 ctggataagc tggaacgtca aggcaaagat cttgaagaca agtacaaaac ctatgaagaa
    37321 aatttggaag gatttgaaaa acttctcact gactctgaag agttatcact atccgaaatt
    37381 aatgaaaaaa tgaaagcctt cagcaaagac agtgagaaac tcacgcaact gatggaaaag
    37441 cataaaggcg atgagaaaac cgttcaatcc ttacagcgtg agcatcatga cataaaagca
    37501 aaactggcta acctgcaagt tttacatgac gctcatacag gtaaaaaatc ctatgttaac
    37561 gaaaaaggaa acccggtcag ctcattaaaa gacgcgcacc tggctattaa taaagaccag
    37621 gaagtagtag aacataaagg acaattctat ctcctgcaaa aaggccaatg ggatgccatt
    37681 aaaaatgatc ccgcagcatt agaaaaagcc caaaaagact atagccaatc taaacatgat
    37741 cttgcaacca tcaagatgga agctctaatt cataagctta gtttggaaat ggaaaaacaa
    37801 ttggaaacaa taaatgatct tattatgagc acagatccaa aagaaaatga ggaagccacc
    37861 aaattgctgc ataaacataa tggattaaat ttaaaattgg caaatctaca agacatgctt
    37921 gcagtacata gaaaagaaaa atcctttttt aatgagaaag gagagaaagt aacatcatta
    37981 aatgatgcgc attacgtgat aggaaaagat caacaactgt ttaatttagg cggtaaattc
    38041 tatcctatcc ataaagaaca aaaaatactt gaaaaagacg gcaaatttta tcttttaaaa
    38101 caaggtgaag actgggaatc tatcaaagat agcccggaga aacaaaaaaa agctgagcat
    38161 gattttcata aacttcagta tgaaacacca atgacagtca agaaattagt acatcataat
    38221 aaaggtttag aaacaaccat tcataaagaa cgaattgaag agacgaaaca acaattagaa
    38281 gacaatggaa aagaaaaaat agaaatagcc aataacataa gtaaacttca atctacagtt
    38341 ggtgtggctt taaatcaact caaccaacca actttagata ctgaatcacc tgtcttaaca
    38401 ccttccggag gaagtacaac tgtttcaccg aaaccaaccc caagcttggc gtcagtgact
    38461 acttttttca agacgaagat agatgaaatg aaaaaaaatg aagatgttaa agtcaccctt
    38521 gatgatatta tggccctcag tattaaaatt gccgatccca aagcacgtac ttattttaag
    38581 aatgaagtgc ttaagtttgc tgaacaactt aaagaagtac cgcgctctgg agcaataccg
    38641 tttcaaacta tgcaacgtat gctgcaaact cttgagcaat atggggtaga tactacaaaa
    38701 ccaagtgtta cttctataag aagcaaaaca gatgaagtcc ttgagcaacg atttaaccct
    38761 actccgtctt tatctccatt caagacatca taagatagaa ttatctttca tctccctacc
    38821 ctggattatt atctgagaat cataattcag ggtgctctac ccccacctca gcatcaaaca
    38881 aattgttgtt tttcttatca atacccttgc caacaatcag caagtcttct aacattgaaa
    38941 gattattgca gaattttcgg aataaaacat gattattgaa aaaatcttag gtaatcaatc
    39001 tgtgatcata tcattggata tcgataattt tttatttgac agactcgatc aaattgcaaa
    39061 ttccgatttg gatttagtag agatcaactc gtccgatacc aatttattgg taaagattaa
    39121 aacccaatac ccatctctaa aaattggagc cggcggcata attgatactc aacaattaga
    39181 gcaatgctac caggcgggtg tacattttgc ctctagcccc ggctttttag cttctatagc
    39241 ccaaactgca agtgtttatt cgatgtgcta ttttccaagt gtagctacca tatcagaagc
    39301 tatggccgca gcgaatattg gatttaaaca agtgcgaccc taccccgcca atctgacatt
    39361 ttgtaatgct ctaagcaaat atttaccagg attaaatctg ttcccagctg aaattgactg
    39421 gaatgaagct gagcttttcc taaacttgcc agctgtagca gcagtaatta ttcataaccc
    39481 tgaccataag caactgaatg gattagccag gttagcacaa tcaattatct gaaaataaat
    39541 cgattctaat tggtaagcat tgttttccat taatgcatga caagctttgg tatagtattt
    39601 tgactttgaa aagggttaag atactaaacc acttcggagc tattatttcc gtaagagatc
    39661 agcctcctgc cgagaaagtc ttttttcgcc catcttgaac aaaaacagcc atctattctg
    39721 tgctttttca gtattatttg catctaacca gaacgcaaac cctactgtta ggggatagtc
    39781 aaacatgagg atactaactt atgacattat tttctaggca gtttgagtct attccctacg
    39841 aatcaataaa tccagaacta aaattgcagc tcctatacat atagcgctat cggccaagtt
    39901 gaacgtggca aaatggtggt gtttataata aacgtcaata aaatcaatca cataaccatg
    39961 aagacctctg tcaatcaagt tgccaatagc gcctcctaga ataagactta tcccaacaga
    40021 ttgaaatcgt gcctgttctg gagtacgtac taaccagatc agcaaaataa tactcattaa
    40081 aaaactaaac cctgcaaaaa accacctatg ccaatcgcct gcaccgctta aaaaactaaa
    40141 agcagcgcct gtgttaaaag ctaaagtgaa attcaacata ggcataactg gcatgggttt
    40201 atatggagtt aataacaccc caacccaata cttacttaac tgatcgcaaa taattactaa
    40261 aaaactaagt acaagccaag gccatttttt catatgtaaa gcctctcttc atgatatccg
    40321 ctgatatttc ctacacagcg caagcacagt tcaggatgct cttgattttg accaacatct
    40381 tcccgtctat gccaacatcg agcacatttt tcgtgaacgc tggctgtaac ctgtattgat
    40441 accccacaat cagtaacagc taatcctttg ggggattgac taattgggca agctttcgct
    40501 tctgatgtaa tgaataaaaa gcgtaactct tcgcctaaac gcgttaattt tggtaatacc
    40561 ttggcatcag cataaacagt tacctcagcc gccaatgcag aaccaatttc acctctttgc
    40621 ctggtttctt ctaaagcttt gtttacttca tcacgaacga tgtgcagctg ttcccaatcc
    40681 tccatgttca ctgcgtcaat agttggccag gcattatacc aatgctcaat aaaaatagat
    40741 tcgctattat tcccaggtat tgtttgccat atttcctcag ctgtaaaaga taagataggc
    40801 gccaaccaga tagtgaatgc tttgaccata tgatacatcg ctgtttggca agatcgcctc
    40861 gctatactgt cttttgccgt ggtgtattgc ctgtccttta tcaaatccaa ataaaaacta
    40921 cccatatcca ctgcgcagaa gttatggatt ttctggtaaa tcaaatggaa gtggtaattt
    40981 tcataggcag taattatttc ttcttgtaaa agttggcaac gctttagagc ccacctgtcc
    41041 agttctaata actccttggc ctcaatacaa tcactggctg gattaaagtc aaataaatta
    41101 gctaataaaa atcgcgctgt attacgaatt cttcgataag catccgcgtt acgcttaatg
    41161 atttcctcag aaatacttac ttcatgacga taatcagttg acgctaccca taatcttaaa
    41221 atatcggcac catgctgatt cactaattta tctaaagcaa cataatttcc tttagacttt
    41281 gatagttttt ttccctcggc atcaacagta tacccatgag tcaatacggt tttgtaggga
    41341 gcaaccccat acattgcaac tgcagttgtt aatgaggaat taaaccaacc tctgtgctgg
    41401 tcggatcctt caaaatagac atcagccgga aaatctaaat cattattatg ttttaaaacg
    41461 ctataatgtg atattccgga atctagccaa acgtccatgg tatctgtgat tttatcatag
    41521 tgtttggcat catcgccaag caactcggag ctatccagat caaaccaggc gtctatgcct
    41581 gatttctcaa tcatcatagc aaccctctct attaactcca aagtgtcagg atgtaattct
    41641 ctggtggttt tatgcacaaa caatggcatg ggtgttcccc aagatctctg ccttgatata
    41701 caccagtcag gcctattttc aaccatatta ctgatgcgcg ccttacccca gtcaggaacc
    41761 cagttaacct tatctatctc gttaattatt gcttgtcgca aattggattt atccattgag
    41821 ataaaccact ggggcgtagc cagaaatatc attggcgatt tatgacgcca acaatgcgga
    41881 taactgtgcc tgatagattc actagctaac aaaacattcc gctccgacaa aactgctaga
    41941 atagtctcat tcgctttcaa tacagaaatg cctgcaaata actctacatt ctcagcaaaa
    42001 catccattgg ccatgacagg atttattaat ggcaactgat agaactgacc taccaaatag
    42061 tcatcagggc catgagcagg tgcagtatgc acacaacccg taccagactc tgttgtcaca
    42121 tgctcagcaa gaactacggg aacctgacgc ttataaaatg ggtgctgcag tttaaaatgc
    42181 tcaaagactc gccccttagc ggagcccgat gttttgtaat ggctgattcc ataacgcgcc
    42241 ataaccgact caaccaaatc agtagccacg atataataag aattacctgc atcaatcaat
    42301 gcataatcaa tttcaggatg caaacatacc gcttcgttag caggcaatgt ccatggcgtt
    42361 gtagtccaaa taggcagaat taatggtttc accgctggct gagtttcaaa acaattaagg
    42421 aactcggatg gatttactgc tgagaacgcc acatcaatgg atggagaagt tttatcctca
    42481 taatccacct ctgcttcagc caacgcagat ccgcaatcta tacaccaatg aacgggttta
    42541 aagccttgtt gtaaatgacc atttttaatc attaaaccta aagctcgtac tatattggct
    42601 tcataatgat aatccatagt cacataaggg ttataccagt ctcccaagac tcccaatcgt
    42661 tgaaactcgt ctctttgtat atcaatttga cttgctgcgt attctcgaca tttggcacga
    42721 aattctcttg gactaatctt gctccctgct ttacctactt ttttttctac attcaattct
    42781 ataggtaagc catgacaatc ccaaccgggg acaaaggggg catcaaagcc actaaaggtt
    42841 tttgatttaa taataatatc tttgagaatt ttattgagcg catgtccaca gtggagatgt
    42901 ccgttggcat agggcggacc atcatgcaaa ataaagcgct tacttccatc acgagcttta
    42961 cgaattttct tataaattcc ctttgcttgc caatctgcca acatttcagg ttctcgcact
    43021 gacaaacttg ctttcatcgg aaatgaagtg tttggaaggt ttaaagtatc tttatattct
    43081 gccataggtg cctactctgc caaatcatta agtttttgat aattttaaat gcccttgttc
    43141 tgtttcatct ccacagcccg aagggagctt acaaaaaata ccccgattaa gggtaaatta
    43201 aacggtatta ctgattccat tcacaacatc attcgatgaa tcaatcgcac tatcaagcca
    43261 attctgtttt aaataggtct ttgccaatat gatgtcacta ttaatctgcg tgattaacgc
    43321 ttccacactt gagaatttaa cttcatctcg aagtttgtgc aagaaaatta cctgcactaa
    43381 ctgaccgtat agagacccgt gaaaatcaaa taaatggacc tccaaaatat ttttagttcc
    43441 agctactgtt ggccgttttc caatatttgc aaccccataa aagacattcg aattaatatg
    43501 gacttgtaca gcatatacac cttgcaaagg gatcgataaa cgatgaatgc ctaaattggc
    43561 tgtcggtatc ccccattggc gaccacgacc atctcctttg ataacacgtc cacacatgct
    43621 gtatggtctt cccaacaact tggctgcgaa acgcaaattc ccctgttgca acgcttctct
    43681 gattctcgtc gaactgattt tttcattttc tataagaaaa tcagattgca ctgtcacttc
    43741 acaggaataa ttagcaccta gtgttttgag taaattaaca tcaccttctc tgtttttacc
    43801 aaaacgaaaa tcctggccaa ccagaaggta gcgtattttc aatgcttcaa ataaataaaa
    43861 ctgagcaaaa tacaaagcgg gagtttgagc cagccttgca tcaaacttga tgcaatagat
    43921 gtaatccact tgacataagt ttaagacatc tattttttct ctcaaggtag aaagtcgtgc
    43981 tggtgctttc tctctgtgaa aatactcttt aggctgaggc tcaaacaata aaataactaa
    44041 aggcaagttc atttcatcag cctttgctct tagggcctta atcaaatgct gatggcctaa
    44101 atgcacgcca tcaaaattgc caatagtggc aactgcaccc ttatcaaata tagcaaaatg
    44161 agtagaactg cgtaacaatt tcatgaatcg ttttaatcag aagaataaaa aacacatagt
    44221 atactggttt tctgagatat tgagaaatct ataaaacatt atccgtacta ccaggtagta
    44281 cggaatttct taagttagag ggcgctctta gtggcttatt ttgggtctta caatcaagac
    44341 gtcgcattca gcaccgtgca agattgcatt agcggttgaa cctaataata aagaaaggcc
    44401 atgttttcca tgacttcctg tcacaattaa atcaatttta tgttcttttg ctacccttag
    44461 gatttcattt ttagttgaac catactctat aaaacgatgc ttttcatcta cacccagttt
    44521 ttctgccaat tcattcagct ctttttcagc ttgctcacga atagacattt ctacttctgc
    44581 aaatccagca aaacctgggt aagcataagc tggaataggc tctacaacat gaactaaaat
    44641 taaatcagca ccattctcgt cagcaatttt tttagctttc tcagctgctc gcaccccaac
    44701 ctcatcaaag tcagtagcaa acaataccct tttatacatc tttttctcct taaaattctg
    44761 atagctaagt taagattagc agatttaagc ctattttccc agaatcaatc aacaactaac
    44821 agctatatcc aagctgatta tcttagtcta accaatgttt gaaagtccct ccacagatcg
    44881 tatccatgga cttagtttgg ctaattcgcc tggtagtttt tttatattca gcagagcttg
    44941 attcctgctc tcatattcac cgatagttaa tatgtaccaa ctacctttcg cattagtaaa
    45001 atgtcttatt tttgcctgag ctaaccattt gttttggttc ttaaacctgt tgatatcctc
    45061 cactttatga ctggcagcca actgtatagt aaacagctta ctattaattt tcttgatgcc
    45121 tggttgctca ctggctaatt ttgtttgtga tgaatgggga ataggaggta attttgactg
    45181 actaactacc gcttgcccaa cctgttctga aatgttttta gctttcagtt ttggaatgac
    45241 aacgacctta tctacaacag ctactgtatt aagactatcc tcttcatcca taaaatcatc
    45301 taaaatttgt tttttgggta acccataatg cactagctgc ctcgttgaag aatcttccca
    45361 tgaagcaata tagcttacag attcttgttt agggacagct tgttgtgtgg gagtttttat
    45421 aaggtcattt atgggtggaa gtgttgttac agaagttaaa tctggcagtt tgagagtatt
    45481 atcaggtgct ttatagacat tatcaaaata aaaataggac aatcctgcaa caatagcgaa
    45541 gctcgcgact aaacttatt
//
LOCUS       DASLOE010000007        44004 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.007, whole genome shotgun sequence.
ACCESSION   DASLOE010000007 DASLOE010000000
VERSION     DASLOE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 44004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44004
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.007"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(61..570)
                     /locus_tag="U6309_000291"
     CDS             complement(61..570)
                     /locus_tag="U6309_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503753.1"
                     /translation="MKKWNSFLLLIYLSLFIGHAHAITSLLTNTSLLTNFAQLVKAVE
                     QGDDVKAIIRLDRCQITDPALQTQLVQNLDGASTRFNFTEYLHFRTRINDQLRDTVTT
                     VSSKIFEYSPGVFLSVSSRLNVFEDNTATLRVNFYNPALGTSVLIIDWLCDISNGNDE
                     NGLFLYNSP"
     gene            1115..1771
                     /locus_tag="U6309_000292"
     CDS             1115..1771
                     /locus_tag="U6309_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEN5503754.1"
                     /translation="MQNIQHLIDCILHIDVYLNAFVSTYGFWTYLALFAVIFCETGLI
                     VTPFLPGDSLLFAAGSIAAQPGNPMNIMLLFALLFMASILGNQVNFLVGRVIGPRVFS
                     AKRSWLLNPKHLHDTHAFYEKHGGKTIILARFIPIIRTFAPFIAGIGRMRSLHFTLFN
                     LISAILWIGSLLSLGYYLGSIPLIKENFSLVIYGIIAISILPPIIALISKKSTSSIKK
                     "
     gene            complement(1835..4069)
                     /gene="lem21"
                     /locus_tag="U6309_000293"
     CDS             complement(1835..4069)
                     /gene="lem21"
                     /locus_tag="U6309_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem21"
                     /protein_id="HEN5503755.1"
                     /translation="MAKTIKATGDGACLFNAVSIGLSVEILSGRLDSQLDTPGYQVLL
                     DEFAKHHPQFNPKSWKTLKEWLAYYNDTRDIELILAPVLFNLNQKYQDHLDEEILNEL
                     TNLVWKNKANIENGQAWFQLQNTGDLGEALFPKLENLDLNKDRVPLLDKLREILKDYK
                     LELTRENVKQFLTEKAKELLSALKKKISSDPHAFQRGYSCDELKGMTDALAISLVENR
                     EEDITDNRIKIRLENKEEHWNVLCNEEDSERFLDSTPSRLKMTSLEAYRGDKQVSAPT
                     SEQLDLIEEPGVFLRERTIDNPGLGNCAFYAFAIGLVNIIQEEAKYNRRTMFDRWVGL
                     DRSISGQYDEILKLNLEDPDKELLDRLQSSLRIVTYQYQIRELRNVCVFRNGNYNRLT
                     GNSNFVNFAALYYGDPLDTDSRFNPFADSVPILIKMANIDRDSVHPGHENDVLVPLFL
                     NLLYGDATNPADITLETEPKSDSPIITAMNNITQDFFWGTHLDLNYLAEAFEVNLHVL
                     RNNSPIQEFVDIPERHTLTLTNSNNTHWTTQITTARATTGMLLGRNPHLSGAKRTGEQ
                     QRVNEEFPGKELANDFMIPPFDEISNPHHKKEVETNKPISKGFFHARPVVSEEQRKLE
                     QLKRIVANATLAYTTYSESIWFCFFHYHGQTGRDRANNFCKTFSKITDYGQAKEALVH
                     YLNDSRNGNTHPHSYRTMLLHELQGNHDNKKDLQYLSRHFTSSLNKLASVLEVQLFAN
                     RYVP"
     gene            complement(4160..4858)
                     /locus_tag="U6309_000294"
     CDS             complement(4160..4858)
                     /locus_tag="U6309_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HEN5503756.1"
                     /translation="MNLGILLCDKVSEVFVADHGQYPEMFANLLRPADSTLEFTVFDA
                     EHGELPTDIHAVDAYLISGSRHGVNDDYPWIRKLEEFVRTLHASQKKLIGICFGHQLI
                     AKALGGKVIKSPKGWGVGMSQNQIYQFKEWMRPSLNCFNLLVSHQDQVIELPTGAEIL
                     AGSDFCPNYMMQIGSFFSVQGHPEFTKSYSRDLMVSREDSVNEIEFAKGMKSLELHED
                     DTIIAQWIVNFLKA"
     gene            complement(4988..6148)
                     /locus_tag="U6309_000295"
     CDS             complement(4988..6148)
                     /locus_tag="U6309_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEN5503757.1"
                     /translation="MKNQSLIVNWNYPTRILVGAGRINELAKACRDLGMNAPLLVTDP
                     GLASSLMVSKVIQQAQASGLRTGLFCQIKTNPTGDNVMNGVCAFKAGQHDGVIAFGGG
                     SALDAGKAIALMVGQDRPLWDFEDVGDNWTRVNVSAMAPVLAIPTTAGTGSEVGRASV
                     ITDAEKQVKKIIFHPKMLPAMVILDPELTVGLPKLLTAATGMDALSHCLEAYCSNYYH
                     PMAESIALEGIRLIKENLIQAYKDGSDLEARTHMLVASAMGATAFQRGLGAMHALAHP
                     LGAIYDVHHGRLNAVLMPYVLLANRSEIENKIERLANYLSIANGFDGFLDWIEQMRLE
                     LGIENTLSQLGIDHSQIDRLAKMATEDAAAASNPVLFTFEQYRELLSKAIGE"
     gene            complement(6278..7675)
                     /locus_tag="U6309_000296"
     CDS             complement(6278..7675)
                     /locus_tag="U6309_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEN5503758.1"
                     /translation="MIATLKTYSPVDNSLYVEREYANQEEIKTVLSKAQVAKKLWSLS
                     SLAERQEYCLAAVNEIVACKVDIAEEICWQMGRPIRYAAGEINGFEERARYMIAQAES
                     SLASVELPEKEGFTRYIKREPLGLVLVIAPWNYPYLTAVNAIIPALMAGNVVILKHSA
                     QTPLVAERFDQAFKKAGLPMGVFQYLHLTHEDTEQLMRSPAVNSVAFTGSVAGGERVE
                     QTLAGRFIPVNLELGGKDPAYIRADADLDHAVETVIDGAFFNSGQSCCGIERIYVHKE
                     IYEQFVNKAVNLVKQYKLGRPDDPETTLGPLVRASSADFVREQIKEAIAQGAIAHINT
                     QDFVMDRPGTPYLAPQILTDVNHRMRIMTEETFGPVVGIMSVDSDEEAIERMNDSDYG
                     LTAAVFTQDIHRGIAIGERLQTGTFFINRCDYLDPGLAWTGVKKSGRGCTLSAIGYEY
                     LTRPKSFHIRTKIET"
     gene            complement(7713..9086)
                     /locus_tag="U6309_000297"
     CDS             complement(7713..9086)
                     /locus_tag="U6309_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEN5503759.1"
                     /translation="MLNPREIRTIEDAKRIVEERQLTHVKVGLFDIDGVMLGKYMSRN
                     KFFSALEHGFAFCDVILGWDSKDKLYDNVKYTGWHTGYPDASVRIVPSTCRDIIFEEN
                     QLLFMAEFSQAAEAICPRGILRRVINKAAGMGYDVYAALEYEFFVFDETPDSVRAKGF
                     RNLKPVTPDNFGYSIIRNTVHAEFYHQILTMAEKMDFPIEGLHTETGPGVLEAAIAVD
                     DAEAAGDKAALFKTFMKILAQRNNKMATFMAKWSGDYPGQSGHIHLSLRDRDGGKSVF
                     YESGREYNMSKIQRHFLAGQQKLMPEFLAMLSPTVNSYSRLIPGYWAPTEATWGVENR
                     TTALRVIPGSDKSQRIEYRLGSADANPYLALAAAIGSGLYGIENELEPEAEVKGNSYE
                     QNHSSALALPRTLWESAQNLKNSSAAYELFGQEFVEHFAASREWEEREFRKHITDWEL
                     DRYFEII"
     gene            complement(9138..9926)
                     /locus_tag="U6309_000298"
     CDS             complement(9138..9926)
                     /locus_tag="U6309_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HEN5503760.1"
                     /translation="MKSLLTLQAQFQEFIYSGQGSIKESIVPTKLVSTETRLNIYKDG
                     YRLRLIECLAANYPALYAYLGTEEFEKLSARYIDSYPSAYRSIRWYGDRLPELIKKYY
                     AKSCYYLYELAELEWKMTLAFDAADDTVLNVEEMAAVPPESWAEMGFVLHSSVHRLDF
                     CWNVFSLWQALVNNLEIPELKDSPATTGWVLWRGQNYIIQYYSLSEEEAWALDALIQG
                     SSFGVICEGLCQWMPAEEVGMKAASYLKNWIQNGLLSQCRICSN"
     gene            complement(9923..10786)
                     /locus_tag="U6309_000299"
     CDS             complement(9923..10786)
                     /locus_tag="U6309_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEN5503761.1"
                     /translation="MEVSPNKSDQKLPYLGFGLGLRPNYYEEILTSKPDLDWFEILTE
                     NYLIPGGKPLYYLDKIREHYSIVMHGVSLSLGSTDPLNWDYLKQVQELASRIEPVWIS
                     DHLCWTGVHGLNTHDLLPVPYTTEAIQHIVSRIQEIQDFLKRPFLIENVSSYLTYKQS
                     EMSEWDFILEIVKQSGCYLLLDVNNVYVSSFNHHFDPMTYINSMPPGRVAQIHLAGHT
                     NHGDYIIDTHNAPVIEPVWDLYEATIQRLGPVSTMIERDDNMPDFSELLSEINHAKRL
                     AIQAAKERVAV"
     gene            complement(10862..11113)
                     /locus_tag="U6309_000300"
     CDS             complement(10862..11113)
                     /locus_tag="U6309_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503762.1"
                     /translation="MNKLQLTGAALAVGAASMFALAPAFAVDATTTTIHCKGVNACKG
                     QGSCKTADNACKGQNSCKGKGILEMSKEDCEKQGGTVAE"
     gene            complement(11544..12902)
                     /locus_tag="U6309_000301"
     CDS             complement(11544..12902)
                     /locus_tag="U6309_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN5503763.1"
                     /translation="MKLSFLGATGTVTGSKYLVESNNKKILVDCGLFQGLKELRLRNW
                     SPLPVSPESIDAVVLTHAHIDHSGYLPLLVKQGFKGRIYATAATTDLCSILLPDSGFL
                     HEEDARRANKYGYSKHHPALPLYTQEDANNTLKYFESVEYDTPYPLMEDCEISWHRAG
                     HILGSAFIQFQTKQIKLLFSGDIGRLNDPVMKPSELIKSTDYLVIESTYGNRLHDKTD
                     PLAFIANIVNTTCKRGGTVLIPAFAVGRAQTMLYYLYILKQKKLIPDVPVYLDSPMAI
                     DATELLLKHHNEHRLSAEECRAVCQIATYVHTPNESMAVDWGRESKIIISASGMMTGG
                     RVLHHLKVYAPDPASTILLTGFQAAGTRGEKMLNHEPSVKIHGDDYPVRAQVAMLTNA
                     SAHADYEEILVWLKHFEKAPKEVFITHGEPQAALGLKGHIEAELGWNCKIPNYLEKVN
                     LD"
     gene            complement(13025..13675)
                     /locus_tag="U6309_000302"
     CDS             complement(13025..13675)
                     /locus_tag="U6309_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503764.1"
                     /translation="MNYFIGLCFGLLNAVICYAEPNGSSLPKELIKVNKENNPKCVEF
                     VTYKGELYCSVVPLADSSGDSQVIHYEKQIVRFDDRPWKIAWGKKTDKVVTIEYIPVG
                     DDINNWKELITTQFMPGLINMTPAQFGNQFLHNMNKSVAKYIVHVIEEQPNLLIFEFK
                     IQEPANLQQDEILKITKGKDGIYVLHYAIKKSDMTKENRDKWVKNLKSSFIKASTP"
     gene            13792..14082
                     /locus_tag="U6309_000303"
     CDS             13792..14082
                     /locus_tag="U6309_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage holin"
                     /protein_id="HEN5503765.1"
                     /translation="MNGCKISPVGLGLSLGILWGLSLLVMGLIAYFYAYGRPFVEAVA
                     TLYLGYEPSILGSFIGGVIGFIDGFVTGFLIAWLYNCFSCCSCCRGKENKKE"
     gene            complement(14173..14484)
                     /locus_tag="U6309_000304"
     CDS             complement(14173..14484)
                     /locus_tag="U6309_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEN5503766.1"
                     /translation="MLKCLSSVIIVFLILFTAGCQTNTVSNMFIPSNPSGMTLAQSVK
                     DSLMESNDPVINQIHVESNQNVVILSGYVKKIRQSDIAEQIARQVQGVQSVENRIIVR
                     P"
     gene            14701..16488
                     /locus_tag="U6309_000305"
     CDS             14701..16488
                     /locus_tag="U6309_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN5503767.1"
                     /translation="MKKTTEKPINKTKEMPASSKKETLNPLEQTPSPEQSDPIFRFID
                     KLYQANLGKLTAGISPAALGTAYYSWFAQLLQSPGSMLRLAYYPLLHANDYLSNLFKY
                     DKPRDGKDVRFHTENWSYYPWRLWAEQFLQFEDWCLQASSKVPGIPLHVKRTVTFSTR
                     QILDALSPSNFVLTNPDLLQETIRSNGQNLIRGTELAFQDFVEKITGSPPAGVENFIP
                     GKQVAVTKGKVVYSNHLIELIQYTPQTEKVYKEPILILPAWIMKYYILDLLPENSLVN
                     WLVRQGHTVFIVSWRNPTKEDRNLGLDDYYKLGAMDAINAVSNAIPHTKIHLMGYCLG
                     GTLALLTAAAMAHDHDNRLKTLSLLAAQGDFIDAGELLLFITKSEVSFLKSMMWEQGY
                     LDTKQMSGTFQMLRSYDLIWSKMVQDYMHGTQRGMIPLLAWNADATRMPYKMHSEYLE
                     KLFLNNDFAEGRFILDGKPVVGENIRIPAFVVSTEKDHVAPWKSVYKTHLLINSDITF
                     VLTNGGHNAGIVSEPGHEGRYYRIRERKMDSTYLDPTNWLKKAELREGSWWIAWHDWL
                     VNHSSQKQVSAPKLDKNLPNAPGKYVLQK"
     gene            16500..17906
                     /locus_tag="U6309_000306"
     CDS             16500..17906
                     /locus_tag="U6309_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HEN5503768.1"
                     /translation="MNNEFLENVTYEELTIGRKASLNRTLTQDDINLFAAMSGDINPS
                     HVDPQFAKSDVFHGVVGHGMWSGALISTLLGTALPGPGTIYLEQDIQFITPVRIGDNL
                     TITIVVRDKHPGKSIVHFDCMGVNQKGETVIKGLAKVIAPTKKMKVPRTVLPLVQIHH
                     NDHFNKIIETCSNLPSIKTAVVHPVTANVLEAVYDSVKAGLITPVLIGPMAKIKCAAS
                     EAKIDLSNWEMIDTEHSDAAAFKAVELAASGKVDSIMKGALHTDELMSAVVPATSGLR
                     TKYRISHAYVMDVPTYHKPLIITDAAINIAPNAADKADICQNAINLWRILYGEETKPK
                     VAILAAVEMVNPKMQATVDAAVLCKMADRAQIINGILDGPLAFDNAINKQASKEKNII
                     SPVAGDADILLVPDIESGNMLAKQLTFLGHADAAGIVLGARVPIILASRADSLRTRLL
                     SCAVATKIAAARKAGKIK"
     gene            17903..19021
                     /locus_tag="U6309_000307"
     CDS             17903..19021
                     /locus_tag="U6309_000307"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEN5503769.1"
                     /translation="MRHSALLIINSGSSSVKFQVFSNTEKLDLLAKGKIINIGGEPSF
                     TAVNETSEHQRTLITLEKDMTYEQAIHLIMDWISQQQQNWEIHAVAHRVVHGGEYFTQ
                     SVLVTPQIISQLETLNSLAPLHQPHNLAAIKIIGRIKPGLPQIACFDTAFHAQHTPLF
                     TTCALPEYLRQQGIRRYGFHGLSYEWISLVLKKDYPDLANNRIIIAHLGNGASLCAIN
                     RGISIDTTMSLTALDGLPMGTRCGSLDPGAVIYMMRELNLPPDKVEEILYNESGLLGL
                     SGKTNNVLELQSSPDKNAQFALEYFCLKTAQFIGMMTISLGGVDGIVFTGGIGENSEP
                     IRANILKRIECLNPFSVIVIPANEEKMMAMHTLTALRT"
     gene            19061..19951
                     /locus_tag="U6309_000308"
     CDS             19061..19951
                     /locus_tag="U6309_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEN5503770.1"
                     /translation="MDKDYYKIMGVSQDASEKDIKMAYRKLARKYHPDISKEPDAEER
                     FKEMAEAYEVLKDPKKRSEYDQYLKYKEFNPQSDGFTGRRTQEQPFQEFHFDSDFFET
                     LFGHSRYQQQPMTGQNYHGKITISLEEAYHGAVKNLQVPVEQGTETKIQTLKVKIPAG
                     VKSGQQIRLAGQGGSGSGGGARGDLYLTVEVMKHPIFDVMENDIYLTLPITPWEAALG
                     ATVVIPTLAGKIDLKIPPSSQGGQKLRIKNRGLPGSPPGNQYVLLKIITPPAQTEEAK
                     ALYKKMAEVMPYNPRKTMGV"
     gene            19954..20268
                     /locus_tag="U6309_000309"
     CDS             19954..20268
                     /locus_tag="U6309_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEN5503771.1"
                     /translation="MSKDNLLIGMLIEETTTISFIEVCQKYHIPEELFIEMIEQGLFP
                     DQPSDPEKIALGQKELRRLESAFRLHRDLGINLPGVALALDLLEKIDQMHHELEILRK
                     HF"
     gene            complement(20265..20828)
                     /locus_tag="U6309_000310"
     CDS             complement(20265..20828)
                     /locus_tag="U6309_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEN5503772.1"
                     /translation="MQKICRFIFKLIGWKIVGELPNEKKYIVIVAPHTSNWDFVICLC
                     ARFVVGVKINFLAKHQLFFFPLSYFLRAVGGTPVDRSKKGNRVEKVVELFRQRDELKL
                     GLAPEGTRSPVTRWKEGFYHIARQAGIPIVMVGPDYSTKEIRIHEPFWTTGDIKKDFQ
                     YIIEFFRTIKGYRPKEIPDYNPKDSDA"
     gene            20946..21497
                     /locus_tag="U6309_000311"
     CDS             20946..21497
                     /locus_tag="U6309_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEN5503773.1"
                     /translation="MLYHDPLISEHTMKWVRTFIINYTICPFAKGPVNKGTLRIITSD
                     TLKKHQALKDLITEIQFLEANPAVETTLLIFARAFKDFFSYLDFISLAEQLIQNLNYE
                     GIYQLATFHPDYYFADTSPDDVANYTNRSPYPMIHLLREDSLDKAIAAYGDTHIIPEK
                     NITTMNELGLEKMKQLLASISIP"
     gene            complement(21530..22768)
                     /locus_tag="U6309_000312"
     CDS             complement(21530..22768)
                     /locus_tag="U6309_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN5503774.1"
                     /translation="MNSILFKNANVILRESTEIQKNFDVMVQNDLIYQVSQTPLQPLE
                     GMRVIDVKGKTLMPGLIDAHAHVTGLTLSPKNIFYSEAETFLAAATYLKNSLFYGFTT
                     LREAGGADFRIAQLLDNKSIPGPRLFYSGRALTQTGGGADFRKPNEQIDPCGHVSSFS
                     TMSVIADGVDEVRKAAREELRKGATQLKVFASGGVVFPSLSNPTLYEYSEEELSTIVE
                     EARARNTYVMAHAYSDESVRKCIKSGVRSIEHANFVSEPTVELMSESGVFYDPTFISL
                     AQRIESAEQNRLSEAIVANLKNTIEKGKKVYEYALKYKIPIAFGTDLWGPEAQRDQLR
                     ELEMRKELDSAANIIRSATVVNAELLMQKGKLGVISEGAYADLLVVDGNPLVNLNVLL
                     RPDENLKLIMKDGVIYKNEL"
     gene            22969..23529
                     /locus_tag="U6309_000313"
     CDS             22969..23529
                     /locus_tag="U6309_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503775.1"
                     /translation="MNTQDPALSLSNPRLLSAIYFGLLSVVGTILINAFLTSIGIEEI
                     IPVYQSVILGMVVASCTGAIFGEAIVHCKKPYKRKTFWLGFSMVIASLPVFDLGLVFL
                     MSEDHAKLFEVTTLTSMVLFYLVVLAYSYVLFGILLAVASGLAAMYLRGQLVYDILHT
                     QTRRQQKHKHALAKSKSEGHAHKVHR"
     gene            23507..26053
                     /locus_tag="U6309_000314"
     CDS             23507..26053
                     /locus_tag="U6309_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEN5503776.1"
                     /translation="MPIKFTDELKSDELYNLLSQGAVVITPNNRLSGTILQNYFTYCS
                     RKTITKPICLPFNTFLVNSFEQLKFQKPDLEHPILLNSAQCQHLWRKLIKSHPSIIYT
                     EGLLKTIMQAWEYCEQWQLTPEAPAFHYTPQTQQFQQWWQSFNKILKEISAIHQHQLI
                     PYLLDSNYQGLAKTVVWMCFDDFNPQQLSLQNYLNTQGVIQYGYDLKENQETPEVFSA
                     KDKKTEYQELMTWLEKKIQQGKKQIGVVVPNLEQESSVLQRILRDCFEPNLFNISLGK
                     PLSAFHLISHALIWLSLDHNLLNNHQAALLLQSPYLGKAKDEFLQRSDYLQESKLLQD
                     QSFPTKLFIEDIAYHTPKLAELLKNMNFYPEKTTLDEWIELFQERLNGIGFPGDYVLN
                     SEDYQCFNRFTMIFDEFRQLSLISSHLTKKEAFETLKQLIDNTIFQPQKSNSPIQILG
                     LLEASGCEFDSLWVMGLTDRCLPQQTRLSPFIPFELQRELGMPHSIPARELQYAKQIL
                     QRLQRGSMETVFSYPQFDDDTPNLPCSLITHYPPYLSRSADNKTNVQSCLVPLEENYE
                     VPVKPGESISGGTSLLSNQAKCPFKALAQHRLKANPVLKTTDGMDDKEKGQLIHKILE
                     LLWQRLENQEHLIKLKPAELERFIDEAIQGALNPIRQRHSQAFPDLIANVEYMRLKRI
                     ILSYLEWEKQRPPFKITGLEENHSINLAGLDFKVRVDRLDEVGNKQWVIDYKSTLPGG
                     KPWNEERPMEPQLLLYALLNEQINTLLLIQLKTGKIKLSGFSEEKWETKEINTLKEDE
                     NWEDYRQRWLEQLTHLAEEIKSGYCPPRPQNLTICQRCDFQNLCRFQAKQ"
     gene            26081..26923
                     /locus_tag="U6309_000315"
     CDS             26081..26923
                     /locus_tag="U6309_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN5503777.1"
                     /translation="MHHLILGYGYCGYYLAQELLRHHQQVTVVSRQLKKELELSKLQH
                     ISHDLEYPFHWSEPDSIIYYLIPPPSQGTCDTFLQQFLNQSSLNAKKIIYFGSSGVYG
                     NHQGAWVDELSACIIDSPRQQRRLDAEQQWLAYCQQHNKELSLLRIAGIYGPNRIPAD
                     AAKSKIPIIKPTEAPFTNHIFVKDLSLIAYLLGQKQTTSAVYNVADGNPTHMGELQQT
                     VANALGIESASYESFEQAWEKASPMKREFMRASKRLKIDLLKTALGEDIQLTCLYDAV
                     KQSL"
     gene            27075..27725
                     /locus_tag="U6309_000316"
     CDS             27075..27725
                     /locus_tag="U6309_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2271 family Dot/Icm T4SS effector"
                     /protein_id="HEN5503778.1"
                     /translation="MPSYNNARLLEKRLQNCDTQLNQFFNGQELSIKFLQQLNQIKYF
                     YNSAFSKTENEDDGLEVVEEYENFISLVSQVKSGQINADKAFETIKDTTESRQADVII
                     ANFFKVCELMFWATAAFFSYLACVSVGIPLTFCDPLLGIAVSLSTFLMTISTASKFLD
                     CFDEFQSLDKINEHDKNQKDTVRFFSKTSTSISSVRNEEAEVEESSQRLYPNLQAI"
     gene            complement(27900..28388)
                     /locus_tag="U6309_000317"
     CDS             complement(27900..28388)
                     /locus_tag="U6309_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2165 domain-containing protein"
                     /protein_id="HEN5503779.1"
                     /translation="MIIRFSKVLLVVAVSFFCLLTAFGNITDYSANFPAVVKVLTMSD
                     IFPNSTITYRAITSPALHYVAFIIIVILELLTAIFCGIGAWKLFKARNESASVFNHSK
                     NWAIGGLTLGFVTWQVIFMSIGGEWFGMWMSPMLNSALTTAFHIFITILAVMIYLVIK
                     DE"
     gene            complement(28533..29846)
                     /locus_tag="U6309_000318"
     CDS             complement(28533..29846)
                     /locus_tag="U6309_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN5503780.1"
                     /translation="MKILFLLLLIFLSVIPAQAKPVELVFWHAMAGHLGDEVRLLADD
                     FNKSQNQYRVKPIYKGNYTETLTNFAAAFRARQAPSIVQIFEVGTSIMLAPPGVIKPV
                     DLLMSEQGISLPKDDFIQSVREFYSRDGQLMAMPFNLSAPVLYYNADILAKVGYGKHN
                     FPQTWSAMEIMAEKIKKAGYDCTYTSAYPGWVLFESFLAIHGLPITQGNPARAAFHTP
                     QLMAHFQRLKRWHDLHYFRYGGRVDDATILFTSSVCPLFSQSSGAYNSLSALVPFHLG
                     VATMPLDTGASPIRHANVAGGAALWAVGGQTEEQYRGIARFFAFIAKPEVQKRWHEHT
                     GYLPLGLKGIYANIVQSSQHPALLLARTDLEDNLSEKPYKHMGPQNQIRGINDEVLEA
                     MFADLMSPEEALNEATIRANHLLLRFARNTQRERVVDDKVGKGLG"
     gene            complement(29843..30562)
                     /gene="ugpQ"
                     /locus_tag="U6309_000319"
     CDS             complement(29843..30562)
                     /gene="ugpQ"
                     /locus_tag="U6309_000319"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN5503781.1"
                     /translation="MILEKIIGHRGASGYAPENTMASFNKALSLGCRFIEFDVMCSLD
                     GEPFIIHDDNLKRTTNGTGEVGLVEADYLRGLDAGSWYSKQYKGEKIPHFTDVLKWLS
                     FSDVQANIEIKPYPGTVEQTTVSVLSYIHRHWPQNKELPLVSCFEWDALVLCRSIAPE
                     MPLGLLLHEWDKDWLQKAKQLECYSIHFNRKILTADRVKAVKDQGYVVCAYTVNRKRL
                     AKKLFDWGVDALFSDYPDLLK"
     gene            complement(30567..31262)
                     /locus_tag="U6309_000320"
     CDS             complement(30567..31262)
                     /locus_tag="U6309_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503782.1"
                     /translation="MRKIAWILGLMVLTPFVWADNETPDLVLDKIFFQVSAKKWVTTQ
                     TALLNVSINATLSNADLVKARADIMDSLNKIAKGEWHLVQFDRSQDSSGLEKLYVQAQ
                     ARVDQSALTNIYQNAKSVSKPGAKYEISGVEFKPSLEETQVVRAKIREQLYQQVNDEI
                     DRMNKAYPKQNYSVSNLVFFEGDNIPQQPRAYQAKEMNALAMAAAPAPLTVSNELILT
                     AMVEAASNRKQGD"
     gene            31950..33023
                     /locus_tag="U6309_000321"
     CDS             31950..33023
                     /locus_tag="U6309_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid dehydrogenase"
                     /protein_id="HEN5503783.1"
                     /translation="MMSVDNIKNDNQVLAEEDFLEYALSHGFGDLHFKVDPQTGMKAM
                     IAIHSTKLGPALGGCRFIEYPNTAAALKDAMRLARGMSFKAASVNLPLGGGKSVIIKP
                     KGFFNRTEYMHRFGEFVNELNGRYITALDSGTELSDMDIIAEHTDYVASLSRYNGDPS
                     PSTAKGVLRGIQAAVAFKLGKDSLKGLHIAIQGLGHVGYALAKQLHELGATLTVADIS
                     PAAVNMAVQELGAKAVGTDIIHKVPCDVFAPCALGAIINDLTIPQLQTSIIAGAANNQ
                     LAHTYHGKILHEKGILFAVDYVINAGGLIFAASKYLQTPEDKVSEQIDSIYTSLTEIF
                     TRSAKDNLPTSEIADTLAKEKLA"
     gene            33038..33694
                     /locus_tag="U6309_000322"
     CDS             33038..33694
                     /locus_tag="U6309_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5503784.1"
                     /translation="MKHLSLTPELYEYLLDISLREHPALAALRKETSTMELANMQVAP
                     EQAQFMQMLIRLTRAKRVLELGTFTGYSALAMSLALPDDGQVITCDINEGWTKHAHPY
                     WREAKQEHKIKLRLGPALDTLHSLLNESGEHQFDFIFIDADKTNYLNYYELALKLVTP
                     KGLIAIDNIFWDGKVIDPNDTSGQTREIKKLNQVIKNDSRVFVSLLAIADGMFLVQPI
                     "
     gene            33761..34807
                     /gene="hppD"
                     /locus_tag="U6309_000323"
     CDS             33761..34807
                     /gene="hppD"
                     /locus_tag="U6309_000323"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEN5503785.1"
                     /translation="MQNNNPCGLDGFAFLEFSGPDRNKLHQQFSEMGFQAVAHHKNQD
                     ITLFKQGEIQFIVNAASHCQAEAHASTHGPGACAMGFKVKDAKVAFQHAIAHGGIAFQ
                     DAPHANHGLPAIQAIGGSVIYFVDEEHQPFSHEWNITSPEPVVGNGLTAIDHLTHNVY
                     RGNMDKWASFYASIFNFQEIRFFNIKGKMTGLVSRALGSPCGKIKIPLNESKDDLSQI
                     EEFLHEYHGEGIQHIALNTNDIYKTVNGLRKQGVKFLDVPDTYYEMINDRLPWHKEPL
                     NQLHAEKILIDGEADPKDGLLLQIFTENIFGPVFFEIIQRKGNQGFGEGNFQALFEAI
                     ERDQVRRGTLKELT"
     gene            34863..35861
                     /locus_tag="U6309_000324"
     CDS             34863..35861
                     /locus_tag="U6309_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEN5503786.1"
                     /translation="MKLASLKSTRSRDGELCIVNRLLTAAVRVPHIASTLQYALDHWD
                     VVEPKLQLVYQQLNDNQIEEAFAFAPELCASPLPRAYQWADGSAYVNHVELVRKARGA
                     EMPADFWTNPLMYQGGSDAFLGPRDPILVSSEAYGIDFESEVAVITDDVPMGIDHTIA
                     AHHIKLLMLVNDVSLRNLIPEELAKGFGFFQSKPASSFSPVAITPDELGSYWDGQRVN
                     LPLISHFNGQLFGQPNAGIDMTFSFPELIQHAAKTRFLTAGTIIGSGTVSNLDRSKGS
                     SCIAEKRMLEMIEFGQAKTPFMRFGDRIRIEMLDGQGYSLFGSIDQIVTKYEKDSL"
     gene            35858..36496
                     /gene="maiA"
                     /locus_tag="U6309_000325"
     CDS             35858..36496
                     /gene="maiA"
                     /locus_tag="U6309_000325"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEN5503787.1"
                     /translation="MILYDYFRSTACYRVRITLNLKKIVYEKIEVHLVNNGGEQHSLQ
                     YHQINPQELVPSLDINGHILSQSMAIIDYLEEVYPEIPLLPKDPFMKANLKSLALIVA
                     CDLHPLNNLRVLNRLKEQFRASQEQVLEWYHHWLKTGFDAFEEKLGTMKRDKPVCLGS
                     EVSLADVCLIPQVYNAQRFHFDMASYPIINQINEYCLTLPAFHDAAPEAISS"
     gene            complement(36493..37053)
                     /locus_tag="U6309_000326"
     CDS             complement(36493..37053)
                     /locus_tag="U6309_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503788.1"
                     /translation="MLQRVLLVLLCFFSFLASASDTVLKLYRPFGDVFEQAPPVIKKI
                     MSGQCYEQSRIIIREDAWRCQAEDQLFDPCFVKAGSKKMEAVCPQSPWVGDSVQINVG
                     IPLNNEHHKTLDMSRAFPWAIELVNGEYCLAVNSNEQYDSMPVRYRCSNQNVLIGYLQ
                     RCKTAWSMLEKTPKGVITVELRRAWF"
     gene            37201..38604
                     /gene="asnS"
                     /locus_tag="U6309_000327"
     CDS             37201..38604
                     /gene="asnS"
                     /locus_tag="U6309_000327"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEN5503789.1"
                     /translation="MTEVFTIKQCLDGEISIDETVTVRGWVKTRRDSKAGLSFISLHD
                     GSCFSPIQIVATDQLSNYHKEVTKLTAGCSMIATGKLVASQGKGQFFEIQAESIEVVG
                     WVENPDTYPIQAKRHTLEFLREVAHLRPRTNTISAVTRVRHSLAQAIHRFYHEQGFFW
                     VHTPIITASDCEGAGEMFRVSTLDLLNIPKNDKGQIDFSKDFFGRETFLTVSGQLNVE
                     AYCMAMSKVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFANLEDICKLSQNMLRYLCK
                     TVLEERADDMDFFNQFVAPGCIERMEHIADSEFEIMTYTDAIKALEASDQKFEFPVSW
                     GLDLQSEHERYLAEVLCKKPVIVTNYPQEIKGFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEILDRRMDECNLNKEHYQWYRDLRRYGTVPHAGFGLGFERLISYVTGVS
                     NVRDVIPFPRTPGHADY"
     gene            complement(38601..38999)
                     /locus_tag="U6309_000328"
     CDS             complement(38601..38999)
                     /locus_tag="U6309_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503790.1"
                     /translation="MDEKKDKTIQINEITSTVEDEKKAQPLRKSERSLPVKSHRRSFS
                     IFYPHSTENSKIPSSAQTSNKGFLPGPDFNREHYDTVDAGGPLASNADDEILEKIFSP
                     RNRRRLLPESHQPQQQTVEVDDDSFRPLPI"
     gene            complement(39023..39706)
                     /gene="lolD"
                     /locus_tag="U6309_000329"
     CDS             complement(39023..39706)
                     /gene="lolD"
                     /locus_tag="U6309_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEN5503791.1"
                     /translation="MNDIILTSQKLYKSYHDGTSTVEVLKGVDLAITKGDRIAIIGPS
                     GSGKSTLLHLLGGLDKPTSGLITLGNVNWQKINEKQRCQLRNQQLGFVYQFHHLLPEF
                     TALENVMMPLLLAGMAVKGAEEKAINMLEQVGLKPRLAHKPAQLSGGERQRVAIARAL
                     VHQPHCVLADEPTGNLDEATASKVFDLMLELNKKMNTALVIVTHDQRIAERMDRVLVL
                     HEGSLCARE"
     gene            complement(39699..40946)
                     /locus_tag="U6309_000330"
     CDS             complement(39699..40946)
                     /locus_tag="U6309_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEN5503792.1"
                     /translation="MYKPLALFIGLRYTRSRKKNHFVSFISLSSMLGIGMGVMVLITV
                     LSVMNGFDQEIHRRFFGMAPEITITGPDERLSDWPEVVKKVETIPGIKAIAPYVGSQG
                     LLTHEGQVLPIVLTGILPEKEQSVTHLNKKLLAGNMDNLKHFGIILGKGLADSLGVMI
                     GDKVTIMIPQATVTPAGMIPRFKRFTVVGVFSAGTGFNFDTKLAFINIEDAQKLMQLD
                     KNDVSGIKMKINNVYKAPELSYELSDLLGEGYQVGNWTQQFGAFFEAVKMEKTMMFMI
                     LLLIIAVAAFNLVSSLVMVVNDKQAEIAILRTIGATPSTILWVFIVQGMMVGLVGTIL
                     GLLGGLVLANNATEIVNALQSFFQVKVLSSSIYFVDYLPSKIMFRDLWQVCAMALLMS
                     FAATIYPAWRASKTVIAEALHYE"
     gene            complement(41386..41472)
                     /locus_tag="U6309_000331"
     tRNA            complement(41386..41472)
                     /locus_tag="U6309_000331"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41436..41438),aa:Leu,seq:taa)
     gene            complement(41489..41562)
                     /locus_tag="U6309_000332"
     tRNA            complement(41489..41562)
                     /locus_tag="U6309_000332"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41528..41530),aa:Cys,seq:gca)
     gene            complement(41623..41698)
                     /locus_tag="U6309_000333"
     tRNA            complement(41623..41698)
                     /locus_tag="U6309_000333"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41663..41665),aa:Gly,seq:gcc)
     gene            complement(41751..42314)
                     /gene="pgsA"
                     /locus_tag="U6309_000334"
     CDS             complement(41751..42314)
                     /gene="pgsA"
                     /locus_tag="U6309_000334"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEN5503793.1"
                     /translation="MSTLTSLPNILTLMRISIIPVFIMVFYFPFKWAHPTAAVLFAIA
                     SITDWLDGYLARKLEMMSHFGAFLDPVADKLLVSTSLLLLVGAKDIDYITIPAIVIVG
                     REIVISALREWMAEVGSRASVAVGYIGKVKTSLQMIALVLLLAFNPLVSWWGSLGFVL
                     LYVSAILTIWSMVIYLAIAWPELMKKS"
     gene            complement(42334..42993)
                     /locus_tag="U6309_000335"
     CDS             complement(42334..42993)
                     /locus_tag="U6309_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEN5503794.1"
                     /translation="MSSAYQLVIFDWEGTIADTLGVILHTVATEAKMLGFGDIDPYQA
                     RKYVDLGLVQAVKKTLPNLTAGQQEDLLQAVQSAMISRPTEVRLIPGVLEFIQQLHQA
                     KVDLAIATNKGHNSLIRALQATELDQLFKVTRSAGQVPAKPCPQMLEEIMEEFGRDPS
                     STLMIGDSTTDMEMAKRINVNAIGMDFYHQQEDALKAAGALAVFDDYKLLSAYLMLPE
                     A"
     gene            complement(42986..43933)
                     /locus_tag="U6309_000336"
     CDS             complement(42986..43933)
                     /locus_tag="U6309_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN5503795.1"
                     /translation="MSEVSYKEIGSEENGQRLDNYLIRILKGVPKSHIYRIIRGGEVR
                     INKKRAQANSRLQIGDSIRIPPIRMTDSKEIFVSDNLAKRLKDSIIFEDSSLLVINKP
                     AGIAVHGGSGLSLGVIEALRKTRHDLSYLELVHRLDRETSGCLLLAKKRSTLRAIQAL
                     LEAREVQKIYWALLTHRWDGKKKITITAPLEKNTLKSGERVVSVQKDGKASETDFKLL
                     ENYHQACWVEASPKTGRTHQIRVHSAYFGHVIVGDGKYGSKVGEVEGIDYQQNRLYLH
                     ARSIQFNLNGVMHVFQADVDDGFGNTLKQLRARSLLSYE"
BASE COUNT        13555 a         9348 c         8088 g        13013 t
ORIGIN      
        1 atatgagcaa tcattaagaa gttagctaaa tgaaaagagt aaactttata cctccagagc
       61 ttaaggacta ttgtataaaa acaatccatt ttcatcattg ccattactga tatcacaaag
      121 ccaatcgata attaacacac ttgtgcctaa agccggatta tagaaattca cacgcaaggt
      181 ggcagtatta tcttcaaaga cattcaaacg acttgaaaca gataaaaata cacccggcga
      241 atattcaaat atcttgcttg agacagtagt taccgtatct cgtaattgat cattaattct
      301 ggttcgaaaa tgcaaatact ctgtaaaatt gaatcttgtt gaagcgccat ccaaattctg
      361 tactaattga gtttgcagtg cgggatctgt aatttggcat cgatccaatc gtataatggc
      421 cttgacatca tcaccttgtt caactgcttt aactaattgc gcaaaattag tcaacaagga
      481 agtgttagtt aacaaagaag taattgcatg ggcatggcct ataaataaag acaaataaat
      541 taacaaaaga aagctattcc atttcttcat aacgtcccct cttattgatt cattatttgc
      601 ttaatctata agaacaaata ttacataaag tgtataatca acaatcgata tttgccagaa
      661 aatttcattt tcttttaaga aaattgggga ttttatgtaa gtcgtgtaga atttagtacg
      721 cttttttaaa aggtaataaa aacaataata ttttgggctg atgagaaagt aagcattcac
      781 gggtataaat ttcgaggtca agttaaaaag atcaaaattt tttataaact ctatattttc
      841 aaaatgggta tccttttgtt tctttagagt aacgtgagtg atgaataaag attatataag
      901 aaccagtcga tacccaggga gccgttcagg ctgaggagat gcataagcat cgtcttgaat
      961 tggtatgaaa cattaaatct cgtacaaggc ttcgagacgg catataaata ccctctcagc
     1021 ccgagcggtt tgagataaca actgatttta tccataactc aagttagtat agatacatgg
     1081 aattcaagtc atacatttaa tccaaagaga cactatgcaa aacatacagc acctgattga
     1141 ctgcattctt catatcgatg tttatttaaa tgcctttgta tcaacatatg ggttttggac
     1201 ttatcttgcg ttatttgctg tcatcttttg tgagacaggc cttatcgtca ctcctttctt
     1261 accaggagac tccctgttat ttgctgcagg aagtatagca gctcaaccag gaaaccctat
     1321 gaatatcatg ctgttgtttg ctttgctttt catggcttcg atattaggga atcaagtgaa
     1381 tttccttgtt ggacgtgtaa taggccctcg tgtctttagc gccaagcgct cctggttact
     1441 gaatcccaag catctgcatg atacccatgc tttttatgag aagcatggcg gcaaaacaat
     1501 aatactggcg cgttttattc ccataatacg cacctttgct ccatttattg caggtattgg
     1561 caggatgcga tcattgcatt tcacgctttt caatctgatc agcgccatac tttggattgg
     1621 aagtctcctt agcctgggat attatttagg ctcaatccca ttaataaaag agaatttctc
     1681 attggttatc tatgggatca tagcgatttc aattttaccg cccatcatcg ctttaattag
     1741 taaaaaatca acgtcctcaa ttaaaaaata atatactcta gggacttatg cgggtgcaaa
     1801 gggctctaag gatgcctttt gcaccacgct cttactaggg aacatatctg ttggcaaaca
     1861 attgcacttc taaaacagat gctagtttat ttaaagaaga agtaaaatgt ctggacaaat
     1921 attgcaaatc ttttttattg tcatgattcc cttgtaattc atgcagtaac attgttctgt
     1981 aggaatgtgg atgtgtattt ccattcctgc tgtcatttaa ataatgaacc aaagcttctt
     2041 tggcttggcc ataatctgtt attttagaaa atgttttgca gaagttatta gcccgatctc
     2101 ggcctgtctg accatgataa tgaaagaagc aaaaccagat gctttcactg taggtggtgt
     2161 aagcgagcgt agcattggca acaattcgtt ttaattgctc caattttctc tgctcttcac
     2221 taactaccgg tctagcatgg aaaaaccctt tactgattgg tttattggtc tctacctctt
     2281 tcttatgatg ggggttagat atttcatcaa aaggaggtat cataaaatcg ttggctaatt
     2341 cttttccagg aaactcctcg ttaactcgct gttgctcccc ggttctttta gctccactca
     2401 ggtgagggtt tcgccccaaa agcattcctg tagtggctct cgccgttgta atttgggtag
     2461 tccaatgcgt attattacta ttagttaaag taagagtgtg tcgctcggga atatcgacaa
     2521 actcttgaat tggactatta ttccttagca catgaagatt cacctcaaac gcttcggcta
     2581 aataatttaa atccaaatgg gtgccccaga aaaaatcctg agtgatgtta ttcatcgcag
     2641 taatgatagg tgaatcactc tttggttctg tttccagggt gatatcagct ggattcgtag
     2701 cgtcaccata taataaattt aaaaagagag gaaccaatac atcgttttca tgtcctggat
     2761 gaacactgtc tcgatcaatg tttgccattt taatcagtat aggaacagaa tcggcaaaag
     2821 gattgaaccg agaatctgta tccagaggat cgccataata taaggctgca aaattaacaa
     2881 aattgctgtt tccagttaat cgattataat ttccatttct gaatacacag acatttctta
     2941 attctcgtat ttgatattga taggtcacta ttcttaagga gctttgcaac ctgtccagca
     3001 attctttatc gggatcttca agattaagtt tcaaaatctc atcgtattga cctgaaattg
     3061 acctatcgag acctacccat cgatcaaaca tggttcttct attatacttt gcctcttcct
     3121 gaattatatt caccaaacct atcgcaaagg cataaaaagc gcaattgcca aggccagggt
     3181 tatcaatggt tctttctctt aagaaaacgc caggctcttc gatcaaatcc aattgctcag
     3241 atgttggtgc actaacttgt ttatcacctc gataagcctc cagggaagtc attttcaaac
     3301 gtgagggtgt cgaatccaga aaacgctcgc tatcttcttc attacacaat acattccaat
     3361 gctcttcctt gttctctaat ctgattttga ttcggttgtc cgtaatgtcc tcttctctat
     3421 tctcaaccag actgatcgct aaggcatctg tcataccctt taactcatca cagctataac
     3481 ccctttgaaa ggcatgagga tctgaactga tctttttctt taaagcggat aataattctt
     3541 tagccttttc ggtcagaaat tgttttacat tttcacgtgt cagttcaagt ttgtagtctt
     3601 taagaatttc tcgtaattta tctaataaag gaaccctgtc tttgttcaaa tccagatttt
     3661 ccaatttggg aaaaagagcc tcgcccaaat cccctgtgtt ttgcaattga aaccaggctt
     3721 ggccattttc aatattggcc ttatttttcc atacaagatt ggttaattca tttagaattt
     3781 cttcatccag atgatcttga tatttctgat tcaaattaaa taatacgggt gccaaaatca
     3841 gctcaatgtc tctggtatcg ttgtaatagg ctagccactc ttttaaagtt ttccagcttt
     3901 tggggttaaa ttgcgggtga tgtttggcaa attcatccag caagacctga taaccgggtg
     3961 tatccagctg cgaatccagt cttccgctta agatctcgac actcagtccg attgacactg
     4021 cattaaatag acaagcgcca tctccagtag ccttaattgt cttcgccata catactcccg
     4081 atagttctgg tcagtgaata aaattaatgt tcacatattg tactctgagg gggtataata
     4141 atcaattaaa aacagctcat caggctttta aaaaattaac tatccactgg gcaataattg
     4201 tgtcatcctc atgtaactca agagatttca tcccttttgc aaattcaatt tcattgaccg
     4261 aatcctcacg agacaccatc aaatcacgag aataagattt agtaaactca gggtgcccct
     4321 gcacactaaa gaaagaacca atttgcatca tataatttgg acaaaaatca ctccctgcta
     4381 atatctccgc accggttggt aactcaatca cctgatcttg atggctaact agcaagttga
     4441 agcaatttag agatggtctc atccattctt tgaattggta aatttgattt tgtgacatac
     4501 ctacccccca acccttggga gatttaatca cttttccacc taaggctttc gctattaatt
     4561 gatgcccaaa gcaaatacca attaattttt tttgggaagc atgcaaagta cgaacaaact
     4621 cttcaagctt tcttatccag ggataatcat cattcacccc atgtcgactt cctgatatca
     4681 gataagcgtc tacggcatgg atatctgttg gcagctcccc atgctctgca tcaaatacag
     4741 taaattcaag agtagaatcc gctgggcgca atagattggc aaacatctca gggtattgac
     4801 catgatccgc aacaaatact tcacttacct tatcacaaag caaaatacca agattcattt
     4861 aattcaccta attaattatc gcaaaattta tattgtctgg attagactta atttcaatat
     4921 acaagtcctt ttcatctgat ttatacctgg caaacaaccc gtttctgcac cttttccttt
     4981 acaacgatta ctcaccaatg gctttgctca atagttctct gtattgctcg aatgtaaaaa
     5041 gaacaggatt ggaagctgct gccgcatcct cagtggccat ttttgccagt ctgtctatct
     5101 gcgaatgatc aatacctaac tgacttaatg tattttcgat tcctaactct aatctcatct
     5161 gctctatcca atccaaaaaa ccatcaaatc catttgctat tgataaataa tttgccagac
     5221 gctctatctt gttttcaatt tcactacgat tagccaataa aacatagggc atcaaaactg
     5281 catttagccg cccgtgatgg acatcataaa ttgcacctaa tggatgagcc aaggcatgca
     5341 tcgctccaag accacgctga aaagcggtag ctcccatagc tgaagcaact agcatatggg
     5401 ttcttgcctc caggtcacta ccatccttgt atgcttgaat taaattctcc ttgattaatc
     5461 ttattccttc caacgcaata ctttctgcca taggatgata ataattggaa caataagctt
     5521 caagacaatg ggaaagagca tccatacctg tggctgcagt gagtaatttg ggaagcccga
     5581 ctgttaactc tggatccaga ataaccatgg caggcaacat tttaggatga aaaataattt
     5641 ttttaacctg cttttcagca tcggtaatta cagaagctcg cccgacctct gaaccagttc
     5701 ctgctgttgt tggaatggca agaactgggg ccatggctga aacattcacc cgagtccagt
     5761 tatcacccac gtcctcgaaa tcccataaag gtcgatcctg gccaaccatt aaagcgatgg
     5821 ctttgccggc atccaaagcc gagccacctc cgaaagcaat cacgccatca tgctggccag
     5881 ctttaaaagc acagactcca ttcatcacat tgtcaccggt aggatttgtt ttaatttggc
     5941 aaaataaccc cgttcgtaaa ccggatgcct gagcttgttg tatgacttta ctcaccatta
     6001 acgacgaagc caatcccggg tctgtgacta acagaggagc attcatgcct aagtcacgac
     6061 atgctttcgc tagttcattg attcgcccag ccccaaccag gattcgcgtc gggtaattcc
     6121 aattgacaat taatgattgg tttttcatgt atgatcttcc tttgattcga aaaagaccgt
     6181 tgtaactgat agcaagtacc tttccgcagg aaagattagg tcttttttat aattttattt
     6241 tgagttttaa gacgtaagta atcgtaggta tcaggcttta agtctcaatt ttagttctga
     6301 tatggaatga tttgggccgc gttaaatact catagcctat ggcggataaa gtgcaacccc
     6361 tgcctgattt ttttacacct gtccaagcca aaccgggatc aaggtagtcg caacgattga
     6421 taaaaaaagt ccctgtttgt aatcgttcac caatcgctat tcctcgatga atatcctgag
     6481 taaatacagc agcggtcaaa ccatagtcac tatcattcat cctttcgatt gcctcttcat
     6541 cactatcaac cgacatgatc cctacgactg ggccaaaagt ttcttctgtc ataattcgca
     6601 tgcgatgatt aacatcggtt aaaatttgcg gtgccagata gggtgtgcca ggtctatcca
     6661 ttacaaaatc ctgggtattt atatgtgcta ttgccccttg ggctatcgct tctttgattt
     6721 gctctctgac aaagtcggct gaggaagcac gtaccagtgg acctagagtg gtttctggat
     6781 catcgggccg tcccaattta tactgtttaa ccaaattaac cgctttatta acaaattgtt
     6841 catatatctc tttatgaaca taaatccttt ctataccaca acaggattga cccgaattaa
     6901 aaaacgcacc atctatgact gtttctactg catgatccaa atcagcgtct gctctgatat
     6961 aagccggatc ttttccacct agctccaaat tcacaggaat gaaacgtcct gccaatgtct
     7021 gttcaactct ttcaccacca gcaactgaac ctgtgaaagc gacagaatta actgctggcg
     7081 atctcatgag ttgttctgta tcttcatgtg tcaaatgcaa atattgaaaa acacccatgg
     7141 gtaagcctgc ttttttgaat gcctgatcga aacgctcagc cactaacgga gtttgcgcag
     7201 aatgcttcaa aatcacaaca ttacccgcca taagtgcagg aataatcgca ttgaccgctg
     7261 tgagataggg gtaattccat ggcgctataa ccaacacaag ccctaacggc tctcgtttga
     7321 tatatcttgt aaatccttct ttttcaggta attccacaga agccagcgat gattcggcct
     7381 gtgcaatcat atatcttgct ctctcttcaa acccattgat ttcaccagct gcgtatcgaa
     7441 ttggtctacc catttgccaa caaatttctt ctgcaatgtc tactttgcaa gcaactattt
     7501 cattgacagc agccaaacaa tattcctggc gttcggccag agaagacaaa gaccacagtt
     7561 tttttgctac ttgtgctttg cttaaaacag tttttatctc ttcttgattc gcatactctc
     7621 tctcaacata aagagagtta tcgacaggag aataggtttt taaggtagca atcattttta
     7681 atccataaat tttataaaat ctgtcgtttc aattaaataa tttcaaaata cctgtcgagt
     7741 tcccaatcag taatatgttt tctaaactct ctctcttccc attctcttga agcagcaaaa
     7801 tgctcaacaa attcttgacc aaataattca taagcagcgg aagagttttt taaattttgt
     7861 gccgattccc ataatgtgcg aggcaacgcc aatgccgaac tatgattttg ctcgtaggaa
     7921 ttccctttta cctcagcctc aggctccaac tcattctcaa tgccatataa accggaccct
     7981 atggcagcag ccaacgccag atatgggttt gcatctgcag aacctagccg atattcaata
     8041 cgctgtgact tatcactgcc gggaatcact cgtaaagcag tggtgcggtt ctcgacaccc
     8101 catgttgcct ctgttggagc ccaatagccg ggaattagtc tggaatagct attcacagtc
     8161 ggggataaca tggctaaaaa ttcaggcatc aatttttgct gccccgctaa aaaatggcgt
     8221 tgaattttgc tcatattata ttctctgcct gattcataaa aaacagactt gccgccatct
     8281 ctatctcgca aagacagatg tatatgccca ctttgcccag ggtaatcacc tgaccattta
     8341 gccataaaag ttgccatttt attattgcgt tgcgcgagaa ttttcataaa tgttttaaac
     8401 aaagccgctt tatctcctgc tgcctcagca tcatcaacag caatcgctgc ttcaagaact
     8461 cccgggcctg tttcagtatg taaaccttca atggggaaat ccattttttc cgccatagtg
     8521 agaatttgat gataaaactc agcatgtacc gtattacgaa taattgagta gccaaaattg
     8581 tcaggagtaa caggctttaa attacgaaat ccttttgcac gcacgctgtc aggtgtttca
     8641 tcaaatacaa aaaattcata ttccaatgcg gcataaacat cataccccat acccgcagct
     8701 ttattgatta cacgacgcaa gatcccgcga gggcagatgg cttctgctgc ttgtgaaaat
     8761 tcagccataa acaacaattg attttcttca aatattatgt cacgacatgt tgaaggaaca
     8821 attcgcactg aggcgtcagg ataacccgta tgccagccag tatatttcac attatcataa
     8881 agcttatctt tagaatccca tcctaaaatg acatcacaaa aagcaaaacc atgctccaaa
     8941 gccgaaaaaa acttgttacg gctcatgtac ttgcccagca tcacaccatc gatatcaaaa
     9001 agaccaacct taacatgagt taattgtctt tcttccacga tgcgttttgc atcttctatg
     9061 gttcggatct ctcttgggtt tagcatggca tatccttatg tcgttctgtc aatgtaagct
     9121 tctttacctc ccaatgtcta attactacaa atacgacact gagacaataa cccattttga
     9181 atccagtttt ttaaataaga cgctgctttc attccgacct cttcagcagg catccactgg
     9241 cataatccct cacaaatcac cccaaaagag gagccctgaa tcagagcgtc taaagcccac
     9301 gcttcttctt ccgacaagct gtaatattga atgatataat tttgaccccg ccataacacc
     9361 cagccagtag tagcaggact atctttcaac tctggaattt caagattatt gaccaaagct
     9421 tgccacaatg aaaaaacatt ccaacaaaaa tcaagcctat ggactgatga atgaagtaca
     9481 aaccccatct cagcccagga ttcgggcggc actgctgcca tctcttctac attaagcact
     9541 gtatcatctg ctgcatcgaa ggctaaggtc attttccatt ccagctcagc cagttcatac
     9601 aaataatagc aagacttcgc atagtacttt tttatcaatt caggcaatct atcaccatac
     9661 catcggattg aacgataagc agatgggtaa gaatcaatat aacgagcact taacttttca
     9721 aattcctcag tacctaaata cgcatataaa gcggggtaat ttgctgccaa acattcaatc
     9781 aatctcaatc gataaccgtc tttataaata ttcagacgcg tttctgttga aactaacttg
     9841 gttggcacta tggattcttt aattgagccc tgcccggaat aaatgaattc ttgaaattgg
     9901 gcttgtaaag tcaatagcga tttcatacag ctaccctttc ttttgcagct tgtatggcaa
     9961 ggcgttttgc atgattaatt tcacttaaaa gctcgctaaa atcaggcatg ttatcatcac
    10021 gttcaatcat agtagataca ggcccaagtc tctgtatggt tgcctcataa agatcccaaa
    10081 ctggctcaat cactggagca ttgtgagtgt ctataatgta atctccgtga ttagtgtgac
    10141 ccgccaaatg gatttgagct actcttccag gaggcatgga attaatataa gtcattggat
    10201 cgaaatgatg attgaatgaa ctgacataaa cattgttgac atccaacaat aaataacaac
    10261 cggattgttt tactatttcc agaataaaat cccattctga catttctgac tgtttgtatg
    10321 taaggtagct tgatacattt tcaattaaaa aaggacgttt taagaaatct tgtatttctt
    10381 gaatacggga tacgatgtgt tgaatagctt cggttgtata gggaacaggt aataaatcat
    10441 gtgtatttaa tccatgaacg ccagtccagc ataaatgatc tgaaatccag acaggctcaa
    10501 tgcgagaagc taattcttgt acctgcttca aataatccca attcaaagga tcagtacttc
    10561 ccagggaaag agagacgccg tgcatgacaa tagaatagtg ttcgcgaatt ttgtccaggt
    10621 aatagagggg cttacctccg gggattaaat aattttcagt taatatttca aaccaatcca
    10681 gatcaggttt gctcgtcagg atttcttcat aatagtttgg tcgcaatcct aaaccaaaac
    10741 ctaaatacgg taatttttga tctgacttat ttggggatac ttccatatta aacctaaaaa
    10801 agaacgggga ccaggcatgt ttttctacat gcctggcaag atgcgactga attaattgct
    10861 attattcagc aacagtacca ccttgttttt cgcaatcttc tttagacatc tcaagaatac
    10921 ctttgccttt gcaagaattt tgacctttgc aagcattatc agcagtttta cagctgcctt
    10981 gccctttaca agcattaaca cctttacagt gaattgttgt ggttgtagca tcaacagcaa
    11041 atgcaggagc caaagcaaac atagaagccg cgccaacagc taaagcagcg cctgttaatt
    11101 gtaatttatt catagcaatt cccttttgat taaatgaaaa tgattgtaaa cccggcaaat
    11161 ctgaattgtt ttgccaatag cattttaaga atcaatagtc aaaaaagtca agattatcca
    11221 aactgataaa taattatttt ttcaaatagt taagtaaatt atttaattac actacaaatt
    11281 ttgacagtac tttatatcac caactcccgc cgtttaatcg aacaggttcg ggagacaatt
    11341 gaatcaattt gcgattttct aacttccgga tcattaagcg gaattgacct ctaatccttt
    11401 caataaaagc cattatttct gcaccggaaa aaatcacacg ggaatattgc gaaaaaactg
    11461 gaatatgaat gattgttccc tgtttttcga gtctctgtag ctcttgctca gaatattaaa
    11521 tcgaatgtta ttttggggat tcgttaatcc aaatttactt tttcaagata gttaggtatc
    11581 ttgcaattcc atcctagctc cgcttcaata tgtcccttta accctaatgc cgcttgaggc
    11641 tcgccatggg taataaaaac ttcctttggg gctttctcaa aatgctttag ccaaactagt
    11701 atttcctcat aatctgcgtg agcagaggca ttggttaaca tagctacctg agccctgact
    11761 ggataatcat cgccatggat tttaacggac ggctcatgat ttaacatttt ttcacctctc
    11821 gttccagcag cctggaatcc ggtcaaaagg atagtacttg caggatcagg agcatatacc
    11881 ttcaaatgat gcaaaaccct gccccctgtc atcatgccac ttgcagaaat aataatttta
    11941 gattcgcgcc cccaatccac tgccatggat tcatttgggg tatggacata agtcgctatt
    12001 tggcagacgg ctcgacactc ctctgcactt aagcggtgtt cgttatgatg ttttaataat
    12061 aattcagtgg catcaatagc catagggcta tccagataga cagggacatc aggaattaat
    12121 tttttttgtt ttaaaatata caaataatac aacatagttt gtgctctacc gactgcaaaa
    12181 gcaggaatta gcactgtacc acctctttta caagtcgtat tgactatgtt ggctataaac
    12241 gctaaaggat cggttttatc atgcaagcga ttcccataag ttgattcaat caccaaataa
    12301 tcggtagatt ttattaactc ggaaggcttc ataacaggat cattgagtct tccaatatca
    12361 ccactaaata ataacttgat ttgctttgtt tgaaattgaa taaacgcaga ccctaatata
    12421 tgccctgccc tatgccaact gatttcacaa tcctccatta aaggataagg ggtgtcatat
    12481 tctactgatt caaaatactt taaggtatta tttgcatctt cctgagtata caaaggtaaa
    12541 gccggatggt gcttgctgta cccatattta tttgcccttc tggcatcttc ctcgtgcaaa
    12601 aaaccgctat caggcagcaa gatagaacat aaatcggttg ttgcagctgt tgcatagatg
    12661 cgccccttaa acccctgttt caccaacaat ggcaaataac cactatgatc aatatgtgca
    12721 tgggtcaaaa caacggcatc tatcgattct ggagacacag gcaggggtga ccagtttctt
    12781 agtctcaatt cttttaaccc ttggaaaagt ccacaatcta ccaaaatctt tttattattt
    12841 gattctacca ggtacttaga accggtaacc gtaccggtag cacctaaaaa agacaatttc
    12901 ataataatac cttgttgtat gtcattctca gtactgtaat gaataacacg gcctattgcc
    12961 aatttatttt aaagtcattt gaagtttcaa attgtaaatc tttatcccac ccattcactt
    13021 gcacttatgg tgtggaggct tttataaaac tgctttttaa gttttttacc cacttgtctc
    13081 tattctcttt agtcatatcc gattttttaa ttgcataatg aagcacataa attccatcct
    13141 tgcccttggt aattttcaga atttcgtctt gttgcagatt ggcaggctct tgaattttaa
    13201 actcaaaaat gagtagattg ggttgctcct caatgacatg gacaatatat tttgccactg
    13261 atttattcat attgtgcaaa aattgattgc caaactgagc tggagtcatg ttgattaaac
    13321 ctggcataaa ttgtgtagta atcaactctt tccagttatt aatgtcatct ccaacaggaa
    13381 tatactcaat ggtaaccacc ttatctgttt tctttcccca agcgatcttc catggtctgt
    13441 catcaaaacg cactatttgc ttctcataat ggataacttg tgaatcacct gaggaatcag
    13501 ccaaaggaac aacactgcaa taaagttctc ctttataggt tacaaactcc acacatttag
    13561 gattattttc tttattgact ttgattaatt cttttggaag agaagagcca ttaggctctg
    13621 cgtagcaaat aacggcatta agtaatccaa aacataaccc aatgaaataa ttcatgctct
    13681 taatccaaaa agtggatact atttaattgt agacgttgaa acccataacc cacaattttc
    13741 attaggcaaa ctggtatata attatattag agagctaata aggagattgc catgaatggt
    13801 tgcaaaatca gtcctgttgg actgggtctt tctttaggaa ttctatgggg attatcattg
    13861 ttagtcatgg gattaatcgc ctatttttat gcctatggca ggccctttgt tgaagcagtt
    13921 gccaccttat accttggcta tgaaccttct atcctgggaa gcttcattgg tggcgtcatt
    13981 ggctttattg atgggttcgt cacggggttc cttatcgctt ggctttacaa ttgttttagt
    14041 tgttgttctt gttgccgagg caaagaaaat aaaaaagaat aagctttcta ataatgagaa
    14101 atcatgacgt caatgacgtt aaaaccaacg tcattgacaa gggtaattaa tttattcaat
    14161 agttaaaatc atttatggac gaacaataat acggttctct actgactgaa ccccttgaac
    14221 ttgtctggca atttgttctg caatatcgct ctggcgaatt tttttcacat aaccacttaa
    14281 tatcaccaca ttttgattgg attccacatg aatttgattg atgacaggat cattactttc
    14341 cattagtgaa tcctttaccg actgagccaa agtcatacct gatggattag acggaataaa
    14401 catgttactc acagtattgg tctgacaacc tgctgtaaac aaaatcaaaa acacaatgat
    14461 tacactggat aaacatttta gcatcacaac ctccaagata gagtggattt atttacactg
    14521 cattaaaaga accaaataat agcacaactc cccgtatttc tatagtcaag ccactgccta
    14581 taaatcatcg tttgccaaga gaaacttaag tcttttcaaa cgataactca gattaaattg
    14641 tcatttatgg aatgctgcta tactattttt tataaatcaa acacctacag gacgcaaggt
    14701 atgaaaaaaa caaccgaaaa acctatcaat aaaacaaaag aaatgcctgc atcctcaaaa
    14761 aaagaaaccc taaatccact ggaacaaaca ccttctcccg aacaatccga tcccattttc
    14821 agatttattg ataagttata tcaagctaac ctgggaaagt taactgctgg tataagtcct
    14881 gctgcgctag gtacagccta ttactcctgg tttgctcaat tgctacagtc acctggttca
    14941 atgctacgat tggcttatta tccgctgttg catgccaacg attatctttc caaccttttc
    15001 aagtatgaca agcccagaga tggcaaggac gtacgttttc atacagaaaa ctggagttat
    15061 tatccctggc ggctttgggc tgagcaattt ctccaatttg aagattggtg tttacaagcc
    15121 tcaagcaaag ttccaggtat accacttcat gtcaaaagaa cagtaacctt ttccaccaga
    15181 cagattcttg acgccttgtc accttccaat tttgttttga ctaatcctga tttgcttcag
    15241 gaaacaattc gctcgaacgg gcaaaattta attcgcggaa cagagcttgc ctttcaggat
    15301 tttgtagaaa agataacagg ctcacctcct gcgggagtcg agaattttat cccggggaaa
    15361 caagttgctg taacaaaggg gaaagtcgtt tatagcaatc atttaattga attaatccag
    15421 tacacacccc aaacagaaaa ggtatacaaa gaaccaatct tgattttacc tgcctggatc
    15481 atgaaatatt atatcctgga tttgctgccg gaaaactctt tagtaaactg gctggttaga
    15541 cagggacata ctgtatttat tgtatcttgg cgcaatccaa cgaaagaaga tagaaatctt
    15601 ggactggatg attattataa gttgggcgct atggatgcca taaatgcagt gagcaatgcc
    15661 attccacata ccaaaatcca tttaatgggt tattgtcttg ggggcaccct ggctctgtta
    15721 actgcagcag cgatggccca tgatcacgat aaccgtctta aaacattatc cctgctcgct
    15781 gctcaaggcg attttattga tgccggagag ctactgctct ttataaccaa aagtgaagta
    15841 tcctttttaa aaagtatgat gtgggagcaa ggatatctgg acacaaaaca aatgtctgga
    15901 acttttcaaa tgctccgttc gtacgatttg atttggtcta aaatggttca ggattacatg
    15961 cacgggactc aaagagggat gatcccttta ctggcatgga acgccgacgc aactcgcatg
    16021 ccatacaaaa tgcatagtga atacctggaa aaactgttcc ttaataatga ttttgccgaa
    16081 ggccgattta ttctggacgg aaaacctgtc gtaggagaaa atattaggat acctgctttt
    16141 gttgtaagta cagaaaaaga tcacgtggcc ccctggaaat cagtgtataa aacccatttg
    16201 ttaatcaatt ccgatattac ctttgtcttg actaatgggg gtcataacgc tggaattgtc
    16261 agcgaaccgg ggcatgaggg acgttattat cgtattcggg aaagaaaaat ggattcaacc
    16321 tatcttgacc caacaaactg gctaaaaaag gctgaattaa gagaaggatc ctggtggatt
    16381 gcctggcacg attggttagt taatcactca tcccaaaaac aggtgtctgc gcccaaactg
    16441 gacaaaaact tgcccaatgc tcctggaaaa tatgtattac aaaaataaag gaattagtaa
    16501 tgaataatga atttcttgaa aatgtcacct atgaagaact gactataggg cgcaaggcca
    16561 gcttaaaccg tacattaact caggatgata ttaatttatt tgctgcaatg tctggagaca
    16621 tcaatccatc tcatgtcgat cctcaatttg ctaaaagcga tgtattccat ggcgtagtcg
    16681 gacatggcat gtggtctgga gctctaattt ctaccttatt gggcactgcc cttcccgggc
    16741 caggaactat ctatcttgaa caagatattc aatttataac tcctgtccgt attggggata
    16801 atcttacaat taccatcgtt gttcgtgaca aacatccagg caaatcgatt gttcatttcg
    16861 actgtatggg ggtgaatcaa aaaggagaaa cagtaattaa aggccttgcc aaagtcattg
    16921 ccccgactaa aaaaatgaag gtaccccgaa ctgttttgcc tctggtgcaa atccatcaca
    16981 atgatcactt taataaaatc attgaaacct gcagtaactt gccctcaatc aaaacagcag
    17041 ttgttcatcc cgtcactgcc aatgtcctgg aagcagttta tgactcagtc aaggccggtt
    17101 taattactcc tgtattaata ggtcctatgg caaaaatcaa atgtgccgca agtgaagcta
    17161 aaatcgattt atccaattgg gaaatgattg atacagaaca cagtgatgct gctgctttta
    17221 aagcagttga attagcggca tccgggaaag tggattctat catgaaaggc gccctgcata
    17281 ctgatgagtt aatgtctgca gtggtacctg ccacttctgg actaagaacc aaataccgca
    17341 tcagtcacgc ctatgttatg gatgttccaa cttaccataa acctttaatc attaccgacg
    17401 cagccattaa tatagcccca aacgcagcgg ataaggcaga tatatgtcaa aatgcaatca
    17461 atctctggcg tattttatat ggagaagaaa caaaacccaa ggtagctatc cttgcagccg
    17521 ttgagatggt aaatccaaag atgcaggcaa cagttgatgc cgctgtcctg tgtaaaatgg
    17581 ctgaccgagc tcagataatc aatggcatac tggatggccc cctcgccttt gataatgcca
    17641 tcaataaaca agccagtaag gaaaaaaaca ttatctctcc tgtagccggt gatgccgaca
    17701 ttttgctggt accggacatt gaatcaggaa atatgctagc caaacaactc acttttctcg
    17761 gacatgctga cgcagcagga atagttctcg gagcaagagt ccctatcatt ctggccagca
    17821 gagctgattc gttaagaaca cgtttgttat cgtgtgcagt agcaaccaag attgcagcgg
    17881 cccgtaaagc aggcaaaata aaatgaggca ttctgcactg ttaattatca actctggctc
    17941 ctcaagtgtt aaatttcaag tattctccaa tacagagaaa cttgatcttc tcgccaaagg
    18001 caagattatt aatattggag gagaaccatc ttttaccgca gttaacgaaa catcggagca
    18061 ccagagaacc ctaatcactt tggaaaagga catgacgtat gagcaagcca ttcatcttat
    18121 aatggactgg attagtcaac agcagcaaaa ctgggaaatt catgctgtcg cccatcgggt
    18181 cgttcatggc ggagaatatt tcactcaaag tgtcctggtt actccgcaaa tcatcagcca
    18241 attggaaaca ctgaattctc tggcgccctt acaccagcct cataatttgg ctgccataaa
    18301 aatcattggt cggattaagc caggattacc tcagattgca tgctttgata ccgcctttca
    18361 tgctcaacat actccattat ttactacttg cgctttgccc gaatatttaa gacaacaagg
    18421 gattaggcgt tatggatttc atggcctttc ctatgaatgg atttctctcg ttctcaaaaa
    18481 agactaccct gatttggcta acaatcgaat tattattgcc catttaggca acggcgccag
    18541 cttgtgcgct ataaataggg gaatcagtat agacaccacc atgagtttga cagcgctgga
    18601 tggtttacca atgggaactc gttgtggcag tttggatcct ggggctgtta tttacatgat
    18661 gagagaattg aatcttccac cagataaggt agaggaaata ctttataacg aatccggctt
    18721 gcttggttta tcaggaaaaa ccaataatgt gctggaatta caatccagcc ctgataaaaa
    18781 cgctcaattt gctttggaat atttttgcct gaaaacagct caatttattg gaatgatgac
    18841 catttcgcta ggtggagtag atggtattgt attcacagga ggaatagggg aaaattcaga
    18901 accgattaga gcaaatatcc tcaagaggat agagtgctta aacccttttt ctgttatcgt
    18961 tattcctgcg aatgaagaaa aaatgatggc tatgcatacc ttgacagccc tcaggacgta
    19021 aaggcgtatt ttttactggc ataggtaatg agtataaaaa atggataaag actactataa
    19081 aatcatggga gtcagtcagg atgcttctga aaaagacatc aaaatggctt accgaaaatt
    19141 agcccgcaag tatcaccccg atattagtaa agaaccagat gctgaagaac gatttaaaga
    19201 aatggcagaa gcctatgaag tactgaaaga ccctaaaaaa agatccgaat atgatcaata
    19261 tctgaaatac aaggaattca atccccaaag cgatggattt accgggcgaa gaactcaaga
    19321 acaacccttt caggaatttc attttgacag cgattttttt gaaaccttgt ttggacattc
    19381 gcgttatcaa cagcaaccga tgactggtca aaactatcat ggcaaaatca ctatcagtct
    19441 ggaggaagct tatcatggag ctgttaaaaa cttacaagtt cctgttgaac aaggcacaga
    19501 gactaaaata cagaccctga aagttaaaat ccctgcaggg gttaaatcag gacaacaaat
    19561 ccgcttggca ggacaaggag gcagtggaag cggaggcggg gctcgcggcg acctttatct
    19621 aacagtcgag gtgatgaaac accccatatt tgatgtcatg gagaatgata tttatctcac
    19681 attacctatc acaccgtggg aagctgcttt aggagctacg gttgtcatac caaccctggc
    19741 aggaaaaatc gatttaaaaa tccctccgag ctcacaaggc gggcaaaaac tcagaattaa
    19801 aaatcgcgga ttaccaggtt caccccctgg taatcaatat gttctattaa aaattattac
    19861 cccccctgct caaactgaag aagcaaaagc tttatataaa aaaatggcgg aggtaatgcc
    19921 ttacaaccct cgtaagacta tgggagtata agcatgagca aagacaatct tttaataggc
    19981 atgcttatcg aagaaaccac aacgatttct tttattgaag tatgccaaaa gtatcatatt
    20041 cctgaagaat tattcattga aatgatcgaa caaggtttgt ttccagatca accatctgat
    20101 cccgaaaaaa ttgcattagg ccaaaaagaa ttgcgtcgcc ttgaatccgc ctttcgatta
    20161 catagagatt taggcattaa cctgcctgga gtcgctttag ctcttgattt actggaaaaa
    20221 attgatcaaa tgcatcatga gcttgaaatt ttacgcaagc atttttaggc atcagaatct
    20281 ttaggattat aatcaggtat ctctttaggg cggtaacctt taatcgttct aaaaaactcg
    20341 ataatgtact gaaaatcctt tttaatatct cctgtggtcc aaaaaggttc atggatacgg
    20401 atttctttag tggaataatc gggccccacc atcactatag gaatacctgc ttgccgagca
    20461 atatgataaa acccctcttt ccatctggtc accggacttc gggtaccttc gggagccagg
    20521 cccagcttta attcatcgcg ttggcgaaac aactctacaa ctttctcaac cctgttcccc
    20581 tttttagaac gatcaaccgg ggtgcctcca actgcacgca aaaaatagct caaagggaaa
    20641 aaaaataact gatgtttcgc taaaaaattg attttaaccc cgacaacaaa acgggcacac
    20701 aggcaaatga caaaatccca attgctggta tgcggagcaa caatcacaat atattttttt
    20761 tcattcggta attcaccaac tatcttccaa ccgattaatt taaaaataaa acgacatatt
    20821 ttttgcatga caaaaatcca tttttgtttt aaccaaagta tacttgattt tgatatttat
    20881 ttagaagaaa ttgttatttc acatgatcaa ggtacaatat cccttttttc gaaaccactt
    20941 accctatgct ctatcatgat ccattgattt cagaacacac catgaaatgg gtgcggacat
    21001 tcattattaa ttacacgatt tgcccttttg caaaaggccc cgtaaacaaa ggcactttaa
    21061 gaataattac ttcagatacc ctcaaaaaac accaggcact taaagattta atcactgaaa
    21121 ttcaatttct ggaagcaaac cctgctgttg aaacgactct gcttattttt gctcgagcat
    21181 ttaaagactt tttttcttat ctggatttca taagccttgc tgaacaatta attcaaaact
    21241 taaattatga agggatatac caactcgcta ctttccaccc ggattattat tttgctgata
    21301 cttctccaga tgatgttgcc aactacacta atcgttcccc ttatcccatg atccatttat
    21361 taagggaaga cagcctggac aaagcaattg ctgcctatgg tgacacccac attataccag
    21421 aaaaaaatat aacgactatg aatgagcttg gtttagaaaa aatgaaacag ttattggctt
    21481 ccatttctat accctagtat gcaacaggac ttctgaaaag taccttgctt tataattcat
    21541 tcttataaat gaccccatct ttcataatca atttaaggtt ttcatccggg cggagcagaa
    21601 catttaaatt gactaaagga ttaccatcta ccactaacag atcagcataa gctccttcgc
    21661 ttatgacgcc aagtttcccc ttttgcatta ataattccgc attgactacg gtagctgaac
    21721 gaatgatatt ggctgctgaa tcaagctcct tgcgcatttc aagttctctt aactgatctc
    21781 tctgtgcctc aggaccccac aaatccgtac caaaagcaat aggaatctta tattttaagg
    21841 catattcata aacctttttg cctttttcaa tagtattttt caaattggct acaatagcct
    21901 cagacaaccg gttttgttca gcactttcaa ttctttgcgc caatgaaata aatgtcggat
    21961 cgtaaaacac accactttcc gacattaatt ctacagttgg ttcacttaca aaattagcgt
    22021 gttcaatgga acgcacaccc gatttaatgc actttcttac tgattcatcg ctataagcat
    22081 gagccataac ataagtattt cttgctcgtg cctcttcaac aatagtcgat aactcctcct
    22141 cggaatattc atacagtgtt ggattgctaa gggaagggaa aacaaccccg cctgaagcaa
    22201 atacttttaa ttgtgttgct cctttcctta actcttctcg agcggccttt ctcacttcat
    22261 caaccccatc tgcaataacc gacatcgtcg agaaagaact aacatgccca caaggatcaa
    22321 tctgttcatt cggttttcta aagtcagctc ctccacccgt ttgagttaat gccctcccgg
    22381 aataaaataa tcgaggccct ggaatactct tattatccag taattgcgct attctaaaat
    22441 cagcgcctcc agcttctcgt aaagtcgtaa aaccataaaa caagctgttt ttaagataag
    22501 tagcggcagc caaaaatgtc tctgcttcgg aatagaagat attttttggg cttagggtca
    22561 atccggtaac atgagcatga gcatcgatta aaccaggcat taatgtcttc cctttgacat
    22621 caatcactcg cattccttcg agaggttgaa gcggtgtttg cgacacctgg taaatcaaat
    22681 cattttgtac cataacatca aaattttttt gaatctctgt agattctctc aaaataacgt
    22741 tagcattttt aaaaagaatt gagttcattt taatctcgaa gtcattaatg taatttactc
    22801 tatttatagt ctattttggc aactccgact cgctctggtt ttccttaccc tgctttttca
    22861 aaagtattta atttaacagg gccccataga aaattactaa aacccgagtc cacattattg
    22921 aaataatgta ctaaaattta tcatatagaa ctaattatca ggtgcatcat gaacacacaa
    22981 gatccggcac tttcgctctc aaatccacgg ctgttatcag caatctattt tggcttgtta
    23041 tcggtcgttg gcacgatact gatcaatgca ttcctcactt caattggaat tgaagaaatt
    23101 atcccggtct atcaatcggt cattcttgga atggttgtag cttcctgcac cggggccatt
    23161 tttggggaag ccatcgttca ctgtaaaaaa ccttataaaa gaaaaacctt ttggctagga
    23221 ttcagtatgg tcatagcctc actacctgtc tttgatctcg gacttgtctt cttgatgtca
    23281 gaagatcatg ccaagctttt tgaagtaacc actttaacaa gtatggtttt gttctaccta
    23341 gttgttctag cctatagtta tgtgcttttt ggtattttgc tggctgttgc atccgggcta
    23401 gctgctatgt atttaagagg gcaattagta tacgatatcc tgcataccca aacccgacgt
    23461 caacaaaaac ataagcatgc attggcaaaa agtaaatcag aaggacatgc ccataaagtt
    23521 caccgatgaa cttaagtcag atgagcttta taatctattg tctcaaggag cggtcgttat
    23581 tactcctaac aatagattaa gcgggaccat ccttcagaat tacttcactt attgttccag
    23641 aaaaacaatt accaagccaa tatgcttgcc ttttaataca tttctggtta actcttttga
    23701 acaactcaaa ttccaaaaac cagacctgga acatcccata cttttaaact cggcacaatg
    23761 ccaacattta tggcgaaaac taattaaatc tcatccttct atcatttaca cggaaggctt
    23821 gcttaaaaca ataatgcaag catgggaata ctgtgagcaa tggcaactta cacctgaagc
    23881 gcctgctttt cactacacgc cgcaaacaca acaatttcaa caatggtggc aatcttttaa
    23941 taaaatactt aaagaaattt ctgccatcca tcagcatcaa ttaattccct acttgcttga
    24001 ttccaattat caagggctcg caaaaaccgt tgtctggatg tgctttgatg actttaatcc
    24061 acagcaatta tctctgcaaa attatttgaa cacgcagggg gtaattcaat atggttatga
    24121 tttaaaagaa aaccaggaga ctccggaggt tttctcagct aaagacaaaa aaacagaata
    24181 ccaggaattg atgacctggt tggagaagaa aattcaacaa ggaaaaaagc aaatcggggt
    24241 tgttgtgcct aaccttgaac aagaatcatc ggttttgcaa cgaattttac gcgactgctt
    24301 tgaacccaat ttatttaata tttctttagg caaaccatta agcgcatttc acttgatctc
    24361 tcatgccctc atttggctga gtcttgatca caatctctta aataatcatc aagcggcttt
    24421 actgctccaa tcaccctacc ttggcaaggc aaaagatgag tttcttcaaa gatcagatta
    24481 cttacaggaa agcaagttgt tgcaagacca atctttcccc acaaaattat ttattgagga
    24541 cattgcctac cacacgccta aactggctga attgttaaaa aacatgaatt tttatccgga
    24601 aaaaactacc ctggatgagt ggattgagtt atttcaggag aggttaaatg gcattggttt
    24661 tcctggagat tatgttttga actcagagga ttatcaatgc tttaatcgct ttacaatgat
    24721 ttttgacgaa ttcagacaat taagtctgat aagcagccat ttgactaaaa aagaggcatt
    24781 tgaaacgtta aaacaactca tagacaacac tatttttcaa ccacaaaaaa gcaattcacc
    24841 aattcaaatt ttgggattgt tagaggcatc aggttgcgaa tttgacagcc tttgggtaat
    24901 gggattgact gatcgatgtt tacctcaaca aacacgttta tcgcccttta tcccatttga
    24961 attacagcgt gaactgggca tgcctcacag tatcccggca agagagctcc aatatgccaa
    25021 gcaaattttg caaagactgc aacgaggcag catggaaacc gtttttagtt atccacaatt
    25081 tgacgatgac acgcctaatt taccctgctc attgattact cattatcctc cctacctcag
    25141 ccggtctgct gacaataaaa caaatgttca aagttgtttg gttcctcttg aagaaaacta
    25201 tgaagtacct gtaaaacctg gtgaatccat ttctggtgga accagcttac taagcaatca
    25261 ggccaaatgc ccttttaaag cattagcaca acaccgactt aaggcaaacc ctgttctaaa
    25321 aactacagat ggaatggatg acaaggaaaa aggtcaatta atccataaaa ttctggagtt
    25381 gctctggcaa agattggaaa atcaggaaca cttaattaaa ttaaaacccg ctgaacttga
    25441 acgatttatt gatgaagcaa ttcaaggcgc tttaaatccg attaggcaac gacactccca
    25501 agcttttcct gacttgattg ccaatgttga atacatgaga ttaaaacgaa ttatcttgtc
    25561 ttatctggaa tgggaaaagc aacgccctcc ttttaaaatc acaggcctgg aagaaaatca
    25621 ttcgatcaat ttggctgggc tcgattttaa agtaagagtg gacagactgg atgaagtagg
    25681 taacaaacaa tgggtcattg attacaaaag cactctccct ggcggcaaac catggaatga
    25741 agaaagacca atggagcctc aattgctact ttatgcgttg cttaacgaac aaattaatac
    25801 tttgttattg attcaattaa aaaccggaaa aatcaagctc agcggtttta gcgaagaaaa
    25861 atgggaaacc aaagagatta atactttgaa agaggatgaa aattgggaag actatcgaca
    25921 acgctggcta gaacaattaa ctcatttagc tgaagaaatt aagtctggct attgtccgcc
    25981 acgccctcaa aatctgacca tttgccaacg ctgtgatttt cagaacttat gtcgcttcca
    26041 ggctaaacaa taaaccagtt ttaatttatc gggactactt atgcatcatt taattcttgg
    26101 ttatggctat tgcggttatt accttgccca agagcttctt aggcatcatc aacaggtaac
    26161 cgtagtatca cgacaattaa aaaaggaatt ggaactttca aaactacagc atataagcca
    26221 tgatctcgaa tacccatttc attggagcga gcccgactcc attatctact atctaatccc
    26281 cccgccttct caaggtacct gcgatacctt cttgcaacag tttttaaatc aaagttcatt
    26341 aaatgcaaag aaaattattt actttggttc aagtggagtc tatggaaacc atcaaggagc
    26401 ctgggttgac gaattatcag cttgtattat tgactcccca cgtcaacaaa gacgattgga
    26461 tgcagaacaa caatggctgg cctattgtca gcaacacaat aaagagcttt ccttactgcg
    26521 tattgcaggc atctacgggc cgaacagaat cccagccgat gcagccaaat cgaaaatccc
    26581 aataataaaa ccgactgaag ccccttttac caatcatatt tttgtcaagg atttgtcttt
    26641 aatagcttat ttgcttggcc aaaagcaaac aacatcggct gtctataatg ttgccgatgg
    26701 caacccaacc catatgggcg aactacaaca aacggtagca aacgcattag gaattgaatc
    26761 agcatcctat gagtcctttg agcaagcttg ggaaaaagca agccctatga aaagagagtt
    26821 catgcgagcg tccaaacgat tgaagattga tctgttaaaa actgcattgg gtgaagatat
    26881 tcagcttacc tgtttgtatg atgcagtaaa acaaagctta tgattttctg tttttgtaaa
    26941 aaactattct tgctaagtta aactttaatt ttctttaaaa tatggtatta tgctgccaca
    27001 ttggttatat ataattcaat aattcgttat aaatcaatag gattttaaat ttataattac
    27061 aattgaggta aactatgcct tcttacaaca atgcccggct cttggaaaaa cgcttgcaaa
    27121 attgtgatac acaattaaat caatttttta atggtcagga attatcaata aagtttctcc
    27181 aacaattaaa tcaaatcaag tatttttata attcagcttt cagcaagacc gaaaatgaag
    27241 acgatggcct agaagtcgtt gaggagtacg aaaatttcat ttcgttggtc tcacaggtaa
    27301 aatctggaca aattaatgct gacaaagcgt ttgaaacaat aaaagatacg acagaatcca
    27361 ggcaggcaga cgttattatt gctaatttct ttaaagtttg tgaactcatg ttctgggcta
    27421 ctgcagcatt ttttagttat ctagcctgtg tatcagtagg tatcccctta actttttgtg
    27481 atcctctcct gggtatcgca gtctccttat ctaccttttt gatgacaatt agcacagcaa
    27541 gcaaattttt agactgtttt gatgagttcc aatcgttaga taagattaac gagcatgata
    27601 aaaatcaaaa ggataccgta cggtttttca gtaaaacttc aacctctatt tcatcagtac
    27661 gcaatgaaga agctgaagta gaagaaagct ctcagagatt atatccgaat ttacaagcca
    27721 tctaaattaa taaacaatgc cacatcagac caaagattat tttggtctga tgttttggtt
    27781 tgctttcaaa gagcataatc aaaattaagg caatactgag tcaactcata tttaaatgtt
    27841 ctctaaacag ccgttccgat aggaggctac tctgttacaa attaatctct gaaatctctc
    27901 tattcatcct taatcaccaa ataaatcata acggctaaaa tagtaatgaa gatatgaaaa
    27961 gctgtggtaa gggctgagtt gagcatggga gacatccaca taccaaacca ctctcctcct
    28021 atggacataa aaatcacttg ccaggttaca aaacccaacg taagacctcc aatagcccag
    28081 ttcttggaat gattaaaaac agaagcactt tcatttcttg ccttaaacaa tttccacgca
    28141 ccaattccgc aaaatatggc ggttaataat tccaggataa caatgataat aaatgcaaca
    28201 taatgcagcg ctggactagt aattgctcga taggtgatgg tggaattagg aaatatatcg
    28261 ctcatggtta atacttttac cacagcagga aaattggcag aataatcagt gatgtttcca
    28321 aatgcagtaa gtaaacaaaa aaaagaaaca gcaacaacaa gcaacacttt tgagaatctg
    28381 ataatcatca tttcatcctt tgaaacaagt taaaaaatat gtatcaccta attgataaga
    28441 gcgttcgtgg agaggatgct tatgctcgtt tctcaccaag ccaggttcgc tttcttcaag
    28501 cttcgctaac caaatcgatg aatcaaaaaa tcttagccca aacctttgcc cactttatca
    28561 tcaactaccc tttctctttg agtatttctg gcaaaacgta acaataaatg attcgctctg
    28621 atcgtcgcct cgttcagagc ttcctccgga ctcatcaaat ctgcaaacat cgcctctaaa
    28681 acttcatcat taattccacg aatttgattt tgtgggccca tgtgtttata aggtttctca
    28741 gataaattgt cctcgagatc tgttctggct aataataaag cgggatgttg acttgattgc
    28801 acaatgttag cgtaaattcc ttttaacccc aacggcaaat agcccgtatg ctcatgccag
    28861 cgcttttgca cttccggttt tgcaataaat gcaaaaaatc tggcaattcc tcgatattgc
    28921 tcttctgttt gtcctcctac agcccataga gcagcacccc ctgccacatt cgcatgtctt
    28981 atcggactgg cccctgtatc caaaggcatt gttgcaacac ccaaatgaaa aggaacaagg
    29041 gcagacaaac tattataagc acccgatgat tgactaaata aaggacaaac actactggta
    29101 aataaaatgg tagcatcatc aacacgacca ccataacgaa aataatgcaa atcatgccag
    29161 cgctttagtc gttgaaaatg agccattaat tggggagtat ggaaagcagc acgagcagga
    29221 ttaccttgag ttatgggcaa gccatggata gcaagaaaag attcaaataa cacccatcca
    29281 ggataagccg aggtataagt acagtcataa cccgcttttt taattttttc tgccattatc
    29341 tccattgcag accaggtttg agggaagtta tgcttgccat atcccacctt ggctaaaatg
    29401 tcagcattat agtacagaac tggtgccgag agattgaaag gcatcgccat taattgccca
    29461 tccctgctat aaaactcacg cactgattga ataaaatcat ccttaggcaa actgatcccc
    29521 tgctcactca tcaacaaatc aacgggtttg atcacaccag ggggagctag cataattgaa
    29581 gtgcctactt caaaaatttg aactattgat ggtgcttgac gagctctgaa tgcagcagca
    29641 aaattggtaa gagtttccgt ataatttcct ttgtaaatcg gtttgactcg atattgattc
    29701 tgacttttat taaaatcatc ggcaagcaaa cggacttcat cacccaaatg ccctgccatc
    29761 gcatgccaaa aaaccagctc aactggtttg gcttgagcag gaatgacact cagaaaaatt
    29821 aacaaaagca aaaagagtat tttcatttta acaagtctgg ataatcacta aagagagcat
    29881 caacacccca atcaaataac tttttggcca aacgtttacg gtttacagta taggcacata
    29941 ctacatagcc ttgatcctta actgccttca ctcgatcagc ggttaatatc tttctattga
    30001 aatgtattga ataacattcc aattgtttcg ccttctgtaa ccaatcttta tcccactcat
    30061 gcagcaagag ccctaaaggc atttctggag caatactgcg acataacacc aaagcatccc
    30121 attcaaaaca ggaaacgaga ggtaattctt tattttgggg ccaatgccga tggatataac
    30181 ttagcactga tacagtagtt tgctcaactg ttccaggata aggttttatt tctatgttcg
    30241 cctggacatc ggaaaaagac aaccacttta atacgtcagt aaagtgtgga attttttctc
    30301 ccttatactg cttcgaatac caagagccag catcaagacc acggagataa tctgcctcca
    30361 ccagtcccac ctctcctgtt ccattcgtgg ttcgtttcaa attgtcatcg tgaatgataa
    30421 aaggttcacc atcaagactg cacatcacat caaactcaat gaaacggcaa cctaaagaca
    30481 atgctttatt gaaagacgcc atcgtatttt ctggagcata acccgatgct ccccggtggc
    30541 cgataatttt ttcaaggatc aactaattaa tctccctgtt tgcgattgga tgctgcttct
    30601 accatagcgg tcaatattaa ttcattactc actgtcaatg gtgccggagc agcagccatc
    30661 gctaaagcat tcatttcttt cgcttgatag gctcttggtt gctggggaat gttgtcaccc
    30721 tcgaaaaaaa ccaggttact cacagagtaa ttttgcttgg gataggcttt attcatacga
    30781 tcaatttcat cattcacttg ctgataaagc tgttcacgaa tcttggcacg tacgacttga
    30841 gtctcttcca aactgggttt gaactccaca ccactgatct cgtacttggc tccaggctta
    30901 ctaaccgatt tggcattctg ataaatatta gtcagagcag attgatcgac tcttgcctga
    30961 gcttgtacat aaagtttctc aaggccagaa ctatcctgag aacggtcaaa ctgcaccaaa
    31021 tgccactccc ctttagcaat tttgtttaag ctatccatta tgtcagcacg tgctttcacc
    31081 aaatcagcat tacttagagt ggcattgata ctgacattga gtaacgctgt ttgagtagta
    31141 acccattttt tagcagaaac ttgaaaaaaa atcttatcaa gcaccaaatc tggtgtctca
    31201 ttatcagccc aaacaaaagg agttaagacc attaaaccca gtatccaggc aatttttctc
    31261 ataatatgct ccattcataa tgtttctatt ggaattagtg tataccagca agctagaaga
    31321 caccacagca gtttgaacat aatcactcta aaacaaatcc tatacggcat tgcccgcaaa
    31381 cagtcggatt tgagtgcagg ttgagaaaat aaacagagta aaaaagcgca gtgtacatta
    31441 aagtacatga gcatttttta gaatactttt tctcaagatg cgccaaatac ggcattacca
    31501 gcaaacggtc ggatttgagc gcaggttgag aaaataaaca gaataaaaaa gcgcagtgta
    31561 cattgaagta tatgagcatt ttttagaata ttttttctca agatgcgcca aatacggcat
    31621 taccagcaaa cggtcggatt tgagcgcagg ttgagaaaat aaacagaata aaaaagcgca
    31681 gtgtacattg aagtacatga gcatttttta gaatactttt tctcaagatg cgccaaatac
    31741 ggcattacca gcaaacggtc ggatttgagc gcaggttgag aaaataaaca gaataaaaaa
    31801 gcgcagtgta cattgaagta catgagcatt ttttagaatg tttttttctc aagatgcgcc
    31861 aaatacggcc gtttgctaag aattacattg atttaattga cgaatagatc tataacttct
    31921 cccaaaatta ttgcaagagg agcttaaaaa tgatgtctgt tgacaatatt aaaaatgata
    31981 accaggtttt ggctgaagaa gatttcctcg aatacgcatt gtctcatgga tttggtgatt
    32041 tacattttaa ggtggatccg caaacgggaa tgaaagctat gattgcaatt catagcacca
    32101 agttgggacc tgctttgggt ggttgtcgat ttattgaata tcctaatact gctgctgctt
    32161 tgaaggatgc tatgcgcctt gccagaggca tgagctttaa ggctgcttca gtcaatttac
    32221 ctttgggcgg tggaaaatcg gttattatta agcctaaggg gttttttaat cgaacggaat
    32281 acatgcatcg gtttggtgaa ttcgttaatg aattgaatgg caggtacatc actgctttgg
    32341 atagcggtac tgaattaagt gacatggata ttattgctga gcatactgac tatgttgcca
    32401 gtttgtccag atacaatgga gatccttcac cttctactgc taagggtgtg ttaagaggga
    32461 ttcaggctgc tgtcgccttt aaattaggta aagactctct caaaggtttg catattgcta
    32521 ttcagggtct tggccacgtc ggttatgctc tggcaaaaca attacacgaa ttgggcgcga
    32581 cattaactgt tgctgatatt tcacctgctg ctgtgaacat ggcggttcaa gagttgggag
    32641 cgaaagcggt cggcactgac attatccata aagtaccgtg tgatgtattt gctccttgtg
    32701 ctttaggtgc gattatcaat gatcttacta ttccccagtt acaaaccagt attattgctg
    32761 gcgctgccaa taatcagctc gcgcacactt accatggcaa gattttacac gaaaaaggca
    32821 ttctgtttgc tgtggattat gtgattaatg caggtggttt gatattcgct gcctctaaat
    32881 acttacaaac gcctgaagac aaggtaagtg agcaaattga tagtatctat acctctctga
    32941 ctgaaatttt tactcgctct gctaaagata atttacctac tagcgagatt gctgacactt
    33001 tggctaagga aaaattggct taatttggat accgtttatg aaacatttat cccttactcc
    33061 tgagttgtac gaatacctgc ttgatatttc cttgcgtgaa catcctgctc ttgcggcatt
    33121 acgcaaagaa acatccacta tggaactcgc caatatgcag gtagctcctg agcaagccca
    33181 atttatgcag atgctgatac gtttgactcg tgcaaagagg gtattagaac ttgggacatt
    33241 cacaggttat agtgccttgg ccatgtccct tgctttgcct gatgatgggc aagttatcac
    33301 ctgtgatatt aatgagggat ggacaaagca tgcccatcct tattggcgtg aagccaaaca
    33361 ggaacataaa attaaattac gccttggacc tgctcttgac actttacatt cattacttaa
    33421 tgaaagtgga gagcatcaat ttgatttcat ttttattgat gctgacaaaa ctaattatct
    33481 gaattactat gaactggctt taaaattagt tacacctaaa ggattaattg ccatagataa
    33541 tattttttgg gatggaaaag taattgatcc gaatgatacg agtggacaaa ccagagaaat
    33601 taaaaaatta aatcaagtga ttaaaaacga ttcaagggtc tttgtcagtt tgttagctat
    33661 agctgatggg atgtttctgg tgcaacccat ttaatcttta aaatccccgg caagcaatac
    33721 actgcattgc tatgtaaata agtacaagga gaatctgata atgcaaaata acaacccctg
    33781 cggattagat ggctttgcct ttttagagtt ttcaggccct gataggaata aattacatca
    33841 acaattttct gagatggggt ttcaggccgt tgcccaccat aaaaatcaag acattactct
    33901 tttcaaacaa ggggaaatac aatttatagt gaatgcggct tcccattgtc aggcagaagc
    33961 gcatgcttca actcatggtc caggcgcttg tgcaatgggc tttaaagtaa aagatgccaa
    34021 agtcgctttt caacacgcta tcgcgcatgg cggtatagca tttcaggatg cacctcatgc
    34081 caatcacggc ttgccagcca tccaggctat tggtggtagt gttatttatt ttgtcgatga
    34141 agaacaccaa cccttctctc atgaatggaa tattacctca ccagaacccg tagttggaaa
    34201 tggcctgact gcaatcgacc atctcaccca taacgtttat cgcggtaata tggataaatg
    34261 ggccagtttt tatgcttcca tttttaactt ccaggaaatt cgttttttca atatcaaagg
    34321 aaaaatgact ggtttggtca gtcgagcatt aggtagccct tgtggcaaaa tcaaaattcc
    34381 tttaaacgaa tccaaagatg atttatcaca aattgaagag tttcttcatg aatatcatgg
    34441 cgagggcatt caacacatcg ctctcaatac caatgatatt tataaaacag tcaacggctt
    34501 aagaaaacaa ggggtcaaat tcctggatgt gccggatact tactatgaga tgattaatga
    34561 ccgcctccca tggcacaagg agccactgaa tcaactccat gctgagaaaa ttttaattga
    34621 tggagaagca gatcccaaag acggcttgtt actgcaaata tttactgaaa acatatttgg
    34681 accagtcttt tttgaaatta ttcaacgtaa aggtaatcag ggatttggcg aagggaattt
    34741 ccaggctcta ttcgaagcta ttgaaagaga tcaagttcga cgtggtactt taaaagaatt
    34801 aacctaggca aacagttgaa atgtaatgat gctgtttttc aggcataagc caggagatct
    34861 atatgaaact cgccagtttg aaatcaactc gttctcggga tggagagctt tgcattgtca
    34921 acagattgct gaccgctgca gtacgcgttc ctcatatcgc atcaacactt caatatgccc
    34981 tggatcattg ggatgttgtt gaacccaaat tacaactggt atatcaacag ttaaatgata
    35041 atcaaatcga agaggccttt gcgtttgctc ccgagctatg tgcctcgcca ttaccacgtg
    35101 cttatcaatg ggccgatggc agtgcctatg taaaccatgt cgaattggtt cgtaaagcca
    35161 gaggagcaga aatgcctgct gatttttgga ccaatccact catgtatcaa ggcggatccg
    35221 atgcttttct ggggcctcgt gatcctattt tggtaagctc agaagcctat gggattgact
    35281 ttgaatccga ggtggctgtt atcactgatg atgttcccat ggggattgat catacaatcg
    35341 ctgcccatca tatcaaactg ttgatgttgg ttaatgatgt ctctctacgc aatcttatac
    35401 ccgaggaact ggcaaaaggg tttgggttct tccaatcaaa acctgccagc agcttctccc
    35461 cggtagcgat aaccccggat gagctaggtt cttactggga tggacaacgt gtgaatttgc
    35521 cattaattag tcattttaat gggcaattat ttggccaacc caatgctgga attgatatga
    35581 ccttttcttt tccagaactg atacagcatg cggccaaaac aaggttttta actgcgggta
    35641 ctattatagg ttctggaaca gtatccaacc ttgaccgcag caaaggttct tcttgtattg
    35701 ctgaaaagcg aatgctggaa atgatagaat tcggacaggc aaaaacaccg tttatgcgat
    35761 ttggggaccg aattcgcata gaaatgctcg atggtcaagg gtattcccta tttggctcta
    35821 ttgaccaaat agtgacgaaa tacgagaagg attccttatg atattgtatg actatttccg
    35881 ctctaccgca tgctatcgag taagaatcac tcttaattta aaaaaaattg tttacgaaaa
    35941 aattgaagtg catctggtta ataatggagg cgaacagcat agccttcagt atcatcaaat
    36001 taaccctcaa gaacttgttc ccagcctcga catcaatgga catatcttga gccaatcaat
    36061 ggcaatcatt gattatctgg aagaagtcta cccggaaata ccattgcttc ctaaagatcc
    36121 ctttatgaaa gccaatctaa aatctctggc tttaattgtt gcttgtgatc tgcaccccct
    36181 caataattta agagtgttga atcgcttaaa ggagcaattt agagccagtc aagaacaggt
    36241 actggaatgg tatcatcatt ggttaaaaac aggctttgat gcttttgagg aaaaactggg
    36301 aactatgaag cgtgacaagc cagtctgcct tggaagtgag gttagtctgg cagacgtttg
    36361 tttgatcccc caggtttaca atgcccaacg atttcatttt gatatggcga gttacccaat
    36421 aatcaatcaa atcaatgagt attgtctaac cctgccggca ttccatgatg cggcaccaga
    36481 agccatttca tcttaaaacc atgctcttct caactctacc gtgatcacgc ctttaggggt
    36541 tttctccaac atactccaag ctgttttaca tcgttgcagg taaccaatta aaacattttg
    36601 attgctgcaa cggtacctta caggcattga atcatattgc tcgttagaat ttacagccaa
    36661 acagtactca ccattaacca attcaatagc ccagggaaaa gcgcgagaca tatctaaggt
    36721 cttatggtgt tcattattca aaggtatccc tacgttaatt tgtacgctat caccaaccca
    36781 tggagattgg gggcatacgg cctccatttt tttgctacca gctttcacaa aacaaggatc
    36841 aaataattgg tcttcagcct gacaacgcca tgcgtcttcg cgtataatga tgcgggattg
    36901 ttcgtaacat tggccagaca ttattttttt aatgacaggt ggtgcttgtt caaaaacatc
    36961 accaaaaggt ctgtataatt ttagaacagt atcgctagcg ctggctaaaa aagaaaaaaa
    37021 acataacaat acgagtaaaa ctctttgcag catggtgttc ccccacggat tttatttagt
    37081 gtatcatcac attttaattc agggtttagg aaatactgca aacgggagaa aatcaattcc
    37141 tcgaaggatt ggaatatttc ctaagccctg aatcaatcgt tttcgatttt ggagtatata
    37201 atgacagaag tctttactat aaaacaatgt ctcgacggcg aaatcagtat tgatgaaacc
    37261 gttacggtaa gaggatgggt gaaaactcga agagattcaa aagctggctt gtctttcatc
    37321 agtcttcatg atggctcttg cttttctccc atacaaatcg tcgctaccga tcagttatcc
    37381 aactaccaca aagaagtaac aaaattaact gctggctgtt caatgatagc gactggaaaa
    37441 ttggtggcat cgcaaggaaa agggcaattt tttgaaatcc aggctgaatc cattgaagtg
    37501 gttggatggg ttgaaaaccc agatacatat cctattcaag ccaaacgcca tactcttgag
    37561 tttttaagag aagtagctca tttgcgcccc agaaccaata ccataagcgc tgtcaccaga
    37621 gtgcgtcatt ctttggcgca agccattcac cgtttttatc atgaacaagg ttttttttgg
    37681 gtgcatacac ctatcattac tgccagtgac tgcgaggggg caggagaaat gttcagagta
    37741 tccactctgg atttattaaa tatccctaaa aatgataaag gacaaatcga tttcagtaag
    37801 gatttttttg gtagagaaac gttcttaact gtttctggcc aactaaatgt cgaagcttat
    37861 tgtatggcca tgtccaaggt ctacactttt ggaccaacat ttcgggctga aaattcgaat
    37921 acaagccgcc atttggctga attctggatg atagaacctg aaatcgcttt tgcaaatctg
    37981 gaagacattt gtaaactcag ccagaatatg ttgcgttatc tctgcaaaac agttcttgaa
    38041 gagcgtgccg atgatatgga ctttttcaat caatttgtcg cgccgggatg tatagaacgt
    38101 atggaacata ttgcggattc tgaatttgaa attatgacct ataccgatgc gataaaagca
    38161 cttgaggcga gtgaccaaaa atttgaattt ccagttagtt ggggattaga tctccagtca
    38221 gagcatgaac gttacctggc tgaagtgctt tgcaaaaagc ctgtcattgt gacaaattac
    38281 ccgcaagaaa ttaaggggtt ttacatgcgt cttaatgacg atgggaaaac ggtagccgcc
    38341 atggacgttt tagctccagg catcggagag attatcggcg gcagccaacg cgaagagcga
    38401 ttggaaatac tggatagacg catggacgaa tgcaacctga ataaagaaca ttaccaatgg
    38461 tatagagatc tgcgccgtta tggtactgtt cctcatgccg ggtttggctt gggctttgaa
    38521 cgtctgatta gctatgttac aggagtatcc aatgtgcgag atgtgatacc tttccctcgc
    38581 acaccaggtc acgcggatta ttaaattggt aaaggtctaa aagagtcatc atcaacttca
    38641 actgtttgtt gttggggttg gtgactctct ggcagcaatc gccgtctatt tcttggcgaa
    38701 aatatttttt ctaatatctc atcatctgca ttactcgcaa gcggtcctcc tgcatcaact
    38761 gtatcataat gttccctatt gaaatcaggt ccgggtagaa atcctttgtt acttgtctga
    38821 gctgaagaag gtatttttga attttcggtt gagtgaggat aaaaaataga aaaacttctt
    38881 ctatgtgact ttacaggtag actacgctcg gattttctca atggttgggc ttttttttcg
    38941 tcctcaacag tgcttgtaat ttcattgatc tgaattgttt tatctttttt ttcatccatt
    39001 gttaacactc cttatcttat ttttattctc tcgcacacaa actgccttca tgcaaaacaa
    39061 gtactctatc cattcgttct gcaatccgtt gatcatgagt tactataacc agggctgtat
    39121 tcattttttt attaagttcc aacatcaaat caaaaacttt actggccgtt gcctcatcca
    39181 aatttccagt cggctcatca gctaggacac aatgtggctg atgcaccaag gctctcgcta
    39241 tggcaacccg ttgcctttct cctccagaca actgagcagg cttatgcgct aatcgtggtt
    39301 taagtcctac ctgttccagc atattaatag ctttttcttc tgcacctttc acagccatac
    39361 ccgccagtaa taacggcatc atcacatttt caagtgccgt aaactcgggt aacaaatggt
    39421 ggaattgata aacaaatcct aattgttgat tgcgcaattg acaacgttgt ttttcattta
    39481 ttttttgcca attgacattg ccaagagtaa tcaggccgct agttggttta tccagccccc
    39541 ccaacaaatg caaaagggta ctttttccag aacctgatgg cccgataata gcgatcctgt
    39601 ccccttttgt tatggccaag tcaactcctt ttaacacctc gactgtggaa gtaccatcat
    39661 gataggactt atacagtttt tgactggtta aaatgatgtc attcataatg taaagcctcc
    39721 gcgatgactg tctttgatgc tcgccaggca ggataaatgg ttgcagcaaa actcattaat
    39781 aatgccatgg cacaaacttg ccacagatca cgaaacataa ttttagaagg caagtaatca
    39841 acaaaataga tacttgaaga caacaccttc acctggaaaa aggactgcaa tgcattaact
    39901 atctccgttg cattattggc caacactaaa cctcctaaca acccgagaat tgtaccaacc
    39961 agaccaacca tcattccttg tacaataaaa acccataaaa tggtagaagg agtagcgcca
    40021 atagttctta aaatggcaat ttcagcttgt ttgtcattga ctaccattac cagagaggac
    40081 accaaattaa aagctgccac agcaataata agcaataaaa tcataaacat catcgttttt
    40141 tccattttaa ctgcttcaaa aaaagctcca aattgctggg tccaattgcc tacttgatat
    40201 ccttctccca aaagatcaga taattcataa gacaattcag gtgctttgta tacattatta
    40261 attttcattt ttatacctga aacgtcattc ttatccagtt gcattaattt ttgagcatct
    40321 tcaatattaa taaatgccaa tttagtatcg aaattaaatc ctgtaccagc agaaaaaaca
    40381 cctacaacgg taaagcgctt gaatcgaggg atcataccgg caggagttac tgttgcttgc
    40441 gggatcatta tcgtaacctt atccccaatc atcaccccaa ggctgtcagc aagacctttt
    40501 ccaagaataa tcccaaaatg tttcagatta tccatattac ctgccagtag tttcttattg
    40561 agatgagtta ctgattgttc tttttctggc agaataccgg tcaggacgat aggcaaaacc
    40621 tgaccttcat gagttaataa cccttgactt ccaacataag gtgcaattgc ctttattcca
    40681 ggaattgttt caaccttttt aaccacttca ggccaatcac ttaatcgctc atcaggacct
    40741 gttatggtga tttcaggcgc catcccaaaa aaacgacgat gaatttcctg atcaaaacca
    40801 ttcatcactg ataaaacagt gattaaaacc atcactccca tgcctatacc caacatggaa
    40861 cttagggaaa taaaggaaac aaagtgattt ttcttacgtg aacgcgtata acgtaatccg
    40921 ataaaaagag ccaatggttt atacataatc gtattcagct ggattaaagt tagtattgta
    40981 cagcacttat agaaaaggcc aagattgaag cctaaagatt ttaaagaact tattaataat
    41041 tcatcatcag acaacccaca tagttatcca cagaatctgt ggatatctaa taagtctcat
    41101 tcatcgatta gagcatcaat taagcaaata ccaactcaca taaaatgagc atccgttacg
    41161 atatagcaga tgctacacat aaccagaaaa ttattaaatg ggatgggtat ttgtcagtta
    41221 aattttaaaa ttaattagga aatgcattat ttaaatggag aaaagaagtt gttagggtgc
    41281 ttcgttttac gaagaataat gaaataacta aagggtttca ttggaagatg aaattgattc
    41341 aaggtaggat tatcattgcc ctctaaaatg gggattagtc aaatttggtg cccagggccg
    41401 gaatcgaacc ggcatggtgt taccaccgag ggattttaag tcccttgcgt ctacctgttt
    41461 cgccacctgg gcattcactg ttttttaatg gaggctgagg ccggaatcga accggcgtac
    41521 acggctttgc aggccgctgc atgaccactc tgccacacag cctcaaatct atatacttca
    41581 ctattgctct aaaaaaaaag cgacttcgat gtcgcttaaa tttggagcgg gaaacgagac
    41641 tcgaactcgc gaccctaacc ttggcaaggt tatgctctac caactgagct attcccgcgt
    41701 ctctacggga tgagattcta ccgaaaataa ataccctgtc aacagcaaaa ttaacttttt
    41761 ttcattaact ctggccaagc aatagccaaa taaatcacca tggaccaaat cgttaaaata
    41821 gcagacacat aaagaagaac aaaccctaag ctcccccacc aggaaacaag gggattaaat
    41881 gccagcaata ataccaaagc gatcatttgc aaagaagttt taactttccc tatatatcca
    41941 acagcaacac tagcacgact tccaacttca gccatccact cgcgcaaagc tgaaatcaca
    42001 atttctcgtc caacgattac gatagcaggt atagtgatat aatctatatc cttcgctcca
    42061 actaacaata acaagcttgt cgataccaat aatttatcgg cgacgggatc aagaaaagct
    42121 ccaaaatgag acatcatttc aagtttgcgc gccaaataac cgtctaacca atcagtaatg
    42181 cttgcaatag caaaaagaac agcagcggta ggatgagccc atttgaaagg aaaataaaat
    42241 accataataa atactggaat gatcgaaatc cgcattaagg ttaatatatt aggcaaactg
    42301 gttaacgtac tcactgctga acatccccct tttttatgct tccggcaaca tcagataagc
    42361 tgacaatagc ttataatcat caaatacagc caaagcacct gctgctttaa gcgcatcctc
    42421 ttgctgatgg taaaaatcca tgcctatagc attaacattg attctttttg ccatttccat
    42481 atccgttgta gaatcaccta tcatcaaagt tgatgaagga tctctcccga attcctccat
    42541 gatttcctca agcatttgcg gacaaggttt tgccggtact tgtccagcag agcgtgtcac
    42601 tttaaacaat tgatcaagct cagttgcctg aagagcacgg attagagaat tgtggccttt
    42661 atttgtagcg atggccaaat cgacctttgc ttgatgaagt tgttggataa attctaatac
    42721 tccaggtatt aaacgcactt ctgttggccg tgaaatcatt gcggattgca ctgcctgtaa
    42781 cagatcttcc tgctgcccgg cagttagatt gggcaatgtc ttttttactg cttgaaccaa
    42841 tcctaagtct acatattttc ttgcttgata tgggtcaata tcaccaaagc ccaacatttt
    42901 ggcttcagta gcaacagtat gtaaaataac tcccaatgta tcagcaatcg tgccttccca
    42961 atcaaaaatt actaattgat atgcactact catagctcaa taggctcctt gcacgcagtt
    43021 gctttaaagt atttccaaat ccatcgtcta catcagcttg aaatacatgc attaccccat
    43081 tcagattgaa ttgaatagac cttgcatgca gataaagacg attctgttga taatctatac
    43141 cttccacctc acccacctta gagccgtatt tcccatcacc aacaatgaca tgaccaaagt
    43201 aagcgctatg aacgcgaatt tgatgtgtcc gccctgtttt aggagaggct tctacccaac
    43261 aggcctgatg ataattttcc agcaatttaa aatctgtttc tgatgcctta ccatctttct
    43321 gaacggaaac aaccctttct ccggatttca atgtgttctt ctcaagagga gctgtaattg
    43381 tgattttctt tttaccgtcc caacgatgcg ttaataaagc ccaataaatt ttctgcactt
    43441 cgcgggcttc caatagcgct tgaatggctc gtaacgtgct tcttttttta gctaataaaa
    43501 gacaaccgga agtctctcta tccaatcgat gtaccaattc caaataggat aaatcgtgcc
    43561 ttgtctttct taatgcttca ataaccccca ggcttaaacc actaccacca tgtaccgcaa
    43621 tacccgctgg tttattaatt accaacaagg aagaatcttc aaaaatgata ctatccttca
    43681 agcgttttgc caaattatca cttacaaaaa tttctttaga atcagtcata cgaattggag
    43741 gtattcttat actgtcccca atttgtaaac gactgttagc ttgtgctctt tttttattaa
    43801 tccgaacctc gccgccacga ataatcctgt agatatggct tttaggaact ccttttaaaa
    43861 tgcgaatcag ataattatcc aaacgttggc cattctcttc ggagccaatt tctttataac
    43921 ttacttcact cattgtcaat aaacctgttt tctgagcgtg atttttttga tatgattaga
    43981 tatcgtacgg gaaaatccct tacg
//
LOCUS       DASLOE010000008        42939 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.008, whole genome shotgun sequence.
ACCESSION   DASLOE010000008 DASLOE010000000
VERSION     DASLOE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 42939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42939
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.008"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..599
                     /locus_tag="U6309_000337"
     CDS             <1..599
                     /locus_tag="U6309_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503796.1"
                     /translation="VLILFFSNSSLAKTINYPWTVSASLGYVNYQYVHEGDHITATGR
                     FAIGRDLLMKGPFLFGLELGLQSGNQMRLQMTEEEVDALGGLLVQTTLKPMVDALATI
                     RITPEFTAPLFLQLKAGTAYRQWQMDRDTINDISQLAGEIMAGCGYTITPNASITLSY
                     QGLFGDKPNFTIDLESGIGHVQKIPVQHAILLGIVLMI"
     gene            767..2149
                     /locus_tag="U6309_000338"
     CDS             767..2149
                     /locus_tag="U6309_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEN5503797.1"
                     /translation="MIHRNKVLNSILLILFTSQIAFAKSNPASQAYVDGKVSELKNEL
                     TNKINSIPSGPQGPKGDKGEAGPKGDRGEAGPQGLPGPKGDQGEAGPQGLPGPKGDQG
                     EAGPQGLPGPKGDQGEAGPQGLPGPKGDKGETGPQGLPGPKGDRGEAGSQGLPGPKGD
                     QGEAGPQGLPGPKGDQGEAGPQGLPGPKGDRGEAGPQGLPGPKGDRGEAGPQGLPGPK
                     GDQGEAGPQGLPGPKGDRGEAGPQGLPGPKGDRGEAGPQGVPGPKGDRGEAGPQGLPG
                     PKGDKGETGAVGPQGMPGPKGEAGDDGQGVPAGGETGQVLAKSNDLDFNTMWVDPANS
                     GIRRQLGDKALGGTVIYVNALGTHGLVVANSDQVNSSTWWDAQDSITNPAHFDNEGKL
                     YSDWRLPSRFELNLIYMMRDELGNFLAGNYWSSIEKSSANSWVFNSKTGEIKDIAKNK
                     TAAVRAVRAF"
     gene            complement(2173..4029)
                     /gene="uvrC"
                     /locus_tag="U6309_000339"
     CDS             complement(2173..4029)
                     /gene="uvrC"
                     /locus_tag="U6309_000339"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEN5503798.1"
                     /translation="MNDLQLSAELALFLTKLPSEPGIYRMLDEEGTVLYVGKAANLKK
                     RVNSYFSKQNTGVKTRALVSQIKSIEISVTRSETEALLLESNLIKALRPKYNVLLRDD
                     KSYPYIHLSNHPDFPRIELYRSKKKPPSGNFFGPYPGVAAVRETIVTIQKIFKIRNCR
                     DSYFKARSRPCLQYQIKRCTAPCVHYISPENYKLSVEDAIRFLQGKCQIILDELAERM
                     KQAVNQLNFEEAAVLRDQIKNLRLIQEQQGVVQLRGDADVIAIEVRPGFACIQCVTIR
                     EGQVLNSQSFFPTVPYAVLDEELDANSLWQQTFEAFVGFYYLDTSERIPDLIITNQSI
                     TESRSLEYILSQRRGKSCKIQVNPRGVKSRWMDFAVNNLRISVAEYVSKHSTIRSRYQ
                     ALKQLLALDKNIERMECFDISHTQGEATVASCVVFDTEGPRPSEYRRFNIEGITPGDD
                     YAAMEQAVTRRFKRLIDAQLLPDVLIIDGGKGQVSIVKRVLTSLGVEDITLLGVSKGP
                     SRKAGWEKLILVNENREFVLPEDSKALHLLQHIRDEAHRFAITAHRKKRQKTRVESTL
                     ESIEGVGAKRRQALLQRFGGLRELAKAPLEEICKVQGISEQLAKRIHEHFHP"
     gene            complement(4041..4700)
                     /gene="uvrY"
                     /locus_tag="U6309_000340"
     CDS             complement(4041..4700)
                     /gene="uvrY"
                     /locus_tag="U6309_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HEN5503799.1"
                     /translation="MIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVK
                     THSPDVVLLDMKMPGIDGWEVTRRLKKTNPNIKVIAVTAICSDPLPTRVLQLGAMGYL
                     TKESGAEEMAAAIRKVAKGEKYLSAEIAQKMAINSLQEPQDSPFDLLSEREMQVMLMI
                     TSGMNVQDIADRLFLSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQD
                     D"
     gene            complement(4825..5643)
                     /locus_tag="U6309_000341"
     CDS             complement(4825..5643)
                     /locus_tag="U6309_000341"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEN5503800.1"
                     /translation="MEFSSRYVAHVPDAQGLVDYSAQENRIWNILFERQLKLLPGRAC
                     DEFLSGLQTLGLNSSTIPQLPEVSERLKAKTGWQVAPVAALISAREFFELLAEKYFPA
                     ATFIRSEEELDYVQEPDIFHELFGHCPMLTDRVYAEFVHDYACKVLTFPEQDWPLLQR
                     MFWFTVEFGLIKTPKGLRAYGGGILSSISETVYCVESDIPVRILFDPVVAFRMPYRID
                     RLQPVYFVIDSYQNLYDFVLSDMGKFMDRARELGEFPPYFDVDPDNPNIHIRAC"
     gene            6087..7184
                     /locus_tag="U6309_000342"
     CDS             6087..7184
                     /locus_tag="U6309_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEN5503801.1"
                     /translation="MTIAILSTGDEIIHGDTLNTNAHYIAHALCSEGLPLGLQVACSD
                     KENEIIDSLNFLTEHHDILILIGGLGPTKDDITRFALAKFTKQPLIQHANALAHIEKK
                     ATIAKISLNQGNLQQCLFPANARLFPNPYGTAMGCSYSWKGKVFILLPGPPRECLPMF
                     NQDVLPLLLQTSRHSNKQILRWRIFGLAESEIAQILEEALQGLDCQTGYRLETPYLEF
                     KVRCKRELIEKITSIIDPILAPHIISSLETKASDQLRSLILKNNEPITIIDDVTGGLL
                     QSLLVRPENYHLLNFHNLNRTKLYFHCSGLEDYWSQQAPQGTTKLIIHYSNQMQEGRE
                     THQIPYRSAMVVHYAAEWLSFRLFHLINQLH"
     gene            complement(7143..8168)
                     /gene="obgE"
                     /locus_tag="U6309_000343"
     CDS             complement(7143..8168)
                     /gene="obgE"
                     /locus_tag="U6309_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEN5503802.1"
                     /translation="MKFVDEALIKVEAGKGGNGCLSFRREKFIPRGGPDGGDGGDGGS
                     IYFEASSDLNTLIDFRYTRQYKAENGQSGMGGNCTGKKGEDLIIKVPVGTMVYDADTG
                     ELLADISQPGVPVLIAQGGFHGLGNTRYKSSVNRSPRQTTPGSPGESRNLRLELRVLA
                     DVGLLGLPNAGKSTLIRAVSSSKAKVADYPFTTLHPGLGVVRVSPYKSFVMADIPGLI
                     EGAAQGAGLGHRFLKHLSRTCVLLHVIDIAPLDGSDPVADAKAILNELTQYNPDLLNK
                     PRWLVLNKIDMLPDEKEREEKIQSIIKGLKWKDKVFSISAIESKGTQELCYALMQLID
                     EMKESEA"
     gene            complement(8383..8661)
                     /gene="rpmA"
                     /locus_tag="U6309_000344"
     CDS             complement(8383..8661)
                     /gene="rpmA"
                     /locus_tag="U6309_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEN5503803.1"
                     /translation="MAHKKAGGSTRNGRDSNPKYLGVKRFGGQFVNAGEIIVRQRGTR
                     FHPGPGVGCGRDHTLYALVEGLVQFTTKGEKNRKYVTILPEQREEAAS"
     gene            complement(8674..8985)
                     /gene="rplU"
                     /locus_tag="U6309_000345"
     CDS             complement(8674..8985)
                     /gene="rplU"
                     /locus_tag="U6309_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEN5503804.1"
                     /translation="MYAVIKTGGKQYTVKEGDVLKIEMLPENVGNEIKFSEVLMLVDG
                     DKVTCGTPFVAKATVKAEVLDHGRHKKVKIIKFRRRKHHMKQMGHRQYYSQVKITAIG
                     K"
     gene            9228..9887
                     /locus_tag="U6309_000346"
     CDS             9228..9887
                     /locus_tag="U6309_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEN5503805.1"
                     /translation="MSTIQLEAQSRTDMGKGASRRLRRLENKVPAVIYGGSKKPMAIH
                     FSHNKVIKALETESIYSSVFDITVDGKVEHVILKALQRHPYKPIVLHMDLQRVSSKDI
                     LVKLVPVHFINEEQSPGIKAGGIVQHTMTQVEIRCQAKDLPEFIEVDMSKVGMDDVVH
                     LSDLKLPKGVQLTVDVADGSHDAPVVSIHAAKVSSTELEETPEVPASAVPTTDQGESA
                     E"
     gene            10006..10575
                     /gene="pth"
                     /locus_tag="U6309_000347"
     CDS             10006..10575
                     /gene="pth"
                     /locus_tag="U6309_000347"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEN5503806.1"
                     /translation="MAIKLIVGLRNPGSAYEQTRHNAGAWLVTALAQRHNSHFKIDKK
                     MQAELTEIDINNHPCRLVLPLTFMNHSGQTTRIISQFYKIEPGEILIVHDELDLPVGR
                     IKLKTGGGHGGHNGLRDITAQLGTGEFHRLRIGIGHPGHKDLVHQYVLSRPSMHDRQQ
                     IYDAIDRGIAIIPIVLSGDMARAMNQVNA"
     gene            10594..11685
                     /gene="ychF"
                     /locus_tag="U6309_000348"
     CDS             10594..11685
                     /gene="ychF"
                     /locus_tag="U6309_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEN5503807.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNVGIV
                     TVPDSRLDNLSDIVKPQQVIHATMQFVDIAGLVKGASSGEGLGNQFLANIRETDAIAH
                     VVRCFDNSDVVHVEGRVDPLSDIEVINTELALADMETLEKSLLKVGKNSKSGNKEAIF
                     ELKTLEKIKAHLDAGYPVRTLELTPEEEQVSKRLFLLTAKPVLYIANVDDNGYENNPL
                     LAKVQALASQENASIVALCAATEAELVELDEEDRQEFMVDLGLSEPGLNKVIRAGYEL
                     LGLQTYFTAGVKEVRAWTIRKGATAPQAAGVIHTDFEKGFIRAEVISYDDFIRFGGEQ
                     GAKEAGKLRLEGKEYIVCDGDVMHFRFNV"
     gene            12044..13159
                     /locus_tag="U6309_000349"
     CDS             12044..13159
                     /locus_tag="U6309_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEN5503808.1"
                     /translation="MSLLKKTREQDYSILVTTQDQQRKRHLLNVISIELLILGLLLII
                     TNLCLLRAWLIISMLIVGISILLGNLILIKKSFNLALSGHILNILVLSIIIGGNLIVG
                     KTTMSYLGWFYVSPIVAFATLGLAGLVIYGLLAIGAMFLFVFEFYIPIYSISQEYVFF
                     LDNTNHLFIFALIFTVLYHFLVQNSQYELLLKEQNYLLIADKEKFHYLSHHDSLTNLP
                     NRSYFHAHLQTLLENTDTTKEAITLYFMDLDKFKPINDEYGHEFGDQLLLLTSKRLQS
                     CFRKNDFIARLGGDEFTAIIVHKPHDQIAEALTLRIEQEFEQPFVINGQTIHCTISVG
                     IANYPRNAKNAEDLLKWADKAMYDNKRKKYQTLKQTS"
     gene            13293..14261
                     /locus_tag="U6309_000350"
     CDS             13293..14261
                     /locus_tag="U6309_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN5503809.1"
                     /translation="MTIDRIRALVSEDFNAVNNLIIDKIQSQVGLIQDLSQHIVESGG
                     KRLRPLVVLLASNACGYKGKDHISLAAMVEFFHTATLLHDDVIDESTLRRGRQTANSI
                     WGSKASVLVGDYLFTQSVNLMVEVGIKEILNLLANTSHQITCGEVKQLANRHNPALTL
                     DDYFDVIRAKTALLFAAAACIGPILTNAPIEIRNSLYNYGLHLGNAFQLIDDALDYCS
                     DAATLGKNIGDDLADGKATLPLIHALQNGNATQQQQIRESLMKGSLSHLPDILIAIEQ
                     TEAIPFTKRIAAQEVDSALSALEILPDSVYKEALVDLAKFALERNY"
     gene            complement(14288..14503)
                     /locus_tag="U6309_000351"
     CDS             complement(14288..14503)
                     /locus_tag="U6309_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503810.1"
                     /translation="MLLQIRDFIYREGVVSTQQLSREFHLDLTALQPMLDLWVRKGAI
                     RKCQGGANCKTACFKCHTPPDYYQSVV"
     gene            complement(14484..16739)
                     /gene="feoB"
                     /locus_tag="U6309_000352"
     CDS             complement(14484..16739)
                     /gene="feoB"
                     /locus_tag="U6309_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HEN5503811.1"
                     /translation="MTHALLIGNPNCGKTTLFNALTNANQRVGNWPGVTVEKKTGEFL
                     LGEHLIEITDLPGVYSLVANAEGISQDEQIAAQSVIDLEYDCIINVIDACHLERHLYL
                     TSQLFELGKPVVVALNMMDIAEHRGISIDTEKLESLLGCSVIPIQAHKNIGIPALQQS
                     LLHCSQKIKPLKLSLSVAAQQILNDLENQLLAKGYKNSFAYYFSRRLAEGDTLIGEKA
                     FTENLLINLQETDQNLDVLLADARYQKIHEIVTLVQKKHSDASEHFTAKLDKLVLHRF
                     LALPIFFAMMYLMFLFAINIGGAFQDFFDISTETIFVQGSGWLLQQLHAPNWVIALVA
                     NGVGKGINTTITFIPVIAAMFFFLSLLETSGYMARAAFVVDKAMRAMGLPGKSFVPMI
                     VGFGCNVPAIMAARTLDSERDRLLTVMMSPFMSCSARLAIYAVFVAAFFPSGGHNVVF
                     SLYLIGILMAVFTGYILRKSTLKGHASPLILELPAYHRPSLRRLLRETTMRLRFFVYR
                     AGKLIIPICVILGGLNAITWGGGISSGEANTDSLLSIIGQWITPLFAPMGIHQDNWPA
                     TVGLLTGMLAKEVVVGTLNSLYAQVGHVGEIAAAHFDFWGGIKAAFGSIPANLSELGS
                     ALWNPVSASAADSELSQSVYGIMSRRFDGAVGAYAYLLFVLLYIPCVSTMAVIRQEAN
                     KRFMWTSIVWSFVVAYATSVVFYQGAKFLDHPQQSMVWILAMSLSLLFVLAVFRYSQY
                     GMGRQNAAANT"
     gene            complement(16736..16963)
                     /gene="feoA"
                     /locus_tag="U6309_000353"
     CDS             complement(16736..16963)
                     /gene="feoA"
                     /locus_tag="U6309_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter FeoA"
                     /protein_id="HEN5503812.1"
                     /translation="MQISELKQGDKVRLVSFGATDMQYRRRLLSLGITCGVEFSVVRM
                     APLGCPVQIEVRGTFLTLRKDEASQLVLEHL"
     gene            complement(17051..18133)
                     /locus_tag="U6309_000354"
     CDS             complement(17051..18133)
                     /locus_tag="U6309_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEN5503813.1"
                     /translation="MSLIEQYEAAIYRGEIDSDPEQREILEHMQRLAEDLQKKNDSWF
                     PWRKKHPIKGLYIYGPVGVGKTYLVDLFYQHIDEEKKARFHFHHFMQQIDAQLRRLQG
                     QKNPLQYIAKEMAKSIRLLCFDEFLVHDVAYAMILAELLQALHHYGVVLVVSSNTRPD
                     ELYLNGVHRERFIPAIEIIKKNCEVLNLNQKRDYRLGRQPLLEAYLYPLNEQTQEAME
                     KQFALITREADRKGFITVQNRDIPYIKYGARSIWFAFDVICNFPRSQLDYLEIADRFD
                     NVFLSDVYQLTESHTAQAIMFIHFIDVMYDRGIKLIISADVPAEELYVKGEMKETFKR
                     TLSRLIEMQSVDYLGRHPRRLVKEML"
     gene            complement(18130..18792)
                     /locus_tag="U6309_000355"
     CDS             complement(18130..18792)
                     /locus_tag="U6309_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503814.1"
                     /translation="MLFTDKLNTPGEHELMLEGLVGPLEAVLTVPEDVDTRYVAFLGH
                     PHSLQGGTMNNKVVTTMARAFKELHIPSVRFNFRGVGQSGGHYDNGIGESDDMRKLVR
                     QWHSEQPQAQNIFAGFSFGSYVAYRTASYCEHALLITIAPPVHHYDYKEFEPPPHPWF
                     IIQGKEDEVVPFELVSEFASQSSQVLPVIEFVETGHFFHGKLLILKEKLMDAVRDQVC
                     LS"
     gene            19029..19817
                     /gene="panB"
                     /locus_tag="U6309_000356"
     CDS             19029..19817
                     /gene="panB"
                     /locus_tag="U6309_000356"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEN5503815.1"
                     /translation="MKIHDFKIKKQEQKKISMLTCYDYPSACIVAESNIDCVLVGDSV
                     AMAVHGHPTTIMATIEMMELHTQAVARGLGKQFLITDLPFLGHKSSQGHTVENVKRLL
                     QAGAQAVKIEGADKDTCQTISHLVNAGIPVMGHIGLTPQSIHQLGGYKIQGKNSEQAE
                     TLLQQAAALEQAGCFAVVIECVPQGLAKTITDSLVIPTIGIGAGPGTDGQVLVWHDML
                     GLQTSFNPKFVKKYFRAKDHFIEALNSYVQQVQQMHFPANEHSF"
     gene            19829..20587
                     /gene="panC"
                     /locus_tag="U6309_000357"
     CDS             19829..20587
                     /gene="panC"
                     /locus_tag="U6309_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEN5503816.1"
                     /translation="MQIFHNLNEWIHFRNTLSPDLSLGFAPTMGNLHAGHASLFLASS
                     KENHYTVSSLFVNPTQFNNPDDYKHYPRTVDADLELMTQNGVDFCILPNENEIYTDGY
                     AYQVQENRLGQLMEGKHRPGHFNGVLTIVMKLFNLVKPTRAYFGEKDYQQLLLIQGMV
                     KALFMDIEIKSCPTVREKSGLACSSRNNRLTPSQREIADEFAKIFHQNKSSAMISKEL
                     EALGITVEYIEEFQGRRFAAVKIGDIRLIDNYLL"
     gene            20584..21117
                     /locus_tag="U6309_000358"
     CDS             20584..21117
                     /locus_tag="U6309_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN5503817.1"
                     /translation="MIQTNWCVITGGPSSGKTTLINHLAELGYKTAPEIARFYINQLL
                     SHDKKTLQEVKHDILWLQRKILNIMLRRERNLPKDDLIFFDRGTPDSIGYFRFHHLDE
                     AHVVRACRYLRYKKIFFCHPLPVVPDTIRDEDDVTAKKISDYIYNAYVDLNYPVIELP
                     ATSIKERLEIILSHVDT"
     gene            complement(21131..21595)
                     /locus_tag="U6309_000359"
     CDS             complement(21131..21595)
                     /locus_tag="U6309_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503818.1"
                     /translation="MFKKILVANRGEIALRIVRACKEMGIAAVTIHSEVDRFALHVKR
                     ASHSHCLSKKPLDAYLNGRRIIECAKLTVWAFDWPSVLRRARRALEEMRMFGVKTTIP
                     YYLEMIKSEEFQKGVFNTGLVEAHPEWLKYSNKSLPQHKAAVIAAAIALNKT"
     gene            complement(21588..22304)
                     /locus_tag="U6309_000360"
     CDS             complement(21588..22304)
                     /locus_tag="U6309_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEN5503819.1"
                     /translation="MYSSNYIYQHDDLELHGFLAHEEGIKQSRPAVIVAHDWSGRGEF
                     ACLKAKQLAEMGYIGFALDMYGQGQIGSNNDEKMALMQPLYSDRSLLRERLYAALEAI
                     KSISVVDNNRIAIIGFCFGGLCALDLARSGADIRGAVSFHGLLNKPENLASGRIKAKI
                     LALHGYDDPMAKPEQVHAFCQEMTEANVDWQMHMYGQVKHAFTNPQAHDEHLGLIYDE
                     VAARRSWQAMSQFLQEIFNV"
     gene            22586..23452
                     /locus_tag="U6309_000361"
     CDS             22586..23452
                     /locus_tag="U6309_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN5503820.1"
                     /translation="MMTDSLIHIRDLLHAQFCYQMFITPIHLPIDKKYREFAKMACEF
                     IEGRRDEIIHREYPRHHVLHRFTPANKPNGKKILITHGWMSRAAYMVRLIRLLHKEGY
                     EVYAIDFPAHGEAKGIQLPWTDAIAIIKETINQFGPFYGLVGHSFGGSMILNTLNLAG
                     QLPEWQLNHKPERAILIASPTQMRTPVNKIARRFKLSGQAYLQLRQLIRQQASVDPER
                     IRLSHFISQAPDTYFLCIHGELDATINPKESINFCKYYKNARLSLLPDADHVSVLMDE
                     RVEQIALDFLNP"
     gene            complement(23587..24441)
                     /gene="rpoH"
                     /locus_tag="U6309_000362"
     CDS             complement(23587..24441)
                     /gene="rpoH"
                     /locus_tag="U6309_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEN5503821.1"
                     /translation="MSQQLQLAAMNLPVGSLDSYIHRVNQIPMLTLEEEIAYAERFHS
                     EGDIEAARQLVLAHLRYVVRVARGYLGYGLPLSDLIQEGNVGLMKAVKRFDPKMGVRL
                     VSFAVHWIKAEIHEFVLRNWRIVKVATTKAQRKLFFNLRQMKNRLGWFSNEEVDAVAK
                     DLGVSREDVLLMEQRLNVMDSSYDAPDVDDNDDAYKAPERYLFNVNDDPAVLLENEDT
                     GDQGREKLLFAMEQLDERSQDILQQRWLAEEKLTLHDLAEKYGVSAERVRQLEKNAMK
                     KIRQYMEG"
     gene            complement(24737..25666)
                     /locus_tag="U6309_000363"
     CDS             complement(24737..25666)
                     /locus_tag="U6309_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEN5503822.1"
                     /translation="MLKRSQSLLAYHLQAAANSLNLFCRKPIATMMTVIVIAIALALP
                     TLFWVFTDNLSELTNRWQRGGHISLFLKPSLPEAEQTSLLEKVRTTEGVGQANIKSAA
                     DGLSELTQQEGMQDIMRYLPENPLPAVIEVVPALVIDSPAKLDLLSRKLQAFPQVALA
                     KLDMEWINRLHAILGFSGKAANALMALLALAVVFIIGNTLRLDIHNRQEEIKILKLIG
                     ATDPYIIRPFLYSGVWYGAAGALLAIFLVNIFILTLGVAVNQLANVYQMHYPLACLSL
                     RQILLLVLFAIILGWLGALLSVKRQLASIEPYK"
     gene            complement(25660..26310)
                     /gene="ftsE"
                     /locus_tag="U6309_000364"
     CDS             complement(25660..26310)
                     /gene="ftsE"
                     /locus_tag="U6309_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEN5503823.1"
                     /translation="MITFDQVSKRYPGGFEALSQVNFSLQKGEMVFLTGHSGAGKSTL
                     LKLIALLEWPTSGQLTVNGLKLNHLKKRDVAAHRSQLGITFQSPQLLNDRTVFDNVAL
                     PLQIQGVAYPMIAKRVHAALDMVGLLSKEKMLPVHLSVGEQQRVGIARAVVHKPALLL
                     ADEPTGNLDPKLSSEIMKIFEQFNQVGVSILIATHDLALIAGMKHRIVMLKGGRLC"
     gene            complement(26297..27364)
                     /gene="ftsY"
                     /locus_tag="U6309_000365"
     CDS             complement(26297..27364)
                     /gene="ftsY"
                     /locus_tag="U6309_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEN5503824.1"
                     /translation="MIKWFKRNQNSEPNLDTEEQQDHAESSMESRLEEPQELTAKEPS
                     PKESIFARFRKGLSKTRHQLGEGISRLLLGKKEISQELLEELETLLISADLGLDTTQD
                     VLKQLNEGLARKQLSNGEAVYQTLKTNLQTILSQEEKPLSIETEDGSPFVILMVGVNG
                     AGKTTTIGKLAKQFQKQGKKVMLAAGDTFRAAAVEQLHVWGERNGIPVIAQHTGADSA
                     SVIFDALQAAKARKIDVLIADTAGRLHTQSNLMEELKKVKRVMQKLDPKAPHETMLVL
                     DASIGQNALAQAKQFHEAVQLTGITMTKLDGTAKGGILFAIANDLGIPFRYLGVGEGI
                     EDLRPFDAAQFVSAIFDDDHI"
     gene            27427..28752
                     /locus_tag="U6309_000366"
     CDS             27427..28752
                     /locus_tag="U6309_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN5503825.1"
                     /translation="MHKLLISLMMLLSFQAFSQVQEYTLNNGLKVLVKEDHRAPVVVS
                     MVWYNVGSADEPVGITGVSHAIEHMMFKGTSKYPVGVFSKTIAALGGQENAFTNNDYT
                     AYYEKLDADHLATSFELEADRMNNLLLNSEEFAKEIKVIQEERRLRTDNNPQALAFER
                     FLATAHLTAPYNHPVIGWMNDLKQMKVEDLKKWYESYYAPNNATLVVVGDVNPEKVHA
                     LAERYFGSIAKRPIASRKPQQEPSALGKKMVYINAPAKLPLLLIGYTVPSVKTAKNNW
                     EPYALEIIAGILDAGESARFAKHLVRGNQVATGAEAYYNLYSRYQSQFIVYGAPSQDH
                     QIKDLEKALITELEALKKAPVSNQELQRVKNQIIAQKTFEKDSIFGQAMELGLLETIG
                     LGWKNTETYTKAINEITPEQIQQVAQRYFQENNMTVAELKPIRQEEVRP"
     gene            28749..30053
                     /locus_tag="U6309_000367"
     CDS             28749..30053
                     /locus_tag="U6309_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN5503826.1"
                     /translation="MSVLRAFIISFSVIFSHNLFANSFKTEKWQTKNGVRVVFYQAME
                     VPMLDISLAFAAGSAYDSKYFGLSALTTNLINQGNSGKDATTIAEALADTGAQFNAET
                     SRDMVVLSLRTLTSKEALQQSTKTFSQIISHPDFPKEAFAREKDQLLMAVEQTEESPD
                     DVAIQNFFKTLYQEHPYAHPVHGTVESLNAIKENQVIDFYKKYFVAKNGILVMVGAID
                     SSQAHQLAEQLTQDLPAGEPAPTIPKASQLADAEKINVPFPSSQTVVRLGQIGIDHHN
                     QNYFPLMVGNYILGGGTLVSRLGTEVREKRGLTYGIDSQFVPMLGEGPFIISLSTKNS
                     EARNALHITQDTLIKFIKNGPNKEELTSAKQYLTGSFPLSLGSNTNIANLLLRMAFYH
                     LPDNYLDTYVAKINAVTDAEIRQAFQQQVNPEKLLLVTVGQS"
     gene            30050..30595
                     /gene="rsmD"
                     /locus_tag="U6309_000368"
     CDS             30050..30595
                     /gene="rsmD"
                     /locus_tag="U6309_000368"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEN5503827.1"
                     /translation="MKQVIRIIGGLYRGKKIHFPDVDGLRPTPDRVRETLFNWLMNDI
                     RNARCLDAFAGSGALGFEAYSRGASHVVFIEQSPKAHASLQNTIRQFDTPNLQLIKTD
                     TLNYLKQSTEQFDLIFLDPPYALNYVPQCLDHIITNNLLVKGGLVYIESSSPIEVKSE
                     LWKQLKLKQAGQVIYGLFEKL"
     gene            30670..31161
                     /gene="dotD"
                     /locus_tag="U6309_000369"
     CDS             30670..31161
                     /gene="dotD"
                     /locus_tag="U6309_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system lipoprotein DotD"
                     /protein_id="HEN5503828.1"
                     /translation="MNNNKIVIMFIFSALLAGCAGTMKFKKPPINNPSDDATIKLAEA
                     AVSVSDSMLEMAKVEKVITPPSKDNTLTIPNAYNLQARASVDWSGPIEELTARIAKAA
                     HFRFRVLGKSPSVPVLISISTKDESLAEILRDIDYQAGIKASIHVYPNSQVVELRYAK
                     IYS"
     gene            31142..32053
                     /gene="dotC"
                     /locus_tag="U6309_000370"
     CDS             31142..32053
                     /gene="dotC"
                     /locus_tag="U6309_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein DotC"
                     /protein_id="HEN5503829.1"
                     /translation="MRKFILSLSILLSALLVACSSRNHYGDTGSLAGLQAMADSKYTR
                     AQKKQKMGKIREMALKETALSVGAQAGLAWRAKIIDEQLNKQARNLDAIYDFNSLVLE
                     HNILPPVLLEGRNTLNLADAQSIRVSDRTYKVAKQAHFITTPPTWRQYLWMDYVKPEA
                     PNVTLLPKTKAEKEIWCIYTERGWKNGIDQANTILEENIARIKEDFGGMILYRKLLAM
                     NMVSPPYVSHTDLGVTGDGSEIHIDDRVLRITALPELNVNSAEWRAAVAKDENALERF
                     KNMEKLANQAKIVITNKSWQPIIAPVS"
     gene            32053..33186
                     /gene="dotB"
                     /locus_tag="U6309_000371"
     CDS             32053..33186
                     /gene="dotB"
                     /locus_tag="U6309_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system ATPase DotB"
                     /protein_id="HEN5503830.1"
                     /translation="MSMDNINLMPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPI
                     FAEVYGRLLKITNRRLSNTELGDLINSIYGPNATTQLLSGKDIDTHYEFRPNRGVRYR
                     YRVNATACLVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEGIVFITGATGS
                     GKSTLLASIIRELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFAD
                     GVRNALRRKPRLIMVGECRDAETISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFS
                     GEERLGRTIDILETIRLCIWQKLVPTVDERRVALREYLVFDEEVRDILLEGDPNEVTS
                     ATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLIIAGAKEYQQ"
     gene            33272..34996
                     /locus_tag="U6309_000372"
     CDS             33272..34996
                     /locus_tag="U6309_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEN5503831.1"
                     /translation="MFHRKSLYHYFNLFILLILGSSLNWASVNQHGTDSPTLNIKILG
                     INDFHGQISSGRAVKNEPVGGAAVLAAYLKEAQLGMEDRSIITIMGDLVGASPPSSGL
                     LNDEPSILFINGLGNNQCKPESRMDPLCNIVATVGNHEFDKGQKAMFDLIYGTNNPPT
                     DSWISLPSYPGASYPYVSANIVDAKSEQPLFPPYIIKRIHNTPIAFIGAVLKDAADSM
                     FPANAEGIKFLDEADSINHYIPEIKAKGVNVIIVLIHEGGNQVPYEGDTQAASAVEGH
                     IKEIVYSLGDEIDVVMGGHTHQFLNAFLPNRNGHEILVTQANSYSAAFAEVTLQVDTR
                     NHTVKNKSAKIITTYANRWPGTNPDTQALEIVKLAEDKIGPIINSHVGTAEYDLLRKQ
                     NEDGESSLGNLIADAYRTVMNTDLGITNPNGIRDDLKAGEVNWGNIYSILPFSNRIVI
                     ITLTGNDIYDLLEQQWMGSYINMLQISGFAYTYDSHQPLGHKIIEIRHQNKPLLREKM
                     YTIATSDFLASGNGVFSVMKRGKIIHIGQSDHDTVIAYIKSLPQPFHASIEARIKESE
                     TGLKTGVG"
     gene            complement(35038..35835)
                     /locus_tag="U6309_000373"
     CDS             complement(35038..35835)
                     /locus_tag="U6309_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5503832.1"
                     /translation="MTSTYILNTNEKARERLSLQHQLYAKSSLDLLSEVGPSAAMTAL
                     EIGCGSGDMTLELAKLIGREGNLLTIDLSQEQLDHTQQRTRTYSNIRFKVWDVNHLSD
                     LGEKFDLIYCRMVLHHVADARSVILQMKNCLNSGGFIVCEEPSLFDSTFCSPPSPSYD
                     QFTQWARSCFSKNNRDFQIAHRLEQEFSYCGFEIEHCSLFQPLLRTNKEKLIYSMALD
                     DLTPQLLELGIAKREEIKQLHNELEDLAKTNSTMCWIRMHRIIAKLN"
     gene            complement(35865..36809)
                     /locus_tag="U6309_000374"
     CDS             complement(35865..36809)
                     /locus_tag="U6309_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN5503833.1"
                     /translation="MKPSVYLTNQFLEPIIPMLIPDWNIIHGWKMASGVDQSRVVALA
                     TTVWDQIDHSFLMQFPNLKIISHLGIGTDNIDINFLKQNHIILHSQPNAGVHDTAELA
                     IALLLALSRRVILNDRYTRNNEWVEKKPRFLGNHLLGKQLGLVGFGQIGEKIAQFAEP
                     FGLKIAYTARSQKSSPYLYCPTVASLASISDFLIVCCSGGVETQHLINKQVLDNLGAE
                     GYLINVARGSIVDQNALIDALQRHAIAGAALDVYQYEPEVPFALRQLDNVVLSPHMGS
                     STKENLNQMFQLQAKHLSQYLSEPLWQRESHVTLFEEQ"
     gene            37085..38107
                     /locus_tag="U6309_000375"
     CDS             37085..38107
                     /locus_tag="U6309_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl peptide synthase"
                     /protein_id="HEN5503834.1"
                     /translation="MPVIPVEEFDGFILKLGKNNYHFCKNETPFNNSCSANIAIDKYC
                     TSKLLEKAGIPVPKAISLNCSEFQRNVHKDKIAQLNFPLVIKPIDGSLGIGVLCNIKT
                     WEELESHLTKYFSSYQCLIIEEFHGKLNSYRVLVFNNRILGIVQRYPATVTGDRVHPI
                     HELIKQENLNRKRINEALGEITLDEETRIKLQELGITENYIPSFGEQIVLCYTSNATR
                     GGSYVTINKKMCKQNRKLLLQAAKILDLQLVGIDVECSDIINVPIEQSKGVILEVNHK
                     PSIRIHEFPMKGTPQKVTKKIMRNFIIRHPLSYLYSIYNNQPTAFYVRSSILIIIMGL
                     ILLAVW"
     gene            38381..39268
                     /locus_tag="U6309_000376"
     CDS             38381..39268
                     /locus_tag="U6309_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEN5503835.1"
                     /translation="MNPVSILDASLRDGGHRTNFHFKDDDLKQILLPLDNSGIEYIEI
                     GYRNGSLHPIENIGKAGMCPQDYLLYCQSLIKKAKIAVMVHPHNVNEQDLLELKNCGV
                     KLIRICIAKGDLVKAVPLIKLAKNLNFETSVNIIHISYYTESELDSLIEEVSKHEPDM
                     IYFADSNGSLFPDKINSLYKKYTSQYKVSFGFHAHDNLGLAQANALAAVNAGVHFIDA
                     SLAGMGKGTGNLKTEFFIAYLHANNIKKYNLEDVLTAANYVRDALKIGQEPIEMSEFI
                     RGISDLSTADLKSYKPGKL"
     gene            39407..40114
                     /locus_tag="U6309_000377"
     CDS             39407..40114
                     /locus_tag="U6309_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503836.1"
                     /translation="MAKKRLLIIGPVSTQTSSLKHLSQNLSFLNPYYSIECLDPLVTL
                     QECPDEEYYGIWQNQLKSLIKIYDAFVGFSFGGVILQQCFSILSGEKKQIILFSTPTF
                     ADSILREKLETVISLCSQNKLEEGLSSLYQAVYAPHSIPDASLQYLNRELAIKRLIFG
                     LTKVLKTNCVSILNHCNVNHLHLIGEKSQLVNINNVIKPSCGQLIVVPEASMRVLESN
                     PTFCKEIIFKALNHEIH"
     gene            40101..41948
                     /locus_tag="U6309_000378"
     CDS             40101..41948
                     /locus_tag="U6309_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEN5503837.1"
                     /translation="MKSTDPIKIAVLPGDGIGIEVTEATLPVFEVLDVPATLSYGDIG
                     WEFWKKEGTAIPSRTWQLIAASDTVLLGAITSKPQREAKQELSKALRKSNPYYVSPVI
                     QLRQGLDLFANVRPCFSLDDQSKPFNFCIIRENSEGLYCGFDYFPLPKAIHNLLAESQ
                     HWQTIPADEASCALRLQSKPGLTRLFDFAFNHAMQTGMPRVTLADKPNVLRESGEFAR
                     KIFESTAQKYPKIQADILNVDAVALWLIKSPEKFGVIVAENMFGDILSDVGAGVMGGL
                     GLAPSANIGDKGSYFEPVHGSGPRMKKNCANPSAMFLTISMLLNHFGYPDRAKKIVNA
                     VMQVIKEKRFITYDLGGHSTTTDMANAVIERCARLEASCLSTDFSSVPKENLIESDTM
                     SNLLQQLMHCNSAEISDALDACGIEGGLLNIKPLSQGMKMVGPAYTIQYLPREKKETA
                     FHNAANYIDKVPKHSVIVIDNNGQTDCTVWGDLLTHTALRNNIMGTVVHGAVRDVESI
                     RSTNYPVFCTGIYMCSGKNRVYKANEQCPLFINGVTINPGDIVFADDNGVLVIPNELL
                     HEVVNKAIAIRLTEERIKAAVASGSTLEQAREDYYYEQPWLGINKKRKQ"
     gene            41948..42808
                     /locus_tag="U6309_000379"
     CDS             41948..42808
                     /locus_tag="U6309_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503838.1"
                     /translation="MFLPEKLKFIDIHYHAAPDLYIRRRNAIEAGRLYHSLRGAAVLK
                     SHIGSTGIQATIAQEMGLPVFPSVVLNHINGGINYRVVIRALSEYQPLFSGKMIVDFP
                     TITGRKLQSKLSRDLTHNTLKQYTGQKETLFGSKQQLRKEVIDILKMARDYPIVLTSG
                     LASREEVYRLIDACNQYNVPSLLLSQPSNPLTGLKFTELNELIKNEWLWVEQTALTFI
                     LEHQDKQDFSQVLTHLPRVIYSSDLGQTSQMDIPEWLHFSKRIFNELSLSEKRMEELF
                     LSNAITLLGL"
BASE COUNT        13584 a         8959 c         8123 g        12273 t
ORIGIN      
        1 ttgtgcttat tctcttcttt agtaattcga gccttgcgaa aacaatcaat tatccatgga
       61 cagtctccgc aagcttgggg tatgtcaatt atcaatacgt acacgaaggc gaccatatta
      121 ccgcaacagg tcggtttgca ataggaagag atttgttgat gaaaggcccc tttttatttg
      181 gacttgaatt aggtttacaa agtgggaatc aaatgcgact gcaaatgacg gaagaagagg
      241 tcgatgccct cggaggactt ttggtacaaa ccaccttgaa acctatggtt gatgcattag
      301 ctacaatccg tattacccct gaatttaccg cccctttgtt tctacaactg aaagcaggaa
      361 ctgcctacag gcaatggcaa atggatagag acacgatcaa tgacatctct caacttgcag
      421 gcgaaataat ggctggatgt ggttatacaa ttacaccaaa tgccagtatt accctatcgt
      481 atcaaggtct atttggagac aaaccaaatt tcactattga tctggaaagt ggcattggcc
      541 atgtacaaaa gattcctgtt caacacgcca ttttgttagg tattgtttta atgatttaac
      601 tcatttttct aatagatctc ttgcataacc tgcttatttt gttttagggc aaggtacaaa
      661 cgttttgagg agtaggattt acaagcagta aatgagcacc gcagaaacgc ttacaacgca
      721 gcggtaaaat aaaagagaca agtgagcata cagtgggata taaagaatga tacatcgaaa
      781 taaagttctt aatagcattt tattaatctt attcacatca cagatagcat ttgccaaaag
      841 caatccggcc tcgcaagcct atgtggacgg taaggtatct gaattaaaaa atgaattaac
      901 caacaaaatc aacagcatcc cctctggacc tcaaggacct aaaggagata aaggcgaggc
      961 tggccctaaa ggagaccgag gggaggcagg gccgcaggga ttaccaggtc caaaaggaga
     1021 ccaaggggag gcaggaccgc agggattacc cggaccgaaa ggagaccaag gggaggcagg
     1081 accgcaggga ttacctggtc caaaaggaga ccaaggagaa gcaggacctc agggattacc
     1141 aggcccgaaa ggagacaaag gagaaacagg acctcaagga ttaccaggcc cgaaaggaga
     1201 ccgaggagag gcaggatcgc agggattacc cggaccgaaa ggagaccaag gggaagcagg
     1261 gccgcaggga ttacccggac cgaaaggaga ccaaggggaa gcaggacctc agggattacc
     1321 cggacctaaa ggagaccgag gggaggcagg acctcaggga ttaccaggcc caaaaggaga
     1381 cagaggggag gcaggaccgc agggattacc cgggccgaaa ggagaccaag gggaggcagg
     1441 gccgcaggga ttaccaggac ctaaaggaga ccgaggggaa gcaggacctc agggattacc
     1501 aggcccaaaa ggagacagag gggaggcagg accgcaggga gtacccgggc cgaaaggaga
     1561 ccgaggggag gcaggacccc agggattacc aggcccaaaa ggagacaaag gagaaacagg
     1621 agcagtaggt ccacaaggta tgccaggacc taaaggcgaa gcaggagatg acggccaagg
     1681 tgtgcctgca ggtggtgaaa cgggtcaagt ccttgccaaa tcaaatgacc tcgatttcaa
     1741 taccatgtgg gttgacccgg caaattccgg tattaggcgg caattaggcg ataaagctct
     1801 tggcggtaca gtaatttatg ttaatgcgct agggacccat gggcttgtag tagcaaattc
     1861 agatcaagtt aattcaagca catggtggga tgctcaagat tccataacca atcctgccca
     1921 ctttgacaat gaaggtaaat tatattctga ctggaggctt cctagccgct ttgaattgaa
     1981 tttaatttat atgatgcgtg atgagctggg aaattttttg gcaggcaatt actggagctc
     2041 gattgaaaaa tcatcggcaa acagctgggt atttaattct aaaacagggg aaattaaaga
     2101 cattgccaaa aataaaacag ctgctgtccg tgctgtaaga gccttttaag tatggcaaaa
     2161 cttcaatttt gctcaaggat gaaaatgttc atgaattcgc tttgccaact gctcgctgat
     2221 tccctggact ttacaaattt cttccagtgg cgctttagcc aactctcgca acccgccaaa
     2281 acgctgcagc aacgcttgac gtcgtttcgc accaacaccc tctatacttt caagagtgga
     2341 ttcaacacgt gtcttctgtc ttttcttcct atgggcagta atagcaaaac gatgcgcttc
     2401 atcacgaata tgttgtaaaa gatgaagcgc tttcgagtcc tctggcaaaa caaattctct
     2461 gttttcatta accagtatca atttctccca tcccgccttt cgtgaaggcc ccttggatac
     2521 acccaataga gtaatatctt ccacacccag agaagttaaa actcgtttta caatagaaac
     2581 ctgaccctta cctccatcaa taatcaaaac atctggcaat aattgagcat caatcagtct
     2641 tttaaatcgg cgagtaactg cctgttccat tgctgcgtaa tcatcccctg gagtaatacc
     2701 ctcaatgtta aatcgcctgt attcactggg gcgagggcct tctgtatcaa atacaacaca
     2761 agatgcaaca gttgcttcac cttgagtatg gctaatatca aaacactcca ttcgctctat
     2821 atttttatca agcgcaagca attgctttaa tgcctgatat cttgatctta tcgtagaatg
     2881 tttgctgaca tattctgcta ctgaaattcg taaattgttc actgcaaaat ccatccatct
     2941 cgatttgact cctcttggat tgacttgaat tttacaggat ttgcctcgtc gctgggacaa
     3001 aatatattct aatgaacgac tctctgttat cgattgatta gtaattatca aatccggaat
     3061 tctttctgat gtatccaaat aataaaaacc aacaaaggct tcaaaagtct gctgccaaag
     3121 tgagttggca tccaattctt catctaatac tgcataaggc actgtaggga aaaagctttg
     3181 actattcagt acttgtcctt cacgaatagt cacacactga atacatgcga aaccagggcg
     3241 tacttctatt gcgatcacat cagcatcacc acgtaactgg acaactcctt gttgttcttg
     3301 aattaagcgc aaatttttta tctgatcacg caaaacagca gcttcttcga aatttaattg
     3361 attcaccgct tgtttcattc gttcggcaag ttcatcaaga attatttgac acttcccctg
     3421 taaaaatctt atggcatcct ctacagacaa tttgtagttt tcaggagaaa tataatgaac
     3481 gcaaggcgcg gtacaccgtt ttatttgata ttgtaaacaa gggcgagaac gggctttaaa
     3541 ataactgtct ctacaattgc gaattttaaa tattttttga atcgtgacta ttgtttctct
     3601 tacagcagct accccaggat agggaccaaa aaaattaccg gagggcggct tctttttact
     3661 tcggtataac tcaattcttg gaaaatctgg atgatttgat agatgaatat aaggatagga
     3721 tttgtcatca cgcaacaaaa cattatattt tggccttaac gctttaatta aattactctc
     3781 taataataag gcctctgtct ctgatcttgt cacagaaatt tctatggatt tgatttggct
     3841 taccagtgct cgtgtcttaa ctcctgtatt ttgtttacta aaatagctgt tgactctttt
     3901 tttaagattg gctgctttcc ccacatatag tacggtgcct tcctcgtcta acatgcggta
     3961 tatacccggt tcacttggga gtttcgttaa aaataaagcg agttcagcgg aaagctgtaa
     4021 gtcattcatg aaattaacat ctaatcatct tgtgacaaat cattggggtg ttcaataatg
     4081 cggtgcttca tagccaaata agtaagctcc acatcatttt taatccccaa tttttcaaac
     4141 atcctatatc gataaccatt gatggtttta ctacttaaaa ataatctatc agcaatgtct
     4201 tgtacattca tgccgctcgt aatcatcaac atgacttgca tttctctttc agacaataaa
     4261 tcaaaggggg aatcttgagg ctcctgaaga ctattaattg ccattttttg tgcaatctcg
     4321 gcactaagat atttctcacc tttagccact ttacgaattg cagcagccat ttcttcagcc
     4381 cctgattctt tagtaaggta tcccatagca cctaattgca atactcgagt agggagagga
     4441 tctgagcaaa tagctgtaac ggctatgact ttaatatttg ggtttgtttt ttttaagcga
     4501 cgcgtcactt cccacccatc tatcccaggc attttcatgt ctaataatac gacatcaggg
     4561 gagtgggttt tcacataagc taaagcctgc tccccacttt cagcatctgc aaccacatca
     4621 acatccgaca tatcttcaag caatcgtcga atgcccattc taaccaatgc atggtcatca
     4681 acaattaata ctttaatcaa ataatgctcc ttgaccaatc agtcaatgaa caaactgtgt
     4741 tggtcgatgt taacaagact cgataatcat tacaatacat agtcactcta atccacaagt
     4801 ccagctgtct tacaaaataa caaactaaca agcccttata tgaatatttg gattatccgg
     4861 atccacatca aaatacggtg gaaattcacc taactctcgc gcacgatcca tgaatttacc
     4921 catatcagaa agcacgaagt cgtataaatt ttgatagctg tcaataacga aataaacagg
     4981 ttgtaaccgg tcaatccgat aaggcattcg aaaagccact actggatcaa ataaaattcg
     5041 tacaggaata tcactttcca cacaatatac cgtttcacta atagaagata aaattccccc
     5101 gccgtatgct ctaagccctt taggcgtttt aatcaatcca aactctacag taaaccaaaa
     5161 cattctttgc aataaaggcc aatcctgttc aggaaaagtt aataccttac atgcgtaatc
     5221 atggacaaat tcagcataga ctctatcggt taacatagga caatgaccaa aaagctcatg
     5281 aaaaatatca ggctcttgaa cataatccaa ttcttcttca cttcgaataa aagtcgccgc
     5341 aggaaaatat ttttctgcta ataattcaaa aaattccctg gctgaaatta aagcagcaac
     5401 tggtgctact tgccatcccg ttttggcctt taatcgctca cttacttctg gaagttgtgg
     5461 aatagtcgag gagttaagtc ctaaagtctg taatccagac agaaattcat cacaagctct
     5521 tcctggcaat aacttgagtt gtctctcaaa taaaatattc caaattctat tttcttgtgc
     5581 cgaataatcg actaaaccct gagcatcagg aacatgtgcg acataccggc tactaaactc
     5641 cataatctct ccttcactca tctcattcaa aaatcaataa ccagggataa taatatttat
     5701 ttgtatttgg ggtcagccta ttaaagtcaa cgagaatttt tacctaaaag ttgctttatt
     5761 ttgagtatac atcctagcca ataccctgtt ttagattggc attattatga tgatcactaa
     5821 actctctaac ttgttttagt aattacctaa cagtaaacta aatgtcaaca tgattactat
     5881 tatctttttg agataccctc taactaacaa tggaaagcaa tgagtaactt tccgatatgg
     5941 caaaaattac caactggtgt atgtggggta actggggcat cataagacga taacttaatc
     6001 tgttaacact tatatattaa ttattactta ttttacagat tctgctatag aatttattac
     6061 agaaattacc cattggaata cacaaaatga caatagctat cttgtctacc ggagatgaaa
     6121 taatacatgg cgatactcta aataccaatg cgcattacat tgctcatgcc ctttgctctg
     6181 aaggcttacc tcttggatta caagtcgcct gtagcgataa ggaaaatgag attatagata
     6241 gcctaaactt cctgacggaa caccatgata ttctaatttt aattggtggg cttggcccaa
     6301 cgaaggatga tattactcgc tttgctttgg ctaaatttac caaacaacct cttattcagc
     6361 atgctaacgc tctggctcat atagaaaaaa aagcaaccat agccaaaata tcactgaatc
     6421 aaggcaattt gcagcaatgc ctctttccag ccaatgcacg actttttcct aatccttatg
     6481 gcacagccat gggatgttct tattcatgga aaggtaaagt ttttattcta ttgcctgggc
     6541 ctcctcgtga atgccttcca atgtttaatc aggatgtact gccgttatta ttgcaaacat
     6601 ccaggcatag caataaacaa attctgcgct ggcgaatttt tggcttggct gaaagtgaaa
     6661 tagctcaaat ccttgaagaa gctctgcaag gtttagactg tcagacggga taccgattgg
     6721 aaacgcctta tctggagttt aaggttcgct gcaaaagaga attgattgaa aaaataacat
     6781 caataataga ccccatttta gccccgcaca ttatatcatc acttgaaacc aaagcatctg
     6841 atcaattgag aagcctgatc ttaaagaata atgaaccaat aactattatt gatgatgtta
     6901 ctggaggatt attgcaatcc ctattggtaa gaccagaaaa ctaccattta ttaaattttc
     6961 ataatcttaa caggaccaaa ttatattttc attgcagtgg attagaagat tattggtcac
     7021 aacaagcacc acaaggcact accaaattaa tcattcacta cagcaatcaa atgcaagagg
     7081 gtcgtgaaac acatcagatt ccttatcgaa gtgctatggt tgtgcattat gccgctgaat
     7141 ggctaagctt cagactcttt catctcatca atcaattgca ttaaagcgta gcaaagttct
     7201 tgtgttccct tactctctat agcggaaata gaaaatactt tgtctttcca cttcaagcct
     7261 tttattatgg attgaatttt ttcttctctt tccttttcat caggtaacat atcgatttta
     7321 tttaaaacca gccatctggg tttgttgagc agatcgggat tatattgagt caactcatta
     7381 agaattgctt tcgcatcagc gacaggatca ctcccatcca aaggcgcaat atcaataaca
     7441 tgcagcaaga cacaggtacg tgaaagatgc tttaaaaacc gatgccccaa tcctgctcct
     7501 tgagccgcac cttctataag acctgggata tccgccatga caaaactctt atagggtgaa
     7561 acacggacca cccctaaacc tggatgtaag gttgtaaatg ggtagtcagc cactttggct
     7621 tttgaactgg agacggctct tattaaagta gactttcctg cattgggaag ccctaacagt
     7681 cctacgtctg ccaaaactcg aagctccaaa cgcaaatttc tggattcacc agggcttccg
     7741 ggtgttgttt gtctaggtga tcgattcaca ctgctcttat agcgagtgtt acccaaacca
     7801 tgaaacccac cctgagcaat cagcacggga acacctggtt gactgatatc agctaacaat
     7861 tcacctgtat cggcatcata aaccatagta ccgactggca ctttgatgat taaatcctct
     7921 cctttcttcc cagtacaatt tccacccatc cctgattgtc cgttctctgc tttgtattga
     7981 cgagtataac gaaaatcaat caaggtattt aaatcgctgc tcgcctcaaa ataaatactg
     8041 cctccatcac ccccatcacc cccgtcaggg ccaccacgag gaataaattt ttctctcctg
     8101 aagcttaagc aaccattccc tccttttccg gcttcaactt taataagtgc ttcatcaacg
     8161 aatttcatgc ctaaccttag actaaaatag gacttacgca aaattccaag ctcgaagcaa
     8221 aaaatatttt caatgaatgc gtttagataa gctaagtgac tgaattgaaa atatttttta
     8281 gtaaagagat tgggattttg cctaagtctg taaaaaaaaa ccccgtttcc ggggttaaac
     8341 aaaacgaatt atgatgccat tgggtaatag cattgtttca gcttaactcg cagcttcttc
     8401 tcgttgctcc ggtaatatag tgacatactt acgatttttt tcacccttgg tagtaaactg
     8461 tactaacccc tctaccaacg catacaaagt atgatctcga ccacaaccaa caccaggtcc
     8521 aggatgaaaa cgtgtgccac gctgccttac aatgatttca ccggcattta caaattgacc
     8581 accaaaacgt ttcacaccga ggtactttgg attcgagtcg cggccattac gagtactacc
     8641 acctgctttc ttatgagcca ttgctatctc ctcttattta ccaatcgcag tgattttaac
     8701 ttgcgaataa tattgtctgt gccccatttg tttcatatgg tgcttacgtc gacgaaattt
     8761 aataatttta acttttttgt gacgaccatg atcaagcact tcagccttaa cagttgcttt
     8821 agcaacaaaa ggtgtgccac aagtgacctt atcaccatcc actagcatta atacttccga
     8881 aaatttaatt tcattgccaa cattttcagg cagcatttca atttttaaaa cgtcaccttc
     8941 tttcacggta tattgcttac cgccagtttt aattaccgca tacataacta ttctccaatt
     9001 cgcctgattg gctatcacaa ttccaacaaa gtgccatatt ctatgaaatt atgactatta
     9061 cttcaagttc attttaaaat attgtatact tcaccaccct ttaccgattg acttaatgtt
     9121 tttgaggctt tcgagtaaat tttaatctgg tcgcggatta attaggagct atagtcatat
     9181 ttaagatagt gcctagaggg aacccttaaa tttttggagt ttttataatg tcaactattc
     9241 aattagaagc acaatcaaga acggatatgg ggaaaggtgc gagccgccgc ctacgtcgtc
     9301 ttgaaaataa agttcctgct gtcatatatg gcggaagcaa aaaacctatg gcaatccatt
     9361 ttagccacaa taaagtgatt aaggcattgg aaacagaaag catttattcc agcgtttttg
     9421 acatcacagt agatggcaaa gtagagcacg ttattttgaa agccttgcaa cgccatccct
     9481 acaaaccaat cgtgttacat atggatttac aacgcgtttc cagtaaagac attctggtta
     9541 aattagtacc tgttcacttc ataaatgaag aacaatctcc aggaattaaa gctggcggta
     9601 ttgttcaaca taccatgact caagttgaaa tacgttgcca ggcaaaagat ctgcctgaat
     9661 ttattgaagt agatatgtct aaggtaggta tggatgatgt agttcactta tcggatttaa
     9721 aacttccaaa aggcgttcag ctgacagtgg atgtagccga tggtagtcat gatgctcctg
     9781 tggtgagtat acatgccgct aaagtaagtt caactgaact ggaagaaaca cctgaagttc
     9841 cagcatcggc tgtaccgact acagatcaag gggaaagtgc tgaataagca cttccctgtt
     9901 atatatttat gcaaatattc agagcttaat ccagagtatt tgcataaaat ctagtgctag
     9961 aatagtcaac acaaatccgc ttaattcaaa ttttatagaa aatctatggc cattaaacta
    10021 attgtcggtt tacgcaatcc cggatcggct tatgaacaaa ccagacacaa cgcaggtgca
    10081 tggttagtga ccgctttggc ccaaagacat aattcgcatt ttaagattga caagaaaatg
    10141 caagcggaat taactgaaat tgatattaac aaccacccat gtcgactagt gcttcctttg
    10201 acttttatga atcatagtgg tcaaacaaca agaataatca gtcaattcta taaaattgaa
    10261 cccggtgaaa tattaattgt tcatgacgag ttggatttac ctgttggacg aatcaaatta
    10321 aaaacaggtg gtggccatgg tgggcataat ggtttgagag acataactgc tcaattggga
    10381 acaggtgagt ttcatcgatt acgtataggg ataggtcatc ctggacataa agatctagtc
    10441 catcaatacg tactcagcag gccttctatg cacgacaggc aacaaattta tgatgcaata
    10501 gacagaggta tagcgataat acctatagta ttgtcagggg atatggcccg agcaatgaat
    10561 caagtaaacg cttaacataa tgaggtgtga ctcatgggat ttaaatgtgg aatagtagga
    10621 ttgcccaatg tagggaaatc gactcttttt aatgcattga ctaaggctgg gatagaggcg
    10681 gccaattacc cattttgtac cattgagccc aatgtaggta tcgtcaccgt tcctgattca
    10741 agattggata atttaagcga catagtcaag cctcagcagg tgattcatgc caccatgcaa
    10801 tttgttgaca ttgcgggatt ggtaaaaggc gcttccagtg gagaaggatt aggaaatcaa
    10861 tttttggcca atattagaga aacagacgct attgctcacg tcgttcgctg ctttgacaac
    10921 tcggacgttg ttcatgtgga ggggcgagtt gaccccttaa gtgacataga agtgattaat
    10981 acagaactgg cattagcgga tatggagaca ttggaaaaat cgctattaaa agtagggaaa
    11041 aacagtaaaa gtggaaataa agaagccatt tttgaattaa aaactctgga aaaaattaaa
    11101 gcacatcttg atgccggata tcctgtaaga acacttgaac ttacccctga ggaagaacaa
    11161 gttagcaaac gcctttttct tttaacggca aaaccagtac tttacatagc caatgtcgat
    11221 gataacggct atgaaaataa cccgctcctg gctaaagttc aggctctggc ttcccaggaa
    11281 aatgccagca tagtagccct ttgtgcagca acagaagccg agctggttga gctggatgaa
    11341 gaagacagac aagagtttat ggttgatttg gggcttagtg aacctggatt aaataaagtg
    11401 atccgtgccg ggtatgaatt gctgggattg caaacctatt tcaccgcagg agtgaaagaa
    11461 gtaagagcct ggacaatacg caaaggcgcc acagcaccgc aagctgccgg ggtcattcat
    11521 accgactttg agaaaggatt catccgcgcg gaagtgattt cttacgatga ttttattcgc
    11581 tttggcggag aacaaggtgc caaagaagct ggaaaattgc ggcttgaagg caaggaatat
    11641 attgtctgtg acggagacgt catgcatttt cgctttaatg tttaatcatt agtattctgc
    11701 aactggtgat ttttcaagaa ttgaaccaaa gagcctcaag cctctatcgt ttcatcattg
    11761 cgagtgacca aagcatcgaa ccaggcattg aagcaagcgc aagtaagcta ggcatgtgtt
    11821 ttactactct tataccacac tgaccgttgc ttagcttgcg atagctcaaa ccgacgccga
    11881 gtcaacagaa gataaacgat ttggtagaaa atgaatagca aaccaactcc ctgtgcgtaa
    11941 atcaacctca atgaggacag tgtggccgtt ttttaatcta taattgaatt aataaaagaa
    12001 attagcccca ttgattaccg gccaatgcga ttgaggtagg tgaatgtcat tactaaagaa
    12061 aacaagagag caggattact caatcctggt taccactcaa gaccagcaaa gaaagcgcca
    12121 tttacttaat gttatttcca tagaactttt aattttgggt cttttgctga taataacaaa
    12181 cctctgcctg ctaagagcct ggcttataat cagtatgtta atcgtgggca tttccatctt
    12241 gctcggaaat ttaatcttaa tcaaaaagag cttcaatctg gccttgagtg ggcacatttt
    12301 gaatattctt gtgttatcta tcatcattgg aggcaatctc atcgttggaa aaactaccat
    12361 gtcttacctg ggatggttct atgtttcccc aatcgttgct tttgctacac ttggattagc
    12421 cgggttagtc atctatggtc tgctggcgat tggggcaatg tttctttttg tattcgaatt
    12481 ctacatcccc atctattcca tttctcagga atatgtattt tttctggata atactaacca
    12541 cctgtttatt tttgctctta ttttcactgt tctttatcat tttctggttc aaaactcaca
    12601 atatgaattg ctgttaaaag agcaaaatta tctgctcatt gccgataaag aaaagttcca
    12661 ttacctttcg catcacgatt cattaaccaa cttacccaac cgctcctact ttcatgctca
    12721 tcttcagacc ctattagaga atactgacac tacaaaggaa gccattactc tttattttat
    12781 ggatttggat aaattcaagc ccatcaatga cgaatacggt catgagttcg gtgatcagct
    12841 tttactttta accagcaagc gtttgcaatc ctgctttagg aaaaatgatt ttatagcccg
    12901 actgggagga gatgaattta ccgcgattat cgttcataaa ccccatgatc aaattgctga
    12961 ggctctaact cttagaattg aacaggaatt tgaacaacct tttgtcataa atggccagac
    13021 cattcattgc accataagcg ttgggatagc caattacccg cgcaatgcaa aaaatgcgga
    13081 agatttgtta aaatgggctg ataaagcgat gtatgacaat aaaaggaaaa aatatcaaac
    13141 gcttaaacaa acctcttagc caacactcct aatttaatca gtatgattgg gccttcattt
    13201 tatcaagcac aggatgcagc aatctgacat tcacttgaca attaactttt acaactttag
    13261 cattcattga ttaaatatca gagggtaaat gaatgactat tgaccgtata cgagcgctgg
    13321 ttagtgaaga ttttaacgcg gttaataatt taattattga taaaattcaa tctcaagttg
    13381 gcttaattca ggacttgtca caacatatag tcgaaagtgg cggcaaacgt ttacggcctt
    13441 tggttgtcct gttggcaagc aatgcttgtg gatacaaagg aaaagaccat atttctcttg
    13501 ccgctatggt tgagtttttc catactgcca ctttgttgca tgatgatgtg attgatgaat
    13561 ccactttaag aagaggtcga caaactgcaa atagtatttg gggcagtaaa gccagtgttt
    13621 tagttggtga ttatcttttt acccaatccg taaatttaat ggtagaggtt ggcattaaag
    13681 aaattctcaa tctactggcc aatacttccc accaaattac ctgtggtgaa gtcaaacaac
    13741 ttgccaatcg ccacaatccc gctttaaccc ttgatgacta tttcgacgtc atccgggcca
    13801 aaacagctct tttatttgct gcggctgcct gcataggtcc tatactcacg aatgcgccta
    13861 ttgagattcg aaatagttta tataattacg gtttacactt gggaaatgcc tttcaattga
    13921 ttgatgatgc cttggattat tgctcggatg ctgccacttt aggtaaaaac atcggcgacg
    13981 atttagctga tggaaaagcg actttaccac taatacatgc gttacaaaat ggtaatgcga
    14041 ctcaacaaca gcaaataaga gaaagtttaa tgaaaggcag tctaagccat ctgcctgata
    14101 ttttaattgc tattgagcaa accgaagcca taccgtttac gaaacgaatt gctgcccaag
    14161 aagtagactc agcactatct gccctggaaa tactgcctga ctcagtttat aaagaagctt
    14221 tagtcgattt ggccaaattt gccttggaaa gaaattatta acctgataat tgcatcgatt
    14281 ctaataatta cacaaccgat tgataataat ctggtggagt gtggcattta aagcaggcgg
    14341 ttttgcaatt ggctcctccc tgacacttcc ttattgctcc tttcctaacc cataagtcca
    14401 gcataggttg caatgcggtc agatccaagt gaaactccct actcaactgt tgagtgctta
    14461 ctaccccttc ccggtagata aaatcacgta tttgcagcag cattttgcct ccccatacca
    14521 tactggctat aacgaaatac agctaaaaca aaaagcagac tcagtgacat agccaaaatc
    14581 cagaccatac tttgttgagg atgatccaga aatttagcgc cttgataaaa aacaacagaa
    14641 gtagcgtaag ccacgacaaa tgaccaaaca atggaagtcc acataaatcg cttgtttgcc
    14701 tcctggcgaa tcactgccat agtcgaaaca caagggatat aaagcaaaac aaaaagcaaa
    14761 taagcatagg ctcctacagc tccatcaaaa cgccgtgaca taatgccata gacagattga
    14821 gataactcac tatcagctgc actggcagaa acagggttcc acaatgcaga gcctaattca
    14881 gacaaattgg cgggaataga cccaaaagca gctttgatgc ccccccaaaa atcaaagtga
    14941 gcagcagcta tttctcccac atggcctact tgggcataaa gtgagtttaa ggtgccaacc
    15001 actacctctt tagccagcat gccagttaat aaaccgacag tcgcgggcca gttgtcttga
    15061 tgaattccca ttggagcaaa taatggagta atccactgac ctattattga caataaagaa
    15121 tccgtattgg cttctccaga gctaatccct cctccccaag taatcgcatt taaccctccc
    15181 agaatcacac aaatgggaat aatgagctta cccgcacgat aaacaaaaaa tcgtaatcgc
    15241 atggtggttt ctctcagcaa tcttctgagt gagggtctat gataagcggg taactccaaa
    15301 atcaaaggag aagcatgccc cttcaacgta gatttgcgta aaatatatcc ggtaaataca
    15361 gccattaaaa tgccaatcaa atacaacgaa aaaacaacat tatgcccacc agaggggaaa
    15421 aaagccgcga caaataccgc ataaatagcc aaccgggcac tacaagacat aaatggactc
    15481 atcatcacag ttaataatcg atcgcgctcc gagtccaggg tgcgtgccgc cataatggca
    15541 gggacattac atccaaaacc cacaatcatg ggtacaaatg attttcctgg taatcccatg
    15601 gctcgcatcg ctttatccac gacaaaagca gctcttgcca tatagccaga agtttccaat
    15661 aaggacaaaa agaaaaacat cgcggcaatc acaggaataa aagtaatagt ggtattaatt
    15721 cctttgccta ccccattagc tacaagcgcg attacccaat ttggcgcatg gagctgttgc
    15781 aacaaccaac cactaccctg aacaaagata gtctctgtac taatgtcaaa aaaatcctgg
    15841 aatgctcccc caatattaat agcaaacaaa aacatcaaat acatcatcgc aaaaaatatg
    15901 ggcaaagcta aaaagcgatg caaaacaagt ttatctaatt tagcagtgaa gtgttcgctg
    15961 gcatcactat gctttttttg aaccaacgta acaatttcat ggattttttg atagcgagcg
    16021 tctgccagta acacatcaag attttgatcc gtttcttgaa gatttataag aaggttctcg
    16081 gtaaacgctt tctctcctat caaagtatcc ccttcggcaa gcctacggga gaaatagtag
    16141 gcaaaggaat ttttataccc ttttgcgagc agctgatttt ccaggtcatt caaaatttgt
    16201 tgtgctgcaa ctgataagga cagttttaga ggttttatct tttgggagca atgaagtaat
    16261 gattgttgaa gtgccggaat accaatgttt ttatgggcct gaatgggaat aacagaacac
    16321 cctaacaaac tctcgagttt ctctgtatca atagaaatac cccgatgctc ggcaatatcc
    16381 atcatgttaa gtgctacaac aacaggttta cctaactcaa aaagttggct ggttaaatac
    16441 aaatgtcttt caaggtgaca agcatctata acattaatga tgcaatcgta ttccaaatca
    16501 ataactgact gagcagcaat ttgctcatcc tggcttattc cttcggcatt cgcaaccaga
    16561 gaatagacac ctggcaaatc agtgatttca attaaatgtt ctccaagcaa aaattcacct
    16621 gtttttttct caacagttac tccgggccag ttgcctacgc gttgattcgc attggtcaag
    16681 gcattgaata gggttgtctt accacaattg ggatttccta ttaatagggc atgagtcata
    16741 gatgctccaa cactaattga ctcgcttcat ctttacgcaa agttaaaaat gttcccctta
    16801 cttcaatttg taccggacac cctaatggag ccatcctcac tacagaaaac tcaactccac
    16861 aagtaatacc aagggataaa agcctgcgtc gatactgcat atcagtcgca ccaaaactga
    16921 ccaatcgtac tttatcccct tgttttaatt cactaatctg cataattata aacaatacgg
    16981 tgattcattt aattaatttt aacacaaacg atatcgagaa tcattctcaa ttaagaaatt
    17041 ttataccaca ttacagcatt tcctttacca aacgccgagg atgacgaccc agatagtcca
    17101 ccgattgcat ctcaattaga cggctcaacg ttcttttaaa cgtttccttc atttcacctt
    17161 taacgtacaa ttcttctgca ggaacatccg ctgagataat gagcttaatg cccctatcat
    17221 acataacatc tataaagtga ataaacataa tcgcttgtgc tgtgtgactt tcagtaagct
    17281 gataaacatc actcaggaag acattatcaa agcgatcagc aatttctaaa taatccaatt
    17341 ggctacgagg aaaattacaa atcacatcaa aagcaaacca aatagaccgt gccccatatt
    17401 taatatatgg aatatcacga ttttgtactg ttataaaacc tttgcggtct gcttcccggg
    17461 ttattagagc aaactgtttt tccattgctt cttgtgtttg ctcatttaaa ggatataaat
    17521 aagcctctaa caagggttgt cggcctaaac gataatctcg tttttgatta agattcagaa
    17581 cctcacaatt ttttttaata atctcgatag ccggaataaa tctctctcta tgcaccccat
    17641 ttaaatacaa ctcatccgga cgagtattcg aagaaacaac caaaacaacg ccgtagtgat
    17701 gtagtgcttg taataattca gccaaaatca ttgcataagc cacatcgtga actaaaaatt
    17761 catcaaaaca cagtaaacga atcgatttgg ccatttcttt ggcgatatac tgcaaaggat
    17821 ttttttggcc ttgtaatcgt cttaattgag cgtcaatttg ctgcataaaa tgatgaaaat
    17881 gaaatctcgc ctttttttcc tcatcaatat gctgataaaa caaatccacc aaataagtct
    17941 tgccaactcc cacaggccca tagatataaa gccctttaat aggatgcttt tttctccagg
    18001 gaaaccaaga gtcattcttc ttctgtaaat cctcagcaag cctctgcatg tgttcaagga
    18061 tttctctttg ttcaggatcg ctatctattt cacctctata aatcgcggct tcatactgct
    18121 caatcaaact catgacaggc aaacctgatc acgaacagca tccatcagct tttcttttaa
    18181 tattaacagc ttaccatgaa aaaaatgacc cgtttcaaca aattcaatga ctggcaatac
    18241 ctgcgatgat tggctggcga actcactcac cagttcaaat ggcacaacct catcttcttt
    18301 gccttgtata ataaaccagg gatgaggagg tggctcaaat tctttgtaat cataatgatg
    18361 cacaggagga gctatcgtta ttaacagagc atgctcacaa taagacgccg ttctataagc
    18421 cacataagat ccgaaagaaa agcccgcaaa aatattttgg gcttgcggtt gctcagaatg
    18481 ccactgcctc acgagcttgc gcatatcgtc actctcacca ataccattat cataatggcc
    18541 tccagactgc cctacgcctc tgaaattaaa ccttactgaa ggaatatgca attccttaaa
    18601 agctcttgcc atcgtggtaa ctactttgtt attcattgtc ccaccttgta aagaatgtgg
    18661 atgaccaaga aaagcaacat aacgagtatc cacatcttca ggaacagtca gtacggcttc
    18721 cagtgggcca actaatcctt caagcattaa ttcatgctct ccgggagtat ttaatttgtc
    18781 ggtaaatagc ataataaccc tgccaattta aagataataa cccaaatcaa tatggattta
    18841 agcaaaagag ttaggctatt atagcaatgg atgcgttgac ccccaataga ccttttgtat
    18901 atctggttta ttttgttggc agcgactttt attgaccttc agaggaggac ttgatccatg
    18961 agacaggtac ctgtattacc accattctta tctattccag gctcattctc ctattcaggg
    19021 agatcggcat gaaaatccat gattttaaaa taaaaaagca ggaacaaaag aaaatttcca
    19081 tgttgacctg ttacgattac ccttcagcat gcatagtagc agaatcaaat atcgattgtg
    19141 ttctcgtggg tgattccgta gccatggcag tgcacggcca tcctacaacc attatggcca
    19201 ctatcgaaat gatggaatta catacccagg cagtagctcg tggtttaggc aaacagtttt
    19261 tgattactga cttacctttt ctcggacata aatcatctca aggtcataca gtagaaaacg
    19321 taaaaaggct gttacaggct ggagctcaag cagttaaaat cgagggcgct gataaagaca
    19381 cctgccaaac gatttcccat ttagttaatg ccggcatccc tgttatgggg catatcggct
    19441 taactcccca atccattcat caattaggcg gttataaaat ccagggaaaa aacagcgaac
    19501 aggcagaaac tctattacaa caggcagccg ccctggaaca agcaggctgc tttgcggtag
    19561 tcattgaatg tgttccacag ggcttggcaa aaacgattac cgattcgttg gttattccta
    19621 ctataggaat tggcgcaggc ccaggaactg atgggcaagt tcttgtctgg cacgacatgc
    19681 tgggtttaca gacttcattt aatcctaaat ttgtaaaaaa atattttaga gccaaagacc
    19741 attttattga agccttaaac agctatgtcc agcaagtaca acaaatgcat ttcccagcta
    19801 atgaacattc tttctaggaa aattatccat gcaaattttt cataatttaa atgagtggat
    19861 tcactttaga aataccctgt cccctgattt atccctgggg tttgctccaa ctatgggtaa
    19921 cttgcatgct ggacatgctt ctttgttttt agccagtagc aaagaaaatc actataccgt
    19981 ctccagtttg tttgttaacc cgacccaatt taataaccct gatgattaca agcattatcc
    20041 acgcacagtc gatgctgatc tcgaattaat gactcagaat ggagttgatt tttgtatact
    20101 ccccaatgaa aatgaaatct ataccgatgg ctatgcttat caagttcaag aaaaccggct
    20161 tgggcaattg atggagggga aacatcgacc ggggcatttt aacggagtcc tgaccatcgt
    20221 catgaaactc tttaatttgg tcaaaccaac ccgtgcctat tttggtgaaa aagattacca
    20281 acaactgttg ctaattcaag gaatggtaaa agcattgttc atggatattg aaatcaaatc
    20341 ttgccccaca gtaagagaaa aaagtggcct ggcctgcagc tcacgcaata acaggcttac
    20401 tccaagccaa agagaaatcg ctgatgaatt tgcaaaaata tttcatcaaa acaaatcctc
    20461 tgccatgata agcaaagaat tggaagcgct tggaattact gtagaatata ttgaagaatt
    20521 ccagggcaga cgatttgctg cagtaaaaat tggcgatatc cgtctcattg ataattattt
    20581 gctatgattc aaaccaattg gtgcgtgata acaggtggac cttcctcagg gaaaaccact
    20641 ttaattaatc atttggcaga gttgggatac aaaactgcgc cagaaatagc cagattttat
    20701 atcaaccagc tgctgtctca tgataaaaaa actctacaag aagtaaaaca tgacatcctt
    20761 tggttacaaa gaaaaattct caatatcatg ttaagacgag aaagaaacct gcctaaggac
    20821 gacttaattt tttttgaccg tggtaccccg gatagtatcg gctattttcg ttttcatcat
    20881 ttagatgagg ctcatgtggt tagagcttgt cggtatttac gctataaaaa aatttttttc
    20941 tgtcatccct tgcctgttgt ccctgatacc ataagagatg aagatgatgt caccgcaaag
    21001 aaaattagcg attacattta taatgcctat gttgacctta actaccctgt catagaattg
    21061 ccagcaacct caataaaaga acgcctggaa attattttat ctcatgttga tacctagaga
    21121 taaacctgtt ctaggtttta tttaaagcga ttgccgctgc aataaccgct gccttgtgtt
    21181 gaggcaagga tttatttgaa tactttaacc attcggggtg tgcctccact aaaccggtat
    21241 tgaatacccc tttctgaaac tcctctgatt ttatcatttc taaataataa ggaatggtag
    21301 tttttactcc aaacatgcgc atttcctcaa gagctcgacg tgcccgtcga agtaccgagg
    21361 gccaatcaaa agcccatacg gtcaatttgg cacactcaat aatacgccgt ccattaagat
    21421 aagcatccag aggtttttta ctaagacagt gagaatgaga agctcttttg acatgcaggg
    21481 caaatctgtc cacttcactg tgaatagtaa ctgctgctat ccccatctcc ttgcaagcac
    21541 gtactattcg cagggctatc tcaccgcgat tagccaccag aattttttta aacattaaaa
    21601 atctcttgta aaaactggct cattgcttgc catgaacggc gcgcagcaac ctcatcgtaa
    21661 attaatccta aatgttcatc atgcgcttga ggattagtaa aagcatgttt cacctgccca
    21721 tacatatgca tttgccaatc cacattagct tctgtcattt cctgacaaaa agcatgtact
    21781 tgttccggtt tcgccatagg atcatcataa ccgtgcaaag ccagaatttt tgctttaatt
    21841 cttccggatg ctagattctc aggtttatta agcaagccat gaaaacttac tgctcctcga
    21901 atatccgcac cactcctggc taaatcaagg gcacacaaac caccaaagca aaaccctata
    21961 atggctatgc gattgttatc gactaccgat attgatttaa tggcttccaa tgcggcatac
    22021 aaccgttccc taagcaaaga gcgatcgctg tacaagggtt gcattaatgc cattttttca
    22081 tcattgttag aaccaatttg tccttgccca tacatatcca aagcaaagcc tatatagccc
    22141 atctcagcaa gttgttttgc tttaaggcaa gcaaactcac ctcttccact ccaatcatga
    22201 gcgactatta cggcaggccg agattgttta attccctctt catgcgctaa aaatccgtgt
    22261 aactcaagat catcatgttg ataaatataa ttcgatgaat acataaaata tctcctaaat
    22321 tattttaaaa tagtctaggg caatatccgt aaaagtccag taattacatt agtttagcgt
    22381 aatgctattc tttgtctttt gttcctcgtg gctaagtaaa aatagtgtcg tccagtaaaa
    22441 ggatatcaaa ttgaattaat ctaatgctct tgcatgtttc gccaaaacat gagtccatga
    22501 gtcagtaaat atcaatatgt ccactaatta actcaaatta tggatttggt ctaaaataaa
    22561 aataaattat gtgaggaatg ttgccatgat gactgattcg ttaatacaca taagggattt
    22621 gttacatgct caattttgct accaaatgtt tattaccccc attcatcttc ctatcgataa
    22681 aaaatacaga gaatttgcca aaatggcctg tgaattcata gaaggaagac gggatgaaat
    22741 catacacaga gaatatcctc gtcatcatgt tttacatcga tttactcccg cgaacaaacc
    22801 aaatggaaaa aaaatattga ttacgcatgg ctggatgtct cgtgctgctt atatggttcg
    22861 cttaatcagg cttcttcata aagaaggata tgaagtctat gctatcgatt ttcctgccca
    22921 tggcgaagct aaaggaattc agcttccctg gacagatgcc attgctatta tcaaagaaac
    22981 gattaaccaa tttgggcctt tttatggatt ggttggccat tcttttggtg gttcaatgat
    23041 tttaaatact ttgaatctgg caggccaact accagaatgg caattaaatc acaagccgga
    23101 aagagccatt ttaattgcat cgccaacaca aatgcggact cctgtcaata aaattgccag
    23161 acgttttaaa ttaagcgggc aagcctattt acaacttcgc caattaatcc gtcaacaagc
    23221 atcggtagat cctgagagaa ttcgtttaag tcattttatt tcccaagccc ctgataccta
    23281 ttttctatgt atccatggag aattggatgc cactatcaac cccaaagaat cgataaattt
    23341 ttgcaaatat tacaaaaatg cccgcctatc tcttttacca gacgctgatc atgttagtgt
    23401 gttaatggat gagcgtgtcg aacaaatcgc tttggatttt ttaaatcctt aatatgacag
    23461 gggaattttg atccacctgg attacggctt tacctaatcc aggtggatcc agtagatagg
    23521 cagaccattg tatttgctga agcaatgtgc ttcacataca gaggaattgg cttcaaaatt
    23581 ttgtaattaa ccttccatgt attgacggat ttttttcatg gcattttttt caagttgcct
    23641 aacccgttca gcagagacac catatttttc tgccaaatca tgtaacgtga gtttttcttc
    23701 agcaagccag cgttgttgta aaatatcctg gctacgttca tccagttgtt ccatagcaaa
    23761 cagtaatttt tcacgcccct gatcgcctgt atcctcattc tctaacaaga cagcaggatc
    23821 atcgttcaca ttaaacaaat aacgctcagg cgctttgtaa gcatcatcat tatcatcaac
    23881 atctggcgca tcataagatg aatccataac atttaaacgt tgctccatca acaacacgtc
    23941 ttctcggcta acgcccaaat ctttggcgac agcgtcaact tcttcattgc tgaaccaacc
    24001 caatcgattt ttcatttggc gcaaattaaa aaataattta cgttgggcct tggttgtagc
    24061 aactttaact attcgccagt tacgcagcac aaactcatga atttctgctt taatccaatg
    24121 aacagcaaaa gaaacaaggc gtacgcccat cttggggtca aaacgtttta cggctttcat
    24181 caagcctaca ttaccttcct gaattaaatc acttaaaggc aatccatacc caagataacc
    24241 gcgggctaca cgaactacat agcgcaaatg cgcaagaact aattgtcgag cggcttctat
    24301 atctccttca gaatgaaaac gctcggcata tgcaatttcc tcttccaaag tcagcattgg
    24361 aatttgattt actcgatgga tataagaatc aagactacca acaggtagat tcattgcagc
    24421 aagttgcaac tgttgactca tactacttcc tccggtcatt aacactagaa tagtattaaa
    24481 atttacatcc tattaactga taggattcac caaaatgaat gctaagctat aaattttcaa
    24541 aaattgcaaa ttaagtaaaa cttcataact tagcactctc ttcatgatgt ttcaattttg
    24601 gtcattattt cagcgatcct tggcatattt ataaatcgct aaacctctta tataaaaatt
    24661 ttttacggtt tataaacaca caccaaactg aacaaatgtt gaccaaaacc aatacttcat
    24721 caactaatat tatacattat ttgtagggtt ctatagaagc caattgccgc ttaacagaca
    24781 acaaggctcc aagccacccg agtataatag caaataacac aagtagcaag atttgccgta
    24841 gtgacaaaca agctaaagga tagtgcattt gatagacatt agccagctga ttcactgcga
    24901 cgcccagggt tagaataaaa atattaacca gaaagatggc taaaagcgcg ccagctgccc
    24961 cataccaaac tccagaatat aaaaaaggac gaataatgta aggatctgtt gcgccaatca
    25021 gttttaaaat tttaatttct tcttgtctat tatgaatgtc tagacgtaag gtattgccaa
    25081 taataaatac tacagccaaa gctaataacg ccattaaagc atttgctgcc ttgccagaaa
    25141 aacccaagat agcgtgcaat cgattaatcc actccatatc cagtttcgcc aacgcgactt
    25201 gtggaaaagc ctgaagtttt cgagataata aatctaattt tgcaggagaa tctataacta
    25261 atgcgggaac tacttcaata actgcgggca aaggattttc aggaagatac cgcataatgt
    25321 cttgcatgcc ttcttgttga gttaattcgg ataatccatc tgctgcagat ttgatatttg
    25381 cctgaccaac tccttcagta gtgcgtactt tttcaagtaa cgatgtttgc tccgcttccg
    25441 gcaatgaagg tttcaaaaac agggaaatgt gcccccctcg ctgccaacga ttggttagtt
    25501 cacttaaatt gtcagtgaat acccaaaaaa gtgtaggtaa cgccaaagcg atggcaataa
    25561 caatgacggt catcatggtt gctatcggtt tgcggcagaa aagattcaag ctattggccg
    25621 cggcttgcaa atgataggca agaagagatt gtgatcgttt taacacagcc ttcctccttt
    25681 cagcatgacg atgcgatgtt tcattccggc tattaaagcc aaatcatgag ttgcaattaa
    25741 tatactaact ccaacctgat taaattgttc aaaaatcttc attatctcag aagaaagttt
    25801 tggatccagg ttaccggtag gttcatctgc caataacaat gcaggcttat gaactacagc
    25861 acgagcaata ccgactcgtt gctgttcacc aacagacagg tgaactggta acattttctc
    25921 tttactcaac aatcccacca tatccaaagc agcatgcacc cttttggcaa tcataggata
    25981 agcaactcct tgaatttgca agggcaaggc aacattatca aaaacagttc tgtcattcag
    26041 taactgagga gattgaaatg taatgcccaa ctgactgcgg tgagcagcaa catctcgctt
    26101 tttgagatga tttaatttca aaccattgac tgttaattga ccagacgtcg gccactcaag
    26161 aagagcaatc aacttgagca aggtgctttt gccagcacct gaatggccag taagaaagac
    26221 catttctcct ttttgcaaag aaaaattaac ttggcttaat gcctcaaagc cccccggata
    26281 acgcttacta acctggtcaa atgtgatcat catcgaatat tgcactaaca aattgcgctg
    26341 cgtcaaaggg cctcaaatct tcaatgccct cccctacccc taaataacga aaaggtatcc
    26401 ccaaatcatt cgctatagca aataatatcc cgcctttggc tgtcccatca agcttggtca
    26461 tagtaattcc agttaattgt actgcttcat gaaactgctt tgcctgagcc aaggcgttct
    26521 gcccaatact cgcatccaat accaacatgg tttcatgagg tgccttggga tcgagtttct
    26581 gcatgactct tttcactttt ttcaactctt ccattaaatt gctttgagta tgcaaacgcc
    26641 cggctgtatc ggcgattaaa acatctattt ttctcgcttt ggcagcctga agagcatcaa
    26701 aaattactga ggcactgtca gcgccggtat gctgggcaat aacaggtatg ccatttcttt
    26761 cgccccatac atgcaattgc tccaccgcag cagcccgaaa cgtatcgccc gcagctaaca
    26821 tgactttttt tccctgcttt tggaattgtt tggctagttt accaatcgtg gttgttttac
    26881 cggcaccatt gacaccaacc attaatatga caaagggaga accgtcttca gtttctatgg
    26941 aaagcggttt ctcttcctgg ctcaaaatcg tttgcaaatt tgtctttaga gtctgataaa
    27001 ctgcttcacc attagacagt tgctttcttg ccaagccttc atttagctgt ttcaatacgt
    27061 cttgagtggt gtccaaacct aaatcagcac taattaataa ggtctccagc tcttcgagta
    27121 actcctggct gatttctttt ttacctaaca gcagccgact tatgccttcg cccaattgat
    27181 gacgggtttt gctaaggcct ttcctaaacc gggcaaaaat actttctttg ggcgaaggct
    27241 ctttggcagt cagttcctgg ggttcttcaa gcctggattc catgctgctt tcggcatgat
    27301 cttgttgctc ttcggtatcc aaatttggtt cggaattttg atttcgttta aaccatttaa
    27361 tcattttagt gtacaattcc ttaaaaatat gaaattctat catcttttta gctttgaggt
    27421 gacattgtgc acaaattact catttctctt atgatgctac tctcatttca ggcttttagc
    27481 caagtccagg agtatacgtt aaacaatggt ttaaaagtac tggtaaaaga agatcatcga
    27541 gctccagtcg tagtatccat ggtttggtat aacgtgggtt cagcagatga gcctgtaggc
    27601 atcactggtg tttctcatgc catagaacac atgatgttta aaggaacttc caaatacccg
    27661 gtaggggtat tttccaaaac aattgctgca ttgggaggtc aggaaaatgc ttttacaaac
    27721 aatgactata ccgcctatta tgaaaaatta gatgctgatc atctggctac aagcttcgaa
    27781 ttggaagccg atcgcatgaa taatttactg cttaatagcg aggaattcgc taaagaaatt
    27841 aaagtcattc aagaggaaag acgtttacgt actgacaata acccgcaggc acttgctttc
    27901 gaacgttttc tggctacagc gcatcttaca gcaccataca atcacccggt gataggatgg
    27961 atgaatgatt tgaaacaaat gaaagtagag gatttaaaaa aatggtatga aagttattac
    28021 gcaccaaata atgccacctt ggtagtagta ggcgatgtga atcctgagaa agtacatgcc
    28081 ttggctgaac gttatttcgg ctcaatagcc aaacgtccca tagcgtctcg taaaccccag
    28141 caagaaccct cagcgctcgg gaaaaaaatg gtttacatta acgcgccagc gaaattgcca
    28201 ttactgttaa ttggctatac tgtacctagt gtaaaaactg ctaaaaataa ctgggaacct
    28261 tacgccttgg aaattatcgc cgggatactt gatgccgggg aaagtgcgcg ctttgccaaa
    28321 cacctggtgc gaggcaatca agtagccact ggtgcggaag cctattataa cctctattca
    28381 aggtaccaaa gccaatttat tgtctatggg gcgcctagcc aagatcatca aataaaagat
    28441 ctggaaaagg cattaattac ggaattggaa gccttaaaaa aagcgcctgt tagcaatcag
    28501 gaattacaaa gggttaaaaa tcaaatcata gcacaaaaaa cttttgaaaa agattctatt
    28561 tttggtcagg ccatggaact gggattgtta gaaacaatag gtctgggttg gaaaaatacg
    28621 gaaacataca ccaaagccat caatgaaata actcccgagc aaattcagca agttgcgcaa
    28681 cgatattttc aagaaaataa catgactgtt gcagaattaa agccaatcag gcaagaagag
    28741 gtaagaccat gagtgtttta agagctttta tcatttcgtt tagtgtaatt ttttctcaca
    28801 atctattcgc caattcattc aaaactgaaa aatggcagac taaaaatggg gtgcgagttg
    28861 tattttatca agcaatggaa gtacctatgc ttgatattag tttggcattt gctgccggct
    28921 ctgcctatga cagtaagtat tttggtttaa gcgcattaac aaccaatttg attaatcaag
    28981 gcaattccgg taaagacgca acgactattg cggaagcttt agcagacacc ggagcacaat
    29041 ttaatgccga aacgagtagg gatatggttg tccttagtct aagaactctt actagcaaag
    29101 aggctttgca gcaatctacc aaaaccttta gccaaattat tagtcatcct gattttccta
    29161 aagaagcctt cgcaagagaa aaagatcaac ttctcatggc agttgaacaa acagaagaat
    29221 cgccagatga tgtagccatc cagaactttt ttaaaacctt atatcaagag catccttatg
    29281 cccatcctgt acatgggaca gttgagtcac taaatgccat taaagaaaat caagtcattg
    29341 atttttataa aaaatatttt gttgctaaaa atggtatatt agttatggtt ggtgctattg
    29401 acagttctca agctcatcaa ttggcagaac agttgaccca ggatctacct gcaggagagc
    29461 cagcaccaac aattcccaag gcctctcagc tggccgatgc ggaaaaaatt aatgtaccgt
    29521 ttccttcctc acaaacagta gttcgcttgg gacagatagg aatagatcat cataatcaaa
    29581 attatttccc tttaatggtt gggaattata ttttaggtgg aggaacatta gtttccaggc
    29641 taggtactga agtacgagaa aaaagaggtt taacttacgg aatcgatagt caatttgtcc
    29701 ctatgctagg tgaaggaccg tttatcatta gtttatcaac caaaaactcc gaggccagaa
    29761 acgcactgca cattacccag gataccctga taaaatttat caaaaatggg cctaataagg
    29821 aggagctgac ttcagcgaaa caatatctga caggcagttt tcctctctcg ctaggaagca
    29881 ataccaacat tgcaaacctg ttattaagaa tggcgttcta ccatttacct gataattatc
    29941 tggataccta tgtcgctaaa atcaacgcgg taaccgatgc tgaaatcagg caagcattcc
    30001 agcaacaggt gaatcctgaa aaattactcc tggtaacggt tggacaatct tgaaacaggt
    30061 aatacggatt atcggaggat tatatcgggg aaaaaaaata catttccccg atgtagatgg
    30121 cctgagacca acaccagaca gagttcggga aacactcttt aattggctaa tgaatgatat
    30181 tagaaatgcc cgctgtctgg atgcttttgc aggtagtggc gcgttgggat ttgaagccta
    30241 ttccagagga gcgtctcatg tcgtttttat cgaacaatct cccaaagctc atgccagttt
    30301 gcaaaatacc attcgtcaat ttgatacccc caacttgcaa cttataaaaa ctgatacgtt
    30361 aaattatctt aaacaaagca ccgagcaatt cgatctgatt tttttagatc ctccctacgc
    30421 tctcaactat gttcctcaat gtcttgatca tatcataaca aataaccttc ttgttaaagg
    30481 aggtcttgtc tacattgaat catcatctcc tattgaagta aaatcagaac tttggaagca
    30541 attgaaatta aaacaagccg gtcaagtcat ttatggatta tttgaaaaac tctaacaaac
    30601 aatgtcttga caacaagcct tcctcctgtt tcaatcaaac taatatttcc aaatggtaaa
    30661 gaacattgaa tgaacaacaa taagattgtc ataatgttta ttttttcagc gcttcttgct
    30721 ggttgcgctg gaacaatgaa atttaaaaaa ccacccatca ataatccaag tgatgatgcg
    30781 actattaaat tagcagaagc tgccgtttca gtcagtgact ccatgcttga aatggcaaaa
    30841 gttgaaaaag taattactcc accaagcaaa gacaatacct taactatacc taatgcttat
    30901 aacttgcaag caagagccag tgtcgattgg tctggtccga ttgaagaatt gactgcgcgt
    30961 atcgccaaag cagcacattt caggtttcgt gttttaggca agtcgccttc agttccagtt
    31021 ttaataagca tcagtaccaa agatgagagt cttgccgaga tccttagaga tattgattat
    31081 caagctggga taaaagccag tattcatgtg tatcctaaca gtcaggtcgt agaattgcgc
    31141 tatgcgaaaa tttattctta gtttatctat tttgctatca gccctcctgg tagcctgttc
    31201 ttctcgtaat cattacgggg atactggctc attggcaggc cttcaagcga tggctgattc
    31261 aaaatacacc cgagctcaaa aaaaacaaaa aatgggtaaa attcgcgaga tggcccttaa
    31321 ggaaaccgct ttaagtgttg gcgcccaggc tgggttagcc tggagagcga aaataattga
    31381 cgagcaatta aacaaacagg caaggaatct tgatgcaatt tatgatttta attccctggt
    31441 cttagagcat aatattttac ctcctgtatt acttgaggga aggaatactt tgaatcttgc
    31501 agatgctcaa agtatccgtg tttcagatcg cacctataaa gtcgcaaaac aagctcattt
    31561 cattaccact ccacctacct ggcggcaata tttgtggatg gattatgtga aacctgaagc
    31621 tcccaatgtc actctactcc ccaaaaccaa ggctgaaaaa gaaatatggt gcatctatac
    31681 tgaaagagga tggaaaaatg gtatagatca ggcaaacacc attttggaag aaaacattgc
    31741 ccgcattaaa gaagatttcg gcggtatgat tctttataga aaattactgg ctatgaacat
    31801 ggtatctcct ccttatgttt ctcatactga cttgggcgta actggggatg gctcagaaat
    31861 tcatatcgat gacagggtct tacgcatcac ggcgttacct gaacttaatg tcaatagtgc
    31921 ggaatggaga gctgcggtcg ccaaagatga aaatgctctc gaacgtttca aaaatatgga
    31981 aaaactggca aatcaggcta aaattgttat aacgaacaaa tcctggcaac ctattatagc
    32041 accagtaagt taatgtctat ggataatatt aatttaatgc ccgatgagcc aactcgtttc
    32101 actccagtat tcatggacag aatgctggag catgcagaaa gcctgaatgc ttcagatata
    32161 acaatccaaa ctggcgaacc catttttgca gaggtttatg gaagactcct taaaattaca
    32221 aatcgacgtc tttccaatac agaattgggc gaccttatta attccattta tggcccgaat
    32281 gccacgacac aattactttc cggtaaagat atagatactc attatgagtt tcgacccaac
    32341 cgtggtgtac gctatcgata tagggttaac gcgactgcct gcctggttga aggccatgat
    32401 gccattcaga tcacattaag aactattccg accacaccac ccaaactcag taccatgaac
    32461 ctccctgata acattattga agcgattgct ccacaagaag gaatcgtgtt tattacaggc
    32521 gctaccggtt cgggcaaatc aacattgttg gcctctatta ttcgagagct tattgaaaca
    32581 tcagactcaa accgcaaagt attaacttac gaatccccga tagaattcgt ctatgatgaa
    32641 attgaaacaa tttccgctgt agtaagccaa tcagagattc caagacactt acccaacttt
    32701 gctgatggag taagaaacgc attaagacga aagccgagat taattatggt gggtgaatgt
    32761 cgtgatgcag aaaccatcag cgccgctctt gaagccgctt taacaggaca ccctgtctac
    32821 acaaccctgc atacttctgg tgttgcagaa acgatgcgac gtctggtcac gtcttttagc
    32881 ggagaagagc gtttgggaag aaccatcgat attcttgaaa caattaggct ttgcatttgg
    32941 caaaagctgg ttccaaccgt tgatgaaaga cgtgtagccc taagggaata tctggtattc
    33001 gatgaggaag tgagagatat tctattggag ggagacccca acgaagtgac ttcagccact
    33061 cgtaagttag tgaggcaaaa aggacagtta atgacttggg acgcaaaaat gaaatttgaa
    33121 cagggaatta tcagtgagcg agtttataaa ttaatcattg ctggagccaa agaatatcaa
    33181 caataatcaa aattaatctg cttgcacttg ccatagaaaa atcattgata taccgcatac
    33241 aaaacaatca agatcaagga ttcgattcag gatgtttcac aggaaaagct tataccatta
    33301 ttttaactta tttattttgt taattttagg ctcttctctc aattgggctt ccgtcaatca
    33361 gcacggcaca gattcgccaa ccctcaacat taaaatttta ggcattaatg attttcatgg
    33421 tcaaatcagc agtgggagag ccgtaaaaaa cgaacctgtt ggcggggcag ctgtcctggc
    33481 cgcttatctt aaagaagctc aattgggaat ggaagatcga tcaataatta ccatcatggg
    33541 tgatttggtt ggagcctccc ccccttcttc agggctgttg aatgatgaac ccagtatttt
    33601 attcattaat ggcttgggaa ataatcaatg caagcccgaa tcaagaatgg atcctctttg
    33661 caacatcgtt gccactgtag gaaaccatga atttgataaa gggcaaaagg ccatgtttga
    33721 tttaatttat ggtaccaata atccaccgac cgattcatgg atctcattac ccagttatcc
    33781 gggtgcatct tatccctatg tttctgcgaa tatcgttgac gccaagtcag aacaacctct
    33841 cttcccaccc tacataatta aacggataca taatactccc atagcattta ttggcgctgt
    33901 tttaaaagat gcggcggact ccatgtttcc tgcaaatgca gaaggcatta aatttttaga
    33961 tgaagcagac agtattaatc attacatccc ggaaattaaa gctaaaggag tcaatgttat
    34021 tattgtcctg attcatgaag gtgggaatca agttccttat gagggagata cccaagctgc
    34081 ctcagcagtt gaaggtcata ttaaagaaat agtttattcc cttggtgatg aaatcgacgt
    34141 tgttatgggt ggtcacaccc atcaatttct aaatgctttc cttccaaatc gcaatgggca
    34201 cgagatcttg gtcacacaag caaatagtta cagtgctgca tttgctgaag ttaccctcca
    34261 agtcgatacc agaaatcata cagtaaaaaa caaatcagcc aaaattatta caacttatgc
    34321 taatcgatgg ccaggaacaa atccggatac acaagcattg gaaattgtta aattagcaga
    34381 agataaaatt gggccaataa taaatagcca tgtaggcacc gcagaatatg atctgctaag
    34441 gaaacaaaat gaagatggag agtcgagttt aggaaatctg atcgcagatg cttatagaac
    34501 tgtaatgaat accgatctgg gcatcactaa ccccaatgga attcgtgatg atttaaaagc
    34561 aggggaagtt aactggggaa atatctattc tattttaccc ttttccaaca ggatagtcat
    34621 aattacctta actggaaatg acatttatga cttgctagag caacaatgga tgggctctta
    34681 tatcaatatg ttacaaattt ccgggtttgc ctacacctat gattcacatc aacctctggg
    34741 acataaaata attgaaatta gacatcaaaa taaaccattg cttagagaaa aaatgtatac
    34801 catagccact tcagattttc ttgccagcgg gaatggagtt ttttcagtta tgaaaagagg
    34861 aaaaataatc catattggtc aaagtgatca tgacacggta atcgcttata ttaaaagttt
    34921 acctcaaccc tttcacgcgt ctattgaggc aaggataaaa gaatcagaga ctggattaaa
    34981 aacaggagtt ggatagacta aattcatttt accgtcagcc cctgttttta ttatttatta
    35041 attaagtttt gctattattc tatgcattct aatccagcac atagtagagt ttgttttcgc
    35101 taagtcctca agttcattat gtaattgttt gatttcctcc cttttagcta taccaagctc
    35161 taaaagctga ggggtcaaat catcaagggc cattgaataa attagttttt ctttgttcgt
    35221 tctgagtaag ggttgaaata aagaacaatg ctcaatttca aaaccgcagt atgaaaactc
    35281 ttgctctaat cgatgcgcta tctgaaaatc tcgattgttt ttgctaaagc atgacctcgc
    35341 ccattgtgta aactggtcat aggagggtga aggaggagag caaaaggtag aatcaaataa
    35401 agagggttct tcacatacga tgaaaccacc agaattaaga caatttttca tttgaagaat
    35461 gacagagcgc gcatcagcca catgatgtaa aaccattcga caataaatca aatcaaattt
    35521 ttccccaaga tctgaaagat gattaacatc ccacacctta aatcgtatat tgctataagt
    35581 tctggttcgt tgttgagtat gatccagttg ctcctgactc aaatctatag taagcaaatt
    35641 gccctcccgg ccaattaatt tggctaattc gagggtcata tcaccagagc cacaccctat
    35701 ttccagagca gtcattgccg ctgaaggccc aacctcagaa agtaaatcca ggctgctctt
    35761 ggcatacaat tgatgttgca gagaaagacg ctctctcgcc ttttcattgg tatttagaat
    35821 gtaagtactg gtcataatat atccttatat ttgataaaaa ataactattg ttcttcaaaa
    35881 agggttacat ggctttccct ttgccacaaa ggctcactta aatattggct taagtgtttt
    35941 gcctgtaatt ggaacatctg gtttaagttt tctttagttg agctacccat atgcggtgat
    36001 aagacgacgt tatcaagttg ccttaaagcg aaaggaacct ccggttcata ctggtataca
    36061 tccaaagcag caccagcgat tgcatggcgt tgcaaagcat caatcagagc gttttgatcc
    36121 acaatgctgc ctcgcgcaac attaatcaaa tatccttctg cccctaaatt atctaaaacc
    36181 tgtttattaa ttaaatgttg tgtttcaaca ccaccggaac aacaaacaat taaaaaatca
    36241 ctaatagatg ctaaacttgc gacagttggg caatacaagt aaggactact tttttgactg
    36301 cgagccgtat aagcaatttt tagcccgaaa ggctctgcaa attgcgctat cttttcacca
    36361 atttgaccga atccaaccaa gcccaattgc tttcccaata aatggttgcc cagaaaacgg
    36421 ggctttttct caacccattc attatttcgg gtatagcgat catttagaat cacccttcgc
    36481 gacagtgcta acaataaagc gattgcaagt tctgctgtat catgaacacc tgcattaggt
    36541 tgtgaatgca gaataatatg attttgcttt aaaaaattaa tatcaatatt atccgttcct
    36601 attcccaggt gactaataat ttttaagtta ggaaattgca ttaaaaacga atggtcaatc
    36661 tgatcccata ccgttgtggc taaagctaca actcgggatt gatccactcc tgaagccatt
    36721 ttccaaccat gaataatgtt ccaatcagga atcagcatgg gaataattgg ctctagaaat
    36781 tggttggtca ggtaaacaga tggcttcatt aaaatgtcct gtttttcaga ataaagactc
    36841 cgtgcgttgt ccatgaacta taagctattt tctggtgatt tataatatcg ttaacaattc
    36901 tttctcatct tgctgacatg caaataaaaa caagcggtaa aatgagcgtt cgaaagtaga
    36961 gatatatagc gattattaca gttatgatac tatattcttt tactattgca tttcattatt
    37021 ccaaggagac gttaccatag agaggaatgc acagcaatat tttgaaagcg cgaaggaatt
    37081 gttcttgcca gtaatacctg tggaagagtt tgatggtttt atacttaaat tgggcaagaa
    37141 taactaccat ttttgcaaaa atgaaactcc ttttaataat agctgtagtg ccaatattgc
    37201 tattgataaa tattgtacct ctaaactatt ggaaaaagct ggtattcctg ttcccaaagc
    37261 catttcttta aactgtagtg aatttcaacg gaatgtgcat aaagataaaa ttgcacaatt
    37321 aaactttccc ttagtcatta aaccaataga tggttcgctg ggaatcggcg ttttatgtaa
    37381 tataaaaaca tgggaagaac tggaaagcca tttaacaaaa tatttttcct cttatcagtg
    37441 cctaataata gaagaattcc acggcaaact gaattcttac cgtgtattgg tttttaataa
    37501 ccgtatcctt ggtatagtcc aacgttatcc agccactgta actggcgata gggtacatcc
    37561 catacatgag ttaattaaac aggaaaattt aaacagaaaa agaatcaatg aagctttagg
    37621 tgaaattact ttagatgagg aaactcggat taaacttcaa gagctgggca taacagaaaa
    37681 ttacatcccc tcctttggag aacaaattgt tttatgctat acaagcaatg caacaagagg
    37741 tggatcctat gtcaccataa acaagaagat gtgcaagcaa aatcgtaaat tattgcttca
    37801 agcagccaaa atactcgact tgcaattagt tggaatagat gtggaatgct cagatattat
    37861 caatgttcct atagaacaat caaaaggggt catactggaa gttaaccaca aacccagtat
    37921 ccgaattcat gagttcccaa tgaaaggaac cccgcaaaaa gtcacaaaaa aaattatgcg
    37981 taatttcatc atccgccacc cgttgtctta cttatattct atctacaaca accaaccgac
    38041 cgctttttat gtccgcagtt ctattcttat tatcattatg ggattaattc ttttagccgt
    38101 gtggtaattc acctcaccta aaacttatgt catttcccaa tctttacttt caatacctgg
    38161 cagagagctt atttgataat aaatttattt ctgaaatcac tttgctcagg taaatgtagt
    38221 tcacttcatg ataaattctg ataattcact aacctcgatg aaagaaattt gagattaaag
    38281 tacttaatcg aacaatctat tgagcaggct cattttgcaa gtgctctttt aaagtttata
    38341 cttaataata atgaggttaa attatattac gaggtattaa atgaatcctg tatcgatttt
    38401 agatgcctca ttaagagatg gtggacatag aactaatttt cattttaagg atgatgattt
    38461 aaaacaaata ctacttcctc ttgataattc gggtattgaa tacattgaaa ttggctaccg
    38521 taatggttca cttcacccta ttgaaaatat agggaaagct ggaatgtgtc cccaagatta
    38581 tctcctgtat tgccaatctc taataaaaaa agcaaaaatt gcagtgatgg ttcatcctca
    38641 taatgtaaac gaacaggatc tgctcgagct aaaaaattgc ggtgtgaaat taataagaat
    38701 atgcatagca aaaggtgatc ttgtaaaagc tgtccctctg attaaactgg ctaagaattt
    38761 gaattttgaa acctcagtga acattatcca tatttcttat tatacggaaa gcgaactgga
    38821 tagtttgatt gaggaagtaa gcaaacacga accggatatg atctattttg ctgattctaa
    38881 cggaagctta tttcctgaca agataaatag cctatataaa aaatacacca gtcaatataa
    38941 agtttctttt gggtttcatg ctcatgataa ccttggtttg gcacaagcca atgctcttgc
    39001 agcggtaaac gccggggttc attttattga tgcctcactg gcaggaatgg gtaaaggaac
    39061 aggtaatcta aaaactgaat tttttatcgc ttatttacat gcaaacaata taaaaaaata
    39121 caatttggaa gatgtgttga cggcagctaa ttatgtaaga gatgccttga aaataggaca
    39181 agaacctatt gaaatgagtg aattcataag gggaatttct gatctgtcta cagcagacct
    39241 gaaaagctat aaaccaggca agctctagaa aatgcttata tttccaatat aggaatataa
    39301 ggctgggaca ccttgattcc ccaagatttg atgctccaga aagcaacgta ctttcagaat
    39361 taagctggca acatcataga tcttccagga gcaaatagag agtacaatgg caaaaaaaag
    39421 actattgatt ataggccctg tttcaactca aacctctagt ttgaagcact taagccaaaa
    39481 tttatctttt cttaatcctt attactccat tgaatgtctt gatcctcttg tcacgctgca
    39541 agaatgtccc gatgaggaat attatggaat ttggcaaaat caactaaagt cgctcatcaa
    39601 aatttatgat gcatttgttg gcttttcttt tggtggtgtt attcttcaac aatgtttttc
    39661 catattgagc ggggaaaaaa aacaaatcat tcttttttcc acaccaacat ttgcagattc
    39721 catcttaaga gaaaaacttg aaacagtcat ctctctctgt tctcaaaata agttggaaga
    39781 aggattgtct tcgctctatc aagcagttta cgcgccgcat tccattcctg atgcttcatt
    39841 gcagtattta aatagggaac ttgcgatcaa gaggcttatt tttggcttaa ccaaagtatt
    39901 gaaaacaaat tgtgtttcga ttttaaatca ttgtaatgtg aatcaccttc atttaatagg
    39961 tgaaaaatca caactggtta atatcaataa cgtgataaaa ccatcatgtg gccaattgat
    40021 tgtagttccc gaagcaagta tgcgagttct ggaaagcaac cccacctttt gtaaagaaat
    40081 tatatttaag gccctgaatc atgaaatcca ctgatcctat aaaaattgct gtattaccag
    40141 gtgacgggat tgggattgaa gtgactgagg ctacgcttcc tgtttttgaa gtccttgatg
    40201 tccctgcaac cctgagttat ggcgatattg gctgggaatt ttggaaaaaa gaaggaacag
    40261 caatcccctc cagaacatgg caattgatag ccgcctctga taccgttttg ctaggagcga
    40321 ttaccagcaa gccccaacgt gaagccaaac aggaattaag caaagcgctt agaaagagca
    40381 acccttatta cgtatctcct gtcattcaat taagacaagg tttggatctt tttgcaaatg
    40441 ttcgcccctg tttttccctc gatgaccaat caaagccgtt taatttttgc atcattaggg
    40501 aaaatagcga aggattgtat tgtggatttg attattttcc attaccaaaa gcaatacata
    40561 atctattagc agaaagccaa cattggcaaa caattccagc tgatgaagca agttgtgcct
    40621 taagattgca atcaaaaccc ggtttaaccc gtttatttga ttttgccttt aatcatgcta
    40681 tgcaaactgg catgcctaga gtaactttgg cagataaacc aaatgttctt agagaaagtg
    40741 gagaatttgc caggaaaatt tttgaaagca cagcccaaaa atacccgaaa atccaagcgg
    40801 acatactcaa tgttgatgcc gtagcattat ggttgataaa aagtccggaa aaatttggag
    40861 taatcgtcgc ggagaatatg tttggtgata ttttatccga cgtcggagca ggagttatgg
    40921 gaggattagg ccttgcaccc agtgccaata ttggtgataa agggagttac tttgagccag
    40981 tacatggtag cggaccaaga atgaaaaaaa attgtgccaa tccatcagct atgtttttaa
    41041 caattagcat gctgttaaat cattttggtt atcctgatcg agcaaaaaaa atagtcaatg
    41101 ccgtgatgca agtcataaag gaaaagcgtt ttattactta tgatctgggt gggcactcca
    41161 ctacgaccga tatggcgaat gcagtgattg agcgttgtgc gcgattagaa gcttcttgtt
    41221 tatcaacaga ttttagttcg gtccctaaag aaaatttaat tgagtcagat actatgagca
    41281 acttgcttca gcaattaatg cattgtaatt ctgcagaaat ttctgatgcc ctggacgctt
    41341 gtggaatcga aggcggatta ttgaatatca aaccgttatc acagggaatg aaaatggttg
    41401 ggcctgctta tactatccaa tatttaccca gagaaaaaaa agaaacagcc ttccataatg
    41461 ccgcaaacta tattgacaaa gttcccaaac attcggtcat agtcattgac aacaacggtc
    41521 aaactgattg caccgtttgg ggtgacctat taacacatac cgcattgaga aataacatca
    41581 tgggcacagt tgtacacggg gccgtgagag acgtcgaatc aattcgctcg acaaactatc
    41641 ctgtgttttg cacaggaatt tatatgtgct ctggtaagaa tcgcgtttac aaagcgaatg
    41701 agcaatgccc tttattcatt aatggagtta caataaatcc tggcgacatt gtctttgctg
    41761 atgacaatgg agtgttggtg atcccaaatg agcttcttca cgaggtagtg aataaagcaa
    41821 ttgccataag attaactgag gaacgcatta aagcagctgt agcatcaggt tccactcttg
    41881 agcaagctcg agaagattat tactatgaac aaccctggtt aggtattaat aaaaagagaa
    41941 agcaatagtg tttttgcctg aaaaactcaa attcatagac atacactacc atgcagcccc
    42001 tgatctgtat attcgtcgca gaaatgcaat tgaagcagga agattgtatc actctttacg
    42061 tggtgcagct gttttaaaaa gtcatattgg cagtactggt attcaggcaa ctattgccca
    42121 agaaatgggt ttgcccgttt ttccttccgt tgtgttaaat catatcaatg gaggaataaa
    42181 ttatagagta gttatccgtg ccttgagtga atatcaacct cttttttcgg gtaaaatgat
    42241 cgttgatttc ccaaccatca ccggtcgtaa actgcaatca aaactatcaa gagacttaac
    42301 tcacaatact ttaaaacaat atactggaca gaaagaaaca ctgtttggat caaaacaaca
    42361 gctacgcaaa gaggtgatag atattttaaa aatggcccgg gattacccca ttgttttaac
    42421 ttcaggtctt gcctcgagag aggaggttta taggctaatt gatgcctgca atcaatataa
    42481 tgttccttca ttattgttaa gccaaccgtc taatccttta accggtttaa aatttactga
    42541 actcaatgaa ttgattaaaa atgagtggtt atgggtggaa cagaccgcct taacttttat
    42601 ccttgagcac caagacaaac aagatttttc acaagtatta actcatctcc ctcgagtcat
    42661 ttacagctcg gatttagggc agaccagcca aatggatatt ccagagtggc tgcatttctc
    42721 aaaaagaata tttaatgaat tgagtttatc ggaaaaacga atggaagagc tattcctctc
    42781 caatgccatc acattactcg gcttataaag tcccaataga tccttgcata acccgcctct
    42841 ttcattctat tgctgcagtt ctcatttact gcatgtaaac cgcactcttc aaaacgtttg
    42901 cttcttgccc taaaacaaaa taatcagctg atgtaaatc
//
LOCUS       DASLOE010000009        42345 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.009, whole genome shotgun sequence.
ACCESSION   DASLOE010000009 DASLOE010000000
VERSION     DASLOE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 42345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42345
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.009"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1752
                     /locus_tag="U6309_000380"
     CDS             <1..1752
                     /locus_tag="U6309_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEN5503839.1"
                     /translation="SNLNSTKDILEKKVADRTKELEKILALTKSTLESTADGILVVDL
                     KGNIEFCNQQFLKMWGISPGFIDSHNDTETIQYVLNQLKNPEEFLKQIKELYNNPKQE
                     SFDELHFKDGKIFERYSQPHWLDNKIVGRVWSFRDVTLSTRLAYQANHDSLTGLPNRT
                     ALYNSLDHAINYCKRLNTSLSVLFLDIDNFKLINDSLGHDAGDILLYEFSRRLKGCIR
                     HSDTVARFGDDEFVLLLMTTEPKDVILVAQNILKAVAQPIKLPTQEVIVTTSIGISLF
                     PKDGIDASTLLKNSDTAMYLAKKEGRNNFQFYNNSINQQSLKRLEIQTQIHNAIKNNE
                     FFILYQPIYNLRSDELVGAEALIRWGHPAIGLLSPDEFIPIAEETGLINQVGEWVLRN
                     ACIQNKTWQLMGLPHISISVNVSWRQLKNSNFIEIAESVLKESRLDPQYLEIEFTEST
                     MIQKSTPIDSTLIKISQLGIKMAIDDFGTGYSNLSYLKTFPFNKLKIDRSFIQNCTTN
                     PNDASIVEAIIAMAHSLELKVLAEGVDTIDKAFFMHQHGCDEVQGFLYGLPVKADKFA
                     KLMQKTKIHHSKFINKT"
     gene            complement(1891..3021)
                     /locus_tag="U6309_000381"
     CDS             complement(1891..3021)
                     /locus_tag="U6309_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEN5503840.1"
                     /translation="MRGFLLALLLSINTAVLADELVGFAAFENGDYTTAYPHLMQAAK
                     EGNEEAMYLLGRMYQYGYGVTTNYEEARNWYQKAADKNNALAQLSLGFMYDTGKGVSQ
                     DFAEAFKWYMKAAEQGNPIAQRNIGLMYATGDGVAASDDKAFTWFKKAAEQGYSKAQV
                     NLGYQYMMGKGTPKDVKKAFEWYQKAAEQGDEKGEYSLGLLYTGQEGGISADDKAAFY
                     WFSQAANHGHVNAQTYLAYYYLKGYGVDADPVKAAYWYQSAAEKGQPEAQAQLGQLLL
                     TGTGVDKDYQQAAYWFGKSAHQGNPVGQAKLGYMYLAGLGVNKSLVKAYAWLKIAAEN
                     KNEEAAKQLKSLEAKLTEPEKLEAEKMIKDLGPLESKENYED"
     gene            complement(3379..4563)
                     /locus_tag="U6309_000382"
     CDS             complement(3379..4563)
                     /locus_tag="U6309_000382"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEN5503841.1"
                     /translation="MTDVYVVDVLRTPVGKAPRGVFKNTLPDDLLAHTIKCLIHRHQN
                     IDWQEIADVVIGCAMPEAEQGMNVARIASLLADLPQSVPAMTINRFCSSGIQSIVTAA
                     DTIRNQEAHLALGGGVESMSMVPMGGNKYTANPSIFNDEHVAIAYGMGITAENVAKRW
                     EITREQQDEFAIESHKKAIAAQERLDFNAEISPIEISIRYPDLKTSKVEIKKKLINKD
                     EGPRADTSYEAISKLKPVFALNGTVTAGNSSQTSDGAGVALLASEKALKQYDLKPLGR
                     LMSYAVAGVPPEIMGIGPVKAIPMALKRAGITLDQLDWIELNEAFAAQALAVIKDLDL
                     PREKVNPLGGAIALGHPLGATGTIRTATLLHGLRRTKGRYGMVTMCIGTGMGAAAIFE
                     SL"
     gene            complement(4579..6948)
                     /locus_tag="U6309_000383"
     CDS             complement(4579..6948)
                     /locus_tag="U6309_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HEN5503842.1"
                     /translation="MQEPFFIKKVAVLGAGVMGAQIAAHCVNAGIETLLFDLAGKEGP
                     KNSLVDKAIANLGKLKPTPLATPQTNELLQAKNYAENLEELSSCDLVIEAIAERIDWK
                     EDLYKRISPYLSDKTILVSNTSGLSINKLCEVLPAQHRSNFCGVHFFNPPRYMHLAEL
                     IPANSTSSNLLDYLETWLTEYLGKGVVRAKDTPNFIANRIGVFSLLTTLHHTLAMNMG
                     IDEVDALTGPLLGRPKSATFRTMDVVGLDTMQHVINTMQSQLTADPWHKMFILPDWLN
                     GLIKEGHLGQKSGQGIYRKAGKVIEVYDIQTGQYRPSRAEVSDELKAIMDIKDPEARM
                     KNLMTSNNKQAQFLAACFRDLFHYCAYHLEEIADNARDVDLAIRWGFGWSQGPFETWQ
                     LSDLHSVTRQIDESIQDNTALSSAKLPEWLYEIKEFYTREGAYSPGKNNYHARSQLPV
                     YHKQFFHDRVLKESHHPVHVIYENEGVCLWHLKDDVAVISFKSKANTIGQSVLDGIET
                     ALDIAERDYQGLIIYQQDASNFSSGADLRGVSKLLTDGTIQALEHMVEQFQRVAMRLK
                     YSSIPTVAALRGRALGGGCELMMHCDSIVAAFESYPGLVEAGVGVIPAGGGCKELAMR
                     SAHHAQQADLLTFILPVFQQIATAKVAASAVEARNMGYLSAADSILMHADEVLYASLE
                     KIKAMRAANYLPPMKKQRFKVAGIEGHARLQSGLVNWLEGGFISQHDYFLANELAKVL
                     CGGDVNHGTLVDEQWMLKLEREAFIKLANTSLTQARISHLLETGKPLRN"
     gene            complement(7068..8588)
                     /locus_tag="U6309_000384"
     CDS             complement(7068..8588)
                     /locus_tag="U6309_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEN5503843.1"
                     /translation="MDLLQRLNIKSVNPGAFSGHGWHSDNHVHTLESFNPSTGNKLAE
                     IATCTIDDYEQVMQRAEQAAQAWRKVPAPKRGEIIRQIGQALRENKDSLGSLVSLEMG
                     KSKQEGDGEVQEMIDIADFAVGQSRMLYGNSMHSERPNHRMYEQWHPYGIVGVISAFN
                     FPVAVWSWNAFLSAICGNVTIWKPSAKTPLCAVAVQHICNQVLKENNCPEIFGLIIPN
                     SHDVVEAMVDDKRIQLISFTGSTAVGKQVAAKVAARLGKSILELGGNNGIILDESADL
                     NLAIPAIVFGAVGTAGQRCTTTRRLFIHESKYQDVIKRLRHAYEQITIGDPLDTRNLM
                     GPLIDQQAVEQFKKAINRIKAAGGQIVYGGEVLKQAGSFVQPTLVCDVKNDWDIVQEE
                     TFAPILYVMSYRTLDEAIALHNGVPQGLSSALFTQNLKNAELFLSACGSDCGIANINI
                     GTSGAEIGGAFGGEKETGGGRESGSDSWKAYMRRQTNTINWGDELPLAQGIRFNLS"
     gene            complement(8598..9686)
                     /locus_tag="U6309_000385"
     CDS             complement(8598..9686)
                     /locus_tag="U6309_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEN5503844.1"
                     /translation="MYNVMITGAGKIGSLIACLLADSGSYQVHLADLEFNGSDVTRLL
                     TALPEIKTVALDVKDEQSTQAYLQKHNIIAVISSLPYFLNTHVAKAAKAAKAHYFDLT
                     EDTSVTEAVKAIAQNAETAFVPQCGLAPGFISIAANSLMQEFEKCHHARLRVGALPQR
                     ANNALHYSLTWSTDGVINEYGNPCYGIEGGKDVVMAPLEGLESIQIDGCEYEAFNTSG
                     GLGSLAELYAGKIQSMNYKTMRYPGHCKKMRFLMNDLRLNEDRGTLKRILENAIPKTY
                     QDIVIVYVTVEGIKQGELTEKSYVKKIYPEVIRGLEWSAIQVSTASGVCAVVDLVLGQ
                     GNEYKGLILQEKFKLSHVLENRFGKYYA"
     gene            9840..11375
                     /gene="lnt"
                     /locus_tag="U6309_000386"
     CDS             9840..11375
                     /gene="lnt"
                     /locus_tag="U6309_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEN5503845.1"
                     /translation="MKQAALIHSTIINTSTLNQYSKFVVVFLAGLLAPLGFAPFHLPG
                     LTILSLAILFSEFLQCTVKQGFTLGFLYGLGYFGFGVSWVIISIHDYGQLDYFLAGVI
                     TLLFIMYLSLFPGIVGYSFKLLSPKCNLLTCMLLFSTLWCIGELIRSKLFTGFPWLLT
                     GITQIDTPLRYIAPVVGVYGLGLICSFLGTLLVLIIKDNTIKRYYYLSVFILILIAPS
                     LGKNIHWTEIKKEPISVGAIQPNLSMRDKWDETLFWNLLKYYENKTDKLLGKNLIVLP
                     ESAIPLPASYVEDYLQKLHEKALKAKSSLILGILQPTDNDETNFYNSIISLGEATGAY
                     VKRQLVPFGEYIPKPFIPINRWLNLPEPNVVPGRSNQPLINIANHPIASLICYEIAYP
                     NILRSQMPAAQWIVSISDNGWFGHSLASYQQLQMAQMLSLLTGRYQILVNNDGLSSVI
                     NHQGTIVEGLPAFNSGMLQTDIFPANGITPWIIWSDYPAFVFCFIFVLFVLFFNIKRF
                     TEK"
     gene            11441..13912
                     /locus_tag="U6309_000387"
     CDS             11441..13912
                     /locus_tag="U6309_000387"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEN5503846.1"
                     /translation="MDNTYNPQEVEEQAQQYWHKKQSFNVTEDLNKEKFYCLSMFPYP
                     SGTLHMGHVRNYTLGDVIARYQRALGKNVLQPIGWDAFGLPAENAAIKNKIPPAEWTR
                     KNIAAMKEQFLRLGNAYDWKREITTCDPEYYRWEQWFFIRLFEKGLVYKKNAVVNWDP
                     VDQTVLANEQVVDGRGWRSGALVERKEISQWFIKITSYADELLSSLDSLDEWPAQVKQ
                     MQRNWIGKSIGTEIYFNVNNYPKRLKIYTTRPDTLMGATYLAVATDHPLAKEAASNNK
                     KVQEFLDSCQGIKIAEAELATMEKRGVDTGMTAIHPITGKELPIWVANFVLMQYGSGA
                     VMAVPAHDQRDWEFAQKYQLPVKQVIKPIDIEHDFNQSAYTEEGILINSNQFDNLLSS
                     KAIQVITNFLEENDAGKATINYRLRDWGVSRQRYWGTPIPMIICEQCGIVPVPDEELP
                     VVLPENVDFTGTGSPLTQCKEFVNVTCPKCGQDATRETDTFDTFVESSWYYARFACKG
                     QENAMLDDRAKYWTPVDQYIGGIEHAVMHLLYARFFHKLMRDEGLVNSDEPFKALLTQ
                     GMVLKDGHKMSKSLGNVVDPNHLINTYGADTARLFVMFASPPEQSLEWSDSGVEGAHR
                     FLKRVWAFSHQHRDMLIDINDSILSGNGHVDWKEAESRLKKSRHIVHQILAQATHDYD
                     RNQFNTVVSGCMKLFNEISDYSIETENDKFFIHSSISILLRLLAPITPHICHCLWQQL
                     GFDKAIIDAPWPKVDKSALKTDEVDYVVQVNGKLRAQFTASTDATEEELIAAAKEHAH
                     NFVVNHTIKKAIIVPHRQLINLVIG"
     gene            14013..14504
                     /locus_tag="U6309_000388"
     CDS             14013..14504
                     /locus_tag="U6309_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503847.1"
                     /translation="MTQIHKYLASLILIWLLTGCGFHLRGAIDIPEWLDNVSIISRDG
                     NKELVSILKSQLDGYKIDVNSDPSLAKYWLIINSSSLQQQIVSIGASTNPRQYQLILT
                     IEFMLQTPKGQIIKTPRKVNVSRQLTVNNDRILGSNEEQALLVSEMRRDAAIQIINRL
                     NRP"
     gene            14506..15531
                     /gene="holA"
                     /locus_tag="U6309_000389"
     CDS             14506..15531
                     /gene="holA"
                     /locus_tag="U6309_000389"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEN5503848.1"
                     /translation="MQINQQALTQLLQKKIHHLNILIGQDHYLIDEALNNIKSMLKKS
                     SECDEKILSIQSPEEWHVVLEETNNYSLFYQTVILTIFFDKKTIDTTGKKILSEYLKS
                     SNSHSYIIIRAPNLTAKQLQWLTHDEQALIIIAQPLSSEAMKRWINEQLKLNNISFDP
                     YVPELIYQYTKGNMLATAQVIEKITLSNTPGTLITPYLALEHLSDQCDFSLFELIDSC
                     LLGHSDRAIHILRQAANNKTEVTLVLWILTQEIRTLLQLLFLMQQKMDIKMASNQLKI
                     WTQRVNLYQSAVKRLNTLLLQQLHHYCQSIDDKIKSNSSNQAWNALENISLSLCLGQL
                     LGDACIA"
     gene            15519..16154
                     /gene="nadD"
                     /locus_tag="U6309_000390"
     CDS             15519..16154
                     /gene="nadD"
                     /locus_tag="U6309_000390"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEN5503849.1"
                     /translation="MHSIAIFGGTFDPVHNGHIKTSLAIQANFGFDSYYFLPCKSPAI
                     KPPSFASSEQRVEMLKLALKPYPDFKIDTRELDRDTPSYMVYTLQSFRQEYTDSSLTL
                     IIGYDGLLNLPQWYQWEKIISLANLLVINREEFFQQPVPKSVQTLLNQYRNDDKNILL
                     NHHAGSICLYNAGHYDISSTKIREQLKQHKDVKSNLPDLVYDYIKKQELYQ"
     gene            complement(16129..16662)
                     /locus_tag="U6309_000391"
     CDS             complement(16129..16662)
                     /locus_tag="U6309_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEN5503850.1"
                     /translation="MQLQWVDIAIVAIIALSVLTGLFRGFVKELVALCVWILAIWLGF
                     NYSQSLDPWLSSYIEEQSVRTAIGFIIILFATLFVGGVVNAILSFILKRAGLSGTDRT
                     LGMGFGFLRGVFIVALIMVAVKMTSLPYQEYSQKSKLYARFDPVVALLYSHLPEFIKQ
                     VKTVDKTENIIDTVPAS"
     gene            complement(16665..17435)
                     /locus_tag="U6309_000392"
     CDS             complement(16665..17435)
                     /locus_tag="U6309_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEN5503851.1"
                     /translation="MKLIMDEKVKHRLIGLAVIISLGAIFAPALIKKSSQSPESNFSV
                     NVKLPPKPLPPDVVITDEKEVFKTIKVAKTEIPPIPVEKKSINLAKAEPISTEEDNLI
                     RTSKAATLEAERENSIKSEPIKLAVNKAANATAKKVMTLAAAKPPVKRSPVVTAKVNT
                     NKKVSQIVAARKPAVKQDIYAVQLASFTQIANAQALVNRLRSKGYKANYLKSNSRNGV
                     VYKVVVGHSPVKKDVIKLKTQLASAMQLNGFVVNTGVS"
     gene            complement(17432..18718)
                     /gene="folC"
                     /locus_tag="U6309_000393"
     CDS             complement(17432..18718)
                     /gene="folC"
                     /locus_tag="U6309_000393"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEN5503852.1"
                     /translation="MHTPWSEWDLNHWLTHLETRNTQEIQLGLTRVLTVAQKLNLQRP
                     NCKVITVAGTNGKGSTVTALETIYHQSGFRVGTYTSPHLLVFNERIRINLIPIQDKDL
                     CKAFSVIEEARYPTNLTYFEMATLAALWYFKQHDLDLIILEVGMGGRLDATNIVDTDL
                     AIITTIDFDHQNFLGDTLELIGYEKAGILRKNKPFIYADLSPPKSVVEVALQLGASSY
                     LYGKDYFYQEEADHWGVTCQGKPFEQLTRPKIQLRSAAAAVMACHLMQNDLPVSYKNL
                     KKAMELIYIPGRLQLQKGKTDILYDVSHNPQSVRLLADTVKKINVKGQIHAVFSALKD
                     KDLYGLIMPLRDCVNRWYPAQLHNKRAASQEELLSVFREAEIFLDVCYNDPFTAFEMA
                     LKQASDDDLIIVYGSFFTVSHVMAAQHNLLEQKENQ"
     gene            complement(18699..19583)
                     /locus_tag="U6309_000394"
     CDS             complement(18699..19583)
                     /locus_tag="U6309_000394"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEN5503853.1"
                     /translation="MSWLKKLLPSRIRTETSQKKGVPEGLWVKCSGCNEVLYSTELAK
                     NFMVCPSCNFHHRISARLRIAQFLDESGQEEVAAGLEPQDRLKFRDSKKYKDRISQAQ
                     KATGEKEALIVVKGFLMQQPVVVSAFEFNFMGGSMGATVGEKFVRAVNVAATEKRPYI
                     CFTASGGARMQEGLFSLMQMAKTSAALAKFAETGLPFIVVLTDPTMGGVSASFGSLGD
                     IIIAEPNALIGFAGPRVIEQTVRQTLPEGFQRSEFLLEHGHIDMIVERKHLRATISEL
                     VSKLTHKGAESFFAHAME"
     gene            19994..21421
                     /locus_tag="U6309_000395"
     CDS             19994..21421
                     /locus_tag="U6309_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947070.1"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEN5503854.1"
                     /translation="MAQVINTNVASLTAQRNLGVSGNMMQTSIQRLSSGLRINSAKDD
                     AAGLAISQRMTAQIRGMNQAVRNANDGISLAQVAEGAMQETTNILQRMRELSVQAANS
                     TNNSSDRASIQSEISQLKSELERIAQNTEFNGQRILDGSFSGASFQVGANSNQTINFS
                     IGSIKASSIGGIATATGTEVAGAAATDITIAIGGGAATSINSSANFTGALNGQDASSA
                     YAKAAAINDAGIGGLSVTASTSGTQTVGAIGGTAGDTYNLTINGVAIYTNLNVATALT
                     NSDLRDAINGVSNQTGVVASLNGGNMTLTAADGRNITVTESGTGFTAGTDGLTVTGGA
                     FDGALRGTLSISAVDTIAIGGTVANIGLSANISKDTVGIDSLDVSTASGAQTAIKRID
                     AALNSVNSNRANMGALQNRFESTIANLQNVSDNLSAARSRIQDADYAAEMASLTKNQI
                     LQQAGTAMLAQANSLPQSVLSLLGR"
     gene            21504..21785
                     /locus_tag="U6309_000396"
     CDS             21504..21785
                     /locus_tag="U6309_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503855.1"
                     /translation="MSIEPVPSVNRAAQTDQIKIVEEASKPEIKNMVYDSALDAETKQ
                     ALDKATGLLQTIITEKISDKVLRKMPSDEYLQLLSLLDDIISGSIDKHV"
     gene            21848..23473
                     /gene="fliD"
                     /locus_tag="U6309_000397"
     CDS             21848..23473
                     /gene="fliD"
                     /locus_tag="U6309_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEN5503856.1"
                     /translation="MELSSPGVGSGLDVKNLVDAIVTAEITPSQVRHDKQLNSVNTEL
                     SALGQLKSYLTNLQTSLAKLSSLSQFYTMKSTISDPNYFSATLGTGATKNTYQIEIQK
                     LAQQHSLASSYMTNTGSGTITIDFGTYNSDKTTFTPNPSATSVNITITPGNDSLVAVR
                     DAINNTNSGITASIVQDSQGSRLTITSSKTGENYAMKISGGVASLNYDPTMGVNSLTE
                     TVAAQNSTVKINGLTLVQSSNQLNEAITGVTIDLKKAELGKVVTLTVDDNKDQLTGLI
                     NEFIKQYNDTMTFLTNLTGYNNETKQGGVFQGDPQFRNLKLNLNKWATTPLANQEGPI
                     RSLADLGIITNKQGLLEIKQDKFKTALDNHYKEIGALFAKTAKVNDTGINIKSINSTV
                     KAGHYDIVLTEFTPGVSMSGTIGGLSATSSNGITLTGSGDLSGLSINVFSGSAGARGQ
                     ITVTDGIAVQLSSLLDTYIEIKGDLDQRKDQLNNQAKQLNKIQNEIDIRRKSIETRYL
                     KQFTALDTLLSQLQSTSVFLSQQLANLPQLKLK"
     gene            23483..23893
                     /gene="fliS"
                     /locus_tag="U6309_000398"
     CDS             23483..23893
                     /gene="fliS"
                     /locus_tag="U6309_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEN5503857.1"
                     /translation="MRMKNLYSQISSQYKAIELQTRIETASPHELIDLLLQGARSHIA
                     TAQGNIQRNQIKEKGEHIGKAISIIEGLKMSLNHDKGGEIAENLLQLYDYVQQILLKA
                     NLKNDEDLLAQSNMLLAEVHQAWQAINSNAGNEL"
     gene            24216..24962
                     /locus_tag="U6309_000399"
     CDS             24216..24962
                     /locus_tag="U6309_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEN5503858.1"
                     /translation="MNTFKSSLASVICLLLSMQSAQAQMRYGETLCNLPEYFCMKIKS
                     GDSWDRLFPNIEEQDIVRRINRMNIRLKPGMIIAVPKNIERLTIYDVSPFPRYIEPSG
                     EKTIYVSQKKLAWGAYDEDGELLWWGPISSGVGKCKVIGGCSTPTGSFRIIRKQDIDC
                     VSTVFPRRANGEHGGALMPYCMHFFRGYALHGSYEVPGYRASHGCVRMFIEDARWLNE
                     EFIDLPGGGMRGTRVILDAPDAQENLDNLQ"
     gene            25203..26501
                     /locus_tag="U6309_000400"
     CDS             25203..26501
                     /locus_tag="U6309_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5503859.1"
                     /translation="MAHYNVQSESLNKASISLIMPWMVWGLGCLFYFYECLLQVSPSV
                     MSNELMRDFSVTSHTLGILSGIYFYSYAAMQLPGGVLMDYFGPQRLLTLATVVCALST
                     IAFGMTDNFFMACVARLMIGFGSAFAAVGTMKLAANWFPAQKFALLTGLMVTIGMMGA
                     IGGETPLALLIDNFGWRESMIIMGSVGLLLATLLILIARDTPKNFTPAIHQHPVEEER
                     LIPSLFTLIKNKQLWLVATYGGLMYMATPVFCGLWGVPFLMNKMLIAKSTAANFISLV
                     FVGWAIASPLWGLFSNRIGLRKPPMYIGCVGAMICSLLFIFAPITSSITMEILLFAFG
                     IFSAGFLPAFSVAKELCNKKYVATGLSFMNMMNMVGIALAQPLIGYILDKMWQGQMMG
                     TVRVYPLEAYYTALSILPIGMLVALLILPKIKETYCQSVN"
     gene            26540..27883
                     /gene="miaB"
                     /locus_tag="U6309_000401"
     CDS             26540..27883
                     /gene="miaB"
                     /locus_tag="U6309_000401"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEN5503860.1"
                     /translation="MVKKLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVI
                     LLNTCSIREKAQEKVFSQLGQWREYKAKNPHVLIGVGGCVASQEGSDIIKRAPFVDIV
                     FGPQTLHRLPALLNERLEKNKSVVDISFPEIEKFDHLPAPRAEGPTAFVSIMEGCSKY
                     CSFCVVPYTRGEEISRPFDDVLAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADL
                     ALLIHYIAAIDGIGRIRFTTSHPLAFSENLINAYAEVPELANHLHLPVQSGSDRILSL
                     MKRGYTALEFKSKIRKLRKIRPDIRLSTDIIVGFPGETDKDFQDTMDLVHEIGFDTSF
                     SFIYSPRPGTPAANLPDDTPMELKKQRLQILQNRLLMNAARYSESMIGSKQKILVTGF
                     SKKSSQQLSGRTECNRVVNFDGPPHLIGQFIDVQISDALPNSLRGRLLEKEMQPA"
     gene            27883..28848
                     /gene="rluD"
                     /locus_tag="U6309_000402"
     CDS             27883..28848
                     /gene="rluD"
                     /locus_tag="U6309_000402"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEN5503861.1"
                     /translation="MIEHIQQQLSVPRELHNQRVDSVLARLLPEYSRSLICNWIKTGA
                     VTLNNLPCKPKDKALGGDLITINVDHTIVNTDFNLCKPENIPIQIIYEDSEVIVVNKP
                     AGLVVHPGAGNKEHTLVNALLHHCPELQLLPRAGIIHRLDKDTTGLLIVAKNLISHTS
                     LIRKMQNREIKRHYLTLVHGYVISGGTIDTGFGRHPKNRLKMAVTDFGRQAVTHYNIN
                     KHYKDFTLLNVSLMTGRTHQIRVHMNYINHPVVGDQLYGGRIKFPPNADEAILSVLKQ
                     FKRQALHACSISFCHPVKNTEITLNALLPDDFELLLNTLDQHDEK"
     gene            28838..29563
                     /gene="pgeF"
                     /locus_tag="U6309_000403"
     CDS             28838..29563
                     /gene="pgeF"
                     /locus_tag="U6309_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEN5503862.1"
                     /translation="MKNRFANWPAPENITALSTTRLYGFSDGPYASNNLGLHVGDNQQ
                     HVLKNRQQLIELIDLPAEPIWLEQTHSTKCITVETEKTRDADAAITRLPKLPLVIMTA
                     DCLPILLCNRQGTEIAAIHAGWKGLYNGIIENTLKKLNSNRTDIIAWIGPAICKKCYE
                     VGEEVYLSFTNKYSLTRNAFEPVHSKWLADLPQIAEIVLNLEGVKSVFQSNLCTFELK
                     NEFYSYRRESQTGRIGTFIWINE"
     gene            29580..30956
                     /locus_tag="U6309_000404"
     CDS             29580..30956
                     /locus_tag="U6309_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEN5503863.1"
                     /translation="MITRIKLFIASLLVFFIPVADAAQDLTNMPSLAPVLKNAMPAIV
                     NVAVQGYLPNNVASGNADDDDGENSKQPSRIPEKGRKFESIGSGVIIDPKNGIIITND
                     HVIRNANLITVTLQDGRRLKARLIGGDSETDLAVLKIDAKNLKSLVIGDSDKLEVGDY
                     VVAIGNPFGLNSFGNSQSATFGIVSALKRSDLNIEGVENFIQTDAAINPGNSGGALVN
                     AKGELIGINTAIISPYGGNVGIGFAIPINMAKDVAQQIIKFGSIHRGLMGIFVQHLTP
                     ELAQSMGYAEDFQGALVSQVNQNSPAQLAGLKSGDIIVQINDTKITQATQVKTTISLL
                     RAGSTAKIKILRDNKPLTLDVEVTDIKKHEQKLQSNNPFLYGLALRNFEQESPPHGNV
                     VGVQVVGASETSAGWRAGLRPGDIIISANKTPVKDIKSLQAVAHEKKKQLLVQVLRGA
                     GALYLLII"
     gene            31264..31340
                     /locus_tag="U6309_000405"
     tRNA            31264..31340
                     /locus_tag="U6309_000405"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31298..31300,aa:Met,seq:cat)
     gene            31555..32691
                     /locus_tag="U6309_000406"
     CDS             31555..32691
                     /locus_tag="U6309_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEN5503864.1"
                     /translation="MFRINLIRYVALLFIITLNLDCISVAFSSQVIYGTQNAARVTGK
                     SLKKIYFLQLGAFTQKNNAQQLARKIQARTQYPVIISHSGNAFSVRVGPVNTQELHQL
                     STKLSQNSNITPIKHNSSPKQIPTIQSKLPILVKETSRPLPTHSVTKETIDGKGSWYV
                     AAKIGAQETTPDDQFTVNNDSGFPSPFDKDIYTNSSDSSLLLGIGGGYRFTNIILPAI
                     SVGVYYSHFFNMDINGQVIQYSLPEFTNYNYQWKVKSDLVLASVKANVFRWKNLMPFV
                     QGGVGASSNRASQYYENALPDVTPRISPNFANHTTTVFAYQLGAGLDWEIRPQWLLSL
                     GYEYSDLGKVRSGYGTDSWNRETLSSKNLTSNAGTFGFTYLFHG"
     gene            32744..35212
                     /locus_tag="U6309_000407"
     CDS             32744..35212
                     /locus_tag="U6309_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thaumatin domain-containing protein"
                     /protein_id="HEN5503865.1"
                     /translation="MWLPNGNLSNRTDLIFIKIMGERTNMKYSTRIIITTILLLFALL
                     GYAGKDPISWRVSGVFPAQSSVGQTYTVTYTFTNQLPFRLAKALIIERLASPNTEFNF
                     NDQCSGRLLNSGESCTVVVALNPLVIGLKTIQLTIAGYSKDRVPLPQASTLSTGQSLS
                     PIVSFVMDGFPSQLATGSVSSYKFTFTNNGAISATNLSVDVSQSLGAPNFTTTCNNTL
                     TPNGGVCEVDGTYTVTSGNPSIQQVTASLSYTGPSGSPVTAQTFTQVINPAAPLVGTL
                     VPPNYLPPLMIAGNQYTAQFLFTASGTVDITAPGSIACDQGGNDCSASLSSFNSSCTL
                     QTLTNAACQMTVTFTAPAANNPPRTYVLTATVPYSVGGNPQPAATAITAGTVLATLPT
                     ERTVTLVNECDFDVWFSLNGSQLGSSPNCPGTPCPNGTSCNTSTHKCFWSNPIPNIPV
                     APQAYLLTAGGGTNNVTIPVTNADPNIQWSGNISASTLCNGVTCQQAACDNAGGTAPC
                     NPGIGFTQPATQAEITMNLTTSDSYDVEVINGFHIPISMQPIYYQGVTTIPATPNNYN
                     CGEPGKDTTANGFGACDWSTATVPVIDQVPGNGFYWVTGGGQGCSITSANPGCPAMTL
                     CGLDSNFNQVCGNFLGYWSPDQVCGSSNVPAAVQSYFKCNQPLPTSTTPFYPSGAVLS
                     NLMLCSVPTGFTGPRYNTCYNAYPSSSPTDIAQCCGCVDWWNTAQTNNVAIGANPNTE
                     SCTQPGALQPQTNAQWNSFVQPMIQWMKRACPSAYIYPFDDKTSGFTCTNNLSGQPNS
                     TSYIIRFCPGNITGLPAGVNEGRG"
     gene            complement(35291..35605)
                     /locus_tag="U6309_000408"
     CDS             complement(35291..35605)
                     /locus_tag="U6309_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HEN5503866.1"
                     /translation="MVIYEVNLTIDPEIFVQFQAWLKKHVAEIIQFPGFMQACILKQE
                     KETKSGKEKLTVQYQLDNRDNLSRYLSELAPKMREEGIHLFQAKFSADRRIFEVREII
                     SK"
     gene            35994..37079
                     /gene="lbtU"
                     /locus_tag="U6309_000409"
     CDS             35994..37079
                     /gene="lbtU"
                     /locus_tag="U6309_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072401887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore legiobactin import outer membrane
                     protein LbtU"
                     /protein_id="HEN5503867.1"
                     /translation="MLCIRNYIRTRIILGILMLNVSVLAFADAGDSLFKNEKLTYDGH
                     LFFNATNSAIQGEKYLLNQQLSNIKMGSELQVTDWNKMKGLLIYNTLPTPVAPQFYFE
                     QFYDELQIESSNIFFAFGKKWLTFGNYKSDLIYKPLTKALGQTNEVTAIIGYDSLYYA
                     NASFFKPYSRISTSSLPLYYNLNTGVHNQSMDAGVSYLYSLAESQLFQYNKGFGGLLS
                     QSLKTRVPGFATYFNLKYKKTSTYLTYVTAITPFALEDMSYNNRRASPKALSIQNSYD
                     VNIKNFSFKIIGFYDYTFQALALGIPKQRLGLGLSATPFPYLGIQFQYSRDYSYGNNA
                     IASGINHFVNGRNTKTNNLALQTILNF"
     gene            37212..38954
                     /gene="lbtA"
                     /locus_tag="U6309_000410"
     CDS             37212..38954
                     /gene="lbtA"
                     /locus_tag="U6309_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore legiobactin biosynthesis protein
                     LbtA"
                     /protein_id="HEN5503868.1"
                     /translation="MAAVLNNSYKINNSLHSLLLEKDLLVSQSEMEHFISIAHQQCLD
                     RLQNAALREGLIDKEINSLHIHDYLDLLYHQQKKQKPEKTFDRWPLLKKELDESINNQ
                     ALALAYYHCWQQSLKQQAKNHSSLWSWLSEQHNKQQILTFLEQWGCIGHPYHPNFRAK
                     MGFIPEEVIRYSPEFNAQVDIPWLAIHRSVAFTSIDATSYSLLFAQHFAKEYQLWNYS
                     VQKQQLDPEQYLPLPVHPWQWNNKLQTLASVLINSKQLLITEAFQKTKPSMSFRTMMP
                     LDNKGPHLKLATAIHTTSALRTVSPASVSNSSPLSSWLRQLLTQYQSFEKPLFLARDL
                     AGINTTHPSISPQNKKHLAMIVRENPLELIDASQTLVPVAALYALSPVSQRALFIEII
                     EASGMEPELYFIEYSRCILECQLHLLLSYGVALEAQQQNTLVIFQSHRPVGLVIRDLG
                     GIRVCFNSLYETTPKPNLHPDSTISCHELSDLFNTFIYGNLLSNLTFCINCLHDNYNL
                     TKKNLWILLKQIANQLLQNFKKEINPLIYQKFQQHLFSLPWQHKSLLSMRLNQNQDNL
                     VFFSLDNPLSQDNE"
     gene            38947..40161
                     /gene="lbtB"
                     /locus_tag="U6309_000411"
     CDS             38947..40161
                     /gene="lbtB"
                     /locus_tag="U6309_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="legiobactin export MFS transporter LbtB"
                     /protein_id="HEN5503869.1"
                     /translation="MNKPVQKMMLSGQFLMILALEMTNPFIPLLIAHQSNTPVPSIVI
                     YNTLSLILPMLANILLTPIWGLAADRYGYKPMLMRASWALVLTQASMIFVNSVFCILI
                     IRLLQGAFAGFLVAMQTYALSITKWQNKSTQLSRLQSSKAIATSTAGFLGGLALSLTN
                     YQGLFGLATLICLGTTVAMHYKLPSPPKHQIKKRSQTLNHSYTSKSVFFFLCVLIMLT
                     QIAKFLPDPGFTLYLNKYCSNNLVLIGFLYSLPAMGMLCSSVWCGKQFDYCRSQPSLV
                     NQYLIRYSVLGAILMVIQASVDNFYLFGLIRILWGIVLAALLPALFALCSDRNLLPGY
                     ALGLANSFAKLGNLIGLLLGGLFAFYLPYPAIFMIIAGIYSLFAIFVYGYDRLNIRQM
                     TDFSNSYFNVKS"
     gene            40158..41318
                     /gene="lbtC"
                     /locus_tag="U6309_000412"
     CDS             40158..41318
                     /gene="lbtC"
                     /locus_tag="U6309_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038838172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="legiobactin import MFS transporter LbtC"
                     /protein_id="HEN5503870.1"
                     /translation="MKKNALLIVLLTWFLGNMDIHFLTPALPSLVDYFSVTPNTAQLT
                     ISLFLLGKALSMILWGLLSERYGRKPIFIGGLLLFILSNFLIALNHSILLFLVCRFLQ
                     GISVGATLLMGRAMINDTHNEQNATKYFAWLFTLAGLFICFLPFFGGLINSYWNWQIA
                     SLIIAAYGLLLLPLCRGLKETKPHYVKFPQLGHSIMVVFNHPLFVSYLLISALMMAGE
                     SAFNTSASFILIKGENFTSAQYGTIKTTMAIMHLLGTACCGLIAKYYHSIQLTKLGVR
                     FFIFAAFLMWLFVFSAENIYLTFIVPMVIYYFGTGFIVASATASAVRPFPQQMALALA
                     ITLFCQFNCSAFFSFITSMIGIQQVETFMFLLSLVSILSFLALLKLQRMEPC"
     gene            41462..>42345
                     /locus_tag="U6309_000413"
     CDS             41462..>42345
                     /locus_tag="U6309_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HEN5503871.1"
                     /translation="MDSNFKISIRVSIITLFVLLLSLVGFTIIGINYIAFNSVLNSSA
                     KDSIEQTSLLVKERFEIYLNPLNQDLIKITNAINNGIINPDDSKQFDKFLFESIRYNP
                     EIFMVYYGSTTGDFIGVDREQKGIIGLTHVINSVSPPVNVRYQLNEQGEIIGKKLLTR
                     HYDPRVRPWYQQAIKAGKPIWTNVYTFYVFDKSDFLIPGITGASPIYNKNKQIKGVIA
                     LDLTIDGLQHFIRELELTKNTMIYVINNESNIIAFRTPQNKKDIRGKKLTPEWIKKLN
                     IPLKKLDFNGFEPIIKSYT"
BASE COUNT        13240 a         8586 c         7595 g        12924 t
ORIGIN      
        1 tctaacttga attcaactaa agatatttta gaaaaaaaag tagcagacag aaccaaagaa
       61 ttagaaaaaa tcctggcttt aaccaaatca actttagagt caacagcgga tggtatttta
      121 gttgttgatc tcaaaggtaa tattgaattc tgcaaccagc aatttctcaa gatgtgggga
      181 atttctccag ggtttattga cagtcataat gacacagaaa caattcaata tgtattgaat
      241 cagcttaaaa atccagagga gtttttaaaa caaattaaag aactatacaa taatccaaag
      301 caggaaagct ttgatgaatt gcatttcaag gacggtaaaa tctttgagag atactcacaa
      361 ccacattggt tagacaataa aattgtaggg cgtgtttgga gttttagaga tgtgaccttg
      421 agcaccagat tagcctatca agccaatcat gattctctaa ctggtttacc caatcgtaca
      481 gctctataca atagcctgga tcacgcaatt aattactgta aacgattaaa cacctcatta
      541 tcggtattat ttctggatat tgataatttt aagttaatta atgacagcct gggccatgat
      601 gccggtgata tattattgta tgaattttcc aggcgtctta aaggatgcat acgtcatagc
      661 gatacagttg ctcgatttgg cgatgatgag tttgttttat tattgatgac cactgaacct
      721 aaagatgtta ttttagtagc gcaaaacatt ttaaaggcag tagcacaacc tatcaaattg
      781 cctacccagg aagtcattgt gacgacaagt ataggaatta gccttttccc taaagatggc
      841 attgatgcaa gcaccttact caaaaattcc gataccgcaa tgtacttggc taaaaaggaa
      901 gggcgcaata atttccaatt ttataataat tcgatcaatc aacaatcgct caaacgcctt
      961 gagatacaaa cacagataca caatgcaatt aaaaataatg aattttttat tctttaccag
     1021 cccatttata atttacgtag cgatgaatta gtaggtgctg aagccctgat acgttggggg
     1081 catcctgcaa taggactcct ctctccggat gaatttattc ctattgctga ggaaaccggg
     1141 ttgattaacc aagttggcga gtgggtgtta agaaatgcct gtattcaaaa taaaacctgg
     1201 cagcttatgg ggttaccgca tattagtatt tccgttaatg tctcatggag gcagttaaaa
     1261 aatagcaatt ttatcgagat tgcagaatct gtattaaaag aatccagact agatcctcag
     1321 tatttggaaa ttgaatttac cgaaagcact atgatacaaa aatcaacgcc cattgattcg
     1381 acattaataa aaatttccca gttaggtatt aaaatggcaa ttgatgattt tggtactggt
     1441 tactcaaatt taagttattt aaagactttc cccttcaata aacttaaaat tgatcgttca
     1501 tttattcaaa actgtactac taacccaaat gatgcctcca tagttgaagc aattattgcc
     1561 atggcccata gccttgaact caaagtattg gcagaaggag ttgacacgat tgataaagcg
     1621 ttttttatgc atcagcatgg ctgtgatgaa gtacaaggtt tcctttatgg cttacctgtt
     1681 aaagctgaca aatttgcgaa attaatgcaa aaaactaaaa tccaccattc aaaatttatt
     1741 aataagacat aaggttgttg tttcttttat tactataaag aaaatactac agactattca
     1801 ttaattaaac tagctcattt aaaccagtat tttaaacaca caacaaatta tcaagttaca
     1861 actataatct tctggattca gtttttaact ttagtcctca taattctctt tgctttctaa
     1921 tgggcccaaa tccttgatca ttttctctgc ttccaatttt tcgggttcgg tcaacttagc
     1981 ttccaaagat ttaagttgtt ttgctgcttc ctcattttta ttttcagcag caatttttaa
     2041 ccaagcataa gctttaacca agctcttgtt gactcctaat cctgctaaat acatataccc
     2101 caatttggct tgtccaacag gatttccctg atgtgctgac ttaccaaacc agtaagctgc
     2161 ctgttggtaa tctttatcaa cacctgtacc agttaataac aattgtccta attgtgcctg
     2221 tgcttcaggt tgtccctttt ctgctgcaga ttgataccaa taagctgctt tcacaggatc
     2281 agcatctaca ccataccctt taagatagta ataagccaaa taggtttgtg cgttgacatg
     2341 tccatgattg gctgcttgtg aaaaccagta aaaagcagct ttatcatccg cactaatccc
     2401 accttcttgg ccggtataca atagacctag actgtattcg cccttttcat ccccctgctc
     2461 tgccgctttt tgataccatt caaatgcttt ttttacatct tttggagttc ctttacccat
     2521 catgtactga tagcccaaat tcacttgtgc cttgctatag ccttgttctg ctgccttttt
     2581 aaaccaggtg aatgctttgt catcactggc agctacacca tctcctgtgg catacattaa
     2641 tccgatattt ctttgtgcta taggatttcc ttgctctgct gccttcatat accatttgaa
     2701 ggcctcagca aaatcctgag ataccccctt acctgtatcg tacataaatc ccaaacttaa
     2761 ttgagccaat gcattatttt tatcagctgc cttttgatac caatttctgg cttcttcgta
     2821 gttagttgtc acaccatatc cgtattgata catgcgtccc aataaataca tggcttcctc
     2881 attaccttct ttggcagcct gcattaaatg tggataggct gtggtgtagt cgccattttc
     2941 aaatgctgca aaaccaacta actcatcggc caaaacagca gtgttaatcg acaataatag
     3001 agctaacaaa aaacctcgca tacttgctcc attttatttc cttttaattt aagcgtagaa
     3061 taaagaagca acaaagcaag taattgttcg gttaaggcgt ggaaaaattt aaaaaaatct
     3121 caatcctgaa gaaaaaataa ttcaattagt taacagtttt ttctttaggc atagtcaaag
     3181 tcaataatat ccataattcg ttaaagtttt ttggaaaggg aaataaaccc cagagtgatt
     3241 tacatttaaa aatcccaaga gaaccaaccg atccccttga ttaacaattt tcttcccgta
     3301 tttataaaat aaacacggga agcattacaa ttcttgaaac tacaatagtg tatagaattt
     3361 aaatctggat tatatcttct ataacgattc aaatattgca gcagctccca ttccggttcc
     3421 aatacacatg gttaccatac cgtagcgccc ttttgttctt ctcaatccat gtagtaatgt
     3481 agctgttctt atggtgcccg ttgctcctaa aggatgtccc aaagcaatag ccccacctaa
     3541 agggttaact ttctctctag gtaagtcaag gtctttaata accgccaaag cttgggcagc
     3601 aaaagcttca ttcaattcaa tccaatccaa ttgatctaaa gtaattccag cacgctttag
     3661 cgccatagga attgccttta ctggtccgat tcccatgatt tcaggcggta ctcccgcaac
     3721 ggcatagctc attaaacggc ctaaaggttt taaatcatac tgttttaaag ccttctcact
     3781 ggctagtaat gccactccag ctccgtcact cgtctgagaa ctatttcctg cagtcactgt
     3841 cccgtttaaa gcaaatactg gttttaattt agaaatcgct tcgtaagatg tgtctgctct
     3901 agggccttcg tccttattta tcaatttttt ctttatttca acctttgaag ttttcagatc
     3961 agggtatctt atagatattt cgattggact tatttcagcg ttgaaatcga gtctttcctg
     4021 cgcagcaatt gcttttttat ggctttctat ggcgaattca tcctgttgtt ctcgagtaat
     4081 ttcccaacgc ttggcaacgt tttctgctgt aatccccatg ccataagcta tagcaacgtg
     4141 ctcatcatta aaaatggatg gattcgccgt gtatttattt ccacccatag gtaccatgga
     4201 catactttcg acaccgccac ctagtgctaa atgagcctcc tgattacgga ttgtatctgc
     4261 tgcagtaact atactttgta tgcctgatga gcaaaaacga ttaatagtca ttgctggaac
     4321 agattgtgga agatcagcca ataaagaagc gattcttgct acattcatac cttgttccgc
     4381 ttctggcatt gcacaaccta ttactacgtc agctatctct tgccaatcaa tgttttgatg
     4441 tctatgtatt aaacatttaa tagtatgagc caataaatca tcaggtaatg tgtttttaaa
     4501 aacacctctt ggtgctttac ctactggtgt acgtaatacg tcaactacat atacgtcagt
     4561 catcctaatt ctccttgctt agttgcgcag tggtttacca gtctctaaca gatgactgat
     4621 tctggcttgg gttaatgatg tattggctag ttttatgaat gcttctcttt ccaactttag
     4681 catccattgt tcatcaacca aggttccatg attgacatcc ccaccacaca acactttcgc
     4741 caattcattc gccagaaaat aatcatgctg tgatataaat ccaccttcca accaattcac
     4801 taaaccggat tgtaatcggg catgcccctc tatacctgcc actttaaatc gttgtttttt
     4861 cataggagga agataattag cagctcgcat ggctttgatt ttttctaaag aggcatataa
     4921 aacctcatca gcatgcatta gaatagaatc agccgcgctc agatatccca tatttctagc
     4981 ttccaccgca cttgcagcta cttttgcagt tgctatttgc tgaaaaactg gcaatataaa
     5041 agttaataaa tcagcttgtt gggcatgatg tgctgaacgc atagcgagct ctttgcaacc
     5101 accaccagca ggtataactc ctactccagc ctcaactaaa ccgggataag attcaaaagc
     5161 agcaacaata gaatcacaat gcatcattaa ttcacaacca cctcctaatg ctcgacctct
     5221 taaagcagct acagtcggaa ttgaactata ctttaagcgc attgctaccc gttgaaattg
     5281 ctctaccata tgttcaagag cttgtattgt tccatctgtt aataatttgg agacgccacg
     5341 tagatcagct cctgaactaa aattggatgc atcttgttga taaataatta atccctggta
     5401 atccctttct gcaatatcca aagcagtctc tatgccatcg agaactgatt gaccaattgt
     5461 attggctttg cttttaaaac taatcacagc cacatcatct tttaagtgcc ataaacaaac
     5521 accctcattt tcataaataa catgaacagg atgatgtgac tcctttaata ccctgtcatg
     5581 gaagaattgt ttatggtaaa caggtaactg acttctggcg tggtaattgt ttttaccagg
     5641 cgaataagcg ccttctcgtg tgtaaaattc ctttatttca tataaccatt caggtagttt
     5701 tgcgcttgat aatgccgtat tatcctgtat tgattcatcg atttgtcgag tcacactgtg
     5761 taaatcagaa agctgccagg tttcaaatgg tccttgcgac catccaaatc cccagcgaat
     5821 tgccagatca acatctcttg cattatcagc aatttcttcc aaatgatacg cacagtaatg
     5881 gaataaatct ctaaagcaag ccgccaaaaa ttgagcttgc ttattatttg atgtcattaa
     5941 attcttcatt cgagcctcag gatctttaat atccattatc gctttcagtt catcactcac
     6001 ttctgctcga gagggtctat attgaccggt ttgaatatca taaacttcta ttactttacc
     6061 tgcttttcgg taaattcctt ggccagattt ttgccctaaa tgaccttcct taattaaacc
     6121 attcaaccag tctggcagaa taaacatttt atgccatgga tcagcagtta attgagactg
     6181 catggtatta ataacgtgtt gcatagtatc caaaccaacc acatccatgg tacgaaaagt
     6241 tgcagatttc ggccttccta ataatggacc agtcaaggca tccacttcat caattcccat
     6301 attcatggct aaagtatgat gcaaggtagt taatagtgaa aagactccta ttcgatttgc
     6361 gataaaattg ggagtgtctt tcgctcttac aactcctttg cccaaatact cggttaacca
     6421 tgtttccaaa taatcgagca aattagatga tgtgctattg gcaggaatta gttctgccaa
     6481 atgcatgtaa cgcggtggat taaaaaaatg gactccacaa aaattggatc tatgctgcgc
     6541 aggtaatacc tcacatagct tattgatact tagccctgat gtattactaa ctaaaattgt
     6601 tttatcagat aaataaggcg aaattcgctt gtataagtcc tctttccaat ctatacgttc
     6661 cgctatcgct tctatgacta aatcacaaga agataactct tccaaatttt ccgcataatt
     6721 ttttgcttgt aataactcat tggtttgggg agttgcaaga ggagttggtt ttagtttacc
     6781 taaattggca atagctttat cgaccaagct gtttttaggt ccttctttgc cagctaaatc
     6841 aaacagtaaa gtctctattc cggcattaac gcagtgtgct gcaatttgag cacccatgac
     6901 gccagcccct aaaaccgcaa cttttttaat gaaaaaaggc tcctgcattg ttattatcct
     6961 cttgttaata atcgattgat gcctctatga gtgagattta ctggtaacag gcacttacca
     7021 ttggctaaaa ggagcaataa tctgttgatc ttgctccttt ctacatctta acttaaatta
     7081 aaacgaatac cctgagccaa aggcaattca tctccccaat taattgtatt ggtttgtctc
     7141 ctcatataag ctttccagct atctgaacct gattcacgtc cgccaccagt ttctttttca
     7201 cctccgaaag cacctccaat ttcagcccct gaagtgccaa tattaatatt ggcaatacca
     7261 caatcacttc cacaagcact gagaaagagc tctgcatttt ttaagttttg ggtgaataag
     7321 gctgaagaaa gcccttgagg aacgccatta tgtaaagcaa tagcctcatc cagtgtccga
     7381 taggacataa catatagaat aggggcaaaa gtctcttcct gcacaatatc ccaatcgttt
     7441 tttacatcac aaactaaagt aggctgcaca aaggagccag cttgttttaa tacttctcca
     7501 ccataaacaa tttgacctcc tgccgctttg attctattta ttgctttttt aaactgttca
     7561 acagcttgct ggtcaatcaa aggacccatt aaatttcgtg tatcgagtgg gtcaccaata
     7621 gttatctgct cataagcatg tcttagcctt ttgataacat cctggtattt tgattcatga
     7681 ataaacaggc gtctcgtagt agtacaacgt tggccagcag tacccacagc accgaatact
     7741 atggcaggaa tagccagatt caaatcagca gactcatcga ggatgatgcc gttattacct
     7801 cccaactcca gaatgctttt acccagtctg gcagcaactt ttgctgctac ttgctttcca
     7861 actgcagtag agcctgtaaa ggatattaac tgtattcgtt tatcatcaac catggcttca
     7921 accacatcgt gagaattggg aatgatcaag ccaaatattt caggacaatt attttctttc
     7981 aagacctgat tgcaaatatg ttgtacagcc actgcacaga gaggtgtttt tgctgatggc
     8041 ttccatatag taacgttgcc acaaatagca gataaaaatg cattccagga ccatacagca
     8101 acaggaaagt taaatgctga aatcacccca acaattccgt acggatgcca ttgctcatac
     8161 attcggtgat ttgggcgttc tgagtgcatg gaattaccat acaacatacg cgattgccct
     8221 acagcaaaat cagcgatatc aatcatttcc tgaacttcgc catcaccttc ctgtttggat
     8281 ttacccattt ccagcgatac cagacttccc aagctatctt tattttctct tagtgcttgc
     8341 cctatttgcc tgatgatttc accccgcttt ggagctggca cttttcgcca ggcttgcgct
     8401 gcttgctccg ctcgctgcat aacctgctca taatcatcga tagtgcaagt tgctatttca
     8461 gcaagtttat tcccagtact gggattgaag gattccagag tatggacatg gttgtcacta
     8521 tgccaaccat gtccactaaa agctccgggg tttactgatt taatatttag acgttgcaat
     8581 aaatccataa gattctccta ggcataatat ttgccgaagc gattttccaa cacgtgggac
     8641 aatttaaact tttcttgcaa aataagccct ttatattcat ttccctgtcc caataccaaa
     8701 tcaacaacag cacatacgcc cgaagctgta ctgacctgaa tagctgacca ctctaaacct
     8761 cgaattactt cagggtaaat tttcttgaca taactttttt cagttagttc gccctgctta
     8821 ataccctcaa cagtaacata aactatcacg atatcctggt atgtttttgg aattgcattt
     8881 tccagtattc tttttaacgt ccctcgatct tcatttaatc gtaagtcatt cattaaaaag
     8941 cgcatttttt tacaatggcc tggataacgc atggttttgt aattcatcga ttgtattttt
     9001 ccagcataaa gttcagccaa acttcccaaa ccaccggacg tgttgaacgc ttcatattca
     9061 caaccatcaa tctgaattga ctccaatcct tccagtggag ccataaccac atctttacca
     9121 ccttcaatac cataacaagg attaccatat tcattaataa ccccatcagt agaccaggtt
     9181 aatgaataat gaagcgcatt attagcacgc tgtggcaatg ccccaactct tagtctggca
     9241 tgatgacatt tctcaaattc ctgcatcaaa ctattggccg caatactgat aaagccagga
     9301 gctaatccac attgaggaac aaaagctgtc tcggcatttt gagcaatagc ttttactgcc
     9361 tccgttacag aggtatcttc cgttaaatca aaataatggg ctttggcagc tttggcagcc
     9421 ttggcaacat gagtatttaa aaaataggga agactcgaaa tcactgcgat aatattgtgc
     9481 ttttgcaaat aggcttgagt ggattgttca tctttaacat ccaatgcaac cgttttaatc
     9541 tcaggcagcg cagttaataa tcttgtcaca tcagagccat taaattccag atcggccaaa
     9601 tgaacctgat aagacccact atcggcgagt agacaagcaa ttagactgcc tattttccca
     9661 gcaccagtaa tcattacgtt gtacatacaa tccttctcct ttgtaaaact tgataatcct
     9721 acacaaatta tcagagatta gatagatcca agacatgatc aatacagcaa ttcttgatat
     9781 cacttgacca attagtctta ctcatttatc ttgaccactt taatcagtct agttgtgaca
     9841 tgaagcaggc cgctttgata cattcgacaa taataaacac atcaacacta aaccaatatt
     9901 cgaagttcgt tgttgtattt ttagcaggtt tattagcccc tttgggattt gcacctttcc
     9961 atctgccagg attaactatt ttgagtctgg ccattttatt ttctgaattt cttcaatgca
    10021 cggttaagca gggatttact cttggtttcc tatacgggtt aggatacttt ggcttcggag
    10081 tatcatgggt gattatcagc atccacgact atggacaact tgattacttt cttgctggcg
    10141 ttatcacctt attgtttatc atgtatcttt ctctgtttcc tggaatagtc ggttattcct
    10201 ttaaactcct ttcacccaag tgtaatttat taacatgcat gcttttgttt agcactcttt
    10261 ggtgtatagg tgagctcatc cgttccaagc tttttactgg attcccctgg ctattaacag
    10321 gtattaccca aatcgataca ccattgagat atattgcacc agtagttggt gtttatgggc
    10381 ttggattaat atgctctttt ttgggtaccc tactggttct gataattaaa gacaatacga
    10441 ttaaacgata ttactacctt agcgttttta tcttgattct tattgcacca tccctgggga
    10501 aaaatatcca ttggacagaa atcaagaaag aacctatttc tgttggtgca atccagccta
    10561 atctctcaat gagagacaag tgggacgaaa ctctcttttg gaatttatta aaatactatg
    10621 aaaacaaaac agataaactg ttaggaaaaa atttaattgt tttacctgaa tcagcgatac
    10681 ccctaccagc aagttatgtt gaagattacc tacagaaact gcatgaaaaa gcattaaaag
    10741 caaaaagcag tttaattttg ggaatcttgc aacctacaga taatgatgaa accaattttt
    10801 acaattccat cattagcttg ggagaagcta ctggagccta tgtgaaacgc caattggtac
    10861 catttggaga atatatacca aaacccttta tccccatcaa tcgttggtta aatcttccag
    10921 aacctaatgt tgttcccggc agatcgaatc agccattaat taacatcgct aatcatccca
    10981 ttgccagcct aatttgctat gaaattgctt acccaaatat cttgagatca caaatgcctg
    11041 cagctcaatg gatagtctct atcagtgata atggctggtt tggccattcc ctggcaagct
    11101 accagcaatt acaaatggct caaatgcttt ctttgcttac aggacgatac caaatcctgg
    11161 tgaataatga tggactctcc tcagtaatta accaccaagg aactattgtc gaaggtctac
    11221 cagcttttaa ttcaggtatg ctccaaacag atatttttcc tgcaaatggc atcactccct
    11281 ggataatttg gagtgactat cctgcctttg ttttttgttt tatatttgtt ctttttgttt
    11341 tattttttaa tattaaacga tttacagaaa aataatctat tgctggcaaa ggcaagaggg
    11401 gttatcctta gcagttccgt gttattaaat ttattggcct atggataata cctataaccc
    11461 ccaagaagtt gaagaacaag cacaacaata ttggcataag aagcaatcat ttaatgttac
    11521 tgaagattta aacaaagaaa agttttattg cttatccatg tttccctatc ccagtggcac
    11581 attgcatatg gggcatgtga gaaactatac tctcggtgat gtcatagccc gatatcagcg
    11641 ggcattggga aaaaatgtcc tgcaacctat aggatgggat gcttttggcc tacctgctga
    11701 aaatgcagcc atcaagaata aaattcctcc tgcagaatgg accaggaaaa atattgctgc
    11761 catgaaagag cagtttttac gcttaggaaa tgcttatgac tggaaaagag aaattactac
    11821 ctgtgatcct gaatattatc gctgggagca atggtttttt attcgtttat ttgaaaaggg
    11881 cttagtttac aagaaaaacg ctgttgttaa ttgggatcct gttgatcaaa cagtattagc
    11941 taatgaacag gtagttgacg gtcgtggctg gcgttcgggt gctttggttg agcgcaagga
    12001 aatttctcag tggtttatta aaattacctc ctatgctgat gagcttttaa gctctttaga
    12061 tagcctggat gaatggcctg cacaagtaaa acaaatgcag cgcaattgga ttgggaaatc
    12121 cataggcaca gaaatttact tcaatgtaaa caattatccc aaacgattga aaatttacac
    12181 tacccgccca gatacattga tgggagcaac ctatcttgct gtggcaacag atcatccatt
    12241 agcaaaagaa gctgcttcca acaataaaaa ggttcaagaa ttcctcgata gttgtcaagg
    12301 aattaagata gctgaagcag aactggcaac tatggaaaaa cgaggggttg acactggaat
    12361 gactgcaatc caccctataa cgggtaagga gctacccata tgggttgcta attttgtttt
    12421 aatgcaatat ggttctggtg cagtcatggc agttcctgca catgatcagc gcgattggga
    12481 gtttgcacaa aaataccaat tacctgtcaa acaagtaatc aagcctatag acattgagca
    12541 tgactttaat caatcagctt atactgaaga aggaattctg attaactcaa atcagtttga
    12601 taatctactc tctagtaaag ccattcaagt catcacaaat tttctggaag aaaatgacgc
    12661 tggcaaagct accattaatt accgtttaag agattggggc gtttccagac aaagatactg
    12721 gggaacccct atccccatga tcatctgtga acaatgcggt attgttccag tccctgatga
    12781 agaattacct gttgttctgc cagagaatgt tgattttacc ggtaccggct ctccacttac
    12841 acaatgcaaa gagtttgtta atgtgacatg tccaaaatgc ggtcaagatg caactcgaga
    12901 aacagataca tttgatacct ttgtcgaatc ttcatggtac tatgctcgct ttgcttgcaa
    12961 aggtcaggaa aatgccatgc ttgatgaccg agctaaatat tggacaccag tagaccaata
    13021 catcggtgga attgaacatg cagtgatgca tctgctatat gctcgatttt ttcataaatt
    13081 gatgcgcgat gaaggattag ttaattcgga tgaacccttt aaagctttat taactcaagg
    13141 tatggtttta aaagacgggc ataaaatgtc aaaatcatta ggtaatgtgg ttgatccaaa
    13201 ccatcttatc aatacctacg gagctgatac cgctcgctta tttgtcatgt ttgcatcccc
    13261 tcccgagcaa tcactggaat ggtcagattc tggagtcgaa ggagcacacc gttttttgaa
    13321 acgagtttgg gcgttttcac atcagcatag agacatgctc attgatatta acgatagtat
    13381 tttgagtggg aatggtcatg tcgactggaa ggaagcggag tctcgattaa aaaaatcccg
    13441 tcatattgtg catcaaatac ttgctcaggc aacccatgac tatgaccgga atcaatttaa
    13501 cacagttgtt tctggctgta tgaaattatt taatgaaata tctgattact ccattgaaac
    13561 tgaaaatgat aaatttttta tacactcaag catcagtata ttgctgaggc tactcgctcc
    13621 aattaccccg catatttgtc attgcctttg gcagcaatta ggttttgata aagcaataat
    13681 tgatgcgccg tggccaaagg tggataaaag tgctctaaaa actgatgaag tagattatgt
    13741 tgtacaggtt aatggaaagc tcagagctca atttactgca agcactgatg cgactgaaga
    13801 ggaattaatt gctgcagcta aggaacatgc ccacaatttt gtggtaaacc ataccattaa
    13861 aaaagcaatt attgtcccgc ataggcaact aatcaacttg gtcattggat aagcatgtgt
    13921 gattcgtccc ggcaatctct aaatatttta cacaatttct caagttatgg gtggccttac
    13981 ctgtaagtgc aaataagaga gggaaaagaa gaatgactca gatccacaaa tacttagcat
    14041 ctcttatact gatttggctg ctaactggtt gtggttttca tttgcgtgga gcaattgata
    14101 tccctgagtg gcttgataat gtttctatta tttccaggga tggaaataaa gaactggttt
    14161 ctatactcaa atcccagctg gatggctata aaattgatgt caattctgac ccatccctcg
    14221 cgaaatactg gttaattatt aacagttcca gtttacagca gcaaattgtt agcattggtg
    14281 caagtaccaa tccaagacaa tatcaattaa tcttaaccat tgaatttatg ttgcagactc
    14341 ccaaagggca aattattaaa acccccagaa aagttaatgt gagccgtcaa ctcaccgtga
    14401 ataatgacag gatattaggg tcaaatgaag aacaagctct tttagtcagc gaaatgaggc
    14461 gtgatgccgc tattcaaatc attaatcgtt taaaccgacc atgatatgca aataaatcaa
    14521 caagctctta ctcagctttt acaaaaaaaa atacatcatc ttaatatact aatcggacaa
    14581 gatcattatc ttattgacga agcactcaat aacataaaat caatgcttaa aaaatcgtct
    14641 gaatgtgatg agaaaatcct ctctattcag tcccctgaag aatggcatgt cgttctggaa
    14701 gaaacaaata attactctct tttttatcaa acagttattt taaccatttt ctttgataaa
    14761 aaaacgatag atacaacggg aaaaaaaatt cttagtgaat atctcaaatc aagcaactct
    14821 catagttata ttattatcag agcccctaac cttaccgcca agcaacttca atggttaaca
    14881 catgatgaac aagcgctgat tatcattgca caaccattaa gctcagaagc aatgaagaga
    14941 tggattaatg agcaattaaa attaaataat atctcttttg atccatatgt tcctgaatta
    15001 atttatcaat atactaaagg aaatatgcta gctaccgctc aggtaattga aaaaattact
    15061 ttgagcaata cacccggcac tctcattact ccttacctgg ccttggaaca tttatcagat
    15121 caatgtgatt tttccctttt tgaactgatt gattcatgcc tacttggtca tagcgataga
    15181 gcaattcata ttttaagaca agctgccaat aataaaacag aagtcacatt ggtattatgg
    15241 atacttaccc aagaaataag aacgctatta caattattat ttctaatgca acaaaagatg
    15301 gatatcaaaa tggcatctaa tcaattaaaa atttggactc aaagagttaa tctttatcaa
    15361 tccgctgtta aaagattaaa tactttactt ttacagcaac ttcatcacta ttgccaatct
    15421 attgatgata aaatcaaatc caactcaagc aatcaagcat ggaatgctct agagaacatt
    15481 tctctttcct tatgtttagg gcaattacta ggtgatgcat gcatagcata gcaatatttg
    15541 gtggaacatt tgacccggtg cataatggtc atatcaagac cagcttggca attcaagcta
    15601 actttggctt tgattcctat tattttttac cttgtaaaag ccccgcaatc aaacctccgt
    15661 cctttgccag cagtgaacaa agagtagaaa tgctaaagtt agcgctcaaa ccttaccctg
    15721 attttaaaat agatactcga gaactggatc gagatacccc ttcttatatg gtatacactt
    15781 tgcaaagttt cagacaggaa tataccgact catcattaac tctaattatt ggatacgatg
    15841 gattattaaa tctaccacaa tggtaccaat gggaaaaaat aatatcctta gccaatttac
    15901 ttgttatcaa tagagaagag ttcttccaac agcctgtgcc aaaatcagtg caaactctac
    15961 ttaaccaata tagaaatgat gataaaaata tactactaaa tcatcatgct ggtagtatct
    16021 gcttatacaa tgctggacac tatgatatat cctcaacaaa aattagagaa caattaaaac
    16081 aacataaaga tgtaaagagt aatttgcctg atttggtata cgattacatt aagaagcagg
    16141 aactgtatca ataatatttt ctgttttatc aacagtcttg acctgcttta taaattcagg
    16201 caaatggcta tatagcaaag ctacaacagg atcaaatcgt gcataaagtt tggacttttg
    16261 tgaatattcc tgataaggta aagaggtcat tttaacagca accataatca gggcaacaat
    16321 aaaaacgcca cgtaaaaagc caaaacccat tcctaaagtt cgatcagttc cgcttaaacc
    16381 agctcgtttt aagataaagc tgagtattgc atttactacc cccccaacaa aaagggtggc
    16441 aaaaagaata ataataaatc caatagccgt tcgtactgac tgttcctcga tataagaact
    16501 taaccaggga tccaggcttt gtgaataatt aaaacctagc caaatagcta atatccacac
    16561 acataatgca acaagttcct ttacaaaacc acgaaacaat ccggtcaata cggataaagc
    16621 gataattgca acaattgcta tatcaaccca ttgtagttgc ataactaact aactccagta
    16681 ttcacaacaa aaccatttaa ttgcatggca ctagctaatt gagtttttaa ttttattaca
    16741 tcttttttaa cgggcgaatg gccaacaact actttataaa ctactccatt tctgctattg
    16801 gacttaagat aattcgcctt gtagcctttg ctgcgcaatc gattaaccaa agcttgagca
    16861 ttagctattt gtgtaaatga agccagctga acagcataaa tatcttgttt aactgctggt
    16921 tttctcgcag caacaatttg gctgaccttt ttgttagtat taacctttgc cgtaacaact
    16981 ggagatcttt tcaccggtgg ttttgctgca gccagggtca ttactttctt tgctgtcgca
    17041 ttcgccgctt tatttactgc caatttaatt ggctctgatt taattgagtt ctctctttct
    17101 gcttcaagtg tggcagcttt agatgttctg atgagattgt cttcttctgt gctgatgggt
    17161 tctgcttttg ccaggttaat tgattttttt tcaactggta ttgggggtat ttctgtctta
    17221 gccactttta tagttttaaa tacctctttt tcatcagtaa tcactacgtc aggaggtaat
    17281 ggttttggtg gtaatttcac attgacacta aagttacttt cagggctttg gctggatttt
    17341 tttattagtg caggagcaaa aattgccccc aatgaaataa taacggccaa acctatcaag
    17401 cgatgtttaa ctttctcatc cattatcagt ttcattgatt ctccttctgc tccaaaagat
    17461 tatgttgggc agccattaca tgactaaccg taaaaaatga accataaaca ataattaaat
    17521 cgtcatctga tgcttgcttt aatgccattt caaaagctgt aaatgggtca ttataacaaa
    17581 catctagaaa aatttctgct tccctgaata cagaaagcaa ttcctcttga cttgcagcgc
    17641 gcttattatg taattgtgca ggataccatc gattaacaca atcccttaaa ggcataatca
    17701 atccataaag atctttatcc tttaaagcgg agaaaacagc atgaatttgc ccttttacat
    17761 tgattttttt tactgtatca gcaagtaatc gaactgactg cggattatga gatacatcgt
    17821 acaaaatatc agtctttcct ttttgtaatt gcaaccgccc cgggatataa atgagctcca
    17881 tcgctttctt tagattctta taggatacag gcaaatcatt ttgcatcaaa tgacaagcca
    17941 ttacagctgc tgccgcagaa cgaagttgaa tttttggcct ggtcaattgc tcaaatggct
    18001 taccttgaca ggtaaccccc caatggtcag cttcctcctg ataaaaatag tctttaccgt
    18061 ataaataaga tgatgctcca agttgtaatg caacctcaac aacacttttt ggtggtgaga
    18121 gatccgcgta gataaaaggt ttgttttttc gtagtattcc ggctttctca taccctatca
    18181 actcaagtgt gtcaccaaga aaattctgat gatcaaagtc gatagtagta atgatcgcta
    18241 aatcagtatc aacaatgttt gtagcatcca accgtcctcc catgcctact tcaagaatta
    18301 tcagatccag atcatgctgt ttgaaatacc ataaggcagc aagtgttgcc atttcaaaat
    18361 aggttaaatt ggtagggtaa cgtgcttctt caatcacaga aaaagcctta cataaatctt
    18421 tatcttgaat cggtattaaa ttaattcgaa tacgttcatt aaacacaaga agatggggcg
    18481 acgtataggt accaactcta aaacctgact ggtgatagat tgtttcaagt gctgtgactg
    18541 tagaaccttt accgtttgtt cctgcaactg ttattacctt gcaatttggt cgctgcaaat
    18601 ttaatttctg cgctacagtc aaaactctgg tcaaaccaag ctgtatttcc tgggtatttc
    18661 tggtttccaa atgagtcaac cagtgattta aatcccattc actccatggc gtgtgcaaaa
    18721 aaactctccg ctcctttatg agtcaatttg gaaaccaatt cggatatagt tgctcgaaga
    18781 tgtttacgtt caactatcat atcaatatgc ccatgttcca gtaaaaactc acttcgttga
    18841 aagccttctg gcaaagtttg tctaaccgtt tgctcaatca ctcttggccc agcaaaacca
    18901 attaaagcat tgggttcagc aatgattata tcaccaagac tgccaaaact agctgatact
    18961 ccacccatcg ttggatcggt taacacaaca ataaaaggca acccagtttc agcaaatttt
    19021 gctaacgctg ctgatgtttt agccatttgc attaaagaaa ataatccctc ctgcatacga
    19081 gcaccgccgc tggcagtaaa acaaatgtat ggccgctttt ctgttgcagc aacattcact
    19141 gcccttacaa atttttctcc aaccgttgct cccatggagc cgcccataaa attaaactca
    19201 aaggcactga caaccaccgg ttgttgcatt aaaaaccctt taacaacaat taatgcctct
    19261 ttctctcctg tcgctttttg cgcttgagaa attctatcct tgtatttttt ggagtccctg
    19321 aattttaatc tgtcctgtgg ctccagccca gcggccactt cttcttgccc tgactcatct
    19381 agaaattgag caattctcaa gcgcgcggaa atgcgatgat gaaaattaca agatgggcaa
    19441 accataaaat tttttgccag ttcggtactg taaagcacct cattacaacc agaacattta
    19501 acccataaac cttccggaac ccccttcttt tgagaggttt ctgtccttat tcttgatggt
    19561 aacagttttt taagccagct cataaattag aacccagata ttatcaaatc ggattattat
    19621 acacagaaaa aaataatctg acatgtctat attggacttg aatttcacat aaaggaataa
    19681 ggtattttac caaatcgact tgataaccag aaccaaatca cactgaaaac caaattactc
    19741 tttaaaaata ttcaagtcca tctgaggaat ttcagacgcg ttgattatat tgcaatcgat
    19801 ctttctattt atttaacacc ctaattttat tcaataattt tttgcgactt ttcaaaaaag
    19861 cactttccga actaaagttt ttttcactat ttccgataac taaatcgggt acagtaaatt
    19921 cacaacaata aatcgaattt actgaattag aaatgtccca taccagggat tcaggtctga
    19981 ggagactaaa actatggctc aagtaatcaa cactaatgtg gcttcgctta cagcccaacg
    20041 caatttgggt gtttcgggca acatgatgca gacatcaatc cagcgtttat catcaggatt
    20101 aagaattaat agtgctaaag atgatgcagc aggactcgca atttctcaac gaatgactgc
    20161 acaaattcgc gggatgaatc aagcagttag aaatgctaac gatggtattt ctctggctca
    20221 ggttgctgaa ggagcgatgc aggaaacaac caatattttg cagcgcatgc gtgagctttc
    20281 cgttcaagct gctaactcaa ccaataactc atctgaccgg gcttcaattc aaagcgaaat
    20341 ttcacaatta aaaagtgaat tggagcgtat tgctcaaaat actgaattca atggtcaaag
    20401 aattctcgat ggctctttct ctggcgcaag cttccaggtt ggggcgaact caaaccaaac
    20461 catcaatttc agtatcggta gcataaaagc ttcttctatt ggtggtattg ccacggcaac
    20521 aggaacagaa gtagctggtg cagcagcgac agatattact atcgcaattg gaggaggagc
    20581 agcaaccagt attaactctt ctgccaattt taccggggca ctaaacggac aagatgcttc
    20641 gtctgcctat gccaaagcag cggctatcaa cgatgccggg ataggaggac tatcggttac
    20701 agcctctacg agcggtacac aaacagttgg cgcaataggt ggaaccgcag gtgataccta
    20761 caacttaacg attaatggtg ttgcaatata tactaacctt aacgtagcaa cggcattaac
    20821 caactccgat cttcgggatg ccatcaatgg tgtcagtaac caaactggag ttgtcgcgtc
    20881 acttaacgga ggtaacatga cactcactgc tgctgatggc agaaacatta cggtaacaga
    20941 aagcggtaca ggttttactg caggtactga cggtttaact gtgactggtg gcgcatttga
    21001 tggcgcgcta cgcggtactt tatcaatcag cgctgttgat actatcgcga ttggtggtac
    21061 tgttgctaac attggtttaa gtgccaatat ctctaaagac acagtaggga tagattcact
    21121 ggatgtgagc acagcatctg gcgctcaaac agcgattaag agaattgatg cagcactcaa
    21181 tagcgtaaac tctaatcggg ctaatatggg tgctctgcaa aatcgctttg aatcaacgat
    21241 agccaacctg caaaatgtat cagacaactt atcagctgct agaagtcgta ttcaagatgc
    21301 tgattacgct gctgaaatgg ctagtttgac caaaaaccaa attctgcaac aagcaggtac
    21361 agcgatgttg gcacaagcta atagcctacc gcaatctgta ttatcgttgt taggtcgata
    21421 ggatgtcaca atcgaagtgc agcaacaact gcacttcgat ttctttatac ctcggttgat
    21481 gggtataaaa aggagtaaga aaaatgagta ttgaacctgt accctcagtc aatagggcag
    21541 ctcaaacaga tcaaataaaa atagttgagg aagcctctaa gccagaaatt aaaaatatgg
    21601 tttatgattc cgctttggat gccgaaacaa aacaggcgct ggataaagca acaggtttgc
    21661 ttcaaactat aataacagag aaaatatccg ataaagtatt acgtaaaatg ccttctgatg
    21721 aatatttgca attactaagc ttgcttgatg atattattag tggttctata gacaagcacg
    21781 tttaataatt tgaaatattt attaaacatt tgactagaac aataacaaca tagaaaaggg
    21841 atactctatg gaactctctt caccaggcgt agggtcagga cttgatgtaa aaaacctggt
    21901 cgatgcgata gtcacggcag aaataactcc atcgcaagtg cgtcatgata aacagttaaa
    21961 tagtgtcaat acagagttgt cggcgcttgg tcaattaaaa agctatttaa ctaacttgca
    22021 aacttcgtta gccaagctct ccagtcttag ccaattctac actatgaaat caaccattag
    22081 tgatccgaat tacttttccg ctactctggg gactggcgcg actaaaaata cttatcaaat
    22141 tgaaatacaa aaactggcac aacaacacag tctggcaagc tcttacatga ctaatactgg
    22201 aagcggaaca ataaccatag attttggaac ctataacagt gataaaacca cttttacacc
    22261 gaatccttcc gctacgtcag tcaacataac cattacgcct ggcaacgata gccttgttgc
    22321 ggtacgagat gccattaata atactaattc aggaataaca gccagcatag ttcaagatag
    22381 ccagggctcc aggctaacta tcacttcgtc aaaaaccggc gaaaattatg ccatgaaaat
    22441 tagtggtgga gttgcctcat tgaattatga tcctaccatg ggagtgaatt cattaactga
    22501 aacagtagcg gcacaaaaca gtactgttaa aattaacgga ttaactttgg ttcaaagttc
    22561 taatcaattg aatgaggcaa taactggcgt caccattgat ctaaaaaaag cagagcttgg
    22621 gaaagttgtt accttaactg ttgatgataa taaagatcag ctcacaggct taatcaacga
    22681 attcataaaa caatacaatg acaccatgac tttcttaacc aacttaactg gttataacaa
    22741 tgaaaccaaa caaggaggag tatttcaggg cgatccgcaa tttcgcaatt taaaactcaa
    22801 tttaaacaag tgggcaacta cccctttagc taaccaggaa ggaccaatcc gctcattagc
    22861 cgatttggga ataatcacca ataaacaagg tttactggaa atcaaacaag acaagtttaa
    22921 aactgcactc gataatcact ataaagaaat aggagcatta ttcgcaaaga ccgcgaaggt
    22981 taatgataca ggcattaaca tcaagtccat caattcaact gtaaaagcgg gtcattatga
    23041 tattgtattg actgaattta ctcctggagt cagtatgtct ggaacaatag gaggtttatc
    23101 tgctacctca tctaatggca ttacattgac aggaagtggt gatttaagtg gtctgtcgat
    23161 taatgtattt tcagggtctg ccggagcccg tgggcaaata actgttacag atggtattgc
    23221 agtccaatta agttccttgc tagatactta cattgaaatt aaaggcgatt tggaccaaag
    23281 aaaagaccaa ctcaataatc aagccaagca attgaacaaa atacaaaacg aaattgatat
    23341 cagacgtaaa tcaattgaaa ctcgctattt aaaacaattc accgctctgg acactctact
    23401 cagtcaatta caaagcacta gtgtttttct atcccagcaa ttagccaatt taccccaatt
    23461 aaaattgaaa taataggagg taatgagaat gaaaaaccta tattcacaaa tttctagcca
    23521 atacaaagcc atagagctac aaactcgcat agaaacggcc tcccctcatg aacttataga
    23581 tttattactt caaggagcca gatctcacat tgctactgct cagggtaata tacagcgtaa
    23641 ccaaattaaa gaaaaaggag aacacattgg caaagctatt agcatcattg aaggtttaaa
    23701 aatgagtctc aatcatgata aaggtggcga aattgccgaa aatttactcc agttatatga
    23761 ttatgttcaa caaatactac ttaaagccaa tttaaaaaat gatgaagatt tattagctca
    23821 gtccaatatg ctattagctg aagtgcatca agcctggcaa gcgattaact ctaatgctgg
    23881 taatgaactt taatatttgc aatgagctcc catgattcag catttgcttt ttacggcttg
    23941 ataacaacca aaattagaat cttcttggct tggcaattat taaacccagc ccatctattt
    24001 ttattctccc aatgcaaata gtttaaggct tccaaatatc aataagatat gaggaattgt
    24061 ctggaagtct tgtagttatc gaaatttttg tacattttgg taatcaagaa taggattata
    24121 attttggcaa atttgtatag atttatcgaa ttggaatata aagtttttcc attatatgcc
    24181 gccaaaacag ttaggatgaa taaattaatt tcattatgaa tacatttaaa tcatcactgg
    24241 catctgtaat ttgtctctta cttagcatgc aatcggcaca ggcgcaaatg cgatatggag
    24301 aaactctctg caacttgcct gaatattttt gcatgaaaat aaaaagcggg gacagttggg
    24361 ataggctttt ccctaacatc gaagagcaag atattgtcag acgcattaac cgaatgaata
    24421 tcaggctcaa acccgggatg attattgctg ttcccaaaaa tatagagcgt ttaaccatct
    24481 atgacgtctc gccttttcca agatatattg aaccaagtgg tgaaaaaaca atctatgtca
    24541 gtcaaaaaaa attggcatgg ggagcttatg atgaagacgg ggaattgctt tggtggggac
    24601 caatatcatc aggtgttgga aaatgtaaag taataggtgg ttgttctaca ccaacaggtt
    24661 catttcgcat tatacgcaag caagatattg attgtgtttc aactgttttc ccaagaaggg
    24721 caaatggcga acatggtggt gcactgatgc cttattgcat gcactttttt agaggttatg
    24781 ctttgcatgg aagttatgaa gttccaggat atagggccag ccacggctgt gttcgcatgt
    24841 ttattgaaga tgcgcgttgg ttaaatgagg aatttatcga cctaccaggt ggaggaatga
    24901 gaggaacgcg tgtaatcctt gatgcgccag atgctcaaga gaatctcgat aacctacaat
    24961 aatatattga aaacacaatc aaaaaactca cccccaatag acaaatagtg gtcaacctga
    25021 taatatttta tttagtgaat tttgccatga ggtgtgttaa cgtataaatt cactgtgtga
    25081 ataaaataag tacattttta atcaaagatt agaatatatg gaaataccat ctatatcctc
    25141 ttttttgact aataactttc atagaatcta atgattggat aaatttggag aatttttaaa
    25201 taatggctca ttacaatgta caatccgaat cgctcaataa agcaagtatc agcttaatta
    25261 tgccctggat ggtgtggggc ttgggttgct tattctattt ttatgaatgc ttattgcaag
    25321 tatccccaag cgtaatgagt aatgaactca tgagagattt ctcggtaaca agtcatactt
    25381 tagggattct atcgggcatt tatttctatt cttatgccgc catgcagctt cctggtggtg
    25441 ttctaatgga ttattttggc ccacaacgtt tattaactct tgccacagtg gtttgcgcct
    25501 tgagcactat agcctttggc atgacagata atttcttcat ggcctgtgtc gcaaggctaa
    25561 tgattggatt tggttcagca tttgctgctg ttggcaccat gaaattggca gccaactggt
    25621 tcccagcaca aaaattcgca ttattgactg gcttgatggt taccataggc atgatgggtg
    25681 caataggtgg agaaacacct ttggctcttc taattgataa ttttggctgg cgagaaagca
    25741 tgattataat gggtagtgtt ggccttcttt tggcaacttt gctcatcttg attgccagag
    25801 atactcccaa gaattttact cctgctatcc atcaacatcc tgtcgaagaa gaacgtttaa
    25861 tcccaagttt attcacattg ataaaaaata aacaactttg gttggttgct acctatggcg
    25921 ggttaatgta catggcaaca cccgtatttt gtgggctttg gggggttcct tttctaatga
    25981 ataaaatgct aatagcaaaa tctacagcag ctaattttat ctcattggta tttgtgggtt
    26041 gggcaatagc cagtccttta tggggtctgt tttcaaaccg tattggatta cgtaagccgc
    26101 ctatgtatat tggatgtgta ggggcaatga tttgttcttt actctttatt tttgctccca
    26161 tcacctcttc aattactatg gaaatcctgc tgtttgcatt tggtatcttt tccgcaggct
    26221 ttttgccagc tttctcagta gccaaagaat tatgtaataa aaaatatgtg gcaacagggc
    26281 ttagcttcat gaatatgatg aatatggttg gaatagcgtt agcacaacct ttaattggtt
    26341 atattctgga taaaatgtgg caaggtcaaa tgatggggac tgtgagagtt tatccattgg
    26401 aagcttacta cactgctttg tcaatccttc caataggcat gttagttgcg ttactaatat
    26461 taccgaaaat caaagaaacc tattgccaaa gtgtcaatta atgaaataat accgcaattt
    26521 ttcaatttgt agaatcaaaa tggttaaaaa attatatatt aaaacaaatg gctgccaaat
    26581 gaatgaatat gattcttcta aaatggcaga agtactttac gcttcccatg gtttggtcaa
    26641 aactgatcaa gtcgaggatg ccgatgttat tttattaaac acatgctcaa ttcgagaaaa
    26701 agcacaggaa aaagtttttt ctcaactggg tcaatggcgg gaatataaag ctaaaaaccc
    26761 tcatgtgctc attggagtgg gaggatgcgt agcgagccag gaaggctcag acatcataaa
    26821 aagagcaccg tttgttgata tcgtatttgg gccacaaaca ttgcacaggc tccctgccct
    26881 gctcaatgaa aggcttgaaa aaaataaatc cgttgttgat atcagctttc cagaaataga
    26941 gaaatttgat catttacccg caccaagagc agaaggccct acagcttttg tatctataat
    27001 ggaaggctgt agtaaatatt gcagcttctg tgtagtgccc tacacgcgtg gcgaagaaat
    27061 cagcagacca tttgatgatg tattggctga atgttatcaa ttagctagtc aaggcgtcag
    27121 agagattaat ttactaggcc aaaatgtgaa cgattataga ggtattatgg ataatgggga
    27181 tatagccgat cttgccctgt taattcatta tattgccgct atcgatggaa taggaagaat
    27241 acgtttcaca acatcacatc ccttggcatt ttctgaaaat ttaattaatg cctacgccga
    27301 ggttccagag cttgccaatc atttacacct tccggtacaa agcggatccg atcgcatttt
    27361 atcgttaatg aaacgagggt acactgcttt ggaatttaaa tccaaaattc gtaaattaag
    27421 gaaaattcga ccagacattc gcttatctac tgacattatt gtaggctttc ccggagagac
    27481 agacaaagat ttccaggaca ctatggatct tgtgcatgaa atcggttttg atacctcttt
    27541 cagttttata tacagcccaa gaccaggaac tcctgcagca aatttacctg atgacacgcc
    27601 tatggagctc aaaaagcaac gtttacagat tttacaaaac agattattaa tgaatgcagc
    27661 tcgttacagt gaatcaatga taggcagcaa acaaaaaatt ctggttaccg gattttccaa
    27721 gaaaagttcg cagcaattat ctggtcgtac tgaatgtaac cgtgtagtta actttgatgg
    27781 accacctcat ttaattggac aatttataga tgttcaaatt agtgacgcac tacctaattc
    27841 attgcgagga aggctgcttg aaaaggagat gcagccagcc taatgattga gcatatccag
    27901 caacaattat cagtacctcg cgaattacat aaccaacgag tagatagcgt gcttgccagg
    27961 ctgctccctg agtattcacg ctctcttatc tgtaactgga ttaaaacagg tgctgttacc
    28021 cttaataatc tgccttgcaa acccaaagat aaagcgctgg gcggtgattt aattacgatt
    28081 aatgttgacc atacaatagt taatacagat tttaacttgt gtaaacctga gaacattcca
    28141 attcagataa tctatgaaga cagtgaagta attgttgtta ataagccagc cggtctagtt
    28201 gtacatccgg gtgcgggaaa caaggaacac acactagtta atgcgctgct tcatcattgt
    28261 cccgagttgc aactacttcc tcgtgctgga attattcaca gattggataa agataccact
    28321 ggtctgctga tagtggctaa aaacctgatt agccatactt ctctcatcag gaaaatgcaa
    28381 aatcgcgaaa ttaagcgcca ctacctgaca ttagttcatg gttatgtcat ttctggtgga
    28441 acaatagata cgggttttgg caggcatccc aaaaatagac taaagatggc tgtaactgac
    28501 ttcgggcgtc aagctgttac ccattataat atcaataagc attataaaga ctttacctta
    28561 ctgaatgtta gcttaatgac aggacggact catcagataa gagtgcatat gaactatatt
    28621 aaccacccgg ttgtaggaga tcaactgtat ggaggaagaa taaagtttcc tcctaatgct
    28681 gatgaggcaa tactctcagt tttaaaacaa tttaaaagac aagcacttca tgcctgttcc
    28741 atttcatttt gtcatcctgt aaaaaataca gaaataacac tgaatgcatt attacccgat
    28801 gatttcgaac tcttgttaaa cactctggat caacacgatg aaaaatagat ttgccaattg
    28861 gccggctcct gaaaatatta ccgctttgag tactacacgt ttgtatggtt tcagtgacgg
    28921 accctatgcg agcaacaacc ttgggttgca tgttggtgat aaccaacagc atgtactcaa
    28981 aaacagacaa cagctcattg aacttattga tctgcctgca gaaccaatct ggctagagca
    29041 aacacatagc acaaaatgca tcacggtaga aactgaaaaa accagagatg ctgatgctgc
    29101 aattactcgt ttacctaaac tccctttagt cattatgaca gcagattgcc tacccatatt
    29161 gctatgcaat cgacagggaa ctgaaattgc cgccattcat gctggatgga aagggctgta
    29221 taatggtatt attgaaaata cactaaagaa gctaaatagc aataggacag atataattgc
    29281 ctggataggc cctgccatct gtaaaaaatg ttatgaagtg ggtgaagaag tttatctgtc
    29341 ttttaccaat aaatactcct taaccaggaa tgcgtttgag cctgttcata gcaaatggtt
    29401 agctgattta ccccaaattg cagaaatagt acttaatttg gaaggggtaa aatcagtatt
    29461 tcagtcaaat ttgtgtactt ttgagttaaa aaatgaattc tattcctatc gcagagaatc
    29521 acaaacaggt agaataggta ctttcatctg gattaatgag taaaatcagg attaataata
    29581 tgattacacg tataaaatta ttcatagcaa gtctgctggt atttttcatc cctgtagccg
    29641 atgcagcaca agatcttaca aacatgccca gcctggctcc tgttctgaaa aatgctatgc
    29701 cggctattgt taacgtggct gttcaagggt acttgcctaa taatgtggca tccggaaatg
    29761 cagacgatga tgacggggaa aacagtaagc agccttccag aatacctgaa aaaggcagaa
    29821 aatttgaaag tatagggtct ggagttatca ttgaccctaa aaatggcatt attataacga
    29881 atgaccatgt tattcgtaat gccaatttaa ttactgtaac tcttcaagat ggcaggagat
    29941 taaaagctcg cctgattgga ggtgatagcg aaacggactt ggctgtttta aaaattgatg
    30001 ctaaaaattt gaaatccctg gtaattggtg attccgataa gctggaagtc ggagattatg
    30061 ttgttgctat tggaaacccc tttggattaa acagctttgg aaatagtcaa tctgctacgt
    30121 ttggaatagt gagtgcctta aaacgcagcg atttaaatat tgaaggtgtt gaaaacttta
    30181 ttcaaactga cgcagctatc aatcctggta attcaggagg tgctttggtc aatgcaaaag
    30241 gcgaattaat tggcattaat acagccatta tttcacccta tggtggaaac gtaggtatcg
    30301 gctttgcgat cccaattaat atggcaaaag atgtagcgca acaaatcatt aaatttggct
    30361 ctattcatcg cggtttaatg ggtatatttg ttcaacattt aacaccagaa cttgcccaat
    30421 caatgggata tgccgaagat tttcaaggag ctttagtatc acaggtcaat caaaattctc
    30481 ctgctcaatt ggctggtcta aaatcgggcg atatcattgt acaaattaat gataccaaaa
    30541 taactcaggc aacacaggta aaaacaacca ttagtctgtt gcgagccggt tctactgcta
    30601 aaattaaaat attgcgagat aataagccgc ttacattaga tgtagaagtc acagatatca
    30661 aaaaacatga acaaaaatta caatccaata atccatttct ctacggatta gccttacgta
    30721 attttgaaca agaatcacca cctcatggta atgttgttgg agttcaggtt gtaggtgctt
    30781 cggaaaccag tgcgggctgg cgagctggct taagaccagg agatattatt atttctgcta
    30841 ataaaacacc ggttaaagac attaaatctt tacaagctgt tgcacacgag aaaaagaaac
    30901 agctattagt ccaggtgctg agaggagcag gagcactcta cctgttgatt atttaaattt
    30961 tttatccaaa taatgaagta aagcatcctc tttatttaag cccatctctg agtgatgggc
    31021 ttaaattttg ataaattatc ctgcttttgg taatgctgac tatcaactat tccccctata
    31081 ctccaaaaca atcccttgct tggggaaata tcacttttca atatatcaac aaacttcttt
    31141 ttaaatacgc tattctgctt tgaaaaaata atgcaaattc aatttaatat aaattaatta
    31201 taaaaaactc aggaaacagt cttgacataa acacatgaaa aagacaaaat cacgacctct
    31261 tgtggctatg tagctcagcc ggttagagcg cggcactcat aatgctgagg tcggtggttc
    31321 gagtccaccc atagccacca tttacatcat atgccccctg attgagaaca cttgaagtaa
    31381 gcaatcaaac cctactcccg atttttgtct ctcattttgt cacttcaagc aatagaatat
    31441 ctactataat caaatcaggc cctgcccaga aataatattt gagatcatct tgcatcacga
    31501 aaagaaatta tagtagcatc gttgcttatc acatttcata atacggattt tattttgttc
    31561 aggattaact taatacgata tgtggcactt ctgtttatta tcacattgaa tttagattgt
    31621 attagcgttg cattttcatc tcaggtaatt tatggaacac aaaatgctgc acgagttact
    31681 gggaaaagtc tgaaaaaaat ttattttctt cagttaggcg cttttaccca aaaaaacaat
    31741 gctcagcaat tggcaagaaa aattcaagcg agaacacaat accctgtaat aattagtcat
    31801 tcaggcaatg ctttctcggt tcgtgtaggg cctgtcaata cgcaagaact acatcaactc
    31861 agcacaaaac tttcacaaaa ttccaatatt actccaataa aacataattc atcgccaaag
    31921 caaataccga ctattcagtc aaaactgcca atacttgtta aggaaacttc tcgccctctt
    31981 ccaactcatt ctgtgactaa agaaacaatt gatgggaaag gaagctggta tgtagctgct
    32041 aaaattggag cacaagaaac aacaccagat gatcagttta cagtaaataa cgacagtggc
    32101 tttcccagcc cttttgataa agatatttat accaacagtt ccgattcctc cctgctatta
    32161 ggcattggtg ggggatatcg atttacaaat ataattctcc ccgcaatctc tgttggagta
    32221 tactattccc atttctttaa tatggacatt aatggacaag tgatccagta cagcttgccc
    32281 gaattcacca attacaatta tcaatggaaa gtaaaatctg atttggtttt agcttctgta
    32341 aaagccaatg tctttcgctg gaaaaattta atgccatttg tacaaggagg tgttggagcg
    32401 agctcgaatc gagcaagtca atattatgag aatgccctgc ctgatgtcac acctcgtatc
    32461 agtcctaatt ttgccaatca tacgaccact gtgtttgctt accagctggg cgctggcctt
    32521 gattgggaga taagaccaca atggttgtta tctttagggt atgaatattc tgatttaggc
    32581 aaagtacgtt caggttatgg tactgatagc tggaatagag agacattgtc ctcgaaaaat
    32641 ttaacaagca atgctggaac ctttggcttt acctatttat ttcatggata accaaaagtg
    32701 aggcacgggc taaaattcca aaccttgaga aaccacaaat aatttgtggc ttcccaacgg
    32761 taacttatca aatagaactg atttaatctt cataaaaata atgggtgaaa ggaccaacat
    32821 gaaatactca actcgcatca taatcaccac aatccttctg ttatttgcct tgctgggtta
    32881 tgctggaaaa gatcctatca gctggcgagt cagtggagta tttcctgctc aaagcagcgt
    32941 tggtcagact tatactgtga cttatacttt taccaatcaa ttgccttttc gattggccaa
    33001 ggccttgatt atagaacgat tggcaagccc caacacggaa ttcaatttta atgatcaatg
    33061 ttccggtcgg ttattaaatt ccggagaatc ctgtacggta gttgtggcgt taaaccccct
    33121 tgtaattggt cttaaaacaa ttcaattgac tattgcagga tacagcaagg atagagtccc
    33181 tttacctcaa gcaagcacct taagcacagg gcagtccttg agtccaatcg ttagctttgt
    33241 catggatggc tttccttctc aattagcgac aggctcagtt tccagttata aatttacgtt
    33301 taccaataac ggggctattt cagctaccaa tttatcagtt gatgtctcgc aatcattagg
    33361 ggcaccgaat tttactacga cgtgcaataa cacattgact cccaacggag gggtttgtga
    33421 agtcgatgga acgtacacag taacttccgg gaatccgtcg attcaacaag tcactgccag
    33481 tctgtcttat acaggcccat cgggaagccc ggtaacagca cagacattca cgcaagtaat
    33541 caatccagca gctcctcttg taggcacttt agtaccgcca aattatctac ctccattgat
    33601 gattgctggc aaccagtata cagcccagtt tttattcacc gccagtggaa ctgtggatat
    33661 tacagctcct ggctcaatag cctgtgacca gggaggtaat gattgttcgg caagcttaag
    33721 ctcttttaac tccagctgca ccctgcaaac attgaccaat gcagcctgcc aaatgaccgt
    33781 cactttcaca gcacctgctg ctaataatcc cccacggact tatgttctga ccgcaacagt
    33841 tccatactct gttggaggaa acccccagcc tgctgcaact gccatcacag cgggtacagt
    33901 cctcgccaca ttgccaacag aaagaacagt cactttagtc aatgagtgcg attttgatgt
    33961 gtggttttcg ctgaatggaa gtcaattagg tagttcacct aactgcccag gcacaccatg
    34021 tccaaatggc acaagctgta ataccagtac acacaaatgc ttttggagca atcctattcc
    34081 aaatatacca gttgcccctc aggcctattt attgacggct ggtggtggca caaataatgt
    34141 cactattcct gttaccaatg ctgatcctaa tattcaatgg agtgggaata tttcagcaag
    34201 cacactgtgc aatggtgtaa cctgtcaaca agcagcttgt gacaatgcgg gtggtaccgc
    34261 tccttgcaat ccaggtattg gttttacaca acccgctacc caggctgaaa tcacgatgaa
    34321 tctaaccacc tctgacagtt atgatgtgga agtgattaat ggttttcata tcccaatttc
    34381 tatgcaaccg atttattatc aaggagtcac tactattcct gctactccta ataattacaa
    34441 ctgcggagaa cccggtaaag acacaaccgc caatggattt ggagcttgcg attggagtac
    34501 agccactgtg ccagtcattg atcaagtgcc agggaatgga ttttactggg taactggtgg
    34561 ggggcaaggt tgttccatta cctctgcaaa tccgggttgc cctgcgatga cattatgcgg
    34621 attagacagt aattttaatc aggtttgtgg caacttttta ggatattggt cacccgatca
    34681 agtctgcggt tcaagtaatg tcccagctgc cgtacagagt tattttaaat gcaatcaacc
    34741 cctccctact tctacaacgc ctttttatcc ttccggcgct gtcttatcca acttgatgct
    34801 atgctctgtt ccaacaggat ttaccggccc aaggtataac acctgttata atgcttatcc
    34861 ttcttcttcc cccacagaca ttgcccaatg ttgtgggtgt gtagattggt ggaatactgc
    34921 tcaaaccaac aatgtagcga ttggagcaaa tccaaatacc gaaagttgta ctcagcctgg
    34981 cgccttgcaa ccacaaacca atgcccaatg gaattcattt gtacaaccaa tgatacaatg
    35041 gatgaaacga gcttgccctt ctgcttatat ttatcctttt gacgacaaaa ccagtggatt
    35101 tacttgtact aacaatctgt cagggcaacc taactccaca agctatatca tcaggttctg
    35161 tccaggaaac atcacagggc ttccagcagg agtaaatgag gggcgtgggt aaaatacatg
    35221 ctggtattaa cgcttcgtac tttatagttg caagaagcga ttcgttaata cctgtaaaca
    35281 tgaacaacaa ttactttgat ataatttccc gcacttcaaa gatgcgtctg tcagcagaaa
    35341 atttagcttg aaacaaatga ataccttcct cacgcatctt aggtgccagc tcagacaagt
    35401 accttgacaa attatctcta ttgtccagct ggtattgaac agttaatttt tccttaccag
    35461 actttgtttc tttttcctgc tttagaatgc aggcctgcat aaatcctgga aattggatta
    35521 tttccgcaac atgttttttg agccatgctt gaaattgtac aaatatctcc ggatcaatcg
    35581 ttaggtttac ttcataaatc accataattc atcttcccaa attcccatga tttgaatcca
    35641 ccaattgcac acaacaactg aatttatttt tagaataatg agtcatgcca tgattggata
    35701 cgatctacca tttggctata aacaaataat aaattgtatt ttaattcata ctttagttac
    35761 ttgaatttta aatgaccgaa tatctgtttg ccgctgattt acaatagtac ccataaagca
    35821 tactcaacat accttaaaca agtcaaagaa tgtagactat ttggcaagac atttaaaaat
    35881 caaataacaa tatgaataac gcaatattcc caccaaatct ttattaaaaa aaagttccat
    35941 attattttat tttaaatgat aatgattatc atttttattt aaaataaaac caaatgctgt
    36001 gtataagaaa ttacattagg acaagaatta ttttaggaat attaatgttg aatgttagcg
    36061 tgttagcttt cgcagacgct ggagactctt tatttaaaaa tgaaaaattg acctacgatg
    36121 gacatctgtt ttttaatgcc accaattcag caatacaagg tgaaaaatat ttactgaatc
    36181 agcagctgtc caatattaaa atgggatctg aattacaagt gactgattgg aataaaatga
    36241 aaggcttgct aatttataat accttgccta ccccagtggc tcctcaattt tattttgagc
    36301 aattttatga cgaactccaa atagagtcct caaatatttt ctttgcattt ggaaaaaaat
    36361 ggctcacatt tggaaattat aaaagtgatt taatttataa accacttaca aaagccttgg
    36421 gacaaaccaa cgaagtgacc gctattattg gatacgattc cttgtattat gctaacgctt
    36481 ctttttttaa accctattct cgcataagta catcatcact tccgctttat tacaatctga
    36541 atacaggagt acataatcaa tcaatggatg caggagtcag ttatttatac tctctggcag
    36601 aaagtcaatt atttcaatac aacaaaggtt ttggcgggct tttatcacaa tcattaaaaa
    36661 caagggttcc aggatttgca acctatttta atttaaaata taaaaaaaca agcacttatc
    36721 ttacctatgt tactgcaatc accccctttg cattagaaga catgagttat aacaatcgaa
    36781 gagcttcgcc aaaggcattg agtatacaaa atagttatga tgttaatatt aaaaattttt
    36841 cttttaaaat cattggtttt tatgactaca cttttcaagc tctggcactc ggaataccga
    36901 aacaaagact gggactggga ttgtccgcaa ccccttttcc ttatcttggt attcaatttc
    36961 agtattccag agactatagt tatggaaata atgccatagc ctcgggtatc aatcattttg
    37021 taaatggccg taatacaaaa acgaataatt tggcattaca aaccatactg aatttttaaa
    37081 ttcaggtact caatcaaaaa taaaaaacta tctctctatt atttaagcaa ataaatcgca
    37141 atacattatt gcaaatgata atcattatca tttatagtgt tgtcgtttct tacttattca
    37201 cggaaataat catggcagca gttttaaata attcttataa aataaataac tcattacaca
    37261 gtttattact tgaaaaagat cttcttgttt cacaatcaga aatggaacat ttcatatcca
    37321 tcgcgcacca gcaatgttta gatcgattgc aaaatgcagc acttcgtgaa ggattaatag
    37381 acaaggaaat aaacagcctt catatacatg attatcttga tttactttat catcaacaga
    37441 aaaagcaaaa accggaaaaa acatttgatc gatggcctct tctaaaaaaa gaactcgatg
    37501 aaagcatcaa caatcaggcg ttagccctcg cttattatca ttgctggcaa caatcgctga
    37561 agcaacaggc gaaaaaccac tccagtttat ggtcatggtt atcagagcaa cataataaac
    37621 aacaaattct gactttttta gagcaatggg gttgcatagg gcacccgtat catcctaatt
    37681 tccgcgcaaa aatggggttt attcctgaag aagtgattcg atactcccct gaatttaacg
    37741 cgcaggtaga tattccctgg ctggcaattc atcgctccgt agcgtttact tcaattgatg
    37801 cgactagcta ttctttgttg tttgcccaac attttgctaa agaatatcaa ttgtggaatt
    37861 attctgtcca aaaacaacaa ttagatcccg aacagtattt gcctcttcca gttcatccct
    37921 ggcaatggaa taacaaatta caaactctgg cctctgtatt aatcaattca aagcaattat
    37981 taataacaga ggcttttcaa aaaaccaaac catccatgtc gttccgaacg atgatgcctt
    38041 tagataacaa aggtccacac ttaaaattag ctacagcaat ccatactact tctgctttgc
    38101 gcactgtatc tcctgcatca gtaagcaatt cgtctcctct ttccagttgg cttaggcaat
    38161 tactgactca gtatcaaagt tttgaaaagc cactatttct agccagggat ttagcgggga
    38221 ttaacaccac acacccatcc atttccccac aaaataaaaa gcatttggct atgatagtac
    38281 gtgaaaatcc actggaattg atcgatgcgt cacaaacatt agtacctgta gctgcactct
    38341 atgctctttc tccagtcagt caacgtgcat tattcattga aattattgaa gcaagtggca
    38401 tggagcctga attatatttt attgaataca gccgatgtat tctcgaatgc cagcttcatt
    38461 tattattaag ctatggcgtt gcgttagaag cccaacaaca aaacacttta gtcatttttc
    38521 agtctcatcg gcctgttgga ttagttattc gagatcttgg cggcatcagg gtctgtttta
    38581 attcgctata tgagacaaca cccaaaccta atcttcatcc agactctacc atatcatgcc
    38641 acgaattgag tgatctcttt aataccttca tttatggaaa tttactgagt aacttaactt
    38701 tttgcattaa ttgtttacat gataactata acttgactaa aaaaaacctt tggatcctgc
    38761 taaaacaaat tgcaaatcaa ttattacaaa atttcaaaaa ggaaattaat ccattgatat
    38821 accaaaaatt ccaacaacat ttattctctc ttccctggca acataaaagt ttgctgagca
    38881 tgagactcaa tcaaaatcag gataacctgg tattttttag tcttgacaac ccattgagcc
    38941 aagataatga ataaaccagt tcaaaaaatg atgctctcag gtcagttttt gatgatattg
    39001 gccttggaaa tgacgaatcc ctttatacca ctattgatcg cgcaccaaag caacacacca
    39061 gtaccctcga tagtgattta taacacgctg agtctaattc taccgatgct ggctaacatt
    39121 ctattaaccc caatctgggg cttggcagct gatcgatatg gctataaacc catgttaatg
    39181 agagcatcct gggccttggt gttgacccag gccagtatga tttttgtaaa ttctgtattc
    39241 tgtattttaa tcatccgatt actgcaagga gcttttgcag gttttcttgt tgcaatgcaa
    39301 acctatgccc taagcattac taaatggcaa aacaaaagca cacaattatc tcgcttgcaa
    39361 tcctccaagg caattgccac ttctacagcc gggtttctcg gaggattagc tttaagcttg
    39421 actaactatc aaggattatt tggtttggca acattgatat gcctgggcac tactgtagcg
    39481 atgcactaca aattaccctc gccaccaaag caccagataa agaaaagatc acaaacatta
    39541 aatcattctt acacatcaaa aagtgttttc ttctttcttt gcgtattaat catgctaacc
    39601 caaattgcca aattcttacc tgatccaggg tttaccctgt atctaaataa gtattgttcc
    39661 aataatctgg tactaattgg atttctttat tctttaccag caatgggaat gctatgtagt
    39721 tcagtatggt gtggcaaaca gtttgactac tgtcgaagtc aaccttcttt ggttaaccaa
    39781 tatttaatac gctatagtgt attaggagcc atcctcatgg tcattcaagc cagtgtagat
    39841 aatttctatc tgtttggtct tatacgtatt ttgtggggga tcgtacttgc ggccttattg
    39901 cctgcattat ttgctttgtg cagtgatcgt aatttgttac caggttatgc cttgggatta
    39961 gccaattcat ttgcaaaact gggaaatcta attggattgt tattgggtgg tctttttgca
    40021 ttttatctac catatcctgc aatttttatg ataattgccg gaatttatag tttattcgcg
    40081 atttttgttt atgggtatga tcggctaaat atcaggcaga tgacagattt ttctaattct
    40141 tattttaacg tgaaatcatg aaaaaaaatg ctcttcttat agtattatta acctggtttc
    40201 ttggaaacat ggatatacat ttccttactc cagccttgcc atcattagtt gattacttct
    40261 cggtaactcc caatacagct caactaacta tcagtctttt tcttttgggg aaagccctca
    40321 gtatgatttt atggggtctt ctttccgaac gttatgggag aaaaccaatc ttcattggag
    40381 gactgttgtt atttattctg agcaattttc tgattgctct aaaccactcc atccttctct
    40441 ttttagtatg ccgatttctc cagggaatta gcgtaggcgc tacccttcta atgggtagag
    40501 caatgattaa cgatacccat aatgaacaaa atgccacaaa atactttgcg tggttattta
    40561 ctctggctgg gctgtttatt tgctttcttc cattttttgg aggtttgata aacagttatt
    40621 ggaattggca aatcgcctct cttatcattg ctgcctatgg cttgctttta ttacctttat
    40681 gtcgaggttt aaaagaaacc aaaccccatt atgtaaaatt ccctcagcta ggtcatagta
    40741 ttatggtggt cttcaaccat cccctttttg tttcttacct cctaatttct gccttgatga
    40801 tggctggaga atcagcgttt aataccagcg cctcttttat tcttattaaa ggagaaaatt
    40861 ttaccagcgc tcagtatggt acaatcaaga ccactatggc aattatgcat ttattaggta
    40921 ccgcttgttg tggcttaata gcaaaatatt atcatagtat ccaattaaca aaattaggag
    40981 tacgtttctt tattttcgca gcattcttga tgtggttatt tgtcttttct gctgaaaata
    41041 tatatttgac ttttatagtg cctatggtga tttattattt tggtacaggt tttattgttg
    41101 catcagcaac agcatcagct gttcgccctt ttcctcagca aatggctctt gcattagcta
    41161 tcactttatt ttgtcaattt aactgctccg ctttctttag ttttattacc agcatgatag
    41221 gaattcagca agtagaaact tttatgttct tattaagttt ggtaagtatt ttgagttttc
    41281 ttgcccttct aaaacttcaa cgaatggagc cctgctgaaa aaaccatgat ctttgatcct
    41341 ggtaagaaaa tataaagttt gcgtgtattt ctgcctaaag aagactatgc ttatttaaaa
    41401 taagttctta atcgtatttc gcttagaact tgtgtacttc cttcaattag aaggaaaaaa
    41461 tatggatagt aattttaaaa tcagtatacg tgtcagcatc attaccttat ttgtgctttt
    41521 gctaagcctg gtagggttca ctattattgg tattaattat attgcattca atagtgttct
    41581 gaacagtagc gctaaagatt caattgaaca aacatcatta ctcgtcaaag agaggtttga
    41641 gatctattta aatcctttaa atcaagattt aattaaaata acaaatgcca taaataatgg
    41701 catcattaac cctgatgatt caaaacaatt tgataaattt ctttttgaat caattcgata
    41761 taatcctgaa atatttatgg tttactatgg ttcaacaact ggtgatttta taggagtaga
    41821 tagagagcaa aagggaataa taggattaac ccatgtgatt aattccgtat cacctccagt
    41881 caacgttcgt tatcaattaa acgaacaagg agaaataatc ggaaaaaaat tgctaacgcg
    41941 ccattatgat ccgagagttc gcccttggta tcaacaagct ataaaagcag gtaaaccgat
    42001 atggacaaac gtatatacat tttatgtatt tgataaaagt gacttcctta tccctggaat
    42061 taccggggct tcaccaatat ataataaaaa taagcaaata aaaggagtga ttgctcttga
    42121 cctgactatt gatggtttac aacattttat ccgagaactg gaattaacta aaaacactat
    42181 gatttatgtg attaataatg agtccaatat cattgctttc agaacccctc aaaataaaaa
    42241 agacatccgt ggaaaaaaat taaccccgga atggataaaa aagctgaata ttccacttaa
    42301 aaaactggat ttcaatggtt ttgaacccat catcaaatcg tatac
//
LOCUS       DASLOE010000010        41530 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.010, whole genome shotgun sequence.
ACCESSION   DASLOE010000010 DASLOE010000000
VERSION     DASLOE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 41530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41530
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.010"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..806
                     /locus_tag="U6309_000414"
     CDS             <1..806
                     /locus_tag="U6309_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503872.1"
                     /translation="KGKILSYAVMTIISSTTLAGAMGETKTCPDLRCMPWFVEFGTGV
                     SWSTKSHINPDPSRWDPANEGYNSTLGNEPLYMAGIGYTINPLLSVDANYTFRGIYKY
                     RKHQTALGASAVNPLGNKTRYFDLISNSLMFSGTLYGQGWSENLAYSMGNYGIIQPFL
                     GGGVGVAYNTVSNFRTVTDNTNFVTSVLQDYTKASFAWQLNAGLELKKNRFGLDLGYR
                     YFNAGSFLSNNALITRVNSAGTPITTNLIPAWSSNLSANELYLTAKIAF"
     gene            948..1880
                     /gene="glsA"
                     /locus_tag="U6309_000415"
     CDS             948..1880
                     /gene="glsA"
                     /locus_tag="U6309_000415"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEN5503873.1"
                     /translation="MSSKLLTIQLLEELVHAAELNQEGKTADYIPELANVNQELTAIA
                     VQPLGEKTLAYSNNPLHPVTLQSTGKMIPLIGLLEEFGADQLFEWVKVEPSGDDFASI
                     TRLEQFGPKPSNPMLNAGAIALCSRIPGVGEQQFRWLEHWVQKLFNQRLSINPLVFAS
                     EKRTGNRNRALAYLLKSRNNLGADVHETLDLYFALCSYEAMLEQMLYLPAVLANKGQD
                     PDTGEQILSIETCKITLAIMATCGLYDETGTHMVKTGMPAKSGVSGYTIAVVPGKAGI
                     VVLSPRVNAKGNSIRGEIMLEGLSKAMNWHFALP"
     gene            2004..2891
                     /locus_tag="U6309_000416"
     CDS             2004..2891
                     /locus_tag="U6309_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HEN5503874.1"
                     /translation="MKIAVVEPIGIAIQEIRSELPGHTIIECDSRQWSDEQLIDFVKD
                     ADIIALTNRRLSAAVINSTLRLQLIAVAFAGIDHIDRDAVSKRNIPVKNAAGYANTAV
                     SELVFGLMISLARHIPDNNQRIREQGITNTGIELKNKVLGIVGYGAIGAEVARLAKAF
                     QMTTIIYDRKSQVTLDDLFSQSDFVTLHVPLTNETKGMVNLRLLSQMKKSAYLINCAR
                     GPIVVSGDLKKALEQDMIAGAALDVFDVEPPLPANYNLWEVPNLIATPHIGFNTREAL
                     VAKGQLTIRNIKEFLSSRL"
     gene            complement(3128..3727)
                     /locus_tag="U6309_000417"
     CDS             complement(3128..3727)
                     /locus_tag="U6309_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HEN5503875.1"
                     /translation="MKIVVIGATGIIGKAIVAELNPRHEIISASYKNSDIQVDITSKQ
                     SIDAMYKKLNTIDAVVLATGKVHFAALKDMGEDDYKIGLTNKLMGQVNVVLAGLEYIN
                     DNGSFTLTSGILNRDPILYGSSAAMVNGALDGFIKSAALEMPRGIRINGVSPTVITEA
                     MENYGPYFRGYEPVSAARAALAYSKSIEGAQTGQVYCVG"
     gene            complement(4057..5451)
                     /locus_tag="U6309_000418"
     CDS             complement(4057..5451)
                     /locus_tag="U6309_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEN5503876.1"
                     /translation="MVLEFDTIILGGGKGGKTLAMDLAKSGQKIAMVENNQIGGTCIN
                     VACIPTKTLVQSAKVAHYCRKAKDYGLNTTLNPIDFKAIRARKDAVVNGMREANLKQF
                     LDSGMDLMLGHGHFIGPKMIEVTLSSPRDNQKTLHITADKIIINTGALPYTPPIAGLD
                     KVNYFTNDSLMNTDSVPQHLLIIGGGYIGLEFAQMFRRFGAEVTVIEASSEFLGREDK
                     DIAEQVFQTLSNEGIQFAVDTKINAIRQEQTEVIIEANRQGQSEIIRGTAVLVAVGRI
                     ANTAGLHLDKTGVELDERGFIKVNEFLETTAAGIWALGDVKGGAQFTHLSLDDYRLVK
                     HNLQNPQKKLSSQNRLIPYTVFLDPELARIGLTEAQARSQGRPIKIATIPAAAIPRAK
                     TQGETTGVLKAVIDAETDLILGVSIFCAEAGEILAVIQLAMELRVPYQKLRDMMFAHP
                     TLVEGINLWFAHII"
     gene            complement(5503..8865)
                     /locus_tag="U6309_000419"
     CDS             complement(5503..8865)
                     /locus_tag="U6309_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEN5503877.1"
                     /translation="MMTNSWKDKLQKQLTQQFGSPKGKQLIDKYTETMSMSYCEQHTP
                     EEAIQDILQIEKLSKENSVVIDFYENTHSQYPLHIKLYRYETPIPLSDILPMLENMGL
                     RTYTERPYKIKTRDGQLFWISDFNVTYSQTDSLNITQVKDIFAEALISINLGICENDG
                     FNKLVLCAGLSWQEITILRAYAKYLHQIGIRYSQSYIEKTIEMHAAIARDLIHFFYLK
                     FGLKRKSTIHKDISALEQKIQINIDAISSLDEDRIMRYFWSLMKATLRTNYFQKNHSG
                     LSKDYLSFKLKSSEIPDLPPGQPLYEIFVYSTRFEGIHLRSTKVARGGIRWSDRPEDF
                     RTEILGLMKAQKVKNAVIVPSGAKGGFVLKKPLHAASREQIQQEVISCYKSFISGLLD
                     LTDNLINDKTIPPQNVICYDDPDPYLVVAADKGTATFSDIANSIAKEHGFWLGDAFAS
                     GGSAGYDHKKMGITARGAWESVKRHFRELDINILQQDFTVVGIGDMSGDVFGNGMLYS
                     KHIRLLAAFDHRHIFLDPNPDPLKSYEERLRLFNLPTSSWEDYNLQLISKGGGVYKRS
                     SKSIPISPQVKKALAIEANALTPNELIRALLKAPVDLLFNGGIGTYVKATTESHADVG
                     DKTNEFCRINGAELHCKVVGEGGNLGFTQLGRVEFALKGGLINTDAIDNSAGVNCSDH
                     EVNIKILLDKEIRERKLTEKKRNQLLSRMTDEVADLVLQDNYNQALILSISAAHSSHY
                     SGLYQDYMKELEKWVNLDRSVEFLPDDKKLLERKTTGAGLTRPELAVLMAHTKIHIAN
                     ELLKSHLPDDSYFTTFLETGFPISLRKPYAKALPKHPLSREIIATQLSNKLVNNMGIT
                     FMYRMLMETGMSIADIACAYTISACIFQTDVLQNLIDSFQDKISLSTQYELLHHIRQL
                     LNLATRWFLHNNRLTGGIEKNIKNYGKSVKKLEQLIPKLMGGVTKEYLEKLITQFVSI
                     GIEKDMAQKIAITRALYTSLNIIEVATQNKFDLSLTAETYFKVGSQFNLVWFRDQIAN
                     DSREGHWNTMARLSLRDELDNLQRRLTIAILTTNTKERTSEKLINYWLEQNHPIQKRW
                     EKLLEMLLGSENIDYTIFFIALRELSSLIQVL"
     gene            9063..9788
                     /gene="ceg9"
                     /locus_tag="U6309_000420"
     CDS             9063..9788
                     /gene="ceg9"
                     /locus_tag="U6309_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg9"
                     /protein_id="HEN5503878.1"
                     /translation="MYKNILFFSNFSRMRWNEYDGDRYQSLWEHNKRDIKHTFFGTIA
                     NGKDLREVLFQPVTASFSMLVAGAFAQLLVPYSIFMLCMGEAGFAGRTLINYFEITTV
                     ALAMPFIALLSTVGRCIATVKCLVEDQYNLAEMVEDKEVKPNKIYLDCAEVNYTVRDE
                     NNNQFSRKYYKFQYKMRGLNDELCQGIFTFRDLERAPDNDDFNGKLSSLTLYKYDILD
                     LIKRVHKDKFEEMEDESLIRISI"
     gene            10021..10974
                     /locus_tag="U6309_000421"
     CDS             10021..10974
                     /locus_tag="U6309_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503879.1"
                     /translation="MRLSYGLLGFMIFNLVACEKMALLTTPPKKQQLSNSPLATRAER
                     YFWRTLHEGRYQDIPQADYLLMAAYLENPYDSKLAAHLGFIHIWKITERERVKNNSPL
                     LPNEIILSKKYFADALQLDPENPIYQGFYGDTQLVEGQIFKDKQEEVRGYFTLQKAIS
                     NWPEFNYFTAGYPMSSLSADSDHFKKGLDWQWKTLDICAGVKISRQNPDFTPYMKRET
                     TIGAQRACWNSEIAPHNFEGFFMNMGDMLVKSGDPETGIKIYNNAKLAKNYNKWPYKD
                     MLEKRVLNAKANVQHFNQKSNDPNQSIMFNSGYGCVVCHQR"
     gene            11134..11475
                     /gene="arsC"
                     /locus_tag="U6309_000422"
     CDS             11134..11475
                     /gene="arsC"
                     /locus_tag="U6309_000422"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212942.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEN5503880.1"
                     /translation="MEKITIYHNPRCSKSRQALEILQNNGFEPIIIEYLKTPLDLEQL
                     KRLRTHLALEDLVRTNENVFKELGLSLANEAQVLEAMVKEPILMQRPIVTFKGKAVIG
                     RPPEKVLELFD"
     gene            complement(11562..12716)
                     /locus_tag="U6309_000423"
     CDS             complement(11562..12716)
                     /locus_tag="U6309_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEN5503881.1"
                     /translation="MMFGFLNMTLWGYLLTGFVLTHITTTAVTIYLHRYQTHRALTLH
                     PLVSHFFRFWLWLTTGMTTAEWVAIHRKHHANTDMEGDPHSPQVFNIRKVFWQVPELY
                     REASKDKDMIKKYSYGTPNDWIERKLYSPHANKGIFLMLVLNLFLFGIPGITLWAIQM
                     MWIPLVAGVVNGIGHYWGYRNHESSDSSTNVFPWGLLIAGEELHNNHHTFASSAKFSI
                     KWWEFDLGWFYIRCLSFLKLAKVKKLPPKLIIDKNKSHIDVDSVKAILGNRFQVISDY
                     YKLVIFPTLRDMQNRIKNRDLKNARHLLKKPNEQLSHADQLRLKTLLKQHDNLQAVHN
                     YRELLQQIWLKTSLSNKELLNLIQKWCSQAEESGLLVLRQFASQLKSYSL"
     gene            complement(12835..14079)
                     /locus_tag="U6309_000424"
     CDS             complement(12835..14079)
                     /locus_tag="U6309_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN5503882.1"
                     /translation="MSFKQLALIEPLNRAVSELGYTTPTSIQLKAIPLILNGHDLLGS
                     AQTGTGKTASFILPILQKASQQTQTSRNRVKVLILTPTRELAIQVHESIIQYGKYLTL
                     RSAVIYGGVKSHNQIKQLDSGLEILVATPGRLLDLYQQRAVKFDEIDTLVLDEADRML
                     DMGFIHDIKKIIKLLPLKRQNLLFSATFTPEVRALARNILNKAVEIDIAPRNTAVKTI
                     KQTVYSVDRNHKLALLSHLLHKNNWGQTLVFSRTKHGANKLVKQLAESQIYSVAIHGN
                     KSQAQRTKALADFKSGKVQTLIATDIAARGIDIEKLACVVNFDLPNVPEDYVHRIGRT
                     GRAGASGLAVSLVSTEEIKLLLSIEKLINQKLERITIKDFEFLHNFSDLVSAKIQKFA
                     PPKAGYKGNISRKSRTAFNKSA"
     gene            14411..14686
                     /locus_tag="U6309_000425"
     CDS             14411..14686
                     /locus_tag="U6309_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEN5503883.1"
                     /translation="MKQKKIYVGNLPFGTTEEALSIQFSQYGKIEEMFLIKDRLTGQM
                     KGFGFITFSAQQEAESALEMNGQPFDGRSLKVCMAQKKTNSVSRSRW"
     gene            14862..15539
                     /locus_tag="U6309_000426"
     CDS             14862..15539
                     /locus_tag="U6309_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEN5503884.1"
                     /translation="MKAKGFFNEQASLKKSKIRLNSVWMFSLGHIFLITLSNVLVQYP
                     FAMMGFHTTWGAFTYPAIFILTDLTTRIATANKARKIIMLSMTPGLILSYLVASYIES
                     GNNLNENDLSVLHLMPLRIALACFIAYVVGQLLDVAVFQRYRNRFAWWLAPTLSAVAG
                     NFIDTALFFTIAFYHCSNPFLSQNWPEIAIVDVFFKIMISLIAFVPVYGLVLSILGVK
                     TTNKLMV"
     gene            15577..16215
                     /locus_tag="U6309_000427"
     CDS             15577..16215
                     /locus_tag="U6309_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5503885.1"
                     /translation="MLTGLWLLTKDLKILNGKYIQLEPITSQHREDLRKAANHEQIWQ
                     YMPQKATGKLFNSWFDDCLEKMSTRDQITYAVRCKKEGDLKGATSFYDIQPENKRLAL
                     GYSWYIPKVWGTTTNPESKLLMLHQAFDAWGINRVELGTDSRNIHSYRAIKKLGATEE
                     GVLRQHMILHNQVITDTIMFSVLFSEWPAVKERLIQRLNYAATDKDHLAVSI"
     gene            16350..16877
                     /locus_tag="U6309_000428"
     CDS             16350..16877
                     /locus_tag="U6309_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503886.1"
                     /translation="MYNKMFKPLNTDPILYFKMYSNYNEGRVDDCCAFILMPSGLQRD
                     WVCLQSIQFAFNKCGDVLGINIIFSGNESNIHKKVRETMEGMLKLKLQYGRGEELFVF
                     DEEKKTFHLGIVPGKDTQAYLEGIIAFIKDSYRLQPDFAQDIKSQLLNKEYLAQEYSR
                     LRWKPPEKESVCVLM"
     gene            17032..18378
                     /locus_tag="U6309_000429"
     CDS             17032..18378
                     /locus_tag="U6309_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEN5503887.1"
                     /translation="MYRKQWLILLCALLNNGCLWLGSEYRKPHVNTPQKWPHKYSIQT
                     EQAAYLPDLHWWKQFNSKELNASIQKALQNNHQIHLAMANIESAQGQLQQVQLSWLPN
                     LTGLAGYTQFPVLGNPGTTAIAYPAYIINIFQQYKQQKSVQAMLEASIYAQYSARLVV
                     IAQTAASFFTLVAQNEALHLYNKLLKDYRTYLKLTQSQYRSGLISLDNITQIKSHIQR
                     IEAQIKVVQHNIVVTKNALHFLFNENPGDVETKTLFENIDSNQLVPGNLPASVLRVRP
                     DIHEAEALLKAAHADVGAITANLLPGINLGAYLGEGSNVDGAIKLGQAYLNGPIIDLP
                     LFAQVDVSKARYKAIYIKYITTIRKALRDVANDLSAYSTSSQQLNNNLSALSDEKQQC
                     HLAEVRYRHGIDDYLNLIKCQILLDEFKLVINQNKLEKLLSVVTLYQDLGGGYRGH"
     gene            18368..19405
                     /locus_tag="U6309_000430"
     CDS             18368..19405
                     /locus_tag="U6309_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN5503888.1"
                     /translation="MGIDQQKTVRTLRLSLTCIFLFLITWYYQVPESAWTLVTIWFVM
                     YEYSTVGGVLTKSFLRFAGTALSAIYGMIVVYFCANNPLINIMALVPGLFLYAYFFMG
                     GDKTYIGTIGAVTLTIVLLNYNDIDTAVLRVFNVIIGVIGSMFMIRFFYPQYARDKVL
                     EIQLNFITLLANLLESYLNPTQSLAAVQTKYLDYERKMLDGFTLYNRYIGEAKIETKK
                     APFFIPHSIAAMQHFRRLFRLFSVFVYYLSTEEIRSDPWVCDQLSKLLCNLQSLQRKL
                     LKQDEGPIGGDIMAAEPEEEIVIETPNIENKIATEAIVNNMQKEIALLDTEIKEIILI
                     YDVYDIRLNEM"
     gene            19462..20454
                     /locus_tag="U6309_000431"
     CDS             19462..20454
                     /locus_tag="U6309_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0257 family Dot/Icm type IV secretion system
                     effector"
                     /protein_id="HEN5503889.1"
                     /translation="MSDKPKAGFPVIILLCVFLFILIYHYSYLFPFTNNAFVVANVRP
                     VAANVKGYITNIYVKNEQEVKKGQPLFTVFKAPYELAYRKAVSDVQEAKAQLLLFNKQ
                     VEKTKYLLQAQKELYEKFRFDYEHNHSALRDHAVSKLTVNTMLKEKNAALSKLKALEK
                     ELEVNQQQVIVQKKKIDSLIAVMKNAKIDLDETTVYAKQNGAVQDMFVALGTPIKTRK
                     PVFAIADTDTLFIQANFNETDLRRVQPGDKVSIFPRMYFGSKIYHGVILSRNWAASRL
                     ETHRATQIQIVRNSESNWFLLPQRLPVQIQITDYDPVHYPLSIGASAYVYIHTH"
     gene            20925..21323
                     /locus_tag="U6309_000432"
     CDS             20925..21323
                     /locus_tag="U6309_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0260 family Dot/Icm T4SS effector"
                     /protein_id="HEN5503890.1"
                     /translation="MFGFFSDYKQYITLRNFAVVYNGLTGLAVLYSLWSNPEADPSEY
                     VIDISIHALTAITLMCKQAPESVKAVAMALNTYRGFDALFKAITSLPSTIPGVANAID
                     VFNHRLNFKELEKLGNEETAETRSAVQHTM"
     gene            complement(21409..22047)
                     /locus_tag="U6309_000433"
     CDS             complement(21409..22047)
                     /locus_tag="U6309_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN5503891.1"
                     /translation="MPDVLNNYYLQTMGIEVWVMRKSTPSCEKHLATLADEVASCVRC
                     SLHKTRTQTVFSRGNPKAKLMIIGEAPGFYEDQKGQPFVGKAGFLLNKMIRSIEMTEN
                     DVYIANVLKCRPPNNRDPALEEISQCSSFLTRQIKLIKPHLILALGRFAGQFLLDKPL
                     PLKQLRNHIHYYDNTPFIVSYHPAYLLRNPSDKKKAYIDWLAVKNFLVDYGQ"
     gene            22304..23579
                     /locus_tag="U6309_000434"
                     /pseudo
     CDS             22304..23579
                     /locus_tag="U6309_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101044.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            23700..24356
                     /locus_tag="U6309_000435"
     CDS             23700..24356
                     /locus_tag="U6309_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN5503892.1"
                     /translation="MKIIISILFAFISASGHAFSCYDPQTIIQKPIQFDKKRIALTRE
                     YQLTHYGIDSESIEIEPKMIVLHWTCIPSLDTTFRIFDPPALPTSSPRRNELPGDLNV
                     SSHFLVDRDGTIYQLMPETWMARHVIGLNHYAIGIENIGGVDSKDDLTEEQAKANAFL
                     VCYLKNKYPQIKYLIGHNEYLQFKGTALWLEKDPNYQTDKTDPGPTFVKKVKQLVLAS
                     "
     gene            complement(24411..25952)
                     /locus_tag="U6309_000436"
     CDS             complement(24411..25952)
                     /locus_tag="U6309_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEN5503893.1"
                     /translation="MANTIDFYPITPLAKAHWKRMSINKIKVHTLGKIFVFIMAITIM
                     ITLFNFSYATDSKLKPTVITVSEEEFQVDGKGKKTKEFKLTFTHPDGTKDNKGYFANK
                     GDMFYVLVENKSASPITLHWHGLIVPSDQDGVPDVSQIVIPPGQNKLFNYRLLQAGTY
                     WMHSHQKFQEQKQLSAPLIIYDRDDPHKDLQEVILFLEDFTYQDPQLIFDQLRTAKMD
                     MKKMDSGNDLNDVTYDAFLANKKTLAAPDVISVQPSKKVRLRIINASSSTNFKIDTGK
                     LSATLIAVDGENIEPIVDSSFPIGVGNRIDLLVEIPSAGGAFPVKAMAEGTNKQTGLI
                     LRTDNTPTPILSPNTTNKTGRVNYYELEKKLKGKNALTNKKINAHLHYVLDGQMNGYI
                     WTMNKQSWPDVTPKVIHFGDRVEMIYENKSSMSHPMHFHGHVFQVTEIDGQSLDGAMR
                     DTLLVQPHSTVKIQFDALNPGIWANHCHNLYHLNAGMFTTFEYQHYPKPDFYLKTIGQ
                     SKNNN"
     gene            26180..26614
                     /locus_tag="U6309_000437"
     CDS             26180..26614
                     /locus_tag="U6309_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503894.1"
                     /translation="MKRIFLISIIGIVLNSAYAFEHIGECGAHTWHEVASKSGSWKIT
                     LKMISQNGQFVDPSCAEIIETGLAQNNPLSYGLKFSKDADFNLAYTLTAVREEQGFQS
                     KACVFVITANGPAQPDIQALSYHGAECTWRVIKGVGENFSVG"
     gene            complement(26629..27609)
                     /locus_tag="U6309_000438"
     CDS             complement(26629..27609)
                     /locus_tag="U6309_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEN5503895.1"
                     /translation="MHSFDSIALEFDLKNHSMQKIAIEDLNIDYQNKDKIYWIHSNLN
                     QKNIFKKLREKLRLPEQVIQLCGEKDNRSTTIEIDETLTLQIQGLCSMKLNDNYDADF
                     SNLIIHLTPNYCFTASKKLSSVLYDMLKSCPKSLPYAKTSCFLLFLLLEGVINDYAKV
                     HLAYEELADQFDAQVRSPNKNTYSQVLELKHGVMKVKRYTIAIREILMRITSRNILVI
                     SEQCRTSLYNLSNHCHLIVNEIDTLRDMLNGLLGQIDNQLMQNMNETMRVLTAFAAIF
                     LPLSLITGIYGMNFYWMPELGWKYGYFGALGLIVLCALLLFLIFRKKKWF"
     gene            27888..28484
                     /locus_tag="U6309_000439"
     CDS             27888..28484
                     /locus_tag="U6309_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN5503896.1"
                     /translation="MSQDIEKIEKMYQLIMEINKLKLVFRNTITNPQRNESTAEHSWS
                     ASMITLILMNELKREFKDVDELKIIKLVLIHDVVEIYAGDVLAFDLKARKDKEKVELE
                     ALEKLSAVYPSFGIELGSLWHEFEERKSLEAKIAKAADAICPVFQRLQAKQSYIPFDI
                     SMAQLEKTKYPYFQFSKTFSNLFEKLKADLLKEQLIFL"
     gene            28619..30118
                     /locus_tag="U6309_000440"
     CDS             28619..30118
                     /locus_tag="U6309_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEN5503897.1"
                     /translation="MYDDLWKPYASTELPSLKLGGIVLNGQLLSETPIHLVLKAFNRH
                     GLIAGATGTGKTKTMQVLAEQLSLNGVPSLVMDIKGDISGLAMPGDASTKLLNRGQQL
                     HIDFTPRGFPVELLTLTPQDPGVPLRARIDDFGPLLFSLMLDLNDTQAGVVTVLFEYA
                     KANQLNLVNLDDFKSLLQFAQTDRGNQLIAAQYGGVASTSIGTIVRKIIELESQGGKQ
                     FFGEPSFVIQDLLRTDANGYGVISILRLLNMQDKPKLFSSFMLKLLSDVYRQMPELGD
                     PNKPRLVLFIDEAHLIFNYSSKALLNLIETIVKLIRSKGIGLIFCTQTPNDIPEIVLS
                     QLGLKIQHSLRAFTAKDRKALKLVSQNFPVSSYYNTEQLLTTLSIGEALVSALDAKGQ
                     PTPLVHAMIRAPESRMGPLSNEELKVLLDQSFLFGKYSQMIDAPSAKEKLAELISEQQ
                     VAKQQTSQKENNSSVLNSLSKNTLFRQVVRQVFRELTRAILSLFKSKRN"
     gene            30199..31602
                     /locus_tag="U6309_000441"
     CDS             30199..31602
                     /locus_tag="U6309_000441"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEN5503898.1"
                     /translation="MINITGTYTDQYQLAMAQVCFLKGQHKRIATFDYFFRALPFKGG
                     YAIFAGLEDLLDVLEQLRFEQQDIEFLQHQNMHPDFIHYLQQFKFNGTVYTSLEGDVV
                     FPTRPVVIIEANLIEAQIIETLLLNILNFQTLIATKARRIRQVAGERILIDFGLRRAQ
                     GAGGYFASRAAIVGGFDSTSNVRAGRDYSIPVSGTMAHSFIQSYDSEIQAFSDYAEAW
                     PENCILLVDTYNTLQSGVPNAIKIAKEMEKRGHRLKGIRLDSGDLAWLAKRARHMLDE
                     AGLNYVAITASNQLDEYVIKNLLEQDAPIDAFGVGTNLAIGAPDAALDGVYKLAFING
                     QPRIKLSDTPAKITIPYQKQVYRVFERDGTYRGADVVCLKTEKKIDMMHHPFEILKSL
                     SIKHYKKEALLHKVMENGKRLLPKKSLGEIARFSQQCFDRLPLEYKRFINPHIYKVGL
                     STELKMKRDQLIAEHSR"
     gene            31607..32227
                     /gene="pncA"
                     /locus_tag="U6309_000442"
     CDS             31607..32227
                     /gene="pncA"
                     /locus_tag="U6309_000442"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEN5503899.1"
                     /translation="MKTLIILDVQNDFMPGGSLGVPNGDAIVPVINSILHCFDLIVAS
                     QDWHPSNHTSFASNHTGRKPFEKIKLGNLEQTLWPDHCVQGSIGAQFHAQLNTNPIEA
                     IFRKGTDPNIDSYSGFYDNLHKKTTGLAGYLHEKGARELYFCGLCADICVYFTIKDAL
                     SQGFDCYLIEDATQALVKDDFIRIKKELIKQGTHIINSSNLLNNLQ"
     gene            complement(32304..32687)
                     /locus_tag="U6309_000443"
     CDS             complement(32304..32687)
                     /locus_tag="U6309_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN5503900.1"
                     /translation="MSIQLNHTIVWVKDRDKSAHFLTEILGLPEAIPYGPFMVVEMSN
                     HVSLDFCKTDKEITPQHYAFLVTETEFDDIFKRICELKMDYWADPGQTRLGQMNYNDG
                     GRGLYFNDPNGHLLEIITKPYGSGP"
     gene            complement(32687..33901)
                     /locus_tag="U6309_000444"
     CDS             complement(32687..33901)
                     /locus_tag="U6309_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5503901.1"
                     /translation="MYPKTKLSFVTLLLLISFASVNAVLFTPALPSITAFFRITEHAA
                     QQTITSFLVGYAIGQLVYGPTANRYGRKKTLYIGIGLQIISSLLCVLAGMISMYPLLV
                     LGRFLLALGSGVGLKMTFTLVNEYYEPQLAAQKTSYLMLAFAIAPGLSVAIGGILNSH
                     YGWESCFYAGTVYGAILLAMVRQLPETQKNLQLEAFQMKHLLQGYVSQFKNKPLISGG
                     ALMGGASCFVYIFAATAPFIAINLLGMSTAEYGTANIIPSLGLTIGSIWSAQFAKKYS
                     QRAGIQLGILISGFAALAMMMATLTHHSALIKLFFPMIFIYLGLSFIFANASTFAMSH
                     TSDKAHGSAVMNFINMGLVTVVILGLSLLPMSQYLLPTIYIILCILMMWLFFSFIKSN
                     EIISSIDRNKEL"
     gene            34019..34888
                     /locus_tag="U6309_000445"
     CDS             34019..34888
                     /locus_tag="U6309_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN5503902.1"
                     /translation="MGKFDQITYFIAVVEEHGFAAAARKFGVSTAAVSRQIARLEAEL
                     KAELLVRTTRKLSLTEIGARYYQQCKKTLLELNEAEIAIASSRNEAVGILNVTSSRYF
                     AMRFLLPYLPEFMELNPKLQIKIELAERFPDLAQENVDVLFGVSMEGPPELVRKRVST
                     TRYVLCASPDYLAKNGIPKTPSELGKHRYITHSMRNPDNLVTFKNNEQIYVEPVLWLN
                     DSRALCECALLGMGIIKLHEYIVAEALQDGRLIEVLPEFQESSLSVYLYYQASRYLQP
                     KIRRFIDFFTKQQ"
     gene            35302..38652
                     /gene="sdbA"
                     /locus_tag="U6309_000446"
     CDS             35302..38652
                     /gene="sdbA"
                     /locus_tag="U6309_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbA"
                     /protein_id="HEN5503903.1"
                     /translation="MHKKYNYYSLEKEKKTFWQHILDILKAPFRLPGWVVSFFLARNI
                     THVALNPNNIPQQRLIHLTKTSNRPEDDIVVINFKKRPPHKWFNDTLIKIANTIAALP
                     FVTPRLRTRLHYDNENDINHVNKLLAEIDALVQGKSKQKYCKGRVFDWSKIHLKGLEF
                     LDPKMRGYVYEQLHEKYGDVSYTTKRKPNIEFFTLKTPDGSELDSVQVTGEDEEKKPM
                     GERKFIITCIARDQNFINWIKDLNYTAKNLGATAISFNYRGVDYSRGLVWTENNLVDD
                     ILAQVQRLISLGADPKNICLDGMCIGGAVATIAAAKLHEKGMKVKLNNERSFTSLSSL
                     VFGFIVPELQTANWWSPLTYGRFLLAGVVYALLTPLIWLAGWPVDVTKAWNRIPAQDK
                     MYSVVRDKNNGLYDGVIHDHFCSIASLVDSQINSILYKLSTDQPLTEEEKQILCDDQF
                     SHHFKPSQSVLKNPKYKGPHFISRQDLVAELGHREEYTNHDYFLDRLREKFQLDRAIR
                     PVVLVEEGEKNIDGIPPQLSNHKERPLIIASSGGTGHISATHGIINDLQSKTNNVVIT
                     QHHAELYKNKPSSITSVLIRIGVWLTSLPILEDILRGVMRFIGYPVLPSSSIFWDQMS
                     KIQLGETKKENGIETGRTRPYVDMLLDIYPEGYEYTAFNNATHLTSSIEDIQTMISFK
                     GHVEEDNRNIVYQNILQRLMHAAKQNTPYTRLISTQALSLGAICDAVKYYNTVFLPVY
                     NAERGTSYQPITIDQYMTDLPSLGCIHFMNNLEELTSEQRQLMEIHAVNMSEPFKEAH
                     FGKEQGFKAVHNIDPRNNPMIRNAFKDPSLTKYLDKTQSFDLHFNVYKKEKQNALPVL
                     NGKEKITIKPHAKIASIMIGSLAANASADYAQYLLNQGYDHIFLFGGLNDSIAARIDQ
                     IINSYPDSRRDEIRKKIILLGNQSDVEMAPIMTRSNCVVIRGGGLSVMEQMAMPIMDE
                     KIVLLHHEDNEEGPLTSGLSWEDGNADKLIEYLSEKGAYAKKTSPGLCSGHLNEAEKD
                     FEKKYITQLKSTGTKEKVDLTIPQQKKPPLKKEWGRKTGYTELGQVLSHQHRFFETIP
                     EVREPFCSKEDLHHNELSSQSLVSVTVG"
     gene            38825..40327
                     /gene="legG2"
                     /locus_tag="U6309_000447"
     CDS             38825..40327
                     /gene="legG2"
                     /locus_tag="U6309_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegG2"
                     /protein_id="HEN5503904.1"
                     /translation="MRNKSNLLPLIACFNNREINMSKILDSNAYREMENELNNLFEQI
                     YRHGKNDRTAITLQAQQFLDNLSESIRDHSRLLLTKIAREELIYFKEKKPGDSREKFQ
                     NIDKMSDYFNNLSALINFSVMQQEDPTLRVFYYDMYIQLMNFCYLKGDLFGAGAIYTG
                     LIANNLPNSIDWKKLSSESRLIFKDCEEKFKRLFNLSTTYNAHTDLKRQFKTALIPAL
                     PSLLTVQIYTKDSYDNSVSDFERIQDKLRQLDIEHNQFLNQLQNDHSTWSKAYYDYMS
                     NVYLPQSILEYTTLLREYEELVTTHGGPIAKQFHKDKNSEPLVNFVESTLSENQSVLK
                     VSPFQNERIVEMLSEIDVVKSECSSPAVFDDLSWKLREMNCTLVKAKSIGDYTSKIFA
                     NLDTPYMEENTTDEDPKPVLVEELQTDDVYQLHYSSMKLMRELEQAIEDHKPKVIYHC
                     SGEANYGFFKEARRRGRHLSPEPMDIEVMDMDVMDYDENDLRDQTVMITV"
     gene            complement(40393..>41530)
                     /locus_tag="U6309_000448"
     CDS             complement(40393..>41530)
                     /locus_tag="U6309_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEN5503905.1"
                     /translation="LSHEAGDQLLCAVAKRLRSLVRKEDTLARLGGDEFVMLFQSFNS
                     EEQIAGVAQKILKSFQKPFHIAERDINIHVSIGISVYPTDGKTVNTLLSNADMAMYQA
                     KFRGGNQFSFYTEKLNKKTNQQFQLELDLQKAIIHHEFFLLYQPQFTVNTHEIRSMEA
                     LIRWNHPQKGIILPLDFIPTAESSGLIVPIGEWVLRETCKQIGTWRTMGLPDIRVACN
                     VASKQLRQRDFPAMVEDLLKEYSVEPHCLELEITENVIIDKEIQRTIKKLKSLGVLII
                     LDDFGTGNSSLNLLKQVAVDSLKIDQSFIQNISKSPGDEAIIDAIIAIAQSMNFNIIA
                     EGVETQNQLKFLKKRRCNDIQGFLMSKPIPPEEIEKLLQGNRTK"
BASE COUNT        12547 a         7494 c         8492 g        12997 t
ORIGIN      
        1 caaagggtaa aattttaagt tacgctgtaa tgacaatcat atcgagtact acattggcag
       61 gagcaatggg tgaaaccaaa acatgtcctg atctcaggtg catgccttgg tttgtagaat
      121 ttgggactgg tgtcagttgg tctactaaat ctcatattaa tcccgatcca agtcgttggg
      181 atcctgctaa tgaaggttat aacagtactt taggtaatga acctttatat atggcaggta
      241 tagggtatac aatcaatcca ttgctctctg ttgatgcaaa ctatactttc cgaggaattt
      301 ataaatatcg caaacatcaa actgctcttg gagccagtgc tgtgaaccct ttaggcaata
      361 aaacgcggta ttttgattta ataagcaact ccttaatgtt tagtggtact ttatacgggc
      421 aaggatggtc agaaaatctt gcgtattcaa tgggtaatta cggtatcatt cagccttttt
      481 tgggtggtgg agtcggcgtc gcttataaca ctgtcagtaa ctttcgtacg gttacagaca
      541 atactaattt tgtgactagt gtattgcaag attacacaaa agcatctttc gcttggcaat
      601 taaatgctgg tttggaattg aaaaagaata gattcggcct tgatttagga tatcgttact
      661 ttaatgcagg ttccttttta tctaataatg cgttgattac ccgggtaaat agtgcgggga
      721 cacctatcac tacgaatctt atcccggcat ggtctagtaa cttatcagca aatgaacttt
      781 atttgaccgc caaaattgct ttttaataaa atacaggatg atttaattaa tcatgttaat
      841 tattgatcat ccttagccta tttattagca atatcctttc aaaataaaat atttgtctta
      901 atgtgctaaa tttaccataa tagccccttt tttaatccaa tagtactatg tcatcaaaat
      961 tgcttaccat ccaattgtta gaagagcttg tgcatgctgc cgaattaaat caagaaggca
     1021 aaaccgcgga ttatatccct gaactggcga atgttaatca ggaattgaca gcaattgcgg
     1081 tgcaaccttt gggtgagaaa accttggctt atagtaataa tccacttcat ccagtgactt
     1141 tgcaaagcac tgggaagatg atccctttga ttggtttgct tgaggagttt ggtgctgatc
     1201 aattgtttga atgggttaag gtggagcctt cgggcgatga ttttgcttcc attacccgcc
     1261 tggagcagtt tgggcctaaa ccgtcaaatc ccatgctgaa cgctggcgca attgccttat
     1321 gttcacgtat tccaggcgtg ggtgagcagc agtttagatg gttagaacat tgggtacaaa
     1381 aattatttaa tcaacgtctc agtattaatc ctctggtttt tgcctctgaa aaacgaactg
     1441 gtaatcgtaa ccgcgctctt gcctatttac tgaaaagcag aaacaatctt ggagctgatg
     1501 ttcatgaaac cctcgatttg tattttgcgc tatgttccta tgaagccatg ttagaacaga
     1561 tgctgtactt acctgctgtg ttagcgaaca aggggcaaga tccggacacg ggtgaacaaa
     1621 tcctttctat cgaaacctgt aaaattactt tggctatcat ggcaacctgt ggattatatg
     1681 atgaaacagg tactcacatg gttaaaacag ggatgccagc taaaagtgga gtgtctggtt
     1741 ataccatcgc ggttgttccc ggtaaagcag gtattgtggt gttgagtcca agagtgaatg
     1801 cgaaaggaaa tagcatacga ggtgaaatca tgctggaagg tttatccaaa gcaatgaatt
     1861 ggcatttcgc attaccttaa tttttaaaaa agagcgttat agaatggtga gctatctctt
     1921 tgattgaagt gaaataacga tgacaaattg cttgtcacag cggagtggaa aagcttaatt
     1981 aaatttggat taaaggatta tacatgaaaa ttgccgttgt tgagcctata gggattgcga
     2041 ttcaggaaat tcgttctgaa ttaccaggcc acacgattat cgagtgtgac agtcgtcagt
     2101 ggagtgatga gcaattaata gattttgtga aggatgcaga catcattgca ctgacgaatc
     2161 gacgtttatc ggctgcagtc attaattcga cgctgcgatt acaattgatt gctgttgcat
     2221 ttgctggaat cgaccatatt gatagggatg cggtgagtaa aagaaatatt ccggtaaaaa
     2281 atgcagcggg ttatgccaac actgcggtat ctgaactggt atttggtctc atgatttctc
     2341 ttgcacgtca tattcctgat aacaatcaga gaataagaga gcaaggcata accaataccg
     2401 gtattgaact aaaaaataaa gtgctgggta ttgtcggtta tggagcaatc ggcgcagaag
     2461 ttgcgcggct tgctaaggca tttcaaatga caactataat ttatgacagg aaatctcaag
     2521 taacattaga cgatttattt tcccaatctg atttcgtcac tttacatgtg ccacttacaa
     2581 atgaaaccaa aggcatggtt aacttaagac ttttatctca aatgaaaaaa tcagcgtatt
     2641 tgattaattg tgccagaggg ccgattgtgg ttagcggtga tttaaaaaaa gccctggaac
     2701 aggacatgat agcaggtgcc gcattggatg tatttgatgt tgaaccacca ttacctgcaa
     2761 attataacct ttgggaggta cctaatctta tcgcaacacc acacatagga tttaatacaa
     2821 gggaggccct tgtggcgaag gggcaattga ctatcagaaa tatcaaagag tttctttcgt
     2881 cccgtctata aaaccgtgta aaaacataaa gaaacataag aagttgattg caaaatagct
     2941 tgtagtactt ctgaagtaat ctaaactggg gcaaaattat gctttaacgt aatggtaggg
     3001 tccataccat tacgttattt tacctgggta aaaaaatttt taattcggtc atttagttgc
     3061 tcttgactcc ctcgttaaaa acattttttt gcctcgacct tggggttttt cataagtcct
     3121 gttaatgtta ccctacgcaa tagacttggc ctgtttgagc gccctcaata ctcttgctgt
     3181 aggcgagtgc tgcacgggca gcggatacag gttcatagcc tctgaaatag ggaccatagt
     3241 tttccatggc ttcggtaatt actgtggggc ttacaccatt gattcggata cctcttggca
     3301 tttccagagc agcacttttg ataaaaccat ccaatgcgcc attaaccatg gcagcggaac
     3361 ttccatagag aatgggatca cgatttaata ttccgcttgt taatgtaaat gagccattat
     3421 cattgatgta ttctaatcca gccaagacta cattaacttg acccattagt ttattggtca
     3481 aaccaatttt ataatcgtct tctcccatgt cttttagggc agcaaagtgc actttaccgg
     3541 tagctaacac aaccgcatca atagtattta attttttgta cattgcatcg attgattgtt
     3601 tactggttat atccacttga atatcactgt ttttatagct cgctgaaata atttcatgtc
     3661 tcgggtttaa ttcagcaact attgcttttc caattatccc cgtagcacca atcaccacta
     3721 ttttcataat tcattctctc tttatccaat aaaaagcaaa agtcctatgt agaatagtac
     3781 agtaacaaca atgagttaaa tacaggactt acgaaaaccc caatctctat gttaaaaaaa
     3841 gtttttaatt cggtcattta gtttatctaa actctctcat taaaaacatt ttttgccttg
     3901 accttggagt ttttcgtaag tcccgaaata aaaagtgtgt accggctgta tttgattact
     3961 gcctatacac aggatttatt gaagaagtga agtaaaatgc ctctattaac cacttgcctt
     4021 gtagcatgta atatggcgag tatagtttac atcaattcaa attatgtgcg caaaccacag
     4081 attgatgcct tccaccagag tagggtgagc gaacatcata tcgcgcaatt tttgataagg
     4141 tacgcgcaac tccatagcta attggatgac cgctaatatt tcccctgcct ccgcacagaa
     4201 aatagaaacg cccaaaatca aatcggtttc agcgtcaatg actgctttta aaacacccgt
     4261 cgtttctcct tgggttttgg cgcgaggaat tgcagctgcc ggaattgtgg ctattttaat
     4321 tgggcggcct tgggatctgg cttgagcttc agtaagtcca atgcgtgcta attccgggtc
     4381 gagaaacact gtgtaaggaa taagcctgtt ttgggaagaa agctttttct gggggttttg
     4441 taaattatgt ttcaccagac gatagtcatc aagagacaag tgggtaaact gtgcccctcc
     4501 cttgacatcg cccaatgccc aaatccctgc tgctgtcgtt tcgagaaact cattgacttt
     4561 gatgaatcca cgctcatcaa gttcaacgcc agttttatcc aaatgcaatc ctgctgtatt
     4621 ggcaatacgc cctactgcaa ccaggacagc tgtgccacga ataatttctg attggccttg
     4681 tcgattagct tcgataatca cttcagtttg ctcttggcga atggcattaa tctttgtatc
     4741 gacagcaaat tgaatgcctt cgttggataa agtttgaaaa acctgttcag caatgtcctt
     4801 gtcttctcgg ccaagaaact cgctgctggc ttcgatgacg gtgacttcag cgccaaaccg
     4861 gcgaaacatt tgcgcaaatt ccaagccaat ataaccgccg ccgatgatca ataaatgttg
     4921 gggtaccgaa tcggtgttca ttaaactgtc attggtaaaa taattgactt tatcgagccc
     4981 cgcaatgggg ggagtgtaag gcagggcacc tgtgttaata ataattttat cagctgtgat
     5041 gtgcagtgtt ttttgattgt cacgtggtga ggatagggta acttcaatca tttttggtcc
     5101 aataaaatga ccatgtccta acatgagatc catgcccgaa tctaaaaatt gttttaaatt
     5161 cgcttcacgc atcccattca ccacagcgtc tttacgtgct cgaattgctt taaaatcaat
     5221 tgggttcaat gtagtattta aaccatagtc ttttgcttta cgacagtaat gtgctacttt
     5281 tgctgactgc acaagcgttt tcgtaggtat gcatgccaca ttgatacagg tcccaccgat
     5341 ctgattgttt tcaaccatcg cgattttctg gccgctttta gccaaatcca ttgcaagagt
     5401 cttcccacct ttgcccccac ctaatatgat ggtatcaaac tctaaaacca ttctattctc
     5461 ccgaaaattt caatgtttta tccacttgat gatgatccat atttacaata cttgaattaa
     5521 agaggaaagc tcacgcaagg cgataaaaaa tatggtgtaa tctatgtttt cgctacccaa
     5581 taacatttct aataactttt cccagcgttt ttgaatgggg tgattttgtt ccagccaata
     5641 gtttatcaat ttttcagaag tgcgttcttt ggtgttagtt gttaaaatgg caatagtcaa
     5701 acgtctttga agattatcta actcatcacg caaagacaga cgagccatgg tattccagtg
     5761 gccttcacgt gagtcattgg ctatttggtc gcgaaaccaa acgagattaa attgactgcc
     5821 aactttaaag taagtttctg cagtaagcga taaatcaaat ttattttgcg tggctacttc
     5881 aatgatattt aatgaagtat aaagagcccg ggttatagca attttttgag ccatatcttt
     5941 ttcaatgcca atactgacga attgggtaat gagtttctca aggtattctt tagtgacacc
     6001 gcccatgagc ttgggaatta actgttcaag ttttttgact gactttccat aatttttaat
     6061 atttttttca atgcctccag tcagtcgatt attatgcaaa aaccagcgtg tcgccaggtt
     6121 gagcaattgg cgaatatgat gcaataattc atattgggtg gataaggata ttttatcttg
     6181 aaatgaatca atcagatttt gtaagacatc cgtttggaaa atacaggctg aaatagtgta
     6241 agcgcaggca atatcagcaa tcgacatgcc ggtttccatc agcatgcggt acatgaaggt
     6301 aattcccatg ttgttaacca gcttattgct caattgagtc gctatgattt cacgagacaa
     6361 agggtgtttt ggcaaggctt tcgcataggg ctttcgtagc gagatgggaa aacctgtttc
     6421 caaaaaagtt gtaaaatatg aatcatcggg taaatgcgat tttaataatt cgttagcaat
     6481 atgaattttg gtgtgtgcca ttaaaacagc aagctctggt cttgtcagtc ctgcgccagt
     6541 ggttttgcgt tcgaggagtt ttttatcgtc aggaaggaat tcaacgcttc tgtcaaggtt
     6601 aacccacttt tccaattctt tcatgtaatc ttgatataag ccactataat gagaagaatg
     6661 agctgccgat atacttaaaa tcaaggcttg attgtagttg tcttgcaaaa caagatcggc
     6721 gacttcgtct gtcattctgc ttagcaattg attgcgtttt ttttctgtga gcttcctttc
     6781 tctaatctct ttatctaaaa gaattttgat attgacttca tggtcagagc agttaacgcc
     6841 ggcggaatta tcaatcgcat cagtgttaat aagaccgccc tttaatgcaa actcgacgcg
     6901 tccaagttga gtaaaaccca gatttcctcc ttctcccact accttgcagt gtagttctgc
     6961 gccattaatg cggcagaatt cattagtctt gtcgccaaca tcagcatgtg actccgtggt
     7021 tgctttgaca taggttccaa taccgccgtt aaatagtaaa tccactggtg cttttaacag
     7081 tgctcgaata agctcgttgg gcgtaagggc atttgcttct atagcgagag ctttttttac
     7141 ttgcgggcta atggggattg atttgcttga gcgtttgtaa acgcccccgc ctttagaaat
     7201 aagttgtaag ttgtaatctt cccaggacga agtaggtaaa ttaaacaggc gaagtctttc
     7261 ttcataggat tttaaagggt cgggattcgg gtcgagaaaa atatgacgat gatcaaaagc
     7321 agctaacagt cgtatatgct ttgaataaag catgccattg ccaaatacat caccactcat
     7381 atcaccaata cctactaccg taaagtcttg ttgcagaata ttgatatcaa gttcacgaaa
     7441 atgtcttttg acagattccc acgcaccacg tgctgtaata cccatttttt tatgatcgta
     7501 cccggcagaa cctccagaag caaaagcatc acctagccaa aatccatgtt ccttggcaat
     7561 actattggca atatcggaaa aagtagctgt tcccttgtca gcagcaacaa ctagataagg
     7621 gtcagggtcg tcgtaacata taacattctg tggagggatt gttttatcat tgattaaatt
     7681 atcggtgaga tccagtaaac cactgatgaa cgatttataa caagagatga cttcctgttg
     7741 gatttgttcg cggctggctg catgaagcgg ctttttcaag acaaaacctc ccttggcgcc
     7801 agagggaaca atgactgcgt tttttacctt ctgcgctttc ataagcccta aaatctcggt
     7861 tcgaaaatct tctggccgat cggaccagcg aataccgcct cgggctactt tggtactacg
     7921 taaatggatt ccttcaaaac gtgttgagta cacaaaaatt tcataaagcg gttgacctgg
     7981 aggtaagtca ggaatttccg aggattttaa tttgaaggaa aggtaatctt tagataatcc
     8041 cgagtgattt ttttgaaagt aattagtacg aagagttgct ttcatcaaag accaaaaata
     8101 acgcatgatg cgatcctcat ccaggctgct aatcgcatca atgtttattt gaattttctg
     8161 ttcgagtgcc gatatgtcct tgtgaatagt agatttcctt ttaaggccaa atttcaaata
     8221 aaaaaagtga atcagatcac gggcaatggc cgcatgcatt tcgatggttt tttcaatgta
     8281 agattggctg taacgaatgc caatctggtg gaggtatttt gcataggctc gcaaaatagt
     8341 aatttcttgc caggagagtc ccgcgcataa caccaattta ttaaaaccat cgttttcaca
     8401 aataccaaga ttaatactaa tcagtgcctc tgcaaaaata tctttgactt gagtaatgtt
     8461 gagtgaatcg gtttgagaat aggttacatt aaaatcacta atccagaata attgcccgtc
     8521 ccgtgttttg attttataag gacgttcggt ataagtgcgc agtcccatgt tttcaagcat
     8581 aggcaagatg tcggataagg gaattggggt ttcataccga taaagtttaa tgtgcaaagg
     8641 atattgtgag tgagtgtttt cataaaaatc gataaccact gaattttctt tggacagttt
     8701 ttctatctgt aaaatgtcct ggattgcctc ttcgggagta tgctgttcac aataactcat
     8761 tgacattgtt tcggtgtatt tatcaatgag ctgtttccct tttggggagc caaattgctg
     8821 agttaattgc ttttgcaatt tatctttcca tgagtttgtc atcattctta aaattccatg
     8881 caagatatgt ttttatcgtt actcaaaagt ataggccatt tcaaagaata aaattaaaat
     8941 ttgctaactt atctcgcaca aagtgaatca tttgttttca ggaaattatt aagtaagctt
     9001 taataaaatg ttaagggcat aaaagtataa taaagcttct ttcaaaagac gaagcaccta
     9061 ttatgtataa aaatatactg tttttttcaa attttagccg tatgcgttgg aacgagtacg
     9121 atggggatcg atatcaaagt ctttgggaac acaataagcg agatattaaa cacacatttt
     9181 tcggtacgat tgcaaacgga aaagatttgc gagaagtgct attccagcca gttactgctt
     9241 ctttttcgat gctagtcgcg ggggcatttg cacaattact ggtgccctat tccattttca
     9301 tgctctgtat gggtgaggct gggtttgcgg gcagaacatt aattaattat tttgaaataa
     9361 ctactgttgc tttggcaatg ccatttattg ctttattaag tactgtaggc cgatgtattg
     9421 caacggttaa atgcctggtt gaagatcaat acaatttggc tgagatggtt gaagacaaag
     9481 aggttaagcc aaataaaatc tatcttgatt gtgctgaagt aaactacact gtcagggatg
     9541 agaacaacaa tcagttcagc cgcaaatatt ataaattcca atataaaatg agaggtctca
     9601 atgatgagct ttgccaggga atttttactt tcagagattt agagcgtgct cctgataatg
     9661 acgatttcaa tggaaagctt tccagtctca cactttataa atacgacatc ctggacttga
     9721 ttaaaagagt tcataaagat aaatttgaag aaatggaaga cgaaagtcta attcgtatat
     9781 ccatttaatt tagattggtt agaaaatcag gctgtttttg gctggaagct gtaatggaga
     9841 gtgattattt ttctcaacta cttgccactt aagaggccac gttatatcgt ttatgaatga
     9901 tatgattggt tcctgcctat atttttgatg cgctgacttg gtaatgagtt attgagttgg
     9961 cgtctaaagc aagacaaaac atagggatga aataaataaa aggattaagg gattaagagc
    10021 gtgcgtttat cttatggctt gttaggtttc atgatattta atttggtggc ttgtgaaaaa
    10081 atggcattac tgactacgcc acctaaaaaa cagcaattat ctaatagtcc actggctact
    10141 cgggcagagc gatatttttg gcgaaccctt catgaggggc gatatcagga cattccccaa
    10201 gcggattatt tgctgatggc agcttacctc gagaatccgt atgattcgaa attagcagcc
    10261 caccttggtt ttattcatat ttggaaaatt acggagcggg aaagagtaaa aaataactct
    10321 cccttgcttc ccaatgaaat tattttgtct aaaaaatact ttgctgatgc tctgcaactg
    10381 gatccggaaa acccaattta tcaaggattt tatggcgata cccaattagt agaagggcag
    10441 atttttaaag acaaacaaga agaagtcagg ggatacttta ctttacagaa agcgataagc
    10501 aattggcctg aatttaatta ttttactgca gggtacccca tgagtagttt aagtgctgat
    10561 tcggatcatt ttaaaaaggg tttggattgg caatggaaaa ctttggatat ctgtgctggt
    10621 gtgaagatta gtagacagaa tcctgatttt acgccttata tgaagaggga aacaacaata
    10681 ggcgcgcaaa gagcctgttg gaattcagaa attgcgcctc ataactttga aggttttttt
    10741 atgaatatgg gggatatgtt agttaaatcc ggagatcccg aaacaggaat aaaaatatac
    10801 aataatgcca aattggctaa aaattacaat aaatggccgt ataaagatat gttggagaaa
    10861 cgtgttctga atgcgaaagc caatgtgcaa cattttaatc aaaaatccaa tgaccccaat
    10921 cagtcgatta tgtttaattc gggatatgga tgcgtggttt gtcatcaacg gtaagtacca
    10981 tctgattatt ctatatcagg tggcttatta gattagtaag agtgtgaggt ttcagtttaa
    11041 aactcctgct tgtgtttttg caaagcgggc aggataaaag cgaagaaaag tgttatagtt
    11101 ttaatttatt tcggcaaaga atttaaaaag aacatggaaa aaattactat ttatcataat
    11161 cctcgatgct caaaatctag acaagcattg gaaatactgc aaaacaatgg ttttgaaccc
    11221 attatcattg aatatcttaa aactcctctt gatctggaac aattgaaaag attacggact
    11281 cacctggctc ttgaagactt ggtgcgcacg aatgaaaatg tttttaaaga attgggtctg
    11341 tctctggcta atgaggcgca agttttagaa gctatggtaa aagaacctat attaatgcaa
    11401 cgcccaattg tcacatttaa aggtaaggct gttatcgggc gtccgccaga gaaagtcctg
    11461 gagctgtttg attaatcttt attgtacgat aatataaatt taatcctgat accatgacta
    11521 atctcactgt catggtaagg aataaaagca tttgtaaaac ttcagagaga atagcttttt
    11581 aattgtgatg caaattgcct taacacaagc agtccggatt cttcggcctg gctacaccat
    11641 ttttgtatta aattaagaag ctccttattg gatagggacg tttttagcca tatctgctgt
    11701 aataattcgc ggtaattatg aaccgcttgc aagttgtcat gttgcttaag taatgttttc
    11761 aatcgcaact gatcagcatg ggatagctgt tcgttcggtt tcttgagtaa atggcgagca
    11821 ttctttaaat cccgattttt tatgcggttt tgcatatcgc gcagagtagg aaatataacc
    11881 agcttgtaat aatcagatat cacttgaaag cgattgccaa gtatggcttt taccgaatct
    11941 acatctatat gagatttatt cttgtcaatg atcagttttg ggggtaattt ttttactttg
    12001 gcaagcttta aaaaagacaa acaacgtatg taaaaccaac ccaaatcaaa ttcccaccat
    12061 ttaatggaaa atttggccga ggaggcgaag gtatgatgat tattatgcaa ttcttcacca
    12121 gcaatcaata aaccccaggg aaaaacattg gttgaagaat ctgacgattc atgattgcga
    12181 taaccccaat aatgccctat tccattgacc acgccagcaa caagaggtat ccacatcatt
    12241 tggattgccc aaagggtaat tccaggaatt ccaaacaaga aaagattaag tacgagcatt
    12301 aaaaatatgc ctttgttcgc atggggagag tataatttgc gctctatcca gtcgttgggg
    12361 gtaccatagg agtacttttt aatcatgtct ttatctttag aggcttctcg gtataactct
    12421 ggaacctgcc aaaatacttt tcttatatta aatacctgag ggctatgcgg atccccttcc
    12481 atgtcagtat tggcatgatg tttccgatga atcgctaccc attccgctgt ggtcatcccg
    12541 gtcgttaacc aaagccagaa acggaaaaaa tggctgacaa gaggatgcag agttaatgcg
    12601 cggtgtgtct ggtagcgatg caggtaaatg gtaacagcgg tggtggtgat gtgagtcagg
    12661 acaaaaccgg tcaaaagata tccccataaa gtcatattta aaaaaccaaa catcatttct
    12721 cctctttggt gcctaaaatt gtgtaattgg caaaacgata tgtaagaata tctttatatc
    12781 cactaagtat atatcattta tttttatctt agtggcattt aaaaagctaa ttatttacgc
    12841 gctcttatta aaagccgtcc tggattttct gctgatattg cccttatatc ccgcttttgg
    12901 cggcgcaaat ttttgaattt tggctgagac taaatctgaa aagttatgca aaaactcaaa
    12961 atccttaatt gtaatgcgct ccagtttttg attgattaat ttttctatag acagcagcag
    13021 cttgatttct tctgtgctga ccaaggatac agccaatcca gaggcgcctg cccgtcctgt
    13081 tcgtccaatc cgatgaacgt agtcctcagg cacattaggc aggtcaaaat tcactacaca
    13141 ggcaagtttc tcgatgtcta tgccacgtgc tgcgatgtct gttgcaatta atgtttgtac
    13201 tttgcctgac ttgaaatcag ccaaggcttt tgttcgttga gcctgtgatt tattgccatg
    13261 aatagcaacc gaatatattt gggattcggc cagttgctta accagtttat tggctccatg
    13321 tttggttcga gaaaaaacca aagtttgtcc ccagttattc ttatgaagta aatgactcag
    13381 aagggctaac ttatggtttc tatctactga ataaacagtt tgttttatgg tttttaccgc
    13441 ggtatttctc ggtgcgatat caatttcaac cgctttattc agtatatttc tggctaaagc
    13501 gcgcacttca ggggtaaagg ttgctgaaaa aagcaggttc tgccttttta aaggtaataa
    13561 tttgataatt ttttttatat catgaataaa ccccatatcc agcattctat ccgcctcatc
    13621 gagtaccagg gtgtcaatct catcaaattt aacagcacgt tgctgatata aatctaataa
    13681 tcttcctggt gttgcaacca gtatctcaag gcctgagtct aattgtttaa tttggttgtg
    13741 gctttttact ccaccgtaaa tcacagccga gcgcagtgtt aggtatttgc catattggat
    13801 tatgctctcg tggacctgga tggctaattc tcgtgtgggc gttaaaatca gtactttaac
    13861 tcgattacgg ctggtttgag tttgctgact ggctttttgc aagataggca atataaagct
    13921 tgctgttttt cctgttcctg tttgagcgga accaagtaaa tcatgcccat ttaaaatgag
    13981 cgggatggct tttaattgaa tactggtagg ggtagtgtag cctaactcac taaccgccct
    14041 gtttagcggc tcaatcagcg ctaattgttt aaaactcatt gttttcctta ttgatatcta
    14101 ttggtattgt gggctctgag tttaatcact cattatttta tggggaaaat gaggcattaa
    14161 ttagaggggg aaaaatagga attttcaaag gttttttgaa agaattcatg caatattagg
    14221 aactattata acagttattt gtaaatatgt cttgtttatt ttacttaggc ttggattgtt
    14281 aattaacagg taacatgcta agctcctcag tagatttaat tctacgcaca atactctttt
    14341 cgatcgtttg atttttcact cttcttgctt tcctccagat cattgttcgt taatctattc
    14401 aggagcaatg atgaaacaaa aaaaaatata tgttggtaat ttaccatttg gtacgactga
    14461 agaagcatta agcattcaat tttctcaata tggaaaaatc gaggagatgt tcttaattaa
    14521 ggatcgatta acaggacaaa tgaagggatt tggttttata acgttttctg ctcagcagga
    14581 agcggaatct gcgttagaga tgaatggtca gccgtttgat ggacgttcat taaaagtatg
    14641 tatggcacaa aaaaaaacca attctgtttc tcgtagccgg tggtaaaata gagtatttac
    14701 ttaattccga tgttatcgta tcgagaactc atcactacag ataaattttt aatctacgtg
    14761 ggctcaactt ttttagtccc gagtgagcca cttattttct tgtgttttat ctaccccata
    14821 tgcaaaaata acaaccatag tatttggtga taatagtcag catgaaagcg aaaggttttt
    14881 ttaatgaaca agcttccctg aagaaaagta aaattaggct gaattcagta tggatgtttt
    14941 cacttggtca tatttttcta ataacgctca gtaatgttct ggttcaatat ccatttgcta
    15001 tgatgggatt tcacacgacc tggggggcgt tcacttatcc agcaattttt attttaactg
    15061 atttgacaac aaggatagca accgctaata aggcgcggaa aattattatg ctgagtatga
    15121 ccccaggatt aatattgtcc tatcttgttg cgagctatat tgagtcaggc aataatttga
    15181 atgagaacga tctatccgtt ttacacctca tgccattacg tatcgccctg gcttgtttta
    15241 tagcctacgt tgttgggcaa ttgctggatg tggcagtttt ccaacgctat agaaatcggt
    15301 ttgcctggtg gctggcccca actctatcgg cggttgcagg caatttcata gatacggcat
    15361 tattttttac catcgcgttt tatcactgct ccaatccatt tttaagtcag aattggcctg
    15421 aaattgcgat agtcgatgtc ttttttaaaa tcatgattag tttaattgct tttgttccgg
    15481 tttatggttt ggttttaagt attcttggtg taaaaactac caataaatta atggtataaa
    15541 ggtatcttga caccattttc aaaataagga ttgatgatgc tgactggttt atggctgctt
    15601 acaaaagacc tcaagatttt aaatggcaaa tacattcagc tagaacccat taccagccag
    15661 caccgtgagg acttacgaaa agcagcaaat catgaacaaa tttggcaata catgccgcaa
    15721 aaggcaacgg gcaagttatt taattcctgg tttgatgatt gtcttgagaa aatgtccacg
    15781 agagaccaaa ttacttatgc agtgcgttgt aaaaaagaag gggatctgaa aggggccact
    15841 tcattttacg atattcaacc agaaaacaaa cggttggctt taggctatag ttggtacatt
    15901 cctaaagtat ggggaacaac gacaaacccg gagtcaaagt tactcatgtt gcatcaggcg
    15961 tttgatgcct gggggattaa tcgagtagaa ctgggtacag attctcgcaa tattcattcc
    16021 tatcgtgcta tcaaaaagct gggtgcgacg gaggaagggg tgttgcgcca gcatatgatt
    16081 cttcataatc aggtgataac ggatactatt atgtttagtg ttcttttctc ggagtggccg
    16141 gcggtaaaag agcgattaat acagagattg aactatgctg caaccgataa agaccacttg
    16201 gctgtctcga tatagtaatc ccttgttctc tgggttaaac agctcaactc atgaaataat
    16261 cagacacaca ataaatagaa tgaaaaaaga ttggtatttt tctttaattg agtgaatatt
    16321 tggttctacc tgcgagaaag gagtgctcaa tgtacaacaa aatgtttaag cctctgaata
    16381 ctgatccgat tttatatttc aaaatgtaca gtaattataa tgagggaagg gttgatgatt
    16441 gttgtgcatt tattttaatg cccagtgggt tacaaagaga ttgggtctgt ttgcaatcga
    16501 ttcaatttgc ttttaataag tgtggtgacg tccttgggat taacattatt ttttctggaa
    16561 atgagtcgaa tatccacaaa aaggttcgag aaacgatgga agggatgctc aagctgaagc
    16621 ttcaatatgg aagaggggaa gagcttttcg tttttgatga agaaaagaaa acatttcatc
    16681 tggggatagt gcctggtaag gatacacagg cgtatttgga gggcattatc gcttttatta
    16741 aggatagtta tcgcttgcaa ccggactttg cccaagacat caagtcacaa ctacttaata
    16801 aggaatatct ggcacaggaa tatagtcgtt tgcgatggaa gccaccagag aaagaatctg
    16861 tttgtgtgct catgtaggtt ctagaagtat tgtttccact ttaaaaatag aaaaatttga
    16921 attttatggg gattattgca ttccattagc ctggtttgcg taatactctc ttgacaacta
    16981 caagattgaa agctcaatat tctttattta caccaaggtt caggattttc attgtatagg
    17041 aagcaatggc tcattttatt gtgtgctttg ttgaataatg gttgcctctg gcttgggtca
    17101 gaatacagga aacctcatgt caacaccccg caaaaatggc cgcataaata ttcaattcaa
    17161 actgagcaag ctgcctattt acctgatctg cattggtgga aacagttcaa tagcaaggag
    17221 ctcaatgctt ctatccaaaa ggcattacaa aataatcacc agattcacct cgctatggca
    17281 aatattgaat ccgcacaggg gcaattgcag caagtccagt taagttggtt gcctaatctc
    17341 acagggcttg caggctacac ccagtttccc gttttaggta atccggggac tacggcaatt
    17401 gcctatcctg cctacatcat taatattttc cagcaatata agcaacaaaa aagcgtgcaa
    17461 gccatgcttg aagccagtat ttacgcgcaa tattctgcca gactggtcgt gatagcccag
    17521 actgcagcca gcttttttac cttggttgct cagaatgaag ctttgcattt atacaataaa
    17581 ctgcttaagg attatcgaac ctatctcaaa ctgactcaaa gccaatatcg ctcaggatta
    17641 atttcgcttg ataatatcac tcaaatcaaa agtcatatcc aacgaattga ggcacaaatc
    17701 aaggtggttc agcataacat tgtggtgact aaaaacgcct tgcatttttt atttaatgaa
    17761 aatcctggag atgtcgagac taaaactctc tttgaaaata tcgatagtaa tcaattggta
    17821 ccagggaatt taccagccag cgttctcaga gtgagacctg atattcatga ggcagaagcg
    17881 ttgttaaaag cagcgcatgc cgatgtgggt gctataaccg cgaatctttt accagggatt
    17941 aatttgggtg cttacttggg tgagggatcc aatgtggatg gcgccattaa attagggcaa
    18001 gcttatttaa acggcccgat aattgatttg cctctatttg ctcaggttga tgtgagtaaa
    18061 gctcgatata aggcaatcta tatcaaatac attactacta ttcgtaaggc cttgcgtgat
    18121 gttgctaatg acttgtctgc ctattcaact tccagccagc aattaaataa taatttatcg
    18181 gctttaagcg atgaaaaaca gcaatgccat ttggcagaag ttcgttatcg tcacggaatt
    18241 gatgactatt taaatttaat aaaatgccaa atcctgttag acgaatttaa gttggtgata
    18301 aaccagaaca agctggaaaa actgctatcc gtagtcactt tataccagga tttaggaggt
    18361 ggttaccgtg ggcattgatc agcaaaagac cgtccgtaca ttgcgcttat ccctgacgtg
    18421 tatttttctt tttctaatta cctggtacta ccaagtccca gaaagcgcat ggactttagt
    18481 gaccatatgg tttgtcatgt atgaatattc caccgttggt ggcgtactga caaaaagctt
    18541 tttgcgcttc gcaggcacgg ctttgagtgc catctatgga atgatagttg tttatttttg
    18601 tgccaataac ccgttgatta acattatggc tttagtacct ggtttgtttc tttacgccta
    18661 ttttttcatg gggggagata aaacgtatat tggcaccatt ggggctgtaa cgcttactat
    18721 tgtattgctg aattataacg acatagatac ggctgtattg cgggtattca atgtcattat
    18781 cggtgtcatt ggttctatgt tcatgatccg gtttttttat ccccaatatg caagagataa
    18841 agttttagag attcagttga attttatcac tctacttgca aatcttttag aaagctacct
    18901 gaatccaacc cagtctttgg cagctgtcca gacaaagtat ttagattatg aacgcaagat
    18961 gcttgatggt tttactttat acaatcgtta tattggcgaa gccaaaatag aaacaaaaaa
    19021 agctccgttt ttcatccccc attctattgc tgccatgcaa cattttaggc gtctgtttcg
    19081 tttgttcagt gtgtttgttt attatttatc tactgaggaa attcgatcgg atccatgggt
    19141 atgtgatcaa ttaagtaaac ttctatgtaa tttacagtca ctgcaaagaa aattgctgaa
    19201 acaagatgag gggcccatag ggggagacat tatggctgca gagccagaag aagaaatcgt
    19261 tatagaaaca cctaatattg aaaataaaat agcaacagaa gctattgtca ataatatgca
    19321 gaaagaaatc gctttgcttg atactgaaat taaggaaata atattaattt acgatgttta
    19381 tgatatcagg ttgaatgaga tgtaaagact cattccaata tcttgggaag gggtcaatag
    19441 aaatgaggat gaaaaagaca catgagtgac aaacccaaag ctggatttcc tgtaattatt
    19501 ttattatgcg tgtttctgtt cattctgatt tatcattact cgtatttatt tccttttaca
    19561 aacaatgcat ttgttgttgc gaatgttcgt ccagtcgctg ccaatgtaaa aggttatatc
    19621 accaatattt atgttaaaaa tgaacaggaa gtaaaaaaag gccaaccatt atttaccgta
    19681 ttcaaagccc cctatgaatt ggcctacagg aaagctgtga gcgatgttca agaggcgaaa
    19741 gcccaattat tgttattcaa taaacaagtt gagaaaacga aatatctgct gcaagcacaa
    19801 aaagagctgt atgaaaaatt ccgctttgat tatgagcata atcattctgc cttgcgtgat
    19861 cacgcagttt ctaagctgac tgtcaatacg atgttaaaag aaaaaaatgc ggcactaagc
    19921 aaattaaaag cattggaaaa agagttggag gtcaaccagc aacaagtcat tgtgcaaaaa
    19981 aagaaaattg actctttaat tgcggtgatg aagaatgcca aaatcgattt ggatgaaacc
    20041 acagtttatg ccaaacaaaa tggggcagtt caggatatgt ttgttgcatt gggaacccct
    20101 atcaaaaccc gaaaaccagt ctttgccata gcggataccg ataccttgtt tatccaggct
    20161 aatttcaacg aaacggattt aagaagggta cagccagggg ataaagtctc tattttcccg
    20221 agaatgtatt ttggatctaa aatttatcat ggagtgattc tttccagaaa ctgggctgca
    20281 agccgcctgg aaacccatcg ggcaacgcaa atacaaattg ttcgcaatag cgaaagcaac
    20341 tggtttttat tgcctcaacg cctgccagta caaattcaaa taacagacta tgacccggtg
    20401 cattacccgt taagtatagg cgctagcgcc tatgtgtata tccatactca ttaaaatcat
    20461 ggaaggtgtt agcatatctg acaggaatat tcctgatccc caaaaatact aatgcgactt
    20521 ggatataaga cttggatata acctgtaccc aatgaagcta gggagatgat gaatttgata
    20581 ttgagttaat ctaaagtaga ttgcaatcct gattcatatt ggccaatagt catgagataa
    20641 tgcggattgt attgataatt aatacctcaa actcagggca acatcatacc aatttaaatt
    20701 gattatgaac cgagataatt tataaatttg atcatgtgtt tattgatccc gtacgggtga
    20761 gaatgactga aattttgcat tatataatca acgtatgatt ttgttctgac cttacaatca
    20821 aattattttc atcagctata aaatagtgct atgcttttgc ggccaaaatg gtacgaagac
    20881 tattaagtca cattagatac gttttatttt gaaaggaatg ctctatgttt ggttttttta
    20941 gtgattacaa acaatacatc actctgagaa attttgctgt cgtttataac gggcttacag
    21001 gcttggcagt attgtatagc ttgtggagta atcctgaggc tgatccctct gaatacgtca
    21061 ttgatatctc tattcacgcg ctgactgcta tcactttaat gtgtaaacaa gcccccgaat
    21121 cggttaaagc ggttgctatg gcgttaaata cgtatagggg atttgatgct ttgtttaagg
    21181 ctatcacctc gcttccttca acaattccgg gtgttgcaaa tgctattgat gtctttaatc
    21241 accggttgaa ttttaaggaa cttgaaaaac tcggcaatga agaaactgca gaaactagat
    21301 cagcagttca gcatacaatg taaaatctgg atcgtctttt ccggtagaag atggatttat
    21361 cagccatttt ctatcgggct ctcttacgcc aatttcatgc taaacagttt attggccgta
    21421 atccaccaga aaatttttca cagctaacca atcaatataa gctttctttt tatccgaggg
    21481 gttgcgcaaa agataggcag ggtgatagct tacgataaaa ggggtattgt catagtagtg
    21541 aatatgatta cgcagctgct tcagaggaag tggtttatca agtaagaatt gtccggcgaa
    21601 acgtcctaaa gcaaggatta agtgcggttt aatgagctta atttggcggg tcaggaatga
    21661 actgcattgg gaaatttcct caagcgctgg atcacggtta ttgggaggac ggcatttcaa
    21721 aacattggca atgtagacgt cattctctgt catttcaata ctacgtatca tcttgtttaa
    21781 taaaaaacca gccttcccca caaaaggttg tcccttttga tcctcgtaaa aaccaggcgc
    21841 ttcaccgata atcatcagtt ttgcttttgg gttcccgcga gaaaaaacag tttgggtgcg
    21901 tgttttatgg agggaacaac gtacacagga tgccacctca tccgctaaag tcgctaaatg
    21961 cttttcacaa gaaggagtgg acttacgcat cacccagact tcaatcccca tagtctgcag
    22021 gtagtaattg ttcaatacat ctggcataaa aaactcatga tattaataaa atccggatta
    22081 tagactagac ttaccttttg tagcttggta cttacgcacc tccaatctct ttgttaaaga
    22141 cattatttgc cttggccttg gagttttgct taagtcctat agttaatcta ttgtatcagt
    22201 cctattttaa agaaagaaac ttaaaaaaac atcatatact cactgcctgt aagaaataga
    22261 gaattgaggg ggtgaagaat attgaacaga gactggaaag aatatgaata gccaaaagga
    22321 tttgaaacaa ttagatatta agcaagtgat tgcctgggca ttctacgatt ttgccaactc
    22381 ttcctatttt gtagtgatat ttacgtttgt ctttgcaacc tatttcacaa gctacattgc
    22441 ccccaatact atcctgggaa ccgaattgtg ggggtatacg atttcggttt ctgcgttact
    22501 cattgccctg gtgagtccta tcgttggtgc aatagctgga ttttagtggg catcataaat
    22561 cctggctgtt cgtttttacc tatttgggtg ttatttcaac aggttgtttg tggtttgcct
    22621 atcctgacag ccattctatt cccttggttt taacttgtat tttgattagc aattttgcat
    22681 tggaagtagg aactgtattt tataattcct ttttggcaaa tttggccccg gaaaactatc
    22741 tggggcggat ttctggctgg gcttggggtt gtggttatct gggaggattg ctgtgcctga
    22801 ttctatcctt atttgttttt gtgaaaggtg aacttggtgg attatggttt gggacagaga
    22861 attttgccaa tattcgtgct gttactctat ttgtggcttt atggattagt ttgttcagtt
    22921 tgcctttgtt tttaatggtg caacagaaaa caggagagca tttgacagta agtactgcga
    22981 ttaggaaagg tctccaagaa ttgcggttaa caataaaaag cctgcctaaa caacgtgatc
    23041 tattcttatt tttaatagcc agaatttttt atatcgatgg gttgaattct atattggcac
    23101 taggcggtat ttatgcggct ggcaactttc acttgagtgt tagcgatatt atggtgtttg
    23161 gaatcatttt aaatattact gcaggtcttg gcgccggatt attcgcctgg tttgatgatt
    23221 ggattggatc taaaaaaact attctaattg ctttagcgtg cctgacactt actttttgtt
    23281 ttctgttaac catccactca aatgcgttat tttgggtttt tgcaccggtg tttggtattt
    23341 ttgttggtcc tgttcaggct gccagtcgta ctttcttggc acgtttggca aaacccgaag
    23401 aaatgacgcg aatgtatggt ttttactctt tttctggtaa ggcaaccagt tttttaggac
    23461 ctttcctggt tagtacaacc actgttttag ctaacagtca gcgtttgggg atggctgttc
    23521 ttattccttt ttttatcatc ggaggcggac tattattatt ggttcatgaa aatgaataag
    23581 aaaaattccg gtaaggctga ttttccctgt tttgaagttc agtatgagtt tgaaagacag
    23641 gggaaaaatt tgcctctttg tttttacaag ttatttctta tgataaactt tgttttaata
    23701 tgaaaattat tatatctata ttatttgctt tcattagtgc ctctgggcat gcgttttctt
    23761 gctatgatcc acagactatc attcaaaaac ccatacagtt tgataaaaaa cggattgcct
    23821 taacccggga atatcagctt actcattatg gcattgattc cgagtcgata gaaatcgagc
    23881 ctaaaatgat tgtattgcat tggacttgta taccaagcct cgatactact ttccgcatat
    23941 ttgatccccc cgctttaccc accagttcgc caagacgaaa tgaattgccc ggggatttaa
    24001 atgtatcaag ccatttttta gtcgatcggg atggaactat ttatcaactg atgccagaaa
    24061 catggatggc aaggcatgtg attggcttga atcattacgc tattggtatt gagaatattg
    24121 gcggagttga tagtaaagac gatttgaccg aggagcaagc caaggcaaat gcctttcttg
    24181 tgtgctatct taagaacaaa tacccgcaaa taaaatacct tattggtcat aatgagtatt
    24241 tgcagtttaa gggaacggct ttgtggttgg agaaggatcc aaattatcaa accgataaaa
    24301 cagatcctgg cccaacattt gttaaaaaag taaaacaatt agtccttgcg tcataagtaa
    24361 aagtataggc gcttatgcag gtaatgtctg ttgggatttc tgtttagctg ttaattgtta
    24421 tttttggatt gccctatagt ttttaaataa aaatcgggtt ttgggtagtg ttggtattca
    24481 aacgttgtaa acattcctgc gtttaaatga tataaattat ggcaatggtt agcccagata
    24541 ccagggttta gcgcatcaaa ctgaattttc actgtagaat gcggttggac taacaaggta
    24601 tcgcgcatgg ctccatccaa tgattgacca tcaatttcgg ttacttgaaa gacatgccca
    24661 tggaaatgca tggggtgaga catagaggat ttattttcat agatcatttc aacacgatca
    24721 ccgaaatgga ttacttttgg cgtgacatct ggccagcttt gcttgttcat ggtccaaata
    24781 taaccattca tctggccgtc aagcacatag tgcaaatgag catttatttt tttattggtt
    24841 aaagcatttt ttccttttag ttttttttcc aactcatagt agttgacacg tcccgtttta
    24901 ttagtggtat tgggagataa tatcggggta ggtgtgttat ctgttcttag aattagaccg
    24961 gtttgtttgt ttgttccttc tgccatggct ttaacaggga atgctccgcc tgcagaagga
    25021 atctcaacaa gcaaatctat acgattacca acgccaatag ggaaagaact atctacaatt
    25081 ggctcaatgt tttcaccatc aacagcaatc aaagtggctg atagtttccc ggtatcaatt
    25141 ttaaaattgg tggaggaaga cgcattaatg attcgtaacc ttaccttttt agagggttga
    25201 actgaaatca catcaggtgc tgccaatgtt tttttgttgg cgagaaacgc atcataggtc
    25261 acgtcattca aatcgttacc agaatccatt tttttcatgt ccattttagc ggttcttaac
    25321 tgatcaaata ttaactgagg atcctggtaa gtaaaatcct ctaaaaacag gatgacttct
    25381 tgtaagtctt tatgcgggtc atccctgtca taaataatca aaggggcaga aagttgtttt
    25441 tgctcttgaa atttttgatg agagtgcatc caataggttc cggcttgcaa taatctgtag
    25501 ttaaataatt tattttgtcc tggtggaatc acgatttgac tcacgtcagg tactccatcc
    25561 tgatcgcttg ggacaattaa tccatgccaa tgaagagtta ttggtgacgc acttttattt
    25621 tcaactaaaa cataaaacat atctccttta ttggcaaagt aacctttgtt gtcctttgtg
    25681 ccgtccgggt gggtaaaggt gagtttaaat tcctttgttt tttttccttt accatcgacc
    25741 tggaattcct cttcactgac ggttatgacc gtcggtttaa gtttgctatc tgttgcataa
    25801 gagaaattaa aaagggttat catgatagtt attgccatga taaaaacgaa tattttaccc
    25861 aaagtatgta ctttaatttt attgattgac attcttttcc aatgggcttt ggcgagaggg
    25921 gtaattggat aaaagtctat agtattggcc atttcgctgg tctttccttg tcgatgatat
    25981 tttattattt ttatcagcta atgaaacagt aagcaaatct ctgatttata ggttatcaga
    26041 attagtttta agcatcaggc atgcgattgc aaataattct cgtttgtatt attgttctca
    26101 tttaattaaa tgacgcgata ttttcaagtt gaaataattc gtaaaggaca agtgacttga
    26161 tgtttttaca aggaaaatga tgaaaaggat ttttttaata tcgatcattg ggatagtttt
    26221 gaattctgcg tacgcttttg agcatattgg agaatgtggg gctcatacat ggcatgaagt
    26281 ggcatccaaa tcgggttcct ggaaaatcac tttaaaaatg atcagtcaga atggccagtt
    26341 tgttgatcct tcgtgtgcag aaattattga aactggattg gctcagaata accctttgag
    26401 ctatggcctt aaattcagca aagacgctga ttttaatctg gcgtatactt taacggctgt
    26461 gagagaggaa cagggttttc aatcaaaagc ctgtgtgttt gttatcactg ctaatggtcc
    26521 agcgcaaccg gatattcagg cgcttagcta tcatggggct gaatgcacat ggcgggtaat
    26581 caaaggggtt ggggaaaatt ttagtgtcgg gtagcactat gaagcgtcct aaaaccactt
    26641 cttttttctg aaaattagaa acaatagcag agcacacagc acgatcaagc caagtgcccc
    26701 aaagtagcca tatttccagc ctaattccgg catccaataa aaattcatcc cataaatacc
    26761 ggtaattaaa cttaaaggca agaaaatggc tgcgaaagcg gttaatactc tcatggtttc
    26821 attcatgttt tgcatcaact gattgtcgat ttgtcctaat aagccattta acatatcgcg
    26881 cagagtgtct atttcattaa caataaggtg gcagtgattc gataaattat ataaagaggt
    26941 tcgacattgc tctgatataa ctaaaatatt tcggctagtg atacgcatta atatttctct
    27001 tattgctatg gtgtaacgct tcactttcat tacgccatgc tttaattcta aaacttgcga
    27061 atacgtgttt ttattggggg aacgaacttg cgcatcgaat tgatcagcca attcttcata
    27121 ggccaaatga accttggcat aatcattaat taccccttcc aggagcaaaa ataacaaaaa
    27181 acaggatgtt ttggcatagg gcaaggattt tgggcagctt tttaacatat catacaagac
    27241 tgaagaaagt tttttagaag cagtaaagca ataattgggt gtgagatgaa taattaaatt
    27301 gctaaaatca gcatcataat tgtcattcaa cttcattgag cacaagcctt gaatttgcaa
    27361 tgtgagtgtt tcatctatct ctatggttgt ggatctattg tctttttctc cacacaattg
    27421 aatcacttgc tcgggtaatc gtaatttctc tcgcaatttt ttaaagatat ttttttggtt
    27481 taaattgcta tgtatccaat aaattttatc tttattctga taatcgatat ttaaatcctc
    27541 aatcgcaatt ttttgcatac tatgattttt taaatcaaat tcaagggcga ttgaatcaaa
    27601 cgaatgcatt tcaatgctca tccttattcg atgaatttcc tcatatagtt taaggatagg
    27661 agacttaaat gatattgaca aactcttttt ttatagcctg ggttttccaa tagattgtga
    27721 gtgtctcgta gagagctagc ctcttgtttg atttttgtct tgttttaatt ctatccctat
    27781 atataattcg gattctgttt tggcaaggaa aagtaaattt aagccaaaag gaacttaaat
    27841 gagtataaaa tgaaaatcca ttaatatagg ttatttttga gtgcttaatg agccaagata
    27901 ttgaaaaaat tgaaaagatg tatcaactga ttatggaaat aaataagttg aaacttgtgt
    27961 ttcgcaatac cataaccaat cctcaacgga atgaaagtac agcggagcat tcctggtcag
    28021 cctctatgat aacccttatt ctcatgaatg aattaaaaag agagtttaaa gacgtcgatg
    28081 aattaaaaat tattaaattg gttcttatcc atgatgtcgt tgaaatttat gcgggagatg
    28141 ttctggcttt tgatctgaaa gcaaggaagg ataaggaaaa agttgaattg gaagctcttg
    28201 aaaaactaag tgctgtctat ccttcatttg ggatagaatt gggcagttta tggcatgaat
    28261 ttgaggaaag aaaaagcctt gaggcaaaaa ttgctaaagc agccgatgcg atttgtccag
    28321 ttttccagcg tctccaggca aaacaatcct atattccttt tgatatttca atggctcaac
    28381 tagagaaaac aaaataccct tattttcaat ttagtaaaac gttcagtaac ttgtttgaaa
    28441 aattaaaagc ggatttactc aaagagcaac ttattttttt atagcgccat ttgcatttgc
    28501 tttcaacaca tcctgcccga tgtcatgtta aagatccttc aagacagatt aaaagttggt
    28561 gaatcaaaac gaatcctcta tacttttaaa aactcttatt atcaagaaga taagcattat
    28621 gtatgatgat ctatggaagc cttatgcgtc taccgaacta ccctctttaa aattgggcgg
    28681 cattgtctta aatggtcagt tactttctga aacgccgatt cacttagtgt taaaggcttt
    28741 caatcgacat ggtttaattg cgggagcaac cggaacaggt aaaacgaaaa caatgcaggt
    28801 attagcagag caattatccc taaatggagt gcccagttta gtgatggaca tcaaggggga
    28861 tatttctggc ctggctatgc caggtgacgc ttctactaaa ttactgaaca gaggtcagca
    28921 gctacatata gattttactc cgcgaggttt cccggtagag ttattgacac tgactccaca
    28981 agaccccggt gttccattac gtgcgagaat tgatgatttt ggtcccctgc ttttttcgct
    29041 gatgctggat ctgaatgata ctcaggcggg agtcgtgact gtattatttg agtatgccaa
    29101 ggcgaatcaa ctgaatttgg ttaatttgga tgattttaaa tctttgctcc aatttgctca
    29161 aacggataga ggcaaccagc tgattgccgc ccaatatggc ggagtggctt ccacatccat
    29221 tgggactatt gtccgtaaaa tcatcgagct cgagtctcag ggtgggaaac agttttttgg
    29281 tgaaccttcc tttgtcattc aggatttgct gagaacggat gcaaatggtt atggtgtcat
    29341 ttccatacta agattattaa acatgcagga caaacctaaa ctattttcca gttttatgct
    29401 gaagctattg tctgatgttt atcgccaaat gccggaatta ggtgatccaa acaaaccacg
    29461 attggttctc tttatcgatg aggcgcattt aatttttaat tattccagca aagcattact
    29521 taatttaata gaaacaatag ttaagctaat ccgatccaaa ggaatcggtt tgatattttg
    29581 cactcaaacg cctaacgata tccctgagat tgttttaagc caattggggt taaaaatcca
    29641 acactcatta cgtgctttta ctgcaaaaga tcgaaaagcc ttaaagttgg tatcgcagaa
    29701 ttttccagta tccagttact ataataccga gcaactactc actactttaa gtatagggga
    29761 agctttagtg agtgccttgg atgccaaggg tcaaccgaca cctttagttc atgcaatgat
    29821 tagagcacct gaatcacgta tggggccttt aagcaatgag gagttaaagg ttttactgga
    29881 tcaatcgttt ctttttggca aatattctca aatgatagac gcgccttctg cgaaagagaa
    29941 attggctgag ctaatctctg agcaacaagt tgcaaaacaa cagaccagtc agaaagaaaa
    30001 caattcctcg gtgttgaata gtttaagtaa aaacacctta ttccgacaag tggtacgtca
    30061 agtttttagg gaactcacca gagcgatttt gtctttgttc aagagtaaaa ggaactaggc
    30121 cagttgtctt ccatattaaa tttttaactt ataccgtaca tggtcaaaat aaaagcaata
    30181 taatcgacaa ggatatacat gataaacatc acaggaacct acactgatca atatcaattg
    30241 gctatggctc aagtgtgttt tttaaaaggg cagcataagc gtatagcgac attcgattat
    30301 ttttttcgtg ctcttccttt taaaggaggc tatgcgattt ttgcgggttt ggaggattta
    30361 ctggatgttc tcgaacaact tcgctttgaa caacaagata tagagttttt acaacatcag
    30421 aatatgcatc ctgactttat tcactatttg cagcagttta agttcaatgg cacggtgtat
    30481 acgtcgctcg agggagatgt tgtatttcct acgcggccag tggttataat cgaagccaat
    30541 ttgattgaag cacaaatcat agaaacgtta ttattaaata ttcttaattt tcagacactt
    30601 attgcgacaa aagccagacg catcaggcag gttgcgggag aaaggatttt aattgatttt
    30661 ggattaagga gagcacaggg tgctggaggg tattttgcaa gccgtgcagc catagttggt
    30721 ggctttgatt cgacaagcaa tgtcagagca gggcgtgatt attcaattcc tgtatcgggt
    30781 actatggctc attcctttat ccaaagttac gatagcgaga tacaagcttt ttctgattat
    30841 gctgaagctt ggccggagaa ttgtatcttg ttagtagata cttataatac gttgcaaagt
    30901 ggtgttccta atgcaatcaa aatagctaag gaaatggaga agagaggaca tagactcaaa
    30961 ggcattcgcc tggatagtgg cgatttggca tggttagcca agagggctcg ccacatgtta
    31021 gatgaggctg gtttaaacta tgtggctatt acagcttcca atcaactcga tgaatatgtc
    31081 atcaagaatt tgttagagca ggatgcgcct attgatgctt ttggtgtggg gaccaatctc
    31141 gcgattggtg ccccggatgc agctcttgat ggggtttata aattagcgtt tataaatggt
    31201 caacccagga ttaaattatc ggatacccct gcaaagatca ctattcctta tcaaaagcaa
    31261 gtctacaggg tgttcgaaag agatgggaca tatcgaggtg cggatgttgt ctgtctcaaa
    31321 acggaaaaaa aaattgatat gatgcatcat ccttttgaaa ttctaaaatc gctttcgata
    31381 aagcactata aaaaagaagc tttactgcat aaagtgatgg aaaacggcaa acggttacta
    31441 cctaaaaaat cacttggaga aatagccagg tttagccaac aatgttttga tcgattgcct
    31501 ttggaataca agcgttttat taatcctcat atctataaag tgggtttaag taccgagctt
    31561 aaaatgaaga gagatcaact gatagctgaa cactcaagat aattttatga aaactttaat
    31621 catcctcgac gttcaaaatg atttcatgcc aggcggctca ctgggggtac cgaatggtga
    31681 tgcgattgtt cccgttatta acagcatttt gcattgtttt gatctgattg ttgcttcaca
    31741 ggattggcat ccctctaatc ataccagttt cgcctccaat catactggca ggaaaccttt
    31801 tgaaaaaatc aagctgggga atttagaaca aactttatgg ccagatcatt gtgttcaggg
    31861 tagtattgga gcgcaatttc atgcgcaatt aaatactaat cctattgaag ctatttttcg
    31921 aaaaggaaca gaccccaata tagatagcta tagtggtttt tatgacaatt tgcataaaaa
    31981 aaccacagga cttgccggtt atctgcacga aaaaggcgct agagaacttt atttttgtgg
    32041 tttgtgcgct gatatttgtg tttattttac aattaaggat gccctcagcc agggatttga
    32101 ttgctatttg attgaggatg caactcaagc gcttgtgaag gatgatttca taagaataaa
    32161 aaaagaattg ataaaacaag gcactcacat aattaatagt tcaaatttgc ttaataattt
    32221 acaataagaa ggtctgtaaa tttgtagctt tgagctcact aattggtgat ggagctcgct
    32281 gtcgaaggaa acccggcgta tttttaaggc ccgctaccat aaggtttcgt aatgatttca
    32341 agaaggtggc cattgggatc attaaaatat aatcctcgac ctccatcatt ataattcatt
    32401 tgacctaatc gggtttgtcc cggatcagcc caataatcca ttttaagttc gcaaattcgt
    32461 ttaaaaatat catcaaattc agtttctgtt actaaaaagg cataatgctg gggagtaatt
    32521 tctttatctg ttttacagaa atcaaggctc acatggttgc tcatttcaac gaccataaat
    32581 gggccatatg gaattgcttc aggcaacccc aaaatttctg taaggaagtg agccgatttg
    32641 tcccgatctt ttacccatac gatagtatga ttcaattgta tactcatcat aattccttgt
    32701 tcctgtcgat agaagaaatt atttcattac tttttatgaa agaaaagaat agccacatca
    32761 ttaaaatgca tagaatgatg tagatggttg gtaataaata ttgtgacatt ggaagtaggc
    32821 ttaatcccaa aataaccact gtaaccaatc ccatattgat gaagttcata accgcggagc
    32881 catgggcctt atcggaggta tggctcatgg caaatgttga agcatttgca aaaataaaag
    32941 atagtcccaa atatatgaat atcatgggaa aaaatagctt gataagcgct gaatgatggg
    33001 ttaacgtggc catcatcatt gccagtgcgg caaaccctga gattaaaatt cctaattgga
    33061 ttcccgcacg ttgcgagtat ttttttgcaa attgagcaga ccagattgag ccgatggtca
    33121 gacccaagga gggtataata ttggcagtgc catactctgc tgtactcatt cctagcaaat
    33181 tgatggcaat aaatggagcc gttgctgcga aaatgtagac aaagcaggat gcgccaccca
    33241 ttaatgctcc accagagatt aatggcttgt ttttaaattg acttacatat ccctggagca
    33301 gatgtttcat ttgaaacgct tcgagttgaa gatttttttg tgtttcagga agttgtctga
    33361 ccatagcaag cagaatagca ccataaacag tacctgcata aaaacaactt tcccagccat
    33421 aatgggagtt taaaatgccg ccaattgcaa cactcaaacc gggtgctatc gcaaaagcca
    33481 gcatcaaata ggaggttttt tgcgcggcta attggggctc atagtattca ttaaccaaag
    33541 taaaagtcat cttcagtcct acacctgatc ctaaggctaa tagaaatcgt cctaaaacaa
    33601 gcaacgggta catggaaatc attccggcca atacacatag aagactgctg ataatttgca
    33661 agccaatacc tatataaagt gttttttttc ttccatagcg attcgctgta gggccataaa
    33721 caagttgccc tatggcataa ccaaccaaaa aactggtgat tgtctgctgt gcggcatgtt
    33781 ctgtgatcct gaaaaatgca gtaatgctgg gcaatgccgg tgtaaatagg acagcattca
    33841 cggaagcaaa agaaattaac aacaataaag tcacgaaaga aagtttggtt tttggataca
    33901 tgatgagaat agttttaacg attaacagtt aagaacagta taattattga caaaataatt
    33961 aacaatatca taattaatta acatttatta actatattgc aataataaag ttgaaatcat
    34021 gggtaaattt gatcaaatta cttactttat tgctgttgtt gaagagcatg gttttgcagc
    34081 agccgcaaga aaatttggag tatctacagc cgctgttagc aggcagatcg cacgtcttga
    34141 agccgagcta aaagcagagc tgttggtgag aacaactaga aaattatcat taactgaaat
    34201 cggggctcgc tattatcagc aatgcaaaaa gactcttttg gagttgaatg aggcagaaat
    34261 agcgattgct agtagtcgga atgaagcggt gggcatatta aatgttacta gcagccgtta
    34321 ttttgctatg agatttcttc tcccttattt accagagttc atggagttaa atccaaaatt
    34381 gcagattaaa attgaattgg cggaaagatt tcctgattta gctcaggaaa acgttgatgt
    34441 gctttttggt gtttctatgg agggcccccc tgaactggtg cgcaagcgag tttctacaac
    34501 acggtatgtg ctttgcgcat cgccggatta tctggcaaaa aatggtattc ccaaaacacc
    34561 ttccgaactt gggaagcacc gctatatcac tcacagcatg cgaaatccgg ataatttggt
    34621 tacatttaaa aacaatgagc agatttatgt tgaacctgtg ttatggctca atgatagtcg
    34681 tgctttgtgt gagtgcgcct tacttgggat gggaattatc aaattacatg aatacatagt
    34741 ggctgaagca ttgcaagatg ggcgcctcat tgaggttttg cctgaattcc aagagtccag
    34801 tttatcggtg tacctttatt atcaggcaag ccgatattta cagcctaaga tacgaagatt
    34861 tattgatttt tttacaaaac aacaataaaa gcaagtattt tgtgtcattc tagcaggtct
    34921 gagttcaaag caaaaaagtt cggggcacgt gcttttttta gagagagagg tcaggttatg
    34981 caatgcctca ttatgtcgca aattaggtgt gtctgttaat aaatgcttca ggttgctttg
    35041 ttagaataac aacagtcaga aacattatta actagagaat ctggaaatgt tttttaaact
    35101 tgaaaaatat aattacatac tttgcacagc aattttagaa acatatggta ataaccctaa
    35161 agttctaatt ttctgattgt ataagtgtaa taaaacatga taaaatgtgc acaattttta
    35221 ctatttgttt gcatatatat taaccctatg ggattgcaat agatagtgcc actttgattt
    35281 aattaatttt tagaagttaa tatgcataaa aaatacaact actacagtct tgaaaaagaa
    35341 aaaaaaacat tctggcaaca tatactggat attctgaagg ccccatttag gctacctggt
    35401 tgggtcgttt cttttttctt ggcacgaaat attactcatg tggcattaaa tcctaataat
    35461 attccgcaac aacgtttaat acatcttact aaaacaagca atcgtccaga ggatgatatt
    35521 gttgtcatta atttcaaaaa acggcctcct cacaaatggt ttaatgacac gctgattaaa
    35581 atagccaata ccattgcggc tcttcctttt gtaacaccaa gattacgtac aagattacat
    35641 tacgataatg agaatgacat caatcatgtg aacaagttgc ttgccgagat cgatgcgctt
    35701 gttcagggca aatcaaaaca aaagtattgc aaaggcaggg tattcgattg gtcaaaaatt
    35761 catctgaagg gattggagtt cctggaccct aaaatgagag ggtatgttta tgaacaactg
    35821 catgaaaaat atggtgatgt ctcatataca acaaaacgca aacctaatat tgaatttttc
    35881 accttaaaaa ctcctgatgg atctgaattg gattcagtgc aagttacagg tgaagacgaa
    35941 gaaaaaaaac caatggggga gcgcaagttt attattacct gtattgcgcg ggatcaaaat
    36001 ttcatcaatt ggataaagga tttaaactat acggcaaaaa atctgggcgc cacagccatc
    36061 agtttcaact acagaggcgt ggattacagt cgcggtcttg tctggacaga aaataatctt
    36121 gtcgacgata ttctggctca ggtgcaaagg ctaattagcc ttggtgccga ccctaagaac
    36181 atctgccttg atggtatgtg tataggtggg gcagtagcaa caattgccgc agcaaaactt
    36241 catgagaagg gcatgaaagt caagttgaat aatgaacgct cttttacttc tctttcttct
    36301 ctagtatttg ggtttattgt acctgaattg caaacagcaa attggtggag tcctttgact
    36361 tatggtcgct tcctactggc tggtgtagtt tatgctttac tcacgccttt aatttggctg
    36421 gcaggatggc cagttgatgt gaccaaagcc tggaatagaa tacctgctca ggataaaatg
    36481 tattctgttg tgcgagataa aaacaatggc ttgtacgatg gagtaattca cgatcatttc
    36541 tgttccattg cgtcgttggt tgattcccaa attaattcta ttttatacaa attatccact
    36601 gatcagccgc ttaccgagga agaaaaacaa attttgtgtg atgatcagtt ttctcaccat
    36661 tttaaaccaa gtcaaagtgt tttaaaaaat ccaaaataca aaggcccgca ttttatttcc
    36721 cgacaagatt tggtagctga gcttggtcat cgagaggaat ataccaatca tgattatttc
    36781 cttgaccgct tgcgggaaaa atttcaattg gatcgcgcaa tacggccagt ggttctggtt
    36841 gaagaggggg aaaagaatat agatgggatt ccaccgcaac tatctaatca taaagagcgt
    36901 cccttaatta ttgccagcag tggaggaaca gggcatatct ctgctactca tggcatcatt
    36961 aatgatttac agagtaaaac aaataatgtt gtcattacgc agcatcatgc ggaactctac
    37021 aaaaacaagc cctcctcaat tacatctgtt ctgattcgca ttggtgtttg gttgacctct
    37081 cttcctatcc tggaggatat actgagaggc gttatgaggt ttatcggata tcccgtttta
    37141 ccgagttcct ccatcttttg ggatcaaatg tctaaaatac agctgggcga aactaaaaaa
    37201 gaaaatggaa ttgaaacagg cagaacccgt ccttatgtgg acatgttgtt ggatatatac
    37261 ccggagggat atgaatacac agcattcaat aacgcgactc acttgacttc cagtattgaa
    37321 gacattcaaa cgatgataag cttcaaggga catgttgaag aggataacag gaatatagtc
    37381 tatcaaaata tattacaacg ccttatgcat gcggcgaaac agaatactcc ttacacaaga
    37441 ttaatttcta cacaggcgct ttctttggga gctatttgcg atgccgttaa atactacaat
    37501 acagtatttt tgcctgtata caatgctgaa agaggaacct cctatcagcc tattaccatt
    37561 gatcagtata tgaccgattt gccttcactg ggttgtattc attttatgaa taatttggag
    37621 gagctcacat cagagcaaag gcaattgatg gagatacatg cagtaaatat gagtgaacca
    37681 tttaaagagg cgcattttgg taaagaacaa ggctttaaag cggttcataa cattgaccca
    37741 cgtaataacc caatgattcg caatgccttt aaagaccctt cattgacaaa atacctggat
    37801 aagacacagt catttgattt gcactttaat gtttataaaa aggaaaaaca aaatgcactg
    37861 ccagtactga atggtaaaga aaaaattaca atcaaaccgc atgctaaaat tgcttccatc
    37921 atgataggct ctctggcggc aaatgcttca gctgattatg cgcagtattt attgaatcag
    37981 gggtatgatc atatttttct tttcggcggg cttaatgatt ccattgccgc acgcatcgat
    38041 cagatcatca attcttaccc tgactccagg cgtgatgaga tacgtaagaa aattattctc
    38101 cttgggaatc aatctgatgt tgaaatggca ccgataatga ccagaagtaa ctgtgtggtt
    38161 atccgtgggg gtggtttaag tgtcatggag caaatggcta tgcccattat ggatgagaaa
    38221 attgtattac tacaccatga agataatgaa gagggtcctt taacgtctgg tctcagttgg
    38281 gaggatggga atgccgataa attaattgag tatctatccg aaaaaggggc atatgctaaa
    38341 aaaacatcac ctggcttgtg ttccggtcat ctcaatgagg cagaaaagga ttttgaaaag
    38401 aaatatatta cccaactaaa atcgacagga accaaggaaa aagtagattt aactattcct
    38461 cagcaaaaga agcctcccct gaaaaaagag tggggtagaa aaacaggtta tactgaactc
    38521 gggcaagtat tatcgcatca acatagattt tttgaaacga ttcctgaagt aagagagcca
    38581 ttctgttcta aggaagattt acatcataat gagctttcca gtcaaagttt ggttagtgta
    38641 acagttggtt gataaaatat gcctctaatc atcaaggctc gttttagtgt agccttctct
    38701 gaaacttgtc tgctcaggat agtatcaatt tgtgattgga atgcgtactg aacgatgcaa
    38761 cttcaaatca ttttttgtcc acagattgac cttataagga atttcagtat aataatttta
    38821 ctttttgaga aataaaagta atttattgcc attaattgca tgttttaata atagagaaat
    38881 caatatgtct aaaattttag atagcaatgc atatcgcgag atggaaaatg agctcaataa
    38941 tcttttcgag caaatttata ggcatggtaa aaatgacaga accgcgatta cattacaagc
    39001 gcagcaattt ttagataatt tgagtgaatc cataagggat cattcccgct tattgcttac
    39061 caaaattgcc agagaagaac tcatttattt taaggaaaag aaacctgggg attcgcgaga
    39121 gaaatttcaa aatatcgata agatgagtga ttactttaac aatttatctg cattgattaa
    39181 tttctcggtg atgcagcaag aagatcctac tctgcgggtt ttttattatg atatgtatat
    39241 tcaactaatg aatttttgct atcttaaagg agatttgttt ggagcgggtg caatttatac
    39301 gggtttaata gccaataacc tccctaactc tattgactgg aagaaattga gttccgagag
    39361 tcgcctgatt tttaaagatt gtgaagaaaa atttaagcga ttatttaatc tctcaacgac
    39421 atacaatgcg cacactgatt taaaaagaca attcaaaacc gccttgatac ccgcattacc
    39481 gtcacttctc acggttcaga tatatactaa ggactcttac gataactctg tcagcgattt
    39541 tgaaagaatt caggataagt tacgtcagtt ggatatagaa cataatcagt ttcttaatca
    39601 attacaaaat gaccattcaa cctggtctaa agcttattat gactatatga gtaatgttta
    39661 tctcccgcaa agcatactcg aatacacgac tctcttaaga gagtatgaag aacttgtcac
    39721 aacgcatggt ggccctattg cgaagcagtt tcataaggat aagaattcag aacctctggt
    39781 aaattttgtt gaatcgacgc tatcagaaaa tcaatcagtg ctcaaagtat ctccttttca
    39841 aaatgagaga attgttgaaa tgctttccga gattgacgtg gtaaaaagtg agtgctcaag
    39901 cccagctgtg tttgatgacc tctcctggaa gttgagagaa atgaattgta ctttggtaaa
    39961 agccaaatct attggtgatt atacttccaa aatttttgca aatcttgata ctccatacat
    40021 ggaagaaaat actaccgatg aagatccgaa accagtgctt gtagaagagc tgcaaacgga
    40081 tgatgtgtac caacttcact atagtagtat gaaattgatg cgtgagctcg aacaggctat
    40141 agaagatcat aaaccgaagg ttatttatca ttgttcaggt gaggcaaact atggtttctt
    40201 taaggaagcc agaaggaggg ggagacattt gagtccagaa ccaatggata tcgaagtcat
    40261 ggatatggat gtgatggatt atgatgagaa cgatttacgt gaccaaaccg ttatgattac
    40321 ggtgtaattt gtcttgcatc tataattgca atccatcgga tagctcttat agatgccaat
    40381 gcgattttta tcttattttg tcctatttcc ttgcagaagt ttttctatct cttctggtgg
    40441 tataggcttg ctcattaaaa agccctgaat gtcattacat cgccttttct tgaggaattt
    40501 aagctggttt tgcgtttcga ctccttcggc gatgatatta aaattcatgc tttgcgctat
    40561 agcaataatc gcatcaatga tcgcttcatc tcctggcgat tttgaaatat tctgaatgaa
    40621 tgattggtcg attttaagac tgtccacagc gacctgtttt aataaattca ggctggaatt
    40681 tcctgtgcca aaatcatcca aaataatcaa cacacccagt gatttcaact ttttgatcgt
    40741 tctttgtatt tctttatcaa taatcacatt ttcagtaatt tccagctcca gacaatgagg
    40801 ttcgactgaa tattctttta aaaggtcctc caccattgca gggaaatcgc gttgtcttaa
    40861 ctgtttactt gctacattac aggcgacacg aatgtcaggc agccccattg tgcgccacgt
    40921 accaatttgt ttacaggttt cccgcaaaac ccactctcct atcggtacga taagccctga
    40981 actttcggca gtaggaataa aatccaaggg taaaataata cctttttgcg ggtgattcca
    41041 ccggattaat gcttccatgg aacgaatctc atgagtatta actgtaaatt gaggctggta
    41101 aaggaggaaa aactcatgat ggataatcgc tttttgcaag tccaattcca actgaaattg
    41161 ctggtttgtt tttttgttga gtttctctgt ataaaatgag aattggttac cgccgcgaaa
    41221 ttttgcctga tacatcgcca tatccgcatt actgagtaaa gtgtttactg ttttgccatc
    41281 cgttggataa acgctaatac ctatgctgac atgaatattg atgtctcttt cagcaatatg
    41341 aaatggtttt tgaaaggatt tgaggatttt ttgagctact ccggcaattt gctcttcaga
    41401 attaaacgat tgaaaaagca ttacaaactc atccccacca agtctcgcga gagtatcttc
    41461 ttttcgcacc aaagatcgta atcgtttagc aacggcacac agtaattgat cgcctgcttc
    41521 atggctcaag
//
LOCUS       DASLOE010000011        40569 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.011, whole genome shotgun sequence.
ACCESSION   DASLOE010000011 DASLOE010000000
VERSION     DASLOE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 40569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40569
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.011"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            9..1985
                     /locus_tag="U6309_000449"
     CDS             9..1985
                     /locus_tag="U6309_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN5503906.1"
                     /translation="MNYRPDIDGLRAIAILLVLIYHGGLSLFPSGFIGVDVFFVISGF
                     LITTIIHESLNKNYFSFFDFYNRRLWRLQPVFVVLIVVTTLLSVLFYLPDDLIEFSRS
                     ARKASLFISNLFFNKTTTGYFSPDTHQLPLLHTWSLSIEWQCYLVLPLVMYGLYRFLN
                     KGQVIYVVHGLAILFFLLALHYSQAAPAQTYYQFSSRIFEFLIGSCIALSPRIEFSIN
                     KSILVILGSSALFFIIYTATLDNILLGYPNWHTLFVCLATGLLIALGTNFPSLAIVNA
                     LSFRPLVFIGVLSYSLYIWHWVVFSILRYQNIEERSEVLFIVYGIVFVLAYLSWRYIE
                     KPTKKFRHIPFRYTVVILLLIPVLFSHLSSLVIKSHSGYPQRFNQELVRVYKQLKSYE
                     SPYRALCIDNTRADNAWKCKIGAKEQGGRTGLMIGDSFSNHYWGFMDVLGKAAGVSIL
                     AQGTSSCISLPGIYLYDWWHFKDKIYQDCYEQTEKYYRMIQENHYDYVIIGQVWKNYV
                     SAHIINQRGDDYSAELTKKRLADALDRALGVIISSGAKPVLIESTALTHGNLHQCFFK
                     HIKLRQSYNSEECRFTLTSSDNEIWLNQLFDNMRRKYPQLIIIDPKQVQCRDNVCYSD
                     LNGIPVYRDEGHITDYASYQLGYLYLREFENPLI"
     gene            complement(2036..2302)
                     /gene="yhbY"
                     /locus_tag="U6309_000450"
     CDS             complement(2036..2302)
                     /gene="yhbY"
                     /locus_tag="U6309_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEN5503907.1"
                     /translation="MDTSFKQSLKAKAHHLKPVVLLGAKGLTEAILAETNVALLAHEL
                     IKVKINGAEKEDRMQMAEELCKQLHAELVQMIGNTLVLYRKNEE"
     gene            2405..3025
                     /gene="rlmE"
                     /locus_tag="U6309_000451"
     CDS             2405..3025
                     /gene="rlmE"
                     /locus_tag="U6309_000451"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HEN5503908.1"
                     /translation="MSRTKSSKRWLQEHFDDVYVKKAQAEGYRSRAVYKLKEIDDKES
                     LIKPGMTVVDLGAAPGGWTQYASEKMKGSGRLVALDILPMDALPNVEFILGDFREDNV
                     LQELINLIPQRTLDLLLSDMAPNMSGSSAIDIPRAMYLVELAFDFAEKMLKPGGNMLV
                     KIFHGSGFDELVKQARASFEKVVIRKPSASRSRSKETYLLAKGYNL"
     gene            3148..5058
                     /gene="ftsH"
                     /locus_tag="U6309_000452"
     CDS             3148..5058
                     /gene="ftsH"
                     /locus_tag="U6309_000452"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEN5503909.1"
                     /translation="MVKNLFLWLIIAIVLVSVFSNFGPRNSVAEKISYSQFLQEVDQG
                     MVNSVTIEDNKIIKGVTKNNKRFVTYMPMQDNALLGELLKSKVDVSGQEKQQESFLLH
                     LFINWFPMLLLIGVWIFFMRQMQGGGGRGAMSFGRSRARLLGEDQVKVTFADVAGVDE
                     AKEEVKELVDFLRDPTKFQNLGGRIPRGVLLVGSPGTGKTLLARAVAGEAKVPFFTIS
                     GSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTL
                     NQLLVEMDGFEGNEGVIVVAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQILKV
                     HLQKVPVDSHVEVKAIARGTPGFSGADLANLVNEAALFAARANKRKVGMIELDKAKDK
                     IMMGAERRSMVMDDNEKKLTAYHEAGHAIVGLSVPEHDPVYKVSIIPRGRALGVTMFL
                     PEQDRYSHSKRRLESQLCSLFGGRIAEELIFGPESVTTGASNDIMRSTEIARKMVTTW
                     GLSALGPLTFGEEEEEIFLGRSVNKHKEMSDRTAQQIDDEVRAIIDRNYQRAKEILET
                     NIDKLHLMAQSLIKYETIDTNQIQEIMSGKEPTPPEDWGSTKPMDKAESVNDAPAKPI
                     NGETIENPAEGH"
     gene            5091..5966
                     /gene="folP"
                     /locus_tag="U6309_000453"
     CDS             5091..5966
                     /gene="folP"
                     /locus_tag="U6309_000453"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEN5503910.1"
                     /translation="MNSEQFLGWLERKSQPPSSLVFEKPLIMGVLNVTSDSFYDGGKY
                     LSVDRACDQALKLIACGADLIDIGGESTKPGAPPIPLDVELSRVLPVIKQLRALADVC
                     ISIDTNKPEVMEAAINAGANVINDVYALRRDGALNVAAQLDVPVCLMHMKGEPRTMQE
                     NPSYPEGIMKELQHFFEDRIDKCQKAGLNKNKLILDPGFGFGKRVKDNLKLIYHLNEL
                     RSFGLPILLGVSRKSTIGAVLNNEVEQRLIGSITLGIYAALKGTAIIRTHDVDETNQA
                     LTMINAIYRAAKEIS"
     gene            6030..7397
                     /gene="glmM"
                     /locus_tag="U6309_000454"
     CDS             6030..7397
                     /gene="glmM"
                     /locus_tag="U6309_000454"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEN5503911.1"
                     /translation="MSQRKYFGTDGIRGHVGLSNINPEFVLKLGWAVGCVLANGARKK
                     VVIGKDTRVSGYMLESALEAGLSAAGVDVALLGPMPTPGIAYLTQTLRANAGIVISAS
                     HNLFEDNGIKFFSADGGKLPDSVELAIEAQLEKQLQTVPSAKLGKATRINDAAGRYIE
                     FCKSTIPSLSRLSNLKIVVDCANGATYHIAPNVFSELGADVVPIGIKPDGFNINQECG
                     STAPELLREKVIAVGADIGIGLDGDGDRVILVDSLGNLVDGDQIIYIIAKDRHQRGVL
                     HGGVVGTLMSNYGLELAITSLGVPFQRSKVGDRYVLETLREKDWKIGGETSGHIVCLD
                     KTTTGDGIVAALQVLSIMVKQNKALHELTAGIQLLPQTLVNLKTNNAALLASNPDVIQ
                     AVKNLEKHLNGEGRVLLRPSGTEPLLRVMVEGANASIVKQQAQMLCDDISQIDKKMTE
                     SLPST"
     gene            complement(7467..10922)
                     /gene="lepA"
                     /locus_tag="U6309_000455"
     CDS             complement(7467..10922)
                     /gene="lepA"
                     /locus_tag="U6309_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LepA"
                     /protein_id="HEN5503912.1"
                     /translation="MNEIILHLMNEISTLTPPINPLNLKQIFSSTLLPEGKAPVEVES
                     QLNLLLLIKKYVDEYSSLAEEQEEYKRKKHKLDEVMKKGEELQRKISDLAKKIEHAKN
                     ELNPLYKEIEPKANEVLSKQNPLAPPYKFPEYGEVQKSIVVGHYSYLFKTSKEIETTR
                     AKLKLVLLKNKWNALMTDLSQLGIQPPVEEDAVDSLQKFIAAVELEIKKLTRACEKWD
                     ELQKRFAKQLSATDINKKETELEKQIELLLKDLGRIEAVVTNHSLAPDLKAELTRKFQ
                     ESEDPQGLIQEYQNQIDGLLSNFNPSAWLSWYSDSNYSDNQQKLKNSVSFLQLLAQQK
                     ELKIKHHELVKQQELLIKAIPETPSNEEDISIYKKLVPDAIDLINEIPIESIPNFVLP
                     SGLSYDSSPADFYLVLLILMPKVSEKKEQFSQALEKLQEMPALGKQIQQLRSGYDLPI
                     TMDVQLPSLQEAEENKDSLNEEDPLKEELKSQIKLCQSYIETAKQIDLLMKDHGQTTN
                     EAKALIEQLRLISNQPLKNEDINSIKEDLSTLANQIQSLVSELNQLPLPILTGEKIDP
                     PLEKQEIAVDKAIPLTVELMHPAQVIQIGNDSHPEKRQLDNEEETPIVLESLRPRQVI
                     HKESESISQKLQVGNGQEMPVVLDSTCPPQIIHKELESTTEKLHVDNGHEIPVVLEST
                     HPPQIIHTESESAGKKQRVEVEQEIPVVLELTRPSSMVEKDSGVTVEKQQVTERQDTP
                     IVLESTRTTPMVQKDSGLSGEKQHKEIGQEIPAVPELTLRTQVVQQGSESSEEQQVPN
                     GQETLAVVKSSPSEPLRPSTPEVPVISRKPNGLSLFNGHDELSEDNILTFFDEAGNQI
                     SISSEEDSETLAIDRGKVNSLGIDKAFKEKPVSTGHELDKLSNPQHQESAPSISPLPS
                     SSILLKQKLDSFHVQNMEYIKQHSEEIQLWYKGLYDAAQSSCLNEALGLKALHLFKDI
                     LFELKNQNDLSVLLAYKRMCPNPLQDIQNILRLKPALPILDESIDEEQQLKNWPEELQ
                     KFHQQYVKLKKEHPLEAELFIQATHSLISIKHLMELPDAKTSSREAMPLITQDPRYEP
                     LKRHRGFIKAWEYIEDFFRMLIGKLTGQDEYEYSKRPCFFKTRSHRLLEEADTMLQSM
                     APTSS"
     gene            11166..11915
                     /locus_tag="U6309_000456"
     CDS             11166..11915
                     /locus_tag="U6309_000456"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEN5503913.1"
                     /translation="MRQKIVAGNWKMNGQIQQVTELVSQIEELIGFDCAAQVAVMPPS
                     IYIPKVRDCLRTGKIVVGAQNVYPKDYGAYTGELSAPMLKDFDCRYVLVGHSERRQFF
                     HEDENFVAQKFHHVKDHGMIPILCVGETLSERENGKTEQVIAQQVLAVSAKGKDCFRD
                     CVVAYEPVWAIGTGKTATPEQAQKIHQFIRDLVGEINDSDAKHLTLIYGGSVNENNAK
                     ALFSMPDIDGGLVGGASLNAKQFVEIVKCIN"
     gene            11903..12226
                     /gene="secG"
                     /locus_tag="U6309_000457"
     CDS             11903..12226
                     /gene="secG"
                     /locus_tag="U6309_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEN5503914.1"
                     /translation="MYQLILMIHVLIAVILIGLVLIQHGKGADIGAAFGSGASNTLFG
                     SQGTGGFLFKLTGGLAMTFFITSLMLSYMVSTQNQKAGQLAIPQQTGAPVSSVPVPVE
                     KNNKK"
     gene            12297..12381
                     /locus_tag="U6309_000458"
     tRNA            12297..12381
                     /locus_tag="U6309_000458"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12331..12333,aa:Leu,seq:gag)
     gene            12406..12762
                     /locus_tag="U6309_000459"
     CDS             12406..12762
                     /locus_tag="U6309_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEN5503915.1"
                     /translation="MLSQYLPVLVFIIVALILGAVMIGAGSLLSKHSPNSAKNSPYEC
                     GFGAFESSHIPFDVRFYLVAILFIIFDLETAFFFPWALALRKIGWFGFYAMMLFLGLL
                     VIGFIYEWKRGALEWE"
     gene            13009..13485
                     /locus_tag="U6309_000460"
     CDS             13009..13485
                     /locus_tag="U6309_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEN5503916.1"
                     /translation="MAVAELEKKGFELTTVEKLVGWARSGSMWPMTFGLACCAVEMMH
                     VGAARYDLDRFGIIFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMPEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIRRKPVLEA"
     gene            13508..14191
                     /locus_tag="U6309_000461"
     CDS             13508..14191
                     /locus_tag="U6309_000461"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEN5503917.1"
                     /translation="MTKNEYLIEKLQANLANHITELTSAYGEVTIECEVHNLLPVMIE
                     LRDREEFSFDQLIDLCGVDYLHYGDYDWETESATEHGFSRGVERQEAKAYAVNKPRFA
                     VVYHLLSTKKNHRLRVKLFVEESHLIVPSVHHLWKSANWFEREAYDLYGILFDGHPDL
                     RRLLTDYGFIGHPFRKDFPLSGEVEMRYDAKLQKVIYAPVDIVPRIVVPKVIRNDNRY
                     IGNEGSKND"
     gene            14169..15437
                     /locus_tag="U6309_000462"
     CDS             14169..15437
                     /locus_tag="U6309_000462"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEN5503918.1"
                     /translation="MKVAKMIELKNYTLNFGPQHPAAHGVLRLVLELEGETIVRADPH
                     IGLLHRATEKLAETKPYIQSIGYMDRLDYVSMMCNEHAYVMAIEKLLGIEPPLRAKYI
                     RTMFDEVTRILNHLLWLGATALDIGAMTVFLYCFREREDLFDCYEAVSGARMHATYYR
                     PGGVARDLPDTMPQYKPSRWHNEREIEKKNHNRQGSLLDFLWAFTERFPRCVDEYETL
                     LTDNRIWKQRTVDIGIVSPENALQWGFTGPMLRGSGIAWDLRKKQSYAAYDRVDFDIP
                     VGKTGDCYDRYLVRVEELRQSNRIIRQCIEWLRKHPGPVKVDDYKVTPPRRVVMKHDM
                     EALIHHFKLFTEGFCLPRGEVYSSVEAPKGEFGIYMVSDGANKPYRLKIRAPGFAHLS
                     SFDDMVRGHMLADGVAILASQDIVFGEIDR"
     gene            15498..16001
                     /gene="nuoE"
                     /locus_tag="U6309_000463"
     CDS             15498..16001
                     /gene="nuoE"
                     /locus_tag="U6309_000463"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEN5503919.1"
                     /translation="MSANSAKILNQLMPANRMRDIDHWISKYPKDQKQSAVMSALRIV
                     QEEHGHLTTELMNAVAEYLDMPPIAVYEVASFYTMYEHKPVGRHLINVCTNISCMLRD
                     SAGVVEHLEKKLGVNLGGTTEDGRFTLRSVECLGACVNAPMMQVDKDYHENLTAESID
                     KVLEQYQ"
     gene            16020..17297
                     /gene="nuoF"
                     /locus_tag="U6309_000464"
     CDS             16020..17297
                     /gene="nuoF"
                     /locus_tag="U6309_000464"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEN5503920.1"
                     /translation="MVELNQVCYRTFHLPEPWSLSTYESIGGYSAWRKILKEQTSPQL
                     IIEELKTSALRGRGGAGFPTGLKWSFMNRNSPVQKYVVCNSDEGEPGTCKDREILTLN
                     PHQLIEGMAIAGYVMGATVGYNYIRGEFWLPFERTEAAIKEAYDAGLLGKNILGSGVD
                     FDLYNHLGAGAYICGEETALLNSIEGKKGMPRFKPPFPANYGLYGKPTTINNTETFAS
                     VPVIMEKGGEWFLKLGKPNNGGAKCFSVSGHVNKPGNYEIPLGTPFKTLLDLAGGVIK
                     GRKIKAVIPGGSSMPVLPGDVMLGLDMDYDSIQKAGSGLGSGAVIIMDETTCMVDALY
                     RISEFYMDESCGQCTPCREGTGWLVRLLHRIINGHGEPGDVEKLVNVANNIEGRTICA
                     LGEAAAWPVQSFVKHFYDEFEYFIKHKRSIVAA"
     gene            17314..19665
                     /locus_tag="U6309_000465"
     CDS             17314..19665
                     /locus_tag="U6309_000465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEN5503921.1"
                     /translation="MATIEIDGKTFEAENGKMIIEVADEAGIYIPRFCYHKKLSVAAN
                     CRMCLVEVENGRKPVPACATPITNGMKVFTKSEQAIHSQKVVMEFLLINHPLDCPICD
                     QGGECELQDISMGFGADKSEYTESKRAVDDENLGTLISTEMTRCIHCTRCVRFGEEIA
                     GVRELGATGRGEKTQIGTYVEHSMTSEVSGNIIDLCPVGALTSKPYRFKARAWELTQH
                     DSVAPHDCLGSNVHIHTRNNKLMRVVPRENESINETWLSDRDRFSYLGLNATSRASKP
                     QIKKNGQWEAVDWETALKFTAEGISRVIKQHGPEQMAAFASSSSTLEEMYLLQKLMRE
                     LGVQNLDHRLQQIDFRDQAFLPTTPVSTLPYAEIDQQHSILLIGCNIHREVPLAGTRV
                     RKAFRNGAKIYALNPVDFDYHFDLSGRVIISPLEMPMQLAKLALALTSELASLPEEVQ
                     KLLIGLEVDKQTKQIAQSLKEEKACLITGAIVENHPEASLLRTLVAIVQKLSGAKLVR
                     LTTGANSAGACIAGMLPHRTVAGKSIAEPGFNVQEALNSKLKGYLLMGVEPGYDFANP
                     AGARQSMLAAEFVVLLSAYEHESMHDYADVILPIAPYAETSGTYINIDNTWQTVKGAM
                     LPLGESRPAWKVLRVLGNLLHCKKFDYTSTEDILEEVKEAVSMTMEHEYEPYYPESLP
                     VINQSLVRVGEWPLYRIDAITRNAKELQLCAASESACIRIHPSTADRLKLEEIATVSQ
                     GDIEITLPLKRDERIAVDVVWVANAMPETVDLGHSFAAITIKR"
     gene            19678..20700
                     /gene="nuoH"
                     /locus_tag="U6309_000466"
     CDS             19678..20700
                     /gene="nuoH"
                     /locus_tag="U6309_000466"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEN5503922.1"
                     /translation="MLHTIGILIWIIIKILVIVVPLLLSVAYLTYAERKVIGYIQVRI
                     GPNRVGLKGLLQPFADLLKLITKEIIVPTRSNKYLFVIAPLFALVPSLVGWAVIPFQE
                     GVVLANINAGVLYLFAMSSLGVYGVLIAGWASNSKYAMFGALRSTAQTVSYEIAMGFA
                     LVGVLLAAGSMNLTDIVNSQKGGMLHWWFIPLLPLFLVFWIAGIAETNRAPFDLAEGE
                     SEIVAGFHVEYSGIGFALFFLSEYASMILISTFMAILFMGGWLSPFEGITFLDQIFFV
                     VPGFVWLLLKISFFLFVYLWVRATFPRYRYDQLMRLGWKVLIPVTIVWLIVTSLMVVA
                     HVKPWF"
     gene            20719..21219
                     /gene="nuoI"
                     /locus_tag="U6309_000467"
     CDS             20719..21219
                     /gene="nuoI"
                     /locus_tag="U6309_000467"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEN5503923.1"
                     /translation="MKKVYHYIIHYVRTYLLLELLAGLWLTVKYFFRKKITVQFPEEQ
                     TPLSPRFRGLLALRRYPNGEERCIACKLCEAVCPALAITIESEQREDGSRRTTRYDID
                     MFKCINCGLCEESCPVDSIVVTPIHHYHISERGQNIMTKEKLLAVGDLMETQLAADRA
                     ADEKYR"
     gene            21231..21890
                     /locus_tag="U6309_000468"
     CDS             21231..21890
                     /locus_tag="U6309_000468"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEN5503924.1"
                     /translation="MHEWVIQAIFYIFALLTVLSALMVISQNNPVRCVLFLVLTFFTS
                     AVLWVLAEAEFLALILILVYVGAVMTLFLFVVMMLNIDIESMKSHLIKYLPFGLIIVA
                     LLTGLLMVAIPKDLFKNSVQIQEKPLSVSSQLMDLSTQDELVPPENATSNTEKLGMVL
                     YTDYILAFEIAAVILLVAIVSAITLVHRGAIRSKRQDITQQIMTRRAERVKLISMKSE
                     K"
     gene            21908..22213
                     /gene="nuoK"
                     /locus_tag="U6309_000469"
     CDS             21908..22213
                     /gene="nuoK"
                     /locus_tag="U6309_000469"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEN5503925.1"
                     /translation="MIPVYDYLVLGVILFGLSLVGIMLNRKNIILLLVCVELMLLAVN
                     TNFIAFSHYYNEVGGQVFVFFILTVAAAEAAIGLAIVMLLYRNRGNIDVDKMNHLKG"
     gene            22218..24191
                     /gene="nuoL"
                     /locus_tag="U6309_000470"
     CDS             22218..24191
                     /gene="nuoL"
                     /locus_tag="U6309_000470"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEN5503926.1"
                     /translation="MSIQQVCLVIVLTPLVGSAIAGFFRNQIGRVGAHTVTILGVAIS
                     LVCSIFLAWGLFSGSIPNTNSLVYHWASGGALIPYEFNIGFLIDPLSVVMLVIVNFVS
                     LLVHVYSIGYMADDDGYQRFFSYISLFTFMMLMLVSANNFLQLFFGWEGVGLVSYLLI
                     GFWYQKESAIEGSLKAFLVNRVGDFGFVLGIGLIFAYAGSLDYEKVFTSANYLASQNI
                     ELFSGYSWSLITVICLLLFVGAMGKSAQVPLHVWLPESMEGPTPISALIHAATMVTAG
                     VFMIARISPLIELSTAALSTVLVIGATGALFTGILALVMNDIKRVVAYSTLSQLGYMM
                     VAMGASAYSTGMFHLLTHACFKALLFLGAGSVIIGMHHEQDMRKMGGLWNKMPITYVT
                     YVIGSLALCAFPPFAGFYSKDTIIEAAQLSQIPGSSYAYFCVTAGAMVTALYTFRSLF
                     MTFHGKPRMDEHTLSHLHESPWVVWLPLVLLAIPSIILGYVLYMPILFDTPTLLGSSI
                     FVLPEHNVLAELAHEVSSPFASAVHAIYSLPFWITVLGAVIAWVCYIAVPSIPGYLAR
                     YFSIIYSILLNKYGFDRFNELFFVKGARGLGIAFYKIGDQKLIDGAVVNGSGRLVRWF
                     SGKGRKIQSGYIYHYATVMVFGLLAFLCWLILD"
     gene            24207..25712
                     /locus_tag="U6309_000471"
     CDS             24207..25712
                     /locus_tag="U6309_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEN5503927.1"
                     /translation="MHYLLNLLIWLPILGGVFVLLTGDDKNPNVSRYLTLFTILLCLV
                     LCIPLVKGFDLQSFGMQFIEEIPWMPALGINYTLGIDGLSLLLIVLSIFTNLIVILAT
                     WDSISKKVSQYLSAFLIMQGLLVGIFSAMDAIVFYIFWEATLIPMYLIIGIWGSDNRV
                     YAAIKFFLYTFLGSVLMLASFLYMGYIAGSFKIETFYAIKLGMTAQTLIFIAFFLGFA
                     IKIPMFPVHTWLPDAHTEAPAGGSVVLAAILLKLGAYGFIRFSLPIVPDACSKYAPLM
                     VALSLIAVVYIGLIAIIQKDMKKLIAYSSISHMGFVTLGCFAIFAIARHGGLSNAGLI
                     LEGAIVVMISHAFVSSAMFAGVGFIYDRMHTRQLADFGGIVNTMPIFASFFMLFAMAN
                     AGLPGTSGFVGEFMVILGSIKAGFWVAFWAATTLIIGAAYTLWMYKRVIFGPVANKKV
                     AALKDISGFELSAYVLLAIMVIALGVYPKPMLEFVHQTVSHTLALADKSKL"
     gene            25766..27208
                     /gene="nuoN"
                     /locus_tag="U6309_000472"
     CDS             25766..27208
                     /gene="nuoN"
                     /locus_tag="U6309_000472"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEN5503928.1"
                     /translation="MMTLLENIHVAIPEMIILITACLALLSDLFFRHKLKSIAFYISC
                     AGIIVSAAVSFVFIGSYKLLIFNKLFISDDISHLMKLFIDITVLLSFVYSQNYLDERQ
                     MPTGDYYVLGLFSTLGMMILVSAHSLLSLYLGLELMSLPLYAMTAIRRTDSDASEAAM
                     KYFVMGAIASGMLLYGISLVYGATGKLDLLDVANTVAVNWQQQNTLFAFAMVFILAGV
                     GFKLAAVPFHMWAPDVYQGAPSSVTLFISTAPKIAALGMALRFLTIGLVDITMQWQQI
                     ILVMALLSTGIGNLLAVIQTRIKRLLAYSAISHIGYALFGILAASSAGYAAALYYILV
                     YAVMSAAGFGLVVLMSNHGMEIDSIDDLKGLNKRNPWLAFMMLLVMFSMAGVPPTVGF
                     FTKLLVLKALVDVHMTWLAVVGLLFTVIGAYYYLRIVKIMYFDQPENEDIVRIGTGNT
                     IVYSLNCLSLLYLGIFPGALIAACINAFAN"
     gene            27274..27350
                     /locus_tag="U6309_000473"
     tRNA            27274..27350
                     /locus_tag="U6309_000473"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27308..27310,aa:Met,seq:cat)
     gene            27464..27907
                     /gene="rimP"
                     /locus_tag="U6309_000474"
     CDS             27464..27907
                     /gene="rimP"
                     /locus_tag="U6309_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041173832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEN5503929.1"
                     /translation="MINDDLIVLLEPIIKNMGYELWGCEYLSQGKHSLLRIYIDKPDG
                     IGIDDCQEVSKQVSAMLDVEDPIPGHYSLEISSPGIPRPLFSIWQYQRYLGYEIHVKT
                     FKPVNGKRKLSGIIVSASEDTIVLDINNEHQEILLSNIVKANLTV"
     gene            27919..29397
                     /gene="nusA"
                     /locus_tag="U6309_000475"
     CDS             27919..29397
                     /gene="nusA"
                     /locus_tag="U6309_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEN5503930.1"
                     /translation="MSKELLLVAEALSNERGVSKDVIILAIQAALESATRKILGLDIG
                     VRIKIDSKTGDYETFRYWDVVNEEELEFPDRQLSLEQAKERKPSAAVGDRIEEIIPSI
                     EFGRIEAQTARQVIMQKVREAERELIIDQFRSKLGQLIYGTVKKVTRDNVIIDLGGKA
                     EAFLPRSEMLPHEMFRPNDRVRAYLYEITPQARGPQLFVSRTRNEMLIELFRIEVPEI
                     GENIIDIKAAARDPGNRAKIAVKTNDGRIDPIGACVGMRGARVQAVSSELGGERVDII
                     LWDDNPAQLVINAMAPADITSIVVDEDTHTMDLAVAKEQLSQAIGRNGQNVRLASQLT
                     GWTLNVMTTEEFESKNQEESSKIVNLFTNALEIDEEIATLLVAHGFSSLEEVAYVPKE
                     ELLAIEEFDEEIVEELRNRANDKLLEMALSSGKGLSGTPDDSLINMEGMTEDLANKLA
                     SKGITTMEDLAEQSVDELMEIEGISEEKAGALIMKAREPWFQ"
     gene            29486..32092
                     /gene="infB"
                     /locus_tag="U6309_000476"
     CDS             29486..32092
                     /gene="infB"
                     /locus_tag="U6309_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEN5503931.1"
                     /translation="MADVTVKQLAQVVGIPVERLLNQLQEAGLSFTDDQQTVNEEQKR
                     ILLNHLKGSSNRDISAAPERITLRRKSMSQVTVGHDMHSGKTVNIEVRKKKTFIKRSA
                     IPEQAEIEEPVVPPVVEEPVHEEITVVSDVSAETPDLKETEEHPVIEPVAELDETVKE
                     EEKISLEENTAESQDELTHANTDVIENLVDVVEETIPVSKKEEVKPEKVSKKKHLEQT
                     DSDISEFKKGKKKPKYHTFEHDEEEQELHRRGGRSKFKKKKGTEKSDKYREAEETLTH
                     GFALPTAPIVREVLIPETITVAELAKRMSVKAAEVIKVMMSLGAMATINQVIDQETSV
                     IVVEEMGHKPVIIKEDAVETGLGEAISKGTKTEGRAPVVTIMGHVDHGKTSLLDYIRR
                     TKVAAGEAGGITQHIGAYHVSTPKGNITFLDTPGHAAFTAMRARGAQATDIVILIVAA
                     DDGVKPQTIEAIQHAKAAKVPIIVAINKMDKPDADPERVMNELSVQEVIPEAWGGDTM
                     FVNISAKSGMGIDDLLDAILLQSEVLELKAVTDGAAKGVVIESRLDKGRGPVATVLVQ
                     SGTLHKGDILLAGFQYGRVRALVSDNGDLVDSAGPSIPVEVLGLSAIPHAGDEAVVVP
                     DEKKAREVALFRQGRFRDVKLARRQKTTIEGIMENMTATESKVLNIVLKADVQGSLEA
                     ISDALTKLSTDEVKVEVISSGVGGITESDVHLAIASNAILIGFNVRADGTAKRLAEQE
                     SVSIHYYSVIYDIVDQIKGALTGMLAPQFKEEIIGIAEVRDVFKSPKIGAIAGCMVIE
                     GVVKRNNPIRVLRSNVVIYEGTLESLRRFKDDVLEVRQGFECGIGVKNYNDVKPGDLI
                     EVFETVEIKRDL"
     gene            32096..32467
                     /gene="rbfA"
                     /locus_tag="U6309_000477"
     CDS             32096..32467
                     /gene="rbfA"
                     /locus_tag="U6309_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEN5503932.1"
                     /translation="MSNNFKRTDRIAEMIQRKLALIIPQEIKDPRLKGFVTISAVKVA
                     ADLGHAKIYFTVLNEDKSVVTNILNGAASYLRSALARSITLRTVPQLHFIYDESIEYG
                     QRLSRLIDEVNPPDSNSDDNN"
     gene            32454..33365
                     /gene="truB"
                     /locus_tag="U6309_000478"
     CDS             32454..33365
                     /gene="truB"
                     /locus_tag="U6309_000478"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEN5503933.1"
                     /translation="MTTIESQCSIDGILLLNKPQGMTSNAALQKAKHLFGAKKAGHTG
                     SLDPLATGMLPLCFGEATKICQYLLNADKSYETIGRLGSKTNTADCTGEVIFCIENYT
                     VSHEEMIATLEKYKGKIKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLKL
                     EQFDGECFTLTVSCSKGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENSRMYTLDE
                     LQNMSLLQRLACLIPIDQAIQHLTPVILSGSEVIAIRQGKVISNKTDAGEGEDLRLYG
                     EQSQFIGIGQALIHGDIKAKRLVSFAL"
     gene            33513..33788
                     /gene="rpsO"
                     /locus_tag="U6309_000479"
     CDS             33513..33788
                     /gene="rpsO"
                     /locus_tag="U6309_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEN5503934.1"
                     /translation="MSLNSAEKAEIINQYKRGDKDTGSPEVQVSLITGRIKYLTDHFK
                     ENKKDFHSRRGLQELVNKRRKLLKYLKRNDQDRYQTLIQNLGLRDSY"
     gene            33903..36092
                     /gene="pnp"
                     /locus_tag="U6309_000480"
     CDS             33903..36092
                     /gene="pnp"
                     /locus_tag="U6309_000480"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEN5503935.1"
                     /translation="MAKITKEIVFGNHKLILETGEVARQADGAVMASMNGTQVLVTVV
                     WKKDGGESNDFFPLTVNYQEKFYAIGKIPGGFNKREGRPSDNETLISRLIDRPIRPLF
                     PDNFFNEVQIIATVLSLNPEVSPDIIAMIGASAALSISGVPFNGPIGAARVGYKDGVY
                     LLNPSRKEQEESKLDLVIAGTKDAILMVESEAQELSEDIMRGAMLYGHEMMKSVIKSI
                     EELAREVGKSKPEWKAPEIDTVLKARINDVARNEVEAAYLIKDKQQRYQRLDELREQT
                     ISALLAENDELNADIIANMFGELERSIVRNRILDGEPRIDGRDHRTVRPISIRTKFLE
                     RTHGSCLFTRGETQAIVVATLGNERDAQILDGISGETRDRFMLHYNFPPYSVGETGQV
                     GSPKRREIGHGRLAKRALMAVLPDTNEFPYVLRIVSEITESNGSSSMATVCGTSLALM
                     DAGVPLKAPVAGVAMGLIKEGDRYAVLTDILGDEDHLGDMDFKVAGTEKGITALQMDI
                     KISGITNEIMEQALEQALEGRTHILGVMNNALAEHRTELSQHAPRITTMKVAEDKIRT
                     IIGKGGATIKGLIESTGVSIDIDDSGVIQLFSPDKMALEEAQKQIKALIAEIEVGQTY
                     QGKVSKIVDFGAFINLLPGKDGLLHISQICADRTQKVEEVLQEGQEIEVFVAGIDKQG
                     RVKLEWKDKPQAEAKEVEDAPVSATFLTMEEQSEEINSGNKISEEEE"
     gene            36235..37317
                     /locus_tag="U6309_000481"
     CDS             36235..37317
                     /locus_tag="U6309_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEN5503936.1"
                     /translation="MLFFLVLISSASLGQDLPLHLLKYPSGFSVSIYAASVSDAREMA
                     LGSKDIVFVGSRNAGKVYAVIPDKKNSHGTRVLTIASGLNMPNGVAFHKGALYVAEIG
                     RILRFDNIESNLNNPPEPVVVTTKLPKEKAHGWRFISFGPDDKLYIGIGAPCNVCLME
                     DTRYATIMRMNADGQDLEIYAKGIRNTVGFDWDPFHHNLWFTDNGRDWLGDDLPPDEL
                     NVAPRKGMDFGFPYCYGKSTADPVYGKIHSCSEFTPAVFEFPAHVAPLGMSFYTGKLF
                     PSNYWGQIFISEHGSWNRSHKIGYQITIVKIKNNHVTEVKPFISGWLQGEKAWGRPVD
                     TLVMPDGSLLISDDYANVIYRVGYQQ"
     gene            complement(37377..37916)
                     /gene="folE"
                     /locus_tag="U6309_000482"
     CDS             complement(37377..37916)
                     /gene="folE"
                     /locus_tag="U6309_000482"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEN5503937.1"
                     /translation="MEKLYSNLLKELGEDINREGLKDTPGRAANALRYLTKGYNENLD
                     EIINGALFDSDMSEMVIVKDIELYSLCEHHLLPFLGKCHVGYLPDGKVIGLSKIARIV
                     DFYARRLQIQERLTGEIAHCIEATTGARGVAVVIEAKHLCMMMRGVEKQNSVMTTSVM
                     LGEMRNNSSCRLEFLNLIR"
     gene            complement(37928..38269)
                     /locus_tag="U6309_000483"
     CDS             complement(37928..38269)
                     /locus_tag="U6309_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEN5503938.1"
                     /translation="MNCLFCKIAQGEIPATVVFEDKNILAFRDIRPQAPTHLLIIPKK
                     HIATINDVNDDDSELLANILIRAKKLAQAEGLSEMGYRLVFNVNSGGGQEVYHIHLHL
                     LGGRQMTWPPG"
     gene            38399..38935
                     /gene="ppa"
                     /locus_tag="U6309_000484"
     CDS             38399..38935
                     /gene="ppa"
                     /locus_tag="U6309_000484"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEN5503939.1"
                     /translation="MSLMEIQSGRDVPNEVNVIIEIPMHGEPVKYEVDKKTGALFVDR
                     FMTTAMFYPTNYGYIPNTLSEDGDPVDVLVITPVPLISGAVISCRAVGMLKMTDESGV
                     DAKILAVPTTKLSKMYQSMQTYQDIPQHLLLSIEHFFKHYKDLEEGKWVKVEGWVGPD
                     AAREEITSSINRYNHTKK"
     gene            39085..40350
                     /locus_tag="U6309_000485"
     CDS             39085..40350
                     /locus_tag="U6309_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN5503940.1"
                     /translation="MQLPLSTLLVLLFFLICLSAFFSGSEIGMMSINRYKLRHLVKSN
                     DKQAIRVNSMLARPDKLLSVVLIGNTLANIVASTLATLIGQRFYGDAGVAIATALLTM
                     LILVFAEMTPKTLAAIYPQQVAFASSLPLSILQWIFSPLVHLISLISNGILRLFNISV
                     KRMQKEMLTGEELRSVVHEAGGLMPVEHKSMLISLLDLEQATVEDIMVPKADIVGIDL
                     DLPWIELLDQLETAQHTRLPIYHGSIDHLVGMIHVRNILNLALENKLDMENLLNSAEV
                     PYFIPEATPLNIQILNFRKMKRRSCFVVDEYGDILGLVTMEDILEEIVGEFTTDIADL
                     SRDITPQQDGSVIVDASITIRHLNRMMGWSLPLIGPRTLSGLIIEHLGYIPPPDSCLV
                     IDHYHIEILRVSDNMIRGVKMIKANKKRK"
BASE COUNT        11897 a         6941 c         8978 g        12753 t
ORIGIN      
        1 taagtggcat gaattataga cccgatatag atggattaag ggccatcgca attttattgg
       61 tcttaattta tcatgggggc ctctcgcttt ttccatcagg ttttatcggt gttgatgtat
      121 tttttgttat atcaggtttt ctcattacta ctattatcca tgaatcactg aataagaatt
      181 atttttcatt cttcgatttt tataatcgaa ggttatggcg attacagcct gttttcgttg
      241 ttctaatcgt ggtgacaacc ctactaagcg tattgtttta tttgcctgat gatttaattg
      301 aatttagtcg cagtgcgcgt aaagcatcac tgtttatttc caacttgttt tttaataaaa
      361 caaccaccgg gtatttttca cccgataccc atcaacttcc tttgctgcat acttggtctc
      421 tttcaattga gtggcaatgt tatctagttt tgccactggt gatgtatggc ttatatcggt
      481 ttctaaataa agggcaggtt atctatgttg tgcatgggtt ggccatttta ttttttttgc
      541 tggctttgca ctactcacag gcggctcctg cccaaaccta ttatcaattt tccagtcgta
      601 tttttgagtt tttaatcggt tcttgcatcg ctttaagccc tcgaattgag ttctcaatca
      661 ataagtctat tttagttatt ttgggttcat cagcattatt ttttataatt tataccgcta
      721 cactggataa tattcttctt ggatatccta attggcatac actttttgtg tgtctggcaa
      781 cgggtttgtt gatagcatta ggtacaaatt ttccttcact tgctattgta aatgctctct
      841 cttttcgacc attagtgttt ataggagttt tatcttattc tctttatatt tggcactggg
      901 tcgtcttttc aattttaagg taccaaaata ttgaggagcg gtctgaggtt ttatttatag
      961 tttatggcat tgtttttgtt ttagcttatt tatcctggcg atatatagaa aaaccgacga
     1021 aaaaattcag acatattcct tttcgttata cggttgtgat tttattgctg atccctgttt
     1081 tattttctca tttaagctct ttagttatta aatctcattc tggatatcca caacgattta
     1141 atcaggagct ggttagggtt tataaacaat taaagagtta cgaaagccca tacagagcgt
     1201 tatgtataga taataccagg gcagacaatg cgtggaaatg caaaatagga gcaaaagagc
     1261 aaggtggcag gactggcctt atgataggtg attctttttc caatcattat tgggggttta
     1321 tggatgtgtt agggaaagct gctggtgttt caatattggc tcaaggaacc tcttcttgta
     1381 tttccttacc aggcatatat ctttatgatt ggtggcattt caaagataaa atatatcagg
     1441 attgttatga gcaaacggaa aaatattatc gcatgattca agagaatcat tacgattatg
     1501 tgattatagg ccaagtatgg aaaaattatg tttcagccca tattatcaat caacgtggcg
     1561 atgattattc tgcagaatta accaaaaagc gtttggcaga tgccttggac agagcacttg
     1621 gcgtgataat atcttctgga gcaaaacctg tattaatcga atcaaccgcg ttaacgcatg
     1681 gaaacttaca tcaatgcttt tttaagcata ttaaattaag acaaagttat aactcagaag
     1741 agtgccgctt tactttaacc agttcagata atgagatttg gctaaaccaa ttatttgata
     1801 acatgcgaag aaaataccct caattgataa taatcgatcc taaacaagtt caatgtcgcg
     1861 ataatgtttg ctattccgat ttgaacggga ttccagtata tcgcgatgaa gggcatatta
     1921 ctgattacgc ttcataccaa ttgggttatt tatatttgcg ggagtttgaa aatcctttga
     1981 tttaaaaagg ttggtttgct ttgattcccc agactacaaa acgattttac aaattttatt
     2041 cttcattttt acgatacaga accagtgtat ttcctatcat ctgaactaat tcagcatgca
     2101 attgtttgca aagctcctcg gccatttgca ttctgtcttc cttttcagcc ccattaattt
     2161 tgactttaat taattcatgt gctaacaagg ctacattcgt ttctgccaat atagcttcag
     2221 ttaagccttt ggcgccaagg agcacaacgg gctttaaatg atgagccttg gctttaagtg
     2281 attgtttaaa agacgtatcc actgcgatat cccagataat aaaatggtaa attattgcac
     2341 gttttgctgg gaaaaggtag taaaattctg tatcatagga aaaattgttt tatctttatt
     2401 cgttatgagt cgtactaaaa gcagtaaacg ttggttgcaa gaacattttg atgacgttta
     2461 tgtaaaaaaa gcgcaggctg aaggatatcg aagtcgtgcc gtatataaac ttaaagaaat
     2521 agatgacaaa gagtcgctca ttaaaccagg catgacagtg gttgatttgg gagctgctcc
     2581 tggcggctgg acccaatacg cttcagaaaa gatgaaagga agcggtcgtc ttgttgcact
     2641 agatatttta cccatggatg ctttgcctaa tgtggagttt atccttgggg attttcgtga
     2701 agataatgtc ttgcaagaat taattaattt gattcctcaa aggactttag acttgttatt
     2761 atcggatatg gccccaaata tgagtggaag ctcagcaata gatattccaa gagccatgta
     2821 tctggtagag ctggcttttg atttcgcgga aaaaatgtta aaacccggtg gtaatatgct
     2881 agttaaaatt tttcatggct cgggttttga tgaattagtc aaacaggcac gagcatcttt
     2941 tgaaaaagtg gtaatccgta agccatccgc ttcaagatct cgttcaaaag aaacctattt
     3001 gctggcaaag ggctataatt tatagtagtt ttatatatat ttataaatat gcttagcgtt
     3061 gttttggatg atatgagctg tgcaagagct attttgggtc tcgcaacaaa attgtaagta
     3121 actaacgagg ttaatacatt gaacgacatg gttaaaaatt tatttttatg gctgataata
     3181 gcgatagttc tcgtctctgt tttcagtaat tttggtcctc gtaactcagt tgctgagaaa
     3241 atttcttata gccagttttt acaggaagtg gatcaaggga tggttaactc tgttacgatt
     3301 gaggataaca aaatcatcaa gggcgtaacc aagaataata aacgctttgt cacgtacatg
     3361 ccgatgcaag acaatgcttt gttaggcgaa ttgttgaaaa gcaaagtcga tgtcagtggt
     3421 caggaaaaac aacaggaaag tttccttttg catttattta ttaactggtt cccaatgttg
     3481 ttattgatag gtgtatggat atttttcatg cgccaaatgc aaggcggcgg tggccgcggc
     3541 gcaatgtctt tcggcagatc acgagctcgg ctattaggtg aagatcaggt taaagtcacc
     3601 tttgcagatg ttgcaggtgt tgatgaagcc aaagaagaag tgaaagagct tgtcgatttt
     3661 ttacgtgatc caacaaagtt tcaaaatttg ggcggacgca ttccccgcgg tgttttgctg
     3721 gtcggttccc ctggtacagg taaaacatta ttagccaggg cagttgccgg ggaagcgaaa
     3781 gtccctttct ttaccatttc aggttctgat tttgttgaga tgtttgttgg ggtcggtgcg
     3841 tcccgagtgc gtgatatgtt tgagcaagcc aaaaagcatg cgccatgtat catctttata
     3901 gatgaaatcg atgcagtagg tagacacaga ggcgccggct tgggtggcgg tcatgatgaa
     3961 cgcgagcaaa cacttaacca attgcttgtt gaaatggatg gatttgaagg taatgaagga
     4021 gttattgtcg tagcggcaac taaccgtccc gatgttttgg accccgcgct tttacgtccc
     4081 ggccgctttg atcgtcaagt cgttgttcct ttgcctgata tcagaggtcg tgagcaaata
     4141 ttaaaagttc atttacaaaa ggttcctgta gatagccatg tagaagtgaa agctattgcg
     4201 cgcggcaccc caggtttttc aggagcggat ttagccaatc ttgttaatga agcggcatta
     4261 tttgctgcca gggctaataa acgcaaagtg ggtatgattg aactggataa agcaaaagat
     4321 aaaattatga tgggggctga aagacgctcc atggtaatgg atgataacga gaaaaaactc
     4381 accgcatatc atgaagcagg ccatgctatc gttggtctgt cagttcctga gcatgatcca
     4441 gtttataaag tatcaataat tcctcggggc cgagctttgg gtgtaactat gtttttacca
     4501 gaacaagaca gatatagtca cagcaaacgc cgtttggaaa gtcaattatg cagtctgttt
     4561 ggtggtcgaa ttgcagagga attaattttt ggtcctgaaa gtgtgacaac gggcgcttcc
     4621 aatgacatta tgcggtctac tgagattgct cgcaaaatgg ttacaacttg gggcttatct
     4681 gctttaggtc ctctaacttt tggtgaggaa gaggaagaaa tatttttagg acgctcagtg
     4741 aataaacata aggagatgtc cgatagaaca gcacagcaaa ttgatgacga ggttcgtgct
     4801 attattgaca ggaactatca aagagcaaaa gaaatattgg aaactaatat cgataaatta
     4861 catttaatgg cacaaagcct catcaaatac gaaacgatag acactaatca aatccaagaa
     4921 attatgtctg gtaaagagcc tacccctccg gaggattggg ggtcaacaaa accgatggat
     4981 aaggctgagt cagttaatga tgctcctgca aagcctatta atggcgaaac aatagaaaat
     5041 ccggcagaag gacattaatt ttatcagtag ttatttatcg agagtaccct gtgaattctg
     5101 aacagttcct cggttggctt gagcgaaaga gccaacctcc ttcctcctta gtttttgaaa
     5161 agccattaat tatgggtgtg ctaaatgtta cctcagactc attttatgat ggtggcaagt
     5221 atctgtctgt tgatagagcc tgtgatcagg ccttaaaatt aatcgcatgt ggagctgatc
     5281 tcattgatat tggtggggaa tcaaccaaac ctggcgctcc gccaatccct ttagatgttg
     5341 aactatcaag agttcttccc gtaatcaagc aacttcgagc gctcgcagat gtgtgtattt
     5401 ctattgatac taataagcca gaggtgatgg aagcggcaat caatgctggt gcgaatgtta
     5461 taaatgatgt ttatgcttta agaagggatg gtgctctgaa tgttgctgcg cagttagatg
     5521 ttccggtttg tttaatgcac atgaaaggag agccacgaac catgcaagaa aatccttctt
     5581 acccagaagg aataatgaag gaattacagc atttttttga agatcgcatc gataaatgcc
     5641 agaaagccgg gctgaataag aataaattaa ttcttgaccc tggctttggt tttggaaagc
     5701 gcgttaaaga taacctaaaa ttaatttatc atctgaacga attgcgttct tttggattgc
     5761 caattctttt gggcgtttca cgcaaaagca caattggtgc tgtattgaat aatgaagttg
     5821 agcaacgttt gattggaagt attacgctag ggatatatgc tgctcttaaa ggcactgcaa
     5881 ttatcaggac acatgatgtg gatgaaacga atcaagcatt aactatgatt aatgcaattt
     5941 atcgagctgc taaagaaatc agttaatagc tcatgagatg atcagaagac aactgagact
     6001 agaaaaatta aataataagg taggtaagga tgagtcaacg taaatatttt ggtacggatg
     6061 gaatacgggg tcacgttgga ttatcaaata ttaaccctga atttgtttta aaattaggct
     6121 gggcagtagg ctgcgttctt gcgaacggag cgaggaaaaa ggtagtgatt ggtaaggata
     6181 ccagagtatc tggctatatg ttggagtcgg ctttggaagc aggtttgtcc gcagctggag
     6241 ttgatgtggc attattagga ccaatgccta ctcctggaat agcctatcta acacaaacat
     6301 tgagggctaa tgcaggcatc gtgattagcg cttctcataa tttatttgaa gacaatggca
     6361 ttaaattttt ttctgctgat ggaggaaaac ttccggatag tgtagagctt gcaattgaag
     6421 cccaattaga aaaacagttg caaactgtac cttcagccaa gcttggtaaa gcaacacgta
     6481 ttaatgatgc agcgggtcgt tacatagagt tttgtaaatc tacgattcct tccctatctc
     6541 gactatccaa tttgaaaata gttgtggatt gcgccaatgg ggcaacctat catatcgccc
     6601 caaatgtatt ctctgaattg ggtgctgatg tagttcctat aggaattaaa ccagatggtt
     6661 ttaatatcaa tcaagaatgt ggttcgacag cgcctgaatt attaagagaa aaggtgattg
     6721 ctgttggggc tgatattggt attggtttgg atggagacgg ggatcgagtt attttagtgg
     6781 attcgctggg aaatctcgta gatggcgatc aaattattta tatcattgcc aaggacagac
     6841 accaacgagg agtactgcat ggaggggtcg taggcacgtt gatgagtaat tatggtcttg
     6901 agttagcaat tacctcattg ggggttcctt ttcaacgctc aaaagtaggt gacaggtatg
     6961 ttcttgaaac gctaagagag aaagattgga agattggagg tgaaacttct ggacatattg
     7021 tttgtttgga taaaacaaca acaggtgatg gaattgtagc agcactgcaa gttctttcaa
     7081 tcatggtgaa gcaaaataaa gcactgcatg agttaactgc aggtattcaa ttgttacctc
     7141 aaactttagt gaatttaaaa acgaataatg ctgccttgtt agcctctaat cctgatgtaa
     7201 ttcaggcggt taaaaatttg gaaaaacatc ttaatggaga gggtcgagtg ttactcagac
     7261 cttctggcac tgagcctcta ctgagagtta tggtggaagg tgctaatgca tcgatagtca
     7321 agcaacaagc acaaatgtta tgtgatgaca tcagtcagat cgataaaaaa atgactgaga
     7381 gcctaccatc cacttgagca tctgtctttt taagttctaa tgcaggggct tttccaaaag
     7441 cccctctgtt tcacagtatt aaggtattaa ctacttgttg gagccatgga ttggagcatt
     7501 gtgtcagcct cttcaagtaa tcggtgagat cttgttttga aaaaacaagg cctttttgaa
     7561 tactcatatt catcttgccc tgttaactta cctattaaca ttcggaaaaa atcttcaata
     7621 tattcccatg cttttataaa tcctctatgc cgttttaaag gctcatatcg gggatcttga
     7681 gtaatgaggg gcatggcttc tcggcttgaa gtttttgcat cagggagttc catcaaatgt
     7741 ttaattgaaa taagtgaatg agttgcctga ataaataatt cagcttccaa tggatgttcc
     7801 tttttcaatt ttacatattg ctgatgaaat ttttgcaatt cttcaggcca attctttaac
     7861 tgctgttctt catcaatcga ttcatcaagt ataggcaaag caggttttaa tcggagaata
     7921 ttttgtatat cttgcaatgg gtttgggcac atgcgcttgt atgccagtaa tacagataaa
     7981 tcattttgat tttttaattc aaacaagatg tctttgaaaa gatgaagtgc tttaagaccc
     8041 aatgcctcat ttaaacaaga actttgcgct gcatcatata aacctttata ccagagctgt
     8101 atttcttctg aatgttgctt tatatattcc atgttttgta catgaaatga atctaatttt
     8161 tgcttaagca ggatagaaga tgatggcaaa ggggagatgc taggagcaga ttcctggtgt
     8221 tgaggatttg ataatttatc taactcatgg cctgtagaaa caggcttttc tttaaatgct
     8281 ttatcaattc ctaaagaatt tacttttcct ctgtctatgg caagcgtctc agaatcttct
     8341 tcagatgaga tactaatttg attaccagcc tcatcaaaaa aggttaaaat attatcttct
     8401 gataattcat cgtgaccatt gaataatgac aagccatttg gtttcctgga tataactgga
     8461 acctcagggg ttgatggtct caatggttcc gatgggcttg atttgacaac agccagtgtt
     8521 tcttgtccgt tgggtacttg ttgttcttca ctggactcag agccttgctg aaccacttgg
     8581 gtgcggagtg ttaattccgg aacagcaggt atttcttgtc ctatctcttt atgctgcttt
     8641 tcaccagaaa gcccagagtc tttctgaacc ataggagtgg ttcgtgttga ttcaagaacg
     8701 attggtgtat cttgtctctc agttacttgc tgtttttcta cagtaactcc ggaatccttt
     8761 tcgaccatag atgagggccg tgtcaattcc agtacgactg gtatctcttg ctctacttct
     8821 acacgctgtt ttttaccagc agattcagac tcagtatgaa tgatttgagg agggtgtgtt
     8881 gactcgagaa caaccggtat ttcgtgtcca ttgtctacgt gcagtttttc agtggtagat
     8941 tcaagttcct tatgaatgat ttgaggaggg catgttgaat ccagaacaac cggcatttct
     9001 tgtccattgc ctacttgcag tttttggctg atagattcag actccttatg aatgacttga
     9061 cgggggcgta atgattccag aacaatcggc gtttcttcct cattgtctag ctgccgtttt
     9121 tctgggtgag aatcattgcc tatctgaatt acttgggcag ggtgcatcaa ttccacagta
     9181 agagggattg ctttatctac cgctatttcc tgtttttcca atggaggatc gattttttca
     9241 ccggtcagaa taggcaaagg taattgattt aattcagaca caagggattg gatttgattg
     9301 gcaagagtag ataaatcttc tttaatagag tttatgtctt cgttctttag tggttgattt
     9361 gaaattaaac gcaattgctc aataagtgct tttgcttcat tggttgtttg tccatgatct
     9421 ttcataagca aatcaatttg cttggctgtt tcaatataag attggcatag tttaatctgg
     9481 ctttttaact cttctttaag aggatcctct tcattcaatg aatctttatt ttcttcagct
     9541 tcttgcaaac ttggaagttg cacatccata gttataggta aatcgtatcc acttcttagt
     9601 tgttgtattt gtttgcccag agcaggcatt tcttgaagtt tttccaacgc ttgtgaaaat
     9661 tgttccttct tttccgaaac ttttggcatg agaatgagaa gtaccaaata aaaatcggcc
     9721 ggtgaagagt cataagaaag gccacttggt aaaacaaaat ttggtatact ttctattggt
     9781 atttcattta tcaggtcaat ggcatcagga actaattttt tataaataga tatatcctct
     9841 tcatttgaag gagtttctgg tatcgcttta atcaacagtt cttgctgttt cacaagttca
     9901 tgatgcttga tttttagctc tttttgttga gctaataatt gcaggaagct aactgaattc
     9961 ttaagttttt gctgattatc agaataatta gaatccgaat accatgaaag ccaggcagat
    10021 gggttaaaat tagataataa tccatcaatc tggttttggt attcctgtat caatccttga
    10081 gggtcttcac tttcttgaaa tttcctggtt aactctgctt ttaaatcggg tgccagggaa
    10141 tggttagtta ctacggcttc aatacgaccc aggtctttaa gtaatagttc aatttgtttt
    10201 tctagctcag tctctttttt attaatatca gttgcagata attgtttggc gaatcgcttt
    10261 tgcaattcat cccatttttc acaagcgcga gttaattttt taatttccaa ttcaactgca
    10321 gctataaatt tttgtaaact atcaacagca tcttcttcca cagggggttg aatgcccaac
    10381 tgagacaaat ctgtcattaa ggcattccat ttatttttga gcaaaaccaa ctttaatttg
    10441 gcacgtgtag tttctatttc cttactggtt ttgaagagat aactgtagtg cccgactaca
    10501 attgattttt gaacctctcc gtattcaggg aatttataag gaggcgctaa tggattttgt
    10561 ttggacagga cttcgttcgc tttaggttcg atttctttgt ataaaggatt taattcattt
    10621 tttgcgtgtt caattttttt agccaaatca gaaatttttc tttgcagttc ttctcctttt
    10681 ttcatgacct cgtctagttt gtgttttttc cttttatact cttcttgttc ttcagctaaa
    10741 ctggaatatt catcaacata ttttttaatt aacaataaaa gatttaattg ggattcaact
    10801 tcaacaggag cttttccttc cggcagtagg gttgaactaa atatttgctt taaatttaag
    10861 gggttaattg gtggagtcaa ggtagagatt tcattcatta agtgcagtat gatttcgttc
    10921 attataaaac cctcctggtt aaatcatagt aggattctat cgaaatcaaa ccactaagca
    10981 agtagaaaaa tgtatttttt gcaaaaaaaa tgaataatta attctgaaaa aaaacataga
    11041 taaagctaca catctgagcg aagtttcggt atattgcaca gctgcacatt atcagttgtt
    11101 tactgatagg ctggaaataa tagaaaggat ttggtccgcc tatactagaa cttgttggag
    11161 ggagtgtgag gcaaaagata gttgctggta actggaaaat gaatggccag attcaacagg
    11221 tgactgaatt ggtttcgcaa attgaggaat taattggctt tgattgcgca gcacaggttg
    11281 ctgtaatgcc tcccagcatt tatataccca aggtaagaga ctgcttaagg acggggaaga
    11341 ttgtagtagg cgcgcaaaat gtttatccaa aagattatgg tgcttacaca ggtgaactgt
    11401 cagctcccat gcttaaagat tttgattgcc gatatgttct ggtggggcac tcggagcgca
    11461 ggcaattttt tcatgaagat gaaaattttg tggcgcaaaa attccaccat gtaaaagatc
    11521 atggtatgat acctattctt tgtgttggtg aaactctttc tgaaagagag aatggaaaaa
    11581 cagagcaggt tattgctcaa caggtgctcg cagtgagtgc aaaagggaaa gattgttttc
    11641 gtgattgcgt agtggcttat gagcctgtat gggcaattgg gacaggaaaa actgctacac
    11701 ctgaacaggc acaaaaaata caccagttta ttagagatct ggttggagaa ataaatgata
    11761 gcgatgcgaa acatttgacg ctcatatatg gtggcagtgt taatgaaaat aatgcaaaag
    11821 ccttattttc catgccagat attgatggag ggttagtggg tggagcatcg ttgaatgcaa
    11881 aacaatttgt ggaaattgtg aaatgtatca attgatatta atgattcatg tcttaattgc
    11941 tgtaatttta ataggcttgg ttcttatcca acatggtaag ggtgctgata taggtgctgc
    12001 ttttggttct ggcgcatcaa acacgttatt tggaagtcaa ggtactggtg gcttcttgtt
    12061 caagttaact ggtggtctgg caatgacttt ttttattacc agtttgatgc tgtcttatat
    12121 ggtttctacg cagaaccaaa aggcaggaca gttagctatt cctcaacaaa ccggtgctcc
    12181 agtcagttca gtcccagtgc cagttgaaaa aaataataag aagtaactaa aaagatgtag
    12241 tgtctttagg gctgttgtct tttgtgatga actcaggata acaaaaataa aaataagccg
    12301 aagtggtgaa attggtagac acgccgtctt gagggggcgg tggcgcaagc cgtgccggtt
    12361 cgagtccggc cttcggcacc attttaaatc gagcaaggtg aactgatgct atctcaatat
    12421 ctacccgttc tcgtttttat aattgttgcc ttgattttag gtgcagtaat gataggcgct
    12481 ggttctttgc tttccaagca tagtccgaat agcgctaaaa actcccccta tgagtgtggt
    12541 tttggcgcct ttgaaagctc ccatattccg tttgatgtac ggttttatct ggtggctatt
    12601 ttatttatta tttttgatct ggaaacagca ttctttttcc cttgggcctt ggcattacgt
    12661 aaaataggat ggtttggctt ttatgctatg atgttatttc ttggactgtt ggttataggt
    12721 tttatttatg aatggaaaag aggcgccctg gagtgggaat aaggttcttt cattctttta
    12781 aaatatttga tttaatcttt cgttttagcc attactatgg gaagcaaagg acgcttagca
    12841 aatcattaca gagctttttg cttttagagg aacctgatgg attttaggtt ggtttttaaa
    12901 tcctggttgt cctttgccaa aacgatagct gagattatat tttaagccat tgaataatgg
    12961 ttatataaaa actgggttat taattattta actgcttaga ggcgaactat ggcggttgca
    13021 gagctagaaa aaaaaggttt tgagttgact acggtcgaaa aattggttgg ttgggcacgc
    13081 agtggctcta tgtggccaat gacatttggc ttagcttgct gtgccgtgga aatgatgcac
    13141 gtgggagcag cacgctatga tttggacagg tttggtatta ttttccgtcc cagtccacgc
    13201 cagtcagatg tcatgattgt agcagggact ttatgtaaca aaatggcccc ggctctcaga
    13261 aaagtttatg atcagatgcc agaaccacgc tgggttattt cgatggggtc ttgtgccaat
    13321 ggtgggggat attatcatta ttcctattca gtggttcgtg gctgcgatcg tattgtgcca
    13381 gtcgatgtct atgttcctgg ctgtccacca acagcagaag cgttgttata tggtattatt
    13441 caactacaga ataaaatcag gcggaaacct gttttagagg cataagctca tcaagggtgc
    13501 agattagatg acaaaaaatg agtatttgat tgaaaagcta caagctaatt tagcaaatca
    13561 tatcaccgaa ttgacctccg catatggtga agtaaccatt gagtgtgagg tgcataattt
    13621 attgcctgtt atgattgagt tgagagacag agaggaattt tctttcgatc agttaattga
    13681 tctatgtggc gtagattatt tgcattatgg tgattatgat tgggaaacag agtctgcgac
    13741 agagcatgga ttttcacgag gtgtcgagcg tcaggaagct aaggcttatg ctgtaaataa
    13801 accacgattc gctgtagtct atcatttatt atcaaccaaa aaaaatcatc gtttacgggt
    13861 caaattattt gtagaagaat cccatttaat tgttccatca gtacatcatt tatggaagtc
    13921 tgctaactgg tttgagcggg aagcttatga tttatatggg attttattcg atggccatcc
    13981 tgatttaaga cggttactga cagattatgg ttttataggc caccctttcc gcaaggattt
    14041 tccactcagt ggagaggtgg aaatgagata tgatgcaaaa ctgcaaaagg taatatatgc
    14101 ccctgtagac attgtgccaa gaattgtagt tccaaaggtt attcgtaacg ataaccgcta
    14161 cataggtaat gaaggtagca aaaatgattg aacttaaaaa ttataccttg aattttggcc
    14221 cacaacatcc tgctgcacat ggtgttttaa gattggttct tgagttagag ggtgaaacta
    14281 tcgttcgtgc tgatccacat attggattgc ttcatcgtgc aacggaaaaa ttggctgaga
    14341 ccaagcctta cattcaaagc attggttaca tggacagatt agattacgtc tcaatgatgt
    14401 gtaacgaaca tgcttatgtc atggcgattg agaagttatt gggtatagaa ccgcctttgc
    14461 gagccaagta tatacgaact atgtttgatg aggtgactcg aattctgaac catctcttgt
    14521 ggttaggagc tactgcattg gatattggtg caatgacagt gtttctttat tgtttcagag
    14581 agagagaaga tttgtttgat tgctacgagg cagtttcagg agcacgtatg catgctacct
    14641 actataggcc aggaggggta gcaagagact tgccagatac tatgcctcaa tacaaaccgt
    14701 caagatggca taacgaaaga gagattgaaa aaaagaatca caatcgtcag ggcagccttt
    14761 tggattttct ttgggccttt actgagcgat ttccccgctg tgtggatgaa tacgaaacat
    14821 tgttaaccga taatcgtata tggaaacaac gtactgtaga tattggtatc gtttcacctg
    14881 aaaatgcgct gcaatggggg tttaccggac caatgttaag aggctccggt attgcctggg
    14941 atttacgcaa gaaacaaagc tatgctgctt acgacagggt tgattttgat attcccgttg
    15001 gcaagacagg ggattgttat gatcgatatt tggtaagagt cgaggaatta agacaatcca
    15061 atcgaattat aagacaatgt attgagtggt tgcgaaagca ccctggccca gtaaaagtcg
    15121 atgattataa agtgacgcct ccacgtcggg tagtaatgaa acatgacatg gaagcattaa
    15181 ttcatcactt taaattgttt accgaaggtt tttgtttacc gagaggtgag gtttattcat
    15241 ctgttgaagc gccaaagggc gagtttggga tctatatggt gtcagatgga gctaataaac
    15301 cttaccgtct gaaaataaga gccccaggct ttgctcattt atcaagcttt gatgatatgg
    15361 ttagaggcca catgctagct gatggagtgg cgatattggc aagtcaagat atagtgtttg
    15421 gagagataga tcgttaattt aacgacatgc ttgcttaaag tgtattaaga gataataaac
    15481 gatataaagg tttcgaaatg tctgctaatt cagcaaaaat attaaatcaa ttgatgccgg
    15541 cgaaccgtat gcgcgatatt gatcattgga tatcaaaata tcccaaagat caaaaacaat
    15601 ccgcggtcat gagtgctctt cgaatagtac aagaagaaca tggtcattta acaacggaat
    15661 taatgaatgc agtagctgaa tatcttgata tgccgcccat cgcagtttat gaagtggcaa
    15721 gtttttatac aatgtatgag cacaagccag tggggcgaca tttaattaat gtttgtacca
    15781 atatttcttg catgctgagg gattctgctg gcgttgttga acatttagaa aaaaaattgg
    15841 gtgttaatct aggtggaaca acagaagatg gtcgatttac tttaagatca gttgaatgct
    15901 taggtgcttg tgtgaatgcc ccaatgatgc aagttgataa agactatcac gaaaatttga
    15961 ctgctgaatc tatagataaa gtattggaac agtaccaata gaaaataaag gtgtttgcca
    16021 tggttgaact aaatcaagtt tgttaccgaa catttcattt accagagccc tggtcgctat
    16081 caacttatga aagtattggt ggctatagcg catggcgaaa aattttaaaa gagcaaacat
    16141 cacctcaatt aataattgaa gagttgaaaa cttctgcctt gcgcggacga ggaggggctg
    16201 gattcccaac cgggttaaaa tggagtttta tgaatagaaa ctcccctgtt caaaaatacg
    16261 tagtatgtaa ttctgatgaa ggcgaaccgg gtacttgcaa ggacagagag attcttactt
    16321 taaatcctca ccaattaatc gaaggaatgg ccatagctgg gtatgttatg ggcgccacgg
    16381 ttggttacaa ctacataaga ggagaattct ggctgccatt tgagcgtact gaggctgcaa
    16441 taaaagaagc atacgatgct ggcttgctag gtaaaaatat attaggctcc ggcgttgatt
    16501 ttgatttata taatcattta ggagcgggag cttacatttg cggtgaagaa actgcattgc
    16561 ttaactcaat tgaaggaaaa aagggaatgc ctcgttttaa accaccattc ccggcaaatt
    16621 atggcttgta tggtaaaccg acaaccataa ataacactga aacatttgcg tctgttccgg
    16681 taattatgga aaaaggtgga gaatggttct taaaattggg aaaaccgaac aatggcggtg
    16741 cgaagtgctt tagtgttagc ggccatgtta acaaacctgg taactatgaa attcccttag
    16801 gcacgccatt caaaacgctc cttgatctgg ctggtggcgt cattaaaggc cgaaaaatta
    16861 aagctgtgat tcctggtggt tcctctatgc cagtgctccc aggtgacgtc atgctggggc
    16921 tggatatgga ttatgacagt atacagaaag ctggctcagg cttgggttca ggcgcggtca
    16981 ttatcatgga tgaaaccacc tgcatggtgg atgctttgta tcgtatttct gagttctaca
    17041 tggatgaatc ctgtggacaa tgtacacctt gtcgagaagg cacaggctgg ttagtcaggt
    17101 tattacacag aattataaac ggtcatggcg agccgggaga cgtggagaag ttagtgaatg
    17161 tggcgaataa catagaggga agaaccattt gtgcattagg cgaggcggca gcttggccag
    17221 tacagagttt tgttaaacat ttctatgatg aatttgaata tttcataaag cataaacgct
    17281 cgatcgtcgc agcataatta agaaggttag accatggcta ctattgaaat cgacggtaaa
    17341 acatttgaag cagaaaacgg taaaatgatt atagaggttg ctgatgaggc gggcatctat
    17401 attccacgtt tttgctatca taaaaaatta tctgtggcag ctaattgccg tatgtgtttg
    17461 gtcgaagtgg aaaatgggag aaaaccggtt cctgcttgtg ctacacctat aaccaatggg
    17521 atgaaagtat ttacaaagtc agagcaggct atacactccc aaaaggtagt gatggaattc
    17581 ctcttgatta atcatccttt ggattgccct atctgtgatc agggtggaga atgtgagctt
    17641 caagatattt ctatggggtt tggcgctgat aaatcagagt atacagaaag caagcgtgcg
    17701 gtagacgatg agaatttagg aacgctaata tctaccgaaa tgacacgttg catccattgt
    17761 actcgctgtg tacgcttcgg cgaggaaatt gcgggtgtaa gagagctggg agctaccggc
    17821 cgtggcgaaa agacgcagat tggtacttat gtagaacaca gtatgacatc ggaagtttca
    17881 gggaatatca tagacttgtg ccctgttggt gctttaactt ccaagcctta tcgttttaaa
    17941 gcccgtgcat gggaattgac ccaacacgac agtgttgcgc ctcatgattg cttggggtca
    18001 aatgttcata ttcatacaag aaataataaa ttaatgcgtg tagttcccag ggaaaacgaa
    18061 tcgatcaacg aaacctggct ttcagacaga gacagattta gctatttggg attaaatgca
    18121 acttcaaggg caagcaaacc acaaatcaaa aaaaatggcc aatgggaagc agttgattgg
    18181 gaaactgcgc ttaagtttac cgcagaaggg ataagccgag ttatcaaaca acatggccca
    18241 gagcaaatgg ccgcttttgc ttccagttct tctaccttgg aagaaatgta tttactacaa
    18301 aaattaatga gagaactagg tgttcaaaat ctagatcacc gattacaaca aattgatttt
    18361 agagatcagg catttttacc tacaacacca gtaagcactt taccctacgc tgagatagat
    18421 cagcagcatt caattctgtt aataggatgc aatattcata gagaagtacc attggcagga
    18481 acacgggtaa gaaaggcttt tcgcaatggc gcaaaaattt atgcccttaa tcctgtagat
    18541 tttgattatc attttgattt gtccggacgg gtcatcattt ctcctctgga aatgccgatg
    18601 caattagcta aattagcctt agctcttaca tccgagttag catcgcttcc agaagaagtt
    18661 caaaaattgt taataggttt ggaagtagat aaacaaacca aacaaatagc acaatcatta
    18721 aaagaagaga aagcctgttt gataacagga gcaatagttg aaaatcatcc agaagcttcc
    18781 ttattgcgta ctttggtcgc tattgttcaa aagctgagcg gagccaaatt agtcagatta
    18841 acgactggag caaacagcgc gggcgcatgt attgcaggta tgctgccgca tagaacagtt
    18901 gctgggaaat caattgcaga gccaggtttt aatgtacaag aagcgttaaa ttccaagtta
    18961 aaaggttatt tactcatggg cgttgaacca ggctatgatt ttgcaaatcc agccggggca
    19021 aggcaatcaa tgttagctgc agagtttgtt gtactcttat cagcctatga gcatgaatcc
    19081 atgcatgatt atgcagatgt tattttaccg atcgctcctt atgctgaaac atctggcaca
    19141 tacataaaca tcgataatac ctggcaaaca gtgaaagggg ccatgctgcc acttggagaa
    19201 tcccggccag cctggaaggt gcttcgtgtt ttgggtaatt tgttgcactg taaaaaattt
    19261 gattatacct caacagagga catcttggag gaagtcaaag aagcagtatc gatgacaatg
    19321 gagcatgaat acgaacctta ttatcctgaa tcattgcctg tcattaatca atcactggta
    19381 agagttggag aatggcctct ttatcgaata gatgctatta ctagaaatgc aaaagagtta
    19441 caattatgtg ccgcatccga atctgcctgt ataagaattc atccatcaac tgcagataga
    19501 ttgaaattag aagaaattgc tactgtatct caaggagata ttgagataac attgccgcta
    19561 aaacgtgatg agcgcattgc ggttgatgtt gtgtgggtgg caaatgcaat gccagaaaca
    19621 gttgacctcg gtcactcgtt tgctgcaata acaataaagc gctagaaagg taaaaaaatg
    19681 cttcatacta ttggcatact aatttggatt attattaaaa ttcttgtgat agttgtccct
    19741 ttgctgcttt ccgtggccta cctaacctat gcggagcgta aagtgattgg ctatattcaa
    19801 gtgcgtatcg gcccgaacag ggttggttta aagggcttgc tgcagccatt tgctgattta
    19861 ctgaaattaa ttactaaaga aatcatagtc cccactcgat caaataaata cctttttgtt
    19921 attgctccac tttttgcttt agtcccttcc ttagtcggtt gggcggtaat cccttttcag
    19981 gaaggtgttg ttctggcaaa tattaacgca ggggtcttat acttatttgc catgagttct
    20041 ttgggtgtat atggtgttct tattgcaggg tgggcatcga attccaaata tgccatgttt
    20101 ggggcgctac gtagtactgc ccaaacagtc tcttacgaaa ttgcaatggg atttgctctt
    20161 gtcggggtac tcctggctgc aggaagcatg aacttgactg atatcgttaa ctctcaaaaa
    20221 ggcggaatgc tgcattggtg gtttattccc ttacttcccc tattcttggt tttctggatc
    20281 gccggtattg cagaaactaa cagagctcct tttgacttgg cagaaggtga atcagaaatt
    20341 gttgctggtt tccatgtaga atattctggt atagggttcg ccttattctt tctctctgaa
    20401 tacgccagta tgattttgat ttcaacattt atggctattt tattcatggg cggatggctg
    20461 tcgccttttg aaggcattac gttcctggat caaatctttt ttgtagtacc cggatttgtt
    20521 tggttacttc tgaaaattag tttcttcctt tttgtgtatt tgtgggtaag ggctactttc
    20581 ccccgatatc gttatgacca gcttatgcgt ttaggctgga aggtacttat ccctgtgact
    20641 atagtttggt taatagtaac ctccctcatg gtggttgctc atgtcaagcc atggttttga
    20701 cggttagaag agaagccgat gaaaaaagta tatcattata taattcacta tgttcgtacc
    20761 tacttactgt tagagctatt agccggttta tggctaacag taaagtattt tttcaggaaa
    20821 aaaataacag ttcagtttcc tgaagaacaa acgccacttt ctccaagatt cagggggctt
    20881 ttggcattac gccgttatcc caatggtgaa gagcgctgca ttgcatgtaa attatgtgaa
    20941 gcagtttgtc ctgcattggc tattacaatt gaatcagagc aaagagaaga tggatcacgc
    21001 cgtactactc ggtatgatat tgatatgttt aaatgtatta attgtggatt atgtgaagaa
    21061 tcttgccctg ttgattcgat cgttgtcact ccaattcatc actaccatat cagcgaacgt
    21121 ggtcaaaata ttatgacaaa agagaagctt ttagcagtag gcgatttgat ggaaacacaa
    21181 ctcgcagcag acagagctgc tgatgaaaaa taccgttgat ggggtgattt atgcatgaat
    21241 gggtaatcca agcaattttt tacatctttg cattattgac cgtattgtcg gcactaatgg
    21301 taataagcca aaacaatcca gttcgttgtg ttttgttttt agtgctcact ttttttacca
    21361 gtgcggtttt atgggtttta gctgaggctg aatttttagc attaatcctt atcctggtat
    21421 atgttggcgc tgtcatgact ttatttctgt ttgtcgtcat gatgttaaac atagacatcg
    21481 aaagcatgaa aagtcattta attaaatatc taccatttgg attaattata gttgccttgt
    21541 tgaccgggtt attaatggta gctatcccta aagatttatt taagaacagt gtgcaaattc
    21601 aagaaaagcc tttgagtgtt agctcacaat taatggattt atctactcaa gatgagctgg
    21661 ttccacctga aaacgctaca tcgaatactg aaaagttagg aatggttctt tatacagatt
    21721 atattcttgc ctttgaaata gctgcagtca ttttgctggt agctattgtg tctgcaatta
    21781 ccttggtgca taggggcgct atcaggtcaa aacgacaaga tatcactcag caaattatga
    21841 ctcgaagggc agaacgagtg aagttgataa gtatgaagtc agagaaatag ttattgggga
    21901 attctttatg atacctgttt atgattattt agtattagga gtgattttat tcggcttgag
    21961 cctggttggt attatgctca atcgtaaaaa tatcatctta ttacttgttt gtgttgagtt
    22021 gatgctcttg gctgtgaata ccaattttat tgcattttct cattactaca atgaagtagg
    22081 cggacaggta tttgtctttt tcattttaac tgtagctgcc gctgaagcag caataggttt
    22141 ggccatagtc atgttgcttt atagaaacag aggcaatatt gacgttgata aaatgaatca
    22201 tttaaaaggt taaaatcgtg agtattcaac aagtctgctt agtaatcgtt ttgactcccc
    22261 ttgttggttc tgctatagca ggatttttta gaaaccagat tggacgagtc ggagctcata
    22321 ctgtaactat tttaggcgta gctatttcct tagtatgttc aatttttctt gcgtggggcc
    22381 tattttctgg ttcgatccca aacacaaata gccttgttta tcactgggct agcggggggg
    22441 ctttaattcc ctacgaattt aatattggct ttttaataga cccgctaagc gttgtcatgc
    22501 tggttatagt taattttgtt tctttactag tccatgtcta cagtattggg tatatggcag
    22561 atgatgatgg ttatcaacga ttttttagtt atatctcttt atttacattc atgatgctca
    22621 tgttagttag cgcaaacaac tttctgcaat tattttttgg ctgggaaggg gttgggctcg
    22681 tttcttattt attaatcgga ttttggtatc aaaaggaatc tgcaatagaa ggcagcctga
    22741 aagcatttct tgtaaaccgt gtaggagatt ttggatttgt actaggaata ggtttaattt
    22801 ttgcctatgc tggaagtctt gattatgaaa aagtatttac cagcgcaaat tatttggcaa
    22861 gccagaatat agagttattt agcggctact cctggtcact gattacagtt atctgcctgc
    22921 tgctttttgt tggcgccatg ggcaaatcag cacaagtacc acttcacgtt tggttacctg
    22981 agtcaatgga gggtccaacc cctatttcag cactaatcca tgcggcgaca atggtgacag
    23041 caggtgtttt catgattgct cgtatctcac ctttaattga attgtctaca gctgcgctta
    23101 gcacagtatt agtgatcggg gctacaggag cattgtttac aggaatactg gctttggtta
    23161 tgaacgacat caaacgcgtg gtggcctatt ctactttatc acaactaggc tatatgatgg
    23221 tggcaatggg tgcctcagct tatagtaccg gcatgtttca tttattgaca catgcgtgtt
    23281 ttaaagccct attgtttctt ggtgctggtt cagtcattat tggcatgcac catgaacaag
    23341 atatgcggaa aatgggcggg ttatggaata aaatgccgat tacctatgta acctatgtca
    23401 taggcagttt ggcattgtgt gcatttcctc cttttgctgg cttttattca aaagatacca
    23461 ttattgaagc agcgcagctt tcccagatac ccggcagcag ttatgcttat ttttgtgtaa
    23521 cagcaggcgc tatggttaca gcattatata ctttcagatc cttgttcatg acatttcatg
    23581 gcaagcctcg tatggatgaa catacattaa gtcatcttca tgaatctcca tgggtagtat
    23641 ggttaccact agtattgctg gctatcccat caataatttt aggctatgtg ctttacatgc
    23701 ctattctatt tgatacacca accctcttag gctcatcaat ttttgtttta cctgagcata
    23761 atgttttagc agaacttgct catgaagtaa gttcgccatt tgcaagtgct gttcacgcga
    23821 tttattcctt gccattttgg attacagtac tcggagcagt tattgcctgg gtttgttata
    23881 ttgcggttcc atctattccc ggttatttag ccaggtattt ttcaattatt tactctattt
    23941 tacttaataa atacgggttt gatcgcttta acgagctgtt ctttgtgaaa ggagctagag
    24001 gattaggcat tgctttttac aagattggtg atcagaaact aatcgatggt gcagttgtca
    24061 atggtagtgg acgcctggtt cgctggttct ctgggaaagg gcgaaagata caaagtgggt
    24121 acatctatca ttatgcgaca gtaatggtgt ttggattact ggccttttta tgctggttaa
    24181 tcctggactg aagataaggt gaaggtatgc attatttgct caatttatta atttggttac
    24241 caatattagg tggcgttttt gttttgctga ctggggatga taaaaatcca aatgtatcgc
    24301 gttatttaac attattcacc atactcctct gtttagtgct ctgcatccct ttagttaaag
    24361 gatttgatct gcaatctttt ggaatgcagt ttatagagga aatcccctgg atgccggcac
    24421 tgggtattaa ttatactctt ggtattgatg ggttatcttt actattaatt gttttatcta
    24481 tttttactaa tttaatagtg attttggcta cttgggatag cattagtaaa aaagtttccc
    24541 aatatttatc agcgttttta attatgcaag ggcttcttgt tggtatattt tctgccatgg
    24601 atgccatagt attttatatt ttctgggaag caaccttaat tccaatgtat ctgattatag
    24661 gtatttgggg ttctgataac agagtttatg cagcaataaa atttttccta tatacttttt
    24721 taggatcagt tctcatgctg gccagtttcc tgtacatggg ctatattgca ggctcattta
    24781 agatcgaaac gttttatgct atcaaattag gcatgactgc acaaacattg atttttattg
    24841 cctttttctt aggttttgca ataaaaatac cgatgtttcc agtccatacc tggttaccag
    24901 atgcgcatac tgaagcccct gctggtggtt cagtcgtttt agcagcaatt ttgctaaaac
    24961 tgggagctta tggatttatc cgtttttctt taccaatagt ccctgatgct tgcagcaaat
    25021 atgctccttt gatggttgcc ttatctttga tcgcagtagt ttatatagga ctcattgcta
    25081 ttattcagaa ggatatgaaa aaattgattg catattcttc aatttcacat atgggttttg
    25141 taactctagg ttgtttcgct atatttgcaa ttgctcgtca tgggggacta agcaatgccg
    25201 gtttaatcct tgagggtgca attgtcgtta tgatatcgca tgcctttgtc tcaagtgcaa
    25261 tgtttgcagg tgtaggtttt atatatgatc gtatgcatac aagacagctc gcggattttg
    25321 gcggaatagt aaataccatg cctatatttg cttctttttt tatgcttttt gctatggcga
    25381 atgccggttt gccaggaaca tcgggatttg ttggtgagtt tatggtgata ttaggtagca
    25441 taaaggctgg tttttgggtt gcattttggg ctgcaactac attgattatt ggagcagcat
    25501 atacgctatg gatgtacaaa agagttattt ttggtcctgt agctaataaa aaggttgctg
    25561 ccttaaaaga tatatcaggt tttgaattaa gtgcttatgt attgcttgca ataatggtca
    25621 ttgctctagg agtgtatccc aaacccatgt tagagtttgt gcatcaaact gtaagtcata
    25681 ctttggcatt ggctgataaa tctaaactct aacaaagaac tgcgataggc ttaaattgca
    25741 atcaagttcc ttagcaaggt taaatatgat gacattacta gaaaatatcc atgtagcaat
    25801 accagaaatg attattttaa taaccgcgtg tttggcgttg ctttctgatt tgttctttcg
    25861 tcataaatta aaatcaattg cattttatat atcttgtgca ggaattatag tttctgcagc
    25921 agtaagcttt gtatttatag gaagctacaa gttacttata ttcaacaaat tatttataag
    25981 tgatgatatt agtcatttaa tgaaactgtt tattgatatc acggttttat taagttttgt
    26041 ctattcccaa aactatctgg atgaacggca aatgcctaca ggtgattatt atgttctggg
    26101 actgttttca acactaggta tgatgattct tgtttcagca cactcgttgt tgtcccttta
    26161 cctaggtttg gagttaatgt cattgccgct ctatgcaatg accgctatcc gccgaactga
    26221 tagtgatgca tccgaagcgg caatgaaata ttttgttatg ggtgctattg cttctggtat
    26281 gctgttgtat ggaatatccc tggtgtatgg ggcaacagga aaacttgatt tattagatgt
    26341 tgctaatacg gtggctgtca attggcagca acaaaatact ctttttgctt ttgcaatggt
    26401 atttatactt gccggcgtag gattcaaact ggctgcagtc cctttccata tgtgggctcc
    26461 agacgtatat caaggggcac caagctctgt aacacttttt attagtacag cacccaagat
    26521 tgctgcactg gggatggcac tgcgattttt gactattgga ttggtcgaca tcaccatgca
    26581 atggcagcaa attattttag taatggctct cctttctacc ggcataggta atttgttagc
    26641 agtgattcaa accagaatca aacgcttgtt agcttattct gcaatttctc atataggata
    26701 tgccttattt ggaattttag ccgccagctc tgctggttat gccgcagcac tgtattatat
    26761 tttagtctat gctgtaatgt cagcggctgg ttttggttta gttgtattga tgtcaaatca
    26821 tggaatggaa attgatagta ttgatgactt aaaaggcttg aataaaagaa acccgtggct
    26881 ggcgtttatg atgctattag tcatgttctc aatggctggc gtgccgccaa cggttggctt
    26941 ctttacaaaa ttattggtat tgaaggcatt agtagatgtt catatgactt ggttagctgt
    27001 tgtaggtttg ctatttacgg ttataggtgc ctattattat ttgcgaattg ttaaaattat
    27061 gtattttgat caacctgaaa atgaagatat agtaagaatt ggtacaggca ataccatagt
    27121 ttattcactt aattgcctct ccttactata tttaggtatt ttcccaggcg ctttaattgc
    27181 agcctgtatt aatgcatttg caaattaatt aataaacttt aacaaaagta cttgcttagg
    27241 gacccaagta tcagtatact tgccctcagt tgacgcgggg tggagcagcc tggtagctcg
    27301 tcgggctcat aacccgaagg tcgtaggttc aaatcctgcc cccgctacca attttataaa
    27361 accccatttt tggggttttt tattatgttg actaggatac tgtctaaatc taagtcagtt
    27421 accaggcttg ggcgagtgcg cccttttttt gtttatacat actatgataa atgatgactt
    27481 gatagtgcta ttagagccta taatcaaaaa tatgggttat gaactttggg gctgtgaata
    27541 tctttcccaa gggaaacact cgttattaag aatttatata gacaaacctg atggaatagg
    27601 aatcgatgac tgtcaggaag tgagtaagca agtcagtgct atgcttgatg ttgaagaccc
    27661 tattccaggg cattatagtt tggaaatatc atctcctggg ataccccgac ccttgtttag
    27721 tatctggcaa tatcagcgtt accttggtta tgaaattcat gtaaaaactt ttaagccagt
    27781 caatgggaag cgaaaattat caggtattat tgtttcagca tcagaagata caatagtatt
    27841 ggatattaat aatgaacatc aagaaatact tttatcaaat attgtgaaag caaatttgac
    27901 agtctagaga ggcgaacaat gagcaaagaa ttgttattag ttgctgaggc attatcaaac
    27961 gaaagaggcg ttagtaaaga cgtcattata cttgccattc aggctgcact ggaatctgca
    28021 actcgaaaga ttcttggttt agatataggt gtgcgtatca aaatagattc taaaaccggt
    28081 gactatgaaa cctttagata ctgggatgta gtgaatgaag aagaattaga gtttccggac
    28141 cgccaactgt ctttggaaca agcaaaagag cgaaaaccct cggctgctgt tggtgaccga
    28201 attgaagaaa ttattccttc catagaattt ggaagaattg aagcgcaaac agcgcgacaa
    28261 gttattatgc aaaaagtcag agaagcggaa cgtgaattga tcatagacca attccgtagc
    28321 aaacttgggc agctaatcta tggaactgtt aaaaaagtaa ctcgtgacaa tgtaattatt
    28381 gatttaggtg gtaaagcaga ggctttcttg ccacgctctg aaatgttgcc tcatgaaatg
    28441 tttcgtccca atgacagagt tcgagcatat ctgtatgaaa tcacgccgca ggcgagagga
    28501 ccacaattgt tcgtaagcag aactcgaaat gaaatgctca ttgaattatt ccgtattgaa
    28561 gttccggaaa ttggtgaaaa tataatagac atcaaagctg cagcgcgcga tccgggaaat
    28621 agagccaaaa ttgctgttaa aaccaatgat gggcgtattg accccatcgg tgcttgtgtt
    28681 ggtatgcgtg gagcaagggt acaagcggtt tccagcgaat taggtggtga gcgagttgat
    28741 atcattctat gggatgataa ccctgcccaa ttagtcataa atgctatggc tccagctgat
    28801 attacatcta tcgttgtgga tgaagatact catactatgg atttagctgt tgcgaaagaa
    28861 caattatcgc aggcaattgg tcgaaacgga caaaatgttc gtttggctag ccaattaact
    28921 ggttggacat tgaatgtcat gacaacggaa gagtttgaaa gcaaaaatca agaggaatca
    28981 agtaaaatag ttaatctgtt taccaatgcg ctagaaatcg atgaggaaat agcgacatta
    29041 ttggtagctc atggattctc ttctctcgaa gaagtggctt atgttccgaa ggaggagctg
    29101 ctggctattg aagaatttga tgaagagata gttgaagagt taagaaacag agcaaatgac
    29161 aagttattgg agatggcact gtcatcgggc aaaggattat ctggtactcc agatgattcc
    29221 cttatcaata tggaaggcat gacagaggat ttggcaaata aattagcaag taaaggaatt
    29281 acaacaatgg aagatcttgc agagcaatct gtagatgaat taatggaaat agaaggcatt
    29341 agtgaagaaa aagcaggtgc tttaattatg aaagcaagag aaccatggtt tcaataaact
    29401 atggttatcg aaattgacca atacgatgct agatgcattt taaatatatt cagctttata
    29461 aaaagttgct gagaggggat taaatatggc ggatgtgacg gttaaacaat tagctcaagt
    29521 cgttggtatc ccggttgagc gcttattgaa ccagttacag gaagcagggt tatcttttac
    29581 tgatgatcaa cagactgtga atgaagaaca aaagaggatc ttgcttaatc atttaaaagg
    29641 tagctctaat cgtgatatta gtgctgcgcc tgaaagaata actttaagac gtaaaagcat
    29701 gtcgcaagtc actgttgggc atgatatgca tagtggcaaa acagttaata ttgaggttag
    29761 aaagaaaaaa actttcatca agagaagcgc aattcccgaa caagcagaga tagaagaacc
    29821 cgtagttcca cctgtcgttg aagagcccgt ccatgaagaa atcactgtgg ttagtgacgt
    29881 gtctgcagaa actcccgact tgaaagagac agaggagcat ccagttattg aacctgtagc
    29941 agagctcgat gaaacagtaa aagaagaaga aaaaatcagt ttagaggaaa acacagcaga
    30001 gagtcaggat gagttaaccc atgcaaacac tgatgtaata gaaaatcttg tggatgttgt
    30061 cgaggagacc atccctgttt ccaagaagga agaagttaaa ccagagaaag tcagtaaaaa
    30121 gaaacatctg gagcaaactg attctgatat ttctgaattt aaaaaaggta agaaaaagcc
    30181 taaatatcat acttttgaac atgatgagga ggaacaagaa cttcatcgtc gtggtggtcg
    30241 aagtaaattt aaaaagaaaa aaggaactga aaaatcggat aaatacagag aagctgaaga
    30301 aacattaacg catggttttg cattacctac tgctccaata gtcagagaag tattgatacc
    30361 cgagacaatt acagtcgccg aactggcaaa acggatgtca gtaaaagccg ctgaagtgat
    30421 taaagtcatg atgtctttag gagcgatggc aacaattaat caggtcattg atcaagaaac
    30481 gtctgtaatc gttgtagagg aaatgggaca taaaccggta attatcaaag aggatgctgt
    30541 tgaaaccggt ttaggcgaag caatttccaa aggcacaaaa acagaaggaa gagccccggt
    30601 tgttacgatt atgggacatg ttgaccatgg taaaacctct ttactggatt acattcgtcg
    30661 aaccaaggtt gcagcaggtg aagcaggagg cattacgcaa cacataggtg cttaccatgt
    30721 cagtacgcca aaaggtaaca ttactttttt agatacgcct ggacatgccg catttaccgc
    30781 gatgagagcc cgtggtgccc aagcaactga tatagtcatt ctaatcgtgg cagctgatga
    30841 tggcgttaag ccccagacaa ttgaagcgat acagcatgct aaggccgcta aggttccaat
    30901 tattgttgca attaataaaa tggataaacc cgacgctgat cctgagcgag ttatgaatga
    30961 actctccgtt caagaagtta ttcctgaggc atggggtgga gataccatgt ttgttaatat
    31021 ttcggccaaa tcaggtatgg gcatagatga tttgctggat gctattttat tgcaatcaga
    31081 agtattagag ttaaaagcag tgactgatgg tgctgcaaaa ggagttgtca ttgaatcacg
    31141 tctggataaa gggcgaggtc ccgttgcaac ggttttagtt caaagcggaa ccttacataa
    31201 aggtgatatt ttacttgctg gctttcagta tgggcgtgtg cgtgctttgg ttagcgacaa
    31261 tggagatttg gttgatagcg ctggcccatc cattccagtc gaagtacttg ggttatcggc
    31321 tattccgcat gcaggagatg aagcggtcgt tgtaccagat gagaaaaagg ccagagaagt
    31381 tgctttgttt agacagggca gattccgtga tgtgaaattg gccagacgtc aaaaaaccac
    31441 aatagaaggt attatggaaa atatgacggc taccgaatcc aaagtcttga acattgtctt
    31501 gaaagcggac gttcaaggat cactggaagc aatttctgat gcattgacaa aattatcaac
    31561 agatgaagtc aaggtcgagg tgatatctag tggtgtaggt ggaataacag agtctgatgt
    31621 ccatttggct atcgcttcca atgccatttt aatcggattt aatgtaagag cggatggcac
    31681 agcgaagcgt cttgctgaac aagaatctgt atcgattcat tattatagtg ttatttatga
    31741 tatagtcgat cagatcaagg gtgctttgac aggcatgctt gcgccacaat ttaaagaaga
    31801 aattattggt attgctgaag ttcgagacgt attcaagtca ccgaaaattg gtgctattgc
    31861 tggatgcatg gttattgaag gcgttgtcaa gcgtaataat ccaatacgtg tattgcgttc
    31921 aaatgtagtg atttatgaag gtactttaga gtcattaagg cgatttaaag atgacgtatt
    31981 agaagtaaga caaggctttg aatgcggtat cggcgtaaaa aactacaacg atgtgaagcc
    32041 tggggattta attgaggttt ttgagacagt cgaaattaag cgtgacttat agaaaatgag
    32101 taataatttt aagcgaactg acagaattgc tgagatgatt cagcgcaaat tagctttgat
    32161 cattccccag gaaataaaag atccgcgctt gaagggtttt gttacgattt ctgcagttaa
    32221 agtggctgcg gatttaggtc acgccaaaat ttattttact gttcttaatg aagataaatc
    32281 ggtggtgacc aatatattaa atggtgcagc cagttatttg aggagcgctc tggcaagaag
    32341 cattactcta cgaactgtgc cgcaacttca ttttatttat gatgaatcga ttgagtatgg
    32401 acagcggtta agccgattaa ttgatgaagt aaatccccct gattcaaata gtgatgacaa
    32461 caattgaatc tcagtgttcc atagatggaa tcctgctatt aaataaacca caaggaatga
    32521 cctctaacgc tgctcttcag aaagccaaac atttgtttgg tgctaaaaaa gctgggcaca
    32581 caggcagctt ggatccttta gcaaccggca tgttacctct atgctttgga gaagcgacta
    32641 aaatatgcca gtatctgctt aatgcggata aaagctatga aacaattggg aggttaggta
    32701 gtaagacaaa tactgctgat tgtactggcg aagtgatatt ttgcattgaa aattatactg
    32761 ttagccatga ggaaatgatt gcaacgcttg aaaaatataa gggtaaaatc aagcaaattc
    32821 catccatgtt ttctgcttta aagcataagg ggaccccttt gtatcgactt gccagagaag
    32881 ggatagagat agaacgtaag gccagggata tagtaatcag ccaattgaag ttggaacaat
    32941 ttgatggtga atgttttaca ttaacggtat catgcagcaa aggaacctat atacgtaatt
    33001 tagtggaaga tattggtgat acgttgaagg caggagctca tatgacaaaa ttgcataggc
    33061 tctacactgc agggtttgaa aatagtcgaa tgtatactct cgatgaattg cagaatatgt
    33121 ctttattaca aaggcttgct tgcttaatcc ctattgatca ggccattcaa catttaacac
    33181 cagtaattct ttcaggttct gaagtaatag caattcgcca gggcaaggtg atttcaaata
    33241 aaactgatgc tggtgaagga gaagatttgc gtctttatgg tgagcaatcc caatttattg
    33301 gcattggcca agctttaatt catggtgata ttaaagcaaa aaggttagtt tcttttgcgt
    33361 tgtaatgaca atttttgtaa gaattgctgc ctactttgta ataagcatga aattttttac
    33421 acaaatttat atccactact tgtttcattt gacatgcctt ggtagaatgt tcgcctttaa
    33481 aagaatactt agctgagttc caggagtaaa taatgtcgct aaatagcgcg gaaaaagcag
    33541 aaattattaa tcaatacaaa cgcggtgata aagatactgg ttcacctgaa gtgcaagttt
    33601 ctttaattac cggtcgcatt aaatatttaa ctgaccattt taaagaaaat aaaaaagatt
    33661 tccattcgag acgtggcttg caagaattgg ttaacaagcg ccgcaagtta ctaaaatatt
    33721 tgaagcgtaa tgatcaagat cgctatcaaa cattaatcca aaatctggga ttgagagatt
    33781 cctattgatt gatgggatta ttgtctctat ggggacgcat caaataataa atcagtttta
    33841 gaataaaagg cgcagcgttc tgcgcctttt ttttcttata tttacttaat ggggaacatt
    33901 acgtggcaaa aattacaaaa gaaatagtat tcggtaacca taaattaatc ttggaaacag
    33961 gtgaagtagc acgacaggct gatggtgccg taatggcaag tatgaatggt acacaagtgc
    34021 tagtgactgt agtttggaaa aaggatggtg gtgaaagtaa tgattttttc ccattaacag
    34081 tgaattatca agaaaaattc tatgccatag gtaaaattcc tggtggcttt aacaaacgcg
    34141 aaggacgacc atctgataat gaaacattaa tttcgcggct aatagataga ccgatccggc
    34201 cattattccc ggataatttt tttaacgaag tacagataat tgctactgtt ctgtccttaa
    34261 atcccgaggt atctcctgat attatcgcta tgattggagc ctccgctgca ctttcaattt
    34321 ctggtgttcc atttaatggc cctataggcg ctgctcgagt aggctataaa gatggcgttt
    34381 atctgctcaa tccaagtaga aaagaacaag aagaatccaa gcttgatttg gttattgccg
    34441 gcacaaaaga tgccattttg atggtggaat cagaagcgca ggaattaagt gaagacatca
    34501 tgcgtggtgc tatgttatat ggccatgaaa tgatgaaaag tgttataaaa tcaatagaag
    34561 aacttgccag agaagtgggt aagagcaaac ctgagtggaa agctcctgaa attgatacag
    34621 tactaaaagc tagaatcaat gacgtagcaa ggaatgaagt tgaagccgct tatcttatta
    34681 aagacaaaca acaacggtac cagcgattag acgagttaag agagcaaact atttctgctc
    34741 tgctggctga aaatgatgaa ttaaatgcag atattattgc caatatgttt ggtgagttag
    34801 aacgttctat agtacgtaat cgtattcttg acggcgaacc tcgcatcgat ggtcgtgatc
    34861 acagaacagt taggcctatt tctattcgta ccaaattctt ggaaagaact cacggttcct
    34921 gtttgtttac cagaggagaa actcaagcta ttgttgttgc cactttgggt aatgaacgtg
    34981 atgcacagat actagacgga attagtggcg aaaccagaga tcgatttatg cttcattata
    35041 atttccctcc ttattctgtc ggcgaaacag ggcaagtagg tagtcctaaa cggcgtgaaa
    35101 ttgggcatgg ccgtttagcc aagcgtgctt taatggctgt actgcctgat actaatgagt
    35161 ttccttatgt gctacgtatt gtatctgaga ttactgaatc caatggctcc agttctatgg
    35221 ctactgtttg tggaacaagc ttggcattga tggatgccgg ggtaccttta aaagcaccag
    35281 tcgctggtgt ggccatgggt ttaatcaagg aaggtgaccg ttatgctgta ttaacagaca
    35341 tattgggtga tgaggatcat ttaggtgata tggactttaa agtggctggt acagaaaaag
    35401 ggattacagc tttgcagatg gatatcaaga tttccggtat taccaatgaa attatggaac
    35461 aggctctgga gcaagcattg gagggtcgta cccatattct tggtgtcatg aacaatgccc
    35521 ttgcagaaca tagaaccgag ttatctcagc atgctccaag aatcacaacc atgaaagttg
    35581 ctgaagataa aatccgcaca attattggca aaggtggggc aacaattaaa gggcttattg
    35641 aaagtactgg cgtatctata gatattgatg actccggggt tatccaatta ttttctccgg
    35701 ataagatggc tctggaagag gcgcaaaaac aaattaaagc tttaattgct gaaattgaag
    35761 tgggtcagac ttatcaaggg aaagtaagca aaatagttga ctttggagct tttatcaatt
    35821 tactgcctgg taaagatggc ttactgcata tttcacaaat atgtgccgac agaacacaaa
    35881 aggtagaaga ggtattgcag gaaggtcagg aaattgaggt ctttgttgct gggatagata
    35941 agcaagggcg agtgaaactg gagtggaagg ataagccgca agctgaagcc aaagaggtcg
    36001 aagacgcccc ggtatctgcc acgtttctta caatggaaga gcaatctgaa gaaatcaatt
    36061 caggcaacaa aatttctgaa gaagaggaat aataaggtag tagtatttta agttctattt
    36121 attatacact cgtggaaagc aagagctttt cgcgagtttt aactgaatgc ttgcaattgg
    36181 tgtatatgac cgcataaatt ataagctaat aaaatagagt ttaagcatga agctattctc
    36241 ttttttcttg tattgataag ctcagctagt ttaggtcagg atttaccatt acatctcctg
    36301 aagtacccgt ccggattttc agtaagtatc tatgcagcct ccgtttctga tgctcgagaa
    36361 atggcactgg gcagtaaaga tatagttttt gtaggttcaa gaaatgccgg gaaagtgtat
    36421 gctgttattc cagataaaaa aaactctcat ggtactcgag ttttaaccat agcttctgga
    36481 ttgaatatgc caaatggtgt tgcctttcat aaaggtgcac tttatgtcgc cgagattggg
    36541 cgcattttac gctttgataa tattgaatca aatctgaata atcctcctga accagttgta
    36601 gttactacaa aattaccgaa ggaaaaagct catggctggc gttttattag ttttggacca
    36661 gatgataagc tttatatagg aattggcgcc ccttgcaatg tttgcttaat ggaagatacg
    36721 cgctatgcca ctattatgag aatgaatgcc gatgggcaag atctcgagat ctatgctaaa
    36781 ggaatacgta atacagtggg gtttgattgg gatccctttc atcataatct ctggtttaca
    36841 gacaatgggc gtgactggct aggtgatgac ttacctccag atgagctcaa tgtagctccg
    36901 agaaaaggca tggattttgg ttttccttat tgctatggta aaagcacagc agatcctgtt
    36961 tatggcaaaa tacattcttg ttccgagttt actccggctg tttttgaatt cccagcgcat
    37021 gttgctcctc ttggtatgtc attttataca ggcaaattgt tcccttccaa ttattgggga
    37081 caaattttta ttagtgagca cggttcctgg aatcgtagtc ataagatagg gtatcaaatt
    37141 actatagtaa aaattaagaa taatcacgta actgaggtta aaccatttat cagcgggtgg
    37201 ttacaggggg aaaaagcatg gggaagacct gtcgataccc tcgttatgcc agatgggtca
    37261 ctattaatct ctgacgatta tgctaatgta atatatcggg ttggctatca gcagtaaatg
    37321 gagcaattat ttgtattata ggcggggata aagcccctac ctatttctta tcagtattaa
    37381 cgaattaaat ttaagaattc caaacggcaa ctcgaattgt tacgcatttc accgagcata
    37441 acggaagttg tcatgacaga attttgtttt tcaactccac gcatcatcat gcaaagatgt
    37501 tttgcttcaa ttactacggc gacaccgcga gctccggtag tggcttcaat acagtgagca
    37561 atttcgcccg tcagccgttc ttgaatttgt agtctcctcg cataaaaatc aacaatacga
    37621 gcaattttag ataaacctat tactttacca tcaggtagat atccaacatg gcatttaccc
    37681 aaaaaaggca ataagtgatg ttcgcataat gagtacagtt ctatgtcctt gacgataacc
    37741 atttcactca tgtctgaatc aaacagagca ccattaatga tttcatcaag gttctcattg
    37801 taacctttag ttaaataacg taaggcatta gctgctcgtc caggagtatc ctttaatcct
    37861 tcccgattta tatcttcgcc taattcttta agcaaatttg agtataattt ttccatagca
    37921 agaattccta acctggcggc caagtcattt gacgcccgcc aagtaaatgt aaatgaatgt
    37981 ggtaaacttc ctgcccgcca ccagaattaa cattaaaaac taatcgataa cccatttcac
    38041 ttagtccttc ggcctgcgct aatttttttg ctcttattag aatattagca agaagttcgc
    38101 tatcatcatc gttcacatca ttgatagttg caatatgctt tttgggtata atcaataaat
    38161 gtgttggagc ttgaggtcta atatcgcgaa atgccaaaat atttttgtct tcaaaaacta
    38221 ctgttgcagg aatttcaccc tgtgcaattt tgcaaaacaa acaattcata ggtcatccga
    38281 tgttttagat tcaaaatgtt attatacatt tcatttatta tattgcgaac gcttgtcttt
    38341 gccttttgat gcgatttttc gcataatatt acttttattt attattaagg gtgcgtaaat
    38401 gagtctaatg gaaattcaga gcggtcgtga tgttccaaat gaagtgaatg tgatcataga
    38461 aatccctatg cacggagagc ctgtaaagta tgaagttgat aaaaaaactg gtgctttatt
    38521 tgtagatcga tttatgacga cagcaatgtt ttatccgacg aattacggtt atattccgaa
    38581 tactctttca gaagatgggg atcctgtgga tgtgttggta ataactcctg tccctttgat
    38641 cagtggggct gttatttcat gtcgagctgt tggcatgtta aagatgacag atgaatccgg
    38701 agttgatgca aagattttgg ctgttccaac aaccaaacta agtaaaatgt atcaatccat
    38761 gcaaacttat caagatattc ctcagcattt attgctatct atcgaacatt tttttaagca
    38821 ctataaagac ttggaagaag gtaaatgggt taaggtcgag ggctgggtag gcccagatgc
    38881 agctcgtgag gaaataacat ccagtattaa ccgttataat cacacaaaaa agtaaaacag
    38941 tgatttgaac tgtgccaggt gttgggcatt gctcagcacc aacctgatat ctttagctat
    39001 aatagcggca tcatctcagc ccctactaca aaacagcgct ttctattgta taatttgtca
    39061 ttcttaaaag tataaggata ttcagtgcaa cttcctcttt ctactttatt ggttttattg
    39121 tttttcctaa tttgcttgtc tgcttttttt tcaggttccg aaattggcat gatgtctatc
    39181 aatagatata aattgcgaca cttggtaaaa agcaatgata agcaagccat tcgggttaat
    39241 tccatgctgg ctcggcctga taagctacta agtgtggttt taattgggaa tactttggca
    39301 aatatagtgg catcaactct cgccacttta atcggacagc ggttttatgg ggatgcaggg
    39361 gtggctatag caactgcctt gttgaccatg ctgatcctgg tttttgcaga aatgactccg
    39421 aaaaccctcg cggctattta ccctcaacaa gtagcttttg catcctcatt accgttgagc
    39481 attttacaat ggattttttc tcctttggtt catttaatca gtttgatttc caatggtatc
    39541 ttaagattat ttaatatatc ggtaaaacgc atgcaaaagg agatgctaac tggtgaagag
    39601 ttgcgatcag tggttcatga ggcgggggga ttaatgccgg tagagcataa aagtatgctg
    39661 attagtttat tggatctgga acaagcaact gttgaagaca tcatggttcc caaagccgat
    39721 attgttggga ttgatttgga tttaccctgg atagaacttt tagatcaatt ggaaacagca
    39781 caacacacaa gattaccaat ttatcatggt tctattgatc atttggtagg gatgatccac
    39841 gtaagaaata ttttaaattt agcattagaa aataagctgg atatggaaaa tttgttaaat
    39901 agtgctgaag ttccctattt tattccagag gctaccccat taaatataca aatccttaat
    39961 ttcaggaaaa tgaaaagaag aagttgcttt gttgtggatg aatatggcga tattcttggg
    40021 cttgtaacta tggaagatat tcttgaagaa atagtaggtg aattcacaac agatatcgct
    40081 gatctgagtc gtgatatcac ccctcagcaa gatgggtcgg tcatagttga tgccagcata
    40141 actattcgcc atttaaaccg catgatgggc tggagcctgc ctttaatcgg tccaagaacc
    40201 ttaagtggcc tgattattga gcatttaggg tatattccac cccctgactc ttgtctggtt
    40261 attgatcatt atcatattga aatattaagg gtcagcgata atatgataag aggcgtaaag
    40321 atgattaagg ctaataaaaa aagaaaatag ctcttataga ttctgatgct caattgctct
    40381 gtcattatca aagatagagt tcgattttat cttccaaact attgtggttg actcataatg
    40441 ttctcaatag gcttcaagct tgcccttatt ttggtttact taaatagtta ttgattgatg
    40501 aagcgataca atttattagt tttttcacaa tagactactt cgatgcatat atttaagatt
    40561 tgattttga
//
LOCUS       DASLOE010000012        38415 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.012, whole genome shotgun sequence.
ACCESSION   DASLOE010000012 DASLOE010000000
VERSION     DASLOE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 38415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38415
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.012"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            75..503
                     /locus_tag="U6309_000486"
     CDS             75..503
                     /locus_tag="U6309_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HEN5503941.1"
                     /translation="MMHLQELAKHGQQEKEVILNERLPSFVSPGCHLHASYHIETKDD
                     FYLIHLRVQGNLNSICQRCMENFTVCYNNSTVIAVCRSDERAEQLLSQYECLVSSNWQ
                     VDLNELIIDELHLYAPQVHPDINECANEINEILTGKKQTS"
     gene            589..780
                     /gene="rpmF"
                     /locus_tag="U6309_000487"
     CDS             589..780
                     /gene="rpmF"
                     /locus_tag="U6309_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN5503942.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTKPTLSVDPTTGETHLRHHMTP
                     DGYYRGKKIIDAETAYEQE"
     gene            786..1814
                     /gene="plsX"
                     /locus_tag="U6309_000488"
     CDS             786..1814
                     /gene="plsX"
                     /locus_tag="U6309_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEN5503943.1"
                     /translation="MKNITIAIDAMGGDHGLEIVIPACIRAVKNNPDLKLLLVGVQDK
                     ISASLKKHGMLSCQQFTIVHASEVVTMDELPSHALRNKKDSSMRIAINLVKEGRAQAC
                     VSAGNTGALMATARYVLKTLPGIDRPAIVSELPTMGGKTRVIDLGANVDSCAEHLFQF
                     AVMGSALIQAIENKPKPKIGLLNIGVEEIKGNDQVKRTAHMLAECSVMNYVGYVEGDH
                     FYSGDVDLVVCDGFVGNVALKASEGLAKLLLTVLKESFSRNWLTKIAGLIALPALKHL
                     KNRLDPSRYNGASLLGLNGIVVKSHGGANEVGFQHAIEQAVLEVKNNVVDLVRDQIND
                     FINQGLLL"
     gene            1811..2764
                     /locus_tag="U6309_000489"
     CDS             1811..2764
                     /locus_tag="U6309_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEN5503944.1"
                     /translation="MKNAVINGTGSYSPERQMTNAELETMLDTSDEWIVTRTGISSRS
                     VAQEHETTSYMASRAAEQALEASGLDAEEIDLILVATCTPDYFFPSVACHVQHALGIK
                     RPIPAFDIGAACSGFVYAMDVAKQYIATGAAKHVLVVGSESMSRAVDWTDRSICVLFG
                     DGAGAVVLSASDRQGIMGSVLHSAYDSDKLLVLRNSTFEQDRATIGMRGNEVFKIAVN
                     IMGNIVDEVLEASHLKKSDIDWLIPHQANIRIIQAIAKKLSLPMSHVIVTIGNQGNTS
                     AASIPLALDYSIKNNRIKRDEILLIESFGGGMTWGAMVIRY"
     gene            2791..3738
                     /gene="fabD"
                     /locus_tag="U6309_000490"
     CDS             2791..3738
                     /gene="fabD"
                     /locus_tag="U6309_000490"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN5503945.1"
                     /translation="MVKSAFVFPGQGSQSIGMLSDFMPQYTVVTDAFAEASDALGYDL
                     WDLVQNGPETKLNQTEYTQAAMLTADVAIYRLLMQLGVPQPQVMAGHSLGEYAALVCA
                     NSLSLKDAAQLVARRGQVMQNAIPLGEGAMAAIVGLSDEQVRSLCEQASTANHLVTPA
                     NYNALGQIVVAGHRVAVDKLIQLAEEAGARLAMIIPVSVPCHCPLLKNAAELFAENLA
                     QIEFQVPSVDVISNVDLSIYHSTQHIRDKLKEQLYSPVRWVETVQLIQQRGIELVVEC
                     GPGKVLNGLIKRIERNLTTISVYDTISLDQVIERLHIPV"
     gene            3750..4496
                     /gene="fabG"
                     /locus_tag="U6309_000491"
     CDS             3750..4496
                     /gene="fabG"
                     /locus_tag="U6309_000491"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEN5503946.1"
                     /translation="MINLEGKIALVTGASRGIGRAVAEKLARNGAFVFGTATTEQGAA
                     SINSFFEKEKLAGKGIVLDVTSTEQVENLMSTLSDNDQCPSILVNNAGITADNLLLRM
                     DDEEWYKVIETNLNSIYRMTKACLKPMFRARWGRIISIGSVVGASGNSGQVNYTAAKA
                     GVIGFSKSLAQEIGSRGITVNVVAPGFIDTDMTTALPDMVKEEMLKRIPMRKLGQPED
                     VADAVAFLASDSAKYITGETIHVNGGMYMS"
     gene            4581..4829
                     /gene="acpP"
                     /locus_tag="U6309_000492"
     CDS             4581..4829
                     /gene="acpP"
                     /locus_tag="U6309_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN5503947.1"
                     /translation="MSTVEERVRKIVVEQLGVKEEELKNDASFVDDLGADSLDTVELV
                     MALEEEFETEIPDEKAEKITTIQEAIDYIESNLNKEEA"
     gene            4849..6087
                     /gene="fabF"
                     /locus_tag="U6309_000493"
     CDS             4849..6087
                     /gene="fabF"
                     /locus_tag="U6309_000493"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEN5503948.1"
                     /translation="MNSRRVVITGMGMLTPVGLNVEETWRSILAGVSGVGLVEDFDTT
                     EYPTKIWAKVKNFNVENHMPIKDARKMDVFTQYGMAAADEAMLDSGLKIDEILSRRIG
                     VAVGAGIGGIQTITNNQDKLVAGGPRKVSPFFIPAGIINMVAGQISIKHNLKGPNISV
                     VTACTTGTHNIGLAGRIIAYGDADVMICGGAEMTTTPLCLAGFSAVRSLSKRNDEPEK
                     ASRPWDKDRDGFVMGEGAGILVLEEYEHAKARGAKIYAELVGFGMSGDAYHITAPDED
                     ADGASRAMEAAIQDAGIDPRQVDYINAHGTSTYLNDLNETKAIKRVFQNHAYDLAVSS
                     TKSMTGHLLGAAGAVEAIISILAIRDQVAPPTINLDNPDEGCDLNYVAHVPQSRTINY
                     VLSNSLGFGGTNGSLLFKRI"
     gene            6088..7086
                     /gene="mltG"
                     /locus_tag="U6309_000494"
     CDS             6088..7086
                     /gene="mltG"
                     /locus_tag="U6309_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEN5503949.1"
                     /translation="MQFSRHKKLVILVLSLFFMSFLFLFWNIYHLLYRPIIPKNASPM
                     IIALDKATSAYQFAYILQDKKLIHSAKFFLLIIRFEGLSHQLKAGVYQITPGETAMKL
                     LHRVVAGDVITQNFTIIEGSTQQKVDYDLRQANYLKYNPEDWAIVKENYPSAEGLLLA
                     DTYQYQGGSNSRALLEQAHRNLLNYLNTSWTNRAPNLPYKTAYELLIAASIIEKETAI
                     AQEKKLISGVMVNRLKKKMPLQMDPTVIYGLGNQYKGKLTHNDLLIQSPYNSYLNRGL
                     PPTPIAMVGKEAIDAAAHPQLSNYLYFVAKGDGTHQFSETYEQQRQAINQYRRKDY"
     gene            7086..7724
                     /locus_tag="U6309_000495"
     CDS             7086..7724
                     /locus_tag="U6309_000495"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEN5503950.1"
                     /translation="MSSLTGKLIVIEGLEGAGKSTAVNLVVELLSQKKISTITTREPG
                     GTRIGEILRSIIKNPEYNNVLDDRSELLLLYAARIQLIEQVIKPALNEGQWVIADRFE
                     LSTLAYQGGGRKMDMRVINELSNFCLKGFKPDLTLYLDINPELGMIRAKSRGKFDRIE
                     QESIEFFHRIHDTYHILVKQNPEIMMIDANRSLEEVQSSIQSVIEEFIEHNI"
     gene            7721..8626
                     /locus_tag="U6309_000496"
     CDS             7721..8626
                     /locus_tag="U6309_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEN5503951.1"
                     /translation="MITYQEQWQKMQMALAVRRIPQAMLFLGSLHCGLIEFTNQVIKL
                     LLCKIKQNEPCLTCMDCQMIERFEHPDVEWIKPERSGGIIKIDQIRGLQSTAFLTPQR
                     TNHKLIIIESAESMNIAASNALLKILEEPAQHTIFILLAQQIGNIPPTVLSRCQMTQF
                     AVFDELPCNNLLALGEYYTQESNSTMVFKHSESILEGLIAIIERREHPCTLASQWIQH
                     ELSAFLWFLYLLYSQLQYMHLFRVAYSGTLTKQLNKLSSLLNPVMIFAQVDKINTLLR
                     KLSHNMNMNHTLVLEDLLFSLVVDY"
     gene            8753..9094
                     /locus_tag="U6309_000497"
     CDS             8753..9094
                     /locus_tag="U6309_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEN5503952.1"
                     /translation="MTEKPQLINCSFPNEASLYMAYMPFVKGGGLFIRTQHVYSLGQS
                     LELSVKLLNENEPYLVTGKIVWITPKGAQGNKPPGVGIQFTGENSRYLCNKIETYLAG
                     MLKSCQITDTI"
     gene            9150..9938
                     /locus_tag="U6309_000498"
     CDS             9150..9938
                     /locus_tag="U6309_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN5503953.1"
                     /translation="MLIDSHCHLNFIDLTHFNHDLAQVINQARENGVEHFLSVCVELS
                     DYPQLERLAKDYPDISISVGVHPNSEMNYPVTAQILCELAQNPACIAIGETGLDYYRN
                     PTEEAQSLQRDRFREHIKAALITAKPLIIHTRQAAEDTLLMMAKEGASQIGGVMHCFA
                     EDLDIAQRAIDLNFYISFSGIVTFKNAVSLQDVARQIPLEKILIETDSPYLAPVPHRG
                     KQNHPALVKHVAETIAQLRGMDFQEIAEITTNNFYNCFNIKRQG"
     gene            10050..11150
                     /locus_tag="U6309_000499"
     CDS             10050..11150
                     /locus_tag="U6309_000499"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEN5503954.1"
                     /translation="MSLYIGLMSGTSMDGIDAALLELPSNQLIHGITKQYSDDVRRNL
                     DDLIMGNHLTLASICQLNTLIGREFAEAVHQLLIEIKVHPKEIQAIGSHGQTVCHDTS
                     GNIPYTLQLGCGHTISSLTGITVVADFRTRDLVNGGQGAPFAPLYHQQIFSKVNESVA
                     VVNIGGIANVTFIAKNQMTRGWDIGPGNCLMDAWIYKNKGVLFDKSGVWASQGEVIHP
                     LLEYLLQDPFFHLDSPKSIGKEYFSLSWLQKHLKPDYTPADIQATLLALTAHTIAETI
                     LNESGEIKQLYLCGGGAHNTHLKENLARLLPGITVKSIAELGISPDYLEAMMFAWLAA
                     QTINQIPVNLTSITGAKGIAILGAVYPIIKSY"
     gene            11206..12498
                     /locus_tag="U6309_000500"
     CDS             11206..12498
                     /locus_tag="U6309_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5503955.1"
                     /translation="MTAHYNVASNSGARNTLATSYFIFFLAASFYLYEFILQVAPSVM
                     AESMMKTFGVTGQGFGVISAFYFYAYAPTQLPAGVLYDRYGPRKLMTFAIVLCAFGSA
                     FFASTDSVFTACIGRFLIGIGSAFSFIGVLVLISRWFPPYYFAILAGVAQLMSSVGAM
                     FGEMPLAALIDLVGWRSASFILAGVGFLLAILFWVFIRDYPHQQNQTIPDHYLRDEWK
                     RLVAVCKHAYTWIIGGYAFAIWTPIAVFAALWGVPFLQEKFQVSVVAASGLCSMIWLG
                     IGVGSPLLGWLSDKLESRRIALIISAVLGLIATLIILYWSELSYNWAYVVLFVLGLGA
                     GGQTVSFAVVKENNTPELVGTASGFNNLSVLIGGAIFQPLVGYILQQTGSWRIVNGAH
                     VYSISSYQTALLVMPICFLASLLIATFLLKESHPARRD"
     gene            12571..12714
                     /locus_tag="U6309_000501"
     CDS             12571..12714
                     /locus_tag="U6309_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503956.1"
                     /translation="MGKDYIGASRYPESIQAEEMRKHRLEALARDIKFHTRLRDGINA
                     SSA"
     gene            complement(13015..14295)
                     /locus_tag="U6309_000502"
     CDS             complement(13015..14295)
                     /locus_tag="U6309_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5503957.1"
                     /translation="MSEPLIKISYRQAIIFACFLVLYEFLTYIANDMIMPGMINVVKS
                     FNAHESVVATSLTVYVLGGASLQLILGPLSDAYGRRPMMLIGSCLFFLFTLLIASSHS
                     MNQFLIARFFQGMGLCFIGVIGYATIQEIFEEMDAIRLIAIMANAAILAPLLGPLLGA
                     IIIHYASWRLIFIIIALGALLAYWGLWRFMPEPIGQVKRDGQLIPKTPFALNAITRNY
                     KTLLSNQAFCYSAIAEGLVGIPCIAWIALAPIILIAEAKLTVIQYGLWQLPIFGATIL
                     GNWCLHHLTYKYKIERIIFIGCIIMVVGLALTALLPYFYGNNYVYLIPGIIIYFFSLS
                     VINAPLNRYCLFVTAVSKGTASALISLSIMIIGAIGIEIANLFYQHHNNLHFALYCNA
                     VGLLFLIFIGLTFFVGTPKKSEIHDEANSNVANS"
     gene            14526..15692
                     /locus_tag="U6309_000503"
     CDS             14526..15692
                     /locus_tag="U6309_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN5503958.1"
                     /translation="MPKEKVVIIIPTYNEEDNISNTIDLILKETYSLADYQVDILVFD
                     SHSTDSTAHIVRELTYTSQRVHFAQEPQKTGLGSAYLQAMRIAINELNADVVFEFDAD
                     GSHQPCYIAPMLELLKQHDVVVGSRYISGGRIPSNWAFHRKLLSVGGNWVARMLLTGK
                     YKDLTSGLRAIRTNLLKKLLPSNFLSNNYAYKLELYWLLHKAKARIVEFPIQFIDREK
                     GKSKLPKNSMIDTLRVLFTLRFRELRQYLTMCCVGSIGALIQFSLYNWLRSSNYSALV
                     SSQVAITAAIISNFIFHHSITFKQKTDFRITKTTFSRFTQFIIYSTFMVYFQSLWVHI
                     FVHSFGQGVLKENIIVASGLGIGSIINYLFYSNIIWSSKGAHSLNSNSSKETQI"
     gene            complement(15781..17166)
                     /locus_tag="U6309_000504"
     CDS             complement(15781..17166)
                     /locus_tag="U6309_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEN5503959.1"
                     /translation="MFLHMNRYISLYFLLILLIAPIHLLGLDSYYYWDWSRHLALSYY
                     DGSPMIAYFIRIATLLFGDTLFALNLVTIVSTALTSFIIYKTARLCLNKEASYVTVLI
                     WLFSPLVTLDLINQTTYDTPLTLFWALTLYFTIRYIQFNRNQDLYYIGLSIGLMLLSK
                     YSGIVLVLALLIFLVATPYRRLFKSRHFYLALTLAIIIFSPVILWNAQHDWVSIRYQL
                     STHQTQNTHHAMGSVLHAFFSIFLPAINFLFIPPALYLSKGTPRKSPVFYLCLIICVT
                     FGLFYLFVAGKATIRGYWLSQYIITASLLAGYCYQEWAYRNSTYLLIVFYTLTSGIIL
                     MDNTTLFSFGYSKKLAYYQSIQQINQSNDKLPSIVITPGWFEARMLFFLKNKPTIFTF
                     DCGSMQNQYGYWSPNLNTLGEVLYIDTFDRLSCVKKYFAQCQSLNLPATFDGKGKPII
                     YAYWCSNKLDT"
     gene            17265..18407
                     /locus_tag="U6309_000505"
     CDS             17265..18407
                     /locus_tag="U6309_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEN5503960.1"
                     /translation="MKLYFFGDDNNKPKLSEHLIIYILSLLDPKSQSVASLVNKDWSR
                     LVQLTRQYLSMLPVIHRIPLFANLGLDVLKLKLMTGGMTNSTFRLRIKNAPEKWVMRI
                     PGIGSSAFITRRDEAHNAKQAEKLGLNVPIAFFDPEDGLQVTRFIEGVHSLDDKALAR
                     KEILHEVASMMRRLHKSPPFDNNTLFFERNEELLDLLKRKPFNFPGEIEFIEQQMKNL
                     KEIFSSYRIQQFPCHNDTTPLNFILSENPEIKGSEKIHQIDWEYSSNNDFIWDLVYFM
                     VEAKLNREQQLILLKAYFNTEELSGSLLAWIEVYKPIIEWWITIWSWTQLANGANAVD
                     LSSYEQLGLERYHNTLSHLKSEGYVKALALIETDSHSLTFTGQRPF"
     gene            complement(18460..19230)
                     /locus_tag="U6309_000506"
     CDS             complement(18460..19230)
                     /locus_tag="U6309_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEN5503961.1"
                     /translation="MTILVFDIETIPDIESGRKLFALDGLSDEDTACAMFALRREKAG
                     NDFLPHYLQKICAISVVISHGNQIKVWSLGDENSDEKELITRFFAGIDKHTPTLVSWN
                     GSGFDLPVLHYRSLYHSISAPTYWETGENQQTFKWNNYLSRFHYRHIDLMDVLAGYQN
                     RAFAPLDDISTMLGFPGKMGMSGSKVWEQYQAGQLKEIRDYCETDVLNTYCVYLRFEL
                     MRGTISQEGYDRNIAQLKNYLGAEKDKMHLQEFLKKML"
     gene            19476..20072
                     /locus_tag="U6309_000507"
     CDS             19476..20072
                     /locus_tag="U6309_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503962.1"
                     /translation="MKIRFTLLCFSIFLTGCVSKPPADINNICNIFKQYPKWYVDSKD
                     VERRWKVPVPVQMAIIHQESKFNARARPPRTKLLWIIPWTRPSTAYGYTQALRSTWDN
                     YKRTNGWFWSSRDDFGDGVDFIGWYANQANIKAGIPRTDTYSLYLAYHEGIGGYQRKT
                     YLKKPWLISVARKVKAKSQLYAMQLNSCQGSLKSRSWW"
     gene            20467..21123
                     /gene="adk"
                     /locus_tag="U6309_000508"
     CDS             20467..21123
                     /gene="adk"
                     /locus_tag="U6309_000508"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEN5503963.1"
                     /translation="MRLMLLGGPGAGKGTQASLLINRYKIPQISTGDMLRAAIAKGTP
                     LGLSAQKIMESGGLVSDDIIIGLVKERLKNPDCDRGFLFDGFPRTLVQAEALKDAEIH
                     LDHVIEIAVDDEEIIERISGRRIHQPSGRVYHVVNQPPKNPGVDDITGEPLIQRDDDK
                     EETIRKRLQVYHSQTAPLVQYYKEWAESGSKEAPKFHTISGTGTVEQIFDNIVTILET
                     "
     gene            21132..21494
                     /locus_tag="U6309_000509"
     CDS             21132..21494
                     /locus_tag="U6309_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN5503964.1"
                     /translation="MPTQTINSAEFESLINDNEIVFIDFWAEWCAPCKQFSKVYEQVS
                     DQFPAIKFVKVNIEEQQELADFFEIRSIPHLMVFKDSIVIYSDAGSIPESTLKELAQQ
                     ALDADVSEIRAQLEQKDK"
     gene            complement(21454..22974)
                     /gene="glpD"
                     /locus_tag="U6309_000510"
     CDS             complement(21454..22974)
                     /gene="glpD"
                     /locus_tag="U6309_000510"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEN5503965.1"
                     /translation="MDQVFDVAIIGGGINGCGCAADAALRGLSVILFEQDDLASKTSS
                     SSTKLIHGGLRYLEHYEFGLVKKALQERQTLLNLAPHLVHSQSFVLPYLKHMRPSWLL
                     RLGLFFYDHLSRKNHLPKSRSIHRNNKNNYFTPLKEELNRGFLFYDATTDDARLTILN
                     AIQAKNHGASIRPHAKVTHAEVINNIWHLTIQPTKGQSYKAYAKSIINATGPWVQSIA
                     QLTHTPIQKDITLVKGSHIIVPKLFEGNHAYFLQHNDQRVIFVIPYHGFSMIGTTDIH
                     YTGDLNHIEIGEEEIDYLIELVNAYFKFKITKEDVVHSWSGIRPLLADDSKEVKSLSR
                     DYSYEFNHYPAPIITIFGGKITTYRQLAEEVVDQLTPVFPQMKPSKTQHTPLPGATLA
                     DMSFTQYVNYAKTKYHWLDDELLNRYLNTYGCYTEIFLSQCNNIEAMGKKYGPSLYQV
                     EVDYLILEEWANYCDDILNRRTKLGLTMDNVSKKELADYLSSISTYPSAQAELVFH"
     gene            23036..24511
                     /gene="glpK"
                     /locus_tag="U6309_000511"
     CDS             23036..24511
                     /gene="glpK"
                     /locus_tag="U6309_000511"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEN5503966.1"
                     /translation="MNYILAIDQGTSSTRAMIYTLQGDLIANSQYPLTQYYPNLGWVE
                     HDPEEIWQKTLNAMRDVVSQVQGKAILCCGLTNQRETTLIWNKKTGECLAPAIVWQDR
                     RTEEFCYSLSEYSSMIQEKTGLLPDPYFSASKLNWLLKNNAQAQSLINQGDLAFGTID
                     SFLIWRMTKGASHATDITNASRTMLFNIHDEKWDKQLLELFSISESILPEVYSSDNHF
                     GVIDKEWLGVEIPITGVAGDQQAALIGQGCFNGGMVKATFGTGGFLLLNTGDKPVFSK
                     HKLLTTIAYKIKEKTAYGLEGSIYHAGTTVKWLRDEMKLISHSAETEVLAQSLDSNNG
                     VYLVSSFTGLGAPHWLSITGTAIIGVSRSTNRAHFARAALEGVCYQTRDVLTCMRDDS
                     QLDLTLLRADGGMAVNQWFLQFMADQCQLTIQKPKDIETTAKGAAVLAAIGSGLFDSV
                     SSVNKVWDVENEFQPSREREVVEKDFRGWLEALAMVKARHA"
     gene            complement(24605..25876)
                     /gene="gltA"
                     /locus_tag="U6309_000512"
     CDS             complement(24605..25876)
                     /gene="gltA"
                     /locus_tag="U6309_000512"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEN5503967.1"
                     /translation="MTQKTAKLSIDGQETIDLPIYTPTLGNDVIDITQLGSHGVFTFD
                     QGFLSTASCESKITYIDGDKGILLYRGYPIEQLAEKKDFLDVSYLLLNGELPNKEEKK
                     AFVSLINNHTMVHQQMYQFLNGFRRDAHPMAIMVGIVGALSAFYHESMDLNNAQDRFI
                     SAIRLVAKMPTLAAMSYKYSIGMPYMYPKNKMSYAENFLHMMFGVPSEDTTPDPVLVR
                     AMDTIFILHADHEQNASTSTVRLAGSTGANPFACISAGIGALWGPAHGGANEACLNML
                     KEIGDVSRIDHYIKRAKDKNDPFRLMGFGHRVYKSYDPRAKVMRKTCYDVLEAVGAKE
                     APLFKLAMELERIALEDEYFIEKKLYPNVDFYSGLTLSAIGIPTNMFTVIFALARTVG
                     WMSHWMEMVATKSRIGRPRQLYTGEKIRDVK"
     gene            26102..26983
                     /locus_tag="U6309_000513"
     CDS             26102..26983
                     /locus_tag="U6309_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEN5503968.1"
                     /translation="MITPAELPINHRGAVYHLDLRTEEIADLIITVGDPGRVSLVSQH
                     FDKIEVQRSHREFVTHTGYLGTNRITVLSTGIGMPNIDIVMNELDALANIDLSTRTVN
                     TNKKSLTIIRLGTTGGVTANCMPGDIAISRYAIGFDSLIDYYQYQPMGVLETIREELS
                     QYLMGESGPFYISESCPQLAEQFSALGHTGITATCCGFYGPQGRQLRLPLHYPNLITQ
                     LGKFCTQNYSVINLEMETAAIYALGSMLGHKCLSLSVVINNRIHGSFVTDVKSCLDSL
                     ITKSLANIKNILEPYEA"
     gene            27067..29658
                     /gene="gyrA"
                     /locus_tag="U6309_000514"
     CDS             27067..29658
                     /gene="gyrA"
                     /locus_tag="U6309_000514"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEN5503969.1"
                     /translation="MVYLAKEVLPVNIEDELKQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMSELGNDWNKPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSMRYLL
                     IDGQGNFGSVDGDAPAAMRYTEVRMSKVAHALLADLDKETVDFSPNYDETEFAPVVLP
                     SRIPNLLVNGSSGIAVGMATNIPPHNLTEVINACIALVDEPDTSLEDLMEIIPGPDFP
                     TAAIINGRAGIIEGYRTGKGRVVIRARTEIETDESSGRQSIIIQELPYQVNKARLIER
                     IAELVRDKKVEGISGLRDESDKQGMRVVIELKRNEVADVVLNNLFAHTQMQNVFGINM
                     VALVDGQPRTLNLKQILEYFIKHRREVVTRRTIFELKKARSRAHLLEGLGIALANIDE
                     MIALIKQSPTPQDAKSALLSKIWQPGLVKAMLEKAGSNASRPDDLTEEYGLHENGYKL
                     SEAQAQAILELRLHRLTALEQDKIINEFEELLNLIKELLDILASPERLMQVIRDELIE
                     IKSQFGDERRTEITASQEDLTIEDLITEEDVVVTLSHQGYVKYQPITAYQAQRRGGKG
                     KSATHVKDEDFVERLVIASTHDTLLCFSNHGKLYWLKAYQLPQASRASRGRPIINILP
                     LAEGEEINAMLPVREYKDGSYVFMATKKGTVKKVPLNAFSRPRSNGIIAVDLEEDDSL
                     VGVDITDGTRDIMLFTDAGKVIRFDENKVRPMGRTARGVRGIRVEKDQAVKSLVVVDP
                     NGGTILTATENGYGKRTHIDEYRVSGRGGQGVISIQVTERNGKVVRSLQVTDNDEAML
                     ITDKGTLVRFKVNELSVIGRNTQGVRLINVSSGETVVGMQKIVDLGEELEEAEDSSLN
                     AEDNSDE"
     gene            29651..30739
                     /gene="serC"
                     /locus_tag="U6309_000515"
     CDS             29651..30739
                     /gene="serC"
                     /locus_tag="U6309_000515"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEN5503970.1"
                     /translation="MNSRVFNFGAGPAMLPEEILKEAQEEFLNWRNTGMSILEIGHRT
                     PEIINLLSTAEQSLRELLNIPKNYHVLFLGGAARAQFAMIPMNLLQPGDEAAYFITGI
                     WSKMAYHEANLLKQAYYLSNEEKEGFVSIPDYQKWELKSNTAYVYYTPNETINGVRFP
                     YVPKTGGVPLVADMTSCLLSEPININQYGLIFAGAQKNIANAGLTVVIIHEDLLKNQP
                     EPVVPTMLNYKNHAEHRSLYATPPVFNCYLASKMFEWIKTQGGIEGLFQRNCLKAAKL
                     YQYLDSTDFYLTPVSKEARSIMNICFSLCYPDLEQKFLDMANERGLKALKGHRFAGGL
                     RASLYNAMPMAGVDALIEFLSEFAKENG"
     gene            30745..32046
                     /gene="aroA"
                     /locus_tag="U6309_000516"
     CDS             30745..32046
                     /gene="aroA"
                     /locus_tag="U6309_000516"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN5503971.1"
                     /translation="MLNFISKPVVCLKGEITVPGDKSISHRSIIFGAIAIGTSVIDGF
                     LDGEDCIATLKAFQSMGVRIEGPDKQCVIIHGVGKYGLKQPQNIIDCGNSGTSMRLLA
                     GLLAAQQFDSQLTGDESLLKRPMLRVSRPLSQMGADVTTQDGKPPIVIKGGKKLNGIH
                     YVMPEASAQVKSCLLLAGMYAEGQTKITENAVSRDHTERMLKTFSYPVQIQDGTIIID
                     RNGECRGTCLNIPGDISSAAFFIVAASITPGSDVLIRNVGINPTRTGIIHILTEMGAD
                     IKVLNQRAYGEEPVADLHIRYSQLKGIDIPASMVPLAIDEFPVIFIAAACAQGKTTLH
                     GAKELRLKESDRIGAMVDGLNQLGVHAEGFDDGILIEGGSIQGGEVNSRGDHRIAMSF
                     AIAGAVASAPVTIKNCANVATSFPSFVTTANMLHFQIEEYS"
     gene            32046..32738
                     /gene="cmk"
                     /locus_tag="U6309_000517"
     CDS             32046..32738
                     /gene="cmk"
                     /locus_tag="U6309_000517"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEN5503972.1"
                     /translation="MVFNNEVPVITLDGPSGTGKGTICHLVAKRLHWNMLDSGAIYRV
                     LAYAARKNSIEPNDIKKLTALARSLNLRFESSVDNETKVILDDENVSQQIRSEQCGQD
                     ASQIAVIPEVRAALLERQRNFAQFPGLVTDGRDMGTVVFPNAVLKVYLYASAEERANR
                     RYLQLQDNGINVSLAQVVEELAKRDARDTARTHAPLKPAEDAVLIDTTGLTIVQVFNI
                     VLKLIDERLNNL"
     gene            32804..34480
                     /gene="rpsA"
                     /locus_tag="U6309_000518"
     CDS             32804..34480
                     /gene="rpsA"
                     /locus_tag="U6309_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEN5503973.1"
                     /translation="MSESFKELFEQSIAGAQFYPGAIITAKVIDIDDDFVTLNAGLKS
                     EGIVAVEEFYDKNGELEVKVGDTVEVALDSVEDGHGETLLSREKAKRQEAWRKLSKCH
                     ENNETVTGLISGKVKGGFTVEIGSIRAFLPGSLVDVRPVRDPSYLEGKELEFKVIKMD
                     LKRNNIVVSRRAVVEEESSADRQALLESLHDGQELHGIVKNLTDYGAFIDLGGIDGLL
                     HITDISWKRVKHPSEVLSVGQDVKVKVLSFDSERNRVSLGMKQLGNDPWVDLVERYPI
                     GKRLQGKVTNITDYGCFVEIEEGVEGLVHMSEMDWTNKNVHPSKVVSLGDVVDVMVLE
                     IDEERRRISLGMKQCVGNPWQQFASTHNKGEKVKGKIRSITDFGIFIGLDGDIDGLVH
                     LSDISWTVPGEEAVKQFKKGQELEAVILAIDPERERISLGLKQLEGDSFASFAETYTK
                     GSIVKGTVTAVEPKTVTVALAEDVSGTIRVSELSDERVDDATTIVKVGDEVEAKITNI
                     DRKNRTISLSVKAKDAQDEADAIKKYSRTEAASTTLGDLLKEKMASKEGE"
     gene            34593..34883
                     /locus_tag="U6309_000519"
     CDS             34593..34883
                     /locus_tag="U6309_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEN5503974.1"
                     /translation="MRILMLVTYLLLIIIGVSFAALNASSVQVNFYFKTVSMPISVLM
                     TIMLGVGVFIGFVLFITRYWRLKIECRKMKNQLKLTEKEIKNLRSIPLQDQH"
     gene            34930..36099
                     /gene="lapB"
                     /locus_tag="U6309_000520"
     CDS             34930..36099
                     /gene="lapB"
                     /locus_tag="U6309_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEN5503975.1"
                     /translation="MINLWPLLLPAAAWSGWWLANRSNSNKDAHVHNRLSREYVVGLN
                     YLLNEQSDKAVDVFIKLLEVDSDTVETHLALGSLFRRRGEVDRAIRIHQNLIARPQLS
                     IQQRKEALMALGQDYMSAGVFDRAERIFLEVVELGGSRDTSSLQGLLAIYQQEKAWEK
                     ALDIIKKLEISTGASFHNQAAHYYCEMASQALKINAMDRAIYCIKQAMNVDPESVRAS
                     LMNATIEIKEGRYKQAIRSLKRVPQQDAEFLTEIIEPLVLCHKELDSMPDCIHYLEQT
                     LEKHPRASVIFVIADYLRQNKGMDIAIDFVADNLSKHPSIRGLNRLIYWHLESAHGKV
                     REKLQMLYDITSKFLDNKPIYRCGHCGFGGKHLHWHCPSCKQWGRMKPIHGLEGD"
     gene            36353..37468
                     /locus_tag="U6309_000521"
     CDS             36353..37468
                     /locus_tag="U6309_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEN5503976.1"
                     /translation="MQFIDLKTQYSLIENDILNSIKRVLNHGQYIMGPEIAELEKELA
                     TFVGVKHAIVNSSGTDALLMALMALDIQPGDEVITSPFSFFATAEVISLCHATPVFVD
                     IDPLTYNLDPEQLERAITKKTKAIMPVDLYGQCADYDVINAIATQYGIPVIEDAAQSF
                     GATYKGKYSCSLATIGCTSFFPSKPLGGYGDSGACFTNDDVLAQKLIEIRIHGQNARY
                     CHHRVGINGRMDTIQAAILLQKLKIFSNEILLRQKVAKRYDQMLSELVKTPYVHECNA
                     SVYAQYTIEVENRDVFAKSMQASGIPTAVHYPIAMHQQQALGYLNYKLGDFPNSEKAS
                     QHVISLPMHPYLQEDEQLKIVAAVRNALLVMNEEAMV"
     gene            37469..38158
                     /gene="pyrF"
                     /locus_tag="U6309_000522"
     CDS             37469..38158
                     /gene="pyrF"
                     /locus_tag="U6309_000522"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEN5503977.1"
                     /translation="MTPKLIVALDFDNQDNALQLVDKLDPNHCALKVGSELFTLLGPQ
                     FVKELVRREFKVFLDLKFHDIPNTVAKACHSAAELGVWMINVHAIGGLKMLQAARESL
                     KIYGKDRPLLIAVTVLTSFEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHE
                     VKIIKQKCGENFITVTPGIRLPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNP
                     HEVVSALLRDL"
BASE COUNT        11917 a         6541 c         8164 g        11793 t
ORIGIN      
        1 aaccgtctat aagtgcttat ataaacttgc aagactaaaa gagcaaactc attctatatt
       61 tcggataatt atctatgatg catttacaag agttggcaaa acatggccag caagaaaagg
      121 aagtgatttt aaacgagcgt ctaccttcgt ttgttagtcc cggatgccat ttgcatgcgt
      181 cttatcatat agagacaaaa gatgattttt atcttattca tcttagagtg caaggtaatt
      241 taaacagtat atgtcagcga tgtatggaaa acttcactgt atgttataat aactccactg
      301 ttattgccgt ttgtcgaagt gatgagagag ccgagcaact tttgagtcaa tatgaatgct
      361 tggtttcttc aaactggcag gttgatttaa atgaattaat aattgatgag ctgcatttgt
      421 atgcaccaca agttcatcct gatattaatg aatgtgctaa tgaaatcaat gaaattttaa
      481 ctgggaaaaa acaaacttct tgatattttg ataattaata cttggattga agttaaaata
      541 tcggtaatat tcccgcttta tgaatgcaat ttgtttagga gtaatacaat ggccgtacaa
      601 caaaataaaa aatcacgctc acgtcgtgat atgaggcgtt ctcatgatgc actaaccaag
      661 cctactctat cagtagatcc aacaactggt gaaacccatc ttcgtcatca catgacccca
      721 gatggatatt acagaggcaa aaaaattatt gacgctgaaa ccgcttacga acaagagtaa
      781 ctttcttgaa gaatatcacc attgcaattg acgcgatggg tggggatcac ggcttggaaa
      841 ttgtgattcc tgcctgtatt cgtgctgtta aaaacaaccc tgatttgaaa ctattgcttg
      901 taggagtcca ggacaagatc agtgcttctt tgaaaaaaca tggcatgttg tcctgtcagc
      961 aatttactat cgtccacgca tcagaagtgg ttacaatgga tgaattgcca tctcatgcat
     1021 tacgaaataa aaaagattcc tcaatgcgaa tagcaattaa cctggttaag gaaggcagag
     1081 cgcaggcttg cgtcagtgcg ggcaatacag gtgccttaat ggctaccgcc cgatatgtct
     1141 taaagacttt accggggatt gatcgcccag caatagtatc cgagttacct accatgggtg
     1201 gaaaaacaag ggtcattgat ttgggagcca atgttgattc ttgcgcagag catttgtttc
     1261 agtttgctgt gatggggtct gcattgatac aggcaataga aaataaaccc aaacctaaaa
     1321 ttggtttact taatattggc gttgaagaaa tcaagggtaa cgatcaggtt aagcgtactg
     1381 cacatatgct ggctgagtgt tctgttatga attatgtggg atacgttgag ggcgatcact
     1441 tttacagtgg tgatgttgat ttagtggtct gtgatggatt tgttggaaat gtggcattaa
     1501 aagcaagtga aggtttggca aagcttttat taactgtcct taaagaatca tttagtagaa
     1561 attggttaac aaaaattgct ggtttgattg ctttaccagc cttaaaacat ttaaaaaatc
     1621 gtttggatcc atcgcgatac aatggtgcta gtctactcgg gttgaatggt atcgttgtca
     1681 aaagtcatgg tggagccaat gaagtgggtt ttcaacatgc tattgaacag gctgttcttg
     1741 aagtaaaaaa taatgttgtc gatttagtgc gtgatcaaat aaatgatttt ataaatcaag
     1801 gattgttgtt atgaaaaatg ctgttattaa tggcactgga agttactctc cagagagaca
     1861 aatgactaat gctgaactgg aaaccatgct tgatactagc gatgaatgga tagttaccag
     1921 gactggtatt agtagtcgta gtgttgctca agaacatgaa acaacatctt atatggcctc
     1981 cagagcagca gagcaagcac tagaggcatc aggccttgat gctgaagaaa ttgatttgat
     2041 attagtagca acatgtaccc cagattattt ttttcctagc gttgcctgtc acgtacaaca
     2101 tgctttagga atcaaaagac ctattccggc ttttgacatt ggagctgcat gcagcggttt
     2161 tgtttatgcg atggatgtag cgaaacaata cattgctaca ggggctgcca aacatgttct
     2221 tgtcgtaggc agcgagagca tgtcaagagc ggtagattgg actgatcgtt ctatttgtgt
     2281 cttattcgga gatggcgcag gcgctgttgt tttaagcgca agtgatcgcc aagggattat
     2341 gggtagtgtt ttacattctg cctatgactc tgataaatta ctagtccttc gtaattcaac
     2401 ttttgaacaa gatcgtgcaa cgattggaat gcgaggtaat gaggtattta aaattgctgt
     2461 taatattatg ggtaatattg ttgatgaagt gttagaagca agtcatttaa aaaaatctga
     2521 tattgattgg ctgatacctc atcaagccaa tatacgcatc atacaagcca tagctaaaaa
     2581 attatctctt cctatgtcac atgttattgt tacaattggt aaccaaggta acacatcggc
     2641 tgcttctatt cctttagcac ttgattattc tattaaaaat aatcgaatta aaagggatga
     2701 aatattatta attgaatcct ttggtggtgg aatgacctgg ggcgctatgg ttattcgtta
     2761 ctaaattaat taataaggat taattctatt atggtaaaat cagcatttgt atttcctgga
     2821 cagggatcgc agtctatagg catgttgtct gactttatgc cacagtatac tgttgtcacc
     2881 gatgcttttg cagaggcttc agatgcctta ggctatgatt tgtgggattt ggttcaaaat
     2941 gggccagaga caaagctaaa ccagactgaa tacacgcagg ctgccatgtt aactgctgat
     3001 gttgcaattt atcgtttatt aatgcaatta ggcgtacctc agcctcaagt aatggctggc
     3061 catagcttag gggaatacgc tgctttggtt tgcgctaatt ctttatcttt aaaggatgcc
     3121 gcacagttag ttgctcgtag aggacaggtg atgcaaaacg cgatccctct gggagagggg
     3181 gctatggctg ccatagtagg cttatctgat gaacaggttc gttcattgtg tgagcaagcc
     3241 agcacagcca atcatcttgt taccccggcg aattataatg ccctgggcca aattgttgtt
     3301 gctggacatc gagtggccgt tgataaacta atccaattgg ctgaagaggc tggcgctaga
     3361 ctggcaatga taattcctgt aagtgtgcct tgtcattgcc ctttattaaa aaatgctgcg
     3421 gaattatttg ctgaaaatct tgctcaaatt gaatttcaag ttccttcagt cgatgtaata
     3481 agcaatgttg atttaagtat ttatcattcc acccaacata taagagataa actcaaagaa
     3541 caactataca gccctgtgcg ctgggttgaa acagttcagt taattcaaca gcgtggaatt
     3601 gaactggttg tggagtgtgg gccaggaaaa gttctgaacg gtttgataaa aagaattgag
     3661 agaaatttaa ctacgattag cgtttatgac accattagtc ttgatcaagt catagaacgt
     3721 ttacatatcc cagtttgaga ggaaactaga tgatcaattt agaaggtaaa attgcattag
     3781 ttactggtgc cagccgtggc ataggtcgtg ctgttgccga gaaattggca agaaatggtg
     3841 cctttgtttt tggaacagca accactgaac agggtgctgc gtctattaat tctttttttg
     3901 agaaagaaaa attggctggt aaaggaatcg ttttggatgt aaccagcact gagcaagtag
     3961 aaaatttgat gtctaccttg tctgataatg atcagtgtcc ttctattctt gttaataatg
     4021 ctggcatcac cgcagataat ctgttattgc gtatggatga tgaggaatgg tacaaagtaa
     4081 tagaaacaaa tttgaattct atttacagaa tgactaaagc ttgcttaaaa cctatgttca
     4141 gagcacgttg gggaagaata atttctatag gctctgtggt aggtgccagt ggcaattctg
     4201 gacaggtaaa ctatactgca gccaaggctg gagttattgg tttttccaag tctttggcac
     4261 aggaaattgg tagtagagga ataactgtaa acgtggtagc ccccgggttt atcgacacag
     4321 atatgactac tgcattgcct gatatggtga aagaggaaat gttaaaaaga attcccatgc
     4381 gtaagctggg ccagccagaa gatgttgctg atgctgtagc ttttctagca tcagacagcg
     4441 caaaatatat cacaggtgaa acaattcatg tcaatggcgg tatgtatatg agttaaatgc
     4501 acttgcgtta tctgtataat tgaataaact agccgtcagt atttaataat ttctatcaac
     4561 cgaaagagga aaattacgtt atgagtacag ttgaagagcg tgttcgcaaa attgttgttg
     4621 aacaactggg tgttaaagaa gaagaattaa agaatgatgc atcctttgtt gatgatttgg
     4681 gtgctgattc cctggataca gtagaattgg ttatggctct tgaagaagaa tttgaaacag
     4741 aaattcctga tgaaaaagct gagaaaatta ctacaattca agaagcaatt gattatattg
     4801 aatctaactt aaataaagaa gaagcttaat ttttttaagg agctctcatt gaatagtcgg
     4861 cgtgtggtta ttaccggcat gggtatgctt actcctgtcg gacttaatgt agaagaaact
     4921 tggcgaagta ttttagctgg ggttagtggg gtaggtttgg tagaagattt tgacactact
     4981 gaatacccaa ctaagatttg ggctaaggtc aagaatttta atgttgagaa tcacatgcca
     5041 attaaagacg cccgcaaaat ggacgtcttt acacaatacg gtatggctgc tgccgatgaa
     5101 gcgatgcttg actcgggttt aaaaattgat gaaatcttat ctcgtcgaat cggtgtcgca
     5161 gtgggggcag gtattggtgg tattcaaaca atcacaaata accaggataa actggttgcc
     5221 ggtggaccac gtaaggtttc gccatttttt attccggcag gtattatcaa tatggttgct
     5281 ggacagattt ctattaaaca taatttaaaa ggacctaata tttcggttgt gactgcatgt
     5341 actaccggaa cacataatat cgggctagca gggcgcataa ttgcttatgg cgatgccgat
     5401 gtcatgattt gtggtggagc agaaatgacc acaacgccac tttgtcttgc tgggttttca
     5461 gcagtaagat ctttgtccaa gcggaatgat gagcctgaga aagcctcaag gccttgggat
     5521 aaagacagag acggttttgt tatgggtgag ggtgctggta ttttggtttt agaagaatat
     5581 gaacacgcta aagctcgtgg agccaaaatt tatgcggaat tggttggttt tggtatgtca
     5641 ggagatgctt atcatattac tgcacccgat gaagatgccg atggagcttc acgagctatg
     5701 gaggcagcca tccaggatgc cggtatcgat cccagacaag ttgattatat taatgctcac
     5761 gggacgtcga cttatttaaa cgatttaaat gaaacaaaag ccattaagcg tgttttccag
     5821 aatcatgctt atgatctggc ggttagctca accaaatcca tgacaggaca tttgctcgga
     5881 gctgcaggtg cagtagaggc aattataagt attttagcca taagagatca ggtggctcca
     5941 cctacaataa atttggataa tcctgatgaa ggttgtgatt tgaattatgt agcacatgta
     6001 ccacaaagca gaacaattaa ttatgttcta agtaattcac ttggttttgg tgggacaaac
     6061 ggtagcttac tgttcaagcg aatctaaatg caattctcta gacataaaaa actggttatt
     6121 cttgttttat ccttgttttt tatgtctttc ttgtttttat tttggaatat atatcactta
     6181 ttatatagac caataattcc gaaaaatgct tctccaatga ttattgcctt ggataaggcg
     6241 acgtccgctt accaatttgc ttatattttg caagataaaa aattaataca ttctgccaaa
     6301 ttttttctat tgattattcg ttttgaagga ttatcgcatc agttaaaggc aggcgtgtat
     6361 caaattactc ctggcgaaac tgcaatgaaa ttactacatc gagtagttgc tggtgatgta
     6421 attacgcaaa actttacgat aatcgaagga tctacacagc agaaagtaga ttatgaccta
     6481 agacaagcta attatttgaa atacaatcca gaagattggg caatagtaaa agaaaattac
     6541 ccaagtgcag agggattact tctagcagat acttatcaat accaaggtgg gagtaatagt
     6601 agggcattat tagagcaagc ccatcgtaat ttattaaatt atcttaatac cagttggaca
     6661 aacagagcac caaatttgcc ttataaaact gcttatgagc tattgattgc tgcgtcaatt
     6721 attgaaaaag aaactgcaat tgcgcaagag aaaaaattga tttctggtgt gatggtaaac
     6781 aggttaaaga aaaaaatgcc attacaaatg gaccctacgg ttatctatgg actgggaaat
     6841 caatataaag gaaaattaac acataatgat ttgctaattc aatcaccata taattcttat
     6901 ttgaacagag ggttgcctcc caccccaata gcaatggttg gtaaagaggc catagatgct
     6961 gctgcacatc cgcaattgtc caattattta tactttgttg ccaaaggaga tggcactcat
     7021 caattctccg aaacttatga gcaacaaaga caagcaatta accaatacag acgcaaggat
     7081 tattaatgtc atcgttaacc gggaaattga tagtgattga aggtttggaa ggggcaggaa
     7141 aatctacagc tgtcaattta gttgttgagc tgttatccca aaaaaaaatc agtactataa
     7201 caacgagaga gcccggagga acaagaatag gtgaaatttt aagaagtatt atcaaaaatc
     7261 cagagtacaa taatgttctg gatgacagaa gcgaattact tctcctgtat gccgcaagaa
     7321 tacaactgat tgagcaagtg atcaaacccg ctttaaatga aggccagtgg gtcatagctg
     7381 atcgatttga gctttccact ttagcttatc agggcggggg tcgcaaaatg gatatgcggg
     7441 ttattaatga gctatcgaat ttctgtttaa aaggattcaa acctgatctc actttatatt
     7501 tagacattaa tcccgagctt ggcatgataa gagctaaatc aaggggaaaa tttgacagga
     7561 ttgagcagga gtcaattgag ttttttcata gaatacatga tacatatcat attcttgtta
     7621 aacaaaaccc ggaaattatg atgattgatg ctaacaggtc tttggaagag gttcaatctt
     7681 ctattcaatc agtaatcgag gagtttatag agcataacat atgattactt atcaagaaca
     7741 atggcagaaa atgcaaatgg ccttggcggt aagacgaatt cctcaagcca tgctattttt
     7801 gggttctttg cattgtggtc ttatagaatt tacaaatcag gtgatcaaat tactcttgtg
     7861 taaaataaaa cagaatgagc cttgtttgac atgcatggat tgccaaatga tagagcgttt
     7921 tgagcatccg gatgtggaat ggattaagcc tgaaagaagc ggtggtatta ttaaaattga
     7981 tcaaattagg ggattgcaga gcacggcgtt tctaacacca cagagaacta atcataaact
     8041 tataattatc gaatcagccg aatcaatgaa tattgcggca tcaaatgcct tattgaaaat
     8101 tctggaagaa ccagcgcaac atactatctt tattttgtta gcgcaacaaa ttggcaatat
     8161 accacccacc gtattgagcc gatgccaaat gacgcaattt gctgtttttg atgagttgcc
     8221 ttgcaacaat ttattggctt taggagagta ctatactcag gaatcgaata gcacaatggt
     8281 tttcaagcat tcagaatcca ttttagaagg cttgattgca attattgagc gaagagagca
     8341 tccatgcact ttggcttcac aatggattca acatgaatta agtgcttttt tgtggttttt
     8401 atatttgctc tattctcaac tacagtatat gcatttgttt cgagtggctt attctggtac
     8461 gttaacaaaa caattgaata agctatcctc attactcaat ccggttatga tttttgccca
     8521 agtagataaa atcaatactt tgttaagaaa attaagccat aatatgaata tgaatcatac
     8581 attggtcttg gaagatttat tattttcttt ggttgttgat tattaattga gtttaaagga
     8641 attaaacgat tgcgtgtgaa ccgagttttt tattaagcaa ctgaagtaag caagatgatt
     8701 agttaaaaca ggactattca aggaggatgt ctattgattg agcggaggag tcatgaccga
     8761 aaaaccacag cttataaact gctcatttcc caatgaagca tctttgtaca tggcttacat
     8821 gccttttgtg aaaggtggcg gtctttttat aagaactcaa cacgtttatt ctctagggca
     8881 gtcgcttgaa ttatctgtta aattgctcaa tgaaaatgaa ccatatttag tcactggtaa
     8941 aatagtctgg attactccta aaggtgcaca aggtaataag ccaccgggag ttggtattca
     9001 atttaccggt gaaaacagtc gttatctatg taacaagata gaaacttatt tggcaggaat
     9061 gctaaaatca tgtcagatta cagatactat atagagtttt catttattca tgaacaatat
     9121 attcattgat attattttgg ggtataaagg tgctgatcga ctctcattgt catttaaatt
     9181 ttatcgattt gactcatttt aatcatgatc ttgctcaggt aatcaatcag gctcgcgaga
     9241 atggagtgga gcatttcttg tccgtttgtg ttgagttgag cgattatcct caattagagc
     9301 gtttagctaa ggattatcct gatattagca tttctgttgg agtgcatccg aatagtgaaa
     9361 tgaattatcc tgttacggca cagatattat gtgaattggc tcaaaatcct gcctgtattg
     9421 ccataggaga aacagggctt gattattatc gtaatcccac tgaggaagct caatctttgc
     9481 aacgagatcg cttcagggaa catatcaagg cggcacttat aactgccaag ccgttaatta
     9541 ttcataccag gcaagctgct gaagatactt tgttgatgat ggctaaggaa ggggctagtc
     9601 agataggcgg tgtcatgcac tgttttgctg aagacttgga tattgcgcaa agagcaattg
     9661 atttaaattt ttatatatca ttttccggga tagttacttt taaaaatgct gtttcattgc
     9721 aagatgtagc caggcagata cctttagaga agatattgat agagacagat tcaccttatt
     9781 tggctccggt tccacacaga ggaaagcaaa atcatcccgc tttggtaaaa cacgtagcag
     9841 aaactatcgc acaactcaga gggatggatt tccaagagat agcagaaatc actacaaaca
     9901 atttttataa ttgttttaat atcaaacgac aaggataaat cattggtaaa tcgtagttct
     9961 caatttcaaa gcagaagcag taattttttt gcctgtgaat aaaggtttta gtagtattga
    10021 tatatcctat tgcaaattta gagtaattaa tgagtttata tatcggtttg atgtcaggta
    10081 ccagtatgga tggcattgat gctgccttgc tggagcttcc ttccaatcaa ttgattcacg
    10141 ggattactaa acaatacagt gatgatgtca ggagaaatct tgatgattta attatgggta
    10201 atcatttaac cctggcttcg atttgccaac tgaacacgct aattggcagg gagtttgcag
    10261 aagcagttca ccaactcttg attgaaataa aagtgcatcc aaaagaaatt caggccattg
    10321 gtagtcatgg tcaaactgtc tgtcatgata caagtggaaa tatcccgtat actttgcagc
    10381 ttggctgtgg ccatacgatt tcttctttaa ctggcattac cgttgtagca gattttagaa
    10441 caagagattt ggtgaatggt ggtcaaggtg cgccttttgc tcccctttac catcaacaga
    10501 tattttctaa agtgaatgag tctgttgctg ttgttaacat cggtggtatt gctaatgtta
    10561 cctttattgc aaagaatcaa atgactagag gttgggatat agggcctggc aattgtctga
    10621 tggatgcctg gatttataaa aataaaggag tcctttttga taaaagtggg gtttgggcga
    10681 gccaaggtga agtgattcat cctctacttg aatatttgtt acaagatcca tttttccatt
    10741 tggattcacc taaaagcata ggcaaagaat atttttcttt atcctggcta caaaaacatt
    10801 taaaaccgga ttacacacca gctgatattc aggctacttt gttggcactt acagcccata
    10861 cgatagctga aacaatattg aatgaatcag gagaaattaa acaattatat ctctgtggag
    10921 gcggggccca taatacgcat ttgaaagaga atttagctcg tctactgcct gggataacag
    10981 taaaaagcat agcagagtta gggattagtc ctgattatct tgaggcaatg atgtttgcat
    11041 ggttagcagc acagacgatt aatcaaatac cagttaattt aacatcaata actggagcta
    11101 aaggtattgc aattttaggt gcagtgtatc ctataataaa atcatattga caaactaaag
    11161 aatcataagg tttaatttca taaaaattaa cagagtgatt ttaaattgac cgcacattac
    11221 aacgtcgctt caaacagtgg agcaagaaat acacttgcaa cgtcttattt tatttttttt
    11281 ctagccgctt cattttactt gtatgaattc atcctacaag tagcaccaag cgttatggca
    11341 gagtcgatga tgaaaacttt tggggtgacg ggtcaaggct ttggtgtcat ttcagctttt
    11401 tatttttatg cttatgcccc tacacaattg ccagcaggag tattatacga tagatatgga
    11461 ccacgcaagt taatgacttt tgcaatcgtt ttgtgtgcat ttggttctgc cttttttgct
    11521 tccactgaca gtgtatttac agcttgcata ggccgatttt tgatcggaat aggctctgcg
    11581 ttttctttta tcggtgtttt ggtccttatt tcacgctggt ttccccctta ttattttgca
    11641 attctagcag gggtagcgca gttgatgagt tcagtaggtg ctatgtttgg cgaaatgccg
    11701 ttggcagctt taattgatct ggttggatgg agaagtgcga gttttatttt agccggtgtt
    11761 ggatttctct tggctatttt gttttgggta tttattcgag attatcctca tcagcagaat
    11821 cagacaatcc ctgatcatta tttaagagat gaatggaagc gcttagttgc tgtctgtaaa
    11881 catgcttata catggattat cgggggttat gcttttgcaa tttggactcc tattgcagta
    11941 tttgctgcac tttggggagt tcctttttta caggaaaaat ttcaagtaag cgtagtggct
    12001 gcctcaggct tatgcagtat gatttggcta ggcattggtg ttggtagtcc cttgttaggt
    12061 tggttgagtg ataaattgga gagtcgtcgt attgcattga ttatcagtgc ggttttaggg
    12121 ttgattgcaa ctctgattat cctttattgg tctgagttat cttataattg ggcttatgtt
    12181 gtcttgttcg ttttaggatt aggtgctgga ggacagacgg taagttttgc agtagttaaa
    12241 gaaaataata ccccggaatt agttggcaca gcatctggtt ttaataattt gtctgtatta
    12301 attggtgggg ccattttcca gccactcgtt ggttatatct tgcaacagac aggatcatgg
    12361 cgaattgtca atggtgctca tgtatacagt atatcaagtt atcagactgc tttgctcgtt
    12421 atgccgatat gcttcctggc cagtttgctt attgccactt ttttgcttaa ggaatcacat
    12481 cctgcgcgca gagattaata acagatttat caattcaata ctctagcccc gatatctttt
    12541 tgcttaggct taaataatat aacgtgagtt atgggtaaag attatatagg agccagtcga
    12601 tatccggaat ccattcaggc tgaggagatg cgtaagcatc gtctcgaagc cttggcacga
    12661 gacattaaat tccatacaag gcttcgtgac ggcataaatg cctcctcagc ctgaacggct
    12721 tgagataacg gctgatctta ctcataactt acgttaatat aattggagtc ttggtagtgt
    12781 gcggataaag cgagctaaga aaatattcag ctaaaatttg ctgtaatctg ttggtaattg
    12841 agagacctta attcttatat tagacttttt gaacaagctg cctctttagt ctaggtgtcg
    12901 cattgcaaaa gttttaatat agcaacaatt tggcatgtta actccattca cctgtttatg
    12961 tttgcgttat tgacctaaaa caaaggaatc aggttatgta aatgtggcct gttcttatga
    13021 gttagccaca ttactatttg cttcatcatg aatttcagat ttcttgggag tccctacaaa
    13081 aaaagttaaa ccgataaaga ttaaaaataa taatccaacg gcattacaat aaagggcgaa
    13141 atgcagatta ttgtgatgtt gataaaacag gttagctatt tctattccta tagccccaat
    13201 gatcataata cttaggctga ttaatgccga ggctgttcct ttgcttacag cggttacaaa
    13261 taaacaatac ctgttcaagg gggcattaat tacactcagt gagaaaaagt aaattatgat
    13321 tcccgggatc aaatacacat aattattgcc ataaaaataa gggagaagag ctgtcagagc
    13381 aagtcccacg accattatga tacaacctat aaaaataatt ctctcgattt tatatttgta
    13441 agttaaatga tgtaagcacc agtttcctaa aattgttgcc ccgaagatgg gtagttgcca
    13501 aaggccatat tgaattacag ttaattttgc ttcagcaatt aatatgattg gcgccagcgc
    13561 aatccaggcg atgcatggga tccctaccag accttcagca attgcactat agcaaaaggc
    13621 ctgattagat aataacgttt tataatttct ggttatagcg ttcagtgcga acggtgtttt
    13681 aggtataagc tggccgtctc tttttacctg gcctatgggt tctggcataa accgccataa
    13741 tccccaataa gctaaaagag cacccaaagc aataataata aaaatcaaac gccaggacgc
    13801 ataatgaatg attatagctc ccaatagtgg ccctaataat ggggctaata ttgcggcatt
    13861 agccatgata gcaattaatc ggatagcatc catttcttca aatatttctt gaattgtggc
    13921 atagcctata acgccaatga aacacaatcc cattccctga aaaaaccggg cgattaaaaa
    13981 ctgattcatg gagtgtgagc ttgcaatcaa aagagtgaac agaaaaaata agcatgatcc
    14041 tattaacatc ataggtcttc tgccataagc atctgatagt gggccaagta tgagctgcaa
    14101 actggcccct ccgaggacat agacagttag agaggtggca acaacacttt catgcgcgtt
    14161 gaatgattta acaacattga tcatacctgg catgatcatg tcattggcta tataagtcaa
    14221 aaactcgtat aaaaccagga agcaagcaaa gataatggcc tgtctgtatg aaattttaat
    14281 tagtggctca gacatagcat taaacctgaa tcaatcgttt ctaaaaggcg gtctatcagt
    14341 atatacattt atttcataat ttggtagaaa atctgataaa tgttttggta agagacataa
    14401 aatctgccat atactgagaa taaatcttta caatagcaac tgccacttaa agtttgttta
    14461 cggcaagtcc accatttgat aagatttact tacttctaaa atctggttct tatgatctga
    14521 tcagtatgcc caaagaaaaa gtcgttatta ttatccctac ctacaatgaa gaagataata
    14581 tttctaatac gattgatttg attcttaagg agacttactc actcgcagat tatcaggttg
    14641 acattcttgt atttgatagc cactccacag attctacagc ccatatagtt cgagagctaa
    14701 cttatacctc tcaacgtgtt cattttgcac aagaaccaca gaaaactgga cttggttccg
    14761 cttacctgca agctatgaga attgcaataa atgagttaaa tgcagatgtt gtatttgagt
    14821 ttgatgcaga tggttctcat caaccttgtt atattgctcc catgttagag ctacttaaac
    14881 agcatgatgt agttgtgggg agccgatata tttcaggggg aaggattccc tcaaactggg
    14941 cattccatcg taaattatta tctgtgggag gtaattgggt tgctcgtatg ttattaactg
    15001 gaaaatataa agatcttact tctggtttaa gggcaatacg tactaatctg ctaaagaaac
    15061 tattgccttc aaacttccta tccaataatt acgcgtataa acttgagtta tactggcttt
    15121 tacacaaggc aaaagccagg atcgttgaat ttcctattca atttattgat agagaaaaag
    15181 ggaaaagtaa attacctaaa aacagtatga ttgacacctt acgtgttctt tttacccttc
    15241 gcttcagaga attacgccag tacctaacta tgtgctgtgt tggctccatc ggtgcattga
    15301 ttcaattcag cttatacaat tggttgcgca gttcgaacta ttccgctctg gtatccagcc
    15361 aagttgctat cactgctgct attatatcca attttatttt tcaccattcc attacattca
    15421 aacaaaaaac tgacttccgg ataactaaaa caacgtttag ccgttttaca caatttatta
    15481 tttattccac atttatggtt tattttcaaa gcctatgggt tcatattttt gtccattcct
    15541 ttggacaagg tgttttgaaa gaaaatatca ttgtcgcctc aggcttgggc attggatcta
    15601 ttatcaatta cctgttttat tcaaatatta tttggtcatc aaaaggcgct cattctctca
    15661 attcaaactc ttctaaagaa actcaaattt gaaagcataa gtgtaatgag attagataaa
    15721 ctaaaccctc aataaaagca tttttttaca aagagattgg gggtataact cctgtttcat
    15781 ttaagtatcc agcttattac tgcaccaata ggcgtaaatg attggcttac cttttccatc
    15841 aaaagtagct ggcaaattta atgattgaca ctgggcaaag tattttttaa cacatgataa
    15901 tctatcaaat gtatcgatat atagcacttc gccaagggta ttcagattag gtgaccaata
    15961 cccgtattga ttttgcattg aaccgcagtc gaaggtaaaa attgttggtt tatttttaag
    16021 aaaaaacagc atgcgcgcct caaaccatcc tggggttatg actatgcttg ggagtttatc
    16081 attagactga ttaatttgtt gtatcgattg ataataagcc agtttttttg aatacccaaa
    16141 actgaaaaga gtggtattgt ccatcaaaat gattccactg gtaagagtgt agaatacaat
    16201 taataaataa gtagaattac gataagccca ctcttgatag caataaccag ctaataagct
    16261 ggcagtaatt atgtattggc tcaaccaata tcctcttatc gtggctttgc cggcgacaaa
    16321 taaatagaaa agaccaaatg ttacgcaaat aatgaggcac aaataaaata caggggattt
    16381 tctaggcgtc cctttggata gatataacgc tggtggaatg aataaaaagt taattgcagg
    16441 gaggaaaata ctaaaaaagg catgcaagac actccccatc gcatgatgag tattttgcgt
    16501 ttgatgagta cttaactggt agcgaatgga tacccaatca tgttgagcat tccataggat
    16561 aaccgggcta aagataatga ttgccagggt taatgccaaa taaaaatgcc tggatttaaa
    16621 aagtcgtcta tagggtgttg caaccaaaaa aataagcaag gccagcacga gaacaatacc
    16681 tgagtatttg gataaaagca taaggcctat actcaagcct atgtaataca aatcctggtt
    16741 tcgattaaac tggatatacc ttattgtaaa gtaaagagtt aatgcccaaa aaagggttaa
    16801 aggtgtgtca taggtagttt gattgatcaa atctaaagtg actaatggtg agaataacca
    16861 aatcaaaacc gtaacataac tggcttcctt attaaggcat aatcttgctg ttttataaat
    16921 tataaaactg gtcaaggcag tagaaactat agtaaccagg tttaaagcaa agagagtatc
    16981 tccaaataaa agtgtggcta tgcggataaa gtaggcgatc atgggagaac catcatagta
    17041 tgatagagcc aaatgcctgc tccaatccca gtaataataa ctatctaatc caaggaggtg
    17101 aattggtgca attaatagaa tgagtagaaa atagaggcta atgtagcgat tcatgtgcaa
    17161 aaacattcag tattcccagg aaagtaatga tgaattgtat gttattttat tatcagtttt
    17221 tatataatgc attcagtttt atcattaatt aataaatgac gtttatgaaa ttatattttt
    17281 ttggtgatga taataataaa cccaaattaa gcgaacattt aatcatttac atactcagtt
    17341 tgctagatcc caagtctcag tctgttgctt ctttagtgaa taaagactgg tctcggctgg
    17401 ttcaattgac acggcaatac ctctctatgt taccggtgat tcatcgaata cctttatttg
    17461 cgaatttggg tcttgacgtt ttgaaattaa aattaatgac aggtggtatg acgaattcca
    17521 catttaggct aagaatcaaa aatgcgcctg agaaatgggt tatgcggatt cctggaatag
    17581 gctcttcagc atttataacc agaagagatg aagcccacaa tgctaaacaa gccgaaaaac
    17641 ttggactgaa tgttccaata gctttctttg atccagaaga tgggctgcag gtaacgcgct
    17701 ttatagaagg agtacattct ctggatgata aagctttggc ccgcaaggaa attctgcatg
    17761 aggtagcatc tatgatgaga agacttcata aatctcctcc ctttgataat aacacgcttt
    17821 tttttgagcg aaatgaagag ttattggatt tattaaaacg aaaacccttt aatttcccag
    17881 gggaaataga atttattgag caacaaatga aaaatctaaa ggagattttt tcttcttatc
    17941 gtattcagca atttccttgc cacaatgata caacgccact taattttatc ttgtccgaaa
    18001 atccagaaat caagggttct gaaaagattc atcagattga ttgggaatat tcaagtaata
    18061 atgattttat ttgggatttg gtttatttta tggttgaggc caagctaaac cgtgaacaac
    18121 agcttattct gttgaaagct tactttaata cagaagagct gagtggctct ctgttggcat
    18181 ggatagaggt ttataaaccc attattgagt ggtggataac catttggtca tggacacaat
    18241 tggcaaatgg agctaatgca gttgatttga gttcttatga gcaactaggg cttgaaaggt
    18301 atcataacac cttaagtcac ttgaaaagtg agggctatgt caaagccttg gcactgattg
    18361 aaacagatag tcatagcctt acttttacgg ggcagagacc tttttagcgg tcgccattat
    18421 ctagacagga caactcgttt acagtgacga ttaagatctt cagagcattt ttttgagaaa
    18481 ttcctgtaaa tgcatcttgt ctttctcagc acctaaatag tttttgagtt gtgctatgtt
    18541 tctgtcataa ccttcctggc taattgtgcc acgcatcagc tcaaatctta aatatacaca
    18601 ataggtgttg agtacgtctg tttcacaata atctcttatt tctttcaatt ggcctgcctg
    18661 gtattgttcc caaactttag agccactcat tcccattttt ccagggaaac ctaacatggt
    18721 tgaaatgtca tcaagagggg caaatgctct gttctgatat cctgcaagaa catccattag
    18781 atcaatatgt cggtagtgaa aacggcttag ataattgttc catttaaagg tttgttgatt
    18841 ttcccctgtc tcccaataag tgggcgcact aatactatgg tatagggatc ggtaatgcag
    18901 tacaggaaga tcaaaaccgc tcccattcca gctcaccagg gtgggtgtat gtttgtcaat
    18961 tccagcaaaa aaacgagtta ttaattcttt ttcatcagaa ttttcatcgc caagagacca
    19021 tacttttatt tgatttccat gactgattac cactgatata gcgcagattt tttgtaagta
    19081 atgaggcaaa aaatcattgc ctgctttctc acgtcttaag gcaaacatag cgcatgctgt
    19141 atcttcatct gataagccat caagtgcaaa aagctttcgc cctgactcaa tatctgggat
    19201 tgtttcaata tcaaaaacaa gtattgtcat aatttgttgg tccagttcgg tcttaattct
    19261 tatttttgca aatttgcttg aaactatcgc caagtttcgg atttgtcaaa atcacaattg
    19321 ggatatctga ttgattctat catgtgaagg aatagaagta actttaaatg gcatagattg
    19381 tatgcagtat atttgataca atatttttta aattgatacc ttatatcgta gcattgatat
    19441 tcaatactgc aggatgagtt ttttatcatt aaatcatgaa gattcgattt actttacttt
    19501 gttttagcat ttttcttacg ggctgcgtca gtaaacctcc agcagacatt aataatattt
    19561 gtaatatatt taaacaatat ccgaaatggt atgtggattc aaaggatgtt gagcgacgct
    19621 ggaaggttcc ggttccggtc caaatggcaa ttattcatca agaatcaaaa tttaatgctc
    19681 gagctaggcc accaagaacc aaactgttat ggataattcc atggacaagg ccatcaacag
    19741 cttacggata tactcaagcg ttgcgttcga cttgggacaa ttataaacgc accaatggat
    19801 ggttttggtc atcacgtgac gattttggag atggtgttga ttttatagga tggtatgcta
    19861 accaggcgaa tattaaggca ggtattcccc gaacagatac ttactcactt tatttggctt
    19921 atcatgaagg gataggcggt tatcaaagga agacctattt gaaaaaacca tggttaattt
    19981 ctgtagcgcg caaagtgaag gctaaatcac aactttatgc aatgcagctc aattcttgtc
    20041 aaggttcgtt gaagtcacgt tcctggtggt aattaagatg gcctaattat gttcttgcca
    20101 gtggaaataa agtcagattt ggaacatata tgattttgtg tgtttataat aatcgccata
    20161 acacgaagtc aatcttaagt aaggtataca tttctatttt taaatagaaa atcgggtaaa
    20221 atactgttta tttaacagaa taaagcttga aaagaaaagg tgagtgatgg tctgcttatg
    20281 atgtgcctac tcaatcctta tagtcttaaa aaacctgttt tttgagctgc tcatttagtt
    20341 ttttaagact atgcatgacc aaaattattt gtcaatgtgc actaaataat tataaaattt
    20401 tatagtgcta ctatgttact atattcgaaa gtttataatg attacgaatt atttaaggag
    20461 ttttggatgc gattaatgct tttaggtgga ccgggcgccg gcaaaggaac ccaagcttca
    20521 cttttaatta accgttataa aattcctcaa atttctaccg gtgacatgct gcgtgctgcc
    20581 atagcaaaag gtacgccctt aggattaagt gcccaaaaaa tcatggaatc cggtgggttg
    20641 gtgtccgatg atattattat cgggttagtg aaggaaagac ttaaaaaccc tgattgtgat
    20701 agaggattcc tgtttgatgg ttttccaagg acattggtcc aggccgaagc tttaaaagat
    20761 gcagaaatcc atttggatca tgtgatagaa atagccgtcg atgacgaaga aataattgaa
    20821 cgcatcagtg ggcgacgcat tcaccagcct tctggtaggg tttatcacgt tgtgaatcaa
    20881 ccgccgaaaa atccgggtgt agacgacatc acaggtgagc ctctgattca acgtgatgat
    20941 gataaagaag aaactatacg taagcgctta caggtttacc atagccaaac agcgccattg
    21001 gtgcaatatt ataaagaatg ggctgaatct ggctcgaaag aagctccaaa atttcacact
    21061 attagtggta caggtacagt agagcaaata tttgacaaca tcgtaacgat actcgaaact
    21121 taggagttaa catgccgacc cagactatta acagcgctga atttgaatca ttaattaatg
    21181 ataatgagat agtttttatt gatttctggg ctgagtggtg tgctccctgt aagcaatttt
    21241 caaaagtata tgagcaagtt tccgatcaat ttccagctat taaatttgtt aaagttaaca
    21301 ttgaagagca acaagagtta gcagattttt tcgagatccg atccatccct catcttatgg
    21361 ttttcaaaga cagtattgtt atttattctg atgctggaag catacccgag tccactttaa
    21421 aagaattggc gcaacaagca cttgatgctg atgttagtga aatacgagct cagcttgagc
    21481 agaaggataa gtagatatac tagacagata atccgcaagt tcttttttac tgacattatc
    21541 catagtaagg ccgagcttag tgcggcggtt aagtatgtca tcacaataat tcgcccactc
    21601 ttctaaaata agataatcta cttccacctg gtagagtgat gggccatatt ttttccccat
    21661 tgcttctata ttgttgcatt gcgaaagaaa aatttcagta taacaaccat aggtattgag
    21721 gtaacgattt aatagttcat catccagcca atgatatttt gttttggcgt aattgacata
    21781 ttgagtaaaa ctcatatccg ctaaagttgc ccccggtaag ggcgtgtgtt gtgtttttga
    21841 tggtttcatt tgtggaaata caggggtaag ttgatctaca acttcttcag ccaactgcct
    21901 gtaagtggtt atttttcctc caaaaattgt aataatggga gcagggtagt ggttaaactc
    21961 ataggaatag tcacgactta atgatttgac ttccttgcta tcatcagcta ataaaggcct
    22021 aataccactc catgaatgga ctacatcttc tttagttatt ttaaacttga agtaggcatt
    22081 taccaattca attaaatagt caatttcttc ttcgccaatc tctatgtggt taagatctcc
    22141 agtatagtgg atatcggtgg ttccaatcat actaaagcca tgatagggga taacaaaaat
    22201 aacccgctga tcattatgtt gcaagaaata ggcatgattt ccctcaaaaa gtttggggac
    22261 gataatatgg ctacctttaa ccagggtaat atctttttgg ataggtgtat gagttaattg
    22321 ggcaatggat tgcacccatg gtcctgtggc attaatgatg cttttagcat aagctttata
    22381 gctctgccct ttggtgggct ggatagttaa atgccatatg ttattgatta cctcagcatg
    22441 ggttactttg gcatgtggcc ttatgcttgc gccatgattt ttggcttgta tggcattaag
    22501 aatagttaag cgagcatcat ccgtggtggc atcatagaaa agaaaaccgc gatttagctc
    22561 ctcttttagc ggcgtaaaat aattattctt attatttctg tggatcgatc tggatttggg
    22621 taaatgattt tttcgactga gatgatcgta aaaaaataaa cctaacctca ataaccatga
    22681 ggggcgcata tgttttaagt agggcaaaac aaaagattgg gaatggacta aatggggagc
    22741 caaattgaga agcgtttgac gttcctggag tgcttttttg actaaaccaa attcataatg
    22801 ctctaaatac cttaaaccac catgaattaa ttttgtgcta cttgatgagg ttttcgaagc
    22861 cagatcgtcc tgttcgaaga gtatcacaga cagacctctt aatgccgcat ctgcagcgca
    22921 gccgcagcca ttgatgcctc caccaataat tgcaacatca aaaacctgat ccatgttttt
    22981 atctccgaaa aaagattaca ctcaatatat ataaatatag agggatttta taataatgaa
    23041 ctatatacta gctattgacc agggaacaag tagcactcgc gctatgatct acacccttca
    23101 aggtgattta atcgcaaata gtcagtatcc tcttactcaa tattacccca atttgggttg
    23161 ggtagaacac gatcctgagg aaatatggca aaaaactctg aatgcgatga gggatgtcgt
    23221 atctcaagtt caaggaaaag caattttatg ctgtggcctt accaaccaac gggaaaccac
    23281 tctgatttgg aacaaaaaaa caggggaatg cctcgcgcca gctattgtgt ggcaagatag
    23341 acgtacggaa gagttttgtt attccttgtc tgaatactca tcaatgatac aagagaaaac
    23401 cggattactt cctgatccct atttttcagc gagcaaatta aactggttac taaagaataa
    23461 tgctcaagct caatcactaa ttaaccaggg ggatttggca tttgggacaa tagacagttt
    23521 tttaatatgg cgcatgacta aaggtgccag tcatgctacc gatatcacca acgcatccag
    23581 aactatgctg tttaatattc atgatgaaaa atgggataag cagttactcg agcttttttc
    23641 tatttcagaa tcgatattac cagaggttta ctccagtgat aatcattttg gggtgataga
    23701 taaagaatgg ctgggagtag aaataccaat taccggggtt gcaggcgatc aacaagccgc
    23761 tttaattggg caaggttgtt ttaatggcgg aatggttaaa gctacttttg gtacgggggg
    23821 atttttattg ctcaatacag gtgataaacc ggttttctca aagcataaat tgttgactac
    23881 tattgcctat aaaattaaag aaaaaacagc ttatggtttg gagggtagta tttatcatgc
    23941 cggaacaact gttaaatggt tacgcgatga aatgaagttg atatctcatt ctgctgaaac
    24001 tgaagtattg gcacaaagtc ttgatagcaa taacggtgta tatcttgttt catcttttac
    24061 tggtcttgga gcacctcatt ggttatcaat aacaggaacg gctattattg gtgtatcccg
    24121 ttcaacgaat agagcgcatt ttgccagagc cgccctggaa ggagtttgtt atcaaacacg
    24181 tgatgtatta acctgtatga gagatgatag tcaattggat ttgactttat taagagcgga
    24241 tggagggatg gcagtaaacc aatggtttct acaatttatg gctgatcagt gtcagttgac
    24301 aattcaaaaa cccaaggaca ttgaaacgac tgccaaaggg gcagcagtat tagccgcaat
    24361 aggttctggt ctatttgatt cagtttcttc agtaaataag gtatgggacg ttgagaacga
    24421 gtttcagcca tccagggaaa gagaggtagt tgaaaaagat tttcgtggct ggttagaggc
    24481 gttggcaatg gttaaagcaa ggcatgcata gactagtctt atatttaaat tggtgcgatt
    24541 aaattagatc gcagtagctc gtattatagt ttaatacaag ctactgatta acacattgga
    24601 ttttttattt tacgtcacga attttttcac cagtatacaa ttggcgaggt ctgccaatgc
    24661 gtgacttggt ggctaccatt tccatccagt gactcatcca tccaacagtg cgggctagag
    24721 caaatatcac tgtgaacata ttggttggta ttccaatagc gcttaaagtc aatcctgagt
    24781 aaaagtctac attcgggtag agttttttct caatgaaata ttcatcttca agagcaatac
    24841 gttcaagttc cattgctaat ttaaataaag gtgcttcttt tgctccaacg gcttctaaca
    24901 catcataaca ggtcttacgc attacttttg ctcttggatc atagctttta tagaccctgt
    24961 gcccaaaacc catcaaacgg aatggatcat ttttatcttt ggctcgttta atgtaatgat
    25021 cgatgcgact cacatcgcct atttctttta gcatatttaa gcaggcttca ttcgctccac
    25081 catgagctgg tccccataaa gcccctatac cagctgaaat acacgcaaag gggttagctc
    25141 cagtagagcc tgccagacgt actgtcgatg tagatgcatt ttgttcatga tcagcatgta
    25201 gaataaaaat agtatccata gctcttacca atactggatc tggcgtagta tcttcggatg
    25261 gaacaccaaa catcatgtga agaaaatttt ctgcataaga cattttattt ttaggataca
    25321 tgtagggcat accaatggag tatttataac tcattgcggc taaagtaggc attttagcaa
    25381 ccagtctgat tgcggaaata aaacggtctt gtgcgttatt caaatccatg gattcatgat
    25441 aaaaagcaga caaagccccc acaattccaa ccattatagc cattgggtga gcatcacgcc
    25501 tgaacccatt caaaaactgg tacatttgct gatgaaccat agtatgattg ttgatcagac
    25561 taacgaaagc tttcttttct tctttgttag gtagttctcc attgagtaat aaataactta
    25621 catcaaggaa gtcctttttt tctgccaatt gttctatagg ataaccccta tacaacaaaa
    25681 tgcctttatc cccatcaata taggtgattt ttgattcaca ggaggcagtt gataaaaaac
    25741 cttgatcaaa ggtaaagacg ccatgtgaac caagctgggt aatatcaatc acatcattac
    25801 ctaaggtggg agtatagatt ggtaaatcaa tcgtctcttg cccatcgatg ctgagtttcg
    25861 ctgtcttttg agtcattgct tctcctgatt atttacatgc tttaagcatt catacttgcg
    25921 ttagtgcgcg gaactatata aaaatgacaa catacagtca aattaaacgt aattttggtc
    25981 attaaaaatg ctagtccaaa atctgacgtt agttatacat tcaaataatt tagatcattt
    26041 gcaaccgtat acattgatta tatattatgg tttacaaatt tgaggaataa tagaaaaaaa
    26101 aatgataact ccagctgaat tacctattaa tcatcgtggt gcggtatatc atcttgattt
    26161 aagaactgaa gaaatagctg acctcatcat tacagttgga gaccctgggc gagtgagctt
    26221 ggttagtcaa cattttgata aaattgaagt gcaacgctcc caccgagagt ttgttaccca
    26281 tacagggtat ctcggaacta atagaattac agttctctca actggcattg gcatgccaaa
    26341 catagatatt gtgatgaatg aactggatgc tttggcaaac atcgatttat ctacacgaac
    26401 ggtgaatact aacaaaaaga gtttaacaat tattcgatta ggcaccacag gaggggtgac
    26461 tgcgaattgt atgcctggag atatagcgat tagtcgatat gctattggtt ttgatagttt
    26521 gattgattat tatcagtatc agccgatggg agtgcttgaa acaattaggg aagagttatc
    26581 tcagtatcta atgggagaga gtggcccatt ttatatttct gaatcttgcc cgcaattagc
    26641 tgaacagttt agtgccttag gccatacggg aattacagca acttgttgtg gtttttatgg
    26701 gccgcaaggc cgccagttaa gattgcctct tcattacccc aatcttatta ctcagttagg
    26761 aaaattttgt actcagaatt attcagtaat taatctagag atggaaaccg ctgctatcta
    26821 tgctcttggc agtatgcttg gacataaatg cttatcctta agtgtggtga ttaataacag
    26881 gatacatggt agttttgtaa ctgatgtcaa atcttgtctg gactccctaa ttacaaaatc
    26941 acttgcgaat attaaaaata tccttgagcc atatgaagct taaggtcatt taattaagac
    27001 gcccagtgtg gtatcctttt gcattaaatt aatcttaatt tattgtctaa aggatacatc
    27061 caaatcatgg tatacctagc caaagaagtc ttaccagtca acatagaaga cgaattgaag
    27121 caatcctatt tggattatgc gatgagtgtc attgtaggcc gagcgttgcc tgatgtacgt
    27181 gatggtttaa aaccggtgca taggcgagtt ctttttgcga tgagcgagtt gggtaatgat
    27241 tggaataaac cttataaaaa atctgctcgt gtagtagggg atgtcatcgg taaataccat
    27301 cctcacgggg atacagctgt ttatgacacc attgttcgta tggcccaacc tttttccatg
    27361 cgctaccttt taatcgatgg acaggggaat tttggctctg tagatggaga tgctccagct
    27421 gccatgcgtt acactgaagt aagaatgtcc aaagtggcgc atgctttact ggctgatttg
    27481 gataaggaaa ctgttgattt tagccctaac tatgatgaaa cagaatttgc tccagtggta
    27541 ttgccatcga gaattcccaa tttactagtt aatggctctt ccggtattgc ggtagggatg
    27601 gctactaata ttccaccaca taatcttacc gaagtaatca atgcatgtat tgccttagtg
    27661 gatgaacctg acacgagtct tgaagattta atggaaatta ttcctggccc tgattttcct
    27721 acagcagcaa ttattaatgg tcgtgctgga attattgaag gttatcgtac tggaaaaggg
    27781 cgggttgtta tcagggcacg aacagaaatt gaaacggatg aaagttcagg ccgtcagtca
    27841 attattattc aggaattacc ctatcaggtg aataaagcgc gtttgatcga gcgtattgct
    27901 gaattggtaa gggataagaa agtcgaggga atttccggct tgagggatga gtcagacaag
    27961 cagggaatga gagtagtcat tgaattaaaa cgcaatgaag tagcagatgt ggtattgaat
    28021 aacctgttcg ctcatactca aatgcaaaat gtattcggaa ttaatatggt tgctttggtg
    28081 gatggccaac cgcgtacttt gaatttgaag caaatactgg aatattttat aaaacatcga
    28141 agagaggttg ttaccagacg cacaatattt gaattgaaaa aagccagaag tcgagctcat
    28201 ttattggagg gcttgggaat cgccttagct aatatcgatg aaatgatcgc attaattaag
    28261 caatctccta ctcctcaaga cgcaaaaagt gctttattaa gcaaaatatg gcaacctggc
    28321 ttagtaaagg ccatgttgga aaaggctggc tcaaacgcgt caaggcctga tgatttaact
    28381 gaagagtatg gtttgcatga gaacggatat aaattatctg aagcgcaggc acaagcaata
    28441 cttgaattaa gattacatag gctaacagct cttgaacaag ataaaataat taatgaattt
    28501 gaagagctat tgaatttaat taaagaattg cttgacatat tggcttcacc tgaaagactt
    28561 atgcaagtta ttcgtgatga attgatagag attaaatccc aatttggtga tgagaggcga
    28621 acggaaataa cagcctcaca agaagatttg actattgaag atttgattac cgaagaagac
    28681 gttgtggtca ctttatctca tcaaggttat gtaaaatatc agcccattac agcctatcag
    28741 gctcagcgcc gaggtggcaa aggcaaatcg gcaactcatg tcaaggatga ggattttgtt
    28801 gagcgtttag tgattgctag tactcatgat accttattgt gtttctccaa tcatggcaaa
    28861 ttatattggc taaaagcata tcaattgcct caagcaagcc gcgcttcgag aggaaggcct
    28921 ataatcaata ttcttccttt ggcagaaggc gaagagatta atgctatgct ccctgtccgt
    28981 gagtataaag acggcagcta tgtgtttatg gcaaccaaga agggaacagt taaaaaagtg
    29041 cctttaaacg catttagcag gccgcgttcc aatggtatta ttgctgtgga cctggaagaa
    29101 gatgacagcc tcgtcggagt cgatattact gatggtactc gcgatattat gttgtttact
    29161 gatgcaggca aagtaatccg ttttgatgaa aataaagtgc gacctatggg gcgcacggct
    29221 cgaggggttc gcggaattcg agtagaaaag gatcaagccg ttaaatctct tgtggtagtc
    29281 gacccaaatg gaggcaccat tttgactgca actgaaaacg gctacggtaa gagaacccat
    29341 atcgatgagt atcgagtgtc aggacgggga ggacaaggtg ttatttccat tcaggttact
    29401 gaacgaaatg gtaaagtggt ccgttccttg caggttaccg ataatgacga agccatgtta
    29461 attacagata aaggaacttt ggttcgtttt aaagtcaatg aattatctgt gataggcaga
    29521 aatacacaag gggttcgcct gattaatgtc agttctggtg agacagttgt tggaatgcaa
    29581 aaaattgtag atcttggaga agaattagaa gaagcagaag attcatctct taatgccgaa
    29641 gacaacagtg atgaatagca gggttttcaa ttttggagct ggccctgcta tgctgcccga
    29701 ggaaatatta aaagaggctc aagaggaatt ccttaattgg cgtaatactg gaatgtccat
    29761 acttgaaata gggcatagga caccggagat cattaatctt ttaagtactg cggagcaatc
    29821 gttaagagag ctgttaaata tccctaaaaa ttatcatgta ttgtttttag gaggagcagc
    29881 tcgagcacaa tttgccatga tccctatgaa tttattacag ccaggggatg aggcggctta
    29941 ttttattact ggaatctggt ctaagatggc ttatcatgaa gcgaatcttc taaaacaagc
    30001 atattatctt agcaatgaag agaaagaggg atttgtatca attcctgatt atcaaaagtg
    30061 ggagttaaaa agtaatacag cttatgtcta ttatacccca aatgaaacaa ttaatggagt
    30121 cagatttcct tatgttccta aaacaggagg ggttccttta gtcgcggata tgacttcttg
    30181 cttgctgagc gagccgataa atattaatca atatggtttg atttttgctg gtgcgcagaa
    30241 gaacattgcc aatgcaggtt taactgtagt tatcattcat gaggatttac taaaaaacca
    30301 acctgaacca gtggttccta ccatgttgaa ttataaaaat catgccgaac atcgctcctt
    30361 atatgctaca ccaccagtat ttaattgtta tttggcaagt aaaatgttcg agtggataaa
    30421 aactcaggga ggtatagaag gattatttca aaggaattgt ttgaaagccg cgaagttata
    30481 tcaatatctt gattcaactg atttttatct aactccagta tctaaagaag ctcgttctat
    30541 aatgaacatt tgtttttccc tctgctatcc tgatttggaa caaaaattcc tcgatatggc
    30601 taatgagcgt gggttaaagg ccttaaaagg tcatcgtttc gcaggaggat tgcgcgccag
    30661 tttgtacaat gccatgccta tggctggagt tgatgcgctc attgagtttt tgtctgaatt
    30721 tgcaaaagaa aatggttaat aagcatgttg aattttataa gtaaaccagt tgtctgcctt
    30781 aagggcgaaa tcacagtacc tggagataaa tccatttccc atcgttctat tatttttggc
    30841 gcgattgcta ttggaacttc agtaatcgat ggctttttag atggtgaaga ttgcatagct
    30901 acccttaaag cattccaatc gatgggtgtt aggatagagg ggcctgataa gcaatgtgtc
    30961 attattcatg gggttgggaa atatggttta aaacaacccc aaaatattat tgattgtggc
    31021 aactcaggta ccagtatgcg cttgctggct ggattgcttg cagcacaaca atttgatagc
    31081 cagttaacgg gagacgaaag tttacttaaa cgcccaatgt taagagtcag tagaccctta
    31141 agtcaaatgg gtgcagatgt tactactcag gatgggaagc cgcctatagt aattaaaggc
    31201 ggaaaaaaat taaatggaat acattatgtt atgccggaag ccagtgctca ggtaaagtcg
    31261 tgcctgttgt tagctggtat gtatgctgaa ggacaaacaa aaataactga aaatgcagtt
    31321 agtcgggatc ataccgaaag aatgctaaaa acattttctt atccagttca gatacaagat
    31381 ggtactatca taattgatcg taatggcgag tgccgtggaa cttgtctgaa tatcccggga
    31441 gacatttcat ctgctgcttt ttttattgtt gcagcttcta ttactccagg ctctgatgtt
    31501 ttaattcgta atgtaggtat taatcctaca cgaactggca ttattcatat tttaactgaa
    31561 atgggagcgg acataaaagt tttgaatcaa cgtgcctatg gagaggaacc agttgctgat
    31621 ttgcatatca gatattccca attaaaagga atcgatattc ctgcctctat ggtgcctttg
    31681 gcgattgatg aattcccggt tatttttatt gcagctgctt gcgcacaagg gaaaacaact
    31741 cttcatggag ctaaagagct gcgcttgaag gaaagtgatc gtattggcgc gatggtggat
    31801 ggcttaaacc aattgggagt tcatgctgag ggttttgatg atggaatatt gatagagggt
    31861 ggaagtatac agggtggtga ggttaatagt cgaggagatc accgtattgc catgtcattt
    31921 gccattgctg gtgctgtcgc cagtgctcct gtaacgatta aaaactgcgc caatgttgca
    31981 acttcatttc catcttttgt tactaccgct aacatgcttc attttcagat agaggaatac
    32041 agctgatggt ttttaataat gaggttcctg tcattacgtt ggatggccca agtggaacag
    32101 gaaaggggac aatttgtcat ttggtagcca agagactgca ttggaatatg cttgacagtg
    32161 gtgctattta tcgtgttcta gcttatgctg ctcggaaaaa tagcatcgaa ccgaatgata
    32221 ttaaaaaatt aactgctttg gcccgttccc tgaatttgcg ttttgaatca tctgtggata
    32281 atgaaacaaa agtaatactg gatgatgaaa atgtcagcca gcaaatccgc agtgagcaat
    32341 gtggtcagga tgcctcacaa attgcagtga ttcctgaagt aagagcagca ctgcttgaac
    32401 gccagagaaa ctttgcccaa tttcctggat tggtgacaga tggacgcgat atgggtacgg
    32461 tggtctttcc aaatgctgtt ttgaaggttt atttatatgc aagtgcagaa gaaagagcaa
    32521 atagacgata tttgcagttg caagataatg gaattaatgt tagcctcgcc caggttgttg
    32581 aagaactggc taaacgggat gcaagggata cagctcgtac gcatgcgcca ttgaaaccag
    32641 ctgaagatgc tgtcttgatc gatacaaccg gactaacaat tgtacaagtg ttcaatattg
    32701 tattaaaatt aattgatgaa cgtttgaata atttgtagtt ttgaattggg gggaaggtga
    32761 gtggagctca ttcttccatt tttttactaa agagtttatt aacatgtctg aaagtttcaa
    32821 agaattattt gagcaaagta ttgccggagc tcaattttat cctggtgcca ttattactgc
    32881 taaagtcatc gatatcgatg atgattttgt tactctcaat gctggactaa agtcagaagg
    32941 tattgttgcc gtagaagaat tctacgataa aaatggcgaa ttggaagtta aagtaggcga
    33001 tactgtcgaa gtggccctgg attcagtaga agatggtcac ggcgaaacgc tcctttcaag
    33061 agaaaaagca aaacgccagg aagcatggcg caaattatcg aaatgccacg aaaataatga
    33121 aacagtcact ggtttgattt ctggaaaagt caaaggtggt tttactgtgg aaattggctc
    33181 aatcagagca tttttaccgg gttcccttgt tgatgttagg cctgtaagag atccatctta
    33241 cctcgaaggt aaagaacttg aatttaaagt catcaagatg gatttgaaac gcaataatat
    33301 tgttgtttct cgacgagctg tagttgaaga agaaagtagc gctgacagac aagcactact
    33361 ggagtcttta catgatggtc aagagcttca cggtattgtt aaaaacctta ccgactatgg
    33421 tgctttcatt gatcttggcg gcatagatgg tttgttacat atcactgaca tttcctggaa
    33481 gcgagtcaag catccaagtg aagtattatc agtaggtcag gatgttaaag tcaaagtact
    33541 aagctttgat agtgaaagaa accgcgtttc cttgggtatg aagcagttag gcaatgatcc
    33601 ttgggttgat ttggttgaac gttacccaat aggcaagaga ttgcaaggta aggtaaccaa
    33661 catcaccgat tatggttgct ttgtcgaaat tgaggaaggt gtagaaggtt tagtccacat
    33721 gtcagaaatg gattggacta ataaaaatgt tcacccaagt aaagtagtgt ccctgggaga
    33781 tgtagtcgac gttatggttc ttgaaattga tgaagaacga cgacgtattt ctttaggtat
    33841 gaaacagtgt gtgggcaatc catggcagca attcgcatct actcataata aaggcgaaaa
    33901 agtaaaaggc aagatccgtt ctattactga ctttggaatc tttataggtc ttgatggtga
    33961 tattgatgga ctagttcatt tatctgatat ttcctggact gttcctggtg aagaagcggt
    34021 aaaacaattt aaaaagggcc aagagttaga agctgttatt ttagcaatcg atccagagag
    34081 agagcgtatt tctttaggat tgaaacaatt ggaaggcgac agttttgcca gctttgctga
    34141 aacttacacg aaaggatcta ttgttaaagg tacagttaca gctgtagaac ctaaaactgt
    34201 aacagttgcg ttagcagaag atgtttctgg gacaattcgt gtgagtgaat tatccgatga
    34261 acgtgttgat gatgctacca ctatcgtaaa agttggtgat gaagttgaag caaaaatcac
    34321 taatattgat agaaaaaatc gcaccatttc actttctgtc aaggcaaaag atgcgcagga
    34381 tgaagcggat gcaattaaga agtactccag aacagaggca gcatcaacta ctctgggtga
    34441 tttattgaaa gaaaaaatgg ctagcaaaga aggtgaataa cctttttaag tcaaaactaa
    34501 aaaagcgtag aactatctac gctttttttt attttgagaa aaagattatg gtatgatgga
    34561 cgttttagct taaggattaa aggaaatatt caatgcgcat attaatgctg gtaacttatt
    34621 tgctgcttat tattattggt gttagttttg cggctctaaa tgcctcgtcg gttcaagtta
    34681 atttctattt taaaacggtg tccatgccta tctctgttct tatgactatc atgctcggag
    34741 ttggtgtttt tataggtttt gttctcttta ttacacgata ttggcgttta aaaattgaat
    34801 gtcgtaaaat gaaaaatcaa ttaaaactaa ctgaaaaaga aattaagaat ttacgttcaa
    34861 tacccttaca agatcaacat taattttatt attagtggaa actcgatacg cagagaggaa
    34921 aattaataga tgattaattt atggccactg ttgttacctg ctgccgcatg gtctggctgg
    34981 tggctggcta accgcagtaa ctccaataaa gatgctcatg tccacaatcg cttatcgcga
    35041 gaatatgtag ttggacttaa ttatttatta aatgaacaat ctgataaagc ggttgacgta
    35101 tttatcaagc ttctagaagt tgatagcgat acagtagaaa ctcatcttgc actgggtagc
    35161 ttgttcaggc gtagagggga agtagacagg gctattcgta ttcatcaaaa tttaatcgcc
    35221 agacctcaat taagtattca acagcgaaag gaagccctaa tggctttagg gcaagattat
    35281 atgagtgctg gagtgtttga ccgagctgaa cgtatattcc ttgaagttgt tgagttgggc
    35341 gggagtagag atacaagcag tcttcaagga ttattagcaa tttaccaaca agaaaaagct
    35401 tgggaaaaag ctttagatat tattaagaaa ctggaaattt ctaccggagc cagtttccac
    35461 aatcaagcag ctcattatta ctgtgaaatg gctagtcaag cgcttaaaat caatgcaatg
    35521 gatagagcaa tttattgtat aaagcaagca atgaatgttg atccggagtc cgtaagagcc
    35581 agtttgatga atgctacaat tgaaataaaa gaaggccgct ataaacaggc tattcgatct
    35641 ttaaaaagag tgccgcagca ggatgcagag tttttaaccg aaatcattga accattagtt
    35701 ctttgtcata aagagctgga ctccatgccg gattgtattc actatcttga gcaaactttg
    35761 gaaaaacatc cgcgagcctc tgtaattttc gttatcgctg attatttgcg acaaaataag
    35821 ggtatggata ttgcaattga ctttgtggct gataatttaa gcaaacatcc atcgataaga
    35881 ggcttgaatc gtttgattta ttggcacttg gagtctgctc atgggaaagt acgtgaaaaa
    35941 ctgcaaatgc tttatgatat tactagcaag tttttagata ataagcccat atatcgatgt
    36001 gggcattgtg gttttggtgg taaacattta cattggcatt gcccaagttg taaacagtgg
    36061 ggtagaatga agcctatcca tggtttggaa ggggattaac ctgagacgta ctctcagaaa
    36121 gttaatgaac agttgcatat tagcttagta tcgtaattca aatttgttat tgcaaattaa
    36181 gtaaaacaat tcaaatagga aatttgattt gttttgtctt atcgtttgcc aaggcagttt
    36241 acgttcaaat cagtttattg caactttaga tttgtattaa gattattcct taacttcatg
    36301 gtattgatct taggtatagc taaacaattt atatactaat tatgagatta taatgcaatt
    36361 tatcgactta aaaacacaat attctttaat tgagaatgat attttaaaca gcattaaaag
    36421 ggtattaaat catggccaat atattatggg gcctgaaatt gcagaacttg aaaaagagtt
    36481 agccactttt gttggagtga agcatgcgat tgtgaattca agtggtacag atgctttatt
    36541 aatggcattg atggctctgg atattcagcc tggggatgaa gtaattacaa gcccatttag
    36601 tttcttcgca acagcagaag tgattagcct ttgtcatgca acacccgtct ttgtagatat
    36661 agatccttta acttataatt tggatcctga gcagttagag agagcaatca ctaagaaaac
    36721 caaggccatt atgccagttg atttatatgg gcaatgcgca gattatgatg tgattaatgc
    36781 cattgcaact caatacggta ttcctgttat tgaagatgct gcgcagagtt ttggagctac
    36841 ttataaaggc aaatactctt gttccctggc tactattggc tgtaccagtt ttttcccatc
    36901 aaaaccttta ggtggttatg gtgattctgg agcttgtttt actaatgatg atgttttagc
    36961 ccaaaaatta attgaaatta gaattcatgg acaaaatgcc cgctattgcc atcatcgggt
    37021 agggattaat ggtcgaatgg acactatcca agcagcaata ctacttcaga agctgaaaat
    37081 attttctaat gaaattctat tgcgacaaaa agtagcaaaa cgttatgatc aaatgctttc
    37141 tgagttagtg aaaactcctt atgtgcatga atgcaatgca agtgtgtatg ctcagtatac
    37201 cattgaggtt gagaatagag atgtatttgc gaagtcaatg caagcctcag gtatacctac
    37261 agctgttcat tacccaattg ctatgcatca acagcaggca cttggttact taaattacaa
    37321 acttggagat tttccaaact ctgaaaaagc aagtcaacat gtaattagcc tgcctatgca
    37381 cccttattta caagaagatg agcaattgaa aatagttgca gcagttagaa atgctttact
    37441 tgtgatgaat gaagaggcca tggtataaat gacacctaaa ttaatcgttg cgttggattt
    37501 tgataatcaa gataacgctt tacagttggt tgacaaactt gatccgaatc attgcgcact
    37561 taaggttggg agtgagcttt ttactctatt agggcctcag tttgtaaaag aattggtaag
    37621 aagagagttt aaagtatttt tagatttaaa atttcacgat atccctaata cagttgctaa
    37681 agcatgccac tcagctgcag aattaggggt atggatgata aatgttcatg ccatcggtgg
    37741 cttaaaaatg ttgcaagctg caagggaatc tttaaaaata tatggtaaag acaggccttt
    37801 gttgattgca gtgacagtgc tgactagttt tgaagaaggg gagctggcca gtgttggtat
    37861 tagtaataca ttgcctgagc aagcaaccca tctggctatg cttgccagag aggctgggct
    37921 tgatggtgtg gtaagctctg cgcatgaagt taaaattata aaacagaagt gtggtgagaa
    37981 ttttattacc gttactcctg gcataagact gccaaataat ctaaaagatg atcaatcgcg
    38041 agtaatgact ccacaacaag ccatcagaga aggaagtgat tttctggtta ttggaagacc
    38101 aattacgcaa gcgagcaatc ctcatgaggt tgtctcagct ctattgcgtg atctttgatg
    38161 gcacatactt ttaaaaaatt atacatatgg tataattttg ttcatttttg aattagttgt
    38221 accattccta ttttattcca tagaaattta tgataattgt tataaagatg attaataaat
    38281 atttaaaaat aatcaatagt atttcagata tttattctat ggactaccca gacgtaagga
    38341 tttactgatg accccacagc acgtcataag tcaactgctg gaatctgaca ccaaataccc
    38401 tcagaattta gattt
//
LOCUS       DASLOE010000013        37531 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.013, whole genome shotgun sequence.
ACCESSION   DASLOE010000013 DASLOE010000000
VERSION     DASLOE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 37531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37531
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.013"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            106..1278
                     /locus_tag="U6309_000523"
     CDS             106..1278
                     /locus_tag="U6309_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5503978.1"
                     /translation="MVGVFFLLFQFFLQLSSGIIVGAIMHEEHISALSAGLLSSAFYY
                     VYTSFQIPVGILFDRYNTRYLLSVNATLCAIGCFVFASGHNLPTLFLGRLIIGAGSAF
                     AFIGLSHLLRQHFPLKQYAFMIGLSETLGFTVTVLGMIGMGSVISHFGWRYFINIAGI
                     IGFLIASLCWLCIPNSKPVVSFPHQYKQQIIPILKNKLAWFNGLFVCLEFSVITVFAA
                     MWAVPFLQLKLDCTIKTASILTSMVLLGAGLSCPLLGQISVHLNRRKPLIHASCLSTA
                     LLLILVLYLPTQNKLLIGILMFSMGLTCGAYMLAYSISNELAPPEFLSTCTGFTNTLA
                     MLSAPLLQPLVGFFLDVLSNHKSQYSLADYQLALLIVPFAMLLASLFTCLLPEKSN"
     gene            complement(1280..2230)
                     /locus_tag="U6309_000524"
     CDS             complement(1280..2230)
                     /locus_tag="U6309_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503979.1"
                     /translation="MMPKRIWLLLLSMFLFLSGSVFAVEEPEEDKVAQTNEPTVGSWV
                     FTGMVTNESGDRYGYFFQMQRQGTDFYAKTALIDGQTNQLILFYEANEKIDSSTQLNW
                     QVGRSFLRYNPINDSWIFGVKVENAKGFNFKVDMLKQDNKDVETQVLRPGVELQAVQT
                     SRLNGHVQTGVDNKEQFVTGNKAWFSKLWFSKDQNSPHDISTIFCRLSNDNGFYSANL
                     KEPDATGAAVAGWRDPSGNNVKMSQFVSIKTLTDNQCLLSVGLPRLKLKLVNTIKQED
                     DFPLTVAGFSEENPKGFCFISKQTFKQIKESAVQAVTNLG"
     gene            complement(2232..3203)
                     /locus_tag="U6309_000525"
     CDS             complement(2232..3203)
                     /locus_tag="U6309_000525"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEN5503980.1"
                     /translation="MSFFVNRIWYGNHFLQWILVPFSWLYRIVIRTRRWYLQRFCQQL
                     YPIPIIVVGNVTVGGVGKTPLVIEIAKKIQQKGLKVGIVSRGYKAAIKHFPYEVKLND
                     SAELVGDEPLMMARKINCPVVIAPKRNEAVRYLLDKHSVEIIISDDGLQHYKMGRSIE
                     IVVIDGMRKLGNGFCLPAGPLREPDSRLKQVDFVIVNQGAAEGTYSMELIPKNIVRLS
                     TQEEVSNDLFTSEVAAVAGIGNPQRFYSTLSQLGIKFNPYSYPDHHQFKPHDLNDIDL
                     PVIMTEKDAVKCYSFSSDKLYYLPVEAKLNDSFWEAFWSHQQLQGYY"
     gene            complement(3203..4969)
                     /gene="msbA"
                     /locus_tag="U6309_000526"
     CDS             complement(3203..4969)
                     /gene="msbA"
                     /locus_tag="U6309_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEN5503981.1"
                     /translation="MKNNLPIKSRLLYKRLLSYVKPFWPVLLLGVLANILYSGIDAGF
                     TYMTKLFLDKSFITIDLNFVKQIPLIVLIGITLRGLVSSLGSYCMTWVARSVVKVLRQ
                     TVFSHIIHLPADYYDEATSGQLLSKILYDVEQVAQVSADALTDFIQNICLVIGLLTVM
                     MVICWQLSLMFLLTIPFVGIIVNYTNKRVRRISHKVQKTMGEVTEIASEAIEGYRVVR
                     IFGGERYEITKFNKATEYSRKNDMKVAISKAINVSGVQLVIAIGIATIIMAAIHLSTV
                     ITISAGSFLAIIAAMLQLIKPMKTLTTLNATIQRGLAGAESVFNLLDLPLERNNGLIL
                     KEKIRGEIEFKHVYHAYRQGQNILHDVNFVIEAGTSVALVGHSGSGKTTIASLLPRFY
                     ELSQGMITLDGMPIQQLSLESLRKQMSLVSQNVTLFNDTLANNIAYGRFDASREQIIT
                     AAKLAYADEFIKQLPDGYDTRVGENGVLLSGGQRQRIAIARAILKDAPILILDEATSA
                     LDSESEHYIQAALEQVMKGRTTLIIAHRLSTIKHAHKIIVLQHGRIVEQGSHQELLDM
                     DGHYAQLYKVQQFGRINEEVVA"
     gene            complement(5056..5838)
                     /locus_tag="U6309_000527"
     CDS             complement(5056..5838)
                     /locus_tag="U6309_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5503982.1"
                     /translation="MYITKELAVRMEGCIKQSHIEVTKRYSQGKILEINGGAACFSGF
                     ESFLSQVVGWGFKTEPNQYKAEIEAIENFYKSLGHNRVDIELCPFVGNHLINFLSQRG
                     YYITELNNVSVLDLKSYHLVAHSEDDGFIIREVKQSELEEWAKRVAIGFGFLEAQEQF
                     YQYASAKGVLAFAAYDHGNLVAGATIAIHGDACDLGVTSTLPLYRGKGLQKKLLFARL
                     NLAKQYGLEFATVTTQPGTVSDLNVQKAGFRCAYTRIKLTME"
     gene            complement(5859..6860)
                     /locus_tag="U6309_000528"
     CDS             complement(5859..6860)
                     /locus_tag="U6309_000528"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEN5503983.1"
                     /translation="MYSLLRPLLFRLDAEKAHSLTLSLLHYLPGFYFRKIAGQPVHAM
                     GLVFPHQVGLAAGLDKNGEHLDALAKLGFSFIELGTVTPKGQAGNPKPRLFRIAEANA
                     IINRMGFNNSGVDVLVENVKRANYKGILGINIGKNKETNLNQAAEDYLYCFRKVYDHA
                     SYVTINISSPNTPDLRQLQQGDYFAELLTQLQKEQIKLADQYGRHVPLVVKVSPDETD
                     ETLKQMTDIILQYGIEGIIATNTTCSREMVKNLPCSEEQGGLSGRPLMELSTRCLRLL
                     KQYVGNDVTLIGVGGIDSLESAKDKINAGASLLQVYSGLVYKGPELIHDIVSGLNAI"
     gene            complement(7146..8144)
                     /locus_tag="U6309_000529"
     CDS             complement(7146..8144)
                     /locus_tag="U6309_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503984.1"
                     /translation="MAVSNKKNSEFTPSLRSHMIAALLSMGAAAIILWLASGMTLAAV
                     IVDSFSLGFYIGFMLLMSAAPAVLASPVILPGLLLVVSGLFLGAVAATFAHRLKNNPS
                     LPQNNNGNGLINELTKDKSELLISHDDLQCQSNSLEGENTLSQQNEDNVTGEKFGFMK
                     FWGSLGLTSFASSGATALLGWLATDFVVSLSIIPVFLLGMTLAIVLVPLSIGLSGIFA
                     LPAVTALMFALGGFATGVFAATLAAYFIADNQAQDHPVEPIIRSSMERQCKDQGSHQK
                     ILNGLKKDKLSANEEVINSDLNEQKSRGPKTSKSDTGVKEEHINTAEEYSFCGLRF"
     gene            complement(8193..8879)
                     /locus_tag="U6309_000530"
     CDS             complement(8193..8879)
                     /locus_tag="U6309_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503985.1"
                     /translation="MRKLVLWGHGIEDYREMFDLPQGVENSRLLEYGCGPSAVNYQQT
                     QVKKNGVVSCDPLFVLDKDTLLAKAKMIFANMAEEVRQHQDQFDFSRNGSLDKLLQER
                     QEGMKQFFADYEQGKADGRYLGLTDYHLPFADFTFDFALSSHYFFADLDDQTVDFHLI
                     AIRELARVAKEVRIFPLIDREGNTSQFLGPVLLGLQQENYGVEVREAAFHLHKSGNAM
                     LRVWAQQCVV"
     gene            9214..10374
                     /locus_tag="U6309_000531"
     CDS             9214..10374
                     /locus_tag="U6309_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEN5503986.1"
                     /translation="MYTGLDFQLGETYDMLRDSVNQFAQTEIAPLAAQIDEKNTFPNH
                     LWRKLGEMGLLGITVSEEYGGANMGYLAHAIAMEEISRASASVGLSYGAHSNLCVNQI
                     FLNGNNEQKQKYLPKLISGEYIGALAMSESNSGSDVVSMQLQARSAGDKYILDGTKMW
                     ITNGPDADVLVVYAKTDKQAGSKGITAFIIEKGMPGFKTAQKLDKLGMRGSNTCELVF
                     DQCEVPSENVLGTVNQGVKVLMSGLDYERTILAAGPVGIMQACMDVVLPYVHERKQFN
                     QAIGEFQFIQGKLADMYTDLNACRAYLYAVAKACDNGRVSRKDAAGVILYTAEKATQM
                     ALQSIQILGGNGYINEYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFKETA"
     gene            10386..11570
                     /locus_tag="U6309_000532"
     CDS             10386..11570
                     /locus_tag="U6309_000532"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEN5503987.1"
                     /translation="MEHNDVVIVAAKRTPMGAMLGNLSALSSPELGAVAHMAALSQSG
                     IAPAEIDEVISGCVLQAGIGQAPARQAAIKAGIPNSTGATTINKMCGSGMKAVMLAHD
                     LIKAGTANVILASGMESMSNAPYLLSKARAGYRLGHGELKDHMFLDGLEDAYDKGQLM
                     GCFAETTAKHFNFSREQQDEYALRSMTKALKAIENGAFREEIAPVTLTTRKGDITVDV
                     DEGPETAKLAKIPQLKPAFQADGTVTAANSSSISDGAASLILMSASNAEKRGIKPLAK
                     IIAHASHSQAPQWFTTAPVDAIRKVMNKASWKQDDVDLFEINEAFAVVAMAAITQLEL
                     NPEQVNIHGGACALGHPIGASGARILVTLMHALKHQGKKRGIASLCIGGGEATAMAIE
                     LI"
     gene            11708..12022
                     /locus_tag="U6309_000533"
     CDS             11708..12022
                     /locus_tag="U6309_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503988.1"
                     /translation="MLRHSLIYLLLSILIVVFAKYAHLIVVYIDMFFTYVNLKLTPIF
                     SQTGWGLIIRKILVLMLLPIIIAGIPALIYRALKGKDMPHFIAIVWIIWTIIVLSDIL
                     IR"
     gene            12032..13639
                     /locus_tag="U6309_000534"
     CDS             12032..13639
                     /locus_tag="U6309_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylcrotonoyl-CoA carboxylase"
                     /protein_id="HEN5503989.1"
                     /translation="MAKLTTQINTSSQEFKNNQANMQALVTDLREKIHQISLGGDEKA
                     RTKHQQQGKLLPRERLHQLLDPGSPFLELSQLAAYQVYEDTIPAAGIITGIGRVAGNE
                     CVIVVNDATVKGGTYYPLTVKKHLRAQEIALINHLPCIYLVDSGGAFLPLQDQVFADK
                     EHFGRVFYNQAQMSALNIPQIAVVMGSCTAGGAYVPAMADESIMVKNQATIFLGGPPL
                     VKAATGEVISAEELGGAEVHCRHSGVSDHYAENDAHALHLARVAISNLNRKKPDSIHR
                     VDTVPPLYDSEDLTGIIPTDPRKPFDIREIIARVVDGSEFDEFKALFGTTLVCGFARL
                     YGYPIGIIANNGILFSESAQKGSHFIELCCQRKIPLVFLQNITGFMVGSKYEASGIAK
                     HGAKMVTAVANANVPKFTIIVGGSFGAGNYAMCGRAYAPRFLWAWPNARISVMGGEQA
                     ANVLAQITREKYAKQGKEWSLEEEEQFKTQMRSQYETQGNPYYASARLWDDGVIAPQD
                     TRKILGLGLSAALNAPIEDTRFGVFRM"
     gene            13659..14453
                     /locus_tag="U6309_000535"
     CDS             13659..14453
                     /locus_tag="U6309_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEN5503990.1"
                     /translation="MSDLLYEIQDKVGLLTMNRISKHNAFDNQLLTEMRIRLDSAIND
                     TNVRVIVLKANGKHFSAGADLTWMQSMANFTEEENLEDSLVLGNLMYSLSQSPKPTIA
                     MVQGSAFGGGAGLAAACDIAIASTSARFCFSEVKLGLIPAVISPYVVRAIGERAAKML
                     FMSAEVFDATRAYSLNLVQHCVPDDTLLEFTLKYASQISNNAPEAVKNSKQLAQYVAN
                     KKIDEELVRYTASLIAHKRVSDEGQEGLKAFLNKEIPNWNKGSGHV"
     gene            14446..16410
                     /locus_tag="U6309_000536"
     CDS             14446..16410
                     /locus_tag="U6309_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEN5503991.1"
                     /translation="MFNKILIANRGEIACRIIKTAHSMGIQAIAVYSAADRNSLHVRL
                     ADSAYYIGEAPAKESYLNIDHIIQAAKESGAQAIHPGYGFLSENPDFAKACEQAGIVF
                     IGPSIKAMEAMASKQLAKQLLEKTKVPLTPGYHGVEQSEEKLLSEAKKIGFPVLIKAA
                     NGGGGKGMRAVHDEKEFHDALAGAKRESMASFADDTMIIERLVLNPRHVEVQIMADNH
                     GNVVHLFERDCSIQRRHQKIIEEAPAPNLSPMLRQRLAEAACEVARSINYRGAGTVEF
                     LVDGEDKFYFMEMNTRLQVEHPVTEMITGLDLVAWQIRIAANDTLPLLQNQIQAQGHA
                     IECRIYAEDPYQGFIPSIGQLQFLKEPSGDGIRIDTGVTLSSEITRYYDPMIAKLIAW
                     GHNREEALQRLERSLSHYDIGGVKTNIPFLRAICRHVKFKEAKLSTDFLEKENINLPK
                     PDNELGILLAISCDYLGMVNRTTDPLLQDAFGWQMHLSSHWVWRYQLNSTIIEAQITP
                     IDSKKFKAKIENKEMVIHARYDLDQLIIEIDQKSVKARVENKEHHLIFYTDKGQLSIE
                     RFYWSKLDAQTSAHKGQLTAPMPATVVAILKNIGEQVKAGESLIVLEAMKMEHTIHAP
                     IDGILSDIFYSVGSQVSEGAELLALSESDT"
     gene            16421..17329
                     /locus_tag="U6309_000537"
     CDS             16421..17329
                     /locus_tag="U6309_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEN5503992.1"
                     /translation="MDYPQHVTIIEVGPRDGLQNEPSFLPSDKKIELINLLSQTGLKE
                     IEVTSFVSAKAIPQLADNEEVFQSINKTPSINYSALVPNERGMLKALEMGVQNIAVFT
                     AASELFNQRNINCSIKESIERFKPVLALAKTNQIRVRGYISCVLGCPYEGYIQPSQVV
                     SVTKMLLDLGVHEISLGDTIGVGTPRQTQLLLDAILPILPITQLAMHFHDTYGQAVAN
                     IYASLEYGVNRFDSSVAGLGGCPYARGASGNVATEDVLYLMHGLGIDTGVDIFKIVAA
                     GDMICKALGRKNQSKVANAMLANPCN"
     gene            17486..19426
                     /locus_tag="U6309_000538"
     CDS             17486..19426
                     /locus_tag="U6309_000538"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEN5503993.1"
                     /translation="MNDKVWTPKNPEQTRMWQFMDFAAKRHCQVFENYQDLHTWSVKH
                     PESFWETLCLFFKLNFDTPPHQILNYYSEMIEARWFSGARFNFAEKLLTRRDNHPALI
                     SLDENDSKSVISYAELYEKVAQCAAGLKAVGVATGDRVAAVMPNTSYTIIAMLAATSL
                     GAIWSSCSPDFGTQAALDRIGQIEPKVLFICDGHQYQGKKHSSSEKVVQLTAAITSLR
                     QVVICPNINEEINLEQLPNASYWNDFIKPEKECDFISLPFDHPVYILFSSGTTGKPKC
                     IVHGAGGTLIQHLKELGLHSDIKSTDNLCFYTTCGWMMWNWTVSALALGATITLYEGS
                     PTYPTDNRMFRLIDEEKVTVFGTSAKFISAIEKAGVKPKSEFQLSELRCILSTGSPLL
                     PKNYDFVYEHIKKDLQLSSISGGTDIISCFALGNPILPVYRGELQCIGLGMEVAVYDE
                     QGHSIKQKRGELVCTKPFPSMPVCFWNDKDRQAYKHAYFERFPGVWAHGDFAEITAHN
                     GLIIHGRSDAILNPGGVRIGTAEIYRQVEKVDDVVDSIVIGQDWQDDVRVVLFVKLRE
                     GKKLDDELINLIKTTIRKNASPRHVPAKILQVPDIPKTLSGKVVELAVRQVVHGQSVS
                     NLQSLANPQALEYFKDRDELKG"
     gene            complement(19596..20012)
                     /locus_tag="U6309_000539"
     CDS             complement(19596..20012)
                     /locus_tag="U6309_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK family protein"
                     /protein_id="HEN5503994.1"
                     /translation="MKKMFLAVFTLCSFTAQAAVELKTFQNIVDAIAEGKRITFVINL
                     KKCTSEMPLNSAIVSVTPNAVMVVGDSRVTASDRHFTLDDPVARGTPMFDYSKFNLDS
                     EGDASIKTTVLNASSYERLGSYQMNCKLGDGFKVFG"
     gene            20465..21490
                     /locus_tag="U6309_000540"
     CDS             20465..21490
                     /locus_tag="U6309_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEN5503995.1"
                     /translation="MIELCGLKKSFSGKLALNDINLFIQEGEIFGVIGKSGAGKSTLL
                     RCINILEKPDEGEVIIDGQNLMSLSRKDLALARHKIAMIFQHFNLLNSKTVFDNIALP
                     MRIQGIDEESIKQKIEELLPVVELTDKKDAFPSQLSGGQKQRVAIARALSCSPKVLLC
                     DEATSALDPATTDAILSLLKKINELYGITIVLITHEMDVVKRICQRLSVMVDGKIVET
                     TALSNIFNKPESLARKMLYAQISPELPTCLTRRLADYATEKPLVRLFFQGEEATVPFI
                     SQTSRELNMDINILLANIDRFDGVTCGVLVVELTANPLLLEAFINRCEQAGITVEVLG
                     YVLPDGL"
     gene            21468..22115
                     /locus_tag="U6309_000541"
     CDS             21468..22115
                     /locus_tag="U6309_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN5503996.1"
                     /translation="MSYQMVYDLLSATGETLYMVFFSTLFAVLLGLPLGILLYSSSRI
                     KPNIKLNKILSALINIFRSIPFIILLVAIIPFTRLIVGTSIGINAAIVPLTVGATPFF
                     ARLVDNVLQSLPLGLIETGYSMGANTRQIILHIILPEAQSGLIHSITVTAITLINYSA
                     MAGAVGAGGLGTLAINYGYQRFNAGIMIATVIVLIILVQLIQMVGDYLAKRCTHY"
     gene            22128..22907
                     /locus_tag="U6309_000542"
     CDS             22128..22907
                     /locus_tag="U6309_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN5503997.1"
                     /translation="MRILSCLVLIISLVACSSKPAPNTLVIGTIAGPETELIETAKQV
                     AEKEYGLNIKIVEFNDYNLPNEALQDGSLDANVYQHLPYLKAAILSHGYDLQAIGRTF
                     VYPMGIYSKKYKTLSELPENGIIAVPNDPSNEMRAFLLLEKAHLITLKNTTNSGIQDI
                     ESNPKQFKFKEIDAAQLPRVLPDVDAAVINTTFALPAGLSPSKDALFTEGKDSPYANI
                     IVIRRDTEKRPQLELFVKALNSEEVKEKAKKLFGEDAIPAW"
     gene            22987..24405
                     /gene="ceg25"
                     /locus_tag="U6309_000543"
     CDS             22987..24405
                     /gene="ceg25"
                     /locus_tag="U6309_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg25"
                     /protein_id="HEN5503998.1"
                     /translation="MAFYNSIPLIGKHLKSESRAKKYTSEEFKRLERKYRDHIGLPEN
                     SSFSSDAFDKLVSSRVDDFRAFPKTSLPGGLLFHDKLKEANTTEGENIRDVLSKTVLA
                     PTEKFKTAQKNFTESTSTFKELTKLIPSKFRAQDLVGTMKELTDDARKAIIEQQAHEI
                     QMLKEKLDPQKEGQAGNYVSDFKKALGLKDNDEVKKVTEGLIKELEETHKKQLGEFDK
                     STSDSLNQLHKASASESSEYLFLANLYENNKEMRQTLEKLYAKERQRKGLPPESTTVQ
                     VGISENKIDLNGIKLKDIPVIKSITGREIHQQPDGSFTIQMPMINPRYYQDPRQNVKT
                     DMMLIAQAIKASGYDSIEMNCNFPNSEKVAMERGRQAFAACIEAGFPVDKITINVNGK
                     PMKADELFKEHPKTYQDIMSRASKISKDYEDIKAPKTSTAPVNTVAVKQEIATLRAKQ
                     QQEERAQTQPVDEQRHGMTPNN"
     gene            24686..24853
                     /locus_tag="U6309_000544"
     CDS             24686..24853
                     /locus_tag="U6309_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5503999.1"
                     /translation="MSTRRFDEFDNEMDDFSDAEPVITEASPSTKLERRRKIEDLLEE
                     KRLREELEEFG"
     gene            complement(24850..25581)
                     /locus_tag="U6309_000545"
     CDS             complement(24850..25581)
                     /locus_tag="U6309_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5504000.1"
                     /translation="MNRIKAVGYENNELKEKAQLLADQLNLQLDQNADTCLFVTPEKL
                     TLKIRNFSLMFADFSTMTWSKRRGEGKKQGLIRACKPTKGIKILDATAGWGKDAAILA
                     TFGADVLMLERHPVMAALLADALSRRNEADIQKMCLSLIASDAISFLHSLQEKDYPDI
                     IYIDPMHPERNKSALVKKEMQVLQQLIGTDYDAMELIKLSLSHVKSRVVVKWPQKVKP
                     LLPPDASIDGKTVRFDIYMPQFSSN"
     gene            complement(25584..26114)
                     /gene="gmhB"
                     /locus_tag="U6309_000546"
     CDS             complement(25584..26114)
                     /gene="gmhB"
                     /locus_tag="U6309_000546"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEN5504001.1"
                     /translation="MNRIILLDRDGVINTDSINYIKNVDELIIIPDSITAIARLSAAG
                     YKIGVATNQSGVSRGLYSEDDLSAIHCKLREVLAEAGGQIDALEYCIHLPEDNCFCRK
                     PQPGMLKALAEKLNCSLDSVPFVGDRVSDIQAALAVGAQPIIVLSNMTDMSELAQYPK
                     VPIYNSLLQYVNQLLL"
     gene            complement(26120..28186)
                     /locus_tag="U6309_000547"
     CDS             complement(26120..28186)
                     /locus_tag="U6309_000547"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEN5504002.1"
                     /translation="MVNDFIFELGCEELPSGAVWPLANEFSTRLLAALDKAQLSYGEV
                     KRFATPRRLAIVIHDLQTEQESQKVMRRGPAAIAAYDSAGNPTPALLGFAKSCGVTVD
                     ALTKTQTDKGEWIVFETQSEGTKTKDLLPGLVSQSLESLPIAKPMRWGEGDDEFARPV
                     HWAVMLYGSETLNHKILGVTSGCHSRGHRFHHPQEIRINSAASYEDQMKDAFVIADFA
                     TRRQAIIHQVQELAAPLGASVVMPEELVDEVTSIVEWPQALIANFEQEFLEVPAEALI
                     ASMQSHQKCFALKNKHGELLPYFITVSNIASSNPKQVVLGNEKVMRARLSDAAFFFKQ
                     DKKQPLSAHIPSTEQVIFQVKLGSVYDKVQRIKVMMDHLSQSLNLPSHLAERATLLSK
                     CDLMTGMVGEFPELQGIMGYYYALNDGEEAVVATALNEQYMPRFSGDDLPSTDLGKAL
                     SLADRMDTLVGIFAIGQKPTGVKDPFKLRRHALAVVRLLISISAPLNLSTLIKEALAN
                     YGDRLPQDKNLVAELKPFILERLQSYYQNQGISADLVHAVRARQDDWLYDLDKRLFAL
                     KSFITMPEATSLSAACKRVGNILAQAAYADKKVDIKEELIEEGAEKALFDHLNKITKT
                     VETLYIAGDYQALLKLLASLKEPVDAFFDHVMVMVEEESLKQNRLALLQRLQELLQGV
                     ADISML"
     gene            complement(28176..29081)
                     /gene="glyQ"
                     /locus_tag="U6309_000548"
     CDS             complement(28176..29081)
                     /gene="glyQ"
                     /locus_tag="U6309_000548"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEN5504003.1"
                     /translation="MTKPTTFQEIILTLQKYWSDLGCVILQPLDLEVGAGTFHPATFL
                     RAIGPEPWSAAYVQPSRRPTDGRYGENPNRVQHYYQFQVVLKPSPLDIQDRYLDSLRA
                     LGVDPLLDDIRFVEDNWESPTLGAWGLGWEVWQNGMEVSQFTYFQQVGGLVCKPITGE
                     LTYGLERIAMFVLGVDNLFDLPWCKTANGIVTYGDVFHQNEVEMSAYNFEHANVEELF
                     KQFDYYEGEAQKLIALQLPLPAYEMVIKASHTFNLLDARKAISVTERQRYILRVRHLS
                     RAVAQAYYQAREALGFPMQKVCNGQ"
     gene            complement(29316..30101)
                     /locus_tag="U6309_000549"
     CDS             complement(29316..30101)
                     /locus_tag="U6309_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN5504004.1"
                     /translation="MKFTSLLTAGALASTLVSPAIMAADTASASLSDAQKKEIEKVIH
                     DYLINNPEVLLEASQALQQKQQQNMQQQAQAAIQENAEQVFQGKLTTVGNPKGNVTLV
                     EFFDYQCIHCKKMASTIENLVKKDSGLRVIYKEFPIFGKTSDLASRVALAAGMQGKYQ
                     AMHNALITIDKRLDEKTVMDAAKSIGLDMQKLKKDMDSQEVTDILDANRQLAEKLHLM
                     GTPAFIIGSTPDGQYKKGSEISFIPGATSEQSLRELIKKAAGN"
     gene            complement(30309..32474)
                     /gene="uvrD"
                     /locus_tag="U6309_000550"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(30309..32474)
                     /gene="uvrD"
                     /locus_tag="U6309_000550"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEN5504005.1"
                     /translation="MTIAALLKGLNEKQRDAVTSPLGNMLVLAGAGSGKTKVLVSRIA
                     WLIEEQHLSPHAILAVTFTNKAAGEMRSRLSSMLSTPTLGLWVGTFHGLCHRLLRRHY
                     KEANLPEQFHILDSEDQARVIKRVILSLNLDPEQWQVKQAQAFINSKKDEGLRPQHIN
                     ALHYGPTKTLVSIYKAYEDVCQTSGVIDFAELLLRTHELLRDNEEILAHYRERFQAIL
                     VDEFQDTNTIQYAWIRLLAGDHTAVLAVGDDDQSIYGWRGAKVENIQQFVHDFKDTQI
                     IRLEQNYRSTAMILNAANALIHNNSTRMGKELWTAGSEGEKILVYSAFNELDEARFVT
                     ERIGMELNQGASADEIAVLYRSNAQSRVLEEALLRVGIAYRIYGGVRFFDRAEIKDTL
                     AYLRLLVNPNDDTAFERVVNFPTRGIGEKTLDEVRQYARAEQCSLWDASKGILQSTGL
                     GQRGSLALAKFIDLIEKLQVVVANKELDEQISDVIQHSGLYAHFSKIKGDKSESRVDN
                     LQELINAAKQFRYEYDEEEELPLVNSFLAHASLESGELQADEHERSVHLMTLHAAKGL
                     EFPIVFLVGMEEGIFPGRQSIEEPGRLEEERRLCYVGMTRAMRKLVLSYAEVRRQYGR
                     EEYHRSSRFLREIPQQFLDEVRVKSRSQWPGTTKSKLPVADEVSGITIGQNVQHAKFG
                     QGVVLAIEGSGAHTRVQVKFSEHGVKWLVLAYANLTECL"
     gene            32695..33654
                     /gene="pip"
                     /locus_tag="U6309_000551"
     CDS             32695..33654
                     /gene="pip"
                     /locus_tag="U6309_000551"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEN5504006.1"
                     /translation="MHTLYPAIKPYTQHELKVSKLHTLYLEETGNPEGIPVIVLHHGP
                     GGEADPHLRRFFDPQRYRIILFDQRGCGRSIPHLEISENNTQLLMEDIDSIRDFLGLR
                     EFVLFGGGWGSLLALLYAQKFPQQVKALLLHQIFLGRQKDIDWFYKHGASLVYPDYWE
                     EFTNTVPENKLDNIPQYYAECLQGSNELARMAAAKNWALWQARCSSLQPHLYVIDQYS
                     DPHFALALATLESYYIINHYFIEENQVIANAHKIRHIPTYIVHGRFDLVSPLSSAWEL
                     HQAIPASNLRIVRDAGHSDRESGIIDALIYASKDISKQGLDAC"
     gene            33648..34085
                     /locus_tag="U6309_000552"
     CDS             33648..34085
                     /locus_tag="U6309_000552"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEN5504007.1"
                     /translation="MLTVLQRVKEARVDIDGQTVGKINHGLLILCGFEPKDSLENIKR
                     MLDKCINYRIFEDPSGKMNLSLKDVNGGLLLVPQFTLMADTQKGLRPSFSNAASPELG
                     RELFDNLLTLAQKCHQNTQSGCFGANMQVYLCNDGPVTFLLQF"
     gene            34302..35084
                     /locus_tag="U6309_000553"
     CDS             34302..35084
                     /locus_tag="U6309_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEN5504008.1"
                     /translation="MRLILMFASIMGSVILASCASKGSNPIDPFEPFNRKVHNFNMAF
                     DATVLRPPAKLYQAVVPKLVRKGVNNAFNNLDLFPTIASDILQAEGKWVIKDSWRLFI
                     NSTIGVAGLFDVAEKFGLPPHYNDLGLTLAKWGDKKSPYLVIPFLGPSTLRDGAGWLF
                     QFALWSPYVYIDDAGLVYGLIGLRYIDLRTQFFDSEPLLNEALDKYTFIRDAYLQHRN
                     YLIAGTEQDTGTSFVEDKKANKTAMQESTASNNQLGSDYVDE"
     gene            complement(35193..36152)
                     /gene="gshB"
                     /locus_tag="U6309_000554"
     CDS             complement(35193..36152)
                     /gene="gshB"
                     /locus_tag="U6309_000554"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEN5504009.1"
                     /translation="MKLAVLMDPLDQIKPYKDSTVAMIKSAQGLGWSCAYFTLNDLYC
                     CDGGAYARVHEIHIGDIESLSWVKTIDLGNKPLRDFDVILMRKDPPFDMEYIYATYAL
                     DLAEQEGVLVANKPQSLRDANEKFFTLNFPHCCPPTFVSRDARQLKEFWQIHQQVIFK
                     PLEGMGGNSVFYVDKEGKNLSVILEVLTKGQTKCIMAQRYIPDITVSGDKRILLINGD
                     PVPFALARIPAKGDLRGNLAAGARGEVVAITERDKWICEQIAPALREKGLYFVGIDVI
                     GDYLTEINVTSPTCIREISTETGLDIAGEYMRCLEKLHTSLSK"
     gene            complement(36152..37447)
                     /gene="gshA"
                     /locus_tag="U6309_000555"
     CDS             complement(36152..37447)
                     /gene="gshA"
                     /locus_tag="U6309_000555"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEN5504010.1"
                     /translation="MNKHEIPVPHLTTAHSGPLYHVEKIILNKLAEIESWFRKKWQET
                     PAPLTSSVDLRHAGFKLAPVDTNLFPAGFNNLNPEFLPLCIQAAQSALIEYIPDCTKI
                     LLLPESHTRNKYYLQSLNVLKTIFVKAGFIVRIGSLDPDIKEPTEIILEQGETLLIEP
                     LQRQGDKVGLDNFSPCLLLLNNDLSSGVPEILQDVQQRIRPTAKLGWASRLKSSHFQF
                     FEEVAIEFAELVGIDPWLINPYFSAIEGVDFMAQEGIETLAQEVDKILSLINDKYTTY
                     GIENKPFAVVKADNGTYGMSVMMVHNGDEIRQLNRKQRTRMSTSKGSRKVDRVIIQEG
                     VYTFETMPDGSVAEPVVYMIGQFVVGGFYRVHKGRGIDENLNAPGMHFEPLAFAQACN
                     MPCDDLEVVDCPNRFYVYGVIARLAALAAAREIAAIGGE"
BASE COUNT        11697 a         7738 c         6944 g        11152 t
ORIGIN      
        1 aatttacaca aacataacaa tgctatatat tccatcaatt tcaacataat acaaactact
       61 ttgaacaatt tatcggctat taagaaatca aaaatcacca tttggttggt aggagtattt
      121 tttttattat tccaattctt cttgcaacta tcctccggga tcattgtagg cgcaataatg
      181 cacgaagaac atatttcggc actcagcgca ggacttttaa gtagcgcctt ttactatgtc
      241 tatacttctt ttcaaattcc tgtcgggatt ctttttgatc gctacaatac ccgatatttg
      301 ttatcggtca atgcaactct ttgtgccatc ggatgttttg tatttgcctc gggacataac
      361 ctcccgactc ttttccttgg gcggttaatt attggcgctg gctcagcctt tgcctttatt
      421 ggtttaagcc atttgttacg ccaacatttt cccttaaaac aatacgcttt tatgataggc
      481 ctatcagaaa cgttgggatt tactgttaca gtattaggaa tgattggcat gggttctgtg
      541 atcagccatt ttggatggcg ttattttatt aacatagcag gaatcattgg attcctgatc
      601 gccagtttat gctggttgtg tatccccaat agcaagccag tcgtttcttt tccacatcaa
      661 tataaacaac aaattatccc cattctaaaa aataaattag cctggtttaa tggccttttt
      721 gtttgtctgg aattctcagt aattactgtt tttgccgcca tgtgggctgt tccttttttg
      781 caattaaaac tggattgcac tatcaaaaca gccagtatac taacttcaat ggtcttactc
      841 ggtgctggct taagctgtcc tttattgggt caaatttcgg tacatctcaa tagacgaaaa
      901 ccgctgattc atgcttcttg tctgtcaact gcgttattat taatacttgt tttatattta
      961 ccaactcaaa ataagctatt gataggtatt ttaatgtttt ctatggggtt aacctgtggt
     1021 gcttatatgc tagcctattc aatttctaat gaattggccc cacctgaatt cttgtccact
     1081 tgtactggct tcaccaatac cttggcaatg ctctcggctc ccttacttca accactcgtt
     1141 ggattttttt tagatgtact cagtaaccat aaaagccaat acagcttggc tgactaccaa
     1201 ctggcccttt tgattgttcc ttttgccatg ctattggcaa gcttatttac ttgcctcctg
     1261 ccagaaaaat caaattagac tatcccaaat tagtaaccgc ttgtactgct gactccttga
     1321 tttgcttaaa agtttgtttg gatataaaac aaaatccttt tggattttct tcagaaaagc
     1381 ctgcaactgt tagaggaaaa tcatcctctt gttttatggt attgactaat tttaacttca
     1441 acctgggcaa acccacgcta agtaaacatt gattatcggt tagggtttta atggaaacaa
     1501 attgagacat tttgacatta tttcctgaag ggtctcgcca acctgctacc gcagcccccg
     1561 ttgcatcagg ttccttgaga tttgcagaat aaaaaccatt atcattactt aatctgcaaa
     1621 aaatagtgct tatatcatga ggggaatttt gatccttgct aaaccataat ttgctgaacc
     1681 atgccttatt acctgttaca aactgttctt tattatctac tcccgtttgc acgtgcccat
     1741 ttaatcgact ggtctgtacc gcttgcaact ctaccccagg tcttaatacc tgtgtttcaa
     1801 catctttatt atcctgtttt aacatatcta ctttaaaatt aaatcctttt gcattttcga
     1861 ctttcacgcc aaaaatccaa ctgtcattga tcggattgta gcgcaaaaat gagcgcccaa
     1921 cttgccagtt caactgagtg gagctatcta ttttttcgtt ggcctcataa aacagaatta
     1981 actggttggt ttggccgtca attaatgcgg ttttagcata aaaatcagta ccttgccttt
     2041 gcatctgaaa aaaatagccg tatcgatcac cgctttcatt ggtgaccata cctgtaaata
     2101 cccaacttcc aacggttggt tcattcgttt gggctacttt gtcttcctct ggctcttcca
     2161 ctgcaaaaac actgccactt aaaaataaaa acatggataa tagcaataac caaattcgtt
     2221 ttggcatcat attagtaata accttgtaat tgttgatgtg accaaaatgc ttcccaaaag
     2281 gaatcattca actttgcttc aacgggtaaa taatataatt tatctgaact aaatgaataa
     2341 cattttacag catccttttc tgtcattata actggtagat cgatatcatt taaatcatga
     2401 ggtttaaatt gatgatgatc gggataagaa tatggattaa attttatacc taattgactt
     2461 aaagtagaat agaatcgttg cgggttacca attcctgcta cagctgctac ttcagaagta
     2521 aacaaatcat tactgacttc ttcctgagtt gacaatcgaa ctatgttttt aggaattaat
     2581 tccatagaat aagtaccttc tgcagcacct tgattaacaa taacaaaatc aacttgtttt
     2641 aatcgtgaat caggttccct taatggtcca gcgggtagac aaaaaccatt acccagtttt
     2701 cgcatcccat ctattactac aatttcaata gatcgcccca ttttataatg ttgcaagcca
     2761 tcatcactga taataatttc aaccgagtgc ttgtctaaaa ggtaccttac tgcttcattg
     2821 cgcttaggcg caataaccac tgggcaatta atttttcttg ccatcatgag cggctcatcg
     2881 cctactaatt cagctgaatc atttaatttt acttcgtatg gaaaatgttt tattgctgct
     2941 ttgtaaccac ggcttacaat ccctactttt aagccttttt gctgtatttt tttagcaatt
     3001 tcaatgacca gaggcgtctt gcctactcct cctacggtca cgtttcccac tacaataatt
     3061 ggtatagggt acagctgttg gcagaaacgc tgtaaatacc atcgtctggt tcgtataaca
     3121 attctgtata accaggaaaa tggaaccagt atccactgta aaaaatggtt accataccat
     3181 atcctgttca caaaaaatga cattaagcta ccacttcctc attaatcctg ccaaattgtt
     3241 gcactttata caactgagcg taatgaccat ccatatcaag taattcttga tgactacctt
     3301 gctcaacaat acgaccatgt tgcaatacga taatcttgtg agcatgctta atagtcgaca
     3361 atcggtgcgc aatgattaaa gtagttctac ctttcatcac ttgctccaaa gccgcctgaa
     3421 tataatgctc agactcacta tccagagctg aagtcgcctc atcgagtatt aaaattggag
     3481 catctttcaa aattgccctg gctatcgcta ttctttgcct ttgtccgcct gacaacaaca
     3541 ccccattttc tccgaccctg gtatcatagc catcaggtaa ttgcttaata aactcatcag
     3601 cataggccag tttagccgca gtaataattt gctctcggct cgcatcaaaa cgaccatagg
     3661 ctatgttgtt agccaaagta tcattaaaaa gtgtcacgtt ttgactgacc aaggacatct
     3721 gcttgcgtaa actttccaga ctaagctgtt gaatgggcat gccatctaaa gtaatcatac
     3781 cttgactcaa ttcataaaag cggggtaata aactggctat ggtcgtttta ccacttcctg
     3841 aatgcccaac cagagcaaca gacgttcctg cttcaattac aaaattgaca tcatgtaaaa
     3901 tattttgacc ctgtcggtat gcgtggtata catgcttaaa ctcaatttca ccacgtatct
     3961 tttcttttaa tatcaaacca ttgtttcttt ccaaaggcaa atcaagcaaa ttaaatacac
     4021 tttctgcacc agccaagcct ctttgtatgg ttgcgttaag agtagtcaga gttttcatag
     4081 gttttattaa ttgcaacatg gctgcgataa ttgccaaaaa agaaccggca ctgatagtaa
     4141 tgacagtaga taaatgaatg gcagccataa taatcgtggc aatcccgata gcgatcacca
     4201 attgaacgcc tgaaacatta atcgccttgc ttatggcaac tttcatatcg tttttgcgtg
     4261 aatattctgt tgctttatta aattttgtta tttcataccg ttcaccacca aagatgcgaa
     4321 ccactcgata accctcgatt gcctcactgg caatttctgt gacttcgccc atggtttttt
     4381 ggactttgtg actaattcgt cttactcttt tattagtata attaacaatg attcccacaa
     4441 aggggatagt taataaaaac atcaaagaca gctgccaaca tataaccatc ataacggtca
     4501 gcaaaccgat gaccagacat atattttgaa taaaatctgt tagggcatcc gcactgactt
     4561 gtgccacttg ctcaacatcg tacaaaattt ttgaaagaag ctgacctgat gtggcttcgt
     4621 cataataatc cgccggtaag tgaataatat gagaaaatac ggtctgcctt aatactttaa
     4681 ctactgagcg agccacccat gtcatacaat aactgcctaa agaactgacc aatcctctca
     4741 gagtaatacc gattaaaaca atcagcggga tttgtttaac aaaattcaga tcaatagtaa
     4801 taaagctttt atccaaaaaa agcttggtca tataggtaaa gccagcgtca atgcccgaat
     4861 ataggatatt cgccaatact cctaataata aaactggcca aaaaggtttt acatagctta
     4921 acaagcgttt gtatagcaga cgggatttaa tagggaggtt atttttcatt aatttagaag
     4981 ttaatcttgt acgcaaatgt tatgaattgt aactcttatt ctgtctaagc gccaatgttc
     5041 tatcatgaag tccacctatt ccatagttag ttttattctg gtgtaggcac aacgaaatcc
     5101 agccttttga acgttcaaat ctgaaactgt acctggttga gtagttacgg tggcgaattc
     5161 caaaccatac tgcttggcca gattcagacg agcaaacaat aattttttct gaaggccttt
     5221 ccctcgataa agaggtaatg tgctggtaac gcccaaatca caagcatcac catgaattgc
     5281 tattgtggcg ccagcaacca aattaccatg atcatatgct gcaaatgcta aaactccttt
     5341 tgcactggca tattggtaaa attgttcctg agcctcaaga aaaccaaatc caatagctac
     5401 tctttttgcc cactcttcca attcagactg cttcacttct cttataataa acccatcatc
     5461 ctcggagtga gcaaccagat ggtaactctt taaatcaaga actgaaacat tatttaactc
     5521 agttatgtag taacctcttt ggcttaaaaa attaattaaa tgatttccca caaaaggaca
     5581 taactcaata tcgacacgat tatggcctaa cgatttataa aaattttcta tagcttctat
     5641 ttcagcttta tattggttag gctctgtttt aaatccccag cctactacct gggatagaaa
     5701 tgactcaaaa ccagaaaaac aagctgctcc gccgttgatt tccagaattt ttccttgtga
     5761 gtaccgcttt gtcacctcaa tgtgactctg cttgatacac ccttccatgc gaacagccaa
     5821 ttccttagta atatacatcg atttcctcac caccaggatc agatagcatt taaacctgaa
     5881 acaatatcgt gaatcaactc cggtccctta taaactaaac cactgtatac ttgtaataac
     5941 gaagctcctg cattgatctt gtctttggcg ctctccaaac tgtcaatacc acccactcct
     6001 atcaatgtca catcattacc aacatattgt ttcaacaagc gcaagcagcg agtagataat
     6061 tccatcagtg gcctaccact taatccgccc tgttcttcag aacaaggcaa attttttacc
     6121 atttcccgag aacaggtggt attcgttgca ataataccct cgattccata ctgtaaaatg
     6181 atatctgtca tttgttttag tgtttcatct gtttcatccg gagatacttt caccactaaa
     6241 ggcacatggc gtccatattg atctgctaat tttatctgtt ctttttgtaa ctgggtgagt
     6301 aattcagcaa aataatctcc ttgttgtaac tggcgtaaat caggggtatt gggagaggaa
     6361 atattgatag tgacatagga agcatgatca tatactttac ggaagcaata aagatagtcc
     6421 tctgcagctt gatttaaatt agtctcttta ttttttccaa tatttatccc taatattcct
     6481 ttataatttg ctctttttac attttctacc agtacatcaa ctcccgaatt gttaaaaccc
     6541 atcctgttga taatcgcatt tgcctctgca atacgaaaaa gccttggttt gggattacct
     6601 gcttgtccct taggtgttac tgtccctaat tcaatgaatg aaaatcccaa tttggctaat
     6661 gcatccaaat gttcgccatt tttatctaaa ccagctgcca gtccaacttg atgagggaat
     6721 acaagaccca tagcatgaac aggttgaccg gctattttcc gaaaataaaa accagggagg
     6781 taatgtaata aggataatgt taatgaatgg gctttttctg catccaatct gaaaagtaaa
     6841 gggcgcagca gtgaatacat aaaattactc ctaaagcagg gagaatcatt aataaagaac
     6901 aattgaggta tgataacatt tttcgtgatg attttcgtac tgacgattaa taggaagcga
     6961 aataaaatta attcagataa ttttttatgc atctgcccag ataaatagcg aaatgacttg
     7021 ctcattatga ttgcaaatag tcctgttgca taaacgagct tattctgctg ataggctatt
     7081 ctccctgtct aaaagggaaa atcataggcg cgtctctatt aatcgttggt ccactcataa
     7141 taaagctaga atcttaaacc acaaaaactg tattcctctg cagtattaat atgttcttcc
     7201 ttaacaccag tatcactctt tgatgttttt ggacctcttg atttttgttc gtttaaatca
     7261 gaattaatca cttcttcatt agcactcaat ttatcttttt ttaatccatt tagaattttt
     7321 tgatgagaac cctgatcttt gcattgcctc tccatcgagc ttctgattat tggctcaaca
     7381 ggatgatctt gcgcctgatt atcagctata aaatatgcag ccagggtagc agcaaacact
     7441 cctgtagcaa agcctcccaa ggcaaacata agcgcagtaa ccgctggtaa cgcaaaaatc
     7501 ccggataatc caattgacaa tggaactaaa acgatggcta gagtcattcc tagcaaaaac
     7561 acaggaatta tagagagaga aactacaaaa tctgtagcaa gccaacccaa taaagcagtc
     7621 gctcctgaac tagcaaacga agtcaaaccc aagcttcccc aaaatttcat gaaaccaaat
     7681 ttttccccgg taacattatc ctcattctgt tgagataaag tgttttcacc ttccaaagag
     7741 ttggattgac actgtaaatc atcatgacta atcagcaact cagatttatc ttttgttaat
     7801 tcattgatca gaccatttcc attattgttt tgtggcaaac tgggattatt ttttaaccga
     7861 tgcgcaaacg ttgcagcaac cgctccaaga aacaatcctg atacaacaag tagtaaacca
     7921 ggtaaaataa ctggtgaagc caacacagcc ggagcggcgc tcataaggag cataaaccct
     7981 atataaaaac ccaaactgaa ggagtcaacg ataacagcag ctaatgtcat ccccgacgcc
     8041 aaccataaga taattgcagc tgctcccata cttaataacg cagcaatcat gtgagatctt
     8101 aaggaaggtg taaactcact gtttttctta tttgatactg ccattttttc ctccctgcat
     8161 accagtcact tactttaaaa aagtaatctc aattaaacaa cacattgttg cgcccaaact
     8221 cttagcattg cattacctga tttatgcaaa tgaaaagcag cctccctaac ctccactcca
     8281 tagttctcct gttgcaagcc tagtaaaacc ggtcctagaa attgtgaagt gttaccttct
     8341 ctatcaatca atggaaaaat tctcacttct ttggctaccc ttgctaattc acgaattgca
     8401 atcaggtgaa aatcaaccgt ttgatcgtca agatctgcaa aaaaataatg agaactcaga
     8461 gcaaaatcaa aggtaaaatc ggcaaatggt aaatgataat cggtaagccc caggtacctt
     8521 ccatcagcct taccttgttc ataatccgca aaaaactgct tcattccttc ttgtctttcc
     8581 tgaagcaatt tatccaaact accattacgg ctaaagtcaa attgatcctg atgctgccta
     8641 acctcttcag ccatattcgc aaaaatcatt tttgctttag caagcagcgt atctttatcc
     8701 aatacgaata aagggtcaca actcactact ccattttttt tcacttgagt ttgctggtaa
     8761 ttaacagcgc taggaccgca accgtattct aatagtcgtg aattctccac tccctggggt
     8821 aaatcaaaca tctcacgata gtcttcaatt ccatgccccc ataataccag ttttctcatt
     8881 agaaatactc ctcttctaaa tccatttgga ttacactttg gctaaagatt aggtgatttt
     8941 tttacccagg taaagaatca ttttaaccac tgccctgggt aaaggtgttt ttgagataaa
     9001 aatttaaaaa gtttggagag aaaagccacc ttgcgattga acaaatgaat atttaccatt
     9061 caaattcttt caatttttcg caataagaca tccgtatatt atcaaatgta tcagtgggtt
     9121 aaaaagattg tcttggaaat aaacaggaag gtgacaagta cgcttcattt gggtatagtc
     9181 tagcgtattt atcaacataa caggatgtca ctcatgtaca caggcttaga ctttcaactg
     9241 ggcgaaacgt atgacatgtt acgagacagc gtcaaccaat ttgcccaaac tgaaatagct
     9301 cctttagctg cacaaatcga tgagaaaaat acttttccta accatctctg gagaaaactg
     9361 ggagaaatgg gattacttgg catcacagta agtgaagaat atggtggtgc caatatggga
     9421 tacctcgcgc atgccatagc aatggaagaa atttccagag cctccgcatc agttggttta
     9481 agttatggcg cgcactctaa tttgtgtgtc aatcaaatct ttttaaacgg aaataatgag
     9541 caaaagcaaa aatatttgcc taaacttata agcggtgaat atattggtgc tttggcgatg
     9601 agtgagtcca actcagggtc cgatgtagtc agtatgcaat tacaagcccg ttctgctgga
     9661 gacaaatata ttcttgacgg cacaaaaatg tggataacca atggtcctga cgcggatgtt
     9721 ttggttgtct atgctaaaac agacaagcag gctggaagca aagggattac tgcctttatt
     9781 atcgaaaaag gaatgcccgg atttaaaaca gcacaaaaac tagacaagct tggtatgcgt
     9841 gggtcaaaca catgtgaatt ggtatttgat caatgtgaag tgccttctga aaatgttctg
     9901 ggaacagtaa atcaaggggt caaggtatta atgagcggct tggattatga acgcacaatc
     9961 cttgctgccg gaccagttgg aattatgcaa gcttgtatgg atgttgtatt accctatgtc
    10021 catgaacgca agcaatttaa tcaggcgata ggtgagtttc aatttattca aggcaaactt
    10081 gctgatatgt acactgattt gaatgcttgc agagcttatc tgtatgccgt tgccaaggct
    10141 tgtgacaacg gtagggttag tcgtaaggat gctgcaggag ttattttata tactgcagaa
    10201 aaagcaacac aaatggcttt acagtcaata cagattcttg gaggaaatgg ttatattaat
    10261 gaatatccta caggacgttt attaagggat gcgaagttgt atgaaattgg ggcaggcact
    10321 tctgaaataa gaagaatgct aattggtcgt gaacttttta aagaaacagc ataaggaatg
    10381 agacaatgga acataatgat gtcgtgatag tcgcagcaaa aagaacacca atgggagcta
    10441 tgctaggcaa tttatctgca ctttcctccc cagagttagg ggcagtagcg catatggcag
    10501 cgttgtctca atcaggtatt gctccggcag aaatcgatga agtgatcagt ggttgtgtat
    10561 tacaagctgg tattggtcaa gctcctgcca gacaggcagc aataaaagca ggcataccta
    10621 attcgacagg agccacgacc attaataaaa tgtgtggttc gggcatgaag gctgtgatgc
    10681 ttgcgcatga tttaattaag gcaggtacag ctaatgtaat tttggctagc ggtatggaaa
    10741 gtatgagcaa tgccccctat ttattatcca aagcacgagc aggatataga ctgggtcatg
    10801 gtgaactgaa agatcatatg ttccttgatg gattagaaga tgcctatgat aaaggacaat
    10861 taatggggtg tttcgctgaa acaactgcaa aacactttaa tttcagcaga gaacaacaag
    10921 acgaatatgc cttacgctca atgaccaaag cgctaaaagc aatagagaat ggtgcctttc
    10981 gagaagaaat cgctcctgtc accttaacta cccgcaaagg agatatcacg gttgacgtcg
    11041 atgaagggcc tgagacagcc aaacttgcca aaatacctca gttaaaacca gcctttcaag
    11101 cagatggtac agtcacagca gccaattcaa gttctatttc agatggcgcg gccagtttaa
    11161 ttttaatgag tgcctcaaac gcagaaaaac gtggcattaa gcccttggca aaaatcatag
    11221 cacatgccag ccattcccaa gccccacaat ggttcacaac cgctcctgtt gatgcaatcc
    11281 gtaaggtaat gaacaaagcg tcatggaaac aagatgatgt ggatcttttt gagattaatg
    11341 aagcgtttgc agtagtagcg atggcagcaa ttactcagct tgaattgaac ccggaacaag
    11401 tcaatataca tggcggggca tgcgctttag ggcatcccat tggtgcttcg ggagctcgca
    11461 ttctggttac tttaatgcat gcgctaaaac accagggtaa aaaacgcgga atagcttctt
    11521 tatgtatagg cggtggtgaa gcaacagcta tggcaattga gctaatttaa cggttaaaga
    11581 tgacctgggt aaaaccttta tttttaccca ggtttttata ttaaaactgg ttgctatttt
    11641 tatttgcgac cagataatgt tttacctatc aaaaccaact tggttataac aataataaag
    11701 gatgtacatg ctaagacata gtctcattta ccttctacta agtattctta ttgtcgtatt
    11761 tgcaaaatat gcccatctta tcgtagttta catcgatatg tttttcactt acgttaattt
    11821 aaaattaaca cctatattca gccaaacagg ttggggactg attatcagga aaattctagt
    11881 ccttatgtta ttacccataa tcatcgcagg aatcccagcg ctaatttacc gggcactcaa
    11941 aggaaaagac atgccccatt ttattgcgat tgtctggata atatggacca tcatagtctt
    12001 gagtgatatt ttgattcggt aaggataaaa aatggctaag ttaactactc aaatcaatac
    12061 cagcagtcag gaattcaaaa ataatcaggc gaatatgcaa gcattggtta ctgatttaag
    12121 agaaaaaata catcaaatct ctctgggtgg agatgaaaaa gcacgtacca aacatcaaca
    12181 acaaggcaaa ctacttccga gagaacgctt acaccagttg ctggatccag gtagtccttt
    12241 tcttgagcta tctcaacttg ctgcctacca agtctatgaa gacacaatac ccgctgcagg
    12301 aataatcact ggcattggaa gagtggcagg aaacgaatgt gtgattgtgg tcaacgatgc
    12361 cactgttaaa ggtggaacat attatcctct gaccgtaaaa aaacacttaa gggcacaaga
    12421 aattgcattg ataaaccatc ttccatgcat ctatctggtg gattcaggag gtgcctttct
    12481 accgcttcaa gatcaggtct ttgccgataa agaacatttt ggacgtgttt tttataatca
    12541 agcgcaaatg tcagcattaa atattcctca aatagccgtg gttatgggat cttgtacggc
    12601 gggaggagcc tatgtaccag ccatggctga tgaatctatt atggtcaaga atcaggcaac
    12661 catattcctt ggaggccctc ctctcgttaa agcagcaaca ggagaagtaa tcagtgcgga
    12721 agaattagga ggagccgaag ttcactgtcg ccactcagga gtatctgatc attatgcgga
    12781 aaatgacgct cacgcgcttc atttagcgcg agtcgccatt agcaatttaa atcgtaaaaa
    12841 accagattcc attcatagag tggatacagt tcctcctctt tatgatagcg aagatcttac
    12901 cggaataatt cccactgacc ctagaaagcc ttttgatata agagaaatta ttgctcgagt
    12961 ggtagatggt tcagagtttg atgaattcaa agcacttttt ggaacaaccc tggtttgcgg
    13021 ttttgctcgc ctatatggat atcctatagg gattattgct aataatggca tactttttag
    13081 tgaaagtgcg caaaagggca gccatttcat agagctgtgc tgtcaacgta aaataccact
    13141 tgtattcctg caaaatatta caggatttat ggtgggctcc aaatacgaag catcaggtat
    13201 tgcaaaacat ggagccaaaa tggtgacagc agttgccaat gctaatgtac ccaaatttac
    13261 catcattgtt ggtggaagtt ttggagcagg taattatgcc atgtgtggcc gggcatatgc
    13321 tcctcgtttc ctatgggcct ggcctaatgc acgcatctca gttatgggtg gtgaacaagc
    13381 agcgaatgtt ctcgcgcaaa tcaccagaga gaaatatgca aagcaaggaa aagaatggtc
    13441 actcgaagaa gaagaacaat ttaaaactca aatgagaagt cagtatgaaa ctcagggaaa
    13501 tccttactat gccagtgcca ggctttggga tgatggggtc atcgcgcctc aggatacacg
    13561 caaaatactc ggacttggat tatctgcagc cttaaacgca ccgattgagg atactcgttt
    13621 cggtgtattt cgcatgtaaa aggaaaggaa caataggcat gagtgattta ttatatgaaa
    13681 ttcaagataa agtaggttta cttactatga atcgaattag caagcataat gcgttcgata
    13741 atcaactgct gactgaaatg agaatacgac ttgactccgc aataaatgat actaacgttc
    13801 gcgtcatagt acttaaagcg aatggaaagc atttttcagc tggtgctgat ttgacctgga
    13861 tgcaaagcat ggctaacttt actgaagaag aaaacctgga agacagtctg gtattaggta
    13921 atttaatgta tagcctcagt caaagcccaa agcctactat cgccatggtt caaggttccg
    13981 cttttggtgg tggagctgga ttggcagcag catgtgatat agccattgca tctacttcag
    14041 cacgtttttg tttttctgaa gtgaaattag gtcttattcc agcagtaatt agtccctatg
    14101 tagttagagc aataggtgaa cgagcggcaa aaatgttatt catgagtgct gaagtttttg
    14161 atgccacacg tgcatattct cttaatttag ttcagcactg cgtccctgat gatacgttgt
    14221 tggagtttac tctcaaatac gcctcgcaaa tcagtaataa tgctccagaa gcggttaaaa
    14281 actcaaaaca actggcacaa tacgtcgcta ataaaaaaat agatgaagaa ctggttcgct
    14341 acactgcttc tctgattgct cataaaagag tatctgatga ggggcaagaa gggcttaaag
    14401 catttctcaa taaagaaata cccaactgga ataaaggttc tggacatgtt taataaaata
    14461 ttaattgcta accgaggcga aatagcctgt cgaatcatta aaacagcaca ttctatgggt
    14521 attcaggcta ttgccgttta ttcagcagca gatagaaaca gccttcatgt gcgattagct
    14581 gatagcgctt attatattgg agaagcgcca gctaaagaaa gttatctgaa tattgaccat
    14641 attattcagg cagctaaaga aagtggagca caagccatcc atcctggcta tggattttta
    14701 tctgaaaatc ctgattttgc caaagcctgt gaacaagcag gtattgtttt catcggtccc
    14761 tccattaaag cgatggaagc gatggcatcc aaacaattag ctaaacaact tcttgaaaaa
    14821 acgaaagtcc cacttacacc gggctatcat ggcgttgaac agtctgaaga aaaattgttg
    14881 agcgaagcta aaaaaattgg atttccagta ctgattaaag ccgctaatgg tggtggtgga
    14941 aaaggaatgc gagcagtaca tgatgaaaaa gagtttcatg atgcattagc aggagccaaa
    15001 agagaatcta tggcctcctt tgcggatgat actatgataa ttgaacgatt agttcttaat
    15061 cctcgtcatg ttgaagtaca gatcatggcg gacaatcatg gaaatgttgt acatttgttt
    15121 gaaagagatt gttcaattca gcgcaggcat caaaaaatta tcgaggaggc ccctgctccc
    15181 aatttatcgc ccatgcttcg gcaaaggctg gcagaagcag cctgtgaagt agcccgctcc
    15241 atcaactaca gaggagcagg aacagtagag tttttggtag acggtgaaga taaattctac
    15301 tttatggaaa tgaatacaag acttcaagta gaacatcctg tcacagaaat gattacagga
    15361 ctcgatcttg tcgcttggca gataagaatt gcagcgaatg acacattacc tttgcttcaa
    15421 aatcaaattc aagctcaagg ccatgcaatc gagtgccgta tttacgccga agatccttat
    15481 cagggtttta taccctctat tggtcaactt caattcttaa aagaaccctc tggcgatggc
    15541 atacgcattg atacaggtgt tactttgtct tcagaaataa caaggtatta tgatcccatg
    15601 atagccaagc tgattgcctg gggacataat cgtgaagaag cattacaaag gttggagcgt
    15661 agcctgtctc actatgacat tggcggggta aaaacaaata taccgtttct acgagccatt
    15721 tgccgacatg taaaatttaa agaggcaaaa ttaagtactg attttttaga gaaagaaaac
    15781 ataaatctgc ctaaaccaga taatgagtta ggtatactcc tggccataag ttgtgattat
    15841 ttaggtatgg ttaatcgcac tacagatccc ttgttacaag atgcatttgg ctggcaaatg
    15901 catctttcca gccattgggt atggcgttat caattgaact ccacgataat tgaagctcag
    15961 attacaccta tagatagtaa aaaatttaaa gcaaaaattg aaaacaagga aatggtcatc
    16021 catgccagat atgaccttga ccagcttatt attgaaattg atcaaaaatc ggtgaaagcc
    16081 agagtagaaa acaaagaaca tcatttgatc ttctatactg acaaaggcca actttcaatc
    16141 gaacgcttct actggagcaa actggatgct caaacttctg cccataaagg ccaattaacc
    16201 gcgccaatgc ctgccactgt cgttgctatt ttaaaaaata ttggtgaaca ggtaaaagcc
    16261 ggtgaaagct taattgtact tgaggcaatg aaaatggaac acaccattca tgctccaatc
    16321 gatggtatat tatcagatat tttttattct gtaggttccc aagtgagtga gggagcagaa
    16381 ctgttagcac ttagcgaatc ggatacttaa gagaatattt atggactatc ctcagcatgt
    16441 gacaataatc gaagtaggtc ctagagatgg cttacaaaat gagccatctt ttctccccag
    16501 tgataaaaaa attgaattaa tcaatttact cagtcagaca gggctgaaag agatagaagt
    16561 taccagtttt gtttctgcaa aagcaattcc acagctagct gacaatgaag aagtatttca
    16621 atctattaat aagactccct ccatcaacta ctctgccctg gttccaaacg aacgtggaat
    16681 gcttaaagct ctggaaatgg gagtacaaaa tattgcagta ttcactgcag caagtgaatt
    16741 attcaatcaa cgcaacatta attgttctat taaggaaagt atagaacgct ttaaacctgt
    16801 tttagcatta gcaaaaacta atcaaattag agtgagaggc tatatctcct gcgtattggg
    16861 ctgcccctat gagggatata tacaaccgag tcaggttgtt agtgtcacta aaatgcttct
    16921 tgatctgggc gtacacgaaa tttcgttggg agatactatt ggcgtgggaa ctcccaggca
    16981 aactcagttg cttcttgatg ccatactacc catacttcca atcacacaat tagccatgca
    17041 tttccacgat acttatggac aagccgtagc aaatatttat gcctcccttg agtatggagt
    17101 taaccgcttt gacagctctg ttgcaggtct tggaggttgt ccatatgctc gaggtgccag
    17161 cggcaatgtt gcaacagaag atgtacttta tctgatgcat ggattgggta ttgatacagg
    17221 tgtagatata ttcaaaattg ttgctgccgg agatatgatt tgcaaagcat tagggcgaaa
    17281 aaatcaatcc aaagtcgcta atgccatgct agccaatcca tgtaattagg aagtctcatc
    17341 attattggct ttgataagta ctaaaaatca gaaacttaaa gagaagtgcc cggttttgtg
    17401 ctagcctata cttataaaca aattactgat gaaattttcc agtgtaacac ctatcattca
    17461 aaacagaact atgagagaaa ttttcatgaa cgacaaagta tggacaccta aaaaccctga
    17521 acaaacaaga atgtggcaat ttatggattt tgccgcaaaa aggcattgtc aggtttttga
    17581 aaattatcaa gacttacata cctggtcagt aaagcatcct gaatcattct gggaaactct
    17641 ttgccttttc tttaaattga actttgatac accaccgcac caaattctga attattattc
    17701 tgagatgatt gaagcccgct ggttttcagg agctcgcttc aatttcgctg aaaaattatt
    17761 aacccgtaga gataatcacc ctgccctaat aagccttgat gaaaatgact ctaaaagtgt
    17821 cattagctat gctgaattat atgaaaaagt agcccaatgc gctgcaggtc ttaaagcagt
    17881 tggtgtcgcc actggcgacc gggtggcggc agtgatgcct aatacttctt acacaatcat
    17941 tgccatgctg gcagcaactt ctttaggggc aatctggtca tcctgctcac ctgactttgg
    18001 tacccaggct gcacttgaca ggataggaca aattgaacct aaagtactct ttatttgtga
    18061 tggtcatcaa tatcaaggta agaaacacag cagctctgaa aaggtcgtac aacttactgc
    18121 tgcgattact tctctaagac aggtagtaat ttgcccgaat attaatgagg aaattaatct
    18181 cgaacaactg cctaacgcgt cgtattggaa tgattttata aaacctgaaa aagaatgtga
    18241 tttcatttct ctgccttttg atcatccagt ttatattttg ttttcttcgg gaacaacggg
    18301 taaacctaaa tgtatcgttc atggcgcggg aggtacttta attcagcatc tgaaagaatt
    18361 aggtcttcat tcagatatta aatccacaga caatttatgt ttttatacga cgtgtggctg
    18421 gatgatgtgg aactggactg tttctgcgtt ggcattagga gctaccatca ctctatatga
    18481 aggttctcca acctacccca cagataatag aatgtttcga ctgatagatg aagaaaaagt
    18541 cactgtattt ggaactagcg ccaagttcat atccgccatt gaaaaagccg gggtaaaacc
    18601 aaaaagtgaa tttcaattat cggagttgcg ctgcatcttg tctaccggct cccctcttct
    18661 gcctaaaaac tatgatttcg tctatgaaca tattaaaaaa gatctgcaat taagttctat
    18721 ttcaggagga acagatatta tttcttgttt tgcgttggga aatcctattc tcccggttta
    18781 tcggggtgaa ttacaatgca ttggattagg tatggaagtt gcggtgtatg atgagcaagg
    18841 ccattcaatc aaacaaaaac gtggtgagtt ggtttgcacc aaacccttcc cttccatgcc
    18901 agtctgtttt tggaatgata aggatagaca agcatataaa catgcttatt ttgagcgctt
    18961 cccaggggtg tgggcacatg gcgatttcgc tgaaataact gcccacaatg gattaattat
    19021 ccatggacgt tccgacgcta tattaaatcc aggaggagtg cgtataggaa cagctgaaat
    19081 ttacaggcaa gttgaaaaag tagatgatgt tgttgatagc atagtcattg ggcaagattg
    19141 gcaggatgat gtacgagtgg ttttattcgt taagttgcgt gaggggaaaa aactggacga
    19201 tgaattaatc aatctcatta aaacaaccat tagaaaaaat gcctcaccac ggcatgtgcc
    19261 agctaaaata ctacaagtcc cagacattcc taaaaccctt agcgggaaag ttgtggagct
    19321 cgctgtcagg caagttgttc atggtcaatc agtaagtaac ttacagtctt tggcaaaccc
    19381 acaagcatta gaatatttta aagacaggga cgaattgaaa ggctaaaatt gcgtgaaaat
    19441 agctatttcc agcacgaatt cacattaaaa ttcatctcgt atcaaccaat agaaagggca
    19501 attgcaagag ttgccctttc atgtcaatac ttattattta aatattattt gaatgaaaaa
    19561 aatttaataa tcctcataat ttgaggaaaa ctcatttacc cgaatacttt aaaaccatcc
    19621 cctaatttac aattcatttg ataacttcct aatcgctcat aactacttgc attcagcaca
    19681 gtggttttaa ttgaagcatc accctcagaa tctaaattaa acttactgta atcaaacata
    19741 ggtgtaccgc gagccaccgg atcgtcaaga gtaaaatgcc tatctgaggc tgtaacacgg
    19801 ctatcaccga ctaccataac tgcattcgga gtaactgaaa ctattgcaga attcaatggc
    19861 atttccgaag tacatttttt taagttaatt acaaaagtga ttcgctttcc ttcagctatg
    19921 gcatctacta tattttgaaa agtttttaat tctactgctg cctgcgctgt gaatgagcac
    19981 agggtaaaaa ctgctaaaaa catctttttc atgaataata ttccttgtat taaaaccggc
    20041 ttaaggttgc caataagaat ttatcttgtc aatcctcaac caaaataatt attttcagac
    20101 cgaatgacac cgcgtatctg attaatgcga attatctctt tttatataaa ccaaaagcca
    20161 accaataatc atgaaaaaca aattgagata cctcaacaga attaagattt ttctttgttt
    20221 atgaatccag tttgtgtata cttgtgcata gcgtcgattt gaaacacagc ttgcggacgc
    20281 tcaaaagatt tattcctctg tttaagaaca tgaaaactct aaaatcgagg taaaatgcaa
    20341 ttattgcttt ttattaagaa ttattgtttt ccagttcttc agatatcttt tgaaaatacg
    20401 gctaaagcgg ttgctttcta atccccactt tatgccccgc aagccatatt tccaggttat
    20461 agtaatgatt gaattatgtg ggctaaaaaa atctttctct ggtaaactcg ccttaaacga
    20521 tattaattta tttattcagg aaggtgaaat ttttggagtt atagggaaaa gcggggcagg
    20581 aaaatcaaca ttactgcgtt gcatcaatat attggaaaaa cctgacgaag gtgaagtcat
    20641 tattgatggg cagaatctga tgagcctttc cagaaaagat ttagccttgg cacgtcataa
    20701 aattgccatg atcttccaac actttaattt gctaaattca aaaactgttt ttgataacat
    20761 agcccttccg atgagaattc aaggtataga cgaagagtcg atcaaacaga aaattgaaga
    20821 gctgcttcct gttgtcgagt taacagataa gaaagatgct ttcccctcac aacttagtgg
    20881 tgggcaaaaa caaagagtag caattgcgcg agcactaagt tgttctccca aagttctatt
    20941 atgtgatgaa gccacttcag ccctcgatcc cgcaactact gacgccatat tatcactact
    21001 aaaaaaaatc aatgaattgt atggaattac tattgtcttg attactcatg aaatggatgt
    21061 cgtaaaacgt atatgccaaa gactgtctgt catggtcgat ggcaagattg ttgaaactac
    21121 tgctttatct aatattttca ataagccaga gagtctcgct cgcaaaatgc tttacgcaca
    21181 aattagccct gaactcccaa cctgccttac tcgcaggttg gcggactatg ctactgaaaa
    21241 acctctcgtg agattatttt ttcagggcga ggaagcaacg gtacctttta ttagccaaac
    21301 cagcagagaa ttaaacatgg acatcaatat tctactggca aatattgatc gtttcgatgg
    21361 agtgacttgt ggtgttttgg ttgttgagtt aacagcgaat cctttattgc ttgaagcatt
    21421 tatcaatcgt tgcgaacaag ctggtattac tgtggaggtt ttaggctatg tcctaccaga
    21481 tggtttatga tttacttagc gcaacaggag aaacacttta tatggtgttt tttagtactc
    21541 tatttgcagt cctccttggc ttgcccttag gtatattgct gtactcatca tctcggataa
    21601 aaccaaacat taaattgaat aaaattttat ctgctctgat taatattttc cgttccatcc
    21661 cgtttatcat tttacttgtc gctattattc catttaccag gctaattgtt ggtaccagca
    21721 tagggatcaa tgcagcaatc gtcccattaa cagttggtgc gactcctttt tttgcaaggc
    21781 tagttgataa tgttctccaa tcgctacccc ttggtcttat agaaaccggg tattcaatgg
    21841 gagctaacac acgccaaatt attttacata tcatattacc tgaggcacaa tcaggattaa
    21901 ttcattccat tacagtaaca gcaatcactc tgattaatta ttcagccatg gcaggagctg
    21961 tgggtgccgg aggattaggt acattagcga ttaactatgg ttatcaacgc tttaatgctg
    22021 gcattatgat agctactgtg attgtattaa ttattttggt ccaactgata caaatggttg
    22081 gagactactt agccaaacgt tgtacacatt attaattggg aggttcaatg agaattttaa
    22141 gttgtttggt tttaatcatt agcctggtag cttgcagtag taagccagct cccaatactt
    22201 tagtcatcgg cacaattgct ggccctgaaa cagaacttat tgaaacagca aaacaagttg
    22261 ctgaaaaaga gtatgggctc aacataaaaa ttgttgagtt taatgattat aatttgccca
    22321 atgaagcact gcaagatggc agtctggatg ctaatgttta ccaacattta ccttatttaa
    22381 aagccgctat actgtcccat ggttatgatt tgcaggcaat cggcagaacc ttcgtttacc
    22441 caatgggtat ttattcgaag aaatataaaa cactcagtga attacctgaa aatggaataa
    22501 ttgcagtccc taatgaccct agtaatgaga tgcgtgcctt cttattattg gagaaagctc
    22561 atttgattac tcttaaaaat acaacgaata gcggtattca agatatcgaa agtaacccca
    22621 aacaattcaa attcaaagag atagacgccg ctcaattacc cagagtactc cctgatgttg
    22681 acgccgcagt aattaacacg acgtttgcct tgcctgcggg attaagccca tcaaaagatg
    22741 ctttgtttac tgaaggcaag gattcacctt atgccaatat cattgtgata cgccgtgaca
    22801 ctgaaaaaag gccacagctg gaattgtttg tgaaggcatt gaattcagaa gaagtaaaag
    22861 agaaagcaaa aaaactgttt ggagaagatg caataccagc ctggtaatta ttaataaatt
    22921 agcttaattt ttacttaata aaaaactgtt atactttatc tagataacct tattgaggta
    22981 tttgatatgg ctttttataa ttctatccct ttaattggta aacatttaaa atcagagagc
    23041 cgtgcaaaaa aatacacttc tgaagaattt aaaaggctag agagaaaata cagagatcac
    23101 atcggtttgc cagaaaactc aagctttagt tctgacgcat tcgataagct tgtcagctcc
    23161 agagtggatg atttccgggc ttttccaaaa accagcctgc ccggggggtt gctgtttcat
    23221 gataaactga aggaagccaa tactaccgaa ggggaaaaca tccgggatgt cttaagtaaa
    23281 acagtcctgg caccaactga aaaatttaaa acagcgcaaa aaaattttac agaatcaact
    23341 tcaacattta aagagctcac caagttaatt ccaagtaaat tccgagccca ggatcttgtt
    23401 ggcaccatga aggaactgac tgatgatgct cgtaaagcga taatagaaca gcaagcgcat
    23461 gaaatccaaa tgttgaaaga aaaattggac cctcagaaag aaggacaagc tggtaattat
    23521 gtatccgatt ttaaaaaagc tctgggtctt aaggataatg acgaagttaa gaaagttaca
    23581 gaaggcttga ttaaggaatt ggaagaaacc cataaaaaac aactgggtga attcgataaa
    23641 tccacaagcg actcgttaaa ccaactacat aaagcctctg cgagtgaatc atcagagtat
    23701 ctgtttcttg ccaatctgta tgaaaacaat aaggaaatgc gtcaaacgct tgaaaagtta
    23761 tatgccaaag aacgccaaag aaaagggctt cctcctgaat ctacaacagt tcaagtaggt
    23821 atatcagaaa acaaaattga tctcaatgga atcaaactca aagatattcc tgtcattaaa
    23881 agtattacag ggagagagat tcatcaacaa ccggatggca gttttacaat ccagatgccg
    23941 atgataaatc cacgttacta tcaggatcca agacaaaatg tcaaaacaga catgatgctc
    24001 attgctcaag ctataaaagc ctcaggttat gactctattg aaatgaattg caattttcca
    24061 aatagtgaaa aggtagcgat ggaaaggggc aggcaagctt ttgccgcctg tattgaagca
    24121 ggctttcctg ttgacaagat caccattaat gttaacggca aacctatgaa ggcggacgaa
    24181 ttgtttaaag aacatccgaa aacatatcag gatattatgt caagagcctc aaaaatctcg
    24241 aaagattacg aggacatcaa agcgcccaaa acatcaactg ctccagtaaa caccgtcgcc
    24301 gtcaaacagg aaatcgccac attacgcgcc aaacagcagc aagaagaacg agctcaaaca
    24361 caacctgttg atgaacaacg acatggcatg acccccaata attgacttat aagttgaatt
    24421 tggattgttt aatccaaatt caacaaattc attatcctcc ccgaattttg ctcaaaaaat
    24481 ttcactacct caaaccatca atttctgata ttttatccat tctagacact tgaattttaa
    24541 atgacagatt ctttgtaccg ccacttatta gaagtatcca taaagaggtg gcatttaaaa
    24601 ataaagtgcc agtataacta ttccaggtat tttggtatca tcgagaatag atatggaatt
    24661 caatgggtta gctaaggaga aagctatgag tactcgtcgc tttgatgaat ttgacaatga
    24721 aatggatgat tttagtgatg ctgaacctgt tataactgag gcaagccctt caacaaaatt
    24781 ggaaagacgt agaaaaatag aagatctgtt ggaggaaaag cggctaagag aagaactgga
    24841 agagtttggt tagttggaag aaaattgagg catataaatg tcaaacctta cagtcttacc
    24901 atcaattgag gcatctggcg ggagtaatgg ttttaccttt tgcggccact tgactacaac
    24961 tcttgactta acatgagata aagacaactt gattaactcc attgcatcat aatccgtacc
    25021 aattagttgc tgcaaaactt gcatctcttt cttaaccaaa gccgatttgt ttcgttctgg
    25081 gtgcatggga tcaatgtaaa taatatcggg ataatccttt tcttgcaaac tatgcaggaa
    25141 tgaaatagcg tcacttgcga tcaaagacaa acacattttt tgaatgtctg cttcgtttct
    25201 acgagacagt gcatcagcaa gcagtgcagc catgaccggg tggcgttcca gcataagcac
    25261 atcggcacca aaggttgcca aaatagcggc atcttttccc caacctgctg tagcgtccaa
    25321 aattttaatg ccttttgttg gcttgcaagc cctgatcaag ccctgttttt taccctcccc
    25381 tcttctttta ctccaggtca tagtactgaa atcagcaaac ataagagaaa aatttcgaat
    25441 ttttaaagtt aatttttcag gagtaacaaa aaggcaggta tcagcatttt gatctaactg
    25501 taaattcaat tgatcagcta acaattgtgc tttttcttta agctcattat tttcataacc
    25561 aactgctttt attctattca ttcttacaat aataattgat tgacatactg taacagggaa
    25621 ttataaattg gtactttagg atattgtgcc aactcagaca tatcagtcat attagaaagt
    25681 acaataatag gctgggcacc aactgccaat gctgcttgta tatctgaaac cctatccccg
    25741 acaaagggca cgctatccag tgaacaattt aacttttctg ccagagcctt tagcattcct
    25801 ggctggggtt ttcggcaaaa acagttgtct tcaggtaaat ggatacaata ctctaaggcg
    25861 tcaatttgcc cccctgcttc tgcaagcacc tctctcaact tacaatgaat ggcagataaa
    25921 tcatcctcag agtatagccc tcttgaaact ccagattgat tggttgcaac accgatttta
    25981 tatccggcag cacttaagcg agctatcgct gtaatgctat cgggtataat aataagctca
    26041 tcaacattct tgatataatt gattgagtca gtgttaatta ctccatctct atcaagcaat
    26101 ataattcgat tcataagtgc tacaacattg aaatgtcagc tacaccctgc aataactctt
    26161 gtaatcgctg caggagggca agcctgttct gtttcaatga ctcctcttca accataacca
    26221 tcacatgatc aaaaaaagcg tctactggct ctttaagact agccagaagc ttgagtaatg
    26281 cttggtaatc tccggcaatg tataaagttt ctactgtttt agtaatttta ttaagatgat
    26341 caaacaatgc tttttcagcg ccttcctcta taagctcttc cttaatatct acttttttat
    26401 ccgcatatgc tgcctgagcc aagatattgc caacacgctt acaggcagct gataatgatg
    26461 tcgcctctgg catagtaata aatgacttta aagcgaatag acgcttatct aaatcatata
    26521 accaatcatc ctgacgagca cggacagcat gaaccaaatc agcgctgata ccttgatttt
    26581 gataataaga ttgaagcctc tcaagaataa atggttttaa ttcagccact aaattcttgt
    26641 cttgagggag tcgatcacca taattagcca aggcttcttt aatcaaagtt gataaattaa
    26701 gcggtgctga tattgaaatg agtaaacgga ctacagccaa agcgtggcgt cttaatttaa
    26761 aaggatcttt tactccagtt ggcttttggc caatcgcaaa aatacccacc agagtatcca
    26821 tacgatccgc cagggaaagt gctttaccca aatcagtcga tggtaaatca tcaccagaaa
    26881 atctaggcat gtattgctca tttaaagcgg tagcaacaac agcttcttcg ccatcattaa
    26941 gagcataata atatcccata atcccttgca attcaggaaa ttcgcctacc atacctgtca
    27001 tcaaatcaca cttgcttaat aatgtagctc gctcagccag gtgagagggt aaattgagag
    27061 actggcttaa atgatccatc attaccttta ttcgttgtac cttatcatac acgcttccca
    27121 gctttacttg aaaaataacc tgctcggtag aaggtatatg agcgcttagc ggctgttttt
    27181 tatcttgttt aaagaaaaag gcagcatcac ttaatcttgc tctcattacc ttctcatttc
    27241 ctaaaaccac ttgttttgga ttggagctgg cgatatttga taccgtaata aaataaggta
    27301 gcagctcacc atgcttattt tttagtgcaa aacacttctg atgagattgc atggaagcaa
    27361 taagggcttc agcaggaacc tctaaaaatt cctgttcaaa atttgcaatg agcgcttgag
    27421 gccattcgac tattgaggtg acttcatcaa ccaattcttc aggcataacc actgaagcac
    27481 ctaaaggtgc cgccaattct tgtacctgat ggataatggc ttgtcgccta gtagcaaaat
    27541 cggcaattac aaaagcatct ttcatctgat cttcatagct tgcagctgaa tttattctta
    27601 tttcctgcgg atgatgaaaa cgatgtcccc tgctatgaca tccacttgta acgcctaaaa
    27661 ttttatgatt aagtgtctca cttccataaa gcatcacggc ccaatggaca ggtcgggcaa
    27721 attcgtcgtc tccttctccc catctcatgg gttttgcgat aggtaaagac tctaaagact
    27781 gactaaccaa tccaggcaag agatcttttg ttttagtacc ctcactttga gtttcaaaaa
    27841 caatccattc acctttgtca gtttgagtct tggtgagcgc atctaccgta acaccacatg
    27901 attttgcaaa tcctaacaaa gcaggagtcg ggttacctgc actatcgtat gctgctattg
    27961 cagcaggacc gcgtcgcatg actttttggc tttcctgttc agtttgtaag tcatgaatca
    28021 caatagccag acgtcttggt gtagcgaatc tcttgacttc accataactt aattgcgcct
    28081 tatctaatgc ggctaacaat cgagtagaaa actcatttgc caaaggccaa acagcaccag
    28141 aaggcagctc ttcacatcct aattcaaaaa taaaatcatt gaccattaca caccttttgc
    28201 atagggaaac ctaacgcctc gcgcgcttga tagtaagctt gagctactgc tcttgataga
    28261 tgtcttacac gtaatatata cctttgccgc tctgtcacag aaattgcttt acgtgcatcc
    28321 agtaagttaa aagtgtgaga agcttttatt accatttcgt aagcaggaag aggtaattgc
    28381 aaagctatca atttttgtgc ctctccttca taataatcaa attgcttaaa taattcttca
    28441 acattggcat gttcaaaatt ataagctgac atctctactt cattttgatg gaatacatcc
    28501 ccatatgtca ctataccatt agctgtttta caccaaggca aatcaaataa gttatctacc
    28561 cctaaaacaa acattgcgat acgttccaaa ccataggtta attcaccagt gattggttta
    28621 catacaagtc cacctacttg ttgaaaataa gtaaactgag acacctccat tccattttgc
    28681 caaacctccc aacctaagcc ccatgcgcct agagttggtg actcccaatt gtcttcgaca
    28741 aaacggatat catccaataa gggatctact ccaagtgcgc gtaacgaatc caagtatctg
    28801 tcttgtatat ccaatggcga cggttttaat accacctgaa attgataata atgctgaacc
    28861 cggtttggat tttctccata acggccatcc gtcggccttc tggatggctg aacataagct
    28921 gctgaccatg gctcaggtcc aatcgctctt aaaaaagtag ctggatgaaa ggtacccgcg
    28981 ccaacttcca gatccaaggg ttgcaaaata acacaaccaa gatctgacca atatttttgc
    29041 aaggtcagta taatttcctg aaaagtagta ggtttagtca tgaaaatctc aaaaaacaaa
    29101 tatgatcttt aaatataggt tggtttttaa tccttttatc caggatatat cccataaaac
    29161 ctatctttca tttagaggat taattattca gtccaataaa ttattggatt gttgggtgcc
    29221 agggttgatt gaactcaacg ccacaacact aaaatactat cccagttgag gacaactggg
    29281 atatatgaat ttaatccaag tattcagata aatacttaat tgccagccgc ctttttaatt
    29341 aactcacgta gtgattgctc gcttgtagcg cctggtataa atgatatttc actgcccttt
    29401 ttatattgac catctggtgt tgagccaatt ataaaggctg gtgtacccat caaatgtaat
    29461 ttttcagcca gttggcgatt cgcatccagg atatcggtca cttcctgact atccatatct
    29521 tttttcagtt tttgcatatc cagacctatg gatttggcgg catccatcac tgttttttca
    29581 tccaaacgtt tgtctatcgt aatcaaagca ttatgcatag cctgatattt gccttgcatg
    29641 ccagccgcca gagcaactct ggaagccaga tcagaagtct tgccaaagat tggaaattct
    29701 ttgtatataa ctcgtaatcc gctgtctttt ttaaccagat tttcaatggt agatgccatt
    29761 tttttgcaat gaatgcattg ataatcaaaa aactcaacca gtgttacatt ccctttagga
    29821 ttacctacag tagttaattt gccttgaaaa acctgttcag cattttcctg aattgcggct
    29881 tgagcttgct gttgcatatt ttgctgctgt ttttgttgta atgcttgcga agcctccaat
    29941 aaaacttccg ggttattaat cagataatca tgaatgactt tttcaatttc ctttttttgg
    30001 gcatctgata aagaagcgct agccgtatca gccgccatta ttgcaggtga tactaatgta
    30061 gaggctaatg ctccagctgt taataaactt gtaaatttca cgtaattccc cttacttata
    30121 attaatcaat gacgcaaatt acatctcgca gataacttta tcattaattg atctgctcaa
    30181 aacagcaaag tataacaaat ttctgttgcc taacgcaccc tagcattcat gaaaaaaaaa
    30241 ttcaatatta caacaacaaa ctgcaagtca gggtacctca gatattttat aaaaattaaa
    30301 tgcaataact acaggcattc agtcaaatta gcataggcca aaaccaacca ttttacacca
    30361 tgttcactga attttacttg aactcttgta tgggccccgc tgccctcaat ggcaagaacg
    30421 acaccctgtc caaatttggc atgctgcaca ttttgcccaa ttgtaatgcc cgacacctcg
    30481 tcagccactg gcaatttgct ttttgtagtt ccgggccact gagaacgtga tttaactcgt
    30541 acctcatcaa gaaattgttg aggaatttca cgcaaaaatc ttgatgatct gtgatactct
    30601 tcacggccat attgtcttct gacctcagca taggataaaa ccaatttacg catggcacga
    30661 gtcataccga cataacataa tcgacgctct tcctccaagc gaccaggctc ttcaatcgat
    30721 tgtctgcctg gaaatattcc ttcttccata ccaaccaaaa acacaatcgg aaactcaagc
    30781 cctttggctg catgtaaggt catcaagtga acggagcgct catgttcatc agcctgcaat
    30841 tcccccgatt ctaatgaagc atgagcaagg aatgaattga ccaacggtaa ctcctcctct
    30901 tcatcgtact catatcgaaa ttgtttggca gcattaatta attcttgcag gttatccaca
    30961 cgtgattctg atttatcacc tttgattttg ctaaagtggg cgtataaacc actatgctgt
    31021 attacatcag agatctgctc atccaactcc ttattagcaa ccacaacctg taatttttcg
    31081 atcaaatcaa taaacttagc taatgccaag ctcccccgtt gccctaatcc tgtggattga
    31141 agtatgccct tggaagcgtc ccacaacgaa cactgttctg ctctggcgta ctggcgaact
    31201 tcatccaatg ttttttctcc tattcctcga gtaggaaaat taaccactct ttcaaacgca
    31261 gtatcgtcat ttggattaac taacaatcgt aagtaagcca aagtatcttt aatttctgcc
    31321 ctgtcaaaga aacggactcc tccatatatc ctgtaggcta tacctacacg taacaaagct
    31381 tcttccaaaa cacgggattg ggcattcgaa cgatacaaca ctgcaatctc atccgcactg
    31441 gcgccctgat tcaattccat gccaattcgt tcagtaacaa aacgtgcctc atccaattca
    31501 ttgaatgcac tgtatacaag aattttttct ccttcactac cagcagtcca taactcttta
    31561 cccatacgag tactgttgtt gtgaatcaat gcatttgctg catttaatat cattgcagtt
    31621 gacctgtaat tttgttcaag tcttatgatt tgggtatcct taaaatcatg aacaaattgt
    31681 tgaatatttt ccactttagc cccacgccaa ccataaattg actgatcatc atctcctaca
    31741 gccaataccg ctgtatgatc accagcaagc aaacggatcc atgcatattg aatcgtattg
    31801 gtatcctgaa attcatcaac taaaatagct tggaagcgtt cacggtaatg agcaagaatc
    31861 tcttcattat ctcgtagcaa ttcatgagtt cgtaataata gttcagcgaa atcgattacc
    31921 cctgaggttt ggcaaacgtc ttcatacgct ttataaattg aaaccaaagt cttggtcggg
    31981 ccataatgca gtgcattaat atgttgtggg cgcaagcctt catccttttt actattaata
    32041 aatgcttgcg cttgtttgac ttgccattgc tcaggatcaa gattaagtga aagaatgact
    32101 cgcttgataa ctcgtgcctg gtcttccgaa tccagaatat gaaactgttc tggaaggtta
    32161 gcctccttgt agtggcgacg caataaacga tggcataatc catgaaaagt accaacccat
    32221 aaacccaacg ttggtgtaga caacatactg ctaagtctcg atctcatttc accggccgct
    32281 ttattcgtaa atgttactgc taatatagcg tggggagaga gatgctgttc ttcaattaac
    32341 caggcaattc ggctcaccaa gacttttgtt ttaccactgc ctgcacctgc taaaaccagc
    32401 atattaccta atggagaggt taccgcgtca cgttgctttt cgtttaatcc cttgagaagt
    32461 gctgctatgg tcatagaata tttccaacag aaaaagtgca agtattatct tcaatttaat
    32521 tgctaaagca aagatataat cgatacggta aaaaatctaa aaattctcct ggatatatat
    32581 cgataaaacc catacaagta ttgctttctg atcaatcaga gcgcaaaata ctgttcgttt
    32641 aaagtagcta ctagatagta ttataataca atgacgttat cataaaggac taaaatgcat
    32701 accctctacc cagcaattaa accttacaca caacacgaat tgaaggttag taaactgcat
    32761 acactttatt tggaagaaac cggaaatcct gaaggcatac ctgttattgt attgcaccat
    32821 ggcccaggtg gagaagctga cccgcatcta agacgttttt ttgatcctca acgatatcgc
    32881 attattcttt ttgatcagcg aggttgtggc cgttcaattc cacatttgga aataagtgaa
    32941 aataataccc agcttctcat ggaggatatc gactccatta gagatttcct gggcttaagg
    33001 gaatttgtgt tatttggggg tggatggggt tctctgttag cattattgta tgcgcaaaaa
    33061 tttcctcaac aggtgaaagc attgctactc caccaaattt ttttaggtcg acaaaaagac
    33121 atcgattggt tttataagca tggagccagt ctggtttatc ctgattactg ggaagagttt
    33181 acgaacacag tccccgaaaa caaacttgat aatatccccc aatattatgc ggaatgtcta
    33241 caaggctcta atgagctagc gcgaatggct gctgcgaaaa actgggctct ttggcaagcc
    33301 agatgcagta gtttacagcc acacttatat gttattgatc agtatagcga cccacatttc
    33361 gcattagcat tagcaactct ggaatcctat tacattatta atcattattt tattgaagaa
    33421 aatcaggtta tagctaatgc ccataaaata aggcacattc ctacctatat tgttcatggc
    33481 cgttttgact tggtaagccc tttatccagc gcatgggaac ttcatcaagc gatccccgct
    33541 tccaatctaa gaattgtcag agatgccggt cactctgata gagaatcggg aattattgat
    33601 gcacttattt atgccagtaa ggacatctca aagcagggtc ttgatgcatg ttaactgtat
    33661 tacaaagagt aaaggaagcc agggttgata ttgatggtca aacagttggc aaaataaatc
    33721 atggcttgct gatactttgt ggttttgaac ctaaggattc gctagaaaat attaaacgaa
    33781 tgctggataa atgcattaat tatcgcattt ttgaagaccc gagtggcaaa atgaacttga
    33841 gtttaaagga tgtaaatggc ggcttgctat tagtaccaca atttactctc atggcagata
    33901 cccaaaaagg attgcgtcca agtttttcaa atgcagcttc tccagaactc gggcgtgagc
    33961 tttttgataa cttattaact cttgctcaga aatgccacca aaatactcaa agtggttgtt
    34021 ttggagcgaa catgcaagtt tatttatgca atgatgggcc tgtgacattt ctgttacaat
    34081 tttaattagt ttttactgta ttaaattttt gtataacctg aattgtcgag caaaatacaa
    34141 tcaacattca ttattggaat tcattccggc caaaaaaaag catgtttgtt tcagttaaaa
    34201 agcagcaatt taaaaggcct attctttaca tcaaagcatt tgtttgcaag aaccaaaaac
    34261 tggtgtaaca tcattttaag tatttaataa atagaataat tatgcgacta atcttaatgt
    34321 ttgctagcat catgggatcc gttattttag cgagctgtgc aagtaaagga tcaaacccga
    34381 tcgatccatt cgaacccttt aatcgtaaag tgcacaattt taatatggca tttgatgcta
    34441 ccgtattaag accaccagca aaattatatc aggctgtcgt accaaaattg gtaagaaaag
    34501 gtgtgaataa tgcattcaat aatctggatt tgttccctac aatagccagc gatatactgc
    34561 aagctgaagg aaaatgggta atcaaagaca gttggcgact cttcattaac tccactattg
    34621 gggttgctgg tctgtttgat gtagcagaaa aatttggttt gccaccgcat tacaatgatc
    34681 tgggcctaac actcgccaaa tggggcgata aaaaatcgcc ttaccttgta ataccctttt
    34741 taggccccag tactcttcgt gatggagccg gctggctgtt ccagtttgca ctctggagtc
    34801 cttatgtata tattgatgat gctggtctgg tttatggttt aattggatta cgctatattg
    34861 atttacgaac acaatttttc gactctgagc ctttactgaa tgaagcatta gacaaataca
    34921 cattcatcag agacgcttac ctgcaacata gaaattatct tattgcaggg actgagcaag
    34981 atacaggcac gtccttcgtt gaggataaga aagctaacaa aactgcaatg caagaatcaa
    35041 ccgcttcaaa taaccaacta ggttctgact atgttgatga ataaaatgat taattgagac
    35101 ttgccaggtc atgataaggg ttggtgttga gccaaatgca aaaccaaccc tctttaagct
    35161 ctgaacttca aaatcaaaat aatgactgtt ctttattttg ataaagaagt atgtaacttt
    35221 tcaaggcagc gcatatattc acctgcgata tccaaaccag tttcagtgct aatttcccga
    35281 atacaagtgg gactagtcac attaatctct gttagataat cacctatcac gtcgataccc
    35341 acaaaataca gacctttttc tctcaaagct ggagctattt gttcacatat ccatttgtct
    35401 ctttcagtaa tagctaccac ttctcccctg gctcctgcgg ccaaatttcc ccgcaaatct
    35461 ccttttgcag gaatacgagc caaagcaaaa ggaacggggt cgccattgat caataaaatg
    35521 cgtttgtctc cgcttactgt aatatccggg atataacgct gagccataat acattttgtc
    35581 tgccctttgg ttaatacctc caaaatgacc gataaatttt taccttcttt gtcaacataa
    35641 aaaacggaat tacctcccat tccttcaaga ggtttaaaaa taacttgttg atgaatttgc
    35701 cagaactctt ttaattgccg agcatctctg gatacaaaag tagggggaca gcaatgagga
    35761 aaatttaggg taaaaaattt ctcattcgcg tcacgtaaac tttgcggttt atttgcaacc
    35821 aacacccctt cttgttccgc taaatccaaa gcgtaagttg cataaatata ctccatatca
    35881 aatggaggat ccttacgcat caaaataaca tcaaaatctc tcaatggctt attacccaaa
    35941 tctatggttt taacccaact cagactctct atatctccaa tgtgaatttc atggactcgt
    36001 gcataagcgc ccccatcaca acaatataag tcattcaggg taaagtaggc gcaagaccat
    36061 cccaatcctt gtgcgctttt aatcatagct actgtagagt ctttataggg ttttatttga
    36121 tccaatggat ccataagcac cgcaagtttc atcattcgcc tccaattgca gcaatttctc
    36181 gagctgcagc cagtgcagcc aaacgagcta taaccccata aacataaaaa cgatttgggc
    36241 agtcaactac ctctaaatca tcacatggca tattacaagc ctgtgcgaaa gctaacggct
    36301 caaaatgcat gcctggagca ttgaggttct catcaattcc tctgccttta tgaacgcgat
    36361 aaaatccacc aacaacaaat tgaccaatca tataaactac aggttcagca acagaaccat
    36421 caggcatcgt ttcaaaagta taaacacctt cctgaataat gactctgtct accttcctgc
    36481 ttcctttact ggtagacatc ctggtacgct gctttctatt caattgtcga atttcatctc
    36541 cattatgaac catcataacg ctcatgccat aagtaccatt atctgccttt actactgcga
    36601 acggtttatt ttctattccg taagtagtat atttatcgtt tattaacgat aatatcttat
    36661 cgacttcttg agctaaggtt tcaatccctt cctgggccat aaaatcgacc ccctctatag
    36721 cgctaaaata gggattaatt aaccacggat caataccgac taattcagca aactcaatag
    36781 ctacttcttc aaaaaattga aaatgactcg attttaatcg agaagcccat cctaatttgg
    36841 ctgtaggtct aattcgctgt tgcacatcct gtaatatttc aggaacgcct gaagacaaat
    36901 cgttattcaa taacaaaagg caagggctga aattatccag tcccacctta tcaccttgtc
    36961 tttgtaatgg ttcaattaat aaagtttcac cttgttcgag tattatttct gtaggttctt
    37021 taatgtctgg atctaaactg cctattcgaa ctataaaacc agcttttaca aaaattgttt
    37081 ttaatacatt caaactctgt aaataatact tattccgagt atggctttct gggagaagca
    37141 aaattttagt acaatcaggg atatactcta tcaaggcaga ttgagcagct tgtatacaca
    37201 ggggaaggaa ctccgggttt aaattattaa aaccagctgg aaataaattg gtatcgacgg
    37261 gagccaattt aaagccagca tgtcgcaaat caacagatga cgttagaggt gccggtgttt
    37321 cctgccactt ttttctgaac caggattcta tttcagcaag tttatttaat attatttttt
    37381 ccacatgata caaaggacct gaatgagcag tagtcagatg tggaacgggg atttcatgct
    37441 tattcattca tactccctat tttttaaaat ggatcgatgt tacctaggtt attcataaaa
    37501 taaaagtatt tatcattctt aatacattat t
//
LOCUS       DASLOE010000014        36542 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.014, whole genome shotgun sequence.
ACCESSION   DASLOE010000014 DASLOE010000000
VERSION     DASLOE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 36542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36542
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.014"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..979)
                     /locus_tag="U6309_000556"
     CDS             complement(<1..979)
                     /locus_tag="U6309_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504011.1"
                     /translation="MRIATLFSAVLFISPLWAAPKFVPSQPANGVYDIKGTELCSTAK
                     HTLAYLNKGKNYDPQVIHDGKVIKISLDRVKKTLEFICQNQKKLNNPDFVKQHFDFIR
                     WYPDLSQAKQLSANKPLLQNIPKDKILMTKYFVHLAGASTKPSSAKPYPLYALPEDEQ
                     NLTLDEANAKPGLIRFRYGKQAILSGALKNQPVSKLAYLSRDDLEAALLQGTVVADFG
                     PTAGKKIFNVHRSNNIAYDRTKTPYQQERFWYFKEVDGIKGYGKDAEYKITVNPKVTF
                     AADLAEFGLGKLLMIQYQDKSGRTMTKMGILADTGGAFNNNLYQIDYLAG"
     gene            complement(980..1672)
                     /locus_tag="U6309_000557"
     CDS             complement(980..1672)
                     /locus_tag="U6309_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HEN5504012.1"
                     /translation="MRKQALHPRTAVINKAQKNQSKRARSDALSWLAANFPEAFDNSL
                     RIRPLKIGIMSDILQHAEKAEQVGISKSKLREAVVLFTRRLDYLACLKAREVRIDLHG
                     NPVEEVTEEEAEHASMKIKKRVEKSVKNARKLVNAKAANHSHVNNQPSTVSSVKPMNS
                     FDSHPEPLLPIYPLRSSTYASQNVAMQSAKSPSVVVKHKAPKQYDPDAVARLKEKLGL
                     SRKAEDKKETTE"
     gene            2029..2214
                     /locus_tag="U6309_000558"
     CDS             2029..2214
                     /locus_tag="U6309_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504013.1"
                     /translation="MRIILSILAIFIPWLVLLIKDNPGGAVVALIMQATLIGWPFASI
                     WAWRAVNPLTPASQSKQ"
     gene            2394..3125
                     /locus_tag="U6309_000559"
     CDS             2394..3125
                     /locus_tag="U6309_000559"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEN5504014.1"
                     /translation="MRTRVIVNGANGKMGILACETLENHEQFEVVAKLSRQDNLGRSI
                     LDTKAQIVVDLTRADCVYENSLTIINHGARPVIGTSGLVETQIDELTKLCEIKQIGGI
                     IAPNFSLGAILMMMLATKASEYFSEVEIIEGHHQQKLDAPSGTALKTAEMIAAARKKP
                     KNKLPLKELTPGARGGSHHDINIHSLRLPGLLARQEVLFGNIGETLSITHNSIDRRCF
                     MPGIVLACQKVLNLTNLVYGLEHLL"
     gene            complement(3229..5697)
                     /locus_tag="U6309_000560"
     CDS             complement(3229..5697)
                     /locus_tag="U6309_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN5504015.1"
                     /translation="MGKGIILRVLENTILSPQVFDTLERLLPGYKLEYFKEQPDYRKS
                     IARRIDSLHDAFSFILKAYPLDPKHTSLTVATLSTYAAECKASCDLEKLTLEELHLEL
                     ERFTAKLVEAIAIAWKWPKGKAVKEAIASLNEAEQYVLMSRGRSDIATIMPIEMGSET
                     KYVLQYDESLSPVYEQWLTELKQLKEYSFPKTPAWFKNLPPYQQAYYCNLNLSSVDPK
                     KALQHFNTFFGNWGDIAKRSLNLTTELNQIHTNSPPYPSWFNELSPAQQAMIRVLSAT
                     PHEIKSSLKEFKKFMVEQARNDQYASTLSLVPKLPQWYWVLSEKQQYFLEYALKNAEK
                     VEDVVSYLSSRHRTLPAPANYGAHSLYLIDDEGKETLFYDKRYRSSHVASRDSLKFPE
                     DVQQRHVDSNLVKVMEFAKPQQPLLLQTLISPIHAVDYIPTVVTDFLPELPPDLELYK
                     IAREAVTRSKRRHEIFQHNHPFNIAKRYYYTQATDTDSEFLLKAAQKYASSKPGLQAL
                     IDDYKAVLESPLGSATFWDYDGRELFLSSLEELIILNMGGYSYGSCVSGKDRKAVELL
                     HTDAMILYKAKYGNWPKFGIPKEKQERVNFINIVVDLYISRHQHELAGQNAPGSEGIK
                     TPDWYWPNDIAEAINERLGTEKALAYDDRLATDNEVKNISKDLRSFFLPENELHCLLI
                     AKQLGEKMCTMLYDVLSALINEERRFQKSSKDSWKLRWFSDKDVSSTPTGILNIREVM
                     HDENSGNDNVLRIGKIFAAVLNRPESDSSRTTATNSVYDRIRKLLQPLSSETTLQTLA
                     EEAILEWSSLFESSKRENSGLVYM"
     gene            5875..6051
                     /locus_tag="U6309_000561"
     CDS             5875..6051
                     /locus_tag="U6309_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HEN5504016.1"
                     /translation="MKSIEDFTGFSESGIDDAIQNALKKAGNPTHFEVVETLGSQDSQ
                     SDRRYRVTLKTLSD"
     gene            6162..7661
                     /locus_tag="U6309_000562"
     CDS             6162..7661
                     /locus_tag="U6309_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEN5504017.1"
                     /translation="MGYNVPHYIGGQLINETDTEAQAIYNPALGEIIGQVHFASKATC
                     DRAVAIAKAAGNEWSQTPAIKRARVLFRFRELLEKYQMDLARIVTREHGKTLDDAKGS
                     VARGIEVVELHCGLVNELKGDFSAEVASHIDCHTFRQPLGVCAGVSPFNFPVMVPIWM
                     MIPAIACGNTFILKPSEQDPSAPVRLLELLSDAGLPPGVANCIQGNKTTVEHLLAHPD
                     IAAFTAVASTPVAEYIYTTAAAYGKRAHTFGGAKNHCVVMPDADLDQAANAIVGAAYG
                     SAGERCMALSVVVTVGQHTADALINKISPLIRAIRVDAGDVEGTDMGPLVSQAHRQRV
                     LAAIEKGVNEGARLIIDGRTFQHPQYPQGFYLGPCLFDEVTEEMSVYQNEIFGPVLVI
                     VRVNHFEEALALVNKHQYGNGTAIFTRDGFTAREYSQRVQAGLVGINIPIPVPIANHP
                     FGGWKRSVFGDTNMHGEESIHFYTRRKTVTSKWPITELKDNSFAMPTHD"
     gene            7685..8581
                     /gene="mmsB"
                     /locus_tag="U6309_000563"
     CDS             7685..8581
                     /gene="mmsB"
                     /locus_tag="U6309_000563"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEN5504018.1"
                     /translation="MANIGFVGLGHMGFPMAINLLKAGHSVMGYDLQQSALEHFAEQG
                     GAIAHNLQELAKGKDVIVTMLQTGQQVLHVCLGVDGIFSQLNAGSLYIDCSTIDVKSS
                     RETHQIAKENHLLVVDAPVSGGVAGAAAATLTFMVGGEAEAYNAAHPILSAMGKKIIH
                     TGGAGSGQAAKICNNMILGTTMIAISEAFILAKELGLSDGKLFEVVNNSSGQCWAMSK
                     YVPVPGILENVPANNDYKPGFAANMMLKDLLLSQNTAKSVHIETPLGAKTTEIYQQFV
                     DQGLGDIDFSAIIKHIGKTQEV"
     gene            8583..10466
                     /gene="acs"
                     /locus_tag="U6309_000564"
     CDS             8583..10466
                     /gene="acs"
                     /locus_tag="U6309_000564"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEN5504019.1"
                     /translation="MLPVHLRHPHLTPTEESLSEFWSEIAAQTIDWIQPWSITLQGGL
                     HKGDVKWFQGGLLNVSANCLDRHLPHKANQTAIIWEGDDENQNKTLTFAQLYSEVCKM
                     SNVLKSLNVRKGDTVGIYLPMIPEAAIAMLACARIGAIHTVVFAGFSAHALQQRLIAS
                     SCKCLITADAFQRGGKTIPLKKQADEASVDLNITKLVVKNSNDPTALNKNKEHWWHEL
                     KQTVSDQCMPEPMNAEDPLFILYTSGSTGQPKGVVHTTGGYLVQAAYTHQLIFACQDN
                     EVFWCTADVGWITGHSYVVYGPLCNGTTTLMFEGIPTWPDSARNWRIIDKHQVNVFYT
                     APTAIRSLMRAGDQWLNSSSRSSLRLLGSVGEPINPEAWNWYHQKIGQGKCPIVDTWW
                     QTETGAIMISPRASDEVIKPGSARKPIPGIVPLLLNEQGHEINGAGEGLLAIKYPWPS
                     MARTIAGDHQRYCNTYLSNGYYITGDGAKRDEDGDYWITGRIDDVLNVSGHRLGTAEI
                     ESALVSHPKVAEAGVVGIPHDLKGQGIFAYVILKQGNKPDAKLQTELMERVKDEISAI
                     AKPDVIQFVNDLPKTRSGKIMRRILRKIACKEVSHIDELGDLTTLANPQIVEELMKNI
                     LKQ"
     gene            complement(10563..13874)
                     /gene="cegC2"
                     /locus_tag="U6309_000565"
     CDS             complement(10563..13874)
                     /gene="cegC2"
                     /locus_tag="U6309_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC2"
                     /protein_id="HEN5504020.1"
                     /translation="MTPLFPTQGPITIRQGIGGSCYLLSSLDCILNLGADGEQLIKSL
                     FTQTEDGKVIVRIKRHEALKDNLQKNKMTGKYTHYVDELNNEDVFEISPERLKEIDNQ
                     YGGVKSNSLAIKILERLVSYYYAGDWSNTDPLASVVAHDIPDRIAGFTSTAFLGKFFG
                     IQAEDIPYSKLDDIIKLKLMNPDEPVYISMSYGKVDSFGKFHGRHALRIDKIIPKGSG
                     NYDFVLINPHDNSKTETYKLDDLNKRNCRFCLFNTSIHRASLTKKLLTLSNEEGRYVF
                     SNSGLQKRLISLEEMNLLTNNKIISSCISLHKQIPYLEKLFLKLSVEEKKTLTTCIAN
                     ADGSKKEFLKLFLTHIPAMDLLELVLREETSQELLGEVLTELALSNPVEENKVSPKAG
                     INFNGEAFLHLIVKSAIQQKINQLAYMPEKAKQEIESGLINFYFGGASSSLTRASGLR
                     ALFIANVFSKKSIEALFPPKALFAKAIANYLALETLPDLLIEYLKSKDTSPIDEEFFD
                     VVLASATFKDPDEFFENLFRLSRINPEVAKALFVFASQKINLLFGISLEEYAKKIALK
                     DSGEFKSWFESLSKPQPVIKIPEIDNVLRQQRVDDAKRVISDIVQRINSFPFSFEGFK
                     TVAHVNLNAEELRGQLKKIVHSGELQNVLQILDLPDGHPEVQRALERKLRMIDAAANR
                     RSDFLRKYETDIDEHVRQIKNFPIDFNDADTIVAIESQRILLNKKLHTLVKAEDLLGE
                     QFIANPKIKMVYYAQVEKINLRAEVLQKQLLDEAQKVIDSVEKRIDNFVIRFDDISSA
                     SAVEWQRNNLLQQLDNLVKPNQALLSAEKVLDCNDLQPSIVRALQAKKQEINETADQL
                     IIKINAEEVVKSYEKQIREFPISFNRCQTVEEVIARKQDLIQSVRNLVGNKPDLLKAQ
                     EQLQLLSGEYHSDIKMALTDKVHEINRQADAVSKRITDQIAATTETLNILAEIKFSDH
                     LKTIESMVKTLETKAVGDENYKRAAPIARTFYNNLLRAEERFKNSQLPKNVKCKDFHQ
                     DCVRAINAVIPVLEVHRGWKQVFADLASALVTLCTLGGANLYAGRWRLFPVPTESEKI
                     VKDFSLSMQPLAVRA"
     gene            complement(13988..14590)
                     /locus_tag="U6309_000566"
     CDS             complement(13988..14590)
                     /locus_tag="U6309_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEN5504021.1"
                     /translation="MPINLYSKAVFLKSAARVNQLPEDSGYEVAFAGRSNAGKSSALN
                     CLTNNKNLARTSKTPGRTQLINLFSLDEQRRLVDLPGYGYAKVAMEVKLEWQKNLAHY
                     LEARQCLRGLILLMDVRHPLKDLDQILVNWALHRELPVHILLTKADKLSRSEVKNAVL
                     KVRQYYELAEHLVSVQAFSSVKKDGVEELISVLDRWYEWN"
     gene            14665..15267
                     /locus_tag="U6309_000567"
     CDS             14665..15267
                     /locus_tag="U6309_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEN5504022.1"
                     /translation="MKKLALVLILCSSSVIFAQENPQEAGQNKSTVCTACHGPQGIST
                     NPEWPNLAGQHEKYFVKQLKDIKEGKSRSAPTMTAIVANLNEQDMDDLAAYYAKMPVA
                     EGSTPEKYLKRGEQLYRGGDLNKHIAACIACHGPKGSGNAQAGFPLLSGQHAAYTIMQ
                     LQAFKDGKRTNDLNQIMQDISSKMSPEDMEAVAYYIQGLY"
     gene            15278..15892
                     /locus_tag="U6309_000568"
     CDS             15278..15892
                     /locus_tag="U6309_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEN5504023.1"
                     /translation="MFKKLIALLFLIPMTALATQFIEGKDYQTVASAQLSTNKDKTPL
                     ITEFFSYGCPWCYKIDAPLNDWATRMGKGAYLERIPVVFKPNWDLYAKAYYTAKTLAM
                     SDKMNAILFKAIQEDKNPLATKQSMVDFFVAHGVDREIAKSAFENSPTIDMRVNSGMS
                     LMAHYQINAVPAFVVNNKYKTDLQMAGSEERLFEILNYLVRKSA"
     gene            complement(16053..17351)
                     /locus_tag="U6309_000569"
     CDS             complement(16053..17351)
                     /locus_tag="U6309_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN5504024.1"
                     /translation="MPETIINIENLSKSFKKAPSQHLLVLEDVNFKLQEGEIVALLGK
                     SGSGKSTLLRIIAGLIAPSSGTVTYRGKPVTRPVEGIAMVFQSFALMPWLTVLENVEL
                     GLEAQGISREERRHRAIEAIDIIGLDGFESAFPKELSGGMRQRVGFARALVINPDVLL
                     MDEPFSALDVLTAENLKSDLLELWKEKKTNTNGILLVTHNIEEAATLADRIVIFGNDP
                     GYIRAELPVTLPQPRDPESPEYLALVDKIYTLMTTGPKEKAKRAQRERQIGLGYRLPD
                     VEPSELSGLIETMKSFEERIDLPELADELMMNIDDLFPILETLEILGFAKVSAGDIQL
                     SELGKQFSEADLQERKQLFAQRLLEKVPLARYIRRVLDEKAGHRVSEERFLSKLEDYL
                     SEKEADRVLKTMIDWGRYAEIFAYDFNTGILSLENPGKGA"
     gene            complement(17359..19098)
                     /locus_tag="U6309_000570"
     CDS             complement(17359..19098)
                     /locus_tag="U6309_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN5504025.1"
                     /translation="MGNSRFYFANPDKFSRFINRWDLLLLILTFSVLFFLGWAGSQMA
                     TPYQLGDQIPISLEPSNLPFYALRTVLRMFIALIFSILFTFIVGALAAKNRRAEQIVI
                     PAIDILQSIPVLSFLSITVTGFIHLFPNSLLGPECASIFAIFSAQVWNMTFGFYQSLK
                     TVPHDLIEVSAMFRLSAWQRFWKVEVPFSMSGLLWNMMVSMSASWFFVVLSEAISVAH
                     QNIRLPGVGSYIALAIAQRDLHAVGYAILTMVIVIFLYDQILFRPLIAWSEKFKMEQS
                     PDESEYQSWLIDLIRSSRLMKRVTEWLAVFTNSFVNARWLRIGEVKAVKEIDFKRQKR
                     LDRLWTALVLIAVITGGWFLLKFILAELKVSDIFHVFLLGAATGARVIILILLSSMLW
                     IPVGVWIGLRPRLAQKIQPIIQFVAAFPANLFYPLFVIAIVKFNLSVEIWVTPLMILG
                     TQWYILFNVIAGASSIPRDLYLAADNFGLKGWIWWKRLALPGIFPFYITGAITAAGGA
                     WNASIVAEYVSWGNITLKATGLGEYIQASTTAGDFPQIALGTAMMCVYVLAFNHLIWR
                     PLYRLAEERFNFN"
     gene            19595..20125
                     /locus_tag="U6309_000571"
     CDS             19595..20125
                     /locus_tag="U6309_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HEN5504026.1"
                     /translation="MNKTITIALSLLLSIASKLTYAGPDRAQLAVWANEAIIATYTFD
                     YKNYMQQQKEIAKYFSADGWIAYSKALNQSKLPEVVQKNAYFVNAVATEPPKLITLDP
                     THWQAIMPILVVYKNPQYEQKQNLKVILGFTVASPGQGVRGFSVTSLQSTPISPPCQC
                     KIEETPGNTKQGDAKQ"
     gene            complement(20100..20567)
                     /locus_tag="U6309_000572"
     CDS             complement(20100..20567)
                     /locus_tag="U6309_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEN5504027.1"
                     /translation="MVWPIIWRWILDLGWLCFLLILFWYFWKKRRDLVEARSWLKAKG
                     HVTRCEWTRVGHSVWPRIEYIYEVYEKDLTGEYLFLDTTFNTPNSKYSRSIAYKVAIA
                     YRDNSEIDVYYNPNRPEQSALDVTIPKKLTFILILISSLILLHIGIIVWRLLV"
     gene            20703..21785
                     /gene="gcvT"
                     /locus_tag="U6309_000573"
     CDS             20703..21785
                     /gene="gcvT"
                     /locus_tag="U6309_000573"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEN5504028.1"
                     /translation="MTAKTPLHTTHLACGAKMVDFHGWDMPLHYGSQLNEHHAVRNDA
                     GMFDVSHMTIVDILGAGGRQFLRKLLTNDVDQITHNGKALYSCMCNEHGGIIDDLIVY
                     QRASDNYRVVLNSATRQNDVAWIRAKSEGFAVGLQERRELSMLAVQGPNAIAKTLSIL
                     APAHVDAVSTLTPFECVDVDHWFFARTGYTGEDGLEIIVPNEFITQLWNDLLNAGVTP
                     CGLGARDTLRLEAGMLLYGQDMDETTTPLESGLAWTVKWEPEDRGFIGMGALVSQKQQ
                     GIKRKMVGLTLLDKGIMRHGQKVIIEGCPDGIITSGSYSPTLQQSIALARVPVETGEQ
                     VLVDIRGKLIPAKVGKPRFIKQGKPV"
     gene            21819..22196
                     /gene="gcvH"
                     /locus_tag="U6309_000574"
     CDS             21819..22196
                     /gene="gcvH"
                     /locus_tag="U6309_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEN5504029.1"
                     /translation="MNDLKFTITHEWLREDEEEVTVGITDHAQELLGDMVFVELPEIG
                     DEVSAGQELGVVESVKAASDFYAPISGVVTAVNEAVGKNPALVNHDPYHEGWLVKLKP
                     SHPDEIKSLLSDEQYQNEIAEEN"
     gene            22199..23569
                     /gene="gcvPA"
                     /locus_tag="U6309_000575"
     CDS             22199..23569
                     /gene="gcvPA"
                     /locus_tag="U6309_000575"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEN5504030.1"
                     /translation="MPYIPHTPNDTKEMLAAIGAQDIQDLFDEIPASLQYAGFQNIPA
                     GINEMEMLKEAQNQAQKNRNGICFIGAGCYEHHIPAAVWDIASRGEFLTAYTPYQAEA
                     SQGTLQLLYEYQTMICELTGMEVSNASMYDGATALAEAVLMAVRLNKHSKTNRVLIAG
                     TVHPFYRETIETIVRNQHIEVITLPFDEQQGITDLGSLNQYTGEDITALVIAQPNFFG
                     CLEQVDKMTSWAHHNKTISVACVNPTSLALLKPPGSWGEHGVDIVCGEGQPLGSPMAS
                     GGPYFGFLSTRMAHVRQMPGRIIGRTVDKDGKTGFSLTLQAREQHIRRAKATSNICTN
                     QGLLVTAATIYMSLLGPEGLSQVATQCHQNTHELISALTQIEGVELAFKAPFFHEALI
                     KLNQPVQSVLQQLADAGIAGGYAPEQHYPQLANTLLVCATEVRTAEDIAKYAKTLKTI
                     MSKRGA"
     gene            23569..23877
                     /locus_tag="U6309_000576"
     CDS             23569..23877
                     /locus_tag="U6309_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase"
                     /protein_id="HEN5504031.1"
                     /translation="MFIVQLTYLVPINEVNKYLQAHREFLDYHYKQGLLVASGPMKPP
                     TGGIIIAATNDRAHLESVFKQDPYYLAEIAEYQFIEFTPVKHRNELKELIQKTEGKLC
                     "
     gene            23871..25325
                     /gene="gcvPB"
                     /locus_tag="U6309_000577"
     CDS             23871..25325
                     /gene="gcvPB"
                     /locus_tag="U6309_000577"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEN5504032.1"
                     /translation="MLIFELSKTGRQAKAQIPRAVSKNYSIPEEFQRKSPPRLPACSE
                     LQVVRHFTCLSQKNFSIDTNFYPLGSCTMKYNPRGVHKAASLPGFINRHPLAMDNESQ
                     GFLETLYKLQNYISEITGMPGVSLTPMAGSQGEFAGVAMIKAYHQSRGDTARDEILIP
                     DAAHGTNPASAVMCGFKVVEIATAPDGDIDLDELKRKVGPRTAGIMLTNPSTLGLFMR
                     QIKEIASLVHQAGGLLYYDGANLNAILGKVRPGDMGFDVMHLNLHKTFATPHGGGGPG
                     AGPVAVGKRLIPYMPLPVVKKTDSGYHWATRQDYPQSIGRLSCFMGNAGILLRAYFYM
                     LVLGKEGLLRVSEFATLNANYLLKELTKVGYTAAYPDRRASHEFILTLNSEKKNYDVT
                     AMDFAKRLLDYGVHAPTTYFPLLVPECLLIEPPETESKEELDAFVAVMKTIREEASKQ
                     PDILKAAPHTLPVKRLDDVKAARELDLNYFATHE"
     gene            25530..26774
                     /locus_tag="U6309_000578"
     CDS             25530..26774
                     /locus_tag="U6309_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEN5504033.1"
                     /translation="MVVELDEPPVRTLLARQGIYDKNSAIFAYELLYRNSEAQNSQVD
                     NLNPLSGEAATSSVLLQLFANLDVNAIIGNKQAFINFTHNHLVQKIPMLLPKNRIVIE
                     VLETVAIDQALLFNLMELKKQGYQIALDDFVFRNELAPLVDMADIIKIDVLHLDQQQI
                     AEQLLPLKSFRGKLLAEKIEDKQQFNHCVNLGFDFFQGFFLNKPDSLTGQIITENKIQ
                     LLRLLTEINNEEVPIQRIEEIILQIPKLSYRILRLANSASLYMSKKIESLMDAISQLG
                     LMQIRNWLNLLLLASLDDVAPDLLERTLIRAKMCESLAKSMDYPNSHQAYTVGILSTL
                     DGILNEPMPSLLAKIRLSEALNEALLNYKGDLGKILKFAIDYEQANFNQLERIPVKSE
                     TLSQSYLKGIEYANHVIDIINK"
     gene            27011..30229
                     /locus_tag="U6309_000579"
     CDS             27011..30229
                     /locus_tag="U6309_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504034.1"
                     /translation="MIIRELFNWIHNDRATLHLSDQTIERDLIEQEESQAKQTHRVLL
                     GNVRLLKYSGSSNTEAIKELEQHCLFMDYLQLYKQEFVKDGKNTVFLIALRNLLSQSH
                     EEQLRLMPKINELFRVLLRDNKESALSFYDKNKELFRHCTEIQERVTFELLQQKMEAD
                     SKSVMTQLDYLNKHLASQENPLGIIALCRNWIGETEKFTALILWLLERKVNVEKILLT
                     NLLQDFLKYHLFTLHSKDNEVSRLYSLLGHFPEAQELVMAAQKISCGELMFQQYSLDG
                     IFRGKNLPVVSPEIPPLQFSLKKDNFNALYQTFGQDFLTAGVATATNHSEVAWLDMLR
                     HTLNQPETLKLLPDIINIIAREYSPKILNTLAELIAESTAHQLLTLNQSCVFHLLQHK
                     PRLLHDITEENVIGYINHLVRLDTHDQEIIYQLMALFRVLLKKNHPATKAVFEAILDN
                     LVNHPQLLEDEELLTQLKKYPDCELLLEERCENILKQLNFCIAEQTSGLLFGKHNYYI
                     IEDVWLGALRKFAVLQQINPQMKFSFGHKYALQARIAEVVFIHQGDLFDLDTFMDALD
                     LPPVTSSGEISLYERALIEILATIDNELIRKQIIQKLETIPFNRLDWHEREYGNQTVF
                     IKAAKKGNLGLINLLEDKMKPSVLNKALRVAAKNYQWETLAHLCSLPEVELSQEEMDD
                     LVAHLAKHGRVENIKKLLKLYDYKPSTELISAILKKGIENDNLQVVMYFCKLPVESPK
                     QAILDRLFKLAIQLQHWDIVKFLANSKHYSPSQTTLEKAFQQTALAMQHEAVEILGNV
                     EKTPVRAIVIERALLKASKLGHTNIVQSICSLPSEVLTKRAIEDALEQAAAQGHLDIV
                     SCLCEPGNTALRPPGINSGMKIAVQAGKLSLVNYFCSMTGSNKPTPRLIDQTLVMSAK
                     NGQTAIFMAIHSNRQTPPGKHAIEQSFQLAITGGKLQILDYLCRHERYGLNQSKIDQA
                     LISAVKSKQLEIVSYLCESLEITPSRKALRIAVTKAISSDQNDLAGYLRSCSSGKSKP
                     VDTPIDEIDSPCEIGKQLATNGLFKYKRKDDKGPPLLLNPSL"
     gene            complement(30531..31571)
                     /locus_tag="U6309_000580"
     CDS             complement(30531..31571)
                     /locus_tag="U6309_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEN5504035.1"
                     /translation="MVMRSFSFYYQHLENVSRSFSFCISQLTSPAKEWVALSYLLLRV
                     ADSIEDAKWQDLQTQSDSFAAFKSFLVQTPANDQFTKWLSSFPSQLPLGEKQLLCAVP
                     LLLEEKDNLPESIKQSVIKTILQVVDGMHYFLNHYHMEGKIVFPSLVTTNQYCFFVAG
                     LVGKLLSHIFTDLLSNFKWTASLLNQAFHFGFFLQKINLLKDKVDDETSGRYFISSEN
                     SLRESLVIHAHHSLAYVKSIPIIEGRPYRLFCAWSLFIGLASLKWLDKYGHRQKIKPR
                     ETYYLVNQINLLIDDNRALEKLFNSYLPGQYEEGSCYASESTKKIPTWFKKIYDDEMD
                     SLILSELDVQVW"
     gene            complement(31571..33373)
                     /locus_tag="U6309_000581"
     CDS             complement(31571..33373)
                     /locus_tag="U6309_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEN5504036.1"
                     /translation="MIQLGDLLKPTWAAEQSLNNAWSVLNDEEKETIKSRMEKIFYNE
                     IPFQLEHDKLIYIHLFSLFAQLETIGLRGLIKSLEKLRGTDLYQQMRQQITDEIFHAT
                     VFAKVAFQLSAPYALPLGHQKSIKHFISSLEGEEDLATSITLVNLVGEGWVEELCIAM
                     KEKNIAVTIFETVLEDESRHMDEYDLYRQIGLPNKDYLRKKLAIFEDELINTVFAHEQ
                     YLTTLGILLGKEGALKLLNNINNKHHWMLKKIGLTPSAHWQLFMDTMPLLMKNLSHDF
                     EKDKAIEPTNIRKLLSAIWNDPELPTESAIFNINVTPVCFFEKKFKPETITCLMLQAL
                     SKACFDNPQTRNYIFNHKLYHSHNSYVALAVKIPGSDQLGAIELKNCHEMTMTELAQH
                     IQHDMRIMKYCYEKTQSLQKEHPYLIEVVNRLLTPRHERVYRDFLFARPAISLSNIGH
                     WGYQAAVSPLFPNETFKITLTEIERKQVWNKTNNTFEVQDVLPVGMSVDHRVFDGNIP
                     FPRYMQEAFDQMFQDMEQSRIKPLSKPFSNLDNFIKYSNTLLENDLEFGFLYLFSLMH
                     VWKNYISYDELSKTVEENYERIKQALSKSEHQLG"
     gene            33981..34250
                     /locus_tag="U6309_000582"
     CDS             33981..34250
                     /locus_tag="U6309_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504037.1"
                     /translation="MNSKVFSQRFNRELATLGFPEDLTEKTKAVSKVFGVTRHLANAM
                     IFGHVLPDKEQLDKIAEILEVCPQWLSGATDRKKAYSGRETADSV"
     gene            34349..35155
                     /locus_tag="U6309_000583"
     CDS             34349..35155
                     /locus_tag="U6309_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HEN5504038.1"
                     /translation="MECLSFCVAKTIDLTRLDLHLKNVSKEFSAVKTRDVIRLNSHRN
                     KDHTVFIFKNGTVVSWGVKRYQIHEYLDIIKLLVDKPVALLVHDEFHYQIGDKTAIEP
                     HGFYDVDCLTIEEDSDELKLSLSYGFSQSVKLQYFETIIDALIEKYNPLIQALSHKGE
                     MPISRKQIQQVIGEILGAKSELNLISNFLYHPKYFWQHPTLEEHFSMLERYLHIQRRV
                     NAINHRLDTLNEIFDMFNGYLENRHSHHLEIIIIVLITVEIIIAVMNFHF"
     gene            35173..36453
                     /locus_tag="U6309_000584"
     CDS             35173..36453
                     /locus_tag="U6309_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN5504039.1"
                     /translation="MPTLKSYFLKDTSLRTEILAGATTFLTMVYIVFVNPKVLHDAGM
                     DQGAVFTATCLITAFACALTGFIANTPIGVAPSVALNIYFSYSVVQGMGVDWQHALAM
                     VFISGLLFLFLTLTKLRRLLIEAIPHNLQIAIVIGISLLIALIALENNQIIIGDQHNL
                     MRLGPIIRPETGLFFLGFILILTLDYHKIPGAIIIGILSISVLSLMLGLTSWQGLIAM
                     PPSLEPTFLQLDFSGLSSIVALKTIFTFFLITVFDATGSLIGLLSPTIFKHEKNYYKR
                     LTRSLTADAAASSLAGLMGSASTSPYIESATGIEAGGRTGLTALVIATGFILMLFLFP
                     LAKMIPSFAVGPALLYVACCMIKHITNMKLQDITETAPCMVIIMMVPFTASIADGIGL
                     GIILYTLLKLFTRQKPNPLIIVLSLVFIVFFLIN"
BASE COUNT        11292 a         7442 c         7010 g        10798 t
ORIGIN      
        1 ttcctgccaa ataatcaatt tgatataaat tattattaaa agccccccca gtatcagcca
       61 ggatgcccat tttagtcatt gtgcgtcctg atttgtcttg atactgaatc attagtaatt
      121 tccctaaacc aaactcagcc aaatcagctg caaaggtcac cttggggtta accgtaattt
      181 tgtattcagc gtccttacca taacctttaa ttccatcgac ttccttgaaa taccaaaatc
      241 gctcctgctg atagggagtt ttcgttcgat cataagcaat attattacta cgatggacat
      301 taaatatttt cttgccagca gtcggtccaa aatccgcgac aacggtacct tgcagtaaag
      361 ccgcctccaa atcatctcga cttaaataag ccaacttgct aaccggttga tttttaagag
      421 caccggataa tatcgcttgc ttaccatatc tgaaacggat tagtcctggt ttggcatttg
      481 cttcatccag agtcaaattc tgctcgtctt cgggcaaagc atacagagga tagggtttcg
      541 cggagcttgg tttggtactg gcaccagcca aatgcacaaa atatttagtc atcaatatct
      601 tatctttggg tatattttgt aataatggtt tattagcaga taattgtttg gcttggctta
      661 agtcaggata ccatcgaata aagtcgaaat gttgttttac aaaatccggg ttatttaatt
      721 ttttttggtt ttgacaaata aattccaagg tttttttcac tctgtctaaa gaaatcttaa
      781 tgacttttcc atcgtgaatc acttggggat cataattttt gcctttattt aagtaggcta
      841 aagtatgctt tgctgtactg cataattcag ttcccttaat gtcatatact ccattggcag
      901 gttgggatgg aacgaatttt ggcgcagccc ataatggaga aataaacagt actgctgaaa
      961 ataatgtcgc tattcgcatt tactctgttg tttccttttt atcttctgct tttcgagaca
     1021 atcctaattt ttcttttaaa cgggctacag catccggatc atattgcttt ggtgctttat
     1081 gtttgactac tacggatggt gacttggcgg attgcattgc cacattttgt gaggcatagg
     1141 tagagcttct gagaggatat attggcaaaa gaggctcagg atgagagtca aacgaattca
     1201 ttggcttaac gctggaaact gtacttggct gattatttac atgcgaatga ttagccgctt
     1261 ttgcattcac tagtttgcga gcatttttaa cactcttttc cacgcgtttt ttaattttca
     1321 tggaagcatg ttccgcttct tcctcagtaa cctcctctac tggatttccg tgcaaatcga
     1381 tacggacttc acgagctttc aggcaagcca gataatcaag acgacgggta aaaagaacaa
     1441 cagcttccct taatttactt ttagaaattc caacttgctc tgctttttct gcatgctgca
     1501 atatatcaga cataatacca atctttaatg gccgaatacg caaagaatta tcgaatgcct
     1561 cagggaaatt agccgcaagc catgataatg cgtcagatcg cgcacgcttg gattgatttt
     1621 tttgtgcttt attgatgaca gcggttcgtg ggtgcagcgc ctgctttctc attctggtac
     1681 ccttattgtg tttagtctat tggcatgaaa attatcaaaa tcatttgatt tgcaaaggtc
     1741 tgttgacctt gaatgtatga tagtcatatc ttgaagtcag atggtgtaaa ttatgctatt
     1801 agttactcga ggaatgagcg cacaatgtac aatgtttatt ggttgtttgc aagccttctt
     1861 tattatttcc attaagccga atgtcttttg gtttgtatcc aattagcaaa atacattatg
     1921 atatgtctat aattaactta agtcagatag aatagcgcag aggatgcttg atagattgct
     1981 aaataaagga accttatcgc tcaataatgt attaatggtg attacaaaat gaggataata
     2041 ctttcaattc ttgctatatt cataccgtgg ttggttttat tgattaaaga caatccaggc
     2101 ggagctgtcg ttgcgttgat tatgcaagcg acactaattg gatggccttt tgcttcaatc
     2161 tgggcatggc gggcagtcaa cccacttact ccggcgtctc aatccaaaca ataagtatcc
     2221 caatcctgta tttattcaac atataactaa atcaggactt acgaaaaatg ccaagtcgag
     2281 gcaaaaaatg tttttaatga gggagtttaa ataggctcaa tgaccgaatt aaaaattttt
     2341 ttataaaaag attggggttt ttcgtaaatc atgtaaataa ttcggagtga taaatgcgaa
     2401 cacgtgtcat tgttaatggt gccaatggaa aaatggggat actagcctgt gaaacattgg
     2461 aaaaccatga acagtttgaa gtagtagcca aactaagcag acaggataat cttgggcgaa
     2521 gtatactgga caccaaagca caaatagttg ttgatttaac aagagcagac tgtgtctatg
     2581 aaaactcttt aaccattatt aatcacggcg ctcgaccggt tattggaaca tcggggttgg
     2641 ttgaaacaca aatcgatgag ctcaccaaac tgtgtgaaat aaaacaaata ggtggaatta
     2701 ttgcacccaa tttttctctc ggcgccattt taatgatgat gctcgccacg aaagcttctg
     2761 aatatttttc agaggttgaa atcattgaag gccatcatca acaaaaattg gatgcaccat
     2821 cagggactgc tttaaaaact gcagaaatga tagctgcggc gcgcaaaaaa cccaaaaaca
     2881 aacttccatt aaaagaatta acccctgggg cccgaggcgg ctctcatcat gatattaata
     2941 ttcattccct tagactgcct ggattgcttg ctcggcaaga agtcctcttt ggcaatatag
     3001 gcgagacact ctccattaca cataacagta tagatcgccg ctgttttatg ccgggaattg
     3061 tgctggcctg tcaaaaagta ttgaatttaa ctaatctggt ttatggtctg gaacatttgc
     3121 tttaaatttc aaaaaccagg ttgacccgta ttactgtcgg gcatacgggt ctccttttcg
     3181 catttcaccc cttgatatgg aacgtttccc aactcaattt tttaaacatt acatataaac
     3241 caatccactg ttttctcttt tggaactctc aaacaaagaa gaccattcca aaatcgcttc
     3301 ttctgccagt gtttgtaaag tggtttctga tgacaggggc tgtaataatt ttctaatacg
     3361 atcatagact gaattggttg ctgtagttct tgatgagtcg ctttctggcc gatttaaaac
     3421 ggctgcaaat atttttccaa tacgaagaac attatcattg cccgagtttt catcatgcat
     3481 tacttcacga atattgagta ttccagtagg tgttgagctt acatctttat cagagaacca
     3541 acgtaatttc caggaatctt tgcttgattt ttgaaatctg cgctcctcat tgattaaagc
     3601 gcttagcaca tcataaagca ttgtacacat tttttcgccc agctgtttgg caattaacaa
     3661 gcaatgcaat tcgttttctg gtagaaaaaa ggaacgcaaa tccttgctaa tattctttac
     3721 ttcattatca gtggctaatc ggtcatcgta ggctaatgct ttctcagtgc ccaagcgctc
     3781 gttaatggcc tcagcgatat cgtttggcca gtaccaatca ggcgttttga ttccctctga
     3841 acctggcgca ttttgccctg caagctcatg ttgatgtctg gagatataaa gatctacaac
     3901 aatattgatg aaattgactc gttcttgttt ttctttggga attccaaatt taggccaatt
     3961 tccatatttt gctttataca aaatcatcgc atcagtatgc aataactcta ctgcttttct
     4021 gtctttacca ctgacacagg agccatagct atagccgccc atgtttagaa taatcagttc
     4081 ttccaaagag ctaagaaata attccctgcc atcgtaatcc caaaatgttg cagagcctaa
     4141 aggcgactct aaaacggcct tataatcatc gattagagcc tgcaatccag gcttggaaga
     4201 cgcatatttc tgcgctgctt ttaataaaaa ctcgctgtct gtatccgttg cctgagtata
     4261 ataataacgc ttggcaatgt taaaaggatg attatgctgg aatatttcat gcctgcgttt
     4321 gctccgggtg accgcttctc gggcaatttt atacaactcc agatccggag gtaattccgg
     4381 taaaaagtct gtaaccaccg ttggaatata atctactgca tggatagggc taattaatgt
     4441 ttgcaataac aaaggctgct gaggtttggc aaattccatc accttaacca ggttggagtc
     4501 aacatgacgc tgctggacat cctcaggaaa ttttaacgaa tcacgactgg caacatggct
     4561 tgagcgatac ctcttgtcat aaaataaagt ttctttaccc tcatcatcga ttaaatataa
     4621 actgtgcgca ccataattgg ctggagcagg caaagttcgg tgccggcttg ataaataaga
     4681 tacgacatcc tcaacttttt ctgcattttt gagagcatat tccaaaaagt attgttgttt
     4741 ctcggataaa acccaatacc actgtggaag cttaggcact aggctcaaag tcgatgcgta
     4801 ttgatcattc ctggcctgct ccaccataaa ttttttaaac tcttttaagc tggatttaat
     4861 ctcatgtgga gtagcagaca agacgcgaat catggcttgc tgagcagggc ttaattcatt
     4921 aaaccaggaa ggataaggcg gtgagttggt atgtatttga tttaattccg tggtcaaatt
     4981 cagtgacctt tttgcaatat ctccccaatt gccaaaaaat gtattaaagt gctgtagcgc
     5041 ttttttagga tcaacagaac ttaaattcag attacaataa taagcttgtt gatagggcgg
     5101 cagattttta aaccaggcag gtgttttagg aaaactatat tcttttaatt gttttaattc
     5161 agttaaccat tgttcataaa cgggagaaag ggattcatca tattgcaaaa catactttgt
     5221 ctcactaccc atttcaatag gcattatggt agcgatatcg ctacgacccc gactcattaa
     5281 gacatactgc tctgcttcat taaggctagc tatcgcctct ttgactgcct tacctttagg
     5341 ccatttccac gcgattgcaa tagcctcaac caatttggcc gtaaatcgtt cgagctcaag
     5401 atgcaactct tctaatgtca gtttttccag gtcacatgaa gccttgcatt ctgcggcata
     5461 agtacttaag gttgcaaccg tcagagaagt atgttttgga tcgagggggt aagcttttaa
     5521 gataaatgaa aaggcatcat gaaggctgtc tatccggcga gcgatgcttt tacgataatc
     5581 tggctgctct ttgaaatact ctaatttgta accaggtaga agtcgctcta gtgtatcaaa
     5641 tacctgagga gataatattg tattttccaa aacacgtaaa attatacctt ttcccatagc
     5701 ttaattccct tactgcctta atttcctcat tattgtataa ataattcaaa gtgacaatgt
     5761 tttgaattaa caattgaatt atatatttac cctaagatat caggaagtta attgccttaa
     5821 tagcattact tcatgctaaa ctagtttttt tggaggaata gtaaaaggat agctatgaaa
     5881 agtatagagg attttacagg tttttcagaa tccgggattg atgatgccat tcaaaatgct
     5941 ttaaaaaaag caggtaatcc aactcatttt gaagtcgttg aaacactagg ttcacaagat
     6001 agccaatcag atcgacgata tcgggtaacg cttaaaacac tgagtgatta atcattaagt
     6061 aacggcaatc attggctaga aataaataac aaaaatttaa tgtcagttct gtttctataa
     6121 ctattatcta gctgctgcct ttcaatacaa ggagtttaaa aatgggatac aatgtgcctc
     6181 actatattgg tggacaactt attaatgaaa cggatactga agctcaagcc atttataatc
     6241 ctgctctggg tgaaattatt ggccaagtcc atttcgcgtc aaaggcaacc tgtgatagag
     6301 ctgtggcaat tgccaaagcg gcaggaaatg aatggtcgca aacccctgct ataaaaagag
     6361 cgcgagtttt attccgattt agagagctgc tagaaaaata ccaaatggat ctggcacgaa
     6421 tagtcactcg tgagcacggc aaaacgctag atgatgcgaa aggatcggtg gccagaggca
     6481 ttgaggtagt ggagctgcac tgcgggctcg tcaacgagct aaagggagat ttttctgctg
     6541 aagtggccag ccatattgat tgccatactt ttcgacaacc cttgggagta tgtgccggag
     6601 tatctccatt taactttcct gtcatggtac ccatttggat gatgattcct gccattgctt
     6661 gtggaaatac ctttattctt aaaccctccg agcaagatcc ctctgcgcct gtccgtttat
     6721 tggagttgtt aagtgatgcc ggtttgcctc cgggcgttgc caattgtatc cagggtaata
     6781 aaacgactgt cgagcattta ctggctcatc cggatatagc tgcctttact gcggtcgcat
     6841 ccactcctgt tgccgaatac atctatacca ctgcagcagc ctatggaaag cgtgcacata
     6901 cttttggcgg ggctaaaaat cactgtgtgg ttatgcctga tgccgattta gaccaggcag
     6961 ctaatgctat cgtcggagca gcctatggtt cggcaggaga acgttgcatg gctttatctg
     7021 tcgttgtaac ggttggtcaa cataccgctg atgccttgat caataaaatt tcccccctca
     7081 ttcgagccat tcgcgtagac gccggagatg ttgaaggcac tgatatgggt cctcttgtca
     7141 gccaggctca tcgacaaaga gttttggccg ctattgaaaa aggggttaat gaaggtgccc
     7201 gcttgattat cgatggcaga acctttcaac atcctcaata cccacagggt ttttacctgg
     7261 gaccctgctt gtttgatgaa gtaaccgaag aaatgtctgt ctatcaaaat gaaatttttg
     7321 gacctgtgct ggttatcgtg cgagtcaacc attttgaaga ggctcttgct ttagtcaata
     7381 aacatcaata cggaaatggc actgctattt tcactcggga tggctttaca gcacgtgagt
     7441 atagccagcg agttcaagca ggcctggtcg gcattaatat tccaataccc gttcccattg
     7501 ccaaccatcc tttcggaggc tggaagcgct ctgtgtttgg agataccaac atgcacggag
     7561 aggaaagtat acatttctat actcgacgca aaacggtcac cagcaaatgg ccaataaccg
     7621 aattaaagga taacagcttt gccatgccca ctcatgatta atcatgctta ttacggagaa
     7681 atatatggct aatattggat ttgtgggatt gggtcatatg ggttttccaa tggccattaa
     7741 tcttttgaaa gccggtcata gtgtcatggg ctatgatttg caacaatcag cacttgaaca
     7801 ttttgctgaa caaggaggag ctattgctca taacttgcag gaattggcaa aaggcaaaga
     7861 tgtcattgtt actatgttac aaaccggaca gcaagtttta catgtatgcc ttggtgtaga
     7921 tggtatattc agtcagttaa atgctggctc attgtacatc gattgctcta caatcgatgt
     7981 aaaaagctct cgagaaaccc atcaaatagc gaaagaaaat catcttctgg tcgtcgatgc
     8041 tcccgtctct ggcggcgtag cgggggccgc tgcagccacg ttaacattca tggtcggtgg
     8101 tgaagcggag gcttataatg cagcgcatcc cattctctct gccatgggga aaaaaatcat
     8161 ccatacaggc ggtgcaggca gtgggcaagc ggccaaaatt tgcaataaca tgatattggg
     8221 taccacgatg atcgccatat cagaagcgtt cattctggcg aaagaactag ggctgtctga
     8281 tggaaaatta tttgaggtgg taaacaattc atcgggtcaa tgttgggcta tgagcaaata
     8341 cgtccctgta ccaggaatat tggaaaatgt tcctgcaaat aatgattata agccagggtt
     8401 tgcagcaaac atgatgttga aggatttgtt attgagtcaa aatactgcga aatcagtaca
     8461 cattgaaaca ccccttggcg ccaaaacaac tgaaatttat caacaatttg tcgatcaggg
     8521 acttggtgat atcgattttt cagccattat taaacacatt ggcaaaacac aagaggtata
     8581 gcatgcttcc tgttcatcta aggcaccctc atttaacacc tacagaagaa tctttatctg
     8641 aattttggtc tgaaattgcc gcgcaaacca ttgactggat acaaccttgg agtattaccc
     8701 tgcagggagg attacacaaa ggcgatgtga aatggtttca aggcggcttg ctcaatgtta
     8761 gcgctaattg tctggatagg catttacccc ataaagcgaa tcaaacggcc atcatttggg
     8821 aaggtgatga tgaaaaccaa aataaaaccc tgacttttgc gcagctttat tccgaagtat
     8881 gcaaaatgag taatgtgtta aaaagcctaa atgttcgcaa aggggatacg gtaggcattt
     8941 atttgcccat gattcctgaa gcggccattg ccatgctggc ttgtgctcgt ataggagcta
     9001 ttcataccgt tgtgtttgcc gggttttcgg cccatgcatt gcagcaacga ttaatagcct
     9061 cttcctgtaa atgtttaatc actgcggatg cgtttcaacg cggtggaaaa actatccctt
     9121 taaagaaaca ggctgatgaa gccagcgtgg acttaaatat caccaagctg gttgttaaaa
     9181 attcaaatga cccaacggca ctaaataaga acaaagaaca ttggtggcat gagctaaaac
     9241 aaaccgtatc ggatcaatgc atgccggaac caatgaacgc cgaagatcca ctgtttattc
     9301 tctatacttc aggaagcacg gggcagccga aaggggtggt acataccaca ggtggctatt
     9361 tagtacaggc cgcttataca catcaactga tatttgcttg tcaggataat gaagtatttt
     9421 ggtgtactgc tgatgtcggt tggattacag ggcatagtta tgtcgtctat ggacccctct
     9481 gcaatggcac taccacattg atgtttgaag gaatacctac gtggcctgac tcagcaagaa
     9541 attggcgcat cattgataag caccaagtga atgtgttcta cacagctccc acggccatcc
     9601 gctccctgat gagagctggc gatcaatggc taaactcaag ctcaagaagt tctcttcgtt
     9661 tactgggttc agtgggtgag ccaattaacc cggaagcatg gaattggtat caccaaaaaa
     9721 ttggacaagg gaaatgcccg atagtggata catggtggca aactgaaact ggagccatta
     9781 tgattagtcc cagagccagt gatgaagtga tcaaaccagg ctctgctcgt aaacctattc
     9841 cggggatcgt accactcctg ttaaatgagc aaggacatga aattaatggc gctggagaag
     9901 gccttttggc tatcaaatac ccatggcctt ctatggcaag aaccattgca ggagatcacc
     9961 aaagatactg taacacttat ttatccaacg gctattacat cactggtgat ggggccaaac
    10021 gagatgagga tggagattat tggattacag gccgaattga tgacgtactt aatgtttccg
    10081 gccatcgatt gggtactgct gaaattgaat cagcattagt cagccatcct aaggttgctg
    10141 aagctggcgt tgtaggtatt cctcatgact taaagggtca aggaatattt gcctatgtca
    10201 ttctgaagca gggaaataag ccggatgcca agctccaaac tgaattgatg gagcgagtca
    10261 aagatgaaat cagtgctatc gccaaaccag atgtcattca gtttgtaaat gatttgccaa
    10321 aaacccgttc aggtaaaatt atgcgccgaa tactaagaaa aattgcttgt aaagaagtgt
    10381 cgcacattga tgagctgggt gatttaacaa ctcttgctaa ccctcagatc gttgaggagc
    10441 tcatgaaaaa tattttgaag cagtaaaaag aaccgtcgag cttcacaaag tttattctga
    10501 tagcaaacaa ataaactatg tgaagcctga caaccagttc aaataatatc aaatcaattt
    10561 catcaggctc tgacagcgag cggttgcatt gacaatgaaa aatctttgac aattttttca
    10621 gactcggtgg gcacaggaaa tagtcgccat cttcccgcgt ataaattggc tccgcccaaa
    10681 gtgcataaag tgactaaagc actcgctaaa tcagcaaaca cttgtttcca accacgatgt
    10741 acttctaaaa caggtatgac tgcattaatg gcacggacgc aatcttgatg aaaatccttg
    10801 catttcacat tcttgggcag ctgagagttt ttgaagcgct cttcggccct taataaatta
    10861 ttgtaaaatg ttctcgctat gggtgccgcg cgtttataat tttcatcgcc aaccgctttg
    10921 gtttccagcg ttttaaccat actttcaata gttttcaagt ggtcggaaaa tttaatttcg
    10981 gcaagaatat tcaaggtttc tgttgttgca gcgatttggt cagtaatacg cttactcact
    11041 gcatcagctt gcctgttaat ttcgtgaact ttatctgtca gagccatttt gatatcacta
    11101 tgatactcac cagataaaag ttgaagctgt tcctgagcct taagcaaatc gggtttgttg
    11161 cccaccaaat ttcgaaccga ttgaattaaa tcctgttttc tggcaatcac ttcttcaact
    11221 gtttggcatc tgttaaaact tatcggaaat tccctgattt gtttttcata gctttttact
    11281 acttcttcag catttatttt aatgatcaat tgatcagctg tttcatttat ttcttgtttt
    11341 ttcgcttgca gtgctcttac tatggatggt tgcaagtcat tacaatccag aactttttca
    11401 gcacttaaca aagcttgatt aggtttaacc agattatcaa gttgttgcaa taagttattg
    11461 cgctgccatt ctactgcgga agcggagctt atatcatcaa agcgaattac aaaattatca
    11521 attctttttt caacagaatc gataaccttt tgcgcctcat caagaagctg tttttgcaat
    11581 acttcagccc gcaaattaat cttttcaacc tgagcataat agaccatttt tatttttgga
    11641 ttggcgatga attgttcgcc caaaagatct tccgctttga ccagtgtgtg aagctttttg
    11701 ttcaacagaa tacgttggga ttcaatagct actatggtat ccgcatcatt aaagtcgatt
    11761 gggaaatttt ttatttgcct gacatgctca tctatatccg tttcatactt tctgagaaaa
    11821 tctgaacgtc gattagctgc ggcatctatc attcggagct tacgctctaa agccctctgg
    11881 acttccggat gtccatcagg taaatccagt atttgcaaca cattttgcaa ttcgccagaa
    11941 tgaactattt tcttaagttg gcctctgagc tcttctgcat taagattaac gtgtgctacg
    12001 gttttaaatc cctcaaaact aaatggaaaa gaattgattc gctgaacaat atctgaaatc
    12061 actcttttag catcgtcgac tcgttgttgc cttaatacat tatcaatttc tggaattttt
    12121 atcacaggtt gtggcttgga taaggactca aaccatgatt tgaattcgcc tgaatctttt
    12181 aaagcgattt tttttgcgta ttcttctaaa gatatgccaa atagaagatt aattttttga
    12241 gatgcaaaga caaataaagc ttttgccact tctggattaa ttcggcttaa tctgaacaaa
    12301 ttttcaaaga attcatccgg atctttaaac gttgcgctgg caagaacgac atcaaaaaac
    12361 tcctcatcaa ttggcgatgt gtctttgctt tttaagtact caatcaataa atccggtaaa
    12421 gtctctagtg cgagatagtt agcaatcgct tttgcaaaaa gagcttttgg cggaaataat
    12481 gcctctattg attttttgga gaaaacattg gctataaaca atgctcgtaa ccctgatgct
    12541 cttgttaaac tagaggatgc tccaccaaag taaaagttaa ttaacccgga ttcaatttct
    12601 tgtttcgctt tttcaggcat gtaagctaac tgattgattt tttgctgtat tgcagacttc
    12661 actattaaat ggagaaaagc ctctccatta aaatttatgc ctgctttggg acttactttg
    12721 ttttcttcga ctgggtttga caaggctaac tctgtcaaaa cctcgcctaa taattcttga
    12781 gatgtttctt cacgaagaac cagttcaagc aaatccattg cagggatatg ggttaaaaat
    12841 aattttagga attctttttt agaaccatcc gcattggcga tgcaagtagt taatgttttc
    12901 ttttcctcca ctgacaattt taggaaaagt ttttcaagat aagggatttg tttatgcaaa
    12961 ctaatgcaag aagaaataat tttgttgtta gttagaagat tcatttcctc caaagatatc
    13021 agccttttct gcagaccaga attagaaaaa acatacctcc cctcctcgtt ggaaagagtc
    13081 aataattttt ttgtcaaact ggctctatgg atactggtat taaaaaggca aaatcggcag
    13141 tttcgtttgt tgagatcgtc taatttgtaa gtttctgttt tagaattatc gtgtggattg
    13201 attaaaacaa aatcataatt tccagagcct ttaggaataa ttttgtctat tctcaaagca
    13261 tgacggccat gaaattttcc aaagctatcg acttttccat aagacatact aatataaaca
    13321 ggttcatcag gattcattaa tttcaatttg atgatatcgt caagctttga atatggaatg
    13381 tcttccgctt gaataccaaa gaatttgcct aaaaaagcag tggaagtaaa accggcaatt
    13441 ctgtccggta tatcatgagc aacaacgctt gccaaaggat cagtattact ccaatctccc
    13501 gcataataat aggaaaccaa tcgttccaaa atttttattg ccagtgaatt actttttacc
    13561 cctccgtatt gattatcaat ttcctttagt ctttccgggc taatctcaaa cacatcttca
    13621 ttgttgagtt catccacgta atgggtgtac ttcccagtca ttttgttctt ttgcaaatta
    13681 tcttttagtg cctcatggcg tttgattctt acgataactt tcccatcttc cgtttgggta
    13741 aacaatgatt taattaattg ctccccatcc gccccaagat ttaaaatgca atccagagat
    13801 gacaaaagat agcaactacc tccaatacct tgtcggatgg taatagggcc ttgagttggg
    13861 aacaaaggtg tcattgtggt cctcttaaaa tcatagcgta cagaaagagg gcattataac
    13921 agcaaaatat tggataatca ttaaaaaaaa tggtctgtaa tcaatcggaa aaccaaagcc
    13981 ctaaaagcta attccactca taccaacggt caagcacgga aatcaactcc tcaacaccat
    14041 cctttttcac agaagaaaac gcttgcacag aaaccaaatg ctctgccagc tcataatact
    14101 gccgaacttt aagtacggca tttttgactt cactacgact taacttatct gcttttgtca
    14161 gtaaaatatg cactgggagc tctctgtgca aagcccaatt cactaatatt tgatccaaat
    14221 ctttcaatgg atggcgtaca tccatcaaca aaattaatcc tctcaaacat tgccttgctt
    14281 ccagatagtg ggctaaattc ttttgccatt ccaattttac ttccattgcc accttggcat
    14341 aaccataacc tggtaaatca acaaggcgcc tttgctcatc aagagaaaag agattgatca
    14401 gttgagtgcg gcctggtgtt ttgctggttc ttgccaaatt tttattatta gtcagacaat
    14461 ttaaggcact ggattttcca gcgttggaac ggccagcaaa cgctacctca tacccggaat
    14521 cttcaggtaa ttgattgaca cgggctgcac tttttaaaaa tactgcttta gaatataaat
    14581 tgattggcat aagatatcat tttagttttg ggattttgac ccaggcagtg taccattaat
    14641 tgactaaatt attatgagaa atccatgaaa aaattagcac tcgtattaat cctgtgctct
    14701 tcatcagtga tttttgccca ggaaaacccg caagaggctg gacaaaataa gtcaacagtc
    14761 tgtaccgcct gtcatggtcc gcaaggtatt agtacgaatc ccgaatggcc caatcttgcc
    14821 ggccagcatg aaaaatattt tgtgaaacaa ttgaaagata taaaagaagg taagtcgcgc
    14881 tctgcaccca ctatgactgc aatagttgct aatctgaatg agcaggatat ggatgacttg
    14941 gcagcctatt atgctaaaat gcccgttgct gaaggttcaa cccctgaaaa atatttaaaa
    15001 agaggcgaac aactttacag aggtggtgat ttaaacaaac acatcgcggc ctgtatagcc
    15061 tgccatggtc ctaaaggaag tggaaacgct caagcaggct ttcccctgct ttctggtcag
    15121 catgcagctt atactataat gcaactgcaa gcatttaaag acgggaagcg aacgaatgac
    15181 ttaaatcaaa ttatgcaaga tatcagtagt aaaatgagcc cagaagatat ggaagccgtt
    15241 gcttattata ttcaagggtt atattgagga attgactatg tttaaaaaat taattgccct
    15301 attatttctt ataccaatga ctgctttggc aactcaattt attgaaggca aagattatca
    15361 aaccgttgcc agcgcgcaat tgtctaccaa taaagataaa acacccctga ttaccgaatt
    15421 ttttagctat ggctgtccat ggtgttataa aattgacgcc ccactaaatg attgggcaac
    15481 cagaatgggc aaaggtgctt atcttgagcg cattccagtt gtttttaaac caaactggga
    15541 tttgtatgcc aaagcctatt acacagcaaa aaccctggct atgtccgata aaatgaatgc
    15601 catattgttc aaagcaattc aggaagataa aaatccactg gcaacgaaac aatcgatggt
    15661 tgattttttt gttgctcatg gcgtagacag agaaattgcc aagagcgctt ttgaaaattc
    15721 gccaacaatc gatatgcgcg ttaacagcgg catgtctttg atggctcatt atcaaattaa
    15781 cgccgtacca gcctttgtcg tcaataacaa gtacaaaact gacttacaaa tggcagggag
    15841 cgaagagcgt ttatttgaaa ttttgaatta cctggtaaga aaatcagcct aattaactca
    15901 agctataaag gatattccta atcaggtcag taccctcatg cttcaatatt ctctaaaagc
    15961 cagatttggt tgggatgggg gctgatctct tacgtatatt cctcgccaag gtaattgacc
    16021 acaggccagg tcattttgac ctggctttct tgctaagccc ccttgcccgg gttttccaga
    16081 ctcaaaatac cggtattgaa atcataagca aaaatctcgg catatcgtcc ccagtcaatc
    16141 attgttttca aaacacgatc tgcttctttc tcgcttaaat aatcctccag cttactcaaa
    16201 aagcgctctt ccgagacgcg atgccccgct ttctcatcca gtacacgtct tatataacga
    16261 gccagaggga ctttttccaa taatctttgc gcgaacaatt gcttacgctc ttgcaaatca
    16321 gcttctgaaa attgctttcc taattcactt aactgaatat ctcccgcaga caccttggca
    16381 aaaccaagta tctccagagt ttcaagaatt gggaacagat catcaatatt catcatgagc
    16441 tcgtctgcca gctcaggtaa atcaatacgt tcttcaaaag acttcatagt ttctatcaga
    16501 ccggataatt cagagggctc gacatcgggt aagcgatagc ctaaacctat ttgacgctcc
    16561 ctttgcgctc gcttggcttt ttctttgggt ccagtagtca ttaaagtata aatcttatca
    16621 accaaggcaa gatattccgg gctttccggg tcacggggtt gcggaagagt cactggtaat
    16681 tcagcacgaa tatagccggg atcattacca aaaatcacta tcctgtcagc caaagtcgcc
    16741 gcttcttcta tattgtgtgt tactaataaa ataccattgg tattcgtttt cttttctttc
    16801 cataactcca ataaatctga tttgaggttt tctgcagtaa gtacatcaag cgcagaaaag
    16861 ggttcatcca ttaacaacac atccggatta ataaccaaag cgcgagcaaa accaacccgt
    16921 tgtcgcatcc caccagataa ctccttggga aaagccgatt cgaaaccatc caaacctata
    16981 atatctatcg cttcaatagc cctgtgcctt ctttcctcgc ggctgattcc ttgtgcttcc
    17041 agaccaagct ccacattttc cagaactgta agccagggca ttaaagcaaa cgactgaaat
    17101 accatagcaa tgccttctac cggcctagtt acaggtttac cgcggtaagt cactgttcca
    17161 ctggaaggag cgataagacc agcaataatc ctgagtaaag tggatttccc tgaaccagat
    17221 ttacctaaca aagcaactat ttcaccttcc tgtaatttga aattaacatc ttcaagtaca
    17281 agaaggtgct gggaaggtgc ttttttaaaa gatttgctta aattttctat gttaataatt
    17341 gtttccggca taggagtctc aattaaaatt aaatcgttct tcggctaatc gataaagcgg
    17401 gcgccaaata agatggttga aagctaatac atagacgcac atcattgctg ttcctaatgc
    17461 aatttgcggg aagtctcccg ctgtcgtact agcctggata tactccccca gacctgtggc
    17521 cttaagagta atatttcccc agctcacgta ttcagcgacg atactggcat tccatgcacc
    17581 gcctgctgct gtaatagctc cggtaatgta aaaaggaaaa atacctggca aagccaatct
    17641 tttccaccat atccaaccct ttaacccaaa attatcagct gccagataaa gatcgcgggg
    17701 tatactcgag gctcctgcga tcacattaaa taaaatatac cattgtgtgc ccaggatcat
    17761 gagcggagtt acccagattt ccacgctcaa attaaatttg acaatagcaa ttacaaataa
    17821 aggataaaat aggttcgctg gaaacgctgc cacaaattga ataatcggtt gtattttttg
    17881 agccagcctt ggccttaatc ctatccacac tccaacggga atccatagca tagagcttag
    17941 caaaattaat ataatgaccc gagcccctgt tgctgcgccc aataaaaaga catgaaatat
    18001 atcactcacc ttcaattcag cgagaataaa tttcaacaaa aaccacccgc cagtaataac
    18061 ggctatcaga accaacgctg tccacaatcg gtccaaacgc ttttgccttt taaaatcaat
    18121 ttccttaaca gccttcacct caccaatcct cagccaacga gcatttacaa aactattggt
    18181 aaagaccgcg agccactccg tcactctctt catcaaacga ctgctgcgta tcaagtcaat
    18241 taaccaggat tgatattctg actcatcggg agactgttcc attttaaatt tttcagacca
    18301 ggcaataagt ggcctgaaaa gtatttgatc atacaaaaaa ataacgataa ccatggtcag
    18361 aatggcatat cccacagcat gcaaatccct ctgggctatc gccaatgcga tataagaacc
    18421 tacaccgggt aatctgatat tttggtgcgc caccgaaata gcttctgaca aaaccacaaa
    18481 aaaccagctt gccgacattg aaaccatcat attccataac agtccagaca tggaaaaagg
    18541 aacttctacc ttccaaaagc gctgccaggc tgacaacctg aacatggcag agacttcaat
    18601 caaatcatga ggaactgttt ttaaagattg ataaaatcca aaagtcatat tccacacttg
    18661 ggcgctaaaa atagcaaaaa ttgaggcgca ttctggacct aacaagctat tgggaaataa
    18721 atgaataaac ccggtgacag taatagataa aaaactcaga acaggaatcg attgtaaaat
    18781 atcaatcgcc ggtataacaa tttgctcagc cctgcgattt ttggcagcta aagcacctac
    18841 aataaaagta aacagaattg aaaaaatcaa tgcgataaac atgcgtaaaa ctgttcgtaa
    18901 cgcataaaag ggaaggttag aaggctccaa agaaatggga atttgatccc ctaattgata
    18961 cggggtagcc atctgtgaac cggcccaacc caggaaaaat aacacagaaa aagtcaatat
    19021 aagcaatagc aaatcccatc gattaataaa tcgactgaat ttatcaggat tagcaaaata
    19081 aaaccgacta ttgcccacta taccctctct taaaattgac gttacaagtt tcccttaatc
    19141 taatccttaa atctaaaaaa tatttgtccg ttcctgaatc aggatttacg aaaaacccca
    19201 aggtcaaggc aaaaaagttt ttaatgaggg agtttagata aactaaacga ccgaattaaa
    19261 aactttttta acaaagaaat tggggttttt cggaagttct gtgaatataa aataccataa
    19321 atacaacgtc atcgatacca gacaaaccaa cacattcaac cgtatttaca aataattttg
    19381 attaaatttt gaactttttt gcgattcatg tgtctaatat agtgagcatt ctcccccgag
    19441 tgctctaccc cgctttcccc ctctttatgc ggggtagtat ccggattgca ccaaatccgg
    19501 atatataatt ctccccggga tcttagccgg ggattttttt gtaatgaaaa aatgactaaa
    19561 ctggcagtat tgatataatc gatgagaatt tattatgaac aaaacaatca ctattgcctt
    19621 gagtttactt ctaagtattg cctcaaaact aacttatgca gggccagata gagcacaatt
    19681 ggcagtttgg gctaatgaag cgattattgc cacttacact tttgattata aaaattacat
    19741 gcaacaacaa aaggaaatag ccaaatattt ttcagctgat ggctggatag cctatagcaa
    19801 agcgcttaat caatcaaaac tgcctgaagt agtacaaaaa aatgcctatt tcgtcaatgc
    19861 tgttgccaca gaaccaccca agctgatcac cttggatccg acacactggc aagctatcat
    19921 gcccatactg gtcgtatata aaaaccccca atacgagcaa aaacaaaatt taaaagttat
    19981 attagggttt accgttgcat ctcccggaca aggtgtaaga ggtttcagcg taaccagcct
    20041 gcaatccacc cccatcagcc cgccatgcca atgtaaaatt gaagagacgc ctgggaacac
    20101 taaacaagga gacgccaaac aataatacca atatgcagca aaattaatga gctgattaga
    20161 attaaaataa aagttagttt tttaggtatg gtaacatcta aagcagattg ttcaggacga
    20221 ttgggattat agtagacgtc aatttctgag ttatctctgt aagctattgc cactttatat
    20281 gcaatacttc gggaatattt actattgggt gtattaaatg ttgtatccaa aaacaaatat
    20341 tcccctgtca aatccttctc ataaacttca tagatatatt ctattctagg ccaaacacta
    20401 tgccctactc ttgtccactc acagcgcgtg acatgcccct tcgcttttaa ccaggatctt
    20461 gcttcaacca aatctcttct ttttttccaa aaataccaaa aaagaattaa aagaaaacac
    20521 agccatccta aatcaaggat ccatctccaa attatcggcc acaccatatt ctttcccata
    20581 aatttatatt atatgctatt tccagggaga ggatcatgca aaaaaaatca aattggaaac
    20641 ctcgcaaaat taatcccaac aggtataatc acatccaata agtgataaaa aaaggaaatg
    20701 caatgactgc taaaactcct cttcacacta cgcacttagc atgtggtgcc aaaatggtgg
    20761 atttccatgg ttgggatatg cctcttcatt atggatctca gttaaacgaa catcatgctg
    20821 tcagaaacga tgctggcatg tttgatgtct cacatatgac catcgttgat attttaggcg
    20881 ccggtggccg tcaatttctg cgcaaactgc taaccaacga tgtcgatcaa attactcata
    20941 atggaaaagc cctatacagc tgtatgtgta atgaacacgg cggaattata gatgacctga
    21001 ttgtatatca acgcgcttcc gataattacc gagtggtact taattcggca accaggcaaa
    21061 atgatgtggc ctggattcgc gcaaaaagtg aaggatttgc tgttggctta caagaaaggc
    21121 gcgaattatc aatgctcgct gtacaaggtc caaatgccat agcgaaaaca ttgagtattc
    21181 ttgcacccgc ccatgtagat gccgtttcaa ctcttacccc tttcgaatgc gttgatgtag
    21241 atcactggtt ttttgctcgc accggctata cgggggaaga tggccttgaa atcattgtcc
    21301 ctaatgaatt tattactcaa ctctggaatg atctcctgaa tgctggagta acgccatgtg
    21361 gattaggggc cagagacacc ctgagattag aggcaggaat gcttctttac ggccaggata
    21421 tggatgaaac aacaacccca ctggaatctg gtttggcctg gacagtaaag tgggagccgg
    21481 aagatagagg atttatcgga atgggcgctt tggtttctca aaagcaacaa ggaattaaac
    21541 gtaaaatggt cggtttaacc ttgttggaca aaggcattat gcgtcatggc caaaaggtta
    21601 ttattgaagg ctgtcccgat ggaattatca caagcggcag ttattctcct accctgcaac
    21661 aatctatagc tttggcacga gttcccgtgg aaacagggga acaagtactt gttgacatac
    21721 gcggaaagtt aatcccggca aaagtaggga agcctcgctt tatcaaacaa ggaaaacctg
    21781 tttaaatttt agtaatatta atattaagga tatacaaaat gaatgatttg aaattcacaa
    21841 taacccatga atggctgaga gaagatgagg aagaggtcac agtaggaatt acagatcatg
    21901 cgcaagaatt attgggggat atggtatttg tagaactacc cgaaataggt gatgaagtga
    21961 gtgcaggcca agaattaggc gtggtggagt cagtgaaagc agcttctgat ttttatgctc
    22021 ccattagtgg tgttgtaacg gcggttaacg aagccgttgg taaaaaccct gccctggtaa
    22081 accacgatcc ctaccatgaa ggttggctcg tgaagctaaa gcccagccat cctgatgaaa
    22141 taaaaagcct gttgagtgat gagcaatatc aaaatgagat agctgaggaa aattaattat
    22201 gccttatatt ccacacacgc ccaatgatac caaagagatg ctagcggcaa ttggtgctca
    22261 agacattcag gatttatttg atgaaatacc tgcttcattg caatatgcag gatttcaaaa
    22321 tatccctgct ggcattaacg aaatggaaat gttgaaagaa gcccagaatc aagcccaaaa
    22381 gaatcgcaac ggtatatgtt ttattggtgc tggatgttat gagcatcata ttccagcagc
    22441 cgtgtgggat attgcatctc gcggtgaatt tttaactgcc tacacaccct atcaagctga
    22501 ggccagccag ggaactttgc aattattgta tgaatatcaa accatgatct gtgagttgac
    22561 cggcatggaa gtatccaatg cgtcaatgta tgacggagca actgcgttag ctgaagcagt
    22621 actaatggca gtccgcctta ataaacacag taaaaccaac agggtactga ttgcaggaac
    22681 tgttcatcct ttttatcgcg aaaccattga aacgatagta cgtaatcagc atattgaagt
    22741 catcaccctc ccttttgatg aacagcaagg aatcactgat cttggctctc tcaatcaata
    22801 taccggagaa gatattacag ctttggtcat agcccaaccg aatttctttg gttgcctcga
    22861 acaagtcgat aaaatgactt cctgggcaca tcataacaaa acaatcagtg tagcttgtgt
    22921 taacccgact tcattggctt tattaaaacc acccggctca tggggagaac atggggtaga
    22981 catcgtatgc ggtgaaggac aacctttagg ttcacctatg gcatcaggag gcccttattt
    23041 tggttttctg agcacccgta tggcccatgt cagacaaatg cccggaagaa taattggccg
    23101 cacggtagac aaggatggaa aaaccggttt tagtttaact ctccaggcaa gagaacagca
    23161 tattcggcgt gctaaagcca cttcaaacat atgcaccaac caaggtttac ttgtaacagc
    23221 agcaactatc tatatgagtc ttttagggcc ggaaggtcta agccaagtgg caactcaatg
    23281 ccatcaaaac actcatgaat tgattagtgc cttaacacaa atcgaggggg tagaactggc
    23341 attcaaagct ccgtttttcc atgaagcctt gattaaacta aaccaaccag ttcagtccgt
    23401 attgcaacaa ctggccgacg ctggaattgc tgggggttac gcaccggaac aacactaccc
    23461 tcagctagcc aatacgctgt tagtttgtgc gacagaggta cgcactgcag aggatatcgc
    23521 gaaatatgca aaaacattaa aaaccataat gtccaagcga ggtgcctgat gtttattgtg
    23581 caattaacct atttagttcc catcaatgaa gtaaacaaat atctgcaggc acacagggag
    23641 tttctagatt accactacaa acaagggcta ttggttgctt ctggccccat gaaacctcca
    23701 accggcggga ttattatagc agcgacaaat gacagggccc atttagagtc agtttttaaa
    23761 caagaccctt attatttagc agaaattgct gagtatcaat ttatcgagtt tactccagta
    23821 aaacacagga atgaactcaa ggaacttatt caaaaaacgg aaggcaaatt atgttgattt
    23881 ttgaattatc taaaactggt cgccaggcaa aagcccaaat acccagagca gtaagcaaaa
    23941 actattcaat acccgaagaa tttcaacgaa aatcgccccc aagattaccg gcctgctccg
    24001 aattgcaagt ggtaagacac tttacctgcc tttcccaaaa gaatttttcc atagatacta
    24061 atttttatcc tttgggctca tgtaccatga aatacaatcc ccggggtgtt cataaagcag
    24121 cttcgcttcc cggttttata aatcgccatc cgttagccat ggacaatgaa agccagggat
    24181 ttctggaaac tctttataaa ttacaaaatt atatttctga aattacggga atgcccggtg
    24241 tttcactaac tccaatggca ggttcacagg gagaatttgc cggagtcgcc atgattaaag
    24301 cctatcacca gtctcgtggc gacaccgccc gagatgaaat tctcattcca gatgcggcgc
    24361 atggaacaaa ccctgcctct gcggtaatgt gtggcttcaa agtggtagaa atagctaccg
    24421 ctccggacgg tgacattgat cttgacgaat taaaaagaaa agtaggtcct aggactgctg
    24481 gaattatgct aactaatcca tccactttgg gattatttat gcgtcagata aaagaaatag
    24541 ccagccttgt acaccaggcc ggaggattat tatactacga tggggctaat ctcaatgcta
    24601 ttttaggtaa agtaagacct ggtgatatgg gttttgatgt catgcatctt aatttacata
    24661 aaacatttgc tacacctcac gggggtggcg gtcctggcgc tggccctgtt gcggttggca
    24721 aacgccttat tccttacatg cccttacctg ttgtgaaaaa aaccgactcc ggatatcact
    24781 gggcaactcg ccaggattat ccacaaagca taggaagatt atcgtgcttt atgggtaatg
    24841 ccggcatttt attacgtgct tatttttata tgctggtcct tggtaaagaa ggcctattgc
    24901 gcgtatcaga gttcgcaaca cttaatgcca attatttact taaagaattg actaaagtgg
    24961 gctatacagc agcctatcct gacagacgtg catcgcatga atttattctt actttaaatt
    25021 ccgaaaagaa aaattatgac gtgactgcca tggattttgc aaaaaggtta ttggactatg
    25081 gggttcatgc ccccactact tatttccctt tactagtacc ggaatgcttg ttgattgaac
    25141 cgcctgaaac agaaagtaaa gaggaattgg atgcttttgt ggccgtgatg aaaaccattc
    25201 gcgaagaagc tagcaaacaa ccagatatac tcaaagctgc gccccacact ttaccagtca
    25261 aaagactgga tgatgtgaaa gcggctcgcg aactggattt aaattatttt gcaactcacg
    25321 agtaacctgt acaacagatt ttcccaattt tagcgcagca gctttttttt gatgacagtt
    25381 gctgcgtttt atgacggcca atcttctctc gccaaaaatc tgatgccctt ttataacaat
    25441 tcacaaattt ctgcattcca agactctcaa gcatcgcatt attaacttta taaaataatg
    25501 ttataatgtc attctaatcg agcaaataaa tggttgtaga attggatgag ccaccagtca
    25561 gaacgttgct agcgcgtcag ggaatctatg ataagaatag cgcaatcttt gcttatgaac
    25621 tcttatatcg taatagtgaa gcacaaaatt cgcaggtaga taatttaaat ccgttgtcag
    25681 gtgaagcagc tacctcgtct gttctattgc agttatttgc caatcttgat gtaaatgcca
    25741 taataggcaa taaacaagcc ttcattaatt tcacccacaa ccatcttgta caaaaaatcc
    25801 caatgttgct tcctaaaaat agaatcgtca ttgaagttct tgaaacggtg gcgattgacc
    25861 aagcattact gttcaattta atggaattaa agaaacaggg atatcaaatt gccctggatg
    25921 attttgtctt caggaatgaa ttagctcctc tggttgacat ggccgacatt attaaaatcg
    25981 atgtgcttca tcttgaccag caacaaatcg cagaacaatt attaccgctg aaatctttta
    26041 gaggtaagtt actggcagaa aaaattgagg acaaacaaca attcaatcat tgcgtcaatc
    26101 ttggatttga tttttttcaa ggtttttttc taaacaaacc ggattcctta acaggacaaa
    26161 tcattactga aaataaaata cagcttttaa gattattaac agaaataaac aatgaagagg
    26221 tgccaatcca acgcattgaa gaaattatcc tgcaaattcc caaactaagc tatcgtatct
    26281 tgcgtttggc aaattctgct tctttgtaca tgagcaaaaa aatagaatca ttaatggatg
    26341 ccatttctca attgggatta atgcaaatac gcaattggtt aaacctgtta ctcttggcaa
    26401 gtctcgatga tgtcgcacct gatttgttgg agcgaacatt aattcgcgcc aaaatgtgtg
    26461 agtccttagc aaaatcaatg gattatccta actctcatca agcttatacc gtaggcatac
    26521 tttctacttt ggatggtatt ttaaatgagc ctatgccttc gctattagca aaaattcggt
    26581 taagtgaagc actcaatgaa gcgctactga actataaagg ggatttaggc aaaatcctga
    26641 aatttgccat agattatgaa caagcaaatt ttaatcaatt agaacgcatt cctgtaaaaa
    26701 gcgaaacgct ctctcaatct tatcttaagg gaatagaata cgccaatcat gtgatagaca
    26761 ttattaataa ataattgtca tcaccattat tattcattct gtattcaatc tgttttacaa
    26821 aagacaatgc tatgaaaatt gtccatatca gatagtacaa aagggatttt cataccacga
    26881 ataactcact aaattttatt taaagtcaca aagatcttaa attgtcctta tcgatcaaat
    26941 aatagcttga tgtttcttct tgtttattta taattcatgg aatgttaaat ttattctctt
    27001 atttcaaatt atgatcatac gtgaactgtt taattggata cacaatgata gagcaaccct
    27061 tcatctcagt gatcaaacga tcgaacgtga tcttattgaa caagaggaat cacaagccaa
    27121 acaaactcat cgtgtgcttc tgggtaatgt ccgtctttta aaatatagtg gttcatccaa
    27181 tacagaggcg attaaagaat tagagcagca ttgtcttttt atggattatc tccagttgta
    27241 taaacaagag tttgttaagg atggaaaaaa cactgttttt ttaattgcat taagaaatct
    27301 gttatcgcag tctcatgaag agcaacttcg tctcatgcct aaaatcaatg agttattccg
    27361 agtattatta cgagacaaca aagaaagtgc tctatctttt tatgataaaa ataaggaatt
    27421 attcaggcac tgtacagaaa ttcaagaaag ggtaacgttt gagctcctcc agcaaaaaat
    27481 ggaggctgac agtaaatcag taatgactca gctagactat ttgaacaaac atttagcttc
    27541 ccaggaaaat cccttaggaa tcattgcgct atgccgaaat tggattggtg aaacagaaaa
    27601 attcactgct ttaatactct ggttgctaga acgcaaagta aacgttgaaa aaattcttct
    27661 tactaatctg ctgcaggatt ttcttaaata tcatttattc actttgcatt ccaaggataa
    27721 tgaagtttct cgcttatact ccctgctcgg ccatttccct gaagctcagg aactcgttat
    27781 ggcagcccaa aagataagct gtggagaatt aatgttccag caatactctc ttgacggcat
    27841 ttttcggggt aaaaatcttc cagttgtttc accagaaatc ccccctctgc aattttcctt
    27901 aaaaaaggac aattttaatg cattatacca aacttttggc caagattttt taactgcagg
    27961 agttgctact gcaacaaatc atagcgaagt agcttggctg gatatgttaa ggcatacact
    28021 gaaccagcca gaaacactca aattactgcc tgatattatc aatattattg ccagagagta
    28081 ctcaccgaaa atcttaaata cccttgcaga actcattgcg gagtctactg cccatcaact
    28141 gcttactctt aaccaaagct gtgtttttca tttactacag cataaaccaa gattacttca
    28201 cgatattaca gaagaaaatg ttattgggta tatcaatcat ctggttcgac tggatactca
    28261 tgatcaggaa atcatttatc agttaatggc tttgtttagg gtgttattga agaaaaacca
    28321 tccggcaacg aaagcagtat tcgaagcaat tctcgataat cttgtaaatc atccgcaact
    28381 gcttgaagat gaagagctat taacgcagtt aaaaaaatac ccggattgtg agctcctttt
    28441 agaggaacgt tgtgaaaata tcctaaaaca gcttaatttt tgcattgctg aacaaacatc
    28501 aggactactc tttggcaaac ataactacta catcatagaa gatgtttggc ttggagcctt
    28561 aagaaaattt gcagtactcc agcaaatcaa cccacaaatg aagtttagct ttgggcataa
    28621 gtatgccctt caagcaagaa ttgccgaagt tgtctttatc catcagggag acttgtttga
    28681 tttagatacc tttatggatg cgttggattt gccccccgtc acctcatcag gagaaatcag
    28741 tctttatgag cgagctctaa ttgaaattct tgcaacgata gataatgaac tcatcagaaa
    28801 acaaatcatt cagaagctgg aaacaatccc tttcaataga cttgactggc atgaaaggga
    28861 atatggaaat caaactgttt tcattaaagc cgcaaaaaag ggtaatttgg gcttaattaa
    28921 tttgcttgaa gacaagatga agccttctgt gcttaacaaa gcgctaagag ttgcagctaa
    28981 aaactaccaa tgggaaacat tggctcatct ttgtagtctt ccggaggttg agctaagcca
    29041 agaagaaatg gatgatcttg tggcccatct ggcaaaacat ggacgagtag agaatataaa
    29101 aaaactcttg aaattatatg attacaagcc atccacagaa ttaattagcg caattcttaa
    29161 aaaagggata gaaaatgata atttacaggt cgtcatgtat ttctgtaagc tgccagtgga
    29221 atctcccaaa caagcaatac ttgatcgcct atttaaattg gcaattcaat tgcagcattg
    29281 ggacattgtt aagtttctcg ccaattcaaa gcattattct ccatcccaaa ccactcttga
    29341 aaaggctttt caacaaactg cactagctat gcaacatgag gcagtagaaa ttcttggcaa
    29401 tgttgaaaaa acccccgtac gtgcaatagt tattgaacgg gcattactga aagccagtaa
    29461 actagggcat acaaatatcg ttcaatctat ttgtagttta ccttcggagg tattaacgaa
    29521 gcgagctatt gaagacgctc tagaacaagc agctgcgcaa gggcatttag acattgtctc
    29581 ctgcctctgc gagccaggca acacagcgct gaggccgcca gggatcaact caggaatgaa
    29641 aattgcggtc caggcaggca aattatcctt agttaattat ttctgctcga tgacagggag
    29701 caataaacca accccccgcc taatcgatca aacattagtc atgtcagcca aaaacggtca
    29761 aacagccatt tttatggcta ttcattcaaa ccgccagact cctccaggaa aacatgctat
    29821 tgagcaatcc tttcaacttg ctatcactgg agggaaatta caaattttag actatttatg
    29881 tcgtcatgaa aggtatggtc tcaatcaaag caagatagat caagcattga tttcagcagt
    29941 caaatccaaa caactggaaa ttgttagcta tctctgtgag tctctggaaa ttacacccag
    30001 tagaaaagca ctaagaattg ctgttactaa agcaatttcc agtgaccaaa atgacttggc
    30061 tggttacttg cgaagctgct ccagtggcaa atcaaagcct gtagatacac ccattgacga
    30121 gatagattcc ccttgcgaaa ttggtaaaca attagcaact aatgggcttt ttaagtataa
    30181 aagaaaagat gacaaggggc ctccactgct tttaaatcct agtctttaaa actataggga
    30241 gtagaggaga ccctctttat ttaatttaat ttcgtttaaa tttttgtata aacagatttt
    30301 tttatatgaa atgatatgtt gttaatctat atccacaaac aataaccata ttggcctggc
    30361 agtcactaag ccatgtgcac tcccgttaaa tttgtctgat tcaatgactg gttatagaga
    30421 gaaaatggct tcatggggaa atcaggtcag gaaattgctc agccaatttc aggtataatt
    30481 ctgtttgaaa ccgcacacaa atttgggcag tttgcaccaa acatctccaa tcaccatact
    30541 tggacatcca gctcagacaa aatcagagaa tccatctcat catcgtaaat tttcttaaac
    30601 caagttggta tttttttcgt tgactcagaa gcataacatg aaccttcttc atactgtcct
    30661 ggaagatagc tgttaaataa tttttctaaa gcgcggttat catcaattaa cagatttatt
    30721 tggttaacca gataatacgt ttctcggggt ttaatttttt gtcggtgacc atatttatca
    30781 agccatttca atgaagccaa accaataaat aaagaccaag cacaaaataa gcgatacggg
    30841 cgtccttcaa taattgggat gctctttaca taagccagtg aatggtgtgc atgaataacc
    30901 aaactctcgc gcaagctatt ctctgaagaa ataaaataac gcccacttgt ttcatcatcc
    30961 actttatctt tcaatagatt gattttttgt aaaaagaaac caaagtgaaa ggcttgattt
    31021 aacagactgg ctgtccactt aaaattagat agtaaatcag taaatatgtg cgacaataat
    31081 ttgccaacca atcctgcaac aaaaaaacag tattgattag tggtaactaa tgaaggaaat
    31141 acgattttgc cttccatatg ataatgatta aggaaataat gcattccatc cactacttgc
    31201 aaaattgttt ttatcacaga ttgcttgatg gattcaggta aattatcttt ttcttccaac
    31261 aaaagaggga cggcacatag aagttgtttc tcccctaatg gaagctgcga aggaaagctg
    31321 gaaagccatt ttgtgaattg atcgtttgcc ggtgtttgaa ctaaaaatga tttaaaagca
    31381 gcaaatgaat cactttgtgt ttgaagatct tgccatttgg catcttcaat actgtctgca
    31441 acacgcaaaa gtaaataact caacgcaacc cattcttttg ctggagaagt taactggctt
    31501 atacaaaaac taaaacttct gcttacattt tctagatgtt ggtaataaaa agaaaacgat
    31561 cgcataacca tcagccaagt tgatgttcgc tttttgataa tgcctgtttt attctctcgt
    31621 aattttcttc cactgttttt gataactcat catatgaaat ataatttttc catacatgca
    31681 ttaaagaaaa taaatacaga aatccaaact ccaggtcgtt ttccaataag gtattactgt
    31741 acttgataaa gttatctaaa tttgaaaagg gttttgacag tggctttatc ctggattgct
    31801 ccatgtcttg aaacatttga tcaaaagcct cctgcatata acgcgggaac gggatattgc
    31861 catcaaaaac tcggtgatca acactcatgc ctacaggcaa aacatcttgc acctcaaatg
    31921 tgttatttgt tttattccag acttgttttc tttcgatttc tgtcagagtt attttaaacg
    31981 tttcatttgg aaataagggt gaaaccgctg cttgatatcc ccaatgacca atattgctca
    32041 aagaaatagc aggtctggca aataagaaat cacggtacac tcgctcatgt cgtggagtaa
    32101 gtaagcgatt gactacctcg attaaatagg gatgctcttt ttgtaaggat tgtgtttttt
    32161 cataacagta cttcataata cgcatgtcat gctgaatgtg ttgagctaat tcagtcatag
    32221 tcatctcatg gcaatttttt aactcaatcg cacctaattg atcactacct ggaatcttta
    32281 ccgcaagcgc tacgtaacta ttgtgcgagt ggtacagttt atgattgaaa atataatttc
    32341 tcgtctgcgg attatcaaag caagctttac tcaatgcttg taacatgagg caagttattg
    32401 tttccggttt aaatttcttt tcaaaaaaac aaacaggagt aacatttata ttaaaaatag
    32461 cagactcagt tggtaattcg gggtcattcc aaatcgctga aagtaattta cgaatattgg
    32521 tcggttcaat ggctttgtct ttttcaaaat catgcgataa attcttcatc aataaaggca
    32581 tagtatccat aaacaattgc caatgggctg aaggagttaa gccaattttt ttaagcatcc
    32641 aatggtgttt attattgata ttattgagca gtttcagtgc cccctctttg cctaatagaa
    32701 taccaagggt agtcaaatat tgctcatgcg caaatacggt atttatcagc tcatcttcaa
    32761 aaattgctaa ttttttcctt aaatagtctt tatttggcaa cccaatctga cgatataaat
    32821 cgtactcgtc catatgccgg ctttcatctt caaggactgt ttcaaaaata gttactgcga
    32881 tgtttttctc tttcattgca atgcataatt cttctaccca tccttctcca accaaattga
    32941 ccaaagtgat agaagtggcc agatcctctt cgccttcaag agaagaaata aaatgtttga
    33001 tactcttttg atggccaagt ggtaatgcgt aaggagcgga caactgaaac gcaacttttg
    33061 caaaaacagt ggcatggaaa atctcgtcgg taatttgttg acgcatttgt tgatacaaat
    33121 ctgttccacg gagtttctct agagatttta taagccctct taggccaatt gtttctaatt
    33181 gagcaaacaa ggaaaataaa tgaatataga taagcttgtc atgctcaagt tgaaaaggaa
    33241 tttcgttgta aaatatcttc tccattcggc ttttgatggt ttccttttcc tcgtcattca
    33301 atacagacca agcattattt aaagactgct ccgcagccca agttggtttt aacaaatcgc
    33361 ctaactgaat cataaattaa ccccaatcca tttgttatat tgcaaacgcc tacactcttc
    33421 cggagcacat cctaatattt aaggggaagt gtactattga aagcgttttt tgtggatatt
    33481 gtcatatact gattgtaatt gctactggat tccagcctga aaataaccat agataaaaaa
    33541 agacttctta aaattatctt atcgatgagc gataaaaccc ctttgatcaa tgagttattc
    33601 gcaaatctcg agagtctggg ataactggat ttcggctcac tccgacttct aaacaggatc
    33661 gacaaagtac gacctgatat ctcatacaaa ccaaagtacg ttagctgatt gacgagtcat
    33721 ttaaaaatca aatctctata ataaaataag catcccagaa ctgtttgcaa tgcagcaata
    33781 taacaaaagg gacagattag agagaggagc tattatctca atggaaaata attgatttta
    33841 atagcaaaaa tatctttgct attaaaaaaa aataagttat aacactctta actacatcaa
    33901 ggattgatta gtgcaaatgt tgggtcaaca tctcagtaaa ttcatgttga gttgactgta
    33961 taattttcat ggagtgaaaa atgaatagta aagtattttc gcaacgtttt aatcgtgaat
    34021 tggcaactct gggattcccc gaggatttaa cagaaaaaac gaaagccgta tcaaaagtat
    34081 ttggcgtcac acgccattta gctaatgcaa tgatatttgg ccacgttctt cctgataaag
    34141 aacaactgga taaaatcgca gaaattctgg aggtttgtcc tcaatggcta agtggggcaa
    34201 cggataggaa aaaagcctac tcaggccgtg agacggctga tagtgtgtaa taaacctgga
    34261 atttcatgta tactgtaagc taatagacag cgtttgctaa aattatttac tttgcaaacg
    34321 cttttttcat tgttaggtta ttaaaatcat ggaatgctta agtttctgtg tggccaaaac
    34381 catagacttg actcgtcttg atcttcatct aaaaaacgtc tctaaggaat ttagtgctgt
    34441 aaaaacccga gatgtaataa gattaaattc tcatagaaac aaagatcata ccgtgttcat
    34501 ttttaaaaat ggaactgtcg tatcttgggg tgtaaaaaga taccaaatcc acgaatacct
    34561 ggatattatt aaactgcttg tagacaaacc tgtagcactt ttggtgcacg atgaatttca
    34621 ttatcaaatt ggtgataaaa cagccataga accgcatggt ttttatgatg tagattgcct
    34681 gaccattgaa gaagacagtg atgaactaaa actcagtttg tcttacggct tttctcaatc
    34741 tgtaaaatta cagtatttcg aaaccattat tgacgcattg attgaaaaat ataatccgct
    34801 gattcaggcc ttatctcata aaggagaaat gcctataagc agaaaacaaa ttcaacaagt
    34861 tattggtgaa attttaggtg ccaaaagtga gctgaatttg atcagtaatt tcttatacca
    34921 ccccaaatat ttttggcaac accccacttt agaagagcat ttttccatgc ttgaacgtta
    34981 tttacatata caaagacgtg tcaatgccat taaccatcgt cttgatacgc ttaatgaaat
    35041 tttcgacatg ttcaatggtt atttggaaaa tcgccatagc catcacttgg aaataattat
    35101 tattgtatta attacagtag aaattataat tgcggttatg aatttccatt tttagggttt
    35161 ttcccttaat ttatgcccac tctaaaatcc tattttctca aagacacctc tttgcgcaca
    35221 gaaatacttg ccggagcaac gaccttcctt accatggttt atatcgtttt tgtcaatcca
    35281 aaagtcctgc acgatgcagg aatggatcaa ggcgcggtat ttaccgccac ttgtttgata
    35341 accgctttcg cctgcgcatt aacaggcttc atagccaaca caccgatagg tgtagcgcca
    35401 agtgtggctt taaatattta tttctcttac agcgtagtac aagggatggg agttgactgg
    35461 caacatgcct tggctatggt attcatttcc ggcttgcttt tcctgtttct taccctcacc
    35521 aaattaagac gcctcctgat tgaagcaatc cctcataatt tacaaatagc cattgttata
    35581 ggtatcagtt tgctaatcgc cttaattgct ttagaaaata atcaaatcat tattggtgac
    35641 cagcacaatt taatgcgctt agggcctatc atcagaccag aaacaggact ctttttcctg
    35701 gggtttattc ttattcttac tctggattat cataagatac caggtgccat catcattggg
    35761 atactgagca tcagtgtctt aagcttgatg ttgggcttga ccagttggca aggtttgatt
    35821 gctatgccac cgtcgctcga acctacgttt ttgcagcttg atttttctgg tctttccagt
    35881 atcgtggctt taaaaaccat ttttaccttt tttctgatca ccgtgtttga tgcgacaggc
    35941 tcattaattg gcttactaag tccaaccatt tttaaacatg aaaaaaatta ttataaacgt
    36001 ctaactcgca gtttaactgc cgatgcagcc gcatcttccc tcgcaggcct catgggctct
    36061 gccagtacct cgccttatat tgagtcagca accggaattg aagccggtgg gagaaccggg
    36121 ctaactgcgt tagtcattgc aactggattt attctgatgc tgtttctttt tccacttgcc
    36181 aaaatgattc caagctttgc ggttggaccg gctttactct acgtagcgtg ttgcatgata
    36241 aaacatatta ctaatatgaa attacaggat atcaccgaaa cagcgccctg catggtcatc
    36301 atcatgatgg tgccattcac cgcttcgatt gccgatggaa taggtttggg aattatactt
    36361 tatacccttc ttaaattatt tactcgtcaa aaacccaatc ctttgattat tgttttaagc
    36421 cttgttttta tcgttttctt tttgattaac taagtgaatg tgatctttaa atcctggata
    36481 agccgaaaag cttccataaa aaacttgtct actctcaaaa actgcaaaca aaagtttctt
    36541 ta
//
LOCUS       DASLOE010000015        35983 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.015, whole genome shotgun sequence.
ACCESSION   DASLOE010000015 DASLOE010000000
VERSION     DASLOE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 35983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35983
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.015"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..3882
                     /locus_tag="U6309_000585"
     CDS             <1..3882
                     /locus_tag="U6309_000585"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012321.1,HMM:NF012573.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HEN5504040.1"
                     /translation="VTVTDGDGDVATQAIGIGDKVVFEDDGPVASNITRSGLANEGAD
                     TNLMLILDTSGSMAGSGIQTLINSTLELLERYEALGNVKVRIVTFNTSATAIGSVWMT
                     VDAAKNALLGLTAGGNTNFDAALITAMNAFNSGTVGGADGRIGGAQNVSYFISDGNPT
                     VNQDWPSIPGTLAQNGIQPSEQAAWEAFLQDTNGASAGGEKIRSYALGMGTGITVSNL
                     EPIGFNGVNGTDIPAVVVTDISQLDSVLASTITASPISGTLISDPNPGVNFGADGGHV
                     QSVTVNGITYTVNANLSSLSTSGSATQGSNTHSYDNSTHVLTVTISTGESLTIDMDNG
                     SYTFTPPANITSSINRIINYVLVDGDGDTAAATITFNIDISGFNDPLVVRDDLVLTNQ
                     PAVSGQDVISIPTWALLANDTGGSGVSSVSSVTQPVAEGTVVLNAGSVAFTEESNSAA
                     DGTYNGSTSDPFRYTNTTGSQSATAKVDLDRTQAGENRLDGTYRSEILLGRDTSNDTI
                     IGNDGNDILIGLGGNDTLSGGNGDDILAGGAGNDTLDGGAGNDTATYIDATAGVTVSL
                     NTSSAQNTVGAGTDTLTNIENLTGSKFNDTLTGNNSANTIKGLEGNDTISGLGGNDTL
                     DGGSGDDVIIGGLGDDILTGGSGADQFVLVKGQGGASAGAAPIDTITDFEVNIDKIVI
                     NGNNITGVSVSTPVSNTYTITVNYSDAATEHFKVTLSNGAVLNDSGKTHLNGVVISGG
                     TATIDGTIVGAVLYLDLNANNQEDEGERLGITNQYGHVEWVVDLSKFDVNGDGQYVIG
                     EARAVQTGGFDIDTGLSYEINLYGPVGSAVISPLTSLLQAQLEAGMDYETANAALVAR
                     LGLPEGTQIISFNPITGSGEVLAQNAGVMTAAIQFAEIAAIHYSTDEGHVSFAVFEAI
                     SKALLELPEGVVADFGDEGFLQSISNYLGLDALVTSDMIDYMATSQKALEASILTLAP
                     GEDALTAISKIQHVTQGIYAQAIEMALIGYLPLDALKNMSAVLKAYADGDITDEQLNS
                     FEDKLSAVIVNWEDNNVTDTSHQATLQTERTVDDSSVKSEVNHEMAQEVNAVINDFMD
                     EHNISLYYYDQQAQMPQEAQQPTEDQAEETVMHDDVVQDLLLDDHSLTMLANNPELNG
                     GNGNDILHGTTGNDFIRGGQGNDTMTGGGGVDTFFWLSGDDDGGVDTITDFKANPVDK
                     SSDASVLNLSDLLSDADLETNSLDNYLNVSTTEEGDTAIKVDPNGNGNFDAPAQTIIL
                     EDVDLTAVFATNNSHDIVNQMIANGNLIVEQ"
     gene            4061..4660
                     /gene="wrbA"
                     /locus_tag="U6309_000586"
     CDS             4061..4660
                     /gene="wrbA"
                     /locus_tag="U6309_000586"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEN5504041.1"
                     /translation="MSNPYILVLYYSRTGSTAQLAQYIARGVESVSGLEARLRTVPPV
                     STTCEAVDKPVPDAGATYVSLDDLRHCHGLALGSPTRFGNMAAPMKYFWDGTTSLWLG
                     GDLVNKPACVFSSSASMHGGQETTLISMMIPLLHHGMILLGVPYTEPALTSTQSGGTP
                     YGVTHVAGGDNENPLSQDEISLAKALGERLGRVAMGLKV"
     gene            5113..5463
                     /locus_tag="U6309_000587"
     CDS             5113..5463
                     /locus_tag="U6309_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEN5504042.1"
                     /translation="MVPADAKLLKQAKSLRKRSTDVENCLWYYLRAHRLKGYKFKRQV
                     PIGNYIVDFVCMQRKLIVELDGGQHLLNKEYDKDRSAYFSTLSYKVLRFWNDDVLLRT
                     GDVLESIVRCLEES"
     gene            5519..6301
                     /gene="xth"
                     /locus_tag="U6309_000588"
     CDS             5519..6301
                     /gene="xth"
                     /locus_tag="U6309_000588"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEN5504043.1"
                     /translation="MKVISFNANGIRSSARNGFYDWLVGQDVDFVCIQETKSQVEQLI
                     PEELYFPRDYFCDYFDAEKKGYSGVAIYSKYKPVRIVKGLGFDYCDKEGRYIQFDYPK
                     LSVVSLYLPSGTSGEDRQEVKYAFLEQFARHLMNLKNEGRELIICGDYNIAHKKIDLK
                     NWRGNQKNSGFLPEERAWMDELFGSMGFVDAFRVHNQEEEQYTWWSYRARAWEKNVGW
                     RIDYQVITPGLTEYVADSRIFREARFSDHAPLMIEYKGDWCV"
     gene            6294..7064
                     /gene="xth"
                     /locus_tag="U6309_000589"
     CDS             6294..7064
                     /gene="xth"
                     /locus_tag="U6309_000589"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEN5504044.1"
                     /translation="MFKLASWNVNSLKVRLEQVLQWLESSQVDVLAMQETKLTDENFP
                     VASFTDKGYHVIFSGQKTYNGVAIISRYLMTDIQTDIPELEDPQRRILAATVKGIRLI
                     NLYVPNGGELTSDKYQYKLDWLNKVTRFIQHEISIYSKVAVVGDFNIAPEDRDVHDPV
                     EWEGCVLVSPPERQAFMKILELGLHDSFRNFAQEEKVFSWWDYRAAAFRRNRGLRIDH
                     VLLSHELNALCRQSKIDKEPRKAERPSDHAPVWVELEL"
     gene            7201..7428
                     /gene="rpmE"
                     /locus_tag="U6309_000590"
     CDS             7201..7428
                     /gene="rpmE"
                     /locus_tag="U6309_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEN5504045.1"
                     /translation="MKASVHPDYQTVKVTCSCGEVFETRSTLCKDLNIEVCSMCHPFY
                     TGKQKLVDTGGRVQKFRDRYNMRTGQAKSKE"
     gene            7555..8790
                     /locus_tag="U6309_000591"
     CDS             7555..8790
                     /locus_tag="U6309_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEN5504046.1"
                     /translation="MDNEVIKQRALDYHEFPVPGKLSVHLTKSTNSQDDLSLAYTPGV
                     AAPVLAIAEAPENAYRFTSKGNLVAVMTNGTAVLGLGNLGPLASKPVMEGKAVLFKRF
                     ADLDVFDIEIDAEDPQSFIATAKRIAPTFGGINLEDIKAPECFEIEQALIQQLNIPVF
                     HDDQHGTAIVVAAGLLNALELQNKKLSDIKIVCMGAGAAGIASMRLLVALGAPKSNML
                     LLDTKGVIHDGREDLNPYKFAFARNTPCRTLEDALVDADVFIGVAKPDLLNAHLLSLM
                     APNPVIFALSNPDPEIKPELAHSVRKDLVMATGRSDYPNQVNNVLCFPYIFRGALDVR
                     ATCINQAMQIAAVEAIRQLVHEPVPQVVKDNYPGVVNWDFGPDYIIPKPIDPRLKERV
                     PAAVAKAAIASGASQFNNL"
     gene            9001..10293
                     /locus_tag="U6309_000592"
     CDS             9001..10293
                     /locus_tag="U6309_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5504047.1"
                     /translation="MHTENRTNYALIGWLLCGLGAIFYSYEYLLRIAPSVMEDALRSH
                     FNLSASGFGFLSSFYYFAYVPMQLPVGVLLDRYGPKRLLTLACLVCVIGTYMFTGTTI
                     FWVAATGRFLVGFGSAFAFVGVLKIATLWLPENRLAMISGMTSALGPIGAMLGDNFLE
                     LFIVKMGWVKTLNMTAAFGIVLTLVLWVGIRDRKGQHRQSGTVPTLKKGLVDLGIIIT
                     SRQIWVNGMYGCLVYLPTTVFAELWGIPYLHHAHGLTTQAAGLANSVLFLGFIIGAPI
                     MGYISDRLYRRKFPMLIGASGAAIVMMMILYLPGLNESNIQALMFLLGLLYSAQAIVF
                     AVGRELSPGEAAGTAMAVTNMIVMLGAMFLQPLVGHLLDFSLSTHLSDTTGIVVDNMS
                     KLYTVDDYQFALSIIPLGILIAAILTFFLKETHAKAPK"
     gene            10274..11551
                     /locus_tag="U6309_000593"
     CDS             10274..11551
                     /locus_tag="U6309_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5504048.1"
                     /translation="MQKHLNNMTTKKQISYGVLVCFIGAVFYCYEFILRIVPGALQTE
                     LSTALGHISATTFGQISALYYFAYSPMQMPVGMLMDRYGPRRLLTFACLCCALGSWMF
                     TLTWSIVLVGIGRFLVGFGSSFAFVGVLSLALHWLPRRYFSLVAGLITTLGMLGLVYG
                     EIKITEWSEVIGWDHVLMHIAMIGSALCILTFFVVRDGPEGYQPNKYPWPEFFHNVLK
                     VLTSHEVWLIGFVGACLYTSLSVFGELWGKTYLEQAHHLTKVEAAKTVSAVFLGWAVG
                     APLAGYISDITRRRLLPLVIGAIVSLICISFVLYCPGLSYLTLNILLFLYGVFSATEI
                     IVFIMAKECSGAKLSGTVFAVTNMIISLAGAIFQPLVGKLLDTFGDSGIVAGEHIYTV
                     DDYQVALSILPISLLLVTILAFFIKDKDHSNNS"
     gene            complement(11582..12400)
                     /locus_tag="U6309_000594"
     CDS             complement(11582..12400)
                     /locus_tag="U6309_000594"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEN5504049.1"
                     /translation="MVKIRPFLKWAGSKYNCLNEIISSLPTGQRLIEPFAGSGVVFMN
                     TSYSSYLLAESNPDLVNIYRILQLNGESFIDYCQQYFTPEANCKEKYYELRTEFNNLM
                     DSNQKSAVFLYLNRHGYNGLCRYNSKGKYNVPFGLYEKPYFPRKEMMLFYKKCLSAQF
                     IHDDFRNTFALAEPGDVIYCDPPYVPLTENNQTLPYNQKLFSLEDQIELAELAQETAS
                     KGIPVIISNHDTEFTRHHYKKAQIKSFYVPRWISCRSQMRLPVKELVAVFNCPS"
     gene            complement(12571..13767)
                     /locus_tag="U6309_000595"
     CDS             complement(12571..13767)
                     /locus_tag="U6309_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan/tyrosine permease"
                     /protein_id="HEN5504050.1"
                     /translation="MINSKLIGGILLIVGTSIGGGMLALPVSTAEVGFTNSLFFLFFC
                     WLVMTAGALLILEVNLRLPAGSNMISMAKSTLGLPGQVIAWITYLLLLYTLLAAYISG
                     GSDVFSSLLHKAHIDLPNWITSVIFTAMFSLIVYSGIRSVDYVNRGLMFGKLGVYILL
                     VIIISPHVSTAGLTGGSAKAFTGTLMILVTSFGFASIVPSLRDYFNDDIRSLRRVILF
                     GSLIPLFCYIVWDAVIMGVISREGENGLLSLMNSDHATSGLTEALSQSVSSQWISGFF
                     GFFTSICMVTAFLGVSLGLFDFLADGLKLKKSGNQGKYTLALTFIPPLAVVLFNPGIY
                     LHALSYAGVCCVILLLLLPAIMAWKGRQTCSQSPSETHLIPGGNPALSLIGFIAIGLL
                     IIAIMY"
     gene            complement(13970..15160)
                     /locus_tag="U6309_000596"
     CDS             complement(13970..15160)
                     /locus_tag="U6309_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan/tyrosine permease"
                     /protein_id="HEN5504051.1"
                     /translation="MRSRFIGGILLIVGTSIGGGMLALPVANAATGFWQSSLFLLICW
                     VFMTFGAFFILEANLYLSRGKHMVSMAKATLGDYGLLTAWISYLFLLYTLLSAYISGG
                     ADVLNSLLSKVNWHLSDWQASSLFTLIFGLVVYGGIHSVDVANRALMFGKLAVYCILV
                     ILIAPHIEFHYFQHGDYRQIGGAIMILITSFGFAIIVPNLRDYFDDDIKTLKKVIFIG
                     SLIPLICYIAWDAVIIGSLPSEGTSGLESLMQNPRTTSALASLLTDKVENTTISALFN
                     FFTSICMLTAFLGVSLCLFSFLADGLKLRERGRHGIGLFVLTFLPPLLIVIYYPGAYI
                     YALRYAGIFCIILLLLLPALMCYFGRKRYKSPFWVPGGKISQFAVIIISVVLLAHEFI
                     ALFE"
     gene            complement(15171..15863)
                     /gene="cmpA"
                     /locus_tag="U6309_000597"
     CDS             complement(15171..15863)
                     /gene="cmpA"
                     /locus_tag="U6309_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-OmpA-like family protein CmpA"
                     /protein_id="HEN5504052.1"
                     /translation="MSRKLAKTRILGYGLMICFLAGCFHPPYNNFQPDRRAVKRVGVD
                     TGIGAVAGVIASGTASGTLIGAAAGGTVGLVASIYRDSKRKIIRDLQKQDIQYVEYGD
                     TRTLIIPTDKYFMFSSPRLNEICYPGLNNVIRLLNFYPQSTIYVAGFTDNVGSRSHKR
                     KLSQAQAETMMTFLWANGIAAKRLKAEGYGDKNAISDNAIIHGSAQNRRIEIQWFTSP
                     AQPPQPQMAYVK"
     gene            16094..21301
                     /locus_tag="U6309_000598"
     CDS             16094..21301
                     /locus_tag="U6309_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN5504053.1"
                     /translation="MKIPVQLKNIGGNIPENDIALQVSYFIKSQNDELLPQSDAYIAP
                     ASGKIDVSFGNNLARNKDVFFAVALKSNNILAQTSTSATQLKDEVPITMEFDYSPLVL
                     SEPDDQIVSPRPLFVYGRLLDKQGKKKLEDVQIIFEATRANDGGLQPIASVRTEADGY
                     FFIEYPQGSFIDASARVGLPLRENPIPVRLDEVRIDGDISNKVFPRNVILVAELMEAE
                     EATVEKECGCEVLDVHESKRILEEYSFYSVVRTTEPEILGYVLKEEDEITLDEVLTQH
                     PIRIWEIIDPIFQLPAFSHLANRARVNLPEVTERSINTEITTRAHGRANTAPNPPQDN
                     ELAETLKQIKINRGVLKNFLQRETSITKDNIVNLIEMNESYRFKQKMTLGETKPLGRV
                     VLNSENSVDWDLETTLYQAVSVAHGHLLHFKSEWIADGYSLGDLLYSLPLAPGQKKQI
                     VVFDWERRESATNIQSLEYEESLYNSLSRDRDIFEITKGVIEENIKGKSSATTASASA
                     GIGGVVGGLLFGVSGGVGHSGSTASQNSLRQTSASDLQKIRDRIVQSANAVRSQRSSV
                     IQTVSQGERFEVSSETVANYNHCHAITIQYYEVLRHFKVRQRFAEARECLFVPLLMSQ
                     FELNKLLRWRESLQFALLNTNLSKGFDAAERIKNKWIGSNFPGGTFASEKILNASGNF
                     QIKFIIRRPDDKMVEVDDYSKPIYGGQNMIIGYQKKTVEDINEANWQSLIPFLGSDTP
                     KGFYDHHLRDAKNKDEVFHRLLGEKIATAFVGALTFHVADETGNEISSISFDTTLTSK
                     YRKEGVLNVSVRFMGPSQFSRDKVHYIKIRCGTTNVLPDYSSIIVTSGFIRYKTPHYE
                     GFLCRFQSIYDDLSPSDGVTLYAGPSLDELRDPRKEDIALVNSLIDHLNDNLEWYHKA
                     IWQLMPAERRFLLLDGIVLPGSKGLGRSLASLVENELIGIVGNSLVFPVAKGLNLDPN
                     FGASDSLTDYYMVAAGDPVNVSIPTKGVYAEAMMGRCNSCEEKDESRFWRWEESPIPD
                     TPTTINPISTESRRAEPGNLQPTAFPNPMVNIQNAPNAPDPTGLAGTLSLLGKGDSFR
                     DITGLTQNQTNALEALKASFDATKTFGQEAAKLEIQKMMDKRLDNAIKAINNNPNLDP
                     KQKMELTEKALNAYLGAGANTTQPPKDANADKLQEGINKNLDKVNSSKSGEISVTKPD
                     GTKVNTKFDGGTTSPAIETKIGKGGITPMKQENSNACWATVATMMLRWKDSKNYTIEE
                     VLTKAGSQYLNYYKQKIGLPYTEKQNFITSLGMKGEPLANYTAQNYRDWLVEYGPLWV
                     TTDTDQSKGFSAHALIITGISSDLKSLEVIDPLQGRKFVQSFEEFANAFKELITDSGL
                     LPTTQVVHFLEKIEATEGAPTPADIVKSLNEYNPSSSETLVINMVEFNTGISGITNYK
                     DWKTDGSVIHNKRKNAYRDPFGIKHLVLHETAAESGDGFDDSNNETSHMSVKRDATIL
                     QFNDLVEFENHGSGMNTTSIGIEFVNRGWLSSSTADGGEGIPAKESSLTAAQKETYKE
                     ANGYLWAFWGYGFNIYRVPPSIDQLEKEVELVKWLTRDFPALLQSISGISIYNLFPSI
                     DDTWLQLVSYAEVKDIWTFKAADIPPEAERTEKNLFVMTTGYEYLEPSYLTDKSGIIS
                     HNAFYENHSDGSFLTLYTWLRLEKGKSKTDALDIAKKLMKDHFIRVSLTSNTDKKIIL
                     LNVKDGNLV"
     gene            21695..22540
                     /locus_tag="U6309_000599"
     CDS             21695..22540
                     /locus_tag="U6309_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEN5504054.1"
                     /translation="MSKKAIAISIVIALFFITAIWFAYSFVKQEENQNWLTLYGNIDI
                     RDVSLSFRVSGRILSMDVDEGAHVKKGQVLARLDKDTFIANLEMAKAQLEQAIANQKN
                     ASRTYKRREQLVDDGAVSKALFDDAVAMKDSSSAEVSVAKANLEKAQISLNDTEIHAP
                     THGTILTRIQEPGAIVNVAEPVYTLAIDNPVWVRTYIDEPFLGRIYPGQDALVYTDSN
                     PEKPYKGHIGFISPQAEFTPKIVETTQLRTDLVYRIRVVIDQPDNGLRQGMPVTVKIH
                     LKANS"
     gene            22543..24285
                     /locus_tag="U6309_000600"
     CDS             22543..24285
                     /locus_tag="U6309_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN5504055.1"
                     /translation="MNSSKPLVSINGVSKCFPGNTDPALDNVSATIFEGQITGLVGPD
                     GAGKSTLMRLICSLMLPTQGTITVDSLNTRTDSEKIHAITGYMPQKFGLYEDLTIIEN
                     LRLYAELRNLHGEKREEQFRTLLKFTALEPFQKRLAGNLSGGMKQKLGLACALMGEPK
                     LLLLDEPSVGVDPISRRELWTMVQGLLGRGMGVVWSTAYLDEAEKCDQILLLNAGKPI
                     YHGKPGDFLQKTKDRTFQICGVSLLNRRQALMNALNTPEVIDGVIQGKNIRIVISDKK
                     IKPSLDGITREPGVHFQEVESRFEDAFIDSLKTKISGRSLLAEHIAEKPHSDKPIIEA
                     RKLTKRFGSFTAVSNNEFTIKRGEIFGLLGPNGAGKSTTFKMMCGLLQPTQGQAFVMG
                     LDLKTSSSEARSRIGYMAQKFSLYSHLDALQNMRFFSGLYGLSGERQEAQINSMIEIF
                     DLKKHLKQNSGDLPLGFKQRLALACAVMHEPDVLFLDEPTSGVDPLTRREFWTHINGM
                     VNKGVTVMVTTHFMDEAEYCDRIALIYRGKNIATGTPDDLKDKVRNSTLPNPTLEDAF
                     IELIRLDELREPVH"
     gene            24303..25424
                     /locus_tag="U6309_000601"
     CDS             24303..25424
                     /locus_tag="U6309_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN5504056.1"
                     /translation="MNIRYLKALVIKETLQIIRDPSTILIAFVLPLILLFIFGYGVNL
                     DTDQVKFGLALESYDSSTVSLSQSFSSSRFLDVSISTNRQAFRYGLVNGTIRGFIDIP
                     QRFTADSLSGNPKPALQNIVDGSYPQVAGFAENYALGALQTWISSQAYEHGQMTLKSG
                     INLEPRYWYNQELKSRNFLIPGSIVIVMTLIGTLLTSLVIAREWERGTMEAMMATPIS
                     IVDIILGKLIPYFVLGMGSMFLCTIIATWYYGVPFQGTLLALTLVSAVFLVAALGQGL
                     LISTLARDQFVASQLALMSAFLPSFILSGFIFEINSMPKWIQLLTHLFAARYFVTSLQ
                     TLFLTGNIWSLLIRCLFAIALIGFAFFFITAHKTRKRLD"
     gene            25424..26548
                     /locus_tag="U6309_000602"
     CDS             25424..26548
                     /locus_tag="U6309_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN5504057.1"
                     /translation="MAKLSPATRIFALVIKEILTLFKDPKGRIVLIAPPLLQLLIFSF
                     AATMEIKNISIGILNQDSGKHGYEVIQRFIGSPTFSKVFFIQKQSDIKPIIDNQEAMV
                     VMTIPQDFSRKIESNSQANIQLILDGRRSNATQIVNGYISSIIENYNQEIQKTHGAIM
                     TPPVMVTRSWFNENLLYLWFTVPSLVCILSMLISLVVTALSVARERELGTFDQLLVSP
                     LTPYEILIGKTIPAIIIGLAEGLLMWVAAVWLFDVPFYGSPLLMVYILFIFIMSTVGV
                     GLFISSMCKTQQQAILGVFIYMVPVVTLSGYASPVENMPEWLQDLTWFNPLKFTLIAV
                     IGIFLKGMSALEVFITTWPLLLVGAVTLFIAGWFFKQRME"
     gene            26557..27996
                     /locus_tag="U6309_000603"
     CDS             26557..27996
                     /locus_tag="U6309_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEN5504058.1"
                     /translation="MDRKLALLFSISCLFGCTVGPDYQRPDIKIQEKWPTSKVEKKIS
                     SSAPVHLTWWKAFHDPLLDRYINEAATNNLDIKAAEARIRQARAMKQISLANFYPQIN
                     GSASANSLQISKGTLEGIFANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEAS
                     KASLEEAYESRRTILLSTIAEIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYK
                     AGVSSEVDVSLAEGQLKNTESVLPNYITQIQVNAYQIAVMLGKDPQTILKDIAKIQSL
                     PKPPQKVPMGLRSDILRQRPDIRATERQLASAVAEIGVAVADFFPQFNLSVSNLAFQS
                     TSVDTLFQSSSGFWLMGPQVSLPIFHGGQIRANVESKKAVAQQAAIQYKKIILQALQE
                     AQSALVRYSQSLDTNHSLRAAIIANKRAVQFAKKRFQYGEDDYLSLLQSEAALLNAET
                     QEVNSEVSSLTALISLYKALGGGWENFEA"
     gene            complement(28328..30109)
                     /locus_tag="U6309_000604"
     CDS             complement(28328..30109)
                     /locus_tag="U6309_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEN5504059.1"
                     /translation="MKKLLIFLCGLLCSFNLFALSGQAYMDRFNRYLAWYEQLPINPS
                     PEFLEFVKENTPLANKLRDKWLYELARIKDWEDYNKYYQPTNDINLRCFAQIAKFNLG
                     LYKEAIAGSIPIWLSGESRPQACNTLFALLLKQDNFDQKFITQRIALALDKRNVLLAR
                     YLLKQYKTPHDTEIKNLTSVYENPSNISKLNPGKLNDYFYLYGLKRLVSINMDKAVKL
                     WQEKKTNIMLNERQKQDFLAHLALYKAMRNHADAQEWFAKIKPEYYTDVLVDWQIRFA
                     LKNKNWKQVEKLINNSPNKEMPCWQYWLARSLEEQGKKAEAIKIYEPLAKNRHYYGFL
                     ASKRLKKRPSFDNEKPTTNTEILKPYQPVIEQVKNFYNSKQLLQASRLLNDFISELPK
                     EEASALVYWIATELKWHGKSVYLSNNDTLNNQLALRFPLAYKDTIKVYASKYAIPPEL
                     VYAIIRQESGFREDVTSSAGAKGLMQVMPQTASAVSKTSKIPYSDHKQLFLSQKNINI
                     GIAYLQHLTKRFSNHPVLVAAAYNAGPRQVVYWLKNHPPKEIDIWIETLPWQETRNYL
                     KNVMAFYIVYQYRLNQQPDMNRFLTPL"
     gene            complement(30151..30804)
                     /gene="rpe"
                     /locus_tag="U6309_000605"
     CDS             complement(30151..30804)
                     /gene="rpe"
                     /locus_tag="U6309_000605"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEN5504060.1"
                     /translation="MTFQILPSLLSADMTRLGEEVDAVMKAGADFIHFDVMDNHYVPN
                     LTFGPAFCDALIKKFPNLPIDVHLMTTPVDTLIEAFAKAGAKRISIHPDATIHLDRSL
                     QLINNLGCEAGLVLNPATSIDTLTWCAHNLKFVLVMTVNPGFGGQKLIPEVIPKLSKI
                     RQQHPNLDLCVDGGVTPQNIASLAHAGANQFVAGSAIFNSSNYSETIKHMREQLASI"
     gene            complement(31020..31484)
                     /locus_tag="U6309_000606"
     CDS             complement(31020..31484)
                     /locus_tag="U6309_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEN5504061.1"
                     /translation="MKALIAKTYLLADKIFDFLAPIFILLTRLYLAQVFFLSGLTKIS
                     NWQATLSLFQNEYMVPVMSPTLAAYLGTACELGFPVLITLGLGSRLVLFGLFLFNLFA
                     VFSYQFLWTPDGAVGLTDHMQWGIMILMLLCTGPGKLSIDYLIRRFYLKLDN"
     gene            complement(31481..32260)
                     /locus_tag="U6309_000607"
     CDS             complement(31481..32260)
                     /locus_tag="U6309_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HEN5504062.1"
                     /translation="MRNTLLETIQKEWIAQFKEEALSSPLKNRLARSNFDGISVYMES
                     HHATLFNHLKSTFPLCMRLVGENFWYALLHRYINNCESTQYDINQYGDSLPQFIANFE
                     PAKELPYLQQLCAIEWHWHTLKLLSPVNPFDFEAFSSIDEISFQSLCFHLTPNLKILC
                     SEYPIYQIWAMNYFQQDKQIQISGCPEKVVIYQHNDLPCIMLISEEEYIFLELLSSGN
                     QTPWQKVVTELSKWISEEKIGDTLTKALRYQWINHFSLEAI"
     gene            complement(32253..33122)
                     /locus_tag="U6309_000608"
     CDS             complement(32253..33122)
                     /locus_tag="U6309_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEN5504063.1"
                     /translation="MYSNHSTDQAAPYSGCSGIGLRLEHIDDILKEQPTVDYFEVLAD
                     NYMKQSGAQFKQLLKIAEFYPMSLHSVGLSIATSSEPDYQYLQQIKNLAHCLNSKLIS
                     DHLCWTHANQFFTHELIPFPYTEETLSFIIEKTNCIQEYLNQPIMYENVSRYVTYRQN
                     TLSEAEFLNELSTATGCGILLDINNLYVNWYNHGDDPDKYLHSINTKNVWQMHLGGFS
                     KQEGYLLDSHSDKVYQDVWQLYEKAQNLFINTPTVIEWDNDLPDFSLLYDEMCKAKAI
                     IKRVAERSMNHYA"
     gene            complement(33128..33382)
                     /locus_tag="U6309_000609"
     CDS             complement(33128..33382)
                     /locus_tag="U6309_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEN5504064.1"
                     /translation="MNNISKITMAAVMAAGLSMATIPANADTPNDMEKCQGVAKAGKN
                     DCGTAQHACAGQSKTDGSTTDWIYLPKGTCEKLVNGTVKS"
     gene            33720..34745
                     /locus_tag="U6309_000610"
     CDS             33720..34745
                     /locus_tag="U6309_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN5504065.1"
                     /translation="MAKDTKIINLALQGGGAHGALAWGIIDRLLEDGRIHFDAISATS
                     AGAMNAAVLAYGFATGGKEGAREALHQFWKMVSDAGQIYNPLKKTPLEELLGIGIENS
                     MSFFMFDLMTKILSPYQFNPLNFNPLKEILEQIVDFEKIKSCKSIKIFISATNVRTGK
                     INVFDNKKISADAVMASACLPFMFQAVNIGEDYFWDGGYMGNPAIFPLIYNSECRDIL
                     ILHINPIYREHVPDTAAEILNRVNEISFNSSLMREMRAIAFVTKLIDSGWIKDEYKNN
                     LRKLYLHAIRADVSMESSSVASKLNPEWSFISHLYEEGRKKGEEWLKSNFKHLGKMTS
                     IDLDEYL"
     gene            34861..>35983
                     /locus_tag="U6309_000611"
     CDS             34861..>35983
                     /locus_tag="U6309_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN5504066.1"
                     /translation="MQQEDRAPITQKNRSFSRNTARCFDTFMDTVDFLGRIGWPVIDL
                     AFRIWIAQQLLVSAILLTNHWDTAVYLAKNEYPVPWLSPEWEALLGILAQFVGGISLL
                     LGLFTRCGAAVIIGFAVMTQVYYQPIDLNLLWIALMLGYVLRGPGPLSLDNLFEHGFS
                     RSPLPFAMLIVTFVDRTREFFSSLYLFSLRMWLMISLLLINQHIQLISSDALPLLMPW
                     LPLNSASIVFNNVSLFMAIFLGLGLATRVVAIFGLIVVLLSFTPGVTGFYLYWMVVMA
                     ILFISGPGILSLDHLIFHALKKHYPQLSGKPAFSLDGLPRVVIIGAGFGGIACAKALR
                     HMPVKITLIDRHNYHLFQPFLYQIATGSLSPADIAISIRS"
BASE COUNT        10660 a         6444 c         7783 g        11096 t
ORIGIN      
        1 gtgacagtga ccgacggcga tggggatgtg gccacacaag cgataggcat aggcgacaag
       61 gtggtgtttg aagacgacgg gcctgtggca tcaaatatta caagatcagg attagcaaat
      121 gagggtgctg atacaaacct tatgctaatt cttgatactt caggaagtat ggcgggttca
      181 ggaattcaaa ccttaattaa ttcgactttg gaattacttg agcgatatga ggcgcttggt
      241 aacgtaaaag tacgtattgt aactttcaac acaagtgcaa cggctattgg ctctgtttgg
      301 atgaccgtag atgcagctaa aaatgcactc cttggactta ctgcaggagg caatacaaat
      361 tttgatgcgg cacttattac cgctatgaat gcatttaatt ctggtactgt tggtggagct
      421 gatggaagaa tagggggagc acaaaatgtg tcgtatttta tctctgatgg taatccaaca
      481 gttaatcaag attggccaag tattccaggt acattagcac aaaatgggat tcagccaagt
      541 gagcaagctg catgggaagc tttcctgcaa gacacgaatg gtgcatctgc tggtggcgaa
      601 aaaattagat cttatgcctt aggcatgggt acggggatta ctgtcagcaa tcttgagcct
      661 ataggattta atggggtaaa tggtacagat attcctgctg ttgtcgttac agatattagt
      721 caactagaca gtgtattagc aagtacaatt actgcaagcc caattagcgg tactttgatc
      781 tcggatccta accctggtgt taactttgga gctgatggag ggcacgttca atctgttaca
      841 gtgaatggta taacttatac tgttaatgca aatcttagtt ctctttctac aagtggttct
      901 gctactcaag gatcaaatac tcattcctac gataattcaa cgcatgtgtt aacagtaaca
      961 attagcacgg gtgaatcgct tactatagac atggataatg gtagttatac atttacacca
     1021 ccagcaaata ttaccagctc aataaaccgc ataatcaact atgttctagt tgatggtgat
     1081 ggtgatacgg ctgctgcgac aattactttt aacattgata tttccggatt caatgatccg
     1141 ttggtagtac gtgatgattt agttcttacc aatcaacccg ctgtaagtgg tcaagatgtc
     1201 atttctatac cgacatgggc attattagcc aatgatacgg gtggaagtgg cgtgtcttca
     1261 gtcagcagtg tgacacaacc agttgctgaa ggtactgtag ttctcaatgc tggttctgta
     1321 gcttttaccg aagaatctaa tagtgcagcc gatggtacct acaatggaag tacatctgat
     1381 ccttttaggt acactaatac gacaggttct caatcagcaa ccgctaaggt tgatctggat
     1441 cgtacacaag caggggaaaa taggttagat ggaacctaca gaagcgagat tttattaggt
     1501 agagatactt cgaatgacac aattattggt aatgatggta atgatatact gattggatta
     1561 ggtgggaatg atactttaag tggtggtaat ggtgatgata ttcttgctgg tggcgctggt
     1621 aatgatacgt tagatggtgg tgcaggcaat gatacagcta cttacattga cgccactgca
     1681 ggtgtaactg tttccttaaa taccagttct gcacaaaata ctgttggtgc aggaacggat
     1741 acacttacca atatagaaaa tctaaccggt tccaaattta atgatactct aactggaaat
     1801 aatagtgcca atacaatcaa aggattggag ggtaatgata caataagtgg attaggtgga
     1861 aatgatactt tagatggcgg ttccggagac gatgtcatta ttggtggatt gggtgatgat
     1921 atactcaccg gcggatcagg ggctgatcaa tttgtgcttg tgaaagggca aggtggtgct
     1981 agtgcaggcg cagccccaat cgatactatt acagactttg aagtgaacat tgataagatt
     2041 gttattaacg gtaataacat tactggtgtc agtgtatcca ctccggtttc taacacttat
     2101 actataacgg ttaattattc tgatgctgct acggaacact ttaaagtgac attatccaat
     2161 ggcgctgttt tgaatgattc aggaaaaact catttgaacg gagtggttat aagcggcggc
     2221 accgctacca ttgatggcac aatcgttggc gcagttctat atttggatct gaatgctaat
     2281 aatcaggagg atgaaggtga gcgacttggt attaccaatc aatatggtca tgtagaatgg
     2341 gtcgttgatc tgagtaaatt cgatgtcaac ggtgatggtc agtatgttat aggcgaggct
     2401 cgtgctgttc aaaccggtgg ttttgatata gatacaggtc ttagctatga aataaatctt
     2461 tacgggccag taggttcagc agttatatcg cccttgacaa gtctcttgca agcgcagcta
     2521 gaagccggta tggattatga gacagctaat gctgcgttgg ttgctcgctt agggttacca
     2581 gagggaacac aaattattag tttcaatccg attactggta gtggagaagt cctggctcaa
     2641 aacgcaggtg taatgacagc tgccattcag tttgctgaaa tagccgcaat acactattca
     2701 accgatgaag gtcatgtttc attcgctgtc tttgaggcaa ttagcaaggc attgcttgaa
     2761 cttcctgaag gggtagtcgc tgattttggc gatgagggtt tcttgcagtc catttcaaat
     2821 tatctgggat tagatgctct ggttacatct gatatgattg actatatggc aaccagccag
     2881 aaagcgttag aggccagtat tttgactctt gcgcccggag aagatgctct gacggccatc
     2941 agcaagatac agcatgtgac ccaaggtata tatgcccagg ctattgaaat ggcattgata
     3001 gggtatttgc cattagatgc tcttaaaaac atgagtgctg ttcttaaggc ttatgctgat
     3061 ggagatatta cggatgaaca actcaactcc tttgaagata agctttctgc tgtgattgtg
     3121 aattgggaag ataacaatgt tacagacaca tctcatcagg ctacattgca aacagagcgt
     3181 actgttgatg actcttcagt caaatcagag gtgaatcatg aaatggcgca agaagttaat
     3241 gccgtgatta atgattttat ggatgaacat aatatttctt tgtactatta tgatcaacag
     3301 gctcaaatgc ctcaagaagc acaacaacca acagaggatc aggctgaaga gacggtcatg
     3361 catgacgacg ttgttcaaga tttgttgctt gatgatcata gccttaccat gttggctaat
     3421 aatcccgaat taaatggtgg taatggcaat gatattctgc atggcactac cggaaacgat
     3481 tttataagag gtggtcaagg aaatgatacg atgacaggtg gtggcggtgt tgataccttc
     3541 ttctggttaa gtggcgatga tgatggtggt gtggatacta ttactgattt caaagcaaat
     3601 ccagttgata agtcatccga tgcgagcgtc cttaacttaa gtgatttgct atctgatgct
     3661 gatctggaaa ccaatagttt ggataattat ctgaatgttt ctaccacaga agagggtgac
     3721 acagccatta aagtagaccc taatggtaat ggaaactttg acgctccagc gcaaacgatt
     3781 atattagaag acgtggattt aacagctgtt ttcgctacca acaattcgca tgatattgtg
     3841 aatcaaatga tcgctaatgg aaacttgata gtagaacagt aaggtgaact cattacattg
     3901 gtgaatatgg aattcaccaa tgttttatta attggaaaat atcctcgcga catttttctc
     3961 gctgctatat actcatgcgt tcattcgata ataagccttg caattcattt cacgttatta
     4021 aaatgacgaa aaaaggcaaa aaacgctatg gagcagtatc atgtctaatc cttatatctt
     4081 ggttttatac tattctcgca ctggctcaac ggcacaatta gctcaatata ttgctcgtgg
     4141 ggtggaaagt gtgtctgggc ttgaagccag gttacgcact gtacctcctg tatctaccac
     4201 ttgcgaagcg gtggataagc cggttcccga tgctggtgca acttatgttt ccttagatga
     4261 tttgcgtcat tgtcatgggt tggctttagg cagtcctact cgctttggta atatggctgc
     4321 tccaatgaag tatttttggg atggaactac ttctttgtgg ctgggtggag atctggtgaa
     4381 caagcctgct tgtgtgtttt cttcttcagc cagtatgcat ggtggacagg agactacctt
     4441 aatctccatg atgattcctt tgttgcatca tggcatgatt ttattgggtg ttccttatac
     4501 tgaacccgcc ctaaccagta cgcagtctgg tggtacgcct tatggtgtaa cccatgtggc
     4561 tggtggtgat aatgagaatc cattaagtca agatgagatt tcgttggcta aagctctggg
     4621 ggaaagattg gggcgtgtag caatgggact gaaggtttga tgagagggtt ttaggggttg
     4681 attacccctc tcccttaccc tctccccaaa ggggcgaggg aatgattaat taaactatat
     4741 cttctctcca aataggcgag ggaatgatta attaaactat atcttttctc caaataggcg
     4801 agggaatgat taattaaact atatcttttc tccaaatagg cgagggaatg attaattaaa
     4861 ctatatcttc tctccaaata gatgagggaa tgattaatta aaactagagt tttctaaatt
     4921 gatggctatg cgagttcatt gattttagag tgataaattt tttgtttatg agcaatgatg
     4981 taatttggta tttttaaaga aaattgtagt aattggcgct gatatttaaa caaaaactcc
     5041 ctctcccgcg aaagaaattg tataaaaaat ttgggtatga ttcgcgggag aggggtgggg
     5101 agaggggaat aagtggttcc agctgatgct aaattattga aacaagcgaa atctctccgg
     5161 aaaaggagca ctgatgttga aaattgttta tggtactatc taagagcaca tcgattaaaa
     5221 ggatacaaat ttaaaagaca agttcctatt gggaattata ttgtcgattt tgtttgtatg
     5281 cagagaaaac tcattgttga actggatggt ggacaacatc ttttaaataa agagtatgat
     5341 aaagaccgga gcgcctattt tagcacatta agttataagg ttttgcggtt ttggaatgat
     5401 gacgtgttat tgagaactgg agacgtactt gagagtattg tcagatgctt ggaagaatca
     5461 taaggttgat aagattaaca tttaactgtt cagtaatgat agcggagtag cctaagtaat
     5521 gaaagtaatt agttttaacg ccaatggcat acgctcttct gcccgtaatg gattttatga
     5581 ttggcttgta gggcaggatg ttgattttgt atgtattcag gaaacgaaat cgcaagtaga
     5641 gcaattaatc ccggaagaac tttattttcc aagggattat ttttgtgatt attttgatgc
     5701 tgaaaagaag ggctacagtg gggttgccat ttattcaaaa tataaacccg tgcgtattgt
     5761 gaaggggttg gggtttgatt attgtgataa agaagggcgt tatatccaat ttgattaccc
     5821 taaattaagt gtggtttctc tttatttgcc ctctggaaca agtggtgagg atcggcaaga
     5881 agtgaaatat gcttttctgg agcaatttgc cagacatttg atgaatttaa aaaatgaggg
     5941 aagagaatta attatttgtg gtgattacaa catcgcgcat aaaaagatag atttaaaaaa
     6001 ttggcgaggg aaccagaaaa actcgggatt tttacccgaa gaacgtgcct ggatggatga
     6061 gttatttggt tctatggggt ttgtagatgc ttttagggta cataaccagg aagaagagca
     6121 atatacctgg tggtcttatc gagctagagc ctgggaaaag aatgtcgggt ggcggattga
     6181 ttatcaggtg attacgcctg gtctgactga atatgtcgcg gacagccgaa tttttcgcga
     6241 agcacgtttt tcggatcatg cccctttaat gattgaatac aagggagact ggtgtgttta
     6301 aactcgctag ctggaatgtt aattcattaa aagtccgtct ggaacaggtt ttgcaatggc
     6361 ttgagtcttc acaagttgat gtgcttgcca tgcaggagac taagctgacc gatgagaact
     6421 ttcctgtggc ttcatttaca gacaaagggt accacgtcat tttttcaggt cagaaaactt
     6481 ataacggggt tgccattatt agtcgatatc ttatgacaga tattcaaacc gatattccag
     6541 aattggaaga tccccagcgg cgtatactgg ctgctacggt aaaaggaatc agattgatta
     6601 atctttatgt gcctaatggt ggagaactga cttcagacaa gtaccaatat aaactggatt
     6661 ggttaaataa agtgactcgt ttcattcaac atgagataag catttattct aaagtagcag
     6721 ttgtgggtga ttttaatatt gcgcctgaag atcgggatgt ccatgatcca gttgagtggg
     6781 aaggttgtgt tttggtcagc cctcctgaaa ggcaagcatt tatgaagata cttgaattgg
     6841 gtttacatga cagtttccgt aattttgctc aggaagaaaa agtgtttagc tggtgggatt
     6901 atagagccgc agcattccga cgtaatcgtg gtttgcgcat tgatcatgtg ctattgagcc
     6961 atgaattaaa tgctttatgt cgacaatcca aaatcgataa agaaccaaga aaagcagaga
     7021 ggccttccga tcatgcgccg gtttgggttg agttagaatt ataaatgtct gaggtgtttg
     7081 agaaaaaatt gctttatgag atcatttctg ctattgtttt gactcatttg tgtagagtat
     7141 ggtgcttggc aatagcgtcc aggtggcgat acttatttga aactagaaga gagtggtatt
     7201 atgaaagcat cagttcatcc tgattatcaa acagttaaag taacatgcag ctgtggtgaa
     7261 gtatttgaaa ctcgttctac tttgtgtaaa gatttgaaca tagaagtttg ttcaatgtgc
     7321 catccttttt atacaggcaa acaaaagttg gttgatactg gtggccgtgt tcagaaattc
     7381 cgtgacaggt ataatatgcg tactggacaa gccaaatcaa aagaataata ttttaatttt
     7441 tgagttttta acaaaggcac aaattttttg tgccttttct ctaagagagg gttaaaaatt
     7501 caaatttttg atatgatgtt ttcaccgcgt gatctgaaat agagagtatg cactttggat
     7561 aatgaagtaa taaagcagcg ggcattggat tatcatgagt tccctgttcc agggaaactc
     7621 tctgttcatc ttacaaaatc gactaattcc caagacgatt tgtctttggc ctatacaccg
     7681 ggtgtagctg ctccagtttt agccatagct gaagcgcctg aaaatgctta tcgttttacc
     7741 agcaaaggca atctggttgc ggtgatgacc aatggtactg cagtgttagg tttgggaaat
     7801 cttgggcctt tagcgagtaa gcctgtaatg gaaggtaaag cggtattatt taaacgattt
     7861 gccgatcttg atgtctttga tatagaaatc gatgcagagg atcctcagtc ttttattgct
     7921 actgcaaaac gcattgcccc aacttttggc ggtataaatc tggaagacat caaagcgcca
     7981 gagtgttttg aaatagaaca agcattgatt cagcagttaa atattcctgt atttcatgat
     8041 gatcaacatg gtactgcaat tgtagttgca gcaggtttat taaatgcgtt ggaactacag
     8101 aacaagaaat taagtgacat taaaattgtt tgtatgggcg ctggggctgc tggtattgct
     8161 tctatgcgac ttcttgtggc attaggagct ccaaaaagta atatgctgct gctagatacc
     8221 aagggagtta ttcatgatgg ccgcgaggat ttaaacccct ataaatttgc ttttgccaga
     8281 aatactccct gtcgtacact tgaagatgct ttggtcgatg ccgatgtgtt tattggcgtt
     8341 gcaaagccag atttgctgaa tgctcattta ttaagtttga tggcgcccaa tccagtgatt
     8401 tttgctttat ctaaccctga tcctgaaatc aaacccgaat tggctcatag tgtgcgtaaa
     8461 gatctggtga tggcaactgg acgaagtgat tatcctaatc aagttaataa tgtattatgt
     8521 tttccttata tttttcgtgg tgcgctagat gttcgtgcca cttgcattaa ccaggccatg
     8581 caaattgcag ctgttgaagc cattcgtcaa ttggtgcatg agcctgttcc tcaggtagtg
     8641 aaggacaatt acccaggagt tgtgaattgg gattttggac ctgactatat tattccaaag
     8701 cctattgatc ctcgcttaaa agagagggtt cctgctgctg tagcgaaagc tgcaatagcc
     8761 agtggcgcga gtcaatttaa taatttataa ggcagtaaat tcaccttata gaagtaagca
     8821 gttgaggtct taatagcgtt taaacgaagg atatctttga aagttctttg gaaaccatca
     8881 gttggttttc tggaaggttg caagatgggc ttttttgcgc ttacaagata cgctgggatg
     8941 cataaatggg aactaagatg ggatttaaga atacttgact aaattgttaa ggaggtaatt
     9001 ttgcatactg aaaaccgaac taactatgca ttgataggat ggctactttg tgggctggga
     9061 gcaatatttt atagttatga gtatttgtta cgcatcgcac caagcgtaat ggaagacgct
     9121 ttacgatctc actttaattt atcagcatct ggttttggtt ttctgtcatc tttttattat
     9181 tttgcttatg ttcctatgca attgccagtt ggagttttac tggacaggta tgggccaaaa
     9241 aggctattaa ctctggcgtg tttagtatgc gttattggaa cgtatatgtt tacggggaca
     9301 actatttttt gggtagccgc aacaggtcgt tttttagttg gatttggttc tgctttcgct
     9361 ttcgtcggtg tgttaaagat cgcaactctt tggctgcctg aaaatcgatt ggctatgatc
     9421 tctggtatga cgtcggcact aggccccatc ggtgctatgc tgggcgataa ctttctggag
     9481 ttattcatcg ttaagatggg ttgggttaag acacttaaca tgactgcagc ttttggtatt
     9541 gtattgactt tagttttgtg ggttgggatc agggatagga agggacagca caggcaaagc
     9601 ggtacagttc ccactcttaa gaaagggtta gttgatctgg gaattattat taccagccga
     9661 cagatatggg ttaatggaat gtatggctgt ttagtttatc tgccaactac ggtttttgca
     9721 gagttgtggg gtattcccta tttacaccat gcccatggtt taactactca agcggcagga
     9781 ttagctaact ctgtactatt tttgggattt attattggtg ctccaataat gggatatatt
     9841 tcggatcgtt tgtatcgacg taaattccca atgctaattg gtgctagcgg cgctgcaatt
     9901 gtcatgatga tgattttata tttgcctgga ttaaatgaat ccaatattca agctttaatg
     9961 tttttattag gattgcttta tagcgctcaa gctatagttt ttgctgtggg cagagagttg
    10021 agtcctggtg aggcagcagg aacggcaatg gcggtaacta atatgatagt catgctcggc
    10081 gcaatgtttt tgcagccgtt ggtagggcat ttattagact tcagcttatc aactcattta
    10141 agtgatacta ctggtattgt tgtagataac atgagtaaac tatataccgt agatgattat
    10201 cagtttgcct tatccattat tccattaggt atattgatag cggccatttt aactttcttt
    10261 ctaaaagaaa ctcatgcaaa agcacctaaa taatatgact actaaaaaac aaatttcata
    10321 tggcgtccta gtttgcttta tcggggctgt tttttactgt tatgaattta ttctcagaat
    10381 tgttccggga gctttacaaa cagagctaag tacagctttg ggacatattt ctgctaccac
    10441 ctttggacaa atttcagccc tgtattattt tgcatattca cccatgcaaa tgcctgtagg
    10501 gatgttaatg gatcgctatg ggccgagacg acttttgact tttgcctgtt tgtgttgtgc
    10561 cttgggctcg tggatgttta ctttgacctg gtcaattgtg ctggttggaa ttggtcgatt
    10621 cctggtggga tttggctcat ccttcgcttt tgttggtgtc ttatctctgg ctttgcattg
    10681 gctgccacgt cgttattttt ctttagtggc tggtttgatt accacgctag gtatgctagg
    10741 actggtttat ggtgaaatca aaataactga atggtcagag gtcataggct gggatcatgt
    10801 tttaatgcac attgcaatga ttggttctgc gctgtgtatc ttaactttct ttgttgttag
    10861 agatggtcca gaaggatatc aacccaacaa atatccctgg cccgagtttt tccataacgt
    10921 gcttaaggta ctgacgtccc atgaagtgtg gcttattggg tttgttggag cctgccttta
    10981 tacttcatta tcagtatttg gagagctgtg gggtaaaacc tatttggagc aagcacatca
    11041 tttaaccaaa gtagaagccg caaaaactgt ttctgctgtt tttcttgggt gggcggtagg
    11101 agcccccttg gctgggtaca tatccgatat tacccgcaga cgattgttgc ctttggtgat
    11161 tggtgcgata gtttcattaa tatgcattag ttttgttttg tattgccctg gcctatccta
    11221 tcttaccttg aacatattgc tattcttgta tggtgttttc agtgcaactg aaattatagt
    11281 ttttattatg gctaaggaat gtagtggtgc caaattatca ggaacggttt ttgcagtaac
    11341 caatatgatt atttctttgg cgggtgctat ttttcaaccg ctcgtaggga agttactgga
    11401 tacatttggc gacagcggta tagtggcagg ggaacatatc tatacggttg atgactatca
    11461 agtggctctt tcgatattac ctatttcatt acttttggta actattctgg ctttctttat
    11521 taaagacaaa gaccattcca ataactctta atcttgtgat aaagactctt ctttgtcttt
    11581 gtcaggatgg acagttgaat actgcgacta gctctttaac cggcagtcgc atctgacttc
    11641 tgcagcttat ccagcgaggt acatagaaac tctttatttg tgcttttttg taatgatgtc
    11701 gcgtaaattc agtatcatga ttcgatatga tgacaggaat ccctttggag gctgtttcct
    11761 gggccaattc agccaactca atttgatctt ctaaagaaaa taatttttgg ttatagggta
    11821 atgtttgatt gttttcggtc agtggcacat agggtgggtc acagtaaatg acatcaccgg
    11881 gttcagctaa ggcaaaagtg tttctaaaat cgtcgtgaat gaactgtgcc gaaaggcatt
    11941 ttttataaaa cagcatcatt tccttacgtg gaaaataagg tttttcgtaa agaccaaaag
    12001 ggacattata ttttcctttt gaattgtatc ggcacaaacc attatatcca tgccgattaa
    12061 gatagagaaa aaccgctgat ttttgatttg aatccattaa attattaaat tcagttcgca
    12121 attcataata tttttctttg caattggctt cgggggtaaa gtattgctgg caatagtcta
    12181 taaaggattc gccattcaat tgtagaattc tataaatatt aaccaaatca ggattgcttt
    12241 cagccagtag gtaagatgaa tagctggtgt tcataaacac cacgcctgaa cctgcaaatg
    12301 gctctattaa acgttggcca gtgggtaaag aagagataat ttcatttaaa caattgtact
    12361 tacttcctgc ccatttcaga aaaggtctga tttttaccat aacttataaa atcaatattt
    12421 taggatgaag tatctcttat tttcaggata aagaacagga taatgaataa aggttcctta
    12481 tctgatttac aaaatagccc ttgtactggc ttcgcattac tttaaaatat atcaaatttg
    12541 tactttttaa cgctgatatt cgggctttaa ttaatacatg atcgcaatga taagcaaacc
    12601 aatggcgata aatcctataa ggcttaaagc tggatttcct ccaggaatca aatgagtttc
    12661 tgatggagat tggctacagg tttgtcttcc tttccatgcc atgatagcag gtaataacag
    12721 cagtaaaatg acacaacaaa ccccggcata acttaatgca tgcaggtaga tacctgggtt
    12781 aaacaacacc acggccaatg gagggataaa agtgagagcc agcgtatact tgccttgatt
    12841 accagatttt ttaagcttta atccatcagc aagaaaatca aataatccta atgaaactcc
    12901 cagaaatgca gtaaccatac agatagaagt gaaaaaccca aaaaatccac tgatccactg
    12961 gctgcttact gattgactaa gtgcttcagt gaggccgctg gttgcatgat ctgagttcat
    13021 tagggacaat aaaccatttt ctccctcgcg tgagataacg cccataatca ctgcatccca
    13081 gactatgtag cagaataatg ggataagaga accaaacaaa ataactcgac gtaagctacg
    13141 tatatcatca ttaaaataat cacgtaagct gggcacgatg gatgcaaatc caaaagaggt
    13201 gaccaagatc atgagagtgc cagtaaaggc tttcgctgag ccgcctgtta atcctgctgt
    13261 ggatacatgg ggactgataa taataaccaa taaaatataa acgcccagtt tgccaaacat
    13321 aagtccacga ttaacgtagt ctacagagcg tataccacta tatacaataa ggctgaacat
    13381 ggcagtaaat attacagacg ttatccaatt cggtaaatca atgtgggcct tatgcagcag
    13441 gctgctaaat acatcacttc cacctgaaat ataagccgcc aggagggtat aaagaagaag
    13501 aaggtaggtt atccaggcaa ttacctgacc tggcaggccc aaggttgatt tcgccattga
    13561 aatcatgttg cttcctgccg ggagtcgtag attgacctca agaattaata aagctccagc
    13621 ggtcatcacc aaccagcaaa aaaacaggaa aaataatgag ttagtaaatc ccacctcggc
    13681 tgtagaaaca ggtagcgcta gcatcccgcc accaatcgaa gtgcctacta tgagtaatat
    13741 accaccaatt aattttgaat tgatcacgaa gtataaccca aaaaaacagt acaaatacag
    13801 aacaaaatga tactttgttc ctcaaaaaaa gtcaacttcc ttattgtaga cctcaatcat
    13861 tttcttttca caaatgacct ggcgttcaat ttatacatcc tgtttatcag gttttaatgt
    13921 tttactgcta tcaaagtaaa aataacgcca gtaataacat taatcattct tattcaaaaa
    13981 gcgcaataaa ttcatgtgcc aataacacta cagaaataat aatcactgcg aactgtgata
    14041 ttttacctcc tggaacccaa aagggtgatt tgtaacgctt tctgccaaaa taacacatta
    14101 gggctggcaa taacagtagt aatatgatgc aaaatatacc ggcgtagcgc agagcataaa
    14161 tataagcccc tggataataa ataacgatta gtaaaggcgg caaaaaagtc aaaacaaata
    14221 aaccaattcc atggcggcct cgctctcgca attttaagcc atcagcaaga aaactaaata
    14281 aacaaagtga tacacctaag aaggcagtta acatgcaaat ggaggtaaaa aaattaaaga
    14341 gggcgctgat tgtggtattt tcaactttat cagttaacaa actggctaac gcgctggttg
    14401 tacgcggatt ttgcattaga ctttcaagtc cgctagtgcc ttctgaaggg agtgaaccaa
    14461 taattacggc atcccaggct atgtaacata tcaaaggtat taatgaacca ataaaaataa
    14521 ctttttttaa tgtcttaatg tcatcatcaa aataatctct cagatttggc acgataatag
    14581 caaaaccaaa ggaagtaatt aaaatcataa ttgcgccacc aatttgtcga taatcaccat
    14641 gttgaaaata gtgaaattcg atgtgtggag caatcaggat aacaagtatg cagtaaacag
    14701 ctaatttacc gaacatgaga gcgcggttag caacatcgac ggaatggatg ccaccatata
    14761 ccaccaggcc aaaaattaaa gtaaataacg aacttgcctg ccaatcactg agatgccaat
    14821 tgactttgga caatagactg ttcaatacat cagcgccgcc ggaaatgtaa gcggaaagca
    14881 aggtatacaa taagaaaagg taactaatcc aggcagtaag taacccataa tcgcccagag
    14941 ttgccttggc catggaaacc atgtgttttc ctctggacaa atagagattt gcttccagga
    15001 tgaaaaaggc accaaaggtc ataaagaccc agcagataag aagaaacaga gaagattgcc
    15061 aaaaaccggt tgctgcatta gctactggta gagccaacat ccctccacca atggaggtac
    15121 caactatcaa taagatgccg cctataaatc ttgaacgcat taataaacct ttattttaca
    15181 taagccattt gtggttgtgg gggttgagca gggcttgtaa accactggat ctcgattctt
    15241 ctgttctgag cgcttccatg aataatagca ttgtcagaaa tcgcattctt atctccatag
    15301 ccttctgctt ttagtctttt ggctgcaata ccattagccc agagaaaagt catcattgtt
    15361 tcagcttgag cttgagaaag ttttctctta tggctgcgtg atccgacatt atcagtaaat
    15421 ccggcaacat aaatcgtact ttgaggataa aaatttaaca gtctgatgac attgtttagg
    15481 ccaggatagc atatttcatt aaggcgaggg ctgctaaaca tgaaatattt gtctgtagga
    15541 ataattaatg ttctggtatc tccatattca acatactgaa tatcctgctt ttgcagatct
    15601 ctgatgattt tgcgtttgct gtcacggtag atactcgcaa ccagacctac ggtaccacca
    15661 gccgctgccc caataagagt tcctgaagca gtaccactgg ctattacccc agctacagca
    15721 ccaatccctg tatctactcc tactctctta acagctcgtc tatcgggttg aaagttgtta
    15781 taagggggat gaaagcagcc cgcaagaaag caaatcatta atccataacc aaggattcgt
    15841 gttttggcta atttgcgtga catatccact cctttaatca atgatttaat cattattaag
    15901 catctcatta aatttcgcaa tctaacactt tatattttta caggacgtat aaaaaaataa
    15961 ttttcttcgt ctataattga aatagtcaag gaagaaacag agtgatgcac tgtgaaagga
    16021 aattcaatgt gcccactccc ccaaatgcaa ggagcggtat gcctggctca ttacccgggt
    16081 taccgaaaaa gctatgaaaa ttccagtcca actcaaaaat ataggcggta atatcccaga
    16141 aaatgatatt gctttgcaag tttcttattt tattaaatct caaaatgacg aattgcttcc
    16201 ccaaagtgac gcttatattg ctccagcttc aggcaagatt gatgtcagct ttggaaataa
    16261 tctcgcgcga aataaagatg ttttctttgc tgttgcatta aaaagcaaca atattttggc
    16321 tcaaacttct acatctgcta ctcaactaaa ggacgaagta cctatcacga tggagtttga
    16381 ttacagtcca ctcgtattat cagaacctga cgatcaaatt gtttctccca ggccattatt
    16441 tgtttatggg cgcttgcttg ataaacaggg caagaaaaaa ctggaagatg ttcaaatcat
    16501 ttttgaggct actcgtgcta atgatggagg gttacagccc atcgcaagcg taagaacaga
    16561 agcagatggt tattttttta ttgagtatcc gcaaggtagt tttattgatg ccagtgcccg
    16621 agtgggtttg ccccttcgtg aaaacccaat ccctgtccgg cttgatgaag ttaggattga
    16681 tggcgacatt tcgaataaag tatttcctcg caatgttatt ttagttgcgg aattaatgga
    16741 agctgaagaa gcaactgtcg agaaggaatg tggctgcgag gtactggatg ttcatgagag
    16801 taaaagaatt ttggaagaat attcctttta tagtgtggtg cgcaccactg aacctgaaat
    16861 attgggttat gtcttaaaag aagaagatga gattactttg gatgaagtat taacccagca
    16921 tcctatccgt atttgggaga ttatcgatcc tatttttcaa ttacctgcat ttagtcatct
    16981 tgcaaatcgt gcaagagtaa acctgccaga agtcacagaa agaagcatca atacggaaat
    17041 aacaacaaga gcgcatggcc gcgcaaatac cgcacctaat ccgccccaag acaatgaact
    17101 ggctgaaact ttaaaacaga ttaaaattaa tcgcggtgtt ttaaagaatt ttttacaaag
    17161 agaaaccagt atcaccaaag ataacattgt caatctcatt gaaatgaatg aaagttatcg
    17221 ctttaagcaa aaaatgactt tgggagaaac aaaaccgctg ggaagagtcg tattgaattc
    17281 cgagaattcc gtggattggg atttagaaac tactctatat caggccgtat cagttgctca
    17341 tgggcattta ctgcatttta agtcagagtg gatcgcagat ggttattctc ttggagatct
    17401 cctttatagc ttaccattgg caccaggcca gaagaaacag attgtagtat ttgattggga
    17461 acgaagagag tcagctacca atatacaatc tttagagtat gaagaaagtt tatacaactc
    17521 acttagccgc gatagagata tctttgaaat cacaaaagga gtgattgaag aaaacataaa
    17581 aggcaaatcg agcgcaacta ccgcaagtgc cagcgcaggc attggaggag ttgtgggagg
    17641 tttgcttttc ggggtttcag gtggagttgg ccattctggc tcgactgcct ctcaaaatag
    17701 tctaaggcaa acctccgcaa gtgatttgca aaaaattcga gatcgcattg tacaatcagc
    17761 caatgcggtt cgtagccagc gctcttcagt aattcaaacg gtttcccagg gtgagcgttt
    17821 tgaagtgtcg tctgaaacag tggctaacta taatcattgc catgccatta cgattcagta
    17881 ctatgaggta ttacgtcatt ttaaagtgcg tcagcgtttt gcagaagcac gcgaatgctt
    17941 gtttgttcca cttcttatga gtcaatttga gttaaataaa ttattacgtt ggcgagaaag
    18001 tcttcaattt gccttattga atactaattt gtcaaagggg tttgacgctg cagaaagaat
    18061 taaaaataaa tggatcggta gcaattttcc aggaggaacc ttcgcttccg agaaaattct
    18121 taatgcatca ggcaacttcc aaatcaaatt tattatccgt agacctgatg acaagatggt
    18181 tgaagtggat gattattcaa agcctatcta tggtggccag aatatgatta tcggatatca
    18241 gaaaaaaaca gtcgaggata tcaacgaagc gaattggcaa agtttaattc cttttctggg
    18301 atcagatact ccgaaaggtt tttatgatca tcatttaaga gatgcgaaaa acaaggatga
    18361 agtatttcac cgattgcttg gagagaaaat tgcaacggca tttgttggtg ctctcacgtt
    18421 tcatgttgcg gatgagacag gtaatgaaat ttcctcaatc tcctttgata cgactctgac
    18481 ttcaaaatat agaaaagaag gtgttttaaa tgtttctgtg cggtttatgg gaccttcaca
    18541 atttagcaga gacaaagtgc attatattaa aattcgttgt ggtacgacca atgtgttacc
    18601 cgattattcg tcaattattg tcacttcggg atttataaga tacaagacgc ctcactatga
    18661 aggcttttta tgtcgtttcc aaagtattta tgacgattta tctcctagtg atggtgtaac
    18721 gctatatgct ggtccttcac tcgatgagtt acgtgatcca agaaaagaag atattgctct
    18781 ggtgaattcg ctgattgatc atttgaacga taatttggag tggtatcaca aagcgatctg
    18841 gcaactgatg cctgccgaaa gaagattttt actcctggat ggtatcgtat tacccggtag
    18901 taaaggtttg ggaagaagtt tggcatctct ggtggaaaat gagctaattg gtattgtagg
    18961 taatagcctg gtttttcctg tggcaaaagg attgaatctg gatcctaatt ttggtgcttc
    19021 ggattctctg actgattact atatggtggc tgctggtgat cctgtcaatg tttcaatccc
    19081 aacgaaaggt gtgtatgccg aggcgatgat gggacgttgc aattcttgcg aagaaaaaga
    19141 tgaaagtcgc ttctggcgtt gggaagaatc tcctatacct gacactccaa caacaatcaa
    19201 tccaatcagt accgagagtc gaagagcgga acccggtaac ttgcaaccga ctgcttttcc
    19261 caatcccatg gttaatattc aaaatgcacc gaatgcccct gatcccacag gtttggcggg
    19321 aacactttct ttattgggaa aaggagattc attcagagat attacgggac ttactcaaaa
    19381 tcaaaccaat gcgttggagg ccttgaaagc atcctttgat gcgaccaaaa catttgggca
    19441 agaggcggcg aaattggaaa ttcaaaaaat gatggataaa cgattagaca atgcgattaa
    19501 agcgattaat aacaatccaa atctggatcc aaaacaaaaa atggagctca cagaaaaggc
    19561 gttaaatgct tatcttggcg caggagccaa tacaacgcaa ccgccgaaag atgccaatgc
    19621 cgacaagttg caggaaggca ttaataaaaa cctggataaa gtgaacagca gcaagagcgg
    19681 cgagatatct gtaacaaaac cagatggaac taaagtcaat acaaaatttg acgggggtac
    19741 cacgtctcca gctattgaaa ccaaaatagg taaaggcggc attactccta tgaagcaaga
    19801 aaacagcaat gcctgttggg caacagtcgc taccatgatg ttgcgatgga aagacagtaa
    19861 gaattataca attgaagagg ttttaacgaa agctggatct caatatctta actattacaa
    19921 acaaaagatt ggcttgccat atactgaaaa acagaatttt attacttctt taggtatgaa
    19981 aggagaaccc ttagccaatt atacggcaca aaattaccgt gattggttag ttgagtatgg
    20041 gccgttatgg gtcacaacag atactgatca aagcaaaggt ttttcagcac atgcgttgat
    20101 tattactggt attagtagcg acttaaaatc tttagaggtt atcgatccct tacaaggcag
    20161 aaagtttgtt caatcgttcg aggaatttgc caatgcattt aaggagctta ttactgacag
    20221 tggtctttta ccaacaacac aagtagttca tttcctggag aaaattgagg caacagaagg
    20281 agcgccaact cctgctgata ttgttaaaag tttaaatgaa tacaatccct ccagctcaga
    20341 aaccctagta attaatatgg ttgaattcaa tacgggtatt tcagggatca ccaattacaa
    20401 agattggaaa acagacggtt cagtgataca caacaagcgt aaaaatgctt acagagatcc
    20461 ttttggtatt aagcaccttg tgcttcacga aacagcggct gaaagcggtg atgggtttga
    20521 tgattccaat aatgaaacct cacatatgtc ggttaaaaga gatgcaacca ttttacaatt
    20581 taatgacctg gtcgagtttg aaaatcatgg ttcggggatg aacacgacca gtattggcat
    20641 agagtttgta aatcgcggat ggctatcttc ttctacagcc gatggcggtg aagggatacc
    20701 tgcgaaagaa tccagcctga ctgcagcaca aaaagaaacc tacaaagagg ctaatgggta
    20761 tttatgggca ttttggggat atggttttaa catttatcgt gttcccccgt ctattgatca
    20821 gttagaaaaa gaggtggaac ttgtgaagtg gctcaccaga gattttcctg ccttattgca
    20881 atccatcagc ggtatttcta tttataacct tttcccctca attgatgata cttggttaca
    20941 gcttgtctct tatgctgagg tgaaagatat atggactttc aaagcagcag acataccccc
    21001 tgaggcagaa aggactgaga agaatctctt tgtcatgaca acaggatatg agtatctgga
    21061 acccagttac ttaacagata agagtggaat tatttcacat aatgcctttt atgagaatca
    21121 cagtgatggt tctttcttga cattatatac ttggttgcgt ttggagaaag gaaaatcaaa
    21181 aacagatgcg ttggatattg ccaagaaact gatgaaggat catttcattc gtgtttcttt
    21241 aacgagtaat acagacaaga aaataatttt gctgaatgtc aaggatggta atttagtttg
    21301 atatgggcat ccaaaatcag gtaatgcaac tctatatata tttgagatac accttttagg
    21361 ggtagagtat ctgtgtaaat ttggctccat tccctaacgg ccgtggctct gattattaat
    21421 cagtatcaac ttgctctatg tccccgattt aaaactattt taaaataata aattatggca
    21481 ttatgacttt ctatttatgc tttattgata agaacaaaac ttacgaaaaa aaaccaaggt
    21541 ccaggcaaaa aatgtttgta atgagggagt ttagatagac taaatgaccg cattgaaaac
    21601 atttttaaca aagagattgg agtttttcat aagtcctgaa gaaaatataa tgcgaacaaa
    21661 gggttagtac gccaaagaat tcatggagaa ctatatgagt aaaaaagcga tagcaataag
    21721 tatcgtcatc gcactttttt tcatcacagc aatttggttt gcgtacagct ttgttaaaca
    21781 ggaagaaaat caaaattggc ttactcttta cggaaatatt gatatacgtg acgtttcatt
    21841 atcctttcgt gtctcggggc gtatcctgtc tatggacgtt gatgaaggtg ctcatgttaa
    21901 gaaggggcaa gtactcgcca ggcttgataa agatactttt atagccaatc tggaaatggc
    21961 gaaagcccaa ttggagcaag ccattgccaa tcaaaaaaat gcaagcagaa cttataaaag
    22021 acgtgagcaa ttagttgatg acggggctgt ttcaaaagcg ctttttgatg atgccgttgc
    22081 gatgaaagac tcgagtagtg ccgaagtttc tgtggctaaa gcgaatcttg agaaagctca
    22141 aatatcattg aacgatacgg aaattcatgc cccgactcac gggacgatac tgaccagaat
    22201 tcaggagcca ggggctattg ttaatgtcgc ggaaccagtt tatacccttg ctattgataa
    22261 tccggtatgg gtaaggactt atattgatga acctttttta ggtagaattt atcctggaca
    22321 ggacgctttg gtttacacgg attcgaatcc ggaaaagccc tacaaaggtc atattggctt
    22381 tatttctccg caggcagaat tcacaccaaa aattgttgaa accactcaat tgagaacgga
    22441 tcttgtctat cgtataagag tcgttattga tcaaccggat aatggattaa ggcaaggaat
    22501 gcctgttact gtgaaaatcc atttaaaagc gaattcatag taatgaatag ttcaaaacca
    22561 ctagtcagta ttaatggagt cagcaaatgt tttcctggaa acactgatcc ggcattagat
    22621 aatgtttctg caacaatttt tgaagggcaa atcacgggtt tggttggacc ggatggggct
    22681 gggaaatcaa ctcttatgcg tcttatatgc agcctgatgt tgcctacgca aggaacaatt
    22741 acagtagaca gtctcaatac ccggactgac tcggaaaaaa ttcatgcgat tactggctat
    22801 atgccacaaa aatttggttt gtatgaagat ttgaccatta tagaaaactt gaggctctat
    22861 gctgaactta gaaatctgca tggcgagaaa cgtgaagagc aatttagaac attacttaaa
    22921 tttacagcac tggagccttt tcagaaacgt ctggctggta atctctcagg gggaatgaag
    22981 caaaaattgg ggctggcttg tgccttaatg ggtgagccta agctgttgtt acttgatgag
    23041 ccgagtgttg gcgttgaccc aatatccaga agggagttgt ggactatggt ccaggggctg
    23101 cttggaaggg gtatgggcgt tgtttggtca actgcttatc ttgatgaagc tgaaaagtgt
    23161 gaccaaatcc ttttactcaa tgccggcaaa ccaatatacc atggaaaacc gggagatttt
    23221 ttgcaaaaaa ccaaagaccg gacttttcaa atttgcggtg tttccctgct aaatcgtcgc
    23281 caagctctga tgaatgccct taatacccct gaagtcattg atggtgtcat ccagggtaag
    23341 aatattcgta ttgtcattag tgataaaaaa ataaagccaa gtttggatgg tataactcgt
    23401 gaacccggag ttcattttca agaagttgaa tcgcgttttg aagacgcttt tattgacagt
    23461 ttgaaaacta aaataagtgg tcgctctttg ttagccgagc atattgctga aaaacctcat
    23521 agtgacaagc ctatcatcga ggcccgaaaa ttaacgaaac gttttggtag ttttactgcc
    23581 gtttcgaata atgaatttac aatcaaacgc ggtgaaatct ttggtttatt aggccctaat
    23641 ggagcaggta aatcaaccac atttaaaatg atgtgtggtt tattgcaacc cacacaaggg
    23701 caagcatttg taatggggct cgatctaaag acttcatcct ctgaagcaag aagccgtatt
    23761 gggtatatgg ctcagaaatt ttcactatac agtcatttgg atgccttgca gaacatgcgc
    23821 tttttttctg gtctctatgg gttatccgga gaaaggcagg aagcacagat caacagcatg
    23881 attgaaattt ttgatcttaa aaagcactta aagcaaaatt caggtgatct tccattagga
    23941 tttaaacaac gtctcgctct ggcttgtgcc gtaatgcatg agcctgatgt gttatttctt
    24001 gatgagccga cttcgggggt tgatccattg acccgacgtg agttttggac acatattaat
    24061 ggtatggtga ataaaggtgt gaccgtgatg gtaaccactc attttatgga tgaagcggaa
    24121 tattgcgatc gtatcgctct tatttacaga gggaaaaaca ttgccacagg aacgcctgat
    24181 gacttaaagg ataaagtacg taactccaca ttacccaatc ccactttaga agacgcgttt
    24241 atcgagttaa tccgattgga tgagttgcgt gagccggtac attaacatag ggagaatagt
    24301 ggttgaatat tcgctatcta aaggcattgg tcattaagga aactctgcaa attatccgtg
    24361 atcccagtac tatattaatt gcctttgtat tgcctttaat tttgcttttt attttcggtt
    24421 atggtgttaa tctggacact gatcaagtca agtttggcct ggctttagag agttatgatt
    24481 cgtcgactgt cagtctttct caatctttta gcagttcccg atttttggat gtatctattt
    24541 caacaaacag acaagcgttt cgttatgggt tggttaatgg aacaattcgt ggttttattg
    24601 atattcctca acgtttcact gctgatagtc tgagtggaaa tcccaagcca gctctgcaaa
    24661 acatagttga tggttcttat ccgcaagttg ccggttttgc agaaaattat gcgcttgggg
    24721 ctttgcaaac ctggattagc tcccaagctt atgagcatgg gcaaatgaca cttaagtccg
    24781 gcattaacct tgagcccaga tactggtaca accaggagct aaaaagcaga aattttctta
    24841 ttcctggctc aatagttatt gttatgactt tgattggaac attactcacc tcgctggtga
    24901 tcgctcgtga gtgggagaga ggtacgatgg aggcgatgat ggcaacgcca atttctattg
    24961 ttgacatcat cttgggaaaa ttaataccct attttgtgct agggatgggt tccatgtttt
    25021 tatgcactat tattgcaaca tggtattatg gagtaccttt ccaaggtact cttttggcac
    25081 tgacattagt ttcagccgtc tttctggttg ctgctttagg gcaggggtta ttaatatcta
    25141 ctcttgcaag agaccagttt gtggcttcgc aattggcttt aatgtctgcg tttttgcctt
    25201 catttattct ttccgggttt atttttgaaa tcaattcgat gccaaagtgg atacaattac
    25261 tcacgcatct gtttgctgca cgttattttg tgacaagtct gcaaaccttg tttttaacgg
    25321 gtaacatttg gtctcttttg attcgttgcc tttttgcgat tgcactgatt ggatttgcat
    25381 tcttttttat taccgcgcat aaaaccagaa aaaggcttga ttaatggcaa aattatctcc
    25441 agcaacaaga atttttgctc ttgtcattaa agaaatattg actttattca aagatcccaa
    25501 gggacgaatt gtattgatag ctcctccact cctacagtta ttgatttttt cctttgcagc
    25561 tactatggaa attaaaaata tttctatagg cattttgaac caggattccg gaaagcatgg
    25621 ttatgaagtg attcaacgct ttataggctc accaactttt tcaaaagttt tttttattca
    25681 aaagcaatct gatatcaaac ctatcattga taaccaggaa gcaatggttg taatgaccat
    25741 cccacaagat ttttctcgta aaatcgaatc gaattcacaa gccaatattc agttaatttt
    25801 ggatggtcga cgttctaatg caactcaaat tgtaaatggt tatatcagta gtattattga
    25861 aaactataat caggaaattc aaaaaacgca tggtgcaatc atgacacctc ctgtgatggt
    25921 cactcgaagt tggtttaacg aaaatttgct ttatctttgg tttaccgttc cttcattggt
    25981 ttgcatctta tccatgctga tttctcttgt ggttactgct ttatcggtag ccagagaaag
    26041 agagctgggc acctttgatc aattattagt atcacctctt actccctatg aaattcttat
    26101 aggcaagact attcctgcca ttatcattgg tttggcggaa ggtctcttga tgtgggttgc
    26161 tgcggtttgg ctatttgatg ttccttttta tggttctccg ttactgatgg tttatatttt
    26221 gtttattttt attatgtcaa cggtgggtgt tggcttattt atttcttcta tgtgtaaaac
    26281 ccagcagcaa gctattttgg gtgtatttat ctacatggtg cctgttgtga cgctttccgg
    26341 ttacgcttct ccggttgaaa acatgccgga atggcttcag gatttaacgt ggtttaaccc
    26401 attaaaattt actttaatag cagtaattgg cattttttta aaaggtatgt cggctttaga
    26461 ggtttttatc acaacatggc cacttctatt ggtaggggct gttacattat ttattgctgg
    26521 ctggtttttt aagcagcgta tggagtaaga aacagtatgg acagaaaatt agcgctactc
    26581 ttcagtattt cttgtttatt tggctgtact gtcggtcctg attatcaacg cccagacatt
    26641 aaaattcagg agaaatggcc aacaagcaaa gtagagaaaa aaatttccag tagtgcccct
    26701 gttcatttaa cctggtggaa ggcatttcat gatccattgc ttgatcgata cattaacgag
    26761 gcagctacta ataaccttga tatcaaagca gcagaagctc gtatccgcca agcccgcgca
    26821 atgaaacaaa tttcccttgc caatttttat ccgcagatca atggatcagc aagtgctaat
    26881 tccttgcaaa tcagtaaggg tacgttggag gggatttttg caaactctcc aacatatact
    26941 ctaattgata gaaatcaatg gatatataat ttgggttttg atgcacaatg ggagttggat
    27001 ttatttggta aaacaagaag aaaagttgaa gcaagcaaag caagcctgga agaggcttat
    27061 gaatcacgcc gtaccatttt actatctacg atagccgaga tcgcaagaag ttatatagaa
    27121 ttgagaggcg cgcaacaatt gtatggtatt gcgcaaaaaa atgtggaatt gcaaagaaaa
    27181 acgttggctc ttgtccgcca acgctataaa gcaggtgtta gcagcgaagt cgatgtttct
    27241 ttagccgaag gccagttaaa aaatacagaa tctgttttac ccaattatat tacccaaata
    27301 caggtaaatg cttatcaaat tgccgttatg ctgggtaaag atccgcaaac aattctcaaa
    27361 gatattgcaa aaattcaatc tttacccaaa ccaccgcaaa aagtacctat gggattacgt
    27421 tcagatattc tgcgccagcg tccagacata agagcaacag agaggcaatt agcgtctgcc
    27481 gtagctgaaa ttggtgtggc agttgctgac tttttcccgc aatttaatct ttctgtttcc
    27541 aatcttgctt ttcagagcac ttctgttgat acgttatttc aatccagtag tggattttgg
    27601 ctcatgggtc ctcaagtcag tttacccata tttcatggtg gccagattag agctaatgtt
    27661 gagagtaaga aggcggttgc tcagcaagca gcaatacaat acaaaaaaat aatcctccag
    27721 gcattgcaag aggctcaaag tgctcttgtc cgctatagcc aatcgctcga tacaaaccat
    27781 agcttgcgcg ctgccattat tgctaacaag cgagctgtgc aatttgccaa gaaacgattt
    27841 caatacggtg aggatgatta tttatcacta ctgcaatcag aggctgctct tctgaatgcc
    27901 gagactcaag aagtcaattc ggaggtttct tcattgactg ctttgattag tctatataaa
    27961 gcgcttggcg gaggttggga aaactttgag gcttaaagta ggcctaaaca tctgtgcatg
    28021 ctctaaaatc aggaacttta tcaataaatt aatgtaataa gttatggata aggtaggtca
    28081 ttatcttgaa tcgttcgggc tgaggaggca tttatgccgt ctcgaagcct tgtatggagt
    28141 ttaatgtttc gtgccaaggc ttctagacga tgcttactta tctcctaagc ccaaacagcc
    28201 cctagatatc gacagcttct tatataatcc ttatccatta cgttaaatta gcaaaaatca
    28261 gacaggtagc tcgcaaacgt agatctggct atagaacgat ttacgtttac taccatgaaa
    28321 atatctctta caaaggtgtt agaaacctat tcatatcagg ctgttggttt aaacggtact
    28381 gataaacaat ataaaaggcc attacatttt ttagataatt tcgtgtttct tgccagggaa
    28441 gtgtttcaat ccagatatct atttctttgg gcgggtgatt tttaagccaa tagactactt
    28501 gtctgggacc ggcgttgtaa gcggcagcaa caagaaccgg gtggttgctg aagcgcttgg
    28561 ttaagtgctg taaataagca atccctatat tgatattctt ttgagataaa aacaattgct
    28621 tatgatccga atacggtatt ttgctggttt tggaaacagc gctggctgtt tgtggcatga
    28681 cttgcattag ccctttcgcc ccagcagagg atgttacatc ttctctaaaa ccactttcct
    28741 gacgaataat agcatataca agctcaggcg gtatggcgta ttttgacgca tatactttta
    28801 ttgtgtcttt gtaggccaat ggaaatctta atgctaattg attattgagt gtgtcattat
    28861 tgcttaagta aactgatttt ccgtgccatt ttagttcggt tgctatccaa tagaccagag
    28921 cacttgcttc ttctttgggt aattcactaa tgaagtcatt taataacctt gatgcttgca
    28981 gtagctgttt ggagttgtaa aaatttttaa cttgttctat gacaggttga tagggtttga
    29041 gaatctctgt gttggttgtt ggtttttcat tgtcaaagct gggtcttttt ttcaggcgtt
    29101 tgctggctag aaaaccataa taatgtctgt ttttagctag tggctcataa attttaatcg
    29161 cctctgcttt tttcccttgt tcttccaggg agcgagccaa ccaatattgc caacaaggca
    29221 tctctttatt tggtgagttg ttaattaatt tttcaacttg tttccagttt ttatttttca
    29281 gagcaaagcg gatttgccag tcgactaaca catcggtgta gtattcaggt ttgattttgg
    29341 caaaccattc ctgagcatcg gcatgatttc tcatggcctt gtataatgcc aaatgtgcaa
    29401 gaaaatcttg tttttgccgc tcatttaaca tgatgttggt ttttttctct tgccatagtt
    29461 tcactgcttt atccatgtta atggatacta atctttttaa tccataaaga tagaaataat
    29521 cgttcaattt gccagggttc aacttggaaa tattggaagg attctcataa acactggtta
    29581 agttttttat ttctgtatcg tgaggtgttt tgtattgttt taacaggtaa cgagcgagta
    29641 gaacattacg tttgtctagt gcaagtgcta tgcgttgcgt aatgaatttc tgatcaaagt
    29701 tatcctgctt taacagtaat gcaaataggg tattgcatgc ctggggtctt gattctcctg
    29761 ataaccagat tggaatagag cctgctatag cctccttgta taatcctaaa ttgaatttcg
    29821 caatttgcgc aaagcatctg aggtttatgt cgttggtagg ttgataatac ttattataat
    29881 cttcccaatc ctttatcctg gccagttcat ataaccattt gtcacgcaat ttattagcaa
    29941 gcggtgtgtt ttcttttaca aattccaaaa attctggcga tgggttaata ggaagctgtt
    30001 cataccatgc aagatagcga ttaaacctgt ccatgtaggc ttgtccagat aaagcaaaca
    30061 agttgaaaga acagagaagc ccacataaaa aaattaataa ttttttcata attcctctta
    30121 ataaggcttg agggcaatta tttggcactt ttaaatgctt gctaattgtt cacgcatatg
    30181 tttaatggtt tcactataat tactgctatt gaatatagca gaacctgcaa caaattggtt
    30241 agctccggca tgtgctaatg aagcaatatt ctggggtgtg actccaccat caacacaaag
    30301 atcgagattt gggtgctgtt gccgaatttt agagagcttg ggtataacct ccgggataag
    30361 tttttgaccg ccaaatcctg ggttaactgt catgaccaaa acaaatttta gattatgggc
    30421 acaccaggtt aatgtgtcga ttgacgtagc tgggttgagc actaaacccg cttcacatcc
    30481 aaggttattg attagctgca aactcctgtc taaatgaata gtagcatctg gatgaatact
    30541 gatacgtttc gcacctgctt tggcaaatgc ttcgattaga gtatcaactg gagtggtcat
    30601 caaatggaca tcaatcggca aatttggaaa ttttttgatt aaagcgtcac agaaagcggg
    30661 gccaaaggtc aggttgggta cataatggtt atccatgaca tcaaaatgaa taaaatcagc
    30721 gccggctttc ataacggcat ctacttcctc tcctaaacgg gtcatgtccg ctgacagtaa
    30781 agagggtaaa atttggaatg tcatgattta gtaattcaac agtttgcttt tacatataat
    30841 aagctgactt tggtaaacat tccagcatta cgatttggat tcatatcgag ttttattgtt
    30901 ttaatcctgg atttgagttg gagcaaatca agataataat actccatcct ctctgattcg
    30961 ttagagagag gatggtatag aatgcttgat gtttcgaact atggagttta aagattcaat
    31021 taattgtcta atttcaaata aaatcgtctg attaaataat caattgataa tttccctggc
    31081 ccggtacata aaagcattaa aatcatgatt ccccattgca tgtgatcggt aagaccaacg
    31141 gcaccatccg gcgtccatag gaattgataa gagaaaacgg caaataagtt aaatagaaaa
    31201 agtccaaata aaacgagtct ggatccaagt cctaatgtaa tcaatacagg aaagcccagt
    31261 tcacaagctg ttcccagata cgcagctaaa gtaggcgaca tgaccggaac catgtattcg
    31321 ttttggaata aagaaagagt agcctgccaa ttactgattt tggttaagcc agatagaaaa
    31381 aaaacctggg caagataaag gcgggttaaa aggataaaaa ttggggcaag gaaatcaaaa
    31441 attttatcag ccagcagata ggtttttgct ataagtgctt tcatatagcc tccagtgaaa
    31501 aatgattaat ccattgataa cgaagcgcct tggttagagt gtcaccaatt ttttcttcgc
    31561 taatccattt acttaattct gttaccactt tttgccatgg tgtctggttt ccagaggata
    31621 agagttccag aaaaatatat tcctcctcac taattagcat aatacaaggt aaatcattat
    31681 gctgataaat aacaactttt tcaggacaac ctgatatttg tatttgttta tcctgctgaa
    31741 aatagttcat agcccaaatt tggtaaatag gatattcaga acagagaatt tttaaattgg
    31801 gtgtcagatg aaaacataaa gactgaaagc taatctcatc tatgctcgaa aaagcctcaa
    31861 aatcaaaagg gttaacaggg gaaagaagct ttaaggtgtg ccagtgccac tcaatagcgc
    31921 ataactgctg caagtaaggc agctctttag ccggctcaaa attcgcaata aattgaggaa
    31981 ggctatctcc gtactgatta atgtcatatt gagtggattc gcaattattg atatagcgat
    32041 gcaataaagc gtaccagaag ttttccccga ccaaacgcat acagagagga aaagttgatt
    32101 tcaaatgatt aaataaagta gcatggtggc tttccatgta gacactaata ccatcaaaat
    32161 tggatcgagc gagcctgttt tttaaggggc ttgatagtgc ctcctctttg aattgtgcta
    32221 tccattcctt ttgaattgtt tcaagcagag tattacgcat aatgattcat actcctttct
    32281 gccacacgct ttattatggc ttttgcttta cacatttcgt catacaggag tgaaaaatct
    32341 ggcaagtcat tatcccactc gataactgtt ggcgtgttta taaagagatt ttgagccttt
    32401 tcataaagtt gccaaacgtc ttggtatact ttatcactat gactatccag caaatatccc
    32461 tcttgttttg aaaatccccc aagatgcatc tgccatacgt ttttggtatt gatcgaatga
    32521 agatacttat ccgggtcatc accatgatta taccaattca cgtataaatt attgatatcc
    32581 aataagatac cacaacctgt agcagtactt aactcattga gaaactcggc ttcagataat
    32641 gtattttgtc gataagtgac gtatcttgat acgttctcgt acattattgg ctggtttagg
    32701 tattcctgaa tgcagtttgt tttttcaata atgaatgaga gggtttcttc agtataggga
    32761 aacggtatca attcatgggt aaaaaattga ttggcatggg tccagcataa atgatctgat
    32821 attagcttgg agtttaaaca atgagccaaa ttttttattt gctgcaaata ttgataatca
    32881 ggttcagagg atgtggcgat agataagcca acgctgtgta aactcattgg ataaaactct
    32941 gcaatcttga gcaactgttt aaattgagct cccgattgtt tcatgtagtt atcagccagg
    33001 acttcaaaat aatcgactgt gggctgttcc tttaaaatat cgtcgatgtg ttcaagccgc
    33061 aatccaatgc ctgaacatcc agaataaggc gcggcttgat cggtagaatg atttgaatac
    33121 attctaatta gcttttaact gtgccgttaa ccagcttttc acaagtacct ttgggcagat
    33181 aaatccagtc agttgttgaa ccatctgttt tagattgacc agcacaggca tgttgagctg
    33241 taccacaatc attttttcca gctttggcca caccttggca tttttccata tcatttggag
    33301 tatctgcatt agcaggtatg gtagccattg aaagtccagc agccatcacg gcagccattg
    33361 ttattttaga aatattattc atgataccta tcctttgtta tatgtaatta atccatatgt
    33421 tatttaataa accccttgcc taaccgggct ttttcattgt attcctgcat gcaaacacag
    33481 ctcctcaaaa tgtttatttt tttgctctaa aaaacaatca ggttaggcaa gaggtctaat
    33541 aaaataacgt agttagtata gtaccatttt tcgatttgtg acaggacatc aacttatttc
    33601 tagaggatgg aaaaatattt aacatggttt gtttttattc caaataggat agaaaacatt
    33661 tcttagatct ttgccttata cttaataaat cacagcaaaa ggaagaggag tattgtatca
    33721 tggcaaagga taccaaaatc attaatcttg ctttacaagg aggaggtgct catggtgctc
    33781 ttgcctgggg tattattgac agacttcttg aagatggtcg tatccatttt gatgccattt
    33841 ctgcaacgag tgctggtgct atgaatgctg ccgtacttgc ctatggattt gcaacgggtg
    33901 gaaaggaggg ggctcgagaa gctctgcatc aattttggaa aatggtgagc gatgccgggc
    33961 agatatacaa cccacttaaa aaaactccgt tggaagaatt attgggcata gggattgaaa
    34021 attcaatgtc cttttttatg tttgatttga tgaccaaaat tctttctccc tatcaattta
    34081 atcccttaaa ttttaaccca cttaaggaaa tattagagca aattgtagat tttgaaaaaa
    34141 ttaaatcctg taaaagcatt aaaatattca tctcagctac taatgttcgc acaggtaaaa
    34201 ttaatgtatt tgataataaa aaaatttctg ccgatgcggt tatggcctcc gcttgtctgc
    34261 ctttcatgtt tcaggcagta aatattggcg aggattattt ttgggatggt ggttatatgg
    34321 ggaatccagc tattttccct ctaatttata attccgagtg cagggatatt ttaattcttc
    34381 atatcaatcc tatttatcga gagcatgtcc cggatactgc agccgaaatt ctaaatcgag
    34441 tgaatgagat cagttttaat tcttcattaa tgcgtgaaat gcgggcgatc gcttttgtta
    34501 ctaaattgat agacagcggt tggattaagg atgaatacaa aaacaacctc aggaaacttt
    34561 atttacatgc cattcgcgca gatgtatcaa tggaatcctc ctcagtagcc agtaagctta
    34621 accctgagtg gtcgtttatt agccatcttt atgaagaagg acgcaaaaag ggagaagaat
    34681 ggctgaaaag taattttaaa catcttggaa aaatgaccag tattgatctg gatgaatatt
    34741 tataagttta ttatcagaat gatagttcac aacataatta gaaatttgca gctataatta
    34801 aaaccaaagt tattagaaat acacagatat tttaaaaact ttcttgaaag ggattttgat
    34861 atgcagcaag aagatcgggc accgataact caaaaaaatc gttcgttttc aagaaataca
    34921 gcgaggtgct ttgacacctt tatggacact gttgactttc ttggccggat tggatggcct
    34981 gttattgatt tggcatttcg aatatggata gcccagcaat tattagtttc cgcaatactt
    35041 ctaacaaacc actgggatac tgctgtttat ttagctaaaa atgaataccc ggttccatgg
    35101 ctatcacctg aatgggaagc tttgttaggc atattggcgc agtttgtagg ggggatttcc
    35161 ctgcttttgg gtttgtttac ccgatgcgga gcggcagtta ttattggatt cgctgtcatg
    35221 actcaagtct attaccagcc tatcgatctt aatttgcttt ggattgcatt aatgttgggt
    35281 tatgtgttgc gaggcccggg tccactttct cttgataatt tgtttgagca tgggtttagt
    35341 cgcagccctt tgccgtttgc catgttgatt gtgacatttg tagataggac aagagaattt
    35401 ttttcaagtt tgtatttatt ttctttacgt atgtggttaa tgatttcact tttactcatc
    35461 aatcaacaca tacagctgat ttcttctgat gcgctgccgc tattgatgcc atggttgcca
    35521 ttaaattcag cctctatcgt ttttaacaat gtttcactat tcatggctat ttttttggga
    35581 cttggcttag cgactcgagt tgttgcaatt tttggattaa tagttgttct tttgagtttt
    35641 acgcccggag taaccggttt ttatttatac tggatggtgg tgatggctat tttatttatt
    35701 tcgggtccag ggatcttgtc attggatcat ttgatctttc atgctttgaa gaaacactat
    35761 cctcagcttt ctggaaaacc ggcatttagc ctggatggtt tgccacgagt agttattata
    35821 ggcgcaggtt ttggggggat tgcttgtgct aaggcactac gtcatatgcc agtcaagatt
    35881 actttgattg atagacacaa ttatcatctt tttcaacctt ttttatacca gattgctact
    35941 ggaagtttat caccagccga tattgctatt tcgattcgct cta
//
LOCUS       DASLOE010000016        35935 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.016, whole genome shotgun sequence.
ACCESSION   DASLOE010000016 DASLOE010000000
VERSION     DASLOE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 35935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35935
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.016"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            181..2205
                     /locus_tag="U6309_000612"
     CDS             181..2205
                     /locus_tag="U6309_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEN5504067.1"
                     /translation="MELRIDNTPFKAIFQPLDLGFTQIKNRLLMGSMHTGLEEDKENL
                     NRLASFYRERAQGGVGLIVTGGFAPNRAGRLAPFAAKLTNSKEQHRHEVITQAVHEAG
                     GKIALQILHAGRYGYHPFIVAPSSIKSPISPFKPWEMSKRRIIKTIKHFARCARLAQL
                     AGYDGVEIMGSEGYLINQFIVTHTNHRRDEWGGSFSNRIRFPIEIVRSVREAVGEQFI
                     IIYRLSMLDLVAEGSNWQEVVTLAKAIEQAGASLINTGIGWHEARIPTIGTMVPSAAF
                     TQVTQHLKPEIKIPVITSNRINTPELANQLLEDGVADMISMARPFLADPCFAEKAKLG
                     ESKAINVCIACNQACLDQVFVNKTASCLVNPRACNETTLVYETTNQPKAIAVVGSGPA
                     GLAFAVTAAERGHKITLFEKANQIGGQFNLAKKIPGKEEFQHTLNYFAYQLQKYKVDV
                     RLNTEATVELLKDYDEVVLASGIIPRVPEIKGIDHKKVVSYIDVITQNKPVGSRVAVI
                     GAGGIGFDVAEFLTHDHQLTSEQFYNEWGIDISGQHRGGLKPPEIAPSPREVYLLQRK
                     KEKMGKRLGKTTGWIHRSSLKHKQVKMFSGVNYERIDDQGLHLTIDDKPQVLEVDSIV
                     ICAGQIELKNLYEPLKMAGKLVHLIGGAYKALELDARHAIDQACRLAALV"
     gene            2202..3119
                     /locus_tag="U6309_000613"
     CDS             2202..3119
                     /locus_tag="U6309_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN5504068.1"
                     /translation="MSKIAVVINPIAGGGRGKKIWKVLKPGLHALFEQTVYRISNRVN
                     DLEAITDGLLAENPDSLLIIGGDGTLNHVLNGLIENDKLKNPQTKIAFFNAGCGGDFT
                     RQFPQQKMTEFLDRLSHNQFIKCDIGKITLANQVSRYFINIASCGLSGHVVLRVSKSK
                     WLKKLGGTLNYLLHALTGLMTYRKTKVRVQIDDALPFDSSILLMAVCSGQYFGGGMHV
                     APMAKVNDGLFDVVSFNEFSKLNALLKLYKIYSGGHLLDNNVHYVQAKKIKIEPLEES
                     KIEIEADGEIIGCLPAQFELIKETLPLIV"
     gene            3487..5256
                     /locus_tag="U6309_000614"
     CDS             3487..5256
                     /locus_tag="U6309_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN5504069.1"
                     /translation="MNQEISNFSTFNFSSALNKALEDMKFITPSPIQAQTIPLILEGR
                     DAIALAQTGTGKTAAFALPILQNLSPEISTTQALILAPTRELAIQVAEQFELLSKYQR
                     NVTIAVLCGGQEYGRQLKQLRSGAQVVVGTPGRILDHIDKGTLLLNNLKTFILDEADE
                     MLRMGFIEDVETILEKLPEKKQMALFSATMPYRIRQIANTYLNDPASIEIRMETATVK
                     SIEQRFLFASVHQKPDALIRVLEVEDYQGVIVFVRTKSSTEEVAELLQQYGLRAMAIH
                     GDITQSLRERIIAQFKQGAIDILVATDVAARGLDVERVTHVINYDMPHDNETYVHRIG
                     RTGRAGRSGVTILFVTPKESRLISSIERHTRQRIEKVQVPNDHMIQMARQQRFMSNIT
                     TRLNHEHVYSYKRIVEEYIKEHDVSAVDVAAALALLLHKDMPWRKELNMPKAASAFKE
                     ERARAGEKGLDLRGRFKDDRKSSFADRKGARGSKKDFAKDFSNERKKSNDGRKAVNQE
                     SVAQDLFRIDVGKIHGVKPGNIVGAIANEAGLQSRYITGLKIHEDHSTVRLPKGMPKE
                     VMKDLTKAWVCGRQLNLTLIGSA"
     gene            5607..5825
                     /locus_tag="U6309_000615"
     CDS             5607..5825
                     /locus_tag="U6309_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504070.1"
                     /translation="MKKKHEPGFFGRETKNIKDIESNRYDPDCPIRRADKSRGFSQKA
                     SGQFQLRIKEEVKEALDDIRNESAYKFN"
     gene            complement(5982..6608)
                     /gene="mavE"
                     /locus_tag="U6309_000616"
     CDS             complement(5982..6608)
                     /gene="mavE"
                     /locus_tag="U6309_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavE"
                     /protein_id="HEN5504071.1"
                     /translation="MTRFIMLSFVTGYRKSISCNRFSRNVVSPYFFMRTIIMTRFERN
                     FLINSLMFLETILSVDKKLDDAIHHFTQGQYENPRYQINSRITNADDWSKEDKLKFTS
                     AIAEAIALVSEKYENPTSETTEQIQSARNILLDDYVPILTANTDPENRLKSVRENSSQ
                     IRKELIAKLKDEVPYKSQFENPYVLFPFVAATVAVAATAASVLFGNKP"
     gene            6767..7696
                     /locus_tag="U6309_000617"
     CDS             6767..7696
                     /locus_tag="U6309_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN5504072.1"
                     /translation="MKLLASLCAFLLSGVVSATPLNNIVVFGDSLSDNGNLYEYMKHQ
                     LPQSPPYFEGRFSNGPVWIERLAASYFPNDPNTHLLDYAFGGAGVSVDEEDDEVFFTL
                     RREVNSYLLAHQDKASPDSLFVIWIGANNYLGMPVEVEETLKNVNRGITDSIQRLVDK
                     GAKHILVLNLPDLGRTPAALEFGSVEEMTYFSTQHNNALSNTVDYFKKTYPEVEWLFF
                     DTGSHFDYVIEHASEYGFTNITGTCSFSIVDEITKNSVLKMVASVKPELTESACDGYL
                     FFDLVHPTALAHKIMAEKARLMLDEAGVEFAEN"
     gene            7888..8661
                     /locus_tag="U6309_000618"
     CDS             7888..8661
                     /locus_tag="U6309_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3530 family protein"
                     /protein_id="HEN5504073.1"
                     /translation="MNQIKWIMLLFCFVVSNVFADKLDITVDQKKIILPYWLSRSVSY
                     GAVIIVSGGETAQWSLLLEQFAKGLSRNGWSVALLNCTKNNSIPWINQLPEVISTLRQ
                     NNNKRIVLVHYGDQLNLALEYFSKPQSKMINGLVMLSAYDLNRNLQKVPRLRFPLFDI
                     VGQFDYDMVRQQRKSREEKFKENSYLAIDIPGATHDYQYSQQLLLAFVHGWMAKLPEF
                     EPQPPPILVSYLEPVYSTASSVVPTNKLDYQPLVSQINS"
     gene            complement(8628..9518)
                     /gene="djlA"
                     /locus_tag="U6309_000619"
     CDS             complement(8628..9518)
                     /gene="djlA"
                     /locus_tag="U6309_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HEN5504074.1"
                     /translation="MNLRDFFVITTWWGKILGAFFGYLTAGPVGALFGILVGNFFDRG
                     LVSYYSNPHWLYHAEKQRIVQKAFFEATFSIMGHVAKSDGRVSEQEISMAKSIMNEMK
                     LSKGQKDLAKRLFNEGKQPDFNVSLALIQLQRICKDNRDLLKLFVDIQYRAAQVDGLS
                     SQKIHALDNIFAHLGFAPLHKQYRFYEDFGSYFQQEQSKQHYHNQQGYKHTSSSQDQQ
                     GYKPQSPPNTLAHAYALLEVSPNASKQEVRRAYRRLLSRNHPDKLIAQGLPEEMIKLA
                     NDKTHQIMKAYELICETKGW"
     gene            9673..10932
                     /gene="waaA"
                     /locus_tag="U6309_000620"
     CDS             9673..10932
                     /gene="waaA"
                     /locus_tag="U6309_000620"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEN5504075.1"
                     /translation="MRFVYSFLMYLLTPYLLFRLWRKGRKLPAYRQRIGERFCLGIQE
                     NAPVDVWLHAVSLGEVIAATPLIDAMLNKRWSVLVTTMTPTGSERVKSRFGHKVAHQY
                     LPYDLPWVLKRFFKRTRPRVGIIMETELWPNLINQAQASGVALFLANGRLSDRSLQGY
                     LKLKFLFKPVLNQFSGILTQSNEDAERFIALGANADLVHVLGNMKFDLQINSVDRSQY
                     RELKSHWGEDRPTIIAASTHDDEESQILSQLPRLQEAIPGVVLLIAPRHPERFQTVYQ
                     LSVQAGFNTGCRSNLNTISRENEVVILDSLGELLGFYQISDFAFVGGSLVPVGGHNVL
                     EPIAMNVPVLSGNQVHNFKSICRELKEAQAILLVNHANELVDAIIKLYQDRESQNTMV
                     ANASSVLESNKGSVVRYLQKIESALGY"
     gene            complement(10937..11770)
                     /locus_tag="U6309_000621"
     CDS             complement(10937..11770)
                     /locus_tag="U6309_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN5504076.1"
                     /translation="MFFMYFTNYGYRLNQIKKGSDQHWLFLPGGPGLGSEYLASFCDK
                     LTLQGTVSLVDFPKDGNNGEGQLDFVSWQKGLISLLKSLNKPILVTHSFSGMFTLNFP
                     EIENHLSGLVLMNTTTTNSFFQHVNEMRQKHNLPDLVPAASQYHLYPSNETYKKFWDT
                     YKHYCFTQEELPIGEQMMELFAYNNESYHYAIEHFYNDFHYKWHPQSIPTMTIASELD
                     YICPPAIFIQDKNFQCQNVINKVIYKAGHCPWILHLEQVQKCFDEFFGMIQEINIERT
                     L"
     gene            complement(11840..12316)
                     /gene="dksA"
                     /locus_tag="U6309_000622"
     CDS             complement(11840..12316)
                     /gene="dksA"
                     /locus_tag="U6309_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEN5504077.1"
                     /translation="MTGQLIDKTKERLYNVKNDAIGNMGIAPYQETEGEEYMNEKQLA
                     HIEKILLAWRQSLMEEVDRTVSHMKDEAANFPDPSDRASQEEEFSIELRTRDRERKLI
                     KKIEDALERLRNEDFGYCEACGIEIGLKRLEARPTATLCIDCKTLSEIKERQNQGA"
     gene            complement(12415..13278)
                     /gene="prmC"
                     /locus_tag="U6309_000623"
     CDS             complement(12415..13278)
                     /gene="prmC"
                     /locus_tag="U6309_000623"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEN5504078.1"
                     /translation="MIDIRSLLEQALQQLKVKNQETKLEAELLLCHVLNKNRAYLFAH
                     PDALVSPEQIETYLQMIKQRAEGLPIAYITGQREFWSLSLKVTPNVLIPRHETERLVE
                     LALELIPDKENISVLDLGTGSGAIALALAKERPLWHIDACDFSKEALELARYNAKTLG
                     LNNINFYHSYWFNNLPLKQYHAIVSNPPYIAENDPHLKQGDVRFEPTSALVSSQDGLA
                     DLQYIIQHSYEYLLPDGLLLVEHGFEQKNEISAILNQLGYKNIHCWQDLQGHDRVSGG
                     QRPSNRNNKPL"
     gene            complement(13271..14359)
                     /gene="prfA"
                     /locus_tag="U6309_000624"
     CDS             complement(13271..14359)
                     /gene="prfA"
                     /locus_tag="U6309_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEN5504079.1"
                     /translation="MKKSLELKLQQMLERYEEVGRLLSEASIIADQNQFKSLSKEYAQ
                     LEPVSQCYESYLEAKNNLDSLNELLESDDKDLATMAEEEIDTVKKQIEELDEQLQWHL
                     IPKDPDDERNIYLEVRAGTGGDEAAIFAGDLFRMYSRYAESQGWQIELISASHGEHGG
                     YKEIIAKISGQAVYSQLKFESGAHRVQRVPETESQGRVHTSACTVAIMPEVDEINDIQ
                     INPDDLRIDTYRSSGAGGQHVNKTDSAIRITHIPTGVVVECQDERSQHKNRAKAMSLL
                     KTRLLDAEVSKQKQEQAQTRKSLVGTGDRSERIRTYNFPQGRLTDHRINLTIYQLSDI
                     MEGNLSLVIDPLKREYHAELLADLGRHD"
     gene            complement(14343..15617)
                     /locus_tag="U6309_000625"
     CDS             complement(14343..15617)
                     /locus_tag="U6309_000625"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEN5504080.1"
                     /translation="MVFVACGLNHKTAPIHVREKVALQPAMQDSLLSSLLDLPEVNEA
                     AILSTCNRTEIYCDTNTPEVLGNWLAHEHQLSEELLSQFLYIHQGKEGIKHTLRVASG
                     LDSMMIGEPQILGQMKQAYQHACRLGTVKTQLRPVFEYIFRASKRIRTRSGIGANPVS
                     IAYAAVQLIGQLFKNYHSLSVFLIGSGETASLVAKYLHQHGVHRFLIASRTLENAQKL
                     AETFGGKTLSIGDIPQYLPLADVVISATACPLPFINKSLVEHALEQRNHAPMFLLDLA
                     VPRDIEGNVNELEQVHLYNVDDLQSMIEKGMDERRNAALQAEQLIESELDNYIRWHRS
                     LRAKDVICDYRNQMHTLAQQELQRALKKISAGQNQQDVLNEFSMRLVNKLTHNPTIGL
                     RQMAWDNREDLLDLARYLFDTTANQSLYEEIS"
     gene            15936..16214
                     /locus_tag="U6309_000626"
     CDS             15936..16214
                     /locus_tag="U6309_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504081.1"
                     /translation="MDIILQGQHNAIETVESLERVLKLFKERYQVDGFREIHLTVTLV
                     DEQGDEVELVDSETNQPYRIFEVYRKGYELAGRKGIPMLQLVVDNTHK"
     gene            16271..17344
                     /locus_tag="U6309_000627"
     CDS             16271..17344
                     /locus_tag="U6309_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEN5504082.1"
                     /translation="MIMALVAIILTLILVIGVHELGHALTARLFQVKISKISIGFGKP
                     LIKWQTQSGCNWIWAMWPLGGYVQLLNSRISPVKPQENAYCFDKKPIWIRVLILLSGA
                     IANLITAWIALVLVFYIGISYKLPQIQSVKLDSLAAKAGIQAGDQWVAVENYPTDSWQ
                     GVGMQLVIHWGQKDVHIVLRQANQQLKQLNLDLSQIEFTSKDGSLLESLGIKPDLSAV
                     SSLTRYPSLLASMQKAFAEIIHTMYFFIMILKQLFLGVIPFSILLGPLAIFSVSVASL
                     TQGVIVFLLFIATLSLAVALVNLFPIPGLDGGSILYSVIEKIRGKPVSVAVEVLLHRL
                     MIILFCVLLVHLLMNDLNRYLHH"
     gene            complement(17373..17963)
                     /locus_tag="U6309_000628"
     CDS             complement(17373..17963)
                     /locus_tag="U6309_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEN5504083.1"
                     /translation="MKQLGSCCQYCAYPLSDDTYLVCGQCVRKRPSFDSAYIAYRFEE
                     PLRSLIHQFKYHNGLYLASFLKQLLLNALPKSALKPDCLIPVPMYPKRLKRRGFNQAA
                     VLTRLLARQLKIPYDLHYCQKIINTDSQANLDGEQRRKNLRHAFYVSPVTYEHVMIVD
                     DLLTTGSTANEMAHTLKNAGVKRVDICCCARAVTKN"
     gene            18121..18975
                     /gene="bioC"
                     /locus_tag="U6309_000629"
     CDS             18121..18975
                     /gene="bioC"
                     /locus_tag="U6309_000629"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEN5504084.1"
                     /translation="MTVNNEISKAFSKHADDYERVAKVQKEIGSRLFERLQYLKIAPR
                     RILDLGCGPGFFSKELALLYPKAQIVGMDLSFAMLEQARKKQGWRRKWPLVSADMQKM
                     PFATGAFDLVFANQVIHWSSSLGTVFRELNRVMNVNGCLMFTTLGPDTFKELQTAWSA
                     ANQYAHVNEFVDMHDIGDCLIAEHFMDPVIDMELLSIHYETLPQLLLALKTQGVRNIN
                     PKRNHGLTGKSAWKQFEAQYATMRTTTGKYPLTYEVVYGQAWKGAQRKMDQGIETWIP
                     VSRVVKKS"
     gene            complement(19019..19915)
                     /locus_tag="U6309_000630"
     CDS             complement(19019..19915)
                     /locus_tag="U6309_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN5504085.1"
                     /translation="MNKQVIDKYTQRHELYLEQLLNETIIPAPKIRSALHYALFSGGK
                     RIRPILVYLAGDLIDVDQGVLDIIAAALELTHCYSLIHDDLPAMDNDDLRRGKPSCHK
                     AFDEATAILVGDGMQALAIEVVLTRLSPLLPAAQVVAITQVLVNASGISGMVSGQSLD
                     LSELAKSSVTEEQLREIHLLKTGKLILACFEMVLAAQHEVSEQIKSALRTYGKHIGLV
                     FQMQDDYLDLYAPTQILGKGRSSDQANQKTTFATLFNKQQLEEEIAVHYQIAMDSLRL
                     FGSKAAALIELTKQLQNRSNLS"
     gene            complement(19896..20126)
                     /locus_tag="U6309_000631"
     CDS             complement(19896..20126)
                     /locus_tag="U6309_000631"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEN5504086.1"
                     /translation="MSKGIHFEQSITELEEIVRQLEKGELTLEESLKQFEKGISLARR
                     CQNALNQAEQKIETLTGTDSNIELDSDEQTSD"
     gene            complement(20320..20703)
                     /locus_tag="U6309_000632"
     CDS             complement(20320..20703)
                     /locus_tag="U6309_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504087.1"
                     /translation="MPAFRKGQNPVTELTRLKEYMEDQIAKAKESSSLTAQLKFLENA
                     HTEHFVKMGSLTTIYKGGSEVVDRLKIEIRSLYDEMLELKDKCRDQIQQQDASMKHSP
                     AFFTTRNSKTKTEEFDLDFDKAFGL"
     gene            complement(20967..21845)
                     /locus_tag="U6309_000633"
     CDS             complement(20967..21845)
                     /locus_tag="U6309_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5504088.1"
                     /translation="MAKFTVGQDPVKGLTELKAYMEEQISKIKKATTEQDIDQLLIDT
                     LNEYDDKMGSLSKVYKGGNEQVEQLKIDVRKLYEPLRDQFSHKHPLTLVSEVQTKLEE
                     QRKQAEVEKLKREEPLKLEKKIEEEKLKLAKQSEEEKPKLENQQEETITQALQKVDGI
                     IQELTLKIDRVDQHQYKKAHDTANTLLQSLIAARDEYERDLRANEFSQELAGRKFKLA
                     CQDAVKIAKPVLEKDLGWGDYLKNLLKCLGNAVITCFTFGYQQGFFAYARPDSAKAVE
                     KAEEDLGLRQAASSPQ"
     gene            22080..23432
                     /gene="radA"
                     /locus_tag="U6309_000634"
     CDS             22080..23432
                     /gene="radA"
                     /locus_tag="U6309_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEN5504089.1"
                     /translation="MKVKTQFVCNQCAAIFKQWAGQCTQCGAWNSIVEEGIVPENKTS
                     RSGQWANQRSVITAVEDVVMDKIVRMDCGISELNRVLGGGLVYGSVVLIGGDPGIGKS
                     TLLLQTLANLSMQETVLYVTGEESLQQVAMRAQRLGLPLAGLRLLAETQVESIIAQAQ
                     KEKPRIIVVDSVQTIFTEAISSAPGGVSQVRESAAQLVRYAKMTNTAIFLVGHVTKEG
                     TLAGPRVLEHMVDSVLYFEGQNDSRFRVIRAIKNRFGAVNELGVFAMTDKGLKEVANP
                     SAIFLSRQPEPTPGSAVMVTWEGSRPMLVEVQALVDEAHGQQAKRVTAGLEHNRLAIL
                     LAVLHRHGGIATYDQDVFINVVGGVKVTETASDLALLAAVISSLRNRIFDSETIIFGE
                     VGLAGEIRPVQSGQERIKEAIKHGFKRAIVPFANAPKQQLDSMTVEPVKHLRDVLEKM
                     "
     gene            23429..24265
                     /locus_tag="U6309_000635"
     CDS             23429..24265
                     /locus_tag="U6309_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEN5504090.1"
                     /translation="MSYQKPITPEYKMNEDTIRDFNRDGAVCLRQFLNAEEIAILKEG
                     IEFNLSSLSPRAKIASRHDDPGYFVEDFCTWQNNPFYQRIIFGTNLGVAAAQIMQSQS
                     VRLYHDHLLVKEPGTRQVTPWHQDQPYYNIEGKQNCSFWIPVDPVSRSSTLEFIAGSH
                     LGPWLMPRTFLDNQAKWFPEGSLQELPPIEEARARYPIIGWEIEPGDVVCFHMLTLHM
                     ARGVEGRHRRRVFSVRFLGDDITHAPRAWPTSPDFPGLTSEIPAGASMDHPLFPCLPL
                     KI"
     gene            24553..26481
                     /locus_tag="U6309_000636"
     CDS             24553..26481
                     /locus_tag="U6309_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-gulono-gamma-lactone oxidase"
                     /protein_id="HEN5504091.1"
                     /translation="MALPTELKSKFIQILNQNKISHSVGWSNYMESVNNPAAIVVEVK
                     NEKEVQTVMKAIKELNSDREPKDKVTVRATAGWTDKPGKSCCFFPWSEAQESQYNEGF
                     SFSQVVGGKESPDTAGTDVIIRFAKKFHHAKVIGSLKNPPVFNPDNPIHRLPVTLVEV
                     SAGMQVADFADFLREHQLSLSTVSMISWVTPVGLAGTAGHGTGRDEPAFSGLIESVTV
                     CDMDGNLREINSDHPDFKDLTGGHSGLLGIVVSMKLRAVKAFNLCETIELFPNTREMT
                     GRLSDILKNNQYISIMGMPSYGCPESDKLVSKWQIRQWNYTTDKPGKKEKAPYDPDVR
                     SFAQELQVRIGASVMDFLLDSGLKHLLPSFMLLSAAVVTGTRGTSPKVDYENHITHPQ
                     VAFPKEMRDVSYLIPVKDDEAGQKLELILQKMEAMLNEAGSKGEFPVTYAIYVRYLKG
                     TNGGLSTSFTSSDDERILAIDVVTHPEAPGITNFEKGFMSFLKEMNIIPRNHLGKNFP
                     AGVVHYDQFLGAERVSKYKQALERFYCSPGKNDGAERLSMSPFYTPYLQGMLSASAPT
                     KQLVGENKYEQRIERLHEEHSDLECAEFLSKLYAEVSRMSTSSEEALTAKKGFLEACK
                     AELEHRNSRLEAPVPLLS"
     gene            26571..27692
                     /locus_tag="U6309_000637"
     CDS             26571..27692
                     /locus_tag="U6309_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEN5504092.1"
                     /translation="MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGLVPIKS
                     NQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGRFRINLFRQRGEVAMVVRYIKGE
                     IPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIED
                     PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYA
                     ETGHLCISTLHANNANQALDRVINFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQR
                     VAAVEILINSPYISDLIEKGKIDDIKEVMARSTEQGMQTFDQALFNLYKEGKITKENA
                     IKYADSKNNVGLQIRLGEEKNFNIQDENLQIQPDDIDKF"
     gene            27779..29704
                     /gene="ankK"
                     /locus_tag="U6309_000638"
     CDS             27779..29704
                     /gene="ankK"
                     /locus_tag="U6309_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkK/LegA5"
                     /protein_id="HEN5504093.1"
                     /translation="MPRVYNLKDIYLGAPSFSGHEVYLDAVYYPSDPSEKNFRVIYKK
                     NKFGNANLSRMEVAFSQLARLFLDNGLTSFQKMVVNDANKVQGLIVEHLNYVIENKEG
                     LKQPFYTLNAPRNGCDYTEKRVTSSNEIPFYFLDKLPQGFFNQLLAAEKNNKLNIDYA
                     SLASILATSYTLEEDDLHKGNFGFYLVKKKGKPRVVFFKIDHDLMFVDSIMSFTTRRF
                     CHLFDGCDAFDITEEDLLKFPNLKYSANGYWPTKTSFFYKPWDNKDYRTYAEIQAFGD
                     LSHVEEFNKAKWRSFYKHILISKSQMEATLKACFDENNSSDRAHISLVIQAMLARQAR
                     LKAMLFSLKDFRDFILSQNGKERDLLCHEILNNLPEEERKSFENEIRQSLDYNHNLCC
                     SGLFEDGDTPLHIAIKSGDYRYDETIGMYGQFINTKNSSGKTPLDIALQMAGQSKVYP
                     ADVRKDYRFIMKHLLANGANQTKQFEEFDKIENISSYQFHTPYLNKAIKAKTYHELKE
                     VLRDIGEDHQYCLKFKKMLAVECISEFIKANQDNLSLRGILLKLKKEVDGKGTKSENA
                     ALMYIRQLRSRLWIVRQIRGLYGWSTTQGEIDYMIDKELARLDTKDLKRLSLFDSRDS
                     STLDNVFLDISLSKNKI"
     gene            complement(29798..31093)
                     /locus_tag="U6309_000639"
     CDS             complement(29798..31093)
                     /locus_tag="U6309_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HEN5504094.1"
                     /translation="MSSNESAAIVSPIVNQKLSQWKKQDTIWMLSLYGTAIGAGTLFL
                     PIDAGLNGILPLIIMTILAFPMTYYSHRALCRFVLSGSSSHNDITDVVEEHFGSFAGK
                     MLTILYFFAIYPILLMYSVAITNTTESFLINQMGITPPPRALLAIILILGLMGIVRLG
                     QEIIVKSMSVLVYPFATILFLLSLYLIPNWNNAIFIQSNSLHANGGHGLLMTLWLIIP
                     VMVFSFNHSPIISSFAVNQKQQYGSQADEKSSLIMKYSHLMMVFSVMFFVFSCVLSLS
                     PQDLLQAKQQNISILSYLANHFNTPIMAYIAPVIAFIAISKSFLGHYLGAKEGLSGII
                     VKSLKSTTGKTINKNSLHRIIEIFMILTCWIVATINPNILKMIETLGGPIIAVLLFIM
                     PMYAIAKVPAMKKYSNQISNVFVTVMGIISISAIIYGLI"
     gene            31338..31811
                     /locus_tag="U6309_000640"
     CDS             31338..31811
                     /locus_tag="U6309_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504095.1"
                     /translation="MFLEPGLINGVVMKRIILFMIAWSLLNSAYSSHSLPSHASILFT
                     LANTERSNSCPALLHNAKVVVDYDYNFERNMGLSHLRQLQSTKWSETLHPLGLSNYYA
                     FMSDMKPKAVQIEGGEVTIYRIIFHLYKNGDSRIYLMINQDGECVMSSDVVNVNL"
     gene            complement(31877..32815)
                     /gene="motB"
                     /locus_tag="U6309_000641"
     CDS             complement(31877..32815)
                     /gene="motB"
                     /locus_tag="U6309_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEN5504096.1"
                     /translation="MSEINDISDPINDDLEKKDKREKKEKTTHYTPIIRKIKKNDHKH
                     HGGSWKIAFADFVTAMMAFFLLMWLVASLNKAQKAGIAEYFKQPMKVALFGGESMGNR
                     KATIKGGGPNIEDTDGQVSATNKPLINESIAKDKEIVESQANEIKKLEELKSEINLSM
                     EKDPSLAGLKKQLLMDVVSDGLRIQLIDNQKKPMFDVGSDKINPEIEPILANIAKLLN
                     SVPNKITIQGHTDANPYHNPEELEYTNWELSAQRANAARRALIKAGMDENKVMTVTGY
                     SSTVLLDKKNPYNPENRRISIIVMKKGAENKIKVNK"
     gene            complement(32825..33730)
                     /gene="motA"
                     /locus_tag="U6309_000642"
     CDS             complement(32825..33730)
                     /gene="motA"
                     /locus_tag="U6309_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEN5504097.1"
                     /translation="MLIIVGYIVILLCVFGGFSLTGGHLAAIFQPVELLIIGGAAIGS
                     FIVGNNITVLKSVLKALPATFRGQKSAKQASSDLLSLLYHLLSKARQQGLMSLENDID
                     EPDKSPIFTSYPNLMSNHHLIEFICDYLRLIITSNMQPFQLESLMDMEIESHHEEEMI
                     PANAITKLADAMPAFGIVAAVLGVVHTMESISLPPSELGVLIAHALVGTFLGILIGYG
                     FVGPIATAMEQNANQNQLMLQCIKVTILASLHNNPPIIATEFGRKVLFTSQRPSFNQL
                     NDEIKNAKTAQTTTQNSEATEATSS"
     gene            complement(33760..34920)
                     /locus_tag="U6309_000643"
     CDS             complement(33760..34920)
                     /locus_tag="U6309_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN5504098.1"
                     /translation="MTRTKKSARQIEYHHIACSFQGGGALGAYQAGVLHALNEVGYHP
                     HWYVGTSIGAINAAIAAGNSDKERIEKMYQFWESIATPSAVNESTLLDDKISRKIQHL
                     IAAHSALLLGQPGFFVPRFPSPDFGWHNSPDLLSYYDTSPLHNSLEKFIDFDRLNNNK
                     LTRISVGAVEVSSGMMTYFDSYKQKIGPEHIMASGALPPGFPAVKVDGKYYWDGGISS
                     NSPVNYILNHNNQKQLLCFMIHLFDSFGMVPTSMDEVLKRKKDIEFSSRFIKLINLHK
                     EIHDLRNTIHILSNHISPEEKSKPEIRECIKKGRPSAISLVRFLYEHDETELSSKDYE
                     FSKKSIHERIQKGYDDGKNAIKKSPWNQSVPMTEGIALYDMSAKDTMKKYQD"
     gene            complement(34927..35709)
                     /locus_tag="U6309_000644"
     CDS             complement(34927..35709)
                     /locus_tag="U6309_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEN5504099.1"
                     /translation="MKLKNKVAIVTGAASGIGKEIALVYAKEGAKVAIADLNLDQASH
                     VAEEIKSSGGQAMAVAMNVTDEAQVNQGVDKVADHFGGVDVLVSNAGIQIIESVDKLA
                     FSDWKRMLAIHLDGAFLTTKACLKYMYASGNGGSIIYMGSVHSKEASVLKAPYVTAKH
                     GLLGLCKVVAKEGGIHKVRANVICPGFVRTPLVDKQIPEQAKELGISEEDVIKKVMLK
                     ETVDGEFTTTDDVAQTALFFASFESNALTGQSLVVSHGWFME"
BASE COUNT        10624 a         6422 c         7523 g        11366 t
ORIGIN      
        1 ttagtatatc aggatttgta cgttcaaacc ttcattgtgt agaccatgag agtttcgtaa
       61 actctatttt aaagtttaga gagaggatta caaattaatt gaatcggtaa aaaatgattg
      121 ctgtataata agcaaggttt atactttatg ttttaagctt taattaaaca gagagtcctc
      181 atggaattga gaattgataa tacccctttt aaagccattt ttcagccttt ggatctgggc
      241 tttacccaaa ttaagaatcg tttattgatg ggttcaatgc acactggact tgaggaagat
      301 aaagaaaatt taaatcgcct ggcaagcttt tatagagaaa gggctcaggg tggagttgga
      361 ttaatcgtaa cgggtggttt tgctcctaac cgagctggcc gtctggctcc cttcgctgcc
      421 aagctgacta atagtaaaga gcaacatcgg catgaggtca ttactcaagc ggtccatgaa
      481 gcaggtggaa aaattgcctt gcaaatattg catgcagggc gttatggtta tcaccctttt
      541 attgtagcac caagcagcat taaatccccc attagcccgt ttaaaccctg ggaaatgagt
      601 aaaagacgaa ttatcaaaac catcaaacat tttgcccgat gtgccagact ggctcaattg
      661 gctggctatg atggggttga gatcatggga agcgaaggat atttgataaa ccagtttata
      721 gtcactcata ccaatcatcg aagggatgaa tggggaggaa gtttctccaa tcgcattcga
      781 tttcctattg aaatagttcg cagtgttcgt gaggcggtag gcgagcaatt tattatcatt
      841 tatcgcttgt ctatgcttga tttagtggct gaggggagta attggcagga agtggttacc
      901 ctcgctaaag ccattgagca agctggtgcc agtttaatca atacgggcat aggttggcat
      961 gaagcaagaa ttccaacgat aggaacgatg gttccatctg ctgccttcac tcaagtgacc
     1021 cagcatttaa agccggaaat taaaatacct gtaattacat ctaaccgaat taatacacca
     1081 gaattggcta atcaattatt agaagatggg gtcgcagata tgatctctat ggccagacca
     1141 tttctagcgg acccctgctt tgcagaaaaa gcaaaattag gcgaaagcaa agcgattaat
     1201 gtatgcatcg cctgtaatca agcatgcctt gatcaagttt ttgtgaacaa aacagcatct
     1261 tgcctggtta atccccgcgc atgcaacgag acaaccttgg tttatgaaac aacaaatcaa
     1321 ccgaaagcta ttgctgtagt tggttctggg ccagctggcc ttgcttttgc tgtaacagca
     1381 gctgagcgag ggcataaaat tactctattt gaaaaagcaa atcagattgg cgggcagttt
     1441 aatttagcga aaaaaattcc tggaaaagaa gagtttcagc atactttgaa ttattttgct
     1501 taccagctcc aaaagtataa agtggatgta cgtttgaata ctgaagctac tgtggaatta
     1561 cttaaagact atgatgaagt ggtcttggcc agcggaatta tacccagagt tcccgaaatt
     1621 aaaggcatag atcataaaaa agtggtgagt tatattgatg tgattactca aaataaaccc
     1681 gttggctcac gagttgctgt cattggggca ggaggaattg gttttgatgt cgctgagttt
     1741 ttaactcatg atcatcaatt gacttctgag caattttata atgaatgggg aattgatatt
     1801 agcgggcagc atagaggtgg attaaaacca cctgaaattg caccgagccc tcgtgaagtg
     1861 tatttattac aacgcaaaaa agaaaaaatg ggtaaacgat tgggaaaaac taccggctgg
     1921 attcatcgct ccagtttaaa acataaacaa gtcaaaatgt tttcgggtgt aaattacgag
     1981 cgtattgatg atcagggatt acacttgact attgacgaca agcctcaagt tttggaagtt
     2041 gattctatag tgatttgtgc aggccaaata gaattaaaga atttgtatga gcctctgaaa
     2101 atggcgggca agttggtcca tttaatagga ggcgcttata aagctttgga gcttgacgca
     2161 cgtcatgcta ttgatcaggc ttgtcggttg gctgcattag tatgagtaaa attgctgtag
     2221 tgataaatcc tatagcaggc ggcggtcgcg gaaaaaaaat ttggaaagtc ttaaaacccg
     2281 ggttacatgc cctattcgaa caaactgttt acagaatatc taacagggtt aatgatcttg
     2341 aagcaatcac agacggactt cttgctgaaa atcctgacag cctgttaata atcggtggag
     2401 atggcacttt aaatcatgtt ttgaatggct taattgagaa cgataaatta aaaaatccgc
     2461 aaacaaaaat cgcctttttt aacgcaggtt gtggcggtga ttttacaaga caatttcccc
     2521 aacagaaaat gaccgaattc ctggacagat taagtcataa ccaatttatc aagtgtgata
     2581 ttgggaaaat tactcttgcc aatcaagtat cgaggtattt tattaatatt gccagctgtg
     2641 gtctgtcagg acatgtggtt ttgcgcgtat ctaaaagcaa gtggttaaaa aaactgggcg
     2701 gtactctgaa ttacttgttg catgctttaa ccgggttgat gacctatcgt aaaaccaaag
     2761 taagggttca gatagacgat gctctgccat ttgatagctc catcctgctc atggccgtct
     2821 gcagtggcca gtatttcggc ggaggtatgc atgtagcacc aatggcaaaa gttaacgatg
     2881 gcttgtttga tgtggtttct tttaatgaat tttcaaaact aaatgcactt ttaaaattat
     2941 ataaaattta ttcaggaggg catttgttgg ataataatgt ccattatgtt caggctaaaa
     3001 aaattaaaat agagccgttg gaagaaagta aaatagaaat tgaggcagat ggtgaaatta
     3061 ttggttgctt accggcacag tttgagttaa ttaaagaaac tcttcctctt attgtttaaa
     3121 aataaaggtc aaaatagggc tcattctata aaattattat ttatcaagac aggttgttgc
     3181 ctcaagacga gatcacatta ttaagtgcaa taaacaacac aaacaattgt ttatattttg
     3241 tcatcctgcg caggcgggaa gccattttta ttgtaagctg tggagcaaaa tttaaaatag
     3301 attttcactt gcgtgggaat gacacatatg atgtgttatt tagtcaaaat atgattttgc
     3361 cctggttgtt gtcttgataa aattaataaa tatagtgcat catcttgtta attttgttat
     3421 tataatgcac attttttata ttagttagcc ttttcagaaa gacgctttat tatattcagg
     3481 aacagtatga atcaagaaat atcaaacttc tctactttta atttttctag tgctttaaat
     3541 aaagcgttag aagatatgaa gtttattacg ccatcgccta tccaggctca aaccattcct
     3601 ttgatattgg aaggtcgtga tgccattgcc cttgcgcaaa ctggtacagg taaaacagca
     3661 gcttttgcat taccaatttt gcaaaatctg tctcctgaga tatctaccac tcaggctttg
     3721 attttagccc ctactcgtga attggcaatc caggttgcag agcagtttga attattaagc
     3781 aagtaccaac gcaatgtgac gattgctgtt ctttgcggtg ggcaagaata tggtcgtcaa
     3841 ttaaaacaat tgcgtagtgg tgcgcaagtg gttgttggaa cgccaggacg tattctggat
     3901 catatcgata aaggcacctt gctcttaaat aatctgaaga catttattct tgacgaagca
     3961 gatgaaatgt tgcgtatggg tttcatcgaa gatgtagaaa ccattttaga aaaactgcct
     4021 gaaaaaaagc aaatggcatt gttttcagca accatgccct atcgaattcg tcaaatagcc
     4081 aatacttatt tgaatgaccc tgcctcaatt gaaatccgta tggaaactgc gacggtcaag
     4141 tctattgagc aacggttttt atttgcttcc gtacatcaaa aaccggatgc gttaatccgg
     4201 gtcttggaag tggaggatta tcaaggggtt attgtttttg tacgcactaa aagcagtact
     4261 gaggaagtag cagaactatt gcaacaatat ggattaagag caatggcaat tcatggtgat
     4321 attactcagt ctttgcgtga gcggattatt gcccaattta aacaaggagc gattgatatt
     4381 ctggtggcga cggatgtcgc tgcaagaggt ttggatgtcg agcgagttac tcatgtgatt
     4441 aattatgata tgccacatga caatgaaact tatgttcaca gaattggcag aacagggagg
     4501 gctggccgtt ctggtgttac gattttattt gtaactccca aggagtcgcg tttaatttcc
     4561 agcattgaac gccataccag gcaacgaatt gagaaagttc aagttcctaa tgaccatatg
     4621 atacagatgg ctcgtcaaca aagattcatg tctaacatta ctactcgact gaatcatgag
     4681 catgtgtatt cctacaaaag gattgtggaa gaatacatta aagagcatga cgtatcagca
     4741 gtagatgttg cggctgctct tgctttattg ctgcataaag atatgccttg gagaaaagaa
     4801 ttaaatatgc ctaaagcagc ttcagcattc aaagaagaga gagctcgggc aggagaaaaa
     4861 ggattggatt tacgtggtcg atttaaggat gatagaaaaa gttcttttgc tgaccggaaa
     4921 ggtgcgcgag gaagcaagaa agattttgcc aaagattttt ctaatgaaag gaaaaaatct
     4981 aatgatggca ggaaggccgt taatcaagaa tcagtagccc aggatttatt tcgtatcgat
     5041 gtaggcaaaa ttcatggggt caaacctggc aacatagtag gggctattgc taatgaggct
     5101 ggcttgcaaa gccgatatat tactgggctt aaaattcatg aagatcactc cactgttcgt
     5161 ttaccaaaag gtatgcctaa agaagtgatg aaggacttaa ccaaggcttg ggtttgcggc
     5221 cgccaactca atttaacatt aattggtagt gcctgaatag ctcagcagta aagcgctgtt
     5281 agcgttatca attggccttt aattattttg caaaacgatt tcgcctgtcc agggcgagat
     5341 cataacatta agtacaataa atagcgcaaa caatggtgta cattttatta tccccgcaca
     5401 ggaaggaagc tatctcttct ctatagcaaa cggaggggca agatttgaaa tagattctca
     5461 cttgtgtggg aatgatacgc atgatgtatt atttaatcaa agtatgattt taccctgaag
     5521 acctaccttt ttcctggctt gtttttctaa tttaatacct ctaatatgga ttggtgttct
     5581 gaaatttaac actgaggggc caaattatga aaaagaaaca tgaacccggt tttttcggta
     5641 gagaaaccaa gaatatcaaa gacattgaaa gcaatagata cgatcctgat tgcccaattc
     5701 ggagagctga taagtctcgt ggattcagcc aaaaagcaag cggccagttt cagctacgaa
     5761 taaaagaaga agtaaaagaa gcgttagacg acattcgtaa tgaatctgcc tataaattta
     5821 attaaccatt attttatctc tgagtgtaag agatcagctc ctgcttgaaa cgaccgtatt
     5881 tggtccatct tgaacggaaa taaccattgg agcactgatc tgtatatttc tgagtttaat
     5941 gaagcctgac aagaggcttc attagtaata aagcactacc tttatggttt gttgccaaat
     6001 aacactgatg cagcagtagc tgctacggct acagtggcag caacgaatgg gaaaagaaca
     6061 taggggtttt caaactgact tttataaggt acttcgtctt tgagtttggc aattaattct
     6121 tttcttatct gactactatt ttctctcaca gattttaatc tattctcagg gtcggtgttc
     6181 gctgtaagta ttggaacata gtcatccaat aatatgtttc tggcactttg tatttgctct
     6241 gtagtctcac tggttggatt ttcatatttc tccgaaacaa gagctatggc ttctgcgatt
     6301 gcagaagtga attttagctt atcttctttg gaccagtcat cagcatttgt tatacgtgaa
     6361 ttaatttgat agcgggggtt ttcatattgg ccttgagtaa aatggtgaat tgcatcatcc
     6421 agcttcttgt ccacagagag tatggtttca agaaacatca agctattaat caggaaattt
     6481 ctttcaaatc tagtcattat tatggttctc ataaagaaat aagggctgac gacatttctg
     6541 ctgaatcgat tgcaagatat tgattttctg taccctgtta caaaagaaag cattatgaat
     6601 cgagtcaggg aagtgtttac aggatttact ataattaggt ttctgtttta gatcaacaaa
     6661 aaatttatat acagtaatgt gttaaatgcc gtggaaaaat tgataaatca ttatataatt
     6721 gaatgaacct aaagatataa aatcaatcta ttatttaagg ggtttcatga aactattagc
     6781 atctttgtgt gcttttttat tgtcgggagt tgttagcgcg acaccactta ataacatagt
     6841 tgtatttggt gatagtttgt cggataacgg taacttatat gaatacatga aacaccaact
     6901 tcctcaatcc cccccttatt ttgaaggtcg tttttccaat ggcccagtat ggatagaacg
     6961 tctggcagct tcatacttcc caaatgatcc caatactcat ttgctggatt atgcttttgg
     7021 tggggcagga gtctctgtcg atgaagaaga tgatgaagta ttttttacct taagaagaga
     7081 agtgaattct tatttgttag cacatcagga taaagcaagt cctgacagtc tttttgttat
     7141 ttggataggg gcaaataatt acctcggtat gcctgttgaa gtggaagaaa cgcttaaaaa
     7201 tgttaaccga gggattacgg atagcataca acgtttggta gacaagggtg cgaagcatat
     7261 tttagttctc aatttgcctg atttgggaag aactcctgcc gccctggagt ttggctctgt
     7321 tgaagagatg acctatttct caacgcagca taataatgca ttatccaata cagtcgatta
     7381 ttttaaaaaa acttatcctg aagttgagtg gttgtttttt gatacgggta gtcattttga
     7441 ctatgtgata gaacatgctt cagagtatgg atttacaaat ataacaggta cctgctcctt
     7501 ttcgatagta gatgagatta ccaaaaactc cgttttgaag atggttgcat ctgtgaagcc
     7561 agaattgaca gaaagcgcat gtgatggtta tttgtttttt gatttggttc atccgacagc
     7621 gttggcacat aaaataatgg cagaaaaggc gcgcttgatg ttggatgaag ctggtgtgga
     7681 gttcgccgag aattaagagt tttttatggc gatagcaaat gaaacttgca tcgtcattca
     7741 aatgaatccg caatgataag agcggggatt ttatactgag cagaacccaa tgagagagtg
     7801 gtgtatttga acttcacaaa tattctctca ttctaattgg cactagctag tgcgtcttgt
     7861 ccatcatagg caattttata gggaaatatg aatcaaatta aatggataat gctactattt
     7921 tgttttgttg tttccaatgt tttcgcggat aaacttgata taaccgtaga tcaaaaaaaa
     7981 atcatattgc cctattggtt atcaagatct gtttcctatg gggcggttat cattgttagt
     8041 ggtggagaga cagcgcaatg gtccttatta ttagaacagt ttgccaaggg attgtctcga
     8101 aatggttggt cagtcgcatt attgaattgc actaaaaata actcgatacc ctggattaat
     8161 caattaccgg aagtcatcag tactttaagg cagaataata ataaaagaat tgttttagtc
     8221 cattatggtg atcaacttaa tctggcatta gagtatttta gcaagcccca atccaagatg
     8281 atcaatgggc tagtgatgtt gtctgcctat gatttaaata gaaatttgca aaaagtacct
     8341 cggttgcgtt ttcccctgtt tgatattgtg gggcagtttg attatgacat ggtaaggcag
     8401 caacgaaaga gcagggaaga aaaatttaaa gagaatagct atttagccat tgatatacct
     8461 ggcgctactc atgactacca atacagtcaa caattattat tggcgtttgt tcatggttgg
     8521 atggcaaagc ttccagaatt tgagcctcag ccaccgccga tattggtttc ttatttggaa
     8581 ccggtttatt ctacagcaag ttcagtcgta ccgaccaaca aattggacta ccagcccttg
     8641 gtttcacaga tcaattcata ggctttcata atttgatgag ttttatcgtt agccaattta
     8701 atcatttctt cgggtaaacc ttgagcaatt aatttatctg gatggttacg gctcaatagg
     8761 cgtctgtacg ctcttctaac ctcctgtttg cttgcattcg gacttacctc aagcaatgca
     8821 taagcgtgcg ctaaagtatt agggggtgat tgaggtttgt aaccctgttg atcttgagaa
     8881 gaggaggtat gcttgtaccc ctgttgatta tgatagtgtt gtttagattg ttcctgctgg
     8941 aaataagagc caaaatcctc ataaaaacga tactgcttgt gtaagggagc aaatcccaga
     9001 tgcgcaaaaa tgttatctag cgcatgaatt ttttggctac ttaatccatc tacttgagct
     9061 gccctatact gaatatctac aaaaagcttt aacaaatcac ggttatcctt acagattctt
     9121 tgtaattgga taagagcaag actgacatta aaatcaggtt gtttcccttc attaaataat
     9181 cgttttgcca aatctttttg ccccttgctc aatttcattt cattcatgat ggatttagcc
     9241 atgctaatct cttgttcaga aacacggcca tccgatttcg caacatgccc cattatggag
     9301 aaagtggctt caaagaaagc tttttgaacg attctttgtt tttctgcatg gtacagccaa
     9361 tggggattag agtaataact gacaaggccc cgatcaaaaa agtttccaac gagaatgcca
     9421 aacaaagcgc ctactggtcc agcagttaag tagccaaaga aggcaccaag tatttttccc
     9481 caccaggttg ttattacaaa gaaatcgcgt aagttcatta tcagaaatta cctataaaga
     9541 tatgtgttaa atttaacttc aagttctaat aatatagatt gaggacaaca tagtctctaa
     9601 ttgaattgta acatatcgca caaggaactg ttttaggtat atactttacc tttttgtgtt
     9661 tggtgcctgt ttatgcgttt tgtgtattcc tttttaatgt acttactgac tccttacttg
     9721 ctttttcgcc tgtggcggaa aggtagaaag ttgccagctt acaggcaaag gataggagag
     9781 cgattttgtc tgggaataca agaaaatgca ccagtcgatg tatggcttca tgcggtttct
     9841 ctgggcgaag tgatcgcggc aacaccttta attgatgcaa tgctcaacaa acgttggtca
     9901 gtgttagtca caacaatgac tcctacaggt tctgaacgag taaaatctcg atttggtcat
     9961 aaggtagcgc atcaatattt accctatgat ttaccttggg ttctcaaacg attttttaaa
    10021 agaacgagac ctcgtgtcgg tatcattatg gaaactgaat tgtggcctaa tttaattaac
    10081 caggctcaag cttcaggagt ggcattattt ctggcgaatg ggcgcttatc tgatcgctcc
    10141 ttgcaaggat atctaaagct caaattttta tttaaaccgg ttttgaatca attttcaggt
    10201 attttgactc aaagcaatga agatgctgag agattcattg ccttgggtgc aaatgccgat
    10261 ttagtccatg tattagggaa tatgaagttt gatttacaga ttaactctgt tgatcgtagt
    10321 caatacaggg aattaaaaag tcattgggga gaggatagac ctaccatcat tgcagcaagt
    10381 actcatgacg acgaggagtc tcagatttta tctcagctac ctcgtttgca ggaagctatt
    10441 ccgggagtgg tgttattaat tgctccgcgt catccagaac ggtttcaaac ggtctatcag
    10501 ttgagtgttc aagcaggatt taatactggg tgtcgtagta atttgaatac gatttccaga
    10561 gaaaatgaag tcgttatact ggacagcttg ggcgagcttt tgggatttta tcaaattagt
    10621 gattttgctt ttgttggcgg cagccttgtc ccggttggtg gtcataatgt attggagcca
    10681 atcgcgatga atgtgcctgt tttaagtggg aatcaagtgc ataactttaa atcaatttgc
    10741 cgagagctta aagaggccca ggcaattttg ctagtgaatc atgccaatga gttggttgat
    10801 gcaattatca agttatatca ggatagggaa tctcaaaaca ctatggttgc aaacgccagt
    10861 tcagtccttg aaagtaataa gggttctgta gtgaggtatt tacagaaaat tgagtcagct
    10921 ttgggatatt agcttgttat aaagttctct ctatgttgat ttcttgtatc ataccaaaaa
    10981 attcatcaaa acatttttga acttgttcaa ggtggagtat ccatggacaa tggcctgctt
    11041 tataaatgac tttattaatc acattttgac attgaaaatt cttatcctga ataaatattg
    11101 caggtggaca aatgtaatca agttcactgg caatggtcat ggttggaatg ctttgaggat
    11161 gccacttata gtgaaaatca ttataaaaat gctcgatggc ataatgatag gattcattgt
    11221 tgtaggcaaa taattccatc atttgctcac cgattggtag ctcttcctga gtaaaacaat
    11281 aatgtttata agtatcccaa aattttttat aggtttcgtt ggatggatat aaatgatatt
    11341 ggcttgcagc agggactaaa tccggcaaat tatgtttttg acgcatttcg ttaacatgct
    11401 gaaagaagct gttggttgtt gttgtattca ttaaaacaag cccgcttaaa tgattctcaa
    11461 tttccggaaa attcagcgtg aacattcctg aaaaactatg ggtaacaagg attggcttgt
    11521 tcaaagattt cagtaatgaa atgaggcctt tctgccaaga tacaaaatca agttggcctt
    11581 caccattatt tccatctttt ggaaaatcaa caaggcttac agtgccctgc aaagttagct
    11641 tgtcgcaaaa actggctaaa tattcagaac caagtcccgg tccacctggc agaaacagcc
    11701 agtgttgatc cgagcctttt ttgatttgat ttaatctgta gccataattg gtaaaataca
    11761 taaaaaacat cgattgcttg taataataaa ctcccgcctg gcgggagttt attgtctaat
    11821 gttcagaggc aaggaagaat caagctccct gattttgtcg ttccttgatt tcagataggg
    11881 ttttacaatc tatgcataag gtcgcagtag gtcttgcttc aagacgtttc aggccaattt
    11941 caattccaca ggcttcacaa tatccaaaat cttcatttct taatcgctca agagcgtctt
    12001 cgattttttt aatgagtttt cgttccctgt cgcgggttct cagctcaatg ctaaattctt
    12061 cttcctggct tgctctgtcg gaaggatctg ggaagttggc tgcttcatct ttcatgtgcg
    12121 agacagtacg atctacttct tccattaaag attggcgcca ggcgagcaat attttttcta
    12181 tatgtgccag ctgtttttca ttcatatatt cctcgccctc agtctcttgg tagggcgcta
    12241 ttcccatatt tcctattgca tcgtttttca cgttgtataa tctctctttg gttttatcaa
    12301 ttaattgtcc tgtcatgcca gaccctatat ttcattctgt atctgaaact gagttttata
    12361 caaaatcgct acgtatacca aaaaacgtca gtatcatcaa caaaaaaata atcactacaa
    12421 aggtttgtta ttccgattgc ttggacgttg tcctccactt accctatcat gtccttgaag
    12481 atcttgccaa cagtgtatgt ttttatatcc taattgatta agtatagcgc ttatctcatt
    12541 tttttgttca aaaccatgtt caaccaagag cagtccatca ggcaaaagat attcataact
    12601 gtgctgaata atatattgta gatcagctaa tccatcttga ctactgacta atgcacttgt
    12661 tggttcaaag cggacatcgc cttgttttaa atgaggatca ttttcagcaa tgtagggcgg
    12721 attagataca atagcgtggt attgttttag ggggagatta ttaaaccagt aggaatgata
    12781 gaaattaata ttgtttaagc cgagcgtttt agcgttataa cgggctaact ccaatgcctc
    12841 tttgctgaaa tcacaggcgt caatatgcca aagaggtctt tcttttgcaa gcgccagggc
    12901 aatagcacca cttcctgttc ccagatcaag gacagaaata ttctccttgt caggtattaa
    12961 ctccagtgca agttccacca gacgctcggt ttcatgtctt ggaatgagaa cattgggtgt
    13021 tacttttaac gaaagagacc aaaattcgcg ctgtccagta atgtaggcga ttggtagacc
    13081 ttcagcacgt tgcttaatca tctgcaaata ggtttctatc tgttcaggag aaaccaaagc
    13141 atccggatgg gcaaaaagat aggctcggtt tttgtttaac acatggcaga gtaaaagttc
    13201 tgcctctaac tttgtttcct ggtttttgac tttgagttgt tgcagagctt gttccagtaa
    13261 tgaacgaata tcaatcatgt cgacctaaat cggctagcag ttcagcatga tattcgcgtt
    13321 ttaacgggtc aatgaccagg gataaattcc cttccatgat gtcactaagc tggtagatag
    13381 tcagattgat acgatgatca gtgagccgac cttgaggaaa gttataagtt ctaatgcgct
    13441 cggagcggtc acctgtacca accagtgact tacgtgtttg agcttgttct tgtttttgtt
    13501 tgcttacttc ggcatctaat aaccttgttt ttaacaagga cattgctttg gcacggtttt
    13561 tgtgctgtga acgctcatcc tggcactcta caaccactcc tgtagggatg tgtgtgatac
    13621 gaatggcaga atctgttttg ttaacatgct gtccaccggc tccagaagat ctgtaagtat
    13681 cgatgcgtaa gtcatcggga tttatctgaa tgtcattaat ttcatccacc tcaggcatga
    13741 tagcaaccgt gcatgcggaa gtatggacac gaccttgcga ttcagtttca ggtactctct
    13801 gtactctgtg agctcctgat tcaaatttga gttgtgaata aaccgcttgc ccactgattt
    13861 tggcaattat ttccttgtag cctccatgtt caccatggct ggcgctaatt aattcaattt
    13921 gccaaccctg gctttctgca taacggctgt acattcgaaa gagatccccg gcaaatatag
    13981 ctgcttcgtc tccacctgtg ccggctctga cttcaagata aatattgcgt tcatcatcag
    14041 gatctttagg aatgagatgc cattgtaatt gttcatctaa ttcctcaatt tgtttcttga
    14101 cggtgtctat ctcttcttca gccatagtcg ctaaatcttt atcatcactt tccaataatt
    14161 cgtttaaaga atccaaatta ttttttgcct ctaaatacga ctcataacat tgggaaactg
    14221 gttcaagttg ggcatattct tttgataaag acttaaattg attttgatcg gcaataatgg
    14281 atgcttcgga cagtaagcgg cctacttcct cgtatcgctc caacatttgc tgtaatttta
    14341 actcaagaga tttcttcata taatgattga ttggctgttg tatcaaagag atatcgtgcg
    14401 aggtcaagta agtcttctct gttatcccat gccatttgtc ttaaacctat ggtgggatta
    14461 tgagttagct tgttgaccaa gcgcatactg aattcattta aaacgtcttg ctgattttga
    14521 ccagcggata tttttttcaa agcacgttgt aattcctgtt gagccaaagt gtgcatttga
    14581 ttgcggtaat cacaaatgac gtctttggct ctaagcgagc gatgccatct aatgtagtta
    14641 tccagttcgc tttcgatcaa ttgttctgct tgtaatgccg catttcgcct ttcatccatc
    14701 cctttttcta tcatagattg taaatcatct acattataga gatgaacttg ctccaattca
    14761 ttgacatttc cttcaatatc acgaggaacg gccaaatcca ataaaaacat aggggcatgg
    14821 tttctttgct ctaaggcatg ctcaaccagg cttttattga tgaatgggag tgggcatgct
    14881 gtggcagaaa taacaacatc ggccagaggt aaatattgcg ggatatcacc aatggacagt
    14941 gtttttccac cgaaagtttc agctaatttt tgagcatttt ccagagtacg gctcgctatc
    15001 aaaaaacgat ggacaccatg ctgatgaagg tatttagcaa ccagagaggc cgtttctcca
    15061 gaaccaatta aaaagacact taaggaatga tagtttttaa ataattgccc tatcagttgt
    15121 accgcagcat aggcaatcga tacgggatta gctccaattc cgctacgagt tctgatgcgt
    15181 ttggacgctc tgaaaatata ttcaaataca ggccgtaatt gtgtttttac tgtgccaaga
    15241 cgacaagcat gttgatacgc ctgtttcatt tggccaagga tttgtggttc gcctatcatc
    15301 atggaatcca aaccgctggc aacacgcaag gtatgtttga ttccttcttt tccctgatgg
    15361 atatacaaaa attgagataa taattcctct gataattggt gttcatgagc taaccagttt
    15421 ccaagaacct caggggtatt ggtatcacag tagatttcag tccggttgca tgtagataaa
    15481 atagctgctt cattgacttc aggtaaatca agcagactgc tcaatagaga atcttgcatg
    15541 gctggctgca gtgcgacttt ttcacgcaca tgaattggag cagttttatg attaagcccg
    15601 caagctacaa ataccataga atcttgattt atttgtttaa gggaattaac gaattgtaaa
    15661 cgattcattg taaaatttgt agagaaaaag gcaaggttcc ttgtatttct gtttcttgca
    15721 atcattttac agtatatttt ttacccgtct tcttcctcaa gattgtcccg cttgagaaaa
    15781 tttgctggca agggattcaa tttactttct ctatcttcaa acgggctcat atcaaaataa
    15841 tgagagcagt gactttctaa agtcctgttg agttttgccg ccaacatgta tagccacata
    15901 ttgcacaaca tgtgtcattt caataggagc tttaaatgga cattatcctg caaggtcaac
    15961 ataatgctat tgaaactgtt gagagcctgg agagggttct gaaattattt aaagagcgtt
    16021 accaggttga tggatttcgc gagattcatt tgactgtcac tttagttgat gaacagggcg
    16081 atgaggtgga gttggttgat agtgagacga atcaacctta tcgaattttt gaggtttatc
    16141 gtaaaggata tgaattggct ggaagaaaag ggattccaat gttgcagctg gtagttgata
    16201 atacgcacaa atagattatt ctttctggtg cttttttaat aagcctcgta ttctggataa
    16261 ggagtccatt atgattatgg cattggtagc cataatactt acgttaatcc tggtgatagg
    16321 tgtacatgaa ctggggcacg cactgacagc ccgcctgttt caggtcaaaa ttagtaaaat
    16381 ttccatagga tttggaaaac cattaattaa atggcaaacc caaagtggtt gtaactggat
    16441 ctgggcaatg tggcctttgg gagggtatgt tcaattactg aattcgcgga ttagtccagt
    16501 aaagccacaa gaaaatgctt attgttttga taagaagcca atttggattc gtgtgctgat
    16561 tttattgtca ggagcgattg ccaaccttat taccgcatgg atagccttgg tgttggtttt
    16621 ttatattggc attagctata aattacctca aattcaatcg gtaaaactgg atagccttgc
    16681 tgctaaggcg ggtattcaag caggggacca atgggttgct gtagagaatt acccaactga
    16741 ctcctggcaa ggggtaggta tgcaattggt aattcattgg gggcaaaagg atgttcacat
    16801 tgttttaagg caagcgaatc aacaattaaa acaattgaat cttgatttaa gtcagattga
    16861 atttacttca aaagatggct ctctgctcga gagtctaggg atcaaacctg acttgtcagc
    16921 agtttccagt ttgacgcgct atccatctct actggcttca atgcaaaaag cgtttgctga
    16981 gataatccat accatgtact tttttataat gatattgaag caattgtttc ttggggttat
    17041 ccctttttca attttacttg gaccgttggc tattttttct gtttctgtag cttctttaac
    17101 tcaaggcgtt atcgtttttt tactattcat tgctacattg agtcttgctg tagctttggt
    17161 caatttattt cctatacctg gccttgatgg aggcagcatt ttatattcgg ttatagagaa
    17221 aattcgtggg aaacctgtct cagtggctgt ggaagtcttg cttcatcgct tgatgattat
    17281 tctattttgt gttttgttag tgcatttatt aatgaatgat ttgaatcgat atttacacca
    17341 ttgacagtgg actcatagga acgggctgcc ttttaattct tagttacagc ccgcgcacaa
    17401 caacatatat ctactctctt aaccccggca ttttttaatg tatgtgccat ttcattggct
    17461 gtgctgcctg tggttaataa atcatcaaca atcatgacgt gttcataagt tacaggtgat
    17521 acataaaaag catgacgtaa attttttctt cgttgctctc catcaaggtt agcttgtgag
    17581 tcggtattga ttattttttg gcaataatgt aaatcataag ggatttttaa ttgacgagct
    17641 aataatctgg ttaatacagc agcctggtta aatccccttc gttttaatcg tttgggatac
    17701 atgggaacgg gaattagaca atctggtttc aatgcgcttt tgggtaaggc attaagaagt
    17761 aattgcttta gaaacgaggc cagataaagg ccattatggt atttaaactg atgtattaaa
    17821 ctacgcagag gctcttcaaa acgataggct atataagcac tatcaaagga gggccttttt
    17881 ctaacacatt ggccacaaac cagatacgta tcatcagaaa gggggtaggc acagtactgg
    17941 cagcaagatc caagttgttt cataaactcc atgcaattag agcaaactgc aagttggctt
    18001 ttgtggaact ggttgcaaag agtgcatatt gaaggtaaac gtaagttctg tgttatactt
    18061 aagaattttt ggagcacgat agttggattt ccagcattta atttttccct ggtctttata
    18121 atgacggtca acaatgaaat aagcaaggca tttagcaaac atgccgatga ctatgagcgt
    18181 gtggccaaag ttcaaaaaga aatcggcagt cgtttgtttg agcgattaca atatttgaaa
    18241 attgctcctc gacgaattct tgatttgggg tgtgggcctg gatttttttc aaaagaattg
    18301 gctttgttat atccgaaagc gcagattgtt ggaatggatt tgtcgtttgc catgttagag
    18361 caagccagga aaaaacaggg ttggcgtcgt aaatggcctt tagtcagtgc cgatatgcag
    18421 aaaatgcctt ttgcaacagg cgcttttgat ttggtttttg ctaaccaggt tattcactgg
    18481 agtagctcat taggcacagt gtttcgggaa cttaatcgtg taatgaatgt caacggatgc
    18541 ttgatgttta ctaccttggg tcctgatacc ttcaaggaat tacaaacggc ctggtctgcc
    18601 gctaatcaat atgctcatgt taatgaattt gttgatatgc atgatattgg agattgtctg
    18661 attgctgagc atttcatgga cccggtcatt gacatggaat tactgtctat acattacgag
    18721 acccttcccc aattgctatt agctctaaaa actcaagggg taagaaatat taaccctaaa
    18781 cgaaatcacg ggctcacagg taaaagtgca tggaaacaat ttgaagcaca atatgctact
    18841 atgcggacta ctaccggcaa atatccctta acttatgaag tggtttatgg gcaagcctgg
    18901 aagggtgcgc aacgtaaaat ggatcaaggt atagaaacct ggattcctgt ttcacgtgtt
    18961 gttaaaaagt cctaatttaa aacggatatc aattggattg tccggtgcat caatggtgtc
    19021 aagacaaatt gctacgattt tgtaattgtt tggttaattc aattaatgca gccgctttac
    19081 taccaaataa ccgtagagaa tccattgcaa tttggtaatg aacagcgatt tcttcttcca
    19141 attgttgctt attaaataag gttgcgaagg tggttttttg gtttgcctga tccgaggagc
    19201 gaccttttcc caatatttga gttggagcgt aaagatccag gtaatcatct tgcatttgaa
    19261 atacaagccc tatatgtttc ccataagttc ttagtgcgga tttgatttgc tctgatacct
    19321 catgttgtgc tgctaaaacc atttcaaaac acgccaggat cagttttcca gttttcaaaa
    19381 gatgaatttc tcttaattgc tcctctgtta ctgatgattt ggccagttct gataaatcaa
    19441 gactttggcc actaaccatt ccacttattc cactggcatt taccaatact tgagtaatgg
    19501 ctacgacttg tgctgcgggg agaaggggcg ataatctcgt caatacgact tcaatcgcta
    19561 aagcttgcat gccatcgccg actaaaatag cggttgcctc atcaaatgct ttatgacaac
    19621 tcggcttacc gcggcgcaga tcgtcattgt ccatcgccgg aagatcatca tgaatcaggg
    19681 aatagcaatg tgttaactca agcgctgctg caataatatc tagaactccc tggtcaacat
    19741 caattaaatc gccagctaaa taaaccaaaa tgggtcttat tcttttgccg cctgaaaata
    19801 aagcgtaatg aagtgcagag cgaatttttg gagctggtat tattgtttca ttgagcaatt
    19861 gctcgagata tagctcatgt ctttgggtat acttatcaat cacttgtttg ttcatctgag
    19921 tcgagttcaa tgttggaatc agtaccagtt aacgtttcaa ttttttgctc tgcctggttc
    19981 aaagcatttt ggcaacgtct tgcaaggcta attccctttt caaattgttt cagcgattct
    20041 tcaagggtaa gctctccctt ttccagttgc cggacaattt cttcgagttc agtgattgat
    20101 tgttcgaaat gtatgccttt gctcataact gttttccaag gcgataaaag gtgtgattat
    20161 actgggttaa tgcgaggatt caacttctga atggaagtaa gtggaaaatc aatcatagaa
    20221 gcttattggc agctttgggt tttataaagg aactccagaa ctggatgttg gactttgcta
    20281 gtttaatatc aaagaccaac attaggtggt tagcagtttt taaagaccaa atgctttatc
    20341 aaaatccaga tcaaattctt cggtctttgt tttgctattc ctcgtagtga agaacgcggg
    20401 gctgtgtttc atggatgcat cttgttgttg aatctgatca cggcatttat cttttaactc
    20461 aagcatttca tcatataaag aacgtatttc aattttcaag cggtcaacta cttcactgcc
    20521 gcctttgtaa attgtggtta gagaacccat tttgacaaag tgttctgtgt gggcattttc
    20581 caggaatttt agttgagctg ttaacgagga ggactctttt gctttcgcta tttggtcttc
    20641 catatactct tttaatctgg taagttctgt taccggattt tgtccttttc tgaaagcagg
    20701 catgatattg acctcacatt ttaaaataat cccttaggta agttaaatca aaaacaggta
    20761 attgaaaagc aaatattttc atattctctt ttattagata agttttaatg catagtgctt
    20821 ggtatagggt tgatcaattt aaattgaagc catattgttt gtcagataag tctatggaaa
    20881 tcaggcgcga tttgtataaa aaacctgaca ttcgcgttta aacatcgtga agccaagcga
    20941 atgtcaggtt tttgtttgaa gtttaattat tggggggatg aagccgcttg acgaagtccc
    21001 aaatcttctt cagctttttc taccgctttc gctgaatctg gacgtgcata agcgaagaaa
    21061 ccttgttgat aaccaaacgt aaagcaggta atcaccgcgt tgcccaagca ctttaaaagg
    21121 tttttcaggt agtcacccca gcctaagtct ttttctaaaa ccggtttagc tatttttacg
    21181 gcatcttgac aagctaattt aaactttctt ccagcaagtt cctgagaaaa ttcattggct
    21241 cttaagtctc gctcatactc atcacgagca gctataagac tttggagcag tgtatttgct
    21301 gtgtcatgtg cttttttata ctggtgttga tcaactctgt ctattttgag tgtgagctct
    21361 tgaataattc catcaacttt ctggagagct tgagttatag tttcttcctg ttgattttca
    21421 agcttaggtt tttcttcttc agattgcttt gcaagtttta gcttttcttc ttcaatcttc
    21481 ttttctagct tcaatggttc ttcacgctta agcttttcta cttcagcctg tttgcgttgt
    21541 tcttctaatt ttgtttgaac ttcactaacc agtgttaatg gatgcttatg actgaattga
    21601 tcacgtaaag gctcataaag ttttcttacg tcaattttta gctgttcaac ttgctcattg
    21661 ccacctttgt aaacttttga tagggaaccc attttatcat cgtattcatt caaagtgtct
    21721 atgagcagtt gatcgatgtc ctgttctgtg gttgcttttt taatttttga aatttgctct
    21781 tccatgtatg ccttaagttc agttaaacct ttaactggat cctgacctac ggtgaatttt
    21841 gccataattt tatcctcggt ttttataaaa aaatcacttc ggcaaataat aaatcaagat
    21901 agtgtttttg aaaagtaaag ttttaataaa tatttcgtaa attgagtgca ataaaaagaa
    21961 aaagggggtg ttcaagataa ggtaagttct ggttcattta aaaattattt aaagcaaata
    22021 tgttcagcgc gagttatcat aaatgtacta aataaaatgc ttgaatcaag gcaaaaatta
    22081 tgaaagtgaa aacccagttt gtttgtaatc aatgcgcggc catttttaaa cagtgggcag
    22141 gacaatgcac tcaatgtggc gcctggaatt ccattgttga agaagggatt gtgcctgaaa
    22201 acaagacttc acgaagtggg caatgggcta atcagcgctc tgtcatcact gctgttgaag
    22261 acgtcgtcat ggacaaaata gtacgtatgg attgtggtat atccgagctc aaccgggttt
    22321 tgggaggggg gctggtttat ggctcggttg ttttgattgg aggagatccg gggatcggta
    22381 aatccacctt actgttgcaa acgctggcta atttatctat gcaagaaaca gtattgtatg
    22441 tgacggggga agagtcatta caacaagttg ccatgcgtgc ccaaagattg gggctacctt
    22501 tggccggatt gcggctattg gcagaaactc aggttgaatc catcattgct caagcccaaa
    22561 aggaaaaacc gcgaattatt gtcgtcgatt cagtacaaac gatattcact gaagccataa
    22621 gttctgctcc gggaggagtt agtcaagtca gggaatcagc agcacaattg gtgcgttatg
    22681 ccaagatgac caatacggcc atcttcctgg tagggcatgt caccaaggaa ggaacattag
    22741 ccgggcctcg ggtgttagaa catatggttg atagtgttct ctattttgag gggcaaaatg
    22801 atagtcgctt cagagtcatt cgcgccatta aaaatcgttt tggcgctgtc aatgaattag
    22861 gtgtatttgc gatgactgac aaaggcctga aggaggtagc caacccttca gctatttttt
    22921 tatctcgcca gcccgaacct acgcctggca gtgcagtgat ggtgacttgg gaaggatcaa
    22981 gacccatgtt ggtagaagta caagcattgg ttgatgaggc ccatggtcag caagcgaaac
    23041 gcgttactgc gggtcttgag cataatcgct tggccatttt gttagctgtt ttacaccgtc
    23101 atggaggtat cgctacttac gatcaggatg tttttatcaa tgtagtcggg ggagtaaaag
    23161 tgactgaaac agcgagtgat ttggctttgt tggctgcagt gatctcaagt ttacgtaatc
    23221 gtatatttga tagtgaaacc atcatattcg gtgaagtggg attagccggg gaaatcaggc
    23281 cagtgcaaag tggtcaagaa cgcattaagg aggctataaa acatggattc aagcgtgcta
    23341 tagtcccttt tgccaacgct cccaaacagc aactcgattc catgacggtc gagccagtta
    23401 aacatttgcg agatgtattg gagaaaatgt gagttatcaa aaaccaatca caccggaata
    23461 taaaatgaat gaagatacta tccgtgattt taatcgtgat ggtgcggtat gtcttcgcca
    23521 atttttaaat gccgaagaaa ttgctattct aaaagaagga atagaattta atttatcctc
    23581 cttaagtccc cgtgcaaaaa tagccagcag acacgatgat cctggttatt ttgttgagga
    23641 tttttgtact tggcaaaaca atccttttta tcaacgaatt atctttggta cgaatttggg
    23701 agtagctgcc gctcaaataa tgcaaagcca atcagtccgg ttgtaccatg atcatttgtt
    23761 ggtaaaggaa ccaggcacac gacaggttac accctggcat caggatcagc cgtattataa
    23821 tattgagggg aagcagaact gcagtttctg gattccggtt gatcctgtca gtcgttcttc
    23881 aactctggaa tttatcgctg gttctcattt gggtccttgg ttaatgccaa gaacattttt
    23941 agataatcag gcaaaatggt ttcctgaagg aagtttgcag gagttgcccc ctattgaaga
    24001 ggcacgcgca aggtatccaa ttattggatg ggaaattgag ccaggcgatg ttgtatgttt
    24061 tcatatgctg actttacaca tggcaagagg agttgaggga aggcatagaa gaagagtatt
    24121 ttctgtgcgt tttttaggtg atgatattac gcatgccccc agagcatggc ctacttcacc
    24181 tgattttcct ggtttgacgt ccgaaatacc ggcaggagca agcatggatc atccattatt
    24241 tccatgtttg ccactcaaaa tatagtaaaa cgatatattt tatttccctt caaaaaagag
    24301 ctcatttttt caaggatgag atttttaaga ttcctattca caacggatgt tattccaata
    24361 agcgtacaat caaaagggta tattttttat cattgactac caaaattcgt tattgttttt
    24421 cattaagtat tgtattgtat ttttgaagtg tctttatgta tagctgatcg agaggcactg
    24481 ttcatggtac atcggtttgc tctattaaac ttatgtttaa ccgtttgagg actaatatta
    24541 caaggagtaa ttatggcgtt acctacggag ttaaaaagca agttcattca aattctgaat
    24601 caaaacaaaa tttctcattc tgttggttgg tctaactaca tggagagtgt taataatcca
    24661 gccgcgattg ttgtggaagt taaaaatgaa aaagaggttc agacagtgat gaaggcgatt
    24721 aaggaattga attctgacag ggaacccaaa gataaagtga ctgtgagagc tactgcggga
    24781 tggactgata aaccgggtaa aagctgttgc ttttttccat ggagcgaagc ccaggagagt
    24841 cagtacaatg aagggttttc tttttcccaa gtcgtaggtg gcaaagagtc tcctgatacc
    24901 gcaggtaccg atgtcattat tcggtttgca aaaaaatttc accatgcaaa agtgattggt
    24961 tcattaaaaa atccacctgt ctttaatccg gacaatccca ttcatagact cccagtgact
    25021 ttggtggaag tgagcgctgg gatgcaggtg gctgattttg ccgatttttt gcgagaacat
    25081 cagttatcct tgtcaactgt tagcatgatt tcctgggtga cccctgttgg cttggctgga
    25141 acagcgggac atggtaccgg gcgtgatgaa ccagctttca gtggcctaat tgaatcggtt
    25201 acagtttgtg atatggatgg caacctgcgt gaaattaatt ctgatcatcc tgatttcaaa
    25261 gatttgacag gtggtcatag cgggttgttg ggtattgttg ttagcatgaa attacgagcg
    25321 gttaaggcat ttaatttatg tgaaacaata gagttatttc ccaataccag ggaaatgaca
    25381 ggcaggttaa gtgatatatt aaagaataat caatacatca gtattatggg aatgccaagt
    25441 tacggctgtc ctgaatcgga taaactggtt tccaaatggc aaattcgtca atggaattat
    25501 actaccgaca aaccagggaa aaaggaaaag gcaccttatg atccggacgt tcgctctttt
    25561 gctcaagagt tacaggttcg cattggtgcc tcagtgatgg atttcttact ggactcagga
    25621 ttaaagcatt tattaccttc ttttatgtta ctgtcagcag cagtagtcac gggtactcgt
    25681 ggtacgtcac caaaggtaga ttatgaaaat catatcacac accctcaggt ggcttttcca
    25741 aaggagatga gggatgtgag ttatttgatt ccagtcaaag atgacgaagc agggcaaaaa
    25801 ttggaactta ttttacaaaa aatggaagcc atgttgaacg aagcgggtag taaaggggaa
    25861 tttcctgtta cttatgcgat ttatgttcgt tatcttaagg ggactaacgg aggattatca
    25921 accagtttta cttcatcaga tgatgagcgt attttagcga tagacgtagt cactcatcct
    25981 gaagcgcctg ggattacaaa ttttgagaag ggattcatgt cttttttaaa agagatgaat
    26041 attattccca gaaatcattt gggaaaaaat ttccccgcgg gcgtagtgca ttatgatcag
    26101 tttttggggg cggagagggt gtcaaagtat aagcaggcac tggagagatt ctattgcagt
    26161 ccaggaaaga acgatggtgc tgagcgcctg agcatgtctc ctttttatac cccttatttg
    26221 caaggcatgt tatctgcaag tgcacctacc aagcaattag ttggtgaaaa taaatacgag
    26281 caacgcatag agaggttgca tgaggagcat tctgatctcg aatgtgctga gtttttgagt
    26341 aaattatatg ccgaggtgtc caggatgagt acgtcttcag aagaggctct tactgcaaaa
    26401 aaagggtttt tagaagcatg taaggcggaa ttggagcaca ggaacagtcg attagaagcc
    26461 cctgtacctc tattgagtta ggcaatcagg aaaataagta caagacggag cctagcaaat
    26521 tcaggagtct ctactaaact taaaaatata ataatatttt gagttaacct atggatatta
    26581 ctcctttttt taagctcatg gtcgataaag gtgcctcaga tttgtttttc agtgtcgggg
    26641 caccgccaaa tattaaaatc gagggcatca catcacctat tggcctggtt cctatcaaat
    26701 cgaatcaaat ggcagaaatt gttttatcta ttatgaatga cgcacagcaa aaagaatttg
    26761 agtctaaaat ggaactcaat atggcttttt cattagctgg agtagggcgt ttccgaatca
    26821 atttattcag gcaacgtggc gaagtggcta tggtggtgcg ttacatcaag ggcgaaatac
    26881 cttctataga agatttaaat ctccctcaaa ttttaaagtc tctggtcttg gagttacgag
    26941 gtttagtatt ggttgttggg tccacgggat cgggtaaatc aacgactctg gccgcgatga
    27001 tcaaccaccg taatgaaaat cacagagggc atattttaac aatagaggat cctattgaat
    27061 atatacatca acataaaaaa tcaatagttg atcaacgaga gataggcatt gatactctaa
    27121 gctatgacaa tgcattgaaa aacgcaatga gagaagcacc tgatgtgata ttaattggtg
    27181 aaattcgtga tcttaactca atgaaacatg cgatagctta tgctgaaaca ggacatttgt
    27241 gcatttctac tttgcatgcc aataacgcca atcaggctct ggatagagtg attaattttt
    27301 tccccgatga ggcaagacaa cagatattat tagatttatc tcttaattta aaagcaatca
    27361 tttcattacg tttgatacca ggagttgata atcaacgtgt cgcagcggtg gagatcttga
    27421 ttaactctcc ttacatttcc gatttgattg aaaaagggaa gatagacgat attaaagaag
    27481 tcatggcaag aagcacggaa caaggcatgc agactttcga tcaggcttta tttaatttat
    27541 ataaagaagg aaaaattacc aaagaaaatg cgattaagta tgctgattca aaaaacaatg
    27601 tgggtttaca aattcgattg ggcgaagaaa aaaattttaa tattcaagat gagaatttac
    27661 aaatacaacc cgatgatatt gataagtttt aattcattat gaattaattt tgaacattgt
    27721 tttgaatgaa acattgtgat aaaattatag tctaatttgt taatttttct tccagtccat
    27781 gcctagagtt tataatctta aagatattta tctgggtgcc ccttcttttt cagggcatga
    27841 agtttatctt gatgcggttt attatccttc tgatccgtcc gaaaaaaatt tcagggttat
    27901 ttataagaaa aacaagtttg gtaacgctaa cttatctcgc atggaagtgg cttttagtca
    27961 gttagcccga ttatttttag ataatggatt aacttccttt caaaaaatgg tagtgaatga
    28021 tgcaaataaa gtacagggat taatagttga gcatttaaat tatgtgattg aaaataaaga
    28081 aggattgaag cagccatttt atactttgaa tgcaccaagg aatggatgtg attatactga
    28141 aaagcgagtg accagctcga atgagatacc tttctatttc ctggataagt tgcctcaagg
    28201 tttttttaat caattactgg cggcagaaaa aaataataaa ttgaacatag attatgcctc
    28261 tttggcaagt atattggcca cttcgtacac tttggaagaa gacgatttgc ataaagggaa
    28321 ttttggcttt tatttggtga agaaaaaggg aaaaccgcga gttgtttttt ttaaaattga
    28381 tcatgattta atgtttgtag acagcatcat gtcatttaca acaagaagat tctgtcattt
    28441 gtttgatgga tgtgatgctt ttgatatcac agaagaagac ttgttaaaat ttcctaattt
    28501 aaaatattct gctaatggtt attggccaac caaaaccagc tttttttata aaccatggga
    28561 taataaagat tatcgtacct atgctgaaat tcaggctttt ggggatttat cccatgtgga
    28621 agagtttaac aaggcaaaat ggcggtcttt ttacaagcat attttaatct ctaaatctca
    28681 aatggaggct accttaaagg cctgctttga tgagaataac tcttcagaca gggcacatat
    28741 ttctctggta attcaagcga tgttagccag acaggcgcgt ttaaaggcga tgttattttc
    28801 tctaaaagat tttcgtgatt ttattctttc ccaaaatgga aaagagcgag atttattatg
    28861 tcatgaaatc ctgaataact tgccagaaga agagagaaaa tcttttgaaa atgaaattag
    28921 acaatcgtta gattataacc ataacctctg ttgttccggt ctttttgagg atggggatac
    28981 cccgctgcat atagcaatca agtcaggcga ttatcgttat gacgaaacga ttggcatgta
    29041 tgggcaattc atcaacacga aaaatagctc aggaaaaact cctttggata tcgctttaca
    29101 gatggcaggg cagtctaaag tttatcctgc ggatgtgcgt aaagattatc gttttatcat
    29161 gaaacattta ttggcgaatg gagctaatca gacaaaacag tttgaagaat tcgataaaat
    29221 tgaaaacatc agctcctatc aatttcatac tccttatcta aacaaagcca taaaggctaa
    29281 aacttatcat gaactgaaag aagtactcag ggacattggt gaagatcatc agtattgttt
    29341 gaaattcaaa aagatgttgg cagttgaatg catttctgaa tttataaagg ccaatcaaga
    29401 taatctcagt ttaagaggaa tactgttaaa attaaaaaag gaagtggatg gtaagggaac
    29461 aaaatcagaa aatgcggctt taatgtatat acgtcaatta agaagtcggc tatggattgt
    29521 acgtcaaatc agaggacttt atggctggtc tacaacgcaa ggcgaaattg attatatgat
    29581 tgataaagag ctggcacgtt tggatactaa agatttaaag agactttcat tatttgattc
    29641 cagagacagc agtactttgg ataatgtctt tttggatata tcactatcaa agaataaaat
    29701 ctaagttcat tctattgaga aaaataccaa tgtttagcaa tattattaag gcgtaatata
    29761 cgccttaata aatatatgca aactaccagg ttgattgtta aattaagcca taaatgatgg
    29821 ctgagataga aataatgccc atgacggtaa caaatacatt actgatttga ttgctgtatt
    29881 ttttcatggc tggcacttta gcaatggcat acattggcat gataaataat aagacagcta
    29941 tgatggggcc tcctaatgtt tcaatcattt ttaaaatatt aggattaatt gtagcaacaa
    30001 tccaacaagt cagaatcata aagatttcaa tgattctgtg cagggagttc ttattaattg
    30061 ttttgcctgt tgttgatttt aatgatttaa caataattcc acttaaacct tccttggcac
    30121 ctaaataatg tcccagaaaa gattttgaaa tggctatgaa tgcaataacc ggtgcaatat
    30181 aggccataat aggggtatta aaatgattcg ccagataaga caaaatggaa atattttgct
    30241 gctttgcttg caaaagatct tgaggtgaca ggctcaacac acagctaaat acaaaaaaca
    30301 tcacagagaa aaccatcatc aagtgactgt atttcataat taatgagctt ttctcgtcag
    30361 cttggctacc atattgctgc ttttgattca cagcaaaaga tgaaatgata ggagaatggt
    30421 taaaggagaa caccatcact ggaataatta accataaagt cattaatagt ccatgccctc
    30481 cattggcatg taaggaattg ctttggataa aaatagcgtt attccaattt ggaattaaat
    30541 aaagcgaaag cagaaataga atagtggcaa aaggatatac taataccgac atcgatttga
    30601 caataatttc ttgtcctaaa cgcacgatac ccattaaccc aagaattaat atgattgcta
    30661 aaagagctcg tggcggaggg gtaattccca tttgattaat caggaaactt tccgtggtat
    30721 ttgttattgc gacactgtac ataagcaaaa ttggataaat cgcaaaaaag tacaggatag
    30781 tcagcatctt acccgcaaat gagccgaagt gttcttctac aacatcggtg atgtcattgt
    30841 gtgaggacga gcctgaaagg acgaaacgac ataaagccct atgagaataa taagtcatgg
    30901 ggaaggccag aattgtcata atgattaaag gcaaaatgcc attaagacca gcatctatag
    30961 gaaggaaaag agttccggca ccaattgctg tgccatagag gcttaacatc caaatcgtat
    31021 cttgtttttt ccattgcgat agtttttggt tgactatcgg agatactata gctgcagatt
    31081 catttgatga catgaaaaac atcctcgttt taatactaac taataaaata gcgcctcgca
    31141 agttgatcaa tattaacact acaaagacaa tatttcaaca attgcgaact aaatcatatc
    31201 caactgaaat gaacaaagca agagattaat ttaaggtaag gagcgaggaa aatggccttg
    31261 ccttcttaac aattcatgaa gctcattccg attttattct tttaaattgt tgcaaatgaa
    31321 ttcgtattgt aataagaatg tttctcgaac caggtttaat taatggagta gttatgaaac
    31381 gaatcatttt gtttatgatc gcttggagtt tattgaattc agcctattct tctcactcac
    31441 ttccatccca tgccagcatt ctctttaccc ttgccaatac agagcgtagt aattcatgcc
    31501 cggctttgct gcataatgca aaggtcgttg ttgattatga ttataacttt gagcgaaata
    31561 tggggctatc tcacttaaga cagttgcaat cgaccaagtg gagcgaaaca ctacatccgc
    31621 tcggtttaag taattattat gcatttatgt cggatatgaa gcccaaagcg gttcagattg
    31681 aagggggtga ggtaaccatt tatcgaatta tttttcattt gtataaaaat ggcgatagca
    31741 ggatttatct tatgattaat caagatggcg aatgcgttat gagttctgat gttgtgaatg
    31801 ttaatttgta aagtaaatag tattaatttt aatgaaaagc tctcaaaaac ggggctagcc
    31861 ttggtcgaat gccaaattat ttattaacct tgattttgtt ttctgcccct tttttcatga
    31921 caatgatgct aatgcgccga ttttcaggat tgtagggatt ttttttatcg agaagaacag
    31981 tggaagaata acctgtgact gtcataactt tattttcgtc cattccagct tttatcaatg
    32041 cgcgtcttgc tgcattcgct ctttgtgcag ataactccca attggtgtat tccaattctt
    32101 caggattatg ataaggattg gcatcggtgt ggccttgaat agtgatttta ttaggaactg
    32161 aattgagtag ttttgcgatg ttagcgagta tgggttcaat ttccggattg attttatcag
    32221 agcctacatc aaacatgggt tttttctgat tatctatcag ttgaatgcgc aagccatcgg
    32281 acacaacatc catcaggagt tgttttttta gaccagcaag cgatggatct ttttccatac
    32341 ttaaattgat ctctgatttc agctcttcaa gtttttttat ttcattagct tgagattcaa
    32401 caatttcttt atcttttgct atgctttcgt tgattaaagg tttattggtg gcagaaacct
    32461 ggccatctgt gtcttcaata ttagggcctc ctcctttaat cgttgctttt ctattcccca
    32521 tgctttcccc gccaaacaat gccactttca ttggctgttt aaaatactcg gcaatacccg
    32581 ctttttgagc tttgtttagt gaagcaacta accacatgag tagaaagaag gccatcatgg
    32641 ccgttacaaa gtcagcaaag gcaattttcc aagagcctcc atgatgttta tgatcatttt
    32701 ttttaatttt tctgatgata ggggtatagt gagtggtttt ttcttttttt tcccgcttgt
    32761 cctttttttc cagatcgtcg ttgatcggat cgctgatgtc gtttatttcg ctcatggctg
    32821 taacctaact ggacgtagct tctgttgctt cagagttttg agttgttgtc tgagcggttt
    32881 tggcattttt tatttcatca tttaattgat taaatgaagg tctttgcgat gtaaataata
    32941 ctttacggcc aaattcagta gcaataatag gagggttatt gtgcaaactt gccaggatgg
    33001 taacttttat acattgcagc atcaattgat tttgattggc attttgctcc atagcagtag
    33061 cgataggtcc aacaaaacca tagccaatta aaatacctaa aaaggttccc actaacgcgt
    33121 gagcgattaa aacacctaat tcagagggag gtaagctgat ggattccatg gtatggacaa
    33181 cacctaatac tgctgcgacg ataccaaaag caggcatggc atcagccagt ttggtgatgg
    33241 cattggccgg gatcatttct tcttcatggt gagattcgat ctccatatcc atcaagcttt
    33301 ccaattgaaa aggctgcatg ttggaagtaa taatcaatct caggtaatca caaataaact
    33361 ctattaaatg atgattactc attaaattgg gataactggt aaaaattggg cttttatccg
    33421 gctcgtcaat gtcattttcg agcgacatta acccttgttg ccttgcttta ctgagtaaat
    33481 ggtatagaag gcttaacaaa tcactggagg cttgctttgc cgatttttgg ccgcgaaatg
    33541 tagcaggcaa agcttttaga acagatttta aaacggtaat attattacca acgataaagg
    33601 aacctatggc ggcccctccg attatcaata attcaacagg ctgaaatata gcggcgagat
    33661 ggccgcctgt caggctgaat ccaccaaata cacagagtag tattacaata taacccacta
    33721 taattaacat atccctatcc tatagtatct ggtgtccaat tagtcttgat attttttcat
    33781 agtgtctttg gcagacatgt catacagtgc tataccttca gtcattggga ctgattgatt
    33841 ccaaggcgat tttttgatcg catttttacc atcatcataa cctttttgaa tgcgttcatg
    33901 aatagatttt ttggagaatt cataatcttt tgaagacaat tccgtttcat cgtgctcata
    33961 taaaaatcga accagtgaaa tagcggaagg acgtcccttt ttaatacatt cccttatttc
    34021 aggttttgat ttttcctcag gagaaatatg gtttgataaa atgtgaattg tgtttcttaa
    34081 atcatgaatt tccttgtgaa ggttaattaa ttttataaac cgactggaaa actcaatgtc
    34141 ttttttacgc tttaatacct catccattga ggtgggaacc atcccaaaag aatcaaacaa
    34201 atgaatcata aaacaaagca gttgcttttg attgttatga ttcaggatat aattgactgg
    34261 tgaattactt gatataccgc catcccagta atatttacca tcgactttaa ctgccgggaa
    34321 accgggagga agcgcaccgc ttgccataat atgttcaggt cctatttttt gtttatagga
    34381 atcaaaataa gtcatcattc cactacttac ttcaacggct cctacactga ttcgggttaa
    34441 tttattgtta ttcagacgat caaaatcaat aaatttttcc aaactgttat gcaaaggaga
    34501 agtatcatag taacttaata aatcagggct gttatgccaa ccaaaatcag gggaagggaa
    34561 acgaggtaca aaaaaacctg gctgtcccaa gagtaaagcg gaatgagcag caattaaatg
    34621 ttgaattttc cgacttatct tatcatcgag gagagttgat tcattgactg cggagggagt
    34681 agctatagat tcccaaaatt gatacatttt ttctatccgc tctttgtcag agttacctgc
    34741 agcaatggcc gcattaattg ctccaataga ggttcccacg taccaatgag ggtggtatcc
    34801 cacttcattt aaagcatgca atactccagc ttgataggca cccaaagccc ctcctccctg
    34861 aaagctgcat gcaatatggt gatactcaat ctgtcttgca gattttttag ttcgagtcat
    34921 ggttgtttac tccatgaacc aaccatgact gactaccaat gattgtcctg taagcgcatt
    34981 cgattcaaat gatgcaaaaa ataaagcggt ttgtgctaca tcgtctgtgg tggtgaattc
    35041 tccgtcaact gtttctttta gcatcacttt cttaatgaca tcttcttcac tgatgcctaa
    35101 ttcttttgct tgctctggaa tttgtttgtc aacgagagga gtacggacaa accccgggca
    35161 aatgacattg gctcttacct tatgaattcc accttccttg gcaactactt tgcataaacc
    35221 caatagacca tgtttggcag taacataagg tgctttgagt acagaggctt ctttggaatg
    35281 gaccgagccc atataaataa tactgccgcc attgcctgag gcatacatgt atttcaagca
    35341 agctttagta gttaaaaagg caccatccaa atgaatggcg agcattcttt tccagtctga
    35401 aaaggctaat ttatctactg attcgatgat ttgaataccg gcattgctga ctaaaacatc
    35461 aactcctcca aagtgatcag ctactttatc cacgccctga ttgacttgtg cttcatcagt
    35521 tacattcatg gcaacggcca ttgcttgtcc gcctgatgat ttgatttcct ctgctacatg
    35581 gcttgcctga tcaagattca gatcagcgat tgctaccttt gccccttctt tagcataaac
    35641 aagcgcaatt tctttaccaa ttccgcttgc tgctcctgtg acaatagcaa ctttattctt
    35701 cagtttcatc tttactatcc ttgtcattgt tttccatgaa ttgattatag caatggattt
    35761 ttaataatgc cttattattt gctaacaaaa atctttgatg aattgtttaa attttttaga
    35821 gagttgtctt taagggaggt aattgcttct tttctgtatc atttcgccaa tagttttggt
    35881 tttacccaaa tcaagaacta tcccggccct ttctctatct agaaaagacc aggtt
//
LOCUS       DASLOE010000017        35026 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.017, whole genome shotgun sequence.
ACCESSION   DASLOE010000017 DASLOE010000000
VERSION     DASLOE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 35026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35026
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.017"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(113..829)
                     /locus_tag="U6309_000645"
     CDS             complement(113..829)
                     /locus_tag="U6309_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEN5504100.1"
                     /translation="MLVTKEVEYEDGDTVCKGFIAYHSSVSKPLPCVMVAHDWGGRGE
                     GVCNKAIQLAGMGYIGFAIDMYGNAKLGKDKVEKRALMTPFREDRGKLITRINAAFSK
                     VVQLPEVDSDKVAAIGYCFGGLCVLDLIRSGAPLRGVVSFHGVLLPLEGESNKPLHAK
                     VLILHGYDDPLVPIEQVNQFAIEMTERKVDWQVHVYGQTAHSFTDPNANDDEMGLHYN
                     KLADQRSWQSTQLFLQDLFA"
     gene            complement(960..4064)
                     /gene="dotA"
                     /locus_tag="U6309_000646"
     CDS             complement(960..4064)
                     /gene="dotA"
                     /locus_tag="U6309_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein DotA"
                     /protein_id="HEN5504101.1"
                     /translation="MNKLAITVLLCFFPALALAESGSLSFAPPASDLSVVFLGNLFGV
                     VDGVLHGTGSQIMGNMFGVFNSAVLALGGIIIMYTLMVSTMNTAHEGQMLGQKWSSIW
                     IPLRSTFGLALLIPKASGYCMMQVFFMWVIVQGVGAADKIWEAALSYLNRGGVIIQAQ
                     SDPTKSLTDKGGKSTGIAKGAFTILSGQVCMLGLQKQLQTQRNLYLSQSNSPPCGGNP
                     TPEMNTFCRTAIPDFISTVNFVKKQSDDKSKGPFSLDMPNFDKSSPFYFLNGICGTVT
                     WNSISDLKADMGKNIKLTSSQLETAQLSRAIAIQQMYVTLSTVAQVMVNNDPAFSSTS
                     STGNSKNDFSAIAKQQFGVPYKASGQVCTEYQQVCQTWGSIPSSTGSTTGVLFNGTEF
                     LGAINDYNGIMTPTINLIKQSKSAEYDDKSRDFIQEANTKGWIMAGSYFFDLVKLNGS
                     AVKDESDFDSDTGLDNSNFDPAQLTKPFGKTCQGTYALLCNWFQNKSDKLVQIQSLIN
                     GAPALSQDGVKQPDLSPDPKRAYVEGPLSSTVYGFINNSMMVQLPGQPGIKPLTFANL
                     INFKVDTSLYYMKHQDFDCGRVKILFFSFCLGRMMGDLFYNYVFRYVYNFFLAIFGEM
                     INSIVMAFLMIPLQGMKDIFIVGVQTLAQPGVNPIVALANMGTMYINFSGTLWLTLLN
                     MAVVSSLIPLFGIFIFALIMMAMPLLMAWIGTMVSIGFVTAYYIPVLPYMIFTFGSFA
                     WLIAVIEAMVAAPIVALGVTHPEGNEAFGKGEFAIMILVNVFLRPSLMIIGYITAIAL
                     SYVGVWILNAGFDHAISYIQSDQGIETWKKGDFADKNANSAYCQMYPADCKMIQAGQT
                     VANWTGSKIDTSQWDDFGSKLSGDYQSTALQGGYSGWAGVYAFFFSILIYTTMYLIIV
                     QKSFTLIAHLPDKVLRWIGGSPESFGQETTQWGEEAKGKVEKAGEATYGAEKAIGDKL
                     GAKGQEMLGKFGPQAKQISENAGDVSGKGKNSGSQTGPSSKPDTGQLGGSDSGTPTST
                     PPPE"
     gene            complement(4061..4516)
                     /gene="icmV"
                     /locus_tag="U6309_000647"
     CDS             complement(4061..4516)
                     /gene="icmV"
                     /locus_tag="U6309_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmV"
                     /protein_id="HEN5504102.1"
                     /translation="MKKKSGSRIVRVITRIINVRKWVDWDRMKSFTLYLVNGVKRLFV
                     PQEPTHVESFDEAVKKLKLNETDLIIKQKALFRLSVIMVIAAFLLLIYTGYQLFYGSW
                     KATIISLVVVMIALVLAFRYHFWYYQIKQRKLGCTVKEWYRQGLLGEKE"
     gene            4629..5084
                     /gene="icmW"
                     /locus_tag="U6309_000648"
     CDS             4629..5084
                     /gene="icmW"
                     /locus_tag="U6309_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmW"
                     /protein_id="HEN5504103.1"
                     /translation="MPDLSHEASAKYWFEYLDPMIYRVITFMESVENWTLDGNPELEE
                     AMKQLGQELDDIEKIDLGLLAEEDKFIRIVGNIKSGRGLRLLQAIDTVHPGSASRVLI
                     HAEETSLSSNDPAGFFLKRNIVFERLRLLSRVFCQYRLKLVLRALEGDE"
     gene            5084..6490
                     /gene="icmX"
                     /locus_tag="U6309_000649"
     CDS             5084..6490
                     /gene="icmX"
                     /locus_tag="U6309_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmX"
                     /protein_id="HEN5504104.1"
                     /translation="MKVLPKLALANLFCFTTLSVFAQGQGYDPSYQQTSTNTSNLVTY
                     LTNLGKYLGYDITQSNKTPNPPYSQLFNSNVAQLIQSYAYNTFLGAIPVDAMSQSLMN
                     FVTNNVQGNALINNLANSTFKYQNFSSPSSGADGKITANGIIDQSTTTAQAPTPTGIF
                     SAPQAPTAGTYLNDPVSQAVFNILGTPDYSFCMDNDQKNWKSDCKLNYQNLVMQNVIG
                     TLPPALSQAGPVFYSYEYNKPIISQLNSNSLIAPLLLDTSSSQSGQNSGLTAKSQAQQ
                     ALNFIRYASAQVTPPSLPKLSAYSELWNQATAKPNSEGYNEVQQKQAAATLSGYFNNL
                     RVYAAQTSVGVSNLYYILSKRLPQNMSGDQNSSNITSQALNEFNMATRRLFDPTASNT
                     PGQPNQQWIKQINDASPATVQKEIAVLLAEINYQMYLDRQIQERILLTNSIMLLQNLK
                     AAQPTADFSNQSSPSEQQ"
     gene            complement(6491..8134)
                     /locus_tag="U6309_000650"
     CDS             complement(6491..8134)
                     /locus_tag="U6309_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein LphB"
                     /protein_id="HEN5504105.1"
                     /translation="MSSLLSRRKTILVLLIAIYCAYDLMVALYTAWDFTTDDAYISWY
                     YARQLVSGKGLFWHATLPPVEGYSNFLWLMIAALVIKLQLPLVSSMKVISCCSLGLAL
                     LFLYRLARLFCSPLLSILPVFLFSHYTGVTWWAVSGLETLFYCALSLALIWQCTLAFG
                     YQTVDDRPWPNRFSLYSTTHWIITNMLLLLLSLTRFEGIVWIIPLAVFIGCQYLSAQR
                     ELGFKNHKRLFLWGSTTFICFVLPYLVYFIWRLIYFSHWIPNSYRCKALAYGQFLAVD
                     FDYFLFITPLLVLSVPYLLSANKDCRHVLLWLPSVLYTLLLWKANPVVTYGLRLFLSP
                     FALFSILPVLGLNQLIQYFKFFRWDPEYLAAVLMLFITWFFVPGNDLNHLSKSVAEYN
                     ERTQNRMTIADLLNRKAKRGDSVLLDDCGIIPFFSREDIRFIDAQCLNNSDLTQKPFK
                     NDLSLYADYLNSKVKPDWVITNYYPMESRGDYLTDLLYEKHFFKNYQLVSTLNSGFYL
                     NKNQPQGKKMIDFVYQIFKRVNKPGYNGSLELESQEPKW"
     gene            8500..11043
                     /locus_tag="U6309_000651"
     CDS             8500..11043
                     /locus_tag="U6309_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN5504106.1"
                     /translation="MSKPYYFYVSGMNCTSCSNAIESFLRNSNLIKINTFHADMSTPD
                     PKKIVISLSEEKENEHESNWKTIKKLIDDTGFTCENYEFPQVRDQKPALPLEKTQTTV
                     QQESSQLSALFSSAKKIITSHWLLGGLGCITGVSLMILTMALGGLPLAVMIPLASIST
                     LLTIALGAQSYYEAWKKLVQSRTLTMDTLFSISTISVLVVSLASFFVPWLPMMFEAGL
                     LIYGFRHIGIAIEDTIKDKISSAKFQDRAPQKVRLYRHPDFKKTRIDEIQPNDIIQVK
                     PGELIPLDSVCEQDTLIYPTIITGAVIPKQFRSGEKILAGMRLANDAKPLSLRVLKTA
                     KESYLARLDESIAQSVLEKAPIEVKTGQLLSYFIPSIIALSILSGIIVGLIATPALAI
                     QCAISVLVSACPCTLGLIIPLAVKTGIHKGAEHGVQFKNSRVLQLAEQIDVVIFDLNG
                     TLTTGIPVVKEYAVLNDADLSSEQFLSICNVLEEQSKHPFGKAIQSFTQGINKQKFNI
                     ANLDHSNHSGISAEINGIQYTIGSKTLMQEIGISTSSLENQMNLVAGDSLVFLARNKQ
                     LLGYLVITDPLRKDAKQTVDALIKMGKEVHLCTGADEKTAYRYAQALGIKKVHANCVA
                     TTINTEDRSKPVYIKSLQNQGLKVAMIGDAANDANALAASDFGIAVLSQDSDEITQQH
                     ASAIIQSGTILAIANAFAISKQTVNNINQNLLISLGYNLTTVLIAGGLLVTLGFVLNP
                     AVGVALMVTQACFVLLNVYRFKEQPLQHLQDKVQEKPEPVPSSYEAMKKHSPNFHTAP
                     EINHKSVVQSGKQPTNKNKSPSFWSDYFPELFEEEKDTGTEHLMNNKIV"
     gene            11294..11824
                     /locus_tag="U6309_000652"
     CDS             11294..11824
                     /locus_tag="U6309_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2692 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504107.1"
                     /translation="MPTTFEDKSSKVPSLKSLAANAVQKANANLFFRLHSLETPPEIK
                     KGFIDKELEALTHELTEDYQTKVEARNEKIEECSSNLSSNECFVKCSAFTLTTLMAGV
                     HVGIYYILKAAAVDPSTQIAYISSIPATICFSMCVGVCLNRQITKCLSSCFTPSVPDK
                     ITVDLDELGRQSHVSP"
     gene            complement(11896..12696)
                     /locus_tag="U6309_000653"
     CDS             complement(11896..12696)
                     /locus_tag="U6309_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5504108.1"
                     /translation="MDNESLLAILNNWSATNYAKGNYILREISSKFLAKISIHSNDYL
                     LDAGCGDGSFTQMLANLVPDGYVLGLDRSKTMIDYANKHCRSINVRFDIGDIQEPIIY
                     GPFDNILSFWCLHWTDLEKSLSNLYHVLKPGGKICAIFSSGKQSTLFEVLNSLQFQNL
                     YPDVAKNVVQSKMMRENYSVQCRRALDNLPFKSISFIVKHCQVELPDLGLFENFIHGA
                     PLFKDLPGKMYQDIVNSMVQFFDEYCKKKYGGKWVYETTPYFLTITRE"
     gene            complement(12820..13677)
                     /gene="legD1"
                     /locus_tag="U6309_000654"
     CDS             complement(12820..13677)
                     /gene="legD1"
                     /locus_tag="U6309_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LegD1"
                     /protein_id="HEN5504109.1"
                     /translation="MILIFEEHRMQLSSEQCDFFFTNGYLVINNFFSESVCDLLKQRI
                     ETLLENNQAEIPKTIFSTQTNEHAKKQYFLDSGDKIHYFFEPGAFDEAGDCLRPFNQS
                     INKIGHALHELDPVFRQYSRDDRIKKIAHQLGLKTLGLVQSMYIFKQPGIGAEVLCHQ
                     DSTYIFGEDSDALGFWFAIEDATLENGCLEVIPSPCITPLKQRMFRRENEIYFENFDS
                     SPWPEENSVPLPVKKGALIILHGRVPHKSQANFSNQSRHAYTLHLVDISLPYPEKNWL
                     QWPNGIPCL"
     gene            complement(13766..14752)
                     /locus_tag="U6309_000655"
     CDS             complement(13766..14752)
                     /locus_tag="U6309_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEN5504110.1"
                     /translation="MLVPLIRTLQNKLPNAQLTWIISKPAFYLMEGMEGVEFIVIDKP
                     KTLADYWRFRKQMLPRQFDVLLAPQASFRTNWLIPFIKAKRKVGYDSHRANDGHRLFI
                     KESITPGLEHTLEGFLKFAEPLGVTEKIIHWDLPITKADYEWAQNHLPEQERPILVVN
                     PAASKPERSWLAERYIEVLKQAQVKWDVKVVLTGGPGDHDKSLAEEIAKEIPVVNLVG
                     KTKPKQLLAVISKAKAVLCPDTGPSHMAAAVGTPVVALHAVTNPSISGPYTFQHLVVN
                     RYSQAIQTVLGVSPEKSVWGTQVHGYEAMKLITVDDVMEKLTEIFSGLMNNT"
     gene            complement(14796..14990)
                     /locus_tag="U6309_000656"
     CDS             complement(14796..14990)
                     /locus_tag="U6309_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEN5504111.1"
                     /translation="MRKTTKEPASAKKNYVVHRHQLPLSCPTDEMELWNAHPKVYLPI
                     EKTGVEVCPYCGSRFVLQND"
     gene            complement(15013..15954)
                     /gene="miaA"
                     /locus_tag="U6309_000657"
     CDS             complement(15013..15954)
                     /gene="miaA"
                     /locus_tag="U6309_000657"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027225287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEN5504112.1"
                     /translation="MNKLVFCLMGPTASGKTGLACDLVTHFPFEIISVDSAMIYRDMN
                     IGTAKPSIHELQRAPHYLIDIKDPVESYSAAQFCTDALSLCAEIIKRGNIPLLVGGTM
                     MYFNALQKGLATLPEADEAVRKRLEEEALSQGWDFLYQKLSQLDPVTAARIHAHDTQR
                     IQRALEVYYLTGSTLSTYLTGPHVQPDYYFVNLALFPEQRSWLHERIAQRFDAMLSEG
                     FIEEVQQLQAKWPIQINLPSMRCVGYRQILEYLAGHYDYETMREKGIAATRQLAKRQL
                     TWLRHWEGALFYDSQNVGFNTDIIAKIREILDNTVSN"
     gene            complement(15947..17680)
                     /gene="mutL"
                     /locus_tag="U6309_000658"
     CDS             complement(15947..17680)
                     /gene="mutL"
                     /locus_tag="U6309_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEN5504113.1"
                     /translation="MVLRIHQLSPAIANQIAAGEVIERPASVVKELLENSLDAGASVI
                     GVDVNYGGLLQITVSDNGSGIFGDDLPLAVAAHATSKIRTLDDLYSIDSMGFRGEALA
                     SIASVARVTIISKPEEQDNAMMLRVEGESRTLSPCARNIGTTIDVSDLFYNAPVRKRF
                     LKNEKLEFQAIEMVVKRFALSAPRIALTLKHNKKLIFSLPPALSEQAKAIRMGRILGN
                     TFMREAIFLDVEHSGMRLYGWISNHRLQRSQNDRQWIYVNQRMVKDKLLQHAVKQAYD
                     GLLHAGRFPICLLYFTLPASEVDVNVHPTKHEVRFQQPRLVHDFFTSQLTKALQSTLD
                     HSDTREYAYAASSCNEQQNTVSEPSHICNDRESHEEGARALSPSWSANLLSSSITNKR
                     DKKTSSVYHPETEFQWVILNERFILVFIKQQPYLADIVTLFQEWIEKQLMQKALPLES
                     RPLLISIRYPLPNQWMHKQEQLKHELARVGIQIEYPNADDLIIRSIPLNAPYLDVRCF
                     FNVLCELDIFHTEQLIALISRSQIFDPKQLTVEERIEMNQLLLELSLKEEKHNICKAL
                     TIDDCRMLLHE"
     gene            complement(17671..19101)
                     /locus_tag="U6309_000659"
     CDS             complement(17671..19101)
                     /locus_tag="U6309_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN5504114.1"
                     /translation="MMMRAWVLSLLLYLPSSFAVFSAQLKNITVSQQPTMTSLYLSIQ
                     GSFTHKLFTLSQPNRVVLDLSDTQLAVNISQLGLINSLIQQVRSGSPSKNTLRLVMDV
                     KQPVKVRSSSWKPKGEFNGIRIDLVNDTPLVINNRRQLSPPVKTIKAPVQQIQSKISS
                     QPSEQRITVHKTPVQVPHNPSKKPLRDVVVVLDAGHGGKDPGARGPRRSNEKDVVLAI
                     TLKLKRLIDRQPGMRAVLTRSGDYYVGLRQRLDIARKYNGDVFISIHADAFNNPHSNG
                     ASVFALSQRGATSEAARWLAEKENYSELGGVNLGELDDSNGVVRSVLIDLSQTATINA
                     GLEMGGKVLGQLDNFTNLHNNKVEQARFVVLKSPDIPSILVETGFISNPREERNLTNP
                     AYQERLSQAIFQGLKNYFWDHPPHGTRIEAMISPNFHIVKKGETLPAIAARYRVSVSA
                     LQSINRLQARARLMPGQKLVIPGTWS"
     gene            complement(19101..19583)
                     /gene="tsaE"
                     /locus_tag="U6309_000660"
     CDS             complement(19101..19583)
                     /gene="tsaE"
                     /locus_tag="U6309_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEN5504115.1"
                     /translation="MTIEDNDNFITLDLVSEQESKRFAEKLAFCISAPLVITLSGDIG
                     AGKTTIVRAMLKSLGVISAIKSPTFSLVESYNCGQFHIHHFDLYRIQQEEELEYLGFR
                     DYFSNQSVCCIEWPEHGGKTLPPVDIQFNLIIKGTGRLIQIAALSVAGKRILACLAGE
                     "
     gene            complement(19710..21191)
                     /locus_tag="U6309_000661"
     CDS             complement(19710..21191)
                     /locus_tag="U6309_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HEN5504116.1"
                     /translation="MNKLENALYRCDQIKACEQQAATLYQKDENALMALAGAEAFSFL
                     QKLYPHVRHIAVFCGAGNNAGDGYVLARLAHEQGFSVTVYQCKSIEDLPSAARNAARL
                     ALTSGVHCQSADEPLDGDAELIVDALLGIGLQGAVHGAIANAIHQINASGLPVLSIDI
                     PSGLNADTGLVENICVNATATITFIGLKTGMYTLDGPDYCGLIHCAHLNLEACTTKLT
                     RFASLLRDSTLPLPLAARKKNSHKGDYGHVLVIGGGPGMPGAVSLAAKAALKTGTGVV
                     TVATWPDYAKSALPLIPEAMIWGIKTAGELMPLLEKATVCVIGPGLGDSDWAKTLFLT
                     AITTQLPMIIDASALRLLSQHPQMDDNWILTPHPGEAASLLSCTARDVQMDRYHAASA
                     IQKQYGGVVVLKGAGTVIQTAERNIFVCPQGNPGMSTAGMGDILSGVIAAFCAQGFTL
                     SDAAKLGVWVHAVAGDKVAEKSGEVGVLASDLLDEIPSILNCI"
     gene            complement(21319..21714)
                     /locus_tag="U6309_000662"
     CDS             complement(21319..21714)
                     /locus_tag="U6309_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEN5504117.1"
                     /translation="MAMTSNKPYLIRAFYDWIVDNDLTPYILVDASNPNVQVPREHVQ
                     HDRIVLNISPSATRGLLLENDRIVFTARFSGQTEQIFVAPNAVLEIYAKENGRGIAFS
                     VDEDDEPPPKTSSSSESATKNKPSLKLVK"
     gene            complement(21717..22337)
                     /gene="sspA"
                     /locus_tag="U6309_000663"
     CDS             complement(21717..22337)
                     /gene="sspA"
                     /locus_tag="U6309_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HEN5504118.1"
                     /translation="MAIVAKRTIMSLFSDVDDVYSHQVRIVLAEKGVNVEILAVKQDE
                     PGADLLSINPYGTVPTLIDRELVLYEARIIMEYLDERFPHPPLLPVYPVARAEARKMM
                     HRIEQDWYYLMNAILREESVEQARANLLESLTSLDPVFADKPYFLSDEFSLLDCALAP
                     LLWRLPKLGLEIAPNFKGIISYMQRVFKRDSFQTSLTDAERQIRAA"
     gene            complement(22588..23328)
                     /locus_tag="U6309_000664"
     CDS             complement(22588..23328)
                     /locus_tag="U6309_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEN5504119.1"
                     /translation="MMKIKVLLVAALAFLMSNAAFTENLTMDYVPVDINDKESLQRGA
                     KMFMNYCSGCHSLKYMRYNRMAQDLGLTTFDGQIAEDLLKNNLIFTQANIYDPIQIAM
                     SPEDAKQWFGMVPPDLSLSARDRGPVWIYNYLKSFYSDPSRPFGANNLLMPDVAMPNV
                     LEPLIGKVILVKDKNSHANSLLVVRRGEMFEGQFDSALRDLVNFLVYVGEPAKLIRYK
                     LGVFVLLFFCIFLIAAYSLKKAYWKQLK"
     gene            complement(23325..24539)
                     /locus_tag="U6309_000665"
     CDS             complement(23325..24539)
                     /locus_tag="U6309_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEN5504120.1"
                     /translation="MNGLVNWLDERFPLVSTWKAHFSNYYAPKNFNFLYFFGSLALIV
                     LVNQIITGLWLTMFYTPNAEQAFSSVEFIMRDINFGWLLRYMHSTGASAFFIVIYLHM
                     FRGLLYGSYQKPRELVWLLGMFLYLLLMAEAFFGYLLPWGQMSYWGAEVITSLFGAIP
                     YIGKGLAVWLRGDYSVANPTLQRFFALHVIGIPILMIILVFLHIVALHKVGSNNPEGI
                     DIKKALDKDGKPLDGIPFHPYYTVKDFVGIIVFSILFFAVVFFAPEMGGYFLEHANFV
                     PANPMVTPDHIAPVWYLAPFYAILRAIPDKLIGVVCMAAAILLLFFLPWLDKSNVRSV
                     RYKGMYSRISITLFAFSFLLLGYLGTTPVTPVRLLLARIAAVIYFSYFILMPIYSRFE
                     KTRPVPTRIGEK"
     gene            complement(24550..25167)
                     /gene="petA"
                     /locus_tag="U6309_000666"
     CDS             complement(24550..25167)
                     /gene="petA"
                     /locus_tag="U6309_000666"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEN5504121.1"
                     /translation="MTDLNQDVSNHNQDEQLDEERRRFLLHTTCVLGGVGAACALTPF
                     ITSWLPSSKAQAAGAPVKVDVSRMEPGEQAIIEWRGKPVWIIKRSKDMLNQLQKEDES
                     QLRDPDSLVDQQPEYAQNKFRSINPEFLVLIGICTHLGCSPKYKPNLGELGPDWPGGF
                     FCPCHGSMFDLSGRVFKGVPAPINLEVPPYHFLDDHTIVIGEDAK"
     gene            complement(25341..25772)
                     /gene="rpsI"
                     /locus_tag="U6309_000667"
     CDS             complement(25341..25772)
                     /gene="rpsI"
                     /locus_tag="U6309_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN5504122.1"
                     /translation="MAEMKQYYGTGRRKSSIARVFLRPGKGDIKVNDRTLQEYFCRET
                     SCMIVMQPLETVDLLNKFDVYITVTGGGISGQAGAVRLGIARALVAYDEDGLAEGAEP
                     SPDSFRRKLRARGLLTRDSRRVERKKVGLHKARRATQYSKR"
     gene            complement(25778..26212)
                     /gene="rplM"
                     /locus_tag="U6309_000668"
     CDS             complement(25778..26212)
                     /gene="rplM"
                     /locus_tag="U6309_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEN5504123.1"
                     /translation="MKTFSAKGNEVKRDWFVVDASEKVLGRLATEIARRLRGKHKAEY
                     TPHVDTGDYIIVTNAEKVIVTGRKFKNKMYHHHTGFPGGIKSASFEKLQDKNPTKIIE
                     LAVKGMLPKNPLGREMYRKLKVYAGSEHPHTAQQPKQLEIEE"
     gene            complement(26552..26869)
                     /locus_tag="U6309_000669"
     CDS             complement(26552..26869)
                     /locus_tag="U6309_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEN5504124.1"
                     /translation="MLSHYTKHVFICTNQKAPGKQCCANSGGEEFFDFMKSKLLEFDL
                     HGPGKIRVSKSGCLGRCGSGPCIVIYPEGVWYTYSSFEDIEQIIKYHLIDGEIVAPLL
                     LDR"
     gene            complement(26871..27164)
                     /locus_tag="U6309_000670"
     CDS             complement(26871..27164)
                     /locus_tag="U6309_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEN5504125.1"
                     /translation="MNALSKAIMAETLCDELKLNKTVAKEMVENFFEELRHALENGQH
                     VKLSGFGNFTLRDKPQRPGRNPKTGEEIPVEARRVVTFKPGLKLKTKIEKIGK"
     gene            complement(27167..29548)
                     /gene="pheT"
                     /locus_tag="U6309_000671"
     CDS             complement(27167..29548)
                     /gene="pheT"
                     /locus_tag="U6309_000671"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEN5504126.1"
                     /translation="MKVSKLWLREWVNFSLTEQELAEQLTMAGLEVDAVNPVAGQFTH
                     VIVAEVLNTKPHPDADKLTLCEVNINKDKPLKIVCGAANVRPGLRVALAMIGAHLPGG
                     LQIKESKLRGELSQGMLCSATELGLAEHSEGIMELSDEAPIGMDLREYLALDDHVFDI
                     DLTPNRADCFSILGVAREVAVLNKLPLIEQPIVTVPPAIDDGLRVNLIHSEACPRYCG
                     RIIRNLNLEAKTPLWMAERLRRGSIRTLHPVVDVMNYVMLELGQPMHAFDLSKINGEI
                     NVRYSASGEQLELLDGQEVVLNDNVLVIADKEKPLAMAGIMGGANSAVQEQTQHVFLE
                     SAYFNPVTIAGVARKYGLFSDSSQRFERGVDPCLQSKALERATELILSISGGEPGPVI
                     ESFDKKFLPGTVSFLFDTTKVKKLTGLSIPLNEMKNLLEGLGVVITKETNHFFEVTIP
                     SHRVDLQQDADLVEEIIRLYGYDKLQAQPMQTSLQAGLISAKEKIATHVSSWFSAKGY
                     HETISYSFVDPELQEALYPQKEFMELLNPISSELSQMRAGMWPGLIASMIYNSHRQQT
                     AVKFFEIGVVFDLDGGQLKERSCIAGLLMGEQGNLNWSESARLFDFYDLKGDLQSLFA
                     SLKLDDVEFIQSSHHALHPGQSAQIVINGKHSGWIGVLHPRLSDAFDLDQDVVLFELN
                     LESLINPTIPLYKPISKYPQIRRDLSFLVDRQISAMQIERVIRNTVKEDWLKSFDVFD
                     VYMGKGIPEDKKSIAVAMTLQDDTRTLVDAEINLTISAIIKKLENEFSILLRE"
     gene            complement(29805..30821)
                     /gene="pheS"
                     /locus_tag="U6309_000672"
     CDS             complement(29805..30821)
                     /gene="pheS"
                     /locus_tag="U6309_000672"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEN5504127.1"
                     /translation="MLVLINTIQEQASEAIKQATDIVALEQIRVDFLGKKGKLTELLK
                     GLANLSAEEKPKVGQLVNQAKQGISALIETKMIELKEKQLLAKLAAEQIDVTLPGRNH
                     STGSLHPVTQVKHRINDYFSRLGFDIVEGPEIETEFYNFEALNIPGHHPARAMHDTFY
                     FGDGRLLRTHTSPVQIRTMEQRKPPFRLIAPGRVYRCDSDVTHTPMFHQVEGLLIDKQ
                     ATLAGLKGLLQDFFAYFFGRELALRFRPSYFPFTEPSAEVDIECTQCNGKGCRSCKFT
                     GWLEVLGCGMVHPNVLIAVNIDPNEYHGWAFGMGMDRLAMLYYGIDDLRMLFENDLTF
                     LRQF"
     gene            complement(30958..31317)
                     /gene="rplT"
                     /locus_tag="U6309_000673"
     CDS             complement(30958..31317)
                     /gene="rplT"
                     /locus_tag="U6309_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEN5504128.1"
                     /translation="MPRVKRGVTAKARHKKILDQAKGYYGARSRTYRVAKQAVIKAGQ
                     YAYRDRRQKKRQFRALWITRINAQARECGLSYSRLIDGLKKASIELDRKILADMAVHD
                     KVAFAAIAEQAKAALAG"
     gene            complement(31333..31533)
                     /gene="rpmI"
                     /locus_tag="U6309_000674"
     CDS             complement(31333..31533)
                     /gene="rpmI"
                     /locus_tag="U6309_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEN5504129.1"
                     /translation="MPKLKSHRGAAKRFRKTASGAIKRRGAYRNHILTKKSTKQKRHL
                     RVEAGTLKPCDARLAERMLHGS"
     gene            complement(31554..32090)
                     /gene="infC"
                     /locus_tag="U6309_000675"
     CDS             complement(31554..32090)
                     /gene="infC"
                     /locus_tag="U6309_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEN5504130.1"
                     /translation="MSAPTKRENDRARINEQITVPEVRLIDVDGNQAGIVSTREALRA
                     AEESGLDLVEISPTAKPPVCRIMDYGKFLFELSKKQAEARKKQKQIQVKELKFRPTTE
                     DGDYQVKLRNLIRFLNHGDKVKITLRFRGREMAHQELGMKILERLQADTADVAVVEQH
                     AKREGRQLLMVLSPKKTK"
     gene            complement(32115..34028)
                     /gene="thrS"
                     /locus_tag="U6309_000676"
     CDS             complement(32115..34028)
                     /gene="thrS"
                     /locus_tag="U6309_000676"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEN5504131.1"
                     /translation="MPNVKLPDGNVKHFEAPLTIYDVAHHISPGLAKAAIAGRVDGVL
                     VDTSYLIKEDCSLIIVTEKHEDSLEIIRHSTAHLLAQAVKALFPSAQVTIGPVIEDGF
                     YYDFAFERSFTPDDLSLIEAKMHELAKANLSITRRELPRNEAIQYFKGLGEEYKAKII
                     ADIPENEALSLYRQGDFEDLCRGPHVPSTGFLKAFKLTKVAGAYWRGDSNNEMLQRIY
                     GTAWADKKSLEEYLFRLEEAEKRDHRKLGKALDLFHFQDIAPGMVFWHPKGWTIYQEL
                     EHYMRNRLVDFGYQEIRTPQLVDRSLWEKSGHWANFRDEMFVTETENRHYAVKPMSCP
                     CHVQIYNHGLKSYRDLPLRLSEFGNCHRCEPSGALHGLMRVRNMVQDDAHIFCTEDQI
                     QSEVAMMLELVQSVYKDFGFTEIKYRLALRPEKRVGSDDVWDKAETALKLAMQGRNIE
                     WVDAPGEGAFYGPKIECSLSDCLGRIWQCGTIQVDFSMPARLEASYVAEDGSKQTPVM
                     LHRAILGSLERFMGILIEHYAGKLPLWLSPVQAVVLTISEKQNEYAEKVRKTLQKRGI
                     RANFDLRNEKIGFKIREHTLQKIPYLLVVGDKEVENCQVAVRTRDGIDLGVMTIDTIC
                     DTLTQEIIRKGSI"
     gene            complement(34156..34230)
                     /locus_tag="U6309_000677"
     tRNA            complement(34156..34230)
                     /locus_tag="U6309_000677"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34196..34198),aa:Val,seq:gac)
     gene            34543..34830
                     /locus_tag="U6309_000678"
     CDS             34543..34830
                     /locus_tag="U6309_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEN5504132.1"
                     /translation="MWFKIIHPHVEIAIYAKPNAKKTKLMAISDDRLHIALHAKPQEG
                     EANNELLFFISQFFKIPKTQIELIKGKSSRHKLIRLPLSESVFCFLNNPKI"
BASE COUNT        10937 a         7765 c         6175 g        10149 t
ORIGIN      
        1 aaaccccaag gtcgaggcaa aaaaggtttt taatgaggga gtttagataa actaaatgac
       61 tgaattaaaa acctttttta acaaagagat tggggtttag cgtaagccct gatcatgcaa
      121 ataagtcttg caaaaacaat tgagtgcttt gccaggaacg ttgatccgct aatttattgt
      181 aatgcagtcc catttcatca tcattcgcat tagggtcagt aaatgaatgg gctgtttggc
      241 cataaacatg aacttgccag tccactttcc tttccgtcat ttcaatagcg aattggttaa
      301 cttgctcgat tggtactaaa gggtcatcat aaccatgcaa tatcaatact ttcgcgtgca
      361 atggcttatt actctcaccc tccaaaggta ataatacccc atgaaaactg acaactcctc
      421 taagcggtgc cccggaacgt atcaaatcca atacgcacaa cccaccgaaa caatagccaa
      481 tagcagccac tttatcacta tccacctcag gcaactgaac cactttgctg aaagccgcat
      541 taatacgagt gattaacttt cctctatctt ctctgaaagg agtcattaaa gctcttttct
      601 ctaccttatc cttgcctagc ttggcatttc catacatgtc tatagcaaat ccaatatacc
      661 ccatgccagc aagctgaatc gccttgttac aaactccctc cccacgcccc ccccaatcat
      721 gagcaaccat aacgcaaggt aacggttttg agacactgga atgataggca ataaaaccct
      781 tgcacacagt atcaccgtcc tcgtattcca cttccttagt cactaacata gtaatcctcg
      841 ttttaaaagg ccgtccaaac agcattaata actaactgtc aaagccttaa tacaatgaac
      901 tgctcataaa taataagaaa ggctcctttg agcccttctt atagtattgt tctagaatgt
      961 tattcgggag gtggtgtact ggtcggggta ccactgtctg aaccacccag ttgacctgta
     1021 tccggtttgg agcttggccc agtctggctg ccgctgttct tacctttccc tgaaacatca
     1081 cccgcatttt ctgaaatctg tttagcttgg ggtccaaatt tgccaagcat ttcttgccct
     1141 ttagctccca atttgtctcc aatagctttc tcagcgccat aagtggcttc acctgctttc
     1201 tctactttac ccttggcttc ttcaccccat tgcgtggttt cctgaccaaa agattcagga
     1261 ctaccaccta tccatctcaa cactttatca ggcaggtgag caatcagcgt aaaggatttt
     1321 tgtacaataa ttaaatacat ggtggtataa attaatatgg agaagaagaa agcatacact
     1381 cctgcccagc ccgaataacc gccctgcaat gcagttgact gataatcacc gctcaatttg
     1441 gatccaaaat catcccattg cgatgtgtct attttcgacc ctgtccaatt cgcgaccgtc
     1501 tgccctgcct gtatcatttt acagtcagca ggatacattt gacagtatgc agaattagca
     1561 tttttgtctg cgaaatcacc ttttttccat gtttctattc cttgatccga ttggatgtag
     1621 gaaatggcat gatcaaatcc ggcattcaaa atccagactc ctacatagga caaggcaatt
     1681 gctgtaatat atcctattat catcagagaa ggtcttaaaa agacattaac caaaatcata
     1741 atggcaaatt cgcctttacc aaaagcctca ttaccttccg gatgcgtaac acccaaagcg
     1801 acaattgggg cagcaaccat agcctcgata acggctatca gccaggcaaa tgatccaaaa
     1861 gtaaaaatca tataaggtaa aacgggtata taatatgctg taacaaatcc tatggatacc
     1921 atggttccta tccatgccat caacaagggc atagccatca taatcaaggc aaagataaag
     1981 ataccaaaaa gtgggattag tgaactgacc acggccatat tcaacagtgt taaccacaaa
     2041 gtaccagaaa aattaatata catcgtcccc atattagcca acgcgactat ggggtttact
     2101 ccaggttgcg caagagtttg aactcccact atgaaaatat ctttcatgcc ttgtaaagga
     2161 atcattaaaa aggccatgac tatggaatta atcatttccc cgaatatagc tagaaagaaa
     2221 ttgtacacgt acctgaaaac atagttataa aataaatcac ccatcatgcg gccaaggcaa
     2281 aaagagaaga acaaaatctt caccctgcca caatcaaaat cctggtgctt catgtaataa
     2341 agcgaggtat ctactttaaa attaataaga ttggcaaagg ttaacggttt tattcccggc
     2401 tggcctggca attggaccat catggaattg ttgataaatc catagactgt tgaagacaat
     2461 ggtccttcca cataagcacg tttagggtca ggacttagat caggctgctt gaccccatcc
     2521 tgactcaagg ctggtgcccc attgattaat gactggattt gaaccagctt atcactcttg
     2581 ttttgaaacc agttgcacaa taaagcatac gtgccctgac acgttttccc aaatggcttg
     2641 gtgagttgtg ctggatcaaa attactatta tcaaggcctg tgtcactatc aaaatcactc
     2701 tcgtctttta ctgcacttcc attgagtttc accaaatcaa aaaaataaga gccagccatg
     2761 atccaccctt tggtattggc ttcttggata aaatcacggg atttgtcatc atattcagca
     2821 gatttagatt gttttatcag attaatggtt ggcgtcataa tgccattgta gtcattaatc
     2881 gcccctaaaa attcagtacc attaaataat acccccgtag tagatccggt agaactgggt
     2941 atcgatcccc atgtttggca aacttgttga tactcagtgc agacctgacc agatgcctta
     3001 taaggtactc caaactgctg cttggcaatg gctgaaaaat cattttttga gtttccggta
     3061 gacgaagttg atgaaaaggc cggatcatta ttaaccatca cctgtgccac cgtagataaa
     3121 gtcacataca tttgctgtat agcaatcgcg cgagacaatt gagctgtttc caactgggaa
     3181 gaagtaagct ttatgttttt tcccatatca gccttcaaat cagaaatact attccaagtt
     3241 actgtgccgc aaatcccatt taagaaataa aatggtgagc ttttatcaaa gtttggcata
     3301 tcaaggctaa atggtccttt agacttgtca tcgctttgct tcttgacaaa attcactgtg
     3361 ctgatgaagt caggaatcgc tgtacggcaa aaagtattca tttcaggagt aggattaccc
     3421 ccacaaggag ggctgttact ttggctcaga taaagattcc tttgcgtttg taattgtttt
     3481 tgtaaaccca acatgcagac ttgaccagac aatatagtaa atgccccttt tgctatacct
     3541 gtagacttcc cacctttatc tgtgaggctc ttggttgggt cagactgagc ctgtattatc
     3601 acaccacccc gattcagata actcaaggca gcttcccata ttttatcagc tgcaccaaca
     3661 ccttgcacaa taacccacat aaaaaatacc tgcatcatac aataaccaga tgctttaggt
     3721 attaacaaag ccaaaccgaa ggtggaacga agaggaatcc agattgagga ccatttttgg
     3781 ccaagcattt gcccttcatg ggctgtgttc atggtggaaa ccatgagggt atacataatg
     3841 atgattccac ccaaggcaag gacagcggag ttgaagacac caaacatgtt gcccattatc
     3901 tggcttcccg ttccgtgcag taccccatca actacaccaa acaggttacc taaaaaaacg
     3961 acggataaat cgcttgctgg aggcgcgaaa cttaaggagc cactttctgc gagagcaagc
     4021 gcaggaaaaa agcaaaggag gaccgtaata gctaatttat tcattctttc tcacccaata
     4081 acccttgcct gtaccattct ttaacggtac atcccaattt acgttgttta atctggtagt
     4141 accaaaaatg ataacgaaag gctagtacca atgcgatcat aaccacaacc aaacttatta
     4201 ttgtagcttt ccatgaacca taaaaaagtt gataacccgt gtaaatgagt agcagaaaag
     4261 cagcgataac cataatcaca cttagccgga acaaggcttt ttgtttgatt atcaagtcag
     4321 tctcattcaa ttttagtttc ttgactgctt catcaaatga ttccacatgg gttggttcct
     4381 gtgggacgaa taaacgctta acgccattga ccaaatataa ggtgaatgac ttcattctgt
     4441 cccaatcgac ccatttacga acattgataa tccttgtaat aactcttacg atccttgatc
     4501 ccgatttctt tttcatattt tttacttttt cacaatttta agtaatggac ttttatatat
     4561 cataatatac tatagtatta agtagttgtt tcgtctatca acgtatctca gtaaaaaaag
     4621 agtaaataat gcctgattta agccatgaag cctccgcaaa gtactggttt gagtaccttg
     4681 accccatgat ttacagggtc atcactttta tggaaagtgt ggaaaactgg actcttgatg
     4741 gaaatccgga actggaagag gcaatgaaac agttagggca agaacttgat gatattgaaa
     4801 aaattgactt gggcttgcta gcagaagaag acaaatttat tcgtatcgtt ggaaacatca
     4861 aatcaggaag aggcttacgc ttgcttcagg caatagatac agtccatcct ggtagcgcct
     4921 cccgtgtttt aatccatgcg gaagaaacca gtttaagcag caatgacccg gcaggttttt
     4981 ttctaaaacg caatattgta tttgaaagac taaggttatt atcgcgagtt ttttgccaat
     5041 accgtctaaa actcgtttta cgagcactcg aaggggatga ataatgaaag tattacctaa
     5101 actggccttg gccaatctgt tttgttttac cactttatct gttttcgctc aaggacaagg
     5161 atatgatcca tcgtaccagc agacaagcac caatacgtcc aacctggtaa cttacctgac
     5221 caatctggga aaatatttag gctatgacat cactcaatcc aataaaacac ctaacccgcc
     5281 ttattcacaa ttattcaatt ccaatgtagc ccaactcatt caaagttacg cttacaatac
     5341 ctttttaggc gctattcctg tcgatgccat gtcacagagc ttaatgaatt tcgtcaccaa
     5401 taatgtgcaa ggaaatgcat taataaataa cttggcaaac agcaccttta aatatcaaaa
     5461 tttcagctcc ccatcctccg gcgcggatgg gaaaattaca gctaacggca taatagacca
     5521 atcaacaacc actgcccaag ctcccactcc cacggggatt ttttcagctc cacaagcccc
     5581 aactgccgga acttatttga atgatccggt cagtcaggcc gtattcaata ttctgggaac
     5641 cccggactac agtttttgta tggacaacga tcaaaaaaac tggaaatccg attgtaaact
     5701 caactatcaa aatctggtca tgcaaaatgt tatcggcact ttgcccccgg cgctatctca
     5761 agcgggacct gttttttata gctatgaata caataaaccg attattagcc aactaaacag
     5821 caattcactc attgccccat tactcttgga tacctcttcg tcacaatctg ggcaaaattc
     5881 tggattaacg gcaaaaagcc aagcccagca agcgcttaat tttattagat acgcaagcgc
     5941 tcaagtcact cctccttcct tacccaagtt gagtgcctac agtgagttat ggaatcaagc
     6001 cactgccaaa ccgaattctg aaggttataa tgaagtccag caaaaacaag cggcggcaac
     6061 attgagcggt tattttaata atttgcgcgt ttacgccgca caaacttctg tgggcgtaag
     6121 taacctctac tatattttat ccaaacggct accccaaaat atgagtggag atcaaaatag
     6181 ttccaatatc accagccagg ctttgaatga atttaacatg gcaactcgac gattatttga
     6241 tccaaccgcc tccaacactc ccggacaacc aaaccaacaa tggattaaac aaattaatga
     6301 cgcatcccct gccacagttc aaaaagaaat agcggtattg ctggctgaaa ttaattatca
     6361 aatgtatttg gacagacaaa tacaagaacg gatattgtta accaacagca ttatgctatt
     6421 gcaaaactta aaagcagctc aaccaaccgc agatttttca aaccaaagct ctccctctga
     6481 gcaacaatag tcaccacttt ggctcctggg attccaattc cagggagcca ttgtaaccag
     6541 gtttattaac acgcttaaaa atttggtaca caaaatcaat catttttttc ccttgcggtt
     6601 ggtttttgtt taaataaaac cccgaattta atgtgctaac taattgatag tttttaaaaa
     6661 aatgtttttc atatagcaaa tctgttaaat aatcacctcg tgattccata gggtagtaat
     6721 tggtgatcac ccaatcaggc ttcactttag aattgagata atcggcataa agacttaaat
     6781 cgtttttaaa tggtttttgg gttaaatcgc tattgttcaa acattgcgcg tcgatgaatc
     6841 gaatgtcttc tcgagaaaaa aaaggaataa tcccacaatc atctaacaga acgctgtcgc
     6901 ccctttttgc ctttcggttg agcaaatcag caatagtcat tctattttga gtgcgttcat
     6961 tatattctgc aactgatttg gataaatgat ttaaatcatt cccgggcaca aaaaaccagg
     7021 taataaacaa cattaataca gcagccagat attcaggatc ccatctgaaa aatttaaaat
     7081 attgtatcaa ttgattaagc cctaaaacag gtaatatgga aaaaagggca aatgggctta
     7141 aaaacaatct caaaccgtat gtgactacag gatttgcttt ccaaagcaac aaagtatata
     7201 aaacagaagg caaccaaagt aaaacatggc gacaatcttt attggcagac aataaatagg
     7261 gtaccgataa tacaagcaac ggtgttataa aaagaaaata atcaaaatct actgccaaaa
     7321 actgcccata agctaacgct ttgcaacgat agctattagg aatccaatga ctgaaataaa
     7381 ttaatcgcca aataaaatac acaagatagg gcaatacaaa gcaaataaaa gtagttgaac
     7441 cccataagaa caatctctta tgatttttaa aacccagctc tctttgagca ctcaaatatt
     7501 gacatcctat aaaaacagcc aaaggaatta tccaaacgat tccctcaaat cgtgttaagc
     7561 tcaataataa caagagcata ttggttatta tccaatgagt ggttgagtaa agggaaaatc
     7621 gattgggcca tggcctgtca tcaacagttt gataaccgaa tgccaaggta cactgccata
     7681 tcaatgccaa agacagagcg cagtaaaata aggtttctaa tccactgacc gcccaccaag
     7741 tcactcctgt ataatggcta aataaaaaaa ccggtaatat agataacaga gggctacaaa
     7801 aaagtcttgc cagtcgatat aaaaacagta atgccaatcc caagctacaa caggaaatga
     7861 ccttcatact gctcaccaga ggtaattgca atttaataac caaggcagca atcataagcc
     7921 ataaaaaatt ggaatacccc tctacgggag gcagggtcgc atgccaaaac agccctttgc
     7981 cgctcactag ctgtcgcgcg taataccagc tgatataagc atcatctgtt gtgaaatccc
     8041 aagctgtata caacgcgacc atcagatcgt aagcgcaata aatggctatc aatagcacca
     8101 gaatggtttt tcttcttgat aacagggaac tcaaaatgtg ttctgctcca gaaaattttt
     8161 ctataacaca atccacaata gcaatatcac aaagtatcaa caagctccaa aatcatgatg
     8221 caaaaaaaat aatgggtatg ttatgattcc aacccaatta aaaaaactgt atcctcaatg
     8281 gaataatacg gaacttacaa gaaacgccaa aagtctgggt cattgtgata agggagtacc
     8341 acaataacat ttcaaaacag tgaatcacat ccactggact aaagatgata gaccttgatt
     8401 taaattaaaa atgtaacccc tactcaatgg ttggtgttga ttgataaatc cggtacaaag
     8461 ttctcaggat gagttaacca tttaactcag cgagcgatta tgtccaaacc ttattatttt
     8521 tatgtcagcg gaatgaattg caccagttgc agtaatgcca tagagtcctt tttaagaaat
     8581 tccaatttaa tcaaaatcaa tacatttcat gcggacatga gcaccccgga tccaaaaaaa
     8641 attgtgatat ctctttctga agaaaaggaa aatgaacatg aaagcaactg gaaaaccatt
     8701 aaaaaattaa ttgatgatac aggttttact tgcgaaaatt atgaattccc tcaggtgaga
     8761 gatcaaaaac ctgctcttcc tctcgagaaa acgcagacaa ccgttcaaca ggaatccagc
     8821 caattatccg cactctttag cagcgcaaaa aaaataatca cctcccattg gttgcttgga
     8881 ggattgggtt gtatcactgg ggttagctta atgatcctga ctatggctct aggtggttta
     8941 ccacttgcgg taatgattcc tttagcaagc atcagcactt tattaactat tgcgcttggt
     9001 gcccaatctt attacgaggc ttggaagaaa ttagtccaat ctcgcacact cacaatggac
     9061 acattgtttt caattagcac catttctgtt cttgttgtgt cgcttgcttc attctttgta
     9121 ccgtggttac ctatgatgtt tgaggcagga ttacttattt atggattcag gcatattggt
     9181 atcgctattg aggacactat caaagacaaa ataagctccg ccaaatttca agacagagcc
     9241 ccgcaaaaag tcagattata tcgtcatcct gattttaaaa aaacgaggat tgatgagatt
     9301 caacccaacg acattataca agtcaaaccg ggtgagctta taccattgga tagcgtttgt
     9361 gagcaagaca ccctcattta tcccaccatc attaccggtg ctgtcatacc taaacaattt
     9421 cgtagtggtg aaaaaatact tgccggaatg cgtctggcaa atgatgcaaa accactcagc
     9481 ctccgcgttc taaaaactgc aaaagagtca taccttgccc gtcttgatga aagcatagct
     9541 caatctgtac ttgaaaaggc accgatagag gtaaaaacag ggcagcttct tagctatttt
     9601 atcccatcca ttattgctct ctccatcctt tcagggatca ttgtcggctt gattgccaca
     9661 ccagcacttg cgatacaatg cgctatttct gtattggttt ctgcttgtcc ttgtacgctt
     9721 ggtttaatta ttcctctagc agtaaaaact ggaattcata aaggagccga acacggtgtg
     9781 cagtttaaaa attccagggt attacaatta gcagaacaaa ttgatgtagt catttttgac
     9841 ttgaatggca cgttaacaac cggcattcct gtggttaaag aatatgctgt attaaatgat
     9901 gctgatcttt cttctgagca atttctgagt atttgcaatg tgttggaaga acaatcaaaa
     9961 cacccgttcg gcaaagcaat acaatcgttt acccaaggaa tcaataaaca aaaattcaat
    10021 attgctaatt tggatcattc gaaccattca ggcatttcgg cagagattaa tggtattcaa
    10081 tatactattg gcagtaaaac cttaatgcag gaaataggga tttctacatc ttcgcttgaa
    10141 aaccaaatga atctggttgc gggcgatagc ctggtgtttc tagccagaaa caaacaacta
    10201 ttaggttatc tggtcatcac cgacccctta cgaaaagatg caaagcaaac agttgatgct
    10261 ttaattaaaa tgggaaaaga agtgcatcta tgcactggtg cagatgaaaa aaccgcctat
    10321 cgctatgcgc aagctcttgg cattaagaag gtacatgcta attgtgtcgc tacaactatc
    10381 aatacagaag atagatccaa accagtttat attaaatcat tacaaaatca gggactaaaa
    10441 gtagctatga ttggagatgc ggctaatgac gcaaacgccc ttgctgcgag cgacttcggt
    10501 atagctgtac tgtctcaaga cagcgatgaa atcacccagc aacacgcctc agccattata
    10561 caaagtggaa ctatattggc tatagccaat gcctttgcca tatcaaaaca aacagtaaac
    10621 aatatcaacc agaatttgtt aattagctta gggtacaatt tgacaacagt attaatagcc
    10681 ggtggtctgt tagtcaccct tggttttgtg ctgaatcctg ctgttggtgt ggcattaatg
    10741 gtaacccagg cttgttttgt cttgctcaat gtataccgct ttaaagagca acccttgcaa
    10801 catttgcaag acaaagttca ggaaaaacca gaaccggttc cttcttctta tgaagccatg
    10861 aaaaagcatt cacccaattt ccacacagct cccgagatta atcataaatc agtcgttcaa
    10921 tctggaaagc agccgaccaa caaaaataaa tcaccttcct tttggagtga ttattttcct
    10981 gaattatttg aagaagaaaa ggacacaggt accgaacacc taatgaataa caaaatagta
    11041 taagccctaa ataaagcttg gctgaatttg aaatgaggaa ggccaatcac cttcctcaat
    11101 aataacaatc aattttgatt tattatgctc taaagggtca agaatgtgat gtctcttacc
    11161 aatatatgat gactcataaa aatcgcatgt aaaatgaggt aatcagcaat gtataccgca
    11221 tcattatata gacaataaat aaattatatg gtcaaattgg caaaattaat taacttacat
    11281 tttaagaaaa ctcatgccaa caacctttga agataaatca tccaaagtcc ccagtttaaa
    11341 atccctggcc gctaatgccg ttcaaaaagc caatgcaaat ctctttttca ggttgcactc
    11401 gcttgaaact ccccctgaga taaaaaaggg gtttattgac aaggagctag aggcattaac
    11461 ccacgaatta actgaagact atcaaaccaa ggtggaagcc aggaacgaaa aaattgagga
    11521 atgcagttcc aatcttagtt ctaatgaatg ttttgtaaaa tgctctgctt ttaccctcac
    11581 tacattgatg gctggagtgc acgttggtat ttattacatc ctgaaagcag cagctgtcga
    11641 tccatcaacg caaatagcat atatttcgag tattcccgct actatttgtt tctctatgtg
    11701 cgttggagtc tgcttgaatc gacaaattac taaatgcttg agttcttgtt ttacgcccag
    11761 tgtccccgat aaaattactg ttgatttgga tgagttgggt agacaatccc atgttagtcc
    11821 ataagcggct ttctgggttc cttgaaagaa aaaactgact tattcgctaa aatataattt
    11881 aattacaatg attcttcact ccctggtaat tgttaaaaaa tagggagtgg tttcatacac
    11941 ccattttcca ccgtattttt ttttgcagta ctcatcaaaa aactgcacca tgctgtttac
    12001 aatatcttga tacattttac cggggagatc cttgaataat ggggcgccgt gtataaagtt
    12061 ttcaaataac ccgagatcag gtaattccac ttggcaatgt tttacaataa aagaaatgct
    12121 tttaaatggc aaattatcca atgcccttcg gcattgaaca gaataattct cccgcatcat
    12181 tttagattgc acgacatttt tagcgacatc agggtaaaga ttttgaaatt gcaggctgtt
    12241 taacacttca aacaaagtgc tttgtttgcc agaagaaaaa attgcacaaa ttttacctcc
    12301 tggtttcaag acatgatata aattggataa tgatttttcc aaatcagtcc agtgaagaca
    12361 ccaaaatgat aaaatattat caaatggtcc gtaaattata ggttcttgaa tgtcccctat
    12421 atcaaaacga acgttaatac tcctgcaatg tttgtttgca taatcaatca ttgtttttga
    12481 tcgatccaag ccaagaacat atccatcagg gactaaattg gctagcattt gagtgaaact
    12541 gccatcacca caaccagcat cgagtaagta atcattacta tggatagata tctttgcaag
    12601 aaacttgctg gagatttctc ttaggatata gttacctttt gcataattgg ttgcagacca
    12661 attattcaat atggcaagca aactttcatt atccattcgg gatttcttcc aaaatgattt
    12721 accaagagcc ttttgtaaat tatagagttc tccaacacaa ttttctaaaa attggaagct
    12781 tttgatgttg tattctctga gatctctaaa ctagtgatgt tataaacaag gaatcccatt
    12841 aggccattgc aaccaatttt tctcaggata aggtaatgaa atatcaacca aatgcaaagt
    12901 ataggcatgt ctggattgat tagaaaaatt agcctggctt ttatgtggaa ctctgccatg
    12961 tagtataatt aaagcgcctt ttttaacagg taaagggaca ctgttttctt ctggccaagg
    13021 acttgaatcg aaattttcaa aataaatttc gttttctcga cgaaacatcc tctgctttaa
    13081 gggtgtgata caagggcttg gaatcacttc caaacagcca ttttccaaag tagcatcttc
    13141 tattgcaaac caaaacccta aagcatcact atcctcacca aaaatatagg tactatcctg
    13201 atggcaaagt acttcggccc ctattcctgg ctgtttgaat atgtacatcg attgtaccaa
    13261 tccaagtgtt ttcaacccaa gctgatgtgc tatcttctta attcgatcat ctcgggaata
    13321 ttgcctaaaa acaggatcca attcatgtaa agcatgtcca attttattaa ttgattgatt
    13381 aaaaggacgt agacaatcac cggcttcatc aaaagcaccc ggctcaaaaa aataatgaat
    13441 tttatcccca gaatcaagga aatattgttt ttttgcatgc tcgttggttt gcgtggaaaa
    13501 aattgtttta ggtatctctg cctgattatt ttcaagcaat gtttcaatgc gttgctttaa
    13561 tagatcgcat acagactctg aaaagaaatt atttattacc aaatatccat ttgtgaaaaa
    13621 aaaatcacat tgctcagaag aaagttgcat cctatgctcc tcaaaaatca aaatcagtac
    13681 ttggggaaat caccagggta aaggcaaaca gcttttaacg agcataagcg aaatgctctc
    13741 attaaaagcc tttcccacac aaagattagg tattgttcat aagtcctgaa aatatttcag
    13801 ttaatttttc cattacatca tctacggtaa ttaatttcat ggcttcatat ccatggacct
    13861 gcgtacccca aacactcttc tctggactca ctcctaatac agtttggata gcttgcgaat
    13921 acctgttaac taccaaatgt tgaaaagtat acgggccaga aatagaggga ttggtaacag
    13981 catgcaaagc cacaacaggc gtgcctaccg ctgccgccat atgagaaggt cctgtatcag
    14041 ggcacaagac tgcttttgct ttactgatca cagccaacaa ttgcttaggt tttgtctttc
    14101 caaccaaatt aaccactgga atttctttcg ctatttcctc tgccaatgat ttatcatgat
    14161 cacctggccc tccagttaaa acaactttga catcccattt aacttgcgcc tgtttcagaa
    14221 cctcaatgta tcgctccgcc aaccaactgc gctcaggttt gctggcggcg ggattaacga
    14281 ctaaaattgg cctttcttgt tccggcaaat gattttgagc ccattcataa tctgccttgg
    14341 taatcggtaa atcccagtgg attatttttt cagtgacacc taaaggctca gcaaatttta
    14401 aaaagccctc aagtgtgtgt tccaatccag gagtaatgct ttccttgata aacagacgat
    14461 ggccatcatt cgctctgtga gaatcatagc caactttgcg tttagcttta ataaatggaa
    14521 taagccaatt agttctaaaa ctggcttgag gcgctaacaa cacatcaaac tggcgaggca
    14581 acatttgttt cctaaaccgc caatagtcag caagcgtttt aggtttatca ataacaataa
    14641 actccacccc ttccattcct tccatcaaat aaaaggcagg ttttgaaata atccaggtca
    14701 attgagcatt gggcaattta ttttgcaagg tccttatcag tgggaccaac atcaaaacat
    14761 cccctaaagc tgaaagcctt ataacacaaa tcgatttaat cattctgcaa tacaaaacga
    14821 gagccacaat aagggcaaac ctccaccccg gttttctcta taggcaaata aaccttggga
    14881 tgagcattcc ataactccat ctcatctgtt gggcaactca atggtaattg atgtctatgt
    14941 acgacataat tttttttagc acttgcaggt tcttttgttg ttttcctcat gttgttcctt
    15001 tgcggcatga tgtcaattag agacagtatt atctaatatt tcccttattt tcgctataat
    15061 atctgtatta aaacccacat tttgcgaatc ataaaataaa gcgccctccc aatgcctcaa
    15121 ccaggttaat tgccgtttgg ccagctgcct tgttgctgct ataccttttt ccctcatagt
    15181 ctcataatca taatggcctg caagatactc gagtatttgc ctatatccaa cacatcgcat
    15241 ggagggtaag ttgatttgaa taggccattt tgcttgcaat tgttgaactt cttctatgaa
    15301 tccctcacta agcatcgcgt caaaccgttg cgcaatacgt tcatgcaacc aggatctttg
    15361 ctcaggaaaa agagcaagat taacaaaata ataatcaggc tgtacatggg gtccggtcaa
    15421 ataggttgat aacgttgaac cggttaagta ataaacttcc agcgctcttt gaattctttg
    15481 cgtatcatga gcatgaatgc gagcggctgt taccggatct aattgactta gcttttgata
    15541 aagaaaatcc caaccctgtg ataatgcttc ctcctccagc cgtttacgaa cagcttcatc
    15601 ggcttcaggc aaagtggcta accctttttg cagagcatta aaatacatca tagttccacc
    15661 cactaacaaa ggaatattcc ctcttttgat gatttcagca cataaagaca aagcgtctgt
    15721 acaaaactgg gctgccgagt aggattcaac aggatcttta atatcaatta aataatgagg
    15781 cgctctctgt aattcatgga ttgaaggttt tgctgtccca atattcatat ctctataaat
    15841 catggcggaa tcaacactaa taatttcaaa agggaaatgg gttactaagt cgcatgctaa
    15901 ccctgttttt cctgaagcag ttggtcccat taaacaaaac actagcttat tcatgcaata
    15961 acatccgaca atcatcaatg gttaacgctt tacatatatt atgtttctct tctttaagag
    16021 aaagttccag caacaattga ttcatttcaa tacgctcttc aactgttaat tgttttgggt
    16081 caaatatttg agaacggcta attaacgcta tcaattgctc agtatgaaaa atatccaatt
    16141 cacagagaac attaaaaaaa caacgaacgt ctaaataggg cgcattcaaa gggatgctgc
    16201 gaattatcaa gtcatcagca ttagggtact caatttgtat cccaacacga gccaactcat
    16261 gctttaattg ctcctgctta tgcatccatt ggtttggtaa tgggtaacga attgaaatca
    16321 ataacggcct gctctcaaga ggtaatgcct tttgcattag ctgtttttct atccattctt
    16381 gaaacaatgt tacgatgtct gcgaggtaag gctgttgctt tataaaaacc aaaataaacc
    16441 gctcatttaa gataacccac tggaactctg tttctggatg atatacagag ctggtttttt
    16501 tatccctttt gttggttata gatgatgata ataagttggc actccagctt ggggataaag
    16561 ctcttgcccc ttcttcatgg gactctctgt cattgcagat atgggaaggt tccgaaactg
    16621 tattttgctg ctcattacaa gaggaagcag catacgcgta ttcccttgta tctgaatggt
    16681 caagagtcga ttgcaacgct ttcgtcaatt gggaagtaaa aaaatcatgt accagacggg
    16741 gctgttggaa tctcacctca tgctttgtag gatgcacatt cacatccact tcagaagcag
    16801 gtagagtaaa atacaacaaa caaattggaa atctgcctgc atgcaataag ccatcataag
    16861 cttgttttac tgcatgttgg agtaatttat cctttaccat gcgttgattc acataaatcc
    16921 actgcctatc attctggctg cgttgcaaac gatggttgct tatccagcca taaagacgca
    16981 taccactgtg ctccacatca agaaaaatag cttctctcat gaaagtattg cccaaaattc
    17041 gacccatacg aatggctttg gcttgctcac ttaacgctgg tggtaatgaa aaaatcagtt
    17101 tcttattatg cttaagcgtt aatgcaatcc ggggagcact taatgcaaac cgtttcacaa
    17161 ccatttcaat agcttgaaat tccagttttt cattttttaa aaaccgcttg cgaactgggg
    17221 cattatagaa caaatcagaa acatcaatgg tagtgccaat attgcgcgca caaggagaga
    17281 gagtcctgct ctccccctct actcgcaaca tcatcgcatt gtcctgctcc tcaggctttg
    17341 aaatgatagt cactctggca acagaggcaa tacttgccaa tgcctcccct ctaaagccca
    17401 tactatcaat ggaatataaa tcatcaagcg ttctgatttt actggtcgca tgagctgcaa
    17461 cagctaaagg caagtcgtct ccaaagattc ctgaaccatt atcactaact gtaatctgca
    17521 gcaatccccc ataattgaca tcgacaccaa tcacactggc accagcatcc aaagagtttt
    17581 ccaacagctc cttgactacc gaagcaggcc tttctataac ttctcctgct gcaatctgat
    17641 tggctattgc tggcgaaagt tgatgtatcc ttaagaccat gtgccaggta tcactaattt
    17701 ttgcccgggc ataagccttg cccttgcctg aaggcggtta attgattgta atgcgctcac
    17761 agatacccta tatcgagcag cgatagcagg aagagtttca ccctttttaa cgatatgaaa
    17821 atttggagaa atcatagcct caattcgagt cccatgagga ggatgatccc aaaaataatt
    17881 ttttagtcct tgaaatattg cctgactcaa tcgctcctgg taggcagggt tagttaaatt
    17941 tctttcctca cggggatttg agataaaacc ggtttcaacc aaaatagaag gaatatctgg
    18001 agatttcaga acaacaaaac gggcttgctc cactttgtta ttatgcaaat tggtaaaatt
    18061 atccaattgg cctaatactt ttccccccat ttccaaacca gcatttattg tagcagtttg
    18121 tgataaatcg atcaaaacag agcgtactac cccattacta tcatccaact cacctaaatt
    18181 cactccacct agttcagagt aattctcctt ttctgctaac cagcgtgctg cttcactggt
    18241 ggctcccctt tgtgataagg caaataccga ggccccatta gagtgaggat tattgaatgc
    18301 atcggcatgg atggaaatga atacatcacc attatatttt ctggcaatat ccaggcgttg
    18361 tctaagtcca acgtaataat ctcccgatcg tgtcaggact gcacgcatac ctggttgcct
    18421 gtctatcaat cgtttaagtt ttaaagtaat ggccagcaca acatccttct cgttactacg
    18481 gcgcggacct cttgcgccag gatctttgcc cccatgccct gcatccaaca ctacaacaac
    18541 atcacgcaaa ggtttttttg atgggttatg aggaacctgg acaggtgttt tatgcacagt
    18601 aattcgttgt tctgatggct ggctggatat ttttgattgg atttgctgga ctggagcttt
    18661 aatcgtttta acaggcgggc tcaactgcct gcgattattt atgactaaag gagtatcatt
    18721 aacaagatca attctgatcc cattaaattc tcctttcggt ttccatgaag atgagcgcac
    18781 cttgactggt tgttttacat ccatgacaag cctgagcgtg tttttggagg gactaccgct
    18841 tcttacctgc tgaataagcg aattgattaa acccaattga ctgatattga cggctaattg
    18901 tgtatcactt aagtctaaca ccacgcgatt gggctgactt aaggtaaata gcttatgagt
    18961 aaatgaacct tgtatagaaa gataaagaga cgtcatggtt ggttgctgag aaactgtaat
    19021 attctttaat tgtgcagaaa atacagcaaa agaacttggt agatataata acagacttaa
    19081 gacccacgct cgcatcatca tcattcaccc gccaggcaag ctaaaattct tttacccgcg
    19141 acacttaatg cagctatttg tattaagcgc ccagtccctt taattattaa attaaactga
    19201 atatcgactg gaggcaatgt ttttccacca tgttcaggcc attcaataca acaaacgctc
    19261 tgattggaaa aatagtctct aaaacctaaa tactccagtt cttcttcctg ttgaatgcgg
    19321 tacaaatcaa agtgatgaat atgaaattga ccacaattgt aactttcaac taatgaaaaa
    19381 gttggactct tgatggctga aattaccccg agtgatttta gcattgcgcg aacaatggtg
    19441 gtctttccgg cccctatatc accacttaaa gtaatcacca aaggcgctga gatacaaaac
    19501 gccaattttt ctgcaaatct tttgctctct tgttcactaa ccaaatccaa agttatgaaa
    19561 ttatcattat cttcaatagt catatcgaga acactacata atgtatctgt ttagaaaaag
    19621 cctgtcagcc aagccgttat aaacaagcat tgaaacagag cttgttacct cagtaacaat
    19681 tcagtttacc ttcttaatga ttcatcgcac tagatacaat tcaaaatgct tggaatttca
    19741 tcaagtaagt cgctagccaa taccccaact tctcctgatt tttcagcaac cttgtctcca
    19801 gcaacagcat ggacccacac gcctaacttc gcggcatcgg acaaggtaaa accctgggcg
    19861 caaaaagcag caataacccc actcaaaata tcccccatac ctgcggtaga catacctgga
    19921 ttgccttgag ggcacacaaa aatattcctt tccgctgtct gaataacagt acctgctcct
    19981 ttcaatacca caacaccacc atattgtttt tgtatagcag atgccgcatg gtatctatcc
    20041 atttgaacat ctcttgcagt acatgaaagc aaactggcag cttcgccagg atggggtgtt
    20101 aaaatccaat tgtcatccat ttggggatgt tgagaaagta acctcagcgc tgaagcatca
    20161 atgatcatgg gtaattgggt tgtaattgca gttaaaaaca aggttttagc ccaatcacta
    20221 tcaccaagcc caggcccaat aacacaaacc gtagcttttt ctaataaagg cattaactcc
    20281 cctgctgttt tgatacccca gatcatcgct tcaggaatta agggtaaagc gctcttcgca
    20341 taatccggcc aggttgcaac agtaactact cctgttcccg tcttgagtgc tgccttggct
    20401 gccaagctca ctgctcccgg cattcctggg cctccaccta tcactaaaac atgtccataa
    20461 tcacctttat gagagttctt ttttctagct gcgagaggca atggcaaggt agaatccctc
    20521 agcagggaag caaatcgggt taatttagtt gtacatgcct ccaggtttaa atgagcacaa
    20581 tgaatcaaac cacaataatc tggtccatcc aaagtataca ttcctgtttt aagtccgata
    20641 aaagtaattg tggcagtcgc attgacgcaa atattctcta ctaatcctgt atccgcatta
    20701 agaccagatg gaatatcaat cgataatacc ggtaacccac tggcattgat ttgatgaatc
    20761 gcgttggcta ttgccccatg cactgctcct tgtaatccaa tacccaataa tgcatcaaca
    20821 attaattcgg catcaccatc taaaggttca tctgcggatt gacaatgtac tccagatgtc
    20881 aatgccaggc gtgcggcatt cctggcagca gaaggtaaat cttctattga tttacattga
    20941 tagacagtaa ctgagaatcc ttgttcatga gctaatctgg ccaagacata accatcacct
    21001 gcattattgc cagcaccaca gaagacagct atatgcctta catgcgggta gagtttctgt
    21061 aagaaagaaa atgcctctgc gccagccaga gccatcaagg cattttcgtc tttttgatac
    21121 aaagttgcag cttgctgttc acaagcttta atttgatcac aacgatacag agcattttct
    21181 agcttgttca cctcttggct ccaaattaac gtaaatttga tataacgacc acaaaatatt
    21241 tttatatcgc tatgccaaga tcatgttgta aaaatcactg cttcttggct acgattcact
    21301 taaaagaaac catgccgtct actttaccaa ttttagtgat ggcttatttt ttgtagcact
    21361 ttcagaggaa gaagaagttt taggtggtgg ctcatcatct tcgtcaactg aaaaagcaat
    21421 tcctctgcca ttttcttttg cataaatctc tagtacagca ttgggagcaa caaaaatttg
    21481 ctctgtttgc cctgaaaagc gtgccgtaaa cacaatgcga tcattttcca ataaaagtcc
    21541 tcgggtcgct gagggagaaa tgtttaatac aattctatca tgctggacat gttcccttgg
    21601 aacctgcaca ttgggatttg atgcatctac gagaatgtag ggtgttaaat cattatcaac
    21661 aatccaatcg tagaaagcac gaatcaagta gggtttgttt gatgtcattg ccataattat
    21721 gctgctctta tttgtctctc agcgtccgtc aaactcgttt gaaaagaatc acgtttaaac
    21781 acacgctgca tataggaaat tattccttta aaattaggcg caatttccaa acctaattta
    21841 ggtaaccgcc ataataaagg agccaatgca caatccaaaa gcgaaaactc atcacttaaa
    21901 aaataaggtt tatctgcaaa gacaggatct aaactggtta aactttcaag taaattagct
    21961 ctggcttgct ctacagattc ctcccgcaaa atagcattca ttaaataata ccaatcctgt
    22021 tcaattctat gcatcatttt gcgagcttca gcacgggcta ctggataaac tggtaataat
    22081 ggaggatgag ggaaacgttc gtccaaatat tccataatga ttcttgcttc atataataca
    22141 agttccctat caattaatgt tggaacggtt ccataaggat taatagacaa taggtcggct
    22201 ccaggctcat cttgctttac tgcaagaatt tcaacattaa ctcctttttc tgccagaacg
    22261 atacgaactt ggtgactata aacatcatct acatcagaaa ataaagacat tatggtgcgc
    22321 tttgcaacga ttgccattac cttactccta tcaattcatt taagcctgtt aaaatggctt
    22381 tttatattct agtgcacaaa ggttgctatt ctatcacatt tttaaacttc tgggttactt
    22441 tgagcaaatc ttcttatttt agcgtaaagt cgatacaaga aaccagatat attttctgat
    22501 tctgaaggta accatttgag attgaactct gtataaaagg caaaaaagta gcacctttta
    22561 caaaactgag gagtaagcag tctattccta ttttaattgt ttccaataag ctttcttcag
    22621 tgaataagct gctataagga aaatacagaa gaacaaaaga acaaaaactc ctaatttata
    22681 acgaattagc ttggcaggct cacctacata aaccaaaaaa ttgactaaat ctcttaatgc
    22741 gctgtcaaac tggccttcaa acatctcacc tcgtcttacc actaataaac tatttgcatg
    22801 cgaattttta tctttgacca aaatgacttt tccaatcaag ggttccaaaa cattaggcat
    22861 agcaacatca ggcattagta aattattagc accaaaaggt ctggaagggt cgctgtaaaa
    22921 acttttcaaa taattatata tccaaacagg ccctctatcc cgcgcactta aagacaaatc
    22981 gggaggcacc ataccaaacc attgcttggc atcttcaggc gacatggcta tttgaatggg
    23041 atcataaata ttggcctgag taaaaattaa gttatttttt aataaatctt cagcaatctg
    23101 accatcaaaa gtagttaatc caagatcctg agccatgcgg ttataacgca tgtatttgag
    23161 agaatggcat cccgagcaat aattcataaa catctttgcc cctctttgca aactctcttt
    23221 atcgtttata tcaaccggta cataatccat tgttaaattt tctgtaaaag cagcgtttga
    23281 cattaaaaat gccaatgcag caactaataa cacctttatt ttcatcattt ttcacctatc
    23341 cgtgtaggta ctggccgggt tttctcaaaa cgactataaa taggcatcaa gataaaataa
    23401 gaaaaataaa taacagcggc aattcgagct aaaagcaacc ttactggtgt aaccggagtt
    23461 gtgcccaaat atcctaaaag cagaaaacta aacgcaaaca aggtaatact tattctggaa
    23521 tacattcctt tatatcttac agagcgcaca ttgcttttat cgagccaggg caaaaagaaa
    23581 agcaataata tggctgcagc catgcatact accccaatta gcttatcagg gatagccctc
    23641 aaaatggcat aaaagggagc gagataccaa acgggagcaa tatgatctgg agtcaccatt
    23701 ggattagcgg gtacaaaatt agcatgctct aaaaaatatc ctcccatctc aggagcaaaa
    23761 aagacaaccg caaaaaataa aattgaaaag actatgatac ctacaaaatc tttcacagtg
    23821 taataaggat ggaagggtat tccatccaaa ggttttccat ccttatccaa ggccttcttg
    23881 atatcaatcc cttccggatt gtttgagcct actttatgca atgcaacaat atgcaaaaac
    23941 acgaggatga tcattaaaat gggtattcct atgacatgca aggcaaaaaa tcgctgcaac
    24001 gtcggatttg caacagagta atctcccctc aaccatacag caagaccttt gccaatataa
    24061 ggtatcgctc caaataagga agtaatcact tcagcacccc agtaagacat ttgtccccag
    24121 ggtaataaat aaccaaagaa tgcttctgcc attaacaaga ggtataaaaa cattcccagt
    24181 aaccaaacta actcccgtgg tttctgataa gaaccataaa gcaagcctct aaacatatgc
    24241 aaatagataa caataaaaaa agcagaggct cctgtggaat gcatataacg gagtagccag
    24301 ccaaaattga tgtcccgcat aatgaattca actgaagaaa atgcctgctc cgcgtttggc
    24361 gtataaaaca tggttaacca taaaccagta atgatttgat tgactaaaac tatcagtgct
    24421 aaagatccaa aaaaataaag aaaattaaaa tttttaggtg cataataatt actaaaatgt
    24481 gctttccaag tgctaacaag agggaaacgt tcatctaacc aattgactaa cccattcatg
    24541 ccttaatcct tattttgcat cctcacctat cacaatagtg tggtcatcta aaaagtgata
    24601 aggtggtact tccaaattga taggggcagg aacgccctta aatactctgc ctgaaagatc
    24661 aaacattgac ccatggcatg ggcaaaaaaa gccacctggc caatcgggac ctaactcacc
    24721 aagattaggt ttgtattttg gggaacaacc caaatgagta caaatcccga ttaaaactaa
    24781 aaactcggga ttaatggaac gaaacttatt ttgcgcatac tccggttgtt gatccaccaa
    24841 agaatcagga tccctcagtt gagactcatc ttctttttgt aactggttaa gcatgtcctt
    24901 gcttcgttta attatccata ctggtttgcc acgccattca ataatagcct gttcaccagg
    24961 ctccattcta ctgacatcaa ctttgactgg agcacctgct gcttgtgctt tagagcttgg
    25021 taaccaggaa gtaataaaag gagttaaggc acatgcagcg cctaccccgc ctaagacaca
    25081 ggtagtatgc aacaaaaaac gacgccgctc ttcatccaat tgttcgtctt gattgtgatt
    25141 acttacatcc tgattgagat cagtcatttc gctcactgct taatcatcct gattatggtc
    25201 tatgaactgg atccagttcg cctaatataa ctcccgttag ttataacaaa gaataacaat
    25261 ttttcaaacg gttggataac ttatttctta gaaaataaaa aagcctcgta taaaacgagg
    25321 cttggaaaaa agtaatagaa ttagcgcttg gagtactgag tagctcggcg tgctttgtgc
    25381 agccccactt tctttcgttc cacacgtctt gaatcgcgag ttaataaccc acgtgctctt
    25441 agcttgcgac ggaacgaatc gggacttggc tcagctcctt cagccaaacc atcttcatca
    25501 taagcaacca atgctctggc tattcccaaa cgaacggctc ccgcttgacc agatatgcca
    25561 ccaccagtta cagtaatgta tacatcaaac ttattaagaa gatctactgt ttccaaaggt
    25621 tgcattacaa tcatgcatga agtttctcga cagaaatatt cctgcaaagt tctgtcattt
    25681 actttaatat caccttttcc aggacgtaaa aatacacgtg caattgaact ttttctacgg
    25741 ccggtgccat aatattgctt catttcagcc attatactta ttcctcaatc tcaagttgct
    25801 tcggttgctg cgcagtatgt ggatgttcgc tgccagcata tactttcaac ttgcgataca
    25861 tctctctacc taacgggttt ttgggcaaca tgccttttac agcaagctca ataatttttg
    25921 ttggattttt atcctgtaat ttttcaaagg atgccgattt gatccctcca ggaaaccctg
    25981 tgtgatgatg atacattttg tttttaaact tgcgaccagt cacaataacc ttttctgcat
    26041 tagtgactat aatgtaatcg cctgtatcaa catggggagt atactcagct ttatgcttgc
    26101 cacgtaaacg acgagcaatc tctgtggcta agcgacccaa aactttttca ctggcatcga
    26161 caacaaacca gtcacgcttg acttcattgc cttttgcact aaatgtcttc attaaataaa
    26221 ctccgaaccg cctcaattgg taatctttct atcatggacg ggcgatttta accaaaacag
    26281 aaacaaccta caagtaaaat ggataaaaaa atatatacaa ttaatcaaat ctcaataaaa
    26341 acatcagatt aatgactaaa tgtgaagtat ttttcatctt attgacgtaa gttcatactg
    26401 aatcaacaga aaaccccgat actcattttc atctctttgt ttcattagaa gagactgggc
    26461 ctgactactc gctcagaatt aaccaacgct taattttatc aaaagccttt tcgcgccagg
    26521 aaagcacaaa aaagtttatt tccctaaata attatctgtc tagcagaagt ggcgctacta
    26581 tctcaccatc aattaagtgg tacttgatga tttgttcaat atcttcaaag gaagaataag
    26641 tataccaaac tccttctgga taaataacga tgcaaggacc agagccacat cgtccaaggc
    26701 atcctgattt gctgacccgg atttttccag gaccatgcaa atcaaattct aacaattttg
    26761 atttcataaa atcaaaaaat tcttctccgc cggaattagc acaacattgc ttacccggtg
    26821 ctttctgatt tgtgcatatg aatacatgct tggtgtaatg tgacaacaaa ctactttcct
    26881 attttttcaa ttttcgtttt taattttaaa cccggcttaa aggtaacaac ccttctggct
    26941 tccacaggaa tttcttcacc tgtctttgga ttgcggcctg gcctttgtgg tttatctctc
    27001 aaagtaaaat taccaaaacc ggataatttc acatgctgac cattttcaag agcatgacgt
    27061 aattcttcga aaaaattttc aaccatttct ttagcaactg ttttatttaa ttttaattcg
    27121 tcacacaatg tttctgccat tattgctttg cttagtgcgt tcacgatcat tccctcaaaa
    27181 ggattgaaaa ttcattttcc agtttcttga ttatagcact aattgttaaa ttgatctcgg
    27241 catcaactaa agttcgagta tcgtcttgta gtgtcatagc cacagcaata ctttttttat
    27301 cttctggaat acctttaccc atgtacacgt caaatacgtc aaacgatttt aaccaatcct
    27361 ctttaactgt atttctgatt actcgctcaa tctgcatggc acttatctgc ctatcaacca
    27421 ggaaagacaa atctcgcctt atttgtggat atttggaaat aggcttatac agaggaatag
    27481 tcggattgat taatgattca agattcaatt caaacaaaac cacatcctgg tcaagatcaa
    27541 aggcatccga caagcgagga tgcagcacac cgatccagcc tgaatgcttg ccgttaatca
    27601 caatctgagc tgactggccg gggtgaagcg catgatgaga tgattgaata aattcaacat
    27661 catccagctt caatgaagca aacaaggatt gtaaatcccc ttttaaatca taaaaatcaa
    27721 ataaacgggc agattcactc caattcaaat tgccttgctc ccccattaac aagccagcaa
    27781 tacatgaacg ctcctttaat tgccctccgt ccaaatcaaa aaccacgcct atttcaaaga
    27841 atttcactgc ggtttgttgt ctgtgactat tataaatcat agatgcaatt aagcctggcc
    27901 acattcccgc acgcatttgc gataattcag aggaaattgg attaagcagt tccataaact
    27961 ctttttgtgg ataaagcgct tcctgcaact caggatcgac aaaactataa ctgatggttt
    28021 catggtagcc tttggcacta aaccacgatg aaacgtgtgt cgcaattttt tccttggcac
    28081 tgattaaacc agcttgtaat gatgtttgca ttggctgagc ttgtaacttg tcatagccat
    28141 ataaacgaat aatttcttca accaaatcag catcttgttg caaatcgact cgatgagagg
    28201 gaatggtgac ctcaaaaaaa tgattggttt ctttggtgat gacaacacct aaaccttcga
    28261 gcaaattttt catttcgttt agagggatgg acagccccgt taactttttc acttttgtcg
    28321 tatcaaataa aaaggaaacc gtacctggca aaaacttttt gtcaaaagac tctatcactg
    28381 gcccaggttc gcctcctgag atagataaaa tcaattccgt cgctctttca agcgcttttg
    28441 attgcaaaca aggatccact ccccgctcga atcgttgtga cgaatcactg aataagccat
    28501 attttctggc aactccggca atcgtcaccg gattaaaata ggcgctttcc aggaaaacat
    28561 gttgtgtctg ctcttgaact gcgctattag ctcctcccat tattccggcc atagccaaag
    28621 gcttttcttt atcagcgatt accaacacat tatcgtttaa tacaacttct tgaccatcca
    28681 ataattctaa ttgctcgcct gaagcgctat aacgtacatt aatttcacca ttaatttttg
    28741 ataaatcaaa agcatgcatg ggttgtccaa gctcgagcat gacataattc attacatcca
    28801 ctacagggtg caacgttcta atgctgcctc tacgtaagcg ctcagccatc cataatggcg
    28861 ttttcgcttc aagatttaaa ttacggataa ttcgaccgca atatcggggg catgcctcgg
    28921 aatgaattaa attcactcga agaccatcat cgatggctgg cggcactgta actattggtt
    28981 gctctattaa gggtaattta ttgagaacag ccacttcccg cgcgacaccc aaaatactaa
    29041 agcaatctgc gcgattcgga gtcaaatcaa tatcaaatac atggtcatca agggcaagat
    29101 actcgcgtaa atccattcca attggtgcct catcactcag ttccattatc ccctcggaat
    29161 gttctgccag tcccagttct gtcgcagaac acaacatgcc ctgagacaat tctccacgca
    29221 atttggattc cttaatttgc aaaccgccag gcaagtgtgc cccaatcatg gccagggcaa
    29281 ctctcaaacc aggcctcaca ttagccgcac cacaaacaat tttcaacggc ttgtctttat
    29341 taatattcac ttcacataag gttaatttat cagcatctgg atgaggcttg gtatttagga
    29401 cttcagcaac aatgacatga gtgaattggc cagctacagg attcactgcg tcgacttcta
    29461 agccagccat ggtcaattgc tcagccagtt cttgttcagt taatgagaag tttacccatt
    29521 ctcgtaacca taacttactt actttcattt aaagtcctca tctctgtaac tgttcatttg
    29581 gctcaaggtt tccatccgtt aattttgctc tctttatgat aatagagata aaattactga
    29641 taactatttg cctgtatcaa ctacgcaata aaatatcatt tccagtattc atggaaacta
    29701 tcccggatta caatttggtc taatctgtat tatttatgtg ccacatgtaa gacaatcaaa
    29761 agaaacataa aaataaaatc caggactcat tatttttata atggttaaaa ctgtcgtaaa
    29821 aaagttaagt cattttcaaa taacatacgc aaatcatcta tgccatagta aagcattgcc
    29881 aatctgtcca tacccatacc aaatgcccat ccatgatact cattcggatc aatgttcact
    29941 gcgattaaaa cgttagggtg gaccatacca caacccaaca cctcaagcca gcctgtaaac
    30001 ttgcaagagc ggcaaccctt accattacat tgagtacact ctatatcgac ttctgcagaa
    30061 ggttctgtaa aagggaaata ggaaggcctg aatcttaatg ccaactcccg accaaaaaaa
    30121 taagcgaaga aatcttgcaa caaacccttt aacccggcca aagtcgcttg cttatcaatg
    30181 agtaaccctt ccacctggtg aaacataggg gtatgtgtta catccgaatc acaacgataa
    30241 actcgaccag gagcaattaa acgaaaagga ggtttacgct gctccattgt cctgatttgt
    30301 acaggagagg tgtgtgtcct caacaagcgc ccgtctccaa aataaaacgt atcgtgcatc
    30361 gcacgtgcag gatgatggcc agggatattt aatgcttcaa aattataaaa ttctgtttca
    30421 atttcaggac cttcaacaat atcaaatcct aaacgactaa aataatcatt aattctatgc
    30481 tttacctgag taacaggatg tagggaacct gtggaatgat tgcgacctgg aagagtaacg
    30541 tcgatttgct ctgctgccag cttggctaat aattgctttt ctttcaactc gatcattttt
    30601 gtttcaatca aagcactaat tccctgcttt gcttgattga ccaattgacc taccttgggt
    30661 ttttcctccg cagacaaatt agcaagtcct tttagcaatt cggtgagctt gcctttcttg
    30721 cccaaaaaat caacacgaat ttgttcaaga gcaactatat ccgtggcttg tttgattgct
    30781 tctgaagctt gttcttgtat ggtgtttata agtaccaaca tagttctacc cacaagtaaa
    30841 aaggaggcct tagcctcctc aataattttt attttacaaa ccggctttta tttaagattg
    30901 tcattccgaa ttgagattta taaacgaaag gcaataacaa ataaagcggg ctgaaaatta
    30961 accagctaaa gctgctttgg cttgttctgc aatagcagca aaggcaactt tatcatgaac
    31021 cgccatatca gccagtattt tcctgtccag ctcaatggaa gcttttttca aaccatcaat
    31081 caagcggctg taggataacc cacattcacg agcttgagca ttgatacgag taatccataa
    31141 agcacggaat tgtcgctttt tctgccttct gtctcggtat gcgtattgac cagctttaat
    31201 tacagcctgt ttagcaactc gataggtacg gctacgggca ccataataac ctttggcctg
    31261 gtctaaaatt ttcttgtgac gcgcttttgc tgtcacacca cgttttactc ttggcattgt
    31321 tcaatctccc cgttaactac cgtgcaacat acgttcagcc aaacgcgcat cgcatggctt
    31381 caaagtacct gcttctacac gcaaatgacg cttttgctta gtagattttt tagtgaggat
    31441 atgatttctg taagcgccgc gacgtttaat cgctccactc gctgtctttc ggaagcgttt
    31501 agctgctcca cgatgtgact ttaacttcgg cataacaata tactccgcat tcgttacttg
    31561 gtttttttgg gtgataatac catcagtaat tgtcttcctt cacgttttgc atgctgctcc
    31621 actacagcta catccgccgt gtcggcttgc aaacgttcca aaatcttcat accgagttct
    31681 tgatgagcca tttcacgacc gcgaaaacgc aatgtgattt tgaccttatc accatgattg
    31741 aggaaacgga tcaggttgcg tagcttgacc tgataatctc catcttccgt cgtaggacgg
    31801 aattttaatt ctttgacctg aatttgtttt tgcttctttc ttgcttcagc ttgtttttta
    31861 ctcagttcaa agagaaactt accataatcc ataatacgac aaacaggggg tttggctgtt
    31921 ggagatattt ccaccaaatc caatccgctt tcttctgctg cccttaacgc ttctcgcgtt
    31981 gatacaattc ctgcctggtt accatcgaca tcaattaagc gtacctcagg tacagtgatc
    32041 tgttcattaa ttcgtgcgcg atcattttca cgcttagttg gtgcactaat ggtttaatcc
    32101 tccagttcgt ttatttatat tgatccttta cggataattt cttgcgttaa ggtatcacaa
    32161 attgtatcta ttgtcatcac acccaggtca atgccatctc gagtgcgaac agctacttga
    32221 caattttcaa cctctttatc gccaacaacc aataaataag gaattttttg taaagtatgc
    32281 tcgcgaattt taaatccaat cttttcattt ctcaagtcaa aattggcgcg aatccctctt
    32341 ttttgcaaag ttttcctcac tttttcggca tactcattct gcttctcact aatagtaagc
    32401 acaaccgctt gcaccgggga caaccataat ggcaatttac ctgcataatg ctcgattaat
    32461 attcccataa atcgttcgag agaacccaaa atggctctat gcaacatcac cggtgtttgc
    32521 ttgcttccgt cttcagcaac atagcttgcc tcaaggcgtg cgggcataga gaaatccact
    32581 tgaatcgttc cgcactgcca aattctacct aaacaatcag agagagagca ttcaattttg
    32641 ggtccataaa atgctccttc tccaggagca tccacccact caatatttct tccctgcata
    32701 gccagtttaa gggcggtttc cgccttatcc caaacgtcat ctgaaccgac tcgtttttca
    32761 gggcgcaaag ccaatcggta ttttatttca gtaaacccaa agtccttata tacggattgc
    32821 actaactcca gcatcatagc cacttcagat tgaatctgat cctcagtgca aaaaatatgg
    32881 gcatcatcct gcaccatatt gcgtacccgc atcaacccat gcagcgcgcc tgatggctca
    32941 cagcgatgac aattcccaaa ctcagatagt ctgagaggaa gatccctgta acttttcaaa
    33001 ccatgattat aaatttgaac gtggcaggga cagctcatag gctttactgc ataatgtcga
    33061 ttctctgttt cggtaacaaa catttcatct ctaaagttcg cccaatgacc tgatttctcc
    33121 cataacgatc tgtctactaa ttgcggtgtt ctaatttcct gatagccaaa atcgaccagg
    33181 cgatttcgca tgtaatgctc tagctcttga taaatagtcc atcctttggg gtgccaaaaa
    33241 accattcccg gagcaatatc ctggaaatga aacaaatcca gtgccttgcc taatttacga
    33301 tgatcacgtt tttctgcttc ttccaaacga aacaaatatt cttccaggga ttttttatca
    33361 gcccaggcag tgccataaat tctctgcaac atttcgttat ttgagtcacc acgccaataa
    33421 gccccagcta cttttgtcaa tttaaatgct tttaaaaacc cagtggaagg gacatgtggg
    33481 cctcgacaca aatcttcaaa atcaccttgc ctatagagag agagtgcttc attttcgggt
    33541 atatcagcaa taatttttgc cttatattct tcgcctaaac ccttaaaata ttgtattgct
    33601 tcatttctag gtaattctcg acgagtgatt gaaagattag cctttgccag ctcatgcatc
    33661 ttcgcttcga ttaaactcaa atcatcagga gtaaacgagc gctcaaaagc aaaatcataa
    33721 taaaacccgt cttctattac aggacctata gtcacttgag ctgatggaaa taaggctttt
    33781 acagcctggg ctaataaatg agcggtagaa tgcctgatta tttcaagact gtcttcatgt
    33841 ttctcggtta ctataatcaa agagcagtct tcttttataa gataggatgt atccaccaag
    33901 acgccatcga cacggcctgc tatcgctgct ttagctaatc cagggctaat gtgatgtgcc
    33961 acatcataaa tagtgagtgg cgcttcaaaa tgttttacat ttccatctgg taatttaaca
    34021 tttggcataa tcttatccgt ctttccatct ttcaggttct tgttaaaaaa aaaccctgca
    34081 tggcagggtt caaaccttta ataattcagt gcagtagttt accaattaga gattctacta
    34141 gaaatccagg aattatggta ggcacgagtg ggatcgaacc accgaccacc accatgtcaa
    34201 ggtggtgctc taccactgag ctacgtgcct ataattcaac ctgaatgaaa attggcatga
    34261 attatacaca taagattcat ggaaatgcaa tcatccaaat catatcattt ttatctaatg
    34321 aaaaagcaag ataacatgac attaaaattt tggatgagct tggtctatca atcttgcctt
    34381 ttaactcttt tgcaaatgca acccttctca accaaaccgc aaaacactct tgctttcccc
    34441 caaatgcatt agtgcaagaa gaataaagag catgaaaagc taattccaac tctcttggga
    34501 acaaggtaaa atgataaagc ctccgttagc atttaaaata ttatgtggtt taaaatcata
    34561 catccccatg tagaaatagc tatttatgcc aagccgaacg caaagaagac aaaactcatg
    34621 gccatttctg atgataggct gcatattgcg ctccatgcca aaccgcagga aggagaagcg
    34681 aataatgagc ttttattctt tatatcccaa tttttcaaaa ttccaaaaac acaaatcgaa
    34741 ctaattaaag gaaaaagcag ccgtcataag ctaataagat tacccttatc agaatctgtg
    34801 ttctgctttc taaataatcc aaaaatctag aacatatcac cctttaaaac ttagaaatgg
    34861 cccgaatgaa ggggttcatt caagccaagc aatgcgaggt taaccgctat ggttaaacca
    34921 ctttttctgt tgattggact accctgtgat acttgttcct tcaagtaaca tcagcgtccg
    34981 atatttggac ttaaagaaac gtgttataac gttccaatac caatta
//
LOCUS       DASLOE010000018        32869 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.018, whole genome shotgun sequence.
ACCESSION   DASLOE010000018 DASLOE010000000
VERSION     DASLOE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 32869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32869
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.018"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            96..1388
                     /gene="dctA"
                     /locus_tag="U6309_000679"
     CDS             96..1388
                     /gene="dctA"
                     /locus_tag="U6309_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HEN5504133.1"
                     /translation="MNFLKKLYVQVLLGVFIGILLGYFMPDVGVQLKAIADVFIKIIK
                     MLISPIIFLTLVSGIAAMNDMRQVGRLAGGALLFFTFITTLALLIGLLATDYFKPGVG
                     LNVNPNSLDAKEASSYLEGAHKVTNVTDLLINIIPRTFISAFVDGELLQVIFISILFA
                     IGLILTGNVGKSLVTGMQVLSNVFFKIIHFVMYMAPVAAFAAMAFSVGKFGVGPLFNL
                     IGLLACYYLTSIFFILIILGSLLQWYCKINIWHLLRYLKDELLIVWSTASSETVLPSL
                     MQKLERLGCEQSVVGLILPLGYSFNLAGTAIYLTMAAMFIAQATNTDLTVWQELSLLG
                     VMIISSKGAAGVSGSGFIVLASSLATIGHIPAAGVVLVLGIDKFMNEARSIINMIANA
                     IATIIISRWQQSLDYQKTQFELQEKGQTETKNVYASGM"
     gene            complement(1548..4448)
                     /locus_tag="U6309_000680"
     CDS             complement(1548..4448)
                     /locus_tag="U6309_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504134.1"
                     /translation="MLELNDKETAVIKAIHHNHKKRLESQMITRFGVYFEQSNHPEKL
                     YSWIEKIEDTRLLANHYLDTIALSRAPSTISSLEKETAGSSYYIEKAKLLIPESIRNF
                     LSDINSSNDNTLFNFVAAEVFSLALGKSITDLLFDYFNNEFLGAPENVQKEFNETLEK
                     QTELSNLIKKECERISIEIIKLFHFREMLLLGKISKSPIEPNSREEFIAKAKIANQSG
                     ESVHLAIEIYFARELSRIFNSGFKSLYQVHDVEIQSEHQSPLVKWINQMSESEQVRLA
                     FTQEIQLKFMLLARDFLLEKMNQKGILARYPIIFSLFAGVIIAPIVGVTMIIMTGGTF
                     LWAIAALAVLVFALSCIATHLLINKLSVLTYQRSSDNRAHIQQSIDMINGEFLRLKKE
                     SLLKKETTSEIIENTRDFQKINQNFHLVTIGKKVARGSVSGWLREYASRYRHSKAIEI
                     DLGDEYKELITQSRIQTQELIHSINKKNSKLLIKWIHDTMTYLQDEKNQSVIEEFELI
                     PKMKEQVLEVVCQLNYIPPMLLKFYSNSREKGGLGGNESDFSHIKRLTPIDAPDKPKH
                     NPYNYLCDTALNLFKKYEPLYTSTCIFLGDPEYRQMLGIARRDAGEPLTAENINRYLD
                     NSYAFLLSLCHKIKPGLGLDPLQQSARVTDEFVLYRMLLLKQLAAISESDSKEISNEI
                     KFKIRLFIQKYFNQDTKIIFDDLENQMFLLNKEAPNSRIYKNSDHFEVIDTELDNIFQ
                     AITLDMAYNSNNFRFIDILGYFINKFIKEHDSQSATVFAYGKAEQEINPQGTKYYLKT
                     LAQYCNNTTAFLKSAQTNTTLTATNLLDCYRYNISLQVCRTQIRIICSIKEINKLKIT
                     NETTEQITLLLKSFQQLAEFAKIHCFLLKTDSSCYRLFKLIENKAMQSTLSRDWIRQL
                     DAKEALVYMMDDLPNQLKLASDYNASSLFFSSAKRAKREKMENSEEPIMKPV"
     gene            complement(4754..5398)
                     /gene="upp"
                     /locus_tag="U6309_000681"
     CDS             complement(4754..5398)
                     /gene="upp"
                     /locus_tag="U6309_000681"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEN5504135.1"
                     /translation="MDFNQVKVINHPLIQHKLTIMRKKETSTVKFRTLMHEVSMLLAY
                     EVTRDLEIEYEEIETPLATMQSPVLKGKKLVFVSILRAGNGLLDGMLQLVPTARIGHI
                     GLYRDPKTLEAVEYYFKLPEHTQDRDVIVVDPMLATGNSAIAAVKEVKALHPKSIKFL
                     CLLASPEGISNFHGEHPDVPIFTAAIDEQLNDHGYIVPGLGDAGDRLYGTKLAH"
     gene            complement(5411..6652)
                     /locus_tag="U6309_000682"
     CDS             complement(5411..6652)
                     /locus_tag="U6309_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="URC4/urg3 family protein"
                     /protein_id="HEN5504136.1"
                     /translation="MNNQEQQIQNVLNTLCDPRTIRTQSHRILNLAKQNRLEHFSLSP
                     ERMTTTASYIVDIITSRYPEFDIPYHSRWRHFEMDGLPRINNLRKQLEPISPSEWGKI
                     LYELVIISVFLDAGAGPLWRYKEALTKKEYSRSEGLALASLYLYESGAFSADPTNPFR
                     VDAERLLDFKEAELIKGFQVSSTNLLEGIPGRVSLLNRLGEIIHRKEHCFNRRRRLGE
                     FYTYVESLQDNKILPTTKLFEAVLAAFNDIWPVRLIYHGVSLGDVWIHSALKTNEAGS
                     EYIPFHKLSQWLTYSLVEPLEQTGIDVTDLDVLTGLPEYRNGGLLIDTELLKVRNNKI
                     LEQPQDPGSEPIVEWRALTVALLDELALLIRQQLNMNAESLPLAKILQGGTWEAGRQI
                     AQKKRINGTPPIEIISDGTIF"
     gene            complement(6645..7913)
                     /locus_tag="U6309_000683"
     CDS             complement(6645..7913)
                     /locus_tag="U6309_000683"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEN5504137.1"
                     /translation="MSPDDSKKKSKGHIVLSSHPASHTQAPLKIKWGEKNPKERGPVI
                     ASLTNIKNRNAVGTHSGSYSVYRALAVAAGLLDPEHVPDLTDTLPPVDIGPHRQWGEA
                     GKIVSLDPWGHLVASVFSEEIKAGYDIRPTIAVTKAHINIPELHIAMQKGRLKPDGKI
                     LSETGDVVVTKAAIEPVWYLPGIAERFQVSEALLRRTLFEQTAGMFPELVTRSDLDVF
                     LPPIGGLTAYFFGDVTTIHNPEIELTCRIHDECNGSDVFGSDICTCRPYLVHGIELSI
                     ESAQKGGAGLIVYNRKEGRALGEVTKFLVYNARKRQKGGDTAAKYFERTECVAGVQDM
                     RFQELMSDILHWLGITKIHRFVSMSNMKYDALLKSGINVIERVKIPDELIPPDARVEI
                     DAKRAAGYYSPEGIIDINELAKPKGRALHE"
     gene            complement(8000..9208)
                     /locus_tag="U6309_000684"
     CDS             complement(8000..9208)
                     /locus_tag="U6309_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEN5504138.1"
                     /translation="MSDISRGDIRVKGFTDSEMDFQLIRQLGSSSYKAASVGECLNIA
                     NTMKNEDPEEWVRQFEQLAEWQKKDGLERMVKNHVISGREQLFKASNSFRAAEYYSPP
                     SSEHHRKLGLNSADCFCTAISSMDIHFENHSIPYKNINLPCYFISPANDGEKRDTVMI
                     VSGFDGTLEEEFLMRGLAAVERGYNVIHFAGPGQMDVFRKYPDTCFEPDFEQVVKRII
                     NYFEFRQEINMAKLTLMGISFGGYFATRAASHEPRIKVLIANSPILDLHAYMCSFVKM
                     DPCEMPDSEDFSINDLPSIPNEVMPPQLKAQTEHLIKRFGQPTFKATFNYLREFTVGN
                     SLNHIKIPCLALLGSGEGGEPGKQFAEFCKITNSAQYEFSDFEGAGSHCQVGNVSYSN
                     AVVYDWLDSL"
     gene            complement(9436..13293)
                     /locus_tag="U6309_000685"
     CDS             complement(9436..13293)
                     /locus_tag="U6309_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2239 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504139.1"
                     /translation="MKMNEYIELYERHRYEGNNTIDSLILTLNSLKQSRESWGQDNKL
                     AKYLGFGLRNAKHPFFQPKVTQKNLHVIETVIEKLNALKESPDYQQEGETSTTRLDIL
                     GILGERFNELSKNGKTRELFNYVMFNEGSIDFSMRESTLVEPSPLEILTGNEEPYEDE
                     QTWEPPYIHQLLDSKGIPYTIFDSLETNDLNLENDLGDHVRWAYDNIYRYSYQDSHQE
                     STIARFHHGIQHVTRAANYACAFANLYRRHGNEEAQNLTETDIKLIQIALLFHDSARE
                     GDDEDLWDHESAIFLYYYLTRVLHIDAETARFVAEATANKDPSPENGYFQLIEDEYGQ
                     LFWQFAKYEEGDFPAKNIYQKIIHDCDCLDIIRARNQYDATYLDFYKDIASKDNVALE
                     EMAELVVEARGLIYSQGDSYITKKAEIKAGYENEEAHTRIIRDMQSEAYPILSTLYGK
                     LLSIEELKAMTLVNLTPFDETAGMAGENLAAALREGRVLARGITTPSAMPTPRPNKPT
                     LTDETLAEREMRKSMRSDNINIKSRKEAPNLKYKNPLRSTSILGYGSGVYPAAGMLIF
                     NPDIGQIKRISAEDFDSGRGQKNHLAHLQEKEYKESREILERELDDLHHKMKLGLFGK
                     KTQLGSNYVELLYDINKYDAIFFTNDPTIGNEIAYNDFNPAHPLSPLLQAIYLQKQYA
                     RQYEETRKAYYERYGQERGETLFLERFGPNQFLPIYEYSGLRNKLTPYDTEKLTDENI
                     VNMWVEMSSDFMRNALSHMGRDNSYDFYDELLYNMSIEDIKVLSMYKTKGNILAKVNQ
                     PADLSYNVELREKITDAIAKAKEKIIAEYEEAVFKRLQDNQLSPFSKEAFLALEYSPR
                     LRELCSDKIKDDLLRFIYSPDGVISYKLSDIAPTSLKEKAITLDIQYCSTKERDFLCK
                     NEFLKAFILCQQLGLEGEIQFMRNKVNELAKQAIENTNAFIPENIYDLQSRLYQMINL
                     HNIMTLMDKDNHLTDNIESMLLEIVSKLTATYTDERLDLYNSYMLLKTLQNAGIPDVK
                     LKHYFRPVLNKANETLHERKPHDWGHYCSIAKLVGDEAKPYIYSWLNDYDKPFYSEIN
                     SMLETFKNAGIVFDDENLDVFKSLLEKIHYNDVAYNYVRLSYWLRVMNNLQSLAPENH
                     YSESQLEILKNQFDRVIEKLIKTTKSEYEHASYYSQEQKCYRQLTSFAEEINTILASD
                     FCLPVPDSLITEFNLTLIAFSEMDLSQTKVDKSKFHLLKQLHNKLPNPEKREHAMEKL
                     TESMDTKKQVEKDQNQNISAAGL"
     gene            13505..13891
                     /locus_tag="U6309_000686"
     CDS             13505..13891
                     /locus_tag="U6309_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEN5504140.1"
                     /translation="MYTVIICLFIAILLPYLAKVPVAYAMHKAGGYDNRYPREQQARL
                     QGFGARALAAHQNAFESLLIFATASLTALATNSVTLMTQYFAIAYIISRLFYHLFYLL
                     NWSSLRSTVWFLGVICCLSILWLSIP"
     gene            14054..15886
                     /locus_tag="U6309_000687"
     CDS             14054..15886
                     /locus_tag="U6309_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN5504141.1"
                     /translation="MSDLQQLPKTILRFSWHFIKKSPWLYALFFIAPVVMVLETNVIP
                     YALKMIIDGITNHKGGKESIFQDIAPALYLGGVAWFGVILVLRLHNWWQAYLVPQFQA
                     NIRMTLFGYLIQHSHHFFSNQLAGNLANKISDIPRSLEAIRKIVSCNVNTTFAAIIVS
                     IVLMLTINPWFAFILLSWIIIQLFISFYSAKEINKLARVNAEDKSILKGKIVDSLNNI
                     NTVKLFVQNEYEKKYVWKTQDKEVKSNKRLVLFINFFRLLVDVPVSIMLIAMIYSVIN
                     FWQRNLISTGDVVFIFATSFAIMNQMWSLSNSLCDLFVEIGIVKQALAILSQPIEVQD
                     IANAKELKVTHGEIRFENVTFRHRDGQELFTNKSLVIPAKQKVGLVGYSGGGKTTFIN
                     LILRYFDVDSGRILIDNQDISEITQYSLRKSISMIPQDTNLFHRTLLENIQYGKEDAD
                     RDEVVTAASLANCMDFIQNLPQGFNTIVGERGTKLSGGQRQRIAIARAFLHHTPILIL
                     DEATSQLDSLTEECIQESLQPLCEGKTTIVVAHRLSTLLNMDRILVFDKGTIVEDGTH
                     QELIENNGLYKSMWDAQTSGFLPVEQEDEAELAKEFDRPYLAQV"
     gene            complement(16079..18421)
                     /locus_tag="U6309_000688"
     CDS             complement(16079..18421)
                     /locus_tag="U6309_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEN5504142.1"
                     /translation="MHTIANETPVQKDHSFDPITNILKWVLLVTAILCFLILIWGTVK
                     TYRLVPPLPDRFVTSTGETVMTSQDIVEGKAGFQRADLMDYGSLYGMGSYFGEDYTAK
                     YLVRLGELVQEELAKIRFNKAYSELTESEQFVINKEMQDSLQRLDLNKKTSILTPAIA
                     TSIRQLQTEISQSLLNHNFMAGYTQAYSLNPDTALQTANFLIYSSLTTIARRPNQNIS
                     YTNNWPYEPSVGNTPSSGTFYWTWISFCFVFMGFGAVIYIYHRYLSDVDHGPMEPIFS
                     GFKPLTQSQRKVGQYFLVAALFLLLQIAVGAIMAHYYAERESFYGINVNTWLPFNFLR
                     DVHIQTPIIWIGLSWISAGIFMAPIISGKEAKGQGFLVDLLFYVTLLVVGGAILGNYL
                     GIMGYIDKAWFWLGNQGLSYIQLGRLWQIGFCIGLFLWSFIVFRGMWPTWDGLKKATI
                     EFWTGRIKLENLFWASTINVAVLYCFGMIPLTGIEKSFTITDFWRWWVVHLWVEQSFE
                     FFAACATAYLLMGTGLVSRKAAERTVYFESILIFLGGVIGTGHHWYWTGTPEMWIPLG
                     SMFSFIEVLPLVLLIIDAIEHRQLIKKQGTFTYNLAYLYILGAAFWNFVGAGVFGGGT
                     LNAPLINYYEHGTFLTLNHAHTALFGAFGLLALGLIYFCLRYAAGESKVWSDRLGVYA
                     FWLYNAGLFLWIVLNFFPIGWSQLMAVYEHGLSYARSLEFYNTTLLWQWLRFPGDVVF
                     ALGALLMAYDFIVKIGPFFPKFARNRRFIAGPPKATDPSL"
     gene            complement(18574..19776)
                     /locus_tag="U6309_000689"
     CDS             complement(18574..19776)
                     /locus_tag="U6309_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEN5504143.1"
                     /translation="MDINLMVFASPLFLILIGIETWVAYYKKSGVYQLNDSVNNFSSG
                     ILEEIGALPIKGLIIYSYYYLYEHYAIFNVNTQSIFSWLLLWIGVDFFYYWYHRVSHR
                     NSFFWIGHSVHHQSEHFNLSVALRQGYFQTLTSWVFYLPLALIGFPTWMFVIVASVNT
                     IYQFWIHTESIRKMGWFEKIFNTPSHHRVHHGKNPQYIDKNYAGSLIIWDKLFGTFEP
                     EEAPADYGVTEPLDSWNPFYANIKVVKDILYYGKGLKSKWDVLRAFFMPPEWIIHRLE
                     QENSPVSKKTAIKKNTTSPGIYMLTNTAFAVFLYTYLSLVFDPSMVTSWLMGALILLT
                     LYTLGRIADGHSNTLYLEISRATLILLILLLFKNTLLLSLGCSVLFYVINYALCTTSP
                     YKRTASSF"
     gene            19930..21153
                     /locus_tag="U6309_000690"
     CDS             19930..21153
                     /locus_tag="U6309_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEN5504144.1"
                     /translation="MHQEHLGQTDTPKIDPWLIFVILMLMALMQTTTDQYIPSLPAIT
                     TALNSTEASIQLTISMFMLGLSLSHIFYGPLSDKIGRKPPLMFGVGLSILGSLVCFFA
                     PNVSVLILGRFLQGFGIGCCNSVGRSLVRDLFTDRLLAKIGSYVGVVSIFIMAASPTL
                     GGFIQEMAGWKANFMFLFVFGILVWLLAFCILPETNKHLNPEATKIKVMAKNYFTLLR
                     SKIFVGYTLCACFAFAGMVSYLTIAPFLFQNTMGLSPLEFGRLTFFIAGAICFSGIVN
                     SQLVMRKGISFMVCTGVLFMIVGGLSLLLITILGWSQIYFIMISMTLFSAGAGFTFIN
                     AFAGAFHPFPQMAGTVGALYASMQDLSAALTSGFIAGIKGYGQYSLAMILLILGLSSL
                     VAWYYLGLQDDAISN"
     gene            21163..21390
                     /locus_tag="U6309_000691"
     CDS             21163..21390
                     /locus_tag="U6309_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN5504145.1"
                     /translation="MKITDEELKKIISKLSGSKADDIQDDTALIEDLHLDSLKIVELL
                     AILSEEYQLEVSEEDAMNFHTYKDLYDFTQA"
     gene            complement(21710..22720)
                     /locus_tag="U6309_000692"
     CDS             complement(21710..22720)
                     /locus_tag="U6309_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEN5504146.1"
                     /translation="MNFFRCEKPIYIKGPCVALPDRIMSNQDVLNWMDSTQNPAVIGF
                     STGIKNRHWVNEDQACSDLAVQAAEQLFMEKPEEKHKVNQVILATISGDYPSPPSSPL
                     VQYRLGLQNAGAFDIGAACAGFVVGLHTSAALAQTNGGSVLLIASEIRSKFLNKNNFA
                     TSVLFGDGAAACCVSQDKEKADFRFIASALFADGEVYDAVSTPAGGSRLPAAVCNDNE
                     QFYITIKESTALFVKAVHGMADSAKDFLKELNLTISDIQWLVPHQGNKNLVLSVAKQL
                     GFPEEKTIKTVEETGNTSGSSVGIALDRLRSDGKIKSGEKVLLVAAGGGGIAACSLLE
                     VI"
     gene            complement(22717..23469)
                     /locus_tag="U6309_000693"
     CDS             complement(22717..23469)
                     /locus_tag="U6309_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN5504147.1"
                     /translation="MNILITGGSSDIAQAIAKRRSESGDKIIITCSNDDSLHETLAIY
                     RQQNIDVTGFIYNFSDPQACENDLELILREPLDGIILNAFTRVTQLRKLHALPYHAVR
                     TYLDNNLNGNIWLIHRLLPALLKNKFGRLVLISSLSATAGTSRYPVYCAAKAALEGLF
                     LNLAVDYSANNILSNIVRVGLIKTSRTEQFWKNPAYQEKVMQIIPQGTMGEPTQVAEA
                     IDPLLSKTSFMTGSVVTVSGGLPLMRSEGLLS"
     gene            complement(23466..23699)
                     /locus_tag="U6309_000694"
     CDS             complement(23466..23699)
                     /locus_tag="U6309_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN5504148.1"
                     /translation="MLSNRIEQVLERIGLTNLPENKQAKLEQGGLDSLMLALLIIELE
                     REFEIKIPVIPLEKGHYESINTIEQYLIELGAK"
     gene            complement(23689..25068)
                     /locus_tag="U6309_000695"
     CDS             complement(23689..25068)
                     /locus_tag="U6309_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HEN5504149.1"
                     /translation="MLFLAPDLPNKVAITQGNRAITFHELTHEVLASSKQFLKLPKTI
                     IVLHARHEIGFIIQLLACLETGRPIALFPNSISEEEKQRRLSLLGNAVMINEEGELQE
                     ICENKTIQPHPQTALILFTSGSTGSVKAVQLSSINIKANCHAVIKALEFKKIQDQLLF
                     LPLSYSFGLLGQLLPGLMFGLTTRLITHFTDIKMLMEQSTVPQMWSGVPSHWVAINAI
                     AKRYPDSAAKINAIVSAGAPLSTALRADLKQIFPNAIIYNNYGLTEASPRVLTYSSKD
                     PLFTENYAGYPVGDWKVKLSAEHELLISGTQMMLGYLGEKDSTKIHNGWLSTGDIAEI
                     LPNGLVAIKGRRDSLVNIGGEKVNLSEIEQKICQIDVIKEAIVIPQEDKIYGTRLIVC
                     IEKKSLPSSISEQNLIEKIQTHLLPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIDGL
                     DNKEKNNAK"
     gene            complement(25073..26818)
                     /locus_tag="U6309_000696"
     CDS             complement(25073..26818)
                     /locus_tag="U6309_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEN5504150.1"
                     /translation="MKKEYLQCQSLVDVVRLRALHSPNKKSCTFLNKELEETMTYEQL
                     DQHAKAIAATLQAEGAKPGDRVLLLFAPGLPLIQAFLGCLYAGCIAVPIYPPAQEKLL
                     DKAQRIVTNSKPVIVLMIADHIKKFTADELNTNPKFLKIPAIALDSIELNRSSSWQPT
                     SIQSNDIAFLQYTSGSTMHPKGVMVSHHNLLDNLNKIFTSFHMNDETIIFSWLPPHHD
                     MGLIGCILTPIYGGIQAIMMSPFSFLQNPLSWLKHITKYRATISGSPNFAYDYCVKRI
                     REEKKEGLDLSSWVTAFNGAEPVRAETMEHFYQAFKEFGFRKEAFYPCYGLAEATLLV
                     TGGTPGSPYKTLTLAKEQFQDHRVHFADDNSPGSYKLVSSGNPIQAVKIIDPDTLIPC
                     DFDQVGEIWVQSNSVAKGYWNQPEETKHAFAGKIKDDERSAIYLRTGDLGFLHENELY
                     VTGRIKDLIIIYGKNHYPQDIEFSLMHSPLHHVLGKCAAFVIQEEHEYKLTVMCEVKN
                     RFMDDVAQDNLFNEIFELVYENHQLEVHTIVLIPLKAMPHTTSGKIRRNFCRKHLLDK
                     TLPIVATWQLNKIEE"
     gene            complement(26830..27891)
                     /locus_tag="U6309_000697"
     CDS             complement(26830..27891)
                     /locus_tag="U6309_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEN5504151.1"
                     /translation="MNILKPNIQFEITGASRILPASGPITNLEILKNFPRTADKSQGF
                     LEKFAEKIGEEFGFHTRYWCHKPWESLDNSRELSSESLAMQAVDKLITQSRSKDVDAF
                     LLGSTTNKRFTGSQAAAVLGNLKLNAPAYDLKTGCSTSLSTLHFAYAMMALGYQKILV
                     CCSETLSKVIDPENEKTWIGLADGAASLLLEKSKEGSFTIEKSFFSTDGQYVNAFTTQ
                     GVFPPTHEQIDSVGYHLVGDETLMKELAYSRYMQMLDNLLPTEKEKNEITWVIPHQVN
                     RKLIDQVLHESHLNNKTIIWDADTIGNIGGASVLYTLARAVEEKLFDRSGKILLMSVG
                     GGLSYAGQVLNYQKASYSN"
     gene            28118..29539
                     /locus_tag="U6309_000698"
     CDS             28118..29539
                     /locus_tag="U6309_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl CoA carboxylase subunit alpha"
                     /protein_id="HEN5504152.1"
                     /translation="MSSQHTDKTLIKWIDNLIDEQSFLPIGSEYIPDNTPHSLPGAEV
                     ITGLAKVNQRPVAIYAHDSSVNRGYVTRQGARKIIHLMDKAEKLRIPIVAFLASPGVS
                     LEENLLSGDEYTQIIARNIRLSGIIPQFAVILAPTLGAPAYSAVLMDLLFFNKHRSYL
                     MVTSPTVVQQAIGEKVTMGELGSATLHATTTGIADFVDDSVTAQINHVKAILGFFPLH
                     TNERPPVHPIMEPNHPIPDIPANPRMPFNMLNLIQAVVDNSNVIQYKRTYGQAMICAF
                     VHIHGFAVGVVANQSIRYSGAIDSDAAQKAAKFIRICDAYSIPILTFIDVPGFMPGKR
                     EEQKGLLQHGARFCVAMQTRVPRLSVIVRKCYGAAAFLMMQTKSQNGDLVLALETANL
                     GVMGKETTNKVQAMDGQPVQESSQSQLTEKSQIDDSLMEAYSLGLIDEIIQAAQIRSR
                     LASHLEFLYRRMETMPPAKHSIV"
     gene            29548..31239
                     /locus_tag="U6309_000699"
     CDS             29548..31239
                     /locus_tag="U6309_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEN5504153.1"
                     /translation="MTSYENLIQLSEFFESCLGNPLEDNSPVNFQQILLADEQEILAL
                     SAIEFIKQWGYLDYTVPHYLGGKLSSLDELYTLTRLLARRDITMAIMFGLAFFTALPI
                     WIAGNSKQKKHLSESLRQGKIGALALTEEEHGTDLTSNKMNAKPTEDGWEISGRKWCV
                     NFATLSEYAVVLCRTNEKGGLLGFSLFYIDKKLAEKRISPTPKLPTHGVRGLDISGFS
                     FDKVKVSKDALIGKEQRGLEITYKVFQISRTLCACLAIGGADTALRLALSYSLQRQLY
                     GKSVFEIPAVKQRLGELFTLLLIADCTAQVMVRACTVIPEKMSLWSAIIKFLIPHIGE
                     DIAEQCAIILGARAYLRTTQWAIFQKIRRDIQVVGLFDGSTQVNLSIIAGNLLPQAGM
                     RGKHRAEHSEKIEKIFNVRLSCPPFKEDRLGLFTHEEDDVLAGILSLNSIPINPLITT
                     IRHEIEKLDQEVIRLHDEKLFDPRSLAVFRLAEKYCWIFAASCCLQFWHYNQEILSDD
                     LQNTDWLDLAMQLILKRLNSGSMIDSVLQEAMSERLYSFYQEKQLFSIMPIHIAG"
     gene            31246..32775
                     /locus_tag="U6309_000700"
     CDS             31246..32775
                     /locus_tag="U6309_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEN5504154.1"
                     /translation="MNWKRFLIAAMTKKKYQVFLNDTKHPKEARERLWNSEIVPLLKK
                     SSYWQKLLKDQSSVALNDFGITEYEDYQEGLLAAQHSTIQPFNGEELIFWSETSGTSG
                     VRKFFPITDSFQKQFQRTMAPYIYSLTQRFPGFFKEKMLYLVAVDTHKTTPAGIPTGW
                     ISNFNYRHLPSFIKKFYAMPDEVFASHEAYNQWGPIYGLASDLSAIFAVTPMVIDAFY
                     QRCIEGFKEYLPYLLGNKPLPSHLPPLNMTRKRRRHLQALAKVDHLSFKQLWPSLEMA
                     GCWTSGLCEYPAQQLQKLLGSEIKLVDGTYSATEGWLTVPVETNSVGGILHPGAHIVE
                     FIPEGEAIEKENLLQCWELEVGKKYEVFLTTAMGFIRYRLKDIVKCTGYLNSSPKLEF
                     CYKTQLLKLETCSITGRELQSAVLATPLNIEPYWYFARNSVGNRIVLVTDDEVEISEL
                     SLKKLHEQLIHISPTYAHSVETEEVLPVTLLQLPKEKLLAGMHGQTKPKFISQEVIAE
                     R"
BASE COUNT         9468 a         6226 c         6632 g        10543 t
ORIGIN      
        1 ttcatgtaat aatgatttgt ctagggaaag aatttttagt gcttcatgct ttgcttttga
       61 atagttgttt atagtaaacg acctggagaa taaaaatgaa ttttcttaaa aaattatatg
      121 tacaagttct tctgggtgtt ttcattggta ttttacttgg atattttatg cctgatgtag
      181 gggttcaatt aaaagccata gccgatgttt ttatcaaaat cattaagatg ttaatttccc
      241 ctattatatt tctcactctg gtctccggaa tcgcagcaat gaatgatatg aggcaagttg
      301 ggcgcttggc aggtggggca ttgctttttt ttacgttcat cacaactctg gcattgctga
      361 taggtcttct ggcgacagat tattttaagc ctggtgttgg tttaaatgtg aatcccaatt
      421 ctctggatgc taaagaagcg agttcttacc ttgagggggc acataaggta accaatgtga
      481 ctgatctgct aattaatatt atccccagaa cttttattag cgcatttgtc gatggtgaac
      541 tgctgcaagt tatttttatt tcaatattgt ttgcgatcgg cctgattctt acaggcaatg
      601 taggtaaatc attggttact gggatgcagg tcctgtctaa tgtctttttt aaaatcattc
      661 attttgtaat gtatatggcg cctgtagctg cctttgctgc catggcattc agtgtcggga
      721 aatttggagt tggtcctttg tttaatttaa ttggattatt ggcttgttat tatctaactt
      781 ccattttctt tatcttgatt atcttgggaa gtcttttgca atggtactgc aaaattaata
      841 tttggcattt gttacggtat ttaaaggatg agcttttaat cgtatggagc accgcttcct
      901 ccgaaaccgt actgccaagt ttgatgcaga aactggagcg actggggtgt gagcaatctg
      961 tagtcggttt gatcctgcca ttaggatatt cttttaattt ggcaggaact gcaatctatt
     1021 taacaatggc tgctatgttt attgcacagg ctaccaatac tgatctaaca gtttggcagg
     1081 agttatcgct tctgggagtt atgatcatta gttccaaggg tgccgctggg gtgtccggaa
     1141 gcggatttat tgtgttggcg agctcattag caacgatagg acacattcct gctgcaggcg
     1201 tcgttcttgt tttgggtatc gataaattca tgaatgaagc caggtcaatt attaatatga
     1261 tcgctaatgc aatcgccacg attattattt ccaggtggca acaatctttg gattaccaga
     1321 aaactcaatt tgaattgcaa gaaaaaggac aaacagaaac caaaaatgtt tatgctagtg
     1381 ggatgtaaga tttctacgcg gtttttcata agccctgttc tgttgtgaga ggagcattta
     1441 acctgttaaa aaaacaggct tgcagtaaaa gtcttgatgt ccttttgttg gacatcaagg
     1501 ctctagtcta ttgtgttttt acttaccaat aatcccttca aattaattta gaccggcttc
     1561 attataggtt cttctgagtt ttccattttt tctcttttag ctcgttttgc cgaactaaaa
     1621 aataaactcg aagcgttgta atctgaagca agttttaatt gattaggcag atcatccatc
     1681 atgtaaacga gcgcttcttt tgcgtcaagc tgtcttatcc aatctctgga taaagtagat
     1741 tgcattgctt tgttttcaat cagtttaaat aatcgataac aagaagagtc agtctttaac
     1801 aagaaacaat ggatttttgc gaactctgca agttgttgaa aggatttgag tagaagggtt
     1861 atttgttcag tagtctcatt tgttattttt aatttattga tttcctttat gctacagata
     1921 atccgaattt gcgttctaca aacttgcaaa ctgatgttgt atcgatagca atcaaggaga
     1981 tttgtggccg ttaatgttgt atttgtttga gcgcttttta aaaatgcggt cgtattgtta
     2041 cagtattgag caagagtttt gagatagtat tttgtgcctt gagggttgat ttcctgttct
     2101 gcttttccat aagcaaatac tgttgcggac tgggagtcgt gttctttaat aaatttatta
     2161 atgaagtagc caagaatatc aatgaatctg aaattattgg agttataagc catatcaaga
     2221 gtgatggctt gaaaaatgtt atccaattcc gtatcaatta cctcaaaatg atcgctgttt
     2281 ttatagatac gggagttggg ggcttccttg tttagcaaaa acatttggtt ttcaagatca
     2341 tcaaaaatga ttttggtatc ctgattaaag tatttttgaa taaaaagtct aatcttaaat
     2401 ttgatttcat tgcttatttc tttgctatca gattcggaaa ttgctgccag ctgttttaac
     2461 aacagcattc tgtagagaac gaattcatca gtgacgcgcg ctgattgttg taagggatcc
     2521 aagcctaatc caggtttgat tttatggcaa agcgataata aaaaggcata ggagttatca
     2581 agataacgat tgatgttttc cgctgttaga ggctccccgg catctcttct ggcgatacct
     2641 aacatttgtc gatactctgg gtctcctaaa aaaatacaag tcgaagtata taaaggctca
     2701 tattttttaa acagattcag agctgtatcg cagagataat tataggggtt atgcttgggt
     2761 ttatctggcg catcgatggg ggtcaaacgt tttatatgag aaaaatcgga ttcattacct
     2821 cccaggccac ctttctctct ggagttagaa tagaatttca ataacatagg agggatgtaa
     2881 ttgagttgac agacaacttc aaggacttgc tctttcattt tggggattaa ttcaaattct
     2941 tcaatcacag attgattttt ttcatcctgc aaataagtca tggtatcgtg aatccattta
     3001 attaataact tggagttttt cttgttaatc gaatgaatga gttcttgggt ttgtatcctg
     3061 ctttgagtaa ttaattcttt gtattcatcc cccaaatcaa tttcgatggc tttactgtgc
     3121 ctataccgag aagcgtattc tcttaaccat ccagatactg agcctctggc taccttttta
     3181 ccaattgtga ctaaatgaaa attctggttg attttttgaa aatctcttgt attttcaata
     3241 atctcactgg ttgtttcttt ttttaataaa ctttcttttt tcaaacgtaa aaactctcca
     3301 tttatcatgt cgatagattg ctgaatatga gctctattat ccgaagaacg ttggtaagtt
     3361 aatacgctga gtttattgat caggaggtgc gttgctatgc aagagagagc aaaaaccagg
     3421 acagccagag cagctatcgc ccatagaaaa gtaccgccag tcataataat catagtcaca
     3481 ccgactattg gagcaataat gactcctgca aagagtgaaa aaatgatagg gtatcttgcg
     3541 agtatccctt tctgattcat tttttccagt aaaaaatctc ttgccagaag catgaatttc
     3601 aactggattt cctgagtaaa ggccaatcga acctgttcag actctgacat ttgattgatc
     3661 cacttgacca gaggcgattg atgttcactt tgaatttcca catcgtggac ttgataaagt
     3721 gatttaaaac ctgagttaaa aatacgactt aattcacgtg caaaatagat ttcgatagcc
     3781 aaatgaacag attcgccaga ttggttagca atctttgctt tggcaatgaa ttcctctctg
     3841 gaattgggtt cgattggtga tttgcttatt ttcccaagca gcaacatttc tctgaaatga
     3901 aataatttga taatttctat ggagatgcgc tcacattctt tttttatcaa attggataat
     3961 tcagtttgtt tttccagggt ttcattaaat tctttttgta cattctctgg cgcccctaaa
     4021 aattcattat tgaagtaatc gaataacaga tcggtaattg atttgcctaa agcgagagaa
     4081 aagacctcgg cggctacaaa attgaataag gtattgtcat tggaagaatt gatatcagaa
     4141 agaaaatttc ttatagattc cggaataagc agttttgctt tttcaatgta gtaggaagaa
     4201 cctgctgttt ccttttccaa tgaagatatt gtgcttggag ctcggcttag agcgatagta
     4261 tccagatagt gattagctaa caggcgcgtg tcttcaattt tttctatcca ggaatataat
     4321 ttttcaggat ggtttgattg ctcaaaatat accccaaaac gggttatcat ttggctttca
     4381 agtcgcttct tatggttatg atgaatagcc ttaatgactg cagtttcttt gtcgttcagt
     4441 tctaacattc ttcgctccct cgtagagtga ttagatatga ctttatgcaa taaggggtat
     4501 gccaagagac tatatttgct gttgcttgtt tcctggcatt tccctgccaa aattcttcag
     4561 aatcttgata catcacatct gttgtcccgc ataaacactc agagggagtc cacagaaaaa
     4621 ttggtggtaa tttttcagca tgcccttgtc aaaaagtaaa agcagtcatt actgaaaggg
     4681 caagacaatc ttaattatta tttaaaccta caattggctg attgggtatc cctgaaataa
     4741 ggataattca gcattaatga gccagttttg tgccatatag cctgtctcct gcgtctccta
     4801 aaccaggaac aatgtaccca tgatcattca gctgctcatc tatggctgcg gtgaatattg
     4861 ggacatcagg atgttctcca tgaaaattgg aaattccttc aggcgaggcc agtagacaaa
     4921 gaaatttaat tgacttggga tgtaatgcct tgacctcttt aaccgcggca atggctgaat
     4981 ttcctgtcgc cagcatgggg tcaacaacaa taacgtctct gtcttgtgtg tgttcaggta
     5041 gcttgaagta gtattcgact gcttccagcg ttttgggatc tcgatagaga cctatatgac
     5101 caattcgggc agtaggaact aattgtaaca tgccatccag tagaccatta cccgcgcgta
     5161 atatcgagac aaaaacaagt ttttttcctt tcaaaacagg tgattgcatg gtggccaaag
     5221 gagtttctat ttcttcgtat tctatttcca aatcccgagt gacttcatag gctaatagca
     5281 tactgacttc atgcatgagt gtccgaaatt ttactgtgct ggtttctttt ttcctcatta
     5341 tggtgagttt gtgctgaatg agaggatgat ttataacctt aacttgattg aagtccatta
     5401 ttattcctta ttaaaatatc gtgccatcac tgataatttc tattgggggt gttccattaa
     5461 ttcgtttttt ttgtgcaatc tgccgccccg cttcccaagt acctccttgc agtattttgg
     5521 ctaaaggaag cgattctgca ttcatgttga gctgttgcct gattaatagc gctaactcat
     5581 ccagcaaggc tacggttaaa gcacgccatt ctactatggg ctctgagcca gggtcttgag
     5641 gttgctccag aattttatta tttcttactt ttaatagttc ggtgtcgatt agtaaaccac
     5701 catttcgata ctcaggcaat ccggtcaata catccaaatc agtaacatca atacctgttt
     5761 gttccagtgg ttctactaaa gaataagtga gccattgcga taatttgtga aagggtatgt
     5821 attcagatcc ggcctcattg gttttcaatg cgctgtggat ccagacatcg cctaaagata
     5881 ctccatgata gatcaatcga acaggccata tgtcattgaa tgctgctaac acggcctcga
     5941 ataatttggt agtaggcagt attttgttgt cttgcaagga ttccacataa gtataaaact
     6001 caccaagacg tcttcggcga ttaaaacagt gttccttgcg atgaatgatt tctccaaggc
     6061 gattaagcaa ggatactcga ccgggtattc cttcaagcag attggtagag ctaacctgaa
     6121 aaccttttat gagctccgct tctttaaagt caagcagacg ctcggcatcc acgcgaaacg
     6181 ggttagttgg gtcagcgcta aatgctccac tttcatagag gtataaactg gccagggcta
     6241 atccttcaga gcgactgtac tcctttttag tgagagcttc tttatagcgc catagggggc
     6301 ctgcccctgc atcgaggaaa acgctgatga tgaccagttc gtagagtatt tttccccatt
     6361 ctgaggggga aattggctcc agttgtttgc gtaaattatt aatccgggga aggccatcca
     6421 tttcaaaatg acgccagcgg ctatgatatg gaatatcaaa ctcaggataa cggctggtga
     6481 taatgtctac gatatacgat gccgttgttg tcattcgttc agggctcagg gaaaagtgtt
     6541 ccaaccgatt ctgttttgcc aagtttaaaa tacgatgaga ttgagttcgt atggtgcgag
     6601 gatcacaaag cgtattcaaa acattttgta tttgttgctc ttgattattc atgaagggca
     6661 cgtcctttcg gtttagccaa ttcattgata tcaataattc cttcgggtga gtaatatcct
     6721 gctgcacgct tggcatctat ttctactcgg gcatctggtg gaatcaactc atccggtatt
     6781 tttacccgtt caataacatt gattccagat ttgagcaagg catcgtattt catattggac
     6841 attgaaacga agcgatgaat tttagtgatc cccagccaat gaagaatatc ggacattaat
     6901 tcctggaaac gcatatcctg aacaccggca acgcattctg ttctttcaaa gtacttggct
     6961 gcagtgtcac cacctttctg tctttttctg gcgttataaa ccagaaattt agtgacctcc
     7021 ccaagagctc ttccttcttt gcgattataa acgataaggc ctgcgcctcc tttttgtgcg
     7081 gactcaatgg ataattcgat tccatgaacc aaatatggtc tgcaagtgca tatgtcagaa
     7141 ccaaagacat cggagccatt acattcatca tggatacggc aagttaattc gatttccggg
     7201 ttatggatgg tagtcacatc accaaagaaa taagcagtta atcccccaat aggtggcaaa
     7261 aagacatcca agtcgctacg ggttaccagt tcagggaaca ttccagctgt ttgttcaaaa
     7321 agagtgcgtc tgagcaatgc ttctgaaacc tggaagcgct ctgcaattcc aggcaagtac
     7381 catactggtt caatcgcggc cttagtcacc acgacatccc ctgtttcact taaaattttt
     7441 ccatcaggtt ttaatctgcc tttttgcatg gcaatatgca attccgggat attaatatgt
     7501 gcctttgtga cggcaatggt tggtctgata tcatagccgg cttttatttc ctctgagaaa
     7561 acagaagcca ctagatgccc ccagggatcg agggagacaa ttttgcctgc ttcaccccat
     7621 tgcctatgag gccctatgtc aacaggagga agggtgtcgg ttagatcagg aacgtgctcg
     7681 ggatccagca gaccagctgc tactgccaag gccctgtaaa cagagtaaga ccctgagtga
     7741 gttccaactg catttcgatt tttgatattg gtgagagaag cgataacagg tcccctctct
     7801 ttagggtttt tttcacccca tttgattttt aatggagctt gtgtatggct tgccgggtgt
     7861 gaagacaaga caatgtgtcc ttttgatttt ttctttgaat catctggtga catgatactt
     7921 aattcctttt gtttcaacgg tgctcaaata aatcggttgt tttatctttt atccctttat
     7981 aagcaggtgc gcactgttgt tataaactgt ctaaccaatc ataaaccaca gcattggaat
     8041 aagaaacatt gcctacctga caatgacttc cagccccttc aaaatcacta aattcatatt
     8101 gtgcagaatt cgttattttg caaaactccg cgaattgttt tccgggctct cctccttcgc
     8161 cactacctaa taatgcaagg caagggattt taatatgatt taatgaatta cctacggtga
     8221 actctcttaa ataattaaat gttgccttaa aagtaggttg cccaaatctt ttgattaaat
     8281 gttctgtttg agctttgagt tgagggggca tcacttcatt gggtatagaa ggtaagtcat
     8341 tgatgctaaa atcttcagaa tcaggcattt cacaagggtc catttttaca aaggaacaca
     8401 tataggcatg taaatccaga ataggggagt tggcaattaa aaccttgatt cttggttcat
     8461 ggctcgcggc acgagttgcg aaatagccgc caaaactaat gcccatcaat gtcaatttgg
     8521 ccatattgat ctcttgcctg aactcaaaat aattaatgat tcttttaacc acttgctcaa
     8581 aatcaggctc aaaacaagtg tccggatatt ttcgaaagac atccatttgg ccggggcctg
     8641 cgaaatgaat cacattatat ccacgttcta ctgcagccaa tccacgcatg aggaactcct
     8701 cttccaaagt tccatcaaag ccactaacta tcatgacggt atctcgtttt tctccatcat
     8761 tggcagggga gataaaatag catgggagat tgatgttttt ataaggaatg gaatggtttt
     8821 caaaatggat atccatgctc gagatggctg tgcaaaagca atctgctgaa ttcaatccta
     8881 attttctgtg atgttctgag gaaggaggag agtaatattc agcggcacga aatgaattgg
     8941 aggctttgaa caattgctct cgaccgctta taacgtggtt ttttaccatg cgttccaagc
     9001 catccttttt ttgccactcg gctaactgtt caaactgtct gacccattct tcagggtctt
     9061 catttttcat ggtgtttgca atatttaaac attcgccaac tgatgctgct ttatagctgc
     9121 tggatcccag ttgtcgtatt aattgaaaat ccatttctga atcagtaaaa cccttcaccc
     9181 gaatatcgcc acgactgata tcactcattt tacttccccg ttatttccgt ttttacagag
     9241 tgttatgagc ttaatagtaa cggtacaagc caagaacatc aatcttaaat acaaaatcct
     9301 tgaatcatga taatgaataa aatatggtat aaattttata aaccaaaaga tgttaacatc
     9361 gcactatgaa ttgatttgct tttttaaacg cgcaaagggt acaaatcaga attgacacag
     9421 tctgatatta tcaggctaga gaccagctgc tgaaatgttt tgattttgat ctttttcaac
     9481 ttgttttttt gtatccatag attcagtgag cttttccatc gcatgctctc ttttttccgg
     9541 atttggcaat ttattatgaa gttgttttaa aagatgaaac tttgatttat ccactttagt
     9601 ttggcttaag tccatttcac taaaggcaat cagagtcaga ttgaattccg taatcagcga
     9661 gtcagggact ggtaaacaaa aatcagaggc caaaatggtg ttaatttctt cagcaaacga
     9721 tgttaattga cggtaacatt tttgttcttg tgagtagtag gaagcatgtt catactctga
     9781 ttttgtagtc ttgataagct tttcaatgac tctatcaaat tggtttttta agatttccag
     9841 ttgggattct gaataatggt tttcaggagc tagggactgc aggttattca tcaccctaag
     9901 ccaatatgat aagcgaacgt agttgtaggc cacatcattg taatgaattt tttccaataa
     9961 tgatttgaat acatccaaat tttcatcatc aaaaacaatc ccggcattct taaatgtctc
    10021 aagcatcgaa ttaatctcgg aatagaatgg cttgtcgtaa tcattcaacc agctgtaaat
    10081 ataaggtttc gcttcatctc ctaccaactt ggcaatggag caataatgac cccaatcatg
    10141 gggtttcctc tcatggaggg tctcattcgc tttgtttaat acaggcctaa agtagtgttt
    10201 taatttgaca tctggaatgc ctgcattttg gagtgtctta agtagcatgt agctgttgta
    10261 taaatcaagt ctttcatctg tgtaggtagc tgtcagttta gaaactatct ctaataacat
    10321 tgattcaatg ttatctgtca ggtgattgtc cttatccatt aaagtcatta tattgtgtag
    10381 gttaatcatc tgatataatc ttgattgcaa atcatagatg ttctctggga tgaaagcatt
    10441 tgtattctca atggcttgtt ttgccaattc attgactttg ttgcgcatga attgtatttc
    10501 tccctctaaa cctaattgct gacatagaat aaaggctttt aaaaattcat ttttgcacaa
    10561 aaaatctctt tccttagtgc tgcaatattg aatatcaagc gtaattgctt tttcttttaa
    10621 tgacgttggg gcaatatctg ataatttgta agaaataact ccgtctggag agtatataaa
    10681 gcgcaacaga tcatccttta ttttatcaga gcacaattct ctcaagcgag ggctatattc
    10741 tagtgctaaa aatgcttctt ttgaaaatgg agataattga ttgtcttgaa gccttttgaa
    10801 aacagcctct tcatattctg ctattatttt ttctttagct ttggcaatgg catcagtaat
    10861 tttctccctt aattcaacat tgtagcttaa atcagctggt tgattcactt ttgccaatat
    10921 atttcctttt gttttgtaca tgcttaaaac ttttatgtct tcaatactca tgttataaag
    10981 aagttcatcg taaaaatcat agctattatc tctacccata tgggacagag catttctcat
    11041 gaaatcagaa gacatctcca cccacatatt aacaatattc tcatctgtta atttttctgt
    11101 atcataaggc gttaacttat tccttaagcc ggaatattcg taaattggta aaaactggtt
    11161 agggccaaag cgttcgagga atagtgtttc acccctctct tgtccatagc gttcataata
    11221 agctttacga gtttcttcat attgtctcgc atactgcttc tgtaaataaa tcgcttgcaa
    11281 gagaggggag aggggatggg ctggattgaa atcattatag gcaatttcat ttcctatggt
    11341 tgggtcatta gtaaagaaaa tggcatcgta tttgttaatg tcatataaaa gttcaacgta
    11401 atttgagcca agttgcgttt tcttaccaaa taagcccaat ttcattttat gatgaagatc
    11461 atcaagttcc ctttccagaa tttcacgaga ctctttgtat tctttttctt gtaaatgggc
    11521 cagatggttt ttttgacctc ggccactatc aaaatcctca gctgaaattc tcttaatttg
    11581 gcctatatcc ggattgaaga tcaacatgcc tgcagctgga tagactccac ttccataacc
    11641 gagtatggag gttgagcgca gagggttttt gtatttgaga ttaggggctt cttttctgct
    11701 tttgatgtta atattgtcag agcgcatgga ttttcgcatt tctctttcgg ccaatgtttc
    11761 atctgttaga gtcggtttat tgggtctggg tgtaggcatg gcacttggtg tagtaatgcc
    11821 acgggccaga actctccctt ctctcaatgc cgcagccaga ttttctccag ccattcctgc
    11881 tgtttcatcg aagggagtaa gattaaccaa ggtcatagcc tttaattctt ctatagataa
    11941 cagttttcca tacaatgtgc tgagtattgg ataagcttct gactgcatat ccctgattat
    12001 gcgggtatga gcttcttcat tttcataacc tgctttgatt tctgcttttt tagtaatgta
    12061 tgaatcaccc tggctataga taaggcctct ggcttctacg acaagttcgg ccatttcttc
    12121 taaagcaaca ttgtctttac tggctatatc cttataaaaa tcaagatagg tggcatcata
    12181 ttgatttctt gccctgatga tatcaaggca atcgcaatca tgaattattt tttgatagat
    12241 attttttgca ggaaaatctc cttcttcata cttcgcaaac tgccagaata attgaccata
    12301 ttcgtcctca atgagttgaa aatagccatt ttccggactc ggatccttgt tcgcagttgc
    12361 ctcagctaca aatcttgcag tttctgcatc aatatgtaaa acgcgtgtca gataatagta
    12421 taaaaagata gcactttcat gatcccataa atcttcatca tctccttctc gggccgaatc
    12481 atggaataac aaagcgattt gtatgagttt gatgtctgtt tctgttaaat tttgggcctc
    12541 ctcattacca tgtctcctgt ataaattggc aaaggcacat gcatagtttg cagcacgggt
    12601 aacatgttgt ataccatgat gaaatctagc tatagttgat tcctgatgcg agtcctgata
    12661 ggaataacga taaatattgt cataagccca cctgacatga tcgcccaaat cattttcaag
    12721 atttaaatca ttcgtttcta aagagtcaaa aattgtatat ggtattcctt ttgaatcgag
    12781 tagttgatga atatagggag gttcccaagt ttgttcatct tcgtaaggct cttcatttcc
    12841 agtaagaatt tctaaaggtg atggttctac aagagtgctc tctctcattg agaaatcaat
    12901 tgaaccctca ttaaacatga cgtaattaaa taattcacga gttttaccat tcttggataa
    12961 ttcgttaaat cgctctccta atatccctaa aatatccagg cgagttgttg atgtctctcc
    13021 ttcttgctgg taatctggac tctcttttaa agcatttaat ttttcgataa cagtttctat
    13081 tacatgcaaa tttttttgcg tcactttggg ttgaaaaaaa gggtgtttag cattacgcaa
    13141 tccaaagcct aaatacttgg caagcttgtt atcctgtccc cagctttccc tgctctgttt
    13201 taaactattc aaagttagaa tcaaagaatc tattgtatta tttccctcat atctgtggcg
    13261 ttcataaagc tcaatatatt cgttcatttt catcatacca gaatgccgag atagattaaa
    13321 attatagcaa ttaacttaat tgttgtacaa aaaatccgac cataaagtca tttgaagcta
    13381 tttaaggagt attcctgcta actaaagtgg actacaatta aatttgcccg attgagttga
    13441 gagaacatct tggttaatat gtcatttagt tatttttaat atatgcctga gatactctat
    13501 aaccatgtat actgtaatta tttgcttgtt tatcgctata ttgctacctt atctggcaaa
    13561 agttcccgta gcttatgcca tgcataaagc gggtggctat gataatcgtt atcccagaga
    13621 gcaacaagct cgcttgcaag ggtttggcgc aagagctctg gcagcgcatc aaaatgcttt
    13681 tgagtcttta ctgatatttg caacagcttc attaactgca ttggcgacta attcagtcac
    13741 tttaatgacc caatatttcg ctatcgccta tattatttcc cggctttttt atcatttgtt
    13801 ttatttgctg aattggtctt ccctgcgttc cactgtatgg tttttgggtg taatatgctg
    13861 tctttctatt ttatggctta gtattcctta aattttagaa aggcatatct ttattcaaaa
    13921 aattcaccaa gtgatgattc aaagcgcaaa aactcatcaa tttatcatct aattggttat
    13981 agacacttac tctttatctt tgactttgat atcttaaaaa aagaagttcc agatttttta
    14041 caaggatgtg aatatgtctg acttacaaca attgcctaag actatcctga gattttcttg
    14101 gcattttata aaaaaaagtc cttggcttta tgctttgttt ttcatagcgc cagtggtgat
    14161 ggtgcttgaa acgaatgtga ttccctatgc tttaaaaatg attattgatg ggataacaaa
    14221 tcataaaggg ggaaaagaaa gtatctttca agacattgca ccggctttgt atttgggtgg
    14281 agttgcctgg tttggtgtga ttctggtgtt gcgcttgcat aattggtggc aagcttacct
    14341 tgttcctcaa tttcaagcca atatccgcat gacccttttc ggttatttaa tccagcattc
    14401 ccatcatttt ttttctaatc aattagcggg aaacctggct aataagataa gtgatatacc
    14461 tcgatcactg gaagccattc gtaaaatcgt atcttgtaat gtaaacacca cttttgcggc
    14521 aattatagtt tcaatcgttt taatgctaac cattaatccc tggtttgcgt tcattttgtt
    14581 aagctggatc ataatccaat tatttatcag cttttattca gctaaagaaa tcaataagct
    14641 ggccagggta aatgcggaag ataaaagtat cttaaaggga aagatcgttg attccttgaa
    14701 taatattaat accgtgaaat tatttgtcca aaatgagtat gaaaaaaaat acgtttggaa
    14761 aacacaagat aaagaggtta aaagcaataa gcgccttgtt ctttttatca atttttttcg
    14821 tcttttagtc gatgttcctg tcagtattat gttaattgcc atgatttatt cagtcatcaa
    14881 tttctggcag agaaacttaa tcagcacagg tgacgtggtc tttatatttg ccacttcatt
    14941 tgccattatg aatcaaatgt ggagtctcag taattcactt tgtgatttgt ttgtcgaaat
    15001 agggatagta aagcaggcgc ttgccatatt atcccaacct atagaagttc aagatatagc
    15061 caatgcaaaa gagttgaaag tcactcatgg tgaaatcagg tttgaaaatg tgacctttcg
    15121 ccatagggat ggtcaggaat tatttaccaa taaatcgttg gttatcccgg ctaaacaaaa
    15181 agttgggctt gtaggctatt ccgggggagg caaaacaaca tttatcaatc taattctccg
    15241 ttattttgat gtggatagcg gcagaatatt gattgataat caagacatta gcgagatcac
    15301 acaatattct ttaagaaaat caatcagtat gattcctcag gataccaatc tattccatcg
    15361 taccttatta gaaaacattc aatatggtaa agaggatgct gacagggatg aagtagtaac
    15421 agcagcttct ttagcaaatt gcatggactt tattcagaat ttacctcagg gttttaatac
    15481 tatagtaggg gaaaggggta ctaagttgtc aggggggcaa agacaaagaa tcgctatagc
    15541 aagagcattt ttgcatcata ctcctatatt gattttagat gaagccactt cacaactgga
    15601 ttctttaact gaagaatgca ttcaagaatc cttgcaacca ttatgcgagg gaaaaacaac
    15661 tatcgttgta gcgcatcgac tttcaaccct tcttaacatg gatagaatcc tggtttttga
    15721 taaaggaacc atagtagagg atggcacaca tcaagagttg atagaaaaca atggattata
    15781 taaatccatg tgggacgcgc aaacctcagg tttcttgcct gtggaacaag aagatgaggc
    15841 tgagcttgct aaagaatttg accggcctta tttggcgcaa gtgtaattta accaaaaaaa
    15901 aagcaattgt cgttcaagat gaaccaaata cgcccgttcc aggtagaggc tgatttctta
    15961 ccttaaattt gattgcgagc cgacacccat catctgggtg tcgacattca cttctaaact
    16021 cggtttttca aaataatttt tcaaaaaaat acactggctt tgtctttgct agcattggtc
    16081 ataagcttgg atctgttgct tttggagggc cagctataaa gcgacgatta cgggcaaatt
    16141 tcgggaaaaa aggtccaatc ttaacaataa aatcataagc cataagaaga gcccccaagg
    16201 caaatacgac atcccctgga aaacgaagcc attgccataa taaggtggtg ttatagaatt
    16261 caaggcttcg ggcataggat agtccatgtt catatactgc cattaactgc gaccagccaa
    16321 ttgggaaaaa attcaggaca atccataaaa agagccctgc attatataac caaaaggcat
    16381 aaacacccaa tctatcactc cataccttgc tttcaccagc agcatatctc agacaaaaat
    16441 agattaaccc gagagcaaga agcccaaatg cgccaaataa agcagtatgg gcatgattta
    16501 aagtcaagaa tgttccatgc tcatagtaat taatcagtgg cgcattcagt gtacctcctc
    16561 cgaatacacc ggcaccaaca aaattccaga acgcggctcc caatatgtag agataagcga
    16621 gattgtaggt aaacgttccc tgttttttaa tcaattgtcg atgctcaatc gcatcaataa
    16681 taagaagaac cagaggcaag acttcgataa aggaaaacat ggagcctagc ggaatccaca
    16741 tttcaggagt ccctgtccag taccaatgat ggccagtacc aatgactccg cccaggaaaa
    16801 ttaaaatgga ttcaaaatat accgttctct ctgctgcttt tctggaaacc aaaccagttc
    16861 ccatgagcaa ataagctgta gcacaggcgg cgaaaaactc aaaggattgt tcaacccaca
    16921 gatgcacaac ccaccaacgc cagaaatccg taatggtgaa cgatttttca atccctgtta
    16981 aggggatcat gccaaagcaa tataaaacag cgacattgat agtgctggcc cagaacaggt
    17041 tttcaagctt gatgcgacct gtccagaact caatggtagc tttttttaat ccatcccacg
    17101 taggccacat accgcggaaa acaataaaac tccaaagaaa caatccaatg caaaacccga
    17161 tttgccaaag tcttcctaat tgtatgtaag acaagccttg attacctagc caaaaccagg
    17221 ctttatcaat gtaccccata atccccaggt aattgccaag gatggctcca cccactacga
    17281 gcaaggtgac ataaaacaga aggtcaacta aaaacccttg tcctttggct tccttaccgc
    17341 taatgatggg tgccataaat atgcctgcac ttatccatga aagcccgatc cagataatgg
    17401 gagtttggat atggacgtct cgaaggaaat taaaaggcag ccacgtattg acattaatac
    17461 catagaagct ttcgcgctca gcataataat gagccatgat ggctcctacc gcgatttgca
    17521 gcaaaagaaa caacgcggct acaaggaaat attggcctac tttacgttgg ctttgagtca
    17581 ggggtttgaa acctgaaaaa ataggttcca tgggtccatg atcaacatca cttaaatagc
    17641 gatgataaat gtagataacc gcaccaaatc ccataaacac aaagcaaaat gaaatccagg
    17701 tccaataaaa cgtccctgat gagggggtat taccaacact tggctcgtat ggccaattat
    17761 tggtgtaaga tatgttttga tttgggcggc gagctatcgt tgttaaagag gaataaataa
    17821 gaaagtttgc agtttggagc gcagtatcag gatttaagct ataggcctgg gtataacccg
    17881 ccataaaatt atgattcagt aacgattgac tgatttcagt ttgtaattgc cgtattgagg
    17941 tggcaattgc tggtgtcagg atgctcgttt ttttattgag atcaaggcgt tgcagtgagt
    18001 cctgcatttc tttattaata acgaattgtt cactttctgt taattcggaa taggctttat
    18061 taaaacgaat ttttgccaat tcttcctgaa ccaattctcc caaacgcacc agatattttg
    18121 ctgtgtagtc ctcaccaaaa taggatccca taccgtataa actgccatag tccattaagt
    18181 cagcgcgttg aaaacctgct tttccttcca caatgtcttg tgatgtcatt accgtttcgc
    18241 ctgtagaggt cacaaaacgg tctggtaaag gaggtaccaa tcgataggtt ttaacagtac
    18301 cccagatgag gattagaaaa caaagaatgg cagtgactag taaaacccat tttaaaatat
    18361 tggttattgg gtcaaatgag tgatcctttt gaacaggtgt ttcatttgca atagtatgca
    18421 tcaggctatc cttagcgtaa tggttcaatt cggcattgat tataaagagg atctttacaa
    18481 gaaacaattt acgttagcta caaaactgat tcttaaaaaa ccatacatct ccagtaccca
    18541 taaggttagt ctttgttgaa tagagtctgc ttctcagaaa gaagatgcgg ttcttttata
    18601 aggagaagta gtgcataagg cataattgat gacataaaac aaaacactgc aaccgagcga
    18661 caataacaga gtgtttttaa atagcaataa tattaaaagt atcaatgttg cgcgcgatat
    18721 ttccaagtaa agagtattgc tatggccatc tgcaattcgg ccaagggtat aaagtgtcaa
    18781 taaaattaat gctcccatca gccaggatgt gaccatggag ggatcaaaaa caagagagag
    18841 gtaagtatat aagaacactg caaatgcagt attggttaac atataaatcc caggtgaggt
    18901 cgtatttttt ttaatcgctg ttttttttga gacaggggag ttttcttgtt ccaagcgatg
    18961 aatgatccat tctggcggca taaagaacgc gcgtaacaca tcccatttgc tttttagccc
    19021 tttaccataa tacaggatat ctttaaccac tttgatgttg gcgtaaaaag ggttccatga
    19081 atccaggggt tcggtgaccc cataatcagc cggtgcttct tctggttcaa aagtgccaaa
    19141 caatttatcc cagataatca ggcttcccgc ataattttta tctatgtatt gagggttttt
    19201 gccatgatga actctgtgat gagacggagt attaaatatt ttttcaaacc aacccatttt
    19261 cctaatggat tcagtatgaa tccagaattg atagatggta ttgacagaag cgacaatgac
    19321 aaacatccag gttgggaaac caatcagagc caagggcaaa taaaacaccc aggaagttaa
    19381 tgtctggaaa tagccctgtc ttaaggcaac agataagtta aagtgctcac tttggtgatg
    19441 gaccgaatgg cctatccaga agaaactgtt tctgtggctt actctgtggt accagtaata
    19501 gaaaaaatca accccgatcc ataaaagcaa ccaggaaaaa atcgattgag tattcacatt
    19561 aaaaatagcg taatgctcat ataaataata gtaactataa ataatcaggc ccttgatggg
    19621 gagtgcgcca atttcttcaa ggataccgct gctgaaattg ttgaccgaat cattcagttg
    19681 atagacaccg gattttttat aataggctac ccaagtttca attcctatga ggatcaaaaa
    19741 aagaggtgag gcaaaaacca ttaaatttat atccataaat atcctttgaa cagtgataga
    19801 cgctttgcaa taggtatttt ctattttgca gacgttgagg cctatgcctt attttctaat
    19861 acttttgttt tacttgtaaa taatgatatt gttatcagcc ttgttagaaa tcattctaaa
    19921 ggatatttga tgcatcagga acatctaggc caaaccgata ccccaaaaat agatccttgg
    19981 ttaatatttg tcatattaat gttgatggct ttgatgcaaa ctacaaccga tcaatacatt
    20041 ccttctcttc cagcgattac cacagcattg aacagcactg aagcgtctat tcagttaacc
    20101 atttctatgt ttatgttggg tttaagccta tctcatatct tttatggtcc cttgtcagac
    20161 aaaataggac gcaaacctcc gttaatgttc ggtgttgggc taagtatatt aggtagtttg
    20221 gtttgttttt ttgcgcccaa cgtcagtgtg ttaatcttgg gtcgatttct tcaggggttt
    20281 ggaattgggt gttgcaattc agtggggcga tctttggtga gagatttatt caccgatcgt
    20341 ttgctggcta aaattggttc ttatgtcggt gttgtcagta tttttattat ggcagcttca
    20401 cccactttag gaggatttat tcaagagatg gctgggtgga aagccaattt tatgttctta
    20461 tttgtttttg gaatccttgt ttggctttta gcattttgta ttcttcctga aacgaataaa
    20521 cacttaaatc ctgaagctac aaaaatcaaa gtgatggcga agaattattt taccttatta
    20581 cgtagcaaga tctttgtggg atatacactg tgtgcttgct ttgcttttgc gggaatggtt
    20641 tcttatctta cgattgcgcc atttttattt caaaatacca tgggattgag tcccttggaa
    20701 tttggacgtt taactttttt tattgcgggc gccatctgtt ttagcgggat tgtcaacagt
    20761 cagctggtca tgcgtaaagg aatatccttt atggtttgta ccggtgttct ttttatgatt
    20821 gtcggtggtt taagcctgtt attaattacg atactgggtt ggtcacaaat ctattttatt
    20881 atgatttcaa tgacattatt tagtgctggc gccggattta catttattaa tgcctttgct
    20941 ggtgcgtttc atccttttcc gcaaatggct ggaacggttg gggctttata tgccagcatg
    21001 caggatttaa gtgcagcact gaccagtggt tttattgcgg gaattaaggg gtatggtcaa
    21061 tattctttag ctatgatatt actgatttta ggtttaagtt ctttagtggc atggtattat
    21121 cttggattgc aagatgacgc tatttcaaat taaggggtat taatgaaaat taccgatgaa
    21181 gaattgaaaa aaattatcag caaattatct ggctctaaag ccgatgacat tcaagatgat
    21241 acggccctaa tagaagattt gcatttggat tctttaaaaa ttgttgagct tttggctatc
    21301 ctcagtgaag aatatcaatt agaggtcagt gaagaagatg cgatgaattt tcatacttat
    21361 aaagatttat atgattttac gcaagcctga tagttaccgt ttattccatt ttctgtagta
    21421 aggttggtgt atttcgatca acgactgtca agttaagaag gtggcgaatt ctaatttaac
    21481 aatttgaatt taaaataaaa acagataagg tgctttttga taatttttcg attctcgttt
    21541 tgtcctttgt ccgttccagc tgaggaagtg cttgagcgat gtctcgaagc catgttggtc
    21601 ttgataaagg cttcgagacg gcgtaaacgc ctcctcagcc tgaacggata gtgtaattag
    21661 ttttagcttg acgcctatgg gtaaaatgcc tcaacaaaca atgatcaaat cagataactt
    21721 ccaataaaga acaggctgca attcctcctc cacctgccgc caccaaaagt accttttctc
    21781 cagattttat tttgccatcc gatctcagtc gatccaaagc gatgcctaca ctggatcctg
    21841 aagtatttcc tgtttcttca acagttttta tcgttttttc ttcaggaaaa cctaattgtt
    21901 ttgctaccga caaaaccaga tttttatttc cctgatgagg aacaagccat tggatatcag
    21961 agattgtcag atttaattct tttagaaaat ctttggcgct gtctgccatc ccatgtactg
    22021 cttttacaaa taaagcggta ctttccttta tggtaatgta aaattgctca ttatcattgc
    22081 aaactgcggc aggcaagcgg gaacctcctg ccggagttga gacggcatcg tatacttcac
    22141 cgtcagcaaa caaagcagat gctataaagc gaaagtcagc tttttcttta tcttgagaaa
    22201 cacaacatgc tgctgctcca tcaccgaata aaacactggt tgcaaaattg tttttattta
    22261 agaatttgga gcgaatttcg ctggctatga gcaataccga accgccgttg gtttgcgcta
    22321 aagcagcact tgtatgcaaa ccgacaacaa atccggcaca ggcagcccct atatcaaaag
    22381 ccccggcgtt ttgcaggcca agtctgtatt gtacgagagg gctgcttggc ggggagggat
    22441 aatcccctga gatagtcgcc aggatcactt ggttcacttt atgtttttcc tcgggttttt
    22501 ccataaacaa ttgttctgcc gcctgtacag ccagatcgct acaagcttga tcctcattca
    22561 cccaatgccg gtttttaatt ccagttgaaa acccaatcac ggctggattt tgggtactgt
    22621 ccatccaatt gagaacatct tggttgctca tgattctatc gggaagcgcg acacaagggc
    22681 cttttatgta tatgggtttt tcacaacgga aaaaattcat gacaacaatc cttctgaacg
    22741 cattaaaggc agaccaccac tgactgttac tactgatccc gtcataaacg aggtttttga
    22801 caaaagagga tcaatggctt ccgcaacttg agttggttca cccatggtgc cctgtggaat
    22861 aatttgcatt actttttctt gataggctgg gtttttccaa aattgttctg ttctggaggt
    22921 tttaatcaag cctacgcgta ctatattaga caagatattg ttagcactat aatcaacagc
    22981 caaatttaaa aataaccctt ccagagcagc ctttgccgca caataaacag ggtatcgact
    23041 ggtgccagct gttgctgaaa gacttgaaat gaggactagt cgcccgaatt tattttttag
    23101 caaggcaggt aataaacgat gaataagcca aatgttgcca ttgagattgt tatccaggta
    23161 agtacgaaca gcgtgatagg gcaatgcatg taatttacgc agttgggtga cccgagtgaa
    23221 cgcatttaat atgatgccat caagtggttc tcttaaaatc agttccaaat cgttttcaca
    23281 agcttgagga tctgaaaagt tataaatgaa acctgtcacg tcaatgtttt gttggcggta
    23341 tattgcaagt gtttcatgaa gactgtcgtc gttagaacag gtaatgatga tcttatcgcc
    23401 tgattcactg cgtcgtttcg ctatggcttg ggcaatatca gaggagccgc cagtaatcag
    23461 gatattcatt ttgcccccag ctcaatgagg tattgttcaa tagtattgat cgattcgtaa
    23521 tgccctttct ccagtggaat gacagggatt ttaatttcaa attcacgttc caactcaatg
    23581 attaacaagg ccagcatcaa actatccaag cccccttgct caagttttgc ctgtttgttt
    23641 tcaggaagat tggtaagacc aattcgttcc agtacttgtt caattcgatt acttagcatt
    23701 atttttttcc ttattatcta aaccatcaat attcaaaacc aacgcttttc tatcaagctt
    23761 accatgttga tttctgggta gggatgacat gagctgtaat tgaatgggca atttcctggg
    23821 tagtagatgc gtttgtattt tttctattaa attttgttca gaaatggaag aaggcagcga
    23881 ttttttttcg atacagacga ttaatcgtgt cccatatatc ttgtcttcct gagggataac
    23941 aatggcctct tttatcacat caatttggca aattttttgc tcgatctcag agaggttaac
    24001 tttttctccc ccaatattaa caaggctgtc tcgacggcct ttgattgcga ctagcccatt
    24061 gggtaatatt tctgcaatat ctcctgttga taaccagcca ttgtgtattt tagtactatc
    24121 tttttcacca agataaccca gcatcatttg agtgccgcta attaataatt catgttcagc
    24181 agaaagtttg accttccaat cgccaacagg ataacctgca taattttctg tgaataaagg
    24241 atccttactg gaatacgtca gcactctcgg agacgcttca gttaagccgt aattgttata
    24301 gatgatagcg ttaggaaata tttgtttcaa atccgctctg agtgcggtag ataaaggagc
    24361 gccagcagaa acaatcgcgt taattttggc tgcgctatca gggtatcgtt ttgctatggc
    24421 attgatagct acccagtgag aaggtacgcc actccacatt tgaggaacgg tactttgttc
    24481 cattagcatt ttaatgtctg taaaatgagt gattagtcga gtggtcaggc caaacattaa
    24541 accagggagc aactggccca gaaggccaaa agaataggat aaaggtaaaa agagcaattg
    24601 atcttgaatt tttttaaatt ccagcgcttt tatgaccgca tggcagttgg cttttatgtt
    24661 gatacttgat agttgaactg cttttactga tcctgtgctt cctgaggtga agagaataag
    24721 tgctgtctgg ggatggggct ggatcgtttt attttcacat atttcctgca attccccttc
    24781 ttcatttatc attacggcat tacccaaaag agacagtcgt ctttgctttt cttcctctga
    24841 aatggaattg gggaaaaggg caatggggcg accagtttcc aaacaggcta atagttgaat
    24901 aataaatcca atctcatgtc ttgcatggag gacaatgatg gtttttggaa gtttcaggaa
    24961 ttgctttgaa cttgctaaaa cttcatgtgt taattcatga aaagttattg ctcgattgcc
    25021 ctgagtgatt gctactttat ttggaagatc tggcgccaaa aacagcatga ttttattcct
    25081 caattttatt gagctgccag gtagccacta ttggcagagt tttatccaaa agatgttttc
    25141 ggcaaaaatt cctgcgaatt tttccgctgg tagtatgtgg cattgcttta agaggaatca
    25201 ggactatagt atgtacctcc aattggtggt tttcgtaaac aagctcaaaa atctcattga
    25261 ataaattgtc ttgagctacg tcatccatga atcgattttt tacttcacac atcacagtca
    25321 gtttatattc atgctcctcc tgaatcacaa aagcagcaca tttgcccaat acgtgatgga
    25381 gaggagaatg catcaggctg aactctatgt cctgaggata atgattttta ccataaataa
    25441 taattaagtc tttaatgcgt ccagtaacgt ataactcatt ttcatggaga aagcccaagt
    25501 ccccggttct taaatagatt gcgctacgct catcgtcttt aattttccct gcgaacgcat
    25561 gctttgtttc ttcgggttga ttccaatatc ctttggcgac actgttactt tgtacccaaa
    25621 tttcaccaac ctggtcaaaa tcacatggga tcaaggtatc aggatctata attttaactg
    25681 cttgaatggg attaccactg ctgactaact tgtaactgcc tggactgtta tcgtcagcaa
    25741 aatgtacgcg atgatcttga aattgttctt tggccagagt taatgttttg tatggacttc
    25801 ctggtgttcc tcccgtcact aacaaagtag cctcagccag gccatagcat ggatagaagg
    25861 cttctttgcg aaatccaaac tctttaaatg cctgataaaa atgttccatg gtttctgctc
    25921 gcactggctc agcaccgttg aaagcggtca cccatgaact taaatccagc ccttcttttt
    25981 tttcttccct gattcgcttg acacaataat cgtaagcgaa attggggctg ccactaatag
    26041 tggctctgta tttagtaata tgttttaacc aggaaagcgg gttttgtaaa aatgagaaag
    26101 gggacatcat gattgcctga attccaccat agatgggtgt cagaatgcag ccaatcaaac
    26161 ccatatcatg atgtgggggc agccagctga aaataatggt ttcatcattc atatgaaaag
    26221 aggtaaaaat tttattcaga ttatccagta aattatggtg gcttaccatc actcctttag
    26281 ggtgcatggt tgagccggag gtgtattgta aaaacgcaat gtcattgctc tgaatggagg
    26341 ttggttgcca actactactt ctgtttaact caatgctatc aagcgcaata gcaggaattt
    26401 tcaggaattt gggatttgta tttaattcgt ctgcggtgaa ttttttgatg tgatccgcaa
    26461 tcatcagtac tatgaccggt tttgagttgg taactatgcg ttgtgccttg tccaataatt
    26521 tttcttgggc tggtgggtaa atgggtacag caatgcagcc tgcataaagg cagcccaaaa
    26581 atgcctggat aaggggtaat ccaggtgcaa acaataacaa gaccctatcc ccgggttttg
    26641 ctccttctgc ttgcaaagtt gccgcaattg ctttggcgtg ttgatccaat tgctcataag
    26701 tcatcgtctc ttctaactct ttgttcagaa aagtacagct tttcttgtta gggctgtgta
    26761 aagcacgtaa cctgacgacg tcaaccagag actggcactg caaatattct tttttcacta
    26821 taaaacacct taattgctat atgatgcttt ttgatagttt aggacttgtc ccgcatagga
    26881 tagaccacca ccaacactca tcaggagaat tttccctgat ctgtcaaaca atttttcctc
    26941 aactgccctt gctagtgtgt agagaactga tgcgccacct atattgccta tcgtatcagc
    27001 atcccagatt attgttttgt tgtttaaatg actttcatga agtacttggt ctattaattt
    27061 tcgattgacc tgatgtggga ttacccatgt tatctcgttt ttttcctttt ctgttggcaa
    27121 taaattatca agcatttgca tgtatctgga ataggccaac tctttcatta gggtttcatc
    27181 acccaccaga tgataaccaa ccgaatcgat ttgctcatga gttggcggga ataccccttg
    27241 agtcgtaaat gcgttgacgt attgaccatc agtagagaaa aaactctttt caatggtaaa
    27301 agacccctcc ttgctttttt ctaaaagtaa ggaggcagct ccatcagcca aaccgatcca
    27361 ggttttttca ttctcagggt caatcacttt ggaaagtgtt tcagagcaac aaaccaatat
    27421 tttttgatac cccaaagcca tcattgcgta ggcaaaatgc aaggtcgata aagaggtgga
    27481 gcaaccagtt tttaaatcgt aagcgggcgc atttaatttc aggtttccaa gtacagcagc
    27541 cgcttgagat cccgtaaagc gtttattagt tgtcgagcct aagagaaatg catcgacatc
    27601 ttttgaccta ctttgggtta tcagtttatc tactgcttgc atagctagcg attcagaaga
    27661 cagttctctt gaattatcga gtgattccca gggcttgtgg caccagtatc gagtatgaaa
    27721 gccaaattct tcgcctattt tttctgcgaa tttttcgaga aaaccttgag acttatctgc
    27781 agttctcgga aaattcttta aaatttccag gtttgtgata gggcctgatg caggcagaat
    27841 tcttgatgcg ccagtaattt caaattgtat gttaggtttt aagatattca taaagtggcc
    27901 ttattttgtc tggagcgcat agaggttaca attattgaaa ttacactctt tctatcctac
    27961 cagatcaaga atttttatct ttttcgtata aatagctcta aacttataca attaagagta
    28021 gcctattttg ggaattaagt tattatgagt attaatcaaa tttccaagtt acaaagtctc
    28081 agattccaag atttaaatat tgcaagagga aagaggtatg tcttcccaac atacagataa
    28141 gacgttaatt aaatggatag ataatttaat tgatgaacaa agttttttac caataggttc
    28201 tgaatatatt ccagataata cacctcattc tcttcctggc gctgaggtga ttaccggatt
    28261 agccaaagtg aatcaaaggc cagtagcgat ttatgctcat gattcttcag tcaacagggg
    28321 gtatgttacc cgacaaggcg ctagaaagat tattcattta atggataaag cggaaaaatt
    28381 acgcattccc atcgttgcct ttttagcatc tcccggggtt tctctcgaag aaaatttgct
    28441 gagcggcgac gaatacactc aaattattgc tcgaaatatt agactgtcag gaattatccc
    28501 acaatttgca gtgatactgg cacccactct tggagcgccg gcatattccg ccgtgttaat
    28561 ggacttatta ttttttaaca agcatagaag ctatcttatg gtgactagcc ctacggtagt
    28621 acaacaagcc attggtgaaa aagtgactat gggcgagttg gggagcgcaa cactgcatgc
    28681 aacgacaacg ggtattgctg atttcgtgga cgatagtgtt accgcacaaa taaaccatgt
    28741 gaaagcgata cttggttttt ttcctctaca taccaatgaa cgtccgcctg ttcatcctat
    28801 catggagcct aatcacccca ttcctgatat tcctgcaaat ccgagaatgc catttaatat
    28861 gctcaattta attcaggctg tcgtggataa ttcaaacgtt atccagtaca aaaggactta
    28921 tggacaggcg atgatttgtg cttttgtgca tatacacggc tttgctgttg gggttgtcgc
    28981 taatcaaagc attcgttaca gcggagccat cgattccgat gcagctcaaa aagcagccaa
    29041 atttataaga atatgtgatg cttattctat accgattttg acttttatcg atgttcccgg
    29101 ttttatgccc ggtaaaagag aggagcaaaa gggattattg cagcatggtg caaggttttg
    29161 cgtagctatg caaaccagag tgccaaggct aagtgtcatc gtgcgtaaat gttatggagc
    29221 ggcagctttt ctcatgatgc aaaccaaatc acagaatgga gatttggttt tggctctgga
    29281 aacagcaaat cttggtgtca tggggaagga aacaactaat aaggtgcaag ccatggatgg
    29341 tcaaccagta caggaatcat cacaatccca attgacagaa aagtctcaaa ttgatgattc
    29401 cctcatggaa gcttattcat tgggcttgat tgatgaaatt atccaagcag cgcaaattcg
    29461 cagtcgcctt gcttctcatt tggagttttt atatagaaga atggaaacca tgcccccggc
    29521 aaaacactct attgtataag gaaaataatg acctcttatg aaaatttgat acaactgtct
    29581 gaattttttg agtcatgcct gggtaatcct ctggaagaca attcccctgt taattttcaa
    29641 caaattttgc tcgctgatga gcaggaaata ttagctttgt cagcaataga atttattaaa
    29701 caatgggggt atttggacta taccgtacct cattatttag ggggtaagct gagctccctt
    29761 gatgagctgt atactttaac caggttgttg gccaggcgtg acatcacgat ggctattatg
    29821 tttggattgg ctttctttac tgcattgcca atatggatag ctggaaactc aaagcaaaag
    29881 aaacatttga gtgagagcct tcgccaggga aagataggtg ctttggcttt gactgaagaa
    29941 gagcatggaa ccgatttgac ttccaataag atgaatgcca agccaactga agatggctgg
    30001 gaaatttcag ggcgaaaatg gtgtgttaat tttgcaacct tgagcgagta cgctgtggtg
    30061 ttgtgtcgta ctaatgaaaa gggcgggtta ttaggttttt ctttgtttta tatcgataaa
    30121 aaattagccg aaaagaggat ttctcctacg cccaagttgc caactcatgg agtcagaggg
    30181 ttagatatca gtggtttttc ttttgataaa gtgaaagtct ctaaagacgc attaataggg
    30241 aaggaacaac gtggtttgga aataacctat aaggtattcc agatttcgcg aaccttatgc
    30301 gcctgtttgg ctattggggg cgctgatact gctttgaggc tggcattatc atacagcttg
    30361 caaagacaac tgtatggcaa atcagtgttt gaaatacccg cagtaaaaca gcggcttggc
    30421 gaattgttta ctcttttatt aattgcggat tgcactgctc aagttatggt tcgcgcctgt
    30481 acggtaatac cggaaaaaat gagtctttgg tcagcaatta ttaaattcct tattcctcat
    30541 attggagaag atatagcaga acagtgtgcc attattctag gcgccagagc ttatctgaga
    30601 accacgcaat gggctatttt tcagaaaata agacgcgaca ttcaagtggt tggcttgttt
    30661 gatggaagta ctcaagttaa tttatcaatt attgctggta atttgttgcc tcaggcaggc
    30721 atgcggggta aacatcgggc agagcattcc gaaaaaattg agaaaatttt taatgttcgt
    30781 ttgtcttgtc ctccttttaa ggaggataga ctcggtttgt ttactcatga agaagatgat
    30841 gtattggctg gtatcctgtc attaaactca attccaatca atccattgat cactacaata
    30901 cgtcatgaaa ttgaaaaatt ggatcaagaa gtcatccgtt tacatgatga gaaattattt
    30961 gacccacgca gtttagccgt ttttcgtttg gcagaaaaat attgctggat ttttgctgcc
    31021 agttgttgtt tgcagttctg gcactataat caggagatac tttctgatga tttgcaaaat
    31081 acagattggc tggatttagc gatgcagtta attcttaagc gattgaattc aggttcgatg
    31141 attgatagcg tgttgcaaga agctatgagt gagcgtttgt attcttttta tcaggaaaaa
    31201 cagctgtttt ctattatgcc aatacatatt gcggggtaat gagggatgaa ttggaagaga
    31261 tttttaattg ccgccatgac aaagaaaaaa taccaggttt ttctgaatga taccaagcat
    31321 cctaaagagg cgagagaacg attatggaat tcagaaattg tcccgttgct gaaaaaatca
    31381 tcttattggc agaaactatt aaaagatcaa tcttctgtag ctcttaatga ctttggaatt
    31441 acagaatatg aagattatca ggaaggattg ttggcagccc agcattctac tatccaacct
    31501 tttaatggtg aggaattgat tttttggtca gaaacttcag gaacatcagg agttagaaag
    31561 tttttcccta taacagattc attccagaaa caatttcagc gtacaatggc cccgtatatt
    31621 tacagtctaa ctcagcgatt ccccggcttt tttaaggaaa aaatgttgta tcttgttgcg
    31681 gtagatactc ataaaacgac tcctgccggt attcctacgg gatggattag taattttaat
    31741 tatcgtcatt tgccttcctt tattaaaaaa ttttatgcca tgcccgatga ggtttttgct
    31801 agccatgagg cttacaatca atggggtccc atctacggtt tagcttctga tttaagtgcg
    31861 atatttgcgg tgacaccgat ggtaattgat gctttttatc aacgctgtat tgagggcttt
    31921 aaagaatatc ttccttattt attggggaat aaacctctgc catcgcatct tccacctctc
    31981 aatatgaccc ggaaacgacg aagacatttg caggctttag ccaaggttga ccatctctca
    32041 ttcaaacaac tgtggccatc tctggaaatg gctggatgct ggacctctgg tctttgtgaa
    32101 tatcccgctc agcaattaca aaaattgctg ggttctgaga ttaaattggt agacggtact
    32161 tattcggcaa ccgaggggtg gctaacggtt cctgtagaga ctaacagcgt gggtggaatt
    32221 ttacatccag gtgcacatat tgtggaattt attccagagg gggaggcaat tgaaaaagag
    32281 aatcttttac aatgttggga gttggaagta gggaagaaat acgaagtatt tttgacaaca
    32341 gctatgggat ttattcgcta tagactcaaa gatattgtga aatgtaccgg atatttgaat
    32401 agttcaccaa agcttgagtt ttgttataaa actcaactct tgaaattaga aacctgctca
    32461 attacaggta gagagctgca aagcgcagtg ctggcaactc cgctcaatat tgagccttat
    32521 tggtattttg cgcgcaatag tgtggggaat cgtattgttt tggtaactga tgatgaggta
    32581 gagatttcag agctatcact gaaaaaattg catgagcaat taattcatat tagtccgact
    32641 tatgctcata gcgtggaaac cgaggaagtt ttacctgtga ccttgctgca attgcccaag
    32701 gaaaaattat tagctggcat gcatgggcaa accaaaccga aatttatctc tcaggaggtt
    32761 attgccgaga gataaaaaaa cttaacaata aagtttcaaa atgtggaata agtgtaaaat
    32821 atcggtagat cgaaaaatta tttatcatta aatttattga ttgaaacaa
//
LOCUS       DASLOE010000019        30850 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.019, whole genome shotgun sequence.
ACCESSION   DASLOE010000019 DASLOE010000000
VERSION     DASLOE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 30850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30850
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.019"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..490
                     /locus_tag="U6309_000701"
     CDS             <1..490
                     /locus_tag="U6309_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504155.1"
                     /translation="VATWRFPFVFQGSFKILDRDNYNEHFILYRCEYTKAHQTDILED
                     EFSPLDYQFTYLYAGSVKYHDNILNQLSENAIYLLQYALHLTLSKMLTNKRPSDIYID
                     EIALNFLTERIKQKFDEGKNFESEADIGFAVAMDFNPQEQTALAAVRNLLSCLTVEVN
                     RV"
     gene            complement(519..1388)
                     /locus_tag="U6309_000702"
     CDS             complement(519..1388)
                     /locus_tag="U6309_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEN5504156.1"
                     /translation="MTVKRSSLGRNLSALLSQSSSSLLANEQPQTEQQLKLAVTCLQP
                     GKYQPRGGMEEAPLNELAQSIKKQGVLQPLLVRELDNGKYEIIAGERRWRASQLAGLT
                     EVPVILKQVDDETAMAMALVENLQREDLNAMDQARAMYRLTNEFALTHQQVADLLCKS
                     RTAVSNFLRLLSLSTPVKKLLENGDIDMGHARALLALDEEQQSQVAQLIIAKNLSVRE
                     TEQLVERVKNGKPELKTHHKDNPMFKEQLNSLAKHLQTKIKLKQGKTGKGTLVIHYEN
                     DHNLQKIIEQLLS"
     gene            complement(1402..2172)
                     /locus_tag="U6309_000703"
     CDS             complement(1402..2172)
                     /locus_tag="U6309_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN5504157.1"
                     /translation="MAKVIAIANQKGGVGKTTTAINLSASLAASKQQVLLIDLDPQGN
                     ATMGSGVDKRQLVHTTNDVLLRDCLAEQACLATTCGYDLIPGNEDLTVAEVSLMERNH
                     RETFLFKALQPIQSNYDFILIDCPPALNTLTINAFVAADSVLIPMQCEYYALEGLAAL
                     LSTIEQVKSTVNSRLHIEGVLRTMYDARNRLCAEVSKQLLEHFPAKVYRTVVPRNVRL
                     AEAPSHGMPALQYDKSSPGAAAYMVLAAEVISKQTVAS"
     gene            complement(2175..2801)
                     /gene="rsmG"
                     /locus_tag="U6309_000704"
     CDS             complement(2175..2801)
                     /gene="rsmG"
                     /locus_tag="U6309_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEN5504158.1"
                     /translation="MIDNTKIRSLLEKGLAEFKLDSIGDLLLDFLLLLNKWNKTYNLT
                     AIRDIETMVSKHLFDSLAILPWIKGNHIIDVGTGPGLPGIPLAIAKPDLQFVLLDSNG
                     KKIRFLNEVKRQLNIKNIEPIQIRVENYHPNQGFDTVISRAFSSLEQMIKWTQHLVAQ
                     DGLWLAMKGRFPDTELVPIHQTYRVERYAVPGIEGERCCVLINNTNKE"
     gene            complement(2798..4672)
                     /gene="mnmG"
                     /locus_tag="U6309_000705"
     CDS             complement(2798..4672)
                     /gene="mnmG"
                     /locus_tag="U6309_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEN5504159.1"
                     /translation="MNLEQLYDVIVVGGGHAGTEAALAAARLGVKTLLLTHNIDLLGQ
                     MSCNPAIGGIGKGHLVKEIDALDGAMAKAADQAGIQFRILNASKGPAVRATRAQADRV
                     LYRKAIRTQLQSQANLTIFQQAVDDLKIEGGLVTGVVTQMGLTLKARAVVLTVGTFLG
                     GKIHVGMNQYAGGRAGDPPSIALSKSLRDLDLPVGRLKTGTPPRIDRRTIDFSQMVEQ
                     PGDTPVPVFSYLGAASDHPQQVPCHITHTTEATHDIIRNNLDKSPMYAGVIEGVGPRY
                     CPSIEDKIVRFADKTSHQIFVEPEGLTTEEIYPNGISTSLPFEVQVQFVRTIKGFENA
                     HITRPGYAIEYDYFDPRGLTSFLQTKAIPNLFFAGQINGTTGYEEAAAQGIIAGMNAA
                     LQIKDQELWCPRRDEAYIGVLIDDLITCGTQEPYRMFTSRAEYRLLLREDNADLRLTE
                     KGRQLGLVGDERWDSFSKKREAIESTQALLYNSWVRVHHNDLFKETLFNPMQHDCRAV
                     EFLKRPEINYQHLLMMDDLNLPELPQEITEQIEIQNKYAGYIDRQQQEIEKLRKHENT
                     LLPETLDYNDVVGLSSEVIQKLNRIKPTSLAQAGRISGVTPAALSLLLVHLKKSRLPV
                     "
     gene            complement(4803..6716)
                     /locus_tag="U6309_000706"
     CDS             complement(4803..6716)
                     /locus_tag="U6309_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2888 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504160.1"
                     /translation="MATSSLILQSYLKTLKTDEKSELRYEDKVEELLEYNSNAKGSDF
                     LTPLSFYLPIIENTDEVMLRYMSEENKQKLIRDLQVAYLLLLTQKKYEEEHQKYENIK
                     TYAQHIKRCEELLDHLNYNQVCKEQKRAPSPEHGYASDGYPVKYLSIFLGKELAEMIV
                     DSMDRKTKTIKESMGWFNEKRLYWVWASSLLKVILAALPDDFFNVGQATQVVKAPDPY
                     TGTLSWALYYFRFSLNMFLLLKHTISGPWMSEREKKTPWTERFLTQWDQRKFTLLNDS
                     IWATGNLICFFWLTGKGALGTWGDVLTLALLVFDISIAIWDYEEQKTRHNKEMLAYEE
                     DIKKLKQLISKAEEEPGKEDEKLRIIKEYELQLQGLMRAQKESERNWDLQKVSLYAGI
                     AYAVGLMLAFALLAAPFFPVSGPALLAITITGAVLCLAFTVIYNGVKGGMEVYKAHCS
                     AKEAKEGYNNKIELFKLLLEKNPSLDDNEKKFLFLEIKKLKAETEYQKQMVVFQTMHL
                     LRSVILESFIPAVVFASFVFLPLGIGFAVLGATIGVALATNLMINAAFKPAKEEVKAF
                     DEKEYEAFCKDPDNWSKPSKSKQGFFQSKEKKPLLDNVAAKTQEKEKDRLELLDDESS
                     IPLLLSSKNNPSI"
     gene            complement(6944..8380)
                     /locus_tag="U6309_000707"
     CDS             complement(6944..8380)
                     /locus_tag="U6309_000707"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEN5504161.1"
                     /translation="MTTPLYPIILAGGSGTRLWPLSRQNFPKQFLKLNGELSLIQQTM
                     KRAMGLPGNQTIIVSNDAHYFICQDQLQDFNAQTTYLLEPCARNTAPAIACAAHYLAN
                     TVGKDAVMLVLPSDHWIADDEIWLAAMLEGGQFASENNAIVTFGIKPDSPKTGYGYIE
                     AGTTLSSDIKQVLSFREKPDAHTAAQFVSKGNYFWNSGMFICRAGVYLEELEQFEANI
                     YQFSQQALTLAQHHHDFLRLDLDCFSQCKEESIDYAIMEKTDKAVVIPISIQWSDLGC
                     WTAVADANKQDEQGNTLIGNVIAQDSHNCLINSEELLVTTVGIQDQIIVATSDAVLVA
                     DKRYSQQVKDLVHSLRKDHPHLTQDHQRVPRPWGYYEVLAEGTSFKVKRLMVKPGAKL
                     SLQTHQHRAEHWVIVGGEAEVVNGNNTFRLSKNQSTYIPKNALHRLSNPHNEPLYVIE
                     VQSGSYLGEDDIKRFDDIYLRKEVELAE"
     gene            8470..9156
                     /gene="queC"
                     /locus_tag="U6309_000708"
     CDS             8470..9156
                     /gene="queC"
                     /locus_tag="U6309_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEN5504162.1"
                     /translation="MKKAVVLLSGGLDSTTCLALAKSQGFACYALSFSYGQRHSAELC
                     AATRIAKHMGAADHKIVTLDIALFGGSALTDASIEVPEFKESPEIPVTYVPARNTIFL
                     AMALGYAESIGARDIFIGASSVDYSHYPDCRPEFIESFQSLANLATKAGIEGDRFTIN
                     APLQYLSKVQTIQLGTELGVDYGLTVSCYQANEAGEACGQCDSCTFRKRGFKSAGVDD
                     PTRYQKCVHI"
     gene            9199..9753
                     /locus_tag="U6309_000709"
     CDS             9199..9753
                     /locus_tag="U6309_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2885 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504163.1"
                     /translation="MEFFMWDKIKEWYKNNKSYLTMLLIGSAVVGLSVTFLALFFPPV
                     LAAFASISIFGMAPLAFLTSLHLPLAVLTLGCIVTGAAFGAILGGLVAWKQSISIAKH
                     VYSYFGHKQEHQVEINEEDTYRYLMGHLEGEASESFEVEVDEEFNDYTSTDSAEDRPN
                     PLNFSTDSNHQALDGGYEMTELRI"
     gene            10063..10800
                     /locus_tag="U6309_000710"
     CDS             10063..10800
                     /locus_tag="U6309_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2884 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504164.1"
                     /translation="MTLKQFATGVFYLPAAVFSGLTNLFLGSYAKDKRGNYIQDEDGN
                     YVRNRGLIGLTLDAVKYLGTSVANFISSHQKAISVAFWSSLVVAGAAALTVAFWPAAL
                     AAVVNFSVFGVSIASVVGAGYAAQVAATAGVAAALTSTGVYLGATVVNAFNALRECIS
                     NLRSKKPSSNPEFTPVNDETFEEVRRNQFSGLSKGSEQQQTVQLSSSSSEEEEPVHTT
                     KVFAQPKDVTPEVVENPTSTVSLTGSK"
     gene            complement(10856..11425)
                     /locus_tag="U6309_000711"
     CDS             complement(10856..11425)
                     /locus_tag="U6309_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEN5504165.1"
                     /translation="MANNPRLIIGISGSSGIIYGIRLLQVLQKLPIETHLVISKAGQL
                     TRAYETNISAAELKALADVYHPCTDLTASIASGSFKTLGMIIAPCSMKTLGEIAHGIS
                     SNLLTRAADVVLKERRKLVLLPRETPLHLVHLNNMVCITQMGGIICPPVPAFYNHPQN
                     IDDLINDTVGRVLDLFDLDCGLVKRWGET"
     gene            11704..13716
                     /gene="metG"
                     /locus_tag="U6309_000712"
     CDS             11704..13716
                     /gene="metG"
                     /locus_tag="U6309_000712"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEN5504166.1"
                     /translation="MTSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQ
                     CISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPEN
                     QALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEV
                     CGATYSPTDLINPVSVISGVSPVEKESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKL
                     SEWFEAGLKQWDISRDAPYFGFPIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFD
                     EFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKS
                     RGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIAS
                     RCAGFINKRFDNRLSSELNDQKLYNDLLSARESVIDAFVSRDYARAIRQIMDCADKVN
                     QYIDANKPWVLAKDESKLNEVHAICTMGINLFRILITYLKPVLPMMAKASEEFLNSEP
                     LHWDSIDKPLLNHRINTFKPLMVRVEKEKIEAMLVQSKESLMTTPVKENTPVEDANLI
                     SIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDLGDAQKQVFAGIKKAYDAEELIGRL
                     TVMVANLEPRTMRFGVSEGMVLAAGDGQGIYLLQPDAGALPGMKVK"
     gene            13716..14330
                     /locus_tag="U6309_000713"
     CDS             13716..14330
                     /locus_tag="U6309_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEN5504167.1"
                     /translation="MASVKEIDALLPQTQCGECGYAGCLPYAEALAQGAAPINKCPPG
                     GVETVKALGKLLHVDASPYLKEAIDNTRPPSVAAIKEDECIGCTKCIKACPVDAIIGS
                     SKLMHAIITHECTGCGLCVDPCPVDCIEMVSLPAAGYDKDLARQRYNAKQMRQLRDEH
                     EKQQIYRERKKLSAHAQDHTQDVQAKQNYILEALARVKAKKTYE"
     gene            14323..14958
                     /gene="nth"
                     /locus_tag="U6309_000714"
     CDS             14323..14958
                     /gene="nth"
                     /locus_tag="U6309_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEN5504168.1"
                     /translation="MNKQKSQEIFKRFQEQNPHPTTELQYSTPFELLIAVMLSAQATD
                     VSVNKATAKLFPVANTPQALLDLGLDRLKDYIKSIGLYNSKAQNIMKTCEILIDQHEG
                     QVPGQREALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRVANRIGIAKGNTPLAVE
                     QELLKKVPREFLHDAHHWLILHGRYVCTARKPHCKTCIIEDLCEYKNKNLF"
     gene            15199..16950
                     /locus_tag="U6309_000715"
     CDS             15199..16950
                     /locus_tag="U6309_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2879 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504169.1"
                     /translation="MLQESIMKWFSSVVNGVKTIGSSLWEGSKWVWNNASLSKITTGV
                     FSYTANTTFQVLEQAIALRDAVPTLVNNPQAKKIAYGMGYILVNDVLPLVALNFANNT
                     AQNYFGEGYDEDASWYAPYSLFLSGLALTNYLVKAYTWRQGVQTVAHVAVLDSLGPSS
                     FNSNKKSLPPSICDELDCNYKRKIKGWGREPLVLMGNDALTYGVSLIPYVGEPLSQVL
                     RVYFNGRYIIRLTTPELCERHKYLAMKPESVLGFGLTFEATSMLMDKALESTVGMPPF
                     LYYRALRHLLLLMHINVAAHMELPKSDLKDKASSFDPFNVYERVNRFVADVIFSGLIK
                     RIPIDFKPDKDAEPLIPLSPALKFGTMVLNMDLEKEKKTAPGFFSKAIDKAYVLVLPP
                     VFRGANGLINDPIVFMYWPTIRSGAISAVEFVEGVGKTKTMATLAWAPKTVAAAINLK
                     FGIPKKVTRFVLMLSKEEDFWNLATAFKLWFERHNIKGEPKLITGSNLALNGVTPLQP
                     TPVSVEKEPIVSPHLLITEKDTKEVVPAKSLIPVRSQPQTGVVVTISPDSLFSTRRRR
                     VNPDQEKVAITELSMTK"
     gene            complement(17063..18127)
                     /gene="corA"
                     /locus_tag="U6309_000716"
     CDS             complement(17063..18127)
                     /gene="corA"
                     /locus_tag="U6309_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEN5504170.1"
                     /translation="MNTHKTKASAKAGLLPGTAVYIGEHPPQPTQVTIHIYDKKNYKR
                     LDSFDTRQVKEAYEAGHNIWIDISGLADIEKITKICSEYNIHPLVIEDLLNTRQRPKL
                     DIVDDYIFVVFKLLQSPPDHLSYNTEQFCLIIKQKLLLTFRESEKYNLSSIYQRLNGE
                     HSLTREYGSDYLTYLIMDYIVDDYFNFVETSAQLLETLEDQLINKPETIRLQALYTIK
                     RRTITLRKIIPPLRDIVHLLLTDDGGLINKKYNLYFRDLYDHCIRLVESIDLHREMSA
                     SMLDIYLSTINNRMNESMKILTLFASIFIPLSFIAGVYGMNFKFMPELDWRYGYPMIL
                     VTMVLLAILMLYFFKRKKLF"
     gene            18397..19686
                     /locus_tag="U6309_000717"
     CDS             18397..19686
                     /locus_tag="U6309_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HEN5504171.1"
                     /translation="MNQSLSPQQSPGFLFIAVSMITCLILLINVSFKIILLGGLVFAI
                     NSLICPLIAALFLFALRNCTFKEQRHLLNICLMTLYMFCIGVYVLVNLPAAEYMHDNP
                     AYQVIFEEIPKKFFATTIAFALSFYLPHLLFCTKNDRVLSTPKQCVLLALLGGVSFFC
                     LDFYLLFSAPHAHSFKQIFIDSFMISSLLLLIIGVVYLTFLLNDKQHKGKALIETTEL
                     TLFPIYQYLICFAVTVMLVCLACEYRIIAINKDLVLSASCIFFPITIIISSIVGELWG
                     YQANLKLALALLAAQFIFDMLLMGSVALPSPPFFNLNPFYHYIMPRRLSAASLSLFVT
                     FLGNAMLLHYLKYSKWKINRSLRILIANISVNSLLCLVDYSLLYGGIYPYEQIINLAV
                     SVWQYKLLTALIALPVILWMCKILERNYSLVVQTERN"
     gene            complement(19713..20954)
                     /locus_tag="U6309_000718"
     CDS             complement(19713..20954)
                     /locus_tag="U6309_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HEN5504172.1"
                     /translation="MPSAVIILLIILIAIALRQVVRVIIPIWAIMATGAIAVLLCQQI
                     TPSRAFAAVEPDVMLYLFGVFFISQAAEESGYLAFLTDQIFYYANNGKQTLMLIVFIL
                     GLSSAVLMNDTIAIIGTPIILQLCQSHKNLIKPLLLALAFSITIGSTMSPIGNPQNLL
                     IAIKGEMASPFFEFFKHLAVPTLLNLGIVYFFILFLYRNELKEPIKKPVLPLINDRSN
                     LTLVKMSLLMMLVLTMIKILTDFLHYSEFQLNFSYIALLSSVPIWFSKHRWNYIKRLD
                     WGTLVFFASTFILMQSVWDSGFFQTGMSDSHIDVNHIVVIFIMSIALSQLISNVPLVT
                     IYLPLLMSHNHSNITLLSLAAGSTIAGNLSILGAASNIIIIQNSEKRGIRGFGFLEFI
                     LVGAPLTLLNLLVYISFLQKI"
     gene            complement(20971..22356)
                     /gene="glmU"
                     /locus_tag="U6309_000719"
     CDS             complement(20971..22356)
                     /gene="glmU"
                     /locus_tag="U6309_000719"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEN5504173.1"
                     /translation="MNLQIIILAAGQGKRMYSDTPKVLHHLAGKPLLTHVVETAQQLN
                     PNAIHVIYGHGGEQIKSSLPNLPVHWVHQAEQLGTGHAVLQAMPHIPDDAYVLVLSAD
                     VPLIQVETLQSLIECSQRQNPDHSVLALLVAELENPSGLGRIIRNNQGEIYSIVEEKD
                     ANEQVKNIKEIYSGVCCTLANNLKKWLPQLSNSNAQGEYYLTEIISLAVQNKTPITSL
                     TAKNSFEVQGINNRQQLQQLERIWQQRAANQLMEKGATLADANRFDLRGELYCGKDVY
                     IDINCIFTGKVVLGNGCKIGPNCSLTNVTLGDGCEVYANSVLEGCHIANDCHIGPFAR
                     LRSGTQLASHCKIGNFVETKKAIFDEGTKASHLSYLGDVLLGKNVNVGAGTITCNYDG
                     VNKHQTIIEDGVFIGSDTQLIAPVTVGANATIGAGSTIRRNVPPDELTLTESRQKTIY
                     GWKRPVKRERD"
     gene            22478..23362
                     /locus_tag="U6309_000720"
     CDS             22478..23362
                     /locus_tag="U6309_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2874 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504174.1"
                     /translation="MNIFLKVLKKLMKEKLSELSANLKWKSETIGYNTKLSIDQKNVI
                     DKLEESIVLFKDSGNDEADLLSINALIDTARIQIQSIRESYGEPRDKGESVTCLNNLK
                     NHSNDFLGKLKKFDFDLLNKAYTETPENIVYYHAAIYLGDEIFSPRTGIDYEIRTKKE
                     VQLAVRLQALSERINPSHNLEEQRKRALQVLQDLAQDNQNAIKKDKGFSLPGLSFWGV
                     SVVTPSDWFSSGEGRFGVEFNTAVRTIQGMTESQFERPTVKQTVVKQEETKPSDVKKT
                     EATDEEDEEHSSENTHTL"
     gene            complement(23460..24470)
                     /gene="ansA"
                     /locus_tag="U6309_000721"
     CDS             complement(23460..24470)
                     /gene="ansA"
                     /locus_tag="U6309_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEN5504175.1"
                     /translation="MKKRVLIINTGGTISSIKTSHGYEPSPGFIQKALADIPALKHQD
                     MPDYVIKEYQPLLDSSNMTVTEWNRIARDIAHEYEQFDGFVVLHGTDTMAYTASALSF
                     MLEYLGKPVIVTGSQIPLSEIRNDATDNLITSLWLCAHQPIHEVCIYFNQHLFRGNRT
                     QKISAQRFNAFDSPNYPPLASIGIDIELHQHRLLPHPKAPFHLQTITPHFIANFRLFP
                     GFASDVLEYILNQPLRGLILETYGAGNAQNNDPRFLSLLKEACGRGVIIINCTQCHQG
                     KVEMNQYATGHTLKEAGLISGHDMTPEAAHCKLLYLLSKNLEIPEIKKLIETNLCGEL
                     QI"
     gene            24823..25350
                     /locus_tag="U6309_000722"
     CDS             24823..25350
                     /locus_tag="U6309_000722"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEN5504176.1"
                     /translation="MVIDRAGYRLNVGIILVNDSDRVFWGRRSGHDAWQFPQGGLAPG
                     ETAMQAMYRELHEEVGLDKGDVEILGSTRRWLKYRLPKQYLRHGSEPLVIGQKQKWYL
                     LKLVTSEQKVRLDLSDSPEFDSWRWVDFHEPEQQVIFFKRQVYIQALKELEPLLKKER
                     RTPYGLKRKRGNQRA"
     gene            25351..27645
                     /gene="ptsP"
                     /locus_tag="U6309_000723"
     CDS             25351..27645
                     /gene="ptsP"
                     /locus_tag="U6309_000723"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEN5504177.1"
                     /translation="MLKILKRIVQDVTAASDLKEALGILVQRINTAINAEAVSVYLID
                     NKNAEYVLIATEGLNKQAEFRVRIALDCGLIGLIGRREEPINIENAPSHPDFYHNPLI
                     GEDHLNAFLGVPIIQHRKLYGVLVVQQLEQRYFDDAEESFLITLAAQLGGIIAHAEAT
                     GELAELTQPKKGIGVDKVEATYTALTGIGSVPGIGIGTAVVVYPPADIDAVPRNPVEE
                     LEDEINAFYEALETTRDDIRRLSKRMKETVAEEEHALFDVYLRILDRDSLGAEVEHVI
                     REEKISAQAALATVIKKHVQQFESMGDDYLRERASDFRDLGRRVLAELQRTQREEIVY
                     PKRTILIGEEITAAALAEVPEGQLAGVVSAKGSNNSHVAILARALGVPTVMGLRGLKV
                     ELVSRRALIVDGYYGHVYISPSKSLLAEFKQLAQEEQELNQSLVSLRDKPAETTDNYR
                     VSLQVNTGLAMDAGLSMSVGAEGVGLYRSEVPFMSRDRFPSEDEQTIIYRQTLKAFAP
                     RLVTMRTLDIGGDKILPYFPVEEENPYLGWRGIRITLDHPDVFLIQVRAMMRASEELD
                     NLRIMLPMVTTLSEVEEAVYLIEQAFEELVEEGCEIVKPKIGVMIEVPAAAYLAREMA
                     KRVDFISVGSNDLTQYLLAVDRNNARVAALYDPLHPAMLRTLLKIVEGGHAAGVEVSI
                     CGEMASDPLAVILLIAMGFDTLSMNSASLPRVKWVIRNFAIANARKILAEVLEFEHPA
                     EIRFHLQKALEEEGLGGLIRAGKS"
     gene            27642..28448
                     /locus_tag="U6309_000724"
     CDS             27642..28448
                     /locus_tag="U6309_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEN5504178.1"
                     /translation="MISGELLINGVIYIIIGAFAGIMAGALGVGGGLIVVPGLVYIFQ
                     HSQVIPEDILMHVAAGCSLAVMIFTSYASLKAHNKMDEILLPLFKKLCPGLVLGTILG
                     SIMASFIPTHLLKIIFGLFLLGVAMKMLLDVRVSHTDKFPGYWLNNLVSSSIGSISGL
                     LGVGGGVIIIPYLTYCGVPVRKIAAVSNLGTFAVGLVGTIMFMITGTLDMAKISYSTG
                     YIYWPAVLGVAIPSSLIAPFGAKLNYLLPVNYLRYGFIVILITTGIHMFL"
     gene            28466..29236
                     /locus_tag="U6309_000725"
     CDS             28466..29236
                     /locus_tag="U6309_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN5504179.1"
                     /translation="MLTYPNINPIAFSLGPLKVHWYGLMYLIGFVGAWLLGYWRIKHY
                     KLNWNNDQLSDLIFYSALGVILGGRVGYMLFYDFQEFIHHPWVLFKIWEGGMSFHGGL
                     LGVVIAAWLFCRKYGKTFLEVGDFVAPLVPLGLAAGRLGNFINGELWGRVTDVPWGMI
                     YPHVDDQPRHPSQLYEFGLEGVALFILIWCYASKPRQQGRVSALFLMGYAICRLIAES
                     FRQPDSQLGFVAFGWLTMGQVLSIPMLLIGIWLWWAKR"
     gene            29233..30027
                     /gene="thyA"
                     /locus_tag="U6309_000726"
     CDS             29233..30027
                     /gene="thyA"
                     /locus_tag="U6309_000726"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEN5504180.1"
                     /translation="MKTYLQLLEHILQHGVEKSDRTGTGTLSVFGYQMRFDLAQGFPL
                     VTTKKLHTRSIVHELLWFLRGDTNISYLKENGVTIWDEWADNNGDLGPVYGKQWRSWP
                     TADGRTIDQLSEVVQQIKSNPDSRRLIVSAWNVGELDKMALMPCHALFQFYVANNKLS
                     CQLYQRSADVFLGVPFNIASYSLLTHMVAQQCNLDVAEFIWTGGDCHLYLNHLEQAQI
                     QLTREPLPLPSLAIKRKPASLFDYAYEDFEFLNYQSHPAIKAPIAV"
     gene            30103..30372
                     /locus_tag="U6309_000727"
     CDS             30103..30372
                     /locus_tag="U6309_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504181.1"
                     /translation="MKKIIVFVLLTVVCSAGFTSKLSKFLNKMDAEQKQRDAQEWQQD
                     MNFGDYAFRLKERYVDDRGQRCRDYEFRGRSNPYRHGYFTVCDER"
     gene            complement(30489..>30850)
                     /locus_tag="U6309_000728"
     CDS             complement(30489..>30850)
                     /locus_tag="U6309_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEN5504182.1"
                     /translation="TFDIAWGDMDALGHVNNARYFDYFQEARIDWLRELDIKMTGQTG
                     PVVIHVACTFLKPIVYPATVTIHSKVNSLGNSSMIMDHDLYQEETLMAQGVSKIVWID
                     YTQNKSVPLPDIIRNLV"
BASE COUNT         8823 a         5839 c         6546 g         9642 t
ORIGIN      
        1 ggtagctaca tggaggtttc cttttgtttt tcaaggatca tttaaaatac ttgatagaga
       61 taattacaat gaacatttta tcttatacag gtgtgaatat acgaaagcgc atcaaacaga
      121 tattttggaa gatgaatttt ctcccttaga ttatcaattt acctaccttt atgcgggatc
      181 agttaaatat catgacaata ttcttaatca actatcagaa aatgccatat atttattaca
      241 gtatgcccta catttgactt tgtccaaaat gctaactaat aaaaggccaa gtgatattta
      301 tatcgatgaa attgcactta actttttaac agaacgcatt aaacaaaaat ttgatgaagg
      361 aaaaaatttt gaaagtgagg cagatattgg ttttgcagtg gctatggatt ttaatccgca
      421 agaacaaaca gcacttgcag ccgtgcgcaa tttactgtcc tgtttgacag ttgaggtgaa
      481 tagggtttag atagatactg aatcgcaaat tgcaaagttt agcttaataa ttgctcaata
      541 attttttgca aattatgatc gttttcataa tgaataacta atgttccttt accggttttc
      601 ccttgtttta atttgatttt agtctgtaaa tgttttgcta aagagtttaa ctgttcttta
      661 aacataggat tgtctttgtg atgggttttt aattctggtt ttccattttt tactcgctca
      721 accagttgct ccgtttctct gaccgataaa tttttagcaa tgatcaactg agcgacttga
      781 ctttgctgct cttcatccaa agcaagtaat gctctggcat gacccatgtc tatgtcccca
      841 ttttccaaaa gcttctttac tggcgttgaa agagaaagca aacgaagaaa attgcttact
      901 gcggttctgg atttacataa gagatcggca acttgctgat gggtaagagc aaattcatta
      961 gtgagtctat acatggctct tgcctgatcc atggcattta aatcttcacg ctgcaagttt
     1021 tcaaccagcg ccattgccat ggccgtttca tcatctactt gtttaaggat aacaggaact
     1081 tctgtaagac ctgccagttg gcttgcacgc cagcgtcttt ctcctgcgat gatttcatac
     1141 ttgccattat ctaactcacg aaccagtaag ggctgtaata cgccttgctt tttaatggat
     1201 tgcgcaagtt cattgagagg ggcttcttcc attcctccac ggggctgata cttgcctggc
     1261 tgcaaacaag tcactgcgag tttgagttgc tgttctgtct ggggttgctc atttgctaat
     1321 aaagagctgc ttgattgact taataaagca gataaattac ggcctaaact actgcgtttt
     1381 actgtcatta cttaccccaa attaactggc tacagtttgt ttactaatca cttcggcagc
     1441 gagaaccata taagctgctg ctcccggtga ggatttatca tattgcaagg caggcattcc
     1501 atgacttggc gcttcggcca gtcttacatt gcgtggcaca acggttctat acacttttgc
     1561 agggaaatgc tctaataact gcttggaaac ttcggcgcat aatctgttac gtgcatcata
     1621 catggtacga agcacgcctt cgatgtgcaa acgcgaattt accgtcgatt tcacttgctc
     1681 aatggttgac aacaaagcag ccaatccttc caaagcatag tattcacact gcatgggtat
     1741 caaaacagag tcagcagcaa caaaggcgtt aatggttaat gtattcaaag caggagggca
     1801 gtcaattaaa ataaaatcat aattggactg gatgggttgt aacgctttaa ataagaaagt
     1861 ttcccgatga ttgcgctcca tcaggctgac ttctgcaacc gttaaatctt cgttaccggg
     1921 aattaaatca tatccgcaag ttgtcgcaag gcatgcctgc tcagccaggc aatcacgtaa
     1981 caagacatca ttggttgtgt gaaccaactg tcttttatct accccactac ccatcgtcgc
     2041 attaccctga ggatctaaat cgatcagcaa aacttgctgt ttggatgcgg caagagatgc
     2101 agataaattg atggctgtgg ttgttttacc tactccacct ttttggttgg caatggctat
     2161 gactttcgcc ataattattc cttgttcgtg ttgttaataa gcacacagca acgctctccc
     2221 tcgatacccg ggacagcata cctctcaact cggtatgttt gatgaatcgg aaccaattca
     2281 gtatccggaa accgcccttt catggctaac cacaaaccat cctgagcgac aagatgttga
     2341 gtccatttta tcatttgttc gagactgcta aatgctctgc ttattactgt atcaaaacct
     2401 tggtttgggt ggtagttctc tactcgaatt tgtattggtt caatgttttt tatattgagt
     2461 tgacgtttta cttcattgag aaagcgaatt tttttcccat tactgtccaa taatacaaat
     2521 tgtaagtcag gtttggcaat agccaaaggg atgccaggta aacccgggcc agtacccaca
     2581 tcgataatat ggtttccctt tatccagggt aaaatagcca gactgtcgaa caggtgcttg
     2641 ctgaccatgg tttcaatatc acgtattgca gtgaggttat atgttttatt ccatttattg
     2701 agcaggagca aaaaatcaag caagaggtcg ccgatggaat cgagtttaaa ctcggctaaa
     2761 cccttttcaa gcaatgatct tattttcgta ttatctatca tactggaagc cgactctttt
     2821 tcagatgaac aagtaaaaga gacagcgcgg caggtgttac gccagaaatc cgcccggctt
     2881 gagccaagga agttggttta atccggttta gtttttgaat gacctcactc gataaaccaa
     2941 cgacgtcatt ataatccaaa gtctctggga gcagggtgtt ttcatgcttg cgtaatttct
     3001 caatttcttg ttgttgcctg tctatgtacc cggcatattt attctgaatt tcaatttgct
     3061 ctgttatttc ttgtggcaat tcaggcaaat tcaaatcatc catcattaaa agatgctgat
     3121 aatttatctc cggtcgtttt aaaaattcaa cggcacggca atcgtgttgc attggattaa
     3181 acaaagtctc tttaaacaaa tcattatgat gaaccctcac ccagctgttg tataacaaag
     3241 cttgagtgga ctcaattgct tcacgctttt tggaaaagct gtcccatcgt tcatcaccta
     3301 ccaagccaag ctgtcgacct ttctcagtta gtcttaaatc ggcattatcc tcacgtaata
     3361 gcaaacgata ctctgctctt gaagtaaaca ttctataggg ctcttgcgtt ccgcacgtaa
     3421 tcaaatcatc gataagtaca cctatgtagg cctcatctct gcgcggacac cataactctt
     3481 gatctttgat ttgcaatgcc gcattcatgc ccgctataat tccttgtgct gctgcttcct
     3541 catatccggt tgtcccattg atttgcccgg caaaaaacaa attaggaatg gctttggttt
     3601 gcaaaaatga ggttaatccg cgtggatcaa aataatcata ctcgatggcg taacctggcc
     3661 tggtgatgtg ggcgttttca aaacctttaa tggtgcgcac aaattgcact tgtacctcga
     3721 aggggaggct ggtagaaatt ccattagggt atatttcttc cgttgtcaga ccttccggtt
     3781 ccacaaaaat ctggtgagat gttttatcag caaagcgaac aattttgtct tctatcgagg
     3841 ggcaataacg agggccaact ccttcaatga ctcccgcata cattggcgat ttatccaaat
     3901 tatttcttat tatgtcatga gtggcttctg tagtatgggt aatatggcat ggtacttgtt
     3961 gaggatggtc gcttgccgct cccaaatagg aaaatactgg aaccggggta tcacctggtt
     4021 gttccaccat ctggctaaaa tcaatggtgc gtctgtcaat tcgaggagga gttcctgttt
     4081 tcaaacgccc taccggtaaa tccaaatccc gcaagctttt agataacgca atggatggag
     4141 gatctcctgc cctgcctcct gcgtattggt tcataccgac atgaatcttg ccacccagaa
     4201 aagttcctac tgttaataca acagcgcgtg ccttgagcgt gagccccatt tgggttacaa
     4261 caccagtgac taatccgcct tcaattttca aatcatcaac tgcttgttga aatatggtta
     4321 agttagcctg tgattgcaat tgtgtgcgaa tggcttttct atacaaaaca cgatcagctt
     4381 gtgcgcgtgt ggctcgaacg gcaggtcctt ttgaggcatt aagaattcga aattggatcc
     4441 ccgcctgatc ggcggcttta gccatggccc cgtcaagggc atcaatttct ttaaccaggt
     4501 gacctttacc aatgccccct atggctggat tgcaggacat ttgtccaagc aagtctatgt
     4561 tatgggtcaa taacaaggtt tttacaccca agcgtgctgc tgcaagcgct gcttctgtac
     4621 cagcatgccc tccgccaacg actatgacat catataattg ttcgagattc atgtttaacc
     4681 attagttatg ccaaaattat aaattggatt tttggaccat ctgcaaaaaa gatccaagct
     4741 aacatataaa aaaatgagca attatacact tttttggcaa ttgtcccaag atgtaatgaa
     4801 gattatatgc tgggattgtt tttgctactt aacaacagag gtatactgct ctcatcatcc
     4861 agcagctcta gcctatcctt ttctttttcc tgtgtttttg cagcaacgtt atctaaaaga
     4921 ggttttttct ctttggactg aaagaaccct tgttttgatt tggagggttt actccaatta
     4981 tccggatctt tacaaaaagc ctcatattcc ttttcatcaa aggctttgac ttcctctttc
     5041 gcaggtttga aagcagcatt aatcattaag ttggtcgcca aggcaacacc aattgtggcg
     5101 cccaacacgg caaatccaat tcctaacggt aaaaacacga agctggcaaa aacaacggct
     5161 ggtataaatg attccaaaat caccgaacgt agcaaatgca tggtttgaaa aacaaccatt
     5221 tgcttctggt attcagtttc tgcctttaat tttttgattt ccaggaagag aaattttttt
     5281 tcattatcat ccagtgatgg atttttttcc aataagagtt taaatagctc gatcttgtta
     5341 ttatatcctt ctttcgcttc cttggcggag caatgtgctt tgtagacttc catgccgcct
     5401 ttcacaccat tataaatcac tgtaaaagcc aggcaaagaa cagcgcctgt tatggtgatt
     5461 gccagtaaag ccggtccgga aacggggaag aaaggcgcag ccagtaatgc aaaagcgagc
     5521 atgagtccga cagcataagc tatacctgca tataaactga ctttttgcag atcccaattt
     5581 ctttcagatt ctttttgcgc ccgcattaaa ccttgcaatt gcaattcata ttctttgatt
     5641 attctaagct tttcatcctc ttttccgggt tcttcttctg ccttgctaat taactgcttt
     5701 aattttttaa tgtcttcctc ataagccagc atttctttgt tgtggcgagt cttttgctct
     5761 tcgtaatccc aaatggctat gctgatgtca aagactagca atgccaaagt caatacatca
     5821 ccccaggtcc cgagagcgcc tttgccagta agccagaaaa agcaaatcaa gtttcctgtt
     5881 gcccagatgg agtcattgag caaggtgaat tttctttgat cccactgggt gaggaaccgt
     5941 tctgtccacg gtgtcttttt ttccctctca ctcatccatg gaccactaat tgtatgtttg
     6001 agtagcaaaa acatattgag ggaaaagcga aaataataca aagcccaact cagggttcca
     6061 gtataagggt caggggcttt gactacctgg gttgcctgac cgacattgaa aaaatcatca
     6121 ggtaatgcag cgagtatcac tttgagcagg ctggatgccc aaacccaata caaacgtttt
     6181 tcattaaacc agcccattga ttctttgatg gttttggttt tgcgatccat agaatcgact
     6241 atcatttctg ccagttcttt acccaaaaaa atgctcaagt actttactgg ataaccatcg
     6301 ctggcatagc catgttctgg agagggcgct cttttttgtt ctttgcagac ttgattataa
     6361 ttgaggtggt ctagcagttc ttcacatcgt ttaatgtgct gcgcataggt ttttatgttt
     6421 tcatatttct gatgttcttc ctcgtatttt ttttgtgtga gtaaaagtaa gtaggcaact
     6481 tgcagatctc gtatgagttt ctgcttgttt tcttcactca tgtagcgaag catgacttca
     6541 tcagtatttt caataatggg cagataaaaa ctcagtggcg ttagaaaatc gcttcctttg
     6601 gcattgctat tgtattcaag aagttcttct actttgtctt catagcgtag ttcagatttc
     6661 tcatctgttt tgagggtttt caggtaagat tgaagaatta aactactggt tgccataaag
     6721 tcatctcttt tttaattttt gttaattacc tatataaatg atactataaa aagcaaattt
     6781 gttctttttt ataacatagt atttattttg ggtacaagat gaatgcggtt ggctttacag
     6841 tattgatact aaaatttgca ggaataggga taaagtgtat tgcaacttag gcaagagtgc
     6901 taagcacagt gagtggaatc aagagagctg ttttggatag aattcactct gccagttcaa
     6961 cttcctttct taaataaata tcatcaaagc gtttgatgtc gtcttcgccc agataggagc
     7021 cactttgtac ttcgataaca taaagtggtt cattatgtgg gttacttaat cgatgcaatg
     7081 cgtttttcgg aatgtaggtg gattggtttt ttgataatcg gaacgtattg tttccattaa
     7141 ctacctcggc ctcaccgcca acgataaccc aatgctcggc ccgatgctga tgcgtttgta
     7201 aagagagttt tgcaccaggc ttcaccataa gacgtttgac tttgaatgag gtgccctctg
     7261 ccagcacttc ataatacccc catggtctgg gaacacgttg atgatcttga gttaaatgag
     7321 gatgatcttt gcgtaaggag tgaaccaaat ctttcacttg ttgcgaatag cgtttatcgg
     7381 caactaacac agcgtcactc gttgcaacga tgatttgatc ctgaatgcca acggttgtga
     7441 ctaacaactc ttcactatta atgaggcaat tgtgactatc ctgggcgatg acatttccaa
     7501 tgagagtatt gccttgttcg tcttgtttgt tcgcgtcggc cacagcggtc cagcagccca
     7561 gatcgctcca ttgtattgaa atgggtatta caacagcttt gtctgttttt tccattatgg
     7621 catagtctat agactcttct ttacattgag aaaaacaatc cagatcaagg cgcaaaaaat
     7681 catgatgatg ctgcgcaagg gtcaatgctt gttgactgaa ttggtagatg ttggcttcaa
     7741 attgttccag ctcttcaaga taaacccctg cacgacaaat gaacatgccg ctattccaga
     7801 aataattccc tttactgacg aattgtgcgg cagtgtgcgc gtctggtttt tctctaaagc
     7861 tcaaaacttg ctttatatca ctggataaag tggtgccggc ctcaatgtag ccataaccgg
     7921 ttttggggct atcaggcttt atcccaaatg tcactatcgc attgttttct gaggcaaatt
     7981 gtcccccctc taacatggcg gccagccaga tttcatcatc agctatccag tgatccgagg
     8041 gtaaaaccag catgacagca tcctttccta ctgtgtttgc caaataatgg gctgcgcatg
     8101 ctatcgcggg ggctgtattt cttgcacaag gctcaagcaa gtaagtggtt tgtgcattaa
     8161 aatcttgcaa ttgatcctga cagataaagt aatgggcatc attacttacg ataatggttt
     8221 gattgccagg caatcccatc gctcttttca tggtttgttg aattaaagac aattcgccat
     8281 ttaattttaa aaattgctta ggaaaatttt gtcttgataa aggccatagt cgagtgccag
     8341 agcccccggc taaaattatt ggatatagag gtgtcgtcat cacattccat attgtggcat
     8401 aattgacctg cattttagca ggaattttta agagcaagca attgttccaa ttttttgagg
     8461 ttttgcatta tgaaaaaagc tgtggtcttg ctttctggcg gtttggattc aacgacttgt
     8521 ctcgcgttgg ctaaatctca ggggtttgct tgttatgcat taagcttttc ttacggccag
     8581 aggcattccg cagaactgtg tgccgcaact cgaattgcga aacatatggg tgcagctgat
     8641 cataaaatag tcaccctgga tatcgcttta tttggtggct ccgctttgac tgatgcatct
     8701 attgaggtcc ccgaattcaa ggaaagcccg gaaataccag tcacctatgt cccagcccgt
     8761 aatactattt ttttggctat ggctttgggt tatgctgaat caattggtgc cagagatatt
     8821 tttattggtg ccagttctgt tgattattcc cattatcctg attgtcgccc ggagtttatt
     8881 gaatctttcc aatctttggc aaacttggca acgaaagcag gaattgaagg cgatcgcttt
     8941 actatcaatg ctcctctgca atatctgagc aaagtgcaga ctattcaatt aggtactgaa
     9001 cttggtgttg attacggatt aacggtttcc tgctatcaag cgaatgaagc aggtgaagct
     9061 tgcggacagt gtgatagctg tacttttaga aagcggggtt ttaagagtgc tggagtagat
     9121 gatcccactc gatatcaaaa atgtgttcat atttaaccca cacagttaaa ttaatgatat
     9181 aatccctgaa attttggttt ggagtttttt atgtgggata aaatcaaaga atggtataaa
     9241 aataacaaga gctatttgac aatgcttttg attggatcgg cggttgtagg tttgagtgtt
     9301 acatttctgg ctctgttctt ccccccggtt ctggcggcat ttgccagcat aagtattttt
     9361 ggaatggcac cattggcttt tttaacgtca ttgcatcttc ctctcgctgt gctaaccctg
     9421 ggctgtattg ttactggagc tgcttttgga gcaattttag gaggcctggt ggcatggaaa
     9481 caatcaatta gcattgctaa acatgtttat tcatactttg gtcataagca ggaacatcaa
     9541 gttgagatca atgaagagga tacctaccgt tatttaatgg gtcatttgga gggagaggcc
     9601 agtgagtctt ttgaagtcga ggtggatgaa gaatttaatg attatacttc tactgattct
     9661 gctgaagaca gaccaaaccc attgaatttt tcaacagatt caaaccatca ggctcttgat
     9721 gggggctatg aaatgacaga acttagaatc taaaattcga gcacaaatca aatatatctt
     9781 cagggtttac gaaaaacgtt gatcgcttga atttgatcat ttagcttatt caagcatctt
     9841 aattgatggc attttttgct tctatcttgg gttttcgaaa atcctgatca cctgatcata
     9901 cattgtaaat tttatcgagc tcttggcaaa aacctggttg agatgaagac aataaaatga
     9961 acttacttta cacttttgta acaacatatt gcgcacaatt aattcacatt taggaaatgg
    10021 gttgctacaa tctgctccga tttataacct tggagtaaaa aaatgacatt aaaacaattt
    10081 gcaactggcg tattttattt gccagcagcc gttttttctg gtttaactaa cctattttta
    10141 ggttcttacg caaaagacaa acgcggtaac tacattcaag atgaagatgg taactatgta
    10201 agaaaccgtg gtttgattgg tttgactctg gacgctgtta aatatttggg aacaagcgtt
    10261 gccaatttca tttctagtca tcaaaaggca atttcagttg cgttttggtc ttctttagtt
    10321 gttgctggtg ctgctgcttt aaccgttgct ttctggcctg ctgctttggc tgctgtagtt
    10381 aacttctctg tttttggagt atcaatcgct tcagtagttg gtgctggtta tgcggctcaa
    10441 gttgctgcta ctgccggtgt tgctgctgct ttaaccagta ctggcgttta tcttggcgct
    10501 acagttgtta atgcttttaa cgcattaaga gaatgcatct ctaatttgcg tagcaaaaag
    10561 ccttccagca acccagagtt tacacctgtt aacgatgaga catttgaaga agtacgtaga
    10621 aaccaattca gtggcttaag caaaggttct gagcaacaac aaactgtaca actttccagt
    10681 tcaagctctg aagaagaaga gcctgtccac actactaagg tatttgcaca acctaaggat
    10741 gttactcctg aagtggttga gaacccaact tctactgtat ccctcactgg ctcaaagtaa
    10801 tataaaaata ttacaagcaa gtggtcatta aaaacgcggc tttagccgcg tttttttatg
    10861 tttcgcccca gcgtttcacc aatccacaat ccaaatcaaa taaatcgagt actctcccaa
    10921 cggtatcatt gattaagtcg tctatgtttt gcggatgatt ataaaaggcg ggcactggag
    10981 ggcagataat gcctcccatt tgtgtaatac acaccatatt gttcagatga acaagatgca
    11041 aaggtgtttc cctcggcaag agcaccaatt ttctgcgttc tttaagaacg acatcagcgg
    11101 ctcgggtcaa caggttgcta ctgataccat gcgcaatttc acctaaggtt ttcattgaac
    11161 agggcgcaat gatcatgcct aaagttttaa atgagccgct ggcaatggat gcggtgaggt
    11221 cagtgcaggg atgatagaca tctgctaaag ctttgagttc tgcggcacta atatttgttt
    11281 cataagcacg ggtcaattgc cctgctttgc taatgactaa atgcgtttct atgggtagtt
    11341 tttgcaagac ttgtaatagt ctaatcccat agattatgcc tgatgagcca ctgatgccta
    11401 tgataagtct tggattgttg gccatgtgta ttcactcgtt gaaaatttaa tttgatcagg
    11461 acttacgacc ccccaatctc tttattaaaa acatttttta ccttgacctt gtggtttctc
    11521 gtagtcctgt tgatattaac aaattataag agaataagta gtacttaaat tatcggatca
    11581 tctcggtaaa agttaacact tcataaataa ctaccaataa aaacctgttt tctattgcag
    11641 catttccatt gtctttgcgt tataattagt cgctttatta taggtgatga gtattctata
    11701 actatgacta gtgaacgaaa aatgttagtg accagtgcac tgccttatgc taatgggcat
    11761 ttgcatctgg gacatttggt tgagcatatt caaacagata tttgggtccg tactcacaaa
    11821 atgttgggta ttcaatgtat tagcgtttgt ggggatgacg ctcatggaac acccattatg
    11881 ctgaaagcag agcaactggg aattactccg gaagctttaa ccgccgaaat taagttaagt
    11941 catgaaaagg attttaaagc cttcgccatt gattatgatt attaccatac cacccattca
    12001 cctgaaaatc aggcattggc gactaccata tttgaacgtt tgcaggcagg cggtgatata
    12061 gtcaagaaaa caattcgcca gttttatgat cccgtaaagc aaatgttttt gccggacagg
    12121 tatgtgaagg gaacttgccc caaatgtgct gctgtcgatc agtatggcga taattgcgag
    12181 gtttgtggcg ccacttattc gccgacggat ttgattaacc ctgtttctgt tatatcggga
    12241 gtcagcccag tagaaaaaga atcagagcat tatttttttg atttgccgcg ttacgaagag
    12301 ttacttaagg attggacaag aaaggggcat ttacaaaccg aagtaacgaa taaactcagc
    12361 gaatggtttg aggcgggttt aaagcaatgg gatatttctc gcgatgctcc ctattttggc
    12421 ttccctatcc ccggtgttcc ggataaatac ttttacgttt ggttagatgc tcccataggc
    12481 tatatggcaa gctttaaaaa gtattgcgat gagcgtggcg tttcttttga tgagttttgg
    12541 gataaggctt caaaaacaga attatatcat tttgtaggca aggatattgt gtattttcat
    12601 gctttgttct ggccagctat gttagctgcc agcggtttca gaacacctac ggccgtgtat
    12661 acccatgggt ttttaacggt tgaggggcag aaaatgtcca aatcgcgcgg tacttttata
    12721 gaagcacgcg cttatctggc tcacttgcat cctgaatatc tgcgttacta tttcgctgcc
    12781 aaattgaatg gtcgagtaga cgatttggat ttgaattttg acgattttgt caatagggtg
    12841 aatgcggatc tggtcgggaa ggtagtgaat attgcaagcc gatgtgcggg atttattaat
    12901 aagcgttttg ataatcggtt gagctcagag ttgaatgatc aaaaattata caacgacttg
    12961 ctgagcgcaa gagagtccgt gattgacgca tttgtttcaa gagactatgc gcgggcaata
    13021 agacaaatca tggattgtgc tgacaaagtc aatcaataca tcgatgccaa taaaccttgg
    13081 gtattggcta aagatgaaag caaattaaat gaagttcatg ccatatgcac aatgggaatc
    13141 aatttattcc gcattttaat aacctattta aaaccagtct tgccaatgat ggccaaagct
    13201 tcagaagaat ttttaaattc tgagccttta cactgggatt ctatcgataa acctttgcta
    13261 aaccatcgta tcaatacttt taagcctttg atggttagag tagaaaaaga aaaaattgaa
    13321 gcgatgttgg ttcaaagcaa ggaatctctg atgactaccc cagtaaaaga aaacacgcca
    13381 gttgaggacg ctaacttaat cagtattgag gactttgcca aagtcgattt acggattgca
    13441 aaaattgtca atgcagagcc tgtggaaggc gcagataaac taatgcgctt gattttagac
    13501 ttgggtgatg ctcaaaagca ggtttttgcc ggcataaaaa aggcttatga tgctgaagaa
    13561 ttgattgggc gtttgacagt aatggttgct aatttggagc cccgcactat gcgtttcggt
    13621 gtttctgaag gcatggtatt ggcggcaggg gatgggcagg gaatttattt attacagcct
    13681 gatgcaggag cacttcctgg catgaaggtg aagtaatggc ctctgttaaa gaaattgatg
    13741 cgttattgcc gcaaacccag tgtggggaat gcggatatgc aggttgtttg ccttatgctg
    13801 aagcgcttgc ccaaggagcg gcccctatta ataaatgccc tcctggtggt gttgaaactg
    13861 taaaagcttt gggaaaatta ctgcatgttg atgcatctcc atatttgaaa gaagcaatag
    13921 ataatactcg gcctccttca gttgctgcta tcaaagagga tgaatgcatt ggttgtacta
    13981 aatgcatcaa ggcctgtcct gttgatgcaa ttattggtag tagtaaatta atgcatgcca
    14041 ttattactca tgagtgtact ggatgcggct tgtgtgtgga tccttgtcct gttgactgca
    14101 ttgagatggt tagtttgcct gcggctggat atgataagga tttggcccgc cagcgttata
    14161 atgccaaaca aatgcgccag ttgcgggatg agcatgaaaa acagcaaata tatagggaaa
    14221 gaaagaaact ttcagcccat gcacaagatc atactcaaga cgtacaggca aaacaaaatt
    14281 atattcttga ggctttggct cgggtgaaag caaaaaaaac atatgaataa acaaaaatca
    14341 caagaaattt tcaaacgttt tcaagaacaa aaccctcatc ctacaacgga gttgcagtat
    14401 agtaccccct ttgagttatt aattgccgtc atgctttcag cccaggcaac ggacgtcagt
    14461 gtgaacaaag cgacagctaa actattcccg gtagcgaata ccccacaagc cttactggat
    14521 ttgggattag atagactaaa agattacatt aagtccatag gtctttataa tagcaaagca
    14581 caaaatatca tgaaaacctg tgaaatactt attgatcagc atgagggaca agttcctggc
    14641 cagagagagg cgctggaagc attgcctggt gttggccgaa aaactgcaaa tgtggttttg
    14701 aatacagcat ttggtcagcc aaccatagcg gtcgacaccc atattttcag agtggctaac
    14761 cggattggca tagcgaaggg taatacaccg ctggcggttg agcaggagtt attaaaaaaa
    14821 gtgcctcgtg agtttttaca tgacgctcat cactggttaa tattacatgg ccgttatgtc
    14881 tgtacagcac gtaaaccaca ttgcaagact tgcatcattg aagatctttg cgaatacaaa
    14941 aacaagaatt tattttaaga caaagccata atgaaggaaa atcaaacgat aatgaacaat
    15001 ctatgcctca taaaaatgac tggctgttgt atttcaaatt cattctatca aatcatccgg
    15061 gttgagatta gcttttgtac agttaaaaca caaaagttca attgaccatc agctcttata
    15121 aaaaatttca ataaagttca agaaaagtgc tctttccctc tggtatttcc caatttttac
    15181 tcataaaatg aaattccgct gttgcaggag agcattatga agtggttttc atcggtggta
    15241 aatggagtca aaacaattgg gtcaagcctg tgggaaggtt caaaatgggt ttggaacaac
    15301 gcctcattgt caaaaatcac gactggtgta tttagctata ccgcaaatac aacttttcaa
    15361 gtgttagagc aggcaatagc cttacgtgat gctgttccca ccttggtcaa caatcctcag
    15421 gcgaagaaaa ttgcctatgg aatgggttac attcttgtta acgatgtttt accgttagtt
    15481 gctttaaatt ttgcaaacaa tacggcacaa aattatttcg gagagggtta tgatgaggat
    15541 gcatcatggt atgcgcctta ttctcttttt ttatcgggat tagctctaac gaattatctg
    15601 gtcaaagcat acacctggcg gcaaggagtt caaactgtag cacatgtagc ggttcttgac
    15661 tcattaggcc cttcctcatt taattcaaat aaaaaatctc ttcctccttc aatctgcgat
    15721 gaattggatt gtaactataa gcgtaaaatt aaaggttggg gaagagagcc tcttgtttta
    15781 atgggtaatg atgcactgac ttacggggtt agtttaattc cttatgtagg tgagcctctc
    15841 tcgcaagtat taagagtata tttcaatggc cgctatatta ttcgtttaac tacaccagag
    15901 ctgtgtgagc gccataaata tttggccatg aaaccagagt ctgttttagg atttgggtta
    15961 acctttgaag cgacaagtat gttaatggac aaggctctgg aatccactgt gggcatgcct
    16021 ccatttttat attacagggc tctgcgccat ttattgctgc tgatgcatat taatgttgct
    16081 gcacatatgg agttgcctaa atctgatttg aaagacaaag cttcgtcttt tgatccgttt
    16141 aatgtttatg aacgcgtcaa tcgctttgta gcggatgtca tattttcagg tttaattaaa
    16201 aggataccca ttgatttcaa accggataaa gatgctgagc cgttaattcc attatctcca
    16261 gctctaaaat ttggcactat ggtgttgaat atggatttgg agaaggaaaa gaaaacagct
    16321 cccggatttt ttagcaaagc catagataaa gcgtatgttt tggttttacc gcctgttttt
    16381 cgcggtgcta atggcttaat caacgatcca attgtgttta tgtactggcc tactattcgc
    16441 tcaggagcta tttctgctgt tgaatttgta gaaggagtgg ggaaaaccaa aacgatggct
    16501 actttggcat gggcaccaaa aactgtagca gccgccatca atctcaaatt tggaatccct
    16561 aaaaaagtaa cacgctttgt tttaatgtta agcaaagaag aagacttctg gaacctggct
    16621 acagcattca aattgtggtt tgagagacac aatatcaaag gtgaaccaaa attaattaca
    16681 ggttccaatc tggctttgaa tggggtgaca cctcttcagc ctactcccgt atctgttgaa
    16741 aaggaaccca tagtttctcc ccatctacta attacggaaa aagatactaa ggaagtagtg
    16801 ccagcaaaaa gcctgattcc tgttcgatcc caacctcaga ctggagttgt agtgacgatt
    16861 agccctgata gtttattttc aaccaggaga cgtcgggtta atccagatca agaaaaagtt
    16921 gcgataacag aactgagcat gactaaataa attttagagg cttttctgac aagagggcaa
    16981 cagaagtgtg ttgcccggta aagaccctac ccagttctat cgatgatatt atgcaggttg
    17041 taaatggggc gctttccgcc acttaaaata atttttttcg tttaaaaaaa tacagcatga
    17101 gtattgccag aagtaccatg gtaaccagaa tcatagggta accgtatcgc caatccagct
    17161 caggcataaa tttaaaattc attccataaa ccccggcaat aaaacttaaa ggaataaaaa
    17221 tactggcaaa caaggtcaga attttcatgg attcattcat gcgattattt atagtagaca
    17281 ggtatatgtc tagcatactt gccgacattt ctctatgcaa atcgatcgat tctaccaggc
    17341 gaatgcaatg atcatataag tcccggaagt aaagattata ctttttattg attaaccccc
    17401 catcgtctgt aagcaacaaa tggacaatat ctctcaaagg aggaatgatt tttctgaggg
    17461 taatggttct tcttttgatg gtgtataacg cttgtaacct aatcgtttct ggtttattaa
    17521 ttaattgatc ttccaaggtt tcaagcagtt gggctgaagt ttcgacaaag ttgaaataat
    17581 catcaacaat atagtccatg atcagatagg tcaaataatc acttccatat tctctggtta
    17641 acgagtgctc tccattaagg cgttgataaa tggaagataa attatatttt tcagattctc
    17701 tgaatgtgag taaaagtttt tgcttgatga ttaaacagaa ctgttctgta ttgtaagata
    17761 agtgatcggg aggggattgt aataatttaa aaacaacaaa aatataatcg tctacaatat
    17821 ccagtttggg tctttgtctg gtattaagca aatcctcgat aaccaagggg tgtatgttgt
    17881 attctgagca aattttagta attttctcga tgtctgcaag acctgaaata tctatccaaa
    17941 tattatgtcc agcttcatag gcctctttca cttgtcgggt atcgaaggaa tctaaacgct
    18001 tataattttt tttgtcataa atatgtatcg ttacttgagt tggttgcggt gggtgttcgc
    18061 ctatatatac ggcagtcccc ggtaagagtc ccgcttttgc tgaagccttt gttttatgag
    18121 tattcatgaa gttatctcag tttatttatt ttcttattaa cttatcattt agtcatcgtt
    18181 taagtcgaca gttgacgact ctcgtttatc ttgctatgca aagggctata atgctatatt
    18241 tgcgacctga ttagcttaat aaagggcaat tattatttga agcttatagg gatgttgtta
    18301 atacttccat gtaaatcata aagaattaac cccctatagt cagtctctat caataaggca
    18361 gaatgagttg ttatatacta tggaagctct tgtcgtatga atcaatcgtt atcaccccaa
    18421 caatcccccg ggtttctatt catcgcagtg agtatgataa cttgcctgat attattaatt
    18481 aacgtttctt ttaaaattat tctactcggg gggcttgtat ttgcaataaa cagcttgatt
    18541 tgtcccctta ttgcagcgtt atttttattc gcattgagaa attgtacctt caaagagcaa
    18601 cgccatttac ttaatatttg tttgatgact ttatatatgt tttgtattgg ggtttatgtg
    18661 ctcgtcaatt tacctgctgc tgaatacatg catgataatc cagcctatca agtaattttt
    18721 gaagaaatac ccaagaaatt ttttgctaca actattgctt ttgcactcag tttctatttg
    18781 cctcatttat tgttttgcac taaaaatgac agggttttat caactccaaa gcaatgcgtg
    18841 ttattagctt tgctgggggg agtaagtttt ttttgcctgg atttttatct attattttct
    18901 gctccacatg ctcacagttt caagcaaatt ttcatcgatt cattcatgat ctcgtctctt
    18961 ttgctcctaa ttattggcgt agtttattta acatttttat tgaacgacaa acaacataag
    19021 ggcaaagcat taattgaaac cacagaacta acgctttttc caatttatca atatttgatt
    19081 tgcttcgctg tgaccgtgat gttggtgtgt ttggcctgtg agtatcgaat catagcaatc
    19141 aataaagact tggttttatc ggccagctgt attttctttc caattactat cattatcagt
    19201 tctatagttg gcgaattgtg gggttaccag gccaacttga aattagcatt ggctcttctt
    19261 gcggcacaat ttatttttga tatgttattg atggggagtg ttgcactacc ttctcctcct
    19321 ttttttaatt tgaatccttt ttatcattac atcatgccac gcaggttatc agctgcatcg
    19381 ttgtcattgt ttgtcacttt tcttggtaat gcaatgctat tgcactattt aaagtattca
    19441 aaatggaaaa ttaaccgatc cttgcgaata ttaatcgcta atatatcagt caactcgtta
    19501 ttatgcctgg ttgactacag tttgctttat ggtggtattt atccttacga acaaattatt
    19561 aatttagcgg tgagtgtttg gcaatacaaa ctattgacag cgttaattgc tctcccggtc
    19621 attttatgga tgtgcaagat tttagaaaga aattattcat tagttgtgca aacagagagg
    19681 aattaaataa acaaatttaa tggttcattc tcttatattt tttgaaggaa tgagatatac
    19741 actaatagat taagtaaggt aagtggggct cccacaagta taaactccag aaacccaaaa
    19801 cccctgatac cacgtttttc actattttga ataataataa tgttacttgc tgcccccagg
    19861 atagagagat taccggcaat agtacttccc gcagccaggc tcagcagggt tatattggaa
    19921 tgattatgac tcatcaataa aggcaaatat attgttacta atgggacatt agagatcaat
    19981 tggctcaggg cgatactcat tataaaaatc actacgatgt ggttgacatc aatatgagaa
    20041 tcactcattc cggtttggaa aaagccgcta tcccatacgc tttgcattaa aataaaggtg
    20101 ctcgcaaaaa aaactaaagt tccccaatcc agtcttttta tataattcca tcgatgcttg
    20161 ctaaaccaaa ttggtacaga cgacaagagt gcaatataac taaaattcag ctgaaattct
    20221 gaataatgca ggaaatccgt caagattttg atcatagtca gaactaacat catgagcaag
    20281 ctcattttaa ctaaagtaag attgctacgg tcatttatca aaggtaggac tggtttttta
    20341 ataggttctt ttaattcatt tctgtataaa aaaagaataa aaaaataaac tatacccaga
    20401 tttaacaaag taggtacagc caagtgtttg aaaaactcaa aaaatggcga agccatctct
    20461 cccttaatag caatcaataa attttgtgga ttgcctatag gactcatggt gcttccaatg
    20521 gtaattgaaa atgccaacgc caacagcaag ggtttaatta aatttttatg tgattgacac
    20581 agttgtaaaa tgataggtgt acctataatg gcaatggtat cattcattaa caccgcggag
    20641 cttaaaccca gaataaaaac aatcaacatt aacgtttgtt ttccattatt tgcataataa
    20701 aaaatttgat cggtcagaaa agcgaggtag ccactttcct ctgcggcttg tgaaatgaaa
    20761 aaaacaccga acaaataaag catgacatcc ggctcaacgg cagcgaaagc gcgtgatggt
    20821 gttatctgtt ggcagagcaa aacagcaatg gctcctgttg ccataattgc ccaaattgga
    20881 atgataactc tgacaacctg ccgtaaagca attgcaataa gaataatcag aagaatgatg
    20941 acagcagaag gcatttctgt cctcttttat ttaatccctc tctcttttta ccggcctttt
    21001 ccagccgtag atggtttttt gtcgtgattc ggttaatgtt aactcatcgg gcggcacatt
    21061 ccggcgtatg gtgctccctg ctcctattgt tgcgttagcg cccacggtaa ctggagcaat
    21121 taattgagta tctgacccaa taaaaacacc atcttcgata attgtttgat gcttattaac
    21181 cccatcgtaa ttacaagtaa tggttccagc gccaacgttt acattttttc ctaacaggac
    21241 gtcccctaag taactgagat gactggcttt cgtgccttca tcaaaaatcg cttttttagt
    21301 ttcaacaaaa ttgccgattt tacaatggga agctaattgc gtccctgagc gcaagcgggc
    21361 aaaaggaccg atgtggcagt cgtttgcaat gtggcagcct tccagtacgc tattagcgta
    21421 aacttcgcac ccatcgccta atgtcacatt agtcagactg cagtttgggc caattttaca
    21481 gccattacct aaaactacct tgcctgtaaa aatgcagtta atgtcgatat agacgtcttt
    21541 gccacaatat aattctcctc tcaaatcaaa gcggttggca tcagccaggg tcgcgccttt
    21601 ttccatgagt tgattggctg ctctttgctg ccagatgcgt tcgagttgct gaagttgttg
    21661 gcgattatta atgccctgta cctcaaagct gttcttggcg gttaatgacg taatgggtgt
    21721 tttattttgt acagccaatg agataatttc agttaaataa tattcgcctt gtgcgttact
    21781 attacttagt tgaggcaacc atttcttcag attatttgcc aaagtacaac acaccccgga
    21841 ataaatttct tttatattct ttacttgctc atttgcatcc ttttcttcta caatgctata
    21901 aatttcacct tggttgtttc tgataatacg cccaagaccg cttggatttt ccagttctgc
    21961 aaccaggagt gctaaaacag aatgatctgg attttgacgt tggctgcatt caattaatga
    22021 ttgaagagtt tccacttgta tcaggggaac atcggcagat aaaaccagaa cgtatgcatc
    22081 gtcagggata tggggcatgg cttgcaaaac agcatggcca gtccctagtt gctctgcctg
    22141 atgcacccaa tgaacaggta agttggggag tgaactttta atttgttcac caccatgacc
    22201 ataaatgaca tgaattgcat tgggatttag ttgctgagcg gtttccacga cgtgggttaa
    22261 cagcggtttg ccagctaggt ggtgaagaac tttgggcgtg tccgaataca ttcttttacc
    22321 ttgccctgca gctaaaataa taatttgtaa attcatgaga tttgtgtttt cctaagttaa
    22381 atatcataat cggtgctgat ttaaacagtc tgccccaatg ttatgaatga tgtaaagagc
    22441 tatatgataa ataagttgca gttaaaggag tagagcaatg aatatatttc taaaggtttt
    22501 aaagaaatta atgaaggaaa aattgagtga gttatccgcc aatttgaaat ggaagtcaga
    22561 aaccattggg tataatacca aattatcaat cgatcaaaag aatgtcattg ataaactcga
    22621 agaaagtatc gttcttttta aagattcagg taatgacgaa gcagatttgc tgtcaatcaa
    22681 tgcattgata gatactgcca gaatacaaat tcaatccata agagaatcct atggtgagcc
    22741 gagagataaa ggggaatctg taacctgtct caataatttg aaaaatcatt ccaacgattt
    22801 tcttggcaaa ctaaagaaat ttgattttga cttgttaaat aaagcttata cagaaacacc
    22861 tgaaaatatt gtctattacc atgctgctat ctatctggga gatgaaattt ttagccctag
    22921 aacagggata gattacgaaa tccgcaccaa aaaagaagtg caattggcag taaggctgca
    22981 ggcactaagc gagcgaatta atccaagcca caatttggaa gaacaaagaa aaagagcgtt
    23041 gcaagtgttg caagatctcg ctcaggacaa tcaaaacgct atcaagaaag ataaaggttt
    23101 ttctcttcct ggtttaagct tttggggtgt ttcagtagtt acaccatcgg attggtttag
    23161 ctcaggagaa gggcggtttg gcgttgaatt taatacggcg gttcgcacca ttcagggaat
    23221 gactgaaagc cagtttgaaa gaccgacagt aaaacaaaca gtggtaaagc aagaagagac
    23281 caaaccatcc gatgtaaaaa aaacagaggc aacagacgag gaggatgaag aacattcatc
    23341 tgaaaatact catacattgt aatttacttt caatgggtgt attttgattt ctctgcctcg
    23401 aatgcatacg agaggtctcc aaaagatact ttctgttgga gcaaggagat ccctcgttct
    23461 taaatctgca gttcaccaca caaattggtt tcaatcagtt ttttgatttc agggatttcc
    23521 agattcttgc ttaataaata cagcaatttg caatgagccg cctcaggtgt catatcatgg
    23581 ccgctaatca agcctgcttc ttttaaagta tgtcctgttg cgtattgatt catttccact
    23641 ttcccctggt ggcattgggt gcaattaata ataataaccc ccctgccgca agcctcctta
    23701 agcaaactta aaaatcttgg atcattgttc tgggcattac ctgccccata ggtttcaagg
    23761 attaaacctc gtaaaggttg atttaaaatg tattcaagaa cgtcagaggc aaagcctggg
    23821 aacaggcgga agtttgcgat aaagtgagga gtaatggttt gtagatggaa cggtgctttg
    23881 gggtgtggca ataaacggtg ttgatgtaac tcgatatcga taccaatcga tgccaagggt
    23941 gggtagttgg gtgaatcaaa agcattgaag cgttgggcac tgattttttg ggtgcgattg
    24001 cctcgaaaca aatgttgatt aaagtagatg catacttcat ggataggttg atgagcacaa
    24061 agccataagg aagtaattag attatctgtc gcatcgttac gaatctcgga taaaggaatc
    24121 tgtgaaccag tgactatgac gggttttccc aaatactcca acataaatga caaagcggaa
    24181 gcagtgtaag ccatggtatc agtgccatgt aatacaacaa atccgtcaaa ctgttcgtat
    24241 tcgtgagcaa tatctctggc aatcctgttc cattccgtga cggtcatatt agaggaatca
    24301 agtagcggct gatattcttt gataacgtag tcaggcatgt cttgatgttt tagagcagga
    24361 atgtctgcca acgctttttg aatgaatccc ggtgaaggct cataaccatg cgatgttttt
    24421 atgctactga ttgtgccgcc agtattgata attaaaactc gttttttcat gcggatttat
    24481 aatcaatgat ttattattct ttcgataagt atagcgaaaa cttggctata ccaataacaa
    24541 ctaatttcaa gacttgcgaa aagccccaat ctctttggta aaaaagtgtt taattcggtc
    24601 atttagttta tagaaactcc ctcgttaaaa acattaaagt tatctgtctc caaggtattg
    24661 agagtcatta tgatgagtaa tttttgttct ataataaatc aaaaaatgca atatgaaaac
    24721 agggaataaa ttagtccaaa taatatttac agatttaatt gtaagctctt gtactttgtg
    24781 gaaaaatcaa gttattaacg gtttattcta agcagtgaca tcatggttat cgatcgtgcc
    24841 ggatatcgct tgaatgtagg tattatactc gtcaacgatt ccgatagagt tttttggggg
    24901 agacgaagcg ggcatgacgc ttggcaattc ccgcaaggtg gattggcacc aggcgagaca
    24961 gccatgcaag caatgtatcg tgaattgcat gaagaagtgg ggttagataa aggtgatgtt
    25021 gaaatattgg gttcgaccag gcgttggcta aagtatcgac ttccgaagca atatttgcgt
    25081 catggcagtg agcctttggt gattggccag aaacaaaaat ggtatttgtt aaagcttgta
    25141 acaagcgagc aaaaagtcag attggatttg agtgattctc ccgaattcga tagctggcgg
    25201 tgggtggatt ttcatgagcc tgagcaacaa gttattttct ttaagaggca ggtttatatt
    25261 caagcgttaa aagaattaga acctttgtta aaaaaagaac gccgtacccc ttatggctta
    25321 aaacgtaaaa gaggtaatca aagagcttag atgcttaaaa tacttaaacg gatagtacaa
    25381 gatgttaccg ctgccagtga tttgaaagaa gcacttggca ttttggtgca acgtattaat
    25441 acagccatta atgcagaggc tgtttccgtt tatcttattg ataataaaaa tgctgaatac
    25501 gtattgatag ccacggaagg tttgaacaag caggcagagt ttagagtcag aattgctctt
    25561 gattgcggtc ttatcgggtt gataggacga agagaggaac ctatcaatat agaaaatgct
    25621 ccctctcatc cggattttta tcataatcca ttaatcggcg aagatcacct gaacgctttt
    25681 ttaggcgtcc ctattattca acatagaaaa ttatacggtg ttttagttgt acaacagctt
    25741 gaacaacgtt attttgatga tgctgaagaa tcatttctta ttaccttggc ggctcaatta
    25801 ggcgggatta ttgctcatgc agaggcaaca ggtgaattgg ccgagttaac acaacctaaa
    25861 aagggtatag gcgtcgacaa ggtagaagcg acttatactg cactgactgg aataggcagt
    25921 gttcctggta taggaattgg tactgccgtg gttgtgtacc cacctgcgga tattgatgct
    25981 gtccctcgta atccggttga agaattggaa gatgaaatca atgcgtttta tgaagctttg
    26041 gaaacgacaa gagatgatat caggcgttta agtaaacgca tgaaagaaac tgttgctgag
    26101 gaagagcatg ctttattcga tgtgtattta agaattttgg atagggatag cttgggtgct
    26161 gaagtagagc atgtgattcg tgaagagaaa atcagcgctc aagctgccct ggctacagtg
    26221 atcaagaaac acgtgcagca atttgaaagc atgggcgatg attatctgcg cgaacgagcc
    26281 agcgatttcc gtgatttggg ccgaagagtt ttggcggaat tacaacgtac ccaaagagaa
    26341 gaaattgttt atccgaagag aaccatttta ataggagaag aaattactgc tgccgctctg
    26401 gcagaagttc cagaagggca attggctggt gtcgtttcag ctaaaggctc caataactcc
    26461 catgttgcta ttttagcgag agctctcggt gtacctactg tgatggggtt gagaggatta
    26521 aaagtagagt tggtatcgcg tcgggctttg attgttgatg gctattatgg gcatgtttat
    26581 atctctcctt ccaaatcatt attagcggaa tttaagcaat tagctcaaga agaacaggaa
    26641 ttaaaccaaa gcctggtaag cttgcgtgat aaaccagctg aaactactga taactacaga
    26701 gtctctttgc aagtcaatac cggcttggca atggatgccg gtttatcaat gagtgttggc
    26761 gctgaaggag ttggtttata ccgttcagaa gtaccattta tgagtcgaga tcgattccct
    26821 tcagaagatg agcagaccat tatttacaga cagactctca aggcctttgc tccacgtttg
    26881 gtgactatgc gtaccctgga tattggtgga gacaaaattc taccttattt tccagttgaa
    26941 gaggaaaacc cttatttagg atggcgtgga ataaggataa ctcttgatca tccagatgtc
    27001 ttcctgattc aggttcgtgc catgatgcgt gccagtgagg aactggataa tttacgtatc
    27061 atgttgccta tggtcacaac acttagcgaa gtagaggaag ccgtttattt gattgaacag
    27121 gcatttgaag agctggtgga agaggggtgc gaaatagtaa aacccaaaat tggtgtgatg
    27181 attgaagtgc ctgccgctgc ttatctggca agagaaatgg cgaaacgagt agattttatt
    27241 tcggtgggta gtaacgatct tacccaatat ttattggctg tggatagaaa taacgcccga
    27301 gttgccgcac tttatgatcc cttgcatcca gccatgttgc gtacattgct aaaaatagtc
    27361 gaaggagggc atgcggctgg tgttgaggtg agtatttgtg gcgaaatggc cagcgatcct
    27421 ttagcggtga ttttgttaat tgctatgggt tttgacacct taagcatgaa ttccgccagt
    27481 ttacccaggg tgaaatgggt tattcgcaat tttgctatcg cgaatgcccg taaaattctg
    27541 gcagaagttt tggaatttga acatcctgct gaaatacgat ttcatttgca aaaggctcta
    27601 gaagaggaag gattaggggg attaatcaga gcaggaaagt catgatatcc ggtgagttac
    27661 tgattaatgg agtgatttac atcattattg gtgcttttgc aggaataatg gcgggcgctc
    27721 tgggcgttgg tggcgggctt attgtggtac caggactcgt ctatattttt caacactcgc
    27781 aggtgattcc agaagatatc cttatgcatg tcgctgcagg ttgttcactg gcggtgatga
    27841 tttttacttc ttatgcatcc ttaaaagcac ataataaaat ggatgagata ctcttacccc
    27901 tttttaagaa gttatgtcct ggtttggttc ttggtactat tttaggttcc ataatggctt
    27961 cttttattcc aacccattta ttgaaaataa tatttggact gttcttgctt ggcgtagcta
    28021 tgaaaatgct tcttgatgtc cgagtgagcc acactgataa atttccggga tattggctta
    28081 ataatttagt cagtagttca ataggttcga tatctggttt attaggggtt ggaggaggag
    28141 tcatcattat cccttatctg acctattgtg gagttccagt cagaaagatt gccgcagtat
    28201 ccaatttggg cacttttgca gttggcctag tgggtacaat tatgtttatg attactggca
    28261 cattagacat ggcaaaaata tcgtattcca cagggtatat atattggcca gcagttttag
    28321 gggtagcaat tccaagtagt ttgattgcac catttggggc gaaattaaat tatcttttac
    28381 ccgtaaatta tttaagatat ggatttatcg tgattttaat taccaccgga attcatatgt
    28441 ttttgtgaaa ggaatttgaa taaccatgct cacctatcca aatattaatc caattgcttt
    28501 ttctttaggg ccattaaaag ttcattggta tggtttgatg tatttaatcg gttttgttgg
    28561 cgcctggcta ctgggttatt ggcgcatcaa acattataaa ttgaactgga ataacgacca
    28621 gttaagtgat ttgatctttt atagtgccct tggtgttatt cttgggggac gagtaggtta
    28681 catgttgttt tatgactttc aggagtttat ccaccacccc tgggtattat ttaaaatatg
    28741 ggaaggaggg atgtctttcc atggtggttt gcttggagtg gtaattgccg cctggctctt
    28801 ttgccgtaaa tacggaaaaa ctttcttgga ggttggagac tttgtcgccc ctctagttcc
    28861 tttaggttta gcggcaggtc gcctgggaaa tttcattaat ggggaattat gggggcgtgt
    28921 cactgatgtt ccgtggggta tgatatatcc tcatgttgat gatcaaccaa gacacccctc
    28981 tcaattgtat gaatttggtt tggaaggggt cgctttattt attttgattt ggtgttacgc
    29041 atcaaagccg agacaacaag ggagagtgag cgctttattt ttaatgggat acgcgatttg
    29101 caggttaatt gctgaaagct ttaggcaacc tgattcgcaa ttgggttttg tggcatttgg
    29161 ctggctgaca atggggcagg ttttatccat ccctatgtta ttaattggaa tttggttatg
    29221 gtgggctaag cgatgaaaac atatttgcaa ttgttggagc atattttaca acatggggta
    29281 gaaaaatccg atagaacggg cactggaaca ttatctgtat ttgggtatca aatgcgtttt
    29341 gatttggccc agggattccc gcttgttacc actaaaaaac tgcatacgag aagtattgtt
    29401 catgaattac tgtggttctt gcgtggtgat accaatattt cttatcttaa ggaaaacggg
    29461 gtaactattt gggatgaatg ggcagataat aatggtgatt tagggcctgt ctatgggaag
    29521 caatggagaa gttggccaac tgctgatgga cgcaccattg atcaattaag tgaagtagtg
    29581 caacaaataa agagcaaccc tgactctcgt cgcttgattg taagcgcctg gaatgtgggt
    29641 gaattggata aaatggcttt aatgccttgt catgccttgt ttcagtttta tgttgcaaac
    29701 aacaagctat cctgtcaatt atatcaacgc tcagccgatg tatttttagg agttcctttc
    29761 aatatcgctt cttattcgtt gcttactcat atggttgctc agcaatgtaa tttggatgtg
    29821 gctgaattta tatggaccgg gggagattgt catttgtatc ttaatcattt ggagcaagcg
    29881 caaatccaat taacaagaga acctttgcca ttgccctcct tagctattaa gagaaaaccg
    29941 gcttctttat ttgactatgc ttatgaggat tttgaatttt tgaattatca atcacatccc
    30001 gcaattaaag cgcctatcgc cgtttaatta ttatcagcat tggattttta ttgccaacta
    30061 tggtttaata ttgcctcaat tgtttatttg aggctcattg ctgtgaaaaa aattatagtg
    30121 tttgtattgc tgactgttgt ttgtagcgca ggctttacga gtaaattgag taaatttctg
    30181 aataaaatgg atgctgagca aaaacaaaga gacgctcagg aatggcaaca ggatatgaac
    30241 tttggagatt atgcttttcg cttgaaagag cgctatgtcg atgatcgcgg gcaacgttgc
    30301 cgtgattacg aattcagagg acgctccaac ccctatcgac atggttattt tactgtgtgt
    30361 gatgagcgtt gatttggctg aaaatactct ttctgtatat taaattatcg tctgctcctt
    30421 acggtcgcag ctcggattat aactaaaact gagccgtgac cgtaagagag cgattgtccg
    30481 aataggctct acactaaatt cctaataata tcaggcaaag gcacggattt attttgggta
    30541 taatcaatcc aaactatttt actcacacct tgtgccataa gcgtttcttc ctgatacaaa
    30601 tcatggtcca taatcatgct ggaattgcca agactattta ccttgctgtg tatggttaca
    30661 gtagcaggat aaacgattgg tttcagaaag gtacaggcca catgaatgac aacaggacct
    30721 gtttgacctg tcattttgat gtcaagctcc cggagccaat caatccgggc ttcctgaaaa
    30781 tagtcaaaat agcgagcatt gttaacatgt cctaatgcgt ccatatcgcc ccaggcgata
    30841 tcaaaagttt
//
LOCUS       DASLOE010000020        30260 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.020, whole genome shotgun sequence.
ACCESSION   DASLOE010000020 DASLOE010000000
VERSION     DASLOE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 30260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30260
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.020"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            193..1899
                     /gene="ankY"
                     /locus_tag="U6309_000729"
     CDS             193..1899
                     /gene="ankY"
                     /locus_tag="U6309_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkY/LegA9"
                     /protein_id="HEN5504183.1"
                     /translation="MHSLYLDVVKVMAKIILVYANCVNSTAKGDFALAGQIAKDLVRE
                     KTSDDIDVVLTSTLDGIAKFESLYGKAVDGRVMIEGTSVGLCALELFDSVDNEVVAFI
                     EANRCKYAPSDILKRVLSPDSKFLFIGAANQKAIAKGDKFTKSWLYISHKGDQPNVYE
                     HFDEDDSLIQSVGLGDDRLGLPSLPKVDELPEMNSTQSQKIPSGEYGFMYLAAVHGID
                     DVYLMNQYMSLTEMGQYVLVGEYAKQSKEVQMVLHSLQYKGSSVSLAMPQIFFHDSVD
                     NCLMRNMVSKSSGPLVVSTGVMSSIEAMQDGKLTYYQTMSNNTQFVASYLIAVKSICS
                     SDTTLFGSMPKLIIDLSNLLFAEKPLKESQVKEIRTLLGMSSVTSRLGEMNKRIIAKA
                     NGTVAGELLSFIGKPKTTQSHKQCISVCMSLRKSGETTSPIFDQALRRAAAWGRLFEL
                     KVLIASMSTSDLNKKDKAGNQYTALHWAVMQGHLDCARLLVKSGASVDIQDKSGKSPL
                     HYAIKRGDKETIKLLVQAGASLEIMDDSGAKPCDGSESWVPEYIKSCYEKSGHSKFIS
                     LF"
     gene            2080..3624
                     /gene="ankG"
                     /locus_tag="U6309_000730"
     CDS             2080..3624
                     /gene="ankG"
                     /locus_tag="U6309_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkG/AnkZ/LegA7"
                     /protein_id="HEN5504184.1"
                     /translation="MNSLWIASGSKDMIYVLICIMLVCGLVVYQLMPKLLSYSTSLIS
                     KKQDTSELTHDGITNVLTQLGHPKFEGVCYGFTLNWALAVAQGKESFFYRQLHYLRTH
                     QFDLPETLQQVKEKKERNQSLSKDEVVIETLPQLGKKICVAQDPLQYKEKYRKLVWQP
                     DINSILKAINADSSVAKHIFYKTHSFLNQDEATEYLELLKRTGIREDVAVIISTADHA
                     MGFKLAGNVWRFININDLYQQDKNKPYFEFSSRNLVKELYRVCAENLQGSRLTVNTDF
                     VSVNPEEKLSRALQNVFPVFPVRTKTSYPERLAFFSMAATQGDMNSVKKCIHSGWSIF
                     SRQRLSDDSPILTAIYLGRRDVVRAMLSTSRHRVNQKRKSDSSTLLHIACRYGGSGIV
                     EDLLNIRGIKIDPQDSKGRTPLMYACKKSVVTEDKKLFNLLFAKGASLSIKDNDGLTA
                     LDHALKNEHTLAIQMIEERLEKEACARENSTSRRFKFSETKGTLFQRGVKTISYQSQQ
                     PGFGMK"
     gene            3961..5364
                     /locus_tag="U6309_000731"
     CDS             3961..5364
                     /locus_tag="U6309_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN5504185.1"
                     /translation="MSNKKHSLTIFSLTMITVGSVDSIRNLPATALFGSQLIFYFILG
                     ALFFLIPTALVSAELASGWARQGGVYIWVKEAFGKKSGFLAIWLQWIENVIWYPTILS
                     FVAGTIGYLINPSLTTNPYFLWAVIVSCFWGATIVNLRGMKSSAMFSNICSLAGLLLP
                     MSLIIGLGAVWIAQGNPLQINFDMPSIVPHLSDKGMWVSLTAIILSFCGIEIATVHAN
                     DVKDPQHAFPKALVYSVGIILSTLILGSLAIAVVLPQKDINLVAGIMQAFSAFFLKYH
                     MDWMMPVVAVMLVLGGLGGVSNWIIAPTKGLLVAAEEGNLPEVFQRTNGKGAPAMMLY
                     TQATIVTVLSALFLFMPSVNGSYWLLTALAAQLYMLMYLIMFVAAIKLRISQPYHPRP
                     FKIPGGMAGMLFVAGIGIIGVVTTLAVSFIPPEGINVGSATRYELTLIIGLLLMCAPP
                     FITSWLQSKEADLEPAV"
     gene            5354..5944
                     /locus_tag="U6309_000732"
     CDS             5354..5944
                     /locus_tag="U6309_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0405 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504186.1"
                     /translation="MLYNLTRQVNFNNPSCLCNFQNMGYIDELLEELSARLPELEWKI
                     SGLSSSISIHNLPKGIFSSVIEFNGPACIKEIQDDIHALQKHKDESIACFLAERIQKK
                     INVLVILCQMDKKNNKPESKISFDLTTLSTRQQWIKTMEEKICALEEQYQAMRKTLEQ
                     MKSCSNPATILHLQTELGNVEKRLTLAKETLNRAIS"
     gene            6148..6489
                     /locus_tag="U6309_000733"
     CDS             6148..6489
                     /locus_tag="U6309_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN5504187.1"
                     /translation="MSDKFSHITKDITTQLAKFRKEMPELMTGFSSLAQAATKDGALD
                     KKTKELIAMALAVAKQCPGCIGFHSQTLVKLQATREELLETLGMAVYMGGGPSLMYAA
                     EALEAFEEFSK"
     gene            complement(6704..7147)
                     /locus_tag="U6309_000734"
     CDS             complement(6704..7147)
                     /locus_tag="U6309_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEN5504188.1"
                     /translation="MRLLFISLILASGYLQADTINNYMNIANNIPQMEMKADPQAQAW
                     ARSARHVLTITSESIAETLIQANEAAKTQGKPIFCLPQGVQLNSFVMNELIQQTYKEI
                     SSQQSDKDKMTVSQVALLGLSKKYPCEPSPQEKQIQHVASLLSPQ"
     gene            complement(7166..7714)
                     /locus_tag="U6309_000735"
     CDS             complement(7166..7714)
                     /locus_tag="U6309_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEN5504189.1"
                     /translation="MNKQMQWHLFGNTSGLLIICCLLILAFLDQFIFYELPCPICILQ
                     RICFVAVGLCFVMNLMLGIRASHYGLMLLASLLGLSISVRQMYIHLTPGDPGYGGLFL
                     GLHLYTWSAIIFLVIILLIAGALILDKGLMPDYKVRSKSAKVMIWVFFILILANGIST
                     FLECGPHECPDNPSKYYLLNNN"
     gene            complement(8131..8334)
                     /locus_tag="U6309_000736"
     CDS             complement(8131..8334)
                     /locus_tag="U6309_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEN5504190.1"
                     /translation="MFTKIFIIFVMILIVASLASGLFYLVKDTDKSKRTVKALTVRIA
                     ISLSLFVFLFIAFKLGLIQPHGI"
     gene            8402..9130
                     /locus_tag="U6309_000737"
     CDS             8402..9130
                     /locus_tag="U6309_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEN5504191.1"
                     /translation="MPSLTCFNFRFTSNWPMLILTAVCFFLFISLGFWQIHRADEKTE
                     MITAQQALAKQEPITWQPGQKLPEQYQRISIEGAFLPKLFLLDNQHYQHQFGYDVVSP
                     MLLDDDSIVMVDRGWVSGDITRRTFPNVQTPNGKFKLFGMVYFPSKKQWVLGPSFEEK
                     ENKVTLLELIDEEILKQLLQKKVYPFIIRLDKNEPFGFVREWEIVSMPPQRHFAYAIQ
                     WFAMALVILIIFVIFNLKKNEKTI"
     gene            9114..9659
                     /locus_tag="U6309_000738"
     CDS             9114..9659
                     /locus_tag="U6309_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504192.1"
                     /translation="MKKPYKYMTLLLLVVIFAAPGITAYLFYKNPSWLGTSKVNKGIL
                     LSPPIALKPLDSSAKWRIIYWNPNGCDKTCLKQLNMLGRVRLSFGRKLYQVDEWLLLS
                     NKDSLIAEELKPILKEQDIHVAALSLTEMSKLSTLYPEAKIFIANPDNYLILSYQPGC
                     NPGDIYKDLKLLLNTTEIKSG"
     gene            9664..10671
                     /locus_tag="U6309_000739"
     CDS             9664..10671
                     /locus_tag="U6309_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEN5504193.1"
                     /translation="MSNSSIRLAATLAVFLAFFVVMLGAYTRLTDAGLGCPDWPGCYG
                     RMVLPGADKGLQEAQSQYPDLPIESKKAWTEMAHRYAAGTLALLIFFIAFQVWFKRRQ
                     DQDLPWHLPLALFMLVVFQAALGMWTVTLKLLPVVVMGHLLGGMLIVACLCRFRLQIA
                     QLKGQNLPEWRPWLRLGIVIILLQIALGGWVSANYAGISCIGFPLCNGTWFPELHFAK
                     GFNLFSTIGANYQGGVLESEVRASIQFIHRIGAILTATYLLTLSLIIVFKTHSIYLRA
                     TAIIMAMLVLLQFTLGIMNVIHLLPLKVAVAHNGVAALLLAVAFCSLHFTQDKEVSHA
                     Y"
     gene            10661..11545
                     /locus_tag="U6309_000740"
     CDS             10661..11545
                     /locus_tag="U6309_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HEN5504194.1"
                     /translation="MRTEYAARLPVDWRDYVELCKPRVVLLMLLTVIVGMYLAAPGWV
                     SLRLIAFTLLGIGLCAGSAAAINHLVDRHIDSIMARTKKRPVAYGRVSVKQALWFAVI
                     IGLMGLSLLILFVNQLTALLTFVTLIGYAGVYTGYLKRATSQNIVIGGLAGAAPPLLG
                     WTAVTGQLDPQALLLVLIIFTWTPPHFWALAIYRYKEYQDAEIPMLPVTHGIQFTKLN
                     IYLYTVLLLVVSFLPFVVSMSGWIYLLGALALGIRFLVWAHKLYFTDKPVVAMQTFRF
                     SILYLMLLFVFLLVDHYF"
     gene            11555..12196
                     /locus_tag="U6309_000741"
     CDS             11555..12196
                     /locus_tag="U6309_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEN5504195.1"
                     /translation="MNIKIKGGAVTITVLALVSLLAGIYISQNFHFKKKINVADFHGT
                     YLENPRTVNNFSLTGTDQKAFNNSSLQGRWTLVFFGFTNCGYLCPTTMAELGKMYRIL
                     EKKGVKNLPQVVMISIDPQRDSLDKLGSYVTSFHKSFYGARGEEDSIKSMTHEMGIAY
                     AKINNKESSEPENYDIQHSGAVMLFNPQGELNAFFTTPHHADLLAKDYVLLVS"
     gene            12562..13470
                     /gene="rimK"
                     /locus_tag="U6309_000742"
     CDS             12562..13470
                     /gene="rimK"
                     /locus_tag="U6309_000742"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEN5504196.1"
                     /translation="MKIAILATNPHLYSHKRLKAEAEAAGHEVKIINPLYCYMNVAAS
                     NPKVHYRGGAPLPHFDAVIPRIGASITYYGTAVLRHMETMGMYTLNESIAISRSRDKF
                     RSLQLLARKGIPMPLTSFAQSPDDTEDLIHMVGGAPLVIKLLEGTQGKGVILADSHQS
                     AVSIINAFKEMHANILVQEFIEESRGTDIRCFVIGEKVVAAVKRQAKDGEFRANVHQG
                     GKAVKVKLSPQERAIAVSAAKTMGLRVAGVDLIRSNHGPLVLEINSSPGLEGIEKATN
                     INLAGKIIEYIEKKAKPISSNHRFHG"
     gene            complement(13474..13719)
                     /locus_tag="U6309_000743"
     CDS             complement(13474..13719)
                     /locus_tag="U6309_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504197.1"
                     /translation="MLNKRFSERLNKELDNIGVPEATAERIEILSKLIKIPKFKAEAL
                     LNGITSPDEAVLKTLAQELEVNADWLIGKSDSKSGAH"
     gene            14038..15528
                     /gene="zwf"
                     /locus_tag="U6309_000744"
     CDS             14038..15528
                     /gene="zwf"
                     /locus_tag="U6309_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEN5504198.1"
                     /translation="MLLKAESKYPCDLILFGTLGDLSCRKLLPALYQLELANLLHEKT
                     RIIGCAREELDFSAYVDLIKNKIQQFLFDEIDEAVWLRFIKKLAYCKLDLNQSDDYQK
                     LTDYVNPSERVVISYLAIPPSLYSQTCRSLARLNLTQKPSRVVLEKPIGHDLPSSVAT
                     NEQVACFFAEDQIYRIDHYLGKETVLNLLVLRFANSIFSTNWDNRVIDRVEISVAEEI
                     GVEGRWEYYDKSGQVRDMLQNHLLQILSLVAMEPPLSLSAESIRSEKLKVLKSLRFIN
                     KSNVHDHTVRAQYVANNLANGKKIPGYLEEVGANQASTTETFVAIKAYVDNWRWSGVP
                     FYMLTGKRLPKKQSEVVIYFKPQPYNIFHPLNEELYPNQLIIRLQPDEGVEVRIMNKI
                     PGLGERMMLHDSKLDLNFHHSFKTQRIADAYERLLLEVMLGNQYLFVSREEIEQAWQW
                     IDSIVSAWEEDNIPLYTYPACTWGPKEVIRLFNGQAHVWGDHNAVS"
     gene            15515..16228
                     /gene="pgl"
                     /locus_tag="U6309_000745"
     CDS             15515..16228
                     /gene="pgl"
                     /locus_tag="U6309_000745"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEN5504199.1"
                     /translation="MQFHSFRDAKQLIHDMVEKLRTILSDAIKQRGQAFLVVSGGRTP
                     IDLFKELALADLPWEKVVITLADERCVNLFDPHRNEWLVRKYLLQHNAKKAKFISLFD
                     EKEDPLSQLNEVNGQIDSLPTFDAVVLGMGDDGHTASLFPCSDELSAGLDDKANAVLI
                     VKPRTASFQRISLSKRRLLNSSTIFLHLVGQRKQAVLKQAMATNNPKILPICAILNAP
                     DVNVQVMYAPTDEEKECIL"
     gene            16216..18054
                     /locus_tag="U6309_000746"
     CDS             16216..18054
                     /locus_tag="U6309_000746"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEN5504200.1"
                     /translation="MHPVINQVTENIQNRSVARRAAYLNQIDKARTKGPQRSILHCGN
                     LAHGFAACAKQDKAILRGNAKANIAIVSAYNDMLSAHQPYSTFPDLIKEAISAAGGVA
                     QFAGGVPAMCDGITQGQPGMELSLLSRDVIALSTAVALSHNMFDGGLLLGICDKIVPG
                     LLMAALAFGHLPFIFVPAGPMPSGISNQEKARVRQLYAAGQVDKNALLDVEAASYHTA
                     GTCTFYGTANSNQLVIELMGLQLPGSSFVNPNTPLRNELTKAAACQILSLTDLNSDYM
                     PIGQMLDVKNIVNGIIGLLASGGSTNHTMHLVAIAAMAGYWVNWDDFAQLSAIIPLIA
                     RIYPNGQADINHFQRAGGMAYFVKTLLEAELLHPDVNTVVGFGLDKYTQQPQLIDNRV
                     VWVNGPDSSSNNDVLTSAADPFKPQGGLQVLSGNLGRAIIKTSSLYDEHYVIEAPAVV
                     CSSQEEFEHLFNQGLLNKNCVVVVRFQGPKACGMPELHQLTPKLGVLMDKGFHVALVT
                     DGRMSGASGKVPAAIHVTPEAVDGGGIAKIETGDMILIDCERGILQLLVSDEELAGRS
                     PVAIEGYSSVLGMGRELFGNLRLHFTGAEQGACSLFKGNEFFYDAS"
     gene            18029..19036
                     /gene="glk"
                     /locus_tag="U6309_000747"
     CDS             18029..19036
                     /gene="glk"
                     /locus_tag="U6309_000747"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEN5504201.1"
                     /translation="MNFFMMPHDLAQYAIVADIGGTFARFSRVNLVNLQMDKIEISPC
                     AEFISLESALLTYKNRHSLQEIKHIAIAIACPVIDDLVSMTNCHWQFSITELKQRLGL
                     EVLEVMNDFTAIAMSLPVLSTQDLVQIGNGYLDASKVRVVLGAGTGLGVAYLIPHQHH
                     YSAFAGEGGHADWGAKTEQEWFIYRYLKSKYSHVSYERLLSGQGLENLYQALAAYHSK
                     KVEFLSAAQIISLALNQECFIAHKAVAQFFSSLGSFAGDLALTYGAFGGVYIAGGIMP
                     RLLSLVHQSDFRIQFEDKGRFSDFNALIPTYVIAAAQPGILGASVSLKQILTGASHVL
                     F"
     gene            19023..19685
                     /locus_tag="U6309_000748"
     CDS             19023..19685
                     /locus_tag="U6309_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEN5504202.1"
                     /translation="MSFSEWKTQPGAVFAASPVIPVIVIKELEDALPLVEALFAGGIH
                     VLEVTLRTPVAIKALELLNNTFPDVLIGAGTVITPGQLHDVAAAGARFAISPGQTREL
                     LIAGQKSEIPLIPGVASVSELMEGLGMGYNHFKFFPAAAAGGIPMLQAISGVFPQVKF
                     CPTGGINSKNYEEYLRLPNVACVGGSWIVPEEAIKNHNWSLITELCMAVSSLKRELCS
                     QN"
     gene            19799..21220
                     /locus_tag="U6309_000749"
     CDS             19799..21220
                     /locus_tag="U6309_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEN5504203.1"
                     /translation="MAWIVAIIGSIAGFLFGYDEGIIAGSLGLVKNHFNLNATHIGVM
                     ASALPFGALFGALLIGAFMASKCVKRFGRRSLLSFAGFLFFVGALGAGFAETISVLIL
                     SRLILGLAIGMASVLTPLYLAETAAVQSRGAVVAIYQLALTVGIVCSYSVNYLLIEQQ
                     AWRAMFASSAIPALLLTLGILFMPESPRWLCSVGRHDAAANSLRKLRGKQSVEQELKD
                     IEATLANEPKQGNWLLLFQKPLLPVLMLGTILFCLQQLSGINVVIYFAPEIFKNLGLG
                     STTGQILATMGIGLVNLLVTIIAILYVDKLGRRKLLLLGFAGTSLSLFALSLFSLNHI
                     AWLSYLSVICLMVYIFSFAISVGPIPHIAMAEIFPLHVRGAGMGMSSMSNWSFNTIVI
                     FSFPVLHQMFGIDVTFVLYAVICFLGFIYAYIYMPETRNISLEQIETYIMSGKPLRFL
                     GREDEEVNAVSTKSESSLLASTN"
     gene            complement(21310..22659)
                     /gene="legY"
                     /locus_tag="U6309_000750"
     CDS             complement(21310..22659)
                     /gene="legY"
                     /locus_tag="U6309_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegY"
                     /protein_id="HEN5504204.1"
                     /translation="MATFFFKTNYYQEIMLKRIFFLMIFFVSQTMASVFTHEEVQILK
                     KHFLNNFQANGAIVASPSQYNPNYYYDWIRDSAIAMGLVETWYEASQSARYKKLLLEY
                     VSWTEKIQHQADPIAGQDILGEPKFYINGNPFDGEWGRPQNDGPALRASVLIRFAQQL
                     LDHNEIGYVKSHLYNNTMDPQSMGTIKMDLEYIAHHWQDANFDLWEEVYGHHFFTAMA
                     QQKALTDGAILAHQLHDRQAAVFYEMQANLINSRLQQHLDHQNKIIQATLLPHPGPQK
                     TLELDSSVMLGILTNPQKEGVFAPHHTFVRNTAKALHEQFNLMFPINKNRSGAILFGR
                     YPGDTYDGYQTNSIGNPWFILTATMAEYYFTMAHNLSPNSSNKLHIQNYLKKGDNYLR
                     LIKQYGPDLNLSEQINLNTGVQQGATSLTWSYVSVLRAIHLREQLENRIKTMGWNTY"
     gene            complement(22785..23021)
                     /locus_tag="U6309_000751"
     CDS             complement(22785..23021)
                     /locus_tag="U6309_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN5504205.1"
                     /translation="MSIIVNLDVMMAKRKCRLRDLAEAIGITEANLSILKNGKAKAIR
                     FATLEAICAYLHCQPGDILEYQSIEDNHLIKDRA"
     gene            complement(23108..23647)
                     /locus_tag="U6309_000752"
     CDS             complement(23108..23647)
                     /locus_tag="U6309_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEN5504206.1"
                     /translation="MKKIKSVSHLLFLLFKTLAYGLPLVTTYFILFHFESMLNWGGWH
                     SLLSTPVKDPSHFSLLHRFIILLIEALPLSINVLICHQLAKLFRLYEHGYLFEEENIR
                     LIKNISIYLISGELIQLLYQPLITAALTFTNPSGQRIASITLGTANLSTLITAFIILI
                     ASWIIKEAHKLKTDALLTI"
     gene            complement(23775..24770)
                     /gene="hemH"
                     /locus_tag="U6309_000753"
     CDS             complement(23775..24770)
                     /gene="hemH"
                     /locus_tag="U6309_000753"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEN5504207.1"
                     /translation="MRRGLLLLNLGTPDNADIRAVKLYLREFLTDKRVIDLPTIPRYI
                     LVYCLILPFRSPKSAQAYQSIWTEKGSPLLYHSQNLVTKLQSALKDEYKIALGMRYGT
                     PSITTALAELKDCHSLTILPLFPQYSSAATGSAIEKTLSYLANQEIIPSIKIIRDFYQ
                     RPEYIQAQAKIMKPYIKDNFHVLFSYHGIPERHIHKSGCDTLCHQTCTPIYDKNQACY
                     RAQCYQTSLLLAKELQLGTHQYTTAFQSRLGKTPWIKPYTDEIFAELISKGIKNIVVS
                     CPSFVADCLETLEEIGIRAKEQWEKLGGEQFILTPCMNDHPEWIKAIQSIVNEQF"
     gene            complement(24881..25114)
                     /locus_tag="U6309_000754"
     CDS             complement(24881..25114)
                     /locus_tag="U6309_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN5504208.1"
                     /translation="MARGEVKWFNNAKGWGFIIPEGGGEDIFVHFSSIHGTGYKTLVP
                     GQAVTYDVVNGERGLHASNVVALSENAEQEEIA"
     gene            25351..25764
                     /locus_tag="U6309_000755"
     CDS             25351..25764
                     /locus_tag="U6309_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEN5504209.1"
                     /translation="MFCIDNQIKSSSFILGEWPLSTVLLKNDASYPWFILVPRKMDIQ
                     EIYQLEKSDRFLLIEEINQLSLLVRDYFKPDKLNIGALGNIVSQLHIHIVVRRKNDPL
                     WPQGIWQSSMLSIPYPESELKSLLPTLGDLVNKAY"
     gene            complement(25867..26226)
                     /locus_tag="U6309_000756"
     CDS             complement(25867..26226)
                     /locus_tag="U6309_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN5504210.1"
                     /translation="MIKKIAFTMYPVQNMDRARQFYEKTLGLKVGKISAEGAWVEYDL
                     PQGGCFAITTLAQGVIPSANSGGSVAFEVDNLDDLVFQLKSKGVQFKIDLFASPVCRM
                     AIAVDSEGNAFTLHQLT"
     gene            26322..27665
                     /locus_tag="U6309_000757"
     CDS             26322..27665
                     /locus_tag="U6309_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEN5504211.1"
                     /translation="MLKKLMFLLLLGAGLLSCGQSTDQQISNIITPKQFPSSPRDYRP
                     VQELPYLAWWKQFQDPELDRLIDTGLKTNMDIHIAMANLQQAQGELQQVKLSWIPNVQ
                     ILGGYSTNPALGVPGGFYGIWPSYILNIMQLYTQQKRAQYQVQYHQAAIDGLRLTLIG
                     QVAAAYFTLIAQLEQLKLLYELDRDLKSLIALSRKEINIGLKNDIDLAQLRSNERLIA
                     AQIKPIKHNIVVSQNALRYLINENPGLIKSKNNFAQLDFTRFKPGSLPATVLGNRPDM
                     KMAEYSLKAARAGIFVAYSEFFPVMQLDDFIGEAHLPDSTFEQATDAYVNWTINPNTL
                     GKISVSKGVYNAKLAEYVKTVRLILKEVDNAFSANKRMREQFLSYRHAQEDYQHKYKL
                     QNGLLKSGLMSYKELLESKVYLDTLALSTNQAKLELAMSLVMLYQDLAGGYACSD"
     gene            27804..28841
                     /locus_tag="U6309_000758"
     CDS             27804..28841
                     /locus_tag="U6309_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEN5504212.1"
                     /translation="MKKLMEYGGKIKEYLLRLKPKRWFTLTNVIIFIGFLTAIIYVFS
                     YLIPFTDNAFVVNNVRPVAALTNGYITELYVKNGDAVHKGQKLFTVFKKPYEYTVEQL
                     SADLAGAQAKLAVLKATYERDLKLSENEQKIYKKLAQDDQKYLKGYAIKSVSLITLQN
                     SQQETKAAKDKWQASLKQLEIDQHQITVQENEIKSIQARLKNAKVNLDLTDVYAQGNG
                     VIQNLFFTIGTPVNINQPLFSLVDQDNIYIQANFNETDLRDVRKGSKVLIFPRMYLGR
                     KIFHGVIDSDYWSANRQLVDDRTQLQNVINENQWILLPQRLPVIIKVTDPDPKYPLRV
                     GASAYVYVEVY"
     gene            28819..29751
                     /locus_tag="U6309_000759"
     CDS             28819..29751
                     /locus_tag="U6309_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504213.1"
                     /translation="MSMSKFIEWIDEVDPYAVQRIALYKSLFIATVMAYVYWVFRPTN
                     FTAFFSPFLLVRFYESPSLTSFKEKEHFLIFIGVVVVLLSTSFYLVYPFRVVFFFFSI
                     IALASVYFYVLKNYLPLRNVTMLIIASGATILSTEPPANWQIVYDIVGSSALSMIAIF
                     ICLRFFPNQYLSVWKRALAKFIQSLELDIEGSFTHRGPKFMQEEMTHLGMLRQYRRLI
                     PQKYMIYTQRISINIRNIQFSLDNLYYEAKNEAFWHGVKENLYTLRCAIKTNTSCGTP
                     KMSIMPESKLQQYVMRCLQQAFSQWNQLCMILQH"
     gene            29718..>30260
                     /locus_tag="U6309_000760"
     CDS             29718..>30260
                     /locus_tag="U6309_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN5504214.1"
                     /translation="MESIMHDTSALKKKLDYARFIHLVIMFMVAMLIFLFSTFPEKWW
                     VLITVLSVSAGIEPGLIIRRAKHRIGGTLLALIILIPLLYLLQLNYRLISILFVLAIV
                     GLSVATLNTRRYDISVFFITLVVFFLMAQTEEATSPRGPFEMVLNRGICTLFGVFIVL
                     AGDYFLFQAYRYSHRLYFFHQ"
BASE COUNT         8835 a         5271 c         6381 g         9773 t
ORIGIN      
        1 atatagaata agccattgat tttaaaattt ttttataaat tctcataaaa aacacttcaa
       61 ttaaaaatac attattccta ttttttaagg tttattttca acaagcattt atctcatctc
      121 tttatatttg ccaataaaaa atcaaattgc ttatttttct taatattttc ttaatgattt
      181 ttagttatta ttttgcacag tttgtatctg gatgtggtga aagttatggc taaaataatc
      241 ttggtgtatg ctaattgtgt taattcaact gcaaaaggcg attttgctct ggctggacag
      301 attgccaaag atttagttag agaaaaaact tctgatgata tcgatgtggt cttaacctct
      361 actttggatg gaattgcaaa atttgaaagc ctttatggca aagcagttga tggccgtgta
      421 atgattgaag ggaccagcgt aggcttatgt gccttggaat tatttgactc ggtagataat
      481 gaagtggtgg ctttcattga agcaaaccga tgcaaatacg cgccaagtga tattctgaag
      541 cgagttctct ctcctgacag caaattttta tttattggag ctgctaacca aaaggcaata
      601 gcaaaaggcg ataaatttac taaatcctgg ttgtacataa gccataaagg tgatcagcct
      661 aatgtttatg agcattttga cgaagacgat tctctaatac aaagtgtagg tttaggtgat
      721 gatcgattag gattaccatc cttgccaaaa gtcgatgaat taccggagat gaactccaca
      781 caatcacaaa aaataccaag cggcgagtat ggatttatgt atcttgccgc tgtacatggg
      841 atagatgacg tttatttgat gaatcaatac atgtctttaa ctgagatggg gcaatatgtt
      901 ttggtgggtg aatacgccaa acaatcaaaa gaagttcaaa tggttctgca tagtctacag
      961 tataaaggta gttcggtatc actagcgatg cctcagattt tcttccatga ttcggtcgat
     1021 aattgtttaa tgcgaaacat ggtttcaaag tcttctggtc ctttggttgt ttctacaggg
     1081 gttatgagtt ctattgaggc gatgcaagat ggtaaattaa cgtattatca gacaatgtct
     1141 aataacacgc aattcgtggc ttcttatttg attgctgtga aatccatttg ctccagtgat
     1201 acaacgttat ttgggtctat gccgaaatta attatcgatt tgtccaattt gctttttgca
     1261 gaaaaaccat tgaaagaaag ccaagtaaaa gagattagaa ccttactggg catgtcttcg
     1321 gttaccagcc ggttgggtga aatgaataaa agaatcatag ctaaagcaaa cgggactgtt
     1381 gctggtgagc ttttaagctt tattggaaaa cctaaaacga cacaatcaca taaacaatgt
     1441 attagcgttt gcatgtccct tcgcaagagt ggtgaaacta cgtcacctat ttttgatcaa
     1501 gcattacgca gagcagcagc ttggggtcgc ttgtttgaat tgaaggtgtt gattgcatca
     1561 atgtcaactt ctgatttaaa taaaaaagat aaagcaggta atcaatacac agcacttcat
     1621 tgggctgtga tgcaggggca cttagattgc gcaagattat tagttaaatc tggcgcttct
     1681 gttgatattc aagataagtc tggtaaatca cctttacatt atgcgattaa aagaggagat
     1741 aaagaaacca ttaaattatt ggttcaagca ggagcttctt tggaaattat ggatgattca
     1801 ggagcaaaac cttgtgatgg atcagagtct tgggttcctg agtatattaa atcctgttat
     1861 gagaaaagtg gccacagtaa atttattagc ttattttaaa ttagtcttaa tgacctgttt
     1921 agtaattatc tcaataaccc ggatatgaaa tttacagtcc gggttatttt tttattgaag
     1981 tatttgggta atgctgtttg tttttctggt attaccaagt tttttgaata aaatttaatc
     2041 ctgacttccc ctcgacttat tttatgggtt aattttatac tgaattcatt atggatagca
     2101 tcaggttcaa aggatatgat ttatgtttta atttgtatta tgctagtttg cggtttggtc
     2161 gtgtatcaac tcatgcccaa gcttctttcg tattctactt ctttgatctc caaaaaacag
     2221 gacaccagtg aattaacaca tgatggtatt acgaatgtct taacacaatt aggtcacccc
     2281 aagtttgaag gagtctgtta tggcttcact ctcaattggg cattggcagt ggctcagggt
     2341 aaagaatcct ttttttaccg ccaattgcat tatttacgaa cccaccagtt tgatttgcct
     2401 gaaacattac aacaagttaa agaaaagaaa gaaaggaatc aatcattaag caaagatgag
     2461 gtggtcattg aaactttgcc tcagttagga aagaaaattt gtgttgctca agaccccttg
     2521 caatataaag agaaatacag aaaactggta tggcagcctg atattaattc cattttgaaa
     2581 gccataaatg ccgattcctc cgttgcaaag cacatttttt ataagactca tagtttttta
     2641 aatcaggatg aagcaacaga gtatcttgaa ttattaaaaa gaacaggcat cagagaggat
     2701 gttgcagtaa ttatcagtac cgcagatcac gccatgggat ttaaactggc tggtaatgtc
     2761 tggcggttta ttaacatcaa tgatttatac caacaagaca aaaataaacc ttactttgag
     2821 ttctcgtccc gaaacctggt taaagagttg tatcgtgttt gtgccgaaaa ccttcaggga
     2881 agtcgattga ccgtgaatac cgattttgtt tcagttaatc ctgaagaaaa actgtcaaga
     2941 gcactgcaga atgtattccc tgtgtttcca gtcagaacca aaacttctta tcctgaacga
     3001 ttggcttttt tttccatggc agctactcag ggtgatatga attctgtaaa aaaatgcatt
     3061 cattctggct ggtcaatttt ttctcgacaa cgactaagtg atgattcgcc tattcttacc
     3121 gccatctatt tgggaagaag agacgtagta cgtgccatgc tgagtacttc tcgacataga
     3181 gttaaccaaa agcgtaaaag cgattcttct actttgctcc atatcgcctg tcggtatggt
     3241 ggtagtggta tagtcgaaga tctgttgaat atacgtggaa ttaaaatcga tccccaagac
     3301 agcaagggga ggacgccgct catgtacgcc tgtaaaaaat ctgtagtgac ggaggataaa
     3361 aaattattta atttgttgtt tgcaaaaggc gcttctcttt ctattaaaga taatgacggt
     3421 ttgaccgcac ttgatcatgc tcttaaaaat gagcataccc ttgcaatcca aatgatcgag
     3481 gagagattgg aaaaagaagc ttgtgcgcgg gagaactcaa cctctcgtcg atttaaattt
     3541 tctgagacaa aaggtacttt gttccagaga ggagtgaaaa ctatttctta tcaatcacag
     3601 caacctggtt ttggtatgaa ataggagaat aaaatttgtc tttttagcac ttcaagcttt
     3661 ttgtctcgat tttggggttt tgtgccctat tagtaaacaa accacttaat gtgcattaga
     3721 aagattcttg ttattaattt tatatacaga atactggttt ttaatgacat aacatcatat
     3781 gatgttttcg catcggacct gtactgaaat gaaaattctc aatgcctgtt gggtcaattt
     3841 tgtaggtcag tatactaagg ggtatatttg gttatttttt attcgattat taaggtttcc
     3901 ttaagcttat ttctttataa taaacttttg attccaaata gtcctcactg aaggaataca
     3961 atgagcaata aaaaacattc tctcactata tttagcttga ctatgattac cgttggctct
     4021 gtagacagta tccgtaacct gccagctact gcattatttg ggagtcaatt gattttttat
     4081 tttatcctgg gtgccttatt cttcctcatc ccaaccgcat tggtctcggc agagttagct
     4141 tctgggtggg ccaggcaagg tggtgtttat atctgggtta aggaagcttt tgggaaaaaa
     4201 tcagggtttt tagccatttg gttgcaatgg atagaaaatg tgatttggta tcccacgatt
     4261 ctatcgtttg ttgcgggaac aataggttat cttattaacc cctctttaac taccaatcct
     4321 tattttcttt gggcagtcat tgtcagttgc ttttggggag caaccattgt gaatttgcgc
     4381 ggtatgaaat catcggccat gttcagtaat atttgttctt tggcaggatt attattaccc
     4441 atgtctttga tcattggttt gggtgctgtt tggatagctc aaggtaaccc cttgcaaatc
     4501 aattttgaca tgcccagtat tgttccccat ttatcggata agggcatgtg ggtttcatta
     4561 accgccatca tattgtcgtt ttgcgggata gagattgcta cggttcatgc taatgatgtt
     4621 aaggatcccc agcatgcttt ccccaaagcc ctggtttatt ctgtagggat cattttgagt
     4681 actttgattt taggttcatt agcgattgcg gtagtgctcc cacaaaagga catcaatctg
     4741 gttgctggaa ttatgcaagc cttttctgcc tttttcctta aatatcacat ggattggatg
     4801 atgcctgtgg tagctgtgat gttggttctc ggagggcttg gtggtgtgag taattggatt
     4861 attgccccta ccaaaggttt attagttgcc gcagaagaag ggaatttacc agaggtattt
     4921 caacggacta atggaaaagg tgctccggcc atgatgctat acacccaggc aaccatagtc
     4981 acagtattgt cggcgttatt cttatttatg cccagtgtca atggatccta ttggctctta
     5041 acggcattgg cggctcaatt atacatgctg atgtatttga tcatgtttgt cgcagcaatt
     5101 aaattgcgca taagccaacc ttaccatccc agacctttta aaattccagg aggtatggca
     5161 ggaatgttgt ttgttgcagg tataggtatc attggagtgg ttaccacttt agcggttagt
     5221 tttattcctc cagaggggat taatgtcggc agtgcaacac gttatgaatt gactttgatt
     5281 attggtttgc tgctcatgtg tgctcctcct tttataacca gttggttgca atccaaagag
     5341 gcggatttgg aacctgctgt ataatttaac aagacaggtt aattttaata accccagttg
     5401 tttatgtaac tttcaaaata tgggctatat tgacgaattg ttggaagaac tatctgcacg
     5461 attgccagag ctggaatgga agatcagtgg attgagttct tctatttcaa ttcataattt
     5521 gcccaaaggc atattttctt ccgttataga attcaatgga cctgcctgta tcaaggaaat
     5581 tcaggatgat attcacgctt tacagaaaca taaggatgag tcaattgcat gctttttggc
     5641 agagcgtatt cagaaaaaaa ttaatgtact ggtcatatta tgccaaatgg acaaaaagaa
     5701 taataaacct gaatcaaaaa tttcttttga tttgacgact cttagtacaa gacaacaatg
     5761 gattaaaacc atggaagaga agatctgtgc cttggaggaa caatatcaag ccatgagaaa
     5821 aactttagag caaatgaagt cctgttctaa tcctgcaact attttgcatt tacaaaccga
     5881 attagggaat gttgagaaaa gacttactct ggctaaggaa acgctcaatc gagcaatatc
     5941 ctgaaatcgc cacttcttaa attaacgtga gttatcgata gggattgtat aagaatcagg
     6001 cgatatcctc agcctggacg attcaagata acgacctatc ttatacatta ctcacgttaa
     6061 attagggaat acatcttttg catccctata ttatgctgta aattagggac gcttgaaaaa
     6121 atataataag gaaaggagta gagcctcatg tcagacaaat tttcacacat tacaaaagat
     6181 atcactacgc aattagccaa gtttcgcaaa gagatgccag agttaatgac tggattttct
     6241 tctttggcac aagctgcgac gaaagacggt gcactagata aaaaaaccaa ggaattaatc
     6301 gctatggcgt tggccgtggc caagcaatgc cccggatgca tagggtttca ttcacaaact
     6361 ttagtgaaac tgcaagcgac aagagaagag ttactggaaa cattaggtat ggcggtttat
     6421 atgggaggcg gcccttctct aatgtatgct gcagaagcat tggaagcttt tgaggaattt
     6481 agtaagtaat tataaagaag ctaaagttgt gcttaataaa cactaaaatc aataaaaaac
     6541 cagcaaggca gagtttttgc aaacattggc aagggaattc acagtgtttg acgctctgcc
     6601 aactttgctt tgccatgccc actctaacaa ttttcagaac atgtcgtctg gtttaaagaa
     6661 acggatgtag aaaaaaaatt ccgggacttt gttgctgagg gcgctattgt ggcgataaca
     6721 gggaagccac atgttgaatt tgtttctcct gaggactggg ttcacaaggg tattttttgc
     6781 taagccctaa taaagccact tgagaaacgg tcattttatc tttgtcgctt tgttgactgg
     6841 atatttcttt ataggtttgt tgtatgagct cgttcattac aaaagaatta agttgcacac
     6901 cttgtggtaa gcaaaagata ggtttacctt gtgtttttgc ggcttcattc gcctggatta
     6961 aagtttcagc aatactttca ctggtgatgg taagtacatg acgtgccgat ctggcccatg
     7021 cttgagcttg agggtctgct ttcatttcca tttgcggaat attgttagca atattcatat
     7081 aattatttat tgtatcggcc tgtaaatatc cgctggcaag aattaaactg ataaacaaaa
     7141 gacgcatttt aaatcctcaa gatccttaat tattatttag taaataatat ttgctcgggt
     7201 tatctggaca ttcgtgcggt ccacattcca agaatgtact gattccattg gcaagaatca
     7261 agataaaaaa tacccatatc attacttttg ctgacttgct tcgtacttta taatctggca
     7321 tgaggccctt atcaaggatt agagccccag caattaataa gataatgact aaaaatatga
     7381 ttgctgacca agtgtaaaga tgcaaaccca aaaagaggcc gccataacca ggatcaccag
     7441 gtgttaaatg aatatacatt tgccgaaccg agatagacag ccctaaaaga cttgccaaaa
     7501 gcatcagccc gtaatgtgaa gctctgatac ccagcattaa attcataaca aaacagaggc
     7561 ctactgcaac aaagcaaatt ctttgcaaaa tgcatatagg gcagggtagt tcataaaaga
     7621 tgaattgatc caggaaggcc aagatcaaaa ggcaacaaat tatcaataac cccgaagtat
     7681 tcccgaaaag atgccattgc atttgtttat tcataggttc accagatggc tgtgataatt
     7741 gaagctgatt agtataagaa acaaactgaa aagaaattgg agtattatcg ttttattggg
     7801 tttaaatata acttgtaatc ctattatgaa ataaaataaa aacagaacta tcatcatgat
     7861 gctcaccatc ccttttgagg gttacaacaa aagtatagat agtatattga gcaatttcga
     7921 aattttattg taagagagta gcccctcctg cctggaacgg tggtattggt ttatggtcaa
     7981 ttgatgctcg ctgcgagatt ttttctcgtt gtttgtcatc tcgtggatta gggggaattt
     8041 cctgaaggtg gctgaaatga ctatttaaaa gtatctcatt atctcagcta tgaccctatc
     8101 tcttgaggtg aatttattaa aagccaaaag ctaaattcca tgtggctgaa tcaatcccaa
     8161 tttgaaggca ataaataaaa acacaaataa agataatgaa attgcaatgc gcacggtgag
     8221 cgctttgact gtgcgctttg atttatcagt gtccttgacc aggtaaaaga gcccgctcgc
     8281 taatgaggcc acaataagaa tcatgacaaa gatgatgaag attttagtga acatttgtta
     8341 tactcctgac attgctagct tcaggggaat gttagctaac caaatgagta gttgataaaa
     8401 gatgcctagt ttaacctgtt ttaattttcg tttcacctca aattggccaa tgcttattct
     8461 tacggccgtt tgcttttttt tattcattag ccttggattt tggcaaatac acagagccga
     8521 tgaaaaaact gaaatgataa ccgctcaaca agcgctggca aagcaggagc ccataacttg
     8581 gcagccaggg caaaaattgc cagagcaata tcaacgaatc agcattgaag gagcttttct
     8641 gccaaagtta tttttgcttg ataatcaaca ttatcaacat cagtttggtt atgacgttgt
     8701 ttctcctatg cttcttgatg atgactccat cgttatggtg gatagagggt gggtttctgg
     8761 agacattact cgacgcactt ttcctaatgt tcagactccc aacggcaaat ttaaactatt
     8821 tggcatggtt tattttccaa gtaaaaaaca atgggtatta gggccaagtt ttgaagaaaa
     8881 agagaataag gtgactcttc ttgagctcat tgatgaagaa atactgaaac aacttttgca
     8941 aaaaaaggtc tatccgttta ttattcgcct cgacaagaat gagccttttg gttttgtacg
     9001 tgaatgggag atagtatcca tgcccccgca aaggcacttt gcttatgcaa tacagtggtt
     9061 tgccatggca ttggttattc tgattatatt cgtgattttt aatttgaaga aaaatgaaaa
     9121 aaccatataa atacatgaca ttactactct tggtagtgat ttttgctgca ccaggaataa
     9181 ccgcttattt gttctataaa aatccttcct ggcttggaac ctctaaagta aataaaggaa
     9241 ttttactttc tccaccgatc gctctaaaac cattagatag cagtgcaaaa tggcgcatta
     9301 tctattggaa tcctaatggt tgtgacaaga cttgtcttaa gcagcttaat atgttgggaa
     9361 gagttcgtct ttcttttggg agaaaattat atcaggtaga tgaatggttg cttctaagca
     9421 acaaagattc attgatcgca gaggaattaa aacccatttt aaaagaacaa gatatccatg
     9481 ttgctgcctt atcattaact gaaatgtcta aattgtcgac tttatatcct gaagctaaaa
     9541 tttttatagc aaacccggat aattacctca tactgagcta tcaaccaggg tgtaacccag
     9601 gtgatatata taaagattta aaacttttac tcaatacaac tgaaattaag agcggataaa
     9661 ttgatgtcta atagttcaat acgattagca gcgactcttg ctgttttttt ggcttttttt
     9721 gtcgttatgt taggcgctta tactcgttta actgatgcgg gcttaggctg cccggattgg
     9781 ccaggttgtt atggtcgcat ggttcttcca ggagcagata aaggtttgca agaagctcaa
     9841 tcacaatacc cggaccttcc tattgaatcc aagaaagcct ggaccgaaat ggctcatcgc
     9901 tatgcggcag gaacgttagc cctgttgatt tttttcattg ctttccaggt atggttcaaa
     9961 cgtcgtcagg atcaagattt gccttggcat cttcccttgg ctttatttat gcttgtggta
    10021 tttcaggcgg cattgggaat gtggactgtt actttaaaat tactgcctgt agttgtcatg
    10081 ggacatttgc taggaggcat gttaatcgtt gcctgtttat gccgttttcg tttgcagata
    10141 gcccagctaa agggtcaaaa tttacctgaa tggcgaccgt ggttgcgttt gggaattgtt
    10201 atcattttgt tgcaaattgc tttgggtggt tgggtaagtg ccaattatgc aggtatttcc
    10261 tgtattggtt ttccattatg taacggaacc tggtttcctg aattgcattt tgcaaaagga
    10321 tttaatttat tttctacgat aggcgccaat taccaaggcg gtgttcttga gagtgaagtt
    10381 agggccagca ttcaatttat tcatagaata ggggctatat tgactgccac ttatctgctg
    10441 actctgtcat taataatcgt attcaaaacg cattcaattt atttaagagc aactgctatt
    10501 atcatggcta tgttagtatt acttcaattc actctaggga taatgaatgt gattcatcta
    10561 ttaccactca aggttgcggt cgctcataat ggtgtagccg cattattatt agcggtggca
    10621 ttttgttctt tgcattttac acaagataag gaggttagcc atgcgtactg aatacgcagc
    10681 tcgacttcct gtggattggc gtgattacgt ggaattgtgt aagccacgcg tcgtgttgct
    10741 catgctactc actgtcattg taggaatgta tttagcagct cctggctggg tgagcttgcg
    10801 gttaatcgct tttactttgc ttggtatagg actgtgcgct ggcagcgctg ctgcaattaa
    10861 tcatttggta gacaggcata ttgattcgat tatggccaga acaaaaaaga gaccagtcgc
    10921 ttatggacgt gtgtcggtaa agcaagcctt atggttcgcg gtcattatcg ggctaatggg
    10981 attaagtctt cttatcctgt ttgtgaacca attaacagca ttacttacct ttgtaacact
    11041 gattggttat gctggtgttt acactgggta tttaaaacga gccacttcgc agaatattgt
    11101 tatcgggggc ctagccggag ctgcgccccc cttattaggc tggactgcgg ttactggcca
    11161 attggatcct caggccttgt tactagtatt gattatattt acatggacac ctcctcattt
    11221 ttgggctttg gcaatttatc gatataaaga atatcaagat gctgaaattc ctatgttacc
    11281 tgtgacacac ggtattcaat tcactaaatt aaatatttat ctctatacgg ttttattgct
    11341 ggtagtcagt tttttgcctt ttgttgtaag tatgagtggc tggatttatt tgctaggtgc
    11401 tttggctttg ggcatcaggt ttttggtttg ggcacataag ttgtatttta ctgataaacc
    11461 agtcgttgct atgcaaacat tccgattttc tattctgtac ctaatgctgt tgtttgtctt
    11521 tttattagta gatcactatt tttaaggggt tagaatgaac ataaaaataa aagggggcgc
    11581 tgtaacaatt actgtattgg ccctggtcag tttattggct ggtatttata tctctcagaa
    11641 ttttcatttt aaaaagaaga tcaatgtggc ggattttcac gggacttatc ttgaaaatcc
    11701 aagaacagtc aataatttta gcttaacagg cacagatcag aaggctttca ataactcaag
    11761 cttgcagggg cgctggactt tagtattttt tggatttacc aattgtggtt atctatgccc
    11821 gactaccatg gcagagcttg gaaaaatgta tcgaattctg gaaaagaaag gggtgaaaaa
    11881 tttacctcaa gttgtgatga tatctattga tccgcaaaga gacagtcttg ataaattggg
    11941 tagttatgtc acttctttcc ataaatcatt ttatggtgca agaggagaag aggattcaat
    12001 taaatcaatg acccatgaaa tgggcattgc ttacgcaaaa ataaacaata aagagagttc
    12061 ggagcctgaa aattatgata tacaacatag tggggctgta atgctattta atcctcaagg
    12121 tgagttgaat gcatttttta caacaccaca tcatgcggat ctcttagcta aggattatgt
    12181 cttattggta tcttaacctg taattcgata ttttacctct gcaggagcaa tggctaagca
    12241 gacttgtttt atttatgtca aagaaatcta tctagaatgc attagttaat taattgactg
    12301 ttgcatgaat tcaaaaatta ctctcctgtt gggtacggca gggaggataa tttgtgattt
    12361 ttaatctagg ctttaagtaa ggattgttga catttcattt atcttggctt aaaacccgcg
    12421 attttctacg cttggctgct tttatcattt cagagcagct ctgttgtgat gcttaaaact
    12481 tgcgttattt aatagaaaat tagggaaaat ggtttggatt gttcagattg aagtatgaaa
    12541 ttactgagga aagtttaatt catgaaaatt gcgatattgg caactaaccc ccatttgtat
    12601 tctcataagc gtttaaaggc agaggctgag gctgcggggc atgaggttaa aattattaac
    12661 cctttatatt gttatatgaa cgtggctgct tctaatccta aggtacatta tcggggtggg
    12721 gcgcctttac cgcattttga tgccgttatt cctaggattg gcgcatccat tacctattat
    12781 ggtacggcag tattgcgtca tatggaaaca atgggcatgt acactcttaa tgaatccata
    12841 gctatatcac gatccaggga taaatttcgc tctttacaat tgctggcacg taaaggaatt
    12901 cctatgccgt taaccagttt tgcccagtct cctgatgata ctgaagattt gattcatatg
    12961 gttggtgggg ctcctctggt cataaaatta ttagaaggaa cacaaggcaa aggagtcatt
    13021 cttgctgaca gtcatcaatc agctgtgagt atcatcaatg cttttaaaga aatgcatgcc
    13081 aatattttgg tccaggaatt cattgaagaa tcccgaggta cggatatacg ttgttttgtc
    13141 ataggagaaa aagtagttgc cgcagttaaa cggcaggcca aggatgggga gtttcgcgca
    13201 aatgtccatc aaggaggaaa ggcagtaaaa gtcaaactct ccccacaaga acgggcaatc
    13261 gctgttagtg ccgcaaaaac aatgggatta agagttgccg gagtagatct gattcgctca
    13321 aatcatggtc cattggtgct agaaattaac tcttctccag gtcttgaagg tatagaaaaa
    13381 gcaactaata ttaatttggc aggaaaaatt attgaataca ttgagaaaaa agcgaagcca
    13441 attagttcca atcatagatt tcatggataa tttctaatgg gctcctgatt tggagtcact
    13501 tttgccaatt agccaatccg cattgacttc cagttcctga gctaatgttt ttaatacggc
    13561 ttcatcaggg ctcgtaattc cattaagcaa agcttctgct ttaaattttg gaattttgat
    13621 gagtttggaa agtatctcaa ttcgctctgc tgtggcctcc gggactccaa tattatccaa
    13681 ttctttattc agtcgttctg aaaatctctt gttaagcatg agtggctcct gatcaaaggg
    13741 attttttact aacgtgtact tatttcatat agcataaaac cgaagttttg caatttttag
    13801 acaaaaatcg ctaattttga taatgttgta gttcataaag cggatttcca tgtagatttt
    13861 ttaatccttt tttatcctct accgctttta attcacttca attgatttga atgattaagc
    13921 aggtaatgta tctggtagaa tgagcgaaaa aaatcaattt tgattgacgc ttgattccag
    13981 tgatgtaaaa tcgatgcaat ttcagggaca tagttatttg taattttggg atgatatatg
    14041 ctcttaaaag cagaatcgaa gtatccttgt gatttaattt tgtttggtac gcttggtgac
    14101 ttgtcttgtc gaaaattgtt gcccgcatta tatcaattag aactggctaa tttacttcac
    14161 gaaaaaacaa gaattattgg gtgtgcccga gaagagctcg atttttctgc ttatgttgac
    14221 ttgattaaaa ataaaattca gcaattttta tttgatgaaa ttgatgaagc agtctggtta
    14281 cgttttataa agaaactggc ttattgcaag cttgatctga atcaatcaga tgattatcaa
    14341 aaattaacgg actatgttaa cccttctgag cgagtggtta tttcttactt ggcaattcct
    14401 ccaagccttt attctcaaac ttgccgcagt cttgcacgcc ttaatctaac acagaaacca
    14461 tcgcgagttg ttttggaaaa acctattggc catgatttgc cttcttctgt agcaaccaac
    14521 gaacaagtgg cttgtttttt tgctgaagat cagatttatc gaattgatca ttacttgggt
    14581 aaagaaaccg tcttgaattt attagtgctt cgctttgcta attccatatt ttcaaccaat
    14641 tgggataatc gcgttattga tcgagttgaa atttctgttg ccgaagaaat cggtgttgaa
    14701 ggccgttggg aatattatga taagtcgggc caagtcaggg acatgctgca aaatcatttg
    14761 ttacaaatat tatctttagt tgcgatggaa ccccccttaa gcctttcagc agaaagtatt
    14821 agaagcgaaa aattaaaagt actgaaatct ttgcgtttta ttaacaagtc caatgttcat
    14881 gatcatacag tacgagctca atatgtggct aataatttag caaatggtaa aaaaatacct
    14941 ggttatttag aagaggtggg tgctaatcag gcatctacaa cagaaacttt tgttgctatc
    15001 aaagcctatg ttgacaattg gcgatggtct ggtgttccat tctatatgct tactggcaaa
    15061 agattaccca aaaagcaaag cgaagtggtt atttatttta aaccacagcc ttataatatt
    15121 tttcatccct taaatgaaga attgtaccct aatcagttaa ttattcgctt acaacccgat
    15181 gaaggggtgg aggtgaggat aatgaataaa attccgggtc ttggtgagcg tatgatgttg
    15241 catgattcca agttggattt aaatttccat catagtttta aaacgcaaag gattgctgat
    15301 gcctatgaga gattattgct agaggtcatg ttaggtaatc agtatctttt tgtaagccga
    15361 gaagaaattg aacaggcttg gcaatggatt gacagtattg tatcggcatg ggaagaagat
    15421 aatattccac tgtacaccta tccagcttgt acctgggggc caaaggaagt catacgctta
    15481 tttaacgggc aagcacatgt ttggggtgat cacaatgcag tttcatagtt ttagggatgc
    15541 caaacagttg attcatgaca tggtggaaaa attaagaact attctatcag atgcaatcaa
    15601 acaaagaggg caggcgtttt tggtggtttc cggcggtaga acgcctatcg atttgttcaa
    15661 ggaattagct ttagccgatt tgccttggga aaaagtagtg attactttgg ccgatgaacg
    15721 ttgtgtcaac ctttttgatc cacatcgtaa cgagtggctg gtgcgtaaat atttgctgca
    15781 acataacgca aaaaaagcca aatttatcag cttgtttgat gaaaaagagg atccattaag
    15841 tcagttgaac gaggtgaatg gccaaattga ttctctacca acgtttgatg cagtagtttt
    15901 aggaatgggt gacgatggcc ataccgcttc tttatttcca tgcagtgatg agttaagtgc
    15961 aggattagat gataaagcaa atgcggtgct tattgtgaag cctagaacag cttcattcca
    16021 gaggattagc ttatcgaaac ggcgattatt aaatagcagt actatttttt tacatttagt
    16081 aggtcaaaga aaacaggctg tcctgaagca ggcaatggca actaataacc ccaaaatcct
    16141 gccaatctgc gctattttaa atgcaccgga tgtcaatgtt caggtgatgt atgctccaac
    16201 tgatgaggaa aaagaatgca tcctgtaata aatcaagtaa cagaaaatat ccaaaatcgt
    16261 agcgttgcta gaagagcagc ttatctgaat caaatcgata aggctcgtac taaaggccca
    16321 caacgaagta ttctgcattg tggaaatttg gcacatggtt ttgctgcttg tgctaaacaa
    16381 gacaaggcta ttttgagagg taatgcaaaa gccaatattg ctattgtttc tgcttataat
    16441 gatatgttat cagcgcatca accgtactct acttttcctg atttgattaa ggaagcgatc
    16501 tctgctgcag gaggagttgc gcaatttgcc gggggggtcc ccgccatgtg cgatgggatt
    16561 actcagggac aaccagggat ggaattaagt ttactcagcc gggatgtgat cgctttatcg
    16621 actgctgttg ctttgtcaca taacatgttt gatggagggc tcctcttagg tatttgcgat
    16681 aaaatagtcc ctggattatt aatggctgcg ttggcatttg gccatctgcc ttttattttt
    16741 gtacccgcgg gcccaatgcc ctctggtatc tccaatcagg aaaaagcaag agttcgtcaa
    16801 ttgtacgcag caggccaagt cgacaaaaat gccttattgg atgtggaagc agcctcctat
    16861 catacggctg gaacatgcac attttatggt acggccaatt caaatcaatt agttattgaa
    16921 ctgatggggc tgcagctccc aggctcttcc ttcgtgaatc ctaatacccc gttgcgcaat
    16981 gaattaacca aagcagcagc atgtcagatt ttgtcattaa ccgatttaaa ttctgattat
    17041 atgccaatag ggcagatgct tgatgtgaaa aatattgtaa acggtattat cggattattg
    17101 gcttcagggg gatcgaccaa tcataccatg cacctcgttg cgatagcggc tatggcaggg
    17161 tattgggtaa attgggatga ttttgcgcaa ttgtcggcaa ttattccatt aatcgctcga
    17221 atttatccta atggtcaagc agacatcaat cattttcaaa gagctggagg catggcttat
    17281 tttgttaaaa ccttgctaga ggccgagtta cttcatcctg atgtcaacac agtcgtgggt
    17341 tttggactgg ataaatatac gcagcagccg caattgattg acaatcgagt ggtttgggtt
    17401 aatggtcctg acagctcaag caataatgac gtattaacct ctgctgccga tccatttaaa
    17461 cctcagggtg gtttgcaggt tttaagtggc aatcttggta gagcaattat taagacgtcc
    17521 agtctttatg atgagcatta tgtgatagaa gctcccgcag tcgtttgctc aagccaagag
    17581 gaatttgaac atctattcaa tcaaggtttg cttaataaaa attgtgtggt ggtagttcgt
    17641 tttcaaggcc caaaagcatg tggaatgcca gaattacatc aattaacgcc aaaacttggt
    17701 gtgttgatgg ataaagggtt tcatgtggca ttggttactg atggtcgaat gtctggcgca
    17761 tcgggcaagg ttcccgctgc aattcacgtt actccggaag cggttgatgg gggcgggatt
    17821 gctaaaattg aaacgggcga tatgattttg atcgattgtg aaagaggaat acttcaatta
    17881 ttagtttctg atgaagagtt agcaggccgc tctccagttg ctatcgaagg gtattcctct
    17941 gttctaggca tgggacgaga gttgtttggt aatttaagat tacattttac tggtgcagaa
    18001 cagggggcat gtagtctatt taaaggaaat gaattttttt atgatgcctc atgatttagc
    18061 gcagtatgcg attgttgcgg acataggcgg tacttttgct cgtttcagta gagttaatct
    18121 tgtcaatttg cagatggata aaattgaaat ttctccttgt gccgagttta ttagtcttga
    18181 gtccgcgcta ttaacttata aaaaccggca ttctttgcaa gaaataaaac atattgcgat
    18241 agcgattgcg tgtccagtca tcgatgattt ggtttctatg acgaattgtc attggcaatt
    18301 ttcaataaca gagttaaaac agagacttgg ccttgaagtt ctggaagtca tgaatgattt
    18361 tactgcgatt gcgatgagtt tacctgtatt atcgactcag gatctggtgc agattggtaa
    18421 tggttacctg gatgccagta aagtgagagt ggtgttaggc gcaggaaccg gtttgggtgt
    18481 tgcttattta attcctcatc aacatcacta tagtgctttt gcaggggaag gggggcatgc
    18541 tgattgggga gcaaaaactg agcaggaatg gtttatctat cgttatttaa aaagtaaata
    18601 ctcccatgta tcttatgagc gtttgctgtc tggccaagga ctggaaaatt tatatcaagc
    18661 tctagccgcg tatcatagta aaaaagttga atttttgtca gcagcgcaaa ttatatcttt
    18721 ggcattaaat caggaatgtt ttatagcgca taaggcagta gcccaatttt tttcaagttt
    18781 gggatcgttt gctggtgatt tagcattaac ttatggagct tttggtggtg tttatattgc
    18841 aggagggatt atgccgcgtt tactttcctt ggtacaccaa agcgacttta gaatccaatt
    18901 tgaagacaaa ggacgattta gtgattttaa tgccttaatt cccacttatg tcattgcagc
    18961 cgctcagcca ggtattttag gcgcgagcgt cagtttaaaa caaatattga caggagcttc
    19021 tcatgtcctt ttctgaatgg aaaacacaac caggcgctgt atttgctgca tctccggtta
    19081 ttccagtcat tgtaatcaaa gagttagagg atgcgttacc tttggtcgag gctttatttg
    19141 ccggaggaat tcatgttctt gaagtgacat taagaacacc agtggcaata aaagcactgg
    19201 aattattgaa caatacgttt ccagatgtat taatcggtgc cggaactgtg attaccccgg
    19261 gtcaacttca tgatgtggca gcagcaggcg ctcgatttgc catcagccct ggccaaacaa
    19321 gggaattatt aatagcagga cagaaaagtg aaattcctct cattccaggt gtagccagtg
    19381 tttctgaatt aatggaaggc ttggggatgg gatataacca ttttaaattt tttcctgcgg
    19441 cagcagctgg aggtatccct atgctgcaag cgatatcggg ggtattccct caagttaaat
    19501 tttgtccaac aggtggaatt aattccaaaa attatgagga gtatctccgc ttgccaaatg
    19561 tagcttgcgt tggagggtcc tggattgtac cggaagaggc tatcaagaat cataactggt
    19621 ctttaattac agagttatgt atggcggtga gttctttgaa gcgagaactc tgctcgcaga
    19681 attaatctat tcgagttgaa aaagcgtttt cacgtgatgt aagggatagt accagctaaa
    19741 acagttcatt gtgtagagta ggggcagagg aaaagaattt aaaatcaagg agtgaaaaat
    19801 ggcttggatt gtcgcaataa ttgggtcaat tgccggtttt ttatttggtt acgatgaggg
    19861 aataatagca ggttctcttg gactggtaaa aaatcatttt aatctaaatg caacccacat
    19921 tggtgttatg gcttctgctt taccttttgg ggcattgttt ggtgcgctgt tgattggtgc
    19981 attcatggca tcaaagtgcg taaaacgttt cggcagacgt tctttattat cctttgccgg
    20041 ttttttgttc tttgttggag ccttgggggc cggttttgct gaaaccattt cagtgttgat
    20101 tttatctcgg ttaattttag gtttggccat tggaatggct tcagttttaa ctccattata
    20161 cctggctgaa acagcagctg tgcaatcacg tggcgcagtg gttgcaattt atcagcttgc
    20221 attgactgtg gggattgttt gttcttattc ggtcaattat ttattgatag agcagcaagc
    20281 gtggcgtgca atgtttgctt caagtgctat ccctgctttg ttattgaccc tggggatttt
    20341 gtttatgcca gagtctccca ggtggttatg cagcgttgga cgtcatgatg cagcagcaaa
    20401 ctccttaaga aaattgagag gcaaacaatc agttgagcaa gagttaaaag atattgaagc
    20461 cacactggcg aacgaaccaa aacaaggcaa ctggctactt ctattccaaa agcccttgtt
    20521 gcctgtgttg atgttaggaa caatattatt ttgtttacaa caattaagcg gtatcaatgt
    20581 agtaatttat tttgcaccag aaatattcaa gaatttaggc ttgggcagca cgactggaca
    20641 aatattagca acaatgggga ttgggttagt taatttactg gttactatta ttgccatttt
    20701 gtatgtcgat aagctagggc gacgcaaatt actgttactg ggttttgcag gaaccagttt
    20761 gagtttgttt gcattatcgt tgttttcatt aaatcatatt gcctggctat cttatttatc
    20821 cgttatttgc ttaatggttt atattttctc ttttgcaatc agcgtgggcc ctatcccgca
    20881 cattgctatg gcagaaattt ttcctcttca tgtccgtggt gccggtatgg ggatgtcatc
    20941 catgagtaat tggtcgttta atacgatagt tatttttagt tttcctgttc tgcatcaaat
    21001 gtttggtata gatgtgactt tcgttctcta tgcggtaatc tgtttcctgg ggttcattta
    21061 tgcttatatt tatatgcctg aaaccaggaa tattagttta gagcaaatag aaacttatat
    21121 aatgagtggc aagccattac ggtttttagg tagagaggat gaagaggtta atgctgtgtc
    21181 aactaaatcg gaatcttctt tgctggcttc aacaaattga tacaaatttt cctctgatgc
    21241 ccgtgttatc tttgactgac acgggccaca aattctattc tttatcgcat tacctgatcg
    21301 tataaaatcc taataggtat tccagcccat agtttttatt ctattttcca attgctctct
    21361 taaatgaata gctcgcaaga cggaaacgta actccaggtc aaggatgtcg ctccttgctg
    21421 aactccggta ttcaaattaa tttgttcact taaattcaga tcagggccat attgttttat
    21481 cagccttagg taattgtctc ctttctttag atagttctgt atatgaagtt tattactgct
    21541 gttcggggac agattatgag ccatggtaaa ataatattcg gccatcgtag ctgttaagat
    21601 aaaccagggg ttccctatgc tgtttgtttg gtagccatca taagtatcgc cgggataacg
    21661 accaaacaat attgctccag aacggttttt attaatagga aacatcaaat taaattgctc
    21721 atgcaaagcc tttgcagtat ttcgcacaaa ggtgtgatgt ggcgcaaaaa caccttcttt
    21781 ttgtgggtta gttaaaatac ccaacatgac ggacgaatca agctctagtg ttttttgagg
    21841 accagggtga ggtaataatg tggcttgaat tattttattc tgatggtcca aatgctgttg
    21901 cagtcgactg ttaattaggt tggcttgcat ttcataaaaa acagcagctt gtctgtcatg
    21961 aagttggtga gccaaaatgg cgccatcggt cagcgctttt tgttgtgcca tagccgtgaa
    22021 aaaatgatgg ccataaacct cttcccacag gtcaaaattt gcatcttgcc aatgatgtgc
    22081 gatgtattcc aaatccatct tgatagtgcc catggattgt gggtccatgg tgttgttata
    22141 aagatgtgat ttgacatagc ctatttcatt atgatcgaga agctgttgcg caaaacgaat
    22201 taaaacagat gctctgagtg ccggaccatc attttgtggt cgtccccatt ctccatcaaa
    22261 aggattgcca ttgatataaa atttgggttc acccaatata tcctggccag caatgggatc
    22321 agcttgatgc tgtatttttt ctgtccatga aacgtattct aataaaagtt ttttatatcg
    22381 agcagattgg ctggcttcat accatgtctc aaccaatccc atggcaatcg ctgagtctcg
    22441 aatccagtca taataataat tggggttgta ttgcgagggt gaggcgacga tggctccatt
    22501 ggcttgaaag ttatttaaaa aatgtttttt taatatttga acctcttcat gggtaaatac
    22561 tgaagccata gtttgagaga caaaaaaaat cattaagaaa aagatccttt tcaacataat
    22621 ctcctgatag tagttagttt taaaaaagaa ggtcgccata gtacaggaaa tgagtttttc
    22681 agaccatagt ctttagggcg gattattgta aaatgtgttt aacatcaatt actttttgcc
    22741 ttgaccctgg ttttttgcaa gtccttactg aaagctttct cagattaagc tctgtctttg
    22801 ataaggtgat tgtcttcaat ggattgatac tcaagaatat ctccgggttg acaatgcaag
    22861 taagcacaaa tagcctcaag ggttgcaaaa cgtattgctt tggctttacc attttttaaa
    22921 atagataagt tagcttcagt aatgcctata gcttcagcta aatctctaag tcgacacttt
    22981 ctttttgcca tcatcacgtc caaattaaca ataattgaca tcgattgaaa ctccttaaac
    23041 aagcgggata taaactgatt gagactgcgt aagaaaatct cttgatgtcc ttacgctgaa
    23101 gttcacatta aatggttaac aaggcatccg tttttaattt atgtgcttct ttaataatcc
    23161 aagaggcaat caaaatgata aaagcggtaa ttaacgtgga caggttcgct gttcccaagg
    23221 tgatgcttgc gatacgttga ccagatggat tggtaaaagt cagagctgcg gttatcaaag
    23281 gttgataaag caattgtata agttcacctg agatcaggta aatactgata ttcttaatca
    23341 gtcgaatgtt ttcttcttca aataaatagc catgttcata caggcgaaac agctttgcca
    23401 attgatgaca aattagaaca ttaatggata agggtaatgc ttcaatcaat aaaataatga
    23461 aacgatgcaa caaggaaaaa tgagaagggt ctttcactgg agttgataac agggagtgcc
    23521 atcctcccca attcagcatg ctctcgaaat gaaatagaat gaaataagtc gttacaaggg
    23581 gaaggccata ggctaatgtt ttgaataata gaaaaagcaa atgactcaca gattttattt
    23641 ttttcattat ataattgtcc ttctaagatg agatcatgcg tattttatta aatatttatt
    23701 gttttgcaat aaatatttaa tgcgaaacat aattttatta tgactgccga tggtgaaatc
    23761 aataagacgg cgggttaaaa ttgctcgtta acaatacttt gaattgcttt gatccactca
    23821 ggatgatcgt tcatgcaagg agttaatata aattgctcac cgccaagttt ttcccactgt
    23881 tcttttgcgc gaattcctat ctcctcaaga gtttccaggc aatctgcgac aaaagatggg
    23941 caggagacca caatgttttt aattcctttg cttatgagtt cggcaaaaat ttcatcggta
    24001 taaggtttta tccatggagt tttcccgagt ctggattgga aggcggttgt gtattggtgg
    24061 gtgcctaatt gcaattcttt tgccagtaat agactggtct gatagcattg agccctataa
    24121 catgcttgat ttttatcgta tatgggtgtg catgtttggt ggcaaagcgt atcgcacccg
    24181 cttttgtgaa tatgccgttc gggaattccg tggtagctaa aaagaacgtg gaaattgtct
    24241 ttaatataag gcttcataat tttagcttgc gcctggatgt attctggtct ttggtagaaa
    24301 tctcgaatga ttttaattga tggaataatt tcttggtttg ccaaatagga tagggttttt
    24361 tcaatggctg aacctgtagc ggcagaagaa tattgaggaa ataagggtaa aatggtaagg
    24421 gaatggcaat cttttaattc tgcaagtgct gttgtaattg atggagttcc atagcgcatt
    24481 cccaaagcaa ttttgtactc atccttaagt gcggactgta attttgtcac tagattttgg
    24541 ctatgataca aaagcggaga tcctttttca gtccatattg attggtaagc ctgagctgat
    24601 tttggcgatc taaagggtaa gatcaaacaa tataccagga tatagcgcgg gatagttggt
    24661 aagtctatta ctcttttgtc agtaagaaat tcacgcaaat ataactttac cgccctgata
    24721 tccgcattat cgggagtgcc aagatttaac agtaataaac cacgtcgcat cagctaaagt
    24781 ccagttataa aatgaaggac ataatacctc aagttgatgg acgcagcagc ataaatatta
    24841 gttatactgc cgcgcttaat agtttatcac attaattggg ttaagctatt tcttcttgtt
    24901 cggcgttttc actaagagca acgacattag aggcatgtaa gcctctttca ccattaacga
    24961 catcataggt gaccgcctgt cctggaacca gcgttttgta tcccgtgccg tgaattgacg
    25021 agaagtgaac aaaaatatct tcaccgccac cttccgggat aataaaaccc caacccttgg
    25081 cgttattaaa ccacttgact tcgcctctag ccatgaatcc tccttatgct aaagcttatc
    25141 aatcgtcgac tgacataagc ggtcagctga gcaattgcat tcttccttaa atgacaattg
    25201 ttacaagcct attttacaat aggtcatttc aacataacgc aaaaagggat cattactcaa
    25261 taggtttatt aaaatttatc tctaattctt atgatctcgg agcaatgttt ttgttacaat
    25321 gattgagtac ttattgatag agagagtatt ttgttttgca ttgataatca gattaagtcg
    25381 tcttctttta tacttggaga gtggccttta tctacagttc tgttaaaaaa tgacgcctca
    25441 tacccctggt ttattttggt tccaagaaaa atggatattc aagaaattta tcagcttgaa
    25501 aagagcgatc gatttttgtt aattgaggaa atcaatcaac tgtctcttct ggtgagagat
    25561 tactttaaac cagacaagct taatataggc gctcttggca atattgtttc gcaattgcat
    25621 atacatattg ttgttcgcag aaaaaatgat cctttgtggc ctcaaggtat ttggcaatct
    25681 tccatgcttt ctattcctta ccctgaaagt gaattaaaga gtttactgcc taccttgggt
    25741 gatttagtaa acaaggcata ctaaggaaca ttgttatttt gtatttttgg aaatattgat
    25801 gtcgctcata acaccaatac gtgtgaggct ctctttaggg attttcacac gtattggcag
    25861 caacaatcat gttaattgat gcaaagtgaa cgcatttccc tctgaatcca cagcaatcgc
    25921 cattctgcaa acaggtgaag cgaataggtc aattttgaac tgaactcctt ttgattttag
    25981 ttggaatacc agatcgtcaa gattatctac ttcaaaagca acgctacctc cggaattagc
    26041 gctggggata actccttgag ccaatgtagt aattgcaaaa caaccccctt gaggtaaatc
    26101 atattctacc catgctccct cagcggaaat tttaccgact ttaagtccaa gtgttttttc
    26161 ataaaattgc cttgctctat ccatattttg aactggatac atggtaaatg ctattttttt
    26221 gatcatgaga gctccttgtt gaaatttaaa tatactttca ccattagatg aattataaat
    26281 tacaatgata aacattcact ttgaaataaa cgataatgtg catgcttaaa aaattaatgt
    26341 tcttattatt gttgggagca ggtttgttaa gctgtggtca aagcactgat cagcaaattt
    26401 ccaatattat aacgccgaag caattcccct ccagtcccag ggattatcga ccagttcagg
    26461 aattgcctta tttggcctgg tggaagcaat ttcaggatcc agaattggat cgtttaattg
    26521 atactggatt gaagaccaat atggatattc atattgcaat ggctaattta cagcaggctc
    26581 aaggagagtt acagcaagtg aagttgagtt ggatacctaa tgttcaaata ttgggagggt
    26641 attcaactaa tcctgctctt ggtgtgccag gagggtttta tggaatatgg ccaagttata
    26701 ttttgaatat tatgcaactt tatactcagc aaaagcgtgc tcaatatcag gtccaatatc
    26761 atcaggcagc aatcgatggt ttgcgtttga ccttgattgg tcaggtggca gcggcttatt
    26821 tcactttgat tgcacagttg gagcaactta aattattgta tgaactggat agggatttaa
    26881 agtcgttaat tgctttgagc aggaaagaaa tcaacatagg attgaaaaat gatattgatt
    26941 tggctcagtt gcgatccaac gagcgcttga ttgcagcaca aataaaaccc attaaacaca
    27001 acattgtcgt tagtcagaat gcgttgcgtt atttaataaa tgagaatcct ggtttaatca
    27061 aaagcaaaaa taattttgct caattggatt ttacccgctt taaacccgga agtttacccg
    27121 ctactgtatt aggtaatcgc cctgatatga agatggcgga gtattcccta aaagcagctc
    27181 gtgcaggtat ttttgttgct tatagtgaat tttttcctgt tatgcagctg gatgatttta
    27241 ttggagaggc acatttgcct gatagcacgt ttgaacaagc gaccgatgcg tacgtaaact
    27301 ggacaattaa tccaaatact ctgggtaaaa tttcagttag caagggagtt tataatgcaa
    27361 aattggccga gtacgttaag acagtccgat taattttaaa agaagttgat aatgcttttt
    27421 cagctaataa acgcatgaga gagcaatttt tatcttatcg gcacgcacaa gaagattatc
    27481 agcataaata caaattacaa aatggtttat taaaatcagg gttaatgtcc tataaagagt
    27541 tattggagag taaggtttat ctggataccc tggcgctctc aacaaatcag gctaagctgg
    27601 aattggccat gtcactggtt atgttgtatc aagatttggc tggtgggtat gcctgtagtg
    27661 actaaatcag tttgatttgt aacacattga ttttaaaagt cgccggcggg ctaaaattgc
    27721 agttctcgtc tcttgagctg ttcgatgaga taagttaatt tcgcattatt gttatatacc
    27781 aagggaaact ggttggctat tttatgaaaa aattgatgga atatggtgga aaaataaaag
    27841 agtacttgct tcgtttaaaa ccaaaacgct ggtttactct tactaatgtc attattttta
    27901 tcggttttct tactgcaatt atctatgttt tttcctattt gatccctttt actgataatg
    27961 cgtttgtagt aaacaatgta cgtccggttg cggcattaac aaacggatat ataaccgagc
    28021 tttatgtaaa aaatggtgat gcagtgcata agggacaaaa actatttacc gtattcaaaa
    28081 agccctatga atacacagtt gaacagctca gtgctgattt agctggagca caggcaaagt
    28141 tggcagtatt aaaagctacg tacgaacggg atttgaagct tagcgagaac gagcaaaaaa
    28201 tatacaaaaa gttggcgcaa gatgatcaaa aataccttaa agggtatgct ataaaatctg
    28261 tttctctgat taccctgcaa aactcccagc aagaaacaaa agcagctaaa gataaatggc
    28321 aggcttcatt aaaacaactt gaaatagatc agcatcaaat aacagttcag gagaatgaaa
    28381 taaaatccat tcaggctcgg ttaaaaaatg ccaaagtcaa tttggattta acggatgttt
    28441 atgctcaggg taatggagtt attcaaaatt tgttttttac cataggaact ccagtcaata
    28501 tcaatcagcc attattctca ttggttgatc aggataatat ttatattcag gccaatttta
    28561 acgagacgga tttgcgtgat gtgcgcaagg gctcaaaggt attgatattt ccaagaatgt
    28621 atttaggaag aaaaatattt catggtgtta ttgattcgga ttattggtct gcgaataggc
    28681 aattggtgga tgatcgtact cagctgcaaa atgttatcaa tgaaaatcaa tggattttat
    28741 tgccccagcg ccttcctgtg attatcaagg ttactgaccc tgaccctaaa tacccgcttc
    28801 gtgttggagc tagcgcttat gtctatgtcg aagtttattg aatggattga tgaggttgat
    28861 ccttatgctg ttcaacgaat agcgctttat aaatcacttt tcattgccac agtaatggcc
    28921 tatgtttatt gggtatttcg tcccacgaat tttacggcat ttttttcacc atttttgctc
    28981 gtaagatttt atgaatcccc cagtcttacc agctttaaag agaaagaaca ctttttgatt
    29041 tttattggag tggtagtggt attgttgagt acctcatttt atcttgttta cccatttcgg
    29101 gttgtatttt tctttttctc aattattgcg ttagcaagcg tttattttta tgtgctaaaa
    29161 aattatcttc cattgaggaa tgtgactatg ctcattattg cttctggagc gacaatttta
    29221 agtacagaac ctcctgcaaa ttggcaaatt gtctacgaca tcgttgggtc aagcgcttta
    29281 tctatgatcg ccatattcat ttgtttaaga ttcttcccca atcaatatct cagcgtatgg
    29341 aaaagggctt tggcaaaatt tattcaatcc cttgagctgg atattgaagg ttcttttact
    29401 caccgtggtc ccaaattcat gcaggaagaa atgactcatt tgggtatgct taggcaatat
    29461 cgtagactta tcccccaaaa atacatgatt tatactcagc gaatttcaat caacatacgc
    29521 aacattcaat tttctttgga taatctttat tatgaggcca aaaatgaggc tttctggcat
    29581 ggagtgaaag agaatcttta tactcttaga tgtgcgatta aaaccaacac atcctgtggc
    29641 actcctaaaa tgtccatcat gcctgagtca aaattacaac aatatgttat gagatgtttg
    29701 caacaagcat ttagccaatg gaatcaatta tgcatgatac ttcagcattg aagaaaaaac
    29761 ttgattatgc tcgatttatt catctagtca tcatgttcat ggttgccatg ctcatttttt
    29821 tgttttcgac ttttcccgaa aagtggtggg tattgataac tgttttatca gtaagtgcag
    29881 gtattgaacc aggattgatt atcagaagag ctaaacatag gataggaggg acactgttgg
    29941 ctctcatcat tttgattccc ctactttatt tattgcaatt gaattatcga ttgatatcaa
    30001 tcttatttgt tctggcgata gttggtttga gtgttgccac cttaaacaca agacgttacg
    30061 acattagtgt attttttatt actttagtag tctttttttt gatggcgcaa acggaagagg
    30121 cgacatcgcc tcgtggtcct tttgagatgg ttcttaatcg aggcatatgt acactgtttg
    30181 gtgtttttat tgtgttggca ggcgattatt ttttatttca ggcataccgt tattctcata
    30241 gactttattt ttttcatcag
//
LOCUS       DASLOE010000021        30012 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.021, whole genome shotgun sequence.
ACCESSION   DASLOE010000021 DASLOE010000000
VERSION     DASLOE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.021"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(50..1840)
                     /gene="oadA"
                     /locus_tag="U6309_000761"
     CDS             complement(50..1840)
                     /gene="oadA"
                     /locus_tag="U6309_000761"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HEN5504215.1"
                     /translation="MSKTFITDVTLRDAHQCLIATRMRTEDMLPICNKMDDVGFWAME
                     VWGGATFDACLRFLKEDPWSRLRQLRQVLPNTQLSMLLRGQNLLGYRNYADDVVRAFV
                     KLAVNNGVDVFRVFDALNDARNLKVAIDAIKSHKKHAQGAICYTTSPVHTLDNFLELG
                     KKLAEMGCDSIAIKDMAGLLTPTVTVELYAGLKQATGLPVHLHSHSTSGLASICHYEA
                     VLAGCNNIDTAISSFSGGSSHPPTEALVAALTDTPYDTELDLNILLEIDDYFKAVRKK
                     YSQFESEAQNIDPRVQLYQVPGGMISNLYNQLKEQNALDKMDAVHKEIPRVRKDLGYP
                     PLVTPTSQVVGTQAVINVLTGERYKTITNEVKLYCQGKYGTPPGKISSALRKKAIGRT
                     EVIEVRPGDLLPNELDQLQNEISDLALSDEDVLLYAMFPEIGRQFLEQRKNNQLIPEP
                     LLTQSSAPDNSVMSEFDIILHGESYHVKVAGYGMMEHGQQSCFLWVDGVPEEVVVQHS
                     ELHDKIERSYVNNKIGPGDITVAIPGSIIAIHVSAGEEVKAGQAVLVIEAMKMETEIK
                     APANGVVAEILCQKGDKVTPGQVLIRVEVS"
     gene            2042..2479
                     /gene="aroQ"
                     /locus_tag="U6309_000762"
     CDS             2042..2479
                     /gene="aroQ"
                     /locus_tag="U6309_000762"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEN5504216.1"
                     /translation="MKKILVLHGPNLNLLGSREPSIYGHASLTQINGDLIQEADNAGI
                     RLSCFQSNAEAELIQAVHQAGIDKINYIIINPAAFTHTSIALRDALSAVAIPFIEVHL
                     SNIFSRETFRHHSYFSDIAVGVISGLGTKGYLLALQAIIKELK"
     gene            2493..2975
                     /locus_tag="U6309_000763"
     CDS             2493..2975
                     /locus_tag="U6309_000763"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEN5504217.1"
                     /translation="MDIRKIRKLIELLEETGISEIEIKEGEESLRLSRHSNIVAETAP
                     QIHYVAPSAVKQTASAPSSNTSTTEPHVIENKQVTSASSGHKIRSPMVGTMYTAPSPE
                     SPPFVTVGQSVKAGDTLCIIEAMKMFNEIEADRAGKIVEILVKNGDPVEYDQALFIIE
                     "
     gene            2988..4331
                     /gene="accC"
                     /locus_tag="U6309_000764"
     CDS             2988..4331
                     /gene="accC"
                     /locus_tag="U6309_000764"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEN5504218.1"
                     /translation="MLSKIVIANRGEIALRILRACKELGIQTVAVHSDVDKDLLHVRL
                     ADETVCIGPAPAQKSYLNIPAIISAAEITDAVAIHPGYGFLSENADFADIVEQSGFRF
                     IGPRGDSIRLMGDKVSAIAAMKAAGVPCVPGSDGPLGDDDNKNLELARKIGYPIIIKA
                     AGGGGGRGMRVVHSESNLLSAIALTRSEAKAAFNNPIVYMEKFLENPRHVEFQVLGDG
                     KGHAIHLGERDCSMQRRHQKVVEEAPAPGITPELREEIGASVIKACKELQYRGAGTFE
                     FLFQDGCFYFIEMNTRIQVEHPVTEMITGIDLIKEQIKIASDIPLTLTQKDIKLRGHA
                     IECRINAEDPRNFMPSPGTIKLLHQPGGPGIRFDSHIYSSYVVPPNYDSMIGKLISYG
                     ATRAEAIARMRNALDEIIIDGIKTNIELHQRILHDKSFMEGGTNIHYLEKMLKDS"
     gene            4333..5202
                     /gene="prmA"
                     /locus_tag="U6309_000765"
     CDS             4333..5202
                     /gene="prmA"
                     /locus_tag="U6309_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEN5504219.1"
                     /translation="MWFQLKIEHCPNDKIEEITEELEEYGALSITLTDKNDNPVLEPE
                     PGTTPLWPEVIIHALFAQAEEAQYAREQLVAKRPSLHCSLELLADKNWERAWMDDFRP
                     QRFGNRLWVCPTWLPPPEPDAVNLMLDPGLAFGTGTHATTSLCLTWLEQADLKNKSII
                     DYGCGSGILSLAAIKLGAKHVYAVDIDNQALQATQSNAHANHITESQLSISSPEALQN
                     PVHLIIANILLAPLISLKERFHQLLPSGAHLVTSGILEEQAPLLIDAYDSAFTHIATE
                     YCEGWSLLVFTSK"
     gene            5229..6818
                     /gene="purH"
                     /locus_tag="U6309_000766"
     CDS             5229..6818
                     /gene="purH"
                     /locus_tag="U6309_000766"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEN5504220.1"
                     /translation="MAKKQIYSSFKPRRALLSVSDKRGIRELGQALHDHGVELIATGN
                     TAAILREHKLPVTDVSECTGFPEIMDGRVKTLHPAIHAGLLARGEQDSPVLKQHGIKP
                     IDLLVVNLYPFEQVINHPDCDFNKAIENIDIGGPAMVRAAAKNHAHTYVIVDPNDYSK
                     LIHYLQDQKAPSHWNFALAKKAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFN
                     KITDLRYGENPHQQAIFYADKNSHPGSLGTAALLQGKQLSYNNILDADAALDCVKSFS
                     NEKSVCVIVKHTNPCGIALSDTSLDAYLKAFQSDPISAYGGIIAFNGTLDSDTAKAIL
                     EKQFVEVIIAPDANEEAKKILAAKENIRVLLTGFWQQGNNFRLSMKKVDGGLLVQEHD
                     SLSLESCELQTVTQVKPTDEQLQNLMFAWLAAKHVKSNAIVYANDLATIGIGGGQTSR
                     VMSARIGLWQAEQMGFDPKGAVMASDAFIPFPDTIEIAAKAGISAIIQPGGSIRDEKI
                     ISCADQHNIAMIFTGVRHFKH"
     gene            7103..7888
                     /gene="icmH"
                     /locus_tag="U6309_000767"
     CDS             7103..7888
                     /gene="icmH"
                     /locus_tag="U6309_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HEN5504221.1"
                     /translation="MTTEQYPSSLVNRLAITEPALVPSGYYRSKLFIAPFSTNVLVAA
                     AGPILSLLERLCLSTSLPPVEDIRENIQHELRAFHSKLDASKYPLDIISIAQYLLSAT
                     IDEILGKSYLRVYNLTTEFKSFTPLSSDGAQPQQRFFEILNYIKERPNQFLDLIELAY
                     FCLIAGFEGEYHLKADGRQCLDNTIEDLYQIIQKYRFNKPHRLFNENPLPKTIKKNYK
                     ATWITTIAAVGIVILAFVTSHVLLENKAKTVLFGHTQLAMLEN"
     gene            7888..10809
                     /gene="icmF"
                     /locus_tag="U6309_000768"
     CDS             7888..10809
                     /gene="icmF"
                     /locus_tag="U6309_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmF"
                     /protein_id="HEN5504222.1"
                     /translation="MDNSLRALCDAIKKILSQLKPQANQLSFIVVTGKNAQGKSALLK
                     QSNMEEMPVFSEEHAKLYFNQKGIIVELGENWLTNSKTLLLTTLKQLNKCNRHLKITG
                     LILCVDVNDLLIAEPAQFTEQKKSHLQLIQRLGVNLGYQAELALIFTKMDTLAGFSEF
                     YQMDHVNDLSKPLGFSLDCVNQLSKKIETFSLQFNQLIEALGQQVINKMHPARSTIKR
                     SLIREFPLQLASLRAPIQSLIQGISPKLFHVHSIFFTSAEQGGVSVDRLNKKIQHEYA
                     LVVQDTFPQATNFRSYFVEGALQTIQEQCIQVPQTTKFSQKPFIAVAVSLAGLSLLTL
                     IYNHYKTSHILDEASKELLAYEALNNQGTSGAQALYHLSQAANKMNHITSNSISLPTV
                     HQLKLNLHNNAQNRLQGEFLPSLANELEQVITSPGNTPITRYKALKIYLMLGDSKYFS
                     SQPILDWFQQQWQGRAPGTTQKQLALLKQTLSNPPQNIPIKSQIISDARNYLNALPTS
                     YLYYSIAKEYFPQEKQKIVIEGFSIPTDEVPTYFTKTGFTKIINELPKITSTLQSENW
                     VLARQDLSQLHNMLIQAYCFDYVTWWQTFMKKSQPLRYQDYQQGRQLTKLIQQSNSFY
                     KFANLIQQETKPDLTEGISTFNQFIANQFTDLNLTTLSSIKELDFRISELERFIATLS
                     VVNDGGKTAFTITRSRFLNENSSDPVSLLYSQARQLPEPLSSWTKQIAGDTWVILIRD
                     SRQYINNQWQKTVYREFQNSIAQRYPFDSSQTAEIDIADFNRFFSTHGLLNTYSEQYI
                     KPFLDTSSPEWKPKAVNDFILPISNEVIDELIRANIITNMFFPDHSDESKIDFSLQKI
                     SLDPVVSNLVLEIGGTKLMDNQTSDSLIRFTWPQNNAKLALDSIEGNHYELAEYGTWA
                     IFKLLEKVNVLVDEQDSSSLQILFEVNSNSGRYLLKTNNQINPFTPGILNGFTLNDHI
                     A"
     gene            10812..12068
                     /locus_tag="U6309_000769"
     CDS             10812..12068
                     /locus_tag="U6309_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5504223.1"
                     /translation="MKQNTKHSIIAGLYGNALEWYDFLLYASFAPVFAEIFFPSKIHF
                     VSLIATFSLFAIGFIMRPIGGLLLGHYADHVGRRKALILSMSVMTISTALIAVLPDFN
                     SWGIIAPLLFSILRLIQGLAVGGELPGSTTYLIEHMFQQSRGLAGSLVLSSAFLGIFI
                     GALTASIFSTLFTGDALLNWGWRYAYLLGGILGILGIYLRIKSFESPTFLKDKHTAEL
                     PAKVVFTRFKQQLFLSVIITSILAMSNYVLIAYVTTFLVKSEYFLLKDALLINLIALA
                     LLTVLIPLMGFLSDVVGRKPIFLIGLFSLCILIFPFFWLLLSGSWWKALCSELLLAIA
                     LAPINATVPTIIAEMFPTSVRASGISISYNIGQALFGGTMPLVAFTLIELSSNKMAPA
                     WYLFLWILIVIPITISIRESYQKELT"
     gene            complement(12214..15243)
                     /gene="icmB"
                     /locus_tag="U6309_000770"
                     /gene_synonym="dotO"
     CDS             complement(12214..15243)
                     /gene="icmB"
                     /locus_tag="U6309_000770"
                     /gene_synonym="dotO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmB/DotO"
                     /protein_id="HEN5504224.1"
                     /translation="MANWSESFFEGVDTFFAWLSTSLKQTTESYIDLETADSPTVLVN
                     HDGSLLSILKIEGITGLAGPEEFERLVEGLANSFQAAMGRPGHALQVYFSHDKQNIVK
                     MIKDIYDPAEATASRLELNLRDLFEERVNYLSQYCAEERVYFVLYTRPFNLASDQLKA
                     ANKAKMKMIKDTKAPPFKNTQTIFAAVPEIRDTHDAYVRAILNDLDALNVIAKLLEVH
                     DAVHAIRMTGDPDYTADDWRATLPGDKIPVRELNNFEGDPSDLLWPPLSKQVLPRDAE
                     IIDLRTVRVGDKIYASTYIDLFPKDVRPFISLFSRILPSHVPWKISFLLESEGLSTIK
                     LKGLLAAILSFSSAQNRLISDSVNLLKYIQLNTDEAIVRLRVVATTWAPEGNIPLLRR
                     RSSELVKAIEGWGSTDVSEICGDPFAGFVSSMLATTLNSSAVASVAPLSDVISMLPIT
                     RPASPWKTGALLFRTPDGKPWPFQPGSTEQTTWIDLVYARPGSGKSVLSNALNLALCL
                     SGGLMRLPRIAIIDIGPSSSGLISLLKEALPASKRHLVAYHRLRMTPDYSINPFDTQL
                     GCRYPTALERSFLVNFMTLLTTPLGAAKPYDGMADLAGMVVDELYKSMADEFNPTPYA
                     PGIEEFIDSILEEIGFVRDAKSTWWEVTDSLYSAGFVHEAMLAQRYAMPLLADAASIC
                     RTPSIEDLYEKITAPTGESLINAFSRMISSAVREYPILSRVTSFDIGDARVVSLDLDE
                     VAKSGGDAADRQTAVMYMLARYVLARHYYLTEESLNNVPEQYKEYHKQRVLEIREDHK
                     RIVYDEFHRTAKSSAVREQVIIDMREGRKWKVQISLLSQAVDDFDPVMIDFATAIYVM
                     DAGPSQAVEKTSQIFGLSETAKIALRTRVHGPRQGGATFLAQFATKTGINVQLLTLTL
                     GPVELWAFSTTAEDATVRNLLYRHLGPSEARRLLAALFPNGTVTKELEARLASMKQKV
                     GLIEDDEREGMINQLVNDILDAYSKDPNVKSLPAKVG"
     gene            complement(15279..15905)
                     /gene="icmJ"
                     /locus_tag="U6309_000771"
     CDS             complement(15279..15905)
                     /gene="icmJ"
                     /locus_tag="U6309_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmJDotN"
                     /protein_id="HEN5504225.1"
                     /translation="MADNQQRCELKLIASPGSWRLYSARKIDERFKSYEQKIFQRDRY
                     TCQFCGFQARLYQDIVNLDGDYTNNRLSNLVTACCFCAQCFFVESVGVGGYGGGTLIY
                     LPELTQAELNSLCHVLFCAITNDTGYKSSAQNIYRSFKFRSQIVEEKFGEGTSDPAIF
                     GQLMIDSGVNSEEIREKLFKNIRLLPSRAKFRKQIEKWAASALEEIAD"
     gene            complement(16103..16501)
                     /gene="icmD"
                     /locus_tag="U6309_000772"
                     /gene_synonym="dotP"
     CDS             complement(16103..16501)
                     /gene="icmD"
                     /locus_tag="U6309_000772"
                     /gene_synonym="dotP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmD/DotP"
                     /protein_id="HEN5504226.1"
                     /translation="MNIKVIHKSGYKHWLVSAACLALLTLICQDAAAGVSLGKMASQI
                     TGSFTSLTKLITAGSYLAGLGFSIGAIMKFKQHKDNPTQIPIGTPIALIFIAAALLFL
                     PSILGVAGVTMFGGSGGKTAGPTGTIYKTN"
     gene            complement(16527..17111)
                     /gene="icmC"
                     /locus_tag="U6309_000773"
                     /gene_synonym="dotE"
     CDS             complement(16527..17111)
                     /gene="icmC"
                     /locus_tag="U6309_000773"
                     /gene_synonym="dotE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmC/DotE"
                     /protein_id="HEN5504227.1"
                     /translation="MADPTCTGGTVACWIVSQANILANIANQLEPVQRLITGAAYLIG
                     CAFIFKAIYSLKVYGEARTMMSSNTSIKEPVMYLMVGALLIYFPSLVSSVLQTTFGYS
                     NPLAYSGGVSSGSDTISALFGSGSLVGRPLVMIIRVIGLIAFVRGWVLIARSASQGQP
                     PGGTGKGLIHVFGGILAINIVGTVDMINNTLYGT"
     gene            complement(17119..17928)
                     /gene="icmG"
                     /locus_tag="U6309_000774"
     CDS             complement(17119..17928)
                     /gene="icmG"
                     /locus_tag="U6309_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmG/DotF"
                     /protein_id="HEN5504228.1"
                     /translation="MMAEHDQNNDEYKFAELDSYDMDQAGESDLDSEASFQSGKEGLT
                     KKKDIKRNALIAIGAVVFIMVMYKIIGWMFFSDKSSQVTSKPAIPPVTQVATPQPVQT
                     IPTTTPIQQVQPTTIIEDDPDLKKKVSAIEMTQQSLRSEVNALGEQINAVNNNIKNLN
                     AQIVNLNQIIGNMSNQIARQSEVINVLMARTTPKKVVKVSRPTVQARIIYYIQAVIPG
                     RAWLIGSNGSTLTVREGSKIPGYGMVKLIDSLQGRILTSSGQVIKFSQEDS"
     gene            complement(17943..21089)
                     /gene="dotG"
                     /locus_tag="U6309_000775"
     CDS             complement(17943..21089)
                     /gene="dotG"
                     /locus_tag="U6309_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein DotG/IcmE"
                     /protein_id="HEN5504229.1"
                     /translation="MASKKENLKSLFSNTRTRVIIIFTAALLIIAVVIGFFKIRSATT
                     SSIAAAEVSTVPGGIQSIPGVLDPTAQYAKLQEEQNITQAQVAEKTGGSAIPTIIRTQ
                     ALGEGVGVIGSQSGVGFAALAQEELGGPQRSLWIQELQDGGCSKSVITKVVNQGAQLT
                     DLKAACSCVQLKDSGYGLQELEQVCECKELKAAGYNARQLKEAGYSAGRLRNCGFDAC
                     ELRNAGFTAQEMKDGGFSDGELKGAGFSDAEIAKASGLPDGITADDVRKAGCGAAALA
                     KLRQAGVSASAIRKISGCTAEQLKAAGYTAKELKDAGFSAADLKRAGFSAAELKDAGF
                     TARDLLNAGFTPADLAKAGFSNAQIKAAQAELPPGISPQDVKNAGCDVEALKKEREAG
                     VSATLIRQYAGCSAQALKAAGFSDADLASAGFTPAQISAATPLSDAEIKAAGCDPDKL
                     KKLFSAGVSAKRIKELNGCSAEALKAAGYDAQSLLAAGFTPQELLAAGFTPKQLEDAG
                     LNPASIIADGRVADCSVESLKKARAAGVSALTIKQTLGCSAAALKAAGYTAKELKDAG
                     FTAAELKAAGFSAKDLKDAGFTAKELRDAGFSAQELKDVGFSAKDLKDAGFSAAELKA
                     AGFTAAQLKAAGFSAKDLKDAGFSAAELKAAGFSAKELKDAGFSASDLKNAGFSAKEL
                     KDAGFSASDLKNAGFSASELKNAGYSADELKKAGYTSAELRNAGFSPQESAVAGLQGP
                     DLQQFDSSITGIPSIPGATPRPTTSDAASSAEQLQAILQKQNEQLAEQKYQQEIQQRT
                     SDMLTAATQLVQDWKKVETQVYTEGTEETKTSGGESSVPGAGTVAGANNQPVEQGASG
                     AQNQAIIKTGDIMFAVLDTAVNSDEPGPILATIVTGKLKGSKLIGSFNLPSNADKMVI
                     TFNTMSIPGAEKTISISAYAIDPNTARTALASRTNHHYLMRYGSLFASSFLQGFGNAF
                     QSANTTITIGGTGGGNNITVANGVGRSTLENAVIGLATVGKAWSQQAQQLFNTPTTVE
                     VYSGTGLGILFTQDVTTI"
     gene            complement(21094..22176)
                     /gene="icmK"
                     /locus_tag="U6309_000776"
                     /gene_synonym="dotH"
     CDS             complement(21094..22176)
                     /gene="icmK"
                     /locus_tag="U6309_000776"
                     /gene_synonym="dotH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmK/DotH"
                     /protein_id="HEN5504230.1"
                     /translation="MKKYDQLCKYCLVIGLTLSMSCSTYAADQSDDAQQALQQLRMLQ
                     QKLSQNPSPDAQSGAGDGGDNAASDSTQQPNQSGQANAPAANQTANAGGDGQIISQDD
                     AEVIDKKAFKDMTRNLYPLNPEQVVKLKQIYETSEYAKASTAGTPPKPTATSQFVNLS
                     PGSTPPVIRLSQGFVSSLVFLDSTGAPWPIAAYDLGDPSSFNIQWDKTSNTLMIQATK
                     LYNYGNLAVRLRGLNTPVMLTLIPGQKAVDYRVDLRVQGYGPNAKSMPTEEGIPPSAN
                     DLLLHVLEGVPPPGSRRLVVSGGDARAWLSNEKMYVRTNLTILSPGWLASMTSADGTH
                     AYEMQKSPVLLVSWHGKVMQLKVEGL"
     gene            complement(22179..22817)
                     /locus_tag="U6309_000777"
     CDS             complement(22179..22817)
                     /locus_tag="U6309_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system apparatus protein
                     IcmL/DotI"
                     /protein_id="HEN5504231.1"
                     /translation="MAEDALTVVALRNKFYKDSQRKVILALLIAIVVNVVLASLVVYM
                     ITHPPAPKYFATSINGRITPLFPLDEPNQSDSAVLQWANQAAIAAFTYNFVNYRDELQ
                     ASSGFFTAEGWDQFLGALEQSNNLDAVKAKKLVVSAVATRAPIILQKGVLNGRYSWRV
                     QMPILVTYQSASEFTQQNNVVTMLITRVSTLNSPRGIGISQFVVGPASGGVS"
     gene            complement(22833..23117)
                     /gene="icmM"
                     /locus_tag="U6309_000778"
     CDS             complement(22833..23117)
                     /gene="icmM"
                     /locus_tag="U6309_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmM/DotJ"
                     /protein_id="HEN5504232.1"
                     /translation="MSRETWALIKRNKNFNVHIYRRGLTLLILSLGLSCGLSGLIYYV
                     HMHEPERDFYATSGITPPIQLKPMSTPNMSSEALLPPDPPDEDSQRIIPQ"
     gene            complement(23129..23698)
                     /gene="icmN"
                     /locus_tag="U6309_000779"
     CDS             complement(23129..23698)
                     /gene="icmN"
                     /locus_tag="U6309_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmN/DotK"
                     /protein_id="HEN5504233.1"
                     /translation="MRSLRTNYIYVLFKTTGLLFLLLLSACNRSGYIPENEVPKLPCR
                     VDGACDATIIKMMTDLNKKGIKVASVGQNYLISIPASALFADQSPRLNWASYSLLNEI
                     AAFLKQFRKIAITVTSYSSKYVSVKRERALTLARSRVVSEYLWSQGVDSRIIFTQGLG
                     SDKPITSYTLGGDRSPNARVEITFRRAVA"
     gene            complement(24102..26453)
                     /gene="icmO"
                     /locus_tag="U6309_000780"
                     /gene_synonym="dotL"
     CDS             complement(24102..26453)
                     /gene="icmO"
                     /locus_tag="U6309_000780"
                     /gene_synonym="dotL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmO/DotL"
                     /protein_id="HEN5504234.1"
                     /translation="MMRGIDSRHELDPTLLLRDTRTFTQRLADFFADPTNISIVLISL
                     AAVSYYFSEAATFLLIMGSIFFLYSYTRKQKLPFRLPQISRAKDYNDLKPGINKPNIA
                     RGITFFGNDRKTGEELWFANDDMRTHALIFGSTGSGKTETLVSLSYNALVQGSGFIYV
                     DGKGDNSLYAKVFSMVRSMGREDDLLLINFMTGARDIVGPQEKRLSNTLNPFCQGSSS
                     MLTQLVVSLMGSSGQSSDGDMWKGRAIAFVEALMRLLVYMRDEGAILLDANTIRNYFD
                     LQRLESIVIDKVFPRDDQESVNIETIPKLVTDPLRNYLNTLPGYNKEKKGKQVSQVLE
                     QHGFITMQLVRSFSSLADTYGHIIRTNLAEVDFKDVVLNRRILVVLLPALEKSPDELS
                     NLGKIIVSSLKAMMAAGLGEEVEGDYRDVILRKPTNAPTPYMCILDEYGYYAVQGFAV
                     VPAQARSLGFSAIFAGQDLPAFQKASKEEAASIGANTNIKICMKLEDPTETWDFFTKT
                     AGEAYVTKVDSFQTKETSIANSYMDTKSSSFEKRARVDLLDLKEQTEGEAHIFFKSKI
                     VRARMFYANPKPVKQLKINQFLKVEPPPDDYLMKLQKQLASFQSILESGDLSINKAVE
                     NEEITLISKALKESTIIEPIERGVAALIAFHGQNEPEPVEDIVEEEVEGALTIFSKLR
                     IDPNAPPILVADKEVFSEPLLPINETRNQMITIERLAGAKDKYAGTVANELIKDFQIA
                     TSYPPEERDVIDVQELTGIIRDLSAKISAEREKANKKAAEELT"
     gene            complement(26450..27580)
                     /gene="icmP"
                     /locus_tag="U6309_000781"
     CDS             complement(26450..27580)
                     /gene="icmP"
                     /locus_tag="U6309_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system coupling complex
                     protein DotM/IcmP"
                     /protein_id="HEN5504235.1"
                     /translation="MAQQQQQSGSDNSMAPVWIVILLFITAYFVWALAHQYIVSFVFT
                     INIWQARLVNLFLNNQLLANQIYLMQTLDPNTVNWDQMVTVMRAVGDYMRYPVICILV
                     VLAFVLYNSNVTLKYRKTYDMKSLRAQEQFNWPAIMPIVKEDLVSQDVNKGPWAMALT
                     PMEFARKYNLLRKDDALLDNPVPGQEMTAGIRRGDAKRVFTMQLGPYWDGFERCSPQA
                     YALSAVFMARMNRDRDAANNILKVLDKTFVDGKPDFSVARPVMKKYQNSELVQEVVAK
                     HAYVLTVIASLLEAAREDGVVPSSEFLWLKPVDRRLWYMLNCVGRQTPYSEVAGPFAH
                     WKAEKEMGRRSLVPMIDEAIRALEIAVKEVRLTPRQMEELEP"
     gene            complement(27767..28342)
                     /gene="icmQ"
                     /locus_tag="U6309_000782"
     CDS             complement(27767..28342)
                     /gene="icmQ"
                     /locus_tag="U6309_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm secretion system protein IcmQ"
                     /protein_id="HEN5504236.1"
                     /translation="MKDQLSDEQKETILKALNDAIEKGPWDKSNFLRVIGKKLIAIRD
                     RFLKRIGAASQARLKAESHLANRIALRSGQQEIYVSLYSSDGSNLQSWEKIVGSLPRQ
                     MISRPIYADEEDIKAILKTKENKQNEAYVAIYISQSDILHLSADKAPVDKLGKPLLTL
                     KDKSISLENISRFVHVSGVYRYSNGRLIKNA"
     gene            complement(28433..28795)
                     /gene="icmR"
                     /locus_tag="U6309_000783"
     CDS             complement(28433..28795)
                     /gene="icmR"
                     /locus_tag="U6309_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmR"
                     /protein_id="HEN5504237.1"
                     /translation="MGNNTDDSARNPFGFYTPPRVKEIGESDVTDATLGSVYSEIISP
                     VKDCILTVAKAVSFNPGGKDNTDAVEVLTELNTKVERAALNQPILTTKTERMFGAAES
                     EKSSEQPSHEERGFKLSS"
     gene            complement(28895..29239)
                     /gene="icmS"
                     /locus_tag="U6309_000784"
     CDS             complement(28895..29239)
                     /gene="icmS"
                     /locus_tag="U6309_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmS"
                     /protein_id="HEN5504238.1"
                     /translation="MERDISKCMAKIAASMNAKFYLNDRFVSFDEVFSETGLLPAIAK
                     RADQLCSLCLGYGLGATYDEAEGALLGIRVVFDEVTPNVLRLLCMTDVMNELIQGGPS
                     RDYTPLDELMYD"
     gene            complement(29240..29500)
                     /gene="icmT"
                     /locus_tag="U6309_000785"
     CDS             complement(29240..29500)
                     /gene="icmT"
                     /locus_tag="U6309_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmT"
                     /protein_id="HEN5504239.1"
                     /translation="MAGGFSATSHWRDSARSARFFVVDARAAFPIFLFLMHIRVWTGV
                     LVLVSAIFFGIIEHYGFTVPVFLRWLRSFLAGSVRSARPWWR"
     gene            complement(29671..29883)
                     /locus_tag="U6309_000786"
     CDS             complement(29671..29883)
                     /locus_tag="U6309_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504240.1"
                     /translation="MDIHSTYKMLAKMQGNKELSSHRIAETEQAEADTVPQLKLQAEP
                     EAKPDMQNILSNMLGISSNKKHSPMD"
BASE COUNT         9125 a         6511 c         5294 g         9082 t
ORIGIN      
        1 tttggaatgg gtaaaaacaa gtagtgaaaa taatacactt aactacccgt taactcactt
       61 ccactctgat taatacttgc cctggagtca ctttgtctcc cttttgacat aatatttctg
      121 caaccacccc atttgcaggt gcttttattt ctgtctccat tttcattgct tcaataacca
      181 gtacagcctg cccggccttg acttcttcac cagcagaaac atgaattgca ataatagaac
      241 cggggatagc tacagtaata tctccaggtc ctatcttgtt attaacatat gaacgctcta
      301 ttttgtcatg aagttcagaa tgttgcacaa caacttcttc aggaacacca tcaacccaca
      361 aaaaacagga ttgttgacca tgctccatca ttccataacc agctactttt acatgataac
      421 tctcaccatg aagaataata tcaaactcag acattacgga attatctggt gcacttgatt
      481 gagtcaataa tggctcagga attaattgat tatttttacg ctgctctaag aattggcgcc
      541 caatttcggg aaacattgca taaagaagaa cgtcctcatc acttagtgcc aaatcactta
      601 tttcattttg caattgatct aattcattag gtaacaaatc gccaggcctg acttcaatca
      661 cttctgttcg tccaatcgct tttttccgta aagcagaact aattttgcca ggaggagtgc
      721 catattttcc ctgacagtag agtttcactt cattggttat tgttttatac cgttctcccg
      781 ttagaacatt aataactgcc tgagttccaa caacttgaga tgttggcgta accaaaggcg
      841 gataacccaa gtccttacga acgcggggga tttccttgtg aacagcatcc atcttgtcta
      901 atgcattctg ctcctttaac tgattataca gatttgagat catgccacct ggcacttgat
      961 acaattgaac acgagggtca atattctgtg cctcactttc aaattgagaa tacttcttgc
     1021 ggacagcttt aaaataatcg tctatttcca ataaaatatt taaatccaat tccgtgtcat
     1081 acggagtgtc agtcaatgcg gcaaccaatg cttctgtagg tggatgagat gaaccgccag
     1141 aaaaagatga aatcgctgta tcaatgttat tacaacccgc taaaacagcc tcataatggc
     1201 atatacttgc caaaccggaa gtagaatgac tatgcaaatg tacaggtaag ccagtcgctt
     1261 gttttaaacc ggcatacaat tcgacagtca ccgttggggt tagcaaacct gccatatctt
     1321 ttatggcgat actatcacat cccatttcag ccaatttttt ccctagctcc aaaaaattgt
     1381 ccaaagtatg aaccgggctg gttgtgtaac aaattgctcc ctgggcatgt tttttatgtg
     1441 atttaatcgc gtctatggct actttaaggt ttcgcgcgtc atttaaagca tcaaacacac
     1501 gaaatacatc cactccatta ttcacggcca atttaacaaa cgctcggact acatcatctg
     1561 cataattcct atagcctaac aaattttgac ctcttaacag catagagagt tgagtattcg
     1621 gtaacacttg gcgaagttga cgcaatcttg accagggatc ttcctttaaa aatcgcaaac
     1681 aggcatcaaa agtagcgcct ccccagactt ccatagccca aaatcctaca tcatccattt
     1741 tattacagat tggcaacata tcttcagtac gcattcttgt agcgattaag cattgatggg
     1801 catcacgcaa ggtgacatct gttataaacg ttttagacat aagctcttcc tggctcaaga
     1861 aataaataaa aatatcataa tcctaaaatc ggtacactct ttaagtacaa tccattgctt
     1921 tttactaaat ataaaatttc ttgttaaact tgcggagatc ttgccgatat cctcgcagat
     1981 atatagacat gttcataaag ttcaggcaaa atctgcaaat ctcagactat actgatgtgt
     2041 aatgaaaaaa attctagtat tacatggccc taatctaaac ctcttaggct cccgagaacc
     2101 gtccatttat ggacatgctt cgttaactca aatcaatggt gatttaatcc aggaagcaga
     2161 caatgcaggg attcgactca gctgctttca aagcaatgct gaagcagagt taattcaagc
     2221 tgtccatcag gctggaattg ataaaatcaa ttacattatc ataaatcctg cagcatttac
     2281 tcacacaagc attgctctta gagatgcttt aagtgcggtt gccatcccat ttattgaggt
     2341 tcatttaagt aacatttttt cccgtgaaac ttttcgtcac cattcctatt tctcagatat
     2401 tgccgttggt gtcattagcg gtttggggac gaaagggtat ttactggcct tacaagcgat
     2461 tatcaaagaa ttaaagtaga gagaaaaaac aaatggatat cagaaaaatc agaaaactta
     2521 ttgaactcct ggaagaaact ggtatttcag agattgaaat caaagaagga gaagagtcgt
     2581 taagactaag tcggcatagt aatatagtag cagaaacagc tccacaaatt cattatgtgg
     2641 ctccttctgc tgtgaagcaa acggcctctg ctccttcctc caatacttcc accaccgaac
     2701 ctcatgtgat tgaaaataaa caagttactt cagcctcctc aggtcataaa attcgttcac
     2761 caatggttgg aacaatgtac acagcgccat ctccagaaag cccacctttt gtaacagtgg
     2821 gacaatctgt taaagcagga gatactctat gcattattga agcaatgaaa atgttcaatg
     2881 aaattgaagc cgatcgtgct gggaaaattg ttgaaatctt ggtaaaaaac ggggatcctg
     2941 ttgaatatga ccaggccttg tttattatag aatagagagg cctgtttatg ctcagtaaaa
     3001 tagttattgc taatcgaggc gaaattgcgc ttcgtatact gcgtgcttgt aaagaacttg
     3061 gcattcagac tgttgctgtt cactctgatg tagacaaaga tttattgcac gttcgcctgg
     3121 ctgatgaaac cgtatgcata ggccctgcac ctgcccaaaa aagctatctc aatattcctg
     3181 caattatatc tgcggcagaa attactgatg cagtagcgat acatccaggt tatgggtttt
     3241 tatcagaaaa tgccgatttt gcagatattg ttgagcaaag cggtttccgt tttattggcc
     3301 cacgcggtga ctccattcgt cttatggggg ataaagtttc tgccattgct gccatgaaag
     3361 cggcaggagt accctgtgtt cccggttcag acggtccttt aggagatgac gacaacaaaa
     3421 atctcgagtt ggcgcgaaaa attggttatc caatcattat caaagcagcc ggcggtggtg
     3481 gtggacgtgg catgagagtc gttcatagcg aatccaattt acttagtgct attgcgttaa
     3541 cccgaagcga agcaaaagcc gctttcaata acccgatagt ttacatggaa aaatttctgg
     3601 aaaatcctcg ccatgttgaa ttccaagtcc ttggtgatgg gaaaggacat gctatacatt
     3661 tgggtgagcg tgattgctcg atgcaaagac gacatcaaaa agttgtagaa gaagccccgg
     3721 ctccaggaat cactcctgaa ttaagagaag aaattggcgc gtcagtgatt aaagcttgca
     3781 aagagttaca ataccgaggt gctggaacct ttgaattttt atttcaagat ggttgttttt
     3841 atttcatcga aatgaatacg cgcattcagg ttgagcatcc agtaactgaa atgatcactg
     3901 gtattgattt aattaaggaa caaatcaaaa ttgccagcga tattcctctg acattaaccc
     3961 aaaaagatat taaattacgc ggccatgcta ttgaatgccg aatcaatgcg gaagatccga
     4021 ggaattttat gccctctcct ggaaccataa aattacttca tcaaccagga ggcccaggca
     4081 ttcgttttga ttctcacatc tacagcagct atgttgttcc acctaattac gattccatga
     4141 taggaaaatt aataagttat ggggcaacaa gagcagaggc gattgccagg atgcgtaatg
     4201 ctcttgatga aattatcatt gatggcataa aaacaaatat tgaattacac caacgtattc
     4261 ttcatgacaa atcttttatg gaaggtggca caaacattca ctatctagaa aaaatgctca
     4321 aggattcata gcgtgtggtt tcaactaaaa atagaacatt gtcctaatga caagattgaa
     4381 gaaataaccg aggaactcga agaatacggt gccttgtcca tcacactcac agacaaaaat
     4441 gacaaccccg ttcttgaacc agagccaggg acaacgcctt tatggcctga agtaatcatt
     4501 catgccctat ttgcccaagc agaagaggca caatacgcaa gagaacaatt ggttgctaaa
     4561 agaccatctc tgcattgcag tttggaactg ctcgctgata aaaattggga aagagcctgg
     4621 atggatgatt ttcgacctca acgttttggt aaccgcttat gggtctgtcc tacctggctc
     4681 cctcccccgg aacctgatgc agttaattta atgttagatc ctgggttagc atttggtaca
     4741 ggcacacatg cgacaacctc cctatgcctg acctggctgg agcaggctga tttaaaaaat
     4801 aaatcaataa tagattatgg ttgtggttca gggatactct cgcttgcggc aatcaagtta
     4861 ggagctaaac atgtctatgc tgttgacata gataatcaag cgttacaagc cactcaaagt
     4921 aatgcgcatg ccaatcatat cactgagagc caactctcta tcagctcccc agaagcattg
     4981 caaaatcctg tgcatttaat tatcgcaaat attttgttgg ccccattaat cagtttaaag
     5041 gaacgttttc atcaactgct cccatcaggc gcgcatttgg taacatcagg aattctggaa
     5101 gaacaagctc ctttgctgat tgacgcttat gattctgcat tcactcatat tgcaactgaa
     5161 tattgtgagg gatggtcgtt gctggttttt accagcaaat aataactata tcactagggt
     5221 atatgtaaat ggcgaaaaaa caaatttact cttctttcaa gccacgtcga gcactgctga
     5281 gtgtttctga taaaagagga ataagagaac tcggtcaggc cttacatgac catggagtgg
     5341 aattgatagc cacaggcaac acagctgcga tattaagaga gcataaatta ccagttaccg
     5401 atgtcagtga gtgcacggga tttcccgaaa taatggatgg aagagtcaaa acccttcatc
     5461 cagctattca tgccggttta ttagcccgcg gagaacaaga cagccctgtt ttaaaacaac
     5521 atggaataaa acctattgat ttacttgttg tcaatttata cccctttgaa caagtcatta
     5581 accacccgga ctgcgatttt aataaggcca ttgaaaacat tgacattggt ggaccagcaa
     5641 tggtgcgagc agcagctaaa aatcatgctc atacttatgt catcgttgat cccaatgatt
     5701 actcaaaatt aattcattat ttacaagatc agaaagcacc ttcccattgg aattttgcgt
     5761 tagctaaaaa agcatttgcc catactgcag cctacgatgc cgcaattgca aattatttaa
     5821 ctactttgga taatgattat gtgccaactg gatttcccga catactaacc tgccaattta
     5881 ataaaattac tgatcttcgt tatggtgaaa atccccatca gcaagctatt ttttatgctg
     5941 ataaaaattc acatccaggc tctctaggta cagcagcctt attacaggga aaacaattat
     6001 cctataataa tatccttgat gccgatgcag ctcttgattg tgtgaaatca ttttccaatg
     6061 aaaagtcggt ttgcgtcatt gtaaaacata ccaacccctg tggcattgcg ctatccgaca
     6121 catcacttga tgcttactta aaagcctttc aaagtgatcc gatatccgct tatggtggaa
     6181 ttattgcttt caatgggact ctggatagcg atacggccaa agcaatatta gaaaagcaat
     6241 ttgttgaagt gattattgcg cctgatgcca atgaagaagc aaaaaaaatt ctggcagcca
     6301 aagagaatat tcgtgttctt ctaaccggtt tttggcaaca aggtaacaat tttagattaa
     6361 gtatgaagaa agtcgatggt ggtctattag tacaagaaca cgattctctt tctctggaat
     6421 catgtgagtt acaaaccgtt acccaagtaa aacccactga tgaacaacta caaaatttaa
     6481 tgttcgcttg gttagcagcc aagcatgtca aatccaatgc cattgtatac gctaatgatt
     6541 tggctactat agggattggc ggaggacaaa ccagtcgagt aatgagtgct cgtattggct
     6601 tgtggcaagc agagcaaatg gggtttgatc ccaaaggcgc tgttatggcc tcagacgcat
     6661 ttatcccttt ccctgatact attgaaatag ctgcgaaggc tggtatatcc gcaattattc
     6721 aaccaggggg ttctatcaga gatgaaaaaa ttatttcttg cgccgaccaa cacaacatag
     6781 cgatgatttt tacaggagtg cgacatttta aacattaatg tcaagcatgt attaattcca
     6841 agcttgacta acttgaacaa agagtcgtat ataacgtatc atcttctttt taattgaagc
     6901 tttttgatta gcataaatgg accttttctt gtaggactga tggacttgat attagaacaa
     6961 ggagcaagta tttctagaag cagattttac ctatagaata aataagaatt ataggaatac
     7021 ttactaatca actggtaatc acgcaaaaga caaaaatgct actgaaagtc taatgcgtca
     7081 ctgaaaccca tggagtaata taatgacaac tgagcaatac ccgtcctcac ttgtcaatcg
     7141 tcttgcaatc acagagcctg cgttggttcc aagtggatat tatcgttcga aattatttat
     7201 tgccccattt tctacaaatg ttttagttgc agcggccgga ccaatactgt ctttattgga
     7261 aagactttgt ttaagtactt ctttacctcc agtagaggat ataagagaaa atatacagca
     7321 tgaattacgc gcctttcaca gtaaattaga cgcttctaaa tatccgttgg atattattag
     7381 tattgctcaa tatttattat cagctaccat tgatgaaata ttaggtaaaa gttacctaag
     7441 ggtctataac ctgacgacag agttcaaatc attcactccc ttatcaagtg atggggcaca
     7501 accccagcag cgcttttttg aaatattgaa ttacattaaa gagaggccaa atcaatttct
     7561 cgatttaatt gaattagcct acttttgcct cattgccgga tttgagggtg agtaccatct
     7621 gaaagcagac gggcgacaat gtcttgataa tactattgaa gatttatatc aaatcataca
     7681 aaaataccgt tttaataaac cacatcgctt gtttaatgag aatcctcttc ccaaaactat
     7741 taaaaaaaat tacaaagcaa cgtggataac aactattgct gctgtaggta ttgtaattct
     7801 agcttttgtt actagccatg ttttattaga aaacaaagct aaaactgttt tatttggaca
     7861 tacccaactt gctatgctgg aaaactaatg gacaattcat tacgcgcatt atgtgatgct
     7921 ataaaaaaaa ttctctctca attaaaacca caagctaatc aattatcttt tattgtcgtc
     7981 accggaaaaa acgcccaagg caaatcggca ctgttaaagc aaagtaatat ggaagaaatg
     8041 cccgtattca gcgaagagca tgccaaactg tattttaatc aaaagggaat catagtagag
     8101 cttggtgaaa attggcttac caatagcaaa acattattgc tcacaacact taaacaatta
     8161 aataaatgca atcgtcatct caaaattaca ggcttgattt tatgtgtgga tgtcaatgat
     8221 ttactgatcg ctgaaccagc acaattcacc gaacaaaaaa aatctcattt gcaacttatt
     8281 caacgtttag gtgttaacct tggatatcag gcagaattag ctttaatatt caccaaaatg
     8341 gataccttgg ctggcttttc tgaattttat caaatggatc atgtcaatga tctatccaaa
     8401 cctcttggct tttcccttga ttgtgtcaac caattaagta aaaaaattga aacattttcc
     8461 cttcaattca atcaattaat tgaagctttg ggacagcaag ttatcaataa aatgcacccg
     8521 gcacgttcca ctattaagcg cagtttgata cgcgagtttc ctttgcaatt ggccagcttg
     8581 agagctccaa ttcagtcgtt aattcagggg atttcgccaa aattattcca tgttcactct
     8641 atttttttca cgagtgccga gcaaggtgga gtcagtgtag ataggctaaa caaaaaaatt
     8701 cagcatgaat acgctttggt tgttcaggat actttccctc aagcgaccaa tttcagatcc
     8761 tattttgtag aaggtgcact gcaaacaatc caggaacaat gtatccaggt accacagact
     8821 acaaaatttt ctcaaaaacc attcattgct gttgcagtaa gtttggccgg gctcagttta
     8881 ttaacactga tctataacca ctacaaaacc tctcatattc ttgatgaggc tagtaaagaa
     8941 ttacttgcct atgaagcact gaataatcag ggcactagtg gcgcacaagc gctatatcac
     9001 ttatcgcagg ctgcaaataa aatgaatcac attacatcaa actccatatc actgcctact
     9061 gtgcaccaat taaaattaaa ccttcacaac aatgcacaaa atcgtttaca aggagaattt
     9121 cttccctcct tagccaatga gttagaacaa gtcatcacaa gtcctggcaa cacccctatc
     9181 actcgttata aggctttaaa aatttattta atgttaggag actcaaaata tttttcatcc
     9241 caacccattc tggattggtt tcaacaacaa tggcagggac gtgcaccagg cactacgcaa
     9301 aaacagttgg ctcttttaaa acaaactctt agcaatcctc ctcaaaatat acccatcaaa
     9361 tcacaaataa tcagtgatgc gaggaattat cttaatgcac ttccaacaag ctatttatat
     9421 tactctattg cgaaagaata tttccctcag gaaaaacaaa agattgtgat cgaaggtttt
     9481 tctataccca cggatgaagt gccaacctat tttactaaaa ccggatttac gaaaattatt
     9541 aacgaattac ctaaaattac cagcacactt caatcagaaa actgggtact tgcgagacag
     9601 gatttgtctc aattacacaa tatgctgatt caggcttatt gttttgatta cgtcacttgg
     9661 tggcaaacgt tcatgaagaa atcccaacca ttacgttatc aggattatca gcaaggccgt
     9721 caattaacaa aattaataca acaatccaat tcgttttata aattcgctaa tcttattcag
     9781 caggaaacca aaccggattt aaccgaaggc atatccacgt ttaatcaatt cattgccaat
     9841 caatttactg atttaaattt aacaacttta tccagcatca aggaattgga ttttagaatt
     9901 tctgaattag aacgatttat tgcaacttta tctgttgtaa atgatggagg taaaacagcg
     9961 tttaccatta cacgctcgcg ttttctcaat gaaaattcct ctgaccctgt gagtttgctc
    10021 tacagccagg cacgacaatt acctgaacca ttgagttcgt ggacaaagca aatagctggt
    10081 gacacttggg ttatactgat tagagatagt cgtcaataca ttaataatca atggcaaaaa
    10141 accgtctata gagagttcca aaactctatt gcccagcgct atccttttga ctcgtcacaa
    10201 actgcagaaa tagatattgc agattttaat cgctttttct caacacatgg cttattgaac
    10261 acctactctg aacaatacat taagccattt cttgacactt catctccaga atggaagcct
    10321 aaagctgtga atgatttcat cttgcctatt tcaaatgaag taattgatga gttaattcgt
    10381 gccaatatca ttactaatat gttctttcct gatcacagtg atgaaagcaa aattgatttt
    10441 agcttgcaaa aaatctctct ggatcctgta gtttctaacc tagtccttga aattggcggc
    10501 actaaattga tggataatca aacaagcgat tcactaattc gcttcacctg gcctcaaaac
    10561 aacgctaaat tggctcttga ttctattgaa gggaatcatt acgaactcgc agaatatgga
    10621 acctgggcaa tatttaaatt acttgaaaaa gttaatgtgc ttgtggatga acaagatagt
    10681 tccagcctcc aaatactttt tgaagtgaat agcaactccg gccgctattt attaaaaaca
    10741 aacaaccaaa ttaatccttt tactccagga attctaaatg ggtttacctt aaacgatcat
    10801 attgcataat tatgaagcaa aataccaaac acagcattat tgctggatta tacggaaatg
    10861 ctttggagtg gtatgatttc ttgctctatg ccagttttgc ccctgttttt gctgaaattt
    10921 ttttcccttc aaaaatccat tttgtttctc ttatagcaac atttagctta tttgctatag
    10981 gattcatcat gcgcccaatt ggtgggcttt tgcttgggca ctatgctgat catgtaggac
    11041 ggcggaaagc attaatacta tctatgtcag taatgaccat ttctaccgca ttaattgcgg
    11101 tattacctga ttttaattcg tggggaatta tcgccccact tttattttct attttacggc
    11161 tcattcaggg tcttgcagtg ggaggcgaac tgcctggctc gaccacctat ttgattgaac
    11221 atatgtttca acaaagccgg gggctcgctg gaagcctcgt gttaagtagc gcttttttag
    11281 gtatttttat tggagcgctt accgcatcaa tattcagcac attatttacg ggtgatgctc
    11341 tattaaattg ggggtggagg tatgcatatc tgttaggagg tattttagga attttgggca
    11401 tttatttgcg tattaaaagc tttgaatcac ccacatttct aaaggataag catactgctg
    11461 aacttcctgc aaaagtggta ttcacgcgat ttaaacagca actgttttta tctgtgataa
    11521 ttactagcat tcttgctatg tctaattatg tacttatcgc ctatgtcaca acttttttag
    11581 tcaaatcaga gtattttctc ttgaaagatg cgttattaat caacttaatt gcccttgctc
    11641 ttttgacagt tttaataccg ctgatgggat ttctttctga tgttgtggga cgcaagccta
    11701 ttttcttaat cggtttattt tctctttgta tattaatctt cccatttttt tggctcttat
    11761 taagtggtag ttggtggaaa gcattatgca gcgaattatt actagcgata gctttagccc
    11821 caatcaatgc gacagtaccc actattattg cagaaatgtt tccaaccagt gtacgggcga
    11881 gtgggatttc catatcctat aacattggcc aggctttgtt tggcggtacc atgcctttgg
    11941 ttgcgtttac cctgattgaa ttaagtagca ataaaatggc tccggcttgg tacctgttcc
    12001 tatggatcct tatcgtgata ccaataacta tttccattcg tgaaagctat caaaaagaac
    12061 ttacttaaaa cattagattg agataaaccg acataatttc tatcgattga ataagcaaca
    12121 atagatttaa atcagttttt tagcagaaaa aatccagcat taatagccag aaaataagtg
    12181 cattgagacg tactaataaa aagcttacct gcttcaacct acttttgcag gcaagctttt
    12241 cacgttggga tcttttgaat aagcatcaag aatatcattc accaattggt taatcattcc
    12301 ctctctctca tcgtcttcaa ttaacccaac tttttgcttc atgcttgcta atcgagcctc
    12361 caattcttta gtcactgtac catttgggaa cagtgcagct aaaagccgtc gagcttcact
    12421 tggtcccaga tgcctataca atagatttcg tactgtggca tcttctgcag tagtactaaa
    12481 tgcccataac tccaccggcc ccaatgttaa ggttaataat tgtacgttga tgcctgtttt
    12541 agtagcaaac tgagcaagga aagtagcgcc accttgtcta ggaccgtgaa ctcgagtacg
    12601 taaagcaatc ttggctgttt cagaaagtcc aaaaatttga ctggttttct cgacagcttg
    12661 agatggtcct gcatccataa cataaatagc ggtagcaaaa tcaatcatta ccggatcaaa
    12721 gtcatctact gcttgtgata ataaggaaat ttgcactttc cattttcggc cttcacgcat
    12781 atcaataata acttgctcac gaacagctga cgattttgcc gtgcgatgga attcatcata
    12841 aacaatacgc ttatgatctt ctcgaatctc taatactctc tgtttgtgat actctttata
    12901 ttgttccggt acattattca aactttcttc tgttaaataa tagtgtcttg ccaatacata
    12961 tctggctaac atatacataa ccgctgtttg tctatctgcc gcatcaccac cacttttcgc
    13021 tacttcatcc aaatcaagag acactactct tgcgtcacca atatcaaaac tagtgacccg
    13081 tgataaaata ggatattcgc gaacagccga tgaaatcatt cttgaaaaag cattaattag
    13141 tgattcacca gttggagctg taatcttttc atacaaatcc tcaattgatg gtgttctaca
    13201 gatagaagca gcatccgcta ataacggcat tgcatatctt tgcgccaaca tcgcttcatg
    13261 gacaaaacct gctgaataga gcgagtcagt tacttcccac caggttgatt tggcatctcg
    13321 gacaaaacct atttcctcca ggatgctatc gataaattcc tctattcctg gagcataagg
    13381 agtcggattg aattcatcag ccatgctttt atataattca tctaccacca tacccgccaa
    13441 atcggccata ccgtcatatg gctttgcagc acctaatggc gtagttagca aggtcatgaa
    13501 gttgactaag aatgatcgtt ccaaagcagt tggataacga catcccaatt gggtatcaaa
    13561 gggattaatg gaataatctg gggtcatcct taatctatga taagcaacca aatgacgttt
    13621 ggatgcagga agagcctctt ttagcaatga aatcaaacca ctacttgagg gacctatatc
    13681 aataattgca atgcgtggca aacgcattaa accacctgaa agacaaagag ctaaattcag
    13741 tgcatttgac aacacagatt taccagaacc aggacgagcg taaaccagat caatccaggt
    13801 agtttgttca gttgagcctg gttgaaatgg ccacggttta ccatctggtg ttctaaaaag
    13861 taaggcacct gttttccagg gagatgctgg tcttgtaata ggcagcattg agataacatc
    13921 tgacaaagga gctactgatg ctactgctga gctgttcaaa gtagtagcta gcatacttga
    13981 cacaaagcct gcaaacggat ccccacaaat ctctgataca tcagtagaac cccaaccttc
    14041 aatagccttt actaactcag agcttcttcg ccgcaacaag ggaatattgc cttcaggggc
    14101 ccaggttgtt gcaacgacgc gtagtctgac aatcgcctca tccgtattga gttgtatata
    14161 tttcagcaga ttaaccgaat cactaattaa acgattctgt gcggagctaa agctcaatat
    14221 cgctgccaag agccccttca atttaattgt gcttaatcct tcactttcta acaaaaaaga
    14281 aattttccag ggcacatgtg aaggaagaat acgagaaaat aaactaataa atggtcttac
    14341 atcctttgga aacaaatcaa tataggttga agcataaatt ttatcgccca ctcggacagt
    14401 gcgtaaatca ataatctctg catctcttgg caaaacctgc ttggacaacg gcggccataa
    14461 taaatcagaa ggatccccct caaaattatt cagctcgcga acaggtattt tatctccagg
    14521 taaagttgcg cgccaatcat ctgcagtata atcaggatca cctgtcatgc gaatggcatg
    14581 tactgcatcg tgtacctcaa gcaacttagc tatcacattc agggcatcca aatcatttag
    14641 aattgcacgc acataagcat catgtgtatc tcttatttcc ggaacagccg caaaaatcgt
    14701 ttgtgtgttt ttaaatgggg gagcttttgt atccttgatc atcttcattt ttgctttgtt
    14761 cgcagctttt aactgatcgc tcgccaagtt aaatggtctt gtatacaaaa caaaataaac
    14821 acgctcctct gcacaatatt gtgataaata attgacccgc tcctcaaata aatctcttaa
    14881 attaagttca aggcgacttg ctgttgcttc cgcagggtca taaatatcct tgatcatttt
    14941 tactatgttt tgcttatcat ggctaaaata aacttgtagc gcatgaccag gtctacccat
    15001 agcagcttga aatgagttgg ctaaaccttc aacgagtcgt tcaaactctt cagggcctgc
    15061 caatccagta atcccttcaa tttttaatat cgacagtaat gatccatcat gattaactaa
    15121 tacggtaggg ctatccgccg tttctaaatc tatataagac tctgtagttt gcttcaagga
    15181 agtacttaac caggcaaaga aagtatcgac gccttcaaaa aacgattctg accaatttgc
    15241 catcctttgt cctcaattat taatagtact tttactgttt aatctgcaat ttcttctaaa
    15301 gcactggcag cccatttctc tatttgcttc ctgaattttg ccctagaggg taatagtctt
    15361 atatttttaa aaagtttctc tcttatttct tcactattca caccagaatc aatcattaat
    15421 tgcccaaata ttgcagggtc acttgtaccc tcaccaaatt tttcttcaac aatttgcgag
    15481 cgaaatttaa aacttctata aatattctgt gcactggatt tgtatccggt atcattggta
    15541 atagcacaaa aaagaacatg gcataagcta tttagttcag cttgggttaa ttctggcagg
    15601 tagatcaaag tacctccacc ataaccacca actcctactg actcaacaaa aaagcattgc
    15661 gcacaaaaac aacaagctgt aactaaattt gataacctat tgtttgtata atcaccatcc
    15721 agattcacga tgtcttggta taatcgggct tgaaacccgc aaaattgaca agtataccta
    15781 tctcgctgaa agattttttg ctcataggat ttgaatcgct catcaatttt tctggcagaa
    15841 tataaacgcc aggaacctgg tgaagcaatt agttttagct cacatcgttg ttgattatcc
    15901 gccatatttt ggttcacatt cagtcaattt tcaaaaaatt atgtgttcta tccttagcta
    15961 ctggctaagc cagcttaaag atatttgacc atattaatag agacaatcat ttaaatttac
    16021 tatttaacac aataaaaagc ctataaatta aattgggtaa agcggtattc tctttaccca
    16081 aataatataa ataatctctc tattaattag tcttatatat tgttccagta ggtccagcag
    16141 ttttaccgcc gctaccaccg aacatagtta ctccggccac tcctaaaata gaaggaagga
    16201 ataataatgc cgcagcgata aatatcaaag ctattggtgt tccaattgga atttgagtag
    16261 gattatcctt gtgctgctta aatttcataa tagcaccaat agaaaaaccc aatccagcca
    16321 agtaagagcc cgctgtaatc aatttagtaa gacttgtaaa tgaacccgta atttgtgatg
    16381 ccattttacc taacgataca cctgctgcag catcctgaca aattaaagtc aataaagcca
    16441 aacaagctgc gcttactagc cagtgtttat aacctgattt atggataacc ttaatgttca
    16501 caattctctc ctgtttttaa attagcttaa gtaccatata aagtattgtt aatcatatca
    16561 actgtaccaa caatattgat tgccaatatt cccccaaata catgaatcaa acctttacct
    16621 gtacctcctg gaggttgccc ctgcgacgcg gaacgtgcaa ttaataccca ccctcttaca
    16681 aatgcaatta gaccaataac ccggataatc atcaccaaag gtctgcctac tagactgcca
    16741 ctaccaaata aggctgaaat agtatcgctt ccactactca cacctcctga gtaggccaat
    16801 ggattgctat aaccaaaagt cgtttgcagt acggaggaaa ccaatgacgg gaaatagata
    16861 agtaatgcac ctaccatcaa atacatcacc ggctctttga tactcgtatt actcgacatc
    16921 attgtcctgg cttctccata aacctttaag ctataaattg ccttaaatat aaaagcacac
    16981 ccaatcaaat aagcggctcc agtaatcagc ctttgaacag gctccaactg attagcaata
    17041 ttagctaata tgttagcttg actcactatc cagcatgcta ctgtgccgcc agtacaagtc
    17101 ggatcagcca tatacgcctc aactatcttc ttgactaaac ttaattactt gtccagaact
    17161 tgttaaaata cgaccttgca aagaatcaat taatttaacc attccatatc ctggaatttt
    17221 tgatccctct ctaactgtaa gtgtagaacc atttgagcca attagccaag cccgacctgg
    17281 aattactgct tgtatgtaat aaataattct tgcttgaact gttggccgag acacttttac
    17341 aactttctta ggtgttgtac gtgccattaa gacattaatc acttccgatt gtctagcaat
    17401 ttgatttgac atattaccaa taatttgatt cagatttaca atttgggcat ttaaattttt
    17461 gatattatta tttactgcat tgatttgctc accaagggca ttgacttcag aacgaagact
    17521 ctgctgtgtc atttctattg ctgaaacttt cttctttaga tcggggtcat cttcaataat
    17581 agtcgtaggc tgaacttgtt gtatgggagt ggtagttggt attgtctgta caggctgtgg
    17641 tgtcgctacc tgtgtcaccg gaggaatagc gggttttgag gttacttggc tggatttatc
    17701 tgagaaaaac atccaaccaa ttattttata catcaccatt ataaatacaa ctgccccaat
    17761 agcaataaga gcatttcgct taatatcttt tttctttgtt aatccctctt ttccagattg
    17821 aaacgaagct tctgaatcga gatccgattc accagcctga tccatatcgt atgaatcaag
    17881 ttctgcaaat ttatattcat cattattttg atcgtgctct gccatcatta tccgccatca
    17941 aattaaattg ttgtaacatc ctgggtaaat aaaataccca aacctgtacc agaataaact
    18001 tccacagttg ttggtgtatt aaacaattgt tgcgcctgtt gactccatgc ctttccaacg
    18061 gtagccaatc ctataaccgc attttccaaa gttgagcgac caacaccatt ggctacagta
    18121 atattattac caccaccagt acccccaatg gtgattgttg tatttgccga ctggaatgca
    18181 tttccaaatc cttgtaagaa agaagaagca aaaagggaac catatcgcat taaatagtga
    18241 tgattagttc tactggctag ggctgttctt gccgtgtttg gatcaattgc ataagccgag
    18301 atggatatag ttttttctgc tcctggaatt gacattgtat taaaggtaat caccatcttg
    18361 tccgcattag acggtaagtt gaagcttcct attaattttg agccttttag cttaccagta
    18421 acaatagttg ccaatattgg acctggctca tcactattca cagcggtatc cagaaccgca
    18481 aacataatat cgcccgtttt gatgatagcc tgattctgag caccacttgc accttgctct
    18541 actggttgat tattagcacc tgcaacagtg cctgctcctg gaactgagct ttcaccacca
    18601 gatgtttttg tttcttctgt accttctgta tacacctgtg tttcaacctt cttccagtct
    18661 tgaaccagct gagtcgccgc tgtgagcatg tcagaagttc tttgttgaat ttcttgctga
    18721 tatttttgct cagccaattg ttcattttgc ttttgcaaaa tggcctgaag ctgctcagca
    18781 ctagaggcag catcactggt tgttggcctt ggagtagcgc caggaattga gggaattcct
    18841 gtaatacttg agtcgaattg ttgcaaatca ggcccttgta accctgcaac ggctgattct
    18901 tgtggagaaa atccagcatt tctcagctca gctgaggtat atccggcttt ttttagctca
    18961 tcagccgaat atccagcatt ctttaattct gaggcactaa atcctgcgtt tttcaaatct
    19021 gaggcactaa atcctgcatc tttcaattct tttgcgctaa acccggcatt tttcaaatct
    19081 gaggcgctaa acccggcatc cttcaactcc tttgcactaa atccggctgc ttttaattcc
    19141 gctgcagaga aaccagcatc ttttagatct tttgcagaaa acccagcagc ctttagctgg
    19201 gcagcagtga atccagccgc ttttaattca gcagcagaaa acccagcgtc ttttaaatct
    19261 tttgcactaa aacccacatc ttttaattcc tgggcagaga aacccgcatc acgcaattcc
    19321 tttgctgtaa aaccggcatc ttttaaatct ttcgcactaa aaccagctgc ttttaattca
    19381 gctgcagtaa acccggcgtc tttcagctct tttgcagtat aaccagctgc tttcaatgct
    19441 gcagcggaac atcctaatgt ttgctttatt gtcaaggcac taacaccagc tgccctcgct
    19501 ttttttaatg actctacgct acagtctgca acacggccgt ctgcaataat ggatgcagga
    19561 tttaatccag catcttccaa ctgctttggt gtaaatcctg ccgcaagcaa ttcttgaggt
    19621 gtaaatcctg ccgcaagcag ggattgcgca tcataccccg cagcttttaa agcttccgca
    19681 ctgcaaccat tgagttcttt gatgcgtttt gcagatacac ccgcagaaaa taattttttc
    19741 agtttatcag gatcacagcc agcagccttt atttccgcat cactcaaagg agttgctgca
    19801 ctgatttgag ctggtgtaaa tcctgcgctc gccaaatccg catcactgaa tccggcagct
    19861 ttgagggcct gtgcgctaca tcccgcgtat tgtctgatta aagtagcact aactcctgct
    19921 tccctttcct ttttcaaagc ctccacatca caaccagcat tcttcacatc ttgcggacta
    19981 attccaggcg gaagctctgc ctgagcggct ttgatttgag cattagaaaa tcctgccttt
    20041 gccagatcag ctggtgtaaa tcctgcgttt aacaaatctc ttgcagtaaa tccggcatct
    20101 tttaattctg cagcagaaaa acctgctcgt tttaagtccg cagcactaaa accggcatct
    20161 tttaactctt tagcggtata acctgccgct ttcaactgct cagcggtgca accacttatt
    20221 tttctaatcg cggacgcact aacccctgcc tgacgtaatt tggccaatgc agcagctcca
    20281 catcctgctt tgcgcacatc atctgcagtt ataccatctg gtaagccact tgcttttgca
    20341 atttcagcat cagagaaccc agctcctttt aattccccgt ctgaaaaacc tccatctttc
    20401 atttcttgag ctgtaaaacc cgcattacgt aattcacacg catcaaaccc acaatttcgc
    20461 agtcgacctg cactataacc tgcttctttc aactgccttg cattataacc agcggctttt
    20521 agctctttac actcacagac ctgctctaat tcttgcaaac cgtatccact atcttttaac
    20581 tgaacacagc tacaagccgc tttcaaatca gttaattgag ctccctgatt cacaactttc
    20641 gtaatcacag atttgctaca accaccatct tgtaactctt gtatccataa acttcgttga
    20701 ggaccaccta actcttcttg agccaaagca gcaaaaccca ctccactttg ggaaccgata
    20761 acaccaactc cttctcctag tgcttgggtg cggataattg ttggaatagc actcccacct
    20821 gttttttcag cgacttgtgc ctgggttata ttttgttctt cttgcaattt ggcatattga
    20881 gcagttggat ctaaaactcc tggaatagat tgaattcctc ccggcactgt agaaacttca
    20941 gctgcagcaa tcgaactggt agtagcgctt cttattttaa aaaagcctat tactaccgcg
    21001 atgattaata aagctgctgt aaaaatgata atcacccgag ttcgagtatt ggaaaacagt
    21061 gactttagat tttctttttt gctcgccatt tgcttataac ccttctacct tgagttgcat
    21121 gacttttcca tgccaagaca ctaataaaac cggagatttt tgcatttcat aagcatgcgt
    21181 tccatctgcg ctagtcatac tggctaacca gcccggcgac aatatagtga gattcgttct
    21241 aacatacatt ttttcattag ataaccaagc tcgagcatcc ccaccactaa ccactagacg
    21301 cctgcttcct ggcggaggaa caccttctaa cacatgaaga agtaaatcat tcgctgatgg
    21361 gggaatgcct tcttcagttg gcatactttt agcgttgggg ccatagcctt gaactctcaa
    21421 gtctactcga taatccacgg ctttctgccc gggtataaga gtaagcatga caggggtatt
    21481 taatcctctt aaccttactg ccaaattacc atagttatat agcttcgttg cttgaatcat
    21541 taaagtatta ctggttttat cccattgaat attaaacgaa gagggatcac ccaaatcata
    21601 tgcagcaata ggccaagggg ctcctgttga atccaggaac actaaggaag acacaaaacc
    21661 ttgtgataat ctaattaccg gcggtgttga gcctggagac agattaacaa attgtgatgt
    21721 cgctgtaggt ttaggtggag tgcctgccgt tgaagctttg gcatactcgg aagtttcata
    21781 aatttgtttt aatttgacta cttgctcagg atttaaggga tataagttgc gtgtcatatc
    21841 cttaaatgcc ttcttatcaa taacttctgc atcatcctga ctaattatct gcccatcacc
    21901 gcccgcattt gctgtttgat ttgcagccgg agcattagcc tgaccggatt gattaggctg
    21961 ttgcgttgaa tcagaagccg cattatcacc gccatcaccg gctcctgact gtgcatcagg
    22021 ggaagggttt tgtgataatt tctgttgtag cattcgtagt tgctgcaatg cttgttgagc
    22081 atcatctgat tgatcagccg cataggtact gcatgacata ctcaatgtca agcctatcac
    22141 caaacaatat ttacataatt gatcatattt cttcatcact aggatacccc accgctggca
    22201 ggtccaacaa caaattgtga aatacctatc cctctaggcg aattcaacgt tgaaactcta
    22261 gttattaaca ttgttactac gttattttgt tgtgtaaatt cactggcact ttgataggtg
    22321 actaatattg gcatttgtac cctccaggaa tacctaccat tgagcacacc tttttgcaaa
    22381 atgattgggg ctcgggttgc tactgctgac acaactaatt ttttcgcttt aaccgcatca
    22441 aggttattag attgctctaa tgcacctaaa aattggtccc agccttccgc agtaaaaaag
    22501 ccggaagaag cctgtagctc atcgcgataa tttacgaaat tataggtaaa tgcggcaatt
    22561 gctgcctgat tagcccattg tagtacagcg gagtccgatt ggtttggttc atccaaagga
    22621 aataaagggg taatacgacc gttaatactt gttgcaaagt actttggagc aggtggatga
    22681 gtaatcatat agacaacaag cgatgccaag accacattta ccacaatagc aatcaataac
    22741 gccaaaatga ctttgcgttg actgtcttta taaaacttat ttcttaatgc aactacagtt
    22801 aaggcatctt cagccataat atcctctaaa aattattgcg gtataatcct ttgtgaatct
    22861 tcatctggtg gatccggcgg aagcaatgct tcagatgaca tgttaggagt cgacattggc
    22921 ttaagctgga taggaggagt tatgccgctg gtcgcataaa aatctcgctc aggttcatgc
    22981 atatgaacat aataaatcaa cccagacaaa ccacaactca atccaagaga aagaattagc
    23041 agtgttaagc cacgtctgta aatatggaca ttaaaattct tatttcgttt tattaaagcc
    23101 catgtttctc gactcatttt tacctcaatt atgccacagc ccgcctaaat gtgatctcca
    23161 ccctggcatt aggtgatcta tcaccaccaa gagtataact agttataggt ttatcactac
    23221 ctaacccttg tgtaaagatg attctgctat caaccccctg agaccatagg tattcactta
    23281 ctactcttga tctcgccaat gtcaatgctc gttctctttt taccgacaca tatttactac
    23341 tgtaactggt taccgttata gcaattttac gaaattgttt aagaaacgcc gcgatctcat
    23401 ttaaaaggct ataggaagcc caatttaacc gtggagattg atcagcaaac agagcgctag
    23461 caggaatact aattaaataa ttttggccta ctgatgcaac tttaatccct tttttattta
    23521 aatcagtcat catttttatt attgttgcat cgcatgcacc atcaacacgg catggtaatt
    23581 tgggcacttc attctcaggg atataaccag aacgattaca agcactcagc aacaataaga
    23641 ataataatcc agttgtttta aacaatacat agatgtagtt agtacgaagt gatctcacct
    23701 gttatcccca tctttgaaaa tagactaatt gtgttatcta tgtctaacta tctcaaacat
    23761 tccatttgat aataaaaaca tacaaatcat tacgttacaa tgaatttaat aaaattgcca
    23821 tttttttaaa taatttattt atttaagatg gggttgacta atcaaggcaa atcaacatac
    23881 gccaaattaa tctatgagta aactgataat taccctgtta attcagaata caaaaagctc
    23941 tcccaaagag gaaccacaca gaatatcaac ctgatagatc aaggtttaaa gaaacaacgt
    24001 cactgcccgg attataaaaa actcagagta acttgcatat ggagctctct tcatgcccaa
    24061 tagcacagga acacttctac ctccaattgc cgactgagtc tttatgttaa ttcctctgca
    24121 gcctttttat tggctttctc acgttctgcg gaaattttag ctgataaatc tcttataata
    24181 ccagtaagtt cttggacatc aatcacatcc ctttcttcag gaggatagct agtcgctatt
    24241 tgaaaatctt tgattaattc attagctact gtaccagcgt atttgtcttt agcccctgcc
    24301 aaacgctcaa tcgttatcat ttgatttcgt gtttcattaa taggtaataa tggctcagag
    24361 aaaacttctt tgtcagcgac taatattgga ggagcattag ggtcaattcg tagtttgctg
    24421 aaaatagtta atgccccctc aacttcttcc tctacaatat cttccacagg ctcaggttca
    24481 ttttgaccat gaaatgcaat taaagcagca acgcctcgtt caattggctc tatgatagta
    24541 gattctttta atgccttact aatgagagtg atttcttcgt tctcgactgc cttattgata
    24601 ctcaagtcac cactttcaag aatgctctgg aaagaagcca attgtttctg tagcttcatt
    24661 aaataatcat ctggcggcgg ctctactttt aaaaattggt taattttcaa ttgcttaacc
    24721 ggtttgggat tggcataaaa catgcgagca cgaacaattt tggatttgaa aaatatatgc
    24781 gcctcacctt cggtttgctc ttttaaatcc aggagatcta cccgagctct cttttcaaaa
    24841 gaagagcttt tagtatccat atagctgttg gctatgctgg tttctttggt ttgaaacgaa
    24901 tccacttttg taacataagc ttcgccagct gttttagtga aaaaatccca ggtttcggta
    24961 ggatcttcca gtttcataca tattttaatg tttgtgtttg caccaatgga agctgcctct
    25021 tctttggatg ctttttggaa tgcaggtaag tcttgtccag caaaaatagc agaaaatcct
    25081 agagaacgag cttgcgcggg cactaccgca aatccttgta ctgcgtaata accgtactca
    25141 tcaagaatgc acatataagg agttggagca ttcgtgggtt ttcttagaat aacatcacga
    25201 taatcacctt ctacttcttc acctaaacct gccgccatca tagcttttaa tgatgaaaca
    25261 ataatcttgc cgaggttaga taattcatcc ggtgattttt ccagagcggg taataaaacc
    25321 actaatatac gtctgttgag caccacatct ttaaaatcta cttctgctaa attggtgcga
    25381 ataatatgac cataagtatc tgccagagaa gagaaacttc ttaccagctg catggtaata
    25441 aagccatgct gttccaaaac ttgtgatacc tgtttcccct ttttctcttt attataccca
    25501 ggcaaggtat ttaaatagtt tcttaaagga tcagttacca gttttggaat ggtttcaata
    25561 ttgacgcttt cttgatcatc tctgggaaaa actttatcaa ttacaataga ttctagtctt
    25621 tgcaaatcaa aataatttcg aatagtattc gcatcaagaa gaattgcccc ttcatcacgc
    25681 atatacacca ataaacgcat taatgcttct acgaaagcta tagcccgtcc cttccacata
    25741 tcaccatcag atgattggcc tgaagatccc atcaaactga ctaccaactg agttagcata
    25801 ctggaagaac cttggcaaaa tgggtttagc gtattggaaa gtcttttttc ctgcggacca
    25861 acaatatcgc gcgctccagt catgaagttg attaagagca aatcatcttc acgccccatg
    25921 ctccttacca tagaaaagac tttagcatac agagagttat ctcctttacc atcaacgtag
    25981 ataaacccgc tcccctgtac cagggcatta tatgacagag aaaccaaagt ttctgtttta
    26041 ccactacccg ttgaaccgaa aattaacgca tgggtacgca tatcatcgtt agcaaaccaa
    26101 agttcctcac cagtctttct gtcattacca aaaaaggtaa tacctcgagc tatattcggt
    26161 ttattaatac ccggtttcaa atcgttgtaa tccttcgcac gtgaaatttg aggcaatcga
    26221 aatggcaact tttgctttcg ggtatagcta taaagaaaaa atatgctccc cataatcaac
    26281 aaaaaggttg ctgcctcaga gaaataatat gaaacagctg ccaaagaaat aagaacaata
    26341 gaaatattgg tcggatctgc aaaaaaatct gccaaccgct gcgtaaaagt acgtgtatcc
    26401 ctaagtaaaa gggttggatc taattcatga cgagaatcaa taccccgcat catggctcta
    26461 attcctccat ttgacgtggt gttaatctca cttcttttac tgcaatttcc aaagctctaa
    26521 tcgcttcatc tatcattggc accaaagaac gtcttcccat ttccttttca gctttccaat
    26581 gtgcgaaagg acctgcaact tcagagtatg gagtttgcct gcctacacaa ttcaacatat
    26641 accacaaacg tctgtcgact ggtttgagcc ataaaaactc tgagctggga accacgccat
    26701 cttctcgtgc tgcttcaagc aaagaagcaa tcactgtgag cacataagca tgtttggcta
    26761 ctacttcctg aaccaactct gagttttggt attttttcat tactggcctt gcaacagaaa
    26821 aatcaggttt cccatccaca aatgttttat ctaaaacttt aagaatatta tttgctgcgt
    26881 ctctatctct attcattctt gccataaata cggcagacaa tgcgtaagcc tgaggagagc
    26941 aacgttcaaa accatcccaa taaggaccca attgcatggt aaatactcgt ttggcatcac
    27001 cacgccgaat ccccgctgtc atctcttggc caggcacagg attatccaat aaggcatcgt
    27061 cctttctcaa taaattatat tttcgcgcaa attccatagg tgttaaagcc attgcccaag
    27121 gccctttatt gacgtcttga ctaaccaaat cctccttaac tataggcatg atggcaggcc
    27181 aattaaattg ttcctgagcc cgcaaacttt tcatatcata ggttttgcga tattttaaag
    27241 tcacatttga gttataaaga acaaaggcta acactaccag gatacaaata accgggtaac
    27301 gcatataatc accgaccgca cgcatcacag tcaccatttg atcccaattg acagtgttag
    27361 gatcaagtgt ttgcatcaga taaatttgat tggccaagag ttgattattt aaaaacaaat
    27421 taactaatct tgcttgccag atattgatag taaatacaaa actaacaatg tactgatgag
    27481 ccagtgccca aacaaaataa gcagtaataa ataacagtat gacgatccat actggcgcca
    27541 tggaattatc tgaacctgat tgctgctgtt gttgtgccat ctctatacac aattagttaa
    27601 tagattctat atatcaagta tatactgctc agagctattt ttgcgagatt ttcgtgtact
    27661 atatcaaaat atgaatacaa ggatgattaa ataaagcgta aagacagtta gagtagaaat
    27721 aaatttttaa cacgctcgaa atcatgataa agaaaaccga aacggcctat gcatttttaa
    27781 ttagacggcc attggagtat cgatatacgc cactcacatg aacaaagcga ctgatgtttt
    27841 ccagactgat ggatttatct ttaagcgtta ataacggttt acctagttta tctaccggag
    27901 ccttatcagc agataaatgc aagatatctg attgactgat gtaaattgcc acatacgctt
    27961 cattttgttt attttctttt gtttttaata tcgcttttat gtcttcttca tctgcgtaaa
    28021 taggtcgtga aatcatttgc cggggcaaac tacccactat tttctcccag gactgaagat
    28081 tgcttccatc tgaggaatat aatgaaacat atatttcctg ctgaccgctc cttaaggcaa
    28141 ttcgatttgc aagatgagac tctgctttta acctggcctg actagcggca cctatccgtt
    28201 ttaaaaaacg atctcgaatt gcgattaact ttttaccaat tacccttaag aaattggatt
    28261 tatcccatgg acctttttca atagcatcgt ttaaggcctt tagaatggtt tctttttgtt
    28321 catccgagag ttgatctttc ataacttcaa tacttatggg aaccaagaat taggggaaat
    28381 ataaagaaag tataacagca tttgtaaaaa tcactgaaat gaaattaatc tttcaagatg
    28441 ataatttgaa accacgttcc tcatgacttg gttgctcaga cgatttttca gattcagcag
    28501 caccaaacat tctttctgtc tttgttgtta aaatcggttg atttaatgcg gctctctcta
    28561 cttttgtatt gagctctgtt aacacctcaa ctgcatccgt gttatccttc cctccgggat
    28621 taaagctaac agcctttgca acagtcaaaa tgcaatcttt taccggtgaa ataatttcac
    28681 tatagacact gccaagagtg gcatcagtta catcagattc acctatctct ttaacacgag
    28741 gtggcgtata aaaaccaaat ggatttcgtg cactgtcatc agtattatta cccattatta
    28801 ccactcctga gctaaatctc ttactttaca tatatcaaaa tatatctttc aatatatctt
    28861 ggtccgataa aactttctaa ttttttacaa acaactaatc atacattaac tcatccaggg
    28921 gagtataatc acggcttggg cccccttgaa taagctcatt cataacatcc gtcatacata
    28981 acaaacgcaa tacattcggt gtcacttcat caaaaaccac tctaatgcct aataacgctc
    29041 cttcagcctc atcataagtt gctcccaaac catagcctaa acacagagaa cacaattgat
    29101 ccgctctctt ggcaatagca ggcagcaaac ctgtttctga aaaaacttca tcaaaactta
    29161 caaatctatc gttcaggtaa aattttgcat tcatgcttgc tgcaatttta gccatacact
    29221 tgctaatatc tcgttccatt tatctccacc agggtctagc agatctaact gaaccagcta
    29281 aaaaacttct gagccagcgt aaaaaaaccg ggacagtgaa accataatgt tcaattatcc
    29341 caaaaaaaat tgcagagact aagaccaaaa cacctgtcca tacccttata tgcattagaa
    29401 ataaaaatat tgggaatgcc gcccttgcat caaccacaaa aaaccgagca cttctcgctg
    29461 agtcacgcca gtggctcgtt gctgaaaacc cacctgccat ttttaaacct caaaaaatca
    29521 tcctttaaaa aaagtatata cttaaaaaac agcttgtgca cgttgatact tttaagaaat
    29581 ttaaattaag attatttttt atgcaattga atattgcata cacgaagggg ttcaccacaa
    29641 atagatatcc taagtgttag taacccaaac ttaatccatt ggagaatgtt ttttgttaga
    29701 tgatatccct aacatattag ataaaatatt ttgcatatcg ggtttcgctt ctggttcagc
    29761 ttgaagtttt aactgtggaa cagtatccgc ttcagcttgc tcagtttcag caattctatg
    29821 actgcttagc tctttgttcc cttgcatttt agctaacatt ttgtaggtac tgtggatatc
    29881 catctgaaat ccccttttaa caactcttaa ttaagatatc acatacaaat taaggaatta
    29941 ttaagagttt gaaaaaaatc aaaaaaattc ggcactgacc gatcattccc cctttataaa
    30001 atttaagttt ct
//
LOCUS       DASLOE010000022        29605 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.022, whole genome shotgun sequence.
ACCESSION   DASLOE010000022 DASLOE010000000
VERSION     DASLOE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 29605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29605
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.022"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..436
                     /locus_tag="U6309_000787"
     CDS             <1..436
                     /locus_tag="U6309_000787"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504241.1"
                     /translation="VEKETNIKYKISAVGIANKEKPELFLPNPGYANSINISSVENVT
                     IIGKHNKVIIHNKYKELGEKIEELIDLVQNNADIPKKDKTDILALIKALESLFISETP
                     DIELVKKTDADLASKLKKWSATLGGNLLANGITSLITSYFSM"
     gene            complement(507..1313)
                     /locus_tag="U6309_000788"
     CDS             complement(507..1313)
                     /locus_tag="U6309_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN5504242.1"
                     /translation="MNVAWLTDIHLNFLKLEARKKFYQQVIETGADKILITGDIAEAK
                     DVCEILQEFSSYTNKLIYFVLGNHDYYFSSVNNVREKVRFLCAQNKNLIWLGEPKTLT
                     LADGIVLVGHDGWADARYGDFDHSLVNLNDSRLIAELYQAFLLNKSALKREMQKLADT
                     DAAVLHATLCDAIGTDIKKVIIATHIPPFPESSWHKDKPSDENWLPYFASKATGDVIM
                     AFAKKYTDIQFLVLCGHTHTAVSIELAKNLKIKSGHAEYYKPTIQKVIAL"
     gene            complement(1313..2023)
                     /locus_tag="U6309_000789"
     CDS             complement(1313..2023)
                     /locus_tag="U6309_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504243.1"
                     /translation="MNRSSAQKNIESLSLIAHRLGDLCDEVTFVGGCITGLLITDKAA
                     PDVRFTVDVDCIVNVITRPSYYAIAEKLRQKGFKELISGDHPICRWDCDGILIDVMPI
                     DKSVLGFSNKWYKEAQENSLKHSIFDNINIKVISAPYFLATKLEAFKDRGKQDFLLSH
                     DLEDIISVIDGRPEIASDVLVVSDNLKQYLSSEFGLLMSNDQFLQALPGHLNYSLESD
                     SRKKIVESRINAIIDVVK"
     gene            complement(2016..2564)
                     /locus_tag="U6309_000790"
     CDS             complement(2016..2564)
                     /locus_tag="U6309_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504244.1"
                     /translation="MIKSQDIVVLAKLLAYQTDKSWSQNSIAFELCLSPSQVNSAFKR
                     LVAVGLITPYHPPGKPQPIIQACEEFFLHGLKYVFPAKLGELARGIPTSYAAPSLSDQ
                     IVAGNDPIPVWPYGEGSERGLALKPLYSSVPESVAKHPDPLFYDLLTLIDAIRSGRVR
                     ERQIASQKILALLKPKKLVSNE"
     gene            2863..3495
                     /locus_tag="U6309_000791"
     CDS             2863..3495
                     /locus_tag="U6309_000791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504245.1"
                     /translation="MSIAKLCADHLRAISNNRGVKLKSGHAHELVASFFGYKSKAAML
                     ADTSSPIDRLNQAQFLVLTPPQFIEQRRKCLENLPSDLPDNSILGEELFTYLTLEKYF
                     SGRPFAAWKHLVDILATEYLLKHASSILSINFGLNEIAHSNFDKPTAEYIPVPDITGD
                     GVTLTAKMRYAENDINSQAIDIIVTIKLQRIAGHIGYANPNISVSTGGSI"
     gene            3543..4790
                     /locus_tag="U6309_000792"
     CDS             3543..4790
                     /locus_tag="U6309_000792"
                     /inference="COORDINATES: protein motif:HMM:NF017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504246.1"
                     /translation="MIAHIADVESGEKCNCICIECKSPLIAVKGQIKEHHFRHKSVTE
                     CEGGLESAIHLAAKQEIMKRKQITLPQCVSNASATDSRGIKHTEYKTIVQDKQIISFD
                     DVQEEKILHEMKADILASKGNTQLIIEIFYRHKVDEEKIKKIKKANISAIEINLSDLT
                     PEDVKSMETFWSRINNPICVQWLYNVKAHNCVSELANQLRAKIKKLEIEYAQEQIRKQ
                     KKEEKEKNHLLLALEDLKVLRSKQHAIQLNQEAEMHPAWKLHSQYLQLSFNTLPSFLN
                     LEVPDGDWIFGCDRRIWQTAFYSSFIQKNGKPFCIRRVDEWLNNKAKCEVPFTAQIVG
                     IYGRRYPELVPSDVSINLPSTWRTLQSYFKHLCDLGMLEFSPSEGGWGKDSWFRIINN
                     TPPGSVQIAIEKNLMKWNINKIQ"
     gene            4830..7307
                     /locus_tag="U6309_000793"
     CDS             4830..7307
                     /locus_tag="U6309_000793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504247.1"
                     /translation="MLTKHSISKPINITEYTRPIKILYIVPYKETRKNHLILDAVFYD
                     SYTRWSGAAVFVIPANKNGFLYEMYDEWFENLDPDFVCSYVELTQTFIKKISKLCNPI
                     LISVEDSKYTSKDSFRRYLKDWQRVALIQPLSSLSLVSATKQYFEGRELGKANKILVE
                     IQPNESNRFISDNFGCNGHQYSTTQTVLGLFNVVCLNPYAKDGYVSEGTELVKSYKDV
                     LSRIGSREINTFLDLSVSYSTAIPKVNINNNELAFNVFLGDTCIDRINFWNSRCLTQD
                     RKSSIILSKNQYSDDDFLKLFGEFLNSYNERYGHNNQREVEVRSLSINLDERKEFARK
                     LGNNTRCMVWTNEKSSREVSISRDNFGKLYTSVDVQSSHYQTTKKESNGYVANDPYHF
                     KYISPHYDFLKDGDWMVELEIEREKDLSRYDNVTHVWMLPIRENSSKIFTSNLSRVTK
                     NHIIALIPSRKDRHNFFKQDIMRSGASYQLSFPSDESYFSCIVTNYNTDYFPEDPRAK
                     LRHKSYERIGVSSKGKYMRGIIRKIGSLYKAYYSLTRKLWRDCIRKEAVKEDLKFASF
                     NGLLETALGDSKFRKKYQNKYNASNDQQVKSDIKKNFFACSNLLLQQKVIYKVHPWVC
                     DYCGTHHVCSVDNLKFENTCTVCGEIYHVPYSIEWRFQIDAMIINALHRNNGLTVLWM
                     IGYISTQDCRVSSFYYMPEVDLFNSYKDKERSAEIDLICVVDGLLYIGEAKQTAALFL
                     KKKKDKKDEEAKFIEIIKKIKPDVALLAFERYSDLEEDTDKVRTKLIASIERMKKICG
                     DVDIRYIVAEEVDEGFNEFLCQDFEIS"
     gene            complement(7355..7654)
                     /locus_tag="U6309_000794"
     CDS             complement(7355..7654)
                     /locus_tag="U6309_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN5504248.1"
                     /translation="MESPVPKRLKEARLAANISQKKLGIAAGMDEFSASARMNHYELG
                     RHAPDYSTLKRIAQALSVPTAYFYAEEDDLAAIIKWFNQLEISKKKSFLGMIDNY"
     gene            7861..8541
                     /locus_tag="U6309_000795"
     CDS             7861..8541
                     /locus_tag="U6309_000795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504249.1"
                     /translation="MIVVASLLLLGFLVYDAIRSSKNKNSLDLEKIFKSSSNLCMQFI
                     EMQIFHACTYHAYALKQEWDGDVIWSIVRSITTDIYNDGLFTEFLPLYYFGITFAATE
                     RVNLNKSSASYMQLQLALMSKVYNAEQAAINMHSIITSQAQAPIGGKPTHTSSTIVVG
                     HAQFDSFINYCSTKMNHTNVELLSLPATLLHYCIENDLNAKEAFAFYDFLLENHKDYL
                     INKINFYL"
     gene            complement(8561..8998)
                     /locus_tag="U6309_000796"
     CDS             complement(8561..8998)
                     /locus_tag="U6309_000796"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504250.1"
                     /translation="MTKFKKGQSGNPGGKKPGTLNKRTRLATLLEPYAEGLINKTVEL
                     AMDGDVNALRLCIERLIPKVTGQQIQIDLQGLDIERSTTLPIIGKKIIDATAKGALSV
                     QDGNQFMHLINTQKEFLAQEELAKRLDEIESHQKTGCFFDELE"
     gene            complement(9163..11247)
                     /locus_tag="U6309_000797"
     CDS             complement(9163..11247)
                     /locus_tag="U6309_000797"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504251.1"
                     /translation="MSISKERIEDVVPRLFSKQLNEGFKLEAIHTYTDANGEEFYHRV
                     RLKNPDTNEKIIRPLRYEGNAYVFGEPKFNKKKPLYNLPQIINRRNEPIYFCEGEWCV
                     DIFNQLGCLATTSGGANSISKADFEPIKDRELIVWPDNDEAGKRYANDAIKVFQGLGV
                     NIKLIDIEQLKLPPKGDVVDWMREHPNPTKEVIESLPYKKLNSTLIKSDKKSSISNDK
                     GFKDNDKPQSEAAYIVEFVIERAELFFDSNKETYAYDLLTNETRKLDGRQFKSWLVSN
                     YYEVHDKTPREHSIREAIETLKGLAQFKGERHDVFIRVAILNDAYYLDLSETGKNRAI
                     CISPGSWHVVDDHPIRFVRPDAMSPLPIPLKGGSINALWQVVNIPEDMQLLVLAWLID
                     CLRPETPFPLLKLVGEQGSAKSTTQEFLRQLIDPNSCNLRASPKTDEDVYIAGGMNWV
                     VSYENISYLPQKIQDAFCVLSTGGGYAKRKLYSNADETQIKVKRPIVLNGICASITSQ
                     DLIDRTISIETPLVEERAESNRLRNIYSDNHSQMLGALLDIFAEALSILPSINLPKSN
                     CPRLIEFANLGMAISEVIGCSSNNFLEAFNLNRTEAIARTIDASPVASAIIEMADDNL
                     ALPITMPLKHLLQRLEKYRPPGPDNWPKTPKGLGDGLRRAAPALRQMGISCRSLGKQG
                     SYVSWEIKSIKT"
     gene            complement(11247..11528)
                     /locus_tag="U6309_000798"
     CDS             complement(11247..11528)
                     /locus_tag="U6309_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504252.1"
                     /translation="MKKQNFNRERLPSPSKYYKGQGLNLSGGLEWKTALCPFHEDTKP
                     SLRVRLDEGCFCCMACGAKGGDVLAFHRKKYGLGFKDTAIELEAWEEEI"
     gene            complement(11509..11784)
                     /locus_tag="U6309_000799"
     CDS             complement(11509..11784)
                     /locus_tag="U6309_000799"
                     /inference="COORDINATES: protein motif:HMM:NF017717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HEN5504253.1"
                     /translation="MVSSLLRRPKVQSASGLSRSTLYLRISQGLWTKPVSLGGRSVAW
                     PENEITALNAARIAGLPNDEVRVLVKQLEASRSQIAKGVSSNEKAKL"
     gene            complement(11892..12431)
                     /locus_tag="U6309_000800"
     CDS             complement(11892..12431)
                     /locus_tag="U6309_000800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504254.1"
                     /translation="MNAVGEDGCIYSVWIQYDSWNLREGILLVLGFNPRASSVVGHEF
                     RGNENAKERYNHIYDLAVKAVPLGLIDYKANKPSAFLHWVESKGVDVPIALKQALFKQ
                     NQKVVQASIDGNEYLLKFNRNAHTAISKYLEWKNKNPLQRNMMSRVVEPILNTITSDT
                     REIEILKRVLKSQFSELDQ"
     gene            complement(12438..13619)
                     /locus_tag="U6309_000801"
     CDS             complement(12438..13619)
                     /locus_tag="U6309_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEN5504255.1"
                     /translation="MAEKKDITFRNAKPKDKAYKLADEKGLFLYVTPEGKKYWRFKYR
                     FNGKEKLLALGVYPDVTLSKARDKRNDARNLLNDEIDPGEHRKVMKKVDQDRSANSFE
                     VVAREWFVKYSANWVKSHADKIIQRFERDIFPWVGNKPITEVTPPVLLSVIRRIEERG
                     ALETAHRALSNCGQVFRYAIATGRAERDSSQDLKGALPPVKTKHFAAQTDPKRFGELL
                     RTFDDYEGSLIVRCALRLAPLVFVRPGELRHAKWADIDFETAQWSYIVSKTDTPHIVP
                     LANQAVSILKDLHPLTGNGIFVFPSARSTERPMSDNAILAALRRLGITKEEATGHGFR
                     ATARTLLDEVLKVRPDIIEHQLAHAVRDPNGRAYNRTKHLPERKNMMQEWADYLDELK
                     R"
     gene            complement(13785..14190)
                     /gene="ssrA"
                     /locus_tag="U6309_000802"
     tmRNA           complement(13785..14190)
                     /gene="ssrA"
                     /locus_tag="U6309_000802"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(14341..16356)
                     /locus_tag="U6309_000803"
     CDS             complement(14341..16356)
                     /locus_tag="U6309_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEN5504256.1"
                     /translation="MSTTVGLPQFSHIKIEHFKSHLDALLKNHLEEIDRLLKENHHYT
                     WDNLIYPLDSLADELERFWSPFAHMHAVMDSEAIRESYEACLPLLSAYDAAIGHNQDL
                     YEAIKSIDQHSLNPAQKKIIADSIQDFELSGVALSKANKKRFEAIQSRLAELSSKFEN
                     NVLDATHAYTIHITEAERVAGLPEHALNTAKELAHEKGLDGFVLTLEYPCFQAVITHA
                     EDRALREEMYRAYITRASDQGPNAGTFDNTPLIDEILSLRYEKAELLGFNNFAELSLA
                     TKMAASTNQVTEFIYDLISRTRDKGKAEFRQLEVFAQDKWNLSPVNPWDVAYLSEKRR
                     QDLYSLSQEELRPYFPQPKVMQGLFAIVKKLFGMGIEEIEGVDVWHKDVQCYCIVDES
                     NQTRGYIYTDLFARPHKRNGAWMDSMQSRRKLEDGTVQLPIATLTCNFAKPSANRPAM
                     LSHDEVVTLFHEFGHCLHHVLTQVDYLGGSGINGVEWDAVELPSQFFENWCWDKHALS
                     LLTSHVDTGETLPSALYERLIAAKNFQSAMAMLRQMEFALFDFRIHQEYQTGKASYVA
                     NILADVRSKTSVVPIVPYNRFQHSFSHIFGGGYAAGYYSYMWAEVLSSDAFARFEEEG
                     IFNPKTGHDFLKSILEAGGSRKAADAFIEFRGRPATIDALLRHNGIL"
     gene            complement(16456..17790)
                     /locus_tag="U6309_000804"
     CDS             complement(16456..17790)
                     /locus_tag="U6309_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504257.1"
                     /translation="MTPIISHLLKIFPELNTQVKTDSLNWTFNTHLKQLGPDFYSKVA
                     RLHPILNMEYSVLCQRLRYNLSAPDYSSPEQIKEQLIDALKLAELLEYTYQHYLVVPR
                     EVVRLRRHKAIYRELLTELSGYSFAMDNSEPESLKTSLSLTQAIREKTAQSNWYRIFI
                     SRSKRVINLLDNLDTGSKAFRDFVVLLDKYTNPFLAYLGWCFFAPRLFTNLFLILKHT
                     IPGEWMGEKEKSLDWSDRFYAHLQRRWFELANDSVWFTVGILNCFVLVGALAPLSVYL
                     SLFAFVFDVANTSLRAYIEIGRLQQLQKEYGELFDQEENEDQKKIIKEYQDSISYRIK
                     FEILRSFLSVGGAVAVVIAMALSVPALGIINPVIPLIGAVLLLALWAVSLYLNNKLDE
                     YRPVDNIEKPAPSVINKLSFFAPKHEKKESLHLSSKPEKDLEAEELIPTFTF"
     gene            18048..20054
                     /gene="tkt"
                     /locus_tag="U6309_000805"
     CDS             18048..20054
                     /gene="tkt"
                     /locus_tag="U6309_000805"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEN5504258.1"
                     /translation="MNSFTELANAVRILSIDAVNQAQSGHPGMPLGMADIATVLWKKF
                     LKFNPKNPHWFNRDRFVLSNGHGSMLLYSLLHLTRYNLDISELKNFRQLHSKTPGHPE
                     HGHTPGVETTTGPLGQGLANSVGMALAERVLASHFNHDHYNLVDHYTYTFAGDGCLME
                     GISHEACSLAGTLGLGKLIVFYDDNGISIDGKVESWFTDDTTSRFKAYNWQVIGPIDG
                     HDADQIEQAISAARENTTQPSLIICKTVIGLGSSVAGSEKAHGSPLSAQDINNVREFF
                     NWKHAPFEIPDSIYKQWDHREQGDKEEQQWLQLLHEYRLQYPSEHDEFLRRANGDLPD
                     DWQEISAGFLKQCLNNEKAIATRKASQQCIEFFAPILPEMFGGSADLTGSNNTDWSGS
                     KAITAHNFSGNYLYYGVREFAMAAIMNGLAVHGGFIPYGGTFLVFADYARNAIRLSAL
                     MKQRVIYVLTHDSIGLGEDGPTHQPIEHIAMLRMTPGMTVWRPADLMETAVAWQQSLE
                     HHNGPSALLLSRQNLPALAHGADAADLIKKGGYIIADCEGKPDAILIATGSEVQLAIT
                     AAEKAKARGLQVRVVSMPCAERFLAQSDAYKNQVLPGQVRTRIAIEAASSAYWHQFVG
                     FDGAVIGLDCFGVSAPATDAFNYLGITVEKIINTLDTLTKQTMV"
     gene            20136..21128
                     /gene="gap"
                     /locus_tag="U6309_000806"
     CDS             20136..21128
                     /gene="gap"
                     /locus_tag="U6309_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN5504259.1"
                     /translation="MTIRVAINGYGRIGRCILRSIFEYNRQNEFEVVAINDLSGIDVT
                     AHLTRYDSTHGPFRSDVSIEGNMLIVDGHGIQVIAERDPAKLPWKQLDIDIVFECTGF
                     FTSRDAAMGHINAGAKKVLISAPAKGVDTTVVYGVNHSVIKASDVIVSNASCTTNCLA
                     PVVKVLNDAIGIESGLVNTVHAYTKDQMLLDGSHKDLRRARSATQSIIPTKTGAAAAV
                     GLVLPELAGKLDGFAMRVPVLNVSVVDLTFQAKRTTSVEEVNDIMRKAKSRILQINED
                     PLVSCDFNHNPASAIFDTTETKVIGNLVKVVAWYDNEWGFSNRMIDTAHQMMNC"
     gene            21139..22329
                     /locus_tag="U6309_000807"
     CDS             21139..22329
                     /locus_tag="U6309_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEN5504260.1"
                     /translation="MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTI
                     KSALDSGAAVIVLSHLGRPEEGKYEKKFSLEPVADYLRKNLEYPVRFVKDYLSGVDVK
                     PGELVVCENVRFNPGEKANDEALAKKLANLCDVFVMDAFGTAHRAQASTYGVAQYAPV
                     AVAGPLLIRELEALNQVLKAPKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIA
                     NTFLKAQGFEIGISLYEPDLLDEARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFN
                     KSLSNVAEDDMILDIGPETIRDYVDLIHEANTIIWNGPVGVFEFPQFAYGTRAIAIAI
                     AESDAFSIAGGGDTLAAVDLYDLNQQISYISTGGGAFLECLEGKTLPAVAILQERAKH
                     VKTN"
     gene            22313..23737
                     /gene="pyk"
                     /locus_tag="U6309_000808"
     CDS             22313..23737
                     /gene="pyk"
                     /locus_tag="U6309_000808"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEN5504261.1"
                     /translation="MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSAL
                     QLIALVRKIADELNHPVAVMADLQGPKIRVGRFQNKSITLIDGQNFTLDCMAPDTLGD
                     INGVSVAYPNLANELSIGDHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNR
                     KGGGLAARTLTEKDRNDLRTAIEAEVDYISLSFVKDAEDIRQARALMKDYGAKITPII
                     AKIERMEALDHLTDIIREADAIMVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVIT
                     ATQMMESMISNPQPTRAEVSDVANAILDGTDAVMLSAETASGLFPVKVITMVNKICLS
                     AEKHASFFYHSDTETCHYQRADQAIAMATMHTANHFPIQAIITLTESGDTALWVSRHH
                     STVPIFAISANKRTIGRLSLVNNVFPIYIDFHQFNPEGLNQQILHELIKSGHLEKRGY
                     VLLTRGTQIGMPGGTNCMEIIPVV"
     gene            23828..29455
                     /locus_tag="U6309_000809"
     CDS             23828..29455
                     /locus_tag="U6309_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdhB"
                     /protein_id="HEN5504262.1"
                     /translation="MLLLSIEVIKMPDKKPSETLLSQQINEVRGLLLQALKTQLDDLV
                     AEKLIPQPSGVPFVDFEKDPPQIATLKKVINCLYHAEEAFKSWESIDTSTLLGKAKAA
                     PKLIRALTQIYKSLGLLDEASPEIRGVIADNYHLLEPLFTQAYAIVQESGWLSEFMEM
                     DVTDKASKVIFQGMDLLGPDIDKWHDTHPLVDAFSKISKLVETISTLQEKDMPGKEKE
                     QAVELVRALLDDLDNNPFISKLSISDFEDSKAIKDLLEWFKNIQDDGFDFSKKSIKQY
                     VSWANHYLPTLIAFADQLERQNYLKPGSLSTNLCSQIEQLGKQVNDILSEPGFGITER
                     VVTIDSLQRQREQKIHNAQVKSVQVIMATEKQQAATADFYRILEAYKGMNLSQITEPD
                     RRVLRQLYPQIQTALAHASLDLENQLTSVLNTTGPEKTPAKPKSWWELAKDGLGYVAS
                     YVVNYNVDKILGTRESVDRFLSSQIASEQFKILVAEKAREKLRTGSDKIKETALQYQA
                     EARVNAIKSTLRKQPAQMEATPSQFVAVKPSKLLNLRGTLTAIQEMQLSTTVNGTRTS
                     VDTLIHRHLPEKYQVCFSTLPYKINKNDPELVKQIKTVENNLFQLEKALKDFEKIDLS
                     YGIMIRLHYFIAIASAASKLKSSVKELSPEGQKALAPLLQQIMTLGLSFSDTNYKAND
                     LSALQQLKMEGQLRPKSPSVASPDTIGTLPEETVKRTAKKPKEKTTELLDYADKISNA
                     RALLLNKFKTTLSPALADGLNPQKKGVPFVHLDQDPPQIAAIKKIINSMYHAECALKT
                     WHGIDTSTTLGKVAAAHQGVAALSQVYKSFTLFTEDFSDIHNLIRENHDLIAPVIIEA
                     NKLVNQYGWARQFSELDIAQQLGSILGQGINTVQPKTDEITQTASLVKLFSEIPRLLN
                     NISSSLDQKSEKSIDELRISNKKIEAIGAVFELFFEENAYLMSMFKGFSAISGLIELN
                     KKIQREGTNLQEATIRQYQAWLKEGYPHLMMMLDEIETRHYLTPGLLSAPIALELDQI
                     NDKLNEIIETKPDFKLQKIPLSFYMGDKRIERLMTKKTEHCLALLQIEEQKKAVNTFF
                     GILKHYSGQPLSNIKPNELAQLRLAFVKIQSAMANSNLELSNDFVAALNQLETSPPEK
                     TKKIKVTVSQLLKVQPAVENYLNEHKKSCELKIAVIDKAIEHVKDKSFELKSGTSKEI
                     NIPQLRRTFLETESKKPVAGPGELKPTSVSSLNTVRGNLAYIQELKMSSTVGMMREQF
                     SALTRDNFSTRVQKYLIKPQDKPLHMINDNDPLMVRQIKEVENGLYHLETALKQFEQM
                     KKSDSLVSQTKALLEIANHALQLKMAVERFSPELKEHYGPLVRTVFSFSNKIQSINYN
                     KEDWADLQFILNRAHKELLKRKTPRDHRIEKAFFEAGIREVQIDRNESVGKKAAKLGV
                     KYAHLASPQLEKGRAYLSSRYKSAFGQQPKVIRSFTREQLANEEFMDAEINRLKKTLE
                     EKYGFNIATIKVLLALLEQIQRAGAQAAEVAGMVNTLVTYDFPKIKENAYRDLLTKLS
                     QEEDYLNLKPGTLINPAMAAVNQLFLSAALELDMPFSKKLSILDDTTYVNILIRQTES
                     DLQTLKKKLSEDPSNHEIAFEIEIKNDKLAFLRQQMVLLEDKNPNQTRNVLMDMQFEV
                     SLRNHLVYTTLKAPIAQEYEQIAREYYKKNKNRFLYADDCSKELLQGLKEFEKKHLPD
                     HLIVFEAYDRLRLFSAKLPPKNQDVKEYIENINRELQNRDIPIEQRALKVKSLPNDSV
                     FIEKLSSADKGTHFLRKFMQFITIITASITEAIKTGGSLIYIYRQKQMEQSIKNIEKS
                     ISFKEELHTIRDPDGPSPRKGEGKEGEIEFTKL"
BASE COUNT         9406 a         5922 c         5687 g         8590 t
ORIGIN      
        1 agtggaaaag gaaacaaata taaaatataa aatttcagct gttggtattg cgaacaagga
       61 aaaaccagag ctgtttctgc caaatccagg ttatgctaac agtattaata tttcttcagt
      121 agagaacgta acaattattg gaaaacataa taaagtaatt attcataaca aatataaaga
      181 gttaggcgaa aaaattgaag agcttattga tttggttcaa aacaatgcgg atattcccaa
      241 aaaagacaaa acagatattt tggctttaat aaaagcttta gaaagtctct ttatatctga
      301 aacaccagat attgaattgg ttaaaaaaac ggatgcagat ctggcttcaa aattaaaaaa
      361 atggtcggct actttagggg gtaatttact ggcaaacggt attaccagct taattacgtc
      421 ctactttagt atgtagctat tgaagagttg cttaaagatc gtttaggcac cgcaaataat
      481 aaatctaggt ttatggctaa tagtgcctaa agagcaatta ctttttgaat cgtaggctta
      541 tagtattcag catgccctga tttaattttc aggttcttag caagttctat agagacggcc
      601 gtatgtgtgt gaccacacaa aactaaaaat tggatatcag tatatttttt agcaaaggcc
      661 ataataacat caccagtagc ctttgaagca aaatacggca gccaattttc atcacttggt
      721 ttatccttgt gccaactgct ttcaggaaat ggtggaatat gagtggcaat aattactttt
      781 ttaatatcag tgccaattgc atcacacaat gtcgcgtgga ggactgcagc atctgtatca
      841 gccaattttt gcatttccct ttttaaggca gacttattca atagaaaagc ttggtataac
      901 tcagcaatca aacggctatc atttagattt actaagctat gatcaaaatc cccataacgt
      961 gcatctgccc atccatcatg cccaactaat acaataccgt cagctaaggt taaagtttta
     1021 ggctcaccaa gccaaatgag gtttttattc tgagcacaca aaaaacgaac cttttcacgt
     1081 acattattaa cgctactaaa gtagtagtcg tggttaccta atacaaaata aataagctta
     1141 tttgtgtagc tgctaaattc ttgaagaatc tcacaaacat cttttgcctc agctatatcg
     1201 ccagtaatta aaatcttatc tgcgccagtt tcaatgacct gttggtaaaa tttctttctt
     1261 gcttcaagct ttaagaagtt gaggtggata tcagttagcc aagcaacatt cattatttca
     1321 ccacatcaat aatggcattt atccgtgatt caacgatttt ctttcgacta tcagactcta
     1381 gtgaatagtt taaatgcccg ggtaatgctt ggagaaactg atcattactc attaatagtc
     1441 caaattcaga tgaaagatat tgctttaaat tgtctgatac gactaaaaca tcactagcga
     1501 tttctggccg gccatcaatt actgaaataa tatcttccaa atcatgactt aataaaaaat
     1561 cctgctttcc acgatcctta aacgcctcta acttggtagc tagaaaataa ggtgctgaaa
     1621 taacctttat atttatattg tcaaaaatac tatgtttgag agaattttct tgtgcttctt
     1681 tataccattt attagaaaac cccaggacac ttttatctat aggcatgaca tcaattaaaa
     1741 taccatcgca atcccaacga catataggat gatcaccaga aattagctcc ttaaatcctt
     1801 tttgccgtaa tttctcagca atagcataat aactcggtct agtgataacg ttgactatac
     1861 aatcaacatc cactgtaaaa cgtacatcag gagccgcctt atctgttatt aataaacctg
     1921 tgatacaacc cccgacaaaa gttacttcat cacataaatc cccaagccga tgagcgatta
     1981 aactgaggga ttcaatattt ttttgagctg aacttctatt catttgatac caactttttt
     2041 ggttttaata atgccaatat tttttgtgaa gcaatttgtc tttctcttac gcgcccagaa
     2101 cgaatagcat caattagggt caataagtca taaaatagcg gatctggatg ctttgctaca
     2161 gattcaggca ctgaagaata taatggtttt agggcaagac cacgctcgct cccttcgcca
     2221 tatggccata cagggatagg atcattacca gcaacaatct gatcacttaa ggatggagca
     2281 gcatagctag tgggaatacc acgggctagc tcgcctaatt tagcaggaaa aacatacttt
     2341 aaaccatgaa ggaaaaactc ttcacaagcc tgaataatgg gttgcggctt gcctggagga
     2401 tgatatggtg taattaaccc tactgcaact aatctcttga atgcactatt gacttgagaa
     2461 ggactcaaac acaattcaaa tgcaatacta ttttgagacc agcttttatc tgtttggtaa
     2521 gccaacagtt tggccagaac tacaatgtct tgtgatttaa tcattgagca tcatccttta
     2581 ttccatattc tagaatttag aataaagaat gcttttagtc aactatataa tgggcttatg
     2641 gacatattgt tggagtaatt ttaataaaga caaacgcttg acttcattat tcggctcgct
     2701 atacttttcc ccaggtcgtc gatatccccg gttatgagtg gccgaaaagc gatacttcaa
     2761 aataaaatct gtttgtagtt aatctttatt agctgctggc ggttttcctt aaacactcat
     2821 gaaaacgaaa ttaagaccag cttatttaat aaggaactac aaatgtccat tgcaaaactt
     2881 tgcgcggatc atcttcgtgc catttccaac aatcgtggtg ttaaactaaa atcaggacat
     2941 gctcatgagc ttgtcgcttc gttcttcggt tacaaatcca aagcagctat gttagcagat
     3001 acctcctctc caatagaccg acttaatcag gctcagtttc ttgttctaac tcccccacaa
     3061 tttattgaac aacgtcgaaa atgcctagaa aatttaccgt cagatttacc agataattca
     3121 atattaggag aagagctgtt tacttactta acattagaaa agtatttctc aggtaggcct
     3181 tttgcggcat ggaagcatct tgttgatatt cttgcaacag agtatctgtt aaaacatgca
     3241 agctcaatac tgtctataaa ttttggactt aatgaaatag cacacagtaa tttcgacaag
     3301 cctacagctg aatacattcc tgttccagat attacaggtg atggggtaac acttacggct
     3361 aaaatgcgct acgctgaaaa tgatattaat tctcaagcaa tagatataat agtaacaatc
     3421 aagttgcaac gcattgccgg tcatattggc tatgcaaatc ctaatatttc agttagtaca
     3481 ggaggttcta tatgatctcc tcccgcaaaa ttaaactgcc ctttggcttg aatgaaaata
     3541 acatgatcgc ccacattgct gacgttgaaa gcggtgaaaa atgcaactgt atttgtatcg
     3601 agtgtaaatc cccattgatt gcagtaaaag gccaaataaa agaacaccat tttcgacata
     3661 agagtgttac tgaatgtgaa gggggattag aaagcgccat ccatcttgca gctaaacaag
     3721 aaataatgaa aagaaagcaa ataacactac cccaatgcgt ttccaatgcc tcagcaacgg
     3781 actcaagagg aataaaacat acggaatata aaacgattgt tcaagacaag cagattatat
     3841 cgtttgatga tgtacaagaa gaaaaaatat tacatgaaat gaaagctgac atactggcaa
     3901 gtaaaggcaa cactcagctt attattgaaa ttttttatcg tcataaggtg gacgaagaaa
     3961 agatcaaaaa aataaagaag gcaaacatct cggcgataga aatcaactta tctgatctaa
     4021 caccagaaga tgtaaaaagc atggaaacat tctggtctcg cattaataat cctatttgtg
     4081 ttcaatggct atataatgtc aaagcccata attgtgtttc agagcttgcg aaccaattga
     4141 gggcaaagat taaaaagtta gaaatagaat atgcccaaga acaaatcagg aaacaaaaga
     4201 aagaagagaa agaaaagaac catctactac tagctctaga agacttaaaa gtacttcgca
     4261 gtaaacaaca cgcaatacaa cttaaccaag aggctgaaat gcatcctgcc tggaaacttc
     4321 acagccaata tctccagttg tcattcaata cattgcccag tttcctgaat ctagaggttc
     4381 ctgacggaga ttggattttc ggttgtgata ggcgtatctg gcaaacagct ttttatagct
     4441 ctttcattca gaaaaacggc aaacccttct gtattagaag agttgacgaa tggcttaata
     4501 ataaagctaa gtgcgaggtt cccttcacgg cccaaattgt aggaatatat ggcaggcgtt
     4561 accctgaact tgttccttct gatgtttcaa tcaatctgcc atcgacatgg agaactttgc
     4621 aaagttattt taaacatcta tgtgatttag gaatgctaga attttccccg agtgaagggg
     4681 gatggggaaa agactcttgg tttagaatta ttaacaacac accaccaggc tcagtgcaaa
     4741 tagctattga aaaaaacctg atgaaatgga atataaataa gattcaataa aaaataatag
     4801 gagaacaaag atggaaatag gatttttgaa tgcttactaa acactctata tctaaaccaa
     4861 taaatatcac agaatacact aggccaataa aaattttata tatagtgccg tataaagaaa
     4921 ctcgcaaaaa tcacttaatt ttagatgctg tattttatga ttcatatact agatggagtg
     4981 gcgctgctgt atttgttatt cctgcaaaca aaaatggttt tttgtatgag atgtatgatg
     5041 aatggtttga aaaccttgat cctgattttg tttgttccta tgttgaattg actcaaacat
     5101 tcattaaaaa aatatctaaa ttatgtaacc ctattttaat ttcagtagaa gacagtaaat
     5161 atacgtcaaa agattcattt cgacgttact taaaagattg gcagcgtgtt gcacttattc
     5221 aaccgctatc ttctttatca ctagtttccg cgacaaaaca gtattttgaa ggcagagagc
     5281 ttggtaaagc aaataaaata cttgttgaaa tacagcctaa cgaaagtaat cgttttattt
     5341 ccgataattt tggatgtaat ggccatcaat atagtactac ccaaacggtt cttggtttgt
     5401 ttaatgttgt ttgccttaat ccatacgcca aagacggata tgttagcgaa ggtactgaac
     5461 tagtaaaatc atacaaagat gtattatcaa gaataggaag tcgagagata aatacttttt
     5521 tagatctatc agtttcttat agtacagcaa ttccgaaggt aaatataaat aataatgagt
     5581 tagcttttaa tgtgtttctt ggagatacat gcattgaccg tatcaatttt tggaatagtc
     5641 gatgcttaac gcaagataga aaaagttcga taatacttag taaaaatcaa tactccgatg
     5701 atgatttttt gaaactattc ggagagtttc tgaattcata caatgagcgt tatgggcaca
     5761 ataaccaacg tgaagttgaa gtaagaagtc taagtataaa tttagatgaa agaaaagagt
     5821 tcgctaggaa gttaggcaac aatacacgat gcatggtatg gactaatgag aaaagctcca
     5881 gagaggtatc gataagtcgt gataacttcg gcaaattgta tacaagtgtt gatgtacagt
     5941 catcacacta tcaaacaact aaaaaagaaa gtaatggtta tgttgctaat gacccttatc
     6001 attttaaata tatttcacca cattatgatt ttctaaaaga tggtgattgg atggttgagt
     6061 tagaaataga aagagagaag gatctatcga gatatgataa tgtcacccat gtatggatgc
     6121 tgcccattag agaaaacagc tcaaagatat ttaccagtaa tcttagtaga gttacaaaaa
     6181 atcatatcat tgcgcttatt ccaagtcgta aggataggca taacttcttt aagcaagata
     6241 ttatgagatc gggtgcttca tatcaattat catttccaag tgatgagtca tattttagct
     6301 gtatagtgac aaactacaac actgattatt ttcctgaaga tccaagagcg aagcttcgac
     6361 ataaatcata cgagcgtatt ggagtgtctt caaaaggcaa atatatgcgg ggaattataa
     6421 gaaaaatagg ttctttatat aaggcatact attcgctaac taggaaatta tggagggatt
     6481 gtattcgcaa agaagccgta aaggaagatc ttaagtttgc gagttttaac ggtttacttg
     6541 aaacggctct gggagatagt aaatttagga aaaaatatca gaataaatat aatgcatcta
     6601 atgatcagca agtcaaatct gatataaaaa agaatttttt cgcttgctct aatttgttat
     6661 tgcaacagaa agttatatat aaagttcacc catgggtatg tgactactgc ggcactcacc
     6721 acgtgtgttc tgtagataat ctgaagtttg aaaatacgtg cacagtttgt ggagagatct
     6781 accatgtgcc atattctatt gaatggcgct ttcaaattga cgcgatgatt attaatgctc
     6841 tacatagaaa taatggttta actgtattgt ggatgatagg ttatatttca actcaagatt
     6901 gccgtgtgag tagtttttat tacatgccag aggttgatct ttttaattct tataaagaca
     6961 aagagagatc ggcagaaatt gatcttatat gtgttgtaga tggattgctg tacattggtg
     7021 aagcaaagca aacagcagca ttatttttaa agaaaaagaa agataagaaa gatgaggaag
     7081 ctaaattcat cgagattata aaaaaaatca aaccggatgt ggcgttatta gcatttgaaa
     7141 gatattccga tttagaagaa gacacggata aagtcaggac aaaacttatt gcctcaatag
     7201 agagaatgaa aaaaatatgt ggagatgttg atataagata tattgtggca gaggaggtag
     7261 atgaaggctt caatgaattt ttatgccaag attttgaaat atcctaaatc aaagtatagg
     7321 tttagatttt tattgagata gaaaactgac acaattaata attatcaatc attcctaaaa
     7381 atgacttttt ctttgatatt tcaagttgat taaaccattt aataatagca gctagatcat
     7441 cttcttctgc ataaaaataa gcggtaggca ctgataatgc ttgagctatt ctttttagag
     7501 tcgagtaatc cggggcatgt ctacctagtt cataatgatt catgcgcgcg ctggcggaga
     7561 actcatccat accagcagca atccctagct tcttttgaga tatattcgca gccaatctgg
     7621 cttctttaag tctttttggg acaggggatt ccatctaaat caatttttgt aaagaacata
     7681 cttagataat ctaagtattt ctcacaccaa gatacttagg aatcctatac atttgtattc
     7741 ggttgtgtat aatgtctcca aattggccag ataaccggtc agaaaaatta tctgggtaaa
     7801 tgtaggaaat aataagaaca ataaggagcc cacattatta gtctatagag aggttccgtt
     7861 atgattgttg ttgcaagctt attattgctt ggatttttgg tatacgatgc aatacgttca
     7921 tcaaaaaata aaaattcatt agatttagaa aagattttca aaagttctag caatctttgt
     7981 atgcaattca ttgaaatgca gattttccat gcttgcacat atcatgctta tgctcttaaa
     8041 caagaatggg atggagatgt tatttggtca attgtaagat ccattactac agacatatat
     8101 aacgacgggt tattcacgga atttttaccg ttatactatt ttgggataac ttttgcagcg
     8161 acagagcggg ttaaccttaa caaatcgtca gcctcatata tgcaactaca gcttgcgcta
     8221 atgtcaaaag tctataatgc agagcaagca gccataaata tgcactctat aatcacttca
     8281 caagcccaag cccccattgg aggaaagcca actcatacat catcaaccat tgttgttggc
     8341 catgcccagt tcgattcttt tataaactat tgctctacta aaatgaatca tactaatgta
     8401 gagcttttat ccttaccagc taccctcttg cattattgta ttgaaaacga tttaaatgct
     8461 aaagaagcct ttgcgtttta tgattttctg ctcgaaaacc ataaagatta tctgatcaat
     8521 aaaataaact tttatctcta ataaatcaga taaaaccacc tcattctagc tcatcaaaaa
     8581 agcatccagt cttttgatgt gactcaattt catcaaggcg cttggcaagc tcttcctgtg
     8641 ctaggaattc cttttgtgta ttaataagat gcataaattg attgccatct tgcactgaaa
     8701 gagctccttt tgcagtagcg tctataattt tttttccaat tataggtagg gtcgtgctac
     8761 gttcaatatc taatccttgt agatcaatct gtatttgttg gccggttact ttaggtatca
     8821 ggcgctctat acatagtcgt aatgcattaa catcgccatc catcgctagt tcaacagtct
     8881 tattaatcaa cccttctgca taaggttcaa gtaacgttgc aagcctagtt cgcttgttga
     8941 gtgtacctgg ctttttaccg ccagggttgc cggactgtcc ttttttaaat ttagtcattt
     9001 gtgttcctta tctgcatttt ttctgctttt tacaggaaga ataccctttt atttcctaat
     9061 tcgtatggat ttaaaaagtt ttattgcaag tcttgaatgt cttaatgtct tcaatctata
     9121 acataatgag aagactttaa gacatccaag acttgggatg cttcatgttt tgatagattt
     9181 aatctcccaa cttacataac ttccttgctt ccctaagctt ctacatgaga tccccatttg
     9241 tcttaatgct ggtgcagctc tacgtaaacc atcacctaat cccttgggcg ttttaggcca
     9301 gttatcagga ccaggtggac gatacttttc taggcgctgt aacaagtgtt tgagtggcat
     9361 ggttattggc aaagcgagat tatcatcagc catttcaatt attgctgatg caactggact
     9421 ggcatcgatt gttcttgcga tagcttctgt ccgatttaaa ttaaaagcct ctagaaaatt
     9481 attactcgag catcctatta cttctgaaat tgccatgccc aaattagcaa actcaattag
     9541 ccttggacaa ttcgattttg gtaaattgat agatggtaat atgctaaggg cctcggcaaa
     9601 gatatctaat agtgcgccta acatctgcga atgattatca gagtaaatat tcctcaaccg
     9661 attgctttca gctctttcct ccacaagagg agtctctatc gatattgttc tatcaatcaa
     9721 atcctgagat gtaattgatg cacatatacc attcaataca ataggccgct tcacttttat
     9781 ctgtgtttca tccgcattag aatataactt tcttttagca taacctccac ctgtagaaag
     9841 aacacaaaac gcatcctgaa ttttctgagg aagataacta atattctcat agctaacgac
     9901 ccagttcata ccaccagcaa tataaacatc ttcatctgtc ttaggtgagg ctcgaaggtt
     9961 gcatgagtta ggatcaataa gttgcctaag aaactcttgt gttgtgcttt tagcgcttcc
    10021 ttgttctccg actaacttta gcaatggaaa aggtgtttcg ggtcgcaagc aatcaataag
    10081 ccaggcaagc accaggagct gcatatcctc tggaatattt accacttgcc atagggcatt
    10141 tatacttccc cctttaagtg gtattggtaa tgggctcatt gcatctggcc gcacaaacct
    10201 tattgggtgg tcatcaacaa cgtgccaaga tcctggtgag atacaaatag cacgattttt
    10261 acctgtttca ctcaaatcta agtagtacgc gtcattgagt attgccaccc ttatgaaaac
    10321 gtcatgtcgc tcacctttaa attgagctaa accttttagt gtttcaattg cctctctaat
    10381 tgagtgctct cttggcgttt tatcatgtac ctcataataa ttggaaacaa gccagctttt
    10441 aaattgccgc ccatcaagct tacgtgtctc attagttagt aagtcataag cataagtttc
    10501 cttgtttgaa tcaaaaaaca attctgcacg ctcaataaca aactctacta tatatgcggc
    10561 ctcactttga ggcttgtcat tatctttaaa acctttatca ttgctaatgg aacttttctt
    10621 atcacttttt atcaaagttg aatttaattt tttataggga aggctctcga ttacttcttt
    10681 tgtggggttt gggtgctctc tcatccaatc caccacatca cctttcggcg gtagctttaa
    10741 ctgttcgata tcaattagct ttatgttaac acccaagccc tgaaaaactt taatagcatc
    10801 atttgcataa cgcttccctg cttcatcatt atcaggccat acgattaatt ctctatcctt
    10861 tattggttca aagtcagctt tgctaataga gttagcaccc ccacttgtgg tagcaagaca
    10921 acccaattga ttaaagatat ctacacacca ctctccttca caaaaatata ttggttcatt
    10981 tcttctgttt attatttggg gcaaattata taacggcttc tttttattga acttgggctc
    11041 accgaacaca taagcattac cctcatacct gagcggacgg atgatcttct catttgtatc
    11101 gggatttttt aaccttactc tatggtagaa ttcctctcca ttagcatcgg tataagtatg
    11161 aattgcttct aacttaaaac cttcatttaa ctgcttcgaa aataatcttg gtaccacatc
    11221 ttcaattctt tcctttgaaa tagacattat atttcttcct cccatgcctc aagttcaata
    11281 gctgtatctt taaaaccaag gccatatttt ttacgatgaa aagcgagaac atctccacct
    11341 ttggctccac aagccataca acaaaaacaa ccttcatcaa gacgtactcg caagcttggt
    11401 tttgtatctt catgaaaagg gcaaagagct gtcttccact caagtcctcc agataaattt
    11461 aagccttgcc ctttatagta ttttgatggg gagggtaatc gttcacgatt aaagttttgc
    11521 tttttcatta gaagatactc ccttagcaat ttgacttctt gaagcttcta attgcttaac
    11581 aagtacacga acttcatcat ttggaagacc tgcaatacga gctgcattta aggctgtgat
    11641 ttcattttct ggccaagcaa ctgagcgtcc acccaatgaa acaggcttag tccatagtcc
    11701 ttgggagatc cgtaaataga gagtactacg cgaaagaccc gatgcagatt gtacttttgg
    11761 tcttcttaat aaagagctaa ccatattatg cctccttagg cgttgttaaa tatagttaga
    11821 gtttgataga tctatttttc tgtcacatca tctgtcagct gacagtggtt gttgaaaaac
    11881 aactcacttt attattgatc aagttcactg aattggcttt taagcactct ttttagaatt
    11941 tcaatttctc gggtatcact tgtaatagta ttaagaattg gctctacaac tcgactcatc
    12001 atgtttctct gaagaggatt tttgtttttc cattctaaat atttagatat agctgtatgg
    12061 gcatttcgat taaattttaa taaatactca ttaccatcta tacttgcttg cactaccttt
    12121 tggttctgtt taaatagtgc ttgcttaagt gctattggca catcaacccc tttagactca
    12181 acccaatgaa gaaaagcact tggcttattt gctttataat caattaaacc aagaggcaca
    12241 gcctttactg ctagatcata tatatgattg tatctctcct ttgcattctc atttcctcga
    12301 aactcatgtc ctacaactga cgaagctcta gggttgaaac ccaaaacaag aagtattccc
    12361 tcccttaaat tccatgaatc atattggatc cacacactat aaatacaacc atcctcacct
    12421 actgcattca taagttctca cctcttcaat tcatctaaat aatcagccca ctcttgcatc
    12481 atatttttac gctcaggtaa gtgcttcgta cggttgtagg cacgtccatt gggatctcga
    12541 acagcatggg cgagttgatg ttcgataatg tctggtcgaa cttttaatac ttcatctaat
    12601 aaggtgcgtg ctgtggctcg aaatccgtga cctgtggcct cttccttggt gatacctagg
    12661 cggcgtagtg ccgcaaggat ggcattgtca ctcatagggc gttctgtgct tctagctgag
    12721 ggaaaaacaa aaatgccatt acccgtcaat ggatgaaggt cttttaagat agacactgct
    12781 tgattagcta aaggtacaat gtggggagta tcagttttag aaacaatata gctccactga
    12841 gcagtttcaa aatcaatatc agcccatttt gcatggcgta attcacctgg tcttacaaat
    12901 actaaaggag ctagccgcaa agcacagcga acgattaaag atccttcata atcgtcaaaa
    12961 gttcttaata actcaccaaa acgcttagga tctgtttgtg ctgcaaaatg tttggttttt
    13021 accgggggta atgcgccttt taaatcttgg gatgaatctc tttcggctcg gcctgttgca
    13081 atggcataac ggaatacttg accacaattg cttagtgccc tgtgggctgt ttctaatgct
    13141 ccacgctctt caattcggcg aattactgac aacaatactg gaggtgtaac ttctgttata
    13201 ggtttattgc caacccaagg aaaaatatca cgctcaaagc gttgtatgat tttatcagca
    13261 tgactcttaa cccaatttgc tgaatatttt acaaaccatt ctctagcaac tacctcaaag
    13321 ctgttagccg agcggtcttg gtcgactttt ttcattacct tacgatgctc gccaggatca
    13381 atctcgtcat taagtaaatt tcgagcatca tttcgtttat cgcgagcttt agaaagagtg
    13441 acatcgggat atacacccaa tgccaataat ttttctttgc cattaaaccg atacttaaaa
    13501 cgccaatatt ttttaccttc cggtgttaca tagaggaata agcctttctc atcagcaagc
    13561 ttgtatgctt tgtctttagg ttttgcatta cgaaaagtta tatctttctt ttcagccact
    13621 ttgggggtat ctcctttttg acttatcgat ataccccaat aaataccccc actaagatgt
    13681 ggatgtaggg ggtatctatt ggacaatagt ggacgtatga taggctttaa agccagtatt
    13741 tacaagggat tatggatgtt attggagtgt cttggatggt aaattggtgg aggcggcggg
    13801 aatcgaaccc gcgtccgcaa gtcctctgcc tttagctcta catgcatagc cttgtctttt
    13861 gaatttaact gtggctcctc cgacgggcag gatgatacac agcgattccc tgagtttcgc
    13921 gccataaccc gggatagatt aggacgctag cttatctctt atgaccgttg attcgatacc
    13981 gataagcggg ctcggtcgaa cgggatactg ggtttattgg ccagtacaca gtgatggttt
    14041 aactaaagtg cttattaggc agcgatagct tccccaccag caaagttttc atcgtttgca
    14101 tttatattta attgagtctt atttacgaga gatctccttc tcggcatgca cttccggttt
    14161 tgcaacccac gtcgaagcca ggtcgccccc cattctgtac actttaatta taacataaaa
    14221 tgccggcatg taagcaaaat cccgccagca taccgaatat attttcagga tctataaaaa
    14281 atcccaaggt caaggcaaaa aatgttttac cgagagcgct ggggggcgca agtcctgttt
    14341 ttataaaatc ccgttatgac gcagcaaagc atcaatcgtc gcgggtcttc ctcggaattc
    14401 aataaaagca tcggctgctt ttcttgagcc gcctgcctcc aaaatggatt tcaaaaagtc
    14461 atgtccggtt ttggggttga aaataccttc ttcctcaaag cgcgcaaagg catcgctgga
    14521 tagtacttct gcccacatat aactgtaata accagcagcg tagccacccc caaaaatatg
    14581 cgagaaacta tgttgaaagc gattataagg aacaatgggc acaacactgg ttttagaacg
    14641 tacatccgcc agaatatttg cgacatagga cgctttaccc gtttgatatt cctggtgaat
    14701 gcgaaaatca aacaaggcaa attccatttg tcttaacatg gccatggcgg attgaaaatt
    14761 tttagcagca atcaagcgct cgtacaatgc ggaaggcaag gtttcccctg tatccacgtg
    14821 cgaagtcagt aaagacaatg cgtgcttatc ccagcaccag ttttcgaaaa attggctggg
    14881 taattccacc gcatcccatt ccacgccatt aatccctgag ccgccaaggt aatcgacttg
    14941 agtcaaaacg tgatgcagac aatgaccaaa ctcgtgaaat aaagtcacca cttcatcgtg
    15001 cgataacatg gccggtctgt ttgcagaagg cttggcaaaa ttgcaagtca aggtcgcaat
    15061 gggtaattga actgtgccat cctccagttt tctgcggctt tgcatggagt ccatccaggc
    15121 accattgcgc ttgtgcggtc tggcaaacaa atccgtgtaa atgtacccgc gtgtttgatt
    15181 ggattcatcg actatgcaat agcattgaac atccttatgc caaacatcca cgccctcaat
    15241 ctcctcgata cccataccaa agagcttttt aacaatcgcg aacaagcctt gcatcacttt
    15301 aggctgcggg aaataaggcc gtaattcctc ttgagataag gaatacaaat cctgtcttct
    15361 tttttcagac agataagcaa cgtcccatgg gtttaccgga ctcagattcc atttatcctg
    15421 tgcaaacact tcaagctgcc taaattcagc tttacccttg tcacgagttc tgctgatcaa
    15481 atcatagata aattcagtga cttgattggt cgaggcagcc atcttggtag ccagggataa
    15541 ctcggcaaaa ttattaaacc ctaaaagctc ggctttttca tagcgcaggg acagaatttc
    15601 atcaatcagc ggagtattat caaaggttcc tgcattaggg ccttgatctg aagctctggt
    15661 gatgtaagct cgatacattt cttcacgtaa agccctgtct tcagcatgag taataacagc
    15721 ctgaaaacat gggtattcga gggttaaaac aaaaccatcg agccctttct catgcgccaa
    15781 ctcttttgcc gtatttaatg catgctcagg taatcctgcc acacgttctg cttcggtaat
    15841 atggatagtg taggcatggg ttgcgtccag cacattgttt tcgaatttgc tggataattc
    15901 ggccagtctt gattgaatgg cttcaaagcg ctttttattc gccttggata aagcaactcc
    15961 cgatagctca aaatcctgta tgctgtctgc aatgattttt ttctgggcag ggttaaggga
    16021 atgctgatct atcgatttaa tcgcctcata caagtcttga ttatgcccaa tcgcagcatc
    16081 ataggcagac agcaatggca gacacgcttc ataggattca cgtattgcct cagaatccat
    16141 caccgcatgc atatgtgcaa aaggagacca aaaacgctcc agttcatcgg cgaggctatc
    16201 taaaggataa attaaattat cccaggtgta gtggtgattt tccttaagca acctgtcaat
    16261 ttcctcaaga tggtttttta ataaagcatc aagatgagat ttgaaatgtt caatttttat
    16321 atggctaaat tgcggcaatc caactgtggt cgacatgtta tacctttcca tcatgtaatt
    16381 ttttgaaagc ataacattca attgaattag tctctatact ttgccggcgc tgaaatggca
    16441 aagtacagta atgaattaaa aggtgaacgt tggaatcaat tcctctgcct caagatcctt
    16501 ttctggcttg gaagacaagt gcagactctc ttttttttca tgtttgggtg caaaaaaact
    16561 taatttattg ataacggaag gagcaggttt ttcaatgtta tcgacaggtc tgtattcatc
    16621 taacttgttg ttcaagtata atgaaacggc ccatagcgct agcaaaagca ctgccccaat
    16681 caaggggatg acaggattta taatacccaa agcaggaact gataaagcca ttgcaatcac
    16741 aacagcgact gccccgccaa ccgataaaaa ggagcgcaaa atctcaaact taatgcgata
    16801 actgatcgag tcttgatact ctttaattat ctttttctgg tcttcatttt cttcctggtc
    16861 gaagagttcg ccatattcct tttgcaactg ctgtaatcga ccaatctcta tgtaagcacg
    16921 aagacttgtg ttggcgacat caaagacaaa cgcaaacaaa gacaaataaa cagagagagg
    16981 cgccaaagcg cccaccagca cgaagcaatt gagaatcccg actgtaaacc aaacgctgtc
    17041 attggccagc tcaaaccacc gccgttgcag gtgggcataa aatcgatcgg accaatccag
    17101 tgatttttct ttctcgccca tccattcccc tggaatggtg tgctttaaaa ttaaaaagag
    17161 attggtaaat aatctggggg caaaaaaaca ccagcccaaa taagccaaaa atggattagt
    17221 gtatttatca agcagcacga caaaatcacg aaatgccttt gaaccagtgt ctaaattatc
    17281 caaaagatta atgactcgtt tacttctgct gataaaaatt ctataccaat tggattgagc
    17341 agttttctca cgaatggcct gcgtcaacga taagcttgtc ttgagagatt ccggctccga
    17401 attatccatg gcaaaagaat acccggacaa ttccgtcaat aattccctgt atatggcttt
    17461 atggcgacgt aaacgcacta cttcgcgtgg aacaaccagg tagtgttgat aggtatattc
    17521 caaaagttca gcgagcttga gtgcatcaat cagctgttcc ttgatttgct cgggagaact
    17581 gtaatcaggc gcggacaggt tataccttaa gcgctgacac aatacgctgt attccatatt
    17641 gagaatggga tgaagacgag ctactttcga ataaaaatca gggcccaatt gcttcaggtg
    17701 cgtgttaaaa gtccaattca aagaatcagt tttaacttgc gtattcagct ctggaaatat
    17761 ttttaataaa tgactaataa tcggtgtcat gattgtttga aataaataat ccgcatatca
    17821 ttgtgtttct aatccagctt tgcaaaaatt tttgctcctg tcaccttatc tgggctgcaa
    17881 aaattaatca aactccatca ttatgtcaaa aattaggaaa aatttaaact taaatttaca
    17941 ttttgctcta atattgtcat tattttcaac taaaaatgaa tggtcttctt gccgatttat
    18001 gccttctgag cgacaatagt gcctcagtta aatgcaaaag agtaggcatg aacagtttta
    18061 cagaattagc taatgcagta cgcatattaa gcatcgatgc agtaaaccag gctcaatcag
    18121 gccaccctgg catgcccttg ggtatggctg atattgccac cgttttatgg aaaaaatttc
    18181 taaaattcaa ccccaaaaac ccgcactggt ttaacagaga ccgttttgta ttatctaatg
    18241 gccatggttc catgcttctc tattctttac tgcatttgac cagatacaat ctggatatta
    18301 gcgagcttaa aaacttccgc caattgcact caaaaacacc agggcatccg gaacatgggc
    18361 acactcccgg tgtcgaaacc acaacagggc cattaggtca aggtttggct aattcagtcg
    18421 ggatggcatt ggcagagcgt gtactcgcca gtcacttcaa ccacgatcac tataacctcg
    18481 ttgatcatta tacttatact tttgccggcg atggatgcct gatggaaggc atttctcatg
    18541 aagcatgttc tctggctggc actcttggtt tgggaaaact cattgtattt tatgacgaca
    18601 atgggatatc tattgatggt aaggtagaat cctggtttac ggacgatacc acgtctcgct
    18661 ttaaagccta caactggcaa gtcatagggc ccatcgatgg ccacgacgca gatcaaattg
    18721 agcaagccat atcagcagcg cgtgagaaca cgactcaacc ttcattaatt atctgtaaaa
    18781 ccgtcatagg actgggttcc tctgtagccg gtagcgaaaa agcgcacggc tctccattga
    18841 gtgcccaaga cataaataat gtccgtgaat tttttaattg gaaacatgct cccttcgaaa
    18901 ttccagattc gatatacaag caatgggatc atagagagca aggagacaaa gaagagcaac
    18961 aatggctgca gctcctgcat gaataccggc tacaatatcc atcggaacac gatgagtttt
    19021 tgcgtcgtgc caatggcgac ctgcccgatg attggcaaga aatatcggct ggattcctaa
    19081 aacaatgcct gaacaatgag aaagccatag caacgcgtaa agcatcgcag caatgcatag
    19141 agtttttcgc gccaatatta ccggaaatgt ttggcggctc agcggatttg actggttcca
    19201 ataataccga ctggtcaggc agcaaagcca tcacggccca taatttttcc ggaaattacc
    19261 tttattatgg agtgagggaa tttgccatgg cagccatcat gaatggactt gctgttcatg
    19321 gaggattcat tccctacggc ggaacattcc ttgttttcgc agattatgca agaaatgcca
    19381 tccgtttaag cgctttaatg aaacaaagag tgatatacgt attgactcat gattctatcg
    19441 gtttagggga agatggcccg acacaccaac ctatagaaca tatcgctatg ttaagaatga
    19501 cccctggcat gacggtctgg cgccctgctg atttgatgga aaccgctgtt gcatggcaac
    19561 aatcgttaga acatcataac ggaccgtctg cattgttact ctcaagacaa aatctgcccg
    19621 ctttagccca tggcgccgat gcggctgact tgattaagaa aggcggatat attattgcag
    19681 attgtgaagg gaagcctgat gctatcctga tagccactgg ttcagaagtt caactggcta
    19741 taaccgcagc agaaaaggca aaagcaagag gtctccaggt cagggtggta tccatgccct
    19801 gtgctgagcg attccttgcc cagagtgatg cttacaaaaa ccaggtatta cctggtcagg
    19861 ttcgcacacg tattgcgata gaagcagcta gcagtgccta ttggcaccaa tttgttggct
    19921 ttgacggcgc tgtcattggg ctggattgtt ttggggtatc agctcctgct acagatgctt
    19981 ttaattacct tggaattaca gtagaaaaaa ttatcaatac tctagataca ttaaccaaac
    20041 aaacaatggt gtaacccata ccccttctga agtttcaaga aggcctgtat tcagtataac
    20101 tgagtatgga ggttacccta actcggagaa gtagtatgac aatacgagtc gcgattaatg
    20161 gctatggccg cattggccgt tgcattttaa gatcgatttt tgaatacaac aggcaaaatg
    20221 agtttgaagt cgttgccatt aacgatttat ctggcattga tgtgaccgct cacctgactc
    20281 gctacgattc aactcacggt ccttttcgct cggatgttag catagaagga aacatgctga
    20341 ttgttgatgg gcatggcatt caagtgattg ctgagcgtga tccggccaaa ctgccatgga
    20401 aacaattgga cattgatatc gtctttgaat gcactggttt ctttaccagc agagatgcgg
    20461 caatgggtca tattaatgct ggcgccaaaa aagtattaat atctgctccg gccaaaggcg
    20521 ttgatacgac agtggtctat ggagttaatc actccgtcat caaagcgtct gatgtcattg
    20581 tatctaacgc ttcctgtaca acaaactgcc tggcgccagt agttaaagtg ttaaatgatg
    20641 ctataggaat tgaatccggc ttagtcaaca ctgtccatgc ttacacaaaa gatcaaatgc
    20701 ttttggatgg aagccataaa gatttacgac gcgcccgctc tgcaacgcag tccatcattc
    20761 caaccaaaac cggcgctgcc gcagccgttg gattggtttt acctgaactg gcagggaaat
    20821 tggacggatt tgcaatgcga gtaccggtat taaacgtatc cgttgttgat ttaactttcc
    20881 aggcaaagcg cacaacttct gtggaagaag tcaatgacat catgcgcaag gctaaaagcc
    20941 gaattttaca aattaatgaa gatcctttag tttcttgtga cttcaatcac aaccccgctt
    21001 ctgctatttt cgacacaaca gaaaccaaag tgataggcaa tttggtcaaa gtagtagcct
    21061 ggtatgacaa tgaatggggt ttctctaatc gcatgattga taccgcacac caaatgatga
    21121 actgctaaag gataaccaat gaacctgatt aaaatgagcg acattgatct gtctggcaaa
    21181 agagttctga ttcgtgagga tttaaatgtc ccgatcaaag acggcatgat taccagtgat
    21241 caaagattgc aagcggccct gcctacaatc aaatccgctt tggacagtgg cgcagcagta
    21301 atcgtattgt cccatcttgg gcgtccggaa gaaggcaaat acgaaaaaaa attctctttg
    21361 gaacctgttg ctgattattt aaggaaaaac ctggaatatc ccgtccgctt cgtcaaagat
    21421 tatctgagtg gtgtggatgt aaaacctggg gaattggtcg tttgcgaaaa tgtccgattt
    21481 aatcccggtg aaaaagccaa tgacgaagct ttggcaaaaa aactggcaaa cctttgtgat
    21541 gtctttgtca tggatgcttt tggcacggca cacagggcac aagcttctac ctatggtgtc
    21601 gctcaatatg ctccagttgc cgttgctggt cctctgctaa tcagggaact tgaagcgtta
    21661 aaccaggtgc tcaaagcccc caaaaaacct atcgtcgcca ttgttggagg agccaaagtc
    21721 tcatccaaac tcagtttatt gaaacaatta gtgggtatgg tcgatgttct catccctggc
    21781 ggaggtatcg ccaatacttt tctgaaagcc cagggatttg aaattggtat ttccctttac
    21841 gaacctgatt tattggatga ggctcgccat atccttattc tggccaaaga aaaaggttgc
    21901 caaattcctc tacccaccga cgttgtagta ggcaaaacat ttagtgaaac ctgccctgct
    21961 ttcaacaaat ctctgtctaa tgtggccgag gacgatatga ttcttgatat tggaccagaa
    22021 accataagag attatgtgga tctcatccat gaagccaata ccatcatctg gaatggtccg
    22081 gttggcgtat tcgaattccc gcagtttgct tatggaacaa gagccattgc tatagcgatt
    22141 gccgagagtg atgccttttc tattgctggc ggtggtgata ccttggcagc tgttgacctt
    22201 tatgatttga atcaacaaat ttcttatatt tccacaggag gtggggcatt tctggaatgc
    22261 ctggaaggaa aaaccttgcc tgctgttgct attttgcagg agcgcgcaaa acatgttaag
    22321 acgaactaag attgttgcca ctttaggccc agccagtaag gaacctgaaa ttctgcgatc
    22381 catgctggct gctggagtca acgtagtacg aattaacttt tctcatgccg attcctctgc
    22441 cctgcaatta attgcactgg ttcgtaaaat cgcagatgaa ttgaaccacc cggttgcagt
    22501 catggctgac ttacaagggc ctaaaataag agtcggccgt tttcaaaaca aatccatcac
    22561 tttaattgat ggacaaaatt ttacattgga ttgtatggcc ccagatactc tgggagacat
    22621 aaatggcgta tcggtcgctt atcctaactt ggccaatgaa ctcagcattg gtgatcatct
    22681 gctgattaac gatgggttaa ttgaattgga agtcatagag atttccgggt caaaaattca
    22741 ttgcaaggta gtggaaggtg gagtactcac tgatttgaaa ggtttgaatc gaaaaggtgg
    22801 cggcttagca gccagaactt taacagaaaa agatagaaat gatttacgta cagcaattga
    22861 agcagaagtg gactacatca gtttatcttt tgtaaaagac gcagaagata tccgacaagc
    22921 aagagcacta atgaaagact atggggccaa aataacccct attatcgcta aaattgagcg
    22981 catggaagcc ttggatcact taactgatat tattcgcgaa gcagatgcca tcatggtggc
    23041 cagaggagat ttaggcgttg aagtgggtgc tgctgaagtt cccgctattc aaaaacacat
    23101 catagaacaa acgcgacttt tagataaagt ggtgattacc gcaacacaaa tgatggaatc
    23161 catgataagc aatcctcagc ctacccgtgc cgaggtttct gatgtagcca acgcgattct
    23221 ggacggaact gatgctgtga tgctttcggc tgaaacagca agcggccttt ttccagtcaa
    23281 agtcattacc atggtcaata aaatatgttt aagcgctgag aagcatgcca gcttttttta
    23341 tcatagtgat acggaaacct gtcattacca acgggctgac caagcaatcg ctatggctac
    23401 catgcatacc gccaatcatt ttcccattca ggccattatc acactgaccg aatccggaga
    23461 cacggcttta tgggtatccc gacaccacag caccgttcct atttttgcta tatcagccaa
    23521 taagagaacc attggtcgtt taagccttgt aaacaatgtc tttcctatct acattgattt
    23581 tcatcaattc aaccctgaag gattgaatca acaaattttg catgaattaa tcaaatcagg
    23641 gcaccttgaa aagagaggat atgttttatt aacacgtgga acccaaattg gaatgcctgg
    23701 cggcaccaac tgcatggaaa ttatacctgt tgtctgaatt caagatatcc tgttaattaa
    23761 agcaaggttc tttcataaaa gacaaaaatg attattttaa tggcacttta atcaaattat
    23821 tacctctatg ctactattaa gcatagaggt gatcaaaatg ccggataaaa aaccatcaga
    23881 aactctgtta tcgcagcaaa taaatgaagt aagaggtttg ctgctgcaag cccttaaaac
    23941 tcaattagat gatctggtag ctgaaaaact cattccacaa ccaagtggcg ttccttttgt
    24001 tgattttgag aaagatccac cacaaattgc aactctaaaa aaagtcatca attgtcttta
    24061 tcatgcagag gaagcgttta aaagctggga aagcattgat accagtacgc tattgggtaa
    24121 agccaaagca gcgccaaaat taattcgtgc gctaacccag atatacaaat ccttgggctt
    24181 acttgatgag gcaagccctg aaataagagg tgtcatagca gacaattacc atttacttga
    24241 acctctattt actcaagcgt atgctattgt gcaagagtct ggctggttat cagaattcat
    24301 ggaaatggat gtgaccgata aagcatccaa agtgatcttc caagggatgg atttattagg
    24361 tcccgatatc gataaatggc atgatacgca tccgcttgtt gacgcatttt caaaaatatc
    24421 aaaactggta gaaaccatat ccacactaca agaaaaggac atgccaggaa aagagaaaga
    24481 gcaagcggta gaattggttc gcgccttact tgatgacctg gacaataacc cttttatcag
    24541 caaattaagt atcagtgatt ttgaagactc caaagccata aaggatttat tggaatggtt
    24601 taagaacata caagacgatg gttttgattt tagcaagaaa tctattaagc aatatgtttc
    24661 ctgggcaaat cactatttac ccacattaat cgccttcgct gatcaactgg aacggcaaaa
    24721 ctatttaaaa ccaggcagct tatccaccaa tttatgttct caaatagaac agttgggtaa
    24781 acaagtgaat gacatactgt ctgaacctgg ttttggtatt actgagcgag ttgtaacaat
    24841 agactcattg caaaggcaaa gagagcaaaa aattcataat gctcaggtca aaagtgtgca
    24901 agttatcatg gctactgaaa aacaacaggc ggccactgct gatttttatc ggatcctgga
    24961 agcctataag ggcatgaatc tttcacaaat aacagaaccg gatcggaggg tgctaagaca
    25021 attatatccc caaatacaaa cagcgctggc gcatgccagt ttggatcttg aaaatcagtt
    25081 aacctctgtt ttaaacacta ccggccctga aaaaacccca gctaaaccta aatcatggtg
    25141 ggaattagcc aaagatggtt taggttatgt agccagttat gtggtcaatt acaacgttga
    25201 taaaatcctg ggaaccaggg aatctgttga tcgttttctg tcaagccaaa ttgcgtccga
    25261 gcaatttaag atcctggttg ctgaaaaagc ccgtgaaaaa ttaagaaccg gatcagataa
    25321 aataaaagaa actgcactgc aatatcaagc tgaagctcgt gtcaatgcaa taaaatcaac
    25381 cctgagaaag caacccgctc agatggaagc tactccatcc caatttgttg cagtaaaacc
    25441 ctcaaaattg ctcaatcttc gtggaacact tacagccatt caggaaatgc aattatcgac
    25501 tacagtaaat ggcacaagaa caagtgtcga tactctgatt catcgccatt taccagaaaa
    25561 atatcaagtc tgtttttcaa ctcttccgta taaaatcaat aaaaatgatc ctgaattggt
    25621 gaaacaaatt aaaaccgttg aaaataattt gtttcaatta gaaaaagcgc ttaaagattt
    25681 tgaaaaaatc gatttgagct atgggattat gattcgattg cattatttta tcgcaatcgc
    25741 ttctgcagct tccaaactca aatcaagtgt taaagaactt tcccctgagg gtcaaaaagc
    25801 gctagcccca ctattacaac aaattatgac attaggctta tccttctcag acaccaatta
    25861 taaagctaat gatttgtccg ccttgcaaca attaaaaatg gaaggccagc tgagacctaa
    25921 aagcccatcc gtagcatctc cggatacaat aggaacatta cctgaagaaa ctgtaaagag
    25981 aactgcaaaa aaaccaaaag aaaaaacgac tgaattattg gattatgctg acaaaatcag
    26041 taatgctcga gcattactgt taaataagtt taaaaccacc ttgtctcctg cactagccga
    26101 cggtttaaat ccacaaaaaa aaggcgttcc atttgttcat cttgatcaag acccaccaca
    26161 aatcgcggca ataaaaaaaa tcatcaacag tatgtatcat gcggagtgcg ctcttaagac
    26221 ttggcatggc atagatacca gcactacatt gggcaaagtt gctgcagcac atcaaggcgt
    26281 agcggcatta tcgcaagtgt acaaatcatt tacgttgttt accgaggatt tctcagacat
    26341 tcacaattta attcgtgaga accatgattt aatagcgcct gtgattatcg aagcaaacaa
    26401 gctggttaat cagtatggat gggctcgtca attcagtgag ttagatatag cacaacaact
    26461 gggaagcatt ttaggtcagg gtattaatac agtgcaacct aaaacggatg aaataacgca
    26521 aaccgcttca ctggttaaat tattttcaga aattccaagg ttattgaaca atatttcaag
    26581 ttcattggat caaaaatctg agaaatcaat tgatgagtta cgaatcagca acaaaaaaat
    26641 tgaagccata ggcgctgtat ttgaattgtt ttttgaagaa aatgcttact taatgagcat
    26701 gtttaaagga ttcagcgcta tctctggtct tatcgaatta aataaaaaaa ttcaacgcga
    26761 aggaaccaac ctgcaggaag ccactattcg acaataccag gcatggttaa aagaagggta
    26821 tccccatttg atgatgatgc ttgatgaaat tgagactcgc cactacttaa cccccggcct
    26881 gttatcagca cctattgccc tggaattaga ccaaattaac gacaagctta atgaaattat
    26941 tgaaaccaaa cctgatttta aattacaaaa aattcctctt tcattctata tgggagataa
    27001 gcgcatagag cgcttaatga ctaaaaaaac ggaacactgc ctcgcattgc tgcaaataga
    27061 agaacaaaaa aaagcagtga acactttttt cgggatactt aaacactatt caggccagcc
    27121 gctgtcaaac ataaagccaa atgagctcgc tcaattgcgt cttgcttttg ttaagattca
    27181 atccgcaatg gcaaatagta atttggaatt aagcaatgat ttcgttgctg ctttaaatca
    27241 gcttgaaacg tccccacctg aaaaaacaaa gaaaatcaag gtgacagtct ctcaattgtt
    27301 aaaggtgcag cctgctgttg aaaactattt aaacgaacac aaaaaatcct gtgaattaaa
    27361 aatagcagtc attgacaaag ccatcgagca tgtcaaagac aaatcgttcg agctgaaatc
    27421 tggtacatca aaagaaatca atattcctca gctgcgcaga acatttttag aaactgaaag
    27481 caagaagcct gttgcaggtc ctggggaatt aaaaccaaca tcagtctcat ctcttaacac
    27541 cgttcgtggt aatttggctt acattcaaga attaaaaatg tcatccaccg taggcatgat
    27601 gcgagagcaa ttttctgctc tcaccagaga taatttctcc actcgagtgc aaaaatattt
    27661 aatcaaacct caagacaagc cgttgcatat gataaatgac aacgatccgc tcatggtaag
    27721 gcaaattaaa gaagtcgaaa acggtttata tcacttggaa accgctttaa aacaatttga
    27781 acaaatgaaa aagagtgaca gcctggtatc acaaaccaaa gcactgctag aaattgcaaa
    27841 ccatgctctc caattaaaaa tggcggtgga acgattttca cctgagttaa aagagcatta
    27901 tggcccatta gttcggactg tatttagttt cagtaataaa attcaatcca tcaattacaa
    27961 caaagaagat tgggctgatc ttcagtttat tcttaataga gcccataaag aattgttaaa
    28021 aagaaaaaca ccaagagacc atcgtattga aaaagctttt tttgaggccg gaattcgtga
    28081 agtacaaatc gatcgtaatg aatcagtagg aaaaaaagct gcaaaactgg gtgtaaaata
    28141 cgcccatctg gcctcccctc agttagagaa aggaagagcc tatttaagtt ccagatacaa
    28201 aagtgcgttt ggtcaacaac ctaaggttat tcgttcgttt acccgagaac aattagcgaa
    28261 tgaagaattt atggacgctg aaattaacag attgaaaaaa acattagaag agaagtatgg
    28321 ttttaatatt gcaacgatca aagttcttct tgctttatta gagcaaattc aacgggctgg
    28381 ggctcaggct gctgaagtag ctggtatggt caataccttg gttacttatg acttccccaa
    28441 gataaaagaa aatgcgtata gggatttact tacgaaatta tctcaagaag aagactacct
    28501 gaatttaaaa ccaggcacat taattaatcc agccatggct gcagtcaacc aattattctt
    28561 atctgctgcc ttggaattag acatgccttt tagtaaaaag ttatctattc tggacgatac
    28621 cacttatgtc aatattctca taagacaaac cgaaagcgat ctgcaaacat taaagaaaaa
    28681 gctatccgaa gatccctcga atcatgaaat agcatttgaa attgagatta agaacgataa
    28741 attagctttc ctcaggcaac agatggtgtt gcttgaagat aaaaacccca atcaaaccag
    28801 aaatgtctta atggacatgc agttcgaagt gtcattacgt aatcaccttg tttatacaac
    28861 gcttaaagca cccattgccc aggaatatga acaaattgcc agagaatatt acaaaaaaaa
    28921 taaaaatcgc tttctatatg ccgatgactg ttcaaaagag ttgctgcaag gattgaaaga
    28981 atttgaaaaa aaacatttgc ctgatcattt gatagttttt gaagcctacg acaggcttcg
    29041 cctattcagt gcaaaacttc cccctaaaaa tcaggatgtc aaagagtata ttgagaacat
    29101 caatagagaa ttacaaaata gagatatccc tatagagcaa cgtgctttaa aagttaaatc
    29161 attacctaat gactctgtct ttattgaaaa attaagttca gcagataaag gcactcattt
    29221 cctaagaaaa tttatgcagt tcattacaat cattacagca agcatcaccg aagcaataaa
    29281 aaccgggggc agtttgattt atatttatcg ccaaaaacaa atggaacaat cgataaagaa
    29341 tatagaaaaa tcgataagct ttaaagagga gctccatacc ataagagatc ctgatggccc
    29401 ttcaccacgc aagggagaag gaaaagaagg tgaaatcgaa tttaccaaat tgtaagactc
    29461 aataggctat attagtaaaa aatgattcta cactctggac ttaccccgtg aacagtgtgt
    29521 aaacagcaat ggcaaaaata ttcatgttca atgcctaagg ttgttttaaa atcataaagt
    29581 aagcatcgac ataatcgggt aaatc
//
LOCUS       DASLOE010000023        29428 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.023, whole genome shotgun sequence.
ACCESSION   DASLOE010000023 DASLOE010000000
VERSION     DASLOE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 29428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29428
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.023"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            82..1863
                     /locus_tag="U6309_000810"
     CDS             82..1863
                     /locus_tag="U6309_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN5504263.1"
                     /translation="MKIINVPYINVPLILIIIIGGLPLFIQISIKLVQGDLGADTLAA
                     LSLITGVILHEYLAACLIILMLASGQTLEVYARRKASSVLQALISRMPSKVHIRKNEQ
                     ITDIPLSETQIGDEIIIYPHETCPVDGEVIDGHGTMDESYLTGEPYLIAKAPGVTVLS
                     GAINGEALLVVKASKRPSDSRYAAIVEVLKEAEQKKPTLRRLGDQIGAIFTPIALFFA
                     LLAWYFSGDPIRFLSVLVIATPCPLLIAIPITIISAVSMAAKQAIIIKDPTILERLPT
                     CQTAIFDKTGTLTYGKPTLTKIITTETYSHSDILQYAASLERYSKHPLSNSILDAAKE
                     RGLTLLQASEVFEKPGQGLLGKINDHSVQVTSRKKFLAKHPEEVGFLPEHSSGLECLI
                     LIDEQFAAVLQFHDAPREESHSFIHHLAPSHQFKKIMLVSGDRESEVKYLGKLLELTE
                     IYSSQSPEQKLKIVRDETRKAPTLYMGDGINDAPALTAATVGIAFGQYSNVTAEAAGA
                     VIMENTLNKVDELMHLSINTRKIALQSAIGGMLLSIIGMSFAALGFINPVTGAILQEL
                     IDILAIINALQLTWGSRVKIDLPGYKA"
     gene            complement(2166..2459)
                     /locus_tag="U6309_000811"
     CDS             complement(2166..2459)
                     /locus_tag="U6309_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504264.1"
                     /translation="MKKNSSTKKILSSLSDTELQEKQELMMSEQDEQLDILLKTVTRT
                     HEIAEAIQEELTSQNKLIDGLNEHVEKTDGKVKNTTKKVEALIPEVRSSCFLM"
     gene            complement(2487..3386)
                     /locus_tag="U6309_000812"
     CDS             complement(2487..3386)
                     /locus_tag="U6309_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504265.1"
                     /translation="MFSPLQDPWSELEELGFNPEYLKRLKERAATSIEMTKNIEMLAD
                     YYEQFKEVGFTDSDLARIVSLVSFTADNRLANIGSRMMITLVCGGLNLVKAPLHFKPH
                     KLTNMMLSLNGLEKLRTLVEMSKESGFTPLKNQAEFKAIFEKRCKKFPMLRVLWCYSD
                     ANESYMEYRKQDAIYKDWLSEEELHQLNRLSRISKTRDLHYWKRDTVRPVLLGDNYYS
                     REDFGQTREEFEKAQDNPDAVESWCDSWANPFDQNPDSALTQSQEKSNQRNASKIPFF
                     TVYENNGKSGVVDEQQSSNAFSN"
     gene            3548..4987
                     /locus_tag="U6309_000813"
     CDS             3548..4987
                     /locus_tag="U6309_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symporter"
                     /protein_id="HEN5504266.1"
                     /translation="MENKTKALNFLNRIERCLVPQSNPQIMKQLLSALGLDKLTTGNE
                     EYKFFIQTIKKKSIDLNPLISKVKSDILNNDPLWTLLDYIEANELIDDAEIENASSTI
                     QLQINLLCLLEAITITMANSNDFAHDIYNHMMKQRGKGATGNPFYNFLFGIPSRATLF
                     ERLKLISIDPGLTSIIFHRQMGENPETQADLDDFVSQNSLSGWNADIELANFDVASTS
                     TLPAMGVNILEAVWEDATGHNKNTGGILNAQAGLGLIRVMEEQEYTTSFKLAETTLPQ
                     GTTLHSDFGYSLIPELKVDNSPKKVSQFHIDKQWMNLYNSWNLLFVIGNIDSVFVPLK
                     LLMPSVFSAEPSNYKETRVLTLFLVANLFLNQQTLNNPLFSNNFSFRNGTVIMKKWGE
                     INKNHASELLQQCKELDDLYNHVLGKNPHLNFGLKLFSYFQDSHQFRLTKKAQPSTSI
                     DYSFFSGNTPSENNSEKMGVALHSSLYTK"
     gene            5233..6141
                     /locus_tag="U6309_000814"
     CDS             5233..6141
                     /locus_tag="U6309_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN5504267.1"
                     /translation="MNILSCLKGFITTVDCKSFSKAAQKLYISPSKLSKQITWLEEEL
                     KVTLLIRSTTRLTVTDSGHRLYEKSLQLFEQLEAVKVIATPDQLELNGVIRLYLTVTP
                     AIPYLTTLSIQFMRQYPKIEVDIIVGSDTKELYSSTFDLAISFDDVKHSKLVCKRLFS
                     IQRKVFASPGYIAQHGLPQTLDELPKHNCLINTLYGLQNKWVFNKKIIHVSGNFKSNN
                     AGVLKQAAVADVGILWAPYFSVYEEIKNETLIPVLPQESSPDINLYAICPKSTVDEQK
                     INLLLNFFCEKALSENIATSFIDGNN"
     gene            complement(6496..6942)
                     /locus_tag="U6309_000815"
     CDS             complement(6496..6942)
                     /locus_tag="U6309_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEN5504268.1"
                     /translation="MKSEQSTWLAVFDSTTCSIYDYSKSDLSLVKKIEHSENKLKDID
                     ITSDRPGRYQASGAAHGAFSQESDPKEIQIERFSKEIAKILEHGSAVNAYEKLILASA
                     PHMHGLLLKNINRQVEKSIIHVIPKDLIHFSENDLLDYVNNELKPK"
     gene            complement(7525..8241)
                     /locus_tag="U6309_000816"
     CDS             complement(7525..8241)
                     /locus_tag="U6309_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEN5504269.1"
                     /translation="MEFLRRNRVGLVIIAILTCLFLILIPKQLSKKYKIVSQTPFLIE
                     IKLPDPAYSSPISIEEALKKRRSIRQYKNQPITLQQASQLLWAAQGITSDTGLRTAPS
                     AGACYPLELYFIASNVSNLSPGVYHYEPTKHTLKQLKAEDISSQLVQAALGQQAVKSS
                     AANIVITAVFSRMTKKYGKIGRRFALMEAGHVAQNIYLQSVSLNLATVSIGAFDITQV
                     RESLGIKDMEPVYILPVGAK"
     gene            8576..9745
                     /locus_tag="U6309_000817"
     CDS             8576..9745
                     /locus_tag="U6309_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504270.1"
                     /translation="MIQRIRRYAKKIYEDKFRFILIILILLLIAPMLLISQPKTVEGK
                     GMEMHFFYTPSCPYCAKQKLFNKRLLKNYKVKIVSHDITDPIEKKLFIFIAKQHGITQ
                     HNLGVPLTIVNGKAIIGFESQESSGRLIEQALEQGDNKQPLSQIKPVETQKIPILGEV
                     DITHYSLPTLAMLLGLIDGFNPCAMWALVFLIGLVTGLHDARKLWLLVGTFVFSSGIL
                     YFLFMTAWLNAFLLIGYIRPVVILIGAFAIILGILQIRDFFTKDITCPIPTQQKQKVM
                     RRMKILVLSPLTITTITGIIILAFVVNLIEFVCSSFIPAIFTQALALHELQTWQYYFY
                     ILLYDIFFMLDDLIIFSFAVFAVNRISTQYARWCKLIGGITLFVLGIVLLTTSNS"
     gene            10000..11889
                     /locus_tag="U6309_000818"
     CDS             10000..11889
                     /locus_tag="U6309_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN5504271.1"
                     /translation="MPSTRNIETHNDSNPTLRALSLSALGIVYGDIGTSPLYTFKTVI
                     LLAGGGTPDVTTIMGSVSLIIWTLIIIASVKYISFALRIDNDGEGGILALMSLLSLKL
                     KQKPFIIAVGLMGAALIYGDGTITPAISVLSAIEGLEILSPSLKYYVLPIAITILITL
                     FAIQSKGTATIGKAFGPVMAFWFLTIGILGARGVIQHPFVLAAINPVYGLNFLFSNGA
                     TGFFILCGVFLCVTGAEALYADLGHFGTAPIRCAWFGLAFPSLIFNYLGQAALVLEGA
                     STEHNIFYMLCPSDFLLPLIILSTVATIIASQAIITGAFSMTRQAMQLGWLPRLRVTQ
                     TSSEGYGQIYIGVVNWFLMLATLGLTIRFGSSEKLAAAYGIAVSATMLCTTVLLFIAL
                     HKLWKWNIITSGLVAGLFMIVDASFFAANLTKFINGGYIPITLAIIIYSMMYIWHKGY
                     KTIAIKQKEKNITVDSFLESIQKEGVVRVPKTAVFLTSKEQDIPPILVWHVKKNHVLQ
                     DKVIILNINNLSIPWCKPADQLQIVETGAGIWHAVANYGFMEQPHIPKLLKKLEAQGY
                     DINIKDITYYIGHETIFVRNVRHTMSKYIKILFVFMHRNALPMSNYFHLPPESVFEIG
                     RQIEI"
     gene            12107..13870
                     /locus_tag="U6309_000819"
     CDS             12107..13870
                     /locus_tag="U6309_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504272.1"
                     /translation="MVQFAKPSIGPPADCGRINLLIGRELGQYQNIIQKWIANLEDNA
                     KQKPSNISEFKFGKILGYLLLQSLKKANLHPDNFRSFGSDPWEEPHYQSARIAGKYVV
                     TQDTYRIYFDIPRIDHSDNEKIKANQDHAQILYFTTMTNDGLMVFSFEEKVEKIEPKL
                     FVELAKNIEVESNAWAQLIDFTEKNFHTREDLNSNPQVPQILGLILYATIFPEHRNEA
                     FNGLCPLLLKSENPNFNSITTKLLKDIIPDYEAKAMAFLHKNNNPMRYSERDISEQGA
                     LLGLSDEKIDVVQNEMRERKASEVQNNNRSQAIELKKTLIGAVDEYLRWRNNESKETD
                     YQKSSGAFTWLRHYSDFGKNRANDLKNELNKAQDLKTMLDVLQKHFANNSRLHNHSLD
                     SYLLRAVYKDFNKFNSIFNFEHLTIKNDTDANREWLREEMLGMAAKTNMNVTLENPTV
                     SRQESPTLPNKAKIRISIMKIPTNEREENILAVHTLLRNDELLRNQDGSVPTIIKEIR
                     DIVGKIDPSEEENIAHAIIEIKGKIAGNSDHNYNENALDVIKAFESPSCCDFRKIRAA
                     LTTNHAINEIMNPVRVGMQLN"
     gene            14122..16860
                     /locus_tag="U6309_000820"
     CDS             14122..16860
                     /locus_tag="U6309_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEN5504273.1"
                     /translation="MEEQQHISAPGWHEQSVEKTLIMLNTTERGISEQESTKRLEYYG
                     FNRLPEPKRKSIFLRFLLQFHNILIYVLIGAAVVTSFLQHWVDTAVIIAVVIINALIG
                     FIQEGKAEKALDAIRKMLSPAATVLRDGERRHISGERLVPGDIIFLEAGDKVPADVRL
                     VRSHGLRVQEAILTGESIPVEKQIHPVAKNTELGDRTCMAFSGTLVTNGQGKGIVVAT
                     GSSTQIGHISGLLSKIESLTTPLVLQIGRFAKWLTLFILLIAALLLFYGYFIQNHPFA
                     ELFMVVVSLSVAAIPEGLPAVLSITLAIGVQTMAQRHTIVRRLPAIETLGSVSVICTD
                     KTGTLTMNEMIVSSVLTSNHLFILDGIGYEPQGTITLNEQVIDNNEYPLLKKLARAAI
                     LCNDAALHNQEGNWTVEGDPMEGALLAFAGKTGLAMQEENVLWVRTDVIPFDAKHRFM
                     ATLNHDHLQHAMIFVKGAPEQILKMCQNQQINQGEVQPLNEPYWKRQMEQVAARGQRL
                     LAFAVKKTKPEHTVLEFADIKDGLTLLGMVGLIDPPRPEAIEAVAQCHTAGIQVKMIT
                     GDHASTALAIGRQIGLKNLDKVLTGIDLENMDDAILKNAVLETDIFARTSPEHKLRLV
                     MALQSHGMTVAMTGDGVNDAPALKRADAGIAMGKKGSEVAKEAAEFVLVDDNFASIVA
                     AVREGRTVYDNLKKVISWTLPTNAGESMTVILAILLGLSLPVSPIQILWINLITAVTL
                     GIALAFEPTEESTMHRPPRPRNEPLLTGSLVWHIILVSILFICGIFGLYFYALDRGYS
                     IELARTIALNTLVVLEIFHLFYIRNIYSTSLTWKAVQGTKVVWIAIIVVTVAQFAITY
                     FPPLQTIFVTESVPFGDGVLIVLTGATFFAVIEIEKQLRLRLRVISPPSAREKCSL"
     gene            17025..17588
                     /locus_tag="U6309_000821"
     CDS             17025..17588
                     /locus_tag="U6309_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504274.1"
                     /translation="MTIYFLFGKTGSGKSYIGELLRQLNIIHIDGDNHITQKMRDCLI
                     EDEQMTPEMIDEFVDVLIDVINTQKEKTPKESFAISQAMYLDKHRIKLLNAIPELKFV
                     MIDVTPVLRASRITNRFRNQESKVSLRYATKMDTFFEYPSHETIRLENNQGSDETLIE
                     QIREKLPALFNMDSKEELNPGIKLQFA"
     gene            complement(17703..18629)
                     /gene="leo"
                     /locus_tag="U6309_000822"
     CDS             complement(17703..18629)
                     /gene="leo"
                     /locus_tag="U6309_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease Leo"
                     /protein_id="HEN5504275.1"
                     /translation="MKNKIVLLAPVSLSFLFSFPTYAADYEFNGLSLSASLGILSGNA
                     HEYVYHPETDTKLSQLDWRIKNAPIIKGELNYDVLDWLSMNGRGWTTLVKNKAAMDDY
                     DWFNPYQETWTHWSHHEDTYLNYANELDMSLRAWLMQKQNYKLGLATGYQWNSFDWQA
                     TGGCYQYNGGLYAGCFPSDQPGIGYQQKFRTPYIGLAGKYFINNFEFSGLLKFSDWVS
                     ARDHDEHYARNLTFNERGNSSRYYAATIHSAYFLKPNTKIFVEASYNHYSNGRADTEI
                     IDNDTGERSYFGDSAGLSNKHYTVALGLQYLF"
     gene            complement(18757..20364)
                     /locus_tag="U6309_000823"
     CDS             complement(18757..20364)
                     /locus_tag="U6309_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN5504276.1"
                     /translation="MNKNLSALSLLWISITSMVGSGWLFGSLYSAHFAGPAAIIAWPL
                     AGFLLLFVALSYAELGTMFPQSDSLACLPLYTHGRLTGIIMNVMTWFSLAILPVIETQ
                     GVIQYASNYIPDLVSHDALHYRNTPAGYMLSLVVLMSFVLFNYFGIGLFARINAAFTI
                     WKIIIPILTIASLVTMSYHPGNFFQHGGFMPYGWQGVMAAMSNGGVLFSLLGFRQVVI
                     MMGELEKPGQYIPLILGGSLVFVTLLYTGLQWSFIGSVSEQALEKGWVNLSFTGDAGP
                     FAALAALAGMLWLSILLYADAFISPYSTGLVYSTTAAHMLSSMGSVVPAPVILTKQNR
                     YQVPWVSLGVNFLLAATMSLVLHGWQEMSAFLVAILMISYSIGPVALVCLRQQLPHYK
                     RPFRLQGSNLIGFLGFYVCTVGVYWAGFHSVRKLLVITILGVVCSFLYCLMKNTHREI
                     DSKQALWFIFYLFGLGIFSFLGNYGGKHILPQYWDLLYLLVFSLLVFGFALLSRKPSA
                     YTQNMIAQNTHTNITAPAFSIERYDIA"
     gene            20695..22860
                     /gene="katG"
                     /locus_tag="U6309_000824"
     CDS             20695..22860
                     /gene="katG"
                     /locus_tag="U6309_000824"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEN5504277.1"
                     /translation="MDGKVGNTATGCPVMHGGMTSAETSNTAWWPNALNLDILHQHDT
                     KTNPMGKDFNYREEVKKLDFEALKKDLHALMTDSQPWWPADWGHYGGLMIRMSWHAAG
                     SYRVADGRGGAGTGNQRFAPLNSWPDNVNLDKARRLLWPIKKKYGNKISWADLIVLAG
                     TIAYESMGLKTFGFGFGREDIWHPEKDVYWGSEQEWLGAKRYDGKSRESLENPLAAVQ
                     MGLIYVNPEGVNGQPDPLRTAQDIRVTFGRMAMNDEETVALTAGGHTVGKCHGNGNAK
                     FLGPDPEAADVEDQGLGWINKTTRGIGRNTVSSGIEGAWTTHPTQWDNGYFYLLLNYD
                     WELKKSPAGAWQWEPIHIKEEDKPVDVEDPAIRHNPIMTDADMAIKMDPVYRKIAERF
                     YKDPDYFAEVFARAWFKLTHRDMGPKTRYIGPDVPKEDLIWQDPVPAGNRAYDIAAAK
                     AKIAASNLTIGEMVSTAWDSARTFRGSDKRGGANGARIRLKPQKDWEGNEPQRLTKVL
                     RILEGIAADTGASVADVIVLAGNVGIEKAAKAAGFDIIVPFAPGRGDATDDMTDAESF
                     DVLEPLHDGYRNWLKKAYDVRPEELMLDRTQLMGLTAHEMTVLVGGLRVLGTNHNNTQ
                     HGVFTDRVGALTNDFFVNLTDMANVWIPSKDNLYEIRDRKTGNIKWTATRLDLVFGSN
                     SILRSYAEVYAQDDNKEKFVKDFVAAWTKIMNADRFDLA"
     gene            complement(23171..24448)
                     /locus_tag="U6309_000825"
     CDS             complement(23171..24448)
                     /locus_tag="U6309_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504278.1"
                     /translation="MKNNKRTNDQNADQKSSQYIYVEKKEIGGIDTEIITESHYPIPW
                     GSASERPLDYYEKQSKIITYYKEDIDLDGNPFSKRIEIEIPEVEEGENRHRRIKVKID
                     GNPIIFQDNNILDMDETFNIRDVNLLMHMVLNNFHSKSNSTRLQTITPFIHLPETAGD
                     KDYKFNQKNNGDISKILLPYVQEMCSLNNSSVVRELLIPVATKGGPHHWNLCMLSIDE
                     NNTPSLYYIESENLLVSFDYYTYMYYMEDYKMHILPQVNALLAKFHYPAIDKINFCQA
                     KQFSQEGCGIAMGLNMQKILEENYPQVICHSSTQRKEVPSHEEIRRLSSNVSIEEDAL
                     MRVELAFKIQARASIIAEGALDAKMVEKKSLVCSEILSDSRSLNVKAKEAFGFFQSAQ
                     PGISSEMHSVMAQDKGNDEASSSNINAGACRLW"
     gene            complement(25008..26240)
                     /locus_tag="U6309_000826"
     CDS             complement(25008..26240)
                     /locus_tag="U6309_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5504279.1"
                     /translation="MDKKIIAAASFGTLFEMYDYAIYGFMAPILAPLFFPATDKHTAL
                     IATFGIFAAGFLVRPFSAYWLGNLGDKIGRKKTLTMTLMVMTFSSSFIGLLPAYQEAG
                     ILAPILLTLCRLTQGVSVAGEMTLASIYIYENVNMRHKGLAVSFVTLGSICGLLLAII
                     VSKITLIFFSSQSPDFLWRFPFLIGIFVGFIGLYLRSQTRELTDFAKVIVQRQSHPIL
                     NALRYRKKAIAAIFLLAIYCNSSFYTFFVYMPSYLQQQELPLTTAMDVIIIGMICYSM
                     GLLLSGFTSDILGRRFPLAVSLIATLCTVLVINYLINTGTLLHITVACSVLAVIISFF
                     IGSAFTASLEQLPQEERASGFTLFFNLSNSLFGGTAPLIILLLSYVFIHFAFSIYLMV
                     MALLTLPALWLIQEKGTN"
     gene            complement(26281..27585)
                     /gene="sdbC"
                     /locus_tag="U6309_000827"
     CDS             complement(26281..27585)
                     /gene="sdbC"
                     /locus_tag="U6309_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbC"
                     /protein_id="HEN5504280.1"
                     /translation="MPNSSIQIWLQRQIALRTFPPNREDWYVKNGFGNSEMSLEERFA
                     CFVEAIRQEDSPYHEQLSGIDFHRFEIEVTLANQKTCMTEVIGFQNNSNQSSSLLNEK
                     VKAVGKGKHVIYFTGIGTQYQDCLIDIAKAVQITGAHYYAFEYPGMRKLGGEVLEVND
                     MVNTGIALANTLLEKGISIDDILFQGDSFGAAVAKKVSDQFKLQSNVTVRCILNNTFS
                     TFQAAVEGNLGAFSTLRYTVRPLLRYTGWDIRPGDNYGLDTPYQIHINHTGDLTLGAG
                     HATLAESVESNSQLEHFVDPCPEEYRIKRDIYKQWHWASLSEQGIRFLETKYGRNSDG
                     QLDTHLADLYLLKYDTGLGVYESFICKYLEDSNDYIANHPQKLSLEELPSPLESETVS
                     LGQLIVSVLPSRPSIAGFFGRPKPVKDEVDSENQAQKSIELK"
     gene            27853..28737
                     /gene="legL6"
                     /locus_tag="U6309_000828"
     CDS             27853..28737
                     /gene="legL6"
                     /locus_tag="U6309_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL6"
                     /protein_id="HEN5504281.1"
                     /translation="MFYELILTRTSNLIQEFISIPNGVTSLDLSLNELGNISNAELTQ
                     AFQYIPDSVISMDLANNHLCDKSGSELAQLLAAIPANVTSLNLSCNDLHKKSGAELAQ
                     AFAAIPASVTSLNLHCNYLGNNRGVELAKAFAAIPENVTSLDLSMNYFDLESSADLSQ
                     IFTSIPPHVASLNLSFNSLHEVPFEKLVLLKDSLKHVQTVYLSFYSVKEMSKEQRRAL
                     GAAFPNAQKIILIDDYGHEIQPSIIISNLIRELSGKADAPSLLNQCILFTQRHQKDSD
                     NKIIPKELEESIRTFNSH"
     gene            complement(28857..29321)
                     /locus_tag="U6309_000829"
     CDS             complement(28857..29321)
                     /locus_tag="U6309_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEN5504282.1"
                     /translation="MKHRRSETQDIDDAIIKTLNKLLEIELAGVVRYTHYSFMVFGFN
                     RIPICKWMRDQAQESLNHAHLIGELITHFSYHPTLKIGKLLETHEHNISNILEESLEH
                     ERQSLDLYYQLLQYSENKSVLVEEFARQMIQEEEMHLGEISKMLNSPTTFKD"
BASE COUNT         9223 a         5770 c         5506 g         8929 t
ORIGIN      
        1 tacttttttg atgcaatcaa aacaaaatgg caattttgga tcactattta ttcaatcatt
       61 gccatagttc tttatatcat aatgaaaatc attaatgtcc catacattaa tgtgccttta
      121 atccttataa ttattattgg tggcttacca ttatttattc aaattagcat taaattagta
      181 caaggtgatt taggtgctga tactctggct gctttgtctt taataacagg cgttattttg
      241 catgaatatc ttgcggcctg tttgatcata cttatgcttg caagtggcca aaccctggag
      301 gtttatgcaa gacgcaaagc ttcttctgta ttacaggcac taatatcacg tatgccatct
      361 aaagtacata tacgtaaaaa tgaacaaatc acagatatcc ctctttcaga aacacaaatc
      421 ggtgatgaaa ttataatcta tcctcatgag acatgccccg tagacggtga agtaattgac
      481 ggacatggta caatggatga atcatatcta accggtgagc cctatctaat tgcaaaggca
      541 cctggcgtga ctgtattatc aggagcaata aatggggaag ctttattagt tgttaaagcg
      601 tcaaaaagac catcagactc tcgatacgca gccatcgttg aggttttaaa ggaagccgaa
      661 caaaaaaaac ccactttacg ccgtctagga gatcaaatag gggctatttt tacaccaatc
      721 gcattatttt ttgccttgct tgcctggtat ttttctggtg atcctattag atttttatct
      781 gtattggtca ttgcgacacc ctgtccattg ctcattgcaa ttccaattac tataatcagc
      841 gccgtttcga tggcagccaa acaggcaata attataaaag atcctactat tttagaacga
      901 ctaccaactt gtcaaaccgc tatatttgat aagacaggaa cattaaccta tggcaaacca
      961 actttaacta aaatcattac cacagagact tacagtcact cagatatctt gcaatatgca
     1021 gcaagccttg agcgctattc taagcatccc ttgtctaatt ctatattaga cgcagcaaaa
     1081 gagcgtggtt taaccttatt acaggcatcg gaagtttttg aaaaaccggg acaaggctta
     1141 ctcggaaaaa tcaatgatca ctcggttcaa gtaaccagtc gcaagaaatt tttagccaaa
     1201 catcccgaag aagttggatt tttgccagag cattcttctg gtctggaatg tcttatattg
     1261 atagatgagc agtttgcagc agtgttacaa tttcatgacg caccacggga agaaagccac
     1321 tccttcatcc atcatttagc cccttctcac cagtttaaaa aaatcatgct ggtatccggg
     1381 gatcgtgaat cagaagtcaa gtatttgggg aaactacttg aattaactga aatttattca
     1441 tcccaaagtc ctgaacaaaa acttaaaatt gtcagggatg aaactcgaaa agcaccgact
     1501 ctttatatgg gcgatggaat caatgatgca ccagccttaa cagctgcgac tgtcggcata
     1561 gccttcgggc aatacagtaa tgtcactgca gaagctgcag gtgctgtaat tatggaaaat
     1621 accctcaata aagtagatga attaatgcac ctaagtatta atactcgtaa aattgcattg
     1681 caaagcgcta ttggcgggat gttactcagc ataataggaa tgagctttgc ggcccttggc
     1741 tttataaatc cagtaactgg ggcaatactt caagaattaa ttgatatact tgcgattatt
     1801 aatgctctgc aattaacttg gggtagtaga gtcaaaattg atttaccagg ttataaagcg
     1861 taaaaaatta tatattttcc tttttccgat gaaccattaa tataatacct ttgcgtgagg
     1921 tttgcgtact tgagtacgac actgttatac ataatacgat aattgtagca ggatctcatc
     1981 acaaattatc aatttaaatt ccatatgaga gtggcatttc ctgacagcgt tttagatcaa
     2041 tttttgactt cagaaacaca atgaaaaccc gggatacgga ctacccctcc tcccaggcta
     2101 cttatcgatc atacgacgca aggcttttct tctttggggc tgcacccaca gtcaattcgc
     2161 caggattaca tcaagaaaca gcttgacctt acttcaggga taagagcttc cacttttttt
     2221 gtcgtatttt ttactttacc atcagtcttt tccacatgtt catttagtcc atcgattaat
     2281 ttattttgac tggtcaattc ttcttgtatc gcttcagcga tttcatgagt tcgagttaca
     2341 gtttttaata aaatgtccaa ctgttcgtct tgctcgctca tcatcagctc ttgtttttcc
     2401 tgtaattccg tatcacttaa agaagataat atttttttag tactgctgtt tttcttcatg
     2461 attgcaacct tattttattt actttattaa tttgaaaagg catttgaaga ttgttgctca
     2521 tcgacaacac cactttttcc attattttca taaacagtaa aaaaaggtat tttactggcg
     2581 tttctctgat tcgacttttc ttgtgattgt gttaaagcag aatctggatt ttgatcaaaa
     2641 ggatttgccc atgagtcaca ccaagattcc actgcatctg gattgtcttg tgctttttca
     2701 aactcttccc gtgtttgtcc aaaatcttct ctggaataat agttatcgcc aagtaaaact
     2761 ggtcttactg tatcgcgttt ccaatagtga agatctcgcg tcttagatat tcgagataat
     2821 ctgttaagtt gatgcagttc ctcttcgctt agccagtctt tatagattgc atcttgtttt
     2881 ctatattcca tataggattc atttgcatca gaataacacc ataaaactct gagcattgga
     2941 aattttttac atcttttttc aaaaatcgct ttgaactctg cctggttttt aagtggtgtg
     3001 aacccacttt ctttgctcat ttcaactaat gttcgcaatt tttccaaacc atttaaacta
     3061 agcatcatat tagtcagttt gtgcggcttg aagtgaagag gagctttgac taagtttaat
     3121 cccccgcaga ctaaggttat catcattcgg cttccgatgt ttgccagacg attatccgca
     3181 gtgaaactga ccaaactcac aattcttgcc agatcgctat cagtgaaacc tacttcttta
     3241 aattgttcat aatagtctgc caacatttct atatttttag tcatctctat agaagtagcg
     3301 gctcgttctt taagacgttt caggtattct gggttaaaac caagctcttc taattcggac
     3361 cacggatctt gtaatggaga aaacatataa atcaccttaa atcagttaaa aaggacaaaa
     3421 aatatatcac gtgattattt attttactat ttccaattta gaaaccgtta ttttccaaat
     3481 gaatacattc tctaaaaaat caatatgtgt aaaatagaca cacttagact taatttagga
     3541 atataatatg gaaaataaaa caaaggcatt aaattttctt aaccgtattg aacgatgtct
     3601 tgtaccccaa tcaaatcctc aaataatgaa acaattatta tcagcccttg ggcttgataa
     3661 gctaactact ggtaatgaag agtataagtt ttttatccaa acaattaaaa agaaaagcat
     3721 tgatctgaat ccgttaatta gcaaggtcaa atcagatatt ttaaataatg accctttatg
     3781 gactttgctt gactatatcg aagcaaacga attaattgat gacgctgaaa tcgaaaacgc
     3841 ttcttctacg atacagcttc aaattaattt attatgcctt ttagaggcga taaccattac
     3901 aatggctaac agtaacgatt ttgctcatga tatttataac catatgatga agcaaagagg
     3961 aaaaggcgca acaggtaatc ctttttacaa ttttttattt ggaatacctt cacgtgccac
     4021 cttatttgaa agattaaaac tgatttccat agatcccggt ttgacaagca ttatatttca
     4081 ccgacagatg ggagaaaatc ccgaaactca agctgatctc gatgattttg taagtcaaaa
     4141 tagtttatct ggatggaatg ctgatataga acttgctaat tttgatgttg catcaacaag
     4201 tactttaccc gctatgggag ttaatatttt agaagcggtg tgggaagacg caacaggcca
     4261 caataaaaat accggcggta ttttaaatgc acaagccggt ttgggattaa ttcgcgtcat
     4321 ggaagaacaa gaatacacca cttcttttaa attagctgaa actactttgc ctcagggcac
     4381 tacacttcat tcagattttg gttattcatt gatacctgag ttaaaagttg ataactcacc
     4441 taaaaaagta tctcagtttc atatcgataa acaatggatg aatttataca atagttggaa
     4501 tttattgttt gtgattggaa atattgattc tgtatttgta ccacttaagt tgttaatgcc
     4561 cagtgtgttt tcagcggaac catcaaatta taaagaaacg agagtgctca ccttatttct
     4621 tgttgctaat ctcttcttaa atcaacaaac gcttaataac cctctgtttt cgaataattt
     4681 ttcctttcga aatggaactg tcattatgaa aaaatggggt gaaatcaata aaaatcatgc
     4741 cagtgaactc ttgcaacaat gcaaagagct ggatgattta tataatcacg tactcggtaa
     4801 aaaccctcac ttaaactttg gattaaaact tttctcatat tttcaggatt cacatcaatt
     4861 tagactcact aaaaaagcac aaccaagtac atccattgat tattcatttt tctcaggtaa
     4921 taccccatct gagaataatt cagagaaaat gggtgtagcc cttcatagtt ctttatatac
     4981 aaaataaaat gttatgcttt atacccagca aataaatagc agaatcggag acaaattata
     5041 gatggtctcc gtttttgaga aaaaatttct ttctgaaatc tttcacgggg atacttgacg
     5101 atccgtttta actgctgttt tcatggaata aactaatagg gttggtctat gtcagtaggt
     5161 atgcggttga tttacaaaac tggtatatag gtctatacca tctaaaaaga gctcgattaa
     5221 gaaaatttta agatgaatat tttatcctgt ttgaaaggct ttattacaac cgttgattgc
     5281 aaaagctttt caaaggcagc acaaaaattg tacatatccc cctctaaatt gagcaaacaa
     5341 ataacctggc tggaggagga attaaaagtt accttattga tacgctctac gacacgatta
     5401 actgtaactg attcaggtca taggttgtat gaaaagtcat tgcagctttt tgagcaatta
     5461 gaagcagtca aagtcattgc aacacctgat cagctcgaat taaatggcgt catcaggctc
     5521 tatcttactg ttactccggc aattccctat ttaaccacat taagtattca atttatgcga
     5581 caatacccca agattgaagt agatattatt gtcggttcgg atacaaaaga gctttactcc
     5641 tctacctttg atctggctat ttcatttgat gatgtcaaac attcaaaact ggtttgcaaa
     5701 aggttatttt caattcaaag aaaggtcttt gcctcacctg gttatattgc tcaacatgga
     5761 cttcctcaga cactcgacga attaccaaag cataattgtt tgattaatac tctctatggc
     5821 ctgcaaaata aatgggtttt taacaaaaaa atcattcatg tttcaggaaa ttttaaaagc
     5881 aacaatgcgg gtgtcttaaa acaggctgca gtagcagacg tggggatttt gtgggcgcct
     5941 tatttttcag tttatgaaga aataaaaaat gaaacattaa ttccagtatt gccccaagaa
     6001 tcttcaccag atatcaatct ttatgcaatt tgccccaagt ccactgtgga tgaacaaaaa
     6061 attaatttac ttttgaactt tttttgtgaa aaggccttgt cagaaaacat tgcgacatcc
     6121 tttattgatg gaaataatta aagatggatt agaacttttg actttgatac tcagaaaata
     6181 cacaagaatg attgtattac cctaaaactt cagcagacgc atggaattta gggttgtgtg
     6241 atgaatcttt aatgagttag taccatttga caatcaaaat tcaagccatg tccttcaata
     6301 cttgaggaag caaggataaa ttagacactt ttcaaccgtt tattaggcaa agccaaaaat
     6361 aatgtcttat ccactctgat tctgatagaa cgtattgtta ccatactgaa accattcaag
     6421 agcagaaaat cctgtgtcct atttatttaa cacgtatcaa atttactcaa aactcacttg
     6481 ttaactggct tcctgttact ttggcttcaa ttcattattt acataatcta aaagatcatt
     6541 ttcagagaaa tggattaaat ctttaggtat tacgtgaatt atcgactttt caacttgcct
     6601 gttgatgttc tttaatagaa gaccatgcat atgcggcgcg gaagctaata tcaatttttc
     6661 atatgcattc acagcgctac catgttcaag tatcttggca atttcctttg aaaacctctc
     6721 gatttgtatt tctttggggt ctgattcttg gctgaatgca ccatgagcag cccctgaagc
     6781 ttgatatcga cctggtctat cagaggtaat atcaatatcc tttaatttat tttctgaatg
     6841 ttctattttt ttaactaagg ataagtcact ttttgaatag tcatatatgc tgcaagtagt
     6901 ggagtcaaaa actgcaagcc atgttgattg ttcggatttc atattacctc catgcctgga
     6961 atgagaattc catctatgaa ctttaataac atcaaaagtc aaacagctat cacctatcaa
     7021 gatgaccaag cctatatttt tgtgtcaata tttgacttgg gctttataaa attgcctcgt
     7081 agtatgcttc tgcctgataa atattacctt ggagccaaat tatcaactta tttgaataag
     7141 tccaaaatag ttggatctca tgcaaataga gcaagccata tccatatttt tttcaaggcg
     7201 tacaaattat ttgtttttta agtatagcat tttctttgtt caacaattat ttttgcattt
     7261 ttaaatgcag atcaccgcaa atcaacgaag cttaacccat gccagtctat ggagcagccc
     7321 agacatttca attcatttca acaataagat aactggtatg ccattatgga ttgggctttc
     7381 aaaccaatag ctaacccgat ttagagcaat ttcgactcta ccaatctctt caatttttca
     7441 gagacaaagt ttcccataac tcttctctgc aaacggtttg attgttaaag cattagaaaa
     7501 tcttaccttt ataatttata tttttcattt tgctcctacc ggcagaatat atacaggctc
     7561 catgtcttta atacccagag actccctgac ttgagtaata tcgaacgcac ctattgatac
     7621 agtagccagg ttgagagaca ccgattgaag gtaaatattt tgagcaacat gccctgcctc
     7681 cattaaagca aaacgcctcc ctattttacc gtattttttg gtcattcgag aaaaaacggc
     7741 tgtgatcaca atatttgcag cgctcgattt tactgcctgc tgccctaatg cggcttgaac
     7801 taattgtgaa gagatatcct ctgcctttaa ttgttttagt gtatgctttg ttggttcata
     7861 atgataaaca ccaggtgata aattgctaac attacttgca ataaaataaa gctccaatgg
     7921 atagcacgct cctgctgaag gtgctgttcg caaacccgta tcagaagtta ttccttgcgc
     7981 tgcccaaagt agctgagaag cttgctgaag agttatgggc tgatttttat actgcctgat
     8041 agagcggcgt tttttaagcg cttcttcaat tgaaatgggg cttgagtaag ctgggtctgg
     8101 taattttatt tcaatcagaa atggagtttg tgagacaatt ttatattttt ttgacaattg
     8161 ttttggaatt aaaatcaaaa aaagacaggt caagatcgct attattacga gccccactct
     8221 gttgcggcgt aaaaattcca tttactatcc ccctggtaat aactcccaaa ataagatatt
     8281 tattttttgt accgtttttc tcctggattt tgatattcaa tagaaccctt tattctgtat
     8341 gctgtcaatg ttgatttaaa aatgaacttt tggggaaatc gcttcgttac ccaaattttt
     8401 aactgtctga ttttgttggt tgggattttc tttttgataa caatagaatg tgaaatttta
     8461 ccaaactcgc ttgatccgtg aaggtgtgac gtaaggaatg cagggaattt ttgctcaatt
     8521 ctcataaaag atattgattg gcatcaaaac tatctttaag aattaaagga acataatgat
     8581 tcaaagaatt cggcgctatg caaaaaaaat ctatgaggat aagtttcgat ttattctaat
     8641 aattctcatt ctattgctca tagctccgat gctattgata tcccagccca aaacagtgga
     8701 aggcaaaggc atggaaatgc attttttcta cactccaagc tgtccatact gcgccaagca
     8761 aaaactattt aacaaaagat tgctaaaaaa ttataaggtg aagatcgtca gccatgatat
     8821 aacggaccct atcgaaaaga aattatttat ctttatcgcg aaacaacacg gtatcacgca
     8881 gcataatttg ggtgttcctt taaccattgt taacggcaaa gccattattg gttttgaatc
     8941 acaagaaagt agcgggcgtc taattgaaca ggctttggaa caaggtgata acaaacaacc
     9001 cctctctcag ataaaacctg tcgaaacaca gaaaatacca attctgggag aagttgatat
     9061 aacgcattat tctttaccca cactcgcaat gcttcttggt cttatagatg gttttaatcc
     9121 atgtgcgatg tgggcattag ttttcctgat aggtttggta acagggttac atgatgctag
     9181 aaaattatgg ctattagttg gaacatttgt gttttcctca gggattttat attttttatt
     9241 catgaccgcc tggttaaatg catttctgct cattgggtat atacgccccg ttgtcatttt
     9301 aattggtgca tttgcaatca ttctcggaat tttgcagata agggattttt ttaccaaaga
     9361 catcacttgc cctattccaa cccaacaaaa acaaaaagtc atgcgacgca tgaaaatttt
     9421 agtgctttct ccacttacaa taacaacaat taccggcatt atcattttgg cttttgttgt
     9481 aaatttaata gaatttgttt gctcatcatt tattcctgca atttttactc aagcactggc
     9541 tttacatgaa ctgcaaactt ggcaatatta tttttatatt ttgctgtatg atatattttt
     9601 tatgcttgat gatttgatta tcttcagttt tgcagttttt gctgtcaaca gaataagtac
     9661 acagtatgca agatggtgta agctcatcgg ggggatcacg ttatttgttt taggtattgt
     9721 tttactgaca acttctaact cgtaacctaa ttatcaaagg attttgctta tatttgttaa
     9781 gttatctatg aataactcaa ttcaaacagg atttattcct ataattcttt taatagaata
     9841 tccaggcaaa ttcaggttac aatcttttgt caatatttta atctattttc tattgcaaat
     9901 tcaaagaatt agattaattt atctaagatt aaagtgccac ttattggagg gtagcccctc
     9961 cttttgagta aagccaaact taatgagaga gccaactcaa tgccctcaac aagaaacatt
    10021 gaaacgcata acgactccaa tcccacattg cgagcactga gtctatctgc cttgggcata
    10081 gtttacggag atattggaac aagccctctt tatactttta aaacggttat attgctggcg
    10141 ggaggaggga ctccagacgt aactaccatt atgggctcag tatcattgat tatatggact
    10201 ctgataatca ttgcttcggt taaatacatc tcctttgctt tacggattga caacgatggg
    10261 gagggaggta ttctcgcgtt gatgtccctt ttaagtttaa aactaaaaca aaagcctttc
    10321 attattgcgg ttggacttat gggggctgct ctcatctatg gagatggcac aattacccca
    10381 gccatatcgg tgctctctgc tatagaggga cttgagatcc tttccccatc acttaagtat
    10441 tatgttttgc ctattgcgat aacaattcta ataacacttt ttgcgattca atctaaaggt
    10501 accgcaacta ttggaaaggc ctttggccca gtgatggcat tttggttttt aaccattggt
    10561 attttagggg caagaggggt tatccaacac ccttttgtct tagcggcaat aaaccctgtt
    10621 tatggcttaa attttttatt ttcaaatggc gcaacaggat tttttatttt atgtggggta
    10681 tttctttgtg tcactggtgc agaagcctta tacgcagacc tgggacattt tggtaccgcc
    10741 ccaattcgct gcgcctggtt tggtctggct tttccaagcc ttatctttaa ctatttggga
    10801 caagctgctt tggttttgga aggagcatcc acagagcata atatttttta catgctgtgc
    10861 ccaagtgact ttttattacc gttaataatt ctgtctaccg tcgcaacaat tatcgccagc
    10921 caagccatta ttaccggagc attttccatg acaagacaag caatgcaact gggctggcta
    10981 cccaggttac gagtcacgca aacgtcttca gagggttatg ggcaaattta tataggagtt
    11041 gttaactggt ttttaatgct ggctaccctt gggcttacta ttagatttgg cagttctgaa
    11101 aaattggctg ctgcctatgg cattgctgtt tcagccacta tgctttgcac cactgtgctt
    11161 ttatttattg ctctgcataa gctatggaaa tggaatatta tcacatcagg gcttgttgct
    11221 ggattattca tgattgtcga tgcctctttt tttgcggcta atctcacgaa atttatcaat
    11281 ggcggatata taccaatcac attggcaatc ataatctatt ccatgatgta tatttggcat
    11341 aaaggctata aaaccattgc gatcaagcaa aaagaaaaaa atataaccgt cgattccttt
    11401 ctggaaagta ttcaaaaaga gggggtagtc cgtgtgccta aaactgctgt ttttcttaca
    11461 tccaaagagc aggatattcc tccaatcctg gtttggcatg taaagaaaaa tcatgtgttg
    11521 caggataagg ttattatttt aaatattaac aacttgtcca ttccctggtg taaacctgct
    11581 gatcaattac aaattgttga aacaggagca ggcatctggc atgccgtagc caattatggt
    11641 tttatggaac aacctcatat tccaaaatta ttaaaaaaat tagaggctca aggctatgac
    11701 atcaatatca aagacataac ctattatatc ggtcatgaaa caatatttgt ccgcaatgtc
    11761 cgtcatacta tgtcaaaata cattaaaatt ctcttcgttt ttatgcatcg caatgccctt
    11821 cccatgagta attactttca tcttccgcca gaatcagtat ttgaaatagg acgacagata
    11881 gaaatttagt gaaaatgatt gctccctgac atggaaaatc tcagtaggcg agcacagttt
    11941 tatgatcagg attgaatttt gcacctgatc tcatagcaca tgggacaaat caaaatttaa
    12001 tcctggcttt tgctcttttg cagattatac ccaggtcctg aaaaagatta aaaacatacc
    12061 aaaaaagtgg cctataatta taaaacaaat actttggatg atagaaatgg ttcaatttgc
    12121 caaaccatct attggccctc cggctgattg tggacgaata aatctcttga ttggacgtga
    12181 gttagggcaa tatcaaaata tcattcaaaa atggattgct aatctcgaag acaacgcgaa
    12241 acaaaaacct tctaatattt ctgaatttaa atttggaaaa attttaggat atcttttact
    12301 tcaaagctta aagaaagcaa atttacaccc agacaacttc agatcttttg gttcagaccc
    12361 ttgggaagaa cctcattatc aatcggcgcg aatcgctgga aaatatgtgg taactcaaga
    12421 tacgtaccga atttactttg atataccaag aattgatcat tcagataatg aaaaaataaa
    12481 ggccaatcaa gaccatgcgc agatacttta tttcacaaca atgaccaatg atggccttat
    12541 ggttttttcc tttgaagaga aagtagagaa aatagagcct aaattatttg tggaactggc
    12601 aaaaaacata gaggtagaat caaatgcatg ggcacaatta attgacttca cagaaaaaaa
    12661 tttccacacc cgagaagatt tgaattccaa tccacaagtc cctcaaatat taggtctgat
    12721 tttatatgca actatctttc ccgaacacag gaacgaggct tttaatggcc tgtgcccatt
    12781 gctcttaaaa tcagagaacc ctaactttaa ctccattaca acaaaactgc ttaaagatat
    12841 tattcctgat tatgaagcca aagcgatggc attcctgcat aaaaacaaca atccaatgcg
    12901 ttatagcgaa agggacatta gtgagcaagg agctctttta ggtctttcag atgaaaaaat
    12961 tgacgtagta caaaacgaaa tgagagaacg taaagcatca gaagtacaaa acaacaatcg
    13021 atcacaagcc attgaactaa aaaaaactct cattggtgcc gtggatgaat accttagatg
    13081 gcgcaataat gaaagcaaag aaacggatta tcaaaaaagt tccggtgctt ttacctggct
    13141 gcgtcattac tccgattttg gcaaaaaccg agccaatgat ttaaaaaatg aacttaataa
    13201 agcacaagat ttaaagacca tgcttgatgt tttacaaaaa catttcgcaa ataactcccg
    13261 attacataat cattctcttg atagttacct gttaagagcc gtgtacaaag attttaataa
    13321 attcaattca atatttaatt ttgaacatct tacaattaaa aatgataccg atgctaaccg
    13381 tgaatggcta agagaagaaa tgttggggat ggcagcaaaa acgaacatga atgttacatt
    13441 ggaaaatccg acagttagta ggcaagaaag ccctactcta cccaataaag ctaaaatccg
    13501 tatcagtata atgaaaatac ctactaatga acgggaggaa aatattttgg cggtccacac
    13561 attattaaga aatgatgaat tgctgagaaa tcaagatgga tctgttccaa ctatcataaa
    13621 ggaaatacgg gacattgtag ggaaaatcga cccttcagaa gaagaaaaca tagcccatgc
    13681 gattattgaa atcaaaggaa aaatagctgg caacagcgac cataattaca acgagaatgc
    13741 gctcgacgtc atcaaagcgt ttgaaagtcc aagttgttgt gattttagaa aaatccgcgc
    13801 cgccttaacg accaaccatg caatcaatga aataatgaat ccggtaagag taggaatgca
    13861 gctaaactag ttcaaattct tttaccatag attatttttc tatctcataa ccatgcgaca
    13921 ataaagacga ctttgttcgg attaattaaa aatctggtta tagaagtaat gagcaaggtt
    13981 atttctatga attttctttt tgcttggcta tattgaaaca cccgtgattc gcaaaatgcg
    14041 caaaatactt ttaaatatta taatttcagc aatatcagtt gagcgttgga ccgccaacaa
    14101 gaggaaaagg acaaaaaaat aatggaggaa caacaacata tttcagctcc cggttggcat
    14161 gagcaatcag tagaaaaaac acttattatg ctaaacacta cagagcgagg gataagtgag
    14221 caggaatcca caaagcgatt agaatattat ggtttcaatc gcttgccaga accaaaaagg
    14281 aaaagtattt ttttacgctt cttgctccag ttccacaaca tactcatcta cgtattaata
    14341 ggtgcggctg tggtaacctc cttcctgcaa cactgggtag ataccgctgt tataatagct
    14401 gttgttatca tcaatgccct gataggtttt atccaggaag gaaaagcgga aaaagcgctg
    14461 gatgcgattc gaaaaatgct gtctcccgct gccacagtat tgcgcgacgg agagagacgg
    14521 catattagcg gtgagcggct tgtgccaggc gatattattt ttctggaggc aggagataaa
    14581 gttcctgctg acgtacgctt agtaagatct catggtttga gagttcagga agctatatta
    14641 acaggcgaat ccattcctgt tgaaaaacaa attcatcctg tcgcaaaaaa tacagaatta
    14701 ggcgacagga cttgtatggc ctttagtgga acattggtaa ccaatggcca aggcaaaggg
    14761 attgttgtag caacaggatc atccacccaa ataggacata ttagtggctt actatccaaa
    14821 atagagtcat taaccacacc attagtccta caaattgggc gatttgcaaa atggctcact
    14881 ttatttattc tgttgattgc cgcactgctt cttttttatg gttattttat ccaaaatcat
    14941 ccgtttgccg aattgtttat ggtcgtggtc agtttatcag ttgctgctat ccctgaaggt
    15001 cttcccgccg ttctttcaat cactctggcc attggagtac agacgatggc gcaacgacat
    15061 accattgtgc gccgattacc cgccatcgaa accttaggct ccgtatcggt tatctgtact
    15121 gataaaaccg gtaccctgac tatgaacgag atgattgtct cttctgtcct caccagtaat
    15181 catctattta ttttggatgg catcggttat gagcctcaag gaacaattac cttaaacgaa
    15241 caagtcattg ataacaatga atatcctttg cttaagaaac tcgcgcgtgc cgccatactt
    15301 tgtaacgatg ctgcacttca caaccaggaa ggaaactgga ctgtagaagg cgaccccatg
    15361 gagggcgcct tactggcatt tgccggtaaa acaggactgg ctatgcaaga agaaaacgtc
    15421 ttgtgggtac gtaccgatgt gattcctttc gatgccaaac atcggttcat ggccacctta
    15481 aaccacgatc atttgcagca tgcgatgatc tttgttaaag gtgcccctga gcaaattttg
    15541 aagatgtgcc aaaatcaaca aatcaatcag ggagaagtac agcctttaaa tgagccttat
    15601 tggaagcgac aaatggagca ggttgccgca agaggacaga gattactggc ctttgcggtt
    15661 aaaaaaacaa agccagaaca caccgttttg gaatttgccg atattaagga tggtttgaca
    15721 ttacttggaa tggttgggtt aattgatcct ccaagacctg aagccattga agctgtagca
    15781 caatgtcata cagcaggcat tcaggttaaa atgattacag gagatcatgc cagcacagca
    15841 ttagccattg gtcgccaaat aggacttaaa aacctggata aagtattaac aggcattgat
    15901 ttggagaata tggatgatgc gatacttaag aatgctgtgt tggagacaga catttttgcc
    15961 cgaaccagtc ctgagcataa actaagactg gtgatggcat tgcaatcgca cgggatgact
    16021 gtggccatga ccggtgatgg ggtgaatgac gctcctgcgt taaaacgagc agacgcagga
    16081 attgccatgg gcaaaaaagg cagcgaagta gccaaggaag ctgcggaatt tgtacttgtg
    16141 gatgataatt ttgcttctat tgtcgcagcc gttcgtgaag gtcgtactgt ttatgacaac
    16201 ctgaaaaaag tcatcagttg gactttacct accaatgcgg gtgagtcaat gaccgttatt
    16261 ttagctatac ttttgggact cagtttgcca gtttcaccaa ttcagatttt atggattaac
    16321 ttaattaccg cagtcacttt agggattgcg ctggcttttg aaccaacgga agaaagcacc
    16381 atgcatcgtc caccacgtcc tcgaaatgaa cctctgctaa ccggcagttt agtatggcat
    16441 attattctgg tttccatttt attcatttgc gggatttttg gactctattt ctatgcactc
    16501 gatcgcggtt actcgattga gctggctcgc acaatcgctt tgaatactct agtggtactt
    16561 gaaatttttc atctgtttta tattcgtaat atttatagta cctcactcac ttggaaagca
    16621 gttcagggga caaaagtagt ttggatagca atcatagtgg ttactgtagc acagtttgca
    16681 atcacctatt tcccgccttt gcaaacaata tttgttaccg aaagtgttcc atttggggat
    16741 ggagtattaa tcgttctaac tggagctaca ttttttgctg tgattgaaat tgaaaagcag
    16801 cttcgtttga gattaagggt tataagccct ccaagtgcaa gagaaaaatg ctcattataa
    16861 tgataaactc ctgtgatgga ttggttataa atccatcgtc taattctgac aatcaagtcc
    16921 tctctggtta ggcaatgact aagaaagtac acatttccat gattttaagg tatggttatt
    16981 cactcatcta atgtgaaaaa catacattac aagggttgtg aacaatgacc atctattttt
    17041 tgtttggcaa aactggctcg ggtaaaagtt atattggaga gctgctcaga cagcttaaca
    17101 tcatccatat cgatggtgat aaccacataa cccaaaaaat gcgtgactgt ttaatcgaag
    17161 atgaacaaat gactcctgag atgattgatg aatttgtcga tgtcttgata gatgttatta
    17221 atactcaaaa agaaaaaaca cccaaagaga gttttgctat ttcgcaagcg atgtatctcg
    17281 acaagcaccg tatcaagtta ttaaatgcca tacccgaact caaatttgta atgattgatg
    17341 tcacgcctgt actgcgggca agtcgcatca ccaaccgatt tcgcaaccag gaaagcaaag
    17401 taagcttgcg ctacgccacc aaaatggata cattttttga gtatccttca catgaaacaa
    17461 tccggcttga aaacaatcaa ggctcagatg aaacgcttat agagcaaatc cgtgaaaaat
    17521 tgccagcact gtttaacatg gactcaaaag aagaattaaa cccaggcata aagcttcagt
    17581 ttgcgtaata ttcagatcca tatccagata tggttgaaat aaagaactta tgtagcaact
    17641 gtcttgaagt taggacttaa gacagttgcc acttgccagt tgggattaga gaatttgacc
    17701 ttttaaaaca gatattgtaa accaagagcg acagtatagt gtttattgga tagccctgct
    17761 gaatcaccga aataactgcg ctcacccgtg tcattgtcaa tgatttcagt atccgctctc
    17821 ccattagaat aatgattgta agaagcctca acaaaaattt tcgtattagg tttcaaaaag
    17881 taggcagaat gaatcgttgc cgcataatat ctggagctat ttccccgctc attgaatgtt
    17941 aagttgcggg cataatgctc atcatgatct cgtgctgaaa cccaatcact aaactttaaa
    18001 agaccactaa actcaaaatt attaataaaa tacttccctg ccagaccaat ataaggagtt
    18061 ctaaattttt gttggtatcc tattcctggt tgatcactgg gaaaacaacc ggcatagagc
    18121 cctccgttat attgataaca gcctccagta gcttgccagt caaaggaatt ccattgataa
    18181 ccagtggcaa gacctaactt gtaattttgt ttttgcatca accaggccct taagctcata
    18241 tctaactcat tcgcataatt gagatacgta tcctcatgat gagaccagtg agtccaggtt
    18301 tcttgataag gattgaacca atcataatca tccatagcag ccttattttt taccaaggtt
    18361 gtccaacctc gaccattcat gctaagccaa tccaggacat cataatttaa ttcccctttt
    18421 attatcggag cattttttat gcgccaatcc agctggctta acttggtgtc tgtctctgga
    18481 tggtaaacat attcatgggc gttacctgac agaatgccta gagaagcact taatgacaaa
    18541 ccattaaatt cgtaatccgc agcataagta ggaaatgaga aaagaaagga taaagagact
    18601 ggagccaaca aaactatttt atttttcata ttttttccta gttaaggctg gcaatccttg
    18661 cagcaaagtg actttaaaaa accaatcgca ttattgtgat ccataatgat cttgtcaaga
    18721 tcaacattga ataatattga tcactaagga ctctaatcaa gcaatatcat atcgctcaat
    18781 tgagaaagca ggagcagtta tattagtgtg tgtattttgg gcaatcatgt tttgagtgta
    18841 ggcactgggc tttctggaca atagggcaaa accaaataca agcaaactga aaactaataa
    18901 atacaacaaa tcccaatact ggggaaggat atgcttacca ccataattac caagaaatga
    18961 gaaaatacca agtccaaaaa gatagaatat aaaccatagt gcttgtttgc tatcaatctc
    19021 ccgatgtgta tttttcataa gacaatagag gaaagaacac actactccca gaatagtgat
    19081 caccaataac tttcgtacgc tatggaaacc tgcccaataa acaccgactg tacaaacata
    19141 aaatccgaga aagccaataa ggttactccc ctgcaaacga aaaggacgct tatagtgcgg
    19201 taattgctga cgtaagcaaa ccagtgctac aggccctatg gaatagctaa tcattaatat
    19261 tgccacaaga aaagccgaca tttcttgcca tccgtgcaac acgagcgaca tggtggcagc
    19321 gagtaagaaa tttacgccta aactaaccca gggaacttga tacctgtttt gtttggtaag
    19381 aattacaggt gcaggaacaa ctgagcccat actgcttagc atgtgagctg ctgtggttga
    19441 ataaacaaga ccagtggaat aaggtgatat aaaagcatcg gcgtacaata aaatgctcaa
    19501 ccaaagcatg ccagccagtg cagcgagagc agcaaatggt cctgcatcac cagtaaaaga
    19561 taaattcacc caaccttttt ccagcgcttg ttcgctaaca ctcccaataa aggaccattg
    19621 taaaccagtg taaagtaacg tcacgaacac tagtgaacca cccaaaatca gaggtatgta
    19681 ttgtcctggt ttctccaact cacccatcat gataaccact tgtcgaaacc ctaaaagtga
    19741 aaaaagcaca cctccgtttg acattgctgc cataacgccc tgccagccat aaggcataaa
    19801 accaccgtgc tgaaaaaaat tacccgggtg ataactcatg gttaccaagc ttgcgatagt
    19861 aagaataggg attattatct tccagatagt aaatgcggca ttaatgcggg caaacaaacc
    19921 aataccaaaa taattaaaca aaacaaaact cattaataca accagggaca gcatataacc
    19981 tgctggcgta tttcgatagt gcaaagcgtc atgggatacc agatctggaa tgtaattact
    20041 ggcatattga atgacacctt gcgtttcaat cactggcaaa atggctaggg aaaaccaggt
    20101 catgacattc atgataatgc cagtcaaacg tccatgggta tataaaggca agcaagccag
    20161 gctgtcagac tgtggaaaca tcgtacctaa ctcagcgtaa gacaaggcaa caaacagaag
    20221 taaaaaacct gccagtggcc aagcgataat ggcagcagga ccagcaaaat gagcagaata
    20281 taaagaacca aataaccagc ctgagcctac catgctggta attgaaatcc aaagtaaact
    20341 caaagcactt aaatttttat tcatctgatt ttcacatgtc cttatgattt tgatgcaggc
    20401 aaacaattca ccagtcagca gagcgaaatc attatatttt tgcattgtaa acagaaatat
    20461 catgactacc aaccctataa gagtcaagtt taaccaggta ggttacgaat accttccagc
    20521 atcaggaaaa atgaaaaaca agaactagca gcagtgattt tctttgctac gaattcggca
    20581 tttttcactc tgtaatcttt cgcttgacag tatctctgct tcgattgctt agctgccctc
    20641 ccttaagcta tagttaggtc tctagtgaat tatgatctgc gaccaggagt gtctatggat
    20701 ggaaaagttg ggaatacagc aaccgggtgt ccggtgatgc atggcgggat gacatctgca
    20761 gaaacatcca acacagcatg gtggcccaat gccttgaacc tggatatatt gcatcagcat
    20821 gacaccaaga caaatccaat gggaaaagat ttcaactatc gtgaagaagt caagaaactg
    20881 gattttgagg cgctcaaaaa ggatttgcac gctttgatga ctgacagcca gccatggtgg
    20941 cctgctgatt gggggcacta cggtggttta atgattcgta tgtcctggca tgctgcaggt
    21001 tcttatcgtg ttgcggatgg ccgcggcggt gctggtacag gcaatcaacg ctttgctcca
    21061 ttgaattcct ggcctgacaa tgtgaacctg gataaagcac gtcgcttatt atggcccatc
    21121 aagaaaaaat atggcaacaa gattagttgg gcagacttga ttgtcctggc tggcaccatc
    21181 gcttatgagt caatgggact aaaaactttc ggttttggtt ttggccgcga agacatttgg
    21241 catcccgaaa aagatgttta ttggggttca gaacaagaat ggttaggcgc caagcgttac
    21301 gatggcaaaa gccgtgaatc tctggaaaac ccgctggcag cagtacaaat gggattaatt
    21361 tatgtaaacc ctgaaggagt gaatggtcaa cctgacccgc tgcgcactgc ccaggatatt
    21421 cgtgtaacct tcggacgcat ggctatgaac gatgaggaaa ccgttgctct gactgccgga
    21481 ggacatactg ttggcaaatg tcatggtaat ggcaatgcaa aatttttggg ccccgatccg
    21541 gaagccgctg atgttgagga ccaaggcctt ggctggatta acaaaaccac cagaggcatc
    21601 ggccgtaata ctgtttcaag cggtattgaa ggcgcatgga cgactcaccc tacccagtgg
    21661 gacaatggct acttctattt attgctgaat tacgattggg aactgaaaaa aagccccgcc
    21721 ggtgcctggc aatgggagcc catccatatt aaggaagaag ataaaccagt agatgttgaa
    21781 gaccctgcca tccggcacaa tccgatcatg actgatgctg acatggcaat caaaatggat
    21841 cctgtttatc gtaaaatcgc cgagcgcttt tacaaagatc ccgactattt tgcagaagtt
    21901 tttgcacgag cctggtttaa actgactcat cgcgatatgg ggccgaagac gcgctacatt
    21961 ggtcctgatg ttcctaaaga agatttgatt tggcaagatc cggtgcctgc tggtaacagg
    22021 gcatatgata ttgctgccgc caaagcaaaa attgcggcca gcaatctgac catcggtgaa
    22081 atggtttcta ctgcatggga cagcgcgcgc acttttcgcg gctcagataa gcgcggtggt
    22141 gcgaacggag cacgtatacg actaaagcca caaaaagact gggaaggcaa tgagccacaa
    22201 cgcctgacta aagtcttgcg aattcttgaa ggcatcgctg ctgatacagg cgcaagcgtg
    22261 gcagatgtga ttgtacttgc cggaaatgtc ggtatcgaga aggctgccaa ggcagctggc
    22321 tttgacatta tcgttccctt tgctcctggc cgtggggatg caaccgatga catgacggat
    22381 gcagaatcct ttgacgttct ggaaccactt catgatggct atcgcaactg gcttaagaag
    22441 gcatatgatg tccgtcctga agagctcatg ctagatcgca ctcagctcat ggggctgaca
    22501 gcccatgaga tgacagtcct tgttggaggt ctgcgcgtgt tgggtactaa tcacaacaat
    22561 acccaacatg gtgtattcac tgatcgcgtt ggtgccctga ctaatgactt ttttgtcaac
    22621 ttgaccgata tggccaatgt atggatacca agcaaagaca atttgtacga aattcgagac
    22681 cgtaaaacgg gtaacatcaa atggacagcg actcgtctag atctggtttt tggctctaat
    22741 tcgattctgc gctcctatgc tgaagtctat gcccaggatg ataacaagga aaaatttgta
    22801 aaggactttg ttgctgcatg gacaaagatt atgaacgccg atcgttttga cttggcataa
    22861 tcttttgcaa gttgacatgc cacagataat catagtagaa atagtttcga ttatgtttag
    22921 ctttgattgc cttttatcgc atcaaaacta aacatagcgt actaccataa aatgttgttt
    22981 caaaagcaaa cttttacgat ggtttgcatt aggcctgatt tgccaaactt gtctcttttg
    23041 ttttatttct gcgttgcaaa cgtttctgta agtctcatct actgcatgta aactccaccc
    23101 ttcaaaacgt ttgcgccttg ccctaaaaca aaataatcag atcatgcaag aggtctattt
    23161 atattatgtc tcaccacaaa cgacatgctc cagcatttat attactggaa gatgcttcat
    23221 cattgccttt atcttgtgcc ataaccgaat gcatttcaga cgatattcca ggttgtgctg
    23281 attgaaagaa accaaatgct tctttagcct ttacatttaa actgcgtgaa tcacttaaaa
    23341 tttcactaca aaccaagctc tttttttcta ccatctttgc atcaagagcg ccctctgcaa
    23401 taatggatgc gcgcgcttgt attttaaatg caagctccac tcgcattaaa gcatcttctt
    23461 caatactcac attacttgac aaacgtctta tttcttcatg actcggaact tcttttctct
    23521 gtgtactgga atgacaaatt acctgaggat aattttcttc cagtattttt tgcatattaa
    23581 ggcccattgc aataccacaa ccctcttggc taaattgttt cgcttgacaa aaattgattt
    23641 tatcaatggc tggatagtga aatttagcca ataacgcatt gacctgtgga aggatatgca
    23701 ttttgtaatc ttccatgtaa tacatataag tgtagtaatc aaatgaaact aataagtttt
    23761 ctgattcaat ataataaagt gaaggagtat tattttcatc aatagataac atacacaaat
    23821 tccagtgatg aggaccacct tttgtagcca ctgggattag tagttcccta actacagaac
    23881 tgttatttaa tgaacacatt tcctggacat atggcagaag aattttactt atatcgccat
    23941 tgtttttttg attaaattta taatccttat ctccagcagt ttcaggcaga tgaataaaag
    24001 gggtgattgt ctgcaaacgg gttgagttac tcttggaatg aaaattgtta agaaccatat
    24061 gcattaacaa attgacatca cggatattaa aagtttcatc catatccaga atattattat
    24121 cttgaaaaat tatgggattt ccatcaattt ttactttaat tcttcggtgt ctgttttcac
    24181 cttcttcaac ttcaggtatt tctatttcaa ttcttttgct aaatggatta ccgtccagat
    24241 caatatcttc tttatagtag gtgataattt ttgattgttt ttcataataa tcgagcggtc
    24301 tctctgaagc agatccccaa ggaatagggt agtgagattc ggtaataatt tctgtatcaa
    24361 taccgcctat ctcttttttt tccacataaa tatattggct ggatttttga tctgcatttt
    24421 gatcatttgt tctcttatta tttttcattt atttctcttg gaaaatagta atatttatta
    24481 cttcaataca ttcctctcaa ctattccctg atacttggca aaaaatttct gaatactttg
    24541 atctcaaaat taattaccca tgagaaaaca ctgaagtgcc tttaacttct tttatctggt
    24601 ggatttgaaa tttttaaaaa cagagggcta tctgatatag cgagattttt tcgcacctta
    24661 gagattgatt tcgtatatca gggttgatac cactcttttt cagcattcgt tggtccgcag
    24721 taacttgctt ttaccgagct gaaaaactgt tttttgttat gtattaaatt gcaaatcctt
    24781 ttcaggtttt gcatttcttc cggagaaata aaaactggtg cagtatctgg caaaccaatt
    24841 ggcttgagaa tccacaattt tggaaactat ttttttcata aatagaaatt ttttataagg
    24901 tgagaccaac ttccgtatgt ccgcagtcag gataaatata ttttacacaa ccaaactggg
    24961 cacatttttg tgttagatca ctgtttttcg taccataaac taataaatta attcgtgcct
    25021 ttttcttgaa tcaaccatag ggcaggaagg gtcaacaaag ccatcaccat caaataaata
    25081 gaaaatgcaa aatggatgaa aacataactt aatagcaaaa taatgagtgg tgctgtgcct
    25141 ccaaataggg aattagacaa gttaaaaaat aaagtaaagc cactggcgcg ctcttcctgc
    25201 ggtaattgtt caaggctggc ggtaaatgcg cttccaataa aaaaagagat aatgacagcc
    25261 aaaacactgc aagctacagt aatgtgtaat aaagtacctg tattgatcaa ataatttatt
    25321 accaaaacgg tacataaagt tgcaattaaa ctgactgcca aaggaaagcg cctgcctaaa
    25381 atatcagagg tgaaacctga taataagaga cccatgctat aacaaatcat ccctatgata
    25441 atgacatcca tcgctgtagt taatggcaac tcctgctgtt gcagatatga aggcatataa
    25501 acaaaaaatg tataaaagct actgttgcaa taaattgcca aaagaaaaat agcagcaatt
    25561 gcttttttcc gataacgcaa ggcattcagt atgggatgag attgtctttg aacaattact
    25621 tttgcaaaat cggttaattc tctggtttga cttcgcaaat aaagtccgat aaaacctacg
    25681 aatattccta ttaaaaatgg gaagcgccag agaaaatcgg gagactggct ggaaaaaaat
    25741 atcagggtta ttttgcttac tattattgca agcaacaatc cacaaataga acctaaagtg
    25801 acgaaactga ctgctaaacc tttatgacgc atgttcacat tttcataaat gtaaatactg
    25861 gctagtgtca tttcccctgc tacagaaacc ccttgagtta atcgacatag cgtgagtaat
    25921 atcggagcga gaatgcccgc ctcttgataa gcaggtaata aaccaataaa acttgatgaa
    25981 aaagtcatga ccataagagt catagtcaat gtttttttgc ggccaatttt gtcacccaga
    26041 ttccctaacc aataagcaga gaatggtcga acaagaaaac ctgcggcaaa aataccaaat
    26101 gttgcgatga gagcagtgtg tttatcagtg gcaggaaaaa aaagaggcgc caaaatgggc
    26161 gccataaacc cataaatggc atagtcgtac atttcaaaca aggtgccaaa agaggctgcc
    26221 gcaataattt ttttatccat gttcaagctg ttgattttgt ttcacaggta gtaatacagc
    26281 ttactttaac tctattgatt tctgagcttg attctctgaa tccacttcat ccttgactgg
    26341 tttaggacga ccaaaaaaac cggctatact aggccttgat ggtaacacag agacaatcaa
    26401 ttgtcccaat gaaactgttt cggactcaag aggacttggt aattcctcaa gcgagagttt
    26461 ttgcgggtga ttggcgatat agtcattact gtcttcgaga tatttacaaa taaatgattc
    26521 ataaacccca agacctgtat catactttag caaatacaaa tcggccagat gcgtgtccaa
    26581 ttgaccgtca ctgtttctgc catatttcgt ctcgagaaat cttattcctt gttcgcttaa
    26641 tgaggcccaa tgccactgtt tatagatatc tcttttaata cgatactctt caggacaggg
    26701 atcaacgaaa tgctcaagtt gagaattgga ttcaacgctt tctgcaagcg ttgcatgccc
    26761 tgcccctaag gtaagatcac ctgtatgatt gatatgaatt tggtaagggg tatccaggcc
    26821 atagttatcc ccagggcgaa tatcccaacc agtatagcga agcagagggc gaactgtata
    26881 acgcaaagta gagaatgcac ccagatttcc ttcgacggca gcttgaaaag tggaaaaggt
    26941 attattcaga atgcatctta ctgttacatt tgattgcagt ttgaattgat cactgacttt
    27001 ttttgccacc gcagcaccaa aggagtcgcc ttgaaaaagg atatcatcaa ttgatatgcc
    27061 tttttcaagt aaggtattgg ccagcgctat tccagtgtta accatatcat tgacttctaa
    27121 cacttcacca cccagttttc tcatgcccgg gtattcaaaa gcgtaataat gcgctcccgt
    27181 tatttgaaca gcttttgcta tatcaatcag gcaatcttga tattgtgttc ctattccagt
    27241 gaaataaatc acatgttttc cctttccaac agctttgacc ttctcattta gtaaggagct
    27301 tgattgattg gaattattct gaaaccctat cacctcggtc atacaggttt tttggttggc
    27361 taaagtcacc tcgatttcaa agcgatgaaa gtctatacct gataattgtt catggtaggg
    27421 agaatcctct tgacgtattg cctctacaaa acaggcaaaa cgttcttcaa gtgacatctc
    27481 tgaattaccg aaaccatttt tgacatacca gtcctctcta ttcggaggaa aagttcttaa
    27541 agctatttgt ctttgcaacc agatttgtat acttgaattc ggcatagcac tccccttttt
    27601 ttgatcaata agtgtgcata caatttgcta atgagtcaac atttctttgc gcatctttat
    27661 tagagataat tcttattttc aacacagcac cgatttgcac ccgattgcag caaaacaaac
    27721 tcactttaac cctgcgctta taagaaattt gtataggcaa catacaaaca attgatttta
    27781 taaatgaaca gtcggttgat attattccct atttgattaa aacaaaatct atttatttgg
    27841 gagattttca ccatgtttta cgaattaata ttaaccagaa ccagcaacct gatacaagaa
    27901 tttatctcta tccctaatgg cgttacttct cttgacttga gtttgaatga attaggcaat
    27961 ataagtaatg ctgaattaac tcaggccttt cagtatatcc ctgacagcgt tatatcgatg
    28021 gatttggcta acaatcactt atgcgacaaa agcggctcag aattggcaca actcctggcc
    28081 gccatccctg ccaatgtaac ttctctcaat ttaagctgca atgatctcca caagaaaagt
    28141 ggtgctgagt tagcacaagc ctttgctgct atcccagcta gtgtaacttc gcttaatttg
    28201 cattgtaatt acctgggcaa taaccgtggg gtggagttag caaaggcctt tgcggccatc
    28261 cccgaaaatg tgacctcact ggatttaagt atgaattatt tcgatctgga aagtagtgct
    28321 gacttatcac aaatttttac cagtatccct cctcacgtgg cgtcacttaa tttgagcttt
    28381 aactctttgc atgaagtacc ctttgaaaag ctagtactcc ttaaggattc gctaaagcat
    28441 gtgcaaactg tttatttgag cttttatagt gtcaaagaaa tgtccaaaga acaacgaaga
    28501 gcactgggcg ctgcatttcc caatgcgcaa aaaatcattt tgattgatga ttatggccat
    28561 gaaattcaac cgtctataat aatttctaac ctgattaggg aactttctgg aaaggcagat
    28621 gctccatcct tgttaaatca atgtattctt tttacccaaa gacatcaaaa agacagtgat
    28681 aacaagatca tccctaagga attagaagag agcattcgaa cttttaactc tcactgattg
    28741 ataatcttga gcaaccgtcg ttcgctcttg tcaatgaatt aaaaatacca aaaacttgtt
    28801 tgactcttcc gagtcaaaca agaaatggaa taatcgacaa agaaaaaaaa ctcttgttaa
    28861 tctttgaaag ttgtcgggga attaagcatt ttggatattt ctccaagatg catttcttct
    28921 tcttgaatca tttgtcttgc aaactcttca actaacaccg acttattttc tgaatattgt
    28981 aatagttgat aatacaaatc cagactttgc ctttcatgct caagcgactc ttctaaaata
    29041 ttgctgatat tatgctcatg agtctctaat aacttaccga ttttaagtgt aggatgatag
    29101 gaaaaatggg taataagctc accgattaga tgagcgtggt tcaaactttc ttgagcctga
    29161 tcgcgcatcc atttgcagat cgggatgcga ttaaatccaa agaccataaa ggagtaatga
    29221 gtatagcgca caacacccgc tagctcgatt tccaataatt tatttagagt ttttatgatg
    29281 gcatcatcaa tgtcttgagt ttcagatctt ctatgtttca taggattacc tgtcatttta
    29341 cttatctaag tatagaataa tttcaagaaa atctcgaagc gttatcagtt attgagaatt
    29401 gcctctcata tctaacataa gccctgat
//
LOCUS       DASLOE010000024        29020 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.024, whole genome shotgun sequence.
ACCESSION   DASLOE010000024 DASLOE010000000
VERSION     DASLOE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 29020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29020
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.024"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(23..1504)
                     /locus_tag="U6309_000830"
     CDS             complement(23..1504)
                     /locus_tag="U6309_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN5504283.1"
                     /translation="MNIAIIGGGTGLTTAWALENQRKFHVTVFEENDRLGGHIHSIKI
                     NNVILEGGAEFIGPPALYPNVHRLFQSLGMALDQFELNMDFDNLKQRDHIVLPPLYHT
                     SSGKSEDVSSILYTLSCGLFGKRRKQNETRVSVDTMLTEMYNLLEMNHLIMDARNKLL
                     HPDNLMTLEQFINEFKEKCVHVLSPVDKFAEEFVYPLIASGWGVSIETIKKFGAHYAM
                     NYLAAGMNWFDAPEGLSSYIDKMVAQCTNTQFKVNTAIKKLIPITIDGKIKYQLLKRD
                     DTFVCDATGSPMIYDDVVISTPAYVTNELLSDIESNTIKVLREKLANVTYYDTTVVFH
                     QDPEYLSPYRTVVHSRFDGTQSANTMCKPWKFNKDEIPIMKTWVLPGQEMPKNVLREV
                     HYKHPVMDKNYYEAQQALHAAQGEAGLWHGGILAGFNDSHESGITAALQVAAQLNHRE
                     RCLNKNERLALFPNIVDSVLSPKLQADMGGEVEGLASSSMSYA"
     gene            complement(1709..2620)
                     /locus_tag="U6309_000831"
     CDS             complement(1709..2620)
                     /locus_tag="U6309_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504284.1"
                     /translation="MYSKYPVFFPNKNIKSFSGVRFSNVVGIPSAIESLYRGDNNLAG
                     IIFLLPTLITGVFCQNFPEVVDMEQVRLHKLTNLSNDFHMVSMSEDPQIALDWGKGCF
                     ITIDPVLFSDYIVDVHATFRKNQLNFPDRMEQEREHIALAVPFCSIKKITIDNKELAN
                     PFYLSIPQDNHEARMEFNTLYSELISLLRKKYTQEVDGEEEKVALRAYAIRYLDFYSK
                     FSGCNNPFNKSLAQLVELYPEFMSNFLQSSHLSSKTGLMKDIVLNSSDNLFKEHPYTQ
                     SIDASYIHRVKESTTCYEDDWAKPVYD"
     gene            2829..3671
                     /gene="htpX"
                     /locus_tag="U6309_000832"
     CDS             2829..3671
                     /gene="htpX"
                     /locus_tag="U6309_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEN5504285.1"
                     /translation="MINNLKTFILLASLTALLVVIGGLLGGSTGMLVALLFAGIMNFS
                     AYWYSDILVLKMYNAEPLSNNHFVYHIVAELAHRAGTSVPKVYLINNSTPNAFATGRN
                     PENASIAVTTGLLDRLTQEEITGVLAHELAHVIHRDTLINVVSATIAGTISGIANMFM
                     WLSMFGHSSNNEEGVHPVVGMIMMIVAPLAAGLIQMAISRSREFEADAGGARISGNPQ
                     WLASALLKLDQANHEQYFDEAETHPATAHLFIINPLNGEKLANLFSTHPSTAERVARL
                     RAMY"
     gene            complement(3797..4771)
                     /locus_tag="U6309_000833"
     CDS             complement(3797..4771)
                     /locus_tag="U6309_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5504286.1"
                     /translation="MDKNSKSFGEANERIGNAIHHVCLNASDAKFIKSNIQESYQNWA
                     NVLYDRVFLNWGLCDKKIYRELSALDFDFSKVGFFPHVYGQSLLFYLIRPLIKIHFFN
                     KRLLDVGCGNGVGLKISSELLKTRYALGIDLVNKLVTNSNNNFYIEDTINYMQADAEN
                     MAIANESFDIVTNLESSHLYPQIEHFFSEVERVLAPNGFFCYADVNFDVKRQAERFET
                     FVKTRKNLRIIEKHNITKMIQASIYQRLIVNEKGFYQLAQYLLGPDKIHYTSELANVA
                     SSMGLMFLPWWRIRFKNPSLQALGKFARRCKHWNKKYYFYYLIQKIDS"
     gene            complement(4929..6161)
                     /locus_tag="U6309_000834"
     CDS             complement(4929..6161)
                     /locus_tag="U6309_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEN5504287.1"
                     /translation="MNRPVVIVDPLSSGIELAPAFKARGIPAIAVTLKPLDWIGFGAN
                     MQTSDFIEIIPDQPNLVEILAKYDPIAIIPGTEEGVPLAEALAINLTPQFANDPEKSQ
                     NRLHKALMQKALQEAGVPALKTLNTASENEVEAWIRTNELIDSPLIIKPPVSAGSDKV
                     FHIPARGEWKKAFNQVLSEPSKITGKNNETVVVQEQAIGTEFAVGTVSADGKHYLAHL
                     IQYNKTSFNDRKTVYDYVEFVPYSEEIYGELFDYTKKALDALGIRWGAAHNEIMLTKG
                     GPRLIETGARMCGGPVVGFAREATGSSQADKLVEIYVDGGVSTEEYVFKKTVVPVFLK
                     SPAKGKISNVEAFADLSKLPTFLNEHIWFKNGDLVPQTVDYLTSIGIIGLAGDRKSIL
                     LDYEKIRNMESKLVIQTF"
     gene            complement(6158..7306)
                     /locus_tag="U6309_000835"
     CDS             complement(6158..7306)
                     /locus_tag="U6309_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5504288.1"
                     /translation="MKKSLVFLAIGMFTVGCNTFLIAGLLPQIGETLGQPVAVTGQGV
                     SLFSLTYLLSAPLFSLIFANQPVKRMVQLALTVFMFGNLITLLSENIVLFLIGRSLAG
                     AGTGIFTPLCISIAVHFASPSTKGRILSFIWSANSAGVVFGVPAGLYLSSLFHWQLSI
                     ASLIILSLLALVGFSMQNIDIRLPKPSPFGGRLRLLVEPKTLSVIGITCFTALASLGL
                     YSYVTLIQSGSPNSLSMTLLSWGLGGFIGSSLIGVFIDRTGKPRVIMALILVGLMFAL
                     IAIPFTRNLPYLGLIPFFMWGACGWAIVTPQQHILYELHENQGTILAAINSSALGLGS
                     ALGTLLGGLLISSGFRGIYLPFSAATLLFFVLIIQLIVINTSHKVNNI"
     gene            complement(7303..8745)
                     /locus_tag="U6309_000836"
     CDS             complement(7303..8745)
                     /locus_tag="U6309_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEN5504289.1"
                     /translation="MSAQVADVVIVGAGPVGLMCAYLGQLCGIHTVIVDKSDGPLEVG
                     RADALNARTLQLLELVDLFDELYPLGKTCNTSSVWADGQFISRQSSWWEELEGCLHKH
                     FLMLGQSYVEKLLDEKLKETAATVKRSTAIVNIEINEAGCLTTLANGERIQSRYVIGA
                     DGARSFVRNHFAIPFEIIRPQIIWAVIDGMIDTDFPKVPEIIVFQAETSDVAWIPREG
                     EIDRFYVRMDTKDFTLQEAMDKINHAVRPHSLNFKDIVWFSQFSVKESVAESFFIQDR
                     IFLAGDACHIHSVNGGQGLNTGLADAFNLMWKLHMVMHFGAPKELLRSYEAERKPVAH
                     GVITTSGELVRSTKYSLNGTHAQDYVRIVQKRAGYITGMGIRYGETGLCGSRLFDFEI
                     FNGIAKTRLYSLLDYMKFTLLIFGDCEVELQPPEFVKVISIYPTQCQENFWTDNTHYA
                     NQAILVRPDSYIQCAVPLDKMESLFELAQL"
     gene            complement(8742..9578)
                     /locus_tag="U6309_000837"
     CDS             complement(8742..9578)
                     /locus_tag="U6309_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEN5504290.1"
                     /translation="MNYKVTSLKPFGVLLEPVSEKTKVTDVDIENLRHLFAKNQLVVL
                     RGFCTFQNAEDFSNYCELWGEVSLWPFGKVLELIEQENPEDHIFDHSYMPLHWDGMYR
                     PHVPEYQIFHCVKAPLPGQGGRTTFSNTILALQFASSEIKELWNKVSGTYQRKMEFYN
                     SKTVSPIITKHPQKDFSVIRYNEPPSADKGHFVNPPDIEFTGIDQEELDFFHRSLNKA
                     LYSADNFYAHEWQNGDIVIADNFSLLHGREGFVSRSPRHIQRVHVLSNPPFDNPGLES
                     YQ"
     gene            complement(9568..10617)
                     /locus_tag="U6309_000838"
     CDS             complement(9568..10617)
                     /locus_tag="U6309_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocyanide synthase family protein"
                     /protein_id="HEN5504291.1"
                     /translation="MKNTAFLITENHYPKDYRLKKSPDSDSAMGVAKRILAEVMIFRR
                     VPETISLCDTGCQKCASPHLPKIISAVNKNEPVTFILPAFPGKSPNPEKVLGHLPDHA
                     ERLSLNFLGTLCQRIKNFYTPGIKIILCSDGRVFSDVVGMNESDVTAYQLELDRLIKE
                     MSLTDLSTFNLDYFYKDLHFVQMRDELMKSYGQSLDFLKQKIRDGAKPSASPDEQEAN
                     RMYSGITRFLFEDAMHAGQTKSRTAIQKESRVKAYEVIRRSNAWSALIAERFPNAVRL
                     SIHPQTCGSKKLGIRLIGNENWMTPWHGVAVESNKGYILLRRSEAEALGAKLICSSDG
                     RRSHYQLMAEVSYEL"
     gene            10728..11588
                     /locus_tag="U6309_000839"
     CDS             10728..11588
                     /locus_tag="U6309_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN5504292.1"
                     /translation="MSLLLDDIRYFIAASDTLNMTRASEIIGISQPALSYSIKRLENK
                     LGGLLFIRLKNGIQLTKLGEEFKRRAHRLLYEWEQVQNIANPESGFVQGNYTIALHPS
                     VALYTLQYFMPKLQVNYPGLGINFIHGHSREMTEKVISWEADFGIVVNPIQHPDLVII
                     KLSTDEVTVFYANDAQNKLIYDRKLAQSQYILKKIGKKVTFNGVINSTSLEVVAKLTA
                     LGLGYGILPTRVTYPYKHLKKLLNAPMFRDEICLVYRPEKHNNPVSKKIIQIIRSSIS
                     NDNDKENYFD"
     gene            complement(11718..12443)
                     /locus_tag="U6309_000840"
     CDS             complement(11718..12443)
                     /locus_tag="U6309_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504293.1"
                     /translation="MLYNKRGYLMTTLYIKNLRYNEVAVCRALFEAAEKTEEGIKTKR
                     YGIQMTANMPGLWSPLSNYDIENDLRMARIQRNGRVREIGYLTVNLVFQFDSLDVTEY
                     ETKYGISAKDVLEKAGFTCSQDAAGYSEPVSTLTRLLGQESKPEKPTQTSVVNTSFFS
                     LKSVDTDKRFTKSTKPGILYEARSYGKIEDDTLKSYGFTDGQWERQKEGIENIVIIKK
                     LTQSISPSPDILGEAVDNIPTLS"
     gene            12633..13199
                     /locus_tag="U6309_000841"
     CDS             12633..13199
                     /locus_tag="U6309_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-box protein"
                     /protein_id="HEN5504294.1"
                     /translation="MKAKYDPTEPGLQKLPPEIKVMILKFLDAKSKLALSQSNYGWRD
                     LIRHLPECTKEITNTLFRLDKKRHHQEIAQMMSGRVTASSMAKLFEELLCFSIPSSYV
                     FLIFFASQKSVALVEVLTVILVFAAITSLAHDLVDYFIESDTKAEKQHAHRRAFQFFA
                     QPSQSAVQQNSEEENLSADPKAWPCEPL"
     gene            13465..14220
                     /locus_tag="U6309_000842"
     CDS             13465..14220
                     /locus_tag="U6309_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN5504295.1"
                     /translation="MYVFRRFSKHIITFLHSLELAFRFLGHLVHSTIKVIFGKLSIVW
                     PNTLEMIYYSGARLVILLSFIGILLGATVSQTVYALLKPFQLHQRVLPIVQNILTHEI
                     LPVLIGFILCIQAALHMVNTRLEHYQENPEAIILAQVLPIIVGMNIASLLLYVYLVSA
                     IFFSFYLTFHFMLGFTNNEFLSYVVSSTTLFDLIYSVVKTLILCIIVGLTSGYYYYEA
                     SIRHIYLRKAVSRILTRGSFWLIIASMYITLTF"
     gene            14288..14974
                     /locus_tag="U6309_000843"
     CDS             14288..14974
                     /locus_tag="U6309_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEN5504296.1"
                     /translation="MILGGTFFYIEYKRAQKQTFVMFFKGSLKGLVTTAPVTYRGVKI
                     GEVKVIEITENKEHSKVLIPVYVQFFVERTYGFSQDPIHLLIDNGYVANITKPNLLTG
                     VAEIELIKPTPAVKYKQTYYHSYPVFPTHNSAEKYTSMEEAFEAAKKAFEDVSELVRS
                     KEIQDTLEAIQKVSENLGQLASSLNQDVPSVVAYLNQSLKQITSAAYSTQNLTDYLSR
                     YPESLLRGKR"
     gene            14971..15681
                     /locus_tag="U6309_000844"
     CDS             14971..15681
                     /locus_tag="U6309_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEN5504297.1"
                     /translation="MKNQKHFLKFYCIRDRSWPASARASFEGIRFLSFSNYAEFRKKP
                     VKLLLLLLIVLTLCDCGRSKETQFYLLTPISPQKNSHNSYNHLQIGVDEVSIPDYMKK
                     PQLMIHQTPHHLTLEEFNQWAGALDKNITLVLTTNLSTLMPGVVVQTSPWNNKFHPDY
                     HLQVMISQFEIDIHGNSILRAEYLIYRKEELIYKGNAYYHIKIPLISIETLVQSMNTN
                     LTHLTEEIARFFIKNTTK"
     gene            15782..16192
                     /locus_tag="U6309_000845"
     CDS             15782..16192
                     /locus_tag="U6309_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEN5504298.1"
                     /translation="MIGKDKWEILTEKMTSLHIYEADLSEKFILGSGKGGQKLHKTAS
                     TVYLKHLPTGMEIKCQESRSREDNRYFARQRLCEKLQAVLSDEKTKTQQRIEKIKRQK
                     RRRSRRAKQKMLDEKSRQSQLKTLRKNPRSHDIE"
     gene            16459..17343
                     /locus_tag="U6309_000846"
     CDS             16459..17343
                     /locus_tag="U6309_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HEN5504299.1"
                     /translation="MAGTSLLTLIDDIATALDDIAVMTKVATKKTAGVLGDDLALNAE
                     QIIGIHANRELPVIGAVAKGSAVNKLILIPLAILINFFTPALVGILLILGGLYLCFEG
                     FEKIVEKIFHKKNEPAKKKIDTQTIDMRQFEKEKISGAVRTDFVLSAEIIVITLGTVA
                     ATSLINQIAVLTLISLIMTIGVYGLVACIVKLDDLGFILREKKHPLKQIGILLIGAAP
                     ILMKILSVAGTIAMFLVGGGIITHQITAIHHFTENLSAMMSIGVDFFIGIISGAITAA
                     LYFTFKKMKSKFKLMNTK"
     gene            17512..17967
                     /locus_tag="U6309_000847"
     CDS             17512..17967
                     /locus_tag="U6309_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN5504300.1"
                     /translation="MPDIKHYLKPMVLFSGLLVAPFAMAEATKAEETSTVKLSTILQQ
                     LSDQGYKNIHDAEIDDGVLTVEGSNEQGVKFEIHLDPATGKILKKQEHPQPKYSIIDI
                     TKKIEDEGKFKIIEIEFDEGIYKIKGETDSHEKKELHIDPNTGETKTDH"
     gene            complement(18049..18822)
                     /locus_tag="U6309_000848"
     CDS             complement(18049..18822)
                     /locus_tag="U6309_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN5504301.1"
                     /translation="MYKTVHFYSNNLKLEGKLEEPTGKCLGYVLFAHCFTCGKDIAAA
                     SRIASALVSNGFAVLRFDFTGLGSSEGSFSETNFSSNVEDLVAAADYLRAHYRAPVLL
                     IGHSLGGAAVLLAAKNISEVKAIATIGAPASAYHVKHHFSADLSKIESDGEAQVTLGP
                     RSFTIKKQFLQDIDRYQDTINSDAGKALLILHSPIDKVVSIKEAEKIYKAAKHPKSFI
                     SLDKADHLLSDKRDSQYAAEVIAAWASRYLTPSSEDQSG"
     gene            19247..19666
                     /locus_tag="U6309_000849"
     CDS             19247..19666
                     /locus_tag="U6309_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEN5504302.1"
                     /translation="MKTYRKELWFNIPERMGFINITDKVIECLKESSIQEGLILVNAM
                     HITASVFINDDESGLHQDYKKWLEQIAPHEPIRQYRHNDTGEDNADAHIKRQIMGREV
                     VVAITEGRLDFGPWEQIFYGEFDGRRDKRVLVKIIGQ"
     gene            complement(19795..20772)
                     /locus_tag="U6309_000850"
     CDS             complement(19795..20772)
                     /locus_tag="U6309_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504303.1"
                     /translation="MNLKKEVFLNQNCAEMMIKKAAQLILGSDLDFEYTRGVQDIQVD
                     LGPAFMFSPDEEKTLWVSGKNQETLEKDLATLNKSSVYFFRTGTQGGAGHWQVLYYEA
                     AKSGWVSYSSQSNHFQVTDSNGKLTASGKGLLVPHANWGKENGNYVFLLVNASAQNII
                     HAANFVYILRTQNEVAAIEYCALNHEFHPEIKRTARAKAEPETRTKGEIHNPHKDLSC
                     ENLKTIYSKHVALLHVINRLEAGSKSYNPYWMGSQAKLNTILGALEACYQKGLDVDNE
                     LSNSNSELSKAINIRRLPSIFSCWGGKNESPVESRKIINENRTYRRNDP"
     gene            21008..21487
                     /locus_tag="U6309_000851"
     CDS             21008..21487
                     /locus_tag="U6309_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504304.1"
                     /translation="MKEKTAKQLKLFIIYLLSYWLLSIISCLIAFGYDDSLRMLLASP
                     KSDLSGALLFFSSFIATALLFVFRYKTFSDKPYPYFIFGFYVGNVSLLMLFILDAFIR
                     ELIVWKFPEFLLVFISPFVELVLSYLFFGFAFLAIIPAVTSAFILYAVQKKLLLPPI"
     gene            21662..21874
                     /locus_tag="U6309_000852"
     CDS             21662..21874
                     /locus_tag="U6309_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParD-like family protein"
                     /protein_id="HEN5504305.1"
                     /translation="MGIVKISDELHEATKLMARAMSRSINSQAEYWIRIGKLAEENPS
                     ITYTDILKILVHQASLGEIYDVSEDA"
     gene            21855..22616
                     /gene="map"
                     /locus_tag="U6309_000853"
     CDS             21855..22616
                     /gene="map"
                     /locus_tag="U6309_000853"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN5504306.1"
                     /translation="MLVKTPEEIKKMRVAGHLAASVLEMIEPYVIAGASTRELEQVCR
                     QYIIEDLKAIPSTLNHYGFPACICTSINHVVCHGIPSEKKLKNGDIINIDVTVQKDGY
                     IGDTSKMFLVGNIKPFAKKLVEVTQECLYKAISIVRPGAHLGDIGNIIQTHAEQHGYS
                     VVREFGGHGIGKSMWEDPHIMHFGKPNTGLRLQAGMTFTIEPMLNLGRKEVKTLGDGW
                     TVVTKDHKLSAQWEHTILVTDNGHEILTLRADEHL"
     gene            22721..23803
                     /locus_tag="U6309_000854"
     CDS             22721..23803
                     /locus_tag="U6309_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEN5504307.1"
                     /translation="MPCEKCDSAATYHLSGTYQLLILPPRGHCYSKLLKHLTENNFDI
                     NKRDSGIIELLFTAERAQDIGHALERCYSQAELEDSKAVLLPIDIADRNIERVLNHTH
                     SLKKIVGLCLSQWLVGLIENQGLSTFCQPILHNTTLKPYGFECLLRGKQDNNILPSSD
                     LFGAAKSSDMLFLLDKKARICHIENMSKIDIADKKIFINFNPTAIYDPLFCLTTTNQS
                     LKKTSIKPAQIVFEVTESDEVKDKKHLLKIIDYYRNQGYCVALDDLGSGYSSLNLLTE
                     LQPDYVKLDQELISQIHTNEFKQIILEKICEMATKLKIKIICEGIETEDELQIIKNYA
                     IDFYQGFLFERPMPYEKIPDYIKASA"
     gene            23833..24711
                     /locus_tag="U6309_000855"
     CDS             23833..24711
                     /locus_tag="U6309_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEN5504308.1"
                     /translation="MLTNIERAKLQLIDETSDMVIITKAEPLDAPNGPEVVWVNKAFE
                     NISKYKANEIIGQTPRILQGKETSQNTLKMIKEAMKAQTNINVDLLNYTKDGTPYWIN
                     FSIIYLRDGQGNLCYLGAIEKDITAIKNLNIQLSEKAKTDTLTNVYNQENFFIAGNEA
                     VKNFHQNQETVGLLFFDVDDFKGINDTQGHIYGDKILKKVAQESLELVRKTDNVFRYG
                     GDEFAIIFSGINVKILERKAQQLQKKLAKSHISVSIVWTISKQSDKSINTLLARADEA
                     LYYVKANKKGLFFIRE"
     gene            complement(25059..25805)
                     /locus_tag="U6309_000856"
     CDS             complement(25059..25805)
                     /locus_tag="U6309_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504309.1"
                     /translation="MKRYIWFHPSYVLESAEATLLGQQTNAKDHGYILLNDQELGKVT
                     CEDHLTIMGHSTLAPTPAEDTGLYIQGDTADDLIRRLTTNGLKEAPKILSLESCRAGI
                     SGGLAQQLSRSPFFKYTLIEANLNSVGRKPMEMQWNFPTDSLGRAVLKLDNSPWMFYL
                     GGFLVGRLTHDTYTTEDLFSLLSPPTFHKHFFANYQPGFFGGRSGRHCLFNKTTITLE
                     KASNFAHEDQDSASAKALEVVLKKLYSQPA"
     gene            complement(26066..27502)
                     /locus_tag="U6309_000857"
     CDS             complement(26066..27502)
                     /locus_tag="U6309_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504310.1"
                     /translation="MTPLFPQKGQPVAIKQGGSGSCFLLACLDCLLNGEAEGVDVVRK
                     KFKQTTSGVTPRIKRTDNTMKVQWEKMAGKYTYSYDPRTDEDVIFIDNEKLKEIDNSK
                     KGSSSNSLAVKILEHISAYYYQIAWDGESLLAHKLHPKFRNNPIAKQSDTAFLAKLLG
                     VHVTSFYDPRKLVKLKTIDPMIPVYVGMNFGKLDRLGRHRRHALRIGNIKPLDDGEYE
                     VTLINPWNNQKQTTCLLTALRKRKCHFAVFDSSPQKKLLTEIFLEYKTEIGCELFRNK
                     ELAFLLCEMKQCGLLDSELAKYSLSSCMVLYKKLPNLVANYRCLPYSERYNMLMCVAS
                     SNGHVRLFNKLMQGSSVVDKPSVARSPQRPYPSAKSFFADLFVSLEASLDRVHYNDLL
                     KTLRVNYGHLRSVNTALNQVENRQGIYSKMELRNMNQRLERLGFFMKCHTDPRGHRHP
                     DLGQQPNFCDRSTQKVNVKSHQDKGISL"
     gene            complement(28073..28426)
                     /locus_tag="U6309_000858"
     CDS             complement(28073..28426)
                     /locus_tag="U6309_000858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504311.1"
                     /translation="MIKIYNNCTLKNQLSHGLKGQFTVIKGQCELIQTRVDTGAAPIR
                     LVVWCFYGRASRIVVVYGKQFQSFFAPLMMILISEICSNPPWSPVFKKHTLSMPSIVM
                     NKLVVSGLIATFGGQ"
     gene            28494..28718
                     /locus_tag="U6309_000859"
     CDS             28494..28718
                     /locus_tag="U6309_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504312.1"
                     /translation="MKPIKNSKEQEHIAPEAQDPRLSYTEDDRRPLTSTTSAKHPSRM
                     FGKSEERSARESYDQYGNNVTTYWTQGPWN"
BASE COUNT         9097 a         5836 c         5328 g         8759 t
ORIGIN      
        1 taataaatat agtgaaaatt ctttaagcat atgacattga agaagaagcc aatccttcaa
       61 cctccccccc catatccgct tgcaatttag gagatagaac agagtcaact atgttaggga
      121 atagtgcaag acgttcattt ttatttaaac aacgttctcg gtgattcaat tgcgctgcaa
      181 cttgtagtgc tgctgtaatg ccactttcat gtgaatcatt aaatccagca agaataccgc
      241 catgccataa cccggcctca ccttgtgctg catgcaaggc ttgctgtgct tcgtagtaat
      301 ttttatccat gaccgggtgt ttgtaatgaa cttctctcag tacgttttta ggcatttctt
      361 gcccaggcaa aacccaggtt ttcattatag gtatttcatc tttattaaat ttccagggtt
      421 tacacatggt gtttgctgat tgagtcccat caaaacggct gtgcacaact gttctgtagg
      481 gagacaggta ttcgggatcc tgatgaaata ctactgtcgt atcataataa gtgacattcg
      541 caagtttttc ccgtagcact tttatcgtgt tactttcaat atcagacagc agttcattcg
      601 taacataagc cggtgtagag attacaacat catcataaat cattgggctt ccagtggcat
      661 cacaaacgaa tgtgtcatct cttttcaata attgatactt tatttttcca tcaattgtta
      721 ttggtattaa tttttttata gccgtattca ctttaaattg agtgttggtg cattgcgcca
      781 ccattttatc aatatagctg ctcaaacctt ctggcgcatc aaaccaattc attcctgctg
      841 ccagatagtt catggcataa tgggcgccaa attttttgat tgtttcaatt gaaactcccc
      901 atcctgaggc aatcaaagga tacacgaact cctcagcaaa cttgtcaaca ggcgagagga
      961 catgaacaca tttctcttta aactcattta taaattgttc taatgtcatt aaattgtctg
     1021 gatgcagtaa cttattcctc gcatccataa ttaagtggtt catttcaagc aaattgtaca
     1081 tttctgtgag catggtatcc actgaaacgc gagtttcatt ttgtttcctt ctcttgccaa
     1141 acaaaccaca agacaatgtg tatagaatcg acgaaacgtc ctcggacttt ccactggagg
     1201 tatgatacaa aggcggtaaa acaatatgat ccctttgttt tagattgtca aaatccatat
     1261 ttaactcaaa ttgatctaaa gccatcccca gagattgaaa caatcgatgc acattgggat
     1321 acaaggcagg tggtcctata aactctgcac ccccttcaag gataacattg ttaatcttga
     1381 tactgtgaat atgcccgcct aatcgatcat tttcctcaaa tacagtcaca tgaaattttc
     1441 gttgattttc caaagcccaa gcagtagtta atcctgttcc accaccaatt atcgcaatat
     1501 tcattacttt tttagtcctt ttaaaagtcc atttccaaat gccaggctcg taaactaaat
     1561 ggttagaata aaaaagtaaa gtgtctttct gtggttaaat ttataaaccc ggagcctacc
     1621 cttcttggcg catcggtctt aatgtactcc tgtcaaaaac tttttccata tatgtacatt
     1681 aactccattt tttttattac tttgttggct aatcataaac tggttttgcc cagtcatctt
     1741 cataacaggt agtgctctct ttaactctat ggatgtaaga agcatcaatc gattgagtat
     1801 aaggatgttc tttaaataaa ttatctgatg agttcagaac aatatctttc atcagacctg
     1861 tttttgatga aagatgactt gattgcaaaa aattgctcat aaattccgga tagagttcaa
     1921 ccaactgagc aaggctttta ttaaatggat tgttacaacc gctgaattta gaataaaagt
     1981 ctaaataacg tatggcgtaa gcacgtaatg cgactttctc ttcttcccca tcgacttcct
     2041 gagtgtattt ttttcttaaa agtgaaatta attcgctgta gagcgtatta aactccattc
     2101 ttgcttcatg attatcttgg ggtatgctga gataaaaagg gttagctaat tctttattgt
     2161 caattgtaat tttttttatg ctgcaaaaag gtacagctaa agctatgtgc tctctttctt
     2221 gctccattcg gtcaggaaaa ttgagctgat ttttacgaaa agtagcatga acatctacaa
     2281 tataatcaga aaataaaaca gggtcgattg taataaaaca tcctttcccc caatccagag
     2341 caatttgagg atcctctgac atactcacca tatgaaaatc attagatagg ttagttaatt
     2401 tgtgcaatct tacttgctcc atatccacaa cctcaggaaa attttgacaa aacactcctg
     2461 taattagagt aggtaaaaga aaaattatgc cagcaagatt attatcacct cgatataatg
     2521 attcaatggc agagggaatt cctacaacat ttgaaaaccg cacacctgag aaagacttta
     2581 tattcttatt tgggaaaaat actggatatt tactatacat aagatgttta atgacatata
     2641 aatttgcaca acaacatacc acattgatct caaaataaaa ataaccgcgt attacgatca
     2701 atgggttttc ttgatctcct actctaaagc atggacataa acagagataa cgctccaagt
     2761 tgaattttta acatctggcc ttatatatga gatataaaat acaggaagag tcaatgaggt
     2821 gatgagaaat gataaataac ctgaaaacgt ttatcttgct agcctctcta acagcattgc
     2881 tagttgttat tgggggattg cttggcggtt ctacaggaat gctggttgcg ttactattcg
     2941 caggaattat gaatttttca gcttactggt attcagatat tcttgttttg aaaatgtata
     3001 atgctgagcc tctttctaat aatcactttg tttaccatat tgttgcagag cttgctcata
     3061 gagcaggaac ctcagtaccc aaagtttatt taattaataa ctctactccg aatgcgtttg
     3121 caacagggag aaatccagag aatgccagta tcgctgttac aacaggattg ttagataggc
     3181 tcactcaaga agaaatcacc ggagttttag ctcatgaact ggcccatgta atccaccgcg
     3241 acactttaat taatgtggta agcgcaacaa tagcagggac gattagcggg attgccaata
     3301 tgttcatgtg gctttcaatg tttggccaca gttcaaataa tgaagaaggt gtccacccag
     3361 tggttggcat gatcatgatg atagtagctc ccctggcggc tggactaatt caaatggcta
     3421 tatcgcgctc cagggagttt gaagcggatg ccggtggagc acgaatttcc gggaatccgc
     3481 agtggctggc cagtgccttg ttaaagcttg atcaagccaa ccatgagcaa tactttgatg
     3541 aagcagaaac tcaccctgcc actgcgcacc tgtttatcat taatccatta aatggagaaa
     3601 agctggcgaa cctcttttca actcatccct caacagcaga gcgggtagca aggctaagag
     3661 ccatgtacta attgaggttt ctctcattga ttcggaataa gcatcgcttt aatcacaacg
     3721 ataaacatag tactctatct cagttatact cagtgcatct caataaaacc gtgcactgga
     3781 aatagtggat tggtttttat gagtcaatct tttgaattaa ataataaaaa taatattttt
     3841 tattccagtg cttgcaccgg cgagcaaatt ttcccagcgc ttgcaatgac ggatttttaa
     3901 agcgaatcct ccaccaggga agaaacatga gccccattga actggccaca ttagccagct
     3961 ccgaggtata gtgaatctta tcaggcccca aaaggtattg agccaattgg taaaaccctt
     4021 tttcattgac aattaaacgc tgatagattg atgcctggat cattttagta atattgtgct
     4081 tttcaatgat gcgtaaattt tttcttgtct tcacaaaagt ttcgaatcgt tccgcttgcc
     4141 gtttcacatc aaaattaaca tccgcataac aaaaaaaacc attaggcgct aaaaccctct
     4201 ctacttcaga aaaaaaatgc tcgatttgag gataaagatg tgaactttca aggttagtga
     4261 caatatcaaa actttcgttt gctatagcca tattttctgc atcggcctgc atataattaa
     4321 ttgtatcttc tatatagaag ttattattgg aattagtgac caatttgttg accaaatcga
     4381 tgcctaacgc gtatctggtt tttaataatt cagaacttat tttcaaacct acgccattac
     4441 cacaacccac atcaagtaat cttttattaa agaaatggat ttttattaat ggccttatca
     4501 aataaaaaag aagtgattgc ccataaacat gagggaaaaa acctacttta gaaaaatcaa
     4561 aatccaatgc cgacaattcg cgatatattt ttttatcaca taacccccag ttcaaaaaaa
     4621 ctctgtcata aagcacatta gcccaattct ggtagctctc ttgaatattg gatttaatga
     4681 attttgcatc cgatgcattt aaacagacat gatgtatcgc attacctatt cgctcgtttg
     4741 cctcaccaaa gcttttggaa tttttatcca ttttttattt ttaaaataaa taatataagt
     4801 ataggccgat ttataatgga taagcgcttt gtttgttagg ctaaagaatt gttcatctat
     4861 aactcaagtg actataatac ttcaaaccat tgaaaaaata tttcaatggc agttcagcaa
     4921 tagaaaccct aaaatgtttg gataaccaat ttcgactcca tatttcgaat tttttcgtaa
     4981 tccaataaaa tggacttgcg gtctccagcc aacccaataa ttccaatact cgttaaataa
     5041 tcaactgttt gtggaacaag gtcaccattt ttaaaccaaa tatgttcatt aagaaaagtc
     5101 ggtaatttcg acagatcggc gaaagcttct acattcgaaa ttttaccttt agcgggtgat
     5161 ttcaaaaata caggcactac cgttttttta aatacatatt cttctgttga aacaccccca
     5221 tcgacataga tttccactaa tttatcggcc tgactactgc cggtcgcttc tcgagcaaag
     5281 ccaaccactg gaccaccaca cattctagca cctgtttcta tgaggcgagg tccgccctta
     5341 gtgagcatga tttcattgtg ggcagccccc cagcgaattc ccaaagcgtc caatgccttt
     5401 ttcgtataat caaataattc accatatatt tcttcgctat aaggcacaaa ttccacatag
     5461 tcgtaaaccg ttttgcgatc attaaaggat gttttattat attggattaa atgcgctaaa
     5521 tagtgttttc catcggcact caccgtacct acagcaaatt cagttcctat agcctgctct
     5581 tgcacaacaa cggtttcatt gtttttcccg gtaattttag agggctccga taaaacctgg
     5641 ttgaatgctt ttttccactc ccctctggct ggaatatgaa agaccttatc actgccagca
     5701 gatactgggg gtttaataat aagtggagaa tcaattaatt catttgtcct gatccaggct
     5761 tcaacctcat tttcagatgc cgtattgagc gtcttaaggg caggaacccc agcttcctgc
     5821 agcgcttttt gcatcagagc cttgtgtaat ctattctgtg atttttcagg atcattggca
     5881 aattgaggcg tcaagtttat cgccaacgct tccgcgagag ggactccttc ctcagtacca
     5941 ggaataatgg caatagggtc atattttgca agtatctcca caagatttgg ttggtctgga
     6001 ataatctcga taaaatccga ggtttgcatg tttgctccaa atccaatcca atccagaggt
     6061 ttaagcgtga ctgcaatagc aggtatccct cgagccttaa aggcaggagc caactctatt
     6121 cctgatgata atggatcgac aatgacaaca ggcctgttca tatgttattt accttatgtg
     6181 aagtgttaat tactatcagc tgaattatca atacgaaaaa caataaagtg gcagcagaaa
     6241 aaggaagata gattcccctg aatccagagg aaatcaataa gccgccaagc aacgttccca
     6301 aagctgaccc caagcccaag gccgatgaat tgatagcggc aaggatagtt ccctgatttt
     6361 catgtaattc atataaaatg tgttgctggg gagtcactat agcccatccg caagcacccc
     6421 acataaaaaa agggattaat cccaggtaag gcagattcct ggtgaatggt atggcaatca
     6481 gagcaaacat gaggccaacc aaaattaagg ccataataac ccgtggttta cctgttcgat
     6541 cgataaatac cccaatcagt gagcttccta taaatcctcc cagtccccaa ctcaatagcg
     6601 tcatactgag cgaattaggg gatcctgatt gaatcagggt gacatacgag tatagtccca
     6661 aacttgccaa ggcggtaaaa caagtaattc caattaccga tagcgttttt ggctcgacca
     6721 gaagacgaag cctgcctcca aatggcgaag gtttgggtag tcttatgtca atgttttgca
     6781 ttgaaaaacc aaccaatgca agcaaactta aaataataag actggcaatc gataactgcc
     6841 aatgaaacaa ggaggataag taaagtccag caggaacgcc aaacactaca cctgcactgt
     6901 tagcactcca gataaaactt aaaattcgtc ctttggtaga tgggctggca aaatgaacgg
     6961 caatgctgat acataacggc gtaaaaatcc cggttcctgc tcccgccaga gatcttccaa
     7021 ttaaaaacag cacgatattt tcagaaagta gtgttattaa attgccaaac atgaagacag
     7081 taagcgcaag ctgaaccata cgctttaccg gctgattagc aaaaatcaga gaaaaaagtg
     7141 gcgctgagag aagataagtc aaactgaata gactcactcc ttgcccagtt actgcaaccg
     7201 gctgcccgag cgtttcgcct atttgaggaa gcaaaccagc aatcagaaat gtattgcaac
     7261 ctactgtaaa catacctatt gctaaaaaaa ccaggctttt cttcataact gtgccaattc
     7321 aaataaggat tccattttat ccagcggcac agcgcattgg atgtaggagt caggccttac
     7381 taaaatggcc tggtttgcat agtgtgtgtt atcggtccaa aaattttcct ggcattgggt
     7441 tggatatatt gaaatgactt tgacaaactc cggaggctgt aactcgactt cacaatcacc
     7501 aaagataagt aaagtgaatt tcatataatc cagaagagag taaagccgtg tcttggcaat
     7561 gccattaaaa atttcaaaat caaagagtcg agaaccacac aaacctgttt ctccataacg
     7621 aatccccatc cccgtgatat agcccgcgcg tttttgcaca atcctgacat agtcttgggc
     7681 atgggttcca ttaagcgaat acttggtcga gcgcacaagc tcaccggaag tcgttatcac
     7741 accgtgagct actggtttac gctcagcttc ataactgcgc aaaagttctt taggagcacc
     7801 gaaatgcatg accatgtgca atttccacat gagattaaat gcatctgcaa gaccagtgtt
     7861 aagaccttgc ccaccgttta ccgaatgaat atggcaggcg tcaccggcaa gaaaaatgcg
     7921 atcctgaatg aagaaactct cagcgacaga ttctttaact gaaaattgtg aaaaccagac
     7981 aatgtcctta aaatttaagg aatggggccg cacggcatga ttaattttgt ccattgcttc
     8041 ctgcaaggta aagtctttag tatccattct tacgtaaaac ctgtcgattt ccccttccct
     8101 tggaatccag gccacatctg aagtttctgc ctgaaacacg atgatttcag gaacttttgg
     8161 aaagtcggtg tcaatcatgc catcgatgac cgcccatata atttgtggcc gtatgatctc
     8221 aaaaggtatt gcaaagtggt tgcgaacaaa ggaacgagcc ccatcggctc cgatgacgta
     8281 gcgtgactga atacgctctc cattggcaag agtcgtaaga catcctgctt cattaatctc
     8341 aatgttgaca atcgctgttg aacgttttac tgttgccgca gtttccttca gcttctcatc
     8401 taacaatttt tctacatagg attgaccaag cataaggaaa tgtttatgca aacaaccctc
     8461 caattcctcc caccatgacg attgtcggga aatgaattgt ccatctgccc aaacagaact
     8521 ggtattgcag gtctttccta aaggataaag ttcatcaaat aaatcaacta attcgagaag
     8581 ttgtaaagtg cgcgcgttaa gagcatccgc tctaccaacc tctaaaggac catcagactt
     8641 atcgacaatc acggtatgga taccacaaag ttgtcccaaa taggcacaca tgagccctac
     8701 tggacccgca ccgacaatta caacgtctgc aacttgtgcg ctcattgata agactccaga
     8761 cccgggttat caaaaggcgg attactcaaa acatgaacgc gttggatatg gcgaggcgac
     8821 ctggagacaa acccttctct gccatgtaat aaagaaaaat tatctgcaat gacgatatct
     8881 ccgttttgcc actcatgagc atagaaatta tccgctgaat aaagcgcttt atttaaactt
     8941 cggtggaaga aatccaactc ttcctgatca ataccggtaa attcaatatc gggtggattt
     9001 acaaaatgac ctttatctgc agagggaggt tcattgtagc gaatgactga aaaatccttt
     9061 tgaggatgct tcgtgataat tggtgatacc gtcttgctgt tataaaactc cattttcctt
     9121 tggtaggtac cactcacctt attccataat tctttgattt ctgaagaggc aaactgtaac
     9181 gctaagattg tgtttgaaaa agtagtcctt cctccttgtc caggcaaagg cgctttcaca
     9241 caatgaaaaa tctgatattc aggtacgtgg ggccgataca taccatccca atgcaagggc
     9301 atgtaactat gatcaaaaat gtgatcctcc gggttttctt gctcgattaa ctcaagtacc
     9361 ttgccaaatg gccaaagact gacttctccc cacaactcgc aataattaga aaaatcctcc
     9421 gcattttgaa aggtgcaaaa gcctcttaat acaacgagtt gattctttgc aaataaatgg
     9481 cgcaagttct ctatatcgac atcagtcacc ttcgtttttt cgcttacagg ctccaagagt
     9541 actccaaatg gttttagaga agtcacttta taattcatat gacacctccg ccattaattg
     9601 ataatgactg cgccgaccat cagaggaaca aattaatttt gcacccaaag cctcagcttc
     9661 cgatcgcctc agcaagatat accccttatt gctttcaaca gcaacaccat gccaaggagt
     9721 catccagttt tcattcccta tcaaccgaat ccctaatttt tttgatccgc aagtttgtgg
     9781 atgaatagac agccgaactg cattgggaaa gcgttcggca atgagcgcac tccaggcgtt
     9841 gcttcttctt atgacctcat aggctttaac acgagattct ttttggatag cagtacgact
     9901 tttggtttga cctgcatgca tggcatcttc aaacaaaaaa cgcgtaattc cgctatacat
     9961 acgattggct tcttgttcat cggggcttgc tgagggtttt gcaccatcac ggatcttttg
    10021 tttaaggaag tccagggact gtccatagct tttcatgagt tcgtcacgca tttggacaaa
    10081 atgaagatct ttataaaaat aatcaagatt aaaagtggac agatctgtaa gggacatttc
    10141 ttttataagc ctgtccagct caagctggta ggctgtgaca tcactttcat tcattccaac
    10201 cacatcgcta aaaacccttc catcggaaca cagaataatt ttaattcccg gtgtataaaa
    10261 attttttatt cgctgacaaa gtgttcccaa aaaattaaga gaaagacgtt cagcatgatc
    10321 tggcaggtga cccagaactt tttcagggtt gggagatttt cccgggaagg ccggcaatat
    10381 aaaagtaacc ggttcatttt tattaactgc cgagatgatt tttggcaaat ggggtgaagc
    10441 gcacttttgg catccggtgt cgcataagct aatggtttcc gggacacggc gaaaaatcat
    10501 tacttcagct aatattcttt tggctacccc catagcagaa tcactatcgg gtgatttttt
    10561 aagtcgataa tctttagggt aatgattctc tgtaattaaa aacgctgtat tcttcatttt
    10621 ccttccctcc tgaaaaagag agtacagatc ttctacaaat aattaaaatg atttatttta
    10681 attgttgtga taaattttac ttatacctaa agtggagttg atgagttatg tctttattgt
    10741 tggatgatat aaggtatttc atagcggcca gcgatacgct caatatgaca agagcttctg
    10801 aaattattgg aatatctcaa cctgcgctta gttattctat aaagagattg gagaacaaac
    10861 ttggtggatt actatttatc aggcttaaaa atggaatcca gcttacaaaa cttggagagg
    10921 agtttaagcg gcgcgctcac cgtctattat atgaatggga gcaggtgcaa aacatagcaa
    10981 accctgaatc aggttttgtt cagggaaact atactatagc ccttcaccct tccgttgcgc
    11041 tttacactct ccagtacttt atgccaaaac tgcaagtgaa ctatcctggg cttggcatta
    11101 atttcattca tggtcattca agagagatga ctgaaaaagt gataagttgg gaagccgatt
    11161 ttggtattgt tgtgaacccc atacaacacc ctgatctcgt tataataaag ctgtctacgg
    11221 atgaagtgac agtcttttat gccaatgatg cacaaaacaa actgatttat gatagaaaac
    11281 ttgcccaatc acaatacatc cttaaaaaaa ttggcaaaaa agtaactttc aatggcgtaa
    11341 ttaattctac cagtcttgaa gttgtagcca aactcacagc cttaggtctt gggtatggca
    11401 ttctacctac gagggtcacc tatccctaca aacaccttaa aaaattactc aatgcaccga
    11461 tgtttagaga tgagatttgt cttgtctacc gacctgaaaa acataataat cctgtcagta
    11521 aaaaaatcat tcaaattatc agatcatcca taagcaatga taatgataaa gaaaattatt
    11581 ttgattaatt cagctattac aatgattttt gcttattttt tgaggtaata gaatttattg
    11641 caaaaaatat aaaataccga aattgattga taatagacca ttgtttttca atttcagtat
    11701 tttaaatttg atgcccttta ggatagtgtt ggaatattat caacagcttc acccaaaatg
    11761 tctggacttg gagaaatact ctgagtgagt tttttaataa tcactatatt ctcgatgcct
    11821 tctttctgcc tttcccattg tccgtcggta aaaccatagc ttttcaaagt atcatcttct
    11881 attttaccgt aggatcttgc ctcgtataat attcctggct ttgtcgactt ggtaaatctt
    11941 ttatctgtat ccacactctt tagagaaaaa aatgaggtat taacaacact tgtttgggtt
    12001 ggtttttccg gtttgctttc ttggcctaat aaccttgtta aagttgaaac aggctcagaa
    12061 taacccgcag catcttgact gcatgtaaag ccagcttttt ctaaaacatc ttttgctgaa
    12121 atgccatatt ttgtttcata ctctgtaaca tcgaggctat caaattgaaa tacaagatta
    12181 actgtcaaat atccaatttc cctcactctt ccattgcgtt gaattcttgc cattctgaga
    12241 tcattctcaa tgtcataatt agaaagagga gaccataaac caggcatatt agccgtcatt
    12301 tgtatgccat atctcttggt ctttattcct tcttcagtct tttcagcagc ttcaaataag
    12361 gcacgacaga ctgctacttc attatatctt aaatttttta tgtaaagcgt tgtcattaga
    12421 taaccccttt tattgtaaag caggcatttt atccattttt gctcaaaata aaagcgtgtt
    12481 taccgattgg acttaatgaa aacgcattaa ttactcccgc atatcaatat tttatatagc
    12541 ccctatacaa ataaatcatt tttgacacaa tgcaaaacta cctaaaataa attttaattt
    12601 tttcagaaaa taaataattt aggttattca ctatgaaagc aaaatacgac cccacagagc
    12661 ccgggctcca aaaattacct cctgaaatca aggtaatgat tcttaagttt cttgatgcca
    12721 aatcaaaact tgctctttca caatcaaatt atggttggcg tgatttaatt cgacatctgc
    12781 cagaatgtac caaagaaata acgaatacat tatttcgtct tgataaaaaa cgccatcatc
    12841 aagagatagc acaaatgatg tcaggaagag ttacagcaag ttctatggct aagctatttg
    12901 aagaattact atgttttagc ataccttcgt cctatgtgtt tttaatcttc ttcgcatcgc
    12961 aaaaatctgt agcgcttgta gaagtcttaa ctgtaatcct tgtgtttgct gcaatcactt
    13021 ctcttgccca tgatctggtg gattatttta ttgaaagtga cacaaaagct gagaaacagc
    13081 atgcacatcg ccgtgctttt caattctttg cccaacccag tcaaagtgct gtacaacaaa
    13141 actcggagga agagaattta agtgctgatc ccaaggcctg gccatgcgag ccattgtagt
    13201 tcgtgccttg gcccgacatt tatcgaatcc atctacttta ttcttcgata tttattatat
    13261 ttacaacctt attttgatcc tccactcacc cgaatatcac tagcaacgaa ccaaacagag
    13321 tgttattctt ggctatagta aaagagcaat aaatctcact gctattaagt taagaattaa
    13381 cttctttttg cctgatacaa gcatcttgct aacgaagcta ttattcttgt cgcggcattt
    13441 tgaaagagga ctaatggatt agccatgtat gtttttcgcc gtttttcgaa gcatattatt
    13501 acttttctcc attcgcttga gctcgctttt cgatttttag gtcatttggt tcatagcact
    13561 atcaaagtta tttttggcaa actgtccatc gtatggccta acacactaga aatgatttat
    13621 tattctggag cgcggcttgt tattctactg agttttattg gtatattgtt aggcgctact
    13681 gtttcacaaa cggtttatgc gctcttaaaa ccttttcagc tacatcaacg agtactcccc
    13741 attgttcaaa atattttaac ccatgaaata ttaccggtac ttattggctt cattttatgt
    13801 atccaagccg ccttgcatat ggttaacacc cgtttggagc attaccagga gaatccagag
    13861 gcaattatcc ttgctcaagt attaccaatc attgtaggta tgaacattgc ctccttactg
    13921 ttatacgttt atcttgtatc tgctattttt tttagttttt atctcacttt tcattttatg
    13981 ctagggttta caaacaatga atttttatcc tatgttgtca gctcgacaac cttatttgat
    14041 ctgatatact cggtcgttaa gacattgatt ttatgcatca tagttgggtt gacttctggt
    14101 tattattatt atgaagcatc tattagacat atttatttga gaaaagcagt ttctcgaatt
    14161 ttaacgcgtg gctcattctg gctgatcatt gccagtatgt atattactct tacgttttag
    14221 ggacgacgtg cataacgaac gattctatac attagtgggt atttttatag taggcgccct
    14281 atgtttaatg attctaggcg gtactttttt ttatatagaa tacaaacgcg cacaaaaaca
    14341 aacttttgtc atgtttttca aaggctcttt aaaagggcta gtcactactg ccccagtaac
    14401 ctatcgaggc gtgaagatcg gtgaagtcaa agtcatagaa atcactgaaa ataaagagca
    14461 cagtaaagta cttattcctg tttacgtaca attttttgtc gagagaacct atggttttag
    14521 tcaagaccct attcacctgc ttattgataa tgggtatgta gcaaatatca ccaaacctaa
    14581 cctgttaaca ggcgttgctg aaattgagtt gattaagcca accccagcgg taaaatataa
    14641 acaaacatac taccattctt atcctgtttt tccgactcac aattcggctg aaaaatatac
    14701 atctatggaa gaagcgtttg aggcagcgaa aaaggctttt gaagacgtca gtgaattagt
    14761 tcgttccaaa gaaattcaag atactttaga ggcgatacaa aaagtttctg aaaatttagg
    14821 gcaactagcc tctagcttaa accaagatgt gcccagtgtt gttgcgtatt taaatcaaag
    14881 cttaaaacaa attaccagtg cagcttactc cactcaaaat ttgacagatt atttgtctcg
    14941 atatcctgag tctttactcc gaggcaagcg atgaaaaacc agaaacattt tttaaaattc
    15001 tattgcatcc gcgatcggag ttggccggct agtgcccgag cctcttttga aggcattcga
    15061 ttcctatctt tttcaaacta tgccgaattt cgcaagaaac ctgtgaagct cctcctccta
    15121 ttgctcattg tcttaaccct ctgtgattgc ggtcgaagca aagaaactca attttattta
    15181 ttaacaccta tctcccctca aaaaaatagt cataattcct ataatcattt acagattggt
    15241 gttgatgaag tgagtattcc tgattatatg aaaaaacctc aattaatgat tcaccagaca
    15301 cctcatcacc taactctcga agaatttaac caatgggctg gggccttgga taagaatatt
    15361 actcttgtat taactactaa tctttcaact ttaatgccag gagtagtagt tcagacctca
    15421 ccttggaaca ataaattcca cccggattat catcttcaag taatgatttc acaatttgaa
    15481 attgatattc atggtaacag tattttaaga gccgaatacc ttatttaccg aaaagaagag
    15541 ttaatctaca aaggaaatgc ttattaccat attaaaatcc ctttaatcag tattgaaacg
    15601 cttgtgcaga gcatgaatac gaaccttact catttaactg aagaaattgc acggtttttt
    15661 ataaaaaaca cgactaagta atatactcat agtgtcttca aattaaggaa aattattttg
    15721 attgaattca gctattataa tgctttaaag gcattatttt atgggataac aaagagactt
    15781 catgattggc aaagataaat gggaaatact gactgagaag atgaccagtc ttcatatcta
    15841 tgaagcagat ctcagcgaaa aatttatact aggcagtggc aaaggcggac aaaaactaca
    15901 taaaacggca tcaacagtct atttgaaaca tttgcctact ggtatggaaa taaaatgcca
    15961 ggaatccagg agtcgtgaag ataaccgcta ttttgcgagg caacggcttt gtgagaaatt
    16021 acaagccgta ttgagcgatg aaaaaacaaa gacacaacaa agaattgaaa agataaaacg
    16081 ccagaagaga cgacgctcga gaagagccaa acaaaaaatg ttggatgaaa aatcaaggca
    16141 gagccaactc aagacattaa gaaaaaaccc ccgatctcat gacattgaat aataggcaca
    16201 ttaaccaatt accattttag ttaaatcaaa ataccgggta acatccctaa agttttttgt
    16261 agactcctga actttaaccc agggacatta ttttcagttc gcttcgatca attccttctc
    16321 ccacgtctgg tgattttgaa attgaaattt ttatatcgtc aggttcgagt ctttgaaaag
    16381 gagataagac atctttttgc aatgctttga atttgcttta gtattgaaag gaatcattaa
    16441 attaaggtca gctccgatat ggcaggaaca agtttactaa cattgattga cgatattgca
    16501 acggcactgg atgacatagc tgtcatgact aaagtggcaa ctaaaaaaac tgccggggtg
    16561 ttgggcgatg acttggcact caacgccgag caaataatag gcatacatgc gaacagagaa
    16621 ttacctgtca taggagctgt agccaaaggc tctgctgtta ataaattaat attaattcca
    16681 ttagcgattt taataaattt tttcaccccc gcactggtgg gcatcctatt gattttgggc
    16741 ggattgtatt tgtgttttga aggttttgaa aaaattgtag aaaaaatttt ccataaaaaa
    16801 aatgagcctg caaaaaagaa aatagataca cagaccatcg atatgcgcca atttgaaaaa
    16861 gaaaaaatta gcggtgctgt acgaacggat tttgtattat cagctgaaat tattgtaatc
    16921 accttaggta ctgtggctgc gacatctctg attaaccaaa tagcggtatt aactttgatt
    16981 tcattaatta tgaccattgg cgtttatggt ttggttgcgt gcattgtgaa attggatgat
    17041 ctgggcttca ttttacggga aaaaaagcat ccgcttaaac aaattggaat tcttttgatt
    17101 ggagcagccc caatacttat gaaaatttta agcgtagcag gaacaattgc catgttttta
    17161 gtaggagggg ggattattac tcatcaaata actgccattc atcatttcac agaaaacctg
    17221 tctgcaatga tgtccatagg agttgatttt tttataggaa ttatttcagg tgcaattacg
    17281 gctgctctat attttacatt caagaagatg aaatcaaaat ttaaactaat gaatacgaag
    17341 taagtactat aagagtcgaa atccattgta aaatcaaagt aactttgcat tccatcgctc
    17401 aaatgactat agttaaacaa tgatttttta acaccaaacc tcatcaagtg attctttttt
    17461 tgcgatttat tttggatttt aataccacac tatcaatagg agatcagcag gatgccagat
    17521 atcaaacatt accttaaacc catggtttta ttttcaggac tattggttgc tccttttgcc
    17581 atggccgaag caacaaaagc tgaggaaact tctactgtaa aattaagtac tattcttcaa
    17641 cagttgtctg accaggggta taaaaacatt catgatgctg aaattgacga tggcgtattg
    17701 actgtcgaag gcagcaatga gcaaggcgtt aagtttgaaa ttcaccttga tccggccaca
    17761 ggcaagattt taaaaaagca agaacatcca caaccaaaat attcaatcat agacattacc
    17821 aaaaaaattg aagacgaagg taaattcaaa atcattgaaa ttgagtttga tgaaggtatt
    17881 tacaaaataa aaggtgaaac agacagccac gaaaaaaagg aactgcatat tgaccccaat
    17941 accggggaaa ccaaaacaga tcattaactc cacatgaaag agcaagtctt tcctttccct
    18001 tagcacaacc aattatttcc ctaaggaaaa ggaagaggct tgccccactc aaccactttg
    18061 gtcttctgat gaaggagtta aataacggct ggcccaggca gcaataacct ctgcagcata
    18121 ttgggagtcc cttttgtcac ttaacaaatg atcagcctta tcaagactga taaagctttt
    18181 tggatgcttg gctgctttat agattttttc ggcctcttta attgatacca ctttatctat
    18241 gggggagtgc aaaatgagta acgctttacc agcatcacta ttaatcgtgt cctgataccg
    18301 gtcaatatct tgcagaaatt gttttttgat agtaaacgag cgtggaccaa gcgtaacttg
    18361 agcttcacca tcagattcta ttttacttaa atcagcagaa aaatggtgct taacgtggta
    18421 ggcactggca ggcgcgccaa tagtggctat tgcctttact tcagaaatat tcttggctgc
    18481 aagcagtaca gcggcacctc caagactatg acctatcagc agaacaggag ctcgataatg
    18541 ggctcttaaa tagtcggcag cagcaactaa atcttcaaca tttgaagaaa aattagtttc
    18601 cgagaaggaa ccttcgctgc tgcccaaacc cgtaaaatca aagcgtaaca ccgcaaatcc
    18661 atttgagacc aaagcgctgg caattctgct tgcagcagct atatctttac cgcaggtaaa
    18721 gcaatgagca aataaaacat aaccaagaca tttccctgtc ggttcttcca gtttcccttc
    18781 cagcttcaag ttgttactgt aaaaatgaac ggttttgtac attgcatatt ccttatttat
    18841 cttgattagg tgctcataca gaacattcaa atctgtttat aaatcctttt tcttgctctg
    18901 aaattaattt gcttgattac tgatccagag tctggatagt ttcaaagtgt tcacatacta
    18961 acaaatatag tcatatctaa cccaatttcc caatctattt cgactcttta ttcattttgt
    19021 atcatggtgt cgcgaagtgt aatcaaagca acgatttatc tgcactacac cccacttaaa
    19081 gcgccgcttg tttaaaatca tgggagattg accgccctct tttcccatca ttatgattcg
    19141 cgccttaaga cccggaggtt tccagaaatt ttcatctatt tgaaataaaa ctaagaatgc
    19201 tatatgtttg ccagatattg atttattgcc aaatcattgt cacatcatga aaacataccg
    19261 aaaagaacta tggtttaata tcccggagcg tatgggattt attaatatca cagataaagt
    19321 cattgaatgc ttaaaggaaa gtagtattca ggaaggttta atattggtca atgccatgca
    19381 tatcaccgct tccgtattca tcaatgatga tgaaagcggc ttgcatcaag attataaaaa
    19441 atggcttgaa caaatagcac cccacgaacc cattaggcaa taccgccata atgacaccgg
    19501 cgaggataat gctgatgccc atatcaaacg acaaatcatg gggcgagaag tggtcgtcgc
    19561 tattacagaa ggaagactgg attttggccc atgggagcaa atattctatg gtgaatttga
    19621 cggccgccgt gataaaagag tccttgtaaa aattatcggt caataaatca ggactcgaag
    19681 gcatcatggg taacttaaga ataaaaggtc aggcatttct ttattctcta gtaagccatg
    19741 cttctaattc tcgctgagaa aaaaggaaat agtcgacctt actctactca gctttcatgg
    19801 atcatttcgt cggtaagtcc tattttcatt aattatcttt cttgattcta ctggggattc
    19861 attttttccg ccccaacaac taaaaatcga tggtaagcga cgtatattga ttgcttttga
    19921 taattcgctg ttgctattgc ttaattcatt atcgacatca aggccttttt gataacaagc
    19981 ttcaagcgcc cccaaaattg tattgagctt cgcctgactg cccatccagt aaggattgta
    20041 actcttactt cctgcttcca aacggttgat gacatgcaac aaggctacat gcttactgta
    20101 gatagtttta agattctcgc aagacagatc tttgtgggga ttatgaatct caccttttgt
    20161 tctggtttct ggttctgctt tggctctggc agttcttttt atctctggat gaaactcatg
    20221 attaagtgcg caatattcga ttgcagcaac ctcattctga gttctcaaaa tatatacgaa
    20281 attcgctgca tgaatgatgt tttgtgcact tgcattaaca agtaaaaata catagtttcc
    20341 attttctttt ccccaatttg catgggggac aagcagtccc ttaccactgg ctgttaactt
    20401 cccattgctg tcagtcactt gaaagtgatt ggattgagat gaataactaa cccaacctga
    20461 tttcgcagcc tcatagtaca ggacttgcca atgaccagcc cctccttgag taccagtccg
    20521 gaaaaaatag acactgcttt tattaagagt cgccagatcc ttttccaacg tttcctgatt
    20581 tttcccactt acccataatg ttttttcttc atctggactg aacataaagg caggacccag
    20641 gtcaacttga atatcttgaa cacctcgagt gtattcaaaa tcaagatcac tgcctaaaat
    20701 caactgggca gctttcttta tcatcatttc tgcacaattt tggtttaaga atacttcttt
    20761 ttttaaattc attggaaaat cccagtcact tttggattat tataatacaa aataatcaca
    20821 taaaaagcta agtgattgcc tggattatag aatcatccca atatttgagt cacctgcaag
    20881 acagcagaaa ggaacaaagc aaggaggggg aagtcttact cctttcctcg ccagtcaatg
    20941 ccccaaaagt cgtgttaaaa tcccaaagat agaagctccg tttaaaacag cttgattgga
    21001 attggttatg aaagagaaga ccgcgaaaca attgaaattg tttattatct atctacttag
    21061 ctattggcta ttaagcatta tttcatgcct tatcgctttc ggttatgatg attccctacg
    21121 catgttgctt gcctctccaa aatcagatct cagcggtgct ttattatttt tctcttcatt
    21181 tattgccacc gctttgttgt ttgtttttcg atacaagact ttttcagata aaccttatcc
    21241 ttattttatt tttgggttct acgtaggaaa cgtctcgtta ctgatgttat tcatattgga
    21301 tgcttttatc cgcgaactga ttgtatggaa attccctgaa ttcttgcttg tttttatatc
    21361 accattcgtt gaattagtgc tctcctatct attttttgga tttgcattcc tggcaatcat
    21421 tccggcagtt accagtgctt ttattctcta tgcagttcag aaaaagttat tattgccacc
    21481 aatataaacc tccagagttt tctctttttc atttgtaatc gaccccttgt tcttgccttg
    21541 gaaaaaagaa acctgcctct aatctattgc ccgatttatt tattccccac aaaaatatat
    21601 tttataatga aatgtatcat tttatattaa aacgctatat tatacgtttt ttggagaaaa
    21661 tatgggtata gtaaaaattt cggacgaatt gcatgaagca accaaattga tggcaagagc
    21721 catgagtcgg tcaatcaatt cacaagcaga atattggatt agaataggaa aattggcgga
    21781 agagaatcca tccatcacct atactgacat tcttaaaatt ttagtccatc aagcttcact
    21841 aggtgaaata tacgatgtta gtgaagacgc ctgaagaaat aaaaaaaatg cgcgttgcgg
    21901 gacatttggc cgcatctgta cttgaaatga tagagcctta tgtcattgca ggcgccagta
    21961 ccagggaact tgagcaagtt tgtcgtcagt atattataga ggatttgaag gcgattccct
    22021 cgacgttaaa ccactatggg tttcccgctt gcatttgtac ttcgataaat cacgtggtat
    22081 gccatggcat cccctcagag aaaaaactta aaaatggcga catcattaat attgatgtga
    22141 ctgtacaaaa ggacggttat attggggata ccagcaaaat gtttctcgtt ggcaacataa
    22201 agccatttgc caaaaagtta gtagaagtta cccaggaatg cctatacaaa gctatttcaa
    22261 tagttcgccc aggggctcat cttggagaca tcggaaacat aatccagaca cacgcagaac
    22321 aacatggata ttcggtagta cgagagtttg gtgggcatgg aattggtaaa tccatgtggg
    22381 aagatcctca cattatgcac ttcggcaaac caaataccgg attacgatta caagcaggaa
    22441 tgactttcac tattgagcca atgctcaatt tgggccgtaa agaggtcaag actttagggg
    22501 atggttggac tgttgtcaca aaagaccata aactatctgc acaatgggag catactattt
    22561 tagtgaccga taatggacat gaaatactga ccttaagagc ggatgagcat ctatagacat
    22621 ctctcctctt gcttagtaac tactgttccc ccaagcctcg cgacggaaaa catatactat
    22681 aatcaaacta aagaaaaccc agtcaatcaa gggaaatagc atgccttgcg aaaaatgcga
    22741 ttcagcggca acctaccatt tatcgggaac ctaccagtta ctcatcttac ccccacgagg
    22801 tcattgctat tcaaaattat taaagcattt aaccgaaaat aattttgata tcaataaaag
    22861 agacagcgga attatcgaat tattatttac tgctgagaga gcacaagaca taggccatgc
    22921 tttggagcga tgttattcac aagcggagct tgaggacagc aaagccgttt tgttgccaat
    22981 tgatatcgcg gacagaaaca tagagagagt actcaatcat acgcactcat taaaaaaaat
    23041 tgttgggttg tgtttgtctc aatggctggt tggactcatt gaaaatcagg ggctatcaac
    23101 cttctgccag cctattttgc ataataccac gctcaaaccc tatggttttg agtgcctgct
    23161 taggggaaag caggataata atatactgcc ctcctccgat ttatttggag cagcaaaatc
    23221 gagcgacatg ctttttttgt tagataagaa agcacgtata tgccacattg agaatatgag
    23281 caaaattgac atcgctgaca aaaaaatctt cattaatttt aacccaactg ccatttacga
    23341 tcctcttttt tgcttgacaa caacgaacca atccttaaaa aaaacgtcca ttaaacctgc
    23401 acaaattgta tttgaagtca ctgaaagtga tgaagtaaaa gataaaaaac acttgttaaa
    23461 aataatagat tattacagaa atcaaggtta ctgtgtcgcc cttgacgatt tgggctccgg
    23521 ttacagctct ttgaatttgc ttactgagtt acaacctgat tacgttaaac ttgatcaaga
    23581 attgattagc cagattcata caaatgaatt taaacaaatc atattggaaa aaatatgtga
    23641 gatggctacc aaactaaaga taaaaataat ctgcgaaggc atagaaacag aagacgagtt
    23701 acagattata aaaaattatg ccatcgattt ttatcaaggt tttttgtttg agagacctat
    23761 gccttacgaa aaaatacccg actatatcaa agcaagcgca taaaaatgga ttggctattg
    23821 gatgcatgac aaatgcttac aaacattgaa agagcaaaac ttcaattaat tgatgaaacc
    23881 agcgatatgg tcattataac caaagcggaa ccacttgatg cacctaatgg tccggaagtg
    23941 gtttgggtga ataaagcctt tgaaaacatt tcaaaatata aagccaatga aattattggc
    24001 caaacaccac gtatccttca aggtaaagaa accagtcaaa acacattaaa aatgataaaa
    24061 gaagcaatga aagctcaaac gaatatcaat gtggatttat taaactatac taaggatgga
    24121 acaccttatt ggattaattt ttcaataata tatttgagag acggtcaggg taacttatgt
    24181 tatctaggcg caatagaaaa agacatcacc gcaatcaaga atttaaatat tcaattatct
    24241 gaaaaagcaa agactgatac gcttaccaat gtttacaatc aggaaaattt cttcatcgca
    24301 gggaatgaag cggttaaaaa cttccatcaa aaccaggaaa cagttggatt attatttttc
    24361 gatgtagatg attttaaagg aataaacgat acgcaagggc atatctatgg tgataagata
    24421 ctaaaaaaag ttgcccagga atctttagag cttgtccgta aaacggataa cgtatttcga
    24481 tatggcggcg atgagtttgc tattattttt tcaggcatta acgtgaaaat cctggaaaga
    24541 aaagcacaac aactacaaaa aaagctggca aaatctcaca tctccgtgag catcgtatgg
    24601 actatttcaa agcaaagtga taaatcgata aacaccctct tggcgagagc ggatgaagca
    24661 ctctattacg tcaaagccaa taaaaaagga ttatttttta tcagagaata atcacatttt
    24721 gacgctgcca aattgcaagg aaactaaacc cagagacatt caattcaatg atatcgacct
    24781 ttaaacaatt cttttatgcc acgaaaaaat aattattttc agttttaatc gggtttctct
    24841 caaaagtcat tttgcataat catgtgttac atcatattgt tccttaatat tattgagggt
    24901 gagtctgact aaaagtctat tgatcaggac ttacgaaaaa ccccaagatc aaggcaaaaa
    24961 atgttgctaa taagggagtt tagataaact aaatgactga attaaaaaaa tttttaacaa
    25021 agagagtggg gtttttcgta agtcatgttg atgttgaatt aagctggttg ggaataaagc
    25081 tttttcaaga ccacttcaag agccttggca ctagctgaat cctgatcttc atgggcaaaa
    25141 tttgatgctt tttccaatgt tattgtggtc ttgttaaaca aacaatggcg tccggatcgt
    25201 cctccaaaaa agcctggttg ataattggca aagaaatgct tatgaaaagt tgggggagac
    25261 aggaggctaa acaaatcctc cgtggtatag gtatcatgag tgagtctccc gaccaggaat
    25321 ccccccagat agaacatcca aggactgtta tccaatttta atacagcgcg acctaatgag
    25381 tccgttggaa aattccactg catttccatg ggttttcggc ctacgctgtt taggttcgct
    25441 tcaatcaacg tgtatttaaa aaaaggagag cgtgacagtt gctgggcaag gccaccagag
    25501 attcctgctc gacaagattc aaggctcaat atcttggggg cttcctttaa cccattagta
    25561 gttaatcgtc tgattagatc atccgctgta tccccctgta tatacaatcc ggtatcctct
    25621 gctggtgttg gcgcaagtgt ggaatgtccc atgattgtca gatgatcttc gcatgtgact
    25681 tttcctaatt cctgatcgtt taataagata taaccatgat ccttggcatt ggtttgctgg
    25741 ccaagcaaag ttgcctcagc agactctaaa acataggatg gatgaaacca gatgtatcgc
    25801 ttcatacagg aaatctcttt tattaaacaa gatgaactca aacgatacaa atttatttgt
    25861 tttttgtaaa tgtaaaattc gatatttgtt gtctttgcaa atttaaaata atgtgcccag
    25921 ctcaggaaaa taacttgtga agccaagctt acaagaaagg cttgtccctg tttttatagt
    25981 ggtggcaccc aaaacccggg gttggcagcc ctccttccta ttgtattgtt ccggctcttt
    26041 acaggtattt ccaagttcat ggatgtcaaa gtgaaatgcc tttatcttga tggcttttaa
    26101 cattcacttt ttgagtcgac cggtcacaaa agtttggctg ttgtccaagg tcagggtgac
    26161 gatggcctct tgggtcggta tggcacttca taaagaaacc caggcgctca agacgctggt
    26221 tcatgttcct taattccatt ttagagtaga tcccttgtct attttcaact tgatttaacg
    26281 ccgtattgac agaacgcaaa tggccgtagt tgactcttag tgtttttagt aaatcattgt
    26341 agtgcacccg atccaaagac gcttccagcg atacgaacaa gtcagcaaaa aatgattttg
    26401 cactgggata tggacgctgt ggcgacctgg ccacagatgg cttgtcaact actgacgacc
    26461 cttgcattag tttattgaat aaacgcacat gaccattgga gctagcaaca cacattaaca
    26521 tattgtatcg ttcgctatag ggaagacacc tgtagtttgc aaccaaattg gggagctttt
    26581 tatacaaaac catacatgaa cttagcgaat atttcgcaag ctcgctatcc aataaaccac
    26641 attgtttcat ttcgcataac aaaaaagcta attctttgtt gcgaaataat tcacatccta
    26701 tctcagtctt atattccaaa aaaatttctg ttaataattt tttttgcgga gagctgtcaa
    26761 atacggcaaa atgacatttc cttttcctca gagcggtgag cagacaggtg gtttgtttct
    26821 gattattcca gggattgatt aaagtaacct catactctcc atcgtctaaa ggcttaatat
    26881 ttccaatacg tagtgcatga cgcctgtgcc tgcccaatct atccagcttc ccaaagttca
    26941 tccctacata aacaggaatc atggggtcta ttgttttaag cttaaccagc ttgcgcgggt
    27001 catagaaaga agtgacatga actcccaaaa gcttggctag aaatgcagta tcgctttgct
    27061 tggcaatggg attattgcga aattttgggt gtagtttatg agccaaaaga ctttcgccat
    27121 cccaggcaat ttggtagtag taagcactga tgtgctctaa aatttttact gcgagcgaat
    27181 tggaactgga tcctttttta ctgttatcaa tttcttttaa tttttcatta tcgataaaaa
    27241 taacatcttc atcagttctg ggatcataag aataagtata cttcccagcc attttttccc
    27301 actgcacctt catggtgttg tcagtgcgtt taattctagg tgtaacgccg gacgttgttt
    27361 gtttgaattt tttccgtaca acatcgaccc cttctgcttc accgttgagc aaacaatcca
    27421 ggcaagccag gagaaagcaa gacccactcc cgccttgttt tatggctaca ggctgtcctt
    27481 tttgcggaaa taatggcgtc attggttaca aatatacgag ttacttcaga tgcggatatg
    27541 ataaccgaag actcaaattt atcaaaataa tgcgcactta gggtatttgt atgccaaaaa
    27601 ataataaggc aggaaaaaaa gttgagccca caccacttta tctgacgtat tgtgaaattg
    27661 aaagtcggat caatatccga gaattcgacc ctggtaatgg gaaggtttaa gtgagcgaaa
    27721 acaaagctct gatagctcag gtaagaaagt attaaagaaa gcgagtcagg tacgaaaaat
    27781 tcaccttgaa tttataactc acagctcagc cagagatttg ccccgtattg agcacttatt
    27841 tatccccatc tcaggagttc aaatcaaata caccaagcca taatagtcta tcctatatgg
    27901 tataggagta tgtgagagat gcccttaggc aatctcatta tcatattaag ggataagaat
    27961 ttatgaaact caccctattt ttagtattag tgtggatctg cactgagtct tttgggacaa
    28021 ctatcatggt aaaaggactg ccatctcctt tagagtacag aaatgatctt tattattggc
    28081 ctccaaatgt tgctatcagc cctgacacta ctaatttatt cattacaatg gatggcatcg
    28141 acaaggtgtg tttcttgaat acaggactcc aaggtgggtt agagcaaatt tcagagatta
    28201 gtatcatcat caatggggca aaaaaggatt ggaactgttt cccatatacc acgactattc
    28261 ttgaggcacg cccatagaaa caccaaacca ccaatcgaat aggtgccgcc cccgtatcta
    28321 ctcttgtttg gataagctca cattgtccct ttataaccgt aaattgtcct tttaaaccat
    28381 ggctcaattg attttttaaa gtacaattgt tatatatttt tatcattgag gatttttggc
    28441 tggctttttg attttaattt gcacccaaat aacttttaac ctgaggatag ataatgaaac
    28501 ctataaaaaa cagtaaagaa caagaacata ttgctcccga agctcaagac cccagattga
    28561 gctacactga ggacgatcgt cgcccactaa cgagcacaac atcagcaaaa catcctagta
    28621 gaatgttcgg caaatcggaa gagcgctcag caagggagag ttacgatcaa tatggtaaca
    28681 atgttactac ctattggaca caaggtccct ggaattaatg cactagctaa tggcttgggg
    28741 cgtttccact caggccatgc atgccccaaa ttgcccatcc tgtttaatcc attaagatta
    28801 aaaataactg gtacccggga tagtaatccc ccccgatttt aattagcatc aaaagttgag
    28861 atttaaatag atatataatt aaataagttg caaaggtata ttgaatgtca atttttaaaa
    28921 aattcaaaaa attttacaga tcctcatcag agaacaggac acagatacac ctgtttcttg
    28981 gctttgttat aattccaata ataggcatga ctgttttata
//
LOCUS       DASLOE010000025        26348 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.025, whole genome shotgun sequence.
ACCESSION   DASLOE010000025 DASLOE010000000
VERSION     DASLOE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 26348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26348
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.025"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..446)
                     /locus_tag="U6309_000860"
     CDS             complement(<1..446)
                     /locus_tag="U6309_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4917 family protein"
                     /protein_id="HEN5504313.1"
                     /translation="MAKLNPISFEEALENSTNKNRSILLGNGFSISLCENFDYKYLYK
                     QAQKLADEGEISISKSIKNLFDDINTCDFEKVLDHLNITIETIKHYPKAELLNRTLNK
                     DKDNLIAAFYNTINSVHPKFQSDISPGTFIACLKILSNFNKIFTTN"
     gene            complement(678..6623)
                     /gene="traI"
                     /locus_tag="U6309_000861"
     CDS             complement(678..6623)
                     /gene="traI"
                     /locus_tag="U6309_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HEN5504314.1"
                     /translation="MLSLQPLDSADGAAAYYLDVVNYYENDSKSVRWLGDGAKILGIH
                     GQAVEKEQMIALLKGILPDGTQLGRIDKDGIHHRPGFDMTLTAPKSFSILLESGADPR
                     LGEVFDKAVEWFVEEMEKEFAQARQLVDGKIEYIDTRNLVIAAFRQPSSRANDPNSHT
                     HLVVQNMTHCPDGKWRSLASDMDGQKGVVEQIMKHHIYGGLKFRNKLANLTKELGYQL
                     ASDGDGLWEIQGAPEQVLTHFSKRRESIEALLDEKGWSGAKASSIAAQKTKLDKEIID
                     YEKWKEDIIKECQEMGFDPHQLVASSYKPQKNLFQTLKENIVERFYGKDNIEMTHARE
                     SVYVAIESISQQHAVFEERELKKEALKYVIASNKIIDETLINKAIDENIANQNLYTAR
                     HPFTQKPLLTTPWQLTLESEAIQRIENGKGAVSAICSKQKVSEFIKAKEQEMAFALSP
                     SQKKAMTTFLTSTDRFIAIQGYAGTGKTTMLRLTRELASLQGYEIRGITAGSAAANEL
                     RHKGGLNASTFARELGRLQNQKQDLSKTIFVVDEASMLSNPQGHKIIKLAEQFNTQLK
                     IIGDKAQLPSPSSGKLFSVIQDYGIKTVAMTDNLRQKDSELRESAIHAGRGEIYDAVD
                     KLTHVETLDTYLKRVDYISSKWLSLTPEERQNTLCFAPTHRNRQDITQILRNALIQEG
                     TLTGQEHLQPILKERNLTGIKLRNAAYYSQNDIIRFNHTIQRYNIKAGDYLTVGQVTN
                     KNKHNNTLILKHENGQAIKFKLSSLPEFRTENKDLERPVEVYRREQLSLMAGDQIQWK
                     RNSEHNGIRNSELATIRQINNEGITIITEDNQTLHLPHGAKELRHLDHGYVLTTYATQ
                     GKDKKRGLGLIESQNRFASTIQNYYVETTRGIWEMIVVTDDKDHLVKAITTNNSDKYS
                     SMDMVDSDTLKAHEARFKEHKNSIVLQNAIEKKLSKEQDWKGLEQTVETYVQCKQQGE
                     DRKAAKLAFAIVNDPKLYRLAKERLGFGVSTYRREALRFQTSKLLHSLPKSERQDFST
                     VRQYVFLNQQILKRSQHINAQSLDNGISNQNKQALQQQSAKRNAVAFVISRDLERYKP
                     YLQHFSIGELNRIGLPQHEYGKEFKKAQMRLESLGKQATRDLIRTNISLYLNAQGEEK
                     ERLAVQIKRESKLSHSFVLTHAKELNQKPESLWQSIHKDARLHSDKLFRNGLSAEGRL
                     AFDNIKAYKALQLELRENWSASLKEAGNSEGKSSNPKSIELLTRRNELAHELMQNKAM
                     PEIASYFKLDLAKLAVQTEKQQYRENIKQFTASKSNFKTRLAVINEIKNDITGHYPFI
                     KEANIESKTLSKYLRVTDRKERLSNISSPEKKDYQCFLTYKKASMQTYRLWQQAHLNK
                     ADGNVQNNKLISAAISQSSKRDALAHQLKGSKYLDSILSYEKGNKEKLLAQASNHQIK
                     LREIKELNEVVHTLSMQFPSVTNSNSAKEISAWKKNWLSLSSHMKQIEKGSGYQLALQ
                     EYPLNVSSVNTINKELENNYDFKPETITQKSLQSTNPVLQKIQQSAQFLDARIINEAL
                     MADPETTYKTIWGEPKTQNSRELRYSGGLIVSLKGKDKGLWHDFSDGIGGAPIQAIIA
                     RDKLSFKEALSQAASMVGINQLENLSIFIKAPISSKNELQKLNDLEKKNKVISAKSIW
                     DGSILAKGTLAEKYLKQHRGIESIDKMDIRFWPTGSQWKNCNEQGLLEDKVNKIPALI
                     IAARNEKNELTGVQRIYLDKQTASKNKFMDNPKLSKGIIDGSCGVIQKGMQGSRLYIA
                     EGFETGASIALADSKATVLCSFGVSNMKNLSPIIKKFHSKEVVIAGDNDGEFAKSQQA
                     IEKTINAFKQDNLSARAVFPDGLQGKAKTDWNDIHLSKGIAEIQKQLMRKDIGAISSI
                     VDKPIELPKTIADFVQIKSMKLSGSQIISIRDNAATIDNKRDLNQLVAAYNKSQQALE
                     KSGSFTPEKTLPTKTRSEIEMDR"
     gene            complement(6644..8536)
                     /gene="traD"
                     /locus_tag="U6309_000862"
     CDS             complement(6644..8536)
                     /gene="traD"
                     /locus_tag="U6309_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HEN5504315.1"
                     /translation="MMAVNPTKLFIRGGQIFLHNVRMFTQVGKKVSLAMLIVFFIFSV
                     IAFYFNTSAYQRYIGQQWVKAQAMTLIQSKAKQVVRMPTGESYPVYSAQIVKAPMVIG
                     IVNQLKQALLKSMIEALFASVMMLIVMVKWLQKRGENQSKAKIIRGSELVDENTLQKC
                     IKKTSRISPYRIGGVSLPFGSETQHIQIVGTTGSGKTVAIRDLLTTIQARGERAIIYD
                     KGGTYLSRFYREHQDVILNPLDTRGHSWNVWAECEDKADLEALAEAIMPMPINNTMDP
                     FWIKAARMIFVSTANELKNDPKRSNLMLLQYLLTADLGRIHHLLRHTESESLVSDKVQ
                     KTALNVKTVMATYLKSLLYLKDDGDIFSIRDWILNEQNNSCLFVSSDGRKHPTIRPLI
                     SAWINTATKELLSLAPDDNRRVWFILDELATLHALPFLAAVKSESRKFGGCFLLGHHG
                     ASQLRTIYGNDGAASLSNLCSTRLFLRLPEHTDAEHASRNIGTYEIEEVNESISYGAN
                     TMRDGISVSRQTKEKQLVLPTQIQVINDLQGYLRVKGEFPAAKIKLNYVDYPLIHEEF
                     IARDIDPDPLRQKVEQLVDTYSDPILAASHDDALEAATSTKKDITKPIAREKKLEEEK
                     ELVEFL"
     gene            8601..9065
                     /locus_tag="U6309_000863"
     CDS             8601..9065
                     /locus_tag="U6309_000863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504316.1"
                     /translation="MEKNYKGQVMISSLDELSPEQRKKLRLASMGIGSLVLLIVVLLF
                     LCFGDFPSNKDSLFFYCTDWLLFLVSPLAAFGLGASFGFDLMLSDLGFAFSFGSYGGS
                     SLNQPFSSHESPNYHRSNHYGSSVSINPSSGRPMSGSSGMDTSGNPYGTRSW"
     gene            complement(9069..11789)
                     /locus_tag="U6309_000864"
     CDS             complement(9069..11789)
                     /locus_tag="U6309_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HEN5504317.1"
                     /translation="MAVTMQIYTITGGELLKAFYNATAAMFHYDGILGIFRTALIIGG
                     VWTVGQFIVKRDVRSMFFFILKYAFVISFILTPTCNLQIHDRTDPLRPDLTVDNVPLV
                     LGVVGGLSSQLSDKITRLFEFFFHSPNDMDYSQTGMIMGAKLFLSASNIKITDPLFNA
                     NMQKFMQQCVFYDVMYGRYGLKDINQARDIWELVKSNASVARGFIYDGSFKSCMEAAS
                     LLDSAWSGVIEDAKSKYAGFAFGNHSDALANFEKYLPQAYNHLTAMSGDAAALIRQNM
                     MANAIRDGVFSMGARVNSKAAIDSFSASRAQDKIPAALSNIGLMSAYWLPMLQSLLFC
                     IVIGCFIFVLFFFPFPSGISFFQFYATLYVWLALWAPMFTVINYIMTILAQFSLSFVS
                     TGATTLAYQTGMNQVYENMAALGGYLVLATTTLSYMLISRHVGSFVSLTQQPTGIVQN
                     AASSAAEEAQMGNYSYGNTSFANHNGFNSSSFHNDQNARISLGGIETSLDSGSIARIS
                     RDGSETLTMATATSHTPLNIQLGESTRSAFSELSDSAKSAGFNKSIASSEHYAASLRS
                     LAELGETQSHGEQSGQGHQISTSAGFNAAASKISNLIDTFAHDHNISRDDATKVLSAA
                     SINLGGSFGFGTGSAVPGAKGNIEIGGNVRHERDKSESHQDARLMNEARRFSEEHHFT
                     DVVNEARQATKDDHFRTSDDFSGRLANNFSAGFDKSMQYRDEAVASFSESESYHRQAT
                     TSSEQTASINLDAQTGFIDWLSHHRAPNSQGTIGLQQAEWLIRHDPELAQSYARQFVG
                     EKTAQSISQFQKNHPINESQVHQKNEGFKSRIAGSAGVDHALGDYTNTFNDRAKNINA
                     GSVNKDSVSAVDKDFARAKSEMSKNVEGIVQDGSQVMQDVIVAQANKDVK"
     gene            complement(11795..13180)
                     /locus_tag="U6309_000865"
     CDS             complement(11795..13180)
                     /locus_tag="U6309_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HEN5504318.1"
                     /translation="MKKILLSCGLIGALGIGQVYSKSIGEDLTGYFKALNMGANVTEP
                     HAYQAQRAGFYTGGNLYARSSVRNLQIMRLSWPKVSAGCGGIDATLGGFSHIKGKEFV
                     DFTKNILNNSQGLAFQLALEQATPLLGNVATKLQNVATWVNQTNISSCATAESAVLGL
                     APRTRIAQQHACQSIGQSKNLFSDWAEARQGCGKGSSNYDYDRVINENSSNEQVVDNT
                     NVAWNALKKNGLFADDIELAQLMMTLSGTLILTRDAEGVHVQRLSSLVSNNNLISALL
                     RGGEVKIYQCDEKVKCLQPTLTSKNIDISHGLESKVRDLILDMASKIKDNVEQSEAAK
                     GLIESTQYPVMKMVSVQMAFMKDSAVIDTTRYSEAIAIDILFQYLNENLQLVKQAAGA
                     LQYPEAIMKEFQTDLTQARRDLVQMEGSAHQRMSMAMQMIQETQTIEQMLVGEFSSEL
                     TQSLSWANQMR"
     gene            complement(13177..13599)
                     /gene="trbB"
                     /locus_tag="U6309_000866"
     CDS             complement(13177..13599)
                     /gene="trbB"
                     /locus_tag="U6309_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HEN5504319.1"
                     /translation="MRNKLAIFLILGTLFSSSLYADIDNAPLHALAKHQGFFFFFSSS
                     CPHCQRFAPTLKRLSQHYGFSVVAISVDGGFLPSFPDAVMNEGQTKVFQVSVYPSLFL
                     INPKSQVASLVTEGTIDEGELTNRLLKISQIHDKEVPL"
     gene            complement(13589..14371)
                     /gene="traF"
                     /locus_tag="U6309_000867"
     CDS             complement(13589..14371)
                     /gene="traF"
                     /locus_tag="U6309_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HEN5504320.1"
                     /translation="MARWFFVLLVSVSPFLYAEQPYLNEHEEGWYWHKDPKEEVKNKP
                     QTESVAPSHVSKPADPDKTWKLIGNRVQQARAQAILNPTPENIARARRLQRLIVAQAN
                     LFSEKWMLDLLINPDQDESLINPSSSAARDIYNQQNSMQKEKAITQISQTSGLLYFYE
                     GGEPFSERMAQVVSDFANSYHMTVIPIAMTNRISPMLPNSRVDSGQATQMGVKHIPAV
                     FALNPVSKKTMPVAYGLVSQSELKENILMASNAFQSGDYNAQ"
     gene            complement(14364..16175)
                     /gene="traN"
                     /locus_tag="U6309_000868"
     CDS             complement(14364..16175)
                     /gene="traN"
                     /locus_tag="U6309_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HEN5504321.1"
                     /translation="MKGFVLVFLILCSQVFALDLNQAYQEGVQTGTSHTNQSIDLLKA
                     LDLSQFPGYQANLPQEHYYSGVTQASTGLEADSQTAVAQNDAGKAVGESFNHRPLYQV
                     NPASESMQKLNQIAENGDAIMHGQNTDKTTCSLKPKECHYSWQEKTCLSSKVLGVLHC
                     ARHLRLDVSPYKTESYSLYLRKGGMRNTPFKVSVNLAQADTCQQGKTPCYTIYKDLAP
                     APAILLPANCAMVKVSFLDEKKLVVVEQTATCANPTLSLSVGNCRFGRCTVPYVHSVS
                     MTVEIYESKEYWDDQCQHLQNKEKEGLCHITEPLTCTEPNQTRIIGDVPLTRPCWKER
                     ASYTCGGSQEQNTCDNLVNQGCEQTASTCVKEEAGLCTTHQQTYQCPINQCTDNQLLC
                     GEDAFCLDGNCAKHELTPANEDDFKKAMSTLSAASDASKDFDGNANFIFKGQLLECST
                     TMLHFKNCCSDKGWGLDLELAQCSDAEKKLGKARENKLVVPTGEYCFKRKKLPIGSVC
                     VDHHQTYCVFQSKLARMVQVQGRRDQLHIGFGQNKYSNCSGITPEQLQLIHFEAIDFS
                     EFYEEVKNKQKQPDYQQTANGISQRLNQFYNQGDING"
     gene            complement(16175..16843)
                     /gene="trbC"
                     /locus_tag="U6309_000869"
     CDS             complement(16175..16843)
                     /gene="trbC"
                     /locus_tag="U6309_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HEN5504322.1"
                     /translation="MKTIRMNTSFLVALFLLSFPALAEDEAYIASIEANTKNRANAFT
                     NEIKQISKSLETSEKSGQIAEFTGEMHQVIKTQEPIVTHLKKVSQVLVFLSFSMPEKS
                     LKAWLLQCKKSGATPVIRGLIHNSFKETMTAIQTLSKKTGIGMQLDPILFKTFDIAMV
                     PAVVYVKDTPACPANMDCKPVSYDSLYGDVSLDYALEKMRGDEQSEDPVLDQMILRLR
                     GELI"
     gene            complement(16859..17854)
                     /locus_tag="U6309_000870"
     CDS             complement(16859..17854)
                     /locus_tag="U6309_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HEN5504323.1"
                     /translation="MKAFFILIAFLASGLTQAGSCHGRFVNPITDVCWSCLFPFSLGQ
                     ANLVSSNHLPDTKNPKLPVCECPGNPIPRLGLTMGYWEPVNLVDVTRTPFCLVNLGGI
                     SLNFGKYYRKGAVETDSNLSNQSFYHVHWYKFPLMYWLNVLTDGICVEKGDFDIAYPS
                     ELDVMWNDDELGFVINPEAVLFGSLPAQAACAADSSTALLGSAIDKLFWCAGAQGSMY
                     PLTGHVQEHVGGVQASVLLSERMNFKLHRQGLLEDTVGLDDSTSSAKSICHTHYSPIL
                     PKSRYRYQMVNPLPTTNACYPLGRATTLWEAGHEYPFKGEDFGYLIWRKRNCCAF"
     gene            complement(17851..18459)
                     /gene="traW"
                     /locus_tag="U6309_000871"
     CDS             complement(17851..18459)
                     /gene="traW"
                     /locus_tag="U6309_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HEN5504324.1"
                     /translation="MKKLLLIICMLYLPFAEAKDYGVFGKTFEIAEIDFLEYIQQKMK
                     GMQVNGEWKTVQSEFKKRVKEHVARPTPIRLPRAEDNRTWFFNPSLAVPYDVKDSHGQ
                     VIVKQGTVINPLDRVGLSSTLLFFDGDDEAQVAWVTQELTQHQKVKLILTSGSVKEVA
                     NQLKQAIYFDLNGFLVAKFQIKYLPARVFQAGKRLQINEVTV"
     gene            complement(18456..18791)
                     /locus_tag="U6309_000872"
     CDS             complement(18456..18791)
                     /locus_tag="U6309_000872"
                     /inference="COORDINATES: protein motif:HMM:NF021213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI F-type domain-containing protein"
                     /protein_id="HEN5504325.1"
                     /translation="MFKTTQVSLLTALITSILVNGIFFSALRRPDIATIDILSITSQF
                     IKEEAQKNHSNLEKEAEIKAFSHRLESALQDLSHSKSLVLLPREAVIKGSTDYTATLM
                     TMIKLEHKS"
     gene            complement(18784..21336)
                     /gene="traC"
                     /locus_tag="U6309_000873"
     CDS             complement(18784..21336)
                     /gene="traC"
                     /locus_tag="U6309_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HEN5504326.1"
                     /translation="MLSTIKKARLAVKKTSTILSHATGSLLETLREELGMTDAHHKKQ
                     IEALLDTYCLAPYLPDEVYDSEADIYPYKQASHMMFECSTLIGASEETVTILTSILTD
                     ILPPESCFHALFWASPKIGHMIDEMEHQRNQGSEVLKALAHRRAEFYRKGVYSSLTKE
                     SNFVLRDFRLFLCISIPRKSIEDDRLLLGSLREDISNNLKSVNIICKELSIEEVISLK
                     REWLFPTDSLYEEKTNYDTNTEIRFQLTDIEASMRVKSNQIEIEREEDTRVIKCLSVR
                     QYPKSPVQWQLGDLFGKMFNTSLQISCPFLFSFAIRIKDKDKVMAALDAKYADEGQNA
                     KQPFIAKWVKNFNKKYAEIEELRERLSGDDSLVDSYYNVLLFTTPANVRRDVRRAKDV
                     FRGNQFDLRTPTGLQLQTLLASMPFAPAEGMFDDYKSFGRVRPLTSFNTVNLLPIQGE
                     WKGAGRFAQVYPQRRGQFTAIYFFDNVVRNYNVAIVAPPGKGKSFLMNDYIQSLLSQG
                     GFVYIIDVGGSYAKSCEMLNGQLIDFNHDTEISLNLFSTIDAQAKNDTAFKDVHNQLI
                     QLVGMMARPSGNVSDEERSHIEKAIEAMWRQHHNATTITLIAHHLESSNDPISQNIGR
                     LLYSYTKNGRYGSYFEPPCSLDFKRNLVILELGGLDGNKDLQRIVLKILMYQITNAMY
                     YTPKHIPKSCLIDESYELLSDNGTGGSANFVNEGYRRAPKYGGNFITVTHGLDDYENN
                     PTAKMCYDNAYYKIFLGASKSTIDTLKTSNQLDAYGERLIRSLKITNEYSEFLMMCEE
                     NSTVHRLIVDPFSRVLNTTRGPEVEALKRLMATGLPLADAISKVALEVYGYV"
     gene            complement(21348..21698)
                     /gene="traV"
                     /locus_tag="U6309_000874"
     CDS             complement(21348..21698)
                     /gene="traV"
                     /locus_tag="U6309_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HEN5504327.1"
                     /translation="MKILTTLLLLPCVVLTGCAKLNEDFDCPAPNGGTCKRMDQVYDM
                     VNGKGKSLAVTPSRNPLVMDGRPGQPVRYGEGVMPLWIAPYEDTDGNYHQANRVYSVV
                     KEGQWMQQSQLALK"
     gene            complement(21712..22998)
                     /locus_tag="U6309_000875"
     CDS             complement(21712..22998)
                     /locus_tag="U6309_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/VirB10 family protein"
                     /protein_id="HEN5504328.1"
                     /translation="MSIQLKKQQQIFLGIVIGVIVLVIACFALLGNKTVKSREVDKPA
                     YKKHSLASPISTATNESHWIEKTQNAWRAELAKSELFDKNLKALGDEKDKQSKLITSQ
                     KDELSEMKSTLSELKHQIVKLQMERTTPIDGTLKNNEPQNGLLPRADEAAASNSMEMD
                     SGFIQYTAKLNKKSKPRIKTPNNYVFSNTFAKAVVLQGADASAGVLSQANPDIMIFQI
                     VDDGVMPNKYRAPLKGCMVSASVIGDISSERGKFRTERLSCIHSNGTNIDIQVTGIIS
                     DSKNGIRGRAVWRDEPMVKKAFWGNFWESMGNIGQQYSTDYSTSPLGSVSTIQAGKIP
                     LAAVSSGGSGAAKMYAQYNIKRAEMYHPIIQLPPIVTVDVTFLKGFWLDGGTDTNAPE
                     QPEESQNSKVAFEAPAESETAEEKAANQFFYKNHSF"
     gene            complement(23000..23713)
                     /gene="traK"
                     /locus_tag="U6309_000876"
     CDS             complement(23000..23713)
                     /gene="traK"
                     /locus_tag="U6309_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HEN5504329.1"
                     /translation="MKQMAMIFSLGLLIATAEGAQIKTVRNYEDIFINASIKEPNNLM
                     LEDDRIQQIKAPGNTLVDACNGKANCKLIDDTTGILTFLPSPLYHTRAFTINLLTEKG
                     LFYNVRVEPKPIASQTIVFKSYRNPVIRARAPHTSSYEKVMVGFLRALVNGYLPEGFT
                     QTIPKNPSVYKAQKTQLKRLLTVNGNRMRGEIFELKNVTNQPIDAKESWFNWPGTKGV
                     ALAKTHLAPFETTRLYRIS"
     gene            complement(23716..24279)
                     /gene="traE"
                     /locus_tag="U6309_000877"
     CDS             complement(23716..24279)
                     /gene="traE"
                     /locus_tag="U6309_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HEN5504330.1"
                     /translation="MKIEQKKSELMKEKKIRVLFIGISSVLLAMTFMQSITIVVLLHY
                     AHSKHETHFIPPKISQEFTLSGTGVSEGYLRDMTNFLTQLRFNITSSSATYQFNALLG
                     YVAPSLYGDIRAQLVKEVEQINHEHLSSVFYPSTFEIDIKHLSVKVMGQMKRFVGADL
                     MSDVRETFLIRYSYEYGLLKLTNLEKV"
     gene            complement(24283..24576)
                     /gene="traL"
                     /locus_tag="U6309_000878"
     CDS             complement(24283..24576)
                     /gene="traL"
                     /locus_tag="U6309_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HEN5504331.1"
                     /translation="MEDPRKYKTPQYLNEPYRLIIFTLDEVMVGALTLYVFGAVCGFF
                     VTSLILTCAFLFLMKRMKGNEGPYFYIHLFYWFFSLSPKLRATPPSWIRAFLG"
     gene            complement(24578..24880)
                     /locus_tag="U6309_000879"
     CDS             complement(24578..24880)
                     /locus_tag="U6309_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEN5504332.1"
                     /translation="MSLLNQFRSNVLSSINAHIGKIFILICLLIMAQGTFAADALSAA
                     EEVVKDTYNGSIKTYLYVGEAVAAVVTLMFTRNIKTLGGVGAMAIFLNVVAMLAGV"
     gene            complement(24895..25131)
                     /gene="csrA"
                     /locus_tag="U6309_000880"
     CDS             complement(24895..25131)
                     /gene="csrA"
                     /locus_tag="U6309_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEN5504333.1"
                     /translation="MLSLTRRVGESIVIGEDIFITVLCCKGNQVRIGFNAPNSVAIHR
                     YEIYQKIQSEKHDGLADPTKKFCPSMQQSILNHH"
     gene            complement(25145..25522)
                     /locus_tag="U6309_000881"
     CDS             complement(25145..25522)
                     /locus_tag="U6309_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vir protein"
                     /protein_id="HEN5504334.1"
                     /translation="MTTSSTLAPTEEGRKRIDRLFLRFAAMYGQVWRSQFKSDEFLVF
                     VKGEWQQGLFTYADNILDMAIDLCRKNKELPPTLPQFIDFCKNCSKRSSFFVPDAAPK
                     NNNPEVAKTQLLKMKHILNMKVN"
     gene            complement(25470..>26348)
                     /locus_tag="U6309_000882"
     CDS             complement(25470..>26348)
                     /locus_tag="U6309_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504335.1"
                     /translation="FLINDKELAALCGMPHLQQLAYLRGIRPYMDVKTGLVGIKRGIS
                     YQSIAEQLYIEPHQGIKSGSYSRMQIRRALSALGRAGLITPQSEGLKLILRCELATMG
                     YSVQNKLDLNQTQQLDPAKNQQRLINTGLSKTDPLKAGIDKSPKADTPLKEDNYYIFL
                     LSQFEKFWDLYPLKKSKQKAWEAFQALNLTPELPIQIYSALQQQIQFYQQQHSQGQWV
                     AAWKYPANWLAQHCWEDEIQSDTCQETNNAVHETNYKKQPIRDSFWESCKSGIEEETD
                     DNIIDLSAYRGRSQAH"
BASE COUNT         7242 a         5518 c         5137 g         8451 t
ORIGIN      
        1 tagtttgtag taaatatttt gttaaagttt gataatattt ttaaacatgc tatgaaagta
       61 ccaggagaaa tgtcgctttg gaatttggga tgaacagagt ttattgtatt gtagaaagcg
      121 gcaattagat tatccttatc cttattgaga gttctgttga gtaattccgc tttaggataa
      181 tgttttatgg tttctatagt tatatttaaa tggtccaaca ctttctcaaa atcgcaggta
      241 ttgatgtcat caaatagatt tttgatgctc ttacttatgg atatctcccc ttcatctgct
      301 aacttttgtg cttgtttgta tagatattta taatcaaaat tttcacataa actaatactg
      361 aagccattac ccaacaatat ggatctattt ttgttggttg aattctctaa tgcttcttca
      421 aacgaaattg gatttagttt ggccatcctt tgccctctat aaataagacc cgatgtgttt
      481 aattatagtt caaacgggta ttttttgagt ctgaggcact gaattaccga agtggttatg
      541 tcccttgatt gtcccaatat cctttggggg ttctctaaga ttatgtgttt aaaacagaaa
      601 aataatatga acacctgatt tagtatcaat ggcagatatt ttcagtgtga gcatacgcta
      661 tgctatggtt ggtgtatcta acgatccatt tctatttcac tccttgtttt tgtgggcagt
      721 gttttttcag gagtgaagga gccagatttt tctaatgctt gttgactttt gttgtaagct
      781 gcgactaatt gatttaagtc tcttttattg tcaatggttg cagcattgtc tctaatggaa
      841 atgatttggc tgccgcttaa cttcatggac tttatttgta caaaatcggc aattgttttt
      901 ggtagttcaa ttggcttatc aacgattgag gaaatggctc ctatatcctt tctcattagt
      961 tgcttttgga tttctgcaat ccctttgctt aaatgaatat cgttccagtc tgtttttgct
     1021 tttccctgca acccatcggg aaatactgct ctggcactta agttatcttg tttaaaggca
     1081 ttaattgttt tttcgatggc ttgttgtgat ttagcgaact ctccatcatt atctcctgca
     1141 ataacaactt cttttgaatg gaattttttg atgattgggc ttaagttctt catgtttgaa
     1201 acgccaaagg agcataaaac tgtggctttg ctgtccgcga gagcgatgga agcacctgtt
     1261 tcaaaacctt cggcgatata aaggcgggat ccttgcattc ctttttgaat aacgccacaa
     1321 ctgccgtcta tgattccttt ggatagcttt gggttgtcca tgaatttatt tttactggct
     1381 gtttgcttgt ccagataaat tcgttgaacg cctgttagtt catttttttc atttcttgca
     1441 gcaatgatta atgctgggat tttattaact ttatcttcca ataatccttg ttcattgcag
     1501 tttttccatt gagatccagt gggccagaaa cgtatatcca ttttatcaat ggattctatg
     1561 ccccgatgtt gttttaaata tttttcggcc aatgttccct ttgcaagaat agaaccatcc
     1621 cagatacttt ttgcagaaat tactttgttc ttcttttcaa ggtcatttaa tttttgtaat
     1681 tcatttttac tggaaatggg ggcttttatg aatatggaaa ggttttctaa ttgatttatg
     1741 cctaccatgg acgcagcttg agagagtgct tctttaaagg acagcttatc tctagcaata
     1801 atcgcttgaa tcggcgcgcc tcctattccg tcactgaaat catgccataa gcctttgtcc
     1861 ttgcctttca agcttacaat taagccacca gaataacgta actctctgga gttttgtgtt
     1921 tttggctcac cccagattgt tttataggta gtttctgggt cggccatcag agcttcatta
     1981 ataattctgg catccaaaaa ttgtgcagat tgttggattt tttgtaagac cggattggtt
     2041 gattgcaatg atttttgggt gatcgtttct ggcttaaaat cgtagttatt ttctaattcc
     2101 ttattaattg tatttacaga tgacacattc aatggatatt cttgcaaagc taattgataa
     2161 ccgctgccct tttcaatttg cttcatatga ctggaaagag ataaccagtt ctttttccag
     2221 gctgatattt cttttgcaga attagagttt gtgacgctag ggaactgcat tgatagtgtg
     2281 tgtacgactt catttagctc cttaatttca cgcaatttta tttggtggtt gctagcctgg
     2341 gctaacagct tttctttgtt gcctttttca taggataaaa ttgaatcaag gtactttgag
     2401 ccttttaatt gatgagccaa tgcatcacgt tttgatgact gactgattgc tgctgatatc
     2461 agcttgttat tttgtacatt gccatcagct ttattaaggt gcgcctgctg ccaaagcctg
     2521 taagtttgca tactggcttt tttgtaagtc aggaaacatt gatagtcttt tttctcaggt
     2581 gaagaaatat tagataatcg ttcctttctg tcggtaactc gtagatattt actaagggtt
     2641 ttagattcaa tatttgcttc tttaataaag ggataatggc cagtgatatc atttttaatc
     2701 tcattaatta ctgcaagacg tgttttgaaa ttagacttac ttgccgtaaa ttgcttgatg
     2761 ttttcccgat actgctgttt ttctgtttgt actgctaact ttgccaaatc cagcttgaaa
     2821 taagaggcta tttcaggcat ggctttgttt tgcatcaatt cgtgagctaa ctcattgcgg
     2881 cgggtgagta actcaataga ttttggatta ctactcttcc cttcactgtt tcccgcttct
     2941 tttaaacttg ctgaccaatt ttctctaagc tcaagctgta atgctttata ggctttgatg
     3001 ttatcgaacg ccaatcttcc ctcagcactt aatccatttc gaaacaattt atcgctatgc
     3061 aaacgcgcat ctttatgtat ggattgccat aatgattcag gcttttgatt caattctttt
     3121 gcatgggtta aaacaaaaga atgtgataac ttggattctc ttttaatttg gactgcaagc
     3181 ctttctttct cttcgccttg tgcgtttaaa taaagcgaaa tattggtacg tattaagtcc
     3241 cttgttgcct gcttgccaag tgattccagg cgcatctgtg cttttttaaa ttccttgcca
     3301 tattcatgct gtggcaatcc aattcggttt agttctccga tggaaaaatg ctgcaaataa
     3361 ggtttataac gctccagatc cctactaatt acaaaagcta ctgcattgcg tttagcggac
     3421 tgttgttgca gagcttgttt attctgattt gaaattccat tatccaacga ttgggcattg
     3481 atatgctgac ttcgtttaag gatttgttgg tttaaaaaca cgtactgcct gacagtagaa
     3541 aaatcctgcc tttctgattt gggtagtgaa tgtaataact tcgaggtttg gaaacgcagg
     3601 gcttcacgcc gataggtact aacaccaaaa cccaagcgtt ctttggcaag gcgatacaat
     3661 ttgggatcat tcacaatggc aaatgctaat tttgccgctt ttcgatcttc cccttgttgt
     3721 ttgcattgaa cataggtttc tactgtctgt tccaagccct tccagtcctg ttctttcgat
     3781 aatttttttt caatggcatt ttgtaagact atgctgtttt tgtgctcttt aaaacgtgct
     3841 tcatgagctt tcagagtatc tgaatcaacc atatccattg aggagtactt gtcactgtta
     3901 ttggtggtaa tggctttaac cagatgatct ttatcgtccg tgactactat catttcccaa
     3961 ataccacggg ttgtttccac gtaatagttt tggatggttg atgcaaatcg gttttggctt
     4021 tcaataaggc caagacctct ttttttatct tttccctggg tagcataggt cgtgagtaca
     4081 tagccatgat caagatgtcg tagttctttt gctccgtgag gtaagtgtag ggtttgattg
     4141 tcttccgtta tgattgttat tccctcattg ttgatttgtc tgatggttgc caattcagag
     4201 ttgcgaatac cattgtgttc agaattacgt ttccattgaa tttgatcacc ggccattaga
     4261 ctcaattgtt ctcgccgata tacttcaaca ggccgttcca aatctttgtt ttcagttcta
     4321 aattcaggta gtgaggacaa tttaaatttt atggcttgac cattttcatg cttaagaatc
     4381 agtgtattat tatgcttgtt tttgttagtg acctgcccaa cagttaaata atctcctgct
     4441 ttgatgttat accgctgaat agtatgatta aaacgaatga tatcgttttg actataataa
     4501 gctgcatttc tgagcttaat tccagttaaa ttgcgttctt ttaatatggg ttgcagatgc
     4561 tcttgtccgg ttagtgtccc ttcttgaatt aaagcgtttc gtaaaatctg agttatatcc
     4621 tggcgatttc tgtgcgttgg ggcaaagcat agtgtgtttt ggcgttcttc tggtgttaat
     4681 gacaaccatt tacttgaaat ataatcaacc cgttttaaat aagtatcaag cgtttccaca
     4741 tgagttaact tatcaacggc atcatagatt tcacctctac cagcatgaat tgctgattcc
     4801 cttaattctg agtctttctg gcgaagatta tccgtcatgg caacggtttt aatcccataa
     4861 tcttgaatga cagagaataa tttcccgctg gatggggaag gaagctgggc tttatcgcca
     4921 atgatcttta actgggtatt aaactgttct gccagtttga tgattttatg tccttgtgga
     4981 ttagataaca tagaggcttc gtccaccaca aatatggttt tacttaagtc ctgtttctga
     5041 ttttgcaatc tccctaactc tcttgcaaag gtagaagcat ttaaccctcc tttatggcgc
     5101 agttcattgg cggctgccga gcctgcggta ataccacgaa tttcatagcc ctgaagagat
     5161 gctaattcac gagtcaaacg tagcatcgtg gttttaccag ttcctgcata cccttgaatg
     5221 gcaataaatc ggtctgttga ggttaggaag gttgtcatag cttttttctg tgaaggggat
     5281 aaagcaaatg ccatctcctg ttcttttgct tttataaact cactgacttt ttgtttcgaa
     5341 caaatagcag aaacagcacc tttaccattt tcaatacgct ggatggcctc gctctcaagt
     5401 gttagttgcc agggcgttgt caataaaggc ttttgtgtga aggggtgtct tgccgtatac
     5461 aggttttgat ttgcaatgtt ttcatcaatg gctttattaa ttaaggtttc atcgattatc
     5521 ttattgctgg cgattacata ttttagcgct tcttttttaa gttccctttc ctcaaacacc
     5581 gcatgctgtt gactgattga ttcaatggcc acatacacag actctctggc atgagtcatt
     5641 tcgatgttat ccttaccata aaagcgctca acaatgtttt cctttaaagt ttgaaagaga
     5701 tttttctgag gcttatacga tgaagccact agttgatgtg gatcaaagcc catttcctgg
     5761 cattctttga tgatgtcttc tttccatttc tcataatcaa tgatttcttt atccaatttt
     5821 gttttttgag ccgcaatcga ggatgcttta gcaccactcc agcctttttc gtcaagtaat
     5881 gcttcgatgg attcccttct ttttgaaaaa tgagttaata cctgctcagg tgcaccctgg
     5941 atttcccaca aaccatcacc atcagatgcc agctgatagc caagttcttt ggttaaattc
     6001 gctaatttgt tacgaaattt aagcccgcca tagatatggt gcttcatgat ttgttcaacc
     6061 acgccttttt gaccatccat gtcactggca agcgagcgcc acttcccatc tgggcagtgg
     6121 gtcatatttt gaactacaag gtgcgtatgt gaattaggat cattggcacg agagcttggt
     6181 tgacgaaatg ctgcaatgac taaatttctg gtatcaatat attcaatttt gccgtcaaca
     6241 agttgcctgg cttgtgcaaa ctccttttcc atttcttcaa cgaaccattc tacggcctta
     6301 tcaaacacct ctccaagcct tggatcagca ccactttcaa gtaaaattga aaaagacttt
     6361 ggggcagtaa gggtcatatc aaaaccgggg cgatgatgaa taccatcctt atcgattcga
     6421 ccgagttgag taccatcagg taatatgcct ttcagaaggg caatcatctg ttctttttct
     6481 actgcttgcc cgtggatccc aagaattttc gctccatctc ctaaccaacg aacactttta
     6541 gaatcgtttt cataataatt gacgacatct agatagtagg ctgcggctcc atcggcactg
     6601 tctaatggct gaagacttaa catgcttacc ccccaatttg ttcttataaa aactcaacaa
     6661 gctctttttc ttcctcaagc tttttttctc ttgcaattgg ttttgtgata tctttttttg
     6721 ttgaagttgc agcctctaaa gcatcatcat gggatgcggc aaggatggga tcgctgtagg
     6781 tgtccaccaa ttgctctact ttttgacgaa gtggatcagg atcaatgtct cgtgcaataa
     6841 attcttcatg aatgagagga taatcaacgt aattaagctt tatttttgcc gcaggaaatt
     6901 cccctttgac gcgcaaatat ccttgtagat cattgattac ctgaatttgg gttggtaaaa
     6961 ccaattgttt ttctttagtt tgacgagaaa ctgaaatccc atcacgcatg gtgtttgccc
     7021 cataactgat ggactcattc acctcttcaa tttcgtatgt accaatattg cgggaggcgt
     7081 gctccgcatc agtatgttcg ggtaagcgta ggaacaatcg agtcgaacac agatttgaaa
     7141 gggacgcggc accatcgtta ccataaatgg tacgcaactg ggatgcaccg tgatgaccta
     7201 ataagaagca accaccaaat ttgcgacttt ctgatttaac tgcggctaaa aaaggtaagg
     7261 catgcaaggt agccagctca tccaaaataa accaaacacg gcggttatca tcaggcgcta
     7321 aacttaagag ttctttggta gcagtattaa tccaggccga aattagtggt ctgatggttg
     7381 ggtgtttgcg cccatcagaa ctgacaaaca aacaggaatt attctgctca ttcaaaatcc
     7441 agtcgcgaat cgaaaagatg tccccatcat ctttcaaata cagtaaggat tttaaataag
     7501 tagccattac ggttttcaca tttaaagcag tcttttgtac tttatcggat accagagatt
     7561 ctgattcagt atgacgcagt aaatgatgaa ttcgacctaa gtctgctgtg agcaaatatt
     7621 gcaataacat cagattggag cgtttaggat cattttttag ctcattggca gtcgagacga
     7681 aaatcatacg cgcggcttta atccaaaagg gatccatggt gttattgatg ggcatcggca
     7741 tgatggcctc agcaagtgct tcaagatctg ctttgtcttc gcattctgcc cagacattcc
     7801 aggaatggcc tctcgtatct aagggattta aaattacatc ttgatgttca cgataaaaac
     7861 gggataaata agttcctcct ttatcataaa taatcgcgcg ctcaccccta gcttgtattg
     7921 tcgtcaatag atcacgaata gcaacagttt taccagatcc ggtggttccg actatttgta
     7981 tatgctgggt ttcagaacca aagggcagag atacaccacc aatccgataa ggagaaatcc
     8041 tgcttgtctt tttgatgcac ttttgtaacg tgttttcatc aactaactca ctgccgcgaa
     8101 tgatcttagc tttactttga ttttcccctc ttttttgcaa ccacttaacc atgacaatta
     8161 acatcatgac agaggcaaat agtgcctcta tcatgctctt aagtagagct tgttttaatt
     8221 gattaacaat gccgatgacc atgggagctt ttacaatttg cgctgaatac actggataag
     8281 attcacccgt tggcatgcga accacttgct tggctttgct ctgtattaaa gtcattgcct
     8341 gggctttaac ccattgctgg ccaatataac gttgatacgc actggtatta aaataaaaag
     8401 cgatgactga gaaaataaaa aaaactatca gcatagcaag gcttactttc ttacctacct
     8461 gtgtgaacat gcggacattg tgcaaaaaga tttgaccgcc gcgaataaat aattttgtag
     8521 gatttaccgc catcattgac tccgaatcga tataaaaaaa gtgaaaatca ggctacaatg
     8581 aataaaaagg agaaaatcca atggagaaga actataaagg gcaagtgatg ataagctcac
     8641 ttgatgaatt gtcccctgaa caaagaaaaa aactgcgttt ggcatcaatg ggaatcggtt
     8701 ctcttgttct tttaattgtg gtgttactat ttctttgttt tggtgatttc ccaagtaaca
     8761 aagactcctt atttttttac tgcaccgatt ggctcttatt ccttgtgtca cctttagcgg
     8821 cttttgggct tggtgcctct tttggctttg atttgatgct ctcagatcta ggctttgcat
     8881 tttcttttgg atcctacggt ggttcatcac taaaccagcc gttttcctca catgagtcgc
     8941 ccaattacca tcgttcaaac cactacggct cttctgtgag cataaaccca agctcaggtc
     9001 ggccgatgtc aggaagcagt ggtatggata cctctggaaa cccttatgga acccgatcct
     9061 ggtaagcatc attttacgtc cttgtttgcc tgggcaacga tgacatcctg catgacttgc
     9121 gacccgtctt gcactatgcc ctcaacattc ttggacatct ctgacttggc gcgtgcaaaa
     9181 tccttatcaa ctgcactaac cgaatcttta ttgacgcttc ctgcattgat gttctttgct
     9241 cggtcattga acgtattggt ataatcccca agggcatgat caacccccgc actacctgca
     9301 atacgacttt taaatccttc atttttttgg tgcacttgac tttcgttaat aggatgattt
     9361 ttttgaaatt ggctgattga ttgcgcggtt ttttcaccaa caaattgtct tgcataagac
     9421 tgagctaatt caggatcgtg acgaattagc cattctgcct gttgcaaccc aatagtgccc
     9481 tgactattag gtgcacgatg atgtgataac caatcaataa atcctgtttg agcatcaaga
     9541 ttaatagatg ctgtttgttc gctggaagta gttgcttgtc ggtgataact ctctgattcg
     9601 gaaaaactcg ccacagcttc atctcgatac tgcatagatt tatcaaagcc tgctgaaaag
     9661 ttattagcca agcgtccact aaaatcatca gaagtgcgga aatgatcgtc cttggtggct
     9721 tgtcttgcct cattcaccac atctgtaaaa tggtgttcct cactaaagcg ccgcgcttca
     9781 ttcatcaagc gtgcgtcttg atgggattct gatttgtccc gttcatgacg cacatttccc
     9841 ccaatctcaa tattaccctt tgccccaggt actgcagaac cggttccaaa accaaacgat
     9901 cctcctaaat taatactggc agcacttaaa actttagtcg cgtcatcccg agaaatatta
     9961 tgatcgtgag caaaggtatc aatgagattg gatatttttg atgccgccgc attaaagcct
    10021 gctgaagtgg atatttggtg tccctgccca ctttgttccc catgtgattg cgtttcacca
    10081 agctctgcca ggcttctgag tgatgccgca tagtgctctg atgaagcaat tgatttattg
    10141 aacccagcac ttttagcact gtcggataat tcagaaaatg cagatcgcgt gctttctcct
    10201 aattgaatat taagcggggt atgcgaggtc gcagttgcca tcgtcagtgt ttcactgcca
    10261 tcacgcgata ttcgcgcaat tgaaccacta tcaaggcttg tttcaatacc acccaatgat
    10321 attctggcgt tttgatcgtt atgaaaagaa ctgctattaa acccattatg attagcaaaa
    10381 ctggtatttc cgtagctgta atttcccatt tgtgcttctt ctgccgcaga ggaggcggca
    10441 ttttgaacaa tacctgttgg ttgttgcgtg agtgatacaa aactacccac gtgtcgactg
    10501 attaacatat aactcaatgt ggttgtcgcc aagactaagt agcctcccaa ggcagccata
    10561 ttttcataga cttgattcat gcctgtttgg taggccaaag tagtcgcccc tgttgacaca
    10621 aaggacaatg aaaactgcgc caaaatagtc atgatgtagt taataacagt aaacatcggt
    10681 gcccataggg caagccagac atacagtgtt gcgtaaaatt gaaaaaaaga aatgccggag
    10741 ggaaagggga aaaaaaacaa gacaaaaata aaacacccta taacaatgca aaataaaaga
    10801 ctttgcaaca tgggcaacca ataggcggac attagtccaa tgttgctgag tgccgcgggt
    10861 attttatcct gtgctcgact ggctgaaaaa ctgtctatgg ccgcttttga attcacacgt
    10921 gcgcccatag aaaacacgcc atcgcgaatg gcgttggcca tcatattttg ccgaataagt
    10981 gctgcggcat cacctgacat ggcggttaaa tgattataag cttgaggtaa gtatttttcg
    11041 aaattagcta gggcatctga atgattgcca aaagcaaaac ctgcatactt agatttggca
    11101 tcttcaatca cgccactcca ggcactgtct aataaactag cggcttccat acacgattta
    11161 aatgaaccat cgtaaataaa cccacgagca acagaggcat tggatttaac caattcccaa
    11221 atatctcttg cttgattaat gtcttttaag ccatatcgtc catacatcac atcatagaac
    11281 acgcattgct gcatgaactt ttgcatgttt gcattaaaca acggatcggt tatttttata
    11341 ttacttgctg ataaaaagag cttcgcaccc atgatcatgc ctgtttggct gtaatccata
    11401 tcgttaggtg aatgaaagaa gaattcaaaa agccgggtta ttttatcact tagctgactg
    11461 cttaatcctc ctacaacacc caaaactaag ggcacgttat caaccgttaa atctggtcgc
    11521 agaggatcag ttctatcgtg gatttgcaaa ttgcaggtag gtgttagaat aaatgaaata
    11581 acaaaagcat attttaaaat aaagaagaac atactgcgca catcacgttt tacaataaat
    11641 tggccaacag tccaaacccc accaataatc agtgctgttc taaaaatccc aagtattcca
    11701 tcatagtgaa acatagctgc ggtggcgtta taaaacgcct taagtaactc accaccggta
    11761 atcgtataaa tttgcatcgt aacagccatt tcactcacct catttgatta gcccaactca
    11821 aagactgagt gagctctgat gaaaattcgc ctacaagcat ttgctcgatg gtttgagttt
    11881 cttgaatcat ctgcatcgcc atgctcattc gctgatgcgc gctaccttcc atttgaacca
    11941 agtcgcgacg agcctgggtg agatcagttt gaaactcttt cattattgct tctggatatt
    12001 gcagagctcc tgctgcttgt ttaacgagtt gcaggttttc attcaaatat tgaaataaaa
    12061 tatcgatggc aatggcttct gagtaacgtg tagtgtcaat cactgcactg tccttcataa
    12121 aggccatttg aacgctgacc attttcatca ccggatattg tgtggactca attaatcctt
    12181 tagcggcctc actttgctcc acattgtctt taatcttgct tgccatatcc aaaatcagat
    12241 cacgaacttt tgattcaaga ccatgactga tatcaatgtt tttactggtt aaggtgggtt
    12301 gtaagcattt gaccttttca tcacattgat agatttttac ctcgccaccg cgtaaaagag
    12361 cgctgattaa attgttatta cttactaagg aagacaggcg ttggacatgc accccttctg
    12421 catcgcgagt cagaattaag gtgcctgata aggtcatcat gagttgagct aattcgatat
    12481 catctgcaaa taagccgttt ttctttaacg cattccacgc cacattggta ttatcaacta
    12541 cctgctcatt gctgctgttt tcattaatca ctctgtcgta atcataatta ctggagcctt
    12601 taccacagcc ttgtcgtgcc tcggcccagt ctgaaaagag atttttggat tgaccgatgg
    12661 attggcaggc atgttgttgt gcaatacggg tgcgtggggc taaacccagt acggcacttt
    12721 ccgcggttgc gcacgagctg atattggttt ggttcaccca ggtagccacg ttttgtaatt
    12781 tggttgccac attacctaaa agaggagttg cttgctctag ggccaattgg aaggcaagac
    12841 cttgtgagtt gttcaatata ttcttggtaa aatcgacaaa ttctttacct ttgatgtgag
    12901 aaaacccacc taacgtggca tctatgccgc cacagcctgc ggacacttta ggccagctca
    12961 aacgcatgat ttgcagatta cgtacgctgc ttctagcgta taaattaccg ccagtataaa
    13021 aaccagcacg ctgcgcttgg taagcatgcg gctctgttac attcgcgccc atattaagag
    13081 ctttaaaata accagttaaa tcctcaccta ttgatttgct gtacacttgt ccgatgccaa
    13141 gtgcaccgat taacccacaa gacagaagaa tttttttcat aaaggcacct ctttgtcatg
    13201 tatctggcta atttttagta accgattggt tagttcgcct tcatcaatag tgccttcagt
    13261 aacaagactc gccacttggc tctttgggtt aatcaaaaaa agagaaggat aaacactgac
    13321 ttgaaatacc ttcgtttgtc cttcattcat caccgcatca ggaaaggatg gtaaaaagcc
    13381 tccatcaaca gaaatagcaa ctacgctaaa gccatagtgt tgactcaagc gtttgagcgt
    13441 gggtgcaaag cgttgacaat gaggacaact cgaactgaaa aaaaagaaaa atccttgatg
    13501 tttggcaagg gcatgaagcg gtgcattatc aatatcggcg tataggcttg atgaaaataa
    13561 cgttcctaat attagaaaaa tagctaattt attgcgcatt gtaatctccc gattgaaaag
    13621 cgttgcttgc cattaaaatg ttttccttca attccgactg gctgactaag ccataagcaa
    13681 ctggcatggt ttttttagat actggattaa gagcaaaaac cgcaggaatg tgtttaacac
    13741 ccatctgagt tgcctgaccg gaatcaactc gtgagttagg taacatcggg gagatgcgat
    13801 tggtcatggc aattggaata acagtcatgt gataactgtt cgcaaaatca ctcactactt
    13861 gggccatgcg ctcgctaaat ggttcaccac cttcatagaa atacaacaaa ccagatgttt
    13921 gactaatttg agtaatcgcc ttttcctttt gcatgctgtt ttgctggttg tagatatcac
    13981 gtgctgcact actgctcgga ttaataagac tttcatcctg atcggggttt attagcagat
    14041 cgagcatcca tttctcagaa aacagattag cctgtgccac aatcaatcgt tgtaatcgtc
    14101 ttgcacgagc aatattttca ggagttggat ttaaaatagc ttgtgcgcga gcctgttgca
    14161 ctctattacc aatcaatttc catgttttat caggatcggc aggtttactt acatgtgaag
    14221 gtgccactga ctcggtttgt ggtttattct tgacttcctc tttgggatcc ttatgccaat
    14281 accaaccttc ctcatgctca ttaagataag gctgctcggc gtacaaaaaa ggactcacgc
    14341 taaccaacaa aacaaaaaac catctagcca ttgatgtctc cttgattgta aaattgatta
    14401 aggcgctggc ttattccatt ggcagtctgt tggtaatccg gttgtttttg tttatttttt
    14461 acctcctcat aaaattctga aaaatcaatg gcttcgaagt gaattaattg caattgctca
    14521 ggcgtaatac ccgagcaatt tgaatattta ttttggccaa aaccaatatg taactgatca
    14581 cgtcttcctt gtacttgaac catacgggct aatttagatt ggaagacaca gtaagtctgg
    14641 tgatgatcga cacagaccga gccaataggg agttttttgc gtttaaaaca atattcaccc
    14701 gtaggtacta ccagtttgtt ttctcgagct tttcccagtt ttttctcagc atcactacac
    14761 tgtgccagct caagatccag accccacccc ttatcactgc aacaattttt aaaatggagc
    14821 attgttgttg aacattcaag caattgacct ttgaaaataa aattggcatt gccatcaaag
    14881 tcttttgagg catccgaggc ggcggataag gtggacattg cttttttaaa atcgtcttca
    14941 ttggctgggg ttagttcatg tttcgcgcaa ttaccatcca aacaaaaagc atcctcgcca
    15001 cacaacagtt ggttgtcagt acattggttt atcgggcatt gatacgtttg ctgatgggtt
    15061 gtgcaaagtc ctgcttcttc tttcacacaa gttgaagcag tctgctcaca tccttgatta
    15121 actaggttat cgcacgtatt ttgctcctga ctaccaccac aggtgtagga tgctctctct
    15181 ttccagcaag ggcgggtcaa tggaacatcg ccaataatcc gtgtttgatt gggctcagtg
    15241 catgttagag gctcagtaat atgacacaac ccctcttttt ctttgttttg caaatgttgg
    15301 cattggtcgt cccaatactc tttgctttca taaatttcta ccgtcatcga aacggagtga
    15361 acatagggca ctgtgcagcg accgaatcga caattgccaa cagacaatga tagggtggga
    15421 ttggcgcatg tagctgtttg ctcaacaacc actaatttct tttcatcaag gaaactgacc
    15481 ttcaccatgg cgcaattggc aggcaataga atcgctggtg ctggtgctaa atccttataa
    15541 atcgtatagc aaggagtttt gccttgttgg caggtatccg cttgtgccag attaactgag
    15601 actttaaaag gggtattcct catacctcct tttcgcaggt atagactgta actttctgtt
    15661 ttgtaggggg aaacatctaa ccttaagtgt cttgcacaat gcaatacacc taataccttg
    15721 ctagacaggc atgttttttc ctgccatgag taatggcatt ctttgggttt taaagagcag
    15781 gtggttttat cggtattttg accatgcata atcgcatctc cattttccgc gatttgattt
    15841 aacttttgca ttgattccga ggcaggattt acttgataca aggggcgatg attaaaactt
    15901 tcgcctactg cctttcctgc atcattctga gccacggcag tttgcgaatc ggcttcaaga
    15961 cctgtgcttg cctgcgtgac gccactataa taatgttctt gcggcagatt ggcttgatag
    16021 ccaggaaact ggctcagatc cagtgctttt aataaatcaa tggattgatt ggtatggctt
    16081 gttccggtct gaacgccttc ttgatacgcc tgattcaaat caagtgcaaa aacctgtgaa
    16141 caaaggatta aaaaaactaa aacgaagcct ttcattagat taattcccct cttaaccgaa
    16201 gaatcatttg atccaacacg gggtcttctg actgctcatc tcccctcatt ttttccaaag
    16261 catagtccag cgatacatca ccataaagac tgtcgtaact caccggtttg caatccatat
    16321 tggcaggaca agcaggggtg tctttcacat aaaccactgc aggaaccata gcgatatcaa
    16381 aggttttaaa aagaatagga tcaagctgca tcccaattcc tgttttttta ctgagcgttt
    16441 gaatagccgt catcgtttct ttaaacgaat tatgaattag tccccgaatc acaggtgttg
    16501 ctccactttt tttacattgt aaaagccagg cttttagact tttttctggc attgaaaacg
    16561 ataagaacac gagtacctga ctgacttttt ttaagtgggt tacaatgggc tcctgtgttt
    16621 ttatgacttg atgcatctcg ccggtaaatt ctgcaatttg acccgatttt tctgaagtct
    16681 caagagactt actgatttgc tttatttcat ttgtaaaagc attggcgcga ttttttgtat
    16741 tcgcctcaat agacgcaata taggcttcat cttctgcaag tgcaggaaat gacaacaaaa
    16801 ataatgcaac caagaaagaa gtgttcattc gtattgtttt catcacatct cctaatcgtt
    16861 aaaaggcgca acaattgcgt ttgcgccaga tcagataccc aaaatcctcg cccttaaacg
    16921 gatattcgtg ccctgcctcc catagggtcg tagcacgccc aaggggataa caggcattag
    16981 tagtcggtaa aggattgacc atttgatagc ggtaacgtga tttgggcaaa ataggggaat
    17041 aatgagtatg acaaatggat tttgcgctgc ttgtcgaatc gtcaagtcct actgtgtctt
    17101 ccaatagtcc ctggcggtgc agtttaaaat tcatacgctc agacagtaag actgatgcct
    17161 gaaccccgcc aacatgttct tgcacatgcc ccgttaaagg gtacatgctt ccctgagctc
    17221 ctgcgcacca aaagagtttg tcaatggcac tgcccaataa agcggtactt gaatcagcag
    17281 cgcaagccgc ttgtgcgggt aaactaccaa acaataccgc ctcaggatta atgacgaaac
    17341 ccagctcatc gtcattccac atcacatcca attcactcgg ataagcaatg tcaaagtctc
    17401 ctttttcaac acaaatccca tcagttaaca cattcaacca atacatcaat gggaacttgt
    17461 accagtgcac gtggtaaaat gattgattgg ataaattact atctgtttcc accgcacctt
    17521 tgcggtaata cttgccaaaa tttaaagaaa ttccccctaa attaactaag caaaatgggg
    17581 ttctggttac atccactaag ttaacaggct cccaataacc cattgttaat cccaatctcg
    17641 gtataggatt tcctgggcat tcacaaacag gcagtttggg atttttagtg tctggcaaat
    17701 gattggagga aacgagatta gcttgcccta aactgaacgg gaacaaacac gaccaacaga
    17761 catcagtaat gggatttaca aaacgaccat gacaagatcc tgcttgggtc aacccagaag
    17821 ctaaaaaagc aataagaata aaaaaggctt tcatacagtc acctcattaa tttgcaaccg
    17881 cttccctgct tgaaaaaccc gcgccggcaa atactttatt tgaaatttgg caaccaaaaa
    17941 tccatttaaa tcaaaataaa tagcttgctt taattgattc gcaacttcct ttacagagcc
    18001 tgaggtgagg attaatttta ctttttggtg ctgcgttaat tcctgggtaa cccatgcaac
    18061 ctgtgcctca tcatccccgt caaaaaacag gagagtgcta ctcaagccca ctctatccag
    18121 ggggttaatt acagtgcctt gtttaacaat cacttggccg tgactgtcct tcacatcgta
    18181 aggcacagca agtgatggat taaaaaacca cgttctattg tcttctgctc tcggcaaacg
    18241 aataggtgtt gggcgtgcca catgttcttt tacccgtttt ttaaactcag attgtacggt
    18301 cttccattcc ccattaacct gcatcccctt cattttttgc tggatgtact ctaaaaaatc
    18361 tatttcagca atttcaaagg ttttgccaaa gacaccatag tctttagcct ctgcaaaagg
    18421 gagatagagc atgcaaataa tcaacagaag ctttttcacg atttatgctc caatttgatc
    18481 atcgtcatga gggtggctgt ataatcagtg ctgcctttaa tgaccgcttc tcttggtaat
    18541 aacaccaacg atttggaatg ggacaagtct tgtaacgctg attccaaacg atgcgaaaat
    18601 gctttaattt ctgcttcttt ctcaaggttg gaatgatttt tttgcgcttc ctctttgata
    18661 aattgggagg tgattgacaa gatatcaatg gttgcaatat ctggccgcct caaagcacta
    18721 aaaaaaatcc cattaactaa gatactggtg atgagtgcgg tcaaaagaga tacttgagtt
    18781 gtcttaaaca tagccataca cctccagggc aactttggaa atggcatcag ccaatggcaa
    18841 acctgttgcc attaatcgtt tcaaggcctc gacttcagga ccacgtgtag tgtttaagac
    18901 gcgtgaaaat ggatcgacaa tcaaacgatg cactgttgaa ttctcttcac acatcattaa
    18961 aaactcagag tattcattag ttattttaag gcttcggatt aaacgctcgc cataagcatc
    19021 caactgatta ctcgttttta aagtgtcaat cgtacttttg gaggcaccta aaaagatttt
    19081 ataataggcg ttgtcgtaac acattttggc agttggattg ttttcataat catctaagcc
    19141 atgagtcact gtaataaaat taccaccgta tttaggcgca cgcctatagc cctcgttaac
    19201 aaagttagca ctgccacccg taccattgtc tgaaagtaat tcgtaggatt catcaatcaa
    19261 gcagcttttg ggaatgtgct ttggcgtgta atacatcgca ttggtgattt gatacattaa
    19321 gattttcaat acaatgcgct gcaaatcctt atttccatca agaccaccca actctaaaat
    19381 gactaaattt cttttaaaat cgagcgaaca aggcggttca aaataactgc cataacgacc
    19441 gtttttagta tagctataca acaggcgccc aatattttga gaaataggat cgttactact
    19501 ttctaagtga tgagcaataa gagtaatagt tgtagcattg tgatgttgtc gccacatcgc
    19561 ctcaatggct ttctcaatat gacttcgttc ttcatcactg acgttacctg atggtcttgc
    19621 catcatacct acaagttgta ttagctgatt gtgaacgtct ttaaatgcag tatcattttt
    19681 ggcttgtgca tcaattgtag aaaagaggtt taggctaatt tcagtgtcat gattaaaatc
    19741 aataagctgt ccgttcaaca tctcacaaga tttagcgtat gaaccgccca catcaatgat
    19801 ataaacaaag cccccctggg ataacagact ttgaatgtag tcattcatta aaaaggattt
    19861 tccttttcca ggaggagcta caatagcgac attatagttt ctgactacgt tgtcaaaaaa
    19921 ataaatagcg gtaaattgcc ctcgtctttg tggatagacc tgagcaaatc gtcctgcccc
    19981 tttccattcc ccctggatag gtaataagtt aaccgtatta aaactggtta aaggtcttac
    20041 ccgtccaaat gatttgtaat catcaaacat accttcagca ggtgcaaagg gcatggaggc
    20101 tagaagtgtt tgtaactgca aacctgttgg cgtgcgtaaa tcaaattggt taccacgaaa
    20161 tacatccttg gcacgtcgta catcgcgacg gacattggca ggtgttgtaa acaataaaac
    20221 gttataatag gaatcaacca aagaatcatc gccactgagc cgctctctta actcctcaat
    20281 ttctgcgtat tttttgttaa aatttttgac ccatttggca ataaaaggtt gtttggcatt
    20341 ttgcccttca tcagcatact tggcatctaa tgccgccatc accttgtctt tatctttaat
    20401 tcgtattgca aaactaaaaa gaaagggaca acttatttgc aaagaggtat taaacatttt
    20461 tccaaataaa tcacccagtt gccattgcac aggggattta ggatattgcc taacggacaa
    20521 gcatttaatc accctcgtat cttcttctcg ctcaatctct atttgattgg atttaacccg
    20581 catactcgct tcaatgtcag tcagttgaaa acgaatttca gtattggtat cgtaattggt
    20641 tttttcttca tacaagctgt cggttggaaa gagccactcc cgctttaagc taatcacttc
    20701 ttcaatagac agttctttac aaataatatt gactgacttt agattgttgc tgatgtcttc
    20761 gcgcaaacta ccaagcaaca agcgatcgtc ttctatggat ttgcgcggaa ttgaaataca
    20821 taaaaataaa cgaaaatcac gcaagacaaa attgctttct ttagttaatg agctgtacac
    20881 ccctttgcgg taaaattcag ctcgtcggtg tgccagggct tttaaaacct cactgccttg
    20941 attgcgttga tgttccattt catcaatcat gtggccaatt ttaggcgatg cccaaaacaa
    21001 agcatgaaaa caactttccg gtggcaaaat atccgttaat atactggtga gaatagtcac
    21061 tgtttcttca ctggcaccaa tcagtgtcga gcattcaaac atcatatgag atgcctgttt
    21121 gtaaggataa atatcagctt ctgaatcgta aacctcgtca ggtaaataag gtgctaagca
    21181 ataagtatcc aacagcgctt cgatttgctt tttatgatgc gcatcagtca tgccaagctc
    21241 ttctcgcaat gtttccaata gcgagcctgt tgcatgactt aaaatagtgc ttgttttttt
    21301 aactgcaaga cgcgcttttt ttatagttga caacataatg gctcccctta ttttaaagcc
    21361 aattgtgact gctgcatcca ctgtccttcc ttaacaacgc tgtacactcg attggcttga
    21421 tgataattgc catcagtgtc ttcataaggt gctatccaaa gaggcatcac cccttctcca
    21481 taacgcacag gctgaccagg tcgcccatcc atcaccaaag gatttcggct gggggttaca
    21541 gccagggatt tgcccttacc attcaccatg tcataaacct gatccatacg tttgcacgtt
    21601 cctccattgg gagctgggca atcaaagtcc tcattcaatt tggcgcaacc ggtcaaaaca
    21661 acacagggca acagtaataa agtagttaaa attttcatga tctatatatc cttagaaaga
    21721 gtggttttta tagaaaaatt ggttggcagc tttttcttca gcagtctcag actcggcagg
    21781 ggcttcaaaa gctactttgg aattttggct ttcttctggt tgttcagggg cattagtgtc
    21841 cgttccccca tcaagccaaa aacccttaag aaaagttaca tctactgtaa cgattggagg
    21901 caattgaata atcgggtggt acatttcagc acgcttgatg ttgtattgcg catacatttt
    21961 tgcagcgccc gatccgccac tagaaacagc cgcgagagga atttttcctg cttgaatggt
    22021 tgatacactt cctagaggcg aggtgctgta atcagtggaa tattgctggc caatattgcc
    22081 catgctttcc cagaagttcc cccaaaaagc ttttttcacc atcggttcat cacgccatac
    22141 cgcacgccca cgaattccgt ttttagaatc tgaaatgatg cctgtaacct ggatgtcgat
    22201 attggtgcca ttggaatgaa tgcaacttaa tcgttcggta cgaaactttc ctcgctcact
    22261 ggaaatatcg ccaattacag aagcacttac catacagcct ttaagtggtg ccctgtattt
    22321 attcggcatc actccatcat cgacaatttg aaatatcata atgtcgggat ttgcttggct
    22381 taaaactccc gcagatgcat ctgcaccttg taaaacaact gctttagcaa aggtgttgga
    22441 gaagacataa ttattgggtg ttttgattcg tggcttggat tttttattta acttggctgt
    22501 gtattgaata aatcccgaat ccatctccat ggagttgctc gctgccgctt catctgctcg
    22561 gggtaacaac ccattttgag gctcattgtt ttttaatgtc ccatctattg gggtagtacg
    22621 ctccatttgt agcttaacta tttgatgctt taattcagaa agtgtggatt tcatttcaga
    22681 tagttcatct ttttgcgagg taatgagctt gctttgcttg tctttttcat cacctaatgc
    22741 cttgaggttt ttatcaaaga gctctgattt tgcaagctca gctcgccagg cattttgtgt
    22801 tttctcaatc caatgggatt cattagtggc agtagatatc gggctggcta agctgtgttt
    22861 tttataagcg ggtttatcaa cctctctgga tttaactgtc ttgtttccca ataaggcaaa
    22921 gcacgcaatc accaacacta taacgccgat aacaatccct aaaaagatct gttgttgttt
    22981 tttaagttga atagacatgt tagctaatcc tgtacaatcg agtagtttca aacggcgcta
    23041 aatgggtttt agcaagcgcc acccctttgg ttccaggcca attaaaccag gattccttgg
    23101 catcaatagg ctggttggtt acgtttttta actcaaaaat ttcaccccgc atacgattgc
    23161 cattaactgt caataagcgt tttagttgtg ttttttgtgc tttatagact gaaggatttt
    23221 tgggtattgt ttgggtaaaa ccttctggca aatacccatt aacaagcgct cgtaaaaaac
    23281 caaccatcac cttttcataa cttgatgtat gtggagcgcg ggcacgaatt acaggatttc
    23341 tgtaggattt aaaaacgatt gtttgtgacg caatgggctt tggttctaca cgcacgttgt
    23401 aaaaaagacc tttttcagtc agcaaattga tagtaaaagc tcgtgtgtgg tatagaggcg
    23461 atggcaaaaa agtaagaata cccgtcgtat catcaattaa tttgcaattc gccttcccat
    23521 tgcatgcatc aaccagggtg ttgccaggtg cttttatttg ctgaatgcga tcatcctcta
    23581 acataagatt attgggttct ttaattgagg cattaatgaa aatatcttca taatttctga
    23641 ctgtttttat ttgagcacct tctgctgttg ctattaataa gcctaagctg aatatcattg
    23701 ccatttgttt catgattaca ccttctcaag attcgttagt ttcaacaggc catactcata
    23761 ggaataccta atcaaaaaag tctcccgtac atcagacatc aaatccgcgc caacaaaacg
    23821 cttcatctgt cccattactt ttacgctcaa gtgtttgata tcaatttcaa aggtcgatgg
    23881 ataaaacacg ctggataaat gttcgtgatt aatttgttcg acttccttaa ccaattgcgc
    23941 cctgatgtcc ccatacagtg agggagctac atagcctaag agcgcattaa attggtaagt
    24001 ggctgaactt gaagtgatat taaaacgaag ttgcgttaag aaattggtca tgtctcttaa
    24061 ataaccttcc gaaacacctg ttccagaaag tgtaaattct tgagatattt taggtgggat
    24121 gaaatgggtt tcatgtttgc tatgagcata atgcaataag accacaatgg taattgattg
    24181 catgaatgtc atagctagca gcacactact aatacctatg aataaaaccc taatcttttt
    24241 ttccttcatt aattctgatt ttttttgttc tattttcatc tgttacccca aaaacgctct
    24301 aatccatgac ggtggtgttg cccgtaactt aggtgataga gaaaagaacc aataaaaaag
    24361 gtggatataa aaatagggac cttcattacc cttcatgcgt ttcataagaa atagaaacgc
    24421 acaggttaga atcaatgagg taacaaaaaa accacaaaca gccccaaata cgtagagagt
    24481 tagtgctcct accatgacct catcgagtgt aaaaataatc aatcgataag gctcattaag
    24541 atattggggt gttttgtatt ttctcggatc ttccatctta aacccctgct agcattgcaa
    24601 cgacatttag aaaaatagcc atcgccccaa cgccgccaag cgttttaata tttcgagtaa
    24661 acatcaaggt aacaacagcc gctacagctt ctccgacata aagataggtt tttatcgaac
    24721 cattgtaggt atccttaact acttcttctg cagcggataa agcatccgct gcaaaggttc
    24781 cttgagccat tattagcaag caaataagta tgaaaatctt tccaatgtga gcattaatac
    24841 tgcttaacac gttacttctg aactgattta ataaagacat gttatccctc ctttttaatg
    24901 gtgattgagg attgattgct gcattgatgg gcaaaatttc tttgttggat cagccaaacc
    24961 atcgtgcttt tcagattgaa ttttttgata aatttcataa cggtggatgg ctactgaatt
    25021 gggtgcatta aacccaatgc gtacctgatt ccctttacaa catagaactg tgataaatat
    25081 atcttctcca ataacaatgc tttcaccgac tcttctagtt aaactcaaca tcttcttctc
    25141 ctttttagtt aactttcata tttaaaatgt gtttcatttt cagtaattgg gttttagcca
    25201 cttcagggtt attgtttttg ggagctgcat caggtacaaa aaatgaactt ctcttggaac
    25261 aatttttaca aaaatcaata aattggggta aggttggcgg tagttcctta ttttttcgac
    25321 ataaatcaat tgccatatca agaatgttat ccgcataggt aaatagtcct tgttgccatt
    25381 cacctttaac aaaaactaaa aactcatcgc ttttaaattg actacgccaa acctgtccgt
    25441 acatagctgc aaacctcaaa aataatcgat caatgcgctt gcgaccttcc tcggtaggcg
    25501 ctaaggtcga tgatgttgtc atcggtttct tcttcaatgc ctgatttaca tgactcccag
    25561 aagctgtctc taatgggttg tttcttataa tttgtttcat ggactgcatt atttgtctcc
    25621 tgacacgtat cactttgaat ttcatcttcc caacaatgtt gtgctaacca attggcagga
    25681 tatttccaag cagcaaccca ttggccttgg ctatgctgtt gttgataaaa ctggatttgt
    25741 tgttgtaaag ccgaataaat ttgtattggc aactcaggtg tgagattaag cgcttgaaat
    25801 gcttcccatg ctttttgctt tgattttttt agcgggtaca aatcccaaaa tttttcgaat
    25861 tgtgataata aaaaaatata ataattatct tcttttagag gtgtgtcggc ttttggagat
    25921 ttatctatgc cggcttttag tgggtctgtc tttgataagc cagtatttat aaggcgttgc
    25981 tgatttttag ctgggtctag ttgctgtgtc tggtttaggt ctagtttatt ttggacggaa
    26041 taacccattg tagctaattc gcatcttaaa attaatttta aaccttcgga ttgtggtgta
    26101 ataagccctg ctcgccccaa agcagataag gcacggcgta tttgcatgcg tgaatagctc
    26161 ccgcttttaa tgccttgatg cggctcaata taaagttgct ctgctatcga ttgatagctg
    26221 attccgcgct ttatgccgac taatcctgtt ttgacatcca tatatggcct tatccctctt
    26281 aggtaagcca gttgctgaag gtgtggcatc ccacataaag cagcaagttc tttgtcatta
    26341 attaaaaa
//
LOCUS       DASLOE010000026        26040 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.026, whole genome shotgun sequence.
ACCESSION   DASLOE010000026 DASLOE010000000
VERSION     DASLOE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 26040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26040
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.026"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..2182
                     /locus_tag="U6309_000883"
     CDS             <1..2182
                     /locus_tag="U6309_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN5504336.1"
                     /translation="FKNQTNKSALNSNLKTNTDGTNPIDITSQFLTEQINDKSKNTSD
                     YVYAIYRYMENIIAQIPVSVYWMNKNHVYMGCSNNMVKLLNLNSRYDIVGKTYEDLYH
                     EKSTSYYKKADKIVMDKGIPLSLEEPFYCPDGTTKEIYLSNKVPLRDLEGKIIGMLGV
                     SVDITDRKKMELELSKAKEAAEEANNVKTEFIANMSHDLRTPLSGVVGMAELMKEQVT
                     DPVQVQYAEWIFDCSQQLLSLLDGILDIVSLGNVKEDELKITTFDINECIAELVHLEM
                     PSIKLKQLDLIVNIDKKVPRLIKTDRIKLHRIILNLLGNAIKFTDSGYIAIDVKLLEL
                     NNESGLIYFAISDTGIGIPENQQTKIFDRFHRIIPSSIGKYKGHGVGLHIVQSYVNLL
                     GGEIQVNSEPGVGTTFYFELPIQIEKENEVTKGHYKLEKNIIPVPKKEKILDKNSKNE
                     FLYNSPNILILEDNLVARRTIESLATQEKCRFVSVAKGKEAIRLAKTNEFDLIITDIG
                     LPDIEGHVVAKLIREWELSQSKPPIPIIGLTAHIKEKSKEICLKSGMDDVFNKPISRQ
                     LLQYILSRFIPSKNKNPTSCSSQKTASSEQIIEADLPESESNFFNLEAYPLLDIHNAV
                     SIMGSLKLVREILPLMIGEETQKDVESIEKAFHIKDWETVERIAHKMKGGAANCGAVR
                     MKYACQYLERYQKMGYTKLLEPLYQQLIKVVEDTQKNIHEWLKH"
     gene            2354..3262
                     /locus_tag="U6309_000884"
     CDS             2354..3262
                     /locus_tag="U6309_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN5504337.1"
                     /translation="MSDEISKEYFDETLGIHQEYLGILNCLPDIVYWVDKDCLLKGCN
                     QNFIKLLGLKKIKDLKGTPYEQMAKYTGWPEKNIEAFKLDDMKVLFSGEPQWDVEEEP
                     LFNKDKEVSYYRTRRVPLFDKDNKVYGLVVVLTDITKFKKMEEQLQKVATKEKIKPSP
                     AKRIEEPKILMVEDNFVAQKVEEALLKELQCQVDIADTGDKALNLFDPGKYDMVFMDI
                     GLEDTSGYLVAKKIRQKEQNTNYHVPIIALTSYQADVVKYDVEDYFMDGVLTKPLTSE
                     QADQLIKHFVHKEDIMVSGLKSAREN"
     gene            3544..4305
                     /locus_tag="U6309_000885"
     CDS             3544..4305
                     /locus_tag="U6309_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5504338.1"
                     /translation="MIWDPERYVLGNYIQNKINDVFLKTIHSKLSGNILDIGCGDGHY
                     TSLLASKIKHGQILGIDSSEQMIMHANQQWARTGLSFEVHNIEEFHQPQSFDLALSFW
                     CLHWTNIHISFPNIFHLLKREGKLYAVMSSFSDHSILQTWKELAKQNLYRDLTEQYIS
                     PINEQYFYGVINLLNRLPFKRIKLDLKTCRVHLPHIDYYKNLLLTMPFIKRVPSEITD
                     TLVEDMLKAFQTICDRKYGGQLYYETRPIFLKAIK"
     gene            4330..5364
                     /locus_tag="U6309_000886"
     CDS             4330..5364
                     /locus_tag="U6309_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEN5504339.1"
                     /translation="MHLNFNTTHGMNIPMVIASQDNLKASPTPLLHFISEYHDEIIQK
                     LNQYGAIVLRGFSCQEENCFSKAIELCALGTRCSTSDYDLPRTVLANEIYTSSDLPAH
                     IPLPLHHEKPRSPKPPNHIYFCCIIPPQEGGGTIFANAEEIWIDMPQDIQNKILEYGV
                     QYKQFFHGQSVKYRVLRKILGNHCARSWVDYFGTEDKTQIEQNLTQKQVVWDWINHGN
                     DLIILNYLPGALKHPLTDKIAWFNSSAYLNYYSNLNYGELKNLRSFKYWASRYLILKD
                     MLPMICHYGNGQAFSAKEISEINQVIQRHTRVFHWQKGDFMIVDNFTFMHGKQAHVGE
                     RLLYSCMTAY"
     gene            5622..16961
                     /locus_tag="U6309_000887"
     CDS             5622..16961
                     /locus_tag="U6309_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN5504340.1"
                     /translation="MVNVTMKNKTKDLLNEPIAIIGMNCQFPGIDSDIEDVSAFYDML
                     MKGQTPIKDVPENRWNIEEYYSADRKKADKIISKKGGFLDNTHLFDAAYFKISSAEAK
                     QIDPQQRLFLEVAIRALNDANIPLDSLKDSNSGVYCGISTHEYSQLNYKDHIKFNAYT
                     PIGIANSAAAGRLCHFLNLKGPSMAVDTACSSSFTALYLAATALRNQQCDMAIVGGVH
                     LSLCPESFIGLTKANMLSATGQCSSFDSKADGFVRSEGCGVVIVKRLSDAIRDNNKIY
                     ALIKSMVINQNGDGTGLAAPSTNAQIAMHQAALKHAHLTAGEIDYIETHGTGTTVGDP
                     VEFNAIQSIHQGHHSAQKPLIVGALKSNIGHTISSSGIASLIKVLCSLQNEAIPPNLH
                     YSNPNSLIDPESIPALLPVGAIPFPKLKNKKRYIQVSNFSFTGTNVSVILEEAPELES
                     HESKQDNNEPKCFVASANSELSLKQLMSRYEHYLKNSSSTLGDICHTLINCRDHYKFR
                     CAIIVNDKKELIKKIESKDYEIRKVVLQNNPRTVLNDAKAIYEAYLSGINISTDDENT
                     AYNKVDLPLYCFDRKPYWHEVRKQVKNDHWLDSLYQQPIEQQVATIKTELASQIKDLL
                     KKDNIDPHQDLETLGLTTSLLESLDQILQDMFSPRYKAPSLQSLRYLTLDKLARHLQK
                     MIMPPVVYRQPSINVLNVEPIAIIGMSCRFPKAANVDEFLSLLEKGESGMIDIPLERW
                     DNEKFYDSDVDALGKLYIKQLGFIDHVNHFDAEFFNISPREAKLMSPQLRVFMETSYH
                     ALEDANLSLDAVKDTNTGVFVGVGTNEYPRVLAYQGVTLEDLNIYFATGNVLNALAGR
                     VAYSFDFHGPIQAIDTACSSSMTAIHNACLSLQSGDCNMALAGGVNVILSPDSNITLS
                     KARMLSPDSRCKTFSEDADGYGRSEGCGVVVLKRLSDAVKDNDNILAVIKGTSINSDG
                     KSGGFTVPNGAAQEEVILSALAKANLSPGDIDFVEAHGTGTPLADPIEVNTLTKIFSE
                     HHNKENPLYISSVKTNIGHSESASGVASVIKTVLSLKTKTIFKHLNFKKLNPAIQLIN
                     TVIPLDKTDWAKKQGLRCAGVSSFGFSGANAHAIIQEAPEDKKEARALPEESLLVLSA
                     KSKTSLELLLASYQNYLSNTREEFADICYTAATCRSHFMYRVAIKAQNAAQAANIIKN
                     NGYTICHITKEKEWTQQPDTLAELQTAYLDGFPINWSEFYRSLGHHFAKIKLPLYEFE
                     RIEHWFGDKNKLKDAPLPKDWCFQIQWQNEPCDKSNRKLRGNRWLLIGPRHLADGFKA
                     QGLEIIYEDEEYSRDKLEGIIFAESLHRAPPDIEANIDFQKKTLKKLLNLIKELDNQS
                     IDLQLLILTSNGIAELANDKHPVNISNSPLIGFCKTLALELPQFKTILIDMDKIEEDK
                     YPTQVIEEINYNHGSRYEHIVAYRGGERLVSRLKKTPLEDRRRLLHGVKGRYLITGGC
                     GGLGLVTAQSLLSSGARELILTSRNIDKPRIKEAIYKIKSHYPGANIRVVSLDITDRG
                     SLKNLLSELNADGQLKGIIHAAGAAIKAPLLEHQDEDVDYLFSAKVKGGWYLHELSMD
                     CNLDFFVVYSSVSSVFGSNKESVYSGTNSFLDALIAERQRMGLPGTAVQWGPWGEVGM
                     AQKRSRDEGLKQALISNAQGHVIIKCLINDEFKHATIISPEYLKFMLDFVPKPLPGFY
                     GQLDEELEKIQQHHQSPAKNLSPWLNQYLEISEEERFKACKAMVSDICTGILELTENE
                     DLNEDEGFFELGFDSLMITELASELKKKLEPFLKVTVTIGFNYPSISKLAKYIESELD
                     KNLVKIQSPKSALKSIDDGIAIIGMSCLLPNAPDIATFEKLLEEGLSGIKDIPNDRWD
                     NSQYYDPDMEAPGKSYVTKLGLIENIKYFDAAFFGISPREAKLMEPQQRVFLECCYKA
                     LENANYPSESLRGSLTGVFAGVGPNEYYAQLEKSGFSNEELSAYSITGNVLNLIPGRV
                     AYTFDFKGPSISVDTACSSSLVAIHYACQSLKNKEIDYALAGGVNILLMPESNVTLCK
                     AKALSPDGQCKTFDEKADGYVRGEGCGVILLKRLSDALRDNDHILAVIKGSAVNNDGK
                     TAGLTVPNGKSQEEVMKKALGQTELSRTDISYIEAHGTGTPLGDPIEVDAINKVYGHQ
                     RNKENPLYLGTVKTNIGHLESASGVAGVIKTVISLQRKTIYKNLNFNKLNPNIKLDGT
                     QLALEKTNWNTNTKLNYAAVNAFGFSGTNAHVILAEFPKEEKPKTSKPPQKHLLVISA
                     HSKTALDNLTQRYQQYLETTEHHFGDICFTAATCREHYHYRLALTAENAVEASRMLGA
                     GQFALSYHENITLDLQNDRALNSLLMDYLQGKPVDWKSYYQSLKGEFTKVLLPNYTFD
                     RSEYWLDKKNKDSMATHKGEQHPLLGQMLAMPGNEYLFHQKLDLENLAYIKQHSVFEK
                     VIFPATAYIESGLAAAKSIFQSSAFQIEKFNIERPLHPKQGQEFQLQVKPKMDGQYKI
                     NLFAKQENDWQTFCEMEIHAKPTAARESIHIDVLKSSFGDKVELSDIYEQFQKSSLFY
                     GDEFRVLQEGYVRANSVLAKVTLTKASQDQDYYYHPVLLDGAMQSILLLSVKNDDNKN
                     FNAENVTYIPYAFIRMTVFQEAPRSLWVHLTKQNTEDASDLCVNMNLYEQSGLLIAQI
                     AGLKLRRVTRSHFVSYDAALNHLYYTEWSPVPLKTIPQQKIPDFLAITKNPGKAKEIL
                     DGLNYHLVERIDDCTNIENSNVVFLYEQGQFHDLFHCCQILFRLRPKRFILVTEHAYA
                     IQDHDQVNPYHTMACSFWKSFRNEYEPNKNYAIDLDSRSRLATALMYLLNTDTHDTQI
                     AVRESLYLPRLKKKQLVSNSAPERLIDGNATYLITGGTGGLAKPLMEYLMRRGARHIA
                     ITSRAHYQDDIKALIDKAKQNQIDIKHYMVDAGNYQKMEQVIGEIQQSPNRLKGVFHL
                     AGLIQDGLIVNLNDEAMQKVISAKMDGALILHQLTQNIPLEWFVMFSSSASLLGARGQ
                     ANYAAANGFLDGLAHLRRQQGLPAISINWGPFHTTGMAANLTHTLQHYGFLPLDKDNI
                     DILDVLLPSQLPQISPCPMNWDVYCKYTPKQTELSALVKAKPLPDQSFLNALRQHSKE
                     EGISILSQALREIAADVLALDDSEHISNHHDLFSMGLDSLMSMDIRNRIYDKLQCQTL
                     SLPIEYFINTPTINKIARHISDELDHVFDSHSDYHPTENPSEREIALCDFQYVFWVLN
                     KLDYHFNIGTQIQLHGRINKDYVQRAFEFVVNQNSVFWIHFNKDKPTQKLHKVGQFEL
                     IYQDISLNHETKALNHEFQNNILRAIPLTKQPLIRVYLYKINNDLHELHIVIPHIIVD
                     DASCEMVFCQFKNNYEKLTLGKELVSTPEKGSYFNYVKQNNNHYQKNLKNKIDFWQNY
                     NKGFKMLSFGNRYHLSDTSKQTKHLFHYPIDPQHAEQFINWHKAQNINVSTGLIAACQ
                     IVFYKLSRQNKIPIILIHSGREGGEYKSTLGLFSEYKRINLTLNENSQFIDCIHSIEE
                     QLLKTAPYQKCSHLIKDSGLKGSSLSFGQYLTLALNKMFMLKHFKESKLHSIIIDYYL
                     KYLSRLLSFKKNISMKQRLNQLFKLDMSLQKPERLRVLVSVTPSFFTKERPDMSFANL
                     DYSYPNHFGCMDRPIGNRTLWIYFSKNQDGEYQISINAPLTIECKNKIGSGLSQFITT
                     FVENNNCNIAELIQAMEDSSIAS"
     gene            complement(16964..17365)
                     /locus_tag="U6309_000888"
     CDS             complement(16964..17365)
                     /locus_tag="U6309_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN5504341.1"
                     /translation="MKSLRMAGVFFLFLFSLVSIHVYAAPNTGASNAEMKDVCQLAGG
                     TFTSTIEGNWACCWDNWGCYGCVQGVCKMKCNTQRCRRANGQSAVLQPGEVKIKGKFG
                     TDIVVPKVSTQPSSSNPKQIKNNNTTVNQAQ"
     gene            complement(17596..18549)
                     /locus_tag="U6309_000889"
     CDS             complement(17596..18549)
                     /locus_tag="U6309_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEN5504342.1"
                     /translation="MTSISEWWMWLGFLAFVLIMLFVDLFVFGGRKAHRVSTKEALSW
                     TILWFTLAIIFNFLLWWYLVENFTFSVANEKALEFFTGYLIEKSLSFDNIFVFLILFE
                     YFAIPAKYQHRVLIYGVIGAIVMRLILILFGIWIVNQFHWILYIFGFFLMITGIKMFI
                     FADKKPELAKNPILHWMRSHLRITQELHGERFFLRKNQLVYVTPLFMVLVLIEISDLI
                     FAVDSIPAIFAITNDPFIVFTSNIFAILGLRALYFLLVNMHKRFYFLKYGLAFILVFV
                     GFKMLIAPWFKIPIFVALGIVMATLVFCILFSIWHRKNEVT"
     gene            complement(18665..20527)
                     /locus_tag="U6309_000890"
     CDS             complement(18665..20527)
                     /locus_tag="U6309_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HEN5504343.1"
                     /translation="MSKHLFYGVFLILLSMKAYPQDAVSRDAAMRTESIEKKGIAYHN
                     LIDCNYKISPEISHIDSDIILNWAEYAAILSFNFNFASIDNQLNQLHSCYTEKGWGQM
                     QNALHKSNNIEIIKAEKLKVKGYKDGEAKIIEALDNRWKIILPLKVVYENDKKRVTHF
                     LNAYLTIGWKNKDSLGIMQMIAIPRLAPLAQKVTPADEVIKSMGIILAERKKASLDSA
                     RKITDSFFVPLFSNHGERSKRNLALSNRAAQRVAIVQPMPNLNPTYNLVGRQSFDVPE
                     SAKTPYLAVSQQRSLVNDSDSNKSTLSDFGNKGRKLYTNWLSQIGLHSSKLQLNSLIT
                     RINPLPLWHAFSQYQQSIRDKSDNIEAINKQKPILSAQIEGEPQFIEVKENQWDIKLP
                     IKVVYQKDDEKITQRLDVNLALDQKMINNLMVKTKSVDSNKVLQSLKSILASSSLQLS
                     PQLFLGSQNIQPKQPEQSKPKGTVSCRYKIPEEIIKTDKNIILQWAEHAATQSFAFDF
                     TSIESQLNKLQSCYTENGWIEFNSAMMKSGNIEAIKMQRLTMTSEVNGVAEFIETREN
                     HWIIALPLKVTYKNDTMRVSQLLNIQLTVGRKTNGEFGVIQLIATLAPSSPPPS"
     gene            complement(20609..20785)
                     /locus_tag="U6309_000891"
     CDS             complement(20609..20785)
                     /locus_tag="U6309_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504344.1"
                     /translation="MDKNYIRSSRYPRPVCAEEMRKHRLEALARNIKFHTRLRDGINA
                     SSARTIQDSDRFYP"
     gene            21064..21564
                     /locus_tag="U6309_000892"
     CDS             21064..21564
                     /locus_tag="U6309_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEN5504345.1"
                     /translation="MNTTSLSLTPLLRHSVGFERFNDLFESMRNADDSTYAYPAYDIE
                     KHGEDSYTITMAVPGFQESDLNIMVQNDQLRVSGRIQEKETKESEYLHRGIVTRAFEQ
                     TFRLADHMKVTGAEIKNGLLSISLIREIPEEAKPRIIPIKSISDQESNKKSKTIEPES
                     KKKISN"
     gene            21746..23266
                     /locus_tag="U6309_000893"
     CDS             21746..23266
                     /locus_tag="U6309_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEN5504346.1"
                     /translation="MKRGLFVHLKHDLPASIVVFFVALPLCLGIALASHVPLFSGIIA
                     GVIGGVVVGFISGSALGVSGPAAGLVAIVITSVEALGSWEAFLLAGVIAGILQLIAGF
                     LRGGIIAYYFPSSVIKGMLTGIGIIIVLKQIPHLLGHDVTPLLLSMSDGELDINDIRE
                     ALDDINPEVVIISIVSLLTLIFWEKVLVKKHKLFEMIQGPLVVVILGIALNLFYEHRL
                     TSFSLDPSELVQLPKFNKASELFNYLTLPDFSLITDLKIYKIAILIALIASLETLLCV
                     EAVDKLDPYKRVTSTDRELKAQGVGNILSCLIGGLPITQVIVRSSANVAFGAKSKLST
                     ILHGLLLFICVISIPEILNEIPLASLASILTVIGYKLAKPALFKQMYKMGWEQFVPFL
                     ITIVGIIFSDLLFGITLGFSVAIFIILRHHFLNSHDIIKIRGKNKTQYCLKLAEEVTF
                     LNKGSIINELKNIPEGAEVIIDGSRSKIIDHDIKEVFQNFILNSKTKNIQVKLIGI"
     gene            23270..23896
                     /locus_tag="U6309_000894"
     CDS             23270..23896
                     /locus_tag="U6309_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842179.1"
                     /note="macrophage inducible 5; Mig-5; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEN5504347.1"
                     /translation="MWTLTKEQQQAITPEKAIELLKEGNKRFVSNLKLNRNLLQQVNE
                     TSQGQFPFAVILSCMDSRTPAELIFDQGLGDIFSIRVAGNILNDDILGSIEFACQVVG
                     VKLIAVVGHTQCGAIKGACDGVKLGNLTNLLNKINPVIQEAKKLDAKHDVHSPEFLNC
                     VTSLNVKHTMNEITQRSEIVHQLVNEKRIAIVGGLYQLETGEVQFFDE"
     gene            complement(24014..24307)
                     /locus_tag="U6309_000895"
     CDS             complement(24014..24307)
                     /locus_tag="U6309_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504348.1"
                     /translation="MNHSVKKKMLISVLYSLRHLIALLVMLVGTYLIKIVTVLLYIPS
                     DYSTLSLLSLCRVLWLSNEFFLRFILVVNFIIKPLFLYFGILFWFYYLNKKYH"
     gene            complement(24338..25309)
                     /locus_tag="U6309_000896"
     CDS             complement(24338..25309)
                     /locus_tag="U6309_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN5504349.1"
                     /translation="MSLRILSLSDVKHSITMHQAISALESAFIQLANNTVKLPLRTGV
                     TIEEENGLMLTMPGYLAQEKSLGLKVVSIFPENLAKNKPTITGFIMLLDATTGEAKVL
                     MDAAYLTSLRTGAVSGLATKYFARDNATQVAIIGSGAQAKTQLEAVASVRDIQQVFVW
                     SRNIKNAEKFANEFEDQFDMTICSSVAMAVKDADIICTATNSTEPMIHFGDISPHVHI
                     NAIGSHHPSMKEISHDVLSRAVVIADQLDAVLAESGEIISAVEHNHLKKESIIEIGQW
                     LLQKNTDCKDKLTVFKSVGLAIQDLSVANVVYQNALKKNLGELFSIS"
     gene            complement(25364..25810)
                     /locus_tag="U6309_000897"
     CDS             complement(25364..25810)
                     /locus_tag="U6309_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEN5504350.1"
                     /translation="MKSIANKENEVAIIHKLIEEYANAARDKDIDKIMSHYAPDIRSF
                     DAVSQLQFKGANDYRKHWQTCLSFCPGPTVFEVHELETLVDNNLAVSYYLTYCGGTNE
                     KGEMQGGWMRGTMVHCKMNGKWKIMHEHYSVPFDMKTGNTLFDLKP"
BASE COUNT         8567 a         5001 c         4836 g         7636 t
ORIGIN      
        1 ctttaaaaat caaaccaata aatccgcatt gaatagcaat ctcaaaacaa acacagatgg
       61 taccaaccct atagacatca caagtcaatt tttaacggaa caaataaatg ataaaagtaa
      121 aaatacttca gattatgtat atgctattta tcgctacatg gaaaatatta tcgcacagat
      181 tccagtaagt gtttattgga tgaataaaaa tcatgtgtac atggggtgta gcaataatat
      241 ggttaaatta ttaaatctca attcaaggta cgatattgtt ggtaaaactt acgaggatct
      301 atatcacgaa aaatcaacct catactataa aaaagcagat aaaatcgtta tggataaagg
      361 tatcccatta agtctggaag agccattcta ttgtcccgat ggaactacta aagagattta
      421 tttgtcgaat aaagtccctc ttcgtgacct ggagggtaaa attataggta tgctcggggt
      481 atcagtagat attactgata gaaaaaaaat ggagttggaa cttagtaaag ccaaagaagc
      541 tgctgaggaa gcaaacaatg taaagactga attcatagcc aatatgagcc atgatctcag
      601 aacccctcta agtggtgttg tgggtatggc tgaacttatg aaagagcaag taactgatcc
      661 tgtacaagta caatatgcgg agtggatttt tgattgcagc caacaattat tgagtttatt
      721 agatggcata cttgacatcg tatccttggg aaatgttaaa gaagatgaat taaaaataac
      781 aacctttgac atcaacgaat gtattgcaga attagttcat cttgaaatgc cgtccattaa
      841 actgaaacaa cttgatttga tcgtcaatat tgataaaaag gttcctcgat taataaaaac
      901 cgaccgtata aaacttcatc gaattattct caatctttta ggtaatgcca ttaaatttac
      961 cgattctggt tatattgcaa ttgatgtgaa attacttgag ctaaataatg agtcaggatt
     1021 aatttatttt gcaatatctg atactggcat tggtatcccc gaaaatcaac aaacgaaaat
     1081 atttgacaga tttcatcgta ttatcccgtc cagcatagga aaatataaag gtcatggcgt
     1141 tggtttacat attgttcaat cctatgtaaa cctgctaggc ggagaaatac aggtaaacag
     1201 tgaaccggga gtaggaacta ccttttattt tgaactccct attcaaattg aaaaagaaaa
     1261 tgaagtgaca aaaggtcatt ataagcttga aaaaaatata atacctgtac ctaaaaaaga
     1321 aaaaatttta gataaaaact caaaaaatga gtttttgtat aactcaccta atattctcat
     1381 cctggaagat aatctggtag ccagacgtac aatagaaagc ttggcgactc aagaaaaatg
     1441 tcgttttgtt tctgtagcaa aaggtaaaga agcgatacgg cttgctaaaa ccaatgaatt
     1501 tgatctgata atcacagata ttgggttacc agatattgaa ggacatgtgg tagcaaaatt
     1561 aattagagaa tgggagttat ctcaatctaa gccaccgata ccaataatag ggttaactgc
     1621 acatatcaaa gaaaaatcca aagaaatatg cttgaaatca ggtatggatg atgtattcaa
     1681 caaaccaatc agtcggcagc ttttacaata tatactgagt cgattcattc catcaaaaaa
     1741 taaaaaccca acttcatgct cttctcaaaa gacagcatca tcagagcaaa taatagaggc
     1801 agatttgcca gaaagtgaaa gtaatttttt taacttggaa gcctatcctt tactcgatat
     1861 tcacaatgct gtttccatca tgggtagtct caaattggtt cgggagatat tgccacttat
     1921 gataggtgaa gaaacccaaa aagatgttga atccattgaa aaagcttttc atataaaaga
     1981 ttgggaaacg gtagagagaa tcgctcataa aatgaagggt ggtgctgcga attgcggtgc
     2041 tgtacgtatg aaatatgctt gccaatattt agagcgctat cagaagatgg gatataccaa
     2101 gcttcttgag cctctttacc aacaacttat aaaggtcgtg gaagataccc aaaagaatat
     2161 tcatgaatgg ctaaaacatt gactctcact agaaacatca atggtattca ataattaagc
     2221 ccccagaaaa aacctcaaat ataatattta tcaatcaatt tgaatattat aaatacacat
     2281 taattaactt tgctataatt aatttaaata acaccaaatt ggattcggtg taacctttgg
     2341 gaggaagcag taaatgtctg atgaaataag taaagaatat tttgatgaga ctctcggcat
     2401 ccatcaagaa tacctaggta tccttaattg cctaccagat atcgtttact gggtggacaa
     2461 agattgttta ttaaaaggtt gtaatcaaaa cttcataaaa ttacttggtt taaaaaaaat
     2521 aaaagattta aaaggaactc cttatgaaca aatggcaaaa tacactgggt ggccagaaaa
     2581 aaatattgaa gcatttaaac tggatgacat gaaagtgctc ttttcaggag aaccccaatg
     2641 ggatgtagaa gaggagcctt tatttaacaa ggataaggaa gtgagttatt accgaactcg
     2701 tcgcgtacca ttatttgata aagacaataa agtatatgga ttggtagttg tactaactga
     2761 catcaccaaa tttaaaaaaa tggaagagca attacagaaa gtagctacaa aagaaaagat
     2821 aaaacctagc ccagccaaac gaatagaaga accaaaaata ttaatggtcg aagacaattt
     2881 tgttgcacaa aaagttgaag aggcattgct taaggaactg caatgccaag ttgatatcgc
     2941 ggacacggga gataaagcat taaatctttt cgacccgggg aaatatgata tggtctttat
     3001 ggatattggt ctcgaagata cttctggcta tttggtagca aaaaaaattc gccaaaagga
     3061 acaaaacacc aattatcatg tgccaattat tgccttaaca tcttaccagg ctgatgtggt
     3121 gaaatacgat gttgaggatt attttatgga tggagtgctc actaaacctt taacaagcga
     3181 acaagcagac cagttaatca aacactttgt tcataaagaa gatattatgg tttcaggctt
     3241 aaaatcagca agagaaaatt aagatcaact tgcttagctg acaatatctg atttttagtt
     3301 tgttcgcatg aaacaaaatg aaacattgcc aacgcctggt aaatagagaa agtgttaaat
     3361 tggcacacca gtgcactaca ggattaaagt acaacctcgt cggcgagggc tgcatcctca
     3421 taaaaacaag acacatcccg cataatgtgt gagaatagat ttcagccatg ccaaagatat
     3481 ttgtaatcgc tggtttcttg ggtatatctt cccttacaat gattattgat actggagaat
     3541 gaagtgatat gggatcctga acgttatgtg cttggaaact atattcaaaa taaaatcaac
     3601 gatgtatttc taaaaacaat tcacagcaag ctgtctggaa atattttaga tattggttgc
     3661 ggagatgggc actataccag cttgttggcg agcaagataa aacatggcca aattctggga
     3721 attgatagct ctgaacaaat gataatgcac gccaatcaac aatgggcacg tacggggcta
     3781 tcttttgaag ttcataacat tgaggaattt caccaaccgc aatcttttga tttggcactc
     3841 tcattttggt gtctacattg gacgaatatc catatttctt ttccaaatat ttttcatctt
     3901 ctaaaaaggg aaggcaaatt gtatgctgtc atgtcttcct tttccgatca cagcattttg
     3961 caaacctgga aagaattggc caaacaaaac ctgtataggg atttaacaga acaatacatc
     4021 tccccaatca atgaacagta tttttatggt gtgattaatc ttctcaacag attgcctttt
     4081 aaacgaatta aacttgattt aaaaacttgc cgtgttcatt tgccgcacat tgattattat
     4141 aagaatttac ttctaaccat gcctttcata aaaagagttc cttctgaaat aacagataca
     4201 ctggttgagg acatgttaaa agcatttcaa actatctgtg acagaaaata cggtggtcaa
     4261 ttatattatg aaactcgccc catatttttg aaggctatca aataactcaa ggtgttagga
     4321 ggcaataccg tgcatttaaa ttttaatacg actcatggca tgaacatacc tatggtcatt
     4381 gcaagccagg ataatttaaa agcaagccct acccctttgt tacattttat ttctgagtat
     4441 catgatgaga taatccaaaa gctcaatcag tatggagcaa tagtattaag aggattttcc
     4501 tgtcaagaag agaactgctt ttcgaaagca atcgaattat gtgccttagg cacgcgatgt
     4561 tcgacaagcg attatgactt gccaagaact gtactggcaa atgaaattta cacatcaagt
     4621 gacctgccgg ctcatattcc cttaccgctg catcacgaaa aaccccgatc accaaaacca
     4681 cctaatcaca tctatttttg ctgcatcatt ccgcctcagg aaggcggtgg aacaatcttt
     4741 gccaatgctg aagaaatatg gatagatatg ccccaagaca ttcagaacaa gattcttgaa
     4801 tacggtgtcc aatacaagca attttttcat gggcaatctg taaaatatcg tgtattaaga
     4861 aaaatcctgg gaaaccattg tgctcgaagt tgggttgatt attttggcac agaagacaaa
     4921 actcaaatag aacaaaattt aactcaaaag caggtagtat gggattggat aaatcatggg
     4981 aatgatttga taatactaaa ttatttgccc ggagcattaa agcatcctct aactgataaa
     5041 atagcctggt ttaattcatc agcttatttg aattattatt ccaatttgaa ttatggtgaa
     5101 ttaaaaaacc tgcgttcatt caaatattgg gcaagccggt atcttatcct gaaagatatg
     5161 cttcccatga tctgtcatta tggtaatggc caggcgtttt ctgcgaaaga aatatctgaa
     5221 attaaccagg ttattcagcg tcatactcgt gtttttcatt ggcaaaaagg agatttcatg
     5281 attgtggata attttacctt catgcatggc aaacaagctc atgttggaga gagattattg
     5341 tattcatgca tgacggctta ttaatgctgg attattttta tccgctccct gacagttgca
     5401 gctcagtgtt tgaaccataa tccgagccgc gactgttagg gagcggatat ggtattccca
     5461 tacaaaatct tgctgaattc tggctatact taaggtaaat agaccttcca ggttaatttg
     5521 tttatactca aattctctct ctgttatggt tgtttttatt ataactcttt gccagacaaa
     5581 taatttttgt gcgtagccag gcactttcag gagcattgta agtggtaaac gtaactatga
     5641 aaaacaaaac caaagacctt ttgaatgaac ccattgccat cattgggatg aattgtcaat
     5701 ttcctgggat tgattctgat atagaagatg ttagtgcctt ttatgacatg ctgatgaaag
     5761 ggcaaactcc aatcaaagat gtgcctgaaa atcgctggaa tattgaagaa tactatagtg
     5821 ctgacagaaa aaaagcagat aaaattatta gcaaaaaagg cggcttttta gataacaccc
     5881 atttattcga tgctgcttat tttaaaatat cttctgccga agcgaagcaa atagatcccc
     5941 aacaacgcct ttttctcgaa gtggctattc gagctttaaa tgatgccaat atcccactag
     6001 actctttaaa agactctaac agcggagttt attgtggcat ttccacacac gaatacagcc
     6061 aactgaacta taaagaccat atcaaattta atgcctacac acccattgga atagcgaata
     6121 gtgctgccgc tggcaggctc tgccattttt tgaatttaaa aggacccagc atggctgttg
     6181 atacagcatg ctcttcttcc ttcactgcac tctatctagc ggccaccgca ttgagaaacc
     6241 agcaatgtga tatggcaatt gtcggaggcg tccatttgag tctctgtcct gaaagtttta
     6301 tcggattaac caaagccaat atgctgtcag cgacaggtca atgcagcagc tttgacagta
     6361 aagcagacgg ctttgttcgt agcgaaggtt gcggagtggt tattgttaaa cgcttaagtg
     6421 acgccatacg agacaataac aaaatctatg cactcatcaa aagcatggtt attaatcaaa
     6481 atggcgatgg aacaggcctt gcagccccca gtactaatgc ccaaattgcc atgcaccaag
     6541 ccgcattgaa acatgcccat ttaactgcag gtgaaattga ttacattgaa acccacggaa
     6601 caggcacaac agtaggtgat cccgtcgaat ttaatgcgat ccaaagcatt caccaggggc
     6661 atcactctgc tcaaaaaccc ttgatagtag gagcactgaa aagtaatata ggccatacta
     6721 tttcatcatc aggcattgct tctttaatta aagtactttg ctctttgcaa aatgaagcga
     6781 ttccccccaa tttacattat tcaaacccga acagcttgat tgatcctgaa agtatccctg
     6841 cgcttctacc cgttggagca attccttttc ccaaactcaa aaataaaaaa agatatatcc
     6901 aggtatctaa tttcagcttt acaggcacga atgtaagtgt cattttggaa gaagcgcctg
     6961 agttggaatc acacgagtca aagcaagaca acaatgaacc aaaatgtttt gttgcctcag
     7021 ccaatagtga actctcattg aaacaattga tgtcccgcta cgaacactat ttgaaaaatt
     7081 cttcctcaac cttgggcgat atatgtcata ccttgatcaa ttgtagagac cattacaaat
     7141 ttcgctgcgc catcattgta aacgataaaa aagaactcat taaaaaaatt gaatcgaaag
     7201 actatgaaat aagaaaggta gttctacaaa acaaccccag aacggtattg aatgatgcca
     7261 aagccattta cgaagcttac ttatccggta ttaacatcag tactgatgat gaaaatactg
     7321 catacaacaa agtggattta ccgctctact gttttgacag aaaaccctat tggcatgaag
     7381 tcagaaaaca agtcaagaat gatcattggc tggattcttt atatcaacaa ccaatagaac
     7441 aacaagttgc aaccattaaa acagagctcg catctcaaat caaagatctt ttaaagaaag
     7501 acaatataga tccgcatcag gatttggaaa ctttaggttt aactacctcc ttattggaat
     7561 ccctggatca aatccttcag gatatgtttt ccccccgata taaggcgcct tctctccaat
     7621 ccttacgcta cttaaccctg gataaactgg caagacattt acaaaaaatg atcatgccgc
     7681 ctgtcgttta ccgacaaccg tcaattaatg tactcaatgt tgaacctatt gcaattatag
     7741 gtatgagttg tcgtttcccc aaagcagcca atgtggatga attcctgtct ttgctggaaa
     7801 aaggcgaaag cggcatgatc gatatcccct tagagcgctg ggataacgaa aaattctatg
     7861 actcggatgt tgatgcatta ggaaaactgt acatcaagca gctgggcttc attgatcatg
     7921 tcaaccattt tgatgcggag tttttcaata tcagcccgcg tgaggctaag ctcatgtcac
     7981 ctcaattgcg tgtttttatg gaaaccagtt atcacgcact tgaagatgcc aatctttctc
     8041 ttgatgcggt caaagacact aatacaggag tttttgtcgg agtggggact aatgaatatc
     8101 cccgagtttt agcttatcaa ggcgttactt tagaagattt gaatatttat tttgcgacgg
     8161 ggaatgtttt aaatgcgctg gccggccgtg tagcctattc ctttgatttt catggtccaa
     8221 tacaagcaat agataccgca tgctcatcct ccatgacggc catacacaat gcctgcctga
     8281 gtctgcaatc cggtgactgt aatatggctc ttgctggcgg tgtgaacgtc atccttagcc
     8341 ctgattctaa cattactctt tcaaaagcca gaatgctctc acctgacagc cgctgcaaaa
     8401 ccttcagtga agacgctgat ggctatggcc gcagcgaagg gtgtggtgtg gttgttttaa
     8461 aacgtttaag tgacgccgtt aaagacaacg ataatattct ggcagtaatc aaaggcacct
     8521 ccatcaatag cgatggtaaa agtggtggtt tcactgtacc taatggtgct gcgcaggaag
     8581 aagtcatcct aagcgcattg gcaaaagcga acctgtcacc tggtgatatt gattttgtcg
     8641 aagcccatgg gactggcacg ccattagctg atcctatcga agtgaatacg ctgaccaaga
     8701 tttttagtga gcatcacaac aaagaaaacc cgctttatat cagttcagta aaaaccaata
     8761 ttgggcattc ggaaagcgca tccggtgtag ccagtgttat caaaaccgtt ttaagcctga
     8821 aaaccaaaac aatatttaag catctgaact tcaaaaagct taatcctgcc attcaattaa
     8881 ttaatacagt aatccctctt gataaaaccg attgggccaa gaaacaaggc ttaaggtgtg
     8941 ccggggttag ttcttttggt tttagcgggg ctaatgccca tgccataata caggaagctc
     9001 ctgaagacaa aaaagaagca cgcgcccttc ccgaagaatc tctgctcgtt ttatcagcaa
     9061 aaagtaaaac ctctctggag ttattactgg ccagttacca aaactattta tccaataccc
     9121 gggaagaatt tgccgatatc tgctacacag cagcaacttg tcgtagtcat tttatgtatc
     9181 gtgtagcaat taaagcgcaa aatgcagccc aagcggccaa tatcataaaa aacaatggat
     9241 acactatttg ccatatcacc aaagaaaaag aatggactca acaaccggac acacttgcag
     9301 aattgcaaac agcctacctg gatggattcc ccatcaactg gtctgaattt tatcgctccc
     9361 ttggccatca ttttgcaaaa ataaaattac ccttatacga atttgaacgt atagaacatt
     9421 ggtttggtga caaaaacaag ttgaaagatg cacctctacc caaggactgg tgttttcaaa
     9481 tccagtggca aaatgaacca tgtgataaaa gcaatcgaaa gctgagagga aatagatggc
     9541 ttttaattgg ccccagacat ctggctgatg gtttcaaagc tcaaggcctg gaaattatct
     9601 acgaagatga agagtattct cgagacaaac tggaaggcat catttttgct gaaagtttgc
     9661 atcgggcacc acctgacata gaagctaata ttgattttca aaagaaaacc ttaaaaaagc
     9721 tgctgaactt aattaaagaa cttgataacc agtcaattga tttgcaattg ctcattctta
     9781 ccagcaatgg aattgctgaa ctggcgaatg acaaacaccc cgtcaatata agcaatagcc
     9841 ctctcattgg tttctgcaag actctcgctt tagagctgcc acaattcaaa accattctca
     9901 tcgatatgga taaaatcgaa gaggacaaat acccgacaca agtgatagaa gaaatcaatt
     9961 ataaccatgg aagccgctat gaacacatag tagcttaccg tggtggggaa aggctggttt
    10021 cccgtttaaa aaagactccg ctggaagata ggcggcgatt attgcatgga gtgaaaggac
    10081 gctatcttat tactggcggc tgcggtggtt tgggattggt aactgcccaa tccttgcttt
    10141 cctctggagc aagagaatta attctcacct caagaaatat cgacaaacca aggatcaagg
    10201 aagccattta taaaattaaa tcccattacc ctggcgccaa catccgggtt gtcagtttag
    10261 atattactga taggggaagt ttaaaaaatt tattatcaga gcttaatgcc gatggtcagt
    10321 taaaaggcat catccatgcc gctggtgctg cgattaaagc gcctttgctt gaacatcagg
    10381 atgaagatgt tgattattta ttttcagcta aagtaaaagg cggatggtat ttacatgaac
    10441 taagtatgga ttgcaacctg gatttctttg ttgtctattc ttcagtttcc tccgtatttg
    10501 gaagcaacaa agaatccgtt tatagtggaa cgaacagttt cctcgacgcc ttgatcgcgg
    10561 agcgccaacg tatggggtta cctggcaccg ctgttcaatg ggggccttgg ggtgaagtag
    10621 gaatggccca aaaacgttcg cgtgatgaag gactaaaaca agcgttaatc agtaatgcac
    10681 aaggtcatgt aatcattaaa tgcctcatca acgacgaatt caaacacgcc actatcatat
    10741 ctccagaata cctcaaattt atgctggatt ttgtacccaa gcccctacct gggttttatg
    10801 gacaacttga tgaagagctt gaaaaaatac aacaacatca tcaaagcccg gcaaaaaatc
    10861 tgtctccatg gcttaatcaa tatctggaaa ttagtgaaga ggagcgattt aaagcctgta
    10921 aagcaatggt atctgatatt tgcacaggaa tccttgaact gacagaaaat gaagacttaa
    10981 atgaagacga aggttttttc gagttgggtt ttgactcgct catgattact gaattggcca
    11041 gtgaactaaa gaaaaagctg gaaccattcc ttaaagttac cgtgaccatt ggttttaatt
    11101 acccatccat cagtaagctg gccaaataca ttgaatcaga acttgataag aacttggtca
    11161 agatacaaag tccaaaatca gctctcaagt cgattgatga tggtatagcg attataggca
    11221 tgagttgttt attacctaat gcgccagaca tcgctacctt tgaaaaactg ttggaagagg
    11281 gattaagcgg tatcaaagac attccaaatg accgctggga taatagccaa tattacgatc
    11341 ccgatatgga agcgcctggt aaatcctacg tcactaaact gggattaatc gaaaatatta
    11401 aatattttga cgctgcattt tttggtatta gtccccgaga agcaaaattg atggaacctc
    11461 agcaaagagt atttcttgag tgttgctaca aagcattgga aaatgccaac tacccttcgg
    11521 aatctttgcg tggcagctta acaggcgtat ttgcgggcgt aggtcccaat gaatattatg
    11581 cgcaactgga aaaatcaggg ttttcaaatg aagaactcag cgcttactcc atcacgggta
    11641 atgtgctcaa tctaatccca ggccgagtag cctacacttt tgattttaaa ggcccatcaa
    11701 tcagtgttga cacggcatgc tcttcttcgt tggtcgcaat ccattacgcc tgccaaagtt
    11761 taaaaaacaa ggaaattgat tatgctcttg cgggtggggt gaatatacta cttatgccgg
    11821 aatctaatgt aactttgtgc aaagcaaaag ctttatcacc tgatggccaa tgcaaaacct
    11881 ttgatgaaaa ggctgatgga tacgtcagag gcgaaggttg tggcgtaata ttgcttaaaa
    11941 gactctctga tgccttgcgg gataacgatc acatcctcgc cgttattaaa ggctcagccg
    12001 taaataacga tggtaaaacg gctggattaa ccgtgcctaa tggtaaaagc caggaagaag
    12061 tcatgaaaaa agcgcttggc cagactgaat tgtcgagaac cgacatcagc tatattgagg
    12121 ctcatggcac aggaactccg ttaggggatc ccattgaagt ggatgcaatc aacaaagtct
    12181 acggtcatca acgcaacaaa gagaatccct tatacctggg taccgttaaa acaaatattg
    12241 gacatcttga aagcgcatca ggcgtagctg gcgttataaa aactgttatc agcctgcaaa
    12301 ggaaaactat ttataagaat ttgaatttta ataaattaaa tcccaacatc aaactggatg
    12361 gtactcaact tgctttagaa aaaaccaatt ggaatacgaa tacaaaactc aattacgcag
    12421 cggtcaatgc gtttggtttt agcggcacga atgcacatgt tatccttgcg gagtttccta
    12481 aagaagaaaa gcccaaaacc tcaaaacctc ctcaaaaaca tttgctggtt atttcagccc
    12541 attccaaaac ggctttagat aatttaactc agcgatatca acagtattta gagacaactg
    12601 aacatcattt tggtgacata tgctttaccg cagcgacttg tcgagaacat tatcattacc
    12661 gtttggcctt aaccgctgag aatgcggttg aagcgagtcg tatgctgggt gcagggcaat
    12721 ttgccttgtc ttatcatgaa aatattacac tggacttaca aaacgacaga gcgcttaatt
    12781 ccctgttaat ggattatctg caaggaaaac cagttgattg gaaatcctac tatcaatccc
    12841 ttaaaggaga gtttacaaaa gttctcctgc ctaattatac ctttgaccgc agcgagtatt
    12901 ggctggataa aaaaaacaaa gatagcatgg ccactcataa aggtgagcaa caccctcttt
    12961 tagggcaaat gcttgctatg ccaggaaatg aatatctgtt tcatcagaaa ctggatttgg
    13021 agaatttggc ttatatcaaa caacacagtg tttttgaaaa agtgattttt ccagcaacag
    13081 cctatattga atcgggtttg gcagcagcaa aatccatatt tcaatccagc gcttttcaaa
    13141 ttgaaaaatt caatatcgaa cgtcctctac atcctaaaca aggtcaggag tttcaattac
    13201 aagtcaaacc taaaatggat ggacaataca aaatcaatct tttcgcgaaa caagagaacg
    13261 actggcaaac tttctgtgaa atggaaattc atgccaagcc gacagcagcc cgggaatcta
    13321 tccatattga cgtgcttaaa tcatcctttg gcgataaagt agagctatcg gacatatatg
    13381 agcaatttca gaaaagctca ttattttacg gcgatgaatt tcgtgtcctg caagagggct
    13441 atgttcgggc gaacagtgtt ctggctaaag tcacattgac caaggcaagc caggaccagg
    13501 actattatta tcaccctgtt ctgcttgatg gtgccatgca aagtatcttg ttattaagtg
    13561 ttaaaaatga cgataataaa aattttaacg ccgagaatgt tacctacata ccctatgcct
    13621 tcatccgcat gactgtattt caggaagcgc cacgaagcct atgggtgcat ttaactaaac
    13681 aaaatactga agatgcgagc gatctttgcg ttaacatgaa tttatatgag caatctggtt
    13741 tattaattgc tcaaattgca ggccttaaat tgcgaagggt aacacgaagc cattttgttt
    13801 cctatgacgc tgctttaaac cacctttatt acacggaatg gagccccgtc cctttaaaaa
    13861 ccatacccca acaaaaaatc cctgacttct tggccattac aaaaaatcct ggcaaagcta
    13921 aagaaatatt ggatgggctt aattatcacc tggttgaaag gatcgatgac tgcacaaata
    13981 tcgagaacag caacgtagta ttcctttatg aacaaggaca gtttcatgat ctttttcatt
    14041 gttgccagat cttatttcga ttgcgtccca agcgctttat actggttacg gaacatgctt
    14101 atgccattca agaccatgat caggttaatc cttaccacac aatggcctgt tctttttgga
    14161 aaagctttag aaatgaatat gagcccaata aaaattatgc cattgattta gactccagga
    14221 gtcgcttggc tactgcgtta atgtatttat tgaatacaga cacccatgac actcaaatag
    14281 cagtcagaga atctctttac ttacccaggc taaagaaaaa gcaattggtc agtaactctg
    14341 ccccggagag attaattgat ggcaatgcca cttatcttat tacaggcggt actgggggat
    14401 tagccaaacc cttgatggaa tacctgatga ggcgcggtgc caggcacatt gccattacca
    14461 gccgcgccca ctatcaggat gacattaagg cattaataga taaggccaaa caaaatcaga
    14521 ttgatatcaa acattacatg gttgatgccg gaaattatca gaaaatggag caagtgattg
    14581 gagaaataca acagagcccc aaccgattga aaggcgtatt tcatttggca ggtttgatac
    14641 aagatgggct catcgtcaat ttgaatgatg aagcaatgca aaaagtaatc agtgcgaaga
    14701 tggatggcgc tttaatttta catcaattaa cccaaaatat cccattagaa tggttcgtca
    14761 tgttttcttc atccgcttcc ttattaggtg ccagaggtca ggcaaactat gccgcagcta
    14821 atgggttttt agatggttta gcccatttaa ggcgccaaca gggcttacca gcaatttcca
    14881 tcaactgggg acctttccat accacaggaa tggcagccaa tttgactcat actctacagc
    14941 attatggttt tttaccatta gataaagaca acattgatat tcttgatgtg ctcttgccta
    15001 gtcaattgcc tcaaatttct ccttgtccta tgaattggga tgtttactgc aaatatacac
    15061 cgaaacaaac ggaattatcc gcgctggtta aggcgaaacc actacctgat cagtcttttt
    15121 tgaatgcact tcgacaacat tccaaagaag aaggcatctc aatattgtca caagcattac
    15181 gagaaattgc ggcggatgtc ctggctttgg atgacagcga acacatttcg aaccaccacg
    15241 atttgttttc aatgggtctg gattcactga tgtcaatgga tatcagaaat cgcatttatg
    15301 acaaattaca atgccaaaca ctaagcttac cgattgaata ttttattaat actcctacca
    15361 ttaataaaat cgccagacat atttctgatg aattagacca tgtttttgat agtcactctg
    15421 actaccaccc taccgaaaat ccgtcagaaa gagaaattgc attgtgtgat tttcaatatg
    15481 tattctgggt gcttaacaaa ttggattacc attttaatat cggtacacaa atacaattac
    15541 acggccgaat caataaagat tatgtccaac gggcttttga atttgttgtg aatcaaaata
    15601 gtgtcttctg gatccatttt aataaggata aaccaaccca aaaattgcat aaagttggac
    15661 aatttgaatt aatttaccag gatatttctc tgaatcatga aactaaggcg ctgaatcacg
    15721 aattccaaaa caatatattg cgcgctattc cactcaccaa acaacccttg attagagtgt
    15781 atttgtataa gattaataat gacttgcatg aattacacat cgtcataccc catattattg
    15841 tcgatgacgc atcatgcgaa atggtttttt gccaatttaa aaacaattat gaaaaactaa
    15901 ctcttgggaa agaattagta tctaccccag aaaaaggctc ttattttaat tatgtgaaac
    15961 aaaataataa tcactatcaa aaaaacctga aaaataaaat tgatttttgg caaaactata
    16021 ataaaggctt taaaatgctg agtttcggca atagatatca tttatccgat acctcaaaac
    16081 aaacaaagca cttatttcat taccctattg atcctcaaca cgccgaacaa tttataaatt
    16141 ggcataaagc ccaaaatatc aatgtcagta ctggattgat tgccgcatgc caaattgttt
    16201 tctacaaact aagccgccaa aataaaatac cgatcatttt aattcacagt ggtagagaag
    16261 gtggggaata taaatcaacc ctgggtttgt tctcggaata taaaaggata aatcttacct
    16321 taaatgagaa tagccaattt attgattgta ttcattcaat tgaagagcaa ctcttaaaaa
    16381 cagctcccta tcaaaaatgt tctcatctca tcaaagacag tggcttgaaa ggttcgagct
    16441 tatcctttgg tcaatatttg actttggcgt taaataagat gtttatgctg aaacacttta
    16501 aagaaagtaa gctccattcc attatcattg actattacct gaaatattta agtcgcctgc
    16561 tatccttcaa aaaaaacatt tcaatgaaac aacgactaaa tcaactattt aaattggata
    16621 tgtcattgca aaaaccggaa agattaagag ttctcgttag cgtaacacca agctttttta
    16681 ccaaagaacg ccctgatatg agttttgcta atcttgatta cagctatcct aatcattttg
    16741 gatgtatgga taggccgata ggaaatcgaa cgctttggat ttatttctcc aaaaatcagg
    16801 atggtgaata tcaaatttcc attaatgcgc cattaacaat agaatgtaaa aataaaatcg
    16861 gaagcggtct tagccagttt attacaactt ttgtagagaa taataattgt aatattgctg
    16921 aattaattca agcaatggag gattcctcca ttgcttcctg attttactga gcctggttaa
    16981 ccgtagtatt attatttttt atttgtttag ggtttgatga tgaaggctga gttgaaactt
    17041 tgggtaccac aatatccgtt ccaaatttgc cttttatctt aacctcacct ggctgcaata
    17101 cagcagattg gccattggct ctacgacaac gctgggtatt acatttcatc ttacaaaccc
    17161 cctggacaca tccatagcat ccccagttat cccaacagca agcccaattg ccttcgattg
    17221 ttgaggtaaa agttccccct gccaactggc agacatcttt catttcagca tttgatgccc
    17281 ccgtattcgg cgcagcataa acatgaattg atacaaggct aaagagaaag aggaaaaaca
    17341 cccctgccat ccttaaagat ttcatgattc acctccttgg aatcgtacta tcaggacttg
    17401 ctaaaaaacc caatttcctt gttaaaaatt gctttcaatt tggtcattta gtttatctaa
    17461 actccctcat taaaaacatt tctttgcctt gaccttgggg cttttcgtaa gccctaacta
    17521 tttaattatg gcaaaacccc ataattgttc aagttttaaa caaaaagagg tcttgaaaca
    17581 ctgaaaagca tcccctcagg ttacttcatt ttttctatgc caaatactga atagaataca
    17641 aaaaaccaaa gttgccataa caatacctag agcaacgaaa atcggtatct tgaaccaagg
    17701 tgcgataagc atcttaaagc ccacaaatac taaaataaaa gcaagcccat atttgagaaa
    17761 ataaaatcgt ttgtgcatat tcacaagcag aaaatataaa gcgcgtaatc ctaaaattgc
    17821 aaatatatta gaagtaaata cgataaaagg atcattggtt atggcaaaaa tcgctggaat
    17881 gctatccaca gcgaaaatca gatcactgat ttcaataaga accaacacca taaaaagagg
    17941 cgttacgtag acaagttgat ttttcctgag aaaaaatcgc tcaccatgta actcctgggt
    18001 aattcgcaaa tggcttcgca tccaatgtag tatggggttt tttgcgagct ctggtttctt
    18061 atcagcaaaa atgaacatct tgattcccgt tatcatcagg aaaaatccaa aaatataaag
    18121 aatccaatga aattgattca ctatccatat cccgaataaa ataagaatca ggcgcataac
    18181 gatggcccct atcacaccat aaattagaac acgatgttga tatttagccg gtattgcaaa
    18241 atactcaaag agaataagaa aaacaaaaat attatcaaac gataaagact tctctatcag
    18301 ataacccgta aaaaattcaa gggctttttc attggctact gaaaaagtaa aattctcaac
    18361 aagataccac catagaagaa aattgaaaat aatagccaga gtgaaccaca atatggtcca
    18421 ggataaagct tcttttgtgg aaacacgatg agcttttctt cccccaaaca caaacaaatc
    18481 aacaaacagc ataatgagca caaatgctaa aaaacctaac cacatccacc attcactgat
    18541 tgaagtcatt aatttctacc atgtcttttc tatggttttt atatcacatc gatatgtttt
    18601 cattatagtc atagtattag aaattatgcg cttcaaaaaa tatttatatt catttgaaag
    18661 aaacctagga agggggagga gaagaaggag ccaaagtagc gatcaattga ataaccccaa
    18721 actcaccatt ggttttcctg cctacagtta attgaatatt cagtaattga gaaactcgca
    18781 tggtatcgtt tttataagtg acttttaagg gcaatgctat aatccaatgg ttctctctgg
    18841 tttcaatgaa ctctgctacc ccattaactt cactagtcat agtgagtctt tgcattttaa
    18901 ttgcctcaat attccctgat ttcatcatgg cagaattaaa ctcaatccat ccgttttctg
    18961 tataacatga ttgtaattta tttagctgcg actcaataga agtaaaatca aaagcaaaag
    19021 attgtgttgc ggcgtgttca gcccattgta aaataatatt tttatctgtt tttataattt
    19081 cttcagggat tttatagcga caagaaaccg ttccttttgg ctttgattgc tcaggttgtt
    19141 taggttgaat gttttgagag ccgagaaaca attgaggtga tagctgaaga gaagatgagg
    19201 ccagtatcga ttttaaagat tgtagcacct tattagaatc aacgctctta gtctttacca
    19261 tcaaattatt tatcatcttt tgatctaaag ctaaattaac atcaaggcgt tgagttattt
    19321 tctcatcgtc tttttgataa acaactttta taggtaattt aatatcccat tgattttctt
    19381 taacttctat aaactggggc tctccttcta tctgagcact taaaattggc ttttgcttat
    19441 tgattgcctc aatattatct gatttatctc ttatggattg ttgatattga gaaaaagcat
    19501 gccataaggg taagggatta attcgagtaa tcaatgaatt aagttgtaat ttagaagaat
    19561 gcaaaccgat ctggcttaac caatttgtat agagttttcg acctttgttg ccaaaatcac
    19621 tcagagtgct tttgttagaa tcagaatcgt taacaaggga tctctgttgg gatacagcaa
    19681 gatatggagt ttttgcggat tcaggcacat caaatgattg ccttcccaca agattataag
    19741 tgggatttaa gtttggcatt ggttgaacaa ttgccactct ttgcgcagcc ctgttactca
    19801 aagcaagatt tcttttagaa cgttctccat gatttgaaaa taaaggcaca aaaaaagaat
    19861 ctgttatttt tcttgcacta tctaaactgg ccttttttct ctcagccagt attatgccca
    19921 tacttttaat tacctcatca gctggcgtca ccttctgagc tagtggcgcc agacggggaa
    19981 tagcaatcat ttgcataatc cccaaactat ctttattttt ccagcctatg gtcagatagg
    20041 cattaagaaa atgtgtcact ctttttttat cattctcata aacaactttt aagggcaata
    20101 taattttcca tcgattatcc aaagcctcta ttattttagc ctccccatct ttatagccct
    20161 ttacttttaa tttttctgct ttaatgattt ctatattatt tgatttatgt aaagcatttt
    20221 gcatttgccc ccaccccttt tcagtatagc aagagtgcaa ttgatttaat tggttgtcta
    20281 tagacgcaaa attgaaatta aaagatagaa tagccgcata ttcagcccaa tttaaaatga
    20341 tatccgaatc aatatgagaa atttcaggtg agattttata attacaatca ataagattgt
    20401 ggtaggcaat accctttttt tcaatggatt cggttcgcat tgcagcatct ctgctaacag
    20461 catcctgagg gtaggctttc atactcaata aaataagaaa tacaccatag aatagatgtt
    20521 ttgacataaa aagattcctt attctgtatc tacaaatccc ttacgaacgt gcaaatttat
    20581 cggataattt aatttattct aacgttagtt acggataaaa tcggtcacta tcttgtatcg
    20641 ttcgggctga ggaggcattt atgccgtctc gaagccttgt atggaattta atgtttcttg
    20701 ccaaggcttc gagacgatgc ttacgcatct cctcagcaca aacgggcctt ggatatcgac
    20761 tgcttcttat ataattctta tccataactc acgttattct actgtttaat agtgtggtgg
    20821 taactcatta atttttcaaa tactttagta ttctaatttc gatccttgga aagaattgct
    20881 attcagcatg tgtgttcttt ttagactata aaaatctatt attttgctac ttgaaattgt
    20941 atcaatcgac cctatttaaa cttacaacta aatagggttt tacacctcaa ttgtgagacg
    21001 tggagcagcc agtaaggtgc tcgggtaata ttaatcttgg cctcaaagtt aaggagatat
    21061 attatgaaca ctacctcatt atcactaacc ccgttactac ggcattccgt tggttttgaa
    21121 cgattcaatg atttgtttga atcgatgcga aatgccgatg attcaaccta tgcctatccg
    21181 gcctatgaca ttgaaaaaca tggagaagac agttacacga tcacaatggc tgttccagga
    21241 tttcaggaga gtgatctgaa catcatggtg caaaatgacc agttaagagt tagcggtcgc
    21301 attcaggaaa aagaaaccaa ggagtctgaa tacttacacc gaggcattgt gacgcgagcc
    21361 tttgaacaga cctttcgact tgcagatcac atgaaagtaa caggtgccga aataaaaaat
    21421 gggctattgt ctatcagctt gattcgtgaa ataccggaag aagcaaaacc acgcatcatt
    21481 ccaattaaga gtatttctga tcaagaaagc aataaaaaga gtaaaaccat tgaaccagaa
    21541 agcaagaaaa aaatttccaa ttaaaaaatg tgccgggtga tatggaaaat ccggcacaga
    21601 tttgagataa aatagccaga atactcctgc acatgttctc tttaataatt ttttacaata
    21661 aaaataccag ataaactata ctttcaattt agaaagcaca ttagtattac agaggctcaa
    21721 gcaaacaact gaatatgagg ccattatgaa gcgtggttta tttgtccatc tcaaacatga
    21781 cttaccagca tccattgttg ttttttttgt ggctttacct ttatgtctgg gcatagcatt
    21841 ggcttctcat gttcctcttt tttcaggaat tattgctggt gttattggtg gagtagtcgt
    21901 agggtttatc agcggctcag cgcttggagt gagcgggcca gcagcaggac ttgtagcaat
    21961 agtcattacc tctgtagagg cattaggcag ctgggaagct tttttgttag ctggtgtgat
    22021 tgctggaata ttgcaattaa ttgcaggttt tctgcgaggt ggaattattg cttactattt
    22081 tccctcctcc gtaatcaaag gcatgctcac gggaatcggt atcattattg ttttaaaaca
    22141 aattcctcac ttgttagggc acgacgtcac gccacttcta ttgagtatgt cggatggtga
    22201 attggatatt aatgacatcc gagaagcatt ggatgatatt aatcctgagg ttgttattat
    22261 cagcattgtt tctcttttaa ccctgatttt ttgggagaaa gttctggtaa aaaaacacaa
    22321 actgtttgaa atgatacaag gcccccttgt cgtggtcatc ctgggaattg ctttgaactt
    22381 gttttatgaa caccgactaa caagtttttc ccttgatcca tcggaactgg ttcaactgcc
    22441 gaaatttaac aaagcatcag aattatttaa ttatttaaca ctgcctgatt tttcgctaat
    22501 tactgatttg aaaatataca aaatagcaat cctcattgcc ctaatcgcca gtttggaaac
    22561 actgctctgc gtagaagctg ttgataaact ggatccctac aagagagtaa cgtctacaga
    22621 ccgtgaatta aaagcccagg gggtaggaaa tatactctct tgcttgattg gcggactacc
    22681 gattacacag gttattgtgc gtagtagtgc caatgtggct tttggagcaa aatccaagct
    22741 gtccaccatt ttacatggct tattactttt tatttgtgtc atcagcatac cagagatact
    22801 caatgaaatt ccacttgcat cattagccag tattttgact gttataggct ataagttagc
    22861 caagccggct ttattcaaac aaatgtataa aatgggatgg gaacagtttg tgccatttct
    22921 tattacaatt gttggaatta tttttagtga cttgcttttc gggattactt tagggttttc
    22981 agtagctatt tttattatct tgcgccatca ctttcttaat tcacatgata ttattaaaat
    23041 ccgtggcaaa aacaaaaccc aatactgtct gaaactggcg gaagaggtaa cttttttaaa
    23101 taaaggaagt ataatcaacg aattaaagaa tataccagaa ggtgcagagg taattattga
    23161 tgggtctcga tccaaaatta ttgatcatga tattaaagaa gtctttcaaa atttcatttt
    23221 gaattcaaaa actaaaaata ttcaagttaa attaatagga atttaaatca tgtggacttt
    23281 aaccaaagaa caacaacaag cgattactcc agagaaagct attgagctct taaaagaagg
    23341 caataagcgt ttcgttagta accttaagtt aaatcgcaac ttgctccagc aagtcaatga
    23401 aacatctcaa gggcaatttc catttgctgt tattttaagt tgtatggatt cacgcactcc
    23461 agcagagctg atttttgacc aaggccttgg tgatattttc agtattcgcg ttgcaggtaa
    23521 tattttaaat gatgatatcc tgggcagcat tgaatttgca tgtcaggtag taggtgtcaa
    23581 gctgattgca gttgtcggcc atactcagtg tggggcaatt aaaggagcct gtgatggtgt
    23641 aaaattgggc aacctgacga acctactgaa taaaatcaac ccagtgattc aggaagccaa
    23701 aaagctggat gcaaagcacg atgttcatag cccagaattt ttgaattgcg ttacgagtct
    23761 gaatgtaaaa cataccatga atgaaattac ccaacgcagt gagatcgtac atcaattagt
    23821 taatgaaaaa agaattgcga ttgtaggtgg tttatatcag cttgagacag gagaggttca
    23881 attttttgat gagtgatatc atgatattaa taactttcaa taattagtct tttcaactgt
    23941 gcaacatttc tgatacctaa agcttttacc aggaattgat gcgggcttgg tgcttatagt
    24001 ccgcattcat gaatcaatga tattttttat tcaggtaata aaaccaaaat aaaattccga
    24061 aatagagaaa aaggggttta ataataaaat taacaactaa tataaagcgt aaaaaaaatt
    24121 cattggacaa ccacaagacg cggcacaaag acaaaagtga aagcgttgaa taatcagaag
    24181 gaatatacaa tagtactgtt actatcttaa tcaaataagt accaactaac ataaccagta
    24241 aagctatcag atgacgtaag gaatataaaa ccgaaataag cattttcttt tttacagaat
    24301 gattcattta cactcactct aatcggtttt acccttacta acttatagaa aataactcac
    24361 ccagattttt cttcaatgca ttttgataaa caacgttggc aacacttaaa tcctgtattg
    24421 ccaaaccaac ggatttaaat accgtgagct tatctttgca gtcagtattc ttttgtaata
    24481 accattggcc gatttcaata atggattcct tttttaaatg attgtgctct actgcactaa
    24541 tgatttcgcc tgactcagct aaaacagcgt ctaactgatc agcaatcacc actgctcggc
    24601 ttaacacatc atggctaatt tctttcattg aagggtgatg ggagcctatg gcattaatat
    24661 ggacatgtgg cgaaatgtct ccaaaatgaa tcattggttc tgtgctattg gttgctgtac
    24721 aaataatatc ggcatctttc actgccatgg cgacagaaga acaaattgtc atatcaaatt
    24781 gatcttcgaa ttcgttagca aatttttcgg catttttaat attgcgcgac caaacaaaaa
    24841 cctgttgtat atctcgcaca gaagcgactg cctcaagttg tgttttcgct tgagcacctg
    24901 acccaatgat cgcgacctga gtcgcattat cgcgtgcaaa atattttgtc gccagccctg
    24961 aaacagcgcc cgttcttaag gaagtgaggt aagcagcatc cattaatact ttagcttcac
    25021 ctgttgttgc gtcaagcaac ataataaaac cagtgatagt aggtttattt ttagctaaat
    25081 tttcaggaaa aatggatact acttttaagc ctaggctttt ttcctgggca agataacccg
    25141 gcatagtcaa cattaatccg ttttcttctt caatggtaac acccgttctc aagggcaact
    25201 tcaccgtgtt atttgccaat tgaataaatg cgctttccaa agcactgatt gcctgatgca
    25261 tggtgatgct atgctttaca tcgcttaaag acaatatcct gagactcata tcaataaacc
    25321 ctatacttta gttaactact tagtgactga atagaatcac atcttatggt tttaaatcaa
    25381 ataaagtatt tcctgttttc atatcaaagg gaacagaata atgttcatgc atgattttcc
    25441 attttccatt cattttgcag tgtaccattg tacctcgcat ccatcctcct tgcatttcac
    25501 ctttttcgtt cgttccacca caataagtca aataatagga aaccgccaga ttattatcaa
    25561 ccaaagtttc caactcatgt acttcaaata ctgtaggtcc tggacagaat gaaagacagg
    25621 tttgccaatg tttcctataa tcattagctc ccttaaactg aagctgtgaa actgcatcaa
    25681 aagaacgaat atctggtgca tagtgagaca tgattttatc gatgtcttta tcacgtgctg
    25741 cattggcata ctcttcaatg agcttatgaa ttattgccac ctcattttcc ttattcgcaa
    25801 tagatttcac ctttatctcc tttaattaac ttcttcctga attttaattg tagcaaattc
    25861 tgcaaaaaac ctaatgaatt gttcacctgc aatagatgtt gctaaagcaa catttttttg
    25921 ctttgttaaa aaagaaccta aaatagaatt accctgttcc ctgaggtttt ttgataccga
    25981 tgcagcgaat ggattgcctc atcatataga gaaatctgac tctcaacaga aaaaatattc
//
LOCUS       DASLOE010000027        25997 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.027, whole genome shotgun sequence.
ACCESSION   DASLOE010000027 DASLOE010000000
VERSION     DASLOE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 25997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25997
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.027"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            264..392
                     /locus_tag="U6309_000898"
     CDS             264..392
                     /locus_tag="U6309_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN5504351.1"
                     /translation="MRHIYFALLLIVVTQTSLCLSACGQKGPLYLPEQEKKAPTDQ"
     gene            402..1235
                     /gene="dapF"
                     /locus_tag="U6309_000899"
     CDS             402..1235
                     /gene="dapF"
                     /locus_tag="U6309_000899"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEN5504352.1"
                     /translation="MGIKFTKMHGLGNDFIVLDGVNQSIQLTVEQIQKLANRHTGIGF
                     DQCLLIESSQTEGIDFNYRIFNADGQEVGQCGNGARCIALFARYYGLTAKNKLTVATK
                     TTLMDLIINEDNSVSVNMGVPRLAPSEIPLLADRQSPEYSLELNNGNTVNLHAISVGN
                     PHAVLLVENIDTAPVNSLGQQISFHPQFPEQVNVGFMQIVNHEKINLRVYERGCGETI
                     ACGSGAVAAAAIARLFYNLSDKITVHLPGGDLCIQWPCPTTPIILTGPAAFVYEGTLL
                     S"
     gene            1546..4236
                     /locus_tag="U6309_000900"
     CDS             1546..4236
                     /locus_tag="U6309_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0365 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504353.1"
                     /translation="MVDFDKPVSKNDILASLLLIADLNRPSLNQPLSWEQALKSFLSL
                     NDPLIKTEIFIKWLSQLNPDQLQSSPEIVLLLKHIVEQKLLPYTLKKILIQRENFTTS
                     QINWLCNHCLIVAPDQTTLPDIIKLGSWSWLENHMKRNELLNFDLLKIAILNKDYLAS
                     INNSKESQLDFIYALNANKFNYQQILELIALAQDSQIKSLLITCLLSKKEYLDNLKEK
                     SFLAHLNQSEFHSPPRLNALIHQLNLNCLTVAQIELLQPEACVTLLCSIPHFHQLSEK
                     QVEKILLKYPKPDMIAYWLNHYSSRPNAHFILAHLMNLVDVHVVDELNKMDSTKKEAI
                     IMNMVEHLELFDPTHPLLINQNEEKRLITAIQRYLYGQHHDAYVTYIKLLVYKLLNRN
                     YQFSPQAMQLLICLNNLEEFKELTNKIAYLTNYYLRALAQSGDSSLFYNDSRINITSM
                     TQLVQLAPKIPTKSKEKSLLSSLFCGIKINESSVMTPTNDIPEHPLINKLIKSKRTVK
                     SIDYFLMHYKGDKQVLSKIINDYLSYYVQEGCTEIHRRSIYHLIVLMSLSDISASVRE
                     AIYTAFLNYPELHDEQINFSLFIYDAKRTIRHFGAQASEDSYARVIDLCEGALKKLNP
                     NKHKEIISIATQALTEAKIELSFGENTGFIAQLISRLKRCWYYGWTGFFSPNPPTFVA
                     AIDSPIKQSEKKTSPLLQQRKYFTTKLETNLSIILDDIKSGYTQQKLNELIEALSIFS
                     LQSNPKNELKIRQKLHRLFHYLLLESKNDLTLDAWLIKNQQLLIANRFRLLEMQLMKG
                     ARNEVESLLKEFDEDSTHFSPISKEFTHSLPEQNPYKCESVSSNQTPGATILDSATEL
                     ATSLKSTLGNLGVFFSNNIMSAIYPNNKSTPQSTSTMISP"
     gene            4422..4721
                     /locus_tag="U6309_000901"
     CDS             4422..4721
                     /locus_tag="U6309_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0364 family Dot/Icm type IV secretion system
                     effector"
                     /protein_id="HEN5504354.1"
                     /translation="MDERRKYFRLKNHGEINASLDNNPIEIVEISSNGAVVVKQKTDI
                     PKGGMLKLQIHNFIMELCYEVIRAEDSNIVLHFTKEDETNKLFLVLKRLRDERKK"
     gene            complement(4837..5682)
                     /locus_tag="U6309_000902"
     CDS             complement(4837..5682)
                     /locus_tag="U6309_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEN5504355.1"
                     /translation="MNNLAQQIEALPITLAGRFIYRFLPYRRRLIFSNISQVYNDQLN
                     ESQKKRLVKAYYSHLAKSLKEALQLRFMSEKKLRAQVEVIGHEKMLAVVAQKKGVLVV
                     TGHFGNWEFAPLGGVLNFKEFKGQFHFIRRTLRFKFIERIMFKNYYQAGLNVIPKKNS
                     LEQVCVALEQNHAVIFVLDQHASLVNRDGIAVEFFGKKAGTYRSLATISRHTGIPVIP
                     AASYRLPNGKHVLEFHDPIPWKDYDTTQESLYRNTLAYNQALEKIILAHPEQWNWMHK
                     RWKLS"
     gene            complement(5675..6952)
                     /locus_tag="U6309_000903"
     CDS             complement(5675..6952)
                     /locus_tag="U6309_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEN5504356.1"
                     /translation="MSQANRVFITGRSALTASGATADETWNAIISGQCGIDEINFWEL
                     SEWSHRLGGEIKDFQPSKMLPDRKLIKVISRQDIMGINAAVQAVEHSKMIAYRDSLAD
                     ADLFNEQTAIYVGSPGNKYFQQYDFIPLLAKTHGDMRSFANQLFDEVHPMWLLRILPN
                     NVLAYTGITYGFKGPNHNVTNHAVGGTQAILEAYHAIRSGQAERAVVVAYDIGTEPQG
                     LFYYEKLGVVSNRHLKPFDKEHDGTILAEGAAALVLESEASVKSRAATCYGEIVGGLS
                     ASEATGLFSIEADGQHLASLMGQTLSTSGINSEEIGLIVTHGNGNSKSDDSEALAIKS
                     VFKQEEVPATAFKWSMGHTLCASGVLDAVLTTYALETKCVPGIANFQQIAPTCEGLLV
                     SREHREIISNSYAMMINRGFASMNVCLVIKACE"
     gene            complement(6953..8245)
                     /locus_tag="U6309_000904"
     CDS             complement(6953..8245)
                     /locus_tag="U6309_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEN5504357.1"
                     /translation="MKRRVAITGLGVVSPLGNDVSTTWKNLIAGHSGIDSIKQFDASA
                     FPTTIAAEVKNFSPDTSISGKHTRFIMSFTHFALEAARQAFEDASIFPTKQTSDRWGI
                     VTGSGMMTAEFEYLTRFQQTCAAGGEIDWGKLQSNSRDFYNLVDFGKTTSNSGLSLLI
                     QQYGITGYASSVHTACASGGQALGLAMQVIRRGEADFMLAGGFDSMINPLGLSSFCLL
                     GALSTYNETPQTASRPFDATRNGFVLGEGAAFLILEEWSKAKARGAKIYAELAGEGNS
                     LSSYRITDSHPNGDGAIQAIKRALEDAGVQPRDVDYINAHGTSTKMNDLSETNAIKVV
                     FGDRIANLPVSSTKSQTGHLIAAAGALEAVLSVKSIEQAQVPKTANLKTPDPECDLDY
                     VVDGPREKSLGVVLSNSFGFGGSNSCLLFKHPEFEEAK"
     gene            complement(8250..9146)
                     /locus_tag="U6309_000905"
     CDS             complement(8250..9146)
                     /locus_tag="U6309_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HEN5504358.1"
                     /translation="MRFLFVDRIVQLSPGEWIKGIKHVTRDDAYLTTDDHGRLCFMPS
                     LIGETLGQLAAWNVMQHNHFTSRPVAGVVSSARLYRPAYVGETILLESNIDSLDEKAV
                     QYHSIAKVGNDIIFTIDGALGPMLPMTEFISTEVIRQQFAEIYRPGEWSSVLALSSPV
                     LIEENCSDCGMINSSMQFDRILASEPGISLIAEKRITRSASYFPDHFPNRPVLPMTVL
                     LECKLNLAKEFIARAKYDRAYRISELRKIKMNEFVHPGDVVVCQVKVKQQMDDELILS
                     YRCEVDGKRVCVVDIVLIPKGK"
     gene            complement(9146..9559)
                     /locus_tag="U6309_000906"
     CDS             complement(9146..9559)
                     /locus_tag="U6309_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN5504359.1"
                     /translation="MNVAEVYPKVREIIADVLVIDEEEISLNSSLIEDLGAESIDFLD
                     LVFQLEKEFKIKIPRGQLEKNARGDLAEDEFEKGGILTTSGLQALKNYLSEVPADRFK
                     ENMKVNEIPVLFTVETFCKLVISAIAQQQATETVA"
     gene            complement(9630..10388)
                     /gene="fabL"
                     /locus_tag="U6309_000907"
     CDS             complement(9630..10388)
                     /gene="fabL"
                     /locus_tag="U6309_000907"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HEN5504360.1"
                     /translation="MSSLVFSGKVGLITGGARGIGKATALKLAHSGCDIAIVYYNSSD
                     EAQSLVNEITGMGRKAVALQADVADCQSVKEMFSQFREHFKHLNFLVSNAASGVLKPA
                     LKMSTKHWRWCLETNALALNHLVTEGLDLMPKNSRVIALSSLGASRAIPNYAFIGASK
                     AALEALVRSLSLELASYGITVNTVSAGVVDTDALKHFPNREQLLDEYQAHALADRALT
                     PEDVANAIYLLCLPEAAMINAHTLYVDAGYSQVG"
     gene            10550..11917
                     /locus_tag="U6309_000908"
     CDS             10550..11917
                     /locus_tag="U6309_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5504361.1"
                     /translation="MKYSWNKTVFPIAAIFSFRLLGLFLLIPVFTLYATNLKGATPTL
                     IGLALGSYGLAQGLLQIPFGMLSDKLGRKPMIAIGLLLFAGGSLLGAVTHSIAGMITA
                     RILQGAGAIGSVLIALLADLTLDEQRTKAMAIVGITIGTSFSLAMIISPALTHYYGLS
                     GVFYLTAALAVLGIIILYLIIPNPSKEQFHADSEPSLSLIKPVVTDQHLQRLNFGIFC
                     QHFILTATFFAIPMLLKYQIENGHLHQQWHFYLPLMLFSFILMVPFIIISERKKRLKT
                     VFVISVLLTTFTQGLLVLTSQNWYWFCILMFVYFVSFNVLEATLPSLVSKLASPGSKG
                     TAMGIYSTSQFLGIFFGGSLAGILYQWYDSQAIFLINMILSCLWLIISLSMKSNVYFS
                     TLILPFTANNEESKLVVNQLLKVTGIKEVILSKDENTLYIRVDNEEYSTGSAEKILHQ
                     STIYQ"
     gene            12001..12480
                     /gene="ssb"
                     /locus_tag="U6309_000909"
     CDS             12001..12480
                     /gene="ssb"
                     /locus_tag="U6309_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN5504362.1"
                     /translation="MARGINKVILVGNVGADPDVRYLPNGNAVTTLSVATSETWKDKT
                     TGEKQDRTEWHRVVCFNRLGEIAGEYIRKGSKLYVEGSLRTRKWQDQQGQDRYTTEIV
                     ASDIQMLDSKGSSATNYDDMPSFQGSSTPQQASTKNQATPTSTAQDAFDELDDDIPF"
     gene            complement(12547..12930)
                     /gene="rplQ"
                     /locus_tag="U6309_000910"
     CDS             complement(12547..12930)
                     /gene="rplQ"
                     /locus_tag="U6309_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEN5504363.1"
                     /translation="MRHRNSGRSFSRTSSHRKAMFSNMCCSLIEHELIRTTLPKAKDL
                     RRYIEPLITVSKSDSVASRRRAFNILRSKSAVGKLFTDLGPRFAKRPGGYIRIIKCGY
                     RDGDNAPMAIVELMDRPVSSDDTEE"
     gene            complement(12949..13941)
                     /gene="rpoA"
                     /locus_tag="U6309_000911"
     CDS             complement(12949..13941)
                     /gene="rpoA"
                     /locus_tag="U6309_000911"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEN5504364.1"
                     /translation="MYTEINEMLTPKVLKVQAESPYKARIVLEPLERGFGHTLGNALR
                     RILLSSMPGSAITEASIDGVLHEYSTIEGVQEDVVDLLLNLKSVAIKLTVGNEAQVTL
                     NKEGPCQVTAGDIQLTHGQEIINPELVIANLNEKGKLNMTLKVERGIGFHNTDAFVRY
                     HDDEIEKKTVGKLKIDNSFSPVKKVAYFVDSARVENRTDLDKLTIELETNGTIDAEEA
                     IRISASILQRQLHAFVDMKFEESRADNKERNDFDPVLLRSVDDLELTVRSANCLKAEN
                     IHYIGDLVQRTESELLKTPNLGKKSLTEIKDVLASRSLSLGMKLENWPPASLGE"
     gene            complement(13960..14580)
                     /gene="rpsD"
                     /locus_tag="U6309_000912"
     CDS             complement(13960..14580)
                     /gene="rpsD"
                     /locus_tag="U6309_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEN5504365.1"
                     /translation="MARYLGPKCKLSRREGCDLLLKSGVRDHKSKCKSEKLPGQHGDK
                     KPRLNSYGIQLREKQKIRRLYGILEKQFRNYYKKAARQKGSTGENLMALLERRLDNVV
                     YRMGFASTRAEARQLVAHKAILVNDKVVNVPSFLVNPGDTVSVRQKAKNQGRIQAALA
                     LSEQRAPCDWITVDTGSFKGTFSTAPTLMDLSSDYNVNLVVELYSK"
     gene            complement(14597..14995)
                     /gene="rpsK"
                     /locus_tag="U6309_000913"
     CDS             complement(14597..14995)
                     /gene="rpsK"
                     /locus_tag="U6309_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEN5504366.1"
                     /translation="MAITKSKQQKTRKKVKRVVSDGIVHIHASFNNTIVTFTDRQGNA
                     LCWATSGGSGFRGSRKSTPYAAQVATERAAAVAKEYGMKSVAVFVHGPGPGRESTIRE
                     LITQDFKIVEITDVTGIPHNGCKPPKKRRV"
     gene            complement(15019..15375)
                     /gene="rpsM"
                     /locus_tag="U6309_000914"
     CDS             complement(15019..15375)
                     /gene="rpsM"
                     /locus_tag="U6309_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEN5504367.1"
                     /translation="MARIAGVNIPDHKHVVIALTAIYGIGKTTSLKLCKTVDIDPSVK
                     VSQLSDAQLESLRTEIAKITVEGDLRRVVTMNIKRLMDLGCYRGLRHRRGLPLRGQRT
                     KTNARTRKGRRKGTSS"
     gene            complement(15453..15566)
                     /gene="rpmJ"
                     /locus_tag="U6309_000915"
     CDS             complement(15453..15566)
                     /gene="rpmJ"
                     /locus_tag="U6309_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEN5504368.1"
                     /translation="MKVRASVKRICRNCKIIKRSGTIRVICKDARHKQKQG"
     gene            complement(15583..16911)
                     /gene="secY"
                     /locus_tag="U6309_000916"
     CDS             complement(15583..16911)
                     /gene="secY"
                     /locus_tag="U6309_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEN5504369.1"
                     /translation="MKNQKHNGSKPRGGLAELKSRLLFVVLGILVYRLGAHIPVPGLD
                     PAKLANFFSEQQNTIFGLFNMFSGGALSRVTVFAIGIMPYISASIIIQLFSVVSPKLE
                     QLKKEGESGRRKINQYTRYLTLLLSIFQSMGMARWLASQQIAMQPDFSFYFTAVVTLV
                     TGTMFLMWLGEQITEKGVGNGISLIIFSGIVSSMPNAIGSVLQQVKEGQMQALTLVII
                     AVVVIVVTGFVVFMERAQRRIRVNYAQRTQGRKVYAAQTSHLPLKINMSGVIPPIFAS
                     SIILLPATLAQFFSQTKGLGWLSDVGMALSPGQPLYLIVYAAAILFFAFFYAALVFNP
                     KDTADNLKKSGAYIPGIRPGEQTTKYIDSVMTRLTLVGAIYLVLVCLLPQILMYTWHV
                     PFYFGGTSLLIIVVVIMDFVAQVQAHLMTQQYDSLLKKANFRGTKLPGLL"
     gene            complement(16914..17348)
                     /gene="rplO"
                     /locus_tag="U6309_000917"
     CDS             complement(16914..17348)
                     /gene="rplO"
                     /locus_tag="U6309_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEN5504370.1"
                     /translation="MNLNTLSPDPGSRPSRRRVGRGIGSGLGKTCGKGHKGQKSRAGG
                     YHKINFEGGQMPIQRRLPKMGFKSRVGRTIDEVSLGELAKLNDEVIDLVALRKAGLIN
                     NSIKDVKVILSGELTAAIKLKGLRVTKGARSAIESLGGSIEE"
     gene            complement(17348..17533)
                     /gene="rpmD"
                     /locus_tag="U6309_000918"
     CDS             complement(17348..17533)
                     /gene="rpmD"
                     /locus_tag="U6309_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEN5504371.1"
                     /translation="MGKKIKITLVKSTIGRKPKHVAIAKQLGLGKTNSSVVHSDTPAI
                     RGLVNEINYLLLVEESA"
     gene            complement(17536..18042)
                     /gene="rpsE"
                     /locus_tag="U6309_000919"
     CDS             complement(17536..18042)
                     /gene="rpsE"
                     /locus_tag="U6309_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEN5504372.1"
                     /translation="MSFDELPKSDGYQEKLVSVTRTAKVVKGGRVFGFAVLVVVGDGK
                     GKVGFGRGKAREVPIAIQKAMDQAKKNMVYIPLSGTTIFHEITWNYGASKVFMKPASE
                     GTGIIAGGAMRAVLEVLGVQNILAKSIGSTNPSNIVRATIGALTHIGTPDYVAAKRGK
                     TVEEVMAG"
     gene            complement(18052..18411)
                     /gene="rplR"
                     /locus_tag="U6309_000920"
     CDS             complement(18052..18411)
                     /gene="rplR"
                     /locus_tag="U6309_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEN5504373.1"
                     /translation="MNKQNARHRRGLKAKALIRKTGRSRLVVYRSGVHIYSQIVKSDQ
                     LGDKVLVASSTIDKELRSSLTGKSKVEQASLVGQLLGKRAKAAGITQVAFDRAGYKYH
                     GRVKALAEGAREAGLDF"
     gene            complement(18422..18961)
                     /gene="rplF"
                     /locus_tag="U6309_000921"
     CDS             complement(18422..18961)
                     /gene="rplF"
                     /locus_tag="U6309_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEN5504374.1"
                     /translation="MSRVAKAPVIHSANVEVTFVDGVITVKGPKGILTQKINKLVNIQ
                     HSKESNKLEFSPASNDPMGWAQAGTARALVRNMVQGVTEGYTVTLELVGVGYRAQSKD
                     KSISLSLGYSHSIEYDLPKGVTVETPNNTTILLKGVDKQVLGQIASEIRAFRPPEPYK
                     GKGVKYAGEQIVRKEAKKK"
     gene            complement(18979..19368)
                     /gene="rpsH"
                     /locus_tag="U6309_000922"
     CDS             complement(18979..19368)
                     /gene="rpsH"
                     /locus_tag="U6309_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEN5504375.1"
                     /translation="MHDPIADMLTRIRNGQQAKHQQVTLVSSKLKEEIARVLKEEGYI
                     QDFFIETLPNGLKSITLKLKYYHGRPVIEFIKRISRPGLRVYKSYKDLHSIPGFGVAI
                     LSTSKGIMTHVSAKVKGVGGEVICEVA"
     gene            complement(19397..19699)
                     /gene="rpsN"
                     /locus_tag="U6309_000923"
     CDS             complement(19397..19699)
                     /gene="rpsN"
                     /locus_tag="U6309_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN5504376.1"
                     /translation="MAKKSMLMRESKRAKLVEKYRQRRNELKQLIKSSDDFQVIMESQ
                     AKLAKLPVNSNPVRYVTRCKQCGRPHAVYRKFNLCRICLRQQLMVGNIPGGRKSSW"
     gene            complement(19712..20263)
                     /gene="rplE"
                     /locus_tag="U6309_000924"
     CDS             complement(19712..20263)
                     /gene="rplE"
                     /locus_tag="U6309_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEN5504377.1"
                     /translation="MARLKEFYKKDVVTMMMKRFNYSSVMEVPRILKITLNMGVGEAV
                     GDKKVMNHAIEDMTLISGQKPVVTKARKSIAGFKIREGWPIGCKVTLRRERMYEFLDR
                     LISITLPRVRDFRGLNPKSFDGTGNYSMGIHEQIVFPEIDYDKTDGIRGLDICITTSA
                     KTNEEAKALLEAFNLPLKDKDRK"
     gene            complement(20279..20608)
                     /gene="rplX"
                     /locus_tag="U6309_000925"
     CDS             complement(20279..20608)
                     /gene="rplX"
                     /locus_tag="U6309_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEN5504378.1"
                     /translation="MKRIKSGDEVIVIAGKSKGHIGKVLRVIDDAVVVEGGNLIKKHI
                     KPNPQKPENKGGIIAREAPLHVSNVAHYNPVTKKADKVGFKYLESNGVSKKVRYYKSN
                     NEIIDRI"
     gene            complement(20621..20986)
                     /gene="rplN"
                     /locus_tag="U6309_000926"
     CDS             complement(20621..20986)
                     /gene="rplN"
                     /locus_tag="U6309_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEN5504379.1"
                     /translation="MIQMQTVLEVADNSGARKVMCIKVLGGSHRRYARVGDVIKVSVK
                     DAIPRSKVKKGAVMRAVVVRTAQGVRRDDGSLIRFDDNAAVLLNNQNEPIGTRIFGPV
                     TRELRERFMKIISLAAEVL"
     gene            complement(21075..21329)
                     /gene="rpsQ"
                     /locus_tag="U6309_000927"
     CDS             complement(21075..21329)
                     /gene="rpsQ"
                     /locus_tag="U6309_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEN5504380.1"
                     /translation="MSTNSESNARTMIGKVVSDKMDKTIVVMIERTVKHPKYGKIMKR
                     RTKLHAHDENQVCRVGNTVKIRESRPLSKTKSWVLVEVIS"
     gene            complement(21331..21525)
                     /gene="rpmC"
                     /locus_tag="U6309_000928"
     CDS             complement(21331..21525)
                     /gene="rpmC"
                     /locus_tag="U6309_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEN5504381.1"
                     /translation="MKKIDELRNMSVEELQNELLSLRKDQFNLRMKKASGSLDKTHLI
                     TMVRKSVAKVKTILTEKAGK"
     gene            complement(21525..21938)
                     /gene="rplP"
                     /locus_tag="U6309_000929"
     CDS             complement(21525..21938)
                     /gene="rplP"
                     /locus_tag="U6309_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEN5504382.1"
                     /translation="MLQPKRTKYRKQMKGRNRGLALRGSKISFGEFGLKAVERGRLTA
                     RQIEAARRAMTRHIKRGGKIWIRVFPDKPITQKPLEVRQGKGKGSVEYWVAQIQPGKV
                     LFEMEGVSKELAMEAFDLAKAKLPFKVMFEERTVM"
     gene            complement(21955..22611)
                     /gene="rpsC"
                     /locus_tag="U6309_000930"
     CDS             complement(21955..22611)
                     /gene="rpsC"
                     /locus_tag="U6309_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEN5504383.1"
                     /translation="MGQKVNPIGIRLGIIKDWNSKWFAGKRYAEFLIQDIKLRNDLKK
                     KLMAAAVSKILIERPANNAVVTILTARPGVIIGKKGGGIETLRKEISDNLGVPVHLNI
                     EEVKKPELDATLVAEGIAQQLEQRVMFRRAMKRAVTSALKAGAKGIKICVSGRLGGAE
                     IARSEWYREGRVPLHTFRADIDYGTAESKTTYGIIGVKVWIFKGEILPQKKRSTESAQ
                     "
     gene            complement(22614..22949)
                     /gene="rplV"
                     /locus_tag="U6309_000931"
     CDS             complement(22614..22949)
                     /gene="rplV"
                     /locus_tag="U6309_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEN5504384.1"
                     /translation="MEVTAKLKGAPLSAQKGRLVADMIRNMNVSGALDVLKFTPKKGA
                     KLMLKLLESAIANAENNNGADIDDLKVGMVCVDEATTLKRISPRAKGRANRICKRTCH
                     ITIKVSDEE"
     gene            complement(22959..23237)
                     /gene="rpsS"
                     /locus_tag="U6309_000932"
     CDS             complement(22959..23237)
                     /gene="rpsS"
                     /locus_tag="U6309_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEN5504385.1"
                     /translation="MARSIRKGPFIDHHLISKVEAAIESKSKKPIKTWSRRSTIVPEM
                     IDLTIAVHNGKDHVPVFITDNMVGHKLGEFAMTRTFKGHSGDRKAKGK"
     gene            complement(23256..24083)
                     /gene="rplB"
                     /locus_tag="U6309_000933"
     CDS             complement(23256..24083)
                     /gene="rplB"
                     /locus_tag="U6309_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEN5504386.1"
                     /translation="MALLKSKPTSPGKRGEIRVVHHDIYKGKPHAALVEKLKKTGGRN
                     NQGRITVRHIGGGQRQKYRIIDFKRNKDGILGRVERLEYDPNRTALIALISYKDGEKR
                     YIIAPSNLEVGATIQSGADSPISVGNCLPLKNIPVGTTIHCVEMKPGKGAQMLRSAGC
                     SGQLVAKEGVYATLRLRSGEMRKIHVLCRAVIGEVSNSEHNLRALGKAGAKRWRGIRP
                     TVRGVAMNPVDHPHGGGEGRTSGGRHPVSPWGLPTKGYKTRSNKRTDTFIVRGRKKK"
     gene            complement(24095..24391)
                     /gene="rplW"
                     /locus_tag="U6309_000934"
     CDS             complement(24095..24391)
                     /gene="rplW"
                     /locus_tag="U6309_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEN5504387.1"
                     /translation="MNAERLMMVLREPHTSEKATVMADKFKQFTFKVLKNATKTEIKL
                     AVEHIFNVKVKSVSVVNVKGKSKRFKQTSGKRSDWKKAFVSLHADQDIDFTVTE"
     gene            complement(24388..24993)
                     /gene="rplD"
                     /locus_tag="U6309_000935"
     CDS             complement(24388..24993)
                     /gene="rplD"
                     /locus_tag="U6309_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEN5504388.1"
                     /translation="MEITTIDTKSKLKLNKEIFAYTYNEGLVHQAVVAFMNNARSGNS
                     AQKTRSEVSGGGKKPWNQKGTGRARAGTIRSPLWRSGGVTFASKKRDYSQKLNKKMYK
                     RALRSIISELCRTGNLVVVSDFQCDNHKTKDFLKKMNQMEISNALIIMSEVGENEYLG
                     SRNLIDYDICDVTTIDPVSLLRFEKVVVTEAAIKKIEEQLQ"
     gene            complement(24993..25643)
                     /gene="rplC"
                     /locus_tag="U6309_000936"
     CDS             complement(24993..25643)
                     /gene="rplC"
                     /locus_tag="U6309_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEN5504389.1"
                     /translation="MMIGLLGRKIGMTRVFTPEGVSVPVSVVEVQPNRVSQVKTAAND
                     GYSAVQLTGGTKKSSKVSKPVAGHFAKAQIDAGDMQVEFRIDSEDAFTPGQVISVADV
                     FTAGQYVDVSGLTKGKGFAGTVKRHNFRTQDASHGNSRSHRVPGSIGQNQTPGRVFKG
                     KKMAGHMGNARCTIQSLELVKVDSERNLLLIKGAIPGAPGSRVEIKPAVKKQARGE"
     gene            complement(25678..25995)
                     /gene="rpsJ"
                     /locus_tag="U6309_000937"
     CDS             complement(25678..25995)
                     /gene="rpsJ"
                     /locus_tag="U6309_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEN5504390.1"
                     /translation="MSSNQNIKIRLKSFDHRLIDLSTREIVDTAKRTGAQIRGPIPLP
                     IRKEKFTVLTSPHVNKDARDQYELRTHKRLVVIVHPTEKTVDALMKLDLAAGVDVQIS
                     LDD"
BASE COUNT         7818 a         5667 c         4224 g         8288 t
ORIGIN      
        1 cttgatgtgt tgcatcaatt gatcatggat aatcaacact ctttggaaga gatactcgag
       61 gcatccaata atagatttct ctcttttcag acacgccata cctaagcact acttcactaa
      121 agaaagacac aaaatatgga agaagctatc tgcctgtctt actcaagctt aaaatattcc
      181 catataaaat aacaagaaga atgaatcatt gagtttcaaa gcaaatctca tataattaaa
      241 atttaactct ataataatac ctcatgagac acatttattt tgcattatta ctcatcgttg
      301 tcacccagac ttctttgtgt ttgtcagctt gtgggcaaaa aggacctttg tatttgcctg
      361 aacaagaaaa gaaagcacca accgatcaat agagacatcc tatgggaata aaatttacca
      421 aaatgcatgg tttaggcaat gacttcattg tattggatgg agtcaaccaa tctattcaac
      481 tcactgttga gcaaatacag aaactagcca atcgccatac cggtataggg tttgaccaat
      541 gtttactaat agaatccagt caaaccgaag gaattgattt taattatcga atcttcaacg
      601 ccgatgggca agaagtaggt caatgtggta atggggcgcg atgcatagct ctttttgcca
      661 gatattatgg tttaacagca aaaaataaat tgacagtagc aactaaaact acgctcatgg
      721 atttaatcat caatgaggat aacagcgtga gtgtcaatat gggcgttccc agactagccc
      781 caagtgaaat tcctttactt gctgatcgcc aatccccaga atattcactt gaattaaata
      841 acggcaatac agtgaatctt catgcgataa gtgtaggcaa ccctcatgct gtattgttgg
      901 ttgaaaatat agatacagcc cctgtaaaca gtttgggaca acagattagt tttcacccgc
      961 aattcccaga gcaagtcaac gttggattta tgcaaattgt taatcatgaa aaaatcaact
     1021 tacgtgtcta cgaaaggggt tgtggtgaaa cgattgcttg tggtagtggt gcggttgctg
     1081 cggcagcgat agcacgttta ttttacaacc tgtcagacaa aatcaccgtt catttgccag
     1141 gaggagattt gtgtatccaa tggccttgtc ccactactcc cattatatta acaggacctg
     1201 ctgcttttgt gtatgagggc acattacttt cataacaaaa ttcacaaaat taaaacaagt
     1261 attgaatttt tttttcaaac agttattctg aattaatcca caaatataaa acaggactta
     1321 ggaaaaacgc taaggtcaag gcaaaaaaat ttttaatgag ggagtttaga taaactaaat
     1381 ggccgaatta aaaactggtt tttacgaaaa gattcggggg ttgcttaagt cctgtaaaga
     1441 ttccatcggg agaatttgtg aatattatat tccgtggctt aacatgaacg aaatgggtat
     1501 taaagttgat agagtagttt tgacatttcg gtcaaggagg atcaagtggt tgattttgat
     1561 aaacctgtct caaaaaatga tattcttgcc agcttattac ttatcgcaga tttaaataga
     1621 ccatctctaa atcaacctct ttcttgggag caagctctaa aatcattttt atcgcttaat
     1681 gatccactga ttaaaacgga aatatttata aaatggctca gtcaattaaa tcctgatcaa
     1741 cttcaatcgt ccccagagat agtcctcctt ttgaaacaca ttgtcgagca aaagctgctc
     1801 ccctacaccc tcaaaaaaat attgattcag cgtgaaaact tcaccacctc acaaatcaat
     1861 tggctttgca atcattgtct tatagttgca cctgatcaaa cgactttacc tgatattatt
     1921 aaactcggct catggagctg gctggagaat catatgaaaa gaaatgaact attaaatttt
     1981 gatttgttaa aaatagccat cttaaataag gattatttag ccagcatcaa caatagtaaa
     2041 gaaagtcagc ttgattttat ctatgcgtta aacgctaaca aatttaacta ccaacaaata
     2101 cttgaattaa ttgctttagc gcaggatagc cagattaaat cattattaat tacctgtcta
     2161 ttaagcaaaa aggagtacct ggacaattta aaagaaaaat catttctagc ccatttaaat
     2221 caaagtgaat ttcactcgcc acctcggctt aatgcactaa ttcaccaatt aaacctgaat
     2281 tgtttgactg tggcacaaat agaacttcta caacctgaag catgtgttac tttactctgt
     2341 tcgatccctc attttcatca attgtcagaa aaacaggttg aaaaaatact acttaaatat
     2401 ccaaagcctg atatgattgc ctattggcta aatcattact catccaggcc aaatgcgcat
     2461 tttattttag cccatttgat gaacctggta gatgttcatg ttgttgacga acttaataaa
     2521 atggattcca caaaaaagga agccattatt atgaatatgg tggaacatct cgaattattt
     2581 gatccgactc atcctctttt gatcaaccaa aatgaagaaa aacgattaat taccgctatt
     2641 cagcgctatc tctatggcca acatcatgac gcgtatgtaa cttacatcaa gttattggtt
     2701 tataaattat taaaccgtaa ttaccaattc tcacctcaag cgatgcaact cctgatttgt
     2761 ttaaataatc tggaagagtt taaagagtta actaataaaa tcgcctatct gaccaattac
     2821 tacttgcgag cattagcaca atccggtgat tcgagcttat tttataatga ttcacgcata
     2881 aacatcacga gcatgacaca actggtccag ctagccccaa aaattccaac taaatcaaaa
     2941 gaaaaaagtc ttttaagcag cctcttctgc ggcatcaaaa ttaacgaaag ctctgtaatg
     3001 acaccgacca atgatatacc tgaacaccct ttaattaata aattaataaa aagcaagaga
     3061 acggtgaaat ccatagatta ttttttaatg cattataaag gcgacaagca agtactttcc
     3121 aaaatcatca atgattattt aagttactat gttcaagaag ggtgcactga aatacatcgg
     3181 cgttcgatat atcatttaat tgttctcatg tctttaagcg atatatcagc ttctgttaga
     3241 gaagctatat acacagcgtt tctaaactat cctgaactac atgatgagca aattaatttt
     3301 tctttattta tctatgatgc taaaagaact atccggcatt ttggggcaca agctagcgaa
     3361 gacagctatg ccagagttat tgatttatgc gagggagctc taaaaaaatt aaatccaaat
     3421 aaacacaagg aaattatcag tatagcgact caagctttaa ctgaagcaaa aatcgagtta
     3481 agttttggtg aaaataccgg atttatcgct caactgattt cacgattgaa acgatgttgg
     3541 tattatggct ggactggttt tttttcccca aatccaccta cttttgttgc agcaattgac
     3601 tcgccaataa agcaaagcga aaaaaaaacc tcccctcttc ttcaacaaag aaaatatttc
     3661 actaccaaac ttgaaacaaa tctgtcgatt atcctcgatg acataaaatc aggttacacc
     3721 caacaaaaac ttaatgaatt aatcgaagca ttgagtatat tttcattgca atccaatccc
     3781 aaaaacgagc ttaaaattcg tcaaaaactc catcgcttat ttcactatct gttactagaa
     3841 agcaaaaatg acttgacatt ggatgcctgg cttataaaaa atcagcaact tttaatcgca
     3901 aatcgttttc gtttgctaga aatgcaacta atgaaagggg ctcgtaatga ggttgaatct
     3961 ttacttaagg aattcgatga agactctact catttcagcc ctatcagtaa agagtttact
     4021 cattctttac ctgaacaaaa cccgtataag tgtgaatctg tttcttcaaa tcaaactcca
     4081 ggcgcaacta ttctggattc agcaactgag ttagctacta gcttaaaaag cacattaggc
     4141 aacttgggag tattttttag caacaacatc atgtcagcta tttatcccaa taataaatcc
     4201 actccccaat cgacatcaac aatgatttca ccttgattaa tcttccggtt gcgtatctgc
     4261 agccggattg attcatctat ccgttataat gacctgaaac aaacaaccgt tctaaaattt
     4321 agatcctact tacttaatca gcagatatca cactcatcag gttgacctat caggaataat
     4381 agacctaaaa ttaaaaagca tacttaattt atggttaaac aatggacgaa aggcgaaaat
     4441 attttcggct aaaaaaccat ggcgaaataa atgccagctt agacaataat ccaattgaga
     4501 ttgttgaaat ctcttccaat ggtgctgtag tagttaagca gaaaactgat attcctaaag
     4561 ggggaatgct taaactacaa atccataact tcattatgga actttgctat gaagtgattc
     4621 gagctgaaga cagcaatatt gtccttcatt tcacaaaaga agacgaaacg aataagttat
     4681 ttctggtact caaacgatta agagacgaac gaaaaaaata aaatataaaa atttgacata
     4741 acttgcccca taaaaagtca tttgtgatta agtaggagcc tggagaaaag attctgtgac
     4801 aaagaataaa taagaaaccg aagacaatcc attcatttaa gaaagtttcc agcgtttgtg
     4861 catccaattc cactgctccg gatgtgccag aataatcttc tccaaagcct gattataggc
     4921 caaggtattt cggtataaag attcttgcgt agtgtcataa tctttccaag gaatgggatc
     4981 atgaaactcc aaaacatgct ttccgttagg taatctgtag ctggctgcag gtataactgg
     5041 aattcctgta tggcgtgata tcgtcgccag gcttctgtag gtgcccgctt ttttcccaaa
     5101 aaattctacc gctatcccat ctctgttcac cagggatgca tgctggtcca atacgaagat
     5161 tacggcatga ttttgttcca acgcaacaca aacctgctcc agagaatttt ttttaggaat
     5221 aacatttagg ccagcttgat agtaattttt gaacataatg cgttctatga acttaaatcg
     5281 caaggtacgc ctgatgaaat gaaattggcc tttgaattcc ttgaagttta atacccctcc
     5341 caagggcgca aactcccaat ttccaaagtg accggtaact actaacactc cttttttctg
     5401 tgccacaaca gctaacattt tttcatggcc gattacttca acctgagctc gtaatttttt
     5461 ttcactcatg aatcttaatt gcagcgcttc ttttaaagat tttgcaagat gtgagtaata
     5521 cgcttttacc aagcgctttt tttgagattc attcagttga tcattataaa cctggctaat
     5581 attggaaaaa atcaaccggc gcctgtatgg caaaaaccga taaataaaac gtcccgccag
     5641 tgtaatgggt aatgcttcta tttgttgagc taaattattc acaagctttg atcaccaaac
     5701 atacattcat gctggcaaac cctcggttga tcatcatggc ataggaatta gatattatct
     5761 ctctatgctc acgacttact aataagcctt cacacgtcgg ggcaatttgt tgaaaattag
     5821 cgatccccgg cacgcatttg gtttcaaggg catatgtagt taataccgca tccaatactc
     5881 cagaggcaca taaagtatgt cccatggacc atttaaaagc agtcgcagga acttcttctt
     5941 gtttgaatac tgatttgata gccagagcct cactgtcatc tgatttactg ttcccatttc
     6001 catgggttac tattaatcct atttcctcag agtttattcc tgatgtgctt aacgtctgcc
     6061 ccatcaatga agccaaatgt tgcccatctg cttcaataga aaataatcct gttgcttcag
     6121 aagcggataa gccaccaact atctcaccat aacaagtcgc agccctggac ttcacactgg
     6181 cttcactttc caatacaaga gccgcggcac cttcagcaag aatggtaccg tcatgctctt
     6241 tatcaaaagg ttttaaatga cgattactta ccacacccaa tttttcataa taaaataagc
     6301 cttgaggttc tgtgcctata tcataggcaa ccacaaccgc gcgttctgcc tgcccgctac
     6361 gtatggcatg atacgcttcc aaaatggctt gtgtaccacc taccgcatga ttcgttacat
     6421 tgtgattagg acctttaaag ccataggtaa tacctgtata ggcaagcaca ttattaggta
     6481 aaatcctcaa taaccacata ggatgcactt catcaaacaa ctgatttgca aaactacgca
     6541 tatctccatg ggttttagcc aacaaaggta taaagtcata ttgttgaaaa tatttattcc
     6601 ctggagaacc aacataaata gcggtttgtt cattaaataa atcagcatct gccaggctat
     6661 ctctgtaagc aatcatttta ctatgctcaa cagcttgcac tgccgcattg attcccatta
     6721 tatcctgacg ggaaataact tttattaatt ttctatcagg aagcatcttg gaaggttgga
     6781 aatcttttat ttctccgccc aaacgatgcg accattcaga taattcccaa aaattaattt
     6841 catctattcc acactggcct gaaatgattg cattccaagt ttcatcagca gttgcaccag
     6901 aggcagttag agcgctgcga ccagtaataa atactctatt cgcttgactc atttattttg
     6961 cctcctcgaa ttctggatgt ttaaataaca agcagctatt agaaccccca aaaccaaacg
     7021 aattagataa tactacccct aaagattttt cacgtggacc atcaacaaca taatccaaat
     7081 cacactccgg atcaggtgtt tttaaattcg ctgttttggg gacttgcgcc tgttctatgg
     7141 atttaactga caataccgct tcaagcgctc ccgcagctgc gattaaatgc cccgtttgac
     7201 ttttggtaga gctaactggt aaattggcga tcctgtcccc aaagacaact ttgattgcat
     7261 tggtttcact taaatcattc attttagtgg atgtaccatg cgcattaata taatcgacat
     7321 cccttggttg aacacccgca tcctccaacg cacgtttaat cgcttgaata gcaccatcac
     7381 cattgggatg tgaatcagtt attcggtagg agctcaaaga attcccctct ccggccagtt
     7441 cagcatagat ttttgcccca cgggcttttg ctttactcca ttcttcaagg atcaaaaagg
     7501 cagccccttc acccaggaca aaaccattgc gtgtcgcatc aaaggggcga cttgccgttt
     7561 gcggagtctc attataagtt gataaagctc ccaaaagaca aaagctcgac agacccaaag
     7621 gattaatcat ggaatcaaat ccacctgcca acataaaatc tgcctcgcca cgacgaatca
     7681 cctgcattgc caatcccaag gcttggcctc ctgaagcaca ggccgtatgg acagaagagg
     7741 cataaccagt aatcccatat tgttgtatta ataaggaaag accagagttt gaagtggttt
     7801 tgccaaaatc aaccaaatta tagaaatccc tgctattaga ttgcagcttt ccccaatcaa
     7861 tttcaccgcc tgcagcacaa gtttgttgaa accgcgttaa atactcaaac tcggccgtca
     7921 tcatcccact gcctgttacg attccccacc gatcggatgt ttgcttggta gggaagatag
     7981 aagcatcctc aaaagcctgg cgtgcagctt ccaatgcaaa atgagtaaaa gacatgataa
     8041 atcgagtatg ctttcccgaa atagacgtat caggtgaaaa attctttact tcagcagcaa
     8101 tagtggtagg aaaagcactg gcatcaaact gcttgatact atctatgcca gaatggccag
     8161 cgatcaaatt cttccaagtg gtggatacat catttcctag aggcgaaaca acccctagac
     8221 ctgtaatagc aactcgtcgt ttcataacct catttacctt ttgggataag cactatgtcg
     8281 acaacacata cccgcttgcc atcaacctca cacctataag acagtattaa ttcatcatcc
     8341 atttgctgtt tcactttgac ctggcaaact acgacatctc cgggatgaac aaattcattc
     8401 attttaattt tacgtaattc gcttattcga taagctctat cgtattttgc tctagctata
     8461 aactctttcg ccaaattcaa tttacattca agaagtaccg tcatcggtag tacaggtcta
     8521 ttaggaaaat gatccgggaa ataagatgct gaacgagtta tccttttttc agcaatcaag
     8581 cttatacctg gttcactggc gaggattcgg tcaaattgca tggaagaatt aatcattcca
     8641 caatcagagc agttttcctc tattaaaaca ggcgaactca aagctaacac agaagaccac
     8701 tcacctggcc ggtatatctc tgcaaactgc tgtctaatga cctctgtact aataaattca
     8761 gtcataggca gcatggggcc taatgctcca tctatagtaa aaataatatc attacctact
     8821 ttggcaatgc tatggtattg aactgccttt tcatccagtg aatcaatatt tgattcaagc
     8881 aatatcgttt caccgacgta agcaggtcga tacaatcggg cacttgatac tacgccggcg
     8941 actggtcttg aagtaaaatg attatgttgc atcacattcc acgctgccaa ttgccccaat
     9001 gtttcaccaa tcaaagaagg cataaagcaa agccttccat gatcatcagt ggttaaataa
     9061 gcatcatctc ttgtgacatg ctttatccct ttaatccatt ctcctgggga taattgtaca
     9121 atgcgatcaa caaataagaa cctcatcagg ctacagtttc cgttgcttgc tgctgggcta
     9181 tagctgaaat aaccagcttg caaaaagttt ctacggtaaa caacacagga atttcgttaa
     9241 ctttcatatt ctctttaaat cgatcagcag gaacttcgct caaataattc tttaaagcct
     9301 gtaatcctga ggttgttaaa ataccacctt tctcaaactc atcttcagcc agatcaccac
     9361 gagcattctt ttccaactgg cctctcggga ttttaatctt gaattctttt tccaattgaa
     9421 aaaccaaatc aagaaaatcg atggactcag cacccaaatc ttcaattaaa ctactgttta
     9481 aagaaatttc ttcttcatca attactaata catcagcaat aatttcccta accttagggt
     9541 aaacttcagc tacattcata ttttatcctc tgaaccaaca aagctcgcaa agcttgaaat
     9601 tatatcataa aatttacttt tatcatctac tacccaacct gactatagcc tgcgtcaaca
     9661 tataatgtat gagcattgat catggccgct tcaggcaaac aaagcaaata gatagcatta
     9721 gccacatctt caggtgttaa cgctctatct gccaaagcat gagcttgata ttcatccagt
     9781 aactgttctc tgtttgggaa atgctttaaa gcgtccgtat cgacaacacc tgcagaaaca
     9841 gtattcacag taataccata actagccagc tccaaactta gcgaacgaac caatgcttcc
     9901 agagctgctt tagaggcgcc aataaaagca taatttggaa tcgcccttga tgcccctaag
     9961 ctggatagtg caataactcg actgttttta ggcattaagt caagaccttc agtaaccaag
    10021 tggttcaaag ccaaagcatt ggtttccaaa caccagcgcc aatgcttggt cgacattttc
    10081 aaagcaggtt ttaatactcc actcgctgca ttactcacta gaaaatttaa gtgcttaaag
    10141 tgttctctaa attgagagaa catttctttg acagattgac aatctgctac atcggcttgt
    10201 aaggcaactg cttttctacc catgccggtt atttcattga ccagactttg agcctcatca
    10261 gagctattat aatacacgat agcaatatcg cagcctgaat gtgctaactt taatgcggtc
    10321 gctttaccaa ttcctcgtgc accacccgtt atcaatccta cctttcctga aaaaactaat
    10381 gaactcatac gataacccta tagtataaaa tcccaccaaa ctaccgctaa tctgctgtgc
    10441 tgtcaatgga aatatcggga ctctataaag cggaatttca taatttatct tgtttccaaa
    10501 gcacttaatt tgtaccatct ccacatgata taatttagac atataatgaa tgaagtattc
    10561 ttggaacaaa actgtttttc ctatagcagc aatattctct tttcgtctcc tgggattatt
    10621 tttacttatc cctgtattca ctctttatgc cactaatctt aaaggtgcta ctccaacatt
    10681 aattggcttg gctttgggaa gctatggatt ggcacaagga ttactacaaa tcccttttgg
    10741 tatgctttca gataaactag gcagaaaacc tatgatcgcc ataggtctat tgctattcgc
    10801 tggaggaagt ttgttaggag cagtaactca ttctattgct ggaatgataa ctgccagaat
    10861 actacagggt gctggagcta taggtagcgt gttaattgct ttgcttgctg atttaaccct
    10921 ggatgaacaa cgtacaaagg caatggccat agtagggata actataggaa catcatttag
    10981 cctcgcaatg ataattagcc ctgctcttac ccactactat ggattatcag gagtgttcta
    11041 tctcactgca gctttagctg ttctagggat tataatactt tatctcatca ttcccaatcc
    11101 ttcaaaagag cagtttcatg cagatagtga acccagcttg tctctcataa aacctgtggt
    11161 aacagatcaa catttacaac gcctcaattt tggaattttt tgtcagcatt ttattcttac
    11221 agccactttt tttgctatcc ccatgctgtt gaaatatcaa attgagaatg gtcatttgca
    11281 tcagcaatgg cacttctatt tgccactcat gttattctca tttattttaa tggtaccctt
    11341 tatcattatt tcagaaagaa aaaaacgatt aaaaacagta tttgttatat ctgtactgct
    11401 gactactttt actcaaggtc tgttggtgct cacatcacaa aattggtatt ggttttgtat
    11461 tttaatgttt gtgtatttcg tttccttcaa tgttcttgaa gcaacattac cttcattagt
    11521 gtcaaagctg gcaagtccag ggagcaaagg cacagcaatg ggtatttatt caacgagtca
    11581 atttttagga attttttttg gtgggtcttt agctggtatt ctttatcagt ggtacgatag
    11641 ccaagctatt tttctaatta acatgattct gagctgtcta tggctcatca tttcgctgag
    11701 catgaaatct aatgtttatt tttctactct tattttgcct tttacagcaa ataatgaaga
    11761 atcaaaatta gtagtcaatc agcttttaaa ggtaaccggc attaaagaag ttattttgtc
    11821 caaggatgaa aataccttgt atattcgagt agataatgag gaatactcca caggaagcgc
    11881 tgaaaaaatt cttcatcagt ctactattta ccaataacac cttctcattg ggctgctacg
    11941 aaataactgc caaattatgt ataataccta actaactttc gcatacagga gatgatttca
    12001 atggcaagag gaataaataa agtaatactt gttggtaatg taggggcaga tcctgatgta
    12061 aggtatttgc ctaacggaaa tgctgttact acactttcag ttgctaccag tgaaacatgg
    12121 aaagacaaaa cgacaggtga aaaacaagat agaactgaat ggcacagagt tgtctgtttt
    12181 aatcgtctgg gtgaaatagc cggtgaatac attcgtaaag gttccaagct atatgtcgaa
    12241 gggagcttaa gaacaagaaa atggcaagat caacagggcc aagatagata tacaactgaa
    12301 attgttgcgt ctgacataca aatgctcgat agcaaaggaa gtagtgctac taattacgac
    12361 gacatgcctt catttcaggg aagctctacc cctcaacaag cttcaacaaa gaatcaagca
    12421 acaccaacat ctactgcaca agatgctttt gatgaattag atgatgacat accgttttaa
    12481 atgattcaaa taaaagcccg cgttaatgcg ggctcaaaat atagattata gttattaatc
    12541 taaaatttac tcttctgtat catcactgct tacaggtcta tccattaatt caactatagc
    12601 cataggtgca ttatcaccat ccctataccc acatttaata atacgtatgt acccacctgg
    12661 tcgctttgcg aaacgtgggc ctaaatcagt aaataattta cctacagctg atttagatcg
    12721 caaaatatta aaagctcgtc tacgtgatgc tactgaatca gatttgctaa cagtaatcaa
    12781 aggttcgata tatcgacgta aatcttttgc ctttggcaaa gtggttctaa ttaactcgtg
    12841 ctcaatcaaa gaacaacaca tattagaaaa catagcctta cggtggctac tggtacggct
    12901 aaaactacgt ccggaattac ggtgacgcat aacaaagact cctaaatatt actcgccaag
    12961 gcttgctggc ggccaattct caagtttcat acccagcgat aatgatcgag acgctaaaac
    13021 gtctttaatt tcagttagtg atttcttacc aagattaggt gtcttcaata attcactttc
    13081 tgttctttga accaaatcgc ctatataatg aatgttttca gcttttaaac aattagctga
    13141 tcgaacagtc aactccaaat catccactga acgtaataat acaggatcaa aatcattacg
    13201 ctctttattg tcagcacgtg attcttcaaa tttcatgtca acaaacgcat gaagctgtct
    13261 ttgtagtata ctagcagata ttcttattgc ctcttcagca tcgatagtac cgttagtttc
    13321 taattcaata gtaagcttat ccaaatctgt tctgttttct acacgtgcac tgtcgacgaa
    13381 ataagctact ttcttcactg gagaaaaact gttgtcaatt tttaactttc caaccgtctt
    13441 tttctcaatt tcatcatcat gatagcgaac aaatgcatca gtattatgaa agccaatacc
    13501 tctttcaact ttaagagtca tatttaactt acccttttca ttgagattgg ctatgacaag
    13561 ttctggatta ataatttctt gaccatgagt caactgaata tcaccagctg taacttgaca
    13621 tggtccttct ttatttaggg taacctgcgc ttcgtttcct acagtcaatt ttatagcgac
    13681 tgattttagg ttaagcaaca aatcaaccac atcttcctga acaccttcaa tagtactata
    13741 ttcatgcaaa acaccatcga tagaagcttc agtaatggca ctgcctggca tggatgacaa
    13801 taagattctt ctcaaagcat tgccgagggt gtgaccaaaa cctcgctcca aaggttctaa
    13861 aactattcta gctttatagg gcgactcggc ctgtactttg agaaccttag gcgtcaacat
    13921 ttcattgatt tcagtataca ttcagctatc tctccgagct tacttagagt aaagttctac
    13981 aacaaggttt acgttgtagt ctgaagataa atccattaac gttggtgctg tagaaaatgt
    14041 acctttaaaa gaaccagtat ctacagtaat ccaatcacag ggtgctcttt gttcagataa
    14101 agctaaagca gcttggatac gtccttggtt tttagccttt tgtcgaacgg atacagtatc
    14161 cccaggatta accaggaaag aaggtacgtt aactacttta tcatttacaa gtattgcctt
    14221 atgtgcaact agttgtctag cttcagcacg tgtactggca aaacccatac ggtaaacaac
    14281 attatctaaa cgtctttcca atagggccat taaattttca cctgtagaac ccttttgtct
    14341 agccgccttt ttgtaataat tacggaattg cttttcaaga atcccataaa gccttctaat
    14401 cttttgtttc tctctaagct gaattccata actatttaaa cgaggttttt tatcaccatg
    14461 ctgtccaggt aatttttcag atttacactt ggatttatga tcacgaaccc cacttttaag
    14521 taacaaatca caaccttcac gtcgtgataa tttacattta ggaccaagat atctagccat
    14581 taattactcc tgagtcttat acacgacgtt ttttaggtgg cttacaacca ttatgtggta
    14641 tgccagttac atcggtaatt tcaacaattt taaaatcctg ggttattaat tcacgaatag
    14701 tagattcacg accaggacca ggaccatgca caaaaactgc aacagatttc ataccatatt
    14761 ctttagcaac agcagcagct cgctcagttg caacttgcgc agcataagga gtactttttc
    14821 tagaacctct gaagccagat ccaccagaag ttgcccagca taatgcatta ccttgtctat
    14881 ctgtaaaggt aactatagta ttattgaaag atgcatgaat gtgtactatg ccatcagaca
    14941 ctactcgttt tacctttttt cgtgtttttt gctgctttga ctttgttatt gccatctttt
    15001 attttcctac atgaaaaatc aactactagt gccttttcta cggccttttc tggtacgagc
    15061 attggttttc gtacgttgtc cgcgcaacgg caatcctctt ctgtgtctta atcctctata
    15121 acaaccaaga tccattagtc tttttatgtt cattgttaca acacgtcgaa gatcaccctc
    15181 tacagtaatt ttcgcaattt cagttctcaa agattccaac tgagcatctg ataattgaga
    15241 aactttaact gaaggatcta tatcaactgt tttacataat tttaatgatg ttgtcttacc
    15301 aataccataa attgctgtta aagcgattac cacatgttta tgatcaggta tattaactcc
    15361 tgcaatacga gccattagtt tctccgaacg ttatttgttc tgtcgaaagg gacagaagga
    15421 tactatttta ataaaataaa atcaagcaga tattaccctt gcttttgttt atgacgggca
    15481 tccttacaaa ttactcgtat agtgccactt ctttttatta ttttacaatt gcgacatatt
    15541 cgctttactg atgctcttac tttcattcct aactccgata aatcatagca gaccaggtaa
    15601 cttagtacct ctaaaattcg ccttttttaa taaagaatca tattgctgag tcattaaatg
    15661 agcttgtacc tgtgctacaa aatccataat aacaacaact ataatcagca acgaagtacc
    15721 accaaaataa aatggaacat gccaggtata cattagaatt tgtggtaaca aacatactaa
    15781 aaccaagtaa atggctccaa ctaaagtcaa acgagtcatc actgaatcga tatacttggt
    15841 tgtttgctct cctggacgaa ttccaggaat atatgctcct gattttttca ggttatctgc
    15901 tgtatcctta gggttaaaca ctaaggcagc ataaaagaat gcaaagaaca aaatagctgc
    15961 tgcatacact atcaaatata agggttgacc tggtgatagt gccattccaa catcagataa
    16021 ccatcccagc cctttagttt gcgaaaaaaa ttgagctaaa gttgctggca ataaaataat
    16081 gctagatgca aatattggtg gaatcacacc tgacatattt atttttaatg gtaaatgact
    16141 cgtttgggct gcatatacct ttctaccttg cgtcctctgt gcataattaa cacgaattcg
    16201 tcgttgagca cgctccataa atacaacaaa accagttact acaattacta ctactgcaat
    16261 aataactagt gtcagggctt gcatctgacc ttccttaact tgctggagga cagatcctat
    16321 agcatttggc atactagaga caattccaga aaagataatt aaggaaattc cattaccaac
    16381 acctttttcg gtaatttgct cacccagcca cattaaaaac atagtacctg taactaaagt
    16441 tacaacagca gtaaaataga atgagaaatc tggctgcata gcaatttgct gactagccaa
    16501 ccatctcgcc atacccatgg actgaaatat tgacaagagc aatgttaaat atctggtata
    16561 ttgatttatt tttctacgcc ctgattctcc ctctttcttt agttgctcca gcttgggaga
    16621 aactacagaa aacagttgaa ttataattga tgcagaaata taaggcataa taccaatcgc
    16681 aaacactgtt acacgagaca gtgctccacc agaaaacata ttaaacaagc caaatattgt
    16741 gttttgctgt tcactaaaaa aattagctaa ttttgcagga tctaatcctg gaacaggtat
    16801 atgagcacct aatcggtaaa ctaatatgcc gagaacaaca aacaacagtc ttgactttag
    16861 ttcggctaat ccaccacgag gcttactacc gttatgcttt tggtttttca tagtcactct
    16921 tctatactac cgcctaaact ctcaatagca gaacgagctc ctttcgtgac tcttaaaccc
    16981 ttaagcttaa ttgctgcagt cagctcgcca gataaaatta ctttaacatc tttgattgag
    17041 ttattaatta aacctgcttt acgtaaagca actaagtcaa ttacttcatc attcaatttt
    17101 gcaagctcac ctaaactcac ttcatcaatg gttcgaccta ctctagactt gaaacccatc
    17161 ttgggtaacc gtctctgtat aggcatttga ccaccttcaa aatttatttt gtggtaacca
    17221 cctgctcttg atttttgacc tttatgacct ttgccacaag ttttgcctaa tccagaacca
    17281 atacctctcc ccactcgtct tcttgacggt ctggaacctg gatctggcga taatgtattt
    17341 aaattcatta tgcactctcc tcaaccaata acaggtaatt aatttcattt acaagtccac
    17401 gaattgcagg agtatcacta tgaaccacac ttgaatttgt tttacctaaa cccagctgtt
    17461 tggctatagc aacatgtttt ggttttctac ctatcgtgct tttaaccaag gttattttaa
    17521 ttttttttcc cataattagc ctgccattac ttcttcaact gtttttccac gtttggctgc
    17581 tacatagtcc ggcgtaccaa tgtgtgtcaa tgcaccaata gtggcacgaa caatgttact
    17641 tggattggta gaaccaatgc tttttgcaag aatattttgc acacccaaca cttccaaaac
    17701 ggcacgcata gcgcccccag caataattcc tgtaccttca cttgctggct tcataaacac
    17761 tttggaagct ccgtagttcc atgtgatttc gtggaatatt gtagtacctg aaagaggaat
    17821 gtaaaccata tttttcttgg cttggtccat agctttttga attgctattg gaacctctct
    17881 tgccttacct cgtccaaaac ctactttacc ctttccgtca cctacaacta ctaaaacagc
    17941 aaaaccaaaa actcgcccac cctttacaac tttagccgta cgagttaccg ataccaactt
    18001 ttcttggtat ccatctgact tgggtaattc atcgaatgac ataatcgttc cttaaaaatc
    18061 taatccggct tcgcgtgcac cttcagcaag tgctttaact ctgccatgat atttataacc
    18121 tgctctgtca aatgcaacct gtgtaatgcc tgcagcctta gcacgcttac ccaatagttg
    18181 accaactaaa ctggcttgtt caactttact ttttccagtc aaactggatc tcaattcttt
    18241 atctatagta gatgatgcta ccaacacctt atcaccaagc tggtctgact tgacaatttg
    18301 agaataaata tgcacaccac ttcgatacac aactaatctt gagcggcctg ttttgcgtat
    18361 taaggctttg gcttttaatc cacgtctatg tcgtgcgttt tgcttattca tagttaacac
    18421 cttatttctt cttagcttct ttacgaacaa tttgctctcc agcatatttt acacctttcc
    18481 ctttataagg ctctggtggt cgaaatgctc taatttcaga agctatttgt ccaagaactt
    18541 gcttatcaac gcccttcagc aaaatggttg tgttattagg agtttctact gttacacctt
    18601 ttggtaaatc atactctata gaatgcgagt atcctaatga caaactaatg gacttgtctt
    18661 tagattgcgc tctatatcct acaccaacca attctagtgt aactgtatat ccctcggtaa
    18721 ctccttgaac catgtttcta actaaggctc tagctgtacc agcttgtgcc catcccattg
    18781 ggtcattaga agcaggtgaa aattctaatt tgtttgattc cttactatgt tgaatattaa
    18841 ccaatttatt aattttttga gttaatattc ctttggggcc ttttacagtt attactccat
    18901 caacaaatgt tacttcgaca tttgctgaat gaattactgg ggcctttgct actctagaca
    18961 tatcattcct cgcaagtatt aagccacttc acaaatgact tcgccaccaa caccttttac
    19021 ttttgctgat acatgggtca ttataccttt agacgtagaa agaatagcca caccaaatcc
    19081 agggattgaa tgcaaatcct tatatgactt atatactctc aaacctggac gactaatgcg
    19141 cttaataaat tcaattaccg gtcttccatg atagtacttt agctttaaag taattgactt
    19201 cagaccattt ggtaatgttt cgataaaaaa gtcttgtata tatccttctt cctttaaaac
    19261 tcgagctatt tcttccttta atttagaaga aaccaatgtt acttgctgat gtttagcttg
    19321 ttgaccattt ctaattctag tcagcatatc agcaattgga tcatgcatac tcacaattat
    19381 tctccaataa aatagtttac cagcttgatt ttctaccgcc agggatattg cccaccataa
    19441 gctgttgtct taaacaaatt ctgcaaagat taaacttgcg ataaactgcg tgtggtcttc
    19501 cacattgctt gcatcgagta acatagcgaa caggatttga attaactggt aattttgcta
    19561 gttttgcctg actttccatt attacctgga aatcatcaga tgatttaatc aattgtttta
    19621 actcgtttcg gcgctgtctg tacttttcta ccagcttcgc acgctttgac tctcgcatca
    19681 gcattgattt tttagccaca atttcaccct tttattttct atctttgtct ttcagtggaa
    19741 gattaaatgc ttctaacaaa gctttagctt cttcatttgt tttagcactt gttgtaatac
    19801 aaatatctaa accgcgtatt ccgtctgttt tatcataatc aatctctgga aacacgattt
    19861 gctcatgtat tcccatgctg tagtttcctg ttccatcaaa agattttgga ttcaaaccac
    19921 gaaaatctct tacacgaggt aaagtaatag atattaatct atctagaaat tcatacatac
    19981 gttcacgtct gagcgttacc ttacaaccaa ttggccatcc ctctcttatt ttaaaaccag
    20041 ctattgattt tctagctttt gtaactactg gtttctgacc agaaatcagc gtcatgtctt
    20101 caatcgcatg attcatgact tttttgtcac caacagcttc tccaactccc atattcagag
    20161 ttattttgag aatcctgggt acttccatta cactcgaata gttgaaccgt ttcatcatca
    20221 tagtgacgac gtctttctta taaaattctt taagtctcgc catttcaatc acctaactct
    20281 atatacggtc aattatttca ttgttagact tataatatct aacctttttg ctaacaccat
    20341 tactctcaag atatttaaag ccgaccttat cagccttttt tgtaacagga ttatagtgag
    20401 caacatttga aacgtgcaat ggagcttcac gcgctataat accaccctta ttttcaggct
    20461 tttgtggatt aggcttgata tgctttttaa ttagatttcc gccttctact acaacggcat
    20521 catcaattac ccttaaaacc ttacctatat gacctttact tttaccggca attacaatta
    20581 cttcatcgcc tgatttaata cgtttcatat ctaatccttc ttacaaaacc tctgcggcaa
    20641 gagaaattat cttcataaat ctttctctta actcgcgtgt aaccgggcca aatatacgtg
    20701 ttccaattgg ctcgttttgg ttgtttaaaa gaactgctgc attatcatca aatcgaatca
    20761 atgaaccatc atctcttcga acaccctgag cagttctaac gactaccgct ctcattacag
    20821 cacccttttt taccttgctt cttggtatag catcttttac gcttacttta attacatcac
    20881 ctacacgggc ataccgtctg tgtgatccac caagtacttt aatacacatt actttacgtg
    20941 ctccgctgtt atcagctact tcaagcactg tctgcatttg gatcattttt ctctccagat
    21001 ctaatttcga ccagaaaaag gctgctgatt atatcaggcc ttttagggtt ttaaaagtaa
    21061 aacaattaag ttacttatga aattacttcg actaataccc agcttttcgt tttagagagt
    21121 ggtcgtgact cacggatttt tacggtatta ccaactcggc aaacttgatt ttcatcatgt
    21181 gcgtgtagtt tagttcttcg cttcatgatt ttcccatact ttggatgttt cacagtacgt
    21241 tcaatcataa ctacaatagt tttatccatt ttgtcgctaa ctacttttcc aatcattgtt
    21301 ctggcatttg attcactatt agtagacatt tcacttacct gccttttcag tcaagattgt
    21361 tttaactttt gccactgact tacgcaccat agtgatcaga tgtgttttat ctagtgagcc
    21421 acttgctttt ttcattcgta aattaaattg atctttacgt aatgaaagca attcattttg
    21481 caattcctca actgacatat tgcgcaattc atctattttt ttcattacat taccgtccgc
    21541 tcttcaaaca tcactttgaa aggaagtttt gcttttgcaa ggtcaaatgc ttccattgca
    21601 agctctttgc taacaccttc catttcaaat aaaaccttac ctggttgtat ttgggctacc
    21661 caatactcaa cacttccctt acccttacct tgtcttactt caagtggttt ttgtgtgatt
    21721 ggtttatctg gaaatactct gatccaaatc ttaccaccac gcttaatatg tcgtgtcata
    21781 gcccttcgcg ctgcttcgat ttgtctagca gttaaacgtc cacgctctac agcctttaga
    21841 ccaaactcac cgaaactaat tttactacca cgaagagcca aacctctatt gcgacccttc
    21901 atttgttttc ggtacttagt acgctttggt tgtaacataa tttctaatcc tcactcactg
    21961 ggcgctttca gttgatcttt tcttttgcgg gagaatttca cccttaaaga tccagacttt
    22021 tacgccaata attccgtagg tagtcttgga ttcagcggta ccataatcaa tatcagctct
    22081 aaaagtatgt aaaggcactc gtccctctct gtaccattca cttcttgcaa tttcagcacc
    22141 acccaatcgg ccacttacac aaattttaat accctttgct ccagctttca gagcagaggt
    22201 aacagcacgt ttcatagctc gtctgaacat gactcgttgc tctaactgct gagcaatacc
    22261 ttcagccacc agagtagcat ccaactcagg ctttttcact tcttcaatat tcaaatgaac
    22321 tggcacaccc aggttatcgg aaatttcttt acgaagtgtt tcaattcctc cgcctttttt
    22381 gccgataata acacctggtc ttgctgttaa gatagtcaca acagcgttat tagctggtcg
    22441 ctcaataagt atcttgctaa cagctgcagc catcaatttc tttttaagat cgttacgtaa
    22501 ttttatatcc tgaattaaaa actcagcata tcgctttcct gcaaaccatt ttgagttcca
    22561 atctttaatt attccaaggc gtatacctat tgggtttact ttttgtccca ttgctattcc
    22621 tcgtcagaca ctttaatagt tatatggcag gtacgtttgc aaattcgatt tgctctaccc
    22681 ttagctctgg gactaatacg ttttaaagtt gttgcttcat caacgcatac catgcctact
    22741 ttcagatcat caatatcagc tccattatta ttttcagcat ttgctatagc tgactctaat
    22801 aatttaagca ttaattttgc accttttttt ggtgtaaact tgaggacatc aagcgcacca
    22861 gacacattca tattacgtat catatcggct actaatctac ccttttgtgc ggataaagga
    22921 gcgcctttta atttagctgt tacttccatc atatcctctt acttaccttt agcctttctg
    22981 tccccagaat ggcccttgaa tgtacgagtc atggcaaact cacctaattt atgaccaacc
    23041 atattgtctg taataaatac aggaacgtgg tctttgccgt tatgcacagc aattgttaaa
    23101 tcaatcattt caggaacgat tgtagaacgt ctagaccaag ttttaattgg cttttttgat
    23161 tttgattcga ttgcagcttc taccttgctg atcaaatgat ggtcaataaa tggacctttt
    23221 ctaattgaac gagccacttg atatcctcat attaattatt tcttcttacg tcctctgaca
    23281 ataaatgtgt cagtacgctt attgcttcta gttttataac cttttgttgg caatccccat
    23341 ggagatacag gatgacgtcc acctgatgtt cgtccttcac caccaccatg tggatgatca
    23401 actgggttca tggcgacccc acgaacagtg ggacgtatcc ctctccatcg tttggcacca
    23461 gctttaccca atgctcttag gttgtgttcg ctattactta cttcaccaat tacagctcgg
    23521 cataaaacat gtatcttgcg catctctcct gaacgtaatc ttaaagttgc ataaacacct
    23581 tcttttgcaa caagttggcc actgcaacca gcacttctga gcatttgcgc acctttacca
    23641 ggcttcattt caacacaatg tattgttgta cctacaggta tattttttag tggcagacag
    23701 ttccctacac taattggtga atcagctcca ctttgaatag tagctccaac ttccagattt
    23761 gatggtgcta taatatagcg cttttcacca tctttgtagg atatcagtgc aattagcgca
    23821 gttctatttg gatcatattc caatctctca acacgaccta atataccatc cttattgcgt
    23881 ttaaagtcta taattcgata tttttgacgt tgtccaccac caatatgtct aacagttata
    23941 cgaccttggt tatttcttcc acctgttttt tttagttttt caactaatgc agcatgtggc
    24001 tttcctttat agatatcgtg atgtacaaca cgtatttcac ctcgttttcc gggtgaagtc
    24061 ggtttagatt ttaatagtgc catctttatc tgccttattc agtaactgta aagtcaatat
    24121 cttggtctgc atgaagagag acaaatgcct tcttccagtc acttcgcttc ccactagttt
    24181 gtttgaagcg tttagattta cccttcacat tgacaacaga tacactttta acttttacat
    24241 taaatatatg ttcaactgct aatttaattt cagtcttagt cgcatttttc agtactttga
    24301 aagtgaactg tttaaactta tcggccatga cagttgcctt ttcagaagta tgtggttctc
    24361 taagaaccat catcaatctc tcagcgttca ttgtaactgt tcctcaattt ttttaattgc
    24421 agcttctgta acaacaactt tttcaaatcg tagtaaggaa acaggatcta tagttgtaac
    24481 atcacagata tcataatcaa tcaggtttct tgaaccaagg tattcatttt caccaacttc
    24541 actcattata atcagagcat tacttatttc catctgattc atttttttga ggaaatcctt
    24601 ggtcttgtga ttatcacatt ggaaatcact aacaaccacc aagttgccag tgcggcaaag
    24661 ttcggagatt atactacgta atgcacgttt gtacattttt ttattaagct tttgtgaata
    24721 atctcttttt ttagatgcaa atgttactcc accacttctc cataatggac tgcgaatggt
    24781 tcctgctcgc gcacgacctg ttcctttttg gttccagggt tttttaccac caccactaac
    24841 ttcagaacga gttttttgag cgctattccc gcttcgagca ttgttcatga aagcaactac
    24901 tgcttgatga accaagcctt cgttgtatgt ataagcaaat atctctttat ttagctttaa
    24961 ttttgacttt gtatctattg tagtaatttc catcactcac ctcgtgcttg ctttttgact
    25021 gcaggcttaa tttctactct agaacctgga gcaccaggaa ttgcaccttt aatgagcaat
    25081 aaatttcttt cagagtcaac ttttaccaat tccagacttt gaattgtaca tcgagcatta
    25141 cccatgtgac cggccatttt tttcccttta aaaacacgac caggggtttg gttttgtcct
    25201 atagaaccag gtactctatg tgatcttgag ttaccatgcg atgcatcttg agttctaaag
    25261 ttatgtcgtt ttacagttcc tgcgaaacct ttacctttag tcagacccga tacatcaaca
    25321 tattgtccgg cagtgaatac atcagcaaca gatatgactt gacccggtgt aaatgcatcc
    25381 tctgaatcta tacgaaattc cacttgcata tcaccagcat cgatttgtgc tttagcaaaa
    25441 tgcccagcca ctggcttact cactttgcta gatttctttg taccaccagt taattgtaca
    25501 gctgaataac catcattcgc tgctgttttt acctgagaaa cgcgattcgg ctgaacctca
    25561 acaacagaaa caggaacaga aactccttct ggtgtgaaga cacgcgtcat accgatctta
    25621 cggcctaata aaccgatcat cattgtaaca cctctttaat atcctttgcc tttatattta
    25681 gtcatcaagg cttatctgaa catcaacccc ggcagccaaa tccaatttca ttaaagcatc
    25741 tacagttttt tcagttggat gaacaataac gaccaggcgt ttatgagtac gtaattcata
    25801 ttgatctctt gcatctttat ttacatgcgg tgaagtcaat acagtaaatt tttcttttct
    25861 gataggcaaa ggaattggtc cacgtatttg tgcgccagtt cttttcgcag tatcaacgat
    25921 ttctcgtgtt gatagatcaa tcaaccgatg atcaaaggac tttaatctta ttttaatatt
    25981 ttgattgctg ctcattt
//
LOCUS       DASLOE010000028        24923 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.028, whole genome shotgun sequence.
ACCESSION   DASLOE010000028 DASLOE010000000
VERSION     DASLOE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 24923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24923
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.028"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            31..765
                     /locus_tag="U6309_000938"
     CDS             31..765
                     /locus_tag="U6309_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN5504391.1"
                     /translation="MRKVFSLLLISSIIVISSCSEQKNQNTLRFSTSAEYPPFEYIEN
                     GEIKGFDIDLAKLIAKELGKEAVFDNMQFSAVLPAVNSGQDDVAIATITITEERKKNF
                     DFSKPYYFEGMAAVFDNSQPVKEISQLTGKKIAVQLGSVMEIWLHQNFPKAEITALDN
                     NNQAIEALIAGHVDVVLMDGAQGGVYSKKYPGLSYSILAKANEGYGLVLKKGSPLRDE
                     INSALENLKAKGEIQKLESIWIKGSI"
     gene            762..1409
                     /locus_tag="U6309_000939"
     CDS             762..1409
                     /locus_tag="U6309_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN5504392.1"
                     /translation="MTEIVQNLFFIGKGIVLTMQLLTGGMLIGVLLGTLLSICRHQGV
                     ACFVINRFISILRGTPLILQLSFIYFAVPVLLGMRPSILVAGIVAFGLNSSAYFAEIL
                     RSGIESLPKGQFEAALTLQIPTFYLWKDIILPQVIKNIFPAMINEMIALLKETALIST
                     IGGMDLMRQAQTLAAQQFTYFMPLCITGFYYYSLVLLLEALGKKIEKRGHYAASH"
     gene            1393..2061
                     /locus_tag="U6309_000940"
     CDS             1393..2061
                     /locus_tag="U6309_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN5504393.1"
                     /translation="MLQVINASKKIGDVQVLNEINLSIQANSIVGLVGPSGSGKTTLL
                     RCIQQLESIDSGSIQCEGKSGFMFQDFQLFPHMTVLNNLIYAPSLHDKTADHIANAWA
                     LLAMLGLSDKAHSYPQGLSGGQKQRVALARSLMMKPDLLLCDEPTSGLDMATTNDVIA
                     LLNTVKTLKITMLIASHDLFFLSNIAERLLVVRQGQLIADFKTQEVSDPILYLQQFYQ
                     GEIL"
     gene            2058..3275
                     /locus_tag="U6309_000941"
     CDS             2058..3275
                     /locus_tag="U6309_000941"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEN5504394.1"
                     /translation="MKKVIKKIALAYSGGLDTSIMIPWLKEHYEHAEVIAVICDLGQQ
                     EDLDAIKNKALKSGASKAYVVDVKNEFAIQYLWPLVKSGALYEDQYILGTISRPLIAQ
                     KLVEIALTEQVNAVAHGATGKGNDQVRFEYSIKALAPQLEIIAPWRTWDIKSRQEAIV
                     YAKAHGIEVPVTPKAPYSRDHNIWYISHEGGVLEDPSQEMPDDVLLMTAPVSQAPDEE
                     EVVVLDFKKGVPVALNGQELSPVDLLNSLNQKAGQHGIGVADIVENRLVGMKIRGIYE
                     APAAAVLYKAHKLLESLCLTRSTLHLKQSLQQTYANLVYEGRWFSQTKQALDAFIDVT
                     QQHVTGCVKLKLFKGNIIPAGMHSPYSLHHPELATFEEDNVYNQKDAEGFINLFSLSA
                     KIYSQVHQGGNYD"
     gene            3268..4503
                     /gene="argH"
                     /locus_tag="U6309_000942"
     CDS             3268..4503
                     /gene="argH"
                     /locus_tag="U6309_000942"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEN5504395.1"
                     /translation="MTNKTWGGRFKKSLDSGVNQFNASLSFDHVLFEQDINGSQVHVK
                     QLAKQKILTEVESQAIYSALEEIRSEIKQGQYSFTERDEEDIHMFIEQLLIQKIGDLG
                     KKLHTGRSRNDQVALDLRLYTRDKGCLINELLTRLIDCLDDLTSKHQQDLMPGYTHLQ
                     QAQPVTLGAYFNAYQCMFSRDKSRLEDWFKRMNYSPLGAGALAGSTLPLDREWVAESL
                     GFAGIIPNTLDAVSDRDFVIELCSVAAMIMMHLSRLCEDLILWSTQEFNFVILDDAFA
                     TGSSLMPNKKNPDVPELIRGKSGRVYGHLMAILTVMKGLPLAYNKDMQEDKEGLFDTI
                     NTIIACLQMITPFLQSLTFNTLLMKTKAQSGYLDATAILESLVMKGMPFRDAHHQVGA
                     WIAEAIEKQCSLNELLKGG"
     gene            4506..5621
                     /gene="argF"
                     /locus_tag="U6309_000943"
     CDS             4506..5621
                     /gene="argF"
                     /locus_tag="U6309_000943"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEN5504396.1"
                     /translation="MSFSTSTNLTPTEMQDDDLEKIQSQNNPSAINEVKTNTFVINLQ
                     QAIFGLETGSEAVDFDSHKNTGFPFPAGEYKPRHFLTGTELAREEIQFVLSQARSLKQ
                     NPGLYNQALANKSLVMIFEKPSFRTRLSFALAIQSMGGIVVESIANSRKQETPADMAR
                     VLNGYADFVMVRTHEDSFLQEMAEYSKIPIINGLSALHHPCQILADLLSLEEQFGALE
                     GLTLTYIGDGNNILHSLMLLAPQLGVTVHYCCPKSNQPNEAIVKEAQSKTGKQMIYCF
                     DKPEEAVKQADAVYTDVWTSMGFDNPEAGKIFKGFQVNETLMSHAKPDSVFMHCMPME
                     RGKEVSATLPDSASSIIFRQSENRMHVQKALMLFLDQ"
     gene            complement(5710..7185)
                     /locus_tag="U6309_000944"
     CDS             complement(5710..7185)
                     /locus_tag="U6309_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEN5504397.1"
                     /translation="MNITRLLAFFTSFFLLCPIYASVDSYFSHIKKDSSALYAFFKTM
                     PKGGELHYHLAGGAYPETMLALAANGNYCLDKHSFAVSKDSSHCEGVNVKEVFNNPEL
                     YSNIIKDWSMKDFIPGKESGHDHFFNGFVKYMPIVFDYRPQLLADIVQRAAQQKEQYM
                     EIMIIPDNAHSLTYGDLIKDTPSYDQKRRLLLANKDFQSNIKNTVLESDRILTKASQE
                     LGCKDNPSSDFCLVKIKFIYYVLREQPVDNVFAQALNAFEAVSKSKGNLIGVNLVQPE
                     DGIISLRDYHKHMSIFKYLHRIYPNVPITLHAGELSPQAVTPENLSNHIRDALLIGHA
                     QRIGHGVDIGYENNAEDTLKYMANHHIPVEINLISNLKILNISGRNHPLNYYLTHNVP
                     VVLSTDDEGVLRTDLTQQYVEAAKEHGLNYQQLKQINRNTLTYSFLPGKSIWANADKA
                     ELIPDCHDLGSKSCQKFIEHNEKAKLQWSLEQQLIAFEKNY"
     gene            7360..8475
                     /locus_tag="U6309_000945"
     CDS             7360..8475
                     /locus_tag="U6309_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN5504398.1"
                     /translation="MPYFYTRSAPLFFVFVIALIFINFSYAHEFQTDSKNKHKVDVHI
                     GIYAPFSSHSAFIGRNILAAMEMGSERLQSSDIHYSFYTLDQLPKNKNAGLVLQKFID
                     THHINVLVTGGKETGLIAAPIAKKNNLIHFSLTNNTKVADGRNNFIVWSPVYDQAKVL
                     VNELKRQQVHHLGIITANQPSSTALTHSVIKQLQADSHIDVAASEFYEPGNKDFSRLA
                     NRIKRKNADMYLVMASPEDIKLIQEQMEADKQYVPITSIVERATPEVVKVFEGQWYVD
                     SHEMQPDFINEFKQNYLNYPVTEAGYAFDLFQLLNKSIVMAIRTQGSPTSQEVVHQLH
                     DIANRTGVMGTMVLDSKGSLFTKSEVKTVKNGEIHTV"
     gene            complement(8547..9884)
                     /locus_tag="U6309_000946"
     CDS             complement(8547..9884)
                     /locus_tag="U6309_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEN5504399.1"
                     /translation="MVIKKLFSSTLALVYALTLMLPLTAFSAEETNSNYSNTKRIPLE
                     DVQRFSNAIGEIKKYYVKPVDDKELFDNAIRGMLTGLDPHSSYLNEEEFKELQTSTSG
                     EFGGLGIEVTMEEGVVKVITPLVDTPAFKAGIKSGDYIIKLGKESVQGLSLKDAVNLM
                     RGKPGTTIELTILRKGVNKPLTFDLIREVIQIKSVKSKMLSEGYGYIRLTQFQALTGK
                     DMIKAIEQLKQQAGGKLKGLVLDLRNNPGGLLDSAIQVSDAFLGNDKAGKQEMIVYTE
                     GRLPGSKFTALANPGDVLDNAPIVVLINNGSASASEIVAGALKDNKRAIILGTKSFGK
                     GSVQTVLPLDGKTGIKLTTALYYTPSGVSIQAKGIIPDIIVEEVDVPKNAVKKDTLAG
                     FTEADLNGHLINKDNSENTESKNNQIQTGDLIHEDYQLYAALTVLEGMALANR"
     gene            complement(9965..11107)
                     /locus_tag="U6309_000947"
     CDS             complement(9965..11107)
                     /locus_tag="U6309_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN5504400.1"
                     /translation="MRMPSLPNYLSSAPLGLALMVILFFCNGLEAKAAPNSITQAQTK
                     LKQLDAKINKLKQVLANAQDKRTVLNQELGSTEKQIGEGIRKLRIIQANMSAKEKSIA
                     ELQGKINHLNKRLQAQQEMLANHVRARYQMGEYQPLKWLINQDEPFKINRILTYYQYI
                     IRSRQQLIDEIARTRQNLNDSKTKLGNELAENKQLQFKLSQNQKQLEQNKRYHSTLIQ
                     SIDNDIQNNQQSLKDFQKDRDNLSHLMKSLSQQSVIQSGKPFTGMRKKLPLPVQTAHR
                     SLRRMNQGVTFFADEGSIVTAVYPGKVVFSDWLKGYGLLLIIDHGQGFMTLYAHNQSL
                     FKRKGQIVQQNEQIASVGHTGGIKQNGLYFEIRQRGKAVNPLDWLS"
     gene            complement(11094..12638)
                     /locus_tag="U6309_000948"
     CDS             complement(11094..12638)
                     /locus_tag="U6309_000948"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEN5504401.1"
                     /translation="MSHNAPLVLMILDGWGYNENDRYNAIAKANTPQWDEWWQTCPHI
                     LLKASGLPVGLPDEQMGNSEVGHMHIGAGRVIQQDFTRINEAINNGKFAKNAVFHEVI
                     DQLKKTEKSLHIMGLLSPGGVHSHEQHLFALLTLCNQKKFRSVHLHLFLDGRDTPPQS
                     ALDSLKCLNEELAKHPVATINSICGRYYAMDRDKRWERVEPVYNLLTQGKSERQFPDA
                     ETAIHFYYKNKISDEFVPPTLIGKEHSIQDGDAVLFFNFRADRARQLTSTFLDPLFKG
                     FERKTLPKLSYFVSMTQYDKNLFTTTAFPPVPLNNTLGEVLSSHGLSQLRIAETEKYA
                     HVTFFFNGGCESVFTNEERIMVPSPQVATYDLQPEMSAHELTKTLIAAINSRDYHVII
                     CNYANADMVGHTGNFEATVQAIECLDQCMQQVWQALKNNGGKLLITADHGNAEEMFSE
                     ATNQAHTAHTSEPVPFLYVGGGWHFTHSEGSLIDIAPSLLALLGITPPPEMTGRILLE
                     KNHAHA"
     gene            complement(12741..13037)
                     /locus_tag="U6309_000949"
     CDS             complement(12741..13037)
                     /locus_tag="U6309_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504402.1"
                     /translation="MSRDTRNLILKAIILFISLTALVNHAMAFNLLGIGPNPKNLPKE
                     NDVRASFNHCKSCDKSTTPPLIITTPVPIIYYPGCRPIQSCDAYGHCEQKEDCY"
     gene            complement(13114..14385)
                     /locus_tag="U6309_000950"
     CDS             complement(13114..14385)
                     /locus_tag="U6309_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEN5504403.1"
                     /translation="MKKSIHRFVKPRLFAMSISLLVFSSITYGESNGNFALSEHWSMG
                     QQIKLRFDINEVPQSGVVLHLKNGLALTAGDIISLGDLYGIVGKPISHGLTKLEKQTR
                     FTEVFNSFARNVIAVSEVEDLNSVIRAEMREVESGIAKGETAEAIYKRIGNEVGRQIN
                     CITGGGCNSLTWWLNPGRYLKLAMENYDHFTPNNLIAYKSGHHVALQQALKAKRTGKR
                     SDLEIAYAMDAFACHYLSDHFAAGHLRTPREELSAIITPTVLGALLSNYMHNEENKYG
                     IHVTNNLGEQWMVYGDYSYFNPFNQTNQQMLLKALQASADEIFEAYYSGVLPEKNQIE
                     DMIPHITKLNDENNMDIAPMFYWDKENKQLLRRTNLTNPYDRHWTNSWWGWSTLLLLK
                     TQYGITSTVQLSLSKYLSQYVPEELQDNPLV"
     gene            14735..15451
                     /locus_tag="U6309_000951"
     CDS             14735..15451
                     /locus_tag="U6309_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEN5504404.1"
                     /translation="MNHKLPQHIAVVMDGNGRWAESRGLPRVEGHKAGLDSVKKIINC
                     CLEKKISCLSLFAFSSENWSRPVTEVNFLMELFLEALRKEIDDLNQHGIRLKFTGDRE
                     PLSQVLQKQMCDAEALTKNNQQLILNVVVNYGGKWDIVTAARKLIRTVLDGKLAYDEI
                     NEAVLAQFLDTNGMPEPDLFIRTSGELRISNFFLWQLAYTELYFTDVHWPDFNEHEFE
                     LALTSFAKRARRFGQISQSE"
     gene            15462..16259
                     /locus_tag="U6309_000952"
     CDS             15462..16259
                     /locus_tag="U6309_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEN5504405.1"
                     /translation="MFMQRLITTLILAPLVLLAIFYAPSWILWGVVLVILLLAGMECF
                     KLIPVDNLTVQACFLIFLLMCFWACGYFYPYWLSVGLLVWLLNGLAILTYPRSQNYWG
                     YPIVVAIASLILLPLFTQSLIQISFLQQGKELLVYLLFLIWASDIGAYLTGKQWGKHK
                     LIPKVSPGKSWEGVTGGVILAMIVATLGYYYFIPYSIYIWFGLALSTVVISIFGDLFI
                     SILKRRCQLKDTGNIIPGHGGILDRLDSLIAALPWFYFGLTYIPLGI"
     gene            16292..17644
                     /gene="rseP"
                     /locus_tag="U6309_000953"
     CDS             16292..17644
                     /gene="rseP"
                     /locus_tag="U6309_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEN5504406.1"
                     /translation="MLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVLRFSFGFGKI
                     LARFYDKKGTEYAWSLFPLGGYVKMLDETEGEVSEKEKPFAFNNQSVFVRIAIVVAGP
                     LFNFIFAFVALWLVLVIGMHSLAPMIESVKPNSIAARAGLVPKQEILALNGVKINSWR
                     DFQYEIMPLIGSQETVSLTVKSMINGEESTLYLPLDKWQLDDKKPDPIKSLGIVPFIP
                     TIPPIVGEVVPDSPAEKAGLKIGDEIISVNGQQFNDWLYLVSYVRERPNSQINLDIKR
                     QGKLLKITVHTGSQDNNGKLEGLIGVRSQKVDWPAHWLRLEQQPPISALGTAFKQTVQ
                     LTGTTFILMGRLVTGKLGLNSISGPVGIAQGAGDSGRGGLVSYLFFLALVSISLGALN
                     LLPIPMLDGGHLLYYVLEIIRRKPLSDGVKSVGIYFGLLLLVALMFVALSNDISRLTS
                     "
     gene            17815..20127
                     /gene="bamA"
                     /locus_tag="U6309_000954"
     CDS             17815..20127
                     /gene="bamA"
                     /locus_tag="U6309_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEN5504407.1"
                     /translation="MKKVSNKLILGVCCSTLLAWSSQTFSSDTFIVKGIRVNGLQRVS
                     TGTVLNYMPVQVGEEISSSSTAQIIRALYETGFFQSVSLERQGNVLVVNVVERATIGS
                     ITVVGNKEIPSDKMKAFLKEMGLVKGRVFQRSSLERLEKELKQAYTARGKYNSRIETK
                     VTPLTENRVAISITVSEGRVSRIKEIKIIGNHDFKANELLPELTLSTSNLSTYFTKKD
                     QYSKAGMDASLEALRSFYLDRGYLKFNVVSSQVLLSPDKKDVYINIHIEEGPQYHFSG
                     YDVVGKTILPKEKIDSLIQVKKGDVFSRKKVTESISAIGLALGDVGYGFPAINAEPRI
                     DENNKTVFITFVVQPGRHVYVRRINFHGNTKTGDYVLRNVIRQDEGGLLSLHNIKESE
                     RQLRMLGYLKNIDVKTTPVPGTNNQVDLDVNVEEAPSAEASASMGYGTNGYQFNASVN
                     QRNFMGSGRSMGAAFNASQWGQDYSFNYYNPFYTDTGVGRGGSLYYSRIDPKNLNVST
                     YSSNRYGGDISYNFPLGEKSSFQLGYGYQDINIKSVGYVLPIINFVALNGNHFQEIRL
                     TSGWSRNSYDQMPYPNQGFNQQAIAMVALPATSQSLSYYKSSYQAHLYYPLTRGWIFS
                     VLGNVGYGNTFDNFGLPFFENYYAGGPVQPGQVRGYDSYSLGPQDNFGNAMGANFLVN
                     GSVGLILPYPLSRDNVRTTIFADAGNVFASGTPAALRGTPAGPMRYSAGVSLEWRSPF
                     GPLSFSLAKALNPQPLDQTQLFQFALSSGF"
     gene            20241..20741
                     /locus_tag="U6309_000955"
     CDS             20241..20741
                     /locus_tag="U6309_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEN5504408.1"
                     /translation="MKRLGAVLVALFLGLFGTTVFADWAKIGVVDLQKIMQTSNQMKE
                     IQQKLEKEFKPRRDKLVAMEASLKSDMEKFKRDSAIMSASQKKELEKKIVASQQQFER
                     DGQQYQQELSTAHNEAMEGLYNKVRTAITKIAKDEKYDIIVQKDAAPFSSESLDVTDK
                     VIKAIN"
     gene            20755..21774
                     /gene="lpxD"
                     /locus_tag="U6309_000956"
     CDS             20755..21774
                     /gene="lpxD"
                     /locus_tag="U6309_000956"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEN5504409.1"
                     /translation="MLTLEELANYLNGVWYGNANHAIFSLSSLARASSKDLAYFDNPL
                     LQSVLNSTRAGAVLLKPEHKDWCLVNCIVVPNPSEAMFKAAKILPHLATNVCGIHPTA
                     QIHKSAQIGQHVSVGANSVIGENVQLDDYVTIGSNTTIESSVLIGRGSQLGSGSIIHS
                     GTVLGQSVIIDSGCIVGAAPFNCYKEHGVWQQAPNFGGVVIGQRTQIGANTVIHRGSI
                     GDTYLGDGVCIDSLVLIAHDVYIGNNTAIAGSAAIGALVQIGCDCIIGGASCLAANIR
                     LTNDVVITGMSTVTKSIARPGVYSSGTTVHDHRKWRRNTARFRHLDDYITKLITIEKK
                     LNNNN"
     gene            21892..22344
                     /gene="fabZ"
                     /locus_tag="U6309_000957"
     CDS             21892..22344
                     /gene="fabZ"
                     /locus_tag="U6309_000957"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN5504410.1"
                     /translation="MNESIDINQIFTLLPHRYPFILVDRVIDYKVMEYLIAIKNVTIN
                     ENFFTGHFPGNPIMPGVLMLEALAQACGILANLSRQPKEGYEFLHYFAGIDNARFKQV
                     VIPGDQLRLEVRLAGQKRDFWRMHGEAYIGDKLACSADLLSAAKEIKK"
     gene            22341..23111
                     /gene="lpxA"
                     /locus_tag="U6309_000958"
     CDS             22341..23111
                     /gene="lpxA"
                     /locus_tag="U6309_000958"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEN5504411.1"
                     /translation="MIDERAMIHPSAKLASGVSVGPGTVIGADVEIGENTWIGPHVVI
                     EGPTVIGKNNKIFQFASVGDEPQDITYKGEPTRLEIGDNNVIREYCMISRGTVKGGGV
                     TRIGNSNYLMAYSHIGHDCMVGNHIIMVNYAALSGHVTINDYAIIGPYAAVHQFCQVG
                     AYAFIARATYVTKDVLPYVMIAGHTTSACGINTVGLRRRGFSSAAIDCLRRAYKIIFR
                     KGLTVQQAVSELELIQNECPEIIPMIDALNQSTRGIVR"
     gene            23115..23519
                     /gene="crcB"
                     /locus_tag="U6309_000959"
     CDS             23115..23519
                     /gene="crcB"
                     /locus_tag="U6309_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEN5504412.1"
                     /translation="MVVAPYLAVAIGGSLGAMSRYLVTIMAQNAWGIKFPYGTLLVNT
                     LGSFLAGFFLIVLVGRFSAEESFRLFLFTGFLGAFTTFSSFAAESLFMFEQGYWFKLI
                     TNILVNNVGSLSMVFIGTLVAKYVLLGHQGSN"
     gene            23541..24821
                     /gene="serS"
                     /locus_tag="U6309_000960"
     CDS             23541..24821
                     /gene="serS"
                     /locus_tag="U6309_000960"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEN5504413.1"
                     /translation="MLDNQLLRENPQYVATQLLKRGFQFDAVTFSQLEEKRKALQVST
                     QSLQNERNLRSKAIGEAKSRGENIEPMREEVNKLGAKLEQQKTELDEILKQIEVISLS
                     LPNIPHESVPVGKDELDNQEIRKWGDVPAFSFPVKSHDELGEALGQMDFALAAKITGS
                     RFVVMKGHLARLHRALIQFMLDIHIQQHGYQEIYVPYIVNADSLLGTGQLPKFEADLF
                     KLTGDNGYYLTSTSEIPVTNTVREMILSAEQLPIRYVCHSPCFRSEAGSYGKDTKGMI
                     RQHQFEKVELVWITKPEDSYNALEQLTQHAEVILQRLNLPYRVVALCTGDIGAGSAKT
                     YDLEVWLPSQNTYREISSCSNMEAFQARRMKARFRNPDTNEIQLVHTLNGSGLAVGRT
                     LVAIMENYQDEHGNIHIPDALKPYLGGIDIISVK"
BASE COUNT         7325 a         4371 c         5277 g         7950 t
ORIGIN      
        1 aaaaatgcat aaatacggga ttcatattca atgagaaaag tttttagttt attgttaatt
       61 agttcaatta tagttatatc cagttgtagt gagcaaaaaa atcaaaatac actgcgtttt
      121 tccacttcag cagaatatcc accttttgaa tatattgaaa acggagaaat aaaaggattt
      181 gacattgatt tggcaaaatt gatcgccaaa gagctaggta aagaagcagt ttttgacaac
      241 atgcagttta gtgcggtttt gcctgctgtg aattcaggcc aggatgacgt tgcgattgcc
      301 acaattacta ttaccgagga acgtaagaaa aattttgatt tttccaaacc ttattatttt
      361 gaaggaatgg ctgcagtttt tgataattca cagccagtga aagagatctc gcaattaaca
      421 ggaaaaaaaa tagcagtcca gctgggtagt gttatggaaa tttggcttca tcaaaacttt
      481 cctaaagctg aaattacggc tttggataat aacaaccaag cgatagaagc tttaatcgca
      541 ggccatgttg atgtggtcct tatggatggg gcacaagggg gtgtttatag caagaaatat
      601 ccaggactgt cttattctat cctggcgaaa gctaatgaag gttatggatt ggtcttaaaa
      661 aaaggttctc ctctaaggga tgaaatcaat tccgcattgg aaaatcttaa agcgaagggt
      721 gaaattcaaa aattggaatc tatttggata aaggggtcaa tatgacggag atagtgcaaa
      781 atctgttttt cattggcaaa ggaatagttc taactatgca attattaaca ggaggtatgc
      841 tgattggagt attacttgga actctcttgt caatttgcag acatcaagga gtagcttgct
      901 ttgttataaa ccgattcata tccatattga gaggcacacc actcatttta caattaagtt
      961 ttatttattt cgcagtgcct gttttattgg gtatgcgacc aagtatttta gtggctggaa
     1021 ttgttgcttt tggtttgaat agctcagctt atttcgccga aattttaaga tctggaattg
     1081 agagtttacc caaagggcaa tttgaagcag cccttactct gcaaattcct accttttact
     1141 tatggaaaga tattatttta cctcaagtga taaaaaatat ttttcccgca atgatcaatg
     1201 aaatgatcgc cttgttaaaa gaaacggcct tgatttccac tattggaggc atggatttaa
     1261 tgcgtcaggc tcaaactttg gcagcacaac aatttacgta ctttatgcct ttatgtatca
     1321 cgggtttcta ttattacagt ttggtcttac tccttgaagc actaggtaaa aaaattgaaa
     1381 aaagggggca ttatgctgca agtcattaac gcatcgaaaa aaatcggcga tgtccaagta
     1441 cttaatgaga ttaatttaag tattcaagca aacagtattg ttggtttagt agggccttct
     1501 ggtagtggaa aaacaacttt actgagatgc atacaacaat tggagtcaat cgattcaggc
     1561 tctatccagt gtgaaggaaa aagcggattt atgtttcagg attttcaatt atttccgcac
     1621 atgacagtac tcaacaacct aatttatgca ccaagtcttc acgataaaac tgctgatcat
     1681 atagctaacg cgtgggcttt acttgctatg ttaggtttgt cagacaaagc ccattcttac
     1741 ccacaaggat tgtctggtgg acaaaaacaa cgcgtggcct tggctagaag tttaatgatg
     1801 aaaccggatt tattgctttg tgatgaaccg acttctggtc tagatatggc gaccactaat
     1861 gatgtcattg ctctattaaa tacagtgaag actctaaaaa tcactatgct tatcgcttct
     1921 catgatttgt tttttttatc caatattgcg gagcgattgt tggttgtaag gcaagggcaa
     1981 ctaattgctg actttaaaac ccaagaggtg tctgatccta ttttatatct acagcagttt
     2041 taccaggggg aaatattatg aaaaaggtta ttaaaaaaat cgcattagct tattcaggtg
     2101 gcttggatac ctccatcatg atcccttggc ttaaagaaca ttatgagcat gctgaagtaa
     2161 tcgctgtgat ttgtgattta gggcaacagg aagatctcga tgccataaaa aataaagcgc
     2221 taaaaagcgg cgcttccaaa gcgtatgtag tcgatgtgaa gaatgagttt gccattcaat
     2281 atctttggcc tttggtaaaa tcaggtgctt tatatgaaga tcaatacatt ttgggcacta
     2341 tttcaaggcc actaattgct caaaagcttg ttgaaattgc tttgacggag caagtaaatg
     2401 ccgttgcaca tggagccacc ggtaaaggga atgatcaagt gcgttttgaa tattcgatta
     2461 aggcacttgc acctcagctt gaaattattg ctccctggag aacttgggat attaaatcaa
     2521 gacaggaagc aatagtgtat gctaaagcgc atggcattga ggtaccagtg actcccaaag
     2581 cgccttattc tagagatcat aacatttggt atatttctca tgaaggtggt gtgctcgaag
     2641 atccttctca ggaaatgccg gatgacgtgt tattaatgac tgctcctgta tcacaggctc
     2701 ctgatgaaga ggaagtggtt gttcttgatt tcaaaaaggg ggttccagtc gccttaaatg
     2761 gacaagaact ttctccagtg gatctcttaa attcattaaa tcaaaaagca gggcagcatg
     2821 gtattggagt ggcggacatt gttgaaaatc gactagttgg aatgaaaatt cgagggattt
     2881 atgaagcacc tgctgctgcc gtgctttata aggcacataa acttttggag agtttatgtc
     2941 tcactcgttc tacgttacat ctgaagcaat ctttgcagca aacttatgcc aatttggttt
     3001 atgaaggaag atggttttct caaaccaaac aggctttgga tgcattcatt gatgtgacgc
     3061 agcagcatgt cacaggctgt gttaagttaa agttattcaa aggaaacatt atccctgccg
     3121 gtatgcattc tccctatagc ttgcatcatc ccgaattggc tacttttgaa gaggataatg
     3181 tatacaacca aaaggacgct gaaggattta tcaacctctt ttctttatcc gctaaaatct
     3241 atagccaggt acatcagggg gggaattatg actaataaga catggggagg gcgatttaag
     3301 aaatctttgg atagcggtgt caaccagttt aatgcgtcat tgtcttttga tcatgtgctt
     3361 tttgagcagg acattaatgg cagtcaggtt catgtgaagc aattagctaa acagaagatt
     3421 ttaacagaag ttgaaagcca ggcgatttat tctgctcttg aagaaatcag aagcgagata
     3481 aaacaaggcc aatattcatt tactgaacgt gatgaagaag acatacatat gtttattgag
     3541 caattgctga ttcaaaaaat aggcgatttg ggtaagaaat tacatactgg ccgtagtcgt
     3601 aatgatcaag tggctttgga tcttcgttta tataccagag ataaaggctg tctgattaat
     3661 gaacttttga caaggcttat cgattgtcta gatgatctaa ccagtaaaca tcaacaggat
     3721 ttaatgccag gctatactca tttacagcaa gcccaacctg ttacgttagg ggcttatttt
     3781 aacgcgtatc aatgcatgtt tagccgagat aaaagcagat tggaagattg gtttaaacgc
     3841 atgaattatt ctcctttggg cgccggggca ctggctggaa gtaccttgcc tcttgataga
     3901 gaatgggtag ccgaatcctt gggatttgca gggatcattc ctaatactct tgatgcagtc
     3961 agtgacagag attttgtcat tgagttatgc agtgttgctg caatgattat gatgcatctc
     4021 tccagattat gtgaagattt gattctatgg tctacccagg aatttaattt tgttatattg
     4081 gatgatgcgt ttgctacagg ctcttcactg atgcctaaca agaaaaatcc cgatgttcct
     4141 gaattgattc gtggcaaatc aggacgagtt tatggtcatc taatggccat tttaacggta
     4201 atgaaagggt tgcccttggc ttataacaaa gacatgcagg aggataaaga aggattattt
     4261 gatacaatta atacgataat cgcttgcctg caaatgataa ctcctttctt gcaaagtctg
     4321 acttttaata ccctgttaat gaaaaccaaa gctcaaagtg gttaccttga tgcgactgcc
     4381 attctggagt cattagtgat gaagggtatg ccttttcgtg atgcacacca tcaagttggg
     4441 gcatggattg cagaagcgat agaaaaacaa tgttcactga atgaattatt aaaaggaggc
     4501 taagaatgtc tttttccacc tcaaccaatt tgactccaac tgaaatgcaa gacgatgacc
     4561 ttgaaaaaat tcaatctcag aataacccat ctgcaattaa tgaagtgaag accaatactt
     4621 ttgtcattaa tttacaacaa gctatttttg gcctggagac gggtagtgag gccgttgatt
     4681 ttgattctca taagaataca ggctttccat ttcctgccgg tgaatacaaa ccaagacatt
     4741 ttcttactgg cacagagtta gcaagggagg aaattcagtt tgtcttaagc caggctcgct
     4801 ctctaaaaca aaacccggga ttatataatc aggcacttgc caataaaagt ttggtaatga
     4861 tttttgaaaa accttccttt agaacccgtt tgagttttgc tttagcgatt cagagtatgg
     4921 gggggatcgt tgttgaaagc atcgctaact caagaaagca ggagactcca gctgatatgg
     4981 ctagagtttt aaatggctat gctgattttg taatggtcag aacacatgag gacagttttt
     5041 tgcaagaaat ggccgaatat tccaaaatcc ctattattaa tggcctctcc gcattgcatc
     5101 acccttgtca aattcttgct gacttgttaa gccttgaaga acaatttggt gctttggaag
     5161 gattaaccct cacttacata ggggatggca ataatatttt gcatagtttg atgcttttgg
     5221 caccgcagtt aggtgtcact gtgcattatt gttgcccaaa atcaaatcag cctaatgagg
     5281 ctattgttaa ggaagcccaa agcaagacag ggaaacaaat gatttattgt tttgataaac
     5341 cagaggaagc tgtgaagcaa gcggatgccg tttataccga tgtgtggaca agtatgggat
     5401 ttgataatcc tgaagcgggc aagattttta aagggtttca ggttaatgag acattaatgt
     5461 ctcatgcaaa acccgattcg gtgtttatgc attgtatgcc tatggaaagg ggaaaagagg
     5521 tttctgctac tttacccgac agcgcgtcat ctatcatttt taggcagagt gagaatagaa
     5581 tgcatgtaca aaaggccttg atgttatttt tggatcaata gaaccttgaa ttatgaggcc
     5641 catatttgtg cataataaac gctattgcgc attccttctg atatgggctc attatttttg
     5701 caaattaatt tagtaatttt tctcaaaggc gattaattgt tgttccagac tccattgcaa
     5761 ttttgctttt tcgttgtgct cgataaattt ttgacagctt ttgcttccaa ggtcatggca
     5821 atcaggaatc aattcagctt tatccgcatt agcccatatg ctttttccag gtaagaagga
     5881 gtaagttaat gtatttctat taatttgttt taattgctga taattaaggc catgctcttt
     5941 ggctgcctct acatattgtt gggttaagtc cgttcttagc actccttcgt catcagtgga
     6001 taaaacgact ggtacattat gagtaagata ataattaaga ggatgattgc ggccagatat
     6061 attcagaatt tttaaattac taatcaaatt gatttcaacg ggtatgtgat ggttagccat
     6121 gtattttaag gtatcttctg cattgttttc ataaccgata tccacaccgt ggccaattct
     6181 ttgggcgtgc ccaatcagca gtgcatcgcg aatatgatta cttagatttt ctggtgtcac
     6241 tgcctgaggt gataattctc cggcatgcaa ggtaatagga acattcgggt atattcgatg
     6301 cagatattta aagatggaca tgtgcttatg gtaatcacgc aaagaaataa ttccatcttc
     6361 cggttgcacg aggtttacgc cgattaagtt ccccttagac ttggagacgg cttcaaaggc
     6421 attcaaggct tgggcaaaga cattatcaac gggctgctct ctgagtacat aataaataaa
     6481 tttaattttt acaaggcaga aatcggaaga agggttatct ttgcagccta attcttgaga
     6541 tgctttagta agaattcggt cactttccaa aacagtattt tttatgttgc tttgaaaatc
     6601 tttattcgct aatagcaggc gccttttctg gtcatatgat ggagtgtcct tgattaaatc
     6661 cccataagtt aatgagtggg cattgtccgg gattatcata atttccatgt attgctcttt
     6721 ttgctgtgct gctcgctgaa caatatcggc aagcaattga ggcctataat caaaaactat
     6781 gggcatatat ttgacaaatc cattgaaaaa atgatcgtgg ccagattctt taccaggtat
     6841 aaagtctttc atagaccagt ctttaattat gttggaatag agttcaggat tgttaaaaac
     6901 ctccttaaca ttaactccct cacaatgaga agagtcttta cttacggcaa atgagtgttt
     6961 atcgaggcaa tagttgccat ttgcggcaag tgccagcatg gtctcaggat aggctcctcc
     7021 agccaaatga taatgtaact cgccaccctt gggcattgtt ttaaaaaagg cataaagggc
     7081 actggaatcc ttttttatat gactaaaata ggaatcaaca ctggcataaa ttgggcacaa
     7141 aaggaagaaa ctggtaaaaa atgcgagtaa tctggtaatg ttcataattt caatgtgatc
     7201 attaattggg cagatattat ctcataatct tgcacgattc ccaacagaat atctgtttta
     7261 tcatgcaatc tcttatgatt ttttacgaaa tgataatgat tgtatttttt tattattcat
     7321 tgcagaatgt ttttaaggtt taacacatgg aagaaaaaaa tgccctattt ctatacaaga
     7381 tcagcgcctt tgttttttgt attcgttatc gcacttattt ttataaattt ttcctatgcc
     7441 catgaatttc aaactgattc aaaaaataaa cataaagtgg atgttcacat aggtatctat
     7501 gctccgtttt ccagtcattc agcttttatt ggaagaaata ttctggctgc aatggaaatg
     7561 ggcagtgaac gcttacagtc ctctgatatt cattattcat tttatactct ggatcaactt
     7621 cctaaaaata agaatgctgg tcttgtgctg caaaaattta ttgataccca tcatatcaat
     7681 gtgttggtga ctggaggtaa agaaacgggg ttaatagcag ctcccatagc aaagaaaaac
     7741 aatttgattc attttagtct gactaacaat actaaagttg cagacggcag aaataatttt
     7801 attgtctgga gtccagttta tgaccaagcc aaggttttgg ttaacgagct aaaacgtcag
     7861 caagtccatc acttgggaat tattacggca aatcaacctt cctcaaccgc tttgacgcat
     7921 agtgttataa aacaattaca ggctgattct catattgatg tggctgcctc cgagttttat
     7981 gaacctggaa ataaggattt ttcaagatta gctaatagaa ttaaaagaaa aaatgcagat
     8041 atgtatttgg taatggcatc accggaggat attaaattaa tacaagaaca aatggaggca
     8101 gataaacagt atgtacctat taccagtata gttgagcgtg ctactccaga agtggtaaag
     8161 gtatttgaag gtcaatggta tgttgattct catgagatgc agcctgattt tattaatgaa
     8221 tttaaacaaa attatttaaa ttatcctgta actgaggcag gttacgcgtt tgatttattt
     8281 cagcttttga acaaaagcat agtaatggcg ataagaacac aaggttctcc tacaagccag
     8341 gaagtggttc atcaactcca tgacattgcc aatagaacag gtgtaatggg aactatggta
     8401 ttggacagta agggttcttt atttaccaaa tctgaagtga aaactgtgaa aaatggtgag
     8461 attcatactg tctaggtaga aaataaaatc tgaaatcatc agttcatagt gaatcgatga
     8521 tttcaggcgg gaggactaat tggtcattat ctgttagcta acgccattcc ttccaatacg
     8581 gtgagtgccg catataactg ataatcctcg tgtattaaat ctccagtttg aatttggttg
     8641 tttttggatt cagtgttttc tgaattgtct ttgttaatca agtgcccatt aaggtccgct
     8701 tcagtaaaac cagccagagt gtcttttttg actgcatttt taggaacgtc tacttcttct
     8761 acaatgatgt caggaattat tcctttggct tgaatggaga ctcctgatgg ggtgtaataa
     8821 agtgctgtgg ttagttttat tccagttttg ccatcaagag gtaatacggt ttgaacggat
     8881 ccttttccaa aactctttgt accaaggatt atagctcttt tattatcttt taatgctcca
     8941 gcgacaattt ctgaagcaga ggcagaacca ttattaatta agacaacgat aggcgcattg
     9001 tctaacacat cccctggatt ggccagggcg gtgaatttag agccaggtag tctgccttcc
     9061 gtatagacta tcatttcttg cttacctgcc ttgtcattgc caagaaaagc atcggatacc
     9121 tggatagctg aatcaagtaa gcccccgggg ttatttctta aatccaatac taatcctttt
     9181 aatttcccgc ctgcttgttg ttttaattgt tcgattgctt taatcatgtc ttttccagtt
     9241 aaagcctgaa attgagtcaa tcgaatgtaa ccataacctt cgcttagcat tttgcttttt
     9301 acacttttta tttgaataac ttcgcgaatt aagtcaaaag ttaaaggttt gtttacacct
     9361 tttctcaaga tagttaattc tatggtagtg cctggtttac cgcgcattaa attgacagcg
     9421 tctttaagag aaagaccttg aactgactct ttacccagtt taataatata atcgccggat
     9481 ttgattcccg ctttaaatgc tggagtgtct acaagaggag tgattacttt aacaacacct
     9541 tcttccatgg tcacttcaat acctaaacca ccaaattcac cgctggttga tgtttgcaac
     9601 tccttgaatt cctcttcatt gagataactg gaatgcggat caaggccggt tagcatcccg
     9661 cgaattgcat tatcaaataa ttctttatca tcaactggtt tgacatagta tttctttatt
     9721 tcaccgatgg cattggaaaa ccgttgcaca tcttccaacg gaattcgttt tgtattagaa
     9781 taatttgaat tggtttcttc agctgaaaac gcggtcaaag gcagcatcag agttagggca
     9841 taaaccaatg ccaaagtact ggaaaaaagt ttcttaataa ccatagcgat ctcctcagat
     9901 gcaaggccat gaaattagcc acctgcgtat ttttttatgc aatttaaatg ccagcggcta
     9961 agctttaaga caaccagtct aacggattga ccgcttttcc tctttgtctt atctcaaagt
    10021 atagaccgtt ttgcttaatt ccaccggtat ggcctacact tgcgatttgc tcgttttgtt
    10081 ggacgatttg tcctttgcgt ttgaagagag attgattgtg tgcgtataaa gtcataaacc
    10141 cttggccatg gtcaataatc agaagcaaac cataaccttt tagccaatca ctaaacacaa
    10201 ctttccctgg gtaaacggca gtaactatgg agccttcatc ggcaaaaaat gtcactcctt
    10261 gattcatcct gcgtaaagat ctatgggcgg tttggacagg taacggcaat tttttgcgca
    10321 tccccgtgaa cggtttgccg ctttgaatta cactttgttg agataatgac ttcatgagat
    10381 gagataagtt gtctctatct ttttgaaaat cttttaatga ttgttgatta ttttgaatgt
    10441 cattatcaat ggattgaatc agtgtgctat ggtatctttt attttgctct agctgttttt
    10501 gattttgtga caacttaaat tgtagctgct tgttttctgc aagttcattt ccaagcttgg
    10561 ttttactgtc gttaagattt tgtcgggttc gagcaatttc atcaatcaac tgctgacgtg
    10621 atcttattat atactgataa taggttaaaa ttcggttgat cttaaaaggc tcatcttggt
    10681 taatcaacca tttcaaaggt tgatactccc ccatctgata acgtgccctg acatgatttg
    10741 ccagcatttc ctgctgagct tgcaatcgtt tatttaagtg atttattttc ccttgcagtt
    10801 cggcaatgct tttttctttt gcggacatat ttgcttgtat gattcttagc ttgcgtattc
    10861 cttctccaat ttgcttttct gtgctgccca gctcttggtt tagcacagtc cgtttatcct
    10921 gtgcattggc taaaacttgc tttagtttgt taatcttggc atctaactgt tttagtttgg
    10981 tttgtgcttg agttatagaa tttggagcag cttttgcctc aagaccatta caaaaaaata
    11041 gaatgaccat aagtgccaaa ccaagaggag cactggataa atagttgggt aggctaggca
    11101 tgcgcatggt ttttttccag aaggatacga ccagtcattt ccggaggcgg ggttatccct
    11161 aataatgcaa gcaaagaagg tgctatatcg attaaacttc cttcggaatg agtgaagtgc
    11221 caacccccac ctacataaag aaaaggaaca ggttcactag tatgtgctgt atgagcttga
    11281 tttgtcgctt cgctaaacat ttcctcagca tttccatgat cagcagtgat caataattta
    11341 cccccattgt ttttaagggc ttgccaaacc tgctgcatgc actgatcaag gcattcaata
    11401 gcttgaacag tagcttcaaa atttcccgta tgtcccacca tatcagcatt ggcataatta
    11461 caaataatga catgataatc tcggctattg atggcggcta ttagagtttt agttaattca
    11521 tgcgcactca tttctggttg cagatcataa gttgccactt gcggagaggg taccataatg
    11581 cgttcttcat ttgtaaaaac actttcacat ccaccattaa agaaaaaagt aacgtgagca
    11641 tatttttcag tttcagcaat gcgtaattga ctgagaccat gagaagacaa aacctcaccc
    11701 agggtattat ttaaaggaac aggtgggaat gctgtcgtgg taaataaatt tttatcatat
    11761 tgggtcatgg agacaaaata ggacagcttt ggcagtgttt ttctttcaaa tcctttaaac
    11821 aaaggatcaa ggaatgtaga ggttaattgc cttgctctgt cagcacgaaa attgaaaaac
    11881 aggacagcat cgccatcttg tatagaatgc tctttcccta taagagtggg tggaacaaac
    11941 tcatcagaaa ttttattttt gtaataaaaa tgtattgcag tttctgcatc aggaaattgg
    12001 cgctcacttt tgccttgtgt taacaaatta taaacaggct ctactctctc ccaacgtttg
    12061 tctctatcca tcgcataata tcggccacaa attgaattaa tggttgcaac agggtgttta
    12121 gccagttctt cattcaggca tttcaagcta tcaagagcac tttgtggtgg ggtatctcgg
    12181 ccatcaagaa acaaatgcag atggacggat ctgaattttt tttgattaca aagggtaagt
    12241 aaagcaaaca gatgttgttc gtgactgtgc actccgccgg gagagagtaa ccccatgatg
    12301 tgaagagatt tttctgtttt ctttagttgg tcaattactt catggaaaac agcattttta
    12361 gcgaattttc cgttatttat cgcttcatta atgcgagtaa agtcttgctg tatcactcgg
    12421 cctgcgccaa tgtgcatatg cccaacttct gaattaccca tttgctcatc aggtaaacca
    12481 acaggcaagc cggaggcttt caaaagaata tgagggcagg tttgccacca ttcatcccac
    12541 tgaggggtat tggcttttgc aatagcattg tatctatcat tttcgttata gccccagcca
    12601 tcaagaatca tgagtaccag gggagcatta tgcgacatga cctaaatcct aaattgtaca
    12661 atgaaagggc taaaatgata attatacctt cgcggcagta agaggtatat agtttcagta
    12721 agagttcgct gttattaaat ttagtagcaa tcttcttttt gttcgcagtg accataagca
    12781 tcgcatgact ggatcggtcg acacccaggg taataaataa tgggtacagg agttgtaata
    12841 attaaaggag gagtagttga tttatcacag gatttacaat gattaaaact ggctcttaca
    12901 tcattctctt tgggtagatt ttttggattt ggtcctatcc ctaatagatt gaacgccatg
    12961 gcatgattaa caagagcagt taaactgata aataaaataa ttgctttgag aatcaggttt
    13021 ctagtatccc tactcatgat gtgaactcct ttttaatttt atgccccgta ttatgcgata
    13081 ataagagtat agtttcttgt tatggttctg ctactatacc aaaggattat cttgcaattc
    13141 ttccggtaca tattgagaca aatatttgct taaactcaat tgcacagtcg aggttatccc
    13201 atattgcgtt tttaacaata ataaagtact ccagccccac caactgtttg tccaatgcct
    13261 gtcataagga tttgtcaaat tggttcttct taacaactgt ttgttttctt tatcccaata
    13321 gaacatagga gcaatatcca tgttattttc atcatttaat tttgtgatgt gtggaatcat
    13381 gtcctctatt tgattttttt ctggtagaac gcctgagtaa taagcttcaa atatttcatc
    13441 agcggaagct tgcaaggctt ttaagagcat ctgctggttg gtttgattaa aaggattaaa
    13501 gtaagaataa tcaccataaa ccatccattg ttctcccaga ttattcgtca catgaattcc
    13561 gtacttattt tcttcattat gcatataatt ggataacaac gcaccaagga ctgttggtgt
    13621 gataatggct gataattctt ctctaggggt gcgcaaatgg ccggcagcga agtgatctga
    13681 taaatagtgg caggcaaaag catccatcgc ataggcgatt tccaaatcag agcgttttcc
    13741 tgttcgtttt gcttttaatg cctgttgcag tgcgacatgg tggccacttt tataggctat
    13801 cagattattt ggcgtaaagt gatcatagtt ttccatggct agttttagat atctgccagg
    13861 atttaaccac caggtcaggc tattacaacc acctcccgtg atgcagttga tttggcgacc
    13921 tacttcattc ccaattcgtt tataaatggc ttcagcagtt tctccttttg caatccctga
    13981 ttcgacttcg cgcatttctg ctctgataac ggaattaaga tcttctacct cactaactgc
    14041 aataacattc ctggcaaaac tgttgaaaac ctctgtaaaa cgagtttgtt tttccagttt
    14101 tgttaagcca tgactaattg gttttcctac aattccatac agatcaccaa gacttatgat
    14161 atcgccggct gttagtgcca atccattttt taaatgtaat acaacacccg attgaggaac
    14221 ctcatttatg tcaaatcgta gtttgatttg ttgccccatg gaccaatgct cggacaaagc
    14281 aaaattgccg ttcgattcac cataagttat agaggaaaaa accaacaagc taatgctcat
    14341 tgcaaataac cggggcttta cgaatcgatg gatagatttt ttcattatgt cctcatcaac
    14401 agtgattgat cattgggttt agtgagaaat agcgtattgt ataacatttg agatgaaata
    14461 ttgatgcatt ggttcgtccc gagataatgc ataggtatga atggagtctg tacaaataac
    14521 cgtaaccaaa aacgacttag agcgctatag gtcatgggaa ttatctcctt tctgggtatt
    14581 catgatgaaa agtgacaact cgttacgaag atttattcag gtttaataat gaggtgtttc
    14641 ataaacaagg tcaattgatt tcttaagtag tacttaaatt tttatctcaa agaggttaga
    14701 ctatgcatca tttttattag tcggataagt tgttttgaat cataaattac ctcaacacat
    14761 tgccgttgtc atggatggaa acgggcgttg ggctgaaagc cgcggtttgc cccgtgtaga
    14821 gggacacaaa gcgggccttg actcagttaa aaagattatc aattgttgcc tggaaaaaaa
    14881 aatttcctgc ttaagcctgt ttgcatttag ttctgaaaat tggtcaagac cagtgacaga
    14941 agtcaatttt cttatggagt tgtttttgga ggctttgcga aaagagatag acgatttaaa
    15001 ccagcatgga atacggttaa agtttactgg cgatagagaa cctttgtctc aagtattaca
    15061 aaagcaaatg tgtgatgcgg aagctcttac caagaataat caacaattaa ttctcaatgt
    15121 tgttgtgaat tatggtggga aatgggatat agtgacggcg gctagaaaat taatacgcac
    15181 ggttttggac gggaagttgg catatgatga aatcaatgag gcagtattag ctcaatttct
    15241 tgatacgaat ggcatgccag aacctgatct gtttattcga actagtggtg aattaaggat
    15301 aagtaatttt tttctttggc agttagctta tactgagctg tattttactg atgttcattg
    15361 gcctgatttt aatgagcatg agtttgaatt ggcattgact tcttttgcca agcgggcaag
    15421 aaggtttggg caaatttcac aatctgaata aaggtaaaac catgtttatg caacgattga
    15481 tcacgacatt aatattagcg cccctggttt tgctagctat tttttatgct ccttcctgga
    15541 ttttatgggg cgttgttcta gttatactcc tgcttgcagg aatggaatgt ttcaaattga
    15601 ttcctgttga caatttaact gtacaggctt gttttttgat atttttattg atgtgttttt
    15661 gggcctgtgg ctatttctat ccctattggt tgagtgtggg cttattggtt tggttattaa
    15721 atgggttagc aattcttact tatccgcgct cacaaaatta ttggggttat ccaatcgttg
    15781 ttgccatagc ttctttgata ctactaccgt tatttactca aagtcttatt caaatttctt
    15841 ttttacagca aggaaaggaa ttacttgtct atttgctttt tttaatttgg gcttctgata
    15901 ttggtgctta tctcactgga aaacaatggg gaaaacataa attaatccct aaagttagtc
    15961 cgggtaaatc ttgggaaggt gttacaggcg gagttatttt agccatgata gtcgcgactt
    16021 tgggctatta ttattttata ccttactcaa tttatatatg gtttggttta gcattgtcta
    16081 ctgtagttat ttcaattttt ggtgatttgt tcatcagtat attaaaacgg cgttgtcaat
    16141 taaaagatac tggaaatatt attccaggtc atggcggtat attagatcgg ctagatagtc
    16201 taattgccgc gttaccatgg ttttactttg gattaaccta cataccactg ggaatttaat
    16261 tttctggtcg tatcaatctt tggataacga tatgctttca acgttgctgt actttttatt
    16321 agcattggta ttgttagtca ccatacatga atacggccac tttcaagtag cgcgatggtg
    16381 tggtgtgaaa gtgttaaggt tttcatttgg ctttggaaag attcttgcgc gtttttatga
    16441 taagaaagga acagagtatg cctggtcttt gtttcctttg ggtggctatg tcaaaatgct
    16501 tgatgaaaca gaaggagaag tttctgaaaa agaaaagcct ttcgcattca ataatcaatc
    16561 tgtttttgtc aggatagcta ttgtagtagc aggaccttta ttcaatttta tttttgcgtt
    16621 tgtagcattg tggctggttt tggttatcgg tatgcactct ttggctccga tgattgagtc
    16681 agtaaaacca aatagtattg ctgcacgagc tggcctagtc cccaagcagg aaatccttgc
    16741 tcttaatgga gttaaaatta atagttggcg agattttcaa tatgaaataa tgcctcttat
    16801 cggctcccag gaaacagtga gcttgactgt aaagtcaatg ataaatggtg aagaaagcac
    16861 gttgtatttg cctttggata agtggcagtt ggatgataaa aagcctgatc ctattaaaag
    16921 cctgggcatc gttcctttta tacccactat tccacccata gtcggtgaag tggttcccga
    16981 ttctccggct gaaaaagcag gcttaaaaat aggggatgaa attattagtg ttaatggtca
    17041 gcagttcaat gattggttgt atttagtcag ctatgtaagg gaaaggccca attctcaaat
    17101 caatttggac attaaacgcc aaggtaaatt gctgaagata acggtacaca ctggaagtca
    17161 ggataataat ggaaaattag aggggttaat aggagttaga tctcagaaag tagattggcc
    17221 tgctcattgg ctacggctgg agcaacagcc gccaatttca gcactcggta cggcttttaa
    17281 gcaaacagta caactcacag gcacaacttt tatcttaatg ggaagattag tgactggaaa
    17341 attgggatta aacagtatta gcggtcccgt cggtatagcc cagggggccg gcgactccgg
    17401 acgcggagga ttggtatcct atttattttt tctggcttta gtaagcatta gcctgggagc
    17461 gcttaattta ttaccaattc ctatgctgga tggcggtcat cttttgtatt atgtcttgga
    17521 aatcataaga agaaagccat tatcggacgg agttaaatct gtcgggatct attttggatt
    17581 attactgttg gttgccttaa tgtttgttgc tcttagtaat gatatatcaa gattaaccag
    17641 ttaggatttg gcgagagtaa ttctaatttc tgtatgatta atgaatgaaa tgatgtttta
    17701 gcgacaaaga aacttgacag tagtttctag ttcctataaa agggtttcaa ttttttgtac
    17761 aattgaatgg tttttaattt cataagtgcg cgtagtgctg gacgtaaaat aataatgaaa
    17821 aaagtcagta ataaattaat attaggtgtt tgttgttcta ctcttttagc ttggtcatcc
    17881 caaacctttt cttctgatac ctttattgtc aaagggatta gagttaacgg attacaaagg
    17941 gtttcgacag gtacggtatt aaactatatg cctgtgcaag tgggtgagga aatcagttcc
    18001 agctcaacag ctcaaattat ccgcgctctc tatgagacag gatttttcca gtccgtttcg
    18061 cttgaacgcc aagggaatgt gttagtggtc aatgtggtag agcgagcaac tattggctct
    18121 attaccgttg taggaaataa ggaaataccc tctgataaaa tgaaggcttt tcttaaagaa
    18181 atggggctgg tcaaaggcag agtatttcaa agatcttcct tggagcgttt ggagaaggag
    18241 ctgaaacagg cctacacagc cagagggaag tataattctc gtattgaaac taaagtaact
    18301 cctcttactg aaaatagagt agccattagt attactgtat cagaaggtcg agtttcacgg
    18361 attaaagaaa taaaaataat tggtaaccat gattttaaag caaatgagtt attgcctgaa
    18421 ttgacgttaa gtacaagcaa tctgtctact tattttacta aaaaagatca atattccaaa
    18481 gcaggaatgg atgcttcttt agaagcatta cgttcatttt atttagatag gggatatttg
    18541 aaatttaatg ttgtctcttc gcaagttttg ctatcgcctg ataaaaaaga cgtctatatc
    18601 aatattcata tagaagaagg acctcaatat catttctcag gttatgatgt ggttggaaaa
    18661 acgatattac ctaaagaaaa aattgattca ctgatacagg ttaagaaagg cgatgttttt
    18721 tctcgtaaaa aggttactga atccatctct gcaatagggt tagctttagg ggatgtagga
    18781 tatggttttc ccgcgattaa tgctgaacct agaatagatg aaaataataa aacagtattt
    18841 attacttttg tagtgcagcc gggtcgtcat gtttatgtga gacgtattaa ttttcatggt
    18901 aatacgaaga caggagatta tgttctacgt aatgtgattc gtcaggatga aggtgggcta
    18961 ctgtctttac ataacatcaa ggaatctgag cgtcagttac gaatgctggg ctatctaaaa
    19021 aatattgatg tcaaaacgac gcctgttcca ggaaccaata atcaggtaga tttggatgtt
    19081 aatgtggaag aagctccttc agcagaagca agcgcatcca tgggatatgg tacaaacggt
    19141 tatcaattta atgcatccgt taaccaacgc aattttatgg gaagtggacg ttccatggga
    19201 gctgctttca atgcaagtca atgggggcaa gactactctt ttaactatta taatccgttc
    19261 tataccgata ctggggtagg ccggggagga agcttatatt attcaaggat tgatcctaaa
    19321 aatttaaatg tcagtacata cagttctaat cgctatggtg gcgacatcag ctataacttc
    19381 ccgttaggtg agaaaagcag ctttcaattg ggatatggtt atcaagatat caatattaag
    19441 tcagtgggtt acgtactccc cataatcaac tttgttgctt taaacggtaa tcattttcag
    19501 gaaataaggt tgacatccgg ttggagtaga aacagctatg atcaaatgcc ttatcctaac
    19561 cagggattta atcaacaagc tattgctatg gtcgcgttgc cagcaacatc gcaatcttta
    19621 tcttactata agagctctta tcaggcgcat ttatattatc ctctaacccg cggctggatt
    19681 ttttctgtcc tgggtaatgt ggggtacgga aatacttttg ataatttcgg attacccttt
    19741 tttgaaaact actatgccgg tggtccagtt caaccaggcc aggttcgagg gtacgatagt
    19801 tattctttag gcccgcaaga taattttgga aatgcaatgg gtgcgaattt ccttgtgaat
    19861 ggaagtgtag gtttgatact accataccca ttgagtcgag ataatgtaag aacgactatt
    19921 tttgctgatg cgggtaatgt atttgcttcc gggacacctg ctgctttacg tggaactccg
    19981 gcaggtccga tgcgttattc agctggtgtg tcattagaat ggcgctcacc ttttggtcca
    20041 ttgtctttta gcttggctaa ggcattgaat ccacagccat tggatcagac tcagctcttc
    20101 caatttgctc tttcctcagg tttttagagg tattatggat tagagtaagc agattttagt
    20161 attttaaaaa ctagtgctgt acatatcgca acctttttcg aattgtgcta gcatttcaaa
    20221 ctttaaatta ggagatcatt atgaaacgtc tgggtgcggt gctagtcgca ttatttttgg
    20281 gtttgttcgg cacgactgtg tttgctgatt gggcaaagat aggcgtagtt gatttacaaa
    20341 aaatcatgca aacctcaaat caaatgaagg aaatccaaca gaagttggaa aaagaattta
    20401 agcctcgtcg agacaagctt gtcgcaatgg aagcaagctt gaaaagcgac atggaaaaat
    20461 ttaagcgtga tagtgctata atgagcgcga gccaaaagaa agaattggaa aagaaaattg
    20521 ttgcgtcaca acaacagttt gaacgtgatg gacaacaata tcagcaagaa ttgagcacgg
    20581 ctcacaatga agctatggaa ggtttgtata ataaggttcg tactgccatt accaaaattg
    20641 ccaaggatga gaagtacgat attattgttc agaaagatgc agcgcccttc agcagcgaat
    20701 ctcttgatgt aacagataaa gttatcaaag caattaatta atttggcgga tgcgttgcta
    20761 accttagagg aactggcaaa ctatttaaat ggcgtctggt atggcaatgc caatcacgcc
    20821 atttttagtc tatcctcgtt ggctcgagct tcatctaagg atcttgcgta ttttgataat
    20881 cccttattgc agtctgtctt aaactctacc agagcaggtg ctgttttatt aaagcccgag
    20941 cacaaagatt ggtgtcttgt gaactgtatt gtagttccca atccgtcaga agcaatgttt
    21001 aaagcagcaa agatcctgcc tcaccttgct accaatgttt gcggaattca tcctacagct
    21061 caaatccaca aatctgccca aataggtcaa cacgtatcag ttggcgctaa cagcgtgatt
    21121 ggtgaaaatg tacaattgga tgattacgta actatcggtt ctaatacgac cattgaatct
    21181 tcagtactga ttggcagagg aagtcagctg ggttctggat caataattca ttctggtaca
    21241 gttttgggcc aatctgtaat tattgattct gggtgtattg ttggtgctgc tccgtttaat
    21301 tgctacaagg aacatggcgt ctggcaacaa gcaccaaatt ttggtggagt tgtaattggc
    21361 caaagaactc aaatcggtgc aaatacagta attcacagag gctccattgg cgatacttat
    21421 ttaggcgatg gagtttgtat agacagttta gtactcattg cgcatgatgt ttatataggg
    21481 aataacactg ctattgcagg ttctgctgcg attggtgctt tagtgcaaat aggctgtgat
    21541 tgtatcatag gtggggcaag ttgccttgca gcgaacattc gtttgactaa tgatgtagta
    21601 ataacaggaa tgtctacagt gacaaaatcc atcgctcgcc ccggggttta ttcttcggga
    21661 acgacggttc acgatcatcg caagtggcgt cgtaacacag cgcggttcag gcatttggat
    21721 gactacatta caaagctgat aacaatagaa aaaaaattga ataataacaa ctaattatgt
    21781 tttatcaatt tgaagtaata ttatttttga aatttttaat tgattagaat gagctaaacc
    21841 aagataatga gccattttgt aatgcaatag gcgattaatg agaactaggg tatgaacgaa
    21901 tcgatagata taaatcaaat ttttactttg ttaccacacc gctacccatt tattctggtt
    21961 gatagagtga tagattacaa agtaatggaa tatttgattg caatcaaaaa tgtgactatt
    22021 aatgagaact tctttaccgg gcattttcca ggaaatccta ttatgcctgg ggtactaatg
    22081 cttgaggcct tagcccaagc ttgcggcatt ttagccaatt tgtctcgcca acccaaagaa
    22141 gggtatgagt ttctgcatta ttttgccggt attgacaatg ctcggttcaa gcaagtagtg
    22201 attcccgggg atcagttgcg attagaggtg agactggctg gacaaaaaag agatttttgg
    22261 cgtatgcacg gagaagctta tattggagac aaattggctt gttccgcaga cttattaagc
    22321 gcagcaaagg aaattaaaaa gtgatagatg aaagagcaat gattcaccca tcggcgaagc
    22381 tggcaagcgg agtgtcagta ggccctggga ctgtgatagg cgctgatgtt gagattggtg
    22441 aaaatacctg gatagggccg catgtggtaa ttgaaggacc tactgtaatt ggtaaaaaca
    22501 ataagatctt tcaatttgct tccgtaggag atgagccaca agatatcact tacaagggcg
    22561 agccaactcg tcttgaaatc ggcgataata atgtaatcag agaatattgc atgattagcc
    22621 ggggtactgt caaaggtgga ggagtgactc gcattggcaa tagcaattat ttaatggctt
    22681 attcacacat tggccatgat tgtatggtag gcaatcatat tattatggtc aattatgcag
    22741 ccttatcagg ccatgtcaca attaatgatt acgcaattat cggcccttat gctgcagttc
    22801 accaattttg tcaggtggga gcctacgcat tcatagcccg agctacctat gtcactaaag
    22861 acgtattgcc ctatgttatg attgcaggtc ataccacttc tgcttgtgga atcaacactg
    22921 ttggtttaag aaggagaggt ttttcttctg ctgcgattga ttgtttgcga cgcgcatata
    22981 aaattatctt tcgcaaaggg ttaaccgttc aacaggcagt ttcagaatta gagttgattc
    23041 aaaatgagtg tccagaaatt attcctatga ttgatgcttt gaatcaatcc acaagaggca
    23101 ttgtgaggta agcaatggtt gttgcaccct atttggctgt agctatcgga ggctctttag
    23161 gcgcaatgtc tcgttattta gttacaataa tggctcaaaa tgcctggggg attaaatttc
    23221 cttatggtac tcttttagtg aacacactag gttcttttct agctggtttt tttcttattg
    23281 tattagtagg tcgttttagc gccgaggagt cgtttcgttt attccttttt acgggatttt
    23341 taggagcttt tactaccttt tcgagtttcg ctgcagaatc tttatttatg tttgagcaag
    23401 gatattggtt taaattaatt accaatatac tggtcaataa tgttggctca ttgtccatgg
    23461 tcttcattgg aacattagtt gctaaatatg tattattagg acatcagggc tccaattaat
    23521 aatttttaga gtgttggctt atgttagata atcaattact tcgagagaat cctcaatatg
    23581 tagcaaccca attgctgaag cgaggatttc aatttgatgc ggtcactttc agtcagttgg
    23641 aagaaaagcg taaggctttg caagtaagta cgcaatcatt acagaacgaa cgaaatttgc
    23701 gttcgaaagc catcggtgaa gcaaaatctc gaggtgaaaa catagaacct atgcgcgaag
    23761 aagtgaataa acttggcgct aaactggagc aacaaaagac tgaactggac gagattctta
    23821 agcaaattga ggtaattagc ctatcattac ctaatattcc tcatgaatca gtacctgttg
    23881 gtaaagatga attggataat caggaaatca gaaaatgggg cgacgtacct gctttttctt
    23941 tccctgtaaa gtcacatgat gaattgggtg aagcactagg gcaaatggat tttgcgcttg
    24001 cagctaaaat tacgggcagt cgttttgtcg tcatgaaagg gcatctggcc cgattacatc
    24061 gggctttaat tcaattcatg ctggatatac atattcagca acatggttac caggaaatat
    24121 atgtacctta tattgttaat gcagatagct tactagggac tggacagttg ccaaaatttg
    24181 aggctgattt atttaaatta acaggagata atgggtacta cttgacgtct acttcagaaa
    24241 ttccagtgac caatacagta agggaaatga tattgtctgc tgagcaatta cctattcgtt
    24301 atgtttgcca ttccccgtgt tttcgtagtg aggcgggttc ttacggtaaa gacacaaagg
    24361 gaatgattag acagcatcaa tttgagaaag ttgaactggt ctggattacc aaaccggaag
    24421 attcttataa tgctttagag caacttactc agcatgctga agttatttta cagcgtttga
    24481 acttgcctta ccgagtggtt gctttgtgta ccggggatat aggcgctggt tcagccaaaa
    24541 cctatgatct tgaggtttgg ttgcctagcc agaatactta tcgagaaata tcctcttgtt
    24601 ccaatatgga ggcatttcag gcacgccgaa tgaaagcgcg tttccgcaat ccggatacta
    24661 atgagattca attagtccac actctaaatg gatcaggttt agctgttgga agaactttag
    24721 ttgccattat ggagaattat caagatgagc atggaaacat tcatataccg gatgctttga
    24781 agccttattt aggtggtatt gatatcatct ctgtgaaata ggtttgctca taattatgga
    24841 tattttttct taaaagttag ccttcaatag gaaagagcga tagataggct atattgagcc
    24901 aaatcaattg ctcttaagta ttt
//
LOCUS       DASLOE010000029        24059 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.029, whole genome shotgun sequence.
ACCESSION   DASLOE010000029 DASLOE010000000
VERSION     DASLOE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 24059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24059
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.029"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            207..788
                     /locus_tag="U6309_000961"
     CDS             207..788
                     /locus_tag="U6309_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEN5504414.1"
                     /translation="MSLDNDSLHLPKYDDFVQSISVLALTMSGSELHGIMCGYLCAGA
                     DSQGEAYIRALLNNKKDEQSRNALLSMFSVFSISQQQMNNFDFEFEMLLPDDDESLIT
                     RAQAFSEWCEGFTQGLTIAGVGMEQFYEEESQDALQHLIEFAELDCESLEVGEEDERA
                     LMEVSEYTRMAVLRLHSDLVLHERELGDSGTTH"
     gene            893..2203
                     /gene="pepP"
                     /locus_tag="U6309_000962"
     CDS             893..2203
                     /gene="pepP"
                     /locus_tag="U6309_000962"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEN5504415.1"
                     /translation="MISQQEYKARRKKLAAQLPDDSVAVIPAAYEVLRSGDTHYRFRQ
                     DSDFYYLTGFNEPDALLVIIAGKDSQSILFNRPRNPAEEQWTGKRLGQDGALQELGVQ
                     AAYPLSSITEELPKLFSGKSAIYYDLGRNLSLEKKIRHVLSSLKAQVRRGIKAPESLC
                     DLEPILSEMRLLKSDAELELMRQAARISVAAHKRAMQVCQKMEYEYHLEAELIYEFSR
                     HGCRSVAYDPIVGGGANACILHYTENNKPLRRGDLVLIDAGGEYGSYAADITRTFPVS
                     GTFSPEQKIIYELVLKAQKAGIAAIEPGLPWNHIQQTIIRILTEGLCDIGILKGDLEE
                     LIKNEAYKPFYMHNSGHWLGLDVHDVGRYKINNEWRPLEAGMVLTVEPGLYISAGMEG
                     VDKRWWDIGVRIEDDILVTRAGYEVLTGDLPVNVDDIEALMRGE"
     gene            2193..3395
                     /gene="ubiH"
                     /locus_tag="U6309_000963"
     CDS             2193..3395
                     /gene="ubiH"
                     /locus_tag="U6309_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HEN5504416.1"
                     /translation="MANKETDILIIGGGLTGASLSLALQGLGFSVTLVESRPYSDKVN
                     PDFDARSLALSPASQRILAMLGVWEILKEYTTPIHMIHVSDQHRFGASRLHGEKNSPL
                     GYVVEMQYINRALSILINKEHLLAPAALKSIDFNNSTATVAHGDDMISIQAQLIVAAD
                     GTESMTRHLCNLPVKIKDYAQDAIVANVGLIKPHGNRAFERFTPNGPLALLPMMNDKM
                     SLVWASKPEEAKRLVMLNDGDFLRELQTAFGYRLGRFGKVGKRYSYPLKQVLMPQQTK
                     WPVVFVGNAAHTLHPVAGQGFNLGLRDIAALAQCISQKGLNPQMLEHYLKLRAHDQQM
                     ITWLTDGLIHLFTSRLPGMGVARNLGLLALDNIPALKNLLARYARGFGGITPDLVCEI
                     ALSTGEFQ"
     gene            3392..4555
                     /locus_tag="U6309_000964"
     CDS             3392..4555
                     /locus_tag="U6309_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HEN5504417.1"
                     /translation="MSQHFDVLVIGGGIVGLTAALAMAQRHYTVAVIDAGTLMVNTDK
                     SDLRVYAINKASKMLLSELGVWQHLDDARVAPYNKMHVWDGVSGAYIDFDSRSIAEPN
                     LGAIIEESILKQALLRRISSESKINLFPQSVIDEVCHLERSIKISGQNTAWEGQLLMI
                     ADGANSPMRHKLKVDVTSWSYEQQALVATVNSEKSHQNTAFQVFHPDGPLAFLPLSNL
                     NQCSIVWSTHADNAMRLKLLDDNEFSRELTKAFGGQLGQIKVESNRHQFPLQMRHVKQ
                     YAGHRWLLLGDAAHTIHPLAGLGLNVGLADVKSWLTCLEMGKGELISKKVLGAYQRER
                     KHSVWQIILLMEGFKRLFGTSASPVTSLRSIGLRACNEFTPIKRLFMRQASGL"
     gene            4732..5499
                     /locus_tag="U6309_000965"
     CDS             4732..5499
                     /locus_tag="U6309_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504418.1"
                     /translation="MTRLKFFSKFQKALLQLETNVSNLSNPLVGSKIKELQTKKILVR
                     ADGRSLDHLYKLGGLPQRVESEDLEKVRITDVEKYQKFNVNPFGWGACASAEDLRKFL
                     ENYSELTKQAWVHKFYGSSTSLLTLKSEVGISCGDHDEEKEELIVDSVSFEQIIASTC
                     PKYREKYLDGGLVPNAMKDFKPKVPHTMRLGDFSSEKSTLEWLLINDCEEEFAKLCKE
                     IYGDTPLKILLMRFEGDKYLADAVTYYVKESSSSPRI"
     gene            complement(5489..6814)
                     /locus_tag="U6309_000966"
     CDS             complement(5489..6814)
                     /locus_tag="U6309_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5504419.1"
                     /translation="MRSKLFSFFKRASRPMKATPPKIWFETQLKSRGLDKKFQIDELS
                     ETQSSVRVFANKKYLPDTETINEALIKIAAIDVSGDKSGFFQNGLPFPDEAGYFEKIP
                     LGHPELLSPIERLTGSKKVVSSHSLVTASGGYPLTNPLVPYRKPIKVCVFSLAGPSFE
                     NNYLHYSLFLLDPVQKIIDSPLFSHLYDGLPIQFDEAKKELGEYDTNKMIARIRLGFP
                     YLARFSSGGFYPSFSKTNAIIFLSEAYFRYQLEDISLLLASVNQTAKETGKKAFLKAT
                     AVGMGFFAKIDCGYDIKHMIFPYYLRAYKKLLSEHKFPWIAKIEFPIFNEIQQEQFDS
                     IFEDYNGLTKVYQSTRDVLQFREEEIEKYLPAVINPSDAFALTGNEWGYASVESMIGN
                     NSSIRFDQVHHMNPLILDPSHHVEAQINKDHSVELSIKSTPRSLSSIKF"
     gene            7099..7557
                     /locus_tag="U6309_000967"
     CDS             7099..7557
                     /locus_tag="U6309_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504420.1"
                     /translation="MFRRLWLLRPVNAPALDLSRMALGRMPAETTYESIKNDFEQELH
                     RVGLKQFQENIHAEQLILEQLLKQREEITKKIDEKCLRPALQRSANGYDVKGLYWGKI
                     QEDLSKIQSKKERVEMDEMIDWLCARRRFLKERNNLSLDKEPDHPASLLL"
     gene            complement(7554..8501)
                     /locus_tag="U6309_000968"
     CDS             complement(7554..8501)
                     /locus_tag="U6309_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HEN5504421.1"
                     /translation="MINLFRRLKNHGVLSINQRNTDFVLRYNPRKLFPLVDDKLKTKK
                     LALKAGIAVPALYDIIETEQQIKSIETRFASYNDFVVKPARGSGGDGILVFKDKVYGR
                     YRQINGKLTTVQELSYHLSCLLSGAYSLGGSSDYAIIEKRVVVDPVFAEVSYEGIPDI
                     RIITLLGYPAMAMVRLPTRLSGGKANLHQGAIGVGVDLATGKTLGGVFQNDTIDYHPD
                     TLNPIVGIEVPYWNQILEIASSCYELTGLGYLGVDIVLDKEHGPLMLELNARPGLNIQ
                     IANREGGLKRYRTIEARFKESGQESTLDKVSFCRKEFAR"
     gene            complement(8498..10021)
                     /locus_tag="U6309_000969"
     CDS             complement(8498..10021)
                     /locus_tag="U6309_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive transglutaminase family protein"
                     /protein_id="HEN5504422.1"
                     /translation="MKNNKRHVYGLILTLLLLGSGIFLYRHIILDVPLTDTETINSWM
                     VESNLRFTADRNTPIKASFNIPYLPPNFAILDEYFVSRNYGVTTNLNGSNRETVWSIR
                     RGHGPQSLYYRAIFRQTDSDESSLPKPSVIKSQPLNDSQKSAVETITNQVRSTSADIQ
                     TFAQSTIKELNKRDGNAKLLVGNEFNDDNIINATILILNQSKIPAITVQGIYLNQQKK
                     ADLKSLLAVFNGKNWIYINPKTGSAGLPKEFLIWQYGNGPLFNVVGGNRAQFSLTVSP
                     TPINALSVAKSRGLEDSQLLRFSLLQLPVNVQGIYKILLTVPIGAFIILILRNFIGIK
                     TFGTFMPVLIALAFRETHVAWGITLFVIIISFGLLARFYLDQLRLLLVPRLAAILTVV
                     ILLMIFISVLCQNLSLDTGMSVALFPMVILTMTIERMCITWDERGASEAIKSGIGSLA
                     AAVISYGAMSYEPLQYLIFAFPELLLVLLSLILWFGQYRGYRLVELKRFKSLASAMK"
     gene            complement(10011..10538)
                     /locus_tag="U6309_000970"
     CDS             complement(10011..10538)
                     /locus_tag="U6309_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease"
                     /protein_id="HEN5504423.1"
                     /translation="MRSKFVLFMLMSLLTGSLMANSEAQIYGYVEKATLIDQNLTLSA
                     KLDTGAKSASLHAVNITEIEKKGIPYLRFTVPTKTGDYSFEGEYVGKVKIKVRSSETN
                     PGLLRTTPIKRPVVLLNIKLGDKVRTIKVNLTNRKRFLYPLLLGRDAIIDFNGAVDPA
                     LTFTTKSKSPVNNEK"
     gene            10788..12713
                     /locus_tag="U6309_000971"
     CDS             10788..12713
                     /locus_tag="U6309_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504424.1"
                     /translation="MPHASDENQASKPTDLVFILAIAGRIDAQSRILAQKLDKSRSIY
                     YAYAVMDSLSSSYSMFKYFFDLFASPNDNDAMHEFLNSPTGLIAIAVESVFLVTFSVL
                     ACQFENEKEDYYKKFIANAWPYFRDVMKGLKNAYKGWRSAVVAFSLIGGVDAKFLILP
                     MGVILGVFAAANRFWIRSMLEDRKTMMSENIALLMEIKKLISLSHEESFSYFKRIRYQ
                     TMQTRNFAFWAVAAGGLLDGLYLYVGVLGLAVLSPQLLMAMALICAFYTVACVITRIY
                     EEYDFQLRLLVTQTKCQLALITKELETNYAKLLLLQDKMDLDKVDQEELKRLKRLVHN
                     LITRFDDSRKLLSQQSNRTYLSSALLGMKNGLYAYGALASVLFLVGSILVMAGVSFPP
                     ALLTACIILGLVLVLSFTIHSLVSNYWHQKKQETQKQRPYERLIEWNNCLNDEFNKTE
                     LLDPEEFHKLLNDGLSLDPSPQFFFQEWFEVLRSLFSGFGKGQKFIDFVGNSLQELGE
                     DGHYRDTPVMYVLSAFSALLFGVTLALRALAKGLGRTPLAQQAKLTEDSKEASVSPEI
                     KKEVMQKTEEQRESIQSEKTRTDSPGHTDSLSLFGIFETKTKRNQPDSPSIPHSKSES
                     ALNQLTPSQNSTILGLA"
     gene            complement(12679..13269)
                     /locus_tag="U6309_000972"
     CDS             complement(12679..13269)
                     /locus_tag="U6309_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HEN5504425.1"
                     /translation="MQESFDPSTITTLHKTSIIRWKANGIEYQHKQFLQLVEENHAFN
                     FQLWHAEDKARRDDMGFEFVYQAKREIDAFNQQRNNRMEAMDEWLYKALQPENHNHCP
                     VNSESPGMIIDRLSILSLKSYHMDLQTKRQDVGVEHRMACTQKLEIIQQQLNQLALCL
                     DELLNEVRAKTRTFRVYHQFKMYNDPKLNPELYCSD"
     gene            complement(13496..14224)
                     /locus_tag="U6309_000973"
     CDS             complement(13496..14224)
                     /locus_tag="U6309_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN5504426.1"
                     /translation="MKRFMFILLIFLNFHAFGKPLIIGVPDFAPPFVSRSGGGEFYFG
                     FNIDLMNSICKIIGEECQYKGMNIQQLYTNLNAGKVDIMLAPTPIVSTAGTNYIFSLP
                     YLPSNAQIVTLKANDHINALTDLKGKKVGTLKYTLYGGLLQEHFQDYVDLMQFSKLSD
                     MAIALSNREIEAVVLNANAAKYAMSNSATELKFVGDPIPIGNGYGILALKNNAPLIMK
                     INKALLQIEKDGTYLEIYNTYFSD"
     gene            complement(14315..14761)
                     /locus_tag="U6309_000974"
     CDS             complement(14315..14761)
                     /locus_tag="U6309_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEN5504427.1"
                     /translation="MTIWVDADACPKMIKDILFRAAIRTNTNLILVANSYLTYPNSPF
                     IRSVLVEKGYDRADHYITSHMKAKDLVITADIPLAAEVIVKQGLAMSPRGELFTANNI
                     KQRLTLRDINEQLRSAGERTGGPSALSAREKTNFANALDRWLAKSK"
     gene            14872..18846
                     /gene="lem1"
                     /locus_tag="U6309_000975"
     CDS             14872..18846
                     /gene="lem1"
                     /locus_tag="U6309_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem1"
                     /protein_id="HEN5504428.1"
                     /translation="MKQLISIIKGQIQQLPIPDLKHLLLSHLKEDAHDLTELFCQQIP
                     AQKNQFSLNHSVPFGLPASLSVLAIVLTYAPNLLPRELSEQLLTTLDSIKETETSVDN
                     QLAERCTEQLNYYSDAKPVLIALKERGAKDLIQILEQADERSLLAFSHLAKDTDLNVL
                     IETFLVKNNKDLDLVNRNHLVQFLRQPDLAGWLTRFLDSLIDESINALSIRLLLSFIS
                     SEKAIEKLKEARACLAQYPFSIDLHAMRAVFDFYQHELKKDLFSPESHLVMQFLHDLL
                     KNTKLDKQQEFFQSLSQEILLLIIEYCIAEYNKTTGEEKQNCKNLLTRLCSAFSDSSQ
                     DFLGKIRTKINQLDPYLFDTPQLYQLASGLITKSCNSGKEQLSSSPIASLLSMPQFVG
                     VCTPKVLKQLVERYSLYTLTLNQKDYKQDLCFKEYAPYFDEINEHMAQIERNLLIHPE
                     AQSFWLDKKYSLLQFRVEISKSALLYQMECLLEANKGGDELNNKGLEVLYHYYALNYP
                     NLRSDLFLKVVNHIYSRFFTKGNNETEARNGLLHWLKQNFPLIDKLPKELDRKKEIFL
                     YNSAGKKIGFLGESNVAMTFVNEEIVLLARTGSMDINEPLYDQEGLVLGYLTEESCLR
                     SESAIHKNAAAWVLAGLSKSDLESSSFGLESLLHNVLFENAIGVLYQCESICLDANKR
                     IWLNNKLLKEIQEAPGEIPASVLYPLLDHFEDETVFELLGVIRNKNNALELFHYILNR
                     DSRRSILLNGLYESDFQRFLEHQGADVCLADYLVNYYDKPWFSEVLLRFAYHGKKYKK
                     PDLLSSALTFITKEISQEKKQETICDAVLDRLINSEACAVLVLKEFLGDRTHNSIQKV
                     NNPEIGKVSQFFDRRHIVPLIAQLNKTSYWEQNAQYKLALHILANQHGRLFSETDLNP
                     DSPQAWQGKELKELACFTSRHLSKKRPFDNKHDIGYRVLGELLFRCANSGITSLFYKQ
                     KTLNRAITRLSITEPFLVRMVDKLWMPKGIRELYGKSLFFKGSWFDDQVFLQKELKDH
                     HPLLDWSHFIKQTWSKRDKQKKSPIICSYLLSYTGKKSVLSHLLQDYFNSFQGMPDYI
                     RPVSQLLRFFPQRDVSAVIYDALEAEMIKKPQLLDRQILSDMAFYYGKQLARKDTSFP
                     QADINLLTYWGQNKHYSLVKRGCELLVRDCEDKGVKKRLVSAVKEAEVEQDLSSSVQT
                     FYFGLIKFFKRLWHYGFNAENSSSKIIKFCDDPTPGPARKKAGDLINAPVAESRTNSL
                     YLGFSERRKQLISLLATIKSSPAHFTRVVNYSDNTQTFFGEQPKKSINYNTPAIPSNF
                     ESIM"
     gene            19067..19531
                     /locus_tag="U6309_000976"
     CDS             19067..19531
                     /locus_tag="U6309_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504429.1"
                     /translation="MRILLSFYLLLLSTGLFAESTFPDGCRAVPVQGQSVTLKAKKNK
                     LVFIHNITNLDLWITHPVTDPGASAGWTSRLQAGKWSALVVDKPAFILNCIESRPGHE
                     QQIPCENAIAVCQWKGVKIPASNQGTFWVAEDLSLAALNAAVGGRGFVLPEK"
     gene            19927..22107
                     /gene="rnr"
                     /locus_tag="U6309_000977"
     CDS             19927..22107
                     /gene="rnr"
                     /locus_tag="U6309_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEN5504430.1"
                     /translation="MSKKSKDPFYKREREKYAEPIPSREYIMEILDEYGRPMSRSKLF
                     DKLDLSSESQQESLGFRLKAMLRDGQIMQDRRGRFCLLQKINLLRGTVQGHPDGFGFF
                     IPDDGSEDMVLSAKEMRAVMHGDVVLAYQSGLDRRGRPEAKIHEVLEHANTTVVGRFF
                     TDHGVNFVIPDSKRLTQDISIPQEFIGEAKNGQIVLVELIAYPNKRSQAIGRVIHILG
                     EHMAPGMEIQVAIHAHGIPFEWPDDVLAEVGKIPQQISEEQLKGRTDLRNLPFVTIDG
                     EDAKDFDDAVYCYKKPKGGFQLYVAIADVSHYVAKDSALDQEAARRGNSVYFPGKVVP
                     MLPEALSNGVCSLNPHVDRLCMVAELSITQEGKISRSRFYRAVIHSHARLTYTSVGAW
                     LEQGKADEKHQALWPMLESLYDLYHVLLDTRRLRGAMDFETTETRIEFDENKKIKCIV
                     PVIRNDAHKLIEECMLAANVATARFLEKAEIPTLYRVHAAPEEDKITALRQFLGELGL
                     SLGGGRKPGPKDFQRTMNMIGDRPEKHLIETVMLRSLKQAQYVESNEGHFGLAYSAYT
                     HFTSPIRRFPDLLIHRAIGHLLDNHPVYEFEYSHEDMNRLGKHSSMTERRADEATREV
                     ITWLKCEYMQDKLGQVFKGRISAVTSFGIFVELDEIYVEGLVHVTSLKNDYYTFDSAK
                     HRLIGARGGQVYRLGDKMTVLVARVDLNERKIDFEPVEDEVCNE"
     gene            22100..22849
                     /gene="rlmB"
                     /locus_tag="U6309_000978"
     CDS             22100..22849
                     /gene="rlmB"
                     /locus_tag="U6309_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEN5504431.1"
                     /translation="MNEQYVYGLHAVSALLANPHRMIKKLFVNQDRIDKRLHEFLVKA
                     ENAHVPIEKLSSQKMNQQFSEFTHQGVVALASALPEYGESDLVTLLDRSKKPVLILIL
                     DGITDPHNLGACLRSADATGVDFVVIPKDKSANITPVVSKVACGAAESIPLVRVTNLA
                     RAMDILKQQGVWIFGAAGEAEHLLYAMDCSGNIALVMGAEGTGLRRLTREHCDGLFSL
                     PMMGSVESLNVSVATGVSLYEVIRQRGQFRE"
     gene            22842..23492
                     /gene="rpiA"
                     /locus_tag="U6309_000979"
     CDS             22842..23492
                     /gene="rpiA"
                     /locus_tag="U6309_000979"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEN5504432.1"
                     /translation="MSELKIKAAKAAIAYIEDDMVIGVGTGSTVNFFIKELAAIKHKI
                     EACVASSKATEALLRAEGIPVIDLNSVQDLPIYVDGADEVNERGEMIKGGGGALTREK
                     IVANVATQFICIVDESKVVKRLGEFPVAVEVIPMARSFVARQIVKLGGDPEYREGFVT
                     DNGNIILDVFNLNFSTPMALEDSLNVIPGVVENGVFAKRLADKVLVASASGVNNLK"
     gene            23556..>24059
                     /locus_tag="U6309_000980"
     CDS             23556..>24059
                     /locus_tag="U6309_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEN5504433.1"
                     /translation="MDTHKVFVNRIINMRKIKLIGLDMDHTLIRYNSKNFESLVYDLV
                     KERLAESFHYPEEIKKFKFNFDDAIRGLVIDSKNGNILKLSRYGAIRLSYHGTKQISF
                     SDQKKIYRSIYVDLGDPNYMAIDTSFSIAFCILYGQLVDLKDTNPDKMPSYQAIAQDV
                     QYCVDKVH"
BASE COUNT         7179 a         4173 c         5196 g         7511 t
ORIGIN      
        1 ttcatagatt ttatttaata attttatttt acagagttta atattggtca ttattacgcc
       61 tttgtatttg cacggtacta agcaaatagt agcaattttg ccttaatgca gcaaattatg
      121 aaaatcatag gtgctgagaa agtgcagcag gtgaacactg attctaaatg gagtatcatt
      181 agctatcttt ttaaataggg ttaaatatgt ctttagataa tgattcgctg cacttaccta
      241 agtacgatga ttttgtgcaa tccatttctg ttttagcatt aactatgtcc ggaagcgaat
      301 tacatggaat catgtgcggt tatctgtgtg caggggctga tagtcaaggc gaggcttaca
      361 tacgcgcttt gctaaataat aaaaaagatg agcaaagtcg taatgcgtta ttatccatgt
      421 tttctgtctt ttcaattagc cagcaacaaa tgaataattt tgattttgaa tttgaaatgt
      481 tattgcccga tgacgatgaa tctttaatca ctcgtgctca ggcttttagc gagtggtgtg
      541 aaggatttac gcaagggttg actattgcag gagttgggat ggagcagttt tatgaggagg
      601 aatctcagga tgctcttcag caccttatag aatttgcaga gctggattgt gagtcgttgg
      661 aggtaggcga ggaagatgag agagcgttga tggaggtcag tgaatacact cgtatggcag
      721 tacttcgatt gcacagcgat ttggttttgc acgaaagaga acttggagat tctggaacaa
      781 cccattgata gcaagttgat aaagtatcga agttgtttgt atttattggt ttctttgatt
      841 ttttaaggat tgctcatttg ttctatttat taaagtgaaa agggaaattg atatgataag
      901 ccaacaagaa tataaggcga gaagaaaaaa attagctgcg caattgccag atgacagtgt
      961 tgctgtaata cctgcagcat atgaagtgct gcgcagtgga gatactcatt atcgatttcg
     1021 acaggacagt gatttttatt atttaacagg atttaatgag cctgatgctt tgctggtcat
     1081 tattgcggga aaggatagcc aaagtatttt gtttaatcgc ccaagaaatc ctgcagaaga
     1141 acaatggaca ggtaaacgat taggtcagga tggcgctttg caagagcttg gagtgcaggc
     1201 ggcataccca ttaagcagta ttacagaaga gcttcctaaa ctattcagcg ggaaatctgc
     1261 catatactat gacctcggcc gtaatttatc attggaaaaa aaaatcaggc atgttttatc
     1321 ttcactaaaa gctcaggtga gaagaggcat taaggcacct gagtcgttat gcgatctcga
     1381 acctattcta agcgaaatgc gtttgcttaa aagcgatgcg gaattagagt taatgagaca
     1441 ggcagcccgt atttcagtgg ctgctcataa acgagcgatg caagtttgtc agaaaatgga
     1501 atatgagtac cacctggagg cagaattaat ctatgagttt agccgtcatg gctgtcgtag
     1561 tgtagcttat gaccctatcg tgggtggtgg cgccaatgcc tgcattcttc attatacaga
     1621 aaataacaaa ccattgcgcc gtggcgattt ggtgctcatt gatgcagggg gtgaatatgg
     1681 tagttatgcc gcagacatta ccagaacttt cccggtaagc ggtactttta gccctgagca
     1741 gaaaatcatt tatgaattgg tattgaaagc acaaaaggct ggcatagcag cgattgaacc
     1801 gggccttccc tggaatcata ttcagcagac tattatcaga attcttactg agggtctctg
     1861 tgatatagga attttaaagg gcgatttgga agagctcatt aaaaatgaag cctataaacc
     1921 attttatatg cataattcag ggcattggtt aggattggat gtgcatgatg tcgggcgata
     1981 taaaatcaat aatgaatggc gtcctttaga ggctggaatg gtactaacag tagaaccagg
     2041 gctgtatatc agcgcaggga tggaaggcgt tgataaacga tggtgggata tcggtgtgcg
     2101 tatagaagat gatattctgg taaccagggc aggatatgag gtattgaccg gtgacttgcc
     2161 tgttaatgta gatgatattg aggctttgat gcgtggcgaa taaagagact gatatactga
     2221 ttattggcgg tggtttgact ggtgcaagct tatccctggc tctgcaggga ttaggtttct
     2281 ctgtcacgtt agtagaatcc aggccataca gcgataaggt aaatcctgat tttgatgctc
     2341 gctcattggc gttgtcacct gcaagtcaac gtatattggc aatgctggga gtttgggaga
     2401 ttctcaaaga gtatacaaca ccaattcata tgattcatgt ttctgatcag catcgctttg
     2461 gggcttctcg tttgcatggc gaaaaaaata gtcctttggg ctatgtggta gaaatgcaat
     2521 acatcaacag ggctttgagt attctaatca ataaagaaca cttgttagct ccagcggctt
     2581 taaaatccat cgattttaac aattctactg ccactgttgc tcatggcgac gatatgattt
     2641 ctatccaggc tcaattaatt gtcgcggccg atgggacgga atcgatgact cgccacctct
     2701 gtaatctgcc agtcaaaatt aaagattatg ctcaagacgc tattgtcgcc aatgtgggcc
     2761 tgataaaacc tcatggtaat cgtgcttttg agagatttac gcctaatggt cctcttgcat
     2821 tattgcctat gatgaatgac aaaatgtcat tggtatgggc atcaaagcct gaagaagcaa
     2881 aacggttagt gatgttgaat gatggtgatt ttttgcgaga actgcaaact gcttttgggt
     2941 ataggttagg acgatttggt aaagtaggaa agcgatactc ttatcccctg aaacaagttt
     3001 taatgcccca gcaaaccaaa tggcccgttg tttttgttgg taatgccgct cacactttgc
     3061 atccagttgc gggtcaaggg tttaacctgg gtttaagaga tattgcggct ttggcacaat
     3121 gcatcagcca aaagggatta aatccacaga tgttagaaca ttatttgaaa ttacgagcac
     3181 atgatcagca aatgattacc tggctaacag acggtttgat acatctattt acgagtcgtt
     3241 tacccggtat gggagtagcg cgtaaccttg gattgctggc acttgataat ataccggcat
     3301 taaaaaattt attagcacga tatgcgcgtg gttttggcgg aattactcct gatttggttt
     3361 gtgaaattgc attaagcact ggagaatttc aatgagccaa cattttgatg tacttgtcat
     3421 tggaggcggg attgttggct tgacagcagc tttagccatg gcccaacgcc attatactgt
     3481 tgctgttatc gatgcaggga cattgatggt caatacagac aagagtgatt tgcgtgttta
     3541 tgccatcaat aaggcatcaa aaatgttgtt aagtgaattg ggagtgtggc agcacctcga
     3601 tgatgctcgg gtcgctcctt ataataaaat gcacgtctgg gatggtgtga gtggcgcata
     3661 tattgacttt gattctcgca gtattgctga gccaaattta ggggcaatta ttgaagaatc
     3721 aatcttaaag caagctctgt tgcgtcgaat ctcatcagag tccaaaataa atttatttcc
     3781 tcaaagtgta atagatgagg tgtgccattt agagcggagc atcaagatct ccggccaaaa
     3841 tactgcctgg gaagggcagt tattgatgat agctgacgga gctaactctc ctatgcgtca
     3901 taagcttaaa gtggatgtaa ccagctggtc ttatgagcag caagccttgg tcgcaactgt
     3961 aaatagtgag aaatcacatc agaatactgc ttttcaagtg tttcatcctg atggcccttt
     4021 ggcttttttg ccattatcta atctcaatca atgttctatc gtttggtcaa ctcatgccga
     4081 taatgcgatg cgattgaagt tacttgatga taatgaattt agtcgggaat tgactaaggc
     4141 atttggaggt cagctagggc aaataaaggt agaaagcaat cgccatcaat ttcctttaca
     4201 aatgagacat gtcaagcaat atgcagggca tcgctggtta ttgcttggtg atgcagccca
     4261 cactattcat ccattagccg gtcttgggtt aaatgttggc ctggctgatg tgaaaagctg
     4321 gttaacctgt cttgaaatgg gtaaaggtga attgatatcc aaaaaagtat taggggcata
     4381 ccaaagggaa cgcaagcatt ccgtttggca aattattttg cttatggagg gatttaaaag
     4441 attatttgga acctccgcat ctcctgttac ctcattacgc agtatcggat tacgagcatg
     4501 caatgaattt accccgatta aacgattatt tatgcgccag gctagtggtt tataagtaat
     4561 tcttcgccaa aatattactt tttttcagaa aagatattgt gcttaatttt tagttaataa
     4621 ttcttatgta taattttttc gctaacctaa aaaactctcc gaggttttta aatcacttat
     4681 tcttatttct gaagtttttt aggttgccta ttattcttta gaggtttggt aatgaccaga
     4741 ttaaaatttt tttctaagtt tcaaaaagca ttacttcagt tggaaaccaa tgtctcaaat
     4801 ttgagtaatc ctctggtggg ctcgaaaatc aaggaattac agactaaaaa aatattggtt
     4861 cgagctgatg ggcgtagttt agatcatcta tataaattag gtggtttgcc acaacgtgtt
     4921 gaaagcgaag acctggaaaa ggttcgtatt accgatgtgg aaaaatacca gaaatttaat
     4981 gtgaatccat ttggatgggg tgcctgtgct tctgcagagg atttgcgaaa attcctggag
     5041 aattattccg aattgactaa acaagcatgg gttcataagt tttatggcag ttcaacttct
     5101 cttcttacct taaaatctga agtaggaata tcatgtggtg atcatgatga ggaaaaagaa
     5161 gaactgattg tcgatagcgt atcatttgaa caaatcatcg catctacctg cccaaaatat
     5221 agggaaaaat accttgatgg aggattggtc cccaatgcta tgaaggattt taagcccaaa
     5281 gtcccgcaca ccatgagact cggtgatttc agctcggaaa aaagcacttt ggaatggtta
     5341 ttgattaatg actgtgaaga agagtttgcc aagctttgca aagaaatata cggagatacc
     5401 cctcttaaaa ttttgttgat gcgattcgag ggggataaat atcttgcaga cgctgttact
     5461 tattatgtaa aggaatcttc atcatcccct agaatttaat gcttgataag gatcgtggcg
     5521 tagatttgat tgacaattca acactatgat ccttatttat ttgagcttct acatggtgag
     5581 atggatctaa aataagcgga ttcatatgat gtacttggtc aaagcgtata gagctattat
     5641 ttccaatcat tgattcaacg ctagcgtatc cccattcatt tccagttaag gcaaacgcat
     5701 cggaagggtt aataacggca ggcagatatt tttcaatttc ttcttcacga aactgcagaa
     5761 catcgcgtgt gctttgatac actttggtca agccattata gtcttcaaaa attgaatcaa
     5821 actgttcttg ttgaatttca ttaaaaatag gaaattcaat ctttgctatc catggaaact
     5881 tatgttcact tagcaatttt ttataagcac gtaaataata gggaaaaatc atatgcttta
     5941 tatcataacc gcaatcaatt tttgcaaaaa aacccattcc aactgcagtg gctttaagaa
     6001 aagccttttt tccagtttct tttgcggttt gatttactga tgccagtagt aatgagatat
     6061 cttctagctg atagcgaaaa taagcttcag ataggaagat aatagcgttt gtcttgctaa
     6121 agctgggata aaaaccacct gatgaaaacc ttgctagata aggaaaaccc aggcgaatac
     6181 gcgcaatcat cttgttggta tcatactcgc ccaactcttt tttcgcctca tcaaactgga
     6241 taggtaaacc atcatataaa tggctaaaga gtggtgaatc aattattttt tgtacagggt
     6301 ctaataaaaa taaactataa tggaggtaat tattttcaaa agaaggccct gctaaagaaa
     6361 aaacacacac ttttattggt tttcgatagg gtactaatgg gttggttaat gggtaacctc
     6421 cggaggcagt gaccaggctg tgcgaagaaa ctactttctt actgcctgtt aaccgttcta
     6481 taggagataa taattcagga tgtcctaaag ggattttctc gaaatatcct gcttcatcag
     6541 gaaaaggtaa gccgttttga aagaatccgc ttttatctcc tgacacatca atagcggcta
     6601 ttttaattaa cgcctcatta atcgtttcgg tgtcgggaag atatttctta ttagcaaaca
     6661 ctctaacgga cgattgcgtt tcactcaatt catctatctg aaatttttta tcgaggcctc
     6721 tgctttttaa ttgtgtttca aaccaaattt tagggggggt agctttcatc ggtcttgatg
     6781 cacgtttgaa aaatgaaaat aatttagacc gcataagaaa tctccctaac aaatttggta
     6841 tgccagcatg tcaaaatgac atttctctgt taataatttg cactaaaaat cgactttgaa
     6901 caaaattaaa ttcgctcgtt ttcatataca gaaaggagag gaattatatt tattgcacaa
     6961 ttaaaaccat ttgttgtttt attatatttc attgtgaatt aaattaaaac tcatcttttg
     7021 aaattgttag ctgatttatt tgctaaatgt atttgttttg taataatgaa acttgttgat
     7081 gatttgcatg gagagttcat gtttagacga ttatggttgc tccgccccgt aaacgcacca
     7141 gctcttgatt tatcaaggat ggctttgggg cgaatgcctg cagaaactac ttatgagtcc
     7201 ataaagaatg attttgagca ggaacttcat cgagttggac tgaagcaatt tcaagagaat
     7261 atccatgctg agcaattgat tttggagcaa ttattaaaac agcgggaaga gattacaaaa
     7321 aaaattgatg aaaaatgttt acggcctgca ttacagcgat ctgcgaacgg gtatgatgta
     7381 aaagggttat attggggcaa gattcaagag gatctgtcaa aaattcaatc taaaaaagag
     7441 cgtgtagaga tggatgaaat gatcgattgg ctgtgtgctc gtcgccgttt tttaaaggag
     7501 cgaaataatt tatctcttga taaagaacca gatcaccctg cttctctcct gctttagcgt
     7561 gcaaattctt tgcggcaaaa acttactttg tctaaagttg actcttgtcc gctctcttta
     7621 aaacgtgctt caatcgttct ataacgctta agcccaccct cgcggttggc tatttgtatg
     7681 tttaaccctg gtctggcatt taattccagc attaatggac catgttcttt atcaagtaca
     7741 atatcaactc caagataacc caacccagtt aactcatagc aactggaggc tatttcaaga
     7801 atctgattcc aatagggcac ttcgatacct acgatgggat ttagcgtgtc gggatggtag
     7861 tctatggtat cattttgaaa cacgccacca agagtttttc ctgttgcaag atcaacgcca
     7921 acgcctattg ctccttgatg taagttggct tttccaccgg ataaacgggt gggaagtctt
     7981 accatggcca ttgccggata gcctaataag gtaataatac gtatatcggg tatgccttcg
     8041 taactgacct ctgcaaatac cgggtcgacg accactcgtt tttcaatgat ggcataatcc
     8101 gaggatccgc caaggctata ggccccggat aacaggcaag ataaatggta actcagttcc
     8161 tgcacggtag tcagcttacc attaatttgt cgatatcggc cgtaaacttt atctttaaat
     8221 acgagtattc catcaccacc agaacctctg gccggtttta ccacgaaatc attatagctg
     8281 gcaaatcggg tttctattga tttgatttgt tgctcagttt caatgatgtc atataaagca
     8341 ggaactgcaa ttccagcttt taaggccagc tttttagttt tgagtttgtc atcaaccaaa
     8401 ggaaataact ttcttggatt gtatcgtaat acaaaatcag tattccgctg gttaatgctt
     8461 aaaaccccat gatttttcag gcgcctaaac aggttaatca tttcatcgca ctcgcaagtg
     8521 atttaaagcg ttttaattcg actaaacgat agccacgata ttgaccgaac cataaaatca
     8581 aggacaaaag caccaaaagt aattcgggaa aggcaaagat caaatattgt agcggttcat
     8641 aactcattgc tccgtatgaa ataactgctg cagcaagact accaattcct gattttattg
     8701 cttcggaagc ccctctttca tcccatgtga tacacatacg ctcgatagtc atggttaaga
     8761 tgaccatagg gaataatgcg acagacatac cggtgtcaag actaagattt tggcatagaa
     8821 cactgataaa gatcattaat aggatcacta cagtgagaat ggctgcaagt cttgggacca
     8881 acagtagtct taattgatcc agataaaatc gggctaataa tccgaaagaa ataataatga
     8941 caaataaagt aatgccccac gctacatgag tctctctaaa ggccagggca atcaagacgg
     9001 gcataaacgt accaaaagtt ttaatgccta taaaattacg cagtattaaa atgatgaatg
     9061 ctccaatagg aacagttaac aaaattttat agatcccttg tacattgacg ggtagctgga
     9121 gtaaggagaa gcgcaagagt tgcgagtctt ctagtcctct tgatttggcc acacttaagg
     9181 cattaatggg tgttggtgat acagttaatg aaaattgagc tctattaccc ccaacaacat
     9241 taaatagagg gccattacca tattgccaaa tcagaaactc ttttggcaag ccggcactgc
     9301 cagttttggg gttaatgtaa atccagtttt ttccattaaa aacagcgagc agggatttta
     9361 aatcggcctt tttttgttgg tttagataaa tcccctggac tgtaatggcc ggaatctttg
     9421 attgattgag aatcaaaatg gtggcattaa taatattgtc atcattgaat tcgttcccaa
     9481 ctaaaagctt cgcattacca tctcttttgt ttaactcttt aatagtgctt tgggcaaatg
     9541 tttgaatgtc tgctgatgtt gacctcactt gattggtaat cgtttcaacg gccgattttt
     9601 gactgtcatt cagaggttgt gatttaatta ccgaaggttt aggcaaagaa gactcatcac
     9661 tatccgtttg gcggaagatt gctctataat aaagtgattg aggaccatga ccacgcctta
     9721 tagaccatac tgtttctcta ttcgaaccgt ttaaattggt ggtgacacca taatttctgg
     9781 acacaaaata ttcatcgaga atggcaaaat tggggggcag gtaaggaata ttaaagcttg
     9841 cctttatcgg cgtatttcta tcagcggtaa aacgtaaatt ggattcaacc atccaactgt
     9901 tgatcgtttc agtatcggtc agagggacat cgagaatgat atgtctatat aaaaaaatac
     9961 ctgatcctaa aagcaataga gtgaggatca aaccataaac atgacgtttg ttatttttca
    10021 ttgttcactg ggctcttgga tttagttgtg aaagtaaggg cagggtcaac ggccccatta
    10081 aaatcaataa tggcatctct tcccaacaat aaagggtaaa ggaatcgttt cctgttggtt
    10141 aagttcactt taatcgttct gactttatca ccaagcttga tattaagtaa gactaccggt
    10201 cgcttaatgg gagttgttct taataatcct ggattggtct cacttgacct tactttaatc
    10261 tttactttgc cgacatactc tccttcaaat gagtaatcac cggttttggt tggaacggta
    10321 aaacgtaaat aaggaatgcc ttttttttca atttctgtga tattaacggc atgtaatgat
    10381 gctgatttag cgccagtatc aagctttgcg gataaagtga ggttttgatc gattaatgtg
    10441 gctttctcta cgtagccata gatttgagct tcactgtttg ccataagaga tcctgtcagt
    10501 agtgacatta acataaagag aacaaatttt gatctcattt aatcctcata ttgggtttta
    10561 ttttggtgca atatcgtagc aggtttaagg cataagtcac agtctgagag agaatttaat
    10621 gcaatttttt ttattttttt taataatttt gctaatagga tgtgttatta tgaagggttt
    10681 gaggttattg ttgtattttt ttaacccagg atgaaagttt aattgacatc aagtctaatt
    10741 atgcatacaa tagcgcccaa tcttagctga tagccccata cctatacatg cctcatgcga
    10801 gtgatgaaaa tcaggcttcc aagcctactg atttagtctt cattttagca attgctggtc
    10861 gtatcgacgc gcaaagtaga attctcgctc aaaaactcga taaaagtcgc agtatctatt
    10921 atgcctacgc tgttatggat tctctaagct cctcgtacag catgttcaaa tatttttttg
    10981 atctgtttgc atctcctaat gataacgatg cgatgcatga attcttaaat tctccaaccg
    11041 gtcttattgc cattgcagtt gagtcagtat ttttagttac cttttctgtt ttggcctgtc
    11101 aatttgagaa cgaaaaagaa gattactata aaaaatttat tgctaatgcc tggccatatt
    11161 ttcgtgatgt catgaagggc ttgaaaaatg cttacaaagg ctggagaagc gctgttgttg
    11221 cattcagttt aattggagga gtggatgcaa aatttcttat tcttccaatg ggagtgattt
    11281 tgggggtatt tgctgcagcc aacaggtttt ggatacgtag catgctggaa gatcgtaaaa
    11341 caatgatgag tgagaatata gcgcttctca tggagataaa aaaattaatt tctttatctc
    11401 atgaggaaag cttttcttat ttcaagcgaa tcagatacca gaccatgcaa actcgtaatt
    11461 tcgcattctg ggctgttgct gcaggtggac ttcttgatgg tttatatctc tacgttggtg
    11521 tattgggtct ggctgtattg tcaccgcagt tgcttatggc aatggctctt atttgtgcct
    11581 tttatacagt ggcttgtgtt attacccgta tttatgagga atatgatttc cagttgagat
    11641 tattagtgac acaaaccaaa tgtcaattgg ctttaattac caaagaactg gaaactaatt
    11701 acgccaaatt actcttattg caagacaaaa tggatctgga caaagttgat caggaagagc
    11761 tcaaacgact aaaaaggtta gtccataatt tgataacccg gtttgacgat agtcgtaagt
    11821 tattatccca acaatcgaac cgcacttatt tatcatctgc tttgttgggt atgaaaaatg
    11881 gattatatgc ttatggtgcg cttgcaagtg ttttattttt agtgggttct attttagtaa
    11941 tggcaggtgt ttcttttcct cctgccttgc ttacagcttg tattatttta ggtctggtat
    12001 tagtgctcag tttcactatt cattctttgg tgagtaatta ttggcatcag aagaagcagg
    12061 aaactcaaaa acaacgccca tatgagcgat tgatcgagtg gaataattgt cttaatgacg
    12121 aatttaataa gactgagctg ttggatccgg aagaatttca taaattacta aatgatggat
    12181 tgtctttaga cccttcaccg caatttttct ttcaagaatg gtttgaagtt ttacgttcct
    12241 tgttttccgg gtttggtaag gggcaaaaat ttattgactt tgttgggaat tcgttacagg
    12301 aactgggcga ggatggccat tatcgtgata cgcctgttat gtatgtctta tctgccttta
    12361 gtgccttatt gtttggggtt actctggcct taagagctct agccaaagga ttggggcgaa
    12421 ctcccttggc gcagcaggcc aagctgacgg aggacagcaa agaagcgtcg gttagccctg
    12481 agataaagaa ggaagttatg cagaaaaccg aagaacaaag ggaatcaatc caatctgaaa
    12541 aaactcggac tgattcccca gggcatacgg attctctgtc tttattcggt atatttgaaa
    12601 ccaaaaccaa aagaaatcaa cccgactctc catcaattcc tcattcgaaa agcgaatcag
    12661 cattaaatca attaacacct agtcagaaca gtacaattct gggtttagct taggatcgtt
    12721 atacatctta aattgatggt aaactctgaa cgtacgtgtt ttagctcgta cctcattaag
    12781 taattcatca agacacaaag ccaattgatt taattgttgc tgtatgattt ccagtttttg
    12841 tgtgcaggcc attctatgtt ctacgcctac atcctggcgt ttggtttgta aatccatatg
    12901 atatgatttc agtgataaaa tagataatct gtcaataatc atcccaggtg attcggaatt
    12961 gactgggcaa tgattatggt tttcaggttg taatgcttta taaagccact catccattgc
    13021 ttccatgcgg ttgtttcttt gctgattgaa agcgtctatt tctcttttgg cctggtaaac
    13081 aaattcaaag cccatgtcat ctcgacgcgc cttgtcttct gcatgccata attggaaatt
    13141 aaaagcatga ttttcttcta caagttgaag gaattgttta tgttgatact ctatgccatt
    13201 ggctttccag cgtatgatgc tcgttttatg aagggtagtg atagttgatg gatcaaaact
    13261 ttcttgcatg tcatatcctg cagattaatt tggattgtac tttaagatga gtttagtcta
    13321 tgaaaatata cctatttcca tatcctgagc tcgactgttt tgaactatct ctcatgcatt
    13381 gaggctcaat acattatgag agacaggttc aattggtatt agtttaacct gaattccgga
    13441 gttggatcta gtttcgggtt tttgaaatgc ctggtgaatt gctcaggata atatcctaat
    13501 cactgaaata ggtattatat atttccaaat aggttccgtc tttttctatt tgtaataaag
    13561 ccttgttgat tttcatgata agcggagcat tatttttgag ggctagtatt ccatagccat
    13621 tacctatagg aatgggatct cccacaaatt tgagctcagt cgcggaattg ctcatcgcgt
    13681 atttcgctgc attggcatta agcactactg cttcgatttc tcgatttgac aatgcgattg
    13741 ccatatcgga cagtttggaa aattgcatta aatcaacata atcctgaaaa tgctcctgga
    13801 ggaggccacc gtataaagtg tattttaaag taccaacttt tttacctttt agatccgtta
    13861 aggcattgat atggtcatta gctttcaggg ttacaatttg ggcattgctc ggcaaatagg
    13921 gaagactgaa gatgtaattg gtgccagcag tggatacgat tggagtaggt gccagcataa
    13981 tatcaacctt tcctgcgttg agattggtat atagctgttg aatgttcatg cctttgtatt
    14041 ggcattcttc cccaataatc ttgcaaatcg agttcatcaa atcgatgttg aatccgaaat
    14101 aaaactcacc tccaccagat cttgatacaa atggtggagc aaaatctgga acgccaataa
    14161 tgagtggttt tccaaaggca tggaaattta aaaagatcaa aagaatgaac atgaatcgtt
    14221 tcatgttttt aagtccattt aggatgtact taaaatttag gaggagcaat cgattttgtc
    14281 aaattaaagc caatgtgttt tttaattgat gtttttattt gctttttgca agccaacgat
    14341 ccagggcatt ggcaaaattg gttttttctc tcgcgcttaa tgcagagggg ccacccgtgc
    14401 gctctccagc tgaacgcaac tgttcattga tatctctaag agtcagacgc tgtttgatat
    14461 tatttgcagt aaatagttct cctcttgggc tcattgctaa cccttgctta actattactt
    14521 ctgctgccaa aggaatatct gctgtgatca ccaaatcttt agctttcata tgactcgtaa
    14581 tataatgatc tgctcggtca tagccttttt ctacaagaac tgagcgaata aacggtgaat
    14641 ttggataagt aaggtaggaa ttagctacca ggataaggtt ggtattggtt cgtatggccg
    14701 cgcgaaagag gatatctttt atcattttcg ggcaagcatc ggcatcaacc cagatcgtca
    14761 tttggtatcc taaagttttc aaatcaatag tttaagggta tagtaaaagc attatccttt
    14821 taaatcccta ttggggaaag gattatttta agatgttagg gaagtgtcgt aatgaagcag
    14881 ttaataagta taataaaagg acaaattcaa cagctcccta ttcccgacct gaaacatttg
    14941 ttgttaagtc accttaaaga agatgctcac gatttaacag aactcttttg ccaacaaatc
    15001 ccggctcaga aaaatcaatt ctcgcttaat cactcagtcc catttggttt accggcttcg
    15061 ctatctgtac tcgcaatcgt tttgacttat gcccccaatc tgcttccccg agaattatca
    15121 gagcagttgc tgacaacgtt agattcaata aaagagaccg aaaccagtgt tgataatcaa
    15181 ttagcagaga gatgcacgga gcaattgaat tattatagtg atgcaaaacc tgtccttatc
    15241 gcgttgaaag aaagaggagc caaggattta attcagatct tggagcaagc cgatgagcgc
    15301 agtttgttag ctttttctca tctggctaaa gataccgatt taaatgtttt aattgaaacc
    15361 tttttagtta aaaacaataa ggatttggat ttagtcaata gaaaccattt ggtacaattt
    15421 ttacgtcaac ctgatttggc aggatggtta acccgtttcc ttgattcatt aatcgatgaa
    15481 tctatcaatg ctctctcaat tcgattgttg ttgtctttta tttcttctga gaaagcgatt
    15541 gagaaactga aggaggctag agcctgcctt gctcagtatc ctttttcaat agatttgcac
    15601 gccatgcggg ctgtgtttga tttttatcag catgaattaa aaaaagattt attttctcct
    15661 gaaagccatt tagtcatgca gttcctgcat gatttattaa aaaacaccaa attagacaaa
    15721 caacaagagt tttttcagag tttaagccag gaaattttgc ttttgatcat tgagtattgt
    15781 atagcggaat ataacaaaac gactggggaa gagaagcaga actgtaaaaa cttgcttacc
    15841 aggttatgtt cggctttttc tgattccagc caagactttt tggggaaaat aagaacaaaa
    15901 attaatcagc tggatcctta tctctttgat acccctcagc tatatcagct ggcatccggt
    15961 ttaataacta aatcatgtaa ctcaggaaag gagcagttat ccagttctcc aatcgcttca
    16021 ttgttatcca tgccacaatt tgtcggagtt tgtacgccta aggttttaaa acaattagtt
    16081 gaaagataca gcttgtatac cttaactctg aatcagaagg actataagca agatctttgt
    16141 tttaaagaat atgctccata ttttgatgaa atcaatgaac atatggctca gattgagcgt
    16201 aatctgctta ttcatccgga agctcagtca ttttggttgg ataaaaaata cagcttattg
    16261 cagtttagag ttgagatcag taaaagtgct ttgctttatc aaatggagtg tttactggag
    16321 gcgaacaagg gaggagatga actaaataat aagggcttag aggtgttata tcattattac
    16381 gccttgaatt atcccaattt acgttcggat ttattcctta aagtggtaaa tcatatttat
    16441 tcacggtttt ttactaaggg gaataatgaa actgaagctc ggaacgggtt acttcactgg
    16501 ttaaaacaaa attttcctct aattgataag ttgcccaaag aattagacag aaaaaaagaa
    16561 atttttttat ataactctgc cgggaagaag attggatttc ttggtgagtc taatgttgca
    16621 atgacttttg ttaatgagga aattgtatta ttagctcgaa cgggctctat ggatatcaat
    16681 gagcctttat atgatcaaga gggtttggtt ctagggtatt taacggaaga gagttgttta
    16741 agaagcgaaa gtgccattca caaaaatgct gccgcctggg ttttagctgg tttgtccaaa
    16801 agtgatttgg aaagttcctc gtttggattg gagtctctgt tacacaatgt gttattcgag
    16861 aacgcgatag gggttttata tcaatgtgaa tccatttgcc ttgatgccaa taagcgtata
    16921 tggctgaata ataaattatt aaaagaaatc caagaggctc caggagaaat ccctgcatct
    16981 gttttatatc cattgctcga tcattttgag gatgaaacag tatttgaatt attaggcgtc
    17041 atcaggaata aaaacaatgc gcttgagctt tttcattata ttttaaaccg agactcaagg
    17101 cgttctattt tactgaatgg cttgtatgaa tctgattttc aaagattttt ggaacatcaa
    17161 ggcgctgatg tttgtttagc tgattatttg gttaattatt atgataaacc ctggttttca
    17221 gaagtcttac tacgatttgc ttaccatggt aaaaaatata aaaagccaga tcttctaagt
    17281 agcgccttga ctttcattac caaggaaatt tctcaagaga agaagcaaga gacaatctgt
    17341 gatgctgtac tggatcgttt aataaattct gaagcatgtg ccgtattagt gttaaaggag
    17401 tttcttggtg atagaactca taactccatt cagaaggtaa ataatccgga aattggcaaa
    17461 gtaagccaat tttttgatag gcggcatata gtgcccctta ttgcacagtt aaataagact
    17521 tcttattggg agcaaaatgc gcagtataaa ttggctttac atattttggc taatcaacat
    17581 ggacgccttt tttctgaaac tgatttgaat cccgattctc ctcaggcttg gcaaggtaaa
    17641 gaattaaaag aactggcctg ttttacaagc aggcatttat ctaaaaaacg tcctttcgat
    17701 aataaacatg atattggata tcgtgtttta ggcgagcttc ttttcaggtg cgccaattcg
    17761 gggataacgt ctttatttta caagcaaaaa acgttgaatc gcgcaataac ccgattgtca
    17821 attacagaac cctttttagt caggatggtg gataaattat ggatgcctaa aggaatcagg
    17881 gaactttatg gaaagtcttt gttttttaag ggttcctggt ttgatgatca agtcttcttg
    17941 caaaaggaat taaaagacca tcatcctctg ctggactgga gtcattttat taagcaaacc
    18001 tggagtaaaa gagataaaca gaagaagtca cccataatat gttcttattt gctaagttat
    18061 acaggtaaga aaagcgtatt gtcgcattta ttacaggatt attttaattc ttttcaagga
    18121 atgccagact acatcaggcc agtcagtcaa ttattgagat ttttccctca acgcgatgtg
    18181 tccgcagtca tatacgatgc gctggaagct gaaatgataa aaaaacctca gttattagac
    18241 aggcaaattt taagtgacat ggccttttat tatggaaaac agttggccag gaaggacaca
    18301 agtttccccc aggcggatat taatttatta acgtattggg ggcagaataa acattattct
    18361 ttagtaaaaa gaggatgtga actattagtc agggattgtg aggataaagg agtaaaaaaa
    18421 cgattggtta gtgctgtcaa agaagcagaa gtagaacagg acttgagttc cagtgtgcaa
    18481 acattttatt ttgggctaat taaatttttt aaaagattat ggcattatgg ttttaatgct
    18541 gaaaacagct catcaaaaat tataaagttt tgtgatgatc ccactcccgg ccctgctcgt
    18601 aaaaaagcag gggatctcat caatgcaccg gttgcagaaa gcagaaccaa ttctttgtat
    18661 ttaggctttt ctgaaagacg caagcagtta attagtcttt tagctactat taaaagtagt
    18721 ccggcgcatt tcacacgagt tgtcaattat tcagacaata ctcaaacatt ctttggggaa
    18781 caacccaaaa agtccattaa ctataatact cctgcaatac caagtaactt tgaaagtata
    18841 atgtaggatt gtccttatag ggcatttata ccttcgctca tatttttgtt ttattagcca
    18901 ggcgttctga atgcattaat caacccgatg catgacttat caatggaagt cttatatgag
    18961 tagggatatt atatgagtgt tttcttaaat cgatgccgat attaatgatg ggtttattag
    19021 tgaagattta ataatttttg gttacataaa cactatgggg tgaatcatga gaattttgct
    19081 ctctttctat cttttattgc tttcaacagg gctttttgca gaatctactt ttcctgatgg
    19141 ttgtcgggct gttcctgtac aaggtcaatc agtgacttta aaagccaaga aaaataagtt
    19201 ggtgtttatt cacaatatta ccaacttgga tctatggatt actcatccgg ttacagatcc
    19261 tggggccagt gcgggttgga caagccgatt gcaagctggt aaatggtcag ctttggtagt
    19321 ggacaaacct gcctttattt taaattgcat agaatcaagg ccagggcatg agcaacagat
    19381 accttgtgaa aatgctattg ccgtatgtca atggaaagga gtaaaaatac cggcaagcaa
    19441 tcaaggtact ttctgggtag cggaagacct gtcattagca gcattaaatg ccgcagtcgg
    19501 tggaagaggc tttgtattgc cggagaaata atttatcaat gaatatcttg gggcatggct
    19561 atggactcta tgctgcaaaa agtcatatca attctcatta caattcaaga tagccactga
    19621 tgaggctatc ttgagccttt ggcaaattgt ttagccagct gccatgctac ccactcttga
    19681 gtccttatga aaacttaaaa ttttaataat gggctaaatg gattgagatt atgcaatata
    19741 aaaaagcctc atattattat gaggacttct tgccctgtaa gattggaaaa aagatgaata
    19801 aaaggcgttt tatacagaat tctctgatag ttggcagcat attcttgcta gttatagtga
    19861 taaatggtag aacaggactt aagaggtttt agttagcatt aggtttgaag agaaaaatgg
    19921 tgaaaagtga gtaaaaaatc caaagatccc ttttataaga gggaacgcga aaaatacgct
    19981 gagccaattc caagccggga atacatcatg gaaatcctgg atgaatacgg taggccaatg
    20041 tctcgcagta aactgtttga taaattggat ttgagctctg agagccagca ggagtccttg
    20101 ggttttcgct tgaaggctat gctgcgtgat ggccaaatca tgcaggacag gcgtggacgc
    20161 ttttgtttat tacaaaaaat taatttattg cgtggaacgg ttcaaggcca tcctgatggt
    20221 tttggatttt tcatcccgga tgatggttct gaggatatgg tgctctctgc taaagaaatg
    20281 agggctgtca tgcatggcga tgtggtgctt gcctatcaat ctggattgga tagaagagga
    20341 agaccggaag ctaaaatcca cgaagtcctg gaacatgcga ataccacggt tgtaggtcga
    20401 ttttttactg atcatggcgt gaattttgtc attcctgata gcaaacgtct gactcaggat
    20461 atttcaattc cccaagaatt cattggcgag gccaaaaatg gccaaatagt actggttgag
    20521 ttaatcgcat atcctaacaa gcgttcgcaa gctataggca gagtaatcca tattttgggt
    20581 gagcatatgg ctcctggcat ggaaattcag gtggcaatcc atgcccatgg cattcctttc
    20641 gaatggccag atgatgttct tgcggaagtg ggtaaaattc cacaacaaat ttccgaggaa
    20701 caattaaagg gtcgaaccga tttgcgtaat cttccttttg taaccattga cggggaagat
    20761 gcaaaagatt ttgatgatgc tgtgtattgt tacaaaaagc ccaaaggcgg cttccaattg
    20821 tatgtcgcta tcgcagacgt cagtcattat gtggcgaagg attctgcttt ggatcaagaa
    20881 gcagcaaggc gaggaaattc agtttatttt cccggtaaag tggtgccaat gctaccagaa
    20941 gctttatcca atggcgtttg ctcattaaat ccccatgttg atcgcttatg tatggtggct
    21001 gagttatcga ttacccaaga aggaaaaata tcccgatccc gcttttatcg ggcagtgatt
    21061 cattctcatg ctcgtttgac ctacacgtca gttggggcat ggttggaaca aggcaaggca
    21121 gatgaaaaac atcaagcctt gtggcctatg cttgagtctc tttatgattt atatcatgtt
    21181 ctgctcgata cccgtagact tagaggtgct atggatttcg agactacgga aactcgtatc
    21241 gaatttgatg aaaataagaa aataaaatgt atcgtgcctg taatacgtaa tgacgctcat
    21301 aaattaattg aggaatgcat gctggcagcg aatgttgcaa cagcaagatt tttagaaaag
    21361 gctgaaattc ctactttata ccgagttcat gcggctcctg aagaggataa aattactgca
    21421 ttgaggcagt ttttagggga gctgggattg tcattgggag ggggtagaaa acctggaccc
    21481 aaggattttc agcgtaccat gaacatgatt ggtgaccgac cagagaagca tcttattgaa
    21541 acggtaatgc tgcgttcttt aaaacaggct cagtacgttg aatcgaatga aggtcatttt
    21601 ggtttggcct attccgctta cacgcatttt acatcaccaa tcagaagatt tcctgatcta
    21661 ttaattcaca gagcaatagg gcatttactg gataatcatc ctgtttatga atttgagtat
    21721 agccatgaag atatgaatcg tttgggtaaa cattcctcga tgacggagcg ccgtgctgat
    21781 gaagcgaccc gggaagtgat tacctggcta aaatgcgagt atatgcaaga caaattgggg
    21841 caggtattta agggtcgaat atccgctgtg accagctttg gtatttttgt ggagcttgat
    21901 gaaatttatg tggaaggctt ggttcacgtc acttctttaa aaaacgatta ttatacgttt
    21961 gactctgcaa aacatcgttt aataggggct cgcggcggtc aagtttatcg tttgggagat
    22021 aaaatgactg tgctggtagc tcgtgttgat ttaaacgaac gtaaaataga ttttgagcct
    22081 gtcgaggatg aagtttgtaa tgaatgagca gtatgtttac gggttgcatg cagtttctgc
    22141 attattagca aacccgcacc gaatgatcaa aaaactcttt gttaatcaag atagaataga
    22201 taaaagactt catgagtttt tggtcaaggc agaaaacgcc catgtcccca tcgaaaagct
    22261 aagctcgcaa aaaatgaatc agcagttttc agagtttacg catcagggcg ttgttgcact
    22321 cgcttcggca ttacccgaat acggtgaaag tgatttggtt actttattag atagaagtaa
    22381 aaagcctgtg ttgattctta ttctggatgg aattacagat ccccataatt taggcgcttg
    22441 cttgcggtcg gctgatgcaa ccggtgttga ttttgttgtg attcctaaag ataaaagtgc
    22501 gaatattact cccgtggtca gtaaagtggc ttgcggcgct gctgaatcaa ttcctttggt
    22561 gagagtaact aatttagcaa gagctatgga catcttaaaa caacagggtg tctggatctt
    22621 tggagcagct ggcgaagcag agcatttact ttatgcaatg gattgttctg gaaacatcgc
    22681 tcttgtaatg ggagcagaag gaaccgggtt aagaagattg actcgcgagc attgcgatgg
    22741 attgttttct ttgcctatga tgggaagtgt agagagttta aatgtgtcag tggcaactgg
    22801 tgtttcattg tatgaagtaa ttaggcaaag agggcaattt cgtgagtgaa ttaaaaatca
    22861 aagcagccaa agcagctata gcttatattg aagatgatat ggttataggt gttggaacag
    22921 gctctacagt taatttcttt ataaaagaac tggcagcaat caaacataag atagaggctt
    22981 gtgtagccag ttcaaaagca accgaggcat tgcttcgggc agaaggtatc ccagtaattg
    23041 atttaaattc agtacaggat ttacctatct atgttgatgg cgctgatgaa gtcaatgaac
    23101 gcggtgaaat gataaagggg ggcggaggag ctctgactcg ggagaaaatt gtagcgaatg
    23161 tagccacaca atttatttgt attgtcgatg aatccaaggt tgtgaaacgt ttaggggaat
    23221 ttcccgtggc tgtggaagtc attccaatgg cccgtagttt tgttgcacga caaattgtta
    23281 aattaggggg agaccctgaa tacagagaag gtttcgttac tgataatggt aatattattc
    23341 tggatgtgtt taatctgaat ttctcaacac ctatggcctt ggaagacagt cttaatgtaa
    23401 tcccgggtgt cgttgaaaat ggagtgtttg ccaaacgttt ggcagataaa gtgttagtgg
    23461 cttcagcaag tggtgtgaat aatttaaaat agtcgacttg taaacgttca tatctcaaaa
    23521 cattaattgc caataatttt ataggttcct caaaaatgga tacgcacaag gtttttgtaa
    23581 atcgaattat aaatatgaga aaaattaaac tgattggttt ggatatggac cataccctca
    23641 tccgatataa ttcgaaaaat tttgaatctt tagtttacga tctcgtcaag gaaaggctgg
    23701 cagaaagctt tcactatcct gaggaaatta aaaaattcaa atttaatttt gatgatgcta
    23761 ttcgtggttt ggtgattgac agcaagaatg gcaacatttt aaaactaagc cggtatggtg
    23821 ctattagatt gagttaccat ggtacgaaac agatcagttt tagcgatcag aaaaaaattt
    23881 acagaagtat ttatgttgac ctgggtgatc cgaattacat ggcgattgat acttcttttt
    23941 ctatagcatt ttgcatcctg tatggccagc tggttgattt aaaagatacc aatcccgata
    24001 aaatgccaag ctatcaagcc atagcacaag acgtgcaata ttgtgtggat aaagttcac
//
LOCUS       DASLOE010000030        24038 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.030, whole genome shotgun sequence.
ACCESSION   DASLOE010000030 DASLOE010000000
VERSION     DASLOE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 24038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24038
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.030"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            298..2910
                     /locus_tag="U6309_000981"
     CDS             298..2910
                     /locus_tag="U6309_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1449 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504434.1"
                     /translation="MKEKMILDKSNIINAIFSNLSADEIRKVKGVSHAFYDRATEYLT
                     GHLENENFHLNRVIPPSDAIQREQKIEFCAKFFQTLIRSISNGEEGSSGQLYKFNNMM
                     EKIRRAVQSNLGLSRVQRETYETFHPIYVKLGAPNFPINDEGKLDPGSIVYRTDHVKG
                     RDERKSSWLVLLKDDSNEDGLSICFDTKFRKYLLHIDGAGHVTSQLTLRWDAIYDDLL
                     PETSMQQPLRSFTPWQRHAEANFFNGRSTNKILGLLTKNYPEIFNYDPTSYFKEEPVF
                     SVELNRNAPKKLSTDKKNIYLTLDESFFKTPNGFENGLNRQPILAVIKTNSPLRGDCF
                     SVVPAIYEKQKLHILIKGHQQQTQVVDVFHANFKTVELLSWELLDDVIIFGEPSTEYK
                     PALTDLNLLSDQQSELMSHVSTAPMVRGSGPGARQYWLNGFFKSTVYSEMIVIAEIFN
                     RRFMSTPLASLEQTLNHSDATKSFYRLQFSHQELEKAKEFVFQLAIPNEFSKQEDFDF
                     FYGGDGNKLEEITKLAILMLKRMVPKEFHHKIRIGINMCCPAASVENIGSGFGLRNKP
                     DFIERIVKAIKDADSNLKVEFKTLMLSHEDVYGDGDQISGNTKNISEIQNERLTRIVY
                     KTKANRLVYQGKPRNIEAYNVDAVSCIIREVNTPKEFQFGYSGMVATITNHDINQLGY
                     KVVGEPHYSVQSILEKIKKQLGEVPIEFEVMLGRVLLGSAWVLQGRYASDAEILLHTL
                     LQAAIEQEWARGMGRYGIDIYQIQVLYNIRHITNDNYRSSLINKILNAQSTEEIIDKI
                     FEQYFSIKKKGECNILQLQRLEKAVFAAIPNSKALQQYKLITEPNSKSRTGFQFFSNQ
                     DKKQTLEEKSIFTP"
     gene            3006..3989
                     /locus_tag="U6309_000982"
     CDS             3006..3989
                     /locus_tag="U6309_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopenicillin N synthase family oxygenase"
                     /protein_id="HEN5504435.1"
                     /translation="MKNKNKSVITVDLNDPVKSLEYLEEAFQIGTFILKNSPVNFKRL
                     AKVLSDLDTFYNLDFKTKIEISGQISGGGATRGYFPLGIESGLVDVFEKKESYSFGWP
                     DNEIPDSDTPNLSLVAPNIYPEHGYINQDDVGGLIKDFANTAKELSKLIEIILFNGKA
                     ALPIDESSDFQSLLRSFRYYKKTELDPPNTGANSLHTDWNLITLVYADADGYQYRDNE
                     GNFQNIIPETDDCLIVNFGDFLSAWTGGKIVSPWHQVVEAKKKDVRNSLVFFYYPAAS
                     SSAVKNPIYNQGEPLSLFVDQSDPLNRLWDFSKIETMNEMYAEKWKQVSRK"
     gene            complement(4115..4564)
                     /locus_tag="U6309_000983"
     CDS             complement(4115..4564)
                     /locus_tag="U6309_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEN5504436.1"
                     /translation="MSLLLTSPAFEHNDFIPDEFTCHGLDCPPPLAWENAPPNTKSFV
                     IIMDDPDALGGLWVHWVLFNIPASYNELDAAIDLPDSAISVKNSWGKTGYGGPCPPRG
                     THRYFFRLYALDTFLSLDEKATKKDVEYAMQGHILESSELICKYTKL"
     gene            4782..5147
                     /locus_tag="U6309_000984"
     CDS             4782..5147
                     /locus_tag="U6309_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504437.1"
                     /translation="MKDLLVYVLCFIFSSLINVAWADNNLCIAINNFGTVPINVSGAN
                     SIEEHTVKPRETLILPEKEVSAACFPDWGCDVFILPIEQNAKGLYLKQLPKGSRIIYS
                     QPDLYYVNRTANVPCIPDL"
     gene            5186..6037
                     /locus_tag="U6309_000985"
     CDS             5186..6037
                     /locus_tag="U6309_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN5504438.1"
                     /translation="MTSYKEKKVKIPGFTIALKIWNSENPNPVLCLHGKMDNAASFDL
                     LAPLLSDRQLVAVDYPGTGLSSHYPEGVVPHWKNDAFLMCHVIKALGWKSFDIIAHSL
                     GSFLANVITIAQPKLVKKLVFLDILGPTVHIIENSMAYLPLNIESYLISGKQPRTVFS
                     SLDAAIKDRMNIGNISYQAAQALVQRGTKKDNEGWVWTFDPRLRCISATIPHEDEIRA
                     MFRAIDVPVCLILANQGVSYPESVFIGRSQSIKNLTIHRVQGGHHVHMDDPASVAEII
                     SQYLSSK"
     gene            6164..6682
                     /locus_tag="U6309_000986"
     CDS             6164..6682
                     /locus_tag="U6309_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1453 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504439.1"
                     /translation="MPRWFRRIVDEGERLLVEVTDELRDESEVHKAASQLHLRVEQVL
                     NANRARLEKPVIEAAEGFLHDLGVILEEDSMLRFPHAPVNQYLLAEKCTDIIYNYKPS
                     LESAPGIWNQIKAHINNFIEIIFGLENVLPTTQTMFAKDVSFTRCKRNLSRLKESLES
                     DAPDLLSVLVNG"
     gene            complement(6807..8192)
                     /locus_tag="U6309_000987"
     CDS             complement(6807..8192)
                     /locus_tag="U6309_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5504440.1"
                     /translation="MKKNIIMFIVSCAMFMEAIDTTILNTAIPVMAHSLEVNPIDLKL
                     ALISYLLSLAIFIPISGWIADKYGVKKVFIAAIGVFTLSSLWCGFTQSLWELVLARII
                     QGVGGSLTVPVGRLIILRTCERHELITKMSIVVMIASLGMLLGPLLGGIITDYFSWRW
                     IFWVNIPVGIIAMLLSHKLLPTILPRSVPPLDKSGFIMFGAGLAFLTLGLSLFSETKA
                     DIVYSASITLLSALLLIGYTKHSRSKRNPIIKVTLLNIRTFRIAVFGNLLTRLSFGGI
                     PFLLPLLFQIILEYSPQLSGMLLTPVALGVFLVKPLSFAILRRFGYKKLLLLNTVLVC
                     FSLWSFATINQSTSVIYIGFLTFLYGFFIALQYTGMNSLAYANIDDNDMSYATSIMST
                     VQQLSQSFGVAISALLVSLFTFQVSQHFVLTVKIFHLTFFVLGILTILSGLIFTSLKK
                     EDGKELIESPT"
     gene            complement(8221..9477)
                     /locus_tag="U6309_000988"
     CDS             complement(8221..9477)
                     /locus_tag="U6309_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEN5504441.1"
                     /translation="MLKNITRALSLSLLISHNICANEASSIETISQPTTIQFLEKFGA
                     GFANLEHKNLGDRVQLYLPGHNSKTGIVKLRLPNGSKLSYGEIVMYGGDMFGNPAKPI
                     SNCSEKNRLACFKAQFDALGTLGNKQDKRCSNPLNQITHLDRFFTNLAEEIEKSKQNG
                     TNDWDYYKQHDSAISKELNKLTCGGSIISGFIPFGNYIKLAEVNYDHFVPDSLVAYKT
                     GHRFALETALKAYEKKQQGLIDEANKLLELAYAQNAFANHYLTDSFSAGHMRTPRRAI
                     GHDILLPSVLNLLIANLMHNEDNQHGLNVVNHEGISWLAYGDGYLYKPEAELQRVIIL
                     DAMQRSADSIHSTFMNGVIPEHYPEMDVFPDYSKIEQINDTAPLFKVENGVLLKRVNN
                     KDHYDHHWTKYWSGLVTLIEFQVNKI"
     gene            9598..10932
                     /gene="rlmD"
                     /locus_tag="U6309_000989"
     CDS             9598..10932
                     /gene="rlmD"
                     /locus_tag="U6309_000989"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN5504442.1"
                     /translation="MRKVKPKLNLTSQTARIVNLSHDGRGIARVNGKATFIQGALPGE
                     VVEFQYTRVKKDFDEGKLLSIVEPSTLRVEPKCPHYQMCGGCSLQHMSAEEQIRFKQS
                     HLLDLLSRYGHTEPQSVLSPLTSHHWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFI
                     TEINQCPILNSKIDTDIVHLRKLIDTMEDKQCIAQIEVAAGDNEVALIFRNLSPLTEQ
                     DELKIREFAQQFQYKVFLQPGGLDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQ
                     VNAELNRKMVTQAIQLMELKNSDIVLDLFCGLGNFSLPMAKHCSRVIGVEGNKNMVER
                     AYMNAKSNHITNVDFYAANLDDVMEVRNLVNTSFSKVLIDPPRSGALEIVKQIDSIDP
                     ERIVYVSCNPITLARDTDILVNQKGYVLITAGVMDMFPHTAHVESIALFQKG"
     gene            10938..13142
                     /gene="relA"
                     /locus_tag="U6309_000990"
     CDS             10938..13142
                     /gene="relA"
                     /locus_tag="U6309_000990"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HEN5504443.1"
                     /translation="MVRVKDTTPLTPDGSIDVEMWLHHLGSKGYLDNLELVRAACTLS
                     QLAGQDHATETGQTCLQQGLSMADLLADLEVDQETLAAAIIFENVHYADLSIDDVEEQ
                     LGHNIAKLVKGIEKMSAMNNFQALNKYPQNKNQIDNIRKMLLAMVDDVRVVLIKLAEC
                     LCILRTAGHLSETVRKQLATEAMEIYAPLANRLGIGAIKWEMEDLAFRHLHPEEYKAI
                     AKGLKAKRLERDSFVNRIVEQLEHQIRATGARHFAVYGRSKHIHSIYKKMKRKNVSLD
                     EIYDATAVRILVDTEAQCYEVLGMVHTLWKQIPAEFDDYIINPKSNGYQSLHTAVEGP
                     EGRVFEVQIRTFHMHDLAEMGVAAHWKYKEGAVSQKESHERKIEWLRDVLAWHQEMAA
                     NKGVAENVTTEFLEDRVYVFTPDGDVLDLPHGVTPLDFAYHVHSNLGHRCRGAKVNGH
                     IVPLTYQLKTGDRVEVLTGKEIKPSRDWINPHLNYLKTARAKAKVLHWFKMQDYEDNV
                     RDGRELLEKELKSLGIKSDKLNEVITALNYKKADDLYASLGRGDIKIGQIVNRLAPPD
                     TSEQNLQKFVRQQQKPEVTGSDLRIEGVGNLLTFMARCCQPVPGDEIVGYITIGRGVS
                     VHRKDCPNIIHASEKQKRRFIQVNWGTATRENYVVDVLIKAFDRSELLKDVTSLLSNE
                     KAHVYALQTQSNKHENMAYITLTVEIDGLNSLSRLLTKLEQIPNVLEARRQL"
     gene            complement(13182..13547)
                     /locus_tag="U6309_000991"
     CDS             complement(13182..13547)
                     /locus_tag="U6309_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEN5504444.1"
                     /translation="MNINLETAEQHAVQAYSDKKIQINSIIYESSLIVSKKEIITDFV
                     IKDIQDINNSYIDLLLRSKPELIIIGHLQTGKTLPVTLISQLSQKRIGIECMSIGAAC
                     RTYNVLLSEHRAVVAGFIF"
     gene            13606..14799
                     /gene="alaC"
                     /locus_tag="U6309_000992"
     CDS             13606..14799
                     /gene="alaC"
                     /locus_tag="U6309_000992"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEN5504445.1"
                     /translation="MSQFPRIKRLPPYVFNTINQLKMEARARGEDIIDFGMGNPDQPT
                     PPHIVNKLIEVAQRPDTHRYSMSKGIPRLRRAMATWYLRHYDVHLDSETQILATIGSK
                     EGLAHLALAISGPGDTVLVPDPAYPIHTYGFIIAGANVKQIPLIDEVQFLAAVEDAIN
                     RTWPKPKALVLNFPANPSTHCVEYEFFEKVVDLAKRNNIWIIHDLAYADIVFDGYKAP
                     SILQVPGAIDIAIETYSMSKSYNMPGWRVGFACGNEELVAALTRIKSYLDYGTFTPIQ
                     VAAITALEGPDDCVLEIRNLYEKRRNLLCDGLNDIGWEVSKPKGTMFVWAPIPLNYRA
                     MGSLEFSKYLLKEAQVAVSPGIGFGQQGDGYVRFGLIENKDRIRQALRNLKALFKRDG
                     LLKAI"
     gene            14804..15613
                     /locus_tag="U6309_000993"
     CDS             14804..15613
                     /locus_tag="U6309_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504446.1"
                     /translation="MNVIINTESDCDSKEAKLLKSQGLAVINLLACGGYDLVNPPVGN
                     LLKSSHNLEGDWVVLTPMHWQASHNDAVIVALDKDLRVTDEEVKYWFDLYSAFLAEEG
                     MSLYFYDKYTWLLRVDDKPPLNAKPIYQVLNKSLMPELSQLDETMYWQKFFTESQMFF
                     SSNAQKSLINGVWAWGSGKLKTKKTISICTDKHFLAVAQAYSSNVTLYDPLVNVSGFE
                     VVLLHDINSLSELHQVEIKKIPAHWYWNNCVLIKAKSHWFTRLWRSLTHAD"
     gene            15603..17342
                     /gene="recJ"
                     /locus_tag="U6309_000994"
     CDS             15603..17342
                     /gene="recJ"
                     /locus_tag="U6309_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEN5504447.1"
                     /translation="MLIKQRQQPEFTVNLPNVPDVLKRIYITRGISDESQLDKQLQTL
                     LPFHTLKGINEACLRLENALRSEQRILIIGDFDADGATSTALAITALRAMGARFVEYL
                     VPNRFEFGYGLTPAIVEVASKWQPHLIITVDNGIASFEGVDAANKMGIDVLITDHHLP
                     AESLPDACAIVNPNQPGCLFPSKSIAGVGVIFYVMLALRRHLSNCNWFNEINMPEPNM
                     ASFLDLVALGTVADVVGLDQNNRIMVNQGMMRIRQGQCREGIKALIEIAGRDCSRLRE
                     SDLGFAIAPRLNAAGRLDDMSLGIECLITTDPKMARNFCQQLDELNLERRQIESEMKE
                     QAMLALEKLSINDEENKHLPVALCLHDKTWHQGVIGILAGRMKEKYHRPVIAFASVSE
                     HELKGSARSVPDLNIRDVLAAVDKDNPGLILKFGGHAMAAGLSISPELLNDFRSAFVS
                     EVLKHLDLSQCEGEILTDGPLQSHELSLETAQLIQQAGPWGQAFAEPVFDNVFEILDQ
                     RIVGKNHLKMTLISSQGGEPLDAIAFNVDLKSWPNHRIKYIHAAYKLDINFYQGRTRL
                     QLLIQAMNPVQQA"
     gene            17423..18415
                     /gene="dusA"
                     /locus_tag="U6309_000995"
     CDS             17423..18415
                     /gene="dusA"
                     /locus_tag="U6309_000995"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEN5504448.1"
                     /translation="MVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGA
                     VQNNPDRALYFNSVEHPIAIQLGGADKSALVKCAIIAEQKGFNEINLNLGCPSDKVQA
                     GRFGACLMKEPEHVADCIRAIKQVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLVEAGC
                     DKLIIHARKAWLNGLNPKQNRTIPPVNYDYVYELKKEIPHIFITINGNILTIEEINSH
                     LKHVDGVMLGRLACDNPYQIAEIHQALYPEFTKIKRSQLLMNYLDYWFDQSRANLSLS
                     ILVKPLFNLAFGLPGAKHWKKKLMNIIQEKNTSGFSELIQYLLNLEAINQVEFV"
     gene            18516..21206
                     /gene="secA"
                     /locus_tag="U6309_000996"
     CDS             18516..21206
                     /gene="secA"
                     /locus_tag="U6309_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEN5504449.1"
                     /translation="MLSTLIKKMFGSRNERTLRRMEKSVMAINAFEPKMQALSNEELA
                     GKTQEFKERFNNGESLDELLAEAFATVREVSLRTLGLRHFDVQLIGGMVLHEGNIAEM
                     RTGEGKTLVATLPAYLNAISGRGVHIVTVNDYLAKRDSQWMKPIYEFLGLTVGVIYPD
                     MSHKEKQEAYKADIVYGTNNEYGFDYLRDNMAFSLTDKVQRELNFAIVDEVDSILIDE
                     ARTPLIISGAAEDSSELYIKINSLIPQLKKQEEEGDEGDYTVDEKQKQAHLTDAGHLH
                     IEELLTKAKLLDPGESLYHASNIMLMHHVNAALKAHAMFHRDIDYIVKDNQVVIVDEH
                     TGRTMPGRRWSEGLHQAVEAKEGVPIQNENQTLASITFQNFFRMYNKLSGMTGTADTE
                     AYEFQQIYNLEVVVIPTNRSMIRKDEADLVYLTQADKFQAIIEDVRECGVRRQPVLVG
                     TVSIEASEFLSQLLKKENIKHQVLNAKFHEKEAQIIAEAGRPGAVTIATNMAGRGTDI
                     VLGGSLAADLANLPADASEQEKEAVKKEWQKRHDEVIAAGGLRIIGSERHESRRIDNQ
                     LRGRSGRQGDPGSSRFYLSLEDNLMRIFASERVASMMRRLGMQPGEPIEHSLVTRAIE
                     NAQRKLEGHHFDVRKQLLDYDNVANDQRQVIYTQRSSIMAMTDTQEVVEMMREEVIDS
                     LVDTYIPPQSLEDQWDPQALSDVLSDEFKIKASVPDWIDKDHSIQPEQIKEKILALAI
                     EHYDEKVRKVGRPVISQFEKSIILQTLDNHWREHLAAMDQLRQGIHLRGYAQKDPKQE
                     YKKEAFSLFTMMLDNLKYEVIRILSSVEIQTEEDAQVVEEQRRADQIRKMNLMHESLS
                     ENDEASETQTFRRQEKKIGRNDPCPCGSGKKYKACHGSLV"
     gene            21203..21607
                     /gene="mutT"
                     /locus_tag="U6309_000997"
     CDS             21203..21607
                     /gene="mutT"
                     /locus_tag="U6309_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEN5504450.1"
                     /translation="MKVAVAIIIDEKQRILITQRPFHVAHGGFWEFPGGKLEPHESPE
                     DALVREIREELGIIVNEYRFLGYVDYDYPDKHIQLIIFMVTRFTGNPLCQEGQLNMKW
                     VKKEELNINDFPKANHAVFDLIDIPELQYQAL"
     gene            21836..22654
                     /locus_tag="U6309_000998"
     CDS             21836..22654
                     /locus_tag="U6309_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEN5504451.1"
                     /translation="MITKQNGIYTLTLSLLLFSLQIKAATDYQTYETVCGEPTDMLSF
                     LERGGIATNPCVVPPKSVLISSGYQYQQLIGEGVQHNFPAAAIQLGLPGYFEVDLLLP
                     NYINQTVDPRIGFSETQLIVNHVLWFNNKWVITASGTFVFPSGSASFGSPNPGGGVIG
                     ILSYNFNSQLNLTGNLGITSQSEPSYDGGQSYTSVNPDLILSWTKNKISLFAEIYGQS
                     KTAPDEGSGFSTDAGVLYQVKKNIAIDFEVDQRITGLLNGVERYYGGGITIQFN"
     gene            complement(22699..23445)
                     /gene="zapD"
                     /locus_tag="U6309_000999"
     CDS             complement(22699..23445)
                     /gene="zapD"
                     /locus_tag="U6309_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEN5504452.1"
                     /translation="MHDHMITFQLATDFLSRIALRLEYLFKTINEACNESHEVIHRFA
                     LKNIIEIVEIVEKPELKSRFIKELIRIEHVLKKTNLLNHSELFDDLETQIHVLNHVPG
                     RFSNKIHDDEFLKTLRQIHHPNTKECEFNSPHLVLWFDSDPLMRQKTISQWVSCLKDL
                     EDTVKIYLSLLRGVTQYTPIRANNGFYQHSLSPKSLNHLILLRIDKKLKMIPKIQLGH
                     HSLTIRLYELVTGQEIRDKTIEMEIAFCQI"
     gene            complement(23684..>24038)
                     /gene="coaE"
                     /locus_tag="U6309_001000"
     CDS             complement(23684..>24038)
                     /gene="coaE"
                     /locus_tag="U6309_001000"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215598.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEN5504453.1"
                     /translation="ESLLHPVIRKKIEEQLIVCTSPYCLIEIPLLFNKHHYPYLQKVL
                     LVIAPLESQLDRIVKRDHCTKKQALAILATQPNLEQRLEAADDVLINEAGLSELKAKV
                     NKLHQKYLREAKIKQ"
BASE COUNT         7703 a         4037 c         4924 g         7374 t
ORIGIN      
        1 cagaataaag ttaaattccc aattcctttt ctaattggac agcagcattg cttctcgtct
       61 ctacgtaagg ctttatcaac gagtagaaat agtcctcgaa caaaaaagct taatcatatt
      121 tacgtgtaaa tatggcttga gaggatattc gaatacggca aggcccgagt ttttttcttt
      181 gctatgccca taatttataa aattttgaaa atattatttc aagactgatc attgggtgca
      241 gaaaaaattg aaggtgaatt gaaattgcga taacgcgtca aaaagagaat gttgtaaatg
      301 aaagaaaaaa tgatattaga caagtcaaat attataaatg ctatttttag taatctttcg
      361 gcggatgaaa tcaggaaagt caaaggcgta tcgcatgctt tttatgaccg ggccactgag
      421 tatttgacag gtcatctcga gaatgaaaat tttcacctta acagagttat tccaccttcc
      481 gatgcgatac aaagagagca aaaaattgaa ttttgtgcca aattttttca gacacttatt
      541 cgtagtatat ctaatggaga ggagggatca tcagggcaat tgtataaatt taataatatg
      601 atggaaaaaa tacgccgtgc tgtacaatct aacctgggtt taagtcgtgt tcaacgagaa
      661 acatatgaaa ctttccatcc tatttatgtc aaacttggag cgcccaattt tccaattaat
      721 gacgagggga aacttgatcc aggcagcatt gtttatcgaa ctgatcatgt taaagggaga
      781 gatgaaagaa aatcatcatg gctagtattg ttgaaggatg actcaaatga agacggactt
      841 tccatttgtt ttgatactaa atttcgtaaa tatctactac acatagatgg tgcaggtcat
      901 gttacctctc aattaacact tagatgggat gctatttatg acgatttatt gcctgagact
      961 tctatgcagc aaccacttag atcttttact ccctggcagc ggcatgctga ggcaaatttt
     1021 tttaatggac gttcaaccaa taaaatattg gggttattaa caaaaaatta cccggaaata
     1081 ttcaactatg atcctacctc ttattttaaa gaggaacctg tctttagtgt tgaattaaat
     1141 agaaatgctc caaaaaagct gtcaacagat aaaaagaata tttacttaac tttagatgaa
     1201 tcttttttta aaacgccaaa tggttttgag aatggtctaa acagacagcc aatattggct
     1261 gtgataaaaa caaactcccc actcagagga gattgttttt ctgtggtacc agccatttac
     1321 gaaaaacaga agcttcatat tctaattaag ggacatcaac agcaaactca agtagttgat
     1381 gtttttcatg caaattttaa aacagtagaa ctcctgtcat gggagttgct tgacgatgta
     1441 ataattttcg gagaaccctc aacagagtac aaacctgcat taactgattt aaatctttta
     1501 tccgatcaac aatccgagtt aatgtctcat gtttcaacgg caccaatggt aagaggaagc
     1561 ggacctggcg cccgacagta ttggttaaat gggtttttca aaagcacagt ttatagtgaa
     1621 atgattgtaa tagctgaaat tttcaataga cggtttatga gcacaccgtt agcgtccttg
     1681 gagcaaacac ttaatcattc agacgcaaca aaaagctttt atcgattgca atttagtcat
     1741 caggaattag aaaaggctaa agagtttgtt tttcagttgg ctatccccaa tgaattttcc
     1801 aagcaagaag attttgattt tttttatgga ggggacggca ataaattaga agaaataaca
     1861 aagcttgcta ttcttatgtt aaaaagaatg gtgcccaaag agtttcatca taaaattcgt
     1921 attggtatta atatgtgctg ccctgctgca tcagtggaaa acattggttc aggctttggt
     1981 ttgcgtaata agcctgattt tatagagcgc attgttaaag cgataaagga tgctgattca
     2041 aacttgaaag ttgaatttaa aactttaatg ttatcacatg aagacgtcta tggtgatggt
     2101 gatcaaatat ctggtaatac aaaaaatatc tcggaaattc agaatgaacg tctcacacga
     2161 atagtttata aaacgaaggc taatagattg gtgtatcaag gaaagccacg caatattgaa
     2221 gcttataacg tagatgctgt gtcatgtatt attagagagg tcaatacacc aaaggaattc
     2281 cagtttgggt atagcggaat ggtagctact attacaaatc atgatataaa ccaattaggt
     2341 tataaggttg tcggtgagcc acattacagt gtacaaagta ttcttgaaaa aattaaaaaa
     2401 caattagggg aagttccaat tgaatttgaa gtgatgctag gtcgagtttt gctaggctca
     2461 gcatgggttt tacaaggaag atatgccagt gatgcagaaa ttcttttaca tacgttgctt
     2521 caggcagcga ttgagcaaga atgggccagg gggatggggc gatatggtat tgatatatat
     2581 caaattcaag ttctatataa tattcgacat ataactaatg ataattatag gagttctcta
     2641 attaataaaa tacttaatgc tcaatctaca gaggaaataa ttgataagat atttgaacaa
     2701 tacttcagca ttaagaagaa aggggaatgt aatattttac agcttcaaag acttgaaaaa
     2761 gcggtatttg ctgcaatacc taatagcaaa gctcttcaac aatataaact aattacagag
     2821 ccaaactcaa aatcgaggac cggttttcaa tttttctcaa atcaagataa gaagcaaaca
     2881 ctagaggaaa aatctatctt cactccttaa gatagtattg taaattaaag tatggttgat
     2941 attgatagtt acattagata gttattcatg gtttaaaaag ttcttaacag gaaataatga
     3001 gaaagatgaa aaacaaaaat aagtctgtaa ttaccgttga tttaaatgat ccagtgaaga
     3061 gtctggaata tcttgaagag gctttccaga ttggtacttt tattctaaaa aattcgccgg
     3121 taaatttcaa aagattagcg aaggttttat ctgatctgga cacattttac aatttggatt
     3181 ttaaaaccaa aatcgaaatt tccggacaaa tttccggcgg tggtgcaacc agaggctatt
     3241 tcccactggg tatagaaagt ggtcttgttg atgtatttga aaaaaaagaa tcttactcgt
     3301 ttgggtggcc tgataatgaa attccagatt cagatacccc aaatttatca ttagttgccc
     3361 caaatatcta tcctgaacat ggttatatta atcaagatga tgtcggggga ttgataaaag
     3421 actttgccaa taccgcaaaa gaattgtcca agttaattga aatcatcctt ttcaatggaa
     3481 aagctgcttt accaatcgat gaatcttcag attttcaatc gttgttacga agcttcaggt
     3541 attataaaaa gactgagctt gaccctccta acacaggagc aaattcgctt cacacagatt
     3601 ggaacctgat tactttggta tatgcagatg ctgatgggta ccaatataga gataatgagg
     3661 gcaattttca aaacataatc ccagaaacag atgattgcct tatagttaat tttggagatt
     3721 ttttatcggc ttggactggt ggaaaaattg ttagtccttg gcatcaggta gttgaggcaa
     3781 agaaaaaaga tgtaagaaac tctctggtct tcttctatta tccagcggcc tctagctcag
     3841 ctgttaaaaa tcctatatat aatcaaggtg agcctcttag tttgttcgtt gatcagtctg
     3901 atcctttaaa taggttatgg gacttttcta aaatagaaac catgaatgaa atgtatgcag
     3961 aaaaatggaa acaagtgagt agaaaataga attcagttta agtcattaag caatgatcat
     4021 cataaaacgt attttttgct ggtacaattt ttttctaaaa aaatatggat atgctgttat
     4081 tagggttatt gttctctcct gatctcaaca tgtattagag tttagtgtac ttacatatga
     4141 gctcacttga ctcaagaata tgaccttgca tagcatattc aacatctttc tttgttgctt
     4201 tttcatccag agataaaaaa gtatccaaag catataacct aaaaaaatat cgatgtgttc
     4261 ctctaggggg gcatggtccg ccgtaacctg ttttgcccca actgttctta acgcttatcg
     4321 cggaatcagg caaatctata gcagcatcca gttcattata gcttgccggt atgttgaata
     4381 aaacccaatg aacccataat cctcctaacg catccggatc atccataatt atgacaaagg
     4441 atttggtatt tggcggagcg ttttcccaag caagcggtgg aggacaatcc aatccatggc
     4501 aagtaaattc atcaggaata aaatcgttat gctcaaatgc cgggcttgtt agtaataaac
     4561 tcaaaatact ctccttatct ctttcggata cagcattgtg aatcttaact ttttgttatt
     4621 tttccctact acccttaaat tatattagca tggtaatttt ggatctgcca ttcatcaaag
     4681 caataatgag attgaaaata ttgtgctctt cattgactct tactcctccc actcattagt
     4741 atatgaacct cttaaatatt tgccccaact ggagagtttt aatgaaggat ttattggtct
     4801 atgttttatg ttttattttt tcttcgttaa ttaatgtagc ttgggctgat aataatttat
     4861 gcattgctat caataatttt ggtacagtgc caatcaatgt ttccggcgca aattctatag
     4921 aggaacatac agtcaaacct agagaaacat taattttacc tgaaaaagag gtttctgctg
     4981 cttgttttcc tgattggggc tgtgatgtct ttattcttcc aatcgagcaa aatgctaaag
     5041 gattatatct taaacagctt cctaagggat cacgtattat ttatagtcaa ccagatttat
     5101 attatgtgaa tcgaactgct aatgttccat gcataccaga cctctgactc tttgcattgg
     5161 cacatgttaa taaaaggtat ctatcttgac gtcttacaaa gaaaaaaagg ttaaaatccc
     5221 aggttttact attgctctta aaatttggaa ttccgaaaat cctaatccag tattgtgctt
     5281 gcatggcaaa atggataacg cagcaagttt tgatttgctg gctcctttgt tatcagacag
     5341 gcaattggtt gcagtggatt atcctggaac aggattgtca agccactatc ctgaaggtgt
     5401 tgtcccgcat tggaaaaatg atgcttttct tatgtgccat gttatcaagg cattaggatg
     5461 gaagagcttt gatattattg ctcattcatt agggtctttc ctggcaaatg taataaccat
     5521 tgctcaacct aaactggtta agaaattggt atttttagat attttaggac caacggttca
     5581 tattattgag aacagtatgg cttatttacc cctaaatatt gaaagctatt taatatccgg
     5641 taaacagcca aggaccgtat ttagcagtct ggatgctgca atcaaggacc gtatgaatat
     5701 tggtaatata agctatcagg cagctcaagc cctggttcaa aggggtacaa aaaaagataa
     5761 tgagggctgg gtttggacat ttgatccccg gttacgctgc attagcgcaa ctatacctca
     5821 tgaagatgaa attcgagcca tgtttcgtgc tatagacgtg ccagtttgcc taattctcgc
     5881 taaccaaggt gtatcttatc ccgaatctgt ttttatagga cgttcccaat caataaaaaa
     5941 tttgaccatt catagggtac aaggaggaca tcatgtgcac atggatgatc cggcctctgt
     6001 cgcagaaatt atttctcaat atttaagctc caaataacac gggtttatta aagaataaca
     6061 agtatagatg gactcttgct ctaaatgttg atgcagggat gattgataat gaatgatttt
     6121 atgatgtata attggccaat ctatttaata gtggagcatt aatatgccta gatggtttag
     6181 aagaatagta gatgagggag aaaggctgct tgtcgaagta actgatgaat tgcgtgatga
     6241 aagtgaagtg cataaagcag caagtcagtt acatttacgt gttgaacaag ttttaaatgc
     6301 caatcgagct agacttgaaa aaccggttat tgaagcagct gaaggttttc ttcatgatct
     6361 aggagttatc ctggaagaag attccatgtt gagatttcct catgcaccag taaaccaata
     6421 tctactcgca gagaaatgca ctgatattat ttacaattac aaaccgtcct tagagtcagc
     6481 gccagggatt tggaatcaga tcaaagctca tattaataac tttattgaaa taattttcgg
     6541 tttagagaat gttttaccaa cgacgcagac tatgtttgca aaagatgtaa gtttcaccag
     6601 atgcaaaaga aacctttcca gattaaagga gtcacttgaa agtgacgcgc ctgatttgtt
     6661 gtctgtgttg gtcaatggtt aaatcatcat tgaatttaat taatttcctt aagcattaat
     6721 tttttcttga atgaccaggt actgtatcga catgcattgt cctggtcatc aaggtgtctg
     6781 gcttacaaga tacaactgag atggacctag gtgggtgatt caatcagttc ttttccatcc
     6841 tcttttttta agctggtaaa tatcaatcct gaaagaatcg taagtatccc taaaacaaaa
     6901 aaggttagat gaaaaatctt gactgttaaa acaaaatgct gcgaaacctg aaaagtgaat
     6961 aaactgacca gtagggcaga aattgctact ccaaaacttt gggataactg ttgaactgtg
     7021 ctcataatac ttgtcgcata actcatatca ttatcgtcaa tattcgcata cgcaagggaa
     7081 ttcatccctg tgtattgcag agcaatgaaa aatccataaa gaaatgttaa aaaaccaatg
     7141 tagatgacag aagtgctttg atttattgtg gcaaaggacc agagagaaaa acagactaaa
     7201 acggtattta aaaggagtaa ttttttataa ccgaatcttc gtaagatggc gaaagataat
     7261 ggcttaacca ggaatacgcc taatgccact ggtgtaagca acattcctga taactgggga
     7321 gaatattcta aaataatttg gaagagcaaa ggtaataaaa agggaattcc accaaaactt
     7381 aaacgagtta ataaatttcc aaacacggca atacgaaatg ttcttatatt taacaaagta
     7441 acttttataa taggattcct tttgctacga gagtgtttgg tatatccaat taacagcaag
     7501 gcggataaaa gagtaattga agcggagtag acgatatccg ctttcgtctc actaaacaaa
     7561 gacaaaccta aagtcaagaa tgcaaggccc gctccgaaca ttataaatcc tgatttatcc
     7621 aaaggcggaa ccgatctggg caggatagtt ggaagcagtt tgtgagataa aagcatggcg
     7681 attatgccaa cagggatatt aacccaaaat atccaccgcc atgagaaata atcagtaata
     7741 ataccgccca gtaagggacc aagtagcata cctagcgagg caatcatgac gacaatactc
     7801 atttttgtta ttaattcgtg acgctcacaa gttctcagaa taattaaacg tccaacaggc
     7861 acggttaggg agccgcccac accctgtata attctggcta atactaattc ccacaggctt
     7921 tgagtgaatc cacaccataa ggaacttaga gtaaagacac caatagctgc tataaatact
     7981 ttttttacac catatttatc agcaatccat ccacttatag gaataaagat tgccaagctt
     8041 aaaagatagc taatgagcgc gagtttgaga tcaattggat tgacctctag actatgagcc
     8101 atcaccggga ttgcggtgtt gagaatagtt gtatcaatag cctccataaa catggcgcat
     8161 gatacaatga acataataat atttttcttc ataatgcctg ttttatggaa gttagaacgt
     8221 ttaaatttta ttaacctgaa actctattaa agtcacaagc ccgctccagt attttgtcca
     8281 atggtgatca tagtgatcct tattattaac tcgtttaagc aaaacgccgt tttcaacttt
     8341 aaataatgga gctgtatcat ttatctgctc aatttttgag tagtcgggaa acacatccat
     8401 ctctgggtag tgttcaggga taactccatt cataaaagta ctatggatac tgtctgcaga
     8461 acgttgcatg gcatctaaga tgatcactct ttgtaattcg gcttcaggtt tgtataaata
     8521 gccatctcca taggcaagcc aacttattcc ttcatgattc actacattta acccatgttg
     8581 attatcttca ttatgcatca aattggcgat aagaagattt aaaacagaag gcagtagtat
     8641 atcatgtcct atggctcttc ttggagtacg catgtgccct gcagaaaaag aatcagttaa
     8701 gtaatgattt gcaaaggcgt tttgagcata ggctaattca aggagcttgt tagcttcatc
     8761 gataagcccc tgctgctttt tctcataagc ttttaatgct gtttccaaag caaatctatg
     8821 gcctgtttta taggctacaa gtgaatcggg aacaaagtga tcatagttga cttcagctaa
     8881 tttaatataa ttgccaaagg gtataaaacc actgattata cttccaccac aagtcagttt
     8941 gtttaattcc ttgctaatag ccgagtcgtg ctgcttataa taatcccaat cgtttgttcc
     9001 gttttgtttt gatttttcga tctcttcagc caaattggta aaaaaccgat ccagatgagt
     9061 tatctgatta agagggttag agcaacgttt gtcttgcttg ttacctaaag tccccagagc
     9121 gtcaaactgc gccttgaaac aagctaacct gtttttctca gagcaattgg atatgggttt
     9181 tgctggattt ccaaacatat caccaccgta catcacgatc tcgccataac ttagcttact
     9241 cccattgggt aatctcagtt ttacaatacc agtcttgctg ttatggcctg gcaaataaag
     9301 ttgaacccta tcccccagat ttttatgttc cagattggca aagcctgcac caaatttttc
     9361 taaaaattga atggtcgttg gttgagatat tgtctcgata gaagatgctt cattggcgca
     9421 gatgttatgg cttattaaca atgacaaaga caaagctcgt gtgatattct tcaacattat
     9481 gcattccctt gattatttgg ccctttattt gggcattttt tgttttttat atccatacaa
     9541 cattctaaaa atgtagcatg agaattttgt aattgataat tatttgagaa tcatttcatg
     9601 agaaaagtta agccaaaatt gaatttaacc tcacaaaccg ctcggatcgt aaatttaagc
     9661 catgatggaa gggggatagc acgagttaat ggcaaagcaa cttttattca gggagcatta
     9721 cccggtgagg tggttgaatt tcagtataca cgggtaaaaa aggattttga tgaaggtaaa
     9781 ttattatcaa ttgtcgagcc ctctactctc agggttgaac caaaatgccc tcattatcaa
     9841 atgtgtggag gctgttcttt gcaacatatg agtgccgagg aacaaattcg tttcaaacaa
     9901 tcccatcttt tggatttact atcccggtat ggccatactg aacctcaatc agtgttgtcg
     9961 cctttaacaa gccatcattg gaattataga aataaagcac gcttaagtac tcgctttgta
    10021 gaaaaaaaac aaagcaccat ggttggattt agagagagga ataatccaag gttcattaca
    10081 gaaataaatc aatgtcctat tcttaattca aaaatagata ctgatattgt ccatttgaga
    10141 aaattaatcg atacgatgga agataagcag tgtatcgcac aaattgaagt ggctgcaggt
    10201 gataatgaag tggcgctgat tttccgcaat ctatctccat taacagagca agatgagttg
    10261 aaaatcaggg aatttgcgca acaattccaa tacaaggtat tcctgcagcc aggtggactt
    10321 gattcggttt tttgttttta tccttcagat gctcatgctt atttgagtta tgagctgccc
    10381 gattatcaaa taacttttca atttcatccc aacgatttta ctcaggtaaa cgctgagtta
    10441 aacagaaaga tggtaacgca ggcaattcag ttaatggaat taaaaaattc tgatatcgtg
    10501 ctagacttat tttgtggact gggtaatttt tcacttccca tggctaaaca ttgctctcga
    10561 gttatcggtg tcgaaggcaa taagaacatg gttgaaagag cctatatgaa tgccaagtct
    10621 aatcatatta ccaatgtgga tttttatgct gctaatcttg atgatgtgat ggaagttagg
    10681 aatctggtta atacgtcttt tagcaaggtt ttaatcgatc ctccccggtc tggtgcgctt
    10741 gaaattgtaa aacaaattga ttcaattgat cccgagcgta tagtatatgt atcttgtaac
    10801 ccgataacat tagctcgaga tacagatata ctggttaatc aaaaaggtta cgttttgata
    10861 acagctgggg taatggatat gtttcctcat acagcgcatg tcgagtctat tgccttgttt
    10921 caaaaaggat aatatgtatg gtaagagtaa aagatacgac tccgttgacg ccagatggca
    10981 gtattgacgt agagatgtgg ttacatcatc ttggctcaaa aggatatttg gataatctcg
    11041 aactcgtaag agctgcctgt actttaagtc aattggctgg tcaggatcat gctactgaaa
    11101 cagggcaaac ttgtttacaa caagggttat caatggctga tttacttgct gacctggaag
    11161 tagatcagga aactctggct gccgctatta tttttgaaaa tgttcattat gcggatttat
    11221 ctattgacga tgttgaagag caattgggac acaatatcgc taaattggtc aaaggcatag
    11281 aaaaaatgag tgccatgaac aatttccagg ctttaaataa atacccgcaa aacaaaaatc
    11341 aaattgacaa cattcgtaaa atgctccttg cgatggttga tgatgtgcga gttgttttaa
    11401 tcaagctggc agagtgttta tgtattttaa gaacagcagg gcatctgtct gaaacggtac
    11461 gtaaacagct ggcaactgaa gccatggaaa tttacgcgcc tttagctaac cgcttgggaa
    11521 taggggcaat caagtgggaa atggaagatt tggctttccg tcatttgcat ccagaagaat
    11581 ataaggcgat tgccaaggga ttgaaggcta aacgattgga aagggacagc tttgtcaatc
    11641 gcattgtcga acagcttgaa catcagattc gagcaacagg agctcgccac tttgccgttt
    11701 atggtcgctc aaaacatatc catagtattt ataaaaagat gaagcgcaaa aatgtatcat
    11761 tagacgagat atatgatgcc acagcggttc gtattctggt agatactgaa gcacaatgtt
    11821 acgaagtgtt aggcatggtt catactttgt ggaagcaaat tccagcagag tttgatgatt
    11881 acatcatcaa tccaaaatcc aatggctatc agtctcttca taccgcagta gagggtccgg
    11941 aaggaagagt ttttgaagtg caaatcagaa cttttcatat gcatgattta gcagaaatgg
    12001 gtgtggcagc ccattggaaa tataaagagg gtgcggtatc acaaaaagag agccatgagc
    12061 gcaaaataga atggttaaga gacgttttgg cgtggcacca ggaaatggcg gctaataaag
    12121 gtgtcgcaga aaacgttacg actgagtttc ttgaagacag agtgtatgta tttactcccg
    12181 atggtgatgt gttggattta ccccatgggg tgactccttt ggattttgct tatcatgtgc
    12241 atagtaattt gggacatcgt tgccgaggtg ccaaggttaa tggtcatatt gtcccattga
    12301 cttatcaatt gaaaacagga gacagggttg aagtactgac tggaaaagaa atcaaaccat
    12361 caagggattg gattaatcct catctgaatt atttaaagac cgcaagagcc aaggctaaag
    12421 tattgcattg gtttaaaatg caagattatg aagataatgt gagagatggt cgtgagttat
    12481 tggaaaaaga attaaaatca ttaggtatta aatcggataa actcaatgaa gttattacgg
    12541 cattgaatta taaaaaggcg gatgatcttt atgccagctt gggacgaggt gatattaaaa
    12601 taggtcagat tgttaatcga ttggcacctc cagatacttc agaacaaaat ttacagaagt
    12661 ttgtaagaca acaacaaaaa ccagaggtca cagggagtga tcttcgtatt gagggagttg
    12721 ggaacttatt gacatttatg gcgagatgct gccaacctgt tcccggtgat gaaatagtag
    12781 gatatatcac gattggaagg ggggtttccg ttcatcgaaa agattgtcct aatattatac
    12841 atgccagtga aaagcaaaag cgacggttta ttcaggtgaa ttggggtaca gcaacacggg
    12901 aaaattatgt tgttgatgtg ttgattaaag cctttgatcg ctccgagtta ttgaaggatg
    12961 ttacttcgct gctttcaaat gaaaaagccc atgtttatgc tttacagacc cagagcaata
    13021 aacatgaaaa tatggcttat atcactttaa ctgttgagat cgatggttta aacagtttgt
    13081 cgcgcttatt aactaagttg gagcaaattc ctaacgtgct ggaagcaaga aggcaattat
    13141 aactttgtat atttagccgg agccttctca aaaaattggt attagaagat aaaccctgca
    13201 acaaccgctc tgtgttcgct caataggacg ttataagttc tacaagcggc acctatggac
    13261 atgcattcga taccaattct tttttgcgat aattgactga ttagagtgac aggcagcgtt
    13321 ttgcctgttt gcagatgacc aataataatt aattcgggtt tgcttcttaa taataaatca
    13381 atatatgaat tattaatgtc ctggatgtct ttaattacaa aatcagtaat gatttctttc
    13441 ttagaaacaa ttaaactact ttcataaatt atggaattaa tctgaatctt tttatcgctg
    13501 tatgcttgca ctgcatgttg ctctgcggtt tctagattga tattcatgat tttaaaaagt
    13561 tcattagttt ataatggcta acagtatatc acagaggtca atattatgag tcaatttcca
    13621 cgcattaaac gcttaccccc ttatgtattt aatacaataa accagcttaa gatggaagcg
    13681 cgggcgcgag gagaggacat tatagacttt ggcatgggga atcctgatca accaacccca
    13741 cctcatattg ttaataagct tatagaggtt gctcaaaggc ctgatacgca tcgatattca
    13801 atgtcgaaag gaattccgag attacgtcga gcgatggcaa cctggtatct tcgacattat
    13861 gatgttcatc tggacagtga aacacaaata ttagctacaa taggatctaa agagggactt
    13921 gcacatttgg ctttggcaat ttcaggtcca ggtgatacgg tgcttgttcc tgaccctgct
    13981 tatcctatcc acacctacgg atttattatt gctggtgcta atgtaaagca aattccttta
    14041 atcgatgaag ttcaattttt ggctgcagta gaagatgcta ttaacagaac ctggccaaaa
    14101 cccaaagcct tggttttgaa ttttccggca aatccaagta ctcactgtgt cgaatatgag
    14161 ttttttgaaa aagtggttga tcttgccaag cgtaataaca tttggattat tcatgatttg
    14221 gcctacgcag atatagtttt tgatgggtat aaagctcctt cgattttaca agttccaggt
    14281 gccatagaca ttgcaattga aacttactca atgtccaaat cttataatat gcctggctgg
    14341 cgtgttgggt ttgcttgtgg taatgaagag ttagtagctg ccttaacacg tataaaatcc
    14401 tacttggatt acggtacttt tactcccatc caggttgctg caattacagc actggaaggc
    14461 cctgatgatt gtgtgttgga aataagaaat ctttatgaaa agcgacgcaa tcttttgtgt
    14521 gatggactga atgacattgg ttgggaagta agcaaaccta aagggaccat gtttgtttgg
    14581 gctcctattc ctctcaatta ccgcgctatg gggtctcttg agttcagtaa atatttattg
    14641 aaagaagcac aagttgctgt ttcacctggt ataggttttg gacagcaagg ggatggttat
    14701 gtccgattcg gcttaattga aaataaagac agaataagac aggccttgag aaatttaaaa
    14761 gctctgttta aaagagatgg tttactaaag gctatttagt gcaatgaatg tcattataaa
    14821 cacagagagc gactgtgata gcaaagaggc caaattatta aaatcgcaag gtttggcagt
    14881 aattaattta ttagcatgcg gcggttatga ccttgtaaat cccccagtag gtaacttatt
    14941 gaaatcgagc cataatcttg agggagattg ggtggtttta actcctatgc attggcaagc
    15001 atcacataat gatgctgtca ttgtcgctct tgataaggat ttacgagtaa cagatgaaga
    15061 ggtgaaatat tggttcgatt tgtattccgc ctttttagcg gaagaaggaa tgtcccttta
    15121 tttttatgac aaatacactt ggttattgcg agttgatgat aagccacctc ttaatgccaa
    15181 accaatttat caggtactaa acaagtcttt gatgccagaa ttatcgcaac tagatgagac
    15241 tatgtactgg caaaaatttt ttactgaaag tcaaatgttt ttctcttcca atgctcagaa
    15301 atcattaatt aatggtgttt gggcatgggg tagtgggaaa ttgaaaacta aaaaaacaat
    15361 atcaatttgc acagacaagc attttctggc agtagctcaa gcatattctt caaatgtgac
    15421 tttatatgac ccgttagtga acgtgagcgg gtttgaggtt gttctattac atgacattaa
    15481 ttctttaagc gaattacatc aagttgaaat aaaaaaaata ccggcacatt ggtactggaa
    15541 caattgtgtt ttaattaagg caaaatccca ttggtttact cgattatgga gaagtctgac
    15601 acatgctgat taaacaacga caacagcctg agtttactgt taatttacct aatgtacctg
    15661 atgtattaaa acgtatttat ataacacgag gcattagtga cgaatctcaa cttgataaac
    15721 aattacaaac attacttcct tttcatactt taaaaggcat taatgaagct tgtctaagat
    15781 tagagaatgc tttgcgttca gagcaacgta ttcttattat cggtgatttt gatgccgatg
    15841 gggcaacttc tacggcatta gccattactg ctttgcgtgc tatgggtgca aggtttgttg
    15901 agtacttggt acctaatcga tttgaatttg gttacggtct tactcctgct attgttgaag
    15961 tggcaagcaa atggcaacct catttaatta ttactgttga taatggtata gccagttttg
    16021 aaggagtaga tgcggctaat aaaatgggaa tagatgtatt aattactgat catcatttac
    16081 cagcagaatc actacctgat gcttgtgcaa ttgttaatcc caatcagcct ggttgtttat
    16141 tcccaagtaa gtcaattgcg ggagttggcg ttatttttta cgttatgctg gcattgcgca
    16201 ggcatctttc caattgtaac tggtttaatg aaatcaacat gcctgagcct aatatggcga
    16261 gttttctgga tttggttgca ttagggactg tagccgatgt tgtggggctt gaccaaaata
    16321 acaggatcat ggttaatcaa ggcatgatgc gtattcgtca aggacaatgc agagagggaa
    16381 taaaagcctt gattgaaatt gctggtaggg attgctccag gctaagagaa tctgaccttg
    16441 gttttgccat agcgccaaga ttaaacgccg caggccgtct ggatgatatg tcgcttggta
    16501 ttgaatgttt gattacgacg gatccaaaaa tggcaagaaa tttctgtcaa cagttggatg
    16561 agttgaatct ggaaagaagg caaatagaat ctgaaatgaa agagcaagcc atgcttgctt
    16621 tggagaaatt gtcaattaac gatgaagaga ataaacactt accagttgcc ttgtgccttc
    16681 atgataaaac ctggcatcaa ggagtgatag gaatcttggc aggaaggatg aaggaaaaat
    16741 atcatcgtcc tgtcattgca tttgcttctg tcagtgagca tgagttaaag ggctcagcac
    16801 gttctgtccc cgatttaaac attagagatg tattggcagc tgttgataaa gacaatccag
    16861 gactgattct taaattcgga ggacatgcta tggctgctgg cttaagtatt agccccgaat
    16921 tattgaatga ttttcgctca gcattcgtgt cagaggttct gaaacatctt gacttatcac
    16981 agtgcgaagg agaaatactg acggacggac cattgcaatc ccatgaactg agtttggaaa
    17041 cagcacaatt aatacaacaa gctggtcctt ggggccaagc gtttgcagaa cccgtatttg
    17101 ataatgtatt tgaaattctg gatcaacgaa ttgtcggtaa aaatcattta aaaatgacat
    17161 taatttcctc acaaggaggg gagccactgg atgccattgc ttttaatgtg gatttaaaat
    17221 cttggcctaa tcataggatt aagtacattc atgccgccta taaactggat atcaattttt
    17281 atcaggggag aacaaggctc caattgctca tccaggcaat gaatcctgtt cagcaagcat
    17341 aaactcttaa attgatgcta tttttgtgta tttctcaaac aacaatgtat aatgttatat
    17401 ttgctttaaa ttgatattaa acgtggttaa tagtattttt tccaaattat ctatagcgcc
    17461 gatgattgat tggactaata gtccatttcg tactctgatg aggttattgg ctcctaacgc
    17521 ccttttgtat actgaaatgc aaacaacagg ggctgtgcaa aataatcctg acagagcttt
    17581 gtattttaat tctgttgaac atcctatcgc tattcaattg ggtggggcag ataaatccgc
    17641 attggttaag tgcgccatta ttgctgaaca gaaaggtttt aatgaaatta atttgaattt
    17701 gggatgtcct agtgacaaag tacaggcggg acgatttgga gcctgcttga tgaaggagcc
    17761 agaacacgtt gctgattgta ttcgtgcaat aaaacaggta gtaggaatac cagtcacagc
    17821 gaaaacgagg atcggtattg ataaccagga cagttatgag tttttcagat ctttttctct
    17881 tcacttggtt gaagcaggct gtgataagct tattattcat gctcgcaagg cttggttaaa
    17941 tgggttaaat ccaaagcaaa atagaacgat tcctcccgta aattatgatt atgtatatga
    18001 gctaaaaaag gaaattcctc atatctttat tacaatcaat gggaatatat taactattga
    18061 agaaataaac agtcatttaa agcatgtcga tggagtaatg ttaggacggc ttgcttgcga
    18121 taacccttac caaattgctg aaattcatca agccttatac ccagaattca ccaaaattaa
    18181 acgtagccag ttgttaatga attatctgga ttattggttt gatcaatctc gagccaatct
    18241 ctcgttaagc attttggtta agccactttt taatttagct tttggattac cgggagccaa
    18301 acattggaaa aagaaattga tgaatattat tcaggagaaa aatacttccg gttttagtga
    18361 gttaatccag tatttattaa atttggaagc aataaatcaa gtcgagtttg tataagaaat
    18421 aatagagcag atgtcaacat ttcgtggaat tttatcaaga tttctagtac attaaccagt
    18481 ttattaattg gaacatcctg agggttattg aggccatgct gagtacgtta attaagaaaa
    18541 tgtttggaag ccgaaatgaa cggacattac ggcgaatgga aaagtctgta atggctatta
    18601 atgcgtttga gccgaaaatg caggctttat caaacgaaga gctggctgga aaaactcaag
    18661 aatttaaaga acgtttcaat aatggtgaaa gtctcgatga attacttgcg gaagcttttg
    18721 ctacggttag agaagtatca ttgcgcactc tcggtcttag gcattttgac gtacaattaa
    18781 taggcgggat ggtattgcat gagggaaata ttgctgaaat gcgtaccggg gaaggtaaaa
    18841 cgttggttgc aaccttgcca gcctatctca atgccattag cggacgtggt gtacatatag
    18901 tcactgttaa tgattatctt gcgaaaagag atagccaatg gatgaagccc atatatgaat
    18961 ttttagggct tacagttggt gttatctatc ctgatatgtc gcacaaggaa aaacaggaag
    19021 cttacaaagc ggatattgtt tacggaacta ataacgaata cggttttgac tatttacgtg
    19081 acaatatggc ttttagtctg actgataagg tacaaagaga gcttaatttt gctattgtcg
    19141 acgaagtgga ttccattttg atcgatgaag cgagaactcc attgatcata tctggcgcgg
    19201 cagaagatag ttcagagctg tatatcaaaa ttaattcttt gatcccacag ttaaaaaaac
    19261 aagaagaaga gggtgacgaa ggcgattata cggtagatga aaagcaaaaa caggctcatc
    19321 tgactgatgc aggccatcta catatagaag aactgctgac caaagccaaa ttattagatc
    19381 ctggtgagag tctttatcat gccagcaata tcatgctgat gcatcatgtg aatgcggcac
    19441 tcaaggctca tgcaatgttt cacagagata ttgattatat tgtcaaagac aatcaagtgg
    19501 tgatagtgga tgagcatacc ggccgtacca tgcctggaag gcgttggtca gaaggattgc
    19561 accaggcagt cgaagccaag gaaggagtcc caattcaaaa tgaaaatcag actctggctt
    19621 ctataacttt tcagaatttt ttccgcatgt acaataaatt gtcaggaatg acgggtactg
    19681 ctgacacaga agcgtatgag tttcaacaaa tatataatct tgaagtggtt gtaataccta
    19741 ccaataggtc aatgattaga aaagacgagg ctgatttggt ttatctaaca caggcagata
    19801 aattccaggc gataatcgaa gatgtacgag aatgtggtgt tcgtagacaa cctgtcttag
    19861 ttggaacagt ttcaatcgaa gcttctgagt ttttaagtca actcttaaaa aaggagaaca
    19921 taaagcatca ggtattgaat gcaaaattcc atgaaaaaga agcccaaatt attgctgaag
    19981 ccgggcgtcc tggcgctgta accattgcga ctaacatggc tggacgagga acagatattg
    20041 ttttaggtgg tagcttggca gctgatctgg ctaatttgcc agctgatgcg agtgagcaag
    20101 aaaaagaagc agttaaaaaa gaatggcaaa aacgtcatga tgaagtgatt gcagcaggcg
    20161 gacttcgaat tattggctct gagcgtcacg aatcaagacg tatcgataat caattaagag
    20221 ggcgatccgg tcgccagggt gatcccggaa gtagccgttt ttatttgtca cttgaagaca
    20281 atttaatgag aatatttgct tctgagcgcg tggcctctat gatgcgtcgt ttaggaatgc
    20341 agccaggtga gccgattgaa catagtttgg ttaccagagc aattgaaaat gctcaacgca
    20401 aactggaagg gcatcatttt gacgtaagaa agcaattgtt ggattatgat aatgtagcga
    20461 atgatcagcg gcaggtcatt tatactcagc gttcctctat tatggcaatg acggatacac
    20521 aggaagttgt tgaaatgatg agagaagagg taatcgatag tctggttgat acttatatcc
    20581 cccctcaaag tctggaggat cagtgggatc cacaggcttt gtctgatgtt ttgtcagatg
    20641 aatttaaaat caaagcctct gtgcctgact ggattgataa agatcatagt atacaacctg
    20701 agcaaattaa agaaaaaatt cttgctttag ctatagagca ttatgatgaa aaagtaagaa
    20761 aagttggtag gccagtcatt tcacaatttg aaaaatcaat tattttacaa actttagata
    20821 atcattggcg tgaacacttg gccgcaatgg atcaattacg tcagggaatt catttaaggg
    20881 gttatgctca gaaggatcca aagcaggaat acaaaaaaga agcttttagt ttgtttacaa
    20941 tgatgctgga taatcttaag tacgaggtga tcaggatact atcatccgtt gaaattcaaa
    21001 cggaggagga tgctcaagtt gttgaagagc aacgtagagc ggatcagatt agaaaaatga
    21061 atctcatgca tgaaagttta tcagagaatg atgaggcaag tgaaacgcaa acttttagac
    21121 gacaagaaaa gaaaattggc cgtaatgatc cttgcccctg tggctcaggt aaaaaatata
    21181 aagcctgtca tggaagcctc gtgtgaaagt agcagttgct atcattatcg atgaaaaaca
    21241 acgtatacta attacccaac gtccttttca tgtcgcacac ggaggatttt gggagtttcc
    21301 tggaggaaaa ctggagcctc atgaatctcc tgaggatgct ttggttcgag agattaggga
    21361 ggagttaggc ataattgtaa atgaatatcg ttttctgggg tatgtcgatt atgactatcc
    21421 agataaacac atacaattaa ttatttttat ggtgactcgt tttacaggca accctttatg
    21481 tcaggaagga cagctcaata tgaagtgggt aaaaaaagag gaattaaata ttaatgattt
    21541 ccctaaagca aatcatgcgg ttttcgattt aattgatatc cctgaacttc aatatcaagc
    21601 tttatgaatg tgcgaatagt ctaaagttta aagctaggac atcgtattta attgctaagc
    21661 acaaaataaa ctccttgttt agatgatgtt ataacagatc agttgtcgag ataaaatcaa
    21721 tcagaatggt cactacaagg tatgttcaca tagactagag attgcaaaga tagtagcctt
    21781 ttgtaaatga ttagattttg tatttcaatt aattgttaat caattggaca gaaaaatgat
    21841 tactaagcaa aatggaattt atacacttac cctttcttta ttactattct cacttcaaat
    21901 caaagctgct acagattatc aaacatatga aacagtttgt ggtgaaccaa cagatatgct
    21961 ttcttttctt gaaagaggtg gtattgcaac taatccttgt gtagttcctc ctaagagcgt
    22021 attaattagc tctggatatc aatatcagca attaattggg gaaggtgtac agcataattt
    22081 tcctgctgct gcaatacagc taggtttgcc tggttatttt gaggtagact tgttgttacc
    22141 caattatatt aatcagacgg ttgatccacg cataggtttt agtgaaactc agcttatcgt
    22201 caatcatgtt ctatggttta acaataagtg ggttattact gctagcggaa catttgtatt
    22261 tccatcaggc agtgcttctt ttggtagtcc aaatccagga ggtggtgtaa taggaatttt
    22321 aagttataac tttaattccc aattaaatct tacaggtaat ttggggatta cttctcaatc
    22381 ggagcctagt tacgatggtg gacaaagtta tacgagtgtt aatccagatc ttattttatc
    22441 gtggactaaa aataaaatat cattatttgc agagatttat gggcaaagta aaacagcacc
    22501 tgatgaagga agcggtttta gtactgatgc aggagttctt tatcaagtta aaaagaatat
    22561 tgcaattgat tttgaggttg atcaacgaat cactggcctt ttgaatggtg ttgagcgtta
    22621 ttatggcgga ggaattacta tacaatttaa ttaatgcgga ctcactttaa cgcaaagcca
    22681 gcatagcgca tagggcttct atatttggca aaatgcaatt tccatttcta ttgttttatc
    22741 tctgatttct tgaccagtga caagctcata aagcctgata gttaaactat gatgccctaa
    22801 ctggatttta ggtatcattt tcaatttctt atctattctt aacaagatca gatggttaag
    22861 tgatttaggt gaaagactat gctgatagaa tccattattt gctctaatgg gagtatactg
    22921 agtaacacct cgtaacaatg acaaataaat ttttacagta tcttccagat ctttcaggca
    22981 tgatacccat tgactaattg ttttttgcct cattagggga tctgaatcaa accacaatac
    23041 caaatgaggg gaattaaatt cgcattcttt agtattgggg tggtgtattt gtcttaaagt
    23101 ttttaaaaat tcatcatcat ggattttgtt actaaatcga cctggtacat gatttaacac
    23161 atgaatttgt gtttccagat catcaaataa ttcactatga ttcaatagat tagttttttt
    23221 aagtacatgc tcaattctaa ttaactcttt gataaatctg ctttttaatt ccggcttttc
    23281 aactatttca acaatctcaa ttatattctt taatgcaaat cgatgaataa cctcatgaga
    23341 ctcattacaa gcttcattga tggttttaaa aaggtactcc aggcgcaagg caattcttga
    23401 taaaaaatca gttgctagtt gaaacgttat catatggtca tgcatagatt aaggccatcc
    23461 ttagctttat cctataataa tggcaattta aatttttata gatgcacatc gggtaaaaat
    23521 gggctcctgg gtttatttta aaaacaatat tgttttttat aaacaaatct aaaaaatcaa
    23581 gctattaagg tgaattttgt gttaagacta acataaaagt tgttgaaaat aaaaaaatct
    23641 ttaaaatcca tatattaact ggtcttttgc tagaaattta ttgttactgt ttgatttttg
    23701 cttccctaag atatttttga tgaagcttat tcaccttagc ttttaattca cttaatccag
    23761 cttcattgat cagcacatca tccgctgctt ccaagcgttg ctctaaatta ggttgtgtgg
    23821 ctaaaatggc taatgcttgc ttttttgtgc aatgatctct tttaacaatt ctgtctagct
    23881 gagattccag gggagcaata accaatagaa ctttttgaag ataggggtag tgatgtttat
    23941 taaacaacaa aggaatttca ataaggcaat agggcgaagt gcagacgatg agttgctctt
    24001 caattttttt acggatgact ggatgtaaaa gactttca
//
LOCUS       DASLOE010000031        23855 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.031, whole genome shotgun sequence.
ACCESSION   DASLOE010000031 DASLOE010000000
VERSION     DASLOE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 23855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23855
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.031"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(169..1026)
                     /gene="queF"
                     /locus_tag="U6309_001001"
     CDS             complement(169..1026)
                     /gene="queF"
                     /locus_tag="U6309_001001"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEN5504454.1"
                     /translation="MNKELESIYQNIANQSELGQTANYDSYYNPKRLYPIPRAPKRQE
                     INLDPNSTTFYGFDCWNHYEVSWLNSKGKPVVAMAVISYDCHSPCIIESKSLKLYFNS
                     LNNSTFPDVETVVQTISKDLSYCIGSEVAVNVYPLSDIPNQTIYAAFDGVCLDKLDIE
                     CSVYHVMPDFLSTSSELVEEVLYSDLLKSNCLVTNQPDWGSVQIIYKGKKINHEGLLK
                     YLISFRNHNEFHEQCIERIFADIMRFCQPESLTVYGRYTRRGGLDINPIRSTEPCAFD
                     GQNIRLIRQ"
     gene            1171..1572
                     /locus_tag="U6309_001002"
     CDS             1171..1572
                     /locus_tag="U6309_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEN5504455.1"
                     /translation="MINQQIEVFLKSPAFAVVGASSNREKYGNKVLRCYLRNGKKVYP
                     VNPHETLIESLPVIHSVNDLPDDVHSISIITPPSITEEVVKEAVKKGIQHIWMQPGAE
                     SDSAIQYGLDHKLNVISGGPCILVVMGYEEN"
     gene            complement(1637..2758)
                     /locus_tag="U6309_001003"
     CDS             complement(1637..2758)
                     /locus_tag="U6309_001003"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEN5504456.1"
                     /translation="MPLQPFKTSNLLTMGVELELQLISLSNFDLTAASPDILELLGRS
                     SFPGSFTPEITESMLEIATDVHEEYDQLLKQLFHIRDALVTVGDRLNIGICGGGTHPF
                     QMWSDQRIFNKTRFIEVSELYGYLTKQFTIFGQHIHIGCEDGNQALFLLHSLNRYIPH
                     FIALSASSPFVQSKDTLYNSARLNSVFAFPLSGRAPFVLNWDEFSLGYFEKMEHTGIV
                     KSMKDFYWDLRPKPEFGTIEMRVCDSPLTVERAAALACYMQALCSYLLENKEPLPHED
                     DYLVYNYNRFQACRFGLDGTLVHPKTYEQILLREDILTTLRRLKPYANQLNSTMALEH
                     IYEITHKGSDASFLREKYAEHRTLESVVNESLKQFRRSK"
     gene            complement(2760..3947)
                     /locus_tag="U6309_001004"
     CDS             complement(2760..3947)
                     /locus_tag="U6309_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN5504457.1"
                     /translation="MIDVGKIITDLAWPISLLIAWFAGELGYRWAKLPHISIYAIVGF
                     SLASTQIGLLPSIQPPSILLLANIGFGLILFESGYRINLRWLSYNPWVAASSITEATL
                     TFCLVYILVKSFGLQTSTCLLLASLSMATSPATVVRVINEQRSSGQVTERVLHLSVIN
                     CVLAVFLFKVIIGLVVFRTSGNLWEAAYASLTVTAISTIWGIIFGVIMPILLKTVKST
                     CQDNTLAFACAVILLVILTHNLKLSPVLATLTFGLVSRHGRMVFSPSQPGFGTLGDLL
                     SVLLFVFIAATIEWQRVVTGVWIGLSIITVRFVAKIIGISLFAHISGISLKKGILTGL
                     AMTPISVFVILILEQSRYIGINLVDTLAPLAMVALTLEILGPIMVQRALIKANEISAI
                     KEF"
     gene            4277..4879
                     /gene="def"
                     /locus_tag="U6309_001005"
     CDS             4277..4879
                     /gene="def"
                     /locus_tag="U6309_001005"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN5504458.1"
                     /translation="MALEIITIEKKENYHMLKTRAENIQFPLNREDKDLIEAMKKKLY
                     ALGGVGLAAPQVNSAKQIIAVYIPEEAQLLRDNAKIFPMHILINPNYEPLPHTRIISD
                     FEGCYSVSSKAGKVPRYDEIQLKYYDEEGNVHQQIENGFYARVLQHEIDHLNGVLITD
                     RLTPDCVQGTIEEMMTIRRAELPVEKRKLFDQIMAKKLKK"
     gene            complement(4915..5319)
                     /locus_tag="U6309_001006"
     CDS             complement(4915..5319)
                     /locus_tag="U6309_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HEN5504459.1"
                     /translation="MHKLIELQGVGPKYAEMLKRVGIESQDDLLRVCSGRRGRFRVAE
                     QTGISHKLILKWTNQADLARVNGINEEYAELLEQAGIDSIPELAHCNPEQLYSSLKEK
                     NEQCHLAQHVPGLSQVVTWVEQAKKLPRAVYH"
     gene            5507..5851
                     /locus_tag="U6309_001007"
     CDS             5507..5851
                     /locus_tag="U6309_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504460.1"
                     /translation="MRKHKNKMVRIFLLTLGLFSSTTINAWYGSGITIGVARANVHYY
                     GDDIRVYDPWGYGYYDRNYYYNGVRNIIVPNVIVPNVVINVPVQRYYVPSCEEMEVCD
                     PYGQCWLEQFCR"
     gene            6054..7100
                     /locus_tag="U6309_001008"
     CDS             6054..7100
                     /locus_tag="U6309_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEN5504461.1"
                     /translation="MSETIGYAAHKAGAPLKPFTFERRPVGAHDVAIDILFCGICHSD
                     LHQVNNDWAGAFYPMVPGHEIIGRVKEVGHQVHRFKAGDLVGVGCMVDSCRQCTYCHQ
                     NEEQYCKSGATYTYNSQDNHLGGVTYGGYSKHIVVTEDFVLKIDESLDPAAAAPLLCA
                     GITTYSPLKRAKVGKMSKVGIVGLGGLGHMAVKLARAMGAYVTVFTTSINKIKAGKDL
                     GAHDVVLSTDSNVMQTLANQYDFILDTVSAKHDLGQYLNLLKVDGQLTQVGLPSDPLE
                     VTMFPVILKRLSINGSLIGGIKETQEVLDFCARHEITADIELIKMHEVNSAYERLLKG
                     DVKYRFVIDMSTLD"
     gene            complement(7154..8473)
                     /locus_tag="U6309_001009"
     CDS             complement(7154..8473)
                     /locus_tag="U6309_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN5504462.1"
                     /translation="MYNILLIFIAFLLVLLNAFFVSAEFGMVKLRATRIQAIKENYGL
                     RGRILEQVHQNLDAYLSACQLGITLASLGLGWIGEPAFAELLGPLFVALGISSVHIVT
                     ISSFFIAFFLISFLHIVIGELMPKSIAIRQSELISVWTAVPLYWFYWIMYPAIWFLNT
                     CSNFFLKLSGMDIKNEYEGFYSSEELKLILSGQHLQSELKEDEREILEHTLDLAELNV
                     TEVMRPEDEMIIIDINEPFETIKDKILKYRYSRYPVYDSGTKEMIGVIHVKDLFADLL
                     LQKNINTLKPYVRPVLKVSHKLPAMDLLRKFRAGMPHFALVYKHKSSLLGFVTLDNLL
                     HILIGRIKDEFHKTHEDWVKNLDGTITTKGDCSLYSLERALDCDITLSPDEEELNTLY
                     GLIITRHGKLPNEGEIIQFVEFDALIEKVQDSFITKIKVFPKIKNQE"
     gene            9127..10422
                     /locus_tag="U6309_001010"
     CDS             9127..10422
                     /locus_tag="U6309_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5504463.1"
                     /translation="MNQNCFKPKNNFVSLAMWTLPLSFFTFQFLLRLWPGLTMQDIMA
                     QFSIDAASFGLIAAFYYYGYAGMQIPVALLLDKFGARFVVFAFAMLCGIGTLVFIGTN
                     NFYLALSGRFLIGVGSAAGFLGVSKVVSEWFSHSQYSRMIGFSFSVGLLGAIYGGKPL
                     AHLIQNFSGQQVGLALAVFSILLGCCALFILRSPPNNTHSVTKTMNLSHCSFLFSSWI
                     IWALALANLLMVGALEGFADVWGVPYLIAKYALNKTDAAFLISFIFMGMIFGGPLLAF
                     LSQKLGNYFVIALCGAGLAFLFLILLYTSTLHWWQLGTLFFAIGLLCCYQVIVFAAGS
                     TLVPYEGLGVTIAFLNCINMLGGSFFHTLIGKLMNGSWSGQMSANGLKLYEIESYQWA
                     LSLIPFCALCGSLLIVLIHIKTRAYAAPGFMKKTSLTGV"
     gene            10425..11069
                     /locus_tag="U6309_001011"
     CDS             10425..11069
                     /locus_tag="U6309_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEN5504464.1"
                     /translation="MEKIFWSNPYQHTLDTKIISINGNEILLEKTIAFSFSGGQESDR
                     AFINGLEVLSSRIDGNLIYYTLPDDHNLAPNDVITMTIDWPRRYRLMRLHFAAELILE
                     LVTRTSMLEKVGAHISESKARIDFKANFNISTIFSQLLDEYNAIIRQDLVIHKGYHDV
                     TNQRRFWKIDGFAEVPCGGTHVHSTSEVGFIRLKRSHPGKAIERIEITLMDDSR"
     gene            11316..12230
                     /locus_tag="U6309_001012"
     CDS             11316..12230
                     /locus_tag="U6309_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEN5504465.1"
                     /translation="MELPNHFSTVYVSKPEFIETLSEELGEVSNKIGSLVFSPHQKLD
                     VCFALDVWLEPEIVTFQSISEAAKILRQAGKFWYLHPVTEIRRSRLIEEQLRKCPSLM
                     SHFPVTNDIPSIGVFSLLDRNTLLFSAKRLKKWPDGQCYFIEDKANPPNRAYLKLWEA
                     LFLLNKYPCPGETVLDLGASPGGWTYVMQSLGTQVTSVDKAELDTKIMALPRVSFLKQ
                     SAFALDPEQFTESYDWVLSDVACYPDRAYTLIKKWIASKKARQLIFTIKLQGQTDLNT
                     IKRFQSIPNSKVINLFYNKHEVTFFYPV"
     gene            complement(12303..13256)
                     /gene="epmA"
                     /locus_tag="U6309_001013"
     CDS             complement(12303..13256)
                     /gene="epmA"
                     /locus_tag="U6309_001013"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HEN5504466.1"
                     /translation="MSDNSWLPSASIQLLRARAELIGKIRQFFTERQYLEVETPVMAR
                     HGVTDVYLSNIKAIFRGETCYLQTSPEYHMKRLLAAGSGPIFQLARVFRDDELGRWHN
                     PEFTLLEWYQLDINHHILMDEVDLLLQIILKSRPMIRKTYQQAFEEACGIDPFSTSIE
                     QFTKILAQHGLDGVLSSHEQDRDQYLFLLMSHVVEPFLAEESTPVAVYHFPASQAALA
                     QVKEGVAERFEVYYQGVELANGFHELTDVEAQKKRFMLDQQHRKQKGLTAMEADAYLL
                     EALEHGLPPCSGVALGVDRLLALALKQPSIEKILSFDFSRS"
     gene            complement(13259..13594)
                     /locus_tag="U6309_001014"
     CDS             complement(13259..13594)
                     /locus_tag="U6309_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEN5504467.1"
                     /translation="MFGFKKKQSNESIKEGVITALRGVFDPEIPVNIYDLGLIYDIAV
                     NDEGHVHIQMTLTTPGCPVAQTFPGTVEQAVNQVEGVSDCTVELVWEPPWSQDRMTEA
                     ARLELGIFY"
     gene            complement(13666..14115)
                     /locus_tag="U6309_001015"
     CDS             complement(13666..14115)
                     /locus_tag="U6309_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEN5504468.1"
                     /translation="MSMELRELYQEIIIDHNRNPRNHHVMEDATTEAKGFNPLCGDKL
                     TVYLKLQGDLIRDVSFVGCGCAISQASASLMTDALKGKSIKEAHELFHRVHRMLTQEE
                     EDSLVSMDKLTVLAGVKAFPARVKCATLAWHTLEAALNKETEVVKTE"
     gene            complement(14112..15356)
                     /locus_tag="U6309_001016"
     CDS             complement(14112..15356)
                     /locus_tag="U6309_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEN5504469.1"
                     /translation="MSTNPSLMTSFDVNKIRKDFPVLHQKINEYDLVYFDNAATTQKP
                     KAVIDAIAQFYEKDNSNVHRGVHALSVRATEMYEAARAKVKRFINARSPRECIFVRGT
                     TEAINLVAQSLVAPRILPDEEILITHMEHHSNIVPWQMVCKKMGCKLQVAPISLNGEV
                     ILEEFERKLNENTKMVAINYASNSLGTINPVKTMIKMAHEVGAKVLLDGAQATAHLIV
                     DVQDLDCDFYAFSGHKMYGPTGIGVLWGKEELLNSMTPYQGGGEMINSVSFEATEYAA
                     IPHKFEAGTPNIAGAIGLAAAIDYIWSLDLDAIAEYETQLLNYATKAIEAVKGYNIIG
                     TAANKVPIISFVHGKIHAHDIGTILDSEGIAIRSGHHCTMPLMDFYDVAATSRISMSF
                     YNTFKEIDYCMEALQRVKEVFA"
     gene            complement(15346..16632)
                     /gene="sufD"
                     /locus_tag="U6309_001017"
     CDS             complement(15346..16632)
                     /gene="sufD"
                     /locus_tag="U6309_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEN5504470.1"
                     /translation="MSDILDYYQQQAQSGVSQIPWLAQLQSKALTDLKRRGFPTRYDE
                     EWKYTLVDSLLQQHFVLPDPTVKPALKAVFYDSPVKNLFLIQNGFISQEQEWIKNLPE
                     GVLILPLSLALIHYADLVKPYLGKIVEQEHGFHYLNTAMLQCGVFIYIPAGICLEEPI
                     VLKHIQDQTNQAMYLRHLILMEKESQATLIEEYQGEENICYFTNSITEVFLGAGAKLN
                     HYKIQKESKSAYHLGHLSIRQSAASQFSSHSLSLGGKLVRSDISVFMQEEQAQCLMNG
                     IYAPGDGQHIDHHTVVHHLVPNCRSDQDYKGILTGHSRAVFNGKVIVAKNAQHTDAKQ
                     QNKNLLLSANAEVDTKPQLEIFADDVLCSHGATVGQLDEEAIFYLATRGISRDEASHY
                     LIQAFANDNLRLIPNRDLADWMGNLLTQQLGYANEY"
     gene            complement(16629..17381)
                     /gene="sufC"
                     /locus_tag="U6309_001018"
     CDS             complement(16629..17381)
                     /gene="sufC"
                     /locus_tag="U6309_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEN5504471.1"
                     /translation="MLNIKELNVAVNGQSILKGINLEVKPGEVHAIMGPNGSGKSTLS
                     KVLAGHPSYEITRGDISYLGQDLLPMSPEERARAGIFMSFQYPVEIPGVTNINFLKAS
                     VNAVRKGQGRNTLDAIEFLSFIREKCQLLDMDESFLYRSINEGFSGGEKKRNEILQMA
                     ALEPRLAILDETDSGLDIDALRIISQGVNAMRSPERSIILVTHYQRLLDYIEPDFIHV
                     LANGRIITSGDKSLALELEKKGYSWLEEVEQL"
     gene            complement(17383..18831)
                     /gene="sufB"
                     /locus_tag="U6309_001019"
     CDS             complement(17383..18831)
                     /gene="sufB"
                     /locus_tag="U6309_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEN5504472.1"
                     /translation="MAKSSEQLNSLLDREYQHGFITDIEVETFPPGLDEDVIRRLSAI
                     KGEPEFLLEWRLKAFRHWLTMPHPEWSSVHYLPIDYQSISYYSAPKSKKDAPKSLDEV
                     DPELLKTYEKLGIPLREQEMLAGVAVDAVFDSVSVATTFKAKLAEKGVIFCPLSEAVH
                     QYPDLVKKYLGSVVPYRDNFYAALNSAVFSDGSFVYVPKGVRCPMELSTYFRINAAST
                     GQFERTLIVADVDSYVSYLEGCTAPMRDENQLHAAVVELIALDGAQIKYSTVQNWYPG
                     DKEGKGGIYNFVTKRGACRGKKSKISWTQIETGSAITWKYPSVILQGDDSVGEFYSVA
                     LTNNFQQADTGTKMIHLGKNTRSTIISKGISAGCAHNAYRGLVRIAPTATNARNYTQC
                     DSMLMGSQCSAHTFPYIEVKNPTAQVEHEATTSKISEEQLFYCQQRGIDTEDAVSMIV
                     NGFCKQVLKELPMEFAVEATKLLGISLEGAVG"
     gene            complement(18824..19285)
                     /locus_tag="U6309_001020"
     CDS             complement(18824..19285)
                     /locus_tag="U6309_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HEN5504473.1"
                     /translation="MLRVSKLADYGTVVMVYLAKHSQELCNARDIALHTHLTVPTVSK
                     ILKRLTSAGLLTSVRGVSGGYRLQRPAAEISVSQIIFALEEHRGFTECSMQPNDCFLQ
                     GVCTIQGNWRLISHAIETALDSVSLEALAKPTLPTRDVERVRQLASGVNRG"
     gene            19726..21468
                     /gene="phaC"
                     /locus_tag="U6309_001021"
     CDS             19726..21468
                     /gene="phaC"
                     /locus_tag="U6309_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEN5504474.1"
                     /translation="MTHDTELSELMQAVAKKSLQIMTDFKEKPIPISSLVSQYIDLTE
                     HFQNLIAVILKNPEKVWQMQLNYLEDALSLAQAQFNYWLEGKPLPINDQRFNGEDWIN
                     NPFFNLLSQHYLLANEHMNSLLENMEYGDENLAKRVRFFTRQYLDALSPANFIHTNPQ
                     LMAETLQSHGKNLLRGLHNLLSDVEAGSSRLIIKMTDTEAFKIGENLATTPGKVIFRN
                     SMMELIQYCPRTTKVKSIPLLIVPPWINKYYILDLSPHNSLIRWLVEQGITVFIISWV
                     NPDETYANKSFYDYLNEGPREAIAVIQKQLRVKQVNTLGFCIGGTLLASLLAYNKATK
                     DHSIRSATFLAAMIDFSDPGDIAVFIDEQQINKLEEEMKSKGYLAGKFMASSFNSLRA
                     NDLIWSFFIKNYLRGKSPVPFDILYWNADSTNMPATMHSQYLRWMYLHNNLIKPGKIR
                     LNHIPIDVTNIDIPTFFLSTQKDHIAPWKTTYKGFELMKGPKRFVLGGSGHIAGIINP
                     PTQQKYGYRTNNSMDLSAEQWFEKSKEHSGSWWPEWLNWLKLHSGRLINSPDINHLPF
                     APIMDAPGSYVLKK"
     gene            21517..21900
                     /locus_tag="U6309_001022"
     CDS             21517..21900
                     /locus_tag="U6309_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504475.1"
                     /translation="MRKKNRILLASLFLLGSLNAFAAQDLTVVNTSDDLGYVTIGSYS
                     CELTSSCAIPPDNSIHINSDILEAACANTSADCEIVFGVDSIKARIATGSFSLNEGIQ
                     YIAQSRWLNYSAKRLSKTVAEIYKR"
     gene            complement(21987..22406)
                     /locus_tag="U6309_001023"
     CDS             complement(21987..22406)
                     /locus_tag="U6309_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEN5504476.1"
                     /translation="MKLIWIVLALILALVVILYLFPQINFREMPKGLVEGQLPDDKPN
                     WVSSLVNESDSHYIAPLKVESMPQLVACIERKIPELKIQKQEASFLVGYRQSPFFHFV
                     DWLCIHADGNVISSATMGYSDFGKNRELVEKIRSLCL"
     gene            complement(22407..>23855)
                     /locus_tag="U6309_001024"
     CDS             complement(22407..>23855)
                     /locus_tag="U6309_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEN5504477.1"
                     /translation="FLARCFILGRQIKKQTAVGETVGVMMPTTIAGMVTFFALQAYRR
                     IPAMLNFSMGFYNLYSACNTARIKSIYTARQFIETAKLEPLVEEFQQAGIRIHYLEDF
                     KSTIHLGNKLSGIFKGFFPTLSYRFLGDKISSDTTGIILFTSGSEGKPKGVALSHANI
                     LANCWQMTSRVDFTPRDVLFNSLPIFHCFGLTAGSVLPLVNGLNCFFYPSPLHYKVIP
                     GLIYQTRATILFGTDTFLTGYARAAGKHDFNSVRYIFAGAEKVKPETIRYWSEAFGAR
                     IYEGYGATEASPVISLNCPLASVPGSVGMILPFMDFKVEPVEGIVQGGRLKLCGPNIM
                     LGYLDEDKPGVIIEPEDGWHDTGDIVTVNADGFITIAGRAKRFAKIAGEMVSLTAVEG
                     IAASIWPELLHAAVVQKSPRKGEQIFLFTEAEYADKVSFIKKVKERGQSELLVPHMIY
                     PACQIPVLPSGKIDYITLEKQFLEQKETMETV"
BASE COUNT         7414 a         4979 c         4377 g         7085 t
ORIGIN      
        1 aatcgaacag cgatgagtaa atctgtggaa ttaagtttaa gatttatgcc tgaattggta
       61 caaatttcca ggcaatctta gcataggaaa gatgtaatgt attgaaaatt aattattaaa
      121 ttcccaggcg ggattaaaat ttatccctta ggggataagt accttctttt attggcgtat
      181 taaacgaata ttttgcccat caaaagcaca aggttctgtt gacctgattg ggttaatatc
      241 cagacctccc cttcttgtat atcgtccata aacagtcaat gactcaggtt ggcaaaatcg
      301 cataatgtct gcaaatatcc gttcaatgca ctgctcatga aattcattat gatttctaaa
      361 tgaaatcagg tatttaagca acccttcatg atttatcttt ttacctttat aaataatttg
      421 aacgcttccc caatccggtt ggttggtaac caggcagttt gatttaagta aatcagaata
      481 caatacctct tcaactaatt cagacgaggt cgaaagaaaa tcaggcatta catgatatac
      541 agagcattca atatcaagct tatcaaggca aacaccatcg aatgcagcat agatagtttg
      601 attagggata tccgacaagg ggtacacatt aaccgccact tcagaaccaa tgcagtaact
      661 taaatctttg ctgatagttt gcacaacggt ttccacatca gggaaagtac tattattcaa
      721 cgagttaaaa tagagtttta atgactttga ttcaataata cacggggaat gacaatcata
      781 agaaataaca gccatcgcaa caacaggttt acctttggag tttaaccagg agacctcata
      841 atgattccag caatcaaaac catagaaagt ggtggagttg ggatccaaat taatttcctg
      901 gcgttttggg gctctgggga taggatacag ccttttagga ttatagtagc tgtcataatt
      961 tgcagtttgc cccagctccg attgatttgc tatattctga taaatacttt caagttcctt
     1021 attcattatg gatgcctttt gtaactctaa aaaacaaagt gattataatc tatcatgtta
     1081 cctaagggtc aattttttct taaaaaaaag gaaatagcct atatttaatt aaagatactg
     1141 aaattagcat ctaaaggagt gcatttaccc atgattaatc aacagataga agtatttttg
     1201 aaatctcctg cttttgccgt agttggcgcc tcttccaata gggaaaaata cggcaacaaa
     1261 gtattgcgat gctatcttcg gaatggcaaa aaagtctatc ctgtaaatcc acatgaaacg
     1321 ctgattgaaa gcttgcctgt tattcattca gtaaacgatt tacctgatga tgtacacagt
     1381 atttctatta tcacccctcc atccattact gaagaagtcg tcaaggaagc agtcaaaaaa
     1441 ggaatccaac atatatggat gcagcctgga gcagagagtg actcggccat tcaatatggc
     1501 ttggatcaca agcttaatgt catttctggg ggaccttgta tattggttgt catggggtat
     1561 gaagagaatt aaggaaatac aaaaagctat tttgtagtcc aaaatagctt ttttgatttt
     1621 gtataatccc agccgattat ttagaacgcc tgaattgctt taatgattca ttgaccacac
     1681 tttcaagagt tctatgttct gcatattttt ctcttaaaaa gctggcatca ctccctttat
     1741 gagttatttc ataaatatgc tccaaagcca tagtactatt tagctgatta gcataaggct
     1801 ttaatcggcg aagtgttgtt aaaatatctt ctcgaagcaa aatttgctca tatgttttgg
     1861 gatgcaccag agtgccatcc agaccaaatc ggcatgcttg aaaacgattg taattgtaca
     1921 caagataatc atcttcgtga ggcagaggtt ctttattttc caataaataa ctgcaaagcg
     1981 cttgcatata acatgctaaa gcggctgctc gctcaaccgt aaggggacta tcacacaccc
     2041 gcatttcaat tgtcccaaat tcaggtttag gtcttaaatc ccaataaaaa tccttcatac
     2101 ttttcactat gccggtatgc tccatttttt cgaaataacc gagtgagaat tcatcccagt
     2161 ttaatacaaa gggagccctt ccacttaatg ggaaggcaaa taccgaattc aatcgagcag
     2221 agttatacaa ggtatcctta ctctgaacga agggagatga agcagacaat gcaataaaat
     2281 gagggatata acgatttaaa gaatgaagta aaaaaagcgc ttgattaccg tcctcacaac
     2341 caatatgtat atgttgccca aaaatagtaa attgcttggt tagatagcca tataattcag
     2401 acacctctat aaacctggtt ttattaaaaa ttcgctgatc agaccacatt tgaaatggat
     2461 gggttccccc gccacaaata ccaatattta atcggtctcc aactgtcacc agggcatcgc
     2521 gtatatgaaa cagttgtttc agtagttgat catattcttc atgcacatct gtcgctattt
     2581 ctaacatact ctctgtgatt tctggcgtaa aactgccggg aaaagacgat cgtcccaaga
     2641 gctccaatat atcagggcta gcggcagtca agtcaaaatt acttaagctg attaattgta
     2701 attccagctc aacccccata gtcagtaaat tagatgtttt gaatggttgt aaaggcattt
     2761 caaaattcct tgattgcaga gatctcgttc gctttaatta aagcacgttg aaccattatt
     2821 ggacccaata tttccaaagt cagagcaacc atagccagcg gggccagtgt atctaccaaa
     2881 ttaataccaa tatatcggct ctgttctaaa atcaaaatga caaaaacaga gatgggggtc
     2941 atagccaaac cagtcaagat acctttttta agcgaaatcc cactaatatg ggcaaataaa
     3001 ctaatcccga taattttagc tacgaacctt acagttataa ttgataatcc tatccataca
     3061 ccagttacta ccctttgcca ttctatggtt gctgcaataa atacaaacaa aagtaccgat
     3121 aacaaatcac ctaaggtgcc aaatcctggc tgtgaaggag aaaaaaccat tcgcccatga
     3181 cgggaaacca acccaaaggt taatgtggct aaaaccgggg acaattttaa attatgggtt
     3241 aaaataacca gcagtatcac agcgcaggca aatgccaacg tattgtcttg acaggtgctt
     3301 ttaactgttt taagcaaaat gggcattatc actccaaaaa taatccccca aatagttgaa
     3361 atggctgtca ccgttaagct ggcataagct gcttcccaaa gatttcctga agttctgaaa
     3421 acaaccaagc caatgatgac tttgaataaa aatactgcca gcacacaatt gatcacggag
     3481 agatgtaaaa cacgttctgt cacttggcct gaactacgtt gttcattaat caccctgact
     3541 acagtggctg gtgaagtcgc catggacaac gaagccaata aaaggcaggt cgaagtttgt
     3601 aacccaaagg atttcactaa aatataaacc aggcaaaagg ttaaagtcgc ttcggtaata
     3661 ctggatgctg caacccaggg gttataactt aaccagcgta aattaattct atagcctgat
     3721 tcaaacaaga ttagcccaaa accaatattg gctaaaagta aaatagatgg tggttgaata
     3781 gacggtaata atcctatttg agttgatgct agtgaaaatc ctacaatcgc atagatactg
     3841 atatgaggca attttgccca tcgatatcca agttcccctg caaaccaagc tatcaataaa
     3901 ctgataggcc aagccaagtc ggttataatt tttccaacat caatcatcca tacctcctat
     3961 gaaaatccga ccttacttta taacacacat cgatttgttt tgcagaattg aagtattttt
     4021 aagacaatat aaaataatca ggacttacga aaaatcctaa ggtcgaggca aaaaatgttt
     4081 ttaatgaatg agtttagata aaataaatgg ctgaattaaa aactttttta acaaagagat
     4141 tgaggttttt cgtgagcact gatagttttt tgtccaatta ggaaaatcat tgctactaac
     4201 cacattaacg agaaccaaaa agaaatctca acaacttctt attttaatag gcttcttatt
     4261 agggatgtca ccagtcatgg ctttagaaat tattacaata gaaaaaaaag aaaactacca
     4321 tatgctaaaa actcgggctg aaaatattca atttcccttg aatcgagaag ataaagattt
     4381 aatagaagcg atgaaaaaaa aattatacgc attgggaggg gttggtttgg cagcaccaca
     4441 agtcaattcc gccaaacaaa ttatcgcagt ttacatccct gaagaagctc agcttttacg
     4501 tgataatgcg aaaatctttc caatgcacat cctaatcaat ccaaattacg agcccctgcc
     4561 ccacactcgt attattagcg actttgaagg ttgttattct gtatccagta aagcagggaa
     4621 agtgccaaga tatgatgaaa ttcagctaaa gtactatgat gaagaaggga atgttcacca
     4681 acaaatcgaa aatggctttt acgcccgtgt tttgcaacat gaaattgatc atctgaatgg
     4741 cgtattaatc accgatcgtt taactcccga ttgtgtacaa gggactattg aagaaatgat
     4801 gactattcgc cgtgctgaat tgcctgtaga aaaacgaaaa ttatttgatc aaatcatggc
     4861 aaaaaaactt aaaaaataag gtcgagttcc caaattatct tttatgaatt gacttcaatg
     4921 ataaactgcc ctgggtaatt tttttgcttg ttcaacccag gtaacaactt gagataatcc
     4981 aggtacatgt tgcgccaaat gacattgctc atttttctct tttaatgagc tataaagttg
     5041 ttcaggattg caatgtgcta attctgggat tgagtcaata ccagcctgtt ccagaagctc
     5101 agcatattct tcattaatgc catttactcg cgcaagatca gcttgattgg tccatttcaa
     5161 aatcaattta tgactgattc ccgtttgctc ggctactcta aaacgcccac ggcgaccact
     5221 acaaactctt aataaatcgt cctggctttc aatccctact cgctttaaca tttcagcata
     5281 ttttgggcct acgccttgca gctcaattaa cttgtgcatt ttatttccag tcttctacta
     5341 cgatattaca tatataaaat tatagaatat aatgattaat tattaaccaa aaacaaatgt
     5401 tgaaaaaatt taggcctcac gtattaaaaa aaatcaattc atttggcaaa atgaaacaat
     5461 ttcattatga taattacttt cctttcagtc cctggtgagg tagaaaatgc gcaagcacaa
     5521 gaacaaaatg gtcaggatat ttttattgac tttaggacta ttttccagca ccaccattaa
     5581 tgcttggtat ggctcaggga taaccattgg tgttgccaga gcgaatgttc actattacgg
     5641 agatgatatc cgagtatatg acccgtgggg ctatggatac tacgatagaa attattacta
     5701 taatggagta agaaacatta tcgttcctaa tgtaattgtg ccgaatgtag tgatcaatgt
     5761 accagttcaa aggtattatg taccctcatg tgaggaaatg gaagtttgtg atccttatgg
     5821 acaatgttgg ttagagcaat tttgcagata aattaataac tcaatcaact aatgttttgg
     5881 gactcagccc attatgggat gagatattag caggacttac aaaaatccca agttcaagac
     5941 aaacaatgct tttaatgagg gagtttagat aaactaaaca accgaattaa aaacattttc
     6001 taacaaagag attgggaggg tatgtcctga ttataatttt ttaaggacaa aaaatgtcag
     6061 aaactattgg ttatgccgcc cacaaagcgg gagccccttt aaaacctttc acttttgaac
     6121 gccgccctgt tggtgcacac gacgtagcaa ttgatattct attttgtggt atctgtcatt
     6181 ccgatttgca tcaagtcaat aatgattggg caggagcgtt ctatcctatg gttcctggac
     6241 atgaaatcat tggacgagtc aaggaggtag gacatcaagt ccaccggttc aaagcaggag
     6301 atttagtggg cgttggctgt atggttgact cctgccgtca atgcacctat tgccatcaga
     6361 atgaagaaca atattgtaaa tccggggcaa cctacaccta taatagtcaa gacaatcatc
     6421 tgggaggggt gacctacggg ggatattcca agcatattgt cgtcacggaa gattttgtat
     6481 taaaaattga tgaatcactt gatcctgccg ccgctgcacc attgctctgt gccggaataa
     6541 ccacttactc cccattgaaa agggctaaag taggtaaaat gagtaaagta ggaatcgttg
     6601 gcctgggtgg cttaggacat atggctgtca aattagcccg tgccatgggg gcttatgtga
     6661 ctgtatttac cacttctata aacaagataa aagcaggtaa agatttggga gctcatgatg
     6721 ttgtcttgtc tactgacagc aatgtcatgc aaacactagc caatcaatat gattttattc
     6781 ttgatactgt ttctgccaaa catgatcttg gccaatattt aaaccttctt aaagtggatg
     6841 gacaactaac ccaggttggg cttccatcag atccacttga agttaccatg tttcctgtta
     6901 ttttaaaaag actgagcatt aacggctcat tgattggcgg cattaaagag acacaagaag
     6961 ttttggattt ttgcgccagg catgagatca ccgcagacat tgaattaata aaaatgcatg
     7021 aagtaaattc tgcctatgaa agactgctaa aaggagatgt gaaatatcga tttgttatcg
     7081 atatgtcaac cttggattaa atgaaccagg tataaaaggc ttccagttgg ccttttatac
     7141 cacggagcaa atattattcc tggtttttga tcttggggaa aactttaatt ttggttataa
     7201 aagaatcctg taccttttca atcaaggcat caaattcaac aaattgaatt atttcacctt
     7261 cattgggcaa ttttccgtgg cgcgtgataa taagtccata tagtgtattc agttcttctt
     7321 catctggcga taaagtaata tcgcaatcaa gagccctttc taaagaataa agggagcaat
     7381 ctccttttgt cgttatagtt ccgtccaaat tttttaccca atcttcatgt gttttatgaa
     7441 actcgtcttt aatccgccca atcaggatat ggagcaaatt atccagagtt acaaatccaa
     7501 gtaaactcga tttgtgtttg tataccaaag caaaatgagg catacctgct cgaaatttac
     7561 gcagtaaatc catagcaggt aatttatgac tgactttaag tactggccgt acataaggct
     7621 ttaatgtatt gatatttttc tggagcaaca aatcggcaaa taaatcctta acatggatga
     7681 caccaatcat ttccttagtc ccggaatcat agacaggata acgactataa cgatatttta
     7741 aaattttatc tttaatcgtt tcgaatggct cattgatatc tattatgatc atttcatctt
     7801 cagggcgcat tacctcagtg acatttaact cagccaaatc gagagtatgc tcgagaattt
     7861 cacgttcatc ctcttttaat tccgattgca gatgctgccc gcttaaaatt aatttaagtt
     7921 cctcacttga ataaaaacct tcgtattcgt tttttatatc catgccggac agtttgagaa
     7981 aaaaattgga acaggtattt aaaaaccaaa tagcaggata cattatccag taaaaccaat
     8041 acaacgggac tgcagtccat actgaaatca attcagattg ccgaatagca atggacttgg
     8101 gcatgagctc accgattaca atatgtagaa aagaaataag aaaaaatgca ataaaaaaag
     8161 aagatatagt aacaatatga accgatgaaa tacccaaagc aacaaataat ggtcccaata
     8221 actccgcaaa agcaggctct ccaatccaac ccaatcctaa cgaagcaaga gtaatgccta
     8281 actgacatgc tgataaatac gcatccagat tttgatggac ttgctcaaga atgcgacctc
     8341 ttagcccgta attttctttg atggcttgaa ttctcgtagc cctgagttta accatcccaa
     8401 attcggcgga tacaaaaaat gcgttgagaa gcaccagcag gaatgctata aaaattaaca
     8461 gaatgttata cattaaaaat agtccggttt atgatttaat ttcacagaac ttacgaaaaa
     8521 ccccaatctc ttcgttaaaa atatttataa ttcggtcatt tggtttatat aaaatccctc
     8581 attaaaaata ttttttgcct tgaccttgtg gtttttcgta agtcctgttt tctattcagt
     8641 cgtaccttca ctaaaaacat tcactctcta caaatgtgat cgaatccttt atgttaatag
     8701 aatatcgccg tcctggcgag tttaaaataa ctaagccaga actcaaagat acaataatta
     8761 ttatccggtt taatgacaaa ttatttaatc aaagcaaccg tatataaatg acaccaaata
     8821 cccgtaaatg gcggccccct attgacaaat agaagcacaa tttgttaatt tgattcatta
     8881 aaagacaaaa ttaactcaac ctgaaaagat tatgaatcgt ccaaacagca aaacatgcca
     8941 agacttttgt gcaaattctt atgccaatgt tttgctttgc ggcgcttccc agtctataga
     9001 taatctttca atcattatta ttatcttgcc ctctttcccc tagagcggtc agctgttgct
     9061 aattctctgc ctctctaggg aggcatccaa agagttagca ccaactgatt tcaactcgag
     9121 ggcatcatga atcaaaattg tttcaaacca aagaataatt tcgtctcatt ggcgatgtgg
     9181 acattacctt tatctttttt tacttttcaa ttcttattac gtctttggcc tggattaacc
     9241 atgcaagaca ttatggcgca attttcaatt gatgccgcaa gctttggcct tatcgctgca
     9301 ttctattatt atggatacgc aggaatgcaa atccctgtag ccttactgct ggataaattt
     9361 ggagcccgct tcgttgtatt tgcctttgca atgttgtgtg gtattggaac tttagttttc
     9421 ataggtacca ataattttta cttggctctg tcaggcaggt ttttgatagg tgtaggctcc
     9481 gcagctggtt ttcttggcgt atccaaagtc gtatcagagt ggttcagcca ttcacagtac
     9541 tccagaatga tcggtttctc ctttagcgta ggcttgttgg gggcgatcta tggaggaaag
     9601 ccgcttgccc acttgataca gaattttagt ggacaacaag ttggtttggc tttggcggtt
     9661 ttctccatcc tcttaggatg ttgcgcgctt tttatcttgc gctctccccc aaataatact
     9721 cactcagtaa caaaaacaat gaatttgtct cactgttcct ttttattttc atcgtggatt
     9781 atctgggctc ttgctcttgc taatttatta atggtcggtg ccctggaggg attcgcggac
     9841 gtatggggag taccttatct tatcgccaaa tacgcgctga acaaaactga tgctgccttt
     9901 ctcatctcgt ttatctttat gggaatgata tttggcggtc ctttactcgc tttcctcagt
     9961 caaaaattag gaaactattt tgtaatagcc ctctgtggtg ctggtttggc cttcttgttc
    10021 ctaatccttc tttacacctc tacccttcac tggtggcagc tgggcaccct cttctttgca
    10081 atcggcctgc tgtgttgcta tcaagttatt gtattcgccg caggttctac tttggttcct
    10141 tatgaaggtt taggcgtcac aatcgccttc ttgaattgca tcaatatgtt aggcgggtct
    10201 ttttttcata ctttgattgg taaattaatg aatggcagtt ggagcgggca aatgagcgct
    10261 aatggactta aattatatga aatcgaatcg tatcaatggg ctttgtcact cattccattc
    10321 tgtgccttat gtggcagttt gcttattgta ttaattcata tcaaaacaag agcttatgca
    10381 gctccaggat ttatgaaaaa gacttcattg acaggagtgt agctatggaa aaaatttttt
    10441 ggtctaatcc ctaccagcat acactcgata ccaagataat ctcgattaat ggcaacgaga
    10501 ttctattaga aaaaaccatc gcgttttcat tttcaggagg acaggaaagc gatagagcat
    10561 tcatcaatgg ccttgaagta ctcagttcac gaatagatgg aaatcttatt tattataccc
    10621 tgcctgatga ccataatctt gcgcccaatg atgtaatcac catgactata gattggcctc
    10681 gaagataccg gctgatgcgt cttcattttg cagcagaact gattctggaa cttgtaacca
    10741 gaaccagtat gcttgaaaaa gtaggagctc acatctcgga atccaaggct cgaatagact
    10801 ttaaggcgaa ttttaatatc tctactattt ttagccagct gcttgatgaa tataatgcca
    10861 tcattagaca agatcttgtt atccataaag gctaccatga tgtgacaaat caacgtcggt
    10921 tttggaaaat tgacggtttt gctgaagtcc cttgtggggg aacacatgtt cattccactt
    10981 cagaagtggg ttttattcgt ctcaaacgct cgcatcctgg caaagctatt gaacgcattg
    11041 aaataaccct gatggatgat agtagataat gcacctcaca gataagctga ttgttttgct
    11101 ttggggcaag gtgagaatgt tatggggcac ggagtttata tacattgaat gagcgttgct
    11161 gaaacgttct gatcgtgaca gtaaaataaa taaagcgggt tattcaggaa gccaggtcaa
    11221 acgctaatcg agacaaaaat atagaaaaag aatacaaaat cgcctaaatt acagcatttg
    11281 actttgtcgc cattaacctg cgagcgttat ttatcatgga actgcccaat catttctcta
    11341 ctgtctatgt gtcaaaacca gaatttatag aaacactttc tgaggagtta ggcgaggtat
    11401 ccaataaaat aggctctctc gttttttctc ctcatcagaa gcttgatgtt tgttttgcac
    11461 tggatgtatg gcttgaacct gagatagtaa cctttcaatc gatttcagaa gcagcaaaaa
    11521 tattgaggca agccggaaaa ttctggtatt tacaccctgt tactgaaatt cgccggtcgc
    11581 gcttgattga ggaacaactt cgcaagtgcc catcactaat gtcccatttt ccagttacaa
    11641 atgacattcc atcaataggt gtatttagtc ttcttgatag aaacactcta ctctttagcg
    11701 ccaaacgact aaaaaaatgg ccggatggac aatgttattt tattgaagac aaagccaatc
    11761 ccccaaaccg ggcgtattta aaattatggg aagccttgtt cttgttaaat aaataccctt
    11821 gccccggtga aacagtttta gatttaggcg catccccggg aggttggaca tatgtgatgc
    11881 aatcactcgg aacccaagtc acttcggtcg acaaagcaga actggataca aaaatcatgg
    11941 cgcttcccag agtcagtttt ttaaagcaaa gcgcctttgc cctagatccc gaacaattca
    12001 cagagtctta tgactgggta ctttccgatg ttgcctgcta tcctgacaga gcctacaccc
    12061 tcatcaaaaa atggatagca tcaaaaaaag caagacagtt gatttttacc atcaaactac
    12121 agggacaaac tgacttaaac acaatcaaac gatttcaatc catacctaat tccaaagtaa
    12181 tcaatctgtt ttataacaaa catgaagtca cgttttttta tccagtataa acataaccag
    12241 gatttagtga ttatgtctgc aaaaatatca taatgacaag ctaatactta attttctatg
    12301 acttaagacc ttgaaaaatc aaatgaaaga attttttcta tgcttggttg ttttaaggcg
    12361 agcgccaaca atcgatcaac cccaagagcc accccgctgc aaggtggtaa cccatgttcc
    12421 agggcttcca aaaggtaggc atcggcttcc attgccgtca atcccttttg ctttctatgt
    12481 tgctgatcca gcataaaacg ctttttttgc gcctcgacat cagtcaactc atgaaaacca
    12541 ttagccaatt caactccctg atagtacact tcaaagcgct cagccacgcc ctcttttact
    12601 tgtgccaagg cagcctggga ggcagggaag tgatacacag caacgggggt tgattcttct
    12661 gccaaaaaag gctccacgac atgactcatt aataaaaaca gatattgatc cctgtcttgt
    12721 tcgtgagaag ataagacacc atccaaacca tgctgtgcta atatcttggt gaattgttct
    12781 atggatgtag aaaaagggtc aataccgcaa gcctcttcaa aagcctgttg ataggttttt
    12841 ctgatcatag gccgggattt aagaataatt tgtaataata aatccacttc atccatcaaa
    12901 atatggtgat tgatatctaa ttgataccat tcaagtaaag taaattccgg attatgccaa
    12961 cgccctaact cgtcatcgcg aaaaactcgc gctaattgaa aaataggccc gctgccagca
    13021 gctaacagcc gtttcatatg gtattccggg gaagtttgta aatagcatgt ttcacctctg
    13081 aaaatcgctt tgatattgct caggtaaaca tctgtaaccc catgccgcgc catcacaggg
    13141 gtttccactt ccagatattg gcgctcagta aaaaattgac ggattttacc tattagctcg
    13201 gctcgcgcac gtaataattg aattgaagca gagggcaacc aggagttatc agacatgatc
    13261 aataaaatat tcccaactcc aagcgggcag cctctgtcat gcggtcctga gaccagggcg
    13321 gctcccacac cagctcaaca gtgcaatcac tgactccttc aacctgattg actgcctgct
    13381 ccaccgtacc aggaaaggtt tgcgccacag ggcaccccgg ggtcgttagc gtcatctgaa
    13441 tatgaacatg gccttcgtca ttaacagcaa tgtcataaat caagcccaga tcataaatat
    13501 tgaccggaat ttcgggatca aaaactcccc tcaacgcagt aataacgcct tcctttattg
    13561 attcgttact ctgttttttc ttaaaaccaa acatgagctg cctctggtct attttccaat
    13621 atacagacaa actctttcac gagccctgcc actattgcca taacattatt ctgttttcac
    13681 tacttctgtt tctttgttta aagccgcttc caaagtatgc cacgccaaag tggcgcattt
    13741 tacacgcgca gggaatgctt taactccagc aagaactgtt aatttatcca tggaaaccaa
    13801 agaatcttct tcctcttgtg ttagcattcg atgaacacga tgaaatagct catgagcttc
    13861 tttaattgat ttacccttta acgcatcagt cattaacgat gcagaagctt gtgagatagc
    13921 acagccacat ccaacaaaac tcacatcacg aatcaaatca ccctgcaatt taaggtaaac
    13981 agtcagttta tccccgcata aagggttaaa ccctttcgcc tcggtagtcg catcctccat
    14041 cacatgatgg ttacgaggat tacgattgtg atcgatgatg atttcctggt aaagttcccg
    14101 caactccata ctcatgcgaa tacctcttta actcgttgta aggcttccat acaataatca
    14161 atttctttaa acgtattata aaaggacatg gaaatacgag aggtcgcagc aacatcgtag
    14221 aaatccatca aaggcatagt gcagtgatgt ccgctgcgta ttgctatacc ttcactatcc
    14281 agaatagttc caatgtcatg ggcatgaatt tttccgtgga caaaagagat aatgggtact
    14341 ttgttggcag ccgttccaat aatattgtac ccttttactg cttcaatagc cttggtagca
    14401 tagtttaaca attgtgtttc gtattcagct atcgcatcca aatccaatga ccaaatataa
    14461 tcaattgcag cagccaaacc aatagcccct gcaatattcg gggtgcctgc ttcaaattta
    14521 tgggggatag ccgcatattc tgttgcctca aaactcacgg agttgatcat ttcgcccccg
    14581 ccctgataag gagtcatgct atttaataat tcttcttttc cccataaaac acctattcca
    14641 gtggggccat acattttatg tccggaaaaa gcatagaaat cgcaatccag atcctgaacg
    14701 tccacaatca ggtgagccgt tgcctgtgcc ccatccagta acactttagc ccctacttca
    14761 tgtgccatct taatcattgt tttaacagga ttaatagttc ctaaagaatt ggaggcgtaa
    14821 ttaatcgcaa ccattttagt gttttcattt aactttcttt caaactcttc aagtattacc
    14881 tcgccattta aagaaatagg ggcaacttgc agcttgcaac ccattttctt acagaccatt
    14941 tgccagggaa ctatattgga atgatgttcc atatgcgtta tcaaaatttc ttcatccggt
    15001 aatatgcgag gagctacgag actctgggct actaagttaa tggcttctgt cgtaccgcgc
    15061 acaaaaatac attctctcgg tgatctggca ttaataaatc gttttacttt cgctcttgct
    15121 gcctcataca tttccgtagc cctgacactc aaggcatgaa ctccacgatg tacgttggaa
    15181 ttatcctttt cataaaattg agcaatagca tcaatcacag ccttgggttt ttgagtagta
    15241 gccgcgttat caaaatacac caaatcatac tcattgattt tctgatgcaa tacagggaaa
    15301 tctttcctga ttttgttgac atcaaaggat gtcattaatg aaggattagt actcatttgc
    15361 gtaccccaac tgttgagtta ataaattacc catccaatca gccaaatcac gatttggaat
    15421 aaggcgcaaa ttatcattgg caaatgcctg gatcaaataa tgagaagctt catcccggct
    15481 aataccacgt gttgccaggt aaaatattgc ctcttcatcc agttgcccaa ccgtagcgcc
    15541 atgtgaacat aacacgtcat cggcgaaaat ctctaattgc ggtttggtat caacttcagc
    15601 atttgcggag agaagtaaat ttttattttg ttgcttggca tcagtatgtt gcgcattctt
    15661 ggctacaata accttgccat taaataccgc tctggaatgt cctgttaaaa ttcctttgta
    15721 atcttgatcg cttcggcagt taggcactaa atgatgaact acagtatgat gatctatgtg
    15781 ttgcccatcc cccggcgcat aaattccatt cataaggcat tgagcctgtt cttcctgcat
    15841 gaacacgcta atatcactgc gtaccaactt cccacccaaa cttaaagagt ggctggaaaa
    15901 ttgactggcg gcagattgcc taattgataa atgacctaaa tggtaagctg atttactttc
    15961 tttttgaatt ttataatgat taagttttgc tcctgctcca agaaacactt cagtaatact
    16021 gttagtgaaa tagcaaatgt tttcttcgcc ttgatattct tcaatcagag tagcctgaga
    16081 ttctttttcc ataaggatca gatgccttaa atacatagcc tggttcgttt gatcctggat
    16141 atgctttaaa acaatgggtt cttccaaaca aattcccgca ggaatataaa taaagactcc
    16201 gcattggagc atggctgtat ttagataatg aaacccatgc tcctgctcta caatttttcc
    16261 aaggtaaggc ttaaccaaat cagcataatg tattaaagca agagataaag gcagaattaa
    16321 cacgccttca ggcagattct taatccattc ttgctcttgg gaaataaatc cattctgtat
    16381 taaaaacagg tttttgactg gtgaatcgta aaagacggct ttaagagcag gctttactgt
    16441 aggatcaggt agtacaaaat gttgctgtaa tagtgagtca acgagagtgt acttccattc
    16501 ctcatcatat cgggtgggaa aaccacggcg cttcaaatca gttaatgctt tagactgcaa
    16561 ctgcgcaagc cagggtattt gcgatacacc tgattgggct tgctgttgat aataatccaa
    16621 aatatcactc ataactgctc cacttcttca agccagctat aacctttttt ctccagttcc
    16681 agcgccaaag atttatcccc tgacgtaata atacgaccat ttgccaagac atgaataaaa
    16741 tcaggctcaa tataatccag taagcgctga taatgggtaa ctaaaataat agaacgctcg
    16801 ggagagcgca tcgcattaac tccttgagaa ataattcgta atgcgtcgat atccagaccc
    16861 gagtcggttt catcaagaat agctagcctg ggctccaaag cagccatttg caatatttca
    16921 ttacgtttct tttccccacc tgaaaatcct tcattaatac ttcggtataa aaagctttca
    16981 tccatatcca gaagctgaca tttttcccga ataaaactta aaaactctat ggcatcaagg
    17041 gtgtttcttc cttgtccttt gcgaaccgca ttcactgagg ctttaagaaa attgatattg
    17101 gttacaccag gaatttctac cggatattga aaagacataa aaataccggc ccgcgctctc
    17161 tcttccgggg acataggcaa caaatcttgt cccaaatagg atatatcacc acgagttatt
    17221 tcataagaag gatgcccagc caataccttg gagagagtac tttttcctga accattaggt
    17281 cccataatcg catgaacctc tcctggttta acctcaagat taataccttt taaaatagac
    17341 tgtccattga cagcaacatt gagttcttta atgtttaaca tattagccga ctgccccttc
    17401 taagcttata cctaataatt tagtggcttc caccgcaaat tccataggta attcttttaa
    17461 aacttgctta caaaatccat tcacgatcat agaaacggca tcttctgtat cgataccgcg
    17521 ttgctggcaa taaaacaatt gctcttcact aatttttgaa gtagtggcct catgctctac
    17581 ttgtgccgtt ggatttttga cttctatata aggaaaagta tgtgctgaac attgactacc
    17641 cattaacatg gagtcgcatt gggtataatt acgcgcgtta gtggctgttg gtgcgatgcg
    17701 taccaatcct cgataagcat tatgcgcaca cccggcactg atccctttcg aaataattgt
    17761 tgatcgagta tttttcccta aatgaatcat tttcgtacct gtgtcggctt gctgaaaatt
    17821 attggttaag gcaacagaat aaaactcgcc aacagaatca tcaccttgta aaatcacact
    17881 ggggtatttc cacgtaatgg ctgaacctgt ttcaatttgt gtccaggaaa ttttcgattt
    17941 cttaccccga caagctcccc gtttggtcac aaaattgtat atccctcctt taccttcttt
    18001 atcaccagga taccagtttt gtaccgttga atattttatt tgagccccat ctaacgcaat
    18061 caattcaacg actgcggcat gcaactgatt ttcatctctc attggggcag tacacccttc
    18121 caggtaggac acatagctgt caacatcagc aacaataagt gttctctcaa attgcccggt
    18181 agaagcagca ttgatcctga aataggtcga tagctccatg ggacatcgga cacctttggg
    18241 gacataaaca aatgatccat cactaaacac agcagagttc aaagcagcat aaaaattatc
    18301 cctgtaagga acaactgacc ccagatattt cttaaccaag tccggatatt gatgtaccgc
    18361 ttccgataat ggacaaaaaa ttactccttt ttcagctaat ttcgctttaa aagtagtcgc
    18421 aactgaaaca ctgtcaaaca ccgcatcaac cgcaacacct gccagcattt cctgttcgcg
    18481 taaagggata cctaatttct cataagtttt taacaactcc ggatcaactt catccagact
    18541 tttgggagcg tcttttttac ttttaggcgc ggaataatag gaaatggact gataatcaat
    18601 tggaagatag tgcacactcg accattcggg gtgaggcata gtgagccagt gccgaaatgc
    18661 tttcaaccgc cactcaagca aaaattcagg ttcgcctttt attgcagaca aacgacgaat
    18721 gacatcctca tccaatccgg gaggaaaagt ttcaacttct atgtcggtta taaaaccatg
    18781 ttgatattct ctatcgagca gagaatttag ttgctcactg cttttagcca cgatttactc
    18841 cacttgctaa ttgccgaact cgctcaacat cccttgttgg aagagttggt tttgccaatg
    18901 cctccaaact gacactatca agagcagttt cgatcgcatg gcttattaat cgccaatttc
    18961 cttgaatggt acatactccc tgtaaaaaac aatcattggg ttgcatactg cattctgtga
    19021 aacccctgtg ctcttccaaa gcaaatatga tttgagacac cgaaatttca gctgctggtc
    19081 tttgcagtct atatcctccg gatacacctc gtaccgaagt taacaaaccc gcgctagtca
    19141 ggcgttttag tattttacta acagtaggta ctgttaaatg cgtatgtaag gcaatatctc
    19201 gagcattgca taattcctgc gaatgcttgg ctagataaac cattacaact gttccataat
    19261 cggccaattt actgacgcgc agcataacac cccctactcg aataatctag taccaaataa
    19321 gtcttatttt cagatagagg agaagaatat cttattaaga acaaggatta gacaattgct
    19381 tactgatcca atatcacctg atcctctatg tgagtttttc tggcacaatc atccaaagca
    19441 aaattctatt accttattat aaatatagta ctaatttggt tctgtattcg ataaaagaga
    19501 gattttacct tattgataag gaaataagca aataaaatct tctgttctac cttggcgatt
    19561 tcatgcattt taatattttt tacaataggt tagtcatcaa gggaatgggc ttgccaatat
    19621 tccatctgtc tataatgtgc tctggattta atatagaata ctcttgataa acacaaccaa
    19681 acccataaga ataagctatt cgattatttg ctttcaggag ccaggatgac tcacgacact
    19741 gaactaagcg aactgatgca ggctgtagct aaaaaaagcc tgcagattat gaccgatttc
    19801 aaagaaaaac ccatcccgat ttcatcgctg gtcagtcaat acatcgatct cacggagcat
    19861 ttccaaaatt tgattgccgt cattctaaaa aatcctgaaa aagtatggca aatgcagttg
    19921 aattacctag aagatgcctt aagtctggct caagcacaat ttaattattg gcttgaagga
    19981 aaaccactgc ctattaacga tcagcgtttt aatggggaag attggataaa caatcctttt
    20041 tttaacttgc taagccaaca ttatcttttg gcaaatgagc atatgaattc attgctggaa
    20101 aatatggaat atggtgacga gaatctggca aaaagagtac gttttttcac ccgacaatat
    20161 cttgatgctt tatccccagc taattttatt cacacgaatc ctcagctcat ggcagaaaca
    20221 ttacaaagtc atggtaaaaa cctattacgt ggattacaca atttgctatc cgatgttgaa
    20281 gcaggatctt ccagattaat cattaaaatg actgatacgg aagcatttaa aatcggtgag
    20341 aatctggcta caacgcctgg taaagtcata tttcgcaatt ccatgatgga gttaatccaa
    20401 tattgcccca gaacaacaaa agtaaaatcc atacctttac ttatcgttcc tccctggatt
    20461 aataaatact acattttaga cttaagcccg cataattcat taatacgctg gttggtcgaa
    20521 caagggatta ctgtttttat tatctcctgg gtcaaccctg acgaaacgta tgccaataaa
    20581 agcttctatg attatttaaa tgaaggcccc agagaagcca ttgcagtaat tcaaaaacaa
    20641 ttgcgtgtta aacaagtcaa tacgctggga ttctgtatag gcgggacact tctggcttct
    20701 cttttagcct acaataaggc aactaaggat cattccattc gcagtgcaac ctttttagcg
    20761 gcaatgattg atttcagtga tcctggggac atcgctgttt ttattgatga gcagcagatt
    20821 aataaacttg aagaagaaat gaaatccaaa ggttatctgg ctggcaaatt tatggcgtcc
    20881 agctttaatt ccttaagggc aaatgaccta atatggtcat ttttcatcaa aaattacctg
    20941 cgcggtaaaa gccctgttcc ttttgacata ctctattgga atgctgattc aactaatatg
    21001 cctgcaacca tgcattctca atatttacgt tggatgtatt tgcataataa cttgataaaa
    21061 cctggaaaaa ttcgccttaa tcatatccct attgacgtaa caaatatcga catacccact
    21121 ttctttctgt ctactcaaaa agatcatatc gctccctgga aaacaacata taaaggattt
    21181 gaattaatga aggggccaaa acgttttgtt ttaggtggat ctggacatat tgcaggaatt
    21241 attaatccgc caacccagca aaaatatggt tatagaacca ataacagcat ggatttatct
    21301 gctgagcaat ggtttgaaaa atccaaggaa cactccggtt catggtggcc agaatggtta
    21361 aattggctta aattgcattc cggaagactc atcaattccc ctgatatcaa tcacttgcca
    21421 ttcgcaccga taatggatgc tccagggagt tatgtactaa aaaaataata ttttgaactc
    21481 aataaccata cattaaaaca taaaaggagt tgtattgtga gaaaaaaaaa caggatactt
    21541 ttggcaagtt tattcttgct aggcagcctg aatgcctttg ctgctcaaga tctgacggtt
    21601 gttaatacgt ccgatgatct gggatatgtg acaataggat cctattcctg tgaattgact
    21661 tccagttgtg ctattccccc tgataattcg attcatatta actctgatat cttagaagca
    21721 gcttgtgcga atacttccgc tgattgtgaa attgtttttg gggttgacag tataaaagca
    21781 agaatcgcga ccggaagctt tagtttgaat gaggggatac aatacatcgc ccaatctcgt
    21841 tggctgaatt attctgctaa gcgtttatcc aaaaccgtgg ctgaaatcta taaaagataa
    21901 ttcgatttat tccctgctga tgtcctggtt gaagtacaca gcaatagcca ggacaaggtg
    21961 caatgacatg gattgattaa gataaatcat aaacacaggg atctgatttt ctccaccagc
    22021 tctctgttct tgccaaaatc agaatacccc atggtggcac ttgaaataac attcccatcc
    22081 gcatggatgc atagccaatc aacaaaatgg aaaaaggggc tttgtctata ccccaccaga
    22141 aaggatgcct cctgtttctg gattttcaat tccgggatct tgcgttcaat gcatgctacc
    22201 aattgaggca tactctcaac cttcaaagga gcaatatagt gagaatccga ttcattaacc
    22261 aggctactta cccagtttgg cttatcatct ggcaattgcc cctcaactaa ccctttcggc
    22321 atttctcgaa aattgatttg aggaaataaa taaagaatga caacaagggc taaaataagg
    22381 gctaatacta tccatattaa tttcatttat actgtttcca tggtttcttt ctgttctaaa
    22441 aattgctttt caagcgttat ataatcaatt tttcctgaag gcaggacagg aatttggcat
    22501 gccggataaa tcatatgagg aaccagcaat tctgattgcc ctctctcctt aacctttttt
    22561 ataaatgata ctttatctgc atactctgct tcagtaaata agaaaatttg ctcccccttt
    22621 cttggagatt tttgcacaac agcggcatgt aataattcag gccaaattga ggctgcaatt
    22681 ccttccaccg ctgtaagaga taccatttca ccagcaatct tggcaaaacg tttggcgcga
    22741 cctgctatag taatgaatcc atccgcattc acggtgacga tatctcccgt atcatgccaa
    22801 ccatcttcag gttctataat tactccaggt ttatcctcat caagataacc taacatgata
    22861 ttgggtccgc ataattttaa acgcccgcct tgtacaattc cctctactgg ttctactttg
    22921 aaatccataa aaggtaaaat cattccgaca ctacccggta ctgatgccaa aggacaatta
    22981 agcgatatga ctggcgatgc ctcagtagca ccatagcctt cataaattct tgccccaaac
    23041 gcttcgctcc agtatctaat ggtttccggt ttcactttct cggcgccagc gaagatatag
    23101 cggacgctgt tgaaatcatg tttgcccgcg gcccttgcat aacctgttaa aaaagtatct
    23161 gtgccaaaca aaatagtagc ccttgtttga taaatcaatc caggaattac cttataatga
    23221 agaggggaag ggtagaaaaa acaattcaaa ccattaacca aaggaagcac acttcctgct
    23281 gtaaggccaa aacaatggaa tataggtaag gaattaaata aaacatctcg tggagtaaag
    23341 tcgactctgg aagtcatttg ccaacaatta gccaatatat tggcatgact aagagcaacc
    23401 cctttgggtt ttccctcaga accagaggta aacaggatta tgcctgttgt atcagaggat
    23461 attttgtcac caagaaaacg gtagcttaga gtaggaaaga agcctttgaa tatcccggaa
    23521 agcttgttac ctaaatgaat ggttgattta aaatcttcta aataatgaat tcttataccc
    23581 gcctgttgga actcttctac caagggttct aatttggcgg tttctatgaa ttgtcttgcg
    23641 gtataaattg atttaattct tgctgtatta caagctgaat ataagttata aaaacccatg
    23701 ctaaaattta acatggcagg tattcgtcta taagcttgta aagcaaaaaa agtgaccatg
    23761 ccagctatag tagtcggcat cattactcca acagtttcgc ctacagccgt ttgcttttta
    23821 atttggcgcc ccagaataaa acaacgagcc aagaa
//
LOCUS       DASLOE010000032        23237 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.032, whole genome shotgun sequence.
ACCESSION   DASLOE010000032 DASLOE010000000
VERSION     DASLOE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.032"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            61..1215
                     /gene="bamB"
                     /locus_tag="U6309_001025"
     CDS             61..1215
                     /gene="bamB"
                     /locus_tag="U6309_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEN5504478.1"
                     /translation="MKIRILVLILCALTQGCTYVDDYMLGKDNTPQPKELKEIQPKVK
                     MAQSWTTPVGKAHKTNEYLNIKPAIRGDIIYTADASGLVQAVNRKDGQIKWSTALKNN
                     IVSGPTVAAGYVAVGTNTSTLVLLNQSDGKEIWQNKVSAEVLAPPAISHQKVIAKTID
                     GKVYAIDAVNGKQLWVADHGAPSLVLKASSSPIIVDDLVLVGFSDGKLDALELQTGRL
                     IWQRSIAYGTGASDVERLVDIDSDPIISNNVAYLATYQGYVGALSLSNGQFIWRKPAS
                     VYKNMLLSHNNLYFTDSNDVLWSLNSSTGQVNWKQTSLKARGLTAPALVGGNLAVGDK
                     TGYLHILSTQTGELLGRSQLSGGVTVSPSVSGKNMYVLTNNGMLNQLSVS"
     gene            1220..2608
                     /gene="der"
                     /locus_tag="U6309_001026"
     CDS             1220..2608
                     /gene="der"
                     /locus_tag="U6309_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEN5504479.1"
                     /translation="MIPVIALIGRPNVGKSTLFNRITKTQDALVADFPGLTRDRQYGH
                     AQHENKSFIIVDTGGIGVDDIEVDTLMSRQSQVALNEANVILFLVDGRSGLTGIDQQI
                     AQALRKLNKKVHLVVNKTDGMNEDIACADFQSLGITDIHAISASHGGGISSLLEEILE
                     PFTTETHEATDDKAIKIAFAGRPNVGKSTLINRILGEERVVVYDMPGTTRDSISIPFT
                     REDKQYVLIDTAGVRRKSRIDEKIEKFSVIKTLQAIKEAHVCLLLLDANEGITDQDMN
                     LLGFIIESGKALVIAVNKWDGLEEDHKEKIKSELSRRLHFANFAKIRFISALHGSGVG
                     GLFKDINEAYHSAIQSFSTPKLTRLLQDISAKHTPPCINGRRIKLRYAHLGGHNPPVI
                     VIHGNQLDALPESYKRYLNNEFIKHLGLVGTPLKIEFKGGQNPFANKKNKLSQRQVNK
                     KKRLMRWAKSKK"
     gene            complement(2686..3108)
                     /locus_tag="U6309_001027"
     CDS             complement(2686..3108)
                     /locus_tag="U6309_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN5504480.1"
                     /translation="MYTNILFATDLLNEHSHLTEKAANIAKQFNAKLYLLHVIELPTS
                     ILIAQGLGFTELANPSKEDAQTVLSLIGENLQIPKEQQFVEIGSVKEHILNKAKDLNC
                     QLIIIGSHSATGLPSILGSTAHAVVNHSVCDVLTLKAD"
     gene            complement(3163..3588)
                     /gene="folK"
                     /locus_tag="U6309_001028"
     CDS             complement(3163..3588)
                     /gene="folK"
                     /locus_tag="U6309_001028"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEN5504481.1"
                     /translation="MNVCYLGLGSNQKNPERQIRQAIKEIKQIPSTCLIKVSRLYWNK
                     AWGFENQQEFCNVVIEIRTTLLPHKLLKWCQKIENRHKRVRRKFWGPRTLDIDIILYG
                     YRLIRTKNLIVPHPCFHLRDFVLVPLRELNSNLKIPNYT"
     gene            complement(3585..4856)
                     /gene="pcnB"
                     /locus_tag="U6309_001029"
     CDS             complement(3585..4856)
                     /gene="pcnB"
                     /locus_tag="U6309_001029"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEN5504482.1"
                     /translation="MAYIIPRNKHNISKADISNNALKVLNRLISHGFQAYIVGGSVRD
                     LLLHKAPKDFDIATNATPNEVKNLFKNARIIGRRFKLVHILFHREIIEVATFRGHNSS
                     DASHQFNERGMLVRDNVYGSLDEDAWRRDFTINSLYYNISDSSIIDFTGGVEDVKQKL
                     IRIIGDPVTRYQEDPVRMLRAVRFSAKLQFNLSPETKAPFPKISHLITHVSSSRLFDE
                     ITKLYQCGEAESVQKLLVEYGLFNYLFPQTAALFDSHYPVNPLLSIALENTDTRIRDG
                     KPVTPAFLIAVLLWFPMIECSRKLQESGLPPLPSIEKAMSIVLSEQNAIISIPKRYSQ
                     VIREIWLLQYRFEKRFGGRAFNLLLHPRFRAAYDFMALRALSGDESMELAQWWATFQE
                     VDDVEKNNMVTQLAATTPGRPKKRRKRKIAK"
     gene            5163..5894
                     /locus_tag="U6309_001030"
     CDS             5163..5894
                     /locus_tag="U6309_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEN5504483.1"
                     /translation="MDFIDIGLSLTALIILEVVLGIDNLVILSILTEKLPREKRKKAR
                     RWGLTFAWMTRLLLLGSAVFLVKLVKPILTIGSLVFSARDLFLILGGAFLIWKSTDEI
                     HQDVMNEPLMEPVSKNVSSSATFRGVVIQIALLDIIFSLDSVLTAVGLTSHFMVMAVA
                     ITIAIIIMIWASEPVSKFISEHPTIKMLALSYLILIGTVLVADGFEFHVPRGYLYFAM
                     GFSLAVESLNLIKNERVKRKKLKRE"
     gene            5897..6319
                     /gene="hemJ"
                     /locus_tag="U6309_001031"
     CDS             5897..6319
                     /gene="hemJ"
                     /locus_tag="U6309_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HEN5504484.1"
                     /translation="MLFVKAFHIIALVAWFAGLFYLPRLYVYHAESLDKISVDRFKIM
                     ERRLYYGITWPAALATTFLGLWLFTYNLSYYLKAGWMHMKLALVLLLWVYHLTCGHFL
                     KSFALGKNTRSSRFYRFFNEMPTLLLVGIVLLVVVKPF"
     gene            complement(6316..6492)
                     /locus_tag="U6309_001032"
     CDS             complement(6316..6492)
                     /locus_tag="U6309_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEN5504485.1"
                     /translation="MQEYKRYICVICGFIYDEAEGWPEDGIEPGTRWEDVPENWFCPD
                     CGAGKEDFEMVEMD"
     gene            6915..8204
                     /gene="hemL"
                     /locus_tag="U6309_001033"
     CDS             6915..8204
                     /gene="hemL"
                     /locus_tag="U6309_001033"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEN5504486.1"
                     /translation="MSRSSDLFHKAQTIIPGGVNSPVRAFKGVGGEPVFFKSGKGAYL
                     TDVDDKQYIDYVGSWGPLILGHCHPKVIEAVDNVLHSGMSFGAPTELEIQLAEKIASL
                     MPSIEKIRMVNSGTEATMTAIRLARGFTNKNKFIKFNGCYHGHSDSLLVKAGSGLLTL
                     GIPSTPGIPQSITEHTLTADFNNLEQVAQLFEKYPNDIATVILEPVPGNMGFILPKIE
                     FLKGLRELCDQYNALLIFDEVMTGFRVGLHGAQGLFGIKPDITTLGKIIGGGMPVGAL
                     GGKREIMSFLAPEGPVYQAGTLSGNPLAMAAGLATLKEIEKINFFEDLSNTTNKLTEA
                     LADAAENANIPFFTASLGGMFGFCFTDKNSVENYLDVASSDEVLFRKFFHAMLAQGVY
                     FAPSMYEAGFVSSMHGDLEIQKTYDAAELVLNQLKSA"
     gene            8606..10132
                     /locus_tag="U6309_001034"
     CDS             8606..10132
                     /locus_tag="U6309_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5504487.1"
                     /translation="MLILSKRRGLWSSMPQGTFALFFIQVVSTLSFSVLYSTLVLYMK
                     GKLGLPIATANSIMGVFIAYNFALHLLGGFWGGRLLSNRSLFCVGMIAQIIGCVLLSI
                     GNTTFLYYGLAAFLTGSGLNVTCVNCMLTQQFAPEDSRRETAFLWNYAGMNIGFFIGF
                     SLSGAFQISQNYQRLFLLSSLGNLVALLICLYFWQQLHDKNTTYSRKDKPAQRKSTAL
                     GIGLIFVLPFILRQLLQYADWANKLVIVTGIVMLILILILAQQQSTKEARDKIYGFAV
                     LMLVGTVFWMLYQIGPMGLTVFIDHNVQRQYGNWIIPPQWFQNINTVVIVFGGPLLGF
                     ILNRMRQNGSQINIPTQFAFALFFIGVAFAILPIGINYANQHGMVNPGWVVISFILQS
                     IGELLISPIGYAMIGALAPTSLQGVMMGMWMLNTGVGATLSSYSSNLMIIGQESTSPL
                     LTNEGYSHVFLMLGLFAIGSSVILFILVPKLRKLIKEKNLNSFKQSGIEVTERVALCE
                     "
     gene            10125..12125
                     /gene="mnmC"
                     /locus_tag="U6309_001035"
     CDS             10125..12125
                     /gene="mnmC"
                     /locus_tag="U6309_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEN5504488.1"
                     /translation="MSSPFVPIITADINWRDDLPYSLQFDDIYYSAEGGINQSLYVFV
                     EGNNLINRWQQLPTNESNVFTIAETGFGTGMNFLLTWKLWEKFAPQNARLHYISCDKH
                     PLKKNDLIKCLQKWPELSVQTEKLIEHYPVLTPGYHHLAFSNNQITLTLMLGDVLECY
                     EQLLFCGDINLEHQLRESYVNAWYLDGFSPSKNQSMWSDNLFTVIAMLSKESTTVATY
                     SASSIVKTALTNAGFVIEKRKGFGPKRHMICAHYEKAYSSSKKNRHTPWHINYPVTKD
                     ERTALIVGGGLAGCFIANSLAKRGWEVTILEEKEKVGCGGSANQQAVLFPKLSTYKSP
                     FTQFMLYSFLYANDVYKELLKHYDLGELKGSLLLAHNEREKANQQSLIHWLELYPELG
                     QLVDEKQSSELSGISLPCGGLFIPSSGWINSPELCDILIDNKRISLITGNRVQSINYN
                     QKNWVVNDIEASVLILANGQQVNYFYETNHLPVKAIRGQMTTIQSTQESIKLKIPLCA
                     EGHVLPALNNSHRVGASYDIGTSEPELNALDDQLNLDRLKRIAPDIMWSQNVLDHWAG
                     IRAASPDYLPIVGPLPNALEFKEIYAELKSNSKRWIAEAAPCYPNLYVCAAFGSRGLT
                     TIPLATEWLAGLINKEISILPRKLIQAISPARFLRKKIIQGP"
     gene            12211..12996
                     /locus_tag="U6309_001036"
     CDS             12211..12996
                     /locus_tag="U6309_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEN5504489.1"
                     /translation="MQNIEFYQIDAFTDKVFHGNPAAVCILDDWLNDELLKAIAAQNN
                     LAETAFIIKDDQDFHIRWFTPNGEINLSGHATLAAGFALLESNKGPSDSIIFHHSSGQ
                     ISVEKRKEKFVLNFPRIGYKKTPSPDPIVSLLDQPAIEVFETDLDYLVLLEHESQIRN
                     LQIDLFALAKLPKRGIIVTSTTTEADFYSRCFYPKHSIAEDPVTGSAHCILAPFWAEK
                     LNKNSLYAIQGLHRKGELYCEVQRNRVLISGHCRWYLRGHLVI"
     gene            13212..14330
                     /gene="prpC"
                     /locus_tag="U6309_001037"
     CDS             13212..14330
                     /gene="prpC"
                     /locus_tag="U6309_001037"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEN5504490.1"
                     /translation="MSSKSGGLAGVVAGQSAIATVGLAGKGLNYRGYSINDLAEYASF
                     EEVAYLLHYGELPTQKELDVYIKKLVNLRHIPDVLKTVLKLIPKNTHPMDVLRTACSF
                     LGTIEPEENFKQQYEIADRLLALFPGIMCYWYTYHFKNKEISGLSEEDTTGGHFLALL
                     HGRKPSKIEADMMNVSLILYAEHEFNASTFAARVTAATLADFYSAITSAIGTLRGPLH
                     GGANEAAMELIERFKTPDEAAIGIKQMLASKELIMGFGHRVYTTCDPRSDIIKKWSFR
                     LGEEKDNLLLYHISERIEEIMWQEKKLFPNLDFYSASAYHYCDIPTHLFTPIFVMSRI
                     TGWSAHVFEQRANNKLIRPTSEYIGPDSRPFPAIETRG"
     gene            14332..15780
                     /locus_tag="U6309_001038"
     CDS             14332..15780
                     /locus_tag="U6309_001038"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HEN5504491.1"
                     /translation="MHNYVEDNVKPDYDQVITDIADFVLNKEIDSVQAYETARLCLMD
                     TLGCGILALNFQECTKLMGPIVPGATLPGGARVPGTNYELDPVQAAFNIGTMIRWLDF
                     NDTWLAAEWGHPSDNLGAILAVADYLCREKCNHGRPPILMHDVLTAMIKAHEIQGCLA
                     LENSFNRVGLDHVILVKVASAAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNA
                     GSRKSWAAGDATSRAVRLALIAKAGEMGYPSALTAQTWGFYDVLFGKKQFKFQRPYGS
                     YVMENVLFKLSYPAEFHAQTAVECAVKLHPIVKNRLEDISRIELITHESAIRIISKQG
                     ALYNPADRDHCLQYMVAVGLLFGDLKAEHYEDDVAADPRIDALRAKMHVTENEQFSRD
                     YHDPEKRSIANSMRIIFKDGSESELITIEYPIGHKRRREEGIPVLMSKFKRNLSTRFS
                     TDMVEKIIHAMSDTNTLSSMKVSDFMAMWMVN"
     gene            complement(15900..16715)
                     /locus_tag="U6309_001039"
     CDS             complement(15900..16715)
                     /locus_tag="U6309_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEN5504492.1"
                     /translation="MKRYVFMLSDGTGITAETLGNSLITQFENIQFEKITIPYIDSTH
                     RAESVVLRINQCFSEQGTKPLVFMTLVDPEIRQAIKKAHACVFDLFSIFIGPLENELE
                     EKSSYTVGRTHGVANVKSYSHRIEAIDFALSHDDGIKTRGYDKADIILIGVSRCGKTP
                     SCLYMALQYGILAANYPFTEEDLVGFRLPEVLRPYKQKLFGLTIDAQRLQQIRSERRP
                     NSKYASAEQCRLEVTEVEAMYQRENIPYINSTKYSIEEISTKVLAIAGLQRKI"
     gene            16849..17304
                     /locus_tag="U6309_001040"
     CDS             16849..17304
                     /locus_tag="U6309_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504493.1"
                     /translation="MQHNIYENSKLHILGIICLVTSLGLFFFSLYIVPFLIWQLDYDI
                     PDFISNMIAYGEDEYYFSPITSKTLVWLIFFTPSIIMGYLSYYISNYIDKSYLEPQEK
                     PLESMQEKSPSKPAGRGRDSTILGLKIVLLMVLIVAIIFLLQILFDLTA"
     gene            17320..17913
                     /locus_tag="U6309_001041"
     CDS             17320..17913
                     /locus_tag="U6309_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504494.1"
                     /translation="MFIFKRWKIRKITKRIKAMQANRVSNQPGDEVLKKEILYYFELA
                     TIFKKLKNHKKYPYAEIMMIECYRAAANLDDSAANFQLGQIFLDEAKYRQKLDNEGIF
                     NSQANLKRAQQLFDEAHAHLIAAEKLGHVGAKRLRGLCIINGWGVESDKNAGFELVVD
                     SIEQEGSWDKIPQIFASMGLNKPEFFSAIMQRRKGTS"
     gene            18021..19226
                     /locus_tag="U6309_001042"
     CDS             18021..19226
                     /locus_tag="U6309_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEN5504495.1"
                     /translation="MNQNDASKKDNGNSNQLDTELIGSYQSITIGFSKDELASFSKIG
                     QHKHYAAGSIVYNENDLADSFFIIIDGVAEIFKKTIDEFGEHNIHILATLSKDDVVGE
                     MALIENSRRSASVKAKTDLSVIEYPLEVVKAKPKLNLMLIKNMAQILSRRLRFTNEVT
                     VKNMQQALKEASGRNTLGVFIIALLWIICLYTLSLHFLVTVIHQVTVTTVLSICLISI
                     FAVAILIAMHFTGLPYSRFGITAKDWGKTILEALAFTIPIMLFVLIIKVFFIIGIDHL
                     DNTPIFSGTKRFLVNNEVDWEYYFAMIFLYSLFAPIQELMARSALQSTFFHFLPGEKL
                     FRQWNGIFLSNLIFATMHTHLGLTFASLTFIAGLFWGWLFHRQKSLLGVSVSHVILGV
                     WSLFIVGLR"
     gene            complement(19240..20103)
                     /locus_tag="U6309_001043"
     CDS             complement(19240..20103)
                     /locus_tag="U6309_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEN5504496.1"
                     /translation="MINALIINYPWFMYLVVGLFSLAVGSLLNVIIYRLPIILQEEWK
                     EQCCELFQLEQRKEKIKLNLFLPRSFCPHCKAMVKAWQNIPLLSYILLRGRCYQCDSP
                     ISIRYPFVETLTTVLSLYASWHFGFTIQLLFALLAIWILISLVFIDLDHQLLPDSLTL
                     GLLWIGLIANTQNIFVSLGVAVLSCAGAYLALWLFINLFYLMTGKVGMGHGDFKLFAA
                     FGAWLGWMYLPLILLISSITGAIIGLIYLKINGKSRDTAIPFGPFLCISGLIAMFWGD
                     SIINWYIGYWM"
     gene            complement(20149..21369)
                     /locus_tag="U6309_001044"
     CDS             complement(20149..21369)
                     /locus_tag="U6309_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEN5504497.1"
                     /translation="MDKNSPPLLTFHYQGINKAGQKMEGDIQARSLAIAKADLRKQGI
                     VTNKVVKKRKPLFDRKNKKITQADITVFSRQLATMIESGIPLVQAFDIVAKGQSNKRL
                     KDLIETIKHDIETGLTLAESLIKHPLYFNELFCNLVDAGEKSGSLDIMLDKVATYKEK
                     IEIIKKKIKKALTYPIAVMVVALLVTAGLLIYVVPQFESLFKGFGADLPAMTRAVITM
                     SEFMQAYWYIIFGALGGIVYSFFYAKNHSLEFAQIIDRVMLKFPVIGPILEKAAIARF
                     ARTLSITFAAGLPLVEALKSVAGATGNIIYAKATDKIREEVATGQQMFIAMENTHLFP
                     NMVIQMVAIGEESGALEKMLSKVADFYEEEVNNAVDALSSLLEPIIMSILGILVGGLV
                     VAMYLPIFKLGSAV"
     gene            complement(21375..23099)
                     /gene="pilB"
                     /locus_tag="U6309_001045"
     CDS             complement(21375..23099)
                     /gene="pilB"
                     /locus_tag="U6309_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEN5504498.1"
                     /translation="MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLLQ
                     YIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTIPANLVNEKLIKRHAMVPLFSRG
                     TNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
                     DSGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYERE
                     YRIRYRQDGILHEVATPPASLSSRITARIKVMSNLDISERRIPQDGGFKMKISKSRAI
                     DFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLKAIQRPQGMILVTGP
                     TGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFL
                     RQDPDIIMVGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIA
                     SSVTLIIAQRLARKLCNQCKAVRDDFTNQGLIELGFKETDLVNLKLYKAVGCEQCTSG
                     YRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKEGITTIE
                     EVNRVTVD"
BASE COUNT         7359 a         4184 c         4339 g         7355 t
ORIGIN      
        1 atgattactg atgataaaaa ggtaaaagca gcttaatttt attataaagg tttgtttttt
       61 atgaagatta gaatattagt tttaatacta tgtgccttaa ctcaaggatg cacatatgtg
      121 gatgattata tgctcggtaa ggataatacg cctcaaccca aagaactaaa agaaattcag
      181 cccaaggtta aaatggcaca aagctggaca actccagttg gaaaggctca taaaacaaat
      241 gagtatttaa atatcaaacc agctattcgt ggcgatataa tttatactgc agatgcaagt
      301 ggattggttc aggcagttaa caggaaagat ggtcagataa agtggtctac cgcattaaaa
      361 aataatatag ttagtggacc aactgtagct gctggttatg ttgctgtagg aactaatacc
      421 tccacattgg tactacttaa tcaatcggac ggaaaagaga tatggcagaa taaagtatct
      481 gctgaagtat tagctcctcc tgctatctca catcagaagg tcattgcaaa aaccattgat
      541 ggtaaagtgt atgcgattga tgcggtaaac ggcaagcaac tgtgggttgc agatcatggc
      601 gcacccagcc tggtgttaaa agccagttcg tctcctatca ttgtcgacga tctggttctt
      661 gtcggatttt ccgatggcaa attagatgct ttggaattac agaccggacg attgatctgg
      721 caaagaagta ttgcttatgg aacaggagct agtgatgtcg aacgtttagt tgatattgat
      781 tctgacccta taataagcaa taacgtcgcc tatttggcaa cttatcaggg atatgttgga
      841 gcactatcac tgtctaatgg ccaattcata tggagaaagc cagcatctgt ttataaaaat
      901 atgcttttaa gtcataataa tctgtatttt acggacagca atgatgtctt gtggtcttta
      961 aatagtagca caggacaggt caactggaag caaacctccc taaaggcaag agggcttact
     1021 gcacctgcat tagttggagg aaatttagct gttggtgata aaacaggata tttacatatc
     1081 ctatcaacac aaacaggtga gttacttggg cgttctcagc tttctggtgg agtaacagta
     1141 tcaccgagtg tttctggtaa aaatatgtac gtattaacca ataatggaat gctcaatcaa
     1201 ctttcagtga gctaataaaa tgatccctgt catcgcttta attgggcgtc ccaatgttgg
     1261 aaagtctact ttgtttaata gaattactaa aacgcaggat gctcttgtcg cagattttcc
     1321 tggcttgaca agggatagac aatatggaca cgctcaacat gaaaacaagt cctttattat
     1381 tgttgatacc ggaggcatag gagttgatga cattgaagta gatactttga tgtcaagaca
     1441 gtctcaagtt gctttaaacg aagcaaatgt gattcttttc ctggtcgatg gccgttcagg
     1501 actaacaggt attgatcagc aaattgcaca agcattaaga aaacttaata aaaaagtaca
     1561 tcttgttgtc aataaaacag atggaatgaa tgaagatatt gcttgtgctg attttcaatc
     1621 attaggcatt actgatatcc atgctatatc tgcatctcat ggtggaggca tcagttcact
     1681 actcgaagaa attctcgagc catttacaac agaaacgcat gaagcaactg atgataaagc
     1741 gatcaaaatt gcttttgcgg ggcgccctaa tgttggaaaa tcaactttga taaacaggat
     1801 attaggggaa gaaagagtag ttgtttatga catgcccgga acaactcgag atagtatttc
     1861 aatacccttt acaagagaag ataagcaata tgtgcttatt gatactgctg gtgtacggcg
     1921 taaatctcga attgatgaaa aaatagaaaa gttttctgtc atcaaaactc tgcaagctat
     1981 aaaagaagct catgtctgtt tgttacttct tgatgcaaat gaaggcatta ccgatcaaga
     2041 tatgaatttg ctcggtttta ttattgaatc tggtaaagca ctagttattg ctgtgaataa
     2101 atgggacggc ctggaagaag atcataaaga aaaaataaag tcagaactgt caagaaggtt
     2161 gcattttgca aattttgcaa aaatcagatt tatttcagca ttgcatggga gcggggtagg
     2221 tggattattt aaagatatta atgaagccta tcattccgca atacaatctt tttctactcc
     2281 aaaactaacc agattattac aagatatcag cgcaaagcat acaccaccat gcatcaatgg
     2341 ccgacgaata aaattacgct atgctcacct tggcggacac aatcctccag tcattgttat
     2401 tcatggaaat caactcgatg ccttgccaga aagttataaa cgttatttaa acaatgagtt
     2461 tattaagcat ttaggattag taggtactcc tttaaaaatc gagtttaaag gaggtcaaaa
     2521 tccatttgcc aataaaaaaa ataaattatc acaaaggcaa gtgaacaaga agaaacgatt
     2581 aatgcgatgg gcaaaaagca agaaatagca ctccggttca cctcagccag gtttaaattg
     2641 ttctaatgaa aaaaaagata tcaattgggt actaatctct tcttatcaat ctgctttgag
     2701 tgttaataca tcacaaaccg aatgatttac tacagcatgc gctgtacttc ctagaataga
     2761 cggtaatcct gtagcagagt gtgagcctat tataattaat tggcaattca gatcttttgc
     2821 tttgtttaat atatgttctt ttacggaccc gatttctaca aattgttgtt ccttcggtat
     2881 ttgtaaattt tctccaatta atgataatac agtttgggca tcctcttttg atgggttagc
     2941 tagctccgta aaacccaatc cttgagctat taatatactt gttggcaact caatcacatg
     3001 taatagatac aattttgcat taaactgttt agcaatatta gcagcctttt ctgtaaggtg
     3061 gctatgctca tttaacaaat ctgtggcaaa taaaatattt gtatacatgt taacctcatt
     3121 ttaaacagaa ttattaagtt atagtgtagt agattatgat atctatgtgt aatttggtat
     3181 ttttaaatta gagtttaatt ctcttagagg aacaagaaca aaatctcgta aatggaaaca
     3241 aggatgtgga acgattaagt tttttgtgcg aattaatcta taaccatata aaatgatatc
     3301 aatatctaat gttcttggcc cccaaaactt tctcctgact cgcttgtgcc tgttttctat
     3361 tttctggcac cacttaagga gcttatgggg taacagagta gttcgtatct ctataacaac
     3421 attacaaaat tcctgttgat tttcaaagcc ccaggcctta ttccaatata agcgagacac
     3481 cttgataagg catgttgatg gaatctgttt gatttcttta atggcttgtc ttatttgacg
     3541 ctcaggattt ttttgattcg atcccaagcc cagatagcaa acattcattt tgctattttc
     3601 cgtttacgac gttttttagg ccgcccaggc gttgttgcag ccaattgggt taccatgtta
     3661 tttttttcaa catcatcgac ttcttgaaac gtggcccacc actgcgccag ctccattgac
     3721 tcatcaccag acaatgccct aagagccata aaatcatacg ctgctctgaa tcgaggatga
     3781 agaagtaaat taaatgctct gccaccaaat cttttttcga agcgatattg caaaagccaa
     3841 atttccctta tgacctggct atacctttta ggtatcgaaa taattgcatt ctgttcacta
     3901 agtactatag acatggcttt ttcaatcgag ggtaaaggcg gaagcccaga ttcttgcagt
     3961 tttctggaac actctatcat tggaaaccat aacaaaacag caataagaaa tgccggagta
     4021 acaggctttc catcacgtat tcttgtatca gtattttcaa gtgctatgct taaaagcgga
     4081 ttaaccgggt aatgactatc aaatagagca gctgtttgag gaaatagata attaaataaa
     4141 ccatactcga ccaataattt ttgaaccgat tcggcttctc cgcattgata aagtttcgtt
     4201 atttcatcaa atagtcgcga actggaaaca tgggttataa gatggcttat tttggggaag
     4261 ggagctttgg tttccggaga taaattaaat tgcagcttgg cactaaacct gacagcacgt
     4321 aacatacgga caggatcctc ttgataacgc gtaacggggt cgccaattat tcgaattaac
     4381 ttttgcttaa cgtcttctac accaccagta aaatcaatga ttgaagaatc agaaatatta
     4441 taataaagag agtttattgt gaaatcacgt ctccatgcgt cttcgtccag agagccataa
     4501 acattatctc ttacaagcat gccgcgttca ttaaattggt ggcttgcatc gcttgaatta
     4561 tggccacgaa aagttgctac ctcaataatt tcacgatgaa ataaaatatg caccagttta
     4621 aatcgacgac caatgatacg agcatttttg aaaagatttt ttacctcatt gggagtagca
     4681 tttgtcgcga tatcaaaatc tttaggcgct ttatggagca gtaaatctct gacactgcct
     4741 ccaacgatat aggcctgaaa accatggctg attaaacggt taagaacttt aagcgcatta
     4801 ttacttatat ctgctttcga tatgttatgt ttatttctcg ggatgatata agccaagtca
     4861 attgcatgtc catgcgacct gtttcttcgt aacagttttt taataaactt gatgattgtt
     4921 gtttctctct taattgatta aacagtaatt cggttgtgtt ttcaattatc ctgcaagttt
     4981 aagatttact ttacagaaat attcaattct ataaagctta acacgtaaac ttttcagatg
     5041 actatttaat ataaattgtt aacaccaatt aattataacg aagcaaatga tcaaagtgaa
     5101 tcatttacct ttacaaatat tttagagaaa ataatatatc ttgcatttta ttaggataat
     5161 aaatggattt tattgatata ggccttagtt taactgcttt gattattctt gaagttgttc
     5221 taggaataga taatctggtt attctatcga ttcttacaga aaaattacca cgtgaaaaaa
     5281 gaaaaaaggc gagaagatgg ggcttaacat ttgcctggat gacacgactt cttttactag
     5341 gctcagcagt ttttttagtt aagttagtaa aaccaattct gacaataggc agtctggtat
     5401 tttctgctcg tgatctgttc ctcatattag gaggtgcttt tttaatctgg aagtcaactg
     5461 atgaaattca tcaggacgtt atgaatgaac cattaatgga acctgtttca aaaaatgttt
     5521 cttcatctgc cactttcagg ggagttgtta ttcaaatcgc attgcttgat attatatttt
     5581 cactagatag cgttctcaca gccgtagggc taacatctca ttttatggtc atggcagtgg
     5641 caattaccat cgcaattatc attatgattt gggcgagtga gcctgtaagt aaatttatta
     5701 gcgaacatcc taccattaaa atgctggcgc ttagttattt gattttaata ggaactgttt
     5761 tggtagctga tggttttgaa ttccatgtgc ccaggggata cttgtatttt gctatgggtt
     5821 tttcattagc agttgaatca ctcaacttga ttaaaaatga acgtgtaaag cgtaaaaaat
     5881 taaaaagaga gtaagtatgt tatttgttaa agcttttcat attatcgcct tggtagcctg
     5941 gttcgcgggg ttgttttatt taccacggct ctacgtatac cacgctgaat cactagataa
     6001 aattagcgtt gatcggttta aaataatgga aagaagatta tattacggaa ttacatggcc
     6061 tgcagctctt gcaaccacat ttcttgggtt gtggctattc acttacaatt tatcttatta
     6121 tcttaaagca gggtggatgc acatgaagct tgcattggtt ttgctcttat gggtatatca
     6181 tttaacttgc ggtcacttcc ttaaatcatt cgcccttgga aagaatacaa gaagctctcg
     6241 tttttatcga ttttttaatg aaatgcctac attgctgctg gtaggcattg ttttgttggt
     6301 ggttgtaaaa ccattttaat ccatttcaac catttcgaaa tcttcttttc cagcaccaca
     6361 atcaggacaa aaccagtttt caggaacatc ttcccatcgt gtgcctggct caataccatc
     6421 ttctggccaa ccttcagcct catcataaat aaaaccacaa ataacacaaa tgtatctttt
     6481 gtactcttgc atagtctaaa ctcaataaaa aagtgctaat ttatctactg ttgatgatta
     6541 tgtaaagtta aaccttattt aatatcacaa taattttaaa ttaaacctta taatgaacat
     6601 tataacttca tcgagaattg acatgggttt gctaaaatca accccaaaaa ccagtcaatg
     6661 tggttatctt ttaaagacag gcgctgccac tattttttgc aatattcatg aaatgattgt
     6721 aatattttct tgcctcaggg ttatcagtct aatttgatta gatagttttt aataaaatca
     6781 ttatttcgtc aatcagaaag agtaccagta gaaaaatatt ttaacatttt ttgtataata
     6841 aatttctgaa acatcttgaa tattaaaaat aataactgat atttatctaa agtttatcat
     6901 ttttgggatt ttctatgagc cgttcttcag atttatttca taaagcacaa acaatcattc
     6961 ccggaggtgt taactcaccg gttcgagctt tcaaaggtgt tggaggtgaa cctgtttttt
     7021 tcaaatctgg caaaggtgcc tacctgacag atgtagatga taaacaatat attgattatg
     7081 ttggatcatg gggaccactt atcctcggac actgccatcc caaggtgata gaagctgtgg
     7141 ataatgtatt acacagtggt atgagttttg gtgctccaac agaactggaa atacaattag
     7201 ctgaaaaaat tgcttcccta atgccatcga tagaaaaaat tcgcatggtg aactcaggaa
     7261 ctgaagcaac tatgactgca attcggcttg cgcgcggatt caccaataaa aataaattta
     7321 ttaaatttaa cggctgttat catgggcata gcgatagcct attggttaaa gcgggatctg
     7381 gtttgcttac tctcggaatt ccttcaactc caggcattcc acaaagcatt accgaacata
     7441 ctttaacggc tgattttaat aacctggaac aagttgctca actctttgaa aaatatccaa
     7501 acgacatcgc cactgtaatt ttggaacctg ttcctggcaa tatgggattt atcctaccta
     7561 agatagagtt tttaaaagga ttacgtgagc tttgcgatca atataatgct ttgttaattt
     7621 ttgatgaagt aatgactgga tttagagttg gtttacatgg tgcacagggc ttgtttggga
     7681 ttaaacctga tatcacaaca cttgggaaaa tcataggtgg tggtatgcct gtaggtgctt
     7741 taggtggtaa acgtgaaatt atgtcctttc tggcgccaga aggccccgta tatcaggctg
     7801 gtactctttc aggaaatcca ttggcaatgg cagcaggttt ggctacttta aaagagattg
     7861 aaaaaattaa tttttttgaa gatttaagca atacgactaa taagttaact gaagcattag
     7921 ccgatgcggc agaaaacgca aacattccat tttttactgc atcattaggc ggaatgtttg
     7981 ggttttgttt tacagataag aatagtgttg aaaattattt ggatgttgct tcctcggatg
     8041 aagttttatt taggaaattt tttcatgcta tgcttgctca aggagtatat tttgcgccat
     8101 ctatgtatga agctggattt gtctccagta tgcatggtga tttggaaata caaaaaacat
     8161 atgatgctgc tgaattggta ctgaatcaat taaaatctgc ctgatagcag aattgtacct
     8221 tattacctta agttacttaa atacaatctt taatgggtaa ttaagttcag taaagcgtct
     8281 aaatgtgatt gcagaacaga tttttaaact ggttaactgt aattttaaat aattaataaa
     8341 acactagaca gctgtttttc cctatgtcat attccattct ctagatcgca agtatggaat
     8401 cttggtgaaa aatcagattt gcgcagaata agtaaaaagt ttctaattgg acctaataac
     8461 acaactgcag tttgattatt aaaatatatt taattcattt tagatacttt ttcacttgct
     8521 gacgaaaggt ggtttttctc tgatttgtcc acaagggtta gtgttaatat tcaagaagag
     8581 ctcaactagc aagggaaagc atataatgtt gattttgagt aaaagaagag gactatggag
     8641 ttccatgccc caaggtacat ttgcattatt ttttatacaa gtggtttcta ctttaagctt
     8701 tagtgttctt tattcaactc ttgtattgta catgaaaggt aaacttggtt tacccatagc
     8761 cacagcaaac agcatcatgg gggtatttat agcctacaat tttgcgctgc atctgttagg
     8821 tggtttttgg ggtggcagac tgctctctaa tcgctcctta ttttgtgttg ggatgatcgc
     8881 gcaaattata ggatgtgttt tgctttctat aggaaataca acttttttat attatggctt
     8941 ggctgcattc ttaacaggtt cagggttaaa tgtaacatgt gtaaattgta tgctgactca
     9001 gcaatttgca cctgaagact ctcgaagaga aaccgcattt ctctggaatt atgcgggaat
     9061 gaatattggc ttttttattg gatttagcct cagtggggcg ttccagattt ctcaaaacta
     9121 tcaaaggcta tttcttttaa gcagtttagg taatcttgtt gctttgctta tatgcctcta
     9181 tttctggcaa caattacatg ataaaaacac tacttactca agaaaggaca aacctgcaca
     9241 aagaaaatca acagcattgg ggattgggct aatttttgta cttccattta ttttaagaca
     9301 attattgcag tatgctgatt gggccaataa attggttatt gtcactggaa ttgttatgct
     9361 catattaatc ttgatattag cccaacaaca atctacaaaa gaagcaagag acaagattta
     9421 tgggtttgca gtattaatgt tggttggtac tgtattttgg atgttatatc aaattggccc
     9481 aatggggtta accgtattca ttgatcataa tgtgcaacga caatatggta attggattat
     9541 tccgccacag tggtttcaaa atattaatac tgtagtcata gtattcggag ggccactatt
     9601 gggtttcatc ttaaatagaa tgcgtcaaaa tggatctcag ataaatattc ctactcaatt
     9661 tgctttcgca ttatttttta taggggttgc ttttgctatc ttaccaatag gtatcaatta
     9721 cgctaatcag catggtatgg ttaatccggg ttgggtagta ataagcttta tattacaaag
     9781 tattggcgaa cttctaatat ctccaattgg ctatgcgatg ataggagcgt tggccccaac
     9841 ctcactccag ggggtcatga tgggaatgtg gatgctgaat actggagttg gtgccactct
     9901 atccagttat agctcaaact taatgattat tggtcaagaa agtacttctc ctttattaac
     9961 caatgagggt tacagtcatg tctttttgat gcttggtttg tttgcaatag gatcatccgt
    10021 tatccttttc attttagtgc ctaaactaag aaagctgatc aaagaaaaaa acttgaatag
    10081 ctttaaacag tctggcatag aggtaactga aagagtagct ttatgtgagt agtccctttg
    10141 tcccaattat aacggcagat attaattggc gtgatgatct gccgtattcc ttacaatttg
    10201 atgatattta ctattctgct gaaggtggaa ttaatcaaag tctttatgtt tttgtcgaag
    10261 gtaataattt gattaatcgt tggcaacaat taccaaccaa tgaatcaaac gtatttacaa
    10321 tcgctgaaac tggtttcggt acaggtatga atttcttatt aacatggaaa ctatgggaaa
    10381 aatttgcacc tcaaaatgcc cgcttacatt atatttcctg tgataaacat ccattaaaaa
    10441 aaaatgacct aatcaaatgc ttacaaaagt ggccagaact gagtgtgcag actgaaaaat
    10501 taatagagca ctatcctgtt ttgacacctg gttatcatca tttagcattt agtaacaatc
    10561 agattacttt aacattaatg ctaggagacg tgctggagtg ctatgagcaa ttattattct
    10621 gtggggacat taaccttgaa catcaattga gagaatccta tgttaatgct tggtacctgg
    10681 atggattttc accatcaaaa aaccaatcca tgtggtctga caatctgttc actgtcattg
    10741 ccatgctatc taaagaaagc accacagtag ctacctattc tgcttccagt atcgtcaaaa
    10801 ctgctttaac caatgcagga ttcgttattg aaaaaagaaa gggatttggg cccaaacgac
    10861 acatgatatg cgcccattat gaaaaagctt attcaagttc aaaaaaaaat cgtcataccc
    10921 cgtggcacat aaactatcct gtgacaaagg acgaaagaac agcgctcatt gttggaggag
    10981 ggctcgcagg ttgttttatt gcaaatagcc tcgctaagcg tggatgggaa gtgaccatat
    11041 tagaagaaaa agagaaagtg ggatgtggtg gatcagcaaa tcaacaggct gtattgttcc
    11101 ctaagttatc cacctataaa tcaccattta ctcaatttat gctttattcg tttctttatg
    11161 ctaatgacgt ttacaaggaa cttttgaaac attatgacct tggtgaatta aaaggctctt
    11221 tgttgttagc gcataacgag agggaaaaag caaaccagca aagtcttatt cattggctag
    11281 aattataccc tgaattaggg caattagtgg atgaaaagca atcttctgaa ttatcaggga
    11341 tatctttgcc atgtggtggt ttatttattc catcttccgg ctggattaat tcccctgaac
    11401 tatgcgacat tctaattgat aataaaagaa tttctctaat cactggcaat agagttcaat
    11461 ctattaacta taatcaaaaa aactgggtag ttaacgacat cgaagcctcg gtactaattt
    11521 tggcaaatgg acagcaagta aattattttt acgaaaccaa tcatttgcct gtaaaagcca
    11581 ttagaggaca aatgactacg attcaatcaa cacaagaaag tatcaaattg aagattcctt
    11641 tatgtgccga agggcatgtt ttaccagctt taaacaatag tcatagagtg ggagcaagtt
    11701 atgatattgg cacatctgaa cctgaactta atgctctgga tgatcaatta aaccttgacc
    11761 gattaaaacg gatagcgcct gatataatgt ggtcccaaaa cgtgttagac cattgggctg
    11821 gtataagagc tgcatcacca gattacttgc ctatagtggg acctctcccc aatgcgcttg
    11881 aatttaaaga aatttatgca gaattaaaat ccaactccaa acgatggata gctgaagcag
    11941 ctccttgtta tccaaatcta tatgtgtgtg cggcttttgg gtcaagagga ttaacaacaa
    12001 tacctctcgc aactgaatgg ttagctggtt taattaataa ggaaatttca atactgccca
    12061 gaaaattgat acaagctata tcgcccgcac gattcttaag aaaaaaaatt atacagggac
    12121 cataagagat gtattgaaga tatattgata attcgactta acttcatctt tctttatcag
    12181 ttataatttt aatttttatg tgagctgatt gtgcagaata ttgagtttta tcaaatagat
    12241 gcttttacgg acaaagtctt ccatggtaat ccagctgcgg tttgtattct cgatgattgg
    12301 ttaaatgatg agctattaaa agccattgct gcgcaaaata atttggcaga aaccgctttt
    12361 attataaaag atgatcaaga ttttcatatt cgttggttta ctcctaatgg ggaaattaat
    12421 ttatcaggac atgcaacttt ggccgcaggg tttgctttac ttgagtcaaa taaaggacct
    12481 agcgattcta ttatttttca ccactccagt ggacagattt cggtagaaaa gagaaaggaa
    12541 aaatttgttc tgaattttcc aagaataggt tataagaaaa ctccttcacc agacccaatt
    12601 gtatcactct tggatcaacc agctatagaa gtatttgaaa cagaccttga ttatctggtt
    12661 ttgcttgaac atgagtcaca gattcgaaat ttgcaaatcg atttgtttgc tttagcaaaa
    12721 ctccccaaaa gaggaatcat tgtgacctca acaacaacag aagccgattt ctattcccga
    12781 tgtttttatc caaaacacag cattgcagaa gatcctgtta ccggctcagc acattgcata
    12841 ttagctcctt tttgggcaga aaaactcaat aaaaacagcc tatatgctat ccagggatta
    12901 cacagaaaag gggagttata ttgtgaggtc cagaggaata gggtattgat ttccggccat
    12961 tgtcgatggt atctacgcgg gcatttagta atttaataaa atcgattgag atgcattcat
    13021 cattgaagca taatataaaa cccatttagg aaccaatgga gcatgataac aatatctatt
    13081 ccaaaaggaa ttctctccaa tagaataagg taaaatagtc aatcgcttca aagttcagta
    13141 ataataaaaa catacgcagc aattttgact gcactagaaa taaaaactag accaactgat
    13201 ggaggaaaaa aatgagtagc aaaagtggag gtttagccgg tgtagttgct ggtcaatcag
    13261 caattgccac agttggactg gcaggcaaag gattaaatta cagaggctat tcaataaatg
    13321 atctggctga atatgcaagt tttgaggaag ttgcttatct gcttcattat ggtgaacttc
    13381 ctacccaaaa ggaacttgat gtatatataa aaaagctggt taacctaaga catatacctg
    13441 atgtgcttaa aacggttttg aaacttatac ctaaaaatac tcatccaatg gatgtactgc
    13501 gaacagcatg ttcttttttg ggcactatcg aacctgaaga gaattttaag caacaatatg
    13561 aaatagctga tcgtttgcta gctcttttcc caggaataat gtgttattgg tatacatatc
    13621 atttcaaaaa taaagaaatc tctggcttaa gtgaagaaga cacaacagga ggacactttc
    13681 tggcgttatt acacggacgc aagcccagca aaatagaagc cgatatgatg aatgtctcat
    13741 tgatcttgta cgctgaacac gagtttaatg cttctacctt tgctgcaaga gttacagcgg
    13801 caacactggc agatttttac tcagcgatca caagtgctat aggaacattg cgtggtcctt
    13861 tgcatggtgg cgctaatgaa gcagctatgg agttaattga gcgctttaaa acccctgatg
    13921 aagcagcgat aggcataaag caaatgcttg ccagcaaaga actgatcatg ggatttggac
    13981 atcgggttta tacaacttgt gatccacgct cagatattat taagaaatgg tcatttagat
    14041 taggtgaaga aaaagacaac ctacttttat accatatttc agaacgtatt gaagaaatca
    14101 tgtggcaaga gaaaaaacta tttcccaacc tggattttta tagtgcatcc gcttatcatt
    14161 actgcgatat accaactcat ttgtttaccc caatttttgt gatgtcgcgc atcacaggat
    14221 ggtctgcgca cgtatttgag caaagagcaa ataataaact aataagacct acttccgaat
    14281 atataggccc agattccagg ccatttcctg ctattgaaac acgaggttaa tatgcacaat
    14341 tatgtagagg ataatgttaa gccagactat gatcaggtta taacagatat agctgatttt
    14401 gtattaaata aagaaattga tagtgtgcag gcctatgaga ctgcacgatt atgcttaatg
    14461 gatacactcg gttgtggcat attggccctt aactttcagg aatgtaccaa attaatggga
    14521 cctatagtac cgggagcaac tcttcctgga ggcgcgagag taccaggtac taattatgaa
    14581 ctggatccgg ttcaagctgc ctttaacatc ggtacgatga tacgctggct agactttaat
    14641 gatacttggc tcgctgcaga atgggggcac ccctccgata atttgggtgc tattttggct
    14701 gttgccgatt atttgtgtcg ggaaaaatgt aatcatggac gtcctcccat tttgatgcat
    14761 gatgtactta cggcaatgat taaggcacat gaaattcaag gttgtttggc cctggaaaat
    14821 agcttcaatc gcgtcggcct agatcatgtc atattagtta aagtagcgag tgccgctgtt
    14881 gctgctcact tgtttggagc agatcgtgat atgatgctaa gaactctctc gcaggttttt
    14941 gtagatggac aaagcttgag aacatataga catgctccca atgcaggttc aaggaaatca
    15001 tgggcggcag gagatgctac ctcccgtgcg gttcgtttgg ctttaattgc caaggcaggg
    15061 gaaatgggat atccaagcgc cttgaccgca caaacatggg gattttatga cgtgctattc
    15121 ggtaaaaaac aattcaagtt tcaacgccct tacggcagct atgtgatgga aaatgtgtta
    15181 ttcaagttat cttatcctgc tgaatttcat gcacaaaccg ctgtcgaatg tgcagtaaaa
    15241 ttacatccta tagtaaaaaa taggttagag gacatttcta gaattgaatt aatcacacat
    15301 gagtcagcaa taagaataat aagtaagcaa ggtgctttat ataatcctgc cgacagagat
    15361 cactgcttgc aatatatggt tgcagtagga cttctctttg gtgatttaaa ggcagagcat
    15421 tatgaagacg acgttgccgc agatcccaga atagatgctt tacgagctaa aatgcatgta
    15481 actgaaaatg aacagttttc acgcgattat catgatccgg aaaaacgctc tatagctaac
    15541 agcatgcgaa tcatctttaa agatggctca gaaagtgaat taataactat agaataccct
    15601 attggtcata aacggagacg cgaagagggg attcccgttt taatgtctaa attcaagcgt
    15661 aatctgagta caagattctc tactgacatg gttgaaaaga ttattcatgc aatgagcgac
    15721 accaacacgc tatcatctat gaaagtaagt gattttatgg ccatgtggat ggtaaattaa
    15781 aaagactaac acccacgcac aatctattat ctgtactgat tttagcacag accaggaggg
    15841 ggctaagcgt atagcaaagc ccaccctcct tactaaatcg tagccttcaa acataggttt
    15901 tatattttcc tttgtaaacc ggcaatagca agaacctttg tcgatatttc ttctatggaa
    15961 tattttgttg aattgatata aggaatattt tctcgttggt acatggcttc tacttctgtt
    16021 acctctaatc tgcactgctc tgcggaggca tatttgctgt ttggtctacg ttcagagcga
    16081 atttgctgca aacgttgtgc gtcaattgtt agtccaaata atttttgttt ataaggtctt
    16141 aatacctctg gtaagcgaaa acccaccaaa tcttcttctg taaaaggata attggcagct
    16201 aaaatgccat attgcagcgc catatataag caactgggag ttttgccaca acgtgaaact
    16261 cctataagaa taatatctgc tttatcgtaa ccacgcgttt ttattccatc gtcatgtgaa
    16321 agggcaaaat caattgcttc aattcgatgg gaataggatt tcacattcgc aactccatga
    16381 gtcctgccta ctgtataaga tgatttctct tcaagctcat tttccaatgg cccaataaaa
    16441 atactaaaca aatcaaaaac acaggcatgc gcctttttaa tggcttgtct aatttctgga
    16501 tcaactaaag tcatgaatac taagggcttg gttccttgct ctgaaaaaca ttgattaatt
    16561 cttagtacaa cactttctgc tctgtgagtt gaatctatgt agggtattgt tattttttca
    16621 aattgaatat tctcaaattg agtaatcaga ctattaccta atgtctcagc cgtaattcct
    16681 gtaccatcgg aaagcataaa aacataacgt ttcataagtg gcctttttaa ttcattgatt
    16741 aataaataaa taatatttaa attataaatg gaaatagcga ttgtacttat taatagccat
    16801 ctgctatctt aaaaataata atattacaaa tctaatcatg ggaacactat gcagcataat
    16861 atatacgaga atagtaaact acatattcta gggattatct gccttgtaac ttccctgggg
    16921 ctattcttct ttagccttta tattgtacct ttcttaattt ggcaattgga ttacgatatc
    16981 cccgatttta ttagtaacat gatagcatac ggggaagatg agtattactt cagccctatt
    17041 actagtaaaa cactggtttg gcttatcttt tttacaccaa gtataattat gggttatctt
    17101 tcttattaca tctctaacta tattgataaa agctatttag aaccccagga aaaaccttta
    17161 gaaagtatgc aagaaaaatc accttccaaa cctgctggtc gtgggagaga ttcgactata
    17221 ttgggattga aaatcgttct cttaatggtt ttaattgttg caataatttt tttactacaa
    17281 atattatttg acctaacagc ttaggtgagg gagtagggca tgtttatctt taaacgatgg
    17341 aaaataagaa aaatcacaaa aagaatcaag gctatgcaag ccaatagagt aagtaatcaa
    17401 cctggggatg aagttcttaa aaaagaaatc ctttattatt ttgaattagc tacaattttt
    17461 aaaaaattaa agaatcataa aaaataccct tacgcagaaa tcatgatgat tgagtgttat
    17521 agagcagcag ctaatctcga tgactcggca gctaattttc agctaggcca aatttttctg
    17581 gacgaagcaa aatacaggca aaaactggat aatgaaggaa tatttaatag tcaagccaac
    17641 ttaaaaagag cacagcaact ttttgatgag gcccatgcac atttaatcgc agctgaaaaa
    17701 ctggggcatg taggtgccaa gagattaaga ggactatgta ttatcaacgg ctggggtgtt
    17761 gaaagcgata aaaatgctgg attcgagttg gttgtcgaca gtattgaaca agagggaagt
    17821 tgggataaaa tacctcaaat atttgcatca atgggattaa ataagccaga atttttctca
    17881 gctattatgc aaagaagaaa aggaacatct tgaaatttat tcattattaa ttccctaatc
    17941 aaaaattatt tatgaggtat atattgtcgt tgcattaaat aaagtaaatg tggacttgca
    18001 tatttaggat tgaaatagca atgaaccaaa atgatgcttc taaaaaagat aatggcaact
    18061 ccaatcaatt ggatactgaa ttaataggaa gttaccaatc gataacgatt ggtttctcaa
    18121 aagatgaact tgccagtttt tcaaaaattg gtcagcataa acactatgct gctggaagca
    18181 ttgtttataa tgaaaatgat ctcgctgact cattctttat tataatcgat ggcgttgccg
    18241 aaatatttaa aaaaactatc gatgagtttg gtgagcataa tatccatatt cttgcaacct
    18301 tatcaaaaga tgatgttgta ggagaaatgg ctctcataga aaatagtcga cgttctgcgt
    18361 cggtaaaggc taaaactgat cttagtgtta tagaatatcc attagaagtg gtaaaggcta
    18421 aaccaaagct taacttgatg ttaattaaaa atatggctca aatcctttcc aggagacttc
    18481 gttttacaaa tgaagtaact gtgaaaaata tgcagcaagc tctcaaagaa gcatcagggc
    18541 gcaatacatt gggggtattt atcatagctc tactatggat tatttgcctc tatactttat
    18601 ctctacattt cctggttact gtaatacatc aggtaactgt cactaccgta ctatcaatct
    18661 gcttaatctc aatctttgct gtagcaattc tcattgcaat gcactttact ggtctcccat
    18721 attccagatt tggtatcact gcgaaagatt ggggaaaaac catcttggag gcattagctt
    18781 ttacaattcc tattatgctt ttcgtactaa ttatcaaggt tttttttata ataggtatcg
    18841 atcatctgga caatactcct atattttcag gaacaaaacg ctttttggta aataatgaag
    18901 tggattggga atattacttt gctatgattt ttttatatag cctttttgcc cccattcaag
    18961 agctcatggc cagaagtgcc ttacaatcta ctttttttca ttttttaccc ggggaaaaat
    19021 tatttcgtca atggaatgga atttttctgt ccaatctaat ctttgcaact atgcacactc
    19081 acttggggct aacctttgcc tcattaactt ttatcgccgg acttttttgg gggtggttat
    19141 ttcacagaca gaaatctctt ttgggagtat ccgtttccca tgtgatttta ggcgtgtggt
    19201 ctctatttat tgttgggttg cgctaagcta atcaacattt tacatccaat aacctatata
    19261 ccaattgata attgagtctc cccaaaacat agctattaat ccagaaatgc aaagaaaagg
    19321 gccaaatggt attgcagtat ctcttgattt accatttatc tttaaataaa tcaaaccaat
    19381 gatagccccg gttattgaag atattagtag aattaatggt aaatacatcc atccaagcca
    19441 tgcaccaaat gctgcaaata atttgaaatc cccatgtccc atacctactt tacctgtcat
    19501 cagataaaac agattaatga ataaccataa ggctaaatag gcgccagcac agcttaatac
    19561 agcaacgcca agactaacaa atatattctg ggtattagca ataaggccaa tccataaaag
    19621 ccctaaagtg aggctatcag ggagtaattg atgatcaaga tcaataaaaa caagtgatat
    19681 taatatccag atagctaata aagcaaataa tagttgtatt gtaaacccaa aatgccaact
    19741 ggcgtataag gataaaactg tggtcagagt ctcaacaaaa gggtagcgaa ttgaaattgg
    19801 actatcacat tggtaacagc gtcctcttaa caaaatatag cttaacagag gaatattctg
    19861 ccacgcttta accatcgctt tacaatgagg gcaaaaagaa cgtggtaaaa ataaattaag
    19921 ttttattttt tcttttcgct gttcaagctg gaatagctca cagcattgct ctttccactc
    19981 ttcctgcaaa ataattggta agcgatagat tatcacatta agcaaacttc caacagctag
    20041 agaaaagagt ccaacgacta aatacataaa ccatggataa ttgattatta gtgcatttat
    20101 cattgacacg tttgcctata ttgtatgatt aaattaaata ggatctgttt aaacagcaga
    20161 acccagtttg aaaataggta aatacatagc caccaccaat cccccaacca gtatacccaa
    20221 tattgacata attatcggct ccaataaact acttagggca tctacagcat tatttacttc
    20281 ttcctcataa aaatcagcta ctttgcttaa cattttttca agggcacctg attcctcacc
    20341 aattgctacc atttgaatta ccatattcgg aaataaatga gtgttttcca tagcaataaa
    20401 catttgctgt cctgttgcaa cttcttccct gattttatca gtcgccttgg cataaataat
    20461 attcccggtc gctccagcta cagatttcaa tgcttcaacc aggggtaatc cagcagcaaa
    20521 agtaatggat aatgtcctcg caaatcgagc aattgctgct ttctctaaaa tagggccaat
    20581 tacagggaat tttagcatca ctctgtcaat aatttgagca aattccaaag agtgattttt
    20641 agcataaaaa aagctataga caatcccacc caaagctcca aaaataatat accaataagc
    20701 ctgcataaat tcagacatag tgatcacagc tctagtcatc gctggcaagt cagctccaaa
    20761 tcctttaaac aatgattcaa attgaggaac cacatagatt aataatcccg cagtaaccaa
    20821 aagtgcgaca accataacag caattggata agtgagagct ttttttattt tcttttttat
    20881 gatttcaatt ttttctttat acgttgcaac cttgtcaagc atgatatcta atgagcctga
    20941 tttttcacct gcatcgacaa gattacaaaa taattcatta aaatagagtg gatgtttaat
    21001 taaggattct gctagcgtta gccccgtttc gatatcgtgc ttaattgttt ctattaagtc
    21061 tttaagtctt ttattgcttt gtcctttggc aacaatatca aacgcttgca ctaatggtat
    21121 tccagactcg atcatcgtcg ctaattggcg gctaaataca gtaatatctg cttgtgtgat
    21181 ttttttattt tttctatcaa acaaaggttt tctttttttt actactttat tggtaacaat
    21241 cccctgctta cgcaaatcag ctttagctat tgccaagctt ctggcttgaa tatcaccctc
    21301 cattttttga ccggctttat taatgccttg ataatgaaag gttaataggg gtgggctatt
    21361 tttatccatc caaattaatc aacggttacc cgattgacct cctctatggt cgtgattcct
    21421 tccttaactt tttctatacc cgattgaaag atagtcagca taccttctga ttgagctaac
    21481 tttaatatat ccagcgaatt accacctgac ataatcaatt ggccaagctc tttcgtcatc
    21541 ggtaaaacct caaacaaccc aactcgcccg cgataaccac ttgtacattg ctcacaacca
    21601 actgctttgt ataatttaag attaaccaga tcagtttcct tgaaacctag ttcaatcaag
    21661 ccttgattgg taaagtcatc tcgcactgcc ttacattgat tgcataattt tcttgccagc
    21721 ctttgcgcaa taattaaggt aaccgaactc gcgatgttaa aagtaggtat tcccatattt
    21781 actaaacgat ttaaagtttc agcagcactg tttgtgtgca gagtagaaag aaccaaatgc
    21841 cctgtctgag cagcttttac agcaatctct gctgtttcaa gatctcgtat ctcaccaacc
    21901 attataatat cagggtcttg ccttagaaac gaacgcaatg caccggaaaa ggttaggcca
    21961 gctttaggat taatattaac ctgattgata ccaggcactt tgatttcaac cggatcttca
    22021 gctgttgaaa tattaacctc aattgtattt aatatattta aagccgtata taaagtgact
    22081 gttttaccac taccagtagg gccagtgact aaaatcatcc cttgtgggcg ctggattgct
    22141 ttcaggaaat ttgttcgctg aacaggatta aatcctaatg cttcaatacc taatttagca
    22201 gcaccagaat caagaacccg cataaccact ttttccccag ccgaggttgg acatgtactc
    22261 actctgaaat caattgctcg tgacttggaa attttcattt tgaagccacc atcctggggg
    22321 atacgccgtt ctgatatatc caaattagac attaccttga tgcgtgctgt aatacgagat
    22381 gacaagcttg cgggaggggt ggcgacctca tgtaaaatac catcttgcct atacctgatt
    22441 cggtattctc gttcataagg ctcaaagtgt atatcagaag ctccttgcct tatcgcatcc
    22501 aataaaattt tattgacgaa ttttacgata ggcgcatcat cagttactga tgttgcagta
    22561 tcaatatctt gatcttcatc atcagcactt atttctaaac cttccagatc tccagagtct
    22621 tcaacatact ctgataagcc ctgactttcc tttgctgtta acaggttatc aatcagagca
    22681 ctaagtttat ctgtttctac aactatcgca tgagtattta atccggtatg aaattgaatt
    22741 tcctttaatg aagcttgttt actaggatca tctgttgcga gatataaatt ggtgcctcga
    22801 ctaaaaagag gaaccatggc atgacgttta attaatttct cattaaccag attggcagga
    22861 atggtgccca catcgatgca attaatatcc aacatgggta cgccaaaatt ttgagcagcc
    22921 gtcagtgcaa tttgttcagc agataatatt ttatttttta caatgtattg cagcaaagat
    22981 atcttttctg ctgctgccaa cttgtgtagt tcaattgctt ttgttttatc tagcagcttt
    23041 tccagcacga gaagctggcc gattccctgt aatctatatt cttctgtagc tagagccatt
    23101 tcatttattc aaagtgtatc ttagttttaa atatagttaa atttttttat aattgcatgg
    23161 ttttttgaac aaaacctggt attttggacc tgcccctaaa tcctatttct ggttactttg
    23221 ccatatcaat tttttgc
//
LOCUS       DASLOE010000033        22897 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.033, whole genome shotgun sequence.
ACCESSION   DASLOE010000033 DASLOE010000000
VERSION     DASLOE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22897
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.033"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1017)
                     /locus_tag="U6309_001046"
     CDS             complement(<1..1017)
                     /locus_tag="U6309_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEN5504499.1"
                     /translation="MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNID
                     SKNIPYIAWWRDFKDPTLNQLIERGLISNTSLGMSRGSIEAAEGELKKIRYQWVPTLD
                     LMTGYSRNPATGFPGVLIVFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIIS
                     QIAASYFTYLAEIERKEFLQTLANDLTQLAKVASKVYQGGLSSEIEQEELYSQVNLIQ
                     GELEVTERNIVISRNALRYLLNQNPGEIKTVKKLLQLKNRKLIPGSLPLTVLENRPDM
                     QVAENRLRASHEGIGVAESNLLPTIQLDYIGGLAAGDSRYALPKKTVKFNDQLLKAPI
                     IDMPV"
     gene            complement(1001..1906)
                     /locus_tag="U6309_001047"
     CDS             complement(1001..1906)
                     /locus_tag="U6309_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN5504500.1"
                     /translation="MRRWLGVQAFSYVELRSIQISTVFAFTIFVQEWLRYPRAGWTGF
                     AVMMIYAGFDNGTTIFRTYHRFLGVLLGLFTGYLLWFVGHLDYRTLILIMPLMVFFAF
                     FLVGRAYSVPTVFTVNVSVLGSGYFSTEDTLPVTYFIIDYTLCTLIAFTIILGFEYFW
                     FRHYGLMQRFIKSTQAEVIDDLYRLVRLLNQGKIKKTEWFQACIKLTDSLFEVNKLIT
                     NSQFLISSEHAVGDEFNQFVELANRIFIGLKALYMAYYTKHYHTFDYYQLFQQVQKDL
                     VRLKDFVAGEQTIDLSEGVKHATSG"
     gene            complement(1891..2823)
                     /locus_tag="U6309_001048"
     CDS             complement(1891..2823)
                     /locus_tag="U6309_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN5504501.1"
                     /translation="MFISQLKDKLASYDRYKEHRINGLKAVFVLELMIIFYFFSSIDN
                     PYFYYFYAPITCFTVEAAGTTLKEKYMLLLYTLLGSILSIFLFGIISVYNVLFIFFVF
                     FYTLALYFLVLHHFKKMLVIVPLMLSLASYSLIYGVEADSNFYIAINNALYTLAAMAV
                     IFAGLFIFPKKYYFDIWERAFLEVITGLESLSAKICKGEVKTIAIFSGIIVMERYSKM
                     LPRNIKCYSILKITILSFELILTMSYLLSFQKQLRKEYVIVLHHYLERLCQSCKARQP
                     LILSDQELPAFKETHELEILYQLILSWNYLCADG"
     gene            complement(2798..3871)
                     /locus_tag="U6309_001049"
     CDS             complement(2798..3871)
                     /locus_tag="U6309_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEN5504502.1"
                     /translation="MKEKFATWYKIVTKHIRFFFSYSIPMFFKKAKNYINLPIIVVGL
                     ALIIGLFHLFSYLIPFTDNAFVVTNVTPVAADVSGFITEIYVKNGQKVKKNDPIFSVY
                     RVPYELAYQQAMADYKEGLKKIDVFQKQIERTVALIKATDAELEKAQFALGLKKNKNV
                     AEAVSVLELKNLGYDVSTLSNKRNALQQEVAVLQAKIEQQRHTVASLKAKMDNAKVNL
                     DLTIVRAPGDGVIDNMYVSPNTPIEIHKPVFSFIDTANYYIQANFNETDLRNVRQGDK
                     AYIILRMYYFDKIFHGVVVNSLWAAERQSTAPKSQIQVVTNQNEWLLLPQRFPLQIKI
                     LDPDPDYPLNPGASAYVYISTKR"
     gene            complement(3972..5183)
                     /locus_tag="U6309_001050"
     CDS             complement(3972..5183)
                     /locus_tag="U6309_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN5504503.1"
                     /translation="MSLEVEPKPHGNNPYANRFDNLPLKDQGRFPRVAYVLQGGGSLG
                     AYQFGVVKGLLEAGYEPDWIAATSIGAIQAAIIVGNPPEKRVEKLERFWDEIAPGNFF
                     DFLGESDITLDFYNKLSAACALLFGQPEFFFPKWCTGTIPLWGDPTTLSYYDTTPLRK
                     TLLDLIDFELLNSCPIRLSLGAVQISNGHLIYFNNINYRIEVEHIMASAALPPGFPAV
                     NIDGELYWDGGVHSNTPLEVILEAIPAENTLCFLIDCFGGRSFIPKNMSEIEERRKDI
                     SYGTHAQRTIYNYLQRQKMRNSMMELSRILTDEQKKESAHLLDIGSPHHCTLVHLAYS
                     SRIVKAASKDYNFGRVVIKKRMEAGYSDAKAVLAEESQWGYLPEDGKSRLYESPNNLS
                     RLFRKRKKEVF"
     gene            complement(5620..6459)
                     /locus_tag="U6309_001051"
     CDS             complement(5620..6459)
                     /locus_tag="U6309_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEN5504504.1"
                     /translation="MKAMEVEQLIQGTLIRDGGGVKLRRYIGADKTNHFEPILLFDYF
                     DSSDPLDYLAGFPPHPHRGFETITYLMEGSITHEDNKGNKGVINAGGVQWMTAGKGII
                     HSEMPSSSTDRLHGLQLWLNLPAAEKWREPRYQEKSSDQLPIEVMESGATIKVIAGRI
                     DQGLSSPIVEIATRPLFLDITLPANATIQQTIPDDYQAVLFVIEGTVNTGGQQITAGT
                     LASLTNGERIQIEGKDKENRNHCLLIAAAKLHEPIVRYGPFVMNTQEEIMQAIHDFRT
                     GHF"
     gene            6605..8182
                     /gene="cegC1"
                     /locus_tag="U6309_001052"
     CDS             6605..8182
                     /gene="cegC1"
                     /locus_tag="U6309_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector CegC1"
                     /protein_id="HEN5504505.1"
                     /translation="MNTTEHTELGNGLLMDKIEGNPYLRIDEFGVLHLRLQRFGENNL
                     PEPMELEMSAGEIIAMAGDYFTQANWTMDLDLPKCELFNSPAELGKHLIRKPIDPKEE
                     NALITAYNNLAAPDVTRKEIDRIYSINNANYVPFSPTLNFYAQQLMYYFRVKDYGEML
                     VRNQTHFTPWSIRVYILGHAIALRYARLSYELKQLATDRNYQSDNPDLSTLKISLQNK
                     NETLSSNTLLDLANRYHAQAYSIELFTFHYYSDHFATGHMSMIGDLRVVLKERFGVWG
                     NILANNLHDEVNRVGVYTVRPYDPTPNTTEAPSRARGDGKFDTCLNQFNRLACLNGMT
                     ASLKDINQVLSGGVIPEQKKFGGLVHMPDVDFNSRQHQPLLVLSKGKVYYRNNLSQIH
                     IISPSEYEALRENPEKHGYKELTSKWAAFKLVTKLRLFPYLYDGSVMPVSDEKLAEII
                     ADEKRRNPQRAPIPTPSCLPESEPTVFDWRTKASWRNNKDSLDILDGLKKHSILAAKQ
                     THSAIQEEEEVRLNLGV"
     gene            8351..8821
                     /locus_tag="U6309_001053"
     CDS             8351..8821
                     /locus_tag="U6309_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEN5504506.1"
                     /translation="MKRLIPTFIYLLVLAITPFSTHAEALLKDTFGHNIPFSSLKGKW
                     VFINYWASWCETCVDEIPEFNRFYKNHKNEAVALYAVNYDALPLPEQKLLIKKFNINY
                     PNLLKDPAQDLQLGDITGVPVTFVFNPKGQLIKKLYGGQTAKTLEKVIAENRTS"
     gene            complement(8926..10170)
                     /gene="hflX"
                     /locus_tag="U6309_001054"
     CDS             complement(8926..10170)
                     /gene="hflX"
                     /locus_tag="U6309_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEN5504507.1"
                     /translation="MFERPQGGERAVLVQLALPGVDADKALAEFEELALSAKAEVLDC
                     VLGARATPDAKYYVGKGKAEEIAHLVNMLDADLVLVNHELSPSQERNLERLFGCRVVD
                     RSGLILDIFAQRARTFEGKLQVELAQLQHLSTRLIRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRERIRYINKRLEKVRCSRDQNRQARRKASMPTVSLVGYTNAGKSTLFNVLT
                     GEHTYVADQLFATLDPTMRKLELPGSSAAILADTVGFIRDLPHHLVEAFRATLEETQQ
                     ADLLLHVIDISDPNWRETVFEVQKVLDELKVNNIPVIQVFNKIDLQEGWQPKIDYTED
                     SCKVWLSATTGAGLDLLKEAIATQLHGMILIEDIVIKPDQAKLRAQLYQLGAVLKESV
                     DENGDWLMKIRITADQKRRLLS"
     gene            complement(10198..10455)
                     /gene="hfq"
                     /locus_tag="U6309_001055"
     CDS             complement(10198..10455)
                     /gene="hfq"
                     /locus_tag="U6309_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEN5504508.1"
                     /translation="MSKNHLLQDPFLNELRKEKVPVSVFLVNGIKLHGIIDSFDQYVV
                     MLKNSITQMVYKHAISTVVPSRMVKIPAEESSGEEEGTVAD"
     gene            10643..11902
                     /locus_tag="U6309_001056"
     CDS             10643..11902
                     /locus_tag="U6309_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504509.1"
                     /translation="MTFTCDELKGLEHPYEVLGNGDALAENREELNKLSNDEKLVLAS
                     RLVLECPVNELKDFSHAIEAARMPQDDSDTFHSFLFQAYQVKKRIISLLDPRNKNPHS
                     MILEKEFDGELFNNFNKLAIDVLNNNEVAIALRLAETTPAQDRSRVSQNINNIFPQSL
                     FAAKVGHAFAVRRDIERLLLGDRPDQFFSSREFKIDSCIEFASLFNVINDKESSIAGK
                     LALRTPAENRTDVVMKIKGFCAEDSELAIKVQSALALRRDIERNLLGDNPEQFFNSRD
                     FSVDLCLEFAILFPELLKGHEQAIGEKLAKLDAKVRSDISRKLEMINGAAHEQSSPFR
                     IIASAMVPKEEPCLTKPTPVVLPSTEEIVPKQESPTSLRNRNVLFKEQTEEHKKKNSQ
                     MEPIVSDSEEEEEKQNCWTSFCGLFGK"
     gene            12443..13288
                     /locus_tag="U6309_001057"
     CDS             12443..13288
                     /locus_tag="U6309_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEN5504510.1"
                     /translation="MKENKLTVALVQEQWYENPKEHQDKLASGIFSATQQGAKLVCLQ
                     ELTLSPYFCTRSDVDPAPYMEDIATGPTAQFVSQMAKSNQIHITASLFEKAGYNTAVA
                     FNPQGELIAVTRKQHIPSGEKYHENFYFKPGNSNYPVHTIAGHYLGLPTCYDQWFPEL
                     SRIYGLKGAEILVYPTAIGGEPTAPGFDSQPMWQKVMVAQGIMSNTFIIAVNRIGCED
                     GLSFYGSSFISTPMGEILVQAPRNEPAVLVAELDFSQRELWGRLFPFPQQREPETYHE
                     LVKSR"
     gene            13308..15200
                     /gene="speA"
                     /locus_tag="U6309_001058"
     CDS             13308..15200
                     /gene="speA"
                     /locus_tag="U6309_001058"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEN5504511.1"
                     /translation="MNNNTLSDDNLYNIENWGEGYFRINSKGNIEISKKPGKPGVELQ
                     TIVDAADRAGLHLPLLIRCSDILHDRVHRIYQAFTQAREDTCYAGNYQLVYPIKVNQE
                     QSVVRELLKSPDNQIGLEAGSKPELMAVIGMLGDHQSTIVCNGYKDTSYIRTALIAQQ
                     MGHKVFIVIEKKSELDIILKESARLQIKPTLGVRIRLVSKSAGKWENTGGVKSKFGLN
                     AKQVLELILQLKANDMLDCLQLMHCHLGSQIANIHDIRHCMQEVARYYIELRQLNAPI
                     GTIDVGGGLSVDYEGTRSNKGCSMNYSLVEYATHIMLALKHLCQEAEISEPDLISESG
                     RALTAHHAVLISNITDVEIINKAPVLPEISEDDSHVIRDIYDTYQAITDSSPTEIYNY
                     AAHSLNEAHSLFKHGVISLQEKAKVEAFYTNICMEIKDKLDEENPSEKALLMRINECM
                     AAKIFCNISFFQSIPDAWAIGQIFPVAPISQLNRTLSMHSILQDLTCDSDGTIKQYPG
                     SSCVTTTLMLPSYDINNPYNIGFFLVGAYQEILGNLHNLFGDTNSLDVKLSDDGQFEL
                     DDLVSGDTVTNVLNLAHFDTKKLIQSYEKQLIKSELPKEKALSYLNELRSIFSQLTYL
                     DGNIAQ"
     gene            15203..16243
                     /locus_tag="U6309_001059"
     CDS             15203..16243
                     /locus_tag="U6309_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEN5504512.1"
                     /translation="MNTAPKQYGFYMPAEWYPHERCWMAWPCHHETWSKIGLDKAKMA
                     YARVAKAIAQFEPVTLLVNPGDEDSAENLCKGHNIEIISLPINDSWTRDTGATFLINN
                     ERQLAGVDWIHNAWGGNYADCSLDNLIASHLIKYTEAQYFHAPLVMEGGSFHVDGEGT
                     ILTSKECLLNSNRNPHLSQQEIEQYLINYLGAERIIWLNMGLIGDETDGHVDEIATFI
                     APGKVLCLITKDKEDPNYHRLQENFEILKSSKDARGRTFEVYTVEQPPATYLNGERLT
                     LSYINFYMANQGIVMPAFGYESFDRLAYQLFVQIFPGYQITQIDALDVFSGGGGIHCI
                     TQQQPKSHKLVE"
     gene            complement(16612..19029)
                     /gene="gyrB"
                     /locus_tag="U6309_001060"
     CDS             complement(16612..19029)
                     /gene="gyrB"
                     /locus_tag="U6309_001060"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEN5504513.1"
                     /translation="MSVDASYDSNNIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFE
                     VVDNSIDESLAGYCKEIFVTIHSDESITVKDDGRGIPVDIHKEEGKSAAEVIMTVLHA
                     GGKFDDNSYKVSGGLHGVGVSVVNALSEELHLLVRRHGKVYEQHYRHGVPESPIAETG
                     EANSTGTQIWFKPSAETFSNIEFHYDILAKRLRELSYLNSGVCIHLFDERTQKKDTFH
                     YEGGIKAFVEHLNKNKTPVHPVVFSMTSEKDGIVIELAMQWNDGYQETIFCFTNNIPQ
                     RDGGTHMAGFRAALTRTLNNYIENEGYAKKAKVSPTGDDAREGLTAVLSVKVPDPKFS
                     SQTKDKLVSSEVKPAVESCVAEKFNDFLLENPTAAKSIVAKIIDAARAREAARRAREM
                     TRRKGALDIAGLPGKLADCQEKDPSLSELYLVEGDSAGGSAKQARDRKFQAILPLKGK
                     ILNVEKARFDKMLSSQEVATLITALGCGIGPEEYDPDKVRYHRIIIMTDADVDGSHIR
                     TLLLTFFYRQTRELLERGYIYIAQPPLYKVKRGKQEQYVKDDEALFDYLTQCALDGAT
                     FYPAKEVPPISAKSLEELVQHFRRVEKIIKRYKRRYPSDILKRVAYMPMLHQEDFNNL
                     PKIENWCRQLHLSLQQVDSKTEQYKVDTQKLTEGNQYTPKITVYQHGLENDIPMQVEF
                     FLSKDYKEMVDLGHKLSNLIEEGAYIQRGDKSLPVENFEQVLNWLMDEAKKGQTIQRY
                     KGLGEMNPEQLWETTMDPEVRRMLQVSIEDAVAADEIFTTLMGDNVEPRRDFIETNAL
                     DAENIDI"
     gene            complement(19327..20388)
                     /gene="recF"
                     /locus_tag="U6309_001061"
     CDS             complement(19327..20388)
                     /gene="recF"
                     /locus_tag="U6309_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEN5504514.1"
                     /translation="MILSEVRIHNFRNIASTSLILNPNFNCITGPNGGGKTSLLEALY
                     MLSCAHSFRSREITPIISYGQNQLNVFAHAYDESTISVQKSITDGTQIKLNNQFCCTT
                     SQLAYALPCQVIYSDIFQIIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNA
                     LLKSRATYDHFIPWDQQLSQLANQLDKARNDYFLQWQPKFYQVLSDLTNISCTVEYYK
                     GWDRKNAGQNMEELLQKSFDSDRKKLYTQYGPHQADLIISTEQYRVKHTLSRGQQKII
                     LIALKLAQGQLLDKDCLYLIDDLAAELDDYHQRNLIKYLTQQKGQFVITNLINNNNYD
                     ILPIDSGLFEVNCGAIKNL"
     gene            complement(20385..21488)
                     /locus_tag="U6309_001062"
     CDS             complement(20385..21488)
                     /locus_tag="U6309_001062"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEN5504515.1"
                     /translation="MFELTIKKEDLLTPLLTVSGAVDKKQSLAILSHFLLKLSDGLLY
                     ITATDLEIEMSAQVACDSKNTSGSITVPAKKFIDIIRSLDENASPSIVFDKALVTIKQ
                     NRSTFKLATLPAENYPSSEDECNDVELTIPRLVLLQLLQSTHFAMSQQDVRIFLNGLL
                     LEFDSNIISAVATDGHRMAISRYSCNNIAQTKLLIPKKGVQELLRLLNCINDEQVLLA
                     AGKSHIKLITNQFVFLSKLIEARFPPYTKAIPREQDKCIVIDCASLKRALSRIVILAH
                     EKSKAVLLHLQQGQLTLIANNNEQEEAIETLSAETQGDELKIGLNATYLLDVLTHFSE
                     GQIRLSMSNTESSILIESLVNDNYQYIIMPMKI"
     gene            complement(21502..22860)
                     /gene="dnaA"
                     /locus_tag="U6309_001063"
     CDS             complement(21502..22860)
                     /gene="dnaA"
                     /locus_tag="U6309_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEN5504516.1"
                     /translation="MSTTAWQKCLGLLQDEFSAQQFNTWLRPLQAYMDEQRLILLAPN
                     RFVVDWVRKHFFSRIEELIKQFSGDDIKAISIEVGSKPVEAVDTPAETIVTSSSTAPL
                     KSAPKKAVDYKSSHLNKKFVFDSFVEGNSNQLARAASMQVAERPGDAYNPLFIYGGVG
                     LGKTHLMHAIGNSILKNNPEAKVLYLHSERFVADMVKALQTNSINEFKRFYRSLNALL
                     IDDIQFFAGKDRSQEEFFHTFNALLEGQQQIILTSDRYPKEIEGMEERLKSRFGWGLT
                     VAVEPPELETRVAILISKAEQSNIELPYEVAFFIAKRIRSNVRELEGALRRVIANAHF
                     TGKPITIEFVHEALRDLLALQDKLVTIENIQKTVAEYYKVKVADLLSKRRSRSIARPR
                     QMAMALSKELTNHSLPEIGDHFGGKDHTTVIHACRKVKELIQDDSDFAEDYKNLMRIL
                     SS"
BASE COUNT         7152 a         4616 c         4154 g         6975 t
ORIGIN      
        1 aacaggcata tcaataatgg gggctttcaa caattgatca ttaaatttca ccgttttttt
       61 cggtaatgcg tagcggctat ctccagctgc caaaccacca atataatcta attgtatagt
      121 aggcaaaaga ttactttctg caacaccaat tccttcatgt gaagcacgca aacgattttc
      181 agctacttgc atatcaggcc gattttcaag aactgttaac ggtaaagaac cgggtattaa
      241 tttacgattt ttaagttgta ataatttttt aactgttttt atttcccctg gattttggtt
      301 tagtaaatac cggagagcat tgcgacttat aacgatatta cgctctgtta cctcgagttc
      361 gccttgtatc aaattgacct ggctatacaa ctcttcctgc tcaatttcag aacttaatcc
      421 tccttggtaa actttactgg caacctttgc caactgagtt agatcattgg ctaatgtctg
      481 taaaaattct ttgcgttcta tttctgcaag ataggtgaaa taacttgccg ctatttgaga
      541 aataattgtt agtttgagag catcatcttc tgcttttact tgtgcaagag tatattttgc
      601 tcttttttgc tctcttattt gattgattat gtttattgta tagttaggaa taaaaacaat
      661 cagaacgcca ggaaaacccg ttgccggatt tcttgaataa ccagtcataa gatcgagggt
      721 aggcacccac tgataacgaa tttttttaag ctcaccctct gccgcttcaa tgctccctct
      781 tgacatgcca aggcttgtat tggaaatcaa ccctctttca atcaactggt tgagagtggg
      841 gtctttaaaa tcacgccacc aggctatgta aggaatattc ttgctatcga tatttttaac
      901 attcttgtct tttaccgacc atttattaat tacttcaaca tggggtctgt gataaggctc
      961 actatagcat ccgactaaaa agactgataa taaaatgatt ttaaccggac gtagcatgct
     1021 ttactccctc actaagatca attgtttgct cccctgcaac aaaatctttt aagcgcacca
     1081 aatctttttg aacctgttga aatagttgat aataatcaaa cgtatgataa tgtttcgtat
     1141 aataagccat atataaggct ttcaagccaa tgaaaatacg attagccaat tccacaaact
     1201 gattaaattc atcccctaca gcatgttctg aactgattaa aaactgggag tttgttatga
     1261 gcttattgac ctcaaacaaa ctgtcagtca atttgataca agcctggaac cattctgttt
     1321 tctttatttt accctgattt agcaaacgaa ccagcctgta aagatcatca atcacttcag
     1381 cctgagtact tttgataaat cgctgcatca gtccgtaatg cctaaaccaa aagtattcaa
     1441 agcctagaat aatagtaaag gcgatcaacg tgcaaagagt ataatcgata ataaaatagg
     1501 tgactgggag cgtatcttcc gtactaaagt aaccagaacc aagtactgac acattgaccg
     1561 taaatacagt gggaacactg tatgctcttc ctaccaggaa aaacgcgaaa aaaaccatta
     1621 aaggcataat cagaattaac gttcgataat ccagatgacc tacaaaccat aatagatatc
     1681 cggtgaataa gcctaaaagc actcctaaaa accgatgata agtcctgaat atggttgttc
     1741 cattatcaaa accggcataa atcatcatga cagcaaagcc cgtccacccg gcccgcggat
     1801 aacgcaacca ttcctggaca aaaatagtaa acgcaaagac cgtactgatc tgaatagagc
     1861 gcagctcaac ataagaaaat gcctgtactc ctaaccatcg gcgcataagt aattccagct
     1921 taaaattaat tgatacaaaa tttccaactc atgcgtttct ttaaatgcag gcagctcctg
     1981 gtctgagagt atcagtggtt gcctcgcttt acaggattga caaagacgtt caagataatg
     2041 atgtaaaaca ataacgtatt ccttacgcag ttgcttttga aaagacagca agtaagacat
     2101 ggtcaagatt aactcaaaag ataaaatggt tatctttaat atactgtagc acttaatatt
     2161 tcttggcagc attttggaat aacgctccat cacaataatg cccgaaaaaa tggcgatagt
     2221 tttgacttct cccttacaaa tttttgcaga tagagattcc aggccggtaa tgacttcgag
     2281 aaaggcacgc tcccaaatat caaaataata ctttttggga aaaataaata atccagcgaa
     2341 gattaccgcc atggctgcca aggtatataa agcattatta atagcaatat aaaaattgct
     2401 atctgcctcc acaccataaa tcagggaata ggacgccaaa cttagcatca atggaacaat
     2461 aacaagcatt tttttaaaat ggtgaagcac gaggaaataa agagccaagg tatagaaaaa
     2521 aacgaaaaaa ataaaaagca cgttgtatac agaaattatg ccgaataaga atatagagag
     2581 tatgcttcct aaaagggtat agagtaaaag catgtacttc tcttttaggg ttgttcccgc
     2641 cgcctccaca gtaaaacatg tgattggggc ataaaaataa tagaaatacg gattgtcaat
     2701 ggaagaaaaa aagtaaaata taatcatcag ttccaaaaca aatactgctt ttagtccatt
     2761 aatacgatgt tctttatacc tatcgtagga tgcgagctta tcttttagtt gagatataaa
     2821 cataagcact ggcgccagga tttaaaggat agtcaggatc gggatccaat atcttgattt
     2881 gtaatggaaa tcgctgagga agcaacagcc attcattttg atttgtaaca acctgaattt
     2941 gactctttgg agcagtgctt tgtcgttcag cagcccataa cgagttgaca acaaccccat
     3001 gaaatatttt gtcaaaataa tacatgcgta aaatgatata cgctttatca ccttgacgta
     3061 cattgcgtaa gtccgtttca ttaaaattag cctggatata ataatttgca gtatctataa
     3121 aagaaaacac cggtttatgg atctcgatag gggtatttgg agaaacatac atattatcta
     3181 tcactccatc accaggagct ctgacaatgg ttaaatcaag attaaccttg gcattatcca
     3241 tttttgcctt aaggctggcg acagtatgtc tttgttgttc aatcttggct tgcaaaaccg
     3301 ccacttcctg ttgcagagca tttcttttat tacttaaagt agaaacatca taaccaagat
     3361 tttttaattc aagaacagat actgcttcgg ccacgttctt atttttttta agaccaagag
     3421 caaattgagc tttttcaagc tcagcgtccg ttgctttaat caaagcaacc gttctttcaa
     3481 tctgcttttg aaacacgtct atttttttta atccttcctt gtaatcagcc attgcttgct
     3541 gataagccaa ctcataagga accctgtaaa cgctaaatat aggatcgttc ttctttactt
     3601 tttggccatt ctttacatag atttctgtaa taaaaccaga tacatcagca gctacagggg
     3661 taacattagt aacaacaaac gcattgtcag tgaaagggat caaataggaa aataaatgaa
     3721 aaaggccaat aataagagcc aagccaacga caataatggg aagattgatg taatttttgg
     3781 cctttttaaa aaacataggg atagaataac taaagaaaaa ccggatatgt ttagttacta
     3841 ttttatacca tgtggcaaac ttctctttca caatgcagcg cctctttgat ggtgactcta
     3901 taatacaaca tcagtttatc tctcaaagag agaaacccca tgacaaggcc ctcccttttt
     3961 cggcattaaa actaaaatac ctcttttttc cgttttcgaa acaatcgaga aagattgttg
     4021 ggtgactcat ataatcggct ttttccatcc tcaggtaaat aaccccattg agattcttct
     4081 gccagcacag ccttggcatc tgaataccca gcttccattc gcttcttaat gacaactcgg
     4141 ccaaagttgt aatccttcga agccgctttt actattcttg aactgtaagc caaatgaacc
     4201 aaggtacaat gatgggggct tccaatatct aacaaatgag cgctctcctt cttttgttca
     4261 tccgttaaaa tacggcttaa ttccatcatt gagtttcgca ttttttgtct ctgcaaataa
     4321 ttgtaaatag tacgttgagc atgggttcca tatgaaatgt cttttcttct ctcttcaatt
     4381 tcactcatgt ttttgggaat aaacgatcgc ccaccaaaac aatcaattag aaaacacaag
     4441 gtattttctg caggaatggc ctccagaata acttctaatg gggtgttaga gtgcacacca
     4501 ccatcccaat acaattctcc atcgatatta acggcaggaa agcccggagg taaagcagca
     4561 cttgccataa tgtgctccac ttctatacga taattgatat tattaaaata gattaaatgg
     4621 ccattgctga tttgaacagc accgagactt aaacgaatgg ggcaggaatt tagcaattca
     4681 aaatcaataa gatccagcaa tgtcttgcga agaggagtcg tatcataata gctcagagtc
     4741 gttggatcac cccataaagg aattgttccg gtgcaccatt ttggaaaaaa aaactcaggc
     4801 tggccaaata ataaagcaca agcggcactt aatttattat aaaaatctaa agtgatatca
     4861 gattcaccta aaaaatcgaa aaaattaccc ggggcaattt catcccaaaa tctttcgagt
     4921 ttttcaacac gcttttctgg aggatttcct acaataatcg ctgcttgtat ggcaccaata
     4981 gaggttgctg caatccagtc cggttcatac ccggcttcaa gaagcccttt aaccacacca
     5041 aattgatagg ctcccaaaga gccaccgcct tgcagcacat aagccacacg ggggaaacgt
     5101 ccttgatcct tgaggggtaa gttatcaaag cgattggcat aaggattatt accgtgcggt
     5161 ttaggctcca cttccaaact cataaaaact cctttttcac tttagttgat catcgcatcc
     5221 tggctgaaat gttgggaata cttgctgccg cttaataaca ctgttcaaaa tcagattgat
     5281 atatacattg ttattaaata acgtgagtta tggattacga ttatataaga agctgtcgat
     5341 atccagggtt cgttcgggct gaggagatgc gtaagcatcg tctcgaagcc ttggcaagaa
     5401 acattaaatt ccatacaagg ctttgagacg gcataaatgc ctcctcagcc cgaacgattc
     5461 aagataaagt atccataact cacgttaaat aagtttcgca gaaatcataa gtgtttaatc
     5521 attcacattg tactcataat cctttgagca accaagataa tatatgaaga tcattccgaa
     5581 tagttttaat tatccggcta aaatcaacac aataagcaac taaaaatgtc ctgtacgaaa
     5641 atcatgtatt gcctgcataa tttcttcttg agtattcatt acaaaaggac catagcgaac
     5701 aataggttca tgaagtttag ccgcagctat cagtaaacaa tgatttctat tttctttgtc
     5761 tttgccttct atttgtattc tttcaccgtt tgtcaggctg gctaaagtcc ctgcggtaat
     5821 ttgctgtcct cctgtattca cggtaccttc aatgacaaaa aggacagctt gataatcatc
     5881 aggaatcgtt tgttgaatag ttgcattggc agggagagtg atgtctagaa acaaaggtcg
     5941 ggtcgcaatt tcaacaatgg gtgaactcaa tccttgatca attcttcctg ctattacttt
     6001 gatggttgca cctgattcca tgacttcaat aggtagttga tccgatgatt tttcctgata
     6061 acgaggttct cgccattttt ctgcagcagg caaatttaac cacaactgca aaccatgcaa
     6121 ccggtccgtt gaagacgaag gcatttccga atgaataatc ccttttcctg cagtcatcca
     6181 ctgcacacca cctgcgttta ttactccttt gttaccttta ttgtcttcat gggtaatgct
     6241 tccctccatc agataggtaa ttgtttcaaa tccgcgatgc ggatgaggcg gaaacccggc
     6301 gagataatcc aatggatctg aactgtcaaa atagtcaaaa agcagtatag gttcaaagtg
     6361 attggtttta tcagctccta tatagcgacg taatttaact cctccaccat ctcgtattaa
     6421 tgttccctgg attaactgct ctacttccat tgctttcatc ttaattacca attaattact
     6481 gtcttatatt aagatagttc aaaatagtat ggtttcctaa ttgatttact attatttaaa
     6541 ctattggcac gttcagttat caaggatatg atgaaaatag actctaagga taggagcaga
     6601 atttatgaac acaacggaac atacagagct gggcaatggt ttgctcatgg ataaaatcga
     6661 aggcaatcca tatttacgaa tagatgagtt tggcgtgctc catctgcgac ttcaacgatt
     6721 cggtgaaaac aacctgcctg agccaatgga gctggagatg tccgctggcg aaattattgc
     6781 catggcgggt gactacttta cacaggctaa ttggactatg gatttggatc tgcccaaatg
     6841 cgagctattc aactccccgg cagaattggg aaaacacctc attcgcaaac ctatagatcc
     6901 taaagaagag aatgctttaa ttacagcata caataacctg gctgctcctg atgttactcg
     6961 aaaagaaatt gatcgcattt attccattaa taatgctaac tatgtgccct tttcgcctac
     7021 gttgaatttc tatgctcaac aactgatgta ttattttcgc gtcaaagact atggggaaat
     7081 gcttgttcgg aaccaaactc attttacgcc atggtctatt cgagtttata tattgggtca
     7141 cgctatcgca ttgagatatg cacgtttatc ctacgaatta aaacaattgg ctaccgacag
     7201 aaactatcaa tcagataacc ctgacttgag tactttaaaa atctcattac aaaataagaa
     7261 tgaaactctt tcaagcaata ccctgcttga tttggcgaat cgctatcacg cccaagctta
     7321 ttctatagag ctgtttactt ttcactacta ttctgatcat tttgctacag gacatatgtc
     7381 catgattggc gatttacgtg tcgtattaaa agagcgtttc ggagtatggg gcaatatcct
     7441 ggctaataat cttcatgatg aagtaaatcg tgttggagtt tatacagtga gaccctatga
     7501 tccaacccct aatacaacag aggctccgtc tcgagcacgt ggggatggga aatttgatac
     7561 ctgccttaat caatttaacc gtttagcttg cctaaacggt atgaccgcat cactaaagga
     7621 catcaatcaa gtattatcag gaggtgtaat accggagcaa aaaaaattcg gtggtttggt
     7681 gcatatgccc gacgttgatt ttaactcaag acaacatcaa ccattgctgg ttttaagtaa
     7741 aggaaaagta tattaccgca acaatctcag tcaaattcac ataatttctc cttctgaata
     7801 tgaggcactc agagaaaacc ctgaaaaaca tgggtataaa gagctaacca gtaaatgggc
     7861 tgcctttaaa ttagttacca aattacgtct ctttccttat ctttatgacg gctcggttat
     7921 gcctgtttct gatgaaaagc tcgccgaaat tatcgcagat gaaaaacgca gaaatccaca
     7981 acgagctccc atccccacac catcatgcct tccggaaagc gaaccaactg tattcgattg
     8041 gcgaacaaaa gcgagctggc gtaataataa agactctctg gatattttgg atgggttaaa
     8101 gaaacacagt attttggcag caaaacaaac ccattcggcc attcaagagg aagaagaggt
     8161 ccggttgaat ttgggagtct gattgtttcc tgataaacta gtggcctatt gttactattt
     8221 aacttttagt aacccatttg agctgtctta aagttagtaa gttggacata agcaaataca
     8281 tgcattttct tatgtccaat ttggttaaca ataataaccg taaacaattt tgactattca
     8341 agaggtgatc atgaagcgct taatacctac tttcatatac ctgctggttt tagcaattac
     8401 tcccttctct actcatgcag aagcgctttt gaaagacact tttggtcaca atattccttt
     8461 ttcatcactt aaaggaaaat gggtttttat caactattgg gcaagctggt gtgaaacctg
     8521 tgtcgatgaa atccctgaat ttaatcgctt ttacaaaaac cataaaaatg aggcagtagc
     8581 cctatacgca gtcaattacg atgctcttcc cttaccagag caaaaattac taattaaaaa
     8641 atttaatatt aactacccta atttgttaaa ggatccggct caggatttac aattaggaga
     8701 catcacagga gtgcctgtaa cttttgtatt taacccaaaa gggcaattaa ttaaaaaatt
     8761 atacggggga caaacggcaa aaacattaga aaaagtcatt gcagagaaca ggacctccta
     8821 agaagataaa taaaaattat atttatggtt aataagttaa agattttctt gtgaatttat
     8881 caagtctgga atcgattcta tcgccccgca gcctgacttg attattcatg aaagcaatcg
     8941 tcgtttttga tcagccgtta ttctaatttt cattaaccaa tcgccgtttt catctacaga
     9001 ctctttaagt acagcaccta attggtataa ttgggctcgt aattttgcct ggtcaggctt
     9061 aattacaata tcctcaatta aaatcatacc atgcaattga gttgctattg cctcttttag
     9121 aagatccaga ccagcgccgg ttgttgctga taaccacacc ttgcaagaat cctcagtata
     9181 atcaattttt ggttgccaac cttcctgcaa atcaatttta ttaaatacct gaattaccgg
     9241 gatattatta acttttaatt catctaacac cttttgtact tcaaaaaccg tctctcgcca
     9301 attcgggtca gaaatatcaa tgacatgcag taatagatca gcttgctggg tttcctctaa
     9361 agtagcgcgg aatgcttcta ccaagtgatg gggaagatct cgaataaatc ctactgtatc
     9421 tgctaaaatt gctgctgagg accctggcaa ttccaattta cgcatcgtcg gatccaaggt
     9481 tgcaaacaat tgatcagcca cataagtgtg ttcccctgtt aaaacattaa ataaagtgga
     9541 ttttccggca ttggtgtaac ccactagaga aacagtgggc atggacgcct tccgcctggc
     9601 ctgcctgttt tgatcgcggc tgcaacgtac tttttccaat cgcttattga tataacgaat
     9661 gcgctctctt aacaaacgcc ggtccgtttc caattgagtt tctcctgggc ctcttaaacc
     9721 aatccctcct ttttgccttt ccaaatgggt ccatccacga ataagccttg ttgataaatg
     9781 ttgcaattgg gctaactcaa cttgaagttt accctcaaaa gtacgagctc tctgagcaaa
     9841 aatatcaaga atcaagccac ttctatcgac aactcgacat ccaaataatc gctcgagatt
     9901 ccgctcttga gaaggcgata gctcatgatt gactaacact aaatccgcat cgagcatgtt
     9961 taccaaatga gctatttcct ccgccttacc tttccctaca taatatttgg catcgggagt
    10021 agctcgggca cctaatacac aatccaacac ctcagccttc gccgaaagcg ctaactcttc
    10081 aaactcagcc aatgccttat cagcatcaac cccggggaga gctaactgca ccaaaacagc
    10141 tcgctcaccg ccttgtggac gttcaaacac taccctaatc cccaaacaag aaattgatta
    10201 gtctgccaca gttccttctt cctcaccaga gctttcctca gcaggaattt tgaccattcg
    10261 agaaggaaca actgttgaaa tggcgtgttt ataaaccatt tgggttatgg agtttttaag
    10321 catcaccaca tattgatcaa aagaatcgat tatcccatgc aatttaatac cattgaccag
    10381 gaacactgaa acaggtacct tttccttgcg caattcattt aggaaagggt cttgtagtaa
    10441 atgattttta gacattgcct actccttgtt gttattgtaa tttttaaagg caaaaacggg
    10501 tatctaacta tttattcgac accctataat aaaactttag tatattttca tgacagcgtg
    10561 gtttgaattc gttaatatag tcaatcaaaa tatttttgca catctagaat caggctactg
    10621 atgagttaat aaggagattt taatgacgtt tacttgcgat gaattaaaag gattagagca
    10681 cccctacgaa gtacttggca atggtgacgc actggcggaa aaccgcgaag agctaaacaa
    10741 attaagcaat gacgaaaaat tagttttagc cagccgcctc gttttggagt gccccgttaa
    10801 tgaattaaaa gatttttctc atgccattga agcagcaaga atgccacaag atgatagcga
    10861 tacctttcat tcctttttat tccaggctta tcaggtaaaa aaaagaatca tttccttgct
    10921 cgatccgcgc aataaaaacc ctcattccat gatattggag aaggaatttg atggtgagct
    10981 atttaataat tttaataaat tggctattga tgtattaaat aacaatgaag tcgcaattgc
    11041 attgagactg gctgaaacaa ctcctgctca agatcgttca cgagtatctc aaaatatcaa
    11101 caatattttc ccacagtctc tgtttgccgc taaagtcggc cacgcttttg ccgttcgcag
    11161 agacatagag cgactattgc taggtgacag gcctgatcaa ttcttctcaa gtcgtgaatt
    11221 caagatcgat tcttgcatcg aatttgcaag tttattcaat gtaattaatg ataaagaatc
    11281 ttctattgcc ggaaaacttg cactgagaac tcctgctgaa aatcgcactg atgtagtaat
    11341 gaaaattaaa ggtttttgtg ccgaagatag tgaacttgcg ataaaagtcc aatctgccct
    11401 tgcattaaga agggatatag agcgcaatct attaggtgat aatccagaac aatttttcaa
    11461 tagccgagat tttagcgttg atttatgcct tgagtttgca attttattcc cggaattatt
    11521 aaaaggccat gaacaagcta tcggagaaaa attagctaag ctggatgcta aagtacgctc
    11581 agatatcagt agaaaactgg aaatgatcaa tggagcagct cacgagcaat caagcccttt
    11641 cagaataata gccagtgcca tggtgcctaa agaagagcct tgcctgacaa agcccactcc
    11701 cgttgtcttg ccatctactg aagaaattgt accaaagcaa gaatcaccaa ccagtttacg
    11761 caacaggaac gttttattta aagaacaaac agaagaacac aagaagaaaa actctcagat
    11821 ggaacccatt gtctcagatt ctgaagaaga agaagagaaa cagaattgct ggactagttt
    11881 ttgtgggtta tttggcaagt aaagcagcca tatcttttgc ccttacttgg ttaagggcaa
    11941 ctttacactt ttttacccat tctttacaca tcaggtagca ataattcatg ttaatatttc
    12001 tcataaattc attgacttat tgcaatctgc tcgagttaaa gacataaatt aacaattgaa
    12061 cgccgcttta aacttaataa atgattcgga cagaaaaact attgaacaag tttacttttt
    12121 tagctttaaa acaatagaag ccccttacct cacaaaaaat caaggaaaaa cgaaatgtgc
    12181 tgtcctaaat gaaaggaaag attcgcttct aatcgatagt cgcgagcttt caccactttc
    12241 agaatagctg ccattctgaa aattcattag taattagtaa cagccaaaat gagcagtgaa
    12301 ttgaaattaa tgctagtaca aagagagctt tttttgataa catagccaga tttttaatct
    12361 gggttgatga tttaacttaa gcaagagtaa gccattcgaa ttcgagataa aaattactta
    12421 acatcattta ttgggaactc taatgaaaga aaacaaacta acggtcgcac tggttcagga
    12481 acaatggtat gaaaatccta aagaacatca agataaactg gcttctggaa tattttcagc
    12541 tacccagcaa ggggcaaaac tcgtttgttt acaagagctt accttaagcc cttatttctg
    12601 tacacgtagt gacgtcgatc ccgcccctta tatggaggac atcgccactg gtcccactgc
    12661 tcaatttgtt agccaaatgg caaagtcgaa tcaaattcat attaccgcat cgctttttga
    12721 aaaagctggt tacaataccg cagttgcttt taatccccaa ggagaattga ttgctgttac
    12781 cagaaagcaa catatcccca gtggtgaaaa atatcatgaa aacttttact tcaagccggg
    12841 caactccaat tatcctgtcc ataccattgc tggtcactac ttgggattac caacatgcta
    12901 tgaccaatgg ttcccggaat tatcaaggat ttatggatta aaaggagctg aaattttggt
    12961 ctatcccaca gcgattggag gggaaccgac tgcgccagga tttgatagcc aacccatgtg
    13021 gcaaaaagtt atggtagcac aaggaattat gagtaacaca ttcattattg cggtcaaccg
    13081 gattgggtgt gaagatggtt taagttttta cggaagcagc tttatcagta cccctatggg
    13141 cgaaatttta gttcaggcac cgcgtaatga acctgccgta ttggttgctg aactggattt
    13201 tagccaacgt gaattgtggg ggcgattatt tccatttccc caacaacgag agcctgaaac
    13261 ttatcatgaa ttagtcaaat cccgttaatc ctctttggaa cgttagaatg aacaataata
    13321 ctctatcaga tgacaatctt tacaacatag aaaattgggg cgagggatac ttccgaatta
    13381 actccaaggg aaatattgaa atcagtaaaa aacccgggaa accgggtgtt gaactgcaaa
    13441 ctatagttga tgcagctgat cgtgctggct tgcatttgcc tttattgatc cgttgtagtg
    13501 atattcttca tgatagagtc catagaatat atcaggcgtt cacccaagct cgagaagaca
    13561 cttgttatgc cggtaattat cagctggttt atccaattaa agtaaaccag gaacaaagtg
    13621 tggtacggga attgttaaaa tcgcctgata atcagatagg cctggaggca ggcagcaaac
    13681 ctgaattgat ggcggttata ggtatgctgg gtgaccatca gagtactata gtttgcaacg
    13741 gctataaaga cacaagttat attcgtacag cgcttattgc tcaacaaatg ggtcataagg
    13801 tttttattgt tattgaaaaa aaatctgagt tggacattat tttaaaggag tctgcacgcc
    13861 ttcaaatcaa accaacatta ggtgtgcgaa ttcggctggt cagcaagagt gctggcaagt
    13921 gggaaaatac cggcggggtt aaatcaaagt ttggactaaa tgccaaacag gtacttgaac
    13981 tgattttaca attaaaagcc aatgatatgc tggattgttt gcagctaatg cactgccatt
    14041 taggttcaca aattgccaac atccatgaca tacgccattg catgcaggaa gtggcgagat
    14101 actatattga gctacgtcaa ctcaatgctc cgataggtac catcgatgtg ggtggaggtc
    14161 tgagtgtcga ttatgagggg actcgatcca ataaaggatg ctcaatgaac tacagtcttg
    14221 ttgagtatgc tacgcacatc atgttagctt taaaacactt atgtcaagaa gctgagatat
    14281 cagagcctga tcttatctcc gaatcaggac gagcattaac agcccatcat gctgtattaa
    14341 tttctaatat tacggatgta gagattatca ataaagcgcc cgttcttcct gaaatatcgg
    14401 aagatgattc gcatgtcatt cgagatattt acgatactta tcaagccatt acagatagct
    14461 cgcccactga aatatataat tacgcagctc attcgcttaa tgaagcgcat tcccttttca
    14521 agcatggggt aattagcctg caggaaaagg ccaaggtaga agctttttat actaatattt
    14581 gtatggaaat aaaagataaa ctggatgaag aaaatccaag tgaaaaagct ttgctaatga
    14641 gaatcaatga atgcatggct gccaagatat tttgtaatat ctcttttttc caatctattc
    14701 ctgatgcttg ggctattggt cagattttcc cggtagcccc tatatctcaa cttaatagaa
    14761 cacttagtat gcatagtatc ctacaggatt taacctgcga ttcagatggt acaattaagc
    14821 aataccctgg aagttcctgc gttacaacca cattgatgct cccttcatac gatatcaata
    14881 acccttataa catcggattt ttcctggttg gtgcctatca ggaaatattg ggtaatctgc
    14941 ataacttgtt tggagatacc aattctctag atgtaaagct ttctgatgat ggacaatttg
    15001 agctggatga tttagtcagc ggcgacaccg taacaaacgt gttgaattta gcccattttg
    15061 ataccaaaaa gttaattcaa tcttatgaaa agcaattaat taaatctgaa cttcctaagg
    15121 aaaaggcact ctcttactta aacgaattaa gaagtatttt ttctcaatta acttaccttg
    15181 atggaaatat agcacagtga atatgaatac agcacccaaa caatatggtt tttatatgcc
    15241 tgctgaatgg tatcctcatg aacgatgctg gatggcatgg ccctgtcacc atgaaacctg
    15301 gtccaaaata gggctagaca aggctaaaat ggcctatgca cgtgttgcaa aagctatagc
    15361 tcaatttgag cccgttactt tattagtaaa cccaggagat gaagattccg cagagaattt
    15421 atgcaaaggc cataatattg aaattatctc tttgccaatt aacgattcgt ggacccgcga
    15481 tacgggggct acctttttaa ttaataatga aaggcagctt gccggagttg actggataca
    15541 taatgcttgg ggtggaaatt acgctgactg ttctttggat aatttaatag cttctcatct
    15601 aataaaatac actgaagctc aatactttca tgcgccattg gtcatggaag gtggttcctt
    15661 tcatgtagat ggtgagggga ctattttgac aagcaaagag tgtttgttaa attccaatcg
    15721 aaatccacat ttatctcaac aggaaatcga acagtacctt attaattatc tgggtgctga
    15781 gcgtattatt tggctgaaca tgggattgat aggtgatgaa acagatggtc atgttgacga
    15841 aatagcaaca tttattgctc ctggaaaggt gttatgcctt attaccaagg ataaggaaga
    15901 tcctaactac catcgcttgc aggaaaactt tgaaattctc aaatcatcta aggatgccag
    15961 aggacgaaca tttgaggtct atactgttga gcaaccacct gcaacttacc ttaacggaga
    16021 aaggctcact ctctcgtaca tcaattttta catggctaat caaggtattg tgatgcctgc
    16081 ttttggatat gaatcatttg acagattagc ctatcagtta ttcgtacaaa tttttccagg
    16141 atatcaaatt acacaaattg atgcactcga tgtattttct ggtggtggtg gtatccattg
    16201 tattacacaa caacaaccta aatctcataa attagtggag taatgacgaa gacaatgcca
    16261 cttcttaatt gagaaatttc ttacatatta ctgctcatta tactcgatga catatactgc
    16321 caattggtta tactaaaaac atacagggat gacatggata tgtctgacag gaagtcaact
    16381 aagggaccag tacaaaggat gtacttcgct gcaaggacgc agcacttatt ctccaattta
    16441 ccagtatacc tctttgattt ttttctttta agcaatcaat caaaaggctt tttttgaaca
    16501 catcatcata gtaggaaaat aagttaagtc attgagtgga ccagctataa gctagagagg
    16561 cgtttgccac gagcgaggca aacatcactc cagttcaaca atactatttg tttaaatatc
    16621 aatgttttca gcgtctaaag cattcgtttc aataaaatct cttcttggtt ctacattgtc
    16681 gcccattaag gtggtaaaga tttcatcggc agctacggca tcttcaatgc tcacttgcaa
    16741 cattcgtcta acctcgggat ccatggttgt ttcccataac tgctcagggt tcatttctcc
    16801 aagaccttta tagcgctgaa ttgtttgtcc tttttttgct tcatccatta gccaattaag
    16861 tacttgctca aaattttcca caggcaagga tttatctcca cgttgaatat aggcgccttc
    16921 ctctattaaa ttcgacaatt tatgtcctaa atcaaccatt tctttgtaat ctttagacag
    16981 gaaaaattca acttgcatcg gaatatcatt ttccaagcca tgctggtaca cagtaatttt
    17041 tggggtgtat tgattacctt ctgtaagttt ttgggtatct actttgtatt gttctgtttt
    17101 actgtctact tgttgtaagc ttaggtgtaa ttgtctgcac cagttttcaa tttttggcag
    17161 attgttaaaa tcttcttggt gcagcattgg catataagct actcttttta gtatatcgct
    17221 ggggtatctt ctcttgtacc gctttataat cttctcaaca cgtctgaaat gttgaaccaa
    17281 ttcttccagc gatttggcag atatgggcgg aacctctttg gcgggataaa atgtcgcccc
    17341 atccaacgcg cactgagtta gataatcaaa taacgcttca tcatctttaa catattgctc
    17401 ctgctttcca cgctttactt tatacaaagg tggttgtgca atataaatat atcctcgctc
    17461 taatagctct ctggtttgtc tatagaaaaa ggtaagaagt aaagttctta tgtgggaccc
    17521 atccacatca gcatctgtca taatgatgat tctatgataa cgtactttat ctggatcata
    17581 ttcctcggga ccaattccac agcctaatgc agtaatcaaa gtggctactt cttgagaaga
    17641 aagcatcttg tcaaaccgtg ctttttcaac attaagaatt ttgcctttaa gaggtaaaat
    17701 agcctggaat tttctatctc gcgcttgttt tgctgagcct ccagcagagt caccctcaac
    17761 taaatacaac tcggaaagcg atgggtcttt ttcttggcaa tcagctaatt tacctggtaa
    17821 accggcaata tccaaagccc ctttacgtcg agtcatttca cgagcacgcc gcgctgcctc
    17881 tctagctctt gctgcatcaa taatttttgc tacgatggat tttgctgcag tgggattttc
    17941 taaaagaaaa tcgttaaatt tttcagcaac acaggattct actgctggtt ttacttcaga
    18001 tgaaaccagt ttatctttcg tttgcgatga aaacttggga tcagggactt ttactgaaag
    18061 aacagctgtt aaaccttcgc gagcatcatc acctgttgga gatacttttg cctttttggc
    18121 atacccttcg ttttctatgt aattatttaa agtgcgagtt aatgctgctc taaagccggc
    18181 catatgtgtt ccaccatccc tttgcgggat gttgttagta aagcaaaata tggtttcttg
    18241 atagccatca ttccattgca tggccagttc aatgacgata ccgtcttttt ccgatgtcat
    18301 tgagaaaaca acggggtgga ctggtgtttt atttttattt aagtgctcaa caaaggcttt
    18361 gatgccgcct tcgtagtgaa acgtgtcttt tttctgtgtt ctttcatcaa ataagtgaat
    18421 acaaacgcct gaattcaggt atgacaattc acgtaatctt ttagctagaa tgtcgtaatg
    18481 gaactcaata ttagaaaagg tttcagcgct tggtttaaac catatttgtg tgccagtaga
    18541 gttggcttct ccagtttcag caataggaga ttcaggaaca ccatgcctgt agtgttgctc
    18601 atacactttt ccatgtctac gcactagtaa atgcaactct tctgaaaggg cattcaccac
    18661 agatacccct actccatgta gaccgcctga taccttgtaa gagttatcat caaatttacc
    18721 tccggcatgc aggactgtca taatgacttc agcagctgat tttccttctt ccttgtgaat
    18781 atcgacagga ataccacgac catcatcctt aacggtaatt gattcatcgc tatgaatggt
    18841 aacaaaaatt tccttgcaat aacctgccag ggattcatct attgaattat ctacaacctc
    18901 aaaaaccata tgatgcaaac cggttccatc atctgtatcc ccaatgtaca tgcccggtct
    18961 ttttcttact gcgtctaaac cttttaggac tttaatatta tttgaatcat aactggcgtc
    19021 tacgctcatc agctgccctc ttgccgtatt atgctattaa caatgagtct attatatcat
    19081 cttttgtatc tagtttatat cttaagtaga gaactttacg cttgtgcatt ttgaattatt
    19141 gacgttgatt ttttttgaat atcattttgt ttatttgatt ttcataccat gacatttgat
    19201 gagccatttg tagaatatca tatgtttacc tttagaattt tatattgcat ttgcattaag
    19261 gtgatattgt ttaataatga gaacttttaa ttccattaat ttgtgttcca cgtgaaacat
    19321 tctatattat aaatttttaa ttgcgccaca gtttacttca aacaaacctg agtcaattgg
    19381 caggatatcg taattattgt tgttaattaa attcgttata acaaactgcc ctttttgctg
    19441 tgtcagatac ttaattaaat ttcgctggtg atagtcatct aactcagcag ccaaatcatc
    19501 aataagatat aagcaatctt tatctaatag ctgaccttga gccagtttaa gcgcgattag
    19561 aattattttt tgttgtccac gagagagtgt atgctttact ctgtattgtt cagtgctaat
    19621 tatcaaatca gcttgatggg gtccatattg cgtatatagt ttttttctat cactatcaaa
    19681 tgatttctga agcagttcct ccatgttctg tcctgcattt tttctatccc accctttgta
    19741 atactcaact gtgcaagata tattggttag atcgcttaaa acttggtaga atttaggttg
    19801 ccattgcaaa aaatagtcat ttctagcttt atccagttga tttgccaatt gacttagctg
    19861 ttggtcccaa ggaataaaat gatcataagt agctctggat tttaataagg catttcgttg
    19921 gctaagtatg cgcttgtaat ccttccaaat tttcaaataa tcatgtttca cgtgaaacaa
    19981 cccccaatca agcaagcttc tccttaccga ggggccggca tcaataattt gaaaaatatc
    20041 tgagtaaata acctggcaag gaagcgcata ggctagttga cttgtagtac agcaaaactg
    20101 gttattcaat ttaatttgtg tgccatctgt tatagatttc tgaacactaa tagtgctttc
    20161 gtcgtaagca tgggcaaaaa cattaagttg gttttgacca tacgaaataa ttggagttat
    20221 ctcacgagac cgaaaagaat gggcgcagct caacatataa agagcctcta gtaacgaggt
    20281 ttttccaccg ccattaggtc ctgtaataca attaaaattg ggatttagga ttaaactagt
    20341 cgaagcgata ttccgaaaat tatgaattct aacttcagat aaaatcatat tttcataggc
    20401 ataataatgt attgataatt atcgttgact aatgactcaa ttaaaatact gctttcagta
    20461 ttagacattg ataacctgat ttgaccttca gaaaaatgag ttaaaacatc cagcaaatag
    20521 gttgcattaa gtccgatttt gagttcatca ccttgtgttt cggcactcaa tgtttcaatt
    20581 gcctcttctt gctcattatt attggcaatt aaagttaact gaccttgttg taaatgcaac
    20641 aagacagcct ttgacttttc atgagctaaa atgacaatac gagataatgc acgcttgagc
    20701 gaagcgcaat caataacaat acatttatcc tgctctcttg gtatagcttt agtgtaaggt
    20761 ggaaagcgtg cttcaattaa tttagacaaa aatacaaatt gattggtaat caacttaata
    20821 tgagattttc ccgctgccaa caaaacttgt tcgtcattaa tacaattcaa taatcttaat
    20881 aattcttgca cccctttttt gggtataagc aattttgttt gagcaatatt attacaggaa
    20941 tatcgcgata tagccattct gtgcccatca gtagcaaccg ctgaaataat attactgtca
    21001 aactccagca ataatccatt caaaaatata cgaacatcct gctgagacat agcaaaatga
    21061 gtagattgca ataactgcaa caaaaccaaa cgcgggatag ttaactcgac atcattacat
    21121 tcatcttcac tactaggata attctcagca ggcaaagttg ccagtttaaa tgtacttcgg
    21181 ttttgtttta tcgtcactaa agctttatca aaaacgatag atggcgaagc gttttcatct
    21241 aatgacctga taatatcaat gaatttcttg gctggtactg taatagaacc actagtattc
    21301 ttggaatcac atgccacctg tgcggacatt tcaatttcca aatctgtagc cgtaatatac
    21361 aaaagtccat ccgacagttt taataaaaaa tgggacaaaa tagccaaaga ctgcttctta
    21421 tctacggctc cggaaacagt aagcaatgga gtaagcagat cttctttttt aatggttagc
    21481 tcaaacaaga tagactccgt atcaagaaga taatattcgc attaaatttt tataatcttc
    21541 agcaaaatcg ctatcatcct ggattaattc tttaaccttc cgacaagcat gtatgactgt
    21601 cgtatgatcc ttaccaccga aatgatcacc gatttcaggt aaactatgat tggtcaattc
    21661 tttactcaaa gccattgcca tttgccttgg tcttgcaata gaccggcttc tgcgttttga
    21721 aagtaaatca gcgaccttta ctttataata ttcagctacc gttttttgga tattttctat
    21781 agtaactaac ttatcttgca aagcaagaag atcacgcaat gcttcatgta caaattcaat
    21841 agttatcggc ttaccagtaa agtgagcatt ggctataact cgacgcaagg caccttcgag
    21901 ttctctgaca ttcgaacgta tacgtttggc tataaaaaag gctacttcat aaggcaattc
    21961 aatattagat tgctcagctt tactaattaa tattgccacg cgcgtttcta actctggagg
    22021 ctccactgct acagtcaatc cccaaccaaa tcgagatttt aaacgctctt ccatcccttc
    22081 tatttcctta ggatatcgat cactggtcaa aatgatttgt tgttgtcctt ccagtaaggc
    22141 attaaaagta tgaaaaaatt cctcttgtga cctgtccttc cccgcaaaaa actgaatatc
    22201 atcaataagt aaagcattta aagaacgata aaaacgttta aattcattaa tagaatttgt
    22261 ttgcaatgct ttaaccatgt ctgcaacaaa acgttctgaa tgcaaataca atactttagc
    22321 ctcaggatta ttctttaaaa tactattacc aatggcatgc atgagatgtg ttttacctaa
    22381 gcccaccccc ccataaataa ataagggatt ataagcatcc ccagggcgct cagctacctg
    22441 catggacgca gctctcgcca gttgatttga atttccttcg acaaagctgt caaaaacaaa
    22501 ttttttattt aagtgactgc ttttatagtc aactgctttt ttaggagcgg attttagagg
    22561 tgctgtacta gatgaagtaa caattgtttc agcaggcgta tcaacagctt caacaggctt
    22621 actacctact tcaatagaaa tagctttgat gtcatcgccg gagaactgtt tgatcaactc
    22681 ttcaatacga gaaaaaaaat gttttctaac ccaatcaaca acgaaacgat taggcgctaa
    22741 taagattaaa cgctgctcgt ccatgtaagc ttgtaaaggg cgcaaccaag tattaaattg
    22801 ctgagcagaa aattcatctt gcaacaaacc taaacatttt tgccaagcag tagttgacac
    22861 aaaataactc ctaatttaaa caagtgtggt aaaagaa
//
LOCUS       DASLOE010000034        22540 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.034, whole genome shotgun sequence.
ACCESSION   DASLOE010000034 DASLOE010000000
VERSION     DASLOE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22540
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.034"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..2162)
                     /locus_tag="U6309_001064"
     CDS             complement(<1..2162)
                     /locus_tag="U6309_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEN5504517.1"
                     /translation="MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGI
                     VINKVCDQQTGHSLKKITSIIDEKPLVPEDLLELCLWVGSYYQSPLSEVIPLVIPKKY
                     RLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
                     NGTQIAALLACQAINLSQQVTLPTKIPEKLSPPLTLNPEQAVAVDSIFEHIHHYQCFL
                     LQGVTGSGKTEVYLHVIAKILEQGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSN
                     LNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEHDSSLKQMEGVRYSA
                     RDTALMRAHLANIPIILGTATPSLETIHNYKQKKYNLLRLTHKAASTTPLHYQLIDLR
                     SQMVQHGLALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLT
                     LHKQAGQMICHHCGLTQRIPLFCNNCQSKELIPVGSGTQRIYEFLSHQFPDTNVLRID
                     RDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGFYNQDFR
                     AIEHLGQLLTQVSGRAGRAEHPGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQE
                     AEMPPFQFLAVIRAQGKTVNKVEQFLKATKDQIQAHPLTVLGPAPAPLPRKANQHRMQ
                     LLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVDP"
     gene            complement(2163..2657)
                     /locus_tag="U6309_001065"
     CDS             complement(2163..2657)
                     /locus_tag="U6309_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEN5504518.1"
                     /translation="MKKYLTTVELQKESMKFSAGHTTIFSATEREPLHGHMYSVYLAL
                     TTWVEENGMTFDYRYYKERIHKLCRYLNQTFLMPQFSPFLEYSEDEEYYYFIFNKKKI
                     PFLKEDITLMPLTNITVEELSRWFVNELIKEKEELDNHRIEKVVVKVFSAPGQSASHE
                     WLRS"
     gene            complement(2660..3772)
                     /locus_tag="U6309_001066"
     CDS             complement(2660..3772)
                     /locus_tag="U6309_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEN5504519.1"
                     /translation="MNLKQTLINQLKLQKEIPFIDFMQQALYAPYEGYYTSGLQKLGK
                     HGDFITAPELTSLYGKTLANQFQQILPLLDSPVLFEFGAGTGKLCIDTLTHLEQLQCL
                     PDSYFILEVSANLRHRQQELIQQKIPQLAGLIHWLDKWPEAPFNGVIIANEVLDAMPV
                     YRFMQSETEILESYVALDEHDQLAEIFKPVQNQRLLAYIKNHLPPLDYPYLTEANLFL
                     DDWLLNCSHMLKKGALFIIDYGFPRHEYYHPDRNQGTLMCHYQHYSHPNPLLNPGEQD
                     ITSHVDFTHVAEAGHQAGFHVAGYTNQASFLLANGLLSLIHTLDDEQELFAAKQAIKQ
                     LTQPSEMGELFKVIALTKDLEIDLNGFLINDKRVNL"
     gene            3949..4704
                     /locus_tag="U6309_001067"
     CDS             3949..4704
                     /locus_tag="U6309_001067"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEN5504520.1"
                     /translation="MNQTNTQEAKVALVTGGAKRIGAAIVKKLHLSGYQVAIHCRSAL
                     HEADILAKHLNQVRMNSAIVLQKELTEPGAAEEIISKVKHWAGRLDLLVNNASVFKRT
                     DCHFLNEADWQYLFAVNVQAPFLLSIAACPLLAPCKGSIINITDIHADKPLKGYSVYC
                     QTKAALEMQTRSLARELAPEIRVNAIAPGAIAWPENDNSLTAEAQQKIIEKTPLKSHG
                     KPDFIAQAVLALAENSFITGQTLKVDGGRSLTS"
     gene            4761..5120
                     /locus_tag="U6309_001068"
     CDS             4761..5120
                     /locus_tag="U6309_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504521.1"
                     /translation="MSKWILLSGSLFFFLFSLGVHSNSFDKEQLVQRCQILHEELKEL
                     ESHQYNGVCRHKLALAANKMFSAKIRIVYENYKGAKQDLSVSMNNMKFAEDISCVFKS
                     EITKARMEAREIQRELN"
     gene            complement(5179..6165)
                     /locus_tag="U6309_001069"
     CDS             complement(5179..6165)
                     /locus_tag="U6309_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEN5504522.1"
                     /translation="MHTFINSPLQIGSLKLPHRLIQGPLAGYSCAPFRDLFNIYTPPA
                     YCVSEMCSAIDILHKHSSQSRYIYRAPTEKFLAYQISGNEPHVLAEAAAKLQSLGADL
                     IDINCGCPKSKIRKKGAGSALLEDPQKLIAIISRVRAAISIPLTIKIRIQNNEKDLTL
                     AKQIEDAGAHALIVHGRRWTEDYDIPCNWQQIANIKNAVNIPVIVNGDINNIFSLRKA
                     LEISACDGFMISRAGTGKPWLYRDLLNLETNDVAFDEKLKLFMLHLEGLSYLENEHKA
                     VLQSKSLVRYYFKKQLNEQQLQQFYQLDSLMEIKDFLAEATHRVSPILVAAS"
     gene            6247..6690
                     /locus_tag="U6309_001070"
     CDS             6247..6690
                     /locus_tag="U6309_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504523.1"
                     /translation="MDRRYLSPLELLGIATQHAYTADYMLQQIANGMYRGGETIAVFS
                     PITSLMYVAFQLTFKAYCLHDHRPIKEYKNLMELVELNSHLGLSSNDIFLLKTLSRQQ
                     VFNKGVDYDLWENQQQLHVFCEEIISLYERVQSMMPLELQSDYQE"
     gene            6867..7862
                     /locus_tag="U6309_001071"
     CDS             6867..7862
                     /locus_tag="U6309_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEN5504524.1"
                     /translation="MEQLDDKVADNVQQKILQLSRHLNSRILGQKDLISRLLIALLAD
                     GHLLVEGAPGLAKTRAVKELSDVVEGDFHRIQFTPDLLPGDLTGTDVYHPQNGSFVFQ
                     PGPIFHHLILADEINRAPAKVQSALLEAMAERQVTIGGKTYPLPELFLVMATQNPIEQ
                     EGTYPLPEAQLDRFLMYVKISYPDAKVEHDILTLARKEALGNATENQIKVTKLSQKTL
                     FEARKQVLKVHTSEALENYLVQLVVATRNPGVYSDELGRWLRFGASPRATIALDRCSK
                     AHAWLAGRDYVTPDDIHVIAHDVLRHRILLSFEAEAEGISSDDFIDSLLRLVAIP"
     gene            7872..8783
                     /locus_tag="U6309_001072"
     CDS             7872..8783
                     /locus_tag="U6309_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEN5504525.1"
                     /translation="MTDGVVAELSELIDLRRYVHSVHYKPQGRALRSGNHISKFRGRG
                     MDFAEVRNYQAGDEIRHMEWRVTARTGRPHVKIYQEERERPVVLLVDFNPSMIFGTKI
                     AFKSVVAARLASLLAWTVIKQGDRVGGFFFSATEHSEFIPRGRDSGVLPLLASLSRYT
                     TQTEVQREEKPRMLSDALMRLRRVIRPGSILVLISDFYSMDVECEKHLNRLRGHNDIL
                     AYHVCDRIELAPPKPQQYAISDGKQEIILDTGLETVSTAYQLYCQQRIEQLNDQFKRL
                     QIQYVQVTADTNLTPLVRLTFPRRSRG"
     gene            8776..9261
                     /locus_tag="U6309_001073"
     CDS             8776..9261
                     /locus_tag="U6309_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HEN5504526.1"
                     /translation="MAKTEPLAQLRDIHLPEPVGWWPLAPGWYGLMVLIVVLVAGVAY
                     FIYKRHVNALPKKQALTLLKIHKEQYEKDKNTQLASAHISELLKRVALVYYPRAEVAS
                     MHGEAWVEFLNQTGKGIDFTPVKSMLLDSPYKTSDALNLNPLFTRAEKWIKQRGKPCS
                     N"
     gene            9225..10283
                     /locus_tag="U6309_001074"
     CDS             9225..10283
                     /locus_tag="U6309_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN5504527.1"
                     /translation="MDQAKGEAVFELATPWALSLIPVPLFLWYLLPRAKVKLPAALKV
                     PFFEAMAGIVEQENRLISAKTLLLIPVLVWVLLVIALSGPRWVGEPKPVAREGYNIMM
                     VLDLSGSMEITDMLLHGRPVSRLLVVKRAAEQFVEDRVGDRIGLILFGTRAYLQTPLT
                     YDRHSVLMRIDDATAGLAGKTTSIGDAVGLAVKRLQDVPSKGRVIILLTDGANNSGVL
                     APLKAAELAKQDGIKIYTIGLGSEADPRALTGDFFAPTLSAELDEKTLEKMAKMTGGR
                     YFRATDPESLQSIYQTINQLETVKQEQATVRPQKEYYPWFVAFALLLCFYWLISKADL
                     FVYLRSFLKVERSLSHDS"
     gene            10273..11205
                     /locus_tag="U6309_001075"
     CDS             10273..11205
                     /locus_tag="U6309_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN5504528.1"
                     /translation="MIAEFHFLRPWCLLMIVPLFALVVIYWRQKPKLHAWEAVCDPHL
                     LEHLLHSKGQSKRALSLSFLFFSILFMILSVSGPTWYKLPQSTYKPIQPRVLLLDMSD
                     KMMLGDLSPNRLSRAKFKLHDLFTRKDIGQFGLIVFTGEPFVVSPLTDDGQTISSLLS
                     ALTPDIMPVAGYNLDSALDEASQLIKQAGYDHGEILVLTAETPSSSAIEKAKKLAESG
                     IFSSIMPIRADENLNPLFQRFANAGDGYLLVYSPDSSDLEKWLNASQGKEKFALEKNE
                     DIPLWRDEGRWFLIPALLLLLPVFQRGWMQRVTI"
     gene            11202..12128
                     /locus_tag="U6309_001076"
     CDS             11202..12128
                     /locus_tag="U6309_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN5504529.1"
                     /translation="MMWRVLTIVAAALISNQLKAFSWSDLWFTPNQQAQHLMEKGQFA
                     KAKDLFERNDWAASAAYRAGDYEQAAKLFNELKTEQGYYNQGNALAHLGKYEEAIRAY
                     DKALAFNPNNQDALYNRKLISELLKKDKEKKKSQNQQDKDQQNQDQQNQDKQNKDQQN
                     QDQQNQDKQNKDQRNQDQQNQDKQNKDQQNQDKQNQDKQNKNQQNQDQQNQDKQNKDQ
                     QNQDQQNQDKQNKDQQNQDQQNQDKQNKAQQNKVQNKKNDKQEGEIQSEANQEKQLAK
                     EQWLRLIPDDPGGLMREKFLRDHLRRERGWYQ"
     gene            12125..13774
                     /locus_tag="U6309_001077"
     CDS             12125..13774
                     /locus_tag="U6309_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEN5504530.1"
                     /translation="MKKLMLWGLLSLFSSLSFAEIKVELEPSQVTMGETFKLSITQED
                     QQGGGVPDLTELQKDFVILGTERHVNYSIINGQAQSQSQWIVLLRALKPGIFTIPPIK
                     VGLEQSLPITINVQGSANTQALPDEAIQKQDVFLTAEVNEKNPYVNQQIIYTVKLYNS
                     KRLLDADYQGPKVDDALLIPLGDAKRYHAVHNNINYVVEEQTYAIYPQKSGTLKIISP
                     IFNALVYDYNPQRTKATDKAINLTIKPIPKKYQGKLWLPAKQVKLSEQYENSNQTLSQ
                     GSTLTRTVTLEGIGIPAQLLPTLTFQETDFFSVYPEKGAERNQVKQGELIGSVEIKVT
                     YLFNKAGKTTIPELRLTWFNTETGKEEIAVLPPRSIDIIPSTAVKANTANTQVTGKEM
                     SASSQLAPDSASVANESGGWAVKLAIFFGLAWLITLSLWIWQKRPRESSSKKEYKKAL
                     DALNKACSQCNPKKARDAVLKWASLQWPDAPMLNLTDLAQLVRDAHLRKQLHLLSQVL
                     YRSQEKTLWRGDELLRAVQAVKRNESVKKRKNSNLPPINPF"
     gene            14011..15393
                     /locus_tag="U6309_001078"
     CDS             14011..15393
                     /locus_tag="U6309_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEN5504531.1"
                     /translation="MHVNEYIHYDVQELAELIKNHQVGAEEVLRCAQERLYQVNPSLN
                     AVVTDCFEYAHQCLAKMKGNEPYYGVPLLVKDLGHAIEGIRLTEGSRFFSNNLAETNS
                     DLINKLIALGFVPFAKTNTSELGLSCVTDSNLFGPCRNPFDTMRTAGGSSGGSAAAIM
                     AGIAPVATASDGGGSIRIPAACCGLFGFKPTPGLVPSGPLVDEAWSGMATSFILTRSM
                     SDAIALFKNVINPMRLNRLSRKKKKLLITELTGAFAEVPIARECQEAVEKIKEILKNS
                     GHRLNEVHLPLDLQVISSCAHTLIAASTYAKIKTQEQISGRKPMIHELEPITWEFYYR
                     GKMVSAYEFIIAKNKLYQSLRPLHQLLEKTDIVLTPALAQLPLLIGHLRTDEEFNKYL
                     QKKKEFSPFTSLFNQGGLPAMTVPVMFHHHLPVSVQMGSAKGNDLLLLELAHELQSVL
                     PKNITPASIP"
     gene            15675..17132
                     /locus_tag="U6309_001079"
     CDS             15675..17132
                     /locus_tag="U6309_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN5504532.1"
                     /translation="MKTLPSKVIFIGAGPSGLFAARQLKEIAKLQNKTVECIFLEREP
                     VVGGKCHTYSDAAHPELKTEWGAALVAPNYGVVLDALAEHGIEFEKVMPTASETIEIK
                     QLFEKASSLEKISMEMKLLRELWAFNSDYDVYKKAKQNKKALPDQLLNPFSEYCKLNN
                     MSYLPVLLKPFVPGFGYGDLNFCPAYCVLEYMGKVTMPDILLADQLIGRPSLLAIHGG
                     FQLLMERIAEQFDVRTSASVSRIKREKNKVTVTYVHNGIEQTETADTLVLATSPKNWK
                     SLGLDLTEVENNCIESLDYYRYPVAVYKIKGLPPQQYFFTKALEERGFGHLALITTRD
                     NRSNPEDGRLCTVYVNLSPNNNEFKFDHEALRKELESIPDVTEVTVVKDKIWEDYMST
                     LPWDLRLQLDKEQQRTNTMYLGSYALGSFEDVACVANKATDAMTEWYAPTVRYEEDFS
                     WKNMNRAWQFFTSPTKPPLASLGEPRVSDEHSNRP"
     gene            17372..17587
                     /locus_tag="U6309_001080"
     CDS             17372..17587
                     /locus_tag="U6309_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN5504533.1"
                     /translation="MSVKETGHVKWFNDDKGYGFISRDNGQDDVFVHFRSIVSNAGRK
                     TLLEGQRVEFLVTKGAKGLQAEEVTAI"
     gene            complement(17687..18991)
                     /locus_tag="U6309_001081"
     CDS             complement(17687..18991)
                     /locus_tag="U6309_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5504534.1"
                     /translation="MTQQDPVVIKSKGDQALEKGDLPLALTLYDEALAINPQFSGAYY
                     QKGTVLLRMGKTIQAAAMYWQAYLFSEFKTDIGLMTAKTLAHANYSLSACKLFESFKI
                     EEMDGESLAYYLYALRQQGRVQEAYSLFPYVNQFNIPKTSWARAIILLDLNKVEEARF
                     LLEPLEKTDKDGHITILLHAVYLALNDKKAVKSLLDRAIQRIPNNDYFCCQRIAIDIL
                     NGLNKTPIETYRAFKRFDLIDAADYLHKHADKHLRHSGTTYQTFDLLAPQVLSEGLIL
                     EFGVRFGYSISHIAKLFPKRKIFGFDSFQGLPEDWHHEKAGSYSTYGKIPSVASNVTL
                     IAGWFNETLPDFKKNNREPIAFMNIDCDLYSSTKLVFNELNPQIAPGTIIVFDEYIGN
                     INWRQDEFKAFQEWVKENKVEYRYLTASFYTKQVSVQILKRK"
     gene            19154..20134
                     /locus_tag="U6309_001082"
     CDS             19154..20134
                     /locus_tag="U6309_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEN5504535.1"
                     /translation="MIKPITLLLLFCSFTVNASILVNGTFEATKSCPAYLSKNRKSNP
                     DNLMVEPNQKYQLKEINKTKPDWLRIQFSDHQPNLRWVSVDCGVIEYTERPKASCDLS
                     PGMADSYVLALSSQPGFCETYGYEIGKPECLKLTKTVYQANHLILHGLWPNQEACGHN
                     YGYCGISPRTNHCDYSPLDLSSDVAQNLKKLMPSYNYGSCLERHEWNKHGSCQDLSTD
                     GYFSLAMRLTTEVNNTEFGQYLTAHRGDTVKLTTLRELIKRSFGENNTGKVTLSCKNG
                     ILVDIYVQLPALIPFKESLESLVDKASHSQHHDYCNSNVTISDFSNEKWL"
     gene            20272..21234
                     /locus_tag="U6309_001083"
     CDS             20272..21234
                     /locus_tag="U6309_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEN5504536.1"
                     /translation="MLKRCYLLILLMFVLASCAHHKPQTPPAEVKKQGTSSTRQFRQV
                     SSFNQIVVQGRLNVNLHTGYNKPEVMLRGDPRDLVQVRTIVKQNTLYVSLGQGYPDYG
                     AVTVDIKTKFLNRFRYEGAGVVTGNNLRTSYLDLYLANEGTTRLAGNIGLQKLEAVGN
                     GVTQINGVSSRNLQIVLKGDPKVLISGFVNLRQLDMYGKGTLSLYWIKSDTLTIRAKK
                     AAKIQLAGIVNRLDVELWDFAQFKGKYLRAQRSFVKTHDKSVAEISAVNHQSSLATDA
                     SDIYYYNLSKTRADFMAFNGSVLDMREWGQSDLKDFDRYNKQFP"
     gene            complement(21302..21535)
                     /locus_tag="U6309_001084"
     CDS             complement(21302..21535)
                     /locus_tag="U6309_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504537.1"
                     /translation="MFFYNASLKNDELNASKIAVLVTGLASLLLIGNYSDEESYEDVS
                     PSFLSRMMEDTPNYPFTSDEEGFEVNIGIPFQL"
     gene            complement(21682..22467)
                     /locus_tag="U6309_001085"
     CDS             complement(21682..22467)
                     /locus_tag="U6309_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN5504538.1"
                     /translation="MKINDFRYMRRGKHLSELQSEEANLLAPVNQRGKGTERALLVLH
                     GFSSSPAVYRYLIPNLKNYDAILCPVLAGHAESIEAFSKSKAADWLQSAKSACELLIN
                     QYQKVDVLGLSLGGLLACQLSQSYAINHLFLLAPALKLHMRINAMLKLAQILKCLGFR
                     QLRNAAGNIITDEHAEIAYRKLPISTIIEMLTLAQGYEWLPPTCPADLFLGIHDQVVA
                     SQQVEQLFCNLPNVKIHWLKNSAHVLPLDNDLNEIIDCINKSK"
BASE COUNT         6806 a         4111 c         4848 g         6775 t
ORIGIN      
        1 ggatcaacat ccacattcca gcggatgcca ttacttaatt tattcattgt taaccactct
       61 cttaattgcg tcaatgaact ttttagaact tttcttgaag gggatttaat taatagttgc
      121 atcctatgct ggttagcttt tcgtggtaaa ggagctggtg caggacccag taccgtgaga
      181 ggatgagctt gaatttgatc tttggttgct tttaggaatt gctccacctt gttgacggtt
      241 ttgccctgag ctctgatgac cgctaaaaac tgaaaaggtg gcatttcagc ctcttgtctt
      301 gaaattaata aagcgttcgc aaattcatca tacccatgct gaatgagtag attaagcagg
      361 ggatgatcag gtaaataagt ttgaatcaat acttgcccgg gatgttctgc tcgccctgct
      421 cgtccagata cttgagttaa taattgcccc aaatgttcaa tagcccggaa atcctggttg
      481 taaaagcctg catcagcatc tacgacaacg accagggata gacgggggaa atggtgtccc
      541 ttggctagca tttgagtgcc tatgatcagc tgagcttccc ctttattaat tttgtccaaa
      601 tggctgttta gagaattttt tttacgaacc tcatctcggt caattcgtaa aacattcgta
      661 tctggaaact gatggcttaa aaactcataa attctctggg tgccagatcc tacggggatt
      721 aattccttac tttggcagtt attgcaaaac aagggtattc tttgggttaa tccacaatga
      781 tgacaaatca tttgtcctgc ctgtttatgc aaggttaaat ggctatcgca tgctttgcaa
      841 tcaaccatcc atccacattg atggcaaagt aacactgggg caaagccgcg tcggttaatg
      901 aaaacaagaa cctggttttg ttgcagcaaa tgattttcta tgattttaag ggttgggaga
      961 gccaagccat gctgtaccat ttgcgatcgt aaatcaatca gttgatagtg taacggtgta
     1021 gtggaggcag ctttatgcgt taagcgtaat aaattatatt ttttttgttt gtagttgtgg
     1081 atggtttcca gactgggagt ggctgtacca agaatgatag gaatattggc aagatgagca
     1141 cgcattaaag cagtatctcg cgcagaataa cgcacccctt ccatttgttt gagcgaactg
     1201 tcatgctctt catcgatgat aattaaacca agattgggta gaggggtaaa aacggcagaa
     1261 cgagttccaa tcaccatttt aactttgttc tcttttgcca gctgccaggc aatttgcctt
     1321 tctgattcat tcaaattgga atgtaatacg gttatggggt ggttaaatcg ggctgtaaaa
     1381 cgagataaaa gctgaggggt taacccgatt tctggtacca gcaccaatac ttgtttgcct
     1441 tgctccaata ttttggcaat gacatgcaaa taaacctctg tcttaccact cccggtaaca
     1501 ccttgcagta aaaagcattg gtaatgatgg atgtgttcga aaatggaatc tacggcaacg
     1561 gcttgttcag gattgagtgt tagcggtgga ctgagttttt caggaatttt ggttggcaag
     1621 gtaacttgct gtgacaggtt aattgcttga caagcaagta gtgccgcaat ttgagttccg
     1681 ttgtagccat gttgttttag ggtctgtttg gaaatccctt tctggtgcct gcttaaaaaa
     1741 tcaatcaact catgttgttt ttttgctttt ttcgagatga gtgtcttcgc cttttcaaca
     1801 ggtatattca attgataaaa atcttctgta ggtagtgagc agggtaaacc caggcgatat
     1861 ttttttggta ttaccaaagg aatcacttca gataatggtg actgataata gcttcctacc
     1921 catagacaaa gctctaagag atcttcagga acgagaggtt tctcatcgat tattgaagtg
     1981 attttcttta acgaatggcc cgtttgctgg tcacaaactt tattgattac aatgcccact
     2041 cgggcttgat tgcgaaaagg aacccatact cttccgccaa tgcatggagt taaatccagg
     2101 gcttcgtaat caaaacaatc tcgattggta tgggggatac aaacctgata aatatcgttc
     2161 atttaacttc taagccattc atgactcgct gattggccgg gcgcggaaaa cactttgact
     2221 actacttttt cgatacgatg attatctaac tcctcttttt ctttaatcag ttcattaaca
     2281 aaccagcgtg ataactcttc cacggtaata ttggttaagg gcattaaggt gatatcttct
     2341 ttaagaaaag ggattttttt cttattgaag atgaaataat aatattcttc atcttccgag
     2401 tattctaaaa aaggtgaaaa ttgtggcata agaaaagttt gatttaagta gcggcataat
     2461 ttatgaatcc gctctttgta ataacggtaa tcaaaagtca taccattttc ttctacccag
     2521 gtagttagag ccaaataaac agaatacata tgaccatgca aaggctccct ttctgttgct
     2581 gaaaagatgg tggtatgccc ggcagaaaac ttcatggatt ctttctgtaa ttccacagta
     2641 gtcagatatt ttttcataat tataaattca ctcgcttatc atttatcaga aaaccattta
     2701 aatcaatttc cagatctttg gttaacgcaa tgactttgaa tagctctccc atttcactcg
     2761 gttgagttag ctgtttaatg gcctgttttg ccgcaaacag ttcctgttca tcatcaagag
     2821 tatggattaa gcttagcaaa ccgttcgcca gcaaaaaaga ggcttgatta gtataccctg
     2881 caacatgaaa tcccgcctgg tgccccgcct cagcaacatg agtaaaatca acatgtgaag
     2941 tgatatcttg ttctcccggg ttaagtaaag gattgggatg agaataatgt tgataatgac
     3001 acattaacgt gccttgatta cgatcagggt gatagtattc atggcgaggg aagccataat
     3061 caataataaa caaagcccct tttttcaaca tatgactgca attcaacaac caatcatcaa
     3121 gaaacaaatt agcctcagta aggtaaggat aatccagtgg tggtaaatga tttttgatat
     3181 aggccaatag tcgttgattt tgtactggtt tgaagatttc tgctaattga tcatgttcat
     3241 ccagagcgac atagctttcc aggatttctg tttcagattg cataaagcga taaactggca
     3301 tggcatccag tacttcattt gctattatta ctccattaaa aggagcttct ggccatttgt
     3361 ccagccagtg aatcaagccg gccaattgcg ggattttttg ttgaatcaat tcttgttgac
     3421 gatggcgtaa gttagcactt acttccagga tgaaatatga gtcaggcaag cactgtaatt
     3481 gttccaaatg agttaatgta tcaatacaga gctttccagt tccggcccca aactcgaata
     3541 gtacggggga gtccagcaag ggcagaattt gttggaattg gttggctaaa gttttgccgt
     3601 acaaggaggt taattcagga gcagtgataa aatcaccgtg ttttcctaac ttttgcagtc
     3661 ctgaagtata atagccctca tagggggcat agagtgcttg ctgcataaag tctataaaag
     3721 ggatttcttt ttgcagcttt aactggttta ttagagtttg ttttaaattc ataatcactt
     3781 aaactatcgt tagacttctt ttaaactcgc tgtgaacaag agggtgcgag gagaaacaaa
     3841 gaaaatactg aagtgtacac aataatactt gaggattcga gcatcctggc tgattacttt
     3901 ttgcagtgag gttgcgaaag gagtctgtta ccatatgagg aacgtagctt gaatcagaca
     3961 aacacgcaag aagctaaggt tgctttagtg actggaggcg ctaaacgtat cggcgcagca
     4021 attgttaaaa aattacatct ctctggatat caggtagcca tccattgtcg ttctgctttg
     4081 catgaagccg atattctggc caaacatttg aatcaggtac gaatgaatag cgccattgtc
     4141 ttgcaaaaag aattaactga gcctggcgca gcagaggaga tcatatccaa agtgaaacat
     4201 tgggcgggtc gtttggattt gctggtcaat aatgcttctg tttttaaacg aacggattgc
     4261 cattttttga atgaggcaga ctggcaatat ctgtttgcag tgaatgtcca ggcccccttt
     4321 ctactgagta tagcggcttg tcctttacta gctccatgca agggttccat tattaacata
     4381 actgatattc atgcagataa gccacttaaa ggatattcgg tatattgtca aactaaagcc
     4441 gcattggaga tgcaaacgag gtcgcttgct cgtgagttgg cgccagaaat aagagtcaat
     4501 gccatcgcgc ctggcgccat tgcctggcct gagaatgaca atagtttaac tgcggaagca
     4561 caacaaaaaa ttatcgagaa aacaccatta aaaagccatg gcaaacccga ttttatcgct
     4621 caggctgttc ttgctctggc tgaaaattca tttatcactg gtcaaacatt gaaagtagat
     4681 ggaggtagaa gtttgacaag ctagggttgt atactggatt tcatttcaaa tgctcaattt
     4741 actatatagg gattattatt atgtcaaaat ggattttatt atctggctca ctttttttct
     4801 tcttattctc tttaggtgtt cactccaatt cttttgataa agagcagtta gtgcaaagat
     4861 gccagattct tcatgaggaa ttaaaggagc tggaatctca tcagtacaat ggggtatgtc
     4921 ggcataaact ggcattagct gcaaataaga tgttctctgc aaaaattaga attgtttatg
     4981 agaattacaa aggcgctaaa caagatcttt cagtatcaat gaataatatg aaatttgcag
     5041 aagatatttc ttgtgttttt aagtcagaga ttaccaaggc cagaatggaa gcccgagaaa
     5101 ttcagcgtga gctgaactaa atagcggaat gtaatggtta tcaaattata gaaataatat
     5161 acaaatcctg accttatatc atgaggcggc aactaatatg ggggaaactc ggtgagttgc
     5221 ctcagctaaa aaatccttga tttccattaa tgaatctaat tgatagaatt gttgcagttg
     5281 ttgttcattc agttgctttt tgaagtaata gcgaaccaaa gatttacttt gcagtacggc
     5341 tttatgctcg ttttcaaggt atgacaatcc ttcgaggtgg agcataaaca gttttaattt
     5401 ctcatcaaaa gcgacatcat ttgtttccag attcaataaa tcccgatata gccagggttt
     5461 gcccgtacct gctcgagaaa tcataaaacc atcgcacgcg ctgatttcca gggcctttct
     5521 taaactgaat atattgttaa tatcaccatt tacaatgact ggtatattga cagcattctt
     5581 gatatttgca atttgctgcc aattgcatgg gatatcataa tcttctgtcc atcgcctgcc
     5641 atgcactatc agggcatgag caccagcgtc ctctatttgt ttggctaagg ttaaatcctt
     5701 ttcattgttt tgaatgcgta ttttaatagt cagcggaatg ctgatcgctg cgcgtaccct
     5761 agagataatg gcaatcaatt tttgtggatc ttccaataaa gcgctaccag cccctttttt
     5821 acgaattttg cttttagggc agccgcaatt aatatcaatt agatccgcac ccagtgattg
     5881 cagtttagct gctgcttctg caaggacatg tggctcattt ccggagattt ggtaagccag
     5941 gaatttttct gtgggagctc tgtaaatata ccgggattga gaggaatgtt tatgaagaat
     6001 gtcgatggca gaacacattt cagaaacgca ataagcaggc ggtgtataaa tattgaatag
     6061 atctctgaaa ggggcgcaac tgtatcctgc caaaggtcct tgaatcagtc gatgagggag
     6121 ttttaaagag ccgatttgca gaggactgtt aataaaagtg tgcatcaatg gaattaaagg
     6181 ctataatcgc tttttatttt aacatgattc atcatgaaga accagaaaaa agggagaggc
     6241 ttccatatgg acaggaggta tttgtcacct ttggagctgc taggcattgc tacgcaacac
     6301 gcctatacag cagattatat gttgcagcaa attgccaatg gaatgtatcg aggtggggaa
     6361 accatcgcag ttttttctcc aatcacttca ctcatgtacg ttgcttttca gctaaccttc
     6421 aaagcttatt gtttgcatga tcatagacca ataaaggaat ataaaaatct gatggaattg
     6481 gttgagttga atagtcatct tggtttatcc agcaatgata tttttctttt aaaaacttta
     6541 tcaagacagc aagtgtttaa taaaggagta gattacgacc tttgggaaaa tcagcaacaa
     6601 ttgcatgttt tttgtgagga aattatttca ttgtatgaga gagtgcagtc gatgatgcct
     6661 ctggagttgc aaagtgacta tcaggagtaa ccggctgaat tagcgccctg ttaagtgtct
     6721 ttgcttaagt ttccttatgt tgtgcatagc ccaagatgca ttaatcagat atcaaaatga
     6781 tttattttta tccaaaatag actcattcta cttggcatcc caatggattc aagttaggat
     6841 attatttttt taaaagggta attgttatgg agcaactaga cgataaagtc gcagataatg
     6901 tgcaacaaaa aatactacag ttaagtcgtc atcttaattc gcgtattttg ggtcaaaagg
     6961 atttgatctc tcgtctgttg atcgctttgc ttgcagatgg ccatttgttg gttgaaggag
     7021 ctccgggttt agcaaaaacc agagccgtta aagaattgtc agacgtagtt gaaggtgatt
     7081 ttcatcggat tcaattcacc cccgatctgc tacctgggga cttaactggt acggacgtct
     7141 accatccgca aaatggttct tttgtctttc agcccggccc tatatttcat catcttatcc
     7201 tggctgatga gatcaaccgc gcccctgcca aagtacagtc agccctgctt gaagcgatgg
     7261 cagaaaggca agtcactatt ggaggaaaaa cttatcctct gccagaattg tttctggtca
     7321 tggcaactca aaatcctata gaacaagaag gaacttatcc tttaccagaa gcccaattgg
     7381 acagattttt aatgtatgtg aaaattagtt accctgatgc aaaagtagaa catgacattt
     7441 taactttggc acgtaaagaa gcactgggta atgcaacaga aaatcaaatc aaagtgacga
     7501 aattgtccca aaaaacttta tttgaagctc gaaaacaagt attgaaagta cataccagtg
     7561 aagcacttga aaattatctg gttcagttgg tagttgctac ccgtaatccc ggagtataca
     7621 gtgacgaatt gggacgctgg ttgcgttttg gggcaagccc acgcgctact atcgcactgg
     7681 accgttgttc caaggctcat gcctggcttg caggtagaga ttatgtgact cccgatgata
     7741 tacacgttat cgctcatgat gtactgcgcc acaggatttt gttaagtttt gaagcagaag
     7801 cagaaggcat tagcagcgat gactttatag actctttatt acgtttagtc gccatccctt
     7861 agaggttttt tatgactgac ggagttgtag ctgaattaag cgaattaatc gatttaagac
     7921 ggtatgttca ttccgttcat tataagccgc aaggaagagc gttgcgttct ggaaaccata
     7981 tttccaagtt tcgtggacgg ggaatggatt ttgcggaagt aaggaattat caggctggtg
     8041 atgaaattcg tcatatggaa tggcgcgtta ctgcgcgtac tggaaggcct catgtcaaaa
     8101 tttatcagga ggagcgggaa aggccagtgg ttctgcttgt agattttaac ccttccatga
     8161 ttttcggtac caagatagca tttaagtcag tggtagctgc acgattggct tctcttttag
     8221 catggacagt gataaaacaa ggagatcggg taggaggatt ctttttttca gctacagaac
     8281 acagtgagtt tattccgcga ggacgagatt cgggcgtact ccctttattg gcttctttaa
     8341 gtcgatatac cactcaaaca gaggttcagc gagaagaaaa gccacgtatg ttaagtgatg
     8401 ccttaatgag gctgcgtcgg gttattcggc ctggaagtat acttgttttg ataagcgatt
     8461 tttattctat ggatgttgag tgtgagaagc atttaaatcg tttacgaggc cataatgaca
     8521 tactggccta tcatgtttgt gacagaattg aactggctcc tccaaaaccg caacaatatg
     8581 ccattagcga tggtaaacag gaaatcatat tagacactgg cctggaaact gttagcacag
     8641 cttatcagct ctattgccag cagagaattg agcagttaaa tgatcaattt aagcggttgc
     8701 aaattcagta tgtccaggtg actgctgata ccaatctcac tccactggtg cgcttgacct
     8761 ttccccggag gtctcgtggc taaaacagaa ccattagctc aattacgaga cattcatttg
     8821 cctgagcctg ttggctggtg gcctcttgcg ccagggtggt atggcttaat ggttcttatc
     8881 gttgttttag tggcaggggt tgcctatttc atttataaaa ggcatgttaa cgcattaccc
     8941 aagaaacaag ccttaacttt attaaaaatc cacaaagaac agtatgaaaa agataaaaac
     9001 actcaattgg ccagtgccca tatttctgaa ttgctaaagc gagttgcctt ggtttattat
     9061 ccaagagcag aagtcgctag tatgcatggt gaagcttggg ttgaattttt aaatcagact
     9121 ggcaagggga ttgattttac tccggttaaa tcgatgttat tggattcacc ctataaaaca
     9181 tcagatgcac tgaatttaaa tcctttattt actcgtgcag aaaaatggat caagcaaagg
     9241 gggaagccgt gttcgaatta gctaccccat gggctttgtc acttattcct gtccctttgt
     9301 ttctctggta tttgttaccg cgggcaaaag taaaattacc tgctgcattg aaggttcctt
     9361 tttttgaggc aatggcaggt attgttgaac aagagaaccg gctaatctcg gctaaaacgc
     9421 tgctattaat tcctgtttta gtttgggtat tgctggttat cgccctttct ggtccgcgat
     9481 gggttggtga gccaaagcct gtagcaaggg aaggatataa tatcatgatg gtattggatc
     9541 tttcaggcag catggaaatt acggatatgc tactgcatgg ccgaccagtc agtcgtttgc
     9601 ttgtcgtgaa aagggctgca gagcaatttg ttgaggacag agtcggagat agaatcggtt
     9661 tgattctgtt tggtactcga gcttatttgc aaacaccatt aacctatgac agacatagcg
     9721 tactaatgag gatagacgac gcaacagctg gtcttgcagg taaaactact tcgattggtg
     9781 atgcagtagg tttagccgtc aaaaggttac aagatgttcc ttccaaagga agagttatta
     9841 ttttgctgac ggacggagca aacaactcag gtgtattagc ccccttaaaa gcagcagagt
     9901 tagccaagca agatggaatc aaaatctata ctataggctt aggttcggag gcagatccaa
     9961 gagcgttaac tggtgatttt tttgccccca ccctttccgc cgaactggat gaaaagacat
    10021 tggaaaaaat ggcaaaaatg acaggtggtc gttatttcag ggcgacagat cctgagtctt
    10081 tacaatccat ataccaaacc atcaatcaac tggaaaccgt gaagcaagaa caggcaactg
    10141 ttcggcctca aaaggaatat tatccctggt tcgttgcctt tgctttgtta ttatgctttt
    10201 attggttgat tagcaaggcg gatttatttg tgtacttaag gtctttttta aaagtagagc
    10261 ggagcttaag tcatgatagc tgagtttcat tttttaaggc catggtgttt gttgatgata
    10321 gtgcctttgt ttgcgttggt ggtaatttat tggcggcaaa aaccaaaatt acatgcctgg
    10381 gaggcggtat gtgatcccca tcttctggaa catttattac acagtaaagg tcagagtaaa
    10441 cgtgccttat ctttatcatt cctgtttttt agtattttat ttatgatttt gagtgtgtct
    10501 ggtccaactt ggtacaaatt gcctcaatct acttataagc caattcagcc aagggttttg
    10561 ttattggata tgtcagataa aatgatgctt ggggatttat cgccaaatcg tcttagtcgg
    10621 gctaaattca aattgcatga tttatttacc cgcaaggata tagggcagtt tggtttgatt
    10681 gttttcacag gcgaaccttt tgttgtatct cctttaacag acgatggtca aactatctct
    10741 tccttattgt cagccttgac ccctgatatc atgcccgttg caggatataa tttagacagt
    10801 gccttggatg aagcaagcca attaattaag caagcgggtt atgaccatgg cgaaatttta
    10861 gttttaaccg ctgaaacacc gtcctctagc gctattgaaa aagctaaaaa gctggcagaa
    10921 tcaggtattt tttcttcaat tatgccaatc agagctgatg aaaatttgaa ccccttgttt
    10981 cagcgcttcg ccaatgcggg agatggatac ttgttggtat actcaccaga ttcaagtgat
    11041 ttggaaaagt ggttgaacgc ctctcaaggc aaagaaaaat ttgctcttga aaaaaatgaa
    11101 gatatcccat tatggcgtga tgaaggccgc tggttcttga ttcccgcatt gcttttatta
    11161 ttacccgtat ttcagcgagg ttggatgcaa agggtgacga tatgatgtgg cgggttttaa
    11221 ctattgtggc agctgcacta atatccaatc aacttaaggc atttagttgg tctgatttat
    11281 ggtttactcc caatcaacaa gctcaacatt tgatggaaaa gggacaattt gcaaaagcta
    11341 aagatctttt tgagcgcaat gactgggctg caagcgcagc atacagagca ggagattatg
    11401 aacaggcagc caaattattc aatgagttaa aaacggaaca aggctactat aatcagggaa
    11461 atgctctagc acacttaggg aaatatgagg aagcaatacg agcatatgac aaagccttgg
    11521 catttaatcc aaataatcag gatgctttat ataatcgaaa attaatcagt gaattgttga
    11581 agaaagataa agaaaagaaa aaaagccaga atcagcagga taaggatcaa cagaaccagg
    11641 atcaacaaaa tcaggacaag caaaacaaag atcaacagaa ccaggatcag caaaatcagg
    11701 acaagcaaaa caaagatcaa cggaaccagg atcagcaaaa tcaggacaag caaaacaaag
    11761 atcaacagaa ccaggacaag caaaatcagg acaagcagaa caaaaatcag cagaaccagg
    11821 atcaacaaaa tcaggacaag caaaacaaag atcaacagaa ccaggatcag caaaatcagg
    11881 acaagcaaaa caaagatcag cagaaccagg atcagcaaaa tcaggacaag caaaataagg
    11941 cacaacagaa taaagttcaa aataaaaaaa atgacaagca agagggagaa attcagtccg
    12001 aagcgaatca ggagaaacaa cttgctaaag aacaatggtt gcgattaata cctgatgatc
    12061 cgggtggatt aatgagagaa aaatttttac gtgatcattt acgtagagag cgtgggtggt
    12121 atcaatgaag aaattaatgt tatggggttt attgagttta ttttcatcat tatcttttgc
    12181 agaaatcaaa gttgaacttg agccttcgca agtcacaatg ggagaaacct ttaaattatc
    12241 cattactcaa gaagatcaac aggggggcgg agttcccgat ctgactgaat tgcaaaagga
    12301 ttttgtgatt ttgggtacag aaaggcatgt aaactattcc attattaatg ggcaggcgca
    12361 atcacaaagt caatggattg tactcttgag agcgctaaag ccaggtattt ttactattcc
    12421 tccaataaaa gtaggtcttg aacaaagtct gccaatcact atcaatgtcc agggtagtgc
    12481 caatacacaa gctttgccag atgaggctat acaaaagcag gacgttttcc tgacagctga
    12541 agtgaatgag aaaaacccct atgttaacca acaaattatt tatacggtca aattatacaa
    12601 tagcaaacga ttgttagatg ccgattatca aggtccaaaa gttgatgatg ccttgctgat
    12661 tccgttaggg gatgcgaaac gttaccatgc tgttcataac aacattaatt atgttgttga
    12721 agagcaaact tatgcgatat atccgcaaaa aagtggcact ttgaaaatca tatcaccgat
    12781 ttttaatgct cttgtatatg actataaccc ccaacgcacc aaagcgacgg ataaagcgat
    12841 taacttaact atcaagccaa tccctaaaaa gtatcaaggc aaattatggt tgccagcaaa
    12901 acaggttaag ttatccgagc aatatgaaaa cagtaatcag actctcagtc agggaagtac
    12961 gttaacacgt acagtgacat tggaaggaat aggcattcca gctcagttat tgccaacact
    13021 tacctttcaa gaaacagatt tctttagcgt ttatccagag aaaggggcgg aacgcaatca
    13081 ggttaagcaa ggtgagctaa ttggcagtgt cgaaatcaag gtcacttatt tgttcaacaa
    13141 agcaggcaaa acgacaatac ccgaattgcg tcttacctgg tttaatactg aaacaggtaa
    13201 agaagaaatc gctgttttac ctccaaggag catagatatc atcccttcta ctgcggtcaa
    13261 ggcaaatact gcaaacactc aagtcacagg taaagaaatg tctgccagca gtcaattagc
    13321 gcctgattct gcatcagttg ccaatgaatc aggaggttgg gctgtgaagt tggcaatatt
    13381 ttttggtttg gcctggttaa tcactttaag cttatggata tggcaaaagc gaccgagaga
    13441 gtcatccagt aaaaaggagt ataaaaaagc tttggatgct ttaaataaag cttgtagtca
    13501 atgtaatccc aaaaaagctc gtgatgctgt tttaaaatgg gcctctttgc aatggcctga
    13561 tgctccaatg cttaatttaa ctgacttggc gcaattggta agagacgcgc atttaaggaa
    13621 acaattgcat ctgctttcgc aagtcttgta ccgaagtcag gaaaaaacat tatggcgcgg
    13681 ggatgaatta ttgcgggctg tgcaagcggt aaaacgtaac gaatctgtga aaaagcgaaa
    13741 aaacagtaat ttacctccta ttaatccatt ttagaaatta aatctgttta agcggtggtt
    13801 tttgccagtt attcctttac tgtcaatatg gacaacttat ttatcctctt ttttctgtaa
    13861 tcacgggact actgccagat tgtatgccgt cataatttgg ctaagctggc tcaggcgact
    13921 atgataacaa cagctataat gttatagata accttgtaaa gagtaatgtt ctctctttat
    13981 aaagctctct ggctattgta ggaattgagt atgcatgtta atgagtatat ccactatgat
    14041 gtgcaggagc ttgctgaact gatcaaaaac catcaggttg gtgctgagga agttttgcgg
    14101 tgtgcgcaag aacgcctata tcaagtgaat ccttcattaa atgccgtagt tacagattgt
    14161 tttgaatatg cgcatcaatg ccttgctaaa atgaaaggta acgagcccta ttatggtgtt
    14221 ccgttattag tcaaggatct gggccatgcc atagagggca ttcgactaac ggaagggtca
    14281 aggttttttt caaataatct ggctgagaca aacagtgatt tgattaataa attgattgct
    14341 cttggttttg taccatttgc aaaaacaaat acttcagaat taggcctttc ctgcgttact
    14401 gactctaatt tatttgggcc ttgcagaaat ccttttgata ctatgagaac ggcaggcgga
    14461 tcttctggtg gttctgctgc tgccataatg gcagggatag cgcctgtggc tacggcaagc
    14521 gatggaggcg ggtcgattcg tattccagcg gcttgttgtg gtttgtttgg attcaaaccg
    14581 acaccaggtc tggttccctc agggccattg gttgatgaag catggtccgg gatggctacc
    14641 agttttatac taactcgttc catgagtgat gctattgcat tgtttaaaaa tgtaataaac
    14701 cccatgcgac ttaacagact atcgagaaag aaaaaaaaat tacttattac cgagttgaca
    14761 ggcgcttttg cagaggtacc aattgcaagg gaatgccagg aggcggtaga gaaaataaaa
    14821 gaaatcctga aaaattcagg tcacagatta aatgaagtgc acttgccatt ggatctgcaa
    14881 gttatcagtt catgcgccca tactttaatt gcagcgagca cgtatgcgaa aataaaaact
    14941 caagaacaaa tcagtggcag aaaaccaatg attcatgaat tggaacccat aacatgggaa
    15001 ttttattaca gaggaaaaat ggtctccgct tatgaattca ttattgccaa aaacaaacta
    15061 tatcaatcac taagacctct gcatcaatta ttggaaaaaa cagacattgt attaactcct
    15121 gcattggctc aattacccct gttaatcggt catttgcgca ctgatgagga attcaataag
    15181 tatctacaaa aaaagaaaga gttttcacct tttacttccc tgtttaatca aggtggctta
    15241 cccgctatga ctgtaccagt tatgttccac catcatttgc ctgtctctgt gcaaatgggt
    15301 tcagcaaaag gcaatgattt gctcttattg gagttggctc atgaattaca gtctgtgctg
    15361 ccaaaaaata tcacgcctgc atcaattcct tgatgctgat tttttgtaag ttttcattta
    15421 tcgaaccttg tgttaagtta actgtagctt ttcataaaat taataataag tgattgtttt
    15481 tttatagcta tagtcacttg aattttaaat aatcgattct ctacgtactg ctgcttaacg
    15541 cggtttttaa aaatcaagtc actatatatt ttttcaactt cagggcattt tgcgtaggca
    15601 gtaaatagtt ccagtttgag tagttgtatt taataaaggt aagttgtaaa tggatttcac
    15661 caaagaggat ttcaatgaag accttaccat ccaaagtcat ttttataggg gcaggcccga
    15721 gcggcttatt cgctgcacgc caattaaagg aaatagcaaa attacaaaat aagacagtag
    15781 aatgtatatt ccttgaacgt gaacccgtag tgggcggcaa atgccacacc tactcggatg
    15841 cagctcatcc tgagttaaaa acggaatggg gagccgcatt ggttgctccc aactatggtg
    15901 ttgttcttga tgcattagct gagcacggca ttgaatttga aaaggtaatg ccaacagctt
    15961 ctgaaacgat agaaatcaag caattatttg aaaaagccag ttcgttagaa aaaataagca
    16021 tggaaatgaa gttgcttaga gaattatggg cttttaatag tgattatgat gtttataaaa
    16081 aggcaaaaca aaataaaaaa gcacttccag atcaattgtt aaaccccttt tctgaatatt
    16141 gtaagctgaa caacatgtct tatttacctg tccttttaaa gccatttgtg cctggatttg
    16201 gttacgggga tttaaacttt tgtccggctt attgtgttct tgagtatatg ggcaaggtca
    16261 ccatgccgga catcctctta gccgatcaat taattggcag gccctcacta ttagcaatac
    16321 atggcggttt tcagctttta atggagagaa tcgcggaaca atttgatgtg cgtaccagtg
    16381 cttctgtttc caggattaag agagaaaaaa ataaggtgac tgtgacttat gtgcataatg
    16441 gtatcgagca aacagaaaca gccgatactt tggttttggc gacttcacca aaaaactgga
    16501 aatctctggg tttagactta actgaagttg aaaacaattg tatagagagt ctggattatt
    16561 atcgctatcc tgttgctgtt tataaaatca aaggattgcc gccgcaacag tattttttca
    16621 caaaagcact tgaggaaagg ggctttgggc atttggcgct tattacaacc agggataaca
    16681 ggagtaatcc ggaagatgga cggttatgca cggtttatgt gaatttatct cccaataata
    16741 atgaatttaa atttgatcat gaagcgctaa gaaaagaatt agagtctatc ccggatgtaa
    16801 ctgaagttac ggttgtaaag gataaaatct gggaagatta tatgtcgact cttccctggg
    16861 atttgcgatt gcaattggat aaagagcaac agcgcacgaa tactatgtat ttgggctctt
    16921 atgctctggg aagttttgaa gacgtagcct gtgtggcaaa taaggcaacc gatgccatga
    16981 cggaatggta tgctcccact gtgaggtatg aagaggattt cagctggaaa aatatgaata
    17041 gggcctggca gttttttaca tcgccaacga aaccgccact cgcttcgttg ggtgagccca
    17101 gggtgtcgga tgagcacagt aacaggccat agacggtaag ataaaagtgg tacatttatc
    17161 gaagatattg tacataatgc tcgctatttg tagaatagta tattataata taattacttg
    17221 ttctacgagc attattggtt aaaatcttgt ttttcgacaa actccttcct tatatttccc
    17281 ttggtcgtga atttctcatc gatgattaca ttgtattttt tctcttatcg ctcattacaa
    17341 gtgtttgttt aattttattt ggaactttga aatgtctgtg aaagaaactg gccacgttaa
    17401 gtggtttaat gatgataaag gttatggctt cattagtcgt gataatggtc aagacgatgt
    17461 atttgtacac ttccgctcta tcgttagcaa cgctgggcgc aaaactttgc tagagggaca
    17521 acgagtggag ttccttgtta ctaagggagc gaaaggtttg caagcagaag aagtaactgc
    17581 tatataatcc cgtattcact gaattacctt cctctttgtc taaaaaaacg aagaggaagg
    17641 tggtttttta atgccaataa attgcattaa ttttttcaat tatttttcac tttctcttaa
    17701 gaatttgtac cgagacctgt ttggtataaa agctggcagt aagataacga tattccactt
    17761 tattttcttt tacccattct tggaaagctt taaattcatc ctggcgccaa ttgatattac
    17821 caatgtactc atcgaatact atgatagtac ctggtgcaat ctgggggttt aattcgttga
    17881 acaccaattt tgtggaagaa tagagatcac aatcgatatt cataaaagct atgggttccc
    17941 tgttattttt tttaaaatct ggcaaagttt cattaaacca tccagcgatt aaagtcacat
    18001 tacttgctac ggagggaatt ttaccatagg tggaataaga gccagccttt tcgtggtgcc
    18061 aatcttcagg taacccttga aaactgtcaa aaccgaaaat tttgcgtttg ggaaagagtt
    18121 tggcaatatg ggaaatggaa tagccaaatc gcactccaaa ttccagtatt aagccttcag
    18181 aaagaacctg tggtgctaat aaatcaaatg tttgataagt tgttccacta tgtcgcaaat
    18241 gtttgtcagc atgtttatga agataatcag ctgcatcaat gagatcaaag cgtttgaatg
    18301 cgcgatacgt ttctatcggt gtcttgttta aaccattgag tatgtctatt gcaattcgct
    18361 ggcagcaaaa ataatcatta ttgggtatgc gttgaattgc tctgtctaaa agagatttta
    18421 ctgctttttt atcatttaaa gccagatata ccgcatgcag taaaatggtg atgtgtccat
    18481 ctttatccgt tttttcaagt ggttcaagta agaagcgcgc ttcctctact ttattaaggt
    18541 caagcaaaat aatggctctt gcccaacttg ttttggggat attgaattgg tttacataag
    18601 ggaacaagga atacgcttct tgtacccttc cttgttgcct tagagcatag aggtaatagg
    18661 cgaggctttc accgtccatt tcttcaattt taaatgattc aaaaagcttg caggctgaca
    18721 aagaataatt agcatgcgcc agagtttttg ctgtcattaa tcctatgtca gttttgaatt
    18781 cgctgaataa ataagcttgc cagtacatgg ctgctgcttg tattgtttta cccatgcgaa
    18841 gtaaaacggt tcctttttga taataggctc cggaaaattg tgggtttatc gctaatgctt
    18901 catcatacag tgttaaggca agaggcaaat cccccttctc taaagcctgg tctcccttgg
    18961 atttaatcac tacaggatct tgttgggtca tttattttct ccagtaccta cctctatatc
    19021 atagtattct tttattcgga atgtagttgt attgaaaaaa tccttattaa tcaatttcat
    19081 gaaaaaataa ttgctggcat tcatatccga ctagggtata atgggcttac caaagacaag
    19141 ccaggatgtc gtaatgataa aaccaatcac cctgcttttg cttttttgtt ctttcactgt
    19201 taatgcctca attctcgtca atggtacttt cgaagcaaca aaatcatgcc ctgcttacct
    19261 ttccaagaat cgtaaatcaa atcctgataa tttgatggtt gagcccaacc agaaatatca
    19321 attaaaagaa attaataaaa ccaaaccgga ttggttgcgt atacaatttt cagatcatca
    19381 acctaatctg cgctgggtga gtgttgattg cggggtcatt gaatacactg agcgtcccaa
    19441 agcgagctgt gatctttctc ccgggatggc tgattcttat gtgttggctt taagttcaca
    19501 gccaggtttt tgtgaaactt atggatatga aataggcaaa ccagagtgtt tgaaattaac
    19561 aaagacagtt tatcaggcca atcatctgat cctgcatgga ttatggccta atcaagaagc
    19621 atgcggccat aattatggct attgtggaat aagccctcga acaaaccatt gtgattattc
    19681 tccattggat ttatcttctg atgttgctca aaatttaaag aaacttatgc cgagttacaa
    19741 ttatggcagt tgtctggagc gccatgaatg gaacaaacat gggagctgtc aggatttaag
    19801 cacggatggt tattttagtt tggctatgcg cttgactaca gaagttaata atacagagtt
    19861 cggtcaatat cttaccgctc atcgtggtga tactgtaaaa ttgactactt tgcgtgaatt
    19921 gattaaaaga tctttcgggg aaaacaatac aggtaaagtg actttaagtt gcaagaatgg
    19981 gatcttggtg gatatttatg tccaattacc tgctctcata ccttttaaag agtctctaga
    20041 gtctttggtt gataaagcca gccactcaca acaccatgat tattgtaata gcaatgtcac
    20101 catttctgat tttagtaatg aaaaatggtt ataggtattc aagtggagga gatggctcgt
    20161 tcacaaaact tttttaaaat aatatggcat tgcttcctta taggagactg ccttgcacat
    20221 tgtcaaaact tcctctaatg tagagagatt attgatatag gaatacagat tatgctaaag
    20281 cggtgctact tattgatttt acttatgttt gtattggcaa gctgcgccca tcataaaccg
    20341 caaacacctc cagcggaagt taaaaaacaa ggtaccagca gtacacggca gtttcgccaa
    20401 gtgtcatcgt tcaatcaaat cgtagttcag ggacgactga atgttaattt gcataccggg
    20461 tataataagc cagaggtcat gttacgcggt gatcccagag atttggttca agtaagaacg
    20521 atagtaaaac aaaatacact ctatgtgtct ttgggccagg gatacccgga ttatggtgct
    20581 gtgactgtgg atattaaaac caaattcctc aaccggtttc gctatgaggg cgctggggtt
    20641 gtgacaggga ataatttacg gaccagctac ttggacttgt atttagcgaa cgaaggtacc
    20701 accagactgg caggaaacat tgggttgcaa aaactcgagg cagtcggtaa tggcgtgaca
    20761 caaatcaatg gtgtatccag caggaatttg caaatcgtat tgaaaggaga tcccaaagta
    20821 ctgatatctg gttttgtcaa tctaagacag cttgatatgt atggaaaagg cacgttaagt
    20881 ttgtattgga taaaaagcga tactttaacc attagagcaa aaaaggcggc caaaattcaa
    20941 ttggctggaa ttgtgaatcg actggatgtt gaattgtggg attttgctca atttaagggg
    21001 aaatatttac gagctcaacg tagttttgtg aaaacccatg ataaatccgt agcagaaatt
    21061 tcagctgtga atcatcaaag cagccttgct actgatgcca gtgatattta ctattataac
    21121 ttatcaaaaa ccagggctga tttcatggca tttaatggct cagtgctgga tatgcgtgaa
    21181 tggggccaat ctgatttaaa agattttgat agatacaata agcagtttcc ctagcattga
    21241 ttcaggataa aagagctaaa ttcataaggg taaacccttg gtgaatttag cttgctataa
    21301 attagagttg aaacggaatc cctatgttga cttcaaatcc ttcctcatcg gatgtaaatg
    21361 gataattagg agtatcttcc atcatccgac taaggaaaga gggagagaca tcctcgtagg
    21421 actcttcatc actataattt ccaatgagta ataatgaggc taatcctgta acgagaacag
    21481 caatttttga tgcatttaat tcatcatttt ttaatgaggc attataaaaa aacatgttta
    21541 atttccaaag aacaaaatga gccgttaaaa tacaatgatt ttttttctta ttcaatatct
    21601 aaatcgctat aacgcttgca agtctaaaga agaggttgtt tgattggtta caaatcaatc
    21661 atggcaattt tttatttcca ttcatttgct tttattaata caatcaataa tctcattcag
    21721 atcgttgtct agcggcagta catgcgcgga attttttagc caatgtattt tgacattagg
    21781 taaattacaa aataattgtt ctacttgttg agaagcaacc acttgatcat gaattccaag
    21841 aaaaagatcg gccgggcaag ttggggggag ccattcatat ccctgagcca aggttagcat
    21901 ttcaatgatt gtggaaatgg gaagttttct ataagcgatt tcggcatgct catcggtgat
    21961 aatattccct gcagcgttgc gaagttgtcg aaatccgagg cacttaagaa tctgggctaa
    22021 tttcagcatg gcgttgatgc gcatgtgtaa ttttaatgcg ggggctaata gaaacaggtg
    22081 attgatagca taggattgac ttaattgaca ggctaataat cctcccaggg ataatcctaa
    22141 aacatcaact ttttgatact gatttattaa gagttcacag gcgctttttg ctgattgaag
    22201 ccaatcagct gctttggatt ttgaaaaggc ttctatactt tcggcgtgac cagccaatac
    22261 aggacaaagg atggcatcat aattttttaa attaggaatc agatatcggt aaactgccgg
    22321 tgaagaagag aaaccatgca ataccagtaa agctcgttct gtgcctttac cacgctgatt
    22381 gactggagca aggagatttg cttcctccga ctgcagttcg ctgagatgtt tgccgcggcg
    22441 catataccga aaatcattga ttttcataga atattcattt ttcattgtca tgccatgtat
    22501 ggttgctata gtaattggtt taatttgggg tgaataggct
//
LOCUS       DASLOE010000035        22489 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.035, whole genome shotgun sequence.
ACCESSION   DASLOE010000035 DASLOE010000000
VERSION     DASLOE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 22489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22489
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.035"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1033)
                     /gene="rfbB"
                     /locus_tag="U6309_001086"
     CDS             complement(<1..1033)
                     /gene="rfbB"
                     /locus_tag="U6309_001086"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEN5504539.1"
                     /translation="MICQMLSTILQKSKNLTMKNQMNNLLVTGAAGFIGSNFVKFMND
                     KYPEIKIISLDKLTYAGNKANLSEMAECKNHLFVQGDILDKSLVLSLLREYEIDTLVH
                     FAAESHVDNSIDNPQIFLETNVIGTFTLLEAARIYWLNERQWDKSKCRFHHVSTDEVY
                     GSLEREEPAFTEKNSYQPNSPYSASKASSDHIVRAYYHTYGLPVTTSNCSNNYGPNQH
                     KEKLIPKVVFACVNQLPITVYGNGSNIRDWLYVMDHCEAIDTIIQKGALGEVYNIGGN
                     NELDNLSLIKMICQMMDDLKPMEKPYHSLITFVEDRKGHDKRYAIDNSKIQKELGWVP
                     QGDFVHKLSN"
     gene            complement(993..1877)
                     /gene="rfbD"
                     /locus_tag="U6309_001087"
     CDS             complement(993..1877)
                     /gene="rfbD"
                     /locus_tag="U6309_001087"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEN5504540.1"
                     /translation="MKILVTGANGQVGTEIIKRFSSSEHEVYACTRDILDCSKLERVH
                     DVLSEIKPDLIINAAAYTAVDKAEDEPDLAHIVNAEFVCRLVNYCTLKNVPLIHLSTD
                     YVFDGEKEGAYHETDIPHPVSTYGRTKWEGEQAILSQLKKYIILRVSWVFGEQGKNFV
                     KTILNLASSRKELNIVSDQSGRPTSARDIARVLFEIVQKIRHSSFDYWGLYHYAGQGV
                     TNWHEFANVFLNMAKEKQLLLTLTDLNPIKSQEYPTRATRPKNSVLNTTKIETILGIK
                     SCSWKNDLPDVIDHFTKK"
     gene            complement(1878..2441)
                     /gene="rfbC"
                     /locus_tag="U6309_001088"
     CDS             complement(1878..2441)
                     /gene="rfbC"
                     /locus_tag="U6309_001088"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEN5504541.1"
                     /translation="MNIINTQIDELKIIEPKIYGDERGFFYESFQAKRYEELLGITDR
                     FVQDNFSRSQKGVLRGLHYQSQQTQGKLVSVLAGEVFDVAVDIRLGSPTFGQWVGVIL
                     SGENKRQFWIPKGFAHGFYVLSAMADFAYKCTDYYHPESEFSIHYLDPQLAIDWPLGE
                     QVQLSPKDAAAKLLNLIDAELLPRYQA"
     gene            complement(2531..4033)
                     /locus_tag="U6309_001089"
     CDS             complement(2531..4033)
                     /locus_tag="U6309_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEN5504542.1"
                     /translation="MITIKELYIQKQENLKTALSKLDATAQGVLFLVDSDEHLIRTVT
                     DGDIRRLLLKGFTLDSTLEELSEHSSKALPVSATIQDAYHLMQEYELDHIPVIDESNR
                     PIRLIHRRELSSNILLSSPHIGEHEQQYVQEAFATNWVAPLGPNVDSFEKEVAEYINI
                     KSAVALSSGTAAIHLALVLLDVKPGDIVFASSFTFVATVNPILYQGATPVFIDSDLDT
                     WNMSPVALERALRESKAKNQMPKAVIIVNLYGQSANYDALCQLCNEYNVPIIEDAAES
                     LGATYNNKYSGTFGKLGVFSFNGNKIITTSGGGMLVSEDESLIQRARFLSTQAREPMP
                     HYEHTVVGYNYRMSNVLAGIGRGQLKVLEKRVKARRAIFERYKQALESYPFIDMMPEI
                     TQGYSTRWLSTLVIKPTPNKIKPEEVIEQLKPFNIEARRTWKPMHRQPLFAGSKYYPH
                     DEQFSVSDYLFDHGICLPSGSNLTPRDIDRVIHCLNDIFIKYKQNNFEVA"
     gene            complement(4044..4652)
                     /locus_tag="U6309_001090"
     CDS             complement(4044..4652)
                     /locus_tag="U6309_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN5504543.1"
                     /translation="MHTNLKIIGCGGHCKVVIDALSLTQHSLQVSLCDSNKSLLGQEV
                     CGVLVDSTMDSLADYIGYVHVSIGNNQARKNVIDLMNSNANLFTIIHPAAIIAKSARL
                     GAGSFIAAQAILGPECQVGEGCIINHSAVVDHEVIVGSCSHIAPNSTLGGRVKIGERV
                     LIGAGAVVLPGVTIDDGAIIGAGSVVVKDVKENAVVKGVPAV"
     gene            complement(4642..5775)
                     /gene="neuC"
                     /locus_tag="U6309_001091"
     CDS             complement(4642..5775)
                     /gene="neuC"
                     /locus_tag="U6309_001091"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEN5504544.1"
                     /translation="MTRKIIYVTGTRADYGLMREVLKRLHQSEDIDLSICVTGMHLDA
                     LYGNTVNEIKADQFSICGIIPVDLDNAQHSSMAKAIGHELLGFTEVFESETPDVVLLL
                     GDRGEMLAAAIAAIHLNIPVVHLHGGERSGTVDEMVRHAISKLSHYHFVATEASKQRL
                     IRMGEKEETIFQVGAPGLDEIMQYKTSTRDVFNQRYGFHPDKKICLLIYHPVVQEVDS
                     IKIQFQSVIQAALATNLQIICLEPNSDTGGHLIREVIQEYIDHPDVRIIKHLHRPEFI
                     DCLANSDVMLGNSSSGIIEAASFNLNVVNVGSRQNLRERSDNVIDVDVTYDAILTGLR
                     EALNKPKIKYSNCYGDGKTSERCYQLLKTIPLHSQILNKCNAY"
     gene            complement(5775..6797)
                     /gene="legI"
                     /locus_tag="U6309_001092"
     CDS             complement(5775..6797)
                     /gene="legI"
                     /locus_tag="U6309_001092"
                     /EC_number="2.5.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N'-diacetyllegionaminate synthase"
                     /protein_id="HEN5504545.1"
                     /translation="MTCFIIAEAGVNHNGDLQLAKELVYAAKESGADAVKFQTFKADT
                     LVNKTVEKAEYQKNNAPESSTQYEMLKALELSEEDHYLLSELANSLGIEFMSTGFDEQ
                     SIDFLISLGVKRLKIPSGEITNVPYLQHCASKKLPLIISTGMCDLQEVRVAIDTVKPY
                     YGNSLSDYLVLLHCTSNYPAAYQDVNLKAMQTLADEFQLPVGYSDHTLGILVPTLAVG
                     MGACVIEKHFTMDKSLPGPDHLASMNPEEMKNLVQSIRDAETVLGSGEKKPSDNELPI
                     RALVRRSITLRRDLVKGAQISKEDLILLRPGTGIAPSEISNIVGARLSMNLSAGTTLL
                     WEHIEA"
     gene            complement(6794..7492)
                     /gene="neuA"
                     /locus_tag="U6309_001093"
     CDS             complement(6794..7492)
                     /gene="neuA"
                     /locus_tag="U6309_001093"
                     /EC_number="2.7.7.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N,N'-diacetyllegionaminic acid synthase"
                     /protein_id="HEN5504546.1"
                     /translation="MRILAVIPARAGSKRLPGKNTRLLAGKPLIAHTIVAALQSSCCE
                     EIVVSTDSKQIADVAVQYGASVPWLRSEDLATDTSDVIHTVIDLLFKFQQMEVFFDSV
                     LLLQPTSPFRKPETIRHAVEIHQATGKSVVSVSPISLKPSWCRSIDSQGNLVKPELFQ
                     DLEIYCNENPIYKLNGSIYIATAKQIIENKSFYSEPTKPLLLNSISESIDIDTPIDWA
                     LTEKLMELNQEALV"
     gene            complement(7505..8146)
                     /gene="hisH"
                     /locus_tag="U6309_001094"
     CDS             complement(7505..8146)
                     /gene="hisH"
                     /locus_tag="U6309_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEN5504547.1"
                     /translation="MSSVSIVDYGVGNLLSVARAFQYFDASVNLVSTPEEIMSADRLV
                     LPGVGAFEDGMKGLTTLNFIEPIKQFARSGKPFLGICLGMQMMLSRSTEFGQHEGLDL
                     IAGEVVSVPSHGVDGQLHKIPHIGWNELVSTSEGEDWCHTILKNIPLNSSVYFVHSFM
                     AMPSNPKKRLADTLYDGQAISAVIKDENMYGCQFHPEKSGEVGLNIIQQFLQI"
     gene            complement(8143..8904)
                     /gene="wbuZ"
                     /locus_tag="U6309_001095"
     CDS             complement(8143..8904)
                     /gene="wbuZ"
                     /locus_tag="U6309_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl amidation-associated protein WbuZ"
                     /protein_id="HEN5504548.1"
                     /translation="MSNVRLIARLDIKGPNLIKGVHLEGLRVVGNPNEYAMAYYAQGA
                     DELIYMDTVASLYGRNNLSEIVKTTAENVFIPITVGGGIRSVDDAKQLLRCGADKVAI
                     NTAATKNPTLISDVARRFGSQCVVLSIEAKRTVNGRWEVMTDNGREHTGMDVVDWARN
                     GEEFGAGEILLTSIDQEGTRKGFDLELVKQVSSMVSIPVIASGGMGKLEDLTEVVCES
                     KADAVAMADVLHYKRIGLPEIRKYALDNGIHVRVL"
     gene            complement(8904..10259)
                     /locus_tag="U6309_001096"
     CDS             complement(8904..10259)
                     /locus_tag="U6309_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HEN5504549.1"
                     /translation="MSKIYAPKSIDLSSFQLPDEKLQTKYGLPHKVEFCKSCVISNQR
                     PNSAVEYEHKKESKKHTIHFDDEGICDACRVAERKKSTINWEERDRQLRELCDRFRSK
                     DGSYDCVVPGSGGKDSFYAAHILKYKYGMNPLTVTWAPHMYTPWGWRNFQSWIHAGFD
                     NHLFTPNGRVHRLLTRLAVENLFHPFQPFMIGQKAYAPKMALLHKIKLVVYGENEAEY
                     GNPIGDTESAKRDWKYFTADDKSKIFLGGTSVQELKSDFGLNDNDLDAYLPADPQQIE
                     EQQVEVHYLGYYLKWHPQSCYYYSVEHGGFEASPERTPGTYSKYNSIDDKIDDFHYYT
                     TLTKFGIGRATYDASQEIRSGDITREEGVALVKRFDQEFPERFAEEIFKYLSINPKEF
                     PIASQMFEQPIMDRAYFMALADTFRSPHLWKKEGEQWKLRHQVTNLEKTKAEYLDLET
                     V"
     gene            10446..11264
                     /locus_tag="U6309_001097"
     CDS             10446..11264
                     /locus_tag="U6309_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5504550.1"
                     /translation="MSLKAMYNQIAENYATANRFGSISESHHVAIEQMRKFHLGLKPH
                     YKILDLGVGNGSFLQKLHHLMPMANFTGIDVSSEMLKRASEALPLTTIEGSAAEANKF
                     LPAHSQDLVLAHFINAYIPINVLFDEAKYLTRANGHFSLITTTYDSFPVAQQQLANFI
                     AQDTILSRVVGHYYKSIVKNTTVAANLDELLLAFKQHQFNILDHQRIEIPITLNNIDE
                     LALFGIEGTWFLNTLSIRMLPKYFLIQRLKRLFSKIFTFPYHDTHIIDVVLARK"
     gene            11303..12811
                     /locus_tag="U6309_001098"
     CDS             11303..12811
                     /locus_tag="U6309_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEN5504551.1"
                     /translation="MKLISQAVKNYLPELSLRQKQTNNIIFITFWSQFSVYALNTVLV
                     LFLTRPLIAQGLGYSQAKAYAFIGVTQATGYLMPILGGYMADNIIGVRRSILLGSIML
                     ACAYLLVMLSGYTISSLGDQLFIIAFAFIPATNSLLMGTASSMVSHIYSDDAIKAKSA
                     MTYYYMAINVGALLATMIAPVLLESRYGPLSVLTLTFIGKSIAALNFAKRYSIYDSVI
                     WGMDKSKFSNQGLLRLVAYIAAIYSLTLYAYTHVYIASTLIGAGCAIGILWFLIKTIM
                     LKGETRSKQMIAVLLIIEAIVFFIIYNQMNSTLVLFAENNSDLSFLWFKISPAQYQML
                     NPLLILLIGSQLPRFYRFFSRFTIPYQFAAGTILSGIALLVMAFAAQNAINGLVNGNY
                     IALTYILISIAELWVSAIGLSMIGLYCDARAIAFAMGVWYLASSMSNAISGRIAGWVA
                     IPENINSALESLPYYKNYYLIMGISALGLGILMYFVAYYLHKIMKRRGIELA"
     gene            13211..13492
                     /locus_tag="U6309_001099"
     CDS             13211..13492
                     /locus_tag="U6309_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN5504552.1"
                     /translation="MFWVYILQCSDKSYYTGQTDNLEKRLTQHQDKMIPGCYTSTRLP
                     IQLKFSQEFMSREEALSAERQIKGWSRRKKEALINGDWQALSDYSKRKN"
     gene            complement(13646..14635)
                     /gene="lipA"
                     /locus_tag="U6309_001100"
     CDS             complement(13646..14635)
                     /gene="lipA"
                     /locus_tag="U6309_001100"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEN5504553.1"
                     /translation="MGKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPK
                     WLRIVNHTTPAYSQVKEQVQKHRLATVCEEAKCPNISECWSHGTATIMLMGAVCTRAC
                     RFCSVDTGNPHGWLDAEEPENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRA
                     IKKRSPRTKVEALTPDFQGSERDVAVLLDSGVDVFAQNVETVERLTHPVRDNRAGYQQ
                     TLNVLAFAKKYRPDVLTKTSLMLGLGETDEEIIQTMDDLRTHHVDILTLGQYLQPTKN
                     HLPIARYVTPETFSELRQIGLKKGFFEVASGPLVRSSYRADRVFKRDNLGLDV"
     gene            complement(14787..16337)
                     /locus_tag="U6309_001101"
     CDS             complement(14787..16337)
                     /locus_tag="U6309_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEN5504554.1"
                     /translation="MIDQELKKKIVNTISQYEHKCDKMQNQINTLRNVINQLIINPIG
                     INYELDDRLSQLRNYLDTEFEPAVIEKKVKRLAEILSKMQRKKAENSRLVNGLIKQGV
                     ESIGRIANKSQDKRAVTKLQKMLDTEMESHAILIRFNQVLTQCISSVIKELEELPQIT
                     KVDNPSKSYQSEISLKVNDSLQQLLNHLSIPQDLDSKRGIIKTNLEHALTDEELSTII
                     DNLTELVVESFNVEQNRFKGFLQQLTDQLNDFEIYLRVSGDARKKASEDSRLLECGIQ
                     DNLDQIKNHLDTSKTIEELSSKVSQNIKMIGDRLKEYRHNEKIREEEYAKQVSELQAR
                     LQESEQNAEEIKNLLAFQKYRINHDSLTGLPNRESYEEHILDSFQRWKRSSSALSLAV
                     CDIDHFKRINDNFGHLAGDKVLKKVAMIFKSSIRTVDFIARIGGEEFVFIFEQTSSKE
                     AFGILEKLRKLVEDCLFYYRDKKVDVTVSFGLTTVTEKDDIESLFMRADNAMYKAKNA
                     GRNRVEIL"
     gene            complement(16357..16629)
                     /locus_tag="U6309_001102"
     CDS             complement(16357..16629)
                     /locus_tag="U6309_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEN5504555.1"
                     /translation="MDDNNSEFKHLFPIAVEVTEGKTYVWCGCGKSKTQPFCDKENCG
                     DKAVSFIADLTEDIYFCNCKQTKSPPFCDGSHAKILLEVVKKRQNK"
     gene            complement(16741..17928)
                     /locus_tag="U6309_001103"
     CDS             complement(16741..17928)
                     /locus_tag="U6309_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504556.1"
                     /translation="MKTMKQMIRLTMLLLMLSFNLWAINSSSPWYTEEKGKGLVINVE
                     LYLSSTCPHCHKADEFFKEIEKHYTWLHVERYIIDKDKKALKRFGDLLTELNRYDFAV
                     PSIFFCNSRWVGFATAQTTGKDLLNGIEYCKEEIVRNGRLTPATESVLNRWGNANLFD
                     SNITGSPSTNTFIFVVALIDAISPCALFTIAAFLGVLFMIEKRKLQFISGSVFILTVA
                     GVHYFQQVYPTLFFESLSWFRIPAALVGLFAFYFAGQYYKKHSIQPLFIVLAFLLALT
                     VPMFQQTCLMNWSYVFEQWLHNQNVSGVQMGLYQFAYQLIYIFPLIILMFLYWILMKI
                     HFFKKFKTKLTTIGFLYILAIALLLIIYPYALSNLALSLFLIVSLGISGLLLNWFNAS
                     KMT"
     gene            complement(18044..18478)
                     /locus_tag="U6309_001104"
     CDS             complement(18044..18478)
                     /locus_tag="U6309_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN5504557.1"
                     /translation="MAHLIHSVLPVPPRKIAYIHPDDSVKECIKQMVEKDIGALVVFD
                     NDAHLIGIVSERDILRCYFHKNLSLETAKVSDVVYNNVTILSPHDSVEKAMQVITETK
                     RRHVLIQEEGELLAILSIGDLLYHVLEDKLRVIEHLENYIHS"
     gene            complement(18784..19233)
                     /locus_tag="U6309_001105"
     CDS             complement(18784..19233)
                     /locus_tag="U6309_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEN5504558.1"
                     /translation="MKKLIVASLSLTFMLGGCASMNKEGVGTITGGVVGGLLGSQFGG
                     GSGKVAAAGAGALLGAYLGGQIGKTMDKVDRLEMQRALETAPTGKAVAWSNPDTGYRY
                     TVQPTRTYYNAQQPCREYITKAMIGGKTQQIYGKACRQADGSWRVVN"
     gene            complement(19306..20379)
                     /gene="alr"
                     /locus_tag="U6309_001106"
     CDS             complement(19306..20379)
                     /gene="alr"
                     /locus_tag="U6309_001106"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEN5504559.1"
                     /translation="MSRPTRLVIEPSALLHNLSQIKHLAPGKKVIAMVKANAYGCGVR
                     EVAPVLDGRIEAFGVACLEEALAIRALGVETPCILFQGVFSSDELSVAVENDFACILH
                     HAQQLEWLIKTPLPYPIKIWVKVNTGMHRLGFKIHELQKVMGALQTCTWVDKNIGLMT
                     HLACADEPHRPENQQQISLFQEISIPGFRQRSIANSAAIISFPDSHADVVRPGIMLYG
                     VSPFANQNARDLSLIPVMRFMSAISAIHDNPSFAQVGYGGTWKSDKPSRIGIVAAGYG
                     DGYPRHISEKTPVWVRGREVSIVGRVSMDMLTIDLTEHPDVEIGDEVELWGTHVLVER
                     IAKSAGTVGYELLCQISERVRYK"
     gene            complement(20381..21763)
                     /gene="dnaB"
                     /locus_tag="U6309_001107"
     CDS             complement(20381..21763)
                     /gene="dnaB"
                     /locus_tag="U6309_001107"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEN5504560.1"
                     /translation="MLDLQISKKTVDPLKRPPHSVEAEQSIIGGLMLDNQVWDKVSNK
                     LCETDFYRTEHRILFRAISALAKKDQPFDVVTLLDALKTRNELDDAGGEAYLFELANN
                     TPSVANVTAYADIVREKSVQRQLIAVATEIADSAYNPAGREVPELLDMAETKVFAIGE
                     QTASDGGPENIKSILVRAVEKIDALYHNGDAITGLATGLSDLDEMTSGLQPSDLIIVA
                     GRPSMGKTTLVMNMAEHAAIKAGKPVLVFSMEMPADSLAMRMMSSLGRIDQHRIRTGK
                     LDDNDWPRVTSAVHMLSEAPLFIDDTPALSPGEMRARARRLAKEHGSLGLIVVDYLQL
                     MKVPGFNADNRTAEISEISRSLKSLAKELQVPVIALSQLNRSLEQRADKRPVMSDLRE
                     SGAIEQDADLICFIYRDEVYNEDSPDKGTAEIIVAKQRNGPIGKVRVAFLGKYTRFED
                     LAFNGYQGVE"
     gene            complement(21948..22382)
                     /locus_tag="U6309_001108"
     CDS             complement(21948..22382)
                     /locus_tag="U6309_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEN5504561.1"
                     /translation="MKLWKVLCSFVIAVCYLPLAFAASQSPAGTWTTIDDKTGAKRAV
                     VNLKVSNGTLSGTIVKVYPQPGDTGICSKCPGAFKDKKIQGLTFVWGLKDEGNGVWSG
                     GSILDPKTGKIYKAKMTLQGNKLYVRGYFGVSALGRTQTWIR"
BASE COUNT         6758 a         4836 c         3815 g         7080 t
ORIGIN      
        1 tatttgataa tttatgaaca aaatcacctt gtggaaccca acccaattcc ttttgaattt
       61 tactattgtc tatggcgtat cgtttgtcat gaccttttct atcttcaaca aaagtaatca
      121 atgaatgata tggtttttcc ataggtttta aatcatccat catctgacaa atcattttta
      181 tcaaacttaa attatccagt tcgttattac cacctatatt ataaacttcc cctaatgctc
      241 ctttttgaat aatggtatca attgcttcgc aatgatccat gacatacaac caatcgcgaa
      301 tatttgatcc gttgccataa acggtgatgg gcagctgatt gacacaagcg aatactacct
      361 taggaatcaa cttctcttta tgttgattag gcccgtaatt attcgaacaa ttcgaagtgg
      421 taacaggtaa tccataggta tgataataag ctctgactat atgatcagaa cttgccttgg
      481 acgctgaata aggggaattg ggttgatatg aatttttttc agtgaacgcc ggctcttctc
      541 tttctaatga accatacact tcatcagtgg atacatgatg aaaccgacac tttgatttat
      601 cccattgcct ttcattcaac caataaatcc ttgccgcctc taacagagta aaagttccaa
      661 ttacattggt ttcaagaaat atttgcggat tatcaatgga attatccaca tgggattcgg
      721 ctgcaaaatg gacaagcgta tctatttcat attcccttag taaggataac accagacttt
      781 tgtccaatat gtctccttgc acaaaaaggt gatttttaca ttctgccatt tcactcaaat
      841 tggctttatt tcctgcatag gttaatttat ccaaactgat tattttgatc tccgggtatt
      901 tatcgttcat aaatttaaca aaattacttc ctataaagcc cgcagctcca gttaccaaaa
      961 ggttgttcat ctgatttttc atagtgagat tcttactttt ttgtaaaatg gtcgataaca
     1021 tctggcaaat catttttcca actacagctt tttataccca atattgtttc tatctttgtt
     1081 gtattgagta ccgaattttt aggccttgtt gccctagtag gatattcttg tgacttaatt
     1141 ggatttaaat cggttaaagt caagagaagc tgtttctctt ttgccatatt aagaaatacg
     1201 tttgcaaatt catgccaatt ggtaacaccc tgccctgcat aatggtaaag cccccaatag
     1261 tcaaaggaag aatgtctgat tttctgtact atttcaaata gtactcttgc tatatcgcga
     1321 gctgaagtag gccttcccga ttgatctgaa acaatattca attcttttct gctggaagcc
     1381 aaatttaaaa tcgtttttac aaaatttttc ccttgttcac caaataccca gctaactcgc
     1441 aaaataatat actttttaag ttgggataaa atagcctgtt ctccttccca tttcgtgcgc
     1501 ccataggtac ttactggatg cggaatatcg gtttcatgat aggctccctc cttttctcca
     1561 tcaaatacat aatcagttga aagatggatc aaggggacgt ttttcaaggt acaataattc
     1621 accaggcgac agacaaattc ggcgttcaca atatgagcca aatcaggttc atcctctgcc
     1681 ttatctacag ccgtataggc tgctgcatta atgataagat ccggcttgat ttccgataac
     1741 acatcatgaa cccgttcgag tttggaacaa tctagaatat ctctggtaca agcatatact
     1801 tcatgctcag aggaagaaaa tcgcttgata atctcggtac caacttgccc attagcacct
     1861 gtaaccaata ttttcattta ggcttgatat ctcggtaaaa gttcagcatc tattaaattt
     1921 aaaagcttgg ctgcagcatc ttttggagac aattgaacct gctctcctaa tggccaatca
     1981 attgctaatt gcggatcaag ataatgaatg gaaaactcac tttcaggatg ataatagtca
     2041 gtacatttat aagcaaaatc agccatagca ctcaaaacat aaaacccatg ggcaaatcct
     2101 ttaggaatcc aaaattgcct tttgttttct ccggaaagaa tcactccaac ccattgccca
     2161 aaagtagggg agcctaacct aatatccacg gcaacatcaa acacttcacc agctaaaact
     2221 gaaaccagtt ttccctgagt ttgttgactt tgataatgca aacctcttag tacacctttt
     2281 tgtgatcttg aaaaattatc ctgtacaaaa cgatcagtta ttcctaataa ctcttcatat
     2341 cgtttagctt gaaaagattc ataaaaaaac cccctttcat caccataaat ctttggttcg
     2401 ataattttaa gttcatcgat ctgagtattt attatattca tatcgttact aaagctctta
     2461 aaagagaatt ataattcctt aatatttaaa tcttttacta cctgtatcca gttttcacta
     2521 gaacaaataa ttaagctacc tcaaaattgt tttgtttata tttaataaaa atatcattca
     2581 aacaatgaat gactctatca atgtctctgg gggttaaatt tgacccggaa ggaagacaaa
     2641 taccatgatc aaacaagtaa tctgaaacgc tgaattgttc atcatgagga tagtatttag
     2701 agcctgcaaa taaaggctgt ctatgcatag gcttccatgt tcttcttgct tcaatgttaa
     2761 atggctttaa ttgctcaatg acttcttccg gttttatctt gtttggcgtt ggtttaataa
     2821 ccaatgtcga taaccaacga gtactgtatc cttgggttat ctctggcatc atgtcaataa
     2881 atgggtaaga ttccaaggct tgcttatatc gttcaaaaat agctcgtctt gccttaaccc
     2941 gtttttccaa tactttcagc tgacctctgc ctataccagc aagcacatta ctcattctgt
     3001 aattataccc aaccacagta tgttcataat ggggcattgg ttcgcgagcc tgagttgata
     3061 aaaatcttgc tctttgtatg agactctcat cttcagacac caacatgccg ccaccagacg
     3121 tggtaatgat tttgttgcca ttaaatgaaa acacgcctaa cttgccgaaa gtcccgctgt
     3181 acttgttatt ataggtggct cccaaagact ccgccgcatc ttctataatt ggcacattgt
     3241 attcattgca taattgacat aaagcgtcat aattagcact ttgtccatat aaattaacaa
     3301 taatcacagc ttttggcatt tgattttttg ctttactttc tcttaatgcc cgctcaagag
     3361 caactggaga catattccag gtgtccaaat cagaatcaat aaataccgga gtcgcacctt
     3421 ggtataagat aggattcact gtggctacaa aagtaaagct ggatgcgaac acaatatctc
     3481 ctggtttcac atcgagtaat acgagtgcca gatgaattgc agctgttcct gagcttaaag
     3541 ccacagctga ttttatatta atgtattccg ccacttcttt ttcaaaagaa tcaacattgg
     3601 ggcctaaagg ggctacccag ttggtggcaa aagcttcctg tacgtattgc tgctcatgct
     3661 caccgatatg aggcgaggaa agcagaatat tggaagagag ttctctccgg tggatcaagc
     3721 gtatggggcg attactctca tcgataacgg gaatatgatc taattcatat tcctgcatca
     3781 aatgataagc atcctgaatc gtagcagaga caggtaaagc ttttgatgaa tgctctgata
     3841 attcctctaa agtagaatct aatgtaaatc cttttaatag caatcttcgg atgtcaccat
     3901 ctgtaactgt ccttattaaa tgttcatcac tatctaccag aaataacaca ccttgagctg
     3961 tagcatccag cttgctcaaa gcagttttca gattttcttg tttttgtata tataactctt
     4021 tgatagtaat cattttattc cttttaaact gcgggtacgc ctttcacaac agcattttct
     4081 tttacatcct tcacaacgac tgagccagct ccaattattg ctccatcatc tatagtcaca
     4141 ccaggcaaaa caactgctcc agctccaatc agcactctct caccaatctt gactcgccca
     4201 cctaaggtgc tattaggcgc aatatgtgaa cacgagccaa ctatcacttc atgatccaca
     4261 acggcactgt gattgatgat acatccctca cccacctgac attcagggcc gagtattgcc
     4321 tgtgctgcaa taaaggagcc tgcaccgagc ctcgctgatt tcgcaataat ggcagccgga
     4381 tgaataattg taaacaagtt tgcattggaa ttcatcaaat ctataacatt ctttctggcc
     4441 tgattatttc caatgctgac gtgcacataa ccaatatagt cagctaaact gtccatagtt
     4501 gaatcaacca atactccaca cacctcctga cccagcaaac tcttattgct atcacaaagt
     4561 gaaacttgta aagagtgctg agtcagtgat aaagcatcaa tcaccacctt acagtgtcca
     4621 ccacatccta ttattttcaa gttagtatgc attgcattta ttcaatattt gtgagtgcaa
     4681 agggatagtt tttaacaatt gataacacct ttcactcgtt tttccatccc cgtaacaatt
     4741 agagtatttt atcttgggtt tatttagcgc ttctcttaga ccagtcaaaa tagcatcata
     4801 agtaacatca acatcaatga cattgtcgct tcgttctctt aaattttgcc tgcttccaac
     4861 attaactacg ttcaggttaa atgaggctgc ctctatgatg ccactactgg aatttcccag
     4921 catcacatca gaatttgcaa gacaatcaat aaattccgga cgatgtaggt gcttgataat
     4981 tctaacatca ggatgatcaa tatattcctg aatcacttct cgaattaaat gaccacccgt
     5041 atctgaattc ggctcaaggc aaataatctg caaatttgta gcgagtgctg cctgaatcac
     5101 gctttgaaat tgaattttaa tcgagtcaac ttcttgaaca accgggtgat agattaataa
     5161 acaaattttt ttgtcaggat gaaatccata acgttgatta aacacatcac gtgtagacgt
     5221 tttatactgc atgatttcat ccaagcctgg agcaccaacc tgaaaaatgg tttcttcctt
     5281 ctcacccatt ctaatcaatc gttgtttgga tgcctctgtt gcgacaaaat gataatgaga
     5341 taatttggaa atcgcatgcc ttaccatttc atcaacggtt ccagagcgct ctcctccgtg
     5401 cagatgtaca accgggatat ttaaatgtat cgctgctata gctgcggcca acatttcacc
     5461 tcgatctccc agcaataaaa cgacatcagg agtttcactt tcgaatacct cggtgaatcc
     5521 caaaagctca tggccgatag cttttgccat agaactatgt tgggcattgt caagatcaac
     5581 aggaataatg ccgcatattg agaactgatc tgctttaatt tcgttaactg tatttccata
     5641 caaagcatca agatgcatac cagtgacaca aatcgataag tcaatgtctt ctgactggtg
     5701 taatcttttt agtacttctc tcatcagtcc ataatcggca cgagtacctg taacataaat
     5761 tatttttctg gtcattacgc ttcaatatgt tcccataaca aagtagtacc tgcagacaaa
     5821 ttcatactta atcgagcccc tacaatattt gatatttctg aaggcgctat cccggtaccg
     5881 ggtcttagca agatcaaatc ttccttactg atttgtgctc ctttcaccaa atcacgtctt
     5941 aaagtaatac ttcttctaac cagagctctt atcggcagct cattatccga cggttttttt
     6001 tcaccacttc ccaaaacagt ttcagcgtct ctgatggact gcactaaatt cttcatttcc
     6061 tctggattca tagaagcaag atgatctgga ccaggaagag atttatccat tgtaaagtgc
     6121 ttttcgataa cgcacgcccc cattcctact gctaatgttg gcaccaaaat acctaaagta
     6181 tgatcagaat aacctactgg caattgaaat tcgtcagcta atgtctgcat ggctttcaaa
     6241 ttcacgtcct gataagctgc cggataatta gaggtacaat gcaacaaaac cagataatcg
     6301 gataaagagt ttccatagta gggttttact gtatctatgg ctactcttac ttcttgtaga
     6361 tcgcacatcc ctgttgagat gattaatggt aacttcttac tggcacaatg ctgcaagtaa
     6421 ggtacattag taatttcgcc agaagggatt tttaatcgct ttacccctaa agaaataaga
     6481 aaatcaatgc tttgttcatc aaaaccagtt gacataaatt caatgccgag tgaattggct
     6541 aattcactta gtaaataatg gtcctcttct gaaagctcca gtgccttgag catctcgtac
     6601 tgggtagagg attccggggc attatttttt tggtattccg ctttttctac tgttttattg
     6661 accaaggtgt ccgctttgaa agtctgaaac tttactgcat cagctcctga ctctttggct
     6721 gcataaacta attctttagc cagttgaaga tcaccattat ggtttactcc tgcttcagca
     6781 ataataaaac aagtcatact agagcctctt ggtttaattc cattaatttt tctgttagag
     6841 cccaatcgat tggcgtatcg atgtcgatgg attcactaat actatttaac aataaaggtt
     6901 tggttggttc gctataaaaa ctcttatttt cgataatttg tttagctgtt gcaatataaa
     6961 ttgacccatt taatttataa attggattct cgttgcaata aatttccaga tcctgaaaaa
     7021 gctctggctt aaccaaattg ccttggctat caatgcttct acaccaagaa ggctttaaag
     7081 agataggact aactgaaacg acactttttc ccgttgcttg atgtatttca acagcatgtc
     7141 ttatggtttc tggcttcctg aatggagaag ttggttgtaa aagcaataca ctgtcaaaaa
     7201 aaacctccat ttgctgaaac ttaaacagga ggtcaataac agtatgaatc acatccgaag
     7261 tatccgtggc taaatcttcc gatcttagcc aaggtactga agccccatat tgaacggcga
     7321 cgtctgctat ttgtttacta tcggtcgaaa caacgatttc ttcacaacaa gacgactgca
     7381 aggcagcaac aatagtatgt gcaattaatg gctttccggc aagtaatctg gtatttttac
     7441 cgggcagcct ctttgagcca gcccttgccg ggattactgc caatattctc atttttaatg
     7501 caccctaaat ctgcaaaaac tgttgaatga tgtttaaacc tacttccccg cttttttcag
     7561 gatgaaattg gcatccgtac atattttcat cttttattac tgcgctaata gcctgaccat
     7621 catataaagt atcagccaga cgctttttag gattactagg catggccata aaagaatgaa
     7681 caaaatatac tgaagaattt aatggtatat ttttcaggat agtatgacac caatcctcgc
     7741 cttccgaggt ggaaaccagt tcattccagc ctatatgagg tattttatgc aattgaccat
     7801 caactccatg tgaaggaacg ctaacaacct cgccagctat tagatccagg ccttcatgtt
     7861 gtccgaattc agtacttctt gagagcatca tctgcatgcc aagacatatt cccagaaaag
     7921 gttttccact ccgcgcaaat tgctttatgg gttctataaa atttaatgta gttaatcctt
     7981 tcatcccatc ttcaaaagcg cctactcctg gcaaaaccaa ccgatcggca ctcataattt
     8041 cctcgggtgt gctcactaaa ttgacagaag catcaaaata ttgaaaagct cgagccacac
     8101 ttaataaatt gcctacccca taatctacaa ttgaaactga actcataaga ctctcacatg
     8161 aatgccatta tctaaagcat actttctgat ttcaggtaaa cctattcttt tataatgcaa
     8221 aacatccgcc atagcgactg catcagcctt agactcacaa accacttcag ttaaatcttc
     8281 cagcttaccc atacccccac ttgcaatcac cgggatagaa accatggaag acacctgttt
     8341 cactaattcc agatcaaatc ccttacgcgt accttcctga tcaatagagg tcagaagaat
     8401 ttcacctgca ccaaattctt cgccattcct agcccaatca acaacatcca ttccggtatg
     8461 ttcacgtcca ttgtctgtca tgacctccca tcttccattc acagtgcgtt ttgcttcgat
     8521 agataaaaca acacattgag acccaaatct tctggcaaca tcagatatta gagtagggtt
     8581 tttagttgcc gctgtattaa tagctacctt atcggcaccg caacgcaata attgcttggc
     8641 atcatcaaca ctgcgaattc cgccacctac agtaattggg ataaatacat tttcagcagt
     8701 agtctttaca atctctgaca aattattacg cccatataaa gatgcaactg tatccatata
     8761 aatcaattca tctgcacctt gtgcataata tgccatagca tattcattgg gatttcctac
     8821 tactcttaat ccctccagat gcactccttt gattaggtta ggtcctttaa tatctagtcg
     8881 tgcaattaga cggacattac tcatcaaaca gtctccaaat ccagatattc agctttagtt
     8941 ttttcaagat ttgttacttg atgtctaagt ttccactgtt ctccctcttt tttccacaag
     9001 tgaggagatc tgaaggtatc cgccaaagcc ataaaatagg ctctgtccat aatcggttgc
     9061 tcgaacattt gcgaagcaat gggaaactct ttggggttaa tactcaagta tttaaaaatt
     9121 tcttcagcaa aacgctctgg aaactcttga tcaaaacgct tcaccagagc aacaccttct
     9181 tcgcgagtaa tatcccccga acgtatttcc tgggaggcat cgtatgttgc tcttccaata
     9241 ccgaatttgg ttagcgtggt gtagtaatgg aaatcatcaa ttttgtcatc aatactattg
     9301 tatttactgt aggttcctgg agttcgttca ggagaagctt caaaaccgcc atgttccaca
     9361 gaataataat aacaactttg aggatgccac ttcagataat aacccaagta atgaacctct
     9421 acctgctgct cttcgatttg ctgtggatca gcaggtaggt aagcatccaa atcattgtca
     9481 tttaaaccga aatcgctttt taactcttga acagatgtac cacctaaaaa gattttgctt
     9541 ttatcgtccg cggtaaagta tttccagtct cttttcgcag attccgtatc acctattgga
     9601 ttaccatatt ccgcttcatt ttctccataa acaaccaatt taattttatg caacaaagcc
     9661 attttaggag cataagcttt ttggccaatc ataaagggtt gaaatggatg gaatagattc
     9721 tcaactgcaa gtcgtgtcaa tagcctgtga actctgccat ttggagtaaa cagatgatta
     9781 tcaaatccgg catgtatcca agattgaaaa ttcctccaac cccatggcgt atacatatgg
     9841 ggagcccaag tcacggtcaa gggattcatt ccgtatttat attttaaaat gtgggcagca
     9901 taaaaactat cctttcctcc ggaaccagga acaacgcaat cataagaacc atccttgctt
     9961 ctaaatcgat cacaaagctc tctcaattgc ctgtctcgct cttcccaatt aatagtagat
    10021 tttttgcgtt cggcaactcg acaggcatca caaatgcctt cgtcatcaaa atggatggta
    10081 tgtttttttg attctttctt gtgctcatac tctacagcag aattaggcct ttgatttgaa
    10141 ataacacagc ttttgcaaaa ttccacctta tgtggtagac catactttgt ttgtagcttt
    10201 tcatctggga gctgaaaact tgataaatca atggactttg gtgcgtaaat tttgctcata
    10261 tcaattccta tcctggcatt agacattttt aaaaaaccgc agtatacaat aaaaatttca
    10321 ggtattaaac aaatcctgat tgaagaacca aaatatataa tttaataaat ttgtgttttc
    10381 aatagtcgcc aattaccccc ttttagggat atactggatg aatcgagtta aagtaggaat
    10441 tttgcatgtc cttgaaggcc atgtataacc aaatcgcaga aaattacgcg acagctaatc
    10501 gttttggttc aattagtgaa agccatcacg ttgccattga gcagatgagg aaatttcatt
    10561 tggggttgaa accccattat aaaatcctcg atctgggcgt tggcaatggc tcttttttgc
    10621 aaaaattgca tcacttaatg ccaatggcca atttcacagg aattgatgtt tcatcagaaa
    10681 tgttgaaacg agcgagcgaa gcccttcctt taacaaccat tgaaggcagt gctgcagaag
    10741 ccaacaagtt tttgcccgca catagccagg acttggttct ggctcatttt attaatgcat
    10801 atatccctat taatgttctc tttgatgaag ccaaatactt gacgcgtgcc aacggtcact
    10861 tctctttaat cacaacaacc tatgattctt ttcctgtagc gcaacaacaa ctggccaact
    10921 tcattgctca ggatactatt ttaagccgag ttgttggtca ctactataaa tccatagtta
    10981 aaaatacgac tgttgctgca aatctcgacg aactcttgct ggcatttaaa cagcatcaat
    11041 ttaatatttt ggatcaccaa cgtattgaaa tacccattac ccttaataac attgatgaat
    11101 tggcactgtt tggaatagag ggcacctggt ttcttaacac tctatcgata cgcatgctgc
    11161 caaaatattt tttaattcag cgtcttaaac gacttttcag caaaatcttt acttttccct
    11221 accatgacac tcatattatt gatgtagttc ttgccaggaa gtgataatct ttggcggtgg
    11281 gttagaaaaa acggaatgag gtgtgaaatt gatttctcaa gcagttaaaa attatcttcc
    11341 agaactaagt cttcgccaaa aacaaaccaa taatattatt tttattactt tttggagcca
    11401 gttttctgtc tatgccctga atacagttct ggttttattt ttaactcgac ccttgattgc
    11461 tcagggatta ggctacagcc aagccaaagc ctacgctttc attggtgtaa ctcaagccac
    11521 tggctacctg atgcctattt taggcgggta tatggccgat aatattattg gagtcagacg
    11581 atccattctt ttgggtagca tcatgctggc ttgcgcttat ttactggtta tgttaagcgg
    11641 gtacaccatc agttctcttg gggatcaatt atttatcata gcttttgcct ttattcctgc
    11701 aaccaactcg ctattaatgg gaaccgcatc cagtatggtt tctcatattt attccgatga
    11761 tgccattaaa gccaaatccg ctatgactta ttactatatg gctattaatg ttggggctct
    11821 gctggccacc atgatcgcgc cagttctcct ggaaagtcgt tatggtccac tttcagtact
    11881 caccttaacc tttattggca aatcgattgc tgccttgaat tttgcgaaac gttactccat
    11941 atatgatagt gtcatctggg gtatggataa atccaaattt tctaaccaag gtctattgcg
    12001 ccttgttgct tatatagcgg ctatctattc tcttacttta tatgcttata ctcatgttta
    12061 tatcgccagc acactgatag gcgcaggatg tgctatagga attctctggt ttttaattaa
    12121 aaccattatg cttaaaggcg aaactcgcag taaacaaatg atcgctgtat tactgataat
    12181 agaagccata gtatttttta tcatatacaa tcaaatgaac agtaccttgg tattatttgc
    12241 agaaaacaat tctgatctaa gttttctttg gtttaaaatt tcacccgctc aataccaaat
    12301 gctaaatcct ttgctcattt tactgattgg aagtcaactt ccccgtttct acaggttttt
    12361 ttcgcgtttt acgatcccct atcaatttgc cgcaggcaca attctctctg gtatcgccct
    12421 gctggttatg gcttttgcag cccaaaacgc aatcaatgga ttagtgaatg gtaactacat
    12481 tgctttgacc tacattttaa tttcaattgc cgaattatgg gtcagtgcca ttggtcttag
    12541 catgattgga ctgtattgtg atgcacgtgc gattgccttt gcgatgggtg tatggtacct
    12601 ggccagttcc atgtccaatg ccatttcagg acgaattgca ggttgggtcg ccataccaga
    12661 gaatattaat tctgctcttg aaagtttacc ctattataaa aattattatt tgattatggg
    12721 cattagtgct ttagggctcg gtattctgat gtattttgta gcttattatt tacacaaaat
    12781 catgaaaaga cgaggtattg agttagcata acgtagctga acattgtaat attcgaatat
    12841 gcttcgagac ggtgcttcgc acctcctcag catgaacggc ctgatgggaa aatccaattt
    12901 tgtaatcagc cttggagttt tatgcaaacc atcttccgat tgtccataaa tttttcttgt
    12961 taattggata aatcagttat tatattaatc cgttcatgct gaggaagtca gtgcaccatc
    13021 ttgaagcaag aatggcccga tgggaaaatc caattttgta atcagccttt gggctttatg
    13081 caaaccatct tccgattacc cataaatttt aatcggataa attagttatt atcttaatcc
    13141 gttcatgctg aggaggtgcg aaacaccgtc tcgaagcatg tttaaaaaca tagtataagg
    13201 gattgactaa atgttttggg tttatattct tcaatgcagt gacaagagtt attatacagg
    13261 gcaaacggat aatctggaaa aacgccttac tcaacatcaa gataaaatga ttccgggttg
    13321 ttatacaagc acaagattac caattcaatt aaaattcagc caggaattta tgtctcgtga
    13381 agaagccttg agcgctgaga gacaaatcaa aggatggagt cggagaaaga aagaggcttt
    13441 aataaatgga gactggcaag cgttatcaga ctattcaaaa cgtaaaaatt aagagtgggc
    13501 tgtgtttcat tcgaacatgc ttcgagacgg tgcttcgcac ctcctcagca tgaacggttt
    13561 aatagtttaa taaaaaatct aatttcttta attaataata aaattattta tagccaactc
    13621 gaaaaacaca agctgttcac taacttcaaa catcaagccc taaattatct cgtttgaaca
    13681 cccggtcagc cctatagctt gatcgaacta atgggcctga agctacctca aaaaagccct
    13741 tttttagacc aatttgcctt aattcagaaa aagtttcagg agttacgtaa cgtgctatag
    13801 gtaaatgatt tttagttggt tgcaaatatt gtcccaaagt caaaatatcc acgtgatgag
    13861 ttcggaggtc atccatagtc tgtataattt cctcatcggt ttcacccaat cccaacatca
    13921 aactggtttt ggtcagaaca tccggtcgat atttcttggc aaaagccagt acattcaagg
    13981 tttgctgata acccgctcga ttatctctta ccgggtgtgt caggcgctca accgtttcta
    14041 cattttgcgc aaacacatca acgccactat ctaacaatac agcaacatct ctttcacttc
    14101 cctgaaaatc gggggttagc gcttcaactt tggttcgggg agatcgtttt ttgattgctc
    14161 taatggtttt ggcataatga ttcgcccccc catcaggtaa atcatcacgg ttaactgagg
    14221 ttaaaaccac ataatccaga ttcattaagg ccacagtttc tgcggtattc tcaggttctt
    14281 ctgcatcaag ccaaccatgc ggatttcctg tatccacaga acaaaatcgg caagcccggg
    14341 tacatactgc tcccattaac ataatagttg cagttccatg agaccaacat tctgagatat
    14401 ttgggcattt cgcttcttca caaactgttg ccaaacgatg tttttgcact tgctctttaa
    14461 cctggctata ggctggagtt gtatgattga ctatacggag ccacttcggt tttggcaatc
    14521 gttcatgatc ttgacctgat gattttattc catctttaat agctgtcaca ccttgagatg
    14581 tcttatattt ttgtccactt tccacaacga ttggaatatc gatcaatttg cccatgacgt
    14641 catccgaaat acaaagagtt atgatgatcc caaatcataa agataagatc aagttcattt
    14701 aaaaatcgtt atcagaggaa aatcatctgt taccatggat ttactccgaa aatagtataa
    14761 ttaaaaattg ttggaaatat attccactat aatatttcta ccctgttcct tcctgcattt
    14821 ttagctttat acatggcatt gtctgcccgc ataaataaag attcaatatc atctttttcg
    14881 gttaccgtag ttagcccaaa agaaaccgta acatcgactt ttttgtctcg ataataaaac
    14941 aaacaatctt caactaattt acgtaatttc tccaaaattc caaaagcttc cttgctgcta
    15001 gtttgttcaa agataaacac aaattcctca ccacctattc gagcgataaa atcaaccgtc
    15061 ctgatcgatg atttaaaaat catagctacc tttttaagaa ccttatctcc tgcaagatgg
    15121 ccaaaattat cattaatacg tttaaaatga tcgatgtcac acacagcaag agacaatgcg
    15181 cttgaacttc gtttccagcg ctgaaatgag tccagaatat gctcctcata agattctcta
    15241 ttaggtaaac ctgttaaact atcgtggtta attctgtatt tctgaaaagc aagcaaattt
    15301 tttatttctt ccgcattttg ctcggattcc tgtaatctag cttgcaactc ggatacttgc
    15361 tttgcatatt cttcctctcg gattttttca ttgtgtcgat attcttttaa cctgtcacca
    15421 atcattttta tattttggct cacctttgaa gataattcct caatcgtttt agaggtatcc
    15481 aaatgattct tgatttgatc aagattatcc tgaattccac actctaataa acgactgtct
    15541 tcacttgcct ttttgcgagc atctcctgat actcttaaat agatttcaaa atcattgagc
    15601 tgatcagtta actgttgaag aaaccctttg aatcgattct gctcaacgtt aaatgactca
    15661 acgactaatt ccgtcaaatt gtcaataata gtgctaagct cttcatcggt gagtgcatgt
    15721 tccaaatttg tttttatgat tcccctcttg gaatccaagt cctgagggat agataagtgg
    15781 ttaagtaatt gttgcaagct gtcatttact tttaaactaa tctcagattg ataacttttg
    15841 gaggggttat cgacttttgt aatttgtggt aactcctcca gctccttaat cacagaagaa
    15901 atacattgag ttaagacttg attaaaacga atcaaaatcg catggctttc catctcggta
    15961 tccagcattt tttgtaattt tgtaacggct ctcttatcct gagatttatt ggcaatacga
    16021 ccaatggatt ccacgccctg tttgattagt ccattgacta aacgagaatt ttctgctttt
    16081 ttcctttgca ttttggaaag tatttctgcc aaacgtttca cttttttttc tatgacagca
    16141 ggttcaaatt cagtatccaa ataatttctt agttgcgaaa ggcgatcatc caactcataa
    16201 ttaattccaa tgggattaat aatcaattga ttaatcacat ttctgagcgt attaatttga
    16261 ttttgcatct tgtcacattt gtgctcatat tggctaattg tgttaacaat ttttttcttt
    16321 aattcttgat caatcattta agacttcctt tcccgttcat ttattttgcc tttttttaac
    16381 cacttcaagc aaaatctttg catgcgatcc atcacaaaaa ggagggcttt tggtttgttt
    16441 gcaattgcaa aaataaatgt cttcagtcaa atctgctata aaagagactg ctttatcccc
    16501 gcaattctct ttatcacaga aaggctgggt cttgcttttg ccacaaccac accatacgta
    16561 agtctttcct tctgttacct ccacagctat tggaaataaa tgcttaaact ctgaattatt
    16621 atcatccatg atgagaattt ctccataagc gatcagcttc taagcctggc tcatttttaa
    16681 ttgtatgcca taaaatgaaa gatattcaga atttataatt attttatgcc gattgaaccc
    16741 tcatgtcatt ttagaagcat taaaccaatt cagtaacagg ccggagatac ccaatgaaac
    16801 aattaaaaat aatgataaag ccagattaga caaggcatag ggataaatga ttaataagag
    16861 tgcgatagct aaaatatata aaaaacctat agtagttaat tttgtcttaa attttttgaa
    16921 aaaatgaatt ttcattaaaa tccaatatag aaacatcagg ataattaaag gaaagatata
    16981 gataagctga taagcaaatt gatatagccc catctgaact cctgatacat tttggttatg
    17041 taaccattgt tcaaacacat aagaccaatt catcaaacag gtttgttgaa acattggcac
    17101 tgtaagtgcc aataaaaaag ccaaaacaat aaataatggt tggatggaat gttttttata
    17161 atattggcca gcgaaataaa aagcaaaaag gcctaccaat gcagccggaa tacgaaacca
    17221 tgaaagcgac tcaaaaaaca gggtaggata aacctgttga aaataatgaa cacctgcaac
    17281 agttaaaata aacacggaac cgctaataaa ttgcagcttt cgcttttcaa tcatgaataa
    17341 aacacctaaa aatgcagcaa tcgtaaataa agcacatgga ctgattgcat cgattaaggc
    17401 tacaacaaaa ataaatgtat ttgtggaagg gcttcctgtg atattggaat caaacaaatt
    17461 cgcattcccc catctgttta aaacactctc ggtcgcagga gtcaatcttc catttctcac
    17521 aatttcttct ttacaatatt ctatgccgtt taacagatcc tttccggttg tctgtgcagt
    17581 ggcaaaccca acccaacggg aattacaaaa aaatatggat ggaacggcaa aatcatatcg
    17641 attaagttct gtcaataaat cgccaaagcg tttcaaagcc tttttgtctt tatcaataat
    17701 ataacgctca acatgcagcc aggtataatg tttttcaatt tctttgaaaa attcatcggc
    17761 cttatgacaa tgaggacaag tagatgaaag atacaattca acgttaatta caagcccttt
    17821 acctttttct tctgtatacc agggagatga agaatttatc gcccataaat taaaactgag
    17881 catcagaagt aacattgtca atcgaatcat ttgcttcatg gttttcatta tatttctttg
    17941 taaatgaata ctatagcact taggatcgat tgtatgaaat agccaaaaca agactctcgc
    18001 gtttaaagca ataaaatgta aatctgaaat aaaagcttat atttcaggag tgaatataat
    18061 tttccaaatg ctcaatcact cgcaatttat cttccagtac gtggtacaac aagtctccta
    18121 ttgacagtat agctaataac tcaccttctt cctgtattaa tacatgtctt cttttcgttt
    18181 cagttatcac ttgcatggct ttctctaccg aatcatgtgg acttaagatc gtgacgttgt
    18241 tataaacaac atcagatact tttgcagttt ccaaacttag attcttatga aaataacagc
    18301 gaagtatgtc tctttcactc acaataccaa tcaagtgggc atcattgtcg aaaacaacta
    18361 atgcacctat atctttttca accatctgtt taatacattc tttgacacta tcatcaggat
    18421 gaatataagc gattttcctt ggtggcaccg gcagtacgct atgaattaag tgagccattg
    18481 cactctccct gattaatact aaattagcaa ctcgctgtat aaatatagca caaaacaagg
    18541 aaggttaagc tcattgtgtg agggcaggaa agatttttat aaaagattat cgtaggattt
    18601 gtatattgaa aactccaagg tcaaggcaaa aaatgatttt aatgaaggaa tttagataaa
    18661 ctaaatgact gaattaaaaa tatttttaac aaagagatag gagtttttcg taagtcctgt
    18721 cttaattaat ttgagtaata aaatgagatt gttgggtttt ccccaacaat cttaatcaat
    18781 taattaattc acaacacgcc aggaaccatc agcttggcga caggccttac cataaatttg
    18841 ttgggtttta ccaccaatca tggctttggt aatatactca cggcaaggtt gttgagcatt
    18901 ataataagtt ctcgttggct ggacagtata acggtatcca gtatccggat ttgaccatgc
    18961 aactgcttta cctgttggag ctgtttcgag ggccctttgc atttcaagtc ggtctacttt
    19021 atccattgtc tttccaattt gaccacccag gtaagctcct aataatgctc ctgctcctgc
    19081 agctgcaacc ttacctgaac caccaccaaa ctggctacca agtaatccac ctaccacacc
    19141 acctgtaatt gtacctacac cttctttgtt cattgaggca cagccaccta gcatgaatgt
    19201 tagggataat gatgcaacaa ttaatttttt cattacagtt cacctgttta aagagttaca
    19261 aaatcggttg tcattctacc atcacgaaca gttattatcc ataacttatt tgtaacgtac
    19321 tctctccgaa atttgacata ataattcgta tcctactgta cctgctgatt tggcaatgcg
    19381 ctctactaaa acatgtgttc cccaaagctc aacttcatcg ccgatctcaa catcagggtg
    19441 ctctgtcaaa tcgatggtca gcatatccat agaaactctg ccaacaatag acacttccct
    19501 ccctctaacc caaaccggtg ttttctcgga aatatgccgt gggtacccgt cgccataacc
    19561 tgctgcaaca atgccaattc gcgaaggttt atcgcttttc caggttccac catagcctac
    19621 ctgagcaaaa gatgggttat catgaatcgc gctgatggct gacataaaac gcatgacagg
    19681 gattaaactt aaatcacggg cattttgatt ggcaaatggg gaaacaccat acaacataat
    19741 cccgggcctc accacatcag catgggaatc agggaatgaa ataattgccg ccgaattagc
    19801 gatactgcgt tggcgaaatc caggtatgga tatctcctga aacaaagaga tctgttgttg
    19861 attctcagga cgatgaggct catctgcgca agccaaatga gtcattaacc ctatattttt
    19921 atcaacccag gtacaggttt gcaatgcccc catcactttt tgcaattcat ggattttaaa
    19981 tcccagtctg tgcatgcctg tgtttacttt cacccatatt tttatcgggt aaggcaacgg
    20041 agtcttgatt agccattcca actgctgtgc gtgatgaaga atacaggcga aatcattctc
    20101 aacggctact gacaattcat cagaactaaa gacgccctga aagagaatgc acggggtttc
    20161 aacacctaaa gctcgaatag ccagagcctc ttcaaggcag gctactccaa aagcctcaat
    20221 acgcccatca aggacagggg caacttccct gactccacaa ccataagcat tggcttttac
    20281 catagcgata acttttttgc ctggagctaa atgcttgatt tgagataaat tatgcaataa
    20341 agcactgggc tctatcacta aacgtgtagg tcttgacacc ctattctacc ccttgataac
    20401 cattaaatgc caaatcttca aatcgtgtgt acttacccaa aaaggccacc ctgactttgc
    20461 ctatgggtcc atttctttgc ttggcaacaa tgatttccgc agtgccttta tcaggactgt
    20521 cctcattata tacttcatct ctataaataa aacagattaa gtcagcatcc tgttcaattg
    20581 ctcctgattc tcgcaaatct gacatcactg gccgcttatc tgctcgctgc tcgaggcttc
    20641 tattcagctg agataaagca attacgggaa cctgtaattc ttttgccagc gatttaagac
    20701 tccgagaaat ttcggagatt tccgctgttc tattgtctgc attaaacccg ggcactttca
    20761 ttaattgtaa ataatcgaca acgataagcc ctaatgaacc atgttccttt gctaaacgtc
    20821 tggcacgggc cctcatttct ccaggactta aagctggagt atcatctata aacaaaggag
    20881 cttcagaaag catatgcacc gcggaagtca ctcgtggcca gtcattatca tctaatttac
    20941 cagtacgaat ccgatgctga tctattcgcc ccaaggaaga catcattctc attgccaggg
    21001 aatcagcagg catttccata gagaaaacca atactggctt accggcttta attgccgcat
    21061 gctctgccat attcatcacc aaagttgttt ttcccatcga tggacggcca gcaacgataa
    21121 tcagatcaga aggctgtaaa cccgatgtca tttcatccaa atcggataag cctgtcgcaa
    21181 ggcctgtaat tgcatcacca ttatgataga gggcatctat tttctctacc gcccgtacca
    21241 aaatggattt aatattttct ggacccccgt cactcgctgt ttgttcacca atagcaaata
    21301 ctttggtttc agccatatcc aatagctcag gcacttctct tccagcagga ttataagcgg
    21361 aatcagcaat ttctgttgct accgctatca gttgcctctg gactgatttt tcccgaacaa
    21421 tatccgcata agcggtcaca ttcgctacac ttggtgtgtt attagccaac tcaaataaat
    21481 aggcttctcc accagcatca tctaactcgt tgcgtgtttt taaagcatca agaagcgtca
    21541 ccacatcaaa aggttgatct tttttggcta aagcagaaat ggctctaaaa agaattcggt
    21601 gctcagtacg ataaaaatcg gtttcgcata atttattgct taccttatcc cagacttgat
    21661 tatcgagcat caaaccacca ataatggatt gctcagcctc tacagaatga ggggggcgtt
    21721 ttagtggatc aaccgttttt ttagatattt gcaaatcaag catggtaata atattttttg
    21781 agaatttatt tatagggaag acaatttatt taaagacatt cctaatctaa ttgcaaatga
    21841 tcataacact aaaaacaaaa tgcgggatag taccaactgc tttcaatgac aagagaaagc
    21901 agttggtact atcccacatt atatataaaa acatccttca gtactactta tcgaatccag
    21961 gtttgagtac gtcctagagc agaaactcca aaataccctc tgacatacag tttattaccc
    22021 tgcagtgtca tcttagcctt ataaattttt cctgttttgg gatcgaggat agagccacca
    22081 ctccagacac cgttgccttc atcttttaat ccccaaacaa aagtcaaccc ttgaattttt
    22141 ttgtccttga atgcgccagg gcacttagaa caaattccgg tatcacctgg ttgtggatat
    22201 accttcacaa tagtaccact taaagtacca tttgatactt taaggttcac tactgctctc
    22261 ttagctccgg ttttatcatc aatggttgtc caggtaccgg caggagactg tgatgctgca
    22321 aaggctaatg gtaaataaca tacagcaatt acaaaactac ataacacttt ccataatttc
    22381 ataagtaatc tccgtaattg aatcagtata cctcttgtat aaattctgta ctcttttgga
    22441 atacaaagtt caagttatac aagagcttgt aataaagcat aagccatga
//
LOCUS       DASLOE010000036        21629 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.036, whole genome shotgun sequence.
ACCESSION   DASLOE010000036 DASLOE010000000
VERSION     DASLOE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 21629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21629
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.036"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(90..1433)
                     /gene="nadA"
                     /locus_tag="U6309_001109"
     CDS             complement(90..1433)
                     /gene="nadA"
                     /locus_tag="U6309_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEN5504562.1"
                     /translation="MFKNSTTYQPEATLMAIRNDMAICQSDYPLDWYQEDFIPYAQEY
                     QALPDRKLTTVLNWMKPYLTKAQNHFGNKLLLLAHYYMGGEIVRLIEQFGGKIGDSYQ
                     LALMAANNPEKSVIVESAVHFMAESISILANENQHVYITNPKSGCTMEMLAKDFMVEP
                     AFLDLNERYGAENILPVCYMNTSGRVKAMTGAQGGAVCTSSNVKKIFQWAQKQNKKIL
                     FIPDQHMGENVAYWLGIKNLAYWPGGTAGANYSLPAQDKKTLKQFDKAELILFASQCA
                     VHTHYQPEMCDYWKSLGYTTIVHPECRNDVIKVADYSGSTAFIWDLVVHDKAGTKSYA
                     IGTENHMVENLKQYCKNLGIRVVNLAEAPQKDTEKGVGCGCATMSRNDPPHLVALLDL
                     LRQGKSMPYNEVKAGDVVNEFTGTRNRLPVNDQQWVINNAKKALEMMINITEDRV"
     gene            complement(1508..3157)
                     /gene="nadB"
                     /locus_tag="U6309_001110"
     CDS             complement(1508..3157)
                     /gene="nadB"
                     /locus_tag="U6309_001110"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEN5504563.1"
                     /translation="MSDQLSQQGQSFEFDVLVIGTGLAGLHYCLQLLAIQPKLNIALI
                     SKAESIECNSRYAQGGIAAAISPEDSLESHISDTVLAGDGLCYLPAVEFIIRQGPDII
                     KQLDQYKVQFKRENDGRFHLAKEGGHSHRRIFNCGDQTGLTLTQTLNHLARQHQQIQF
                     FEHHVAVNLITQYHPHRTDNQGEVLGAYILDCQHNRIHTFLAKCVILATGGAGKTYRY
                     TTNPMVATGDGVAMAYRAGARVGNMEFYQFHPTLLHHHTINNFLISEAVRGEGALLKN
                     PETGERFMKRYAPDQLELATRDVVARAIFSEIEQGQAGYVHLDISHQSKSFLKKRFPQ
                     IYSTLLSIGIDMSQDLIPVVPAAHYQCGGVLTDVDGRTDLARLYAIGEVAFTGLHGAN
                     RLASNSLLEALVMGSNAARCTLKDIATPLKLIDHIPSWSSPGEVNARRASQINAQWRG
                     LRGEMTSYAGIVRTEAGLEDLLQLILKRKKIIEEYYWKHCITRDFIELRNIILNAELI
                     VRAALSRRESRGGHFREDFPNKNFKAQESIAKLGQADSSLG"
     gene            complement(3154..4524)
                     /gene="purB"
                     /locus_tag="U6309_001111"
     CDS             complement(3154..4524)
                     /gene="purB"
                     /locus_tag="U6309_001111"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEN5504564.1"
                     /translation="MTLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWF
                     ESLAANDTIPEVPALDNKARKFLSDLISNFNESEAEKIKEFEKQTNHDVKAVEYYLQD
                     KFQENEQLKSCVAFIHFACTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQ
                     HADVAMLSRTHGQPATPTTMGKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHV
                     AAYPEVDWRKHCANFVTSLGLSFNAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIW
                     SYISLGYFKQKTIAEEVGSSTMPHKVNPIDFENAEGNLGLSNALFIHFANKLTQSRMQ
                     RDLSDSTVLRNLGVAFSYSLIAYHSVAKGNDKLQINKSALQKDLSENWEVLAEAIQTV
                     MRRYNEPNAYEQLKELTRGQMIDAENLKKFIKTLSIPEEAKAELMKLTPETYTGLATQ
                     LVKAFS"
     gene            4677..6380
                     /gene="sulP"
                     /locus_tag="U6309_001112"
     CDS             4677..6380
                     /gene="sulP"
                     /locus_tag="U6309_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEN5504565.1"
                     /translation="MNTITESYRAGLFQKKYWLQNIISGLIVGVVALPLAMAFAIASG
                     AKPEQGLYTAIVAGLIVSIMGGSRVQIAGPTGAFIVVLSGITAQYGISGLQIATLMAG
                     FILVLFGFARLGSIIKFIPSPVIIGFTSGIAVVIWVGQWQCFFGLPAAGSGHFHEKFW
                     HLLLSFPHMNIATTFLGICALFIVLYSSKLPGLKRIPGPLIALVLVTIIQSVWHFEGV
                     ATIGSLFGGIPQGLPAFTWPEITMGRLIELIGPAFAIAMLGAIESLLSAVVADGMSGT
                     RHNSNRELVGQGIANIVAPLFGGFAATGAIARTATNIRNGGNSPLSGIIHSITLILVL
                     LFLAPLAVDVPLAALAAILFVVSWNMSEARHFIKLIRVAPRADVVILLVTFCLTVFVD
                     LVVAVNVGVIIAVLHFLRRMVNSIQIQRMSEGQLSQELAQEKNYDLPEGVMVYVIEGP
                     FFFGAVETFQSALANTHTDPKVLIIRMRWVPFIDVTGLQAMEEIILNLQKRGVRVLLS
                     EANPLVDAKLRKMGIVQLIGEQNYYKEFSQALDVCRINDSDRKSQPPFEVAKPLASVT
                     Q"
     gene            complement(6583..8286)
                     /locus_tag="U6309_001113"
     CDS             complement(6583..8286)
                     /locus_tag="U6309_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEN5504566.1"
                     /translation="MSIKNEYETARDHFRLWDKQLKDNILLEKSCLMHTYKTYFPDEP
                     GFIQQLSQFSKAVAEELEPTVMENNLDANLPKIEQYNAIGKRDDRVIHHPAYVRSGDI
                     IYGSNLMQYLLKPGQMKKTLSLFLLSSHAGEAGHNCPIAGSAGVIRVLSRYSQLEKTS
                     YYLDKLTHPSFSYNFTGAQFLTEIQGGSDVGANATRANLDKQNQWRISGEKWFCSNAN
                     ADLILMTARFDEETPGTKGLGLFLVPSRLDDGSPNHYYLRRLKQKIGTRTMATAEIDF
                     EGALAYPMGDVAEGIHLVMENVLHLSRIFNSFSVLGMSRRAYQIACYYAKNRRAFNHP
                     IIEYPLVKESLAKIKSENLAMLASVFHMARCQDELDALPEPQQTKSQQLLLRTLANIN
                     KYFTAKRSVDNIHHCLDLLAGNGTIENFSSLPRLLRDCIVCENWEGTHFTLWMQTLRD
                     MHKFAIDELFIAHLQQLLEQVQDINPHKALFFEKINELDTTIKTMKSANHEEQSLHIK
                     TIIEHMASLLAALCLAIEIQQGQSPETKQAGLTLFIHDHIASYLLQHDDYMKILNKVI
                     G"
     gene            complement(8515..9513)
                     /locus_tag="U6309_001114"
     CDS             complement(8515..9513)
                     /locus_tag="U6309_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEN5504567.1"
                     /translation="MFKRIGLISIALSLNTISLTSEACSALVFNKNKPATVAVNLDWK
                     YREGAFVIHPRNAMMVSSVDYHSMHPLIWKSQYGSVIFHGGNRFKAGPSADGMNEKGL
                     TASILMLDSSSYPSNSELPALKTTEWVQYILDNFQTVQEVIDDSSNYQLVADTYRGIT
                     MNVHLMVSDAEGKSAVFEYLDGKLVIHTQDNLTTPVLTNTDYKSSLALLEEYQDHGGS
                     KALPGGYDSKSRFVRAAHYLKKLPSFIAKEEHIAYAFNGLNVVTQAPGTSSPTQLSMV
                     FDIPTKTIYFRSINEAALRVIPMDSIDFDYLYQPIALNVYQHLSGDVTDKFQPMIG"
     gene            9738..12125
                     /gene="ppsA"
                     /locus_tag="U6309_001115"
     CDS             9738..12125
                     /gene="ppsA"
                     /locus_tag="U6309_001115"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEN5504568.1"
                     /translation="MTVKRHTIDLAHLGMRDLDQVGGKNSSLGEMISHLSSAGVSVPG
                     GFATTADSFREFLSQNGLDKKIYDKLTALDTDDVCQLAVVGKQIREMIIDTPFTPEFE
                     QSVRSSYQKLAQEIGHDNFSVAVRSSATAEDLPDASFAGQQETFLNVKGEEAVLAAIK
                     QVFASLFNDRAIAYRVHHNFAHNEVALSAGIQQMIRSDLAVSGVMFTMDTESGFDQVV
                     FITSSYGLGEMVVQGAVNPDEFYVHKPCLEAGKPAIIRRNLGSKALKMVYCDDPSLER
                     RVKTVDVDSAERLLFSLSKEEVEQLANQALIIEKHYGRPMDIEWAKDGVNGKLYILQA
                     RPETVKSRDNKQVLERYTLQKRGDVLAEGRSIGQRIGQGKAKVIKDINEMHRVQPGDV
                     LISDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATKTIKDGDV
                     VTVSCAEGDTGFVYSGLLPYEQERLDVETMPELPMKVMLNVGNPERAFAFQSIPNSGV
                     GLARLEFLISNTIGIHPKALLEFDTLKDEELKRYIKEKTIAYDSPVEYYIERLKEGIA
                     TIAAAFYPKPVIVRLSDFKSNEYANLVGGSLYEPQEENPMLGFRGASRYVSSNFSECF
                     ALECKAVRRVREEMGLDNVEVMIPFVRTVSEAGNVIEVLKKHGLERGKCGLRVIMMCE
                     LPSNALLASEFLQYFDGFSIGSNDLTQLTLGLDRDSGLVASQFDERNDAVKALLHMAI
                     AACKKEGKYIGICGQGPSDHQDFAQWLMKEGIESVSLNPDSVLQTCLFLAKQSMN"
     gene            12367..13806
                     /locus_tag="U6309_001116"
     CDS             12367..13806
                     /locus_tag="U6309_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN5504569.1"
                     /translation="MSNSKWWFLLVCFIPAITFASEGGEHSDPVTSVLLGVTTILFLA
                     IIGRYLARLLNQPGVLGELLIGMIVGNLSYFFGSELVVILREGSAIYDVVKEMLAGIS
                     LNQAVTHVIHNSYYANQVIHALSGAEGTEYLKIAYVVDIFSRYGVIFLLFMVGLETSL
                     SDLKKTGKESILVAVIGIVAPMILGFVVACFIIPNSNYKINLFIGATLSATSIGITAS
                     VLKEMNKLSTREARTILGAATLDDILGLIILAVVSSIVISGAVSIMVVMKIIILALLF
                     FIGTLFMGPFVLRKAVDLFRFLEPWESKLFISFLFIMALAWLASFIQLAAIIGAFAAG
                     VILHDDYFGEQKAHHKDHRSIHHLVSPLETILAPMFFTLIGIQVKLETFYDWNVIVLA
                     GGLSVAAILGKLLSGLGANRRDDRLLIGIGMLPRGEVGLVFASIGRTIGVISDNLFTA
                     IVLMIMITTFIAPPLLKMRYAKPKEDRNA"
     gene            13803..14645
                     /gene="nadC"
                     /locus_tag="U6309_001117"
     CDS             13803..14645
                     /gene="nadC"
                     /locus_tag="U6309_001117"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEN5504570.1"
                     /translation="MNNVLSQVTFDVKRALQEDVGNGDVTAALLPQQLIVEAEIISRE
                     PMLVCGQPWVNEVFKQVDNTVEMEWRVSEGDFLSTPATLCIIHGTASSILTAERTALN
                     FLQTLSATATQTYHYVQKLKGTKAKLLDTRKTIPGLRLAQKYAVHCGGGLNHRMGLYD
                     AFLIKENHIKACGSVAHAISLARKTDKHLLVEIEVETLEELQEALDAHPDRILLDNFT
                     QDMLEQAVKMNHPKYCELEASGGININNIADIAQTGVDFISVGSITKSIQAIDLSLLI
                     RETL"
     gene            14642..15733
                     /locus_tag="U6309_001118"
     CDS             14642..15733
                     /locus_tag="U6309_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEN5504571.1"
                     /translation="MSPSIVFTGGGTAGHVTPNIALIKEFRKEGWNVEYIGSVSGIEK
                     EMIEPMDIPFHGVSSGKLRRYFSLKNLLDPFKIVLGIIQSSLLFYKIKPDVVFSKGGF
                     VAFPVVVGAWLNRIPVVAHESDMSPGLANRLSFPFVNKICLTFDAGKKYFKRQDKIEV
                     TGTPIRQQLLTGNRMKGLELCGFNSSKPCLLVVGGSLGAGSINSCIRSALKQLTSEFQ
                     VIHLCGKGKLDSSLVGVEGYCQFEYANEELADLFAASSVVISRAGANSLYEILALGKP
                     HILIPISSQVSRGDQIQNARYFQGLGISVVIQDELLKADVLLQAVQDVMRKKDEIDNK
                     IKALKIESATDKIVAIIKEQAHVQTPRIV"
     gene            15708..17123
                     /locus_tag="U6309_001119"
     CDS             15708..17123
                     /locus_tag="U6309_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEN5504572.1"
                     /translation="MFKPQGLYDYICQQWQEEILPSLCDYIKIPNKSPHFDAKWEEHG
                     YMEQAVNHIANWCKSHAPKGMTLEIVRLKNRTPLLFMEIPGQIDDTVLLYGHLDKQPE
                     MSGWSDDLHPWKPVLKNGLLYGRGGADDGYSAYASLTAIRALEQQGLPYPRCILIIEA
                     CEESGSYDLPFYIELLKERIGKPSLVICLDSGAGNYEQLWMTTSLRGNLVGKLTVELI
                     NEGVHSGSASGIVADSFRVARQLISRIEDENTGEIKLPQLYCDIPDERIKQAKQCAEI
                     LGEQVYSEFPWIDSAKPVIQDKQQLILNRTWRPALTVTGADGFPAIADAGNVMRPVTS
                     LKLSMRLPPLVDPEAASVAMEKALTQNPPYNAKVDFKIQNGGSKGWNAPLLSDWLAKA
                     ASEASMTYYDKPAAYMGEGGTIPFMSMLGEQFPKAQFMITGVLGPHSNAHGPNEFLHL
                     DMVKKLTSCVSYVLYSFSQKK"
     gene            17215..17532
                     /locus_tag="U6309_001120"
     CDS             17215..17532
                     /locus_tag="U6309_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEN5504573.1"
                     /translation="MKARLIAILLSFIVIPFSSQAENEPQKTPAKQVLVKGKINLNTA
                     DVHSLTGSFKGIGKKRAEAIIAYRENHQGFKSLEELAEVKGIGQRFVDKNREKLKEIF
                     VIN"
     gene            17728..18765
                     /locus_tag="U6309_001121"
     CDS             17728..18765
                     /locus_tag="U6309_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEN5504574.1"
                     /translation="MFRKLRGVFSSDLSIDLGTANTLIYVRDKGIVLNEPSVVALRNE
                     SGQKRVAAVGLEAKRMLGRTPGNINAIRPMKDGVIADFFVTEKMLQHFIHKVHENKFL
                     RPSPRVLVCVPCGSTQVERRAIRESAMGAGAREVFLIEEPMAAALGSGMPVEEASGSM
                     VVDIGGGTTEVAIISLSGIVYHQSVRIGGDKFDDAIVSYVRRNYGTLIGETTAERIKH
                     EIGSAFPSRDLFEIEVRGRNLAEGVPRSFTLTSAEILEALQEPLSGIVGAVRAALELA
                     PPELAADIAERGMVLTGGGALLKNIDTLLMEETGLPVLIAEDPLTCVARGGGKALETM
                     DLRGGDFLSTE"
     gene            18743..19651
                     /gene="mreC"
                     /locus_tag="U6309_001122"
     CDS             18743..19651
                     /gene="mreC"
                     /locus_tag="U6309_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEN5504575.1"
                     /translation="MISSQQNNKHNRLFAKGGHSSLGFVFALAFSILLMFSDYHYRYL
                     DFVRSGFSLIVSPLQYAVDYPVRVIGWIQSLVSAKKALIDENMRLKYKQTMLEAELQK
                     LIVIQRENSQLKELLLTSSKADMRVMAAQILAVDTSQARQVVVLNKGTRDGVYVGQPV
                     LDAKGVMGQVIDVGPMTSTLLLISDSKSAVPVRNNRTGERAILVGTNDIEELSLINLP
                     KTSSIHPGDVLVTSGLGRRYPEGYPVGRVEEVKSIPGEDFVKVTVSPVALLNRNRLVL
                     LIWADSEQEELTAQINERLNAEESTA"
     gene            19648..20127
                     /gene="mreD"
                     /locus_tag="U6309_001123"
     CDS             19648..20127
                     /gene="mreD"
                     /locus_tag="U6309_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEN5504576.1"
                     /translation="MSVLHIRLGLGFLVALALSILPMPELISIFRPPWVLLLVLYIEY
                     YLPGKFKLTALLIAGLLLDVLLSTVIGEHSFALLLVTWIASTKSRRVQFFSLIQQVCL
                     IGFFCFLYQSIIIFIDALLGFNYNLIMPVASAILGMIIWPWIRVLGYSSLLTRSVYR"
     gene            complement(20063..20665)
                     /locus_tag="U6309_001124"
     CDS             complement(20063..20665)
                     /locus_tag="U6309_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEN5504577.1"
                     /translation="MPQLILFNKPYGVLTQFTGELPEQTLSAFIKFPGFYAAGRLDKQ
                     SEGLLVLTDDGKLQHRLTHPKFEKKKHYWVQVEGAPTDKDLQPLRKGLVIKDITFLPA
                     EAKLISEPKIWPRIPPIRERKHIPTTWLEIILREGKNHQIRKMTAAIGFPTLRLIRHQ
                     IGDWRLDQLQPGEYKLINLTNDKLNELTRKSNPRLGSRAK"
     gene            20926..21624
                     /locus_tag="U6309_001125"
     CDS             20926..21624
                     /locus_tag="U6309_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEN5504578.1"
                     /translation="MKWVLVVMAIIAQTSCAVNHRVMVGDEPVIIQQTKGQGKTFVHV
                     HHNEQTALKAAKAVIRKEGGSLITLIHSGGRNIVFHLHNQRYEFDPNRIFTDKGIKKT
                     LTQYSYYTPEAHQEVKKLADKIKALLPEGKVIAVHNNSSYSLKDYLPGHELAKDAKAL
                     YMNPDNYFRNFYLVTKLSDYLRLKMQGFNGVLQKPSATDDGSLSVYLAKRDYINVEAG
                     YDQLAEQIKMLLHA"
BASE COUNT         6040 a         3948 c         4778 g         6863 t
ORIGIN      
        1 tacggtcgcg gctcggattt gataagcact gttgatgcaa acccgagccg cgaccataag
       61 gaagcggaca acacactagc taacacgtat taaaccctgt cttcagtgat attaatcatc
      121 atttccaggg cttttttggc attattaata acccattgct gatcgttgac tggcagtctg
      181 tttctggttc ctgtgaattc gttgaccaca tcacctgctt ttacttcatt gtaaggcata
      241 gatttgccct ggcgaagtaa atcgaggaga gcgaccaaat gtggcggatc attgcgagac
      301 atggtagcac aaccacatcc gactcctttt tcagtgtctt tttgaggggc ttcagcaaga
      361 tttacaacac gaattccaag gtttttacaa tattgcttta agttttctac catgtggttt
      421 tcagttccga tggcataact ttttgtacct gctttgtcat ggactaccag atcccatatg
      481 aatgcagtag aacctgagta atcagccact ttgatgacat cattacggca ctctggatgt
      541 acaatggtag tgtatcccag gcttttccaa taatcacaca tttcaggttg ataatgggta
      601 tgaaccgcac attgacttgc gaataaaatc aactctgctt tatcaaattg cttgagtgtt
      661 tttttgtctt gcgctggtag tgaataatta gcgccggcag ttcctccagg ccaataagcg
      721 agatttttaa ttcctaacca gtaagcgaca ttttccccca tgtgttgatc aggaataaac
      781 aggatttttt tgttttgctt ttgagcccat tgaaaaattt ttttgacatt ggaacttgta
      841 caaaccgcac ctccctgggc accggtcatg gctttcacac gaccagaagt attcatgtag
      901 caaacaggta aaatattttc agcaccataa cgctcattta aatcaaggaa agcaggctcg
      961 accataaagt ctttggcaag catttccatg gtgcagcctg acttgggatt ggtgatgtag
     1021 acgtgttgat tttcattagc caaaatgctt atggattccg ccataaaatg aactgctgat
     1081 tccactatga ctgatttttc agggttattc gcagccatca aagccaattg gtaggagtca
     1141 ccgattttgc ccccgaattg ctctattaat cgaacaattt ctccacccat ataataatgg
     1201 gcaagaagta acagtttgtt accgaaatga ttttgggctt tagtaagata aggtttcatc
     1261 caatttaaaa cagtcgtaag ctttctatcg ggtaaagcct gatattcctg ggcataagga
     1321 ataaaatctt cttgatacca atctaatgga taatcgcttt gacatatagc catatcatta
     1381 cgaatagcca tgagggtcgc ttcgggttgg taagtagttg aatttttaaa cattaaatct
     1441 acctctagtc attagggatt tgtgggaagg gttctttcat tattgttaat gggatagctt
     1501 taccaggcta tcctaatgaa ctgtctgcct gtcccagttt tgcaatactt tcctgggctt
     1561 tgaaattttt attggggaaa tcttcacgaa aatgtccacc gcgtgattcc cttctggaaa
     1621 gggctgctct aacaatcaat tctgcattaa ggataatgtt ccttaactca ataaaatctc
     1681 tggtgatgca atgtttccag taatactctt caataatttt cttacgcttc aatattaatt
     1741 gtagtagatc ttcaaggcct gcttcagtgc gcacaatgcc tgcataggaa gtcatttccc
     1801 ctcttaagcc tctccattgg gcgttgattt gactggctct tcttgcattg acttctcctg
     1861 gagagctcca gctggggata tgatcgataa gttttaacgg agtagcaatg tctttgagtg
     1921 tacagcgtgc cgcattggaa cccatgacca aggcttcaag taaggaatta cttgccagcc
     1981 tgttcgcacc atgcaatcca gtaaaagcca cttcacctat agcgtataat cgcgccagat
     2041 cggtgcggcc gtccacatca gtaagaaccc caccgcattg gtaatgagcg gccggaacga
     2101 caggaatcaa atcctggctc atatcaattc caatagataa caatgtggaa taaatttgtg
     2161 ggaaacgttt tttcaggaaa gatttgcttt gatgagaaat atccagatgc acatatccag
     2221 cctggccttg ctcaatttca ctaaaaatag ctcttgctac gacgtctctg gttgctaact
     2281 caagttggtc aggtgcgtat cgtttcataa aacgctcgcc tgtttcaggg tttttaagca
     2341 aagcgccttc gcctcgaact gcttcggaaa ttaaaaaatt gttaatggtg tgatggtgca
     2401 agagggtagg atgaaattga taaaattcca tgttgcccac acgagcccca gccctgtatg
     2461 ccatcgcgac accatcacct gttgcaacca tagggttagt cgtatacctg taggttttgc
     2521 cggcgccacc ggtcgctaaa atgacgcatt tcgcaagaaa ggtgtgaatg cgattatgtt
     2581 ggcagtcgag tatataggct cctaatacct caccctggtt atctgtccgg tgaggatgat
     2641 attgagtaat caggttaacc gccacgtgat gttcaaaaaa ctggatctgt tgatgttgcc
     2701 ttgccaaatg attgagagtt tgtgttagcg ttaatcccgt ttgatcgcca caattaaaaa
     2761 tgcgccggtg tgaatggcca ccttctttgg caagatgaaa gcgaccatca ttttctcttt
     2821 taaattgaac tttatactga tcaagttgtt ttataatatc tggcccttgg cggataataa
     2881 attccaccgc aggtaagtag catagcccat ctccagccaa tacagtatca gatatatgag
     2941 actccagtga atcttctggc gaaatggcgg cagcaatccc cccttgagca tagcggctgt
     3001 tacactcaat ggactctgct ttgctgatta acgcaatgtt tagcttgggt tggatagcta
     3061 aaagctgcaa acaataatgt aacccggcta atcctgttcc aatgacgaga acatcgaatt
     3121 cgaacgattg accttgttgt gatagctgat cactcatgaa aaagccttga ccaattgggt
     3181 tgcaagacct gtgtaagtct ctggagttaa tttcattaac tctgcttttg cttcttcggg
     3241 aatagacagg gttttaataa attttttaag gttttcagcg tctatcattt gtcctcgtgt
     3301 aagctctttt aattgttcat aggcattggg ttcattgtag cggcgcatga cagtttgtat
     3361 ggcctctgct aaaacttccc agttttcgga taaatctttt tgtagagctg atttattgat
     3421 ttgtaattta tcattacctt tggctactga gtgataggca atcaaactgt aagagaaggc
     3481 tacgcccaag ttacgtaaga cagtcgaatc agacagatct ctttgcatgc gtgattgagt
     3541 taatttattg gcgaaatgaa taaataaagc attagacaga cctaaattgc cttcggcatt
     3601 ttcaaagtct ataggattta ccttgtgcgg catggtcgat gagcctactt cttcggcaat
     3661 tgttttttgc ttaaagtagc caagagaaat atagctccat atgtcttggg tataatccag
     3721 aagaatgtta ttaattctca ccataatctg ggaaacttcc gctatgccat catgtggctc
     3781 aatttgagtg gtataggcgt taaaggataa acctagggat gtaacaaaat tagcgcaatg
     3841 cttacgccag tccacttcgg gataggctgc tacgtgagca ttgtaattgc caacagctcc
     3901 attaaatttg gcagggatta atacttctgc caattgttgc tggggtcgtt taaggcgagc
     3961 tacaaaattg accaattctt tacccatggt ggttggtgtt gctggttgac catgcgttct
     4021 tgagagcatc gcaacgtcag catgttgttt tccaagaagc gtaatgcttc ccatgatctc
     4081 agctatagta ggttgtataa cttgggctat cgcttgtttg atcattaaag cataagccag
     4141 gttattgatg tcctctgaag tgcaggcaaa gtgtatgaaa gcaacgcaag atttgagttg
     4201 ttcattttcc tgaaatttat cctgaagata atactctaca gctttaacgt catgattggt
     4261 ttgtttttca aattctttta ttttttcagc ttctgattca ttaaaattgg aaataagatc
     4321 agataaaaat ttacgagcct tgttatccaa agcaggtact tctggaatag tgtcgttagc
     4381 agctaatgat tcaaaccact tgatttcaac cattaaacga taataggtta atgcaaactc
     4441 actaaaatag ggacttaatg ctctggtttt attgacataa cgaccatcta tgggtgaaat
     4501 cgcgtttaat gcagtaagtg tcatgatagg tagcttattc caatattaaa gcgtatataa
     4561 tattagatgc aggacaagat gtgaattaaa gcgataaata ttctagaaaa ttagcgtaaa
     4621 tttgttcaaa gtgcgtataa taatgaccat gtttttaatg ggacaggtta ttatttatga
     4681 acacgatcac agaatcgtat cgagccggtt tatttcaaaa aaaatattgg ttacaaaata
     4741 ttatttctgg cttaattgtt ggtgttgttg ctttaccttt ggcaatggct tttgcaatag
     4801 cttccggggc taaaccggaa caaggattat ataccgctat cgttgccgga ttaatagtct
     4861 caattatggg tggcagcaga gttcaaatcg ccggtcctac aggtgctttc attgtcgttc
     4921 tttccgggat aacggcccaa tatggtattt cagggttaca aatagctact ttaatggcgg
     4981 gctttatctt ggttctgttt ggctttgccc gtctgggatc cattattaaa tttattccca
     5041 gtccagtaat catcggattt acttcaggga ttgctgtcgt tatttgggta gggcaatggc
     5101 aatgtttttt tggtttaccc gcagctggaa gcggacattt tcatgaaaaa ttttggcatt
     5161 tacttctgtc ttttcctcat atgaatatcg cgactacttt tttaggtatt tgtgctttat
     5221 ttattgtact gtattctagt aaattaccag gactaaagcg tattcctggc cctttgattg
     5281 ctttggtact ggttacaatc atacagtcag tttggcactt tgagggagtg gccactatag
     5341 gcagcctctt tggaggaata ccacaaggct tgccggcttt cacctggcct gaaatcacaa
     5401 tgggacgact gattgagttg attgggccag cctttgctat tgcgatgctg ggagcaattg
     5461 agtccttatt gtctgctgtg gttgccgatg ggatgagtgg aaccagacat aattcgaata
     5521 gagaattagt tggtcaaggg atagctaata ttgtggcgcc tttgtttggt ggttttgctg
     5581 ccaccggagc gattgcccgc acggccacaa acatacgcaa tgggggcaat agtcctctgt
     5641 caggaattat tcacagcatt actttgattt tagtcctgtt gttcctggct cctctggcag
     5701 tggatgtacc tctggcggca ttggctgcta ttttatttgt ggtttcgtgg aatatgagcg
     5761 aggctcgcca ctttattaaa ttaattcgag tagctccgcg tgctgatgta gtcatcttat
     5821 tggttacttt ttgtctgacg gtgtttgttg atttggttgt tgctgtcaat gtaggggtca
     5881 tcattgccgt cttgcatttc ctgcgccgaa tggttaacag cattcaaatt cagcgtatgt
     5941 cagaaggaca attatctcaa gagttggcac aagaaaagaa ttatgatttg cccgaagggg
     6001 tcatggtgta tgttatcgag ggcccgtttt tctttggtgc tgtcgaaaca tttcagagtg
     6061 cgttggctaa tacccatact gatccgaaag ttttgatcat cagaatgcgt tgggttccgt
     6121 ttatagatgt cactggtttg caggctatgg aagaaattat tttgaatttg cagaagcgtg
     6181 gagtcagagt tttgctcagt gaggctaacc cgctcgttga tgcgaaactc cgtaaaatgg
     6241 gaattgttca attgattgga gagcaaaatt attataagga attttctcag gctcttgatg
     6301 tttgccgaat taatgattct gacagaaaga gtcagcctcc ctttgaagtt gcaaagccgc
     6361 tggcttcagt tacccagtga gctaagctgg ttatctgttc aagttgagga agcgcttcct
     6421 caactcgaat ggatagaatt aacgtgataa tcgaaaaact tcaaagattg ccatgcctgt
     6481 ttttatatgt aattcaattt gctaaactta aaatctattt ttttggtttg acactacgaa
     6541 taatttttcc cggcctgctg gttaatgcat ttgcagagtg aactatccaa ttactttatt
     6601 tagtattttc atatagtcgt cgtgttgaag taaataactt gctatatgat catgaataaa
     6661 taaagttaat ccagcttgtt ttgtttctgg cgactggcct tgttgtatct ctatagccag
     6721 acataaagca gcaagcaacg aggccatatg ttcaatgatt gtttttatat gcagactttg
     6781 ctcctcatga ttggctgatt tcattgtttt tattgttgta tctaactcat ttatcttttc
     6841 gaaaaacaat gccttatgag ggttgatatc ctgaacttgt tccagaagtt gttgcaaatg
     6901 ggcaatgaat aattcatcaa ttgcaaactt atgcatatct ctgagggttt gcatccataa
     6961 tgtaaaatgc gtcccctccc aattttcgca aacgatacag tctcttaata gtcttggtaa
     7021 tgaagaaaag ttctctatgg tgccatttcc tgccaataaa tcgagacaat gatgtatatt
     7081 gtccacggaa cgtttggctg taaagtactt gttgatattc gccagtgtac gtaataagag
     7141 ttgttgtgat ttggtttgtt gaggctctgg aagagcatct aattcatcct gacaacgagc
     7201 catatgaaac acgctggcta acatagccag attttctgat tttattttag ccaaactttc
     7261 ttttaccaga ggatactcaa taataggatg gttaaatgct cggcggtttt tagcataata
     7321 gcaagcaatc tgataggcgc gacgagacat acccaagact gagaaggaat tgaatatcct
     7381 tgataaatgc aacacatttt ccataaccaa atgtattcct tccgccacat cacccatagg
     7441 gtaggctagt gccccttcaa aatctatttc tgcggttgcc atggtgcggg taccaatttt
     7501 ttgttttaat cgacgaaggt aatagtgatt agggctgcca tcgtccaggc gactgggcac
     7561 taaaaacaaa cccaatcctt tggttccggg agtttcctcg tcgaaacgag ctgtcattaa
     7621 aattaaatcg gcattagcgt tggagcaaaa ccatttttca cctgaaatgc gccattgatt
     7681 ttgcttatcc agattggcac gggtcgcatt ggcgccaacg tcagaaccac cttgaatttc
     7741 agttaaaaat tgggctccag taaaattgta actgaaagaa gggtgagtga gtttatcgag
     7801 ataatatgag gttttctcta attgggaata acgacttaaa acacgtatta ctcccgcaga
     7861 accggcaata gggcagttgt gtcctgcctc cccggcatgc gatgagagta gaaacaggct
     7921 caacgttttt ttcatttgcc ctggctttag caaatattgc atgagattgg atccataaat
     7981 aatatcgccg gatcgaacat aggcaggatg gtggattacc cgatcatcgc gcttaccgat
     8041 tgcattgtat tgttcgattt ttggcaaatt ggcatccagg ttattttcca taacggttgg
     8101 ttctaattct tcagctactg ctttggaaaa ttgactcaat tgttgaataa aaccaggctc
     8161 atcgggaaaa taagttttgt aagtgtgcat taaacaagac ttttcgagca gtatattgtc
     8221 tttgagttgt ttgtcccata acctgaaatg gtctcttgct gtttcatatt cattttttat
     8281 gctcattacc ccatcctttg gtaattaata gatgcattga tatcacagtt ttttaagttc
     8341 ttggtgtact taaatatttt tcaataaatt gtaatagtgg ttattaatca attatccccc
     8401 tcggcttcgc ttgtgccata tccatgcggc ttcacaatag acctcttgcc taaacttgcc
     8461 tctttcatta aacaaaataa tcaggttctg caagagggcc taatgaattc caatttatcc
     8521 tatcatgggt tgaaatttat cagtgacgtc cccggacaaa tgttgataaa cattcagggc
     8581 gattggttga taaagataat caaagtcaat gctatccatt ggaatcactc ttaatgcagc
     8641 ctcgttaatg ctgcggaagt aaattgtttt ggtcgggata tcaaaaacca tactcagttg
     8701 ggttggagaa gaagtaccag gtgcttgtgt gacgacattg aggccgttga aagcataagc
     8761 aatatgttct tctttagcta taaagcttgg aagttttttt agataatgtg ctgctcttac
     8821 aaaacgggat ttagaatcat atccacctgg cagagcctta gagccaccat ggtcctggta
     8881 ttcttcaaga agagccaatg acgatttata gtccgtattg gttagtactg gcgtggtcaa
     8941 gttgtcttga gtatgaatta ccagtttacc atccaggtac tcaaaaacag cagattttcc
     9001 ttcggcatcg ctcaccatca agtgaacgtt catggttatt ccccggtagg tgtctgcaac
     9061 cagttgataa tttgagctgt catctatgac ttcttggacc gtttgaaaat tatctaaaat
     9121 atactgaacc cattcagtag tttttaaagc gggaagctca gagttactgg gataacttga
     9181 tgaatcaagc atcagtattg aagcagtaag tcctttttca ttcatcccat cagcagatgg
     9241 tcctgcttta aaccgatttc ctccgtgaaa tatcacactt ccatattggc ttttccagat
     9301 taatggatgc atgctgtggt aatctacact ggaaaccatc atggcattcc gaggatgaat
     9361 cacaaaggct ccttcccggt atttccagtc caaattgact gcaacagttg cgggcttgtt
     9421 tttattgaac actaaggcag aacaggcttc gctggttaag gagatagtat ttaaagataa
     9481 ggcaatcgat attagtccta tgcgtttaaa catttcaaat ccttttgctt attatattct
     9541 tggaacaggt ttgcttaccc gttcaaccct aaaacatgga cagcaagata tcttaccgca
     9601 tagtacatat acttgtcatt ttttgtgaaa atgccggtat tttgaccttt ataaaaatga
     9661 aatacagcac aagttctaat tccaataaag taacaattta agtagaatgt ttactatttt
     9721 atgaatttgg ggcggttatg actgttaaaa gacatactat agatttggca catcttggca
     9781 tgcgcgattt ggatcaggtc ggtggtaaga actcttctct tggtgaaatg attagtcatt
     9841 tgtcatcagc aggcgtttct gtgccgggag gatttgctac aactgctgat tctttcagag
     9901 aatttttgtc ccaaaacggt cttgataaga aaatttatga taagttgact gctctcgata
     9961 cggatgatgt ctgccaactg gcagtggtag ggaaacaaat tagggaaatg attattgata
    10021 cgccattcac cccggagttt gaacagtcgg ttcgctcttc ctaccagaaa cttgctcaag
    10081 agattgggca tgataatttt agtgttgcag taagatcttc tgctactgct gaagatttgc
    10141 ctgatgcttc ttttgccggt cagcaggaaa cttttttaaa tgtgaaggga gaagaggcgg
    10201 tattggctgc cattaaacag gtatttgctt ctttatttaa tgacagggca atagcttatc
    10261 gtgtgcatca taattttgct cataacgaag tggcattgtc tgctggcatt cagcaaatga
    10321 ttcgtagtga tttggcagtc agtggtgtca tgtttactat ggatactgaa tccggttttg
    10381 atcaagtagt atttatcact tcctcttatg gtttaggtga aatggttgtt caaggtgctg
    10441 taaatccaga tgaattttat gtccataaac catgccttga agccggaaaa ccggcgatta
    10501 tccgtcgaaa cctgggcagc aaggcgttaa agatggttta ttgcgatgac ccttcccttg
    10561 aaagacgtgt taaaacagtc gatgtagact ctgctgaaag attattgttt tccttatcca
    10621 aagaggaagt agagcaatta gcgaatcagg cacttattat tgaaaaacat tatggtcgtc
    10681 ccatggatat tgaatgggcg aaagacgggg tcaatggtaa actgtatatc ctgcaagctc
    10741 gtccggagac tgtaaaaagc agggataaca aacaagtgct cgaacggtat actttgcaaa
    10801 aaagaggcga cgtattggct gaaggccgta gtataggtca aagaatcggt cagggtaaag
    10861 caaaggttat taaagatatt aatgaaatgc acagggttca acccggcgat gttttgatat
    10921 ccgatatgac cgatcctgat tgggaacctg tcatgaagcg tgcttctgca atcgttacca
    10981 atcgtggagg cagaacttgt catgcagcta ttattgctcg tgaattgggg attcctgcag
    11041 ttgttgggtg tggtgatgca acaaaaacga ttaaagatgg tgatgtggtg actgtaagct
    11101 gtgcggaagg tgatacaggt tttgtctatt ctggtctgtt gccttacgag caagagcgtc
    11161 ttgatgtaga aaccatgccc gaattaccca tgaaagtgat gttgaatgtt ggaaaccctg
    11221 aaagagcctt tgcatttcaa tcaataccta attccggagt gggattggcc cgtcttgaat
    11281 tcttgatttc caacacgata ggtattcatc ctaaagcgct tctggaattt gatactttaa
    11341 aggatgagga acttaagcgg tacattaaag aaaagaccat agcgtatgat tcacctgtag
    11401 aatattacat tgaaagatta aaagaaggca tagcgactat tgctgctgct ttttatccca
    11461 aacccgttat agtcaggctt tcagatttta aatcgaatga atacgctaat ctggttggtg
    11521 gctcattgta tgaaccccaa gaagaaaatc ctatgcttgg ttttcgtggc gcctctcgtt
    11581 atgtttcttc aaatttttct gagtgctttg ctcttgaatg taaagcagta agacgagtca
    11641 gggaggagat gggtttagat aatgttgagg tcatgattcc ttttgtccga acagtttctg
    11701 aagctggtaa tgtcatcgaa gtattaaaaa agcatggtct ggaaagagga aaatgtgggt
    11761 taagagtgat tatgatgtgc gagttacctt ccaatgcctt gttagccagt gaatttttgc
    11821 agtattttga tggtttttcc ataggatcga atgatttaac tcaattgact ttaggattag
    11881 acagggattc aggtttggtg gcttcacaat ttgatgagcg caacgatgct gtgaaagctt
    11941 tattgcatat ggctattgca gcttgtaaaa aagaaggcaa gtatataggt atttgcggtc
    12001 aaggtccttc agatcaccag gattttgccc aatggctgat gaaggaagga attgagagtg
    12061 tttctctaaa ccctgactct gttttacaaa catgtttgtt tttagctaaa caatcgatga
    12121 attgatgaac cagagtgtac tggcaattca gtttgaaatt gttaggctgt tggtgcatta
    12181 ggaatttact gaggcattcc tatgattttg aaatgcagca gattgccttg gaagatgagt
    12241 ttttttgatc aatacaatta ttttggctgt tagtaatgga actacttgtg atggtatggt
    12301 gttgcaattc aaaattccct tgcatttggg agaaagcagc acattaagct tataggataa
    12361 agcggtatga gtaattcaaa atggtggttt cttttagtct gtttcattcc tgctattact
    12421 tttgcatctg aggggggaga gcattcagat ccagtcacct ctgttcttct gggtgtcact
    12481 actattttat ttttagctat cattggtcgt tatttagcca gacttcttaa tcaacctggc
    12541 gttcttggtg aattactgat agggatgata gttgggaatc tctcttactt tttcggaagt
    12601 gaattagtag taattcttcg cgaaggttct gccatttatg atgttgtcaa agaaatgtta
    12661 gcaggaattt ctttaaatca ggcggtaact catgttatcc ataattctta ttatgctaac
    12721 caggtgatcc atgccttaag tggagcagag ggaactgaat atcttaaaat agcctatgtc
    12781 gttgatattt tctcacgtta tggtgtcata tttcttcttt ttatggttgg tcttgagacg
    12841 tcgctttctg atttgaaaaa aactggcaaa gaatccattc ttgttgctgt gatcggtatc
    12901 gttgcgccaa tgattttagg gtttgtagtg gcttgtttta tcatacctaa ctcaaattac
    12961 aagattaatt tatttatcgg cgctaccttg agtgctacca gcattggtat tacagcaagt
    13021 gttcttaaag agatgaataa actgagtaca cgtgaagcaa gaacaattct tggtgcggct
    13081 actttggatg atattttagg cctgattatt ttggcggtag tcagcagtat cgttatcagt
    13141 ggtgctgtaa gtatcatggt tgtaatgaaa attatcattt tagccctact gtttttcatt
    13201 ggtaccttgt ttatggggcc ttttgtactt cggaaagcag tcgatttatt ccgttttctg
    13261 gagccttggg aatccaaatt atttatctca ttccttttta ttatggcatt agcctggttg
    13321 gcttcattta tacagttggc ggctataatt ggtgcttttg ctgcgggcgt cattttgcac
    13381 gacgactatt ttggagagca aaaagcccat cataaagatc atcgcagtat tcatcatttg
    13441 gtatctccct tggaaactat attggctcct atgtttttta ctctcattgg gatacaggtt
    13501 aagttagaaa ctttttatga ttggaatgtc attgttttag cgggaggctt atcagtagcg
    13561 gccattcttg gcaagctgct tagtggctta ggcgctaatc gacgggatga taggctgctc
    13621 attggtatag gaatgctgcc tcgtggagag gtaggtttgg tttttgcttc tattggcaga
    13681 actataggcg taataagtga taatctgttt accgcaattg tattgatgat catgatcaca
    13741 acctttattg cgccaccttt attaaagatg cgatatgcca aaccaaaaga ggataggaat
    13801 gcatgaataa tgttttatcg caagttacat ttgatgtaaa acgtgctttg caagaggacg
    13861 tgggcaatgg tgatgttact gcggctttgt tgcctcagca gctgattgta gaagcagaaa
    13921 ttatatcaag agaacctatg cttgtttgtg ggcagccctg ggtaaatgag gtttttaagc
    13981 aagttgataa tacagttgaa atggagtggc gggtttctga aggagatttt ttaagcacgc
    14041 cagctacttt atgtattatt catggtactg ccagtagtat tttaacggca gagcgtactg
    14101 cgttaaattt tttgcaaacc ctttcagcta cggcaacaca aacttatcac tatgttcaga
    14161 aattaaaagg taccaaggca aagctgttag acacacgcaa aacaattcca ggacttcggt
    14221 tagcgcaaaa atacgctgtc cattgtggtg gcgggttaaa tcatcgtatg ggactttatg
    14281 atgcgtttct aatcaaagaa aatcatatca aagcctgtgg ttcagttgct catgctatca
    14341 gtctggcaag gaaaaccgat aaacacttac tggttgagat tgaagtggaa acccttgagg
    14401 agttgcaaga ggctttggat gctcaccctg acaggatatt attggataat ttcactcaag
    14461 atatgcttga gcaagcagtc aaaatgaatc atcccaagta ctgtgaattg gaagcctctg
    14521 gcggcattaa tatcaataat attgcagaca ttgctcaaac cggagtggat tttatttcag
    14581 ttggatccat caccaaatca attcaagcaa ttgatttgag cttattgatt agggaaacat
    14641 tatgagccca agtattgttt ttaccggggg aggcactgcc ggacatgtaa cgcctaatat
    14701 cgctttgatt aaggaatttc gaaaagaagg ctggaatgta gaatatatcg gctctgtttc
    14761 cggaattgaa aaggagatga ttgagccgat ggacattcct tttcatggag tcagtagcgg
    14821 taaattgcgc aggtatttta gtttgaagaa cttgcttgat cctttcaaaa ttgttctggg
    14881 aattattcaa tcttctttgc tattttataa aatcaaaccc gatgtggttt tttcaaaagg
    14941 tggctttgta gcctttcctg tggttgtagg cgcctggtta aatcgaattc ctgttgtcgc
    15001 tcatgagtct gatatgagtc caggacttgc gaatcgccta tcctttcctt tcgtcaataa
    15061 aatatgcctt acttttgatg ctggcaaaaa atactttaag cgtcaggata aaatagaagt
    15121 gacgggtact ccaattcgtc aacagctatt aactggaaat cgaatgaaag gattggagct
    15181 atgcggattt aattcctcca aaccttgcct gcttgtagtg ggaggaagct taggggctgg
    15241 ttcaattaac agttgtattc gaagcgcatt gaaacaattg acatcagaat ttcaagtcat
    15301 tcatctttgt ggcaagggaa aacttgattc ttcattggtt ggtgtggagg gatattgcca
    15361 atttgaatac gccaatgaag agttggctga tctgttcgct gcttcttctg tggtgatttc
    15421 tcgagcagga gctaattctt tgtatgaaat attagcatta ggaaaaccac atatcttaat
    15481 tccaatctct tcacaagtaa gcagaggaga tcaaattcag aatgcaaggt acttccaggg
    15541 attgggaata agcgttgtga ttcaggacga gttattgaaa gctgatgttc tattacaggc
    15601 agtacaggac gtaatgcgaa aaaaagatga aatagataat aaaatcaaag cattaaaaat
    15661 tgagtctgcc actgataaga ttgtggcaat tatcaaggag caagcacatg ttcaaacccc
    15721 aaggattgta tgattacata tgccaacagt ggcaagaaga gatattgcca agtttatgtg
    15781 actatataaa aatccctaat aaatctcctc actttgatgc aaaatgggaa gaacatggtt
    15841 atatggagca ggcagttaat cacattgcca attggtgtaa gtcgcatgct cccaaaggaa
    15901 tgactctgga aattgttcgc ctgaaaaata ggactccatt actatttatg gaaattccag
    15961 gccaaattga tgacactgtg ttgctttatg ggcacttgga taagcaacct gagatgtcag
    16021 gctggagtga cgatttacat ccatggaaac ccgtattgaa aaatggattg ttatacggaa
    16081 gaggaggagc agatgatgga tattctgctt atgcatcact cacggctatt cgcgccttgg
    16141 aacagcaagg tttgccatat cctcgttgta tattaatcat cgaagcgtgt gaggaaagtg
    16201 gcagttacga tttgcctttt tatattgagt tgctgaaaga gcgtattggt aaaccatcat
    16261 tggttatttg tcttgattcc ggagcaggta attatgagca gttatggatg actacgtcat
    16321 tacgcggtaa tttggtcggt aagttaactg ttgaattaat taatgagggc gttcattcgg
    16381 ggagcgccag tggtatagtg gcagacagtt tcagagtagc tcggcaattg atcagcagga
    16441 tagaggacga aaacaccgga gagataaaat tacctcagtt gtattgtgat attcctgatg
    16501 agagaataaa acaagcgaaa caatgtgcgg aaattctagg tgaacaagtt tatagcgaat
    16561 ttccatggat agattctgcc aaacccgtta ttcaagacaa acagcaatta atattaaaca
    16621 gaacatggcg ccctgccttg acggtaactg gtgcagatgg gtttccagcg atagctgatg
    16681 cagggaacgt aatgcgccct gttacgtctt tgaaattatc catgcgtctt ccaccactgg
    16741 ttgatccaga agcagcttct gttgctatgg aaaaagccct gacccaaaac cctccctaca
    16801 atgcaaaggt tgattttaaa atacaaaatg gaggttccaa gggatggaat gctcctttgc
    16861 tttccgattg gttagcgaaa gcggcatctg aagcatcaat gacttattat gataaacctg
    16921 ctgcctacat gggagaaggg ggaaccattc catttatgag tatgctaggc gagcaatttc
    16981 ccaaagcaca atttatgata actggtgttt taggccccca ttccaatgct catggtccga
    17041 acgagttctt acatttggac atggtaaaaa aactcacctc atgtgtctcg tacgttcttt
    17101 atagtttttc acagaaaaaa taatttgaaa ttggctagac tcttgtttga aaaattgaga
    17161 tactcgctcg cggctctgga acgagccgtt tagtccaaca attaggagaa atttatgaaa
    17221 gcaagactca ttgctatttt gttgtcattt attgttatcc ctttctcttc tcaagctgag
    17281 aacgagcctc aaaaaactcc agctaaacaa gtccttgtta agggcaagat taatctcaat
    17341 acagcggatg tgcattcttt aacaggctct tttaaaggga taggaaaaaa acgggctgaa
    17401 gctattattg cttatagaga gaatcatcaa ggctttaaat ctctggaaga gcttgcggaa
    17461 gtcaagggta tagggcagcg ttttgtagat aaaaatagag aaaaactgaa agaaattttc
    17521 gttattaatt aattcacgtg caatataaaa cgtaaatcat gttttataaa gcaaatgaga
    17581 ttgatcccat atgaaagcgt tgcttttact atgggatccg gttaaaagca gttgtttggt
    17641 gtataaaatt cttatgcggc aactgtcgat ttttatccaa atataggata aagtagcaat
    17701 caaaaaaaca acggggtagt actgcgtatg ttcaggaaat taagaggcgt gttttctagc
    17761 gatttatcaa tcgatttggg aacagctaat acattgattt acgtaagaga taaggggatt
    17821 gttctcaatg aaccttctgt agtggcattg cgtaatgaat caggccagaa gcgtgtcgcc
    17881 gctgtaggtc ttgaagccaa acgcatgttg ggaagaaccc ctggcaatat taatgcgata
    17941 agacccatga aagatggggt tattgctgac ttttttgtca ccgagaaaat gttgcagcac
    18001 tttatacaca aagtgcatga aaataaattt ttacgaccca gtccacgcgt tttggtctgt
    18061 gttccttgtg gttctacaca agtggaacgc agagcaattc gtgaatctgc aatgggcgct
    18121 ggtgctcgag aagttttcct tattgaagag cctatggctg cggcattagg ttccggaatg
    18181 cctgttgagg aagccagtgg ttctatggtg gttgatatag gcggtggtac cacagaagtc
    18241 gcaattattt cactaagtgg aatcgtttat catcaatcag tacgtattgg tggcgataaa
    18301 tttgatgacg caatcgtgtc ctacgtacgc cgtaattatg gtactctcat cggagaaaca
    18361 actgctgaac gtattaaaca tgaaattggt tcggctttcc caagccggga tttatttgag
    18421 atcgaggtac gaggtagaaa tcttgcagaa ggagtaccac gtagctttac tttaacaagc
    18481 gctgaaatcc tggaagcact acaggaacct ctatcaggga tagttggtgc ggttcgagct
    18541 gctttggagc tagctcctcc tgagttggct gcagatattg ctgaaagagg tatggtttta
    18601 acaggcggag gcgctctgtt gaaaaatata gacaccttgt tgatggaaga aactggatta
    18661 cccgtattaa tagctgaaga tccattaacc tgcgttgcgc gtggtggtgg taaagcttta
    18721 gaaaccatgg atttgcgcgg cggtgatttc ctctcaacag aataataaac acaatagatt
    18781 atttgctaag ggcggacaca gttctctggg atttgtgttt gcccttgctt tttctattct
    18841 cctgatgttt tctgattatc attatcgata cctggatttc gttcgtagcg gtttttcact
    18901 aatagtttct cccttgcaat atgcggtaga ttatcctgtt cgagtcatcg gctggattca
    18961 gtcgttggtt agtgccaaaa aggcactgat agatgaaaat atgcgtttaa aatacaagca
    19021 aacaatgctt gaagctgaat tgcaaaaatt aattgttatc caaagagaga attcgcaatt
    19081 aaaagaatta ttattgactt catccaaggc tgatatgaga gtcatggctg cccagatact
    19141 cgctgttgat acaagtcagg caagacaagt tgtcgtctta aacaaaggca cacgtgatgg
    19201 cgtctatgtg ggacagccag tgttggatgc caagggagtg atggggcagg ttattgatgt
    19261 aggaccaatg acaagcactt tattactgat ttcagattca aaaagtgctg tgccagtaag
    19321 aaacaatcga accggagaac gcgcaatcct tgtaggcact aatgatattg aagaattgtc
    19381 tttgattaat ttaccgaaga cttcttctat tcatccaggt gatgtgctgg taacgtccgg
    19441 tttaggccga agatatccgg agggctatcc tgtaggacgc gttgaggaag ttaagagtat
    19501 tcctggcgaa gattttgtca aagtcacagt tagccctgtc gcattgttga atcgcaacag
    19561 gttggtgtta ttgatttggg ctgatagtga gcaagaggaa ttaactgctc aaattaatga
    19621 gcgattgaat gctgaggagt ctacagcatg agcgtgcttc acattcgttt aggattgggg
    19681 tttctcgtgg cacttgcttt atcaatactg ccgatgcctg agttaatttc aatttttcga
    19741 cctccttggg tgttattatt ggttttatat attgaatatt atttaccagg taaatttaaa
    19801 ctgactgctc tgttgatagc tggcttatta ctggatgtac tcttatcaac cgtgatcggt
    19861 gaacattcat tcgcattact attggtaacc tggatagcgt caaccaaatc gagacgagtt
    19921 caatttttct cactaattca gcaggtttgt ttgattggat ttttttgttt tctatatcaa
    19981 tcaattatta tatttattga tgctctgtta ggttttaatt ataacctgat aatgcctgta
    20041 gcaagtgcca ttttgggtat gattatttgg ccctggatcc gagtcttggg ttactcttcc
    20101 ttgttaactc gttcagttta tcgttagtaa ggttgattaa cttatattcc ccgggctgga
    20161 gttgatctaa ccgccaatca ccaatctggt gtctaattaa acgtaaagta ggaaatccaa
    20221 tagccgcggt catttttctt atttgatgat ttttcccttc tcttaaaata atttccagcc
    20281 aggtagttgg tatgtgtttc cgttctctta taggcggaat tcgtggccat atttttggct
    20341 ctgaaatcaa cttggcttcg gcgggtaaaa aagtaatgtc tttgattacc aggccttttc
    20401 ttaaaggttg taaatctttg tctgtagggg ccccctctac ttgaacccag taatgtttct
    20461 ttttttcaaa tttaggatgg gttagccggt gttgtagttt tccatcgtca gttaagacaa
    20521 gcaagccttc gctttgttta tccagacgtc cagctgcata aaaacccgga aatttaataa
    20581 atgccgagag agtttgttcg ggtaattcgc cagtaaattg agtcaggact ccataaggtt
    20641 tattaaaaag tattaattgt ggcattttat gattgcgttg attatggaaa ttaatgataa
    20701 attttttggt tattgacagc aaccacttca gggctagatc acgcctttaa gcacaataac
    20761 agcgtaaata attgttcata ttttgtcatt cactcaatga gatatgaggt gcagtaatta
    20821 atcaaaatat gattttgtcc ctcttggaat taaagccaag attgattttt tatggccaat
    20881 cactatacaa ttacacaatt ttattcgtta tggtgactct tattcatgaa atgggtattg
    20941 gttgttatgg caattatcgc tcaaacaagt tgtgcagtga atcacagagt tatggttggg
    21001 gatgaacctg taataattca acaaaccaaa ggtcaaggta agacttttgt acatgttcat
    21061 cacaatgaac aaaccgcact aaaagctgct aaagctgtca ttagaaaaga gggtggaagt
    21121 ctcattacac ttatccattc cggcggtcga aatatagtat tccatttaca taaccaacgc
    21181 tatgaatttg atcccaaccg catttttacc gacaaaggga taaaaaaaac tttgacgcaa
    21241 tacagttatt atactcctga agcgcatcaa gaagtgaaga aacttgcaga taaaattaag
    21301 gccttactac cagagggtaa agtaatcgct gttcataata actcctccta ttctttaaaa
    21361 gattatttgc ctggacatga acttgccaag gatgcaaaag ctttgtatat gaatcctgac
    21421 aattatttta ggaattttta tctggttact aaattaagcg attatctgcg tttaaaaatg
    21481 caaggtttta atggtgtttt acaaaaacct tcggctacag acgatggatc tttatcagtc
    21541 tatctcgcta aacgtgatta tattaatgtc gaggccggtt acgatcagtt ggcagaacaa
    21601 attaagatgt tgcttcatgc ttagtttca
//
LOCUS       DASLOE010000037        21516 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.037, whole genome shotgun sequence.
ACCESSION   DASLOE010000037 DASLOE010000000
VERSION     DASLOE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 21516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21516
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.037"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..189)
                     /locus_tag="U6309_001126"
     CDS             complement(<1..189)
                     /locus_tag="U6309_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504579.1"
                     /translation="MNIKNIPIKPQKIPYYLFLLLLTVGASLILGFLSFGGMYALWPV
                     LPLAFAGFALSVAYEGEIY"
     gene            371..1576
                     /locus_tag="U6309_001127"
     CDS             371..1576
                     /locus_tag="U6309_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEN5504580.1"
                     /translation="MRFNQWMTCITSGLILASNSSFATTSPVHEQLQVPQCLAAKITV
                     PHKILAENKEFKIIDVLSSDVETLTILADKVSCGHFVNVSHKLTGTLAANQQQSAQKL
                     LQKKLVKPFGVSKLHKDVYEIKHEEEVNAALKEIVSDNIWQTLTHMTSYYNRSATKDT
                     GVDTANWLKSKFEQMAVEYGRTDTSTFFVKTGWYKQPSLVTVIGKDIKAPAIVIGAHM
                     DTLDGRMPGAGDDGSGSSSIMEAARVILSSKTTFKRPIYFIWYAAEERGLVGSQHVVQ
                     HFQEQSIPVKAVVQFDMTGYRNDANDPTMWVFTDYTDRDLSNYLAKLIDHYIHVPVDY
                     SRCGYGCSDHASWNEEDIPAAFPCETSFADHNPYIHTSSDKMDLLNLEHMTNFSKLAV
                     AFAIELASE"
     gene            complement(1657..2517)
                     /gene="vipE"
                     /locus_tag="U6309_001128"
     CDS             complement(1657..2517)
                     /gene="vipE"
                     /locus_tag="U6309_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VipE"
                     /protein_id="HEN5504581.1"
                     /translation="MPLTQTQRLINTYGASLKNGTISNEELIILLDPNTFTKSEGYVD
                     PNAPVSDSNHSKMDAIKDFVLTIGPTLDSEILHQLTSRMIELSPPGDRNTFMRGSSLE
                     KAFLAFEMAHYPTKAEEHFNSTRVRTEFPGENDIDNLKAVILNPIIAFFQSPPGTDEK
                     LEKLIKLCTDYKEHLKPSLTDDANNLVNKKHAIVTNMLAALTDSTAPNNATKIKNMEQ
                     ILTEENKATLKDRRDSFSGARFLESILHILSLGIYSKLTKGTFKFWKSHGEALSDNIE
                     EVSKQQPGMK"
     gene            complement(2809..4332)
                     /locus_tag="U6309_001129"
     CDS             complement(2809..4332)
                     /locus_tag="U6309_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEN5504582.1"
                     /translation="MRKKPLSTGAEWTFELIQAYDREIARIAKSFNLDTYPNQIEIIT
                     AEQMMDAYSSVGMPIGYHHWSFGKHFVSVEKSYKRGQMGLAYELVINSNPCISYLMEE
                     NTMAMQALVIAHACYGHNSFFKNNYLFKMWTSADAIIDYLVFAKKYISDCEERYGIDA
                     VEPVLDACHALMNYGVDRYKHPTPLSIQEEKIRQQNREIYMQSQVNELWRTIPFSKQI
                     DKNGQKKRFPEEPQENILYFIEKNAPLLEPWQREIVRIVRKLAQYFYPQGQTKVMNEG
                     WACFWHYTILHALYDEGLVTDEFMLEILQSHTNVIMQPAYNSPYYNGINPYTLGYHMM
                     QDIKRICENPTDEDKKWFPYLANTDWLTSLDTAMRNYKDESFIAQYLSPKLIRDLKLF
                     HIVDDDRNSELYINAIHDEWGYQQIREALSRQYNLGYLEPDIQVYSVDLQGDRSLTLR
                     YSQQNRVPLGSSTNEVLKHLHYLWQFPVILQAVDGENKITEEFSCPPLNKNKTAETN"
     gene            complement(4329..5594)
                     /locus_tag="U6309_001130"
     CDS             complement(4329..5594)
                     /locus_tag="U6309_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HEN5504583.1"
                     /translation="MSQLIDRRQNAGKKSTVNRQRFLRRYKSQIKKAVSEAVGKRSIT
                     EIDQGEQITIPAKDIYEPQFHRGHGGHIERVLPGNDNFIAGDRIKRPGGGGAGGAGGN
                     ASDSGEGEDNFVFELSREEFLELYFEDLELPDLVKKELARISTYKTVRAGVTTSGIPN
                     NINVLRSMKQATGRRVALASPYKKRLKEAEEELERLKQLANPDKIDLLKLERDIEFFK
                     KKIQTVPFIDTIDLRYNHRVRVPSPSTQAVMFCVMDVSGSMDEAKKDIAKRFFILLYM
                     FLTKNYEKIELVFIRHHTSAKEVNEEEFFYSRETGGTVVSSALELLNTIIEARYPPQA
                     WNIYVAQASDGDNWNADSPYCQELLQEKIMPLLQYFAYIEIMPRHHQSLWEVYQQVKE
                     RYPNFAMENIDNVADIYPVFRELFKRKTV"
     gene            complement(5748..7679)
                     /locus_tag="U6309_001131"
     CDS             complement(5748..7679)
                     /locus_tag="U6309_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEN5504584.1"
                     /translation="MNTQDFLAGYTKRFVDNKEEDMSLDEYLELCKTDPTAYANPAER
                     LLMAIGEPELIDTRHDPVLSRIFSNKIIHHYEVFKDFYGMEEPIEQIVGFLKHAAQGL
                     EETKQVLYLLGPVGGGKSSIAEKLKDLMERIPFYAIKGSPVFESPLSLFNPLEDGELL
                     RERFGIPTRYLRYLMSPWAVKRLQEYNGDISKFRVIKVKPSRLKQIAIAKTEPGDENN
                     QDISSLVGKVDIRKLEEFSQDDPDAYSYSGGLCRANRGLLEFVEMFKAPIKVLHPLLT
                     ATQEGNYNATEGLSAIPFEGIILAHSNESEWQSFRNNKNNEAFIDRINIVKVPYCLRV
                     SEEIKIYQKLIHNSSLANAPCAPGTLDMLAQFSVLTRLKEPQNSSIYSKMRVYNGESL
                     KDTDPKAKSYQEYRDFAGVDEGMSGISTRFAFKILSKVFNFDHTEVAANPVHLMYVLE
                     RQIEQEQFPQELHETYLNFIKEYLATKYVDFIGKEIQTAYLESYSEYGQNIFDRYITY
                     ADFWIQDQDYRDPDTGEIFDRALLNLELEKIEKPAGISNPKDFRNEVVNFVLRARANN
                     RGKNPVWNSYEKLKSVIEKKMFTNTEDLLPVISFNAKASEEDKKKHEEFISRMVEKGY
                     TRKQVRLLCEWYLRVRKSQ"
     gene            7852..10443
                     /gene="pepN"
                     /locus_tag="U6309_001132"
     CDS             7852..10443
                     /gene="pepN"
                     /locus_tag="U6309_001132"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEN5504585.1"
                     /translation="MPDTTIYLKNYQPPSFTVETVELNFDLYDDHALVNSTLKLTRQN
                     DGALHLYGEELELIALHLNGRPLEHEKYVLTKDALIIEQCPDEIVLNIVTRIKPQENT
                     QLSGLYRSNHLFCTQCEAEGFRRITYYLDRPDVLATFTTRITADKKQYPILLSNGNLM
                     DTGETADGRHWVIWEDPFKKPSYLFALVAGNLACVRDSFVTCSGRIVDLRIYVEPGNE
                     DKCSHAMESLKKAMKWDEEVYGREYDLDIYMIVAVSDFNMGAMENKGLNIFNSKYILA
                     RPETATDQDFADVEGVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSRDMNS
                     RDVNRIMDVKVLRSAQFPEDAGSMAHPVRPESYQEINNFYTATVYNKGAEVIRMQHTL
                     LGKERFRRGMDLYFKRHDGYAVTIDDFVAAMEDANGVDLTQFKLWYSQAGTPEVRVLS
                     HFSEGRLEITMQQNCPPTPECHEKKPFHIPVRIALFDLKGEMIPIENELLELKEKEQR
                     FIFSGLNEKPVVSLLREFSAPVKIYDDLDQDDLLFLLRYETDGYAKWDAAQRLVLNCL
                     NENLKLPASEWQVSKTLVAAFQHVLLDDSLDMDLRAELLTPPGFEEVAATMKWVDVSK
                     VESVRDYFRQQLGLGLYTEASDLYKELWEVEDHRMHGQAYGRRKLRNVCLWLMMKANE
                     SDALELCREQFKASRTMTDQIASCSLLVNCSRESQRNEAIEGFYKQWSHNELVLDKWF
                     AMQAACELPGTLDHVKKLTQHPAFCIKNPNKVRALVGAFCMANPRNFHALDGSGYVFL
                     SEVLIKLDKLNPQIAARLATPFTRWRSYDEPRQKLIQNQLEQLTKLDLSRDLREVVDK
                     SLIVEEK"
     gene            10653..11450
                     /gene="aroE"
                     /locus_tag="U6309_001133"
     CDS             10653..11450
                     /gene="aroE"
                     /locus_tag="U6309_001133"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEN5504586.1"
                     /translation="MLRRFAVIGHPIAHSLSPVIHQMFAQQTQIELIYEKILGDDVKF
                     EQQISDFFIQYGNGLNVTLPYKKRAYELAKIRTQRCTLAGAANTLWMEENQLHADNTD
                     GIGLIRDLSRFLELKDKKILILGAGGAARGIIFPLLEAKPLQLIVANRTLEKAEELQR
                     QFPQINVTSFAELTEFFDLIINATSTSLSGQVIVLPEEVLSHKPFCYDLAYNQKTSTA
                     FVQYARNGGCEAVDGLGMLVEQAAEAFFTWNKVMPSTQKILEHLRSF"
     gene            complement(11500..12612)
                     /locus_tag="U6309_001134"
     CDS             complement(11500..12612)
                     /locus_tag="U6309_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN5504587.1"
                     /translation="MMVKKALYLAGGGARGAYQAGVLKAIGHILQTKTLPFDLVSGVS
                     VGSVNAAVLAENANDFPAALEKLEAIWGEIHCQQIYKASNYELSKSVMRNLSTLIIKQ
                     RQSGHLLDTTPLRQFLEESIDFTRIEQNIQEGYLDTFEVLSACYETHQTISFYAHNHS
                     EFEDWNYPRHSSQRAVINAQHILASSALPLFFPPVSLNGFHYGDGSIGLVAPLRGAIR
                     FQVDRILILGTRELPEFTDPETLRNGEIAFAHILGNMMNGLFLDNLDRDIEMVNRMNE
                     IATLLSMWKKRRSPWRPISTLHLRPSRAMASVAQSHYTSMPVLLRFLLNIMGARSHSG
                     DLLSFLLFEKEFTRELLELGYQDTITAATEVSAFFS"
     gene            12750..14132
                     /locus_tag="U6309_001135"
     CDS             12750..14132
                     /locus_tag="U6309_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504588.1"
                     /translation="MGITKDERFRKFEIEKTLQLLQSMQRPIEYPLTPIDKTSLLKKA
                     TKRIPLLSKFLKTVDDTGEVVVKIGDRMTSLSHTPGKESLAHGFAFGGVAMAGFDFLR
                     IPVIYLSAYLLGEEVPVNLSNNAKWFYSALLLGLTITALAVPVTAPYIAFASAGLSLG
                     VGTFLLGKTLYERYQLGRERRKLKAEILREEDEMLKIQDKAKHLESLLQSADNEEQIA
                     AILYEISVVQEDYSRQKERLQSLKNDELQLEQQIKKLGTMKVVDRTIAVALSALTIAA
                     LAVTLFFPPVGGGMLAGIAIFGGAYFLARVSMPLIKATAVWLYNKLKSTPSESSESQD
                     IDNKPTLSNEKEEKKEDSLQIDNEFVLPPIDAEASISEDSKEYQPVHESTEDVLVRLS
                     VNIDRLKEKVRMEKKEEIEEESPEELQHFSSLTPSPRTLGSRKLSAKESDDENGESES
                     DIEHPDVPKI"
     gene            14200..15702
                     /locus_tag="U6309_001136"
     CDS             14200..15702
                     /locus_tag="U6309_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5504589.1"
                     /translation="MLNLFREQPRAFHMIFMLELWERFGFYTVQGILTLYFIRYLGYS
                     DLESYYTFGAYSALVYGMVVIGGYLGDRILGTKRTIVLGLVVLAMGYFSLAVTDKQHV
                     FLALGLICVGNGLFKANPSNLLSKCYDDNDPRLHGGFTLYYMAINLGSMVALFVGPAV
                     SSAYGYSYAYMISAIGLILGIVNYWYQRQHVSHVNTKADLRKITVFHWGIIIAGVLIL
                     TGISAYLLQHVMLAKNLLWGITLLVVSVYIYLMRKQAQASFMRMLVALVLMIEAVIFF
                     TLYQQMPTSLNLFAVNNVIPTFLGITIDPQSFQALNPIWIVLMSPVLAYFYRKLHQHN
                     ILFSIPYKFAFGMTMCAVSFLMLYFARYFYVGNGMVSSWWMIFSYLFQSLGELLVSAL
                     GVAMVAELVPATNAGFVMGMWFLTSAVAGFIGATVASFTALPENIKPGVESLLIYSDV
                     FACIGLVTLLIAVIMWLVSPRLSRYITVPSLEGDKDIETVEYSTTYIPSH"
     gene            15808..17211
                     /gene="lem29"
                     /locus_tag="U6309_001137"
     CDS             15808..17211
                     /gene="lem29"
                     /locus_tag="U6309_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem29"
                     /protein_id="HEN5504590.1"
                     /translation="MYKICTCNQSFPNHYRVKVFRFCELICLEYKVGLEKKIKLSQRS
                     LELLNDYLNLGGSGPSNLVNGSSSIDFSSLTQLLASNLSKSSKISKTIKKKREFIEAA
                     LYAELLRDLSTSLNGSDEEIKPAEKEPPVSNKLKFWLLAIAGTLLAACEGFDSITTMM
                     AVLSFPAWVVLCAGILFSALSVVVFYGFDLVQVSQNLEIKLTDTPKLLDLYLSQMEEI
                     KAIRKKIDNYSLASMSMDELNELHLILVMLQDRVNSLVKAGKKFDLALNSPKMDVAKT
                     IVSGVSGILFFGGGFFAGQSVATFMLALVMTSVTPTFWPVLLFSVIVGLAAFSLYWYV
                     QRVGVNKLVSEWFGLDEDKIEKLCGKSKMDKELKKLNNLDEKVMTTSDLLSKVANLES
                     KLIDIGKQMKEMSAHQTPEKKLEGAAGLKASSNVYSFHFNSSNRDQESVYGERVLDDA
                     VSDYGRGQNNDSIHSLC"
     gene            complement(17099..17896)
                     /locus_tag="U6309_001138"
     CDS             complement(17099..17896)
                     /locus_tag="U6309_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504591.1"
                     /translation="MNRQDVKAIHSYVVDEEKKKISLKLINKLYRFLSKYYHLASQEE
                     FIRFIIQPDLYGELTVLYGENKEIAGFSRNCRQSVDLGKKQVTCYIAYLYLNPEYQIH
                     ASIKSAGLTQAIKYKLANPHEELVYIAFANNPLTYEFIYQLSDLIYPKPLQRVPDQIL
                     TVLNALKKQNGWISTNNHPMVVNSPLVPLRSQLSRINEESSELKEFYMSANPDYLQGN
                     SLLIYMPLHLANIGYGLNHYSDPYHNQKQHHQGPSHRKRSPDPDLRN"
     gene            complement(18179..18871)
                     /gene="hda"
                     /locus_tag="U6309_001139"
     CDS             complement(18179..18871)
                     /gene="hda"
                     /locus_tag="U6309_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEN5504592.1"
                     /translation="MNKQLALAIKLNDEATLDDFNWGNNTLLQQQLIEMLSFKADRLL
                     YLWGPKGSGKSHLLQACCQAINLTQSAIYLPLAFLKEWGPQSIEGLEDQTLICIDDIN
                     TIANDSAWEEALFHLYNKIKDSERSLLIISGNQPPIKCDIKLADLRSRLSWGLVIQLN
                     ELNDDEKINTLKMRAAKRGFELPESVGHFLLNRCSRNMHDLYEILNRLDDASWEAHRK
                     ITIPFVKNILKI"
     gene            complement(18868..19428)
                     /locus_tag="U6309_001140"
     CDS             complement(18868..19428)
                     /locus_tag="U6309_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN5504593.1"
                     /translation="MILKHIPNTLTLLRLVLIAPFLMYLFHHEYVKAFYIFIAAGLTD
                     GLDGWLARHFQWQSFFGSFVDPLADKLLVASSFISLALLSSLPWWLVLLVFFRDFTIS
                     MGVLAWYYFIQRKLDFEPTRLSKLNTGFQLTLVTLCLFELAYFRFPYDLVDILIYLTA
                     FTTVTSYLDYVWTWSRKAWSPKESLK"
     gene            complement(19688..21415)
                     /gene="frgA"
                     /locus_tag="U6309_001141"
     CDS             complement(19688..21415)
                     /gene="frgA"
                     /locus_tag="U6309_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore biosynthetic protein FrgA"
                     /protein_id="HEN5504594.1"
                     /translation="MALAYGNFHELSHQLRFLLFEIGIGLPQNSVDYFITLAHKNTLN
                     RLQHASIKEGLIQSAIASHHVHDFIDQLQIKLKNSMPESKFFQWRKIREALDESIANE
                     ALAYAYRQNWNTQLRNEAMHYKSLWTWINNELSPYQTLLFLEQWGSLRHPYHPAFSAK
                     TGFTRREVLQNSPEFQAKVSVHWCALNKTKIQSINPKIDYANQISQEFPKEYFYWREK
                     LLFSHINPDDYYPIPVHPWHWRNQLQMAFASLIDNKSLILLPHHQTLMPSLSPDIMMP
                     TQSTQCTLKLATTLSTSMAGKLNNSDDMVLLTRWIDSLLAKTNYYQNTLFICKNLESM
                     SAYDQTLSECNRVKLLFGLYQNPLHKIRPDQRAVPLPALLTDSPCSNTPLLIEIIKAS
                     ELHPTTYFTEYCYKILFGQLHLLLKYGLALEVEQHNILVIFDDNKPQGIIIKEPNNLK
                     LCNHELFKNVQKPNAPDSLSIYTKDLNQVRTLFIQGTLKNHLHHLIGCLCNEYQIPSR
                     TLWGLTRQVMKTVFKDLSKDIDPRILSWQQHLLLHDNWEHQPELLLSLHSKLNQNITI
                     KEYNPLSEI"
BASE COUNT         6273 a         3824 c         4449 g         6970 t
ORIGIN      
        1 atagatttca ccttcataag ctaccgataa cgcaaaacca gcaaaagcca aaggtaacac
       61 aggccaaaga gcgtacatgc ctccaaagct taaaaagcca agtatcaggc ttgctccgac
      121 agttaatagc aaaagaaaca agtaataagg gattttttgg ggcttaatag gtatattttt
      181 tatattcatt gtagtatatt tgctcattga tgcgtaatgc agaactttat accaaatgaa
      241 aatgattatc aatagcatta atattaaaaa agtatttttt gtctttgttt caattcaaca
      301 ataaagtttg tttgcaatat tcgtttcatc aggtagtatc aaaaacgatt taaataactt
      361 aggagaggat atgcgtttta accaatggat gacctgtatc acatcaggat taatactcgc
      421 cagtaattca agttttgcaa cgacgtcgcc tgttcatgag caattgcaag tcccacaatg
      481 ccttgctgca aaaataaccg taccccataa gattttggcc gagaataaag aattcaaaat
      541 tattgatgta ctttcatctg atgtagagac cttgactatt ctggctgata aagtcagttg
      601 cggccatttt gtgaatgtga gtcataaatt aacgggaacc ttagcggcaa accaacagca
      661 atctgctcaa aagttactgc aaaaaaagct cgttaagccc tttggtgtat ccaaattaca
      721 taaagacgtt tatgaaatca agcatgaaga agaagtcaat gcggcactaa aagaaattgt
      781 ctctgataac atatggcaaa ctctgactca tatgacttcc tattataacc gctctgcaac
      841 aaaagatacg ggtgttgata cggccaattg gttaaaatcc aaatttgaac agatggccgt
      901 tgagtatgga cgaactgata cctcgacctt ttttgtaaaa acagggtggt ataaacaacc
      961 ttccctggtt accgtgattg gcaaagacat caaagccccc gctattgtta ttggtgctca
     1021 tatggatact cttgatggcc gtatgcctgg ggctggtgat gatggaagtg gatcatcgag
     1081 cattatggaa gccgcacggg ttattttgtc ttctaaaaca acatttaagc gccccattta
     1141 ttttatttgg tatgctgctg aagaaagagg tttggtcggt tcacaacacg tagtccaaca
     1201 ctttcaggaa caatcaattc cagtgaaggc tgtggtacaa tttgacatga ccggttatcg
     1261 aaatgatgcc aatgatccaa ctatgtgggt atttactgat tacacagata gagatttaag
     1321 taattatttg gccaaattaa ttgatcatta tattcatgtg ccagtagatt attcacgatg
     1381 tggttatggt tgcagtgatc atgcttcctg gaatgaagaa gatattcctg ctgcattccc
     1441 atgtgaaacc agtttcgctg atcacaaccc atacatccat acttcttctg ataaaatgga
     1501 tttattgaac ctggaacata tgaccaattt ctccaaactc gcggtagctt tcgctattga
     1561 actcgcttcc gagtaactgt tttaatgcct ctcataaatt ttaaatattt ttgagaggct
     1621 attttttaaa gttaatttat tgatatcgat tgatatttat ttcatgccag gctgctgttt
     1681 actaacttct tcaatgttat cggagagagc ctcgccatgg cttttccaaa atttaaaagt
     1741 gcctttggtc agttttgaat aaatacccag actcaatatg tgcaggatac tttctagaaa
     1801 tctagcaccg ctaaatgaat ctcggcgatc ttttaaggtt gctttgtttt cttcagtcag
     1861 tatttgctcc atatttttaa tttttgttgc gttatttggg gctgtgctgt cagtgagggc
     1921 ggctagcatg tttgtaacaa tcgcatgttt tttatttact aaattattag cgtcgtcggt
     1981 tagagaaggt ttcaagtgct ctttataatc cgtgcataat ttgataagtt tttctaattt
     2041 ttcatcggtt ccgggaggcg attggaaaaa tgcaatgatt gggttcaaaa taactgcttt
     2101 aagattgtct atatcatttt cgcctgggaa ttctgttctc actcttgtcg agttgaaatg
     2161 ctcctctgct tttgttgggt agtgagccat ttcaaaagct aagaaagctt tttccaagct
     2221 agagcctctc ataaatgtgt ttctatctcc ggggggcgat aattcgatca ttcgagatgt
     2281 taattggtga agtatttcag aatctaaagt cgggccgatg gttaatacaa aatcctttat
     2341 tgcatccatt ttactatgat tggagtctga aaccggggca ttgggatcaa cataaccttc
     2401 tgattttgta aaagtgtttg gatccagcaa aataatcagt tcttcgttag agattgttcc
     2461 atttttaagt gaagcgccgt aggtattaat caggcgttgg gtttgagtta aaggcattgc
     2521 agtgactcca atgattggtt atggcaaatt atagtacaaa attattaaga aattattagg
     2581 agagtatttc tattgccaca acccgcatta cctcaatgaa aggtattgtc taaggagcag
     2641 ccagataaaa ttgtttcgtt gcttcaagat cgcatcagga gcagtcatta gatcgggtgg
     2701 ttggcagcat attaagggca gtgaattaag gagaagaaaa agaatagata ctggccctta
     2761 gttataatag aaaactaata gctattttga gccagtaata taagactatc aattagtttc
     2821 cgctgtttta tttttattaa gaggtgggca actaaattcc tcggtgattt tattttcccc
     2881 atcaactgcc tgtaatatga ctggaaattg ccataaataa tgcaagtgct ttaatacttc
     2941 atttgttgag cttccaagtg gtactctgtt ttgttgggag taccttaaag tcaaagaacg
     3001 atctccttgc aaatcaacgg agtagacttg gatatcaggt tccagataac ccagattgta
     3061 ttgtctggat aatgcttctc tgatttgctg atatccccat tcgtcgtgaa tggcattgat
     3121 gtataattcg gagtttcggt cgtcatcaac aatatggaac agttttaaat ctcgtataag
     3181 ttttggtgac aggtattgag cgataaaact ttcatctttg tagttgcgca tcgccgtatc
     3241 aaggcttgtg agccaatcag tattcgcgag atagggaaac cactttttgt cttcgtctgt
     3301 tgggttttca caaattcgtt tgatgtcttg catcatatgg tagccaaggg tgtatggatt
     3361 aataccattg taataagggc tattataggc tggttgcatg atgacattgg tatgactttg
     3421 cagtatttcc agcataaatt catcagtcac taaaccttcg tcatacaaag catgcaaaat
     3481 agtataatgc caaaagcagg cccacccttc attcatcact ttagtttgtc cctgaggata
     3541 aaaatattgg gccagttttc ttactatacg cactatttct ctctgccaag gctctaataa
     3601 aggggcattt ttttctataa aatagagaat attttcctga ggctcttctg gaaagcgttt
     3661 tttttgtccg tttttatcga tttgcttact gaaaggtatc gttctccata gctcattaac
     3721 ttgtgattgc atgtagattt cacggttttg ctgtcgaatt ttttcttcct ggatagataa
     3781 gggtgtggga tgtttgtacc gatcaacccc gtaattcata agggcatggc aagcatcaag
     3841 cactggttct accgcatcta ttccgtagcg ttcctcacaa tcgctgatgt atttcttggc
     3901 gaataccaga tagtcaataa tagcatccgc agaagtccac attttaaata agtaattgtt
     3961 cttaaaaaaa gaattgtggc catagcatgc gtgagcgata accaaggctt gcatagccat
     4021 ggtattttct tccattaaat aagaaataca gggattggaa ttaattacaa gctcataggc
     4081 caggcccatt tgtcctcttt tgtagctttt ctcaacactg acaaaatgtt ttccaaaaga
     4141 ccaatggtgg taaccgatag gcatacccac cgaagaatag gcatccatca tttgttcagc
     4201 ggtaatgatt tcaatttggt ttggatatgt atccaaatta aagcttttgg cgatgcgtgc
     4261 aatttcgcga tcataggcct gtattaactc gaaagtccat tctgcaccag ttgatagagg
     4321 cttttttctc ataccgtttt ccttttaaat aattcgcgaa ataccggata aatatcagca
     4381 acattatcaa tattttccat ggcaaaatta ggataacgtt cttttacttg ttgatatact
     4441 tcccacaaac tttgatgatg acggggcata atttcaatat aggcaaaata ttgcaataaa
     4501 ggcataattt tttcttgcag caactcctga caataaggag agtcggcatt ccaattatca
     4561 ccatctgatg cttgggcaac ataaatattc caggcttgag gagggtagcg tgcctcgatg
     4621 atggtgttta ataactccag ggcactggaa acaacagtcc cgccagtctc gcgcgaataa
     4681 aaaaattcct cttcattaac ttctttggct gaggtgtgat ggcggataaa aaccaattca
     4741 attttttcgt aatttttggt aagaaacata tagagtagga tgaaaaagcg ttttgctatg
     4801 tcttttttcg cttcatccat ggaacctgat acgtccatta cgcaaaacat cactgcttgt
     4861 gtagatggtg aaggcacacg cacacggtga ttataacgca gatcgattgt atctataaat
     4921 ggtacagttt gtatcttttt tttgaaaaac tcaatgtctc tttcaagctt caataggtct
     4981 attttgtctg gattggccaa ttgtttcagc cgttcaagct cttcttctgc ttcctttaat
     5041 ctctttttat agggggaggc taaagcgaca cggcgtcctg tcgcttgttt cattgagcga
     5101 aggacattaa tgttgttagg tattccacta gtggttacgc cagcccttac tgttttataa
     5161 gtgctaattc ttgctagttc tttttttacc aaatcgggta attccagatc ttcaaaatag
     5221 agttccaaaa actcttctcg agataattca aaaacaaaat tatcttcgcc ttcaccacta
     5281 tcgctggcgt tacctccagc tcctccagca cctccaccgc cgggtctttt tatcctgtct
     5341 ccggcgatga agttatcatt tccaggtagt acacgttcga tatggccacc atggcctcta
     5401 tggaattggg gctcataaat atctttggct ggtatagtga tttgttcgcc ttggtcaatt
     5461 tcagtaatac tccgttttcc gacagcctca gagactgctt ttttaatttg gcttttataa
     5521 cggcgtaaaa aacgttggcg attaaccgtg cttttttttc cagcattttg tcgtctatca
     5581 attaattgcg acataattgt tagctccaca ccttctgcag gtcagttttt tgttagccag
     5641 gttgtaagca gtatctgaat ttaggattct acttgatctt ttcagatagt tacttattaa
     5701 ccctgactgt atttcataac ctattgagtt atgaatgatc ctacctatta ttgtgacttc
     5761 cttactctca aataccattc acaaagtaag cgaacttgtt ttcgtgtgta gcctttttct
     5821 accattcgag agataaactc ttcgtgtttt ttcttatctt cctcagatgc tttggcattg
     5881 aaagaaataa cgggtaacaa atcttccgta ttggtaaaca tttttttctc aatcactgat
     5941 tttagttttt catagctatt ccatacaggg ttttttcctc ggttatttgc tcgagcgcgc
     6001 agtacgaaat tgactacttc attacggaag tctttaggat tggagatccc tgccggtttt
     6061 tcgattttct ccagttccag gtttaataag gctctgtcaa atatctcgcc agtatccgga
     6121 tcgcgataat cctgatcttg aatccagaaa tcagcatagg taatgtaacg gtcaaatatg
     6181 ttctgcccat actcagaata ggattccagg taagcggttt gaatttcctt gcctataaaa
     6241 tcgacgtatt tggtcgccaa atattcttta atgaagttca agtaggtttc atgtaactct
     6301 tgaggaaatt gttcctgttc gatttggcgt tccagcacat acattaaatg aaccgggttg
     6361 gcggccactt cagtatgatc aaaattgaat acttttgaaa ggattttgaa ggcaaaccgc
     6421 gtggatattc cgctcatccc ttcgtcaacc cctgcaaaat ctctatactc ctgatacgat
     6481 tttgccttgg gatcggtatc tttcaaactc tctccattat acacgcgcat tttggagtaa
     6541 atactggagt tttgtggttc ttttaaacgg gttaatacag aaaactgggc tagcatatcc
     6601 agtgtaccag gtgcgcaagg ggcatttgct aaagaactgt tatggattag tttttgatag
     6661 attttgattt cttcagaaac acgcaggcaa taaggcactt tgactatgtt gattcggtct
     6721 ataaatgcct cgttattttt attgtttcta aatgattgcc attctgattc attggagtgg
     6781 gctagtataa ttccttcaaa aggaatagct gaaagccctt ctgtggcatt gtaattccct
     6841 tcttgcgttg cggtcagcaa gggatgaagg acctttatcg gtgccttaaa catttcaacg
     6901 aactccaaaa gtccgcggtt agctcgacac aagcctccag agtagctgta agcatcagga
     6961 tcatcttggg aaaattcttc caatttacgg atatcgactt taccaaccag tgaagaaatg
     7021 tcttgattgt tttcatcgcc aggttctgtt tttgcaatgg caatttgttt gagtcgagaa
     7081 ggcttgactt taataacccg gaatttgctg atgtcgccat tgtattcctg taatcgcttg
     7141 actgcccagg gggacattaa gtagcgtagg tagcgtgttg ggataccaaa acgttctcta
     7201 agtaattcac catcttccaa ggggttaaat aatgacagtg gagattcaaa gacaggagaa
     7261 cctttgattg cataaaaagg gattctttcc attaaatctt tcaatttttc tgcaatggaa
     7321 gatttaccac cgccaacagg ccccaaaaga tataaaacct gttttgtttc ctctaaccct
     7381 tgcgcagcat gtttcaaaaa gccaacgatt tgctcaatgg gttcttccat tccataaaaa
     7441 tctttaaaaa cttcataatg atggattatt ttatttgaga aaattcgtga tagaaccgga
     7501 tcatgtcggg tatcgatcaa ttcaggctcg cctatcgcca tgagcaaacg ttctgctggg
     7561 ttggcatatg ctgtcgggtc cgttttgcac aactccagat attcatccaa actcatgtct
     7621 tcttctttgt tatcaacaaa tcgtttggta taacccgcta aaaaatcttg tgtattcatg
     7681 cgccatcccc ttttacttaa ccctgagaag gatataatgt gattcttttt tttctctttc
     7741 ctgtataaaa gcaaaaatta cttcttttaa ttcatagtat agtagactaa ccacttaatt
     7801 taatacataa atcaataaac ttattcgttt tttttatagg aagcaatgac tatgccagac
     7861 acgaccattt atttgaagaa ttatcaaccg ccttctttta cagttgagac tgtggaattg
     7921 aattttgatt tgtatgatga ccatgctcta gtgaacagca ctcttaagtt aacacgtcaa
     7981 aacgatggcg ctttgcattt gtatggcgaa gagctggaat taattgcttt gcatttaaat
     8041 gggcgacctt tagaacatga gaagtatgtg ttgacgaaag atgctcttat tatagagcaa
     8101 tgcccggatg aaatagtact taatattgtg actcgcatta aacctcaaga aaatacgcaa
     8161 ttatcaggac tttatcgttc taaccattta ttctgtactc aatgcgaagc agaaggtttc
     8221 agacgaatta cctattatct tgatagaccc gatgttttgg caacttttac tacacgaatt
     8281 actgcagata aaaagcaata tccgatttta ttgtctaacg gtaatttaat ggataccgga
     8341 gaaaccgcgg atggacgtca ttgggtgatt tgggaggatc ctttcaagaa gccctcttat
     8401 ttattcgctt tggtagcggg caatttagct tgcgttagag atagttttgt tacttgctcc
     8461 ggcaggattg ttgatttacg gatttatgta gaaccgggga atgaggacaa gtgctctcat
     8521 gccatggaat cattaaaaaa agccatgaaa tgggatgaag aggtttatgg ccgtgagtac
     8581 gatttagata tttatatgat agttgcggtg agtgatttca atatgggagc aatggaaaac
     8641 aaagggttga atatatttaa ttccaaatac atcctcgctc gccctgaaac cgccactgat
     8701 caagatttcg cggatgttga aggtgtagta ggccatgagt atttccataa ctggacagga
     8761 aatcgtgtga cttgtcgcga ttggttccag ttaagtttaa aagagggatt gactgtattc
     8821 cgggatcagg agttttctcg ggatatgaat tctcgtgatg ttaatcggat tatggacgtt
     8881 aaggtattgc gcagtgctca gtttcccgag gatgccggga gcatggcgca tccagtaagg
     8941 ccggaatcct atcaggaaat taataatttt tacacggcga cagtatataa caaaggcgcg
     9001 gaagtgattc gcatgcaaca tactttgtta ggcaaggaga ggtttcgacg aggcatggat
     9061 ctttatttca aacgccatga cggctatgcc gtaaccattg atgattttgt tgctgccatg
     9121 gaagatgcca atggagttga cttaacgcaa ttcaaactct ggtatagcca ggcagggaca
     9181 cctgaagtgc gcgttctcag ccatttttca gaaggacgat tggaaattac catgcaacaa
     9241 aattgtcctc ctacgcctga gtgccatgag aaaaagccat ttcatatccc ggttcgcatt
     9301 gcattatttg atttgaaagg ggaaatgatt cccatagaaa acgagcttct ggagttaaag
     9361 gaaaaggagc aacgttttat tttttctggg ctaaatgaaa agcctgttgt ctcgctatta
     9421 agagaattct cagcccctgt gaaaatttat gatgatctgg atcaggatga cttgttattt
     9481 ttattgcgtt atgaaacgga tggatatgcc aagtgggatg cggcacaacg cctggttctt
     9541 aactgtctca atgaaaactt gaaattgcca gccagtgaat ggcaagtctc taaaactctt
     9601 gttgctgcct ttcagcatgt tctgcttgat gactctcttg atatggatct cagggcagag
     9661 ttgttgacac ctccaggatt tgaagaagtg gcagctacca tgaaatgggt tgatgtcagc
     9721 aaggttgaat cagtgcgaga ttatttccgt caacaattgg gattgggctt atacactgag
     9781 gcgtctgatc tttataaaga attatgggaa gtagaagatc atcgcatgca tggccaagct
     9841 tatggacgca gaaaattaag aaatgtttgt ttatggttga tgatgaaagc gaacgaatca
     9901 gatgctttag aattatgccg agaacaattc aaggcttctc gtaccatgac ggatcaaatt
     9961 gccagttgca gcttactagt caattgctcc agggaatctc aacgaaatga agccatagaa
    10021 ggattttata aacaatggtc tcataatgaa ttggtactgg ataagtggtt tgcgatgcaa
    10081 gcggcatgtg agttgccagg cacattggat catgtcaaaa aactaactca acatcctgct
    10141 ttttgtatta aaaatccgaa taaagttcgg gcgctggttg gcgctttttg tatggctaac
    10201 cccagaaact tccatgcgtt ggatggcagc ggatatgtct ttttaagtga ggtattgatt
    10261 aagctcgaca aactaaatcc tcaaatcgcc gcacgcttgg ctacgccttt tacgcgttgg
    10321 cgaagttatg atgaaccaag acaaaagtta atacagaacc agttggagca gcttacaaaa
    10381 ctcgatttgt cgcgggattt acgcgaggtt gtagataaga gtctgattgt tgaggaaaaa
    10441 taggatgaat taaagtgtgc tggattttgc tataggcaga ttcagcacga cttaaacgaa
    10501 tttgtgcaaa caaagctttc aaaattcatc aactaacttt tcagcttgat caccgggtgc
    10561 gatttaagaa caacttggat tgatgtgctt gttgcgtgag aacagtttat aattaggaaa
    10621 accaatgaaa cctataggat aaaggagttt ttgtgcttcg tcgttttgca gtgataggtc
    10681 atccaattgc acatagtttg tctcctgtca ttcatcaaat gtttgcccaa caaacccaaa
    10741 ttgaattgat ttatgaaaaa atcttgggtg atgatgtcaa gtttgaacaa caaatctctg
    10801 atttttttat ccagtatggc aacgggctta atgtcacttt accttataaa aagcgcgctt
    10861 atgaattagc aaaaatacgc acgcaaagat gcaccctggc tggagcagca aacaccctat
    10921 ggatggagga aaatcaatta catgctgata ataccgatgg tattgggttg atacgtgatt
    10981 tatcccgatt tcttgagtta aaggataaaa aaattctgat tctgggggct ggcggagctg
    11041 caagagggat tatttttcct ttattggaag cgaaaccgtt acaattaatt gttgctaatc
    11101 ggactttgga aaaagcagaa gaattacagc gtcaatttcc tcaaatcaat gtcacaagtt
    11161 ttgctgaatt aacagaattc tttgatttaa tcatcaatgc cacttctacg agtttgtccg
    11221 gtcaggtcat tgtattacct gaagaggtgt tatcccataa accattttgt tatgatttgg
    11281 cttataatca aaaaacaagc accgcctttg ttcaatatgc tcgtaatgga gggtgcgaag
    11341 cagttgatgg attaggtatg ttagtagagc aagctgcgga agcattcttt acatggaaca
    11401 aagttatgcc ttctacccaa aaaatattag aacacctgcg atctttttaa attggaagct
    11461 tgcataacaa ctagatgcac tttggcaacc aaactctggt taactaaaaa aggcgctcac
    11521 ttctgtcgct gcagtaatgg tgtcctggta tcctaattcc agtaattccc gagtaaattc
    11581 tttttcaaag agtaaaaagc taagtagatc accggaatga cttctggccc ccataatatt
    11641 taataaaaat cgaagcaata ctggcatgct ggtgtaatgg ctttgggcta ctgaggccat
    11701 tgctcggctt gggcgtaaat gcagggttga gattggtctc cagggagagc gtcgtttttt
    11761 ccacatagat aagagtgtcg ctatttcatt catacgattg accatctcaa tgtctctatc
    11821 cagattatcc agaaaaagcc cattcatcat attgcctaga atatgagcga aagcaatttc
    11881 accattacgc aaggtctctg gatcagtaaa ttcgggcaat tccctggtac ctaagatgag
    11941 aattctatcc acttgaaaac gaatagcacc gcgaaggggg gcgaccaagc ctatgcttcc
    12001 atcaccataa tggaatccat tgaggctgac agggggaaag aacaatggta aggcgcttga
    12061 tgccagaata tgctgggcgt tgatcacggc acgttgactg ctatgacggg gataattcca
    12121 atcttcaaat tctgaatgat tatgagcgta gaaagagatg gtttgatgtg tttcataaca
    12181 ggcgcttaaa acttcgaaag tatcgaggta tccttcttgt atattttgtt caattcgtgt
    12241 gaaatcaatg ctttcttcca gaaattgccg taatggtgtt gtatcaagca aatgccctga
    12301 ttgacgttgt ttgataatca aggtgcttaa attacgcatt actgatttgc ttaattcgta
    12361 gttactggct ttgtagattt gttgacaatg aatttcaccc caaatagcct cgagtttctc
    12421 cagggcagca ggaaaatcat tggcattttc agcaagtacg gccgcattga cgctgcctac
    12481 gctgacgcca ctcactaagt caaaaggtag ggttttggtt tgtaaaatat gaccgatagc
    12541 tttgagtacg cctgcttgat aagcgcctcg agcaccacca cctgccaaat atagagcttt
    12601 tttaaccatc atgttttatt ttatttatta taacttgaaa tatagttgaa atctgggcta
    12661 attagcaaga aagaattatc gatgtcatct tttcttaatt atgaattgat atacttaaat
    12721 taaatcgatt cttttcttga gtcattatca tgggaattac aaaagacgaa cgatttagaa
    12781 aatttgaaat agaaaagact cttcaactct tacagtcgat gcaacgtcct attgaatatc
    12841 cgttgacgcc tatagataaa acgtcactat taaaaaaagc cacaaaacgc attcctttgc
    12901 tgtcaaaatt tttaaaaact gttgatgata cgggtgaagt agttgtcaaa attggtgata
    12961 ggatgaccag tttatcccat acacctggta aagagtcttt ggctcatggt tttgcctttg
    13021 gtggtgttgc catggcaggc tttgattttc ttaggattcc tgtcatttat ttgtcggctt
    13081 atttgctagg cgaagaagtc cctgttaatt tgagtaataa tgccaaatgg ttttattccg
    13141 ccctattgtt aggattgacc ataaccgcat tggcagtccc ggtaacagct ccttatatag
    13201 cttttgcttc agcaggcctc agtttaggtg taggtacgtt tttattagga aaaactcttt
    13261 atgaacgcta tcaattaggt cgggaaagaa gaaaattaaa agcggagatc ttaagggaag
    13321 aggatgaaat gctgaaaatt caggataagg caaagcattt ggaatccctt ttacaaagcg
    13381 ctgacaatga agagcaaata gcagccattt tatatgaaat atcggttgtc caggaggact
    13441 atagcaggca gaaagaacgc cttcagagtt taaaaaatga cgagttgcaa ttagaacaac
    13501 aaatcaaaaa attgggtaca atgaaagttg tggacaggac tatcgcggtt gctttgtctg
    13561 cgcttactat cgccgcttta gctgtgactt tatttttccc gccggttgga ggaggcatgt
    13621 tggctgggat tgccattttt ggcggagcgt atttcttggc tagagtaagc atgcctttaa
    13681 ttaaagcaac agctgtctgg ctgtacaata aattaaaatc gaccccaagt gagagttctg
    13741 aatcacagga tatagataat aaaccgactt taagtaatga gaaagaagaa aaaaaagaag
    13801 attctttgca aatcgataac gagtttgttc taccaccaat agatgctgag gcgtcaattt
    13861 ctgaggattc aaaggagtat caacctgtgc atgaatcaac tgaggatgta ctggtgaggt
    13921 tatcagtaaa tatcgacagg ctcaaggaaa aggtaagaat ggaaaaaaaa gaggaaatag
    13981 aggaagagtc cccagaggag cttcagcatt tttcatctct aactccctcc ccaagaacat
    14041 tgggatccag aaaactatca gcaaaagaga gtgatgatga gaatggagaa agcgagtcag
    14101 atatagaaca tcctgatgtc cctaaaattt aaatctgcat tgcaaaaaac aagaatttcc
    14161 tttaagttaa aaaaaattac tatcaaggaa tgaaaattca tgctgaattt atttcgtgag
    14221 caacctcgtg catttcacat gatttttatg ttggaattat gggagcgatt tgggttctat
    14281 acagttcagg gaattcttac tctttatttt attcgctacc tgggttatag cgatttagag
    14341 tcttactata cctttggtgc ttattctgcg ctggtatatg gaatggtggt tattggcggc
    14401 tatttgggag acagaattct aggaaccaag cgaaccatcg ttctcggtct tgttgtgctg
    14461 gccatgggtt acttttctct tgcagttact gataagcaac atgtatttct tgcattagga
    14521 ttgatttgtg taggtaatgg gctattcaaa gcgaatccat cgaatttgtt atccaaatgt
    14581 tatgatgaca atgatccacg attacatggt ggttttaccc tttattatat ggctattaat
    14641 ctgggcagta tggttgcatt attcgtcgga ccagcagtat caagcgctta tggttactct
    14701 tatgcttaca tgattagtgc aataggtttg attttgggta ttgtgaatta ttggtatcaa
    14761 cgacaacatg tgtctcatgt caatacaaaa gcggatcttc gtaaaataac agtctttcac
    14821 tggggaataa taatagcggg tgttttaata cttactggaa tatcagctta tttattacaa
    14881 catgtcatgc ttgccaaaaa tctgttgtgg ggtattactc tcttggtagt gtctgtttat
    14941 atctatttaa tgcgcaaaca ggctcaggct tcttttatga gaatgttagt cgctttggtt
    15001 ttaatgattg aagcagtcat attttttact ttataccaac agatgcctac ttcattaaat
    15061 ttgttcgctg taaataatgt aatacctact ttcctgggca tcactattga tcctcaaagc
    15121 tttcaagctc tcaacccaat atggattgta ttaatgagtc ccgtattagc ttatttctat
    15181 agaaaattgc atcaacataa tattttattt tctatacctt ataaatttgc attcggcatg
    15241 accatgtgtg cagtgagctt tttaatgctg tattttgctc gttattttta tgttggaaat
    15301 ggcatggttt cttcctggtg gatgattttc agttatttat tccaaagtct tggcgagtta
    15361 ttagtatcag ctcttggtgt tgcaatggtt gctgaattgg tgccggccac taatgctggg
    15421 tttgttatgg ggatgtggtt tttaacttcc gcagtggcag ggtttattgg tgctactgtc
    15481 gcgtctttta cagcacttcc agagaacatc aagccagggg ttgagtcttt attgatttat
    15541 tccgatgtat ttgcctgtat tggtcttgtt actctattga ttgcagttat tatgtggtta
    15601 gtatcacctc ggctaagccg ctatattaca gtcccttcct tggaagggga taaggatata
    15661 gaaacagtag aatactctac gacctatatc cccagtcact aggtttgttt atttcccttt
    15721 tcggcaattg agttggtttt aatatcacca attgccgttt tttatttttg tcctattaca
    15781 tatctcttaa tgttaattta ataaaaaatg tataaaatat gtacttgcaa tcaatctttc
    15841 cctaatcatt accgggttaa ggttttcagg ttctgtgagt taatttgctt ggagtataaa
    15901 gtggggcttg aaaaaaaaat taagctaagc cagcgttctc tggaattatt aaacgattac
    15961 ttaaatttgg gtgggtctgg gccttccaat ttagtgaatg ggagttcgtc gattgatttt
    16021 tcatccttaa ctcaactact tgccagtaat ctgtccaaat catccaaaat ttcaaaaaca
    16081 ataaaaaaga aaagagaatt tatagaggca gctttgtatg ccgagttgtt acgtgactta
    16141 agtacttcat tgaatggatc cgatgaggaa atcaagcctg cagaaaaaga acctcctgtc
    16201 tctaataagt tgaaattctg gcttttggca atagctggga cattattagc cgcatgcgag
    16261 gggtttgaca gcattacaac catgatggct gtattatctt ttcctgcctg ggttgtcctt
    16321 tgtgcaggta ttttattttc cgctttatct gtcgttgtat tttatgggtt tgatttagtc
    16381 caggtttctc aaaacctgga aataaaatta actgatactc ccaagctatt ggatttatac
    16441 ctgtcacaaa tggaagaaat taaggcgatt agaaaaaaaa ttgacaatta ttctcttgct
    16501 tctatgtcaa tggatgagtt gaatgagttg catttaatcc ttgttatgct gcaagaccgt
    16561 gtcaactctt tagtaaaagc aggcaaaaaa tttgatttgg cactgaatag ccccaaaatg
    16621 gatgtcgcta aaacaattgt ttctggagta tctggaatac tcttttttgg tggaggtttt
    16681 tttgccgggc aatcagtagc gacattcatg ctggctctgg ttatgacttc tgttacacct
    16741 acattttggc cagttttatt atttagtgtg attgtcggct tggctgcttt tagtttgtac
    16801 tggtatgtgc aacgcgtagg agtaaataaa ctggttagcg aatggtttgg tttggatgag
    16861 gataaaatag aaaaattatg tggaaagtcc aagatggaca aagaattgaa aaagctaaac
    16921 aaccttgatg aaaaggtaat gactacttcg gatttgctct cgaaagtggc taatttggag
    16981 agtaaactaa tagatatcgg gaaacaaatg aaggagatga gcgcgcacca gactcctgaa
    17041 aagaaattag agggtgctgc tggattaaag gcaagttcta atgtgtattc ctttcacttt
    17101 aattcctcaa atcgggatca ggagagcgtt tacggtgaga gggtccttga tgatgctgtt
    17161 tctgattatg gtaggggtca gaataatgat tcaatccata gcctatgtta gccaaatgca
    17221 aaggcatata gattaacaga gagtttcctt gcaaataatc cggattggct gacatataaa
    17281 actcttttaa ttcactgctc tcttcattaa ttcttgaaag ctgacttctt aaaggaacca
    17341 gtggggaatt gacaaccata ggatgattat tggttgaaat ccagccgttt tgttttttaa
    17401 gggcgtttag aacggtcaat atttgatctg gaaccctttg taatggttta ggatatatta
    17461 ggtcactcaa ttgataaata aattcgtaag ttaacgggtt gttggcaaat gctatgtaaa
    17521 ccaattcctc gtgaggattg gctaacttgt attttatcgc ctgagtgagg ccggcacttt
    17581 taatagatgc gtgaatttgg tattcaggat ttagatacaa ataagcgata taacaagtca
    17641 cttgtttttt tcccagatca acagattgtc tgcagttacg gctgaatccg gctatttctt
    17701 tattttctcc atagagaacc gttaattccc catacaaatc aggctgtatg atgaaacgta
    17761 taaattcttc ttggctggcc aggtgataat acttggagag gaagcgatat aatttattta
    17821 ttaattttaa ggagattttt ttcttttctt catctacaac ataagaatgg atcgctttaa
    17881 catcttgtct gttcattgct ctctccatga aacatttttt tcgctttaat ggctcgctgt
    17941 ataatttatc cataaattat ctaagtaata gcgaatagca gataaaaaat tgtgcaactc
    18001 tttaaatata gactcgtttt catttttgtc aaattgaaga ataacaattg tcgtaactcc
    18061 atgaaagggt catctcagta actctgtgga taggcaatat atgggaaatg ataaaagaat
    18121 tacggtggat cctcatcgag gatcttaaat aaatttataa aggagagtca ataagcactt
    18181 atattttcaa aatattttta acaaaaggta tggtgatttt acgatgagcc tcccaggatg
    18241 catcatctag tcggttgagg atctcatata aatcatgcat gtttcgagag caacggttta
    18301 ataaaaaatg tccgacactc tcaggaagtt caaatccgcg ctttgctgcg cgcattttga
    18361 gtgtgtttat tttttcgtca tcatttaatt cattcaactg aataactaat ccccaactta
    18421 gtctggagcg taaatccgca agtttaatgt cacattttat tgggggttga ttacctgata
    18481 taattaaaag cgaacgttcg gagtccttaa tcttattata taaatggaat aaagcttctt
    18541 cccaggccga atcgttagcg atggtattaa tatcatcgat acaaattaaa gtttgatctt
    18601 ctaatccttc aatgctttgt ggtccccatt cttttaaaaa agctaaaggg aggtaaatgg
    18661 ctgattgggt taagttaatc gcttggcaac aagcttgcag taaatgtgat ttgccgcttc
    18721 ctttagggcc ccaaagatag agcaacctgt ctgctttaaa agaaagcatt tcaattaatt
    18781 gctgctgcaa caaggtatta ttaccccaat tgaagtcgtc cagggttgct tcatcattca
    18841 attttattgc cagggccagc tgtttattca ttttaacgat tctttggggg accaggcttt
    18901 tctgctccag gtccaaacat aatcaaggta ggaagtaacg gtggtaaagg cagttaaata
    18961 gatgagtata tcaactaaat cataggggaa tctaaagtaa gctaattcaa ataaacataa
    19021 agtgactaaa gttaactgga agccggtatt gagtttactg agtcgagtgg gctcaaaatc
    19081 cagtttgcgc tgtataaagt aataccaagc taatactccc attgaaatag tgaagtctct
    19141 aaaaaaaaca agaagcacta accaccaggg caaggagctt aagagagcta aagaaatgaa
    19201 gctggaggca actaacaatt tatctgcaag agggtcaaca aaggagccaa aaaaactttg
    19261 ccattgaaaa tgcctggcta accaaccatc caaaccatca gttaatcctg cggcgataaa
    19321 tatataaaag gccttaacgt actcatgatg aaataaatac attagaaaag gagctattaa
    19381 aactaatctt agcaatgtta aggtattggg aatatgtttt agtatcatct gttatacgaa
    19441 aattagttcc tcctcttttt ttatttatcg ctgaggaggg aatatattgt gccaacaata
    19501 ctctattatt tgagtgtttc aaatggcgta tgtgaattag tttatacaat accattgtct
    19561 ctgtcacctt attgagaaat caattgggat ttattgactc atagtatccg catcccgtcc
    19621 tcgagaagtt ctcatatttt aagattgcaa caagagcgca tggggttttt cgtaagtcca
    19681 gtagagctta gatctcggat aatgggttgt attcctttat tgtaatattt tgattgagtt
    19741 tggaatgcag acttaataac aattcaggtt gatgctccca gttgtcatgg agcaatagat
    19801 gttgttgcca acttagaata cgcggatcaa tgtctttgga taagtcttta aatacagttt
    19861 tcatgacttg gcgagttaat ccccataagg ttcttgaagg aatctgatac tcattacata
    19921 aacagccaat caagtgatgt agatgatttt ttaatgttcc ctggatgaaa agggttctaa
    19981 cctgattaag atcttttgta tagatggata gagagtctgg agcgttgggt ttttgaacgt
    20041 ttttaaacaa ttcatgattg catagcttaa ggttgtttgg ctcttttata attatcccct
    20101 gaggtttatt atcatcgaag atgactaaaa tattgtgttg ctccacttct agtgctaatc
    20161 catattttag caatagatgc aattgtccaa ataatatctt ataacaatat tcagtgaaat
    20221 aagtcgttgg atgcagttcg ctagctttaa taatttcaat gagtaatggt gtattgctac
    20281 aaggtgaatc agttaatagg gccggtaatg gaactgctct ttgatccggt cttattttgt
    20341 ggagtgggtt ttgatataag ccaaacaata attttactcg attgcattca gagagagttt
    20401 gatcataggc gctcatgctc tccaggtttt tacatataaa caaggtattt tggtaatagt
    20461 ttgtttttgc taacagggaa tcgatccatc tggtaagtaa taccatgtca tcagaattat
    20521 tgagttttcc agccattgag gtgcttaaag tggtagccag tttcagtgta cattgagtgg
    20581 attgtgttgg catcataata tcaggtgata aagaaggcat tagtgtttgg tgatgaggta
    20641 acaagatgag ggatttatta tcaattaaag aagcaaacgc catttgtaat tgattcctcc
    20701 agtgccaagg gtgaacagga attggataat aatcatcagg gttgatgtgg ctaaataaca
    20761 atttttcacg ccaataaaaa tattctttgg gaaattcttg agaaatttgg ttggcataat
    20821 caatttttgg gtttattgac tgaatttttg ttttatttaa tgcacaccaa tgtacactga
    20881 ctttggcctg gaattcggga gagttttgga gtacttctct tcgcgtaaat cctgtttttg
    20941 cgctgaatgc cgggtgatag ggatgcctca aactgcccca ttgttccaaa aataatagcg
    21001 tttgatacgg tgatagttca ttatttatcc atgtccacag actcttgtag tgcatggctt
    21061 catttcttaa ttgggtgttc cagttttgcc tgtaggcgta agccaaagcc tcattagcaa
    21121 tcgattcatc taatgcttcc ctgatttttc gccattgaaa aaacttgctt tctggcattg
    21181 aatttttcag ttttatctgc aattggtcaa tgaaatcatg gacatggtga cttgcaatgg
    21241 ctgattgaat taatccttcc ttaatggagg catgctgtaa acggttcagg gtatttttat
    21301 gagctaaggt aataaaataa tccacactat tttgtggtag tcctatgcca atttcaaata
    21361 gtaaaaagcg tagttgatgg ctgagttcat gaaaattacc gtacgccaag gccattatta
    21421 tctcctgaag gattgttttg ttctaaatta aatataaatg ataatcattc tcatttgcaa
    21481 gaggtggggg gcaaataaaa tattataaat tgataa
//
LOCUS       DASLOE010000038        21185 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.038, whole genome shotgun sequence.
ACCESSION   DASLOE010000038 DASLOE010000000
VERSION     DASLOE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 21185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21185
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.038"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            186..2441
                     /gene="parC"
                     /locus_tag="U6309_001142"
     CDS             186..2441
                     /gene="parC"
                     /locus_tag="U6309_001142"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEN5504595.1"
                     /translation="MKKIPNMNEVTEKKPLTEFTEKAYLDYSMYVILDRALPSIADGL
                     KPVQRRIVYAMSELGLKATAKYKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYR
                     YPFVDGQGNWGSPDDPKSFAAMRYTEARLSHYADVLLGELLQGTVDWVDNFDGTLQEP
                     ALLPARLPNILLNGATGIAVGMASDILPHNLTEVANACIHLLDNPQADIKTICEYIKG
                     PDFPTEAEIISPSETIRAIYESGNGSIKMRAIYTQEKQNIVITALPYQVSGARVMEQI
                     ALQMQQKKLPMVEDLRDESDHEHPTRLVIIPRSNRVDIESLMSHLFATTDLERSYRVN
                     FNMIGLDGKPRVKGLLTILTEWLEFRLTTVKRRLQFRLEKVLERIHILEGLLIAYLNI
                     DEVIAIIREHEQPKQGLISRFNLTERQAEAILEMKLRHLAKLEEIKLRGELDELSDER
                     DTLQKTLASHSLLKELVKEEIIKDRDEFGDPRRSPIITREDSQALKEEDILPNEPITV
                     VLSKNGWVRAAKGHDVDGKELSYKAGDEFKAQTLARTNQQVLFFDGEGKVYSLPGHAL
                     PSARGQGEPLTGKLNPAEGVLFEAIVGGEPEQKVLLATDSGYGFIAKIEDLYVKNRNG
                     KACIKLSENSHILPPRLIPNREQLFVACATNVGRLLIFSLEELPELSRGKGNKLISIP
                     AAKAASREEYIVDMQVLTTNDSLTVYAGKRHFTLKGEDLTHYKGERGRRGNRLPRGLQ
                     NVSQLHVTQSE"
     gene            complement(2548..3402)
                     /locus_tag="U6309_001143"
     CDS             complement(2548..3402)
                     /locus_tag="U6309_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEN5504596.1"
                     /translation="MNFNIIGAGRLGKNLGIAMSVHQIASLNSICNSNLASARIACNE
                     IGFGNAVGKISDLSEADITWITCRDDSILDVVSILDNTEILKKGSLVIHCSGALNSNV
                     LLPLKKQGCYIASAHPLKAFRTNSLDSNTFKQVHCVIEGDYEACRWLRFAFEKLEASI
                     IAIQPEMKVIYHTAATIASNYLVTLASYSEELFLKAGIQQAQSRKMICDLMYSNILNL
                     NQVDSAKSALTGPLTRGDMQTISLHLEAIENSEIKRLYKSMALATLPLLDLSLDRKET
                     LRKTLEKE"
     gene            3652..4213
                     /locus_tag="U6309_001144"
                     /pseudo
     CDS             3652..4213
                     /locus_tag="U6309_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947875.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family peptidase"
     gene            4846..6375
                     /locus_tag="U6309_001145"
     CDS             4846..6375
                     /locus_tag="U6309_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504597.1"
                     /translation="MSITAQELVKQYKLRLTPAMENDLLSEESRLKKELEAVPFNSEE
                     ALYKSILQMIIVFYEENTLEENRYLLQDHELIKQLSALMWDDIQIKLIPFLIQKNFTL
                     SEIKELLFDDAYYRSLHVLVDFGLTQDIPELLAHQEKREQLKFINTLANDHCRKLCLI
                     FWVKGSLSIKEIQDIVNATSHYPMLAETLIALDKTKTISIKQLKKLALDPKKHQQESI
                     LYHYSEQFKAYNLRKSDLSQLNLDDLDALGKSFKVLKEAGIANDYAYRLVLKNNKTGQ
                     LLRLFLPGLAKIESLSHRKALIELLYIGAQKGVVTQGKALLQIKDSNLLALSRALRER
                     FICVQQMQDLGFKKEIIAFTGEENNINSSRFRHVIMRVEEKCKDIHERLRKSSLDKDK
                     VGNWQRADEKYRQTLYSIAYDGITKSGVDLHIKMKSAEKEILSIVDPEIKSIIHKVLV
                     VIANIIITALTLGFANDLKESATGNYWFFNQSPSGEVIRALNKEVLTTIDSPELITIL
                     P"
     gene            6638..9253
                     /locus_tag="U6309_001146"
     CDS             6638..9253
                     /locus_tag="U6309_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2975 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504598.1"
                     /translation="MGLPKKALKESQLQFLTAGTAVSDSSHQTYKVSFIENGVIKNAF
                     YKKLDPKNHYPELLAKISVAVSLFKRIFQGRRSAEERLVFDDEERLVGTLSISVDGFK
                     GFNFHKESVPQESSAKEQVIPSTRTLIEKSFMEILLGRWFLDDDDGHPHNLSLAGDID
                     FDMFFYWFTIYMKEPRPAIGIPKTRVNLTVRDWEGFPNVKDSKPFHWPTYKNPGQETL
                     PTVLPVQDKLVNLILEKTYPDPGQFEQLAHEPVAQEQKFAAALKILLTYQPEMIRKRL
                     TELFGEMTLNYTSLDETDVALRNQYEKTFPHLCNENTNIKPFVDFIMNLYQMHYDNLY
                     RVVVFYMGCENNGYGVPLPATNSALYHKPSFYKDIVEWARTQNITIFSKDDSSIKFDE
                     DELRRRYHQVWRDAYAPTFRDLLHDSYSLTNKLLQQVSTFHVVLDEVEGKKPTDDTLT
                     NAWELFGTMPELSLEKITPLISVDKDSKLRTALILLVEFTTQFHAVAKTYYQKDRKDL
                     TEEDNLEFSEQLVQLYTNYNLKIRQSLAHTSTLAGEFNRIAVGLKQYTERANFQLHLT
                     TTDEQMKEATVATTPKEILPHTHEDVIRQFNDSLFLWAKNLRPEDLSHHISEIIDKYY
                     APTIELLSKRHRAQPVKEYLQASVNESGENRLAYILSAGEGDTGALNTLLIQHLTPYM
                     LQTYPLLSIRNAVKEGNFDKDLEIFTKAAVDFAKHDRRFIHLYNVEGKSLFFKTMYEW
                     IDELPATKFKGLLESALKDYEGKLWWSTSRRSEVEGYCTKFSQAKIVAMTFLNGKDSS
                     SLNDVLFDKIIAAIQKDINKNKEKLKIPGFRLINCYNAKEHRADYFKEVKNYAEPISH
                     RQETTLNSNVTSLVV"
     gene            9346..10197
                     /gene="psd"
                     /locus_tag="U6309_001147"
     CDS             9346..10197
                     /gene="psd"
                     /locus_tag="U6309_001147"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842959.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEN5504599.1"
                     /translation="MFRDVLKTLPQYLIPKHGITALAGYFADVKNPRLKNFLIQNFIR
                     KFDVDMSEALIEDPKSYDCFNDFFIRHLKPECRPLSQSDVICPVDGCISEIGKIERGQ
                     LLQAKGKYYSVQELLACDGQLAEQFVQGQFATLYLSPKDYHRVHMPIDAELVSMTYIP
                     GALFSVQPATTRVVPKLFARNERLAIFFKTKIGPMVMVMVGATIVGAIGTSWHGDVKR
                     SKELERFDYSEQFLDKIISQGSEMGYFKLGSTVVLLFANGEKIQWDKELLAGSKIQLG
                     KPMAIIT"
     gene            complement(10202..10288)
                     /locus_tag="U6309_001148"
     tRNA            complement(10202..10288)
                     /locus_tag="U6309_001148"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10252..10254),aa:Leu,seq:cag)
     gene            complement(10371..11339)
                     /locus_tag="U6309_001149"
     CDS             complement(10371..11339)
                     /locus_tag="U6309_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEN5504600.1"
                     /translation="MSIITTNLNRAIHDLQQGKPVAIPTETVYGLAAPINNNAAIRAV
                     FEMKGRPLNHPLIVHIASNWDLHKLVEEVPEYAHQLASKFWPGPLTLVFYCKKDQINP
                     LITGGQSTIAIRCPDHPLTQKLLQKLNIPVVAPSANPFGKISPTTALHVKQSFNDAEL
                     TILDGGRCRVGIESTIVDATNPVSYQILRHGIIDEKTIAETIAIGCSTQKNNIRVPGK
                     LDSHYQPQKTLYYFDQYETLSEFCQKRKGTVYVIANRQPPGVETNLFHPLGMHPEKSA
                     YDLYYQLRLADTSDAICIAIELPPNTSEWQGVRERILKAGFPYSQQ"
     gene            complement(11569..13239)
                     /locus_tag="U6309_001150"
     CDS             complement(11569..13239)
                     /locus_tag="U6309_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEN5504601.1"
                     /translation="MAIKVTKRGMDLLRDPILNKGTAFSLQERDEFALHGLIPTTVET
                     LEQQVIRCLDAYSAKENPLEKHIYLRALQDRNEVLFYRFIIDNLVDILPIIYTPVVGQ
                     ACEMFSHIYRQPRGVFLSYPERDKLDSIIQNIASTRSIKVIVVTDGERILGLGDQGAG
                     GLGIPIGKLSLYTSCGGIHPSNTLPIILDVGTNNKERLDDPEYIGWRHARISGKEYDD
                     FVDQFVQSIKRHMPHVLLQFEDFAQQHAYPLLERYKNQLCTFNDDIQGTASVAVAAIL
                     AATRVTNTPLKEHRVALLGAGSAGCGISEQLVHAMMNQGLSEEEARSRFYLVDRYGLL
                     HDEMTDLLPFQKGFVRSSTSLQNWKLEKKGEITLTDVINNAQPTILLGVSGQPNQFKE
                     AMIKTMLSYCERPIIFPLSNPTSRAEAIPQDLLNWTAGKALIATGSPFEPVVINGHKI
                     EIAQCNNSYIFPGVGLGVVAGQAKRVTDLMMMAAAVALSELAPAIRTGEGRLLPELNS
                     IREVSQHIARAVILQGIKEGHIEPMNNNKIDDSIKRTMWTPQYEPYVL"
     gene            13491..14273
                     /locus_tag="U6309_001151"
     CDS             13491..14273
                     /locus_tag="U6309_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN5504602.1"
                     /translation="MLLKCIEKIVDGYFAVIPRHTPEFENINGALIIAHRGAHNNARG
                     IIENTLEAFQNAKETGCWGIELDVHLTKDKVFVVNHDPTLNRLWNKDIAIADLNFSEL
                     RALECRIPSLSEIVSQYGNSMHLFIELKTAIEEEEILVQTLQHLTPCKDFHLLTLQES
                     TFKKLCSFPRQSLLLVAVHNNVKKFCELSIKEGYGGVLGNYLLLTNRALKYLQDANQH
                     FGVGFVDSKNSLYRELNRGVKWLFTNEAININQYLNDLKNSM"
     gene            14425..15192
                     /locus_tag="U6309_001152"
     CDS             14425..15192
                     /locus_tag="U6309_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN5504603.1"
                     /translation="MTLKITFLGSGSSFAIGPENYQSNVLLELENDTLLIDAGTDIKY
                     SLRDQGLTYKDIKNVYISHLHYDHCGGLEWFALITYFDPNHSHKPCLIASDKIINELW
                     DKSLSGGLSTLPHERANLSAFFDVKPVKQYEGFVWQSIRFKLVQTVHYYSEYELMPSF
                     GLIFNYNKSRILFTSDTQSCPVQLISFYEESDIIFHDCETTESKSGVHAHYSELVQLP
                     PHLKKKMWLYHYNPGKLPNAKKDGFLGFVAKGQSFVF"
     gene            complement(15245..16414)
                     /locus_tag="U6309_001153"
     CDS             complement(15245..16414)
                     /locus_tag="U6309_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEN5504604.1"
                     /translation="MSLITSYSPMPVTFTHGEGVWLYDEQGKKYLDGLSGIAVCGLGH
                     AHPDVTRTIQEQAARLIHTSNVFHIKEQELLAAKLTSMARMEQVFFANSGAEANEAAI
                     KLTRLFGHKKGIETPSIIVMEKAFHGRTMATLTASGSRKVQAGFEPLVPGFIRAPFND
                     LDAIHTIAANREDVVAVMVEPIQGEGGIYPAEEGYLRALANLCEQNDWMLILDEIQTG
                     NGRTGKLYSCMHYDIQPDILTTAKGLGNGMPIGACLMSKRAKDLFKPGNHGSTFGGNP
                     LACATALTVLEVIERDKLCERVTKNSALLMDKLISNLGEHPHVKAIRGKGYMIGIELD
                     RPANDMRVLGLENQVLFNITAETVVRLLPPLIISEDEIDELVDRITRTINQFTNQ"
     gene            complement(16473..17051)
                     /gene="sodB"
                     /locus_tag="U6309_001154"
     CDS             complement(16473..17051)
                     /gene="sodB"
                     /locus_tag="U6309_001154"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HEN5504605.1"
                     /translation="MTFTLPQLPYALDALAPHVSKETLEYHYGKHHNTYVTNLNKLIP
                     GTEFESMTLEEIIMKAKGGIFNNAAQVWNHTFYWHSMSPNGGGEPKGRLAEAINKSFG
                     SFAAFKEQFSQTAATTFGSGWAWLVQDQSGALKIISTSNAGTPMTEGLNALLTCDVWE
                     HAYYIDYRNRRPDYIEAFWSLVNWDFASSNLK"
     gene            17379..17699
                     /gene="grxD"
                     /locus_tag="U6309_001155"
     CDS             17379..17699
                     /gene="grxD"
                     /locus_tag="U6309_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEN5504606.1"
                     /translation="MVDTLEKIKKQIAENAIMLYMKGTPKMPQCGFSARAVQCIEACG
                     VDFAYVDILANPDIRQVLPQFSDWPTFPQLYVKGELIGGSDIIAEMFQQGELEPMLRD
                     AVAA"
     gene            17752..18357
                     /locus_tag="U6309_001156"
     CDS             17752..18357
                     /locus_tag="U6309_001156"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin C"
                     /protein_id="HEN5504607.1"
                     /translation="MSVLVGRKAPDFTVAAVMGNGEIVDKFNLHEHLKGKYGLVFFYP
                     LDFTFVCPSELIALDHRIEEFKRRNVEVVAVSIDSHFTHNAYRNTPVKNGGIGPVRFT
                     LAADMTHSICQSYGVEHPVAGVAFRGAFVIDTNGMVRSQIVNDLPIGRNIDEILRIID
                     AVQFFEENGEVCPAGWQKGQAGMKASPKGVAEYLSEHSESL"
     gene            complement(18483..19106)
                     /gene="rnt"
                     /locus_tag="U6309_001157"
     CDS             complement(18483..19106)
                     /gene="rnt"
                     /locus_tag="U6309_001157"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEN5504608.1"
                     /translation="MVSRNNILSNTIKNRFRGFLPVVVDVETAGIDPQKNALLEMCIV
                     LLDMDEQGLLKKESSYFEHILPFPGAELDQKSLEFNQIDPYQPLRFAVDEQTALERLF
                     KPIHDLLKKTRCQRAVLVGHNAWFDLLFIKAAIKRTGIKSPFHAFTCFDTATLAGLIF
                     GETVLAKAVQAAGLHFDSQEAHSAIYDAEKTADLFCLMINRWRMLNI"
     gene            complement(19090..20127)
                     /gene="pyrC"
                     /locus_tag="U6309_001158"
     CDS             complement(19090..20127)
                     /gene="pyrC"
                     /locus_tag="U6309_001158"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEN5504609.1"
                     /translation="MQTLIINRPDDWHLHLRDGEFLTHTVQASAQHFARALVMPNLKP
                     ALTNLPAIESYRNRIISAIPKQNSFIPYMTFYLNESVKPEELQQAASLSYIVGAKLYP
                     AGATTNSEEGAKSLKALYPLFETLQTSNLVLQIHGEVTHSDIFEREALFIDEYLKPIA
                     KHFPKLRIVLEHISTKAAVDYVSEGPANLTATITPHHLLYNRNKLLAGGIRPHYYCLP
                     ILKHEKDQKALQVAATSGNPKFFAGTDSAPHAINAKENACGCAGIYSAPYAVALYTQI
                     FDELNQLKKLNDFLSRFGAEFYQLPINQQQLELIKSPQTIPNSMSFGSTHVVPIAAGD
                     TINWSINGFSQ"
     gene            20197..21081
                     /locus_tag="U6309_001159"
     CDS             20197..21081
                     /locus_tag="U6309_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEN5504610.1"
                     /translation="MISDHFICKKAKIIAMSSLFITTVIFAAPSIQYASPMGEENWRM
                     TGNPLRCGLSLTIPNYGIGYFEQYATQSPHFILRKWDQVQRALPAQVVASPPVWKPLL
                     KGSYLVTRTYIKPGEYGLFLNRQPALKLLTFLSQGYQANFSYLSEEGFRTTVILSPVR
                     FQKVYSQYQRCLGGLLPFNYEDVKESVFHFGVDSKLLSDADKVQLRRIAQYVDADVQI
                     DVIKIIGHADDSGRKGYNNAISQYRAESVKNYLIRLGVPQRKLSITWVGALQPIARND
                     TDEGRAANRRVVVTLIKK"
BASE COUNT         6537 a         3783 c         4308 g         6557 t
ORIGIN      
        1 aatgaaatta ataaatgtta atataaatca ataagttaaa cttaatccat atagataaag
       61 caaaaagaat ataaagttat acacagataa tacgatgatt ataattctaa cagcatgaat
      121 attctgttaa aattgttttg cattgtctta tacgattcac taggatgatg gatagcaatt
      181 aatatatgaa gaaaataccc aatatgaatg aagtaactga aaaaaaaccg cttactgagt
      241 ttacagaaaa agcttatctt gattattcaa tgtatgtcat tttggacaga gcattaccga
      301 gcatcgcaga tggacttaag cctgttcaac gcagaattgt ttatgctatg tctgagttgg
      361 gtttgaaagc aacagcaaaa tataaaaaat cagcgcgtac agtaggggat gttcttggta
      421 aattccatcc tcatggtgat agtgcatgct atgaggctat ggttttaatg gctcaaccct
      481 tttcatatcg ctatccattt gttgatggac aaggaaattg gggatccccg gatgatccca
      541 aatcatttgc tgcaatgagg tatacagaag caagattatc tcattatgct gatgtgttac
      601 tcggagaact gctacaagga actgtagatt gggtagataa ttttgatggc actttgcaag
      661 agcccgctct tttaccagct cgtttgccta atattttatt gaatggtgca acaggtatag
      721 ctgttggtat ggcttcagat attcttccgc acaatttgac tgaagttgct aatgcgtgta
      781 ttcatctgct ggataacccc caagcagata ttaagactat ttgtgaatat attaaaggcc
      841 ctgattttcc aacagaagca gaaatcatta gcccaagtga aacaattaga gctatatacg
      901 agtccggtaa tggctcaatt aaaatgcgag caatttatac tcaagaaaaa caaaacatcg
      961 tcataacagc tttgccttat caagtatccg gagccagggt aatggagcaa atcgcgttgc
     1021 agatgcaaca gaaaaaatta ccgatggttg aggatttacg tgatgaatca gatcacgagc
     1081 atccaacacg gttggttatt atccctcgct ctaatcgagt agatatagaa agtttaatgt
     1141 cgcatttatt tgctaccacg gatttggagc gtagctatcg agttaatttt aatatgattg
     1201 gtcttgatgg caagccaagg gttaaaggac tgttgacaat cttgactgaa tggttggagt
     1261 ttaggcttac cacagtaaaa agacgtcttc aattcagatt ggaaaaggta ctggagcgta
     1321 ttcatattct tgaaggactg cttattgcct atcttaatat agatgaagtc attgcaatca
     1381 tcagagaaca tgagcaacca aaacaaggat taatttcacg ctttaattta accgaacgac
     1441 aagctgaagc aatattggaa atgaaactgc ggcatttagc caaattggaa gagattaaat
     1501 tgcgtggtga actagatgag ctgtctgatg aacgcgatac tttacaaaaa acgctggcta
     1561 gtcattctct cttaaaagaa ctggtaaaag aagagataat caaagatcga gatgaatttg
     1621 gtgacccacg ccgttcaccc attattacaa gggaagactc tcaggcatta aaagaagagg
     1681 atattttgcc taatgagcct attacggttg tattgtctaa aaatggatgg gtcagagcgg
     1741 ccaaggggca tgatgtcgat ggtaaggaac tcagctataa agctggagat gaattcaaag
     1801 cccaaacgct ggctcgtact aatcagcaag ttttattttt tgatggagag ggaaaggtct
     1861 attcgcttcc aggacatgca ttgccatcgg cacgcggtca aggcgagcct ttgacaggaa
     1921 aattaaatcc tgctgaagga gtactgtttg aagcaattgt tggcggagag cccgaacaaa
     1981 aagtgttatt ggctaccgat tccgggtatg gatttatcgc caaaattgaa gatttatatg
     2041 taaaaaatcg taatgggaag gcatgcatca aattaagtga aaacagtcac atattgccac
     2101 caaggctaat accaaatcga gagcaattat ttgttgcttg tgcgactaat gtagggcgtt
     2161 tgctgatttt ttctcttgag gagttgcccg aattatctcg aggaaagggg aataaattga
     2221 tcagtatccc cgccgctaaa gcagcctcac gagaagagta tatagttgat atgcaagttt
     2281 taacaacgaa tgattcactg acggtttatg ccggtaagcg gcattttact ctgaaaggtg
     2341 aggatttgac tcactataag ggtgaaaggg gccggagagg aaaccggttg ccaagaggtt
     2401 tacaaaatgt ttctcaattg catgttactc aatctgagta acatggccgt tagggtctgt
     2461 atgtttcccg tgaaacacct ttaagtgatg atttgaaatc aaacagaata atgcacaggg
     2521 ctggcttatt gttcaataac ggcaatgtta ttctttctca agagtttttc tcaaggtttc
     2581 ttttctatct aatgataaat ctaacaaagg cagggtagct agcgccatgc ttttatataa
     2641 tctcttgatt tcactatttt ctatcgcttc gagatgcaag gaaatggttt gcatatctcc
     2701 tctagtcaaa ggtccggtta aagcactttt tgcgctatct acctgattca agttaaggat
     2761 attgctgtac atgaggtcgc atatcatttt tcttgattgt gcttgctgta ttcccgcttt
     2821 taaaaataac tcttcactat atgaagctaa agtcactaaa taattagaag caatcgttgc
     2881 cgcagtatga taaattacct tcatttcagg ttgaattgcg ataatgctgg cttccagctt
     2941 ctcaaaagca aacctaagcc aacgacatgc ctcataatct ccctctatca cgcaatggac
     3001 ttgcttaaac gtattggaat ccaaagaatt agtgcggaat gctttcaaag gatgcgcact
     3061 tgcaatataa caaccctgtt ttttcagagg aagaagtaca tttgaattca gagctccgct
     3121 gcagtggatc accaagctgc ctttttttaa tatctcagta ttgtccaata tcgatactac
     3181 atccaaaatg gagtcgtccc tacaggttat ccacgtaata tctgcttcag aaagatcaga
     3241 tattttacca actgcattgc caaatccgat ttcattgcag gctatcctgg cacttgctaa
     3301 atttgagtta caaatagaat ttaaagaggc aatctgatga acagacattg caatgcctaa
     3361 attcttaccc agacgcccag caccaataat attgaaattc atagtgaaac caatgtctgt
     3421 ctcagtacat tttcttatta taatggaaaa taatttattg tcttgctaaa ttatcagtct
     3481 gaattaatcg gacattagca gagtctgagg gaatcgtttg cgataagaag ttgagtataa
     3541 attgttattc cagatataaa aaataaaatt ttgtcgtaat ttgtgtcttc tgattgtttt
     3601 ttctcgaatt tgtagcagca tgaagtttcc acaaaaaatc aggatgatat tatgaaaaaa
     3661 cggattgttt cattatttgt catttcaaca gttatttcat ctgcaaacgc tgcaaaccag
     3721 gcaatcgtat ggggtgagtc aacgaaagca ttacctcaat ttgtgcaagc acatactttt
     3781 aagaaacaag gattattgca aactgtaaaa agtcagcaaa gtgattataa actggagtta
     3841 caaaataatt ctaaccatgc gatcaggcat gctcgttatc aaataaccta caaaggggtt
     3901 ccggtttggg gttatcaagt catttttcat tccaaagatg gcgccaaaga aactgtaaca
     3961 ggtatgaata ttaccggaat tgaacaggat attaattcta ctgagggaaa tttgagcccg
     4021 aatgatatag aacaaaagat tcttagaaag gtaactgaac cggttaaatt taaacattaa
     4081 aaaggttatt catatagata ggggaaataa agtcatttgg catactattc caatagtgaa
     4141 aaaaaaacat ttcaatgcac ctaattatct tattgatggc aatagcggtg agattttgaa
     4201 acaatgggat aaaacacctg ttatcgaggc atttgccgag gtgggggtcg tttgtccgat
     4261 gcataaaagc tagttttaag tagtttgact tcagttcaag ctacttgaag caacgagagt
     4321 tgctaaggaa gattgatgat tttttataga acgcctactg gataagataa ttattccagg
     4381 tattcaaagc ttaaaagacg aggcttagtt caatagactt cttgcctaac ccagtctctt
     4441 tcgttttatt gcagcgttgc aaacgtttct gcggcgctca tttgctgtac gtaatcttcg
     4501 ctccttaaaa tgtttgcgcc aaaataatta ggttatgcaa gaggccaaat aaatactggc
     4561 ttggagccac tagaggatag gtagcaaggg ctcagccaca tccgagtcaa ggtttctcta
     4621 cgttgaggag gttattcctc aaaaacagag agtgaataaa actcaattta tttttaaaaa
     4681 aaatccccat ttgctgcaaa attggttata atatttacat tttgtagttt tgatactatt
     4741 tatgtatcgt tcaaaataac caaattttga tccaaaaaat aatctttatt accctatctc
     4801 catgaaatag ggttaattag taaatgaaca agaggttaac ttccaatgag tattacagcc
     4861 caggagttgg taaaacaata caagcttcgt ttgactcccg ccatggaaaa cgatttattg
     4921 tcggaagaaa gcagattaaa gaaggagctt gaagcggttc cttttaactc tgaggaagct
     4981 ctgtataaaa gtattttgca aatgattatt gtattttatg aagaaaatac tttggaagaa
     5041 aatcgttatt tattgcaaga tcatgagtta ataaagcaat taagcgcatt gatgtgggat
     5101 gatatccaaa tcaagttaat tccttttcta attcagaaaa atttcacttt aagtgagatt
     5161 aaagagctct tatttgacga tgcgtattat cgttcgcttc atgtccttgt cgattttggt
     5221 ttaactcaag atatccctga attattagcc catcaggaaa aaagagagca actaaaattc
     5281 attaatactt tagcaaatga tcattgcaga aaactatgtc ttatattttg ggttaagggg
     5341 agtttatcga tcaaagaaat acaagacatt gttaatgcta ccagccatta cccaatgctg
     5401 gctgaaacat tgatagcatt agataaaacc aaaaccattt ctataaaaca gctaaaaaaa
     5461 ctggctctgg atcccaaaaa acatcaacag gaatctattt tatatcatta ctctgaacaa
     5521 tttaaagcct acaatttgcg taaatccgat ttatcccaat taaatttgga tgatctggat
     5581 gctttaggaa aatcttttaa agtcttgaaa gaagcgggga ttgccaatga ttatgcttat
     5641 cgtttggtac taaaaaataa taaaacaggg caattgcttc gcttgttttt gccagggctt
     5701 gctaaaatcg aatcgctctc tcatagaaaa gctttaattg aacttttata tataggagcc
     5761 caaaaagggg ttgttactca gggaaaggcg cttttacaaa ttaaagacag caatttattg
     5821 gctttgtcac gagcgttgcg cgaacgattt atttgtgtcc agcagatgca agatttagga
     5881 tttaagaaag agatcattgc attcacaggc gaagaaaaca atatcaactc cagccgtttc
     5941 aggcatgtca ttatgcgggt agaagaaaaa tgcaaggata tacatgaaag attacgaaaa
     6001 tcatctcttg ataaagataa agttggaaat tggcagcgtg ctgatgaaaa ataccggcaa
     6061 acactctaca gcattgccta tgatggcata acaaaatcag gtgttgatct gcacattaaa
     6121 atgaaaagtg cggaaaagga aatattgagt attgttgatc ctgaaataaa gtcaataata
     6181 cataaagttt tagtcgttat agctaatatt attattacgg cattgacctt gggttttgct
     6241 aacgatttaa aagaaagtgc cactggcaat tattggtttt ttaaccaaag tccatctgga
     6301 gaagtcataa gagccttaaa taaagaagta ttaactacta ttgattctcc ggagttaatt
     6361 acaattttgc cctaatcata gggcttctgt taacaaaccc ttattgcttt gggcaatata
     6421 agacaaccct gaatgtttaa ttaaaatgat gatttcgagt tcgtcatcat gacaaataaa
     6481 caagcaaatt ttcatttata actcattatg tgtgccagat gtctagtttg tttctagtca
     6541 agtgtaattt tgtatgaaaa ttatacttga acttcttgac tatttctgat taatatgggg
     6601 cataggatgg attgatctga tttaaaggat ttgatatatg ggtttgccca aaaaagcact
     6661 gaaagagagc caactgcagt ttttaacagc ggggactgcg gtttcggata gctctcatca
     6721 aacatacaag gtcagtttta ttgaaaatgg cgttattaaa aatgcctttt ataaaaaact
     6781 ggatcctaaa aatcattatc cggaactttt agcgaaaata agtgtcgctg tctctttatt
     6841 taaaagaatt tttcagggaa gaaggtcggc agaagaacgt cttgtatttg atgatgaaga
     6901 gaggctggta ggtactttat ctatcagtgt tgacggattt aaagggttta atttccataa
     6961 agagtcagtg cctcaggagt cttccgccaa agaacaggtg attcccagca caagaacctt
     7021 aattgaaaaa agtttcatgg aaatccttct gggaaggtgg tttcttgatg atgatgatgg
     7081 tcacccgcat aatctaagtt tggccgggga tattgatttt gatatgtttt tttattggtt
     7141 taccatctat atgaaagagc ccaggccagc tataggtata cctaaaacaa gggttaattt
     7201 gactgtgcgc gattgggaag gatttcctaa tgtcaaagac tcaaagcctt ttcattggcc
     7261 aacatataaa aatccggggc aggaaacttt gccaacggtt ttgcctgttc aagataagct
     7321 ggttaattta attcttgaaa agacgtaccc ggatcctggt caatttgagc aactcgctca
     7381 tgagccagtg gctcaggagc aaaaatttgc tgccgcgttg aaaatattgt taacttatca
     7441 gccggagatg ataagaaagc gtttgaccga gctttttggt gagatgacgc taaactacac
     7501 ttctctagat gaaacggatg tcgccctgcg caatcagtac gagaaaacat ttcctcattt
     7561 atgcaatgaa aacaccaata taaaaccatt cgtcgatttc ataatgaatt tatatcaaat
     7621 gcattatgat aatttgtatc gagtagtggt tttttatatg gggtgtgaaa ataatgggta
     7681 cggagttccg ttgcctgcaa cgaattcagc gctctaccac aaaccttctt tctataaaga
     7741 catagtcgaa tgggcaagga ctcaaaatat tacgattttt agcaaggacg acagcagcat
     7801 caaatttgat gaagatgagt tgcgaagaag atatcatcag gtgtggcgag atgcttatgc
     7861 gcctacattc agagatctgt tgcatgactc ctatagcctg actaataaac tattgcagca
     7921 agtgtctaca tttcacgttg ttctggatga agtagaaggc aaaaagccaa ccgatgacac
     7981 cttaaccaat gcctgggagt tgtttggcac catgcctgag ttatcactcg agaaaattac
     8041 cccattaatt agtgtagata aagacagcaa actaagaaca gcgttaatat tgcttgttga
     8101 atttacaact caatttcatg cagtagctaa gacttattat cagaaagacc gaaaggattt
     8161 aacggaggag gacaatcttg agttctccga gcaattggta cagttgtata cgaattataa
     8221 cctgaaaatt cgtcaaagtt tggcacatac aagcactctt gccggtgaat ttaatcgcat
     8281 agccgttggg ctaaaacaat acacagagcg ggccaatttc caattacatt taacgacaac
     8341 agatgagcag atgaaggagg caactgtagc cacgaccccc aaagaaatat tgccgcatac
     8401 gcatgaagac gtcattaggc agtttaatga ttctttattc ctttgggcca agaacttaag
     8461 acctgaagat ttatctcatc atatctctga aataattgat aaatattatg ccccaactat
     8521 agaactgtta tcaaagcgtc atagagcaca acctgttaaa gaatatttac aagccagcgt
     8581 aaatgaaagt ggtgaaaaca gattggctta tattttaagc gcaggggagg gtgatacagg
     8641 tgctttaaat actttgctta ttcaacacct cacgccttat atgctacaaa cttatccttt
     8701 gttgagcatc cgtaatgcag ttaaagaagg taattttgat aaggatttgg aaatttttac
     8761 caaagcagct gtagatttcg ccaaacatga caggcgtttt attcatttat acaatgtgga
     8821 gggtaagtct ctatttttca aaacaatgta tgagtggatt gatgagctcc ccgcgactaa
     8881 gtttaagggg ttgcttgagt ctgcattaaa agattacgaa ggtaaattat ggtggtccac
     8941 ttcaaggaga tcggaggtgg aaggttattg tacaaaattc tcacaagcaa aaattgttgc
     9001 tatgaccttt ttaaacggga aagattcctc ctctctgaat gatgttttat ttgataaaat
     9061 catcgcggct atacaaaaag acataaacaa aaacaaagaa aaattaaaga taccaggatt
     9121 caggctaatt aattgctaca atgccaaaga acacagggct gattatttta aagaggtcaa
     9181 aaattacgca gaacctatat ctcataggca agaaactact ttgaattcaa atgttacgag
     9241 cttggtggtc taaattttct ctggcatgga gatttactcg tcttattgtg acaatacaat
     9301 aagctttagg ttattattct cttttttcag ttttgagtaa aaagtatgtt tcgtgatgta
     9361 ctgaagacac taccccaata ccttattccg aaacacggta tcactgcttt agcagggtat
     9421 tttgctgacg ttaagaaccc tcgtctcaag aatttcctaa ttcaaaactt tattcgtaag
     9481 tttgatgtcg acatgagtga ggcgcttatt gaagatccta agtcttatga ctgttttaat
     9541 gatttcttta ttcgtcattt aaagccggaa tgccgtcctc tttctcagtc agatgtgatt
     9601 tgtccagtag atggttgtat cagcgaaata ggcaagattg aacgaggaca gttattgcag
     9661 gctaaaggca aatattattc tgtacaagag cttttagcct gtgatgggca attggccgaa
     9721 caatttgtgc agggacaatt cgcaacgcta tacttatcgc cgaaagatta ccacagggta
     9781 catatgccaa tagatgcaga actcgtttca atgacttata ttcctggtgc tttgttttcc
     9841 gttcaaccag caactacacg tgttgttcct aaattgttcg cacgaaatga aaggcttgcc
     9901 atttttttta aaacgaaaat tggtccgatg gtgatggtga tggttggggc tactattgta
     9961 ggtgcaattg gaaccagttg gcatggtgat gtcaaaagat caaaagaatt agaacgcttt
    10021 gattatagtg agcagttttt ggacaagatt atatctcagg gaagtgaaat gggttatttt
    10081 aaattgggat ctacggtcgt tttattgttt gcaaatggag aaaaaataca atgggataaa
    10141 gaattattgg ctggtagcaa gattcagttg ggtaaaccaa tggcaattat tacttaatta
    10201 atggtaccga gaagaggact cgaacctcca cggggttgcc cccactagca cctgaagcta
    10261 gcgtgtctac caattccacc acctcggcat agggcgttaa gttacgcaaa ttatagtgtt
    10321 ctgtcaatac agaattgcgc gaaatcaaaa aagtataaat tacttgagat ttattgctgc
    10381 gaatagggga acccggcttt taatattctt tctcgtactc cctgccattc actcgtattg
    10441 ggtggtagct cgatagcgat acaaatggca tcggatgtat cagctaatct tagttgataa
    10501 tacaaatcat aggccgattt ttcaggatgc attcccagtg gatgaaagag attagtctcg
    10561 acaccagggg gttgtctatt cgctatgaca taaacagtcc ctttgcgttt ttgacaaaat
    10621 tcggaaaggg tttcatattg atcaaaataa tataaagttt tttgtggttg ataatggcta
    10681 tccaatttac cagggacacg aatattattt ttttgagtcg agcaacctat ggcaatcgtt
    10741 tcagcaatag ttttttcgtc tattattcca tggcgtaaaa tttgataaga aacaggattg
    10801 gtagcatcta caatggttga ttcaataccc acacgacagc gtcctccgtc caggatagtt
    10861 aactcggcat cattaaatga ttgtttaacg tgcaaagcgg ttgtcgggct aatttttcca
    10921 aaaggattgg cagagggtgc caccacagga atatttaatt tttgtaacaa tttttgtgtc
    10981 aagggatgat ccgggcatct tatcgctatg gtagattgac caccggtaat gagcggattg
    11041 atttgatctt ttttacaata gaataccagg gtcaatggtc caggccagaa cttgctagct
    11101 aattgatgcg catactcagg tacctcttcc acgagcttat gcagatccca attggaagca
    11161 atatgaacaa tcaaaggatg atttaaaggt ctgcctttca tttcaaacac agctctgatg
    11221 gcagcattat tgtttattgg tgctgccaat ccgtaaaccg tttcagtcgg tatagctact
    11281 ggttttcctt gctgtaaatc atgaattgcc cgattgagat tggtagtaat tatggacata
    11341 taaggggctc gataaaaaat agttatgttt cagggtcttt ctatctgttt ttaagctcaa
    11401 tttaataata agaataagct tctataaata aactggaggg aatcaggtag gatcaataac
    11461 ctgattttcc ctcaacccta tgctgtaaat tctattgcga taaactaata aattttagtc
    11521 tttctaaaaa attaaaactt aaattccagc aatttctaca tcggtttatt atagaacata
    11581 tggttcatat tgtggagtcc acatcgttct ttttatagaa tcatcgattt tattattgtt
    11641 cattggctct atatgaccct cttttatacc ctgtaaaatt accgctctcg ctatatgttg
    11701 actgacttct ctaatggaat ttaattctgg gagtaagcgt ccttctccgg ttctgattgc
    11761 aggtgctagc tcacttaatg caactgctgc tgccatcatc attaaatcag tcactctttt
    11821 ggcttgtcct gcaactactc caagaccaac tcccgggaaa atgtaagaat tattgcattg
    11881 agctatctca attttatgac cattaattac aactggctca aagggactgc ctgttgcaat
    11941 taaagcttta ccagctgtcc aatttaacag atcttgcggg attgcttctg cgcgagacgt
    12001 aggatttgat aaaggaaaaa ttatggggcg ttcacaatag cttagcatgg tcttgatcat
    12061 tgcttccttg aattggttag gttggccaga cacccccagg agtatagtcg gttgggcatt
    12121 gttaataacg tctgttaacg tgatttctcc ttttttttct aatttccaat tttggagact
    12181 ggtagaagaa cggacaaatc ctttttgaaa tggcaataga tctgtcatct catcatgtaa
    12241 aagtccatac ctgtcaacta aataaaaacg tgatctggct tcttcttcag aaagtccctg
    12301 gttcatcatg gcatgcacca gctgctcact gatcccacaa cccgctgatc ctgcgcctaa
    12361 taaagcaact cgatgttcct ttaaaggagt attagtaaca cgagttgccg ctagtatggc
    12421 ggctacagca acggaagcag ttccttgaat atcatcatta aaagtacaaa gttgattttt
    12481 atatcgctcc aataaaggat aggcatgttg ttgtgcaaaa tcttcgaatt gtaatagcac
    12541 atgaggcata tggcgtttta tactttgcac aaattgatca acaaaatcat catactcttt
    12601 gcctgaaatc cgagcgtgtc tccacccaat atattccgga tcgtccagac gctccttatt
    12661 attggttcct acatccagaa taataggtaa agtattagac ggatgtatac caccgcaact
    12721 ggtataaaga gacaatttgc ctataggaat tcccaatcca cctgcccctt gatctcccaa
    12781 ccccaagatc cgttcgccat ccgtgacaac aattactttg attgaacgag tcgacgcaat
    12841 gttttgtatg atgctgtcca gtttgtctcg ctctggataa cttagaaata ctcctcttgg
    12901 ttgccgataa atgtgggaaa acatttcaca ggcttggccg acaactggcg tgtatataat
    12961 gggcaaaata tcaaccagat tatcaattat aaaacgataa aagagtactt catttcggtc
    13021 ctgcaaggca cgtaagtaaa tgtgtttttc caaagggttt tctttggcac tataagcgtc
    13081 aaggcaacga attacttgtt gttcaagggt ttccactgtt gttggtatta aaccatgcaa
    13141 ggcaaattca tctctttcct ggaggctaaa tgcggtgcct ttatttagta tggggtctct
    13201 tagtaagtcc attccccttt ttgttacttt gattgccatt agaacactcc ttgtgaatat
    13261 ttgtagccaa gaaatttaaa tcaggatttc taaataactt caattcaact cattgatttt
    13321 aagatggaag ttatttacaa attttataaa tgctaattta ttgttgtatt ataattctca
    13381 catagtttct agtttagtcg atttttgcga ttttgtgcta atagcgtatt ttgcctttta
    13441 ttttatgccg gcataaaatg attaaaattg gactattagg ataattggtt ttgttactca
    13501 aatgcataga aaaaatagtt gatgggtatt tcgctgtaat tcccaggcac acaccggaat
    13561 tcgaaaatat caacggtgcg ttaataatcg cgcatagagg agctcataat aatgcaagag
    13621 ggataattga aaacactctg gaagcatttc agaacgcaaa agagaccgga tgttggggaa
    13681 ttgaactgga tgtacatctc actaaggaca aggtgttcgt tgttaatcat gatcctacct
    13741 taaatcgatt atggaacaag gacattgcta ttgctgatct gaattttagt gaattacggg
    13801 cattggagtg cagaatcccg tcgctctctg aaatagtgag tcagtatggg aattccatgc
    13861 atttatttat tgaattaaaa acagctattg aggaggaaga gatattagtg caaaccctgc
    13921 aacatttaac gccatgtaaa gattttcatt tattgacttt gcaggaatct acctttaaaa
    13981 aattgtgttc ttttcctagg caatctttat tgcttgttgc agtgcataat aacgttaaga
    14041 aattttgtga attaagcatc aaggaggggt atggaggcgt actgggtaat tatttattat
    14101 tgactaatag ggcattaaaa tatttacaag atgccaatca acattttggc gtgggatttg
    14161 ttgattccaa aaacagtctg tatagagaat taaatagagg ggttaaatgg ctctttacta
    14221 atgaggcaat aaacattaat cagtatttaa atgacttaaa aaatagcatg taatcatttt
    14281 gacaatactc ctgcttgttt ttgacaattc aataatcatc acatagtctt aaattaacag
    14341 ttccatttta tcatgtagca agggtaactt aaagatagca ggtttaaggt caggacattt
    14401 tcgtgagtga cggcaggggg tacaatgact ctaaaaatta cttttttagg ttccggatca
    14461 tcctttgcta ttgggccaga aaattatcaa tccaatgtgc ttttagagct agaaaacgat
    14521 acgttgctaa tcgatgcagg aacagatatt aaatactcat tgcgagatca aggcttgact
    14581 tataaagata taaaaaatgt ttatattagt cacttgcatt atgatcattg tggaggattg
    14641 gaatggttcg ctctaataac ctattttgat cctaatcact cacacaaacc ttgtctaatc
    14701 gcctctgata aaattattaa cgagttatgg gataaaagtt tatccggtgg tttaagcact
    14761 ttaccccatg agagagcaaa tcttagcgca ttttttgatg ttaaaccggt aaaacaatat
    14821 gaaggttttg tttggcaatc tataaggttt aaattggtac aaacagtcca ttattatagc
    14881 gaatatgagt taatgccaag ttttggtttg atatttaact ataataaatc acgcatcctt
    14941 tttacatcag acacacaaag ttgtccggta cagttaatat ccttttatga ggaatcagat
    15001 attatttttc atgattgtga aactacagaa tctaaaagcg gggtacatgc gcattattca
    15061 gagcttgttc aactcccccc tcatttaaag aaaaaaatgt ggctatatca ttataatcca
    15121 gggaaattac ctaatgccaa aaaggatggt ttcttaggtt ttgttgccaa ggggcaaagc
    15181 tttgtgtttt gatttaactt gtgcacatgc tcccggataa gccggggagc atgttgtctt
    15241 ctcactactg attggtgaac tggttaatag tacgagttat tcgatcgacc aattcatcaa
    15301 tttcatcttc actgataata agtggaggta ataatcttac tactgtttca gctgtaatat
    15361 tgaacaagac ttggttttcc aaacccagga cgcgcatgtc atttgcaggt ctgtccaact
    15421 ctattcctat catataacct ttaccacgta tagcttttac atggggatgc tctcccaggt
    15481 tgctaattaa tttatccatt aacaaagcgc tgtttttagt gacgcgctca catagcttat
    15541 ctcgttcaat gacctccaga acagtcaaag ccgttgcaca agctaaaggg ttgccaccaa
    15601 aagtcgagcc atgatttcca ggtttaaata aatctttagc tcgcttgctc attaaacaag
    15661 ccccaattgg catgccgttt ccaagtcctt ttgccgtagt aagaatgtca ggttgtatgt
    15721 cgtaatgcat acaagaataa agcttacctg ttctgccatt tcctgtttgt atttcatcca
    15781 aaattaacat ccagtcattt tgctcgcaaa ggttagccaa tgcacgcaga taaccttcct
    15841 ctgcgggata aattcccccc tcgccctgaa tcggttctac cataactgca accacatcct
    15901 ctctgttggc cgcaatagta tgaatagcat caagatcatt gaatggagca cgaataaacc
    15961 cagggaccaa gggttcaaaa ccagcttgaa ctttacgact gcctgaggca gttaaggtgg
    16021 ccatagtgcg accatgaaat gctttttcca ttacaataat agaaggcgtt tcaattccct
    16081 ttttatgacc aaacagacga gtaagtttga tggctgcttc attagcttct gccccagaat
    16141 tagcaaaaaa aacctgctcc attcgtgcca ttgaagtgag tttggcggct aataattctt
    16201 gttctttaat atgaaagaca ttagaagtat gtattagcct ggctgcctgc tcctgtatgg
    16261 tacgtgtgac gtcagggtgt gcatgaccta atccacaaac tgctatcccg cttaagccat
    16321 cgaggtattt tttcccctgc tcatcataca accacacgcc ttctccgtgc gtgaaggtta
    16381 caggcattgg gctgtaactt gtaattaaac tcatgatgtc tccataaaca taaaattatc
    16441 cggtaggatt gcaacaaatc cagcttgtta ttttatttca gattactgct cgcaaaatcc
    16501 caattaacta atgaccaaaa ggcttcaata taatccgggc gcctatttct ataatcgatg
    16561 taataagcgt gctcccatac gtcacaagtt aataaggcat ttaatccttc agtcattggt
    16621 gttccagcat tgcttgtgct gataattttt aaagcccctg actgatcctg gactagccat
    16681 gcccatccgg aaccgaatgt tgtagcagct gtttgtgaaa attgttcttt gaatgctgca
    16741 aaagaaccaa agcttttatt aatggcttcc gccagcctgc cttttggctc acctccacca
    16801 ttagggctca tgctgtgcca ataaaaggtg tgattccaaa cctgagcggc attgttaaaa
    16861 ataccacctt tagctttcat gatgatctct tccagagtca tggattcaaa ttctgttccg
    16921 ggaattaatt tatttaagtt ggtaacgtaa gtgttatggt gtttaccata atggtattcc
    16981 aaggtttctt tggaaacatg aggggccagt gcatccaatg cataaggtaa ttgcggcagg
    17041 gtaaaagtca tatcaatctc caggattaaa agtctttagt ttatcaggat aagtgtgata
    17101 ttcaatgtgc cattactgag cgattcaata tttttatagt cttggccatg gatacatttc
    17161 tgctggtata ataatctttt acgaaataga ggattttact tttcatcaag gtggggattt
    17221 ttggtgcatt tagtgctaaa ttggtatttt attgagcgtt gaggtggggt aaaatatcac
    17281 tataagaaaa agattgatag gattatcaca atagaatgat tagttgcgac cttgtttggt
    17341 tttcgacata attgggtttt ttattttata aaaggtgcat agtggatact ttagaaaaga
    17401 ttaaaaagca aattgctgaa aatgcgataa tgctctacat gaaaggcact cccaagatgc
    17461 ctcaatgcgg gttttctgct cgtgccgttc agtgtattga ggcttgtgga gtagactttg
    17521 cctatgtcga tatccttgct aatccggata ttcgtcaagt tttaccacaa ttttctgact
    17581 ggcctacctt tcctcaactt tatgtgaagg gtgagttaat tgggggatct gatatcatag
    17641 ccgaaatgtt tcagcaagga gagttagagc ctatgttgcg tgatgctgta gcggcataat
    17701 tttaataaca attgtgggat ttacttaccc accgctaatg gagattatag aatgagtgtt
    17761 ttagttgggc gtaaagcacc tgattttact gtggctgcag taatgggcaa tggggaaatt
    17821 gttgataaat tcaatttaca cgaacatttg aaaggcaaat acggattagt tttcttttat
    17881 cccctggatt ttacttttgt atgtccttcc gagttaattg ctcttgatca tcgtatcgaa
    17941 gagttcaaaa gacgtaatgt tgaggtggtt gctgtatcta ttgattcaca ttttacccat
    18001 aatgcctata ggaatactcc tgtgaaaaat ggcggaatag ggccagtgag atttactttg
    18061 gcagctgaca tgactcatag catttgccaa tcctatggtg tagaacaccc agttgctggc
    18121 gtggctttcc gaggcgcttt tgttatcgat acgaatggta tggttcgttc gcaaatcgtc
    18181 aatgacttgc cgataggaag aaatattgat gagattcttc gtatcataga tgcagtccaa
    18241 ttctttgaag aaaatggtga agtgtgtcct gctggctggc aaaaaggaca agctggtatg
    18301 aaagcgtctc ctaaaggtgt tgcagaatac ctgtctgagc attccgagtc cttgtaatat
    18361 aagacaagga aatagaatct ttttgttgtt gtgttttatt gagaagcata acacaagaca
    18421 atttttatca aactcctgga ttacagtttt aagtaatcca ggctatttat gtttttctga
    18481 tatcagatat ttagcattcg ccacctgttt atcataaggc aaaacaaatc agccgttttt
    18541 tcggcatcat aaatggcaga gtgcgcttcc tggctgtcaa aatgtaatcc agctgcttgc
    18601 accgctttgg ccaatacggt ttctccaaaa atcaaacccg ctaatgttgc tgtatcaaag
    18661 caagtgaagg catggaatgg tgattttatc ccggttcttt tgattgccgc tttgatgaac
    18721 aacaaatcaa accaggcatt atgccctact aaaacagctc tttggcaacg ggtttttttt
    18781 agcaagtcat ggatgggttt gaataatcgt tcaagggccg tttgttcatc taccgcaaaa
    18841 cgaagaggct ggtaaggatc tatttggttg aattccagtg atttttgatc cagttctgca
    18901 cccgggaaag gtaaaatatg ttcaaaataa ctactctcct tttttaacaa gccttgctca
    18961 tccatatcca ataagacaat acacatttcc aggagagcat ttttctgagg gtctattccc
    19021 gcagtttcta catcaacaac aacaggtaaa aagccacgaa agcgattttt aatggtgtta
    19081 cttagaatat tattgcgaga aaccattaat actccagttt atagtgtctc cagcagcaat
    19141 aggcacaaca tgagttgaac caaatgacat tgagttgggg atagtttgag gtgatttaat
    19201 gagttccaat tgctgttgat taatagggag ttggtaaaac tctgcgccaa atcgactaag
    19261 aaaatcatta agttttttta attggttaag ttcatcaaag atctgcgtgt agagcgctac
    19321 cgcatatgga gctgaataaa taccggcgca tccacatgca ttttccttgg cattgatggc
    19381 atgaggggca ctgtcagtgc cggcaaaaaa ttttggattt ccgctcgttg ctgcaacttg
    19441 tagggccttt tggtcttttt cgtgttttaa aataggcaaa caataataat gaggtctaat
    19501 gccccctgcg agtagcttat tgcgattata aagtaaatgg tgaggagtta ttgttgctgt
    19561 aaggttagca ggtccttctg aaacataatc tacggcagct ttagttgata tatgctccag
    19621 aacgattctt aatttgggga aatgtttggc aatgggtttc aggtattcat caataaatag
    19681 tgcttcgcgt tcgaaaatat cactatgagt gacttcgcca tgaatttgca agactaaatt
    19741 actcgtttgc aatgtttcaa aaagggggta gagtgctttc aaagatttag caccctcttc
    19801 agaatttgtc gttgctccag ctggataaag ttttgctcca actatataag aaagagaggc
    19861 ggcttgctgt agttcctctg gtttaacgga ctcatttaaa taaaaggtca tataaggaat
    19921 aaaggagttt tgtttgggaa tggccgaaat gattctgttt ctataactct caattgcggg
    19981 taaattagtt agtgctggtt tgagattggg catcactaac gctctggcaa aatgttgggc
    20041 agacgcctgg acggtatgag ttagaaactc tccatcccgg agatggagat gccaatcgtc
    20101 agggcgatta attatcagag tttgcataat aaaggttcct gcattattgc cgataaaaga
    20161 gaataacatg aattaggcaa ttgggatgag caaaaaatga tttcagatca ttttatatgc
    20221 aagaaagcaa aaattattgc aatgagttct ttgttcatta ctactgtcat ctttgctgct
    20281 ccctctatcc aatatgcaag ccctatggga gaagaaaatt ggcgtatgac aggcaatcct
    20341 cttcgatgtg gcttgtcatt gacgattcca aattatggta ttggctactt tgaacaatac
    20401 gctacccaat cccctcactt cattctgcgt aaatgggatc aagttcaacg ggccttacct
    20461 gctcaagttg tagcaagccc cccagtgtgg aagccattat tgaaagggtc ctatctggta
    20521 acccgcactt atataaaacc cggggagtat ggactatttt taaatcgaca gcccgcttta
    20581 aaattattga cgtttttatc gcaaggatat caggcaaatt ttagctattt atcagaggaa
    20641 ggatttcgca ctacggttat actctcacct gttcggtttc aaaaagtgta ctcgcagtat
    20701 caacgttgtc ttggtggtct gttgcccttc aactatgagg atgtaaaaga aagcgtgttt
    20761 cattttgggg tggatagtaa gttattgtct gatgctgata aagtgcaatt acgacggatt
    20821 gcccaatacg tggatgctga tgtacaaatt gatgtcatta agattattgg tcatgctgac
    20881 gatagtggcc gtaaaggtta caataatgcc atttcacaat atcgagcaga gtctgtgaaa
    20941 aattatttga ttaggcttgg agtacctcaa cgaaaactat caataacatg ggtaggagct
    21001 ctgcaaccga tagccaggaa tgatactgac gaggggagag ctgctaacag acgtgttgtg
    21061 gtgacattga taaaaaaata aatcttgtga ttaaaaaagt atctttttat gatggcattt
    21121 catcctcgaa aatccccttc ttggtcaaca aatttcattt gaaagagaca tggcaataat
    21181 gttaa
//
LOCUS       DASLOE010000039        20614 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.039, whole genome shotgun sequence.
ACCESSION   DASLOE010000039 DASLOE010000000
VERSION     DASLOE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20614
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.039"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(270..647)
                     /gene="pieF"
                     /locus_tag="U6309_001160"
     CDS             complement(270..647)
                     /gene="pieF"
                     /locus_tag="U6309_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PieF"
                     /protein_id="HEN5504611.1"
                     /translation="MKRLIICNGNKLTVCTQAISSGDIVEKYTPIFSLTKESDNELTL
                     ELSGIARGYYIIPSELNSSQEKAAHLITLLTRAEESQVTDMHKILNSFVSGKITSGSM
                     FNFENDGSFKREPEEAYNLINKI"
     gene            1492..1734
                     /locus_tag="U6309_001161"
     CDS             1492..1734
                     /locus_tag="U6309_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504612.1"
                     /translation="MTNKILTERLNNELDELGVPCLMTERVQVCSKLFNLPKFKMEAL
                     LHGVIAIDANSIQKIADELEVNKDWLLGGIKKKTKH"
     gene            2206..3099
                     /locus_tag="U6309_001162"
     CDS             2206..3099
                     /locus_tag="U6309_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504613.1"
                     /translation="MFSLKKTTAAVLALGSSALFAGTMGPVCTPGNVTVPCERTAWDV
                     GITALYLQPVYDADWGYNGFTDVGGWRNWHDVDLEWDWGFKLEGSYHFNTGNDINVNW
                     YHLDANTDHWAFVDQLHAYDITWDAVNAELGQFVDFSANKKMRFHGGVQYAYIKTDVN
                     RHLNGLFLDNFNSKFNGFGPRTGLDMNYVFGNGFGVYAKSAVAILVGTSKFLDNCTVC
                     GYSYGSKNAIVPEVEMKLGADYTYAMAQGDLTLDVGYMWFNYFNALHNTAAVNVGLGT
                     SWETDFSASGPYIGLKYVGNV"
     gene            3734..4352
                     /locus_tag="U6309_001163"
                     /pseudo
     CDS             3734..4352
                     /locus_tag="U6309_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947691.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrB"
     gene            4361..5221
                     /locus_tag="U6309_001164"
     CDS             4361..5221
                     /locus_tag="U6309_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrB"
                     /protein_id="HEN5504614.1"
                     /translation="MSLQLHKINSKARFETTIEERDCQLPIPLPDTLKTVTKINSGVW
                     HTVIAGSDQHNRPQLATFGKNCDGQLGTGDWENRDTPTLVKLPPEMHTLHVVQTGFFH
                     TIIAGLDKNGWPIVASCGYNSHGQLGSGDKDNKNTLIPIKLPENMKSVEMVAAGVKHT
                     IVVGKDINNKPVVATCGHNGYGQLGTGDEENRLCLTPIEIPEDITTVDYIEAGAYHTV
                     ICGRNMKNQPILALCGCNSDGELGLAPQKIAKKSSLMTNPALFFPPRQDRNAINNVMK
                     ISKFSPCNLL"
     gene            5409..5993
                     /locus_tag="U6309_001165"
     CDS             5409..5993
                     /locus_tag="U6309_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEN5504615.1"
                     /translation="MPTSKKILMIVGDFNEDLEVYFPYQAMLMNNYLIDTVSPGKNKG
                     DFIQTAVHDLDKPHLQSYTEKLGHLFPITADFSKITLKDYDALIIPGGRAAEYQRLNK
                     DILAITRHFHETNKPIACICHGIQILAEAGILENKKCTTVVFCEPDVRKAGGHFIDKG
                     MDGVVVDGKLVTGATWLGNAPWMREFLHILQNEE"
     gene            complement(6063..7997)
                     /locus_tag="U6309_001166"
     CDS             complement(6063..7997)
                     /locus_tag="U6309_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein"
                     /protein_id="HEN5504616.1"
                     /translation="MYKMYSYPGWRIDMKTENLPQTGQEAQIDKKIHFIWVGHIMPQK
                     NIQVVSEWAEKNPGYETIIWVDKKIAPAKELDLFIQDMKSKGITVKDINEEGVCRDSI
                     RHELDQESPNYGMASDMLRLNILAAEGGIYLDSDILCSTPFPDEIHAPFGFLLSPWSQ
                     GANNALCNDIILCSKGNQIIQQLADAIEQSYIARDSFEFTHEYASMKETKGERVAKTL
                     GVTGPGFLFHQLKKMGVLNDKSELSAVHWELQDQRYLINGSVEAPDYFYVPQNNKNDA
                     SWVPSIKRPGIENMSFQERLENAVQLIAFNIQKTGLFNLDHYANELKVKQNNWCIAAE
                     TPSELKPDSYLLIRPRDKTGEWALYYVDEDKKLNPVTLPVIKGAIKLSELSDPLRKFH
                     IVLSQVSDPVNPTTHELKQIGRALMDLKPKQNEWQCKNKWPGVEEIAQELWQRVTSNE
                     TLRAQIKQCSTQFESLKPRVAELGLTRVSEAETEVEEHESTVKEQEIISQKTVVEECT
                     QEKNSAQLAPENPSEEKIKTAHDLIDEIIQDVIQLDGKLGLLGGNTRQLENGKVINIP
                     NGAAMIFDDYKKYKQGELTAEGALESMIKIAKLSNKLNRHTFFNQRQPETGQFYKKVA
                     AIDLQTTIAAEYDNNHGLRM"
     gene            8233..9600
                     /locus_tag="U6309_001167"
     CDS             8233..9600
                     /locus_tag="U6309_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5624 domain-containing protein"
                     /protein_id="HEN5504617.1"
                     /translation="MKTLMTCIFSLIILFSQPFKPIVFAHTNNTNNQVLSASANNSVG
                     SNVPNNIYVTPQAFWNLYFDFTGDETPGYPKGKINISQTLFQSEMKKNSSLAQQNEGQ
                     LILFINSTLYIYNSDRQLELKQLMRTAPNSGFSEMTAISHIGPALMYLAKIKENGDAS
                     WKSQMENLLKDIQAVKVINAQTPNNWLEQVNAPAWKPHLTTIHNMIDYACSMAGNYMS
                     DVLNEKLSFDMASLQNDFLNGNKTYPIPYNNVMIGTFMLTALQSMDQLHSKISQLKID
                     WPHAKVIIRFVAGSNVSAGVTKGSNWLVPFVQALSNNTLAADRIYITPYAAVKPSLGA
                     QELTQTDYNYYNNTVWGARHNRTIIANEVFTNITSIFLPDRPAIPGDYTYSKQPKIED
                     FLMRLKFSLAEPTEMLSNTVGFWMAGELAEKKWNYNKISIPGITTGFPQGISTYPNNN
                     PVIQR"
     gene            9597..10745
                     /locus_tag="U6309_001168"
     CDS             9597..10745
                     /locus_tag="U6309_001168"
                     /inference="COORDINATES: protein motif:HMM:NF015681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504618.1"
                     /translation="MINKLLVSTILLLIFPISGQSKTVLAPYQYLKTSGARDITPFTL
                     DGAQYLAIPQLSKDEPDIPANINGGNAKVDVLIFKWINGKFHLYQKIPGHGNEGTAFF
                     RIKDLAYLATSSIYSKPKTANNKHSYTKLYRWNGRRFYPIQQFFTYASKGILAFSIGE
                     RHFLAFADGVVLPNNKITTNTDSIIYEWNGKKFIPFQSFPTKWAYGWDFFTLNGSNYL
                     ALTDHAQASRIYRWDGKKFILFQSFSKTGGRAFSHFTIDGKFYLAYANLLNPSIIYQW
                     AGNQFVEYQPLEGLGGRNFVYFSSHGKHYLLRINFITGSRIKPKTKLSSQLYQWINGK
                     FKVVQNITTSGGVDAAVFSVNNQRYLGVANSLNERSRFQTDSIIYKIE"
     gene            10847..12511
                     /locus_tag="U6309_001169"
     CDS             10847..12511
                     /locus_tag="U6309_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504619.1"
                     /translation="MKDFVIVGAGPIGLYWAARLAQIKESSGLPINIVVIDPRAGNYN
                     RERIVSNEAIKSISSRFSISMPSGKGIPSEAMYIRDFEKSLYEYCESKGVKFKKGTFS
                     GLSYNLVSYKDTKENIRTVKCDMVVDCSGSNRMVLKETNRLLGKEVFEIKTLGKNPHT
                     NHFSCYMSLSTEEAKKLLIPDEIDPLLQAKQLCKLRQKYDWPNFSFPVVDIRSAKNEE
                     GGVNYFIYYEVPDNLKEKDKSVQIEFLKDLLKLKYGFEDIDFTIHSFGHFPVCPKYVD
                     KPFYLSEDLPVVLAGGDCQIEPDYRKGIGIESGIERANFLFDTVHGTSKGLGFLFDNY
                     YQQVARYVGYHGRLIEQFYLQRVENINGSSLEQAKKILCSACESVKEVEDVAAIAGEL
                     KLLGNELFKKPNYESALECYLNAIHLYQSFEKALPLTMDFVTLHSNACQTCLKLKEYG
                     QCINLANEGIKAYAEIKAEDKDMLFKLLFRKASALVELGNGLDAKTQRKELDELLNDL
                     KETYELMQANSGVNNATFVKQIQSKIVNIEKKLPPPQEEVNKIEFI"
     gene            12695..13894
                     /locus_tag="U6309_001170"
     CDS             12695..13894
                     /locus_tag="U6309_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HEN5504620.1"
                     /translation="MLNKGYIKTTPYGLGFSELFFEDNLKKYFSDPELEVLSAEYSPS
                     ASDKMAWSVKHPKQGDHPIGVQRYKISLKKNGKIQTRHVIAKSKISDKQYLQIISNAF
                     AKCGIQTEIPISDYLSQLEFSNLNRKEITIYYMQDSFEAFRHAMPLCYGSYLDEQEEI
                     CVLILECLDEEWLSLDPYNTSKWDQTAIEALIDAIASMHAVWYRKEKAIAEIKGFKNP
                     LNIDQMQRFMPYWNALAKAVKTSNLPFLTKKDYLFHTHLIETIPQWHIKIDAMAKTLV
                     QNGCVPKNIALCNQNGVKKVLVYDWEIATIHIPQRDIVEFLSYVLPENFDSKVLGNYI
                     ERHRIKLGETTKQAIDATEWRLGFQYALYDYLIQRVFPQIPFEKLEARNIAKVYNNAR
                     RMMALLD"
     gene            13914..14651
                     /locus_tag="U6309_001171"
     CDS             13914..14651
                     /locus_tag="U6309_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HEN5504621.1"
                     /translation="MTHIRVILPGYNTKVLNKEMLKPFESPGLELSVDYADIAVDAVR
                     REFDIALMAPYLIKKVIKAEQEKVHAIVIDMMADVAMRALREAVTIPVVSLAETATHV
                     AAMMGNNFAIINTTDELTPIQESLVRSYGLYDRLACIQGIQLDPHDDYRSEKTIHGLV
                     EQCADAVIKKHADTLIFGSGRLIGYREVIQKGLEEKGLHITIIEPLPTGIYFAKFLVD
                     SKISQSKYVYRSPDASTLKTYSKILGN"
     gene            complement(14691..16004)
                     /locus_tag="U6309_001172"
     CDS             complement(14691..16004)
                     /locus_tag="U6309_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN5504622.1"
                     /translation="MNSILHLILGDIITPTQTGQSLLIKKGYVLIDGDSIIEVGEQAH
                     LNQEISCTVSNYPDHLILPGLIDTHSHLSQYAISGSGDLPLMDWLNALVFPAEAAFAN
                     DLKRCQRHAELFMHACLGSGTTTINTMVTSSRKATEVVCDVAARMGIRAFIGLVLMDR
                     NAPGSLLVDRDQAFKDLTFLKDNYHGKNNIHITVSPRFAVTCSAAMLKQAGEFARANK
                     LILHTHLDKDEGFDELIQSLFSTAHDYFDVFESTQCIADKTVFAHGTLLSLHEMKRMG
                     DYAGQVGISHCPSSNFSFAMGMAPVSFFKDLGIEVGLGSDSGGGDSLSLFDEMRSASF
                     TNKALWRLDKKTALIDAKSWLYHATLGGAKLLGLEKQIGSIEAGKKADLIILDDRNNY
                     PLAELSSLSQELALDELQYRLARVIARSCENKLVAVYIDGKKVYQ"
     gene            complement(16186..19035)
                     /locus_tag="U6309_001173"
     CDS             complement(16186..19035)
                     /locus_tag="U6309_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEN5504623.1"
                     /translation="MQKFSLHLSEVIGMGKTTTPEINQLNKYIIDKKSIKLANDLWVR
                     SVPLKDTPAEKYIVGTRKIPAVVAEKLQIRSLKGPIGIPEFDKNKPYKDYVVVPVYNL
                     DEELVGLQIIQIDPAGNKATNPENSDFFCKKYLGPTKPLREGKAAVINKTDNVNVVFI
                     AEGVETAASVASLHEVRNNYSVLASMGVSELLATLAYVKTHYPPGATVVLLKDHDDPV
                     ENPFANSEFDKAKKAFVDAGYRVIVKEPPTVNEDWNDVLAKGGVSDLERQINLNDPIL
                     CLPDIPARNSEVTPHFKSLYVDLLKNEKLAEEQAICALLDKVIGQLIDKLKKTQSSED
                     FHKIIKEIDEVVRTTEGVRTLLSETKNIPYQLRGLRRALKELKSLEDRRRDSLRDNEA
                     IDFSTDPQTNSNGDLFKAYTYVMRHCLKHLQSLKPTDIFDLKKHSEILGFYEKRLNAI
                     DNEIKRIPQLIAEFSGDDEIVGEIVIKQLKNKLKSLKLERRLYAQEFHEMKQQLRLLR
                     TAHPKGNTPYANYYEQFVENANSIMMKGKTQTKAIREELTGVWANKKQDLQEYYKKRI
                     EAARQNFASERKKTIPPLIEYLEGLRAIVKKRSEEIDKKALLPESRNYQVRYLQAMEE
                     LGQSEGVDKILQDWLNSLKDFKTASPLIYYSPKEGEIVDGVELIDYDSEGEETLDELC
                     KEIFVDKERLLETPMNERDNGKEKREEKEGDSDPRAGVSTPVLSGPSHLAEGLMQSIQ
                     PSSLKASDPQLYLTIKDFSERLAVSLYKSFEVISPTSGERQEFDGLAFRKKRPTIIER
                     KTNDGTGPGLFQRNFCQQKIVSKHDFVLKNVIAELKTQSHPEEYVDIDVPDYLSWYSK
                     NFTPEMQERLVNAAKSTVIKALQNLTFEFNVNRPKAYSATDYRGLFFNRGLIPGGVSL
                     RFSSLGKGNEEVAHQHMDKISEERTAKRESEIHFI"
     gene            complement(19213..19680)
                     /gene="sixA"
                     /locus_tag="U6309_001174"
     CDS             complement(19213..19680)
                     /gene="sixA"
                     /locus_tag="U6309_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEN5504624.1"
                     /translation="MKIYLVQHGDSLDKDIDPERPLSDQGQKDIQLLSNFLSPLKIEV
                     SCIYHSGKLRAQQTAESLSGHILSKNGVESRAGLEPLDDVIPLAKEINQLKQNMMFVG
                     HLPFMNKLVSQLVAKEEHRILVSFKPGSLVCLDKADGIDWCIAWMICPEFLRA"
     gene            19755..20300
                     /locus_tag="U6309_001175"
     CDS             19755..20300
                     /locus_tag="U6309_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN5504625.1"
                     /translation="MKPKISVFIASSLDGYIARNDGSIDWLIEANSLASPGEDCGYRS
                     FIATIDTIIMGRYSYEKVITFDEWPYGSIPVIVMSSKPIQIPEHLQHIITVSNQSPPD
                     LVSELSLHGIQHVYLDGGITIQGFLQNNLVNEMIITLIPVLIGSGRPLFGSLKNDIKL
                     KHRLTTSYPGGFVQIKYQIGE"
     gene            20399..>20614
                     /locus_tag="U6309_001176"
     CDS             20399..>20614
                     /locus_tag="U6309_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEN5504626.1"
                     /translation="MDIRYLIYAWGSSILFFIYLYLSYLVIIQRRKYHVLYLTNDDKA
                     FIARFRAHANFAEYVPLTLILMGMSIIL"
BASE COUNT         6502 a         4048 c         3691 g         6373 t
ORIGIN      
        1 gcccaccatg ggtcttggca attgttgtat acaatgcttt cataatttta tattctttaa
       61 aatggtaaga aattaaaatt atttagtatt ttgctgtttg tcaaacagaa aggaagaaat
      121 ttcatgccgc actttaattc gtgcctggaa agaaaacagc cttgataagc aaggaaaaga
      181 cgatacaaac ttaaattagt tacaaattga gaaaatcagt ctcaataatc agaaaacata
      241 taaccatccc tggcttatta gaaagcttat caaattttgt ttattaagtt ataagcttct
      301 tcaggttctc tcttaaatga accatcgttt tcaaaattaa acatgctgcc tgacgtgatt
      361 tttcccgaaa caaacgagtt aagtatttta tgcatatccg taacttgaga ttcttctgcg
      421 cgagtaagaa gtgtaataag atgtgctgct ttttcctgag aagaatttaa ctccgaagga
      481 attatataat atcctctggc aattccagac aattctaatg tgagctcatt gtcggattct
      541 tttgtaagac tgaaaatggg tgtatatttc tcaactatat caccagaaga aatcgcttgt
      601 gtacacactg ttaatttatt gccattacag ataatcagtc ttttcattag ttagctcgca
      661 ttttggaaaa ataaagggct ttgattatat aaaaataaaa ccaaacagta aatatttgat
      721 cttttgtgat tacattaaaa ctattagagc ttacaaaaat caataagatc aaaagatggc
      781 ataatgaatt gaattaagat tgatagaata gaccttttgc ataaactaat tattttattt
      841 tagaacaaac cacagatatt ttaaggagtg gagtttatat gcagtaaaac aaaaccaaca
      901 tgagcacgtt gcttacatcc tgaccttcaa aaaatttctt ggtaaaccaa gggctgtcta
      961 tgaatataaa aaggaggaac ccagtaaagc caagctaatt tgcagtggaa ccatgatatt
     1021 tttattcaaa gttaatcaat cctgtccttt taaatcatac aatttcaata catctcatca
     1081 gtaaataatg tcacttagaa aaataatcta aaaatactga caacccagat tggagctctg
     1141 ctttccgcat tcaaagaaag tgctttaaac taaaaatgcc ctaaaataat ttcagttatt
     1201 tctcaaaaat ggcgctgcag atatacttca tgaatacagc attcgctgat tctcagcttg
     1261 tttgaaaata gataacaacc gaacgcagtg tttggcctaa aagataatag agtctgtttt
     1321 atctactcaa acggacatcc ctttcaaatc accaaacaat tcgccattgg atttttcata
     1381 ttttcagaga ctgggatcag gctttcaatt aattatctga caaaattaat ttgttaatca
     1441 atccttaagc tttagtgttt tacactcata taaaacgaga aggagaatac catgactaac
     1501 aaaatattaa ctgaacgctt gaataatgag ctggatgaac ttggtgttcc atgtctaatg
     1561 accgagcgtg tgcaagtgtg ctccaaacta tttaacttgc ctaaattcaa aatggaagcc
     1621 ttattgcatg gtgtcattgc tattgatgcc aactcgatac aaaaaattgc cgatgaactt
     1681 gaggtgaata aggattggct actgggcggc attaagaaaa aaacaaagca ctagaggttg
     1741 ttgacatttt ctcagacagc tgacctactg cggctttgtc cgcagtatcc tacagacttg
     1801 actcaggatc tctgtattta taaagccact aatccatatt tatgttgcca atttttaaaa
     1861 tagcattttc ctgaaatgac aggatttaat gattttttcg aaggatatct tgaatctctc
     1921 cactattctg gtttttgtct accattgtat actcactggc atcatgtgct tccccacccg
     1981 ttacttcaaa atccaaggga aaagcaggct aattgttgaa gtataaatca acaacagtac
     2041 atattgatca aatcttatat ttaatgctta aatcaatgag ttagataatc ttaatataat
     2101 aaaattacct ctattattag atgaagaatc aaattgattc tgttagaatt ggtttatcct
     2161 agatgttcaa aggaataaca ataataaatc agtggagaac aggatatgtt tagtttgaaa
     2221 aaaacaactg cggctgtact tgctctagga agcagtgctt tgtttgccgg tacgatggga
     2281 ccagtatgta ctccaggcaa cgtaactgtt ccttgcgaga gaacagcatg ggatgttggt
     2341 attaccgcgc tctatctgca gccagtttat gatgctgatt ggggctataa tggttttaca
     2401 gatgttggtg gctggagaaa ttggcatgac gttgatttag agtgggattg gggctttaaa
     2461 ctagaaggtt cttatcactt caacacaggc aatgacatca acgttaattg gtaccattta
     2521 gatgccaaca ccgatcattg ggcgtttgtt gatcaattgc acgcctacga tattacctgg
     2581 gatgctgtga atgctgaact gggacagttt gttgatttta gcgcgaacaa aaaaatgcgc
     2641 ttccatggcg gtgttcagta tgcctacatc aaaacagacg ttaatcgtca cttaaatggt
     2701 ttatttctcg ataacttcaa ttccaaattc aatggatttg gtcctcgtac cggccttgat
     2761 atgaattatg tattcggtaa cgggttcgga gtttatgcga aatcagctgt tgctatcttg
     2821 gttggtacga gtaagttcct ggataactgc actgtctgtg gatattctta tggttccaaa
     2881 aatgcaattg tgccagaagt tgaaatgaag ctgggagcag attataccta tgcaatggcc
     2941 caaggtgatt tgactctgga cgttggttac atgtggttta attacttcaa tgcactgcat
     3001 aatactgctg ccgtgaatgt tggattaggg acttcttggg aaaccgactt ctctgcatct
     3061 ggcccctaca taggcttaaa atacgttggc aatgtctaaa taatccaaaa ttatgctgct
     3121 aatctagtaa tgatcaagca gccgtatgca aaaaacccgt attgacatag gctaatgcgg
     3181 gtttttttta tttctctctg tttaattaaa ttagttatcc tctccaaact tacttgtaat
     3241 agtagcgatc caaaatagaa tttggtaaaa gcatttcaat ttatatcaag caaacactat
     3301 tgcaaaaaac aaatgagtaa tcttgatgaa ctatttaacc agctaagcta actgattcat
     3361 gctcacaagc aaaaatactc actggttgaa cgaaactcca tgattgagat tctaaaaaag
     3421 ttaaacacca gttggatgtc gagagatatt atgaaaattc atagtaacat cctaaattgt
     3481 ctaaaaatag aaaaacacaa ttgggcagca gattctgatg aagtccaaga gaatagaaaa
     3541 aatcatcact cctggcctta ttgctttatt ttatgatagt tttttagtca tattgagatt
     3601 atttaatgta caaataagga ttgggtaaca tgaccaatga aaaatcacat ccgtgatctg
     3661 ctgtgcagac tataggaaac gcaataagcc gctgattctg cctgatgcat aattctcttc
     3721 cattccaaag atcgtgggtt ataatgaaac catctgtcac caaccgttta taaaaataaa
     3781 catgcctgat gttaacggca aacctgatgc aaaccagatt ccagatgacg tctggcttga
     3841 gattcttatc aattatcttc aaccacagga tgctgtgaac ctgtctgaag tcaacaatcg
     3901 cctgaaccga ctttttgcaa ctcaacatac tcaatctgag caattgaacc gaaaaaaaac
     3961 attaggaatt ctgaaggtat ttacaggata tagttccacc ttcctgttta tacgcttcaa
     4021 tggcaaacaa agattattta aaacaagaaa ctggagtgta gtagatagcg ataacataac
     4081 cccactatta atgcccatct ctctaccaga gaacatctct tttattgaat ccattcaatc
     4141 aacatacaaa gacactattg ttgttggccg tgataatcgc ggtcaaatta tagtggcaac
     4201 aaataaaatc cataaggatt attcaaggca agaaaccagc cacaatcagc tgattcccct
     4261 tccattaagc aacaataaca tttctctggt atctgccggg aatgaagaga gctatatttt
     4321 taaccctatt tttctcttat cccgcgatgt agcagggcaa ttgagccttc aattacataa
     4381 aataaatagc aaggctcgat ttgaaaccac aattgaggaa agagactgcc agttgcccat
     4441 tccattgcct gacacgctta agacagtaac aaaaataaat tcaggagtat ggcataccgt
     4501 cattgcaggc tctgatcaac ataaccgacc acaacttgcc acctttggaa aaaactgcga
     4561 tggccaatta ggtactgggg attgggaaaa ccgagatacg cccaccctgg taaagcttcc
     4621 ccctgaaatg catacgcttc atgtagtcca aaccggattt tttcacacca ttatcgctgg
     4681 gcttgataaa aatggatggc ccatcgttgc aagctgcggt tataactccc atggccaatt
     4741 gggaagtggg gataaagata ataaaaacac attgattccc atcaaacttc cagaaaatat
     4801 gaaatcagta gaaatggtcg cagctggagt gaaacatacc atcgttgtgg gaaaagacat
     4861 aaataacaag cctgtggttg caacctgcgg gcataacggt tatgggcagc tgggtacagg
     4921 cgatgaagag aaccggctct gcttaactcc catcgagatt cccgaggaca ttacaactgt
     4981 cgattatatt gaagcaggcg cctatcatac tgttatttgc gggcggaaca tgaaaaatca
     5041 gccgattctt gctttatgtg ggtgtaattc agacggtgaa ttggggcttg ctccacaaaa
     5101 aatcgccaaa aaatcatccc tgatgaccaa cccggccctg ttctttccac cgagacaaga
     5161 caggaacgca ataaacaatg tgatgaaaat tagtaaattc tcgccatgta atttgctatg
     5221 aaatttaatt tgtttccata gagaaaaatg aagttctaaa atcttattat tttccggaag
     5281 aaaagaacca atcccgctac caaactactc tcttacttat atctgctctg atagacaagc
     5341 gtttctggac aagtttaatt aatagctgcc atacttaatg aattcaattt gtaaaaatgg
     5401 aatgcatcat gccgacttca aaaaaaattc ttatgattgt aggagacttt aacgaagatt
     5461 tggaagttta ctttccctat caagcaatgc tcatgaacaa ttacctcatc gatactgttt
     5521 cacccggcaa aaataaagga gattttattc agaccgctgt acatgatttg gataaaccgc
     5581 atcttcaatc ttacaccgaa aaattaggac acctattccc cattactgct gatttctcca
     5641 aaatcacttt aaaggattat gatgctttaa tcatccctgg tggaagagcc gccgaatatc
     5701 aacgtttgaa caaagatatt ttagctataa ccagacattt tcatgagaca aataaaccca
     5761 ttgcctgcat ttgtcatggc atacaaattc tcgctgaggc cgggatactt gagaataaaa
     5821 aatgcacaac tgtcgttttt tgtgaaccag atgttcgaaa agcaggagga cattttatcg
     5881 acaaaggaat ggatggtgtt gtggttgatg gtaaattggt tactggagca acatggctag
     5941 gaaatgcccc ctggatgaga gaatttcttc atatcttgca aaatgaggaa taatcatttt
     6001 gcagagttat tgaccagtgg caatcagatt aaatctaaac caattcaatg tttatataaa
     6061 aactacattc ttaacccatg attgttatcg tattccgctg caatagttgt ttgtaaatca
     6121 attgcagcca cttttttata gaattgcccg gtttctggtt gcctttgatt aaaaaaagta
     6181 tgccgattaa gtttattaga cagttttgcg atttttatca tagattctaa cgcaccttca
     6241 gctgttaact ctccttgctt atatttttta taatcatcaa atatcatagc agcgccatta
     6301 ggtatattaa taaccttgcc attttccagc tggcgtgtat tcccacccaa tagacctaat
     6361 tttccatcca gctgaatcac atcctgaata atttcatcaa ttaaatcatg agcagtttta
     6421 atcttctcct cagatgggtt ttctggcgct aattgggcag aattcttttc ttgagtgcat
     6481 tcctctacaa cagttttctg cgagataatt tcttgttctt taacagtaga ctcatgctct
     6541 tcaacttctg tttccgcttc agagactctt gttaaaccta actcagctac ccttggtttt
     6601 aatgattcaa attgagtgga acattgttta atttgtgctc ttaacgtttc atttgaagta
     6661 actctttgcc aaagctcctg tgcaatctcc tcaacaccag gccatttgtt tttgcattgc
     6721 cattcatttt gcttaggctt aaggtccatt aaggctcttc caatttgctt taattcatga
     6781 gttgttggat ttacagggtc agaaacctga cttaatacta tatgaaattt tctgagagga
     6841 tctgagagtt ctgaaagttt aatagctcct ttaataactg gtaatgtcac aggatttaat
     6901 tttttatcct catcaacata atatagagcc cattccccag ttttatccct gggtctaatc
     6961 aacaaatagg aatccggttt taactcagag ggtgtttctg cagcaataca ccagttgttt
     7021 tgtttcactt ttagttcatt ggcataatga tccaaattaa ataaaccagt cttttgaata
     7081 ttaaaggcta tcagttggac tgcattttca agtctttctt gaaaactcat gttttctatc
     7141 cccggccttt tgatactggg aacccaactg gcgtcatttt tattgttctg tggtacataa
     7201 aaataatctg gcgcctccac actcccattg attaaataac gttgatcttg taattcccaa
     7261 tgaaccgcac taagttcgct tttgtcattc aaaactccca ttttttttaa ctgatgaaac
     7321 aaaaatcctg ggccagttac tcctaatgtt ttagcaactc tctccccttt tgtttccttc
     7381 attgatgcat attcatgagt gaactcaaaa gaatctctcg caatataaga ttgctcgatc
     7441 gcatctgcta attgctggat tatttgattt cctttgctac ataaaatgat gtcattgcaa
     7501 agagcattat ttgctccctg agaccaggga gacaataaaa aaccaaaagg cgcgtgaatt
     7561 tcatcaggga aaggcgtgga acataagata tctgaatcca gataaatccc tccctctgca
     7621 gccaagatgt ttaatcgcaa catatcactc gccatcccat aattaggaga ttcctgatcg
     7681 agttcgtgac gtatactgtc ccggcacaca ccttcttcat taatatcttt taccgtgatg
     7741 cctttggatt tcatgtcttg aataaaaaga tcgagttctt tggcaggcgc tattttttta
     7801 tctacccaga taatcgtttc atagccaggg ttcttttcag cccattcgga gacaacctgg
     7861 atgttttttt gaggcataat atgaccaacc catataaaat gaattttttt gtcaatttga
     7921 gcctcttgac cagtctgtgg taaattttct gtcttcatat caattctcca acctggatat
     7981 gaatacattt tatacagatt gatattaagc ccggatgagc atcataagaa taaaactcat
     8041 agtacatgcc tgttaagaat agccagaacc taacgagagc gattaagaaa taaagaataa
     8101 ttacaagccg atttcccagg tcctaaccca tgagtgtcga tggatttccg taacgattat
     8161 caatataatt ttccggtaaa tttgtggctt taatttgcta ggaacacagc ccaaaccacc
     8221 aaggattgaa tcatgaagac cttaatgacc tgcattttct ccctcataat attattttca
     8281 cagcctttta agcctattgt ttttgctcat actaataata ccaataatca agttttatca
     8341 gcctcggcta acaacagtgt gggcagcaac gtacccaata acatttatgt cacacctcag
     8401 gctttttgga atttatattt tgatttcacc ggagatgaaa ctccaggata tcccaaaggt
     8461 aaaattaata tttcccaaac cttatttcag tcagaaatga aaaaaaacag cagcttggcg
     8521 cagcaaaatg aaggccaatt aatcctgttt atcaactcaa cactgtatat ttataatagt
     8581 gaccgtcagc ttgagctaaa acaattgatg cgtactgcgc caaattcagg attcagtgag
     8641 atgactgcga tatcacatat tggtccagct ctgatgtatc tcgcaaaaat taaagaaaat
     8701 ggcgatgcaa gctggaaatc acaaatggaa aatttactta aggatatcca ggcagtaaaa
     8761 gtaatcaatg cacaaacacc caataattgg ctggagcaag tcaatgcgcc agcctggaag
     8821 cctcatctca ctacaatcca taatatgatt gattatgcct gttctatggc aggcaattac
     8881 atgagtgatg tattgaacga aaaattatcg tttgatatgg cctctttaca aaacgacttt
     8941 cttaatggca ataaaacgta tcctatccct tataataacg tcatgatcgg cacttttatg
     9001 ttaaccgcct tgcaaagcat ggatcagctt catagtaaaa taagccaatt aaaaatagat
     9061 tggcctcatg cgaaggtaat tatccgtttt gtcgcaggat ctaatgtttc agctggcgtg
     9121 actaaaggca gcaattggtt ggtcccattt gttcaagcac tatccaataa tacattagct
     9181 gcagatagaa tttatatcac gccttatgct gcagtaaaac catcgttagg tgctcaagag
     9241 ttaacacaaa ccgattacaa ctattataat aatacggtat ggggcgcaag acataaccgt
     9301 acaataatcg ccaatgaagt atttaccaac attacgagca tcttcttacc tgatcgccct
     9361 gctatacccg gtgattatac ttattccaaa caacctaaaa tcgaagattt tctaatgcgc
     9421 ttaaagtttt cattggcaga accaactgag atgttatcca atactgttgg gttttggatg
     9481 gctggtgaac ttgccgaaaa aaaatggaat tacaataaaa tcagcatccc gggtattact
     9541 actggtttcc cacaaggcat atccacctat ccgaacaata atcctgtaat tcagcgatga
     9601 taaacaaatt acttgtaagt actattttat tgcttatatt tcctatttca gggcaatcca
     9661 aaacggtact tgccccctat caatatttaa aaacatcggg agcaagagat ataacacctt
     9721 tcacactcga tggtgctcaa taccttgcta tacctcagtt gtcaaaagat gagccggata
     9781 ttcctgccaa tatcaacggc ggcaatgcca aagttgatgt actcattttt aagtggatta
     9841 atgggaagtt ccatttatat caaaaaatcc cggggcatgg taatgaaggc accgcttttt
     9901 ttagaataaa ggatctagcg tatctcgcta cctcctcaat ctattcaaaa ccaaaaactg
     9961 caaataacaa acatagttat accaaattat atcgttggaa tgggaggcgt ttttatccca
    10021 ttcagcaatt ttttacttat gcctccaaag gtattcttgc tttttctatt ggggaaaggc
    10081 actttttggc atttgctgat ggagtggttt tgcccaacaa taaaataaca accaatacgg
    10141 attctattat ttatgaatgg aacggtaaaa aatttattcc ttttcaatca tttcctacca
    10201 aatgggctta tggatgggac ttttttactt taaatggatc aaattacctg gcactcactg
    10261 atcatgccca agcatctcgc atttatcgct gggatggaaa aaaatttatt ttattccaga
    10321 gcttttcaaa aactggcggg cgtgcatttt cacattttac tatagatgga aaattttacc
    10381 tggcttatgc caatctcctt aacccttcta ttatttatca gtgggctggt aaccaattcg
    10441 tggaatatca acctttagag ggattgggag gacgaaattt tgtctatttt tccagccatg
    10501 gtaaacatta tctgctccga atcaatttta ttactggttc acgaataaaa ccgaaaacaa
    10561 aactgtcatc ccaactgtat caatggataa atggtaaatt taaggtggtt caaaacatca
    10621 ctacatcagg aggagttgat gcagccgttt tttcagtgaa caaccaacgc tatctgggag
    10681 ttgcaaatag tttgaatgaa cgatctcgat ttcaaacaga cagcatcatt tataaaatag
    10741 aatagcccaa aacaatgctt acaaagcaat caaattatcc tcttaaggtt tgattaatat
    10801 tcttacatta taattactca atataaccct tgagtgagga tatcaaatga aagattttgt
    10861 cattgttggg gctgggccaa taggcttata ttgggctgca agacttgcac aaataaaaga
    10921 aagttcggga ttacctatta atattgtagt tatcgaccca agggctggca attacaatcg
    10981 agaaaggata gtatccaatg aagcaatcaa atcgataagc tctcgattct caattagtat
    11041 gccatccggc aaagggatcc caagtgaggc catgtatatc agggattttg aaaaatcact
    11101 atacgaatat tgcgaaagta agggtgtaaa atttaaaaaa gggacatttt ccggcctaag
    11161 ctacaatttg gtttcgtata aagatactaa ggaaaacatc cgtacagtca aatgtgacat
    11221 ggtagttgat tgttctggtt ctaatcgtat ggttctcaaa gaaactaatc gcttgctggg
    11281 aaaagaagta tttgaaatca agacattggg taaaaaccca cataccaatc atttttcttg
    11341 ctatatgtca ttaagtactg aagaagcaaa gaaactactc atcccagacg aaatagatcc
    11401 cttgctccag gcgaaacaac tatgcaaatt acgacaaaaa tatgattggc caaatttttc
    11461 atttccagta gtcgacatac gctctgcaaa aaatgaagaa ggtggcgtta attattttat
    11521 ttattatgaa gtacccgata atttgaaaga gaaagacaag agtgtacaaa ttgaatttct
    11581 taaagattta ctcaaattga aatacggttt tgaagacatt gattttacaa ttcattcgtt
    11641 tggacatttc cctgtttgtc ccaagtatgt cgataagcca ttttatctta gtgaagactt
    11701 acctgttgtt ctggcaggtg gtgattgtca aattgaaccc gattatcgta aaggaattgg
    11761 catagaaagt ggaattgaac gtgccaattt tctatttgat actgtacatg gtacaagtaa
    11821 aggactggga ttcttatttg ataattacta tcaacaagta gccagatatg taggttatca
    11881 tggcagactg atcgaacagt tttatctgca aagagtggag aacattaatg gctcttcact
    11941 tgagcaagca aagaaaattc tttgctctgc ttgcgagtca gttaaagaag tcgaagatgt
    12001 tgctgccatt gctggcgaat taaagctttt aggcaatgaa cttttcaaga aacctaatta
    12061 tgaaagtgct ttggaatgct atcttaatgc tatccatttg tatcaatcct ttgaaaaagc
    12121 cttgccgcta acgatggatt ttgtgacatt gcattccaat gcctgccaaa cctgcctgaa
    12181 gttaaaagag tatggacaat gcataaatct cgcaaatgaa ggcatcaagg cttatgcgga
    12241 aattaaagca gaggataaag acatgctttt caaattatta ttccgaaaag cctcagccct
    12301 cgttgaacta ggtaatggtc ttgatgccaa gacacaaaga aaagaattgg atgagctatt
    12361 gaacgatctg aaagaaacat acgagctaat gcaagcaaat tcaggtgtga ataatgcgac
    12421 gttcgttaaa cagattcagt ccaaaattgt caatatcgag aaaaagcttc ctcccccaca
    12481 agaagaagta aataaaatcg aatttattta gctttaaatt ggcaaattta taaaaatgct
    12541 tggcttggtc attgatgaat agaagtctat ctaacaatga gcaagcctaa tcatttctcg
    12601 taatcgatta gaaagacggc cttccctaat aaattttcaa taaagctcaa tgctgctatt
    12661 cttaattgag atagggttgg tggagggagt gaaaatgcta aataagggat atattaaaac
    12721 aacaccatat ggattaggat tttcagagct ttttttcgaa gacaatttaa aaaaatattt
    12781 ttctgatccc gaattagaag tattgtcagc agaatactct ccttctgcca gtgataaaat
    12841 ggcgtggtct gtcaaacacc ctaaacaggg ggatcatccc ataggagtcc aacgctacaa
    12901 aataagcttg aaaaaaaacg gtaaaataca aacccgtcat gtcatcgcca aatctaaaat
    12961 tagcgataag caatatctcc agattatatc gaatgctttt gcaaaatgtg gtattcaaac
    13021 ggaaataccc atctctgatt atttgtcgca actggaattt tccaatttga acaggaaaga
    13081 aatcactatt tactatatgc aagattcgtt tgaagccttt cgacatgcta tgccattatg
    13141 ctatgggtct tatttagacg agcaagaaga aatttgtgta ttaattttag aatgccttga
    13201 tgaagaatgg ctatccctcg atccttataa tacctcgaaa tgggatcaaa cagcgattga
    13261 agcattgatt gatgcaattg cctcaatgca cgccgtctgg tatagaaaag agaaagcgat
    13321 tgccgagatc aaaggcttca aaaatccatt aaatatcgac caaatgcaac gctttatgcc
    13381 ctattggaat gccttggcaa aggcggttaa aacatcaaac ttgccttttc ttacaaagaa
    13441 agattattta ttccataccc atcttattga aactataccg caatggcaca tcaaaattga
    13501 tgctatggct aaaacattgg tacaaaatgg ctgtgtaccg aaaaatattg cattatgcaa
    13561 tcaaaatggg gtaaagaaag ttttggttta tgattgggaa attgcaacta ttcatatacc
    13621 acaacgcgat attgttgaat ttttgtctta tgtcttacct gaaaatttcg atagtaaggt
    13681 attaggtaat tatatagaga gacatcgaat aaaacttggc gaaacaacaa aacaggcgat
    13741 tgatgcaaca gaatggcgac tcggatttca atatgcttta tacgattatt taatacagcg
    13801 tgtttttcca caaatacctt ttgaaaagct tgaagctcgt aacatagcaa aagtgtataa
    13861 caatgcccgt cgcatgatgg ccttactgga ctaattttct ggagtaatta aggatgactc
    13921 atatacgtgt aatactgccc gggtacaata ctaaagtttt aaacaaggaa atgttgaaac
    13981 catttgaaag ccctggcctg gagttaagtg ttgattatgc tgacattgcg gttgatgcgg
    14041 taagacgcga atttgatatt gccttgatgg caccttatct cataaaaaaa gtaataaaag
    14101 ccgagcaaga aaaggttcat gccatagtga ttgatatgat ggcagatgtg gcaatgcgcg
    14161 cacttcgcga ggccgtgaca attcctgtcg tcagtcttgc tgaaacagca acacatgttg
    14221 cagccatgat gggcaataat tttgcgatta tcaatacaac agatgaactg acgcccattc
    14281 aggaaagttt ggtccgtagt tatgggttat atgatcggtt ggcatgtatc caaggcatac
    14341 aattggatcc gcatgacgat tatcgctcag aaaaaacaat acatggctta gttgagcaat
    14401 gtgcggatgc cgtgataaag aagcatgctg atacacttat tttcgggtca gggcgcttga
    14461 ttggttatcg tgaagttatt caaaaaggtt tagaggaaaa agggttacac atcaccatta
    14521 ttgagcccct gcctaccgga atttattttg caaaatttct ggtagactcc aaaatttccc
    14581 aaagcaaata tgtttatcgg tcacccgatg cttccacgtt aaaaacgtat tcaaaaattt
    14641 tggggaatta atcattttga ttttatctcg aatctgatta tgcctggtga ttactgataa
    14701 actttctttc catcaatata aacagcaact aacttgtttt cgcaggatct ggcaatcact
    14761 cttgccagac gatattgaag ttcatcaagg gctaactctt gtgacaaaga agaaagctct
    14821 gccagaggat agttattcct gtcatccagg ataatcaaat ccgccttttt acctgcttca
    14881 atgctgccaa tttgcttctc taaccccagc aatttagctc ctccaagagt ggcatgatat
    14941 agccagcttt ttgcatcaat cagagccgtc tttttatcaa gcctccataa tgctttatta
    15001 gtaaaactcg ctgaacgcat ttcatcaaaa agagaaagac tgtcccctcc accactgtct
    15061 gagcccaatc caacctcaat ccccaagtct ttaaaaaaag aaaccggcgc cattcccata
    15121 gcaaaagaaa aattactgga gggacaatgg ctaattccaa cttgcccagc atagtctccc
    15181 atgcgtttca tttcgtgaag tgataacaaa gtgccatgtg caaacacagt cttatcagca
    15241 atacattgtg tggattcaaa tacatcaaaa taatcatgag ctgtgctaaa caagctttga
    15301 atcaattcat caaacccttc atccttgtca agatgcgtat gcaatatcag cttgtttgca
    15361 cgggcgaact cacctgcttg cttcaacata gccgcagaac aagtcactgc aaaccgggga
    15421 gaaacagtga tatgtatatt atttttacca tggtaattgt cttttaaaaa ggtcagatct
    15481 ttaaatgctt gatcacgatc aaccagtaaa ctaccaggtg cattcctatc catcaacacc
    15541 aatccaataa acgctctgat acccatgcgc gcagccacat cgcaaactac ttctgttgct
    15601 tttcgactgc tggttaccat agtattgata gtcgttgtgc cagaacccaa acacgcatgc
    15661 ataaaaagct ccgcgtggcg ttgacatctt ttcaggtcat ttgcgaaagc tgcttctgca
    15721 ggaaaaacca aagcatttaa ccaatccatc aatggcaaat caccagatcc actaatagcg
    15781 tactgggaaa gatgactatg tgtatcaatt agacctggga ggattaaatg atcaggataa
    15841 tttgacactg tacatgatat ttcttgattt aaatgggcct gctcacccac ctcaataatg
    15901 ctatcaccat caatcaagac ataacctttc ttgataagaa gactttggcc agtttgcgta
    15961 ggagtgataa tatcccctaa aatgagatga agaatgctat tcaattcata tcctgagatt
    16021 tatttcttac tatcaaaaag tatagacaac ctcttggact aactgaaatt tggggtaaaa
    16081 aatatcatag taaaaagagc tctttctttt ccacttgggt aagagagagc tcttttgtag
    16141 gattaacaac ttgagttatt gaagcaatga gtttttattt gactctcata taaaatgaat
    16201 ctcgctttct cgcttagctg ttctttcttc tgaaattttg tccatatgct ggtgagctac
    16261 ctcttcattt cctttgccaa ggctggaaaa acgcagcgaa acgccacccg gaattaaacc
    16321 gcgattaaag aacaatccgc ggtaatctgt cgctgagtag gctttaggcc tattgacatt
    16381 gaactcaaaa gtcaagttct gcaatgcttt aataactgtt gatttggcag cattaaccaa
    16441 tcgctcttgc atttccggag taaaattctt actgtaccag cttaaatagt ccgggacatc
    16501 aatatcaaca tattcctcgg gatgagattg ggtttttaat tccgcaataa catttttcaa
    16561 gacaaaatca tgcttggaga caattttttg ctgacaaaaa ttacgttgaa agagccctgg
    16621 acctgtacca tcatttgttt tacgctcaat aatggttggt ctttttttcc gaaaagcaag
    16681 accatcaaat tcctgtcttt caccacttgt tggagaaata acctcaaaag acttgtaaag
    16741 gctcactgcc aaccgctcgg aaaaatcctt gatagtcaaa tacaactgcg gatcgctggc
    16801 tttcaggcta gaaggttgaa tagattgcat caaaccctca gcaaggtggc ttggaccact
    16861 caaaaccggt gtgctaacac ccgcccgcgg atcagaatcg ccttcttttt cttcccgttt
    16921 ttcctttcca ttatcacgtt cattcattgg cgtttctaat aaacgctcct tatcgacaaa
    16981 gatttccttg cacaattcat ctaaggtttc ttctccctca gaatcatagt caatcaactc
    17041 taccccatcc acgatttcac cctctttagg agaataataa atcaatggag aggcagtctt
    17101 gaaatcttta agactattca accagtcttg caatatttta tccactcctt cagattgacc
    17161 caactcttcc atagcttgta aatatcgcac ttgataatta cgagattcgg gaagcagggc
    17221 ttttttgtct atttcctcac ttctcttttt cacaattgct cttaatcctt ccagatattc
    17281 aatcaagggt ggaattgtct tttttctttc gctggcaaaa ttctgcctgg ctgcttcaat
    17341 tcgtttttta tagtactctt gtagatcctg ctttttgtta gcccatacac ctgttagctc
    17401 ttccctgata gcctttgttt gggtcttgcc tttcatcatg atagagttcg cattttcgac
    17461 aaactgttca tagtagtttg cataaggagt attgcctttc ggatgtgcgg ttctcagaag
    17521 acgcagttgt tgtttcattt catgaaattc ctgggcgtag agccttcttt ccagtttcaa
    17581 actttttagt ttatttttca gttgcttaat tacgatttcg cctacgattt cgtcatcacc
    17641 gctaaattca gcaattaact gtggaattct tttgatttca ttatcaattg cgttcaacct
    17701 tttctcataa aaccctagaa tttcgctgtg ttttttcaaa tcaaaaatat cagtgggctt
    17761 taaagattga aggtgcttaa gacaatgcct cataacataa gtgtaggctt taaataaatc
    17821 accattacta ttggtttgcg gatcagtcga gaaatcaatg gcctcattat ctcgtaagct
    17881 atcgcgacgt ctgtcttcca gtgatttgag ttcttttagt gctcttcgca accctcttaa
    17941 ttgatatggg atgttcttgg tttcagataa aagtgtacga actccttctg tagttctaac
    18001 cacttcatca atctctttaa tgattttgtg aaaatcttca ctcgattgag ttttcttcaa
    18061 tttgtcaatc aattgtccaa ttactttatc caaaagagca caaatcgctt gttcttctgc
    18121 caatttttca ttctttaaaa gatcaacgta taaggatttg aaatgaggtg ttacctcgct
    18181 atttctggca ggaatatccg gtaaacacag aatcgggtca ttaagattga tctggcgctc
    18241 caaatcgctt actccccctt tggctaaaac gtcattccaa tcctcattga ctgttggagg
    18301 ttctttgaca atgacgcgat aaccggcgtc aacgaatgct ttcttagcct tgtcaaactc
    18361 tgaattagcg aaaggattct ccacaggatc atcatgatct tttagtaaaa ctaccgttgc
    18421 tccaggggga tagtgagttt tgacataggc caaggtagca agcagttcag atactcccat
    18481 tgatgccaac acagaatagt tatttctgac ctcatgcaaa ctagccacac ttgccgctgt
    18541 ttcaactcct tcggcaataa aaacaacatt gacattatct gttttattaa taacagctgc
    18601 tttaccttct cgcagaggtt tggtcggtcc taaatatttc ttgcaaaaaa aatctgaatt
    18661 ttcaggattt gtcgctttgt taccagcagg gtcaatttgt attatctgca gaccaacaag
    18721 ttcctcatcc agattgtaaa cagggacaac cacataatct ttataaggtt tgtttttatc
    18781 aaactccgga ataccaatag gaccttttaa acttcgtatc tgtaattttt cggccacgac
    18841 tgccgggatt tttcgagttc ctacgatata cttttcagca ggggtgtctt ttaaaggcac
    18901 gcttcgtacc cataaatcat tggctaattt aatacttttt ttatcaataa tatatttatt
    18961 caattgatta atttctggag ttgttgtctt gcccataccg attacctcac ttaaatgaag
    19021 tgaaaatttt tgcatattta gcttaaggaa atattaaaaa aaacaacaca aaatgaatat
    19081 aaatcaatca aatgatttgc aattgctggt aaaaaataga agtgaattat cttatatcaa
    19141 attataattg tctctgatgt aaatcatgga taaaacatag gcaacgaact atgaagatga
    19201 gaacagtatt cttcatgctc ttaaaaactc aggacaaatc atccaggcaa tacaccaatc
    19261 aatcccgtct gctttatcga ggcaaacaag actgccaggt ttaaatgaaa ccaaaatgcg
    19321 atgttcctct ttcgcaacaa gttggctgac cagtttattc ataaatggca aatgccctac
    19381 aaacatcatg ttttgtttta attgattaat ctcttttgcc aatggtatga catcatcaag
    19441 cggctcaagc cctgccctgg actcaacgcc atttttgctc aaaatatgac cagacaaact
    19501 ctctgctgtt tgttgtgctc ttaattttcc actgtggtat atacaggaca cttcgatttt
    19561 aagcggggaa aggaagttgc ttaacaattg aatatctttc tgaccctgat cactcaaagg
    19621 cctttcagga tcaatatctt tatccaaact gtcaccatgt tgaacaagat aaattttcat
    19681 ctcacccttt taattcatta gctaatttat taaagtgtag actcatttca aataacagat
    19741 aagaaaagct tgtaatgaaa cctaaaattt cagtatttat tgcatcaagt ctcgacggtt
    19801 atattgcaag gaatgatggc tcaatcgatt ggcttataga agcaaattct ctcgcctctc
    19861 caggagagga ttgtggatat cgatcattta ttgccaccat agatacgata attatgggtc
    19921 gatattccta tgaaaaagtt attacatttg atgaatggcc atacggttct ataccggtaa
    19981 tagtaatgag ctcaaaaccc atacagatac cagaacactt gcagcatatc attacagttt
    20041 ctaaccaaag tcctcctgat ttagttagcg aattatccct ccatggcatt cagcatgtat
    20101 acctggatgg aggtattact atccagggat ttcttcaaaa taatcttgtt aatgagatga
    20161 tcattacatt gatacctgtt ttgattggtt caggtcgccc tttatttggc tctcttaaga
    20221 atgatatcaa actcaaacac cggttaacca ctagctatcc aggtggtttt gtccaaatta
    20281 aataccagat tggtgaatag ccagatactt tagtcctgtt tactctgtaa ttgtaatgca
    20341 gcatgcaatt gctatgttat gatggtatag tgccaattta ccagtaacag gatacgaaat
    20401 ggacattcgc tatttgatct atgcttgggg cagcagcatt ttattcttta tttatcttta
    20461 tttaagttac ctggtgatta tccaacgtcg caaatatcat gtgctctatt taaccaatga
    20521 tgataaagca tttattgccc gctttcgcgc gcatgccaac tttgctgaat acgttccgct
    20581 caccttaatc cttatgggga tgtctattat actc
//
LOCUS       DASLOE010000040        20313 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.040, whole genome shotgun sequence.
ACCESSION   DASLOE010000040 DASLOE010000000
VERSION     DASLOE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 20313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20313
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.040"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..504
                     /locus_tag="U6309_001177"
     CDS             <1..504
                     /locus_tag="U6309_001177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504627.1"
                     /translation="NKNTDNNESNRNDFVDNSFNYYKEITDDDIWIVLRRLSNEDSIA
                     GRVAHKICNRELPNSFLINPSRRFDAKKIIERYKDQEDEDNKWRIYFDELNFESYGSK
                     KIPIYVSDHNTVTDLYHSSNMLNQISDKPEPTYYVYIDAKHPKEEELLCELSSYHCLV
                     SPYFKSG"
     gene            complement(560..829)
                     /locus_tag="U6309_001178"
     CDS             complement(560..829)
                     /locus_tag="U6309_001178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504628.1"
                     /translation="MKTTKYSSNLINQLPNNKPVVYEIQTAGGNKNYIGTAKRGRVTG
                     RIAEHIGTIPGAQVKIKQFSSIQEARKSEAKQISKFQPKYNKQGK"
     gene            complement(930..2915)
                     /gene="traD"
                     /locus_tag="U6309_001179"
     CDS             complement(930..2915)
                     /gene="traD"
                     /locus_tag="U6309_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HEN5504629.1"
                     /translation="MSNYPINNLLREPTEAWSAITCLSLALLAYTQPHLFLLTQTMGL
                     YAALALLIPGIYRAWQAIKVKRYHRRLLAMPTYALSTTEIPLSKNWLFLGKGFRWRPS
                     HTQKLHQIKQVKNEKFMQRSSCYQLARRFCQNHEHTKLAKWLNTNSKLNPFRPDPPVG
                     GSPFLHGVGEEDKPVYIPQDVRVGHTFVVGTTRVGKTRLASILINQDIRNGDAVIVVD
                     PKGDQDLVRDMMAACKVSGRTEDFKIVHLGFPEQSAQYNPLKNFDQISEVATRITDAI
                     SAEGEGKQFAAFAWKYVNIVAICLEEMNQPLTYQTIAFYISRLEQLLMSYADKILPNH
                     YPNYHQEIDEIIDEHDSKIGKNNKTKSPMERHQAVIQYVKDHINKTIKNNNAEALHDQ
                     ILIDLYDAAIMDKHYYDKITASVGPVLSEINKSNASKILSSKAQPGEIELMDVIKNKK
                     VLYIGLDSLTNPNIAQAVGKAFLSDLVSTAGKIYKEPNARYTLNLHCDELSEIIQDSF
                     VKILNKAGGAGFQVTAYAQTIQDMEVALGSRAKAEVSEGNFNTLIMLRIKNEETANLL
                     VKVLPQVGVVGHTQVSMVNDTPHGDDGVYFNTTNEDRVQTSSVPMIGVDDIISLPKGQ
                     AFILINGGELFKIRIPLPNNTQYLTSKSPISLQANAI"
     gene            complement(2912..3241)
                     /locus_tag="U6309_001180"
     CDS             complement(2912..3241)
                     /locus_tag="U6309_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504630.1"
                     /translation="MIKHYIRSSIIVLIQTVLPIIALLAIAPWFINSNLLTRWQSTFT
                     TIQPLFLGLHGVLYLSLVLLWPRLILRLQNQHQITTEQLNTALKTRWYLLGIFVFIDI
                     LMIGGKL"
     gene            3256..3429
                     /locus_tag="U6309_001181"
     CDS             3256..3429
                     /locus_tag="U6309_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504631.1"
                     /translation="MRKVFRLLAVVLDAIVGVAEDKPAKCYMSVYEAQDKLDNGLITI
                     REYNEAFERKDVL"
     gene            complement(3435..4988)
                     /locus_tag="U6309_001182"
     CDS             complement(3435..4988)
                     /locus_tag="U6309_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HEN5504632.1"
                     /translation="MIVFNPLSLYTTYLGWQQYEVLFNALWQTGLLYLGFLAIGYRFL
                     KNVLNPAGAFYAVEHALNNFLYELAITFLICSLFVYPCVPLETKALQFKPLCGLKNPT
                     TAVIGDSGTTYDEAFADLLTNQVKIPIGFAIIQNFISSFTYSLMKVTGCTDSLQSIQG
                     DLVSTYLPQSVRKQALDFHRQCFIEARTQFNSEKHEASELDPMLKRYGGEDDLNWMGS
                     KILQKMYYSKLHARQPVPGFTFHQAPNRNLEKAANRGDIPPERLPEDGYPSCQQWWNK
                     IKTDLVEVSNQASVFNKHLNYYAMLDRVRQYKVKHPKAWKADISAEDFIAKMLLQESK
                     DMQTSSVQNLMDNNNGKVASAITHSLVNVGQWTKSWTSTPLKREAIMQTLPVMQAFFI
                     FFLIILTPMVLSLSCYSTRALGSLCALFIMAIFLQYLWHLVGFLERSVLDPLGENDAI
                     SAMKNMAVMFYFVAPVLLLRLSSHFGGDAGAGLMDLVNGADRQSESMAQSGMQVAKTG
                     VKIISKGIQ"
     gene            complement(4991..6358)
                     /locus_tag="U6309_001183"
     CDS             complement(4991..6358)
                     /locus_tag="U6309_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HEN5504633.1"
                     /translation="MNKYLLLTLLIPHLTHAGNFMPSQSDYYYELGGTSNLFIPPVNK
                     DQTLVIGADIDGRMGFSCSGFNPVVSITNTFQDLKKSALNIPGGVIDNLKGSVAGFPL
                     YKLQQSMPALYNVLQNAASSAQNEFTLKVKDCQEVKQALEQNQSPMEGILSVSDSQGW
                     LDAAKRAKKENVDVTETSKSIAQKRDEYGLPWIGSNKGNAGGKFQRPIKVINDVVIAG
                     YNILLERKPLDSLEKPSTKIPMVQSWPTPTDAANWAVKVLGDIQVSSSENKQSHDAKA
                     GIGLSALLQSCANANTCSQNIAKALWNLVNGTWTLTEENLSKVSASNLLITDEVITTI
                     QHLPREEQMLTISKLAEEIAVQNMLDKALMMRRILQAGLQVQEVQNLKPAMNMVLFAL
                     KKLDDDIHSLSFENDVRKKMMTETLGTIMDIRHQAQSQNMPGQDHEQPAVKNGAIYVK
                     ENKGA"
     gene            complement(6368..7342)
                     /locus_tag="U6309_001184"
     CDS             complement(6368..7342)
                     /locus_tag="U6309_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HEN5504634.1"
                     /translation="MTNTALALESTSPPHPVNTFTIATRVLQKLFQNSHYKVIGSCTW
                     TVGRLPPRLEVTPAVEQFLPDLVITVSNKPEENPWLEARTLYENKAARTMYQQAYKAA
                     TGSALGFGNDSGQTTDLHINDERTRIVDVIGSPAAFYRIPYLSHRPETGFGVPYYLAE
                     ADAVMDRTEAAELLYMGTHPHLLINHEIGTLTQHWGSEIPRLMRVTQPYNYRASVVAA
                     MHAVDIVTNKNPLHVTKSTNNSCGKNCVVANAIYDPQNNKVIWQEIYPLNRNIHPGDA
                     QDFGIEDEKAGNGNYVFVLWRKYRGCIQNKGKLVNFLTFPKVGQPQKR"
     gene            complement(7335..10085)
                     /locus_tag="U6309_001185"
     CDS             complement(7335..10085)
                     /locus_tag="U6309_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HEN5504635.1"
                     /translation="MKRLSNWLLGEMNPQAIKEQMIKKQYANPLPSFADRLAIVDFNE
                     QEQVFLFADGKSLGSGFELGDIQAEAASPEYLQAVFNKIRDTFATVVPLHAIDPWVMQ
                     MFVQDEYCLDPVIDHIKAQIPQQFIDSPLTQDYLQRLHDLYNKMTRPEGLFLDPKTDA
                     PYRGRRRRIRVLFYRQLHQTTLTREQILLEHQEVISQIETKLRSPGLEIKRLKGQDYY
                     QWWIRWFNPKSADEILEQYPYPNPMPAGFNLTQNIFFSPPESDEQGFIFEGRKQRILY
                     VDGLKEAPIIGLVSRERQQANPKHRYALLDTLPEGSIYTIQVVFSHDENLDYHLTRLE
                     KGIVGTSLKPQEVRDDIKTARNELSTGNRLFWVNQAVFYQAEDEQQAQVIEKNLHTIF
                     NDAKMPLIHSSYDIHPLNSWLNALPFNFDPHYARKYLCFDRLMFATELAALLPVYGRN
                     QGARHLPCFPFFNRLGEPVFFDLLHHDFISQNSHCAIFANSGGGKSVLTGWMIQSLLA
                     MKNARVVLFEMGNSFDRMLVHAKAHGKKTKQLLLSNKKEEAVPLNPFCEAYKAIPEIT
                     DNLSAEQAALIAQKIMDLKDPAHSEDLACGDGSRAYLAELALALRTMITEANAMEEEQ
                     FTLADETLLIEVLIDAILTSFNDGIPQMLTEHIVSAFQRRLDKETVSRKKDRILDMHD
                     RLNSYVINSAKSRFFNVPTEPLGDFDIFHIDISAIKDDTGKLALVMVSLLPRILAMAE
                     ATQNDNRPTFLFIDESHLQFQIPSVVTVCLLVAKVARKLGLWLVAITQNVTDMNSEKA
                     AKILSLIETWILLGLDEKEITDVKRFKSLTPQQETLIRDIDSQKGLYAEAVLLGSRYQ
                     GLFRVIPPRYLLALLMTEKSEKAQRHLLEKEHDVLVAAEIMAKKLEQPKATTHNRAYF
                     YD"
     gene            complement(10095..10442)
                     /locus_tag="U6309_001186"
     CDS             complement(10095..10442)
                     /locus_tag="U6309_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HEN5504636.1"
                     /translation="MVTINKRTLTMLCASLALSACSTANISQSAIPEQGLTVSQLYQQ
                     SMQQPIQKAVVQQVRFEDSGEEFKQLPNSEVPIHIFAHVAQLGDEQIIKPAYTTRFFL
                     YKQNQYALASELY"
     gene            complement(10399..11658)
                     /locus_tag="U6309_001187"
     CDS             complement(10399..11658)
                     /locus_tag="U6309_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HEN5504637.1"
                     /translation="MNKNKGIKILAGSVVFAVVMILINSRHTTSAHDDTPNPNNFHEA
                     ITSQFNDNIRDVSARLLETEKKLEQMHKENKSLQKQLKQPLQEASHTLQDELQTLKEK
                     LSALTDHQTQSYPMEGNQAPVSCQIKDLDGLLDKAPIEGKVFAEQDTSTKEEAPTKTP
                     FYTIPAGSDFSKVTLLSALMGEVPVEGKLMQPLFPFTAMVSRGDLMTANGMQLPEEII
                     GMKISGYSIGVGSFLDNISCVRAYVTAALFVFEDGHFVSIGQEQMKSSAEMVNNDSIG
                     YLTTQFGNPCIKGQYITNAPRVLAAFMAAGGIQGAGNALSKWQMSYQAEGGSAIATPT
                     GDLAHFAAGGAINEGSQKITDWLEKRIQGSFDMVFVPASIRSHSGFIPNQFSLHFSQT
                     IAIDKEHQGRVLDYGYHQQKNFDNALR"
     gene            complement(11655..12431)
                     /locus_tag="U6309_001188"
     CDS             complement(11655..12431)
                     /locus_tag="U6309_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HEN5504638.1"
                     /translation="MKRIKHILLMLGFMLTQVSYALQSEHLVWEKVPLTIELPINKER
                     LVQFPQAIKIIDQQLSTNLDILKVKGSLYLKAKDTFNDARLIVQLLPEGEVIILNLKA
                     NEKAVNTTPIEILIDDPKTTHQSGASQYEYNAIQLTRFAIQALYSPERVRNIPEGIYR
                     SPMQTNKTIPLFYGASIEAHPLASWRGGSLYVTAVDLKNLLNQPVKLQFSKLMGHWQT
                     ASFYPKGELPPRNQHESTTVFLVSDQPFATALTQHARYSR"
     gene            complement(12418..13074)
                     /locus_tag="U6309_001189"
     CDS             complement(12418..13074)
                     /locus_tag="U6309_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03746 family integrating conjugative element
                     protein"
                     /protein_id="HEN5504639.1"
                     /translation="MFEYWKKIDNLQHHNRLLLAFIGLLSVIVLALIINLMTMPKRYE
                     FWLSPNMAANGGLMKANDIPNEYVQGFVSSLMPTLYTWSNKGKEEFNQNIKAFHYYFT
                     TRHEQLLRDTLGAYKNAQLFDRSQVASLYRFMEPQDIRKIAPDTWEVKLILRITQRLK
                     DNSGMVIADKVVAYHLRVVKLNLSRLHNPFQLALDGYTRPEERLQDLLVDTEESHETH
                     "
     gene            complement(13067..13429)
                     /locus_tag="U6309_001190"
     CDS             complement(13067..13429)
                     /locus_tag="U6309_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HEN5504640.1"
                     /translation="MSQPSSRHLSHDFPAWKGLSLRELFWIVITTTPVTTLFFILLGT
                     MAGYPLAAGCIGFVIGFILSITVWPKGVARIKAGKPYGYVMKKTILWMVRLRLKHSPW
                     IHYQGQWQKNKSVGEPHV"
     gene            complement(13441..13806)
                     /locus_tag="U6309_001191"
     CDS             complement(13441..13806)
                     /locus_tag="U6309_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504641.1"
                     /translation="MKTTLRTLYLGAVNLFYAPVLLADNWFPKISDADNIGDGSKSMM
                     SVTGNYVKQGLGLLLFITAVVTFIKFITTVQHGIEESKKNEGSMVAFGTFAVMGITYL
                     AISIASGYVGYTMITKFKI"
     gene            complement(13803..14069)
                     /locus_tag="U6309_001192"
     CDS             complement(13803..14069)
                     /locus_tag="U6309_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504642.1"
                     /translation="MSKDEIARMFASTFKQASNNISAQVFSSSIRFIAISLVILAVMW
                     CVNHMMGTEEKQQQGYLLNFGSRMVRLVIGLMIFILVLFVKETT"
     gene            complement(14069..14395)
                     /locus_tag="U6309_001193"
     CDS             complement(14069..14395)
                     /locus_tag="U6309_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504643.1"
                     /translation="MKRILCLTLMIISLQCSANNPDLNETLVRIINQINAIMPLLDEA
                     QTQITPNARIQLQIEGFEDLQGQHHSGLREDLLNIRNGLIDYINQPIIAPRKIKPLEM
                     DFVRKP"
     gene            complement(14405..15037)
                     /locus_tag="U6309_001194"
     CDS             complement(14405..15037)
                     /locus_tag="U6309_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HEN5504644.1"
                     /translation="MAVKQYSRQMTMQLTIVLFLGWLAIIIWALSQWLLHGYSFAFEK
                     VNILVNTQREAITPVYQGSLLVVLPLNAKPDWVFTIPYFNKLAINDLAKDLIEKSQQF
                     VQILLLSSQCLLIKLIILFAAMPLFALTMIAGLVDGLNQRAIRTACLGRESSYVFHRL
                     NHYLKKGLVILLILWLALPVSITPAYVFVPVSLLMGLMVAMTASRFKKYL"
     gene            complement(15028..15459)
                     /locus_tag="U6309_001195"
     CDS             complement(15028..15459)
                     /locus_tag="U6309_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HEN5504645.1"
                     /translation="MIRILLLLSVALNVHSMNVIPLTLSQSDTSLKLNLSSPIGVPAK
                     SKATAGKVSRKTLDTTLLNMPLFVIGADKASIQWLEQHQEELKFMQATGFITNVNDFE
                     TIVALQEKFKLPLLPVNVDPLLAYLHEQHYPLIIAEGALWQ"
     gene            complement(15459..16280)
                     /locus_tag="U6309_001196"
     CDS             complement(15459..16280)
                     /locus_tag="U6309_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HEN5504646.1"
                     /translation="MNKLSTITLCLLLGQQAIAGLYIPGIATSPMQQADNALAKAGLT
                     EFHDEVIEEKDFLLNDVQKQEAKIWGLNEKEEKRYLQLMQSRSGVYYKDLRMTPIDIL
                     GLNARDDAEREHFASLAARQEAQKVAQNIAWNNAFYKAYNELFKDVPVVGDFDPSPYS
                     PYAHQPIQLKEGETLYLFIKPDDAVTTIVLQLIDAINRTPNTKLNLLFLDMNNNAIQL
                     WANRHQIPIQLVTNQQITLTPGNQQFEGLALAKKQTPLLLIANGKTSQVVDLGRF"
     gene            complement(16273..16860)
                     /locus_tag="U6309_001197"
     CDS             complement(16273..16860)
                     /locus_tag="U6309_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504647.1"
                     /translation="MKKFLDAKWLGLVVLNVLVIVFVCVKHQSSSINNNNLNGLEQKL
                     ITIQSQLNKPNQQPDLSPITDNIKQLGDFIQQMQNKDDHQLGDLFSTEQAAIKNQLDG
                     IKELLKHLDSQKQPIKMLPASELPFRVLSIDSVQEISVASVAYDYKTQALEKGDSLAG
                     WKILDINFAKQTIEFENADKAHVLMHLTQQEVGNE"
     gene            complement(16857..17270)
                     /locus_tag="U6309_001198"
     CDS             complement(16857..17270)
                     /locus_tag="U6309_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504648.1"
                     /translation="MLRTTIIGLLALSSLQIQAANITQVGRYATVNNQPLAAQINPLK
                     TVQQIHFPSSVQTIGEAVEYWLRYSGYHLAPQDKQNESLKQIFQQPLPQVTRNLGPLT
                     IADGLTVLVGKTLFSLKQDDLLREINFSLNARRAQ"
     gene            complement(17279..17479)
                     /locus_tag="U6309_001199"
     CDS             complement(17279..17479)
                     /locus_tag="U6309_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEN5504649.1"
                     /translation="MLILDRKIGEEIYINKGKIKITVLYEKNGLIGIGVRAPSEIDID
                     RKEVFIRKYIQKLDQENKSNQG"
     gene            complement(17473..17826)
                     /locus_tag="U6309_001200"
     CDS             complement(17473..17826)
                     /locus_tag="U6309_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504650.1"
                     /translation="MFLRFNAIYGALWLSAYNNEKALEAAKLEWADSIKEFDSQVLTF
                     AVEKIKRTQQRPPVIPVFVELCISIQKSIKAREEALRAKPENHKRTDPQIVKSHIKEM
                     MEKLTSPSVKEKKSC"
     gene            complement(17843..18742)
                     /locus_tag="U6309_001201"
     CDS             complement(17843..18742)
                     /locus_tag="U6309_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504651.1"
                     /translation="MGFLFVTTNELSALMGLPYIQQSTYLLGIRPYMDRETFLVGVKR
                     KISYQQLAEALYIEPHQGFQQSGSPSRQQLRRIIYALERAGLIKIESIGMNLIVKCLL
                     ADTDISVQNKPDTNPTQQPDTNPNIKKNYKSSSYDDFSKKSNTVENTKPDTPHNSENL
                     CVYVRAQFQKFWELYPQKQDETRAFQEFYKLKPDETLFRQMMDALQEQIENRLDMELC
                     GEWVPKWKFPANWLAQQCWNDELLPIRKQEQGHANIQTSYRKKSAADILAESCKESSF
                     SINFEDKPQTGNNVLHFNSTRSV"
     gene            18911..19165
                     /locus_tag="U6309_001202"
     CDS             18911..19165
                     /locus_tag="U6309_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN5504652.1"
                     /translation="MNMKKIIGSRITQARKANGLTIKILAERTGFGAARIGNWEQGTR
                     SPGPEEALVLSKELVVAASWLLGISNDPHGEWMDHVIFEY"
     gene            complement(19064..19270)
                     /locus_tag="U6309_001203"
     CDS             complement(19064..19270)
                     /locus_tag="U6309_001203"
                     /inference="COORDINATES: protein motif:HMM:NF012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504653.1"
                     /translation="MIETPQRAPNGYRQYLEDVVDCLQLCQTQIALKAILIFKYNMIH
                     PLSMWVIRNTKQPRSSNNQLFGQN"
     gene            complement(19359..20210)
                     /locus_tag="U6309_001204"
     CDS             complement(19359..20210)
                     /locus_tag="U6309_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504654.1"
                     /translation="MKKFAITLMSLIFINSYYPTAFSEKPNQRMMNNQSTHSLKSKNT
                     LQLSIQEIMDKKDKKLVLIKLIDTKSNKPITLNDLIEAHTQKIHLLIIDDTLLDYSHV
                     HPVETTIPGIYQFEWQPTLKSASYRAWADLIPTNTKTQEYVVADLPYPKTKKKLENPI
                     HRQPLFESSVDGYHFKLSFDKTPLHVGQAVMGKIDITDAKENPVHTLEPIMGAYAHIV
                     GFNEDFKTVTHVHPMGKEPTNNTERGGPELLFHIKPNKVGFIKMFAQVQIDGKTLFVP
                     FGVTVNS"
BASE COUNT         5543 a         3874 c         4423 g         6473 t
ORIGIN      
        1 aataaaaata ctgacaacaa tgaatctaat agaaatgatt ttgttgataa ttcttttaat
       61 tattataaag aaatcaccga tgatgatatt tggatagttt tgagaagatt gtcaaacgaa
      121 gattcaattg ctggacgagt ggcgcacaag atttgtaaca gagagttgcc aaacagtttt
      181 ctaataaatc cttcccgaag atttgatgca aaaaaaataa ttgaacgata taaagatcaa
      241 gaagatgaag ataataaatg gcgtatatat tttgatgaat taaattttga atcttatggc
      301 agtaaaaaga tacctattta tgtttctgat cacaatacgg ttacagatct atatcacagc
      361 tcaaatatgc ttaatcagat ttcagacaag cctgagccaa catattatgt atatatagat
      421 gcaaaacatc caaaagaaga agagttattg tgcgagctca gttcctatca ttgccttgtt
      481 tccccttatt ttaaatcagg ctagaaagta attttagaac ctactccagt tttgcatcta
      541 ttttgtaata atagtttttt tattttcctt gcttgttata ttttggttgg aatttcgata
      601 tttgtttggc ttcacttttg cgagcttctt gtatcgatga aaattgcttt attttaacct
      661 gagctccagg aatagtgcca atatgttcgg caatcctgcc tgttaccctg cctcttttag
      721 ctgtgcctat ataattttta ttgccacctg ctgtttgaat ttcatatacg acaggcttgt
      781 tatttggtaa ttggttgatt aaattagagc tatattttgt tgttttcatg ttacactccg
      841 ttgctaaatc atgagatctc atattgacaa aaatgatcaa aaaatgcaac aagttatgta
      901 taaaaaattg attaataatg agggtaggtt catatggcat ttgcttgcaa agagatgggg
      961 gattttgatg ttaaatactg tgtattattg ggcaatggaa ttctaatttt aaacagttcg
     1021 cctccattaa ttagaataaa tgcctgtcct tttggcaaag aaataatatc atctacccca
     1081 atcattggta cagaagaagt ttgaacccga tcctcattag tcgtattaaa ataaacccca
     1141 tcgtccccat gaggtgtatc gttaaccata gaaacctgcg tatgtccaac aacaccaact
     1201 tgaggtagca ctttaaccag taaattggct gtttcttcat ttttaatacg aagcataatc
     1261 agggtattaa aatttccttc ggatacttct gctttagccc tggagccgag tgcaacttcc
     1321 atatcctgaa tagtctgagc ataagcggtg acttgaaacc cagcaccacc tgctttattg
     1381 agaatcttca caaaagagtc ctggataatc tctgacaact catcacaatg cagatttaaa
     1441 gtatatcgtg cgttgggttc tttatagatt ttaccagctg tagacaccag atcagacaaa
     1501 aaggcttttc ctactgcttg ggcaatattg ggattggtta ggctatctaa gccgatataa
     1561 agaactttct tatttttaat tacatccatc aattcaattt cacctggttg agcttttgac
     1621 gataggattt ttgaggcatt acttttattg atttcagaca gtacaggacc gacactggcg
     1681 gtgattttgt cgtaatagtg tttatccata atggctgcat cgtagaggtc gattagtatt
     1741 tgatcgtgca gagcttcagc attgttgttt ttgatcgttt tattgatgtg gtctttgaca
     1801 tattgaatga ctgcttggtg gcgttccata ggggattttg tcttattatt tttgcctatt
     1861 ttactatcat gctcatcgat gatttcatct atctcttgat ggtaatttgg gtagtggtta
     1921 ggaagaatct tatcagcata ggacattaat aattgttcta gtcggctgat atagaaggct
     1981 attgtttggt aggtaagggg ttgattcatt tcttctaggc atatagccac gatattgacg
     2041 tatttccagg cgaaggctgc gaactgtttt ccttctccct ctgcggagat tgcatcggtg
     2101 atacgtgttg caacctcact gatttggtcg aaatttttta aggggttgta ttgtgcagat
     2161 tgttctggaa agcctaagtg tacgattttg aagtcttctg ttcggcctga tactttacag
     2221 gctgccatca tgtctctaac caggtcttga tcgccttttg gatctacaac gataaccgca
     2281 tccccatttc ggatgtcttg gttaattaga atactggcca accgtgtttt tcctactctg
     2341 gttgtaccga ctacgaaggt atgaccgaca cgtacatctt gagggatgta tacaggttta
     2401 tcttcttcgc ctacaccatg caagaatgga ctaccgccaa caggtggatc aggtcgaaaa
     2461 gggtttagtt tagaatttgt atttaaccat ttggctagct ttgtatgttc atgattttgg
     2521 cagaatcttc tggcaagttg gtagcaagag cttcgttgca tgaacttttc atttttgacc
     2581 tgtttaattt gatgcagctt ttgagtgtgg cttggtcgcc agcgaaagcc tttgcccaag
     2641 aacaaccagt ttttggataa aggtatttct gtggtggata gggcataagt gggcatggct
     2701 aataatcttc ggtgatagcg ttttacttta attgcttgcc aggctctata aattccaggg
     2761 attaacaatg cgagcgcagc gtataatccc atagtttgtg ttagtaaaaa caaatgcggt
     2821 tgagtatatg ccagcaaggc tagagacaaa caggtaatgg ctgaccaggc ttctgttggt
     2881 tctcttaata ggttattaat ggggtaattg ctcatagttt gcctccaatc atcaaaatat
     2941 caataaatac aaaaataccc agcagatacc accgagtttt taaagcggtg ttcagctgct
     3001 ctgtggttat ttggtgttga ttctgtaggc gtaaaattag ccttggccat aataagacaa
     3061 gggataaata gagtactcca tgcagaccaa gaaataatgg ttgaatggta gtgaaggtgc
     3121 tttgccagcg agtcagtaga ttgctattaa tgaaccaggg cgcaattgct aataaggcaa
     3181 tgattggtaa aacggtttga attaatacta taatggatga acggatataa tgtttaatca
     3241 tagggatagc tccaaatgag aaaggtattt cggttgttgg cagttgttct tgatgccatt
     3301 gttggagtgg cagaggataa acctgccaaa tgctacatgt ctgtttatga ggctcaagat
     3361 aagcttgata atggcttgat cactattcgt gaatacaatg aagcctttga acgtaaagat
     3421 gtgctttaac atattcattg aattcctttt gaaataattt ttactcctgt cttggctacc
     3481 tgcataccgg attgcgccat gctttcgctt tgcctatctg caccattgac caaatccata
     3541 agacccgccc cagcgtcacc gccaaaatga cttgataatc tcaacaataa aacaggtgca
     3601 acaaagtaaa acatcacagc catattcttc atggcagaaa tcgcatcgtt ttcacctaat
     3661 ggatcaagca cagagcgttc tagaaatcct actaaatgcc agaggtactg aagaaaaata
     3721 gccatgataa ataaggcgca taaactgcct aatgctctgg tgctgtagca gcttaaagac
     3781 aaaaccattg gagtgagaat gattaaaaag aatatgaaaa acgcctgcat gacgggcaat
     3841 gtttgcatga ttgcttctct ttttaatggg gtggatgtcc atgattttgt ccattgtccg
     3901 acattgacca ggctatgggt aatggcagat gcgacctttc cattgttatt atccatgagg
     3961 ttttgcacag aactggtctg catgtctttg ctttcttgca agagcatttt cgcaataaaa
     4021 tcttctgcgc tgatgtctgc tttccaggct tttgggtgtt tcactttata ttggcgaacc
     4081 ctatcaagca tggcgtagta atttaagtgt ttattaaaaa cactggcctg attagagact
     4141 tcaaccaggt cagttttgat tttgttccac cattgctgac agctgggata accatcttct
     4201 ggcaatcgtt cgggtgggat atcgccacga ttagctgcct tttcaagatt acgattaggg
     4261 gcttgatgaa aggtaaatcc tggcacgggt tggcgagcat gaagtttgct gtaatacatt
     4321 ttttgcagga ttttagaacc catccagttt aagtcatctt caccgccata gcgtttgagc
     4381 atagggtcta attcactggc ttcatgcttt tcactattga attgagttct ggcttcaata
     4441 aaacattgtc tatgaaaatc caatgcctgc ttgcgaacgc tctgggggag ataggttgat
     4501 accaaatctc cttgaatgga ttgcaaacta tctgtgcaac ccgtcacctt cataaggcta
     4561 taggtaaagc tcgatataaa attctggatg atggcaaagc ctataggaat tttgacctgg
     4621 ttagtcagta gatctgcaaa ggcttcatca taagttgtgc cgctgtcgcc aatgaccgca
     4681 gtcgttgggt ttttcagtcc gcataagggt ttaaattgta gggctttggt ttccaacggt
     4741 acacaaggat agacaaataa gctgcatatc aggaaagtta ttgccaattc ataaagaaaa
     4801 ttatttaaag catgttcaac cgcatagaac gcgccagctg gattgagcac atttttaaga
     4861 aagcgataac caatggccaa aaagccaaga tacaacagcc ctgtttgcca cagggcgttg
     4921 aatagaacct catactgttg ccagcctaga taggtggtat agagagataa aggattaaag
     4981 acaatcatga ttaagctcct ttattttctt taacgtagat tgcgccgttt ttaacagcag
     5041 gctgttcgtg atcctggcct ggcatatttt ggctttgggc ttgatggcga atgtccatga
     5101 tggttcccaa agtctcagtc atcatttttt tgcggacatc gttttcaaag gacaaagaat
     5161 gaatgtcatc gtctagtttt ttcagggcaa acaagaccat attcatagca ggttttaaat
     5221 tctgtacttc ttgcacttgt aatcctgctt gtaagatgcg gcgcatcatt aaggctttat
     5281 ccagcatatt ttgaacagcg atttcttctg ccagttttga gatagtaagc atttgttctt
     5341 cacggggcag gtgctgaata gtagtaatga cttcatcagt aatcagtaaa ttggaggcac
     5401 tgactttact cagattttct tcagtaagcg tccaagtacc attgactaaa ttccataagg
     5461 ccttggcaat gttctggcta caggtattgg cgttcgcgca gctttgcaat aaagctgata
     5521 aaccaatccc tgctttagca tcatggcttt gtttgttttc actgctgctg acctggatat
     5581 cgccaagaac cttaactgcc caattagcag catcggtagg tgttggccag ctttgtacca
     5641 taggaatttt ggtgcttggt ttttccaaag aatctaaagg ttttctctct aaaagaatgt
     5701 tgtagcccgc aatgactaca tcattaatta ccttaatggg gcgttgaaac ttaccgcccg
     5761 cattgccttt attgctgcct atccagggta gaccatattc atctcgcttt tgagcgatgc
     5821 tttttgatgt ttcggtcaca tcgacgtttt cctttttagc tcgtttggca gcatcaagcc
     5881 atccttgact atcggatact gataatatgc cttccattgg agattggttt tgttccagtg
     5941 cttgtttgac ttcctgacaa tctttaacct tgagggtaaa ttcgttttgt gcactggatg
     6001 cagcgttttg tagaacattg tataaagcag gcatggactg ttgcagctta taaagaggga
     6061 atcctgcgac tgaacctttt aagttatcaa tgacaccacc agggatattt aacgctgatt
     6121 tttttaaatc ctgaaaggta ttggtaatgg atacgacagg attaaaaccg ctacaagaga
     6181 atcccatacg gccatcaata tctgcaccaa taaccagcgt ttgatcttta ttaacaggtg
     6241 gtataaataa attggaggtt cctcctaatt cataatagta atctgattgg ctgggcataa
     6301 agtttccggc atgggttagg tgcggaataa gtaaggttaa taatagatat ttattcatgt
     6361 tgatgtccta tcgtttttga ggttgtccaa cttttgggaa agtgagaaag ttcacgagtt
     6421 tgcctttgtt ctgaatacag ccgcgatatt tgcgccaaag gacaaacacg taattgccat
     6481 ttccggcttt ttcgtcttca atgccgaaat cctgtgcatc gcctgggtgg atattgcgat
     6541 ttaaaggata gatttcctgc cagatgactt tattgttttg tgggtcgtaa atggcattag
     6601 ccaccacaca atttttaccg caggaattat tggttgactt ggtgacatgc aaaggatttt
     6661 tattggtgac aatatctacg gcgtgcatgg ctgcaacaac tgaagctctg tagttataag
     6721 gttgtgttac gcgcatcaac ctgggaattt cagaacccca atgttgtgtt aatgtgccaa
     6781 tttcatgatt aattaacaga tgaggatgcg ttcccatata taacaattca gctgcttctg
     6841 ttctatccat gactgcatca gcttcggcca gataataagg aacgccaaaa ccagtttccg
     6901 gcctgtgtga tagataaggg atacggtaaa atgcagcagg gctgccgatc acatcaacaa
     6961 tgcgcgtccg ctcatcattg atgtgaagat ctgtggtttg accgctgtca ttgccaaatc
     7021 ctaacgctga accagtcgca gctttatagg cttgttggta catcgttctt gctgctttat
     7081 tttcataaag cgttcgagct tcaagccagg gattttcttc tggtttattg ctgacggtaa
     7141 tcactaaatc aggtaaaaac tgttcaaccg caggagtgac ttcaagtctt ggcggtaaac
     7201 gtcccaccgt ccaagtgcaa gaaccaatga ctttgtaatg actgttttga aacagttttt
     7261 gcaaaacccg tgtagcgatt gtgaaggtat tcacaggatg aggtgggctt gtgctttcta
     7321 aagcaagggc agtgttagtc ataaaaataa gccctgttat gagtagttgc ctttggttgt
     7381 tcgagtttct tagccatgat ttcagcggcg accaggacat catgttcttt ctccaggagg
     7441 tggcgttgtg ctttttctga tttctcagtc attaataagg caagcaggta gcgaggtgga
     7501 atgacgcgaa acaatccctg atagcgtgag cctaataaaa ctgcttctgc atacaaacct
     7561 ttttgcgaat caatatctct gattaaggtt tcctgttgcg gtgttaagga tttaaagcgt
     7621 ttcacatcag tgatttcttt ttcatccagc cccaacaaaa tccaggtttc aattagcgat
     7681 aaaatctttg cagccttttc agaattcatg tcggtgacgt tttgggtaat tgcaaccagc
     7741 caaagcccga gctttctggc tactttggcc actaataggc acaccgttac cacagaagga
     7801 atttgaaatt gcagatggga ttcatcaata aaaagaaagg ttggcctatt gtcgttttga
     7861 gtagcctctg ccatggccag tattcttggc aacaaagaaa ccataaccaa agccagtttt
     7921 ccggtgtcat ctttaatggc agaaatatca atatgaaaga tatcaaaatc acctaaaggt
     7981 tctgttggca cattaaagaa gcgtgatttt gcgctgttaa tgacataact gtttaatcgg
     8041 tcgtgcatat ccagaattcg gtctttcttg cgagataccg tttctttatc caatcgccgt
     8101 tgaaatgcac tcacaatatg ctcagtcagc atttgtggta tgccatcgtt aaatgaagtt
     8161 agaatggcat caattaatac ttcgattagc agcgtttcat cagccagggt aaattgttct
     8221 tcttccatgg cattggcttc cgtaatcatg gtacgcaaag ccaaggcaag ctctgcaaga
     8281 tatgcacgag aaccatcacc acaagctaaa tcttctgagt gagcaggatc tttaagatcc
     8341 atgatttttt gagctattaa agcagcttgt tctgcgctta aattatctgt tatttctgga
     8401 attgctttat acgcttcaca aaatggattt aatggaacag cttcctcttt tttattgctt
     8461 aataataact gcttggtttt tttaccatga gctttggcat gaacgagcat ccgatcaaac
     8521 gagtttccca tttcaaaaag tactacacgg gcgtttttca tggcgagtag tgattgaatc
     8581 atccaacctg ttaaaactga cttaccacca ccagagttgg cgaaaatcgc gcaatgcgaa
     8641 ttttgactaa taaaatcatg gtgtagtaag tcaaagaaaa cgggttcgcc taagcggttg
     8701 aaaaagggaa agcagggtaa atgccttgca ccttggtttc ggccatagac tggaagcaag
     8761 gcagcaagtt ctgtagcaaa catcagacga tcaaaacata agtacttacg ggcatagtga
     8821 ggatcgaaat taaacggcaa cgcattgagc caggaattta aagggtgaat atcatagctg
     8881 gaatgaatta atggcatctt ggcgtcgttg aaaatggtat gcaggttttt ttctatgact
     8941 tgtgcttgtt gttcatcctc tgcttgataa aaaacagcct gattaaccca gaacagacga
     9001 ttgccagtgc ttaattcatt tcgggcagtt tttatatcat ctcttacttc ctgtggtttt
     9061 aagctggttc ccacaattcc cttttctaat cgtgtgagat gataatccag attttcatca
     9121 tggctaaaga cgacttgaat ggtgtagata cttccttctg gcaaggtatc caaaagcgca
     9181 tagcggtgtt tcggattggc ttgttgtctt tctcgggaga ctaaaccaat aatgggtgct
     9241 tcttttaagc catcaacata caggattctt tgttttcggc cttcaaaaat aaatccttgc
     9301 tcatcgctct caggcgggga aaagaatata ttttgcgtca gattaaaacc tgcgggcatg
     9361 ggattagggt agggatattg ttccaagatc tcatcggctg atttcggatt aaaccagcgt
     9421 atccaccact gataataatc ctgaccttta aggcgtttga tttctaaacc tggtgagcgt
     9481 agtttggttt caatctggct gatgacttct tgatgttcga gaagaatttg ttctctggtt
     9541 aaagtggttt gatgtagttg cctataaaat aacactctga ttcgtcgtct tcgtcctcga
     9601 tagggcgcat ctgttttagg atcgagaaat aaaccttctg gcctggtcat tttattgtag
     9661 agatcgtgca ggcgttgtaa gtaatcttgc gttaatgggc tgtcaataaa ctgttgtgga
     9721 atttgggctt taatatggtc gataactgga tcaaggcaat attcgtcttg aacaaacatt
     9781 tgcataaccc aagggtctat tgcatggagg ggaaccaccg ttgcaaaggt atcgcggatt
     9841 ttattaaaga cggcttgcaa gtattcgggg gaggcagctt cggcttggat atctcctagc
     9901 tcaaaccctg atcccaaact ttttccatcg gcaaataaaa acacctgttc ttgttcgttg
     9961 aaatcaacaa tagccagtct gtcagcaaaa gagggcaacg gattggcata ttgcttttta
    10021 atcatttgtt ctttgatagc ctggggattc atttccccaa gcaaccagtt agataatcgt
    10081 ttcatcacac ctccttaata caactctgat gccagggcat attgattttg cttataaaga
    10141 aaaaagcgtg tggtataggc tggtttgatg atttgttcat cgccaagctg agccacatga
    10201 gcaaaaatat ggataggcac ttcggaattc ggcagctgct taaattcttc gcctgaatcc
    10261 tcaaaccgta cctgttgaac aacagccttt tgtataggct gttgcatgct ttgttgataa
    10321 agctgactga ctgttaatcc ctgctcagga atggcgcttt gcgatatatt ggcggttgaa
    10381 caagcgctca aagccagact agcgcaaagc attgtcaaag ttcttttgtt gatggtaacc
    10441 ataatctaat accctccctt ggtgttcttt atcaattgct attgtttgac tgaaatgaag
    10501 actgaattgg tttggtataa aacctgagtg actgcggatg ctggcaggaa caaaaaccat
    10561 atcgaagctg ccttgtattc ttttctcaag ccaatcagta attttttggc tgccctcgtt
    10621 aatagcaccg ccagctgcaa aatgtgctaa atcacccgta ggcgtggcaa tcgcagaacc
    10681 accttcggct tgataactca tttgccattt ggataaagca ttgccagccc cctgaatacc
    10741 gccagcagcc atgaaagcgg ctaaaactct gggggcatta gtgatgtact gtcctttgat
    10801 gcaaggattg ccgaattgag tagtcagata accaatgctg tcattgttca ccatctccgc
    10861 agagcttttc atttgctctt ggccgatact gacaaaatgc ccatcctcaa agacaaataa
    10921 agcggcagtg acatacgcgc gaacgcagct gatgttatcg agaaaagaac ctacgccaat
    10981 ggagtaacca ctgattttca taccgataat ttcttccggc aattgcatcc cattggctgt
    11041 cattaagtct ccacggctga ccattgcggt aaagggaaac aagggctgca tcagtttgcc
    11101 ttcaacaggt acttcaccca tcaaggcaga taacagcgtg actttactga aatcactgcc
    11161 tgctggaatg gtatagaagg gcgttttggt tggggcttct tctttggttg aggtgtcttg
    11221 ttctgcaaag actttaccct caataggagc tttatccagt aatccatcca gatctttgat
    11281 ttggcaagat acaggagctt gattgccctc catggggtat gattgagttt gatgatcagt
    11341 aagggctgag agcttttctt tcagcgtttg caattcatct tgtagcgtat ggctcgcttc
    11401 ttgtagcggt tgctttaatt gcttttgcag cgatttattc tccttatgca tttgttccag
    11461 tttcttttct gtttccagaa gtcttgcgga cacatcacgg atgttgtcgt tgaattggct
    11521 ggtaatggct tcatgaaaat tgttgggatt aggcgtatca tcatgagcag acgtagtatg
    11581 acggctattg ataagaatca tgacgactgc gaacaccacc gatccagcta agattttaat
    11641 gcctttattt ttgttcatcg gctgtacctc gcatgttgag taagtgctgt ggcaaagggt
    11701 tgatctgaaa ccaaaaagac ggtggtgctt tcatgttgat ttcgtggagg caattcccct
    11761 ttgggataga aacttgcggt ttgccaatgt cccatgagtt ttgaaaattg cagtttcacg
    11821 ggttgattca acaggttttt caaatcgacc gcagtgacat agagactacc gccacgccat
    11881 gaagccagag ggtgcgcttc aatgcttgca ccataaaata agggaatggt tttgttggtt
    11941 tgcatgggcg agcgataaat accctctgga atatttctga cgcgttcagg cgaatacaag
    12001 gcttgaatag caaatcgggt aagctggatg gcgttatatt cgtattggct tgcacctgac
    12061 tgatgagtgg ttttgggatc atcaatcagg atttcaatag gcgtggtatt gacggctttc
    12121 tcattggctt taaggttcag tatgatcact tcaccttcgg gtaagagctg gacaatcaaa
    12181 cgtgcatcgt tgaaagtgtc tttggccttt aaatacaaac tgcctttaac ttttagaata
    12241 tcgaggttgg tactgagctg ttgatcaatg attttaatgg cctggggaaa ttgaaccagc
    12301 ctttctttat tgataggtaa ttcaatcgtt aaagggactt tttcccaaac cagatgttcg
    12361 ctttgcaggg cgtagctcac ttgcgtgagc ataaagccaa gcattaataa aatgtgttta
    12421 atgcgtttca tgggattcct ccgtgtcgac caataaatct tgtaaacgtt cttcaggacg
    12481 ggtatagcca tcgagagcga gttggaaagg gttgtgtaaa cgagacaaat tgagtttgac
    12541 tacacgcagg tgataggcta ctactttgtc agcaatcacc atgccgctgt tgtctttcag
    12601 tctttgagta atgcgcagga ttaatttcac ctcccaagta tcaggagcaa tttttctaat
    12661 atcttggggt tccatgaaac gataaagact ggctacttgg ctacggtcaa agagctgcgc
    12721 gtttttataa gcacccagcg tgtctctaag taattgttca tgacgagtgg taaaataata
    12781 gtgaaaggct ttgatgtttt ggttaaattc ttctttgcct ttattagacc aggtatatag
    12841 cgttggcatt aatgaggaca caaatccttg tacatattca ttagggatat cgtttgcttt
    12901 cattaagccg ccattggctg ccatattagg gcttagccaa aactcatagc gtttgggcat
    12961 ggtcatgagg ttaatgatta aggccaaaac gatgactgat aacaggccga taaaagccag
    13021 taataaacgg ttatggtgtt gtaggttgtc gatttttttc caatattcaa acatgaggct
    13081 ctccaacaga tttattcttt tgccactgtc cttgataatg aatccagggc gagtgtttga
    13141 gccttaatcg caccatccat agaatggttt tcttcatcac ataaccataa ggtttgcctg
    13201 ctttgatacg ggcgactcct tttggccaca cggtaatgga gaggatgaat ccaatcacaa
    13261 aaccgatgca gccagcggcc agtggatacc ctgccattgt tcctaaaagg ataaaaaaca
    13321 aggtggttac tggtgttgta gtgatgacaa tccaaaataa ttcacgcaga cttaagcctt
    13381 tccaggctgg gaagtcatgt gatagatggc gcgaagaagg ctgactcata atttcacctc
    13441 ctatattttg aacttggtaa tcatggtata acccacataa ccagaagcga tgctaatggc
    13501 taaataggtg attcccatta cggcaaaagt accgaaggcc accatggaac cttcgttctt
    13561 tttggattct tcaataccgt gttgcaccgt ggtaatgaac ttgataaagg tcacgactgc
    13621 ggtaatgaac agcaataagc caagaccctg tttgacgtaa ttgcctgtta ccgacatcat
    13681 gcttttggaa ccgtcaccaa tgttgtccgc atcagaaatt ttagggaacc agttatctgc
    13741 caataacaca ggggcgtaaa aaagattgac agctcccagg tagagcgtgc gaagcgttgt
    13801 tttcatgttg tctccttaac gaataacact aaaatgaaaa tcatcaggcc aatgacaagc
    13861 ctgaccattc gagagccgaa attcagcaag tatccttgtt gctgtttttc ctcggttccc
    13921 atcatgtggt tgacgcacca catcaccgct aaaatcacca ggcttatggc aataaaacgg
    13981 atactgctgg aaaagacttg ggctgagatg ttgttgctgg cttgtttgaa tgttgaagca
    14041 aacattctcg ctatttcgtc tttactcatt agggtttcct cacaaaatcc atttctaatg
    14101 gtttaatttt tcgaggggca atgatgggct ggttgatgta gtcaatcagg ccgtttcgaa
    14161 tgtttagcaa gtcttctctc aatccggagt gatgctggcc ttggagatct tcaaatcctt
    14221 cgatttgcaa ttggatacgg gcatttggtg ttatctgtgt ttgcgcttca tcaagcaaag
    14281 gcatgatggc gttaatctga ttaatgatac gtaccagcgt ttcattcaaa tcagggttat
    14341 tcgcagagca ttgcaggctt attatcatta aggtgaggca tagtattcgt ttcatggttt
    14401 ctccctataa gtatttctta aagcggctgg ccgtcatggc taccattaat cccatcaaca
    14461 aactgacagg cacaaagaca taagcagggg tgatgctaac gggcagggca agccacagta
    14521 tgagtaaaat caccagtcct tttttcaaat agtgattaag cctgtggaac acgtaagagg
    14581 attcacgacc taaacaagca gtgcgtatcg ctctttgatt tagaccgtca accagacctg
    14641 caatcatggt caaagcaaat aagggcatgg cagcgaatag gataatgagt tttattaaaa
    14701 ggcactggct gcttaataaa aggatctgca cgaattgctg tgatttttcg attaaatcct
    14761 tggcaagatc attgatggcc aatttgttga aataaggaat ggtaaatacc caatctggtt
    14821 tggcatttaa aggaagtact accagcaatg agccttgata aacaggggtg atggcctctc
    14881 tttgtgtgtt tacaaggata ttcacttttt caaaagcaaa ggagtagcca tgcaacagcc
    14941 attgacttaa cgcccagata ataatcgcca accaacctaa aaatagtact atggttaact
    15001 gcatggtcat ttgtcttgaa tattgtttta ctgccataaa gccccctcag caatgattaa
    15061 ggggtaatgt tgttcgtgaa ggtatgcaag caagggatca acattaacgg gcagtaacgg
    15121 tagtttgaat ttttcttgca aagcgacaat ggtttcaaag tcattgacgt tggtaatgaa
    15181 tcctgtggct tgcatgaatt ttaattcttc ttgatgctgt tccaaccatt gaatcgatgc
    15241 tttatctgca cctatgacaa acaaaggcat gtttaataaa gtggtatcca atgttttgcg
    15301 gctgactttg cctgcggtag ctttgctttt ggctggtacg cctattgggc tggacaaatt
    15361 taattttaat gacgtatcac tttgtgacag ggttaaagga atgacgttca tgctatgaac
    15421 attcaacgcg accgacagta aaagtagaat cctaatcatt aaaatcttcc taaatcgaca
    15481 acttgcgagg ttttaccatt ggctattaac agtaatgggg tttgtttctt ggcaagtgct
    15541 aagccttcaa attgttggtt gccaggggtt agcgtgattt gctgattggt cactaattga
    15601 atggggattt gatgtcgatt cgcccacagt tgtatggcgt tattgttcat atccagaaac
    15661 agcaggttaa gttttgtatt gggagtgcgg ttaatcgcgt caatcagttg cagtacaatg
    15721 gtggtaaccg catcgtcagg ttttataaat aaatagagtg tttcaccctc ttttaattga
    15781 ataggttgat gagcataggg tgagtaaggg gagggatcaa aatcgccaac cacgggtacg
    15841 tctttaaata gttcgttata ggccttataa aaagcattgt tccaagcgat attttgcgcc
    15901 actttttgtg cttcctggcg agcagcaagg cttgcaaaat gttcacgttc cgcatcatct
    15961 ctggcgttaa gtccaagaat atcaatcggt gtcatgcgca agtctttata atacacaccg
    16021 cttctgcttt gcattaattg cagatagcgt ttttcttctt tttcattcaa tccccagatt
    16081 ttagcttctt gcttttgaac atcattcagt aggaaatcct tttcttcgat cacttcatca
    16141 tgaaactcag taagacctgc ttttgctagg gcattgtcgg cttgttgcat gggtgaggtg
    16201 gcaatgccag ggatatacaa acctgcgatg gcttgctggc ctaataacag gcatagagtg
    16261 atggtagaca atttattcat tgcccacctc ttgttgagtc aaatgcatca gaacatgggc
    16321 tttatctgcg ttctcaaatt cgatagtttg cttggcaaaa ttgatatcta ggattttcca
    16381 tccggctagg gaatcccctt tctctagggc ttgggtttta taatcgtaag caaccgatgc
    16441 gactgaaatt tcctgtacgc tgtctatgct taaaactcta aaaggaagtt cgcttgctgg
    16501 cagcatttta ataggctgct tttggctatc taaatgcttc agcaattctt taatgccatc
    16561 gagctggttt ttaatagcag cttgttcggt tgaaaaaaga tcacctaatt gatggtcgtc
    16621 tttgttttgc atttgttgga tgaaatcccc aagctgtttg atattgtcag taatagggga
    16681 aagatcgggt tgttggttag gtttatttaa ttgcgattga atagttataa gtttttgttc
    16741 cagaccattc agattgttat tattgatgga tgaagattgg tgttttacac acacaaacac
    16801 aatcaccaga acatttaaaa caaccaggcc aagccatttg gcatccagaa atttcttcat
    16861 tgtgctctcc ttgcattaag actgaaattg atttctctta agaggtcatc ctgcttgagg
    16921 ctaaacaggg ttttcccaac caataccgtg agcccgtcgg ctatagttaa aggaccaagg
    16981 tttctggtga cttggggtag gggttgttgg aagatttgtt taaggctttc gttttgctta
    17041 tcctggggag caagatggta acctgaataa cgaagccaat actcaaccgc ttcacctatc
    17101 gtttgaactg agcttgggaa atgaatctgc tggactgttt taagcggatt tatctgtgct
    17161 gccaggggtt gattattcac ggtagcataa cgtccaacct gggtgatatt agcagcttgt
    17221 atttgcaggc tgcttagggc taaaaggcca ataatggttg ttctcaacat aactatccct
    17281 atccttgatt tgacttgttt tcttggtcta atttttgaat gtatttgcgg ataaagactt
    17341 ctttgcgatc gatatcgatt tcgctgggtg ctctgacacc aattcctatc aggccatttt
    17401 tttcataaag aacagtgatt ttgattttgc ctttattgat atagatttct tcaccaattt
    17461 tgcggtctag gatcagcatg attttttctc cttaactgag ggactggtta gcttttccat
    17521 catctctttg atgtgggatt taacgatttg tgggtcggtt cttttatggt tttcgggttt
    17581 agcccgcaga gcttcttctc ttgccttaat acttttttga atgctgatgc aaagttctac
    17641 aaagacagga ataacgggag ggcgttgttg cgttcttttt attttttcca ccgcaaaggt
    17701 taagacctgg ctatcaaatt ctttgatgct gtcagcccat tccagcttgg cagcttcaag
    17761 ggctttttca ttgttatagg cagaaagcca taaggcacca taaatggcat taaatcttaa
    17821 gaacaaagta tcaacccgtt tgtcatacgc ttcttgttga attgaagtgg aggacgttgt
    17881 ttcctgtttg gggtttatct tcaaagttga tgctgaagct ggattctttg caggattcag
    17941 cgagaatatc tgcggctgat ttttttctat aacttgtttg aatgtttgca tggccttgct
    18001 cctgttttct tatgggtaaa agttcatcat tccagcattg ttgggctagc caattggctg
    18061 gaaacttcca tttaggaacc cactcgccac agagctccat gtctaaacgg ttttcaattt
    18121 gttcttgcag agcatccatc atctgtctaa acagcgtttc atcgggcttg agtttataaa
    18181 actcctggaa tgccctggtc tcatcttgct tctgtggata aagttcccaa aatttttgaa
    18241 attgggcgcg cacatacaca cacaaatttt ctgagttatg aggtgtgtcg ggttttgtgt
    18301 tttcaactgt gttggatttt ttagaaaaat catcgtaact tgatgatttg taattctttt
    18361 tgatgttcgg gtttgtgtcg ggttgctgtg tcgggtttgt gtcgggttta ttttggacag
    18421 aaatatccgt atcagctaaa aggcatttta caatcaggtt catgcctatg gactcaatct
    18481 tgattaaacc agctctctcc agcgcataga tgattcttcg caattgttga cgactaggac
    18541 taccggattg ttgaaagcct tgatgaggct caatatataa agcctcagcg agttgctgat
    18601 aactgatttt tcgtttaacc ccaaccagaa aggtttccct atccatataa ggtcgaatcc
    18661 ccagcaagta ggtcgattgc tggatataag gcaatcccat cagtgcggat aattcgttag
    18721 tagtaacaaa caaaaatccc attttcttat tcatccatct aaaaacactg ttccaaattc
    18781 agtgtttgca atatcaaata atttcattta gtatcaatca ataacacgaa acgtgttgtt
    18841 gataacccat tgtaaataag cactttttag tgtcaatggg ctatttccgt ttgatatcaa
    18901 atgaacctaa atgaacatga aaaaaattat aggtagtagg attacgcagg ctagaaaagc
    18961 aaatgggttg acgattaaga tattggcaga gagaacagga tttggcgctg ccaggattgg
    19021 gaattgggag cagggtacga gaagcccagg acctgaagag gctttagttc tgtccaaaga
    19081 gttggttgtt gctgcttctt ggttgcttgg tatttctaat gacccacatg gagagtggat
    19141 ggatcatgtt atatttgaat attaagatcg cttttaatgc aatctgagtt tggcaaagtt
    19201 gaaggcaatc aacgacgtct tctagatatt ggcgatagcc attcggggct cgttgtggtg
    19261 tctcaatcaa accgtagcgt tcatacagac gaatggcaaa caggataaat cattatgcta
    19321 ggcctaaccc ctgtgaattc acaatccatc aaagatcttt atgaatttac tgtgactcca
    19381 aacggaacaa acagtgtttt cccatcgatt tgcacttggg cgaacatttt aataaatcct
    19441 actttgttag gttttatatg aaatagcaat tcaggtcctc ctcgttccgt attatttgta
    19501 ggttccttgc ccattggatg aacatgagtt actgttttaa agtcctcatt aaaaccaacg
    19561 atgtgggcat aagcacccat aatgggttct agggtatgaa ctgggttttc ctttgcatcg
    19621 gtgatgtcaa ttttgcccat gactgcttga ccgacgtgca atggtgtttt atcaaaagac
    19681 aatttaaaat ggtagccatc aacactactt tcaaataatg gttgtcgatg aataggattt
    19741 tccaatttct tttttgtctt tgggtaaggt aaatcagcaa ctacatattc ttgggttttt
    19801 gtattagtcg gtataagatc agcccatgct cgataggagg cactcttaag cgtaggctgc
    19861 cattcaaatt gatagatgcc tggtatcgtt gtttcgactg gatggacatg actgtagtca
    19921 agaagtgtat catcgataat caacaaatga atcttttgtg tatgtgcttc aataagatcg
    19981 tttaaggtga ttggtttatt gcttttggtg tcaattaatt tgattaatac cagtttcttg
    20041 tcctttttat ccattatctc ttgaatagat aattgcaagg tattttttga tttcaaagaa
    20101 tgtgtcgatt gattgttcat catgcgttga ttaggttttt cggagaaagc agtaggataa
    20161 tatgagttaa tgaatatcaa gctcattagg gtgatggcaa atttcttcac aatattttcg
    20221 tctccaagaa aaccaatagg ttttaaaata gtttcttata gttatatttt gtatgctatt
    20281 tattttaatc agtaaataga atgatatcat gat
//
LOCUS       DASLOE010000041        19447 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.041, whole genome shotgun sequence.
ACCESSION   DASLOE010000041 DASLOE010000000
VERSION     DASLOE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19447
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.041"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            235..2775
                     /gene="mutS"
                     /locus_tag="U6309_001205"
     CDS             235..2775
                     /gene="mutS"
                     /locus_tag="U6309_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN5504655.1"
                     /translation="MASSHTPMMQQYLRIKTDYPDMLLFYRMGDFYELFFDDAKRASQ
                     LLDLTLTHRGQSADKPIPMAGVPYHAVENYLARLLKKGESVAICEQIGDPATSKGPVE
                     RQVTRIITPGTVTDEALLDARKDNILLAIHTQKQKIGIAWVDLGGGRFHLQELTEEHQ
                     LNAELVRLQPAELLCKESTPLPSFCSNFAVKFRPGWEFDASNAHKLLCEQFSVTDLSA
                     FGEQNYPTALIAAGALLAYLKTTQKQSLPHLTTLTLEQSEDYLQLDASTQKHLELFEN
                     IHGGGEHCLLFILDKTACAMGSRLLKRWLGKPLKQHAIIQTRQQAIKEIIFLQQDVSL
                     HQLIKQCADVERIVSRIALKSARPRDLVSLLQTLTLLPAIHDELQENKSLLINEIKKE
                     ISPLTLLQQLLETAIIDNPPMLIRDGGVIAPGFDEELDELRNLSSNAHETLVKLEQEE
                     KNRTGLSTLKLGYNSVQGFYIELSKAQAQNAPPHFHRKQTLKNVERYITPELKLFEDK
                     VLSAQSKALAREKWLYDNLLEEIQQYIPELSDLAKSLAQLDVLVTLAERAQSLNWNCP
                     NLVPESGIMIQAGRHPVIEPLLQERFIANDLELKPNQNMLLITGPNMGGKSTYMRQTA
                     LIVLLAHIGSFVPADKVTLGPLDRIFTRIGASDDLSSGRSTFMVEMTETAQILRQATS
                     QSLVLIDEIGRGTSTYDGMALAYASCAFLASTIKAYTLFSTHYLELTELPKEFSCIRN
                     VHLQASIKTGQIVFLYRVEEGCANRSYGLEVAELAGIPKEVLKLAHEHLNQIQDTQSI
                     LVQTQIIKPPTSPVLTELKKIDPDRLTAKEALDLIYKLKHLECAESIN"
     gene            2788..4476
                     /locus_tag="U6309_001206"
     CDS             2788..4476
                     /locus_tag="U6309_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEN5504656.1"
                     /translation="MNRFAYLIILIILCFPLFAQDKKAGLFEIDGIKGKVLANVETRL
                     DELSQIKPLSQFTPTELQDQINKAIQPFGYFNAETSINNLNNKIIIKVQPGSQIRIAS
                     IKAMLTGEGAQNPLLRKTLKELPLHIGDPLFSEQYEKAKQNIINTAENMGYLHGVFKK
                     AEILIDEHKKSAQITLIFDTGPQYYFGQVQFDPTYISPQLLHRFVPFDPGQPYATDQV
                     LKLNDYLSNSGYFSSVLVKPQITDAQTVPVIVHLQPVPKYSYSFGLGYGTDTGVRGKA
                     ALHVIPVNRQGHKFNAVAQGSFRQNALQAQYVIPGKNPVTDQYALTGNFSNLNYNAGY
                     SNATLLSLSQLHNVNQFQRALSLNALYESFHYSLQPNTDQFLLYPKANITFSKTKNLL
                     FSPSGYNITFNALGANKAVLSHLNFGQLSLDAKAALTLDSLHLRLYGHTIQGITAIND
                     INELPLSLALLLGGTDNLKAYSFNSIGPGKIITYGGFEIQKEFKKNWYLVGFYDAGDV
                     YNPSVKNIQYDIGGGLMWVSPIGPIKVGLAQSVDNKMERIGHNPRLVISMGPDL"
     gene            4473..7013
                     /locus_tag="U6309_001207"
     CDS             4473..7013
                     /locus_tag="U6309_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEN5504657.1"
                     /translation="MMILKFIKKGFYYCLITAICLVSLVIFLISTTPGLYAVIKLTHL
                     YLPGTLKIHHLKGQLLDQFSIGEMEYQHQGTKIKIFNLAVNWRFSSLLHKKLPIDSMS
                     AEMIEINQKTPILILKNIKLSGYLNQQSIKLNTLQFNYWNQLITSQLELSNSFPHVFS
                     GKIRLNPHSTNKNTFSGGIDIGGNRNQIQWGGEFEGPGKITINGSLQQLKQIKQVIKW
                     RDLHWQDNKNNNLKSSQGRIEVSGTIPNLAIDLKSKLSIDQLKNWQMNASIKGKLPWQ
                     WNVDATFSQPLDFSSKLDGLYTKFALKMETQNDHHADFLITIAPGHYQMTENNLIPLI
                     QFTGGNIKGSLSQKQFSGQGVLFLDAYKKVNFNFKFPEFNVYQDIAQQTIDAKLSILF
                     NSLDFLQNASPYLINPKGQLLASIHAKGTLNKPIIESKLALNKGSFNIPALGLNITDM
                     GLAVFSKKNGWEATGSIHSSEKKLTLKGQGIFNKELGGTVSIEGSNFPIVNNKEYQIN
                     ISPQLHVRFTPSLCQITGTIQIPNAHIKPQTFTNSLVATDDIVFTSKNTQPITPLFNT
                     NMSVRVEMGEEVELTFKGLHAYLGGIVTINQLSQGPITANGELTVKKGEYKAYGQDLS
                     IEQGQLIFTGGRIDNPEINLRASKNISNSFGSVSGSSELFNFNNYNNLNVGNNFNVGV
                     EVAGRLQSPKIQLFSNPPTLSQADILSFLVIGRPANQADKAKGQLLLTAISSMNLGTG
                     TNGTQLLEQLKQKLGFDFNVQTTTNFNQQTNKISESTGVVVGKSLSERIYLSYNVGLS
                     QTDPNVLTLKYILNKFLSIQVSSSDSGNGVDVLYTKSTSSPEKAKPDK"
     gene            7047..8042
                     /gene="hemB"
                     /locus_tag="U6309_001208"
     CDS             7047..8042
                     /gene="hemB"
                     /locus_tag="U6309_001208"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEN5504658.1"
                     /translation="MNEYNLPLRLSRLRKNAMSRALVRETRLHTQQLIAPLFISEYLT
                     EAKEISAMPGQFQLGLNCIPEEIEALSKLGIPAVLLFGIPKQKDACGSESMNNNGIIQ
                     QAIKKIKSVNSDMLVITDLCFCEYTDHGHCGVLDGSQINIASTLELLGKQAVSHAEAG
                     ADWVAPSGMTDGMVLAIRQALDTASFHDIPILSYSAKYCSCLYGPFREAAQGAPQFGD
                     RKAYQMDPANSEEAVRETRLDLEEGADMIMVKPAGFYLDIIYKLKQHFADVPLCAYQV
                     SGEYSMIKNAAKNNLINEEQAMTESLIAIKRAGADFIISYFAKGMAELLNSQNFI"
     gene            8179..8571
                     /locus_tag="U6309_001209"
     CDS             8179..8571
                     /locus_tag="U6309_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504659.1"
                     /translation="MNLMLKGLSALAISLGAATTFAAPAYLTTHNRTGEESNAYIAGS
                     IPSLYPTAAYSTNQVYWNLVRLACYGHTTNGQCPALIKMATNTANPIDIGYVTMDLNT
                     GDITPKTLSAKGYSLRVIGPGEAEITKN"
     gene            complement(8869..10311)
                     /locus_tag="U6309_001210"
     CDS             complement(8869..10311)
                     /locus_tag="U6309_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEN5504660.1"
                     /translation="MKIRHKPLRTLVSTAVLGVLATGTLNAGGFSLYTEGAGYTTGNF
                     AAGVAAEAADASTGWYNPAGLVLIRDQQAVFAGVGVFPTAKLSGVSTYSTINPPPFPS
                     FVYSEVFNNINGAEDAFVPSFHYALPLGENATFGLSVVSPFGLATDWGPDSPVRYQAT
                     FTELITTNISPEIGGRLTEHVALGAGLDLQYARVKYNRILGVPNILNVLPPFNPMLAD
                     SLTYNKGHSFGVGFHVGVLGMFNDNHTRLGANYQSSMRHEFYGYSRLTGPLASPGNIL
                     APFLFPAPGIFQSDDLFSNSIDFPDVVTISAYHDVNDKLALLGSLVYTAWSSYKTVQL
                     SNAAAGRPADNGNVSQAKAISISPQNYNDTLRVAIGANYHVNDKLMLRVGGGYDPTPT
                     NNIDRDVRLPDTDRWALSAGAHYQATNNIGLDIGYTHLFTSSDPVINRTDAFTATTRY
                     NVTASGTAGANLVGVQATWTIDKEPPAPTK"
     gene            10651..13212
                     /locus_tag="U6309_001211"
     CDS             10651..13212
                     /locus_tag="U6309_001211"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HEN5504661.1"
                     /translation="MQQVVIKFGGTSVSTRATWNNIAAITQKHLNTGVQPVIVCSALT
                     QISNKLEKAIEAALLDEHHSLFNDIQSSHLNLAEQLEVNHQLIANDLHQLQQWLTGIS
                     LLKQAPAKTHAQILSLGELMMTRLGHAFLEKQGIKVKWYDARELLTSTPTLGGETMNY
                     LSARCESEYDSALVEKFLSSGAQAIITQGFFAANPHGETVLLGRGGSDTSAALLAGKL
                     QAASCEIWTDVPGIYTANPHQLPHARLLKQLNYDEAQEIASMGAKVLHPNCIPPVRKA
                     NIPMVVKYTHLPEHSGTLITKDIDESAPLIKSIQVKHSILLISIDTLNMWQQVGFLAD
                     VFAAFKKHGFSVDLLSSSEFNVTLSLDVNAKIHDRPAINALLDDLNQFGRAKLIEPCS
                     AVSLVGHHIRTVLPHLGPALEVFEAKQVYLMSLASNDLNLTFVVDESHADKLCQRLHH
                     LLIESNPQIFYYSKSWHEEFGKPNVRPTPWWEIERDRLLTTSAIHSPCYVYHSPTQAT
                     RARQLSALESIDSLFYAIKANPFPTILKTLEKEGIGFECVSIQELELVLKLFPNIKKE
                     RILFTPNFAPKSEYEFALQTGCYVTIDSLYPLENWPDLFKDREVIVRIDPGTGAGHHK
                     HVSTGGNESKFGITQNDISKILSLTKVNHIKVIGLHAHSGSGILSTDLWQQTAVMLAS
                     LTDQFPEVRSINLGGGLGIVEKPGQHPIDFAALDAQLMAVKSQYPGLAIWLEPGRFFV
                     AESGVILAKVTQCKEKGKVKFIGIETGMNSLIRPSLYGAYHEIVNLTRLHEEKAGFAH
                     IVGPICESGDTLGYDRLLPVTREGDVILIANTGAYGHCMSSHYNLRPPAQEIVLE"
     gene            13217..16447
                     /locus_tag="U6309_001212"
     CDS             13217..16447
                     /locus_tag="U6309_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEN5504662.1"
                     /translation="MRLIDSEQRSQATDPAESFIVQAPAGSGKTEILTQRFLRLLSTV
                     QSPEQIIALTFTRKAASEMRERIVQALHMAASNQPAVSAHQQSTLNFAKQALQRNNQY
                     QWDLLEQPNRLKIITIDSLCQSINRAIPLLEKQVAYPDISETPDSHYISAARCCIQYA
                     IATPEYQEAIKTLLLHVDNKQERLIQLFQALLSQRDQWLPTLFLAREQEKSTFEQALR
                     LIEQHELSRLRNSLPSDLAQELVLLSRELAVIENKPDSPRFILREWYEFQKCDQQIAG
                     ALSKLLLTSDNQLRKSFDHHVGLVKKNCAPNEFTRIKTASSDLFAKLQDCPDFLDALL
                     KVNQLPTPEYDKEQWDVLQALFLLLPLLVGHLQVSFGEHNEVDFTAISQQALAALGDL
                     DNPTDLALYLDHSIHHLLIDEFQDTSITQFELLTKLVQGWLPGEGKTLFLVGDPMQSI
                     YRFRQAEVGLFFQAKEHGIGPVRLKSLELRCNFRSTETIVNWVNDHFSKIFSQQVDIE
                     SGAVSFHPSVHVIEKNESSTIQAVQFKNREQEARHLIEIINSELNRNPEQTIAVLVRT
                     RTQLPEIISLLRQNNIPYQGTDIDLLANLVHLRDVWSLAHALLFPGNRLSWLSILHSP
                     YVGLGLSDIHCIAQYNSRKSIYHNLLHLDKIQDISEEGRIRANFFIQIMNQALLCRSH
                     SRLSDWIADTLKNLHVEKILDKAQLNDLEQLWMLLDQYEKEGRIPDMNEFLNEFNKLY
                     AQQTTPSRLQIMTIHKSKGLEFDTVILPGLGSQANRGESPMLRWLKLPTQDHGNLLLV
                     SPIKSANQDNCPLYDYLDQLDEEKSAYEAQRLLYVAVTRAKSRLYLLDHSAKSYKSSF
                     RNLLKYQEFIADPDEAQHEEQEFPLPKLVKLPIEYYQKQIPSLVQSQESSCPEISSGI
                     PRLIGIVSHQLLQWICDNHPQTINHVPWNLVHNELKALGLNQEAQQTALSMIKIQISQ
                     MYENKIGLWILSRHENEQNEYELLVEYENKLVTRIIDRTFTENGTQWIIDFKTGKEDI
                     NTLQKHRQQLNEYGRYLSDCTKFPIRCGLYYLANGHWIDWQYKMSA"
     gene            16654..17727
                     /gene="apbC"
                     /locus_tag="U6309_001213"
     CDS             16654..17727
                     /gene="apbC"
                     /locus_tag="U6309_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEN5504663.1"
                     /translation="MTIEDTVIKLIGSLKDPLLDLTGKEMNLQYKITTSNQAMHVVLT
                     AGYPTSLLETSYKPIAQKIVQDEFPNYQVTISIQQFIKAHKTQLTGKALRGVKNTIAV
                     ASGKGGVGKSTVTVNLAAALAKLGARVGILDADIYGPSIPLMLGETKPVQVKDNCYIP
                     VEAHGMQAMSIGYLTDTNQALIWRGPMLAKSLIQMLDITLWNELDYLFIDLPPGTGDI
                     QLTLVQKIPLTSAIVVTTPQNVATLDAQKAITMFSRTGIDVLGVIENMSTHICSHCGH
                     QEAIFGRGGAAALCDAYQCTLLGQLPLDSHVRRHCDEGVPTATHSSNQLTDTFIKTAM
                     RTAIELSKKPINYADRFPKIVVE"
     gene            17843..18397
                     /locus_tag="U6309_001214"
     CDS             17843..18397
                     /locus_tag="U6309_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504664.1"
                     /translation="MIRLLIITISVLCSGLTIAKSSYQIDLILFAQPQNGVKSIKSDS
                     PVPLIPVSPNAIQLHSDNNKKHMPYTLLPRSQSKLSDQHYLLSRKSQFQILGHYSWRQ
                     PAKSDSLVALPKINHNGWQIQGTLKVRQSNYYLFDADLQCLSPGNQETSFTVSQKQRL
                     KGSTIYYLDHPQLGMLVKIHKIAS"
     gene            complement(18445..19335)
                     /locus_tag="U6309_001215"
     CDS             complement(18445..19335)
                     /locus_tag="U6309_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN5504665.1"
                     /translation="MNLRDLHYFVILADVKHFGEAAKRCFVSQPTLSMQIKKLEEELG
                     VVLFERTNKQVLLTDQGSKLLDRTRKILILIDEMKELARQSEDPFTGELRLGVIPTVS
                     PYMLPLVMPELKNEYPRLKVWLIEDQTHRLITKLEQGELDVAIMALPIDKRFSCQTLY
                     EEKFYFACANTHPLAQAKSVNINDLKNQPIMLLEEGHCLREQAMAVCQSAKADDIADF
                     TATSLETLRLMVQAGMGVTLLPALSTLTASTNHLKCIPFSEPAPSRILGLFWRAGTPR
                     QVCFNAIAELITKNVQSKLV"
BASE COUNT         6387 a         3921 c         3547 g         5592 t
ORIGIN      
        1 tatgttaaat taaatgacat aaaccatgcc ttagtaatca ttttggcaaa ttctatagta
       61 atttgcagat tttaaaaatc atacttattt tttagacaca tcaaggttat atttaatgtt
      121 ttttgatcac attgtcttta ttgttttcat aaaaaccaat accaccaaca tgccttactg
      181 acttcacaca acacttttgt taaaattggg gcaatttcaa tcggctcaat cactatggct
      241 agctctcaca caccaatgat gcaacaatat ttgcgcatta aaacagacta tcccgacatg
      301 cttttatttt atcgcatggg tgatttttat gagttgtttt ttgatgatgc caaacgcgcg
      361 tcacaacttt tggatttaac tctgacacat agaggacaat ctgctgacaa acctattccc
      421 atggccggcg ttccttatca tgcggtagaa aactatttgg cacgattatt aaaaaaaggg
      481 gaatccgtag ccatctgtga acaaatcgga gatccagcta ccagcaaagg accagtagaa
      541 agacaagtaa cgcgaattat tactcctggt acagtaacag atgaagcatt attagatgcc
      601 agaaaagata atattttgtt agccatacac acccaaaaac aaaaaattgg tattgcatgg
      661 gttgatttag gtggcggtcg ttttcattta caggagttaa cagaagagca tcagttaaat
      721 gcagagttag tgcgcctgca accagcagag ctcctctgca aagaatccac gccgcttcct
      781 tcattttgtt ctaactttgc tgttaaattc agaccaggat gggaatttga cgcaagcaac
      841 gcacataaat tattgtgtga acaattctcc gtgaccgatc tttcggcctt tggcgaacaa
      901 aattacccca ctgccttgat tgcagcaggc gctttattag cttatttaaa aaccactcaa
      961 aaacagtcat taccacatct tacaacactt actcttgagc aatccgagga ttacctgcaa
     1021 ctggacgcat cgactcaaaa gcacttggaa ctttttgaaa atatacatgg tggtggtgag
     1081 cactgtttgc ttttcatatt agataaaaca gcttgtgcga tgggaagccg tttactcaaa
     1141 cgttggctgg gaaaaccatt aaaacaacat gcaatcattc aaacaagaca acaagccatt
     1201 aaggaaatta tttttcttca acaagacgtt tcacttcatc agctgattaa acaatgcgcc
     1261 gatgtggagc gcatagtatc acgtattgct ttaaaatcag cgcgcccacg tgatttggtt
     1321 tccttattgc aaactctgac actgcttcca gcaatccatg acgaattaca agaaaacaag
     1381 agcttgttaa tcaatgaaat caagaaagaa ataagccctc ttacactgct gcagcaactg
     1441 cttgaaacag ccattataga caacccgcca atgctaatta gggatggtgg agtcattgct
     1501 ccaggatttg atgaagaact ggatgagttg cgtaatttaa gcagcaatgc tcatgaaaca
     1561 ttagtaaaac ttgaacagga agaaaaaaat cgcacaggat tatccactct aaaactaggt
     1621 tacaatagcg ttcagggatt ttatattgaa ctgtcaaagg cacaagctca aaatgcgccg
     1681 ccccattttc acagaaagca aactttaaaa aatgtggaac gttatattac tcccgaacta
     1741 aagctgttcg aagacaaggt gctttcagct caatccaaag cattggctcg cgaaaaatgg
     1801 ctatatgaca atttgcttga agaaattcag caatacatac ctgagttatc ggatcttgca
     1861 aaatctctgg cgcaattgga cgtattggtc actcttgctg aaagagcaca aagtttaaat
     1921 tggaattgcc cgaatctcgt tcctgaatca ggcattatga tacaagccgg acgacatcct
     1981 gtcattgagc cattattaca agaacggttt atcgctaatg atttggaact taaaccaaat
     2041 caaaatatgt tactaatcac tgggcccaac atgggcggca aatcgaccta catgaggcaa
     2101 accgccttaa tcgttctgtt ggcacatatt ggaagttttg tacctgctga taaagtgaca
     2161 ttaggtccat tagaccgaat ctttactcgc attggggcca gtgatgattt atcctctgga
     2221 cgttccactt ttatggtgga aatgactgaa acagctcaaa tattaaggca ggcaaccagc
     2281 caaagcctcg tattaatcga tgaaattggt cgtggtacca gtacctatga tggaatggca
     2341 ttagcttacg caagctgtgc ctttcttgcg tcaacaataa aagcctatac tttgttttct
     2401 actcattacc tggagttgac cgaattaccc aaagaattct cttgtattcg caacgtgcat
     2461 ttgcaagcat ccatcaagac aggacaaatt gttttcttgt accgtgtcga agaaggttgc
     2521 gcaaatcgca gttacggatt agaagtcgca gaattagctg gcatacctaa agaagtgcta
     2581 aagctggctc atgaacatct gaatcagata caagatactc aatctatcct ggtccagact
     2641 caaattataa agcctcccac atctccagtt ttaactgaac taaaaaaaat tgatccagat
     2701 agattaactg ccaaagaagc gttggattta atttataagt taaaacacct ggagtgtgcc
     2761 gagagcatca attgatgtag ttattacatg aataggtttg catatttaat catactaatc
     2821 attttgtgtt ttccattgtt tgctcaggac aaaaaggccg ggctgtttga gattgatggt
     2881 ataaaaggaa aagttcttgc taatgtggaa actcgtttag atgaattgag ccaaattaaa
     2941 cctttaagcc aatttacccc aactgaactt caagaccaaa ttaataaagc cattcaacca
     3001 tttggatatt tcaatgcgga aaccagtatt aataatctca acaataaaat aataataaaa
     3061 gttcagcctg ggtctcaaat tcgtattgcc tccattaaag cgatgctaac aggagaggga
     3121 gcacaaaatc cattattacg aaaaactctg aaagagttac ctctgcatat tggagatcct
     3181 ctgttttcag aacaatacga aaaagcaaaa caaaatatta taaataccgc agaaaatatg
     3241 gggtatttac acggcgtatt taaaaaagct gaaatattga tagatgaaca caaaaaatcg
     3301 gctcaaatca ccctgatttt tgatacgggg ccccaatact attttggcca ggtgcaattt
     3361 gacccaacct atatttctcc tcagctatta catcgtttcg taccatttga tcctggccaa
     3421 ccttatgcca cagatcaagt tttgaaattg aatgattatt tatcgaacag cggttacttt
     3481 agttctgtac tggtaaaacc ccaaattact gacgcccaaa cagtaccggt cattgttcat
     3541 ttgcaacctg tacctaaata ttcctattca tttggccttg gctatggaac agacacagga
     3601 gtgcgtggaa aagcagctct tcatgtgatt ccagtcaatc gccaaggaca taagtttaat
     3661 gccgtagcgc aaggctcttt caggcaaaac gccttacaag cccaatatgt aatccccgga
     3721 aaaaaccctg ttaccgatca atatgccttg accggtaatt tttcaaattt aaactataat
     3781 gcaggttata gtaatgctac tctcttatcc ctgagccaat tgcataatgt gaatcaattt
     3841 caaagagcgc tttcactgaa tgctctgtat gaaagttttc attactctct acaacccaat
     3901 acggatcagt ttttgctcta ccccaaagcc aatataactt tcagtaaaac taaaaatctc
     3961 ttgttttcgc catcagggta taatattact tttaacgcgc ttggggcgaa taaggctgtc
     4021 ctctcccatc tgaactttgg acaactttca ctggatgcca aagccgcgct cacattggat
     4081 tcactgcatt tgcgcctgta tgggcatacc atccaaggta ttacagcaat caatgatatc
     4141 aatgaattac cgctttccct ggcattactt ctgggaggaa ctgacaacct taaagcctat
     4201 agttttaact ctatcggacc aggaaaaatt atcacttatg gtggctttga aattcaaaaa
     4261 gaattcaaaa aaaactggta tcttgttgga ttttacgatg ctggagatgt ttacaatcca
     4321 tccgttaaaa acattcaata cgatattgga ggcggtctga tgtgggtttc ccctattggg
     4381 ccaattaaag ttggattagc ccaatcagtc gataataaga tggagagaat aggacacaat
     4441 ccaagattgg ttatcagtat gggaccagac ttatgatgat attaaaattt atcaaaaaag
     4501 gattttacta ctgcttaata acagcaatat gtctggttag tttggttatc tttttgattt
     4561 caactacccc ggggctatat gcagtaatta aattaactca tttatatctt cctggcactc
     4621 ttaaaataca ccatcttaaa ggacaattac ttgatcaatt ttctattggt gaaatggaat
     4681 atcaacatca aggcactaaa ataaaaattt ttaacctggc ggttaactgg cgtttcagct
     4741 cattgcttca caaaaaattg cctattgata gcatgagtgc tgaaatgatt gaaataaatc
     4801 aaaagacgcc catactgata ctaaaaaata tcaaattaag cggttattta aatcaacaat
     4861 caattaaact gaatacactg caatttaatt attggaatca attgattacc agccaattag
     4921 aacttagcaa ctcatttcct catgtatttt caggaaaaat tcgtttaaac ccacattcta
     4981 caaataagaa tacattctca ggagggatag acattggcgg taatcggaat caaattcaat
     5041 ggggaggaga atttgaaggt cctggaaaaa taacaattaa cggcagtctt caacagttaa
     5101 aacaaataaa acaagtcata aaatggcgcg acttacattg gcaagacaat aaaaataaca
     5161 atttaaaaag ctcgcaagga agaattgaag tatcgggaac aattccaaat ttagctatcg
     5221 acttaaaatc caaacttagc atagatcaat taaaaaattg gcaaatgaac gcttcgatta
     5281 agggcaagct gccttggcaa tggaatgtcg atgctacgtt ctctcaacca ttagatttct
     5341 catcaaaact tgatggttta tatacaaaat ttgctttgaa aatggaaact caaaatgacc
     5401 atcatgctga ctttctgatc acgattgctc caggccatta tcaaatgaca gaaaacaatc
     5461 tgataccgtt aattcagttt actggcggca atattaaagg ctctctttcc caaaaacaat
     5521 tcagtggaca aggagttctc tttctggatg catataaaaa agtgaatttt aattttaaat
     5581 tcccggagtt taatgtctac caagatatag cacaacaaac aattgatgcg aaactatcca
     5641 ttctttttaa ctctctggat tttttgcaaa atgccagtcc ttatttaata aatcccaagg
     5701 gacaacttct cgcctccatt catgcaaagg gaactttaaa taaaccaata attgaaagta
     5761 aactcgcttt aaacaaaggt tcatttaata tccctgcttt aggtctcaat ataactgata
     5821 tgggattagc tgtttttagc aaaaaaaatg gatgggaagc gactggctct attcattcat
     5881 ctgaaaaaaa actgacctta aaaggacaag ggattttcaa taaagagctt ggtgggactg
     5941 tttcaataga aggttcaaat tttcccattg taaataataa ggaataccag attaatatat
     6001 ctccacagtt gcatgtgcgc ttcactccat cgctatgtca aatcactgga accatccaaa
     6061 ttcctaatgc ccatataaaa ccacaaacat ttaccaatag tcttgtggct acagatgata
     6121 ttgtatttac gagtaagaat actcaaccga ttaccccact ctttaatacc aacatgtcgg
     6181 tacgagttga gatgggagaa gaagttgagc tgacctttaa gggattgcat gcttatttgg
     6241 ggggtatagt tacaatcaat caattatcgc aaggccccat aacggcaaat ggtgagttaa
     6301 ctgtcaaaaa aggagaatat aaagcctatg gccaggattt atctattgaa caaggacaat
     6361 taatttttac aggcggtaga attgataacc cagaaattaa tttaagagca tcgaagaata
     6421 tatccaattc atttgggagt gtatcaggat cctctgagct gtttaatttt aataattata
     6481 ataacctcaa tgttggcaac aattttaatg ttggggtcga agtagctgga cgattacaat
     6541 cgcctaaaat tcaattattc tctaaccctc caacactttc tcaagcagat atcctatctt
     6601 ttcttgtaat aggccgccca gccaatcaag cagataaggc aaaaggccaa ttgcttctaa
     6661 cagctatttc ctcgatgaat ttaggtacag ggactaatgg aacccaattg ctggagcaat
     6721 taaagcaaaa attaggattt gattttaacg tacaaacaac aactaatttt aatcaacaaa
     6781 ccaataaaat cagtgaatca accggtgttg tggttggaaa atctctttca gagcgaattt
     6841 atttaagtta taacgtcggt ttgtctcaaa ctgaccctaa cgtattaaca ttaaaatata
     6901 tactaaataa atttttaagc attcaggtaa gcagcagtga ttcaggtaat ggcgtcgatg
     6961 tcctatatac taaaagcaca tcatcaccag aaaaagcaaa acctgacaaa taaaatctta
     7021 atccatactg tcgaaggatg taataaatga atgaatataa tttgccttta agattatctc
     7081 gtttaagaaa aaatgccatg tcaagagcat tagtaagaga gacgaggctg catacacaac
     7141 aactaattgc tccattgttt attagcgaat atttaactga agcaaaagaa atcagcgcta
     7201 tgccaggcca atttcaacta ggcctcaatt gtatacctga ggaaattgaa gctttatcaa
     7261 aattaggcat ccctgcggtc ctcttattcg gtatccctaa gcaaaaagac gcttgcggca
     7321 gtgaatcaat gaataataat gggatcattc aacaagcgat caaaaaaatt aaatccgtta
     7381 atagtgacat gttagtgatc actgatcttt gcttttgcga atacaccgat catggtcatt
     7441 gcggggttct ggatggtagt caaattaata tcgcttctac cttagagtta ttaggaaaac
     7501 aagcagtaag tcacgctgaa gcaggtgctg actgggttgc tcccagtggc atgacagacg
     7561 gaatggtttt ggctatacgc caggcattgg atactgcctc atttcatgat attcctattt
     7621 taagttacag tgccaaatat tgctcgtgcc tttatgggcc ttttagagag gcagcgcagg
     7681 gtgcaccaca gtttggcgat aggaaagcgt atcaaatgga tccggcaaat agtgaggaag
     7741 ctgtaaggga aacccgatta gatctggaag aaggcgcaga tatgattatg gttaaaccag
     7801 ccggttttta tcttgatatt atttataaac ttaaacaaca ttttgctgat gtaccgcttt
     7861 gtgcctatca ggtcagcggt gaatattcga tgataaaaaa tgcagcaaaa aataatctaa
     7921 tcaatgaaga acaagcaatg actgaaagtc tcattgcgat taaacgtgct ggcgctgatt
     7981 ttattatttc ttattttgct aaaggcatgg ctgagttatt aaactctcaa aattttattt
     8041 aattatgagt caaataaatc aaatcaagaa ctataaaacg actatataaa gagaaagact
     8101 tgcctcatct ataacaaatc tcatatagta aatgggattg gcattgccta atcaactaca
     8161 atctagaagg agaaatcaat gaatttaatg cttaaaggtt tatcggcctt agcaattagt
     8221 ctcggtgctg caacaacttt tgcagctcca gcttatttaa caacccataa cagaactggt
     8281 gaagaatcca atgcctacat agcaggcagc atcccctctc tttacccaac agcagcttac
     8341 tctaccaatc aagtgtattg gaatttagtg agattggctt gctatggaca tacaactaat
     8401 ggtcaatgcc ctgccttaat taaaatggca accaatacag ctaacccaat tgatatcgga
     8461 tacgttacca tggatctgaa tactggcgat attactccca agacgctatc ggctaaaggc
     8521 tattcgctga gagtaatagg acctggagag gctgaaatta ctaaaaacta gtcaattgtc
     8581 tccatttgtt ttgaatagct tatgtaggtt gaacgctaat acaacctaca taatcaatac
     8641 atgtaaatac ttacgtttat cttaattaag attaattatc tcagcacacg attatctaaa
     8701 cgttatttat cccgacacac ataaaactaa cagggcttat acaaaacccc aaggtcagtg
     8761 caaaaaatat ttcctaattt tagcgaagag attgggattt ttcataagtt ctgactaata
     8821 aaaaagccac gcatatcacg tggcttctaa caaggtcaag agaaagcttt atttagtcgg
     8881 tgctggaggt tctttatcaa tagtccaagt tgcttgcaca cctactaaat tggcacctgc
     8941 agttccagat gctgtaacgt tatatctggt tgttgcagta aacgcatcag tcctgttaat
     9001 aactggatca cttgaagtaa ataaatgggt atatccgata tcgagaccga tattattcgt
     9061 tgcctgataa tgagcgccag cagataaagc ccaacgatct gtatccggta aacgcacgtc
     9121 tctgtcaata ttgttcgtag gtgttggatc ataaccaccg cccacacgaa gcattaattt
     9181 atcattaaca tgataattgg caccaatcgc tacccgcaaa gtatcgttat aattctgcgg
     9241 tgatatgctt atcgccttgg cttgggaaac atttccatta tccgctggtc ttccggcagc
     9301 tgcattagag agttgtactg ttttataact actccatgca gtataaacta aagagcctaa
     9361 cagtgcaagt ttgtcattga catcatgata agcactaata gtcactacat caggaaaatc
     9421 aattgagtta ctaaataaat catcgctttg aaatatgcca ggtgccggga acaaaaatgg
     9481 cgctaatata ttacctggac ttgccaatgg ccctgttaga cgactgtagc catagaattc
     9541 atgccgcatt gatgactgat agtttgcacc gagacgagta tgattatcgt taaacatgcc
     9601 cagaacaccc acatgaaaac ctactccaaa agagtgtcct ttattgtaag tcagtgaatc
     9661 ggctaacatg gggttaaaag gtggcaaaac attcagtatg ttaggtactc caagtatacg
     9721 attgtattta actctagcat attgcaaatc caaaccagcc cccaaagcaa cgtgctctgt
     9781 taatctacca ccaatttcgg gtgatatatt ggtggtaatt aactcagtaa aagtggcttg
     9841 atagcgcact ggagaatctg ggccccaatc tgttgccaat ccaaatggtg atacaacact
     9901 taacccaaac gtagcatttt cacccaatgg caaggcataa tgaaacgagg gcacaaaagc
     9961 atcctcagcc ccattgatat tattgaaaac ctcagaataa acaaaagaag ggaaaggggg
    10021 cggattaatt gttgagtaag tactaactcc gcttaacttg gcagttggga atacaccgac
    10081 acctgcaaat acagcttgct gatcgcggat taataccaaa cctgctggat tataccaacc
    10141 tgtagaagca tccgctgctt cagcagccac accggcagcg aagttacctg ttgtatatcc
    10201 agctccttcg gtatacaaag aaaatccacc tgcgtttaat gtaccagtag ctagaactcc
    10261 gagaacagca gtactcacta gcgttcttaa gggtttgtgc ctaattttca ctccacactc
    10321 ctttcgttta ataacatccc aattcatggt aataaaaatg ataataaatg aatttataat
    10381 gatttaaaac ggtaaaaaaa aattatttcc tattttctta aatttttata aaagttttaa
    10441 tcataatgag ttaatcaagt taattatggt aaaatcaaag gcatatgata aatttttatt
    10501 cgagcctacc tttcccaacc catgctttgt agactcaact cgtgacttgg gttgtcttga
    10561 gaaaaatctt ttttaaattg gggcttaatc tacttgtaca aatactttag tccatgctaa
    10621 ggtgtattaa ttttgagcgg aatactaatc atgcaacaag tagtcatcaa atttggcggc
    10681 actagtgttt caaccagagc cacttggaat aatattgctg caattaccca aaaacatctg
    10741 aacacaggtg ttcagcccgt tatagtctgt tctgctttaa ctcaaatttc caacaaactt
    10801 gaaaaagcca tagaggcagc ccttcttgac gagcatcata gccttttcaa tgacattcag
    10861 agcagtcacc tgaatctggc agaacaatta gaagtaaacc atcaattaat tgctaatgat
    10921 ttgcaccaat tacagcaatg gctaaccggg atctccttac tcaaacaagc acctgcaaaa
    10981 acacatgcgc aaattttaag tttgggtgag ctgatgatga cccgcctggg ccatgccttt
    11041 cttgaaaaac aagggattaa agtaaaatgg tatgatgcca gagaattgtt aacaagcaca
    11101 ccaacccttg gtggagaaac aatgaactat ctctctgccc gttgtgagag tgaatacgac
    11161 tcggcattgg tagaaaaatt tctctccagt ggggctcaag ccattattac ccaaggtttt
    11221 tttgctgcca atcctcatgg tgaaaccgtt ttgcttggtc gaggaggctc tgatacttca
    11281 gcagcactac ttgcgggaaa actacaggcc gcttcttgtg aaatatggac tgatgtccca
    11341 ggcatttata ccgccaaccc gcatcaatta ccccacgccc gattattaaa gcaattaaat
    11401 tatgatgaag ctcaggaaat tgcctcaatg ggggccaaag ttttacaccc caattgcata
    11461 ccacctgttc gaaaagccaa tatccctatg gtagtcaaat acactcacct gcctgaacat
    11521 tcaggaacat tgattaccaa agacattgat gaatcggcac ccttaataaa atccatccag
    11581 gttaagcaca gcatactgct aatttctata gacactttga atatgtggca acaagtagga
    11641 tttctggctg atgtatttgc cgcctttaaa aagcatgggt tttcagttga tttgctttct
    11701 tcttctgaat tcaatgtcac cctatccctg gatgttaatg ccaaaataca tgatagacca
    11761 gcaattaatg ccttgctcga cgatttaaac caatttggac gcgccaaact gatagaaccc
    11821 tgtagtgctg tcagtttggt aggacatcac atccgaactg tattacctca tcttggcccg
    11881 gctttggaag tatttgaggc aaaacaagta tacctgatgt ctctggcctc gaatgacctt
    11941 aatttgactt ttgttgttga tgaatcacat gccgataaat tatgccaaag attgcatcat
    12001 ctactaatcg aaagtaatcc tcaaatcttt tattactcca aaagctggca tgaagaattt
    12061 ggcaaaccca atgttcgccc aacaccttgg tgggaaattg aaagagaccg cttgcttaca
    12121 accagcgcaa tacattctcc ttgttatgtt taccatagtc ccacccaggc aactcgtgca
    12181 agacaattat ctgctctgga atcaattgat agcttgtttt acgctattaa agcgaatcca
    12241 ttcccaacca ttttaaaaac tctggaaaaa gaagggatag gttttgaatg cgtttctatt
    12301 caggaattgg aattggtgtt aaaactgttt cccaatatta aaaaagaaag aattttattc
    12361 acacccaatt tcgctcccaa atccgagtat gaatttgctt tgcaaacggg ctgttatgtg
    12421 actattgata gcttataccc tttggaaaat tggcctgact tgtttaaaga ccgcgaagta
    12481 atcgttcgca tagatcctgg tacaggagct ggccatcaca agcacgtttc tactggtgga
    12541 aatgaatcca aatttggtat aacacaaaat gacataagca agattctatc acttaccaaa
    12601 gtcaaccaca ttaaggtcat aggcttacat gctcattctg gtagtggcat actctcaacg
    12661 gatttatggc aacaaactgc cgtgatgctt gcctccctaa ccgatcaatt tcctgaagtg
    12721 cgttcaatta atttaggtgg cggtctaggt attgtagaaa aaccgggcca acatcccatt
    12781 gattttgctg cgcttgacgc gcaattaatg gcagtaaaat ctcaatatcc aggattagca
    12841 atctggcttg aaccaggtcg cttctttgta gctgaaagtg gtgtcattct tgccaaagta
    12901 acgcaatgca aagaaaaagg aaaagtaaaa tttattggta ttgaaacagg aatgaactca
    12961 ttgataaggc cttccttata tggtgcgtat catgaaattg tcaatttaac tcgactacat
    13021 gaagaaaagg ccgggtttgc tcatatagtt ggcccaatct gtgagtcggg agatacattg
    13081 ggttatgacc gcttattacc agttaccaga gaaggcgatg tgatattaat tgcaaacact
    13141 ggagcctatg gtcactgtat gagttcacac tacaatttaa gaccacctgc tcaggaaata
    13201 gttctggaat aatggcatgc ggttaattga tagtgaacaa aggagccaag cgacagatcc
    13261 tgctgaatca tttatagtcc aggctcctgc cgggtcagga aaaacagaga ttcttaccca
    13321 acgcttttta cgtttgctaa gcactgtgca gtctcctgaa caaattatcg ccttgacttt
    13381 tacccgcaaa gcagcaagtg aaatgagaga acgaatcgtt caggcattac atatggccgc
    13441 gtcaaatcaa ccggctgtca gcgctcatca acaaagcact ttaaattttg caaaacaggc
    13501 tttacagcga aataatcaat atcagtggga tttacttgag caaccaaatc gcttaaaaat
    13561 cataaccatt gattcattgt gccaaagcat taacagggca attcctttat tggaaaaaca
    13621 ggtggcttat ccagacattt ccgagacacc cgattctcat tatattagtg ctgcacgttg
    13681 ttgtatccag tatgctattg ctacgccaga gtaccaggaa gcaattaaaa ccttgcttct
    13741 tcatgttgat aataaacagg aacgattaat tcaattattc caggcacttc ttagccaaag
    13801 agatcaatgg ttgccgacat tatttctggc acgtgaacaa gaaaagtcaa cttttgagca
    13861 agctttacgc cttattgaac agcatgaact ctctcgtcta agaaatagcc tgccatcgga
    13921 cttggctcaa gaactagtgc ttttgtcgcg tgaattagcc gttatagaaa acaaacctga
    13981 ttcaccacga tttatattaa gagaatggta tgagtttcaa aaatgcgatc agcaaattgc
    14041 aggtgcttta agcaaattac tcttaactag tgacaatcaa ttgcgtaaat cgtttgatca
    14101 tcatgtggga ttagttaaaa aaaattgtgc accaaatgaa tttaccagaa taaaaacagc
    14161 cagttcagat ttgtttgcta aacttcaaga ttgtcctgat ttcctggacg cgctccttaa
    14221 ggtcaatcaa ttaccaactc ctgaatacga caaagagcaa tgggacgttt tgcaagcact
    14281 ctttttgtta ctgccattat tggttggaca tttacaagtt tcatttggtg aacataacga
    14341 agttgatttt actgccatct ctcaacaagc cttggcagct ttgggtgatt tggataatcc
    14401 gactgatttg gcactctatc tggatcattc aattcatcat cttctaatag atgagtttca
    14461 agatacctcc attacacaat ttgaattact taccaagtta gtccaaggat ggttacctgg
    14521 agagggaaaa accttattcc ttgtaggcga tcctatgcaa tccatctacc gtttcagaca
    14581 ggctgaagtt ggattgtttt ttcaggcgaa agagcatggc ataggaccag ttcgcctcaa
    14641 atcattggaa ctaagatgta atttccgttc gacagaaaca atagttaatt gggtaaatga
    14701 tcatttcagt aaaatatttt ctcaacaagt tgatatcgaa tcaggagcag tatctttcca
    14761 tccttctgtt catgtcatcg agaagaatga atcaagcact attcaggccg tgcaatttaa
    14821 aaatcgtgaa caggaagcaa ggcatttaat tgaaataatt aatagtgaat taaaccgcaa
    14881 ccctgaacaa actatcgcag ttttagtgag gacaagaacg caactccctg aaatcattag
    14941 cctacttcgc caaaataata tcccttatca gggaacagac atcgatttac tagccaattt
    15001 agtgcattta cgtgacgtct ggtcacttgc tcatgccctg cttttcccag gaaatcgatt
    15061 gtcctggtta tcgatactcc acagccctta tgttggattg ggattatctg acatccactg
    15121 catagcccaa tataattcaa gaaaatcaat ttatcacaat ttgctccatt tagataaaat
    15181 tcaggatata agtgaagaag gcaggattag agccaatttt tttattcaaa tcatgaacca
    15241 ggctttgtta tgtcgatcac atagtcgtct atctgattgg atagcggata ccttaaaaaa
    15301 tcttcatgta gaaaagatcc ttgataaagc tcagttaaat gatctggagc aattatggat
    15361 gttattagac caatatgaaa aagaaggtcg aattcctgat atgaacgaat ttttaaatga
    15421 attcaataaa ttgtatgccc aacaaaccac cccttcccgg ttacaaatca tgactattca
    15481 taaatccaaa ggactggaat tcgatacggt tattctacct ggccttgggt cacaagcaaa
    15541 tcgtggtgaa tctcccatgt tgcgctggct taagctgcct acccaagacc atggcaactt
    15601 gttactggta tcacccataa aaagtgcaaa ccaggataat tgccctttgt acgattatct
    15661 ggatcaattg gatgaagaaa aatccgccta tgaggcgcaa agattacttt atgttgctgt
    15721 tacccgggca aaatcaagat tatatttgct tgatcattcg gcaaaatcct ataaatcttc
    15781 ctttcgaaac ttattaaaat accaggaatt cattgcagat cctgacgaag cccaacatga
    15841 agaacaagaa ttccctctgc cgaaattagt caaactacct atagagtatt atcaaaaaca
    15901 gattcccagt ttagttcaat ctcaagagag ttcatgccca gagatatctt cgggtattcc
    15961 tcggctaata ggcattgtgt cccatcaact attacaatgg atttgtgata accatcccca
    16021 aacaattaat catgtgccat ggaacttggt acataatgaa cttaaagcat taggattaaa
    16081 ccaggaagcc caacaaactg ctctatcaat gataaaaatc caaatttctc aaatgtatga
    16141 aaataaaatt ggcctctgga ttttatccag acatgaaaat gaacaaaatg aatatgagct
    16201 tctggttgag tatgaaaata aattggttac ccgtattatc gatcgtactt ttactgaaaa
    16261 tggcacacaa tggatcattg attttaaaac cgggaaagaa gatataaaca ccttacagaa
    16321 gcatcgccaa caattaaatg agtacgggcg ctatctgtca gattgcacaa aattcccaat
    16381 tcgatgtgga ttgtattatc ttgcaaatgg acattggatt gattggcagt ataaaatgag
    16441 cgcttaaaaa cacagcagct atgcaattgc ccaataaaat caaatgacat tatcttaagc
    16501 tattcgtctt actcatcaga aaagctatac ccaaaataca tttctctcaa gtagatgcat
    16561 tcatcaaccc agcaaaaacg aatagtaatg gtactagtga ttagccgaga cctaagttat
    16621 cattacctat ttattttcat accaggctaa aaaatgacta ttgaagatac agtaattaag
    16681 ttaattggct cattaaagga tcctttgctt gatcttactg gcaaagaaat gaatctacag
    16741 tacaaaataa caactagcaa ccaggctatg catgttgtct tgactgccgg atatcctacc
    16801 tctttgttag aaacgagcta taaaccaata gcacagaaaa tagttcaaga tgaattcccc
    16861 aattaccaag ttactatttc tattcagcaa ttcataaaag cgcataaaac tcagttgaca
    16921 ggcaaagccc tgcgaggagt aaagaatact atcgcagtgg cctctggcaa aggaggggtc
    16981 ggaaagtcta ctgtcacagt aaatcttgca gcagcattgg caaaactggg tgctcgagtc
    17041 ggcattttgg atgcggatat ttatggacca agcataccat tgatgcttgg tgaaacaaaa
    17101 cctgtacaag taaaagataa ttgctacata ccggttgaag ctcatggcat gcaggccatg
    17161 tctattggct acttgaccga tacaaaccag gcattaattt ggagaggtcc tatgctcgcc
    17221 aagtctttaa tccaaatgtt agacatcact ctttggaatg aattggatta tttattcata
    17281 gatctaccac ctggcactgg agatattcaa ctcactttgg tacaaaaaat tcctttaacc
    17341 tctgccatag tagttactac tcctcaaaat gtcgcaactt tagatgccca aaaggcaata
    17401 accatgtttt ccagaacagg cattgacgta cttggagtaa tagaaaatat gtccacccac
    17461 atttgtagcc attgtggtca tcaagaagca atctttggta gaggtggtgc agctgcgctg
    17521 tgtgacgcat atcaatgtac attattaggt caactgccgc tggacagcca tgtacgtaga
    17581 cattgcgatg agggagttcc cacggcaaca cattcatcaa atcaattaac tgacaccttt
    17641 attaaaacag cgatgcgaac tgcaattgag ctcagcaaga aacccataaa ctatgctgac
    17701 agatttccaa agatagttgt ggaatagaag taatagcttc tctactatgt attattccct
    17761 tcagatgagt atataataca taaaaattga gtttgccacc tcaaagataa ccaagctcac
    17821 aaaaaacaaa aataaaaatg tcatgattag attattaata ataacaatca gtgtattatg
    17881 ttcgggactt acgatagcca aatcaagtta ccaaattgat ttaatactct ttgcacaacc
    17941 acaaaacggt gttaaaagca taaaatcaga ttcgcctgtt cccctcattc ctgtcagtcc
    18001 aaatgctatt caactgcatt cggataataa taaaaaacat atgccttaca ctcttctacc
    18061 gcgatcgcaa tcaaaactaa gtgatcaaca ttatctttta agccgtaaat cccaatttca
    18121 aatcctgggt cattattcct ggagacaacc agcgaaaagt gacagtttgg ttgcgctacc
    18181 taaaatcaac cataatggat ggcaaattca aggtaccctc aaagtgcgtc aaagtaatta
    18241 ctatctattt gatgctgacc tgcagtgttt gtctccaggt aatcaggaga cttcttttac
    18301 tgtttcgcaa aaacaacgct taaaaggaag cacaatttat tatcttgatc atcctcagtt
    18361 aggaatgcta gttaaaattc ataaaatagc ttcatgaaag ctcatccagc taagcaggaa
    18421 tttacgtata atttataata atttctatac taatttggat tgaacatttt ttgttattaa
    18481 ttcagcaatt gcattaaaac aaacttgtcg tggcgttcct gctcgccaaa ataatcccaa
    18541 gatacgagag ggtgccggct cagaaaaagg aatacatttt aaatgattag ttgaggcagt
    18601 tagtgtagac aaagcgggca gtaaagtgac gcccattcca gcttgtacca ttaatctcaa
    18661 ggtttccaaa ctggtggcag taaaatctgc aatgtcgtca gctttcgctg attggcatac
    18721 ggccatggct tgctccctca ggcaatgccc ttcttctagc aacataatcg gctgattttt
    18781 taaatcattg atattaactg atttggcttg ggccaaagga tgagtatttg cgcaagcaaa
    18841 atagaacttt tcttcatata aagtttgaca tgaaaaacgc ttatctattg gaagtgccat
    18901 gatagcaaca tccagttctc cttgctccaa tttagtaatt aacctatgag tttgatcctc
    18961 tattaaccaa actttcaggc gagggtactc atttttaagt tcgggcatta ccaagggcaa
    19021 catgtaaggc gatacggtag gtataacccc caaacgcaat tcacctgtaa atggatcttc
    19081 cgactgacga gcaagttctt tcatttcatc aatcaggatt aatatttttc tagttcgatc
    19141 taataatttg gagccttgat cagtcagtaa aacctgtttg ttggtacgct caaataacac
    19201 aacacctaat tcttcttcca gcttttttat ttgcatactc aacgtgggct gacttacaaa
    19261 acacctctta gcggcttcac caaaatgctt tacatcagcc aggatgacaa aataatgtaa
    19321 atctcttaaa ttcataattg ctcaataata aaaaatctat tgtgacacaa attacaaatc
    19381 tgcagtataa aaatcatttt tcaaatttta cgaaatacat acagaattat tgattccaat
    19441 gtaatag
//
LOCUS       DASLOE010000042        19115 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.042, whole genome shotgun sequence.
ACCESSION   DASLOE010000042 DASLOE010000000
VERSION     DASLOE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 19115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19115
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.042"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(38..247)
                     /locus_tag="U6309_001216"
     CDS             complement(38..247)
                     /locus_tag="U6309_001216"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504666.1"
                     /translation="MHQWISWWTRTANDWTKHNLLTWFIDTCWDASAKEVARMLLHRL
                     DRAQAALANSDHSYVNQALVLVQAQ"
     gene            complement(563..682)
                     /locus_tag="U6309_001217"
                     /pseudo
     CDS             complement(563..682)
                     /locus_tag="U6309_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            complement(977..4129)
                     /gene="putA"
                     /locus_tag="U6309_001218"
     CDS             complement(977..4129)
                     /gene="putA"
                     /locus_tag="U6309_001218"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEN5504667.1"
                     /translation="MLEKQSIHLPEGLRAAINKAYRMDELSLITELSEQAALDPQQMM
                     AIKTSATKLVQSVRSERKKSTGIDSFLTEYALSSDEGIALMCLAEALLRVPDNATIDN
                     LIKDKLAGGDWGAHRGQSESFFVNATTWALMLTGKVLTPEKAENTLTKALLKLVNRSS
                     EAVVRKAVDKAMRIMSKQFVMGRTINEALARAKKKEDRGYRYSYDMLGEAALTSADAA
                     RYFEAYKEAIISIGEKADKHSDVYRRPGISIKLSALHPRYSEFQYERVMAELPPKLLA
                     LSRLAKDYGIALTIDAEESERLDLSLDVIEKVFTDESLQGWNGFGLAVQSYQKRAFYV
                     LDWVAALARSKQRRIMVRLIKGAYWDSEIKKTQMQGFSEYPVFTRKVFTDVSFQACAK
                     KILTMTDAIYPQFATHNAYSVAMILNLVGGYRDFEFQCLHGMGNELYEQIVPANCYGI
                     PCRIYAPVGSHEDLLPYLVRRLLENGANSSFVNRIVDDKAPISELVEDPVAKSRSLLD
                     KINKNIPLPEDIFLPVRKNSKGFDFTNRLERALLQQELAKIESKEWQASPMIAGRKLS
                     RDLLQTVMSPQQPAYAIGSVQQATLDDVEVALNQAKLAFELWSKKPVEERASCLNRFA
                     DLLQANMAELMVLTCREAGKTWSDGIAEVREAIDFCRYYAKKAQELMSSPQRFNGYTG
                     ELNELSLHPRGTILCISPWNFPLAIFTGQVVAGLVTGNCVIAKPAEQTPLIAAYAVKL
                     MHQAGIPEGVIQLIPGAGETIGAALVADKRIKAVLFTGSTDTANLINRTLATRGGEII
                     PLIAETGGQNAMIVDSSALLEQVVVDAVTSAFGSAGQRCSALRVLYVQEEVYPRTVEL
                     LKGAMAELVVGDPQWLSTDVGPVIDKEALSILKNHVENMRKHHEILYQCTVDDEALSG
                     YFMPPTAIAIDSISALEKEVFGPILHVIQFKRKDLDKVINQINQTGYGLTLGIHSRIN
                     ETVDYIRQRVHAGNCYVNRNMIGAVVGLQPFGGEGLSGTGPKAGGPNYLIRLCHERTY
                     TVDTTAAGGNASLMSIPEEG"
     gene            4686..5363
                     /locus_tag="U6309_001219"
     CDS             4686..5363
                     /locus_tag="U6309_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEN5504668.1"
                     /translation="MNTLFTLISTVLLTSNITSSTPLFAKPGTDINTQVQHLSHKAPQ
                     LNKKVLKLALTAYKNASKRGAVKKPVLTVIDYSLPSNKQRMWVFDLRNERLLYNTYVA
                     HGKNSGVNVARSFSNRESSKQSSLGTYITKNTYIGHKGYSLNLQGLDRGFNDNAYNRR
                     VVIHGAWYVEPEFIKKAGRAGLSWGCPAIAQTLAKPVINTIKNGSVVFAYYPDKKFLS
                     HSGYLVA"
     gene            5795..6160
                     /locus_tag="U6309_001220"
     CDS             5795..6160
                     /locus_tag="U6309_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HEN5504669.1"
                     /translation="MNQQLNATKKDKLQVIDWLIENFPNAFFKKGNQVKPLKIGIFDD
                     LIDFYERLDTPPFSKKSLREALSYYSASPAYLSCQKPDTARVDIYGNEVDVVTPEQAK
                     YAYQRYQERYGNKKSQELK"
     gene            complement(6188..6880)
                     /gene="ubiG"
                     /locus_tag="U6309_001221"
     CDS             complement(6188..6880)
                     /gene="ubiG"
                     /locus_tag="U6309_001221"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEN5504670.1"
                     /translation="MNQIESTIAIEEVHKFAQLANDWWDTNGPLRTLHDINGARFEFI
                     SEHINLKGLRVLDVGCGGGILCESMAKAGAYVSGLDAEPEAIQIAKEHAHKNQLQIDY
                     FCNPIEEYEEQGFDVITCMELLEHVQNPELVLQHCRRLLKPNGLLFLSTISRTLKAYL
                     GAIIAAEYVLNLLPRQTHDYDKFIKPSELVKMARLYDLNLIDMKGLCYNPFLRKTTLV
                     SDVSINYIMVLQ"
     gene            complement(6867..7586)
                     /locus_tag="U6309_001222"
     CDS             complement(6867..7586)
                     /locus_tag="U6309_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN5504671.1"
                     /translation="MYENLLVGCHEEWKEILMKALNTMDSDYLKQIQEDSHWLPGLNQ
                     LFSAFSLPLSQTQYILLGESPYPRKISANGYAFWDNSVGLLWSSTGLSKEVNRATSLR
                     NWIKTLLIARGDLHGNTSQEAIAKINKSCLVQTAEDFFKGMMKKGILLLNASLIYSKG
                     KVPYHARHWKPFMQNLFEQLAIRKPDVQLILFGKIAEKIPANKLLIGLVSEHPYNVSF
                     ITNQRVIEFFKPMDLLSYESN"
     gene            7850..9532
                     /gene="legC3"
                     /locus_tag="U6309_001223"
                     /gene_synonym="ppeA"
     CDS             7850..9532
                     /gene="legC3"
                     /locus_tag="U6309_001223"
                     /gene_synonym="ppeA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector
                     LegC3/PpeA"
                     /protein_id="HEN5504672.1"
                     /translation="MIMFLANCNIEELVTEHIKQFLADEELSFSGLKDLILSKAPIPW
                     IHSSVTATLLKSRDSDKTEVKKNLEQQSYKAQLAEDKIQKEQDDAEALKDKKLKEVLT
                     RELNHIPTQISEQQTELRLLHYKLERLFESQAKVDVIQHSDSPMKKIKPSSSHSASIE
                     RLQRSINEREIKIQSLFEQEVNNKIKLNEVEKRASVRSQHHTKRVKRAQARIGYNSTG
                     EDILSTLSGKNQSILLRSIQKQHHALEKKCSDLIQEADQINYPLFLEELQKYLNKKKH
                     TLSSQEVDALKSVIKYIKQHLEFEHEVINTQSSLHIKKQSISSQIVKLRELQNKLKTL
                     KNNNPNLTAANEELVSRNLELTAMKEHHADLRHRLGTPALLLFGLTFLFSIPLILTIS
                     GVIPFFIAPALLYILVSAPPTILLLSTLGVGIAAIVFSFKMHSNESAIKSNLQTIETN
                     SNQMSRNSQNLKSLETLTIPTLDMQIKKDENLRDQLMLSLQKQQRQAAQAFQKAKEVE
                     CLSYATSSLLNSGDTQSSDPSLSTHSEESDEGLNDSNDSLQALEEETVNGIA"
     gene            9668..11308
                     /gene="ppeB"
                     /locus_tag="U6309_001224"
     CDS             9668..11308
                     /gene="ppeB"
                     /locus_tag="U6309_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PpeB"
                     /protein_id="HEN5504673.1"
                     /translation="MSLATYDVKQELVKLTQNFFSIDHQFSELKNLLTDKKTIPWIHN
                     QIVDQLSQIKKSDEIAMIARLEKSAYEQQMKEDDDEKNRDVEELRQDLLRRNDLTRQL
                     EILRVRKEQYERELLIRNTVPHVHTHPDTPVIHQHPSQPISAPLLNPNNHVHTHLEAT
                     LSSQDIELSIQKINRRISEINKELEILDSRNNAREIRRGDREKRLQARLNYVQKTAGV
                     VNTLSPDNQKKLLSNIEKEKKSLTQQHSSLLLKADQLNYSIFLQQFELSLQSMQRPFQ
                     EADALKAIVKRMKEHLNYKEKAASIQSRLDNTVLTIGENLRRLTRLNTQLSSLQLANP
                     DLTRRNERLEEQNRELLESYNSHIKTRNKLFFPTLVLSGLSLLFSIPLILTLTGIIPY
                     AIAPAVLLTLVIAPPALLLLAGLGVGIATIIYAVKAYFNNSTIESNQETIESNRRQMG
                     ANQKEIYTLENQTIPNLKKELLENEEIKNRLTDELQYIENLAEQALKQANEVEPYVYS
                     AMPFFNPEVVVHQHPSVGNGIVTPSAPPIDPLYPAVRH"
     gene            complement(11395..12651)
                     /locus_tag="U6309_001225"
     CDS             complement(11395..12651)
                     /locus_tag="U6309_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN5504674.1"
                     /translation="MFALVDCNNFYVSCERLFKPALANKPILVLSNNDGCVIARSNEV
                     KALGVRMGEPYFAVKALCKQHKVHVFSSNYTLYADMSRRVMSVLEDNWPAIEIYSIDE
                     AFLDLNTLPQSEHDEFCRKLQKIVLRHTGIPVSIGIGATKTLAKLANHIAKKELRTPV
                     FNIENQYHWLAKIVVGEVWGIGRQWGKKLVQQGIYTAHDLAGMDLQLIKKLFSVVLQR
                     TVLELRGVSCGSLQDFESRKSIISSKSFGSMQTEYSALAQAISSHCARAWEKLRHQKL
                     MACHLSIFIKTNQFRLDLPQYQQTIGFKLINPTDDLRHLIRCAKLCLRKIFRSGFHYQ
                     KVGVYLGDLIEKNCLQLDLFNQVSEKELNQTERLMSVLDEINTKYGRHTLRLAAEGHV
                     KPWAMRAQSRSPRYTTSWSELPVVII"
     gene            complement(12651..13175)
                     /gene="umuD"
                     /locus_tag="U6309_001226"
     CDS             complement(12651..13175)
                     /gene="umuD"
                     /locus_tag="U6309_001226"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HEN5504675.1"
                     /translation="MSHGGARAGAGRPRGQGKYGESTKSVRIPESRIQDVMKYLQGEK
                     EFPAIPLYTSSVRAGFPSPGDDYIESMLDLNEYLIKHPASTFFVRASGESMINAGIYS
                     GDILIVDRGIEATHGKIVIVALNGELTVKRLSRQHGQVKLLTENPDFPPIDITEEYDM
                     VIWGVVTHVIHTVL"
     gene            13341..14564
                     /locus_tag="U6309_001227"
     CDS             13341..14564
                     /locus_tag="U6309_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN5504676.1"
                     /translation="MVKLYKEIINLIDNHQATMVEQLHQFCEINSGTTNLTGLAIMAE
                     TLCSAFKPIADTTELITLPPLSVIDMTGNTVMQSCGDVLFISKRPHLKRRILLSGHMD
                     TVYSASNPFQKLSYLDANQINGPGVADMKGGLIVILHALAAFENTRFAEDMGWDVLIN
                     SDEEIGSPASGSLFNKLAHRYQAALVYEPAMTPNGTLAKNRKGSGKLTLVATGRAAHA
                     GRAFDEGRNAICYLAEAITAVHALNGQKNGVTINVGKIAGGEALNVVPDKAVAQLDIR
                     ISLPEDEIWVRNELNKIIKQLERPDYSLNVHGTFGRPVKRICAGTERLFHRIQKIGEA
                     LGLTIDWKDSGGCCDGNNLAHYGLPVLDTLGVRGGNIHSSDEYILLDSLSERAALSAL
                     LLIDLAQGGLEDLIK"
     gene            14561..15604
                     /locus_tag="U6309_001228"
     CDS             14561..15604
                     /locus_tag="U6309_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEN5504677.1"
                     /translation="MMLFRSARDSDLDAIHQLAEISGVGMTTLPKDKELLKKRLEWSC
                     ESYQRNVTNPGCEYYFFVLEEQKNNTIVGTSAIEARTGYEIPFYSYKLSKRTRVCHTL
                     NIRSDYEVLSLVNDNQGRTEICTLFLHPDYRKNNNGLLLSKGRFLFMAQYPHLFAPIV
                     IAEMRGISDDKGNSPFWDNVGANFFHMPFVEADRLTLATNKQFIADLMPRNPIYVKLL
                     SPEAQAVIGKPHQSTIPAMNILLKEGFRYNNYVDIFDAGPTIESPLLEIKTISLSRIM
                     VIKNISDEASSSNRYLLANTQMDFRATISHAVFNERENTCIISKETAKLLDVKQGDSL
                     RISPIRTEDTPPL"
     gene            15618..17105
                     /gene="astD"
                     /locus_tag="U6309_001229"
     CDS             15618..17105
                     /gene="astD"
                     /locus_tag="U6309_001229"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HEN5504678.1"
                     /translation="MSKLQIIQSKGQYINGEWIKGNGLILESTNPASGTLLWQGNNAT
                     DEEIANACYVAHRALKSWANTSFEERARYTKAFVEQVEKNREQLARLISLETGKPLWE
                     SQTEVSSVIGKVNLSIQAYQERTWPKQTETAEANACLRFKPHGVVVVLGAFNFPAHLS
                     NGHIVPALLAGNTVLYKPSEHTPAVAELIIQCWHDSGLPPGVINCLQGNANCGNTLLS
                     QDIQGVYFTGSYATGLRIHQQFCNRPEVILALEMGGNNPLVIDEVKDIDAAVYHTILS
                     TMITAGQRCTCARRIIIPDSQTGDLFLERFAKACKLMRIGSFDSQPEPFIGPVISHVQ
                     ALKHLHAQKQLIEMGGEIILPMSLLLEYTGLVSPGIIDMTRAKNPPDEEIFAPFAQIY
                     RYNHFDEAIQLANQTRYGLSAGLLSDNKDHYQQFYQNIRAGLINWNRPTTGAASSLPF
                     GGVGCSGNHRPSAYFAADYCAYPVASMEQPLLTTPVQRLPGLVLE"
     gene            17111..18457
                     /gene="astB"
                     /locus_tag="U6309_001230"
     CDS             17111..18457
                     /gene="astB"
                     /locus_tag="U6309_001230"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HEN5504679.1"
                     /translation="MNVYELNMDGLVGQTHHYAGLSSGNIASTNNALSISNPQAAARQ
                     GLEKMRQLYNMGLKQGLLPPHQRPNLNLLYQLGFKGTPSEQINKAYKTAPELLSACYS
                     ASSMWTANAATVSASVDTEDNKVHFTAANLISNLHRHQEADFSKKLLEFIFSNSDYFN
                     HHPLLPKSMGTSDEGAANHNRLCQSHAHSGINLFVYGKKVLGNHQFEQSPIKYPARQT
                     KEASEAIARNHLLNPERVIFACQNPLAIDQGVFHNDVISVANEHVFLVHEEAFYNQAY
                     VLDQLREKADFPLVIIQISKEQISVSEAVDTYLFNSQLITLPDQKNMILIAPAECQAN
                     LKVKTCIDGLVADPQNPINSVYYLDLKQSMRNGGGPACLRLRVPLNDYELKAMHQGIL
                     IDNDLLDILDKWVLKYYRTELKIPDLADPQLLYECLDALDELTQILKLGSIYPFQS"
     gene            18469..>19115
                     /locus_tag="U6309_001231"
     CDS             18469..>19115
                     /locus_tag="U6309_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504680.1"
                     /translation="MWKTKFGKCIYISPSGYKVYQNLLYRWLTLGSEAWQTVINRYIP
                     RRPGLHYLQMLTLMARKFPNDCCLLGLGGAGIAHLLGAQKTIRSIVAVESSEEVIQIA
                     KKFFMTDLIPHFTITHQNARDFVRENKKTFQHMLLDLYDATHFPDECHSMDFFIHCKK
                     QLTDDGFLAVNLANSQEQWALVQLIKKQFPSNIIIPIKKCANIVVIASKNDNKDL"
BASE COUNT         6067 a         3984 c         3445 g         5619 t
ORIGIN      
        1 gctgtatggc ggcttgttga agacgcaaaa gcaaatatta ttgagcctga acaagaacaa
       61 gtgcctgatt tacgtacgag tggtccgagt tagctaaagc cgcttgtgct cgatcgaggc
      121 ggtgaagcaa catccttgca acttctttcg cggatgcgtc ccaacaggtg tcaataaacc
      181 aggttaataa gttatgcttt gtccaatcat ttgctgttcg cgtccaccag gagatccatt
      241 gatgcaagta actcatggct caaagggaag aaaccccaga ggcaaccatt tgctttactt
      301 gttcacgtgc attgcgaagc gttcaaggat gcggaacgac acgttgttgc acaagctcgt
      361 tgtgttcagt aattgacaca tctgtcttac cctccccctc attgtggtgg aatttaacat
      421 ggaattagga ggaagatagt ttaagtgaat aactaatttt ttagcctttt ctttgacttt
      481 atctgctcga tgataagagg cttggtcaag tattaaatga atgattcctt ttgaaccatg
      541 gtttgctctt atcagactgc tcctgctgtt ctctattcgc tttatgtgga tgacctttag
      601 gtttcttgta gctaaagcca ttacgatgca tccatttatt catccctgga acactataga
      661 taatagacca tttcttctct acataggcga taatataacg caataaaaca gcttttatac
      721 gatcacgctc cttaccgtta gagcaacaac tattccttgc ttccagccct tcttttcttc
      781 tggtgataag tgtattcttt tcatgcggca tagcatgatc ttctcagatc aaaaaagcaa
      841 gcattttcat agatcagcgg tatagatcag ccctcataca agaacaacca aatattgccg
      901 ttccagacag gggcagatct cctgtaataa attcacttaa ggttttatta ttctcttaag
      961 tgaatttcta atactgctaa ccttcttccg ggatagacat taaactggca ttacctcctg
     1021 cagcagttgt atcaactgta taggtacgct catggcaaag tcgaatcaaa tagttagggc
     1081 ctccagcctt gggtcctgtg ccagataacc cttcaccacc aaaaggctgt agacctacta
     1141 ctgcgccaat catattacga tttacataac agttccctgc gtgaaccctt tgcctaatgt
     1201 agtcaactgt ttcattaatt cggctgtgta ttcccagtgt taaaccatag ccagtttgat
     1261 tgatttgatt aattacctta tccagatctt ttctcttaaa ctgaatcaca tgcaaaattg
     1321 gcccaaatac ttctttttcc aaagcgctga tactatcaat tgcaatagct gttggcggca
     1381 tgaaatatcc tgataaagct tcatcgtcaa cagtgcattg atataatatt tcatgatgtt
     1441 ttctcatgtt ttcgacatga tttttcaata tagataatgc ttccttgtct ataacagggc
     1501 caacatcggt tgataaccat tgaggatccc ctacgactag ctcagccatg gcacctttta
     1561 atagctctac agttcttgga tacacttcct cttgcacata caatacccta agtgccgagc
     1621 atcgctgccc cgcgctgcca aaggcagaag tgacagcatc aacaaccact tgttccaata
     1681 atgctgaaga atcgacaatc attgcatttt gaccacctgt ttcagcaatt agaggaatga
     1741 tttcaccacc tcgagtagct agcgtccgat taatcaaatt cgccgtgtca gtcgagcctg
     1801 taaataatac tgctttaatt cgtttatctg cgaccaaagc agcgccaata gtctctccag
     1861 cgcctggaat taattgaatt actccttcag gaataccggc ctgatgcatt aacttcacag
     1921 cataggctgc aattagtggc gtttgttctg ccggtttcgc aataacacaa ttgcctgtaa
     1981 ccagcccggc tacgacttgt cccgtaaata tagccaatgg aaaattccag ggactaatac
     2041 acaatattgt cccgcgagga tgcaaactca attcatttag ttccccagtg tatccattaa
     2101 agcgttgagg gctactcata agctcttgag ctttcttggc atagtatcga caaaaatcaa
     2161 tagcctctct tacttcagca atgccatcac tccaggtttt accagcttcc cgacaagtca
     2221 ataccatgag ctctgccata ttggcctgta ataaatctgc aaatcgattt agacaagaag
     2281 cgcgttcttc tactggtttc ttgctccaca attcaaaagc caattttgcc tgatttaatg
     2341 ccacctctac atcatcaagt gtggcttgct gcactgagcc aatagcatat gccggctgct
     2401 gtggggacat aactgtttgc agtaagtccc tggataattt tcgaccagca atcataggac
     2461 ttgcctgcca ttcttttgat tcaatttttg ccagttcctg ctgcaataat gcgcgttcaa
     2521 gtcgatttgt aaagtcaaag cctttagaat tttttcgtac gggtaaaaag atatcctctg
     2581 gcaatggtat atttttattt attttatcaa gtaacgatct tgatttagct accgggtcct
     2641 ccactaactc gctaataggt gccttatcat caactatgcg attgacaaaa gatgagtttg
     2701 ctccattttc cagtaatcgt cgaactaaat acggtaataa atcctcatgg ctaccaactg
     2761 gtgcataaat acggcatgga ataccataac aatttgctgg aacaatctgt tcataaagtt
     2821 cattacccat accatgcaga cattgaaatt caaaatctct ataacctcct accaaattaa
     2881 gaatcattgc tacagaataa gcattatgtg tggcaaattg tggataaatg gcatcggtca
     2941 tagtcaggat tttttttgcg caagcctgaa atgacacgtc agtaaatact ttacgggtaa
     3001 aaaccgggta ttcagaaaag ccttgcattt gcgttttttt gatttcacta tcccaatagg
     3061 ctcctttaat caatctgacc ataatacgcc gctgcttact tcttgctaaa gcagcaaccc
     3121 aatccaagac ataaaaagct cttttttgat aggattgaac ggccaaaccg aacccattcc
     3181 atccctgtaa actctcatca gtaaacactt tctctatcac atcaagcgaa agatctaaac
     3241 gctctgactc ctcggcgtca attgtcaaag caataccata atcttttgct aaacgagaca
     3301 atgctaataa tttggggggt aattctgcca tgacacgctc atactgaaac tcgctatatc
     3361 gtggatgtaa ggcagaaagt ttgatggaaa taccagggcg tctatataca tctgaatgtt
     3421 tatcagcttt ctctccaatt gaaatgattg cttccttgta ggcttcaaaa tatcttgcag
     3481 catcagcaga tgttaaggca gcttcaccaa gcatatcgta agaatatctg tatcccctgt
     3541 cttccttttt tttcgctcga gctaacgctt cattaatggt acgtcccatc acaaattgtt
     3601 tgctcataat tcgcatggct ttatccactg cttttctaac aacagcctca ctacttcgat
     3661 taaccaattt tagtaaagct ttggttaatg tattttcagc tttttcagga gttaaaactt
     3721 taccagttag cattaaagcc caagtagtag catttacaaa aaatgattca ctctgtccac
     3781 gatgcgcccc ccaatcaccg ccagctaatt tatctttaat caagttatct atggtggcat
     3841 tatcaggaac gcgtaataaa gcttctgcca gacacattaa ggcaattccc tcatcacttg
     3901 ataaggcata ctccgttaaa aaagaatcta ttcctgtgct tttctttctc tcactacgaa
     3961 ctgattgaac caattttgta gcactggttt taattgccat catttgctga ggatcaagtg
     4021 ctgcctgttc actaagctct gtaattaaag aaagctcatc catgcgataa gctttattaa
     4081 tagcagctct taatccttcc ggtaaatgaa tggactgctt ttccagcata atgttctcca
     4141 agactaaagt ggtaattgaa aaaaatagtt attaaatttt taccatataa gacaaaaatt
     4201 aaattcatta ttataacaat gaaaaaatcc ggttatgata tcttacactg gcttatttgc
     4261 catgcccctc tctacgacaa ggcaatattt cagatataaa tcatagtatc gagcaataaa
     4321 ttgggctata taaaatcaat gcatcaaccc cctttttgtg gcatattata tgacgacaat
     4381 cctgttcgat aaagtaaaaa catgcttttt aacccagaaa aatagaacta aatttaacca
     4441 acaagatcta attataatca ataagatgtt tttttggtta tacatataga gcaattagtg
     4501 aactcattga aaacattttg ctccattctc tttttcagcc tgtaattaac ctcatgatat
     4561 gattaatatt tttacctaat gggcaactta aacccttata aatttacaat taacttattt
     4621 gttaatataa tcaccatctt ttgtaataac caataatggt gatttaacaa gaagaaagga
     4681 gcagtatgaa tacattattt actctaatct caactgtact actaacaagt aatatcacat
     4741 ccagcactcc attatttgcc aagcctggaa cagacattaa cacccaggta caacatctta
     4801 gccacaaggc ccctcaatta aacaagaaag tattaaagct tgctttaacg gcctataaaa
     4861 atgcgagtaa gagaggagca gttaaaaagc ctgtcctcac agtaattgat tattccttgc
     4921 cttcaaataa gcaacgtatg tgggtattcg atcttcgtaa tgaacgtcta ttatacaata
     4981 cgtacgtagc ccatggtaaa aattcgggag ttaatgttgc acggagtttt tctaacagag
     5041 agtccagcaa acaatccagc ttaggtacct atatcacaaa aaacacctat atcggacaca
     5101 aaggctactc cttgaacctc caaggcctgg atagaggctt taatgataat gcatacaata
     5161 gacgtgttgt gatacatggc gcgtggtatg ttgaacctga atttattaaa aaggcaggtc
     5221 gcgctggttt atcctggggc tgcccagcta tagcacaaac cttggcaaaa ccggtgataa
     5281 ataccattaa aaatggttcg gttgtttttg cttattaccc tgataaaaag tttttatctc
     5341 actcaggtta tcttgtagca taaaaaagta agccagggac tcagccctgg cttttcgcag
     5401 tcatccgtat aagaacttgc gttcttattg tcatcctgac aaatcagatc catctaattg
     5461 caaatccatt tgcacgactc atatccatat aagtcttgat taaaatttag caattgaata
     5521 ggggaagcgc aaggtgttaa atcacacctt cactcaatac tattttcaga tcagaaatag
     5581 tcttatatat ttattttatt aataatcaat aacttaccta cttatgtaag aattattgcc
     5641 aggtaaacag aataatttct tctatggaat tgagaaattt cttacatcaa ttacagctta
     5701 aattacgtca ttttatcttt tagagcaata tgctgcttga ttatgtcata agatatggta
     5761 gtttatttac gatgaaatgt agcggggtat gattatgaac caacaactaa atgcaaccaa
     5821 gaaagataag cttcaagtaa tcgattggct tattgaaaat tttcctaatg cattttttaa
     5881 aaaaggaaac caggtcaaac cgctcaaaat tggaatattt gatgatctaa ttgattttta
     5941 tgagcgactg gatactccgc cttttagtaa aaaatcgtta agagaagcat taagctatta
     6001 cagtgcttct ccagcttact taagctgtca aaaacctgat acagcacgtg tagatattta
     6061 tggtaatgaa gtcgatgtag ttacgccaga gcaggccaag tatgcttatc agcgttatca
     6121 ggagcgctat ggcaataaaa aaagccaaga gttaaaatag cgcggaaaag ccacatttgg
     6181 atttgtttta ttgtaggacc ataatgtaat taatgcttac atcagaaacc agagtggtct
     6241 tcctaaggaa aggattatag cataggcctt tcatatcaat gagatttaaa tcgtaaaggc
     6301 gtgccatttt aactaactca ctgggtttaa tgaatttatc ataatcgtgt gtttgcctgg
     6361 gtaacaaatt aagcacatac tccgcagcaa taatagcgcc gagataagct tttaacgttc
     6421 tgctaatagt tgataaaaat aatagcccat ttggttttaa taaacgcctg caatgttgta
     6481 aaaccaactc agggttttgt acgtgttcca ataactccat acaagttatt acatcaaaac
     6541 cttgctcctc atactcttca ataggattgc aaaaataatc aatctgcaac tggtttttat
     6601 gagcatgttc tttggcaatt tgaatagctt ctggttctgc atctaatcca ctcacataag
     6661 ctcctgcctt ggccatagat tcacagagta tcccgccacc gcaaccgaca tccaacaccc
     6721 tcagcccctt tagattaatg tgctctgaaa taaactcaaa acgagcgcca ttgatatcat
     6781 gcaaagttct gagaggacca ttagtatccc accaatcatt tgcaagttgg gcaaatttat
     6841 gtacttcttc aatagcaata gtcgattcaa tttgattcat aagacaataa atccattggt
     6901 ttaaagaatt ctataacacg ttgattagta ataaagctta cattatatgg gtgttctgaa
     6961 accagtccta tcaataattt atttgcgggt attttctcag caattttacc aaataagatt
     7021 aattgcacat caggcttcct gatagccaat tgctcaaata aattttgcat aaatggcttc
     7081 caatgacgcg cgtgatacgg aaccttacct ttactgtata tcagggaagc attcaataat
     7141 aaaattccct ttttcatcat tcctttaaaa aaatcttccg cagtttgtac cagacatgat
     7201 ttatttatct tggcgattgc ttcttgcgag gtatttccat gcaaatcacc ccgagcgatc
     7261 aataacgttt taatccagtt gcgtagtgag gttgctcgat taacttcctt acttagccct
     7321 gtggagctcc acaataaacc tacagagtta tcccaaaaag catacccatt agcagaaatt
     7381 ttgcgtggat atggggactc gcctaaaaga atatattgtg tctggctcaa aggcaggcta
     7441 aaagcagaaa acagttgatt tagcccaggt aaccaatggc tatcctcttg tatttgtttt
     7501 aagtaatcac tatccattgt attcaatgct ttcatcagaa tctctttcca ttcctcatgg
     7561 catccaacca ataaattctc atacatctta atttttaccc ttccgtcatg ataattaaat
     7621 tttcaaattg aggtattttg ataattacct atgtttttaa accaggtttt cagccacttt
     7681 aacactattc ttagaccatg agaaattgtc ttattttaac atctggaaat aaaccccacg
     7741 ttggccaata gcttatctgt tgattgcagc gattttcttg ccatatcaat cgtggtgtgt
     7801 tgcctgaata ttcaaattac actaacatca tttaaaatga taatctggag tgattatgtt
     7861 tttggccaac tgcaatatcg aggaattagt tacagaacat ataaaacaat ttctagcgga
     7921 tgaagagttg tctttctctg gcttaaaaga tttaattctg agtaaagccc caattccctg
     7981 gattcactcc tctgtaacag ccactctgct taaatccaga gactccgata aaacagaagt
     8041 aaaaaaaaac cttgaacaac aatcttataa agctcaactg gctgaagaca agattcaaaa
     8101 agagcaggat gatgctgaag cattaaaaga taaaaaatta aaggaagttt taactcgtga
     8161 attaaaccac atcccaactc aaatcagtga acagcaaacg gaactcagat tacttcatta
     8221 taaactagag cgactctttg aaagccaagc taaagtcgat gtgattcaac attctgattc
     8281 tcctatgaaa aagataaagc cgtcctccag tcactctgcg tcgattgagc gcctgcaaag
     8341 atctattaat gagcgtgaga ttaaaattca atcacttttt gaacaggaag ttaacaacaa
     8401 gataaaatta aacgaagttg aaaagcgtgc cagtgtacgc tcacaacatc acactaaaag
     8461 ggttaaacgc gctcaggcaa gaattggata caatagtact ggagaggata ttctcagtac
     8521 attatcaggg aaaaatcaat ccattttgtt gcgcagtatt caaaaacaac atcatgctct
     8581 tgaaaaaaaa tgcagtgatc tcattcagga agctgatcag attaattacc cattatttct
     8641 tgaggaactt caaaagtatt taaataaaaa aaagcatact ttatcttctc aagaggtcga
     8701 tgcattaaag tctgttataa aatatataaa gcaacacttg gaatttgagc atgaggtaat
     8761 caatacacaa tccagtctcc atattaaaaa gcaatccatc agttcccaga ttgtaaaatt
     8821 acgagaatta caaaataaac taaaaacctt aaaaaacaat aaccccaatt tgacagcggc
     8881 aaatgaagaa ttagtttcac gtaatttgga attaacggcc atgaaagagc atcatgctga
     8941 cctacgtcat cgcttgggca cacccgcatt acttttattt ggattaactt ttctattttc
     9001 aattcctctt attttaacca taagcggagt aattcctttt ttcattgcgc ctgcgttgct
     9061 ttatattttg gtctcagcgc ctccaaccat cttgctgcta tcaactcttg gtgttggcat
     9121 agcagctatc gttttcagct tcaaaatgca ttccaatgaa tcagctatta aatccaattt
     9181 gcaaacgatt gaaacaaact ctaatcaaat gagccgaaat tcccaaaatt taaaatcatt
     9241 ggaaacgctt accatcccca ctctggatat gcaaatcaaa aaagatgaaa atttaagaga
     9301 tcaattaatg ctttctttac aaaaacagca acgtcaagct gcccaggcat tccagaaagc
     9361 aaaagaggta gagtgcctat cctatgctac ctcttcactt ttaaattccg gcgataccca
     9421 atccagtgat ccatcattat ccacgcattc agaagagagc gacgaagggc taaatgactc
     9481 gaatgactct ctgcaagcgc tggaagaaga aacagttaat gggatagcgt aaattgcctt
     9541 tctgtcaata aaatttcaat ggagcaagac tgagtattaa ccccctgtaa taaaactgct
     9601 agtcccaata ttgataaatt agtctttatg atctaaaatg caacaacttg attattggga
     9661 gtaatctatg tcacttgcta cttatgatgt aaaacaggaa ctcgtcaaac ttacccagaa
     9721 tttcttctct attgatcatc aattttctga attaaaaaac ttattaaccg ataaaaaaac
     9781 cattccatgg attcataatc aaatagttga tcagttatct caaattaaaa aatcagacga
     9841 aatagctatg attgcgcggt tagaaaaaag cgcttacgaa caacaaatga aagaagatga
     9901 cgatgaaaaa aatcgggatg ttgaagagtt acgccaagat cttctccgaa ggaatgattt
     9961 gacaagacaa ctcgagatct tgcgagtccg caaagagcaa tatgaaagag agttgctcat
    10021 ccgcaataca gtaccccatg tccatactca tccggacact ccggtaattc atcaacatcc
    10081 ttctcaacca atatctgcac ccttgctaaa ccctaataat catgtacaca ctcatcttga
    10141 agccacttta agtagccagg atattgagct gagtattcag aagattaaca gaagaatttc
    10201 agagataaat aaagagttgg aaattctcga ctccagaaat aatgcaagag aaattcgcag
    10261 aggcgataga gaaaaacgcc ttcaagcacg ccttaattat gttcaaaaaa cagcaggagt
    10321 tgtcaacacc ctatctcctg acaatcagaa aaaactgtta tccaatattg agaaagaaaa
    10381 aaaatcactc acacaacaac attcctcctt gcttctgaaa gcagatcaat taaattactc
    10441 tatttttctt cagcaatttg agctctcttt acaatctatg caacgtcctt ttcaagaagc
    10501 agacgcattg aaagctattg taaaaagaat gaaagagcat ttgaattaca aagaaaaagc
    10561 ggcatcgatt caatcccgat tagataatac agtgcttact attggtgaaa atttaagaag
    10621 attaacgcga cttaatactc aattgagttc cttgcagctt gctaatcctg atttaacaag
    10681 gaggaatgag cgcctggaag aacaaaacag agagctcctt gaatcgtata atagccatat
    10741 aaaaacacga aataaactgt ttttcccgac tctcgtttta tctggattgt ccttgctctt
    10801 ttctattcca ttgatattaa ctctaactgg aatcatcccc tatgctattg caccagctgt
    10861 attgttaact ttggttattg ctccccctgc attgttattg cttgctggac ttggtgtcgg
    10921 tattgccaca atcatttatg ctgtgaaagc ttacttcaat aattcaacga ttgaatcaaa
    10981 ccaggaaaca atagagagta ataggcggca aatgggagca aaccaaaagg aaatttacac
    11041 tctggaaaat caaacaattc ccaatcttaa aaaagaattg ttagagaacg aagaaattaa
    11101 aaatcgctta actgatgaat tacagtatat cgaaaacctg gccgagcaag cattaaaaca
    11161 agcaaacgaa gtagagccat atgtctactc agctatgcct ttctttaacc ccgaggttgt
    11221 tgttcatcaa catccttctg taggtaatgg tatagtaact cctagcgcac ccccgatcga
    11281 tcccctctat ccagccgtcc ggcattaatt tccagcctgg gttattaaca caggctgtca
    11341 tccgggatga cttaagttga tttcccggat gacattatag ctatcagttt ctctttaaat
    11401 aattaccaca ggcaattcag accaggaagt tgtatagcga ggagaacggg actgcgcccg
    11461 catagcccat ggcttcacat gcccttctgc tgccaaacgc aaggtatggc ggccatattt
    11521 cgtgttgatt tcatctaata ccgacatcaa tcgctctgtc tgattcaatt ctttctctga
    11581 aacctggtta aacaaatcta actgcaaaca gtttttttca attaaatctc ctaaataaac
    11641 gcctaccttt tggtaatgga atccagagcg aaatatttta cgcaggcaca atttggcaca
    11701 acgaatcaaa tgtcgcagat catcagttgg attaatcagc ttaaacccta tagtttgctg
    11761 atattgtggc aaatccaaac gaaattgatt ggttttgata aatatggaaa gatggcatgc
    11821 catcaatttt tgatgacgta acttttccca agctctggca caatggctgc tgatggcctg
    11881 agcaagagca gaatactctg tttgcataga accaaaggat ttggaagata ttatgctttt
    11941 tcttgactca aaatcctgta aactgccaca tgaaacccca cgcaactcaa gcactgttcg
    12001 ttgcaataca acactaaata attttttaat taattgcaaa tccataccag ccaagtcatg
    12061 agctgtataa atcccctgtt gtactaactt tttaccccat tgccgtccta tcccccatac
    12121 ctcgccaaca actattttag ctagccaatg atattgattt tcaatgttaa atacaggggt
    12181 tcttaattcc tttttcgcaa tatggtttgc gagtttagcc agggttttgg ttgcccctat
    12241 gcctatggat acgggaatac cggtatggcg caaaacaatt ttttgtaact tgcggcaaaa
    12301 ctcatcatgt tcagattggg gcaaagtatt caaatcaaga aaagcttcat caatggagta
    12361 aatctctatt gctggccaat tgtcttcaag aactgacata acccggcgtg acatatcagc
    12421 atacaatgtg taattggatg agaacacatg cactttatgc tgtttacata atgctttaac
    12481 tgcaaaataa ggctctccca ttcgaacacc caaagctttt acttcattag atcgcgcaat
    12541 cacacaacca tcattattag acaagaccaa aatgggttta ttcgctaagg ctggtttaaa
    12601 taaacgctca cacgagacat aaaaattatt acaatcgacc agcgcaaaca tcacaatacc
    12661 gtatgaataa catgagtgac gactccccaa atcaccatat cgtattcctc tgtaatatct
    12721 atgggaggaa aatcagggtt ttcagtcagc aacttaactt gcccatgttg acgggataaa
    12781 cgttttactg tcaattcgcc attcagggca acgattacaa tttttccatg agtagcctct
    12841 atgccacgat caacgattaa aatatcaccg gagtaaatac cggcatttat catggattct
    12901 ccagaagctc gtacaaaaaa tgtggatgca ggatgcttga tcaggtactc attcaaatcc
    12961 agcatggatt caatatagtc atcacccgga gaaggaaaac cagctcttac agaactggta
    13021 tataaaggaa tcgctgggaa ctccttttct ccttgaagat atttcatcac atcctgtata
    13081 cgagattcag gtatacggac tgatttggtt gattctccat atttaccttg cccacgaggg
    13141 cgcccagcgc ctgctcgtgc accaccatga gacatatact cccctaaact tgaatttaga
    13201 atcaaaattc aagtttaatt ttaaagtagc ccattgtaaa gttccgacaa taatgagagt
    13261 ttcatatgac gactaaactg aattgacgcc aatactcaaa ttacgttatt gttggggatt
    13321 tagtgtaaga ctaaagagcc atggttaaat tatataagga aataatcaat ttaatagata
    13381 atcaccaggc aactatggtg gagcaactcc atcaattttg tgaaatcaat tctggaacaa
    13441 ctaacttgac aggattggcc atcatggctg aaacgttgtg ttcagctttc aaaccgatcg
    13501 ctgataccac agaattaata acattaccgc ctttgtctgt gattgacatg actggaaaca
    13561 cagttatgca atcctgtggg gatgtattgt tcattagcaa acgtcctcat ttaaaacgcc
    13621 gaattctgct aagtgggcat atggatacgg tttattccgc cagcaaccct tttcaaaaat
    13681 taagttacct ggatgctaat cagatcaatg gcccgggggt tgctgatatg aaaggcgggc
    13741 taatcgtcat cttgcacgca ttagcggcct ttgaaaacac cagatttgcg gaagatatgg
    13801 gttgggatgt gttaattaat tcagatgaag aaattggttc acctgcatca ggttccttat
    13861 tcaataaatt ggcacatcgc tatcaggccg ctttggttta tgaaccagca atgacaccta
    13921 atggtacttt ggcaaaaaac cgcaaaggca gtggaaaact aacactggta gcaacaggaa
    13981 gagctgccca tgctggccgg gcatttgatg aaggaaggaa cgccatatgc tatcttgctg
    14041 aagcaattac ggctgttcat gccttaaatg ggcaaaaaaa tggagtaaca atcaatgtag
    14101 gcaaaatagc tggcggagaa gcgctgaatg ttgttcctga taaggcagta gctcagttgg
    14161 atatccgaat tagtctgcca gaagatgaaa tttgggtaag aaatgaatta aataaaatca
    14221 tcaaacaatt agagcgacct gattattccc taaatgtgca tggcaccttt ggacggcctg
    14281 tgaagcgtat ctgcgcaggg acagaacgac tttttcatcg gattcaaaaa attggagagg
    14341 cgcttggctt aactattgac tggaaagaca gtggaggttg ttgcgacggt aataatttgg
    14401 ctcactatgg cttgcctgtt ctggatactc taggtgttcg gggaggcaat atacacagtt
    14461 cagatgaata tatattgctt gatagcttat cagagcgcgc cgcacttagc gcgcttcttc
    14521 tgatagattt ggcacagggt ggtttagagg atctaataaa atgatgttat ttcgtagcgc
    14581 tcgcgactct gacttggatg cgatccatca actggcagaa atcagtggtg ttgggatgac
    14641 aacgctccct aaagataaag aattattaaa aaaacgcttg gaatggtctt gcgaatccta
    14701 tcaaagaaat gtcactaacc ctggctgtga atattatttt tttgtcctgg aagaacaaaa
    14761 aaataataca attgtcggta cctccgctat agaagcaaga acaggttatg aaataccttt
    14821 ttattcttat aaactctcta aaagaaccag agtctgtcac acactcaaca tacgcagtga
    14881 ttatgaagta ttaagtttag tcaatgacaa tcaaggtcgc acagaaatat gtactctgtt
    14941 tcttcatcct gattacagaa aaaataacaa tggcctattg ctttcgaaag gacgcttttt
    15001 atttatggca caatatcctc atctcttcgc cccgattgtc atcgccgaaa tgcgtgggat
    15061 ctctgacgat aaaggcaact cgccattctg ggataatgtt ggagctaatt tttttcatat
    15121 gccttttgta gaagcggatc gtctgacact agcgacgaac aaacaattta tcgccgattt
    15181 aatgccccgc aatcctatct atgtcaaact cctttcgcca gaggcgcaag cagtcattgg
    15241 caaaccacat caatcgacta tccctgccat gaatattctc ttgaaagaag gctttcgtta
    15301 taacaactac gttgatattt ttgatgccgg gcctactatc gaatcaccat tattagaaat
    15361 taaaaccatt tctttaagcc ggatcatggt tattaaaaac atcagtgatg aagcgagcag
    15421 tagcaatcgc tatttactcg ccaataccca aatggatttt cgagcaacaa ttagccacgc
    15481 tgtttttaat gaacgagaaa acacctgcat tattagcaag gaaacagcaa aattgcttga
    15541 tgtaaaacaa ggagacagtc tacgtatatc acccatacgt acagaagata ctccaccatt
    15601 gtaaccaagg aataatgatg tctaaattac aaatcataca gagtaaaggc caatatatta
    15661 atggagaatg gataaaggga aatggtctca ttctggagtc tacaaacccc gcctccggaa
    15721 ctttgctttg gcaagggaat aatgctacag atgaagaaat tgcaaacgct tgttacgttg
    15781 cgcatcgcgc actgaaatcc tgggctaata ccagttttga ggaaagggct cgttacacta
    15841 aggcttttgt ggaacaagta gaaaaaaatc gtgagcaact ggcacgactt atttcattag
    15901 aaacaggaaa gccattatgg gaatctcaaa ctgaagtcag ctcagtaatt ggcaaagtaa
    15961 atttgtcaat tcaggcttat caagaaagaa cctggccaaa acaaactgaa actgcagaag
    16021 ctaatgcctg cctccgtttt aaaccgcatg gtgttgttgt agtattaggc gcgtttaatt
    16081 tccctgccca tttaagcaat ggacacattg tccctgccct ccttgcaggg aacacggtat
    16141 tgtataaacc aagtgaacat actccagcag tagcggaatt gatcatccaa tgttggcacg
    16201 atagcggctt acctcccgga gttatcaatt gtctacaggg taacgcaaat tgtggaaata
    16261 ccctattgtc tcaagatatt caaggcgtct attttaccgg gagttatgca acagggctgc
    16321 gtatacacca acaattctgt aatagacccg aagttatttt agctttggaa atgggcggaa
    16381 ataacccttt agtcatcgat gaagtgaaag acattgatgc agctgtatac catacaatcc
    16441 tttcaactat gatcactgca ggacaacgtt gcacatgtgc gagacgaatt ataattcctg
    16501 actcccagac aggcgatcta tttttagaac gctttgccaa ggcttgtaaa ttaatgcgaa
    16561 ttggatcctt tgatagtcag ccagagcctt ttattgggcc tgttattagc catgttcaag
    16621 cattaaaaca tctacacgct cagaagcaac taatcgaaat ggggggtgaa atcattctgc
    16681 cgatgtcgct tcttcttgag tacacgggac ttgtttctcc tggcattatt gatatgactc
    16741 gcgccaagaa cccgccagat gaagagattt ttgcgccatt tgcacaaatt taccgttata
    16801 atcactttga tgaggcaatt caattagcga atcagacacg ttatggtttg tcagcaggct
    16861 tactcagcga taataaagac cattatcagc agttttatca aaatattcgt gccggactaa
    16921 tcaattggaa cagaccaacc acgggtgctg ccagtagcct tccatttggt ggtgtcggat
    16981 gcagtggaaa tcatagacca agcgcctatt ttgctgctga ttattgtgct tatcctgtag
    17041 caagcatgga gcaacctctg ttaacaactc cagtgcaacg attgccaggt ctggtattgg
    17101 agtaattatt atgaatgttt atgaattgaa tatggatggg cttgttggtc aaactcatca
    17161 ttacgcaggg ctctcctcgg gtaatatagc ttctacaaat aatgctttat ctatttccaa
    17221 ccctcaagct gcagcaaggc aaggtttgga aaaaatgcgg caactttata acatggggtt
    17281 aaagcaaggc ctattgcccc cacatcaacg acctaatttg aatttactgt atcaactggg
    17341 atttaaaggc actcccagtg agcagataaa caaagcctat aaaacagcgc ccgaattgct
    17401 tagtgcctgc tactctgcct caagcatgtg gacagctaat gcagcaactg tttctgcaag
    17461 cgtggatact gaagacaaca aagtacattt cactgctgcc aatttaatca gcaatttgca
    17521 tcgtcaccag gaagctgatt tttcaaaaaa attattggaa tttatctttt ccaattctga
    17581 ttattttaat catcaccctc tgttacccaa atcaatgggc acaagcgatg agggagcagc
    17641 taatcataat cgcttatgtc aaagtcatgc gcactcaggg attaatctct ttgtatacgg
    17701 taaaaaagtg ctggggaatc atcaattcga acagagccca ataaaatacc ctgctcggca
    17761 aactaaagaa gcgtcagaag ccatcgctcg caaccattta ttaaatcctg aacgagtaat
    17821 ctttgcctgt caaaatccat tagcaataga tcaaggtgta tttcataatg atgtcatctc
    17881 agttgctaat gaacatgtat ttctggttca tgaagaagct ttttataatc aggcttatgt
    17941 tttggatcaa ttaagagaaa aagccgattt tcctttagta ataattcaga tctcaaaaga
    18001 acaaatcagc gttagtgaag cagttgatac ctatcttttc aattctcaat tgattaccct
    18061 gcctgatcag aagaatatga ttttaatagc ccctgctgaa tgtcaggcaa accttaaagt
    18121 aaaaacttgt attgatggat tggtcgctga tcctcaaaac cctattaact cagtgtatta
    18181 cctggatctg aaacaaagca tgcgtaatgg cggaggacca gcctgtttac gcttgcgagt
    18241 tcctttgaat gactacgagc ttaaagcaat gcaccaaggt attctaattg ataatgattt
    18301 gttagatatt ttagataaat gggttttaaa atattaccga actgaactga aaatccccga
    18361 tttagctgat ccgcaactat tatatgaatg tctggatgca ttggatgaat taactcagat
    18421 cttgaaatta ggctcaatat atccctttca atcttgagta tccgagtcat gtggaaaaca
    18481 aaattcggca aatgtatcta tatttcaccg tcaggatata aagtctatca aaatttgttg
    18541 taccgttggc ttactttagg tagcgaagca tggcaaactg taattaaccg ttatatacct
    18601 cgcaggcctg gtttacatta tctgcaaatg ctgacactta tggcgcgaaa atttcctaat
    18661 gattgttgtt tattaggact tggaggagca ggaatcgctc acctgctggg tgcacaaaaa
    18721 accatacgct ctatcgttgc cgtggaaagt agtgaagaag tgattcaaat tgccaaaaaa
    18781 tttttcatga cagatttaat tcctcatttc accattactc accaaaacgc ccgagatttt
    18841 gtacgtgaaa acaaaaagac ttttcagcac atgcttcttg atctatatga cgcaacacat
    18901 tttcccgatg agtgtcattc tatggatttc ttcattcatt gtaaaaaaca attaactgac
    18961 gatggatttt tagctgttaa cttagcaaat tcccaagaac aatgggctct ggtccaacta
    19021 atcaagaaac aatttccatc caatatcatt attccaataa aaaaatgtgc caatattgtt
    19081 gtcattgcct ccaaaaatga caacaaagac ttgtt
//
LOCUS       DASLOE010000043        18060 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.043, whole genome shotgun sequence.
ACCESSION   DASLOE010000043 DASLOE010000000
VERSION     DASLOE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18060
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.043"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..826)
                     /locus_tag="U6309_001232"
     CDS             complement(<1..826)
                     /locus_tag="U6309_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504681.1"
                     /translation="MKWQIPAKTFLLGEYAAIAGEPAILLTTSPCFILTLTENAKLEG
                     IHPESPAGLWWQKNQCYPFGLSWQDPYHGQGGLGASSAQFLACYLATCSLQEITPRLN
                     YMLEAYYQSSWSGLGLRPSGYDVIAQSQQGCVYIDKQKNQVNSFSWPFKDLSFLIIHT
                     GNKLATHHHLQEVTLPSQIDYLASLVIKAKEAFDLAHSHHLIQCINLYAQKLTELNLV
                     SQHSLPLIQSIKSYPETLAIKGCGALGADTLLILTDKRDLQSLKDKVQDQNWLILAT"
     gene            complement(823..2121)
                     /locus_tag="U6309_001233"
     CDS             complement(823..2121)
                     /locus_tag="U6309_001233"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEN5504682.1"
                     /translation="MSIAANAGELFRGFSKLSREERFQRLCALGALTHEDITFLKQGG
                     IKDLNLADKLIENVIGYFQLPLGVATNFNIDGRDYVIPLAVEETSIIAALSKSAKWIR
                     QHGEINTWVHGECILGQIQLAKVKDFQRFSDLFNKNRQYFIEIANKDVAANMVKRGGG
                     VTDLQVRHLKREDGQDMAVIHLTMNSCDAMGANIINQVLEYLKQPIEQITGEEVTMCI
                     LSNLNDQKLTTAQVIIRNIDPILGQKLQEASLFAEIDPYRAATHNKGVMNGIDPVLIA
                     TGNDWRAVEAGIHAYAARSGQYKAITRWRYQNEILTGELTAPIIVGTVGGVTSLHPTA
                     KMCLRMMDITSANQLSQVIAAVGLVQNLGALKALCTDGIIQGHMKLHIDNLLLVAGAN
                     ENEMPVLKEKLQEWLNMNKRVSLNNAYDLLAEIRQAPVAV"
     gene            complement(2112..3140)
                     /locus_tag="U6309_001234"
     CDS             complement(2112..3140)
                     /locus_tag="U6309_001234"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEN5504683.1"
                     /translation="MQNEYSQFEQRKRDHIELALMPANQSSELNPFDHFSLVHEALPD
                     LDFKDISIQSIRFKKPVEKPFIISSMTAGHSNALEINYRLMEACSKTKWAMGVGSQRR
                     ELTDKQAAFEWAPLRRDFPMVSLFSNLGIAQLIDTPISAIERLIDTLQAEALIIHCNP
                     LQECIQPEGTTNFQGCWAALEALVKKIASPVIIKETGCGFSKNTLLRLNNIGVAAVDV
                     SGVGGTHWGRIEGHRANKDPIRHRTADTFRNWGIDTLQSIRNAISLNPSFETWGSGGV
                     RNGLDAAKLFALGATTVGFAKPMLEAALDSTGQVLTQMNTIEYELKTAMFCTGSRVLD
                     DLKEKACP"
     gene            3308..4345
                     /locus_tag="U6309_001235"
     CDS             3308..4345
                     /locus_tag="U6309_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2050 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504684.1"
                     /translation="MKSKLEYHFSANADGDESCIEDVDGNEFTPNGSPSHGWTAWTRF
                     FGHQNQSPKYVVKQPRPDKVRSSSDLRDKKLNNEWNRTRYQHELDMWNLVYPDNKAYL
                     FTDNGIRLVLPYLPGKTLFSFTSREEDQLTWCQLNYAVFCEFKRFIELGLKHCDVKYD
                     NFLIERKQDGTFKAYIIDLDSVGKLKPNDQDYDWNIVLASLFDDTGLKSPDVCPPGYI
                     ALKSIDVPAENENEARSKLDKIKKELSQKKPTLVRIKNCQQNKLDYYFVYGCLYNGQW
                     GFTKLDEKIISSVNLSNFSGALVDCRAASVIGNHIKLKQGHISYRLDLYINCLNEKIA
                     ELTLLKAHLKS"
     gene            complement(4497..4796)
                     /locus_tag="U6309_001236"
     CDS             complement(4497..4796)
                     /locus_tag="U6309_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504685.1"
                     /translation="MGRFIFKGTSLIKLMLAFTLLIGALILVTPANAKAVNRDINPII
                     NKANPTQMAYWVRYRYWGPYYYRGYYYRPYYYRWGYPYYRPYYRPYYRPYYYYRW"
     gene            complement(4923..5747)
                     /locus_tag="U6309_001237"
     CDS             complement(4923..5747)
                     /locus_tag="U6309_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504686.1"
                     /translation="MNEKLIETQSNLIKQAEAELTQSNQLLESLQQEKGLQEESDLDE
                     LDSLKSLILVLQYRLDTQNALSQLGHLQQYLLQLLGIEAKHSFEFNLERLAFALGTDE
                     LKQILAMLNHLIESLLRVIAQKQLKESHDKRKSLEQTKQFKPIDTLVKVLNKQKLFMA
                     KLEELKLVLENIGGAPQPGIIYDHIAALQGPVSRFYQALQHGLGLSNSLYQQLQKKNQ
                     LSYQLPDTLLKPEHVIRNMNFHPESQRLFKTTLERNKLENLEERAAAKRLGHFFNH"
     gene            5868..6992
                     /locus_tag="U6309_001238"
     CDS             5868..6992
                     /locus_tag="U6309_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN5504687.1"
                     /translation="MEQTSAQIVFEQKNATYQCCGSWSVLTLDGLPKKFSTSPLPDSQ
                     QIIINGEKISVFDSAGALALKKCIDELKKRNNQVELVNFSDSQHQMLELIESKQDILS
                     YQLPAVSKEGVLYQIGKESENKLHQVDGLIILIGDLSTRLFEAFAYWRRFHLPSIVSV
                     IDSAGLKALPIIGLLSFLIGVVLAYQMGLQLETYGANIFIAYLSGMAIFREFGPLITA
                     IIVAGRTSSSFTAQIGSMKINEEVDAILTMGLSPTELLVLPKVLGLLIVFPLLIFWAD
                     VFSIWGAMIMSKNMLNIGFEDFLTRLRDSVGLNQMMLGLYKAPAFSILIALVGCFQGF
                     RVESSADSIGSQTTKSVVQALFLIIIADAAFSIAYSWMGL"
     gene            6993..7730
                     /locus_tag="U6309_001239"
     CDS             6993..7730
                     /locus_tag="U6309_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN5504688.1"
                     /translation="MSEPVIEIKGMKNCLGGQWVHTDVNLTVERGEILAIIGGSGSGK
                     TTILRSLLMLLKPSAGSIKVFGEEIYNLDTEQAFRLRRRWGMLFQHSALFSAMTVLEN
                     VMFPMREFTTLDQEFMQELALLKITLVGLSKEAAVKYPSELSGGMQRRAAAARAIAMD
                     PELLFLDEPTTGLDPRGARLFDDLVVFLRDALNLTIVMVSHDIESLKRTTDRVAFVGD
                     GKILSVEPINELMKNKNPLIAEYFSKF"
     gene            7760..8683
                     /locus_tag="U6309_001240"
     CDS             7760..8683
                     /locus_tag="U6309_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEN5504689.1"
                     /translation="MESKTNYTIVGLIVLILTAGLLSAGLWLSVGFNQKEYTSYTVYL
                     KESVSGLSVESPVKFNGVQVGYVKEIKLNKNDPRQVELLLNIEKSTPITTSTSATLIT
                     QGITGVTYVGLSAGSSELTPLRKMPGEPYPVIPSKPSLLNQLDAVLKEVAENVGAVSE
                     KAQLIFNEENADNIRKSLANIERITEIIADKGQTIDSSLNNLDVFVANMAKASKQFPQ
                     LIKDLKTGISKFKSLADNMSAAGKDVSKTMIAGKNTIDQISQQAIPPAVILLRRLNAI
                     SANLEKVSNEMRQNPSVIIRGTKAPKLGPGE"
     gene            8687..9289
                     /locus_tag="U6309_001241"
     CDS             8687..9289
                     /locus_tag="U6309_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEN5504690.1"
                     /translation="MRVLSSVCFILGILTLMSCSPVKVPVTNEYQLTAYSTKQLTRKP
                     KPITIQVTVPEAVAGYQTEEMLYMKKPFKLEPFVKNAWTSPPADMLFPLLVQSLQSSG
                     YFYAVTSSPYSEEADYRLDTQLLKLEQNFIKKPSVLEFSAKIVLTHISDNQIIGSRIV
                     SLQIPCSQDTPYGGVIAANQAAFRFTATATDFVVSHIKRD"
     gene            9387..9917
                     /gene="pal"
                     /locus_tag="U6309_001242"
     CDS             9387..9917
                     /gene="pal"
                     /locus_tag="U6309_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEN5504691.1"
                     /translation="MKAGSFYKLGLLVASAVLVAACSKTPGSADGGAAVGDGDATAQG
                     LGQMTHFAGQEPGESYTTQAPHNQLYLFAYDDSTLASKYLPSVNAQAEYLKTHPGARV
                     MIAGHTDERGSREYNVALGERRADTVAEILRMAGVSRQQIRVVSYGKERPANYGHDEA
                     SHAQNRRVEFIYEATR"
     gene            9914..10882
                     /gene="ybgF"
                     /locus_tag="U6309_001243"
     CDS             9914..10882
                     /gene="ybgF"
                     /locus_tag="U6309_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEN5504692.1"
                     /translation="MINCKKSIITLCFVLMLPFASWAEAPVIDDSENFAMIDRQEEYD
                     APLVNPKYDDPQIENAELDGSQNDNYSTDTSQSYDEPALVKEDRSTISDNAKLIDKIQ
                     QLQKEIQELRGQLEVQAHDLKLLQQQQVAFYKDLDSRLSNSSTSAKTVQNDKPATAIS
                     LGSNSPETLKVASPQIKAGSSNSKPQPVVAVSRANPADEQISYLAAYELVKNKRYDEA
                     IKSMQTFVQKYPRGGYTANAEYWLGELYLVKKDYSKAIEHFEIVLQQYPSSSKAAASL
                     LKSGYAYAEKGDKQEAKKRFQQVVKTYPDTPTAQLASSKLEAINAL"
     gene            10879..11532
                     /gene="queE"
                     /locus_tag="U6309_001244"
     CDS             10879..11532
                     /gene="queE"
                     /locus_tag="U6309_001244"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEN5504693.1"
                     /translation="MKRFNEQLRITEIFHSLQGESITVGLPTVFVRLTGCPLRCQYCD
                     TAYAFSGGEVVEIDDILNKVASYQCQHVCVTGGEPLAQPGCIPLLSKLCDAGYSVSLE
                     TSGARDIASVDQRVMIVMDLKTPDSRETDKNLLSNLSFLKPNDQIKFVLCSRNDYEWA
                     CSMLSEYQLAERVQLLFSPSWNQLNPTDLANWIIQDKLPVRFQLQLHKILWNDAPGH"
     gene            11550..12497
                     /locus_tag="U6309_001245"
     CDS             11550..12497
                     /locus_tag="U6309_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEN5504694.1"
                     /translation="MHWFAQAPANIALIKYMGKKDENSNLPDNSSLSYTLSNLLSSVK
                     LEKLPTKKDIWEPLSIPGAPEFNLSVEAQKRFIDHLVRLKEYFGYVGGFLIQSSNNFP
                     HSSGLASSASSFAALTKCASIALSELTQKPLPSIDEQAQLSRLGSGSSCRSFYAPWAL
                     WTGDKVSAIDLPYKDLLHQVIVISSQEKEIPSRVAHKLVKTSPFYETRSERAEANLKL
                     LLNAFENKDWTSIYQICWHEFLDMHQLFKTCEKPFSYITDNTLHILSVIEKFWNEKGD
                     GPIVTMDAGPNVHLLYRSDQTDLARQFKSDHLVGNYDVL"
     gene            12487..13365
                     /locus_tag="U6309_001246"
     CDS             12487..13365
                     /locus_tag="U6309_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEN5504695.1"
                     /translation="MFYDFETTTFGKWILAGEHAVVRGHEALVFPIKERQLTLKYNPS
                     SPSLSAEFQGINGSDMHLLFWSVLERGMHLLGRSLNELGGHFYLETNIPVGVGMGASA
                     ALCVAMSRWYCAQQMIQDNQCNEFAKQLEHLFHGKSSGLDIAGVASDTGVYFKSGLCT
                     PVKQTIYPYWYLSSCNQIGITSHCIQQVEYLWNKNPELAHQVDKQMVEAVQEAKAVLE
                     EGGSNALKRLAKAINKAADCFLQWGLVSESLQQHMNHLFADGAIAVKPTGSGGGGFVL
                     SLWDKIPSSHTELIPV"
     gene            complement(13378..13647)
                     /gene="ftsB"
                     /locus_tag="U6309_001247"
     CDS             complement(13378..13647)
                     /gene="ftsB"
                     /locus_tag="U6309_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEN5504696.1"
                     /translation="MRPIFIILIVALVALQHKLWLGDGNIIQWIKLEKKLEAHKSQND
                     KLAARNKALEADIKELKSGDQALEEQARYELGMIKQNEVYYQFVD"
     gene            complement(13869..15137)
                     /gene="eno"
                     /locus_tag="U6309_001248"
     CDS             complement(13869..15137)
                     /gene="eno"
                     /locus_tag="U6309_001248"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEN5504697.1"
                     /translation="MHIHKIQAREILDSRGNPTIEADVILTTGIIGRASVPSGASTGS
                     REACELRDNDPKRYAGKGVQKAVKHVNNEINQALQGLSVEDQENLDRILCQLDNTENK
                     SHLGANAILATSLACARARALSLNQPLYMTLNQGDMMTMPVPMMNILNGGAHADNNVD
                     IQEFMIMPIGAPDFPVALQMGTEIFHVLKSVLKKQGLNTAVGDEGGFAPNIQSNRQAL
                     DLLSEAIEKAGFRLGEDIVFALDVAASELFNEGFYHMYSENQKFDSHQLIEYYANLIS
                     SYPIVSIEDGLDEKDWSGWKQLTTHLGNKVQLVGDDLFVTNPKILREGIAQGIANAIL
                     IKVNQIGTLSETRQAIKLAYDNGYRCVMSHRSGETEDTFIADLAVASGCGQIKTGSLC
                     RTDRTAKYNQLLRINELASLPYAGKNILKR"
     gene            complement(15169..15771)
                     /gene="maf"
                     /locus_tag="U6309_001249"
     CDS             complement(15169..15771)
                     /gene="maf"
                     /locus_tag="U6309_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEN5504698.1"
                     /translation="MSKFLQQKPIILASSSTIRHKLMKSLGLDFLVVPSNCNEEEIKT
                     RHNSDELVELGITLAKIKALDVSQHYPEHYIIAADQLCVADKRVFNKPLNHQTAVSHL
                     RELSGKQHQQIACLCIVKESKILWQYHETATLTLHHLSEKTIEAYLQAEKPYQSCGAY
                     QYEGLGKWLFKEVQGSEDTILGLPLMPLVNALVNLKVVGI"
     gene            complement(15764..16522)
                     /locus_tag="U6309_001250"
     CDS             complement(15764..16522)
                     /locus_tag="U6309_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEN5504699.1"
                     /translation="MLSATSLKIIFAVSIFIVILIAGWYPFKKRLKDDKHIDFPIGET
                     LATGVFLGAALLHMLPESNTLFKEMGYNYPFAFIITGVVFLIFLWFEHLGKELYHHHD
                     AEHPAFAILAWAMLSVHSLMLGAALGFTQYNSMIIMLFLAIITHKWAESLAIAIQLNK
                     SSMSTRKSMVFFFFFSLMTPLGIYFGWHFGHGVETNSLFDPILIAASAGTFLYLGTLH
                     GLERCVMVERCCNLSDFSFVIIGFLLMASVAIYV"
     gene            complement(16638..17780)
                     /locus_tag="U6309_001251"
     CDS             complement(16638..17780)
                     /locus_tag="U6309_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN5504700.1"
                     /translation="MYPHDRYWQAKKVTLIGAFINALLGVVKMIGGFLYHSHALIADG
                     VHSISDLITDVMVVFASKYGSVSADDSHPYGHQRIETAATLLLSLLLILAGCGIAWDA
                     LDELLKSDHTMPNWLSIPIVCFSIIANEALFHYTRHVGKKISSQLITANAWHHRSDAA
                     SSIVVLIGLIGSLAGFVYLDAIAAVIVSLMIIKMGWSYGWNSVKELVDTAVDAELFSQ
                     IERVIQSIDGVKRIHQLRSRFMGSDVLIDVHILVSPKISVSEGHYIAQHVHHTLVKEI
                     DCVKDVIVHVDPEDDEISCPSLHLPNRAILQEKVFNDIRHDFPQILFWNIHYLNGKMS
                     LDIFCSNDFNQWKKLHNRVISTLKSQIDIKEVRLFGLHEEMHHPSN"
BASE COUNT         5448 a         3362 c         3630 g         5620 t
ORIGIN      
        1 ctgtagctaa aattaaccag ttttgatctt gaactttatc tttgagagat tgtaagtctc
       61 ttttatcggt aagaattaac aaagtatcag ctcctaaagc accacagcct tttattgcca
      121 aggtttccgg ataagatttt atagactgaa tcagaggcag actatgttga ctcaccagat
      181 ttaattctgt caatttttga gcatataagt taatacattg gattaaatgg tggctatgag
      241 ccaaatcaaa agcttctttt gctttgataa ccaaagaggc gaggtaatct atttgagatg
      301 gcagggtgac ttcttgaaga tgatgatgtg tcgccaattt gttgccggtg tgaatgatta
      361 agaaggataa atctttgaat ggccaggaga acgaattaac ctgatttttt tgtttatcaa
      421 tgtaaacaca accttgctgt gactgagcaa tcacatcata accactgggt cttaacccca
      481 accccgacca ggacgattgg taataggcct caagcatata gtttaatcga ggggtaatct
      541 cttgcagtga acaagttgcc aaataacagg ctaaaaattg cgcgctggat gcgcccaaac
      601 cgccttggcc atgataaggg tcttgccagg atagaccaaa gggatagcat tgattttttt
      661 gccaccataa tccagcaggt gactccggat gaattccttc taattttgca ttttcagtta
      721 atgtaagaat aaagcaaggg cttgtcgtta ataggatagc tggttctccc gcaatggcag
      781 cgtattctcc taataaaaat gttttagcgg gaatttgcca tttcatactg caacaggagc
      841 ttgtcggatt tcagctaata ggtcataagc gttattcaag ctgactcgtt tgttcatgtt
      901 aagccattct tgcaattttt ctttgagcac gggcatttca ttttcattag cccctgcaac
      961 taatagcaaa ttatcaatat gcaatttcat atggccttga atgatcccgt cagtgcataa
     1021 agctttgagt gcgccaagat tttgcactag tccaactgct gcgatcacct gggagagttg
     1081 attggcagaa gtgatatcca tcattcgtaa acacatttta gccgtggggt gtaaagaggt
     1141 tacgcctcca acagtaccta ctatgatagg agcagttaac tctcctgtga gtatttcatt
     1201 ttgataccgc cagcgagtaa tcgctttgta ttgtcccgaa cgtgccgcat aggcgtgtat
     1261 tccagcttcc acagctcgcc aatcattccc tgtagcaatt aaaaccggat caattccatt
     1321 cataacccct ttattatggg tagcagcgcg atagggatct atttctgcaa agagtgaggc
     1381 ctcttgcaat ttttgaccga ggatggggtc aatattacga atgatgactt gagcggtcgt
     1441 taatttttga tcgtttaaat tggataaaat acacatggtg acttcttcac cagttatttg
     1501 ttctatgggt tgttttaaat attcaagtac ttgattgatg atattggcac ccatagcatc
     1561 gcagctgttc atcgtcaaat gaattactgc catatcttgc ccatcttctc tctttaaatg
     1621 acgtacttgc aaatcagtaa caccgccgcc tcgtttgacc atattggcgg caacgtcttt
     1681 attcgctatt tctataaaat attgacgatt tttattaaag aggtcagaga atctttgaaa
     1741 gtcctttact tttgccagtt gaatttgccc aagaatacat tctccgtgaa cccaggtatt
     1801 gatttcacca tgctgcctaa tccatttcgc tgatttggaa agagcagcaa taatagaggt
     1861 ttcctccact gccaaaggta ttacataatc acgtccatca atattaaaat tagtagccac
     1921 acccaatgga agctgaaaat atccaatcac attttcaatc aatttgtcgg ccagatttaa
     1981 atctttaatg ccgccttgtt taagaaatgt tatgtcttca tgagtcaagg cacctaaagc
     2041 gcataacctt tgaaagcgct cttcacgtga taactttgag aatccgcgaa ataattcacc
     2101 ggcattagca gctatggaca tgccttctcc tttaaatcat caagaactcg gctgccagta
     2161 caaaacatag cagttttcaa ttcatattca attgtattca tttgggtcag tacttgcccg
     2221 gttgagtcca aagccgcttc aagcatgggt ttcgcgaagc cgactgtggt agcgcccagc
     2281 gcaaacagtt ttgcagcatc caaaccattt cttacgccgc cagaacccca agtttcgaaa
     2341 gagggattaa gactaatggc gttacgaatg ctttgtaaag tatctatgcc ccaatttcga
     2401 aaagtatcag cagttcggtg tcttatcgga tccttatttg cccgatgtcc ttcaattcgc
     2461 ccccaatgcg tcccccccac accactgacg tcaacagcgg caacacctat attatttaag
     2521 cggagcagcg tattttttga aaaaccacaa ccagtttctt ttattataac tggagaagca
     2581 atctttttga ctaatgcttc caatgctgcc cagcatcctt gaaaattagt tgttccttca
     2641 ggttgaatgc actcctgtaa tggattgcaa tgaataatca atgcttcagc ttgcaatgta
     2701 tcaattaagc gctcgattgc agaaattgga gtgtcaatta actgggcgat tcccagatta
     2761 ctaaacaggc tgaccatggg gaaatcacgg cggagtggcg cccactcaaa tgcggcttgt
     2821 ttatcggtta attctctgcg ttgtgagcca acgcccattg cccattttgt ttttgagcac
     2881 gcttccatta atcgatagtt aatttctaga gcattggaat ggcctgctgt cattgaactg
     2941 ataataaatg gtttttcaac cggtttttta aatcggatgc tttgtatgga aatgtcttta
     3001 aaatcaaggt caggtaaggc ttcatgcacc aaagaaaaat gatcaaaggg atttaattcg
     3061 ctgctttgat tggctggcat taaggccagt tctatgtgat cgcgcttacg ctgttcaaat
     3121 tggctgtatt cgttttgcat gcctgaattt acttttgaaa aatagcgtca attttagcat
     3181 aaaatcatga aatagaccat tgcatttcta ttcgacatag tgatttattt ggtatcattg
     3241 atttaaccat gagttaacta attttttcgt ttagttggct ttttggtaac aaattaaggt
     3301 aattatcatg aaatctaaac ttgaatacca tttctctgca aacgcggatg gcgatgaatc
     3361 ctgtattgaa gatgttgatg gaaatgaatt tactccaaat ggttcaccat cacatggatg
     3421 gactgcgtgg actagatttt ttggccatca aaaccagtct cctaaatatg ttgttaaaca
     3481 acctagacca gataaagtcc gttcctcttc agatcttaga gataaaaaac ttaataatga
     3541 atggaacaga acacgctatc agcatgaact ggatatgtgg aatttggttt atccagataa
     3601 taaagcctat ttgtttacag ataacggaat tcgattggtt ctcccttatt tacccggtaa
     3661 aactctattt tcttttacta gtagggaaga agatcagtta acttggtgtc aactaaacta
     3721 tgcagtattt tgtgagttta agcgctttat tgaacttgga ttgaagcatt gcgatgtaaa
     3781 atatgacaat tttttgatag aaagaaagca agatggtacc tttaaagcct acataattga
     3841 tctcgattca gtgggtaaac taaagcctaa tgatcaggat tatgattgga atattgtcct
     3901 ggcatctcta ttcgacgata ccggactaaa aagtccggat gtttgcccac ccggctatat
     3961 tgctttgaaa tcaatcgatg ttccagcaga aaatgagaat gaagcacgat caaaacttga
     4021 taaaataaaa aaagaactat cgcaaaaaaa acctaccttg gtaagaatca agaattgcca
     4081 gcaaaataag ttggattatt attttgttta tggatgtttg tataatgggc aatggggctt
     4141 tactaaactt gatgagaaga tcattagttc ggttaatcta tctaattttt ctggtgcttt
     4201 agttgattgt agagctgctt ctgttattgg taatcatatt aaattaaaac aaggccacat
     4261 tagttacaga cttgaccttt atatcaactg tctgaatgag aaaatagctg aactcactct
     4321 actcaaagct cacctaaaaa gctgaatatc tcattttaaa tcagatccag tttctatcat
     4381 gacgggatca aagcgtgctg ctaagctgta agtatagtat ctttgtcttg gtaatgcctt
     4441 cttaggctat atcaatatag ctatattcta tacataggcc atcagcgtat tatcgtctac
     4501 cagcgataat agtaataagg tctatagtag ggtctgtagt aaggtctgta atatggataa
     4561 ccccagcgat agtaatacgg tctatagtag tatccgcgat agtaatatgg gccccaatac
     4621 ctatagcgta cccagtaagc catttgagtc ggattggctt tattaattat cggattaata
     4681 tctctgttaa ctgctttggc atttgcaggg gtgactaaaa tcaaagctcc aatcagaagg
     4741 gtaaaagcta gcatcagttt tattaaagag gtccctttaa agataaaacg tcccattttt
     4801 tactccttaa agcgtacttt atttaagtat agactacacg ggattatttg gttaatattt
     4861 gcacataatc attactgggc atgagtttaa attttattga ggcagtaagt ggtttattag
     4921 gattaatgat taaaaaaatg cccgagcctt ttggccgcag ctcgttcttc aaggttctct
     4981 agcttattgc gttccaaggt tgttttgaat agtcgctgtg attcgggatg aaagttcata
     5041 ttgcgaataa cgtgttctgg tttaagcaag gtatcaggta attgataact caattgattc
     5101 tttttctgta actgttgata aagagagttg cttaagccca agccatgttg tagcgcctgg
     5161 tagaaacgtg atacgggccc ttgtaaagcg gcaatatggt catagattat gcctggttgt
     5221 ggagctccac caatgttttc aagaaccagc tttaactcct cgagtttcgc cataaataat
     5281 ttttgtttat tcaaaacctt aaccaaggta tctattggtt tgaattgctt ggtctgttca
     5341 agtgatttcc ttttgtcatg agattcttta agctgttttt gagcaataac acgtaataac
     5401 gactctatca aatgattaag catagccaga atttgtttta attcgtctgt tcccaaagca
     5461 aaagctaatc gctccagatt gaattcaaaa gaatgcttgg cttctatccc taataattga
     5521 agcaggtatt gttgtaaatg acctaactgg cttaaggcat tttgtgtatc cagtctgtat
     5581 tgcaaaacca gaataaggga tttgagtgag tccaattcat ccaagtcaga ttcttcctgt
     5641 agtccttttt cctgttgtaa agactccagc agctgattag attgagtgag ttctgcttct
     5701 gcttgtttga tgaggttaga ttgggtttca attaactttt cattcatgaa gataacctgg
     5761 atataaaaga gcactaccta tattattatg ataccatgaa tcgtaagttt ttgcggtctt
     5821 gtggtatttt gtattattct aggaaaattc aataaaaaga aataaatatg gaacagacta
     5881 gcgcacagat tgttttcgag caaaagaatg caacatacca gtgttgtggt tcctggtctg
     5941 ttttgaccct tgatggatta cctaaaaaat ttagcacaag cccattacct gattcacaac
     6001 aaataatcat aaacggtgaa aaaatcagtg tttttgatag tgccggagct ctggcattaa
     6061 agaaatgcat agatgagctt aaaaaaagaa ataaccaggt tgaattagtc aatttttccg
     6121 atagccaaca tcaaatgctg gagctcattg agtccaaaca agatattctt tcctatcaat
     6181 tacctgctgt ttccaaagaa ggtgttcttt atcagattgg taaagaatca gaaaataaat
     6241 tgcaccaggt tgatggtttg attattttga taggagattt aagcacaagg cttttcgaag
     6301 cgtttgcata ttggcggcga tttcatttgc ccagcattgt ttctgttata gattctgccg
     6361 ggctaaaagc gttacctatc attggattat tgtctttttt gataggggtc gttttagctt
     6421 atcaaatggg tttgcaatta gaaacttatg gtgccaatat ttttattgcc tatttatctg
     6481 gcatggcaat ttttcgggaa tttggacctt taattacagc aatcattgta gcggggcgca
     6541 ccagctcatc attcacagct caaattggca gcatgaagat taatgaagag gtagatgcca
     6601 tcttgaccat ggggctttct cccactgaat tattagtttt acccaaagtc ttgggtttat
     6661 taatagtgtt tcctttatta attttttggg cagatgtctt tagtatttgg ggggcaatga
     6721 tcatgtccaa aaatatgtta aacattggtt tcgaagattt tttaactcgc ttgagagatt
     6781 ccgtaggact taaccaaatg atgctgggtt tgtataaagc ccctgccttc tcgatattaa
     6841 ttgctttagt gggttgtttt caggggttta gagtagaatc aagtgctgat agtataggaa
     6901 gccaaacaac caaaagtgtt gtgcaagctt tatttttaat tattattgct gacgctgctt
     6961 tctccatagc ttatagctgg atgggtttgt aaatgagtga accagttatt gaaatcaagg
     7021 gaatgaaaaa ttgtcttggc ggtcaatggg tgcacaccga tgttaatctc accgtagaaa
     7081 ggggagaaat cctggctatt ataggaggct ctggaagtgg taaaacgacc atattacgca
     7141 gtttgctgat gttattgaaa ccttctgcgg gtagtattaa agtattcggt gaagaaattt
     7201 ataatcttga tacggagcaa gctttcaggc ttaggcgccg atggggcatg ttatttcagc
     7261 acagtgcttt attttcagct atgaccgttc tggagaatgt gatgtttcca atgagggaat
     7321 tcactacttt agatcaggag tttatgcaag aactggcact tttaaaaata actttagtag
     7381 gcttatctaa ggaagcagct gtgaaatatc cttccgagtt aagtggggga atgcaaagaa
     7441 gagctgccgc ggcaagagcc attgcaatgg atccagagct attatttctg gacgaaccta
     7501 caacaggatt ggatcctagg ggagccaggc tttttgatga tttggttgtt tttttaagag
     7561 atgcgttaaa tttaaccata gtcatggtta gtcatgatat agagtcttta aaaagaacaa
     7621 cagatcgtgt tgcatttgta ggtgatggga aaattctcag tgttgagccc attaatgaat
     7681 tgatgaaaaa taaaaacccg ctaatcgctg aatactttag caaattctaa gaaacactat
     7741 aacagagggg acaacttact tggaatctaa aaccaactac accatagtag gcctcatcgt
     7801 acttattctg acagctggcc tattgtctgc gggtttatgg ctctctgtcg ggtttaacca
     7861 gaaagagtat acttcttata ctgtttatct caaggagtct gtttctggtc tcagtgtcga
     7921 atcgcctgtt aaattcaatg gggtgcaggt tggttatgtc aaggaaatta aactaaataa
     7981 aaatgatccc aggcaagtgg aattattgtt aaatatcgaa aagagtacgc caataacaac
     8041 cagtacttcc gcaaccttaa ttacacaggg tattacagga gttacttatg ttgggttatc
     8101 tgctggatct tccgagctca ctcctttacg aaaaatgccg ggggagccct atcctgtaat
     8161 tccttccaaa ccatctcttc tgaatcagtt ggatgctgtt ttaaaggagg ttgctgaaaa
     8221 tgtaggtgcg gtgagtgaaa aggctcaact tatctttaat gaagaaaacg ctgataatat
     8281 aagaaaatca ttggctaata tagagcgtat tacagaaata atcgctgata aaggtcaaac
     8341 aattgatagt tctttaaata atcttgacgt gtttgtagcc aatatggcga aggcaagcaa
     8401 acaatttcct cagttgataa aagatttaaa aacaggtatt tcaaaattca aatctttagc
     8461 tgacaatatg agtgctgcgg gtaaggatgt ttcaaaaacg atgatagcgg gcaagaatac
     8521 tatcgatcaa atttcccaac aggctatacc tccagcagta attttattgc gacgcttgaa
     8581 tgccatttca gcgaaccttg aaaaggtcag caatgaaatg aggcaaaacc cttctgtgat
     8641 tattcgaggt acgaaagcac ctaaacttgg gccaggggaa taaaatatga gagttctcag
     8701 tagtgtttgt tttatactag gaatattaac actgatgagt tgttcacctg ttaaagtccc
     8761 agtgaccaac gaatatcaat taacagcata cagtaccaaa caattgacca gaaagcccaa
     8821 gcccataaca atacaagtta cagtgcctga agcggtagca ggatatcaga ccgaagaaat
     8881 gctttacatg aagaaaccgt ttaaattgga accatttgta aaaaacgcct ggacctcacc
     8941 tcccgcggat atgctttttc ctttattagt acaaagcctg cagagttcag ggtattttta
     9001 tgccgtcaca tcgagtccct atagtgaaga agcagattat cgattggata ctcaactatt
     9061 gaaattagag caaaatttta tcaagaaacc tagtgttctt gaattttctg cgaaaatagt
     9121 actaacccat attagtgata atcaaattat aggttcgcga attgttagtt tacaaatacc
     9181 ttgttcacaa gatactcctt atggcggagt tattgcggcg aatcaggctg catttcgatt
     9241 tactgcaacg gcaacagact ttgttgtatc gcatattaaa cgtgattagg aatagccaaa
     9301 aaataaaaag aattgtacaa tggctctcca tgattatcat gtgtagtttc taaaaaataa
     9361 taagaagagt ttaaggagat tgtggaatga aagccggatc gttttataaa ctgggactgc
     9421 ttgtagcaag cgctgtattg gttgcagcct gttctaaaac cccaggcagt gcggatggtg
     9481 gggctgcggt aggtgacggt gatgctactg cccaaggttt ggggcaaatg actcactttg
     9541 ctggacaaga gcctggtgag tcctatacaa cgcaggcacc gcataatcaa ttgtatttgt
     9601 ttgcatatga tgacagcact ttggcatcaa aatatttacc ttcagttaac gcgcaggcag
     9661 aatatttaaa gacccaccca ggtgctcgag tgatgatagc tggacatact gatgaacgtg
     9721 gtagtcgtga atataacgtt gctcttggcg aacgccgtgc tgatactgta gctgaaattc
     9781 tgcgtatggc aggtgttagc agacaacaaa ttcgagtagt tagctacggc aaagagcgcc
     9841 cggctaatta tggccatgat gaagcgtcgc atgcgcaaaa tcgacgcgta gagtttattt
     9901 atgaggcaac aagatgatta attgcaaaaa atccattatc accctatgtt ttgtactgat
     9961 gcttcctttc gcctcatggg cagaagcgcc agtaatagat gatagtgaaa attttgcaat
    10021 gattgacagg caagaggagt atgatgctcc tcttgtcaat cccaagtacg atgatcctca
    10081 aattgagaat gctgagttgg atgggtctca aaatgataac tactcaaccg atacctctca
    10141 atcctatgat gaacccgcac ttgtcaaaga agaccgcagt actatcagtg acaatgcaaa
    10201 gttaattgat aaaattcagc agcttcaaaa ggaaatacaa gagttacgtg gccagcttga
    10261 agtacaggca cacgatttga aattattaca gcagcagcaa gttgctttct acaaggatct
    10321 ggattctcga ttgtccaatt cttcaacttc agcaaaaact gttcaaaatg ataaaccagc
    10381 aacagctatt tcattgggtt ctaattcgcc agagacttta aaagtggctt ctcctcaaat
    10441 caaagcagga tcatctaata gtaaacctca gcctgttgtt gctgtttcca gggctaatcc
    10501 tgctgatgaa cagataagct acctggctgc atacgagtta gttaaaaata aacgctatga
    10561 tgaagcgatt aagtctatgc agacttttgt gcaaaaatat cccagaggtg gttacaccgc
    10621 gaatgcagag tactggctag gtgaattata tcttgttaaa aaagactatt ctaaagcaat
    10681 agaacatttt gagattgttt tacagcaata tccttcatcc agcaaggctg cagccagttt
    10741 gttaaaatct ggttatgctt atgccgagaa aggtgacaag caagaagcta aaaagcgttt
    10801 tcaacaagtt gttaaaacat atcctgatac accaacagca caattggcca gctctaaatt
    10861 ggaagcaatt aatgctttat gaaacgattt aatgaacaat taagaattac ggagattttt
    10921 cattcattgc aaggagaatc aataactgta ggcttgccta ctgtatttgt tcgcctgaca
    10981 ggctgtccat tgcgatgtca gtattgtgat acggcttatg ctttttcagg tggtgaagtt
    11041 gttgaaatag atgatatttt gaataaagta gcttcatatc aatgtcagca tgtttgtgtt
    11101 actggtgggg aaccacttgc tcaacctggc tgtattcctt tattaagcaa actttgtgat
    11161 gcagggtact ccgtctcttt ggaaaccagt ggcgccagag atatagcctc tgtagatcaa
    11221 cgagttatga tcgttatgga cttaaaaact cctgattcca gggaaacaga taaaaacttg
    11281 ctgtccaatt tgagttttct gaagcccaat gatcagatta agtttgtact atgcagccga
    11341 aatgattatg aatgggcatg tagtatgctg agtgaatatc agttggctga acgggtacaa
    11401 ctcttatttt ctccgagttg gaatcagcta aatcctactg atcttgcaaa ttggataatt
    11461 caggataaat taccagttcg ttttcaacta caattacata aaattttatg gaatgatgca
    11521 cctggtcatt gacctcaagg agactaacta tgcattggtt tgctcaggca ccagctaata
    11581 ttgctttgat caaatatatg ggtaaaaagg atgaaaattc caatttacct gataattctt
    11641 ctctatctta caccttgagt aatttattaa gttccgtgaa attagagaag ttgccgacta
    11701 aaaaagacat ttgggagcca cttagtattc ctggagcacc agaatttaac ctgtcagttg
    11761 aagcgcaaaa acggtttatt gatcatttag tacgattgaa agagtatttt ggatatgtcg
    11821 gtgggttttt aattcaatcc agtaataatt tccctcatag cagcggttta gcgagttctg
    11881 cctccagctt tgctgccttg acaaagtgtg catcaattgc attaagtgaa ctcactcaaa
    11941 aacctttgcc ttctattgat gagcaagcac aactgagtcg attaggttca ggctcatcat
    12001 gccgttcttt ttatgcacca tgggcgttat ggacgggcga taaagtctct gcaatcgatt
    12061 tgccttataa agatctatta caccaagtca tagtaattag cagtcaggaa aaagaaatac
    12121 catcaagggt agcgcataag ctggttaaaa caagtccttt ttatgaaacg cgaagcgaaa
    12181 gggccgaggc aaatttgaag ttgttactga acgcttttga aaacaaagat tggacgagta
    12241 tataccaaat ttgctggcat gaatttctcg acatgcatca attatttaaa acctgtgaaa
    12301 aaccattttc ttacattaca gataatactt tgcatatatt gagcgttatt gaaaaatttt
    12361 ggaacgagaa aggtgacggc cccatagtta ctatggatgc tggtcctaat gttcatttgc
    12421 tttatagatc agatcaaaca gatctcgcac gacaatttaa atcggatcat ttggtaggaa
    12481 actatgatgt tttatgattt tgaaaccacc actttcggca agtggatttt agcaggtgaa
    12541 cacgcggtag taaggggaca tgaagcttta gtatttccta ttaaagaaag gcaattaact
    12601 ttgaaatata acccttcctc accgtccttg agcgcagaat ttcaaggcat taatggttct
    12661 gatatgcatt tattattttg gagcgtatta gagcgtggca tgcatttgtt gggacgttca
    12721 ctgaatgaat taggcggtca tttttatttg gaaaccaata ttcctgttgg ggttggcatg
    12781 ggtgcttcgg cagcgctttg tgttgccatg agtcggtggt actgtgcgca gcaaatgata
    12841 caagataatc aatgtaatga atttgccaaa caattagaac atctgtttca tggtaaaagt
    12901 agtggcttgg atatagctgg tgtagcatca gatactggtg tatattttaa atccgggctt
    12961 tgtactccag taaagcaaac catttatccc tattggtatt tatcctcctg caatcaaatc
    13021 ggcattacct ctcattgtat acagcaagtt gaatatttat ggaataaaaa tccagaactt
    13081 gctcaccaag tagataagca aatggtagag gctgttcagg aagcaaaagc tgttttggaa
    13141 gaggggggga gtaatgcttt aaagcgtttg gcaaaagcga taaataaagc cgcagattgt
    13201 tttttacaat ggggtttggt tagtgagagc ttgcaacaac atatgaatca tttgtttgct
    13261 gatggtgcaa ttgctgttaa gccaaccggt tcaggtggtg gagggtttgt attaagcctt
    13321 tgggataaaa ttccctcatc tcatacagag ttgattccgg tctagctcct ttcaagccta
    13381 atcgacaaat tgataatata cttcattttg cttaatcatg ccgagttcat atcgagcttg
    13441 ttcttcaaga gcctgatcac cactttttaa ctctttgata tctgcttcca acgctttatt
    13501 tctggcagct aatttatcgt tttgactttt atgagcctcc aatttttttt cgagtttaat
    13561 ccattggatg atatttccat cacctagcca tagcttatgt tgtaaagcaa ctaaagcaac
    13621 aattaagata atgaatatgg ggcgcataga attttctatt gaagaatgta tctaattact
    13681 attgtagtat cttattacca cgaggggcaa tatgtgttca gggtatatag tattaaattt
    13741 tttatcgaac tgattttaaa ttgtatttta tttatattcc ctttgattta aattagagct
    13801 gatgctgagt tttattcagc actcaacatc accccaactt aaacattaat aatgctgaaa
    13861 gcttgaactc atctctttag aatgtttttt ccagcatagg gtaaagacgc caattcattg
    13921 attcttagta gttgattgta tttcgcagtc ctgtctgttc gacacaaaga tccggttttt
    13981 atttggccac aaccactggc cactgcaaga tcagcaatga aggtatcttc tgtttctcct
    14041 gagcgatggg acatgacaca tcggtatcca ttgtcgtatg cgagttttat agcttgcctg
    14101 gtttcgctta aagtacctat ttgattgact ttaattaata tggcattagc aattccctga
    14161 gcaattcctt ctcgcagaat tttgggatta gttacaaata aatcatctcc gacgagctga
    14221 actttatttc ccaaatgggt ggttaattgc ttccatcctg accagtcttt ttcgtccaac
    14281 ccatcttcaa tactgactat gggataactg gaaataagat ttgcatagta ttctatgagt
    14341 tgatgagaat caaatttttg attttcagaa tacatatgat aaaagccctc attaaaaagc
    14401 tcagaagctg caacatccag ggcaaataca atgtcttccc ctaatcgaaa gcctgctttt
    14461 tcaattgctt cgctaaggag atccagcgct tggcgattgg attgaatatt aggtgcaaaa
    14521 ccaccttcat caccaacagc ggtatttaat ccttgctttt ttaacacaga tttcagtaca
    14581 tgaaaaattt cagttcccat ttgcaatgca actggaaaat caggagcgcc gattggcatg
    14641 atcataaatt cttgaatatc cacattatta tcagcatgag cgcctccatt cagaatattc
    14701 atcataggaa caggcatggt catcatgtca ccctgattaa gggtcatata caatggctga
    14761 ttaagagaca atgctcttgc tctggcacat gccagagaag tagccaggat agcattagct
    14821 cccagatggg atttgttttc tgtattatcc aattggcata aaatgcgatc caagttctcc
    14881 tgatcctcaa ccgataatcc ttgtaatgcc tgatttatct cgttattaac gtgtttaact
    14941 gctttttgta cacccttgcc agcgtatcgt tttggatcat tgtctctaag ttcacaggct
    15001 tccctgcttc ctgtggaagc acctgaagga acgctggctc ttccaataat gccagttgtg
    15061 agaataacgt ctgcctcaat agttggatta ccgcgagaat ctaatatttc acgtgcctga
    15121 attttatgta tgtgcatgat ctttcctaat ctttttataa ttattaactt aaatacctac
    15181 caccttaagg tttacaagcg cattaactaa tggcattagt ggcaaaccaa gaatagtatc
    15241 ctcgcttcct tgtacttcct taaataacca cttgcctagg ccttcatatt gataagcacc
    15301 acaactctga taaggttttt ccgcttgtaa ataggcttca attgtttttt cacttagatg
    15361 atgcagagtc agtgtagctg tttcatggta ttgccaaagt attttgctct ctttaacaat
    15421 acacaggcaa gcaatttgtt gatgttgctt cccgcttaac tctcttaaat ggcttacagc
    15481 tgtttgatga ttcagtggtt tgttaaatac tcttttatca gcgacgcata attgatctgc
    15541 tgcaatgata taatgctctg gataatgctg gcttacatcc aacgctttaa ttttagccag
    15601 ggtaatccct aactcaacta gttcgtcgga gttatgcctt gttttaattt cctcttcatt
    15661 acaattcgac ggtacaacca ggaaatccaa gccaagtgat ttcatgagtt tatgtcttat
    15721 ggtcgaagag gaggcgagaa taatcggttt ttgttgtaaa aatttagaca tagatggcca
    15781 ccgatgccat gagtaaaaag cctatgatta caaagctgaa atcacttaaa ttacagcaac
    15841 gctctaccat gacacagcgc tccagtccat gtaaagttcc caaatagaga aatgtaccgg
    15901 cagatgccgc gattaatata gggtcgaata aagagttagt ttctactcca tgaccaaaat
    15961 gccaaccaaa ataaatgcct aaaggtgtca taaggctaaa aaagaaaaaa aataccatac
    16021 tttttcgagt gctcatcgag cttttattta actgaattgc tatagccaaa ctttccgccc
    16081 acttatgagt gatgatcgct aaaaatagca taatgatcat ggaattgtac tgggtaaaac
    16141 ctaaagcagc tcccagcata agcgagtgga cagaaagcat ggcccaggca agtattgcga
    16201 aagcaggatg ttctgcatcg tgatggtggt atagttcttt tcccaaatgt tcaaaccaca
    16261 agaaaataag gaatactact ccggtaataa taaacgcaaa cggatagtta taacccattt
    16321 ctttaaataa ggtgtttgat tcaggtaaca tgtgaagaag tgctgcaccc agaaaaacac
    16381 ctgtagccaa agtttcacct atgggaaaat caatatgttt atcgtcttta agtcgctttt
    16441 taaatgggta ccagcctgca atgagaatga ctataaatat cgaaacagca aaaatgattt
    16501 ttaaactggt tgcggacaac atgaagtctt cctactttaa aatgcgtata tattatcctg
    16561 aaatgactaa tcgaatctac agggcacagc agtattacat tgttaaaatt tagcaattct
    16621 tgcatacctt ctgaatctta gtttgatgga tgatgcatct cttcatgcaa gccaaagaga
    16681 cgcacttcct ttatatcgat ttgtgatttc agggttgaga ttactcgatt atgcaatttt
    16741 ttccattgat taaaatcatt tgagcaaaaa atatccaaac tcatttttcc atttaaataa
    16801 tgtatattcc aaaataaaat ctggggaaaa tcatggcgaa tgtcattaaa aactttttcc
    16861 tggagtattg ccctgtttgg taaatgtaag gatggacatg aaatttcatc atcttcaggg
    16921 tcgacatgga caatcacatc tttgacgcaa tcaatttctt ttaccagagt atgatgcaca
    16981 tgttgggcga tatagtgccc ctcggaaacg gatattttgg gtgaaaccag aatatggaca
    17041 tcaataagca catcactacc cataaaccgg cttctcaatt gatgaatacg ctttactcca
    17101 tcaatgcttt gtataactcg ttcaatttga gaaaataatt ctgcatcaac agctgtgtct
    17161 accaactctt tgacactgtt ccagccatag ctccatccca ttttgataat catcaggctt
    17221 acgatgacgg cggcaatggc atccaggtaa acaaaccctg ccaagctgcc aattaatcca
    17281 atcagcacaa ctatggatga agcagcatct gatcgatgat gccaggcatt ggcggtgatt
    17341 aattgggagc taattttttt tcctacatgg cgggtgtagt gaaataatgc ttcgtttgca
    17401 atgattgaga agcacactat aggaatcgat aaccagtttg gcatggtatg gtctgattta
    17461 agcaattcat caagtgcatc ccaggcaatc ccacagcctg ccaggattag taaaagggaa
    17521 agcaataaag tagctgccgt ttcaattctt tgatggccat atggatgaga gtcatcagcg
    17581 ctaacgctcc catattttga agcaaataca accatcacat cagtaattaa atcagaaatg
    17641 gagtggaccc catccgcgat gagagcatga gaatgatata aaaaaccacc aatcattttg
    17701 acaacaccga gtaaggcatt aataaaagca ccaataagag tcactttttt ggcttgccaa
    17761 tacctatcgt gggggtacat tgttgttcct tttcaatcac aaaaataatt tggatttttg
    17821 taaataaaaa ttagaaggga aactatacca tgttatagca tgagttccta tttatctcgt
    17881 gctggcagtt atagtcgact aaatgtatta ataatttctt tttggttttt actcgattac
    17941 gctaggagaa gttaaaaaaa taattgcaga tgttacccaa aagcaaattg gaaagttctg
    18001 aatcagttag aaaattgact gattttacaa ggtgttttgc attctgtgca caggattatc
//
LOCUS       DASLOE010000044        18030 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.044, whole genome shotgun sequence.
ACCESSION   DASLOE010000044 DASLOE010000000
VERSION     DASLOE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 18030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18030
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.044"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(39..1193)
                     /gene="lpxB"
                     /locus_tag="U6309_001252"
     CDS             complement(39..1193)
                     /gene="lpxB"
                     /locus_tag="U6309_001252"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEN5504701.1"
                     /translation="MPNASRVVIVAGEESGDHHAAELVKQLKAVYPNLEISGIGGKHL
                     RAAGVHLISDLTRYAVTGLTEIIPFLKIFHKAFQDIKQHLSTQKPDLLILVDYPAFNL
                     RLAKYAKKKLGLKIIYYISPQIWAWKGKRIHLIKDSIDKMAVIFPFEKTIYENAGVPV
                     SFVGHPLVKKIASAKDKHSSRTFLGLPLDEPIIALLPGSRHSEIERHIPILVNTAKLL
                     TLDNPKLRFVVPIAGTINPDKVKAYFSNQNLTVTFIQGQAIECMSAADFVIVASGTAS
                     LECALLEKPMCIIYKSSFLTYVAAMYFIKVKFLGLCNLLANKMMVPEFLQYDCNEIEL
                     SRYISNFHSDPNQPKSMINQLAKLKESLSSSQADCSLFDLVVAELPEKNA"
     gene            complement(1180..2142)
                     /locus_tag="U6309_001253"
     CDS             complement(1180..2142)
                     /locus_tag="U6309_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN5504702.1"
                     /translation="MNKIRCAVIGVGYLGRFHAQKYQLIPNAELVAVCDVNSTVCEAV
                     SSELNVPAYINYHDLFGKVDAVSIAATTNKHFEIAKFCLQHGIHVLIEKPITETVSQA
                     EELIRLAKQHQVKLQVGHLERFNSARLALDEYLELPLFIESQRLAPFNPRGTDVNVIL
                     DLMIHDIDIIQNIVQSPIKSITAQGAPVLTKAIDIANARISFANHCVANVTASRISFK
                     TERKTRIFQENSYISIDYQNKQLAVFKKGTGEMFPGIPDITRDQREFEKGDALLEEIR
                     AFLNCIENDSVPLVTGEEGRDALKTAEQITALINNNLVERYAEC"
     gene            2363..2938
                     /gene="rnhB"
                     /locus_tag="U6309_001254"
     CDS             2363..2938
                     /gene="rnhB"
                     /locus_tag="U6309_001254"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEN5504703.1"
                     /translation="MNTELKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKL
                     SPKQRNLLAIRIKEEALAFAYGRAEVEEIDQLNIHHATLLAMRRAVEALPIQPDNVVV
                     DGAFTPQLNIPCKAIVQGDSLIPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGY
                     ATPVHKEALMRLGPCKIHRRSYSPVADLISK"
     gene            complement(3049..4167)
                     /gene="rodA"
                     /locus_tag="U6309_001255"
     CDS             complement(3049..4167)
                     /gene="rodA"
                     /locus_tag="U6309_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEN5504704.1"
                     /translation="MSRQTTKPVYRFTAKSLHLDFPLLGLILTLIAFGLLILYSASNA
                     NMGMIMRQSMRLLFAFLIMFVLGFIPPHKYKIWTPWIYGVGLSLLIAVMLMGKIGKGA
                     QRWLELGLFRFQPSEIMKLAVPMMAAWFFDRQSHPSSIRSIGIASLIIFIPALLIAKQ
                     PDLGTAIMVTVAGLCVVFLAGIRFKIILLIALLMCSAIPVVWNLMHDYQKQRVYTLID
                     PEQDPLGAGYHIIQSKIAIGSGGLMGKGWLKGSQSHLNFLPEHATDFIFAVSGEEFGF
                     AGGFAIVALIVLISLRSLNIANNAQTTYTRLLSASLAMTFFLSAFVNIGMVMGIIPVV
                     GIPLPLVSYGGTAMVTFLASFGILMSISSHRILFNSLN"
     gene            complement(4164..6017)
                     /gene="mrdA"
                     /locus_tag="U6309_001256"
     CDS             complement(4164..6017)
                     /gene="mrdA"
                     /locus_tag="U6309_001256"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEN5504705.1"
                     /translation="MYRNQSFKNYRAESQHQRFRLNLLIAILIILSLILILRLAFLQI
                     SEFKRYQTLSLKNQMSVIPIAPPRGVILDRNGVLLAENIPVYVLEITPERVKDMKQTL
                     TKLRELIPSITDEDIDSFNKTRLQNRSFVPIPIKLKLSQEEVATFASNQYHFPGVSIK
                     ARLMRHYPLGEITAHALGYVGRINIQELKQVDSTNYRATNFIGKAGVEKYYEDILHGK
                     VGYQMVETDVSGRTLRIINKVNPHSGAKLYLSIDARLQEAAYNALKDKRGAVVVINSR
                     NGEVLAMVSSPSFDPNIFVSGVSKADYKVLSNALQRPLFNRAVRGVYPPASTIKPFVG
                     LAGLDKGFITTTTEIYDPGKYKLPTSSHVYRDWKKTGHGVINFKRAITVSCDTFFYQL
                     GNKMGISNIEDMLVKFGLGQLTHVDLHEEANGVIPSARWKRQTKGVSWYPGDTLISAI
                     GQGFMLATPLQMANATASLSQHGQRFRPHLLIKTVNSDTNETEEYKPFEEYPVSLRDE
                     ANWDVVIDAMHSVLTSNEGTGYRFGRNPPYPVAGKTGTAQVYSGRQYEKAKYEDIPEH
                     LRDNSLFIAFTPVEKPEIAIAVVVENDTIASTVARKVLDTYYQLYPIKKTP"
     gene            6251..7222
                     /locus_tag="U6309_001257"
     CDS             6251..7222
                     /locus_tag="U6309_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-box protein"
                     /protein_id="HEN5504706.1"
                     /translation="MKFTQDKFEKFDELTKDLQIETAQNLLNRGLVNLTQTSKPHLVL
                     FKPMVDVRKLLYCVVCGEHAKVQSMLEKDCSLIFKRGKVTDCSGREFESISAFEYVLW
                     ALDKHMWAAMIACIPSNEESRGVFVQLLAQYNKVNTDGVTYRLNGNTITEQHFDFENT
                     IIKELKTQVKEMKKQVDSVNAHNPTGDAIDNWDAIDNQWREGVGGAQKLLPMHIVDEY
                     CSNEPFYPVPQFTSQPKSSKQFYNWTTKKDEDWFNLNSKMGVDLAIYKGRGDRTLGCG
                     HVGIAASRDDLGAISALCEVRTMDFIKLKSLLEERATLDNYHQVVPM"
     gene            complement(7485..7955)
                     /gene="rlmH"
                     /locus_tag="U6309_001258"
     CDS             complement(7485..7955)
                     /gene="rlmH"
                     /locus_tag="U6309_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEN5504707.1"
                     /translation="MLKITIITLGNKMPDWVNSGVNEYAKRFHDGIQIKLIEIPLLRR
                     NKSSDLARILEKESALIKDALPANARLIALDMLGKSFSSEELALKLTQLQQISSHLCF
                     IIGGPEGLSNEILTLCDERWSLSKLTLPHPLVRIILLESLYRAWSIINNHPYHK"
     gene            complement(7964..8302)
                     /gene="rsfS"
                     /locus_tag="U6309_001259"
     CDS             complement(7964..8302)
                     /gene="rsfS"
                     /locus_tag="U6309_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEN5504708.1"
                     /translation="MLEKNPTLEKLLKSLEDIQAIDIKIIDVHKQTTITDFMVVTSGR
                     SSRHVKSIAQKVLEDMKVAGLPCLSSTGIEHGDWALIDFGDIILHVMQPEFRHFYNLE
                     GLWEEHPGNS"
     gene            complement(8407..9849)
                     /locus_tag="U6309_001260"
     CDS             complement(8407..9849)
                     /locus_tag="U6309_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEN5504709.1"
                     /translation="MEKHPKSLRIYFTTEMWERYGFYAVQSLLALYLALHFKWPDKEI
                     YSLVGSFTALTYLSPLVGGWIADKLIGQKRAILLGAIVLFLSYCMLSLIDNSLALTSS
                     LAGVAVGTGLLKPNISSLLGNEYPVGSTKRESGFTIFYMGITTGIILGTTLPSKFSEY
                     FGWSASFTSASIGLIIAFVVFLFGIHQYKIKDYNPFVFQPGKIISAFVLLVFLWSLSF
                     YILNSPQLANIMFGLVVLFSASYILYSVNRESANQSRQTLVIGLLCIISVIFWAFYFQ
                     MFMSLTLFIARVVEPSFLGIDFPPPYYITVQSIGMLIIGYFLAKKHHSLTMIERGLST
                     GKKFVLAMVFMTLAYSIIAFVSTVVDKSILISPLLIIPAYLMISLAELLLSPVGLSAI
                     TVLADKNKVSTMMGIFFVSLGIGGFLSGKLADLTAIPNGETNIIVLKTLYATAFTQQL
                     SILFIATLGCLVLFAVIKFLLTHIQICEKQ"
     gene            10072..11736
                     /locus_tag="U6309_001261"
     CDS             10072..11736
                     /locus_tag="U6309_001261"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEN5504710.1"
                     /translation="MSKDKNKRVISAPRGTEIQAKNWLTEAALRMICNNLDPNVAEDP
                     DSLIVYGGLGKAARNWECFDEIVHVLKLLNNDQTLLIQSGKPVGVFTTHEEAPRILIA
                     NSNLVPRWATWEHFNELDKKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVAAAKKHYQ
                     GDLSGRWILTAGLGGMGGAQPLAGTMAGASVLAVECDRQRIEKRLQTKYLDRYTDNLS
                     EALDWINESCRRKKPVSVAVLGNAAEIFPQLVKLGVQPSLVTDQTSAHDPLNGYLPLG
                     WTLERAVEMRKKSPEEVVDAAKKSMAVQVHAMLEFHNKGIPVFDYGNNIRQMAFEAGE
                     KNAFSFEGFVPAYIRPLFCEGIGPFRWVALSGDPEDIYATDERVKQLIPDDPHLHHWL
                     DMAREKISFQGLPARICWVGLKDRARLALAFNEMVKNKQVKAPIVIGRDHLDSGSVAS
                     PNRETEGMLDGSDAVSDWPLLNALLNCASGATWVSIHHGGGVGMGFSQHAGIVIVADG
                     TEKAAKRLARVLHNDPATGVMRHADAGYQIAKECAKENSLWLPMES"
     gene            11750..13270
                     /gene="hutH"
                     /locus_tag="U6309_001262"
     CDS             11750..13270
                     /gene="hutH"
                     /locus_tag="U6309_001262"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEN5504711.1"
                     /translation="MSEHFILQPGQLSLVSIKQILDEELSCVLAENAFELIRASHQTV
                     KKVIDEKKTVYGINTGFGSLANQTISSDCLKELQRNIVLSHACGTGKLLPDSVVALIL
                     LLKINNLSQGYSGVRLELINALIALFNHKVYPCIPSKGSVGASGDLVPLAHLSLPLLG
                     EGEVRHQGQVISAEEGLKLAGLKPLELEAKEGLALLNGLQVSTALALSALFISETLFE
                     TAIISGSLSVDAASGSDVPFDDRIHQIRGHQAQISAASMYRNLLAGSQIRESHRHCNR
                     VQDPYSLRCQPQIMGAVLHQMQFVGQTLQVEANAISDNPLVFAEQGDILSGGNFHGEI
                     IAMAADNLALALSEIGGSAERRIALLIDKNFSGLPAFLVRESGLNSGFMIAHVTAASC
                     ASDNKALAHPHSVDSLPTSANQEDHVSMATSAARRLHEMIDNTSTILAIELLAACQGL
                     EFHKPLKTSPQLDKIYQSVRGVVKEYDKDRYFAPDIEKIKKKILDKEFSLLTLTNE"
     gene            13284..14672
                     /locus_tag="U6309_001263"
     CDS             13284..14672
                     /locus_tag="U6309_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HEN5504712.1"
                     /translation="MELMAEFLTLHQQHKNNPYPSLSERKKLLIAIKKILQTEAYTLA
                     EAINKDFTHRPVEETLFLEVFPTIKAINFCLKNMKKWMRKRRRNVSWLFIPAQAYVIP
                     QPLGVVGILVPWNYPVYLALVPAIYALASGNRVMIKMSELSPHIGDTLQKLVHASGIN
                     HSISVINGDIELSKHFASLPFGHLMFTGSTKVGKLVMKAASDNLTPVTLELGGKSPAI
                     LSPTMNPAYFKRLFMGKLLNAAQTCIAPDYVLIPKGWEERVETEFKKFINTFYPDLMS
                     NEQYSSIISESHKKRLLDLVEDARSKGARVVEFGHLIPNSSKLPVFLLFDITNDMLVM
                     KEEIFGPVLPVLTYNSIFDAVDLINSFPNPLALYYFGEDKNEIKIIQTTTLSGALTIN
                     ETLMHIAIDDLPFGGVGHSGMGHYHGKEGFDTFSKLKPVLVQRFISTVSWLYPPYGAF
                     MRMFLAWVGGIK"
     gene            14684..15493
                     /locus_tag="U6309_001264"
     CDS             14684..15493
                     /locus_tag="U6309_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN5504713.1"
                     /translation="MNKVIFITGGSRGIGREIALKFAAKDKANIVIAAKTAEPHPKLE
                     GTIYSVAKEIEELGGQALPLMVDVRDEQQIQNAITKTIETFGRLDVLVNNASAINLTD
                     TLNTPMRRYDLMQSVNVRATFACSQAAIPYLLKSENPHILTLSPPLNMDKTWFAPHLA
                     YTISKYGMSMCTLGLAEEFKEAGIAVNSLWPKTTIATDAIRVHFPSELYMASRKPLIM
                     ADAAYWIINQPSKSITGNFFIDEDVLKNSGVTDFSCYAMNPKAQLCPDLFL"
     gene            15490..15921
                     /gene="rnhA"
                     /locus_tag="U6309_001265"
     CDS             15490..15921
                     /gene="rnhA"
                     /locus_tag="U6309_001265"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEN5504714.1"
                     /translation="MKVEIYTDGACKGNPGPGGWGVLLRYNGREKTLHGGEAQTTNNR
                     MELMAAIKGLEALKRPCEVDLYTDSQYLQQGMKEWIKTWKRNGWRNSKKELVKNAELW
                     KSLDNLASIHNIHWHWVKGHSGHLENDLVDALANLGIEELS"
     gene            15926..16627
                     /gene="dnaQ"
                     /locus_tag="U6309_001266"
     CDS             15926..16627
                     /gene="dnaQ"
                     /locus_tag="U6309_001266"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEN5504715.1"
                     /translation="MRQVILDTETTGIGPEQGHRVIEIGCIELIDRKLTGKHFHIYLN
                     PQREVDEGAFRVHGISTEFLQDKPLFQDIVTEFVQFVEGSELIIHNAPFDVGFLNSEL
                     KHVKWNKTLEDYCRILDTLILAREKHPGQRNSLDALCKRYEIDHFNRELHGALLDAEI
                     LAYVYLAMTGGQSSLFTEVESSLSQSKIKTQEIKSLSLKKPVIMKANEEELIQHQHFV
                     EFISKKSGINHWEEV"
     gene            complement(16834..17211)
                     /locus_tag="U6309_001267"
     CDS             complement(16834..17211)
                     /locus_tag="U6309_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504716.1"
                     /translation="MSRKLLIATTLVLSTSLFPLISNAEDTANPNEMTKDAWLNSMTP
                     LLPDLICKGFIQDPDLKKRFDEIKMTYEQCVTLIPESTKKCQDELYASMPDKINSETA
                     GTWGRSLGECIGKDFAEKHLIPK"
     gene            17503..17976
                     /locus_tag="U6309_001268"
     CDS             17503..17976
                     /locus_tag="U6309_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEN5504717.1"
                     /translation="MEKSNFRLNFLSQILLFTLGVFLFCNGYAAGNTFIFNPNTLTWK
                     AITANGKVIRTGRGSGGKHYCSDVRRSCKTPSGVFRVISKGGPGCRSSRYPVGKGGAP
                     MPYCMFFSKLYAIHGSPDVPNYNASHGCVRVKPQDARWLSQNFIKIGTKVIIKSY"
BASE COUNT         5593 a         3190 c         3754 g         5493 t
ORIGIN      
        1 atgaaggcta aatgataatc tagattttca tatcaaaact atgcattttt ttcaggcaat
       61 tctgccacta caagatcaaa aagagagcag tcagcctggc tggaagacag agattccttt
      121 aactttgcca gttgattaat catcgacttg ggttggttag gatcactatg aaaattggaa
      181 atatatcttg ataattcaat ttcattgcaa tcgtattgca aaaattcagg taccatcatt
      241 ttattggcta acagattaca taagcccaaa aatttaactt taataaaata catggcagca
      301 acataagtaa gaaaggacga tttgtaaata atgcacattg gtttttccaa taaggcacat
      361 tccaatgagg cagttccaga agctactatg acaaagtcag cagcagacat acattctatt
      421 gcctgtccct ggataaaagt aacagtaaga ttctgattgc tgaaataagc ttttacttta
      481 tcaggattga tagttccagc gatagggact acaaatcgaa gtttggggtt gtctagggtc
      541 agcaattttg ctgtattcac caatatcggt atgtggcgtt caatttcaga atggcgactc
      601 ccaggcagca aggcaataat tggctcatct aaaggtaatc ccaagaaagt tctgctcgag
      661 tgtttatcct tggcagaagc tatctttttt actaaaggat gtcctacaaa actgacagga
      721 acacctgcat tttcatagat agtcttttca aatgggaaga tgacagccat tttatcgatg
      781 ctatctttaa taagatggat acgtttgcct ttccatgccc atatttgagg gctgatgtaa
      841 taaataattt tgagccccag ttttttcttt gcgtacttgg ccaatctcaa attgaaagca
      901 gggtaatcta ccaaaatcag caaatcaggt ttttgtgtgc tcagatgctg tttgatatct
      961 tgaaaggctt tatggaatat tttcaggaaa ggaattattt cagtcaaacc ggttacagca
     1021 taacgcgtta aatctgaaat taaatgaacc ccggcggctc ttaagtgctt tccgccaatg
     1081 ccactaattt ccaggttcgg ataaactgct tttaattgct ttaccagctc ggcagcatgg
     1141 tggtcaccag attcttcgcc tgccactatg acaacacgac tagcattcgg catagcgttc
     1201 cacaaggtta ttattaatta aagcggtgat ttgttcggct gttttcaaag catcccttcc
     1261 ttcttcacct gtaacaagcg gcacgctatc attttctatg caattcaaga aagctctgat
     1321 ttcctctagt aatgcatctc ctttttcaaa ctccctttga tctctggtga tatcaggtat
     1381 tccagggaac atttctcccg tgcctttttt aaatacggca agctgtttgt tttggtaatc
     1441 aattgaaata taagaattct cctgaaagat tcttgttttt cgttccgttt tgaaactgat
     1501 tctgctggct gttacatttg ccacgcagtg gttagcaaag cttatgcgtg cattagctat
     1561 atcaattgct tttgttaata ccggtgctcc ttgagccgta atggatttaa tagggctctg
     1621 aactatattt tgaataatgt ctatgtcgtg aatcatcaaa tcaagtatca cgtttacatc
     1681 agttccacgg gggttaaaag gtgcgagtct ctgcgattct ataaataatg gtaattccag
     1741 atattcatcc aaggcaaggc gagcagaatt gaagcgttct aaatggccta cttgtaactt
     1801 cacctgatgt tgttttgcaa gtcgaatcag ttcctcagcc tgggatactg tttcagtaat
     1861 cggtttttca atcagaacat gtatcccatg ttgtaaacag aattttgcaa tttcaaaatg
     1921 cttgttggta gtcgctgcga tactgacagc gtctactttg ccaaacaggt catggtaatt
     1981 gatgtatgcc ggaacattta actcactgga caccgcttcg cagactgtag agtttacatc
     2041 acaaactgca actaactccg cattaggaat tagttgatat ttttgggcat ggaacctgcc
     2101 aagataacca acaccaataa ctgcacaacg aattttattc atctcatcat caattgataa
     2161 ttatttgtac ttatgatcgc atttccttag tgataaatca attgtatttc ctatgttatc
     2221 atgccaggta tacagtaatt gaggcattta aaactccaat caaactccag agattggttc
     2281 aaatcaaaga agtcgattct tgtcttgcac ttgcattcaa tagctattat cttacgccta
     2341 atttttcgca gggagcagtt aaatgaatac tgagctaaaa atattgatgg ctggggtcga
     2401 tgaagtgggg agaggaccac ttgcaggtgc cgttgtgacc gcagccgtta ttttaaaaaa
     2461 accaatagat gggcttaccg actcaaaaaa attaagtcca aaacaaagaa atttattagc
     2521 aattagaata aaggaagaag cgttagcttt tgcctatggg cgagcagaag ttgaggaaat
     2581 agatcaatta aatattcatc atgctactct attagctatg cgaagggcag ttgaagcttt
     2641 acccattcag ccagataatg tcgttgtcga tggagctttt actcctcaat tgaacatacc
     2701 atgcaaggct attgtccagg gagatagctt gatacctgaa attagtgcgg catccatcct
     2761 cgccaaggta ttgcgtgatg aagaaatggt agctcttgat aaaatatatc caggatatgg
     2821 ttttgcagaa cataaaggtt atgcaacccc tgtccataaa gaagcgctaa tgcgtttggg
     2881 tccttgtaaa atacatagaa gaagttactc ccctgttgcg gatttgattt caaaataaaa
     2941 ctctgagtgg tattaatttg gaaaaatacc gcccaaataa aaatacataa gaaatagctt
     3001 ttaaaatgtc atcatatgat ttatgcaaat cagagcaaaa aaattaaatc aattcaagct
     3061 attaaataat atacggtgtg agcttatcga cattaaaatt ccaaagcttg ccaggaaagt
     3121 taccatagct gttccaccat aactgacgag cggaagaggg atacctacaa ctggaataat
     3181 ccccatgacc atgccaatat tgacaaatgc agaaaggaaa aaggtcatgg ccagactggc
     3241 tgaaagcagg cgagtgtatg tagtttgtgc attattggca atatttaaac tgcgtaagga
     3301 aattaagact atcagcgcaa caatagcaaa tccgccggca aatccaaatt cttcgccact
     3361 taccgcaaaa ataaaatcag tagcatgttc tggcaagaaa ttgaggtgtg attggctacc
     3421 ttttaaccat cctttaccca ttagtccgcc tgagccaatg gcaattttgg attgaataat
     3481 gtgatagcca gcaccaagtg gatcttgctc aggatcaatc agggtataca ccctttgttt
     3541 ctggtagtca tgcatcagat tccagacaac aggaattgct gaacacatca gtaatgcaat
     3601 taataatatg attttaaaac gtattcctgc caaaaatacg acacataaac cggcaacagt
     3661 aaccataatt gcagtaccta gatcaggttg ctttgcaata agaagagccg gaataaaaat
     3721 aatcagggaa gcaataccaa ttgaacgtat gctgcttgga tgagattgtc ggtcgaaaaa
     3781 ccaggcggcc atcataggta cagccagttt cataatctcg gaaggttgaa aacgaaataa
     3841 ccccagctct aaccagcgct gagccccttt accaatttta cccattagca tgactgcaat
     3901 taacaagctt aacccaaccc cgtaaatcca tggcgtccag attttatatt tatgtggcgg
     3961 gataaatccc agtacaaaca tgattagaaa agcgaagagc agcctcatgg attggcgcat
     4021 gatcatgccc atattggcat ttgaagcgct ataaagtatc aagagtccga aggcaataag
     4081 agtaagtatt aatccaagta atggaaaatc cagatgaagg gactttgctg taaaacgata
     4141 tacgggctta gtcgtttgtc tactcatggt gttttcttaa taggataaag ttgataataa
     4201 gtgtcaagta ctttacgtgc tacagtggaa gcgatagtat cattttcaac taccacggca
     4261 atggcaattt ctggtttttc taccggagta aatgcaataa ataaagaatt atcccttaaa
     4321 tgctcaggta tgtcttcgta ttttgccttt tcatattgtc ttccgctgta tacttgtgct
     4381 gtacctgttt ttcctgctac cgggtaggga ggatttctgc caaaacggta acctgttcct
     4441 tcattgctgg ttagtacgct atgcatcgca tcaatcacga catcccagtt tgcttcatct
     4501 ctcagggaaa ccgggtattc ttcaaaaggc ttgtattctt ccgtttcatt ggtatcgctg
     4561 tttactgttt ttatcagtag gtgaggtctg aatcgttgtc catgctggct tagggaagct
     4621 gtggcattag ccatttgcaa aggggttgcc agcataaatc cttgtcctat tgcagaaatg
     4681 agagtatctc ctggatacca ggatactcct ttagtttgtc ttttccagcg agcgctaggt
     4741 attactccat ttgcttcttc atgaaggtca acgtgtgtta gttgtcccaa gccaaatttt
     4801 acaagcatgt cttcaatgtt ggagatcccc attttattac ctaactggta aaagaaagtg
     4861 tcacaagaga ctgtaatagc tcgtttgaaa ttaataaccc catgacctgt ttttttccaa
     4921 tcacggtaaa catggctgga agtagggagt ttgtacttac cggggtcata tatttcagtg
     4981 gtcgtggtaa tgaagccttt atccaaacca gccagtccaa caaacggttt gattgttgat
     5041 gcaggggggt aaacacctcg aactgctcga ttaaaaagag ggcgctggag ggcattggat
     5101 aacactttat aatccgcttt actgactcca ctgacgaaaa tattgggatc aaaacttgga
     5161 gaactcacca tcgcaagtac ttcgccattc cgagagttta tgactactac cgcacctcgt
     5221 ttatccttca atgcattata tgccgcttcc tgaagtctgg cgtcgatgct taaatacagt
     5281 tttgctcctg aatgaggatt gactttattt attattctta aagtgcggcc actaacatca
     5341 gtttctacca tttgataacc cactttacca tggagaatat cttcatagta tttttcaaca
     5401 ccggccttgc caataaagtt ggtagcgcga taattagtgg agtccacttg ttttagttcc
     5461 tgaatattaa tgcgaccgac ataccccaag gcgtgggcag taatctctcc aagagggtaa
     5521 tgacgcatca atctcgcttt aatgcttact cccgggaaat gatactgatt gctggcaaag
     5581 gtagctactt cttcctggct taattttaat ttgataggaa tgggaacaaa agaacgattt
     5641 tgtaatctgg tcttattgaa actatcaata tcttcgtctg taattgaagg aattaactct
     5701 cgaagtttag ttaaagtctg tttcatgtct ttaacacgtt cgggagtgat ttccagcaca
     5761 tacactggta tattttcagc gagtaatact ccatttctat ccaaaataac ccctcttgga
     5821 ggagcaatcg gtatcacgct catttgattt tttaaagata aggtctgata tcgtttaaat
     5881 tctgatattt gtaggaaggc caatcttaaa atcagtatta aagataaaat aatcagaatt
     5941 gcaattaata gattaagtct aaatcgttga tgttgggatt cagcacgata gtttttaaat
     6001 gattgattcc gatacatcgc agaaaatgtt gtatgacaaa acaaaagtag gaatagtaac
     6061 tgaaattaca gatttttacc aattataaaa tacttactct gtatgttgtt tcggtacgat
     6121 ctcgcattaa tgtgctattg tctagattac tctaccgcgt agcatgctcc gcttgaattc
     6181 ataaaattct tgatatttac aatcaataaa agtatagtga ttgaccaata tgtataaagt
     6241 tagtgaaaaa atgaaattta cgcaagataa atttgagaag tttgatgaat taaccaaaga
     6301 tctccaaatc gaaacagcac aaaatttatt aaatcgtggt ttagtaaact tgacccagac
     6361 atcaaagcct catttagttt tatttaaacc catggttgat gttcgtaagt tactgtactg
     6421 cgtagtatgt ggtgagcatg ctaaggtgca atcaatgtta gagaaagact gtagtttaat
     6481 tttcaaaaga ggtaaagtca cagattgctc tggacgagaa tttgagagta tcagtgcttt
     6541 tgaatatgta ctgtgggcct tggataaaca catgtgggca gcaatgattg cgtgcatacc
     6601 aagtaacgaa gaaagcaggg gagtattcgt acaattgctt gctcaataca ataaagttaa
     6661 cacagacgga gtcacttata gacttaatgg gaacaccata actgaacagc attttgattt
     6721 cgaaaatacc atcattaaag agcttaaaac tcaagtaaaa gagatgaaaa agcaagtaga
     6781 ttcggtaaac gcccacaatc cgactggaga tgccattgat aattgggatg ccattgataa
     6841 tcagtggaga gaaggtgttg gtggtgcaca aaaactgctg ccgatgcata ttgttgatga
     6901 atactgctct aatgaacctt tttatcctgt gcctcaattt acctcacagc caaaatcatc
     6961 gaaacaattc tacaattgga ctactaaaaa ggatgaagat tggtttaacc tgaattctaa
     7021 gatgggggtt gatttagcta tatataaagg tcgcggtgat cgtaccctgg ggtgcgggca
     7081 tgtaggcatt gcggcatctc gtgatgattt gggagctata tcggcattat gtgaagtaag
     7141 aacaatggac tttattaagc tcaaatcgct acttgaagag cgcgctacac tggataatta
     7201 tcaccaagtt gttccaatgt gatcgagttg acgacatgtc tatgaagaag cctggcggtg
     7261 taagtcgcca tatgttgatg tgagtatcaa tctaaagtaa gagaattgat agcaaccaaa
     7321 gatcgttggg aatagttttg gggcgtatta atcaagcagg attaattggt ttggcggaaa
     7381 tttactggtt atttagaaat aaatgtatac taattcgaga ccacaccctg ttattttcgg
     7441 attagagttc atgcatgagg aacttttgaa acgactatat atccttattt atgataagga
     7501 tggttgttaa tgatagacca tgctctatag agggattcca gtaaaataat gcgaaccaag
     7561 ggatgaggaa gggttaattt ggataacgac cagcgttcat cgcaaagagt tagtatttca
     7621 ttggataatc cttctggccc gccaattata aaacaaaggt gacttgaaat ttgttgtaat
     7681 tgcgtcaact taagtgctaa ctcctcgctg ctaaaggatt ttcccagcat gtccagggcg
     7741 ataaggcgag cattggcagg aagggcatct ttaatcaaag cactttcttt ttccagtatt
     7801 cttgctaaat ccgaggattt attacgacgc agcaaaggga tttcgatgag ttttatctgg
     7861 atgccatcat gaaacctctt tgcgtattca ttaactcctg agttaaccca gtcgggcatt
     7921 ttattgccaa gagtgataat ggtaattttt agcatacata atgtcaggag ttacctgggt
     7981 gttcttccca taatccttca agattataaa aatgtcggaa ttctggttgc atgacatgca
     8041 atatgatgtc tccaaaatca attaaagccc aatcaccatg ttctattcca gtagaactca
     8101 agcaaggcaa accagcaacc ttcatatctt ccagaacttt ttgagctatc gatttgacat
     8161 gtctggatga acggcctgat gtgacgacca taaaatcagt gattgttgtt tgtttatgca
     8221 catcaatgat tttaatatct atcgcttgaa tgtcttcaag agattttaac agtttttcga
     8281 gcgtaggatt tttttcaagc ataactaaga tatattccgt caaaagggtt gaataatatc
     8341 aacaattgat ttgtataaaa agggttttag ttttcaagtc tggaaaacta aaacgtaaaa
     8401 atctaattat tgtttttcgc atatctgaat atgagtcaat aaaaatttaa tcacagcaaa
     8461 caataccagg cagcctaacg tagcaatgaa taatatactg agttgttggg tgaatgctgt
     8521 agcgtagagc gtttttaaaa cgattatatt tgtctctcca ttaggaatgg cagtcagatc
     8581 ggctaatttt cctgaaagga atcctcctat tcctaatgaa acgaaaaaga tacccatcat
     8641 ggtgcttacc ttgtttttgt cagcaagtac agttattgct gataacccta ctggtgaaag
     8701 cagtaattca gccaaagaaa tcatcaggta ggcagggatg ataagaagag gtgaaatcaa
     8761 aatggattta tcaactacag tacttacaaa ggcaataatt gagtaggcga gtgtcatgaa
     8821 taccatagca agtacaaatt ttttcccggt acttaagcct ctttcaatca ttgtaagact
     8881 atggtgcttt ttggcaagaa aataaccaat tattaacatg ccaatacttt gcactgtgat
     8941 gtagtatgga gggggaaaat cgatacctaa aaagcttggc tcgactactc gcgcaataaa
     9001 taatgttaaa gacatgaaca tctgaaaata aaatgcccag aaaataacag agatgatgca
     9061 caataacccg ataactaatg tttgcctgga ttgatttgca ctttcacgat ttactgaata
     9121 taaaatgtaa ctggctgaaa aaagaaccac aagtccaaac atgatattcg ccagctgagg
     9181 tgaatttaaa atataaaatg acaatgacca cagaaaaacc aataacacaa aagctgaaat
     9241 gattttacct ggttggaaaa caaaaggatt ataatcctta attttgtact gatgtattcc
     9301 aaataaaaac actacaaatg cgatgattag cccgattgat gcactggtga aggaagcaga
     9361 ccagccaaaa tactcactaa acttgcttgg caatgttgtt ccgaggatta ttcctgtcgt
     9421 gatgcccata taaaaaatag taaaaccact ttctcttttt gttgagccaa ctggatattc
     9481 attacctagt aatgaagaaa tgttaggttt taataaacct gttcctacag ctactcctgc
     9541 aagtgaagag gttaatgcca gagaattatc aatcaatgac aacatgcaat aacttaaaaa
     9601 cagaacaata gcacccagca atatagcgcg tttttggcct attaatttat cagcaatcca
     9661 gcctcctact aatggtgaga ggtaggtgag tgccgtgaac gatcctacta aagaatagat
     9721 ttctttgtcc ggccatttaa agtgtaaggc caaataaagt gctaataagg attgaactgc
     9781 gtaaaagcca taacgttccc acatttcggt tgtaaagtag atgcggagag attttgggtg
     9841 tttttccata ttatttaagt aactacgatt aaattaattt attaaataaa aattatatca
     9901 tataaaaaac agtatgaata ttgattggtc attttatctg gtttttctca actcattaga
     9961 ttatctcatt cccggaggta gttgactggt gtatgatgct aaaaaatttt ttaatcgcta
    10021 tttgatagga aataataact gaagttttca tagcaataag gagtattctt gatgagcaag
    10081 gacaagaata aacgtgttat ttctgcccct agaggtaccg aaattcaagc taaaaattgg
    10141 ttaactgagg cggcattaag gatgatttgc aataatttgg atcccaatgt tgcagaggat
    10201 cccgattctt tgattgttta tggtggattg ggcaaggcag ccagaaactg ggaatgtttt
    10261 gacgaaattg ttcatgtatt aaaattatta aataatgatc agactttact cattcagtcc
    10321 ggaaaacctg tcggtgtatt tactacccat gaagaggctc ccagaatact aattgccaat
    10381 tctaatttgg taccgcgctg ggctacttgg gagcatttta acgaactgga taagaaaggc
    10441 ctgatgatgt atggtcagat gaccgccggc agctggattt atattggttc tcaaggcatt
    10501 gtgcaaggaa cttatgagac atttgttgcg gcagctaaaa aacattatca gggtgatttg
    10561 tccggacgtt ggattttaac agcgggttta gggggaatgg gtggagctca acctttagct
    10621 gggactatgg ctggtgccag cgttctggct gtagagtgtg acaggcaaag gattgagaaa
    10681 cgtttacaga caaagtatct ggatcggtac actgataatt taagtgaagc attagactgg
    10741 attaatgaat cctgtcgccg taaaaagcca gtttcagtag ctgttttggg aaatgctgcc
    10801 gaaatatttc ctcaactggt taagttggga gttcagcctt ccctggttac cgatcaaacc
    10861 agcgctcatg atccgctcaa tggctatttg ccattaggat ggacattgga acgagctgtt
    10921 gaaatgagaa aaaaatcacc agaagaggtt gttgatgcag ccaagaaatc gatggctgtt
    10981 caagtgcatg caatgcttga gtttcataac aaaggcattc ctgtatttga ttatggcaat
    11041 aacattagac aaatggcatt tgaagcggga gaaaaaaacg ctttttcctt tgagggattt
    11101 gtaccggctt atatcaggcc attattttgc gaaggaatcg gaccatttcg atgggttgca
    11161 ttgtcaggtg atccagaaga tatttatgca acagatgagc gagtaaagca gctaattcct
    11221 gatgatcctc acttacatca ttggttagat atggccaggg aaaaaatttc atttcaggga
    11281 ttaccagcaa ggatatgttg ggtagggtta aaggatagag cgcgccttgc cctggctttc
    11341 aatgaaatgg ttaaaaataa acaagttaaa gcgcctattg tgattggccg tgaccatttg
    11401 gattctggtt ctgtggccag ccccaaccgc gaaacagagg gtatgctgga tggcagtgat
    11461 gctgtttctg attggccttt gcttaatgcg ctgttaaatt gtgccagcgg ggctacttgg
    11521 gtaagcattc atcatggggg aggggttggt atgggctttt ctcaacatgc tggcattgtg
    11581 attgttgctg atggaacaga aaaagcggct aaacgattgg ctcgcgtgct acacaatgat
    11641 cctgcgacag gggtaatgag gcatgctgat gcaggttatc aaattgccaa ggaatgtgcc
    11701 aaagagaatt cgctttggtt acccatggaa tcttaaaagg agcaggttta tgtcagaaca
    11761 ttttatattg cagcctggtc agttatcgct agtttctatt aaacaaattt tagatgagga
    11821 actatcttgc gttcttgctg agaacgcctt tgaattaatt agagcttctc accaaacggt
    11881 taaaaaagta attgatgaaa agaaaacagt atatggaatc aatacgggtt ttggttctct
    11941 ggcaaaccag accatttctt cagattgttt gaaagagttg cagcgtaata ttgtactttc
    12001 tcatgcttgt ggaacgggta aattattgcc ggatagcgtg gttgccttga ttttgctttt
    12061 gaaaattaat aatttgtctc aagggtattc cggagttagg ctggaattaa tcaatgcatt
    12121 gattgctttg tttaatcata aagtatatcc atgtattccc tctaagggtt cggtaggtgc
    12181 gtccggagat ttagtcccgc ttgcgcattt gtctttacct ttgttaggag aaggcgaagt
    12241 aaggcatcaa ggtcaggtga tcagtgctga ggaaggacta aaactggcgg gattgaaacc
    12301 gcttgaattg gaagctaaag aaggattagc actgcttaat ggcttacagg tatctaccgc
    12361 tttagctttg tcagctctgt ttattagtga gacattgttc gaaacagcga ttatttccgg
    12421 cagtttgtca gtagatgctg ccagtgggag tgatgtgccc tttgatgaca gaatacatca
    12481 aattcgaggg catcaagctc aaatttctgc tgccagcatg tacagaaatt tattggctgg
    12541 tagtcaaatt cgagagtcac acaggcattg taatcgtgtt caggaccctt attctttaag
    12601 atgtcagcca caaatcatgg gtgcagttct tcatcagatg cagtttgttg gacaaactct
    12661 ccaagtagag gccaatgcaa tttccgataa tcctctcgtt tttgcagagc agggggatat
    12721 tttgtctggg ggaaattttc atggtgaaat catcgcaatg gctgctgaca atttagcatt
    12781 ggcattatcc gagataggag gtagtgcaga acggcgaatc gctttattaa ttgataagaa
    12841 ttttagtgga ttgcctgctt tcctggttag agaaagcggc ttaaattcag gttttatgat
    12901 tgcccatgtt actgctgcct cctgtgccag tgataacaaa gcgcttgctc atccgcactc
    12961 cgttgatagt ttgcctacct cggcaaatca ggaagatcat gtttctatgg caactagcgc
    13021 agcacgtcga ctgcatgaaa tgatcgataa tacttcaaca attcttgcta ttgagttact
    13081 ggcagcttgt caagggctag agtttcataa acctctaaag acatcaccgc aattggataa
    13141 aatttatcaa agcgtaagag gcgtggttaa agagtatgat aaagatcgct attttgcacc
    13201 tgatatagaa aaaattaaaa agaagatttt agataaagaa ttctcattac tcacactcac
    13261 gaacgaatag caggaaaaat acaatggaat tgatggcaga atttctaact ttgcatcaac
    13321 aacacaaaaa caatccttat ccttctttgt ctgaaagaaa aaaattgctg attgcaataa
    13381 aaaaaatctt gcagacagaa gcttacactt tagcagaagc aataaataag gactttactc
    13441 atcgccctgt tgaggaaacc ttatttttgg aagtttttcc aacgataaaa gccattaatt
    13501 tttgtttaaa aaatatgaag aaatggatga gaaagagacg aaggaatgtg tcttggttgt
    13561 ttattcctgc acaagcctat gttattcccc aaccccttgg agttgtaggc atcctggttc
    13621 cctggaatta tcctgtctat ctggctttag tccctgctat ttatgccttg gcatcgggaa
    13681 acagggtaat gataaaaatg tccgagttat ctccacatat aggcgatact ttacaaaaat
    13741 tggtacatgc gagtggaata aaccattcta tcagcgtcat taacggcgat attgagctat
    13801 caaaacactt tgcttctctg ccttttggcc atcttatgtt cactggctca actaaggtag
    13861 gaaaactggt gatgaaggct gccagcgata acctgacccc agtgacactg gagctaggtg
    13921 gtaaatctcc ggcaatatta tctcccacca tgaatccagc ttattttaag cgcctgttta
    13981 tggggaaatt attgaatgcc gctcaaactt gtattgcacc tgattatgta ttgattccta
    14041 aaggatggga ggagagagtt gaaacagagt ttaagaagtt tattaatacc ttttatccag
    14101 atttaatgag taatgagcag tattccagta tcatatcaga gagccataaa aagcgattac
    14161 ttgatctggt cgaagatgcg cgaagcaaag gggcccgtgt tgttgagttt ggccatttaa
    14221 ttccgaatag ttcgaagcta cctgtgtttt tattatttga tataacaaat gatatgcttg
    14281 ttatgaagga ggaaattttt ggccctgttc ttcctgtgtt gacttataat tcgatatttg
    14341 atgccgtaga tttaatcaat tcttttccta atccgcttgc gctatattat tttggggaag
    14401 ataagaatga aataaagatc atacaaacaa caacattatc tggtgctttg acaattaatg
    14461 aaaccttaat gcatattgca attgatgatt tgccatttgg cggtgttggc catagtggta
    14521 tggggcacta tcatggaaaa gaagggtttg ataccttttc aaaattaaaa ccggtattag
    14581 tgcagcgatt tatatctaca gtgagctggt tatacccacc ctatggtgct tttatgcgta
    14641 tgtttttggc ttgggttggt ggcatcaagt agaaggagaa atcatgaata aagtcatttt
    14701 tattacgggt ggcagcagag gaattggtag agaaatcgct ttaaagtttg cagcaaaaga
    14761 taaagctaat atcgttattg cagcgaaaac agcagaacct catcctaaat tggaaggaac
    14821 tatttattca gttgcaaaag aaattgaaga gttaggcgga caggccctgc ctttaatggt
    14881 tgatgtcaga gatgagcaac agatccaaaa tgcgatcact aaaaccatag aaacttttgg
    14941 aagattggat gtattagtca ataacgcgag cgcaatcaat ttgactgata cattgaatac
    15001 tccaatgagg cgttatgatc tcatgcagag tgttaatgtc agggcaacgt ttgcatgttc
    15061 tcaagcagcc attccatatc ttttaaaaag cgagaatccg catattctga ctttgtcccc
    15121 acctttaaat atggataaaa cctggtttgc accacatctg gcttatacca tcagtaaata
    15181 cggtatgagc atgtgcacat taggcctcgc tgaggaattt aaagaggcgg gaattgctgt
    15241 taattcttta tggccgaaaa ctactatagc tactgatgcc ataagagttc attttcccag
    15301 tgagctgtat atggcaagca gaaagccact cattatggct gatgctgctt attggattat
    15361 caaccaacca tcaaaatcaa tcactggaaa tttttttatt gatgaagacg tattaaaaaa
    15421 tagtggtgtc actgattttt cttgctatgc tatgaatcct aaagctcaac tttgtcctga
    15481 tttattttta tgaaggtaga aatctatact gatggtgcat gtaagggaaa tccagggcct
    15541 ggcggctggg gcgtattgct ccgatataat ggtagagaaa aaactcttca tggaggagag
    15601 gcgcaaacta ccaataatcg catggaatta atggcagcaa ttaagggcct ggaagcatta
    15661 aaacgtcctt gtgaggtgga tttgtatacc gactcccaat atttgcagca aggaatgaag
    15721 gaatggatca aaacatggaa aagaaatggt tggcgtaact ccaagaaaga gttggttaaa
    15781 aatgcggaat tatggaaatc attagataat ctggcttcta ttcataatat tcattggcat
    15841 tgggttaaag ggcactcagg ccatttggaa aatgatttgg ttgatgcttt ggcaaacttg
    15901 ggtattgagg aattatcata gagttatgag acaagttata ttagataccg aaacgactgg
    15961 tattggacct gaacagggac atagagtaat tgaaataggt tgtattgagc ttatcgatag
    16021 aaaattaacc ggcaagcact tccacatata ccttaatcca cagcgtgaag tggatgaagg
    16081 ggcatttaga gttcatggta taagtactga atttttgcaa gataagcctt tattccagga
    16141 tattgtgact gaatttgtac aatttgtgga aggttcagaa ttaattattc acaatgctcc
    16201 atttgatgtt ggatttctga attctgaatt aaaacatgtg aaatggaata agacattaga
    16261 agattattgc cggattcttg atactttgat tttagccaga gaaaaacatc caggacaacg
    16321 taatagtttg gatgctttgt gtaaacgtta tgaaatagat cattttaatc gtgaactgca
    16381 cggtgctttg cttgatgccg aaatattagc ttatgtgtat ttggcaatga caggtggcca
    16441 aagcagttta tttactgagg ttgaatccag tttgagtcaa tcaaaaataa aaactcaaga
    16501 gataaagtca ttaagtttaa aaaaaccagt aataatgaag gctaatgaag aggaattaat
    16561 tcaacatcaa catttcgttg aatttatatc taaaaaatcc ggtatcaatc attgggaaga
    16621 ggtatagctc tcatgattat ataaattata aataattatt ctgttacgct tattattcgg
    16681 tttcgcgtct gccaatacta aaccttatac aataacaaaa ttcttcagac atcttattcc
    16741 tggtcgctat cctcaaaaca agcaacccaa gtgattggtt ataggcagag gcgaagccta
    16801 atgattatct caatcaagca atttttttat tatctatttg ggaattagat gtttttcagc
    16861 aaaatctttt ccgatacatt ctcctaatga tcttccccat gtgccagctg tttcactatt
    16921 gattttatct ggcatgctgg catacagttc atcttggcat tttttggtgc tttcaggaat
    16981 taaagtcacg cattgttcat aagtcatttt aatttcatca aatcgttttt tgagatccgg
    17041 atcttgaata aatcctttgc agataagatc tggtaataag ggagtcatag agtttaacca
    17101 agcatcctta gtcatctcat taggatttgc tgtgtcttca gcattggata ttaagggaaa
    17161 taatgaagtg cttagcacga gcgtggtagc aataagtaat ttccttgaca tgatttattg
    17221 tccttatttt tagagtctca tatatatagt aggacaaaaa atacaatttt tcctttattt
    17281 agtaatttat agttacttga aactaaatga ttgattcctc tttacttctt cttgccaaca
    17341 cacctatgtg cttgttgtac atcttagaca atcatggctc catggcttgt tgggtaagtt
    17401 atttaataat tgattagccg tggcataaag gtgatgaaat atttgaattg ctcaaaaatt
    17461 aaactaaaat aataaagtga cggaaaaaaa ataggttaaa tcatggaaaa gtccaatttt
    17521 cgcttaaatt ttttgtctca aattttgctc ttcactttag gggtattttt attctgtaat
    17581 gggtacgcag cagggaatac ctttattttt aatcccaata cattaacctg gaaagctatc
    17641 actgctaatg gaaaggtgat acgtacaggg cgcggatcag gtggaaaaca ctactgttct
    17701 gatgttcgca gatcatgtaa aacaccgtct ggcgttttta gagtgattag caaaggtggt
    17761 ccaggatgtc ggtccagtcg ttatccagtg ggtaaaggtg gagcaccaat gccttactgc
    17821 atgtttttta gcaaattata tgcaatacat ggttctccgg atgtacccaa ttataacgcg
    17881 agtcatggtt gtgtcagagt gaagcctcag gatgcaagat ggctaagtca aaattttatt
    17941 aaaattggaa cgaaagtaat tattaaatcc tattaatagc attaattaat gcatcataat
    18001 tggtggttat gtagtataat tggaattatt
//
LOCUS       DASLOE010000045        17968 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.045, whole genome shotgun sequence.
ACCESSION   DASLOE010000045 DASLOE010000000
VERSION     DASLOE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17968
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.045"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            191..736
                     /locus_tag="U6309_001269"
     CDS             191..736
                     /locus_tag="U6309_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEN5504718.1"
                     /translation="MKLLFDFFPIVLFFIVYKFFGIYTATAVAMVASLTQVAFYRLKF
                     QHYEKMHLFSLAIIMVLGGATLFFQNPWFIKWKPTGIYWLSALVFYGSGYIGSKPLIQ
                     KMMEANINLTTKIWYRLNLAWTLFFIVMGALNLYVAYHYDTDVWVNFKLFGGVGFTLL
                     FVLIQAFYLTKHTDEKSFEKQ"
     gene            797..1474
                     /locus_tag="U6309_001270"
     CDS             797..1474
                     /locus_tag="U6309_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN5504719.1"
                     /translation="MRLLLVEDDELLGDAVKAGLTQFGYVVDWLKDGEAARAALKSES
                     FELIILDLGLPKLSGLSLLQNIRHDGNSTPVIILTARESIEDRVKGLDSGADDYLIKP
                     FDLNELSARIRALVRRSQGRADSVLQYKNITLDPAAHSVFVDDVLVNVPRREFALLQK
                     LLENSGQVLSREQLMQSIYGWEEDVDSNALEVHIHNLRKKLNANFIRTIRGVGYMAEK
                     NDSVLLS"
     gene            1471..2886
                     /locus_tag="U6309_001271"
     CDS             1471..2886
                     /locus_tag="U6309_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN5504720.1"
                     /translation="MKSSIRKFLLINLLLAITITTTLTAIGNYYLDQKDIQDHLDTLM
                     AISALSYQALLGDDLHQRPLAKIQNALESIPQKINTYYQKRYLNDEPQENYLDKFNFQ
                     VWTNGGKLLLHSPTAPKIPLTAETDGFSDKRISNQDWRVFTTYNDKAGIRTVLAERYD
                     TRNELGHRIAQDDLYIMLLTFPLSGLLIWIIIGRGLDSLDRVAEEVANRAPSHLEPVD
                     LQEVPEEIKPVIDELNKLFFRLKEGFEREKRFAADAAHELRTPLAALKAQAQVALNSN
                     DIEEKNQALQKLIASVNRSTHIVQQLLTMSKLVPEATELHEQDEVNLSKLTREILAML
                     APSAVEKQIELEFETDEKMPKIYGNPTALGILIRNLVDNSIRYSMENGRVIVRLLKHN
                     NEIILEVCDNGPGIPPELQKRVFERFFRVLGNKSPGSGLGLAIVQQICELHGGRVVLD
                     SPKIGTGLIVRVYLPIKTKPK"
     gene            complement(2927..3247)
                     /locus_tag="U6309_001272"
     CDS             complement(2927..3247)
                     /locus_tag="U6309_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504721.1"
                     /translation="MKRMIGFMVSVVLTFAVNAADSQVNTHKHQTDKGKVTPGMSIQR
                     NTEQSTAITADAASSNTTEKVTSQQMKEHKNSVKEANYNAQKKEEEAKTATDTQKHEA
                     DSPS"
     gene            complement(3299..3898)
                     /gene="ruvA"
                     /locus_tag="U6309_001273"
     CDS             complement(3299..3898)
                     /gene="ruvA"
                     /locus_tag="U6309_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEN5504722.1"
                     /translation="MIGWLHGQIIDKHQPGKLVLDVNGVGYDVETSLNTFFQIENGNQ
                     PIGLHIHTIVREDALLLYGFLDKEERSLFRSLIKVNGVGPKLAMTVLSSISPKEFIQC
                     IHQENAALLTKLPGIGKKTAERLVVEMRDSIKQFDGSVSDTFQKQAGSTHSQQEAISA
                     LEALGYKPQEAWKVVNKIDNGNKSCEQLIREALQILSSR"
     gene            complement(3895..4419)
                     /gene="ruvC"
                     /locus_tag="U6309_001274"
     CDS             complement(3895..4419)
                     /gene="ruvC"
                     /locus_tag="U6309_001274"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEN5504723.1"
                     /translation="MTIILGIDPGSRVTGYGLIKESDRKITYIDSGCIRTSNDAELSH
                     KLLQIYDGICELMDHYSPTEVAIEQIFMHNNPNSALKLGHARGVAMVAAASHRAKICE
                     YSAREIKQSIVGYGAAEKDQVSHMVVELLQLNRAPQKDAADALAIAICHSHMRNGLSK
                     LIGSRGTKRRRMRL"
     gene            complement(4419..5162)
                     /locus_tag="U6309_001275"
     CDS             complement(4419..5162)
                     /locus_tag="U6309_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN5504724.1"
                     /translation="MAGHSKWANIKFRKGVQDAKRGKIFTKLIREITVAARMGGGDES
                     SNPRLRDAVKKALNANMKRDTIDNAVKRGVGGVDGEAMIAMRYEGYGPGGVAILVDCL
                     SDNKNRTVSEVRHAFSKHGGNLGTDGSVSYLFTNQGEILMASNQPEDKVMEIAIDAGA
                     SDVAVEDSQIEIITPVEAYHTVLNALQDAGLEVEQSHLTMRAQTLIPISDETAESLIK
                     LIDMLEDLDDVQEVYSNAEFSEKILESMN"
     gene            5309..6559
                     /locus_tag="U6309_001276"
     CDS             5309..6559
                     /locus_tag="U6309_001276"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEN5504725.1"
                     /translation="MYLQGFGNYHHSEAVKGALPPNQNSPQHCSLGLYAEQLSGTSFT
                     RPRHNNLRSWLYRILPTVTQGTYYPYEFNIMQPFVDELSPNAMRWSPLYNSSQIKCDF
                     VEGLFHIAGSPLVNTYTYYCNRSMSDKYFANNDGELLFVPYAGEIHLHTEFGKLMLSS
                     GSIAVIPRGVKFKVEVISKEAKGYLCENSGNPLTLPQLGPIGANGLANPRHFQYPVAA
                     FENSGGEHTIICKNQKKLWFTVCNHSPLNVVAWHGNYAPYCYDLSLFNTINTVSFDHP
                     DPSIFTVLTSESEIPGVSNLDFVIFPPRWMVAEHTFRPPYFHRNYMNELMGLVYGEYD
                     AKKEGFIPGGISIHNCMTPHGPDYESYEIAASQDLKPNYINSLAFMFETKDYWQVTEQ
                     AYRHPSRQMDYLNCWQGFKIEFSQ"
     gene            complement(6662..7687)
                     /gene="rpoS"
                     /locus_tag="U6309_001277"
     CDS             complement(6662..7687)
                     /gene="rpoS"
                     /locus_tag="U6309_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HEN5504726.1"
                     /translation="MQDEEEPIKDKEFKEEEWSEPDDDSLLSEEDIEPDIEKMEEELD
                     ELPEFTDDEAFPSFQRGKNIAKALDATQLYLGEIGFSPLLTAEEEIHYATLALKGDME
                     ARKKMIESNLRLVVKIARRYLNRGLPLLDLIEEGNLGLMKSVEKFDPKRGFRFSTYAT
                     WWIRQTIERAIMNQTRTIRLPIHVVKELNVYLRAARQLTQKLDHEPSPEEIAEMVDKP
                     LEDVQKLLGLNDKVASVDTPIGFDETKSLLDTIADENSLNPAELLTNENLRQHIESLL
                     DKLTENQQQVIARRFGLRGFEKATLEDVGKEIDLTRERVRQIQVEALKTLRTLLERVG
                     LTQEDLF"
     gene            complement(7772..8515)
                     /locus_tag="U6309_001278"
     CDS             complement(7772..8515)
                     /locus_tag="U6309_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN5504727.1"
                     /translation="MMRLFRKQLICIFFILSVAGCGTRSDLAPVTELKWQPYSKYQKF
                     HTVRSGETLYAIAFRYDTDYRRLAIINHLRPPYSLRVGQVLSLKGIVPKSNYLHSAPR
                     ANLHITHKPKQKIIYSPAYNVRSSSGWLWPVTGRVVTTFIPSQGKKGINIACNRGDKV
                     RASASGVVAYAGSGLSGYGNLIIIKHSNEYLTAYGNNARNLVTEGQRVSAGQVIAEAG
                     LIDRSYWGVHFEIRRAGVPVNPLNYLQKG"
     gene            complement(8534..9292)
                     /gene="surE"
                     /locus_tag="U6309_001279"
     CDS             complement(8534..9292)
                     /gene="surE"
                     /locus_tag="U6309_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEN5504728.1"
                     /translation="MKILVSNDDGVLAPGIKILANELSTLGEVKVVAPDRNRSGASNS
                     LTLTQPLRVKQLDNGYYSVDGTPTDCVHLALTGFLEPIADIVVSGINEGANLGDDVLY
                     SGTVAAAMEGRYLGLPAIAISMVGDNIQHYETAAIIAKQLVIKLSANKLPSQTILNVN
                     VPDLPLNQIRGLQVTRLGTRHSAEPIIKEYDPRGRPIYWVGPPGIEADAGAGTDFFAI
                     KTGHVSITPLHLDMTHYKLFDHLSNLLNEICIEN"
     gene            complement(9295..9678)
                     /locus_tag="U6309_001280"
     CDS             complement(9295..9678)
                     /locus_tag="U6309_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504729.1"
                     /translation="MNKYLVIILITLGLVSCQFKKDESYYRSHPSELQNALKLCPGTQ
                     PDGLSCQQLEAIGRRMNGLAYQLQYNPQEFGNKILALQQVIANQQREIESKKDNAELQ
                     DSLKRNQEDLAYYLAVVKWLESPES"
     gene            9758..11473
                     /locus_tag="U6309_001281"
     CDS             9758..11473
                     /locus_tag="U6309_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEN5504730.1"
                     /translation="MLDFSLNRDPQTGELYIETSLCGKPLLTTPQLNKGTAFTQEERK
                     DFGLLGKLPHRVETLDEQVKRAYLQYSSYTTRLQQHIYLNNLHDKNQIVFYKLLSRHL
                     GEMLPIIYTPIVGAAAKRFSHEYRQPRGLYIAHSDKNQLEEIINNRSNPDIDLVVVTD
                     GEGVLGIGDQGIGGMDIPVAKLMVYSLCGGIDPTRTLPIFLDVGTNNQELLNDPMYLG
                     CRHPRIKSSEYDDFIKTFVNEIHKQFPNAFLHWEDFGRGNARRILDQFQDELCTFNDD
                     IQGTGAVTLAAILAACDVTGLPLHEHRIVVFGAGSAGTGISDQIVDAMVKSGLSLTDA
                     YDRFWLIDKQGLLLATDQELTDAQKPYARNPIDIQSWEINNKQHPSFTDTIRHVKPTI
                     LIGCSAQTGAFSQDIVETMSTACERPIIFPLSNPDEKCEAQPSDILAWSKGRALIATG
                     TAFAPIEYQNRMVQIAQCNNALVFPGIGLGVLAVSASRLTKDMILAASQTLSKFAPSK
                     KDSFLPLLPSLDNAQIVAKEIAIAVAQCAIDSGYAQKNQDKDLPRLIDELFWEPRYLP
                     FRKRG"
     gene            complement(11530..11901)
                     /locus_tag="U6309_001282"
     CDS             complement(11530..11901)
                     /locus_tag="U6309_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEN5504731.1"
                     /translation="MKKLFSGLLMGFSALAYANNEVVINVSSKNPSFVVSLAANPTTG
                     YQWSVVEFDKNLLTLSNSIYEKPKTNLIGAGGKMLFTFNLNKGKTYPTQTKMVFKYAR
                     SWEPESGTIKNVTINFVNPSD"
     gene            complement(11938..12255)
                     /locus_tag="U6309_001283"
     CDS             complement(11938..12255)
                     /locus_tag="U6309_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HEN5504732.1"
                     /translation="MIIRNYLFAIAFLMIVYPNAYAMRCGDKLVYEGDSKYTVLSKCG
                     EPLDKLVYEENVPLYNAAGYQIGYGTNTVETWIYQKSPVDFQYELIFDYGKLKQINVN
                     RNP"
     gene            complement(12338..14149)
                     /locus_tag="U6309_001284"
     CDS             complement(12338..14149)
                     /locus_tag="U6309_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN5504733.1"
                     /translation="MNSYNKKSEELPKTLGRFFWHFIKKQPLAFTVFFVAPIAMVLEN
                     NAIPYSLKMIIDALGGHSGEENIFSAVAPALWLGGGAWFALIVILRLQNWWQGYVIPR
                     FQADVRMSVMEYLSRQSYHYFSNQMAGGLANKVNDLPRALDSIFNIITWYAIAAFASI
                     IVALILMCTINYWFAVILLSWIIFQLYISYRLSKKVDQYAKENAEDKSQLSGKIVDSL
                     SNSSVVKLFARSRDELAYISASQNMEVQSNKKLSIYMNIFRLYLDIPVTVMLVAMVYF
                     LLTFWNKKLITTGDLVFIFNVSFSIMTQIWYLCHAIAEFFREIGIARQAIALLSAPIE
                     VEDAPDAKPLIVKEGKIEFDSVTFHYNQGKKVFENKSVIIKPKQRVGLVGFSGSGKTT
                     FIHLILRLFNVESGKILIDGQDISQVSQDSLRSAISLIPQDTTLFHRTLMENIRYGNP
                     DASDDEVVMASKQACCDDFISLLPDGYNTLVGERGIKLSGGQRQRIAIARAILKNTKI
                     LILDEATSQLDSLTEEAIQNSLWQLMESKTTIVIAHRLSTLLHMDRILVFEAGKIIED
                     GSHEELLAKDGLYKSMWDAQVGGFLPESDRDRDNWDE"
     gene            14385..16016
                     /locus_tag="U6309_001285"
     CDS             14385..16016
                     /locus_tag="U6309_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEN5504734.1"
                     /translation="MEHETMEFDVIIVGAGPSGLSAAIKLKQLALASQKEITICILEK
                     GAQVGAHILSGAVLEPRSLKELLPENWQQAPLDTPVNKDLFYFLTQNKSFKLPTPKPM
                     HNEGNFIISLGELCKFLAEQAEALGCEIYPGFAAAEILYNDKNQVIGVATGDVGIDRQ
                     GKKSDNYQPGMHLLAKQTLFAEGCRGQLSQTLMSRYHLRDHSQPQTYGIGIKEIWQVD
                     ASRHEPGKVIHTVGWPLDHATYGGSFLYHLSEQRVAVGFVVGLDYKNPWLNPFGEFQR
                     FKTHPLIKSVLTGGERIGYGARALNEGGWQSLPKLTFPGGALIGDAAGFLNVPKIKGI
                     HTAMQSGMLAAQACFDALSEEKSGQFELSAYPEKINQSWLKQELYAVRNIRPGFKYGL
                     FPGLINAALETYVTRGYSPWTLKNHADHKSLIPAEKAKKINYPKPDGVLTFDKLSSVF
                     LSNTFHEENQPCHLKLKQPKLAIEVNLNQYASPESRYCPAAVYEIIEGVEKGPRLQIN
                     AQNCIHCKTCDIKDPRQNIVWQAPEGGGGPNYSGM"
     gene            16105..17877
                     /locus_tag="U6309_001286"
     CDS             16105..17877
                     /locus_tag="U6309_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEN5504735.1"
                     /translation="MYQPYLLFAILILSLILFVWNYWRYDFVALFALAISVLTGLVPF
                     NKAFSGFSNPAVITVGCVMILTYAITQSGVLNNSFIKLKKFSKKTSFQVGLYSGISAF
                     LSAFMNNVGALGIMMPIAIHAFTEIKKSPSIILMPIAFCSVLGGVITAIGTPPNLLIS
                     NFRNQVLGTPYNMFDFTPVGLPVALMGVLFVSLIGWKLVPVRAKYSKSGDSFQISDYM
                     TEVKVPETSSMCEKTVKEFLEMTKANFELIAVAHEGKKKFAYTDDTIIHCNDILIIEA
                     SPEDLKNILNSNGLILAGNKPLTSKELYDSDIITMEAVVLPDSSIEGQSASMMRFRSR
                     HKINLLAISREELSFRKKLIDIRFAAGDVVLLQGNAATLRETIVDLGFLPLAERDINV
                     HLTQKKFLPIICFTLSIILASLQILPLDFSFMIAVLALILLKAIPTHSLYRTIDWSVL
                     LLLAALIPVGEALQTTGAADLIANGFMKIAGQYSPIFALVAILVITMTLSDLLNNAAT
                     ALIMAPIAIKIAQSAHVNVDSFLMAVAIGASCSFLTPIAHQNNTMVMGPGRYRFSDYF
                     RLGLPLELLVVVVTIPTILWVWPL"
BASE COUNT         5537 a         3638 c         3212 g         5581 t
ORIGIN      
        1 cagaaacgtg cttttgaggg aataccaaaa ctactggcat gccgctaaaa acttaaagat
       61 tattaaataa aaaatctgtg ataagtatgt aaattgtgca aattattttg ttaatacggt
      121 atgaagcttg caggtgagtt ctaaaacttg tgttataatg atattaatta attgataaat
      181 taaacaatat atgaaattac tattcgattt ttttcctatc gttttatttt ttatagtcta
      241 taaatttttt ggcatttata ccgctactgc tgttgctatg gtagcctcat taactcaagt
      301 tgcgttttac agattaaagt ttcagcatta tgaaaaaatg catttgttta gtctggctat
      361 cattatggtc ttgggaggag cgacgttatt ctttcaaaac ccttggttta tcaaatggaa
      421 gcctacagga atctactggt tatctgcact tgtcttttat ggttcaggtt atatagggtc
      481 taagccacta atacaaaaaa tgatggaagc aaacatcaat ttaacaacaa aaatttggta
      541 tcgacttaat ttggcatgga cattattttt tatagtaatg ggggcattaa atttatatgt
      601 tgcctatcat tatgacactg atgtatgggt taattttaaa ttatttggtg gtgttgggtt
      661 tactttactt tttgtgttga ttcaagcttt ttatttaaca aaacacacag atgaaaaaag
      721 ctttgaaaaa caatagtaac atctctgtat ctgcagaaaa atatagcatg taataagaga
      781 aaaagtagga gcaccgatga gattattact tgttgaagac gatgaactgc ttggcgatgc
      841 agtaaaagcc ggactgacac aatttggtta cgttgttgac tggctaaaag atggcgaagc
      901 tgctcgggcg gctctcaaat cagagtcttt tgaattaatt attcttgact taggattacc
      961 taaattatcc ggtttatcat tattgcaaaa catcagacat gatggaaata gcacacctgt
     1021 gattattctt acagctcggg aatcaataga agatagagta aaaggcttag atagcggtgc
     1081 tgacgattat cttattaaac cttttgattt aaacgaatta agtgcaagga ttagagcatt
     1141 ggtaagacgc tctcagggca gagctgattc cgtgttgcaa tataaaaata ttactctcga
     1201 tcctgctgct cattctgtat tcgttgatga tgtactcgtc aatgttccaa gaagagaatt
     1261 tgctctattg cagaaactac ttgaaaacag tggtcaggtc ttatctcgag aacaattaat
     1321 gcagagtatt tatggttggg aagaagacgt ggacagtaat gctctcgaag ttcatatcca
     1381 taatttacgt aaaaaactta acgcaaactt tatccgcact atacgtggtg taggatatat
     1441 ggctgaaaaa aatgacagtg tacttctatc gtgaaatcgt ccattcgaaa atttttatta
     1501 attaatctgc ttcttgcaat tactattact acaacattga ctgccattgg taactattat
     1561 cttgatcaaa aagacattca agaccacctt gatacattga tggctatttc tgcgctatct
     1621 tatcaagcct tattagggga tgatcttcat caaagacctc ttgcaaaaat tcaaaatgcc
     1681 ctggaatcta ttccccaaaa aattaatact tattatcaaa aaagatattt aaatgatgag
     1741 cctcaagaaa attatttgga taaatttaat tttcaggtat ggaccaatgg aggtaaatta
     1801 ttattacatt cacctactgc ccctaaaata ccattaacag cagagacaga cggcttcagc
     1861 gataaaagaa tttctaacca ggattggcga gtattcacaa cttataatga caaagcagga
     1921 attcgtactg tactggcaga gcgttatgat accagaaatg agctgggtca cagaattgcc
     1981 caagatgacc tctatattat gttgcttact tttccattat caggactatt gatatggatc
     2041 attattggaa gaggactaga cagtctggat agagtggcag aagaggttgc taaccgtgca
     2101 ccaagtcacc tggaacctgt tgatttacaa gaagttcccg aagaaattaa acctgtaatt
     2161 gatgaattaa ataaattatt ttttcgactc aaagaagggt ttgagcgtga aaaacgattt
     2221 gctgccgatg cagcacatga gttgcgaaca cctcttgcag cacttaaggc ccaagctcaa
     2281 gtggctttga actccaatga tatagaagaa aaaaatcagg ctttacaaaa attaattgcc
     2341 agcgttaatc gaagcacaca tatagtgcag cagttgctaa ccatgagtaa acttgtacca
     2401 gaagcaacag aactacatga gcaagatgaa gtcaatctaa gtaaattaac tcgtgaaata
     2461 ttagccatgt tggctccgtc tgcggtcgaa aaacaaattg agctggaatt tgaaaccgat
     2521 gaaaaaatgc ctaaaattta tggaaatcca acggctttgg gaatattaat acgcaatctg
     2581 gttgacaatt ccattcgata ttccatggaa aacgggagag ttattgttcg attattaaaa
     2641 cataataacg aaatcatact cgaagtatgt gataatggcc cagggatccc acctgaatta
     2701 caaaaacgtg tttttgaaag attttttaga gtattaggga acaaaagccc gggtagcggt
     2761 ctgggtttgg ctatcgtgca acaaatatgc gaacttcatg gtggaagggt cgtgcttgat
     2821 tcgcctaaaa ttggcacagg cctaattgtg agagtttatt tgcccatcaa aacaaaacca
     2881 aaataaactc aataatcaaa tcataacctt gctgtaatta atctttttag cttggagaat
     2941 ctgcttcatg tttctgtgtg tcggtcgcag tcttcgcttc ttcttccttc ttttgagcat
     3001 tatagttcgc ttcctttaca ctgtttttat gctctttcat ctgttgggaa gtaacctttt
     3061 ctgtggtatt tgaagaggct gcatcggctg taatagccgt tgattgctct gtgtttctct
     3121 gaattgacat acctggggtc actttaccct tatcagtttg gtgtttatga gtattgactt
     3181 gagaatcagc agcattgaca gcaaaggtta gcaccacact caccataaaa ccgatcattc
     3241 ttttcataaa tgctcctgtt tattatcgag aattttatca gatcgcgcag ttacaatctc
     3301 atcgcgaaga aagaatttgc aaagcctcac gaatcaattg ctcacaactc ttattgccat
     3361 tatcaatttt attcacaact ttccacgcct cttgtggctt atagcctaat gcctccaacg
     3421 cgctgattgc ctcttgttga ctatgagtac tacctgcctg cttttggaaa gtatcggaaa
     3481 ctgaaccatc aaattgcttt atactgtctc tcatttcaac tactaagcgc tctgcagttt
     3541 tcttgcctat gccaggtaat ttggttaata aagcagcatt ctcctggtga atacactgaa
     3601 taaattcctt aggagaaata ctggataaaa cagtcatagc cagtttgggg cctaccccat
     3661 tcactttaat taatgatctg aataaagaac gctcctcttt atccaggaag ccatacaata
     3721 acaaagcatc ttctcgaact atggtgtgaa tatgtaatcc gataggttgg ttaccatttt
     3781 ctatttgaaa aaaagtattc aaagatgttt ctacatcata tcctactcca ttgacatcaa
     3841 gaaccaattt tcctggctga tgtttatcta taatttgacc atggagccag cctatcatag
     3901 tctcatcctc ctacgtttcg ttcctcttga accgattaat tttgacaaac catttctcat
     3961 atggctatga catattgcta ttgccaaagc atcggcagca tctttttgag gagctcgatt
     4021 gagctgaaga agctctacaa ccatatgact cacttgatct ttttcagcgg ccccataacc
     4081 aactatactt tgctttattt ctcttgctga atactcacag attttcgcac gatgtgaggc
     4141 tgctgctacc atggcaactc cccgcgcatg tcctaatttc aaagctgaat taggattgtt
     4201 atgcataaat atctgctcaa tagcaacctc agttggggaa tagtgatcca ttagctcaca
     4261 tattccatca tatatttgca ataatttatg acttaattcc gcatcgtttg atgtgcggat
     4321 acagccgcta tcgatgtacg tgattttacg gtcagattct ttaatcaatc catatcctgt
     4381 aacccgtgag cctggatcta tccctaatat aatcgtcatt aattcattga ttctaatatt
     4441 ttttcagaaa attcagcatt gctgtaaact tcttggacat catccaaatc ttccagcata
     4501 tcaatcagtt taatcaaact ttcagccgtt tcatcactaa ttggaatcaa ggtttgtgca
     4561 cgcatagtta aatgagactg ctcaacctcc aaaccagcat cctgtaaagc atttaagacg
     4621 gtgtgataag cttctacagg ggtaatgatc tctatctgac tgtcttctac agcaacatca
     4681 gaagcgccag catcgattgc aatctccatc accttatctt ccggttgatt agaagccatg
     4741 agaatttcac cttgattagt gaaaagatag gatactgaac catcagtgcc taaattaccc
     4801 ccatgttttg aaaaagcatg tcgaacttct gataccgttc gatttttatt gtcagacaaa
     4861 caatctacca atatagcaac gccacctggc ccgtaaccct cataacgcat agctatcatt
     4921 gcttcgccat caactccccc tacccctcgc ttgacagcat tatcgatggt atcgcgcttc
     4981 atattggcat tcagcgcttt tttaacagca tctctcaaac gtggattact tgactcatct
     5041 cctcctccca tacgggcagc tacagtaatt tcacgaatta atttagtaaa tatttttcct
     5101 cttttggcat cctgtacgcc tttgcggaat ttgatattag cccatttact atgaccagcc
     5161 attacatcac ctctaaatga atttttattt ggagattata caatgctcta acaccaaaaa
     5221 gaatgactaa agccatcgat gctctcgaca atttgaactt ttttggacta tgctagcacc
     5281 ataaaaaaat cttgaaggag attgaactgt gtatttgcaa ggatttggta attatcacca
     5341 cagcgaggct gttaaaggag cattaccccc aaatcaaaac tcaccgcaac actgtagctt
     5401 aggactttac gcagagcaat tgagtggaac ctcgttcacc cgtccccgac ataataatct
     5461 tcgaagttgg ctatatagaa tacttcctac tgttacacag ggcacgtatt acccctatga
     5521 gtttaatatt atgcaacctt ttgttgatga gttgtcaccc aatgccatgc gttggtcacc
     5581 tctttataac agctctcaaa ttaaatgtga ttttgttgaa ggactatttc atattgccgg
     5641 tagcccgtta gttaatacct atacttatta ttgcaaccgc tccatgagcg ataaatattt
     5701 cgccaataat gatggtgagt tattatttgt tccctatgca ggcgagattc atctgcatac
     5761 tgaatttggc aaattaatgc tctcttcagg atcgatcgca gtgatacctc gtggcgttaa
     5821 atttaaagta gaagtaatca gcaaggaggc aaaaggttat ctttgtgaaa atagcggaaa
     5881 tcccttaacc ttacctcaat taggccccat tggagccaat ggtttggcaa acccacgaca
     5941 ttttcaatac ccagtagccg catttgaaaa ttctggcggc gagcatacta taatctgtaa
     6001 aaaccagaaa aaattatggt ttactgtatg caaccactct cctttaaatg tcgtggcctg
     6061 gcatggcaat tatgcaccat attgttatga tctcagtttg ttcaatacaa ttaacacagt
     6121 cagttttgat caccctgatc cttccatatt cactgtatta acttcagaaa gcgaaatacc
     6181 cggtgtttct aacttagact ttgttatttt cccacctcgc tggatggttg ccgaacatac
     6241 ttttagaccc ccttattttc atagaaacta catgaatgaa ctgatgggac ttgtctatgg
     6301 tgaatatgac gccaagaagg aaggattcat accgggcggt atcagcatcc ataattgcat
     6361 gactccacac ggacctgatt atgaatctta cgaaattgca gcgtcgcagg atctaaaacc
     6421 aaattatatc aactccctgg cctttatgtt tgaaaccaaa gactactggc aagtaactga
     6481 gcaagcttat cggcatccca gcaggcaaat ggattatctt aattgttggc aaggctttaa
     6541 aatagagttt agtcaataaa ttgactgctt tttaagatgt aaaattgacg gcaattaatt
     6601 aatttggttt cagttgggtc aaatttgacc caacttgtgt gtaattaatt aactagttaa
     6661 atcaaaacaa atcctcttgt gttagtccaa cccgctctaa caaagttcta agtgtcttca
     6721 aagcttcaac ttgaatttgg cgaacgcgtt cacgggtcaa atcaatttcc ttaccaacat
     6781 cttccagtgt cgctttttca aaacctctta acccaaaacg tcttgcgata acctgctgtt
     6841 gattctcagt taatttatcc agaagagatt ctatatgctg ccttaaattc tcattggtta
     6901 gcagctctgc aggatttaaa ctgttttcat cagcaattgt atctaataaa gatttggttt
     6961 catcaaatcc aattggagta tcaactgaag cgactttatc attcaatccc aataattttt
     7021 ggacatcctc caaaggctta tccaccattt ctgctatttc ttcaggtgat ggttcatgat
     7081 ccagtttttg cgtcaattgt cttgctgctc tgagataaac attaagttct ttcacgacat
     7141 gaataggcaa gcggatagtt ctcgtttgat tcataattgc gcgctcaata gtttgcctaa
     7201 tccaccaggt cgcataagtt gaaaaacgga atcctcgctt ggggtcaaat ttctcaacag
     7261 acttcattaa acccaagtta ccctcttcga ttagatccag aagaggtaat cctctattaa
     7321 gatagcgtct ggctatttta accaccaggc gcaaattcga ctcaatcatt ttcttgcgtg
     7381 cttccatatc accttttaaa gcaagagtcg cataatggat ttcttcttca gccgttagca
     7441 aaggagaaaa accaatttca cccaagtaaa gttgagtcgc atctaaagcc ttggcaatat
     7501 ttttcccgcg ctggaatgaa ggaaaagctt catcatcagt aaactctgga agttcatcaa
     7561 gctcttcttc cattttttcg atatcaggtt caatatcctc ttctgacagc agagagtcat
     7621 catctggctc agaccattcc tcttctttaa attccttgtc cttaattggc tcttcttcat
     7681 cttgcattgt atcacctata tgtttagcta tgccttgaaa tagtttttac ttcttaacaa
     7741 gcctattgtt gctataacta tgcgttatat gtcaaccttt ttgtagataa ttcaatggat
     7801 taactggcac ccctgctctt ctaatttcaa aatgaacacc ccagtatgat ctatctatta
     7861 aaccagcctc tgctattacc tgccctgcac taacacgctg tccctctgtc accaaatttc
     7921 tggcattgtt accgtatgca gttaaatatt cattactgtg tttaataata attaaattgc
     7981 catacccaga caaaccgctg ccagcataag ccacaactcc actcgccgaa gctctaactt
     8041 tatctcctct attacatgca atatttatgc cttttttacc ctgagaaggg ataaatgttg
     8101 taacaacacg accagttact ggccacaacc aaccagagga agaacgtaca ttataagctg
     8161 gggaataaat aattttttgt tttggcttat gggtaatatg taagttagcc ctgggagctg
     8221 aatgaaggta atttgatttt ggcacgatgc cttttaaact taaaacttgg cctacacgca
     8281 aggaataagg tggcctcaga tgattgataa tcgcaagccg tctataatcc gtatcataac
     8341 gaaatgctat agcataaagg gtctcacctg atcgcaccgt atggaatttc tgatattttg
     8401 aataaggctg ccattttagc tcggtaacag gagccaaatc acttcttgtc ccacaaccag
     8461 caacacttaa gataaaaaaa atacaaatta attgtttgcg aaataggcgc atcattatct
     8521 catttattat ttattaattt tctatgcaaa tttcgttaag taagttagat aaatgatcaa
     8581 atagcttata gtgagtcatg tctaaatgaa gcggtgttat agaaacatgg cctgttttaa
     8641 ttgcaaaaaa atcagtaccg gcaccagcat ctgcttctat gcccggtgga ccaacccaat
     8701 aaatgggcct tcctctggga tcatattctt taataatagg ctcagcgcta tggcgagttc
     8761 ccaatcgcgt aacctgcaat cctcttattt gattcaaagg taaatcagga acattaacat
     8821 ttaatatagt ttgagaaggt aatttgttag cacttaattt tataaccaat tgtttggcaa
     8881 taattgcagc tgtttcataa tgttgtatgt tatctcctac cattgagatt gcaatagcag
     8941 gcaaccctaa gtaacgacct tccatagccg ctgccactgt acctgagtaa agtacatcat
     9001 cccctaaatt ggcaccctca ttaatccctg aaacgactat gtctgctata ggctctaaaa
     9061 atccggttaa cgccagatgg acgcagtcag taggtgttcc atccacacta taataaccat
     9121 tgtccaattg tttaactctc aatggttgtg ttaaagtcaa agaattactg gcgccacttc
     9181 tatttctatc aggcgcaacc actttaactt ctcctaaagt ggacagctca ttagctaaaa
     9241 ttttgatccc gggcgctaaa accccgtcat cattgcttac taatattttc atgtttaact
     9301 ctcaggggat tctaaccatt ttacaactgc taaatagtaa gcaagatctt cttgatttct
     9361 tttcaatgaa tcttgtaatt cagcgttatc ctttttactc tctatttccc tttgctgatt
     9421 ggcaataact tgctgcaaag ccaaaatttt gttaccaaac tcttgtggat tgtattgtag
     9481 ttgataagca agaccattca tcctcctgcc aattgcctca agttgctgac aacttaatcc
     9541 atcaggttgc gtgccagggc acaattttaa agcattttgc agttcagatg ggtgggaacg
     9601 ataataggat tcgtcttttt taaactgaca tgaaaccaat cccaaagtta ttaatattat
     9661 cactaagtac ttattcatac ccgccctctt gatgtaaact acaaaaagcc gatattatca
     9721 ccagtcagca agtaacgcta tcttatgaga ataccatatg cttgatttta gtttgaatcg
     9781 tgatccccaa acgggcgagt tatacataga aacctcatta tgtggaaagc ctttactgac
     9841 tacgccgcaa ttaaacaaag gaacagcatt tactcaggaa gaaagaaaag atttcggtct
     9901 tttaggcaag ttaccacacc gcgtagaaac tcttgatgaa caagtaaagc gtgcctatct
     9961 tcaatattca agttacacaa cacgtttgca gcaacatatt tatttaaata atttacatga
    10021 taaaaaccaa atagtatttt acaaactgct aagtcgtcat ttgggggaaa tgcttcccat
    10081 catctataca ccaatcgttg gtgctgcggc caagaggttt agtcatgaat acagacagcc
    10141 acgaggattg tatattgctc attcagataa aaaccagctt gaggaaataa ttaataaccg
    10201 ttcgaatcca gacatagacc ttgttgtagt gaccgatggt gagggagtcc taggcattgg
    10261 cgatcaaggg ataggaggaa tggatatacc tgttgcaaaa ctaatggttt acagcttatg
    10321 tggtgggatt gatccaactc gcacattacc catatttctt gatgtaggaa ctaacaacca
    10381 agaattatta aatgacccta tgtatctggg ttgccgtcac cccagaataa aaagctcaga
    10441 atacgacgat tttatcaaaa cttttgtcaa tgaaattcat aagcaatttc caaatgcttt
    10501 tttacattgg gaagactttg gtcgaggcaa tgctcgacga atcctggatc aatttcaaga
    10561 tgaattgtgt acttttaatg atgacattca gggaactggt gcagtaactc tggcagcaat
    10621 tttggcagca tgtgatgtaa ctggactacc cttacatgaa cacagaatcg ttgtgtttgg
    10681 agctggctca gcaggtactg gtattagcga ccaaattgtt gatgcgatgg tgaaaagcgg
    10741 attgagccta actgatgctt atgacagatt ctggttaata gacaaacagg gtttattact
    10801 tgcgactgac caagagctta ctgatgctca aaaaccctat gcacggaatc ctatagatat
    10861 acaatcctgg gaaattaata ataaacaaca tccttcgttt actgacacta tcaggcatgt
    10921 caaacctacc attttaattg gctgttcagc gcaaacgggt gctttctcac aagatattgt
    10981 ggaaactatg tcgacagcct gcgagcgacc aatcattttc cctttatcaa atcctgatga
    11041 aaaatgtgaa gcacagccct cagatatatt ggcatggagt aaaggacggg cattaattgc
    11101 tactggaact gcttttgccc ctattgaata tcagaaccga atggtgcaaa tagctcaatg
    11161 caacaatgcc ttggtattcc ctggaattgg attgggcgtt ttggcggtaa gcgcctctcg
    11221 tttaaccaaa gatatgatat tggctgcctc ccagacttta agtaaatttg cgccaagtaa
    11281 aaaggacagc tttttgcccc ttttaccctc attagacaat gcacaaattg tagccaaaga
    11341 aatagctatt gcagtagctc agtgtgcaat agactcaggt tatgctcaaa agaatcaaga
    11401 caaggatcta cctcgattaa ttgatgaact cttttgggag cctagatatc tgccttttag
    11461 aaaaagggga tagagcgaat aaaataaagc tctatgatta tattattgaa aaataaattt
    11521 tcttaattct tagtctgagg ggttgacaaa attaatcgtt acatttttta tagtcccgct
    11581 ctctggctcc caggaacgag cgtatttaaa gaccattttg gtttgagtgg gataggtttt
    11641 acctttattc aaattaaaag taaatagcat tttaccgcct gcacctatca aattagtctt
    11701 gggcttttca taaatgctat tacttaaggt aagcaaattt ttgtcaaatt ctactacaga
    11761 ccattgataa cctgtagttg gattagctgc aagactgaca acgaaactgg gattttttga
    11821 gctgacattg atgacaacct cgttatttgc ataggccaga gcagagaagc ccataagtaa
    11881 tccgctgaat aattttttca tatttttttc ctcacaaagc ttcttacacg ctcaccatta
    11941 tgggttacga tttacgttaa tctgttttaa tttcccataa tcaaaaatta attcgtattg
    12001 aaaatcaaca ggagattttt gataaatcca ggtttcgact gtattagtac cataacctat
    12061 ctgataacca gccgcgttgt ataatggcac attctcctca tagactaatt tatcaagtgg
    12121 ctcaccacat ttagacaaaa cagtgtattt actatcccct tcatagacta atttatctcc
    12181 acaccgcatt gcatacgcat taggatatac aatcattaaa aaggcaatag caaataaata
    12241 attccggatg atcattcccc accccttgag aactcaatgc tccttgcaac aaaattttca
    12301 aaaagtctat ttttatagca tagcacttgg tatctgatta ttcatcccaa ttgtctctgt
    12361 ctcgatcgga ttcaggcaag aatccaccta cttgagcatc ccacatggat ttatataaac
    12421 catccttcgc taataactct tcatggcttc catcttcaat tatttttcct gcttcaaaaa
    12481 ctaaaattct atccatatgc aatagtgttg ataatcggtg ggcaataaca attgtagtct
    12541 ttgattccat gagttgccac aatgaatttt gtatggcttc ttcggttagg gaatccagct
    12601 gtgaagtagc ctcatctaat atcaaaatct tcgtattttt taaaatagcg cgagcaattg
    12661 caatgcgttg tctttgcccc cctgatagtt taattcccct ttcacccacc aaagtattat
    12721 agccatcggg taaaagtgaa ataaaatcgt cacagcatgc ctgttttgat gccatcacaa
    12781 cctcatcatc actcgcatca gggttaccat aacggatgtt ttccataaga gtcctatgaa
    12841 acaatgtagt atcttgaggg ataaggctga tagctgaacg taaggaatcc tgagacactt
    12901 ggctgatgtc ctgaccatca atcaaaattt ttccagattc cacattgaat aggcgtaaaa
    12961 ttaaatgaat aaaggtcgtt ttaccgcttc ccgaaaaccc caccaaacca acacgttgtt
    13021 taggcttaat gatcactgat ttattttcaa atactttctt accctggtta taatgaaaag
    13081 ttacgctatc aaactctatt tttccttctt taacaatcaa tggcttggca tcaggagcat
    13141 cttctacctc tatcggtgca ctaagcaaag caatagcttg tcttgctatg cctatctctc
    13201 gaaagaactc ggcaatagca tggcacaaat accaaatttg agtcatgatt gaaaacgata
    13261 cattgaatat aaagaccaaa tctcctgttg taatgagctt tttattccaa aaagtcaaca
    13321 agaaatacac catagccact aacatcactg tgactggaat atcgaggtac aaacgaaata
    13381 tgttcatata aatcgacagc tttttattac tttgtacctc catattctga cttgctgaaa
    13441 tataagccag ctcatcgcga gaacgagcga aaagcttaac cacactggaa ttactcaaac
    13501 tgtcaactat tttaccgctt aactgacttt tatcctctgc gttttctttt gcatattgat
    13561 caactttttt agacaatcta tagctgatat aaagctgaaa aataatccaa cttaacaaaa
    13621 taactgcaaa ccagtagtta atggtacaca tcaaaatcaa tgccacaatg atagaagcga
    13681 atgctgcgat cgcataccag gttatgatat tgaatatgga atcaagagca cggggtaagt
    13741 cgttgacttt gttagctaaa ccccccgcca tttgattgga aaaataatga taagactggc
    13801 gacttaaata ctccatgaca gacattctga catcagcctg aaatcttggt atcacataac
    13861 cttgccacca attttgcaga cgtaaaatca caattaaggc gaaccaggca cctccaccta
    13921 accataaagc cggagcaact gctgaaaaaa tattttcctc cccggagtgc cccccaagag
    13981 catcaatgat cattttcaaa gaatagggaa tagcattatt ttcaagcacc atagcaatcg
    14041 gtgctacaaa aaacacagta aaagccaaag gctgtttctt tataaaatgc cagaaaaacc
    14101 gacccagtgt ttttggcaac tcttcagatt ttttgttata gctattcata aaccatacct
    14161 tcaatgaatt ttgacaaggt tcgattattc taacctaata tttgctaatt ggagaattta
    14221 tggttaaaaa acaattatat gccctatcaa ttcagctatt ctctcagatc tacattgcca
    14281 taaaataaaa ttcgcccttc aaaaattgaa aatataaaat ttaatcttga tcttaaacct
    14341 tataaaggcg atcatttttt catttgtaag ttagaggagt acgtgtggaa cacgaaacga
    14401 tggaatttga tgttatcata gtcggtgcag ggccttctgg attatccgct gcaatcaaat
    14461 taaagcaact ggctttagct tcacaaaaag aaattactat ttgtatactg gaaaaaggag
    14521 ctcaggtcgg cgctcatatt ctttctggtg cagttttaga acccagaagc ttaaaagaat
    14581 tactgccaga gaattggcaa caggccccgc tagatactcc tgtcaacaaa gatctgtttt
    14641 attttttgac tcaaaataaa tcatttaagc tccctactcc aaaacctatg cataatgagg
    14701 gaaattttat tatcagtttg ggagaactat gtaagtttct tgcagaacaa gcagaagctc
    14761 taggctgtga aatataccct ggatttgcgg cagcagaaat tctctataat gataaaaatc
    14821 aagtcatagg tgtagctact ggcgacgtag gaattgatcg acaagggaaa aaaagtgata
    14881 attatcaacc tggcatgcat ttattagcca aacaaacttt atttgctgag ggatgtcgcg
    14941 ggcaattaag tcaaacatta atgagtcgct atcatttaag agatcattca caaccacaaa
    15001 cttacggtat agggattaaa gaaatctggc aagttgatgc atcccggcat gaacctggaa
    15061 aagtcatcca tactgttggt tggcctcttg atcatgcgac ttatggaggc tcctttttat
    15121 atcatctttc agagcagaga gttgctgtag gttttgttgt aggacttgac tacaagaacc
    15181 catggttaaa tccttttgga gagtttcaga gatttaaaac tcaccccctt attaaatctg
    15241 ttctaacagg cggtgagcga attggttatg gagccagagc acttaatgag ggaggatggc
    15301 aatctctccc taaacttacc tttcctggtg gcgccttgat tggagatgct gccggtttcc
    15361 taaatgttcc taaaattaaa ggaattcaca ccgcaatgca atctggaatg cttgctgcac
    15421 aagcttgctt tgatgcacta agtgaggaaa agagtggtca atttgagcta agcgcttacc
    15481 ctgaaaaaat caatcaatcc tggctaaagc aagagctata tgcagtgcga aatattcgac
    15541 cgggattcaa atacggttta ttccccggtc taattaatgc tgccttggaa acttatgtaa
    15601 ctcgagggta ttctccatgg actttaaaaa atcatgcaga ccataaatcg ttaattccag
    15661 cagaaaaagc taaaaaaatt aactatccca agcccgatgg cgtgctcaca tttgataaac
    15721 tctcatctgt atttttatca aatacatttc atgaagaaaa tcaaccctgc catctcaagt
    15781 taaaacaacc taagctggcc attgaagtga acttaaacca atacgcttcc ccagaaagcc
    15841 gctattgccc agcagctgtt tatgaaataa tcgagggtgt tgaaaaaggg cctcgattac
    15901 aaattaatgc ccagaactgt attcattgta aaacgtgcga tattaaagat ccgcgtcaaa
    15961 atatagtttg gcaagcaccc gaaggtgggg ggggaccgaa ttactcagga atgtaatcaa
    16021 tcaaacttaa ataatgtttt tgagtagagt cctgtaatta ttagcaagga tgttgatata
    16081 aaacatgaaa ataaggatta ttgcatgtac cagccttatc ttttatttgc cattttaatt
    16141 ctttctctca ttttattcgt ttggaattat tggagatacg attttgttgc tctgtttgca
    16201 ctggccatat cagtattgac cggccttgtt ccctttaaca aggcattttc aggattcagt
    16261 aacccggcag taataacagt cggctgtgtc atgatcttaa cttatgccat cacccaatca
    16321 ggcgtattaa ataattcctt tataaaatta aaaaaatttt caaaaaaaac atcatttcaa
    16381 gtcggccttt atagtggtat cagtgcattt ctgtctgctt ttatgaataa tgtgggcgca
    16441 ttagggataa tgatgcctat cgccattcat gcttttacag aaattaaaaa atcgccatct
    16501 atcatcttaa tgcctattgc cttttgctca gttttgggag gagtaatcac tgccataggt
    16561 acccctccca acctattaat ttccaacttc cgcaatcaag tcctgggtac cccatacaat
    16621 atgttcgatt ttactcccgt agggcttccg gtagcattga tgggtgttct ctttgtttca
    16681 ttgattggat ggaaattagt tccagtgcgg gccaaatata gtaaatcggg agattcattc
    16741 cagatttctg attacatgac tgaagtaaaa gttcctgaaa cctcttctat gtgcgaaaag
    16801 actgttaaag agtttctcga gatgactaaa gcgaactttg aactgatagc agttgcgcat
    16861 gaaggtaaaa aaaaatttgc atacactgat gatacgatca ttcattgcaa cgatatttta
    16921 attattgaag cgtctcctga agatttgaaa aacattttga attcaaatgg tctaatcctc
    16981 gctggcaaca aaccattaac atctaaagag ctctacgaca gtgatatcat cactatggaa
    17041 gctgttgtat taccggattc cagtattgaa ggacaatccg cttccatgat gagatttcgt
    17101 tcacgtcata agattaattt gttagctatt tcaagagaag aattatcgtt tagaaaaaaa
    17161 ttaattgata ttcgtttcgc agcaggagat gtggtactac ttcagggaaa tgccgcaaca
    17221 cttcgcgaaa caattgtcga cctggggttc ttgccccttg cagaaagaga tattaatgtc
    17281 catttgacac aaaaaaaatt tctaccaatc atttgtttta cactatccat cattttggca
    17341 tctctgcaaa tactaccact cgacttttct tttatgatag cagtgcttgc gctcattcta
    17401 ttaaaagcaa taccaacaca cagtttgtat cgtactattg attggtcggt actattacta
    17461 ttagctgctc ttatccctgt tggagaagca ttgcaaacga ctggtgctgc agatttgatt
    17521 gcaaatggat ttatgaaaat tgctgggcaa tattccccta tctttgcctt ggttgcaatt
    17581 ctggttatca ctatgacttt atctgattta ttaaacaatg cagcaacagc attaattatg
    17641 gctcccattg caatcaagat tgctcagtca gcgcatgtta atgtggattc tttcttaatg
    17701 gcagtggcta ttggtgcttc gtgttccttc ctgactccaa ttgcccatca aaacaacacc
    17761 atggttatgg gtccaggaag ataccgattt tctgattatt tccgtttggg attaccatta
    17821 gaactccttg ttgtagtcgt tactattcca actattttat gggtatggcc attataaaaa
    17881 tagaaattaa tatgcattaa acctgtacca taatcgaaaa aacactatgg tacaggttag
    17941 agatatttga atagacgcaa atggtata
//
LOCUS       DASLOE010000046        17955 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.046, whole genome shotgun sequence.
ACCESSION   DASLOE010000046 DASLOE010000000
VERSION     DASLOE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17955
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.046"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1344)
                     /locus_tag="U6309_001287"
     CDS             complement(<1..1344)
                     /locus_tag="U6309_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN5504736.1"
                     /translation="MKKNRFTYGLALGALGVVFGDIGTSPLYALKVTLSGIPINQFNI
                     LGVLSLIFWSLIIVVSFKYLMIIFRADNDGEGGILALLALMKHKSTKYQPLFYIVAIF
                     GAGLLLGDGMLTPAISVVSAVEGLGTLSDKLYPYVLPIASVILVLLFSLQAKGTARIG
                     YLFGPLILIWFITIAILGILQIVEHPVVLQAINPYHAIAFLVDEGLRGYFLLGGIFLV
                     VTGGEALFADIGHFGKNPIRFSWFFIALPCLLLNYFGQGANLIVRPEEISNPFFMIAP
                     PWFYLPLIIIATVATVIASQAVISATFSLTKQAVLLGLCPKIPIVQTSMLHSGQIYVP
                     QINFILFIGTMAFCLAFKTSDNLAHAYGIAVNLEMLLVDAMVAYAAVSIWRWSTFNVM
                     FLFGLFLLIDLAFLGANTHKFITGGWVPIVLAFFIAFIMYSWRYGLEYLRDNFYMN"
     gene            complement(1372..2355)
                     /locus_tag="U6309_001288"
     CDS             complement(1372..2355)
                     /locus_tag="U6309_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504737.1"
                     /translation="MRQFTKYLAIFLFSSSVSVAYAGPWFTGPLLAPAGHTVAKGHTN
                     LEVYGIDVFTDGQYNASGQRISTPKFKTFAANPILTHGFTDWLDVQLTVPYLFNSTRG
                     VHENHLADVSTALGIQILEQKGSPKRIDFRVLLQETIPTGRYDQLNPALLGTDATGLG
                     SYQTLVGLDFQYLLQVFKTHYLRTRFIASRLFSSNVHVDGLSSYGGSINTHGTVKPGI
                     ENNLDLAFEFTLTQHWVAVMEGYIATGKATRFNGILNFNSIGSPPVNVSDLDAAPVVI
                     GSGSFREEALAPAIEYNFNENIGVIGGVWFPVSGVNTSHYMTYVLAINAFW"
     gene            2433..3605
                     /locus_tag="U6309_001289"
     CDS             2433..3605
                     /locus_tag="U6309_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEN5504738.1"
                     /translation="MKAKVILVNGKQNTVLRGHPWIFPKAIAQYSGKLITGELVEIIG
                     SEGESLGFGAYNEHSLYRVRVLAHSNEQINSFDYKSLITHRIKQASLVRRCLDLPNEQ
                     TNAYRLFNSEADGLSGLTIDCFNDYCVVASSAYWVELNKSIIVQVLQELLPHNHIIWL
                     PQNKPLSQDGWKEIHTEKNQGSTQVSEEGVLYHVEFAQTQKTGLFLDQRENHKRIAKL
                     SRGKRVLDLYTFTGGFALHAAKAGALHVTAVDSSAQAIEQATNNAVLNHLNTIEFIKA
                     DAREYLKMAGEYDVLILDPPKLVPSQKHLQKAKNYYRFLHREAFKYMKPGSLLMTCNC
                     SSALSSQDFCSLVSVQAVAMGKQARILGVYGPASCHPTLAAFPEGNYLTAVLLALV"
     gene            3727..4875
                     /locus_tag="U6309_001290"
     CDS             3727..4875
                     /locus_tag="U6309_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEN5504739.1"
                     /translation="MSLALKIKSIILSFFLFLMATIVCASEVSLRDKIGQMLILGFQG
                     KVVDEKSPIAQAIEKNNIGGVILFDFNQQSQTFDKNIESPEQVNRLNSQLQILTQKAN
                     RKYHRDNLPLLISVDYEGGNVNRLHPRYGFPEMPSAKDVGSMSLERADASAELMANTL
                     KSTGFNLNFFPELDVNINPDNPIIGKKDRSFSSIPEVVTQYAQLYTEQFIKHQIHCSY
                     KHFPGHGSSLSDSHLGFVDISDTWSEQELIPFLQLLSQPNHCDMVMIAHIVNRKLDMT
                     GLPASLSYQIINGLLRRDLKFDGVVITDDMQMKAITNYYGLETAVTLSINAGADMLIF
                     GNQLVEKFQDSTEIIDMIEQKVRSGEISEQRINEAYQRIVKMKRSFNY"
     gene            5016..5741
                     /locus_tag="U6309_001291"
     CDS             5016..5741
                     /locus_tag="U6309_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEN5504740.1"
                     /translation="MKNVLKREDAANKKLGVLIYENVQPMDVIGPWEVFATWKTILDA
                     PIDMFLVAETSNEVCCASQISLKPHCEFNNSPQFDYLLVPGGVGRREQVNNTRLISFL
                     KKQAQNTDYLLSVCTGAFLLHAAGLLTHHEATTYWRAMPELKSFEDVQLVEKRIVKSG
                     KVWTTGGISSGIDLAFEFISTIAGNDVAGKVQLLFEYFPECKLYSQPAMVSELPSYYA
                     TKKDEPVLEMNLPDYIKEYFKKS"
     gene            complement(5830..6453)
                     /locus_tag="U6309_001292"
     CDS             complement(5830..6453)
                     /locus_tag="U6309_001292"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEN5504741.1"
                     /translation="MSDLDINSLDWQKMNGLLPAIIQNAENGKVLMLGYMNQEALIAT
                     LTTGQLTLYSRSRKRLWRKGESSGNSMALQHISADCDNDSLLIQVIPKGPACHLGYST
                     CYQPHNNYTLGFLDGLIELINERAEEKNPNSYTAQLLDSGVNRCAQKLGEEAVETVIA
                     AVSNNKEELINEASDLIYHLLVLLKACELSFYDVLLCLQARDRSVHT"
     gene            complement(6450..7217)
                     /gene="hisF"
                     /locus_tag="U6309_001293"
     CDS             complement(6450..7217)
                     /gene="hisF"
                     /locus_tag="U6309_001293"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEN5504742.1"
                     /translation="MLTKRIIPCLDVLDNQVVKGVKFRNHRIIGDILPLAAHYSTSGA
                     DELVFYDITASSDDRSVCPVWVNKVAAIINIPFTVAGGIRTLHQARSILNAGADKISI
                     NSPALENPSLINELSKEFGKQCVVIGVDSQWIIDDYYVYQYTGNENKSTNSKRKTREW
                     IREIQDRGAGEIVLNCMQSDGVRMGYDIAQLSEMINLCQVPLIASGGAGKINDFVEVF
                     KKSHVSGALAASVFHDKILTINDIKLALQTENIEVRL"
     gene            complement(7211..7930)
                     /locus_tag="U6309_001294"
     CDS             complement(7211..7930)
                     /locus_tag="U6309_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HEN5504743.1"
                     /translation="MLVIPAIDLQSGRCVRLKQGRFDQVTQFSVFPLERALYFAKLGA
                     KRLHVVDLDGARRGKMQQLELICSMQKTGIAIQAGGGIRSIEQALECSNAGISKLVIG
                     SLAITNPDLTIQIIEKIKPENIVLALDVRVDTKVPLLAINGWQNNSTSSLWEVVSYYK
                     NYGIKHILCTDIACDGMMNGPNFDLYQQAVEYFPQIAWQASGGIRHMQDITTLDSLGI
                     SAVILGLMLYQDNVNFEELLC"
     gene            complement(7924..8523)
                     /gene="hisH"
                     /locus_tag="U6309_001295"
     CDS             complement(7924..8523)
                     /gene="hisH"
                     /locus_tag="U6309_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEN5504744.1"
                     /translation="MIAVIDVSGNNLTSLTNALIRLGGHFALTHDAEEIQKASHVILP
                     GVGTARSGMTALQQNGLIDVLRTLTQPLLGICLGMQLLLEYSEEDDIPCLGLIPGVAE
                     LLKAERNHPVPHMGWNNLHWQKTSSLQQGLNNSDYVYFVHSYALKADDYALARCQYHE
                     EFTAVVKKGNFYGMQFHPEKSADVGMALLNNFLSLESTC"
     gene            complement(8520..9578)
                     /gene="hisB"
                     /locus_tag="U6309_001296"
     CDS             complement(8520..9578)
                     /gene="hisB"
                     /locus_tag="U6309_001296"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HEN5504745.1"
                     /translation="MKKILFIDRDGTLVEEPFDFQVDSLDKIKLTSGVIPALLQLQKA
                     GFTFIMVSNQNGIGTVAFPEEDFAVCHEFILDLFSSQGILFDEIFICPHTPEDNCICR
                     KPKTGLLEPYLKETAFAKHYSWVIGDRDTDKEFADNLGVNFLPISKTHTWEMVVSAII
                     NDARKASVQRKTKETAIDLSVQLDSDQTSVIDTPIPFFTHMLEQVAKHGGFDLRLQAS
                     GDLEVDEHHLVEDTAIALGEAIRTALGDKWGINRYGYTLPMDESLATIAIDISGRSFC
                     DFKGQFSREFVGGMATEMVPHFFQSLSSALGATIHIQVTGANHHHMIEACFKVLGRAL
                     RQACSRTNNSLPSTKGVL"
     gene            complement(9556..10650)
                     /gene="hisC"
                     /locus_tag="U6309_001297"
     CDS             complement(9556..10650)
                     /gene="hisC"
                     /locus_tag="U6309_001297"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEN5504746.1"
                     /translation="MSILNLVRTDLLNSQNYVPGGESARYRSHANELPWSPVTMGEHN
                     LNYYPNIGLQIELQNQLAKRYQIYSDQIILTRGSDDGIDLTTRFFLTAGKDAFMQFPP
                     TFPMYAFYVRLQQAELIECPLDRRTNFRLTLDQIENSWKPNCKVIMFCSPNNPTGNLV
                     DLNLIAKTCELYANQSIIVVDEAYIEFANAPSATSLIGEFENLIVLRTLSKAFGLAGL
                     RLGCIIAQSPIIQAFNKIIAPYSIATPSMELAKRALNNSDWFTKTIEQIKSSRAWVIK
                     KFADNPIIEKIYPTETNFILIQTRFSKQLTTWLARYGIAVRDFPSSSLLHDHLRITVG
                     NDEQNQLLINALSSFNADVAGLNYEKDFIY"
     gene            complement(10651..11946)
                     /gene="hisD"
                     /locus_tag="U6309_001298"
     CDS             complement(10651..11946)
                     /gene="hisD"
                     /locus_tag="U6309_001298"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEN5504747.1"
                     /translation="MLTIKNWQLLSDNDKRRCLSRPRDNSAIKENVLEIINQVQLSGD
                     KALYDLTKRFDRVNLQYLQISQEKIRQASIPQNALIAIKQAIETISSYHQFLLPENTE
                     ISTASGITIRNVYRPIQKVGLYVPGGNKTPLVSSLLMQAIPAKVAGCPIKVLCTPPDA
                     EGEINEHILVAARLCGIDTIYAIGGAQAIAAMAYGTESVIKVDKIFGPGNSYVTQAKT
                     LVAIDADGAAIDMPAGPSEVMILADTEANPEFIAADLLAQAEHGPDSQVILICDECEL
                     ANQVNQQLEIQMSYLSRIEFIKQSLTNSRIIICSNQSEQLDIINSYAPEHLIINRKNP
                     EPWVEKIVAAGTVFLGSWAAETMGDYVTGSNHVLPTSGFARNHSGLSTLDFMTRFTVQ
                     AINQEAIRNLGPAAMTLAELEGLDAHANAVQIRLNTLGD"
     gene            complement(11952..12833)
                     /locus_tag="U6309_001299"
     CDS             complement(11952..12833)
                     /locus_tag="U6309_001299"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEN5504748.1"
                     /translation="MKNRLRLALQKKGRLADESLNLLQRCGLKFRIKPNALLTHVDNF
                     PIDLLFVRDDDIPTLVFDSLCDGGIVGENVLLEAFLNRPDKSFRKILTLGSCTCRLSI
                     AVPEAFDYQGSGSLDGKRIATSYPNLLNNYLAERKISAEILALSGSVEVAPRMGMADV
                     ICDLVSTGQTLEDNKLKEVDTVLNSQAVFIQTQRQLDSDMQELFAMLARRIQAVQQAQ
                     ERKYIVFHAPKAALERIAQKLPGAESPTILPLPGNNEKVAVHVVSSEGVFWNTLETIQ
                     YLGASSILVLPIEKMLE"
     gene            complement(12830..13126)
                     /locus_tag="U6309_001300"
     CDS             complement(12830..13126)
                     /locus_tag="U6309_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN5504749.1"
                     /translation="MKIHGSFKKSALPNLMQAISLLKNEDEAMQFFTDLCTPAELESM
                     ADRWQVVPLLRQGIPYRTIHEETGVSVTTITRVARCLSFGTGGYRLIAERLEKL"
     gene            13328..13498
                     /locus_tag="U6309_001301"
     CDS             13328..13498
                     /locus_tag="U6309_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504750.1"
                     /translation="MKPLTRDIVFTLSFKMILLVLLWYFCVRGMHPDLSSNKEWLLGK
                     EEQTVLTPNNKR"
     gene            13503..15035
                     /locus_tag="U6309_001302"
     CDS             13503..15035
                     /locus_tag="U6309_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEN5504751.1"
                     /translation="MIPAAEVVDLSRLQFALTALYHFLFVPLTLGLSVMLAIMETVYV
                     MTGRELWKQMVKYWGILFGINFVLGVATGLTMEFQFGTNWAYYSHYVGDVFGAPLAIE
                     GLMAFFLEATFVGLFFFGWNRLSKLQHLTSTWLLALGTNLSALWILIANGWMQHPVGA
                     YFDYQTMRMEVSSFYEMMFNPVAQAKFVHTISAGYVTGAMFVLSISAYFLLRGRNKAF
                     AKRSMTVAVSFGLASALSVVVLGDESGYVANENQKMKLAAMEAMWHTEKAPANLTIFG
                     IPDEKSFTTKYAIDIPYALGIIATRSFNTPLQGIIELIEEGKLKIKDGMIAYDALKVL
                     QKDPKNEQAKAVFTKHSENLGYALLLKKYTENVTDATEEQINQAAHDLKPKVLPMFFS
                     FRIMVACGIYFILLFATGFYLSVRHRLEKTSWYHRVAFYSLPLPWIAAELGWIVAEYG
                     RQPWVVEGILPTFMGASSLTSGQVMTSIAGFVLFYTVLAIVEIYLMVKYIRLGPDGIN
                     HH"
     gene            15051..16187
                     /gene="cydB"
                     /locus_tag="U6309_001303"
     CDS             15051..16187
                     /gene="cydB"
                     /locus_tag="U6309_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEN5504752.1"
                     /translation="MPLDYETLRIIWWLLIGILLIGFAIMDGFDLGVAMWLPWLGRTD
                     IERRILINSIGPTWEGNQVWFILGGGAIFAAWPTLYALSFSGFYLAMLVVLLALILRP
                     VGFKYRSKLDNPIWRAFWDWALFIGGFVPALIFGVAVGNVIQGVPFYFDESLRVFYTG
                     SFFELLNPFALGCGLLSVLMLAMHGAFFIQVKTVGTLQNKAKDRARWSALLVILLFIG
                     LGAWVYYGIDGYVLKGTVPHDGPSNPSYKDVIIQAGAWFNNYKEYPVSLLVPVLCVIS
                     AFLAIIFANKSVLAFVLSGLSVGTLIATVGVSMFPFILPSSSNPKYSLIVWDSSSSQV
                     TLFIMLVATVLLLPIVLAYTAWVYRVLRGKVTESSIKAEQDSAY"
     gene            16199..16300
                     /gene="cydX"
                     /locus_tag="U6309_001304"
     CDS             16199..16300
                     /gene="cydX"
                     /locus_tag="U6309_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HEN5504753.1"
                     /translation="MWYFSWILGTGLACVFAVLNAVWLELREEDVKQ"
     gene            complement(16413..17045)
                     /gene="pyrE"
                     /locus_tag="U6309_001305"
     CDS             complement(16413..17045)
                     /gene="pyrE"
                     /locus_tag="U6309_001305"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEN5504754.1"
                     /translation="MNHTKSSFIKLALECQVLKFGEFTLKSGRISPYFFNAGLFYHGD
                     SLRKLGQFYAKTLLEQEVSFEHLFGPAYKGIPLATATAVALAELGRDITVTFNRKEVK
                     THGEGGQLIGSPLTGRTVIIDDVITAGTAFRESQTLIKENGGILRGVIIALDRCERGL
                     TEKSTLSEIREQGIEVYSIINLFDLIEFLKNDNQYEQVQKLESYQERYGA"
     gene            17294..17527
                     /locus_tag="U6309_001306"
     CDS             17294..17527
                     /locus_tag="U6309_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEN5504755.1"
                     /translation="MSQRETGHVKWFNEKKGFGFIVNQKGEDIFVHYKDIQGVGFKTL
                     HENDPVTFELDRGPKGLKAQNVVVASESVHTEE"
BASE COUNT         5356 a         3348 c         3649 g         5602 t
ORIGIN      
        1 gttcatataa aaattatctc ttaaatactc taacccgtag cgccaactgt acataatgaa
       61 ggctataaag aaagctaaaa caattggaac ccagccacct gtaatgaatt tatgagtatt
      121 agctcctaaa aaggctaaat caatcaaaag gaataggcca aagagaaaca tcacattgaa
      181 tgttgaccat ctccatattg aaacagcagc ataagcaacc atggcatcga ctaaaagcat
      241 ttcaaggttt acagcaattc cataagcatg agccaggtta tccgaggttt tgaacgccag
      301 gcaaaatgcc atggtaccga taaatagaat aaaattgatt tgcggaacat agatttgtcc
      361 tgaatgcagc atggaggtct ggacaattgg aattttgggg cataaaccta acaacacggc
      421 ttgtttggtt agtgaaaaag ttgctgaaat tactgcttga gatgcgatca ctgtagcaac
      481 agttgcaata ataattaaag gcagataaaa ccatggtggc gcaatcatga aaaatgggtt
      541 gcttatttct tcggggcgta ctatcaggtt agcgccttgg ccaaaataat ttaatagcag
      601 gcaaggaagc gctataaaaa accagctgaa tcggattgga tttttaccaa aatgacctat
      661 gtctgcaaat aaggcttcac ctcctgttac tacaaggaaa atacccccca gcaaaaagta
      721 tcctctcagg ccttcgtcaa ccaaaaacgc aatggcatgg taggggttga tcgcttgcaa
      781 aactactgga tgctctacaa tttgcaatat tcctaatatg gctatggtta taaaccaaat
      841 aagtataagt ggcccaaaaa gatagcctat ccttgctgtg cctttggctt gcaaagagaa
      901 aagcaaaacg aggatcacag aagcaatggg gagaacgtaa gggtagagct tatcagaaag
      961 agttccaaga ccttctaccg cactgacaac ggaaatggcc ggggtaagca taccatcacc
     1021 taataacaaa ccggcgccaa atatggcgac tatgtaaaat agaggttgat atttggtact
     1081 cttatgcttc attaaagcaa gaagtgccaa aattcctcct tccccatcat tatctgctcg
     1141 aaaaataatc attagatact taaaagaaac aacgatgatt aatgaccaaa aaataagcga
     1201 taatacgcct aatatattga attgattgat aggaatgcct gataaggtaa cttttaaagc
     1261 atacagtggg ctagtaccta tatcaccaaa tacaacccca agagctccga gggcaaggcc
     1321 ataagtgaac ctgttttttt tcatttcata ggaggaataa cgcatgtgct atcaccaaaa
     1381 agcattaatt gccagtacat aggtcatata atgggaagta tttactccac tcacgggaaa
     1441 ccaaactcca ccaattactc caatgttttc attgaagttg tattctattg ccggggctag
     1501 tgcttcttct ctaaatgagc cgctgccaat aactacgggg gcagcatcca aatcactgac
     1561 attgaccggc gggcttccaa tgctgttaaa gtttaaaata ccgttaaatc gagtagcttt
     1621 accagttgca atataacctt ccattacggc aacccagtgt tgagtaagag taaattcgaa
     1681 agccaagtct aagttatttt caatgccggg tttaaccgtc ccatgagtat ttattgaacc
     1741 accataactg cttagtccat caacatgaac attactggaa aaaagacgtg atgcaataaa
     1801 tcgtgttctg agataatggg ttttaaaaac ttgtaataaa tattgaaagt ctaagcccac
     1861 taacgtttgg tagcttccaa gaccagtggc atcagtacca agtaaagccg gattcaattg
     1921 gtcataacgt ccagtgggta ttgtttcctg taaaaggaca cgaaaatcga ttcttttagg
     1981 ggaacctttt tgctccagga tttgtattcc caatgcagtt gaaacatcag ccaaatggtt
     2041 ctcatggact ccgcgtgtcg agttgaataa ataaggcacg gtcaactgca catccagcca
     2101 gtcagtaaag ccatgagtga gtattgggtt ggcggcaaag gttttaaact tgggggtgga
     2161 tattctttgt ccagatgcat tgtattgccc atcagtaaag acatcaatgc cataaacttc
     2221 gaggttggta tgacccttgg cgacagtatg gcctgcgggt gccaataaag gtccggtgaa
     2281 ccagggaccg gcataagcaa cgctcactga agatgaaaat agaaaaatgg ctaggtattt
     2341 tgtaaattgc cgcatatttg tccttctttc cattgcaagt ataattcttg atagtgtaga
     2401 ttaactgact tttaggtttt gggaaataga gaatgaaagc gaaagttatt ttagtgaatg
     2461 gaaagcaaaa cactgtatta agaggtcatc cctggatttt ccccaaagcc atagctcaat
     2521 attctggcaa gctaattaca ggtgaattgg ttgagataat tggctctgaa ggtgagagtt
     2581 taggattcgg agcctataat gagcactctt tatatagagt aagggttctt gctcactcca
     2641 atgaacagat aaatagtttc gattacaagt ctttaattac tcatagaatc aagcaagcca
     2701 gtctggtgag acgatgtctt gatttgccaa atgaacagac taatgcttat cggctgttca
     2761 acagtgaagc ggatggactc tctggtttaa cgattgattg ctttaatgac tattgcgttg
     2821 tagcaagttc tgcttattgg gtagaattga ataaatcgat tattgttcag gtattgcaag
     2881 agttactccc gcataatcat atcatttggc ttcctcaaaa caagccatta tctcaagacg
     2941 gctggaaaga aattcataca gagaaaaatc aaggtagcac tcaggtttcg gaagaaggtg
     3001 ttctgtatca tgttgaattc gcgcaaaccc aaaaaactgg attgtttctt gaccaaagag
     3061 aaaatcacaa aaggatagca aaactatcca ggggaaaaag agtcctggat ctttatactt
     3121 ttacgggagg atttgctttg cacgcagcga aagcaggcgc tcttcatgtt acggctgtag
     3181 atagctcggc acaggcaata gagcaagcta caaataatgc tgttttaaat cacttaaata
     3241 ccattgaatt tattaaggca gatgccagag aatatctgaa aatggctggc gaatatgatg
     3301 tgctcattct tgacccgcca aaactggttc cttcacaaaa acatttacag aaagcaaaaa
     3361 attattaccg atttttacat agagaagcat ttaaatatat gaagccagga tctttattga
     3421 tgacttgcaa ttgttcttca gcgctttcat cccaggattt ttgttccctg gtaagcgttc
     3481 aggctgttgc gatgggaaag caggcgcgta ttttaggcgt atacggacca gcaagctgcc
     3541 atcctacctt ggcagccttt ccagagggaa attatttaac agctgtttta ttggcattgg
     3601 tgtaaaaaaa cttgattgtt aaatcatgga tgtcacagac cgattttcct gtgtaacatt
     3661 tgattagttt ggtcttaata tgtataattg gcgcatgttg caatagagag aattaatcat
     3721 tttctaatga gtttagcatt gaaaatcaag tcaattattt tatctttttt tctttttttg
     3781 atggctacaa tagtttgcgc ctcggaagtg agtttgagag ataaaattgg gcagatgtta
     3841 atattaggat tccagggcaa agtggttgat gagaaatcac ctattgccca ggcaattgag
     3901 aaaaacaaca ttggtggtgt tattcttttc gattttaatc aacagtcaca aacttttgat
     3961 aaaaatatag aaagtccaga acaagtcaac cgattaaata gtcagttaca aatactaacg
     4021 caaaaagcca accgtaaata tcatagagat aatttaccat tacttatttc agttgattac
     4081 gaggggggaa acgttaatcg attgcatccc cgttatggat tccctgaaat gccttcagca
     4141 aaagacgttg gttccatgtc tctggaaaga gctgatgcat cagcagaatt gatggcaaat
     4201 actttaaaat caacaggatt taatttgaat tttttccctg aattagatgt gaatataaat
     4261 cctgataatc ctatcatagg taaaaaagac aggagtttct cttcgatacc ggaagttgtc
     4321 actcaatacg cgcaacttta tacagagcaa tttataaaac atcaaataca ttgttcttat
     4381 aaacattttc ctggacatgg cagttcatta tctgattcac atttgggttt tgtagatata
     4441 agtgacactt ggagcgagca agaattaatc ccttttttac aattattatc tcaacccaat
     4501 cattgcgata tggtgatgat agctcatatt gtgaatagaa aactggacat gacagggcta
     4561 cctgccagcc tatcttacca gatcattaac ggattattgc gccgtgattt aaaatttgac
     4621 ggggttgtta tcaccgatga tatgcaaatg aaagcaataa cgaattatta tggattagaa
     4681 actgctgtaa ctctttcaat taatgcagga gcagatatgc taatatttgg taatcaattg
     4741 gtagaaaaat ttcaagactc gacagaaatc attgacatga tcgagcaaaa ggttcgatcg
     4801 ggagagattt ctgaacaaag gattaatgaa gcttatcaac gaattgtaaa aatgaagagg
     4861 tcttttaatt attagaaatt tttctcaatt tactctgaca aaagcacttt gataatctgc
     4921 gagctataga taagctgagg catatatgcg atgcaggtca attatcaaaa aaactaatat
     4981 aaaaaatggg atttttcaga ttagggtggc attgcatgaa aaacgtgctg aaaagggaag
     5041 atgcggcgaa taaaaaattg ggagttttga tttatgaaaa cgtccagcct atggatgtta
     5101 ttgggccgtg ggaagtattt gcaacctgga aaactattct tgatgcccct attgatatgt
     5161 ttctggtagc agaaaccagc aatgaagttt gttgcgcaag tcaaattagc ctaaaacctc
     5221 attgtgaatt taacaattct ccacaatttg attatttact ggttccaggt ggggttggta
     5281 gaagagaaca agtaaataat acgcgtttga tttctttttt gaaaaagcag gctcaaaata
     5341 ctgactattt gctttctgtt tgtactggag catttttatt gcatgcggca gggctattga
     5401 ctcatcatga agcaacaact tattggcgcg ctatgcctga attaaagtct tttgaggatg
     5461 tccaattggt tgaaaagcgt atcgttaagt caggtaaagt ttggactact ggtggaattt
     5521 ccagtggcat tgatctggca tttgaattta tttcaaccat tgctggcaat gatgttgcgg
     5581 gaaaagtaca gttgcttttt gaatacttcc ctgagtgcaa gctgtattcc cagcctgcta
     5641 tggtgagtga gttgccttct tactatgcaa ctaaaaagga tgagccagtg ttggaaatga
     5701 atttgcctga ttacattaaa gagtatttca agaaaagtta atttcttaat agtagtttaa
     5761 aaaattaact agtcttgatt gataattact ggagattctg gttatagaga tcttctatgg
     5821 aataagcaat taagtatgta cacttctgtc tcttgcctgt aaacacaata aaacatcata
     5881 aaaacttaat tcacaagctt tgagaagcac taataaatga taaattaaat cagacgcttc
     5941 gttgataagt tcttctttat tgttactcac agcagcgatc actgtttcta ccgcctcttc
     6001 tcctaatttt tgagcacatc ggttcacacc agaatccaag agttgagcag tataactgtt
     6061 tggatttttt tcctcagcac gttcattaat taattcaatc aaaccatcaa gaaaaccaag
     6121 agtataatta ttatgtggct gatagcaggt tgaataacct aaatgacaag cgggtccttt
     6181 aggaataacc tggatcaaaa gactgtcatt gtcgcaatcg gcgctaatat gttgcagagc
     6241 catactattg ccggaagact ctcccttacg ccacagtctt ttccgacttc tgctgtataa
     6301 ggtcagttgt cctgtagtta atgtggcaat taatgcctcc tgattcatat aacctagcat
     6361 caatacttta ccattttctg cgttttggat tattgccggc aatagtccat tcattttttg
     6421 ccaatcaaga gagttaatgt ccaagtcact catagtctta cctcaatatt ttctgtttgt
     6481 aatgctagtt ttatgtcatt gattgtcagt attttgtcat gaaaaacgct tgctgccaaa
     6541 gccccgctga catgagactt tttaaatact tcaacaaaat cattaatttt tccagcaccg
     6601 cctgatgcga tgaggggaac ctggcagagg tttatcattt ctgatagctg tgcaatatca
     6661 tatcccattc gaacgccatc tgattgcatg caattaagaa ctatctcgcc agcaccgcgg
     6721 tcttgaattt cacgtatcca ttctcgagtt ttacgttttg aattggtact tttattttca
     6781 ttgcctgtat actgatagac ataataatca tcaattatcc actgactatc gacccctatt
     6841 accacacatt gttttccaaa ctctttgctc aattcgttaa tcaatgaagg gttttccaac
     6901 gccgggctgt tgattgagat tttatctgct ccggcattta atattgatct tgcttgatgc
     6961 aatgttcgga tgcctccagc cactgtaaat ggaatattaa taatagccgc cactttattg
     7021 acccaaacag gacaaacaga acggtcatcg ctacttgctg taatatcata aaataccaat
     7081 tcgtctgcgc ctgatgttga gtagtgagca gctaatggga gtatgtctcc aattattcta
     7141 tggttgcgaa attttactcc cttgacaacc tgattatcca gaacgtcaag acagggaatg
     7201 attcgcttgg ttaacataat aactcctcga aattcacatt atcttgataa agcattaacc
     7261 caaggattac ggcggatatt cctaatgaat ccagcgttgt aatatcctgc atatggcgaa
     7321 ttccccctga ggcttgccag gcaatctgag gaaaatattc aactgcttgt tgatacagat
     7381 cgaaattagg cccattcatc atgccatcgc atgcgatgtc agtacaaagt atgtgcttta
     7441 tcccatagtt tttgtaatag gacactactt cccaaaggct gcttgtacta ttattttgcc
     7501 agccattaat cgcaagaagg gggacttttg tatcaacacg aacgtccaga gcaagaacaa
     7561 tattttcggg tttaatcttt tcaatgattt gtattgtcaa atcaggattt gtaatagcca
     7621 ggctgccaat caccaatttt gaaattcctg cgttgctgca ttccaaagct tgttctatgc
     7681 tccttattcc acctccagct tgaattgcta tccctgtctt ttgcatggaa caaatgagct
     7741 ccaactgttg catttttcct ctacgagcac catccaaatc gactacgtga aggcgttttg
     7801 cgcctaattt ggcaaaatac aaagcccttt ctaggggaaa aacgctaaat tgcgttactt
     7861 gatcaaaccg tccttgtttt aagcgcacac atcgtccaga ttgcaaatca atagctggaa
     7921 taactaacat gtagactcca aagataaaaa gttatttagt aatgccatgc caacatcggc
     7981 tgatttttct ggatgaaatt gcatgccata aaaattgcct ttttttacaa ctgcagtgaa
     8041 ctcttcgtga tactgacatc gagccaatgc ataatcatct gcttttagag catagctatg
     8101 aacaaaataa acataatcac tattatttaa tccttgttgt aatgatgatg ttttttgcca
     8161 atgaagattg ttccatccca tatgaggaac aggatgattt ctttctgctt ttaataactc
     8221 tgcaactcca ggaattaaac ccaggcaggg tatgtcgtct tcttcgctgt attcaagtaa
     8281 taattgcatt ccaagacaga tgccaagaag tggttgggtt aaggttctta aaacgtctat
     8341 tagcccattt tgctgcaaag ctgtcatacc agaacgtgct gttccaaccc ctggcaaaat
     8401 gacatggctt gctttttgta tttcttcagc atcatgggtc agtgcaaaat gccctcccaa
     8461 tcgaatcaag gcatttgtca gagaagttaa attattccca ctgacatcaa tcacagcaat
     8521 cataatacac ccttggttga aggtagagaa ttattcgttc ttgagcatgc ctgtcgcaag
     8581 gctctgccta aaactttgaa gcatgcttcg atcatgtggt gatgattggc gcctgttacc
     8641 tggatgtgga tagttgcccc caacgcacta gatagcgatt ggaaaaagtg aggcaccatt
     8701 tctgttgcca taccgccaac aaattcacga gaaaactgac ctttgaaatc acaaaaactt
     8761 ctgccactga tatcgattgc aatggtagct aatgattcat ccataggaag ggtatagccg
     8821 taacgattaa tcccccattt gtcaccaaga gctgttctaa ttgcttcccc caacgcaatg
     8881 gccgtatcct caaccaagtg gtgctcatct acttccaaat ctccagaagc ttgtaatcgc
     8941 aaatcaaagc ctccgtgttt tgccacttgc tccaacatgt gggtaaagaa ggggataggg
     9001 gtgtctataa cactggtttg atcgctatcc agttgaactg acaaatctat tgccgtctct
     9061 tttgtttttc tttgcacaga cgctttgcga gcatcgttga taattgcaga aaccaccatt
     9121 tcccaagtat gtgttttcga aatgggcaag aaattaacgc ccagattatc agcaaactct
     9181 ttatcagtat cccggtcgcc aattacccat gaataatgtt tggcaaatgc tgtttctttt
     9241 aagtaaggtt cgagcaagcc agttttaggt tttctacaaa tacaattgtc ttcaggggtg
     9301 tgagggcata taaaaatctc atcaaaaaga atgccttgag aggaaaacag atccaggata
     9361 aattcatggc aaacagcaaa atcctcttct ggaaaagcta cggttcctat gccattttga
     9421 ttggatacca taatgaaggt aaagccagct ttttgtaatt gcaatagggc tggtattact
     9481 ccagaagtta atttaatttt atccagagaa tctacttgaa aatcaaaggg ttcttcaact
     9541 aaagtgccat cgcggtcaat aaataaaatc tttttcataa tttaatcctg ctacatcagc
     9601 gttaaatgag gataaggcat taattaataa ttgattttgc tcatcattac ccaccgtaat
     9661 acgaagatga tcatgcaata aagaggacga aggaaaatct cttactgcga tgccataacg
     9721 agcaagccaa gtggttaatt gttttgaaaa tcgtgtttga atgagaatga aattggtttc
     9781 cgtaggataa attttttcaa tgattggatt atcggcgaat ttttttataa cccaggcacg
     9841 agatgatttg atttgctcaa tagtttttgt gaaccaatcg ctattattca aagctctttt
     9901 agccagctcc atgctaggag tggcaatgga atagggggca atgattttat taaaagcttg
     9961 aataatagga ctctgggcta taatgcagcc taagcgtaat ccagccagcc caaaggcttt
    10021 cgacagggtt cttaagacaa tcagattttc aaactctcca attaaggacg tagcgcttgg
    10081 tgcatttgca aactctatat acgcttcgtc cacaactata atagattgat tcgcatatag
    10141 ttcacatgtc ttggcaatta aatttaaatc caccaaatta ccagtcgggt tattgggact
    10201 acaaaacatg ataactttgc agtttggttt ccagctattt tcaatttgat ccagggtgag
    10261 gcgaaaatta gtccttctat ccaaagggca ttcgatcaat tctgcttgtt gtagccgaac
    10321 ataaaaagcg tacataggaa aagtaggggg aaattgcatg aaagcgtctt ttccggcagt
    10381 tagaaaaaat ctggttgtca aatcaatgcc atcatcactt cctctggtca gtatgatttg
    10441 atcggaatag atttgataac gttttgccaa ttgattttgc agctctattt gtaagccaat
    10501 gtttgggtaa taatttaaat tatgctcccc catagtaact ggtgaccaag gcagttcatt
    10561 ggcatgtgat cgatagcgag cactctcacc accggggaca taattttgac tatttaatag
    10621 atctgtacga acaagattga gtatggacat ttaatccccc agagtattga gtcttatttg
    10681 aacggcattg gcatgagcat ccaaaccctc taattccgct aaagtcattg ccgctggacc
    10741 taaattacgg atagcctcct gatttatggc ttgcacggta aatcgtgtca taaaatcaag
    10801 agtagaaagc ccgctatggt ttctggcaaa accactggta ggtaagacgt gattgcttcc
    10861 tgttacatag tcacccattg tttctgcggc ccaagagcca aggaatactg ttccggcagc
    10921 cacgattttc tcaacccatg gctcggggtt ttttctgtta atgatcaggt gttccggagc
    10981 gtaggagttg ataatatcca gctgttccga ttgattagaa cagataatta tcctgctatt
    11041 cgtgagagat tgtttgataa attctattct tgataaataa ctcatctgaa tttctagctg
    11101 ttgattaacc tggtttgcca attcacattc atcacaaatt aaaataacct gtgaatccgg
    11161 gccatgttca gcctgggcta ataaatctgc agcaataaat tcagggtttg cctcagtatc
    11221 tgcaagaatc atcacttcag atggcccagc gggcatgtca atcgcagctc cgtcggcgtc
    11281 aattgcaact aaagtttttg cctgagtgac atagctgtta cccggtccaa atattttatc
    11341 cactttgatg acactttcag tgccataagc catagccgct atggcttgag ctccacctat
    11401 agcgtaaatt gtgtcaatac cacataatct tgcagctacc aggatatgct cgttaatttc
    11461 cccttctgca tcagggggag tacaaagcac ttttattggg cagcccgcta cctttgccgg
    11521 tattgcctgc atgagaagtg aagaaactaa aggtgtttta tttcctccag gcacatacaa
    11581 gccaactttt tggattggtc tgtagacatt gcggattgta attcctgatg cagtcgaaat
    11641 ctcggtattt tcaggcaaca aaaactggtg atatgacgag attgtttcaa tggcttgttt
    11701 tatggcgatt aaggcatttt gcggtatgct ggcttgtcta attttttcct gtgagatctg
    11761 taaatattgt aaattgactc tatcaaaccg tttggttaaa tcgtaaagtg ctttatctcc
    11821 tgataattgg acctgattta taatttccag cacattttcc tttattgcag aattgtctcg
    11881 tggccttgaa aggcatcgcc ttttatcgtt atcagacagt aattgccagt tttttatcgt
    11941 tagcatatga attactccag catcttttca ataggtaata ccaggatgga gctggcacct
    12001 aaatattgga tagtctctaa agtattccaa aacacacctt cactggagac tacgtgcacg
    12061 gcaacctttt cattatttcc gggtaatggt agaatggttg gtgattcagc tcctggtaat
    12121 ttttgagcaa ttcgctcaag ggcggccttg ggcgcgtgaa aaacaatgta ttttctttcc
    12181 tgagcttgct ggacagcctg tattcttctg gcaagcatgg caaacaactc ttgcatatca
    12241 gaatccaatt gtctctgagt ttgaataaag acggcctgac tgttgagaac agtatcaact
    12301 tctttgagtt tattatcttc gagtgtttgg cctgtagata ccaaatcgca tatgacgtca
    12361 gccattccca ttcgtggcgc tacctcaaca gagccggata aggccagaat ttctgcacta
    12421 atttttcgtt cagcaagata attgttgagt aaatttggat agctcgtggc aattcgcttc
    12481 ccatccagac tcccggagcc ttgatagtcg aatgcctctg gtactgctat ggataaacgg
    12541 caagtacaac tgcctaatgt taatattttt ctaaatgact tatccggcct gttaagaaag
    12601 gcctccaaca ggacattttc tcctacgatg ccaccatcac ataaactgtc aaatacaaga
    12661 gtagggatgt catcatcacg aacgaagagt aaatcgattg gaaaattatc aacatgggtt
    12721 aataatgcgt ttggcttaat gcgaaatttt aaaccacaac gttgtaggag atttaaagac
    12781 tcatctgcta atcgaccttt tttttgcaag gccagacgta aacgattttt cacaacttct
    12841 ccaatctttc agctattaaa cgataaccac ctgtaccaaa gcttaaacaa cgagcaactc
    12901 gcgtgatggt tgttacactg acccctgttt cttcatgtat cgttctgtaa gggattcctt
    12961 gacgtagcaa aggaactact tgccatcgat cagccataga ttctagttcg gcaggagtac
    13021 ataagtctgt aaaaaactgc atggcttcgt cttcattttt aagcaagctg atggcttgca
    13081 tcaaattagg cagcgcagat tttttaaaag atccatgtat tttcatgtta atgcattagc
    13141 atgttgtaac atgttaatga tgataatgat tctatttatc attgtcaata agttagtgaa
    13201 aaaaaaatct ggatagggta gagtatacag gtctctttcc cggaaactgg attggctgaa
    13261 aatcaaaagg cagacgcaaa tattttttaa cagagactgt cgatatgata aatcctgagg
    13321 aaatgtcatg aaaccactta ccagagatat tgtatttacc ctttcattta aaatgatatt
    13381 gcttgtttta ttatggtatt tctgtgtcag aggaatgcat cctgatctgt ctagtaataa
    13441 agaatggttg ttaggaaaag aagaacaaac agtacttacc ccaaataaca agaggtagtt
    13501 ttatgattcc agctgctgaa gttgttgatt tatcaagatt acaatttgca ttaactgcac
    13561 tatatcattt tctatttgtg ccccttacgt tggggttatc ggttatgtta gcgatcatgg
    13621 agacagttta tgtaatgact ggccgggaac tctggaagca aatggtcaaa tactggggta
    13681 ttttgtttgg gattaatttt gttttgggag ttgcaaccgg tctgactatg gaattccagt
    13741 ttggtactaa ctgggcttac tattcacatt atgttggaga tgtatttggt gctccattag
    13801 caattgaagg actaatggcc ttttttctgg aagctacttt tgtggggtta tttttctttg
    13861 gctggaacag attgtcaaaa ttgcagcatc tgacctctac atggctattg gctttaggta
    13921 ctaatctttc agcattatgg attttaatcg ctaatggttg gatgcaacat cctgtaggtg
    13981 cctattttga ttaccaaacg atgagaatgg aagtcagcag tttttacgaa atgatgttta
    14041 accctgtcgc tcaggctaag tttgtgcata ctatcagtgc gggatatgtg acaggagcca
    14101 tgtttgtctt gtccataagc gcctattttc ttttgcgggg acgaaataaa gcgtttgcca
    14161 aacgctcaat gacagtcgca gtttcttttg gtttggcatc agcattgtct gttgtggtat
    14221 tgggagatga gagcggttac gttgccaatg aaaaccaaaa aatgaaattg gctgcgatgg
    14281 aagcgatgtg gcataccgaa aaagctcccg ccaatttgac tattttcggg ataccagatg
    14341 agaaaagttt cacgacaaaa tatgcgatag atattcctta tgctcttggt attattgcta
    14401 ctcgttcgtt caatactcct ttacagggca ttattgaatt aattgaggaa ggaaaattaa
    14461 aaatcaaaga tgggatgatc gcatacgatg cattgaaagt tcttcagaaa gatccaaaga
    14521 acgaacaagc caaggctgtc tttacaaagc acagtgaaaa tctgggttat gctttgttgc
    14581 tcaagaagta tactgaaaat gtaacggatg ccactgaaga gcaaataaat caagcagcgc
    14641 acgatctcaa acctaaagta ctgccaatgt tcttctcatt tagaattatg gttgcttgcg
    14701 ggatatattt tatacttctt ttcgcaaccg gtttttattt gtctgtaaga catcggttgg
    14761 aaaaaacatc ttggtaccac agggttgcat tttactcttt gcctttacct tggatagcgg
    14821 ctgaattagg gtggatagta gcagaatatg gtcgccaacc ctgggttgtt gaaggtattc
    14881 tgcctacttt tatgggagcc tcctctttaa catcagggca agtaatgaca tcaattgcag
    14941 gctttgtatt gttttatact gttcttgcaa ttgttgagat ttatttaatg gtcaaataca
    15001 tacgtcttgg cccagatggg ataaatcatc attgatgaaa ggagaatcaa atgcctttgg
    15061 attatgaaac actcagaata atttggtggt tattaatagg tattttgctt attggttttg
    15121 ccattatgga tggatttgac ctcggtgtgg caatgtggct tccctggtta ggtagaacag
    15181 atattgagag acggatatta attaacagca ttggcccaac atgggagggt aaccaggttt
    15241 ggtttatact gggcggaggc gctatttttg cggcttggcc tactctctat gccttatctt
    15301 tctcaggttt ttacttggct atgctggttg ttcttttggc gttgatttta agacccgttg
    15361 gctttaaata cagatcaaag ctggataacc ccatatggag agcattttgg gattgggctt
    15421 tattcatcgg tggatttgtt ccggctttaa tctttggggt agcggttgga aatgtcatac
    15481 aaggtgttcc attttatttt gatgagagtt taagagtatt ctatacggga agtttttttg
    15541 aattactcaa tccgtttgct ttagggtgtg gattattatc tgtgcttatg cttgctatgc
    15601 atggtgcttt ttttattcaa gtaaaaactg tagggacttt gcaaaataaa gcaaaagaca
    15661 gggctcgctg gagtgctctt ttggttattc ttttatttat tggattaggg gcttgggttt
    15721 attatggtat tgatggttat gttttaaaag gaacggttcc ccatgatggg ccatcaaatc
    15781 cttcttacaa ggacgtcatt attcaagccg gagcatggtt taataattat aaagagtatc
    15841 cagtatcact tttagtgcct gttttatgcg taatatccgc ttttttagcg attatttttg
    15901 cgaataaaag tgtgttggct tttgtgctga gcggattgag tgttggtact ttgattgcaa
    15961 cggtaggggt tagtatgttt cctttcatat taccctcttc ttcaaatcct aaatacagtt
    16021 tgatcgtttg ggatagctct tccagccagg ttactttgtt tattatgtta gtggcaacgg
    16081 tattattatt gcctattgtg cttgcataca ctgcttgggt ttatcgagtg ttacgaggta
    16141 aggtaactga aagttctatt aaagcagagc aagattctgc atattaggag gtttttctat
    16201 gtggtatttc tcctggattt tgggcacagg actagcttgt gtgttcgcgg tgcttaacgc
    16261 agtatggttg gaacttagag aagaagatgt caagcaatag acagaggcaa tatcagtgta
    16321 agttgctgag tttcatggat agctcagcaa ctgaaaacac aatgagtgat gattgtctta
    16381 ctctttttta aggaaaatct gtaatcctaa tattatgcgc cataacgctc ttgataggac
    16441 tccagttttt gtacctgttc gtattgattg tcatttttca gaaattctat tagatcaaac
    16501 aaattgataa ttgaatagac ttcaattcct tgctccctga tttctgacag ggttgatttc
    16561 tcagttaatc ctcgttcgca tctgtccaga gcaataatga caccacgaag aatccctcca
    16621 ttctctttaa tcagggtttg tgattctctg aatgctgtgc ccgctgtaat cacatcgtca
    16681 atgatcactg tccttcctgt tagcggcgaa ccaattaatt gtccgccttc tccatgagtt
    16741 tttacttctt tacgattaaa agtcactgtg atgtccctgc ctaactcagc caaggccact
    16801 gcagttgcgg tagcgagtgg gatacctttg tacgctggtc caaacaagtg ctcaaaagaa
    16861 acttcttgtt ctaaaagggt ttttgcataa aattgaccta acttacgtaa ggagtcaccg
    16921 tgataaaata aaccagcgtt aaagaaatag ggacttattc gtcctgattt taaagtaaat
    16981 tcaccaaatt taagtacttg acactccaga gctaatttta taaaagatga tttggtatga
    17041 ttcattctgg tttcctaata caaaatttta ttgaaaacaa tcacatattt taaaaaaatc
    17101 cttaacaatc aacaaaaaac tgatatgcta atcatagtgc aggatttatc cttgcatgga
    17161 aaagggttta ttatagcgta aattgtttat tagggagtga tttatttttt cactttaagt
    17221 tgcgctaaag tgaactttta tttatttaaa taacaatttt gatgtatctg tgtaagtcaa
    17281 aggggaaatg cgaatgtcgc aaagagaaac aggccatgtt aaatggttta atgaaaaaaa
    17341 aggatttggt tttattgtta atcaaaaagg cgaagatatt ttcgtgcatt acaaagacat
    17401 tcagggagtt ggttttaaaa cgctgcatga aaatgatcca gttacctttg aacttgatcg
    17461 aggacctaag ggtttgaaag cacaaaatgt ggtagtggct agcgaatcag tacacacaga
    17521 agaataagcc agatgatttt atctggtgag gctacgcttt attttttaaa gcctcgttaa
    17581 agtacttaag agcatgttta tagaaacctg agagtttgac ataaaatccg gtaattttat
    17641 agtctactgc cgagtcaaaa agtacagctg ttccaaatct aaaatgaatg gagaaggtgg
    17701 ctgacttgag gacgagtgta agttaaagag aatggtctga ggcgatcttg cttcggagtg
    17761 cagcgtcgaa ctcttaggct tatccacgta tgcaatcccc ccaaaaaaag catcaacgtc
    17821 ctgttgttat atgctcatta agtttcagat atgaagtatt tgtatgtcaa ctcaattcat
    17881 tttttatacc atttttttag tctggtttta ataattttat ttttattgtt aattcaagtg
    17941 cttatcattt ttcta
//
LOCUS       DASLOE010000047        17380 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.047, whole genome shotgun sequence.
ACCESSION   DASLOE010000047 DASLOE010000000
VERSION     DASLOE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17380
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.047"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1396)
                     /gene="sidM"
                     /locus_tag="U6309_001307"
                     /gene_synonym="drrA"
     CDS             complement(<1..1396)
                     /gene="sidM"
                     /locus_tag="U6309_001307"
                     /gene_synonym="drrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector guanine
                     nucleotide-exchange factor DrrA/SidM"
                     /protein_id="HEN5504756.1"
                     /translation="MSIMGRIKMSVNEEQFGSLYSDERDKPLLSPTAQKKFEEYQNKL
                     ANLSKIIRENEGNEVSPWQEWENGLRQIYKEMIYDAFDALGVEMPKDMEVHFAGSLAK
                     AQATEYSDLDAFVIVKNDEDIKKVKPVFDALNNLCQRIFTASNQIYPDPIGINPSRLI
                     GTPDDLFGMLKDGMVADVEATAMSILTSKPVLPRYELGEELRDKIKQEPSFSNMVSAK
                     KFYNKAIKDFTAPKEGAEVVSVKTHIMRPIDFMLMGLREEFNLYSEDGAHLSAPGTIR
                     LLREKNLLPEEQIARIESVYNQAMSKRFELHAEHKKEHDEMPYSDAKEMLDEVAKIRE
                     LGVQRVTRIENLENAKKLWDNANSMLEKGNISGYLKAANELHKFMKEKNLKEDDLRPE
                     LSDKTISPKGYAILQSLWGAASDYSRAAATLTESTVEPGLVSAVNKMSAFFMDCKLSP
                     NERATPDPDFEVGKS"
     gene            1466..2989
                     /gene="sidD"
                     /locus_tag="U6309_001308"
     CDS             1466..2989
                     /gene="sidD"
                     /locus_tag="U6309_001308"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector deAMPylase SidD"
                     /protein_id="HEN5504757.1"
                     /translation="MVYYAIIKDIVFTYNLQFTHLIHNDRISEVNLGGVTMRSIITQI
                     CNGVLHGQTYQSGSNDLDKGNSEIFASSLFVHLNEQGKEIIKHKDSDDKIVIGYTKDG
                     MAFQIVVDGFYGCERQAVFSFIDNHVLPLIDNFSLDLARYPNSKNVTESFIHTIYSLR
                     SKHAPLAEFTMSLCVTYQKDDQLFCAGFGIGDTGIAIKRNEGTIEQLVCHTEVDGFKD
                     AFDNYSSTNIDSVIERNSVFNTKVMPGDELVGYTYVPPALEITEKEFEVETVDGKKIN
                     KRIVRHLNLDPGNFDDKDPLFSQLLQVVKSKQKQLVEQAKETGQIQRFGDDFTVGRLV
                     IPDQLLINQLRMHALSIRVSDGLLSYIKNENENKGFLGIYGFFTGADKNIEKATLYKN
                     LIAKYQNNPFTSLIILSALVSDSKTPVMTQYLVDYLDFPSKASLANKITALLLKELEN
                     PDMREILGSRLATDVIEELETKIIRYIHNPTGSDIHSTLNLWTADKIKAATNSSLTI"
     gene            complement(3018..3497)
                     /locus_tag="U6309_001309"
     CDS             complement(3018..3497)
                     /locus_tag="U6309_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEN5504758.1"
                     /translation="MSLIKILGIGSPFGDDQVGCLAADCLKAELSKEQYISKSVCIES
                     HDRPGLRLIELMNSAKVAFLIDAVKSGCAIGTIHRLKNKEIYAFKNMLSTHEMGVAQA
                     LQLGHSLNALPEHIILYGIEIDSIAFNAMLSKPVEKAMRAVVIKIKKELKDMLNHIV"
     gene            complement(3494..4786)
                     /locus_tag="U6309_001310"
     CDS             complement(3494..4786)
                     /locus_tag="U6309_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEN5504759.1"
                     /translation="MGKDISINVPILARVEGEGALELCIRDSKIDTLKLKIYEPPRLF
                     EKFLEGRSYNEVLDFVARICGICPVAYQMSATQAIEQCFNLSPTPWVRDMRRLFYCGE
                     WLESHSLHVYFLALPDFLGFQSAPEMARKYPEEVKCGLRIQAFGNDLIKLLGGRSVHP
                     VGACVGGFYKAPSHSQIKLILDKAKDRVSDCEALIAWLASLSLPDNSHDFTSVSLYHP
                     TEYPMNEGRIVSDRGLNISKEEFDSYFKEYHVDYSNALHCLLQNKPYLVGPLARINNC
                     FGHLPVPIHLLLQSLSIQFPSRNMCHSIIARAVEMYYCVLEAIRILEQYDLPEQSRPE
                     IKPQAGEGAGCTEAPRGMLWQHYVFDETGQVLQARIVPPTSQNQARIEEDLKHSLIQF
                     GLNQEEEAIRYFSEMIIRNYDPCISCSTHFLNLKIDRI"
     gene            complement(4779..5564)
                     /locus_tag="U6309_001311"
     CDS             complement(4779..5564)
                     /locus_tag="U6309_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfhydrogenase subunit delta"
                     /protein_id="HEN5504760.1"
                     /translation="MKPRIAVHKFTSCDGCQLAFLNAGESLLTLSDLVEIVHFAEAGM
                     VDVDMKVDIAFVEGSISTPDEEHRIRKIRENSQYLITIGACATAGGIQALRNFADSRE
                     WIASVYASPDYIKTLTTSTAIAHHVKVDWELWGCPVNSGQILEAIRFLLSNATPKIKR
                     DSECMECKRKGNVCVLVTKKQPCMGPVTQTGCGSLCPTLGRACYACYGPKENANPNSL
                     GEWFLSIGLTKEAIARQFLHINNQAPAFNQAGNYFKGIKISNG"
     gene            complement(5582..6427)
                     /locus_tag="U6309_001312"
     CDS             complement(5582..6427)
                     /locus_tag="U6309_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEN5504761.1"
                     /translation="MNKSDYDPYLPREVEIVQRTQESSSIFTLHLRFTDEEHHKQFQF
                     YPGQFNMLYLYGVGEVAISIVSDPRKKTFLSHTIRAVGRVTKAMQKLQVGDRLGIRGP
                     FGVGWPLQKTIGKDIIVLTGGLGCAPSVSIINYILGRRRHYGKLSILQGVKHSEDFIF
                     RKQYAKWQKSDHTEVYIAADQAGPKWPWGVGYVTDLIDHIIIQPDNSVVMMCGPEMMM
                     NTAVKVFTQKGILENDIYLSMERNMECGIGHCGHCQYGGLFVCKDGPVFAYSQIKELF
                     KEPGF"
     gene            complement(6420..7559)
                     /locus_tag="U6309_001313"
     CDS             complement(6420..7559)
                     /locus_tag="U6309_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEN5504762.1"
                     /translation="MNDKTASFLPHDKLQHLIDSLQNAGYSCVGPQVRDGAIVYDVLT
                     RAEQLPWGMRDHQEPGGYRLERIPERKAFAFANGPQGIKPLLFKPQETVWKVERDEQG
                     KLCFKPHQPDETPVAIIGSRACDLAAMSIQDKVFIESNKPDPRYKRRREQLFVVAVNC
                     TYSSANCFCVSAGTGPAVNNSFDILMTEIEDGFVVEAGSDKGKRIINQLDLHDAKSAQ
                     CTDAQNNVKQAAALQTKRIPLDNKRDLRDLLFSNLNHTRWDDVAERCLSCGNCTLVCP
                     TCFCHSEVEKPGLDGCNSEHHREWDSCFTTGHTYLNGKIIRDDTKKQYRQWLTHKVGS
                     WFDQFDTSGCVGCGRCVTWCPVGIDITEELAVISGESNVRKIESE"
     gene            complement(7596..8654)
                     /gene="hypE"
                     /locus_tag="U6309_001314"
     CDS             complement(7596..8654)
                     /gene="hypE"
                     /locus_tag="U6309_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEN5504763.1"
                     /translation="MSEILITKNLTGPKLNFKEGTINLTHGSGGRAMAQLIEQLFLTA
                     FDNEWLAQKNDQACFIVPSGRMVIATDAHVISPLFFPGGDIGSLSVHGTVNDVAMSGA
                     KPLYLTASFILEEGFPLNDLRKIVYSMADAARQAAVAIIAGDTKVVERGKGDGIFITT
                     TGVGIVPEGMHISGDAAKPGDKIIVSGFIGDHGVAIMAHRNNLEFQTRVQSDTASLHE
                     LVGEMVKAVPDLHCLRDPTRGGLATTLNELASQSKVGFVIDESKIPIRTEVASACELL
                     GLDPLYVANEGKLIAICPSEKAERLLIAMKAHPLGLHSKIIGEVIEDHRCFVQLKTKL
                     GGLRIVDWLAGEQLPRIC"
     gene            complement(8651..9757)
                     /gene="hypD"
                     /locus_tag="U6309_001315"
     CDS             complement(8651..9757)
                     /gene="hypD"
                     /locus_tag="U6309_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEN5504764.1"
                     /translation="MKYIEEFRNGAYAQAIVNEIIATAEPQRHYQFMEFCGGHTHAIH
                     RYGIPSLLPNNVEMIHGPGCPVCVLPVARIDKAIALASMPQVILCSYADMLRVPGTGQ
                     RSLLTAKAGGADVRMIYSANDALKIAQENPDKDVVFFAIGFETTTPPTAFVIKQAKKL
                     NITNFSVFCNHVLTPIAMDSLLQANEVALDGFVGPAHVSIVIGSYPYEHVCKKYQKPI
                     VISGFEPLDVLHSILMLIQQLNEGRCEVEIQYTRAVNRTGNLLSQHIMTEILEIRDRF
                     EWRGLGFIPSSALKIKESFSEFDAEQRFELPDSAAQEHKQCLCGEVLRGVKKPTDCKL
                     FAKACLPENPLGSCMVSSEGACAAVYAYGRVNAL"
     gene            complement(9754..9981)
                     /locus_tag="U6309_001316"
     CDS             complement(9754..9981)
                     /locus_tag="U6309_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEN5504765.1"
                     /translation="MCLALPVQIDQVLEDNKAIVNLGGITKEISTVLLEQVSEGEYVI
                     IHAGFALTKLDEREANKTLSLFAQMLKDGLK"
     gene            complement(9991..12216)
                     /gene="hypF"
                     /locus_tag="U6309_001317"
     CDS             complement(9991..12216)
                     /gene="hypF"
                     /locus_tag="U6309_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEN5504766.1"
                     /translation="MELERVQISIQGLVQGVGFRPCVYKLAKQMELTGFVQNNASGVL
                     IEIQGNYLSQFIPKLREQLPPLAAIHNLEFTSLPIVPNERAFEILDSQQGKINTVITP
                     DVCICSECLGELFDPQSRYYRYPFLNCTHCGPRFTITRNLPYDRCQTSMSQFPLCQSC
                     QSDYLNPDNRRYHAQPTACSQCGPQLALSVDKVAQRIQEGEIIALKGLGGYQLICDAR
                     NEETISKLRLRKNREAKPFALMVANVKSAEEIAEVSCQVRLVLESRERPIVLLRKKKA
                     PLPDGIAPGLNHFGIMLPSTPLHYLLFNSFLGKPDYCQWLDEYHSMVLVVTSANIGGE
                     PLIIDDKKAEQGLNSIADKIISHDREIITRTDDSVVRMINDTSVLIRRARGYVPAPLR
                     LPYEIPPTVALGGHLKNTFCITRGEEAFVSQHIGSLNNKATIEYFHESLNHLLKFLAV
                     KPERIAHDWHPDFYTSRLAQKYDIPVYGVQHHHAHLASVVAEHGIQEPVLGLVLDGYG
                     FGSDGEAWGGELLLLEKAEFKRVGHFFPIPQPGGELAAREPWRMAAGILHTLGRGDEI
                     SQRFKEQPQSPFLLQLLEKKIHCPLTTSCGRLFDAVSALLGVESLSLFEGHAAMRLES
                     MVTHPQVLNKGWTINGNVFDMRPTLQFIANTTEQQLGANLFHGTLIAGLSEWVTGICR
                     EKGIHMLLLSGGCFLNQVLAEGLINSLIKSGIKPLLPKLLPPNDGGIALGQAWIVGIK
                     E"
     gene            complement(12232..12990)
                     /gene="hypB"
                     /locus_tag="U6309_001318"
     CDS             complement(12232..12990)
                     /gene="hypB"
                     /locus_tag="U6309_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEN5504767.1"
                     /translation="MCGICGCSVEKGQTMDNEHHAAHHEHSVPHEHSVDIEQNILAQN
                     NEFAAHNRDYFLRHKILALNLMSSPGSGKTTLLSKTISDLGKEMDIAVIVGDQQTNCD
                     AQMLKSSGGYAWQINTGKVCHLDAHMISHAIQDISLKKQSLLFIENVGNLVCPASFHL
                     GEQFRIVVLSVTEGENKPLKYPDMFHSADLLLITKADLLPHVNFDLDLCKEYVRRTNP
                     SIDIITLSAVNGVGLKDWYDWLLHSFRLNVQASS"
     gene            complement(12990..13331)
                     /gene="hypA"
                     /locus_tag="U6309_001319"
     CDS             complement(12990..13331)
                     /gene="hypA"
                     /locus_tag="U6309_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEN5504768.1"
                     /translation="MHELWLCKRIVEIIKQQATGNKCRKVKKVVLEIGQLVAVDKHAL
                     NFSFKVITQGTIAQNAKLSIVEIPGEAICNSCQQIVPMKQYYDECLICGNHSLTLTKG
                     EELKVKSMVVE"
     gene            complement(13343..14020)
                     /locus_tag="U6309_001320"
     CDS             complement(13343..14020)
                     /locus_tag="U6309_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HEN5504769.1"
                     /translation="MNDSGWFLISTALLLGMRHGFDLDHLATIDAITRAVRYRPSLSR
                     MVGFLFSLGHGLVVIIVSFIIGGGTISASTPEWLNEVGNIVSVTCLLLFGFLTLWNTV
                     HYSSSAIIPTNLRSYLFRKLADNNVHPLMILLIGALFALSFDTISQIVLFSLAASALS
                     GFLFSGLLGFVFMLGMMISDGLNGFFVASLIRRADGFSLLFSRLVGFGIAFFSIGIAI
                     INCCKLL"
     gene            14166..15125
                     /locus_tag="U6309_001321"
     CDS             14166..15125
                     /locus_tag="U6309_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN5504770.1"
                     /translation="MPKTAKHKKLISCVVPVFNEEVLIAEFIAALDKTLKSIAYPYEI
                     LLIDDGSQDNTFAIIQTLRKEYSLRCIRFSRNFGKEKALSAGLDHARGDAVILLDSDF
                     QHPLELLSEFIAKWEDGYDMVYAVRQNRSDESWLKRVCAKIFYHLTSKINRINIPANA
                     GDFRLLDRKIVNALQTLPERNRFMKGLYSWVGFKQIAVPFNVHPRKSGSSQWSFYSLL
                     DLAITGITSFTAFPLRMISLGGMGVAALALIYAAWIVLSTLIFGIETPGWATIVTAIS
                     FFGGLQLFAIGVVGEYIGRVFDEVKHRPHYIIDEESSFDDHCS"
     gene            15091..15498
                     /locus_tag="U6309_001322"
     CDS             15091..15498
                     /locus_tag="U6309_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEN5504771.1"
                     /translation="MRSPVLTTTALKLSHRLIYFTGVGICAAVAHLLIVFNLVNFLNL
                     QPLLSNVIAFLIAFNVSYLGHKYFTFNQLQDEKQLSLPHFFLVAASAGLLNETLYYLL
                     LRFTTLHYIAALVFVLSIVAVYNYLFSRFWACR"
     gene            complement(15475..16287)
                     /locus_tag="U6309_001323"
     CDS             complement(15475..16287)
                     /locus_tag="U6309_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEN5504772.1"
                     /translation="MSDLKNIFLCADDFGLNHGITQGILKMARLQRLSAVSCIVNASS
                     FDVDAPDLLALKNGVRTGLHFNLTEGYFLSHPDKLCFGLRELLIRSHLGILNSRLISD
                     ELNTQLDHFIKVMGKLPDFIDGHQHIHQFPGIRRIIIDMYEERLKGKSIFIRSTYPSV
                     NMSGYQAKTKILGITGGKILSSQLKKTNIPHNPYFAGIYDFSDRTNYRSLFRQWLNNV
                     PTNTLIMCHPGEGENSDIIAEARARELNYFLSDEFMQDCEEYQVNLTTGPKS"
     gene            complement(16454..17344)
                     /locus_tag="U6309_001324"
     CDS             complement(16454..17344)
                     /locus_tag="U6309_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HEN5504773.1"
                     /translation="MPFAHLLLALLVVFVWGINFIFVKLSLEEFSPLLLCAVRFLLAS
                     VPAVFFIKPPAVPFKIIAGYGLIMFALQFALLFIGLRVGMTPGVASLLMQVQVFFSMF
                     FAIIFLGEQPQVGQIIGALIAFIGIGVVALHFDHNVSLMGFLCILAAAASWGIGNLIT
                     KKIHSVNLIAVIVWSSFVACLPMFILSLVFEGPESFVTAYEHMTWKGILSVLYIVYIS
                     TWVGYGVWNWLISRYPVGMVVPFTLLVPVVGILSSVFILGEPFYLWKLVAGLLVISGL
                     CINLLTTRLFVAKTQQPETA"
BASE COUNT         5404 a         3856 c         3029 g         5091 t
ORIGIN      
        1 tagatttccc tacctcaaaa tctggatcag gggttgctcg ctcatttgga cttaatttac
       61 aatccataaa gaatgctgac attttgttaa cagctgatac taaaccaggc tctactgttg
      121 attctgtgag agttgcagcc gctctactat aatccgaagc tgctccccat aaagattgta
      181 aaatagcata tccttttgga gatattgttt tatcactcaa ctctggcctt aaatcatctt
      241 ccttaagatt tttttccttc ataaatttat gaagctcatt cgctgcttta agatatccgc
      301 tgatgttacc cttctcaagc atgctattcg cgttgtccca caacttcttg gcattttcca
      361 gattttcaat ccttgtgaca cgttgaacac ccagttctct tattttggct acctcatcaa
      421 gcatttcttt agcatcagaa taaggcatct catcgtgttc ttttttatgc tcagcatgca
      481 actcaaatct cttgctcatc gcttgattgt atactgactc gattcgagcg atttgctctt
      541 cgggcaataa atttttttca cgtaataaac gtatagttcc tggtgctgaa agatgggctc
      601 catcctcgct gtataaatta aattcctcac gcaatcccat cagcataaaa tcgatgggac
      661 gcataatatg agttttgaca ctcactactt ctgcaccctc tttaggtgca gtaaagtctt
      721 taatggcttt attataaaac ttcttggctg aaaccatatt gctgaaagaa ggttcttgtt
      781 tgattttatc tcgtaactct tctcctaatt catagcgagg tagaacaggt tttgatgtta
      841 aaattgacat tgcagttgct tcaacatcag ctaccatccc gtcttttaac ataccaaaca
      901 aatcatcagg cgtaccaata agacggcttg gattgattcc aataggatca ggatatatct
      961 gattgcttgc agtaaaaata cgctgacata aattatttaa ggcgtcaaag accggtttta
     1021 ctttttttat atcttcgtca tttttaacaa taacaaaggc atccaaatca ctatactcag
     1081 tggcttgtgc cttggcaaga gaacctgcaa aatggacttc catatctttc ggcatctcga
     1141 caccaagcgc gtcaaaagca tcataaatca tttctttgta aatttgacgt aatccattct
     1201 cccactcctg ccatggagat acttcattgc cctcattttc tcgaattatt ttacttagat
     1261 tcgccaattt gttctgatat tcttcaaatt ttttctgagc ggttggggat agtaatggtt
     1321 tgtctcgctc atcactgtat aaactaccaa attgctcttc attaacactc attttaattc
     1381 tccccattat gctcatgtac tcgaggattc ataagtcttc agataagata gataatatct
     1441 ctcgacttgt aattaagtat atcatttggt atattatgcg atcattaagg atattgtctt
     1501 tacatataat ttacaattta cacatctaat acacaatgac aggatatctg aagtaaattt
     1561 aggaggagta acgatgcgtt cgattattac acaaatctgt aatggtgttc ttcatggaca
     1621 aacctatcag tctggttcca atgatttaga taagggtaat agtgaaatct ttgcatccag
     1681 cctgtttgta catttgaatg aacagggcaa agaaattata aagcacaagg attctgacga
     1741 taaaatagtc attggatata ccaaagatgg aatggctttt caaattgtag tggatggctt
     1801 ttatggatgc gaacgtcagg ctgttttttc atttattgat aaccatgtac tccctttaat
     1861 tgataacttc tccttagatc tcgccagata cccaaattca aaaaatgtta ccgaatcatt
     1921 cattcatact atttactctt tgcgcagcaa acatgctcca ttggcagagt ttactatgtc
     1981 tttatgcgta acttatcaaa aggatgatca actgttttgt gcaggatttg gcattggtga
     2041 caccggaata gctatcaaac gaaacgaagg aacaattgaa caattagtgt gtcatacaga
     2101 agttgatgga ttcaaagacg catttgacaa ctattcatcc accaatatcg actcggtcat
     2161 tgaaagaaat tctgtcttta atactaaagt aatgcctggt gatgagctgg tgggatacac
     2221 ttatgttcca ccggcattag aaattactga aaaagagttt gaagtagaaa ctgttgatgg
     2281 taagaaaata aacaaaagaa ttgtaagaca tctaaatctc gatcctggaa attttgatga
     2341 caaagatccc ttattttctc aattgctaca ggttgtaaaa tccaaacaaa aacagctggt
     2401 tgagcaagca aaagaaacag gacaaatcca acgttttggt gatgatttta cagtaggaag
     2461 attagtcatt cctgatcagt tactaataaa tcaattaaga atgcatgctt tatcaatcag
     2521 agtaagtgat ggattacttt catacatcaa gaatgaaaat gaaaacaaag gttttcttgg
     2581 gatatatggt ttcttcacag gggctgataa aaatatagaa aaagcaactc tttataaaaa
     2641 tctcatcgca aaataccaaa ataatccttt tacctcgcta attattcttt ctgctctagt
     2701 ctctgatagt aaaacacccg taatgactca atacttagtt gactatctgg atttcccgtc
     2761 gaaagcatca cttgctaata aaattacagc attgctttta aaagaattag aaaatccaga
     2821 tatgagagaa atactgggat ccaggttagc aactgatgtt atagaagaat tagaaactaa
     2881 aattattcgt tatattcata acccaacagg ctcggatatt cattcaacgc taaacctttg
     2941 gactgcagat aaaattaagg ctgcaactaa ctcgagtctt actatttaag tattataagt
     3001 tgattatgaa aagaaactca tacgatatga tttaacatat ccttgagttc cttttttatt
     3061 ttgataacaa ctgccctcat tgctttttca actggtttag acagcatcgc attaaaagcg
     3121 atgctatcaa tttcaatccc atataaaata atatgctctg gcagagcatt aagactatgg
     3181 cctaattgaa gagcttgagc cacccccatt tcatgggtcg ataacatatt tttaaatgca
     3241 taaatctctt tattttttaa gcgatggatt gtacctatgg cgcatcccga ttttacagca
     3301 tcaattaaaa aggcgacttt ggcggaattc atcaattcaa tcaaacgaag accaggccga
     3361 tcgtgagact ctatgcacac tgatttacta atgtattgct cttttgacaa ttctgcctta
     3421 agacaatcag cagccaaaca tcccacctga tcatcaccaa aaggtgagcc tatacctaag
     3481 atttttataa gactcatatt ctatcaattt ttaaattcag aaaatgtgta gagcatgaaa
     3541 tacacggatc ataattacga atgatcatct cactaaaata cctgatcgct tcttcttcct
     3601 gattaaggcc aaattgtatt aaagaatgct ttaaatcttc ctcaatacgc gcttgatttt
     3661 ggctagtggg aggaacaata cgcgcttgta aaacctgacc ggtttcatca aaaacataat
     3721 gctgccacag catacctctg ggagcctcgg tacaacctgc tccttctcct gcctgaggtt
     3781 tgatttcagg acgagattgc tctggcaagt catattgttc cagaatacgg atagcttcca
     3841 aaacacagta gtacatctcc acggctcgag caataatact atggcacata ttccgcgaag
     3901 gaaattgaat actcaagctt tgtaacagaa gatgaatggg tactggcaaa tgcccaaaac
     3961 aattattaat acgggccaga ggacccacta aataaggttt attttgcaat aaacagtgca
     4021 atgcattgga gtaatccaca tgatactctt tgaaatagct atcaaattct tctttgctta
     4081 tgtttaatcc acgatctgaa acaattcttc cttcattcat aggatattca gtagggtgat
     4141 aaagcgaaac actggtaaaa tcatgagaat tatcgggtaa agataggctg gcaagccaag
     4201 caattaacgc ttcgcaatcc gaaaccctat cttttgcctt gtctaatatt aatttgattt
     4261 gtgaatgaga aggcgcttta taaaaacctc caacgcaagc tcccactgga tgaacggagc
     4321 gcccccctaa caatttaatc aaatcattac caaaagcctg aatccgcaac ccgcatttca
     4381 cttcctctgg atatttcctt gccatttcag gtgctgattg aaatcctaaa aaatcaggta
     4441 aagctaaaaa atagacgtgc aagctatgac tttccagcca ctcgccacaa taaaacaggc
     4501 gacgcatatc gcgaacccaa ggagtgggac ttaggttaaa acattgttct atagcctggg
     4561 tagcactcat ttgataagca acagggcata taccacaaat acgggctaca aaatccaaca
     4621 cttcattgta acttcgacct tccagaaatt tctcaaataa tcttgggggt tcataaattt
     4681 ttaattttaa tgtgtcaatt ttgctatccc gaatgcaaag ttcaagagcc ccctctcctt
     4741 ccacccgcgc caaaattgga acattaattg aaatgtcttt acccattgct gattttaatt
     4801 cccttaaagt aatttccggc ttgattaaat gcaggtgcct gattattaat atgtaaaaac
     4861 tgcctggcaa tcgcttcttt ggttaatccg atagataaaa accactctcc caaagaattg
     4921 ggattagcat tttcttttgg cccataacaa gcataacatg ctctgcctaa agtggggcaa
     4981 agcgacccac agcctgtttg agttactggc cccatacaag gttgtttttt ggtcaccagc
     5041 acacacacat tcccttttcg tttgcactcc atgcattcag aatctctttt tattttgggg
     5101 gtcgcgttag ataataaaaa acgaatcgcc tccaatatct gcccgctatt tacaggacac
     5161 ccccataact cccaatccac tttaacgtga tgcgcaatag ccgttgaggt agttaaagtc
     5221 ttgatataat ccggtgacgc ataaacgctg gctatccact ccctggagtc tgcaaaatta
     5281 cgtaaggctt gtataccgcc cgctgtagcg caagcaccta tggtgattag gtattgactg
     5341 ttttcacgaa ttttacgtat tcgatgctct tcatcaggag ttgaaatact gccttccaca
     5401 aatgcgatat ccactttcat atccacatcc accatgcctg cctctgcaaa atgcacaatt
     5461 tcaaccaagt ctgataaagt caacaacgat tcgcccgcat ttaaaaatgc caattgacat
     5521 ccatcacaag aagtaaattt atgtacagcg atacgcggtt tcatagcatc cctcaggcac
     5581 tttaaaatcc aggttctttg aataactctt taatctggga ataagcaaag acagggccat
     5641 ctttgcatac gaataaacca ccgtattgac aatgaccgca atgaccaata ccgcactcca
     5701 tgtttctctc catactgaga tagatgtcgt tttcaagaat ccccttttgg gtaaacactt
     5761 taacagctgt attcatcatc atttcggggc cacacatcat caccacagag ttatctggtt
     5821 gtataatgat gtggtcaatc aagtcagtca catagccaac accccaaggc catttaggac
     5881 cagcttgatc tgcggctata taaacttcag tatgatctga tttttgccat ttggcatact
     5941 gctttctaaa aataaaatcc tcgctgtgtt ttactccctg caaaatactg agcttaccgt
     6001 aatgacgtcg tcttcctaaa atataattga taatggaaac agatggcgca caacccaatc
     6061 ccccggttaa aacaataatg tctttaccta tcgttttttg tagtggccag cctactccaa
     6121 aaggacctcg tattccaaga cgatcgccaa cctgaagttt ttgcatggct ttggttaccc
     6181 gccccaccgc tcgaatagtg tgactcagga aagttttctt tctgggatcc gagacaattg
     6241 aaatagccac ttcacctacc ccataaaggt acagcatatt gaattgacca ggataaaatt
     6301 gaaattgctt atgatgttct tcatcagtaa aacgcaaatg cagggtaaaa attgaagacg
     6361 actcctgcgt acgctgaaca atttcaactt ctcttggcaa gtaaggatca tagtctgatt
     6421 tattcacttt ctattttcct tacattagac tcgcccgaaa tcactgccaa ctcttcagtt
     6481 atatcaattc ccacaggaca ccaggtcaca caacgtccac atccaacaca accgctggta
     6541 tcaaattgat caaaccagct tccaaccttg tgagttagcc attgcctgta ttgttttttt
     6601 gtgtcatcac gaataatttt cccattgaga taagtatgac ctgttgtaaa acaggagtcc
     6661 cactctctat ggtgttcact gttacaacca tccaggcctg gcttttcaac ttcactatga
     6721 caaaaacagg ttgggcatac caaagtacaa ttaccacagg ataaacatcg ttcggccacg
     6781 tcatcccaac gggtatgatt taaattcgaa aacagcaaat ctcgcaaatc ccttttattg
     6841 tccagaggaa tcctcttggt ttgcaaggca gcggcttgtt tcacattatt ttgggcatca
     6901 gtacattgag cactttttgc atcatgtaaa tccagttgat taataatcct tttaccttta
     6961 tcactacctg cttccaccac aaatccatct tcaatttccg tcataagaat atcaaacgaa
     7021 ttgttcactg caggaccagt tccggcagaa acacaaaaac aattcgcaga ggaataggta
     7081 caattcactg caacaacaaa taattgctcg cgccttcttt tgtaacgagg atccggctta
     7141 ttactttcta taaaaacttt atcttgaatg ctcatcgccg ctaaatcaca agcgcgagaa
     7201 ccgataatgg caacaggtgt ttcatctggc tgatgtggtt taaagcacaa ttttccttgt
     7261 tcatcacgct caaccttcca aaccgtttcc tgtggcttaa acaatagagg ctttattcct
     7321 tgagggccat tggcaaatgc aaaagctttg cgttcgggaa ttcgttccaa tcgatacccg
     7381 cctggttctt gatgatcacg cataccccat ggtaattgct cagccctggt caggacatca
     7441 taaacaatcg caccatcacg cacctgaggc cccacacagg aatatcccgc attttgcaaa
     7501 gaatcaatta aatgttgcaa tttatcatga ggcaaaaaac tggccgtctt gtcattcatc
     7561 ctcgtcatcc tttcttgtca accattagta cttaattaac agattctggg taattgctct
     7621 ccggccagcc aatccacaat tcgtaacccg cccaattttg ttttcagctg aacaaaacag
     7681 cgatgatctt caatcacttc gccaatgatt ttagaatgta aacctaaggg gtgcgctttc
     7741 atagcaatta atagcctctc tgccttttca gaagggcaaa tcgcaattaa ttttccttca
     7801 ttagcaacat aaagaggatc aagacctaat aattcgcacg cgctagcaac ctcggtacga
     7861 atcggaattt tactttcatc aataacaaac ccgactttgg attgtgaggc caattcattc
     7921 agtgtcgttg cgagcccgcc acgcgtagga tcacgcaagc aatgaagatc tggaaccgct
     7981 ttcaccatct caccgacaag ctcatgcaag gaagctgtat cagactgcac tctggtctga
     8041 aattccaggt tattgcgatg cgccataata gctaccccat gatctcctat aaaaccactc
     8101 acaatgattt tgtcaccagg ttttgctgcg tcacctgaaa tatgcattcc ctcagggaca
     8161 atacctactc cggttgtcgt aataaaaatg ccatcgcctt ttccccgctc cacaactttt
     8221 gtatcgccag caatgatagc aacagctgct tgcctggctg catcggccat cgaataaacg
     8281 attttcctca aatcgttaag aggaaaccct tcctcaagaa taaagctggc tgtcaaatac
     8341 aaaggttttg cccctgacat agccacatca ttaaccgttc catgcactga caaagaacca
     8401 atgtccccgc caggaaaaaa aaggggggaa ataacatggg catcggttgc aatgaccata
     8461 cgtcctgaag gaacaataaa gcaagcctga tcatttttct gtgccaacca ctcgttatca
     8521 aacgcagtta aaaataattg ttcgattagc tgagccatag cacggccacc actgccatgg
     8581 gtcaaattga tagtaccttc tttaaaattc agttttggcc cagttaagtt tttagttata
     8641 aggatttcac tcataaagca ttcacccgtc cataagcata aacagcagcg caagctcctt
     8701 ctgatgaaac catacaggag cctaaaggat tttctggcag gcaagcctta gcaaacaatt
     8761 tgcaatcagt tggctttttt actcctctta atacctctcc acaaagacac tgcttatgtt
     8821 cctgtgctgc actatcaggt aattcaaaac gctgctcagc atcaaattca gaaaaagact
     8881 ctttgatttt taacgcgctg gatggaataa aacccaaacc acgccattca aaacgatccc
     8941 gtatttccag gatttcagtc atgatgtgtt gcgacaaaag attacccgta cgattcactg
     9001 cacgagtata ttgaatttct acttcacacc gcccctcatt caattgttga attaacatga
     9061 gaatagaatg caacacatcc aagggttcaa accctgatat cactatcggc ttttggtatt
     9121 ttttacacac atgctcataa ggatagcttc ctatcacgat actgacatgg gctgggccaa
     9181 caaagccgtc cagggcaact tcattggcct gtagcaaact atccatagca atgggagtta
     9241 acacatggtt acaaaataca ctgaaattag tgatgtttaa ttttttagct tgcttgatga
     9301 caaaagcagt cggcggggtt gttgtttcaa agcctatagc aaaaaataca acgtctttat
     9361 cgggattttc ctgagcgatt tttaatgcat cattcgcaga gtaaatcatc ctgacatcag
     9421 ccccgcctgc tttggcggtt aacaaacttc tttgtccagt acccggaact cgcagcatgt
     9481 cagcgtagct gcacagtatt acttgaggca tcgaggccag cgcaatagcc ttatcaattc
     9541 tcgcaacagg caaaacacaa accgggcaac cggggccatg aatcatttca acattgttgg
     9601 gaagcaaact tggaatccca taacgatgaa tagcatgagt atggcctcca caaaactcca
     9661 taaactgata atgtctttgc ggctctgctg tcgcgataat ttcattgaca atggcttgtg
     9721 cgtaagcacc gttacgaaac tcttctatgt atttcacttc aagccatcct ttagcatctg
     9781 ggcaaacaaa ctcaatgttt tgtttgcctc tctttcatcc agttttgtta aggcgaatcc
     9841 tgcatgaata atgacgtact ctccttcaga aacctgctcc aaaagaacag tggaaatttc
     9901 cttagtgatc ccacccaaat taacgattgc cttgttatct tccaaaacct gatcaatttg
     9961 cactggcaac gctaagcaca taagccaccc ttattctttt attccaacaa tccatgcttg
    10021 acctagcgca attccaccat catttggtgg aagcaatttg ggcaacaggg gtttaatacc
    10081 tgatttaatt aatgaattaa ttaagccttc cgcaagaacc tggtttaaaa aacagccgcc
    10141 actcaatagt agcatgtgaa tccctttttc tcgacaaatt cctgtaaccc actcgctaag
    10201 acctgctatt aaagtaccat gaaacagatt ggcccctaac tgttgctctg tcgtgtttgc
    10261 tataaactgt aaagtaggcc gcatgtcaaa aacattgccg tttatcgtcc agcctttgtt
    10321 taagacttgt ggatgagtca ccatgctttc caatctcatg gccgcatgac cttcaaacaa
    10381 ggagagcgat tcaacaccta aaagcgcgct gaccgcatca aacagtcgcc cacaactggt
    10441 agtcagcgga caatgaatct ttttttccaa taattgcagt aaaaatggcg attggggctg
    10501 ctctttaaac cgttgcgaaa tctcatcccc ccttcccaac gtatgcaaaa tgccagcagc
    10561 catacgccag ggttctcttg cagcaagttc gcctccgggc tgaggaatag ggaaaaaatg
    10621 tccaacgcgc ttaaattccg ccttctccag taataataat tcgccccccc aagcttcgcc
    10681 atcagaacca aacccatagc catccagaac caatcctaat acaggctcct gaattccatg
    10741 ttctgctaca acagaagcca agtgggcatg atgatgctgc accccgtaaa caggtatatc
    10801 atacttttgc gctaatctac tggtataaaa atcaggatgc caatcatgag cgattcgttc
    10861 tggtttgacc gcaagaaatt ttaataaatg attcaatgac tcatgaaaat actcaatcgt
    10921 cgctttattg tttaaactgc caatatgttg tgaaacgaaa gcttcttcgc cacgagtaat
    10981 acagaaggta tttttaagat gtcctcccaa ggccacggta gggggaattt cataaggcaa
    11041 tcttaaagga gcaggcacat agccgcgtgc tcgacgaatc aatactgaag tgtcattaat
    11101 catgcgaaca acagagtcat ctgtgcgagt aatgatttcc cgatcatgag agataatttt
    11161 atcggctata gaatttaaac cttgctcagc ctttttatca tcaataatga gcggttcacc
    11221 gcctatgttc gcactggtca ccactaatac catggaatga tactcatcca gccattggca
    11281 ataatccggc ttgccaagga acgaattaaa aagcaaataa tgtaatggag tagaaggtag
    11341 catgataccg aaatgattta agcccggagc aatcccatcc ggtaaaggtg ctttcttttt
    11401 tcttaacaag acaatcggcc tttctctgct ttccaatacc aatctcacct ggcagcttac
    11461 ctcggcaatt tcttcggcac tttttacatt ggctaccatc aaagcgaatg gtttggcctc
    11521 acgatttttt cttagccgca attttgaaat agtctcttca tttcgagcat cacaaattaa
    11581 ttgataccct cccaaaccct ttaaagcaat aatttccccc tcctgaattc tttgagccac
    11641 cttgtcgact gatagtgcga gttgcgggcc acattgacta caggctgtag gttgcgcgtg
    11701 atagcgacgg ttatcaggat ttaaataatc cgattgacac gattgacaaa gaggaaactg
    11761 gctcattgat gtttgacaac gatcatatgg cagatttctg gtaattgtga accttggacc
    11821 acagtgagta caatttaaga aaggataacg atagtatctg ctttgagggt caaataattc
    11881 gccgaggcat tcagaacaaa tgcaaacatc cggagtaatg acagtattta ttttaccctg
    11941 ctggctatca agaatttcaa aagctctttc gttcggaaca attggcagtg aagtgaactc
    12001 taggttatga attgctgcga gaggcggtaa ttgctctctt aatttcggaa taaattgtga
    12061 taaatagttg ccttgaattt ctatcaaaac acctgatgca ttattttgta caaagccagt
    12121 taattccatt tgtttggcaa gcttataaac acacggtcta aaaccaaccc cttgcactag
    12181 tccttgaatg ctaatttgta cccgttccaa ttccatgggc taccactttt tctatgaaga
    12241 agcctggaca ttcaacctaa aagaatgcaa caaccaatcg taccaatcct tcaatccaac
    12301 accattaacc gctgataggg taataatgtc tatggatgga ttcgtgcggc gtacatattc
    12361 cttacataaa tccaaatcaa aattcacatg gggtaataaa tctgctttgg ttatcaggag
    12421 caaatctgct gaatgaaaca tatcaggata tttgagcggt ttattctccc cttctgtcac
    12481 agaaagaacc actattctga attgctcgcc tagatgaaat gaagccgggc aaacgagatt
    12541 acccacattt tcgataaata aaagcgattg cttttttagg gaaatatctt gtatggcgtg
    12601 actgatcata tgggcatcca aatgacatac ttttccagta ttaatttgcc aggcatagcc
    12661 tccactggat tttagcattt gagcatcaca atttgtttgt tgatcaccta ctatgactgc
    12721 aatatccatt tccttgccaa gatcagaaat ggttttggac agtaatgttg tcttcccgga
    12781 tcccgggctt gacatcaaat tcaaagccaa aattttatgc ctgagaaaat aatcccggtt
    12841 atgagcagca aattcattgt tttgcgctaa aatattctgc tcgatatcaa ctgaatgttc
    12901 atgaggcaca gaatgctcat ggtgagcagc atgatgctca ttatccatgg tttgaccttt
    12961 ttctactgaa catccacata tcccgcacat tattccacca ccattgattt cacttttaat
    13021 tcttcacctt ttgtcagtgt taaggaatga ttgccgcaaa tcaagcactc atcataatac
    13081 tgtttcatcg gtacaatttg ttgacaagaa ttgcatattg cttcgccagg aatttctaca
    13141 atcgataatt ttgcattttg tgcaatcgtc ccttgagtta tcactttaaa gctaaaattc
    13201 aatgcgtgct tatccactgc aactaactgc cctatttcca gtactacttt ctttaccttt
    13261 cggcatttat tgccagtggc ttgttgcttg ataatctcga caatacgctt acataaccat
    13321 aattcatgca tttttagcct gtttaaagca atttgcagca attgattata gcaataccta
    13381 tgctaaaaaa agcaatgcca aatcctacta atcttgagaa aagtaatgag aacccatcag
    13441 cacgccgaat caagcttgct acaaaaaatc catttaatcc atccgatatc atcattccca
    13501 gcataaaaac aaaacccaat aacccggaaa atagaaaacc tgataaagca ctggctgcaa
    13561 gtgaaaacaa tacaatttga ctaatcgtat caaaagataa ggcaaacaac gcaccaatga
    13621 gaagaatcat aagaggatgt acattgttat cagcaagctt cctgaataaa taactcctta
    13681 agttggttgg tatgatggca gaagatgaat aatgaaccgt attccacaag gttaaaaacc
    13741 caaataacaa taaacaggta actgatacaa tatttcccac ttcatttaac cattcaggag
    13801 tactcgctga aatagtaccc ccgccaatga tgaaactcac aataataact accaaaccat
    13861 ggcctagtga aaacaaaaag ccaaccatgc gcgataaaga aggtctataa cgaaccgcgc
    13921 gagttatcgc atcgatcgtt gccagatgat ccaaatcaaa accatgacgc atgccaagca
    13981 aaagagcagt tgaaatcaaa aaccaacctg aatcgttcat tagtatgagg ccattattaa
    14041 tgttaaagac tgtggacaat ataccaatac tttcactgtt tttataaccc tcaatggact
    14101 caacttccaa gagtaggtta acatatgaag ctatctatct tttgtctggt gcattaatac
    14161 tcattatgcc taaaactgca aaacataaaa aactcatttc ttgtgttgtt cctgttttta
    14221 acgaggaagt acttattgct gaattcatcg ccgcattgga taaaactctt aagagcatcg
    14281 cttatcctta tgaaattcta ctgatagatg atggaagcca ggacaatact ttcgcaatta
    14341 ttcaaacatt aagaaaagaa tattctcttc gttgcattcg tttcagtaga aatttcggca
    14401 aggaaaaagc gctcagtgca ggattggatc atgcacgcgg agatgcggta atcttattgg
    14461 acagtgattt ccaacacccg cttgaattat taagcgaatt tatcgccaaa tgggaagatg
    14521 gttacgacat ggtttatgcc gtgagacaaa atcgttctga cgaatcgtgg ctaaaacgag
    14581 tttgcgctaa aattttttat catctcactt ccaaaataaa ccgtattaat atcccggcaa
    14641 atgcaggtga ttttagattg ctcgatagaa aaattgttaa tgcactacaa acccttcccg
    14701 aacgtaatcg ctttatgaag ggtttataca gctgggttgg ttttaaacaa atagccgtgc
    14761 catttaatgt acacccccgt aaatcgggta gttcacaatg gagtttttat tctttactgg
    14821 atttggcaat tactggcata acatcgttta ctgcttttcc tctaagaatg atttccctgg
    14881 gaggaatggg ggtagcagcc ctggcgctta tttatgccgc ctggattgta ttaagcacgc
    14941 taatctttgg cattgaaacc cctggctggg caaccatagt cactgcaatc agtttttttg
    15001 gcggattgca attatttgct atcggtgtag ttggcgaata cataggccgt gtttttgatg
    15061 aagtgaaaca cagaccccac tacattattg atgaggagtc cagttttgac gaccactgct
    15121 cttaaattat cccatcgatt aatttatttt actggagtag ggatctgcgc tgctgtcgca
    15181 catcttttga tcgtgttcaa tttagtcaat tttctaaatc ttcaaccgct gctgtctaat
    15241 gttattgctt ttttaattgc attcaatgtc agctatttag gtcacaaata tttcactttt
    15301 aatcagctac aagatgaaaa acaattaagt ttacctcatt tttttctggt ggcagcttct
    15361 gccggcctac ttaatgaaac actatattat ctgctattac gcttcacaac gttgcattat
    15421 attgctgccc tggtttttgt tttaagtatc gttgcagttt acaactatct tttttcaaga
    15481 ttttgggcct gtcgttaaat taacttggta ttcctcacaa tcttgcataa attcatcact
    15541 caaaaaataa tttagttccc tggctcttgc ctctgcaata atatcactat tctcaccttc
    15601 accgggatgg cacatgatta acgtatttgt cggaacattg tttaaccatt gcctgaataa
    15661 agatctgtag tttgttctgt cagaaaaatc gtaaatccct gcaaaataag gattatgagg
    15721 aatatttgtt ttctttaact ggctacttaa aattttaccg ccagttattc ccagaatttt
    15781 tgtttttgct tgatatcctg acatgttaac cgaagggtaa gtggaacgaa taaaaatact
    15841 ctttcccttt aatcgctctt catacatatc aataataatt cgcctaatac caggaaactg
    15901 atggatatgt tgatgaccat caataaaatc cggcaacttt cccatgactt ttatgaaatg
    15961 atccaattga gtattcaatt catctgagat taatcttgaa ttcaagatac ccaaatgcga
    16021 tctgatgagt aattctctta aaccaaaaca aagcttgtca ggatgagata gaaaataacc
    16081 ttctgtgaga ttaaaatgta agccagtgcg aaccccattt ttcaaagcca ataagtctgg
    16141 ggcatcgaca tcaaaagaac tggcattcac aatacagctt actgcggata agcgctgtaa
    16201 acgagccatt tttaaaatac cctgggtaat accatgatta aggccaaaat catcagcgca
    16261 caagaatata tttttcagat cactcattaa taacttctaa ttccaaaaaa aattgtaatg
    16321 tattactgcc cctatcctta ttttgtaata accaaaataa ttatctgatc atccatatta
    16381 taggggtgat agaaatttaa ctccctgctc cacttatgat cttaaaagga tatatagaat
    16441 cagctgggga taactaggct gtctcaggtt gttgcgtttt tgcaacaaac aggcgagttg
    16501 tcagtaaatt aatacaaagt ccactaatca ccagcaatcc ggcaaccagc ttccagagat
    16561 aaaatggttc tcccaaaata aagacagaac tcaaaatacc cacaactgga accaataaag
    16621 tgaaaggaac taccatgcca actggataac ggcttatcaa ccaattccat accccataac
    16681 caacccaagt tgaaatataa acaatataaa gcacggaaag aatacctttc caggtcatat
    16741 gttcataggc agttacaaag ctttcaggtc cttcaaaaac cagagaaagt ataaacatgg
    16801 gcaaacaggc aacaaaacta ctccaaacaa tcacagcaat cagattaaca gaatgaattt
    16861 ttttcgtaat gaggttgcca ataccccagg aagccgctgc agcaagaata cataaaaacc
    16921 ccattaaaga cacattgtgg tcgaagtgta aggccactac accaatgcct ataaatgcaa
    16981 tcaaagcccc tattatctgc ccaacttggg gttgttctcc taaaaatatt attgcaaaaa
    17041 acatactgaa aaagacttgc acttgcatga gcaaagaagc cactcctgga gtcatgccca
    17101 cacgcaaacc aataaaaagc aatgcaaatt gcaaggcaaa catgattaac ccataaccag
    17161 caataatttt aaaaggtaca gcaggaggtt tgataaaaaa cacagccggg acactggcta
    17221 aaagaaatct gacagcgcaa agaagcagcg gagaaaattc ttcaagacta agcttgacga
    17281 aaataaaatt aattccccaa acgaaaacaa ccaataaagc taacaagaga tgggcaaaag
    17341 gcatgactga agtcaacttt tataaaagtc tctttatttt
//
LOCUS       DASLOE010000048        17076 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.048, whole genome shotgun sequence.
ACCESSION   DASLOE010000048 DASLOE010000000
VERSION     DASLOE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17076
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.048"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1436
                     /gene="legL3"
                     /locus_tag="U6309_001325"
     CDS             <1..1436
                     /gene="legL3"
                     /locus_tag="U6309_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL3"
                     /protein_id="HEN5504774.1"
                     /translation="AFAIKDLDLSGPLSYEDVLFLAEYLKVTPVTDLDMSMTITKENS
                     KALWDLSQVIAKNSLLQKLTFEVQLDFDFYFKHNLPIGWLDFIDDYRLKKIAAPLHEA
                     LVNMLKNKPALNKLTIDFLAMETPFHNKHLNQLARSINKIPLNYLNLGLSVTKETQIP
                     LFKTKEPNQTLEFLILRGFNSGRNTLKYLPQTAALKMLAIDDMQFEEHDLKLLTNIMK
                     SNSSLVALILGKTNLGQVNSPDVLTWLSACPNLNSLILIDNNLSRLNVDSLCELLANN
                     LQALKKLDLSKNAYMGDDAIKIAKALTFNTHIETLVLDDNYIENEGLQAIIDLVKTNK
                     NITSLSISNTCRYTPSNEIIHSLCDLLRDPGCQLKELKFNQDITLAQYLMLQHALNSN
                     KTLEYIDFNFRNQNILEAILGKNNSGNLNENEFSSDNPKEEKAPNTLNTDPTSKSAPL
                     FFISPKSRHEENKNELSSLPSHSVHLH"
     gene            complement(1530..2648)
                     /locus_tag="U6309_001326"
     CDS             complement(1530..2648)
                     /locus_tag="U6309_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1661 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504775.1"
                     /translation="MNTVPLKPQRLLSLDVFRGMTIVLMIFVNGQAAIDPYPIFEHVD
                     WNGCTLADLVFPFFLFIVGLTSVISLKNQMERKAKTSLYSAIIERSVVLFLLGLFLNV
                     FPHPIEFDSIRIYGILQRIAVCYLISAFIYLNTSIKTQFFIFLVLLLGYWIIMTQVPV
                     PGYGANQLTKDGSWVSYFDQLFFSASHLYEKTYDPEGFLSTFTSIATTLSGVLAGSLL
                     INPCNQFKKFYLLAGVGLLFLLLGWLWNMSFPINKNLWTSSYVLWTSGLALLAFAFCY
                     LLIDRLGVKKWSVFFKIFGMNALFAFVFHVLLLKLQYAFKITTPDGSKMALISYLKDY
                     FFGGFSNHNAALLYSMCFLFLNFLVVLILYKRNVFIRI"
     gene            2809..3405
                     /locus_tag="U6309_001327"
     CDS             2809..3405
                     /locus_tag="U6309_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5504776.1"
                     /translation="MFSKKERTLLFANNLWYLSEGMLGPLFGIFCERFNSSPLDISWV
                     WSLYLISVGIVVIGLGYISDFIHKEKLLVAGYALQTIFTFCYIFITSKKQLALLQIGL
                     GISGGLSTTTWYALYARYEDKTHEGLTWGLANGLSSIYTGIALLCGGLIIAKTSFDVL
                     FIIMGCIQFIATIYVSKLIFIEHPKIIRRFRNPNLAKN"
     gene            3569..4081
                     /locus_tag="U6309_001328"
     CDS             3569..4081
                     /locus_tag="U6309_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504777.1"
                     /translation="MFESFRKCLSAISMRAPYASTPGHRNAQRANAGSEVLGTTLETF
                     DPAASIPTKLAQTLISIYSLFRFDTHVSEKLIHLLQGSIAATQMGLGIALLFTGTECE
                     EYTDADLCKAIFLLQLLYRGTLLVGWAPSEFSKDPYAEPKVVSEDKAESDAESEEENE
                     DEESRNSASV"
     gene            complement(4140..6446)
                     /gene="pbpC"
                     /locus_tag="U6309_001329"
     CDS             complement(4140..6446)
                     /gene="pbpC"
                     /locus_tag="U6309_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEN5504778.1"
                     /translation="MRKVIKYLSIILGSLFVSGFLLLFFSPKPSLLEEFNFSKAVYDD
                     NQQLLRLILSDDDKYRLYVPLSQIPKELMEATLLQEDQYFRWHKGVNPFSMVKAIWQT
                     YVVKSRRIGASTITMQVARIRFGINSKKISGKLWQIIRALQIERHYDKDKILEAYFNL
                     APYGGNIEGVGAASLIYFNKPVSKLGLPEALTLSIIPQNPVKRTSKNSELKKIRNRLF
                     ARWLNQHPQDQEKQPLFGLPLVMQTNQNLPFLAPHFVNMVLKENPGLSQSISTTLDYR
                     LQLIVERITRHYLARKKMFGVHNAAVLLVDSRTMSIKALMGSADFFNNKISGQINGVE
                     TKRSPGSTLKPFIYGLALDQGLIHPNTVLKDVPQSFNGYNPENFDYDFMGPIKAKDAL
                     ILSRNIPAIELSNQLTNPTLHKLLEQAHVTQLRSESYYGLSLNLGGVELTMKELVGLY
                     AMLINDGVWYPINSLKGVPRQKGRRLLSTEASFLVFDMLKNTPMKESNNEGSSQLPIA
                     WKTGTSSGYRDAWTVGVFGPYVLAVWIGNFDNQANPAFIGKEIAAPLFFELIDAIKHE
                     RGPIARVEKYPEHMHLEKIEVCKASGMLPSRYCTDTEWTWFIPGKSPIKTDTIFREVA
                     INGKTGLRTCHIDENTRFEVYEFWPSDLLRIFRRAGIQRHTPPFFEPGCALSGNSGIS
                     PQITSPQAGVSYIVHANTQSNKIIPLTAVTDAGIAHLYWFINESFVAETKSDEPYLWQ
                     AKPGKYVVRVVDDRGLSDARDIIIQMVS"
     gene            6631..12396
                     /locus_tag="U6309_001330"
     CDS             6631..12396
                     /locus_tag="U6309_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HEN5504779.1"
                     /translation="MNKNPFSLIMNVLCSIFSTLFGKIKWNHPTWLVYLKHKSKNSPQ
                     IFWGTTLLFIVLLGAGAYGAYWYNNLPKPTYTTASITIPDITPNEENLVPNNLIIDFG
                     IRSNEFIPQSVAPINLIGKEIDEGIEMAPQIAGKWSWNSDSQLIFIPAEDWPAGQKYT
                     IHFAKNFFAPNANIESYDLSFTTKPFQATISEFKFYQDPVNAETRHAVATIEFNYPVN
                     PKSLEKNTSAVFQTIKDGKADLTAESVPFSYTYDNNKRIAYLHSANIKIEDVSRYLLL
                     TLNKNVSSSTDSAILGQSISKNLLIPDASNFLKIVSTSANIVRNDKDRPEQVLSIETS
                     LGVNESEFNKSVHVYLLPQNYPATAAEPEKSNYEWRNPGEVTNNILSLSTPLPKEVIP
                     SEQNYTTLHGFKFKAQTPRYLYIKIDKGMKGFGGFKLSNDYTAIVPVPEYPKEISFLH
                     KGSLLALSGEKKLSVLIRGVPAVKYEFARVLPDNVNQLVTQTQGDFSNPYFINPSFNQ
                     QNISQIFSEIQQYDSTDLTRQQYTSLDLGKYLASEANASGPQGLFLLQATGWDVANQY
                     ALDVKASRLILITDLGMVVKDNNDGSHDVFISSITQGLPVAGATVTILGKNGLSILSR
                     VTDEQGRANFPTLKDFIEDREPTVYLANLNNDVSFIPFNNYNRQLNFSKFDIGGIYTN
                     DQDLQNLTAYLFSDRGIYRPGDTVHVGMIVKRAFAEPQPSGLPLQATVVDARGTTIKD
                     QKVTLNDTGYMELDFTTSANSPTGQYMVYLYLVKDNHPQNLLGSTSVRISEFQPDRMR
                     ITSSFEPKPFDGWSSPTDLKAKVDLWNLYGAPAANRRVSGKILLTPQRVQFDNYPDYI
                     FADPLYDPKKPPKVFTENLAEATTNENGQAEFSLNLERFEKATYQLTFFAEGFEPGSG
                     RSVTTQTKTLVSPLPYFIGYKADGDLHFIKQNSVRNVNYIAINPQLGKEDVKNLKIQL
                     ISLRPVTTLVKKADGTYQYQSTIQSTVINTNPFEIKESGINYSLPTNTIGDYSLNVLD
                     KDDSILSQLQFSVVGTSQAPLAKNAELSIKLNKEEYQANEDIEIQITAPYTGSGLITI
                     ERDKVYAVHWFKTETQNSVQTIHIPADFQGNGYVNVAFIRDWESPELFISPLSYSVAP
                     FNVNHDKHDIRIGLKTPELARPGESFTIDYHTDKPGKIIVFAVDEGILQVANYTTPDP
                     LSFFFQKRALEVLTQQTVDQILPKFIQDREISAVGGDEGEAALASRLNPFKRKTDLPV
                     VYWSGILDADSTDRQLSYQIPDYFNGSIRVIAVAVANDSMGSREVSAEVRGDFIISPN
                     VPTFVAPGDEFEISTTVANNIKDSGEHAQVAVELTASPELEIIGSAKESLEISEGNEQ
                     TVYFKLRAKSLLGSARIAITAKSGDKTSKMDSTLSVRPANPFTTYINSGKSQDSQKSL
                     EVIQTLYPEHRKVEATISTSPLVLIFGLQRYLDNFPYGCTEQLTSKALPWLVMNSLPW
                     FSSESGPIHEKINTTIQMLSQRQMSNGGFSYWPGLSDNSANSFASVYAMHFLTEARAQ
                     GFNVPGEMFYNGINYLKELASRNPTDADSARIQAYAIYILTRNELVTTNYLANLQLYL
                     QQDSSKNWLKDITAVYIAATYQLLKSYSEANQLIGQYQPPTNNAFTTDFYDRNIADAQ
                     YLYLVAKHFPNLLPQVSDNILMNLVEAINNDEINTVLSGFSSLALGAYPQNESVDLSD
                     KLSITEVLANNQQRNIPTDQNNYQKLNIDETVKTIRFNNPDKQTFFYQLIQSGFDKII
                     PTEPLKQGIEIYREYRDEKGNVIDSVVLGEEIEVHLQVRSLDNNYLSNIAIEDLLPGG
                     FEVVRDSVKTDNMDYADVREDRVNFFGWVDLTAKELVYKIKATNVGKYIVPPAYAEAM
                     YNPNTKARGSSSVITVSREED"
     gene            complement(12644..14005)
                     /locus_tag="U6309_001331"
     CDS             complement(12644..14005)
                     /locus_tag="U6309_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1666 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504780.1"
                     /translation="MGKKAYIIGMGPAGLASALALLAKGYSVELIDRRSEDDVFSRKQ
                     GVFLKDDTIRDFFALSQLASKGYSLEFKDNFICKLIRPIDESNLKLSAEDQLDLAFFE
                     LIEKERTVIPIAELQRYQYSKLKYIYAKGGFTFQDEEDPYTVKFNNAEQQLIFHLGDT
                     QIAKVDAENQELTLDHNGSIQNHSFDLLVDASGKTSKSFTQLWNSQNPEFAIGYEKLD
                     NPDHNAFGVMYLSINNPPLAMIANPVLSPIEGTSFIPLDKITPLKAMGWTHDYPPLIY
                     LKYSKKNGAVYLTGEIPESILKDNNKKELKQWFDLVLQLLFNNTNFKTSAPGLASVFK
                     TDIEIADKFALPLPKGGVFCLVGDALMSANFLLGCGISNALDDANQLPDMVDNQFSQR
                     EAEAYRNLRYLDYKDDWLSFREHIALRLKSLQLEKQLDLSRQELELKQRELENIQQQC
                     SFY"
     gene            complement(14208..15599)
                     /locus_tag="U6309_001332"
     CDS             complement(14208..15599)
                     /locus_tag="U6309_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1667 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504781.1"
                     /translation="MEYKEVIPMIRFFIICFIFLVSHQAYANPYWDRYKVPRDSHYPN
                     LKFHYINPDSLKIVQENVGIDVLRIQTDWFEETDSYQVGTVIIESSGTPALLSRSIHK
                     PKWGSYLGVLKSRFGGDTAYYDAIGTGKEYRKLARAINLRFPVPSHDMIFELYAENPQ
                     TGLMEKVVEKNIIASELTRDNRHFNDLEVRQLLSAVKTPSLRVNIYAEGYTQSEKLKF
                     WNRAIKTVQALQSQQFPGIDYMDFYAVFNPSHKRLGVAEDLGLPVPEFDSFLGLYYPY
                     WDNFGRWYHIVYPTRENKFRQGLASAPYDYPIVLVNNSQYWGVGNYKSHTAIPADNST
                     YFTYILIHELGHFFGLNEEYEGGGRTELEFAPEMKEPWSQNITFLTTPSYSGLKWNEL
                     VNPQMSIPTPYSDWQSTPPQYGAYRGGYGDSPSHQGASHKPGLNCVMESHASFCDICQ
                     KGIMDVIRFDLGT"
     gene            complement(15746..16570)
                     /locus_tag="U6309_001333"
     CDS             complement(15746..16570)
                     /locus_tag="U6309_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504782.1"
                     /translation="MKKIAIILSLFPFLICCGLANATVSQKRFPGESYQDAMKRIINS
                     KPQNELKMLIARGTPYSKKVSLQKIDYSTVPKVSSYDELTNMFNLIRDTRFLYSPDKP
                     DFQRRISWLYPDDGCFARAALSRIKLDTEHFVIPAKIFAFGDLEMQTPYSSEGTVSWW
                     YHVSAVVNYMGTIYVLDPAAKPEGPMLIDDWYNKIGIIDKMEAVVCNPYTYDPFDRCY
                     DATNKTDNKALNDQLKYLDKEWVRIESLGFDPSVLLGDNPPWIGDMGNSTLLSYKS"
     gene            16851..>17076
                     /locus_tag="U6309_001334"
     CDS             16851..>17076
                     /locus_tag="U6309_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504783.1"
                     /translation="MASDNPFSGLREKMLNLYYQAEKDLFREPVFRNQFQDAKLDDVI
                     ISRAGVKFLWGKISEDQRAHLQGLFLAYLED"
BASE COUNT         5600 a         3363 c         2998 g         5115 t
ORIGIN      
        1 gggcattcgc catcaaagat ttggatttat caggccctct atcttatgaa gatgtattat
       61 ttttagctga atatctaaaa gtcacacccg taacagatct tgacatgtca atgaccatca
      121 ccaaggaaaa ctctaaagcg ctatgggatt taagtcaggt tattgctaaa aattcactgc
      181 tacaaaaact aacatttgaa gtgcaactgg acttcgattt ctattttaaa cataacctgc
      241 caattggttg gttagatttt atagacgatt atcggttaaa aaaaatagcg gcgccactcc
      301 atgaggcatt ggttaacatg ctaaaaaaca aacctgctct aaataagtta actatagact
      361 ttttagcgat ggaaactcct tttcacaata aacatctcaa ccagttagca cgatctatca
      421 ataaaatccc gcttaattat ctgaatttgg gcctttcagt gaccaaagaa acacaaatac
      481 cgctatttaa gacgaaagaa ccgaatcaaa ctcttgagtt cctaatcctt cgtggtttta
      541 attcaggtag aaataccttg aaatatttac cccaaacagc agccttaaaa atgttagcta
      601 ttgatgacat gcaattcgaa gaacatgatt tgaaattgct gactaatatt atgaaatcca
      661 actcgtcact ggttgcttta atccttggta aaactaatct tggacaagtc aattcgccag
      721 acgtactcac ctggctcagt gcatgcccca atttaaatag tcttatactc attgacaata
      781 atctaagcag gcttaatgtg gatagtttat gcgaattatt agcgaataat ctccaggctt
      841 tgaaaaaact ggatctgtcc aaaaatgctt atatgggtga cgacgcgata aagattgcca
      901 aagcgttaac tttcaatact catattgaaa cattggtgct ggatgataac tatattgaaa
      961 atgaggggct acaagccatc atcgatctgg taaaaaccaa taaaaatata acgtctctat
     1021 ccatttcaaa cacctgtcga tatacacctt ctaacgaaat aattcacagt ctatgtgatt
     1081 tattacgtga tcctggatgt caattaaaag aactaaaatt taaccaggat attactttgg
     1141 cacagtacct tatgttgcaa catgccctaa actctaacaa aacactagaa tacattgatt
     1201 tcaattttag aaatcagaat attcttgagg caattcttgg gaaaaataat tccggtaatt
     1261 tgaatgaaaa tgagttttca agtgacaatc ctaaagaaga aaaagcccca aatactttga
     1321 atacggatcc gactagcaaa agtgctccat tattttttat ttccccaaag agtcggcacg
     1381 aagagaataa aaatgaactt tcttctttac cgagtcatag tgtccatctc cattagggac
     1441 aacacacatt acactctatt ataatttctc actggcgatg tacacagttg gcgctgagca
     1501 atctgcgagg cccaacctac cttacctctt tatattcgaa taaatacatt acgtttatat
     1561 aaaatcaaaa caaccagaaa attcaggaaa agaaaacaca tagaatacag caatgctgca
     1621 ttgtgattac tgaatcctcc aaaaaaataa tctttgagat aggatatcaa tgccatttta
     1681 gagccatcag gtgttgttat tttaaaagca tattgtagtt ttaaaagaag gacatgaaat
     1741 acaaaagcaa ataatgcatt catgccaaat attttaaaaa aaacggacca ttttttaacc
     1801 ccaagtctat caatcagcaa atagcaaaaa gcaaaggcaa gcagagccag gccgctggtc
     1861 cagagtacgt aagaactagt ccataaattt ttattaatag gaaaactcat attccataac
     1921 catcccaata acaaaaatag caagccaacc cctgctaata gataaaattt cttgaattga
     1981 ttgcatggat tgattagtaa actaccagcc aggacacctg ataaagtagt ggcaatagat
     2041 gtaaaagtac ttaaaaaacc ttcgggatca tacgtttttt catacaagtg ggaggctgaa
     2101 aaaaataact ggtcaaagta tgaaacccaa cttccgtctt ttgttaattg atttgctcca
     2161 tatcctggga caggtacctg agtcataata atccaatatc ctaataaaag aacaagaaaa
     2221 ataaaaaatt gagttttaat tgaagtattc agataaatga aagcggaaat taaataacaa
     2281 acagcgattc gctgcagaat cccatatatc cttatgctat caaattcaat aggatgggga
     2341 aaaacattca aaaataatcc taaaagaaat aaaacaacac ttctctctat gatggcagaa
     2401 tataatgatg tttttgcttt tcgttccatt tggtttttca ggcttataac actcgttaag
     2461 ccgacgataa acaagaaaaa tgggaaaacc aaatccgcca aagtacaacc attccagtcc
     2521 acatgctcaa aaatcggata gggatcaata gctgcctgac cattaacgaa aatcatcaat
     2581 acaatggtca tcccgcgaaa tacatcaagg gataaaaggc gttggggttt taagggaaca
     2641 gtattcatgc gctatttaaa atggatttat aaaaaactga gccccattat cttaccacga
     2701 gtggcaaatt gtaatatcaa aaataaaaat taaatttcta aaatctatat gaaaatctct
     2761 tttaaacttg tatgttagtc atgattgact taacttgaag ttgaattaat gtttagtaaa
     2821 aaagaacgta cattattatt tgccaataat ttgtggtact tgtcagaagg aatgttaggc
     2881 ccgctcttcg gtattttttg cgaaaggttc aactcgtctc ccttagacat atcatgggta
     2941 tggtctttgt atttaatttc agttggaatt gtagtaattg gcttgggtta tatttcagat
     3001 tttatccata aagaaaaatt gctggttgca gggtatgcac tccaaaccat atttaccttt
     3061 tgttacatct ttataacaag taaaaagcaa ctggctttat tgcaaattgg ccttggaata
     3121 tcaggtggac tctctactac aacctggtat gctctgtatg cccgctatga agataaaacg
     3181 catgaaggac ttacttgggg tttggccaat gggttgtcaa gtatttacac tgggatcgcc
     3241 ttgttatgtg gtggtctaat tattgccaaa acatcatttg atgttttgtt tatcatcatg
     3301 ggatgtattc aatttatagc aactatttat gtcagtaagc tcatctttat cgaacacccc
     3361 aaaattatca ggcgttttag aaatcctaat ttggctaaaa attaatttta aaatctgaaa
     3421 gcgatgttga attgtcaaat caaatgaata ttttttgatg tagattgatg ctaaattcat
     3481 caagaaatac tggaatcaaa ataacttgtc tgttaatgtc cgcttttatt aaagatatca
     3541 cgtattaaat tgttataagg atatgattat gtttgaatct tttcgaaaat gtttgtctgc
     3601 catttctatg cgagctccgt atgcctcgac acctggacat cgcaacgcgc aacgcgctaa
     3661 tgccggatcc gaagtattgg gtacaacgct tgaaacattt gatcctgccg catcaattcc
     3721 aacaaaactc gctcaaactt taatctcaat ttattcccta ttcagatttg atactcatgt
     3781 ttctgaaaaa ctgatccatc ttttgcaagg tagtatagcg gcaacacaaa tgggtcttgg
     3841 cattgctttg ctttttactg gaacagagtg tgaggaatat acggatgctg atctatgcaa
     3901 ggcaatattt ctattacaat tactatacag aggaaccttg ctagtgggct gggctcctag
     3961 tgaattttcc aaggatccct atgctgagcc taaagttgta tcagaagaca aagcggaatc
     4021 tgatgctgaa tctgaagaag agaacgaaga tgaagagtca agaaactcgg cctcagttta
     4081 attctaaatc ccttgctccg attaggacaa gggatcactt atacatcatc aaatatgcgt
     4141 caactaacca tctgaataat aatatcccgc gcatcagata aaccacggtc atctaccacc
     4201 cttaccacat atttaccagg tttcgcctgc cagagataag gttcatctga ttttgtttca
     4261 gctacaaaac tttcattgat aaaccagtat aaatgtgcaa ttccagcatc tgttaccgca
     4321 gttaatggga ttatcttgtt tgattgggta ttagcatgga caatataact tacccctgcc
     4381 tggggagatg taatttgggg gctaatacca ctatttcccg acaaagcaca acctggttca
     4441 aaaaatggtg gggtatggcg ttgaatccct gccctcctga atattcttaa taaatcggag
     4501 ggccaaaact catacacttc aaaacgagta ttttcatcga tgtgacaagt acgaagcccc
     4561 gtcttaccgt taattgccac ctctcgaaaa attgtatccg ttttaatagg agatttacct
     4621 ggaataaacc aggtccattc tgtatccgta caatatcgac taggaagcat acctgaggct
     4681 ttgcatactt ctattttttc caaatgcatg tgttcagggt atttttcaac tcgagctatt
     4741 ggtcctcttt catgttttat tgcatcaatc aattcaaaaa ataaaggcgc agcaatttct
     4801 ttacctataa aagccggatt tgcctgatta tcaaaatttc ctatccaaac tgccaataca
     4861 taaggaccaa aaactccaac tgtccaggca tctctgtatc ctgaggaagt tcctgttttc
     4921 caggcaattg gtaattgtga gctgccctca ttattcgact ctttcattgg agtatttttt
     4981 agcatatcga aaactaaaaa gcttgcttct gtgctaagca agcgtcgacc tttttgtcgc
     5041 ggaacccctt ttaatgaatt gattggatac cagaccccat cattaatcag catagcatat
     5101 aaaccgacta actctttcat agtgagttca acaccaccca aattaagaga caagccataa
     5161 tatgattcgg aacgcaactg agttacatgc gcctgttcaa gtaatttatg taatgttgga
     5221 ttagttaatt gattggaaag ctcgatggcc gggatattac gactaagtat caaagcatct
     5281 tttgctttga tgggtcccat aaaatcataa tcgaagtttt ccggattata accattaaaa
     5341 ctctgtggta catcctttaa aactgtattg gggtgaatta aaccttgatc aagggctaat
     5401 ccataaataa atggtttcag agttgagcca ggtgaccttt tggtttctac accattgatt
     5461 tgtccactta tcttgttatt aaaaaaatca gcagatccca tcaacgcttt aatactcata
     5521 gtacggctat ccacaagtaa taccgccgcg ttgtgaacac caaacatttt ttttcttgct
     5581 aaataatgcc ttgtaattcg ctcaactatt aactgaagcc tgtaatccaa agttgttgaa
     5641 atactttgtg ataaaccagg attttccttc agtaccatat ttacaaaatg aggagccagg
     5701 aagggtaggt tttgattggt ttgcataact aaaggcagtc caaacagagg ctgcttctcc
     5761 tggtcctgcg gatgttgatt cagccatctg gcaaataaac gatttcttat tttttttaat
     5821 tcactgtttt ttgatgtcct ttttactgga ttttgaggga taatgcttaa agttagtgcc
     5881 tctggtaaac caagtttact aacaggctta ttaaaataaa ttaagctcgc cgcccctact
     5941 ccctctatat ttccaccata aggcgccaaa ttgaaataag cctcaagaat tttatcttta
     6001 tcataatgac gctctatttg taacgcccta ataatttgcc ataattttcc agaaattttc
     6061 ttggagttaa ttccaaaacg aattcgtgcc acctgcattg tgattgttga agccccgatt
     6121 ctgcgcgatt taaccacata agtttgccaa atggctttca ccatggaaaa agggttcact
     6181 cctttatgcc agcgaaaata ctgatcttct tgtaaaagtg tggcctccat taactccttg
     6241 ggtatttgag ataatggtac ataaagtctg tatttgtcat cgtcacttaa aatcaatctt
     6301 aataattgtt gattatcatc atataccgct ttagaaaaat tgaattcttc taataaagaa
     6361 ggcttgggag aaaaaaataa taataaaaac ccactgacaa atagcgaacc cagaattatg
     6421 ctaagatatt ttattacttt tctcattgcc cacaacaggg atgatataaa ggttaaatta
     6481 tattcaaatt aatattgaaa tgcattagaa ttggcaaagg atagactaat aattataaat
     6541 taataattta ttgcgggggg tgtatgcttt accgcaccct cattaccaga tcctaaaata
     6601 aacttagacc aaccaggact ccatattacc atgaacaaaa acccattctc cctaattatg
     6661 aatgtattgt gttcaatatt ttcaactcta ttcggaaaaa taaaatggaa tcacccaacc
     6721 tggttagttt atttaaaaca caaatcaaaa aattctcccc aaatattttg gggaacaact
     6781 ctattattta tcgtgttatt gggcgcagga gcatatggtg cttattggta taataatttg
     6841 ccaaaaccaa cttatactac tgcatccatc acaataccag acatcacacc caatgaggaa
     6901 aaccttgttc ccaacaatct cattattgat ttcggcatta gaagtaatga atttatccca
     6961 caatcagttg cccccattaa tttaattggc aaagaaattg atgaagggat agaaatggct
     7021 ccgcaaattg cgggtaaatg gagctggaat agtgatagcc aattaatttt tattcccgct
     7081 gaagattggc ctgcagggca aaagtatact attcattttg caaaaaattt ctttgcaccc
     7141 aatgctaata ttgaaagtta tgatttatcc tttacaacaa agccctttca ggccacaatc
     7201 agcgagttca aattttacca agatcccgtc aatgcagaaa caaggcacgc tgtagccact
     7261 attgagttca attatccagt aaatccaaaa agtcttgaaa aaaacacctc cgccgtattc
     7321 caaaccatta aagatggaaa agcggatctc accgcagaat ccgttccttt ttcttatact
     7381 tatgataata ataaacgaat tgcctatctt cactcggcaa atatcaaaat agaagatgta
     7441 tcgcgttatt tacttttaac tttgaataaa aacgttagtt cttcaactga ttcagcaatt
     7501 cttggacaat caattagcaa aaatttgctg atacccgatg caagtaactt cctaaaaata
     7561 gtttcaacat cggccaatat agttcgtaat gataaagaca ggcccgaaca ggtacttagt
     7621 attgagacca gtttgggtgt taatgaaagt gaatttaata aatcagtaca tgtctatttg
     7681 ctccctcaga attatcctgc taccgctgca gaaccagaaa aatccaatta cgaatggcga
     7741 aatccaggtg aagtaacgaa taacattttg tctctgtcga ctccattacc caaagaggta
     7801 atcccttctg agcaaaatta tactacgttg catggcttca aatttaaagc tcaaactccc
     7861 agatacctgt atatcaagat tgataaagga atgaaaggtt ttggtggttt taaattaagc
     7921 aatgattaca cggctatcgt gccagtacca gaatacccca aagaaatcag tttcttgcat
     7981 aagggttctt tgttagcact gagtggcgag aaaaaactgt cagttctgat acgcggagtg
     8041 cctgcagtta agtatgaatt cgcgcgtgtg ttgcctgata atgtcaatca gctggttacg
     8101 caaacccaag gcgatttcag caatccttat tttattaatc cctcttttaa tcaacaaaac
     8161 atcagtcaaa ttttctcgga aatccagcaa tatgactcca ctgatttaac cagacagcaa
     8221 tacacatccc tggatctggg aaaatattta gcatctgaag ccaatgcctc aggaccgcaa
     8281 ggattgttct tattacaagc aacaggttgg gatgtagcta atcaatatgc tctcgatgtt
     8341 aaggccagtc gtttgatatt gattacggat ttggggatgg tggtaaaaga caataatgac
     8401 gggagtcatg atgtctttat tagctccatt acacaaggac tcccagttgc aggggcaaca
     8461 gttacgattt taggtaaaaa tggcttgtcc attttatctc gtgttacaga tgaacaaggg
     8521 cgagcaaatt ttcctactct gaaagatttt attgaagaca gagaaccaac cgtgtatttg
     8581 gcaaacctga ataatgatgt gtcttttatt ccattcaata actacaacag gcaattgaat
     8641 ttttcaaaat ttgatattgg aggaatctat accaatgatc aggatttgca gaacttaaca
     8701 gcttatttat tttctgatag aggaatatat aggcccggag acacagtgca tgttggaatg
     8761 attgtaaaac gagcctttgc agaaccacaa ccttctggtc tgccattaca agccactgta
     8821 gtagatgcca ggggaactac tatcaaagac caaaaagtga cattaaatga caccggttat
     8881 atggagcttg attttactac cagtgccaat tctcccactg gtcagtatat ggtttattta
     8941 tacttggtaa aagataacca cccgcaaaat cttctcggtt caaccagtgt ccgtatttcc
     9001 gaattccaac ccgacagaat gcgcattact tccagctttg aacccaaacc ttttgatggc
     9061 tggagctcac ccactgatct gaaagcaaag gtagatcttt ggaacttgta tggtgcccca
     9121 gctgccaata gaagggtaag cggcaaaatt ttattaactc ctcagcgagt gcaatttgac
     9181 aactatcctg attatatttt tgcggatcca ctgtatgatc cgaaaaagcc cccaaaagtt
     9241 tttacagaaa acctggcaga agcaacaact aacgagaatg ggcaggctga atttagcctg
     9301 aatcttgaac gttttgaaaa ggcaacttat caattaactt tttttgctga aggctttgag
     9361 cctggtagtg ggagaagtgt tactacacaa actaaaacat tggtcagccc tttaccctat
     9421 ttcattggct ataaagctga tggcgacttg cattttatta aacagaacag cgtcagaaat
     9481 gttaattaca ttgcaattaa tccacagttg ggtaaagaag acgtaaaaaa tttaaagata
     9541 caattaattt ccctgcgccc cgtcacaact ctggttaaaa aagctgacgg cacttatcaa
     9601 tatcaatcca caatacaatc aacagtcatc aataccaatc cattcgaaat taaagaatct
     9661 ggcattaact acagcctgcc aacgaatact attggtgatt actccctcaa cgtattagat
     9721 aaggatgata gtattcttag ccagctgcaa tttagcgtag ttggcaccag ccaggctcct
     9781 ctcgctaaaa atgccgaatt atctatcaaa cttaataaag aagaatatca agccaatgaa
     9841 gatatagaaa tccaaatcac tgctccttat accggctcag gcttgatcac tattgagaga
     9901 gacaaagtct atgctgtaca ttggtttaag acggaaaccc aaaattctgt acaaaccatc
     9961 cacatacctg ctgattttca gggtaatgga tatgtgaatg tggcgtttat tcgtgattgg
    10021 gaatctcctg aactcttcat tagcccgtta agttatagcg ttgctccatt caatgtgaat
    10081 catgataagc acgatataag aattggacta aaaacacctg aattagcccg tcccggagaa
    10141 tcttttacga ttgattatca cacagacaaa cccggtaaga tcatcgtatt tgcagtggat
    10201 gaaggaatat tgcaggttgc caattatact acacccgatc cacttagctt ctttttccag
    10261 aaacgagcat tagaagtatt aacccaacaa acagttgatc aaattctacc caagtttatc
    10321 caggatagag aaatatctgc tgttggaggt gatgaaggcg aagcggcttt agcaagtcgc
    10381 ctcaatccat tcaaaagaaa gaccgactta ccagttgttt actggtccgg tattcttgac
    10441 gcagacagta ctgacaggca attatcttat caaatacctg attattttaa tgggtccatt
    10501 cgggtgatcg ctgtggcagt cgctaatgac tctatgggat ctcgcgaagt ttcagcagag
    10561 gttcgcggtg attttattat aagtcccaat gtgcctactt tcgttgcacc cggagatgaa
    10621 tttgaaatat caaccactgt ggcaaataat attaaagatt caggagaaca cgctcaagtt
    10681 gcagtagaac ttaccgcatc accagagcta gaaataatcg gtagtgcgaa agagtctctg
    10741 gaaatcagcg aaggcaatga gcaaactgtg tattttaaat tgcgcgctaa atcactgttg
    10801 ggatcagcaa ggattgctat cactgccaaa tcaggagata aaacaagcaa gatggatagc
    10861 acgctcagcg tcaggcctgc caatcctttc acaacatata ttaatagtgg caaatcacaa
    10921 gattctcaaa aatcgttaga ggttatccag actttatatc ccgagcatcg caaggtagaa
    10981 gcgactatat cgaccagtcc attagttcta atctttggat tacaaagata tttggataat
    11041 ttcccatatg gttgtacgga gcaattgaca agcaaggccc tgccctggct ggttatgaac
    11101 agtttaccct ggttcagttc ggaaagtggc ccaattcacg aaaaaattaa taccacaatt
    11161 caaatgttaa gccaaagaca aatgtctaat ggtgggttta gctattggcc tggtctaagt
    11221 gacaattcag cgaatagttt tgcatcagtt tatgccatgc atttcttaac tgaagcaaga
    11281 gcacaaggat tcaacgtccc cggagagatg ttctacaatg gcattaatta ccttaaagaa
    11341 ttggctagtc gcaatcccac agatgcagac tcagcaagaa ttcaagccta cgccatctat
    11401 attttaaccc gtaatgaatt agtcactaca aattatttag ccaatttgca attgtattta
    11461 cagcaagatt cttcaaaaaa ctggctaaaa gacatcacag cagtttacat tgcagccacc
    11521 tatcaattat taaaaagtta tagtgaagcg aatcaattga ttggccaata tcaaccacca
    11581 acaaataatg catttacgac tgatttttat gatcgcaata tcgcagatgc gcaatatctc
    11641 tatcttgttg caaaacattt tcctaattta ctgcctcaag taagcgacaa tatactcatg
    11701 aatttggtag aagcgataaa taatgatgaa atcaatacag ttttatcagg attttctagc
    11761 ttggctttgg gcgcttaccc acaaaatgaa tcagtagatc tatctgataa gctgtccata
    11821 acagaagtac tggcaaacaa tcagcagaga aacatcccta ccgatcaaaa taattatcaa
    11881 aaattaaata ttgatgaaac tgtcaaaaca atccgcttta acaatcctga caaacaaaca
    11941 ttcttttatc agcttattca gtcagggttt gataaaataa taccgacaga acctttaaaa
    12001 caaggcatag aaatttacag agaatatcgg gatgagaaag gcaatgtcat tgattcagtg
    12061 gttttgggtg aagaaattga agtacacctg caagtccggt cattagataa taattatctg
    12121 agcaatattg ccattgaaga tctcttgcca ggcgggtttg aggtcgttag agactcagta
    12181 aaaacggaca atatggatta tgccgatgtc cgggaagaca gagttaactt cttcggctgg
    12241 gtagatttaa ctgcaaaaga attagtttat aaaataaaag cgactaatgt tggtaaatac
    12301 atagttccac cagcctacgc agaagccatg tataacccaa atacgaaggc aagaggttct
    12361 tcatcagtca ttacagtgtc cagagaagaa gattaattgc tggcaagcaa agtcagattt
    12421 tgattcttga ctttgcttac taataaatat taattgccct catatttgct catgccgaca
    12481 aatgaaggag atacttatca tagcctgagg atgagataag gctctttcgt gaggaagcaa
    12541 acactattgg atcttagaga atagattcct cagcccggca tagtaatgac ttaagttttg
    12601 cattatatag taaagtatgg ttttgtcctg tcgcagggag gatctaataa aatgaacatt
    12661 gttgctggat attctccaac tctcgttgct tcaattccag ttcctgccta cttaaatcca
    12721 actgcttttc aagctgtaga gactttagcc tgagagcaat atgctcccta aaagacaacc
    12781 aatcatcttt ataatctaaa tagcgaagat tacgataggc ttcagcttcc ctttggctaa
    12841 attggttatc aaccatatct ggtaactgat tagcatcatc caaagcattg ctaatgccac
    12901 aacccaataa aaaattagca gacatcaaag catctccaac caagcaaaac acacccccct
    12961 taggaagggg caaagcgaat ttatcagcaa tttcaatatc tgttttaaaa acagaagcta
    13021 aaccaggggc gctcgtttta aaattcgtat tgttaaataa caattgcaat accaagtcaa
    13081 accattgttt taactctttt ttattattat ccttgagaat cgattcagga atttctccag
    13141 tgagataaac agctccattc ttttttgaat atttcaaata aattaaaggt ggataatcat
    13201 gcgtccagcc catggccttt aacggagtaa tcttgtctaa agggatgaag ctggtcccct
    13261 caattggact taacaccgga ttagctatca ttgccaaagg cggattattg atcgacagat
    13321 acatcacacc aaaagcattg tgatcaggat tatctaactt ttcataacca attgcgaact
    13381 ctggattttg cgaattccac aactgagtaa atgattttga tgtttttccg gatgcgtcaa
    13441 ctagcaaatc aaaactgtga ttttgtatgc taccattatg atccaaggtt aactcctgat
    13501 tctcagcatc aaccttggca atttgcgtgt ctcccaagtg aaaaatgagc tgctgctctg
    13561 cattattgaa ttttacagta taaggatctt cttcgtcctg aaaagtaaag ccacctttgg
    13621 cataaatata tttcaattta ctatactgat aacgctgcaa ctctgcgata ggaattacag
    13681 tgcgctcttt ttcaattaat tcaaagaaag ctaaatccaa ttgatcttct gcactcaatt
    13741 tcaagttgga ttcatcaatg ggacggatta atttacagat aaaattatct ttgaattcca
    13801 aggaataccc tttgcttgcc agttgactta gagcaaaaaa atctctgatc gtatcatcct
    13861 ttaagaatac cccttgtttg cgactaaaaa catcatcttc ggatcggcgg tcaattaatt
    13921 caacggaata ccctttagct aataatgcga gagcactagc caagccagcc ggccccatgc
    13981 caatgatata tgctttcttt cccataagtt ggactctact acttaaaata aatgacaaat
    14041 tttagcattt aattgttaag aaattattag gtgagcattt ctattgccaa tgccccgctg
    14101 tgcaggacaa aactcaggta gactctcttt ttgtatagga agagtagcat gttgattatt
    14161 aatttttcta aactctgatc tgggggttcc aacctgaaac ccctaattta ggttcccaaa
    14221 tcgaaacgaa ttacatccat aattcctttt tgacaaatat cgcaaaatga tgcgtgagat
    14281 tccataacac aattcaagcc tggtttatga cttgcaccct gatgagatgg tgaatctcca
    14341 tagccgccac gatatgcccc atattgcgga ggggttgact gccagtcact atagggagta
    14401 ggaatagaca tttgtggatt gactaattca ttccatttta atcccgaata gcttggagta
    14461 gtcagaaaag tgatgttttg tgaccacggt tccttcatct caggggcaaa ttccaactca
    14521 gtccgtccac caccctcata ttcttcattg agtccaaaaa aatgacccaa ttcatgtatc
    14581 agaatgtagg taaaataagt tgaattatca gcaggtattg cggtgtgtga tttgtaatta
    14641 cctacacccc agtactgact gttattaacc aatactattg gataatcata aggtgctgac
    14701 gccaatccct gacgaaactt gttctctcgt gtaggatata ctatatgata ccaacgtcca
    14761 aaattatccc agtatggata atataaaccc agaaaagaat caaattctgg tactggtaaa
    14821 cctaaatctt cagcgactcc caaacgctta tgagatggat taaagactgc ataaaaatcc
    14881 atataatcga taccaggaaa ctgttgactt tgtaatgcct gcacagtttt aatcgctcga
    14941 ttccaaaatt tcaacttctc actttgagta tacccttcag catatatatt cactcttaaa
    15001 gagggagttt tcactgcgga taatagttgc ctcacttcca aatcattgaa atgacgatta
    15061 tctcttgtta attcactagc aatgatattt ttctcaacaa ccttttccat taaacctgtt
    15121 tgcgggtttt ctgcataaag ttcaaagatc atatcatggg aaggaacggg aaaacgcaaa
    15181 ttaatagccc gtgccagctt gcgatattcc ttaccagtac caatcgcatc ataatatgcc
    15241 gtatcaccac cgaatctgct ttttaaaacc cccagataac tcccccattt gggcttgtga
    15301 atacttcgtg ataataatgc cggtgttcca gaggactcta ttattactgt accaacctga
    15361 taagaatctg tttcttcaaa ccaatcggtt tggattctta atacatcaat ccctacattt
    15421 tcctgcacaa tcttcaaaga atcaggattg atgtaatgaa actttaaatt tgggtaatga
    15481 ctgtctctgg gcactttata gcggtcccaa tagggattag cgtatgcctg atgggaaacc
    15541 aaaaaaataa agcaaatgat aaaaaatctt atcatgggta tgacctcctt gtattccaga
    15601 actactatcc catactaact tgtagatttt cctacaaatg ttaaccgagt ttaacgaata
    15661 ctcttttatc tatcgaactt gatatttcgc cagattgaac gaaagcgtat taatttttgg
    15721 gtatcgtaac aacgactcac aaaaatcatg atttataaga caaaagagta gaattaccca
    15781 tgtcacctat ccatggagga ttatcaccta ataaaacaga cggatcaaaa cctaatgatt
    15841 cgattctaac ccactcttta tccaaatatt tcaattgatc atttaacgct ttgttatctg
    15901 ttttatttgt tgcatcatag caacgatcaa aaggatcgta ggtataggga ttgcaaacta
    15961 ccgcctccat tttatcgatt atcccaattt tgttgtacca atcatcaatc aacattggac
    16021 cttctggttt ggcagcggga tcgagcacat aaatggtacc catgtagttg acaacagctg
    16081 acacatgata ccaccaactt actgttccct cagaagagta tggtgtttgc atttccaaat
    16141 caccaaaagc aaaaattttt gctggaataa caaaatgctc agtatccagt ttaattctgg
    16201 atagtgctgc tctggcaaag caaccatcat caggataaag ccaactgatt cttctttgaa
    16261 aatcaggttt atcaggtgaa tacaaaaaac gagtatctcg tatcaggtta aacatgttcg
    16321 tcaactcgtc atagctgcta accttcggca cggtggagta atctattttc tgtaaagaga
    16381 ctttctttga ataaggagtt cccctggcaa ttaacatctt cagttcattt tgtggtttgc
    16441 tatttatgat acgtttcatt gcatcctgat aagattctcc tggaaaccgt ttttgtgaaa
    16501 cagttgcgtt tgccaagccg caacaaatca aaaaaggaaa taatgataaa ataatggcta
    16561 tttttttcat agcaatcctt gagtatttat aatattggtt gatattttac caatgtttgt
    16621 cgtccttgac attctttata attagcatga actgattgaa aagactagtg tattagtcat
    16681 atggactatc agtagtcaaa acatatcaag tcaaaaacat aatgacttat atttcttatc
    16741 agtgtaagca ttcaaaatgc tgaatttaat tgaactgacc ttaaaatttg tttctcataa
    16801 taatgatcta taatttagac atacatcaat ttatagatta gaggctagtt atggctagtg
    16861 ataacccttt ttcaggctta agagaaaaaa tgttaaatct ttattatcaa gcagaaaagg
    16921 acctgttccg tgagcctgta tttagaaatc aatttcagga tgctaagctt gatgatgtta
    16981 ttatttctcg agcaggtgta aaatttctat ggggtaaaat ttccgaagac cagcgcgcac
    17041 atttacaagg tttattttta gcttatttag aagatc
//
LOCUS       DASLOE010000049        17074 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.049, whole genome shotgun sequence.
ACCESSION   DASLOE010000049 DASLOE010000000
VERSION     DASLOE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 17074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17074
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.049"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..212)
                     /locus_tag="U6309_001335"
     CDS             complement(<1..212)
                     /locus_tag="U6309_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN5504784.1"
                     /translation="MKSRLSVLSLALITVCSVDSIRNLPAAALAGNQLFYYFALALLF
                     FLLPCAIVAVWFSRQSEQGVYGWVKQG"
     gene            complement(212..979)
                     /locus_tag="U6309_001336"
     CDS             complement(212..979)
                     /locus_tag="U6309_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase"
                     /protein_id="HEN5504785.1"
                     /translation="MFKNAIVRTPSSTMINGLTTSADLGKPNYELALIQHQNYIKALS
                     TCGLEVTVLPDLEQFPDACFVEDVSLLTEQFAVLTCPGAESRRDEVQEIEPSIQAFFK
                     DRIFRISPPGRLEAGDVLRIDNHFFIGLSERTNKEGAEQLIYLLNQHGYTASVIQLKK
                     FLHLKTGVSYLNNDYVLVSGELINHQAFGHLKQIVVSPEEAYAANCIMINETVLLPKG
                     CPKITHRLSELGFSIIELDMSEFRKLDGGLSCLSLRF"
     gene            1162..2085
                     /locus_tag="U6309_001337"
     CDS             1162..2085
                     /locus_tag="U6309_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504786.1"
                     /translation="MTSNKCDIVLLTQKDFIDPANITPYIQNVLTEDRLLTEALEQKG
                     LKVKRTHWDNRQYNWSETRYALFRATWDYFHRYDEFHLWLNNCSKKIQFINPISIIQW
                     NIDKHYLNDMQQKGINIPPTLFLKRGKLNILNNLMKESGWKKAILKPAIAGGARHTYL
                     FDQDSADHYQDVFESLINNESMLLQEFQENIVNKGEVSFMVFGGRYSHSILKKAKSGD
                     FRVQDDFGGTVHPYEASQEEREFVENVIAQCDELPVYARVDVMWDNNNKLCLSELEMI
                     EPELWFRESSQAASAMADAVCEHISGVSEYK"
     gene            2127..3800
                     /locus_tag="U6309_001338"
     CDS             2127..3800
                     /locus_tag="U6309_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEN5504787.1"
                     /translation="MKTRIVSLLVVSMASANLQAASENIIWQAGDNNLKKYNFSILQD
                     KKGFMSITNKITPLNYQLKPVDHEGNRDSNDIRYQVYYRNIPVWGHELILHKASKSKI
                     FLTGVDVSEIEKDVHQTDGKLSAADIESQITKYNNNSIVYKNIKKIIYLDSHKKAYLA
                     YHLSMYANDPLHFVSAPNYIIDANSGEILKSWDDLTHKKVGQGLGGNVFILPYRSGLF
                     QHGDSMKDIPSLGKFEVTIKGKNCVVESPEVRVINAAYTDLDKRSFPVLSIVEIYKNI
                     PAFSYPCSKESQYININDGDTSPANYSFSSVNDAMYFAGVTLEMYKEYYGVEKPLGDD
                     LPLRAYTHIKNFDNAFAVPSIKIKGLYLMHQQIVIGDGDTQLTAPAQSTLSHELSHNF
                     TRLHSNLVYNGQSGGINEAFSDMASIAMQDYLRKDFPWYWDGEDWSIGREATISGEPI
                     RYMDYPTKDGHSIEHFGDYHDDLDVHLTSGIFNRAFYLLAHQANWSVRKAFQVMVDAN
                     IKYWTSGTSFEAASCGVIQAAIDRQYNKQDVINAFKEVGVICPVIGLIN"
     gene            3995..5113
                     /locus_tag="U6309_001339"
     CDS             3995..5113
                     /locus_tag="U6309_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1654 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504788.1"
                     /translation="MMDEVKRIHARQKIASAKELEKRYCIKEAITEYLRAIDLFLQIS
                     NKTPEDINAIVNAHYDLATLYFNRRDYISAGRHYEAAINHLIETPLKDDSYRLLTELY
                     IDLSDACYELMNQSAGDEAMANALKAFGLIQNKTFEEQQIGDPVANFKQFHIFYEKKL
                     STESYLNSAKFMNHEYLLGEGQVARQQEQALFEQFENISISEIQQIDRSLENMLSQLS
                     LAAEPPTSNSVSGVDRVIFNPIFVNVTPSDSSYRGMAMQVLNLAKSYIQNQKISDAIA
                     TYQQAIKILNTIKSPIESDLQIVQELTQHIRYLQNKPSSTKDQFSSWYKEAQAPVIAT
                     ASEMGFFNYRPQEAFQVSDSFHSPNEMDVEYCDSNLRP"
     gene            5526..6941
                     /locus_tag="U6309_001340"
     CDS             5526..6941
                     /locus_tag="U6309_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEN5504789.1"
                     /translation="MNKGMNAFVLLIAVIAGFGGFLFGFDSSVIADVKDQVISQLALT
                     DWQWAQVVSSSLLGCILGIPISGLVADRLSRRSLLKVVALGFILGSSLCALAPGFVSI
                     LLGRFIIGICIGIASYIAPLFIAEIAPPHQRGTLVLINGLTITFGQAIAYLIGYFLHD
                     YSLMSWRYLFWIGSLPALVLFSGMYFVPHSPRWIMMKYGADATLKTLKQIRPVGYNIQ
                     QELTEISNNLVNPAPAYIQMFKKPIVFVLLLGVGLGLFQQFSGINALMYYGPVVFESF
                     GFSPVKNAILATFFLGLVNFIFTVVTLFYVDKLGRRFLLISGTLIASISLYFVSLLSC
                     NSLFVNKFWILGGLSFYVMGYCISLGSLFWVLISEIYPLSVRGLAMSIATVFQWGANF
                     VVSLLFLPIYQFSGQTVTFAMFATFCLLACWFIYYFVPETTSVSLEKLEDNLSSGKKI
                     RDIGQPVSKNIKIAKFNLIMD"
     gene            6944..7948
                     /gene="iolG"
                     /locus_tag="U6309_001341"
     CDS             6944..7948
                     /gene="iolG"
                     /locus_tag="U6309_001341"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEN5504790.1"
                     /translation="MKKKICRIGIVGAGRIGRLHAENILFHLPQFELVAIADPQIDRN
                     WAEKLGIAIVSTQSEDVLCHPDLDAILIASPSHLHLEQIITASIHEKAIFCEKPIGLN
                     EEEINKTLNIIKNNNTLLQLGFNRRFDPSFANIQKRVQFGEIGTPHILRITSRDPACP
                     SKEYCATSGGIFMDMSIHDFDMARFLMQSEVVEVYATGAVLINPDFEQFDDVDTAIIQ
                     LRFANGAFGVIDNSRQAIYGYDQRIEVFGSAGMLLAENQLEHTVSRYSANGTDGAKPQ
                     YFFLERYHQAYLAELNAFYSAWNDNQISPVSGLDGLQALRIAKAAKQSLETHLPVVLS
                     "
     gene            7956..9842
                     /gene="iolC"
                     /locus_tag="U6309_001342"
     CDS             7956..9842
                     /gene="iolC"
                     /locus_tag="U6309_001342"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEN5504791.1"
                     /translation="MKQFPGFYFDKNRAIDLICMGRVAVDLYAEQIGLDLKDVASFKK
                     YLGGCAGNIAVGAARLGLKSQMFSCVGSDELGQFLKEELEREGVNIELLSETDNHLTG
                     LVLLGIKPPSNFPLLFYRNDCADMQIKSDQISFTDLKNAKALLITGTGLSTESMKNTT
                     LEVVHLARKAETTIVFDLDYRPVLWRLTVIGNGESRYCQNEYVTQVYQQVLPLCDLIV
                     GTEEEICIAGGSNDIEVSLINIRQRTDAPIVVKLGEKGARVHFGADKGLLQAKSFPVE
                     VLNVLGAGDGFMSGLLAGLLQGKSWEQAVTYANACGALVVTRHGCAPAIPYKEELNYF
                     IQEYDHDPEIWNSSQLTQLHEKLSKNQKTQLLIKNPCGFADGLNSIVTMQDSPTAMSF
                     SSLKLNKGQSHQFSSSYEFAALLMTGKVVFQYGSYSQEVERTDYFSQSPYVLHCSKNE
                     VSTVIALTDCEILLMETENENLFSPILFDASNMLELDNRGEGILENTSYRLVRTVFDK
                     RNRPESNLVVGEIITFQGRWSSYPSHYHQQPEIYHYRFSEPQGYAFGENGKEVMRIEH
                     YDTYQIANNQEHAHCAAPGYALYTLWFIRHLNNNAYTLPTFTKDHEWTRTSRANLRAW
                     QQKQ"
     gene            9881..11752
                     /gene="iolD"
                     /locus_tag="U6309_001343"
     CDS             9881..11752
                     /gene="iolD"
                     /locus_tag="U6309_001343"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEN5504792.1"
                     /translation="MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTG
                     LGEALEYDAQGLIYYQGHNEQGMAHAATAYAKQKNRLGIMACTSSIGPGATNMITAAA
                     TATTNRIPLLLLPGDVFSCRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQ
                     LMTACMHAMRVLTDPVETGTVTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAML
                     AEAIRHIRKAKQPLIIAGGGVHYSLASETLSAFALKHRIPVAETQAGKSSLAAKHPMN
                     VGGIGVTGSEIANSLAAQADVILVIGSRLQDFTTASKWAFKNQDCQIIHLNVSRLDAM
                     KMNGVMLKGDAKTGLEQLGKELADYQTSLSYQQLIRHYQNEWAQELDKLTQSYSTEQA
                     GLHQTTVLGLLNQYVTEEDVIISAAGSLPGDLHRIWQSKKAKDYHLEYAYSCMGYEVA
                     AGLGVRIAKENQEGEVYVLVGDGSFVMMHSELLTAIQERKKITILIFDNHGFQCIRNL
                     QEGNGSLGFGNEFRYREPTTNALNGDYLPIDFCKYAEGLGAATYFVKSYEQFRTTLDS
                     AKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEVSQSESVSKAYEMMQKQIETVRQY"
     gene            11755..12651
                     /gene="iolE"
                     /locus_tag="U6309_001344"
     CDS             11755..12651
                     /gene="iolE"
                     /locus_tag="U6309_001344"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HEN5504793.1"
                     /translation="MGLMMSIQLGIAPINWCNDDDPNLGKNISFEQCINEMALAGYSG
                     TELGNKYPRDATTLKAALEQRGLQLSSSWFSTYFTEANAFEKTVSRFLEHLSFMRALG
                     ASYINVCECGHAIQGTDLPILSAHIPQFTQEQWSLLIQGLHTLGRIAYDFNMKIVYHY
                     HAGTGVFWEKDIDFLMINTSPKLVSLLLDTGHAVFSGIDPVNLIDKYGDRILYVHLKD
                     IRPDVLRRVEMERLSFMDAVRRGVFTVPGDGAIDFIPIWAGLQKHRYNGWMIVEAEQD
                     PEKAHPLEYAKKAYDYLHSFIH"
     gene            12773..13030
                     /locus_tag="U6309_001345"
     CDS             12773..13030
                     /locus_tag="U6309_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEN5504794.1"
                     /translation="MSMKDKLIQSTISAFLVLIASNTAIAEDLSKEKCYGVVKKGMND
                     CSTATSDCAGSSTKDSQKDAFILLPKGICEKLVGGSLDMGK"
     gene            13058..13651
                     /locus_tag="U6309_001346"
     CDS             13058..13651
                     /locus_tag="U6309_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEN5504795.1"
                     /translation="MKRIYRHFIAPILAVFALSSYVYAEPQTLTLDNQHTYVLWKVKH
                     LGFSTQAGKWYASGQLVLDKDNPQQSKVNVTIKVDDIVTGIPELDKHLKGKLFFDTKQ
                     FPTATFVSDRVEVTGKNKAKVYGMLTLHGVTKPIILNVILNKAGINLLNDRETAGFSA
                     TTSLKRSDYGIKALIPEVGDDVEIEIEAEAYLDKKYG"
     gene            13655..14185
                     /locus_tag="U6309_001347"
     CDS             13655..14185
                     /locus_tag="U6309_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEN5504796.1"
                     /translation="MQLKNTSTSFGLVAIFFHWIMAALIIGLLVVGLYMVRIPISLEK
                     LKLYGWHKEYGFLVLFLAFFRLIWRLTNQLPELAIPLLEKIAARSMHWAFYFFMFAMP
                     ISGWLITSAAGLPASFFGLFVLPDLVAPDENRRILFQLIHEWLGYALIMAIILHAAAA
                     LKHHFINKDDILRRML"
     gene            14185..14760
                     /locus_tag="U6309_001348"
     CDS             14185..14760
                     /locus_tag="U6309_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEN5504797.1"
                     /translation="MNVYIKSSLWVLIFFVNTLIYASKLPEWEIVPAQSEITFTGTQN
                     GAPVTGRFKKFTGQIFADPSNYKTGSIDITIDMNSIAAPFDDIVTTLASPDWFNVKAF
                     PNAEFKASKFNQLDDKTYEAEGILTIRDKSAPVTLKFVVDQISNDQALVEGSSTIKRS
                     TFGVGQGEWESTDEIQNEVIVRFKITATRKK"
     gene            14907..16931
                     /locus_tag="U6309_001349"
     CDS             14907..16931
                     /locus_tag="U6309_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HEN5504798.1"
                     /translation="MPIPLLNFKHFQNDNSIFRSTTHHNHMVSRGTFPGNPKTWFAKV
                     CSDPASARNEVLAQEFFRLIIPHQPETLIAKDEAQNNYYVYSEEVEGFKNLPYGQGKS
                     FEDGTFTGLGQAVFVSVFLQEVDLKNGNIGLDKDGRVIKIDGDQCIVSMLGVDQRFDL
                     TPKVIDSLPRPVDFATNNWLDYKSSYFSLQRILQPTITNNEVFRSEVNQAMLKICLIP
                     DEYIERFVSVYIPDGHKPDKQGYIDLIKSRRDVLRLSAVKNESFRNYLSEPAAQDDAR
                     HFMEHMRSFQSGGEPVLASQQERSQLTIDLALQNLKLETAFIQQSEKVTTVKNAVEKE
                     AFADQENELAMVFRFQMEMKKQAQLSDSSFIGIISRSSQASAEKTAYQNFLNKTVNPV
                     MEKCFAALVRNDLKQLEQVLAEFPNNEKWSAFNLQESGVAKSQMDAVKRLIEGRVFIR
                     GKILPVLDQCKDAIDKKDIPAALQALSALPPDKDMSRTASISPGLKGQIIEMKQDVTE
                     TLNAIAQQSAIATPVIDDAKRVESGYDPLLVEITKQIEALENEHCEDLPKLEMHLTTV
                     NDCLDTVEFLISEHIKQHGASEEPLELSGLEELRSRLQKIKKTDENALNTYPKKNAEI
                     TQTEAALSQVLPQTSNNTNPAKRTIKSDYDVDSENKVTKIGEDRPGFKPE"
BASE COUNT         5408 a         2854 c         3561 g         5251 t
ORIGIN      
        1 ccctgtttta cccacccata cacaccttgt tctgattgcc tggagaacca aactgcaaca
       61 atggcacaag ggagaagaaa gaatagcaag gccaatgcaa agtaataaaa cagctggttc
      121 ccagctaaag cggctgctgg aaggtttcta atactatcaa ccgagcaaac agtgattaag
      181 gcaaggctta aaacagataa gcggcttttc attaaaatct caacgacaag cagcttaaac
      241 caccatccaa ctttctaaat tcgctcatat ccaattcaat aattgaaaat cccagttcag
      301 ataaacgatg agttatttta ggacaccctt tgggtaataa cacagtctca ttaatcataa
      361 tacaattagc tgcgtacgct tcttcaggac ttactactat ttgttttaaa tgaccaaatg
      421 cttgatgatt gattaattct ccactaacca atacgtaatc attattcagg taactcacac
      481 ctgttttaag atgcagaaat ttttttagct gaattactga ggctgtatag ccatgctgat
      541 tcagtaaata aattagttgc tcagccccct ctttgttagt tctttccgag agaccaataa
      601 aaaaatgatt gtctattcta agcacatctc cagcctcaag tctgccaggc gggcttattc
      661 tgaaaatcct gtctttaaag aatgcttgaa tgctgggttc aatttcctga acttcatctc
      721 gcctggattc tgcgccaggg catgtcaaga cagcaaattg ttcagttaat aatgacacgt
      781 cttctacaaa acaagcatcc gggaattgtt ctagatctgg aagcactgtc acttcaagac
      841 cacatgttga tagtgctttg atataatttt gatgctgtat taatgcaagc tcataattgg
      901 gtttccccaa atcagccgat gtggttaatc cattgatcat tgtagaagat ggtgttctta
      961 ctatagcatt tttaaacatt catcatgtcc ttaaataagt gatttccaat acacacacag
     1021 ccagagatta aacaaagagt cttcaaaatg caatcattgt taatttgatt aaattagctg
     1081 gttgatttgc gatggggggt atgtagattt agtttaaaaa gataagtatt cttgtatcat
     1141 ttgatacgga ttgtttctat catgacctct aacaagtgcg atattgtctt actaacacag
     1201 aaggatttta tagacccggc caatattact ccctacattc aaaatgtgct aacagaagat
     1261 cgattattaa ccgaagctct ggagcaaaag ggattaaaag ttaaacgaac tcattgggac
     1321 aatcgacaat ataattggtc agagactaga tatgcactgt ttcgtgccac ctgggattat
     1381 tttcatcgtt atgacgagtt tcatctctgg ctaaataatt gctcaaaaaa aattcaattt
     1441 ataaatccga tttctattat tcagtggaat attgataaac attatttgaa tgatatgcag
     1501 caaaaaggaa ttaatatccc ccctacttta tttttaaaaa gggggaagct caatatctta
     1561 aataatttaa tgaaggaatc gggatggaag aaagcgatat taaagcctgc aattgctggc
     1621 ggagcacgcc atacttattt atttgatcaa gactccgcgg atcattatca agatgtattt
     1681 gaatcattaa ttaacaatga gtcgatgtta ctccaggaat ttcaggagaa cattgtaaat
     1741 aaaggagaag tctcttttat ggtgtttggg ggtagatata gccattctat actaaaaaaa
     1801 gcaaaatctg gcgattttag agtacaggat gatttcggtg ggactgtgca tccttatgag
     1861 gcatctcaag aagaaaggga gtttgttgag aatgtcatag ctcaatgcga tgagttaccc
     1921 gtgtatgcac gtgttgatgt catgtgggat aacaataata aattgtgttt gagtgagttg
     1981 gaaatgatag aacctgaact ttggttcagg gaaagcagtc aagccgcgtc tgctatggct
     2041 gatgcagtat gcgaacacat atcaggggtc agtgaatata aataaacgct gtaagacaca
     2101 acaatagtta aagacaggga aaaaatatga aaacacggat agtttcatta ttagttgttt
     2161 ctatggcaag tgctaattta caagctgctt cagaaaatat tatttggcag gcgggcgata
     2221 acaatttaaa aaaatacaat tttagtatct tgcaagataa aaaaggattt atgtcaatta
     2281 ctaataaaat cacgccgtta aattatcagt tgaaaccagt tgatcatgag ggcaatcgtg
     2341 attctaacga tattcgttac caggtttatt atagaaatat tcctgtttgg ggtcatgagt
     2401 taatccttca taaggccagc aagtcaaaaa tttttctgac cggtgtcgat gtgtctgaaa
     2461 ttgaaaagga tgttcatcag actgatggga aattaagtgc tgctgatatt gaaagccaga
     2521 taacaaaata taataacaat tcgatcgttt acaaaaatat aaaaaaaatc atttatctgg
     2581 attctcataa aaaagcttat ctagcttatc acttatcaat gtatgcaaat gatccattac
     2641 attttgtctc cgctccgaat tatattatcg atgccaattc cggtgagatt ttaaaaagct
     2701 gggatgattt aacacataag aaagttggtc agggcttggg tggtaatgta ttcattcttc
     2761 cgtatcggtc gggattgttt caacatgggg attcaatgaa ggatattcct tctctcggca
     2821 aatttgaggt aacgatcaaa ggaaaaaatt gtgttgtaga aagccctgag gtaagggtca
     2881 ttaatgctgc gtatactgat ttggacaaga gatccttccc cgttctaagc attgtggaaa
     2941 tctataaaaa tataccagca ttttcctacc cctgtagcaa agaatcacaa tatatcaata
     3001 ttaatgatgg agatacatca cctgcaaact acagtttttc ttcagttaat gatgctatgt
     3061 attttgccgg agtcacactg gaaatgtaca aagagtatta tggcgttgaa aagcctttag
     3121 gtgacgactt gcctttgcga gcctatacgc atattaaaaa ttttgataat gcctttgctg
     3181 tgccttctat taaaataaaa ggattgtact taatgcatca acaaattgtg attggtgatg
     3241 gcgatactca attaacagca ccggcacaga gcactttatc gcatgagttg tctcataatt
     3301 ttaccagatt gcattctaac ctggtctata atgggcaatc cggaggaatc aatgaagcat
     3361 tctcggatat ggcatcgatt gctatgcagg attacttgcg taaagatttt ccatggtatt
     3421 gggatggtga agattggtct atagggcgtg aggctactat aagtggtgag ccaattcgtt
     3481 acatggacta tccaacgaaa gatggtcatt ctattgagca tttcggtgat tatcatgatg
     3541 acttggatgt tcatctgacc agcgggattt ttaacagggc tttttatctt ttagcccatc
     3601 aagcgaactg gtcagtgaga aaggcatttc aagtgatggt agatgccaat ataaaatatt
     3661 ggacatcagg gacttcattt gaagcagcct cttgtggtgt tatccaagcg gcaattgaca
     3721 ggcaatataa taagcaggat gtcatcaatg cttttaaaga ggtaggagtg atttgtcctg
     3781 ttataggcct cataaattaa atacagtaat tcacgtgact tcgatacgga ttgctgataa
     3841 aaagagataa tgatattaat tcaaccttcc agttcataac caattctttt taatttcaga
     3901 caattaagga aattcctatt tgttttaagt gggttggatt attttacaat acgttgtctt
     3961 tatatatgga tctgaattcg cattaaagga gtcaatgatg gatgaagtaa agagaataca
     4021 tgccagacag aaaatcgcct ctgccaaaga gcttgaaaag agatattgca taaaagaggc
     4081 aattaccgag tatctaagag caattgattt atttttacaa atttccaata aaactcctga
     4141 ggatattaat gcaatagtta atgcacatta tgatctggcg accctatatt ttaatagaag
     4201 agattatata agcgccggta gacattatga agcagcgata aatcacctca tagaaacccc
     4261 actaaaagat gactcttacc gacttctaac agagttatat attgatttat ctgatgcgtg
     4321 ttatgagctg atgaatcaat ctgctgggga tgaggcaatg gctaatgcac taaaagcatt
     4381 tggattgatt caaaataaaa catttgaaga acaacaaatt ggggatcctg ttgctaattt
     4441 caaacaattt catatttttt atgaaaaaaa actatcaaca gagtcctatt taaactccgc
     4501 aaaatttatg aatcatgaat atctgctcgg tgaggggcaa gttgcaaggc agcaggagca
     4561 agcgcttttt gagcagtttg aaaatatatc gattagtgaa atacaacaaa ttgatcgtag
     4621 cttggaaaat atgttatcgc aattgtcttt ggcagcagaa cccccaacct ctaattcggt
     4681 ctctggagtt gatcgagtta tttttaatcc gatttttgta aatgtaacgc ctagtgatag
     4741 ctcttatcgc ggcatggcaa tgcaagttct aaatctagcc aaatcttata tacaaaatca
     4801 aaagatttca gatgcaatag ctacttatca acaagccatt aaaatcctga atacaattaa
     4861 atcacctata gaaagtgatc tgcaaattgt tcaggagcta acgcaacata taagatactt
     4921 acaaaataaa cctagctcta caaaagatca attttcatcc tggtataaag aagctcaagc
     4981 tcctgtaatt gccactgctt cagagatggg attttttaat tacaggccac aagaggcttt
     5041 tcaggtatcg gattcatttc atagccctaa tgaaatggat gtggaatact gtgatagcaa
     5101 tttgcgccca taagtacgtt gctagttgag cgacgataat tattttgtgt tcaaacggca
     5161 gataggcagg attttagggt ctttgccttt tgcagcaata ttgcaaaaac taccaaattt
     5221 agaaacctct gcatttcaag tataattaat tcttgtaact tgtgattgca gtgcagcaag
     5281 aatttgcgtg acatagggtg ttgcaccctt tgttgcatga attatctact tatggtgatt
     5341 taaattggaa cgttggctaa gtgtgtcctt ttcccgccca aaagtgtctg tttaatcagc
     5401 aaatctccat tattggggta aagtattatt ttatcttcaa taatttacac tttgatattt
     5461 ttctcgcaac ctgaccgtga taacatcatg cacgaacaga attttcatta gggacagata
     5521 gttacatgaa caagggaatg aacgcatttg tactcctgat cgcggtaatt gccggatttg
     5581 gcggtttttt attcggattt gattccagcg tcatagctga tgtgaaagat caggttatct
     5641 cccaattggc actaacagac tggcaatggg cacaagttgt cagcagtagt ttattgggat
     5701 gtatattggg aatacccata agtggtcttg tcgcagatag gttaagcaga cgatccttat
     5761 taaaagtagt agctttaggc tttatactgg ggagtagttt gtgtgctttg gctcccggct
     5821 tcgtttcgat attactcgga cgatttatta tcggcatttg cataggtatt gcctcctata
     5881 ttgctccttt attcattgca gaaatcgcgc cgcctcatca gaggggaact ttagtattga
     5941 ttaatggact aaccatcacg tttggtcagg ctatcgccta tttaattggc tattttctcc
     6001 atgactattc tttaatgagt tggagatatt tattttggat agggagtcta cctgctctcg
     6061 ttttattttc gggaatgtat tttgtccctc attcaccccg ctggataatg atgaaatatg
     6121 gagctgatgc tactttaaag accttaaagc aaattagacc agttggatat aatattcagc
     6181 aggaattgac tgaaattagt aataatttag ttaatcctgc accagcctat atccagatgt
     6241 ttaaaaaacc aattgttttt gttctcttat taggagttgg tttaggacta ttccagcaat
     6301 tttccgggat caatgcctta atgtattatg gacctgttgt ctttgaatca tttgggtttt
     6361 ctccagttaa aaatgcaata ctcgctacct tttttttggg tctggtcaat tttattttta
     6421 cagtggttac ccttttttat gttgataaat taggcagaag gtttttgtta atcagtggta
     6481 ctttgatcgc atcaattagc ttatattttg ttagcttact atcgtgcaac tctttatttg
     6541 tcaataaatt ctggatttta ggcggtttat cattttatgt gatggggtat tgtattagcc
     6601 tggggtctct gttctgggtt ttaatatccg aaatataccc cttatcagtg cgtggtttgg
     6661 caatgagcat tgccacagtg tttcaatggg gcgctaattt tgttgtatca cttttgtttc
     6721 ttcccattta tcaattctca ggtcaaacag ttacctttgc tatgttcgct accttttgct
     6781 tgctggcatg ctggtttatt tattattttg tcccggaaac cacatcggta tcgttggaaa
     6841 aactggaaga caatttaagc tctggaaaaa aaataagaga tataggtcaa ccagtatcga
     6901 aaaatataaa aatagcaaaa tttaatttaa ttatggatta atcatgaaaa agaaaatatg
     6961 ccgaattggg atagtaggtg cgggaagaat tggtagactg cacgcagaaa atattctatt
     7021 tcatttaccg caatttgaat tagtagccat agcagatccc cagatcgata ggaattgggc
     7081 agaaaaatta gggattgcaa tcgtgtctac tcaatcagaa gatgtgctat gccaccctga
     7141 cttggatgcc atattgatcg cttcaccatc tcatttacat cttgaacaaa ttattacagc
     7201 gagtattcat gagaaagcaa ttttttgtga aaaaccgatt ggtttgaatg aagaagagat
     7261 aaataaaaca ttaaacataa taaaaaacaa taatacttta ttgcaacttg gattcaatcg
     7321 tcgatttgac ccaagttttg ccaacattca gaaaagagta cagtttggtg aaattggcac
     7381 tccccatatt cttcgaatta cttctcgtga tccggcttgt ccatccaaag agtattgcgc
     7441 gacctctggt ggaatattta tggacatgtc tattcatgat tttgatatgg cacgtttttt
     7501 aatgcaatcc gaggttgtgg aggtttatgc aacaggggct gtcctaatta atcctgattt
     7561 tgagcagttt gatgatgtag atacagcaat cattcaacta cggttcgcta atggcgcatt
     7621 tggtgtcata gataacagcc gtcaagcaat ctatgggtat gatcaaagga tagaagtatt
     7681 tggttcagct ggtatgttat tggctgaaaa tcagttggag catacggtat ctcgatactc
     7741 ggctaatgga acggatggcg caaaacctca atattttttc cttgagcgtt atcatcaagc
     7801 ttatctggca gaactgaatg cgttttatag tgcctggaat gataatcaga taagtcccgt
     7861 ttcaggattg gatggactac aagccttgcg catcgcaaaa gcagcgaaac aatctttaga
     7921 aacgcatctt ccagtagtat tgagttgagg ccgttatgaa acagtttccc ggattttatt
     7981 ttgataagaa tagagcaatc gatttgattt gtatgggacg cgtagcagtt gatctctacg
     8041 cggagcaaat aggactggat ttgaaggatg ttgcaagctt taaaaaatat ttgggtggtt
     8101 gtgcaggaaa tattgccgtt ggagctgccc gattaggatt aaaaagccag atgttttcct
     8161 gtgttggctc agatgagtta ggtcagtttc tgaaagaaga attggaacgt gagggagtta
     8221 atattgagtt attatcggaa acagataatc atttaactgg ccttgtactt ttgggtatta
     8281 agcctccctc caatttccct ctgctttttt atcgtaatga ttgcgcagat atgcagatta
     8341 aatcagatca aattagtttt actgatttaa aaaatgcaaa ggcattattg ataacgggca
     8401 ctggtttatc cacagaatca atgaaaaata ctaccctgga agtagttcat ttagcccgaa
     8461 aagcagaaac gacaatagtt tttgatctcg attatagacc tgtcctttgg cggttaactg
     8521 tgattggtaa tggggaaagt cgttattgcc agaatgaata tgtaactcaa gtgtatcaac
     8581 aagttttgcc tttatgtgat ttgattgttg ggactgaaga ggaaatatgt attgcaggcg
     8641 gttcgaatga tattgaagtt tcattaatca atatcaggca gcgaaccgat gcgccaatag
     8701 ttgttaaatt aggagagaag ggtgctcgtg ttcattttgg agcagacaaa ggtctattac
     8761 aggcaaaatc ttttcctgtt gaagtattga atgttttggg agcaggtgat ggttttatgt
     8821 ccgggctttt agctggtttg ctgcaaggaa aatcatggga gcaagccgta acctatgcta
     8881 atgcttgtgg cgccttggtc gtgactcgtc atggttgcgc tcctgcaatt ccatacaagg
     8941 aagaattgaa ctattttatt caagaatatg atcatgatcc tgaaatatgg aacagctccc
     9001 agttaacaca attgcatgag aaattaagta aaaatcaaaa gacgcagttg cttattaaaa
     9061 atccatgtgg atttgccgat ggtttaaatt cgattgtaac gatgcaagac tcacctacag
     9121 caatgagttt tagttccctt aaattaaata aggggcaaag ccatcaattt agctcgagtt
     9181 atgagtttgc cgccctctta atgacaggaa aagttgtttt ccagtatggc tcttatagcc
     9241 aggaggttga acgtacagat tatttttctc aatcacccta tgtcttgcac tgctcaaaga
     9301 atgaagtaag tacagtaatc gctctgactg actgtgaaat tttattaatg gaaacggaaa
     9361 acgaaaattt attttcaccg attttatttg atgcatccaa tatgcttgag ctagacaata
     9421 ggggagaagg aattcttgaa aatacttcat atcgacttgt cagaacggta tttgataaac
     9481 gaaacagacc cgaatctaat ttggtggttg gggaaattat tacgtttcaa gggcgttggt
     9541 caagttatcc ttcacattat catcaacaac ctgaaattta ccattacagg ttttcagagc
     9601 cccaaggcta tgcttttggc gaaaatggta aagaggtgat gcgaattgaa cactatgata
     9661 cttaccagat tgccaataat caggaacatg ctcattgtgc agcgcccggt tatgcacttt
     9721 atacactgtg gtttatccgg catttaaata ataacgcata taccttacct accttcacaa
     9781 aagaccatga atggacaagg acaagcaggg caaatttaag agcatggcaa cagaaacagt
     9841 agtaatcctt atctgcagta tgattttgag agttaaataa atgaagatta aattaactat
     9901 ggctcaggcc ctattaagat ttttagctaa tcagtatgtc gttctggata ataatgaata
     9961 ccgtttcatc aatggcgtat ttggaatatt tggccatggt aatgtaaccg gattaggtga
    10021 ggcattggaa tacgatgctc agggtttaat ttattatcag ggacataatg agcaaggaat
    10081 ggctcacgca gcaaccgcct atgccaaaca aaaaaatcgg ctagggataa tggcttgtac
    10141 ttcatcaatt ggtcctggcg ctactaatat gattactgca gcggcgacag cgacaaccaa
    10201 tagaattcca ttactattat tgccaggtga tgtattcagt tgcaggcaac cagatccggt
    10261 tctccaacaa ttggaggtgc catatgacta caccatatct gttaatgatt gctttaaacc
    10321 agtaagcaag ttttgggaca ggataaccag acctgaacag ttaatgacag catgtatgca
    10381 tgctatgagg gtattaaccg atccagttga aacaggtact gttactttgt gtttgcctca
    10441 agatgttcag tctgaagctt atgaatatga agaatccttt tttaaaaagc gcttatggcg
    10501 tattgagaga gagaggataa gtgatgcaat gttggcagag gccatacggc atatcagaaa
    10561 ggctaagcaa ccattaatca ttgcaggtgg tggtgtgcat tattcactgg cttcggagac
    10621 tttatcagcg tttgcattaa aacatcgcat tcctgttgct gaaacccaag caggaaaaag
    10681 tagcctggca gcgaaacacc ccatgaacgt tggtggaata ggtgttactg gatctgaaat
    10741 agcgaattct ctggcagctc aggctgatgt tattttggtt atcgggtctc gattacagga
    10801 ttttacgaca gcttccaaat gggcttttaa aaaccaggac tgccaaatca ttcatcttaa
    10861 tgtatcacgt cttgatgcca tgaaaatgaa tggagttatg ttaaaaggag atgctaaaac
    10921 tggtttggag caattgggga aagagctggc agattatcaa acctctctct cttaccagca
    10981 gttaatacgt cattatcaaa atgaatgggc ccaagagtta gataaattaa ctcagtctta
    11041 ttctacagag caagctgggt tacatcaaac tactgttttg ggactgctca atcagtatgt
    11101 tactgaagag gatgtgataa ttagtgcagc ggggagtttg ccaggtgatt tgcatcgaat
    11161 ttggcaatcc aaaaaggcaa aagattatca tttagagtac gcttattcct gtatgggata
    11221 tgaggttgca gcgggtcttg gagtacgtat tgcaaaagaa aatcaggaag gagaagtcta
    11281 cgttttagtt ggagatggca gttttgtcat gatgcattca gagcttttaa ccgccattca
    11341 ggaacgcaag aaaattacaa ttctcatttt tgataatcat ggatttcagt gcattagaaa
    11401 tttacaggaa gggaatggta gtctgggttt tggtaatgaa tttcgttata gggagccaac
    11461 tactaatgct ttaaatggcg attatttacc catagatttt tgtaagtacg cagagggatt
    11521 gggggcagct acttattttg ttaaatccta tgagcaattc cggactactt tagactcagc
    11581 aaaaaaacag gaaaatagta cggttattgt tcttcctgta ttacccaaga ccatgagtca
    11641 aggatataag acttggtggc gggttggagt tgctgaggtg tctcaatcag aatcagtgag
    11701 taaagcttac gaaatgatgc aaaagcaaat tgaaacagtt agacaatatt gaagatggga
    11761 ttgatgatga gtattcaact tggtatcgcg ccaattaatt ggtgcaatga tgatgatccg
    11821 aatttaggta aaaacattag ttttgagcag tgtattaacg aaatggcttt ggcaggttat
    11881 tccggcactg agctggggaa taaataccca cgagatgcaa caacattaaa ggctgctttg
    11941 gaacaacgag gtttacaatt atccagttca tggtttagca cttattttac tgaggccaat
    12001 gcttttgaaa aaaccgtatc aagatttctt gaacacttaa gttttatgcg cgcattaggt
    12061 gcgtcgtata ttaatgtttg tgaatgtggt catgctattc aaggcactga tttacctata
    12121 ttaagtgctc atatccctca atttactcaa gagcaatgga gtttattgat tcaagggctg
    12181 cataccttag gccgaattgc ttacgacttc aatatgaaga tcgtttatca ttaccatgca
    12241 ggaactgggg tgttttggga gaaagacatt gattttttaa tgatcaatac cagtccaaaa
    12301 ttggtttctc tattacttga tacagggcat gctgtctttt caggaataga tccggttaat
    12361 ttaatagata agtacggtga tcgtattttg tatgttcatc tgaaagacat tcgccctgat
    12421 gttctgagac gagttgaaat ggagagactc agctttatgg atgcagtaag aagaggggta
    12481 tttactgtgc ctggtgatgg agcaattgat ttcataccta tatgggcagg gctgcaaaaa
    12541 catcgctata atggatggat gatagtggag gcggagcagg atccggaaaa agcgcatcct
    12601 ttggaatacg ccaaaaaagc atatgattat ttgcattcat ttattcatta aagaaaggct
    12661 gacgattgaa gatttaataa aatttacttt attaaaaatg ccagggaagc ttaagagaca
    12721 tctgtttata ggctgagatc tggttaagtt cactataata aggagattat ccatgtcaat
    12781 gaaggataaa ttgatacaat ctaccataag tgctttttta gttctcattg catcaaacac
    12841 agcaatagca gaggatttga gtaaggagaa atgttatggt gtggttaaaa aagggatgaa
    12901 tgattgttca acagcaactt cagattgcgc tgggtcttca accaaggata gccaaaaaga
    12961 tgcttttatt ttattaccca aagggatttg cgaaaaactt gttggtggat cattggatat
    13021 gggtaaataa aacattaatt taatttggga gttgaatatg aaacggattt acaggcattt
    13081 tattgctccg atattggcag tctttgcatt gtcgtcttat gtctacgcag aaccacaaac
    13141 tttgacctta gataatcagc atacttatgt gttatggaag gtcaagcatt taggcttctc
    13201 aactcaagct ggaaaatggt atgcaagcgg tcaacttgtt cttgataagg ataacccgca
    13261 gcagagtaaa gtgaatgtta ctatcaaggt cgatgacatc gtcacaggta tacccgagtt
    13321 ggataagcat ttgaaaggta aattattttt tgataccaag cagtttccaa cagcaacttt
    13381 tgttagtgac agagtggaag taacgggaaa gaataaagca aaagtgtatg gaatgcttac
    13441 tttgcatggc gtcacaaaac ccatcatatt gaacgtgata ttgaataaag caggaattaa
    13501 tttattaaat gacagagaaa cagctgggtt tagtgctacc acttcattaa agcgttctga
    13561 ttatggaata aaagctttaa taccagaagt aggagatgat gttgaaatag aaatagaagc
    13621 agaagcctat cttgataaga aatatggata atctatgcaa ttaaaaaata cttcaaccag
    13681 ctttggtctg gttgcgattt ttttccattg gattatggcc gctttaatta ttgggctatt
    13741 ggtagtgggt ttatacatgg tcaggatccc tattagtctt gagaaattaa aattatatgg
    13801 ttggcataag gagtacggat ttctggtatt gtttttggct tttttcagat tgatttggcg
    13861 tttgactaat caattaccgg aattggctat tcccctgtta gagaaaattg cagctcgcag
    13921 catgcactgg gctttttatt tttttatgtt tgctatgccc atcagtggat ggctaattac
    13981 ctcggctgca ggattgcctg cttctttttt tggtttattt gttttacccg accttgttgc
    14041 gcccgatgag aatagaagaa ttttgttcca attgattcat gaatggttgg ggtatgcatt
    14101 aattatggcc ataatcctgc atgcagcagc ggcattaaaa catcatttta ttaataaaga
    14161 tgatatatta cggagaatgt tatagtgaat gtctatatta aatcttccct ttgggtattg
    14221 atattttttg tgaatacatt gatttacgcc agtaaactcc ctgaatggga aatcgtgcca
    14281 gcacaaagtg agattacttt tactggtact caaaatggtg ccccggttac agggcgattt
    14341 aagaaattta ctggtcaaat ttttgctgac ccttcaaact acaagacagg gtcaattgat
    14401 atcactattg atatgaattc gattgcagca ccttttgacg acattgtgac aactttagcc
    14461 tccccggact ggtttaatgt caaagcattt cctaatgcag aatttaaagc ttcgaaattt
    14521 aatcaattgg atgataaaac ctatgaagcc gaaggtattc taactataag agataaatca
    14581 gctccagtta ctctgaagtt tgttgtggat caaatttcta acgatcaggc tttagttgaa
    14641 ggtagctcaa caatcaaacg cagtacattc ggggtaggtc aaggtgaatg ggagagtact
    14701 gatgaaatac aaaatgaagt gatcgtacgc tttaaaataa ctgcgacacg gaaaaaataa
    14761 ttgccagact tgcatgcaat ccataaatca gtagacctat gaaataagtt taattatttt
    14821 acaaggcagg cattatccga cacatttttg attaattact atactaatgg ttaatagtta
    14881 aatcaaaaat gtgtttggag ctaagaatgc ctataccttt actgaatttt aaacattttc
    14941 agaatgataa ttctattttt aggtcaacta cacaccataa ccatatggta tcacgaggaa
    15001 catttcctgg aaatcccaag acctggtttg ccaaagtgtg tagcgatcct gcatcagcaa
    15061 ggaacgaggt actcgcacaa gagtttttta gattaatcat ccctcaccag ccagaaacgc
    15121 ttattgcaaa agatgaggca caaaataatt actatgttta ttctgaagaa gtagaaggtt
    15181 tcaaaaacct accctatggt cagggtaaga gttttgagga cggaacattc actggtttag
    15241 ggcaagcggt ttttgtgtca gtatttttgc aagaagttga cttaaaaaat gggaatatag
    15301 gattagataa agacggtaga gtaattaaga tagatggtga tcagtgcatc gtatccatgt
    15361 taggcgtaga tcagagattt gatcttactc ctaaagtgat tgattcatta cctcggccgg
    15421 ttgattttgc aaccaataat tggttggatt ataaaagtag ctatttttct ttacaaagaa
    15481 tacttcaacc aactattaca aataatgaag tgttcagatc tgaagtcaat caggcgatgt
    15541 taaaaatttg ccttatacca gatgaatata ttgaacgttt tgtctccgta tacataccgg
    15601 atgggcataa gcccgataaa caaggttata ttgatttgat taaaagtcgt cgtgatgtct
    15661 taagattaag cgctgtaaaa aatgaatcat ttagaaatta cctatctgag ccagcagctc
    15721 aggatgatgc cagacatttc atggagcata tgaggagttt tcaatcaggt ggagaacctg
    15781 ttttggctag ccagcaagaa cgttctcagt taacaattga tttagcttta caaaatttga
    15841 agttagaaac agcttttatt caacagagtg agaaagtaac aacagtaaaa aatgctgtcg
    15901 agaaagaggc atttgctgat caggaaaatg aattggcaat ggtattccgt tttcaaatgg
    15961 aaatgaaaaa gcaagcgcaa ttatctgata gtagttttat cggtataatt tcccgctcat
    16021 ctcaggctag tgctgaaaaa acggcatacc aaaattttct caataaaact gtgaatccag
    16081 ttatggaaaa atgctttgct gccctggtga ggaatgatct taagcaattg gaacaggtat
    16141 tagcagagtt tcctaataac gaaaaatggt cagcatttaa cttacaagag tctggagtag
    16201 ctaaatcgca gatggatgcg gtaaagcgac ttattgaagg gagagtattt attcgaggta
    16261 aaatattgcc cgttttggat caatgtaaag atgctattga taaaaaagac atacccgctg
    16321 ctttgcaagc actcagtgca ctcccaccag acaaagatat gagcagaaca gcctcaatca
    16381 gtcctggttt aaaaggacaa atcatagaga tgaagcaaga tgtgactgag actctgaatg
    16441 caatagcaca acaaagcgcc attgcaacac ctgtaatcga tgatgctaaa agagtagagt
    16501 caggttatga tccactttta gttgaaataa ccaaacaaat cgaggcgctt gaaaatgagc
    16561 attgcgaaga tttgcccaaa ttagaaatgc atctcactac tgtgaatgat tgtttggaca
    16621 cagtcgaatt tctaataagc gaacacatta aacaacatgg tgcatcagaa gagcctcttg
    16681 agttgtctgg tttggaagaa ttgaggagcc gattacagaa aattaaaaaa acggatgaaa
    16741 acgctttaaa tacctatcca aaaaagaacg ctgaaataac acaaacagaa gccgcgctta
    16801 gtcaagtatt acctcaaaca agtaataata caaatcccgc aaaaagaacc attaagtctg
    16861 actacgatgt agatagcgaa aataaagtga caaaaattgg agaggacaga ccaggtttta
    16921 aaccagagta atcaaacagt atagtttcat atcaatatag ctttcaaaaa tctttccatg
    16981 ctatttaatc agggtcagaa cgcatataag acagcgattt tattgattat tatgaagtct
    17041 ttcattctag tgatatgagc tgttaaatag acag
//
LOCUS       DASLOE010000050        16937 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.050, whole genome shotgun sequence.
ACCESSION   DASLOE010000050 DASLOE010000000
VERSION     DASLOE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16937
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.050"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            151..1452
                     /locus_tag="U6309_001350"
     CDS             151..1452
                     /locus_tag="U6309_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN5504799.1"
                     /translation="MIQNNRICRWLFIVLLSLPLVGFTSIPVKSIVVFGDSLSDNGNT
                     THLLKSLRQEEDPAFLVAPFKIFVINKMIEFANDYYVPQMVLDAGISAVTDFFDHDLA
                     PYLANLVGKVKQVPVLPGKPYWKNRFSNGRVWIEYLAEMLSIQKSDEEVYLNKAFGGS
                     WSATYDYQLTVWNLIRHPLGTIKNLIVGKLIPPSLGLIVQAYLLEHEQLSDETLYFIY
                     SGSNDYINVLFFEDNYNTEVMSTYIDNVLDGLSSAVLKLANAGARRFVIMGIPHVGDT
                     PKFVKTTDRDVLNAAVDLHNERLAKRIDAWKERYPTADFLFVNTEQYLKKAFEDPEKY
                     GFYNVKDACIDVKFPMFNAFIHSPFARNYVLQYTQVLQYRDKQFGPGENNYHMCSEPE
                     DYLFWDEIHPTTRAHNYLAYEVCMALEEHGYEVTCKMPESA"
     gene            1811..3625
                     /gene="glmS"
                     /locus_tag="U6309_001351"
     CDS             1811..3625
                     /gene="glmS"
                     /locus_tag="U6309_001351"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEN5504800.1"
                     /translation="MCGIMGAVSERDISKILLEGLRRLEYRGYDSAGIAVIDSQDRLK
                     RVRIQGKVQNLADAMQETAIAGNTGIAHTRWATHGKPSEQNAHPHLSHGEIALVHNGI
                     IENHEHLRQQLITYGYQFTSETDTEVAAHLIHYHYQQHENLLLAVQKAAAEMQGAFAL
                     GVIHQKRPEELVAIRKGSPLVLGFGIGENFIASDALALRSFAQSVIYMEEGDSACVTT
                     QDIKVYDSNRILVQRAVHPLNSDSEIVNKGPYRHFMLKEIFEQSKVITDTLESRINSI
                     DVLRASFGEKASHIFPMVKNIHIVACGTSYHAGMIAKYWLESLAGLPTQVEIASEYRY
                     RDVVVPDNTLFITVSQSGETADTLAALFKAKQSNYLASLAICNVATSTLVREADCVFL
                     TRAGIEIGVASTKAFTTQLAAFLMLAAALCKDNRAQEVLRQLQELPACCERVLQMNEE
                     VESLASLFVNKVHALFLGRGVQYPVALEGALKLKEISYIHAEAYPAGELKHGPLALVD
                     KDMPVIAVAPNDELLDKLKSNLHEVSARGGQLFVFVDDSQNWKANGARLIKVPSCGAW
                     LAPIVYTIPLQLLAYHVAVAKGTDVDQPRNLAKSVTVE"
     gene            3622..4287
                     /locus_tag="U6309_001352"
     CDS             3622..4287
                     /locus_tag="U6309_001352"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HEN5504801.1"
                     /translation="MNKGQYFWNELWCEGRISFHKEEVNPDLIAYVSSLNIPAKGRIL
                     VPLCGKSVDMLWLVRQGYHVVGIELVEKAILQFVQEHQITVRENTIGQAKQYFTDNLN
                     LWVTDIFALNSALIEPVDAIYDRAALVALPKKLRPAYVDICLKWLKPGGSILLKTLQY
                     NQEKVQGPPYSVSPEEIALSYQQCAKIELLKSQKRIREPNDHLFNLGISEVNDYVWRI
                     RKG"
     gene            4411..6780
                     /locus_tag="U6309_001353"
     CDS             4411..6780
                     /locus_tag="U6309_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEN5504802.1"
                     /translation="MAEVMLASAAIIADELKVKVSQVETAIRLLDEGATVPFIARYRK
                     EATGGLDDVQLRFLAERLIYLRELDERRTVILQSIREQEKLTPELEQSILAAETKTRL
                     EDLYLPYRPKRRTKAQIAIEAGLEPLAHALWKDPALDPEEQASQFINSEAGIENTQAA
                     LDGARHILMEHFAEDAELINELREYLWQHGIVKSVGNSTKKDQANKFSDYFDYSEAIK
                     KIPSHRALALFRGRREGILQVSLVLPDQDANYGENKVAAYFNVTDRQRKADGWLIDTV
                     RMTWKIKLFTKLELELLTRLREIADEEAIKVFSKNLRDLLLAAPAGPQVTIGLDPGIR
                     TGVKVVVVDATGKLLDYSVIFPFAPQNEWHQSIADLAKLAAKYQVNLISIGNGTGSRE
                     TERLVADLIKMYPDLKLTKIIVSEAGASVYSASEIAANEFPDLDVSLRGAVSIARRLQ
                     DPLAELVKIEAKSIGVGQYQHDVNQAKLARCLDGVVEDCVNAVGVDINTASAALLSHI
                     SGLNDTLAKNIVQFRDEHGAFTNRNQLKNIHRMGEKAFQQAAGFLRIMNGDNALDASC
                     VHPEAYPLVEKIISDKKVSIKEIIGNRELLQSINAEQYVDENFGLPTIRDVLRELEKP
                     GRDPRPEFRTAQFQEGVEDIKDLEQGMVLEGVVSNVTNFGAFVDIGVHQDGLVHISAM
                     TNRFITDPRSVVKAGDIVKVKVVEVDKERRRIGLSMKLNDEQAPAVLQKANKPQPARK
                     QAEQKQPEFKKKQEHKKKPDKIKKSEPVKKTVFNTAMADALAKLKRGGE"
     gene            6783..7163
                     /locus_tag="U6309_001354"
     CDS             6783..7163
                     /locus_tag="U6309_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEN5504803.1"
                     /translation="MQTPKGELTIQTLAMPLNTNANGDIFGGWIVSQMDLAAGVLAKR
                     ISHGRVATVAINSMTFLKPVHVGDVVSCHVELVKVGNTSMTIRVEVWAYSSIKMEKYQ
                     VTEGIFVFVAIDENGKPRQVPKTQ"
     gene            7160..8800
                     /locus_tag="U6309_001355"
     CDS             7160..8800
                     /locus_tag="U6309_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2832 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504804.1"
                     /translation="MTNKTQELQRVIADMAVLIEQDAEPDPQLYAFFFQHPEISLELI
                     HLINNLSEELVHDGPPIYSACVFALDICIAQLQAAAEANNKNATKLLSQLMNHLAEVI
                     DTHKHSLSFWLPVLNAFYEVHVELTDDLKEAYYGLASQDDDAVEVDEQFNHLDSIKDL
                     IHELSDLTLFDIAESFFAQSYAMPPDFFADLIVDLYSIEEGKDIALLVLLHPNIEVRE
                     IALSTIDQLINTITLTPASLSRLQIIKYWYPTHYHGMFDRWIKTQRKKGVTFETEASQ
                     AKITIKATEVDGSGSQGVFIHARKNKKNRLCGLLLKYDLGIKDAWITPEISAAEVTDY
                     YHKAFEDSVTLREVDFSYFQTIVEHFLAITVEKSDIPNLHFLEILEFLGIRLKPDKLD
                     LDCVFEQLSVQIIPFTQEKIAESLKRSKMWLKNKQFTESWYIENPTIDKIVNHNSSFV
                     EGVKVCRLADAIDAVFSEEMELHRDKWQFHFLWIALWVKAKHKKNEKVWQDSFLIAYA
                     IHQGYPLKDIPVMKEICYQTVVNSIETMQERRTHLSQE"
     gene            complement(8855..10720)
                     /gene="vipD"
                     /locus_tag="U6309_001356"
     CDS             complement(8855..10720)
                     /gene="vipD"
                     /locus_tag="U6309_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector VipD"
                     /protein_id="HEN5504805.1"
                     /translation="MKLAEIMTKSRKLKRNLLEISKTEAGQYSVSAPEHKGLVLSGGG
                     AKGISYLGMIQALQERGKIKNLTHVSGASAGAMTASILAVGMDIKDIKKLIEGLDITK
                     LLDNSGVGFRARGDRFRNILDVIYMMQMKKHLESVQQPIPPEQQMNYGILKQKIALYE
                     DKLSRAGIVINNVDDIINLTKSVKDLEKLDKALNSIPTELKGAKGEQLENPRLTLGDL
                     GRLRELLPEENKHLIKNLSVVVTNQTKHELERYSEDTTPQQSIAQVVQWSGAHPVLFV
                     PGRNAKGEYIADGGILDNMPEIEGLDREEVLCVKAEAGTAFEDRVNKAKQSAMEAISW
                     FKARMDSLVEATIGGKWLHATSSVLNREKVYYNIDNMIYINTGEVTTTNTSPTPEQRA
                     RAVKNGYDQTMQLLDSHKQTFDHPLMAILYIGHDKLKDALIDEKSEKEIFEASAHAQA
                     ILHLQEQIVKEMNDGDYSSVQNYLDQIEDILTVDAKMDDIQKEKAFALCIKQVNFLSE
                     GKLETYLNKVEAEAKAAAEPSWATKILNLLWAPIEWVVSLFKGPAQDFKVEVQPEPVK
                     VSTSENQETVSNQKDINPAVEYRKIIAEVRREHTDPSPSLQEKERVGLSTTFGGH"
     gene            10972..11712
                     /locus_tag="U6309_001357"
     CDS             10972..11712
                     /locus_tag="U6309_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HEN5504806.1"
                     /translation="MGYRIEMATRNPFDIDHKSKYLREAALEANLSHPETTPTMLTCP
                     IDSGFLKDPVITPEGFVYNKSSILKWLETKKEDPQSRKPLTAKDLQPFPELLIIVNRF
                     VETQTNYEKLKNRLVQNARVAARQKEYTEIPDIFLCPISKTLIKTPVITAQGKVYDQE
                     ALSNFLIATGNKDETGKKLSIDDVVVFDELYQQIKVYNFYRKREMQKNQIQPSVSSGF
                     GFFSLNFLTSWLWGTEEKKEKTSSDMTY"
     gene            11833..>16937
                     /gene="sidH"
                     /locus_tag="U6309_001358"
     CDS             11833..>16937
                     /gene="sidH"
                     /locus_tag="U6309_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidH"
                     /protein_id="HEN5504807.1"
                     /translation="MKRTIETYIIYLKEDLKIADTCKTIKDGLLKSITDKTHFSEELA
                     TYFERDNPNAPFKVNTTDPTQVAVLKKLLNALENAEKSFRAIENIDISRDRYTAMIAK
                     DAVMVSYKAVHEIYAALQLINHSNSDIQDIVGPHIQKLLPQMALASKALGNFAPEHPE
                     ESAGAVLAGVVNMLPTEKPTESESLGKLSNLIFELPHYFEELQKLIATGASGIATKSI
                     TSAEDYQSAMIKKANETKYYFEQLSSKSGLLAIPSYLSIVKRLIAHSTDLVNAGAPLT
                     KQAYLDAVAKLEDIKHNILPQLISELEMVEESMGLKPGLLTDPALEQMNKYYTQLAEQ
                     VDNIAKAAGVLDTVSDYSDSIGGKIVRFLAGDSKKLDVGPKLTPAPDLGVLMDDVFIQ
                     KRRSNQESRLNEARLSSEDKSVLAAANRFFDKIGSYNSIHKAWSKWSLANISQSEKDA
                     LIKEYKQFQPHFAALYPDIDKLVVDALTQPTGSNIVSRLYSSDYKQLWSSDHFKQVLS
                     CKDSVLSSIQQSLAQSEFKAKLIEKTMSHSEETAYSMNNKTTNLTTRVQPFEPLKFTL
                     EDDKPVEYYHKRVIAASNQILELERAQKGVAEFFNYIQKKYPHENPSFDSLDESDKEF
                     LRKAYKTFQPQLLALKHDDINTRLVSSLTSSKPTDPPLRLTDLVSLKSGINDYLNEKI
                     SDLNQDKTTLLDKEEEAREEQYAKNPLVAKGAELEKQTLFGQMSKLKLSKSVDDFFNK
                     KFQTYLKDNLSPEVWKQLSSNGETLDFDKIPYLEFHKDSPEVAMYKQLINSMHYMKNG
                     LEKLESLNDYGDPNNIYHRTRFVMTTFNALVMNICFSKYYVMEAGNNPGLKAIVQEGL
                     DLLKPLEGMPLIGDYLKTTEKQEPPKQNIITAWKKQQAVVESGLSKGPKPKTDQQLIS
                     EQLGKIQEAIDNFDGDLEVSDSAREKIKTQIGEFAKGISGLSFGPGSVKKILAALTKL
                     ETQLSNLDKESPEVTLGKLKDIHSELNAQFRAAAEYTEYHSGQKFGSYSNNISTIVSN
                     FCNGLVSNLPLKQEPAPKVKAPEKPVTPVITGTTNPHEVVFGTKHEEFNSVYQPYLLL
                     KRITDEFRDQNNPYKPSFDELKEEAVSYYDKIQPLLESVDPKFDKNFIAKHKESSTLL
                     KAIDEVMSMRQRINNPESSFAKLKDLHLEGDFEKEENKEKFRQLYKEIQPYLHKIDST
                     YDQTQFLEGLKTAKDFSGALQRIMNEENALQQSTSLKDTSYLQLIAESLYQIPVKLNK
                     LKAEPDTPEPSKEEIDANVKAFVEGLNGLSFGPGSVKKILSTAAKLQMQLSDIGKEGR
                     ELTMGRLKEIQAEFGTILMAAADNAEFHLGLKPGTYSRTVSERFEKFYSSLIVNLPLE
                     KDQTGLELLIDTTSTQKRLAREMERLESVKEDTSAIDTKKSIFGTEHEQFSTLYQPYA
                     SLRHIAKDIEDGVHMNLYERTLEELKEEASNEYKKIQPYLAKINPEFTEDYISKTDGE
                     YSLLHAIDRVFEERHKINKPSSPFDKLRDLYLDGDFEKEENKEQFLQLYAELQPHLIK
                     INYQYDLAYFLRELQTPEDFKAATERIINDESKLQELITGLDDTKRLKVKLCEERIGY
                     FIDLLKKQELEVGPEKIQAFKEKIFFNYIHANVNSALDAKIGSHAEQFLQFIEKDFLD
                     KKNEILEKITIDQDMEEEIAKAIDRIAPDIINN"
BASE COUNT         5400 a         2944 c         3544 g         5049 t
ORIGIN      
        1 ttttaacggc atattaggtg aaaaatagtt gcgttattgc atgttagtac ttaagtataa
       61 agaatcgttg acaataatta aattctgatc atactctaag agttaagctg ctatcttttg
      121 tttcgctagc tttgatataa ggagattttt atgatccaaa acaacaggat ttgtcgctgg
      181 ctcttcattg tattgttgtc tctccccttg gttggtttta cgtctattcc agtgaaatca
      241 atagtggtgt ttggagatag cttgtctgat aacggtaata ctacccattt acttaaaagc
      301 ctgcgtcagg aagaggatcc tgcgttctta gtggctcctt tcaaaatttt tgttatcaac
      361 aaaatgatag aatttgctaa cgattattat gttccacaga tggtacttga tgcaggaata
      421 tcagcagtta cagatttttt tgatcatgat ttggctccct atcttgccaa tttggtgggt
      481 aaagtaaaac aggtcccggt gctgcctggc aaaccttatt ggaaaaatcg cttttctaat
      541 ggtcgtgtct ggattgagta tttggcagaa atgttatcga tacaaaaaag tgatgaagaa
      601 gtctacctaa ataaggcatt tggaggtagc tggtctgcca cttatgatta tcaattaaca
      661 gtttggaatt tgatacggca tccattggga acaatcaaga acctgatagt aggaaaatta
      721 attcctccga gtcttggtct gatagtgcaa gcttatctgc tggaacacga gcagttaagt
      781 gatgaaacgc tgtattttat ttattcagga agtaatgact atattaatgt cctgtttttt
      841 gaagacaatt acaatactga agtaatgagc acctatattg acaatgtgtt ggatggtttg
      901 agttctgcgg ttctgaaact ggctaatgcg ggagcgagac gttttgttat tatgggaata
      961 cctcatgtgg gggatactcc caagtttgtg aaaactaccg accgtgacgt gttaaatgca
     1021 gcggtagacc tgcataatga gcgccttgca aaacgcattg acgcatggaa agagcgctat
     1081 ccaacagctg acttcttgtt tgtgaataca gagcaatatc tgaaaaaagc gtttgaagat
     1141 ccggagaaat atggttttta taatgtgaaa gatgcctgta tcgatgtgaa gttccccatg
     1201 ttcaatgcat tcattcactc tccatttgct cgtaactacg tattgcaata cacacaggtt
     1261 cttcaatatc gagataagca atttggtcca ggtgaaaata actatcacat gtgtagtgaa
     1321 ccagaagact atttattctg ggatgaaatt catccaacca cgagagcaca taactatctt
     1381 gcttatgagg tttgtatggc tttggaggaa catggctacg aggtaacttg caaaatgccg
     1441 gagtctgcct aatacaatta ggcatcatca cctgaatcac atcaacagtg gttcaggtga
     1501 tttcatgatg aagttgtcaa aattgatcct catttaagta aatccagagt ggtttttaag
     1561 caatttttga atctctaaat gatgacgaag attaatttgc cttagtgatt tgtaaaactt
     1621 tatgtcataa ttcccctttt ttattgatat acttcattca tatgagggaa tattatttat
     1681 aaaatttaag gcaccggtta tttaggaaat gagctgcatt cttatgcgtt ttactcttta
     1741 tctagtagat gaaccaagaa tgaaaaaagc ttatttctat aaaaacagtt gctgaaaaga
     1801 ggagcaggtt atgtgcggga ttatgggggc tgtttcagaa cgggatatta gcaagatttt
     1861 attggaaggc ttgcgccgat tggaatatag agggtacgat tctgcaggta ttgccgtaat
     1921 agatagtcaa gatcgtttaa agagagtaag aatccagggg aaagtacaga atttggcgga
     1981 tgctatgcaa gagaccgcta tcgctggaaa tacaggtata gctcatactc gttgggcgac
     2041 gcatggtaaa ccctccgaac aaaatgctca tcctcattta tctcatggag aaatcgcgtt
     2101 agttcacaat ggcattattg aaaatcatga acatttgcgc caacaactta ttacttatgg
     2161 ctatcaattc acatctgaaa cagatacaga ggttgctgcg catttaattc attatcatta
     2221 ccagcagcat gagaatttat tactggctgt acaaaaagct gctgcagaaa tgcaaggggc
     2281 ttttgcattg ggagtgattc atcagaaaag accggaagaa ttggttgcaa ttcgtaaagg
     2341 gagtcctttg gttttgggat ttggaattgg tgagaatttc attgcgtctg atgccttggc
     2401 actcagatct ttcgcacaat cagttattta catggaagag ggtgacagtg cttgtgtcac
     2461 aacacaggat attaaggttt atgattccaa tcgaatactt gttcaaagag cggttcatcc
     2521 gttgaacagt gattctgaga tagtgaataa agggccatac aggcatttta tgctgaaaga
     2581 aatatttgaa caatcgaagg taatcacaga cacgttagaa agtcgaatta atagcattga
     2641 tgtgctaaga gcgagttttg gtgagaaagc atcccatatt ttcccaatgg taaaaaatat
     2701 tcacattgtg gcatgtggaa ccagttatca tgctggaatg attgccaaat attggcttga
     2761 atcacttgcc ggcttaccta cacaggttga aattgcaagt gaatatcgat acagagatgt
     2821 tgttgtcccc gacaatacct tatttatcac tgtatctcaa tccggagaaa cagctgatac
     2881 tcttgctgcc ctatttaagg caaagcaatc gaattatttg gctagtttag ctatatgcaa
     2941 tgttgcaacc agtactttag ttagagaggc ggattgtgtt tttttaactc gggcaggtat
     3001 tgagattggt gtagcatcta ccaaagcatt tactacacag ttggcagcct tcctgatgtt
     3061 ggcagcagcg ctttgtaagg acaatcgtgc gcaggaagtt ttaagacaat tacaagagct
     3121 gcctgcctgt tgtgagcgtg ttttgcagat gaatgaagaa gtcgaatcat tggcttcttt
     3181 gtttgtaaat aaagttcacg ccttattttt aggtcgaggg gttcaatatc ccgtagccct
     3241 ggaaggcgca ttaaaactta aggaaatatc ttatatacat gcggaagctt atcctgccgg
     3301 tgaattaaag catggtccat tggctttggt tgataaggat atgcctgtga ttgcagtagc
     3361 ccctaatgat gaacttttag ataaattaaa atccaattta catgaagtga gcgccagagg
     3421 tgggcagtta tttgtatttg ttgatgattc acaaaattgg aaagcgaatg gcgctcgtct
     3481 aatcaaagtt ccatcctgtg gtgcttggct tgcgcctatt gtttacacta ttcctttgca
     3541 attgctggcc tatcatgttg ctgtagctaa agggactgat gtggatcaac ctagaaacct
     3601 tgcaaaatcg gtgactgtgg agtgaacaaa ggtcaatatt tttggaatga gttatggtgt
     3661 gagggacgta tttcttttca taaagaagaa gtaaatccag atttgatagc ctatgtctct
     3721 tccttaaata tacctgccaa gggaaggatt ctggttcctt tatgtggaaa aagtgtcgat
     3781 atgctttggt tagttcgcca agggtatcat gtggttggta tcgaactggt tgaaaaggct
     3841 attcttcagt ttgttcagga acaccaaatc actgttcggg agaatacgat aggtcaagca
     3901 aaacaatatt ttacagataa cttgaattta tgggttaccg atatttttgc tttaaactca
     3961 gctttaatag aaccagttga tgctatctat gacagagcag ctttggttgc tttgccaaaa
     4021 aaattgcgac cagcgtatgt agatatttgc ctcaagtggc ttaagcctgg gggaagtatt
     4081 ctgttaaaga ctttacaata taatcaagag aaagtgcagg gtcctccgta tagtgtttcc
     4141 ccagaagaaa tcgctttatc gtatcagcaa tgtgccaaaa tagagttgtt gaaatcgcaa
     4201 aaacgaattc gggagcctaa tgatcattta tttaatcttg gtattagtga agtgaatgat
     4261 tatgtttggc gtattaggaa ggggtaaaac gtagtacagt gttacgcata aggacatttt
     4321 gtatggatat tagcttaatt tctatatcgc attatagtat gtgatgagta tataatgaga
     4381 cgatttttat catgttttac ggggatatga atggcagaag tgatgttggc gtcagccgca
     4441 attattgcag atgaacttaa ggtcaaagta tcgcaggtag aaactgctat tcggctgtta
     4501 gatgaaggag caacagtacc ttttattgcg cgttatcgca aagaagcaac gggagggctt
     4561 gacgatgtgc aattgcggtt tcttgcggaa aggctaattt atttgcgtga attggatgag
     4621 agacgaacag tcattttgca atccattcgt gaacaagaaa aattaacacc ggaattggaa
     4681 caaagcatat tagctgctga aactaaaaca cgcctggaag acttgtattt accctatagg
     4741 cctaaacgtc gtacaaaagc acaaattgct attgaagcag gattagagcc attagctcat
     4801 gctttatgga aagatcctgc tttggatccc gaagagcagg cttcccaatt tataaatagc
     4861 gaagcaggga ttgaaaacac tcaggccgcc ctggatggtg cgcgacatat tttaatggag
     4921 cattttgctg aagacgccga gttaattaac gaattacgag agtatttgtg gcaacacgga
     4981 atagtcaaat cagtaggaaa cagtactaaa aaggatcagg cgaataaatt ttctgattac
     5041 tttgattatt cggaagcgat taaaaaaatt ccatctcatc gggcactggc tttgttccgg
     5101 gggcggcgtg aaggaattct gcaagtaagt ctggttttac ctgatcagga tgccaattat
     5161 ggagagaata aggttgctgc ttactttaat gtcactgaca ggcaaagaaa agcggatggc
     5221 tggcttatag atacggtcag gatgacttgg aaaattaagt tatttaccaa gctggagctt
     5281 gagttattaa ctcgtctgcg cgaaatagca gatgaggaag ccattaaagt gttttccaaa
     5341 aatttacgtg atctgttgct ggctgctccc gcaggtcctc aagtgacgat aggtcttgac
     5401 ccgggaatta gaacgggtgt taaagtggtt gtcgtggatg ctacaggtaa gttgttggat
     5461 tactctgtta tttttccatt tgctcctcaa aatgaatggc atcaatccat tgcggatttg
     5521 gccaagcttg ctgcgaaata tcaggttaat ttaatcagca taggaaatgg caccggttct
     5581 cgggaaacag agagattggt agctgattta attaaaatgt accctgattt gaaattaaca
     5641 aaaataattg tcagtgaagc cggggcatct gtttattccg catctgaaat tgcagccaat
     5701 gaatttcctg atttggatgt ttccctaaga ggcgctgtat ctattgcacg aagattgcaa
     5761 gatcccttgg cagaactggt aaaaattgaa gccaaatcaa taggtgttgg ccagtatcaa
     5821 catgatgtga accaggcaaa gttggcgcgt tgcctggatg gtgttgtcga ggattgtgtg
     5881 aatgctgttg gcgtagacat taatacggcc tctgcagctt tgctatccca tatttccgga
     5941 ttgaatgata ctttagctaa aaatattgtg caatttcgcg atgaacatgg tgctttcaca
     6001 aacagaaatc aattaaaaaa tatccatcgt atgggagaaa aggcttttca gcaagcagcc
     6061 ggctttttgc gcattatgaa tggagataat gctttagatg cctcttgtgt tcatcctgaa
     6121 gcctatcctt tagtggaaaa aattatttca gataaaaaag ttagtattaa ggaaataatc
     6181 ggcaatagag aattattgca gagcattaac gctgaacaat atgtggatga gaattttggt
     6241 ttgccaacaa taagagatgt tttacgtgaa ttggagaaac cgggccgaga tccacgacca
     6301 gaattcagaa ccgctcaatt tcaggaaggt gttgaggaca ttaaagattt ggaacaagga
     6361 atggttctgg aaggtgttgt gagtaatgtg actaattttg gtgcctttgt tgatattggc
     6421 gttcaccagg atgggttggt acatatttca gcaatgacca atcgctttat tacagatcct
     6481 cgttctgttg ttaaagccgg tgatattgta aaagttaaag tggttgaagt ggataaagaa
     6541 cgccgaagga ttggtttaag tatgaagctg aatgatgagc aggctcctgc tgtcttgcag
     6601 aaagctaata aaccacagcc ggctagaaaa caagcggaac aaaagcagcc tgaatttaag
     6661 aagaagcaag aacataagaa aaaaccagat aagataaaaa aatcggagcc agtcaaaaaa
     6721 accgtattta atacagccat ggccgatgct ttggccaaac taaaacgtgg tggagaatag
     6781 ggatgcaaac acctaaagga gagttgacta tccaaacttt ggccatgcct ttaaatacta
     6841 atgccaatgg cgatatattt ggcggatgga tagtctccca aatggatttg gcagcaggtg
     6901 ttttggcaaa gagaatttcc catgggcgtg tcgctaccgt tgcaattaat tcaatgactt
     6961 ttttaaaacc tgtgcatgta ggcgatgtag tgagctgcca cgttgaatta gtgaaagtag
     7021 gtaatacctc tatgactatt cgggttgagg tatgggctta ctcatcgatc aaaatggaaa
     7081 aataccaggt tactgaaggg atatttgttt ttgttgcgat tgatgaaaat ggaaagccga
     7141 ggcaggtacc caaaacacaa tgactaataa aactcaagaa ttgcagcgag taattgctga
     7201 tatggctgta ttgattgagc aagatgcaga gcctgaccct caattgtatg cctttttttt
     7261 ccaacatccg gaaattagtc ttgaattaat acaccttatt aataatttga gtgaagaatt
     7321 agtgcatgat gggcctccaa tatactcagc atgcgttttc gctttagaca tttgtattgc
     7381 tcaattgcaa gcagctgcag aggcaaataa taaaaatgcc acaaaattat tgtctcaact
     7441 aatgaatcat ttagctgagg tcattgatac acataagcat agcttaagct tttggttacc
     7501 cgtactgaat gcattttatg aagtgcatgt tgagctaact gacgatttaa aggaagcata
     7561 ttatggcttg gcaagccagg atgatgatgc ggtagaagtg gatgagcaat ttaatcattt
     7621 ggactccata aaagatttga tccatgaatt atctgattta accctgtttg acatagcaga
     7681 gagttttttt gcacaaagct acgccatgcc gcctgatttt tttgcagatt tgattgttga
     7741 tttgtatagt attgaagaag gtaaagatat tgcactactg gttttactcc atcctaatat
     7801 cgaggtacga gagatagcgc tatctactat cgatcaatta ataaatacaa ttactttgac
     7861 gccagcatca ctgtcaagat tacaaataat caaatattgg tatccaaccc attaccatgg
     7921 aatgtttgat cgttggatta aaacgcaaag aaaaaaggga gtaacatttg aaacagaggc
     7981 tagtcaagca aaaataacga ttaaagcaac tgaagtggat ggcagtggtt cgcaaggagt
     8041 ttttatccac gcaaggaaaa ataaaaaaaa caggttatgc ggcttattat taaagtatga
     8101 tttgggcatt aaagatgcct ggataactcc agagatttct gcagcggaag ttacagatta
     8161 ttatcataaa gcgtttgaag acagcgtgac cttgcgtgaa gttgatttct cttattttca
     8221 aacaatagtg gagcattttt tagccattac tgttgaaaaa agtgatatac ctaatttgca
     8281 ttttttagaa atactcgaat ttctgggcat tcgtttgaaa cctgataagc ttgatttgga
     8341 ttgcgtattt gaacaattaa gtgttcagat tattcctttt actcaagaaa aaatagcaga
     8401 atctttaaaa cgctcgaaaa tgtggcttaa aaataagcaa tttacagaat catggtatat
     8461 tgaaaatccc accattgata aaattgtcaa tcataacagc agctttgtgg aaggagttaa
     8521 agtttgtcgt ttggctgatg ctatagacgc agtattttcc gaggaaatgg aattacaccg
     8581 ggataaatgg cagtttcatt ttttatggat agcgttatgg gttaaggcaa aacacaaaaa
     8641 aaatgaaaag gtttggcagg atagtttttt aatagcttat gctattcatc aaggataccc
     8701 attgaaagat attccagtaa tgaaagagat atgttatcaa acagttgtca acagcataga
     8761 aaccatgcag gaaagaagaa cacacttgag ccaggaatga atagaaaaat gttccggaat
     8821 tattcttgtt aataaactaa tatgtagaac agagttaatg gccgccaaat gtggttgaaa
     8881 gaccaaccct ttctttttct tgcagggatg gggatggatc ggtatgctcg cgccttactt
     8941 cagctatgat ttttctatac tctacggcag gattgatatc cttttgatta gagactgttt
     9001 cctgattttc agaggtagaa accttaaccg gttcaggttg aacttcaact ttaaagtctt
     9061 gggcagggcc tttgaacagg gaaacaaccc attcaattgg tgcccaaaga agattcaata
     9121 tctttgtggc ccagctaggc tcagcggctg ctttagcttc tgcctcaact ttattcaagt
     9181 aagtttccag ttttccttca cttaaaaaat taacttgttt aatgcagagg gcaaaggcct
     9241 tctctttctg gatgtcatcc attttggcat cgactgtcag aatgtcttca atttgatcta
     9301 aataattttg cacgctactg taatcaccat cattcatttc tttgacgatt tgttcttgaa
     9361 gatgcaaaat agcttgtgca tgcgctgatg cctcaaaaat ctctttctct gatttctcat
     9421 caatcaatgc atccttgagt ttatcatgtc cgatataaag tattgccatt aatggatgat
     9481 caaatgtttg cttgtgagaa tccagtaatt gcattgtctg atcgtagcca ttttttacag
     9541 cgcgcgctct ttgttcaggt gttggagagg tgtttgtggt tgtgacttcc cctgtattaa
     9601 tgtaaatcat attatcaatg ttataataga ctttttcacg atttaacacg gatgaagtgg
     9661 catgaagcca ttttccacct attgtagcct caaccaaaga atccatgcgt gccttaaacc
     9721 agcttatcgc ttccatagca ctttgcttgg ccttattaac gcgatcttca aatgcggtgc
     9781 ctgcctctgc tttgacacaa agcacctctt ctctgtcaag cccttcaatt tccggcatat
     9841 tgtccagaat tcctccatca gcgatgtatt cacccttggc atttctgcca ggtacgaaaa
     9901 gtaccggatg ggccccagac cactggacaa cttgtgctat agattgttga ggagttgtgt
     9961 cttcactgta gcgttccagc tcatgctttg tttgattagt tacaactaca gacaagtttt
    10021 taattagatg tttattttcc tcaggcagca actctcttaa gcggcccaag tcaccaagag
    10081 ttagacgagg attttcaagt tgttctcctt tggcgccttt caactcagta ggaatactat
    10141 taagtgcttt atcaagtttt tccaaatctt ttactgattt tgtcaagttg ataatatcgt
    10201 ctacattatt tatcacaatc cctgctcttg ataacttgtc ttcatagaga gctatttttt
    10261 gtttaagtat gccataattc atctgttgtt ctggaggaat gggttgctgc acgctttcta
    10321 aatgtttttt catttgcatc atgtaaatga catccagaat gttacgaaaa cgatctcctc
    10381 tcgcacgaaa acctactccg ctattatcta atagcttggt gatgtccaat ccctcaatca
    10441 gttttttgat gtctttgata tccatgccaa cggctaatat actcgccgtc attgctccgg
    10501 cagatgctcc gctaacatgg gtcagatttt ttatttttcc gcgctcttgt aaggcttgaa
    10561 tcatacccaa ataggatatg cctttcgctc cgccgccact tagcaccaac cctttgtgct
    10621 caggcgcact aacagaatat tgtcctgcct cagtctttga tatttccagt aaattccttt
    10681 ttaatttacg gctttttgtc ataatttcag caagtttcaa acagaattta atttcttata
    10741 gttttattat agtagttaat tttgactttg tgatttttaa acaaacaatt atgtgagcaa
    10801 ttgattgggg gaccttcctc gaaccactat aagcatgtag tcttttttac tggcagcttg
    10861 agatacggtg ctctttatta gcgaaagcac aaataatgaa ggagtgattc acctataaaa
    10921 caaataaatt gcataaaaat aaatctaatg gtaaaatatt gcatcattta gttggggtat
    10981 agaatagaaa tggcgacacg aaatcctttt gatattgatc ataaaagtaa atacttaaga
    11041 gaagcagcat tagaggccaa tttatctcat ccagaaacaa caccaacaat gctgacttgc
    11101 cctattgaca gcggatttct aaaagatccc gtgatcacac cagaaggttt tgtttataat
    11161 aaatcctcta ttttaaaatg gttagaaacg aaaaaagaag acccacaaag ccgtaaaccc
    11221 ttaacggcta aagatttgca accattcccc gagttattga ttatagtcaa tagatttgtt
    11281 gagacacaaa cgaactatga aaaattaaaa aacagattag tgcaaaatgc tcgggttgct
    11341 gcacgccaaa aagaatacac tgaaattccg gatatatttc tttgcccaat aagtaaaacg
    11401 cttatcaaaa cacctgtcat tactgcccaa gggaaagtat atgatcaaga agcattaagt
    11461 aactttctta tcgcaacggg taataaagat gaaacaggca aaaaattatc cattgatgat
    11521 gtagtggtgt ttgatgaact ctatcaacag ataaaagttt ataattttta ccgcaaacgc
    11581 gaaatgcaaa aaaatcaaat tcaaccttca gtaagtagtg gttttggctt ttttagcttg
    11641 aattttctca cctcatggtt atggggaact gaggagaaaa aagaaaagac atcatctgat
    11701 atgacgtact aatgccccgt ttttgcgcaa tattgattca aagtgaagta aattttgaga
    11761 gaaaaaacca ctatactggc atgaatcaac tatattaata atatagattg ttcatttgct
    11821 ggttgatgtg gcatgaaaag aaccattgaa acctacatca tctatttaaa agaagattta
    11881 aaaatagctg atacttgtaa aacaataaaa gatggtttgt taaaatcgat taccgataaa
    11941 acccattttt ctgaggaatt ggctacttat tttgaacgag ataaccccaa tgcccctttt
    12001 aaagttaaca cgactgatcc tacccaagtc gctgttctga aaaaattgct taatgctctg
    12061 gaaaatgctg aaaagtcgtt tcgagctatt gaaaatattg atatcagtcg tgataggtac
    12121 actgccatga tcgctaaaga tgcagtcatg gtcagttata aagcggtgca tgaaatttat
    12181 gcggctcttc aactaatcaa tcacagtaat tcggacatac aagatatagt aggcccacat
    12241 atccaaaaat tattaccaca aatggcttta gccagtaagg cattaggaaa ttttgctccg
    12301 gagcatcctg aggagagtgc cggggcagtg cttgctggtg tagtcaatat gcttccaact
    12361 gaaaagccaa ccgagtcaga aagtttgggt aaactcagta accttatttt tgaattgcct
    12421 cattacttcg aggagttaca aaaattaatc gctaccgggg catcagggat tgccaccaaa
    12481 tcaattacca gtgctgaaga ctatcaatcc gcaatgataa aaaaggcaaa tgaaacaaaa
    12541 tattattttg aacaactcag ttccaagagt gggctattag caatcccaag ctatctttca
    12601 atagtcaaaa gattaattgc ccatagtact gatttggtta atgccggtgc tcctctgaca
    12661 aaacaagctt atttggatgc tgtcgctaag cttgaagaca ttaaacataa tattttaccc
    12721 caattgattt cagagctgga aatggttgaa gaaagcatgg gtttaaaacc aggtttactg
    12781 accgatcccg ctcttgaaca aatgaataaa tactatacgc agctggcaga gcaagttgat
    12841 aatattgcca aggcagctgg agttcttgat actgtttcag attattctga ttcaataggg
    12901 ggaaaaatcg ttcgttttct tgctggagat agcaaaaaac tggatgtggg gccaaaatta
    12961 acgcctgcac ctgatttagg tgttttgatg gacgatgtat ttattcagaa gagaagatct
    13021 aatcaggaaa gccgacttaa tgaagccaga cttagctctg aagacaaaag tgtacttgcc
    13081 gcggccaatc gtttctttga taaaatcggc tcctacaata gcattcacaa agcatggtcc
    13141 aagtggagtt tggcaaacat tagtcagagt gaaaaggacg ctttaatcaa ggagtacaaa
    13201 cagtttcagc cacattttgc ggcattatac ccagatattg acaaactggt tgtagatgct
    13261 cttactcagc ctaccggaag caatatcgtt tccagactat acagttccga ttataagcaa
    13321 ttatggagtt ctgatcattt taagcaggta ttatcctgta aggactctgt gctatccagt
    13381 attcagcaaa gccttgctca atctgagttt aaagcaaagc tcattgaaaa aaccatgagt
    13441 cactcagaag agacagctta ttcaatgaat aataaaacaa ctaatctaac tactcgggtt
    13501 caaccgtttg aaccactaaa atttactttg gaggacgata aaccggttga gtattaccat
    13561 aagagggtta ttgcagcatc taaccaaatt ttagaacttg aaagagcaca gaaaggagta
    13621 gcggagtttt ttaattatat tcagaaaaag tatccccatg aaaacccatc gtttgattct
    13681 ctggatgaat cggataaaga atttcttcgc aaggcttata aaacatttca accacaatta
    13741 ttggcgttga agcatgatga tattaacact cgccttgtaa gcagcctaac cagttcaaaa
    13801 ccaacggacc ctcctcttcg cctcaccgat ctggtatctc ttaaatcagg tattaatgac
    13861 tatttaaatg agaaaattag tgatttaaat caggataaaa cgactttgct ggataaggag
    13921 gaagaagctc gcgaagaaca atatgcaaaa aatcccttgg tagcgaaggg ggcagaactt
    13981 gagaaacaaa cgctctttgg gcagatgagt aaattgaagt tatccaagtc tgttgatgat
    14041 tttttcaata aaaaatttca gacttatctt aaggacaatc tttctcctga ggtatggaaa
    14101 cagctctcat cgaatggaga aacgcttgat tttgacaaaa taccgtatct cgagtttcat
    14161 aaagacagcc ctgaagttgc catgtacaag caattaataa actccatgca ttatatgaag
    14221 aatggcttgg aaaagctgga gtcgttaaat gactatggtg accccaataa catttatcac
    14281 cgtacgcgat ttgtcatgac cacatttaat gctctggtta tgaatatctg tttcagtaag
    14341 tactatgtta tggaggcagg aaataatcca ggattaaagg caattgtcca agagggtttg
    14401 gatttgctaa aaccattaga gggtatgcca ctcataggtg attatttaaa aaccacagaa
    14461 aaacaagagc cccccaagca aaatattatt actgcatgga agaaacaaca agcggttgta
    14521 gaatctggtt tgtcaaaagg acctaagcct aaaacagatc agcaattaat tagtgagcaa
    14581 ttgggtaaaa ttcaggaagc catcgataat tttgatggtg acctggaagt ttcagattca
    14641 gccagggaaa aaataaaaac tcaaattggc gaatttgcca aggggatcag tggcttgtct
    14701 tttggccctg gttctgtgaa aaaaatactg gctgccttga ccaaactcga aacccagctt
    14761 tctaatctgg ataaggaaag tcctgaagta acacttggta aacttaagga cattcattcc
    14821 gaattgaatg cgcaattcag ggcggctgca gaatataccg agtatcattc aggtcaaaag
    14881 tttggctcct actccaataa cataagcact attgtcagta atttttgtaa tggtcttgtt
    14941 agtaatctgc ctttaaaaca agaaccggca ccaaaggtta aggcaccaga aaaacctgtt
    15001 actcctgtta ttactggtac aactaaccct catgaggtag tttttggcac aaaacatgaa
    15061 gaatttaata gcgtttatca gccctatctc ttgttaaagc gtattactga tgagtttcga
    15121 gatcaaaata atccatataa accttctttt gacgaattaa aagaggaagc ggttagttat
    15181 tatgacaaaa tccaacctct tctcgagtct gttgatccaa aatttgataa aaatttcata
    15241 gcaaaacata aagagtcatc aacactactg aaagctattg atgaagtgat gtcaatgcgc
    15301 caacgcatta ataatcctga aagtagtttt gctaaactta aggatttgca tttggaagga
    15361 gactttgaaa aagaggaaaa caaggaaaaa tttcgacagc tatacaagga aattcagccg
    15421 tacctccata aaatcgatag tacttatgat caaacgcaat ttttagaagg tttaaagaca
    15481 gcaaaggatt tcagtggtgc tttgcaaaga attatgaatg aggaaaatgc tcttcaacaa
    15541 agtacgtcac tgaaagatac ttcttattta caattaattg cagaaagcct ctatcaaatt
    15601 ccagtcaagc tcaataagtt aaaagcggaa cctgacactc cagaaccttc caaagaggaa
    15661 atagatgcga atgtgaaagc atttgttgaa ggattaaatg gattatcctt tggtccaggt
    15721 tcagttaaaa agattctgag tactgccgct aaactgcaga tgcagctttc tgatattggt
    15781 aaagaaggac gagaattaac aatggggcgt ttaaaagaaa ttcaagcaga gttcggcacc
    15841 attttaatgg cggctgctga taatgcagaa tttcatttgg gcctaaagcc cggtacgtat
    15901 tctcgtactg tgagtgagcg atttgaaaag ttctatagtt ctctgattgt caatttacct
    15961 ttggaaaaag atcaaacggg tttggagtta ttaattgata caacaagcac acaaaaacgt
    16021 ttggcaagag aaatggaacg ccttgaaagt gtcaaggaag ataccagtgc aatcgatacg
    16081 aaaaaaagta tatttggtac agagcatgag caattcagta ctttatatca accatatgcc
    16141 agcttaaggc atattgcgaa ggatatagaa gatggggtgc acatgaatct gtacgagagg
    16201 acattagagg aattaaaaga agaagcttcg aatgaatata aaaaaattca accttatctt
    16261 gctaaaatta atcctgaatt tactgaggat tacatttcca aaacagatgg tgagtactca
    16321 ttactccatg ccattgacag ggtattcgaa gagcgtcata aaattaataa accatccagc
    16381 ccttttgata aattaaggga tttatatttg gatggcgatt tcgaaaaaga agagaataaa
    16441 gaacaatttt tgcagcttta tgcggagctt caaccccatc ttatcaaaat aaactaccaa
    16501 tatgatctcg cttattttct tagggagttg caaacaccgg aagattttaa agccgcgaca
    16561 gaaagaatta ttaatgatga aagtaaactt caagaattga ttaccggttt ggatgataca
    16621 aaacgattaa aggtaaaatt atgtgaagag cgtataggtt attttattga tctgctaaag
    16681 aaacaggagc tggaagttgg gccagaaaaa atacaagcct ttaaagaaaa aatatttttt
    16741 aattacatcc atgctaatgt caatagtgcc cttgatgcta aaattggttc tcatgccgaa
    16801 cagtttctac aattcattga aaaggatttt ctcgataaaa aaaatgagat tttagagaaa
    16861 attactattg accaagatat ggaagaggaa attgcaaaag caatagacag aatagcccct
    16921 gacattatca ataataa
//
LOCUS       DASLOE010000051        16801 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.051, whole genome shotgun sequence.
ACCESSION   DASLOE010000051 DASLOE010000000
VERSION     DASLOE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16801
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.051"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            72..902
                     /gene="minD"
                     /locus_tag="U6309_001359"
     CDS             72..902
                     /gene="minD"
                     /locus_tag="U6309_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEN5504808.1"
                     /translation="MAKIIVITSGKGGVGKTTSSAAISSGLALLGHKTVVIDFDIGLR
                     NLDIIMGCERRVVYDFINVINGEANLNQALIKDKRVPNLCILPASQTRDKDALTLEGV
                     EKTLNELAKDFDFIICDSPAGIETGALMAMYFADHAIVVTNPEVSSVRDSDRILGILA
                     SKTKRAIENKAPVQEHLLLTRYDPERVERGEMLSVTDVKEILAIPLIGVIPESKSVLK
                     ASNTGTPVILDESSDAGIAYQDAIARFLGEERPMRFISIDKKGLFRRLFSKNKEEVTI
                     "
     gene            899..1168
                     /gene="minE"
                     /locus_tag="U6309_001360"
     CDS             899..1168
                     /gene="minE"
                     /locus_tag="U6309_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEN5504809.1"
                     /translation="MSIFNYLRRRASTASVAKERLQIIISHERSQRNTPDYLPKLQEE
                     ILAVIAKYVNISRDQVSVNLERMEDSAVLELNITMPDKALEDSNS"
     gene            complement(1484..3970)
                     /locus_tag="U6309_001361"
     CDS             complement(1484..3970)
                     /locus_tag="U6309_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEN5504810.1"
                     /translation="MPELILILAAIGVAGTLLTRHASISVWAISYTIFLGLVLQFGSP
                     GLLSQIVLWAIEAVLVLGSIKPLRREVLSKHLFQIIKKAMPSMSITEREALEAGTVSW
                     EGDLFSGNPNFSLLRNAPTVALTEEEQAFIDGPVSALCEMLDDWDITHNRTDLPPEVW
                     QFIKENRFLGMIIPKQYGGLGFSATAQMSILVKIYGRSITAATTISVPNSLGPGELLL
                     KYGTEEQKNYYLPRLADGREIPCFALTGPNAGSDAASIPDKGVVCRQEFNGELILGVR
                     LNWDKRYITLCPVATVIGLAFRMFDPDNLLGKGEDIGITCALIPATTSGITKGRRHFP
                     LNTGFMNGPTQGKDVFIPLDYLIGGASMAGQGWRMLMECLSAGRAISLPSSASGGAQA
                     VALASGAYARIRKQFNQPIGKFEGIEEPLARIASAAYIIDSSLTMAAAAIDNGAKPSV
                     AGAILKYHTTERARQLALDGMDIHGGKGICLGPNNYIGRGYQGSPIGITVEGANILTR
                     SLIIFGQGAIRCHPYVFKELESIRNNDLVAFDDSFFAHVGFVITNFTKSLIFGWTDAR
                     FSKSPASSVKRYYQLIHKYSSNLAFLADFSMIVLGGELKRKEKLSARLGDMLSCLYMA
                     SAVLKRFYEDGEPRADLPVVHWSCQQLLHECETAMHGVIINFPARWARIVLSLILKPL
                     GNKRHKPDDKLGHKVARILIEPNETRNRITRLVYKKPGDNCPLGRMEEAFHKICAVEE
                     LERKVMKAVKDNILHSLTFQEQILEALEHQILTPVEAKQLEEAEKARQDVIRVDDFAD
                     EELRRPSSLGEKVKSEKKIPARDDMETETA"
     gene            complement(4063..4848)
                     /locus_tag="U6309_001362"
     CDS             complement(4063..4848)
                     /locus_tag="U6309_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN5504811.1"
                     /translation="MRELIHFAHGNGFPSLCYKQLLEQLETRFDCCFIDKIGHDPDYP
                     VGENWHNLVAEIIASIKRQAEQPVIAVGHSLGGVLSLLAAIEQPQLFKAVIMLDSPLI
                     GAFKSSMVRLAKALGIIDRVTPAFRTKKRREHWQNYEQLISYLKTRDLFKTFTEECLN
                     DYIKYGLEYKEDGYYLRFDRHIEYQIYRTIPHVIPRYKGKLIVPAALIYGDKSTVVDR
                     IDIRYMKNHFNVKCFKTMGTHLFPMEHPKAVGKQVIEVIDAII"
     gene            complement(4845..5762)
                     /locus_tag="U6309_001363"
     CDS             complement(4845..5762)
                     /locus_tag="U6309_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HEN5504812.1"
                     /translation="MYLMREQENVWESGVAKEKNKLKPIDAPIYSYWQALYMSFYSKR
                     LFVDVGKHWRGIGLIYLLLVIAICSIPFSIRIASDFNKTFNQQIIEPLLQLPTVYVQN
                     GNVIFDKPMPYLIRNDKNQVVIIIDTTGKVADFTEEYPYLNILIKKNSINFLLPTPEL
                     LGSSPVNEQQGVPIVQPLGKDMNVVFDGKKIIQDNAINGLKYASQLMIYPIVVAILYS
                     IMVTVLLVIAFLGQVFSKIFFSFTISFKKSSRLLMVASTPMLVVLVIMLSLHFIFPGF
                     GVILLILLAAYYSYGVYGLRDESRKMVLK"
     gene            complement(5713..6804)
                     /gene="ugpC"
                     /locus_tag="U6309_001364"
     CDS             complement(5713..6804)
                     /gene="ugpC"
                     /locus_tag="U6309_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEN5504813.1"
                     /translation="MATLNLIEVSKQVGETTILNRISIEIKKGEFMVVVGPSGCGKST
                     LLRLIAGLDVLSSGKILINNQCVNDTEAAKRDMAMVFQNYALYPHMTVFDNMAYGLKL
                     RRFSKADINKRVTAVANLLHLTPYLDRKPQALSGGQKQRVAMGRAIVRSPAVYLFDEP
                     LSNLDAKLRTEMRHEIKRLHQQFNTTSLYVTHDQTEAMTMADRILVLNQGEVEQIGTP
                     QELYQSPATQFVAGFTGHYPINFISGKYDKKTDSIYTDIGITYSAPNWLHSVDDKAEL
                     IVAIRPEHIQLCSSQSQDAVELYVEFVDDMGADKLIQAKCVKNNEQINLRISAEQMVS
                     NRQLHVELPKLKLHVFDERTGKRVGEWRG"
     gene            complement(6804..7631)
                     /gene="ugpE"
                     /locus_tag="U6309_001365"
     CDS             complement(6804..7631)
                     /gene="ugpE"
                     /locus_tag="U6309_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HEN5504814.1"
                     /translation="MRQFKRFGSHGLILFFIGLMMAPLYLAVVAASHEGTVMMHSQLP
                     LIPGTSFFKNIKSVLTEGLAVTGGEPITSMLVNSLFMAFIIAVGKIILALGSAFALVY
                     FDFPFKKSCFALIFATMMLPVEVRIVPTFQVVASFGLLNSFSGLTLPLLASATGTFLF
                     RQFFKTISKELVDAAKLDGAGPLRFFIDIVLPLSRVQISALFVILFVYGWNQYLWPLV
                     ITTETKMTTIVMGIRYLAGVADQVPEWHYIMAVALIALVPPCMVVIVMQRWFEKGLK"
     gene            complement(7628..8506)
                     /locus_tag="U6309_001366"
     CDS             complement(7628..8506)
                     /locus_tag="U6309_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN5504815.1"
                     /translation="MSKFNHQRLFAWLFIVPQLIVTIIFFIWPACSALLQSFYYTDAF
                     GLHKNFAGLTNFFDLFLDPGYAKAIWVTFVIAISVTLITMSLGLLMATLINYRGKSQG
                     VYKTLLIWPYAIAPAVAAILWRFLCHPTLGWLTQVLEYLGIHFNYVNDANQALLVVIL
                     TASWQQFSYNFLFYFAALKAVPQTLVDAAIMDGANAWQRFWQIIFPILSPTTFFLLIM
                     NLIYGFFDTFGIIQVMTHGGPGNSTTNLIYKVYEDGFEGMDLGSSSAQSVLLMIIVIA
                     VSLLQFKYLEKKVHYE"
     gene            8626..9633
                     /locus_tag="U6309_001367"
     CDS             8626..9633
                     /locus_tag="U6309_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEN5504816.1"
                     /translation="MHSYLKKDLILHYIQINNPGPHSELTLAEGPNPGFNESQILVRV
                     KATALNRADLMQKYGKYPPPPGESDIPGLEVAGEVIVTGSKVKQFKPGDKIYGLVGSG
                     GYAEICPVEASLAHHIPSHWNYSLAAALPEALTTVYATLYDLGALSAGQTLLIHGAGS
                     GIASLAIQMAKLTAAKVITTVGDSSKIEKALALGADQIIHYKEQDFELLIEDNSVDLI
                     IDFVGGDYFNKHLHLLKPQGKLIQIACLKGSKVECSLALLMQKRLHIIGFVLRSQSIK
                     EKARLWKLAHDHWFNAFENDKIKPVIDSEFDLSQIETALQRMQQGSHFGKIVIRVGKQ
                     C"
     gene            complement(9648..9794)
                     /locus_tag="U6309_001368"
     CDS             complement(9648..9794)
                     /locus_tag="U6309_001368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504817.1"
                     /translation="MSQYVLIVQLWGIRIKVRRALDLSTKNTNSLDIAKAALEESVRI
                     ILSS"
     gene            complement(9801..11084)
                     /gene="clcA"
                     /locus_tag="U6309_001369"
     CDS             complement(9801..11084)
                     /gene="clcA"
                     /locus_tag="U6309_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HEN5504818.1"
                     /translation="MGKKILILYFVSVLLGILTGAIASLFQLTIQQMDHLLELLFHMT
                     ESYGLPVGIVSAMTSMVMLFIAWMMVKYIAPEAAGSGVQEIEGTLLHVRPIFWKRLIP
                     VKFFGGVLSISAKMVMGREGPTIQMGGNLGQMLGELFRLTRRRRDTLIAAGSAAGLAA
                     AFNAPLAGVLFVMEEMRNQFNYSFTNFKMVVICCVMATIVLHLIIGSQPAIQMEVFEL
                     PTLGSLWLFFVFGILMGLAGLLFNVTLMRTVGWLDKQNPKNKKYYILAVGFLVGYLAY
                     SYPATVGGGYVIISEALTMSPAFSVLCSLIVIRFIMTMLSYSSGVPGGIFAPMLALGT
                     LFGLGFFHLFQWLSMDFSIEPGMFAVAGMGALFAAAVRSPITGVVLVVEMTKNYSLIL
                     PLMVSCITATIVVQLARNEPIYTQLLERTLRLNAK"
     gene            complement(11077..13224)
                     /locus_tag="U6309_001370"
     CDS             complement(11077..13224)
                     /locus_tag="U6309_001370"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEN5504819.1"
                     /translation="MLQQYKTLGGVHVEFSQQELDYQQGIDFLLESLDSQRGAVFASS
                     FEYPGRYTCWDMGFYNPPLVMICQNNTICFEALNQRGEILLSFLYPLLKTTDLWEVIS
                     QTTSFCQLKIKSSDKVFSEEERSHQPSVFSAIRLLLNFFKTEEDSYLGLYGAFGFDLI
                     YQFEQLERCKPRNETQRDMVLYLPDEIYIVNHRKEEAFVRRYDFQYQGRSTQSLSREG
                     KYSPYQPIHKPEKNCDHEPGEYAEVVKKAKEKFSCGDLFEVVPSQTFYTHFAEKPSIL
                     FRQMREINPSPYGFFVNLGDEEYLVGASPEMYVRVQGKRVETCPISGTIKRGADAIED
                     AHNIQTLLDSEKEESELTMCTDVDRNDKSRICEAGSVKVIGRRQIEMYSRLIHTVDHV
                     EGILRDGFDAVDAFLTHMWVVTVTGAPKIWAMNFIEQHEKSPRKWYAGAVGWFGFDGN
                     LNTGLVLRTVRIEKGTAEIRVGATLLYDSIPEAEEEETRLKASAFLDILQKPWQKAKK
                     KTEDVPLVGKNKKVLLIDHQDSFVHTLANYLRQTGAAVTTVRYDKAIGYLQNNHFDLI
                     VLSPGPGKPVDFKLSETIDVILSKNIPIFGVCLGLQGIVEYFGGVLDVLEYPMHGKSS
                     VIRVLDNKGLFSGLGDCFKAGRYHSLYARAEALPNELLVTAVSEDDVIMAVSHEYLPV
                     HAVQFHPETILSLSDQVGLRIIINLMNMVKDNG"
     gene            13498..13794
                     /gene="gatC"
                     /locus_tag="U6309_001371"
     CDS             13498..13794
                     /gene="gatC"
                     /locus_tag="U6309_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEN5504820.1"
                     /translation="MTLSAKDLENISKLAYLNEDSEHSAKLTQEVSAIMDFVDQLKTI
                     DTSQIAPLFHPLALNQRLRPDEVTEAECLAELEAMAPLFEDNLYLVPKVISSDK"
     gene            13797..15248
                     /gene="gatA"
                     /locus_tag="U6309_001372"
     CDS             13797..15248
                     /gene="gatA"
                     /locus_tag="U6309_001372"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEN5504821.1"
                     /translation="MEHYSLAQLSKALHNREFSSVELTQHCINKIQSNKDLNAFISLD
                     EDQALKEAQSADLVLKNGEGKPLTGIPMALKDLFCTKRLNTTCASKMLANFQAPYDAT
                     IVTKFKQNGAIIIGKTNMDEFAMGSSNENSYFGSVKNPWDRERVPGGSSGGSAAAVAG
                     NLVPFAIGSDTGGSIRQPAAFCGISGIKPTYGLVSRYGMVAFASSLDQAGPFARSAED
                     LAMILHCMAGFDNKDSTSVDRVIPDYSAEIKKPVDKIRIGLPSCFFQPQVEKGIQDAI
                     HDAVKLFENLGAEIIEIDLKLQPLWVPCYYVIACAEASSNLSRYDGIRFGHRSKSAST
                     LIELITNSRSEGFGNEVKRRILTGTHVLSTGFFDAYYLHAQKVRRLIRDELITTLNSV
                     DVIIGPTTPTTAFKLGEKINDPIQNYLADVFTVAANLAGLPAISIPTGFENKLPIGLQ
                     LMGKHFSESRLLAIAHHYQQHTNWHLANPNKQG"
     gene            15252..16685
                     /gene="gatB"
                     /locus_tag="U6309_001373"
     CDS             15252..16685
                     /gene="gatB"
                     /locus_tag="U6309_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEN5504822.1"
                     /translation="MEWDTVIGLEVHAQLKTKSKLFSGASTAFGATPNSQTSFIDAGL
                     PGVLPVLNEQAIIMAIQFGLAIHGTINDLSVFERKNYFYPDLPKGYQISQYQKPIVTN
                     GYLNIQLGNNLEKTVHIARAHLEEDAGKSLHDAHTDYTGIDLNRAGTPLLEIVTTPCL
                     HSAEEAINYLKTLHQLVRFLGICDGNMQEGSFRCDVNLSIKPKGSSVLGTRTELKNLN
                     SFRFIEKAIAFEQARHQDILESGLSVIQETRLYNPDNNTTQAMRGKENENDYRYFPDP
                     DLLPIHIDKEQIEAIKNNLPDLPEAISKELKSTPSLNDEDINFILSSPDTYQYYKKIK
                     SLCPAADKTIINWLKGQYAAFLNEHNLTFETPPISAKTMAAFLSKIHEKKISSSIAKN
                     IFSMLCVGEEDIDAIIEREGYQQQNDNPALEEIVEQIIKQYPDQVTEYKAGKEKLLAF
                     FIGQAMKQTKGKANPEQINLLLKKHLG"
BASE COUNT         5489 a         3518 c         2892 g         4902 t
ORIGIN      
        1 aatgaatgaa taacgtgtaa actgttggtt aaatattgtg ttgaatcgaa aaaaatagga
       61 agggttcagc gttggctaaa ataatagtaa ttacatctgg gaaaggcgga gtaggtaaaa
      121 ccacttcatc agcagcaatt tcttcgggac ttgcgctctt agggcataaa actgttgtta
      181 ttgattttga tatcggttta agaaaccttg atatcattat gggctgtgaa agacgtgtag
      241 tttatgactt catcaacgtc atcaatggtg aagccaactt aaaccaagct ctcatcaaag
      301 acaaacgggt ccctaatctt tgcatactgc ccgcatcgca aactcgtgat aaagacgcac
      361 taactctgga gggagtagaa aaaactctca acgagctagc aaaagatttt gattttatta
      421 tttgcgattc ccctgctgga attgaaactg gagcacttat ggcaatgtat tttgctgatc
      481 atgccattgt tgttactaat cctgaagtct cttctgttag agattcagat agaattctgg
      541 gaatactggc cagcaaaaca aaaagagcta tagaaaataa agcacctgtt caagaacact
      601 tgttattgac acgatatgat cctgagcgag tagaacgagg tgaaatgttg tctgtaactg
      661 atgtaaagga aatattagct attcctttaa tcggtgttat acctgagtcc aaatcggttc
      721 ttaaagcatc caatactggt acgccagtta ttctggatga atccagcgat gcgggcattg
      781 cttatcaaga cgccatagca cgctttttgg gcgaagaaag accaatgcga tttataagta
      841 ttgataaaaa aggactattt cgtcgcttat tcagtaaaaa caaggaggaa gtgacaatat
      901 gagtatcttc aattacttac gcagaagagc ctctactgct tctgttgcta aagagcgctt
      961 acaaatcatc atttctcatg agcgttctca acgcaataca ccagactatt tgcctaaact
     1021 gcaagaagaa attcttgcag ttatagcaaa atacgtgaat ataagtcgag atcaagtgag
     1081 tgttaatctg gagcgcatgg aagatagtgc cgttcttgag ctaaacatta caatgccaga
     1141 taaagcattg gaagacagca actcataatt ctattaagtt ttttatactt ataggtatta
     1201 ttacactatc aaataatgta gtttacctac ctacttaaca tacgcattaa aacattatct
     1261 cctccaagaa tatttcaaaa acctttaaat aaaataccta aatgagttaa gcattacata
     1321 gatgggagtt aaagtcattc ttaatggctc tgtctgtaaa gaacaattga ttaaaaccaa
     1381 aattgataaa aacatatggg aatgattagc ctgactctaa ttactgaata gggttacatc
     1441 atgtaactct attcagcatt atagaaaagt atctagtata aaattaagct gtttcagttt
     1501 ccatgtcatc cctggctgga atcttttttt ctgactttac cttctcacca agtgacgagg
     1561 gacgacgtag ttcttcatca gcaaaatcgt ctacccttat aacatcctgt ctggccttct
     1621 cagcctcctc tagttgcttc gcttcaactg gagtcaatat ttgatgctca agcgcttcca
     1681 atatttgctc ctggaaagtc aatgaatgaa ggatattgtc tttaacagcc ttcatcactt
     1741 tgcgctctag ctcttcaact gcacaaatct tatgaaatgc ttcctccatc cttcctaagg
     1801 gacaattatc ccctggcttc ttataaacca atcttgtgat tcgatttctt gtttcattag
     1861 gctctatcag tattcttgct accttatgac ccaatttatc atccggttta tgccttttgt
     1921 tgccaagagg ttttagaatt aacgataaaa caattcttgc ccagcgtgct ggaaaattaa
     1981 tgattacccc atgcatagct gtttcgcact catgcaataa ctgctgacaa ctccaatgaa
     2041 caacaggtaa atcagcacga ggttccccgt cttcgtaaaa tcgttttaaa acagcggatg
     2101 ccatgtacaa acaactcagc atgtcaccta agcgtgccga taatttttct tttcgcttta
     2161 actcaccacc taaaacaatc attgaaaaat ctgctaaaaa agccagatta gaactgtact
     2221 tatgaattaa ttgataatac ctttttacag aactggctgg agacttgcta aaacgagcat
     2281 cggtccatcc aaaaataagt gactttgtaa aattagtaat aacaaaaccc acatgggcaa
     2341 aaaaggaatc atcaaacgca accaaatcat tatttctaat actttctaac tccttgaaaa
     2401 cataaggatg acaacgtatc gctccttgcc cgaatataat taagcttctc gttaaaatat
     2461 tggccccttc caccgtaatg ccaataggag aaccttgata tcctcttcca atataattat
     2521 ttggaccaag gcatatccct ttaccaccat gtatatccat tccatccagt gcaagttgtc
     2581 tcgctctttc agtagtatga tactttaaaa tagcccctgc gacagaaggt tttgctccat
     2641 tatctatagc cgctgctgcc atagttaaag aagaatcaat gatataagca gctgaggcta
     2701 ttctggcaag aggttcttct atcccctcga acttgccgat tggttgattg aattgtttac
     2761 ggattctggc ataggcgcca gatgctaatg caacggcttg tgcacctcca ctggcgcttg
     2821 atggtaaaga gattgcacga cccgcgctta aacactccat taacatccgc cacccttgac
     2881 cagccataga agcaccaccg atcaaataat caagcggaat aaaaacatct ttgccttgcg
     2941 ttggaccatt cataaaccct gtatttaaag ggaagtgcct gcgccctttt gtgatccccg
     3001 aagtagttgc aggaataagc gcacaagtaa taccaatgtc ttcacctttg cctagcaagt
     3061 tgtccggatc aaacattcta aaagccaaac caataactgt cgccaccggg cacaaggtaa
     3121 tatatcgttt gtcccaattc aaacgaactc ccaatatgag ctcaccattg aactcctgcc
     3181 ggcaaacaac acctttatca ggtatagagg ccgcatccga accagcatta ggtcctgtca
     3241 aggcaaagca tgggatctcc ctcccatcgg ccaatcttgg taaataataa tttttttgtt
     3301 cttctgtccc atactttaaa agtaattcac caggccccag tgaattaggt actgaaatag
     3361 ttgtagcagc agtaatagaa cggccataga tttttaccaa aattgacatc tgagcggtag
     3421 cggaaaaacc aagtccacca tactgctttg gaattatcat tcccagaaaa cgattctctt
     3481 ttataaattg ccaaacttcg gggggaagat ctgttctatt atgagtgata tcccaatcat
     3541 ctaacatttc acagagggca ctgacagggc cgtctataaa agcctgctct tcttcagtta
     3601 atgcaacagt tggagcattc ctcaaaagag agaaattagg attaccacta aataaatctc
     3661 cttcccaact cactgtacct gcttccaatg cctctctctc agtaatagac atggaaggca
     3721 tcgctttttt aattatttga aacaaatgtt tcgatagtac ttctcttctt aaaggtttaa
     3781 tggagcctaa aactagtaca gcttcgattg cccataagac gatttgcgac aacaagccag
     3841 gagagccgaa ttgcaaaacc aagccaagaa aaatggtata actaatagcc catacagata
     3901 tcgatgcatg tctggttaat aacgttccag ccacaccaat tgctgctagt attaatataa
     3961 gttctggcac aacattctcc ttattatagg ctaatccaaa tgaaagaatt taatagactt
     4021 ctttctcgat aacaaaatct gcaaaccagt taagacgtct aatcaaatta tagcatcaat
     4081 aacttctata acttgctttc caacagcttt agggtgctcc attggaaata aatgtgtacc
     4141 catagtttta aaacatttta cattaaaatg atttttcata tatcgaatgt ctattctatc
     4201 aactacagta ctcttgtcac catagatcaa tgcggcggga actataagtt tacctttata
     4261 gcgaggaata acatgcggaa ttgttctata tatttgatat tcaatatgcc tgtcaaaacg
     4321 cagatagtat ccgtcctctt tatattccaa accatattta atatagtcat tcaagcattc
     4381 ttctgtaaat gttttaaata aatccctggt ttttaaatag gaaattaatt gttcatagtt
     4441 ttgccaatgc tcacgccttt tctttgttct aaaagctgga gttactctgt ctatgatacc
     4501 taaggcctta gccaatctca ccatgcttga tttaaaagca ccaatgagag gcgaatctag
     4561 cataatgacc gctttaaata attgcggctg ctctatcgca gcgagcaaac tcaaaacccc
     4621 tcccaaagaa tgaccaaccg caatcactgg ctgctctgct tgtcgtttga tgcttgcaat
     4681 gatctcggca accaggttat gccagttttc tccgacagga taatcagggt catgaccaat
     4741 tttatctata aaacaacaat caaaacgagt ttccagctgc tctaataact gtttgtagca
     4801 taatgatgga aatccattgc catgagcaaa atggattaac tctctcattt gagcaccatt
     4861 ttcctgcttt catctcggag accatagaca ccgtaactgt aataggctgc caataatatc
     4921 aacaaaatca ctccaaaccc gggaaaaata aaatgaagcg ataacatgat taccaaaact
     4981 actaacattg gcgttgatgc gaccattaat agccggctgg attttttaaa ggatatagtg
     5041 aacgaaaaaa agattttgga aaatacttgt cctaaaaacg caatgaccaa taaaacagtc
     5101 accataatcg aatataaaat agcaaccact attggatata tcatcaattg agaggcatat
     5161 ttcaaaccat taatcgcatt atcctgaata atttttttgc catcaaatac cacattcatg
     5221 tctttaccta atggctgaac aataggaact ccttgctgtt catttactgg gctgctacct
     5281 aataattctg gtgttggcaa taggaaatta atactatttt ttttaatcag aatattaagg
     5341 taaggatact cctctgtgaa atcagccact ttacctgttg tgtctataat tattaccact
     5401 tgatttttat catttctgat taaatagggc attggtttat caaaaataac attaccattt
     5461 tgtacataaa cagttggcaa ttgcagcaag ggttcaatga tttgctgatt aaacgtttta
     5521 ttaaaatcag aagcaattct tatagaaaac ggtattgaac aaatagcaat aaccaataat
     5581 agataaatca aaccaattcc acgccaatgt tttcctacat caacaaaaag cctctttgaa
     5641 taaaaagaca tatatagtgc ttgccaataa ctatagatag gagcatcaat tggttttaat
     5701 ttattttttt ccttagccac gccactctcc cacacgtttt cctgttctct catcaaatac
     5761 atgcaatttc aatttgggta gctctacatg taattgcctg tttgagacca tttgctctgc
     5821 tgaaatcctc aagttaatct gttcattatt ttttacacat ttcgcctgaa tcaatttatc
     5881 agcccccata tcatcaacaa attctacata aagttcaact gcatcttggc tctgactgga
     5941 acatagttga atgtgttcgg ggcgaattgc tacaataagt tctgctttgt catcgacaga
     6001 atgaagccaa ttaggcgctg aataagttat acctatgtcg gtataaatac tatccgtttt
     6061 cttatcgtat ttgccagaaa taaaattaat aggatagtgc ccggtaaaac cagctacaaa
     6121 ctgcgtagct ggactttgat acagttcttg tggcgttcca atttgctcaa cttccccctg
     6181 attaagcacc agaattctat cagccatagt catcgcttcg gtttgatcat gagtaacgta
     6241 taaactggtc gtattaaatt gttgatgcaa tcgtttaatt tcatgtcgca tttcagttct
     6301 taattttgca tccagatttg ataatggttc atcaaataaa taaactgctg gagaccgcac
     6361 aatagccctg cccattgcta ctctctgctt ttgcccacca gacagtgctt gcggcttcct
     6421 gtccagataa ggagtcaaat gcaataaatt agctactgca gtcactcttt tattaatatc
     6481 tgctttactg aagcgcctca actttaaacc ataagccata ttatcaaaga ccgtcatatg
     6541 aggataaagt gcataatttt gaaacaccat cgccatatcc ctttttgcag cctctgtatc
     6601 attaacacat tgattattaa taagtatttt tcctgaactt aaaacatcaa gtcctgctat
     6661 caatcgtaat aaagtagatt tgccacaacc tgatgggcca acaacaacca tgaattcgcc
     6721 cttctttatt tcaatgctaa ttcgattcaa aatggttgtt tctccgacct gttttgacac
     6781 ctcgatcaaa ttcagtgttg ccattatttc aatccctttt cgaaccaacg ttgcattact
     6841 ataaccacca tacagggtgg cacaagagct atcaatgcga cagccataat atagtgccac
     6901 tccggcactt gatccgctac acctgccaaa taccgaatcc ccatcacaat agttgtcatt
     6961 ttagtttctg tcgtgatgac caaaggccat aagtattgat tccacccata gacaaacaag
     7021 attacaaaca aggcggaaat ttgaactctt gacaggggta acacgatatc aataaaaaat
     7081 cttaatggac ctgcgccatc taatttagca gcatcaacca gttctttaga tatggtttta
     7141 aaaaattggc gaaataaaaa agttccagta gctgatgcca ataatggcaa ggttagacca
     7201 gaaaatgagt tcaataggcc aaatgaagca accacttgaa aagtggggac tattcttact
     7261 tccactggta acatcatcgt tgcaaatatc agggcaaaac aagacttttt aaaaggaaaa
     7321 tcaaaatata ccaatgcaaa agcagatccc agagccaaaa tgatcttacc cactgcaatg
     7381 ataaatgcca tgaataagct atttacaagc atggaagtga taggctcacc accagtcaca
     7441 gctaaacctt ctgtcagcac acttttaata tttttgaaaa atgaggttcc tggaatcaat
     7501 ggcaactgag aatgcatcat gacggttcct tcatgacttg cagcaactac agccaaatac
     7561 aaaggagcca tcattaatcc tataaaaaac aatattaacc catgcgaccc aaaacgttta
     7621 aattgtctca ttcgtaatga acctttttct caaggtattt aaattgtaat aaggaaactg
     7681 caattacaat aatcatcaat aatacagatt gggctgatga acttcccaaa tccattccct
     7741 caaaaccgtc ttcatacacc ttatagatta aattagttgt gctattacca ggacctccgt
     7801 gtgtcatgac ttgaataatt ccaaatgtat caaaaaaacc ataaatcagg ttcattatta
     7861 acagaaaaaa ggtagtgggc gacaggattg ggaaaattat ttgccagaat cgttgccatg
     7921 cattggcccc atccatgatt gctgcatcaa ctaatgtctg aggaactgct ttaagcgcag
     7981 caaaataaaa taaaaaattg taactaaact gctgccaact ggcggttaaa ataacaacca
     8041 gcaaagcttg attcgcgtcg ttgacataat taaaatgaat tcccaaatac tcaagtacct
     8101 gagtcaacca tcctaaagtt ggatggcata aaaaccgcca aagtattgca gcaacagcag
     8161 gggctattgc ataaggccat atcaataaag tcttataaac tccttggctt tttcctctgt
     8221 aatttattaa agtagccatt aaaagaccta aactcatagt gataagcgtc acactaatcg
     8281 caattacaaa agtaacccat atggcttttg cataaccggg atcaaggaat aagtcaaaaa
     8341 aattggtcaa tccggcaaaa ttcttatgca aaccaaatgc gtcagtataa taaaaggatt
     8401 gcaataatgc gctgcaagca ggccaaataa aaaaaataat agtgacaatt agctgaggga
     8461 ctataaatag ccaggcaaac aaacgctgat gattaaattt agacattaac taaccatgta
     8521 gttgttaaaa tgataattat catcgcaata gtttaaaata aaattatatc gattttaaat
     8581 cattatgcga gttgtaaaag atataatata gtaaattatc atactatgca ttcttacctt
     8641 aaaaaggatc ttatcttgca ttacatacag attaataatc caggacctca tagtgaatta
     8701 acccttgccg aaggtccaaa tcctggtttt aatgaatctc aaattcttgt gcgtgtcaaa
     8761 gccacagcct tgaatagagc tgacttgatg caaaaatatg gtaaatatcc ccctcctcca
     8821 ggagaatccg atatacctgg actagaagta gctggggaag taatcgtcac tggttccaag
     8881 gtaaaacaat ttaaaccggg tgataaaatt tacggtcttg tcgggagtgg aggttatgct
     8941 gaaatctgtc cagtagaagc ttctttagct catcatattc cttctcattg gaattattca
     9001 ttggccgcgg cgctcccgga agcgttaacg acagtttatg ctacgttgta cgatttaggt
     9061 gccttaagtg ccggacaaac attattgatc catggcgctg gaagcggaat agcctcatta
     9121 gccatacaaa tggctaaatt aacagcggct aaagtgataa cgacagttgg tgattccagc
     9181 aaaatagaaa aggctcttgc cctgggcgct gatcaaatca ttcattacaa agaacaggat
     9241 tttgaattat taattgagga caattctgtt gatttgatca tcgattttgt cggtggtgat
     9301 tattttaaca aacacttaca tttattgaag cctcagggta aattaatcca aattgcttgc
     9361 ctcaaaggaa gcaaagtaga atgcagtctg gcgttgctta tgcaaaaaag gttgcacatc
     9421 attggatttg tactcagatc gcaaagcatc aaagaaaaag caaggctttg gaaattggct
     9481 catgatcatt ggtttaatgc atttgaaaat gacaagatta aaccagtaat tgactcggaa
     9541 tttgatctaa gtcaaataga aacagcactg caacgcatgc aacaaggctc tcatttcggg
     9601 aaaatagtca ttcgcgtagg taagcaatgc taagcctcat caatttctca tgagctcaat
     9661 attatacgca cagactcttc caaagctgcc ttggcaatgt ccagtgagtt cgtgtttttg
     9721 gtagataaat caagggctct tcttactttt atccttattc cccatagctg aactatcaat
     9781 acatattgag acatttagac tcattttgca ttgagtcgta aagttcgctc taataattga
     9841 gtgtaaatcg gctcgttacg tgctaattgc accacaatcg ttgcagtaat gcaggaaacc
     9901 ataagaggta gaattagaga gtaattttta gtcatttcaa caaccaatac cacgccagta
     9961 attggtgatc ttactgcagc tgcgaaaagc gctcccatac ctgcgactgc aaacatacca
    10021 ggctctattg aaaaatccat acttaaccat tgaaataaat ggaaaaaacc taatccaaat
    10081 agtgttccca aagccagcat tggtgcaaag ataccaccag gcacgccact tgaataggat
    10141 aacatagtca tgataaacct gattacaata agactgcata acacgctaaa tgcaggtgac
    10201 atagtcagtg cttcactgat gataacataa ccgcccccaa cagtagcagg ataggagtac
    10261 gccagataac cgactaaaaa tccaacagct aaaatataat attttttgtt tttggggttt
    10321 tgtttatcta accatccaac agttctcatg agtgtgacat tgaataataa gccagccagc
    10381 cccattaata ttccaaaaac aaagaacagc cacaaagagc ccagtgtagg tagttcaaaa
    10441 acctccattt gaatagccgg ttgtgagcca ataataagat gcaaaacaat cgttgccata
    10501 acacaacaaa taacgaccat tttaaaattg gtaaatgaat aattaaactg gttacgcatt
    10561 tcctccatga caaacagtac accagccaga ggcgcattaa atgctgcagc caaaccagcg
    10621 gcagaaccag cggctattaa agtatcacgg cgacggcgtg taagccgaaa caactctcca
    10681 agcatctgtc caaggttacc acccatctga atagtaggcc cttctcttcc cataaccatt
    10741 tttgccgata tggataacac acccccaaaa aattttactg gtatcagacg cttccaaaat
    10801 attggtctca catgaagcaa ggtaccttca atttcttgca caccacttcc tgccgcttct
    10861 ggggcgatgt acttcaccat catccaggca ataaataaca tcaccatcga tgtcatagcg
    10921 gaaacaattc ctacaggaag tccataactt tctgtcatat gaaataatag ctccagcaga
    10981 tgatccatct gttgaatagt aagttggaat aatgaagcaa ttgcgcctgt taatatccct
    11041 aatagaactg atacaaaata taaaatcagt atttttttac ccattgtcct tcaccatgtt
    11101 catcaaattg atgattattc ttaaacccac ttgatccgat aaagacaaaa ttgtctctgg
    11161 atgaaattga accgcatgca caggtaaata ctcatgagaa actgccataa tcacatcatc
    11221 ttcacttaca gcggtaacta ataattcatt gggtaaagcc tctgctctgg catagagtga
    11281 atggtatctt cccgctttaa aacaatcacc taaaccggag aacaacccct tgttatctaa
    11341 taccctgatt actgaagatt ttccatgcat agggtattca agaacatcca aaacccctcc
    11401 aaaatattca acaattcctt gcagccccag acaaacaccg aatattggta tgttctttga
    11461 gagtatcaca tcgattgttt ctgaaagctt aaaatcaacc ggttttccgg ggccaggcga
    11521 taatacgatc aaatcaaaat gattattttg taaatagcca atcgccttgt catagcgaac
    11581 tgttgtaacc gcagctcccg tttgtcttaa atagtttgcc aaagtatgaa caaaggaatc
    11641 ttgatgatcg ataagtaaaa cttttttatt tttgcctacc aggggaacat cttctgtctt
    11701 ttttttagcc ttttgccatg gtttttgtaa aatgtctaaa aaagcagaag cttttaatct
    11761 cgtctcctct tcctcagctt cagggataga gtcataaagt aacgttgcgc ctactctaat
    11821 ttctgcagtt cccttttcta ttcgcactgt tcttaagacc aatccagtat ttaaatttcc
    11881 atcaaaacca aaccatccta cagcccctgc ataccactta cgtggggatt tctcatgttg
    11941 ttcaatgaaa ttcatagccc aaattttagg cgcaccagtt acagtaacaa cccacatatg
    12001 agttaaaaaa gcatctactg catcaaatcc atctcttaaa ataccctcaa catgatcaac
    12061 tgtatgaatc aatcgggaat acatttcgat ttggcgacgc ccaatgactt tcacactccc
    12121 tgcttcgcat attcttgact tatcatttct atccacatcg gtgcacatgg ttagttctga
    12181 ttcttctttc tctgaatcta gaagtgtttg aatattgtga gcatcctcta tagcatctgc
    12241 tccccgtttg atagtgcctg agatagggca agtttctact cttttacctt ggacacgaac
    12301 atacatttca ggtgaagcac ctaccagata ttcttcgtca cccaaattaa caaaaaatcc
    12361 atatggcgat ggatttattt ctcgcatttg gcgaaataat attgacggct tttcagcaaa
    12421 gtgagtatag aaagtttggc taggaaccac ttcaaataaa tcaccacagg aaaatttttc
    12481 tttggctttt ttgaccactt cggcatattc acctggctcg tgatcacagt ttttttccgg
    12541 tttatggata ggttgatatg gtgaatactt cccttctctg gataaagatt gagttgatcg
    12601 cccctgatat tgaaaatcat agcgtctaac aaaggcttct tccttacgat gattcactat
    12661 ataaatttca tcaggtagat agagaaccat atctctctgt gtttcatttc ttggtttaca
    12721 ccgctccaac tgttcaaatt gataaatcag gtcaaaccca aaagcaccat ataaacccaa
    12781 gtatgaatct tcttctgttt taaaaaaatt caataagagg cgaattgcac tgaatactga
    12841 cggttgatgg ctccgctcct cctcactaaa tactttgtct gatgatttaa tttttaactg
    12901 acaaaatgag gtcgtttggc taattacctc ccagagatct gttgttttta acaacggata
    12961 aagaaaagat aataatatct cgccccgttg atttaatgcc tcgaaacata tggtattatt
    13021 ttgacagatc ataactaatg ggggattata aaaacccatg tcccaacaag tatagcgtcc
    13081 cggatattca aagctcgaag caaatactgc tccacgttga gaatcaagac tctcaagcaa
    13141 aaaatctatg ccttgttgat aatccaactc ttgttgagaa aactcaacat gaacaccacc
    13201 gagagtttta tattgttgta acatgaatac cattctctat catttaattc gtacaaatat
    13261 tcattctact gtaaaaccac tgttctgcaa gaatcttgta acatttcaat atcattgctt
    13321 ttttatgtct tcagaagaga ttgcaaaact tgcttcaaaa aagacatgac tgcagtcatg
    13381 attaaacatg gctccaggaa agcaacttgg ctcaacagac taaaagataa ttaaactaat
    13441 acttcatttt tagaacaata ccgctataat gtataaccct tatatttgga tatgaccatg
    13501 actctctctg ctaaagatct tgaaaatatt tcaaaattgg cttatttaaa tgaagactct
    13561 gagcattctg caaaattgac tcaagaagta agtgcgatca tggattttgt tgaccagctc
    13621 aaaacaatag atacaagcca aatagcacct ctttttcatc ccttggcact aaatcaacgc
    13681 ttaagaccag atgaagtaac tgaagcggaa tgtcttgctg aattagaagc catggcacca
    13741 ttatttgagg ataacttata tttggttccc aaggtgatat catcggataa ataataatgg
    13801 aacattactc actggctcaa ttatcaaagg ctcttcataa tcgagaattt tcaagtgttg
    13861 aattaacaca acactgtatt aacaaaattc aaagcaataa agacttgaat gcattcatca
    13921 gccttgatga agaccaagct cttaaagaag cgcaatcggc tgatttggta ctaaaaaatg
    13981 gagagggaaa gccgctgact gggattccca tggctctcaa ggatttgttt tgtacaaagc
    14041 gattaaatac aacttgtgct tcaaaaatgc tggctaattt tcaagccccc tatgatgcca
    14101 ctatcgtcac aaaatttaaa caaaatggcg caataattat tggaaaaacc aatatggatg
    14161 agtttgccat gggatcctct aatgaaaaca gttacttcgg ttcggtaaaa aacccgtggg
    14221 atagagaaag agtgccaggt ggatcatcag gcggttctgc agcagcggtt gctggaaatc
    14281 tggtaccttt tgcaattggc tctgacactg gtgggtcaat tcgtcagcca gcagcctttt
    14341 gcgggattag cgggataaaa cccacctatg gattagtctc tcgatacgga atggttgcct
    14401 ttgcctcaag ccttgatcaa gcaggtccat ttgccagatc agctgaagat cttgccatga
    14461 tacttcactg tatggcaggg tttgataaca aagattccac gtcagttgat agagttatac
    14521 ctgattattc agcagaaatt aaaaaaccag tagacaaaat acgcatagga cttccttctt
    14581 gtttttttca acctcaagtc gaaaaaggta tccaggatgc catacatgat gctgtgaaat
    14641 tatttgagaa tctcggggct gaaataattg aaatcgattt gaaacttcag ccactttggg
    14701 tgccatgtta ctatgttata gcttgcgcag aggcttcatc aaatctttcg cgttatgatg
    14761 gcataagatt tggccaccga agcaaatccg catcaacctt aatcgaatta atcactaact
    14821 ctcgaagtga aggttttggt aacgaagtaa aacgtcgaat tctaactgga actcatgtgc
    14881 tctcaaccgg tttttttgat gcctattatt tgcatgcaca aaaagtgagg cgtttgattc
    14941 gagatgagtt aattacaact ttaaattcag tagatgttat cattgggcca acgaccccta
    15001 ccaccgcttt taaattgggt gaaaaaataa atgatcccat acaaaattat ttggcagatg
    15061 tatttactgt agcagccaat cttgcaggcc tgccagctat ttctatacca actggttttg
    15121 aaaataaact accaattggt ttgcaattaa tgggtaagca ctttagtgaa agccgcttgc
    15181 ttgcaatcgc acatcattat cagcaacaca ccaactggca tttagccaat cctaacaagc
    15241 aagggtgata tatggaatgg gataccgtca ttggtcttga ggtacatgct caattaaaaa
    15301 ccaaatcaaa attattttca ggagcatcta ctgcttttgg tgccacccct aactcgcaaa
    15361 cgagttttat cgatgctggt ttgcctggtg ttctccctgt attaaacgaa caagccatca
    15421 taatggctat ccaatttggt ttggcaattc atggaaccat taatgatcta tctgtttttg
    15481 agcgtaaaaa ttatttttat cctgacttac ccaaaggcta tcaaatcagt cagtatcaaa
    15541 aacccattgt tactaatggc tatttaaata tacagcttgg taataatctg gaaaaaacag
    15601 tccatattgc acgcgctcat ttagaagaag atgctggcaa atccctacac gatgcccata
    15661 ctgattacac cgggatcgac ctcaacagag caggtacccc actattggaa atagtcacaa
    15721 ccccttgcct gcactccgct gaagaggcga ttaattattt aaaaacgctg catcaattag
    15781 tgagattcct tggaatatgc gatggcaata tgcaagaagg ttcttttcgg tgtgacgtca
    15841 atctgtctat aaaacctaaa ggcagctcag ttttaggcac acgaacagaa cttaagaatc
    15901 tgaattcatt tcgttttatt gaaaaagcca ttgcttttga acaggcaaga caccaagata
    15961 tactggaaag cggtctgtca gtcatccaag agactcgcct ttacaatcct gacaataaca
    16021 caactcaggc aatgagaggc aaggaaaatg aaaatgatta ccgttatttt cctgatcctg
    16081 atttattacc aattcatatt gacaaagaac aaattgaagc gattaaaaat aacctgccgg
    16141 acttgcctga agcaatttcc aaagagttaa aaagcactcc gtccctaaat gatgaagaca
    16201 ttaatttcat attatcatca ccggacactt atcaatatta caaaaaaatt aaatcccttt
    16261 gtccggctgc tgataaaaca attatcaact ggttaaaagg ccaatatgcc gcctttctta
    16321 atgaacacaa cctaaccttt gaaacaccac caatttcagc taaaaccatg gctgctttcc
    16381 ttagcaaaat tcatgagaaa aaaatctctt ccagtattgc taaaaatatt ttcagtatgc
    16441 tttgtgtagg tgaagaagat atcgatgcga ttattgaacg cgaaggctac cagcaacaga
    16501 atgacaaccc ggcactggaa gaaatagttg agcaaataat caaacaatat cccgaccaag
    16561 ttacagaata caaagccggt aaagagaaat tattagcttt ttttatcggg caagcgatga
    16621 aacaaactaa agggaaagca aatcccgagc aaatcaatct gttattaaaa aaacatcttg
    16681 gatgatgcaa tatttttata attcacaatg atcatttatg gctttgttga ttgattgcca
    16741 gctattatca tctttttgtc agaaagtata atgcattaag aataataatc tttcaattat
    16801 a
//
LOCUS       DASLOE010000052        16773 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.052, whole genome shotgun sequence.
ACCESSION   DASLOE010000052 DASLOE010000000
VERSION     DASLOE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16773
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.052"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            227..883
                     /locus_tag="U6309_001374"
     CDS             227..883
                     /locus_tag="U6309_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504823.1"
                     /translation="MPQRQSEIVVLKPTNLFLSFLASQLPEANLPSLKLLHTDNTAYV
                     IPKHDSDDGTLNEIEKHFTTMFRHEICRWLGRSAHNEIETSFLDFLCCFKFELHSHII
                     LMEPSLKEGHQMLNIKPRSALLEWMKCAVEDQEGLSDVMSRVNLAQIAENSTVIVKNF
                     TTIKDVKPFIKQYFKPIFETTMSRISGQSVQWPQVNSFQSFSRYFAVEIHTQLIHLHY
                     "
     gene            894..1790
                     /locus_tag="U6309_001375"
     CDS             894..1790
                     /locus_tag="U6309_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504824.1"
                     /translation="MEPVTIALICAASFGAVVALSAFIRQLLLSRDKNLNDEAQRKAI
                     NQEAGELEKMREQMQSNKRFDSHYKVLGANKDAIIYLDNKIEDILRKKAHLVERYAQV
                     SLKESGAIVEGGISTERKDACDRLKKEIDEEIKFYDSELLHLQQRRTSLWDTHTDLQE
                     YLLGQEKSRNESLDHLYKQHTALLEKIYIRHTDDSENIAKQSIDAGTTSFKAIILAPI
                     QFLLQYFNISSGINFDQSKLENAARDDVDKTESDINDSENSDDEIYSDTDANSDDESN
                     RSDNEDSRDESDSENDSRLIFA"
     gene            2006..2344
                     /locus_tag="U6309_001376"
     CDS             2006..2344
                     /locus_tag="U6309_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504825.1"
                     /translation="MVYIKRDKDGRICAVYNQKTSDVTEQIDINDAEFVDFLLRCESD
                     EKLKFLQSDLQLIRVLEDLIDVLIDKNIITITDFPQSVIDKLLARQAFRKRFASRAGM
                     ELNGDDETTT"
     gene            2319..3410
                     /locus_tag="U6309_001377"
     CDS             2319..3410
                     /locus_tag="U6309_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEN5504826.1"
                     /translation="MEMMRQQRNLLLIPFILCFILARPVFSLNLDALLPDERNTVEVF
                     QKASSKVVYVHRLANATVQRRYSLQKTHIPDGAGSGIIWDNKGHVVTNFHVINGADDI
                     AITLGNMTVPAKVIGSEPRKDIAVLEIKSPKALNYLKSFQPFEIVSLNDLIVGQKAIA
                     IGNPFGLDHSLSKGVISALGRKVPGIGGVTIYDMIQTDTPINPGNSGGPLLNSAGQLI
                     GMNTMIYSRSGSSAGIGFAVPAEDIQKIASQIINHGRVVLSGIGIQRVEPHLAERLGV
                     KKGILIADVVPGTPADKLKLRGTHRDQWGRIVLGDVIVGVNAHPVPNYDALYNLLTEI
                     KVGEQITVSIIRHGKKMDVKMKTIDIAAL"
     gene            3530..4075
                     /locus_tag="U6309_001378"
     CDS             3530..4075
                     /locus_tag="U6309_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN5504827.1"
                     /translation="MLLNRADSLLVLIDVQEKLTPAILNLDLFLARCEWLLKLARKLD
                     VPVLASEQYPKGLGRTVSPLSSYINQEECIEKIHFSCMQEPQYINLLKGYNKNQLILI
                     GIEAHVCVLQTAIEMKGYGFEVFVVVDGVSSRSEFDLKYGLKRMKQNGIHLITAEMVL
                     FEWLKQAGTPEFKAISKEFLQ"
     gene            4284..5027
                     /locus_tag="U6309_001379"
     CDS             4284..5027
                     /locus_tag="U6309_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN5504828.1"
                     /translation="MNFDNQIALVTGGVSGMGKACVQYLQQHGMKVVVWDKQEGTSNE
                     AELYVACDVTSDESVEKAMRQTISQVGVPRVCINCAGIAPAKRMVGKEGPMPLESFRQ
                     VIDVNLIGTFNVMRIAAHAMSGLELDNKSQERGVIINTASIAAFEGQIGQSAYSASKG
                     GIVSMTLPAARELAQFAIRVNTIAPGLIATPLLLNMPQEVQDSLVATVTFPKRLGRPE
                     EFASLVGHIIENQMINGEVIRLDAALRMR"
     gene            complement(5090..5587)
                     /locus_tag="U6309_001380"
     CDS             complement(5090..5587)
                     /locus_tag="U6309_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HEN5504829.1"
                     /translation="MNNNNKAKTLITQLEQEISWVEELNTLLSEEKEILSTSQFNQLE
                     TLAEKKQELSTKLEASAKQRVDLIKESNPGDKPHGLLLSDFLKNCATEDGIQINHLNN
                     TLAEKLNLCRELNTINGQVIANNMYTRQQIVNALSGNKSNAVSVYTSNGNLETPSESS
                     HHEEA"
     gene            complement(5604..5924)
                     /gene="flgM"
                     /locus_tag="U6309_001381"
     CDS             complement(5604..5924)
                     /gene="flgM"
                     /locus_tag="U6309_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEN5504830.1"
                     /translation="MINTIEDIHMVNQINDSANLRHIDMDNRINANHKEVKSPVLENN
                     STDSVNLSSTSKQLEALKASLKDLPEINEARVLYFKAEIQSGQYEIDSSKIAHGMLNS
                     VEMV"
     gene            complement(6015..6716)
                     /gene="flgA"
                     /locus_tag="U6309_001382"
     CDS             complement(6015..6716)
                     /gene="flgA"
                     /locus_tag="U6309_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HEN5504831.1"
                     /translation="MKKCILSFFLFFASTSLFSESSQSLELLKNKIEQYALNELSNYT
                     EGKIQVTADNIDPRLNLKVCDDNKLEVFNPYQTPMLNTSTMGIKCLEENNHWTLYIPV
                     KITIFKSVLVAKRALLKGTKISNSDIYQTELDVQKLKQGYFTDSKELIGLVCKHDITP
                     NSPLNPFNIELAKLVHKGEQVSIIAAHDNLTVSMDGIAMDEGALGDSVKVKNLSSKRI
                     IEAQVTGKKTVKVIF"
     gene            6803..7210
                     /locus_tag="U6309_001383"
     CDS             6803..7210
                     /locus_tag="U6309_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEN5504832.1"
                     /translation="MRPCYFVCILALFMFAMPGYSENHHPQEFLKSIAGTKDEGEKIF
                     NHFCVNCHGTKPMISLGAPEIGNERAWGARLKQGMEVLFKHTDEGFNAMPPRGGCFEC
                     TDKQLILAIIAMVPKQAQKGLLNNLLDHKEYKQ"
     gene            7287..7844
                     /locus_tag="U6309_001384"
     CDS             7287..7844
                     /locus_tag="U6309_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEN5504833.1"
                     /translation="MKKQILLVPVCVLAASCASMDRSTAIVDDAGYTHYTMSMESDRK
                     GSNYFPEKRQATGKKVFIFDPKATAWAAYDAQGNRVKTGSASGGKDFCEDVGRPCRTV
                     TGSFKVYSKKGEECTSSIYPIETGGGAKMPYCMHFHGGYSIHAAYEVPNYNASHGCIR
                     VLPSAAKWLNQNFIDVGTSVIVKPY"
     gene            8113..8883
                     /locus_tag="U6309_001385"
     CDS             8113..8883
                     /locus_tag="U6309_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEN5504834.1"
                     /translation="MILCIDVGNSHIYGGVFDGDEIKLRFRHTSKVSTSDELGIFLKS
                     VLRENNCSPETIRKIAICSVVPQVDYSLRSACVKYFSIDPFLLQAGVKTGLNIKYRNP
                     VEVGADRIANAIAATHSFPNQNIIVIDFGTATTFCAISHKKAYLGGAILPGLRLSADA
                     LSKNTAKLPSVEIIKTESVVGRSTIESIQSGVYYGVLGACKELIQRIHHEAFNGDKIL
                     ILATGGFASLFDKQGLYDHLVPDLVLQGIRLAAMMNTA"
     gene            9496..9954
                     /gene="mraZ"
                     /locus_tag="U6309_001386"
     CDS             9496..9954
                     /gene="mraZ"
                     /locus_tag="U6309_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEN5504835.1"
                     /translation="MFRGINAITIDTKGRLAIPTRYRSALGAEDKIPLVVTIDTEETC
                     LLLYTAAQWQIIEDNLQKLPSFNAAARRIQRLLIGHATDVEVDANGRVLLPTVLRNYA
                     KLEKDVVMIGQGNKFEVWNKELWESKREQWLAEEASMTDGLPEEMKTFSL"
     gene            9966..10892
                     /gene="rsmH"
                     /locus_tag="U6309_001387"
     CDS             9966..10892
                     /gene="rsmH"
                     /locus_tag="U6309_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEN5504836.1"
                     /translation="MAKHQSVLLHESIKGLAIKADGIYFDGTFGRGGHSREILNHLSD
                     KGRLFAIDKDLDAVQYAKDYFGLDKRFQIFHGSFAQIKEFASQAGVIGAVDGILLDLG
                     VSSPQLDNPERGFSFMLQGPLDMRMDLTQSINAANFVNEAEVNELAHVFRAYGEERFA
                     GRIAKAIVDARKLKPITTTLELAEIVKEANPKWEKHKHPATRVFQAIRIHVNQELTDL
                     SNCLEQCLDVLGPRGRLAVISFHSLEDRIVKQFMRDKEQGNRPPVEVPIKYEELKTNF
                     KRVGKAVKPQSSEIKENVRSRSAVLRIGEKLA"
     gene            10889..11227
                     /gene="ftsL"
                     /locus_tag="U6309_001388"
     CDS             10889..11227
                     /gene="ftsL"
                     /locus_tag="U6309_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEN5504837.1"
                     /translation="MNAAAKVINQGNLFNGQLADMQMSKSLYMLIVLLAAVLVSAFAV
                     IYSTNSYRLTLSQVQQEEQQTHFLQLQWGQLLLEQASLATPARVEELAREKLGMTLPT
                     SANTYLLHPQ"
     gene            11609..13270
                     /locus_tag="U6309_001389"
     CDS             11609..13270
                     /locus_tag="U6309_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEN5504838.1"
                     /translation="MKKTHHFARLVTVAVFFSLILAVLIWRMVDLTVLHRQFLQGQGD
                     ARSIRVIDIPAYRGMITDRNGTPLAVSTPVESVWVNPKEFSPDEEQFMQLAKYLNLAP
                     KELSRKIVRAENREFVYLQRQLPPPLSKKIEALKIPGVNFQKEFKRYYPDSDSISPLI
                     GFTNVDDQGLEGIELAYQDWLMGVVGKKRVIKDRLGRVVEDLGVLKEPRPGHELTLSI
                     DRRLQYLAYSELGKTVEEFSAKSGSVVVIDAENGEILAVANVPSYNPNARGHYDKETY
                     RNRAFTDTFEPGSVIKSFSIASALETGLFTPDTIIDTNPGWMTVHGRTVRDIHNYGVL
                     DVTGILQHSSNVGVTKMVLSTPAEQLIGFLQKCGFGQRSELAYPGESEGGIVKAKDAN
                     PFVLATLGFGYGLSVTALQLAKANLIFANKGRLIPVTLIHNDTPSNGIQVMQPKTAEN
                     VLLMMEAVMNKGTGKGARVPGYRVAGKTGTARVAGKNGYKDRKYTASFIGIAPVSKPK
                     FVVAVIIHEPSRKGYYAASVAGPLFAKVMSGALRLFNIPTDEPVS"
     gene            13292..14743
                     /locus_tag="U6309_001390"
     CDS             13292..14743
                     /locus_tag="U6309_001390"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEN5504839.1"
                     /translation="MKLTQLLEPWIKRDIIDGTVSDIKNDSRLVKEGDLFVAYPGAAS
                     DGRLYIEKAVASGAVAVVYEPNNLPQGITLPTAIPCIAIPQLAEKLGEIAKRFFNNPS
                     QFLGLTGVTGTNGKTTIAYQLAQAHDLLGQHSAYIGTIGQGKVDALKPLENTTPDALC
                     LQRLMHQYKEEGIKQVCMEVSSHALSQHRVDSLEFSQAIFTNLTLDHLDYHQTMQAYG
                     AAKSRLFERAELQWAIINQDDDFQDLMSNVLKTHVKKVTYGMHQNCDVKALQWNMDIT
                     GTEIEVKSPWGHQLLKIKALGKFNIYNSLAVYSSLLASGHNPEKVVQVMAELKAAPGR
                     MEIVAQSPYVLVDYAHTPDALENVLITLKQIKKGRLWVVFGCGGDRDKTKRPIMGKAA
                     SMYADNIVITSDNPRSEDPEIILNEIACGIPASAPVSKLVNREEAIAYALRKAEHNDI
                     ILIAGKGHESYQQIGNTKHVFSDQEVVRRLMNN"
     gene            complement(14788..15840)
                     /locus_tag="U6309_001391"
     CDS             complement(14788..15840)
                     /locus_tag="U6309_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HEN5504840.1"
                     /translation="MNILADALLPGLDSAFPPPFTVTLYHKADEIPELLHHKDVLLCR
                     STLKINGDLLKNHQIKVVATATSGTDHIDFPFLESQNISIIDAKGCNAISVADYVVAC
                     LAYLDKQQLIQGKTAGIIGLGQVGTKVYERLNAAEFQLCLYDPPKATRDTSFQSCSLE
                     DLFECDLLCVHAELHSDAPYPSLNLINRDFLKELKPGCIIINASRGGIVNEEALLHLG
                     SAILYCTDVYNNEPHIDSRIVSKATLCTPHIAGHSLEAKFAAVAIVSRKLHQMLGLPY
                     PQFATPEKPYRLNENSDWRELALSIYNPIHETLELKHAGNLSSAFLTLRKNHHHRHDF
                     TTYFDSDSIKKYPLLG"
     gene            complement(15837..16496)
                     /locus_tag="U6309_001392"
     CDS             complement(15837..16496)
                     /locus_tag="U6309_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEN5504841.1"
                     /translation="MPEFTLIQQICIWALPVLLAITFHELAHGYVAYRCGDNTAKMFG
                     RLTLNPIKHIDPIGTVLLPLLIGVITNFNFVIGYAKPVPVNQSQLRKPRRDMILVTLA
                     GPLANLIMAFLWAGCFKIATFLNPQSSMAALFLLVTARAGMMINLILAFLNLIPIPPL
                     DGSKVVINLLPARQAIAYAKLEPFGFLILIVLIFTNVLGYILTPLIYGSLYILSAIFN
                     L"
BASE COUNT         5220 a         2820 c         3572 g         5161 t
ORIGIN      
        1 gttaatgtat taccagaatt ttggttgatg tacttaatct tggttctaaa tttaacaata
       61 tcagggaatt tttaagtctt tgaaagtcaa gtgtgacttc atgcacattt gtgaataaaa
      121 ctctgtatta gtcaagatag tgactaagat tctaaattga gatgagacgt tgattttaat
      181 agttgagatg taatcaggag gagttaatta aatcaaggag agcctcatgc ctcaaagaca
      241 atctgagata gtagtgttga agcctactaa tttattttta tcctttctag cgtcacaatt
      301 accggaagct aatttaccca gcttaaaatt attgcatact gacaatacgg cttatgttat
      361 tcccaagcat gattctgacg atggtactct taacgagatc gaaaagcatt ttactacaat
      421 gttcagacat gaaatttgtc gttggttggg gcgttctgca cataatgaaa ttgaaacaag
      481 ttttcttgat tttctgtgtt gttttaaatt tgagttacat tctcatatta ttttaatgga
      541 accctcttta aaagaagggc accagatgtt gaatatcaag cctcgttcag cgcttttaga
      601 gtggatgaaa tgtgctgttg aagatcaaga aggcctttct gatgttatga gtcgggtgaa
      661 tttggctcag atagcggaaa actcaacggt tattgttaaa aacttcacaa ctatcaaaga
      721 tgtaaagcca tttataaaac agtattttaa gccaatattt gaaacgacta tgagccggat
      781 aagcggtcaa tcagtacaat ggccacaagt caattcattt caatctttca gccggtattt
      841 tgctgttgaa atacacaccc aattgataca tttgcactat taaggagttg actatggaac
      901 cagtgactat agccctcatc tgtgcggcgt cgtttggagc agttgtagcg ttatccgcat
      961 ttatcagaca gctattatta agtagagata aaaatttaaa tgatgaagca cagagaaaag
     1021 caataaatca ggaggccggt gaactcgaga aaatgagaga gcagatgcaa agcaataaac
     1081 gatttgattc tcattataaa gttcttggcg cgaataaaga tgcgattatc tacctggata
     1141 ataaaattga ggatatttta cgtaaaaaag ctcacttagt ggagcgttat gcacaagtat
     1201 ctctcaagga atcaggggcc atagttgagg gtgggatctc gacagaaaga aaggatgcct
     1261 gtgacaggtt aaagaaggag atagatgaag aaataaaatt ctatgatagt gaattgctgc
     1321 acctgcaaca aaggcgaact tcattgtggg atactcatac ggatttacaa gaatatttgt
     1381 tgggtcagga aaagtctcgc aatgagagtc tggatcatct ctacaagcaa catacagcac
     1441 ttttagaaaa aatatatatt cggcatactg atgacagtga aaatattgca aaacaaagta
     1501 ttgatgcagg aaccacctca tttaaggcga ttattttggc gcctatacaa ttcttgctgc
     1561 aatattttaa tatttcatca ggtattaatt ttgaccaatc taaattggag aatgctgcgc
     1621 gagatgatgt tgataaaact gaaagtgata tcaatgattc tgagaatagc gatgatgaga
     1681 tttatagcga taccgatgcc aattctgacg atgagtctaa tcgttcagat aatgaagact
     1741 ctcgggatga gtcggattct gaaaatgatt caagactaat ttttgcataa aaattaatct
     1801 tttgagttaa ttggtgacat ctatgaaagg atatattaca atacaatgct tgtacttaat
     1861 gatattcctg gaataattca tgaaagtctt ttgtctgggt ttgggcttac cattaacatg
     1921 gaaggtttga gtttagagac agcagacata agatttatgt gtctgttttt tctaaacata
     1981 gtattagcta aaaaaggatt aaacagtggt ctacataaaa cgagataagg atggtcggat
     2041 ttgtgcagta tataaccaga agacctcaga tgtaacggag caaattgaca ttaatgatgc
     2101 agaatttgtt gattttttgt tgcgttgtga gagcgacgaa aaattaaaat ttctacaatc
     2161 cgatttgcaa ttgattcgtg tacttgaaga tttgattgat gttttaatag ataaaaatat
     2221 cattactatt acagattttc cacaatcagt aattgataaa ttattagcgc gacaggcatt
     2281 tcgcaagaga tttgcaagcc gtgcaggtat ggagttaaat ggagatgatg agacaacaac
     2341 gtaacttatt acttatccct tttattttat gttttattct ggcaagacct gtttttagtt
     2401 taaacctgga tgctctcctt ccagatgaac gaaatacagt tgaagtattt caaaaagcct
     2461 catccaaagt tgtttatgtt catcgattgg caaatgctac tgttcagcgt cgttactcat
     2521 tacaaaaaac acatattcct gatggggcag gctccggtat tatttgggat aacaaaggcc
     2581 atgttgtaac caattttcat gtcattaacg gtgctgatga catagcaatt actttgggaa
     2641 atatgactgt ccctgccaaa gtgattggtt cagagcctcg caaggacatt gcagtccttg
     2701 aaattaaatc acctaaggct ctgaattatt tgaaatcatt tcagcccttt gaaatagtct
     2761 ctcttaatga tctgatcgtt gggcaaaaag cgattgcgat tgggaatcct tttgggttgg
     2821 atcatagttt atcaaaggga gtgatttctg ctttaggtag aaaagtacca ggtataggtg
     2881 gggtaacaat ttatgatatg attcagacag atacccctat caatccgggt aattcaggag
     2941 gtcctctatt aaatagcgca ggccaattaa ttggtatgaa caccatgatt tattcacgat
     3001 ctggttcttc tgcaggtatt ggtttcgcag ttcccgcgga agatattcag aaaattgcat
     3061 ctcaaattat taatcatggt cgagtggtat tgtctggtat cggtattcaa cgagttgagc
     3121 cacatcttgc ggaaaggctg ggagttaaaa agggtatttt gattgctgat gtggtgcctg
     3181 gaactcctgc agacaaattg aagttacgag gtactcacag agatcaatgg gggcgtattg
     3241 ttttagggga tgttattgta ggtgtgaatg cacatcctgt cccaaattat gatgccctgt
     3301 ataatttgct cacggaaatt aaggtgggag agcaaattac tgtttcaata ataaggcatg
     3361 gtaagaaaat ggatgtcaaa atgaaaacaa ttgatattgc agcgctttaa ctctcttcat
     3421 tttttctaac tattatttcc ccggaaaaga attatgcctc aatccggggt tcactcactt
     3481 ttcttaatgc tatatgatat agatcaataa atagaatgag gctatcagga tgttactaaa
     3541 tagagcggat tctcttttgg tcttgattga tgtacaggaa aaactaaccc cggcaatatt
     3601 aaatctcgat ctttttttag ctcgatgtga atggttacta aaattagcca gaaaactgga
     3661 cgtgcctgtt ctagctagtg aacaataccc taaaggttta ggtcgaaccg tttctccttt
     3721 aagctcttat atcaatcaag aagaatgcat tgaaaaaatt catttctcat gcatgcaaga
     3781 accacaatat attaacctcc tgaaaggata taataaaaac cagcttattc ttatcggaat
     3841 tgaggctcat gtatgtgtgc ttcaaacggc catagaaatg aagggctatg gattcgaggt
     3901 ctttgttgtt gtagatggtg tgagcagtcg ctctgagttt gatttaaaat acggtctaaa
     3961 aaggatgaaa cagaatggga tccatttgat aaccgctgaa atggtactgt tcgagtggtt
     4021 aaaacaagca ggtacgccag aatttaaagc gattagcaaa gaatttttac aatagaccat
     4081 ttgcatggcc tgattatttc cttttattgc tgtgttgcaa acgtttctgc ggtactcatg
     4141 tactgtatgt aagcttcctt cctcaaagcg gttttgtgct ttgccttaaa aaataagcag
     4201 gtttgccaag aggtctaata gaatactgtt tttaagtaga ataagaaagt tggaaattga
     4261 aaccgtagaa ttttggagag aaaatgaatt tcgataatca gattgcacta gtaactggtg
     4321 gtgtctcagg tatgggtaag gcttgtgtac aatacttaca acagcatgga atgaaagttg
     4381 tggtctggga taaacaggaa ggcacctcaa atgaggcgga attgtatgtt gcttgtgatg
     4441 taaccagtga tgaatctgtt gaaaaggcaa tgcgacaaac tatctctcag gtaggagtac
     4501 caagggtttg tatcaattgt gcgggaattg ctccggcaaa acgcatggta ggaaaagaag
     4561 ggccaatgcc tttggaatca tttagacaag taatagatgt caatttaata ggtaccttca
     4621 acgtcatgcg aatagctgca catgccatgt caggattgga gttagacaac aaatctcagg
     4681 aacggggtgt cataatcaat acagcttcca ttgctgcttt tgagggacaa ataggacagt
     4741 ctgcttatag tgcgtcaaag ggaggaatag tatcaatgac cttgccagca gcacgtgaat
     4801 tagctcaatt tgcaataaga gtaaatacaa ttgcgccggg gttaatagca actcctttac
     4861 ttttaaatat gcctcaagaa gttcaggaca gtttggttgc aacagtgact tttcccaaac
     4921 gcctgggcag accagaggaa tttgcttcac tagtaggtca tattattgaa aaccagatga
     4981 ttaatggaga ggtaattcgt ttggatgcgg ctctccgtat gcgttgagcg gctatgcctg
     5041 gcgcatcata ctggagagct attttaaaaa atactagggg ttatatttct tatgcttctt
     5101 catggtgact gctttcggaa ggggtttcca aattcccgtt agaagtgtaa acactcacag
     5161 cattagattt atttcccgac aaggcgttga caatttgttg acgggtatac atgttattcg
     5221 ctatgacttg tccattaatg gtatttaact cacgacatag gtttaatttc tctgcaagag
     5281 tattatttaa atgattgatt tgaatgccat cttctgtagc gcaatttttg agaaaatcag
     5341 acaacaggag tccatgaggt ttgtcgccgg gatttgattc ttttataaga tctacacgtt
     5401 gttttgcact agcctctaat ttagttgata attcctgttt tttctcagct aatgtctcca
     5461 gctgattgaa ttgactggta gaaagtatct ctttttcttc agataataaa gtattgagtt
     5521 cttctaccca gctaatttcc tgttctaatt gggttattaa tgtttttgct ttattgttat
     5581 tattcatcgg gtcgccctgc agtttaaacc atctctacac tatttaacat gccatgtgca
     5641 atcttgctgc tatcaatttc atactgcccg gattgaattt cagctttgaa atacaataca
     5701 cgagcctcat tgatttcagg taaatcttta agagatgctt ttaaagcttc cagttgcttc
     5761 gatgtactac ttaaattgac gctatcagta gagttatttt ccagaacagg gcttttgact
     5821 tccttgtgat ttgcattgat acgattgtcc atatcgatat gccttaaatt ggcagagtca
     5881 ttaatttggt taaccatatg aatatcctca atcgtattta tcatgattat cggctccttt
     5941 tgcaatttct ttagtaaatt aaggttgatt tttaatcctt tttccaaatt ttttcttaaa
     6001 aattaaaaca ttagttaaaa aataactttc acggttttct ttcctgtgac ttgagcttca
     6061 ataattcgtt ttgaggaaag attttttact tttacagagt ctcctagcgc tccctcatcc
     6121 attgcaattc catccatact cactgttaag ttgtcatgag cggctattat agatacctgt
     6181 tctcctttat ggaccagttt ggctaattca atattgaaag ggtttagtgg gctattaggc
     6241 gtgatatcat gcttgcaaac caatccaata agctctttac tgtcagtaaa atatccttgt
     6301 ttcagttttt gaacatcaag ctcggtttga tatatatctg aattactaat ttttgtacct
     6361 ttcagtaaag ctcgttttgc cacaagtaca gatttaaata tagttatttt gacaggtata
     6421 tataaggtcc agtgattgtt ttcctccaaa catttgattc ccatggtact ggtattgagc
     6481 atgggtgttt gataagggtt aaatacttca agtttattat catcacaaac ttttaagttt
     6541 aggcgaggat caatattatc cgccgtaacc tgaattttac cttcagtata gtttgataat
     6601 tcattgaggg cgtattgttc tattttattt tttaataatt cgagagactg acttgattct
     6661 gaaaataaac tggtgcttgc aaaaaataag aaaaagctta gtatgcactt tttcacgaca
     6721 taaactccct tagtctattg attttttata ttgaaacgca gcgtgtccca agcaagtatt
     6781 gcgccaattt taggagtata tcatgagacc ttgttatttt gtttgtatat tggctttatt
     6841 tatgtttgcg atgccagggt actctgagaa tcatcatcct caagagtttc taaagtcaat
     6901 tgctggcaca aaggatgaag gggaaaaaat ttttaatcat ttttgtgtta attgccatgg
     6961 aacgaaacca atgatttctt taggagcccc tgaaattgga aatgaaagag catggggggc
     7021 aagattgaaa caaggaatgg aagtgctttt taaacacacc gatgaagggt ttaatgccat
     7081 gcctcccaga ggaggatgct ttgaatgtac tgataaacag ctgattctag ctatcattgc
     7141 gatggttccc aaacaggctc aaaaaggact attaaacaac ctgttagatc ataaagaata
     7201 caaacagtaa aaaattttta taaaaaagcc taaacttatt caaactgctc ccgatatagt
     7261 taaaaagaga aagagggaga tagaaagtga aaaaacaaat actgttagtt ccggtttgtg
     7321 tcttggctgc ctcttgtgca tccatggata gatcaacggc catagtcgat gatgcgggtt
     7381 atacccatta cactatgagt atggagtccg atagaaaggg ttctaattac tttcctgaga
     7441 aaagacaggc cacaggaaaa aaagtattta tttttgatcc taaagctact gcctgggcag
     7501 cttacgatgc tcagggtaac cgagttaaaa ccggtagtgc ttcaggggga aaagattttt
     7561 gtgaggacgt tggcagaccc tgtcggacgg taacaggcag ctttaaagtt tattccaaga
     7621 aaggcgagga gtgtacctcc agtatctatc caatagaaac tggcggtgga gcaaaaatgc
     7681 cttattgcat gcattttcat ggtggttatt caatacatgc tgcctatgaa gttcccaact
     7741 ataacgcaag tcacggctgt atccgggttt taccaagcgc tgcaaaatgg ttaaatcaaa
     7801 actttattga cgtaggaact tcggtaatcg ttaaacctta ttaataagta aaaattctcc
     7861 actactcccc gctaattgcg gggttttttt attggatgat tttattatct cgtgcagaca
     7921 agaggataat ggtttcgtaa gaagtccagc taaagtttgg gttgggagca aagcggatct
     7981 aacaataatt tgaatcaata atatatagaa tgctctcaat acagggctat ttctttccgg
     8041 gattgctgaa ttattatctt agacctataa tcacaaatca ctagttatgc catttaaaaa
     8101 gtagatttaa taatgattct gtgtattgat gtaggaaact ctcatatata tggtggagta
     8161 tttgatggag atgagattaa actccggttt cgccatactt ccaaggtaag tacttcggat
     8221 gaattgggca tctttcttaa aagtgtttta cgtgaaaaca attgctctcc cgagacaatt
     8281 agaaaaattg ctatttgttc agtagtacct caagtagatt actcattgcg gtcagcctgt
     8341 gtgaagtatt tttctattga cccgttttta ttacaagcag gagttaagac tggattgaat
     8401 atcaaatata gaaatccggt tgaggttgga gctgaccgaa ttgcaaatgc tattgccgcc
     8461 actcatagtt ttcccaatca aaacattatt gttattgatt ttggtacagc aactactttt
     8521 tgtgccattt ctcataaaaa agcttattta ggtggagcta ttttacctgg tcttagatta
     8581 tctgctgatg ctttgtcaaa aaatactgca aagctacctt ctgttgaaat tatcaagacc
     8641 gagagtgttg ttgggcgttc taccatagaa agcattcagt ctggagtcta ctatggtgtc
     8701 cttggtgcat gtaaagaatt aattcaaaga atacatcatg aagcgttcaa tggcgataaa
     8761 atactaattt tggcaacagg aggatttgcg tctttatttg ataagcaagg tctttatgac
     8821 catcttgtgc cagatttggt tctccagggg ataagattag ctgctatgat gaatactgcc
     8881 tgagcagtaa aaggtttttg ttaatatcag atttatttct gtttattcta atggagttta
     8941 aggggtgttt tgtattctgg taagttaatc aaaagagagg atttcgagtc ttataaagca
     9001 ggaaaccctt ttgctcagga tttccatttt tagcgtttta cctcccgctt tttctattag
     9061 aaaaactctg ggctcttcct tctgtttcat taatcaattc aagatcaacc tatgaaccaa
     9121 acaaattcta gaccgctcac ttgcataatc tgtctctttc attttattgc tgcgttggaa
     9181 atgttcttgt ggggctcatt tactctggca aaccctgctc ctcaaaaaat ctgctctttc
     9241 cttgaagcaa aataatcaga tgattcgaga gaacttataa ctgtgagcat acatttggtt
     9301 acaaatcttt cttaaagatc aaataaataa caagtttggg atttgcctct ttaatccata
     9361 aaaaaaatta ccccaaaagt ggtatttcag cttgacgtgt gtagaaatgt gttcctatag
     9421 tgtagaaaag tgggttaaaa taataaaaaa gtggagaatt gtgggagaat aattctcctt
     9481 cacttgggaa ataatatgtt tcgtggaatt aatgccatca ccatagacac taaagggcgc
     9541 ttagctattc cgacgcgtta cagaagtgcc ttgggtgcag aggataaaat ccctctcgtg
     9601 gtgactattg ataccgaaga aacctgctta ctcctttaca cagcagcaca atggcaaatt
     9661 atcgaagaca atttgcaaaa gctaccaagt tttaatgctg cagcaagacg aattcaacgt
     9721 ctgttaatcg ggcacgccac tgatgtggag gttgatgcga atggacgagt acttttaccc
     9781 acagttttaa gaaattatgc aaagttagaa aaagatgtcg tgatgatagg ccagggaaat
     9841 aaatttgagg tttggaataa agaattatgg gaatccaagc gtgagcaatg gctagcagaa
     9901 gaagcgtcca tgacggatgg gttacccgag gaaatgaaaa cgttttcttt ataacggaaa
     9961 gagaaatggc aaagcatcaa tctgttttat tacatgagtc aatcaaaggt ttggctataa
    10021 aagctgacgg catctatttt gatggaactt ttggccgagg aggccacagc cgcgagattt
    10081 taaatcattt aagcgataaa ggaagactgt ttgcaataga taaggacctt gatgcggtgc
    10141 aatatgccaa ggattatttt ggtttggata aacgatttca aatttttcac ggttcattcg
    10201 cgcaaatcaa ggaatttgcg tcacaagcgg gagtaattgg ggccgttgat ggtattttgc
    10261 ttgatctggg agtgtcttca cctcagttgg acaatccaga aagaggtttt agttttatgc
    10321 tgcaaggccc tcttgatatg cgaatggatt taactcaatc aattaatgct gcgaattttg
    10381 tcaatgaagc agaagtcaac gagctggctc atgtattcag ggcttatggt gaggaacgat
    10441 ttgcaggcag gattgcaaaa gctattgttg atgcaagaaa actaaaacca ataacaacta
    10501 ctttggagtt agcagaaatt gttaaagaag caaaccccaa atgggaaaaa cacaaacatc
    10561 ctgcaacccg ggtatttcaa gcgattcgaa tacatgtcaa tcaggaatta accgatttga
    10621 gtaattgtct agagcaatgt ctggacgtat tggggccaag aggacgcttg gccgttatca
    10681 gtttccactc tttggaagac cgaatagtca aacaatttat gagagataaa gagcaaggta
    10741 acaggccgcc tgttgaagtc ccaatcaaat acgaagaact aaagaccaat tttaaaagag
    10801 taggtaaagc agtgaaacca cagagtagcg aaataaagga aaatgttaga tctcgaagtg
    10861 cagtacttag gataggggag aaattagcat gaacgctgca gcgaaagtca ttaatcaagg
    10921 taatttattt aatggtcaat tggcagatat gcaaatgtca aaatcactct atatgttgat
    10981 agtattgtta gctgccgtac tagtcagtgc atttgctgtc atatatagca ctaattccta
    11041 cagattaact ctcagtcaag tgcaacaaga ggaacagcaa acccactttt tacaattgca
    11101 atggggacaa ttattattgg aacaggccag tttggcaact ccagcccggg ttgaggaatt
    11161 agccagagaa aaattgggaa tgactttacc aacttctgca aatacgtact tactgcatcc
    11221 tcaataagtg agtcgtttta gaggagttaa agtgtttcca tattttgagt tataggtaag
    11281 agctgagtac ctggtgaagg ataatctatg tccaagagca tcaaattaaa gattgaataa
    11341 tgaaatttca aaggatttga tagagcaatt aggcgcaatg cggaggaaat cacctcgaaa
    11401 aaattgaaag cttcatgtac acttacacgt cattcatctt ctttccagga cgtcatggaa
    11461 gactaagtgt aaaatagcct gataaattcc aggcaggtat acgtgcctta tgtttaactc
    11521 taaggtaatt ttgagaaaga ggtttcaagt atattttcac taatttattg ttgcgtgcat
    11581 agctaatact atttatgagt agtcatatat gaaaaaaaca caccattttg ccaggctggt
    11641 tactgtcgct gtttttttct ctttgatttt ggctgttttg atatggagaa tggttgatct
    11701 cactgttctg cataggcaat ttttgcaggg ccagggtgat gcgcgcagca tccgagttat
    11761 agatattcct gcctatcgag gtatgatcac cgatagaaat ggtactccgc ttgctgtgag
    11821 cacacctgtt gaatcggttt gggtcaatcc aaaggaattt tctccggatg aagaacaatt
    11881 tatgcagtta gctaaatacc tgaatttggc acctaaagaa ttaagtagaa aaattgtccg
    11941 ggccgaaaat cgggagtttg tgtatttaca aagacagtta cctccacccc tttcgaaaaa
    12001 gatcgaagct ttaaaaatcc caggtgttaa ttttcaaaaa gaattcaaac gttattatcc
    12061 agattcagat agtatttccc cattaattgg atttaccaat gtcgatgatc aaggattgga
    12121 ggggattgag ctggcctatc aagattggct tatgggagtc gttggtaaaa aaagagtaat
    12181 caaagatcga ttaggacgag ttgtcgaaga tctgggagtt ttgaaggaac cacgcccggg
    12241 acatgagtta actttgagta tagatagacg actacaatat ttagcttata gcgaattagg
    12301 taaaacagtt gaagagtttt ctgctaaatc tgggtctgta gttgtaatcg atgctgaaaa
    12361 tggtgagatc ctggctgtgg ctaatgttcc atcttataat cctaacgcac gtgggcatta
    12421 tgataaggaa acctaccgca acagagcctt caccgatact ttcgagcctg ggtctgtcat
    12481 taaatcattc agtatagcca gtgctttgga aacaggctta tttactccgg ataccattat
    12541 tgatactaat ccaggatgga tgactgtcca tggccgaaca gtaagagata tccataatta
    12601 tggtgtttta gatgttaccg gaatattaca acattccagt aatgtgggtg ttacaaaaat
    12661 ggtattatcc actccagctg aacaattaat cgggtttttg caaaaatgtg gttttgggca
    12721 acgctctgaa ttggcatacc cgggtgaaag tgaaggaggc atagtaaaag ccaaggatgc
    12781 aaacccattc gttttagcca cattaggttt cggttatggc ttgtctgtta ctgcactgca
    12841 attggctaaa gccaatttaa tttttgcaaa taaaggacga ttaatacctg tgactttgat
    12901 tcacaatgat acccccagta atggcattca agtgatgcaa cctaaaaccg ctgaaaatgt
    12961 attattaatg atggaagcgg taatgaataa gggtacaggt aagggagcaa gggttcctgg
    13021 ttatagggta gctggaaaaa ctggaacagc acgagttgct ggaaagaatg gatataaaga
    13081 caggaaatac acagccagtt ttattgggat agcaccagtt tccaaaccaa agtttgtagt
    13141 cgctgttatt attcatgaac cttcacgaaa gggttattat gctgcctctg tggcagggcc
    13201 cttatttgct aaagttatgt caggtgcttt acgcttattc aatattccta ctgatgagcc
    13261 agttagttaa agaagaatag gagccaattt tgtgaaatta acccaattat tagaaccgtg
    13321 gattaaaagg gacattattg atggcactgt gagtgacata aaaaatgaca gccgcttggt
    13381 gaaagaaggg gatttatttg tcgcctatcc aggggcagct tccgatggtc gattatatat
    13441 agaaaaggcc gtggcatcag gtgctgtcgc tgtggtttat gagcccaata atttacctca
    13501 gggaattact ttgccaacag caattccctg tatagccatt ccccaattag ctgaaaaatt
    13561 aggggaaata gcgaaacggt tttttaataa cccatcccaa tttcttggtt taactggggt
    13621 aactggcaca aatggcaaga cgactattgc ttatcagttg gcacaggcac atgatttact
    13681 cgggcagcac tcggcttaca tcgggactat aggacaaggc aaggtggatg ctttaaaacc
    13741 tttggaaaat actacgccag atgctctttg tttgcagagg ttgatgcacc agtacaaaga
    13801 agagggcatt aaacaggttt gtatggaggt atcttctcac gcgttgagcc agcatagagt
    13861 cgactctctc gaatttagtc aggctatctt taccaattta acgctcgatc atttagatta
    13921 tcaccaaact atgcaagctt atggggctgc gaagtcgagg ttgtttgaac gagcagagtt
    13981 gcaatgggct attattaatc aggatgacga ctttcaagat ctcatgagca atgtgctaaa
    14041 gactcatgta aaaaaagtga cttacggtat gcatcaaaat tgcgatgtga aagcgctcca
    14101 atggaatatg gatatcaccg gaacagaaat tgaagtcaaa tctccttggg ggcaccagct
    14161 tctaaagatt aaagcactgg gtaagtttaa tatttataat agcctggctg tttatagcag
    14221 tttgcttgcc agcgggcata atccagaaaa agtagtgcaa gttatggccg aattaaaagc
    14281 ggctcctggc cgaatggaaa ttgtcgctca atccccatat gttcttgttg attatgctca
    14341 tactcccgat gcattggaaa atgttcttat caccttaaaa caaataaaaa aagggagatt
    14401 gtgggttgta tttggttgtg gtggggacag agataaaacc aaacggccca ttatgggaaa
    14461 ggcagccagc atgtacgctg ataatattgt cattaccagc gataatccga gaagtgaaga
    14521 tccggaaatt attctaaatg aaattgcttg tggcattcct gcctctgcgc ctgtctctaa
    14581 actggtgaat agagaagagg caattgctta tgctttaaga aaggctgagc ataacgatat
    14641 catattaata gcaggaaaag gacatgaatc gtatcagcag attgggaata caaaacatgt
    14701 tttttctgat caggaagttg taagacgttt gatgaataat taaaatttcg ggacaggggt
    14761 taattattag aaacagacat caacacttta acctaataat ggatactttt ttattgagtc
    14821 agaatcaaaa taagttgtga aatcatggcg gtgatgatga ttcttgcgaa gagtcaagaa
    14881 tgctgaggat agatttccag cgtgttttaa ttctagtgtt tcatgaatcg ggttatatat
    14941 tgataaagcc aactctctcc agtcgctgtt ttcatttaac ctataaggtt tttcaggggt
    15001 tgcaaattgt gggtatggaa gaccaagcat ttgatgtaat ttacgactga cgattgcgac
    15061 tgcagcaaat ttagcttcga ggctgtgtcc tgcaatatgt ggtgtgcaaa gagttgcctt
    15121 ggagacaatc cggctatcta tatgaggttc gttattgtag acgtcggtgc aataaagtat
    15181 ggcagagcca agatgcaaaa gggcttcttc attaacgata cctcctcgag aagcattgat
    15241 gataatgcag ccaggtttta attccttgag aaaatcccga ttaattaagt taaggcttgg
    15301 gtagggcgca tcgctatgta attcagcatg aacacataag aggtcacatt caaaaagatc
    15361 ttcaagagag cagctttgaa aagaagtatc tctggttgct ttgggtgggt cataaaggca
    15421 taactgaaat tcggcagcat ttagtcgttc ataaacttta gtacctactt gccctaaacc
    15481 aataattcct gctgttttac cttgaataag ttgctgttta tctaaatagg caaggcaagc
    15541 aacaacatag tcagcaaccg atatggcatt gcatcctttg gcatcaataa tagatatgtt
    15601 ttgtgattct aaaaaaggaa aatcaatatg atcggttcca ctggttgctg tagcaacaac
    15661 ctttatttga tggtttttta gtaaatcgcc attgattttt agagttgaac gacaaagaag
    15721 aacgtcctta tgatgaagca attctggaat ttcatcggcc ttatgataca aggttacggt
    15781 aaatggtggt ggaaaagcac tatccaatcc tggcaataaa gcatctgcta aaatattcat
    15841 aaattaaaaa tcgcgcttaa aatatataat gaaccataga tgaggggagt aagaatatat
    15901 cccaatacat tagtaaaaat taatacaatc aatattaaaa aaccaaatgg ttctaatttt
    15961 gcataagcga tagcttgtct tgctggcaat agatttatta caactttgct gccatcaagt
    16021 ggcggaatgg ggattaagtt taaaaaggca agaatcaaat tgatcatcat tcccgctctg
    16081 gctgtaacaa gcaggaataa agcagccatt gagctttgag gatttaagaa agttgcaatt
    16141 ttaaaacatc cagcccatag gaatgccatt atcagatttg ctaatggtcc cgcaagcgtt
    16201 accaatatca tatctcggcg tggttttcgc agttggctct ggttaaccgg aaccggtttt
    16261 gcgtaaccaa ttacgaaatt gaaatttgtt atcacgccaa ttaaaagagg taatagaaca
    16321 gtgcctattg gatcaatgtg tttgataggg tttaatgtta atcgaccgaa cattttagct
    16381 gtgttgtcac cgcagcggta ggcaacgtaa ccatgagcta actcatgaaa agtaatagct
    16441 aataatactg gtaaagccca aatacaaatt tgttggatta acgtgaattc tggcattaat
    16501 attctgatgt atttaataag gatcctgatt ttaacgaatt ctactcgcta ataacaaggg
    16561 ttgatgaaaa attgtgtggc cgctcgcatt tgttgatatt tttaccatcc ttgccgaaaa
    16621 atcttgattt tctactcaag cgtgagaaat ctcgacagga cctaattttt attaataaca
    16681 tggggtcttg gctaaatcga aagcatctta tatgattcca tttgatatga ggatttataa
    16741 ggcttggttt cgatgacgca atttgagaaa act
//
LOCUS       DASLOE010000053        16636 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.053, whole genome shotgun sequence.
ACCESSION   DASLOE010000053 DASLOE010000000
VERSION     DASLOE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16636
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.053"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            530..1429
                     /locus_tag="U6309_001393"
     CDS             530..1429
                     /locus_tag="U6309_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEN5504842.1"
                     /translation="MEESELRFRSAFNSAAIGMAIVSLEGMWLKVNQSLCQIVGYSEE
                     ELLETNFQSITYPEDLELDLGYVRQLLEGDIRFYHMEKRYIHKNGSIIWILLSVSLIR
                     DPENKPLYFISQIQNIDAQKRAEQELQHIAYHDTLTGLGNRKLLELSFDQALAHAKRH
                     QTQIAIMFMDLDYFKNVNDKLGHDIGDLLLVEIGKRLQTILRSTDLIVRHGGDEFIIA
                     LTELSNVNQVIEIANKILIAISKPITIKRYGISITGSMGISIYPYDGDDSDTLIRKAD
                     EALYRVKIGGKNNFQLFNTALHK"
     gene            complement(1681..2067)
                     /locus_tag="U6309_001394"
     CDS             complement(1681..2067)
                     /locus_tag="U6309_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504843.1"
                     /translation="MDIVKAQKDMKVKVNVLRIPANEREANIVAVYSILINKDLMGDM
                     DHIPNVIWQIKSIIENINLDDDDIARSICLIKEKIENSNENYTNKNIMDFLNAFSKKS
                     DLTFRQIRQELAKSNSEMKKILDTYD"
     gene            complement(2078..2356)
                     /locus_tag="U6309_001395"
     CDS             complement(2078..2356)
                     /locus_tag="U6309_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504844.1"
                     /translation="MPLSVSDCQSNFPVMNNGHHLLHQKQDGIEKSKITQTAAQAANN
                     TNSAKVTIESEYDVDSEKKVTKVGEVRRFFIPGLIQKDYFNINLIFIT"
     gene            2598..5459
                     /locus_tag="U6309_001396"
     CDS             2598..5459
                     /locus_tag="U6309_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEN5504845.1"
                     /translation="MNTHNAVLTCFWGLGYSDVKGVCHGISIRWLEACMSGEEHVFHE
                     RLKRIEQIVSSGQDIVKLMDVVKEKKGKNLSAEDQDLLDIQAFFDSMDLYHTSYRHTE
                     LFGLSSNSNYTNIAFHSDFAASDKISALGGLTQVYSQALIPNEVELKHYLDELGFIMQ
                     AGSGSRKDVLGVLLSSFDHTLALTYKPGNGWGFMDINQYPSKVFKTEETGLLAKMIIK
                     GLNSVPSSNYIALDASIYTTTRNPQVYSLSTQLGQFKSSHVITPEIASREQNGMGLAW
                     IAARIGDIALISELAKHQIDLNKAEENGFTLAHLAVQQGDVALISELARHGVDFNKPA
                     KHGVTTAHFSAQQGDVAVIAELAKYGVDFNKADESGVTPVHLVARNGHIAAIAELAKH
                     GVDFNKASRSGDTPAYLAAQNGHAAVITELAKNNVNVHEPNEHGSTLAHIAAQKGHVN
                     VIAELAKHQVDFNKKGINGSTPAHLAARNGHVAVIAELAKHQADLNKADNDGFTPAHI
                     AALTGQPLILDELIKLGADLSPSLISAIHWKNKTYLKQDAVSRMNSFLQFHKGKQEVL
                     LSPGELAVIMGDENQIQRYLDKVIEPHFRSANRADCAEKKNTRGEPLPREVIACLNQL
                     IDLKRQKEMLELQFEETDALLEAIKENEERVIGRKYYIDFIDEHKESYTNVMWHISNP
                     VKEELFQNEAQLRHSGLSTTVLYGMSWATSFLSSCYRFLTPKLQQEYLDSFMPQTLDS
                     QSKALFKKLLQEHKDSLKKEMVRSEQFITNINKKLFPESEEMLPLFNTLSSKNLQLLP
                     IYEFRIMKSRLAEDSDILKEPIDAQSLMHKKSSMSILRIPATEREENIYAVFQMLECP
                     LMCNEDGSIPKILKEIKAIIKEIDPQEEQSISDALHLIRNVIKDDARQERPLVAEVLK
                     IFDATGSLNFRQIRNSLEHLPGLNEFSLSLRRPLSGHST"
     gene            complement(5492..5980)
                     /locus_tag="U6309_001397"
     CDS             complement(5492..5980)
                     /locus_tag="U6309_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEN5504846.1"
                     /translation="MKIRAIFFMIAIFFPVTLFASNTTCPLSNGINVLTKKRILNICK
                     NGTVVKTFKVALGYKGVGKKKAGDNKTPIGLYGLAHPRKSNQFKVFIPILYPTKKQLA
                     AGYTGRDVGIHGPTQSPGVFSWLNNLPGSTRGCVAVGKNNYIEYVANWVKANPGAKVL
                     II"
     gene            6546..8423
                     /locus_tag="U6309_001398"
     CDS             6546..8423
                     /locus_tag="U6309_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN5504847.1"
                     /translation="MMNESSTEKKNELSLSFAALGVVFGDIGTSPLYAFGQVIKYFPI
                     NDHNIYGILSLIFWSLIIIVSIKYLVIVFRADNDGEGGIIALAGLIRQKIKKPGGWLL
                     FITLVGIGLIIGDGILTPAISILSAVEGLESLSPNLAKYVLPVTLIILFFLFKMQSIG
                     TGKIGVYFAPVMLIWFITIGVLGFLQIIQNPKVLMAINPYYAIYFFMIHKYFALFILG
                     GVFLVMTGGEALFADLGHFGKKAIRTGWFAVALPALLLCYFGQGAFVLMHTENIKYPF
                     FNLSPDWFLPVMIILATIATIIASQAIISAAFSILKQASLLNLIPRLKIIYTSKFEKG
                     EVYLPLINFILALGTCSLVVIFKSSSNLADAYGIAVNLDMLITTVLVGIIAYYCWSWH
                     AFKVMIFPLILVIELAFFAGNIPKLLTGGWIPILIAFLGFVVMYTWHCGFEKLRELHH
                     RDALMDAFIIDELNQNKISRQSGMGLYIIDPYDCEGESLLHHLRLNRIFFENMIFVSI
                     KIENKPYIPIEDKFELIKKAEGFYLIFIHYGFTENINLPNELDEMFKRVYLPFEIIKN
                     KLIYSIEIVFVEMTRERQKHMYMWQKHLFSLMIRNAVPDIQFYRLPYNKTIAIGTYYQ
                     L"
     gene            8474..8935
                     /locus_tag="U6309_001399"
     CDS             8474..8935
                     /locus_tag="U6309_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEN5504848.1"
                     /translation="MNKADLHNCVLFKGIDEKYIDDFLENCEEVELPEGEFLFHQNEI
                     GDSMYIVEQGELQIILDQNSTDSNPQEEQVIGVLKSGALFGELCVFGQLKRAASIRAL
                     VDCRLLKIEGEDFRIRIYSKDLDALLICYNIAKLLSQRFITLLSLRPSLNM"
     gene            complement(9276..9794)
                     /locus_tag="U6309_001400"
     CDS             complement(9276..9794)
                     /locus_tag="U6309_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5504849.1"
                     /translation="MDFLEQHYHLSQIDATLVNGFSTLGYIVGAPFFGVLSHKVKNVI
                     PIMFYAALIESILLAFNILYLHDLHLKMIFYAIVGFFTGAVTIGFSLVKDYVKPTMIG
                     TWIGILNMGQICVGMILSLLFGELLDLFHDNYIWAAIPVILCSFISVYASYWLWKKSQ
                     SPELPARAPYKH"
     gene            complement(9886..10797)
                     /locus_tag="U6309_001401"
     CDS             complement(9886..10797)
                     /locus_tag="U6309_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEN5504850.1"
                     /translation="MTIQKGLHERRALKISIAVTFLLAVVGILFGLLSGSLAIVFDGM
                     FNMVDTVISILAFFVARLLTSKGNRRFQYGYWHIEPMVLVLNGSILILLCTYALVNAI
                     GSLMSGGHELNFDYAFVFALLVFFLSTGMYFYLVKTNRKIKSEFLRLDIQSWLMSALI
                     STSLLLAFGIAALLHGGAYGYFTPYIDPLILAILTAFLIFVPMAAVRDAMRDIFRMAP
                     VGLDERIREFLDELIKQHDFKTYTSYVAKIGRAQFIEIHIVVPMDYPISSIETLDKIR
                     NEIALAIGEDTPQRWLTIAFTANENWI"
     gene            complement(10954..12360)
                     /gene="dbpA"
                     /locus_tag="U6309_001402"
     CDS             complement(10954..12360)
                     /gene="dbpA"
                     /locus_tag="U6309_001402"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEN5504851.1"
                     /translation="MIQSEHNDQHLSFSQFPLRQELIKSLASSNYENMTPIQMQSLPI
                     ILRNEDIIAQAKTGSGKTAAFALCLLNNLKISFFAVQALVLCPTRELAEQVSQAIRRL
                     ACLMPNVKIINLSGGIPMQPQLDSLRHGAHIIVGTPGRILKHLKNASLDLSQVKALVL
                     DEADRMLDMGFFDDIKNIISVCPKQRQTLLFSATYPEEIKRLSKQFMKDPKEVHVVTP
                     PEEIDIEQHFYEVSRHTQKFPLLKSLLLHYRPVSVLIFCNTKQETVEVTDQLINEGFS
                     AIALNGDMEQIERDLAVLRFANQSCSILVATDVAARGLDIKELSAVINFDLAFDHDVH
                     IHRIGRTGRAGSKGIALSIVTPADAQRICAIEDNYPHPIHWRNINELENHNTARLVPE
                     MVTLCIASGKKDKIRPGDILGALTKDAGLASNTIGKINITAMYSYVAIHHSQADKAYQ
                     YLQSGKLKGRKVNVRKIN"
     gene            complement(12600..12809)
                     /locus_tag="U6309_001403"
     CDS             complement(12600..12809)
                     /locus_tag="U6309_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN5504852.1"
                     /translation="MSKTVNGVVKWFNEIKGFGFIEQEGGPDVFAHFKEISSSGFKTL
                     TEGQRVQFIVTQGAKGLQAQNITAL"
     gene            13072..14086
                     /locus_tag="U6309_001404"
                     /pseudo
     CDS             13072..14086
                     /locus_tag="U6309_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947830.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14547..14843)
                     /locus_tag="U6309_001405"
     CDS             complement(14547..14843)
                     /locus_tag="U6309_001405"
                     /inference="COORDINATES: protein motif:HMM:NF016876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN5504853.1"
                     /translation="MSNKTYRLYPKAVEDLESIYLYSTNEFGIKRTEDYILAIDLSFQ
                     RLADDPLIARKCDYIRPELRAFNVGSHIIFFKTTDYGISVIRVLHQSMDFNRHL"
     gene            complement(14833..15087)
                     /locus_tag="U6309_001406"
     CDS             complement(14833..15087)
                     /locus_tag="U6309_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEN5504854.1"
                     /translation="MTRTMTVDLGNELRNYVESLVRSGDYRSNSEVLRESLRLLREKQ
                     AESKLEQLRHLIDEGAASGKPETWDAHDFLKKMKKKSHVK"
     gene            complement(15435..16001)
                     /locus_tag="U6309_001407"
     CDS             complement(15435..16001)
                     /locus_tag="U6309_001407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504855.1"
                     /translation="MNLRHPLILRKTPAIITDNMEHNSQYIYGMDPFYFHSYLMSIAR
                     KDYPYLAQCVTEKFCKSAFKLNQIENALGYKYGNDELRALGTNERDIMSTLERLAKAY
                     NTNIHVITSEHILQYQQAKKEGKAFNEVTNSKKLEIRAGTIKSNGFTFKDTVGVVLFK
                     KPYQGWQEYKKHKNSHSIMEASEKKRGI"
     gene            complement(16106..>16636)
                     /locus_tag="U6309_001408"
     CDS             complement(16106..>16636)
                     /locus_tag="U6309_001408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504856.1"
                     /translation="YLFYYQSRLPKDAHRHAPVLANVSVFLSNPSLITQALAVLEQEV
                     KFLWDFENSTRTADKQLKLNQSIQFCRFMNLLWPVPNEELISNKTNNLLSKVLLYREQ
                     KHGFNFDENLSQRPVPTFVGFVDKKEADKVLEKNQLWSEENKLSPLFFHGKETHRLQL
                     NLIMIAVELGLLDIGG"
BASE COUNT         5238 a         2859 c         3146 g         5393 t
ORIGIN      
        1 tatgtacatt aattatttat attggtttta ataaaacaac agatcaagaa atgctctctg
       61 tatggtttat ggttggagta acagtgtttg ctctccatgg aggtttattt ttatttaatt
      121 attatcgtcc cttccatcca aatacctttt aaaatggtta ataggcgtca ctgcgattta
      181 tggggctcta tggggaatag caggttcggt tcttattcct cataatgatc tgttaaatca
      241 aatgattgtc ataattataa ttattggaat cgcatcaggt ggattacata ttcttcagcc
      301 cagtttttta gcaagtctat tattttttac attaaccctt attcctctat gtgtctggtt
      361 tttccagcaa aatacactta actattggtt ccttggaact gcattgctga tttacttttg
      421 ttttttatca attatatcct ggatgggata tggacttctt aataagaatt ttaaattacg
      481 ttatgagaat ttggatttaa ttaataaatt gtatgcaatt aacgctaatt tggaggaaag
      541 tgagttacgt ttccgttccg catttaattc tgctgctatt ggtatggcta tagtttcttt
      601 ggaggggatg tggttaaagg ttaatcaatc actttgtcag attgtaggat actcagaaga
      661 agaattattg gaaactaatt ttcaatcgat cacttatcca gaggatttag agctcgattt
      721 gggctatgta agacaattat tagaggggga tataagattc tatcatatgg aaaaacgcta
      781 tattcacaaa aatggtagta tcatttggat tttacttagt gtttctttaa tccgagatcc
      841 cgagaataaa ccactctatt ttatctcgca aattcaaaac attgatgccc aaaaacgagc
      901 agagcaagaa cttcaacaca tcgcatatca tgacacttta actggtttgg gaaatagaaa
      961 gctattagag ttatcatttg accaggcatt agctcatgcg aaacgtcacc aaacgcaaat
     1021 tgctattatg tttatggatt tggattattt taaaaatgtg aatgacaaat tgggtcatga
     1081 tattggggat cttttattag ttgagattgg aaaaagatta cagacgattt taaggtctac
     1141 tgatctaatt gtcaggcatg gtggtgatga attcatcatc gcgcttactg aattatccaa
     1201 tgtaaatcaa gttatagaaa tagcaaataa aattctaata gctataagca agcctataac
     1261 aatcaaacgt tatggtattt caattacagg cagtatgggt ataagtatct atccatatga
     1321 cggcgatgat tcagatacat taattagaaa agctgatgaa gcattatata gggtgaaaat
     1381 agggggaaaa aataattttc aattgttcaa taccgcacta cataagtaaa ataatttttt
     1441 aatcaacaag cttctttcca gcccctttgt tcttaatttg tcagattatt accctgtttc
     1501 gtctctaaat atcaacattg ccattttctt ggttatctgt ctgacgagag atgccatatc
     1561 gatggcaaac agagcctcta ttttttagta tcaaggtaaa aattagccta tgcttttttg
     1621 cttattttag acgatggata atacgaatga attgaaatga tgtctatgaa tcacatcatc
     1681 tcaatcatag gtatccagaa tttttttcat ttcagaattg gattttgcta attcctgtcg
     1741 tatttgtctg aaagtcaaat ctgatttttt actaaatgca tttagaaaat ccatgatatt
     1801 tttattggta taattttcat tggaattttc tatcttttct ttaattagac aaattgatct
     1861 tgcaatatca tcatcatcca gattgatatt ttcaataatt gattttattt gccaaatgac
     1921 atttggaata tgatccatat ctcccatcag gtccttgtta attaatatcg aataaacagc
     1981 tactatgtta gcttctcttt cattagctgg aattcttaaa acatttactt ttaccttcat
     2041 gtctttctgt gcttttacta tgtccacttc aatcctctca ggttataaaa atcaaattga
     2101 tattaaaata atccttttga attaatccgg gtataaaaaa tcgtcttact tctccgactt
     2161 tagttacttt cttttcacta tcgacatcgt attcagattc aatggttact tttgcagagt
     2221 ttgtattatt agctgcttga gcagcagttt gtgttatttt ggatttctca atcccgtctt
     2281 gcttctggtg aagtaaatgg tgcccgttgt tcatcaccgg gaaattcgat tgacagtctg
     2341 atacagacaa aggcatatga attccaaact aagtgtattt atatttaaat aaaagtatag
     2401 aacaaatacc aagagttttt attaccaaaa aattgatatc tgggttaagt caaacacatt
     2461 cataaggacc agctttatta gatcaaactt tctatttgat ctgatgagat cgggggggct
     2521 tgtactctac atcatggttc gaagcataat acttgtgtct ataattataa ctacaactaa
     2581 taatctgatt tcctaaaatg aatacacata atgcagtatt aacttgcttt tggggattag
     2641 ggtattccga tgttaagggc gtatgccatg ggatttccat tcgctggctg gaggcctgca
     2701 tgtcaggtga agagcatgtg tttcatgaac ggcttaaacg cattgagcag attgtttctt
     2761 caggtcaaga tatagtgaag cttatggatg tggttaaaga aaaaaaaggc aaaaacctat
     2821 cagccgaaga tcaggactta ttagatatac aggctttttt tgacagtatg gatttgtatc
     2881 atacgtctta tcgccataca gaactatttg gtctttcatc taatagtaat tatacgaata
     2941 ttgcatttca ttctgacttt gcggcatcag ataaaatcag cgcgttggga ggtttaactc
     3001 aggtttattc tcaagcactt attcctaatg aggtagagtt aaagcattat ttggatgaat
     3061 tgggtttcat catgcaagct ggttctggct caagaaaaga cgttttagga gtgcttttat
     3121 cctcattcga tcatactctg gcattgactt ataaacctgg taatgggtgg ggttttatgg
     3181 atattaatca atacccatct aaggttttta aaaccgaaga gacaggatta cttgctaaaa
     3241 tgataataaa gggtttgaat tcagtcccgt cttccaatta tatcgctttg gatgcatcca
     3301 tttacaccac aacaagaaac ccacaagtgt attcattaag cacacaattg ggtcaattta
     3361 aatccagtca tgtaatcacc ccggaaatag catcccggga acaaaatggg atgggtttag
     3421 cctggattgc agcccgaata ggagatattg cacttatctc agagcttgcg aagcatcaaa
     3481 ttgatttaaa taaggcagag gaaaatggct ttacattggc tcatttagct gtccaacagg
     3541 gtgatgttgc actcatatcg gagcttgcaa gacatggagt tgatttcaat aaaccggcta
     3601 agcatggtgt tacaacagcc catttttcag cccagcaggg tgatgttgct gtgattgctg
     3661 agcttgcaaa gtatggggtt gatttcaata aagcagatga aagtggagtt acacctgtac
     3721 atttagtggc tcgaaatgga catattgctg ctattgctga acttgcaaag catggggttg
     3781 atttcaataa agcaagtaga agtggggata caccagcgta tttagcagct caaaatggtc
     3841 atgctgccgt tattactgag cttgctaaaa ataatgttaa tgtccatgaa ccaaatgaac
     3901 atggttctac actagcccat atagcagccc aaaagggcca tgttaatgtg attgctgagc
     3961 ttgcgaaaca ccaagttgat ttcaataaaa aaggcatcaa tggttctaca ccggctcatt
     4021 tggcagccag aaacgggcat gttgcagtta ttgctgagct tgcaaagcat caggctgatc
     4081 tcaataaagc agataatgat ggctttaccc cggcccatat agctgcatta acaggtcaac
     4141 ctttgattct tgatgaatta ataaaattag gcgctgatct aagtccatct ttaatttcag
     4201 ccattcattg gaaaaacaaa acctacttga aacaggatgc tgtttctcga atgaattcat
     4261 ttctgcagtt tcataaagga aaacaagagg tgctactctc gcctggagaa ctggcagtta
     4321 ttatggggga tgaaaatcag attcaacgtt atctggataa agtgattgaa cctcatttta
     4381 gatctgccaa cagggcggat tgtgcggaaa aaaagaatac gcggggggag ccattgccga
     4441 gggaagtgat tgcatgtctt aaccagctca ttgatttaaa acgccaaaag gaaatgcttg
     4501 agttacaatt tgaagaaaca gacgcgcttt tggaagcaat caaagaaaat gaggaacgcg
     4561 tcataggcag aaagtattat attgatttta ttgatgagca caaagagtcc tatactaatg
     4621 tcatgtggca tatatccaat cctgtaaaag aagagctgtt tcagaatgaa gcacagcttc
     4681 gtcactcagg actttcaact acggtacttt atgggatgag ttgggcgacg tcattccttt
     4741 cctcctgtta ccgttttttg acccccaagc tacaacaaga atatcttgac tctttcatgc
     4801 cccagacttt ggatagtcaa agtaaagcct tgtttaaaaa gctcttgcaa gagcacaagg
     4861 atagcctaaa aaaagagatg gtcaggtccg agcaattcat tactaatatc aataaaaaat
     4921 tattccctga gtctgaagag atgctccctc tgttcaatac gctgtcgtca aaaaaccttc
     4981 aactcttgcc tatatacgag tttcgtataa tgaaaagcag acttgcggaa gacagtgata
     5041 tactaaaaga gcctatagat gcacagagct tgatgcataa aaaatcatcg atgagcattc
     5101 taagaattcc agcgactgag cgcgaagaaa atatttacgc ggtatttcaa atgctagagt
     5161 gccctctaat gtgtaatgaa gacggctcaa ttcccaagat tttaaaagaa atcaaagcga
     5221 ttataaaaga aatagaccca caagaagagc aatcaattag tgatgcgctt cacctgattc
     5281 gcaatgtgat aaaagatgac gcccgacaag agaggccttt agtcgctgaa gttctaaaaa
     5341 tatttgatgc tacgggtagc cttaatttta gacaaatccg caattcgtta gagcacttac
     5401 ctggtttaaa tgaattttct ttatcattaa gacgaccact atcagggcat tctacataaa
     5461 ataaataggc ggtatcaaat ttcgtctcaa tttaaataat taaaacctta gctccaggat
     5521 tggcttttac ccaatttgcc acatactcga tgtagttgtt tttgccaaca gcaacacatc
     5581 cacgagttga gccgggtaag ttgtttaacc aactaaacac gcccggcgac tgggttggtc
     5641 catggatccc gacatctcta ccagtatatc ctgcggctaa ttgcttttta gtcggataga
     5701 gaattggaat aaagacttta aattgattgg atttccttgg atgggctaac ccatataaac
     5761 caattggagt tttgttatcc ccagcctttt ttttaccaac gcctttgtac cccagtgcta
     5821 ctttaaaggt tttaacgacg gtgccgtttt tacaaatatt taaaattcgc tttttagtga
     5881 gtacattgat tccatttgat aaaggacaag tcgtattact ggcgaataaa gtaactggaa
     5941 aaaaaatagc gatcatgaaa aaaattgctc ttattttcat ataaataggc taatattgtt
     6001 gaatttgggc aataattatc ccacaatatt gtttttattc atagagctgg caggctaaat
     6061 cttatgtaaa tatacgtagc ttgaaagtct gtttaggaaa aaatcaattt ctaaggcatg
     6121 ctctgaagaa ctctccctca gaaatcttgg ttcgaagcca agtattttag ccatgacaaa
     6181 ctttaaaata aagatttgat gaggcgtcaa tatttgataa tacagcctaa taccatctga
     6241 agctgtccga cttatataaa aattattgat acagaaaact ggtttatacc gatgagatgg
     6301 cgattaatga taactaagaa gatctggaac aatttaaaaa aaaactgaga aaatatatca
     6361 aaaaacttga aagatgtatg ggcctattgc ttatgcaatg aggaaggcat tttacagttg
     6421 gcgatagaaa taaataattc accaagcaca ggatacttgg agataatgtt tttttaatat
     6481 cagatagtac tatactcaaa atagtaattc ataaaggatc ttaattatat tgggagtaac
     6541 cccggatgat gaatgagtct tcaacagaga agaagaacga gctcagctta tcatttgcgg
     6601 ctctgggggt tgtttttggt gatataggaa caagtcctct ttatgcattt ggccaagtaa
     6661 taaagtattt tccaatcaat gaccataata tttatggtat tttatcgctt attttttggt
     6721 cgctgatcat tatagtcagt attaagtatc tggtaatcgt gtttcgtgct gataatgatg
     6781 gcgaaggcgg cattatagct ttagcagggt tgataagaca gaaaataaaa aaaccaggag
     6841 gctggctttt atttattact cttgttggca tagggcttat cattggtgat ggaatattaa
     6901 ctccagcgat atccatatta agtgctgttg aaggtctgga atcgttgtct cccaatttgg
     6961 ccaaatatgt tcttccagtg acattaatta ttttattttt cctctttaag atgcagagca
     7021 taggtactgg gaaaattggt gtttatttcg ctcctgttat gcttatttgg tttattacca
     7081 taggcgtttt gggtttttta caaattatac agaatcccaa agtactgatg gcaataaatc
     7141 cttattatgc gatttatttt tttatgatac ataaatactt tgctcttttt atattgggag
     7201 gggtgttttt agtaatgact ggtggtgagg ctttatttgc tgatttaggg cattttggca
     7261 aaaaggccat tcgaacaggg tggtttgctg tggcgcttcc tgcattactt ttatgctatt
     7321 ttggccaggg agcgtttgta ttgatgcata cagagaatat caaatatccc ttttttaatt
     7381 tatcgccaga ttggtttctc cctgtgatga ttattttggc taccattgca accattattg
     7441 catcacaggc aattatttca gcggcatttt cgattttaaa gcaggcgtca ctgcttaatc
     7501 tgattccacg tcttaagatt atctatacgt ccaaatttga gaaaggcgaa gtatacttgc
     7561 cacttatcaa ttttattttg gctttaggta catgttcact tgttgtgatt tttaaatcat
     7621 catctaatct tgccgatgct tacggcatcg ctgttaattt agatatgctt atcacaacag
     7681 ttttagttgg gatcattgca tattattgct ggagttggca tgcgttcaag gtaatgattt
     7741 tccctctgat attggttatt gaacttgcat tctttgctgg aaatataccc aagttgttaa
     7801 ctgggggttg gatccctatt ttaatcgcgt ttctaggatt tgtagtgatg tatacctggc
     7861 actgtggctt tgagaaacta cgtgaattgc atcatagaga tgctctgatg gatgctttta
     7921 tcattgatga attaaatcaa aataaaattt caagacaatc cggaatggga ttatatatta
     7981 tcgatccata tgattgtgaa ggggaaagcc ttcttcacca tcttcgcctc aaccgtattt
     8041 tttttgagaa tatgattttt gtaagtatca aaattgaaaa taaaccttat atccctattg
     8101 aagataaatt tgagcttata aagaaagctg aagggtttta tttaattttt attcattatg
     8161 ggtttactga aaatattaat ttgcctaatg aattagatga aatgtttaaa agagtatact
     8221 taccttttga gataattaag aataaattaa tctattctat tgagatcgtt tttgttgaaa
     8281 tgactagaga aaggcaaaaa cacatgtaca tgtggcaaaa gcaccttttt tctctcatga
     8341 tacgcaatgc tgtgccagat attcagtttt atcgattacc ttacaataaa actattgcaa
     8401 tagggactta ttatcaatta tgagcccatt caaagaggcg atattgggtt tttaagtggt
     8461 taaggatagt gaaatgaaca aagccgattt acataactgc gtgttattta agggaataga
     8521 tgaaaagtac attgatgatt ttttggaaaa ttgcgaggaa gttgaacttc cagaaggtga
     8581 gtttcttttt catcaaaacg aaattggtga ctcgatgtat attgtcgaac agggtgaatt
     8641 acaaattata ctggatcaaa attcgacaga ttcgaatcct caggaggagc aagtgattgg
     8701 agtacttaaa agcggagcgt tgtttgggga attatgtgta tttggacaac taaaacgagc
     8761 cgcatcaatc cgtgccctgg tagattgtag attattaaaa atcgaagggg aggatttcag
     8821 gattcgtatc tactcaaaag atttggacgc attgttgatt tgttataata tcgcaaaact
     8881 attaagccag cgctttatta ctcttttaag cttacgtcct tctctgaata tgtaaattgg
     8941 aatagacctc attaaatgac gcgctcaaat agattcaaac ccacggaacc atagttcgaa
     9001 gctgatgctc tgataattcc ttataaatca atatattgtg agtcgtcaaa ttcacttgaa
     9061 atggtttatt accgcgaggt gtcgctatta tggagtctgt tatcaactag ctaaaaaaat
     9121 atttccaata tcgagcactc aatgcatctc gctgtcttta attttattat caattgtttg
     9181 gctgggatag tggcttaatt ccttaagcta aaaaacatta tttaaatagc caaagaattt
     9241 ttatggctca gttatatcga attcacgttg aaaatttaat gtttataagg tgccctggca
     9301 ggcagctcag gtgactggct ctttttccat aaccaatacg atgcatacac agaaataaat
     9361 gagcataaaa taacgggtat agcagcccat atataattat catgaaatag atcaagtaat
     9421 tcgccaaaga gcagggataa aatcattccg acacagatct gtcccatgtt caaaatgcca
     9481 atccaggtac caatcatcgt gggcttaaca tagtctttaa ccaaagaaaa accaattgtc
     9541 actgcaccag taaaaaaacc aactatggca taaaagatca tttttaggtg taggtcatga
     9601 agataaagga tgttaaatgc caataaaata ctttcaatca atgcggcata aaacatgatt
     9661 ggaatgacgt tctttacttt atgagacaat actccaaaaa aaggtgcgcc gactatgtac
     9721 cctaaagtgg aaaagccatt aacaagggtt gcgtctatct ggctaagatg ataatgctgt
     9781 tctagaaagt caattactaa aattaagagt tcattttgct gtactctcaa ttttcagtag
     9841 catgttttca caaatatcct tatggaaact tgaataaaac gcttgttaaa tccagttttc
     9901 atttgcagta aaggcaatgg ttagccaacg ttgtggcgta tcttctccaa tagccaacgc
     9961 gatttcatta cgaattttat ccagggtttc aatgctcgaa attgggtaat ccataggtac
    10021 tactatatgt atttcaataa attgtgcgcg tcctattttt gcaacgtagc ttgtataagt
    10081 cttgaaatca tgctgtttaa taagctcatc taaaaattct cttattcttt catcaagacc
    10141 cacaggagcc atgcgaaata tgtctcgcat ggcatcacgg actgcggcca taggtacaaa
    10201 aatcagaaat gcggtaagaa ttgctaaaat aagggggtca atgtaagggg taaaatatcc
    10261 ataggcccca ccatgcaaca aggctgcgat gccaaatgcc aataggagag aagtggaaat
    10321 caaagcagac attaaccaac tttgaatgtc tagtcgcaaa aattcggact taattttgcg
    10381 gtttgtttta actaaataaa aatacatgcc tgttgataaa aaaaacacca aaagtgcaaa
    10441 cacaaatgca taatcaaaat tgagttcatg tccaccagac atcaaactgc caatcgcgtt
    10501 taccagtgca taagtacaaa gaagtattag gatactgcca ttcaaaacaa gaaccatagg
    10561 ttcaatatgc cagtaaccat attgaaatct ccgattcccc ttacttgtta aaagacgggc
    10621 aacaaaaaaa gcaaggattg atataacggt atccaccata ttaaacatac cgtcaaagac
    10681 gatagccaat gaaccggaca atagcccaaa taaaatacct accacggcta agagaaaggt
    10741 aacagcgatt gatattttaa gagctcttcg ttcatgaagt cccttttgta ttgtcatttg
    10801 agtgatatcc tgattttaac aatgctttat ctacttatac tattgcttca acagccacta
    10861 ataaatccat aggcttgtat tattaggcct tttacataat aactgcttct tttttcaatt
    10921 gttgcagtaa atttttcaca tggtttagaa aaatcaattt attttacgta cattaacttt
    10981 gcgtcctttt aattttccac tttgtaaata ttggtaggct ttatctgctt ggctgtggtg
    11041 aattgcaaca taggagtaca tagcagtgat gttaattttg ccaattgtgt tgcttgctaa
    11101 tcctgcatct ttggttaatg ccccaaggat gtcaccaggg cgaattttat cttttttacc
    11161 ggaagcaata cagagtgtaa ccatttctgg cactaagcga gcagtgttgt gattttctaa
    11221 ttcattaatg tttctccaat gaatcgggtg cgggtagtta tcttcaatgg cgcaaattcg
    11281 ttgtgcatct gcaggagtta caatgcttaa ggcaatgcct ttactcccgg ctcgtcctgt
    11341 tcgaccaata cgatgaatat ggacgtcatg atcaaaagcg agatcaaaat taatcactgc
    11401 ggaaagttct tttatatcaa gtcctcgtgc tgcaacatca gtggcgacaa gaatagaaca
    11461 actttggttg gcaaaacgca agacagcaag atcacgttcg atttgttcca tatcaccatt
    11521 taaagcgatg gcactgaagc cttcattgat gagctggtct gtgacttcta ctgtttcttg
    11581 cttggtatta cagaagatta acactgaaac gggccgatag tgtaacagta atgattttaa
    11641 taagggaaat ttctgagtat gtctcgagac ttcataaaaa tgttgttcaa tatcaatttc
    11701 ttcaggagga gttaccacat gaacttcttt cggatccttc ataaattgtt ttgaaagccg
    11761 tttgatttct tcaggataag tcgcagaaaa aagcagggtt tgtcgttgtt tgggacaaac
    11821 cgaaataatg tttttaatgt catcaaaaaa acccatatca agcattctgt ctgcctcatc
    11881 caaaactaaa gcttttacct gagataagtc taaagaggcg ttttttaaat gcttaagtat
    11941 tctccccggt gttcctacaa taatatgagc tccatgccgt aatgaatcaa gctgaggctg
    12001 cattggtatg ccaccagata aattaataat tttgacatta ggcatcaaac aggctaatcg
    12061 acggatagct tggcttactt gttccgcgag ttcacgggta gggcaaagaa ccaaggcttg
    12121 tactgcaaaa aaagaaattt ttaaattatt taataaacac aaagcaaaag cagcggtctt
    12181 tccactccct gtttttgctt gagctattat atcttcattt ctaagaatta tgggcaggct
    12241 ttgcatttgg ataggggtca tattttcata atttgaagaa gcaagacttt ttattaattc
    12301 ttgacgaagg gggaattgag agaatgatag gtgttggtcg ttgtgttctg attgaatcat
    12361 ggaaagtgct tattgttata ataggtgaat agcttagcac ttcttagtgc aattttaaat
    12421 caatttattt tggacaagct taatctgatg cttacaattg tcttttctac ttgaatccgt
    12481 gtagatcaaa ataacattaa aatcatctca aagacatcaa cagagagtga ttttaatgtt
    12541 tctatcagat gtatcattct acaaagagat ataagtaaaa ataatattag ctacttaaat
    12601 taaagggctg ttatattttg tgcttgaaga ccttttgctc cttgcgtcac aatgaattgt
    12661 actcgctgac cttcagtcag ggttttaaag ccagaacttg aaatttcttt aaagtgagca
    12721 aatacatccg ggccgccttc ttgctcaata aaaccaaatc ctttgatctc gttaaaccat
    12781 ttaacgacgc catttactgt tttagacata agtatcctta agtatttatt ttattatagt
    12841 gcctgtatag gcgaacctag ccggaaaccg gtcaagaatt aaaaactacc gaacgaggga
    12901 ttaagtgaat aaaacgacga tttggaaagt ataaaaatag acattttcta gctgagatca
    12961 ggatactatt ctttaatgct ataagtcaat cttattgtcc ttattgagac atagataaca
    13021 aaatattccg gagacatagg ttacactcaa aggtatttct agagaagaga gatgccatgg
    13081 taggagtgta ataaagtgga tgaaaagata aaatttgtag caaggttatt agatggcgaa
    13141 caaatgtctt ctttatgcca agaattcggt atttctcgaa agacaggtta taaagatata
    13201 tgttcgatat aaagaaagtg gattagaagg cttgaatgat cgtagtagga ggcctcatcg
    13261 ctatgcgaac caattacctt ttcaacttga gaaggaaata ttaaaagtaa agaaagagaa
    13321 accgagctgg ggtacgccga agatacgaga aaagatatta agacaatatc cagatgtaaa
    13381 accgcctgca ataagcacca ttcatactat tcttgataaa tatggcttgg taacaaaacg
    13441 taagagaagg cattataaag cggagggtac caagttaact aatggaaagt cacccaatga
    13501 attatggtgt gcagattata aaggtgaatt tcaattagga agtaaagagt attgctaccc
    13561 attagcgata actgatttta atagtcgcta tttacttgca tgcgaagggc tatcgataac
    13621 aaaggaacaa tatgccatca ctgtatttga gcgtgttttt aaagaatatg gcctacctaa
    13681 cgctattcgt actgataatg gtgttccttt ctcttcagta caagcattat tcggactcag
    13741 taaactatct gtatggtggc ttcgattagg cattagtatt gagcggatca ggcctggaaa
    13801 tccgcaagaa aatggtcgcc atgagcgcat gcatttaacc ttaaagaaag aaacaacaaa
    13861 gccatcagga gaaaacttct tacaacagca agagaagttt gaccgattta ttgatgagta
    13921 caataatgaa cgcccgcatc aagctttaga tatgcgttat ccaggcgagg tatatgtccc
    13981 atcaaacaag gagtacaaag gtcttcctga ggttgattat ccatttcatg ataaaatgat
    14041 agccgtaact cactgcggta ggttgtgttt aaaaggcaaa aaataaacct caccagtgta
    14101 ttggcaggtc agagtgttgg tattaaggag gtagaagaca atatttggtt agtaagtttt
    14161 atggaatatg atttaggtta ttttgatgag cagacatgta aattggagcc tttacccaat
    14221 ccttttgggc ctaaagtgtt acctatgtct ccgatataat ttgtaaccga tgtcttcgaa
    14281 atagacctat tttaaatggc gcgctcggag ggactcaaac ccccgacacc ttggttcgaa
    14341 gcctgatgct ctggttttaa attcgttata aatcaatacc ttgtgaggcg tcaaattcaa
    14401 caattactgc ctaaaactgc ctactacagc gtagtaccgc cacagttgag acacgttttg
    14461 gtcacagaag aatcttaatc attttgttta aaattaaact taatatgtgc tattccagca
    14521 tcaggaaata aggatttttt cttaagttat agatgtctat taaaatccat agattgatgc
    14581 aacactcgaa taacagaaat gccgtaatcg gtagttttga aaaaaataat gtgagagcca
    14641 acattaaatg cgcgtaattc tggacgaata taatcacatt ttcgagcaat taagggatca
    14701 tctgcaaggc gctgaaaact taaatctata gctaaaatat aatcctctgt tcgtttgata
    14761 ccaaactcat ttgtgctata taaatagatg gactcaagat cttctaccgc ttttgggtaa
    14821 agacgatatg ttttatttga catgggattt tttcttcatt ttcttaagaa aatcatgtgc
    14881 atcccatgtt tcaggcttcc cgctagctgc cccttcatct ataaggtgac gcaactgctc
    14941 tagttttgat tcagcttgtt tttcacgaag taaacgcaac gactctctta atacttcgct
    15001 attacttcta taatcccctg agcgtacaag tgattccacg taattacgaa gttcattacc
    15061 caaatcaaca gtcattgtac gggtcatatt tgcctctatg ttgtaagaat aaatcttact
    15121 tatattatat aagacattgt cttacatttc aaaccatgta attagtccct cggacactaa
    15181 gattcttatt ccttatgatt gttaatggtc tttggtggtt tttactttct cctaaagggg
    15241 gcactgcaga ttaattttga gatatctcat taatttatta gtggctaaaa aaatattctg
    15301 cccctcttaa tttgtgtcct ttgttcgcca gccttgataa ggtttgttta aacaaactaa
    15361 cagcacctaa aagcaaacct ttgctctcta tattaccacc ctaattttct aatttattgc
    15421 tattggttcc ctcactaaat gcctcttttt ttttcacttg cttccataat tgagtgagag
    15481 tttttatgtt ttttatattc ttgccagcct tgataaggtt ttttaaacaa aacaacacca
    15541 actgtatctt taaaagtaaa tccattactc tttatcgtgc cagcccgaat ttctaatttt
    15601 ttgctattgg ttacctcatt aaacgcttta ccttcttttt tcgcttgttg gtactgtaaa
    15661 atatgttcac tggttataac gtggatgttc gtattgtatg ccttggccaa cctttctaaa
    15721 gtagacatta tgtctcgctc attggtacct aaagctctca actcatcatt tccatattta
    15781 taacctaagg cattttcaat ttgatttaat ttaaaagcag atttgcaaaa tttttcagtg
    15841 acgcattgtg ccagataggg ataatctttg cgggcaattg acattaagta ggaatgaaaa
    15901 taaaaagggt ccatcccata aatatactgc gaattatgtt ccatattatc ggtaataatg
    15961 gctggtgttt tacgcaaaat taaaggatgt cgcagattca tttttgcgtt atcacccact
    16021 gcaacggtat cgattacact atcccataaa ggaaggtcat tttgggtttt aacttttgcg
    16081 agtatatttt ttaattcgac aacgcttacc cccctatatc aagaaggcct agctcaacag
    16141 ctatcatgat taaattgagc tgtagacgat gtgtttcttt gccatggaaa aataaagggg
    16201 ataatttatt ttcttcgctc cataattggt ttttttctaa aaccttatcg gcttctttct
    16261 tatcgacaaa ccctacaaaa gtagggaccg gtctttgaga gagattttcg tcgaaattga
    16321 aaccatgttt ttgctctcga taaagcaaga ccttgcttag aagattattg gttttattcg
    16381 aaattaattc ttcattagga accggccaca gaagattcat aaagcgacaa aattgaattg
    16441 attgatttag ctttagctgt ttgtccgctg ttctagttga attttcaaaa tcccataaaa
    16501 atttgacctc ttgctctaat acagccagtg cttgagttat gagtgagggg tttgataaaa
    16561 atacagatac attggccaat acaggggcat gccgatgggc atccttaggt aatcttgatt
    16621 ggtaatagaa aagata
//
LOCUS       DASLOE010000054        16371 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.054, whole genome shotgun sequence.
ACCESSION   DASLOE010000054 DASLOE010000000
VERSION     DASLOE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16371
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.054"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..499)
                     /locus_tag="U6309_001409"
     CDS             complement(<1..499)
                     /locus_tag="U6309_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0393 family guanine-nucleotide exchange
                     effector"
                     /protein_id="HEN5504857.1"
                     /translation="MLSSTKEYLQALRDGKYLLFLQWPKFIAEYYGKNDKNQEADEMV
                     SLLIFEWLNNGFCLDDIKKFAILYAVHEMESRPLREGLSYALTTISIALFPCMVYLTN
                     NLQEHYITSKKLSSKEVLQLMAMNNAYLEKQRFVEFLGQEQDKFFTWVKEADSSAVSK
                     AFDQIY"
     gene            648..929
                     /locus_tag="U6309_001410"
     CDS             648..929
                     /locus_tag="U6309_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504858.1"
                     /translation="MKNKLAISILLFLLPTAAAMANCDLTKFRWDCDLPVQVKPSPGA
                     SSLVYCGSSYGYITKQQYDILARYQRASVNMVLDINGEYIDSPCIGAER"
     gene            1116..1823
                     /locus_tag="U6309_001411"
     CDS             1116..1823
                     /locus_tag="U6309_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEN5504859.1"
                     /translation="MHIQIDGIPIEILRKPIKNLNLRIYPPDGLVKVSVPLKYSEQLI
                     KQQLQEKSEWIRAQCERIRARSLYREPSLQTGAAIPFKGKNYLLIVEEHHGPSQILIK
                     DELIYYYTKPDLTPQQKLNILERWYRKEMNDVIPRLIQQWEAIMKIKINQWGIKKMKT
                     RWGSCNPRAQRIWLNLNLIKKPLECLEYVLVHEMVHILEASHNKRFYALMAQFMPKWQ
                     EYQKILEGTSNASTFAR"
     gene            1909..2547
                     /locus_tag="U6309_001412"
     CDS             1909..2547
                     /locus_tag="U6309_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEN5504860.1"
                     /translation="MFNPDLIAHNICTQGFHIIDEFLEETHYCSLRDMAYELKQQNLF
                     RNAKIGQNLQAQQNDKIRSDQIFWIDADSPYPPIQAYLKKLISLGNILNQTMYLGLAE
                     FETHFAIYLPGTFYRRHIDQFESTKTRKISFVYYLNDTWQKEFGGELLLYNIEDELIQ
                     NIPPLGNRFVCFDSQLPHEVHPTQRTRFSMTGWMKTRSTSVLADNKLTQITQ"
     gene            2636..3694
                     /gene="vipA"
                     /locus_tag="U6309_001413"
     CDS             2636..3694
                     /gene="vipA"
                     /locus_tag="U6309_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector VipA"
                     /protein_id="HEN5504861.1"
                     /translation="MKHYDSMDQELITMPISNAFLKLFGEFVDNYARNGTPSFYHWAK
                     EQNHEVINKFFHELLEIIKSNTETFHDVSIIEHLNATVLLSAKTNPYLLDCWISFITK
                     YNAETNSFTPIGVSMHIKVGPEGTPSERIIRELKESLVITQQAFILAQKANATEVDSL
                     KRQLEQTKQENERLVTQNQFLKDQVRAEKTFADLNPQINNARQQLSALTELIQTLSEI
                     TNAKPKIETVSSLSIPAATENKSELLKQNSCSVAPVSKEVTPAISQEPKKRPVSPPPN
                     DMDGQALSPNIPPKEDVSIKVTITSIPKPNSTQPVAKNSGFFSGDGFLKELNAKIKER
                     KERPKDESHTTPVEKKNL"
     gene            3925..4839
                     /locus_tag="U6309_001414"
     CDS             3925..4839
                     /locus_tag="U6309_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN5504862.1"
                     /translation="MHALEIKQLSKTYSNGVEALKGIDLTVNTGDFFALLGANGAGKS
                     TTIGLITSLVNKTSGNIKIYGFDLDAYPEKAKSCLGLVPQEVNLNIFENCEQILLNQA
                     GYYGISRKKALPRAEALLEQLGLWDKRRSIVRHLSGGMKRRLMIARALIHQPKLLILD
                     EPTAGVDIEIRHSMWDFLARTNAEGTTIILTTHYLEEAEQLCKNIAIIDKGQIIKNTS
                     MKALLQTLRHQTFIFNTENPIDYLPDIHPFNVTPIDPTTFELRVDNNFSLNEVFAVLT
                     KQGIKIHSMRNKTNRLEELFMDLIKNGI"
     gene            4829..5602
                     /locus_tag="U6309_001415"
     CDS             4829..5602
                     /locus_tag="U6309_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN5504863.1"
                     /translation="MGFKYQLIALYTLVRREVVRMFRISSQVFLPPVITTTLYFLIFG
                     SLIGPRIGDIEGVDYSQFIAPGLIMMSVIINSYGNVSNSLFSVRFQKSIEEMLISPMH
                     NGLLLLGYVIGGVLRGLIVAILVFVISTFFLPVELDHLPMTLLVVMLVAAIFSLAGFT
                     NAMVARNFDDIMLIPTFILTPLTYLGGVFYSINMLPPFWQKISLFNPILYMVNALRHT
                     MIDQEEVSITLAMIIIFLMLIALTVGNMILLKKGVGFRE"
     gene            complement(5629..6648)
                     /gene="htpX"
                     /locus_tag="U6309_001416"
     CDS             complement(5629..6648)
                     /gene="htpX"
                     /locus_tag="U6309_001416"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEN5504864.1"
                     /translation="MGIADYHATAGDWRAQLRRNEKKTRRVIAVFFLVYIFVGLIVDA
                     FVLQGMYPHASLENIIHALITFKVVPIATLLMIAFAGISLLITYALYDRIMLLGTEYY
                     QITPETARSLQEKQLYNVVEEMKVASGLQYMPKIYIIEASYMNAFASGYSEKSAMVAI
                     TRGLMEKLDRAEMQAVMAHELSHIRHHDIKLTLMVAVLSNILLIVIDILFYSVIYKRD
                     RRGDNRLLMVIIILRYVLPIITVLLALFLSRTREYMADAGCVELMRDNEPMARALLKI
                     NQDHVQNAEQYAKAYGDTPHEQVRQASYLFDPSHIDPVKSLNNAFSTHPSIEQRLEAL
                     GFKRK"
     gene            complement(6659..7240)
                     /locus_tag="U6309_001417"
     CDS             complement(6659..7240)
                     /locus_tag="U6309_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEN5504865.1"
                     /translation="MKTFFIVILIIIALAFIWAISIYNTLIGLIEAINNNKKQIDIQL
                     DRRFKVFESLIEAVKKYMDYEKTTLKDVVALRNQAQAAKDAGDEQGRIAAENGISKIA
                     SNLNLVFEQYPDLKASQNVMQLQEEIVNTENKLAYAKQAYNDGIERYNAKKKSFFESM
                     VVAMFSSALDKEFIYWGLPEEQIQAKENYTVKF"
     gene            complement(7421..10276)
                     /gene="uvrA"
                     /locus_tag="U6309_001418"
     CDS             complement(7421..10276)
                     /gene="uvrA"
                     /locus_tag="U6309_001418"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEN5504866.1"
                     /translation="MHTITIRGARTHNLKNLDLELPRDKLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMEKPDVDSIEGLSPAISIEQKATSHNPRSTVGTIT
                     EIYDYLRLLYARVGEPRCPTHHTSLHAQTISQMVDQVLALPEDSKVMILAPVVRERKG
                     EHVQFLQQLQAQGYVRARIDGELYELDSPPKLGLRTKHTIEVVIDRFKVRPDLAQRLS
                     ESFENALNLADGLAIVSSMDGHFKELLFSSRFACSECGYSLSELEPRLFSFNNPVGAC
                     SSCDGLGVDQFFDPSRVVHDATASLAEGAIRGWDRKTTYYFSMLESLARHYGFDTSTP
                     FCDLSEAMQQIVLYGSGREIIEFQYHRPHGGYMVKRHSFEGIIPNMQRRYRESDSGMI
                     REELAKYLSSRPCQSCHGARLREEARHVFIDNKNLPEITSYSIEQAYQFFKELKLSGY
                     RGEIAAKINKEIVERLGFLVNVGLDYLSLARSAETLSGGEAQRIRLASQIGSGLVGVM
                     YILDEPSIGLHQRDNDRLLSTLMHLRNQGNTVIVVEHDEDAIRSADFVLDIGPGAGVH
                     GGEVVASGSPEEVMNNPASLTGQYLSGKEEIEIPKNRIPVNHDRMIHLKGVSCNNLQN
                     VDVSIPLGLLTCITGVSGSGKSSLINDTLYPIAANALNRASLMTIGEVKDISGLHLCD
                     KVIDIDQSPIGRTPRSNPATYTGLFTHIRDLFSGTPESRARGYQPGRFSFNVKGGRCE
                     ACQGDGLIKVEMHFLPDIYVSCDVCQGKRYNRETLEIHYKGKNIHEVLDMTVEDACAF
                     FAAIPVIARKCQTLMDVGLSYIKLGQSATTLSGGEAQRIKLARELSKRDTGNTLYILD
                     EPTTGLHFHDTKQLLTVLTRLREQGNTIVIIEHNLDVIKTADWIVDLGPEGGSKGGKV
                     IATGTPEMVAETPQSFTGQFLKQILSKSNELLLQKCK"
     gene            10371..10829
                     /locus_tag="U6309_001419"
     CDS             10371..10829
                     /locus_tag="U6309_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504867.1"
                     /translation="MKSLIKSLLFIVVFSINSSALYAANSNTRVIQWAQQTLMDTLSV
                     SYTTKSQDFATVRKNYSLNAWSGITGFLGGNMQSIREKQLTLHPVLLPDTQILSTGLY
                     LGMSYWIIRQSVAIDELNIKLTFTLTILGRSVAASSPYIVQSLSILLNTL"
     gene            10971..11522
                     /locus_tag="U6309_001420"
     CDS             10971..11522
                     /locus_tag="U6309_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEN5504868.1"
                     /translation="MPKCTHFILATALIFVTSTFYASNLRAIEQEVSDSVITAKITAK
                     FAESRHLNPLKISIDTEKGIVTLSGYAPDKQAFIDALQIAKDTKGVKAVHAKDLHIKK
                     VNTAFTDAFITAEVETAVLKAKILDDESIPLVGINASTTNGEVTLSGHVKSAGSIAAI
                     IKRVNEIPGVKKIVTNLEIQENT"
     gene            11519..11992
                     /locus_tag="U6309_001421"
     CDS             11519..11992
                     /locus_tag="U6309_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEN5504869.1"
                     /translation="MSPLTQFKFFLWFFSHFKVALIGYLKPKLIQLTDNEIVVRLPLT
                     RRSRNHLHSMYFGALAVGADIAGGLHGFYHAKQAKCKVSLAFKSFQAQFLRRPESDVY
                     FICTEGDVVKKMIEESKKSGERINKPIHIKAYINYLTHPEEIADFILELSLKVIK"
     gene            12187..12900
                     /locus_tag="U6309_001422"
     CDS             12187..12900
                     /locus_tag="U6309_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN5504870.1"
                     /translation="MIDLIIQTQPDDETCGPTSLHAIYLYYGLDLPLNEIIQGVDKSL
                     SGGTLTPMLGKHALLHGFRTTVYIYNLNIFDLTWFHNGEVNNSFLMDKLEAQMKYKND
                     PYITKASLAYLDYLKLGGTIRSKSLNVDLLKNYFNKKIPILTGLSATYLYRSAREYFT
                     PEGKSVYDDIRGEPCGHFVVLCGYDVKKRLVIVADPHAENPLSHNNYYKVSINRLINS
                     IMLGVLTYDANLLIIEPKE"
     gene            12904..14358
                     /locus_tag="U6309_001423"
     CDS             12904..14358
                     /locus_tag="U6309_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HEN5504871.1"
                     /translation="MQTVIVTDDLDSWSFLSELAPIVHALEYLSGDEYHQNKSFRVIN
                     LCRSYNYQSIGYYVSLLAQARDHKALPSVHSIQDVLNVSLSKLISQDTHEEIQHSLHD
                     IKGNEFVLSLYFGQNLAKRYATLAKQLHGLFPLPLIRFYLEKKKQWRIKTVVPLSLSD
                     VPEHHLEFMRQSAENYLSKKRVHQWRKKQRFHDLAILVDPSEPNAPSNKKALELFVSC
                     GEELGLNVDIIDKNEAKAIAEYDALFIRATTSVNHYTYRVSRRAAQENLVVIDDPQSI
                     IKCSNKVYLAELLSSHQIMTPDTLFISKYDKELPKIEFPCVLKKPDSAFSHGVVKIDD
                     EKSLQKSLNQFFKNSDLVVVQPFIPTEFDWRIGILDNKALFACRYFMAKGHWQIYDWH
                     CQEENKEGDSETLPLHEVPEQIIKIALKSTRLIGDGLYGVDIKSHGNKYYVIEVNDNP
                     NIDHGLEDKILGQNLYEQIMSVFLQRIRRKHGYV"
     gene            14351..15571
                     /locus_tag="U6309_001424"
     CDS             14351..15571
                     /locus_tag="U6309_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEN5504872.1"
                     /translation="MSDFPIFSVLGIEIEYMLVDKESLNVQPKSDLILRALAGHQVNE
                     IELGDIAISNELVLHVLELKNNGPKPLHVPVAEHFQKTIRELQPLLAQHNLLLLPTGA
                     HPWMDPNTETIRWPHGNHDIYNQFNSIFNCQGHGWANLQSMHVNLPYSNEEEFCQLHN
                     AIRLILPLLPAIAASTPFLDGQATGLLDSRLYFYSRNQQRIPSISGDIIPEFIRTERE
                     YQEQILEPMYRDISPYDPEGILQYQWLNSRAAIPKFDMKAIEIRILDSQECVEADIAI
                     AKAINAILKYWQSTSNFYLDNPCDTKSLKYVFDETIKNGLSCHIDNKKLLEQWQLPLQ
                     EMSVNTIWSLLIERISHELDRKQQLALELILKQGNLSERILKACSSCVNHDSLKQVYT
                     QLAHCLLRNEQFTP"
     gene            15552..16295
                     /locus_tag="U6309_001425"
     CDS             15552..16295
                     /locus_tag="U6309_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEN5504873.1"
                     /translation="MNNLPPDIMKNIALLISCEHAVNTVPYEYQTLFEPHKDILESHR
                     GIDFGALEIAQSIHKNNHCSLFQATCTRLLVDCNRSINHPHCFSEITKNLSSDKKKKI
                     LDEFYFPFRNRVMDHIHQLISQGLQVWHLSTHSFTPVMNNIIRTTDIGLLYDPRSNSE
                     KILARQWQKEIKILHPQLKVRMNYPYKGISDGFTTSLRKKYSHHEYIGIELEVNQKLV
                     FHNHSLNALKNTLPLSVSNTIKAYQKSER"
BASE COUNT         5329 a         3359 c         2868 g         4815 t
ORIGIN      
        1 aataaatttg atcaaacgct ttagaaaccg ctgaagagtc tgcttcctta acccaagtaa
       61 aaaatttatc ttgctcttgc cctaaaaatt cgacaaaccg ttgcttctca agataagcat
      121 tgttcatagc cattaattgg agcacttcct tacttgataa ttttttcgaa gtaatatagt
      181 gctcttgcag attatttgtt aaatacacca tacagggaaa aagagcgata gaaattgtag
      241 tgagtgcata ggataatccc tctcttaaag gcctggactc catttcatga acggcataca
      301 aaatagcaaa ttttttaatg tcatccaaac aaaagccatt atttaaccat tcaaatatga
      361 gcaagcttac catttcatca gcttcttgat ttttgtcgtt cttaccataa tattccgcaa
      421 taaattttgg ccactgcaga aatagaaggt attttccatc acgtaaagct tgaagatatt
      481 cctttgttga tgaaagcata gttatccatt accaaaatct gattattcaa tttataacat
      541 taacaatact aaaaatcttg tgaaaaatta tttcatgtta aacaacatac gcacatcaat
      601 tgttatccta tacttaaaat aaaacgacaa atcaggtggg gaattccatg aaaaataaat
      661 tagcaataag tatattatta ttcctgttgc caacggctgc tgccatggct aattgtgatt
      721 taaccaagtt tcgatgggat tgtgatcttc cagtacaggt taagcccagt ccaggtgctt
      781 cctctctagt gtattgtgga agctcttacg gctatatcac aaagcagcaa tatgatattc
      841 tggctcgcta tcaacgcgcc agtgtcaata tggttttgga tatcaatggt gaatacattg
      901 atagcccgtg tataggagcg gaacgttaaa atttttgcag caataactta cagtagacaa
      961 tgagaaaata aaaaggaata ttattatttc cagcaagttg cttattcaaa atcaaaaatc
     1021 tctggacttg tgggttttca atgaactcaa gtatcaatat cataaatatt acttcttctg
     1081 tataatcgaa aaacaaatca ataagctatt ttgctatgca cattcaaatt gatggtattc
     1141 ctatagaaat actgagaaaa cccattaaaa atttaaattt gagaatttat cctcccgatg
     1201 ggttagtcaa agtgagtgtc cctttaaaat acagcgaaca gctaataaaa caacagctac
     1261 aggaaaaaag tgaatggatt cgtgcccaat gcgagcgaat tcgcgcacga tctttgtatc
     1321 gtgaaccatc acttcaaaca ggtgcagcca ttccattcaa aggtaaaaat tatttattaa
     1381 ttgttgaaga acatcatggt ccttctcaaa tcctgattaa agatgaattg atttattact
     1441 atacaaaacc cgatttaact ccacagcaaa agctgaacat tctggagcgc tggtatagaa
     1501 aagaaatgaa tgatgtcatt cccaggctta ttcaacaatg ggaagccata atgaaaatca
     1561 aaattaatca atggggtata aaaaaaatga aaacccgttg gggttcgtgc aatcctcgtg
     1621 cacaaagaat ctggctcaat cttaatttaa ttaaaaagcc tttggagtgc cttgaatacg
     1681 ttcttgttca tgaaatggtg catattttgg aagccagcca taacaaacga ttttatgcac
     1741 tcatggcaca attcatgccc aaatggcaag aatatcaaaa gatactggag gggacatcta
     1801 atgccagtac gtttgcaagg taatcatcga attcattacc tcattatttc caaaaaataa
     1861 ttcttggcgt acaatgcatt atcactttgc ctgattggtg tagacaggtt gtttaatccg
     1921 gacttaattg cccataatat ttgtactcaa ggatttcata tcattgatga atttctggaa
     1981 gagacacact actgctcttt acgagacatg gcttatgaat taaaacagca aaatttattc
     2041 agaaatgcaa aaattggcca gaacttacaa gcgcagcaaa atgacaagat tagaagcgat
     2101 caaattttct ggatagatgc agactcccct tatccaccca ttcaagctta cttaaaaaaa
     2161 cttatctctt tgggcaatat tctgaatcaa accatgtatc ttggtttagc agaatttgaa
     2221 actcattttg caatttatct ccctggaacc ttttatagaa ggcacattga tcaatttgaa
     2281 agtacaaaaa ccaggaaaat atcatttgtc tattacttga atgacacttg gcaaaaagaa
     2341 tttggtggcg aattgttact ctacaatatt gaagatgagc tcattcaaaa catccctcca
     2401 ttgggaaatc ggttcgtctg ttttgatagt caattaccgc acgaagttca cccaactcaa
     2461 cgaacgcgct tcagtatgac aggctggatg aaaacacgct caacctccgt actggccgac
     2521 aataaactga cccaaataac tcagtagcac tttatccgct gttctataaa aaatgatgaa
     2581 taattatttt ttaacttcac aacagctatt gaactttaat ttaagttgat attaaatgaa
     2641 acactatgat tccatggatc aggagttaat taccatgcct atcagtaatg cctttcttaa
     2701 gttattcggt gaatttgttg ataattatgc gcgcaatggc accccttcat tttatcattg
     2761 ggcaaaggaa caaaatcatg aagtcataaa caaatttttc catgagcttt tggaaattat
     2821 taaatcaaac acagaaacat ttcatgatgt cagtatcata gagcacctta atgctacagt
     2881 actattatca gcaaagacca atccctactt actcgattgt tggatttcat ttattactaa
     2941 atacaatgct gaaaccaaca gttttacccc aataggggtt tcgatgcaca ttaaagtcgg
     3001 gccagaaggc acaccatccg aacggataat aagggagctc aaagaaagtc tggtcattac
     3061 gcaacaagcg tttattttag ctcaaaaggc caatgctacg gaagttgatt ctttaaaaag
     3121 acaacttgag caaacaaagc aagaaaacga gcgcttagtc acacaaaatc agtttctaaa
     3181 ggatcaagtc agggcagaaa aaacatttgc agaccttaac ccacagatta acaatgctcg
     3241 gcagcaactc agtgctttaa cagaattaat acaaactcta agcgaaatca caaatgccaa
     3301 acccaaaata gaaactgtat cttcgttatc cattcccgct gctacagaaa ataaatctga
     3361 acttctaaag caaaattcat gctctgtcgc tcctgtatcc aaggaagtta ctcctgcaat
     3421 cagtcaagag ccgaaaaaaa gacctgtttc accgcccccc aatgatatgg atggtcaagc
     3481 attatctccc aatatacccc ctaaagaaga tgtgtctata aaagttacaa taacttccat
     3541 acccaaacca aattcaaccc agcctgttgc aaaaaattct ggtttctttt caggcgatgg
     3601 ttttttaaaa gaattaaatg caaaaattaa agaaagaaag gaacgcccaa aagatgagtc
     3661 acataccact cccgtcgaaa aaaaaaatct ctagttaagc caacttctgt tcctcctgaa
     3721 tataaggcac tcttagctaa aatggaagaa attagaaatg cagtaacagg gaaatatcct
     3781 cataccgatg attcatcgcc agagtcaact cctccttcat ccccagaatt aactcgttgg
     3841 aagtgaggga tgaattaaaa tccatagtct actgtagaat aaaaattatg gtgtataata
     3901 gcgtttttac ttaagtaata aatcatgcat gccttagaaa ttaaacaact ttcaaaaacc
     3961 tactcgaatg gagttgaagc attaaaaggc attgatttaa cagtgaatac cggtgatttt
     4021 tttgccctgt taggagcaaa tggcgctggc aaatcgacta ccattggctt aatcacatct
     4081 ttagtcaata aaacctcagg aaacatcaaa atttacggct tcgacctgga tgcttatcct
     4141 gaaaaagcca aatcttgtct gggacttgtc cctcaagaag tcaatctgaa tatttttgaa
     4201 aactgcgagc aaattttact gaatcaagct ggctattatg gcatttcaag aaaaaaggct
     4261 ttacccagag cggaagcctt gctagaacaa ctagggcttt gggacaagag acgttctata
     4321 gtacgacatt tatcaggtgg aatgaagaga aggcttatga ttgccagagc actgattcat
     4381 caacccaaat tactgattct ggatgaaccc accgccggtg ttgatataga aataagacac
     4441 agtatgtggg attttctcgc gagaactaat gcagaaggaa caacgattat cctgacaact
     4501 cattacctgg aagaagcaga acaattatgt aaaaacatcg caataatcga taaaggtcaa
     4561 attattaaaa atacctcaat gaaagcctta ttacaaacct tacgccatca aacctttatc
     4621 tttaacacag aaaaccccat tgattatttg ccggatatac acccgttcaa tgtgactccg
     4681 attgatccaa ctacttttga attgcgtgtt gacaataact tcagtttaaa tgaagtattc
     4741 gctgtattaa caaaacaggg aataaagatt cacagcatgc gcaataaaac caaccgatta
     4801 gaagaacttt ttatggatct gattaaaaat gggatttaaa tatcaactta ttgcccttta
     4861 taccctggta agacgtgaag tggttcgtat gtttcgcatt tcgagtcaag tgtttttgcc
     4921 gccagtaatc accacaacgt tatattttct aatttttggc agtttaattg gacctcgtat
     4981 tggagatatt gagggcgtgg attattcaca attcatagca cccggattaa ttatgatgtc
     5041 cgttattatt aattcctatg gaaacgtctc taattcacta ttcagcgtaa gatttcaaaa
     5101 aagtatagag gaaatgctga ttagtcccat gcataatggg cttttattgt taggttatgt
     5161 gataggaggc gtgctgagag gattaatcgt cgcaattctg gtttttgtta tctctacctt
     5221 ttttctgcct gttgaattag atcatttacc aatgaccctt ctagtcgtca tgcttgttgc
     5281 agccatattc tcactggctg gatttaccaa tgccatggtc gctcgaaatt ttgatgacat
     5341 catgctgata cccaccttta tcctgacccc tcttacttac ttgggaggag tcttttatag
     5401 tattaatatg ctgcccccct tttggcaaaa aatctctctt ttcaatccaa ttttatacat
     5461 ggtaaacgct ttaagacaca ccatgatcga tcaggaagaa gttagtatta ctctggctat
     5521 gattattatt ttcctgatgt taattgcttt gacagtaggg aatatgattc ttttaaaaaa
     5581 aggggtgggg tttcgcgaat aaaaaaccgg attccctgca aaatggctct atttgcgttt
     5641 aaaacccaat gcttccaggc gctgctcaat gctaggatga gttgaaaagg cattatttag
     5701 cgattttaca ggatcaatgt gagaaggatc aaaaagatag gaagcttgcc ttacctgttc
     5761 atgaggagta tctccataag cttttgcata ttgctccgca ttctgaacat ggtcttgatt
     5821 aattttaagc aaagccctgg ccataggctc gttatctctc attaattcaa cacaaccggc
     5881 gtcagccatg tattctctgg ttcggcttaa aaagagagct agcaaaacag taataatagg
     5941 taaaacatat cttaagatta taataaccat taacaagcgg ttatctcctc ttctgtctcg
     6001 cttgtaaatt acggaataaa acaggatatc aatgacaatt aataaaatat tactgagcac
     6061 ggcaaccatg agagtcaatt taatatcatg atgtctgata tggcttaact catgggccat
     6121 tacagcctgc atttcagccc tgtccagttt ttccatcaag ccacgagtaa tcgcgaccat
     6181 cgccgatttc tcactgtaac cactggcaaa agcattcatg taactggctt cgataatgta
     6241 aattttaggc atgtattgca agcctgatgc aactttcatc tcttcgacaa cattgtacaa
     6301 ttgtttttct tgtaagctac gagccgtttc tggcgttatt tgatagtact ccgtccccaa
     6361 taacataatg cggtcataaa gagcataagt aattaacaaa gaaatccctg caaatgcgat
     6421 cattaataaa gtagcaatag gcaccacttt aaaagtgatt aaagcatgaa taatattttc
     6481 caaagaggca tgagggtaca tgccttgtaa aacaaacgca tcgactatca atcccacaaa
     6541 gatataaact agaaaaaaga ctgcaatcac ccttcgggtc tttttctcat ttcgcctcaa
     6601 ttgagcacgc caatctcctg ctgtggcatg ataatctgca atccccatgg tatatagctc
     6661 aaaatttcac agtgtaattt tcttttgctt gaatttgctc ttctggaagc ccccaataga
     6721 taaattcttt atccaaagca gaagaaaaca ttgccacaac catggattca aaaaatgatt
     6781 ttttcttggc gttataacgt tctatcccat cattataagc ttgtttcgca taagctaact
     6841 tgttttctgt attaacaatt tcctcttgta actgcatgac attttggcta gctttcaaat
     6901 caggatactg ttcaaaaacc aaattcaaat tggatgcaat ttttgaaatt ccattttctg
     6961 cagcaattcg accttgctca tctcccgcat ctttagcagc ctgtgcctga ttacgtaaag
     7021 caacgacatc ctttaatgtt gttttttcgt aatccatgta ttttttaact gcttcgatca
     7081 gtgattcaaa aactttaaat cgcctatcca attgaatatc tatttgtttt ttgttgttat
     7141 taattgcttc tatcaagccg attagagtgt tatagatgct aattgcccaa atgaatgcga
     7201 gtgcgataat aattaaaatt acgataaaaa atgttttcat cttcctatcc tgtgaatggt
     7261 tccttcccta atttatagct cgaaatttca gaaaataaca gctaagataa gaaaaaagaa
     7321 acaactggat aacatgtttt ttgtcgtaga aagagattca tgttgcctga aaaacaagct
     7381 gcatggaaca tcagatataa agacaaatat aattaatgaa ctatttgcat ttctgcaata
     7441 gaagctcatt tgatttagat aaaatttgtt ttaaaaactg acctgtaaaa gattgagggg
     7501 tttcagcaac catttcgggt gttcctgtcg caataacctt ccctccttta ctgcctccct
     7561 cagggccaag atcaacgatc caatcagctg tttttatcac atccaaatta tgttcaataa
     7621 tgacaatggt atttccttgt tctcgcaggc gagtcaacac agttaataac tgtttcgtgt
     7681 catgaaagtg taatcctgtt gtgggctcat caagaatata cagtgtattt ccggtatccc
     7741 tttttgataa ttctctggcc aacttgatcc tttgggcttc tcctccagag agagtagttg
     7801 cactttgtcc aagctttata taagacaaac cgacatccat taatgtttgg cattttctgg
     7861 caataacagg aatggcagca aaaaaagcgc acgcatcttc gacagtcatg tccaacactt
     7921 catgaatatt tttgcctttg taatgaattt ccaaagtttc cctattgtat cttttgcctt
     7981 gacatacatc gcaagaaaca tatatatcag gtaaaaaatg catttcaacc ttgataagac
     8041 catcgccttg acaagcttcg caccggccac cttttacatt gaaactaaag cgcccaggtt
     8101 gataccctcg tgctcttgat tccggagtcc ctgagaataa atctctgata tgagtaaata
     8161 aaccagtata agttgccgga ttggaacgcg gtgttctgcc aattggactc tggtcgatgt
     8221 caataacctt gtcacaaaga tgcagtccag aaatgtcctt aacctcacct atagtcatta
     8281 agctggctct atttagtgca ttggcagcaa taggatataa agtatcatta attaaactgg
     8341 atttacctga accagaaaca cctgtaatac aagtcaacaa acctaaagga atactgacat
     8401 caacattttg taaattattg caagacacgc cttttaaatg aatcatccga tcatggttaa
     8461 ccggaattcg attcttggga atttctattt cctctttccc agacaaatat tggccggtga
     8521 gagatgcggg attgttcatg acctcctcag gagaaccgct ggcaactact tccccaccat
     8581 gcactccagc cccaggccct atatcaagaa caaaatcagc gcttcggata gcatcttcat
     8641 catgttccac aacgattaca gtatttccct ggttgcgtaa atgcattaaa gtactcaaca
     8701 atcggtcgtt gtcacgttgg tgcagcccta ttgaaggctc atccaaaata tacatcaccc
     8761 ctacaagccc tgaacctatt tgactagcca ggcgaatacg ttgagcttct ccgccagata
     8821 gagtctccgc gcttcgagcc aaagacaaat aatccagccc gacattcacc agaaacccca
     8881 gccgctcgac aatttcctta ttgatttttg ctgcaatttc acccctatat cccgataact
     8941 tcagctcttt aaaaaactga taggcttgct caatagaata gctggtgatt tctggtagat
     9001 tcttattatc aataaataca tgtcgagctt cttcacgtaa tctcgctcca tgacaagatt
     9061 ggcaagggcg tgaggatagg tatttggcaa gttcctccct tatcatacca gaatctgatt
     9121 cacgatagcg gcgttgcatg ttaggaatta ttccctcaaa actatgccgc ttcaccatgt
     9181 atcctccatg cggcctatga tactgaaact cgattatttc acgcccacta ccatacaaaa
     9241 cgatttgttg cattgcttca cttaaatcac aaaaaggcgt actggtatca aaaccatagt
     9301 gcctcgcaag cgactcaagc atgctgaaat aataagtggt tttcctatcc catcccctga
     9361 ttgccccttc tgccaaactg gcggttgcat cgtggactac tcttgacgga tcaaaaaact
     9421 gatccactcc aagcccatca catgaggaac acgcaccgac aggattgtta aatgaaaaaa
     9481 gccttggctc aagctcactt aaactgtaac cgcattcaga gcatgcaaac ctggaagaaa
     9541 ataaaagttc cttgaaatgg ccatccattg aagaaacgat ggcaaggcca tctgccagat
     9601 tgagtgcatt ttcaaaagat tcactcaaac gttgagccaa atcagggcgc actttaaaac
     9661 gatcaataac tacttcaata gtgtgcttgg tgcgcaatcc taatttaggg ggagaatcca
     9721 gctcataaag ctctccatca atacgtgcac gtacgtaccc ctgcgcttgc aattgttgca
     9781 aaaactggac atgctctccc ttccgttcac gaacaacagg cgccagaatc atcactttac
     9841 tgtcttctgg taaagccaat acctgatcaa ccatttggct aatggtctga gcatgcaagc
     9901 tggtgtggtg ggtaggacac cttggctctc cgactcgggc ataaagcaag cgcaaatagt
     9961 cataaatttc ggtaatggtg cctactgtgg atcggggatt atgagaggta gccttttgtt
    10021 ctatagaaat cgccggggat agcccctcta tcgaatcaac atcaggtttt tccatcatgg
    10081 acaaaaactg tcgagcataa gcagacaaag attccacgta acggcgctgg ccttcggcat
    10141 agagagtatc aaaggctaaa gacgacttcc cagaaccgga caatccggtt attacgatta
    10201 atttatctcg aggaagctcc aggtccaagt tcttgagatt atgggttctt gcgccacgaa
    10261 tggtgatggt atgcataaaa ttcgctaatt tgacttaaaa atacatgagt atatctctgc
    10321 tataataata aaacaaattg cacactatga tgcgaaatag ggattttaat atgaaatcac
    10381 taataaaaag cttgcttttc attgtggtct tctcaatcaa ctcgtctgct ctctatgctg
    10441 ccaatagtaa tacccgagtg attcaatggg ctcaacaaac ccttatggat actttatctg
    10501 tcagctatac tacaaaatct caagactttg ccactgttcg caaaaattac tcacttaatg
    10561 cctggagtgg aataaccgga tttttaggtg gtaacatgca aagcatacgc gaaaaacaac
    10621 taacgcttca tccggtttta ctgcccgaca cccaaattct atcaacaggc ctttatttgg
    10681 gtatgagtta ttggattatc agacaatccg tagccataga tgaactcaat atcaagctga
    10741 cttttacctt aactatcctg gggcgatctg tggcagcaag ctctccatac atagttcaaa
    10801 gcttaagtat attgctcaat acattataat tccgaattaa acaggaatca cctgaaatat
    10861 gggttgtctc cataaccaca tggaatcatc aacatgtaat ttacttgtaa aggtatactc
    10921 ccgctttaaa ttctgcttca atataaaagc aacaactgga ggacacaacc atgcctaagt
    10981 gtacgcattt tattttggct actgcattaa tttttgttac aagtacattt tatgccagca
    11041 atctacgggc aatagaacaa gaagtaagtg attcagtgat tactgccaaa attaccgcaa
    11101 agtttgccga aagcaggcac ttgaatccat taaaaatttc aattgacaca gaaaagggaa
    11161 tcgtgactct aagtggctat gctcctgata aacaggcatt catagatgcc ttgcagatcg
    11221 ccaaagacac gaaaggtgtc aaggcggttc atgccaaaga tctccacata aagaaagtca
    11281 ataccgcttt cacagatgcc ttcatcacag cagaagttga aactgctgtt ctaaaagcca
    11341 aaattctgga tgatgaatca ataccattag ttggaatcaa tgccagcaca acgaatggcg
    11401 aggtcacctt gtctgggcat gtaaaaagtg cgggctccat tgcagccatc attaaacgcg
    11461 taaatgagat acctggcgtc aaaaaaatcg ttaccaattt ggaaattcag gaaaatacat
    11521 gagtccatta acccaattta aattcttttt atggtttttt agccacttta aagtagcctt
    11581 gattggttat ttaaagccta agttaattca gcttaccgat aatgaaattg ttgtgcgact
    11641 gccattgaca agacgcagcc gcaatcatct gcattccatg tattttggtg ccttggcagt
    11701 tggtgcggat atagctggag gattacatgg tttttatcat gctaaacaag cgaaatgtaa
    11761 agtatccctg gcatttaaat cttttcaagc ccagtttctg cgtagacctg aatccgatgt
    11821 ttattttata tgcacagaag gggatgtagt taaaaaaatg atagaagaat caaaaaaatc
    11881 cggtgaacga attaataaac caattcatat caaagcttat atcaattatc tgacccatcc
    11941 agaagaaatt gctgatttta tccttgaact ctctctaaaa gttataaaat aacagcgttc
    12001 gattattata caaatattaa ccctacaaat gaaaattgtt ttttcaatct cattttctta
    12061 aacatcacat gattatcagt cttctaaaat cacttattat tgaattgatt acaccatttg
    12121 acaaagaagc cactcgtgtc taattttaaa tcatgatggc gctattttta ccgcaaggat
    12181 ttgcctatga ttgacctgat aatacaaact caacctgatg acgaaacttg cggaccaacc
    12241 agcttacacg ccatttattt atactacggt ttggatttgc cattaaacga aatcattcaa
    12301 ggagtggaca aatctctttc aggaggcacc ttaacaccca tgttgggaaa acatgccctt
    12361 cttcatggat ttagaacaac tgtttacatc tataatctga atatatttga cctcacctgg
    12421 tttcataatg gtgaagtcaa taattccttt ttgatggata aacttgaagc tcaaatgaaa
    12481 tacaaaaatg acccctatat taccaaagct tctctggcct atttagacta cttaaaactg
    12541 ggaggaacaa ttcgctccaa atcactgaac gtagacttgt taaaaaatta ttttaataag
    12601 aaaatcccca ttttaactgg cttaagtgct acttacctct atcgcagtgc aagagaatac
    12661 tttactccgg aaggcaaatc agtttacgat gatattcgtg gtgagccttg tgggcatttt
    12721 gttgtactgt gcggttatga cgtaaaaaaa cgccttgtaa ttgttgctga cccccatgca
    12781 gaaaacccac tgtctcataa caattattat aaagtaagca tcaatcgctt gattaactct
    12841 ataatgcttg gtgtactaac ttatgatgcg aatctactca taattgaacc taaagagtga
    12901 taaatgcaaa cagttattgt tactgatgat ctggatagct ggtctttttt aagtgagctt
    12961 gccccgatag tacatgcctt ggaatatctt agtggggatg agtatcatca aaataaatca
    13021 tttcgggtca ttaacttatg ccgatcttat aattaccaat cgattggcta ttatgtatct
    13081 ttacttgcac aagcccgtga tcacaaagca ttaccctctg tacacagtat tcaggatgtt
    13141 ttaaacgtca gtttatccaa actgatatct caggatactc atgaagaaat acaacacagt
    13201 ctccacgaca tcaaaggcaa tgaatttgtt ttaagcctct attttggcca aaatctggca
    13261 aagcgatatg caacattggc aaaacaactc catggtcttt ttcctttacc actcattcgc
    13321 ttttatctgg aaaagaaaaa gcaatggcga attaaaacag tagttccttt gtctttgtcg
    13381 gatgtaccag agcatcatct ggagtttatg cgccaatcag ctgaaaatta tttatcaaaa
    13441 aagagagttc atcaatggcg taaaaaacaa cgatttcatg atttggccat tcttgtcgac
    13501 ccctccgaac ctaatgcccc atccaataaa aaagcccttg aactttttgt ttcttgtggt
    13561 gaggaactgg gattaaacgt tgacatcata gataaaaatg aagcaaaagc aatcgctgaa
    13621 tacgacgctc tttttatcag agcaactact tccgttaatc attatactta tcgcgtgtca
    13681 cgacgcgcag cccaggagaa tttggtcgtt attgatgacc cacaatctat aattaaatgc
    13741 tccaacaaag tttatcttgc agagctctta agcagtcatc aaatcatgac gccagacacc
    13801 ttattcatca gcaaatatga taaggaatta cccaagattg aattcccctg tgttttaaaa
    13861 aaacctgaca gcgccttttc tcatggcgtg gttaaaatag atgatgaaaa atctttacaa
    13921 aaatctctta accagttttt taaaaattct gatttggtag tggttcaacc ttttataccg
    13981 actgaatttg attggcgcat cggcatcctt gataataaag cactttttgc ctgccgctat
    14041 tttatggcaa aaggtcactg gcaaatctat gattggcatt gccaggaaga aaataaagag
    14101 ggagatagtg aaacactacc ccttcatgaa gtgccagagc agattattaa aatagctctg
    14161 aaaagtacac gcttgattgg tgacggatta tatggtgtcg atataaaaag tcatggcaat
    14221 aaatactacg ttatagaagt gaacgataat cccaatattg atcatggcct tgaagataaa
    14281 atcctaggcc aaaatctgta tgagcagatc atgagtgtct tcttacaacg aattcgcagg
    14341 aaacatggtt atgtctgatt ttcctatttt ttctgtcttg gggattgaaa tagaatatat
    14401 gctggtggat aaagagagtt taaatgtcca acctaaaagc gatcttattc taagggcttt
    14461 ggcaggtcat caggttaatg aaatagaatt aggagacatt gcgataagca atgaattagt
    14521 attgcatgtc cttgagttaa aaaataatgg tcccaaacca ctacatgttc cagtagctga
    14581 acatttccag aaaaccatta gagagctcca gccccttctt gctcaacaca atttattgct
    14641 attacctact ggcgctcatc cctggatgga cccaaatact gaaacaattc gctggcctca
    14701 tggtaaccat gatatctata atcaatttaa ttcaattttc aattgccagg gacatggctg
    14761 ggcaaacttg caaagtatgc atgtcaacct gccttattcc aatgaagaag aattttgtca
    14821 attgcataac gccatacgct tgattttacc tttattaccc gccattgcgg caagtactcc
    14881 attccttgat ggccaagcta caggattact ggattcccgc ttgtactttt atagccgcaa
    14941 tcagcaacgc attccctcca tcagcggcga tattattcct gagtttatta gaacagaaag
    15001 ggaataccag gaacaaattc tcgaacccat gtatcgagac attagccctt atgatcccga
    15061 agggatatta caatatcaat ggctaaactc tcgtgccgcg attcccaagt ttgacatgaa
    15121 ggctattgaa attcgcattc ttgacagcca ggaatgtgta gaagcagaca ttgccatagc
    15181 aaaagccatc aatgccattt taaaatactg gcaaagcacc tcgaattttt atcttgataa
    15241 tccatgcgac accaagagtt taaaatacgt ttttgatgaa accataaaaa acggattaag
    15301 ttgtcatatc gataacaaaa aattactgga gcaatggcaa ttaccccttc aagaaatgtc
    15361 agtcaatacc atttggtcac tattgatcga aagaattagt catgaactgg ataggaaaca
    15421 gcaactggct ctggaactta ttttaaaaca gggaaatttg agtgaacgaa tcctcaaggc
    15481 ttgtagctcc tgtgtcaatc atgattcatt aaagcaagtg tatacgcaat tagcacattg
    15541 tttgcttcgt aatgaacaat ttaccccctg atataatgaa aaatatagcc cttctaatca
    15601 gttgtgaaca tgctgtcaat acagttccct atgaatatca aaccctgttt gaacctcata
    15661 aagacatatt agaatctcat agaggaattg attttggtgc gctagaaatt gcacaatcga
    15721 ttcataaaaa caaccattgc agtttgtttc aagccacctg tacaaggctc ttggttgatt
    15781 gcaataggag tatcaatcat ccccattgtt tttccgagat tacaaaaaat ttatcatcag
    15841 acaaaaagaa aaaaatactg gatgagttct attttccttt tcgcaatcgc gtcatggatc
    15901 acattcacca actgattagc caaggactgc aagtttggca tctgtccaca cacagtttta
    15961 ctcctgtaat gaacaacatc attcgcacga ctgacattgg ccttctatat gaccctcgga
    16021 gtaattcaga aaaaatcttg gccagacaat ggcaaaaaga aatcaaaata ctccaccccc
    16081 agttaaaagt acgtatgaat tatccttata aagggatcag tgatggattt actacatcct
    16141 tgcgtaaaaa atacagtcat catgaataca taggcattga actggaagtc aatcaaaaac
    16201 ttgtctttca caatcacagc ctgaatgcac tcaaaaatac actgccattg agcgtatcaa
    16261 acacgattaa ggcttatcaa aaatcagaga ggtaagcgac aacatttttt tcaagctgca
    16321 atttaaaaaa accaagtggc tgaattaaaa aattttttta caaaaaagat t
//
LOCUS       DASLOE010000055        16260 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.055, whole genome shotgun sequence.
ACCESSION   DASLOE010000055 DASLOE010000000
VERSION     DASLOE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16260
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.055"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            302..1147
                     /gene="hslO"
                     /locus_tag="U6309_001426"
     CDS             302..1147
                     /gene="hslO"
                     /locus_tag="U6309_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEN5504874.1"
                     /translation="MKEADTLQRFIFEHANIRGEIVHIEKTFQNIMNQRDYPPMVKNL
                     LGEALVSCLLLASSIKFEGNLSLQFQGDERLSLLLVQCDHNLNIRGFAKCAEGLEIAD
                     YATAFLQGRMVITLSQDNQTQAYQSLIPIQSTSMSENLMTYFAQSEQIATRVWLAVNE
                     DMAAGMLLQLMPGQDTIQREQFWEYAVQLGQTVTEGELLTIDNQTLLYRLYHETELRI
                     FESRSTRFQCRCNQEKMKQVITVLGEEEATDLIKEKGKIEITCDFCNQKYTFDSIDVT
                     LLFRK"
     gene            complement(1161..1658)
                     /locus_tag="U6309_001427"
     CDS             complement(1161..1658)
                     /locus_tag="U6309_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504875.1"
                     /translation="MKQLILHPTDISQWHALVNEAQAATRLILNENTESYLVFLLMRF
                     SQGTKLIESVVALDFLESMRKPRQLQVELLRDVGDKSLLFCGLFPGIAKKRHVSLSYF
                     SDMGQAAYLTVGELEENQTANLYLQLSEQFITLQQVLQAMRGEYIQFSQSSCGVLAEP
                     DLTMQ"
     gene            complement(1926..2885)
                     /locus_tag="U6309_001428"
     CDS             complement(1926..2885)
                     /locus_tag="U6309_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEN5504876.1"
                     /translation="MSFKGFKVVMLILLSTQSYASKLASSIVCDSTIENPCIVQDSKT
                     QFSPVIRYREVASIADVYGGNITGINKFHLSGSEQPSEKGWEAIAESISRKMGAETKK
                     VIVLDLRQESHGYLNGRAITLVSAYNWINLGKSNSQSTLDQENWLAGLRSRKIVNGVL
                     TVPQYVAKQYSQGKSMVVSTVKNEEYYVYKKGFDYYRIFISDHRAPLDSEVDALVALI
                     KNNPEDTWYHVHCRGGKGRTTTVFAMFDMLKNADKVSFEEIIARQASIPPFYNLMVTN
                     REIPELTPYYEQRLQFLIHFYEFARQSLMGYSGTWSEWKKLNI"
     gene            3063..4073
                     /locus_tag="U6309_001429"
     CDS             3063..4073
                     /locus_tag="U6309_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN5504877.1"
                     /translation="MKYRKLGKVGPDVSALGFGCMGLSEFYGKPARLDDAIKIIRNAH
                     DQYQVNFFDTADIYGKGKNEELVGKAIKPFRNQIILATKCGVVRTGSNDEMSVNNSSA
                     YIRTACEKSLKRLNTDHIDLYYLHRYDHQTPIEEVMEIMHDLIDEGKIGYIGLSETDA
                     DTIRAAYTAVKDKLVAVQTEYSLRVRAPATAVLGTCRELGICFVAYCPIARGFLSGKI
                     KKPNLLRTNGFDFRTNIPQFQPENFDQNLGLVNELETIGEKTGYTPAQLSLAWLLAQG
                     EDIIPIPGTSNPQHLAENMRSIDIFLTPEQMKDLENAYKNNPVAGKRLSKELMATFHL
                     KE"
     gene            4181..4993
                     /locus_tag="U6309_001430"
     CDS             4181..4993
                     /locus_tag="U6309_001430"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEN5504878.1"
                     /translation="MDYYFTKGKPQLVNMKTPVYLVTQFQQLMDLMQNQYQVSCLELM
                     QRSGKAACDFLVYRWPKVKKISIFCGRGDNGGQGYVLAQQAKKMGMIPTVWQVGHQMS
                     MSKPPQMHEEVWYEMNSCHQQGILLHTYSPDIDLGDPELIVDALFGVGLYGHVRPEIA
                     SLLQRLQQFTVPILAIEVPTGINASTGEIAGNALAATATITFLCMKLGLLINDGKIYS
                     GEIAFDDLLAPEAIYQQVKGIEKSSLLDSSTCFSKKIWYRNKTQKGWQLSIN"
     gene            5105..6931
                     /gene="typA"
                     /locus_tag="U6309_001431"
     CDS             5105..6931
                     /gene="typA"
                     /locus_tag="U6309_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEN5504879.1"
                     /translation="MIEKIRNIAIIAHVDHGKTTLVDKLLQQTGTLNERAPKVERVMD
                     SNALEKERGITILAKNTCVYWKGYQINIVDTPGHADFGGEVERILSMVDSVLLLVDAV
                     DGPMPQTRFVTQKAFARGLNPIVVINKIDRPGARPDWVMDQVFDLFDNLGANDTQLDF
                     PVVYTSALNGYAKLNLEDNATDMSALLQTIVDKVTPPCVDANGPFQMQISSLDYSSYV
                     GTIGIGRITRGQIKAKSPVKIIDKDGNMRSGRLLQLLGFKGLERVEVEEASAGDIIAI
                     TGIESLNISDTICDPNLVEALPALLVDEPTISMTFQVNDSPLAGQEGKYVTSRKIRER
                     LNTELLHNVALRVEDCDDPDKFRVSGRGELHLSILIETMRREGYELAISKPEVIIREV
                     EGEQQEPYEHLTVDVEENHQGTIMEKLGERRGEMQNMVPDGKGRVRLDYIIPTRGLIG
                     FHNEFLSCTSGTGLMYHVFDHYGPLIKGRIGKRLNGVMIANCPGTARAFALFNLQERG
                     RLFLEPQSVCYEGMIVGIHARDNDLVVNVTKEKQLTNIRASGSDENIILTPPVKLSLE
                     QALEFIDDDELVEVTPLSIRLRKKFLKEHDRKKASRTATSEE"
     gene            6938..7825
                     /locus_tag="U6309_001432"
     CDS             6938..7825
                     /locus_tag="U6309_001432"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEN5504880.1"
                     /translation="MKQKFKRAILYARQHRANQEVNESLHRLVDFLSTQDIEIFQDSD
                     TAASFELKAPVLPREKMGAKHDLIIVVGGDGSLLSASRMAIKVNTPVIGINRGRLGFL
                     TDILPQDIESHLGPVLNGQYNEEERFLLHTKIYDKENSYFEGDALNDVVLGRGSETHL
                     IEFDVYINQQLVSHYRSDGMILSTPTGSTAYALSAGGPIMHPQLNAIVLVPMFSHSLS
                     SRPLVIDGEAEIELYISKSNETDLRISCDGHESRVVKPGQKVAVKKNGNRLRLLHPLD
                     YHYYDTLRSKLGWESKHQG"
     gene            7829..9496
                     /gene="recN"
                     /locus_tag="U6309_001433"
     CDS             7829..9496
                     /gene="recN"
                     /locus_tag="U6309_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEN5504881.1"
                     /translation="MLTTLRIEHFAIVKQLELDFTQGMTTFTGETGAGKSIMIDALLL
                     ALGEKADPSVVRPGEEKCDITAGFCFDESSELAQWLAEHDISCHDGEIYLRRIIYAEG
                     RSKSYINGQPFPLQKIKELSEKLVHIHGQHQHQTLMQHATHRQQLDRYANNHLLLNDV
                     NTLYKQHQQIVQEINALQSQEQQADRIKLLQFQIDELQALDIQDGELEALHQEHQILH
                     HAREYLQLSQQINELLNSDDQPNICSGLNQIFQLIQQLPQEQAQIKNTAELINSALIQ
                     CEEAITEMQQFADKIQLDPERLQEVEARISSIHQLARKYHLDSKSLQEHEKTLQNELE
                     SLQNVESKLAHLHLQLAQINKVYESAALKLSESRKIHAKKLAQEITASIQQLGMPKGW
                     IEIEITPLEKMQAHGLDKVEYKVCTNPGMEPDSLNKIASGGELSRISLAIQMITAQKG
                     STPTLLFDEVDVGIGGSTAALVGKMLRKLGERLQVFCVTHQPQVAASAHHHFLVTKHS
                     NNNQTFTHISLLKASDKIAEIARMMGGLTITEQTLSHAKELISLSNE"
     gene            9663..9869
                     /locus_tag="U6309_001434"
     CDS             9663..9869
                     /locus_tag="U6309_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN5504882.1"
                     /translation="MSEKIRGTVKWFNESKGFGFLESGGKDYFVHFSAIQGSGFKTLA
                     EGATVMFKACNGQKGPQAEEVEVV"
     gene            complement(10208..11941)
                     /gene="ceg34"
                     /locus_tag="U6309_001435"
     CDS             complement(10208..11941)
                     /gene="ceg34"
                     /locus_tag="U6309_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg34"
                     /protein_id="HEN5504883.1"
                     /translation="MLMIMARVIVTYSPEVGGSSVLSLKESFTHVGSEVIDGDYRKMM
                     EDIPKEEFDKLYSTKEGRQRLFAHAKAKAMELLKNVDVLALSGNNSMIDPELFNQTRD
                     PDQNYDFSRTIAELALVHVATEKGMPILGVCGGHQVIAVYGGGEICDLNSDKLNTQKV
                     MNYDAIKVHKGSLIAQIIGGKQVQKEMDTARKAFNERMNQYNRLSEKLNALEKLNLVQ
                     EAGKLDALGKLEELDKSEVQLLLKLTGLNIEEELFDLRELVGEIGETNQLKTELQNIK
                     TSLNGLEDSHPLTSPHYETEFFGAHEQVVSKLAAGFQHTGTASDNESNEAAESEFGVP
                     IITTQFHPEVGAKGLPEAHFFYLRTEAEQEMNLNIFRYLDKAGDAYNKKKNLMVELKQ
                     INPKNQDVLLQPSQIKKTSKERQQEREKQGNKSSSKRQTESYFFESILSKIGSYLSAI
                     GEFIREGIGKFLRDILSSMFTYDSVSRLKKKEQKQSKSEHSKIDLIDSKSNSYSTVFH
                     DMKVKSPSEILRENPAKLWAEKDDSHEKTITNQTDMDNSNKVRITTELVQEEVGQEFD
                     ENHTSLYRNNL"
     gene            12380..13264
                     /locus_tag="U6309_001436"
     CDS             12380..13264
                     /locus_tag="U6309_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504884.1"
                     /translation="MVPPEGFCFNYETSTSNSFQSFMSIKDISTKALAEFTAMVLQLE
                     KEEIKVLTLSQRQDLPDAVFPNNWFSTHIDARGNNILIIYPLLAKNRQAEVNIKGLME
                     VLNQAQFEVHEIIDLRNDKDEILEGTGSLVLDRENHLLYASLSPRTSLNLVNKVADIL
                     GYTPIVFNSVDRQNKPVYHTNVIMSLSQCYAIICLDSIHDTQQKAMLSESFKETGKTM
                     IDISWNQAQHMCGNVLELYNKKGESLLILSEQAKRHFTHEQLRIIQQHSRLISTDIPT
                     IEAIGGGSARCMMAEIFY"
     gene            13412..14668
                     /locus_tag="U6309_001437"
     CDS             13412..14668
                     /locus_tag="U6309_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2828 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504885.1"
                     /translation="MKISELKKLVAELNKEVSPKVTCTNIINLAGNLSEIIEYFEQDE
                     HVGPELIYRIEAVICEFWKLVSLTLPYEEWQSSIQVAPWLILQQSLSKAGLLPTDFHH
                     PILYQRLKERYESFGHSELGVDQLLPLLIRCSRMTGYANKDPQSLDTYPHSPLNKQIE
                     ARRPQELAKLKDILCLLRAIFYLIHHCCTIEQLTLIPYLIYFRNPTTDEERRSELAIF
                     NWLTQKPADCLEFFKTNEDYIDTRSFRQISELAPLRPFIPTARSDFIKITNREHWIYP
                     FIQSRTNTSRSEYDLLNDAVNWLDTDFATEKDKSYHAALEFAHTVKKQANILTQREMK
                     IVHSALYVFCLDKYIKHRKADPRPRCTPFSLSGETKCQAAEKKQQEILGKPTKFGFFE
                     NLALNEGRLKTLTKTFEMPPYPLLRN"
     gene            complement(14735..>16260)
                     /locus_tag="U6309_001438"
     CDS             complement(14735..>16260)
                     /locus_tag="U6309_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein SidH"
                     /protein_id="HEN5504886.1"
                     /translation="IKNSLHNELGIYTSLFIDKISPEIHLHQSEILGNVAFDSKMGAE
                     IGSKINAITPGILLNNNPLKEAYVDLNNTLKEINTLLDEENKKTRDNPCRNEKIAKLT
                     SLKDRLSDLDSIPKENTVEFLKKMQEETRSSLKSLESNDALINIYDVLNSLKETIENG
                     SDLPEIKKDKLQMISDVQNILSNFDKNPAERLNLAVQILNDSNPEVLSKTKGNFLIGE
                     AFKGQVFTNYINTKISEQLNNELGPYGKVFLKQIMPDFIAKKSEIIKEIAIDNMETGL
                     ETQFKIHAPAIFEKNKELKAAYEQLNVHLKEVQSLIEAEEKKPKGNPCREEKIAALRS
                     HQSQLMNTQRIPDHETLRFLQEQNKSAKSFMGKLEKYDTMISVYDSLTEIREHVSNHK
                     SLSKEIKDEKIQEISKMEDMLKTTSKEPSIRLAEVKAHGLSDQCKNVLLKNSDNFLVS
                     FFKTLFSKLFNIKNENETLVSSFKQRLQNIKGPEPVATPMETPENEAPLVNANITRF"
BASE COUNT         5138 a         3151 c         2980 g         4991 t
ORIGIN      
        1 ctggtttttt ttctatttct ttaatttctt ctataccaaa caataatttg tctattgttt
       61 taaatagggg aagaagtaaa ctgttatttt tcataataac tccttacaat tttaaaaagg
      121 ggttttttaa gaatagtcca tgaaataaat ttctccagga aaattaaaaa ataattaaga
      181 tacgctggtt atggaaacta ctattcctct tgtaagattt tgttcaaaat aactgaactc
      241 agaaaaggcc tgagataaac attttatgtt agaataggtc tatttgtgtt atgagtttga
      301 aatgaaagaa gcagatacct tacagcgttt tatttttgag catgcgaata tccgtggcga
      361 aatcgttcat attgaaaaaa catttcaaaa cattatgaac caacgtgatt acccacccat
      421 ggtaaaaaat cttttaggtg aagcgttagt ctcatgttta ctcctggcaa gcagcattaa
      481 atttgaagga aatttaagtc tgcaatttca aggtgacgaa agattatccc tgttactcgt
      541 tcaatgtgat cataatttaa atataagagg ctttgcaaaa tgtgcagagg gtcttgaaat
      601 tgctgattac gcgaccgctt ttcttcaagg ccgaatggtc attacgctca gtcaagacaa
      661 ccaaactcaa gcctatcaaa gcctgatacc tattcaatcc acgtccatga gcgaaaatct
      721 gatgacttat tttgcccaat cagagcaaat tgctactcgt gtttggttag ctgtcaatga
      781 agacatggct gcaggtatgc tattacaact tatgccaggt caagatacaa ttcaaagaga
      841 acaattttgg gagtatgccg ttcagctggg acaaacagta actgaaggtg aattgctgac
      901 catagacaat caaacactac tctatcgtct ttatcatgaa acggaattaa gaatattcga
      961 aagccgatct acccgtttcc aatgccgttg taatcaggaa aaaatgaaac aggtcattac
     1021 tgtactggga gaagaagagg caacagattt aatcaaagaa aaaggaaaaa tagaaatcac
     1081 ttgcgatttt tgcaatcaaa aatacacttt tgattcaatc gacgtgactc tactatttcg
     1141 caaataaaat gagcctctgc ctactgcata gtcaaatcgg gttctgccag aacgccacaa
     1201 cttgactgac taaactggat atactctcct cgcatggctt gtaatacttg ctgcaaagta
     1261 ataaattgct cacttaattg caaatacaaa ttggctgtct gattttcctc aagctctccg
     1321 acagtcaaat aagccgcttg ccccatgtca gaaaaataac tcaaactcac atggcgtttt
     1381 ttagcgatac ctggaaataa gccacaaaac aacaagcttt tgtcccccac atcccttaat
     1441 aattcgactt gtaactggcg aggtttgcgc atggattcaa gaaaatcaag tgcaacaacg
     1501 gattcgataa gctttgtacc ttgagaaaag cgcatcagca aaaaaaccaa ataactttcc
     1561 gtgttttcat tcaatatcag tctggtagct gcttgcgctt cattaaccag cgcatgccat
     1621 tgactaatat cagtgggatg taatattagt tgtttcataa atacccccca tatttacctc
     1681 ttactattag tctagcaaac aaataccaat tgttttctat gactaatata ataattcgta
     1741 atgcattttt atgattaaat caagattttc ttatcttgga gcagataaat cccaagaaaa
     1801 ccaacactta atttaagatt gatatggtat ttgccgaaaa caattgagtg ccaagccttc
     1861 atgaaattcg cttcatttca aatgaaggct ttttaatatg atcttcttgc aaatggcact
     1921 agatgctaga tattgagctt tttccattct gaccaggtac ctgaatatcc cataagggat
     1981 tggcgagcaa attcataaaa atgaatcaaa aattgcagtc tttgctcata ataaggagtt
     2041 aactcaggga tttcacgatt cgtcaccatc aaattataaa aaggtggaat ggaggcttgg
     2101 cgtgcgatga tttcttcaaa gctaaccttg tctgcattct ttaacatatc aaacatggca
     2161 aaaacagtcg tcgtacggcc tttcccgcca cggcagtgga catgatacca tgtgtcttcc
     2221 ggattatttt ttatcagtgc gacaagcgca tccacttcag aatctaaagg agcccggtga
     2281 tcggatatga atatgcgata ataatcaaat ccttttttat atacataata ttcttcattt
     2341 tttacagtgc tcactaccat cgatttgcct tgagaatatt gtttggctac atattggggc
     2401 acagttaaaa caccgttaac aatttttctg gatcttaagc cggctaacca gttttcctgg
     2461 tccaaagtac tctgactatt cgactttccc aaatttatcc agttataggc gctaactaaa
     2521 gtaatcgctc tcccatttag ataaccatga ctctcctggc gaagatcgag cactatgact
     2581 ttttttgttt cagcccccat ttttctggaa atagactcgg caatagcctc ccaacccttt
     2641 tcgcttggtt gctcgcttcc tgacaaatga aatttattaa tccctgtgat attgccaccg
     2701 taaacatccg caattgatgc aacctctcta tatctaatga cgggtgaaaa ttgggtttta
     2761 ctatcctgaa caatacatgg attttcaata gttgaatcac aaacaatgga ggatgccaat
     2821 ttagaggcat aactttgagt tgataataat atcagcatta ccactttaaa tcctttaaag
     2881 ctcataataa atccttttta aatccctaaa gatgaaccgc agcattctat gtaaaagatt
     2941 tttattattc aagacgcgtt accatttcag aaataaaatc acaatcatta atatgttact
     3001 gccaaataat tctatacttt attcaactgt ccaaataaaa ccatgtcagt tgaggataaa
     3061 ccatgaaata tcgcaaattg ggaaaggtgg ggcctgatgt ctcagcattg ggctttgggt
     3121 gcatgggatt atcagagttt tatggcaaac ccgctcgcct tgatgatgca atcaaaatta
     3181 ttcgtaatgc gcatgaccaa taccaagtta atttctttga tacagctgat atttatggaa
     3241 aaggtaaaaa tgaagaatta gttggtaaag cgataaaacc ctttcgtaac cagattatac
     3301 ttgcaacgaa atgcggagta gtaaggacag gctctaatga tgaaatgagt gtcaataatt
     3361 catctgctta tatcagaaca gcctgtgaga aaagcctaaa acgcttaaat actgaccaca
     3421 ttgatctcta ctatttgcat cgttatgacc atcaaacacc aattgaagaa gtgatggaga
     3481 taatgcatga ccttattgat gaaggcaaaa ttggttatat tggtttatct gaaactgatg
     3541 ctgataccat tcgggcagct tatacagcag tcaaggacaa actggttgct gttcaaactg
     3601 aatattcact tcgagttcga gctccggcga cagcagtttt gggcacctgc cgtgaattgg
     3661 gtatttgttt tgttgcctac tgccccatag cacgtggttt cctcagtgga aaaattaaga
     3721 agcccaatct tttaaggaca aatggctttg atttcagaac taatattccg caattccaac
     3781 ctgaaaactt tgatcaaaat cttggcttag tcaatgaact tgagaccata ggagaaaaaa
     3841 caggctacac cccggcacaa ttatcattag cttggctatt ggctcaagga gaagacatta
     3901 tccccattcc cggaacctcg aatccacaac acttagcaga aaatatgcgc tcaattgata
     3961 tttttctaac tcctgaacaa atgaaagatt tggaaaatgc ttacaaaaat aatcctgttg
     4021 ccgggaaacg attatctaag gaactgatgg caacctttca tttaaaagaa taagctgtat
     4081 gtgaatagca taggagaatg acgctttctt aaccgcaaat caggaaactt tccaacattc
     4141 taaaaaccat gtttacaaaa gttagaaaag atttttttat atggactatt attttactaa
     4201 aggaaaacca cagctggtca acatgaaaac gcctgtttat cttgttacac aatttcaaca
     4261 actgatggac ttaatgcaaa accaatatca agtttcttgt ctcgaactta tgcagcgttc
     4321 gggcaaagca gcctgcgact ttttggtgta tcgttggccg aaagttaaaa aaatcagtat
     4381 tttttgtggt cgtggtgata acggaggaca aggctatgtt ttagctcagc aggcaaaaaa
     4441 aatggggatg atcccaacag tttggcaagt aggacaccag atgagtatgt caaaaccacc
     4501 tcaaatgcat gaagaagtat ggtacgaaat gaattcatgc catcagcaag gtattcttct
     4561 gcatacttat tcgcctgaca ttgatttagg cgatcctgaa ttgattgtcg atgccctttt
     4621 tggcgtaggg ttgtatggtc atgttcgccc ggaaatcgct tcgcttcttc aacgcctcca
     4681 acagttcact gtacccatcc tggctatcga agtgcctaca ggcatcaatg ccagcacagg
     4741 tgaaatagca ggtaatgcct tggctgcaac cgccaccatt accttcttgt gtatgaaatt
     4801 aggcttatta ataaatgatg gaaaaattta tagtggagaa atagcattcg atgatttact
     4861 ggcacctgaa gcaatttatc aacaagtcaa aggcatagaa aagtcaagct tgcttgatag
     4921 ttctacttgc ttctccaaaa aaatttggta tcgcaataaa actcaaaaag gctggcagtt
     4981 gagcatcaat taaagagtag atattcttta atgagcgatt agtgtataat gttaaatcta
     5041 tgaacaattg tcagttatta tcttaaatta ttaaatctcg ctattcccca actaaaggtt
     5101 ttttatgatt gaaaaaattc gcaatattgc cattattgcc cacgttgacc atggcaaaac
     5161 tactcttgtt gacaagctct tgcaacaaac tggaacgctc aatgagagag ctccaaaagt
     5221 tgaacgcgtg atggactcca atgcacttga aaaggaacgt ggaattacta tcttagccaa
     5281 aaacacttgc gtttattgga aaggttatca aattaatatt gtagataccc cagggcatgc
     5341 ggattttggc ggcgaagtgg aacgtattct ttctatggtt gacagcgttt tattgctggt
     5401 cgatgcagtt gacggcccca tgccacaaac ccgctttgtc acccagaaag catttgcccg
     5461 tggcttaaac cctatagtcg tgatcaataa aattgaccgt ccaggagcaa gacctgattg
     5521 ggtcatggat caggtgttcg acttgttcga taacttaggc gccaatgaca cccaactaga
     5581 cttccctgta gtttatacct ccgctttaaa tggctatgcc aaacttaatt tggaagacaa
     5641 tgcaacagat atgtctgccc tactgcaaac aatcgtagat aaagtcacac caccttgtgt
     5701 cgatgccaat ggtccgtttc aaatgcaaat cagctctctc gattattctt cttatgttgg
     5761 caccataggc attggccgga ttacacgcgg gcaaattaag gcaaaatctc ctgtaaagat
     5821 tattgataaa gacggtaata tgcgcagcgg acgactgctc cagttactgg gattcaaagg
     5881 gctggaacgc gttgaagtcg aggaagccag tgccggtgat attatcgcga taaccggaat
     5941 tgagagcctg aatatatccg acacgatttg cgaccccaac ctggtggagg cattgccagc
     6001 acttttagta gatgaaccta caatcagcat gacttttcag gttaatgact cgcctctggc
     6061 cggccaagaa ggtaaatacg tcacatcaag aaaaattcgt gaacgcttaa atactgaatt
     6121 actgcataat gtggcgcttc gcgtagagga ctgcgatgat cctgataaat tcagagtttc
     6181 tggtcgcggc gaattacact tgtcgattct tatcgaaacg atgcgccgtg aaggctatga
     6241 attggcaata agcaaaccgg aagtgattat tcgcgaggta gaaggtgaac aacaggagcc
     6301 ttatgaacac ctgacagttg atgttgagga aaaccatcaa ggtaccatta tggaaaaatt
     6361 gggtgaacgc cgaggcgaaa tgcaaaatat ggtgcctgat ggcaaaggca gagtacgttt
     6421 ggattacatc atcccgactc gcggattaat cggttttcac aatgaatttt tatcctgcac
     6481 ttccggtacc ggattaatgt atcatgtctt tgatcactac ggtccgttga ttaaaggacg
     6541 catagggaag cgtctcaatg gcgtcatgat agctaattgt cctggtactg cccgggcttt
     6601 tgccttgttc aatttacaag aacgtggaag attattttta gagcctcaat cagtctgcta
     6661 tgaaggaatg attgtaggta tccatgccag agataacgac ctggttgtta atgtcaccaa
     6721 agaaaagcaa ttaacaaaca tcagggcatc aggttcggat gaaaatatta tcctgactcc
     6781 tccggtcaaa ttatctcttg agcaagcgct ggaatttatc gatgatgatg agttagttga
     6841 ggttaccccg ttatctattc gtttacgtaa aaaatttctc aaggaacacg atagaaaaaa
     6901 agcctctcgt accgccacaa gtgaagaata acacattatg aaacaaaaat ttaaacgagc
     6961 catcttgtat gcccgtcaac acagagcgaa tcaggaagtc aatgaaagct tgcatcgact
     7021 ggttgacttt ttaagcacgc aagacataga aatctttcag gactcagata ctgcggccag
     7081 ttttgaatta aaagcccctg ttttacctag agaaaaaatg ggggcaaaac acgacttgat
     7141 tattgtcgtg ggtggtgatg gcagtttact ttctgcctca cgtatggcta taaaagttaa
     7201 tacaccggtc atagggatta acagggggag gctaggtttt ttaacggata ttttacccca
     7261 agacattgag tcccatttag gccctgtgct taatggacaa tataatgaag aagaacgctt
     7321 cttattacat actaaaatat atgataagga aaacagttat tttgaaggcg atgcactcaa
     7381 tgatgtcgta ttaggcagag gaagtgaaac gcatttgata gaatttgatg tttacatcaa
     7441 tcagcagctg gttagccatt accgttcaga tggaatgatt ttatccactc caactggctc
     7501 aacagcctat gccttgtctg caggaggacc gataatgcat cctcaactca atgccatcgt
     7561 cctggtaccc atgttttcac acagcctaag ttcccgacca ctcgttatcg atggggaagc
     7621 agaaattgaa ttgtatatca gcaaatcaaa tgaaaccgat ttgcgtatca gttgtgatgg
     7681 tcacgaatcc cgtgtcgtta aaccgggaca gaaagtagca gtcaaaaaaa acggtaatcg
     7741 attgcgtttg ttacaccctc ttgattatca ttattatgat acattacgaa gcaaacttgg
     7801 ttgggaatca aagcatcagg gataaaacat gctcacgaca ttgcgcattg aacattttgc
     7861 tattgttaaa caactggaac tcgattttac acaaggcatg accaccttta cgggcgaaac
     7921 aggcgctggc aaatccatca tgattgacgc cttgctgtta gcattaggtg aaaaagcaga
     7981 tccttctgta gtccgccccg gcgaagaaaa atgtgatatc acagccggat tttgtttcga
     8041 tgaatcatca gaactcgctc aatggttggc tgagcacgat atttcttgcc atgatggtga
     8101 aatctatcta aggcgcatta tttacgcaga aggccgctcc aaatcttata ttaatggcca
     8161 acctttccct ttacaaaaaa taaaggagtt aagtgaaaaa ctggtgcaca tccatgggca
     8221 acaccagcat caaacactca tgcaacatgc aacacataga caacaacttg atcgctatgc
     8281 caataaccac ctcttgttaa atgatgtcaa tacactctat aaacaacatc aacaaatagt
     8341 acaagaaata aatgcattac aaagtcagga gcaacaagca gaccgcatta aattattaca
     8401 atttcaaatt gatgaattac aagcgctgga tatccaggat ggtgaactgg aagccttaca
     8461 tcaagagcat caaattttgc atcatgccag agagtatctg cagttaagtc aacagatcaa
     8521 tgagctattg aatagtgatg atcaaccgaa tatttgctct ggtttaaacc agatttttca
     8581 actcatccag caactccctc aagaacaagc gcaaataaaa aacacagctg agttaattaa
     8641 ttcagccttg attcaatgtg aagaagcaat cactgaaatg cagcaattcg cagataaaat
     8701 tcagctggat ccggaaagat tgcaagaagt ggaagcccga atcagctcta ttcatcaact
     8761 agcccggaag taccatcttg atagcaaatc gcttcaggag catgaaaaaa cattacaaaa
     8821 tgaattggaa agtttgcaaa atgtagaaag caaattggca catctgcatt tgcaattggc
     8881 tcaaatcaat aaggtatatg aaagcgccgc attaaaatta agtgaatcac gcaaaattca
     8941 cgcaaaaaaa ctggctcaag aaataacggc cagcattcag cagttaggga tgccaaaagg
     9001 atggattgaa attgaaatca ctcctttaga aaaaatgcaa gctcacggat tggataaagt
     9061 ggaatacaag gtatgtacta accctgggat ggaacctgat tcattgaaca aaattgcctc
     9121 tggcggagaa ttatcccgaa tcagtcttgc aatacagatg attacagcac aaaaaggttc
     9181 aactcctacc ttattatttg atgaggtcga tgtgggtatt ggtggctcga ctgctgcact
     9241 agttggaaaa atgctgagaa aacttggaga aagattgcag gttttttgtg ttacccacca
     9301 gccgcaagtt gctgcctcgg cacaccatca ctttttagtc accaaacata gtaataacaa
     9361 tcaaactttt actcatattt cattgcttaa agcatccgat aaaattgccg agatcgctcg
     9421 catgatgggt gggctcacta taacagaaca aacactttct catgcgaaag agctgatatc
     9481 gctgagcaat gagtgaacac gtcgatcatt ttcgggtgaa attttaccaa tctattgatt
     9541 taatttccta ttttttatac aaaatctaaa ttaattcaaa aaattaaaaa aaaattaata
     9601 aaaaaacagc acaattaata aaataatgct acaatggatg cgtaacaatt taggaaatta
     9661 acatgtcaga aaaaattcgt ggtacagtca agtggtttaa tgagtccaaa ggtttcggtt
     9721 ttttagaaag tggcggcaaa gattattttg tgcattttag tgcgatccaa ggcagtggtt
     9781 ttaaaaccct ggctgaaggc gctactgtaa tgtttaaagc ttgcaatggt caaaaaggac
     9841 ctcaagctga ggaagttgaa gtagtctaat ttttttatat tagccctgtt tttttaagta
     9901 taatttaatc cttagaaaag cagggctttt tttatctaca acattcttaa aatttctgtt
     9961 ctgcacttaa aaaaactctg ctcaagcaat tgctttggca aatattggtt aaagccagct
    10021 caattatatg ataaagtttg tgccatcatt caattcaata gcgctcaaat tattattggc
    10081 aaatacctgt gctgataata gctgttacta gcattctgtt tttcaatgga ggcagcatag
    10141 tagataaata tcaatccccc atcgtttctg gtagactgca taaagacgtt cggtcatatc
    10201 ttgtctatta taaattgttt ctgtacaaac tagtatggtt ctcatcaaac tcttgtccaa
    10261 cttcttcttg aaccaattct gttgttattc ttaccttatt agagttatcc atgtcagttt
    10321 gattggtgat ggttttctca tgactatcat ctttttctgc ccacaacttt gctggatttt
    10381 ctcgtaatat ttcagaaggt gacttaactt tcatatcatg aaacacagta gaatatgagt
    10441 tacttttgct atcaatgaga tctattttcg aatgctcaga ttttgattgt ttttgttcct
    10501 tttttttcag tcttgaaacg gaatcatatg taaacatact tgataggatg tctcttaaaa
    10561 atttaccaat tccttctctt atgaattctc ctatggcact taaatatgaa ccaatttttg
    10621 agagtatact ctcaaaaaag taagattctg tttgtctttt tgatgaagac ttgttccctt
    10681 gtttttccct ctcttgctga cgttctttgc ttgttttttt aatttgagag ggttgaagca
    10741 atacatcttg atttttcgga ttgatttgtt ttagctcaac cattaggttt ttctttttat
    10801 tgtaagcatc acctgctttg tcaaggtatc taaatatatt aagattcatc tcttgttcag
    10861 cttctgttct tagataaaag aaatgagcct cgggcagtcc tttagcacca acttcgggat
    10921 ggaattgcgt ggttataata ggaacaccaa actctgattc tgctgcttca ttggactcat
    10981 tatcacttgc agtacctgta tgttgaaagc ctgctgctaa tttggaaaca acttgctcat
    11041 gcgcgccaaa aaactccgtt tcataatgcg gggaagtaag aggatgagaa tcttctaatc
    11101 catttaaact cgtttttata ttttgtagtt ctgtttttaa ttgattagtc tcgcctattt
    11161 ctcctactaa ttctcttaaa tcaaacaact cctcttctat atttaatcca gtcaacttta
    11221 ataaaagttg cacttcagat ttatctaact cttctaattt tcctaatgca tctaatttcc
    11281 ctgcttcctg tactaggttt aatttttcca atgcatttag tttttctgac aatctattat
    11341 attggttcat tctttcatta aacgcttttc tggctgtatc catttctttc tggacttgtt
    11401 ttccaccaat aatttgagct attaatgaac ccttatgcac tttgattgca tcataattca
    11461 tgactttttg tgtatttaat ttgtcagaat ttaaatcgca tatttctcca ccgccatata
    11521 ctgcaatgac ttgatgccca ccacaaacac ctagaatagg catacctttt tctgtcgcta
    11581 catgcactaa tgccaattca gcaattgttc tggaaaaatc ataattttga tcggggtctc
    11641 ttgtttgatt aaaaagctca ggatcaatca ttgaattatt accagataat gctaacacat
    11701 caacattttt aagtaattcc attgcttttg ctttggcgtg tgcaaataat ctttgtcggc
    11761 cttctttagt ggaatacaac ttatcaaatt cttctttggg tatgtcttcc atcattttcc
    11821 tgtagtcgcc gtcaataact tcggaaccga catgtgtaaa tgattctttc aacgaaagaa
    11881 cactgcttcc accaacttca ggactatagg taacaattac tcttgccatg atcataagca
    11941 tatttgaatg agcaaatgat attataaatg atcattatta agggatcctt atttattggt
    12001 ttggttttga gcaattcgca atttcaggta attatttgat gaaactgaaa attaaaagga
    12061 gttctggaca agagcaaatt atttgcagcc gtgaactatg accttcgctt tgataccatg
    12121 ggatacaata tagagcttga ttagcggcct tattcctgga acactattta ccctatttgt
    12181 tttgcctgcg atttacaaca tcatcaaatc caaactaatt gatttacaaa aacaagcggc
    12241 aaaaatcggt gcagcaaaga ataaagattt atttaaaatg caataagtgg atatggattt
    12301 ttgcaaatac tatcagtatt ctaggcaaca tgatttcccc gctgacttat ttatgacttg
    12361 taaaggatct acggtattaa tggttccacc ggaaggattt tgctttaatt atgaaacatc
    12421 aaccagcaac tctttccaat cattcatgtc tatcaaagac ataagcacta aggcgctagc
    12481 agaatttact gctatggtgt tacaactgga aaaagaagag ataaaagtat taaccctatc
    12541 gcaaagacag gatttaccgg atgccgtctt tcccaataac tggttttcaa cacatattga
    12601 tgcaagaggg aataatattc ttattattta tccattacta gctaaaaaca gacaagcaga
    12661 agtcaatatc aaaggattaa tggaagtact gaaccaagct caatttgaag ttcatgaaat
    12721 aattgactta agaaacgata aggatgaaat ccttgaagga actggcagcc tggttttaga
    12781 tagagaaaac caccttcttt atgcgtcttt atccccgaga acttccctga atctggtaaa
    12841 caaagtcgct gatatcctgg gatacactcc tattgtattt aatagtgtag atagacagaa
    12901 caagcccgtc tatcatacca acgtcataat gagtctaagc caatgctacg cgattatttg
    12961 cctggattct attcatgata ctcaacaaaa agccatgctt tcagaaagtt tcaaagaaac
    13021 aggcaaaaca atgattgaca ttagttggaa tcaagcacag cacatgtgtg gtaatgtttt
    13081 ggaactttat aacaaaaaag gtgagagcct tttaatttta tccgagcaag ctaaaagaca
    13141 ctttactcat gagcagctaa gaatcatcca acaacactcc agattaatat caacagatat
    13201 ccctacaata gaagccattg gaggtggaag cgcacgatgc atgatggctg agatttttta
    13261 ttaaaagttc aaaaaatttt gctaccagtt taatttcctt tcagatattc tctaaaacga
    13321 gacttaagaa aaaccccaag gtcaaggtaa aaaaagattg gaggtttact taaggcctgt
    13381 aaaaaactaa tcattcaggc agggaatcat catgaaaatt agtgaattaa aaaaactggt
    13441 tgctgaatta aataaagagg tttcaccaaa ggttacatgc acaaacataa ttaacctggc
    13501 tggcaattta tcagaaatca ttgagtattt tgagcaggat gaacatgttg gtccagagct
    13561 tatataccga atagaagcgg tgatttgtga attctggaag ctagttagtc tcacattacc
    13621 ttatgaagaa tggcaaagca gtattcaagt tgctccctgg cttatattac agcaatcatt
    13681 atccaaagcc gggttactgc ctacggattt ccaccatccc attttgtacc agcgcttaaa
    13741 agaacgctat gaaagctttg gtcacagcga gttaggagtt gaccagctct tgcccttgct
    13801 tatccgttgc agccgcatga ctgggtatgc caataaagat ccacaatctc ttgatactta
    13861 tcctcactca ccattaaaca aacaaatcga agcgagaaga ccacaggagc ttgccaagct
    13921 aaaagacatc ctctgccttt taagagccat cttctattta attcatcatt gttgcaccat
    13981 tgagcaattg acgttaattc cttacctgat ttattttcgt aatcccacta cagatgaaga
    14041 aagacgatct gaattggcca tttttaactg gctaacccaa aaaccagctg attgccttga
    14101 attctttaaa accaatgaag attacattga cacaagatca tttaggcaaa ttagtgagtt
    14161 ggcaccatta cgccctttta tcccaactgc gcgtagtgat tttattaaaa taactaatag
    14221 agaacactgg atataccctt ttattcaaag caggacaaat acatcaagat cagagtatga
    14281 tttgctgaat gatgcagtca attggttaga tactgatttt gcaactgaga aagataaatc
    14341 ctatcatgct gctttggagt ttgcccatac cgtcaaaaag caagcaaaca ttttaaccca
    14401 aagggaaatg aaaatagtgc attccgcgct ctatgtcttt tgtctggaca aatacataaa
    14461 acatcgtaaa gcagatcctc gtccaaggtg cactccgttt tcactttcgg gagaaacaaa
    14521 atgccaagca gccgaaaaaa aacagcagga gatactggga aaaccaacga agtttggatt
    14581 tttcgaaaat ctggccttga atgaaggaag attaaaaaca ctgaccaaga cttttgaaat
    14641 gccaccctat cctttactaa gaaattagtt aaatttaatt gttgttttga ctgaaatgaa
    14701 agattatgtc tgattaaatc aaaactaatt cgaattagaa tctggtaata ttggcattca
    14761 ctaaaggtgc ttcattttct ggagtctcca taggcgtagc gacgggctct ggacccttga
    14821 tattttgcag cctttgtttg aatgatgaaa ccaaagtttc attttcgttt ttaatattaa
    14881 ataatttgga aaatagcgtt ttaaagaaac tcaccagaaa attgtcagaa tttttaagta
    14941 atacattttt acattgatca cttaggccgt gagccttgac ctctgccaaa cgaatagaag
    15001 gctctttgct ggttgttttt agcatgtctt ccatctttga tatttcctga attttttcat
    15061 ctttgatttc ttttgacaaa ctcttatgat tactaacatg ctctcttatt tctgtcagtg
    15121 agtcatacac actgatcatc gtgtcatatt tttctaattt acccataaaa cttttcgcac
    15181 ttttattctg ctcctgcaaa aacctgagtg tctcatgatc cggaatacgt tgggtattca
    15241 ttaactgaga ctgatgggat cttaatgcgg ctattttttc ttcacggcag ggattacctt
    15301 taggtttctt ttcttctgcc tcaatcaggc tctgtacttc ttttagatgg acgtttaatt
    15361 gctcgtaagc tgcttttaac tccttattct tttcaaaaat agcaggagca tgaattttaa
    15421 actgggtttc aagccctgtt tccatattat caatggctat ttctttgatt atttcagatt
    15481 ttttggcaat aaaatcaggc atgatttgct tcaagaatac ctttccataa ggaccgagtt
    15541 cgttgttcaa ttgctcgctg attttagtat taatataatt agtaaaaact tgtcctttaa
    15601 atgcttctcc aataagaaag tttcctttag tttttgacaa gacttcgggg ttagagtcat
    15661 ttaatatttg cactgccaaa tttaaacgct cggctgggtt tttatcaaaa ttcgagagga
    15721 tattctgaac atcggaaatc atttgtaact tatctttttt gatttcgggt aagtcactcc
    15781 cgttctctat ggtttctttt aaagaattaa gtacatcata aatattgatt aaggcgtcat
    15841 tgctttccag agatttcagc gaagaccttg tttcttcttg catcttctta aggaattcca
    15901 ctgtattttc tttaggaata ctgtccaaat cactcaagcg atcctttaga gaagtcaatt
    15961 tggctatttt ttcattgcga caaggattat ccctggtctt tttattttcc tcatcaagta
    16021 atgtattaat ttccttcaat gtattattta aatcaacgta cgcctctttc aatggattgt
    16081 tattcagcaa aattccggga gtaatcgcat taatttttga gccaatttca gcccccattt
    16141 tgctgtcaaa tgcaacattt cccaatattt cagattgatg caaatgaatt tccgggctta
    16201 ttttatcgat aaataaactg gtataaattc ccaattcatt gtgcagagag tttttaatat
//
LOCUS       DASLOE010000056        16167 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.056, whole genome shotgun sequence.
ACCESSION   DASLOE010000056 DASLOE010000000
VERSION     DASLOE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16167
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.056"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            72..2237
                     /locus_tag="U6309_001439"
     CDS             72..2237
                     /locus_tag="U6309_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC26A/SulP transporter family protein"
                     /protein_id="HEN5504887.1"
                     /translation="MTRSQLILAILAGLVISIDNMISIVAFSSLIYQGALEIYIPLVI
                     NILAISVIIISASYLFMSSPPYAIAQLQDEAAILYSAMGLVIIKSMPSSSTNEAIFIT
                     ILFMLGVTTCLTGLSFYFVGKFRFGNIIRYLPYPVICGFMAATGWLVLSGTFALLTGK
                     ALHQNWLVFFHKEEILLWAPGFIAGIVVYIFKEKLGYRYALQLMFISLVILFYALFYI
                     FNLEYLVDPTEGYVLGPFNQSHIDSLMKPEIWQMIHYPFSMTLLNYIGLVILVSFIAL
                     LLNASSYELIVNKQLDLNKELRIAGIGNVFSGLLGGMVGYLSLSASTVAKEYGGARII
                     GVMAFVPMLLILCFGAVVVECFPKMAIGCYLFYIAFCFLSEWLINTWRLLNLSEYLIV
                     LLILVIAIVFNMITAVAIGTVISIFIFAFRYSKINPVKYLFSGADYNSSFERNPISQS
                     ILSTQGDEIQFLKLQGFLFFGSIYRLLQLIKSIQKARYIILDFELVYNIDSSRIILMK
                     NLKLHAEKNNISFAICSLKPIVYHELMKTNLLQSKTNWLKVFTDQESALEWCEDQVIE
                     KYTENINIEEVTLEKQLLYLGFSSELVAWISEKASIKPYNPDDIICHEGEESNSVYFI
                     HRGRVTAFVGDKRVLVVGSGNVLGEIAIYTHKKRTATLKTNEKTIVYEIDLKSIQELS
                     KNKPLLLVQFHVCMAKILANRLSVLNKRIKVFDSTAILQ"
     gene            complement(2285..3352)
                     /locus_tag="U6309_001440"
     CDS             complement(2285..3352)
                     /locus_tag="U6309_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEN5504888.1"
                     /translation="MDDLKKYSFLLVYLLIPLPFVSLYLGGWYSFLPFFIVFAFIPLI
                     DAFLHDSVNPTKEQEQELLKDKFFKYITCAYVPIQITIIAYGVYIVSTQNLVWNEWLG
                     FTMSLGLISGGVGINFAHEMMHKNSKLQQLMSKILLVTVCYGHFFIEHVKGHHVKVAT
                     PDDPATARLGENLYQFLPKTLLGSFQSALILEEKRLSHKNYSIWSIHNNFWWILTVPL
                     MICIVCFIFGGLIALSFFLLQSFIAILTLEIVNYIEHYGLERKMLSNGQYEKVNPNHS
                     WNANHWLSNQLLIHLQRHSDHHAHGARPYQILRHMENSPQLPSGYLGMIIIALIPPLW
                     HYIMDKRVLAYRIQLRQLEQL"
     gene            complement(3482..4333)
                     /gene="rsmI"
                     /locus_tag="U6309_001441"
     CDS             complement(3482..4333)
                     /gene="rsmI"
                     /locus_tag="U6309_001441"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEN5504889.1"
                     /translation="MTNTLATGLGTLYIVATPIGNREDISFRALNTLKSVDLILAEDT
                     RHSMQLLTSLGIKNNLASLHTHNEANKSNEIIELLLHGKSIALISDAGTPLISDPGFP
                     LVKQARQHHIPVVPVPGACALIAALSAAGVPCDSFAFLGFLPAKQSARKHALESSRSV
                     PYTIVFYESTHRIIDCLNDIAEIYGQDYELVLAKEITKTFERFVSGKIKEIKDWLLSE
                     PGHIKGEFVLIFPPRSTNKDPNSHEELLKILLEELPLKQAVAIACRLTNANKNQLYEE
                     ALKLKES"
     gene            4373..6184
                     /locus_tag="U6309_001442"
     CDS             4373..6184
                     /locus_tag="U6309_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEN5504890.1"
                     /translation="MLIKQLKLRMFLVLVSLFLLCQCTKTVNSTEPVVTVNKKLKSPY
                     SLPTAAYLAMAKNQQGHEKQSALISAAGRLITDGQWKQGTAILAQTGELTVEQANEKN
                     LLLAKVDIMRDRPRDALAKLARIKEPERLSLYNQIQFHEQLAKSFRSIGSYSESVSER
                     IKLESLLPDEENQVNNRRALWLTLTSLPQEELNTMALEAVNKSEMQGWLQLAVISRKY
                     RNNSKSLLAALDQWQMHFSNHPANQILPNPLDSISGKLLAPPKQVALLLPLSGPLSGP
                     GNAIREGFMAAYKANRGDESTKIKVYDTSKDDITNTYRQAISDGAEYVVGPLTKTQVA
                     TIASMEHPVPTLLLNDTDTSTQSNSYSLGLSPVNEAIQVAIKARSKGYKKALIIAPNN
                     AWGNEVTKAFINQWNEDGGHIVDSLRYEAKQDLNKSMKDFLQITNSQEREKKLKQVLG
                     YNFQSTTSRRQDFDMIFLLAYPSKARQIMPLLKYYYAGDAPVYATSSVYGGSANALKD
                     KDLDGIIFCDMPWVFSHQMGTKNWPEQFNSYNRLYALGVDSYTLATQLNQLILFPADG
                     SNDSTGILYLKPTQQVARVLEWGQFKKGLAHSLGDTV"
     gene            6206..6562
                     /locus_tag="U6309_001443"
     CDS             6206..6562
                     /locus_tag="U6309_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEN5504891.1"
                     /translation="MTQEKGKFAEQLALNYLKENGLALVMQNYHCRLGEIDLIMREGS
                     YLVFVEVRSRSNMNFGGGLASITYEKKQKIIKATSHYMIKYRIQDKFPIRFDVISIDG
                     KSNKITWLKNAFDAGC"
     gene            6592..7188
                     /locus_tag="U6309_001444"
     CDS             6592..7188
                     /locus_tag="U6309_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN5504892.1"
                     /translation="MQLEERVRHLFGVTIEAKISAADVLSGSIAKAGKLLVNCLLNDG
                     KIFICASGGSSANCMHFSSSMLNHFEVERPSLPVINLCADASCLSSFADEHHFGQVFA
                     RQIQALGQEQDVLLLLTTSGNSDSMLQALHAASERGMDTIALSGRDGGVLANHMGPED
                     IEIRVASDSTALIRELHLFILHCFCDLIEQSLFGQVLG"
     gene            7192..7764
                     /locus_tag="U6309_001445"
     CDS             7192..7764
                     /locus_tag="U6309_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEN5504893.1"
                     /translation="MNMNLKFKSIIVMMIGALLTGCVAVVVAGAAAGMVYDRRSVTTM
                     EADARLFHVIHKTIIKDPRFRHSRILVSSFNRVVLLVGQTPAASLRVLAERIAQNAPG
                     VKRVYDEITVDNPIPLTQRTKDSWITSQVRSNMLTKKGLESGSIRIVTENGVVYLMGI
                     VSDEQALLAVDVARRVAGVRKVVKIFQYIR"
     gene            7766..8353
                     /locus_tag="U6309_001446"
     CDS             7766..8353
                     /locus_tag="U6309_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEN5504894.1"
                     /translation="MFKHGCFILIVFFLASCLSGCLGVLWTGATLAYDRHNVYKKLDD
                     YNLIKALNDVLAVNRTFKNSETVLDIAVFNRDILIAGHVPTQELYDELQLRLSKVKGY
                     RRLFNRVIINKMPSNSIQDSWITTKIRSQIFADSSIDPNAFKVVTSDRVVYLMGDVQT
                     EQAEKVIKIARYTGGVQKVIKLMRYYTYQTSTNMA"
     gene            complement(8423..8668)
                     /locus_tag="U6309_001447"
     CDS             complement(8423..8668)
                     /locus_tag="U6309_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504895.1"
                     /translation="MSEKIQHTAARLQEKMSNRYSDGLSHKHNKNHLSYRQHCRSHPC
                     NQTNNNDEENIVNGSTGLLREFNLRKIRKRLDKGRFH"
     gene            8822..9217
                     /locus_tag="U6309_001448"
     CDS             8822..9217
                     /locus_tag="U6309_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEN5504896.1"
                     /translation="MNKQLNKRGITRLWLAQLGVTIILAGLCAILFGVNAASSALLGG
                     LVCIIPNAYFAIKLFKHQGARAARQIVNSFYKGEALKIILSMFLFALVFLLCRITPAA
                     FFASYILVLMTHWFAPWIIVNKQNRPKSD"
     gene            9219..10046
                     /gene="atpB"
                     /locus_tag="U6309_001449"
     CDS             9219..10046
                     /gene="atpB"
                     /locus_tag="U6309_001449"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEN5504897.1"
                     /translation="MVSSTNYIKHHLTYLTYNVNDMTFGSSGGFWTLNLDTIFFSIAL
                     GLIALAIMYLAARKVTTGVPGKLQNFAELMLEFADNQVKDCFHGKNNLIGPLALTIFM
                     WVFLMNFMDIVPVDILPVLAQLVGIHYLKVVPTNDLNLTFGLSLSVFFLIVFYSLKIK
                     GVKGFVKELTLQPFNHPLFIPFNLLLELVGLIAKPISLALRLFGNLYAGELIFILIAL
                     LTLNATTSSLISTMTLGTAQFILALAWSIFHILVITLQAFIFMVLTIVYLSLAHEDH"
     gene            10123..10362
                     /gene="atpE"
                     /locus_tag="U6309_001450"
     CDS             10123..10362
                     /gene="atpE"
                     /locus_tag="U6309_001450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEN5504898.1"
                     /translation="MTVIAVALLIGLGALGTAIGFGLLGGKFLEGSARQPEMVPMLQV
                     KMFIVAGLLDAVTMIGVGIALFFTFANPFLSSLGS"
     gene            10414..10884
                     /locus_tag="U6309_001451"
     CDS             10414..10884
                     /locus_tag="U6309_001451"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEN5504899.1"
                     /translation="MDINLTLIVQMLVFAAFVLFTMKLVWPPLAKALEERQDKIADGL
                     AAAERGRKELELAQHRVKDELKQAKAHSADIIDKANKRASEIIEAAKEAAKREAQIQA
                     KLAQEQIAQQVNHAKEELRKQVAKLAITGAEKILMREVDAKANSELLDNLIEEI"
     gene            10886..11428
                     /locus_tag="U6309_001452"
     CDS             10886..11428
                     /locus_tag="U6309_001452"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEN5504900.1"
                     /translation="MSDSTTIARPYAKAIFEHALAEKKLSEWSEYLTLLAQVVLTPQA
                     TQFIANPASTDEQQIELLIEVCGSKFKKNDALNNLIKLLTTNKRLMLLPEIEALYEVY
                     RAEQEKILEVDVVSYSELTPAQQQRLSESLSQRLSRKVSLKISIDPSLLGGALIRAGD
                     LVIDGSVRGKLNMLGTSLAA"
     gene            11446..12999
                     /locus_tag="U6309_001453"
     CDS             11446..12999
                     /locus_tag="U6309_001453"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEN5504901.1"
                     /translation="MSEQVALNPSEISELIRKKIDQFSVVSEARNEGTIVSLKDGIVR
                     LHGLADVMAGEMIEFPGGVYGLALNLERDSVGAVILGDSSTLAEGQKGKCTGRILEVP
                     VGKGLLGRVVDALGNPIDGKGPIESSGMSPIEKVAPGVITRKSVDQPVQTGLKAIDAM
                     IPVGRGQRELIIGDRQTGKTAIAIDAIINQKGTGVKCVYVAIGQKASSVASIVRKLEE
                     HGALEHTIVVVAGASDSAALQYIAPYSGCTMGEYFMERGEDALIVYDDLTKQAWAYRQ
                     ISLLLRRPPGREAYPGDIFYLHSRLLERAARINADEVEKLTNGEVKGKTGSLTALPII
                     ETQAGDVSAFVPTNVISITDGQIFLDVDLFNSGVRPAINSGLSVSRVGGAAQTKIMKK
                     LGGGTRLALAQFRELEAFSQFASDLDDATRKQLERGQRITELMKQKQYSPLTVAEMGV
                     SLFVVEKGYLDDVPVNEISSFEASLHDYMRSTHAALLHAINEAGAYDNEIEAKLKKAV
                     EEFKNTGSW"
     gene            13089..13955
                     /gene="atpG"
                     /locus_tag="U6309_001454"
     CDS             13089..13955
                     /gene="atpG"
                     /locus_tag="U6309_001454"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEN5504902.1"
                     /translation="MAGAKEIRSKISSINKTRKITRAMEMVAASKMRKTQERMRASKP
                     YANKIYEVIKHIARAASEYRHPFMSEREIKRIGIIVVTTDRGLCGGLNSNLFRETIRT
                     IRNWQEHGKEVDIAVIGRKGQAFFRRVGGNILGSIDHLGDTPSINDFIGVVKIMLDAY
                     YNGTIDSLHIVYNEFINTMTQKPFVKQLLPLPKSEEDKKTLGHHWDYIYEPEAKELLD
                     EILERYIELQVYQAVVENIACEQAAKMIAMKSATDNAGDLIKEFQLAYNKARQAAITQ
                     ELAEIVGGAAAL"
     gene            13968..15344
                     /gene="atpD"
                     /locus_tag="U6309_001455"
     CDS             13968..15344
                     /gene="atpD"
                     /locus_tag="U6309_001455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEN5504903.1"
                     /translation="MSLGTVVEVIGAVVDVEFPRDSVPKVNDALKLVDSDLVFEVQQQ
                     LGDGVVRTIAMGTTDGLKRGLKAENTGHPIQVPVGKKTLGRIMDVLGRPVDDAGPIDA
                     EETWAIHRKAPSYEEQAGSQELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LIRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRV
                     ALTGLTMAEKFRDEGRDVLLFIDNIYRYTLAGVEVSALLGRMPSAVGYQPTLAEEMGM
                     LQERITSTKTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRQIAELGIYPAVD
                     PLDSTSRQLDPLIVGQEHYDTARRVQQTLQRYKELKDIIAILGMDELSEEDKRVVTRA
                     RKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIKGFQGILAGEYDDLPEQAFYMVGSIEE
                     AVAKAKTL"
     gene            15356..15778
                     /locus_tag="U6309_001456"
     CDS             15356..15778
                     /locus_tag="U6309_001456"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEN5504904.1"
                     /translation="MSITTHLDIVSAEHEIFSGVVEMVVATGELGEIGITPGHAPLLT
                     VLRPGEVRITLQGGTQDIYYVQGGMLEVQPHCVTILADVAERAEHLDEAAALAAKAKA
                     EAAIASKGGDIDYSVAAAELARAVAQIRAIQKTRKKMK"
BASE COUNT         4952 a         2782 c         3475 g         4958 t
ORIGIN      
        1 tgaaaaactg gatttttctg ttatttacta aaataatcct ttggttttga aatgaatgag
       61 tgagaggtag gatgactcga agtcaattga ttttggctat tttagcaggc ttggtcataa
      121 gtattgacaa tatgattagt attgttgcat tttcttcgct tatttatcaa ggtgcattag
      181 agatttatat tccacttgtc attaatatat tggcaatttc agtaattatt ataagcgcca
      241 gctatttgtt catgtcttcc cctccttatg caatcgctca gttgcaagat gaagcggcaa
      301 ttttgtattc agccatgggt ttagtgataa ttaagtcgat gccatcaagt tctacgaatg
      361 aagccatctt tattacgatt ctctttatgc ttggagtcac tacctgttta acgggcttat
      421 cattctactt cgttggaaaa tttaggtttg gaaatattat ccgttatctg ccatatcctg
      481 tgatttgtgg ttttatggca gcgacaggct ggctagtttt atcaggaaca tttgcattat
      541 taactggtaa agcgctgcat cagaattggc ttgtcttttt tcataaagag gaaatattat
      601 tgtgggcgcc aggttttatt gcgggaatag ttgtttatat ttttaaagaa aaattaggtt
      661 atcgatacgc gcttcaattg atgtttattt ctcttgtaat tctattctat gctctttttt
      721 atatttttaa tttggagtat ttggtcgatc caaccgaagg gtacgtcttg ggacctttca
      781 atcaaagcca tattgattcg ctcatgaaac ctgaaatatg gcaaatgatt cattatccat
      841 tctcaatgac cctattgaat tacatagggt tagttatact ggtaagtttt attgccttgt
      901 tattaaacgc aagcagttat gaattgatag ttaataagca attagattta aacaaggaac
      961 ttcgtatagc aggtattggc aatgtattca gtggattact tggaggaatg gttggctatt
     1021 tgtctttgtc agcgtccact gttgcaaaag agtatggcgg cgctcgtatt ataggggtga
     1081 tggctttcgt tcccatgttg cttattttgt gctttggtgc tgtggtcgtt gaatgtttcc
     1141 cgaaaatggc tatcggctgt tatttgtttt atatcgcttt ttgctttcta tctgaatggt
     1201 tgatcaatac ctggcgttta ttaaatttaa gtgaatattt aatcgttttg ctgatattgg
     1261 ttattgctat tgtctttaat atgattacgg cggttgctat aggaacggtt atttcaattt
     1321 ttatcttcgc ttttcgctac agcaaaataa atccggttaa gtatttgttt tccggagcag
     1381 attataattc ttcttttgag agaaatccta tatctcaatc cattttatcg acacagggag
     1441 atgaaattca gtttttgaag ttgcaaggat ttcttttttt tggctctatt tacaggctac
     1501 ttcagttaat aaaaagtata cagaaagctc gatatattat tttagacttt gagcttgtct
     1561 acaatatcga ctcatcccgc attattttaa tgaaaaactt aaagctccat gcggaaaaga
     1621 ataatatttc ctttgcaatt tgttcattaa agcccattgt ttatcatgaa ttaatgaaaa
     1681 ccaatttatt acaatcgaaa accaactggc ttaaagtctt tactgatcaa gaatcagctt
     1741 tagagtggtg tgaagatcaa gttatagaaa aatatacaga aaatataaat attgaagaag
     1801 taacacttga gaaacagtta ctctatttag gtttttcctc tgaactggta gcatggatta
     1861 gtgaaaaagc ttctataaaa ccttataacc ctgatgatat catttgtcat gaaggagaag
     1921 agtcaaacag tgtttacttt attcatcggg gaagagttac agcatttgtc ggtgataaac
     1981 gtgtacttgt tgtgggttca ggtaatgtac ttggtgaaat tgctatatac actcataaga
     2041 agcgcacagc aacattaaaa accaatgaaa aaaccattgt ttatgaaatt gatttgaaat
     2101 caatccagga gctttctaaa aataaacccc tactcttggt tcaatttcat gtgtgtatgg
     2161 ctaaaatttt ggcaaaccgt ctctcggtac taaataaacg gataaaagtt tttgattcca
     2221 ccgccatatt gcaataaaag ttaataaaat attcttttac tgtgaaataa taaggaacaa
     2281 cgttttaaag ttgttccaat tgtcttaact gaattcgata ggcaagaact ctcttgtcca
     2341 tgatgtaatg ccagagcggt ggaattaaag ctatgataat cattccgagg taccctgagg
     2401 gaagttgagg gctgttctcc atatgacgta aaatttgata gggtcgtgca ccatgggcat
     2461 ggtgatctga atggcgttgt aaatgaatta gcagctgatt acttagccaa tgattagcat
     2521 tccatgaatg atttggattt accttttcat attggccgtt actcagcatt ttcctttcca
     2581 gcccatagtg ctcaatataa ttaacaattt ctaatgtcag gatggcaatg aatgattgta
     2641 ataagaaaaa tgacagagcg attaatcccc caaaaatgaa acagactata caaatcatta
     2701 atggcacagt aaggatccac caaaaattat tatgtatgct ccagatggaa taatttttat
     2761 gcgataaacg cttttcttcc aaaattaagg cagattgaaa cgaacctaat aatgtttttg
     2821 gtaaaaattg atataaattt tcgcctaatc gtgctgtggc aggatcgtca ggcgttgcta
     2881 ctttaacatg atggcctttg acatgctcga taaaaaaatg accatagcaa actgtaacca
     2941 ataaaatttt actcattagt tgttgcaatt tactgttttt atgcatcatt tcatgagcaa
     3001 aattgatgcc tactccacct gagataagtc ctagtgacat ggtaaatcct aaccattcat
     3061 tccaaaccaa attttgagta ctaactatat agactccata agcaatgatg gttatttgta
     3121 taggcacata ggcgcaagta atatatttaa agaacttatc ttttaggagt tcttgttcct
     3181 gctcttttgt tggatttacc gaatcatgaa gaaaggcatc aattaatgga ataaaagcaa
     3241 aaacaataaa aaagggtaga aaactgtacc agcctcctaa atataaagag acaaagggta
     3301 atgggataag taaatagacc agtaaaaaac tatatttttt taaatcatcc attttataat
     3361 tccaagcata gcctaatata gaaacaatat agcttaaaag gcgatatctt tgcttggtct
     3421 tagaggacaa aaaaaggtca tttacggaca atgttatgga aataagcgat caatattctt
     3481 tttagctctc ttttaatttc aatgcctctt catagagctg atttttattg gcattagtca
     3541 atctgcatgc aatagccaca gcctgcttta aaggcaactc ttccaacaat atcttgagta
     3601 gttcttcatg cgagttgggg tctttatttg ttgagcgagg tggaaaaatc aaaacaaatt
     3661 cacctttgat atgaccgggt tcactgagta gccagtcttt aatttctttt atttttccag
     3721 atacaaatcg ttcaaaggtt ttggttattt cttttgctaa aacaagttca taatcttggc
     3781 catagatctc tgcaatatcg ttaagacagt ctatgattct gtgagttgat tcatagaata
     3841 ctatggtata aggcactgac cgcgatgatt ccagggcatg ttttctggca ctttgtttgg
     3901 ccggaagaaa acctaaaaag gcaaaagaat cgcagggtac cccggcagca cttaacgcag
     3961 caattaaagc gcatgcgcca ggaacaggca caacagggat atggtgctgg cgagcctgtt
     4021 tcactaaagg aaatccagga tcgctaatca agggcgtgcc tgcatcactg attagtgcga
     4081 ttgattttcc atggagcagc agctcaatta tctcattgct tttgttcgct tcattgtggg
     4141 tgtgcaatga agctaaatta ttttttatcc ctagcgaagt caataattgc atggaatggc
     4201 gtgtgtcttc agcaagtatt aaatcaacgc ttttcaaggt attaagagcc ctgaaagaaa
     4261 tatcttcgcg atttcctatt ggggtagcaa ctatatagag agttcctaaa cctgttgcta
     4321 gagtgtttgt catagtttat cctcttgcga aaagcaaatt tggcgaatta acatgttaat
     4381 taaacaatta aaacttcgca tgttcttagt attggtatct ctttttttgc tttgccaatg
     4441 cacaaaaaca gttaactcaa ccgagcctgt agtaacagtt aataaaaaat taaaatctcc
     4501 atattcatta cctactgcag catacttggc tatggctaag aatcaacaag gccatgaaaa
     4561 gcaaagcgca ttgatttccg cagctggccg tttaattact gatggtcaat ggaagcaagg
     4621 aactgccatt ttagcacaaa ctggggaatt aacagttgag caagctaatg aaaagaatct
     4681 tttactggca aaagtcgaca ttatgcgtga taggcccaga gatgccttag ccaagttagc
     4741 tagaattaaa gaacctgaaa gactctcgtt atataatcaa atccaatttc acgaacaatt
     4801 ggcgaagtca tttcggtcca ttggtagtta ctctgaatca gttagtgagc gtatcaagtt
     4861 agaatcattg ctccctgatg aggaaaatca ggtcaataat cggcgggctt tgtggttgac
     4921 cttaaccagt ttacctcaag aggagctgaa tacaatggct ctggaggctg tgaacaaatc
     4981 agaaatgcaa ggctggttgc agctggctgt tatttcccgt aagtatagaa ataactcaaa
     5041 gtcgttgctg gctgccttgg atcaatggca gatgcatttt agtaatcatc cagcaaatca
     5101 gattttgcct aatcccttag acagcatatc aggtaaatta ttagctccac ctaaacaggt
     5161 tgctttgtta ttgcctttaa gtggtccatt atccgggccg ggtaatgcta tacgggaagg
     5221 ctttatggca gcctataagg ccaacagggg agatgaatca acaaaaatta aagtttatga
     5281 tacaagcaaa gacgatataa cgaacacata tcgccaagct atttctgacg gtgccgaata
     5341 tgttgttggt cctttaacga aaactcaagt cgctaccatt gcatcaatgg agcatccggt
     5401 tcctacccta ttgctaaacg atacggatac cagcacacaa agcaattctt attccttagg
     5461 cttatcccct gttaatgaag cgattcaggt tgcaataaaa gcaagaagca aaggatataa
     5521 aaaagcgctg attattgccc ccaataatgc ttggggtaat gaagtaacga aagcttttat
     5581 caatcagtgg aatgaagatg ggggccatat tgttgactct cttcgctacg aagccaaaca
     5641 agatttgaat aagagcatga aagatttttt acaaatcacc aatagccagg agcgcgagaa
     5701 aaaactaaag caggtattag gctataattt ccaatcgact acgagccgaa gacaggattt
     5761 tgatatgata tttttattgg cttatccttc caaggcccgc caaattatgc ctttattaaa
     5821 atattattat gccggagatg ctcctgtgta cgctacttca agtgtttatg gcggtagcgc
     5881 caatgctttg aaggataagg atttggatgg gatcattttc tgcgatatgc cttgggtatt
     5941 ttctcatcag atgggaacga aaaactggcc tgaacaattc aatagctata acaggcttta
     6001 tgctttaggc gtggacagtt ataccttggc tacccaatta aaccaactga tattatttcc
     6061 agccgatggc tctaacgatt caacaggtat tttatattta aagccgactc aacaagtagc
     6121 cagggttctt gaatgggggc aatttaaaaa gggtttagcc cattcactag gggatactgt
     6181 ttagttttga agtgagccaa gaataatgac ccaagaaaaa ggaaagtttg ctgaacaatt
     6241 ggctttaaat tacttaaagg aaaatggtct tgcattagta atgcaaaact atcattgccg
     6301 tttaggagaa attgatttaa ttatgcgtga gggatcttat ctggtttttg ttgaggtccg
     6361 ttcgcgttca aatatgaact ttggtggggg gcttgccagc attacttacg aaaagaaaca
     6421 aaaaataata aaagcaactt ctcattatat gattaagtat cgcatacaag ataaatttcc
     6481 tatacggttt gatgtgatta gtatagatgg aaaatcaaac aagattacct ggttaaagaa
     6541 cgcatttgat gcaggatgct aggaagataa tgaatagaag gttttgaaat gattcagtta
     6601 gaagaaagag taaggcattt atttggtgtc acaatagaag caaaaatatc agcagctgat
     6661 gtgttgtcgg gttcaatagc taaagcaggg aagttgttgg ttaactgttt attgaatgat
     6721 ggaaaaatat tcatttgcgc cagtggggga tctagtgcta attgcatgca tttttccagt
     6781 tccatgctaa atcattttga agtggaaaga ccttctttgc cggtcattaa tttatgcgct
     6841 gatgcgtcct gtttgagttc atttgccgat gagcatcact ttggtcaagt gtttgcaagg
     6901 caaattcaag ccttgggcca agagcaagat gtattacttt tactaactac atcaggtaat
     6961 tctgatagca tgctgcaagc tcttcacgca gcaagtgaac gcggcatgga taccattgca
     7021 ctaagtggcc gcgatggagg tgttttggcg aatcatatgg ggccagagga tatagagata
     7081 agagtggcat ctgacagtac tgcattgata agagagctac acttgttcat attgcattgt
     7141 ttttgtgatt taatcgaaca atctttattt ggccaagttt taggataaaa aatgaacatg
     7201 aatctaaaat ttaaatccat catagtaatg atgattggtg ctttactgac tggttgcgtt
     7261 gctgtggttg ttgcaggtgc tgcagcagga atggtttatg atagaagaag cgtaactacc
     7321 atggaggcgg acgcccgatt atttcatgtg attcataaaa ccatcatcaa ggaccctcga
     7381 tttcggcact cgagaattct tgtttccagt tttaaccggg tggtattgct ggttggacaa
     7441 actcccgctg catcattacg cgttctagca gagcggattg cccaaaatgc ccctggagtt
     7501 aaaagagtgt atgatgaaat aaccgttgat aatcccattc ccttgactca aaggacaaaa
     7561 gacagttgga ttacaagtca agtgagaagt aacatgttga ctaaaaaggg acttgaatcg
     7621 ggttcaatac ggatagtcac tgaaaatgga gtagtctatc tgatgggaat tgtttctgac
     7681 gagcaagcat tgctagctgt tgatgtggca agacgtgttg ctggagtgag aaaagtcgta
     7741 aaaatattcc aatatatacg ttagcatgtt taaacacgga tgttttatac taatagtctt
     7801 ttttttagcc agctgcttgt caggctgtct gggggtttta tggactgggg caactctagc
     7861 ctatgaccgg cacaatgtat ataaaaaatt agatgattat aatttaataa aagctcttaa
     7921 tgatgtttta gctgtcaacc ggacatttaa aaactcagag acagtactgg atattgctgt
     7981 gttcaacaga gatattttaa tagcagggca tgttccaacc caggagttgt acgatgaatt
     8041 acaactacgt ctaagcaaag ttaaaggata caggcggtta ttcaatcgtg taattatcaa
     8101 taaaatgcca tccaactcga tccaggacag ctggattacc acgaaaatcc gtagccaaat
     8161 ttttgcagac tcttcaatcg atcctaacgc tttcaaggtg gttacttctg atcgtgttgt
     8221 ctatttgatg ggagatgttc agacagaaca agctgaaaaa gtgattaaaa ttgcaagata
     8281 cacaggtggt gtacagaaag taataaaact tatgcggtat tatacttatc aaaccagtac
     8341 gaatatggcc tgatcagaat caggccaata gctgcacgtc caatccagga agtggctaac
     8401 ctgggaggat aagtatactg tatcaatgga atcgaccttt atccagtcgt tttcgtattt
     8461 ttctcaaatt aaactcacgt aacagccctg tagagccatt tacaatattt tcttcgtcgt
     8521 tattgttcgt ttgattgcat ggatgagatc tgcaatgttg tcgatatgaa aggtgatttt
     8581 tattatgctt gtgtgaaagc ccatcgctat aacgattgct cattttttct tgcagcctgg
     8641 ctgctgtatg ctgaattttc tctgacattt tttaatcctt aggcgaggtt aaatagtaaa
     8701 tcgcttaaca tgtgattaat gctaaaccct acattacaat tatagctcgt aatttgaata
     8761 tcaacgttga ttattacaat tgtttaattt ataattcgcg ccgtgaaagt taggtgaata
     8821 tgtgaacaaa cagctgaata agcgcggaat cacgcgattg tggctggcgc agttgggagt
     8881 tactataatc ctggctggcc tttgcgcaat tttatttggt gtcaatgcgg ccagttctgc
     8941 attgcttggt ggactggttt gtattatacc aaatgcttac tttgctatta aactgtttaa
     9001 gcatcaaggc gcgagagctg cgaggcaaat agttaatagt ttttataagg gtgaagcgtt
     9061 aaaaataata ttgtccatgt tcctgtttgc attagtattt ttattatgta ggatcacacc
     9121 ggcagcgttt tttgcatcat acattttggt tcttatgacg cattggtttg ccccatggat
     9181 tattgtcaat aaacagaata ggccaaaaag tgactgaaat ggtatctagc acaaactata
     9241 ttaagcatca tttaacgtac cttacctata acgtaaatga tatgacattc ggttcaagtg
     9301 gtggtttttg gactttgaac ctggatacaa tcttcttttc tatagccttg ggtctaattg
     9361 ctctggcaat tatgtattta gcggcaagga aagtaaccac tggtgttcct ggaaagttac
     9421 aaaattttgc tgagctcatg ctcgagtttg ctgataatca agttaaagat tgttttcatg
     9481 gaaaaaataa cctaataggc ccattggcat taactatctt catgtgggta ttcctgatga
     9541 attttatgga tatcgtgccg gtagatatcc ttcctgtact tgcgcagcta gtcggtatac
     9601 actacctgaa agttgtacca actaatgact tgaatttaac atttggattg tcactttctg
     9661 tattttttct aatagttttt tacagtttaa aaataaaagg cgttaaaggt tttgttaaag
     9721 agctcaccct gcagcccttc aatcacccac tattcattcc atttaattta ttgttggagt
     9781 tggttgggct gatagctaaa cccatttcct tggcattacg actttttggt aacttatacg
     9841 ctggcgaatt aatatttatt ttgatagcct tactaacctt aaatgcaaca acgtcatcat
     9901 taattagtac tatgacactg ggtacggcgc aatttattct tgcactcgcc tggtccattt
     9961 tccatatatt agtgataacc ttacaagcat ttatttttat ggtactgact atagtttatt
    10021 tgagtttggc ccatgaagat cattaaccga tttaattatc atcatccatt gtaaagggga
    10081 taaaatatgc aagctgctga attaatagca caagttcaaa gtatgaccgt cattgcggtt
    10141 gccttgttga taggtttagg tgcgttaggc acagccatag gtttcggatt gctaggcggt
    10201 aaatttctcg aaggctcagc tcgtcaacca gaaatggtac ctatgttaca agttaaaatg
    10261 tttattgtag ctggtttgct tgatgcggta accatgattg gggtaggtat tgcattattc
    10321 tttacctttg ctaacccttt cctaagcagc ctgggttctt gataacacac aaatagggcg
    10381 ctatgcgccc tcactgttac aggagaatat actttggata ttaatttaac attaatagtt
    10441 caaatgttgg tttttgccgc gtttgtacta ttcactatga aattagtttg gcctccacta
    10501 gcaaaagcac ttgaagaaag acaagataaa attgctgatg gattggctgc tgctgaaaga
    10561 gggcgcaaag agttggagtt ggctcagcat cgtgtaaaag atgagttaaa acaagccaag
    10621 gctcactctg ctgatattat tgataaagcg aataagcgag catcagagat tattgaagca
    10681 gcaaaagagg ccgcaaaacg cgaagctcaa attcaagcaa aactcgcgca agaacaaatt
    10741 gctcaacaag taaatcacgc caaagaagaa ctgagaaaac aagttgcaaa gctggctatt
    10801 actggggcag agaaaatttt aatgcgagag gtagatgcaa aagcgaatag cgaactgtta
    10861 gataacctaa ttgaagagat ttaaaatgtc tgatagtacc actatagcca gaccctatgc
    10921 aaaagcgata tttgaacatg ctttagcaga aaaaaaactg agtgaatggt cagaatatct
    10981 tacattactg gctcaagttg ttttaactcc tcaagctact cagtttatcg ctaatcctgc
    11041 atcaacagat gaacaacaaa ttgaacttct tattgaggta tgtggttcaa agttcaagaa
    11101 aaatgatgca ctaaataatt taatcaagct attaacaacc aataaaagat tgatgttgtt
    11161 gccagaaatt gaagctcttt atgaagtata tagagcagag caagaaaaaa tactggaggt
    11221 tgatgtagtc agctattccg aattgactcc agcgcaacaa cagcgtttga gcgaatcctt
    11281 aagtcaaaga ttatcacgta aggtttcttt aaaaataagt attgatccct ccttgcttgg
    11341 cggagcgcta attcgagcag gcgatttagt tatcgatggt tcagttcggg gcaagcttaa
    11401 tatgcttggc accagcttgg ccgcttaatt tagaggatag tttccatgtc agaacaagta
    11461 gcgttaaatc cttctgaaat cagtgaatta atcagaaaga aaatagatca atttagtgtg
    11521 gtttctgaag cacgaaatga aggtactatc gttagtttga aagacggtat tgtacgactg
    11581 catggtcttg ccgatgttat ggcaggtgaa atgatagagt ttccaggagg ggtctacgga
    11641 ttggctctta acctggagag agattctgtt ggtgctgtaa tattaggcga ctcatccaca
    11701 ttagctgaag ggcaaaaagg taaatgtaca ggtcgtattc tggaagtccc agttggaaaa
    11761 ggtcttttgg gacgtgtcgt tgatgcttta ggaaatccta tagacgggaa aggcccgata
    11821 gagtcatcag gtatgtctcc aatagaaaaa gtcgctcctg gtgtaattac acgtaaatca
    11881 gtagatcaac ctgttcagac aggattaaaa gcaatcgatg cgatgatccc cgttggaaga
    11941 ggtcagcgag agcttattat tggtgaccgc caaactggta aaactgccat tgctattgat
    12001 gcgattatca atcaaaaagg taccggcgtt aaatgcgtat atgtagccat tggtcaaaag
    12061 gcttcatccg ttgcatcgat tgttagaaaa ttggaagagc atggcgctct tgagcatact
    12121 attgttgtgg tagcgggagc ctctgattca gcagcactcc aatatatagc accttactca
    12181 ggttgtacca tgggtgagta tttcatggag cgtggggaag atgcattgat tgtttacgat
    12241 gatttaacta aacaagcatg ggcatatagg cagatctcct tgttattacg tcggccacca
    12301 ggtcgcgaag cttatcctgg tgatattttc tatttgcatt ctcgcttgtt ggaaagagcc
    12361 gcaagaatta atgccgatga agtagaaaaa ttaactaatg gagaagtgaa gggtaaaaca
    12421 ggatcactga ctgcattgcc gattatcgaa acacaagcgg gagatgtgtc agccttcgta
    12481 ccaacaaacg taatctctat taccgatggg caaatattcc ttgatgttga cttatttaac
    12541 tcaggtgtta gaccagcaat caattccgga ctgtccgttt cacgggttgg tggtgctgca
    12601 caaacgaaaa tcatgaaaaa gcttggtggt ggtactcggc ttgctctggc acaattccgt
    12661 gagttagaag cattctccca atttgcatct gatcttgatg atgcaacacg taagcagctg
    12721 gagcgtggac aacgcattac agagctgatg aaacaaaagc aatactcccc acttacagtg
    12781 gctgaaatgg gggtaagctt gtttgtggta gaaaagggat acttggacga tgtacctgta
    12841 aatgaaatca gctcgtttga agcttccttg cacgattaca tgcgcagtac ccatgccgct
    12901 ttattacatg caattaatga agccggtgct tatgataatg aaatcgaagc aaaactgaaa
    12961 aaagcagtag aagagttcaa gaacacaggc agttggtaag gtatggcctc aaagagctaa
    13021 ctctttgagg cacaaaggcc attatttgaa agtaatggcc tctaaaacta attaaggcga
    13081 attgaaagat ggctggagca aaagaaatac gttcaaaaat ttcgagtatt aataaaactc
    13141 gaaaaattac tcgagctatg gaaatggtgg ctgcaagcaa aatgcgcaaa acgcaagaaa
    13201 gaatgcgcgc ttctaaacct tatgcgaata aaatctatga agtcataaag catattgctc
    13261 gagcagcatc tgagtacaga cacccattta tgagtgagcg cgaaatcaag cgtataggta
    13321 ttattgttgt caccacagat agaggtcttt gtggtggttt aaactcgaat ctatttcgtg
    13381 aaacaattcg tactattcgt aattggcaag aacatggcaa ggaagttgat atcgctgtta
    13441 tcggaagaaa gggccaggcc ttttttaggc gtgtgggtgg gaacatatta ggttccatag
    13501 atcatctggg tgatacaccg agtatcaatg atttcattgg tgttgtaaaa ataatgcttg
    13561 atgcttatta caatggcacc attgattctt tgcacattgt atacaatgag ttcattaata
    13621 caatgacgca aaaacctttt gtaaaacaat tattgccgtt accaaaatca gaagaagata
    13681 aaaaaacatt gggtcatcat tgggactata tttacgagcc cgaagctaaa gaattattag
    13741 atgaaatttt agagcgatat attgaattac aagtgtatca agctgttgta gaaaacattg
    13801 cttgtgaaca agctgcaaag atgatagcaa tgaaaagtgc aacggataat gctggtgatt
    13861 tgattaaaga atttcaattg gcttataaca aagcccgaca agcagccatt actcaagagt
    13921 tggcagaaat tgtcggtggc gcagccgctt tataaagagg gtatataatg agcttaggaa
    13981 ctgtggtaga agtaattggt gcggtagttg acgtagaatt tcctcgtgat agcgtgccta
    14041 aagtcaatga tgcgttaaag cttgttgata gtgatctggt ttttgaagtg cagcagcaac
    14101 ttggagacgg agttgtgcgt actattgcca tgggaacaac cgatggttta aagcgaggat
    14161 taaaagcaga aaatacaggc catcctattc aagtgccagt aggtaagaaa actttgggac
    14221 gcattatgga tgttcttggg cgtcctgtag atgatgctgg gcctatcgat gctgaagaga
    14281 cttgggctat tcatcgtaaa gcaccaagtt atgaagagca agctggcagc caggaattat
    14341 tggaaactgg tattaaagta attgatttgc tttgcccttt tgccaaggga ggtaaagttg
    14401 gtctattcgg tggtgccggt gtaggcaaaa ccgttaacat gatggaatta atacgaaaca
    14461 ttgcaattga gcatagcggt tattcagtgt ttgcaggggt tggtgaacgt acccgtgaag
    14521 gaaacgactt ctatcatgag atgaaagact ctaatgtatt ggataaagta tcgcttgttt
    14581 atggtcagat gaatgagccg ccaggaaacc gtttgcgtgt tgctctaacc ggtttgacta
    14641 tggctgaaaa attccgggat gaagggcgag acgttctttt gtttatcgat aatatttatc
    14701 gttatacctt ggctggggtt gaagtatctg cgctgttagg ccgtatgcct tctgcagtag
    14761 gatatcagcc gacattagca gaggaaatgg gtatgctgca agagcgcatt acctccacaa
    14821 aaacaggttc tattacttcc atacaagctg tatatgtacc tgcagacgac ttgactgacc
    14881 catcaccagc tacaaccttt gcacacttgg atgccactgt agtattgtct cgtcaaattg
    14941 ctgaattagg tatttatcct gcggttgatc cgcttgattc tacatcacgt cagctggatc
    15001 cattgattgt aggtcaagag cattacgata ccgcacgtcg tgtacagcaa accttgcaac
    15061 gatataaaga attaaaagat attattgcaa ttttgggtat ggatgaactg tctgaggaag
    15121 acaaacgagt tgtaactcga gctcgtaaaa tccaaagatt cttatctcag ccatttttcg
    15181 ttgcggaagt atttacaggt tctcctggta aatacgtatc gcttaaagat accatcaaag
    15241 gtttccaagg cattcttgct ggtgaatatg atgatttgcc tgaacaggca ttttatatgg
    15301 ttggaagcat cgaggaagct gttgcaaaag ctaaaacctt atgaggcaag ataatatgag
    15361 tattacaacg cacttggata ttgttagtgc agagcatgaa attttctctg gtgtggtaga
    15421 aatggtagtt gcaactggag agttgggtga aattggaatt actccagggc atgctccatt
    15481 actaactgtg ctaaggccgg gtgaagttag aattactttg caaggtggta cacaggacat
    15541 atactatgtg cagggtggaa tgcttgaggt tcagcctcac tgtgtaacaa ttcttgctga
    15601 tgtggcagaa cgagccgagc acttggatga agctgctgca ttggcagcta aagcgaaagc
    15661 tgaagccgct atagccagta aaggaggcga tattgattac tctgttgctg cagcagaatt
    15721 ggccagggct gtcgctcaaa taagagcaat tcaaaagacc aggaaaaaaa tgaagtaaga
    15781 gtttcctgtt tgtggaaact gctttgctat gaatacctta tactcctcta caaaacgggt
    15841 ttcttcccga taggaaaaac ccgtgtttta ctctcttagt aaatgtaatt ttgtcgccgt
    15901 atctttctct ctaatacacc agaaaatagt gtgctaactc taatgctttg cattgctcta
    15961 ttatttgagg gaattcaagt tatctgcgga tgcatagata tggtatccag tatgtacatt
    16021 gccattatgg ttgaaaggca ataccagggg caattggtca tttaaaaatc aagtggtaac
    16081 aactgaaatt tgaaaatttg tttatagtgg agagaacaat ttacaactta taaaaaattc
    16141 ttggtaatta acaacaagtt atcttat
//
LOCUS       DASLOE010000057        16053 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.057, whole genome shotgun sequence.
ACCESSION   DASLOE010000057 DASLOE010000000
VERSION     DASLOE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 16053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16053
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.057"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            170..364
                     /locus_tag="U6309_001457"
     CDS             170..364
                     /locus_tag="U6309_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504905.1"
                     /translation="MKKSTMAVATLLAALSTAPMVSVAYADDTSNTTTSSCTGCKGCK
                     GCNGCKGCGGCGGCGGCSGD"
     gene            435..1148
                     /locus_tag="U6309_001458"
     CDS             435..1148
                     /locus_tag="U6309_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504906.1"
                     /translation="MNSQSDFNLPILSSWYKKKFLGYASQVLEAQQKMTSSKGKNILH
                     YTLRKKRKHERMSHYPKGDRIDRSTGSQYFYHCHRENFESNEHGHFHCFLRYKHIPKR
                     IKPAPLEDWDKYIDNPMTHLVAIGMNQFGQPIRLFTVNRWVTSEIWYGAEHIPYFLKS
                     YKMTLIDDPYWQVLDQWVEGMLHLFAPQIAWLHQERDKRIQLHQLNSPNDNPYTNHEL
                     EELSEINIDLKKQIEWVIS"
     gene            1301..3643
                     /gene="purL"
                     /locus_tag="U6309_001459"
     CDS             1301..3643
                     /gene="purL"
                     /locus_tag="U6309_001459"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEN5504907.1"
                     /translation="MQNLTETLDFSSLNRGEIEKLLRDYNLNLSVDEALTIQNEFLKR
                     PPTISECVLWSIQGSEHCSYKSSRIHLKQFNTSGPHVILGAKEDAGIVSVAQDKNGYR
                     YGVIVSHESHNHPSQIVPYEGAATGVGGNVRDVCCMGGEVIAVADSLRFGDIKRARTH
                     WIQEGVVSGIAGYGNPLGIPNIAGDVYYDPAYNENCLVTVVTLGIVREDHIIHSYAPP
                     EAENYVFILVGKPTDNSGFGGASFASTVLEEDSQEKNKGAVQEPNAFLQRHLLKANYS
                     LFQLLREKNLIDRVGFKDLGAGGVACASIELAEAGGSYGAEIDLDKVPTGMPGLMPSV
                     ILCSETQERFMWVVPPDLVDTILKHYNETFALPQVSEGACAAVIGKIRTDGLYVVNYK
                     GRELVRAKVPDVTKGIVYNRPHASSQKQNTEPVFLPPDDYNQILLQLLAHENIASKEP
                     IFEMYDKQVQGRTLIQAGWADAGVLQPFNESKYPEEIRKTGIALSLDQNPRYNKIDAY
                     WGAVNAVVESVRNITAVGATPVAITDCLCFGNPEKPEQMREFVDSVRGISDACAAVHL
                     KDHPQSTLPVIAGNVSLYNESVKGAIPPSPMISCLGTLPDIDFAITFDFKKSDSLLIL
                     IGERKDECGGSVYYQLHNELGSNVPKPDLSLFNREIHAVSSAIQHGLVNAAHDVSEGG
                     VAVALAEMSFKNSLGVAVQINGELSADKLLFGETGGFILEIDKQHKAAFDKLVTQYQV
                     PYMVIGHTTEQPVLQMNSVINLPVEEARQAWENGLRERLL"
     gene            3643..4686
                     /locus_tag="U6309_001460"
     CDS             3643..4686
                     /locus_tag="U6309_001460"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEN5504908.1"
                     /translation="MSTINYKSAGVDIEAGNEAVARIKRNVEKTFSPQVLTGIGSFGA
                     MYDLKPFMEKFKHPVMVQSIDGVGTKTIVARMMQKFDTIGIDLVSATTNDIIVLGARP
                     LTLLDYIATDRLKPEYIEEIIIGMTQACLENNISLVGGETAEMPDTYLKGEHDLVGII
                     TGVVEKDEAILGKDITPGDSVLAFPSSGLHTNGYSLARKLLFELGGYTVESQLDELDA
                     TVGEALLVPHINYTNPVLHLLNNKIPIKGMAHITGGGVLENIPRVLPNHCSVEINKNS
                     CPVLPIFNVLKALGKLDDQEMYRTFNMGIGLVMIASPGVVSEIYTALKEFPNFPVYEV
                     GKVVSGKPEVRLL"
     gene            4686..5945
                     /gene="purQ"
                     /locus_tag="U6309_001461"
     CDS             4686..5945
                     /gene="purQ"
                     /locus_tag="U6309_001461"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HEN5504909.1"
                     /translation="MKIAVVQFPGSNCERETMLAVQRAGMTPVEFLWNDSSQKLREMD
                     GYIIIGGFSYEDRSRAGIIAALDPVMKEIKLQSELGKPVLGICNGAQILVETGLVPGL
                     KNYQIGMALTENKRIQDGKILGTGFYNSWIHMRTGDRVRQNAFTRYLSKDSVLSIPAA
                     HAEGRFVMPVKLLQEIEEEGLNAFQYCDAQGNRDDNFPVNPNGSLRNIAAIINKAGNV
                     MAIMPHPERTFAGDPIFHSMKEYIKEKKTFKQQTLSYQPEPVKIIPYDKKDNAHELIV
                     KLIITDNAALTVQNTLRQLGIPVTVNRMMYWHVDTNFPETIEKIKQTGVLYNDRKEYL
                     VTSEHIFSSAAKAFLVKSKDDMIGQQKLQMLRDHFSIDGVDAIHHGVLWMFSSENVKI
                     QELAESILATNIIFNPNAHICYEYQCP"
     gene            5926..6906
                     /locus_tag="U6309_001462"
     CDS             5926..6906
                     /locus_tag="U6309_001462"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEN5504910.1"
                     /translation="MNTNVPSQEDLLAALPFCLTETSLPFGKKYKGKVRDTYDLGDQL
                     ILVTTDRQSAFDRCLAAVPYKGQVLNLTSAWWFKNTQSIVPNHLIAVPDPNVAIAKKC
                     KIFPIEFVVRGYISGSTSTSLWTQYQKGVREYCGITFPDGLRKNQKLESPVITPTTKE
                     TLHDRPISPHEIVAEGWMTQEDWDETSSYALKLFQHGMEVAQQHGLILVDTKYEFGRD
                     AEGRIVLVDEIHTPDSSRYWLFNGYQERFDAGKEPENIDKEFLRLWFVDHCDPYKDEV
                     LPQAPQELIVTLASRYIQLYEMITGESFVYDSNPGPVNDRILHNIQRWLG"
     gene            6929..8428
                     /gene="purF"
                     /locus_tag="U6309_001463"
     CDS             6929..8428
                     /gene="purF"
                     /locus_tag="U6309_001463"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEN5504911.1"
                     /translation="MCGIVGIYSHEPVAAELYDSLIHLQHRGQDASGILTGADRFYFK
                     HGLGLVRESIFSEDILKLKGNIGIAHVRYPTAGGYSEADVQPLWIGSPRGIALAHNGN
                     LVNYGELVDDLCGKQHRHLNSTLDSEALLLLLADYLAKGSYSDHDEEQFFQLLCMAVN
                     EIHQRVQGSYSVVSLVIGKGLVAFRDPHGIRPLVWGERVHENGSKDYIFASETTPFYA
                     LGFEPKGDLQPGEVAYVNQQGQLYRKVLSSKKFTPCIFEYVYFARPDATLDDVSVYRA
                     RLRMGQNLAKSWKKRFPDTLPDVVIPAPSTANTAALAFANELGIRYSEGLYKNPFIGR
                     TFIMPNQESRSRQVRYKLTPQKTEINKKKVLIVDDSIVRGTTSREIVRMIREYGASEI
                     YFVSTCPPIKNPCFYGIDIPSRKKLIAAHKTEEEIRQFLGVDKLLYQTQENLIEAVSR
                     RGQYDMSKPCMACMDGKYICGQITEEKIKLLEKKRLTENDQETEIGEMS"
     gene            8425..9744
                     /gene="purD"
                     /locus_tag="U6309_001464"
     CDS             8425..9744
                     /gene="purD"
                     /locus_tag="U6309_001464"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEN5504912.1"
                     /translation="MINILIVGSGAREHAIATALSKSENKPKIYCFGTTRNPGIQRLT
                     SDYWVGDITDPEQVNQQAKEWEINLAIIGPEAPLEHAVADALWDCGIPVIGPRKNLAK
                     IETSKAFARELMEKYGIPGLPKYKVFTSLEGIGEFLSELGENNYVVKANGLMAGKGVK
                     VGGEHLLSFEEALNYCKEIFTHNQSLVIEEKLIGQEFSFMCFSDGISLVPMPIVQDHK
                     RAYEHDEGPNTGGMGSYSAADHSLPFLSPEDVRAAFEINQSVIKALMTEMSDKYIGIL
                     YGGFMATAHGVYVIEFNARFGDPEALNVLSLLESDFVKLCVAMANGNLQKESVRFLNQ
                     ATVCKYAVPDGYPDNPLRHVEIDISAVEPKDTLYLAAVNFKNGKLYATGSRTAAYVGI
                     ANTISEAEEIAEQQIKNIKGPLFHRKDIGTSPLINTRVEVMRRLRAL"
     gene            9741..10319
                     /locus_tag="U6309_001465"
     CDS             9741..10319
                     /locus_tag="U6309_001465"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEN5504913.1"
                     /translation="MIRLGILGSTRGTNMLALVDAINEGTLKAKIELVISNKPDAIIL
                     ERAKSLGLNAQFVNPEGLNRIDFDKKVSDILINHQIDLIVLIGYMRILSADFVNKWNN
                     QVINVHPSLLPAFAGKMDMDVHQAVLDSGLKETGCTIHFVTEEVDAGPVILQKKCPVL
                     EGDTAQTLKARVQQLEGIALVDAINLIASKGL"
     gene            complement(10316..12433)
                     /locus_tag="U6309_001466"
     CDS             complement(10316..12433)
                     /locus_tag="U6309_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEN5504914.1"
                     /translation="MGYHFLTEGNGIIACYDMYPTQFHSIKDMINYLPVLKQMGFNAL
                     WINPMQMPGDISGFFKTDKNNGVKTGNEVTRSLYAMSHPLLFNPQFSLGSPEDPMGTT
                     QRLNSEALQLFTQNARNLGIVPMFDLVLNHVAIDSPLCQDKPHWFKGVHEDFKDVRGF
                     NYDDESIRKEIITEFWQPYIKRYMIEYGFDGVRVDAVGYVYPAVRSEIYAYIHSLAAE
                     YGKPKPVILDEALFSKRPLADEVNYLKLPGIGPTHITTEAYNVEFDLASADLPYEITL
                     EEQLKASVVFQQKDGTWRENTKGGCINFCGNHDYRSLAMTILFQMAKKRLESDAFYND
                     LMSSYQDLYFKFNAEQEKPHELKEPLKTTLLYSYVDQIKKELEDNKDETTQEFKTLVL
                     EKLALSALTASGGWFLLSGDETCDITAKTVFQRKGAVDKSYYPQREHRIFSEKPVIAH
                     KILEKMAEENFFIENSENKWMQELYRSLSSFPEMQKRLLVSHIDNIKHQINAGIDHVQ
                     EKFSRLLASENLNIGFTSQDFLENPRTHENGWLGLQNNFLFIKQLNTLLKRLPASLPG
                     FSSELVRLPEKPHLIIIVRKNGNELNAPIDVVVVNLEQEKRQTLTKEDFQLIAKNYCN
                     QYFSKNRPSVCDSLREKLYSHVLKSAVNNRVHTDTSIKLNGIHLFEFKETSLNTFFTI
                     EKEKNYSHGNEDNTALTGNTISH"
     gene            complement(12537..13427)
                     /locus_tag="U6309_001467"
     CDS             complement(12537..13427)
                     /locus_tag="U6309_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1670 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504915.1"
                     /translation="MGSSYNDWLKSSYSELKKINIIENLIKENNFARAKMLLNNLDLT
                     TLIKYTELSKTITDFCEEAEQADIWRTHLQNFNEEHFSFEEYPPLTVSQLVKGIYFYG
                     QAAECREEEGKPFGDNELEFLKKSAYQHCFYAYNSLSTWAYEKYKMGLNDYSLLTLHY
                     AQKACQYHWTPGYLLFYKTCLNLAILSNAPSLSYQEALEALLIARKLSEHQYSISAIN
                     NAYFGKGLIHGNKTQIESWDKAISETIAKGKIPSTLLNKIYDKASEKAKGILDEFTVE
                     VKDMPEEEKLENEVAPTLSI"
     gene            13730..15955
                     /locus_tag="U6309_001468"
     CDS             13730..15955
                     /locus_tag="U6309_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEN5504916.1"
                     /translation="MAESKLEIVNKTEREESVILDGNGILRCYNMFPTQFGSIEEMID
                     YLPQLQAMGFNAVWINPVQTAGDVQGLFKRDKTIGVRAYNEVTRSLYAMTDCDLISPY
                     FNPIPPNVDPKLKKQLDMEAMQKFTSEARNNGLVPMFDLVLNHVAADSRHRKEHPEWF
                     QKDVHPDFKDAIAFDYSDPQIRDEIIEQFWKPYIYKYMIDYGFEGVRIDAVGYLNPEL
                     RKQIYAYIYDLAEQFNKPKPVILDELLFSGSQKLTDVVDGLLLPEKGPTHITRGTYYA
                     QRDGYGGLPGWCKEEEGVKAQVVFLDKQKKLHPHTKGGCIAFSGNHDHNSLAMTILEE
                     MAEHRLANHHTLHGAQEKFKHERSYDYDEALESVLRYSFVKDIQNEILNGNESTIKEV
                     ETRMREKIAFCALTSSGGWYALSGDETGDLLAKPVFRRVHALDQTYYAQRTHIIFDKK
                     YPDNKVAMEVLTEMAIENIQKEKAGSIYNSLQHDKQSQERLLVPYLETLQNQINCGDT
                     VVCKAFQEKLAQKNITIGFTEENYNPAPRTPQNGWNGQHDMKKFMFEINSILSSLPSS
                     NMGFWSEVIPIQSKPGLLAVVRKNGLGLSSETDIAIVNMDPSQKVELTRDDIHQLACN
                     FQKRVIPEYTYYNDGKDKQYNWHTNNPDFNMAYHTVMSCIEAKRIHVDDSIKTNFPVH
                     SANPYGMFSKKQSSKMNIAKIAKMSGEDTTLIEKGTNDTEGIIHDDEQIQPTITVEIK
                     K"
BASE COUNT         5030 a         2743 c         3348 g         4932 t
ORIGIN      
        1 cttgtataag caagaattta tcaaacccgt gtttataggc atataaaaaa tcaattttat
       61 taaaaaataa tcaattaatt tttgtgaaaa aagaagaaaa attttgattt gtttgctaat
      121 atcttaatgg gtaaataaaa aatccattaa ctatcaagga agtaatacta tgaaaaaatc
      181 aacaatggca gtagccactc tgttagctgc tttatcaaca gcacctatgg tttccgtagc
      241 ttatgctgat gatacttcaa atacaacaac ttctagctgt actggttgta aaggatgcaa
      301 gggctgcaac ggttgcaaag gctgtggcgg ttgtggtggc tgcggtggtt gcagtggtga
      361 ttaataagta atattttcta taaagaggta gtgttaacta cctcttttta ttacggttta
      421 ttttttaagt aattatgaat agtcaatcag attttaactt acccatattg tccagctggt
      481 ataaaaagaa atttcttggt tatgcttcac aagttcttga agcccaacaa aaaatgacat
      541 ccagtaaagg taaaaatatt ctgcactaca cgcttagaaa aaagcgaaag catgaacgca
      601 tgagtcacta tcctaaggga gacaggattg atcgtagtac tggctcacaa tatttttatc
      661 attgccatag agaaaatttc gaaagtaatg aacatggtca ttttcattgt tttttgcgat
      721 ataaacatat accgaagcgt attaaaccag ctcctttaga ggattgggat aaatacatag
      781 ataatcccat gactcacctt gttgcgatag gtatgaatca gtttgggcaa ccgatacgat
      841 tatttacagt caatcgttgg gtaacatcag aaatttggta tggtgctgag catattccat
      901 atttcctgaa atcttacaag atgacattaa tagacgatcc ttattggcaa gtattagatc
      961 agtgggtgga aggcatgctc catttgtttg ctccccaaat tgcctggctt catcaggagc
     1021 gagataaacg cattcagctt caccaactga atagcccaaa tgataacccg tacactaacc
     1081 atgagctgga agagctttct gagataaata ttgatttgaa aaagcagatt gagtgggtaa
     1141 taagttaaat tccaaaatta gaaccacaac ttaatttatc ttaattattg gtttttcatt
     1201 gcttataccc cgtgtaaaaa tatacaatat ctactgcttt ggggagagat tagtccttta
     1261 ctgatcaatc agagattaat tctatctggt atagagattc atgcaaaatc ttactgaaac
     1321 ccttgatttt tcttctttga atcgtggaga aattgaaaaa ttactgcgcg attataattt
     1381 aaatctttct gtagatgagg cgttgactat acagaatgaa ttcctcaaaa gacccccgac
     1441 tatttcagag tgtgtacttt ggtctattca aggatcagaa cattgttctt ataaaagcag
     1501 ccgtattcat ttaaagcaat tcaatacctc aggccctcac gtgatactgg gcgccaagga
     1561 ggatgcaggt atagtatctg tagcccaaga taaaaatggt tatagatatg gcgttattgt
     1621 cagtcatgaa tcacataatc acccatctca aattgttccc tatgaaggcg cagctaccgg
     1681 agttggtggt aatgtccgtg atgtctgttg tatgggtggg gaagttatag cagtagcaga
     1741 cagtttgcgt ttcggagata tcaaacgtgc acgtactcac tggatacagg aaggggtggt
     1801 ttcaggtatt gccgggtatg gcaatccttt gggaataccg aatatagctg gtgatgtgta
     1861 ctatgatccg gcctacaatg aaaattgttt ggttactgta gtcactctgg ggatagtaag
     1921 agaggatcat attatacatt cttatgctcc gcccgaagct gagaactatg tttttatttt
     1981 agtaggtaag ccaactgata atagtgggtt tggcggcgcg agctttgcct caacagtatt
     2041 ggaagaagat tcacaagaaa agaataaggg agctgttcaa gagcccaatg cttttttaca
     2101 gagacatctc cttaaagcta attattcatt atttcaatta ctaagagaaa agaatttaat
     2161 tgacagggta ggctttaaag atttaggagc aggtggcgtg gcctgcgcaa gtatcgagtt
     2221 ggctgaagcc ggcggttctt atggcgccga aattgatctg gacaaggtac ccacaggaat
     2281 gccaggatta atgccttcag tcattctatg ctctgaaact caggagcgtt ttatgtgggt
     2341 tgttccccct gatttagtag atactatcct gaaacactat aatgaaacct tcgcgttacc
     2401 acaggtttct gaaggggcat gcgctgcagt tatcggcaaa attcgtaccg atggattata
     2461 cgtggtgaat tacaaagggc gagaacttgt gagagctaaa gtccctgatg tcacgaaagg
     2521 cattgtttat aacaggccac atgcctcttc gcagaagcaa aacacagaac cagtttttct
     2581 cccaccagat gattataatc aaattttgtt gcagttattg gcccatgaga atatagcgag
     2641 caaggaacct atttttgaaa tgtatgacaa gcaagtgcag ggtcgcaccc tcatacaagc
     2701 aggatgggct gatgctggag tattacaacc atttaatgaa tcgaagtatc ccgaggaaat
     2761 tcgtaaaaca ggtattgcct tgtctttaga tcaaaatccc cgctataaca aaattgatgc
     2821 ctattgggga gctgttaacg cggttgtcga atcggtacgg aacatcactg cagttggagc
     2881 tactccagtc gcaataacag attgcttgtg ttttggtaat ccggaaaagc cagaacaaat
     2941 gcgcgaattt gttgattctg tgcgaggaat tagtgatgcc tgtgccgctg ttcatctgaa
     3001 agatcatccc caatcaactt tgcctgtcat tgccgggaat gtctcattgt ataatgaatc
     3061 agtgaaaggg gctattcctc ccagccccat gattagttgc ctgggaacgc ttccagatat
     3121 tgattttgct attacctttg attttaagaa aagtgatagc ctattgatat tgattggaga
     3181 gcgtaaagac gaatgtggtg gtagcgttta ttatcaactc cataatgaat tgggttctaa
     3241 tgttccaaaa ccggatttaa gcttgtttaa tagagaaatc catgctgttt catcggcaat
     3301 tcagcatggg ttggttaacg ctgctcatga tgtctcagag ggaggggttg ctgttgctct
     3361 tgctgagatg agttttaaga actccctggg agttgctgta cagattaatg gagagttatc
     3421 tgcggacaaa cttttatttg gagagacagg cggttttatt cttgaaatcg ataaacaaca
     3481 taaagccgct tttgacaaac tggttactca atatcaagtc ccttacatgg ttatcggaca
     3541 tactacagag cagccagttt tacagatgaa ttcggttatc aacttgcctg tcgaagaagc
     3601 gaggcaggct tgggaaaatg gcttaagaga gaggctatta taatgtctac tatcaattac
     3661 aaatcagcgg gtgtcgatat cgaggcggga aatgaagctg tcgcacgcat taaacgtaat
     3721 gttgaaaaaa cattttcgcc tcaagtcctg acaggaatcg gtagttttgg cgcaatgtat
     3781 gatttgaagc cttttatgga aaaattcaaa caccccgtca tggttcaaag cattgatggc
     3841 gtaggcacga agacaatagt tgctcggatg atgcaaaaat ttgatacaat cggaattgat
     3901 ttggtcagtg cgaccaccaa cgatattatt gtgttgggtg caaggccatt aacacttttg
     3961 gactatatag caactgatcg attgaaacct gaatatattg aagaaattat tattggcatg
     4021 acgcaagctt gcctcgaaaa taatatttct ctggtcggtg gtgagacggc tgaaatgcct
     4081 gatacctatc tgaagggaga gcatgatttg gtaggtatta tcactggtgt agtggaaaag
     4141 gatgaagcca tactaggaaa agatataact ccaggcgatt cagtcttggc atttccttcc
     4201 agtggcttac ataccaatgg atattcactt gccagaaaac ttttgtttga actaggtgga
     4261 tatactgtag aatcccaact tgacgagttg gatgctacag ttggtgaggc tttactggta
     4321 cctcatatca attatacaaa tcctgttcta cacctgttga ataacaaaat ccctatcaag
     4381 ggaatggctc atattactgg cggaggtgta ctggaaaaca ttccgcgagt attacctaat
     4441 cattgttccg ttgaaatcaa taagaattca tgtccggtgt tgccgatctt taatgtgcta
     4501 aaagcattgg ggaaactgga tgatcaggaa atgtatcgta cttttaatat gggaatagga
     4561 ttagtaatga tagcttcccc tggtgttgtt tctgaaattt atacagcgct aaaagaattc
     4621 cctaatttcc ctgtttatga agtaggaaaa gtcgtatcag gaaaaccaga agtgagatta
     4681 ttgtgatgaa gattgcagtt gttcagtttc caggctccaa ttgcgaacgt gagacaatgc
     4741 ttgcggtgca acgagcaggt atgactccag ttgagtttct gtggaatgat tcatcacaaa
     4801 aactgcgaga aatggatggt tatattatta ttggtggttt ttcatatgag gatagatcaa
     4861 gagcaggaat tattgcagcc cttgatccgg taatgaaaga aataaaattg cagagtgaat
     4921 tgggaaaacc tgttcttggt atttgcaatg gagcacagat ccttgttgaa acaggcctcg
     4981 ttcctggttt aaaaaactat caaattggta tggctctgac tgagaataag cgaattcagg
     5041 atggaaaaat tttaggaaca ggattttata acagctggat tcatatgcga accggtgacc
     5101 gagtgaggca aaatgcattc acccgctatt tatcaaagga ttctgtatta tcaattcctg
     5161 ctgcccatgc tgaagggcgt tttgtgatgc ctgttaaatt attgcaagaa attgaagaag
     5221 aaggtttaaa tgcgtttcaa tattgtgatg cgcagggaaa cagagatgat aatttccccg
     5281 ttaatcctaa tggttctcta cggaatattg ccgctattat taataaagcg ggtaatgtca
     5341 tggccataat gcctcaccct gaacgtacat ttgcgggaga tcctatattc cattcaatga
     5401 aagaatatat taaggagaaa aaaacattca agcagcaaac attatcttat caaccggagc
     5461 cagttaaaat aattccttat gacaaaaaag acaatgccca tgagttgatt gttaaattga
     5521 ttattaccga taatgctgct ttaacagtac aaaatacgct aagacagtta ggtataccag
     5581 tcacagttaa tcgtatgatg tattggcatg tcgatacaaa ttttcctgaa acaatcgaaa
     5641 aaataaagca gacaggggtt ttatataatg atcggaaaga gtatttggta acgtctgagc
     5701 atatattttc atcagcagcc aaggcttttt tggttaaatc aaaagacgat atgatcgggc
     5761 aacagaagct gcaaatgctt cgggatcatt tctcaattga tggcgttgat gccatacacc
     5821 atggtgtttt gtggatgttt agcagtgaaa atgttaaaat acaagaattg gcagaatcca
     5881 ttttagctac taatattatc tttaacccta atgctcatat ctgctatgaa taccaatgtc
     5941 cctagtcaag aggatcttct tgctgcgttg cctttttgct taaccgaaac ctctttacct
     6001 ttcggcaaaa agtataaagg gaaagtaagg gatacctatg atctcggcga tcaattgatt
     6061 ttggtaacta cagatagaca aagtgccttt gatcgatgcc ttgctgctgt accttataaa
     6121 ggacaagtat tgaatttgac ttcagcatgg tggtttaaaa acacgcaatc gattgtgcct
     6181 aaccatttaa ttgccgtacc tgatcccaat gtggcgatag ccaaaaaatg caaaatattc
     6241 cctattgaat ttgttgttcg tgggtatatt tcggggagta caagtacgtc tttatggacg
     6301 caataccaaa aaggagttcg tgagtactgt ggtataacgt ttcctgatgg attaagaaaa
     6361 aatcaaaaac ttgagagccc agtgataacc cccactacta aagagacgct ccatgacagg
     6421 ccgattagcc cacacgaaat cgtcgcagaa ggctggatga cccaagaaga ttgggatgaa
     6481 accagcagtt atgctttaaa gttatttcaa catggaatgg aagtagcaca gcaacatggt
     6541 ttgattttgg tggataccaa atatgaattt ggccgtgatg cggaaggaag aattgtatta
     6601 gtggatgaaa ttcatactcc agattccagt cgttattggt tatttaacgg ttatcaagag
     6661 cgatttgatg ccggtaagga gcctgagaat atagataaag aatttttgcg cttgtggttt
     6721 gttgatcact gcgatcctta taaggatgaa gtattacctc aagcaccaca ggagttaatt
     6781 gttactttag catcaagata tatccaatta tatgaaatga ttacaggtga atcgtttgtg
     6841 tatgacagta atcctgggcc agtaaatgat aggattttgc ataatataca acgttggtta
     6901 ggttaatttt tagccggggg aaaggcttat gtgtggcatt gtaggaattt acagtcatga
     6961 acccgttgct gctgagcttt atgatagctt gattcattta cagcaccggg ggcaagatgc
     7021 ctcaggtatt ttaaccggag ctgatcgttt ttacttcaaa catggacttg gactggtcag
     7081 agagtctatt ttctctgaag atattcttaa actgaagggg aatatcggca ttgctcatgt
     7141 gcgttatccc acggccggag gttatagtga agcggatgtt cagcctcttt ggatagggag
     7201 ccctcgcggt atagctttgg ctcataatgg aaatttagtg aattatggag agttggttga
     7261 tgatttatgc ggaaaacagc ataggcattt aaactctact cttgattctg aggcactttt
     7321 attacttctg gcagactatt tagccaaggg atcttattcc gatcatgatg aagagcaatt
     7381 ctttcaatta ctatgtatgg cagttaatga aatacatcaa cgagttcagg gttcctattc
     7441 tgttgtgagt ttagtaattg gcaaaggttt ggttgcattt agggatccgc atggtatccg
     7501 tcctttagtt tggggtgagc gagtccatga aaatggtagt aaagattata tttttgcttc
     7561 agaaaccacc cctttttatg ctttaggttt tgagcccaag ggtgatttgc agcccggtga
     7621 agttgcttac gtgaatcagc aaggacaatt gtacagaaaa gtgctttcat caaaaaaatt
     7681 taccccttgt atatttgaat acgtttactt cgccagacca gatgccacat tagacgatgt
     7741 gagcgtttat cgagccagat tacgtatggg gcaaaattta gctaaaagct ggaaaaagag
     7801 atttccagat acactacctg atgtggtgat tccagcccct tcaacagcaa atactgccgc
     7861 attagctttt gcaaatgagt tgggaatacg ttattccgaa ggtttatata aaaatccttt
     7921 tattggacgt acttttatta tgcctaatca ggagtcgcga tcaagacagg ttcgttataa
     7981 attaactcct cagaaaacgg aaattaataa aaaaaaggta ttaatcgttg atgacagtat
     8041 cgtaagggga actacctcac gcgaaatagt cagaatgata cgagaatatg gtgcatcaga
     8101 aatatatttt gtatcaacct gtccaccaat taaaaaccct tgtttctacg ggattgatat
     8161 cccatcgcgt aaaaaattga tagcagccca taaaacggaa gaggaaatcc gtcaatttct
     8221 aggggtggat aaattattat accaaactca ggaaaacctt atagaggctg tgtctcgcag
     8281 ggggcaatat gacatgagca agccttgtat ggcttgtatg gatggtaaat acatttgtgg
     8341 acagattact gaagaaaaaa ttaaattgtt ggagaaaaaa agattaaccg agaatgacca
     8401 agaaacagag ataggagaga tgtcttgatc aatatcctta ttgttggttc aggtgcgcgt
     8461 gagcatgcaa tagctacagc attgagtaaa tcagagaata aaccaaagat ttattgcttt
     8521 ggaacaactc gtaatcctgg tatccaaaga ctcacctcag attattgggt aggtgatatt
     8581 acagaccctg agcaagttaa ccagcaagca aaagaatggg aaattaattt ggccattatt
     8641 ggtccagaag cgcctttgga acatgctgtt gctgatgctt tgtgggattg tggaattcct
     8701 gttataggcc ccaggaaaaa tttggctaaa attgaaacca gtaaagcgtt cgccagagag
     8761 ttgatggaaa aatacggtat tccaggtttg cctaagtata aagtatttac cagtttagag
     8821 ggaatagggg agtttttatc agagttaggt gaaaataatt acgttgttaa ggccaatggt
     8881 ttaatggcgg gcaagggtgt aaaagtaggt ggagaacatc tgttgtcatt tgaagaggct
     8941 ttaaactatt gtaaagaaat ttttactcat aatcagtctt tagtcattga agaaaaatta
     9001 ataggtcaag aattttcatt tatgtgtttt tctgatggca tctcattagt tccaatgcct
     9061 attgtgcaag atcataaacg agcttatgag catgatgagg gtcctaatac aggtggtatg
     9121 gggagttatt cagcggcaga tcacagcttg ccatttttgt ctccagagga tgttcgtgct
     9181 gcttttgaaa ttaatcaatc agtaatcaaa gcgcttatga cggaaatgtc tgataaatac
     9241 ataggaatac tttatggcgg atttatggcc accgcacatg gggtgtatgt gattgagttt
     9301 aatgctcgct ttggagatcc tgaggctctt aacgtgctta gcttgttgga atctgatttt
     9361 gttaaattat gtgttgcgat ggctaacggc aatttacaaa aagaatccgt tcgctttttg
     9421 aatcaagcca cagtttgtaa atacgctgtc cctgatggtt atcctgataa tccattgcgc
     9481 catgttgaaa ttgatatttc tgctgtcgaa cctaaagata ctctatattt ggctgcagtg
     9541 aattttaaga atggcaaact ctatgccaca ggttcgagga cagccgctta tgtcggtata
     9601 gcgaatacta tcagtgaggc agaagaaatt gctgaacaac agataaaaaa tataaaaggc
     9661 cctttatttc acagaaaaga tattggcaca tcgcctttaa ttaatacacg cgttgaagta
     9721 atgcgtaggt tgcgtgcctt atgattcgtt taggaatatt aggatcgact cgtggaacca
     9781 atatgttggc tcttgttgat gcaatcaatg agggaactct taaggcaaag atagagcttg
     9841 ttattagtaa taaacccgat gccattattc tggaaagagc caaatcgttg ggtttgaatg
     9901 cacagtttgt caatcctgag ggattaaaca gaattgattt tgataaaaaa gtaagtgaca
     9961 tactgatcaa ccatcagatt gatttgattg ttttgattgg gtacatgaga attttgtcgg
    10021 cggatttcgt aaataaatgg aataatcagg taattaatgt acatccctct ttattgccag
    10081 catttgcagg gaaaatggat atggatgtac atcaagctgt tcttgattcc ggcctaaagg
    10141 aaacaggttg tactatccat tttgtcaccg aagaggtaga tgcgggtcct gttattttgc
    10201 aaaaaaaatg ccctgtttta gaaggagata ctgctcaaac cttaaaagca agggtgcagc
    10261 agttggaagg aatagctttg gtagatgcaa ttaacctgat tgcatcgaaa ggtctctaat
    10321 gcgagattgt atttccagtc aaagcagtat tgtcttcatt tccatgagaa tagttttttt
    10381 ctttttcaat tgtaaaaaaa gtgtttaacg aagtttcttt aaactcaaat aaatggattc
    10441 cattcagttt aatactggtg tctgtatgga ctcgattatt tacagcagat tttaaaacat
    10501 gggaatataa tttttcgcgt aaactatcgc aaacactcgg tctattttta gaaaaatatt
    10561 gattacaata gtttttggca attaattgaa aatcttcttt agttaatgtt tgtcgttttt
    10621 cctgttcaag gttaacaaca acaacatcaa ttggagcatt taattcatta ccattttttc
    10681 ttacgataat aatgagatgc ggtttttcag ggagtcgaac caactcactg gagaatccgg
    10741 gtagtgatgc aggtagtctt tttaataatg tatttaattg tttgataaat agaaaattat
    10801 tctgtaatcc aagccatcca ttttcgtggg ttcttggatt ctctaaaaaa tcttgagatg
    10861 tgaaacctat atttaaattt tcactggcaa gtagcctgga aaatttttct tgtacatgat
    10921 ctatgccggc atttatttgg tgtttaatat tatcaatatg agatactaaa agacgttttt
    10981 gcatttcagg aaaacttgat aaacttctgt aaagttcctg catccactta ttttccgagt
    11041 tttctatgaa gaaattctct tctgccattt tttctaaaat tttgtgagca ataactggtt
    11101 tttcactaaa aatacgatgt tcacgttgtg ggtaataaga tttatcaaca gcaccttttc
    11161 tctgaaatac tgtttttgca gtgatatcgc atgtttcatc accagataat aaaaaccatc
    11221 cgccactggc tgtaagagcg gataaagcca gtttttctaa taccagagtt ttaaattctt
    11281 gtgttgtttc atctttatta tcttccaact cctttttgat ttgatcaaca taagagtaaa
    11341 gtaaggttgt tttcaaaggc tccttgagtt catgtggttt ttcttgctcg gcgttaaact
    11401 taaagtacaa gtcttgatag gaagacatta agtcattata aaaagcgtca gattccagtc
    11461 tttttttggc catttgaaat aaaatagtca tcgccaatga gcgatagtca tgattcccac
    11521 aaaaattaat gcagcccccc ttcgtgtttt cacgccatgt tccatctttt tgttggaaga
    11581 ctacactcgc ctttaactgc tcttccaatg ttatttcata aggcagatca gcgctggcaa
    11641 gatcaaattc aacattataa gcttctgtag tgatgtgagt tgggcctatg ccgggtaatt
    11701 tcaaataatt tacttcatcc gcaagtggtc gtttactaaa taaagcttca tcaaggataa
    11761 ccggctttgg tttaccatat tcagcagcaa gagagtgaat ataggcatat atttcacttc
    11821 ttactgcagg atatacataa cctacagcat cgactcgaac accatcaaaa ccgtactcga
    11881 tcatgtaacg tttgatataa ggttgccaaa attcggttat tatttctttt cttatgcttt
    11941 catcatcgta attgaagccg cgaacatctt tgaagtcttc atgaactcct ttgaaccaat
    12001 gaggcttatc ctgacacaag ggagaatcaa tggcgacatg atttaaaacc agatcaaaca
    12061 ttggaactat tccgaggttc cttgcatttt gagtgaataa ctgtaaagct tctgagttta
    12121 atcgttgggt tgtccccatg gggtcttcag gggagcccaa actgaattga ggattaaata
    12181 ataacgggtg actcatggca tataagcttc tagtgacctc atttcctgtt tttacaccat
    12241 tatttttatc cgttttaaaa aagccgctaa tgtcaccggg catttgcatg ggattaatcc
    12301 ataaggcatt aaaccccatc tgctttaaga caggcaaata attaatcatg tccttaatag
    12361 aatgaaactg agttgggtac atgtcataac atgcaataat cccatttcct tctgttaaaa
    12421 aatgatatcc catagaatcg atcaccgtag aaaattatta aactacaaaa ctgagaaaaa
    12481 tcgaattgtt ggttacaact ccacttttat cagtcgaatc gcttgctatt atagcgttaa
    12541 atagacaaag taggagctac ttcattttcc aatttttctt cttctggcat atcttttact
    12601 tcgactgtga actcatccaa tatccctttg gctttttcag atgctttatc gtaaatttta
    12661 ttaagcagag tagaggggat tttgcctttg gcaatagttt ctgatattgc tttatcccaa
    12721 gactctatct gcgttttatt gccatgaatc aggcccttgc caaagtaggc attattgata
    12781 gcacttatag aatattggtg ctcagataac tttcgggcaa tcaagagcgc ttctaatgcc
    12841 tcctggtaag ataaagaagg ggcgttcgat aaaatggcta aatttaaaca agtcttataa
    12901 aataataaat atcctggagt ccaatgatat tgacaggcct tttgagcata gtgcaaggtt
    12961 aacaaagaat aatcattcaa acccattttg tatttttcat aagcccaggt tgataaagag
    13021 ttataagcat aaaaacagtg ttgataagcg ctctttttaa gaaactccaa ttcattatca
    13081 ccaaatggtt taccttcttc ttcccggcat tcagctgctt gtccataaaa atagatccct
    13141 tttactaatt gggacactgt gagtggcggg tattcttcaa atgaaaaatg ttcttcgtta
    13201 aaattttgca gatgagtccg ccaaatatca gcttgctctg cttcttcaca aaaatctgta
    13261 attgttttac ttaattcggt gtatttaatg agggtagtga gatcaagatt atttaagagc
    13321 attttcgccc tggcaaaatt gttttctttt atcagatttt cgatgatgtt tattttcttt
    13381 aattcgctat atgacgattt cagccaatca ttataggagc tacccataaa tcctccacaa
    13441 aatttttaag ttcaagacat gttagagatt aaagtctaag tcttgacacc aatatttgtt
    13501 aataattggt gatataaata cggtaatatt ttaacacaat ttgatttatt taaaaacttc
    13561 taatatcaat ttagccgctc aataaccttc aaaaatgaga aaaattacaa aataacttcc
    13621 cctattgatt atacaacggc tatgtttgct tgatttttaa gctcaagagg cataatcctg
    13681 agatgtgtac tatattttat ctaagagata atgatatggg catattatta tggcagaatc
    13741 aaaattagag atagtcaata aaacagaacg tgaagaaagt gtaatcttgg atggtaatgg
    13801 gattttacgt tgttataaca tgtttcctac tcaatttgga agcatagagg aaatgattga
    13861 ctatcttcct caattacagg ctatgggatt taatgctgta tggataaatc ctgtacaaac
    13921 agcaggggat gttcagggtt tatttaagcg tgataagaca attggcgtca gagcttataa
    13981 tgaggtgaca agaagtctat atgctatgac agattgtgat ctgataagtc cttattttaa
    14041 cccgatccct cccaatgttg atcctaagtt gaaaaagcag ctggatatgg aagcaatgca
    14101 gaagttcaca tcagaagccc gcaataatgg tttggtaccg atgtttgatt tggttttaaa
    14161 tcacgttgcc gccgattcac ggcacagaaa agaacatcct gaatggtttc aaaaggatgt
    14221 ccaccctgat tttaaggatg ctatcgcttt tgactacagt gaccctcaaa tccgagatga
    14281 aattattgaa caattttgga agccttatat ttataagtac atgattgatt atggctttga
    14341 gggtgttcgc attgatgctg tgggctattt aaatccagaa ttacgcaaac aaatttatgc
    14401 ttatatttat gatcttgcgg agcagtttaa taaacctaaa cccgttatat tggatgaact
    14461 tcttttcagt gggagtcaga agcttaccga tgtggttgat ggtttgttgc ttccagaaaa
    14521 agggccaacc catattactc gcggtaccta ttatgcgcaa agagatggtt acggtggttt
    14581 acccggatgg tgtaaggagg aggagggagt taaggctcaa gtagtattcc tggataaaca
    14641 aaagaaattg catccccata ccaaaggcgg atgtattgct ttctccggta atcatgatca
    14701 taactcctta gccatgacaa tattggaaga aatggcagaa caccgtttgg caaatcatca
    14761 cactttgcac ggtgcgcaag aaaaatttaa acatgaaagg agctatgatt atgacgaagc
    14821 tttagagtca gtcctgcgct attcttttgt gaaagatatt caaaatgaga ttctaaacgg
    14881 taatgaatca acaatcaaag aagtagaaac aagaatgcgt gaaaaaatag ctttctgtgc
    14941 tttaacatca agcggcgggt ggtatgctct atcaggtgat gaaacaggcg atttattggc
    15001 aaaacctgta ttcagaaggg tccatgctct ggatcaaacc tattatgcac aacgcactca
    15061 tataatcttt gataaaaaat atcccgacaa caaagtagct atggaggtat taaccgaaat
    15121 ggctatagag aatatccaaa aagaaaaagc tggaagcatc tataacagtt tacagcatga
    15181 taaacaatca caagagagat tattagttcc ttatctggag actttgcaaa atcaaattaa
    15241 ttgtggtgat acagtagttt gcaaagcatt tcaagaaaaa cttgcccaga aaaatataac
    15301 gattggtttc acagaagaaa attataatcc cgctcccaga acacctcaaa atggttggaa
    15361 cggtcaacat gatatgaaaa aatttatgtt tgaaatcaat agtatattaa gttccttgcc
    15421 aagttccaat atgggatttt ggagtgaagt tatcccaatc cagtcaaaac cagggttgct
    15481 tgcagtagta cgtaagaatg ggctaggctt aagttctgaa actgatattg caatcgttaa
    15541 tatggatcca agtcaaaaag ttgagttaac cagagacgat attcatcaat tagcctgtaa
    15601 tttccagaaa cgagtaattc ctgaatatac ttattacaat gatggtaaag acaaacagta
    15661 taattggcat acgaataatc ctgattttaa tatggcttat catacagtga tgtcctgtat
    15721 cgaagccaaa aggatacatg ttgatgattc gatcaaaact aatttccctg ttcattcagc
    15781 aaatccctat ggcatgttta gcaaaaagca atcatcgaaa atgaatatag ccaagatagc
    15841 aaaaatgtct ggggaagata ccactttaat tgaaaaaggt actaatgata ctgagggtat
    15901 catacacgat gatgagcaaa ttcaacccac aataactgta gaaatcaaaa aataaagaag
    15961 tactataata aaagttgttt caacttggcg acagggtatt attttgataa atcggatact
    16021 ctgcaatata actaattaca aataccgtat aac
//
LOCUS       DASLOE010000058        15896 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.058, whole genome shotgun sequence.
ACCESSION   DASLOE010000058 DASLOE010000000
VERSION     DASLOE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15896
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.058"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            69..572
                     /locus_tag="U6309_001469"
     CDS             69..572
                     /locus_tag="U6309_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504917.1"
                     /translation="MSIFKTIKNIIYNPNVYIAVVIGALFGGVSGGAVGLFSGGFIGR
                     SFKICMDCPNQLLGFNIGIFDLNMVAGAIIGVVIGAALGGAITGLITSFHVYRKPHLP
                     KTVSRDNIHEVLISALWISIEISIGIILGAVIGSLNSPGIGSVTGALIGIILMLLTTI
                     WENRAKK"
     gene            complement(621..1115)
                     /locus_tag="U6309_001470"
     CDS             complement(621..1115)
                     /locus_tag="U6309_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEN5504918.1"
                     /translation="MGSLRKHQPGKTTDLLMSPFLRLQQEVNHAMSDFYNLFDPQRSA
                     NLEQFEHISLTPSLDIVEDKDNFKIEVEMPGMGEEDIKVSFCENRLTIEGEKTTSKKD
                     ENKNYISREISYGRYERTISLPLSADVDKATASFKKGMLWITIPKKTEAKENVKTIKI
                     EKAK"
     gene            complement(1377..2363)
                     /locus_tag="U6309_001471"
     CDS             complement(1377..2363)
                     /locus_tag="U6309_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN5504919.1"
                     /translation="MHAMIMEKPGCALQYQEVNKPNPNENELLIKVSACGICRTDLHV
                     VDGELKNPKTPLIPGHQIVGTVEQVGSAVKKFAIGQRVGVPWLGGSCGICNFCTSGRE
                     NLCDQACFTGYQIDGGFAEFCVANYHFCFPIPDGYPDHQAAPLFCAGLIGYRALLKAG
                     NATHLGLYGFGAAAHILIQVACQKGIKIYAFTSPGDQETQKFAYRLGAVWAGDSTQSP
                     PHLLDAAIIFAPVGALVPQALHNTVKGGTVVCAGIHMSDIPSFPYEILWGERTVCSIA
                     NLTRKDGEEFLSLAPKIPVKTKVQTYPLTEVNRALDDLRHGRYRGAGVIVMK"
     gene            2625..3188
                     /gene="thpR"
                     /locus_tag="U6309_001472"
     CDS             2625..3188
                     /gene="thpR"
                     /locus_tag="U6309_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEN5504920.1"
                     /translation="MNAIRAFFAISLPPAIQENIRSNLEPLRQMAPVHSIRWTKPMHL
                     HITLQFLQRLETKHIQILIERVSNELNHIKQFDLEFGVLEWFPSPKNPRLISLSVGPQ
                     DRLRDLSRMIGQAIGGLNYPVEKRPFRGHLTVGRVVNFSPKLQQLLQIKFPIIPPVPI
                     NKFYLIESRPDKEGSNYFPLAQFDLTR"
     gene            complement(3313..7197)
                     /gene="lepB"
                     /locus_tag="U6309_001473"
     CDS             complement(3313..7197)
                     /gene="lepB"
                     /locus_tag="U6309_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS GTPase-activating effector LepB"
                     /protein_id="HEN5504921.1"
                     /translation="MLTYQGKEIVRFKEKTGGKNKSDVDGFYKDSDGRKFFIKKPGDP
                     RELFTELFAGLLLKEFMKRGLIDESYFPSLICADVIQFEDKSYGLIQPLVSFDELHKI
                     IGTSYGDGSDRNTIKETFFGPGYYAGITKQNKYFGLSMALMFSLLLGAHSVHSGNIVV
                     LKGEGEEKSKQFGRIDWGDAFRYFAHPNNNDNLLYAYENRGWFNYKSLTKDYFLNYKK
                     INGLFPAMAEKARQLQSKLNPELLIEIVTSALQNIPADLIEEKTKIQLAAYMCMDSFK
                     EATFGPKGNCKDFAIAMATLLENRLGKIAVLKDMSPLSNPEELYQSILELKYMTLPMT
                     SSTSFSETINQWTNILKTSDMEKFSFDSNPINLLELVKQFNLYVDELAITCEANNVWA
                     KERIDSTPNLFALYDNSGGEAIHGHAFVPYYKESIVLRRLFTVDPNTFNLSRFAAFEG
                     PCQLYCKEHKDSAWVKIQTLLTLGNGIINTLKIIKQAQAFGIDEAVTENLKALKEQFI
                     AFQLAEADVKESLKAPTFAEPLPDKESEFFYPIDEKALAKMNGYQLATICLEELNSPK
                     PSPLIERILSNKKFWKRINSAFESGVFKGRTDDPAGKIAKIREWHQLLQISGKKTAGQ
                     IDELQKIVISLQSKIQKQTIEFEELEATLIQIKEKYQLQEKMAEQSEHEKSSAQSIIR
                     SLNLELSQLKLQLQEQEKLQFQLKELKEKIHEQTTLSKRLGEKLQTQKKTNTHQEETI
                     QRITKEKSLADSSLESLRKELHELAKKESSLHKTLEEKQLQVQQLEEQLAEKEKENLV
                     LKKADKQSQHEKSLDKSAIESLTSELNQLKLELQKQEALQLQLKSLSEQVQEQTLLVE
                     SLKEELQKQKESNTHQEETIERITKEKSLANSALESLRKEMHELTRKNEENQLKLTKQ
                     VHSLSEQLEEKQLQIREFEKQLQEKEKRVEQSEKEKASEKRTVASLREQVSNLKLQLQ
                     QLGEVIQEKEKGSSLISQQSKQIIALQEIVEDQKRQLEELKIKIQELVSANQELGKQN
                     QSLSKENLHNKNTVEDLKKKLTELNVQLEQLHQSSSEKEQTIRKLREELIKKDSSLKQ
                     NEEMQLAQKHLQEEIDRLQKEIKQQQLNTNQLESIIAQSKEAEKRYQQALQQKKGIYL
                     ARMERVSPIYLQIQQIEQKAKELEERRETEASTAAKTLATKLRLEIKSYLDNNESDEK
                     SALNSFKINAKRHIENSKETLNQHREEWKYLLANVTLGVFLLGIGYLAAILINKATTG
                     NYTFFSQTNSGKKLDALEKAISSTHSETLVYG"
     gene            complement(7277..7513)
                     /locus_tag="U6309_001474"
     CDS             complement(7277..7513)
                     /locus_tag="U6309_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504922.1"
                     /translation="MLQPFITDFIKNRSFQFIFVMGLVADIALAGTIKHIVFLFGFPY
                     LSSVGLYCLMKRKKTTVKARISEQYRPKQKEICR"
     gene            complement(7549..8232)
                     /gene="radC"
                     /locus_tag="U6309_001475"
     CDS             complement(7549..8232)
                     /gene="radC"
                     /locus_tag="U6309_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEN5504923.1"
                     /translation="MMVAHTAQQLDLREKLLTNGAHSLSDIELLAVFISSGNNKKSCL
                     QLAYELTKHLGNLRNILNADLQSFKSIHGLGEVRYAQLQAAKEICHRSDFIDLQKEIR
                     LSNTQQTYAFLKKRLRDYKNETFAALFLDNQHRIIAYEELFSGTINTATVYPRPIVER
                     VLQLNAAALILAHNHPSGLSDASQQDLAITERIRDALDLVDARLLDHIVIGDNEVYSI
                     FAENKWVCN"
     gene            8309..9505
                     /gene="coaBC"
                     /locus_tag="U6309_001476"
     CDS             8309..9505
                     /gene="coaBC"
                     /locus_tag="U6309_001476"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEN5504924.1"
                     /translation="MQDFINKKILLGICGGIAAYKSAHLVRELTRLGADVRVVMTHSA
                     QQFINPLLMQALSGNEVRIDLFDTQAERAMGHIELARWADYLLIAPASANCLAKMAQG
                     LADDLLSTLYLVTETPVIVCPAMNRSMWAHPATKANCELLKERGVFFVGPEEGSQACG
                     EHGLGRVSEADQIVNTLRLLDVNQLLYGKRIVITAGPTRESIDPVRYLSNYSSGKMGF
                     AMAEAAAMAGAQVTLISGPCHLQTFSDIELIRVESAQSMFDEVNRHLQQGDLFIGTAA
                     VSDYSVLNPASEKMKKNENSELTLQLVRNTDILSEVAKSGKASFVVGFAAETTDVISY
                     AKEKMKNKKLDMIIANQVGKGMGFESDVNQVTVITKNKQIELPLTHKTRLAGQIIAIL
                     AASLQN"
     gene            9532..9990
                     /gene="dut"
                     /locus_tag="U6309_001477"
     CDS             9532..9990
                     /gene="dut"
                     /locus_tag="U6309_001477"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEN5504925.1"
                     /translation="MHQVIQLKILDPRIGDTIPLPAYATDGSAGLDLRVCISEPMQVA
                     PQQTVLLPTGIAIYIADPKLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGELKISCW
                     NRGQELFTVNPGDRIAQLVFIPVVQTSFEVVNEFTESSRGEGGFGSSGRY"
     gene            9991..11379
                     /locus_tag="U6309_001478"
     CDS             9991..11379
                     /locus_tag="U6309_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEN5504926.1"
                     /translation="MTYQQKQIPRSVFRAYDIRGIIGKELDEHSFYSIGLAIARYLQK
                     LNRRQIFVARDGRLTSYALATALKEGLLDSGIDILDLGAVATPVMYYATHTQGIDSGL
                     MVTGSHNPADYNGIKLVLAGKTLIQDEIDTLYALLGEVKQSAVRGKETAFDVIEDYIQ
                     RIVSDIQLKRPLKVVVDCGNGVAGPIIPRVISKLGCEVIPLYCEVDGRFPNHHPDPSI
                     EANLVDLKAAVANHQADIGLGFDGDADRLGLVTNKGEVIWPDRLMMFYSREILSRNPG
                     ATVVFDVKCSSHLEKEIQAAGGVARMCPTGHSIVKAVMKKEQAILAGEMSGHLFFKDR
                     WYGFDDALYSACRLLEIISSSHLTVSEQFELIPNSVNTPEIKIAITDEEKFDFIQRFS
                     EQADFPEGRILNIDGLRVEFPNGWGLLRASNTTPCLVARFEATDKDHLEQIQYLFKKQ
                     IQRLDSELDLPF"
     gene            complement(11485..13218)
                     /locus_tag="U6309_001479"
     CDS             complement(11485..13218)
                     /locus_tag="U6309_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN5504927.1"
                     /translation="MNSEVDTLFAQAYKLQYEGHLPQAISLYEQILAQSPKHTETLHF
                     LGLTYAQLGDMENAILYFLQARKINPNDVGILNNLANAYKKAGQLDEAIKYYQQAIEI
                     KPEYVQAHNNLAATYALLNNYQKALHHYVIAVNTEPDFSAAHFNLGLLLLKNQQLSAA
                     KTQFNNVIALNPKHREAQFYLGILHLEDNLLSEAEQAFHKVLEQDHEHVQSLINLGVI
                     ALKREQNQLAVDYFTKALALDNEDIDARNNLAATFMHHDRFENALMHYDVLLKKEPNN
                     LEYLYNSGVAQMALGHLNEATLLFDQILTLQSDHTPSLNNLAAIYLKMDMRETAREYL
                     ERALVINPDDVVSRHMLNAITGATSVNTTEKYAQNLFNNYALYYDQHMQGELHYKIPH
                     HIGRLIHQLQLLQTSHSLDLGCGTGLTGIVLREISKHLTGVDIAEKMIARAKEKNIYD
                     LLVCSELIDFLRKNKNDYDLAVAADVLPYFGNLDDIFNYINQHLKQEGYFIFTTEIST
                     TTPWKLESSARFSHQPEYIESLINKYQFHLIKQEKIPGRTQNKQVLEVMLYCVKKSTS
                     RFVSNRTYENP"
     gene            complement(13220..13804)
                     /gene="rdgB"
                     /locus_tag="U6309_001480"
     CDS             complement(13220..13804)
                     /gene="rdgB"
                     /locus_tag="U6309_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEN5504928.1"
                     /translation="MKEIILATSNPGKIKELEQLLAPTICIPQADLGISDAEETGLSF
                     IENAILKARHASSLANKPALADDSGLVVPSLNGEPGIYSARYAGIKANDEDNIQQLLS
                     KMADLSQEQRQAYFFCAIALMQHAKDPTPLIATGVFHGVISMKPSGTNGFGYDPVFYL
                     NEYQCTAAELPAKIKNRISHRAKALNQLRALLPD"
     gene            13853..14410
                     /locus_tag="U6309_001481"
     CDS             13853..14410
                     /locus_tag="U6309_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN5504929.1"
                     /translation="MRILEKNIKKTPWYLKPFFWNQKRKYGQILEPALIWARIPRLFL
                     AVSAMYGVIDRKKSPLSPILRSLITVRVSQINWCEFCVDINSATLIKRAGSEAHYKQL
                     AKWRSSSLFSPEEKAALDYAEKVTYSDQRPDDECFNQLKLYYSEQEIIELTALIAFQN
                     MSSKFNSALGVEAQGFCSRQLTGKS"
     gene            complement(14437..15783)
                     /gene="sdbB"
                     /locus_tag="U6309_001482"
     CDS             complement(14437..15783)
                     /gene="sdbB"
                     /locus_tag="U6309_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdbB"
                     /protein_id="HEN5504930.1"
                     /translation="MAKTITKDSFFHSRLQQFVANSLFPAGAGDWYKNKGYGKKGEVD
                     DEAPFRDFVEKQKTDKKSHYYKEFQGLDLSLKKVKTKLVSGESCQLEVMKCQPESNEP
                     KKPGTGKHIVYFPGANTYYQACFRDISTACKETGATVHAFNFPGTGLSSGKVREANDL
                     INAGLSVVSSLIKQGVHPDDIILQGDCYGASIALEVKRQLEEQADIKVRAIMNNVFKS
                     FKAAVCDMITQSPWLPNILKSIVKRLLEFTGWHVTPGKKYKHADPYQCHIQHLGDQTL
                     ESSTLSSKVSKYQHEIQTGQTKSQKRAPITDTCPEEYRKDRDELDRKHYVRVKETAKE
                     RLASKFGVDKFGRVNAHFADLCELEMLDGQSVYQGFVNDYIARSNAYIEKHPQKGIKD
                     IQQDLSQLHYLQAADSIEITEDEAQDFNTVVDLITDEQQLRHDRFNDNTIGKSISM"
BASE COUNT         4448 a         2878 c         3161 g         5409 t
ORIGIN      
        1 ttgtgacata aaagaaacat gagctatgct attaaattat ttaactaact caagaatgtc
       61 atttgaatat gagtattttt aagacaataa agaacattat atataaccct aatgtctaca
      121 ttgctgtggt aattggggca ctatttggag gggtaagtgg tggcgctgtc gggctatttt
      181 ccggtggatt cattggccga tcgtttaaaa tatgtatgga ctgtcctaat cagttattgg
      241 gttttaatat aggtattttt gatctcaata tggtagcggg tgctattatt ggagtggtta
      301 taggagctgc tttgggtggg gcgattactg gtttgattac tagctttcat gtttacagaa
      361 agcctcattt acctaagaca gtttctcgcg ataacataca tgaagtatta ataagtgcat
      421 tatggattag tattgaaata tccattggaa ttattttagg cgctgtcata ggaagtttaa
      481 actcaccggg tattgggtca gtgacaggcg ctctcatagg tataattctt atgttattaa
      541 ctaccatttg ggaaaataga gccaaaaaat aacaatgggg ctagctccat cgctttggag
      601 cctgaccctg actgaattaa ttattttgct ttttcaattt taattgtttt tacattttcc
      661 ttggcttcag tttttttagg tattgtgatc cataacatac cctttttaaa agaggcagtc
      721 gctttatcca catccgcaga tagaggtaag gaaatggtgc gttcatatcg cccataacta
      781 atttcacgac tgatataatt tttattttca tcttttttag aagtggtttt ctctccttca
      841 atggttaatc tgttttcaca aaaagatact ttaatatctt cttcgcccat accaggcatt
      901 tctacttcaa ttttgaaatt atctttatct tcaacgatat ccaaagacgg agtaagggaa
      961 atatgctcga attgctcaag attggcactt ctttgagggt caaataaatt ataaaaatca
     1021 ctcatagcat gattgacctc ttgctgaagt cttaaaaacg gactcattag aagatcagtt
     1081 gttttacctg gttgatgttt tcttaagctg cccatgatgt cacctcctta ttccataaat
     1141 tgacgtatca gttagttgtt ataaacaccg aaagtaatat tcaccagctt atcaatgtaa
     1201 atatagttgc tagataaaat attttcaatt gttataaatt tatttcataa tgattttata
     1261 tcgttgtttt tgtatttatt ttattctttt aaatattgct taaaccaatt ttgagcgagg
     1321 cgagctactt ccttgatgat ttgaatttgt tgtaatagct tttgataatt atagctttat
     1381 ttcataacaa taaccccggc tcctctatac ctgccatgtc ttaaatcatc cagtgctcga
     1441 ttaacttcag taaggggata ggtttgaact tttgtcttaa ctgggatttt aggggccaga
     1501 gacaggaact cttctccatc tttgcgagtt agattagcaa ttgaacaaac agtacgttca
     1561 ccccatagta tttcataagg aaagctggga atatccgaca tatgtatacc agcacaaacc
     1621 acagtacctc cttttacagt attgtgaaga gcctgaggca ctaatgcacc aacgggtgca
     1681 aaaatgattg ctgcatcaag aagatgaggt ggtgattgcg tagaatctcc ggcccagaca
     1741 gcacctaatc ggtatgcaaa tttttgagtt tcctgatcac ctgggcttgt aaacgcataa
     1801 atttttattc ctttttggca agctacttga atcagtatat gtgctgccgc tccaaatcca
     1861 tataggccta aatgagttgc gttaccagcc tttagcaagg cacgatatcc aattaaacca
     1921 gcacaaaata atggtgctgc ctgatgatcc gggtatccat cgggaatagg aaagcaaaaa
     1981 tggtaatttg ctacacagaa ctcagcgaaa ccaccatcta tttgataccc ggtaaagcag
     2041 gcttgatcac ataaattttc tcgaccagag gtgcaaaaat tacagatccc gcaacttcca
     2101 cctagccaag ggactccaac tcgctgtcca attgcaaatt tctttacagc ggatccaacc
     2161 tgctcaacag ttcctacaat ttggtggcct ggaataaggg gagtcttggg atttttcagc
     2221 tcaccatcga caacatgcaa gtcagttcta caaatcccac aagctgatac tttaattaaa
     2281 agttcatttt catttggatt tggtttattg acttcctgat attgcaatgc acatccaggc
     2341 ttttccatga tcatagcatg catgggtatt ggtcctgttt ttatcttcta tcttacaatt
     2401 atagtagggt gggttatact tacttaatgc attcaaaaag taacaatatt tgacatcaat
     2461 gctgatgaat gaatagcttg cctggaggcc attaatcggg gtcgttgaca ggcagcaaaa
     2521 aattgaaaaa cagcagcgat cttgataatt aatttggaaa aagcgtttgt ttttcggaat
     2581 ttattttgtg gattgcaata tttttccaat aggcaactca tattatgaac gctatacgtg
     2641 ctttttttgc tatttcacta ccaccagcga tccaggaaaa tatccggagt aatttggagc
     2701 cattgaggca aatggcacca gttcactcca taaggtggac aaagccaatg catttgcata
     2761 tcaccttgca atttttacag agactggaaa caaaacatat tcagatcttg attgaaagag
     2821 taagtaatga acttaaccat ataaagcaat ttgatttgga attcggtgtt ttagagtggt
     2881 tcccttctcc caaaaatcca agacttattt cattatctgt aggtccacaa gatagactaa
     2941 gggatttgtc tcgaatgatc ggtcaggcga taggtggttt aaattatcct gtagaaaaac
     3001 gtcctttcag agggcatttg actgtaggta gagtagtgaa tttcagccca aaacttcagc
     3061 agttgttgca aataaaattt cctatcattc caccagttcc gattaataaa ttttatttaa
     3121 ttgaaagtag gcctgataaa gaaggaagta attattttcc cttggcacaa tttgatttaa
     3181 cccggtaaaa gagaatggct gatttgccaa ttggaacgat tctttgcctc tgtgtccaac
     3241 ttattatgct caagtgtctt taattatttg gcataggatg aatttaagcc aacctatgcc
     3301 aaaaagagaa gattatccat aaactaatgt ttcggagtga gtggaagaaa tcgctttttc
     3361 caacgcgtcc aattttttcc cgctgttagt ttggctgaaa aaggtataat tgccagtagt
     3421 tgctttgtta atcaaaatag cggcgagata accgattcca agcaagaata cccctaaagt
     3481 aacattggct aacaggtact tccattcttc gcgatgttga tttagcgttt ccttcgagtt
     3541 ttcaatatgc cgtttggcat ttattttgaa tgagtttaag gcactttttt catcggactc
     3601 attattgtcg agataacttt ttatctctaa acgaagtttt gttgcgagtg tctttgccgc
     3661 tgttgatgct tctgtttccc ttctttcctc caattctttg gccttttgtt caatttgctg
     3721 tatttgcaaa taaataggag acactctttc cattcgtgca aggtaaattc cctttttctg
     3781 ttgtaaagct tgctgatagc gtttttcagc ctctttgctt tgagcaatta tagattctaa
     3841 ttgatttgta tttaattgtt gttgtttaat ttctttttgt agacgatcaa tttcctcttg
     3901 cagatgtttt tgggctaatt gcatttcctc attttgtttg agcgaagaat ctttcttaat
     3961 gagttcttct ctcaattttc taatggtttg ttctttttca cttgaggatt gatgcaattg
     4021 ttccaattga acatttaatt cggtcaattt tttcttaaga tcttcaactg tattcttatt
     4081 atgtaaattt tccttgctca aactttgatt ttgttttcca agctcttgat ttgcagatac
     4141 aagttcctga atttttattt tcaattcttc caattgtctt ttttgatctt ctacgatctc
     4201 ttgaagagca ataatttgtt tggattgctg gcttatcaaa gaggagcctt tttctttttc
     4261 ctgaattacc tctccaagtt gctgtaattg caattttaaa ttgcttactt gctccctaag
     4321 tgaagccaca gttcgttttt cggaagcttt ctctttctca ctttgctcga ctcttttttc
     4381 tttttcttgt aattgcttct caaattctct aatttgtaat tgtttctctt caagttgttc
     4441 gctaagagag tggacttgtt ttgttaattt aagttgattt tcttcgtttt ttcgagttaa
     4501 ttcatgcatc tcttttcgaa gcgattcaag agcagaattg gccaatgatt tttctttagt
     4561 gattctttca atagtttctt cctggtgtgt gttgctttcc ttttgttttt gtaactcctc
     4621 tttcaagctc tctacaagta atgtttgctc ttgaacttgt tcgcttaaag attttaactg
     4681 caattgtaat gcttcttgtt tttgcagttc taatttaagc tgattaagct ctgaggttag
     4741 agattcaata gcagatttat ccagggattt ttcatgttga ctttgtttgt cagctttttt
     4801 caggactaga ttttcctttt ctttctctgc taattgctct tctaactgtt gtacttgtaa
     4861 ttgtttttct tcgagcgttt tatgaagaga gctttctttt tttgccaatt catgcagctc
     4921 ttttcgaagc gactcaaggg aagaatcggc caatgatttt tctttggtga ttctttgaat
     4981 ggtttcttcc tggtgcgtgt tggttttctt ttgcgtttgc agcttctctc ctaatcgctt
     5041 ggatagagtc gtttgttcat gtattttctc tttgagttct tttaactgga actgtaattt
     5101 ttcttgctct tgcagttgta atttaagctg actcagttct aaattcagag agcgaataat
     5161 ggattgggca gaagatttct catgttcgct ttgctcagcc atcttctctt gtaactgata
     5221 tttttccttt atttggatta aagtcgcttc taactcctca aattcaatcg tttgcttttg
     5281 aatttttgat tgaagagaaa ttactatctt ttgcagctca tcaatttgtc cagcagtttt
     5341 tttaccagaa atctgtaata attgatgcca ttcacggatt ttagctattt ttccggcggg
     5401 atcatctgtg cggcctttaa atacaccact ttcaaaagca gagtttatac gtttccaaaa
     5461 ttttttatta ctgagaattc tttcaattaa aggcgacggt ttagggctat ttagttcctc
     5521 cagacaaatc gttgcaagtt gatatccatt cattttagct aatgcttttt catcaatggg
     5581 ataaaaaaac tcactttcct tatcaggcaa gggctctgca aaagtaggtg cttttaatga
     5641 ttcctttaca tcagcttctg caagttgaaa agcaatgaat tgttctttca atgcttttaa
     5701 attctctgta actgcctcat ctattccgaa tgcttgagcc tgtttgatta tttttagcgt
     5761 attaataatt ccattaccta aagtcagtaa ggtttggatt ttaacccagg cagagtcttt
     5821 atgttccttg caatagagtt gacatggtcc ttcaaaagcg gcaaaacgag ataagttgaa
     5881 agtattaggg tctactgtaa ataggcggcg caatacaatg ctttctttgt agtaaggaac
     5941 aaaggcatgt ccgtgaatcg cttcaccacc actattatcg tagagagcaa acagattagg
     6001 ggtgctgtct attctttctt ttgcccagac attattggct tcacacgtga tagctagttc
     6061 atcaacgtaa agattaaatt gtttgactaa ttccagtagg ttgattggat tactatcaaa
     6121 cgaaaatttt tccatgtctg aagtttttaa gatgtttgtc cattgattta tggtttctga
     6181 aaaagaggtt gaagaggtca taggaagcgt catgtatttt aattccaaaa tactttgata
     6241 cagttcttca ggattgctca atggggacat atctttaaga acagcaattt ttcctaaacg
     6301 attttctaat aatgtggcca tggcaatggc aaaatctttg caattacctt ttgggccaaa
     6361 tgttgcttct ttaaatgaat ccatacacat gtaggcggca agttggattt tggttttttc
     6421 ttctatcaga tcagctggaa tattttggag tgcgctagtt actatttcaa taagcaattc
     6481 aggatttagt ttggattgca attgtcgggc tttttcagcc atcgcaggga agaggccatt
     6541 gatcttctta tagttcagga aataatcttt agttagtgat ttataattaa accaacctct
     6601 gttttcataa gcataaagga gattatcatt attgtttgga tgagcgaaat aacggaaggc
     6661 atctccccaa tcgattcggc caaattgttt gcttttttct tctccttctc ctttgagtac
     6721 aacgatattg ccactatgta cactatgggc tcccagcaat agagaaaaca taagagccat
     6781 agaaaggcca aaatatttat tttgttttgt gatcccggca taataacctg gcccaaagaa
     6841 ggtttccttt atcgtattac gatcactgcc atcaccgtag cttgttccaa tgattttgtg
     6901 taattcatca aatgaaacca gtggctgtat caggccatag gatttgtctt caaattgaat
     6961 gacatcggcg caaatcaatg atggaaaata gctttcatca atcaatcctc gcttcatgaa
     7021 ctcttttaat agcaagcctg caaataattc cgtgaatagc tcacgagggt cgcccggttt
     7081 ttttataaaa aatttccggc catcgctgtc tttgtaaaaa ccatcgacat cacttttatt
     7141 tttaccacct gttttttctt taaatcgaac tatttcttta ccttgataag ttaacataat
     7201 gactctgacc taaaacaaca ctttgtgcca ttatagccac caagtattaa ctaaacctta
     7261 agaaaacctt aagaattcat ctgcaaattt ctttctgctt tggccggtat tgctcactga
     7321 ttcttgcttt aacagttgtt tttttacgct tcattagaca ataaagacca acagagctta
     7381 aataggggaa cccaaagaga aatactatgt gctttatcgt cccagccagg gcgatatcag
     7441 caactaatcc cattacaaaa ataaattgaa acgaacgatt ttttataaaa tctgtaataa
     7501 aaggttggag catttttaat ccattaactc aaatgatagg gacgggaact agttacatac
     7561 ccatttgttc tcagcaaaaa tggaatacac ttcattatcc ccaatgacaa tgtgatccag
     7621 taaccgggca tctaccaggt ccagagcatc tcgaatgcgt tctgtaatgg ctaaatcttg
     7681 ctggcttgca tcagacaagc ccgagggatg attatgtgcc agaattaaag cagcagcatt
     7741 taattgcaat acacgttcta caatcggtct gggatagact gttgctgtgt tgatggttcc
     7801 tgagaataat tcttcataag caatgattcg gtgttggtta tccagaaaga gtgcggcaaa
     7861 agtttcattt ttgtagtctc gcagtcgttt ttttaaaaaa gcataagttt gttgcgtgtt
     7921 agataaccgg atttcttttt gcagatcaat gaaatcacta cgatgacata tttctttcgc
     7981 agcctgaagt tgtgcataac gaacctctcc taatccatgg attgacttaa agctttgcag
     8041 atcagcattg aggatattac gtaaatttcc caagtgtttg gtgagctcat aggctagttg
     8101 caaacaagat tttttattgt tacccgaact gatgaaaacg gctaacaatt cgatatcaga
     8161 gaggctatgt gctccattag taagtaattt ttcgcgcaag tcaagttgct gcgctgtatg
     8221 ggcaaccatc attttgtatt ccttttatat tcctttattg cgaagtttgt gctttgatcg
     8281 gagttattct tatcaaaaat ggcgagccat gcaagatttt attaataaaa aaattctact
     8341 tggtatttgt ggcggcattg ctgcttataa atctgctcat ctggttagag aattgactcg
     8401 tttgggggct gatgtgaggg ttgtcatgac tcattcagca caacagttca ttaacccttt
     8461 attgatgcag gcattatcag gcaatgaagt gaggattgat ttattcgata ctcaagctga
     8521 acgagctatg ggacatattg aattagccag atgggcagat tatttgctca ttgctcctgc
     8581 gtctgcaaat tgtcttgcta aaatggctca aggtcttgct gatgatttgc tttcaacatt
     8641 atatctcgtt actgaaacgc cagttattgt ttgtcctgcc atgaacagaa gcatgtgggc
     8701 acatccagca accaaggcaa attgtgaact gttaaaagaa cgaggtgtgt tttttgtcgg
     8761 gcctgaagaa ggctcccagg cctgtgggga acatggattg ggaagagtca gtgaggcgga
     8821 tcaaattgtg aataccttgc gcttactgga tgtgaatcaa ctattgtatg gcaaacgaat
     8881 tgtgattact gcaggcccta ctcgcgagtc gattgatcca gttcgttatt taagcaatta
     8941 cagttcaggg aagatgggtt ttgcaatggc tgaagccgcc gctatggcag gcgcacaagt
     9001 gacattaatt agtggtcctt gtcatttaca aacgttttcg gacatcgagc taataagagt
     9061 tgaatcggcg caatctatgt ttgacgaagt gaacaggcat ttacaacaag gagatttgtt
     9121 tattgggaca gcagcggtat cagattatag cgtgctgaat cctgcttcgg aaaaaatgaa
     9181 aaaaaatgag aattctgaac tcactctcca gcttgttaga aatacagata ttctttctga
     9241 agtagctaaa agtggtaaag cttcctttgt tgttgggttt gcagctgaaa caactgatgt
     9301 gatttcctat gcaaaagaaa aaatgaaaaa taaaaaactg gatatgatta tcgccaatca
     9361 ggtaggtaag ggaatgggat tcgaaagtga tgtaaatcaa gtaacagtga ttacaaaaaa
     9421 caaacaaata gagctgccat taacccataa aacacgcctg gcaggccaaa ttattgcaat
     9481 ccttgctgca agtctgcaaa attaggcgca ataaaacgat tgagaattat tatgcatcaa
     9541 gttattcagt taaaaattct ggatccaagg ataggagata ccattccatt gcctgcctat
     9601 gccactgatg ggtccgccgg tctggattta agggtctgta tctcggagcc tatgcaagta
     9661 gcacctcagc aaacagtatt gctgcccacc ggcatagcta tttatattgc cgatccaaag
     9721 ctggcggcag tgattttgcc aagatcaggc ttaggccata aaaatggtat tgttttagga
     9781 aaccttgtag ggctgattga ctcggattat caaggagaat taaaaatctc ttgttggaac
     9841 aggggccagg aactttttac tgttaatcct ggtgatagaa ttgctcaatt agtttttatc
     9901 ccggttgtac agacttcttt tgaggtggtc aatgaattta cagaaagctc cagaggagaa
     9961 ggtggttttg gcagctccgg gagatattaa atgacctatc aacaaaagca aattccccgc
    10021 tctgtctttc gtgcctatga cattaggggg attatcggta aagaactgga tgagcattct
    10081 ttttatagca ttggtttagc cattgcacgt tatttacaaa aattaaatcg tcgacaaata
    10141 tttgtagctc gtgatggacg tctgaccagt tatgcattgg ctacggcctt gaaagagggt
    10201 ttgctagaca gcgggataga tatccttgat cttggtgctg tggcgacacc tgtcatgtat
    10261 tatgctaccc atacgcaagg gattgattct ggtctgatgg ttaccggaag tcataatccc
    10321 gctgattata atggtatcaa gttagtattg gctggaaaaa cattaattca agatgaaata
    10381 gatacacttt atgctttgct tggtgaagtc aagcagtcag ctgttcgtgg caaagaaacg
    10441 gcttttgatg tgatagaaga ctacattcag cgtatcgtga gcgatattca attgaagcgc
    10501 cctttaaaag tagttgttga ttgtggcaat ggtgtggcgg gtccaattat tcccagggtt
    10561 atttcgaaat tgggctgtga ggtcattccc ctgtattgtg aagtggatgg ccgatttccc
    10621 aatcatcatc ctgacccttc aatagaagcc aatttagttg atttaaaagc ggctgtggca
    10681 aatcatcaag ccgatattgg tttgggcttt gatggggatg ctgacagact tggcctggtg
    10741 acaaataaag gcgaagtgat ttggcctgat cgtttaatga tgttttactc aagagagatt
    10801 ttaagtcgta atccaggcgc tacggttgtg tttgatgtga agtgctccag tcatttggag
    10861 aaagaaatcc aggcagcagg cggtgttgcc agaatgtgcc ctactggcca ttcgatcgtg
    10921 aaagcggtga tgaaaaaaga acaagctatc ttggctggcg aaatgagcgg gcatcttttc
    10981 tttaaagatc gctggtatgg atttgatgat gctttatata gcgcatgccg attgttagag
    11041 attatcagtt cttctcattt gactgtcagt gagcaatttg agttgatccc taatagtgtc
    11101 aatactcctg aaataaaaat agcgattact gatgaagaaa aatttgattt tattcaacgt
    11161 tttagtgagc aggctgattt ccctgagggc aggattttga atattgatgg tttgcgtgtt
    11221 gaatttccaa atggatgggg attgttacga gcatcaaaca caactccttg tctggtcgca
    11281 cgttttgagg cgacagataa agatcatctg gagcaaattc aatatttgtt taagaaacaa
    11341 attcagcgcc tggattctga gttggatttg ccattttagt gtttaggaag atgaaaaatt
    11401 caatatagtg ctattctgct cagagtttgt cagatttgga taggactaac gaaaaagccc
    11461 aaggttcatt ttttaataaa gagattaggg gttttcgtaa gttctgttgg aaacaaaacg
    11521 agatgttgac ttcttaacac aatacaacat gacttccagc acttgtttgt tttgtgttcg
    11581 gcctggtatt ttctcttgtt tgataagatg aaattggtat ttattgatta agctctctat
    11641 gtattcgggt tgatggctaa atcgagcgct gctttccagt ttccacggag ttgttgtgct
    11701 gatttcggta gtgaaaataa aatacccttc ttgcttcaga tgttgattga tgtaattaaa
    11761 aatatcatcc agattaccaa aatagggtag gacgtctgca gcgactgcta aatcgtaatc
    11821 gtttttattt tttcttaaaa aatcgattaa ttccgagcat accagaagat catagatgtt
    11881 tttttccttt gctctagcta tcattttttc tgctatatct actccggtta agtgtttgct
    11941 tatttcacga agcacaatac ctgttaatcc agtaccacaa cccaagtcca aagaatgact
    12001 ggtttgcaaa agttgaagtt ggtggatgag gcggccaatg tgatgcggaa tcttatagtg
    12061 taattcacct tgcatgtgtt ggtcgtaata caaggcataa ttattaaata agttttgggc
    12121 atatttttct gtagtattaa cgctcgttgc tcctgtaatg gcattcagca tatgcctgct
    12181 gaccacatca tcggggttga ttaccagagc gcgctcaaga tattcgcgcg cggtttctcg
    12241 catgtccatt tttaaataaa tagcggccag attattcaag gatggggtgt gatcactttg
    12301 taatgttaaa atctgatcga acaaaagggt ggcttcattg agatggccta atgccatttg
    12361 agcgactccg gaattgtata aatactccag attattgggt tcttttttca gtaaaacatc
    12421 gtaatgcatg agcgcatttt caaatcgatc atgatgcatg aatgtggctg ccaaattatt
    12481 acgtgcatca atgtcttcat tatccaaagc caaggcttta gtaaaataat caacagctaa
    12541 ttggttttgt tctcttttca aagcgatcac gcccaagtta attagggatt gaacatgttc
    12601 atgatcttgc tctaacactt tatgaaatgc ctgttctgct tctgagagta aattgtcttc
    12661 caaatgcaaa atacctaagt aaaattgagc ttctctgtgc ttaggattta gagctatcac
    12721 attattaaat tgtgttttgg cagcagacaa ttgttgattt ttaagaagta acaaacctaa
    12781 attaaagtga gccgcgctaa agtcaggttc tgtattcact gcaatcacat aatgatgcaa
    12841 cgctttctgg taattgttga gtaatgcgta ggttgctgcc agattattat gagcctggac
    12901 atactctggt ttaatctcaa tggcttgctg gtagtattta attgcctcat ctaattgacc
    12961 tgccttttta taagcgttag cgagattatt gagtatacca acgtcattgg ggttaatttt
    13021 tctggcttgc aaaaaatata aaatggcatt ttccatatca cccaattggg cataggttaa
    13081 tcccaggaaa tgcagagttt ccgtatgttt gggtgattgg gcaagtattt gctcatataa
    13141 gctaatggct tgaggtaaat ggccttcata ttgaagctta taggcttgtg caaaaagagt
    13201 atctacttca ctgttcatat taatctggaa gaagggcgcg taattggttt aaagcttttg
    13261 cgcgatggct aatcctattt ttaattttag caggtaattc agcggctgtg cattgatatt
    13321 cgttgagata aaataccggg tcatacccaa agccatttgt tcctgaaggt ttcatgctaa
    13381 taacgccatg aaatacgcct gtggcaatga gcggtgttgg atctttggca tgttgcatta
    13441 gcgctattgc gcaaaaaaaa taggcttgtc tttgttcttg ggataaatca gccattttac
    13501 ttagcaattg ttgaatattg tcttcgtcat ttgcctttat tccagcatag cgtgctgaat
    13561 agatacccgg ctctccattt aaagagggga ctacaagccc cgagtcgtcg gctaaagctg
    13621 gtttattggc taatgagctg gcatgacggg ctttgagaat ggcattctct ataaaactta
    13681 atcctgtttc ttctgcatcg gatataccca agtcggcttg gggaatgcat atggtggggg
    13741 ctagtaattg ttctaattct tttatttttc cagggttact ggttgccaaa ataatttctt
    13801 tcattcttat tccatgttat taattcagta ttcataaaaa ggataattat gcatgagaat
    13861 tttggaaaaa aatatcaaga agactccctg gtatttaaaa ccattcttct ggaatcaaaa
    13921 aagaaaatac ggccaaatac tcgaacccgc tttgatttgg gcaagaattc ccaggttatt
    13981 tttggcagtt tctgctatgt atggcgtgat agacagaaaa aaatcaccac tttccccaat
    14041 attacgctct ctcatcactg tcagggtttc tcaaattaac tggtgtgagt tttgtgttga
    14101 tattaattca gcaactttaa ttaaaagggc tggaagtgaa gcacattata aacaactggc
    14161 gaaatggagg agttcctcat tattttcacc agaagaaaaa gcggcattgg attatgctga
    14221 aaaagtaact tattctgatc aacgtcctga tgatgagtgt ttcaatcaat tgaaattgta
    14281 ttattctgaa caggaaatca ttgaattgac agcactgatt gcatttcaga atatgtccag
    14341 taaatttaat agtgcccttg gcgttgaagc acaaggattt tgttcgaggc aactgactgg
    14401 caaaagctga atagtcctgc cagtcagtac ttgcaattac atagagatgc ttttacctat
    14461 tgtattatcg ttgaatcgat catgccttag ttgttgttca tccgtaataa gatcaactac
    14521 cgtgttaaaa tcctgagcct catcttcagt aatctctatt gaatcggctg cttgtaagta
    14581 atgtaattga cttaaatcct gttgaatgtc tttaatacct ttttggggat gtttttctat
    14641 gtaagcattc gatctggcaa tatagtcatt gacaaaacct tgatataccg attgcccatc
    14701 caacatttcc agctcacaca aatcagcaaa gtgagcattg acccttccaa atttatcgac
    14761 tccaaatttg gaagcaaggc gttctttagc ggtttccttg actcgaacat aatgttttct
    14821 atccagctca tcacgatctt ttcgatactc ttccggacag gtatccgtaa tgggagccct
    14881 tttttgagat ttggtttgcc cagtttgtat ttcatgttga tacttgctga ctttacttga
    14941 taatgtcgaa gattctaatg tttgatctcc caagtgttga atatgacatt ggtaaggatc
    15001 agcatgttta tatttttttc ctggagtaac atgccagccg gtaaattcta acaatctttt
    15061 aacaatgctt tttaatatgt taggtagcca tggtgattgg gtaatcatat cgcaaacggc
    15121 tgctttaaat gacttgaaaa cattgttcat gatcgcacga actttgatgt ccgcttgctc
    15181 ttctaattgc ctttttacct ctaatgcaat gctagctccg tagcaatccc cctgtaaaat
    15241 gatgtcgtcc gggtgcaccc cttgtttaat taaagaggaa accacagaaa gaccagcatt
    15301 gattaagtca ttcgcctctc tcacctttcc tgaactgagg cctgtccctg ggaaattaaa
    15361 agcgtgtaca gttgctccag tttctttgca agccgtgctg atatcgcgaa aacaggcttg
    15421 gtagtaagta ttcgctccgg gaaaatagac aatatgtttc ccagtgccag gctttttagg
    15481 ctcatttgat tctggttggc atttcatgac ttctaactga catgattcac cagaaaccag
    15541 ctttgttttc actttcttca gacttaaatc caggccttga aactctttat aatagtgtga
    15601 ttttttatcg gttttttgtt tttcaacgaa atctctaaaa ggagcttcgt catcaacttc
    15661 gccctttttt ccgtagcctt tgtttttata ccaatcaccg gcccctgctg gaaaaaggga
    15721 attagctaca aattgttgta aacgactatg aaaaaaactg tcttttgtaa tagttttggc
    15781 catttaattc cccagtgtga gcaaaataaa gtcagtatat ttcttaaaat tagcataaat
    15841 ttagcagaaa aaacttaaga gagtattaat aaagtacatt tggatttgct aaaaac
//
LOCUS       DASLOE010000059        15797 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.059, whole genome shotgun sequence.
ACCESSION   DASLOE010000059 DASLOE010000000
VERSION     DASLOE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15797
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.059"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..580)
                     /locus_tag="U6309_001483"
     CDS             complement(<1..580)
                     /locus_tag="U6309_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HEN5504931.1"
                     /translation="MSNEFEPYNKEVKKIEFTTWKYQDSNDAEHSPSDEEELINELEV
                     VKQEAFQKGYADGFQQAQEEINAIKAELSKWLDLIQKPVQILDEQLTQEIIQTIIWLS
                     QHCVAVELSVNPDKLNDLLNKIKGELPSLTGKKSISMHPDDVRWIKEVFSESNIPGLH
                     ELLVADPALNRGDFYLQGEHSELDGRIQTRFTT"
     gene            complement(722..1711)
                     /gene="fliG"
                     /locus_tag="U6309_001484"
     CDS             complement(722..1711)
                     /gene="fliG"
                     /locus_tag="U6309_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEN5504932.1"
                     /translation="MDGIERAAILLLSMGEKNASEVLKHLEPRQVQKVGMAMSTMNSV
                     SKAKMQNVLIDFISAIEDQTSLTVDTEHYLRTVLIEALGEEKAIPFIDRILVSNKDSG
                     LNKLKWLDGRLIAEVIRNEHPQIIATILIHLDSEQSAEVVSYFSAEKRAEVLLRMCNI
                     DTVKPEAISELGHVIEKQLSGQTTGKSSSVGGIKTVADVINFLDGAMEEEVLERIKDW
                     DEELSEKIRDKMFVFENIKDMDDRSVQTLLREVTSDQLKLALKGTTETTKEKIYSNMS
                     KRAADLLRDDIEVQGPVRVSDVERAQRDILAIARNLAEDGKISLGAKGGDELI"
     gene            complement(1714..3348)
                     /gene="fliF"
                     /locus_tag="U6309_001485"
     CDS             complement(1714..3348)
                     /gene="fliF"
                     /locus_tag="U6309_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HEN5504933.1"
                     /translation="MALADNASVAATKFASLSIPRQIGVLFGLAASIAIGVAVVLWSR
                     DPSYVPLYSQLNAQDSSEILTILERNSIDFKIDKNNGLLMVPADELQSARLKLAAQGL
                     PRDTAIGHDLLGSSGGVFNTSQFMENARYRQALEAELARTISKFNNIKSARVHLAIPR
                     ESAFVRDSQEPSASVFIDVYSGFEIKKHTIAAIVNLVASSIPNLSAGRVTVVDQDGQL
                     LNEGSGYNLFSETERFLDYRQNLEHQYAQKIQDILTPILGYGRVRAKVSADIDFTSYE
                     QTQELFNPELSALRSEQTMQESRNASNEASGVPGTLSNTPQPNSSLGQKNNPQQNATP
                     NNLPQQTSSDQMQAKDVRMQTTKNFELDKTISHTKNQPGTIKRLSVAVLVDNRPVMND
                     KTKKLEKKPLTKEEIEQIKLLVSDAIGLNVKRGDSLNVINSSFVKPDPIEAIPEEHFW
                     QKDSFWSVIKQIGGALFILAILFGVLRPMLKTLASNKDMEQATDNQDTGDFTLDGKIS
                     FRDVQDYETQLSLLRQVVDKEPKRVAQVVKSWVDRG"
     gene            complement(3521..3835)
                     /gene="fliE"
                     /locus_tag="U6309_001486"
     CDS             complement(3521..3835)
                     /gene="fliE"
                     /locus_tag="U6309_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEN5504934.1"
                     /translation="MSDINTVNLINQMKTLAAQAEGIKPDSGLNHSSFSSVFQQALNQ
                     VNELSQTADSLKTRFEMGDPNVSLGEVMIASQKSNLGFEATLRVRNKLVQAYQDIMNM
                     PV"
     gene            complement(3850..5163)
                     /gene="fleR"
                     /locus_tag="U6309_001487"
     CDS             complement(3850..5163)
                     /gene="fleR"
                     /locus_tag="U6309_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor FleR"
                     /protein_id="HEN5504935.1"
                     /translation="MSHVLIVEDDSVLREALAETMTISGHTYITAKDGKEALALLELH
                     NPSVVLTDIRMDKLDGNQLLNEIQKKRPGLPVILMTAYGSVEDAVTAIRNGAVDYLQK
                     PFSAQVLIEKIKQYIKTDLSEEDNDPIARDPKSQALLTMALRVAQSDVGVMISGESGT
                     GKEVLAHFIHDHSYRSKQPFIAINCAAIPEQMLEATLFGYEKGAFTGAYKSTPGKFEQ
                     AQGGTLLLDEVSEMSLGLQAKLLRVLQEKEVERIGSNKIINLDVRIIATTNRQLKEEV
                     QAGRFREDLYYRLNVFPLQWHPLRERTSDIIPLANYLIRKHCQHKQPIVPVISPDAQK
                     AMLSYSWPGNARELDNVIQRALVLQTQGIIEEEHLQITNSTTMPSEAKTKGTSKNLQI
                     HEYELIEKTLLENEGNRQKVAALLGVSERTLRYKLAKMREEGYVV"
     gene            complement(5187..6218)
                     /locus_tag="U6309_001488"
     CDS             complement(5187..6218)
                     /locus_tag="U6309_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN5504936.1"
                     /translation="MNDMHYSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVW
                     LNPSAEAMLGYGLIGSLWVDVIQKAFVPRADDGHEVSLADGRRVHVAISSLESLPGIL
                     ITLSDITASRDYEEAKENEKRLVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDH
                     RIKNWIHRLQECHASIEQQIQDLLLFARGTSIEPKYVDMEFWCSQLMQRAQPYIESYA
                     AVLDVNNHLIARESSIHGESLIGAILNLIINALQSEATQIDLTLASMDDSGIQISVED
                     NGNGMSEEVRAQAFSPFYTTKAQGTGLGLAVVFAVVKAHGGRVTLESTEGIGTQVTIY
                     LPCLNRNVG"
     gene            complement(6517..7821)
                     /locus_tag="U6309_001489"
     CDS             complement(6517..7821)
                     /locus_tag="U6309_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEN5504937.1"
                     /translation="MSLFNKEPDPPLAESLRPQHINEVIGQSHLLGEGKPLRLCFMGS
                     KLHSMILWGPPGVGKTTIARLTAQAFDCEWIALSAVFSGVKDIRAAIEKAQEYLIHDK
                     QTILFIDEIHRFNKAQQDALLPYTESGLITFIGATTENPSFEVNPALLSRAQVYVLKP
                     LSEQELKLLFLRAKQRALSSLQFTEEAMDFLISCADGDARRLLNLLEQTKTACLTIKT
                     AVVDVELLHNVIVQSGRRFDKGGEAFYDQISALHKSVRGSNPDAALYWLCRMLDGGVD
                     PYYLARRIIRMSWEDIGLADPKAIQIANDAAATYERLGSPEGELALAQAVIYLSVAAK
                     SNAGYVAFNQAMEFIKRDKSREVPIHLRNAPTQLMKKLGYGHEYRYAHDEPHGYAAGE
                     CYLPEGMPEVNWYNPVQRGLETKIAEKLAFLRQLDKDMKKKV"
     gene            complement(7837..8445)
                     /gene="lolA"
                     /locus_tag="U6309_001490"
     CDS             complement(7837..8445)
                     /gene="lolA"
                     /locus_tag="U6309_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEN5504938.1"
                     /translation="MNKLFLILLLIFSHEVFSQTSAEVLQSKLNAIQTMTANFSQIVK
                     AKNREVSRSSGSMALQRPGRFRWQTKDPLEQLIVADGQKMWIYDVDLEQVTVKNQEKG
                     LGGTAALFLSGYDETLTHDFDVSEKQNGKLTVFDLKSKSAKENFQRIKLIFSQSTLIG
                     LELYDQLGQITNVKLVQIKSNPKLPAKLFQFKPPKGVDVVKQ"
     gene            complement(8448..10832)
                     /locus_tag="U6309_001491"
     CDS             complement(8448..10832)
                     /locus_tag="U6309_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEN5504939.1"
                     /translation="MAKQHSGKQAGIPKKSMPSFIIKRLCEGSFILILTGALFVLLSL
                     FTYSTNDPGWSHASRSGMSVSNSGGQVGAYIADALYFAFGYFAFLLPIAFVYIAWAIL
                     KDFRSFKELDRMVLLLRTVGFILMVSGGCGLLSINHRFEAVDTIHSSGGIIGQTVGNG
                     WCQMLNMEGATLLLLAMFLVGTTWLTGLSWIKAIELIGYYTLNLFTFLDKFIRKGMQI
                     ISENFNKEKLKTPLIKTEQLPKPDNEKKKSVPKLFQDKKDKEQEKATPVLIASEEKPE
                     IVKSTNEFKEIRPPKTITPGTLPSLSLLDKGQPGKPMGGYTHEELESLSRDVEQHLLD
                     FGIQADVVAVHPGPVVTRFELQLAAGVKVSKLTALAKDLARSLSVISVRVVEVIPGKT
                     VVGLELPNHSRQVVRLSDVLSADVYQQAHSPLSLALGVDIGGHPMVVDLAKMPHLLVA
                     GTTGSGKSVGINAMILSILFKASPEQVRLIMVDPKMLELSVYDGIPHLLTPVVTDMKE
                     AASALRWCVEEMERRYRLMAALGVRNLAGYNTKITEAAANGQPLLNPLWKPVDSMDET
                     APELQALPYVVVVIDELADMMMVVGKKVEQLIARIAQKARAAGIHMILATQRPSVDVL
                     TGLIKSNIPTRISFQVSSKIDSRTILDQQGAEQLLGHGDMLYLAPGSGAPLRVHGAFV
                     DDKEVHRIADDWRSRGEPDYVDDILKMGNENGDGAFDDDNGGQSVEDDDPLYDQAVEF
                     VIQTRKASISAVQRRLKIGYNRAARMIEEMERTGIVGPLDGGYRDVLVTSVTED"
     gene            10905..11855
                     /gene="trxB"
                     /locus_tag="U6309_001492"
     CDS             10905..11855
                     /gene="trxB"
                     /locus_tag="U6309_001492"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEN5504940.1"
                     /translation="MSISNHHRLIILGSGPAGYTAAVYAARANLNPVLITGMQPGGQL
                     TTTTDVDNWPGDIEGLQGPALMERMQKHAERFDTQVIFDHIVKADLAQAPFTLQGDSA
                     TYTCDALIIATGASARYLGLPSETAYQGRGVSACATCDGFFYRNKSVCVIGGGNTAVE
                     EALYLSNLASSVTLIHRRDSLRAEKILQDKLFEKAHSGNIKIVWNSTLDEVLGDEKKV
                     TGAMIRNLKTGETDQLAMDGIFIAIGHTPNTAIFQDQLAMRDGYILIKSGLDGMATST
                     SVPGVFACGDVADHVYRQAITSAGFGCMAALDAEKYLDSI"
     gene            11858..12526
                     /locus_tag="U6309_001493"
     CDS             11858..12526
                     /locus_tag="U6309_001493"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEN5504941.1"
                     /translation="MAYDSNYTFPDPETSDKQGLLAIGGVLTPKRVLQAYSQGIFPWY
                     EPGNPVLWWSPNPRLILIPNEFKISRSLKKTLKKTFKLTVDTAFQRVISYCATCSDRT
                     NKTWITSEMIETYTQLHEMGYAHSFEIWDGSELVGGLYGISLGHAFFGESMFHTITDA
                     SKVALHFLCSIMQSWNFDFIDCQLPTLHLMRLGAKIISRKEFLHMLQETLKYPDKKGN
                     WSVD"
     gene            12590..12961
                     /locus_tag="U6309_001494"
     CDS             12590..12961
                     /locus_tag="U6309_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEN5504942.1"
                     /translation="MKQHSQGFLKLVAEAKKRINEISPQILKSKIDNNEKLIIIDVRE
                     TNEWETGKIPNAIHLSKGIIERDIEKIITDNQSNIVVYCSGGYRCALVADSLQKMGYT
                     NVFSLDTGLQGWLDAGYSLVK"
     gene            13092..13313
                     /gene="infA"
                     /locus_tag="U6309_001495"
     CDS             13092..13313
                     /gene="infA"
                     /locus_tag="U6309_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEN5504943.1"
                     /translation="MAKEDHIEMAGTVIDTLPNTMFRVELENGHIVTAHISGRMRKNY
                     IRILTGDKVKVELTPYDLSKGRIIFRDKG"
     gene            complement(13481..14839)
                     /gene="pmbA"
                     /locus_tag="U6309_001496"
     CDS             complement(13481..14839)
                     /gene="pmbA"
                     /locus_tag="U6309_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEN5504944.1"
                     /translation="MQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVAVNY
                     DRGFSVDVRMGEVETVAFSEDKGVGLTVYIGQRKGGASSTDTSPEALQSLVKAACEIA
                     KVSAEDPCFGLADKELMTKNHPDLDLYHSWDITPQQAIDLALKCESHALSLDKRITNS
                     DGVNVSSYESHHGYANTHGGEGFIHSTRHSISCSVIAKEGDEMQRDYDYTTARNAEHL
                     VSADLIAAKAVERALSRLGSKQIKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNS
                     FLLDSIGQQLFPEFVHIYEQPHLKGALGSAPFDGEGVPTRPNVLVEKGRLLQYVLGSY
                     SARKMGLKTTANSDGVHNLTIDPTCGDLGDLLKMMRKGLLVTELMGQGVNGITGDYSR
                     GASGYWVEDGVIQYPVDQITIAGNLKEMYRMILAVGSDINPNISMRCGSILIENMMVA
                     GK"
     gene            14885..15661
                     /locus_tag="U6309_001497"
     CDS             14885..15661
                     /locus_tag="U6309_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEN5504945.1"
                     /translation="MSSQTNVYPANKLNLWPLLILALAIVIPITSYSANHWQELTPGI
                     EYQDLEGGLLNPWSHIHVFRIDLNKNQMALVTAKNLAQKNASVDQFAEYSKALLSING
                     GFFDHEFNPLGLRINNKKQENPLKRISWWGIFYVKDNKPRITNIRNFHYDSNIDFAIQ
                     SGPRLLIRGNIPSLKAGVADRTALGITDDGKVIILVTTNAAMSTRQLAQIMRSPPLSC
                     SDAINLDGGSSSQLYAKIDSFQLNVHGFSNVSDAIIVKKI"
BASE COUNT         4642 a         3442 c         2730 g         4983 t
ORIGIN      
        1 aggtagtaaa acgagtttga attcgaccat ctaactcact gtgttcacct tgtaaataaa
       61 aatctcctcg atttaatgct ggatcagcca ccaataactc atgaagaccg gggatattac
      121 tttcagaaaa tacttcctta atccatcgca catcatcagg atgcatgctt atagacttct
      181 tgccagttaa agaaggtaac tcacctttta ttttattcaa taaatcattt agcttatctg
      241 gattaacaga aagctcaaca gcaacacagt gttgacttaa ccaaatgatg gtttgaatga
      301 tttcctgcgt caattgctca tctaaaattt gaacgggttt ttgaattaaa tccagccatt
      361 tggaaagctc tgctttaatt gcattaattt cttcttgtgc ttgttgaaat ccatctgcat
      421 aacctttttg aaaagcttcc tgcttcacta cctccaattc attaatcaac tcttcttcat
      481 cagacggcga atgttcagca tcgttggaat cctgatactt ccaagtggta aattcaattt
      541 tttttacctc tttattatac ggctcaaatt catttgacat agttatccaa ccttatcccg
      601 ggctcaaatc ataccaaaaa ttaccactta ggacaaataa aatacaattc tgatacgaac
      661 tcttgcttaa tgatttttac tattttcata aaatgtctaa aactcattct aggaaaagta
      721 gctagattaa ttcgtctccc ccctttgctc ccaggctaat cttgccatct tcagccagat
      781 ttcttgctat ggctaaaata tctcgttggg cacgttccac atcactgact cttacaggtc
      841 cttgcacctc gatatcatct ctgaggagat cagcagctcg cttagacata ttcgaataga
      901 ttttttcttt tgttgtttca gtagtaccct tcaatgctaa ttttaattga tcagatgtca
      961 cctctcgcaa taaagtttgt acacttctat catccatgtc tttgatgttt tcaaaaacaa
     1021 acatcttatc tcttatcttt tcgcttaact cctcatccca atccttaatc ctctccagta
     1081 cctcttcttc catagcgcca tccaaaaagt taatcacatc tgctacggtt ttaattccgc
     1141 cgacagaaga tgatttacct gtggtttgac cacttaactg cttttcaatc acatgtccaa
     1201 gttcagaaat tgcttcaggt tttactgtat caatattaca cattcgtaat aacacttctg
     1261 ctcttttttc tgcgctaaag taactaacca cttcagcaga ctgctcactg tccagatgaa
     1321 ttaatatagt ggcaatgatt tggggatgtt cattacgaat cacttcagct atcagacgtc
     1381 catccaacca tttcagttta ttcaaaccac tgtctttatt tgataccaat attctatcaa
     1441 taaatggaat tgccttttct tcacccaatg cttctatgag aacggttctg aggtaatgct
     1501 ctgtatctac ggttaagctg gtttgatctt cgattgcgct gataaagtca atcagaacat
     1561 tctgcatttt cgccttactg acactattca tcgttgacat agccattcca actttttgta
     1621 cttgcctggg ctctaaatgc tttaatactt ctgaagcatt tttttctccc atacttaaca
     1681 ataaaatagc tgctcgctct attccgtcca taattacccc cgatcaaccc atgatttcac
     1741 tacttgtgcg acgcgtttgg gctctttatc aaccacttga cgaagtaaac tcagctgagt
     1801 ttcataatcc tgaacatctc taaatgatat ttttccatct aatgtaaaat ctcctgtatc
     1861 ctgattatca gtggcttgct ccatgtcttt attgcttgcc aatgttttta acataggtct
     1921 taatacacca aataggattg ccaatataaa aagagccccg ccaatctgct taatcacaga
     1981 ccaaaaactg tctttttgcc aaaaatgttc ttctggaatt gcttctatag gatctggctt
     2041 tacaaaactg ctgttgatca cattcaagct atctcccctc ttaacattca atcctatggc
     2101 atcagaaacc aataacttta tttgttcaat ttcttcttta gttagcggtt tcttctccag
     2161 tttcttggtt ttatcattca ttacaggtcg attatctacc aataccgcaa ccgacaagcg
     2221 ttttattgtt cctggctgat ttttagtatg acttattgtt ttgtccaatt cgaaattttt
     2281 agtggtttgc atgcgaacat cctttgcctg catttgatct gacgatgttt gttgaggtaa
     2341 gttatttggc gttgcatttt gttgaggatt atttttttgc ccaagagaac tatttggttg
     2401 aggagtattt gataatgtcc caggaacccc gcttgcttca tttgacgcat tacgactctc
     2461 ttgcatagtt tgttcgctgc gtaaagcgga gagctccgga ttgaacaatt cttgagtttg
     2521 ttcatagctg gtaaagtcaa tatctgcaga tactttggcc ctgacacggc cgtatcccaa
     2581 tattggggtt aagatatcct gaattttttg agcgtattga tgttccaggt tttgtctgta
     2641 atcaagaaaa cgctcagtct ccgaaaaaag gttatatccg ctaccctcat ttagcaattg
     2701 tccgtcttga tcaactactg ttactcttcc tgcgcttaaa ttcggaatac ttgatgctac
     2761 taaattgaca attgctgcaa tggtatgttt ctttatttca aatcctgaat acacatcaat
     2821 aaatacagaa gcactaggtt cttgtgagtc tcttacaaag gctgactctc ttggaattgc
     2881 caagtgtact ctggccgatt taatattatt aaatttactg atcgtacgtg ctaattccgc
     2941 ctcaagagct tgtctatatc gggcgttttc cataaattgg ctggtattaa acaccccacc
     3001 actgctacct aaaagatcat gacctatggc agtatcgcga ggcaatcctt gtgcggccaa
     3061 tttcaaacgc gcgctttgca gctcatcagc aggcaccatc aacaagccat tattcttgtc
     3121 aattttgaaa tcaatactat tgcgctctaa aatagtcaaa atctctgatg agtcctgcgc
     3181 gttgagctga ctatataaag gaacatagct tggatcacga gaccatagaa ccactgccac
     3241 gcctattgca atactagccg ccaatccaaa caaaactcca atttggcgtg gtatagataa
     3301 gcttgcaaat tttgtagctg ctactgaagc attatcagcc aatgccatga tttactcctg
     3361 taatttttat ccttttttcc agaatacttc actatgagct ttaaaacttt actgagaaca
     3421 tttgcaaact cattatggta ttcaaaaacc ctataaaaac ctgctattga gtttgatcat
     3481 gtttatctgt tgaaaaagtt ctactaccca aaacattgta ctaaactggc atgttcataa
     3541 tatcctggta tgcttgtacc aatttgtttc taactcttaa tgtcgcctca aaccctaaat
     3601 tggatttttg acttgctatc attacttctc ccaaggacac attaggatcg cccatttcaa
     3661 agcgggtctt taaactatct gctgtttgtg ataattcatt gacttgattt aatgcctgtt
     3721 gaaaaaccga actgaaagaa gagtgattta aaccagaatc cggttttatc ccctcagcct
     3781 gggcagccag agttttcatt tgattaatta aatttactgt attaatatcg ctcattctct
     3841 cagcccctgt taaaccacat atccttcttc acgcattttt gcgagtttat agcgtaaagt
     3901 cctttcgcta acccccaata acgcagcaac tttttgtctg tttccttcat tttctaataa
     3961 tgttttttct attaattcat attcatgtat ctgtaaattt ttacttgtac cctttgtctt
     4021 cgcttccgaa ggcatggtag ttgaattcgt aatctgcaaa tgctcttctt cgatgatacc
     4081 ttgagtctgt aacaccagag ctctttgtat aacgttatcc agctctctcg cattccctgg
     4141 ccatgaatag gacaacattg ccttttgcgc atccgggcta atgactggaa ctattggttg
     4201 cttatgctga caatgtttac ggattaaata attcgctaat ggaataatat cacttgttct
     4261 ctctcttagt gggtgccact gcagaggaaa aacattcaat cgatagtaaa ggtcctctct
     4321 aaaacgccct gcttgaacct cttcctttaa ttgcctgttt gtcgtcgcaa taatacgcac
     4381 atcaaggttt attattttgt ttgaaccaat cctttctact tctttttcct gtaaaactct
     4441 aagtaatttt gcctgcaaac ctaaggacat ttcactcact tcatctaaaa gtaaagttcc
     4501 gccctgggct tgctcaaact tgccaggggt cgatttgtat gctccggtga aagcaccttt
     4561 ttcataacca aacagggttg cttccagcat ttgttcagga attgcagcac aattaattgc
     4621 aataaaaggt tgtttgcttc gataagaatg atcatggata aaatgtgcga gcacttcctt
     4681 tccagtaccg ctttctccac tgatcattac tcccacatcg gattgtgcaa ctctcaaagc
     4741 cattgtcagt aaggcttggc ttttaggatc ccgtgcaata ggatcattat cctcttcact
     4801 cagatcagtt tttatatact gctttatttt ttcaattaag acttgcgcgc tgaagggctt
     4861 ttgcaaataa tcaacagcgc cattgcgaat cgctgtaacc gcatcctcta cagaaccata
     4921 agctgtcatt agaataaccg gcaaacctgg cctctttttt tgtatttcat ttaataactg
     4981 atttccatct aatttatcca ttcttatgtc agtgagtacg acacttggat tatgtaattc
     5041 aagaagtgct aatgcctctt taccgtcttt agcagtgata taggtatggc ctgagatcgt
     5101 catagtctca gctaacgctt ctcttaatac tgaatcatct tcaactatta aaacatgact
     5161 catagaaaat cctttgtttg ataaagttac cctacgtttc tgttcagaca aggtaaataa
     5221 atagttacct gtgttcctat cccttcagtc gactcaagag tcactcgacc accatgcgct
     5281 tttactacag caaaaaccac tgctaatcca aggccggtac cttgtgcttt tgttgtatag
     5341 aatggcgaaa atgcttgcgc cctgacttct tcagacattc cgtttccatt atcctctact
     5401 gatatttgta tacctgaatc atccatggat gcaagtgtta aatcaatttg ggttgcctca
     5461 gattgcagcg cattgattat cagatttaaa attgctccaa tcaaagactc tccatgtatg
     5521 gaggattcac gagcaataag atggttatta acatctaata cagctgcata agactcgatg
     5581 tatggctgag ctctttgcat taattgagag caccaaaact ccatatcgac atactttggc
     5641 tctatggaag ttcctctggc aaataataat aaatcctgaa tttgttgctc tatactggca
     5701 tgacattctt gcaatctgtg aatccagttt ttaatgcgat gatccaaact tgggagatta
     5761 tttaaatgct cggtatacaa tatggcagaa gacaaaggag ttctaatctg atgagctaat
     5821 tgtgcggtca ttgtaccaat agaaaccaat cgtttttcat tttctttggc ttcttcataa
     5881 tccctgcttg cagtaatgtc agacaaagta atcaatatac cgggtaaact ctctaaagac
     5941 gaaatggcta cgtgcacacg ccttccatca gccagagaca cctcatgccc atcatcagct
     6001 cttggaacaa atgctttttg aatcacatct acccatagtg aaccaatcag tccataacca
     6061 agcatcgctt cagctgaagg atttaaccac accactttgc cttgggcatt caaaattacc
     6121 aaggcggtag gcaaactgga caaaatgtcc cattcagata attctatctg tggtttgact
     6181 gcacaagttt gataatttga ataatgcatg tcattcattt taattattat tatgaaatgt
     6241 ctctagcaaa gttcaggcca aaattaaaat gtatttgaaa tcaattaatt aaccaattga
     6301 aaaccagatt tttttaaatg tatttaaaaa tataaacaat ttgtaatttt taaatcattg
     6361 taaatggggt taaggagatt ttaaagattc atccttgctt cctacagtca gaattttgac
     6421 atgttcttta tacttctccg gatgccaatg tcgattggac ttcttctttg ggataaattg
     6481 ggagacaaac ctaacctggc ttaaacagag aagaaatcaa actttctttt tcatatcttt
     6541 atctaattgg cgcagaaaag cgagcttttc tgcaatttta gtttctaaac ctctttgtac
     6601 cgggttatac caatttactt ctggcattcc ttcaggtaaa taacattcac cagccgcata
     6661 gccgtgtggt tcatcatgag catatcgata ttcatgacca taacctagtt ttttcattaa
     6721 ttgagtggga gcgtttctca agtgaatggg tacttctctc gacttgtctc tttttataaa
     6781 ttccatggct tgattaaatg cgacatatcc cgcattgctc ttggcagcaa cagacaaata
     6841 aattacagct tgtgccaaag caagctcgcc ctcgggtgag ccaagcctct cataagttgc
     6901 agcagcatca ttagcgattt gaattgcttt cggatctgct aacccaatgt cttcccatga
     6961 cattctaata atacgtctgg ctaaataata tggatccaca ccaccatcca gcattcgaca
     7021 tagccaatat aatgccgcat caggattaga gcctcgtacc gacttatgta aagcagaaat
     7081 ctgatcgtaa aacgcttctc cacctttatc aaaacgtcga ccactttgta ctatcacatt
     7141 atgtaaaagc tctacatcaa caactgctgt tttaattgtt aggcaggctg tttttgtttg
     7201 ctcaagtaaa ttgagtaatc gtctggcatc tccatcagca caagaaatta aaaaatccat
     7261 ggcttcctcg gtaaattgca gagaagataa cgctctttgc ttagccctaa gaaataagag
     7321 ttttaactcc tgctccgata acggttttaa aacataaact tgcgcccgtg ataataacgc
     7381 tggattaacc tcaaaagagg gattttctgt agttgcccca atgaaagtaa ttaaacctga
     7441 ttctgtataa ggtaataagg catcttgctg agctttatta aatctatgaa tttcatcaat
     7501 aaacaagata gtctgtttat catgaattaa atattcctgc gctttctcaa tcgcagctcg
     7561 aatatcctta acacccgaaa acacagctga taatgctatc cattcacaat caaaagcctg
     7621 tgcggttaat cttgctatag tagtcttacc gacccctggt ggcccccata agatcataga
     7681 atgcaatttg gagcccataa aacaaagtct taggggttta ccctctccaa gcaaatgact
     7741 ttgacctatg acctcattga tatgctgagg ccttaaactt tcagccaatg gtggatcagg
     7801 ttctttattg aataggctca taaatcaaca ttaaaattat tgttttacca catccacacc
     7861 tttagggggc ttgaattgga acaatttggc aggtaattta ggatttgact taatctgcac
     7921 taatttaaca ttagtaattt ggcccagctg atcatataac tccagaccaa tcaaagtact
     7981 ttgactaaaa attaacttaa ttctttgaaa attttctttt gcagatttgg atttcaaatc
     8041 aaaaacggtc aatttcccgt tttgcttttc actgacatcg aaatcatggg taagggtttc
     8101 atcatagccg ctcaaaaaca atgcagcagt accacctagt cccttttcct gatttttgac
     8161 tgtaacttgt tcaaggtcca catcataaat ccacattttt tggccgtcag caacaattaa
     8221 ttgttccaat ggatcttttg tttgccagcg aaatcgtccg ggacgttgca atgccatgga
     8281 acctgaagaa cgtgacactt cacgattctt tgcctttaca atttgactaa aatttgcagt
     8341 catggtttgt atagcattta atttactctg cagtacttca gcagaagttt gactaaacac
     8401 ttcatgcgaa aaaatgagca acaaaatgag aaacaattta ttcatattca gtcctcagtc
     8461 actgatgtta ctaatacgtc acgataacca ccatccaatg gaccgactat tcctgtcctc
     8521 tccatttcct ctatcattct ggcagcgcga ttataaccga tttttaatct tctctgtacc
     8581 gcagaaatac tagccttgcg tgtctgaatt acaaactcta ccgcctgatc ataaagagga
     8641 tcatcgtctt ctacggattg ccctccattg tcatcatcga aagcaccatc cccattttca
     8701 tttcccattt taaggatgtc atcaacataa tcaggttcac cccttgaccg ccagtcatca
     8761 gcgatacgat gaacctcctt atcatcgaca aaagcaccgt gcacacgcaa aggagctcca
     8821 cttccaggcg ccagatataa catatcacca tggccgagca attgttcggc tccttgttgg
     8881 tcaagtatag tgcgtgagtc aatttttgaa gacacctgaa atgatattct tgttggaata
     8941 ttggatttaa ttaaacctgt taatacatcc accgaaggtc tttgtgtcgc aagaatcata
     9001 tggattcctg ctgctcttgc tttttgcgca atacgagcaa tcaattgctc cactttcttg
     9061 ccaacaacca tcatcatgtc agcaagttca tcaattacaa caactacata aggcaatgct
     9121 tgcaactctg gtgctgtttc atccatagag tcgacgggtt tccataatgg atttaacaat
     9181 ggctgaccat tagctgccgc ctccgtaatt ttggtgttat accccgccaa atttctaaca
     9241 cccaacgcgg ccattaacct ataacgcctt tccatttcct ctacacacca acgcagtgcg
     9301 ctcgctgcct ccttcatgtc ggtaacaaca ggagtcagca aatgtggaat cccatcataa
     9361 actgataatt ccaacatttt cggatcgacc ataattaaac gcacttgctc tggacttgct
     9421 ttaaaaagaa tacttaaaat catggcatta ataccaactg atttaccaga accagttgtt
     9481 cctgcaacca gtagatgagg catttttgcc aaatcaacca ccatagggtg cccaccaata
     9541 tcaactccca aggctaaaga taagggagaa tgagcttgct gatacacatc tgcagataaa
     9601 acatcagata gcctgaccac ttgacgtgaa tgattgggta attcaaggcc cacaacagtt
     9661 ttaccaggaa taacctctac aactcgaaca gaaataactg ataatgaacg ggccaaatcc
     9721 tttgccaaag cggtcagttt gctcactttg acccctgctg ccaattgcaa ttcaaatcgg
     9781 gttaccaccg gccctgggtg aacggctaca acatctgcct gaataccaaa atcaagcaag
     9841 tgttgctcta catctcttga aagactttcc aattcctcat gtgtataacc tcccatcggc
     9901 ttccctggtt gacctttatc taacaacgac agactgggaa gagttccagg ggttattgtt
     9961 ttaggaggac gaatttcctt gaactcattg gtagatttga caatttcagg tttttcttca
    10021 cttgcaatta aaacaggtgt tgctttttct tgctctttat ctttcttgtc ctggaataat
    10081 ttaggaacag atttcttttt ttcattatca ggtttaggga gttgttcggt cttgatcaat
    10141 ggtgttttta atttctcttt gttaaaattt tcagaaataa tttgcattcc ttttctgatg
    10201 aatttatcaa gaaatgtgaa aagatttaat gtgtaatacc cgattaactc aattgccttt
    10261 atccaggata atccagtcag ccaggttgtt cccacaagaa acattgctaa taaaagtaaa
    10321 gtagctcctt ccatgttaag catttgacac caaccatttc caactgtttg tcctataata
    10381 cctcctgagc tgtgaatggt atctactgct tcaaagcgat gatttatact caataagcca
    10441 caaccaccag aaaccatgag gataaaacca actgttctca gtaacagaac cattctatcc
    10501 agctctttaa aagagcgaaa atctttcaaa atggcccagg caatgtaaac aaatgctatt
    10561 ggtaataaga aagcaaaata tccaaatgca aaataaagcg catcggcaat atacgcacca
    10621 acttgtcccc ctgagtttga aacggacata ccagaacggg aggcatgaga ccagcctgga
    10681 tcattggttg aataggtgaa taaagacaag agaacaaaaa gagctcctgt caaaatcaat
    10741 ataaaactcc cctcacacaa gcgttttatt ataaagctcg gcatggattt tttagggatt
    10801 ccagcctgtt ttcccgaatg ttgttttgcc ataggtattt taatgttttt atttttgtca
    10861 taatatttgc catcttaaca caaaaaataa ggaaggcaga gacaatgagc ataagcaatc
    10921 accatcgctt aatcatactc ggttcaggtc ctgcaggtta cactgctgca gtctatgccg
    10981 caagagccaa tctgaatccc gttttgatca ccggaatgca gcctggtgga caattaacga
    11041 ccacgactga tgtagacaac tggcccggag atattgaagg attacaaggc cccgctctaa
    11101 tggagcgtat gcaaaaacac gcggaacgct ttgatactca agtcatattc gatcatattg
    11161 tcaaagctga tttagcgcaa gctcccttta cactacaagg ggatagtgct acttacacct
    11221 gcgatgcctt gattattgca accggagcct ctgctcgtta tttgggatta ccctcagaaa
    11281 cggcctacca ggggcgaggt gtctcagcat gtgctacttg cgatggattt ttttatcgca
    11341 ataaaagtgt ttgcgttatt ggaggaggca acactgccgt tgaagaagca ctttatttat
    11401 caaatttggc gtcttccgtt actctgattc acagacgaga cagcttgcgt gcagaaaaga
    11461 tattgcaaga taaactcttt gagaaagcgc attccggtaa catcaaaata gtatggaact
    11521 caacccttga cgaagtactg ggcgatgaga aaaaagttac aggagccatg attcgtaacc
    11581 ttaaaactgg tgaaactgat caactggcaa tggacggtat ttttatagcc attggacaca
    11641 caccaaatac tgcaatattt caagatcagc tggctatgag agacggttat atcttgatta
    11701 agtcaggact tgatggaatg gcaacaagca cctctgttcc tggagtattt gcttgtggcg
    11761 acgttgctga tcatgtttat cgtcaagcca ttacatcagc aggatttggg tgcatggcag
    11821 ccctggatgc tgaaaagtac ctggattcaa tataaaattg gcttacgata gcaactatac
    11881 ttttcctgac ccagaaacca gtgataaaca gggtttatta gcaattgggg gtgttttgac
    11941 ccccaagcga gttttgcaag cctactccca aggaattttc ccttggtatg agcccggtaa
    12001 ccctgttctt tggtggtcac ctaatccgcg cctgatctta atacctaacg aatttaaaat
    12061 atccagaagt ttaaaaaaaa ctctgaaaaa aacttttaaa ttgactgtcg atactgcttt
    12121 tcaacgagtc atttcatact gtgcaacttg ttcagacagg actaataaga cttggatcac
    12181 tagtgaaatg atagaaacat acacccaatt acatgagatg ggatatgcgc attcctttga
    12241 aatctgggac ggttccgaac ttgtaggtgg actttacgga atcagccttg gacatgcttt
    12301 ttttggtgaa tcgatgtttc atacaataac cgatgcctcc aaagtcgcct tgcatttttt
    12361 atgtagcatt atgcaatcat ggaattttga ttttattgat tgccagttgc caaccttaca
    12421 tttaatgcgc cttggcgcaa aaataataag tcgaaaagaa tttcttcata tgttacaaga
    12481 aactttaaaa taccctgaca agaagggaaa ttggtctgtt gattaaatta attaaatttt
    12541 tttatcaata accgtttatc ttttaaaact aaatagagtg agaataagca tgaagcaaca
    12601 ttcacaaggt tttttaaaac tggtagcaga agcaaaaaaa cgtataaacg agattagtcc
    12661 tcaaatttta aaaagtaaaa tcgataacaa tgaaaagtta attataatag atgttcgcga
    12721 aacaaatgaa tgggaaacag gtaaaatccc gaacgcaatt catttaagta aaggaattat
    12781 tgagcgtgac attgaaaaaa ttatcacaga taatcaaagc aatattgtag tttattgcag
    12841 cgggggttat cgctgcgctt tggtagctga tagtctacaa aaaatgggat ataccaatgt
    12901 attttctctg gatactggat tgcaaggatg gctagatgct ggatacagcc tggtaaaata
    12961 attgaatatc tcccaataat gagccttggg ctatatcttt gaacaatgta attgcaagac
    13021 tggattgcca ttttccgcaa tttagtgcac tatttcactt ttaaaaattt gatacatcat
    13081 ttgagagaca tatggcaaaa gaagatcaca ttgaaatggc tggtactgtc atagatacac
    13141 tacccaacac catgtttcgc gttgagttgg aaaatggaca tatcgttacg gctcacattt
    13201 caggtcggat gaggaaaaat tatatcagaa ttctgactgg ggataaggtt aaggtagagc
    13261 tgactcctta cgatctttca aagggtcgta tcatttttcg cgataaaggc taaatgcgtt
    13321 tcatccaatt tctatagctg ttctctgttt cattaaagaa aatctgccgt aatatagaaa
    13381 aagctggctg caagcagcca ggtatatcgc taaaaaatgt tccagagaac tatcaccaag
    13441 attacagagc aagtggttaa cctcgatggt ttggggtatt ttatttcccg gcaaccatca
    13501 tattttctat tagaatagag ccacagcgca tggaaatatt cggattaatg tcactgccta
    13561 ctgccaatat catccggtac atttctttaa gatttcctgc aatagtaatt tgatcaaccg
    13621 gatattgtat gaccccatcc tcaacccagt atccacttgc cccacgtgaa taatctccgg
    13681 taataccatt cacaccctgg cccattagtt ctgtaaccag caaccctttc cgcatcattt
    13741 tcaaaaggtc accaagatcc ccgcaagtag gatcaatggt taaattatga acgccatcac
    13801 tatttgctgt tgtctttaaa cccatttttc gcgccgaata actgccaaga acatattgaa
    13861 gcaaacgccc tttttcaact aatacattag gccgcgtagg cacaccttcc ccatcaaaag
    13921 gagcgctccc caaagcacct tttaaatgtg gctgttcgta aatatgaaca aactccggaa
    13981 ataattgttg tccaatggaa tcaagcaaaa aagaattttt cctgtataaa tttgaaccac
    14041 taatggcgct cacaaagcta cccaataatc cactcgaaac tcttgaagaa aaaataactg
    14101 gggtcatttg tgttttaatt tgttttgatc ccagacgact taatgctctt tcgacagctt
    14161 tagctgctat caaatcagct gagactaaat gttccgcatt tctggccgtc gtataatcat
    14221 agtctctttg catttcatca ccttctttgg cgattaccga gcaacttata ctatgtcgag
    14281 tactatgaat aaacccctct ccaccatgag tattggcata accgtgatga gattcatagg
    14341 aagaaacatt aacaccatct gaattagtaa tccgtttatc taaagacaag gcatgtgatt
    14401 cgcattttaa agccaaatct atagcttgtt gtggggtaat gtcccaggaa tgatacaaat
    14461 caagatctgg atgattcttt gtcatcaatt ccttatcagc taatccaaag caaggatctt
    14521 cagcactaac ctttgctatc tcacaggctg ctttaaccaa agattgtaat gcttcaggag
    14581 atgtgtctgt actgcttgct ccccctttgc gctgacctat gtatacagtt aaaccaactc
    14641 ctttatcttc gctaaaagcg actgtctcca cttcgcccat tctaacatcg actgaaaaac
    14701 ctcgatcata atttacagct actgctgcat cagtggcacc ttcttttttg gcaagttcta
    14761 agacatcttt tatcactcga tttaaatctg ttgttgactt atatacttca ctattgttat
    14821 tttgcggttt aatttgcata tgagattccg tggtgttgaa tgatgtacat aaggatacaa
    14881 taacatgtct tcgcaaacca atgtttatcc tgcaaacaag ttgaatttat ggcccttgct
    14941 tattttagca ctcgccattg tcatccctat cacgtcctac tctgccaatc attggcaaga
    15001 acttactcca ggtattgaat atcaggatct cgaaggaggc ttgttaaatc catggtctca
    15061 tattcatgtg tttcgtatcg atcttaataa aaatcagatg gcattggtga cagccaaaaa
    15121 ccttgctcaa aaaaatgcgt ccgttgatca attcgctgaa tacagcaagg ctttgcttag
    15181 cataaatggc ggattttttg atcatgaatt caatcctctg gggcttagga ttaacaacaa
    15241 aaaacaggaa aatccattaa aacgcattag ctggtggggc attttttatg ttaaagataa
    15301 taaacctcgt attactaaca tacgcaattt tcattatgac agtaacattg attttgctat
    15361 tcaaagcggc cccagattat taatcagagg aaacatccct tctcttaaag ccggggttgc
    15421 tgacagaaca gctttaggga tcacggacga tggcaaagtg attatattgg ttacaacaaa
    15481 tgccgccatg tcaaccagac aattagcgca aattatgagg tcccctccat tatcctgtag
    15541 tgacgcaatc aatttggatg gtggaagttc cagccaatta tatgccaaga ttgacagctt
    15601 tcagctcaat gtacatggtt tttctaatgt cagtgatgcg atcatagtta agaaaattta
    15661 gtatcattca aggcaagctc cttatcattg attggattca aactccttat agtgcttaat
    15721 tcatgcgatc caaccacgtg cagaatctcc ttttcaaatt ccaccttcgt tttcgcgaga
    15781 tccgtaatcc cgaatct
//
LOCUS       DASLOE010000060        15745 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.060, whole genome shotgun sequence.
ACCESSION   DASLOE010000060 DASLOE010000000
VERSION     DASLOE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15745
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.060"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1245)
                     /gene="ankH"
                     /locus_tag="U6309_001498"
     CDS             complement(<1..1245)
                     /gene="ankH"
                     /locus_tag="U6309_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkH/LegA3"
                     /protein_id="HEN5504946.1"
                     /translation="MSIANDIISHRMPDFDAYLRAGESLDDIDEYGFTPLIECAITRQ
                     IKIAEQLIARKVDINKPDVTGRTPLHWAVDNNDLDMTKLLLTYGADPNAYTRNGLCVL
                     VYPVLRGQDAIKQLLYHHGAKLDFALDFINAKLIGHRFELQGDVDIVNAKGEFIELDY
                     EGFILEFTVAVVKDSLRRFISSYSTRHLRDYFPYIHNIMDAFTDAAELLQYQHHPRLG
                     EQHFKRIAALLKAPMLILPAASRGHALCFVRYHQWWAKIDRGENSLQEGSVNIYRITR
                     PEALTINFIHDFLYKKQNRRYYHQMINQQLGLLPFAQMPISSQISGNCSWANVQAVVP
                     VAYSMQELTSVENFKPDVALSLYDEWVEWDKDRALDECIQRFYLASPERKASIAAILG
                     GILFQACDHANKRHLERAEKILN"
     gene            complement(1246..2019)
                     /locus_tag="U6309_001499"
     CDS             complement(1246..2019)
                     /locus_tag="U6309_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEN5504947.1"
                     /translation="MAKEYYLSDEIEEIVLASTVLSYSRPNLSSKRFNDLKNGKTLWE
                     AKLDLKGLNVESARKSLYQFIQTQIKNNKQCVLIIHGADNSQDKPPLMKNLVNRWLPQ
                     INEVLAFHSAKPKDGGSAAVYVLLGSVFHVELPTRLPSNRSESTFLVAETKAMERRRR
                     LAEQQGERRFASVKAREHPEEEIKVAPEGELQNNILQHPELNSQRFDGIDPNLNPEPP
                     LNTEARREFDNERREQDKEKQLRLGNMPKFTTAPTPRGP"
     gene            complement(2168..3190)
                     /locus_tag="U6309_001500"
     CDS             complement(2168..3190)
                     /locus_tag="U6309_001500"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEN5504948.1"
                     /translation="MSRHLNVAIVGATGAVGETFLTVLEERNFPIKSLYPLASSRSVG
                     KTVTFRDQELDVLDLAEFDFSKVDLALFSAGGAVSKEYAPKAVAAGCVVVDNTSCFRY
                     EDDIPLVVPEVNPHRIADYTKRGIIANPNCSTIQMVVALKPIYDAVGISRINVATYQS
                     VSGTGKKAISELVAQVGDLLNGRPANVQVYPQQIAFNVLPHIDQFEDNGYTREEMKMV
                     WETRKIMEDDSIMVNPTAVRVPVIYGHSEAVHLELKKPLTADDARALLAKAPGVTVVD
                     NLSKASYPTAIKNAVGHDDVFVGRIRQDISHPCGLNLWIVADNIRKGAATNAVQIAEI
                     LQKEFL"
     gene            complement(3300..4358)
                     /gene="aroC"
                     /locus_tag="U6309_001501"
     CDS             complement(3300..4358)
                     /gene="aroC"
                     /locus_tag="U6309_001501"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEN5504949.1"
                     /translation="MSGNTFGALFTVTTFGESHGPAIGCVVDGCPPGMSLTEADIQPF
                     LDKRKPGQSKYTTQRREEDKVQILSGVFDGKTTGAPIALLIQNTDQRSRDYEDIKNLF
                     RPGHADFTYHYKYGHRDYRGGGRSSARETAARVAAGAIARLYLKRYLNLDIIGYLQQM
                     GDLKLQFENENEINKNPFFCPNNKQIQELADYVDRLRRQGDSVGARVKILARGVPIGL
                     GDPVFDKLDATLAYAMMSINAVKGVEIGAGFNAVEQLGSHHRDQMTAKGFLSNHAGGI
                     LGGIATGQPIEVSIALKPTSSITTPGQTINTEGEEVTVVTKGRHDPCVGIRAVPIAEA
                     MMALVLMDHYLRHKAQCK"
     gene            complement(4370..5302)
                     /gene="prmB"
                     /locus_tag="U6309_001502"
     CDS             complement(4370..5302)
                     /gene="prmB"
                     /locus_tag="U6309_001502"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEN5504950.1"
                     /translation="MVMVDTLVSSYFSKETEELVTILDFLRFSLSCAENAQLFYGHGT
                     DNVWDDMRSLILRSLSLPYDVDPFLLNARLTTSERKHLCNQLDKRINQRVPVPYLIKE
                     AYFCDLPFYVDERVLIPRSPIGELINNQFSPWIEAERVHHVLDLCTGSGCIAIACCYA
                     FPEAQVDAVDISNQALAVATINCERHDVGDQLALIESDCFTALSGKQYDLIVSNPPYV
                     GKEEMQTLPDEYRHEPVLALETGNNGLAIIEKILKNAHAYLSEHGILVVEVGNSEQAL
                     CETYPMVPFTWLEMSNGGQGVFLLTKQQLEDYFK"
     gene            5467..6036
                     /locus_tag="U6309_001503"
     CDS             5467..6036
                     /locus_tag="U6309_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEN5504951.1"
                     /translation="MTDDFLSDEDKALFRHHMRSVKPLNEKTKRVKNPTQPTHEKKTT
                     TFKSQSVPKEEYFLSDFIRDTVQSNTILSFSGPSIPSQRFRALKNGQIPWEAKLDLHG
                     LKTEAARQALYDFIQTQSLNNKRCLLIVHGKGGHQGAPPVIKNLINRWLPQFSQVLAF
                     HSALPKDGGLGALYVLLKRNREKDEQGNY"
     gene            6109..6528
                     /locus_tag="U6309_001504"
     CDS             6109..6528
                     /locus_tag="U6309_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN5504952.1"
                     /translation="MEKLGQFIINHWQLWLAFVVVVFLIFINEILSQRKKAKEVSPQS
                     LVDLMNNGNAIVIDIRDKESFKNGHIINSMNVNPDDFEQQKMSKYKDKPIILVCARGL
                     QSPTLAMKIRNQGYQPLVLSGGIQAWQNADLPLVKGK"
     gene            6533..6787
                     /gene="grxC"
                     /locus_tag="U6309_001505"
     CDS             6533..6787
                     /gene="grxC"
                     /locus_tag="U6309_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEN5504953.1"
                     /translation="MNEVILYTTGYCPYCIKAKELLDRKKIIYTEIRVDLQPELREEM
                     IQKSGRRTVPQIFINGQAIGGCDDLYALEAQGTLNELLKK"
     gene            6797..7285
                     /gene="secB"
                     /locus_tag="U6309_001506"
     CDS             6797..7285
                     /gene="secB"
                     /locus_tag="U6309_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEN5504954.1"
                     /translation="MTEQLNTNQQNDEAQFMIQRIYIKDLSYETPNTPAVFQQQWEPE
                     LKLDLNTTTTQLDKNVYEVVLTVTTTVMNQKTTAFLVEVKQAGIFTIQGAAASQLDHL
                     LHSFCPSILFPYAREAITSQVIRGSFPQLVLAPINFDALYMQQLEEKQKATGAKDETV
                     SH"
     gene            7287..8276
                     /locus_tag="U6309_001507"
     CDS             7287..8276
                     /locus_tag="U6309_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN5504955.1"
                     /translation="MNKKTIAMLGAGSWGTAVAIHLAKIGHKTLLWSHNPQHVALMAE
                     QHSNPAYLPGIPFPENLIPSDNLIECVQSADYVIIAVPSHAFAEIINKIPKPTQGLAW
                     LTKGVDPASHQLLSQLVASRFGVDFPIAVISGPSFAKEVARFLPTALTLASNNTNYQK
                     KMHQLFHHDNIRVYLSDDLIGVQLCGAVKNILAIACGISDGLGYGANAKAALITRGLA
                     EMTRLGLGMGARQDTFLGLAGVGDLVLTCTDDQSRNRRFGLLLGREVPIPEAEHQIGQ
                     VVEGKHNAAQICAIANKNKVEMPICEQINALLHGIVHAQQAVNNLMSRPAKEE"
     gene            complement(8354..9193)
                     /locus_tag="U6309_001508"
     CDS             complement(8354..9193)
                     /locus_tag="U6309_001508"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEN5504956.1"
                     /translation="MTRRSNQLNSNKDLAIGVFDSGMGGLTVLRALRDKLPHESFVYL
                     GDTARLPYGTKSPDTVKQYAMQMAKILVERRIKALVIACNTATTAALPHLQSMLTDIP
                     VLGVVAPGAAAAVSATKNHRIAVLATETTIASRAYHDLIIKNLPEAVVSSRACSVLVA
                     LAEEGMVTNAVAREALKHYLDDFSDEDAVLLGCTHFPVFKPLLSSLLPKDVAIVDSAQ
                     ATAISLYELLQNQNLHNDNLEVPPRVNYLVTDSINRFQKVGEIFLGEQLSLSDIELID
                     AMG"
     gene            complement(9265..12765)
                     /locus_tag="U6309_001509"
     CDS             complement(9265..12765)
                     /locus_tag="U6309_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="interaptin"
                     /protein_id="HEN5504957.1"
                     /translation="MPINKQLYDVLSDLSKLAQEDEVLRNKALDDILKADPSKPDEFR
                     KAIKDNEDFWKKYPVPNFGHLMTHETELTGLIGFRQQTPIPGYFTSDKFLDSQDTIHS
                     FQKMHQLAAEQRVKLGLVKSDVDTLVAILKNNPEECRAYIESKKPGLGNFSEGNVHGW
                     LKEEYTPTIPPKAINKSTGVLSDEAIKRIKEQARDLLLLKLINSSENTQLLKDLRDAM
                     SKPEAERAANALGFPTEGNGVLFLSREVVDALEERVEKLEQEAAKAGFDSYVQSLSHD
                     ALLAKKDGLESTTAAGFKNSLDEPYKTYLPESEWERAQGVLGARYLQAVLSSGTQNLK
                     DALNAKDTNALIAELKKPALLGPHDYIDKAVTEENLGSLKKNMMKSFVNNIKDETNLK
                     ALDALKALDGAKNLDKFKEVLGKLGITPADWVKDTDLKDMKQWARARQFELEINRVSS
                     LGSGAHSKLMSTLTQLPVEKQREILAKPQQLRHLMNAYEPHVAEHYLGKNASGIAELL
                     TENKRLEGFRAIHNAEVARVLANFKPEITLNDKQVAAINQALTTANSNPNTYTQVTDY
                     KTLIDAIKTQSGSVNQKDFYNAFNLNDDGRAFTSSTPRKDEMSKQQQHNQHIYAEYNS
                     TSNSGNKKLLAVLLSIEKPVTFSKDIVNRFLRPLKDSETPQEYADRLFGENPTNPANK
                     KFKDDLLRELTPTLFNEIKNDLRKQELLDTNPANVMSAIKALSTELESIKGITGPIRT
                     NADKLKFINDIDPVHLYNPTFQGTARSKAAQMKERYEGLSRDCELVVDQLRRQVIALE
                     GHLKSLPKDGQFKAAGLTLEQKEEIKKLRTDLQAELDAVRKDLDFYKKIQGKLETIVK
                     EVDVAAKGKMHYYYNSEGIKRHPPVSRDQIPPLPNVPNPSLRSSTTATTGSNGRIQEF
                     LVGEKIPEGQIVVVDVSHKTAPKSGAPVETIGRYTQDNNVPDQVTSKKGEISKVPGSK
                     FEILQFPTQVPPPNPPSGDPLVEAKVNFSMAMAADILASLDSPPTKDKPIRLRGSNPE
                     ELEYLYTALVILGEKNPKFKFNRDAIEVNSAVFHPDNVKGRLWGFSSNSLYSQVFTNT
                     GLTEAQNIIQSKIKHMQQMTDEKFSPQKEREKVDSKVQEITDKQSKMKKELNPVHKTT
                     ERTIEQEGPAPESPSTGMRK"
     gene            12910..13212
                     /locus_tag="U6309_001510"
     CDS             12910..13212
                     /locus_tag="U6309_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504958.1"
                     /translation="MRIKIILCGLILTNLTYAQGCIVGGQASQPRYVCFDGRTLDLTE
                     TGLVWNAKRGCFISSVPCCNYNATHYAYHPNPAKIGGAYARCRHDYPFTLGQMQTH"
     gene            complement(13216..14508)
                     /locus_tag="U6309_001511"
     CDS             complement(13216..14508)
                     /locus_tag="U6309_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HEN5504959.1"
                     /translation="MSPSESGELSIIQELSQRIVEAQRQIRILDSIKWDESVKQEFFK
                     FKGKKLPSIDKAYYDGKPLPFKVKDKQEEFRIILRDAQNQLGQYSTVTRLIRRQCEEY
                     SRAAQMLSLRGTPSFSEMAMELYGSPDDVFYVGGPRMCEMGSLLFDVLKNLQVQLQSQ
                     ADVKRYTPIQAQEILQERLEKFFDKHPGRVSVMVSDDMIADASAGADSIKLSQNAMFS
                     DRDLRYLEVHEGWVHVGTTLNGALQPNCFFLSKGSPSSSVIQEGLAVITEVVTFSSYP
                     GRMLKITNRVIALDKVTQGADFIDIYRYFLECGLSEEDSYNQSVRIFRGSTPTGGPFT
                     KDLSYAKGFVLIYNYIRFAIGQKRVDTIPLLFTGKLVLDDLPLLGELKEREILTDPVY
                     LPPPFQDLAALSAWMSFSLYLNQFDLNEIQKNFRFLLV"
     gene            complement(14518..14748)
                     /locus_tag="U6309_001512"
     CDS             complement(14518..14748)
                     /locus_tag="U6309_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504960.1"
                     /translation="MKKLSFIVLAALALTACNSRYASNGENLYLQSRNGEALVVPPPL
                     TSANISNFYDLPQQTQDARVSIAPPVEDITTS"
     gene            complement(14763..15635)
                     /locus_tag="U6309_001513"
     CDS             complement(14763..15635)
                     /locus_tag="U6309_001513"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEN5504961.1"
                     /translation="MFSGSIVALVTPMRNDSVDVHHLRELVEFHIAKGTHALVAAGTT
                     GEAGTLSHSEKLLVIKTVIEQAKERVPVIAGTAMNATKDCIELTQQAMEYGAHAALIM
                     TPAYIKPTQEGLYLHYSHIAQSVAIPIILYNVPGRTACDMLPETVARLAKISNIIGIK
                     EATGQMTRLQQILRLCEGSIDVYSGDDLTAAQWLLAGAKGVISVTANVAAKLMAKMCD
                     LAMDDDQAGCLRIQEQLMPLHELLFVESNPIPVKWAMNKMGLIGGELRLPMTELSEKH
                     HQALEKVLKNLELI"
BASE COUNT         4623 a         3530 c         2885 g         4707 t
ORIGIN      
        1 attaagaatt ttctccgctc tttccagatg acgcttgtta gcgtgatcac aagcctgaaa
       61 caaaatgcct cccaaaattg cagcaatact tgccttgcgt tcaggactgg ctaaataaaa
      121 tcgctgtatg cattcatcaa gagctctgtc tttatcccac tcgacccact catcgtacag
      181 tgataaagcc acatcaggtt taaaattttc tacgctggtc agctcttgca tagaatacgc
      241 gactggtaca acagcttgca cattagccca cgaacaattt ccactaattt gagaactaat
      301 tggcatttga gcaaaaggaa gcagccccaa ttgttggtta atcatttgat gataatatcg
      361 tctgttttgt tttttatata aaaaatcatg gataaaattg attgtcagtg cttctggcct
      421 ggttattcgg taaatattaa cacttccttc ttgtaagcta ttttcacccc tgtcaatttt
      481 tgcccaccac tggtgataac ggacaaagca taaggcatgg ccacgactcg ccgcaggaag
      541 aatcagcata ggggctttta acaaagcagc gatacgtttg aaatgctgtt cgccaagtct
      601 gggatgatgt tgatactgca acagttctgc cgcatcagta aaagcatcca taatattatg
      661 aatatatgga aaataatccc ttaaatgccg agtcgaataa ctactgataa atctccgcag
      721 ggagtctttt acaacagcaa cagtgaattc caaaataaac ccttcataat caagttcgat
      781 aaattcacct ttcgcattga caatatccac atcaccttgc aattcaaatc gatgaccaat
      841 caattttgca ttaataaaat caagagcaaa atcaagcttc gcaccatgat gatataacaa
      901 ctgctttatt gcatcttggc cacgcaaaac cggataaacc aatacacaaa gaccattacg
      961 agtgtaggca ttaggatcag cgccgtaggt taataagagt ttggtcatat ccaaatcatt
     1021 attgtccact gcccaatgta atggagtgcg tcctgtgacg tctggcttgt tgatatccac
     1081 ttttcgagca attaactgct ctgctatttt aatttgtcgt gtaatggcac actctatgag
     1141 gggagtaaaa ccatattcat cgatatcatc caaagattcc cctgcacgca gataagcatc
     1201 aaaatcaggc attctatgac tgataatatc gtttgcaata ctcatttagg gcccccgggg
     1261 ggtaggtgct gtagtaaatt ttggcatatt gcctaagcgt aattgttttt ctttatcctg
     1321 ttctcttctc tcattatcaa actcccttct tgcttctgta ttaagcggcg gttcagggtt
     1381 taaattagga tctattccat caaaacgctg actattcaat tcagggtgtt gcaatatatt
     1441 gttttgcaac tccccctctg gagcaacctt gatttcttct tcagggtgtt cacgcgcttt
     1501 aacagaagca aaccttctct ccccctgctg ctcagccaaa cgtcttcgtc tttccattgc
     1561 ttttgtttca gcaaccaaga aagtactttc actgcgattg gaaggcaatc gtgtgggtaa
     1621 ctcaacatga aatactgagc ccaacaagac ataaacagct gcagaaccac catccttggg
     1681 tttggcgcta tgaaatgcca atacctcatt gatctgaggc aaccaccgat tcaccagatt
     1741 tttcattaaa ggcggtttgt cctgagaatt atccgcgcca tgaataatta gaacgcattg
     1801 tttgttattt tttatttgag tttggataaa ttgatataag ctcttacgag cagattcaac
     1861 gtttaaaccc tttaaatcca gtttcgcttc ccagagagtt tttccatttt ttaaatcatt
     1921 gaagcgtttt gatgataaat tgggtcgaga gtaagacaat acagtactcg cgagcactat
     1981 ttcttcgatc tcatcggata aataatattc tttcgccatc tttttcaaaa tttaacagtc
     2041 ataatagaag tataacttag gatataaaaa gtatggcatt ttttctaata taacctgcat
     2101 gaccttatgg gggcatacag gttttctatc aagatactgg acctatgcta ttgcgggagt
     2161 gggagcttca cagaaactct ttttgcagta tttcagcaat ttgtaccgcg ttagttgccg
     2221 cacctttgcg aatgttatcc gcgactatcc acaaatttag tccacaagga tgagaaatat
     2281 cctgccttat gcgccctaca aaaacatcat catgcccgac tgcattctta attgctgtgg
     2341 gataacttgc tttagaaaga ttatccacta cagtaacgcc gggtgccttt gccaaaagcg
     2401 cgcgagcatc gtcagccgtc aaaggctttt ttaattccag atgaaccgct tcagaatgcc
     2461 cataaataac aggaaccctg acggctgtag ggttaaccat aatgctgtca tcttccataa
     2521 tcttgcgggt ttcccagacc atcttcatct cttctcgggt ataaccattg tcttcgaact
     2581 gatcaatatg aggaagcaca ttaaaagcaa tttgctgagg ataaacttga acattagctg
     2641 gtctcccatt taaaagatcg cctacctgag caaccagctc gctgatggct tttttgccag
     2701 tcccggaaac agactggtaa gtcgccacat taatacgact aataccaaca gcgtcataga
     2761 ttggctttag agcaacgacc atctgaatgg tggagcaatt agggttagca ataatgcctc
     2821 ttttagtata atcagcgatt cgatgaggat tcacttcagg gacaacaaga ggaatatcat
     2881 cctcataacg aaaacaggag gtattatcaa ccaccacgca cccagcagcc acagcttttg
     2941 gcgcatactc ttttgaaaca gcgccgcctg cagagaataa agcaagatcg actttgctaa
     3001 aatcaaattc tgctaaatcc aaaacatcca attcctgatc tctaaaagtc actgtcttac
     3061 ctactgagcg agaactcgct aatgggtaaa gggacttaat tggaaagttt ctttcttcca
     3121 aaacggttaa aaaggtttct ccaacagcgc cggtggcacc tacgatagct acattcaaat
     3181 gtctgctcat tatgacctca tcttaccatc aattaacaat gtgctagtca agtacaattc
     3241 ctgcttgcga agcagaaaac ggactacgat atggctatat tttgaaacgt tataatttat
     3301 tatttacact gcgctttatg tcgtaaataa tgatccatta acaccaaagc catcattgcc
     3361 tctgcaattg gcacagccct tatccccacg cagggatcat gccgcccttt ggttactaca
     3421 gtaacctctt caccttcagt attaatggtc tggcctggag ttgtgatgct tgaagttggc
     3481 tttaaagcga tgctcacttc gatgggttgc cctgtcgcta tgcctcctaa aataccaccg
     3541 gcatgattac tcaagaatcc cttggcggtc atctgatctc tatgatgact tcctaactgc
     3601 tccactgcat taaaaccagc cccaatttca acacctttaa cagcattaat tgacatcatt
     3661 gcataagcaa gagtagcgtc cagtttatca aatacagggt ctcccaatcc tattggcact
     3721 ccccgtgcca gtatctttac ccgagcacca acggaatcac cctggcgtct taaacgatct
     3781 acataatcag ccaactcctg aatttgctta ttattaggac aaaaaaaagg atttttattg
     3841 atttcatttt cattttcaaa ctgtaacttc aaatcgccca tttgttgtaa ataaccaata
     3901 atatccaagt ttaaataacg ctttaaatac aatctggcaa tagcaccggc agcgactcgt
     3961 gcggctgtct cacgagcgga agagcggcct ccccctctat aatctctatg cccatattta
     4021 taatgatagg taaaatcagc atgccctgga cggaacaaat ttttaatgtc ttcataatcc
     4081 cgggaacgct gatcagtatt ctgtatcagc aatgcaatag gagctcctgt cgttttccca
     4141 tcaaatacac cagaaagaat ctgtacctta tcttcctctc gtctctgggt agtatatttg
     4201 gattgaccag gtttacgttt gtctaaaaaa ggttggatat ctgcttcagt taaagacatg
     4261 ccgggaggac aaccatcgac tacacatccg atagcaggac cgtggctttc accaaaggtt
     4321 gttacagtaa ataacgctcc aaaggtattc cctgacatgc cttactcgct tatttaaaat
     4381 agtcttctaa ttgctgtttt gttaataaaa acaccccttg cccgccgttg ctcatttcaa
     4441 gccaggtaaa aggaaccata ggataagtct cgcataaagc ctgttcacta ttacctactt
     4501 ccactaccaa aatcccatgt tcacttaaat aagcatgggc attctttaaa attttttcaa
     4561 taatagccaa gccattattt cctgtttcca atgccaaaac cggctcatga cggtattcat
     4621 ccggcagggt ctgcatctct tccttcccca cataaggagg attgctcacg atcaaatcat
     4681 attgtttacc tgacagtgcc gtaaaacaat ccgattcaat cagtgccaat tgatcgccta
     4741 catcatgacg ttcacaattg atagtagcga cagccaatgc ttgatttgaa atatccacag
     4801 catccacctg agcctcaggg aatgcataac aacaggcaat cgctatacaa ccactcccgg
     4861 tacataaatc caaaacatga tgcactcgtt ctgcttctat ccaaggggaa aattgattat
     4921 tgattaactc tcctattggt gatctgggta taagcactcg ttcgtcaaca taaaaaggta
     4981 aatcgcaaaa ataggcttcc ttaattaaat aaggtacggg taccctttga ttaattcgtt
     5041 tatccaattg attacaaaga tgtttgcgtt cactcgttgt taaacgtgca ttcaacaaaa
     5101 aagggtcaac atcgtatggt aaagacaagc ttctcaatat cagagatctc atgtcatccc
     5161 aaacgttgtc agttccgtgg ccataaaata attgcgcgtt ttctgcacaa gacaaactaa
     5221 accgtaaaaa atccagaata gtaaccagtt cttctgtttc cttgctaaaa taactactca
     5281 caagcgtatc aaccataacc atagaaaaag tacaaattgt acactaaatt aaaatctatg
     5341 tcctgtattc tttgttaagg ggtgcgattt aagacgcccc ttcaaatggc tcgattttca
     5401 cctaacctaa agaattactt caaattaagt tataattaaa tatattcttt tgatacctgt
     5461 taaaaaatga ctgatgattt cctgtctgat gaggataaag ctctatttcg tcatcatatg
     5521 cgttctgtaa aaccattaaa cgaaaaaaca aaacgcgtaa aaaaccccac gcaacccact
     5581 catgaaaaaa aaaccacaac tttcaagagt cagtccgtac ccaaagaaga atatttttta
     5641 tctgatttta ttcgtgacac tgttcaatcc aacactattt tatctttttc aggaccaagc
     5701 attcccagcc agagattccg tgcactaaaa aatggacaaa ttccatggga agccaagctg
     5761 gatttacacg gattaaaaac tgaagcagct cgccaggcgc tttatgattt tatccagact
     5821 cagtcactca ataataaacg ctgcttactt attgttcacg gcaaaggagg tcatcaaggc
     5881 gctcccccgg taataaaaaa cctaatcaat cgctggctgc cgcaatttag tcaagtcctg
     5941 gcattccaca gtgctcttcc aaaggatgga ggattgggag ctttgtacgt cttattaaaa
     6001 agaaatagag aaaaggatga acagggcaat tactagtaac actttacttg cgttaaagca
     6061 gtaatcttag gataattcgt caaattaaaa aatatcttga gtacagctat ggaaaaatta
     6121 ggtcagttca tcatcaatca ttggcaactc tggctcgcct ttgttgtggt tgtgttttta
     6181 attttcatta atgaaatact ctctcaaaga aagaaagcca aagaagtttc accgcaatcc
     6241 ctggttgatt taatgaacaa tggaaatgcc atcgtaatcg acatacgcga caaagaatca
     6301 ttcaaaaatg gtcatatcat taattcaatg aacgtaaatc ctgatgattt tgaacaacaa
     6361 aaaatgagca agtacaaaga taaacccatt atcctggtct gcgcacgagg cttacaatct
     6421 ccaaccctag ccatgaaaat ccgtaatcaa ggttaccaac ccctggtttt gagtggagga
     6481 attcaagcct ggcaaaatgc tgatcttcct ttagtgaagg gtaaatgaac agatgaatga
     6541 agtcatactc tacactacag gctattgtcc ttattgcatc aaagcaaaag agctactaga
     6601 tagaaaaaag attatctata ccgaaatccg cgtcgattta cagcctgaat tacgtgaaga
     6661 aatgatacaa aaaagtggcc ggcgtacagt gcctcaaatt ttcattaatg gccaggcaat
     6721 tggtggctgc gatgatttat atgccctgga agcccaggga acattaaatg aacttttaaa
     6781 aaaataggaa taaaacatga ctgaacaact gaataccaat cagcaaaatg atgaagctca
     6841 attcatgatt caacgaatct atatcaaaga tttatcctat gaaacaccaa atacaccggc
     6901 tgtttttcaa caacaatggg aaccagagct caaactggat ctcaatacaa cgactactca
     6961 actggataaa aacgtttatg aagtggtttt aactgttaca accactgtga tgaatcaaaa
     7021 aaccacagca tttttggttg aagtaaagca agcgggcatc tttactatcc aaggcgccgc
     7081 agcaagtcag ttggatcact tgcttcatag cttctgcccc agcatcctgt tcccctatgc
     7141 tcgtgaagca attacctcgc aagtcattcg tggcagcttc ccccaattag tactcgcacc
     7201 gattaatttt gatgctttat atatgcagca gctggaagaa aaacaaaaag caactggagc
     7261 taaagacgag actgtttctc actaagatga ataaaaaaac cattgccatg ttaggtgccg
     7321 gctcctgggg cactgctgtc gcaattcatt tagctaaaat agggcataaa acgctgctgt
     7381 ggagccataa tccacagcat gttgccctca tggctgaaca gcattcaaat ccagcttacc
     7441 ttcctggtat accttttcca gagaatttga tcccttctga taatttaatt gaatgcgttc
     7501 aatcagcaga ttatgtcatc atagcagttc cttctcatgc ctttgcggaa ataatcaata
     7561 aaatccctaa accaacccaa ggattggcgt ggcttacgaa gggtgttgat ccagcgagtc
     7621 atcaattatt aagccaactg gttgccagtc gatttggggt tgattttccc atagcagtta
     7681 tttcaggccc atcctttgcc aaagaagtgg ctcgcttcct gcctacagct ctcactttag
     7741 ctagtaacaa tacgaactat caaaaaaaaa tgcaccaact atttcatcac gacaacattc
     7801 gtgtctattt aagtgatgat ttaataggtg tgcaattatg tggcgctgtt aaaaatatct
     7861 tggcgatagc ctgtggaatc agtgatggct taggctatgg tgctaatgcc aaagcagcgc
     7921 ttattacccg tggacttgct gaaatgactc gtttgggatt aggcatggga gcaaggcaag
     7981 atacttttct aggcttggct ggtgtagggg acctggtatt gacttgtact gatgatcaat
     8041 cacgcaatcg ccgctttgga ttgctcttgg gccgtgaagt gcctattcct gaagccgaac
     8101 accaaattgg ccaagtagtt gaagggaaac acaatgccgc gcaaatttgc gctatagcca
     8161 ataaaaataa agtggaaatg ccaatttgtg agcaaattaa tgcgctattg catggtattg
     8221 ttcatgccca acaggctgtg aacaacttga tgagtcgtcc cgccaaggaa gaataacctt
     8281 tgcccttgca aaaaatcata ttaaattaag tttagtgcac caggacgcag gtgatacaga
     8341 tggattgacg acattaaccc atagcatcaa tcaattcaat atcactcaaa ctcaattgct
     8401 cacctaaaaa tatctcgccc accttctgga acctattaat agaatctgtt accaaatagt
     8461 taactctggg gggaacttcc aaattgtcat tgtgcagatt ttgattttgc aataattcat
     8521 aaagagaaat tgcagtcgcc tgagcagaat cgacaattgc gacatctttt ggcaataagc
     8581 tggatagcaa gggtttaaat actggaaaat gagtgcatcc caataacacc gcatcttcat
     8641 ccgaaaaatc gtcgagataa tgcttcaatg cctcacgagc aactgcatta gtcaccatac
     8701 cttcctctgc caaggcaact aaaacactgc atgcccggct gctgacaact gcctcaggca
     8761 aattctttat gatcaaatca tgataggctc tggaagcaat ggttgtttct gtagccagaa
     8821 cagcaatacg atgatttttg gtagctgaca ccgcagcggc tgcgccaggt gccaccacac
     8881 ccagaaccgg aatatcggtt aacatgctct gtaaatgggg caaggcagcc gtcgttgcgg
     8941 tattacaggc aatgactaaa gcttttatac ggcgctcaac cagaattttt gccatttgca
     9001 tagcgtattg tttgacagta tcgggacttt tcgtaccata aggtaagcgg gctgtatctc
     9061 ccaaataaac aaaagattca tgaggtaatt tatcccgcag agcccttaaa accgtaagcc
     9121 cccccatgcc tgaatcgaat acaccaatag ccaaatcctt gttagagttc aattgattac
     9181 tccttcttgt catttatttc ttggctctct tattaggcaa aatagcttcg ttctaccaaa
     9241 aatgagagcc aaagtcatta ttatttactt acgcatccct gtactcggac tttcaggtgc
     9301 tggtccttct tgttcaatgg ttctttcagt tgttttatgc accggattca gttctttctt
     9361 catcttgctt tgtttatcag taatctcttg tactttggaa tccacttttt ctctttcttt
     9421 ctgaggacta aatttctcat cagtcatttg ttgcatatgt tttattttag actgtatgat
     9481 attctgggcc tctgttaatc ctgtgttagt aaatacctga ctatataatg aattgctgct
     9541 aaatccccac aaccgtcctt tcacattatc cggatggaat actgcagaat tcacctcaat
     9601 cgcatcacgg ttaaacttga attttggatt cttttcacca aggatcacca gggcagtgta
     9661 caaatattcc agttcttcgg gattagagcc gcgtaatcgt atcggtttat ccttggttgg
     9721 tggactatcc agactcgcta aaatatcagc cgccatcgcc atggaaaaat tgactttagc
     9781 ttctaccaga ggatctccac ttggagggtt tggaggtgga acttgggtcg ggaattgtaa
     9841 aatctcaaat ttacttccgg gaaccttgct tatttctcct tttttgctcg ttacttgatc
     9901 aggaacattg ttatcttgag tgtaccgtcc tatcgtttcc acaggagcac cggatttcgg
     9961 tgctgtttta tgagatacat caacgacaac aatctgccct tccggtattt tttcaccaac
    10021 aagaaattct tgtatccttc cgttagaccc agtagtagcg gttgtactcg atctcaggga
    10081 aggattagga acattgggta atggcggtat ctgatcacgt gaaacagggg gatggcgttt
    10141 gataccctca gaattgtagt agtaatgcat cttccctttg gctgccacat ccacctcttt
    10201 tacaatggtc tctaactttc cctgtatttt tttataaaaa tccaaatcct tgcgcaccgc
    10261 atccaactca gcttgcaaat cagtacgcaa ttttttgatt tcctcttttt gttccaaggt
    10321 caagccagca gccttaaatt gaccatcttt gggcaagctc ttcaaatggc cttcgagagc
    10381 aatcacctga cgtcgtaatt gatcaacaac cagttcacaa tccctggata acccttcata
    10441 tctttctttc atctgagctg ctttcgatct ggcggtaccc tgaaaggttg ggttgtataa
    10501 atgaacagga tcaatatcat tgataaactt taatttatct gcgtttgttc ttattggtcc
    10561 tgtgattccc ttaatagatt ctaattcagt actcagagct ttgattgctg acatcacatt
    10621 agcaggattg gtatctaata gctcctgttt cctgagatca ttttttatct cgttaaataa
    10681 agttggtgta agttcccgca acaaatcgtc tttaaatttt ttatttgccg gattagttgg
    10741 attttctcca aataatctat cagcatactc ttgaggagtt tcgctatcct tcaaaggacg
    10801 taaaaaccga ttgactatat ctttggaaaa ggtgacaggt ttctctatac tcaacaggac
    10861 agctaataat tttttattac ctgaattaga agtactgttg tattcagcat atatatgttg
    10921 gttatgttgt tgctgcttac tcatctcatc ttttcgagga gttgagctgg taaaagctct
    10981 gccatcatcg tttaaattaa acgcgttata aaaatctttt tgatttactg agccagattg
    11041 agttttaatg gcatcaatca aggttttgta gtcggtgacc tgggtatagg tattggggtt
    11101 agaattggcg gtggttaatg cctgattaat agcagcaact tgcttatcat ttaaagtgat
    11161 ttcaggttta aaattagcta aaaccctggc tacctcggca ttgtgaatgg ccctaaatcc
    11221 ctcgagtctt ttattttctg tcaagagctc tgcaatcccg gaggcatttt ttccaaggta
    11281 atgttctgct acatgaggtt cataagcatt cattaaatgg cgtaattgtt gtggcttggc
    11341 caaaatctct ctttgttttt cgactggaag ctgggttaat gtgctcatta atttcgaatg
    11401 ggccccgctg cctaaagaag acactctatt tatttcaagt tcaaactggc gagctcttgc
    11461 ccattgcttc atatctttca aatcggtgtc tttcacccaa tccgctggag taatacctaa
    11521 cttcccgagt acctctttaa atttatccaa atttttagcc ccgtctaatg cttttaaagc
    11581 atccaaggcc tttaaatttg tttcatcctt gatattatta acaaagctct tcatcatatt
    11641 ttttttgagc gagcctaaat tttcttctgt caccgcttta tcaatgtagt catggggtcc
    11701 caaaagggca ggttttttta attcagcaat caatgcattg gtatctttag cattgagagc
    11761 atccttgaga ttttgggttc ctgatgataa aaccgcttga agataacgag ctcccaatac
    11821 tccttgggct ctttcccatt ccgactccgg taagtacgtt ttgtaaggtt catccaaact
    11881 gtttttaaac cctgcagctg tcgtactttc taacccgtct tttttggcta atagcgcatc
    11941 gtgagataat gattggacat aactatcaaa gcctgccttg gcagcttcct gttctagttt
    12001 ttcaaccctt tcttccagag catcaactac ttctctactc aaaaacaata ccccgtttcc
    12061 ttcagtagga aagcccaagg cattcgcagc acgttcagcc tcgggtttac tcatggcatc
    12121 acgcaaatcc tttaataatt gagtattttc agaactgtta atcaatttaa gcaataacaa
    12181 atcacgagct tgttctttaa ttcttttgat cgcttcatca gataaaacac cagtagattt
    12241 gttaatagct ttcggaggaa ttgtcggggt atattcttct tttaaccaac catgcacatt
    12301 cccttcacta aaattaccca aaccaggttt tttggattcg atataagccc tgcactcttc
    12361 aggattgttt tttaaaatag caactaaagt atcaacatca gatttcacca acccaagctt
    12421 aacacgttgc tcagcggcca attggtgcat cttttgaaaa ctatgaatag tatcttgtga
    12481 atctaaaaat ttatctgagg taaaataacc aggtataggc gtttgttgtc ggaatcctat
    12541 taaccctgtg agttcagttt catgggtcat taaatgacca aaattgggga ctgggtattt
    12601 tttccaaaaa tcttcattat ctttaatagc ttttctaaat tcatctggtt tgcttggatc
    12661 agcctttaaa atatcatcca aagctttatt ccgaagaact tcatcttcct gagctaactt
    12721 agagagatct gatagcacgt cataaagttg tttattgatt ggcataagat atgctcctcg
    12781 gaaatcataa tgtaactatt ttaagccaaa aaacttaagt tatccttaaa ttttgccgat
    12841 attaatacca cagatggaaa cctaagctag ctttataatt agaagtatag caaggataag
    12901 gtgaatagaa tgagaataaa aataattctt tgtgggttaa ttctaacaaa cctcacctat
    12961 gcccaaggct gtattgttgg tggtcaggcc tctcaaccca ggtatgtctg ttttgatggg
    13021 cgaacgttgg atctgactga aacaggcctt gtatggaatg ccaaacgggg atgttttatc
    13081 agctcagtac cttgctgtaa ctacaatgcc actcattatg cctatcaccc aaatccggcc
    13141 aaaataggcg gagcctatgc ccgttgcagg cacgactatc cgtttactct tggccaaatg
    13201 caaactcatt aatgtttaaa ccaacaaaaa gcgaaaattc ttttgaattt catttaaatc
    13261 aaactggttc aaatacagag agaaactcat ccacgcactt aatgcagcca aatcctggaa
    13321 aggaggtgga agatacactg gatccgttaa aatctcgcgt tcttttaatt cacctagcaa
    13381 aggtaaatca tccaaaacca gcttgccggt aaataatagt gggattgtgt cgactctttt
    13441 ctgccctata gcaaagcgta tgtaattgta aattaataca aatcccttgg cataggataa
    13501 atccttggta aatgggccac ctgtcggtgt gctgcctcta aatatacgta cggactggtt
    13561 atagctgtct tcttctgaca atccgcactc caggaaatag cgataaatat caataaaatc
    13621 tgccccttga gtcaccttat ccagggcaat cacccggttt gtaattttca acatccgacc
    13681 tggataagaa gaaaatgtca cgacttcagt aatgactgcc aagccctcct gaatcacgct
    13741 gctggaagga gaacctttag acaggaaaaa gcaattcggt tgcaaggctc cattgagagt
    13801 cgttcccaca tgaacccagc cctcatgaac ttccaaatag cgtaaatctc tatcgctaaa
    13861 catagcgttc tgacttaact tgatgctgtc agctccagca gaagcatcag caatcatatc
    13921 atcgctgacc ataacactca ctcggccagg atgcttgtca aaaaatttct ctaatcgctc
    13981 ctgtaaaatt tcctgagcct gtatcggagt gtaacgcttg acatcagcct gagactgcaa
    14041 ttggacctgg agattcttta atacatcaaa tagcagggaa cccatttcac acatacgcgg
    14101 tccacccaca taaaacacat cgtccgggct accatacaat tccatagcca tttcgctaaa
    14161 tgaaggggta ccccgcaagg ataacatctg ggctgcgcgg ctatactctt cgcattgacg
    14221 tctgattagg cgagttactg tggagtattg ccctaattgg ttctgtgcgt cacgcaaaat
    14281 gatacgaaac tcttcctgct tgtctttgac tttaaaaggc agtggcttac cgtcataata
    14341 agccttatca atagaaggaa gtttttttcc cttgaactta aaaaactcct gttttactga
    14401 ttcatcccat ttgatgctat caagaatcct gatttgacgc tgcgcctcca cgatacgctg
    14461 agacaactcc tgtattattg acaactctcc tgattctgat ggcgacatga tgaatcctta
    14521 tgaagttgtg atatcttcca ccggcggcgc tatactcacc cgcgcatcct gagtttgttg
    14581 aggtaaatca taaaaattac taatattggc actagtcaat ggaggtggta ctaccaaagc
    14641 ttcgccattt cggctctgta aatataaatt ttctccatta ctggcataac gagaattaca
    14701 tgctgttagt gccagtgctg ccaagactat aaaactcaat tttttcacaa caaaaccctc
    14761 agttatatca actctaaatt cttcaatacc ttttccaaag cctgatgatg tttttcagac
    14821 aactccgtca tcggtaatct taattcccct ccaatcaatc ccattttgtt catagcccat
    14881 tttactggga ttggattcga ctccacgaac aaaagttcat gtaaaggcat taattgctct
    14941 tgaatacgca aacaccctgc ttgatcatca tccattgcca aatcacacat ttttgccatc
    15001 aatttagcag ccacattagc cgtgactgaa ataacacctt tagccccggc caacaaccat
    15061 tgggcagctg tcaaatcatc gccactgtaa acatcaatgc tcccttcgca taaccgcaaa
    15121 atctgctgta aacgagtcat ctgtccggtt gcttccttta ttcctattat attcgaaatt
    15181 ttagccagtc ttgccaccgt ttcgggcaac atatcgcagg ctgttcttcc cggaacatta
    15241 tataaaataa tcggaatcgc tacggattgg gcaatatgac tgtagtgcaa atataatcct
    15301 tcttgagtag gtttgatata ggctggtgtc atgattaaag cagcatgcgc tccatattcc
    15361 atagcttgct gggttagttc aatacagtcc ttggtcgcat tcatagcagt acctgcaatc
    15421 acaggtactc tttcttttgc ttgttcaatg actgttttga tgaccagaag tttttcgcta
    15481 tgtgataaag taccagcctc gccagttgtg ccagccgcaa ccaaggcatg agttcccttg
    15541 gctatgtgaa attcaaccag ttctcgcaaa tgatgaacat cgacactatc atttcgcatg
    15601 ggggttacca aggctactat acttccactg aacatgttgc cctcaaaaat taatgatcca
    15661 tgatcgtata ttactggaaa acagacaccc gagtaaattt ttatataaca ctttagtcaa
    15721 ctggaatctc tatcagcaat catta
//
LOCUS       DASLOE010000061        15534 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.061, whole genome shotgun sequence.
ACCESSION   DASLOE010000061 DASLOE010000000
VERSION     DASLOE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15534
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.061"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            174..1199
                     /locus_tag="U6309_001514"
     CDS             174..1199
                     /locus_tag="U6309_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504962.1"
                     /translation="MGWTDRFWNAYRVVTNTVIEGAANSLNAAGSLACLLGGAGVSLS
                     YSMSETIKAGYFGSVGVAGTVNLDVTAVEFNYGFNQTIPFRRDLLEKSGGGSYALNDF
                     ANPGTIFMISSLAILSGTTLRTFGANLKKWQQNREDKYHFIAKYSTDLEPPSYKEYLY
                     VNAESFFNSLAIASFSSLLAKNVLDFSIKDLSKYHFTYPFNGTNITGSVKTPDYTGPV
                     TSEFFPLDFFVSQNTTIHTDYFNLIVNEKVKANAMTNVTYGGGVHLEPSHQSSAPLLA
                     ASATIGIGTYLAGTFFSKKILRERDERLQRSRSSSYSIVSGTFWDNKHDIEAKGDTKF
                     EHTHSLQ"
     gene            1261..2454
                     /locus_tag="U6309_001515"
     CDS             1261..2454
                     /locus_tag="U6309_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEN5504963.1"
                     /translation="MVFKSRLVGIILLCFSSITGWCLSQSHEEDRIIVSNCLYEQLKD
                     KARLIAQSAHFSFIAIPIDENIANTLQKMRPVCGNYLNAEPYIQQTYNRFLDSQKLLD
                     KLTRYHSISTANSYPINHEAQVHGLFEQIAPAKIWQTNQHLTSYINRSAKSRTGVEAA
                     QWFKQQFDTLAQDYGRKDVESYFVKTGNKFIQPSVVTVIGKDKPGEAIVIGAHIDTLD
                     GNMPGADDDSSGISVELEMARVLFSSNFELNRPVYFIAYAAEERGLIGSGYVVQDFLQ
                     KNIPVKAVMQLDQAGYRANAKDQTIWLLKDYVDKGLTEFTAELLIRYVKIPVGYTKCG
                     YACSDHVNWANEGFKTTYPSATTLDDDNPYVHTSNDTLDILNLEHMVNFTKLGLAFIV
                     ELGLN"
     gene            2513..3403
                     /locus_tag="U6309_001516"
     CDS             2513..3403
                     /locus_tag="U6309_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504964.1"
                     /translation="MKTESLLSWLNVESNESYDSIRIKYQLRLEALEKEERIKKMGGI
                     NPFAPKIPLNNTRIITSKEIQEAWAAIDSLSKYQQFKGLVAQEENLSTIPNKKIDSKR
                     EETKLIHFVRERAQGPRGRRLPAHLQAKIKRVPLDKPHSKLITEGVEQQINAILDKTT
                     LSVSDLQSAVYTAVQNYTNYHMKSENKNNPKYNRGQGDGFFSFFRHGSFGITTANNLY
                     TSISAKNATLDETIEKLKDFLGHSSRAYHHHSFTSYLADALAEKGLLTSHPDSRYKQE
                     DVVNQLDQWIAQNSTVNLLK"
     gene            complement(3515..6445)
                     /locus_tag="U6309_001517"
     CDS             complement(3515..6445)
                     /locus_tag="U6309_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEN5504965.1"
                     /translation="MIDKMRKSKKPHSLNHIASGDNFNHLNLLERSLISQLIELSYDE
                     NTLHTLSLSGLKLLGKHPLLKDTITAVWHHDGSHINILASNGLKFDAPKELEKFLAVV
                     SDWHFLKIYKTKLKNYSEEKPKQRIDICLDKYQGNLFCISFWFDSIDCNFEILMPFFK
                     DILGILNLSLAKLMNKKREKLLTTILEKAGDSVEITNEEAVIQYVNPAFENITLYSAR
                     EAMNKTVASLLRSPVEDARLFEEINQTLKSGNTWKGQIRSRKKDGSYWVAQTIIVPVM
                     DDELGRITQHIAIKQDITEQVHRFNQLKISEERYRNLMNAVSDGIFIHNLQGAFLEVN
                     EAACLSLNYSREELINSYVWDIEVGFSQESLNQLWENVQKGPINTEGIHRRKDGTTFP
                     VDVRLAVFNATGEKLVLAIVRDITHQKRSEATIRKLTRALEQSPVLVIITDKTGTIEY
                     VNAKVIEQTGYRKEELVGGNTRILRSGQTPHEIYKTMWEHLANGEEWHGELLNKNKSG
                     EHFWVSAIISPIRNENEEITHYLAVMEDVTQKKSYEEMLKYQATFDNLTNLPNRLYAV
                     NKLERAISKSHINNQKLAVLFLDLDEFKQINDSLGHGVGDLLLKALSERFLSMIRQTD
                     TIARLGGDEFMMILENIMQDKDAESIAKKCREICSQPFVIESQELSVSTSIGIAIYPD
                     HGKDAKTLMRNADTAMYQSKIRGKNNWTVFQNTMIEGANNHIRIKTELHQAFTRNELY
                     ICYQPIIDLKKNKVVAAEALLRWQNTTLGQINPDQIIPLAEETGMIIPLGYWILDKVC
                     TQAKEWEFITGSKIKVAVNISTLQLKQKDFIHKVQLILKRTKLSADSLIFEVTESAFI
                     DDSKFILSQLNKLSDMGIHFSLDDFGVGYSFLQYIKSYPFKNLKIDRVFIQGVNTNHN
                     ELSLINSIITMARNLKLSVIAEGIETAEQLELMRSMNCDMVQGWYFSKPLSGDKFLFF
                     LMNK"
     gene            6656..7297
                     /gene="coq7"
                     /locus_tag="U6309_001518"
     CDS             6656..7297
                     /gene="coq7"
                     /locus_tag="U6309_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEN5504966.1"
                     /translation="MRTSSFLDRLIVEVDSALRTLVLPQKRITTRQSPAENLADTVLS
                     TQEKKHISGIMRVNHAGEVCAQALYQGQALTARLTHIKEQMANAAAEEVDHLAWCEER
                     LYELGSKPSLLNPIWYCGSVLLGALAGLAGDKISLGFVAETERQVTAHLQRHLHYLPE
                     KDKKTIAILKRMQEDEEHHAHTAMEAGAVELPYIIKQLMNAVSKLMTQSSYYI"
     gene            7314..8309
                     /locus_tag="U6309_001519"
     CDS             7314..8309
                     /locus_tag="U6309_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEN5504967.1"
                     /translation="MDYNTIFILFVIFIAFVFDFINGFHDAANSIATIVTTGVLTPRQ
                     AVLWAAFFNFIAFLIFNLTVAQTIGYGLIDTDLVDTYFILAALMGAIFWNLVTWYYGL
                     PSSSSHALIGGLAGAAIAKGGLSSLKIAGFAKVAAGIFISPVLGLLIGLLFTHIFSLF
                     LRHKNEEELNTVFKSFQLISSALLSITHGGNDAQKTMGIIAVLLFSASWLDGVFYVPF
                     WVVISCHGVISLGTLAGGWRIVHTMGTKITKLNTLKGCAAETGAAITIFAATEFGVPV
                     STTHTVTGSIAGVGLISGIGGAHWKVLRRIFYSWLLTIPAAALVAAAIMTISPTA"
     gene            complement(8319..9710)
                     /locus_tag="U6309_001520"
     CDS             complement(8319..9710)
                     /locus_tag="U6309_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN5504968.1"
                     /translation="MDLFRKKDIKGSLDNESHLAKCLSVFDLTFLGVGAIIGAGIFVL
                     TGIVAATISGPAVIFSYVVAGFACAFAALSYAELAASIGGCGSAYGYAYAGFGELIAW
                     IVGWDLLLEYSIAVSAVSIGWSGYANDFLMALKIFIPTHLLHGPADGGDLNILAILII
                     AVLTTLLIVGVKSSSRFNNIMVLVKLLVIFIFIAIAFGEVKVENWSSFMPYGWAGVMK
                     GASIIFFAYVGFDAVSTAAEEAINPQRDLPIGIIASLAICTVIYILVSGLLTGIAHYT
                     TLNVSSPISHVLLVLGYKTAAGLVGVGAIAGLTTVMLVLFYGLTRVFLAMSRDGLLPR
                     FLSKTNEHTKTPIRIILLCGILMASLASVTPIGTLAELVNIGTLFAFIVVCGGVLYLR
                     RTHPDMERPFKTPFMPYVPILGIISCSYLILNLPWVTILRFIIWMLVGIAVYFAFSRT
                     NSALSVEGDCAAE"
     gene            9870..10442
                     /gene="pagP"
                     /locus_tag="U6309_001521"
     CDS             9870..10442
                     /gene="pagP"
                     /locus_tag="U6309_001521"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HEN5504969.1"
                     /translation="MKRLISCLTIICALNASAAAETTSNPCSRWISFLKPVCQRIHQT
                     WTEGHDDMYFSGYAWHNRYVYSNEKIKSYNETAWGGGLGKSLFDEKGNWHGLYAIAFL
                     DSHRHLEPAVGYAYLKTASVNKDLKAGLGYSVLVTSRVDYDNVPIPGALPWAALFYKR
                     ITIAATYIPGSSREGHENGNVLYMLGKISL"
     gene            10582..11007
                     /locus_tag="U6309_001522"
     CDS             10582..11007
                     /locus_tag="U6309_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4949 domain-containing protein"
                     /protein_id="HEN5504970.1"
                     /translation="MMLKTQLTAFIGAVILAGSSLANPIKPEVCPSVPSIQSEGMSMS
                     SEILEGMYITYNLSHYNTSSSWVFIVGPIAAENDDMALAESNKLLSTMSGSPHPEDDG
                     EGNWICQYTTKSKDIIAFAIEADDMLSPLKMMRYLRTIR"
     gene            11154..11696
                     /locus_tag="U6309_001523"
     CDS             11154..11696
                     /locus_tag="U6309_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEN5504971.1"
                     /translation="MKSFIEQAQFYATYHQNQATRYTHMAGVPLIMLSIMIFLGFVKI
                     VIPGVYATDLACLATLATLIYYFLLNWQLALALTPILIFLLWLASWFNYDGPTKFGLW
                     VFIITFVVGWGLQFYGHFIEGKKPAFMVNFTQALIAPLYLTAELFFMAGLMNSLREQI
                     YGNEEVNTEEISKTKTKSKS"
     gene            complement(11766..13889)
                     /gene="rlmKL"
                     /locus_tag="U6309_001524"
     CDS             complement(11766..13889)
                     /gene="rlmKL"
                     /locus_tag="U6309_001524"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HEN5504972.1"
                     /translation="MNYSLFISCSKGLEYLLEDELKGLGLHVTQVSPQGVYGEASLPV
                     IYNLCLWSRLANRIQLILFSGHATKEQAVHQLCTDFHWQTVFTHDKTIAIEFHGASEQ
                     IRNTMFGAQIVKDGIVDHFRRLNGSRPSVDKEKPQILIHAHLKNDILTVSFDLVGYSL
                     HQRGYRKKAGKAPLKENVAAAMLLRAKWPELAAQGYSLHDPFCGSGTLVIEAAMMAAH
                     IAPGLLRQDQSLQYWARHQSSLWEKLRTQALQQVKPLAVKLIGTDADSKIITLARSNA
                     ERAGVLPLVEFNTLSLNACRPGTKRGLVVCNPPYGERLGEVTQLVPLYQELGTTLHTC
                     YQGWQAAILTSSPVLAKALGLRADKQYTLYNGPLECKLYCLTLSAANKLKNTPNAPLS
                     DNAQMLFNRLEKNRNHLQKWARKNQITCYRIYDADLPEYAYAIDIYNDYAVLQEYAPP
                     ASIPVHKAEKRSLEMLQVVPRALGIHPEKLIVKQRKQQKGSEQYQKIGKTSQRLIVTE
                     GKAKLIVNLYDYLDTGLFLDHRLMRLKFAQLEPGTRFLNCFCYTASASVHAALAGALT
                     TNVDLSKTYLLWAEDNFRLNDINLSKHQFLQYDCKEWMKTTRDKFDVIFLDPPSFSNS
                     KRMSDILDIQRDHVSLINMAMRLLNPDGVLYFSTNLRQFKLEPMLKEKYAVQDITPQT
                     IDQDFKRNSKIHHCFKIVMPHFADN"
     gene            complement(13886..14365)
                     /locus_tag="U6309_001525"
     CDS             complement(13886..14365)
                     /locus_tag="U6309_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEN5504973.1"
                     /translation="MDEQISKSQKKRDAHYLQDIGIKFIDLSASKLDSLPLPDNLRQA
                     IVDARSIKSHGAKRRQAQLIGKLMRAADFEAILAAYEKILEEESSVTASFHEIELWRD
                     RLLQDGKDALTEFIDAYQPEDVQQLRQLIKKALDDQMKEKNTGAAKALFRYLRSLIK"
     gene            complement(14557..15468)
                     /locus_tag="U6309_001526"
     CDS             complement(14557..15468)
                     /locus_tag="U6309_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEN5504974.1"
                     /translation="MAFFLITLCILILSFFCLAVMVLKFIQQPSLPLSFSQYVKLMCS
                     GVIAFIADTVGVGSFAVNVALAKLLGTFRDDELPAVNNGAQVLPGAIESLFFIYMVDV
                     DLTTLLTLVTGTCIGGLVGGFVVSQLSKQAIRLAMICSFALIIVLLIAHQLRLLPVGG
                     DLTELHSWKLVIGFFAMVVCGSLTSVGVGLFVMVQGVLFLMNVSPVVAFPIMTTAGAM
                     QQPLTTLVFLQKNKIPLKKTLILSFSGCLGVMITIPIFTKLTITWLHFLLLFILIYNF
                     LAIGHTYLRSRPVKQYVQSSVKLAAAD"
BASE COUNT         4903 a         3181 c         2795 g         4655 t
ORIGIN      
        1 tccataatga ccaaaacaaa cccataaagg ggctattttt ctactaaatg ccattttttg
       61 ccacaaataa atctggcttt taaaaaaaat atgttattct tcccatcatt actgattcgt
      121 gcttaatcaa agtggcgaat ttatttttta tcatttaact aaagacttta aatatgggat
      181 ggactgatcg attttggaat gcgtatcgcg tggttactaa taccgtaatc gaaggcgcag
      241 caaactcttt aaatgcagct ggatcactag cttgcctgct cggaggcgct ggagtctctc
      301 tctcttactc catgtctgaa acaataaaag caggatactt cggatcagtt ggagtagcag
      361 gcacagttaa tttggatgtg actgcagtag aatttaatta tggatttaat caaaccattc
      421 cttttcgcag agatttgctg gaaaaatccg gcggtggcag ctatgcattg aatgattttg
      481 ccaatccagg tactattttt atgattagtt ctctggctat tctttccgga accactttaa
      541 gaacttttgg ggctaatctt aaaaaatggc aacaaaacag agaagacaaa taccacttta
      601 ttgccaagta cagcactgac ctggaacccc cttcttacaa agaatatctg tatgtcaacg
      661 cagaatcttt cttcaattct ttggcaatag cctcattcag cagtcttttg gcaaaaaatg
      721 ttctggattt ttcgattaaa gatttatcga aatatcattt cacttatcct tttaatggaa
      781 caaatataac tggctcagtg aaaacgcctg attatactgg gccagttaca tcagaatttt
      841 tcccccttga cttttttgtg tctcaaaata caaccatcca cacagattat ttcaacctga
      901 tagtcaatga aaaagtgaag gccaatgcaa tgaccaatgt cacttatggt ggaggagtac
      961 atttagagcc cagccaccaa tcaagcgccc cccttctggc tgcctctgca actattggta
     1021 tcggtaccta tttggcaggc acttttttta gcaaaaaaat cctgcgagaa cgtgatgaaa
     1081 gacttcaacg ttcaagatca tcgagctatt ccatagtttc aggcactttt tgggataata
     1141 aacatgacat agaggcaaaa ggcgatacaa aattcgaaca tacccattca ttacagtaaa
     1201 gaatagccta agtctcctcc atgtaatata ttgcaatgaa aaaataaaac ggagtttttc
     1261 atggtattta aatcaaggtt agttggcatt attttattgt gcttttcaag tattaccgga
     1321 tggtgtcttt ctcagtccca tgaggaggat agaatcattg tttcaaactg cctgtatgag
     1381 caattaaagg acaaagccag gttaattgcg caatcggctc actttagctt cattgccatt
     1441 ccaattgatg aaaatattgc caatactcta caaaaaatga gaccagtttg tggaaattat
     1501 cttaatgctg agccatatat ccaacaaacc tacaatcgtt ttcttgattc gcaaaaatta
     1561 ttggataaac tgacccgcta ccattccatc tcgaccgcaa actcataccc gattaatcat
     1621 gaagcacaag tccatggttt gttcgaacaa attgctccag ccaaaatatg gcaaaccaat
     1681 cagcatttaa ccagttatat caaccgctct gccaaatcca gaacgggagt agaggctgca
     1741 caatggttta aacagcaatt tgatacctta gcccaagatt atggaagaaa agatgtagag
     1801 agttactttg tcaaaaccgg aaataaattt atacaaccat cagtcgtcac tgtaattgga
     1861 aaagataaac cgggagaggc cattgttata ggggcacata ttgacacgct ggacggcaat
     1921 atgcctggtg ccgatgatga ctctagtgga atatccgttg aacttgaaat ggcaagagtg
     1981 ctcttttcat cgaactttga gctcaataga ccagtttatt ttatagctta tgctgctgaa
     2041 gaaagaggac ttatcggctc tggatatgtg gtacaagatt ttttacagaa aaacattcct
     2101 gtgaaagcag taatgcagtt agaccaggca ggatacagag ctaacgccaa agaccaaacg
     2161 atttggctgt taaaggatta tgtagataag ggtttaactg aatttacagc agaattatta
     2221 attcgctatg tcaaaattcc tgttggctat actaagtgcg gttatgcatg cagtgatcat
     2281 gtgaattggg ccaatgaagg ctttaaaacc acctatccgt ctgcaactac tttagacgat
     2341 gataatcctt atgttcatac ctctaatgat actttggata ttctgaatct ggaacatatg
     2401 gttaatttca ctaaattagg cctggccttt atagtggagc ttggtttaaa ttagcgatga
     2461 atgcaataga gctcgtgcca atttaatggt gctctgcaat tggagtttat taatgaaaac
     2521 agaatcctta cttagctggc taaacgtaga aagcaatgaa tcctatgatt ctatcagaat
     2581 aaagtatcaa ctaagacttg aagccttaga gaaagaggaa cgcattaaaa aaatgggggg
     2641 tatcaatcct tttgcgccta aaatacccct taacaacacc aggataatca cctccaaaga
     2701 aatacaagag gcatgggctg ctattgactc tctcagcaag taccaacaat tcaaaggact
     2761 agtggctcaa gaagaaaatt tatccactat ccctaacaaa aaaatagata gcaagagaga
     2821 ggaaacaaaa ttaatccatt ttgtaagaga acgtgcacaa ggcccccggg gaagacgctt
     2881 gcccgcccat ttacaagcta aaattaaaag agtccctttg gacaaaccac acagtaagct
     2941 aattactgaa ggggtcgaac aacaaattaa cgcaatttta gacaaaacca ccttgtcagt
     3001 gagtgacttg caatctgctg tttacaccgc agtccagaat tatacgaatt atcacatgaa
     3061 atccgaaaat aaaaataatc ccaaatacaa cagaggacaa ggcgatggtt ttttcagttt
     3121 ttttcgccat ggaagctttg gaatcactac agcgaataac ctatacactt ctatcagcgc
     3181 aaaaaatgct actttagatg aaaccataga aaaattaaaa gattttttag gccattcctc
     3241 cagagcatat catcaccatt ctttcacatc ttacctggct gatgcacttg ctgagaaggg
     3301 actattaaca tcccatcctg acagccggta taaacaagaa gacgtagtga atcagcttga
     3361 ccaatggata gcacaaaact caacggttaa tcttttaaaa tgataatggc aaggatagtc
     3421 tgttttttac tcctgaagta aaaacaaata aattagaatc cgccaccgac atccgtgcca
     3481 aatctttaaa ttaggcacgg cagctggtac atttttactt attcattaaa aaaaacaaaa
     3541 atttgtcccc actcaaaggc ttggaaaaat accatccttg taccatatcg cagttcattg
     3601 agcgcattaa ctccagctgt tctgctgttt ctattccttc agcaataacc gataatttca
     3661 ggttacgcgc catagttata atactattaa tcaaacttaa ctcattatga tttgtattta
     3721 caccctggat aaaaacccga tcaattttta aatttttgaa cggataggac ttaatatact
     3781 gcaggaaaga gtaacccacc ccaaaatcat ccaaagaaaa atgaatgccc atgtcactta
     3841 atttgtttaa ttgagataaa atgaattttg aatcatcaat aaatgccgac tctgtaactt
     3901 caaaaattag agaatctgca gataattttg ttcttttcaa aatgagttgc accttatgaa
     3961 taaaatcctt ttgctttaat tgtaaggtag agatattcac cgctactttt atcttgctgc
     4021 cggtaataaa ttcccactct ttcgcttgtg tgcacacctt atccaaaatc caataaccca
     4081 atggaataat cataccggtc tcctcggcca agggaataat ctgatcggga ttaatttgac
     4141 ctaaggttgt attctgccaa cgtagcaagg cctccgcagc aacgacctta tttttcttca
     4201 gatcaatgat cggttggtag caaatatata actcattacg ggtaaacgcc tgatgtaatt
     4261 ccgtcttaat acggatatgg ttatttgccc cctcaatcat ggtattttga aataccgtcc
     4321 aattattttt gccccgtatc ttgctctgat acatggctgt atctgcatta cgcattaacg
     4381 ttttggcgtc cttgccatga tcaggatata ttgcaattcc tatacttgta gacaccgata
     4441 gctcttgtga ttcaataaca aaaggctgag aacaaatttc ccggcatttt tttgcgatac
     4501 tttcagcatc tttatcctgc atgatgtttt ctaaaatcat cataaattca tcaccaccta
     4561 agcgtgcaat agtatcggtt tgccgtatca tagatagaaa gcgctccgat aacgctttta
     4621 ataataaatc tccaacccca tgccctaagg aatcattaat ctgtttaaat tcatccaaat
     4681 ctaaaaacag caccgccaat ttttgattgt tgatgtggga ttttgaaata gctctttcca
     4741 atttattaac cgcatagaga cgattgggaa ggttggttaa attatcaaat gttgcctggt
     4801 attttaacat ttcctcgtag cttttcttct gagtcacatc ctccataaca gccagataat
     4861 gagttatctc ttcattctca ttacgaattg gagaaataat tgctgatacc caaaaatgct
     4921 ctccggattt gtttttattc agcaactcac catgccactc ttccccattg gctaagtgtt
     4981 cccacatggt tttataaatt tcatgaggag tttggcctga ccgtaaaatt cttgtatttc
     5041 caccaaccaa ttcttctttg cgataacctg tttgctctat cacttttgca ttgacatact
     5101 caatggttcc cgttttatca gttattatga ctaaaacagg gctttgctct aacgctcgtg
     5161 ttaattttct tatagttgct tcagagcgtt tttgatgtgt aatatcccgt acgatagcca
     5221 aaaccaattt ctccccagtc gcattaaaaa ctgccaaacg aacgtcaaca ggaaaagttg
     5281 tgccatcttt tctgcggtgt atgccctctg tattaatggg gcccttttga acattttccc
     5341 acaactgatt taaactttcc tgagaaaatc ccacttcaat atcccaaaca taggaattaa
     5401 ttaattcttc ccgactataa ttcaaagaca aacaagctgc ttcattaacc tctaaaaatg
     5461 ctccctgcaa attatggata aaaataccat cagatacagc attcatcaga tttcgatatc
     5521 gttcttcact gatttttagt tgattgaatc ggtgtacttg ttccgtaatg tcttgtttaa
     5581 ttgctatatg ttgggtaatt ctccccaatt catcatccat cacaggaacg ataatggttt
     5641 gagcgaccca atagctgcca tccttttttc ttgagcgtat ttgccccttc catgtattcc
     5701 ctgatttaag ggtttggttt atttcttcaa acaaccgagc gtcttcgacc ggtgagcgca
     5761 ataaggacgc cactgtttta ttcattgctt cacgggcact atacaaagtg atgttctcaa
     5821 aagcgggatt aacatattga atgactgcct cttcattcgt tatttcgacc gagtcgccag
     5881 ctttttccaa aatagtggtc aacagctttt cccttttttt attcatcaat ttggctaaag
     5941 acaggtttaa tatacccaga atatctttaa aaaaaggcat tagtatttca aagttacagt
     6001 caatagaatc aaaccaaaaa ctaatacaaa ataaattccc ttgatattta tcaaggcaaa
     6061 tatcgatacg ctgctttggt ttttcttcag aataattctt gagttttgtc ttatatattt
     6121 ttaaaaaatg ccaatccgag actaccgcta aaaatttctc caactccttt ggcgcatcaa
     6181 attttaatcc attagatgcc agaatattaa tatgactgcc atcatgatgc caaactgcgg
     6241 tgattgtatc ttttaatagg ggatgcttgc caagtaattt caacccactt aagcttaaag
     6301 tatgtagggt attttcatca taactcaatt caatcaattg gcttattaag cttcgttcta
     6361 acaggttaag atggttgaaa ttatctcctg aagcaatatg atttaatgaa tgtggctttt
     6421 tactcttcct cattttatct atcatgcgcc aatttagacc tacacctatc atttctgtat
     6481 aataaagctt aggatcttat tgtgtaataa tcaaaataga tgtataaaat ttactccatc
     6541 ttaactcgca gccaccgata ttaaatagcc ttataaaatt taattgacta atgacttcaa
     6601 actacacact aatcaagtta acatagagac cttacacttt atttttaaag tatccatgcg
     6661 aacatcaagc ttcctcgata ggttaatagt tgaagtcgat agtgcgcttc gaaccctcgt
     6721 tttgccacaa aaacgtatta caacgcgcca atctcccgct gaaaatctgg ctgatactgt
     6781 gctttccact caggaaaaaa aacatatttc agggataatg agagtaaatc atgccggaga
     6841 agtatgtgct caagcgcttt atcaaggaca agctttaaca gcacgactta ctcacatcaa
     6901 agagcaaatg gctaatgctg cagctgaaga agttgatcat ttggcctggt gcgaagaacg
     6961 tttgtatgaa ttaggcagca aacccagcct tttaaatccc atctggtatt gtggttcggt
     7021 tctattaggc gccctggctg gattggctgg cgataaaatc agcttgggat ttgttgctga
     7081 aacagagcgc caggtaaccg ctcatttaca aaggcatctg cattatctcc cggaaaagga
     7141 taaaaaaact atagccatat taaaaagaat gcaagaagac gaggaacatc atgcgcatac
     7201 agccatggaa gctggagcag ttgaacttcc ctatattatt aagcaattga tgaatgctgt
     7261 atcaaaatta atgactcaga gcagttatta tatttaacag gatatgatta catatggact
     7321 acaatacgat attcattcta tttgtcatat ttattgcgtt cgtatttgat tttatcaacg
     7381 gatttcatga tgccgccaat tcgattgcaa ccatagtcac tacaggtgtt ttgacgccaa
     7441 gacaagctgt actatgggct gcttttttta acttcattgc ttttctgatt ttcaatttaa
     7501 ccgtagccca aacaataggc tacggactaa tcgatacgga tctggtggat acttatttta
     7561 tcttagcagc cctgatggga gcaatattct ggaatttggt gacttggtat tatggactgc
     7621 catcaagctc ctctcatgcc ttaattggcg gattagctgg tgcagcaata gccaagggag
     7681 gcctgtcttc actcaagatt gcaggttttg ccaaagtagc ggcagggata tttatatctc
     7741 ccgttctagg attattgatt ggcttattat ttactcatat tttctctttg tttctcagac
     7801 ataagaacga agaggaactg aatacagtat ttaaaagttt tcaactgata tcttcagcat
     7861 tattgagtat cacgcatgga ggaaatgacg cacagaaaac gatgggtatc attgcggtat
     7921 tacttttttc tgcatcttgg cttgatggtg tattttatgt gccattttgg gtcgtcattt
     7981 catgtcatgg ggtaattagt ctgggaactt tggctggtgg ctggaggatt gtgcatacca
     8041 tgggcactaa aattaccaaa cttaacaccc tgaagggttg tgctgctgaa acaggagcag
     8101 ccattaccat tttcgctgca acagaatttg gagtacctgt ctcaaccacc catacggtca
     8161 ctggctccat tgcgggagtt gggttaatca gtggcatagg tggcgcccac tggaaagttt
     8221 tacgcaggat attctactct tggttgctta ccattccagc agcagcatta gttgctgccg
     8281 ctattatgac catctcacca actgcctaga ttacggcgtc actcggcagc acaatctccc
     8341 tcaacactta aggcactatt tgtcctgcta aatgcaaaat agaccgctat accaactagc
     8401 atccaaatta taaaacgcaa tatcgttacc catggcaaat tcaaaattaa ataagaacag
     8461 cttattatac ctaatatagg aacataaggc ataaaaggtg ttttgaatgg cctttccata
     8521 tcaggatgag ttcgccttaa atataaaaca cctccacaaa caacaataaa ggcaaacaag
     8581 gttccaatat taaccagctc ggccaaggta ccaattggag ttactgaagc gagagatgcc
     8641 attaatatgc cacatagcaa tataattcga attggtgttt tagtgtgttc gttcgttttt
     8701 gataagaacc ttggcaataa gccatccctt gacattgcta aaaatactct ggttaatcca
     8761 tagaacagaa ctaacataac tgtggttaaa ccagcaattg cgcctacacc gaccagtccg
     8821 gctgctgttt tataacccaa gacaagcagg acatgactga ttggtgatga aacgttaagg
     8881 gtagtgtaat gagcaatccc tgttaataaa cctgaaacca gaatatagat cactgtacaa
     8941 atagccagag aagcgattat ccctatgggt aagtctcgtt gaggattaat tgcttcttca
     9001 gcagcagtag aaacagcatc aaaccccacg taggcaaaaa agatgattga agcacctttc
     9061 ataacaccag cccaaccata gggcatgaaa gaagaccaat tttcaacctt cacttcacca
     9121 aatgcgatag ctatgaaaat aaaaataacc aaaagcttaa caagcaccat gatattatta
     9181 aatcgagaac tggatttcac accaacaatt aataatgtag ttaaaacagc tatgatcaaa
     9241 atggctaaaa tattgagatc cccaccgtca gcgggaccat gtaataaatg agttggaata
     9301 aatattttca gagccattaa aaaatcattg gcataaccac tccagccgat ggaaacagcc
     9361 gatacagcaa tagaatattc caataacaaa tcccatccga cgatccacgc gattaactca
     9421 ccaaaacctg cgtaagcata tccataagca ctgccacaac cccctatgct ggcagccagt
     9481 tcagcatagg ataaggcggc aaaggcgcag gcaaatccgg caacaacata ggagaatatc
     9541 acggctggtc cgcttatagt ggcagctact attcctgtaa gaacgaaaat accagcacct
     9601 ataatcgcgc ccactcccaa aaaggtaagg tcaaaaacac taaggcattt agctaaatgt
     9661 gattcattgt ctagcgaccc ttttatatct tttttacgaa acaagtccat gatagtatgc
     9721 tctcaggttt attttttatt gtttatttcc aaaatttaag ttatcaataa acataaactc
     9781 cggttatctt ccatctaaca aaaccttaca caattaaact ctaccattaa attatagcta
     9841 ggagaaaccc tgcaaaggtt aaaaattgaa tgaaaagatt aatttcctgc ttaaccatta
     9901 tttgcgcttt aaatgcgtcc gctgcagcgg aaacgacatc caatccttgt tctcgctgga
     9961 taagtttcct caagcccgtc tgtcaacgca tccaccaaac atggacagaa ggccatgatg
    10021 atatgtattt ttcaggctat gcctggcata accgctatgt ttacagtaat gaaaaaatta
    10081 agtcctacaa tgaaaccgca tggggtggtg gattaggtaa aagtcttttt gatgaaaagg
    10141 gaaactggca tgggctttac gcgattgcct tcctggattc ccatcgccat ttagagcccg
    10201 ccgttggtta cgcctatctc aaaacagctt cagttaataa agatttaaaa gcaggccttg
    10261 gatactcggt attagttacc tctcgggttg attatgataa tgtccccatc cccggagcat
    10321 taccgtgggc tgctcttttt tataaaagaa taacgatcgc agcaacctat atacctgggt
    10381 ccagtcgtga aggacatgag aacgggaatg tattgtatat gctcggaaaa atatccttat
    10441 aaagatggat taacttttgc tagctgcaaa aggtcatgtg aaacaaaccc tgattagatc
    10501 cctttaaaac ggttgcaact gaaaattcaa cttgttagtc ttttgaagat ttttaactgc
    10561 taagtaacaa ggagaagtaa catgatgttg aaaacccagt tgactgcttt tatcggtgct
    10621 gtaatcttgg ctggctcttc tttagcaaat ccaataaaac ctgaggtatg ccccagtgta
    10681 ccctctattc aatcggaagg aatgtccatg tcttctgaaa ttttagaggg catgtacatc
    10741 acctataatt taagtcatta caataccagt tcaagctggg tgtttattgt agggccaatc
    10801 gcagctgaaa acgatgatat ggcattggca gaaagcaata aattactttc aaccatgtca
    10861 gggtctcccc atccggaaga tgatggagaa ggcaattgga tatgtcagta tacaaccaaa
    10921 tccaaagaca ttattgcatt tgccatagag gcagatgata tgctttctcc attgaaaatg
    10981 atgagatatc tcagaacaat ccgctgataa gtggataaca cctgcacgga cacggaagca
    11041 tgatttgctt ccgtgattta acgaatagtt aagaactcct cctctctttt tggtacttct
    11101 ttattttatg tgctatgttt tcttcatgac cctaacttca ggatgaaaag aaaatgaaat
    11161 cgtttattga gcaagctcaa ttctatgcaa cctaccacca aaaccaggcg acacgttata
    11221 cacatatggc aggagtcccg ttaatcatgc tttctatcat gatttttctg ggatttgtta
    11281 aaatagttat tccaggtgtt tatgcaactg atcttgcttg tttggctacg cttgccactc
    11341 tcatttatta cttcctatta aactggcaac ttgccttggc attaactccc attttgatct
    11401 ttttgttatg gctggccagt tggtttaact atgatggccc aacgaaattc ggactttggg
    11461 tatttattat tacttttgtt gtaggctggg gattgcaatt ttacggtcat tttattgaag
    11521 gaaaaaaacc tgcttttatg gttaatttta cccaagccct tattgcgcct ttgtatttaa
    11581 cagcggaatt gttctttatg gcaggactaa tgaattccct gagagaacaa atttacggga
    11641 acgaagaagt aaatactgaa gaaataagca aaaccaaaac aaaatccaaa tcttaaagag
    11701 agtcgagctt cacgcttatg aaagggaagc tcggcaagag atctaataat aaataactga
    11761 attattcagt tatccgcaaa atgcggcatt acaattttaa aacaatggtg aattttgcta
    11821 tttcgtttaa aatcctgatc gatggtctgc ggagtaatgt cttgtactgc atatttctct
    11881 tttaacattg gctctaactt aaattggcgc agattagttg agaaatataa aacaccatca
    11941 ggatttaaca accgcattgc catgttaatc aaagagacat gatctctttg aatgtctaaa
    12001 atatcgctca tacgctttga gtttgaaaag ctggggggat ctaaaaaaat aacatcaaat
    12061 ttatccctgg tagttttcat ccattcctta caatcgtatt gcaaaaattg atgcttggat
    12121 aaattaatat catttaatct aaaattatct tccgcccaaa gcaaatacgt tttggataaa
    12181 tcaacattcg tggttaatgc accggccaag gcagcatgga cgcttgcact ggcggtataa
    12241 caaaaacagt tcaaaaatcg agttcccggc tctaattgag caaatttcaa gcgcataagt
    12301 ctatgatcca gaaatagtcc tgtgtcaaga taatcataca aattcactat caattttgcc
    12361 ttaccttctg tcactatcaa tctttggctg gttttgccta ttttttgata ttgttctgag
    12421 cccttttgct gctttctttg tttaactatt aatttttcag ggtgaatacc taaggcccgt
    12481 ggaacgactt gcagcatctc aaggctgcgt ttttctgcct tgtgtactgg aatgctggct
    12541 ggtggcgcat attcttgcag tacagcatag tcattatata tgtctatggc ataggcatat
    12601 tcgggtaaat ccgcatcgta aattcgataa caggtgattt gattttttct tgcccatttc
    12661 tgtaaatgat tcctgttttt ttccaatcgg ttaaatagca tttgtgcatt atcagaaagc
    12721 ggggcattcg gggtattttt tagtttattt gccgcagaaa gagtcaaaca atagagtttg
    12781 cactctaatg ggccattata taaagtatat tgcttatcag ctcgtaatcc taatgcttta
    12841 gccaaaactg gactggaagt taaaatagcc gcttgccaac cttgataaca agtatgtaat
    12901 gtagtaccca attcttgata taaaggaaca agttgagtta cctcccccaa tcgctcaccg
    12961 taaggtggat tacaaacaac taatcctctt tttgtccctg gtctacaagc atttagcgat
    13021 aatgtattaa attcaaccaa aggtaatacg cctgctctct cagcattgga acgcgcaaga
    13081 gtaattattt tgctgtcagc gtcagtacca atcagcttaa ctgccagcgg cttcacttgc
    13141 tgtaatgcct gagttcgcaa tttttcccac aaagaactct gatgccgagc ccaatattgt
    13201 aaggattgat cttgtcgtaa taaacctgga gcaatatgag ctgccatcat ggctgcttct
    13261 ataactaatg ttccagagcc acaaaacgga tcatgtaagc tatatccctg tgcggctagc
    13321 tctggccatt tggcccttaa taacatggct gctgcaacgt tctcctttaa aggagccttg
    13381 cctgcttttt tacgatatcc tctttgatgc aggctatatc caaccaaatc aaaactcacc
    13441 gttaaaatat catttttcaa atgagcgtga attaaaattt gcggtttttc tttgtcgaca
    13501 gaaggccttg aaccattcaa gcgacgaaaa tgatccacta ttccatcttt gactatctga
    13561 gcgccaaaca tggtattacg aatttgctca gaggcaccat gaaattcaat agctatggtt
    13621 ttatcatggg taaaaacagt ttgccaatga aaatcggtac ataactgatg tactgcttgc
    13681 tcttttgtgg catggccact aaataaaata agctgtattc tgttagcaag tcttgaccat
    13741 aaacataaat tataaattac aggcaggcta gcctcaccat agacaccttg aggactcacc
    13801 tgagtcacat gaagccccaa tcccttgagt tcgtcttcca gcaaatactc caaacctttt
    13861 gaacaactaa tgaatagaga gtaattcatt ttattaaaga ccttaaatag cgaaacagtg
    13921 ctttagcagc acccgtattt ttctctttca tctgatcatc taatgccttt ttaatcagtt
    13981 gccttaattg ttgcacgtcc tcaggttgat aagcatcgat aaactctgtc aaagcgtctt
    14041 ttccatcttg taataacctg tcgcgccaca gctctatctc atgaaaagag gccgtaactg
    14101 aactctcttc ttcaaggatt ttctcatagg cagcaagaat ggcttcaaaa tcggctgcac
    14161 gcattaactt gcctattaat tgcgcctgtc gtcttttagc cccatggctt ttgattgacc
    14221 tggcgtcaac aattgcttga cgtaaattat cgggaagggg caaagaatcc agtttggatg
    14281 cgcttaaatc aataaattta atgcctatat cttgtaaata atgagcatct ctttttttct
    14341 gtgatttact gatttgttca tccatagggc gatatattta ttaaaataac gttaattata
    14401 ctatataagt acctatccag ataggatagg ctctaatcaa ttttgaataa aaacaagcaa
    14461 ataccaacat caacctgcat catcctatta gttagccata gctttaaaag ccctgttccc
    14521 gaaaaaacaa tgaagaaaaa ttataaactg agagactcag tccgctgccg caagtttaac
    14581 agatgattgg acgtattgtt tgacaggtct tgatcgtaaa taagtatgac caattgctaa
    14641 aaaattatag ataaggataa ataaaagtaa aaaatgcagc caggttattg ttaattttgt
    14701 aaaaattgga atggtgatca taacacccag acatcctgaa aagcttaaaa tcaaagtctt
    14761 ttttaacggg attttatttt tttgtaaaaa aactaatgtg gtcagcggct gttgcatggc
    14821 tcctgcagtt gtcatgatag gaaaagcgac aactggtgaa acattcatta aaaataaaac
    14881 accttgcacc atgacaaaaa gccctactcc gacagaggta agagagccac aaactaccat
    14941 agcaaaaaag ccaataacca gcttccagga atgcaattct gtcaaatcac cacctactgg
    15001 caatagacgc aattgatgag ctattaaaag aacaatgatt agggcaaatg aacaaatcat
    15061 tgccagtcta atggcttgct tgcttaactg gctaactaca aaaccaccta ctaacccacc
    15121 aatacaggtt ccggtaacta aagtaagtag cgtagttaaa tcaacatcta ccatgtatat
    15181 gaaaaataaa gattcgatag cgccagggag gacttgagcg ccattattta cagcaggtaa
    15241 ttcatcatca cgaaaagtgc ctaataattt tgccaaggct acattaacgg cgaaactgcc
    15301 gacccccact gtatctgcaa taaaagcaat aactccgcta cacattaatt taacatattg
    15361 ggagaaggat agaggcaagg aaggctgctg aataaatttt aaaaccatta cggccaggca
    15421 gaaaaaactc aatattagta tgcaaagcgt gattaaaaaa aatgccattc tgttcctaat
    15481 acgaaagtaa tatataatcc caatgatttt aaaatcaaag cttattaagg tttc
//
LOCUS       DASLOE010000062        15438 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.062, whole genome shotgun sequence.
ACCESSION   DASLOE010000062 DASLOE010000000
VERSION     DASLOE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15438
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.062"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            59..134
                     /locus_tag="U6309_001527"
     tRNA            59..134
                     /locus_tag="U6309_001527"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:92..94,aa:Trp,seq:cca)
     gene            148..519
                     /gene="secE"
                     /locus_tag="U6309_001528"
     CDS             148..519
                     /gene="secE"
                     /locus_tag="U6309_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEN5504975.1"
                     /translation="MKSSNENQSKMKDIMSWLAIILATAGAFVCTYYYTFTGPIQSII
                     WLAWLILILFLGYLTTTGKRVFAFAQEAKVELLKVVWPTRQETIQTTTIVMVMVGLTG
                     FILWGVDSIMMWAIAKLTHLG"
     gene            522..1070
                     /gene="nusG"
                     /locus_tag="U6309_001529"
     CDS             522..1070
                     /gene="nusG"
                     /locus_tag="U6309_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEN5504976.1"
                     /translation="MVEENKAKQWYVVHAYSGYENFVMREITSRAKHHNLQDKIGEVV
                     VPSEEVVEMRSGQKRKSTRKFFPGYVLVHMVMDDQTWHMVRGIPRVLGFIGGTSQTPT
                     PISDKEAQAIMQRVEDGVTKPRPKILFEPGEVIRVKEGPFVDFNGVVEEVNYEKNRLR
                     VAVLIFGRSTPVELEFSQVEKT"
     gene            1206..1640
                     /gene="rplK"
                     /locus_tag="U6309_001530"
     CDS             1206..1640
                     /gene="rplK"
                     /locus_tag="U6309_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEN5504977.1"
                     /translation="MAKKVEAYIKLQIPAGKANPSPPVGPALGQRGVNIMEFCKAFNA
                     ATQQMEQGLPIPVVITVYSDRSFTFITKTPPASVLLKKAAGIQSGSGTPNTKKVAKLN
                     VSQLEEIAKVKKPDLTAADLAAAVRSIAGTARSMGIEVEGLE"
     gene            1650..2345
                     /gene="rplA"
                     /locus_tag="U6309_001531"
     CDS             1650..2345
                     /gene="rplA"
                     /locus_tag="U6309_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEN5504978.1"
                     /translation="MSKLTKKQKKIAEVIKPNQLYTVADAVAILKQFASKKFRESLDI
                     SINLGVDPRKSDQVVRSSTNLPKGTGKTVRVAVFAQGDNAAKATAAGADIVGFEDLAD
                     KIKAGEMNFDVVIATPDAMRIVGQLGQILGPRGLMPNPKVGTVTTNVEAAVNDAKSGQ
                     VRYRTDKNGIIHCTVGKADFAPEDVLENVVALINDLKKAKPASSKGQYLKKISLSTTM
                     GPGLPIDISSIPV"
     gene            2518..3051
                     /gene="rplJ"
                     /locus_tag="U6309_001532"
     CDS             2518..3051
                     /gene="rplJ"
                     /locus_tag="U6309_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEN5504979.1"
                     /translation="MTLNLAAKKAVVEEVTAVASKAISAVVADYRGLTVNQMTQLRSE
                     ARKSGVYLRVVRNTLTRRAFKNTEFECLNDLLVGPVFIALSLEAPSDAARLLKDYAKT
                     FEKLEIRALSVGGKVYNANQIDAVASLPTRDEAISKLMYVMKAPIEKFVRTLAEPHAK
                     LARTLAAVKDKKAGNPA"
     gene            3082..3462
                     /gene="rplL"
                     /locus_tag="U6309_001533"
     CDS             3082..3462
                     /gene="rplL"
                     /locus_tag="U6309_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEN5504980.1"
                     /translation="MAVSKNEILETISNMTVMEVVELIEAMEEKFNVSAAAAAVAVAA
                     PAAGAGAAAAEEQTEFTVVMTSFGSNKVNVIKAIRGITGLGLKEAKDLVEGAPSTVKE
                     GVSKDEAASIKKELEEAGATVEVK"
     gene            3554..7660
                     /gene="rpoB"
                     /locus_tag="U6309_001534"
     CDS             3554..7660
                     /gene="rpoB"
                     /locus_tag="U6309_001534"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN5504981.1"
                     /translation="MAVAEAKPQYSHAEKKRFRKSFGKQTDIMPIPNLLEIQLKSYRD
                     FLQTDTKLSEQLNTGLHAAFSSVFPIESFSGNARLEYVGYKLGEPAFDVRECKLRGLT
                     YSAPLRVKIRLVVLDKDASDDPKPIKDIREQDVFMGEIPLMTDVGTFVVNGTERVVVS
                     QLHRSPGVIFEHDKGKTHSSGKLLYSARIIPYRGSWLDFEFDPKDCVYVRIDRRRKLP
                     VTILLRALGYEAEDILSEFFETTRCHLKNGEYHIDLIPQRLRGEIASFDIHVPETGEL
                     IVEQGRRITARHIKQMEKSQMQDLVVPRDYLIGKTLAKNIIDTSTGEFLAQANDEITE
                     ELLDAMANHGILQIDMIYTNDLDHGSYISDTLKIDPTGSQLEALVEIYRMMRPGEPPT
                     KEAAEALFKNLFFVEERYDLSAVGRMKFNRRVGIKSDEGPGTLTKEDILSVIKTLIDI
                     RNGIGMVDDIDHLGNRRVRSVGEMTENQFRVGLVRVERAVKERLSLVESENLMPQDLI
                     NAKPVSAAIKEFFGSSQLSQFMDQVNPLSGVTHKRRVSALGPGGLTRERAGFEVRDVH
                     TTHYGRVCPIETPEGPNIGLINSLSVYARTNEYGFIETPCRKVVNGRVTDEVEYLSAI
                     EEVDQYIAQSNVELDEQGNILADLVPCRHQNEFSLTTPDKINYMDVSPKQIVSVAASL
                     IPFLEHDDANRALMGSNMQRQAVPTLRSEKPLVGTGMERIVASDSGVSVVAKRGGVID
                     LVDASRIVVRVNDDETTAGETGVDIYNLTKYFRSNQDTCINQRPIVSTGDRIQRGDVL
                     ADGPCTDMGELALGQNLLVAFMPWNGYNFEDSILISERIVHDDRFTTIHIEELTCIAR
                     DTKLGTEEITADIPNVGESALSNLDESGVVYIGAEVKAGDILVGKVTPKGETQLTPEE
                     KLLRAIFGEKASDVKDSSLRVPSGMNGTVIDVQVFTRDGLEKDARAKSIEEEHLARVR
                     KDLIDERRIREEDIYHRVSHLLLDRVATGGPGSLKPGSKITQDYLDKVEREKWFDIRI
                     EDDAISQQLEQLSKQLELLTKEMEKRFNDSRKKIIQGDDLAPGVLKIVKVYLAVKRRI
                     QPGDKMAGRHGNKGVISIVVPVEDMPHMEDGTAVDIVLNPLGVPSRMNIGQVLETHLG
                     LAAKGLGRKIAQMLDEKQTPEAIKAYLEKIYNHDGVQRVNLKCLNDDELMTLADNLRA
                     GVPMATPVFDGATEQEIKSMLQLADLPADGKTVLIDGRTGNKFDNPVTVGYMYMLKLN
                     HLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVK
                     SDDVGGRTKIYKNIVDGDHRMDPGMPESFNVLLKEIRALGIDIELEHD"
     gene            7749..11954
                     /gene="rpoC"
                     /locus_tag="U6309_001535"
     CDS             7749..11954
                     /gene="rpoC"
                     /locus_tag="U6309_001535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032829172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEN5504982.1"
                     /translation="MSDLLGILKQQGQSEEFDAIKIALASPELIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKTFGPVKDYECLCGKYKRLKHRGVICEKCGVELALAKVRRERM
                     GHIELASPVAHIWFLKSLPSRIGLLLDMTLRDIERVLYFEAFVVVDPGMTELERGQLL
                     NDEAYLDAMEQYGDEFDARMGAEAIRDLLRQIDLEDEIRNLREELPTTNSETKIKKIT
                     KRLKLLEAFYESGNKPEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLNAPDIIVRNEKRMLQESVDALLDNGRRGRAITGTNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPTLKLHQCGLPKKMALELFKPFIFSKLEFRGLAT
                     TIKAAKKMVEREESVVWDILDDVIREHPILLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCTAYNADFDGDQMAVHVPLTLEAQLEARSLMMSTNNILSPASGEPIIVPSQDVVL
                     GLYYLTREKVNALGEGKIYSSAQEAQNFYEAGHLDIHAKIKIRMPKEDGETGYHLVET
                     TVGRAILAEILPKGMPFDYINRTMTKKVISKVIDSCYRKFGLKETVIFADQLMYTGFK
                     YATRSGASIGIEDMEIPDDKASIIEHADNEVREIESQFRSGLVTNGERYNKVIDIWSR
                     TNELVAKSMMSKIATEEVTDAKGNKVRQESFNPIFMMADSGARGSAAQIRQLAGMRGL
                     MAAPDGSIIETPITANFREGLNVFQYFISTHGARKGLADTALKTANSGYLTRRLVDVA
                     QDVVITEDDCGTDTGILMQPLIEGGDIVEPLHERVLGRVVASDVYIPTQTEPVVKAGT
                     LLDEEWVEKLEKHGVDQVMVRSPITCQTRFGLCAKCYGRDLARGHLVNTGEAVGIIAA
                     QSIGEPGTQLTMRTFHIGGAASRATAANNIQIKTKGVIRLHNIKTVTHENKNLVAVSR
                     SGEVTIVDEFGRERERYKVPYGAVISAQDNSPVEAGQVIATWDPHTHPVISEVSGRLK
                     FVDLIDGITMNRQTDELTGLSNIVIIDAKQRSAAGRDLRPMVKLVTDEGDDIYLAGTN
                     VPAQYYLPVDAIVNFEDGSLVGIGDVIARIPQERSKTRDITGGLPRVADLFEARKPKD
                     SAVMAEVSGLVNFGKETKGKRRLIINVSEDQCHEELIPKWRHISVFEGEHVERGEIIA
                     EGALNPHDILRLLGVGALANYIVNEVQDVYRLQGVKINDKHIEVIVRQMLRKRVITFA
                     GDSKFLVGEQVEESAMLQENDKLLAEGKQIARGTPILLGITKASLATESFISAASFQE
                     TTRVLTEAAVSGKVDELRGLKENVMVGRLIPAGTGYTYHQSRKAKRARAAAGGDSSAT
                     HTVTASDVEHALSEALNADNHEH"
     gene            12068..12448
                     /gene="rpsL"
                     /locus_tag="U6309_001536"
     CDS             12068..12448
                     /gene="rpsL"
                     /locus_tag="U6309_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEN5504983.1"
                     /translation="MATINQLVRKPRVDVKKKSNVPALESCPQRRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTSGVNDRKQGRSKYGTKKPKDKK"
     gene            12469..12996
                     /gene="rpsG"
                     /locus_tag="U6309_001537"
     CDS             12469..12996
                     /gene="rpsG"
                     /locus_tag="U6309_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEN5504984.1"
                     /translation="MPRRREVPKREILPDPKHHSELLAKFINVLMVSGKKSIAEKITY
                     GALSVMEERVKKIKKNEEDGSETGSSGSAGAVLRYFEEALDNVRPSVEVRSRRVGGAT
                     YQVPVEVRHDRSIALGMRWIVQAARTRGEKGMMLRLAGELMDAYENKGSAVKKREDTH
                     KMAKANQAFAHFRWN"
     gene            13011..15095
                     /gene="fusA"
                     /locus_tag="U6309_001538"
     CDS             13011..15095
                     /gene="fusA"
                     /locus_tag="U6309_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN5504985.1"
                     /translation="MATPLKLYRNIGIAAHVDAGKTTTTERVLYYTGMSHKIGEVHDG
                     AATMDWMVQEQERGITITSAATTCYWSGMDKQFESHRINIIDTPGHVDFMIEVERSLR
                     VLDGAVVVFDSVAGVEPQSETVWRQANKYGVPRIVFVNKMDRMGANFLRVVSQIKQRL
                     GSTPVVLQLPIGAEEEFKGVIDLIKMKAIHWDEENKGMTFKYVDIPADLKATCEEYRA
                     HIIEAAAEYSEELMEKYLEGEEFTEAEIKKALRHLTITNKVVPVFCGSAFKNKGVQAV
                     LDGVIEYLPSPTDIPDIQGVDEHGDEIHRKTSYDEPFSALAFKIATDPFVGTLTYFRA
                     YSGILKSGDTVYNSVKGKKERIGRLLQMHANSREEIKEVRAGDIAAAVGLKTVTTGDT
                     LCDQDKVVILERMDFPDPVIAVAVEPKTKADQEKMGIALGKLAQEDPSFRVHTDEESG
                     QTIIQGMGELHLEIIVDRMKREFNVEANVGKPQVAYRETLKQAVEQEGKFVRQSGGRG
                     QYGHVWLKIEPQEPGKGYEFINAIVGGVIPKEYIPAVDKGIQEQMQNGVIAGYPVVDV
                     KVTLFDGSFHEVDSSEMAFKIAGSQCFKQGALKAKPVLLEPIMSVEVVTPEDYMGDVM
                     GDLNRRRGLVQGMEDSPAGKIVRAEVPLAEMFGYSTDLRSATQGRATYTMEFCKYAEA
                     PTNIAEAIIKKQ"
     gene            15116..>15438
                     /gene="tuf"
                     /locus_tag="U6309_001539"
     CDS             15116..>15438
                     /gene="tuf"
                     /locus_tag="U6309_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN5504986.1"
                     /translation="MAKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTA
                     KAYDQIDAAPEERERGITISTAHVEYESASRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSA"
BASE COUNT         4989 a         2712 c         3610 g         4127 t
ORIGIN      
        1 aggaaaaagt acaaaagtgg catgagcctt atatacatgg ctcatgtcaa taaaatttag
       61 gccagtagct caattggcag agtggcggtc tccaaaaccg caggttgggg gttcgattcc
      121 ctcctggcct gccaactaca agtaaatatg aaaagttcaa acgaaaatca aagtaagatg
      181 aaagatataa tgtcctggct agctatcata ttagctacag cgggagcttt tgtatgtact
      241 tactattata cgttcacagg accaattcaa tcgataatat ggcttgcgtg gcttattttg
      301 atccttttct tgggttactt aacaacaaca ggtaagagag tctttgcgtt tgcgcaagaa
      361 gcaaaagttg aattattaaa agtggtttgg cctacacggc aagaaacaat acaaacaact
      421 actatagtaa tggtgatggt tggattgaca ggatttatac tctggggagt agattcaatc
      481 atgatgtggg caattgctaa gttaacacat ttagggtgaa tatggtcgag gaaaacaaag
      541 caaaacagtg gtacgttgta catgcctatt caggatatga aaattttgtc atgcgcgaaa
      601 taacatctcg tgcaaaacac cacaatttac aagataaaat aggcgaggta gttgtacctt
      661 cagaagaagt tgttgaaatg cgctcaggcc aaaagcgtaa aagtacccgc aagtttttcc
      721 caggatatgt cctcgttcat atggtaatgg atgatcaaac atggcacatg gtcagaggaa
      781 tacccagagt tctgggcttt attggcggaa ccagccaaac cccgactccg atttctgata
      841 aagaagctca ggccattatg caacgagttg aagatggcgt aactaagcca aggccaaaaa
      901 tactgtttga gcctggagaa gtaatcagag tgaaagaagg tccatttgtt gactttaatg
      961 gagttgttga agaggtcaat tatgagaaaa acaggctaag agtcgcagtt cttatttttg
     1021 gacgttcaac tccagtagaa cttgagttca gtcaagtaga aaaaacataa atgatttaga
     1081 taagaattta aatttattgt actgttaaaa atttaaattt aaaggtttta caaaaccggg
     1141 gagctaacac ttaattgaaa atgctaattc aataaaagct agcgcatgac ccataaggag
     1201 taataatggc aaaaaaagta gaagcatata ttaaattaca gatccctgca ggaaaagcaa
     1261 atcctagccc tccagttgga cctgctttag gtcaacgtgg tgtaaatatt atggaattct
     1321 gtaaagcatt caatgctgcg acacagcaaa tggaacaagg tcttcctatt ccagttgtta
     1381 tcactgtata tagtgatcgt agttttacct ttataaccaa gacccctcca gcttcagttc
     1441 tgctgaaaaa ggctgctggc attcaaagcg gaagtggcac accaaatact aaaaaagtag
     1501 ccaaactgaa tgttagtcaa cttgaagaaa ttgctaaggt caagaaacct gatctgacag
     1561 cggctgatct agcagcggct gtaagaagta ttgcaggtac agcacgtagc atgggtattg
     1621 aagttgaagg tttggaataa ggggttacca tgtcaaaatt aactaagaaa caaaagaaaa
     1681 tagcggaagt tattaagcca aatcaactct atacagttgc tgatgctgta gctattctaa
     1741 agcaatttgc atcaaaaaaa tttcgtgaga gccttgatat cagtattaat ttaggagttg
     1801 atcctcgtaa gtcggatcaa gtagtacgat cttccaccaa tctgccaaaa ggtacaggaa
     1861 aaacagtgcg agttgccgtt tttgcccaag gtgataatgc tgctaaagca actgctgcag
     1921 gtgccgatat tgtaggattt gaagatctgg ctgataaaat caaagcaggc gaaatgaatt
     1981 ttgatgttgt tattgcaact ccagatgcta tgagaatcgt aggtcagctc ggacaaattt
     2041 tgggaccgag gggactcatg cccaatccta aggttggaac agtgacaact aacgtggaag
     2101 cagcggttaa tgatgctaaa tcaggacaag taagatacag aactgataaa aatggaatta
     2161 ttcattgtac tgttggaaag gcagattttg ctcctgaaga tgttcttgaa aacgtagttg
     2221 cgctaatcaa tgatctgaag aaggcaaagc cagcatcctc taaagggcaa tatctgaaga
     2281 aaatatcttt atctacaaca atggggcctg ggttgcctat tgatatttca tcaatacctg
     2341 tgtaacatac catcccattt ttaagaattc acggcataga ttaggttcca tgccgtcgaa
     2401 gaccgcaggt gcctaggctt aatttcctgc gcagacggtg aaccggctcc ttaataaaga
     2461 gacggccacc ataactgtca agtccatgag atttgtacat attaggaggt cagtaacgtg
     2521 acattaaatt tagctgcaaa aaaagccgtt gttgaagaag tgactgcagt cgcgtcaaag
     2581 gctatttcag cagtggttgc tgattatcgt gggttaactg taaatcaaat gacgcaattg
     2641 cgtagtgaag cacggaaatc cggtgtttat cttcgtgtgg tgcgaaatac actaacaaga
     2701 agagcgttta aaaacactga atttgaatgc ttgaatgacc tacttgtagg tccagtattc
     2761 attgcattgt cgctggaagc accaagtgat gctgcaagac tactgaaaga ttacgcaaaa
     2821 acatttgaaa aacttgagat tagagcatta tcagttggcg ggaaggtata caacgcaaat
     2881 caaatagatg ctgttgcgag cttgccaaca cgtgatgaag caatatccaa gttaatgtat
     2941 gtgatgaaag cgccaattga gaaatttgtt cgtacacttg cagagccaca tgccaagttg
     3001 gctagaactc ttgctgctgt aaaggacaaa aaagcaggca atcccgctta atcattattt
     3061 aattttaatt aggagctagt aatggctgta tcaaaaaacg aaattttaga aacaatttct
     3121 aacatgacag taatggaagt tgttgaatta atcgaggcaa tggaagaaaa atttaatgtt
     3181 tctgctgccg ctgctgccgt agctgttgct gcacctgctg caggtgctgg tgccgctgct
     3241 gctgaagaac aaacagagtt tactgttgta atgaccagct ttggttctaa caaggtaaac
     3301 gtaatcaagg caattcgtgg cataactggt ctgggcttga aagaagctaa agacttagta
     3361 gaaggtgcac catcaactgt taaggaaggt gtatcaaaag atgaagctgc cagtatcaag
     3421 aaagagcttg aagaagctgg tgctacagta gaagttaaat aattaaaata tagcagtatt
     3481 taagaaccca gccatgttca catggctggg tttactcgtt tgaaaatcat caataattta
     3541 cagaggaacc accatggcag ttgcagaagc taaacctcaa tattcgcatg ctgagaaaaa
     3601 acgatttcgc aaaagctttg gtaagcagac agatataatg ccaataccga atctgcttga
     3661 aattcaactt aaatcctaca gggattttct tcaaactgat actaaattaa gcgaacaact
     3721 gaatacggga ttgcatgctg cattttcttc tgtatttcct atcgaaagct tttctggtaa
     3781 tgcaagactt gaatacgtcg gatacaagct tggagagcct gcatttgatg ttcgcgaatg
     3841 caaattaaga gggctaactt actcagctcc tctaagagtc aagatcagac tcgttgtgct
     3901 tgataaagat gcaagcgatg atcccaagcc tataaaggat attagagagc aagatgtttt
     3961 tatgggggaa attcccctaa tgaccgacgt tggtacgttt gtggtcaatg gtactgagcg
     4021 tgttgttgtt tcacaattac atcgttcgcc tggagttata tttgaacatg acaagggaaa
     4081 aactcactct tcaggtaaat tgctctattc cgcacgaata ataccttatc gtggctcatg
     4141 gttagatttt gaattcgatc ctaaagattg cgtttatgta cgtattgaca gacgtcgaaa
     4201 actgccagtg accattttgc tcagagcact gggctacgaa gcagaagata tattaagcga
     4261 attttttgaa acaactcgtt gtcatctaaa aaatggcgaa taccatatcg atttaattcc
     4321 acagcgtttg cgtggtgaaa tcgcctcatt cgacattcat gttcctgaga caggagagct
     4381 tatcgtagaa caaggtcgtc gaattactgc aagacatata aagcaaatgg aaaaaagcca
     4441 aatgcaggat cttgtggtgc caagagacta tttaattgga aaaacattag caaaaaatat
     4501 tattgatacc tcaaccggtg aatttctggc tcaagcgaat gatgagataa cggaagagct
     4561 tttggatgcc atggctaacc atggtatttt acaaattgat atgatctata ctaatgattt
     4621 ggatcatggt tcttacatat cggatacatt aaaaattgat cccacaggat cacaactaga
     4681 agcgctcgtt gaaatttacc gcatgatgcg ccctggagag cctcctacaa aagaagctgc
     4741 tgaggcacta ttcaaaaacc tattctttgt tgaagaaaga tatgatctat ctgctgttgg
     4801 cagaatgaaa tttaatcgtc gcgtaggaat taaaagtgac gaaggcccag gcactttaac
     4861 gaaagaagac attttatctg tcattaaaac actgatagat attcgaaacg gaatagggat
     4921 ggttgatgat atcgatcatc taggaaatcg tagggtaaga agtgttggcg aaatgaccga
     4981 aaatcagttt agagtaggtc ttgttcgtgt tgaacgtgct gttaaagagc gcttaagttt
     5041 ggttgaatct gaaaatctga tgccacagga cttaattaac gcaaaaccag tatccgccgc
     5101 aattaaagag ttttttggat ccagtcagtt atcacagttt atggatcaag tgaatccatt
     5161 atctggtgtt acgcataaaa gacgtgtatc ggctcttggg ccaggtggtt taacacgtga
     5221 gcgagctgga tttgaagtcc gtgacgtaca tacaacacac tatggtcgcg tgtgtccaat
     5281 tgaaacgcct gaaggaccta atattggtct aatcaattca ctgtcagttt atgcaagaac
     5341 taacgaatat ggatttattg aaactccttg tcgaaaggtc gttaatgggc gtgtgacaga
     5401 tgaagttgaa tacctatcgg ctattgagga agttgatcaa tacattgcac aatccaatgt
     5461 tgagttagac gaacaaggaa atattcttgc tgacttagtt ccttgccgac atcaaaatga
     5521 attttcactg actactccgg ataaaattaa ctacatggat gtctcgccca agcaaattgt
     5581 gtcagttgct gcttcattga ttccgttttt agagcacgat gacgcaaaca gagcattaat
     5641 gggttcaaac atgcaacgac aggctgtacc aaccttgcgt tcagaaaagc ccttggttgg
     5701 gaccggtatg gaaagaatcg ttgcatctga ttcaggggtt tcagttgttg cgaagagagg
     5761 tggagttatt gatctggttg atgcttctcg tattgttgtt cgagtgaatg acgacgaaac
     5821 aacagcaggg gaaaccgggg tagatattta taacttaacc aagtatttcc gttctaacca
     5881 ggatacttgt atcaatcaaa gacccatagt atcaacaggt gatcgaattc aacgaggtga
     5941 tgtcctggct gacggtccat gtacagatat gggtgaattg gctcttggac agaatctatt
     6001 ggtggcgttt atgccttgga atggttataa ctttgaggat tccattctta tatctgaacg
     6061 aatcgttcat gatgacagat tcactactat tcacatcgaa gagttaacat gtatagctcg
     6121 agataccaaa ttaggtacag aagaaattac agcagatatt ccgaatgtag gcgagtctgc
     6181 actctccaat ttggatgaat ctggagttgt ttacatcggt gcagaagtaa aagcaggcga
     6241 tattcttgtg ggtaaagtaa ctcctaaagg agaaacccaa ctaaccccag aagaaaaact
     6301 gttaagagct atctttggtg aaaaagcttc agatgttaaa gattcatctt tgagagttcc
     6361 atcaggtatg aatggaacag tgatcgatgt acaagtcttt acccgtgatg gacttgaaaa
     6421 ggatgcccgt gctaaaagca ttgaagaaga gcatctagct cgagttcgca aggatttaat
     6481 agacgaacga cgtattcgtg aagaagacat ctatcataga gtttctcatt tgctgttgga
     6541 tagggtggca acaggcggtc ctggaagctt aaagccagga tctaaaatca cccaggatta
     6601 cttagataag gttgaaagag aaaagtggtt tgatattcgt atagaagatg acgcaattag
     6661 tcagcaacta gaacaattat ccaagcagct ggaactttta acaaaagaaa tggaaaagcg
     6721 ctttaacgac agtagaaaga aaattatcca gggagacgat ttagcacctg gtgtattaaa
     6781 aattgttaaa gtgtatttgg ctgttaaaag aagaattcag cctggagaca aaatggcagg
     6841 tcgtcatgga aacaagggtg tgatctccat tgttgttcca gttgaagata tgccacatat
     6901 ggaagatgga acggctgtag atatcgttct taatccattg ggcgtaccat cgcgaatgaa
     6961 tatcggccag gttcttgaaa cgcatctagg actagctgcc aaaggattag gaagaaaaat
     7021 tgcacagatg cttgatgaaa aacaaacacc tgaagcaata aaagcatatc ttgagaaaat
     7081 ttataatcat gacggagtgc agcgcgttaa tttgaaatgc ctcaatgatg atgaattgat
     7141 gacactggct gataacctga gagctggtgt gccgatggca actcccgtct ttgatggtgc
     7201 tactgagcaa gaaatcaaaa gtatgttgca gttagcagat ttgccagcag atggtaaaac
     7261 cgtgttgata gatggccgaa caggtaataa atttgataat ccggttactg taggctacat
     7321 gtatatgttg aagttaaacc atttggttga tgacaagatg catgctcgtt caactggatc
     7381 atacagtttg gtcactcaac aaccacttgg cggtaaagca caatttggtg gtcagcgttt
     7441 tggtgagatg gaagtatggg cattagaagc atatggtgca gcatacactt tacaagaaat
     7501 gttaaccgta aaatcagatg acgtcggagg tcgaacaaag atctataaaa atatagtcga
     7561 tggagatcat cgtatggacc caggaatgcc tgaatccttc aatgtattat tgaaggaaat
     7621 tagagcactg ggaattgata tcgagttgga gcatgactaa ctcatcagcg atacatactg
     7681 actaaccatt ttttggaggc atagacttgg ctaccctaag caacgaccca atgaaaaaag
     7741 caccagtaat gagtgactta cttggtattc ttaagcaaca aggtcaaagt gaagaatttg
     7801 atgcaataaa aattgcattg gcgtcacctg aattaattcg atcatggtcc tacggcgaag
     7861 ttaaaaagcc tgaaacaatc aactacagga catttaagcc tgaaagagat ggtttgtttt
     7921 gtgctaaaac ttttggtcca gtcaaagatt atgagtgttt gtgtggaaag tacaagcgac
     7981 taaagcatcg tggtgttatt tgcgagaagt gtggggttga attggctctt gccaaagtga
     8041 gacgagaaag aatggggcat atcgaactcg ctagcccagt cgctcacata tggttcttaa
     8101 aatctttacc atcaagaatt ggtttgctcc tcgatatgac actaagagat atcgagagag
     8161 tactatattt tgaagctttt gtcgttgttg atcctgggat gacagaactt gaaagagggc
     8221 aactacttaa cgacgaagcc tatcttgatg cgatggaaca gtatggtgat gagtttgacg
     8281 cgcgtatggg tgcagaagca attcgtgatt tattgcgcca aattgatctc gaagatgaaa
     8341 ttagaaattt acgtgaagaa cttccaacaa ctaactctga gacgaagatt aaaaaaatca
     8401 ccaaaagact caagttatta gaagcttttt acgagtcagg aaataaacca gaatggatga
     8461 tcatggatgt acttccagtg cttccacccg atttgagacc attggttccg ctagatggtg
     8521 gacgctttgc tacttcagac ttgaatgatt tatacaggcg cgtcattaat aggaacaacc
     8581 gactaaaacg ccttctggat ctcaatgcgc ctgacattat cgttcgtaac gaaaaacgaa
     8641 tgctgcaaga atctgtagat gctttacttg ataacggcag aagaggtcgt gccatcacag
     8701 gtacaaataa gagacctctt aaatcactgg cagatatgat taagggtaaa cagggtcgtt
     8761 tcaggcaaaa cctcttaggt aagcgtgtcg actactctgg acgttcagtt attgtcgttg
     8821 gaccaacttt aaaattgcac caatgcggat tgcctaaaaa aatggcgttg gagttattca
     8881 agccatttat attttccaaa ttggaattta gaggtttggc aactactatc aaagcggcca
     8941 agaaaatggt cgaaagagaa gaatcggtgg tttgggatat attagatgat gttattagag
     9001 aacatcctat ccttttaaac cgtgctccta ccttgcatag attaggtatt caagcatttg
     9061 agcctgtttt aattgaaggt aaagcgatcc agctccatcc tttggtatgt acagcataca
     9121 acgctgactt cgatggagac caaatggctg tgcacgtgcc cttgacactg gaagcgcagc
     9181 tggaagctcg ttcattgatg atgtctacaa acaatatatt atctccagcc agtggtgagc
     9241 cgattattgt tcccagtcag gacgtcgtcc ttgggttata ctatttaaca cgagaaaagg
     9301 ttaatgcatt aggcgaagga aaaatatatt caagtgctca ggaagcgcaa aatttctatg
     9361 aagcaggtca tttggatatc catgcaaaaa ttaaaattcg tatgcctaaa gaagacggtg
     9421 aaactggata tcatttagtt gagacaacag tcggacgcgc tattttagct gagattttgc
     9481 ctaaaggaat gccatttgac tatataaacc gtacaatgac aaaaaaggtt atctcaaaag
     9541 taatagacag ctgttaccgc aaattcggct tgaaagaaac agtcattttt gctgatcagc
     9601 taatgtatac cgggtttaaa tacgccacac gatctggggc atccataggt attgaagaca
     9661 tggaaatacc agatgataag gccagtatta ttgaacacgc tgataatgaa gtaagagaaa
     9721 tcgagtctca attcagatcg ggtttggtaa cgaatggtga acgatacaac aaggttattg
     9781 atatttggtc aaggactaat gaattagttg ccaaatcaat gatgtctaaa attgcaacag
     9841 aagaagttac tgatgcaaaa ggcaataaag taagacaaga atcatttaac ccaattttca
     9901 tgatggctga ttctggagct agaggttctg ctgctcagat acgacagctt gccgggatgc
     9961 gaggtctgat ggcagctcct gatggctcaa tcattgaaac gcctatcact gcaaacttcc
    10021 gcgaaggttt gaatgtattc cagtacttta tttctacaca cggagctcgt aagggtttgg
    10081 cagatacggc gttaaagaca gcaaactcag ggtatctgac acgacgcttg gtagatgttg
    10141 cgcaggatgt tgttattacc gaagatgatt gtggtactga tacaggtatt ctaatgcaac
    10201 cactgattga aggtggtgat attgttgagc ctttgcatga gcgcgtcctt gggcgggtgg
    10261 ttgcttcaga tgtctatatt cctactcaaa ctgaaccagt agtcaaggct ggaactttac
    10321 tggatgaaga atgggttgag aaattagaga agcatggagt agaccaggtt atggtcaggt
    10381 ccccaatcac ttgtcaaaca agatttggtc tttgtgcaaa atgctatggt agagatcttg
    10441 ctcgtgggca tcttgtcaac acaggtgaag cagttggtat tatcgcagca caatcaatcg
    10501 gtgagccagg aacacagtta accatgcgta ccttccacat tggaggcgct gcatcaaggg
    10561 caacagctgc gaataatatt caaattaaaa cgaaaggggt tatacggtta cataatataa
    10621 agaccgtaac tcatgagaat aaaaatctgg tggcagtttc tcgctcaggt gaggtaacta
    10681 ttgtcgatga gttcggacga gaaagagagc gatataaagt tccttatggt gcagtaattt
    10741 cagcgcagga taattcccct gttgaagcag gtcaggttat tgcaacgtgg gatcctcata
    10801 cacatcctgt tatttccgaa gtgtcagggc gtttgaagtt tgttgatctt attgatggca
    10861 ttacaatgaa tagacaaact gatgagttaa ccggattaag caatattgta ataattgatg
    10921 ccaaacaacg gagtgcggca ggtcgagatt tgcgcccaat ggtaaaatta gttacagatg
    10981 aaggagatga catatatctt gctggaacca atgtcccggc acaatattat ctacctgtcg
    11041 atgccattgt gaactttgaa gatggaagtc tggtgggaat aggagatgtt attgcccgta
    11101 ttccgcaaga acgttctaaa acccgagata ttaccggagg tttgccaaga gtagcggact
    11161 tatttgaagc cagaaaacca aaggattctg cagtaatggc agaagtgtca ggattggtta
    11221 attttggtaa agaaaccaaa ggtaagcgac gcttgattat caatgtatct gaagatcaat
    11281 gtcatgaaga gcttattcct aaatggcgtc atatttcagt gtttgaaggt gagcacgtag
    11341 agcgtggtga aatcatcgca gagggtgcac ttaaccctca tgatatactg cgcttgttag
    11401 gtgttggagc gctggctaat tacattgtaa atgaggtcca ggacgtctat cgtttacaag
    11461 gtgtgaaaat taatgataaa cacattgaag taattgtcag acaaatgcta cgtaagagag
    11521 ttattacttt cgctggtgac tcaaaattcc tcgtaggaga gcaagtagaa gagagtgcta
    11581 tgcttcagga aaatgataaa cttctagctg aaggaaagca gattgcgcga ggtactccta
    11641 ttttactagg tatcacaaaa gcttcattag ctacagaatc gtttatatca gccgcttcat
    11701 tccaagagac aaccagagtc ttgactgaag cagcagtaag cggaaaagtt gatgaattgc
    11761 gtggtttaaa agaaaacgtg atggtaggtc gcctaattcc agcaggaaca ggttacactt
    11821 atcatcaaag ccgaaaggca aaaagagcaa gggctgcagc aggcggagac agttcagcaa
    11881 cacacacagt tactgcaagt gatgttgaac acgcgttgag tgaagcatta aacgcggata
    11941 atcatgaaca ttaatcagga ttcaaaaatg gcagatttaa gcttgacaaa tctgccgtac
    12001 ctatatagaa tccggcctcc ttatgcattc aaaatattca caagtgacag atcggagtta
    12061 agtacaaatg gctactatta atcagttagt aagaaagcct cgtgtggacg taaagaagaa
    12121 aagcaacgtg ccagcacttg agtcatgtcc acaaagacgc ggtgtatgta ctcgtgtata
    12181 cactactaca ccaaaaaaac ctaactcagc tatgcgtaag gttgcacgtg tgcgcttgac
    12241 caatggattt gaagtaacat cctatattgg tggtgagggt cataatttac aagagcactc
    12301 tgttgtttta attagaggtg gtcgtgttaa ggatttacct ggtgtgcgct atcatacggt
    12361 tcgaggtagc ttagatactt caggagtgaa cgaccgaaaa caagggcgtt ccaagtatgg
    12421 tacaaaaaag ccaaaagata aaaaataacc gattgtagga aaattgagat gccaagaaga
    12481 cgagaagtac ccaaacgaga aattttacct gatccaaaac atcatagtga actgcttgct
    12541 aagttcatta acgttttaat ggttagcggt aaaaaatcaa ttgctgaaaa aatcacttat
    12601 ggtgcgctgt ctgttatgga agagcgtgtc aaaaaaatta agaaaaatga agaagatgga
    12661 agtgaaactg gcagcagcgg aagtgccggt gccgtacttc gttattttga agaggcttta
    12721 gataacgttc gcccaagcgt ggaagtacgt tcacgtcgag ttggtggtgc aacataccaa
    12781 gttccagtag aagttcgaca tgatagaagc atagcgcttg gaatgcggtg gatagttcaa
    12841 gctgctcgca cacgtggtga aaaaggcatg atgctgcgtt tggcagggga actgatggac
    12901 gcttatgaaa ataaagggtc tgcagtaaag aaacgtgaag atacacataa aatggcaaaa
    12961 gctaaccaag cctttgctca ttttagatgg aattaagaga gagataagcc gtggcaactc
    13021 cattaaaact atatagaaac ataggcattg cagcccacgt tgatgctgga aaaacaacaa
    13081 ctacagaacg agtattgtac tataccggaa tgtctcataa gattggtgag gtacatgatg
    13141 gtgctgcaac tatggactgg atggtccagg agcaggaacg tggtattacc attacttctg
    13201 ctgcaacaac atgttattgg tctggtatgg acaaacaatt tgaatcccat cggatcaata
    13261 taattgatac accaggacac gtagacttta tgattgaagt cgaacgctct ttacgtgtac
    13321 tagatggagc agttgttgta tttgattcag ttgccggcgt tgaacctcaa tctgaaacag
    13381 tatggcgtca agccaataag tatggtgttc ctcgtatcgt ttttgtaaat aaaatggata
    13441 ggatgggtgc caatttccta agagttgtta gccagattaa gcaaagattg ggctctacac
    13501 ctgttgtgtt acaattacca attggcgcag aagaagagtt caaaggtgtg attgatctca
    13561 ttaaaatgaa ggcaattcac tgggatgaag aaaataaagg tatgacattt aaatatgttg
    13621 atatccctgc tgatttaaag gcaacctgtg aagaataccg tgcacacatc attgaagctg
    13681 cggcagaata ctccgaagaa ttaatggaaa aatatttgga aggtgaggag ttcacagagg
    13741 cggaaattaa aaaggctctt cgtcatctga caatcacaaa taaagtagtt cctgtattct
    13801 gtgggtcagc ctttaaaaat aaaggcgttc aagcagtact ggatggagta atcgaatatt
    13861 tgccatcacc cactgatatt cctgatattc agggagttga tgagcatggt gatgaaatcc
    13921 atagaaaaac cagctatgat gaaccattct cagcgttagc gttcaaaatt gcgactgacc
    13981 catttgtggg cacactaaca tattttagag cttattctgg tattcttaag agtggggata
    14041 cagtctataa ctcagttaag ggcaaaaaag aacgtatcgg ccgcttgttg cagatgcatg
    14101 ctaattctcg tgaagaaatt aaagaagtgc gtgcagggga tatagctgct gctgttggtc
    14161 ttaaaacagt aaccactggt gataccctct gtgatcaaga taaagttgtt atattagaaa
    14221 gaatggattt tcccgatcca gttatagcag ttgctgtaga accaaaaact aaagcagatc
    14281 aggagaaaat gggaattgct ttaggcaagt tagcccagga agacccttct ttcagagttc
    14341 atactgatga agagtcaggt cagacaatca ttcaaggtat gggtgaatta caccttgaaa
    14401 ttattgttga ccgcatgaaa agagagttca atgtagaagc taatgttggt aaacctcagg
    14461 ttgcataccg agagacgctc aagcaagctg ttgaacaaga gggtaagttc gtaagacaat
    14521 caggtggacg tggtcaatat ggacatgtat ggttgaaaat tgaaccacaa gaaccaggaa
    14581 aaggttatga atttatcaat gctatcgttg gtggcgtcat tccaaaagaa tatatccctg
    14641 cagtagataa aggaattcaa gagcaaatgc aaaatggtgt aattgctgga tatccagtgg
    14701 ttgatgttaa agttacactg tttgatggtt cattccatga agttgattca agcgaaatgg
    14761 catttaaaat tgctggatct caatgcttca agcaaggtgc actaaaggcg aaaccggtac
    14821 ttcttgagcc aattatgagt gtcgaagttg ttacacccga ggattacatg ggcgatgtta
    14881 tgggtgatct gaacagacgc cgtggtttag ttcaaggtat ggaagactca ccagcaggca
    14941 aaatagttag agctgaggtg ccgcttgcag agatgtttgg ttattcaact gacttacgtt
    15001 ctgctacaca aggaagagca acctacacta tggagttttg taagtacgca gaagcaccaa
    15061 caaatattgc tgaagcaatt atcaagaaac aataaaagtt aacgaggtta ttgaaatggc
    15121 gaaggaaaaa tttgaacgta agaagccgca cgtaaacgtg ggcacgattg gtcacgtaga
    15181 ccatggtaag acgacattga cagcagctat tacaacgatt atggcgaaga aatatggtgg
    15241 tacagcgaag gcgtacgatc aaattgatgc tgcgccagaa gaaagagagc gtgggattac
    15301 tatttctaca gcacatgttg aatacgaatc agcgagcaga cattatgcgc acgtagactg
    15361 cccggggcat gctgactatg ttaagaacat gattactggt gctgcgcaaa tggacggagc
    15421 gatactggtt gtatcagc
//
LOCUS       DASLOE010000063        15359 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.063, whole genome shotgun sequence.
ACCESSION   DASLOE010000063 DASLOE010000000
VERSION     DASLOE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15359
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.063"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..596
                     /locus_tag="U6309_001540"
     CDS             <1..596
                     /locus_tag="U6309_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lpg1752 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504987.1"
                     /translation="EFQQNALKLTDQLKKRSIPSLGCFCFFSVILPNTAAIEDTHTIK
                     LVAYSLDSENDDDLIAYLKGVTRYSAVTPKNINEFKKKVLTGIYLMMWSHYSSTVSNL
                     MNSTLVELFQRDLQVSSPQKMSDTLFDASLEALSQYCSFAYQNRNERIYDNLIRRLGK
                     TVQADIHALRHSREKQETSFYDIYKGIMRTLGLNSVF"
     gene            609..1919
                     /locus_tag="U6309_001541"
     CDS             609..1919
                     /locus_tag="U6309_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1751 family Dot/Icm T4SS effector"
                     /protein_id="HEN5504988.1"
                     /translation="MTFTPPEFKILSVNTRNLETVFSTLLGRYKIITDPPVSEAVSSG
                     EIIRNTLETLLARTHKVVICKTDRETARDVFKQLSNELREVLKENNEEKNKQAILFLL
                     GALLHRYFRLIKEYDNFNSYIPYPSFLFSKPPSNVKDCRLFQAIRLALGLPEVMERNY
                     RINDLKILDVTTIVTALETFRDNMQLIIGKDEGKMPRYKSYPHFAADKNFETYLQEII
                     DEHKRRNPVVLNQFKAINFIQSLVKQIEEEQRQIEEALTHLGKLLPKTCSDFKTISLE
                     LMEEQIKAQIESNVLQEKIIDLLYTGHIQENFSTMDCGSFIEAMKNCNNSLARYRALG
                     GYCLLLQNEGVKEQLRFCIHQALGVEINPNELTDKDMLDAIRLLKTYFEANPKVELNF
                     DFFGGKGSMNTFILQTELALAKKVQTVNKAQEDNSETRTTSLFV"
     gene            complement(2011..4587)
                     /gene="clpB"
                     /locus_tag="U6309_001542"
     CDS             complement(2011..4587)
                     /gene="clpB"
                     /locus_tag="U6309_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEN5504989.1"
                     /translation="MRMDKLTSKFQMALADAQSLALGRDNGFIEPEHLMKALLDQQGG
                     SCRPLLSKAGVNIPLLRTLIDQALDKLPKVSGTGGDIHISNALNRLLNLTDKLSQQRK
                     DNFISSELFVLAAINEDSNLAKILKQAGGDNKAIEKAIDELRGGETVNDPNAEEQRQA
                     LEKYTLDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKNKRLLALDMGALIAGAKYRGEFEERLKGVLNDLAKQEGQIILF
                     IDELHTMVGAGKAEGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVDEPSVEDTIAILRGLKERYEVHHGVEITDPALVAAATLSHRYISDRQLPDKAID
                     LIDEAASLIRMEIDSKPESMDKLERRLIQLKIEREALKKENDEASKKRLVDLQKSIDE
                     LEQNYSDLEEIWKAEKATMQGSTQIKEALEQAKLEMETARRAGDLSRMSELQYGRIPE
                     LEKRLSQVSSVDAMETKLVRNKVTEDEIAEVVSKWTGIPVSKMMEGEKEKLLKMEEAL
                     HSRLIGQNEAVDAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALASFLFD
                     TEEAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HTDVFNILLQVMDDGRLTDGQGRTVDFRNTVIVMTSNLGSQLIQEMSSKFNYDEIKAA
                     VMDLVSQHFRPEFINRIDESVVFHSLTKEQIAKIAAIQINYLHHRLKQQNITLEVTSE
                     ALSHLAEAGFDPVYGARPLKRTIQQKLENPLAQSLLTGKFKSGDTIIVSYKDGVMEFS
                     KR"
     gene            4676..5632
                     /locus_tag="U6309_001543"
     CDS             4676..5632
                     /locus_tag="U6309_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEN5504990.1"
                     /translation="MNNESSSNSNLDSQALLNQLVIDYLQERKRKRRWKWIIRAIILV
                     VLVYSSYKIVSVAKTTEASKDKEHIGLIDINGEIADSSSASADNFTKGIEKAYKSKGL
                     KALIVRINSPGGSPVQAEYIYNVLQYYKSLKPNIKTYAVCVDMCASAAYYVAVGADEI
                     YASPASMVGSIGVLYNGFGFVDALQKLGISRRLQTSGVNKAFLDPFSPTTEFQKEKLQ
                     TMLDIVHKQFITRVKEGRGNRLHVDDETFSGLFWTGEQALANGLIDGYASSGQLAREV
                     IKITDIVDYTYKQNVFDRLTKNLGTAMADELPVILGMKPGLR"
     gene            complement(5745..6530)
                     /locus_tag="U6309_001544"
     CDS             complement(5745..6530)
                     /locus_tag="U6309_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019233089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEN5504991.1"
                     /translation="MEPLLNIAVNAARQAGEIINRYVEQVDRLKITPKNSHEYFSEVD
                     IKAEQVIINTIHKAYPEHGILAEESGIQQSDSDTVWIIDPLDGTSNYLHGFPFYSVSI
                     ALKIKNRLEHGVIYDPLRHECFAASRGRGARLNDRRIRVSKQTQLNASLLGTGFHFRD
                     ATLAQRYLPTFEALIGKCAGVRRTGSAALDLAYVASGRLDGFWEFGLRPWDIAAGALL
                     VREAGGLISDVQGGEDFLNYGDVVAGTPKVFKSLLQTISPVLK"
     gene            6646..7419
                     /gene="trmJ"
                     /locus_tag="U6309_001545"
     CDS             6646..7419
                     /gene="trmJ"
                     /locus_tag="U6309_001545"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HEN5504992.1"
                     /translation="MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPD
                     LKAKEMAAGADDVLDAAVVTNTLDEALIGCQLILGTSARPRGLSLPGLIPASCAELIN
                     QQSDNTQVAIVFGREHAGLTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMK
                     LLSPSAQVALRNEEPATADEIEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRIN
                     LEKMEVSILRGMLSQVQKSLEWARKDNRSNKIGNKTDLF"
     gene            7388..8557
                     /locus_tag="U6309_001546"
     CDS             7388..8557
                     /locus_tag="U6309_001546"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEN5504993.1"
                     /translation="MKLAIKPIYFDYMATTPVDPRVVEQMIKYLGPEGDFGNPASATH
                     EYGRVASMAVEQARSQIAETINASPQEIVFTSGATEADNLAILGAARFYKNKGMHLVT
                     MSTEHKAVLDSFHQLEKEGFQVTYLNPESDGLLDLGKLESALRLDTILVSVMHVNNEI
                     GVIQDIASIGELLRNRGIIFHVDAAQSAGKLPIDLSQLSVDLMSFSAHKNYGPKGVGA
                     LYVRHKPRIRLQPLSYGGGHEGGLRSGTLPTHQIVGMGEAFAIAEADRIPEQTRILNL
                     RKQLWDGIRHLPAIKLNGHEHRRIAGNLNVSFVGLNGDSLLFALSELAISTTSACSSA
                     SIQPSYVLRAIGLTDTEAQSTIRLSIGRFTSEVQIKKAIDIICTQVSRLHELSPL"
     gene            8554..8937
                     /locus_tag="U6309_001547"
     CDS             8554..8937
                     /locus_tag="U6309_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEN5504994.1"
                     /translation="MMYNKIVQDYFFQPQHIGKLDLNDPFVIGVQSQPINQLNTVHFY
                     IKCDGDKLISKARFKTNGNPYVIAAMEWICCQIEGKTLESLPLIDFQILINVLQITNN
                     QYPVAVQVEDIYKEALTLMRKKFER"
     gene            8942..9310
                     /locus_tag="U6309_001548"
     CDS             8942..9310
                     /locus_tag="U6309_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEN5504995.1"
                     /translation="MSVEIQQASSTNPNITFSESAIKHLVTYLEKNPGNKGVRLSVKK
                     TGCSGLSYVVDYVKEPSEGDMIASLSKDYLVCVDKTSYPFLKNMQVDYVKQGLNYKFI
                     FNNPNQTGQCGCGESFTVSQ"
     gene            9705..9992
                     /locus_tag="U6309_001549"
     CDS             9705..9992
                     /locus_tag="U6309_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEN5504996.1"
                     /translation="MSAVMQQVTQGNDALSHQVISAVKGYLTSVGSKDANLNLYQLIV
                     EEVEAPLFRTVMELTRYNQSKAARVLGVSRGTLRTKLKRYFDDEFIGTRDF"
     gene            10103..10475
                     /gene="rnpB"
                     /locus_tag="U6309_001550"
     ncRNA           10103..10475
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U6309_001550"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(10564..11370)
                     /locus_tag="U6309_001551"
     CDS             complement(10564..11370)
                     /locus_tag="U6309_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEN5504997.1"
                     /translation="MKKLIYISLIFVISNAFAQPAATVLFAKQKVISLREGKEYSLSR
                     GSQLEPGDTVVTGAGALINIKYKNGTLVNIGENSRYKILAFSPNPSDVQIKAELTQGK
                     IKFKTTGKSNESLKTPVIAMAITGTSAEIFVKSDKQTYLNLLEGNINNYAPGSYIATP
                     QGVVAGAFPAEGQVNTPAGTDGSISDAETTDTISSESADATDTADTTDTADAADTADA
                     TDTADSAQEASDAVGFIESAQVVDQATTDSIEEEIASEIAEISLECISVI"
     gene            complement(11390..11854)
                     /locus_tag="U6309_001552"
     CDS             complement(11390..11854)
                     /locus_tag="U6309_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5504998.1"
                     /translation="MKTIRLFMLSMIAHFLLTCSVIHNVWAEKTLNPEKLANTTITLI
                     SNNQNASIANCSLQSVTSCTILLHLANPGTPGILHVTNHSSFVTATNIEAVLPSSLEF
                     LVDMIESGCDVVPPGSTCSIYFYPVSGPISPQPVQLIGDNIRTATFYLGIEM"
     gene            complement(11894..14140)
                     /locus_tag="U6309_001553"
     CDS             complement(11894..14140)
                     /locus_tag="U6309_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEN5504999.1"
                     /translation="MNKLTLYFSQFLNHTKWTEFFLGLTVTLLVLLTLFFNIPPISSL
                     ISQFEGRIYDQIINLNWHSKPENLKVIIIDIDEKSLEKEGRWPWPRNKIAELLNKLQE
                     NGVVTVGFDIVMSEAETNYALGLIEKLSQFSGKMTTDQKQLIATLNNVANQVDNDQTL
                     VNALGKNNAILGFFFHNSEGIRKGALPTPLSDTDGNPLLANKLSVYQFTNFNGNLAAF
                     TAAATSGGFVTNLPDKDGVVRHALLLANYKNNLYPSLSLAIAMNYLLTDKVALKKYNN
                     QLTGIELDGLQIPTNRKGQILIPFWGGPGTLNYYSATDILNNQFNPEELAGSIALVGS
                     SITLLSDLHHTPVAQLFPGVEIVGNIVKGLVEQKIPREFDWESLQGKLCLVIFGLLFA
                     LLFSSIGIFGKLLIFLITTIAILTNTVILFVFYNNYIPPAFLLTITTLQTFVNFSYSY
                     VIEKRQKRRISELFGQYVPKEYVKELIEHPEHHTMEGLNREMTVLFTDIRNFTTISET
                     LGAGGVKLLLNSFFTPMTKIIFEHRGTIDKYVGDMIVAFWGAPIEDKEHASHAVLCSL
                     SCIEHLPQINTHLLEHGLPQVNIGVGIATGPMNVGDMGSEFRRAYTVLGDTVNLASRL
                     ESLTKFYQVNILVSDKTRSHLDSILWRTVDKVAVKGRKSPLTIYQPLGIVEQASARLL
                     QEQEKYETALAHYYNQDWLVAEILFNELKNVNPKIYLYQMYLERIAAFKKRGPSPDWD
                     GVYVHRSK"
     gene            complement(14339..15328)
                     /locus_tag="U6309_001554"
     CDS             complement(14339..15328)
                     /locus_tag="U6309_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEN5505000.1"
                     /translation="MKIKFLLSMIGVILSTQSAYAKIPDSSASKTQNINDNKSAKNTN
                     NFLKKITPEFAYSYIDFNFDSTAGLNFNRYNGHSNLYSIGADHFSPVPSIIAGLYVLN
                     IDTEVSSQFQLFPNTPIPSNQTIHNNTLFAHMRKIFTPKFDIDIAGGYGQNRINSQTL
                     LNRAIADIAFSKHTNDNWLASINAVYRKRGKKISLRTYVGMLYSQINSGSYILAFQSN
                     QRGQIIAPLTTKTTYIMEGTEISYKLNSTLTPFVNGGLIQIADFTNSRPILTQQINGS
                     LPQLNMDKNGYKLGAGLIFNYKQMTLRLEEKYYSAGNIFESYQTTAGLEYRFN"
BASE COUNT         4616 a         2817 c         3013 g         4913 t
ORIGIN      
        1 cagaatttca gcaaaatgca ttgaaattaa cagatcaatt aaagaaacgc agcataccat
       61 ccttaggctg tttttgtttt ttttcagtga tattacccaa tactgcagcc attgaagata
      121 ctcatacgat taaattggtt gcttatagcc tggatagcga gaatgacgat gacttaatcg
      181 cttatcttaa gggtgtcaca agatactctg ctgtaactcc aaaaaatatt aacgaattca
      241 aaaaaaaggt actaactgga atttatttaa tgatgtggtc acattatagc tctactgttt
      301 cgaatttaat gaatagcact ttggttgagc tatttcaaag agatttgcaa gtgagttccc
      361 cacaaaagat gagtgacact ttgtttgatg ccagtttaga ggctttaagt caatattgtt
      421 catttgccta tcaaaaccgt aatgaacgca tttatgacaa cttaattcgc cgtttaggta
      481 agacggtgca agcagatatt catgccttac gtcattccag ggagaaacag gaaacttcat
      541 tctatgatat ttataaagga attatgcgta ctttaggact taatagcgtg ttttagacag
      601 gagacactat gacttttact ccgcccgagt tcaaaatatt aagtgtaaat actcgtaact
      661 tagagacagt attctctacc ttgctaggca ggtataaaat tattactgat cctcctgtat
      721 ctgaagcggt atcgagtggt gaaataatta gaaatacttt agaaacatta ttagctcgga
      781 cccataaggt tgtgatttgc aaaacagata gagaaactgc cagagatgtt tttaaacaat
      841 taagtaatga attgcgagaa gttttaaaag aaaataatga ggaaaaaaat aagcaagcca
      901 tcctgttttt attaggagct ttgctgcatc gctattttag actgattaag gagtatgata
      961 actttaattc atatattcct tacccatcat ttttatttag taagccacca tctaatgtaa
     1021 aagactgtcg attatttcaa gcaataagac tcgctttagg tttacctgag gtgatggaga
     1081 ggaattatag aataaatgac ttgaaaattc ttgatgtaac aacgattgta actgctttgg
     1141 aaacatttcg tgataatatg caattgatta tagggaagga tgaggggaaa atgccgcgct
     1201 ataaaagcta tcctcatttt gcagcagaca agaattttga aacctattta caagaaatta
     1261 ttgatgaaca caaaagacgt aaccctgttg ttttaaatca attcaaggcc ataaacttta
     1321 ttcaatcttt ggtaaaacaa attgaagaag agcaacgtca aattgaagag gctttgactc
     1381 atttaggcaa actcttgcca aaaacctgtt ccgattttaa aaccatatcc ttggaactga
     1441 tggaagagca aataaaagct caaattgaat cgaacgtttt acaggaaaaa attattgatc
     1501 tgttgtatac cggacatatt caggaaaatt ttagcacgat ggattgtggt tcgtttattg
     1561 aagcaatgaa aaattgtaat aacagtcttg caagatatcg ggctttgggt ggttattgtt
     1621 tgttactaca aaacgagggc gtaaaggagc aattacggtt ttgcattcac caggctctgg
     1681 gtgtggaaat aaatcccaat gagttgactg ataaggatat gcttgacgca atcaggcttc
     1741 ttaaaacata ttttgaagct aatcctaaag ttgagcttaa ttttgatttt tttggtggaa
     1801 aaggatcaat gaatacattt attcttcaaa cagaattggc tttagctaaa aaagttcaaa
     1861 cagtaaacaa agctcaggaa gataattcag agactcgcac tacgtcttta tttgtttgat
     1921 gtgtttaccc agcttgctct tgaaacattg cctcgtaatt ttggagtcct ggataaggac
     1981 tcctgcgtta ttatccaatg ataagcatac tcatcgcttg gaaaattcca ttactccgtc
     2041 tttataagat acaataatgg tatctcctga tttaaatttt ccagtcaata atgattgagc
     2101 taagggattc tctaattttt gctgaatagt acgttttagg gggcgggcac catagaccgg
     2161 atcaaatcct gcttcagcca aatgagataa agcctcactt gtgacttcaa gagtgatgtt
     2221 ttgttgtttc agccgatgat gtaaatagtt aatctggatt gctgcaattt ttgcaatttg
     2281 ttcttttgtc agggaatgga ataccaccga ttcatcaatt ctgttgataa attcaggtct
     2341 gaaatgttgg ctgactaaat ccattaccgc agccttaatc tcatcgtaat taaatttgct
     2401 gctcatttcc tgaatgagtt gagatcccag attagaggtc ataacaataa cggtattcct
     2461 aaagtcaaca gtacgtccct gaccatctgt tagacggcca tcatccatca cttgaagcag
     2521 aatattaaat acatctgtat gagctttttc tacttcatca agtaagataa cagaataggg
     2581 cctgcgtctg actgcttcag ttaaatagcc accctcctca tatcctacat agccaggagg
     2641 tgctccgatt aagcgcgcaa cagagtgttt ttccataaat tcagacatat caatgcgcac
     2701 catcgcctct tccgtatcaa aaagaaaaga agcaagtgct ttacatagtt cagtttttcc
     2761 cacaccagtt ggccccagaa aaagaaatga accaattgga cgattaggat cagacaggcc
     2821 tgcgcgtgac cgtcttatgg cattagaaac agcatccacc gcttcattct ggccaatgag
     2881 tctactgtgc aaagcttctt ccattttcag cagtttttcc ttttctcctt ccatcatttt
     2941 agatactggg attcctgtcc attttgaaac aacttctgcg atctcatctt cggtgacttt
     3001 attgcgtacc aactttgttt ccatagcatc gacggaagag acctggctta atcttttttc
     3061 cagctcgggt atacgtccat attgtaattc ggacatacga cttaaatctc ctgctctcct
     3121 tgcagtttcc atttccagtt tggcttgctc aagtgcttct ttgatttgag tggatccttg
     3181 catggtcgct ttttcagctt tccaaatttc ttctaagtct gaataatttt gctctaattc
     3241 atcgatactt ttttgtaaat caacaagtct ttttttagac gcttcgtcat tttccttttt
     3301 caatgcttca cgctcgattt ttagctgaat caagcgtcgt tccagtttat ccatactttc
     3361 tggttttgaa tcaatttcca tccggattaa gctggcggct tcgtcaatta aatcaatggc
     3421 cttatcaggt agttgacggt cgctgatgta acgatgggat agcgttgctg cagcaaccaa
     3481 agcgggatct gtaatttcaa ctccatggtg aacttcgtaa cgttccttaa gtccccttaa
     3541 tatagcgata gtgtcttcca cactgggttc atcgaccagt actttttgaa agcgtcgttc
     3601 aagagcagca tccttttcaa tgtattgtcg gtattcgtct aatgtcgtag ccccaatgca
     3661 atgcaattca cctctagcca atgctggttt gagcatattc ccagcatcca tagctccttc
     3721 agccttgcca gcgccaacca tagtgtgtaa ttcatcaata aataatatga tttgaccctc
     3781 ttgttttgcc aaatcattaa gcacaccttt taaacgttct tcaaactctc cacgatattt
     3841 tgcacctgcg atcaaggcac ccatgtccag tgctagtaag cgtttattct ttaatccttc
     3901 aggtacttca ccattaataa ttcgttgtgc caaaccttct actatggctg ttttaccaac
     3961 accaggctca cctattaata cagggttatt tttggttctt ctttgcaata cctggatggt
     4021 gcgtctgatt tcatcatctc gtccaatcac tggatccaat tttccttgct cggcacgttc
     4081 agtcagatcc agcgtatatt tttcaagagc ttgtcgctgt tcctctgcat ttggatcatt
     4141 aaccgtttct ccacctctta actcatcaat cgctttttca attgctttgt tgtcaccccc
     4201 ggcttgtttg agtatttttg caaggttact atcctcattt atcgctgcta atacaaaaag
     4261 ctcgcttgaa ataaagttat cttttctttg ttgcgatagt ttgtcagtca ggttaagtag
     4321 tctattcaat gcattagata tatgaatatc tcctccggtg cctgatactt ttggtagttt
     4381 atccagagct tggtcaatta aagttcttaa tagtggtata ttaacacccg ctttactgag
     4441 aagaggtctg caacttccgc cttgctgatc aagcaaggct ttcattaaat gttctggttc
     4501 tataaatcca ttgtctctac ctaaagccag ggattgggca tctgcaagag ccatttgaaa
     4561 ctttgatgtt agtttgtcca ttcgcatagg aacacctttc tagttatttt agggtattaa
     4621 ttctagttta catgccataa aatgggtatt atttacattt tttcaagagt tttttatgaa
     4681 taatgagtca tcttctaatt ctaatttgga ttcacaagct ttgcttaatc aattggtaat
     4741 tgactatctc caagaaagga aaagaaagcg tcgatggaaa tggattataa gagctattat
     4801 cctggtcgta ttagtttatt catcttataa gattgtttca gtcgccaaaa ccacggaagc
     4861 atctaaggat aaagagcata ttggactaat tgatattaac ggtgaaattg ctgattccag
     4921 ttcagcaagt gctgataatt tcactaaagg aattgagaag gcttataaaa gtaaaggatt
     4981 aaaagcatta attgtacgga taaatagccc tggaggcagt cctgtacagg cggaatatat
     5041 ttataatgtt ttacaatatt ataaaagtct aaaaccaaac attaaaactt acgctgtttg
     5101 tgtcgatatg tgtgcttctg ctgcctatta tgttgcagtt ggagcagatg aaatctatgc
     5161 aagtcctgca agcatggtcg gctcaatagg tgttttatat aatggctttg gttttgttga
     5221 tgcattgcag aagcttggta tttcacgcag attacaaact tcaggagtca ataaagcttt
     5281 cctggaccct ttttccccaa cgactgaatt tcagaaagaa aagctacaaa ccatgcttga
     5341 tatagttcat aagcaattta ttactcgtgt caaagaagga agaggaaata gattacatgt
     5401 tgatgatgag actttttccg ggttattttg gactggcgag caagcgttgg ctaatggttt
     5461 aattgatggt tatgccagta gcgggcagtt ggcgcgagag gtaattaaaa ttactgatat
     5521 agttgactat acttacaagc aaaatgtctt tgaccgccta actaaaaatt tgggcacggc
     5581 gatggcagat gagttgccag taattttagg aatgaagcca ggattaagat aagagtggag
     5641 aggagtattt atccgctttc tcagcttcga gagagcggat attcgaaaat aatttaaaat
     5701 cgataagcct aaatgaacgt cttaaatgag cttaaattga caacttattt taaaacgggt
     5761 gatatggttt gcaaaagtga cttaaatact ttaggagttc ctgctactac gtcgccataa
     5821 tttaggaaat cttctccacc ctggacatca cttattaatc ctccggcctc tcgaaccagc
     5881 agagctcccg cagcaatatc ccacgggcgt aacccaaact cccagaagcc atccagacga
     5941 ccgcttgcaa cataagccaa gtcgagagca gcagaacctg ttcttctaac tccagcgcat
     6001 tttccaatca atgcttcaaa ggttggtaaa tatcgctggg caagagttgc atctcgaaaa
     6061 tgaaaacctg tgcctaacag agaggcattg agttgagttt gcttggacac acggattctt
     6121 ctatcgttta gccttgcccc gcgccctctg cttgcagcaa aacactcatg tcgcagggga
     6181 tcgtaaataa caccatgctc cagacgattt ttaattttca gggctatgga tacagaataa
     6241 aaagggaatc catgaagata atttgaggtg ccatccaatg gatcaatgat ccagactgta
     6301 tcactatcgc tttgctgaat gccgctttct tcagcaagaa ttccatgctc tggataggct
     6361 ttatgtatcg tattaatgat aacttgctcc gctttaatgt ccacttcact aaaatattca
     6421 tggctgtttt tgggagttat ttttaaacga tcaacttgct ctacataacg attaatgatt
     6481 tcaccagcct gtcgcgcagc attgacagca atatttaaaa gtggttccat ggatatactc
     6541 tttagctata aaatcagcaa ttctaacaca ttttttttaa acaaagagaa gttcaattaa
     6601 taaatcctat gatatagtta tctattgtat ttggaataat tttttatgaa gttaagttct
     6661 attcgaatcg ttttggtttc aacttcccat cctggcaata taggctctac tgcaagagct
     6721 atgaaaacca tggggctaag tacactgtac ctggttaatc caaagtcatt tcctgatctc
     6781 aaagcaaaag aaatggctgc tggagctgat gatgttctgg atgcagcggt tgttacaaat
     6841 accctggacg aagctttaat tggatgtcag ttgattttag ggacgagtgc cagaccccgt
     6901 ggactgtcat tacctggatt aattccagct tcctgtgcag aattgataaa tcaacaatca
     6961 gataatactc aagtggccat tgtatttggt agagaacacg caggccttac taatgaagag
     7021 ctgttaaaat gtcactatca tatcaatatc ccaagcaatc ctgagtacag ttcattaaat
     7081 ttggctcagg cagtacaaat tatcgcttat gaacttcgta tgaagttatt atcaccaagt
     7141 gctcaagtag ctctgcgcaa tgaagaaccg gctacagctg atgaaataga gcaattttat
     7201 gagcatttga aagaagtttt tattgaaatt aattttttaa agccctctaa tccaaggaga
     7261 ttaatgcaaa gagtgaggcg actgtttaat agaatcaacc ttgaaaaaat ggaagttagt
     7321 attttaagag gtatgcttag ccaggttcag aaatcattgg agtgggcaag gaaagacaat
     7381 aggagcaata aaattggcaa taaaaccgat ttattttgat tatatggcaa caacacccgt
     7441 tgatcctcgc gttgttgagc aaatgataaa atatttaggc cctgaagggg attttggtaa
     7501 tcctgcatca gcaactcatg agtatgggag agttgcctcc atggcagttg agcaggcacg
     7561 ctctcaaatt gcagaaacta ttaatgcctc accccaagaa atagtgttta catcaggagc
     7621 tacagaagcg gataatttgg cgattttagg agctgcccgg ttttataaaa ataagggaat
     7681 gcatctggtt actatgagca ctgaacacaa agctgtgctt gacagttttc accaactgga
     7741 aaaagaaggt tttcaagtca cctaccttaa tccggaatca gatggtttgt tggatcttgg
     7801 aaaactggaa tctgctctaa ggttagatac tattctggtt tctgtcatgc atgttaataa
     7861 tgaaattggt gtcatccaag atattgcatc tattggtgag ctattacgaa ataggggaat
     7921 tatatttcat gttgacgcgg ctcaaagtgc tggtaaattg ccaatcgatt taagccagct
     7981 ttcagttgat ttgatgtcat tctctgctca taaaaattat gggcctaaag gtgtaggggc
     8041 tctgtatgtc aggcataagc caaggattcg tttacagcct ttaagttacg gtggcgggca
     8101 tgagggtgga ttgcgctcag gaacattacc tacccatcaa attgtcggca tgggtgaggc
     8161 ttttgctatt gcagaggctg atagaattcc tgaacagacg cgtattctaa atttacgcaa
     8221 acaactatgg gacggaatac gacatttgcc tgctattaaa ctgaatggac atgagcacag
     8281 gcgtatcgct ggaaatctga atgtgagttt tgttggatta aatggagatt cattgctatt
     8341 tgctttaagt gagttagcga tatcaactac ttctgcctgt tcttctgcca gtattcaacc
     8401 atcttatgtc ttaagggcaa ttggtttgac tgatacagag gctcaatcaa ctattcgttt
     8461 atccatagga cgcttcactt ctgaggtgca aataaagaag gcaattgata taatttgcac
     8521 acaagtgagt cggctgcacg aattatcgcc gttatgatgt ataataaaat tgtacaggat
     8581 tatttttttc agccacaaca tattggtaag ctggatttaa atgatccttt tgtaataggt
     8641 gtacaaagtc aaccgattaa tcaactgaat acagtgcatt tttatataaa atgtgacgga
     8701 gataaactga tatccaaagc cagatttaaa acgaatggta acccttatgt tattgcggca
     8761 atggaatgga tatgttgcca gattgaaggt aagactttgg aatcacttcc attaatagat
     8821 tttcagattc taattaatgt attacaaata acaaataatc agtatcctgt agcagtccag
     8881 gtagaggata tatataaaga agcattgact ttgatgcgta agaaatttga gaggtaaagc
     8941 aatgagtgta gaaatacaac aagcgagcag tacgaacccc aacatcactt tcagtgaatc
     9001 ggcaataaag cacctggtca cctatctgga gaaaaaccca ggaaacaagg gagtaagatt
     9061 gtctgtaaaa aagacgggat gctcgggatt atcttatgta gtggattatg taaaagaacc
     9121 ttcagaagga gatatgatag cttctttatc taaagattat ctggtatgcg ttgataaaac
     9181 aagttatcca tttcttaaaa atatgcaagt tgattatgtg aaacaaggtt taaattataa
     9241 atttattttt aataatccca atcagacggg gcaatgtggt tgtggagaga gctttactgt
     9301 aagtcagtaa aataagcaga aaatctacga tgcttcttta atattatcaa taagcggctg
     9361 gcttgaaggg cagtcgcaat atccagcctc atggttttat attcttttta ttagttaaat
     9421 tactaatccc cgaaaaattt ctaattataa aagcagtgat tacttcattc atgtttaata
     9481 attattattt taaaagtaaa tttttagaaa aataaatgca attattttta caaacctaac
     9541 taaaatgata caataatgac ttaataattc cttaataaat gaatatcatt aaaacaatgt
     9601 ttacactctt tgggatattt gatattatat cggtcatggt gttagagcca aattaatgtg
     9661 ctggacatga tttatgttca ttaaatttgt attaggagat taaaatgagt gcagttatgc
     9721 aacaagtgac acaaggtaac gatgccttgt cacatcaagt gattagtgca gttaaaggtt
     9781 atttaacaag tgtcggtagt aaagatgcta atttaaactt atatcaattg attgttgagg
     9841 aagtagaagc ccctttattc cggactgtta tggaattaac tcgctataat caatcaaaag
     9901 ctgctcgagt gcttggtgta agtcgtggta ctttgcgcac taaattaaag cgttattttg
     9961 atgatgaatt cattggtact cgtgattttt aattcagtta tatagctaat actttccaag
    10021 ttcagttatt tttcgcggta ttctaataga aattcatcgt taattatggc ctttgcctgg
    10081 gcactctgct aatattgaag tcagagtcgg tcaggcaatc gctctttgcc taacagcaag
    10141 gggaggaaag tccgggctcc atagggcaga gtgccaggta actcctggga ggtgtgagcc
    10201 tacggaaagt gccacagaaa atataccgcc cacttttcct ttgggaatcg taggtaaggg
    10261 tgaaatggtg cggtaagagc gcaccgcgcg attggtaaca gtcgtggcag ggaaaacccc
    10321 actcggagca agaccaaata ggaatccatt tgactgcaag gtcctaacgt atggcccata
    10381 cgggattcgg gtaggtcgct tgaggcatgt ggtgacgcat gtcccagatg aatggttgcc
    10441 cacgacagaa cccggcttat cgaccgactc taatccatat tctatgttaa tgcataccat
    10501 tatatgcctt tggcccctat ataaataagg gggtaaagtc gcatacaaaa atgacatgcc
    10561 aagctatata acacttatac actccaggga gatctcggct atttcacttg caatttcttc
    10621 ttctattgaa tcagtagtcg cttgatccac tacttgagct gactcaataa atccaacggc
    10681 atcagacgcc tcttgagcgc tatcggcggt atccgttgca tcggcggtat ccgctgcgtc
    10741 agccgtgtct gtagtatctg ctgtatctgt agcatctgca gactctgagg agatagtatc
    10801 agtggtttca gcatcagaga tgcttccatc cgtgccagct ggagtgttta cctggccctc
    10861 agcaggaaag gcacctgcta caaccccctg tggcgtagca atgtaactcc caggagcata
    10921 attatttata tttccttcta gcaaatttaa ataagtttgt ttatcagatt ttacaaatat
    10981 ctcggcagat gtacctgtaa ttgccattgc tatcactgga gttttcagac tttcatttga
    11041 tttgcctgtg gttttaaatt ttatttttcc ttgagttaac tcagctttaa tttgaacatc
    11101 agatggattt ggggaaaagg ctagaatttt atatctggag ttctcaccta tatttaccaa
    11161 ggtcccattt ttatatttga tgttaatcag ggcgcctgcc cccgtaacaa cagtgtctcc
    11221 tggctctaat tgagagcctc gagagagtga gtattcttta ccttctctga gtgagatgac
    11281 tttttgtttg gcaaataata ctgttgctgc tggttgagca aacgcattgc ttataacaaa
    11341 aattagacta atataaatta attttttcat gagttcttcc tactggatat cacatctcta
    11401 taccaagata aaatgtcgca gttcttatat tgtcgccaat taactgaact ggttgcggag
    11461 aaatagggcc acttacagga tagaaataga ttgagcaggt acttccaggt ggaactacat
    11521 cacaaccgct ttcaatcata tccacaagaa actctagtga gcttgggagt acagcttcaa
    11581 tattggttgc tgtaacaaaa gaagagtggt tagtcacatg gagaattcct ggggtaccag
    11641 ggttagcaag atgaagaagt atggtacaac ttgtgactga ttgaagtgag caattggcaa
    11701 ttgaggcatt ttgattatta gaaattaatg tgattgtggt atttgccagt ttttcgggat
    11761 ttagggtttt ttcagcccaa acgttatgaa ttactgagca ggttaataaa aaatgtgcaa
    11821 tcattgataa cataaacagc ctaattgttt tcataatcct tcctattgca gtatttaaaa
    11881 tttcttcttg atctcacttg cttctatgaa catacacccc atcccagtca ggtgaaggcc
    11941 ctcttttttt gaatgccgct attctttcca aatacatttg gtacaaataa attttagggt
    12001 ttacattttt taactcgttg aagagaatct cagcgacaag ccaatcctga ttgtaatagt
    12061 gagctaatgc cgtttcgtat ttttcctgct cctgcagaag tcttgcagag gcttgttcaa
    12121 caatacctag aggttggtaa atagtcaatg gacttttacg acctttgacc gccactttgt
    12181 ctactgtacg ccataatatg ctatccaggt gagagcgagt tttgtcactt accaggatat
    12241 tcacttgata gaatttggtt agactttcta gcctggatgc gaggttaacg gtatcgccaa
    12301 gaacagtata ggctcgacga aactcagaac ccatgtcacc gacgttcata ggaccagttg
    12361 ctatacctac tccaatatta acttgtggca gtccatgttc caataaatgg gtattaattt
    12421 gaggcaagtg ctcaatgcag gaaagagaac acaaaacggc gtgacttgca tgctccttgt
    12481 cttctattgg tgctccccaa aatgctacta tcatgtcccc aacgtattta tcaatggttc
    12541 ctctatgctc aaaaatgatt ttggtcattg gggtgaaaaa tgaatttaac aacagtttta
    12601 caccgcctgc accaagtgtt tcgctgattg ttgtgaaatt acgtatgtct gtaaacagaa
    12661 ctgtcatttc acgattcaac ccttccatag tgtggtgttc gggatgctct attaattctt
    12721 tgacgtattc tttgggtacg tattgaccga acaattcact tattcgtctt ttttgcctct
    12781 tttcaatgac atatgaatag gaaaaattaa caaaagtttg caaggtagtg atggttaata
    12841 aaaatgcagg ggggatgtaa ttgttgtaaa atacgaacag gataactgta tttgttaata
    12901 ttgctatggt tgttattaaa aagattaata atttgccaaa aataccaata gaagaaaata
    12961 aaagcgcaaa taataagcca aaaatcacta aacaaagttt tccttgcaat gattcccagt
    13021 caaattctcg gggaattttt tgttccacca gtccttttac gatattaccc actatttcaa
    13081 ccccaggaaa taattgggca actggtgtat ggtgtaaatc agataagagt gttatggatg
    13141 agccaactaa ggctatactc ccagccaatt cctcgggatt gaattggttg ttcaatatgt
    13201 cagtcgctga atagtaattt agtgttcctg ggcctcccca aaatggaata agaatttgac
    13261 cttttctatt tgtggggatt tgtagcccat ctaattctat acctgtcaat tggttgttat
    13321 attttttcag agcaaccttg tcagttaaga ggtaattcat tgctattgcc aaagataaac
    13381 tgggatagag gttgttttta taattggcca ataaaagcgc atgtcttaca accccatctt
    13441 tgtcgggtaa atttgtaaca aacccgccag aagttgcagc cgcagtaaat gcggccaaat
    13501 taccgttgaa attagtaaat tgatacactg acagcttatt tgccaggagg ggattgccgt
    13561 cagtatcact taatggtgtc ggtaatgccc cttttctaat gccttcgcta ttatgaaaaa
    13621 aaaaaccaag tattgcatta tttttgccta aagcgttaac aagcgtttga tcattatcaa
    13681 cctgatttgc aacgttgttt aatgtggcaa tcaactgttt ttgatcagtt gtcatttttc
    13741 cagaaaattg actcagtttt tctatgaggc ccaaagcata attcgtttcc gcttctgaca
    13801 tgactatatc gaaaccaaca gtgaccacac cattttcttg taatttattt aataattcag
    13861 ctattttatt tcttggccat ggccatcttc cttctttttc taatgatttc tcatcaatgt
    13921 ctatgataat aactttcagg ttttcaggtt ttgaatgcca attaaggttg attatttgat
    13981 cataaattct tccttcaaat tgggatatta gtgaactaat cggtggaata ttaaagaaca
    14041 aggttagcaa taccaaaagg gttactgtca gccccaagaa aaactcagtc cactttgtgt
    14101 gattgaggaa ttgagagaaa taaagagtca atttattcac aaaaattatc cttttgctac
    14161 tcttaaatac aagatgaaag agcatcctct ttattaatca gtatatagtc tgagaggaaa
    14221 gggcagaaat aatgtttttc agccttgtag atcacaggtg tgtttgctcc atgttattac
    14281 aaaattcatt ttcgcgcttc tggtatgaag aacaataaat aaactactat aaaactaact
    14341 aattaaatcg gtactccagt ccggcagttg tttgataact ttcaaaaatg tttcctgcgc
    14401 tgtaatattt ttcttcaagc cttaaagtca tttgtttata attaaaaata agtccggcac
    14461 caagtttgta gccattctta tccatattga gctgaggcag agagccatta atttgttgag
    14521 taagtatagg tcgactattg gtgaagtccg ctatttgaat caaaccaccg ttaacgaaag
    14581 gggttaatgt tgaattgagt ttgtaactga tttcagtccc ttccataata taggttgttt
    14641 ttgtagttaa aggagctatg atttgcccgc gttggttgga ctggaaggct aatatataac
    14701 tacccgaatt aatttggctg tataacattc ccacataggt tcttagagat atttttttcc
    14761 ctcgttttct ataaactgca ttgatgctgg caagccagtt gtcattagta tgttttgaaa
    14821 aagcgatatc agcgatggca cgatttaaca aagtttgcga gtttattctg ttttgaccat
    14881 atcctccagc gatatctata tcaaattttg gggtaaatat tttacgcata tgtgcgaata
    14941 atgtgttgtt gtgaatcgtt tgattggaag gaattggagt attgggaaac aattgaaatt
    15001 gagaactcac ttcagtatct atgtttagaa cataaagccc tgcaatgatg ctcggtactg
    15061 gagaaaaatg atcagcacct atggaataaa gattggaatg cccattgtat cgattaaaat
    15121 taagaccagc tgtcgaatca aagttaaaat ctatatagct gtaagcaaat tcaggcgtga
    15181 ttttctttaa aaagttatta gtattttttg cgctcttatt gtcatttata ttttgtgttt
    15241 ttgatgctga gctatcagga attttagcat aggctgattg tgtgctcagt attactccaa
    15301 tcattgataa caaaaacttt attttcatta tttcaaaaat ccttagcgaa atgacaaat
//
LOCUS       DASLOE010000064        15192 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.064, whole genome shotgun sequence.
ACCESSION   DASLOE010000064 DASLOE010000000
VERSION     DASLOE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 15192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15192
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.064"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            144..1715
                     /locus_tag="U6309_001555"
     CDS             144..1715
                     /locus_tag="U6309_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEN5505001.1"
                     /translation="MRRQWYIGFGVILLCLFALFAMLQDIVWFVAFLIPIVILWIYDV
                     LQTKHTILRNFPVLGHVRYFLEFLRPEIQQYFIATDESELPFNRETRSMIYQRAKNVR
                     DTIPFGTERDILSVGYTWALHSLSPKHSSEVEPRIDVGGPDCKKPYNASRLNISAMSF
                     GSLSSNAIMALNKGAKLGGFYHNTGEGGLSPYHLQGGDIVFQIGTAYFGCRDDKGDFD
                     ENEFIKEASRDEVKMIEIKLSQGAKPSHGGILPAAKLTEEIAKVRKVPMGKDVLSPIA
                     HTAFDNPVGLLHFVKRLRDLSGGKPIGFKLCLGRRHEFMAICKAMLKTNILPDFITID
                     GAEGGTGAAPVEYTNFIGTPLEAGLVFVHNALVGAGVRDKIRVICSGKVTNGFDLLTN
                     IALGADICNSARAMMMAIGCIQSKQCNANTCPTGVATQKKRLQYGLVVDQKKFLVANF
                     HNNTIKSFLEMVGALGLNNPSDLKPSHIMRRIGVDEVKSFDQIYTYVKPGQFLQGDIP
                     DDYRIHWEHADPDKF"
     gene            complement(1807..3420)
                     /locus_tag="U6309_001556"
     CDS             complement(1807..3420)
                     /locus_tag="U6309_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEN5505002.1"
                     /translation="MNIKSNLAHSSVILKGHVNRYAFLGLLISVGSILIASCIVSYQL
                     TGSVSLGGFIQAQRSNPAIWVLDLTPFMFVYWGQAFCYGLVNKAESLLVDKTKELLNI
                     SGNLELKLKYESNHDSLTNLPNNRLLNEKIRLAIEQLGSQGELAVIVIKINDLNYNFS
                     SFNANNIVKQFAEKLKSILIDPYMLKASLGINMVARLQSDEFAILMPRLKKDLDINEL
                     LIHLLTLLNNNLMVDGISINVTSTVGAAIYPVHGEEDDVLVNHAIIAVYQARKENKSY
                     AIYSPEMEEDLIQNRMVMSELQHSIENKDLKIYYQPIVELANGQIVGAESSARFEHPE
                     LGLLSAEKFMPLIEGTNLIHNLTILMLKEVIKQLAAWHDAGHKIFVSVNLSVHHELPE
                     LIEKLLNDYEIAPQFLKLEFTERACLTEQMVTKEVFDRLSTMGIKLCIDDFCSENSSF
                     IHLTNFPIENIKIEKSFVLKIAKDAKKAKIVEAIVKLAQTLGWEPLADGIADQNALEK
                     LRDLGCLYGQGLYFSRAVNVAEFTSLLKK"
     gene            3618..3780
                     /gene="ssrS"
                     /locus_tag="U6309_001557"
     ncRNA           3618..3780
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U6309_001557"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3775..5766)
                     /gene="uvrB"
                     /locus_tag="U6309_001558"
     CDS             complement(3775..5766)
                     /gene="uvrB"
                     /locus_tag="U6309_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEN5505003.1"
                     /translation="MKDLFKIYSNYQPAGDQPTAIASLIDGLESGLAKQTLLGVTGSG
                     KTFTIAHVIQAMKRPTLIMAPNKTLAAQLYGEFKAFFPDNAVEYFVSYYDYYQPEAYV
                     PASDTFIEKDASINEHIEQMRLSATKALIERKDAIIVATVSAIYGLGDPDSYLRMLLH
                     LSRGEQSDQRKILKRLAEMQYTRTNLSLERGQFRVHGDVIDIFPADSEKEAIRIELFD
                     DEVDNIARFDPLTGEILQRLPRVTIFPKTHYVTPRERILETVEKIKVELQERLAELNA
                     QNKLVEAQRLEQRTCFDIEMMLELGYCSGIENYSRYLSNREAGEAPPTLFDYLPPEAL
                     LIIDESHVTVPQIGGMYRGDRARKETLVNYGFRLPSALDNRPLRFEEFEERSPQTIYI
                     SATPGPYEQEHSDNVAEQVVRPTGLIDPEVEIRPVKTQVDDLMSEIRQVIAQGSRILV
                     TTLTKRMAEDLTEYLSEHGIKVRYLHSDVDTVERMEIIRDLRLGEFDVLVGINLLREG
                     LDMPEVALVAILDADKEGFLRSERSLIQTIGRAARNVKGRAILYADTMTGSMQRALTE
                     TERRREKQKAFNLKHGITPKGINKSVEDILEGAYIGKRKTMVAEQAPRYTHWSPQELA
                     KQINALEKQMYAHAQNMEFELAAKIRDEYLLLKEQLMKI"
     gene            5813..6991
                     /locus_tag="U6309_001559"
     CDS             5813..6991
                     /locus_tag="U6309_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN5505004.1"
                     /translation="MDIALAKRVQKVKPSPTLAVAAKAAQMKAQGLDIIGLGTGEPDF
                     DTPQHIKLAAISAIEAGDTKYTAVDGIVELKEAVKNKFKRDNELDYQLNQILVSVGGK
                     QSCYNLCQAYLNPGDEVIIPAPYWVSYPDMVLLADGVPVIIETTPAQRYKINAQQLEQ
                     AITPKTRMIFLNSPSNPSGIAYTQNELKELGDVLKKHPQILIATDDMYEHIIWSQPFM
                     NILNACPELYDRTIVLNGVSKAYAMTGWRIGYAAGPAPLINAMKTIQSQSTSNPCSIA
                     QRAAVAALNGSNESIEEMVNAFHQRHDYVADRLQSIDGIEVIPADGTFYIFPSVQAII
                     EKRGYANDIEFSEKLLNEVGVALVPGSAFGTEGCIRISFATGIDTLKDALNRLQRFCS
                     "
     gene            7051..7126
                     /locus_tag="U6309_001560"
     tRNA            7051..7126
                     /locus_tag="U6309_001560"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7084..7086,aa:Asn,seq:gtt)
     gene            7236..7844
                     /locus_tag="U6309_001561"
     CDS             7236..7844
                     /locus_tag="U6309_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505005.1"
                     /translation="MDASKGNNHLKSLNKYSWFILVMFIFAVFAMSYQTTNTFFDGFI
                     QTLPLIIVFVFWSEKSARLIKQAESNLKRAELFNRDTFILSFSFLLGCLISLLFAYDN
                     SDVKGWWVLIIYFITLYGLIFSLIFSGIALQIKNHKTYTLVFSLLIIVFVSMGKFFPR
                     YTFIPLLGYIDTFYAVTCVLLIIHCLFAFNCKIIRAIKRNTP"
     gene            7875..9287
                     /locus_tag="U6309_001562"
     CDS             7875..9287
                     /locus_tag="U6309_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505006.1"
                     /translation="MKSKKILILGLSITLTAITSASYAKKIFPSDIIGRDLNYPGMGW
                     LGHVGIATRPMMDSSGMSTPANQVIEILNEPVIGQINTIDNFKRRSPYWGSKYGVIGN
                     PEQGYRVLVEANHQRWWVREYTSNTDYRIAQGNPRTGEIFVTGRWRCDTYAWWAFYSQ
                     GVDTMPGRIWLPRNLFNFFPYYNDERLVAESSEPSAEILTNKSLDDVSAEDLNEMSLE
                     EFQTVIDAPLKAPTNYVAPMSAYMRFAYSDSLNDIKRGAMIDKITSKVSDVDLVPKLL
                     RLYDETDREEVKNKIVSGLMFHVQGELREHPNSRDKELLRSFFHRLIGEKLNQRCADW
                     ATMGFIDTHTADETLQNLDKIDAQLATVTHPASITLKYKLVHKSKKLQSIYIKSIVDE
                     LREANNSDLDSYFFGPLAIGYQGTGKNLLEPESKQIVINYLDEVRYKYTSKGIKANPG
                     DFHRGTTAPYYFELLKHMDN"
     gene            9692..9916
                     /locus_tag="U6309_001563"
     CDS             9692..9916
                     /locus_tag="U6309_001563"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505007.1"
                     /translation="MTEIPRWFSLLNVPPRGYGMKWLSIRHQLMFRQGFTSASVMLDS
                     KLAKRCLLSKGFWKGFCPIPIGGIGQNAKM"
     gene            complement(9982..10431)
                     /locus_tag="U6309_001564"
     CDS             complement(9982..10431)
                     /locus_tag="U6309_001564"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505008.1"
                     /translation="MLNWITLHQRRPYRSRTFIPTHEDTLESGESCNFRQKPNIANYT
                     EADPPQKSEDYNYIYMCSKFEKFWKSYPHPVDREAAFYQFRELNPDDTLFEKIMTGLH
                     GQIESYKIQVMLGSWVPKWKYPANWLAKKCWQTDPYVFEHIVYDMEN"
     gene            complement(10364..10728)
                     /locus_tag="U6309_001565"
                     /pseudo
     CDS             complement(10364..10728)
                     /locus_tag="U6309_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein LvrA"
     gene            11095..11334
                     /locus_tag="U6309_001566"
     CDS             11095..11334
                     /locus_tag="U6309_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN5505009.1"
                     /translation="MIISSAKDLALYLNDKRKQLKMSQSEVADLVGLKQDTISKFENS
                     PDNSRIDTLFRILSALNLNMSLVEKDQKTHEEIEW"
     gene            11328..12632
                     /locus_tag="U6309_001567"
     CDS             11328..12632
                     /locus_tag="U6309_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEN5505010.1"
                     /translation="MVMRKLYILMNNELVGELTQDNAGGLSFSYVADWISKPHSRPVS
                     LSLPLVNQKFSGEVVYNFFDNLLPDNPQIRARIQARFQAKSSRPFDLLALIGSDCVGA
                     IQIVERIPQTQRLSIESHVVSEAEIARTLRSYQTSPLGMDKEDDEFRISIAGAQEKTA
                     FLYYQNEWHKPFGPTPTTHIFKLPIGYLPHQGIDLSESCENEHLCLLLASKFGLDVAK
                     SKILQFEDEKVLVVERFDRKWSRDNRYILRLPQEDLCQALGYSPNLKYQSDGGPGIKE
                     IMQLLNGSQQPQKDRDEFFRAQVVYWCLGAIDAHAKNFSISIQPQGGYQLTPLYDIIS
                     AYPIVEQKQLSFKKLKMAMALYGKNIHYKIDGIQKRHFIETAKYVKYSKIKAAQIFEQ
                     VIDRIDSAIDEVSRQLPNHFPEKIADPIFKGMRAFKERMIST"
     gene            complement(12702..12977)
                     /locus_tag="U6309_001568"
                     /pseudo
     CDS             complement(12702..12977)
                     /locus_tag="U6309_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634034.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(13019..14110)
                     /locus_tag="U6309_001569"
     CDS             complement(13019..14110)
                     /locus_tag="U6309_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505011.1"
                     /translation="MNKEIKKNLQNNNSAFVYLRSTGLATNSLSSILQYMTRIIVLTE
                     SAGIALGILGVLFSVIGFVSSTIARILDPTASKLSRVMSAGILIAAAAGAIPLIYLGT
                     LFPAMWVYLGIGIMGFGVLNSSYYLYSDVRKENALINQYKDNPEQYVESIKEKRQAKY
                     EKLLNTRLDDKQARVLLVFKLYQLDKKLAVAKQEGLARLYDIVCHRKHTRSLERFIDL
                     GTSLTSLIVGVSGLFWGIAAWPIGPILLGISLAVFSVSLSKSAMQFYRKVNKNNALLK
                     EVLSNDRYISLIDANLDNPYDIEEMGTVEKTQEKVCTHELSQESALSKDQEHKEHYTK
                     LFQPVNKTALRPNDEETDELSCKSVLLQR"
     gene            complement(14300..15052)
                     /locus_tag="U6309_001570"
     CDS             complement(14300..15052)
                     /locus_tag="U6309_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEN5505012.1"
                     /translation="MNHTSIKKAVLYRMVIEHHVCPYGLKARDLLRRQGYEVEDNWLT
                     TRDQVDAFKDKHNVKTTPQIFINDERIGGYDDLRRYFGKKVRDPKEKSYRPVIVLFTI
                     TALMAMAVSYASFANPFTPHVLSWFIAFSMAVLALLKLQNIESFSTMFLNYDLLAKRW
                     VPYSYIYPFAEALAGILMIADALNWLSIPIALFIGTVGALSVFKAVYIDKRELKCACV
                     GGDSNVPLGFISLTENLMMIGMALFMLLMSFN"
BASE COUNT         4632 a         3099 c         2925 g         4536 t
ORIGIN      
        1 atatcttctt tggaaaataa aattaataaa gcagtagttg gctctgaaat ggatagcaat
       61 caataagcag ataaattaag tattcctcgg taaaccatcc tatactattg atattaatag
      121 caaacaatta aaaggaatta gctatgcgac gtcaatggta tatcggattt ggagttatcc
      181 ttttatgtct ctttgccctg tttgccatgc tgcaggatat tgtctggttt gttgcctttt
      241 tgatacctat tgtaatcctg tggatttatg atgtattgca aacaaaacac accattttac
      301 gcaattttcc agtactaggt cacgttagat attttttgga atttcttcgc cctgagattc
      361 aacaatattt catcgctacc gatgaaagcg aacttccttt taacagggaa acccgctcca
      421 tgatttatca acgcgcaaaa aatgtcagag acaccattcc ttttggaacg gagcgagaca
      481 ttctaagcgt tggttatact tgggcactcc attctttatc gccgaaacac tctagcgagg
      541 tggaaccaag aatcgatgtg ggtgggcctg attgtaaaaa accttataat gcatcccgat
      601 tgaacatatc agccatgagc tttggctctt tatcctctaa tgccataatg gcattaaaca
      661 agggagcaaa acttggagga ttttaccaca atacagggga agggggattg agcccttacc
      721 atttgcaagg cggtgacatt gtttttcaaa ttggcacagc ttattttgga tgccgtgatg
      781 acaaaggcga ctttgacgaa aatgaattta tcaaagaagc atcccgtgat gaagtaaaaa
      841 tgatagaaat taaattatca caaggcgcga agccatctca tggtggaatt ttgcctgctg
      901 ccaaattaac tgaagaaatc gcaaaagtaa gaaaagtacc catgggcaaa gatgttttat
      961 cgccaattgc ccataccgct tttgataatc ccgttggttt acttcatttt gtaaaacgat
     1021 tacgcgatct ttcaggtgga aaacccatag gctttaaact ctgccttggg cgccgacatg
     1081 aatttatggc tatttgcaaa gccatgttga aaacaaatat tttacctgac tttattacaa
     1141 tagatggtgc cgaaggaggg acaggagcag caccggttga atacaccaat tttataggaa
     1201 ctcccttgga agccggcctg gtttttgttc ataatgccct tgttggcgcc ggtgtacgcg
     1261 ataaaattcg cgtaatttgc agtggaaaag taaccaacgg gtttgatttg ctcactaata
     1321 ttgcccttgg ggccgatatc tgcaattcag caagagccat gatgatggca atagggtgca
     1381 tacaatcaaa acaatgcaat gccaatacct gcccgacagg agttgccact caaaagaaac
     1441 gtcttcagta tggtcttgtt gttgatcaaa aaaaatttct tgttgctaat ttccataata
     1501 atacgataaa aagctttttg gaaatggttg gcgccttggg attaaacaat cccagtgatt
     1561 taaaaccatc ccatattatg agacgaatcg gggtggatga agtcaaatct tttgatcaaa
     1621 tctatactta cgttaagcca ggccaatttt tacaagggga tattcctgat gattatagaa
     1681 tccactggga gcatgccgat ccagacaagt tttaatatca gtgtggccat agcttgtcag
     1741 cgttgtgatt cgtattccat gtgaatcaaa gcgggaaaac gaatggtaag tgatttaaag
     1801 ccagattcat ttctttagca atgatgtaaa ttcagccaca ttgaccgctc gagaaaaata
     1861 taatccctga ccatacaagc aacctaagtc tcttaatttt tccaatgcat tttgatcagc
     1921 gatgccatct gccaggggtt cccaccctaa tgtttgggct aactttacta tagcctctac
     1981 aattttggct ttctttgcgt cttttgcaat tttcagtacg aatgatttct caattttaat
     2041 gttttcgata gggaaattag tcaaatgaat gaaggaagag ttctcactgc aaaaatcatc
     2101 aatgcataat ttgatgccca tggttgataa tcggtcaaac acttccttgg taaccatctg
     2161 ctcagttaaa caagcccttt ctgtaaattc aagtttcaag aattgcgggg caatctcata
     2221 atcatttaat aatttctcta ttaattcagg caactcatgg tgcactgaca gatttacaga
     2281 aacaaaaatt ttatgtccag catcatgcca agcggctaat tgctttatga cttccttaag
     2341 cataagaatt gtcaaattat gaattaaatt ggttccctcg attaaaggca tgaatttttc
     2401 tgcacttaat aaacccaatt caggatgttc aaaacgagct gatgactcgg cacctacaat
     2461 ttggccatta gctagctcaa ctattggctg gtaataaatt ttcaaatctt tgttttcaat
     2521 ggagtgttgc agttcactca tcaccatccg attctgtata aggtcctctt ccatttcagg
     2581 gctgtaaatt gcataggatt tgttttcctt tcgtgcctgg taaactgcaa taatggcatg
     2641 atttactaaa acatcgtctt cctctccatg gacaggatag atagcggcac caactgtaga
     2701 tgtcacattg atactaattc catccaccat caggttatta ttcagcaagg taagaagatg
     2761 gattaatagc tcattaatat ccaaatcttt tttaagtctc ggcatcaata tggcaaattc
     2821 atcactttgc aatcttgcaa ccatattaat tccaagagaa gcttttagca tgtagggatc
     2881 aatcaaaatc gattttaatt tttcagcaaa ttgctttacg atattattag cattaaatga
     2941 gctgaaatta taattaagat cattaatttt tatcacaata acagctaatt caccctgtga
     3001 acccaattgt tcgatggcga gccgtatttt ttcatttaac aagcggttgt taggcaaatt
     3061 ggtcaagcta tcgtgattac tttcatattt aagctttaat tccagatttc cactgatatt
     3121 taaaagctct ttggttttat caaccagtaa actctccgct ttgttaacca atccataaca
     3181 aaacgcttgc ccccaataca caaacataaa aggtgttaaa tccaggaccc aaattgccgg
     3241 gttagatctt tgagcctgaa taaacccgcc taaactcaca gatccagtca attgatagga
     3301 aacgatacaa cttgcaatca agatactgcc aacagaaatc agcaatccta aaaaggcata
     3361 tcgattaaca tgacctttca ggataacaga gctatgtgcc aaattagatt taatattcat
     3421 ggattaacag taattccctt atgcctgtca acaagctaat ttttaattcc ccatcccttc
     3481 tacagcgact tcaatgctct acaaaaaatt gaataaatgg actacttaat gattagtata
     3541 gcctaaagaa aaaacaacaa atgctctatc gtatggagtt tttctggatg taaaaaccag
     3601 gttaatgtta taatctaatt aacccgatgg cgagtgattt tagaaccaac actcacagcc
     3661 agggaagcat cctgttatcg gcgttgagca agcccaccaa tagagtggga agcctgaacc
     3721 gatgcattcg ctgcccactt gaacctaagg gttcaaaatc cggtacccat cgggttatat
     3781 cttcatgagc tgctctttta atagcaggta ttcatcccga attttagctg ccaattcaaa
     3841 ttccatattt tgagcatgag catacatttg tttttccagt gcattgatct gttttgccaa
     3901 ttcctgagga gaccaatggg tatatcgggg agcttgttca gcaaccattg tctttcgttt
     3961 accgatatag gcaccctcca aaatatcctc aactgattta ttaataccct tgggagtaat
     4021 gccatgtttc aaattgaatg ctttttgttt ttcacgtcgt ctctccgttt ccgtcaatgc
     4081 tctttgcatc gaaccggtca tggtatcggc atacaaaatg gctcgcccct tcacattacg
     4141 cgctgcacgg ccaatcgttt gaattaaaga acgctccgat cgcaaaaacc cctccttgtc
     4201 agcgtccaaa atcgcaacaa gagcaacctc gggcatatca agaccttcac gcaataaatt
     4261 aatccctacc agaacatcaa actcacccaa acgcaaatca cgaataattt ccatgcgctc
     4321 aaccgtatcg acgtcggaat gcaaatagcg caccttgatc ccgtgctcac tcagatactc
     4381 tgtcaaatcc tctgccatgc gtttggttaa tgtcgtgaca agaatacggc tgccctgagc
     4441 gatcacctgt ctgatttcag acataagatc atccacctga gttttcaccg gcctgatttc
     4501 cacctcagga tcaatcaatc cggtcggtct taccacttgt tcagccacat tatccgaatg
     4561 ttcctgttca taaggccctg gagttgctga aatataaata gtctgtggcg aacgctcttc
     4621 aaattcttca aaacgcaaag gcctattatc caaagcagaa ggcaggcgaa atccataatt
     4681 aaccaaggtt tccttgcgcg ccctgtctcc gcgatacatt ccacctattt gaggaacagt
     4741 aacatgcgat tcgtcgataa ttagcaaggc ctccggcggg agataatcaa aaagagtagg
     4801 cggtgcttcc ccggcttcgc ggttggataa ataacgcgag taattttcaa tgccagagca
     4861 ataacccaac tccaacatca tttcaatatc aaaacaagtc ctttgttcca aacgctgagc
     4921 ttctactaac ttattttgag cattcaactc agccagtctt tcttgtagtt ctacttttat
     4981 tttttctact gtttctaaaa tacgttctct gggagtaaca taatgagttt taggaaaaat
     5041 agtgacacga ggcaaccgct gtaaaatttc tcctgttaaa ggatcaaagc gggcaatatt
     5101 atcaacctca tcgtcaaaaa gctcaattct tattgcttct ttctcggaat cagccgggaa
     5161 aatatcaatg acatcaccat gtacccggaa ttgaccgcgc tcaaggctga gattggttcg
     5221 tgtgtactgc atttctgcaa gacgctttaa aattttacgc tgatcggatt gttctccacg
     5281 ggagagatgt aataacatac gcagatagga atccgggtcg cccaatccat aaatggcaga
     5341 aaccgttgct acaatgatgg cgtctttgcg ttcaatcaac gctttggttg ccgacaagcg
     5401 catctgctct atatgttcgt taatggaggc atccttttct ataaaagtat ctgaagcagg
     5461 cacatacgct tcgggctgat agtaatcgta gtaggaaacg aaatattcta cagcattatc
     5521 aggaaagaag gctttaaact ccccataaag ttgagccgca agcgttttgt tcggcgccat
     5581 gatcaaagtg ggtcttttca tggcttgaat aacatgagct atagtaaatg ttttaccaga
     5641 accagtaacg cctaataatg tttgtttggc taaacctgat tcaagaccgt caatcaaaga
     5701 ggctatggct gtaggttgat cgccggcagg ttggtaattt gagtaaattt taaataagtc
     5761 tttcatattt tatattatat gctagtaccc ctataattca caggagcaac agatggatat
     5821 cgcattggca aagcgtgtac aaaaagtaaa accttcacct acccttgccg tagctgcaaa
     5881 agcagcgcag atgaaagccc aggggcttga tatcattggc cttggaactg gtgaacctga
     5941 ttttgacacg ccgcagcata taaagctagc cgccatctct gctattgaag caggtgatac
     6001 caaatacacc gccgttgatg ggatagtcga actaaaagaa gcggttaaaa ataaattcaa
     6061 aagagacaat gaactggatt atcaactcaa tcagatattg gtctccgtag ggggtaaaca
     6121 aagctgctat aacctctgcc aagcttacct taaccctggc gatgaagtca tcatcccagc
     6181 tccttattgg gtatcctatc ctgacatggt tttgctggct gatggcgttc ctgtcatcat
     6241 tgaaactact cccgcgcagc gctataaaat caatgcacaa caactggagc aagccatcac
     6301 tccaaaaacg cgaatgattt tcctgaacag cccttccaac ccctctggaa tagcctacac
     6361 ccagaacgag ttaaaggaat taggcgacgt tttgaaaaaa catccacaga tacttattgc
     6421 tactgatgac atgtatgaac atattatctg gagccaaccc tttatgaaca tcctcaatgc
     6481 ctgccctgaa ttatatgaca gaaccatcgt attaaatggc gtgtccaaag cttatgccat
     6541 gacaggctgg cggataggct atgcggcagg tcctgcgcca ttaatcaatg caatgaaaac
     6601 cattcaatcc caatccacat ccaatccatg ctccatagca caaagagctg ccgtagcagc
     6661 tttgaatggc agcaatgaaa gcattgaaga aatggtcaac gccttccatc aaaggcacga
     6721 ctacgtggct gatagattgc aaagcatcga tggcatagag gtcatccccg ccgacggaac
     6781 tttctacatt tttcccagtg tgcaggcgat tatcgaaaaa cgaggttatg ccaatgacat
     6841 cgaattttcc gaaaaattgc tgaatgaagt aggtgtggca ctggttccag gctccgcttt
     6901 cggcaccgaa ggctgcatca gaatatcctt cgccaccggc attgacaccc tgaaagatgc
     6961 attgaacaga ttacaacgtt tctgcagtta aaaaatatat taaaaacctc ttgattctct
     7021 cgcggaatcg ttttaagatt ccgcctcaat tccccggtag ctcagtcggt agagcggatg
     7081 actgttaatc attaggtcac aagttcgagt cttgtccggg gagccaattc cagtctgggt
     7141 tttggccaaa atcaaatttc ttcgtagttt ctctgggtac actttggggt gcactttaat
     7201 ggaaaaggag agctaccaag ttaacggatt tatacatgga tgcatcaaaa ggaaataatc
     7261 accttaaaag cttaaataaa tactcctggt tcattcttgt aatgtttatt ttcgctgtat
     7321 tcgcaatgtc gtaccagaca accaatactt tttttgatgg ttttattcaa acacttcctc
     7381 taattattgt cttcgttttc tggagtgaaa aatctgcacg acttataaaa caagctgaaa
     7441 gtaatttaaa aagggcagaa ctctttaatc gggatacgtt tatattaagc tttagttttt
     7501 tattgggatg ccttatttca ttactatttg cttatgataa tagtgatgtc aaaggttggt
     7561 gggtattaat tatttacttc attaccttgt atggtttaat tttttcttta attttctcag
     7621 gaatagccct tcagataaaa aatcataaaa catacaccct ggttttttca cttttaatca
     7681 tagtattcgt atcaatggga aaatttttcc cccgttatac gttcattcca ttgctaggat
     7741 atattgacac tttctacgca gtaacttgcg tattgttaat tattcattgt ttatttgcct
     7801 ttaattgcaa aataatcaga gcaattaaga gaaacacacc ctaacaaaaa gttcaacaaa
     7861 agatggagct gcaaatgaaa agtaaaaaaa tactcatatt agggttaagc attacactaa
     7921 ccgctataac ttcagcatca tatgccaaga aaatttttcc ttctgatatc ataggtagag
     7981 atttaaatta tcccggcatg ggatggttag gacatgttgg cattgctaca aggccaatga
     8041 tggattcaag tggcatgtca acgcctgcta atcaagtgat agagatactt aatgaaccag
     8101 taataggtca gattaatact atagataatt ttaaaaggcg ttccccctat tggggtagta
     8161 aatatggagt aattggtaac cctgagcagg gctatcgagt attggttgaa gctaatcacc
     8221 agcgttggtg ggttagagaa tataccagca atactgatta taggattgcg caagggaatc
     8281 caaggacagg agaaatattt gtaacaggtc gttggcgatg tgacacttat gcttggtggg
     8341 ctttttacag ccaaggagta gacaccatgc ctggtcgcat atggctacca cggaatttgt
     8401 ttaatttttt tccttattat aatgatgaga ggttggttgc agaatcctcc gaaccatcag
     8461 cagaaatttt aacaaataaa tccttagacg atgtaagcgc tgaagatctt aatgaaatgt
     8521 ccttagaaga gtttcagaca gtaatcgacg cccccttaaa ggctccaact aattatgttg
     8581 ctcctatgtc agcttatatg cgttttgctt atagcgatag tcttaatgat attaaacgag
     8641 gggctatgat tgataaaatt acatcaaagg ttagtgacgt agatttagtt ccaaaattat
     8701 taagattata tgatgaaaca gaccgagagg aggtaaaaaa taaaatagta agcggcctta
     8761 tgtttcatgt tcaaggagag ttaagagaac acccaaactc cagagataaa gaattattga
     8821 gaagtttttt tcataggcta ataggcgaaa agctcaatca acgatgcgcc gattgggcca
     8881 caatgggatt tattgacaca catacggcag atgaaaccct acaaaatctg gataagatag
     8941 atgctcaatt agcaactgta acccatccgg cctcaattac gcttaaatac aaattagtcc
     9001 ataagtctaa aaaattacaa tccatttata tcaagtcgat tgtggatgag cttcgcgagg
     9061 ctaataattc agacttggac agctattttt ttggccccct agctattggg tatcaaggaa
     9121 ctggaaaaaa tttgctggag ccagaatcca aacaaatagt tattaactat ttagatgaag
     9181 ttcgttacaa atatacttca aaaggtatca aagctaatcc aggtgacttt catcgaggca
     9241 ctacagcgcc ttattatttt gaattactca aacatatgga taattaaact gttctctgcg
     9301 ccagatcact ttctggcgct atcttttaat ttttgaagta tctcaattta atcagttctt
     9361 tttatcaact aaattagctc acgcataaaa taatgcgctt atccataact accgttaaat
     9421 tatgcagctt tacagttact gccctgtttt gagtttagtg ttgatcgagc tgttcactca
     9481 aagttaaaac ccggcaatgc catattgctc gaaatagggg gcaaatcaac cgttataagg
     9541 caaacggtct cgccgattta caactgtttt ttagtgctta ttttctcctt attttgatga
     9601 ttaataaaaa cgacaagaca atggctcttg tcatacaagt tacaagtcca tgatcaaatt
     9661 aaataaactc ccaggggagt tgccctatcg gatgacagag attcctagat ggttttccct
     9721 actgaacgtc ccgccacggg gttatggaat gaagtggctt agcataagac atcagttgat
     9781 gtttcgccag ggtttcacta gcgcatcagt tatgcttgat tccaaattag caaagcggtg
     9841 cttgcttagc aaggggtttt ggaaaggttt ttgtccaatc ccgattggcg ggatcggaca
     9901 aaatgctaaa atgtgatgat tttactcggt ataattaagg tgatgtatca ttgcgacttc
     9961 agtcacttga gacttattct gttaattctc catgtcatat acaatatgct caaacacata
    10021 cggatctgtt tgccaacatt ttttggccaa ccaattagct ggatatttcc attttggtac
    10081 ccaactaccc agcattacct gaattttata actttcaatt tgcccatgta gtcccgtcat
    10141 tatcttttca aataacgtat catcaggatt aagttctcta aattgataaa aagccgcttc
    10201 cctgtcaacg ggatgtggat aacttttcca aaatttttca aatttgctac acatataaat
    10261 ataattataa tcttctgact tctgaggtgg gtcggcctca gtgtaattcg ctatgttggg
    10321 tttttgtcta aaattacatg attcccccga ttctaaagta tcttcatgtg tcgggataaa
    10381 ggttcggctt cggtacggcc ttctttggtg cagggttatc caattcagca taaatacact
    10441 tcaatatcaa gtgcctttca gttgactgaa tctcaataag tccggcacgc tctaatgatt
    10501 taacagtccg cttcatttgc tgtaggcttg gagtccctgt ttttactcca gcaatcggcc
    10561 caacatagag agtttcacgt aacgattgat aactgatcct gcgcttgata cctacaatgc
    10621 aactttgtct gtccatgtat gggcgaatac ccatcaaata aacgactctt tgtatgtagg
    10681 ttccagtaat gccgctaatt catgagcatt ggcgaataaa aaactcaagc taaccatcct
    10741 tttggagctg tttgttattg aataatatta actgttactg aaagtcttta aaaaatggat
    10801 gtgcctcatg catcccaagg ttgaaaaata agatggggac gcgtctaact cagtagttac
    10861 gcgggatcca cgtgtcccgt gcctctttta accattggca ttagggctac aatgtgttcc
    10921 cttggtaatt gtgacacaac ttgtgtcgta attgatgcat ggccagttgc tttatcagtg
    10981 taaattctgc atattcaatc attttttacc ctcaaatgcg ggtacaattg attttacccg
    11041 cacttgaggg tatagttaat attattggcg attgggggta aaaagaggaa atcgatgatc
    11101 attagctcag caaaagattt agcattatat ttaaatgaca agcgcaaaca actcaaaatg
    11161 agtcaatctg aagttgccga tttagtcggc ttaaaacagg ataccatatc aaagtttgaa
    11221 aatagccccg ataatagtcg aattgatacc ttatttagaa tattgtccgc cctaaattta
    11281 aatatgagcc ttgttgaaaa agatcaaaaa actcatgaag agatagaatg gtaatgcgta
    11341 aattatacat attgatgaac aatgagctcg tgggggagct tacccaggat aatgcaggtg
    11401 gtttgagttt ttcctacgtt gctgactgga tttccaaacc gcattcacgc ccagtttctt
    11461 tatcgttacc gttagtaaat caaaaatttt caggagaagt tgtttataac ttttttgata
    11521 atttacttcc cgataatcca cagataagag ccagaattca agctcgtttt caggcaaaat
    11581 catcgcgacc gtttgattta ttggcgttaa ttggcagtga ttgtgttggt gcaatacaaa
    11641 ttgttgaacg tattcctcag acacagcgtc taagcattga aagtcacgtt gtcagtgagg
    11701 ccgagatagc tcgtacttta cgttcttacc aaacctcacc actgggaatg gataaagagg
    11761 atgatgagtt tagaatctca atagccggtg ctcaggaaaa aacggctttt ctatattacc
    11821 aaaatgaatg gcataaacca tttggaccta caccaacaac tcacatattc aaattaccta
    11881 ttggatattt accacatcaa ggaattgatt taagtgagag ttgtgaaaat gagcatctct
    11941 gtttactact agccagcaaa tttggtttag atgttgcaaa atcaaaaatc ttacaattcg
    12001 aagatgaaaa agtgctggtt gtcgaaagat ttgatagaaa atggagtcga gataaccgtt
    12061 atatactcag gttaccgcaa gaagacctat gccaagcgtt gggttattca ccaaatttaa
    12121 aatatcagtc agatggtggc cccggtatta aagaaattat gcagctactt aatggctcac
    12181 agcagcctca aaaagacaga gatgaatttt tcagagcaca agttgtttac tggtgccttg
    12241 gtgctattga tgcgcatgcc aaaaatttca gtataagtat acaaccacaa ggcggttacc
    12301 agctcacccc cttatacgac atcatttcag cctatcctat cgtggaacaa aaacagctat
    12361 cctttaagaa attgaaaatg gcaatggctt tatatggtaa aaatattcat tacaagattg
    12421 acggtattca aaaaagacac tttattgaaa cagcaaaata cgttaaatat tctaaaatta
    12481 aagcagctca gatttttgaa caagtgattg accgtattga ttctgctatc gatgaggtct
    12541 caagacaatt accaaaccac ttcccagaaa agattgcaga tccaatcttc aaagggatga
    12601 gagcttttaa ggagcgaatg atatccacat agtaactaaa tgtcccttaa tcaaataaat
    12661 cgttatgaat tttgattcca tttttcacat tttactccct gttacggttg gatttctaac
    12721 agggttgccg aaggttgaat acccatcttc ataaaataat cagtaatttg ttacatgggt
    12781 tttcttaaac gctttaaagc ataagccgag atatccagcc cctcggcaat agtgataaga
    12841 gttcgtctta aatcatgaac cgtaaaatga atacctgaga gtttggtcac attggccatt
    12901 tgcttacgtg gctcaatgat atgacctgct gctcttgtac ctggaaaaac atattcataa
    12961 atcctctttt gattgcggag tcaaagagtt agattgctat aacccgatta tagtgccact
    13021 acctttggag tagaaccgac ttgcagctta gctcgtccgt ctcttcatcg tttggcctga
    13081 gagcggtttt attcacaggc tggaagagtt tagtataatg ttctttatgt tcttggtctt
    13141 tcgagagcgc agactcttgt gaaagctcat gcgtacaaac tttctcttgt gttttttcca
    13201 cagtgcccat ctcttcgata tcatacgggt tgtccagatt cgcatcaata agtgatatgt
    13261 atctatcatt tgagagaact tcttttaaaa gcgcattgtt tttattaact tttctataaa
    13321 actgcatcgc cgactttgat aggcttacag agaatacagc caaagatata ccgagcaata
    13381 tggggccaat tggccaggca gcgattcccc aaaacagacc tgacactcca acaataaggc
    13441 ttgtaagaga agtgccaagg tcaataaatc tctccagact gcgggtgtgc ttgcgatgac
    13501 atacgatgtc gtaaagtcta gctagcccct cttgtttagc aacggctaat ttcttgtcaa
    13561 gttggtaaag cttaaaaact aacagcactc gtgcttgctt atcgtcgagc cgcgtgttga
    13621 gaagtttttc gtacttggct tgtcgctttt ctttaatact ttccacatat tgttcagggt
    13681 tatctttata ctgatttatg agtgcatttt ccttacgcac gtccgaataa aggtagtatg
    13741 acgaattaag tacgccaaac cccataatgc ctatgccaag atatacccac atcgcaggga
    13801 ataacgttcc cagatagatg agtggtatcg cccctgctgc agctgcaatg agaataccag
    13861 ccgacatcac acgacttaat ttagaggctg ttggatcaag tattcttgca atggtactgc
    13921 ttacaaaacc aatgactgaa aagagcactc ccagaatgcc cagggcaatg cccgcgcttt
    13981 ccgtaagtac aatgatgcgt gtcatgtact gcagaatgct cgacagagag tttgtggcga
    14041 gccccgtact gcgcaagtag acaaaggctg aattattgtt ctgtaggttt ttcttgattt
    14101 ctttgttcat cggcagaact ggaataaata taaaaaatgt gcattatata aggtttgtct
    14161 taagagaact ttaacaaccc tggatatcga tagctcgatg atcaactttt tatatattga
    14221 cagtgtatag ctagcgtagc actgatgcaa gcgccgtcat actcccggat ttcgttccac
    14281 ttcatccggg ctacgcttgc taattgaatg acataagcaa catgaataaa gccattccta
    14341 tcatcattaa attttcagtg agagaaataa atcctaaggg tacgttacta tcaccgccca
    14401 cacaggcaca tttaagctcg cgcttgtcga tataaacagc tttaaaaaca gaaagagcac
    14461 ctacagttcc aataaataag gcaattggaa tggaaagcca atttaacgca tctgcaatca
    14521 ttaatatccc tgctaaagct tcagcaaatg ggtaaatata gctgtaagga acccagcgtt
    14581 tggccaagag atcataattt aaaaacatgg ttgagaagct ttcgatattc tggagcttta
    14641 ataaagcaag aactgccatg ctaaatgcga taaaccagct taaaacatgt ggtgtaaaag
    14701 ggttagcaaa gctagcataa ctcactgcca tcgccattaa agcggtaatc gtaaacagca
    14761 caatgacggg gcgataactt ttttcttttg gatctcttac tttctttcca aagtaacgcc
    14821 gtaaatcgtc ataaccccct atacgctcat cgttgataaa aatttgaggt gtggttttta
    14881 cattgtgttt atccttaaac gcatcgacct gatctcgggt tgttagccaa ttatcttcta
    14941 cctcatagcc ttgacgcctt aacaaatctc tggctttgag accataaggg caaacatggt
    15001 gctctatcac catgcgatac aaaactgctt ttttaattga ggtatggttc atttctatcc
    15061 cctatgatta atggcctata aatagtaata tagagtgcgt actatagtac ggagtcaagt
    15121 aatgaataat aaaaaaattg gtgaatttgc taaattatgt ggagttggca ttgaaaccgt
    15181 gcgttattat ca
//
LOCUS       DASLOE010000065        14722 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.065, whole genome shotgun sequence.
ACCESSION   DASLOE010000065 DASLOE010000000
VERSION     DASLOE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14722
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.065"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            20..1153
                     /locus_tag="U6309_001571"
     CDS             20..1153
                     /locus_tag="U6309_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated sensor domain-containing
                     protein"
                     /protein_id="HEN5505013.1"
                     /translation="MTSSKHYKESMTLLFEGSLRAIPFNIILATLLALDLVYNDVPAL
                     IIGAWFFGIVFISLVRSYFCWKIIKRGVEKNRIQATLIEFFLLTFVTGSIWGACYFIT
                     LPYLNDLHEFIIILVFGGMCAGAIASLSIYLPAYYAYVFPMLLPVIVYNYALLDLDRT
                     MLATMFLIFIAMLIISAKINHRLLNENFRLFNEKELLINELKVMSITDSLSGLYNRRY
                     FDNIFPQEFNRARRNQYFLSLVSIDIDNFKLINDNLGHPFGDKVLISIADLLKKIFRR
                     SNDILFRLGGDEFAIIIANQPIKETISICKAIKNPFKTENLNHTYDSNEQLLMSHVTL
                     SIGIAYIHFESSVSIEHAITAADKALYQAKKKGKNQIVVKKLL"
     gene            complement(1216..1857)
                     /locus_tag="U6309_001572"
     CDS             complement(1216..1857)
                     /locus_tag="U6309_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505014.1"
                     /translation="MKMKLQLIAIGILLSINVFADGYYPEPTSQDQPWTIIASFGSGR
                     YQHMYSKDGRTALGRLALANELMLSGDIALGLELGVQNGNKMRLNIPRETLSVLGWLP
                     VETNLSPMLDLLVTAKSDPLFGSSFFAQLKGGVAYRSWQVDLYPINEISQLAGEIQAG
                     FGYPITALASLSLLYQGVFGNDPNVKLNVSSKTGSVSNIPVLHGILLGFTVNL"
     gene            complement(2030..2117)
                     /locus_tag="U6309_001573"
     tRNA            complement(2030..2117)
                     /locus_tag="U6309_001573"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2081..2083),aa:Ser,seq:gga)
     gene            2340..2774
                     /locus_tag="U6309_001574"
     CDS             2340..2774
                     /locus_tag="U6309_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505015.1"
                     /translation="MTLQHSIQKGVSDFSFIIGTGLKYGGAGGLLVMAVVGTVAIDLV
                     LLAAAEKHHNSFLTGFVLGSMFSRGNLDPVPLLIASPITSAVAVVLSVALGVPGVGLA
                     ILAGWTLAGTLLAVGMGLEALAKSIDPEPSSESDYNSCLCFN"
     gene            2896..3342
                     /locus_tag="U6309_001575"
     CDS             2896..3342
                     /locus_tag="U6309_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505016.1"
                     /translation="MYLRLLALSALCFVTSPIWSFTCIYTLVKDNCWTDYDVTVDVIE
                     DSTSKTLLTLTAPKGKSWARGTFNCEAAEGLRYVAQFSPVFWQNDVGKTYPALRNWYL
                     PAKVNPGDLAWTIPVCFPADFAQVPFPPNVAGNCKCNFKNIPDPKL"
     gene            3470..4546
                     /locus_tag="U6309_001576"
     CDS             3470..4546
                     /locus_tag="U6309_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEN5505017.1"
                     /translation="MITIRNKIGQMLIMGFHGSELHDNSPVAQWLSNDGLGGVLLFDK
                     DLSTGIYGKNLRNQAQIRQLIRQLNRYASMISGGNDNLPLFTAIDYEGGVVDRLSKIE
                     GCPPTMKARELAKLSSDDFDEEVTQMAMTLQSLGFNLNFAPVVDLNLNAHAGIIGNLG
                     RSFSSNPEVTIRLAKQFVDVFSRYGIACAYKHFPGHGSATGDTHEGFVDVTETFQTDE
                     LIPYRSLLKDLSNPVMVMTAHVINKHLDSKGLPATLSYDVLTLLLRQSIGYDGVIISD
                     DLQMHAISNHYSLEEALCLTINAGADMVIFANQLGTITAPEVIDVIEKLVIDKQISSQ
                     RIDEAYRRIVRLKQQIICSELINT"
     gene            4933..6213
                     /locus_tag="U6309_001577"
     CDS             4933..6213
                     /locus_tag="U6309_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEN5505018.1"
                     /translation="MCAAHHQKNKYIFSTPLIYAAMIGLGIISGLSDLPFLKETGLLI
                     SDLFIKLFKCISLPIISLSIIVTLANYQTDSLMKSVWQRTIKYTFATTLVAALISCFL
                     YILIHPSTVQVNLDAANPKSTSSLGYLGYLSNMIPTNLLSPFLEQQVMGVLLLSIVIG
                     FAVRQIPETEPRETIIRFFRGAHGMFLVMTKWIIKLIPLGLFGFITSTVIQLRSGMDI
                     KGIGQYLMIIVLANLIQGLVILPLWLKSNGIKPFSSMRAMLPALSVAFFSKSSVGTLP
                     VTMNTVEANLKVNPQVSRFVLPLCTSINMNGCAAFIFATVIYLMQSHGIQVSYATMGI
                     WVLVSTIAAIGNAGVPMGCFFLSVSLLSSMNVPITLMGVILPFYGLIDMLETALNVWS
                     DACVTKVVNDKAEGGDGVVGIRPKERSLLNAELG"
     gene            complement(6536..7669)
                     /gene="dapE"
                     /locus_tag="U6309_001578"
     CDS             complement(6536..7669)
                     /gene="dapE"
                     /locus_tag="U6309_001578"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEN5505019.1"
                     /translation="MTDITQILTDLIGFPSITPEDAGCQKYMIQFLEQLGFTCQQLNN
                     GPVSNFFACYGKIGPLLVFAGHTDVVPVGEVSKWDTDPFSLEEKNGMLYGRGVADMKG
                     SLACMLHMARRFIKTYPSFPGRLGFLITSGEEGDEFNLGTPYVMQKLEQQGIVIDYCI
                     VGEPSSSLKTGDVIKIGRRGSLSAKIHLSGKQGHVAYPHLADNPIHRISPVLAELTSM
                     QWDNGNAYFPPTSMQITYIHCGGHAGNIIPGELNLHLNFRYSTEQTDESLKTRVINAF
                     THHKLNPTIEWRFNGEPFLTNKGILLESCKQTVLEHIGTLPELSTSGGTSDGRFIAPY
                     GVEVIELGLVNATIHQVNECTSLQDLNTLETMYFSICEKLFID"
     gene            complement(7662..8492)
                     /gene="dapD"
                     /locus_tag="U6309_001579"
     CDS             complement(7662..8492)
                     /gene="dapD"
                     /locus_tag="U6309_001579"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEN5505020.1"
                     /translation="MNSLQALIEQAFENRQNLSLNTVSSDLINAINEVLSGLDNGQFR
                     VAEKINGEWTVHQWLKKAVLLSFKLFPNQIIDAGFCKFYDKIPLKYTDSSNEQFQQSG
                     VRVVPHAMVRRGAYIAKNTVLMPSYVNIGAYIDEGVMVDTWATVGSCAQIGKNVHISG
                     GAGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVIVEKNSVISMGVFLGQSTKIYNRI
                     TGEVSYGRIPAGSVVVAGNLPSHDGSHSLYCAVIVKQVDEKTRAKVSINDLLRANQDD
                     "
     gene            complement(8496..9389)
                     /locus_tag="U6309_001580"
     CDS             complement(8496..9389)
                     /locus_tag="U6309_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN5505021.1"
                     /translation="MQKNKQNGGQLHAIAAIFALIVSTTLCFIPILFVGLLKLFPNKN
                     WQSLCTRCVDRIATFWCGINNFYVAKAQKIHWEISGLNNLTPKDWYLVVANHQSWLDI
                     VILQRLFNRKIPVLKFFIKDQLKWIPLLGFSWWAMGCPFMKRYSKEYLAKNPHKQGKD
                     IISTRKAIETFKRTPASIMNFVEGTRFTKVKKEQQQSPYNYLLKPKAGGISFIINSMG
                     QQIKSLIDVTIVYAEKNHSLWDFLCKRVKKVKIIIRQLPIPAQFTSANLINDFQAQNA
                     FKEWLNEQWAIKDNLIASLQE"
     gene            complement(9456..10607)
                     /locus_tag="U6309_001581"
     CDS             complement(9456..10607)
                     /locus_tag="U6309_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEN5505022.1"
                     /translation="MNKTHFDTRAIHAGQEPCQSTGAVMTPIYATSTYKQIAPGEHLG
                     YEYSRTQNPTRKAYEDCIASLESGQKGFAFASGMAAINTVIDLLDSGDHVLAMDDLYG
                     GTFRLFDKVKTRTSNLSFSFIDMSVPENIEAAITPKTKLLWLETPSNPMLKLANLRKI
                     AAIAKKHNLITVADNTFATPWIQRPLELGFDIVLHSATKYLNGHSDVISGVVVVGDNP
                     MLSDKIAFLQNSCGAVAGPFDSFLVLRSLKTLSLRMQRHCENANHLANWLNGHPKIEK
                     VIYPGLKSHPQYSLAKEQMNDFGGMISLVLKGGLEDAKRFLARCELFTLAESLGGVES
                     LIEHPAIMTHASIPVEQRKALGIEDGFIRLSVGIEHIDDLRADLEHALG"
     gene            10756..13071
                     /locus_tag="U6309_001582"
     CDS             10756..13071
                     /locus_tag="U6309_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEN5505023.1"
                     /translation="MRRQEINYEKVAKAAEKIKKRGIEPSVNEIRDELGLVGNHPQLS
                     ILLEEWYHNQPEFKRKSHTPLTENINLNTDEIREKNVELEKSISLLRATLESTADGIM
                     MVNGHGAVVDWNQKFVEMWRIPSYMMESGKESISFEYILEQLIDPQSLIADVQYLYQN
                     LEWQGELPELHFKDGRIYERFTQPQRVGSQIVGRVYSFRDVTQKRMALDELRIRERAI
                     EASTHGVVIIDVTKNENKVIYVNRAFERITGYGEQHALGKGLLTLLGSNLEEVNHKRI
                     ELAIRESKEETIEMESIKRNGEFYWCEISVAPVKDSFGYVKHYICILNDVTQRRDMED
                     QLLQQATYDSLTNLPNRVLLMDRVEQAILQARKNKAILAFLFLDLDRFKLTNDTLGHS
                     MGDKLLQAIANRLLIVTEDFDTVARLGGDEFVILLTDIDNMLEAETIAQNILKIIEKP
                     IQIDQHSLKITGSLGISFYPRDGDDYESLMKSADLSMYHAKDTGRNNYRVYEPEMNRR
                     VINHMQLDNALRDALKNDELFLVYQPLIDLKQSRVVGFEALMRWHSKILGLVSPADFI
                     PMAEENGMILEMGEWAMKQACMQVKEWHKAGFKNLSIAVNLSGRQFRQKNLPEVVSRV
                     LKSSGLQSRFLELELTESLLIEDIDHVVDTMYALKDMGTKLVIDDFGTGYSSLSYLKQ
                     FPVDKLKIDRSFITEMVSNQNDAAIAKAIINLGHSLNLQVLAEGVENEFQRDFITSHG
                     CDYAQGYFFKAPDTPENILEFLKSLSESKIK"
     gene            13373..14722
                     /locus_tag="U6309_001583"
     CDS             13373..14722
                     /locus_tag="U6309_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEN5505024.1"
                     /translation="MKHITIIGAGLAGTLCGLYLARRGYEVELFESRPDIRNSPTDYG
                     RSINLALSCRGITALKAMNLLSEVNKIMVPMRARAIHEANGEVHYQPFGRHIDEYINA
                     ISRSDLNALLLNKAELCPNIKLHFNMKLHSLDIHNKKIKFENKNGDFVEVSYHRLIGA
                     DGAPSHVRDMLKNEGIVSASRDFLSHGYKELSISKKHTKGMAREHLHLWPRDSFMLLG
                     NPNPDDSITGSLFLANEGKDSFAELNNEEKLHLFFKTQFPDAYAAMPNLVQEFFGNPT
                     GHLSTIQCSPWYYKDECLLIGDAAHGIIPFFGQGMNSAFEDCRILDELLDEYQDDWSR
                     VNPVFYEHRKVNTDAIAKMSMDNYHEIHSDIRNPKFILQKQIERELMLRYPEHYVSMH
                     VLVMFTNTPYAKAMAIGELQSGLLEQICFPITDIKELNWQEVEKLLSVYDKKLAKII"
BASE COUNT         4469 a         2546 c         2975 g         4732 t
ORIGIN      
        1 ttaatggaca ttggataaga tgacttcctc caagcattac aaggaaagta tgaccttgct
       61 atttgaggga tctctaagag cgattccgtt caatattata ttggctacat tgcttgccct
      121 tgaccttgtt tataatgatg tgcctgcatt aataataggg gcatggtttt tcggtattgt
      181 ctttataagc ttggtaagaa gttatttctg ctggaaaata attaagcggg gagtagaaaa
      241 aaatagaatt caagccactt tgatcgagtt tttccttctt acatttgtta cgggcagtat
      301 atggggcgcg tgttacttca ttacactgcc ttatttaaat gatctgcatg aatttattat
      361 tattttggtg tttggtggaa tgtgcgcagg cgcgatcgct tctctgtcta tttatttacc
      421 agcttattat gcctatgttt tcccaatgct tttaccagta attgtttata attatgcctt
      481 gttagatctc gatagaacga tgttagcaac catgtttcta atatttattg ccatgcttat
      541 tatttctgca aagattaatc atagattgct caatgaaaat tttcgattat tcaatgaaaa
      601 agaactatta atcaatgaat tgaaagtaat gtcaatcact gattctttgt cggggttata
      661 taacaggaga tattttgaca acatatttcc acaagagttt aatcgagcaa gacgcaatca
      721 atatttttta agcctggtat caatagacat agataatttt aaattaataa atgataattt
      781 ggggcatcct tttggcgata aagtcctgat tagcatcgct gatttattaa aaaaaatatt
      841 tcgacgttca aacgatatac tgtttcggct tggcggagac gaatttgcaa taatcatcgc
      901 taatcagccg attaaagaaa caatatctat ttgtaaagca attaaaaatc cttttaaaac
      961 agaaaatttg aaccatactt atgactcaaa cgagcaatta ctcatgagtc atgtcacttt
     1021 aagcataggg attgcttata ttcattttga aagttcagtc agcatagagc atgcaattac
     1081 tgcggcagat aaggcattat atcaagctaa aaaaaaagga aaaaaccaaa ttgtagtaaa
     1141 aaaactactt taagaagatg acattctatc tttaactgag atggcagcct ggaagctgcg
     1201 tcgtacttta atggattata agttgacagt aaatcctaat aaaattccat gtaaaacagg
     1261 gatatttgaa acactgccag tttttgagct cacatttaat ttgacgttag gatcattacc
     1321 aaaaacaccc tgatacagca aactcaaact ggccaaagcg gtgataggat aaccaaagcc
     1381 agcttgaatt tctccggcta attgtgaaat ttcattgata gggtagagat caacttgcca
     1441 agacctgtaa gcgacaccac cttttaattg tgcaaaaaaa gaacttccaa ataaagggtc
     1501 gcttttggcg gtaactaata aatccagcat aggactgagg ttggtttcta ccggtagcca
     1561 tccaaggact gataaggttt ctcgtgggat attcaagcgc attttattgc cattttggac
     1621 cccgagctct aatcccagag caatatctcc actcagcatt agttcattag ccagagccaa
     1681 tcggcctaaa gcggttctgc catcttttga gtacatgtgc tggtatctgc cacttccaaa
     1741 actggcaata attgtccatg gttggtcttg agaggtgggc tcaggatagt aaccatcagc
     1801 aaatacgttg attgataata aaattcctat tgcaattagt tgtaatttca ttttcatctt
     1861 tcatatccct gattgaattg gttggttctg aaagcagttt taccaaaaga atcttatatg
     1921 agccagctgt agtctttaca caatggatat aatttactgc tttatgcaac tcaatgaaag
     1981 cggcaattat agccttatac cctgtgaagg ataaccagga attgtaattt ggcggagaga
     2041 gagggattcg aaccctcgat acgttgccgt atacacactt tccaggcgtg cgccttcgac
     2101 cgctcggcca tctctccaac cgggcaagct tatacttgtc ggcaaataaa agcaagactg
     2161 tgattattat taaagaggtt ttccatcaca aagataatat attctatttt tgtatgggaa
     2221 taaattgtat cgttttattt gagttttaat ccaatctact cgttatttca gtgttcaatt
     2281 agtgttgatt atgtgcaaaa ttaagtgtat tattcctcaa ttttttagga gtaagattaa
     2341 tgacactgca acattccata caaaaagggg tatctgattt tagttttatt attggcacag
     2401 gtctaaagta tggtggtgct ggcggtcttt tagtcatggc tgttgtagga acggttgcta
     2461 ttgatcttgt cttgcttgct gctgcagaaa aacatcataa cagttttttg acaggatttg
     2521 tgttaggttc aatgttttcc agaggtaatt tggatccggt tccacttttg atcgcgtccc
     2581 ctatcacatc agctgttgct gttgtgttat ctgttgcctt gggtgttccc ggggtcggtc
     2641 ttgcgatact ggctggatgg actttggctg gcactttatt ggctgttggt atgggcttgg
     2701 aagcgcttgc taaatccatt gatcctgaac cttcatctga gtcagattac aattcttgtt
     2761 tgtgctttaa ctaatagagc caagagggga gatcagatta tttcccctct taaaacagtt
     2821 tatcggaaaa ttatttttat aaatattgta tatactatat agtaataatg gttcattaaa
     2881 tcttaggatt gaatcatgta cttacgttta ctcgccctat ctgcattgtg cttcgtgact
     2941 agcccaattt ggtctttcac atgtatctat actttggtta aagacaattg ttggactgat
     3001 tatgatgtta ctgtcgatgt cattgaagat tctacgtcaa aaactttgtt gacacttacc
     3061 gctcccaaag gaaaatcatg ggctagaggt actttcaatt gtgaggctgc tgaagggttg
     3121 agatatgtcg ctcaattttc gcctgtcttt tggcaaaatg atgtcggaaa aacttacccg
     3181 gcattaagaa attggtattt accagcaaaa gtgaatcctg gagatttggc ctggactatc
     3241 cccgtttgtt ttccggcaga ttttgctcaa gttccctttc cacctaatgt agcaggaaac
     3301 tgtaaatgca acttcaagaa cattcctgat cccaagcttt aattttatga gaagcatttt
     3361 tttatttgta cacattgcca tactatttat agataatgcg tagcggggca tagccagaga
     3421 gaatatcttt gtgtatgatt ttatgcttat tttgtgatgg agcgagtgat tgattacaat
     3481 aagaaacaaa atcggacaaa tgctcattat gggatttcat ggttccgagc tgcatgataa
     3541 tagtccggtt gcacaatggt tatctaatga tggtctcggc ggagtcttgt tattcgacaa
     3601 agatctatcc acagggattt atggcaagaa tttaaggaat caggctcaga taaggcaatt
     3661 gattcgacag cttaatcgtt atgcatcaat gatttcagga ggtaatgaca atctgccttt
     3721 atttactgcg attgattacg aaggcggagt ggttgacagg ctaagcaaaa tagagggctg
     3781 tccgcctact atgaaggcca gggaacttgc aaagttatcc agtgatgatt ttgatgaaga
     3841 agtaactcaa atggctatga cgttgcagtc attaggtttt aatttaaatt ttgccccggt
     3901 tgttgattta aatttaaatg ctcatgcagg gattattgga aacttgggaa gaagcttttc
     3961 ttctaaccca gaggttacta ttcgtttggc caagcagttc gttgatgttt tcagtcgtta
     4021 tggtattgct tgtgcgtata aacatttccc cggccatggc agcgccactg gagatacaca
     4081 tgagggattt gtagatgtta cagaaacctt tcaaaccgat gagctaattc cttatcgaag
     4141 tttattaaaa gacctgtcta accctgtcat ggtaatgacg gcacatgtga ttaataagca
     4201 tttggatagt aagggattgc cagcaactct gtcctacgac gttttaacgt tattgttacg
     4261 tcaaagcata ggatatgacg gggtcattat cagcgatgat ttgcaaatgc atgccatttc
     4321 aaatcactat tcattagaag aggctctttg tcttaccatc aatgccgggg ctgatatggt
     4381 catatttgcc aatcagcttg gaactattac cgcgccggaa gttatagatg tgattgaaaa
     4441 actggtaatt gacaagcaaa tttcctctca acgtattgat gaggcataca gaagaatagt
     4501 acgtctgaag caacaaatta tatgcagtga attgatcaat acgtaatgcc catgccaagt
     4561 ggtagctggc ggatttatga accatcaagt taccaccata acttcaccta ttagagaggc
     4621 tccatcttct tttcagcatg aacagacata attgtttgag attgggtttg aagtatttta
     4681 ttagtatcta tatggtgtgt tctgtgaaga atataattga cacagtttat ttatcctatt
     4741 gactagaaga aaaatcgtgt tacaatatgt actctaaatg aacaatttta gctatgggtg
     4801 atgactttta tgtcaattca atcattatat tattataatt cctcactatt tcactccata
     4861 tatcaagaca tctgttgttg ctgctaatcc tactttgctt aaataattaa ttaatgtttt
     4921 aaggattccg ttatgtgtgc agcacaccac caaaagaata aatatatttt tagtacccca
     4981 ctaatttatg cagcgatgat tggacttggg ataataagtg gattatccga tttgcctttt
     5041 ttaaaggaaa cagggctttt aatttctgat ttgtttatta aattgtttaa gtgcattagt
     5101 ttaccaatta tttccttgtc gattattgta actctggcaa actatcaaac ggatagtttg
     5161 atgaaaagtg tatggcaaag aacaattaaa tatacttttg ctacaacttt agttgcagca
     5221 ttaatcagct gttttttata tattttgatt catccaagca ctgttcaagt caatttggat
     5281 gcggcgaacc caaaatctac cagcagttta ggatatcttg gctatttgag taacatgata
     5341 ccgactaatt tattatctcc attcttggag caacaggtta tgggggtatt gctgcttagc
     5401 attgttattg gttttgctgt tcgtcaaata ccggagacag agccacggga aactattatt
     5461 cgatttttcc gcggtgctca tgggatgttt ttagtgatga ccaaatggat aattaaatta
     5521 atacctttgg ggttattcgg gttcattact tctactgtaa ttcagttgcg atcaggtatg
     5581 gatattaaag ggataggcca atacttgatg attattgttt tagctaattt gatacaagga
     5641 ttagtcattc tgcctttatg gctgaaatca aatggtatta aaccattctc ttcgatgcgg
     5701 gcaatgttac ctgcgttatc tgttgctttt ttttccaagt catctgtcgg tactttgcct
     5761 gttacaatga atactgtaga ggctaattta aaggttaacc ctcaagtcag tcgttttgtt
     5821 ttacctttat gcacaagcat taatatgaat ggatgtgctg catttatttt tgccacagtc
     5881 atttatctta tgcaaagtca tggcatccag gtttcttatg ctactatggg gatatgggtt
     5941 ttagtatcaa caattgcggc aatagggaat gccggcgttc ctatgggctg ctttttctta
     6001 agcgttagtt tgctctccag tatgaatgtg cctatcacgc ttatgggagt tattcttcct
     6061 ttctatggtt tgattgatat gttggaaact gccttaaacg tatggtctga tgcttgtgtt
     6121 accaaggttg ttaatgataa agcagaaggt ggtgatggag tggtgggcat tagaccaaaa
     6181 gagcgttcat tattaaatgc cgagttaggt taaagccagc aaatcttcaa gcgcaaatga
     6241 gtgtagctta catataagtc tccagaaagt ttttttgaga cttatatcta tgaaatcagt
     6301 ttgtaattta taagagatca gctcactacc tggaacggcc gtatttggtt catcgcgaac
     6361 gaaaataatc cttcaaaaat gctcacagaa caatctatac tgtgcttttt caaggattat
     6421 ttttatccaa actaaatgca aatctgaccg ccagaggata tttaaagatg aggttactaa
     6481 cctattactg taatttggac tgttggtgcg aaccctcatt acagaattgg tttgattaat
     6541 caatgaataa tttctcacag attgaaaaat acatggtttc aagtgtattc aaatcctgta
     6601 gagaagtgca ttcattaacc tgatgaatgg ttgcatttac cagtcccagc tctatgacct
     6661 caaccccata aggtgctata aaacgcccat ctgaagttcc accacttgtt gaaagttcgg
     6721 ggagagtccc aatatgttca agcactgttt gtttgcagct ttctagcaat atgcctttat
     6781 tcgttaagaa tggttcgcca ttgaatcgcc attctatagt ggggttaagt ttatggtgtg
     6841 taaaggcatt aattactctc gtttttaatg actcgtctgt ttgctctgtc gaatatctaa
     6901 agttcaaatg cagatttaat tcgcctggga taatattacc cgcatgccct ccacaatgaa
     6961 tataagttat ttgcatggag gtaggaggaa aataagcatt gccattatcc cattgcattg
     7021 aagtgagttc ggctaatact gggctaattc tatgaattgg attgtcagcc aaatgcggat
     7081 aggcaacatg accttgcttt ccactcagat gtatttttgc acttaatgat ccacgtcttc
     7141 caattttgat gacatcacca gttttcaagc tgcttgaagg ttcacctact atgcaataat
     7201 caataacaat cccttgctgc tccagttttt gcatcacata aggggtgccc aaattaaatt
     7261 catcgccttc ttcgccgctg gtgatcagaa atcctagtct gcctggaaag gaaggataag
     7321 ttttaataaa tcttctggcc atatgcaaca tacaggccag gctgcctttc atatcagcaa
     7381 ccccacgacc atagagcatg ccattttttt cttcgagaga gaaagggtct gtatcccatt
     7441 ttgatacttc accaacagga actacatcgg tatggcctgc gaacaccaat aaaggcccaa
     7501 ttttgccata gcaggcaaaa aaattggata cgggcccatt attcagttgt tgacaagtaa
     7561 atcccaattg ctccagaaat tgaatcatgt atttttggca gccagcatct tctggtgtaa
     7621 tagaaggaaa gccaatgagg tcagtcagaa tttgcgtaat atcagtcatc ttgattggct
     7681 cttagtaaat cattaatgct taccttcgcg cgagtttttt catccacttg tttaacaatg
     7741 acagcacaat ataaactgtg gcttccatcg tgacttggca aattacctgc aacaactact
     7801 gagccagccg gaattctgcc gtagcttact tcgccagtga ttcgattgta gattttagtg
     7861 ctttggccta aaaatactcc cattgatatg acggagtttt tttctacgat aaccccttcc
     7921 actatttcag agcgagcacc aataaagcaa ttgtcttcaa tgatggtcgg gttcgcttgt
     7981 aatggttcta aaacacctcc aatacccgcg ccgccagata tatgtacatt ttttcctatt
     8041 tgggcgcaag aaccaacagt tgcccaggta tcaaccatta ctccctcatc aatataagca
     8101 cctatattga cataagaagg cattaaaaca gtatttttgg caatatatgc tccacgtcgc
     8161 accatggcat gaggaacaac tcttacccct gattgttgaa attgctcatt actagaatcc
     8221 gtatatttta gcggtatttt atcgtaaaac ttgcaaaaac ccgcgtctat gatttgattt
     8281 gggaaaagtt taaaggataa gagaactgcc tttttaagcc attgatgaac tgtccattca
     8341 ccatttattt tttcagcgac tctgaattgg ccattatcaa gaccactgag aacttcatta
     8401 atggcattga tcaaatctga agaaactgtg ttgagcgata aattttgacg gttttcaaag
     8461 gcttgttcaa ttagagcttg aagtgagttc atgttttatt cctgtaaaga ggctattaaa
     8521 ttatctttta ttgcccattg ttcatttaac cattctttga atgcattttg tgcctgaaaa
     8581 tcgttgataa gatttgccga tgtaaattgt gcagggatag ggagttgacg aataatgatt
     8641 ttaactttct tgactcgttt acaaagaaaa tcccagagag aatggttttt ttctgcataa
     8701 acgatagtga cgtcaatcag gctttttatc tgttgtccca tcgagttaat aataaaacta
     8761 ataccgcctg ctttgggttt caataaataa ttataagggg attgttgctg ttcttttttt
     8821 actttagtaa aacgggttcc ttctacaaaa ttcataatag aagccggggt tcttttaaaa
     8881 gtttctatgg ctttgcgagt tgaaattata tcttttcctt gtttatgagg gtttttagcc
     8941 aaatactcct tactataccg cttcataaag gggcaaccca tagcccacca ggaaaagcca
     9001 agtagaggaa tccactttaa ctgatctttg ataaaaaatt ttaaaacggg tatttttctg
     9061 ttaaataaac gttgaagaat gacaatatcc agccaacttt gatgattggc aacgactaaa
     9121 taccaatctt taggagttaa attatttaac ccggagattt cccaatgtat tttttgtgct
     9181 ttagccacat aaaagttatt tattccacac caaaaagtag caatcctatc aacacaacga
     9241 gtgcaaaggg attgccagtt tttattagga aataatttta ataatccaac aaataatatg
     9301 gggataaaac aaagagtagt ggacactatc aatgcgaaaa tagccgcaat cgcatggagc
     9361 tgcccgccat tttgtttatt tttttgcatg aaatacttct cctatacaca tctgtcctgg
     9421 ttctttttaa cagtcagaac caggatttac atcttttaac ctaaagcatg ctccagatcg
     9481 gcacgcagat catcaatgtg ttcaatacca actgataatc ttatgaaacc atcttctata
     9541 cccagggctt ttcgttgttc gactggaata gaggcatggg tcataattgc agggtgttca
     9601 attaaacttt ctacaccccc caggctttca gccaaagtaa ataattcgca gcgagctaaa
     9661 aatcgtttgg catcttcaag accaccttta agtaccaaag agatcatacc accgaaatcg
     9721 ttcatttgtt ccttggcaag agaatattgc ggatgacttt ttaatccggg ataaatgact
     9781 ttttcaatct tggggtggcc gttaagccag ttagccaggt ggttagcatt ttcacagtgc
     9841 ctttgcatac gaagagataa tgtttttaag cttctcagaa ccaggaaact gtcgaaaggg
     9901 ccagctacag cgccgcatga attttgcaaa aaagcaattt tatcagacaa catgggattg
     9961 tcaccgacca caacgacacc actgattaca tcagaatgtc cgttcagata tttggttgct
    10021 gaatgaagta caatgtcaaa tccgagctca agagggcgtt gaatccaggg ggttgcaaag
    10081 gtgttatcgg caactgtaat caaattatgt ttttttgcaa tagcagcgat ttttcgtaaa
    10141 ttagctagct ttagcatggg attggaaggg gtttccaacc atagtaattt tgtcttcgga
    10201 gttattgcgg cttcaatatt ctcgggtact gacatatcaa taaaagaaaa ggataaatta
    10261 gaagttcggg tttttacttt atcaaaaagc cggaaagttc ctccataaag atcatccatg
    10321 gcaagaacgt gatctccgga atctaataaa tcaataactg tattgattgc agccatgccc
    10381 gaagcaaaag caaatccttt ttggcctgat tcaaggcttg ctatgcaatc ctcataggct
    10441 tttctggtgg ggttctgggt gcgtgagtat tcatagccaa gatgttcgcc aggggcaatt
    10501 tgcttatagg tggaggtagc ataaataggg gtcatcacgg cccctgtact ttgacatggt
    10561 tcttgtcctg catgaattgc acgtgtatcg aaatgagtct tattcatttt ttattcccat
    10621 aaaaatattt aaattcaaag aaataccaat aggcgatcaa aataatgatt aaaaattata
    10681 cttttggcgc cttttttgct aaagttcata gtgtaaatgc cgatattttt atagagtcat
    10741 tctagagagg gatgcatgcg taggcaagaa atcaattatg aaaaagttgc caaggccgca
    10801 gaaaaaataa aaaaaagagg catagagccg tccgttaatg aaattcgtga cgaacttggc
    10861 ttagttggga accatccgca attatcaatt ttgctggagg agtggtatca caatcaacca
    10921 gaatttaaac gaaaaagtca cacacctttg actgaaaata ttaatttaaa taccgatgaa
    10981 attcgcgaaa aaaatgtaga gcttgaaaaa tcaatttcgc tgttacgggc aacactcgaa
    11041 tccactgcag atggtattat gatggttaat ggccatggag ccgttgtcga ttggaatcaa
    11101 aagtttgtag aaatgtggcg aattccctct tatatgatgg agtcgggaaa agaaagtatc
    11161 agttttgaat acatacttga gcaacttatt gatccgcaat ctttgattgc tgatgtgcaa
    11221 tatctttatc aaaatctaga gtggcaaggt gaattacctg agcttcattt caaagacggt
    11281 cggatttatg agcgttttac tcagcctcag cgcgtgggct cacaaatagt ggggcgagta
    11341 tatagtttcc gtgatgttac tcaaaagagg atggctctcg atgagttgag aatacgtgaa
    11401 cgtgctattg aagccagcac acatggtgtg gtgattattg acgtaacaaa aaatgaaaac
    11461 aaagtcattt atgtcaatcg agctttcgaa agaataacgg gatatgggga gcaacatgct
    11521 ttagggaagg gtttgcttac tttgctaggt tcaaatttgg aggaggtaaa ccataaacgt
    11581 attgaactgg caattaggga aagcaaagaa gaaacaattg aaatggaaag tataaaacga
    11641 aatggagagt tctattggtg tgaaatcagc gttgctccgg ttaaagattc ttttggctat
    11701 gtaaaacatt atatttgcat tttaaatgat gtgactcaac gacgtgatat ggaagatcaa
    11761 ctgttgcagc aagcaactta tgattccctg actaatttac ctaaccgtgt cttactgatg
    11821 gatagagtgg agcaggccat tctacaagca agaaagaaca aggcaatttt ggctttttta
    11881 tttttggatt tagatcggtt taaactgact aatgatactt tgggacacag tatgggtgat
    11941 aaattattac aagccattgc caatcgatta ctcattgtta cagaggattt tgatactgtt
    12001 gccagattgg ggggagatga gtttgtgata ttacttacag acatagataa tatgctggaa
    12061 gctgaaacaa ttgcacaaaa tattttaaaa attatagaaa aacctattca aatagatcaa
    12121 catagtttga agattacagg tagtttggga atcagctttt atcccagaga tggtgatgat
    12181 tacgaatcat tgatgaaaag tgctgatttg tctatgtatc atgcgaaaga tactggaaga
    12241 aacaactatc gtgtctacga acctgaaatg aataggcgag ttattaatca tatgcaatta
    12301 gacaatgcgt taagagatgc tttaaaaaat gatgaattat ttctggttta tcaaccattg
    12361 attgacttaa agcaatccag agtagttgga ttcgaggctt taatgagatg gcatagcaaa
    12421 atattgggac ttgttagccc ggcggatttt atacccatgg cagaagaaaa tggcatgatt
    12481 ttggaaatgg gtgaatgggc aatgaagcaa gcttgcatgc aagtaaaaga atggcataaa
    12541 gctggtttta aaaacttaag tattgcggtg aatctttctg gacggcagtt tcgacaaaaa
    12601 aatttgccgg aggttgtatc gcgagtatta aaaagctctg gtttgcaatc gaggtttttg
    12661 gaactcgaat taactgaaag cctgttgata gaagatattg atcacgttgt agatactatg
    12721 tatgcattga aagacatggg aaccaagtta gtgattgatg attttggtac aggctactca
    12781 agcttgtctt atttaaaaca atttcccgta gataaactga aaattgatcg ttcgtttatc
    12841 actgaaatgg taagcaatca gaatgacgcg gctattgcca aagcgattat taacctgggc
    12901 cacagtttaa atcttcaggt tcttgcggag ggtgttgaaa atgaatttca aagagacttt
    12961 ataacatctc atggatgtga ctatgctcaa ggatattttt ttaaggctcc tgatactcca
    13021 gaaaatatat tagaattttt aaaaagttta tctgaatcga aaataaagta atgactaatt
    13081 tattaagtga agaattcctg ttttctctca aaactcaaat cccatcataa atctctccag
    13141 atcggtatgt tttttgcaag caaaggttaa attaatcaag tagtttctgt tggctttgtt
    13201 tattttatct tttatgttgt ttatatgaag ggcaagtata tagaacaact caaaatgaca
    13261 ggttgagcct gatttttact tttattcaag caatatattg attgaatttg tgtatttttt
    13321 aaggagattc ggaatagatg tcgtattgat atagaatggg ttgggaggat aaatgaagca
    13381 cattaccata attggagccg ggttagcggg aactttatgt ggtttatatc ttgctagaag
    13441 aggctatgaa gtagagttgt ttgaatcaag accagacatt cgtaacagtc cgactgatta
    13501 tggccgctcc attaatcttg ctttgtcgtg tagaggaatt accgctttaa aagcaatgaa
    13561 cctgttaagt gaagtaaata aaattatggt tccaatgcgg gcaagagcta ttcacgaagc
    13621 caatggcgag gttcactatc aaccatttgg acgtcacatt gatgaatata ttaatgctat
    13681 ttctcgttct gatctcaatg ccttgctttt aaataaagcg gaattatgcc ctaacattaa
    13741 attacacttt aatatgaaat tgcactctct tgatattcat aataaaaaaa ttaaatttga
    13801 aaataaaaat ggtgattttg ttgaggtctc ataccataga ctaattggag cagatggtgc
    13861 gccgtctcat gtaagggata tgctgaaaaa tgagggaata gttagtgcaa gcagagattt
    13921 tttatctcat ggatacaagg agctttcaat tagcaaaaaa catacaaaag ggatggctag
    13981 agagcatttg catttatggc cacgtgattc gtttatgcta ttaggtaacc ctaatcctga
    14041 cgattcaatt acaggttctt tgtttttagc taatgaaggg aaagacagct ttgctgaact
    14101 aaataatgaa gaaaaattac atttattttt taaaacacag ttcccggatg cctatgcagc
    14161 tatgccaaat ttggttcaag aattttttgg taatcctact ggacatttga gtactatcca
    14221 atgctcaccc tggtactata aagacgaatg cttattaata ggtgatgcgg cacatgggat
    14281 aattccattc tttgggcaag gaatgaatag cgcatttgaa gattgccgta ttcttgatga
    14341 attactggat gaataccaag acgactggtc gcgagtgaac cctgtttttt atgaacacag
    14401 aaaagtgaat accgatgcga tagcgaaaat gtctatggat aattatcatg aaattcattc
    14461 ggacataaga aatccaaaat ttattttaca aaagcagatt gaacgggagc tcatgttgcg
    14521 atacccggaa cattatgttt ctatgcatgt tttggttatg tttaccaata ccccttatgc
    14581 caaggcgatg gcaataggcg agttacaatc tggcctttta gagcaaattt gtttcccaat
    14641 aactgatatt aaggagttaa attggcagga agtagagaaa ttacttagcg tatatgacaa
    14701 aaaattggcg aaaattattt ga
//
LOCUS       DASLOE010000066        14640 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.066, whole genome shotgun sequence.
ACCESSION   DASLOE010000066 DASLOE010000000
VERSION     DASLOE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14640
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.066"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(243..1667)
                     /gene="lpdA"
                     /locus_tag="U6309_001584"
     CDS             complement(243..1667)
                     /gene="lpdA"
                     /locus_tag="U6309_001584"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEN5505025.1"
                     /translation="MANKIKTDVVVLGSGPGGYTAAFRAADLDKKVVLVERFDSLGGV
                     CLNVGCIPSKALLHIAKVVDEAHEMSEQGVAFGKPKFDNKKLVAWKNSVVAKLTGGLK
                     ALAKQRKVEVITGTGKFSGTHQILVETKEGTVEIEFDNAIIAVGSESIKLPFIPEDKR
                     IFSSTGALELSDIKGDLLVLGGGIIGLEMATVYSSLGVNVTVVEFMDQLIPNADADLV
                     NILQKRMTKKGVKFLLKTKVTAVEAKKDGIYVSMEGEHATDKPLCFQQVLVAVGRKPN
                     GGAINAEKAGIKVDERGFIPVDNQLRTNVPYIFAIGDVNGQPMLAHKAIPEGKVAAEV
                     IAGKKHYFEPKCIPSVAYTDPELAWAGLTEKEAKEKGINYEKASFPWAASGRALSMGR
                     EEGMTKLLFCPETNRILGAGIVGVNAGDLIAETALAIEMCCDVEDIALTIHPHPTLSE
                     TIAQSAEAFEGTITDLYLPKKKKK"
     gene            complement(1756..3390)
                     /gene="aceF"
                     /locus_tag="U6309_001585"
     CDS             complement(1756..3390)
                     /gene="aceF"
                     /locus_tag="U6309_001585"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEN5505026.1"
                     /translation="MTKESEIKIPDIGGANQVDVIEILVKEGDQIEVDTPLITLESEK
                     ASMDIPSPISGTVTQILVKVGDKVSEGDLIVKAKSDTTTNISSSQEQKTELEKQNSQT
                     RPEEQSVDTKATASTPDSKDIEISIPDIGGANDVDVIDILVKPGMEVEKDQALITLEG
                     DKATMDIPSPYAGKVIEMKIKLGDKVSQGTPILTLKTPGKSETPEIEKSQIKNISEQS
                     IKEIEKPYEELKSEPISINNLEIAESKSILISAGPAVRRLAREFGVDLSLVQGSGRKS
                     RITKEDLQNYIKVRLNEKTTSGGFSLPSNPAIDFSKFGSIETKPLNKIKKLTGANVHR
                     SWITIPHVTQFDEADITDLEAFRKSESESTKNQDYKLTLLAFVCSVVCKALHAYPQFN
                     ASLDASGENLIYKKYYNIGIAVDTPNGLVVPVIKNVDKLSVIDIAKEMSRLSTKAREK
                     GLTPIDMSGGCFTISSLGGIGGTAFTPIVNSPEVAILGLSRSVIKPIYDNKEFKPRLM
                     LPISLSYDHRVIDGAEAARFTRFLCDCLGDIRRILL"
     gene            complement(3448..6111)
                     /gene="aceE"
                     /locus_tag="U6309_001586"
     CDS             complement(3448..6111)
                     /gene="aceE"
                     /locus_tag="U6309_001586"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEN5505027.1"
                     /translation="MTNETNLDLDPIETREWLDALQAVLFNDGPERTAFLLQQLLNKA
                     NAEGVKLTSSINTPYRNTIKPHEEKQMPPDEGIGKRINALIRWNAVAMVLRAGKYAPE
                     LGGHIASYASASTLYETGFNYFFKGQKGENGGDLLYIQGHSAPGIYARAFLEGRLTEK
                     QLEKFRQEVEVDGLSSYPHPWLMNDFWQFPTVSMGLGPLQAIYQARFLKYLENRGLIK
                     AEGRKVWAFLGDGEMDEPESVGALSIAAREKLDNLIFVVNCNLQRLDGPVRGNGKIIQ
                     ELEGLFRGAGWNVIKVIWGGRWDPLFARDNQGWLQKRMEECLDGDYQSYKANDGSYVR
                     QHFFNQYPELKKMVENMSDEEIWRLNRGGHDPQKVYAAYARAVEHKGTPTVILAKTIK
                     GYGMGAAGEGQNITHQQKKMTIDQLKAFRDRFNIPISDDKIADIPFYKPDDDSPEIKY
                     IKKQREALGGYLPHRSTEVEQLKIPDLGEFSGITKGLGDREISTTMAFVRILSVLLKN
                     KDINSRIVPIVPDECRTFGMEGLFRQIGIYSPVGQLYTPVDHEQVMYYREAVDGQILE
                     EGINEAGAFCSWIAAATSYSSNKLAMIPFYIYYSMFGFQRIGDLAWAAGDMQARGFLL
                     GGTAGRTTLAGEGLQHQDGHSHVLASTIPNCISYDPTYAYELAVIIQNGLHRMYEKQE
                     NVFYYITIMNENYSHPDMPAGVEEGIIKGMYLLKENKKKSKNHVQLMGCGTILREVIK
                     AAEMLEEDFSITSDIWSVTSFNELRKEGLAVERYNNMHPQNKPQESYVTSQLKGRRGP
                     VIASTDYMRIYADQIRPFVPNRYITLGTDGYGRSDTRTQLRHFFEVDAKFIVLAALNA
                     LVAEGSLDKAKIVDAMKRYNINQDKLDPMTH"
     gene            complement(6139..6528)
                     /locus_tag="U6309_001587"
     CDS             complement(6139..6528)
                     /locus_tag="U6309_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN5505028.1"
                     /translation="MNTAFRLLFCLIILELSACATLKSKITHHKTLSQCQQTCFQQLN
                     YCKQNCTDNCRDCSTKANHFAKENYLEYLHEVKIQGGYITRGLQSYRDPLQCRKVTCN
                     CSADFNACNQGCSGVIQKRLQPVPYCS"
     gene            complement(6567..7319)
                     /locus_tag="U6309_001588"
     CDS             complement(6567..7319)
                     /locus_tag="U6309_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505029.1"
                     /translation="MKLLVIVLCLLSERFLIHSVSYQRFSWFNNYCLFLKKFIDKNKY
                     FSNPWATLIAIILPIVLLTFLVYFSLQSILFGLFGLILSLFIFYYCLGPQNAFYPILK
                     KQANQTETDAIGEYFAEINSQLFAVIFWYIIAGPIAALTYRLIALCKQINFISTQASQ
                     ITNILEWIPARITALLFLLVGNFQRGFHLFVQYVLTSPDSNDKILRGCGLQAVRINDT
                     EEVPMAAAENLVEHATIVLLVFIALFTLVAWL"
     gene            complement(7324..8496)
                     /locus_tag="U6309_001589"
     CDS             complement(7324..8496)
                     /locus_tag="U6309_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN5505030.1"
                     /translation="MHDGSVFYTIFLIFAGAAFFSTLVLYTKQSLLVAYITLGAILGP
                     WGFKLVSDVTVVKQIGDIGIVFLLFLLGLHLQPQNLIHMLRKVTWIAIVSSILFAALA
                     YGIGRWFGLTVTESLILGGAMMFSSTIIGLKLLPTTILHHQHTGEVMISVLLMQDVIA
                     IILLILLNSAHQDGNLSINDFILVIIALPALCLFSFLFERYILIKLLARFDRTQEYVF
                     LLSIGWCLGISVLAQTIGLSEDIGAFIAGVALASSPISLFIAESLKPLRDFFLVMFFF
                     SIGATFNFGFGAQVVIPAVILATLILIIKPLLFYVLLARSGEKKQVAKEVGIRLGQGS
                     EFSLLVASIALSTNLISEVASNLIQATTILTFIVSSYLVVLKYPTPIALSEKMRKD"
     gene            complement(8816..9637)
                     /locus_tag="U6309_001590"
     CDS             complement(8816..9637)
                     /locus_tag="U6309_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEN5505031.1"
                     /translation="MRKLLIIFSIFLASCQTNHDKPNLTNKKHQTTHTKNSVYDRYKY
                     KSNRYTIQQDGAPKQAKQVSFKEPVPVKEPLSRYGNPSEYYVDGRTYEVMKSSTGYKT
                     RGIASWYGTKFHKQRTSSGEPYNMYVMTAAHKTLPLPTYVKVRNLENGKVAIVKVNDR
                     GPFHANRVIDLSYAAALKLGVFPKGTAHVEIETLMGPAGQGHYYLQAGAFSSEVLAKR
                     LKEKLIHITPSPVFIEHYKQHYIVRVGPFGNKSMADNLKTKLSNNGIKGSFSVLI"
     gene            9833..11056
                     /locus_tag="U6309_001591"
     CDS             9833..11056
                     /locus_tag="U6309_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN5505032.1"
                     /translation="MTRATSFLLTLLFFITLFVQSTNSMADEVLPSSDLQKPSPTVTT
                     KPFTTPAAPLLNAKAYILIDVNSGKVIAEKNSEEKLPPASLTKMMTLYVISNALKSGQ
                     IHLNDMVRVSRDAWQTGGSRMFIKEGDQVSVEDLLKGIIVDSGNDACVAMAEHLGGSE
                     NAFTDIMNQQAQNLGMKNSHFTDSTGLPDPNLYTTAKDLAILGRALILDFPEYYDWYK
                     QKWFTYNGIRQPNRNRLLWRDSQVDGIKTGHTNDAGYCLVSSAKRNNMRLLAVVLGEP
                     SDSVRADDSEKLLNYGFRFFETHQLYKSGQSIAELPLYKGTSDKVAVGLIEDQFITIP
                     AGQYERLNISTKISNDLQAPIKKGDKVGDIIVQFNNNIVSSKPLYALQTVESGGFYTR
                     TKDSIRLAFKRWFGS"
     gene            11069..11905
                     /locus_tag="U6309_001592"
     CDS             11069..11905
                     /locus_tag="U6309_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEN5505033.1"
                     /translation="MNIAFVNGKYCSQSEAKVSIFDRGFLFGDSVYEVLPVYHGQPYF
                     VDQHLDRLFSNMKKIKMTIPNYDWHGLIHRLISENNGGNLQVYIQVTRGNQGVRKHDI
                     PTSITPSVIAFTMHNSFPTLEDKEQGMSAKLVEDFRWMRCDIKTTSLIANILLNDEAV
                     SSGFHTAILARNGLITEGSSNNVFIVTQDGVIKTPPMNNFCLPGITRQVVIEIIKKLD
                     LKFREIEISMSELFSAQEVWITSTTKEVFPITKINDSLINGGKVGEYWRIINDSYQQL
                     VN"
     gene            11909..12172
                     /locus_tag="U6309_001593"
     CDS             11909..12172
                     /locus_tag="U6309_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEN5505034.1"
                     /translation="MTKTTLIEFPCYFPIKIIGINSELLLNEIRQIVINHFPTFENEH
                     LTHKTSEQNKYLAITVTVYAENQISLDGLYQDLSQHPAVKMVL"
     gene            12172..12771
                     /gene="lipB"
                     /locus_tag="U6309_001594"
     CDS             12172..12771
                     /gene="lipB"
                     /locus_tag="U6309_001594"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEN5505035.1"
                     /translation="MIIHNLGIKDYTETWEQMKEFTAIRDSNSCDELWLLEHYPVYTQ
                     GQAGKPEHVLNPNSIKIVQSDRGGQVTYHGPGQLVAYVLMDIRRRNLGIRTLVVKLEE
                     ILISVLEHYGIPANIRSGAPGVYVGEKKIASIGLRVKNGCTYHGIALNVNMDLSPFLG
                     INPCGFAKMEMTQMSHFHPNIQLEEVSQHFVQYFLTQFK"
     gene            12781..>14640
                     /locus_tag="U6309_001595"
     CDS             12781..>14640
                     /locus_tag="U6309_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEN5505036.1"
                     /translation="MNLLADYVQPLTYWLQANPHWSLFITFLIALSESLAVIGSIIPG
                     SVTMTAIGILAGSGIMRIDLTLLAATLGAICGDSLSYFLGYFYSDRLIEIWPFRKYPG
                     LLKYGKDFFASHGGKSVLIGRFVGPLRSIIPVIAGIMHMKQWRFLIANIVSAIGWSIL
                     YVMPGVLIGAAGHELSAEGATRFFILILVILAVIWLSSLVLKWLFIKLNSFLRNNLHR
                     FWLEIKTSPRLSKVYYAITPVQEENHYNTVGLLLIALVIFFLFLTLVIFTIEGQLLDY
                     INVPVHFFMQSFHSSLLEAVFIVCAQLTSKSTLAGLFIASCIWFIYHKNYRATVYLSS
                     ITLLSGVIALFLTKIIDSPRPQGLLVTLPGSSFPSVSISIATALYGFILFYINSKYSL
                     ITSSFKTVILIVLGLSGFANIYLGDFWFSDILAAYLLGAEVCLIHCLVYRRFSTSAIK
                     NVHPLIMLISLLMVIVFATAVSTVVNFKELVHDHMPYHKEFTLTEKQWWDQQRPLLPL
                     YRLSRIGKRISLLNIQYVGDLDALQNKLEQFGWEPQSDKTFFTRLLMRMNSAPSNVKL
                     PLLTQLYENKKPGLIMTYQDKKSKLIFDLTIWQSNYYLSEPKRMVWIGSIHQNI"
BASE COUNT         4405 a         2754 c         2675 g         4806 t
ORIGIN      
        1 taattcttta gtcttttatt tagagccttt gcatgactgt cctttcattt ttttgctgtg
       61 ctgcagatat ttttgtggga tgtatgtaaa ctctactccg caaaaaaatt tgtatctagc
      121 ccaaaataaa ataatcagtc catgcaagac gtctatatat aattatatac atataataag
      181 catttccttt gcttgtataa gcaaaggaaa tgcaatgagg tataattggc tacctgtaat
      241 tattattttt tcttcttttt gggcaaatac agatccgtta tagtgccttc aaatgcttct
      301 gctgactgag ctatggtttc tgatagagtc gggtgagggt gaatagtaag agcgatgtct
      361 tcaacatcac aacacatttc aatggctaag gcagtttctg caattaaatc gccggcatta
      421 accccaacaa tacctgctcc caggatacgg ttagtttctg ggcagaatag taatttggtc
      481 attccttctt ctcgccccat gctgagtgca cgaccgctag cagcccatgg aaaggaagct
      541 ttctcatagt ttattccttt ctcttttgct tctttttctg ttagaccagc ccaagctaat
      601 tctggatctg tataagcaac actaggtata cattttggct caaaataatg ctttttccct
      661 gcaatgactt ctgctgcaac tttcccttca gggatggctt tatgtgcgag cattggttgt
      721 ccatttacat cgccaatagc aaaaatataa ggaacattcg ttcttaattg attatcaact
      781 ggaataaagc cacgttcatc tactttgatc cctgcttttt cagcgttgat tgctccgccg
      841 ttaggttttc tgcctactgc aacaagtact tgctgaaaac aaagtggctt atcagttgca
      901 tgttctcctt ccattgaaac ataaatacca tcttttttgg cttcgactgc agttactttc
      961 gtttttaata gaaatttgac acccttttta gtcattcgtt tttgcagtat attgaccaag
     1021 tcggcatcag cattcggaat taattggtcc ataaattcaa caactgttac attaaccccc
     1081 agcgaggagt agacagttgc catttccaag ccaataatac caccacccaa tacaagtaag
     1141 tcacctttga tatcagagag ttctaatgct cctgtagagc taaatattcg tttatcttct
     1201 ggaataaatg gtaatttaat ggattctgag cctactgcaa taatggcatt atcaaattca
     1261 atttctacag tcccttcttt cgtttcaaca agaatttgat gggtacctga aaacttacct
     1321 gtcccagtaa ttacttctac ttttctttgt ttggcaagag cttttagtcc cccggtcaac
     1381 tttgcaacga cagaattttt ccaggcaacc aattttttat tgtcgaattt gggtttacca
     1441 aatgctacac cttgctccga catttcatgg gcttcatcta ctactttagc aatatgcaat
     1501 aatgcttttg agggaatgca accaacattt aaacaaaccc cacctagtga gtcaaaacgt
     1561 tcgactaaaa ccaccttttt atccaagtcg gcagctctga aagcggctgt gtatccgcct
     1621 ggtccactac ctaataccac tacatctgtc ttaattttat tagccataag aagcctcatt
     1681 aaaaaatatg ttttctgggg ttattggcat ttcattcaga acaaggaaat accaatagct
     1741 ctattccttt atatgtcaga gtaaaattct tctaatatca cctaaacaat cacaaagaaa
     1801 gcgagtaaac cgggctgcct cagcgccgtc gattactcta tgatcgtaag aaagggaaat
     1861 aggcaacatc aatctgggct taaactcttt attatcgtat atgggtttta ttactgatct
     1921 tgataagccc aaaattgcaa cttccgggct atttacaata ggagtaaatg ccgttccgcc
     1981 tataccacca aggctggaaa ttgtaaaaca accgcctgac atatcaatag gtgtcaatcc
     2041 cttttctcta gctttcgtac tgagtctgga catttccttt gcaatatcaa tcacactcaa
     2101 tttatcaaca ttctttatta caggaaccac cagaccgtta ggtgtatcta ctgctatacc
     2161 tatattataa tattttttat aaattaaatt ttcaccactg gcatccagtg acgcattaaa
     2221 ttgcgggtaa gcatggagag ccttacaaac tacgctacaa acaaatgcta atagagttaa
     2281 tttgtaatct tgatttttag tgctctctga ttctgatttt ctaaacgcct ccaggtctgt
     2341 aatatccgct tcgtcaaatt gagtgacatg tggaatggtt atccaagagc gatgtacgtt
     2401 tgctccagta agctttttaa ttttatttaa aggctttgtt tcaattgaac caaatttact
     2461 gaaatcaatg gctggatttg acggtaagct gaagccgcca gaagtggttt tttcatttaa
     2521 acgtactttg atataatttt gcaggtcttc ttttgtaata cgagattttc tcccacttcc
     2581 ctgaaccaag gataaatcga ctccaaactc tcttgccaat cttcttacgg ctgggcctgc
     2641 agatattaag atactttttg attcagctat ctcaaggtta ttaatcgata ttggttcgga
     2701 ttttagttct tcatatggct tctctatttc ctttatagat tgttctgaga tatttttaat
     2761 ctggcttttt tcgatttcag gtgtctcaga ttttcctggg gtttttagag ttagaatagg
     2821 agtaccttga gatactttat cccctaattt aattttcatt tcgatgactt tacctgcata
     2881 gggcgaagga atatccatcg ttgctttatc accctctaat gtaattaatg cctgatcctt
     2941 ttctacttcc atccctggtt tgactaaaat atcaattaca tcaacgtcat ttgcaccacc
     3001 aatatccggg atagatattt caatatcttt agaatcaggt gtagatgcag ttgcctttgt
     3061 atcaacagat tgctcttctg gtctggtttg cgaattctgt ttttctaatt cagtcttttg
     3121 ttcttgagaa ctggaaatat ttgtcgttgt gtctgacttg gctttgacaa tgagatcgcc
     3181 ttcggatact ttgtcaccta ctttaactag aatttgcgtt accgttccag atattggaga
     3241 agggatatcc atgcttgctt tctcagactc aagcgtaatc aacggtgtat ccacttcgat
     3301 ctgatcaccc tctttcacta aaatctcaat gacatcaact tgattagcgc caccgatatc
     3361 aggaatttta atttcacttt cttttgtcat attttcctca gcactcagtt aaaccggaac
     3421 tattttctgt tctgattaat tgtttaacta atgggtcatt ggatccaatt tgtcttgatt
     3481 aatattgtaa cgcttcatgg catcgacaat ttttgctttg tcaagactac cttcagctac
     3541 aagtgcattt aatgccgcta acactataaa ttttgcatca acttcaaaga aatgacgtag
     3601 ttgtgtacgg gtatcacttc tgccgtaacc gtctgtaccc agggtaatat agcgattagg
     3661 aacaaaaggc ctgatctgat ctgcataaat acgcatatag tcagttgagg caataacagg
     3721 tccacgacgt ccttttaatt gagatgtcac ataactttcc tgaggtttat tttgggggtg
     3781 catgttattg tatcgctcta cagccaaacc ttctttacgt aactcattga aactggttac
     3841 actccagata tccgatgtaa ttgaaaaatc ctcttccagc atctctgcgg ctttgataac
     3901 ttcacgcaaa atagtaccgc atcccattag ttggacatgg ttttttgatt ttttcttatt
     3961 ttcttttaag agatacattc ctttgataat accttcctct actcctgcag gcatatctgg
     4021 atgagagtag ttttcattca taatagtaat gtaataaaac acgttttcct gtttttcata
     4081 catacggtgc aatccatttt ggatgataac tgctaattca taggcgtatg taggatcata
     4141 agaaatacaa ttaggtattg ttgatgctaa aacatgacta tgtccatctt ggtgttgcaa
     4201 tccttctcct gccaacgttg ttcggccagc ggttccaccg agcagaaatc ccctcgcttg
     4261 catatctcct gccgcccatg ctaaatcacc aatgcgttgg aaaccaaaca tagagtaata
     4321 aatgtaaaat ggaatcattg ccagcttatt tgaactgtaa gaggttgcgg cagcaatcca
     4381 tgaacaaaac gccccagctt cattaatacc ttcttcaaga atctgcccgt caactgcttc
     4441 tctgtaatac atcacttgtt catgatctac gggagtatag agttgaccta ctggggaata
     4501 aattccaatt tgtctgaata atccctccat accgaatgtt ctgcattcat ctggtactat
     4561 gggaactatt ctggaattaa tgtctttatt tttgagtaga actgaaagaa ttcgcacaaa
     4621 agccatagta gtcgagattt ctctatctcc taatcccttg gtaataccgg aaaactctcc
     4681 taaatcaggt atttttaact gttctacttc agttgaacgg tggggcaaat aaccgcctaa
     4741 ggcttccctt tgctttttta tatacttgat ttcgggactg tcgtcatcag gtttatagaa
     4801 aggaatatca gcaattttgt catcgctgat tggaatatta aagcgatctc tgaaagcttt
     4861 tagttgatct atggtcattt ttttctgctg atgagtaatg ttttgtccct caccagccgc
     4921 acccatcccg tagcccttga tagtttttgc aaggataact gttggagttc ccttgtgttc
     4981 aactgctctg gcataagctg catacacttt ttgaggatca tggccaccac gatttaatcg
     5041 ccaaatttcc tcatcagaca tattttcaac cattttcttt aattctggat attgattaaa
     5101 gaaatgttgt cttacatacg atccatcatt cgctttgtaa gattgataat ctccatccag
     5161 acattcttcc atccttttct gtagccagcc ttgattatca cgggcaaata atggatccca
     5221 acggcctccc cagatgacct taataacgtt ccatccagct cctcgaaata ggccttcaag
     5281 ttcttgaatg attttaccgt taccacgcac aggcccatca agtctttgta aattgcaatt
     5341 aactacaaaa atgagattat ccagcttttc tcgtgctgca atactgagtg ccccgactga
     5401 ttcaggctca tccatctcac catcacccaa gaatgcccat actttcctac cctcagcttt
     5461 gatcagtcct cggttctcaa gatattttag aaagcgagct tgatatattg cttgtagagg
     5521 gcctaaaccc atggatacgg tgggaaattg ccaaaaatcg ttcattagcc aagggtgtgg
     5581 gtaggaagat aaaccatcca cctctacttc ttgccggaat ttttcaagtt gtttctctgt
     5641 taatcggcct tcaagaaatg ctctggcata aatgcctgga gccgagtgac cttgaatata
     5701 aagcaagtcc cctccgtttt cgcctttttg tcccttaaaa aaatagttaa atccagtttc
     5761 atataacgtt gatgcagacg cataggaagc gatatggccg ccaagttctg gagcgtattt
     5821 acctgcccta agtaccattg caacagcatt ccatctaatt aatgcattga tgcgctttcc
     5881 aattccttca tcgggaggca tttgtttttc ttcgtgaggt tttatagtgt ttctgtaggg
     5941 agtattaatt gagctggtta gtttaacccc ctcggcgttc gctttattaa gaagttgctg
     6001 caaaagaaat gcagtacgct caggaccatc attaaagagt acagcttgta gggcatccag
     6061 ccattcacgc gtttctattg gatccagatc taaatttgtt tcattggtca tgaaatagtt
     6121 ccccaacaat caataatttt atgaacagta tggtacgggt tgtaatcttt tctgaattac
     6181 accagaacaa ccttgattac atgcattaaa atccgcagag caattacaag ttactttgcg
     6241 gcattgtagt ggatcacggt aagattgcaa cccgcgagta atatatccac cttggatttt
     6301 gacttcgtga aggtattcca gataattttc tttggcgaaa tggtttgctt tcgtagaaca
     6361 atctcggcag ttgtctgtgc aattttgttt gcagtaatta agctgctgaa aacaagtttg
     6421 ttggcattgg cttaaggttt tatgatgggt aattttactt ttcagtgtgg cacaagctga
     6481 aagctccaat attataaggc aaaaaagtaa acggaatgca gtgttcatga ttcaagtccc
     6541 acattaattt attgtgaaat aaaacattat agccaagcta ctaacgtaaa taatgcaata
     6601 aaaaccaaca acactattgt ggcatgttct acaagatttt cagcagcagc cattggtact
     6661 tcttcagtat cattgatacg aactgcttgc aaaccacacc ctctcagtat tttatcattt
     6721 gagtcaggag aagttagaac atattgaacg aatagatgga aacctcgttg aaaatttcca
     6781 actaacaaaa acagcaaagc ggtgattctg gctggtatcc attcgagaat gttagtaatt
     6841 tgacttgcct gagtacttat aaaattaatt tgtttacata aagcgattaa tctgtatgtt
     6901 aaagctgcta ttggcccagc gatgatgtac cagaatataa cagcaaataa ttggctgtta
     6961 atctcagcaa aatattctcc aattgcatct gtttcagttt gattagcctg tttcttcaaa
     7021 attgggtaaa aagcattttg cggccccaga cagtagtaaa aaataaacag gctaagaatt
     7081 aggccaaata aaccaaacag aatactttgt agtgaaaaat aaacaagaaa tgtgagaaga
     7141 acaatgggca atatgatagc tatcaaggta gcccaaggat tagagaaata tttattctta
     7201 tcgatgaatt ttttaagaaa tagacagtag ttattaaacc aagaaaatcg ctgataggat
     7261 actgaatgga ttaaaaaccg ttcactgagc aagcataata ctattaccag taatttcatt
     7321 tgattaatcc ttacgcattt tttcagataa agctataggt gttggatatt tcaatacaac
     7381 aagatacgat gaaacgataa aagttaaaat agtagttgcc tggattaaat tggaggcaac
     7441 ttctgaaatc aggtttgtac tcaaggcgat gctcgcaacc aataaagaaa actcactccc
     7501 ctgccccaag cgaattccta cttcttttgc tacttgtttt ttctcaccag aacgggcaag
     7561 caatacataa aatagcaacg gcttaataat cagtatcaat gttgctaata tcactgctgg
     7621 gataactacc tgagctccaa atccaaaatt aaaagtagca cctatcgaaa aaaagaacat
     7681 tacaaggaaa aaatctctta atggctttaa actttcagca atgaacaatg aaattgggct
     7741 tgatgccaaa gctactcctg ctataaaagc gccaatatct tctgataagc ctattgtttg
     7801 tgctaaaacg gatatcccca aacaccatcc tattgagagt agaaaaacgt attcctgagt
     7861 tctatcaaag cgtgccagca gttttatcag gatgtatctt tcaaataaaa aagagaacaa
     7921 gcacagggca ggtagcgcga taataaccag gatgaaatca ttgattgaca ggttcccatc
     7981 ctgatgggca ctattaagta atatcaaaag aattatggct ataacgtcct gcattaagag
     8041 tacgctaatc atgacttcac cagtatgctg gtgatgtaaa atggttgttg gtaataattt
     8101 caagccaata atagtacttg aaaacatcat ggctccgccc aatatcaatg actcagtcac
     8161 cgtgaggcca aaccatctgc caattccgta ggcaagtgca gcaaacagaa ttgagcttac
     8221 gattgcaatc caggttactt ttcttagcat atgaatcaaa ttctggggct gtaaatgcaa
     8281 acctaataag aaaagcaaga atactattcc aatgtcacct atctgcttga caaccgtcac
     8341 atcggataca agtttgaatc cccagggacc taagatagca ccaagtgtta tataagcaac
     8401 caataatgac tgtttggtgt acaataccaa ggtagaaaaa aaagcagctc cagcaaatat
     8461 cagaaatata gtgtaaaata cagagccatc atgcattgat tgaatacctc ttaagaaaag
     8521 gtagttaaaa ttgaattata aagccaattt tatccattaa tctattaatt agctattaat
     8581 tattgtttat taataataaa ttgttgcctt tagttagaac caaggtgata tctattgata
     8641 agaatgtcat cgatagagtc ataatgtgct ggtaaaatta acacaagcta tgcacacatt
     8701 cagaatcagt ctgagtataa tgttacacct aaaactattt ctttcccaaa ctattctgag
     8761 tggttttttc ttaattctaa agatataaat taaccagttt tttccacgaa acaaattaaa
     8821 ttaacacaga aaaggatccc tttattccgt tatttgataa ttttgtttta aggttgtcag
     8881 ccattgattt gttaccaaaa ggccctactc ggacaatata gtgctgttta tagtgttcaa
     8941 taaacaccgg cgacggtgta atgtgaatta gtttctcttt caaacgcttt gccaggactt
     9001 ctgaggaaaa ggcccctgcc tgcaaataat aatggccttg cccagcaggc cccattagtg
     9061 tttcaatttc aacatgagca gttcctttgg gaaaaacccc cagttttaat gcggcagcgt
     9121 acgagagatc tatgacacga tttgcgtgaa atggccctct gtcattaacc ttaactattg
     9181 cgactttgcc attttccaag tttcttactt tgacataagt cggcaaaggt aatgttttgt
     9241 gggcagcagt catgacatac atgttatagg gttcaccact ggaggtacgc tgtttatgaa
     9301 attttgttcc ataccatgag gcaataccac gagttttata ccctgttgag cttttcatga
     9361 cttcataagt gcgcccgtca acataatatt cagaaggatt accatatctg cttaatggtt
     9421 ctttgacagg cactggttct ttaaagctta cctgtttggc ttgtttggga gctccatctt
     9481 gctgtattgt ataacgattg gacttgtatt tatatcgatc gtagacagag tttttagtgt
     9541 gagtggtttg gtgtttttta tttgttaggt tgggtttgtc atgattagtt tggcaacttg
     9601 ctaaaaaaat tgaaaaaata ataagtagtt tacgcatatt ctctgatatt tcttgttgtt
     9661 atgagttatt gctaatatta ccaaaaaata ataattttcg aagtgcgatt aaattaattc
     9721 acaacttatt tagctttata aaaaaaatat taatattttt aatatgccat ttggccgcct
     9781 gcaaaaggct gtgataagat acatttattt tgcaactata tcaggtaata tcatgaccag
     9841 agcaacgtct tttctattga cgctattatt ttttattact ttatttgtac agtctaccaa
     9901 ttcaatggct gatgaagtgc tcccctcctc tgatttgcag aagccttctc caacagtcac
     9961 aacgaaacct tttacaactc cagctgctcc attgcttaat gccaaagctt atattttaat
    10021 tgatgtgaat agtggaaaag taattgcaga aaagaatagt gaggaaaaat taccacctgc
    10081 cagtttgacc aaaatgatga cattgtatgt catttctaat gcattgaaga gtggacaaat
    10141 tcatcttaat gatatggtaa gagtgagccg tgatgcatgg cagacaggcg gctctcgtat
    10201 gtttataaaa gagggtgatc aggtctctgt tgaagacctt ttaaaaggca ttatagtaga
    10261 ctctggtaat gacgcttgtg tcgcaatggc agagcacctt ggtggttccg aaaatgcttt
    10321 tacagacatt atgaatcagc aagctcagaa tctgggtatg aaaaatagtc attttacaga
    10381 cagtactgga ttacctgatc ctaatctata tacaactgct aaagatttag cgattttagg
    10441 acgagcctta atactcgatt tccctgagta ttatgactgg tataaacaaa aatggtttac
    10501 atataacggt atacgccaac ctaatagaaa tcgtttgttg tggcgagata gtcaagttga
    10561 cggtataaaa acaggccaca ccaatgatgc tggatattgt ctagtatctt ccgcaaagcg
    10621 taataacatg cgtttattag ctgtggtttt aggggaaccc agtgattctg tgcgtgctga
    10681 tgacagtgaa aaattactaa attacggatt tagatttttt gaaacacatc aattatataa
    10741 atctgggcaa tcaattgccg aattaccgct ttacaaagga acttctgata aagttgctgt
    10801 gggtttaata gaagatcaat ttattacgat acctgccggt caatatgaaa gattaaatat
    10861 ttcaactaaa atttctaatg atttacaagc tcctataaag aagggcgata aagttggcga
    10921 tattattgtg caatttaata acaatatcgt ttcaagcaaa ccgctttatg ctttgcaaac
    10981 agtggagtca ggtggattct ataccagaac aaaagattcg ataaggttag catttaagcg
    11041 atggttcggc tcctaggata ttttcataat gaatatcgca tttgttaacg gcaagtattg
    11101 ttctcaatct gaagcaaaag tttcaatatt tgatcgaggt tttctttttg gtgactcagt
    11161 ttatgaagtg ctgcctgttt accatgggca gccttacttt gtagaccaac atcttgaccg
    11221 attattctca aatatgaaaa aaattaagat gactatacca aattatgatt ggcatggttt
    11281 aattcataga ctaatatcag aaaataatgg aggtaattta caagtatata tccaagtcac
    11341 acgaggaaat caaggggtgc gcaagcatga tatccctact tccatcacac cttctgttat
    11401 cgcattcacc atgcataatt catttcccac cctcgaagat aaggaacagg gaatgtcagc
    11461 aaaactggtt gaagattttc ggtggatgag atgtgatata aaaactactt ctttaattgc
    11521 caatatatta ctgaatgatg aggctgtatc ttcaggattc cacactgcaa ttcttgcccg
    11581 gaacggtcta attacagagg gaagtagtaa caacgtattc attgtcacac aggatggtgt
    11641 tattaagacc ccacccatga ataatttctg tttaccagga attactcggc aagttgttat
    11701 tgaaataatt aaaaaattag atttaaagtt cagagaaata gaaattagca tgtcagagct
    11761 tttttctgct caggaagttt ggataacaag tacgacgaaa gaagtattcc ctattacaaa
    11821 gattaatgat tctttgatta atggcggaaa agttggcgaa tattggcgga taattaatga
    11881 ttcctaccaa caactagtaa actaaattat gacaaaaacg actttaatag aattcccttg
    11941 ctattttcct ataaaaatta tcggaattaa ctcagaatta ttgctcaatg aaatcaggca
    12001 aattgttatt aatcacttcc ctacttttga aaatgagcat ttaactcata aaacgagtga
    12061 acaaaacaag tacttggcta ttactgtaac ggtgtatgca gaaaatcaaa tctcgctgga
    12121 tggattatat caggatttaa gtcaacatcc tgctgtgaaa atggttttat aatgataatt
    12181 cataatttag gcataaaaga ttatactgaa acatgggagc agatgaagga attcactgca
    12241 atccgagata gtaattcttg tgatgagctt tggttattag agcattatcc tgtatatact
    12301 caggggcagg cagggaaacc agagcatgta cttaatccca actctataaa aattgtgcaa
    12361 tcagatcgcg gcggacaagt tacatatcat ggcccaggtc aactagtagc ctatgtttta
    12421 atggatattc gtcgcagaaa cctgggcata agaactttag tagtaaaact ggaagaaata
    12481 ttaatttcgg ttttagaaca ttatggaatt ccagcaaaca ttcgcagtgg ggcaccaggg
    12541 gtttatgtag gtgaaaaaaa aatcgcatcg ataggactga gggtgaaaaa tgggtgtacc
    12601 tatcatggta tagcattaaa tgtgaacatg gatttgagcc catttttagg cattaaccca
    12661 tgtggttttg ctaaaatgga aatgacacag atgagtcatt ttcatcctaa tattcagtta
    12721 gaggaagtaa gccaacattt tgtacagtat tttttgactc aatttaaata ataggaaatt
    12781 atgaatttgt tagcagatta tgtgcaacca ctgacttatt ggctacaagc aaacccacat
    12841 tggtccctgt ttattacttt cttaatcgct ttaagcgaat ctttagctgt cattgggagt
    12901 attatacccg gctctgttac tatgactgca atagggatat tagcaggttc tggaatcatg
    12961 cgtattgatt tgacattact tgccgctact ctgggggcta tttgtggaga tagccttagt
    13021 tattttttgg gatatttcta tagtgatcgt ttaattgaga tttggccttt tagaaaatat
    13081 ccgggcttat tgaagtatgg taaagatttt tttgcaagcc atggcggtaa aagtgtatta
    13141 attggtcgtt ttgttggccc attgcgttct atcattccag ttattgctgg aataatgcat
    13201 atgaaacaat ggagattcct gatcgcaaat atagtttctg ctatcggatg gtctatttta
    13261 tatgttatgc caggggtgtt gattggtgcg gcaggacatg agttatcagc agagggagct
    13321 acaaggtttt ttatcttgat attggtcatt cttgctgtta tttggttgag tagcctcgtt
    13381 ctcaaatggt tatttattaa gttaaattca tttttgagaa ataacttgca tcgtttttgg
    13441 ttagaaatta agacaagccc cagattatct aaagtatatt atgctatcac tcctgttcag
    13501 gaggaaaatc attacaatac ggttggttta ttacttattg ctctcgtgat tttttttctc
    13561 tttttaactt tagttatttt taccattgaa ggtcaactac tagactatat taatgtgcct
    13621 gtgcattttt ttatgcaaag ctttcacagt tccttactgg aagcagtttt tattgtatgt
    13681 gctcaattaa caagtaaatc aactttagct gggttattta tcgcttcttg tatatggttt
    13741 atttatcata aaaattaccg agctacggtt tatttaagca gcataaccct attgagcggt
    13801 gttattgctt tatttttaac taagattatt gatagcccga gaccgcaagg actattggtt
    13861 actttgcctg gttcttcttt tccatctgtt agtatttcca tcgcaacagc actgtatggt
    13921 tttattttat tttacataaa tagtaagtat tccttaataa ccagcagttt taaaacagtt
    13981 attttgattg tgctcggact aagtgggttt gctaatatct atttaggtga tttttggttt
    14041 tctgatattt tagctgctta tctgttggga gctgaagtat gtttgattca ttgtttggta
    14101 tatagaaggt ttagtacttc tgctataaag aatgtacatc ctttaattat gctcatatca
    14161 ctgcttatgg ttattgtatt tgcaacagct gtttcaacag ttgtgaattt taaagaactg
    14221 gttcatgacc atatgcccta tcacaaggag tttactttaa cagaaaaaca atggtgggat
    14281 cagcaaagac ctcttttacc tttgtacagg ctaagccgaa ttggaaaacg aataagtctc
    14341 ttaaacattc aatatgtcgg tgatctggat gcattgcaaa ataagctaga acaatttggt
    14401 tgggaaccac agagcgataa aacttttttt accagattgc tgatgcgaat gaatagtgca
    14461 ccatcaaacg ttaaactgcc tttactcact caactatatg aaaataaaaa accaggcttg
    14521 ataatgacgt atcaagataa aaaatcaaag ttgatttttg atttgactat ttggcaatcg
    14581 aactattatt tatctgagcc taaaaggatg gtgtggatag gctccattca tcaaaatatt
//
LOCUS       DASLOE010000067        14591 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.067, whole genome shotgun sequence.
ACCESSION   DASLOE010000067 DASLOE010000000
VERSION     DASLOE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14591
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.067"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..523)
                     /locus_tag="U6309_001596"
     CDS             complement(<1..523)
                     /locus_tag="U6309_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEN5505037.1"
                     /translation="MDTTLDNPDYYINREFSAIAFNQRVLMLANDERVPLLERMRFLS
                     ICSSNLDEFFEIRVAGLKEKIAMSSGKLTIDGLRADEAFSQISHKAHALIDQLYNIFN
                     KQLLPALRKENIHFLETDQWTDDIHLWAKHYFKHEILPVISPIALDLAHPLPRLINKS
                     LNFIISLSGKDAFD"
     gene            complement(564..1769)
                     /locus_tag="U6309_001597"
     CDS             complement(564..1769)
                     /locus_tag="U6309_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN5505038.1"
                     /translation="MKRIFFVVFLLCLETACNTAKNNPLPAQLSDKALVPGFKEVRTE
                     FTSSDDPIIKKQSLERVKQLAKAYPDTMFHQKGSFSLLIISGGGDYGAFGAGLLNGWT
                     KSGSRPTFTTVTGISTGALIAPLAFAGPKYDATLKKVYTTITTKDVLSNKPVLWTLLT
                     GGSALKGTKPLQDLIARHVDEKLLDDIAKGYYQGRRLYVGTSNLYSRKLVVWDMTRIA
                     ASKNPERVQLFRKILLASASIPVVMPPVYFDVIANGKPYTEMHVDGSTTFAVFLSRLG
                     SDIKTAKKHFKLKQKPNVKIYVIRNNQNQFHYKVIKPKLLVIGKRAIESVTASQGAAD
                     VIFIYLCALIEGVDFNLANVPNEFDSNSEELFDTQRMNQLYQIGYSKAVKGYPWQKIP
                     PDVRALHGD"
     gene            complement(1845..2378)
                     /locus_tag="U6309_001598"
     CDS             complement(1845..2378)
                     /locus_tag="U6309_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEN5505039.1"
                     /translation="MIKTFIEIIYSFGKIGLISLGGGNSMLKLLEYEAVTYRHWIGKE
                     EFVGMLGSSFLFPGLTGIKLAALIGYKAAGITGLILAIICLNLPGLIMALVGYQWLNS
                     HNGPITRKIMISVQYGALALLAAATFSVVQGVIDVYYSIPMAICCILFFLALAFWNLS
                     PFYGFLAFIAVCFFVVR"
     gene            complement(2375..2995)
                     /locus_tag="U6309_001599"
     CDS             complement(2375..2995)
                     /locus_tag="U6309_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505040.1"
                     /translation="MQRLVWNFEFSSEQTTPLTTLGHDAQDNLKWEKRYFWPADQIIR
                     LNTIDSSLLDLANYQKKHKEDYYYLLPDSDHNIKKRRNELLYKPLIKQSGSALGFGPK
                     VHLENPQTLSCHGDAGQLNRQELLKKIKEKGIVVFVKKEAFVFKFPTTPTVKLELARL
                     EVKDKVYFSACIEGRSLNLVETISVLLFGTQPSFEYVTFLKKTLKI"
     gene            3171..5609
                     /locus_tag="U6309_001600"
     CDS             3171..5609
                     /locus_tag="U6309_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEN5505041.1"
                     /translation="MAIVFFLIYLAFTLVVLSRRINPLVWEIGSIIYLIPATFYFGFP
                     WIIGLFIWFFIIAAVLVVRVEPLRLFIGDYLYKTAGKSVPKLSKTEEEALNAGDTWLE
                     QDIFTGKPDWQRLSAVSTELTAEEQAFLDNETHTLCSMLDEWEISQEYDLSPKVWNYI
                     KEKGFLGLVIPKEFGGKGFSARAHSDIVMKIATRSRVAAVTVMVPNSLGPGELINYYG
                     TDEQKAYYLPRLAKGVEIPCFALTEPGAGSDATSIQSEAIVVKKKIDGKSVLGLNITL
                     DKRWITLAPVATLIGLAVNLKDPDGLLKGEGEEGITCLLIPRDTENLEIGNRHLPADQ
                     PFMNGTLRGKDIFVPIDTIIGGQKKAGSGWQMLVECLSIGRSISLPALGAGSSSISYL
                     TTGAFARIRRQFNVEIAQFEGVEEKLAEIAGLNYLINSTRLLTVAAVNEHKKPSVASA
                     ITKYFNTELARITVNASMDVHAGRAIVVGPRNYLNSLYQSVPISITVEGANIMSRNLL
                     IFGQGSMACHPFIRDEFYAISSENKEAFREIIWKHIHYFMQNFAKTVCSAWTSGLFIA
                     VPENSMKREYKKLARLSYAYSWLADLSLIYLGGDLKRKERLSARLADGMSYLYMAMAA
                     LRNVQLNQDHPDEQLHARWAVSYCFYHAQKSMIALCHNFPSKFLGFIARVVAFPFGQT
                     MRYPSDKLDHQLARLMTGNNHYRDRLKKSVFLSGDSKQPVDRMEHALQLIIEADGLSK
                     KVSDLKRYKWSKLKGKLKEKVESGQLSQQEMEELLAVEDARWDAILVDEFTFDSMKKK
                     TLHSVIEKMKSPLA"
     gene            5723..6415
                     /locus_tag="U6309_001601"
     CDS             5723..6415
                     /locus_tag="U6309_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0294 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505042.1"
                     /translation="MPKYTLPTRDALLKAMQVGETSIEAAEYMATRFEQILTKAKLLP
                     ECNDMLEKIKEYAQFVKFKLLSSAQVWSGQERPTSDYQNTQENKAEFLASHLEGLPSG
                     LKLEVAIGDDAKILRGFSSNGKMVEGDQLKTMDGLLEGWLAKNGLAISGGAVVKIDNA
                     GNQTKVDPQEIRQLINDSEKGVAKYFADKGVSMEVAQRTYQEPKALETKREEIRQEIE
                     SGAEAPTTQSIR"
     gene            complement(6452..7114)
                     /locus_tag="U6309_001602"
     CDS             complement(6452..7114)
                     /locus_tag="U6309_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEN5505043.1"
                     /translation="MKTAMILAAGRGERLRPLTDKIPKALCTVRNKPLIEHHIINLAN
                     AGFERLIINHAYLGGQIRQYIGNGKQWGLNVIYSPEPPGGLETGGGIVNALPLLGEEP
                     FLTVNADIYTDFDFAKLQLKNIDTFHLLLIPKNPSLLHHGDFGLINGSHLTNTNQAYT
                     FSGIACYNPKVFANCRQGRYSVTPLLRKYVEQNSATGSVHSGIWYDIGSFDRLQAANQ
                     FT"
     gene            complement(7111..8088)
                     /locus_tag="U6309_001603"
     CDS             complement(7111..8088)
                     /locus_tag="U6309_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEN5505044.1"
                     /translation="MHERENALKEWLAQTIQQKDFVLLPLAGDASFRRYFRIQYNGLT
                     QVVMDAPPEKENIEPFLHIANVLDKIKIPVPDILAINKREGFLLLSDLGDQLLLNKLN
                     HETVDNYYSQAMNLLLQIQKCPTDDHELPLFDKGFMLKEMNLCLEWFLKAYLSLDLKQ
                     DELQLFQHTIEWIASEVATQPRVFIHRDYHSRNLMLVKNKDSCLATIDFQDAMLGPVT
                     YDLVSLLKDCYISWPREKVLEWITYFHEKCPHANIYSLTDFIRAFDLCGLQRHLKVLG
                     VFCRLYLRDNKAGYLRDLPLTLKYALECAETYEELHPFFNFLQKRVYLP"
     gene            8331..10850
                     /locus_tag="U6309_001604"
     CDS             8331..10850
                     /locus_tag="U6309_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEN5505045.1"
                     /translation="MAIGITACVLSLINYQGLAYSAALINEPIQACVIAREVDLTDDI
                     RTKFAQCLGWQADQSSPVCLGFYKPITVTPLASPDEVRILADTASFYRTQRSTLSGHV
                     EMQQGQRVVNAQTAYVYRDPKTNEVTKIEFLNHVRYLEPDRMMIARKAVVYPQDKSGE
                     VEDVLYRFNTNRSNALLPAWGRASLIKRFANQDYFLKEATYTTCAPQDKAWAIEAESI
                     SIDNEKGKGIARNAKLRIHEWPVLYTPYLSFPTNRDRKSGFLMPIVGYSNVGGADLGI
                     PYYWNMAPNYDMTLVPHLYTKRGLMLGGQFRYLTSKSNGTFNGNFLPKDKAFGRFLQD
                     NEVEFPQIRGLSTNRWEVNFVDSTEFLSDLQLNVNFQQVSDDYYLQDFSTNLASVTQR
                     QLLRQADLTYTTENWTFRGMGQSYQTLHPINEIPVSPVYERLPQLMARGYYDDLPFNA
                     NFNILGQYDQFHWPNDSWNIALNNMPQGPRFHLNPILSVPMMKPWGYVTPSVQFVENY
                     YDISRNYTWGTSRANYNLTIPRYSLDGGLYFERDLHLKGTYYIQTLEPRLFYLRVPYY
                     NQTLIPVYDSGFMIFNVDQLFRTNRFSGFDRIGDANQLSYALTTRWLEDESGAEKANF
                     SIGQIKYFSERRVQLCQSPTGFCTDNPDTFGNLSSTFGTSPVASRAVYKFNPAWGITG
                     DYIWDPATRATNNADLNLHYQPARNAIINGGYSYLVNGDVTQVRNNDTENNALHQAIL
                     SAAWPLSEKWSGIGAYSYNISKNYSMMSFLGVQYDSCCWAMRVLGGRTFRSLNEEFEP
                     QYNNNIYLQILLKGLGSVASSDPSGILNTYIPGYYDPFRRR"
     gene            11026..12315
                     /locus_tag="U6309_001605"
     CDS             11026..12315
                     /locus_tag="U6309_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN5505046.1"
                     /translation="MFKRIALVCALFSGICFAEGKQLLDKVVAIVNDNVITSSELNAQ
                     VELSKKQIIAQNMQMPDESVLRKQVLQHLIDVDLEMQMAKQNGITIENAEIDEAIEKI
                     AASNHLNLSQMRDEITKQGISWQEYRQNIRKEMLISRVQQKAVGKDIIVTNEQVEQYL
                     KTSGRIENSNLTYHLKNIVIPLSEEPTTKQLQRAKIEAENLLNKIKKGEDFSRLAIEE
                     SSGEFALEGGDLGERHLAELPEVFAKEVVHMKVGQVAGPIRAGNGFHLIKLVAVGGEN
                     QRHVITQTHVRHILLKPDASMVPSEAIKQVNNIYRQIQSGKDFALMAKQYSLDAASAV
                     KGGDLGWVNPGELVPEFEKTMNSLPLHKVSKPVKTQYGWHLIEVIARRQKDDSEAFKK
                     QQVRQFLQQRKFVEAVQNWQQHLRSQAYINIVDKDLA"
     gene            12312..13286
                     /gene="pdxA"
                     /locus_tag="U6309_001606"
     CDS             12312..13286
                     /gene="pdxA"
                     /locus_tag="U6309_001606"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEN5505047.1"
                     /translation="MNPLLISSGEPAGIGPDLCLALAETDFPVVILGDLSLLEARASE
                     LNLSIKFLEYGPHQSFEKKAGYLTVWPVPCAAPVISGELNPQNAAYVMELLTLGASLC
                     SKGEFSALVTAPVHKANINAAGITFTGHTEFFADFFEVETVVMMLACSQMKVALVTTH
                     LPLRMVPDAISSLLIIKVIQQLHHSLKHDFGIQNPKINVAGLNPHAGESGYLGREEIE
                     IITPALNTLKNQGIDVLGPLPADTMFITNNINHCDAYVAMYHDQGLPVLKYAGFNEAV
                     NITLGLPIIRTSVDHGTALELAGKNKANPGSMLAAVKMAKDMALTRIK"
     gene            13288..13779
                     /locus_tag="U6309_001607"
     CDS             13288..13779
                     /locus_tag="U6309_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN5505048.1"
                     /translation="MSLISLIAAIDETGGLGINNQLLCYLPADLQHFKSITMGKPIIM
                     GRKTYESIGKPLPGRLNIVISQSIQSIPGVVVVDSLGQAIKETADASEVMLIGGAKIY
                     SQAISMANRLYITQIHHQFTADVFFPKINELVWSCRSKEHRQHDDKNQYDMTFCVYER
                     ISI"
     gene            complement(13786..14331)
                     /locus_tag="U6309_001608"
     CDS             complement(13786..14331)
                     /locus_tag="U6309_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3421 domain-containing protein"
                     /protein_id="HEN5505049.1"
                     /translation="MSKLVYLLLMIFFLNPAWANTHYHQHPVSPLANALRTGTDTNGN
                     ALYLCKAKLFNSIQPGKTWAGYGRCNVPYGGKEYVLSQFTIPNQNEFGRYSWEPNVEH
                     ALLMGKDTNGNPLFVCQSNFNGSIQPGKTWPGYSHCNISYGGREIITDNYRILSRPTE
                     IIVRTQPPVHRHPSQNRHYHY"
BASE COUNT         4536 a         2629 c         3023 g         4403 t
ORIGIN      
        1 gatcaaaagc atctttccca ctcaatgaaa tgataaaatt taaactttta ttgataagcc
       61 ttggaagagg atgggccaaa tccagagcaa tagggcttat aacaggtaga atttcgtgtt
      121 taaagtaatg ctttgcccat aaatgaatgt catcagtcca ttgatctgtc tcaagaaaat
      181 ggatattttc tttgcgcaat gccggaagta attgtttgtt aaaaatattg taaagctgat
      241 cgattaaagc atgagcctta tggcttattt ggctgaatgc ttcatcagcc cgtaatccat
      301 ctatggttag cttgcctgac gacatggcaa ttttttcttt caacccggct acccgaattt
      361 caaaaaattc atcaagatta ctgctgcaga tactgagaaa tctcattctt tccaataagg
      421 gtacgcgttc atcattagca agcatgagta ctctctgatt aaacgcaata gcagaaaatt
      481 ctctattaat ataataatct gggttatcca gagtagtatc catgaggaac tccaggtatt
      541 ttcatttcat gttcacaggc aatttagtca ccatgcaaag cccttacatc tggagggatt
      601 ttttgccagg ggtaaccttt taccgctttg gaataaccaa tttgatataa ctgattcatt
      661 ctttgagtat caaacaattc ctctgagttc gaatcaaact catttggaac attggctaaa
      721 ttaaaatcaa ctccctctat aagagcacaa agatagataa aaataacatc ggcagccccc
      781 tgggatgccg tgacactttc tatcgctcgc ttgccaatta ccaataactt gggcttgatt
      841 actttataat ggaattgatt ctggttattg cgtattacat aaattttaac gtttggtttt
      901 tgttttaatt tgaaatgttt tttcgcagtt ttgatatcag aaccaagacg acttaaaaag
      961 accgcaaacg ttgttgagcc atcgacgtgc atttctgtgt agggttttcc gttggctatg
     1021 acatcaaaat aaacaggtgg catgaccact ggaatgctgg cagaagcaag tagaattttt
     1081 cggaataact gaactctctc ggggtttttg cttgccgcaa tgcgggtcat atcccatact
     1141 accagctttc gagagtaaag attggatgtc cctacataaa gccgtcgtcc ttgataatag
     1201 cctttggcaa tgtcatcgag caatttttca tcgacatgac gagcaatcag gtcttgtaat
     1261 ggtttggttc ctttaagggc gctgcctcct gttaacagag tccataatac aggcttatta
     1321 ctcagtacat ccttcgtggt aatggttgtg tacacttttt ttaatgtagc atcatacttg
     1381 ggaccagcaa aagctaaagg tgctatcaat gctccagtac ttatacctgt cacggttgta
     1441 aatgttggcc gagaaccgga ttttgtccac ccattcagta atccagcacc aaaagcacca
     1501 taatcaccac cacctgaaat aatcagcaag ctgaaagagc ctttttgatg gaacatggta
     1561 tcagggtaag ctttagccag ttgttttaca cgttcaagag attgcttttt aattattggg
     1621 tcatcactgc tggtgaattc agtgcgtacc tctttaaatc cgggcactaa ggccttatct
     1681 gacaattgtg ctgggagagg attatttttt gctgtattgc aagcggtttc taaacaaagc
     1741 aaaaatacaa caaaaaaaat ccttttcata attttccttt aaattaatgt tttcctgaat
     1801 tttattcaaa aaatggatta aaagcgatag tgtttgtttt catattaacg caccacaaaa
     1861 aaacatacgg ctataaatgc taaaaaacca taaaagggtg atagattcca aaaggccaag
     1921 gcaagaaaaa acaaaataca gcaaatagcc ataggaatag agtaatagac gtcaataaca
     1981 ccttgaacta ctgaaaatgt agccgccgct aacaaggcca aagctccgta ttggactgag
     2041 atcataattt tgcgtgttat tggaccgtta tgagaattaa gccactgata tcccacaaga
     2101 gccataatta atcctggtaa attcaggcag atgatcgcaa gaattaaacc ggttattccg
     2161 gccgctttat agcctattag tgcggccagt tttattcccg ttaatcctgg gaataaaaaa
     2221 cttgaaccta acatacctac aaattcttct tttcctatcc agtgcctgta cgtgactgcc
     2281 tcatattcca ataattttaa catggagttg ccgccaccta aggatatcag gccaattttc
     2341 ccgaaactgt aaatgatttc aatgaaagtt tttatcatat ttttaaagtc ttttttaaga
     2401 aagtaacgta ttcaaaagaa ggttgagttc cgaataaaag gacgctgatg gtttctacca
     2461 ggtttaatga cctcccctca atacaggcac tgaaatacac tttatccttg acttcaagac
     2521 gagcaagctc aagcttgaca gttggcgttg tagggaattt aaaaacaaag gcttcttttt
     2581 taacaaaaac aacgattcct ttttctttta tttttttcaa tagttcttgt cgatttaatt
     2641 gcccagcatc gccatggcaa gagagcgttt gtgggttttc aagatgaact ttaggcccaa
     2701 aacctagtgc agagccagat tgcttaataa gaggtttata caataattca ttacgccttt
     2761 tttttatatt atgatcagag tcaggcagta aataataata gtcttcttta tgttttttct
     2821 gatagttggc tagatcaagc aacgaactgt caatagtatt taaacggatg atttgatctg
     2881 caggccaaaa atagcgcttt tcccatttta gattgtcttg agcatcatga cctaaagtgg
     2941 taagtggtgt tgtttgttca gaggaaaatt caaagttcca aaccagtctt tgcattatta
     3001 gtatttcatt ttctgttttg cttacgctag atgatttgat ttgttttggc aaggttaact
     3061 tcaatgtttc ttataatttt taaaggatta ttgctcgcta aataaagcgt aaaaagtata
     3121 tactggttat tctataaacc tcttaataaa atagcaaagg aaggtatttt atggctattg
     3181 tgttcttttt aatttatttg gcttttactc tggtagtact gtccagaaga attaatccat
     3241 tggtttggga aattggaagc ataatctacc tcattcccgc aacattttat tttgggttcc
     3301 cctggattat cggtttattc atttggttct ttattatagc tgccgtgctg gtagttcgag
     3361 tggagccatt acgccttttc atcggggatt atttgtataa aactgcagga aaatcggtac
     3421 ccaagctctc taaaacggaa gaagaagctt taaacgcggg ggatacctgg ctggaacaag
     3481 atatttttac aggaaaacct gattggcaaa gattatcagc agtttctaca gagctcacag
     3541 cagaagaaca agctttttta gataatgaaa ctcatacttt gtgcagtatg ttggatgaat
     3601 gggaaattag ccaggaatat gatttatctc ccaaggtttg gaattacatt aaagagaaag
     3661 gatttttagg attagtgata ccaaaggaat tcggtggcaa agggttctca gctcgagccc
     3721 attccgatat tgtaatgaaa attgcaactc gctcaagagt agcggcagtg acggtaatgg
     3781 ttcctaattc cctgggcccg ggtgagttaa taaattatta tggaactgat gagcaaaagg
     3841 catattatct tccacgtctt gccaaaggtg ttgagatccc ctgttttgca ttgacagagc
     3901 caggggctgg aagtgatgcg acctccatcc agtcagaagc cattgtagtt aaaaagaaga
     3961 tagatggaaa atcagtactt ggcttaaata tcaccttgga taaacggtgg attactttag
     4021 ctcctgttgc gactttaatt ggattggccg tgaacttgaa agatccggat gggcttttga
     4081 agggtgaagg cgaggaaggc ataacttgct tgctgattcc aagagatact gaaaatcttg
     4141 aaattggcaa taggcattta ccagctgatc agccctttat gaatggaact ttacgtggca
     4201 aagatatttt tgtgcctatt gataccatca ttggtggtca aaagaaagcg ggatccggtt
     4261 ggcaaatgct ggttgagtgc ttatctatag ggcgctcaat atctctgcca gcactcggag
     4321 caggttcttc ttctatttct tatttaacaa caggtgcttt tgccagaatt cgtcgccaat
     4381 ttaatgtaga aatcgctcag tttgaaggag tagaggaaaa acttgcagaa atagcgggtt
     4441 tgaattattt gatcaactct acccggttgt tgacggttgc tgcagtgaat gagcataaga
     4501 aaccctctgt agcttcagcg attaccaaat attttaatac ggaattggca cgaatcactg
     4561 tgaatgcttc tatggatgtt catgctggac gagccattgt tgttgggcca cgcaattact
     4621 taaatagttt atatcaaagt gttcctattt ctattacggt agaaggggct aatattatgt
     4681 ctcgcaattt attgattttt gggcagggat ctatggcctg tcatccgttt attcgtgatg
     4741 aattctacgc catctccagt gagaataaag aagcatttag agagataatt tggaagcata
     4801 tccattattt tatgcagaat tttgctaaaa cagtctgttc agcatggaca agtggtttgt
     4861 ttattgcggt tcccgaaaat tcaatgaaaa gagagtataa gaaactggcg cgtttgagtt
     4921 atgcctattc ctggcttgct gatctgtctt taatttattt gggaggtgat ttaaagcgca
     4981 aggagcgatt gtctgcaaga ttggccgatg gcatgtctta tctctatatg gcaatggctg
     5041 ctttacgaaa tgtgcaatta aatcaagacc atcctgatga gcaattgcat gccagatggg
     5101 cggtaagcta ttgtttttac cacgcgcaaa aatccatgat tgcactttgt cacaatttcc
     5161 cttccaaatt tttaggtttt attgcccgag tagttgcgtt tccatttggg caaaccatgc
     5221 gttatccttc agacaaacta gatcatcagt tggctcgttt gatgacgggc aacaatcatt
     5281 atcgtgaccg tttgaaaaaa tcggtttttc tcagtggtga ttcgaagcag cctgtggatc
     5341 gtatggaaca cgctttacag cttattattg aagcggatgg attaagcaaa aaagtgagtg
     5401 atttgaagcg ctataagtgg agtaagttga aagggaagtt aaaagaaaaa gtagagtctg
     5461 gccagttgtc acaacaggaa atggaagagt tgcttgctgt tgaagacgca cgctgggatg
     5521 ccattttagt ggatgaattt accttcgact ccatgaagaa gaagacattg cattccgtga
     5581 tagaaaaaat gaaatctccc ttggcgtgat ttttacagac ttttaacatc gatttgccaa
     5641 ccagctatca atccaaatga ggctggtttg ctataattaa actatatttc ctggaccaaa
     5701 tatacattgt tgagagtaag ttatgccaaa atacacactg cccacaagag atgccttatt
     5761 aaaagcgatg caagtaggtg aaacatccat tgaagctgct gaatatatgg caacaaggtt
     5821 tgagcagatt ttaacaaaag cgaagttatt accagaatgt aatgatatgc tggaaaaaat
     5881 aaaagaatat gctcaattcg ttaaattcaa attattatcc tctgctcaag tttggtctgg
     5941 ccaagaaagg ccaacttcag attaccaaaa cacgcaggaa aacaaagccg aatttttggc
     6001 aagccatttg gaagggttgc caagtggatt aaaactcgaa gttgctattg gggacgatgc
     6061 caaaatattg cgaggatttt cttccaatgg aaaaatggta gaaggagatc agctcaaaac
     6121 gatggatgga ttattagaag gatggcttgc caagaatggt cttgctattt caggaggtgc
     6181 ggttgttaaa atagacaatg cgggcaacca aaccaaagta gatccacagg aaatcagaca
     6241 attaatcaac gatagcgaaa agggagtcgc caaatatttt gcagataaag gcgtgagcat
     6301 ggaagtggca caacgaacct accaagaacc caaagcattg gaaaccaagc gagaagaaat
     6361 cagacaagaa attgaatccg gtgctgaagc cccaaccact caatccatca gataggaata
     6421 tactggttca cctaaaaacc aatcttggtt tttaggtgaa ctgatttgct gcctgcagtc
     6481 tatcgaaaga gcctatgtca taccaaattc ctgagtgaac acttccagtg gcactatttt
     6541 gttccacata ttttctgagt aaaggggtaa cagaatacct tccttgtctg cagttggcaa
     6601 aaacctttgg gttatagcag gcaatacctg aaaaagtgta agcctgattt gtattggtta
     6661 agtgggatcc attgattaaa ccaaaatcgc catgatgaag cagagaagga tttttgggaa
     6721 ttaaaagcag atgaaatgta tcaatatttt tgagttggag cttggcaaaa tcaaaatcag
     6781 tataaatgtc ggcatttact gttaaaaatg gctcctcgcc cagcaaaggc aaggcgttca
     6841 caatccctcc ccctgtttca aggcctcctg gcggttctgg agaatagata acattcaagc
     6901 cccattgttt tccatttcct atatattggc gaatttgtcc gcccaggtag gcatgattga
     6961 ttatcaatcg ttcaaagccg gcattggcca ggttaataat atggtgttca attaacggtt
     7021 tgtttcttac cgtacaaagt gctttaggaa ttttgtcggt taaagggcgt aatcgttctc
     7081 cacgtcctgc tgcaagaatc atagcggttt tcatggtaaa taaactcgtt tttgcagaaa
     7141 attaaaaaat gggtgtaatt cttcatatgt ttctgcgcat tctaatgcat attttaacgt
     7201 gagaggcaag tccctaagat atcctgcttt attatcacgc aaatataaac ggcaaaaaac
     7261 acctaacact tttaaatgcc tttgtaaccc acatagatca aaggcccgaa taaaatcggt
     7321 tagagaataa atattagcgt gggggcattt ttcatgaaaa taggttatcc attctaaaac
     7381 cttttctctc ggccatgaaa tataacaatc tttgagtaaa gaaaccaaat cataggtaac
     7441 aggacctagc atagcatcct gaaaatcaat agttgctaaa cagctatcct tatttttgac
     7501 tagcatcaga tttcttgaat ggtagtccct gtgaatgaaa actcttggtt gagtcgctac
     7561 ttcacttgcg atccactcga tggtatgctg aaatagttgt aattcgtctt gttttaaatc
     7621 aagagataga taggctttca aaaaccattc caaacataaa ttcatttctt tgagcatgaa
     7681 acccttatca aataagggaa gttcatgatc gtcagtggga catttttgaa tttgtaatag
     7741 caaattcatt gcctggctat aataattatc cactgtttca tgattcagct tatttaataa
     7801 taattgatcc cctaaatcgc ttagtaatag gaaaccttct cttttattta ttgccagaat
     7861 atcggggact ggtattttaa ttttatcaag aacgttagcg atatggagga aaggttcaat
     7921 attttccttt tcaggtggag cgtccataac tacttgagtt aatccattat attgaatgcg
     7981 aaaatacctt ctaaaacttg catctcctgc taaaggaagg aggacaaaat ctttttgttg
     8041 aatcgtttga gctaaccact ctttcagtgc gttttctctt tcatgcatgg tatacttccc
     8101 gtttttattt acagagtgcg gttgatatac tcccttctta ataaagagta atttcattcg
     8161 cgcacaaata ataaggtatt ttgttagctg tgaacatgag gttttataaa aagctaatta
     8221 tatccggttt tatttcattt atcgccttat tgcgaggttt actctattgc catttaaaaa
     8281 ggttatttaa aaaatggcct gaatctttat tttcagtgaa atgggtctta atggccatag
     8341 ggataactgc ttgtgtttta tctttgataa attatcaagg gttagcctac tccgctgcct
     8401 tgataaatga acctattcaa gcttgcgtga ttgcgcgaga agtcgattta acagacgata
     8461 taagaactaa atttgcccaa tgtttgggtt ggcaagctga tcaaagttcc cctgtgtgcc
     8521 tcggttttta caaacccatt acggtgacac cgttagctag tcctgatgaa gtacgtattc
     8581 tggcagatac ggcttccttc tatcgtactc aaagatccac tttatctggg catgtagaaa
     8641 tgcagcaggg ccaaagagta gttaacgctc aaacagctta tgtgtatagg gatcctaaaa
     8701 cgaatgaagt aacaaaaatt gaatttttga atcatgtacg ttatttagaa cctgatagaa
     8761 tgatgatcgc tagaaaagca gttgtgtatc ctcaagataa atcgggtgaa gtggaagatg
     8821 ttctttatcg atttaatacc aatagaagca atgcattgct acctgcctgg ggtagggcaa
     8881 gtttaatcaa gcgttttgca aatcaggact actttttgaa agaggcaact tacacgactt
     8941 gcgcgcctca agataaggct tgggctatag aggcggaatc catcagcata gacaacgaaa
     9001 aaggaaaggg cattgctcgc aatgctaaat tacgtatcca cgaatggccg gtgctttata
     9061 ccccctattt aagtttccct accaatagag atagaaaatc ggggtttctg atgcccatag
     9121 tgggttattc caatgtaggg ggagctgatt tgggcatccc ttattattgg aatatggcgc
     9181 caaattatga catgactctc gttcctcacc tttatacaaa acgcggcttg atgctgggag
     9241 ggcaatttag atatttgact tcaaagagta atggaacatt taatggaaat tttttaccaa
     9301 aagataaagc ctttggcaga tttttacagg acaatgaggt agagttccct cagattcgag
     9361 gactttccac gaatcgttgg gaagtaaatt ttgtcgattc aaccgaattt ctctctgatc
     9421 ttcaattaaa tgttaatttt caacaggttt ctgatgatta ttacttgcaa gatttcagta
     9481 caaatttggc atcggtaact caaagacaat tattgcgcca ggctgattta acctacacta
     9541 ccgaaaactg gacttttaga ggtatgggac aaagttatca aacgttacat ccaattaacg
     9601 aaatccctgt ttcgcctgtt tatgaacggt taccgcaact catggcacga ggttattatg
     9661 atgatttacc ttttaatgcc aatttcaaca tccttggcca atacgaccag tttcactggc
     9721 ctaatgactc ctggaatata gcacttaata atatgcctca ggggcctcgg tttcatctga
     9781 atcccatttt atcagtgcca atgatgaagc cttggggata tgttactccc tcagtacaat
     9841 ttgtggaaaa ttattacgat atttcaagaa attatacttg gggtacatct cgagctaatt
     9901 ataacctgac aatacctcgc tatagccttg atggcggatt gtatttcgaa cgagatttgc
     9961 atcttaaggg tacgtattac atacaaactt tagagcccag gttattctat ttaagagtac
    10021 catattataa ccaaacactt attccagttt acgattcagg atttatgatt tttaatgtcg
    10081 atcaattgtt tagaaccaat cgcttttcag ggtttgatag aattggagat gccaatcagc
    10141 taagttatgc ccttaccaca aggtggttag aagatgaatc aggtgctgag aaagcgaatt
    10201 tttctattgg tcaaatcaaa tatttcagtg agagaagagt acaactttgt caaagcccta
    10261 ccgggttttg taccgataat ccagatacct ttggaaactt atcttctact tttggaacat
    10321 cgcctgttgc gtcaagggcc gtatacaaat ttaatccagc ttggggaata acaggggatt
    10381 acatttggga tccggcaaca agggctacaa ataatgcgga tttgaatttg cactatcagc
    10441 cagctcgtaa tgctatcatc aatggcgggt atagttattt agttaatggt gacgtcactc
    10501 aagtaagaaa taatgacact gaaaataatg ccttacatca agccatttta tccgctgcct
    10561 ggccgcttag tgaaaaatgg agtggtattg gagcctatag ttataatatc agtaaaaatt
    10621 acagtatgat gtcatttctt ggcgttcaat atgatagttg ttgctgggct atgcgtgttt
    10681 tgggagggcg aacttttaga agtttaaatg aagaatttga gcctcagtac aataacaaca
    10741 tttatttgca aatattacta aaaggcttag gatcagtagc tagcagtgat ccaagtggta
    10801 tattgaatac ctatatccca ggatactatg accctttccg acgccgttaa aatccaaaga
    10861 tcaatgacgc aataaaagcg agtcaggttt aggtgtttag gttagtacag gttgaaaata
    10921 ttttaatctt ttttttatga gttatggtgt aaattaagca aacttgcttg tattttgaca
    10981 aagaataaca taaattgccc gaaaaatgta aatgaggatt atggcatgtt taaaagaata
    11041 gcattggtat gcgctttgtt ttcaggaata tgtttcgctg aaggaaagca gctgttagat
    11101 aaagtggttg ctatagtaaa tgataatgtc attacttcca gcgaattgaa cgctcaagtc
    11161 gagttatcta aaaagcagat tatcgctcaa aatatgcaaa tgccagatga gtctgtatta
    11221 cgtaagcaag tattgcaaca tttaatagac gtagatctgg aaatgcaaat ggcaaaacaa
    11281 aatggtatta ctattgaaaa tgctgaaatt gatgaagcga tagaaaaaat agctgcttca
    11341 aatcatctaa atttatcgca aatgcgcgat gaaattacaa agcaaggaat tagctggcaa
    11401 gaatacagac aaaatatccg taaagaaatg ctgatttcca gggttcagca aaaagcagta
    11461 ggtaaagata tcattgttac taatgagcag gtggaacaat acttaaaaac ttcaggccgc
    11521 atagaaaatt ctaacttgac ttatcattta aaaaatattg ttatcccttt aagtgaagaa
    11581 ccaacgacca aacagcttca aagagctaaa atcgaagcag aaaacttgtt aaataaaatt
    11641 aaaaaaggtg aagactttag ccgtttggca atagaagaat cgagtggaga gtttgcactg
    11701 gaaggtggtg atttgggtga gcgtcatttg gcggagctgc ctgaggtatt tgccaaggaa
    11761 gtagtacata tgaaagtagg tcaggtggcc ggcccgataa gagcaggaaa tggatttcat
    11821 ttgatcaaat tagtagctgt tggcggcgag aatcagcgtc atgtgatcac tcaaacccat
    11881 gtcagacata tactgcttaa gcccgatgcc agcatggtgc catctgaagc aattaagcaa
    11941 gttaataaca tttaccggca aatacagtca ggcaaagatt ttgctcttat ggctaaacaa
    12001 tattcactcg atgctgccag cgcagtgaaa ggcggtgatt taggatgggt taatcctggt
    12061 gaattagttc cagagtttga aaaaaccatg aacagtttgc cattacataa agtcagtaaa
    12121 ccggtaaaaa cccagtacgg gtggcatctc attgaagtga ttgcacgcag acagaaggat
    12181 gattcagaag cttttaagaa gcaacaggtc aggcaatttc tacaacagcg taagtttgta
    12241 gaggcagtgc aaaactggca acaacatttg cgttctcaag cttatattaa tatagttgat
    12301 aaggatttag catgaaccca ctgcttatta gtagtggaga gcctgctgga attggcccgg
    12361 acttatgcct ggctttggca gaaactgatt ttcccgtagt gatattgggt gatctgtctt
    12421 tattagaggc aagagccagt gagttaaatc tcagtattaa atttttagag tacggccctc
    12481 atcaaagctt cgaaaaaaaa gcaggatatt taactgtatg gcctgttcca tgcgctgcac
    12541 cggtcattag tggtgaatta aatccgcaaa atgcggctta tgttatggag cttttgactt
    12601 taggggcttc gctatgctcc aaaggtgagt tttccgcatt agttacagcg cctgttcata
    12661 aggcaaacat taatgcagcc ggtattacgt ttactggcca cacggagttt tttgctgatt
    12721 tctttgaggt ggaaacagtg gtgatgatgt tggcctgttc tcaaatgaaa gtggcattag
    12781 tcacaacgca tcttcctctg cgtatggtgc ctgatgccat tagctctttg cttatcataa
    12841 aggtaattca acaattacat cattcattaa aacatgattt tggaattcaa aatccaaaaa
    12901 tcaacgtggc tggtctgaat ccgcatgctg gagaatcagg atatttaggt cgagaagaaa
    12961 ttgaaattat tactccagcc ttaaatactc taaaaaacca ggggatcgat gtactagggc
    13021 cgttaccagc agatacgatg tttataacaa acaatatcaa tcactgtgat gcttatgtgg
    13081 caatgtacca tgatcaagga ctgccagtct taaagtatgc tggatttaat gaagcagtaa
    13141 atataactct cggattgccg ataattcgta cttctgtaga tcatggaaca gcattagaat
    13201 tagctggtaa gaacaaagct aaccctggga gtatgcttgc ggcggttaag atggcgaaag
    13261 atatggcttt aacaaggata aaatgaaatg tctctgatta gcctgatagc agccatagat
    13321 gaaacaggag gattgggtat taacaaccaa ttactctgct atttgccagc cgacttacag
    13381 cactttaaat cgattaccat gggtaagcct attatcatgg gcagaaaaac ttatgaatcg
    13441 ataggcaaac ctcttccagg cagactaaat attgtgatta gtcaatcgat tcagtcaata
    13501 ccgggtgttg ttgttgtcga ttctttgggc caggccataa aagaaaccgc tgacgcatca
    13561 gaggtaatgc tcattggagg agccaagatc tattctcaag ccatatcaat ggctaatcgc
    13621 ttatatatca ctcaaataca ccatcaattt acggccgatg tgttttttcc aaaaatcaat
    13681 gaattggtat ggagctgccg aagcaaggag catcgacagc atgatgacaa aaatcaatac
    13741 gatatgacat tttgcgtata tgaaagaata tcaatttgat agctatcaat aatgataatg
    13801 tctgttttgt gagggatgtc tgtgcactgg tggctgagtc cttacaatta tttcagttgg
    13861 tctgctcagt attctataat tgtccgttat aatttcccga ccaccataag aaatattaca
    13921 atgtgagtaa ccaggccatg ttttccctgg ctgaatactt ccattgaagt tactttgaca
    13981 gacaaataaa ggattaccat tggtatcttt acccattaac aatgcatgct ctacgttggg
    14041 ttcccagctg tatcgcccaa attcattttg attcggtatg gtaaactggc taagaacgta
    14101 ttctttacca ccataaggta cgttgcatcg accatatcct gcccatgttt ttccgggctg
    14161 tatactgtta aacagttttg ccttacacag atacaatgca ttgccattcg tgtcagttcc
    14221 agttcgtaac gcattagcta acggtgaaac aggatgttga tggtaatgag tattggccca
    14281 ggcaggatta agaaagaata tcatcaataa aaggtataca agtttggaca tatcaactct
    14341 ctaaaaattt aaagagccgc aagcataacg caagagaaaa atatgcgcaa gatgaatact
    14401 taaagatcga tatggtttat ctattgtaac ctaaagaaaa acaattaaaa aaaattaaaa
    14461 aagtatttga cagtggtgat gaaatgagta gaatacgccc tacgccacga gggcgggttc
    14521 attaagaaga gagaagacaa actgtgtggg cactttgaga tactttgagt gctttagaga
    14581 gataaagaaa t
//
LOCUS       DASLOE010000068        14448 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.068, whole genome shotgun sequence.
ACCESSION   DASLOE010000068 DASLOE010000000
VERSION     DASLOE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14448
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.068"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..676)
                     /gene="ccmB"
                     /locus_tag="U6309_001609"
     CDS             complement(<1..676)
                     /gene="ccmB"
                     /locus_tag="U6309_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEN5505050.1"
                     /translation="MNSPFQLFGKQFRREILIQVRQIRYLVNSCLFFLMLLFIFPLTL
                     RPELMLMRMFAPGLVWMAILLSMLLSLERLFQQDYEQGVLEQWLVSGQPLNLLVTAKV
                     MAHWLISVTPLLLLSPLIAILFSFNVWETCILLLSLICGTPAILFLCALAAAFGIGIN
                     QRGALMALILLPLTLPLLIFGSGTLSVAMQGLPVSGYLALLLAMSLLAVGFLPYAIAA
                     VIRISHI"
     gene            complement(673..1275)
                     /gene="ccmA"
                     /locus_tag="U6309_001610"
     CDS             complement(673..1275)
                     /gene="ccmA"
                     /locus_tag="U6309_001610"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027225196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEN5505051.1"
                     /translation="MLDVIELDFDYHDQPLLQQISFHLPAGGLLHLKGSNGAGKTTLL
                     KLIAGLLNPEKGEILFERRSIKKDLCTYQKQLCFVGHRSGINPYLTLRENCLYDIHFS
                     PGAVGITELCRLFSLEHLIDYPCGLLSSGQKRQVALLRLWMSKAKLWLLDEPLVALDE
                     LSLLTIIRKIQEHRAKGGAVLLTSHQDLPLNKADYEEYHL"
     gene            1423..2358
                     /gene="sohB"
                     /locus_tag="U6309_001611"
     CDS             1423..2358
                     /gene="sohB"
                     /locus_tag="U6309_001611"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEN5505052.1"
                     /translation="MEFLSEYGMFLLKCITLVIALLILLAGIFSMGRKTKPKLEITSL
                     NEEYQHLNALMNKEILGKKPGKKKKDKTKRPVLYVIDFSGDIKATQVEQLRDEVTSVL
                     SIAKPEDEVLVRLESPGGAVNGYGLAASQLQRIRDKKIPLTVSIDKMAASGGYLMACV
                     ANKIIAAPFAIIGSIGVVAQIPNFHRWLKKNNIDVELLTAGEYKRTLTLFAENTEKGR
                     KKFQEDLEKIHTAFREYVLKNRSQLDIDKVATGEHWIAKDAFDLRLVDKLATSDEYLI
                     EKMAEFNAFKLTVHAKLPIISKVLKPAMRLIHPWI"
     gene            2581..2862
                     /locus_tag="U6309_001612"
     CDS             2581..2862
                     /locus_tag="U6309_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505053.1"
                     /translation="MSDFKSKLPDLNELTSMTAKLFKGIKTSVSEIIQDYKQKRAQPV
                     STEEKTTENKPAESKAEPKAERKTTENKTEPKTQSKPESNTEETQEKEK"
     gene            complement(2906..4321)
                     /locus_tag="U6309_001613"
     CDS             complement(2906..4321)
                     /locus_tag="U6309_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEN5505054.1"
                     /translation="MRSNDRIYIIDDDKERCDKLRTIFDFIGQFSEVTQYGNWQLSVN
                     PNPSVIVLGACTSFEKMMDELDTLVKKFPKTPILVIDAVLSTSECADRNIVDCLSFPF
                     SYAKMLKSLHRCQIAKEAVKFITAGTHKTPLFRSLVGNSEGIRQVRKLIEQVADTEAS
                     VLILGESGTGKEVVARNIHSLSSRANKPFVPINCGAIPGELLESELFGHEKGAFTGAI
                     TSRQGRFELANGGTLFLDEIGDMPLAMQVKLLRVLQERCFERVGSNKSIEVNVRIIAA
                     THRNLEIAIQEGKFREDLFYRLNVFPIEMPPLRNRSSDLSLLFNELVSRIEGEGRPTV
                     RLMPDAMDALCEYSWPGNVRELANLVERLSILYPNGIIGKSDLPHRFQGENKPSCPEA
                     SGAERETLLQIIKQESVANAEGIDLKEHLVKTELALISQALDESDWVVAHAASYLNMR
                     RTTLVEKMRKYGLTRPERMER"
     gene            complement(4517..5110)
                     /locus_tag="U6309_001614"
     CDS             complement(4517..5110)
                     /locus_tag="U6309_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505055.1"
                     /translation="MSNFRNFLVFILLFFSFCGQLTASSKQNLAFRNFWHPTYLGQRL
                     DYCTLDGKECGKEVANRYCQMLGYDYSTQNVIAYNVGLTNYLGSKAQCKGWRCNGFMT
                     IACAIGLSHTPPKPYHYREKKFAVPRYNDYRVDWCMERNKGCGKQAANSFCNRMGYMQ
                     AKDFVKQAQVSATKTIGSQELCFGNQCNAFKMIICYR"
     gene            5499..6515
                     /locus_tag="U6309_001615"
     CDS             5499..6515
                     /locus_tag="U6309_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEN5505056.1"
                     /translation="MKKYLTFIVSGLALIAIIWFIRHLWIIGTVKVVHPVYGTAVQAV
                     YATGTVEPTVMMPIASQNTARLVKLNADEGSEVVKGQILASLEDEHLQHNLKELKEHE
                     KLAKIRYERNAQLVKKGVISKDEYDRTLSEWQAASAAVRRIEAQISYLQLIAPADGRI
                     IKRDGEVGQLIPANQPVFWLSCCAPLRVSAEVDEEDIVLVRVGQKVLIRSDAFPNQIF
                     YGRVKSITPKGDPIARSYRVRISFSENNPLLIGMTAETNIIVHEKKNALLLPVSSISE
                     NKVWIVQDDGKLSQNTVTIGARGLKQAEILKGVNINDLVVLQPDNKLKNGQKVRFILV
                     KQEE"
     gene            6515..7765
                     /locus_tag="U6309_001616"
     CDS             6515..7765
                     /locus_tag="U6309_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN5505057.1"
                     /translation="MKLFLKIALKHLLARKRQSLVSLMGIVLGVAFFLTISSLMQGSE
                     KDFIKRLVDNSPHITIIDEYRNPRIQPIQQLHTHGAIEIRSVKPLTEPRGIRGYEQTI
                     NNLSLTIPGIVISPVLIGQALISYAGKDYSVTLNGMIPEEIKKVSTIENYMTSGSIDD
                     LIVNPDGIVLGSELLRKLSLTKDDNITVTAPNGQIRTFKILGVFRTGRSDFDTNQAFI
                     SIKRAQALLNRPNRSNNIIIKLPNPYQAYDIAAQIEKQIGYKTISWQEKSEDLLNTLV
                     IRNIIMYSVVSAVLIVAAFGIYNVISTVVMEKHRDIAILKSIGFQKHDIQFIFIIEGF
                     LLGLVGCLLGLPLGSGLMYSLMQVQFKPPGSSELINMPLDWSYLQFVIATAFAMSASI
                     IAAVLPAHKAALVHPVDILRGGGM"
     gene            7762..8439
                     /locus_tag="U6309_001617"
     CDS             7762..8439
                     /locus_tag="U6309_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN5505058.1"
                     /translation="MNKIVIKTKELSRQLSGEVPVTLVKDINLEVREGEFLVISGPSG
                     SGKSSLLYLLGLLDRPSQGTIWLNNEDVSTYEEEKMAWLRLTQIGYVFQFHFLLPEFT
                     ALENVMLPMQRLGKLSLGEMKNRATKLLTSLGLEEQLSKLPKQLSGGQSQRVAIARAL
                     ANDPILLLADEPTGNLDTVSSTNVQNILKELAHEYRRSVIIVTHDMNFAATADRVIHI
                     VDGKMQE"
     gene            complement(8625..9383)
                     /locus_tag="U6309_001618"
     CDS             complement(8625..9383)
                     /locus_tag="U6309_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEN5505059.1"
                     /translation="MITRDELSLFLLDFLNCSQYQDYAPNGIQVEGKDKIKRICTAVS
                     ASEDVISQAIEQQADALLVHHGYFWKGENPVISGMKRRRIAKLLGHNMNLFAYHLPLD
                     CHPELGNNASLANLLLIESHEMHKVNNTPNLLWSGKLSKAMNSEQFSSFLEHKLGRYP
                     VHIAGNEKMIHSIAWCTGAAQDFIEEAYQLGVDAYLSGEVSERTFYQAMELGIQYFSC
                     GHHATERFGIQSLGVYLANYFDLEHWFIDSPNPI"
     gene            complement(9380..10648)
                     /gene="murA"
                     /locus_tag="U6309_001619"
     CDS             complement(9380..10648)
                     /gene="murA"
                     /locus_tag="U6309_001619"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN5505060.1"
                     /translation="MDKLLINGGKALHGEVVISGAKNAALPIMAASLLASDHVTISNV
                     PHLKDITTMMELLGQLGAHLIVDEKMNVQVDSSQVNEFVAPYDLVKTMRASILVLGPM
                     LARFGKADVSLPGGCAIGTRPVDLHLKALRAMGADITVKNGYINARCKKGRLQGKRLM
                     FDTVTVTGTENVLMAAVLAEGITTIKNAAREPEVVDLANFLIQMGAKIRGAGTSTIEV
                     EGVESLNGGTYSVMSDRIEAGTYLAAGALTRGQVTVKKVRPDTLLSQLCKFEEAGAEL
                     TIGEDWVSLNMHNKRPQAVNISTAPYPAFATDMQAQFMAMNSVAEGSSTIIETIFENR
                     FMHVQELQRMGANIQLNGNTAIVHGVEKLTGAPVMATDLRASASLILAGLVAEGETVV
                     ERIYHVDRGYERIEEKLSLLGADIKRVSDR"
     gene            complement(10641..10886)
                     /locus_tag="U6309_001620"
     CDS             complement(10641..10886)
                     /locus_tag="U6309_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEN5505061.1"
                     /translation="MLSNEEIEQRLNEIDDVFYVKVEGDGYQYQVTVVTDIFLKKSKV
                     ARQQWVYAQLKDFITTGRLHAISMKTWTKEEWGKQNG"
     gene            complement(11276..11557)
                     /locus_tag="U6309_001621"
     CDS             complement(11276..11557)
                     /locus_tag="U6309_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEN5505062.1"
                     /translation="MEINNFKPGAELTFKSVVSVRDKLYKALMEDARERFCLDLSEVT
                     HCDSAGLALIIEARKLCKQNNKVFDVIGISPETQSLAEFCGVKTILQRS"
     gene            complement(11560..12168)
                     /locus_tag="U6309_001622"
     CDS             complement(11560..12168)
                     /locus_tag="U6309_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN5505063.1"
                     /translation="MRVFKTILLVVLMTLSQAMVAQTSPIPMLERAANQIISILKENK
                     ARLKSNPEIIYKAVEENLLPNVDVAGMSRSVLGRQAWSKATEAERVQFSKAFTRLVIR
                     TYSSPLAQYSDETVQFLPLRGSLNSRFIRVNSVIVRSAGQNIPLSYSLVSKNGKWKIY
                     DISVEGVSLLQSFRSQFAQALQHSSISQVIKEMQQKQIKKAS"
     gene            complement(12176..12652)
                     /gene="mlaD"
                     /locus_tag="U6309_001623"
     CDS             complement(12176..12652)
                     /gene="mlaD"
                     /locus_tag="U6309_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEN5505064.1"
                     /translation="MKKQRYVDISVGIFMLLGLLALLVMAMKVSNITDFMSDKTYRVT
                     ADFTDIGGLKVRAPVTVAGVRIGEVSRIELQPGELNARVTMTLNSDKKIPYEDASARI
                     LTEGLLGSNYISIVPGFDEDGDTDHPYLRDGDTIAKTQEAIILENLIGQLLFNIKK"
     gene            complement(12653..13435)
                     /gene="mlaE"
                     /locus_tag="U6309_001624"
     CDS             complement(12653..13435)
                     /gene="mlaE"
                     /locus_tag="U6309_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEN5505065.1"
                     /translation="MLDTIARLGNRGVQALQGIGSSGLFLFSMLFRKPNVLRLWPLLR
                     YQLYFIGVLSCLIIVVSALFIGMVVGLQGYNTLQKFGASSQLGQLLALSVARELGPVI
                     SALLFAGRAGSALTAEIGLMKATEQLSSMDMMGVDPLGRVVYPRFVAGIIALPILALI
                     FSAVAIYGGYFIGVYWLGVDAGSFWSNMQAAVNFRIDILSGIIKSLVFAFVVTWISVY
                     QGFECVPTAEGISHATTKTVVYSSLAVLGLDFLLTAMMIGDW"
     gene            complement(13428..14225)
                     /locus_tag="U6309_001625"
     CDS             complement(13428..14225)
                     /locus_tag="U6309_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN5505066.1"
                     /translation="MPDNWVEIKNLFFSLDDRCIFDGIDMVVKQGKITAIMGPSGSGK
                     TTLLRLIGAQLRPDSGQIYVNGQNIHQLSRKSLYETRRNMGLLFQSSALFTHLSVFEN
                     VAFPLREHTELNASMIRNIVLMKLEAVGLRGAAHLMPAQLSGGMCRRVALARTIALDP
                     VLMMYDEPFTGQDPISMGVLVRLIQRLNQLLHTTTIIVSHDVEETCSIADYIYLISGG
                     KIIGQGTPDELKRSIEPQIRQFMHGEADGVVPFHYPAKPYTEELLNA"
BASE COUNT         4744 a         3022 c         2501 g         4181 t
ORIGIN      
        1 ctatatgact aatacgtatc actgccgcaa tcgcataggg tagaaaacca actgccaata
       61 gagacatcgc caataataaa gccagatagc cgctgaccgg caaaccttgc atcgcaacgc
      121 tcaaagtacc actaccaaat atcagtaaag gtaaagttaa cggcaataga atcaatgcca
      181 tcaaagcccc gcgctgattt atccctatac caaaagctgc tgcaagcgca cataaaaata
      241 atatggcagg cgtcccgcaa ataaggctca ataacaatat acatgtttcc caaacattga
      301 atgaaaataa tattgcaatc aaaggagata ataataatag cggtgtcaca gatatcaacc
      361 agtgtgccat aactttggct gtcacaagga gatttaatgg ttgccctgaa actagccact
      421 gttcaagaac cccttgttca taatcttgtt gaaacaaccg ttctaacgaa agaagcatgg
      481 ataataaaat ggccatccac actaatccgg gagcaaacat acgcatcaac atcaattctg
      541 gtcttagagt aagaggaaaa ataaataaca acattaaaaa aaataaacac gaattaacta
      601 aatatctgat ttgccgcacc tggattaata tttcacgcct gaactgtttt ccgaataatt
      661 gaaatgggga attcatagat gatattcctc ataatctgcc ttgtttaaag gcaaatcctg
      721 atgagaagtc aataatacag caccaccttt tgccctgtgc tcctggattt ttctgataat
      781 cgtaagcaat gataactcat caagagcaac caaaggttca tcaagcaacc ataattttgc
      841 ttttgacatc cataatctta ataaagcaac ttgccttttt tgtcctgagg atagtaatcc
      901 acatggataa tcaataaggt gttctaaact gaataatctg cataattctg taatgcccac
      961 tgcaccagga gaaaaatgta tgtcatacaa acaattttct cttagcgtaa gatagggatt
     1021 gatccctgat ctatgcccca caaaacataa ttgcttttga taggtgcata gatctttttt
     1081 tatagatcgc ctttcgaaga gaatttctcc cttttcagga ttaagcaaac ctgctattaa
     1141 tttaagtaaa gtcgtttttc ctgctccatt ggaacctttt aaatgcaata agcctccagc
     1201 aggaagatga aaagaaattt gttgcaacaa tggctggtca tgatagtcaa aatcaagttc
     1261 aatcacatca agcataaaac actttatttt tagatacact cggctaacta taacaggaca
     1321 taagaaaaat ccccaatgcc ttcgttaaaa gttatttaat ttttgcttgc gaagtgagtg
     1381 gatgatcgcc ataatctgct ttattttttg caaaggtata tgatggaatt ccttagtgaa
     1441 tatggcatgt ttctcttaaa atgcatcact ctcgtcatcg cattactcat tcttctggca
     1501 ggtatatttt ctatgggtcg caaaaccaaa cccaaactgg aaattacctc tcttaatgaa
     1561 gaatatcagc acctgaatgc cttaatgaat aaagagattt taggaaaaaa acctggaaaa
     1621 aagaaaaaag ataaaaccaa gcgcccggtt ctttatgtca ttgatttcag cggtgatatc
     1681 aaagcaaccc aagttgaaca actaagagat gaagtcacat cggtattaag tatagcaaaa
     1741 cctgaggatg aggttttagt aaggttggaa agccctggag gggccgtcaa tggttatgga
     1801 cttgctgcgt cccagttaca gcgaatccgt gataaaaaaa ttcctttgac tgtcagcata
     1861 gacaaaatgg cagccagcgg cggatattta atggcctgcg tagcaaataa aataattgct
     1921 gccccatttg caataatcgg ctctataggc gtagttgctc aaattcctaa ttttcatcgc
     1981 tggttaaaga aaaacaacat tgatgttgag ctattaactg caggagaata caaacgcacc
     2041 ctaacacttt ttgcggaaaa tactgaaaaa ggtagaaaaa agtttcagga agatttggaa
     2101 aaaattcata ctgcattcag agagtatgtt cttaaaaata gaagtcaact cgatatagat
     2161 aaggttgcaa ctggtgaaca ttggatagca aaagatgctt ttgatctccg gctcgttgat
     2221 aagttagcta ctagcgatga atatttaata gaaaaaatgg cggagtttaa tgcattcaaa
     2281 ttaactgtcc atgccaaatt gccaattata agcaaggtgc ttaaaccggc aatgagactc
     2341 attcatccgt ggatttaaaa attagtactg aaacagcttg ggaacgccat tgaaattcaa
     2401 taccgttaaa tcaggcagca ttccatagga aacgagatca attttatcat gggcttaaag
     2461 ctgatgatgg tagcggaatt taaggaatta atgagcaaaa attttcaaaa tccgccatta
     2521 accaaaattt gaatctcggt atataattaa agaataacaa ttttttctga ggagtaagac
     2581 atgagtgatt tcaaatcgaa gttaccagat cttaatgaat taacatcaat gaccgcgaaa
     2641 ttatttaaag ggattaaaac cagtgtcagt gaaataattc aggactataa gcaaaaaaga
     2701 gcacagcctg ttagtactga agagaaaacc acagaaaata agccagctga aagcaaggcc
     2761 gaaccaaaag cagaaagaaa gaccactgaa aacaaaactg agccaaaaac acaaagcaaa
     2821 cctgaatcaa atacagaaga aacacaggaa aaagagaaat aatcctgtgc tagcaaataa
     2881 gtgatacggg gtgagctgta tctatctatc gttccattct ttctggtcga gttaagccat
     2941 atttgcgcat tttttcaact aaagttgtcc ttctcatgtt gagataactg gccgcatgag
     3001 caacaaccca atctgactca tctaaggcct ggctaatcaa ggcaagttct gttttaacca
     3061 aatgctcttt taaatcaatg ccctctgcat tagctactga ctcctgcttt attatttgca
     3121 acaaagtttc acgttccgcc cccgatgcct ctggacaaga tggtttattt tcaccttgaa
     3181 accgatgagg taaatcactc ttgccaataa taccattagg gtaaagaata gataatctct
     3241 ccaccaaatt tgctaactcc ctcacgttac ctggccagct gtattcgcat aaagcatcca
     3301 tagcatcggg cattaatcga acggtaggtc tgccctctcc ttcaattcga gaaaccagct
     3361 cattaaaaag cagtgataaa tcactacttc tattacgtag tggaggcatt tcaatgggaa
     3421 atacattcaa tctgtaaaac aaatcttctc taaatttccc ttcttgaata gcaatttcta
     3481 aatttctatg tgtagcagca atgatacgaa cattgacttc gatgctttta tttgaaccta
     3541 ctctttcaaa acaacgttct tgtaaaactc ttaataactt aacctgcata gctaatggca
     3601 tatctcctat ttcatccagg aatagagttc ctccattggc caactcaaaa cgtccttgcc
     3661 ttgatgtaat cgctcctgta aatgcacctt tttcatgacc aaataattca ctctccaata
     3721 attcccccgg gattgctcca caattgatag ggacaaatgg tttatttgct cgggatgaaa
     3781 gagaatgtat gttgcgagca acgacttcct taccagtacc tgactcaccc aaaatcaaca
     3841 cactggcctc agtatcagct acttgctcaa ttaatttgcg tacttgacga ataccttcac
     3901 tattcccaac caagcttcta aataaaggag tcttatgggt acctgcagtg ataaatttaa
     3961 ctgcttcttt agctatttga cacctatgga gcgatttaag catttttgca tagctgaatg
     4021 gaaatgataa acaatcaacg atatttcgat ccgcgcattc agatgtagaa agcacagcat
     4081 caataaccaa tattggagtt tttggaaact ttttaactaa tgtatccaat tcatccatca
     4141 ttttttcaaa agaagtacac gctccaagaa ctatcacact tggattagga ttaacactca
     4201 attgccaatt gccatattga gtgacctcag agaattgtcc tataaaatca aaaatagtcc
     4261 gaagcttgtc acaacgttcc ttatcatcat cgataatata aatcctgtca ttgctcctca
     4321 taaacctatc cttaagtttg agtcatttaa tttaaagtat cggacacata atgaataact
     4381 gtcaaataat tgtcacaatt tattttaacc gtcataaaat ctatcaaatt aggccgtatc
     4441 gctataatca aaaaaatact aacctatcca gatgctaatt aaaaaatcat agtcatcacc
     4501 agactaagta tttgatttat ctgtaacaaa taatcatctt aaacgcatta cactgattac
     4561 caaaacataa ttcctgactt cctatggtct tcgtagcact cacttgagcc tgcttcacga
     4621 aatccttggc ttgcatatat cccatacgat tacagaacga attggcagct tgtttaccac
     4681 agcctttatt gcgctccata caccaatcca ctcggtaatc attataacgg ggtacggcaa
     4741 attttttctc tcgataatgg taaggtttgg gaggagtatg cgataaacca atagcacaag
     4801 ctattgtcat aaaaccatta caacgccatc ctttgcattg agccttgcta cccaagtaat
     4861 tagttaaacc gacgttatag gcaatgacat tttgagtgga gtaatcataa cccagcattt
     4921 ggcaataacg attcgctacc tcctttccgc actctttccc atctaaagta cagtaatcaa
     4981 gtcgttgccc taaataagta ggatgccaaa aatttctaaa tgccaaattc tgcttacttg
     5041 aagctgttaa ctgaccgcaa aaagaaaaaa ataaaagtat aaaaaccagg aaatttctaa
     5101 aattgctcat tataacaatc cattaccaag tattagcaca tgttagctta ggatgactgg
     5161 gaggcacaag agagatttat tcttctttta ctcgtactgt aatgaacttt tgtcttattg
     5221 cattttctac atacgactat gatttaaaag ctgaccaaag acaaaacaac tatatcaaga
     5281 taatttttca aagactataa acaatgttat aaatagcata tgaacagaac ttatccctgg
     5341 aatcaaggaa acaaatgttt ttaataagaa agtttggcca aattgaatga cagaattaaa
     5401 aacactttgt aacaaagagc ataggggctt aagcaaattc taatgaaaca aacttttcaa
     5461 tcatcacttt aaaagcccat atcaaaggct agtcgtttat gaaaaaatat ttaactttta
     5521 tagtgtcagg cttagcttta attgcaataa tatggttcat tcgccactta tggataattg
     5581 ggactgtcaa ggttgtgcat cctgtttatg gaacagcagt tcaagctgtc tatgccacag
     5641 gaacagtcga accaaccgtc atgatgccaa ttgcctcaca aaatacagcc cgccttgtca
     5701 agttgaatgc agatgagggt tcggaagtag taaagggaca gatattagcc agcctggaag
     5761 atgaacacct gcaacataac ttaaaagaac tgaaagagca tgaaaaatta gcaaaaataa
     5821 gatacgagcg gaatgctcaa ttagttaaaa aaggagtcat ctccaaagat gagtatgatc
     5881 gcaccttgtc cgaatggcaa gcagccagtg ccgcagtacg cagaatagaa gctcaaatca
     5941 gttatttaca actgatagcc cctgcagatg gccgtatcat taaacgtgac ggtgaagttg
     6001 gccaactaat cccggcaaat caacctgtat tttggctttc ttgctgtgct cccctaagag
     6061 tttccgctga agtcgatgaa gaagatatcg tattggtcag agttgggcaa aaagtattga
     6121 tcagatcgga tgcttttcca aatcaaatct tttatggccg tgtcaaaagc atcaccccta
     6181 aaggcgatcc aatagccaga agttacagag ttcgcattag tttttcagaa aataatcctt
     6241 tattgattgg tatgacagct gaaaccaata ttattgtcca tgaaaagaaa aatgccttat
     6301 tgcttcctgt aagtagtatt tctgaaaaca aagtttggat tgtacaggat gatgggaagc
     6361 tctctcaaaa tacagtgact attggcgcaa gaggattaaa acaagccgag attttaaaag
     6421 gcgtcaatat taacgacctg gttgtcttac aacctgataa taagctaaaa aatgggcaaa
     6481 aagtgcgttt cattcttgta aaacaggaag aataatgaag ctatttttaa agatcgcttt
     6541 aaaacatttg cttgcacgca aacgacaaag cctggtttct ttgatgggaa ttgtattggg
     6601 ggtagccttc tttttaacaa tttcgtcact gatgcagggg tctgaaaaag atttcatcaa
     6661 acgcttggtg gataattcac cgcatatcac gatcatagat gaatatcgaa accctcgtat
     6721 acaacccatc caacagctgc acacacatgg cgctattgaa attcgcagtg ttaaaccatt
     6781 aacagaacct cgagggatta gagggtatga acaaacaata aataatttat cgcttaccat
     6841 cccgggaatt gttatttctc ctgttctaat tggacaagcc ttaattagtt atgcagggaa
     6901 agattattcg gtcactctga atggtatgat ccctgaagag ataaaaaaag tttctactat
     6961 cgaaaattat atgacttcag gttcgattga tgatctcata gtcaatcccg atggtattgt
     7021 cttgggaagc gagctcttgc gtaaattatc cctgacaaaa gatgataaca ttactgtgac
     7081 agcgcccaat ggtcaaatac gcactttcaa aattcttggt gtatttcgca ctggccgttc
     7141 agattttgat accaatcaag cgtttattag tattaaacgc gctcaagcat tattaaaccg
     7201 acctaaccgc tccaataaca tcattataaa actgcctaac ccctatcagg cctatgacat
     7261 tgccgctcaa attgaaaaac agataggcta taaaactatt tcctggcagg aaaaatcgga
     7321 agatttgctc aatacactag tcattcgaaa tatcattatg tatagcgtgg ttagcgctgt
     7381 tttaattgtc gctgcatttg gaatttataa tgttatttcg actgtagtaa tggaaaaaca
     7441 cagagacatt gccatactta aatcaatcgg tttccaaaaa catgatattc aatttatttt
     7501 tatcatagaa ggttttcttc taggactggt tggatgtttg ttaggactgc ctttgggaag
     7561 tggactgatg tacagtctca tgcaagtcca attcaagccg ccaggaagct ctgaattgat
     7621 taatatgcca ctcgattgga gttatttaca atttgtcatc gccactgctt ttgcgatgtc
     7681 cgcctcaatc atagccgccg ttcttcctgc tcataaggca gcgcttgttc atcctgttga
     7741 tatcctgcga ggagggggaa tgtgaacaag attgtcataa aaacaaaaga gctttcacgt
     7801 caactgtcag gtgaagttcc cgtcacttta gtcaaagata taaatctcga agtcagagaa
     7861 ggtgaattct tagtaatttc tggcccctca ggttctggca aatcgtcatt gctttatttg
     7921 ctaggcttgc ttgatagacc ctctcaagga acaatttggc tgaataatga agatgtttca
     7981 acttatgagg aagaaaaaat ggcctggttg cgcttgactc aaattggtta tgtctttcaa
     8041 ttccattttc tattacctga gtttacagcc ctggagaatg tgatgttgcc tatgcaacgt
     8101 ctaggcaaac taagtctggg ggaaatgaaa aaccgagcta ccaagctttt aaccagccta
     8161 gggttagagg agcaattatc aaaactgcct aaacagctgt caggagggca aagccagcgc
     8221 gtcgctattg cacgagctct ggccaatgat cccatattgc tcttggcaga cgagccaact
     8281 ggaaatcttg ataccgtttc aagtacgaat gtgcaaaata tactaaaaga acttgctcat
     8341 gaatacagaa gatcggttat catcgtaaca cacgatatga attttgctgc tacagcggac
     8401 agagtgattc acattgtcga tggcaaaatg caagaataac ctcacttctt gctagtccat
     8461 gcttacttca tcagtttcat cctgatctat tcttcaggac taacgaaaaa ccccaaggcc
     8521 aaggcaaaaa atgtttttaa tgagggagtt tagataaact aaatgaccga attaaaaact
     8581 ttttttaaca tagagattgg gggtttgcgt agctcctgtt ctttttaaat cggattagga
     8641 ctatctataa accaatgttc caagtcaaaa tagtttgcaa gataaacacc gagggattga
     8701 atgccgaaac gttccgtcgc atgatgacca caagaaaaat attgtatacc caattccata
     8761 gcctgataga acgttcgttc agaaacttca ccgcttaaat aagcatcaac gccaagttgg
     8821 taagcttctt ctataaagtc ctgtgctgct ccagtgcacc atgctatgga gtgaatcatt
     8881 ttttcatttc ccgcaatatg tacaggatac cggccaagtt tgtgctctaa aaaactggaa
     8941 aactgttcgc tgttcattgc ctttgataat tttccagacc aaaggagatt gggagtattg
     9001 ttaactttat gcatctcatg ggactctatt aacagcaaat tcgccaggct ggcattattc
     9061 cctagctcgg gatgacaatc caaaggcaaa tgataggcaa ataaattcat gttatggcca
     9121 agtagcttag caatccttcg ccttttcatt ccggaaataa caggattttc acctttccag
     9181 aaataaccat ggtgaactaa cagtgcatca gcttgctgtt caatagcttg ggaaatcacg
     9241 tcctctgatg cactgactgc cgtacatata cgcttaatct tgtctttgcc ttcaacttga
     9301 attccattag gtgcataatc ctggtattga gagcaattca agaaatccaa taaaaaaagg
     9361 cttaattcat ctcttgtaat cacctgtcag aaactctctt aatgtccgct cccaatagag
     9421 ataacttttc ttctattcgc tcatagcctc tatcaacatg ataaatccgc tcgactactg
     9481 tttccccttc agcaaccagg cccgcgagaa ttaaactggc tgatgccctt aaatcagtag
     9541 ccattaccgg tgcgccagtt aatttttcaa caccatgaac gatggctgta tttccattaa
     9601 gttggatatt agcccccatt ctctgtaact cttgaacatg cataaatcgg ttttcaaaaa
     9661 tagtttctat aatagttgaa gaaccttctg ctacggaatt cattgccata aattgagctt
     9721 gcatatccgt tgcaaatgca ggataagggg cggttgaaat attaactgcc tgaggccgct
     9781 tgttatgcat atttaaactg acccaatcct caccaatggt taattccgcg cctgcctctt
     9841 cgaatttaca taattgagat aataaagtat caggacgtac tttcttaaca gtcacttgac
     9901 cacgagtaag cgctccagct gccaaataag tgcccgcttc tattctatcg gacatgaccg
     9961 aataagtccc accgtttagt gattccacac cttctacttc aatagtagaa gtacccgcgc
    10021 ctcgaatttt cgctcccatt tgaatcagaa agttagccaa gtctactact tctggttcgc
    10081 gcgcagcatt tttaatcgta gtaatgcctt ctgccaacac agcagccatt aacacatttt
    10141 cggttcctgt gacagtaact gtgtcaaaca tcaagcgttt accttgcaga cgtccctttt
    10201 tgcaacgagc attgatgtag ccatttttaa ccgtaatgtc tgcccccatg gctcttaatg
    10261 cttttaaatg caggtcaact ggtcttgttc caatggcgca tccaccgggc aaagaaacat
    10321 ctgccttacc gaagcgcgcc agcattgggc ctaataccaa aatggaggca cgcattgttt
    10381 tgactagatc gtaaggagca acaaactcat tgacttgact ggagtcaact tgaacattca
    10441 tcttttcatc aacaattaaa tgagctccca attgtcctaa cagttccatc atggtagtga
    10501 tgtctttaag atgaggtaca ttagatattg tgacgtgatc actggctaat agactggcag
    10561 ccatgattgg aagagcagcg ttttttgctc ccgaaatgac cacttcacca tgtaatgctt
    10621 taccaccatt aatcaataat ttatccattt tgcttccccc attcttcttt tgtccatgtc
    10681 ttcatactga tagcatgaag ccttccggtg gtaataaagt ctttaagctg ggcataaacc
    10741 cattgctgtc ttgctacttt agacttctta agaaatatat cggtcactac agttacctga
    10801 tattgataac catcgccttc taccttgaca taaaatacat cgtcaatttc attaagtctt
    10861 tgttcaattt cttcattact taacataaac ttagctcact aattaaaata aatacaaatt
    10921 ttttatactt ttattgataa aaaataatgg acatgactga atcattatca aaaaaatccg
    10981 tgtttacacc atcattctct aaagatttag actgtttcac caaccacaac attacctcaa
    11041 aattcatggg aatttatcaa ttcaggagga caactagagt ctgttaattt ttctctggct
    11101 tgaaacccaa aactttgatt tttaagcacc gtagcagaaa tataaaacca ttattaagca
    11161 acggaacaca gaaaaccaac gctttaaagc caagacagta gaaatgtcga tagattctag
    11221 cgctctaaag ggataagctc tatggaataa taaatatttc tgactcagaa ttgtttcaag
    11281 atctttgtaa aatagtcttc actccacaga attcagccag agattgtgtt tcaggagata
    11341 ttcctattac atcaaaaacc ttattatttt gtttacataa ttttctagct tcaataatta
    11401 aagcaagtcc agcactatcg cagtgagtca cctcgcttaa atcaaggcaa aatctctctc
    11461 tggcatcttc cattaaagcc ttatacagct tgtcacggac cgataccact gatttgaatg
    11521 taagctcagc gccaggttta aagttattaa tttccacgat caggaagcct tcttaatctg
    11581 tttttgttgc atttccttaa tcacttgact aatgctggaa tgttgcagtg cctgtgcaaa
    11641 ttgcgatcta aaactttgca acaaactaac accttcaacg cttatatcat aaattttcca
    11701 tttcccattc ttggacacaa gactataact taatggtata ttttgccctg cagaacgcac
    11761 aatcacgcta tttacacgaa taaaccgact atttaaagag ccgcgtaaag gtaaaaactg
    11821 cacagtttca tcggaatatt gggccaatgg gctagaatat gtacgaatca ccaagcgagt
    11881 aaatgctttt gagaattgaa ccctttccgc ttcagtagct ttactccagg cttgtcttcc
    11941 caagactgaa cgagacatgc cagcaacgtc aacgttgggt aaaagattct cctcgactgc
    12001 tttgtaaata atctcaggat tactttttaa acgcgcttta ttttctttga gaatagaaat
    12061 aatttgatta gcagcacgtt ctaacatagg gataggagag gtctgagcaa ccattgcttg
    12121 agacaacgtc atcaacacaa ctaataaaat tgttttaaaa actctcatgg ttcacctatt
    12181 tttttatatt aaataataat tggcctataa gattttcaag gataattgct tcctgagtct
    12241 tcgcaatggt atccccatca cgtaaatagg gatgatctgt gtcaccgtcc tcgtcaaaac
    12301 cgggaacgat actaatataa ttcgaaccaa gcaagccttc ggtcaaaatt cgtgcagaag
    12361 catcctcata gggaatcttt ttatcactat tcaaagtcat agtcactctt gcattgagct
    12421 cacctggctg caactcaatt cgactgacct caccaatacg aacacctgca acggtcactg
    12481 gagctcgaac ttttaaacca ccgatatcag taaaatctgc agtgacacga taagttttat
    12541 cagacataaa atcagtaatg ttactcactt tcatcgccat tactaataat gctagcagcc
    12601 caagcaacat aaatattcct acactaatgt ctacataacg ttgctttttc atttaccaat
    12661 ctccaatcat cattgcagtc aacaagaaat caagacctaa tacagcaagc gatgagtata
    12721 ctacagtttt agttgtagca tgactgatgc cttcagctgt tggcacacac tcaaaacctt
    12781 ggtatacaga aatccaagta accacaaatg cgaaaaccaa gcttttaata atcccgctta
    12841 agatatctat acgaaaattt acagcggctt gcatattaga ccaaaaactc cctgcatcca
    12901 cgcctagcca gtaaacacca ataaaataac caccatatat agctactgca gaaaaaatca
    12961 aagcaagtat cggtaacgct ataattcctg ccacaaaacg cggataaacc actcttccta
    13021 atggatccac tcccatcata tccatactgg ataactgctc tgttgccttc attaaaccaa
    13081 tttcagctgt aagggccgac ccggcacgcc ctgcaaacaa cagagcgcta atcacaggtc
    13141 ctaattctct ggcaacgctt aaagccaata attgacctaa ttgacttgaa gcaccaaatt
    13201 tttgtaacgt attatacccc tgaagtccta caaccatacc aataaacaag gctgaaacca
    13261 caataattaa acaagacaat acaccgataa aataaagctg ataacgtaat aatggccata
    13321 accttaaaac attaggcttt ctgaacaaca tggaaaataa aaaaagccct gaactaccaa
    13381 taccttgtaa ggcttgtaca cctcgattac ccagacgcgc gattgtatca agcatttaat
    13441 aactcctctg tataaggttt tgccggataa tggaaaggca ctaccccatc cgcttctcca
    13501 tgcataaact gtcttatctg aggctcaata gatcgcttta attcatccgg agtaccttga
    13561 cctataattt taccccctga tattaagtag atataatctg caattgaaca agtttcttca
    13621 acatcatgag atacgataat agtggtggta tgcaataatt gattcaatcg ttgtattaat
    13681 ctgaccaata cccccatgga aattggatct tgaccggtaa aaggctcatc atacatcatt
    13741 aatacaggat caagagcaat ggttctggct aatgcaactc tcctgcacat tccccctgat
    13801 aattgagcgg gcattagatg ggcggctcca cgcaaaccaa ccgcttcgag cttcattaat
    13861 acaatgtttc ttatcatgga cgcgtttaat tcagtatgtt ctcttaatgg aaaggcaaca
    13921 ttttcaaata ctgaaaggtg tgtaaataag gcactacttt gaaaaagcaa tcccatgttt
    13981 cgcctggttt cataaagtga tttgcgggat aattgatgaa tattctgacc attaacataa
    14041 atttggccac tatcaggcct caattgcgca ccaattaacc gcagtaaagt agtctttcca
    14101 gaaccactgg gtcccataat agcggtaatc ttgccttgtt taactaccat atcaatgcca
    14161 tcaaaaatgc agcgatcatc aaggctaaag aatagatttt ttatttcaac ccaattatca
    14221 ggcatgcttt attatccact tattatttta acgggtaatg atgtcacttt gatatctatc
    14281 aaagacaggt aatgctttat tatatcttta attaagaagt atatactgga taaataaaaa
    14341 tgtgtttaat aattgcgttt ccattaaaaa aaaacttttt aaaagttttt ttacaatacc
    14401 aatttttcaa aaattaatca gaaaatttaa ataaaaggta ttttttat
//
LOCUS       DASLOE010000069        14314 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.069, whole genome shotgun sequence.
ACCESSION   DASLOE010000069 DASLOE010000000
VERSION     DASLOE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14314
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.069"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            30..1298
                     /gene="icd"
                     /locus_tag="U6309_001626"
     CDS             30..1298
                     /gene="icd"
                     /locus_tag="U6309_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEN5505067.1"
                     /translation="MESSMTYDKIKVPAQGEAITVAADHSLHVPDNPIIPFIEGDGIG
                     VDVTPPMIRVVDAAVQKAYGNKRKISWMEVYAGEKATKVYGSDQWLPKETLDAMKKYV
                     VSIKGPLTTPVGGGIRSLNVAIRQDMDLYVCLRPIRYFNGVPSPVREPWKTDMVIFRE
                     NSEDIYAGIEWQADTPEAKKVIQFLTKEMGVKKIRFPEHCGIGVKPVSREGTTRLVKA
                     AIQYAIDNDRSTVTLVHKGNIMKFTEGAFKDWGYQVARDSFGAKEYQGGPWMEFKNPK
                     TGKQIIINDVIADAFLQQILLRPEDYSVIATLNLNGDYISDALAAQVGGIGIAPGANI
                     SDQMAVFEATHGTAPKYAGQNKVNPGSIILSAEMMLRHMGWYEAADLIIRGMEGAIEA
                     KTVTYDFERGMQGATLVSSSGFADAMIKHM"
     gene            1700..2035
                     /gene="clpS"
                     /locus_tag="U6309_001627"
     CDS             1700..2035
                     /gene="clpS"
                     /locus_tag="U6309_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEN5505068.1"
                     /translation="MSRQNLEEIIQTGIADTELSTEISTAIKRPRKYKVLLLNDDYTP
                     MDFVVEVLKHFFHLNEEVAIQVMLQVHFQGKGVCGVFTRDIAETKVALVNEYARMNQH
                     PLLSSMEPE"
     gene            2067..4334
                     /gene="clpA"
                     /locus_tag="U6309_001628"
     CDS             2067..4334
                     /gene="clpA"
                     /locus_tag="U6309_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEN5505069.1"
                     /translation="MLNKELEFTLNLAFKEAKEKRHEFMTVEHLLLSLLDNPAAGNVL
                     QACDANIEALRRDLIEFIDETTPRIPEDELDRETQPTLGFQRVLQRAVFHVQSAGKTE
                     VTGANVLAAIFSEQESQAVYFLRRENITRLDVINYISHGVSKYQNNDMHENMNSSMEE
                     DMMTPEGNESPLDSYCTNLNRRAKQGKIDPLIGRHEEIQRTIQVLCRRRKNNPLLVGE
                     AGVGKTAIAEGLARRIVDGEIPDSIRGCVVYSLDLGALLAGTKYRGDFEKRLKAVLKQ
                     LGQQEGAILFIDEIHTIIGAGAASGGVMDASNLVKPLLANGELKCIGSTTYQEYRGIF
                     EKDRALARRFQKIDISEPTIDETFEILKGLKGKLEAHHGVKFSIASLKAAAELSAKYI
                     NDRFLPDKAIDVVDEAGAYQNLLTANKRKKIISVTEIESVVAKIARIPIKKVSARDKD
                     TLRNLERDLKLLVYGQDQAITALASAIKLGRSGLREPQKPVGCFLFAGPTGVGKTEVT
                     RQLANVLGIELLRFDMSEYMEKHTVSRLIGAPPGYVGYDQGGLLTEAVTKNPHAVLLL
                     DEIEKAHPDVFNLLLQIMDHGTLTDTNGRQADFRHIILVMTSNAGASEIVRNSIGFST
                     QDNTNDGLEVIKKQFSPEFRNRLDAIINFAPLDTQTIGLVVDKFIMELDEQLSHKGVT
                     FTVDKSAREWLMEHGYDKAMGARPMARLIQENIKKPLADELLFGKLMHGGHVTLRVKD
                     GKLHFDSHDHREGVC"
     gene            4558..5337
                     /locus_tag="U6309_001629"
     CDS             4558..5337
                     /locus_tag="U6309_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN5505070.1"
                     /translation="MLSVIIITKNEEANIRRCLESVHFADEIIVLDSGSTDNTLAIAR
                     EYTDKVFSTDWQGYGIQKDRALQKAQGDWVLNLDADESVSSELRQEMLQAISSDTADA
                     FRIPIQMIFYNQVLKYSGSPKRHIRLFKRENACYSKDIVHEKVLIPVNARVGKLKKPI
                     WHHSFSDVHHVLYKLNKYSSYSAKIRIESNEKVGLVKTFFSALWMFVRCLFLQKGFLD
                     GRAGFLFAVFGAQGAFYRGVKQIYKDRDINKLPSVNHIKEE"
     gene            5340..6590
                     /locus_tag="U6309_001630"
     CDS             5340..6590
                     /locus_tag="U6309_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEN5505071.1"
                     /translation="MRLTIMDTKGILLAPFFFISFMFFIPISPTIKSICFICSLIAVL
                     ITPQYRKHIVYAYNTLWGRAAFCLFLFVVIASLWSPAPYSMQWMVIGKYCKLIYLPIL
                     ATGFINPKVRNWSINSYLAAIFITCVISILKAKNIIASEDPGEVFYNHIITGFMVALA
                     AYLTGLLAFQNSGWLRLIYIFMMFLTSYQVLFINTGRTGYLVYFILMLLLLIQKLNFK
                     QALAGIVLFSGIIALAYHHSPVMQTRVYDLIQDIKLLKQHNKNTSIGYRMQFHHYAKQ
                     LMLKHPFIGIGTGGFKYSYSQDVPVPSWGKELTDPHSQYWMTLAEQGLIGLVLLVIFL
                     ASLFITSFQLTSSRPILLGVLFSFCIGSISDTILCYSTAGYLLVVMSALCFGELIEKR
                     RVKGVEEDHLVPLSAHALAAISQT"
     gene            complement(6591..6965)
                     /locus_tag="U6309_001631"
     CDS             complement(6591..6965)
                     /locus_tag="U6309_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurogenic locus notch"
                     /protein_id="HEN5505072.1"
                     /translation="MLFLKIKTNQRTTMNILKPKAFLLASVFALSASPAFAADGCCSK
                     MGGINYCDSSAGRLVCNNGFYSTCYCTRHAVMDLQFLMGCCLWHGGVYPQLNSSGLVV
                     CNDGYVSEECSLQKPVEQISVY"
     gene            complement(7096..7692)
                     /locus_tag="U6309_001632"
     CDS             complement(7096..7692)
                     /locus_tag="U6309_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN5505073.1"
                     /translation="MINELTASLNMIVTQTQNNLKILGVILLIPWSIFFISYFISNKI
                     LLLGIIPRHILGLPGILLAPLLHANFNHLFFNSIPLLVLSNFILINGVNYYLIVTVMI
                     TLFSGTLIWCFAKRGIHIGASGLITGYWGFLVYNIYQTGTLTTIILGLLSLYYFAGIF
                     LGIFPGEKGVSWQGHLFGLLAGFLTSYLLEVYPQILIY"
     gene            complement(7689..8591)
                     /locus_tag="U6309_001633"
     CDS             complement(7689..8591)
                     /locus_tag="U6309_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN5505074.1"
                     /translation="MKKLLHSLACLFILVNSAKLSYALALPENHSVNGGLTIIPIDIN
                     QKPEAYFEGKRIPVLPSPQPRQWLLIVAIPLKNTNSVQYINVTKPIKAAIPFHVSEKF
                     YSTQFLNIKDISKVDPQPEDLIRIEKETKKLTQIFANYSNANPFEKQFTAPLRGPITS
                     LFGLKRVYNKQPRDPHSGLDIAADEGKPVYAVNQGVVADTGDYFFTGNTVILDHGMGV
                     FSVYAHLSKILVKTGETVKQGQELGLVGMTGRVTGPHLHWTMVVNQTLVEPLLFVPFR
                     KITVTPVIPDKKSNKSTQDATVKS"
     gene            complement(8600..9931)
                     /locus_tag="U6309_001634"
     CDS             complement(8600..9931)
                     /locus_tag="U6309_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEN5505075.1"
                     /translation="MSGQLPIMTVSQLNRQVKGFLENEIGLVHVEGEISNLSKPSSGH
                     YYFTLKDSTAQIRCAFFKNRHSSSLLRNFNDGQQIVASGKLSLYEARGEYQLIVEEIV
                     EAGMGILYQRFEELKIKLASEGLFNPERKKTLPRIPETIGIITSPTGAAIQDILSTLA
                     RRFPIARIIIYPSEVQGQTAPQQLVNALKLANTHKRCQVLILARGGGSIEDLWAFNDE
                     YLARQIAISEIPVVSGIGHETDFTIADFVADYRAETPTAAATAVTPNCIELFNILDTA
                     IYRLHDAIIRLVKGLQLKLNHLIDKIASPKQTISTYWQTLDYLERQLISAMTQFINLK
                     INKVNLFSTQLQANNPKTQIERTKIQLRQLIMQLTQEIQIKVNQLKNQLSTNLSTLHA
                     VSPLATLDRGYAIVSKNQRILFAAQHVQIGDTIDVRLAKGSLACEVTQIKD"
     gene            10088..11812
                     /locus_tag="U6309_001635"
     CDS             10088..11812
                     /locus_tag="U6309_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEN5505076.1"
                     /translation="MKKALLVAALLTSSYATADTLYEAVQYGMIANPDILLNTAKGLS
                     AKQAIDKAKGGLYPSIDVTGGFGRQRSINPTSAAIDDTPSTTLNIVESAVELRQRLFA
                     GGGIINEVKRNQYLTEAQKWKTQGIAEDLALEITKNYFAVLLHERLYAYSIENLKAHK
                     AVFKMIKERATAGITRAAEVDQANARLALAEANKISALADLQEVKINYAKTVGKWPEN
                     LQMPRVPARNSLPNNLARIIEKGLDNHPTVKSSYADIKEAKAQYDVARAAYYPEVNLV
                     LNSSKNKNLGGLAGPNDSDTVALRMNYNAFRGGADAAHIRETAYQVQEAYETKNRTLL
                     ELKETIRLAWNAYVASALRIQPLKQHVTASRKTRTAYQDEFKVGKRTLLDLLDSQNEY
                     YESQIELARAENDEILSRYRILNGMGCLLKYLNLRLPVNVVNNDVFSSAQTNILLNRK
                     MDEIPYPNNTDNPMVLTHPVKNMEKTRLTKSMVDKNTTYPQQITPKLWYISAGSFRDK
                     NNAVALVKRLNGLGFTAFMSPYKDLLSVFVGPYEYRGHAGNGMERLKELAHVEGVLVT
                     FKEPPKKA"
     gene            11763..12497
                     /locus_tag="U6309_001636"
     CDS             11763..12497
                     /locus_tag="U6309_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEN5505077.1"
                     /translation="MLKESWLLSKSRPRRLSVIAKRFQNVLLESLRYRLVLSIWTGLI
                     LCYITSFSTQAAPLISVEKIQKLAQSYQGDTRKRFTAWANLIDSLKKKPVKIQLEKVN
                     SFFNQFNYETDPITGASDDYWKSPVEFIVDGGGDCEDFAIIKYFTLVAVGVPSDQLRI
                     TYAASLTLNQAHMVLSFYPTPESEPLILDSLESKILKASARPDLKPVYSFNAEGLWLA
                     KTRGESSLMGDSKSLGKWDALMKRME"
     gene            12513..>14314
                     /locus_tag="U6309_001637"
     CDS             12513..>14314
                     /locus_tag="U6309_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEN5505078.1"
                     /translation="MTLTKKMAVGVLIMLLFVFIGTYFITMNNARNFFIQQLESNAQD
                     TATSLGLSLSQSLINHDVPTMDSMVKAVFDRGYFSSIKVKDIKGKVIILKKQLPQESD
                     IPQWFVNLIKWPSTEKSSLIMDGWMQAGVVLVASDPSYVYASLWRNAVEMVNAYLIFA
                     LVALVVSYCFIKYLLQPLKRVTAQALAISEHEFPVETKIPKTPELRQVTLAMNQMVTK
                     VKSLFQDQLKQTESLRTQVYQDSLTGMSNRRYFLQHLALILDNEDEFIPGYVMMLVID
                     GLDELNQKQGYQQGDQLVLTVAKICKSYWKQSSVSTLARINGTTFALISHERDPLVFE
                     KECKEFEQILSQGITDIKICKTYLGAANYFLHQPVSNLLSMVDQAVKKARETGVFYCQ
                     KEHDTYKYPQLISGDEIRNSLEQKKISLYAQAVTDGKNCFHKEVFVRIRNQEGEELGA
                     GYFIPVAEKLGLAYLIDQYVLNELTVMDIATHTHFALNISEDTLANKVNSTGYLRQLE
                     DTPAAVLRNLSLEINEAHVLSHFSNSKFFIKQAKKLGVTVGIDRVGIKFSPLHYLSDL
                     NIDYLKLHGSLVTDIDENESKQFFIHYFNEMAKT"
BASE COUNT         4476 a         2423 c         3040 g         4375 t
ORIGIN      
        1 cacatgctat cattaatttc tctaaattta tggagtcgtc aatgacatac gataaaatca
       61 aagtgcctgc ccaaggcgaa gctattactg ttgctgctga ccattcactt catgttcctg
      121 ataatccaat tattcctttt attgagggtg atggtatcgg tgttgatgta acacctccga
      181 tgatccgtgt tgttgatgct gcagttcaaa aagcatatgg caacaagaga aaaatttcct
      241 ggatggaggt ttatgccggg gaaaaggcta ctaaggttta tggtagcgat caatggcttc
      301 ccaaagagac tctggatgcc atgaaaaaat atgtggtttc aattaaaggt cctttaacta
      361 ctcctgttgg tggcggtata cgttcattga atgtcgctat tcgccaagac atggatctct
      421 atgtttgttt acgtccaatt cgctatttta atggtgttcc aagcccagtg cgggagccat
      481 ggaaaaccga tatggtcatt ttcagggaaa attccgaaga tatttatgcc ggtattgaat
      541 ggcaggcaga taccccggaa gctaaaaaag tgattcaatt tttaactaag gaaatgggtg
      601 ttaagaagat tcgttttcct gagcattgtg gtatcggtgt aaagcctgtt tccagagaag
      661 gtactactcg acttgtcaaa gcggccattc agtatgcgat agataatgac cgcagtacag
      721 tgactctggt acataaaggc aatatcatga agtttactga aggggctttt aaagattggg
      781 gatatcaggt tgctcgcgat tcttttggag caaaagaata tcaaggtggg ccatggatgg
      841 aatttaaaaa tcctaaaacc ggtaagcaga ttatcattaa tgatgtgatc gctgatgcct
      901 ttttgcaaca aatcctgtta agacctgaag actatagtgt cattgctacg ttaaatttga
      961 atggtgatta tatttctgat gctttggctg ctcaggttgg gggaatcggt atagctcccg
     1021 gagcgaacat tagtgatcaa atggctgtgt ttgaagcaac tcatggaaca gcccctaaat
     1081 acgctgggca gaataaagtg aaccccggtt ctattattct ttctgccgaa atgatgttac
     1141 gtcatatggg gtggtatgaa gcggctgatt tgattatcag aggtatggaa ggcgcgatag
     1201 aagccaaaac cgtaacttat gattttgaac gtggtatgca aggagcaacg ttagtcagca
     1261 gctctggttt tgcagatgct atgattaagc atatgtaaac cggggagtta tcacatggtc
     1321 tttctgaaca acgcgtattg acaatatagt gcttacagcg ttactcagtg ccaatgcgtt
     1381 gttcatgaaa gactgggatt atcctttcaa gattattggt agtatctttg actatcaggt
     1441 ttatacatct tacgagttca tgaaaattga gcaggaactt tatccatgct aattatgatg
     1501 ctactctact gattagagac ggctcaatct cttgagtaaa acctggtggt caataacaaa
     1561 tagtacaaca tttgagtgaa tcatatctaa tcactatgct attgattatt tcaatgacta
     1621 aatttttcat taaaattagc taaatttact ggccaaatgc gtgtagggag tctataaaaa
     1681 tagtaagagg ttgttagtta tgagcaggca aaatttagag gaaataatac aaacagggat
     1741 agctgataca gagcttagca cagaaattag cacggctatt aaacggccaa gaaagtataa
     1801 ggttttattg ctcaatgatg actatacgcc aatggatttt gtggtagagg tactgaagca
     1861 cttttttcat ttaaatgagg aggttgcgat tcaggtgatg ttacaggttc attttcaagg
     1921 gaagggggtt tgtggagtgt ttacacgaga catagcagaa acaaaagtcg ccctggtaaa
     1981 tgaatacgcc agaatgaatc aacatccatt attatctagt atggaaccgg agtaaactgg
     2041 tggtgggttg gtaaggagca atgacaatgt taaataaaga actagaattc accttgaatc
     2101 tagctttcaa ggaagcaaaa gaaaagcgtc atgagttcat gactgttgaa catttgctgt
     2161 tgtctttgct tgataatcca gcagcaggaa atgtattaca ggcttgtgat gctaacatcg
     2221 aggcattacg tcgtgattta atcgaattta ttgatgaaac aaccccaaga attcctgagg
     2281 atgaactgga cagggaaact caaccaacat taggttttca aagggtttta cagcgtgcag
     2341 tatttcatgt gcagtcagca ggtaaaacag aagtcactgg agctaatgta ttagccgcaa
     2401 tttttagtga gcaagaaagt caggctgtat attttttgcg acgtgaaaat attactcgcc
     2461 ttgatgtcat taattatatt tctcatggag tgtcaaaata tcaaaacaac gatatgcatg
     2521 aaaacatgaa ttcgtctatg gaagaagaca tgatgacccc tgagggtaat gaatctccac
     2581 ttgatagtta ttgcaccaat ctcaacagac gcgcaaaaca gggcaagata gatccgttga
     2641 tagggcggca tgaagagata caaagaacta tacaagtgct ttgcagacgt cgtaaaaata
     2701 atcctttatt agttggcgag gcaggtgtag gtaaaacggc tatcgcagaa ggtttggcca
     2761 gacgcattgt ggatggtgaa atcccagatt ccataagagg ctgcgtagtc tactctcttg
     2821 atttgggcgc cttattggca ggaacgaaat acagagggga ttttgaaaaa cgattgaaag
     2881 ctgtgctgaa acagttgggg cagcaagaag gagccatttt attcatagat gaaattcaca
     2941 ccattattgg cgcaggcgct gcatctggag gagtcatgga tgcttccaac ctggttaagc
     3001 cattattggc aaatggtgaa ttgaaatgta ttggctcaac cacttatcag gaatatcgtg
     3061 gaatatttga aaaagacagg gccttggcaa gacgattcca aaaaattgat atttcagaac
     3121 caacaatcga tgaaactttt gaaatattaa aaggattaaa aggcaaattg gaagctcacc
     3181 atggagtaaa attttcaatt gcttcattaa aagcagcggc agaattatct gcaaaataca
     3241 tcaatgatcg gtttttgcct gacaaagcga ttgatgtggt ggatgaagca ggcgcttatc
     3301 agaatttact gacagcaaat aaacgtaaaa aaattattag cgtgactgaa atagagagtg
     3361 ttgtcgcaaa aatcgctaga atcccaatta aaaaagtatc tgcacgcgat aaagatactc
     3421 tgcgtaacct ggagcgtgat ttaaaattat tagtctatgg gcaagaccag gcaattactg
     3481 ccttggcttc agcaatcaaa ctgggtagat ccggacttag agaacctcag aagccagtag
     3541 gttgtttctt gtttgccgga ccaactggtg taggaaagac agaggtaaca aggcaactgg
     3601 ccaatgtgtt aggcatagag ttgctgcgtt ttgatatgtc agagtacatg gaaaaacaca
     3661 ctgtttcacg tttgattgga gcacctcctg gctatgtagg atacgatcaa ggtggtttat
     3721 tgactgaagc cgtgactaag aatccacatg ctgttctgct acttgatgaa atagaaaaag
     3781 cacatcctga tgtttttaat ttattgttgc agataatgga tcatggcact ttaactgata
     3841 ctaacggtcg tcaagctgat ttcagacata tcatacttgt aatgaccagt aacgcaggag
     3901 ccagtgaaat tgttcgtaac tccataggtt tttcaacgca agacaatacc aatgatgggt
     3961 tggaagtaat taaaaagcaa ttcagtcctg agtttaggaa ccgtttggat gctattatca
     4021 attttgctcc gttagataca cagactatag gactagtcgt tgataaattc attatggagt
     4081 tggatgaaca attaagtcat aaaggcgtta cttttacagt tgataagtca gccagggaat
     4141 ggctgatgga gcatggttat gataaagcca tgggagcaag acctatggcc agattgattc
     4201 aggaaaacat caaaaagcct cttgctgatg aactgttgtt tggtaaatta atgcatggcg
     4261 gtcatgtcac tttgcgagtg aaagacggta aactgcattt tgacagtcat gatcatagag
     4321 aaggtgtttg ttaaacggga accatcttca gttctgtgca tgacaaattt tttgttatgc
     4381 acagacaaaa tcacaataat cctgagatag taattgtcat taacctgtta gttgcataac
     4441 tggtaattgt tatcacgatt tctttttgtg accgctaaac gaatattatt cctttttacc
     4501 ttgttacaat ccggcttaat tatcgatata atatttattt tgaattgata taaagccatg
     4561 ttatccgtaa tcattatcac aaaaaatgaa gaagccaaca taaggcggtg cttggaatcc
     4621 gttcattttg cagatgaaat aattgtattg gactctggca gcacagataa tacattagct
     4681 atagccaggg agtatacgga taaagtattt tcaacagatt ggcaaggcta tggtatccaa
     4741 aaggataggg ccttacagaa ggctcaaggg gattgggttt taaatcttga tgctgatgaa
     4801 tcagtaagct cggaattacg acaggaaatg ctacaggcca tttcatcgga tacagcagat
     4861 gcatttcgta ttcctataca aatgattttt tataatcaag ttttaaaata ttcaggaagt
     4921 cctaaaaggc atatacgatt atttaaaaga gagaatgctt gttatagcaa agatatcgtt
     4981 catgaaaaag tattgattcc tgttaatgcg agagtgggga aactaaaaaa acctatttgg
     5041 catcattctt ttagcgatgt acatcatgtg ctatacaaac ttaacaaata ttcttcttat
     5101 agtgcgaaaa tccgtataga atccaatgaa aaagttggat tagtaaaaac tttttttagt
     5161 gccctatgga tgtttgtcag atgccttttt ttacagaagg gatttttaga cggcagggca
     5221 ggttttttgt ttgcagtatt tggtgcgcaa ggcgcttttt atcgcggggt caaacaaata
     5281 tataaagaca gggatatcaa taaattaccc agtgtaaatc atataaaaga ggaataattg
     5341 tgcgattaac cataatggat accaagggta ttttattagc tccttttttc tttatctcat
     5401 ttatgttttt tattccgatc agtcctacga taaagtctat ttgtttcatt tgcagtttaa
     5461 ttgctgtatt gattactcca caatacagaa aacatattgt ttatgcttat aatactttgt
     5521 ggggacgcgc tgcattttgt ttgtttctct ttgtcgttat tgcaagttta tggagtcccg
     5581 ctccttattc aatgcaatgg atggtcattg gtaaatattg taaattaatt tatcttccca
     5641 ttttagctac tggttttatt aatcccaaag taagaaactg gagtatcaat agctatttgg
     5701 ctgctatttt tattacttgt gttatttcca tcttaaaggc taaaaatatt attgcgtcag
     5761 aagatccggg cgaggttttc tacaatcata ttattactgg ttttatggtg gcattggcag
     5821 cctatttaac aggattgctg gcttttcaaa attcgggctg gttacgactt atttatatct
     5881 tcatgatgtt tctaaccagt taccaagtgc tttttataaa tactggaaga acgggttatt
     5941 tggtctactt tattttgatg ttattgcttt tgatacaaaa attaaacttc aaacaggctt
     6001 tagcaggaat tgttctcttc agtggcatca ttgctttggc atatcatcat agccctgtaa
     6061 tgcaaaccag agtttatgat ttaattcagg atatcaagtt attaaaacaa cataataaaa
     6121 atacatcgat aggctataga atgcagtttc atcactatgc caagcaatta atgttgaagc
     6181 atccttttat tggaataggc acaggtggat ttaaatacag ttattcacaa gacgtacctg
     6241 ttccaagttg gggtaaagag ttgacagatc cacacagtca gtattggatg actttggctg
     6301 agcaaggttt aattggtctg gtacttttgg taatattttt agcgagtttg tttataacat
     6361 catttcaatt gactagctcc agacctattc ttttaggtgt tttattttct ttttgtatag
     6421 gctccatttc cgacacgatt ttatgctatt cgactgcagg ttatttgttg gtagtgatga
     6481 gtgccttatg ttttggtgag ttaattgaga agcgtagagt caagggcgta gaagaagacc
     6541 atcttgttcc tttatcagcc catgccctag cagcaataag tcagacctaa tcaataaaca
     6601 gaaatttgct caacaggttt ttgcaatgaa cattcttccg aaacataccc gtcattgcaa
     6661 accacaagac ccgatgaatt taattgtgga tagaccccgc catgccataa gcaacatccc
     6721 ataagaaatt gtaaatccat tacggcatga cgtgtgcagt agcaagttga atagaaaccg
     6781 ttgttacaaa caagacgacc agcagaagaa tcgcaataat ttattccccc cattttactg
     6841 caacaaccat cagcagcgaa agcaggagat gctgacaaag caaacacact ggctagtaaa
     6901 aatgcttttg gtttcaatat gttcattgtg gttctctgat tggtttttat ttttaagaat
     6961 agcatagagt tagatggatt attagtgaaa tatgatgatt taattgcagt ttaagactga
     7021 atttattatg acaaaaaagt tcaaagtgaa atgataaact ggtttcatgg gccagctatt
     7081 gtgaagagtt aatgattaat aaatgagaat ctgaggatag acttctaata aatagctggt
     7141 gagaaaacct gctaatagcc caaacaagtg gccttgccag gatacgcctt tttcacctgg
     7201 aaaaattccc agaaaaatac cagcaaaata atataggcta agcagaccaa gaataatagt
     7261 ggtcaaggta cctgtttgat aaatgttata caccaggaat ccccagtagc ctgtgattaa
     7321 accactggca ccaatgtgta tacctcgttt agcaaaacac caaatcagtg ttccgctaaa
     7381 aagagttatc atcactgtga caatcaaata atagttaact ccattaatca gaataaaatt
     7441 acttaaaacc agtaaaggga tagagttaaa aaacaggtga ttaaaattag cgtgaagtaa
     7501 aggtgctaat agtatccccg ggaggcccaa aatatgcctt gggataatgc caagaagcaa
     7561 aattttatta cttataaaat aactaataaa aaatattgac cagggaatta gtagtattac
     7621 tcccaaaatt ttcaggttat tttgtgtctg agtgacaatc atattcaaac tggccgttaa
     7681 ttcgttaatc atgattttac cgttgcatct tgtgttgatt tgtttgattt tttatctggt
     7741 ataactggtg taactgtgat tttgcgaaac ggtacaaaaa gtaaaggctc aaccaaagtt
     7801 tggttaacta ccatagtcca gtggagatgg gggcctgtta ctcttcctgt cataccaact
     7861 aatccaagct cttggccttg tttgactgtt tctcctgttt taaccagaat tttgctaaga
     7921 tgagcataaa ccgagaatac tcccatgcca tgatcaagga taaccgtatt gcctgtgaaa
     7981 aaataatcac cggtatctgc tacaactccc tgatttacag catacacagg tttcccctcg
     8041 tcagcggcga tgtccaatcc agaatgcgga tctcttggct gtttattata aactctcttc
     8101 aggccaaata aactggttat aggtcctctc agtggtgctg tgaattgttt ctcaaatgga
     8161 ttggcattgg agtaattagc aaaaatttgg gttaactttt tggtttcttt ttcaatccgg
     8221 attaaatctt cgggttgagg atccacttta ctgatatctt ttatatttaa aaattgggta
     8281 ctgtaaaatt tttcactaac atgaaaggga atagctgctt tgatgggttt tgtcacattg
     8341 atgtattgaa cagagttggt atttttaaga gggatcgcaa cgattaacaa ccattgccgg
     8401 ggttggggac tgggaagaac agggatacgt tttccctcaa agtaagcttc aggcttctga
     8461 tttatatcta taggaataat agtgagcccg ccattgacag aatggttttc tggcagagcc
     8521 agggcatagg atagcttggc tgaattcacc aatataaata gacaggctaa tgaatgtaat
     8581 agtttcttca tgattttaat caatccttaa tttgggtgac ctcacaagca agacttcctt
     8641 ttgctagacg gacatcgata gtatctccaa tttgtacatg ttgggcagca aagagtattc
     8701 tttggttttt actcacaata gcataacctc tatctagtgt agccaagggg cttacagcat
     8761 gcaaagtgga aagattagtg gatagctgat tttttaattg gtttactttt atctggatct
     8821 cttgggttaa ttgcattatt aattgtcgta gttggatttt agttcgttca atttgtgttt
     8881 ttggattgtt agcttgcaat tgtgttgaaa aaagatttac cttgttaatt tttaagttaa
     8941 taaattgagt catagcagat attaactgtc tctccagata atccagggtt tgccaatatg
     9001 ttgagatagt ttgcttgggt gaggcaattt tatctatcaa atgattgagc tttaattgca
     9061 accctttaac cagtctaatg atggcatcat gtaatcgata aatagccgta tcaagaatat
     9121 tgaataattc gatacagttt ggagtaacag ccgtagcggc agcagtaggc gtttctgcac
     9181 gataatctgc tacaaaatca gcaattgtga aatctgtttc atgaccaata ccggaaacaa
     9241 caggaatttc gctgattgca atttgtcgag caaggtattc atcattaaag gcccacaagt
     9301 cttcaatgct gccaccgccg cgcgccagga tgagaacctg gcatcgttta tgggtattag
     9361 ccagttttaa ggcattgact agttgttgag gggcggtttg cccttgaacc tcacttggat
     9421 aaataataat tctggctatt gggaatcgac gggccagagt tgagagaatg tcctggatag
     9481 ctgctccggt gggtgatgta atgatgccaa tcgtttctgg tattctgggc agtgtttttt
     9541 ttctctcagg attgaataag ccttcgctgg ccagtttgat ttttaactct tcaaatcgtt
     9601 gatagagaat ccccattcct gcttcaacta tctcttcaac aataagctga tattctcccc
     9661 gagcttcata cagacttaat ttgccactgg caacaatttg ttgaccatca ttaaaatttc
     9721 taagcagaga actggaatgt cgatttttaa aaaaggcgca acgaatttgc gctgtgctgt
     9781 ctttcagggt gaaatagtaa tgtcctgaag aaggtttgct taagttagag atttcgcctt
     9841 cgacatgcac aaggcctatt tcattctcga gaaaaccttt cacttgtcta ttcagttgac
     9901 tcacagtcat tattggcaat tgaccactca tagtgtaaaa aattccatca aatcaaaggg
     9961 ttattaacat caataactaa ttaaaagaaa taaaattata ttgtagtaaa acattctttc
    10021 aattattgtt atacgttacc atatcaacaa ttaataaaat aacaataata gttaaaaagg
    10081 atggggaatg aaaaaggcgc tgttagtagc tgcattgtta acttcttcct atgctactgc
    10141 cgatactctc tatgaagcag tacaatatgg catgattgct aacccggata tactacttaa
    10201 tactgcaaaa ggtttgtcag cgaaacaggc aattgataaa gcaaagggtg gcttatatcc
    10261 ttctattgat gtaactggcg ggtttggacg acaaaggagt attaacccga catctgcagc
    10321 aatcgatgat actcccagta ctactctcaa tatagtggaa tcggccgtgg agttaaggca
    10381 gcgattattt gctggtggag ggataattaa tgaagtgaag cgaaaccaat accttactga
    10441 ggcgcaaaag tggaaaacac agggtatcgc tgaagatttg gccttggaaa ttaccaaaaa
    10501 ttatttcgct gtattactgc atgaacgttt gtatgcttac tccatcgaaa atcttaaagc
    10561 acataaagcg gtttttaaga tgataaaaga acgggctacc gccgggatta cccgagcggc
    10621 agaagtcgac caggctaatg caagactagc tctcgctgag gctaataaaa taagcgcatt
    10681 ggctgatttg caagaggtga agattaatta tgcaaaaaca gtaggtaaat ggccagagaa
    10741 tcttcaaatg ccccgtgttc cagcaaggaa tagtttacct aacaatttag ccaggattat
    10801 agaaaaagga ctggacaatc atcctacagt gaaatcaagt tatgctgata ttaaggaagc
    10861 aaaagctcag tatgatgttg ccagggcagc ttattatccc gaggttaatc tggttttgaa
    10921 tagctcaaaa aacaagaact taggtggtct ggccggcccc aacgattctg acacggttgc
    10981 tttaagaatg aattacaatg catttcgtgg aggagctgat gctgcgcata taagagaaac
    11041 cgcgtatcaa gtccaggaag cttatgaaac aaaaaatcgc acattgctgg aattaaaaga
    11101 gactatcagg ttagcctgga atgcttatgt tgcctccgcc ttgcgcatcc aacctttgaa
    11161 gcaacacgtt acagcttcga gaaaaacacg tacggcctat caagatgaat ttaaggtagg
    11221 taaaaggaca ctcctcgatt tactcgactc tcaaaatgaa tattatgaat cacaaattga
    11281 attggcccgc gcggaaaatg acgagatact ttcccgctat cgaatcctga atggaatggg
    11341 ttgtttatta aaatatttga atttgaggtt gccggtaaat gtggttaata atgatgtgtt
    11401 ctcatcggct cagaccaaca ttttattgaa caggaaaatg gatgagattc cttatcccaa
    11461 taatacggat aatcctatgg ttctcactca tcctgttaag aacatggaga aaactcgttt
    11521 aaccaagtcg atggtagaca aaaacactac ttatcctcag caaataacac ccaagttatg
    11581 gtatatctca gcaggctctt tcagagataa aaataatgct gtagctttgg ttaaacggtt
    11641 aaatggatta ggctttactg ccttcatgag cccttataaa gatcttctgt ccgtttttgt
    11701 tggtccttat gaatatagag gtcatgcggg taatggaatg gaaaggctaa aggaattggc
    11761 tcatgttgaa ggagtcttgg ttactttcaa agagccgccc aagaaggctt agtgttatag
    11821 ccaagagatt tcagaatgtt cttttggaat ctcttcgtta tagattggtt ttatccattt
    11881 ggactggttt gatactttgc tatataacaa gtttttccac tcaagccgca cctttaatca
    11941 gtgtggaaaa aattcagaaa ttggcgcaat cctatcaggg tgacacccgg aaacggttta
    12001 ccgcatgggc gaatttaatt gactcgctta aaaaaaaacc agtaaaaata caactggaaa
    12061 aagtaaattc tttctttaat caatttaatt atgaaactga cccaataact ggtgcaagtg
    12121 atgattattg gaaatcacca gtagaattta ttgttgatgg gggaggtgat tgtgaagatt
    12181 ttgctataat aaaatacttt acattagttg cagttggtgt tccttctgac cagttaagaa
    12241 tcacttatgc tgcttctctg acactaaacc aagcacatat ggtgctgtct ttttacccaa
    12301 ctcctgaatc cgaaccacta atccttgaca gtttggaaag taaaattctg aaagcttctg
    12361 caaggcctga tttgaaacca gtttacagct ttaatgcgga aggtttatgg cttgcgaaaa
    12421 caaggggaga gagttcattg atgggggatt caaaaagttt gggcaagtgg gatgctctaa
    12481 tgaaacgtat ggaataaaaa aggtgataga ccatgacatt aactaagaaa atggctgtag
    12541 gcgtgttgat tatgttgtta ttcgttttta tcggaactta tttcatcact atgaataatg
    12601 cgcgtaattt ttttatccaa caacttgaaa gtaatgctca agatactgcg acatcattag
    12661 gcttgtcttt atctcagtct ttaatcaatc atgatgtacc taccatggat tctatggtta
    12721 aagctgtttt tgacagagga tatttttcat caattaaggt aaaagacata aaagggaaag
    12781 tgatcatttt aaagaaacaa ttgccacaag aaagcgatat accacaatgg tttgtgaact
    12841 tgataaaatg gccatcgact gagaagtcat ctttaattat ggatgggtgg atgcaagcag
    12901 gagttgtttt agtcgctagt gatccgagct atgtttacgc ctctttgtgg cgtaatgctg
    12961 tagaaatggt aaacgcttat ctgatttttg cgttagttgc tttggttgta agctattgct
    13021 ttataaaata tttattgcaa cctttaaaac gagtcacagc acaggctttg gcaatttcag
    13081 aacatgagtt tcctgtagaa acgaagattc ctaaaacccc tgaattgagg caagttacct
    13141 tggctatgaa tcaaatggtt actaaagtca aatccttatt tcaagaccag ttaaaacaaa
    13201 cagaatcact tagaacacag gtttaccaag attcattaac agggatgagt aatcgtcgtt
    13261 attttttaca gcatttggct ctgattctgg ataatgaaga tgaatttata cctggttatg
    13321 tcatgatgct ggtcatagac ggtctggatg agttaaatca gaagcaaggg tatcagcaag
    13381 gggatcaatt ggtattaact gtagccaaaa tatgtaaaag ctattggaag caatcgtctg
    13441 tcagtacatt ggcaagaatt aacgggacga cctttgcctt aattagtcat gaaagggatc
    13501 ctttggtgtt tgaaaaggaa tgtaaggagt ttgaacaaat tttgagccaa ggtatcactg
    13561 atattaaaat ttgcaaaaca tacctggggg ctgcgaatta ttttctccac caacctgtct
    13621 ccaatctgtt gtctatggta gatcaagcgg ttaaaaaggc tcgtgaaacc ggcgtatttt
    13681 attgccagaa agagcatgat acttataaat atccccagtt aatcagtggc gatgaaatta
    13741 gaaactcatt ggaacagaaa aaaataagtt tgtatgcaca ggcagttaca gatggtaaga
    13801 attgttttca taaagaggtc ttcgttcgaa ttcgcaacca ggaaggggag gagttaggcg
    13861 ctggttattt tataccagtt gctgagaaat taggattggc ctatcttatt gatcagtatg
    13921 tgctcaatga attaaccgtc atggacattg caacacatac ccattttgct cttaatattt
    13981 cagaagatac tttagcaaat aaagtcaata gcactggtta tttgcggcaa ttggaagaca
    14041 ctcctgcagc tgtcttgaga aatctttcac tggaaattaa tgaggctcac gttttgtcac
    14101 atttttcaaa ctccaagttc tttatcaagc aagccaaaaa attgggggtg acagttggaa
    14161 tagatcgtgt gggtattaag ttttctccgt tgcattattt gagtgattta aacatagatt
    14221 atttaaaact gcatgggagt ctggtgacag atattgatga aaatgaaagc aagcagtttt
    14281 tcatacatta ttttaatgaa atggctaaaa cgat
//
LOCUS       DASLOE010000070        14285 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.070, whole genome shotgun sequence.
ACCESSION   DASLOE010000070 DASLOE010000000
VERSION     DASLOE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14285
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.070"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(173..1510)
                     /locus_tag="U6309_001638"
     CDS             complement(173..1510)
                     /locus_tag="U6309_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEN5505079.1"
                     /translation="MQEWSPTTWQKYSYLQAASYADEEQLNKVVEQLSLLPPLVTSGE
                     IKHLKNEIAQAGRGNAFILQGGDCAESFNDCRSEVISNKLKIILQMSLILLYGLRKPI
                     IRIGRIAGQYAKPRSSDYETINGVTLPSYRGDIVNSPEFTATAREPNPKLLLQAYSCS
                     AMTLNFIRALLDGGFADLHHPQRWDLGFVEHSPQKNEYQHIVDSIEDALDFLNAIDGI
                     HSSSISKVDFYTSHEALHLHYEQALTRQLKDGKWYNLSTHLPWIGMRTAQTDSAHLEF
                     LRGVQNPIGIKIGPAATPEWLSEVLSIANPQKEEGRVLLYTRLGAKLIDRLLPPLIDT
                     VRKSKIPVTWSCDPMHGNTETTEDGTKTRHFDNILSELKQALEIHRSMGSYLGGVHFE
                     LTGDNVTECIGGARGLAPHDLKTAYHSLVDPRLNYEQSLEMAIQLSHQFRSEP"
     gene            1790..2389
                     /gene="grpE"
                     /locus_tag="U6309_001639"
     CDS             1790..2389
                     /gene="grpE"
                     /locus_tag="U6309_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEN5505080.1"
                     /translation="MSKQNKKDWKKFKDEHKEEHKVENEILEEETDEESQHQEPALGH
                     PSYTALEEQLTLAEQKAHENWEKSVRALAELENVRRRMEREVANAHKYGVEKLISALL
                     PVVDSLEQALQLADKNNDPSMHEGLELTMKLFLDALQKFDVEQIDPLGQTFDPQQHEA
                     MSMQPAPGAPPNSVITVFQKGYKLSDRVIRPARVIVSTK"
     gene            2534..4468
                     /gene="dnaK"
                     /locus_tag="U6309_001640"
     CDS             2534..4468
                     /gene="dnaK"
                     /locus_tag="U6309_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEN5505081.1"
                     /translation="MAKIIGIDLGTTNSCVAVMEGDKPKVIENSEGHRTTPSIVAFTD
                     DNEILVGQSAKRQSVTNPEKTLFAIKRLIGRRFDDPIVQKDIKMVPYKIMKADNGDAW
                     VRVKDQDKAPPQISAEVLRKMKKTAEDYLGEEVKEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGMDKKRGDSVIAVYDLGGGTFDISIIEIAEVDGEHQFEV
                     LATNGDTFLGGEDFDLALIEYLASEFKKDTGIDLHNDPLALQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADASGPKHLNIKLTRAKLESLVEKLVERTIEPCKTALKDAGLTVSQI
                     NEVILVGGQTRMPLVQKTVEEFFGKEPRKDVNPDEAVAVGAAIQAAVLSGEVKDILLL
                     DVTPLSLGIETMGGVMTKLIEKNTTIPTKATQVFSTADDNQTAVTVHVLQGEREQASA
                     NKSLGRFDLRDIPPAPRGVPQIEVTFDIDANGILNVSAKDKATGKAQSIVIKASSGLS
                     EEEVAAMVKDAQSHAEEDKKFKEMAELRNQADSLIHSCEKSMKDLADELSEDEKKGIE
                     TAISELKEAVQGTDKARIEDKLKVLTDASAKMAERIYAKKSSEGQAAQGQTQSQESTK
                     PAEEGVVDAEFEEVKEEDKK"
     gene            4657..5796
                     /gene="dnaJ"
                     /locus_tag="U6309_001641"
     CDS             4657..5796
                     /gene="dnaJ"
                     /locus_tag="U6309_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEN5505082.1"
                     /translation="MEQRDYYELLEVSRNASDAEIKKAYRRLAMKYHPDRNPGDTSAE
                     EKFKEIQKAYNILSDKQKRAAYDQFGHAGVDPSMGGGPGGFGGFGGFGDVFEDIFENI
                     FSGGRGHGRQSRGQRGADLQFNVQLTLEEAAIGKEVEITVPRHGTCTVCEGSGAKKGT
                     SPKTCETCQGMGQVRIQQGFFSIQQTCPTCHGEGKIISDPCASCHGQGRVRESKKINV
                     KIPAGVDNGDRVRLSGEGEAGVHGGGSGDLYVQISLKKHAIFERHENDLHCEVPISFA
                     TAALGGSIEVPTLEGRVTLKIPAETQTGKVFRLRSKGMKSVRGYGQGDLLCKVVVETP
                     VNLSREQKELLNKLQDSLENAKGTHSPKTSSWFAGVKKFFEDMKF"
     gene            5892..7004
                     /gene="carA"
                     /locus_tag="U6309_001642"
     CDS             5892..7004
                     /gene="carA"
                     /locus_tag="U6309_001642"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEN5505083.1"
                     /translation="MINKSAILALADGTVYEGLSVGATGDSVGELVFNTSMTGYQEML
                     TDPSYARQIITLTTAHVGNTGCNMEDMESTKVWAAGLVMRNCTAIPSNYRAEQSFPDW
                     LSKNGVVAISGIDTRALTLKLREKGSIGACISTNVEKPEIAVAKAKSFAGLQGADLAL
                     EVTRQTIERWHEGRGMWSLDKQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTS
                     AEEVLAMNPNGVFLSNGPGDPQACDYAIKATQVFLDNNIPLFGICLGFQILALACGGI
                     TKKMKFGHHGSNHPVVETTGKKRVFITSQNHGFAVDEDSLPNCLEVTHRSLFDNSLQG
                     IRHKTKPAFGFQGHPEASPGPHDIDILFNEFVNLMK"
     gene            7218..8366
                     /locus_tag="U6309_001643"
     CDS             7218..8366
                     /locus_tag="U6309_001643"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEN5505084.1"
                     /translation="MNEIYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACE
                     VLVKTGMVLVGGEITTKAWVDVEEITRHVIKDIGYNSSQMGFDWESCAVLSAIGKQSP
                     DIAQGVDNQQTKILGAGDQGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLP
                     WLRPDAKCQLTLKYEQGMPVEVDTVVFSTQHSPDIEHKDLVEAIREEIIKPVLPAEWL
                     NDKTRYFINPTGRFVIGGPLGDCGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSA
                     AYAARHVAKNIVAAGLADKCELQISYAIGVAEPTSIFVDTFGTGRLKNSEIIDLIHTH
                     FDLTPQGIIDQHDLLRPIYRQTATYGHYGRENFPWERLDKVAELSKAL"
     gene            8385..9710
                     /locus_tag="U6309_001644"
     CDS             8385..9710
                     /locus_tag="U6309_001644"
                     /EC_number="3.13.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEN5505085.1"
                     /translation="MELVEEGVQKINTLQDYKVADISLADWGRKEIAIAETEMPGLMA
                     LREEFSARKPLQGARIAGCLHMTIQTAVLIETLVALGAEVRWSSCNIFSTQDHAASAI
                     AAKGIPVFAWKGETEEEYWWCVEQTLSGPNGWTPNLLLDDGGDLTQVVHQKHPKLLTA
                     IKGVSEETTTGVARLYEMAKHGQLKIPAINVNNAVTKSKFDNLYGCRESLLDGLKRAT
                     DVMIAGKVALILGYGDVGKGCAQALRGQGATVLVAEIDPICALQAAMEGYRVVTLDDV
                     AEQVDIVVTATGNYHVVTHDHMKRMRNQAILCNIGHFDSEIDIQSLKQYQWENIKPQV
                     DHVIFPDGKRIIILAEGRLVNLGCATGHPSFVMSASFTNQVLAQIELFQNSSQYQNQV
                     YVLPKVLDEKVARLHLGRIGAKLTQLSNEQADYIGVDKNGPYKPDHYRY"
     gene            9834..10841
                     /locus_tag="U6309_001645"
     CDS             9834..10841
                     /locus_tag="U6309_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN5505086.1"
                     /translation="MDLVIAANYLSTLEKCLEQFGINHFLEKIGFDLIRVHDPDAYLT
                     QDEFNQVIMEAYKLSNCPELGLVFGQNLSIVNHGFLGYAAMSSPTFGTAIQTVLSYLN
                     TRTNLLSLELRQFNNENSAYIKLLPHTKEMVLSRFIIELAITHLIKMRNFLISCNDPL
                     LKIELNYDEPSYAKYYQTIFQTKIVFNTEATRFWFKAEELNYPIHFADDVSYQLAKRQ
                     LQELEKKMSLKEDMASKIKSILINQNLNHVSMDEIASRLCMTSRTLRRHLQRLDVTYQ
                     ELLDEVREQKAKTFLLNNNISMTEISFLLGFQDTSNFSKAFKRWTGVTPTEYREKHEV
                     Q"
     gene            10973..12661
                     /locus_tag="U6309_001646"
     CDS             10973..12661
                     /locus_tag="U6309_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEN5505087.1"
                     /translation="MGLKSGILGLSLLSLACGSFAMTETETIRVIIKYKQQPATIASL
                     KSQVIKSVQIPVKEMKPMANGAYSLIFDTSNSHAIVKAKEEDVTAKVLERLRKNPNVL
                     YAVKDRIGHFKPLPDPVINQELSQLLSHESQWDEFERPAGIRLESKPGLRDGAWAYTT
                     GKSRKPIVVAVLDTGIALNDSLVNNLVKDNEGNVFGWNFAANNNDLSDETRSYHGTHV
                     AGTIAGYGDVMNGVGEDLKILTVKIPAENGMFYESAVVNSIYWAVGGDVPGVPKNIHP
                     AKVLNMSFGVDLEPGKEIDYCDEALQEALFFARKKGAVVAVAAGNDNVWEHYNAPAVC
                     NGTIKVASTGPEGLRSYFSNYGPSITLAAPGGDKRYGTSGGILSTVNPGGGYNSSGFD
                     FYQGTSMASPHVAGVAGLIFAVSNKTLKPEQVEQILYVTTHEFGQSNDANKSCVGKKP
                     CGHGILDADNAVQAALNAYDVLFSAPKMTDLVTHECGENRLKPGKNIINDSTATWIQA
                     KSSCETGASYQNPRIEKAKDGKIYAYYGSVSYELDQSAYKECRVVGYDGVGCYL"
     gene            complement(12815..13096)
                     /locus_tag="U6309_001647"
     CDS             complement(12815..13096)
                     /locus_tag="U6309_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505088.1"
                     /translation="MAETIDDITIAFNENGVETTRELDKQILSKGAWTTIMFKYQDWD
                     NTKNDYGPVKYSIRRYQKRNNQYWQKSKFNISSTEQAKKIVDILNNWLE"
     gene            complement(13110..13724)
                     /locus_tag="U6309_001648"
     CDS             complement(13110..13724)
                     /locus_tag="U6309_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HEN5505089.1"
                     /translation="MEKAMKSILLMSLLLLIMPFRLMSAQSLYCPQNHGYINIGMSME
                     QVIAACGQPLSKQESNQPILQKIPVQQLIYNNLGNSDGLYSGSLNVPEGTAFYGVWNI
                     PTGSSGVQLEVDILNNKVQDIRVNGGNTNAFSLCGNASIQIGDPATKVYGACGSPNLV
                     NNTYINQVVPTETKPQIWIYKPGPYQPAFSLTFVDGKLQSIGNN"
     gene            complement(13772..>14285)
                     /locus_tag="U6309_001649"
     CDS             complement(13772..>14285)
                     /locus_tag="U6309_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEN5505090.1"
                     /translation="SLWLRIAIRFFRISAINPFSQLIHTITDPLVKPLYWIIKKPLQP
                     GQKYDWIAFGVLILVEFFKIISLSLLMYHAIIPFLFIFLYILVDLIIQPCEILFFAVL
                     IRVIMSYVNPKWQHPLGDFLRIITTPILRLGRIIVPDISGFDFSPFIIMVILKIITLF
                     LSASLPWKLL"
BASE COUNT         4424 a         2474 c         3149 g         4238 t
ORIGIN      
        1 gcaggcggga atctaactaa aaatcaatct atgtgctagc tttatggata gatttccgcc
       61 cgcgcgggaa agacagggcg cttaatattc caattacata ccctgtgaac agttacaaac
      121 aatcggcttt tggcgtagat actggccttc attcaagacg ttatgagttt cattaaggtt
      181 cacttctgaa ttgatggctt aattggatgg ccatttccag agattgttca tagtttaatc
      241 ttggatcaac caggctgtga taggcagttt tgaggtcatg gggagctaat ccacgagcgc
      301 ctccgataca ctctgttaca ttgtcaccag ttagctcaaa atggacacct ccgaggtagc
      361 tacccatgct gcgatgaatt tccaaagctt gttttaattc cgataaaatg ttatcgaagt
      421 gacgtgtttt agtaccgtct tctgttgttt cggtattgcc atgcataggg tcacatgacc
      481 acgtgactgg aattttgctt ttccttactg tgtcaatcag tggaggtaac aaccggtcga
      541 taagttttgc tcccaagcga gtataaagta aaactcgtcc ttcttctttt tgcggattgg
      601 ctatgcttaa tacttctgat aaccattcag gtgtagctgc tgggcctatc ttaatgccta
      661 ttggattttg tacccctcgt agaaactcaa gatgtgcact gtccgtttgt gccgtacgca
      721 ttccaatcca gggtaaatga gttgaaagat tgtaccattt tccatctttc aactgtcttg
      781 ttaatgcttg ctcataatgt aaatgcaacg cttcatgaga ggtgtaaaaa tcaactttac
      841 ttatgctact cgaatgtatt ccatctatgg cattcaaaaa atccaaggcg tcttcaatag
      901 agtctacaat atgttggtat tcgttcttct gaggggagtg ttcaacaaaa cctaaatccc
      961 agcgttgagg gtgatgcagg tcagcaaaac cgccatccag cagcgctcgg ataaaattta
     1021 aagtcatggc agaacaactg tatgcctgaa gtaataattt aggattaggc tccctggcag
     1081 tagcagtaaa ttcaggtgaa ttaacgatat ctccacgata actgggtaaa gtaactccat
     1141 ttatggtttc gtaatctgag gagcgtggtt ttgcatattg tccagctatt cttccgatac
     1201 gaataattgg tttacgcaag ccatataata agattaggct catttgaaga atgattttta
     1261 atttgttgct tatgacttct gaacgacagt cattaaaaga ttcagcacaa tcaccacctt
     1321 gcagaataaa agcattaccc cggccagcct gagcaatttc attttttaaa tgtttgattt
     1381 caccgctggt aaccaatggg ggtagcaaac ttaattgttc taccacttta tttaattgct
     1441 cttcatcagc gtatgaagct gcctgcaaat acgaatattt ctgccatgta gttggcgacc
     1501 attcttgcat aatttaccca agttattttt gctttagttg tcgtttagac ttaatttaac
     1561 tcaaaggatt attaattact ttgatttttt tacctaaatg agaattaaga cagatttttt
     1621 agctacctaa gtatggaata aatggacaat tactgcaagc catgatttga aaagtttcaa
     1681 tcagttaatg acctgaattt taaaccaaga tttaaaatac taacaggggc ttgaaaattg
     1741 caaatacaat ccccatttgt aggaatcgag ttagtcagga gcgattttga tgagtaaaca
     1801 aaataaaaaa gattggaaaa aatttaaaga cgaacacaaa gaagaacaca aggtagagaa
     1861 tgagattctg gaagaagaaa ctgatgaaga atcacaacat caagaacctg ctttaggaca
     1921 tcccagctat actgccttgg aagagcaact gacattggcg gaacaaaaag cacatgagaa
     1981 ttgggaaaaa tctgtacgcg cccttgctga attggagaac gttcgtcgtc gtatggaacg
     2041 agaagttgcc aatgcccata aatatggcgt agaaaaactg atttcagctt tattgccagt
     2101 agtggacagt cttgagcaag cattacaatt agcggacaag aacaatgatc cgtctatgca
     2161 tgaagggctt gagttaacca tgaaactctt tcttgacgca ttacagaagt ttgatgtgga
     2221 acaaattgat cctcttggac agacatttga ccctcaacaa catgaggcaa tgtcaatgca
     2281 acctgcccca ggagcgccac cgaattctgt aattactgta ttccaaaaag gttacaaatt
     2341 gagcgatcga gtaattcgac cagctcgggt gatagtttcc accaaataat ctggtgtttc
     2401 agtggaggta tttttaaaca aatttttaga cgcagtgtat tcagcttttt ttagggggat
     2461 agcttgaaat cgtcatgatg caccccatat gtggaatatt gcatgagaaa atattaattt
     2521 ggagcaaaat agaatggcta aaataatagg tatagacctg ggtactacga actcttgtgt
     2581 tgctgtcatg gaaggtgaca aaccgaaggt aattgaaaat agtgaaggtc acagaacaac
     2641 tccttctatc gtagcattta ccgatgacaa tgaaatattg gtaggccaat cagccaagcg
     2701 tcaatccgta actaacccag agaaaacact gttcgcgatt aaacgattaa ttgggcgtcg
     2761 atttgatgat cctattgtgc aaaaagatat taagatggtt ccctacaaaa ttatgaaagc
     2821 ggacaatggg gatgcttggg ttagagtcaa ggatcaagat aaggcaccac cacaaatttc
     2881 tgctgaagtg ctaagaaaaa tgaaaaaaac agcagaagat tatttgggtg aagaagtaaa
     2941 agaagcagtg attactgtac ctgcctattt caacgattcg caacgccagg caaccaaaga
     3001 tgctggtcgt attgctggcc tggaggtaaa acgaatcatt aatgagccta ccgctgctgc
     3061 attggcttat ggtatggata aaaaacgcgg tgactctgtt attgcggtat atgatttagg
     3121 gggaggaact tttgatattt caattattga aattgccgaa gtggatggtg aacatcaatt
     3181 tgaagtactt gcaaccaatg gtgatacctt cctcggtgga gaagattttg atttggcttt
     3241 aattgagtat ttagcttctg agttcaagaa agacactggt attgatttgc ataatgatcc
     3301 tctggctctt caacgcttga aagaggctgc cgagaaagca aaaattgaat tgtcatctgc
     3361 acaacaaacc gatgtcaatt tgccttatat tacagcagat gcctctggac caaaacattt
     3421 aaatatcaaa ttgactcgcg caaaactgga atctttagtg gagaaactgg ttgagagaac
     3481 tattgagcca tgtaaaacag ctttaaaaga cgcagggctt actgtctcac aaatcaacga
     3541 agttattctg gtcggtgggc aaactcgtat gcctctggta caaaaaactg tggaagaatt
     3601 ctttggcaaa gaacctcgta aggatgtaaa ccctgatgag gctgtggcag taggtgctgc
     3661 aattcaggca gcagtattgt ccggtgaagt gaaagacatt cttttactcg atgtcactcc
     3721 tttgtctctc ggtatagaaa ctatgggtgg tgtaatgacc aaactcattg aaaaaaacac
     3781 aactattcct acaaaggcta cacaagtgtt ttcaacagcc gatgataatc agacagcagt
     3841 gactgtgcat gtattgcagg gtgaaagaga acaggcctca gcaaacaagt cattaggacg
     3901 gtttgatttg cgtgacatcc ctcctgctcc tcgtggtgtt cctcaaattg aagtaacttt
     3961 tgatattgat gccaatggta tcttgaatgt ttctgccaaa gacaaggcaa ctggcaaggc
     4021 acaatccatt gtcatcaaag cttccagtgg tttaagtgaa gaagaggttg ctgctatggt
     4081 gaaggatgcc caatcgcatg ctgaagaaga taagaaattc aaggaaatgg cagaactgcg
     4141 caatcaggca gatagtttga ttcatagctg tgaaaaatcc atgaaagatt tggctgatga
     4201 gttgtctgag gatgagaaaa aaggcatcga aacagcaatt tctgagctga aagaggcggt
     4261 gcaaggaact gacaaagctc gtattgaaga caagctgaaa gtattgacag acgcttccgc
     4321 aaaaatggct gaacgtattt atgctaaaaa atcgtctgaa ggtcaggcag ctcaggggca
     4381 aacacaatct caggaatcta caaaacctgc ggaagaggga gttgttgatg ctgagtttga
     4441 agaagttaaa gaagaggata aaaagtaatt taatattact ctcgccctct gatttaagag
     4501 ggcggaagaa ggattcatcg ggttataata aataaatttt gggtcggggc gacattatcg
     4561 tcccgtttgt ctattgaata gcacttaagc taatttagag tcttcatacg actgagagaa
     4621 ttataattaa gtgatcgtta taaattgtga gcagttatgg aacagcggga ttattatgaa
     4681 cttttagaag taagtcgaaa tgcgagtgat gcagagatta aaaaagcata tcgcaggctg
     4741 gcaatgaaat accatcccga tcggaatcca ggtgatacct ctgccgaaga aaaatttaaa
     4801 gaaattcaaa aagcctataa tatcttgtcc gataagcaaa aacgcgccgc ttatgatcaa
     4861 ttcggccatg ccggagtgga tccttcaatg ggtggcggcc caggtggttt tggcggattt
     4921 ggaggatttg gtgacgtttt tgaagatatt tttgagaata ttttttcagg ggggcgtggg
     4981 catgggcgtc aatcacgtgg acaaagaggc gctgatttgc aatttaatgt gcaattgacc
     5041 cttgaagaag cagctatagg aaaagaagtt gaaattaccg ttccaagaca tggtacttgt
     5101 actgtttgtg agggatcggg ggctaaaaaa ggcacaagcc caaaaacttg tgaaacatgc
     5161 cagggcatgg ggcaagtcag aattcaacaa ggtttttttt ccatacaaca aacctgtccc
     5221 acttgtcatg gcgagggaaa aataatttct gatccttgtg ccagttgtca tggtcaagga
     5281 agagttagag aaagtaaaaa aataaatgtc aaaatcccgg ctggagtcga taatggtgat
     5341 cgagtccgat taagcggaga aggtgaagca ggggtgcatg gtggtggttc aggagattta
     5401 tatgttcaaa ttagcttgaa aaaacatgct atttttgagc gccatgaaaa tgatttgcat
     5461 tgtgaagtac ctattagttt tgcaactgcg gcgttaggag gctcaatcga ggtacccact
     5521 ttggaaggcc gagtcacttt aaaaattcca gcggaaactc aaacaggcaa agtgttccgc
     5581 ttgagaagta aagggatgaa atctgtcaga ggatatggac aaggcgattt gctatgcaag
     5641 gtagttgtag aaacaccggt caatttatct cgagagcaaa aagaactgtt aaataagtta
     5701 caggattcat tagagaatgc taagggaacg cactcaccta aaacaagctc ctggtttgct
     5761 ggtgtgaaaa aattcttcga agatatgaaa ttttgactta attaagttac aattggaaga
     5821 tttgtcgttt tcagcatgga ttgcctattt tacgctggat aagcatactt tgaacataat
     5881 tgagtatatt catgattaat aaatcagcaa ttttagcttt agctgatggc actgtttatg
     5941 agggcctgtc agtaggggcc actggtgatt ctgttggtga gttggttttt aacacgtcaa
     6001 tgactggcta tcaggaaatg cttactgatc cttcgtatgc cagacaaata attaccttaa
     6061 ctactgctca tgtgggaaat acgggttgta acatggagga catggagtcc acaaaagtct
     6121 gggcagccgg cctcgtcatg agaaactgca ctgcaatccc tagcaattac agagctgaac
     6181 aatccttccc agactggtta agtaaaaatg gagtagttgc aatttctggc attgatacgc
     6241 gcgcgcttac cctgaaattg cgagagaagg gttctatcgg agcttgtatt agcaccaatg
     6301 tggagaaacc tgaaattgct gtggcaaagg ctaaatcctt tgcagggctt cagggtgcgg
     6361 atttggcttt agaggttaca agacaaacga tagagcgttg gcatgaaggt cggggaatgt
     6421 ggagtctaga caaacagcct caacagcttc atgttgttgc ctatgatttt ggtgtgaaac
     6481 ataatatttt acgcatttta catgataagg gatgccatat tactatagtc cctgctaaaa
     6541 cttcagcaga agaggtttta gccatgaatc ctaatggtgt ctttttatcc aatggaccgg
     6601 gtgatcccca agcatgcgat tatgctatca aagcaacaca ggtattttta gacaataata
     6661 ttcctttatt tggtatatgc cttgggtttc agattttggc tttggcatgt ggtggtatta
     6721 ctaaaaaaat gaaatttgga catcatggtt ccaatcatcc tgtagttgaa acaactggca
     6781 aaaaacgtgt ttttataaca agtcaaaatc atggttttgc tgtagatgag gattcattgc
     6841 ctaattgcct ggaagttacg catcgttcat tatttgataa cagtttacag ggaataaggc
     6901 acaaaacaaa accggcattt ggttttcaag gccatccaga agcaagcccc gggccacatg
     6961 atatagatat tttatttaat gaatttgtaa atctgatgaa ataaattttt agcttcagaa
     7021 ttattatttt ggcaagagtg gcctgtttta ttttaagcct gcttacaatg attgtaatac
     7081 ccctaaatgc tatccatggc tattaaagat tagtaaaatg acatggatta ccaaaacaat
     7141 gactttaact tgttttgtgt gtcaagcaga gataaagaat tagggatact aaaaatagaa
     7201 aatacagagg gtatgtaatg aatgaaatct atgtttttac ctcagagtct gtttccgagg
     7261 gccatcctga taagatagct gatcaaattt cagatgccat tttagatgct attttagccc
     7321 aagatccaaa agcacgagtg gcttgtgagg tattggttaa aaccggtatg gttttagttg
     7381 gtggagaaat tacgacgaag gcttgggttg atgttgaaga aataactcgt catgttatta
     7441 aggacattgg ctataatagt tcccagatgg ggtttgattg ggaatcttgt gctgtgcttt
     7501 cagcgattgg taagcaatca ccagacatag ctcaaggtgt cgataatcag caaactaaaa
     7561 ttctgggtgc tggcgatcag ggacttatgt ttggttatgc cagccgtgaa acagatgttt
     7621 ttatgcctgc ccctattgcc tatgcacatc gtttgatgga gaaattagct aaagctcgta
     7681 aatcaggcca gttaccctgg ttaagacctg atgcaaaatg tcaacttact ttgaaatatg
     7741 aacaagggat gccggttgaa gtggatactg ttgttttttc cacgcagcac tcacctgata
     7801 ttgaacataa agatttggtt gaggcgatcc gtgaagaaat tattaaacca gttcttcctg
     7861 ctgaatggct caatgataag acccggtatt tcattaatcc aacaggacgc ttcgtaattg
     7921 gtggtccttt gggcgattgt ggtttaaccg gccgtaaaat cattgttgat acttatggtg
     7981 gtatggcaag acatggcgga ggctgttttt caggtaaaga tccttctaag gtagatcgct
     8041 ccgcagcata cgccgcaaga catgttgcca agaatattgt ggcggctggt ttggcagata
     8101 aatgcgagtt acagatttcc tatgctattg gtgttgctga acctacatct atttttgttg
     8161 atacttttgg tactggacga ctaaaaaaca gcgaaataat tgatttaatt catacgcatt
     8221 ttgatttaac accgcaaggt attattgatc aacacgattt gcttcgtcct atttataggc
     8281 agacggcaac ttatgggcac tatggcaggg agaattttcc atgggagcga ttggacaaag
     8341 tagctgaatt aagcaaggca ttataatttt ttaaggatac tctaatggaa ctggtagaag
     8401 aaggagtaca gaaaatcaat acattacaag attataaagt agcagatatt tcattagcgg
     8461 actgggggcg aaaagaaata gcgatagctg aaaccgaaat gccaggttta atggctttac
     8521 gcgaggaatt ttctgcgaga aaaccacttc aaggcgctcg gattgcaggt tgtctgcata
     8581 tgacgattca aaccgcagta ttgattgaga ccctggttgc attgggtgct gaagtacgct
     8641 ggtcttcctg caatattttt tctactcagg atcatgcagc ttccgctatt gctgcaaaag
     8701 gaattcctgt ctttgcctgg aagggcgaaa cagaagaaga atattggtgg tgtgttgagc
     8761 aaactttaag tggccccaat ggttggacac ctaatttatt attagatgat ggtggggatc
     8821 tgacacaagt tgttcaccag aaacatccta aattactgac tgcaattaag ggagtatctg
     8881 aggaaaccac tacaggtgta gctaggttgt acgaaatggc gaaacatgga caattaaaaa
     8941 ttccagccat taacgttaat aatgcggtga ccaaatccaa atttgataat ttgtatggat
     9001 gtcgggaatc cctgcttgat gggttaaaac gtgcaacaga tgttatgata gctggtaagg
     9061 ttgccttgat tctaggctat ggtgacgtag ggaaaggttg cgctcaggcc ttgcgtggac
     9121 agggagctac agtacttgtc gcagaaattg atcctatttg cgcacttcag gcagcaatgg
     9181 aaggctacag agtcgtaact ctagatgatg ttgctgaaca agtggatatc gtagttacag
     9241 ctactggcaa ttaccacgtt gtaactcacg atcacatgaa acgcatgcgt aatcaggcga
     9301 tattgtgcaa cataggccat tttgattcgg aaattgatat tcaaagctta aaacagtatc
     9361 aatgggaaaa tatcaagccg caggttgatc atgtgatttt tcctgatgga aagcggatta
     9421 ttatcctggc tgaagggcgt ttagtgaatt tgggctgcgc tacaggccat ccgagttttg
     9481 ttatgtctgc ttcctttacc aatcaagtat tggcgcagat tgagctgttc caaaactcta
     9541 gccaatacca gaatcaagta tatgtacttc caaaagtttt ggatgaaaaa gtagcccgtt
     9601 tgcatttggg gcgtattggt gcaaaattaa ctcaattaag taatgagcag gctgattata
     9661 tcggcgttga taaaaatgga ccttacaagc cggatcatta tcgttattaa actaatttta
     9721 ttcaagattt agtcaagaca actaaatctt gaattttttt acattattgg atcttatact
     9781 ctaatataga ttagaattaa ttcacttctc acttaaaaaa gaaaagcctg ttgatggatc
     9841 tggttatagc ggcaaattat ctttctactc tggaaaaatg tcttgaacaa tttgggatta
     9901 accatttttt agaaaaaatt ggctttgatc tgataagagt acatgatcct gatgcctact
     9961 taactcagga tgaatttaac caggttatta tggaagctta taaattaagc aattgcccgg
    10021 aattgggttt ggtttttggt caaaacttat ctatagtcaa tcatggtttt cttgggtatg
    10081 cagcaatgag cagtcctaca tttggaactg ctatacagac tgtgctcagt taccttaata
    10141 cacgaaccaa tttgttgtct ctggagttac gccagtttaa taatgaaaac agtgcttata
    10201 tcaagttatt accacacaca aaagaaatgg ttttatcacg ttttataatt gaattggcaa
    10261 ttactcatct tattaaaatg agaaattttt tgattagttg taatgacccg ttgctcaaga
    10321 ttgagttaaa ttacgatgaa ccttcttatg caaaatatta tcaaacaatt tttcagacta
    10381 aaattgtttt taatacagag gctactcgct tttggtttaa agcggaggaa ctgaattatc
    10441 ccatccattt tgctgacgat gtcagttatc agctggcaaa aaggcaattg caagaacttg
    10501 aaaaaaaaat gtcattaaaa gaggatatgg caagcaagat taaatctatt ttaatcaatc
    10561 agaatttaaa tcatgtcagc atggatgaga tagccagtcg actatgtatg acttctcgta
    10621 ctctaagacg tcatttgcag agattggatg tgacttatca agagttgctt gatgaggtaa
    10681 gagaacaaaa agcaaagaca tttttgctca ataataatat ttccatgacc gagatcagtt
    10741 ttttattggg ttttcaagat acatccaatt tttcaaaagc attcaaacga tggacaggag
    10801 ttactcccac agaatatagg gaaaaacatg aagtccaatg attgctccat tttaatttaa
    10861 tctcatggct tattgattct tgagttgata tttattctcc tgcatactga ggtggtttta
    10921 tttttaaaaa ttggtcaagg ctacggattg actttaaaaa ggagattgaa gtatgggttt
    10981 gaaatcagga atattgggat taagtctttt atcgcttgct tgtggcagtt ttgctatgac
    11041 tgaaactgaa acaatcaggg ttatcattaa atataaacag caacccgcca ctattgcttc
    11101 actaaaatca caagtcatta aatctgttca aataccagtt aaagaaatga agcctatggc
    11161 caatggcgct tattcgctta tttttgatac atcgaattcc catgctattg tcaaggctaa
    11221 agaagaagat gttacagcca aagttttaga acgtttgcgc aaaaatccca atgtacttta
    11281 tgcagttaaa gacagaattg gacattttaa gcctctccct gatccagtaa taaaccagga
    11341 gttaagtcaa ttgttatccc atgagagtca atgggatgaa ttcgaaaggc ctgcgggaat
    11401 tcgtctggaa tccaagcctg gtttaagaga tggcgcatgg gcatatacta caggaaagtc
    11461 caggaagccg atagttgttg cggttttaga tactgggatt gcattgaatg acagtttggt
    11521 gaataacctt gtaaaagata atgaaggtaa tgtatttgga tggaattttg ccgcgaataa
    11581 taatgattta tctgatgaaa caagatctta ccatggcacc catgttgccg gtaccattgc
    11641 cggttatggc gatgtcatga acggagttgg tgaggattta aaaattctga ctgttaaaat
    11701 tcctgccgag aatggtatgt tttatgaaag tgctgtggtg aatagtatct attgggctgt
    11761 tggaggtgat gttccgggag ttcctaaaaa tatacatcca gccaaggtat taaatatgag
    11821 ttttggtgtc gatttagagc ctggaaaaga aattgattat tgcgacgaag ctttacaaga
    11881 ggctttattc tttgctcgca aaaaaggggc tgtagtcgcg gttgctgccg gaaatgataa
    11941 tgtttgggaa cactataatg cgcctgctgt gtgcaatgga acgatcaagg ttgcttccac
    12001 cggacccgaa ggcttgcgtt cgtatttttc aaattatggg cccagtataa cccttgcagc
    12061 acctggaggt gataagcgtt atggcacttc cggaggtatt ttatctaccg taaacccggg
    12121 aggtggatac aattcatcag gttttgattt ttatcaaggg acgagcatgg cttcgcctca
    12181 tgtagccggg gtagcaggtt taatttttgc tgtcagtaat aagactttga aacctgaaca
    12241 agtagaacaa attttatatg ttactacgca tgaatttgga caaagcaatg acgccaataa
    12301 atcttgtgtc ggcaaaaaac cttgtggaca tggaatattg gatgcggata atgctgtgca
    12361 ggcagcatta aatgcttatg atgtcttatt ctcagcgcct aaaatgactg atctggtaac
    12421 gcatgaatgt ggcgagaata gattaaaacc tggtaaaaat atcattaatg acagtaccgc
    12481 gacctggatt caagctaagt caagctgtga aactggtgca agctaccaga atccgcgtat
    12541 tgaaaaagcc aaggatggaa agatttatgc ttattatggc tccgtgagct atgaattgga
    12601 tcaatcggct tataaagaat gtcgtgtcgt tggatatgat ggcgtaggct gttatttata
    12661 atatcaaatg aaaatcgatt ctcagttttc agatgttttt gatgagtgaa gtattcagag
    12721 ccaatttact ggttatttat ggaatccttc tctcatcaaa ttaaaaacta tgcatttgct
    12781 catgcattaa aattttaata aagatatctg aaaattactc aagccaatta tttaatatat
    12841 caacaatttt tttggcttgt tcagtactcg aaatattgaa tttggatttt tgccaatatt
    12901 gattgttacg tttctgataa cgccgtatag aatattttac aggaccataa tcattttttg
    12961 tgttatccca atcctggtat ttaaacataa tagtagtcca cgctcctttg gataggattt
    13021 gtttatcgag ttccctggtt gtttccactc cattttcgtt aaaagcaatt gttatatcat
    13081 ctatggtttc agccatttgt tatacttcct caattatttc cgatggattg taatttgccg
    13141 tcgacaaaag tcaaggaaaa ggctggttga tatgggcctg gtttataaat ccagatttgt
    13201 ggttttgtct ctgtgggaac cacttggtta atgtacgtgt tattgaccag gtttggactg
    13261 ccgcaagcgc cataaacttt agtggcagga tctccgattt gaatgcttgc gttgccacac
    13321 aaggaaaaag cgttagtatt gccgccattc actcgaatat cctggacttt attattgaga
    13381 atatcaactt ccaattgcac accactacta cctgtaggta tattccatac gccataaaaa
    13441 gcagtacctt ccggcacatt taatgatcct gaatacaggc catcagagtt tcccagatta
    13501 ttataaatta gttgttgcac agggattttc tgcaaaatag gctgattgga ctcttgttta
    13561 ctcagtggtt gcccgcacgc agcaatgacc tgctccatgg acataccgat attgatataa
    13621 ccatgattct gagggcaata caaagattgt gcgctcatta aacggaatgg cataattagc
    13681 aataataaac tcataagtaa tatcgatttc atagcttttt ccattaatcc gtatcaacta
    13741 atagtatagt tctgtttgtt tatttttgct tttataataa cttccagggc aaagaagcgc
    13801 ttagaaacag ggtaataatt tttaatatca ccatgataat aaagggtgag aaatcaaaac
    13861 ctgaaatgtc cgggactata attctgccta gtcgtaaaat tggtgttgtt attatgcgca
    13921 agaaatcacc aagcggatgt tgccatttgg gattaacata actcattatt actcgaatca
    13981 gaacagcaaa aaataatatt tcgcaaggtt gaattatcaa atcaacaagt atgtagagaa
    14041 aaataaagag aaatggaatg attgcgtgat acatcagtaa actcagtgaa attattttaa
    14101 aaaattccac caggattaaa accccaaatg ctatccagtc gtatttttgt cctggttgca
    14161 atggcttttt tataatccaa tacaagggtt tcactagagg atcagtaatg gtatggatta
    14221 attgagagaa tggattaata gcactgatac gaaaaaatcg tatagcaatt ctaagccaca
    14281 aactg
//
LOCUS       DASLOE010000071        14114 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.071, whole genome shotgun sequence.
ACCESSION   DASLOE010000071 DASLOE010000000
VERSION     DASLOE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 14114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14114
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.071"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            182..1414
                     /locus_tag="U6309_001650"
     CDS             182..1414
                     /locus_tag="U6309_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5505091.1"
                     /translation="MSVSRVTAWFVWVIVSIFYAYQYVLRVMPNIMLDDITQQFHIDA
                     IIFGQFSGIYYIGYSLMHLPIGIMLDRFGPRKVMTGCIMLTVIGLLPILFAENWVYPV
                     IGRALIGIGSSAAILGTFKIIRMTFKEKHFPRMLSFSVMIGLVGAVYGGGPVSYMCQL
                     MGYKMVVEIFVGIGIVLAGITYLIVPDAQPSRQDSVISNIRMVFGNSKVILLCCFAGL
                     MLGPLEGFSDVWGSGFLKMAYSLNANAANYLPSMIYIGMCFGAPILSLIAEKTGYYLG
                     TIIASGVVMFLVFAMLVGGVLTIPSITISFILVGICCSYQILAIYKVSTYVPDNLAGL
                     TTAIANMIIMIFGYGFHSVIGYVVNMYGGTHVARAFVYGVGVIPLTLAVGVVGFLFLA
                     YQEKNFTPDASFASPELR"
     gene            1469..1783
                     /locus_tag="U6309_001651"
     CDS             1469..1783
                     /locus_tag="U6309_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HEN5505092.1"
                     /translation="MYMLYFHVPETHLDEVKNAVFAAGAGYVDNYNHCAWQTLGEGQF
                     MPLPGSQAFIGEINQLEKVPEYKVETICARERIKEVVAALKKAHPYESPSYQVVRIET
                     EF"
     gene            1974..3527
                     /locus_tag="U6309_001652"
     CDS             1974..3527
                     /locus_tag="U6309_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEN5505093.1"
                     /translation="MLKILALLSCLILSSCLVGPNFKEPKKDAANYWLTSSPAIKQSA
                     FQNANWWKVFNDPTLTSLIDNGYHNNLSLQMAGVRVLQARAQLAQSVGELYPQQQAMV
                     GDYNYNRIGGNQLQGLLPPDFLTASLGFSASWELDFWGKYRRAIQSNDALFLASLAAY
                     DSALVTLTSDIASTFIKIRTSEELIKVTKTNIQVQAMSLKIAKDRYYGGQTSLLDVEQ
                     AATELAETQSKLPQYVSDLQHQKDILAVLLGTTPDKVDDLLKKNKGIPKALAVVEVGI
                     PKEVIARRPDVYQARMEAIAQSAAIGAVKANLYPSLSLVGTFVFASNNIGNSTISDIF
                     NWSNRNIVAGPSLAWSLLNYGQITNAVRAQDAAFQQALLNYLNLVLKVQQEVQDNITR
                     YVEGQKTVSSLKTANAAAIESTRLALVRYKEGEANYTTVLNAEQQQLRVQTSLVNATG
                     ELALSLVGLYRSLGGGWQIRGKNDIVSNQIKSEMAARTNWGSLLKQENHLPPDSNGQR
                     MKQLYLPTW"
     gene            3533..4687
                     /locus_tag="U6309_001653"
     CDS             3533..4687
                     /locus_tag="U6309_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEN5505094.1"
                     /translation="MNSQNKQNLVKTVAIVFFGIVFVYLVYDHFTRAQVATPAPPDVI
                     VQKPKLMKMGQYVTQTGNTVAFQSVDLVARIEGYLDAIKFTDGAFVKKNQELFIIEPE
                     PYLEQLRAAEAKVAAQKANYAYAKSEYARQQRMYKQNATSLNSVEKWKAKVEEAQAEV
                     DKSEADATNAAITYGYTHVSAPFDGRMGRHLVDVGNLVGHGAATDLATIEQIDPIYVY
                     FNLNELDLIRIRNAARKRGYKPMELVKIPVEVALQDEKGFPHKGTLDFVNTGLNASTG
                     TMEFRALLPNKGYPLVPGLFVQVRVPISEPSPKLTIPDTSVQYDQIGPYVLVVDKDNY
                     VLTKRVTLGGLENGVRAITKGLDAQDSVIVSGLQNATPNHRVTPKTEQPE"
     gene            4747..7899
                     /locus_tag="U6309_001654"
     CDS             4747..7899
                     /locus_tag="U6309_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEN5505095.1"
                     /translation="MISKFFIERPVLANVIALFIVFIGIIAIAVLPVSQYPAIVPPTI
                     QVTTTYPGADAKTLINTVALPIEQQVNGVEDMLYMQSTSTSGGTYTLIVTFAIGTDLN
                     YAQVLVQNRVQAAMAQLPESVQKQGVIVQQKSTAILQFITLTSKNNEYDGVFLDSYAT
                     INMQDELSRLPGVGNVVVFGSGSYAMRVWLDPKKMLAFSLNPSDVLNAISYQNKEVSA
                     GQLGAPPTVGKQAYQFTVNVPGQLSDPKEFENIIIKTIDTPPDQNSSASSSAQVVRIR
                     DVGRVELGSESYNQLANLNGKPTAAIGIFQLPGANALDVAQEVRKAVAKMAKQFPPGL
                     EYSIPFDTTVFVKASIEEVYKTLFEAGILVLIVIVVFLQNFRASLVPATTVPVTIIGT
                     FFAMLLLGYSINLLTLFALVLAIGIVVDDAIVIVEGVTQHIEKGLSPKESAILAMKEL
                     FGPIIGITLVLMAVFVPAGFMPGLTGSMYAQFALVIAATAFISAINAMTLKPTQCALW
                     LKAPDTSKPKNIFFQAFDRIYNPIESAYVKFIDRLVHRSGKVCLIGIILVACAIYGLT
                     RIPTGFIPMEDQGYLMLSIQLPDGASLGRTDEVVSRLAKKASEVGGVDNVIAIDGISL
                     LDNNSLLSNAGVIYVIFKDWSVRGKSENLRALYTKFNAIAKETLDAKVLVVVPPPIQG
                     LGMSGGFQMQVELQDGTFDYRKLQQATDQMINTGRQYPQLQNLMTTFRASVPQVAAPI
                     NRTKAESLGVRVADAFDTLQTYLGSSYVNLFTKFGQVFPVYVQADASSRVSSEDLRNY
                     YVRNQSGSMVPLGTLTDVGPAVGPSIISLYNLYPSSNINGVAARGYSSGQGIQVMEKL
                     AKEQLPPGISYEWTSTAYQEKVAGNLSYFIFALSLVLVYLILSGQYENWLIPSAIILS
                     VPLTLVGTVLALGSLGMDNNMYTQIGLLLLIALATKNAILIVEVAREQREIHNKSVLE
                     AAVIGAKTRFRPILMTSFAFIMGVMPLVFATGAGANSRRSIGIAVSSGMLASTCLAVV
                     FVPVFYVLLQTWQDKRKAKH"
     gene            complement(8265..11018)
                     /gene="sidC"
                     /locus_tag="U6309_001655"
     CDS             complement(8265..11018)
                     /gene="sidC"
                     /locus_tag="U6309_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidC"
                     /protein_id="HEN5505096.1"
                     /translation="MVINMVDVIKFKEPERCDYLYVDENNKVHILLPIVGGDEIGLDN
                     TCQTAVELITFFYGSAHSGVTKYSAEHQLSEYKRQLEEDIKAINSQKKISPHAYDDLL
                     KEKIERLQQIEKYIELIQVLKKQYDEQNDIRQLRTGGIPQLPSGVKEIIKSSENAFAV
                     RLSPYDNDKFTRFDDPLFNVKRNISKYDTPSRQAPIPIYEGLGYRLRSTLFPEDKTPT
                     PINKKSLRDKVKSTVLSHYKDEDRIDGEKKDEKLNELITNLQNELVKELVKSDPQYSK
                     LSLSKDPRGKEINYDYLVNSLMLVDNDSEIGDWIDTILDATVDSTVWVAQASSPFYDG
                     AKEISSDRDADKISIRVQYLLAEANIYCKTNKLSDANFGEFFDKEPHATEIAKRVKEG
                     FTQGADIEPIIYDYINSNHAELGLKSPLTGKQQQEITDKFTKHYNTIKESPHFDEFFV
                     ADPDKKGNIFSHQGRISCHFLDFFTRQTKGKHPLGDLASHQEALQEGTSNRLHHKNEV
                     VAQGYEKLDQFKKEVVKLLAENKPKELLDYLVATSPTGVPNYSMLSKETQNYIAYNRN
                     WPAIQKELEKATSIPESQKQDLSRLLSRDNLQHDNLSAITWSKYSSKPLLDVELNKIA
                     EGLELTAKIYNEKRGREWWFKGSRNEARKTQCEELQRVSKEINTLLQSESLTKSQVLE
                     KVLNSIETLDKIDRDISAESNWFQSTLQKEVRLFRDQLKDICQLDKYAFKSTKLDEII
                     SLEMEEQFQKIQDPAVQQIVRDLPSHCHNDEAIEFFKTLNPEEAAKVASYLSLEYREI
                     NKSTDKKTLLEQDIPRLFKEVNTQLLSKLKEEKAIDEQVHEKLSQLADKIAPEHFTRN
                     NIIKWSTNPEKLEESNLNEPIKSVQSPTTKQTSKQFREAMGEITGRNEPPTDTLYTGI
                     IKK"
     gene            complement(11169..13895)
                     /gene="sdcA"
                     /locus_tag="U6309_001656"
     CDS             complement(11169..13895)
                     /gene="sdcA"
                     /locus_tag="U6309_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdcA"
                     /protein_id="HEN5505097.1"
                     /translation="MMNMVDKIKFKEPERCEYLHIAKDNKVHILLPIVGGDEIGLDNT
                     CETTGELLTFFYGKTHGGTKYSAEHHLNEYKKNLEDDIKAIGVQRKISPNAYEDLLKE
                     KKERLEQIEKYIDLIKVLKEKFDEQREIDKLRTEGIPQLPSGVKEVIKSSENAFALRL
                     SPDRPDSFTRFDDPLFSLKRNRSQYEAGGYQRATDGLGARLRSELLPPDKDTPIVFNK
                     KSLKDKIVDSVLVQLDKDFNTKDGDRGQKFEDIKKLVLEEYKKIDSELQVDEDTYHQP
                     LNLDYLENIACTLDDNSTAKDWVYGIIGATTEADYWPKKESESGTEKVSVFYEKQKEI
                     KFESDTNTMSIKVQYLLAEINFYCKTNKLSDANFGEFFDKEPHATEVAKRVKEGLVQG
                     AEIEPIIYNYINSHHAELGLTSELSSKQQEEITEKFTQRYHIIENSPHFDEFFVADPD
                     KKGNIFSHQGRMSCHFLDFFARQTKGKYPLGDLAGHQEALQAGTSNRLHHKNEVVAQG
                     YEKFDQFKKEVVKLLAESKPKELLDYLVATSPTGVPNYSMLSKETQNYIAYNRNWPAI
                     QKELEKTTDIPENQKQDLLRLLSRNNLQHENLSAITWSKYSSKPLLDVELNKIAEGLD
                     LTAKIYNEKRKSEWFKGSRNEARKTQCEELQRVSQEINALLQSESLTKSQVLEKVLNS
                     IEALDKIDRDISAEYNLFKSTLQKEVQSFRDQLKDICQLDNYAFKSTKLDEIISLEME
                     EQFQMIKDPAVQQIVRDLPSHCHNNEVIEFFMTLNPEEAAKVASYLSLEYRELNKSTD
                     KKTLLEQDIPKLFKEVNMELLSQLKQDSAVKEDVYEKFCQLADKIPPEHFTRNNIRKW
                     SANPEKLEESNLGELLKSSEGSITEMARKYRETINEMTGRNESLRETVRNTI"
BASE COUNT         3931 a         2564 c         2820 g         4799 t
ORIGIN      
        1 gcacttatat ttcttattat agaattatat tccaaaaaaa tatattaaat tagaatttaa
       61 aatcaaaaaa aagggtttta ttttgttttt tgcaatccaa tttcaaggga agtaagataa
      121 aatactttat gataattatt taatcctagt ggtcttttta ttttcttttc cgagggtgat
      181 tatgtctgtt tcacgagtta cagcgtggtt tgtgtgggtc atagtatcca tattttacgc
      241 atatcaatat gttctgagag ttatgcccaa tattatgctg gatgatatta ctcagcaatt
      301 tcacattgat gccataattt ttgggcaatt ttccggtatt tattacattg gttattcttt
      361 gatgcacttg cctattggga ttatgctcga tcgttttggc ccaagaaaag tgatgacagg
      421 atgcatcatg ttgaccgtga taggtttact tcccattttg tttgctgaaa attgggttta
      481 tccagtgata ggaagagccc tgattggtat aggttcatcg gctgccattt taggaacatt
      541 taaaataatc cgcatgactt ttaaggagaa acattttcca aggatgctga gcttttcagt
      601 catgatagga cttgttggcg cggtatatgg cggcggtcct gtcagttaca tgtgtcaact
      661 tatgggttat aaaatggtgg tggaaatatt tgtaggcata ggaatagtgc tggctggtat
      721 tacctatcta attgttcccg atgcacagcc ttctcgccag gatagtgtga tttctaatat
      781 cagaatggtt tttggtaata gtaaagtgat cctattgtgt tgttttgcgg gattgatgtt
      841 gggaccactg gaaggttttt ctgatgtatg gggctctggc tttttaaaaa tggcatacag
      901 tttgaatgcc aacgcggcaa attatcttcc atcaatgatt tatattggca tgtgttttgg
      961 cgcacctatc ttaagtctga ttgcagaaaa aacagggtat tatcttggaa ctatcatcgc
     1021 atcaggcgtg gttatgtttc tggtatttgc tatgcttgtc ggaggtgttc tgactattcc
     1081 cagtataaca ataagtttta tcctggttgg catttgttgc tcttatcaaa ttttggctat
     1141 ttataaagta tccacttatg tccccgataa tcttgcaggg ttaactactg caattgcgaa
     1201 tatgattatt atgatttttg ggtatggttt tcattctgta attggttatg tcgtcaatat
     1261 gtatgggggg acacatgtag ctcgcgcttt tgtttatgga gttggggtga ttcctttgac
     1321 attggctgtt ggcgtggtgg gttttttgtt tctggcctat caagagaaaa attttacccc
     1381 ggatgcttcc tttgcctctc ctgaattacg ataattactg gcatttctat cttgtgtggt
     1441 aataaatatc aagggaggta tagctatcat gtatatgttg tattttcatg ttccagaaac
     1501 tcatcttgac gaagtaaaaa atgcagtttt tgcggcaggg gctggttatg ttgataatta
     1561 taaccattgc gcctggcaaa ctttaggtga aggccagttt atgcctttac caggtagcca
     1621 ggcgtttatt ggagaaatta atcaattgga aaaagtgcct gaatacaagg tggaaacgat
     1681 ttgcgccaga gagagaatca aagaggtggt tgcagcttta aaaaaagctc atccctatga
     1741 aagcccttct tatcaagtgg tacgtattga gacagagttc taaatattag agcgagaaat
     1801 tcaggcaggt cgaatcacca gatggttggt catttagtaa gaatttgttt cttcaggata
     1861 cttgttcaat tgagtattca tagataatat ctaatgattg atgaaccaat agttcaaatc
     1921 catcttcaat ctacataagc tgcttttgaa cagataggga aatactggta tacatgttga
     1981 aaatccttgc tctcttaagt tgtttgattc taagctcctg tctggtcggg ccaaatttta
     2041 aagagcctaa aaaagatgcg gccaattatt ggcttacttc aagccctgca ataaaacaat
     2101 ccgcttttca aaatgcgaat tggtggaaag tctttaatga tcccacttta acctcattaa
     2161 tcgataacgg ttatcacaac aatctttcct tgcaaatggc aggagtacga gttttgcaag
     2221 caagagctca acttgctcaa tcagttgggg agctttatcc ccagcaacaa gctatggtag
     2281 gagattataa ttacaatcga attgggggca atcaattaca agggcttcta ccgcccgatt
     2341 ttttaacagc cagcctcgga ttttccgcaa gctgggaact tgatttttgg ggaaaatacc
     2401 gaagagcgat tcaatctaat gatgccctgt tccttgcctc tctggcagct tatgattccg
     2461 cattggttac tctcacctca gatattgcca gtacttttat taaaatccgc accagcgaag
     2521 aattaatcaa agtaacaaaa actaatatcc aggtgcaggc gatgagtctt aaaatcgcaa
     2581 aggatcgtta ttatggtggt caaaccagtt tactggatgt ggaacaagcg gcaacagaac
     2641 ttgcggaaac tcaatcaaaa ctgccccaat acgtgagtga cctccagcac caaaaagata
     2701 ttttggctgt attacttggt accactcctg ataaggtgga tgacttgctt aaaaaaaata
     2761 aaggaatacc aaaagcttta gcggtagtgg aagttggaat tcctaaagaa gtgatagcgc
     2821 gaaggccaga tgtttatcaa gccagaatgg aggctatagc ccaatcagct gcaattggcg
     2881 ctgtgaaggc taatctttat ccttcacttt cattagtggg gacttttgtt tttgcctcaa
     2941 ataatattgg caattctact attagtgata tatttaactg gtctaatcgc aatatagtcg
     3001 caggcccgtc actggcatgg tctttattaa attatggtca aatcacgaac gcggtacgag
     3061 ctcaagacgc tgctttccag caagcattgc tgaactatct taatttggtg ctcaaagtgc
     3121 agcaggaagt tcaagataac attacgcgct atgtagaggg gcaaaaaacc gtctcttcac
     3181 ttaaaacagc caatgctgct gccatagaat cgacaagact tgctttagta cgatacaagg
     3241 aaggagaggc taattacacc actgtattga atgcagaaca gcaacaatta cgagtacaaa
     3301 cttcactggt taatgccaca ggagaattgg ctttgtctct ggttggctta taccgctcgc
     3361 ttggaggcgg ttggcaaata cgcggcaaga atgatattgt ttcaaatcaa ataaaaagtg
     3421 aaatggctgc ccgtactaat tggggtagtt tattgaagca agaaaatcat ttgcctccag
     3481 actctaacgg acaacgaatg aaacaactat atttaccaac ctggtgatga gaatgaactc
     3541 tcaaaataaa caaaatttag ttaaaaccgt agcaattgta ttttttggta ttgtgtttgt
     3601 ttatttagta tatgaccatt ttacaagggc ccaggtagca accccagcac cgccagatgt
     3661 gatcgttcaa aagcccaaat taatgaaaat gggacagtat gttacccaga caggtaatac
     3721 agtagcattt caatcggtag atttggtggc acggattgaa ggttatttgg atgccataaa
     3781 atttactgac ggcgcttttg tcaaaaaaaa tcaggaatta tttattattg aacccgagcc
     3841 ctatcttgag caactgcgtg ctgctgaagc caaggtagcc gctcaaaaag caaattatgc
     3901 ctatgccaaa tcagagtatg ccagacagca acgcatgtat aaacaaaatg ccacgtctct
     3961 taacagtgtt gaaaaatgga aagcaaaagt tgaagaagca caggcagaag tagataaatc
     4021 ggaagctgat gctacaaacg ccgcaattac atatggttat acccacgttt cagctccttt
     4081 cgatgggcgg atgggaagac atttagtcga tgtgggtaat ttggttgggc acggtgccgc
     4141 aacggatttg gctaccatag aacaaatcga ccctatttac gtttatttta atttaaatga
     4201 actggattta atcaggatta gaaatgcagc gcgcaagaga ggatataaac caatggaatt
     4261 ggtcaaaatc cctgttgaag tggctttgca agatgaaaaa ggatttcctc ataaggggac
     4321 attggatttt gtaaatactg gtttaaacgc ttcaactgga accatggagt ttcgcgcctt
     4381 gttacctaac aagggatatc ccctggtacc tggtttattt gtgcaagtcc gggtaccgat
     4441 atctgaacct tctccaaaat taacaatacc tgacacctct gttcaatacg atcaaattgg
     4501 cccctatgtc cttgtggttg ataaagacaa ttatgtgctt accaaacgcg tgacacttgg
     4561 cgggctagaa aatggggtaa gggctattac taaaggcctt gatgctcaag acagtgtgat
     4621 tgtttccggg ttgcaaaatg cgacgcccaa tcatcgggtt acgcctaaaa ctgagcaacc
     4681 tgagtaagta tggccaaaaa attgtaagaa tttaagaccc tttattcagg gagcgaaaca
     4741 actgaaatga tttccaaatt ttttattgaa agacccgttc tggccaatgt cattgccttg
     4801 tttattgtat ttatagggat aatagcgatt gctgttttgc ctgtgtctca ataccctgcc
     4861 attgttcctc ctactatcca ggttactacg acctatcctg gcgctgatgc aaaaactttg
     4921 attaataccg tcgctttgcc aattgagcaa caagtcaatg gggttgagga catgctgtac
     4981 atgcaatcga caagtactag tggaggcacc tatactttaa ttgtcacgtt tgctataggc
     5041 acggatctga actatgcgca ggttctggtg caaaatcgag tacaagcggc gatggctcaa
     5101 ctccccgaat cagtacaaaa gcaaggtgtt atcgttcaac aaaaatcgac tgctattctt
     5161 caatttatta ctttgacttc aaaaaataat gaatacgacg gcgtatttct tgacagctat
     5221 gcgaccatca atatgcaaga tgaattatcc cgtttacccg gagtagggaa tgtggtggtt
     5281 tttggttcag gaagttatgc gatgcgtgtt tggctcgatc ctaaaaagat gcttgctttt
     5341 tctttgaacc caagtgatgt tttaaatgcc atcagctacc aaaacaaaga ggtatcagct
     5401 ggacaactgg gtgctcctcc tactgttggc aaacaagcct atcaatttac agtgaatgtg
     5461 cctggacaac tctctgatcc caaagagttt gaaaacatta ttattaaaac gattgatacc
     5521 ccacccgatc aaaattcctc tgccagtagc tctgctcagg tagtacgtat tcgtgatgta
     5581 gggcgagttg agttgggatc cgagagctat aatcagctcg ctaatcttaa tggtaaacct
     5641 acagcagcaa taggtatctt tcaacttcct ggtgccaatg cgttggatgt tgcgcaagaa
     5701 gttcggaaag ccgtggcgaa aatggcaaaa cagtttcctc cgggtcttga atattctatt
     5761 ccatttgata ccaccgtttt cgtcaaagct tcaattgaag aagtatataa aacacttttt
     5821 gaagcaggaa ttttggtttt aatagtgatt gttgtatttt tacaaaattt tcgcgcttca
     5881 ttagtacccg cgacaacggt tcctgtgact atcattggga cattttttgc catgctttta
     5941 ttgggctata gcattaattt gctgacttta tttgctttgg tattagcaat tggaattgtg
     6001 gttgatgatg ccattgttat tgttgagggt gtgactcaac atattgaaaa aggcttgtca
     6061 cctaaagaat cagccatact tgcaatgaaa gaattatttg gcccgattat tggtatcact
     6121 ttggtattaa tggctgtttt tgtaccggcc ggttttatgc ctgggttaac ggggtcaatg
     6181 tatgcacagt ttgctttagt tattgctgca actgccttta taagcgcaat taatgccatg
     6241 acgttaaagc cgactcaatg tgctttatgg ttaaaggctc ccgatacgag taaaccaaag
     6301 aatatattct ttcaggcctt tgatcgaatt tataatccca ttgaatcggc ttacgtaaag
     6361 tttattgatc ggcttgtaca tcgtagtggt aaagtttgtt tgattggaat cattttagtg
     6421 gcttgtgcca tttatggcct gacgcgtatt ccaactggct ttattcccat ggaagatcaa
     6481 ggctatttga tgttaagtat tcagctgccg gatggcgcca gtttaggacg tactgatgaa
     6541 gtagtgagta gattagctaa aaaagcatct gaagttgggg gtgtagataa tgtgattgct
     6601 attgatggta tttcattatt ggataacaat tctctgcttt ctaatgccgg tgtcatctat
     6661 gttatattca aagattggag tgtcagagga aaatctgaaa atttacgagc tctttatacc
     6721 aaattcaatg caatagccaa agaaaccctt gatgcaaaag tgttggtggt tgtgccccct
     6781 cccattcaag gtttagggat gtcgggggga tttcaaatgc aagttgaatt acaagacggt
     6841 acttttgatt acaggaaatt acagcaagct actgatcaaa tgatcaatac agggcgtcaa
     6901 tacccacagt tgcaaaacct tatgacgaca tttcgtgcga gtgttcctca agttgcagca
     6961 cctattaaca gaaccaaagc cgaatcttta ggagtgcgcg ttgctgatgc ctttgatact
     7021 ttacaaactt atttaggttc ttcttatgtc aatctgttca cgaaatttgg tcaagtattt
     7081 ccagtctatg ttcaggcgga tgcatcctcg agggttagca gcgaagattt aaggaattac
     7141 tatgtaagaa atcaatccgg ctccatggtg cccttaggta ctcttacaga cgtggggcct
     7201 gcggtaggac cctcaattat ttctttatat aacctttatc catcaagtaa cataaatggg
     7261 gttgccgcaa gaggctatag ctcggggcag ggaatccagg ttatggagaa attggcaaag
     7321 gaacaattac ccccagggat atcttatgaa tggacaagta cagcttacca ggaaaaggtg
     7381 gctggtaacc tcagttactt tatttttgcc ttgtctttgg ttttggttta tttaatttta
     7441 tctggccagt atgaaaattg gcttattcca tctgcgatta tcctgagtgt tcctttgact
     7501 ttggttggaa cagtattagc tctcggcagt ttgggtatgg ataataatat gtacactcaa
     7561 attggcttgt tacttcttat tgcattggct acaaaaaatg ctattttgat tgttgaggta
     7621 gcgcgtgaac aacgtgaaat tcataataaa tcagttcttg aggcagcggt cattggcgcc
     7681 aaaacacgtt ttcgtcctat tctgatgaca tcttttgcat ttatcatggg ggttatgccc
     7741 ttggtttttg caacgggagc aggtgccaat tctcgtcgct caattggtat agcggtgagc
     7801 agtggtatgc ttgcctcaac ttgccttgca gttgtttttg tgcctgtttt ttatgtattg
     7861 ttgcaaacct ggcaggacaa gaggaaggca aaacattaat taatttaacg taagttatgg
     7921 ataaagatta cataagaacc agttgatatc cagaggacgt ttgggctgag gagatgcgta
     7981 agcattgtct tgaagccttg gcatgaaaca ttaaatcaaa tacgagattt cgagacggca
     8041 taaatacctc cgcaacctga gtggttcgag ataataactg attttatcca tgactcatgc
     8101 taatttacag ctatagagaa tgctgtgaga ataattctaa tcctctcaca gccaaatagt
     8161 gtatttatct tcctgaacat tcaaacagtt gatgctatca agttatattg tatgcccaag
     8221 ggtgtctgta ggaggctcat ttcttccagt gatttcactc ttacctattt ctttataatt
     8281 cccgtgtaca aagtgtctgt aggaggctca tttcttccag tgatttcacc catcgcttcc
     8341 ctgaattgtt ttgatgtttg tttagtagta gggctttgga ctgatttgat tggctcatta
     8401 agatttgatt cctcaagctt ttcagggttg gtagaccatt ttataatgtt atttcttgta
     8461 aaatgctcag gggcaatttt gtcagccagt tgactgagtt tttcatgaac ttgctcatca
     8521 atagcttttt cttctttgag tttggagagt aactgcgtat tgacttcttt aaacagtctg
     8581 ggaatatctt gttctaggag agttttctta tcggttgatt tattaatttc cctgtattcc
     8641 aggcttaaat agctagctac ttttgctgcc tcttcagggt tcaatgtctt aaagaattca
     8701 attgcttcat cattgtggca atgagaaggc aagtccctga caatttgttg aacagcagga
     8761 tcttgtatct tttgaaattg ttcttccatt tccagagaga tgatttcatc aagttttgtt
     8821 gatttaaagg catacttgtc caattggcaa atatctttca attgatctcg aaataacctg
     8881 acttcctttt gcagagtact ttgaaaccaa ttggattcgg cagaaatgtc tctgtcaatt
     8941 ttatctaatg tttctataga atttaaaacc ttttcaagta cctggctttt cgttaaagat
     9001 tcactttgca gaagagtatt gatttctttg gatactcttt gcaattcttc acattgggtc
     9061 ttacgagctt catttcttga acctttaaac caccattcgc gtcctctctt ttcattgtaa
     9121 atttttgcag tgagttctaa tccttcagcg attttattta attccacatc caataatggc
     9181 ttggaggaat attttgacca ggtaattgcg cttagattat cgtgttgtaa attatcacga
     9241 gaaagcaatc ttgaaagatc ttgtttttga ctctccggga tgctggtagc cttttccagc
     9301 tctttttgaa tggctggcca gttacgatta taagcaatgt aattttgagt ttccttcgaa
     9361 agcatggagt aatttggaac acctgtaggt gaggtagcaa ccaaataatc caataattct
     9421 tttggtttat tctcagccag cagtttgaca acctctttct tgaattgatc cagtttttcg
     9481 tacccctggg ctactacctc attcttgtga tgtaagcgat tggaggttcc ttcctggaga
     9541 gcttcctgat gacttgcaag atcaccaaga ggatgtttgc ctttggtttg tcgagtaaag
     9601 aaatccagaa aatgacaact gattctgcct tgatgagaaa agatattgcc ttttttatcc
     9661 ggatcagcga caaaaaactc atcaaaatgt ggagattctt taatcgtatt ataatgtttt
     9721 gtaaatttat cagtgatttc ttgttgttgt ttgccggtta acggagattt taatcccagc
     9781 tcggcatggt tgctgttaat atagtcgtat ataattggtt ctatatctgc accttgcgta
     9841 aatccttcct ttactctttt cgcaatttca gtagcatgag gctctttgtc gaaaaattct
     9901 ccaaagttag catccgataa tttgtttgtt ttacagtaaa tattggcttc ggccaacagg
     9961 tactgaactc tgatggatat cttgtccgca tcgcggtctg atgatatttc tttagcacca
    10021 tcatagaaag ggctggatgc ctgagctacc cagacagtgg aatctactgt agcgtcgaga
    10081 atagtatcaa tccaatcacc aatttcagag tcgttatcta caagcatcaa actatttact
    10141 aaataatcgt aatttatttc ttttcctctt ggatctttag ataaagatag tttcgaatat
    10201 tgaggatcac tttttactaa ctcttttaca agttcgtttt gaagattagt aattagttcg
    10261 tttaattttt catctttttt ttctccatca attctatctt catctttata atgactaaga
    10321 acagtgcttt taactttatc cctaagtgat tttttattaa ttggagttgg tgttttatct
    10381 tccgggaaca gtgttgaacg caggcgataa cctaatccct cgtatattgg aataggagct
    10441 tgtcttgagg gcgtgtcata ttttgagatg tttcttttga cattgaataa agggtcatca
    10501 aagcgagtga atttatcgtt gtcatatgga gaaagtctca cagcgaaagc attttcagag
    10561 gatttaatga tttcctttac cccagagggt aattgcggaa tccctccagt acgaagttgc
    10621 ctgatatcat tttgttcatc atattgtttt tttagtactt gaattaattc aatgtatttt
    10681 tcaatttgct gtaagcgttc tattttctct tttaataaat cgtcataagc atgaggtgaa
    10741 attttctttt gactattgat ggctttgatg tcttcttcca attgcctttt gtattcactg
    10801 agttggtgtt cagcagaata tttagtcaca ccactgtgcg cactaccata gaaaaatgtg
    10861 atcaactcaa ctgctgtttg acaggtatta tccaggccta tttcatctcc tcctacaatc
    10921 ggtaaaagga tatgaacttt gttgttttca tcaacatata gataatcaca acgttccggc
    10981 tctttgaatt tgattacgtc aaccatgttt atcaccattt tctagtgtct tgtttataag
    11041 tatattataa agacaatttg tttgtaaaac aacaaaaaga gttgtaatta aactttttgc
    11101 ttgattacac atacgcctca acacaaccgt gtttttatat tctcaatcgc ttgattcata
    11161 ctactaagct atattgtatt cctaacagtt tctctgagtg actcatttct tcctgtcatt
    11221 tcatttatgg tttctctgta ttttcttgcc atttcagtaa ttgagccttc agaggatttt
    11281 agtaattcac cgagattgga ctcctcaagt ttttcaggat tggcagacca tttcctgata
    11341 ttattccttg taaaatgctc aggaggaatt ttatcagcta attgacagaa tttttcatag
    11401 acgtcttcct taactgcact atcttgtttt aattgggata atagttccat attcacttct
    11461 ttaaataatt taggaatatc ttgttcaagg agagttttct tatctgttga tttattgagt
    11521 tcccggtact cgaggcttaa atagctagct actttagctg cttcttcagg atttaatgtc
    11581 ataaagaatt caatcacttc attattatga caatgagaag gcaagtctcg gacaatttgt
    11641 tggacagcag gatctttaat catttgaaat tgttcttcca tttccagaga gataatttca
    11701 tcaagttttg ttgatttaaa ggcatagtta tctaattgac agatatcctt caattgatct
    11761 cgaaatgatt gtacttcttt ttgcagagta ctcttaaata aattatattc agcagaaatg
    11821 tctctatcaa ttttatccaa tgcttctatg gagtttaaaa ctttttcaag aacttggctt
    11881 tttgttaaag attcactttg cagaagagca ttgatttctt gggatactct ttgcaattct
    11941 tcacattggg tcttacgagc ctcatttctt gaacctttaa accattcact ttttcttttt
    12001 tcattgtaaa ttttagcagt tagatctaaa ccttcggcga ttttatttaa ctccacatcc
    12061 aataatggct tggaagagta ttttgaccag gtaattgcac tgaggttctc gtgttgcaga
    12121 ttattacgag aaagcaatct taaaagatct tgtttttggt tctctggtat gtcagtagtt
    12181 ttttcgagct ctttttgaat ggctggccag ttacggttgt aagcaatata attttgagtt
    12241 tcctttgaga gcatggaata attaggaaca cctgtggggg aggtagcaac caaataatcc
    12301 aataattctt ttggtttact ctctgccagc agtttgacga cttctttctt gaattgatca
    12361 aatttttcat atccttgagc gactacctca ttcttgtgat gtaaccgatt ggaagttcct
    12421 gcctggagtg cttcttgatg acctgcaaga tcaccaaggg gatatttgcc tttggtttgt
    12481 cgagcgaaga aatccaggaa atggcaactc attctgcctt gatgggagaa tatattccct
    12541 tttttatcag gatcagcgac aaaaaattca tcaaagtgag gagagttctc aataatgtga
    12601 taacgttgag taaatttttc agtaatttcc tcttgttgct tgctgcttaa ctcagatgtt
    12661 aatcccagtt cggcatggtg gctgttaatg taattgtaaa taataggctc aatctctgct
    12721 ccttgaacaa gtccttcttt cactctttta gcaacttcag tagcatgggg ctctttatca
    12781 aaaaattcac cgaagttagc atctgataac ttgttggttt tacaataaaa attaatctca
    12841 gctaatagat attggacttt aattgacatt gtatttgtat cagattcaaa ttttatttcc
    12901 ttttgcttct catagaatac actaactttt tcagtaccac tttcactttc cttttttggc
    12961 caataatcag cttctgttgt agcaccaata attccataaa cccaatcttt cgcggtggag
    13021 ttgtcatcta acgtacatgc tatgttttcc aaataatcca agttaagtgg ttggtgatag
    13081 gtatcctcat ccacttgaag ttcggaatca attttcttgt actcttctag aacgagtttt
    13141 tttatatcct caaatttttg accacgatcg ccatcttttg tattaaaatc tttgtcaagt
    13201 tgcactaaaa cagaatctac gattttgtcc ttcagtgatt ttttattaaa aacaatagga
    13261 gtgtctttat ctggtggtag aagctcagaa cgcaaacgag cccctaatcc atcagttgcc
    13321 cgttggtatc ccccagcttc atattgagat ctgtttcttt tgagactaaa cagaggatca
    13381 tcaaagcggg taaatgagtc tggtctatcc ggggaaagcc ttaaagcaaa ggcattttca
    13441 gaagatttaa tgacttcctt tactccagaa ggtaattgcg ggattccttc tgtgcgtagt
    13501 ttgtctattt ctctttgctc atcaaatttc tctttgagta ctttgattaa atcaatatat
    13561 ttttcgattt gttccaggcg ctcttttttc tcttttaata gatcttcata ggcatttggt
    13621 gaaatttttc tttgaacacc gattgccttt atatcatctt ccagattctt tttatattca
    13681 tttaggtgat gttcagcaga atactttgtc ccaccatgcg tttttccata gaaaaaggtt
    13741 aataactcac ctgttgtttc acaggtatta tccagtccta tttcatcccc acctacaata
    13801 ggtaaaagaa tatggacttt attatcctta gcgatatgta aatattcgca tcgctccggc
    13861 tctttgaatt ttattttgtc aaccatgttc atcaccctat gctagtgttc tgactataag
    13921 tatattataa aatgattata agtatttaaa aataagtctg attgttaaaa agatgataat
    13981 atttgtgttt tttctttcag tataaaaaac taatcattgt tcctcatata caagaattga
    14041 gctcacaatt tgagttatct aaaacaatcg aatatgagat tttcgattaa atcattgcga
    14101 ttttttgagt tatg
//
LOCUS       DASLOE010000072        13950 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.072, whole genome shotgun sequence.
ACCESSION   DASLOE010000072 DASLOE010000000
VERSION     DASLOE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13950
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.072"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1185
                     /locus_tag="U6309_001657"
     CDS             <1..1185
                     /locus_tag="U6309_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN5505098.1"
                     /translation="AIQFVNKFIIELESKDKDSSTVNYLKQHGFYRILYRHPKLLGQE
                     LPQDDILHKLLRSGFIGVGISAVMILLFISTVFFTAPLWLTAITTGLFVGASTYLSGI
                     LYGVVNDIFATHANLPYFLLGHQSQQTSLLRTNDKVAQGIAWGVAATFGPVIIATLIF
                     TVAATITAFFVPIATFLLPIMMIAMPLIAVGAEFYARKKTREYLDTEGNFYWIGSNDY
                     QRRGLNYMCPTNEERAAWYANSDRNLFGFTKVPLIGLGALIGLVVLSGISMFLPPVLF
                     ISPVIAIAIPAAFSAVTCATLIAGGIYMQVNRNKQLDDRYRLEFERDEIVPDLYLDED
                     MEYIRKIAKIYAQNEPTVEPKQEVINTENYSFLFDKKPKQAMPNIKQEEFEPARLSQA
                     GA"
     gene            1445..2311
                     /locus_tag="U6309_001658"
     CDS             1445..2311
                     /locus_tag="U6309_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0189 family type II secretion system
                     effector"
                     /protein_id="HEN5505099.1"
                     /translation="MRFNHFIAPLVLLPVCALSQPQNDKGDVFLSKIPISKNAFVKSY
                     SSESAEFKLLESNTRSIDYPTQIIRISGSLESQQLDCDQVHAEILEKVVAPFAPDKFT
                     YNTYISCSYNPDTNMATGFLINSYIDPLNDDAVELLETYLAKNNGSNLLGTPLTFEPA
                     KALIVALTISAGMKKNPNKPPFVEYKQDRSNFYFKSNYEMRNKLFTDIYQNFFTNDPN
                     KILPFLDKWIFVNASSVYEAILMDSNFVELQPERIFLMENGEQIFVSNLKYYFTHNCN
                     RYSNHRCLQPQA"
     gene            complement(2477..3535)
                     /locus_tag="U6309_001659"
     CDS             complement(2477..3535)
                     /locus_tag="U6309_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEN5505100.1"
                     /translation="MTEEVLFSQEGQLGFITLNRPKALNALTLTMIMALQKQLSIWKE
                     DNSIKAVVVQAVPGNAFCAGGDIRWLYNAGRSKDSEQMQFFWHEYRLNHFIHHFGKPY
                     ISLLDGITMGGGVGISLHGSHPVASERFVFAMPETGIGFFPDIGASYLLNKCPGFLGV
                     YLGLTGNKLGPHDARKAGLVKQIVFAEQMQSMIEALKREDLSEDAFNRVDQCISSFAS
                     DAMPTEASQIKPLIDVCFSKPSVELIRESLQSTDGVWALGVDNTLLQKSPLSLKITLA
                     QIQKAKGLSLAECLKMDFDLASHFMKGSDFYEGVRSLLINKDKNPQWRPSSLELVTDA
                     MVVSYFESSSSGLELMIL"
     gene            complement(3538..4314)
                     /locus_tag="U6309_001660"
     CDS             complement(3538..4314)
                     /locus_tag="U6309_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEN5505101.1"
                     /translation="MNLIEQDIDNNGILTLTLNRPEKLNALSTDVLSALNELFFQAKS
                     NPKVKALLLTGNGKAFCAGADINRLAECNAQTGYEFACQGQEVFRLLETMGKPSLAAV
                     NGFAFGGGCELAISATLRIASNKAMFGQPEVKLGVIPGYGGTQRLARLIGKGRAMDLC
                     LTGRFINAETALNWGLVSEVVAPEDLLTQGKNILNGILSMAPLAVASVMEVIDHGYDL
                     SLTEALHLEAIHFAKVCATEDKKEGVAAFLDKRTADFKGV"
     gene            complement(4331..5485)
                     /locus_tag="U6309_001661"
     CDS             complement(4331..5485)
                     /locus_tag="U6309_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEN5505102.1"
                     /translation="MHFNFTDEHLAFREMASEFARNKLAPMADFWDEQGYFPIEILRE
                     AAQLGMAGMVVREDIGGAQLSRLDAALIFEQLATGCIPTSAYLSIHNMVASLVDRYGS
                     QEIRKKWGPKLTSMEVIASYCLTEPESGSDAASLKTRAVKEGEYYVLNGAKAFISGGS
                     VSDVYLCMVRTGDESHHGISCLLIEKNTPGLTFGKLEKKLGWRNQPTCMLYFENCRVP
                     IANRVGEEGMGFKIALNALNGGRVNIASCSLGGALACLRMSQSYMHERKQFGKPLTQM
                     QALRFYFADMLTDYEAARLMVYRAAAAMDNDDPNAPMYCAMGKRLATDVAFRISDKAM
                     QLHGGYGYLRDYQIERIFRDLRVHQILEGTNEIMREIIAKASLDEEYFIE"
     gene            5599..7425
                     /gene="recQ"
                     /locus_tag="U6309_001662"
     CDS             5599..7425
                     /gene="recQ"
                     /locus_tag="U6309_001662"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEN5505103.1"
                     /translation="MGTLAQPSFINPNALAVLKKYFGFDSFRPPQEKIIEDVISGEDV
                     LVLMPTGGGKSLCYQIPAIVRRGVGIVVSPLIALMEDQVTALKLQGIRAAYYNSSLTA
                     EEARNVLNQLHHNELDLLYIAPERLISTSFLDRLSECTISLFAIDEAHCISQWGHDFR
                     PEYACLGLLKTNFPDIPMIALTATADKQTREDIVTKLNYQPKKYVVSFNRPNIHYKVV
                     PKTNAVKQLNLFLQSDTQQSGIIYCGTRHTVEHLTAKLQELGLKARAYHAGLSHTERR
                     EVQNLFRYDRIDIVVATIAFGMGIDKPNVRFVVHHDLPKSIEGYYQETGRAGRDGLPA
                     KALLLYDAADSARLRSWIMNIPLDEQRHIETNKLNHMLAFAEASHCRRQILLRYFDEP
                     SHTECKNCDVCDNPPQTADATEDAQKFLSCIYRLKQSYGLTHTIDVLRGSLSEKIKQS
                     GHEHLSTFGIGKEKSANYWKHLAWQLIHKDYCLQDMNHFNVLRLTPKAIPLLKGEESI
                     SLTIPNNDLNSSKKKGKQSPSFKPESNPLFEKLRALRRQLADEENKPPFMIFSDATLH
                     EMAQLKPKNSKQLLNVSGVGQHKLARYGHYFLEALNEFSEVE"
     gene            7435..7986
                     /locus_tag="U6309_001663"
     CDS             7435..7986
                     /locus_tag="U6309_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEN5505104.1"
                     /translation="MRKLPRPFYERDTILVAKELLGKYLVHHDGLEEKIGRIVEVEAY
                     LGQHDLACHSSKGLTKRTKVMFGPAGYAYVYLIYGMYYCMNVVTEKEGIGSAVLIRAL
                     EPIKNIQDRTQGPGLLSKAMRIDSKLNHRDLLSNDFYIAEPNSPTDFTIIEKPRVGVH
                     YAKEWANELLRFYIKDNPYISKT"
     gene            complement(8181..8585)
                     /locus_tag="U6309_001664"
     CDS             complement(8181..8585)
                     /locus_tag="U6309_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HEN5505105.1"
                     /translation="MKKLLSASVITLMITGCAAIPADPQAERVIASPNPAPKGCKYVG
                     QVVGNQGNFFTGGFTSNRNLEEGAMNDLKNKASKKGANYIQLVTNRAGVTGSMSGSFD
                     RHGGFMSGGSEQTNVTNLGNAYICPPKAIGLE"
     gene            complement(8641..8844)
                     /locus_tag="U6309_001665"
     CDS             complement(8641..8844)
                     /locus_tag="U6309_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HEN5505106.1"
                     /translation="MTDKVYQIVELIGTSQEGIEKAIDNAIKQVAKTHGKLDWFEVVE
                     TRGFIDDNKVKYYQVHLKVGCNV"
     gene            complement(8834..9520)
                     /locus_tag="U6309_001666"
     CDS             complement(8834..9520)
                     /locus_tag="U6309_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN5505107.1"
                     /translation="MNEWWLIILLVVLAVFACTLIIYPLRRNRLVSLLLAPIIFIMVF
                     TGYYFWGDFARWQEYLHRNEAQELAQSMLKSIKSPQELIDKLKAKLDAQPESAKGWYL
                     LGRLYSSQKDNQNASLAFAKAHQLKPEDEQFAVNYAHSLWQLSNQQFNPDILEIFHTL
                     LKNNPRQPDALAMLAMHAYLSHDYEAAINYWQRLLQLAPAQSEEALAIRKAIAKAEKQ
                     INRGGKKSDR"
     gene            complement(9513..9914)
                     /locus_tag="U6309_001667"
     CDS             complement(9513..9914)
                     /locus_tag="U6309_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEN5505108.1"
                     /translation="MRVFHFCFKVILLLLLVSVAFASSMYPLDSARKEAQFNHLLKDL
                     RCLVCQNQDLADSNAQLAKDLRFQVYELVKEGKSDSEIMDYLTARYGDFILFKPPVKS
                     VTLLLWFGPFLFLLIGFIIFWRTCFKRNSHE"
     gene            complement(9914..10447)
                     /locus_tag="U6309_001668"
     CDS             complement(9914..10447)
                     /locus_tag="U6309_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEN5505109.1"
                     /translation="MKKIGWRVIPIAIFCLLCFFLWRGLSLDPHHLPSARIGKPLPDF
                     ALPELQNPDSTFSAMNFHNQVVLLNVWASWCDACVEEQVFMLQLAREGVPIYGLNYKD
                     NPEHARAWLAQWGNPYRLIGQDSEGKVAIDLGVYGAPETFVIDKKGIVRYRHVGIMNQ
                     KTWLTDILPLMRNLEKS"
     gene            complement(10444..12396)
                     /locus_tag="U6309_001669"
     CDS             complement(10444..12396)
                     /locus_tag="U6309_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEN5505110.1"
                     /translation="MIAELGLFFLILALVASLMLALIPLAGLQFNRKDWIDSSRNYVV
                     LQFLFVACAYLCLTACFLMDDFSVIYVLNNSSLLLPWFYKFCAVWGGHEGSMLLWVAI
                     LSFWMILVCFFSSSLDTEMRARVLVILGWLSIGFILFLLMTSNPFLRQFQVLNTQGRD
                     LNPLLQDPGFLFHPPMLYMGYVGFCVAFAFAIAALWAGKVETSWSKWTRPWTLAAWCC
                     LTAGITLGSWWAYRELGWGGWWFWDPVENASFMPWLVGTALLHSLAVTEQRQQFKAWT
                     MLLAIAAFSLSLIGTFLVRSGVLTSVHAFAVDPQRGLYILGFLLFVIGGSLTLFLFRA
                     QTLQVNKHPGLLSRESALLLNNVFLVVIMLTVLMGTVYPLLVEGLGLGKLSVGAPYFN
                     AVFVPLIIPLLLLMGLGVRLKWQNDCWRDVFRKLNRIFLLSLILPVILLFCTSTEIHL
                     TALLGLILAFWVLLSTANAAYHKVRDRGVAGVGQAYWGMVLAHCGVAATVIGIAVSSA
                     YGVQDDVRMSPGKQVSLLDYQIGFINQTPLNGPNYKGTRAEFSIQYQNKTRQIFPEKR
                     VYTIGQMPMTESAIDVTPFRDIYIALGEPLSEDAWSVRIYYKPLVRWIWAGGFMILAG
                     GVLALTDRRYYRKRQVTVMAGQEIKI"
     gene            complement(12393..12824)
                     /gene="ccmE"
                     /locus_tag="U6309_001670"
     CDS             complement(12393..12824)
                     /gene="ccmE"
                     /locus_tag="U6309_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEN5505111.1"
                     /translation="MTPVRRRKLFILLFALSVLSAAAALVLYALRQNISLFYTPTQIV
                     AGEAPAKHHIRVGGMVEANSIVRAKKGLDVQFKITDFENTIVVTYSGILPDLFREGQG
                     IVAEGEVTDNHHFHAIQVLAKHDANYMPPQVKSALADKVKQ"
     gene            complement(12830..12988)
                     /gene="ccmD"
                     /locus_tag="U6309_001671"
     CDS             complement(12830..12988)
                     /gene="ccmD"
                     /locus_tag="U6309_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEN5505112.1"
                     /translation="MNQFFEWLAMGGYSLYVWPAYGLVCVVLGMNILGIKWQRTRTRK
                     KLQRWFKR"
     gene            complement(12972..13727)
                     /gene="ccsA"
                     /locus_tag="U6309_001672"
     CDS             complement(12972..13727)
                     /gene="ccsA"
                     /locus_tag="U6309_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEN5505113.1"
                     /translation="MWKILYQLASPKNFYNYAGRLIPWLAVSALATIAIGMVWGLVFA
                     PPDYQQGDAYRIIFVHVPSAFLSMALYAWMGFLAILLLVWRIKMAGLLIHKVAQLGAC
                     MAFLALITGSIWGKPMWGAWWVWDARLTSELILLLLYLAILATYQAVKNKEDGDKIIA
                     ILALVGLIDLPIIHYSVYWWNTLHQGATLSVFAKPKIAPSMLYPLLITLLGFFLYSLW
                     IILEKARNEVLFRERKQSWVKVQFEEESDESVF"
BASE COUNT         4437 a         3096 c         2427 g         3990 t
ORIGIN      
        1 gccattcagt tcgttaataa atttattatt gaattagaat caaaagataa agacagctct
       61 acagttaatt atttaaaaca acatggtttt tatagaatac tataccgtca tcctaaatta
      121 ttaggtcaag aattgccaca ggacgatata ttacataaat tattgcgctc tggatttatt
      181 ggtgttggta tttcagccgt aatgattcta ttgtttatct ccactgtttt ttttactgcc
      241 cccttatggt taacagctat cacaaccggt ttatttgtag gtgcctctac ctatttaagt
      301 ggaattcttt atggcgtagt aaatgatatt tttgcaacac atgctaactt gccctatttc
      361 ctgttaggcc accagtctca acaaaccagt ctgttaagaa ctaacgataa agtagcgcaa
      421 ggaatagcat ggggcgtcgc tgcaactttt ggtccagtaa ttatcgcaac cctgatattt
      481 acagttgcag caacaattac ggcttttttt gttccaatag ctaccttttt attaccaata
      541 atgatgattg caatgccatt aattgcagtt ggcgcagaat tttatgctcg caaaaaaaca
      601 cgcgaatatc tggataccga gggaaatttt tattggatag ggtctaatga ctatcagaga
      661 agaggtttaa actacatgtg cccgactaat gaagagcgag cagcatggta tgcaaactcc
      721 gatagaaatt tatttggttt taccaaggta cctcttattg gtctgggggc gctgattggg
      781 ttagttgtac taagtggtat tagcatgttt ctaccaccag tgctctttat ctcgccagtt
      841 atagcaatag cgattccagc agccttttct gctgttacct gcgctacttt aatcgctggc
      901 ggtatctata tgcaagtcaa tcgtaataaa caattggatg atcgttaccg tctcgagttt
      961 gaacgtgatg aaattgtacc tgatttatat ctggacgaag acatggaata tattagaaaa
     1021 attgcaaaaa tatatgctca aaatgaacca accgttgagc ccaaacaaga agttatcaac
     1081 actgaaaatt atagtttcct ttttgataaa aaaccaaaac aagcaatgcc aaatataaaa
     1141 caggaagaat ttgaaccggc acgactgagt caggctggag cctaatagtc ctatatgaac
     1201 ggattcaaag acacgtttag tactcaatca attagtagcg ctctattatt gccaaaagag
     1261 cgctgcccga attaattcca agaaactttg catatccatg atgtagccaa tttgcatcat
     1321 tcctgtatac tcaaaattag aacctgtttt catgcgatca cttgtgatca aaacctgatc
     1381 taatctgttc cctgctaaac tggttcttta aattaaagga tttaatttaa aaaaggagtt
     1441 tgctatgaga ttcaatcatt ttattgctcc actggtttta ctccctgttt gtgcattaag
     1501 ccaaccccaa aatgacaaag gagatgtctt tttatcaaaa attccaatca gtaaaaatgc
     1561 ttttgtaaaa tcttattcca gcgaatcggc agaattcaag ttacttgaat ctaataccag
     1621 aagcattgac tatcctactc aaattattcg tatctctggt tctctcgaat cacaacagct
     1681 ggactgcgat caagtacacg cagaaattct tgaaaaagtg gtggctcctt ttgctcctga
     1741 taaatttact tacaatactt atattagttg tagttataat ccggatacca atatggccac
     1801 tggctttctc atcaatagtt atatagaccc actaaatgat gatgccgttg aattattaga
     1861 aacttacctg gcaaaaaata atggttccaa tctgttaggt acaccattga catttgagcc
     1921 cgctaaagcg cttatcgttg ctttaactat ctctgcagga atgaaaaaaa atcctaataa
     1981 accacccttc gtagaatata agcaagaccg cagcaacttc tacttcaaaa gcaattatga
     2041 aatgagaaat aaattgttca ctgatattta tcaaaatttc tttaccaatg atcccaataa
     2101 aattctacct ttcctggata agtggatttt cgttaatgcc tcaagcgttt atgaagccat
     2161 tttaatggac tccaattttg tcgaactgca acctgaacga atttttctta tggaaaatgg
     2221 tgaacaaata tttgtttcca atctaaaata ttatttcact cataattgta ataggtacag
     2281 taatcaccgc tgcttacaac cgcaagcttg aagctctttt gacgaaggag acatttttgg
     2341 gatgagcaca ttttattaaa ttattacttt tagatacttc cttatgacag caagcagata
     2401 atatttcaga ctgctttcaa ccataaggaa gtattcatac gggaaatcta ttattccccc
     2461 aatcaaaatc caaaaactaa agtatcatta attccaatcc ggaagatgag ctttcaaaat
     2521 aactcactac catggcatca gtgaccaact ccaagctgga cggtctccat tgaggatttt
     2581 tatctttatt aatcaataaa gagcgaactc cttcataaaa atcactacct ttcataaaat
     2641 gactggcgag atcgaaatcc atttttaaac actctgctaa agataagccc ttggcttttt
     2701 ggatttgtgc aagggttatc ttaaggctca gaggcgattt ttgcagcaaa gtattatcca
     2761 ccccaagagc ccacacaccg tctgtgctct gtaaagactc tcttattagt tcaacactag
     2821 gcttggagaa acagacatcg attaaaggtt tgatttgact tgcctcagtg ggcatggcat
     2881 cacttgcaaa agaagaaatg cattgatcaa cacgattgaa agcatcttct gataaatctt
     2941 ctctctttaa tgcctcaatc attgactgca tttgctctgc aaagacaatc tgcttcacta
     3001 accctgcctt cctggcatca tgaggaccca atttatttcc ggttaacccc aaatagactc
     3061 ctaaaaaccc tgggcatttg tttaataaat agctggcacc aatatcagga aaaaaaccaa
     3121 tccctgtttc tggcattgca aatacaaaac gctcactggc aacagggtgg cttccatgta
     3181 aggaaatacc gacccctcct cccatagtaa tcccatccag caaagaaata taaggtttac
     3241 caaaatgatg aataaaatgg tttaacctgt actcatgcca aaagaattgc atctgctcag
     3301 agtctttact acggcccgca ttatatagcc aacgaatatc accacctgcg caaaaagcat
     3361 tacccggaac agcttgtacg acgactgctt taatagaatt atcttctttc caaattgaca
     3421 attgcttttg cagtgccatg atcatggtta aagttaaagc atttaaagcc tttggtcgat
     3481 ttaaagtaat gaatcccaat tgaccttcct gagaaaacag cacctcttca gtcataatta
     3541 aaccccttta aaatccgcgg ttctcttgtc tagaaacgca gctactcctt ccttcttatc
     3601 ttcagtggca caaacttttg caaaatgaat tgcttccaaa tgaagtgcct ctgtgagtga
     3661 taaatcataa ccatgatcta tcacttccat tacactggca actgctagag gagccatgct
     3721 taatattccg tttagaatat tttttccttg agtcaacaaa tcctctggtg ctacaacttc
     3781 actgactaac ccccagttta aggcagtctc cgcatttata aagcgcccag tcaaacataa
     3841 atccatcgcg cgccctttgc caattagcct ggccaatcgt tgtgtgccgc catacccggg
     3901 aataacacct aattttacct caggctgacc aaacattgcc ttatttgaag caattctaag
     3961 agtcgcagat atcgctaact cacatccgcc accgaaagca aaaccattca ctgcggcaag
     4021 tgaaggtttc cccatcgttt ccaaaagtcg aaaaacctct tgtccctggc aagcaaattc
     4081 ataaccagtt tgagcgttac attcagccag tcgattaatg tcagccccag cgcaaaatgc
     4141 tttaccattt cctgttaaca ataatgcttt gactttggga ttggatttcg cttgaaagaa
     4201 taattcattt aatgcgctca gaacatcagt gcttaaagca ttgagcttct ctggcctatt
     4261 caaggttaaa gttaagattc cgttattatc tatatcctgt tcaattaaat tcatcaataa
     4321 tcccctatga ttactctata aaatactctt catccaaact tgctttcgct ataatttcac
     4381 gcatgatttc attagtgcct tcaagaattt gatgaactct taaatctcta aatattcttt
     4441 caatttgata atctcgcagg tagccataac ctccatgcaa ttgcatggct ttatcactaa
     4501 tacgaaatgc gacatctgta gccaagcgct tgcccatagc acaatacatt ggcgcattgg
     4561 gatcatcatt atccattgct gctgcagccc tataaaccat cagccttgcc gcttcataat
     4621 ctgttaacat atccgcaaaa taaaaacgaa gagcttgcat ctgagttaaa ggttttccaa
     4681 actgtttgcg ctcatgcata taagattggc tcattcgcag gcaagcaagg gcccctccta
     4741 atgagcagga agcaatattc actctccctc cgtttaaggc attcagtgca attttaaaac
     4801 ccataccttc ttcaccaaca cgattggcaa tgggcacacg acaattttca aaatatagca
     4861 tacaagttgg ctgatttcgc caacctaatt ttttctccag tttgccaaat gtcagaccag
     4921 gagtattctt ttcaattaac aaacaactga tgccatgatg agactcatca cctgtacgta
     4981 ccatacataa atacacatca ctaacacttc cacccgatat gaatgctttc gcaccattaa
     5041 gaacataata ttcaccctct tttaccgcgc gagtttttaa agaggctgca tcagatcctg
     5101 attctggctc tgtaagacaa taactggcta tcacttccat actggttaat tttggtcccc
     5161 atttcttgcg aatctcttgg ctgccatagc ggtcgaccaa tgaagcgacc atattatgga
     5221 tggagagata tgcacttgtt ggaatacagc ccgtagcgag ttgttcaaag atgagtgctg
     5281 catccagacg agagagctga gcacctccta tgtcttcgcg gactaccatt cccgccatac
     5341 ctaattgcgc cgcttctctt aaaatttcga ttggaaagta accttgttca tcccagaaat
     5401 ctgccattgg agccagtttg ttacgagcaa actcggaggc catttctcga aatgccaaat
     5461 gttcatcagt gaaattaaaa tgcatagacc atccttagat ttattgattt tagtcattgg
     5521 atttatttat gacgctttct taaaaaatga tattattgcg aatttttagc gcccaggaaa
     5581 gataataaga ataattacat gggaacttta gcgcaaccat cttttataaa tccaaacgca
     5641 ttagctgttt taaaaaaata ttttggattt gattccttca gacctcctca ggaaaaaata
     5701 attgaggatg tcatttctgg ggaagatgta ctggttttga tgcctactgg agggggaaaa
     5761 tcgctttgct atcaaattcc agccattgtt cgccgtggtg ttggcattgt agtatctcct
     5821 ttaatcgcgt taatggaaga tcaagtaacc gctctcaaac tgcagggtat tcgtgcggct
     5881 tactacaact catccttaac cgccgaagag gctagaaatg tattaaatca attacatcat
     5941 aatgaattgg atttactcta tattgctcca gaacgtctta ttagtacctc gtttctggat
     6001 cgactgtcag aatgtactat ctctttattt gctatcgatg aagcacactg tatttcacaa
     6061 tggggacatg attttcgacc tgaatacgcc tgtttaggtt tactgaaaac taactttcct
     6121 gacattccta tgattgctct gacagctact gccgataagc aaacacgtga ggatattgtt
     6181 accaagttga attatcaacc caaaaaatac gttgtttcat tcaatcgacc gaatatccat
     6241 tacaaagtag ttcctaaaac aaatgcagtg aagcaattaa atctgttttt acaatctgat
     6301 acccagcaat ccggaattat ttattgtgga acccgccata cagtcgaaca tcttacagcg
     6361 aaattacaag aacttggatt gaaagcacgc gcttatcatg caggcctgtc tcatacggag
     6421 cggcgtgagg tgcaaaacct tttcagatac gatcgcattg atattgtggt agctacaatt
     6481 gcttttggaa tgggtattga caagcctaat gttcgctttg ttgttcacca tgatttaccc
     6541 aaaagcatcg aaggttacta ccaggaaaca ggtcgtgcag gacgtgacgg attaccggcc
     6601 aaggcccttc ttctttatga tgctgcggat agtgcccgtt tgcggtcctg gataatgaac
     6661 atccctcttg atgagcaaag acatatagaa accaataaat taaatcatat gcttgctttt
     6721 gctgaagcgt cgcattgtcg tcgtcaaatt ttattacgct attttgatga accttctcat
     6781 acagaatgta aaaactgtga tgtctgcgat aacccaccgc aaacagccga tgcaacagaa
     6841 gatgcgcaaa aatttttatc gtgtatttat cgattaaaac aaagctatgg tttaactcat
     6901 actattgatg ttctgagagg tagtttatct gaaaaaatca agcagtctgg gcatgagcat
     6961 ttaagtacct ttggaattgg gaaagagaaa tctgccaatt attggaagca tcttgcctgg
     7021 caattaattc ataaagatta ttgtttacag gacatgaatc attttaatgt gttacgcttg
     7081 accccaaaag cgattcccct cctaaaaggt gaagagagta tttcattaac catccctaac
     7141 aatgatctga atagcagtaa aaagaaaggc aagcagagtc cttcctttaa acctgaaagt
     7201 aacccattat ttgaaaaact tcgtgcgtta agacgtcagt tggccgatga agaaaacaaa
     7261 ccaccattta tgatattcag tgatgctaca ttacatgaaa tggcccaatt aaaacccaaa
     7321 aacagtaagc aactactgaa tgtatctggg gttgggcaac ataaattagc tcgttatggc
     7381 cattattttc tcgaagcctt aaacgaattt tcagaagtcg agtgagattc cgttatgcgt
     7441 aaattaccaa gaccctttta cgaacgtgat accattctgg ttgcaaaaga actcttaggt
     7501 aaatatttgg ttcatcatga tggattggaa gagaagattg ggcgcattgt agaagtcgaa
     7561 gcctaccttg ggcaacatga tttggcctgc cattcatcaa aaggattgac taaaagaact
     7621 aaagttatgt ttgggccagc aggctatgcg tatgtttatc taatttatgg catgtattat
     7681 tgcatgaacg tagtcaccga aaaggagggg ataggttcag cagtattaat acgtgccctg
     7741 gagcctatca aaaacattca agacagaact caaggaccag ggttattatc caaagctatg
     7801 cgcattgact ccaaactgaa tcatcgtgat ttattaagca atgattttta tattgctgag
     7861 ccaaacagtc caactgattt taccattata gaaaaaccca gagttggggt tcattatgct
     7921 aaagaatggg ctaatgagtt attaagattt tatattaaag acaatcctta tatttcgaaa
     7981 acctgattag gcctcgtgca taaactgtct ctttcatttt attgccgcat tgcaaagatt
     8041 tctgtgatgc tcagggtatg taaaaagcgt attatcgtat taatgagccc attattatta
     8101 catccctttc ttcaagcatg tcctgtcaat ccccggatcc aggctttgtg cctaatccgg
     8161 ggtaatttct tgaaataact ctactctaac ccaattgcct tgggcgggca gatataggca
     8221 ttacccagat tagtcacatt ggtttgttct gaaccgccgc tcataaaacc accgtgacga
     8281 tcaaatgaac cactcatgga acccgttaca ccggctcggt tcgtcaccaa ttgaatgtaa
     8341 tttgcccctt ttttgcttgc tttattcttc agatcattca ttgctccttc ttccaaattg
     8401 cgattggaag taaagcctcc ggtaaagaaa ttaccctgat tgcctacaac ctggcccacg
     8461 tatttgcaac ctttaggtgc cggattcggg cttgcaatga cacgttcggc ctgaggatct
     8521 gctggaatag ctgcgcatcc ggtaatcatc aaagtaataa cagacgcaga tagtagtttt
     8581 ttcataatta cacctttttg ttatcaacaa aacaatgaaa tgttaacaac tcctgtttga
     8641 ttaaacatta caacccactt ttaaatgcac ttgataatat ttcactttat tatcatcaat
     8701 gaaacctctt gtttctacta cttcaaacca atccaatttt ccatgcgttt tagctacttg
     8761 ctttattgca ttatcaatcg ctttttcaat tccttcttgc gaagtcccaa ttaactccac
     8821 gatttggtaa actttatctg tcactcttct ttccccctct attaatttgt ttctcagctt
     8881 ttgctattgc cttgcgaata gccaatgctt cttcggactg cgctggcgcc aactggagta
     8941 gccgttgcca gtaattaatc gcagcttcat aatcatgact taagtaagca tgcatcgcaa
     9001 gcatggctaa agcatccggt tgcctggggt tattttttaa taaggtatga aaaatttcta
     9061 aaatatcagg attgaactgt tgattactca attgccataa actgtgggca taattgactg
     9121 caaattgctc gtcttcaggt ttaagctggt gggcttttgc aaaagcgagg gaagcatttt
     9181 ggttatcctt ttgactgctg tataatctgc ctaacaaata ccaacccttg gcactttcag
     9241 gctgcgcatc caacttggcc ttcagcttgt cgattaattc ctgagggctt ttaatcgatt
     9301 ttagcatcga ttgcgctagc tcctgagcct catttcgatg tagatattct tgccaccgag
     9361 caaaatctcc ccaaaaatag taccctgtaa aaaccattat aaaaataatg ggggcgagca
     9421 acaaacttac caaacgatta cgccgcaaag gatagataat aagagtgcat gcaaaaacgg
     9481 ctaaaacaac caataaaata attaaccacc attcattcat gagaatttcg cttaaaacat
     9541 gtccgccaaa aaataataaa gccaatcaat aaaaacagga atgggccaaa ccacaataaa
     9601 agagtcacgc tcttaacagg cggtttaaat agtataaaat ccccataacg tgcggttaga
     9661 taatccatta tttcactatc gcttttgccc tctttcacta attcataaac ctgaaaacgt
     9721 aaatccttcg ctaattgagc atttgaatct gccaaatcct gattttggca taccaaacaa
     9781 cgcaaatctt ttaaaagatg gttaaattgt gcttcttttc ttgctgagtc caatggatac
     9841 atactacttg caaacgcaac ggatactaat agcagcaata aaatcacctt aaaacagaag
     9901 tggaatactc tcattatgat ttctccaaat tcctcattaa aggtaaaata tcagttaacc
     9961 aggttttctg attcattatc ccgacatgcc tgtagcggac tattcctttc ttatcaatga
    10021 caaaagtttc aggcgcccca tatacaccta agtcaatagc gactttacct tctgaatcct
    10081 ggcctatgag tcgataaggg ttcccccatt gtgcaagcca tgctctcgcg tgctctggat
    10141 tgtctttata atttaaacca tagataggta cgccttctct tgcgagttgc aacataaata
    10201 cttgctcttc aacgcaagca tcacaccaac ttgcccagac attaagcaaa acgacctgat
    10261 tatgaaagtt cattgcacta aaagtagaat cgggattttg caactctggt aaggcaaagt
    10321 ctggtaatgg ctttccaatt cgagctgaag gcaaatgatg aggatccaaa gataaacccc
    10381 gccagagaaa aaaacataaa agacaaaaaa tagcgatagg aataacacgc caccctattt
    10441 ttttcatatc ttgatctcct gccctgccat taccgttact tgcctttttc tgtaatacct
    10501 tctatctgtt aatgccaaaa cgcctccggc taaaatcata aatccgccag cccatatcca
    10561 gcggaccaac ggtttataat aaattcggac cgaccaggca tcctcagaca gtggctcccc
    10621 caaagcaatg taaatatctc ggaaaggagt cacatcaatc gcagactcag tcattggcat
    10681 ttgtccaatg gtatacactc ttttttcagg aaaaatttgc ctagttttat tctgatattg
    10741 tattgaaaac tcagcccttg tccctttgta atttgggcca ttcaacggag tctgattaat
    10801 gaatccaatc tgataatcca gcagactcac ttgcttgcca ggagacatac gtacatcatc
    10861 ttgtacgcca taagctgaag aaacagcaat gccaatcact gttgctgcga caccacaatg
    10921 agccaaaacc atcccccaat aagcctgtcc aactcctgcc acccctctat ctctgacttt
    10981 gtgatacgcc gcattagcag tactcaacaa aacccagaat gccaaaatta atcccaaaag
    11041 ggctgtaagg tgtatttcag ttgaggtaca gaatagtaaa atgacaggca atatcagact
    11101 aagcaaaaaa atacgattta gttttctgaa gacatccctc caacaatcat tctgccattt
    11161 caaacggaca cctaacccca ttagcaataa taaaggaatt attaatggaa caaatactgc
    11221 attaaaatag ggcgctccaa cggataattt gcctaaccct aacccttcca ccagcaatgg
    11281 ataaaccgtg cccattaata ccgtcagcat aataaccact aaaaacacat tgtttaacaa
    11341 caacgcactt tctcgggata ataagccagg atgtttattg acttgcaagg tttgggctct
    11401 gaaaagaaac aatgttaaag aacccccaat gacgaataac aaaaatccca gaatatataa
    11461 tccccgctga ggatcaaccg caaaggcatg aactgaagtt aaaactccag agcgaacaag
    11521 aaaagtacca attaaactta aagaaaaagc agcgattgca agcaacatgg tccacgcttt
    11581 aaattgctgg cgctgctctg taactgccaa agaatgcaat agtgccgtac caaccagcca
    11641 gggcataaaa gaagcgtttt ctacaggatc ccaaaaccac caaccacccc agcctaattc
    11701 tctataagcc caccaactgc ccaaagtaat tcccgcggtt aaacaacacc aggcagccag
    11761 agtccagggt ctggtccatt tagaccaact ggtttctact ttccctgccc acaaggcggc
    11821 aatagcaaaa gcaaaagcga cacaaaaccc tacatatccc atgtacaaca taggaggatg
    11881 gaataaaaag ccaggatctt gcagcaatgg attcagatca cggccctgtg tattcaatac
    11941 ttgaaattgg cgcaagaaag gattggaagt cattaacaaa aaaagaataa aaccaatgct
    12001 tagccaacct aaaataacta atacccgagc gcgcatttcc gtatcaaggc ttgagctaaa
    12061 aaaacaaacc aggatcatcc aaaaactaag gatagcaacc cataacagca tggaaccttc
    12121 atgcccaccc cacacagcac aaaatttata aaaccatggg agcaataagc tggagttatt
    12181 cagcacatag atcaccgaaa aatcatccat caaaaaacag gccgtgagac ataggtaagc
    12241 acatgccaca aataaaaatt gcagcacgac ataattcctg gaggaatcaa tccagtcttt
    12301 ccgattgaat tgcaaaccag ctaatgggat caaagctaac atcaaagaag ccactaaagc
    12361 caaaatcaaa aaaaacagtc ctaattccgc tatcattgtt tcaccttatc agctaaagca
    12421 gactttactt gaggtggcat ataattggca tcgtgttttg ccaaaacttg tatagcatga
    12481 aaatgatgat tatcagttac ttctccttct gcaactattc cttgcccttc tcgaaataaa
    12541 tcgggaagta tccctgaata ggtaacaaca atcgtgtttt caaaatctgt aattttaaac
    12601 tggacatcca aacctttttt ggcacgtact atgctgtttg cttccaccat acctccaact
    12661 cggatgtggt gttttgctgg tgcttctccc gcaacaatct gcgtgggggt gtaaaataaa
    12721 ctgatatttt gtcttaaggc atatagaacc aaggcagccg ctgcagataa aacagacagg
    12781 gcaaacagta agataaataa tttgcgtcgc cgtacagggg tcatttcacc tatctcttaa
    12841 accaacgttg taattttttg cgggtacgag ttctttgcca ctttattcct aaaatattca
    12901 tgcccaaaac cacgcaaacc aaaccataag caggccaaac atagagagaa taaccgccca
    12961 ttgccaacca ttcaaaaaac tgattcatca gactcttcct caaattgaac cttaacccat
    13021 gattgctttc tctccctaaa taagacttca ttacgtgctt tttccaaaat gatccataag
    13081 gaatacaaga aaaaacccag caaagtgatt aacaaaggat acaacatact gggagcaatt
    13141 ttgggtttgg caaacacaga taaagtcgca ccttggtgta aagtattcca ccaataaact
    13201 gaataatgaa ttattggtaa atcaattaag cccaccaaag ctaaaattgc tattatttta
    13261 tctccatctt ctttattttt taccgcttgg taggtagcca gaattgccag atagagtaac
    13321 aaaagtatta actctgaggt caggcgcgca tcccataccc accaggcacc ccacatgggt
    13381 ttaccccaaa tgctccctgt aattaaagca agaaatgcca tgcaggcacc taattgcgcg
    13441 actttatgaa tcaaaagccc tgccattttg atacgccaca ccaacaataa aatggccaga
    13501 aaccccatcc aggcatacaa tgccattgat aaaaaagcgc tgggcacatg aacaaaaata
    13561 attcggtatg catccccttg ctgataatct ggtggagcaa ataccaatcc ccaaaccata
    13621 ccaatggcta tggtagctaa agcactgact gccagccagg gaatgagacg tcccgcgtag
    13681 ttataaaaat tttttggcga tgccaactga tacaatatct tccacatagg aaacaataaa
    13741 atcaaaaaga aggattctac cacgcatcaa aacaatcaca aaacaatcac aaaatttagt
    13801 tccaaactta gggtctgttg acatttcgct cgcttgagtc gaaaaacctt gattttcgag
    13861 caccgtagcg gagcatacat agaagtatgt gagtaacgga gcacagaaaa tcaaggcttt
    13921 tcggccaaga tagtagaaat gtcaatagac
//
LOCUS       DASLOE010000073        13912 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.073, whole genome shotgun sequence.
ACCESSION   DASLOE010000073 DASLOE010000000
VERSION     DASLOE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13912
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.073"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..947
                     /locus_tag="U6309_001673"
     CDS             <1..947
                     /locus_tag="U6309_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEN5505114.1"
                     /translation="YLQKLKLGFQLLSDLPPEEQIKYLNSIYLAHIKVFPYSNFELRR
                     IANQHPVQRQSLSFFNYTKLLSSEHGGYCYQSAGLLFDALVQLGFKTEYCAARVLVGE
                     PVNSPKVLAIPATHLVLVVTLGDKKFLLDPGLGASAPRLPILITGKDESILQGKEEFK
                     FYKHDGVHVLERKTSQGWFRLMQTDLIPIDLKTVELNLLKLERYPDTISIRDNKTIVG
                     AITEYGRKSLVWDAQSNQLKFSKHDGDEVIQKTLTSFEDGVQTLVKEFDIDHISAQEL
                     KIYCTETNLPQPIKPWTVDLPLDQAELKKMEENLIFRL"
     gene            complement(1026..1754)
                     /locus_tag="U6309_001674"
     CDS             complement(1026..1754)
                     /locus_tag="U6309_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505115.1"
                     /translation="MTPSISLITSYSLLPAVLMLIGGGIASLYRPGGSITSATQHFAA
                     GVVFAAVAKELLPKIGAYHDPLALIIGFSVGILAMLFLKWLTNRLEDLEQEKTGLSWG
                     LLTAVGIDLLIDGILIGVAFLAGERGGILIAIALAIEIFFLGLSTTATLGARQVNVPI
                     RLLTSVILAILIPVGAALGASLLIHLPLSITNGILSFGVAALLYLVTEELLLEAHEVR
                     ETPYITLCFFIGFLLILLLEGLAA"
     gene            complement(1839..2519)
                     /locus_tag="U6309_001675"
     CDS             complement(1839..2519)
                     /locus_tag="U6309_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HEN5505116.1"
                     /translation="MKPIAHIADKEIISIPVKECGEELIDLKNQNIIVYGPPPETPLT
                     ANDYTKIRKTVYQKLCLAQNDLPNGWRFRLYEGLRSLTVQEILFNQQRDNLRQSKQYD
                     SYEDLFNETCRLVSPVVHLDGTANIPPHSTGAAVDLELIDSYGNLVNLGMAAKDWLIV
                     DPDLCETDSKLISEEAMQNRILMLKIMAKHGFVNYPREWWHFSYGDRLWAYMTEHSTA
                     IYGAIINN"
     gene            complement(2701..2979)
                     /locus_tag="U6309_001676"
     CDS             complement(2701..2979)
                     /locus_tag="U6309_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505117.1"
                     /translation="MSIRMITEIETLLKRAGFTYVGNGRGLGEFEKYENHLYHKTVYG
                     SIQQIQLAMDRENDTVRPVFSKNVPVELRDSIYELMKNPSEKNSLTFN"
     gene            complement(3064..3549)
                     /locus_tag="U6309_001677"
     CDS             complement(3064..3549)
                     /locus_tag="U6309_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505118.1"
                     /translation="MNRFIRFLLLLLITCSSGSFNLYAANKNNDFVLFEKIFTDWTLA
                     FNQKKLAPTCALFSKSLAADYQGAPQKNYTSICHGFKKIFQQTRKNYQYHFKLKRVYR
                     SGHLAVARITWYLQIFEQGKLISETQDEGLDVFLKDSQNQWKIINFIAYPVPDNLNEK
                     H"
     gene            3696..4310
                     /gene="mavI"
                     /locus_tag="U6309_001678"
     CDS             3696..4310
                     /gene="mavI"
                     /locus_tag="U6309_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavI"
                     /protein_id="HEN5505119.1"
                     /translation="MYSVKLCFALFRLYCYWRKLSMGLSAQQKTAIKSYFESNYNLFF
                     GIPTERKEGDLTPNEHVMVLQYLRFYLLKLPIECLEEELVNKQINGDDTVGILLEDFL
                     GNTLSPMNPDAYFHSQKNFIQMGNAEDPAKPEDIIQYLIEVIENMSQQMDHGYLKKES
                     DPRIEKSNSSYASRALKWGLFGGAALAITYAVSKLSDKSHTNSL"
     gene            4537..5094
                     /locus_tag="U6309_001679"
     CDS             4537..5094
                     /locus_tag="U6309_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2443 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505120.1"
                     /translation="MIRKSEMIRHFFIVLFFFVGFMNQASALPWFENENDLRNNNKLR
                     YRAHDEIKKNQIDFSGRWSGQCDSNPAVDLTIKHTREHISISYGFMEEKYIPGEVRAE
                     SSSRLSLSESGSTTVKWNQDNTALIFINYHLFINESGSLNVFFSKVSMALEQEQLVVK
                     GQHFQTGNTLDDIKQETISCIYDRK"
     gene            complement(5218..5628)
                     /locus_tag="U6309_001680"
     CDS             complement(5218..5628)
                     /locus_tag="U6309_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN5505121.1"
                     /translation="MKPLIPYLLFNGTCREAMTFYQNCFGGELELMTYGDSPEGACDK
                     KADKNEIMHACLRQGGFTLMASDWPGGEAKQGNNVHLNIDCSTKLEIEALFKKLSQEG
                     TVVQALADTFWGAHFGMLIDRYNIHWMLNYSLKQ"
     gene            complement(5729..6202)
                     /locus_tag="U6309_001681"
     CDS             complement(5729..6202)
                     /locus_tag="U6309_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN5505122.1"
                     /translation="MQKITPCLWFDDKAEEAVTYYISIFKNGSIEKITYYGKEGVEFH
                     KKPTGSVMTILFNLNGQQYVALNGGPEFRFNESISLMVTCDTQDELDYFWKELSADGE
                     EGPCGWLKDKFGLSWQIIPNILAELLSDHQKSEKVMRVLLQMKKLDIAKLKAAAE"
     gene            complement(6241..7200)
                     /locus_tag="U6309_001682"
     CDS             complement(6241..7200)
                     /locus_tag="U6309_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEN5505123.1"
                     /translation="MGILIDGIWHDENNIKTDKEGRFVRAESGFRQHITASGESGFKA
                     EPDRYHLYLSYACPWACRTLIMRKLKGLEKIISVSFSDPVMGKNGWSYGESGNETADE
                     INHIHYLKELYLVARKDYTGRVTVPVLWDKKKKTIVNNESSEIMRMLNSEFNAFSSTD
                     HDYYPKQWRQKIDEINQLVYENINNGVYKCGFAKSQKAYEEAFDALFNTLDQIENLLA
                     TQRYLIGEQLTEADWRLFTTLIRFDAVYHGHFKCNLQQIADYPHLSNYLRELYQFPGI
                     KETVNFNHIKQHYYRSHLSINPTGIVPKGPKLNLDEPHNRYEF"
     gene            7349..7900
                     /locus_tag="U6309_001683"
     CDS             7349..7900
                     /locus_tag="U6309_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN5505124.1"
                     /translation="MFNVAVIVGSLRKESFNRKLAFALDKLQHPTFQLSHIKIDDIPL
                     YNQDFDNNLPDSVIRLKKEITEADAVLFVTPEYNRSIPGVLKNIIDWGTRPYGKNVWA
                     GKLMAAIGTSPGAIGTAVAQSHLRSIMVAVDGVYFGQPEIYLVYKDGLIDNNFSITME
                     STRNFLQGFLDRFAREIEKHKSI"
     gene            8167..9357
                     /locus_tag="U6309_001684"
     CDS             8167..9357
                     /locus_tag="U6309_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEN5505125.1"
                     /translation="MNFIGWTERQSLFRLFPLIISISFAMDVFVPAIPEMSRYFSTDS
                     TTMQATLYLFMLTVALGQLLVGPLADRFGRRQMALSMALLFFAGSLISALAPSVPALI
                     FARIIQAAGACGTYLLCFIIVRDNFSTGTCARLFSILSGTNSMVASSAPVIGGLLLDM
                     TQDWHSGFYFLTLLGLLMSGIAFRYIPDYHYPKPEQPQMRVHQVMSRIISNSDFRQYT
                     LVASASLLGLYLFCAQSPGILITQLHLSATEYGFWFGLNAMTVFAANMMAARLTYSHS
                     LESIVRWGLVLMTLSCFFMLALNLRQCSILSFMLPMLCLTVGIGISMGTATALALKDF
                     EQQAGVATALLGACQFGLAGLIGVLTAQWMPGPFSVAIPVLCFTAVGLARITKFSFRI
                     LSGS"
     gene            complement(9716..10096)
                     /locus_tag="U6309_001685"
     CDS             complement(9716..10096)
                     /locus_tag="U6309_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505126.1"
                     /translation="MTRNSVKTVCIFLSIILYVIALCLPAAIIPDFERGIHQTHYGFE
                     ILITGWMGFLAWQPAWLANLLYLFSLLTYGSKSSYRFGTAAFLLALVSPFILVEKLLI
                     GFYVWLVSFAVLLYGNNLHEVTTE"
     gene            complement(10133..11176)
                     /locus_tag="U6309_001686"
     CDS             complement(10133..11176)
                     /locus_tag="U6309_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEN5505127.1"
                     /translation="MITNNIRFNWIIFILLIGLLNLANINELYADETGEQSQFNLESY
                     SPQTKKLCQTVAHISVPSQNQLDEASKASLKNCDALDLYYGFNAAPDYDKAFQCALMN
                     KDYNVLVMAYANGRGVTFNPDLAMHFACMMEDAAPAEIDGRIAHLAQIKEGNKKTSFD
                     ICDDITSGYMMGWCSSIDQRLEDVKRNQKINDLISQWTAQEQLLYQQVRKIAEVYIRD
                     HSMNEIDLSGTARSAFAINAQLNLNQRLFELLQKVNRCETPLMTIKQYEEMDKQLNNI
                     YKKLMADTSSFQYTTVTKEGIKKTEIAWIQYRDAWIQLARVKCPKISAESWQVLLIQD
                     RIKLLNEILELAE"
     gene            complement(11203..11697)
                     /locus_tag="U6309_001687"
     CDS             complement(11203..11697)
                     /locus_tag="U6309_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2434 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505128.1"
                     /translation="MTIMMNKDKHSLLAIAAAEVPPRTKPSIYPEPFASMMTGRQKHA
                     LGDFFGIKNFGVNLTRLAPGAQSALLHKHKLQEEFVFILKGQPTLVTETADIQLHPGM
                     CAGFTPDGTAHQLVNRTADEVVYLEIGDRTQGDEVNYPADDLAAVFGNDGQWHFMHKN
                     GMPY"
     gene            complement(11964..13766)
                     /locus_tag="U6309_001688"
     CDS             complement(11964..13766)
                     /locus_tag="U6309_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505129.1"
                     /translation="MEIYMAPLFPEKNAPLTIKQGPVDDCYLLVALDCIFNSGDEARQ
                     LLASKFTQTPLGLTLRIKRNNQSTRLDSQKMRGKYIHVYDRNTDEDVFFIGQQKLKEI
                     DNSKKGVETNSLAIKVLERISSYYYTGDWDHTNESGSLTAHSIRQRHMETSTRFVGKL
                     LGIHAEDTEEMDAIIKLKLMNPKEAVYISLAYGEPDANGKIHDRHALRIDKVVPKING
                     GYDFILVNPWDNQKQETFSLEELEKRDCRFCTFSLNQQRHEITRILLKNPIGQGQYIF
                     ANPELFTLLLQMQKQKQSLLSDAKNIQACINLHKQMPYLPSLYNSLTSDEQKKLIQGM
                     VGTKGDKEKFIKHLIINAPRLDLIKTIFQNEPPENKTENMIVDLALEAKKKNSPARIV
                     FEDPEFFTLIMNRAIHHKATIRGCSQEQAKKLIESKMINFYFDNLGTDSLTRNARLCE
                     LFAAKIFTKTSIETWFAARLSLTNVDLSQALRTLGFSDQRSGVMSAPVLKNQTALVAG
                     QVGFFPIHKSSPAPVTNKSPAPTQELTAKKSSVPTLPSIVKKPSTPLSEPIGKKLSTP
                     IPALLVNGLSIYTPVKPSVKTLPPLKEVRLTSLP"
BASE COUNT         4121 a         2541 c         2645 g         4605 t
ORIGIN      
        1 aatatctcca aaaattaaaa ttaggttttc aattattatc tgatttaccc cccgaagaac
       61 agattaaata cttaaacagt atttatttgg cacatattaa agtttttcct tattccaatt
      121 ttgaattacg cagaattgct aatcaacatc ctgtccaaag acaatccttg tcttttttta
      181 attatacaaa gctgctttct tcagagcatg gggggtattg ttaccaaagt gcgggtctgt
      241 tgtttgatgc cttagtgcaa ttgggattta agacggaata ttgtgcggcg cgtgttcttg
      301 ttggcgaacc tgtgaactcc cctaaggtac ttgcgatacc tgctactcat cttgttttag
      361 tcgtgactct gggagacaaa aaatttcttc tggatccggg attgggcgct tctgctccaa
      421 ggctcccaat attgataaca ggaaaagacg agtccatttt acaaggtaaa gaagagttta
      481 aattttataa acatgatggt gtgcatgttc tggagaggaa aacaagtcaa gggtggtttc
      541 gcttgatgca gactgatttg atccccatag atctcaaaac ggtagagctg aacttactca
      601 aattggaacg ttatccggat acaatttcta ttcgagacaa taaaacgatt gttggagcta
      661 ttacggaata tggacgtaaa tcgttggttt gggacgcaca atcgaatcaa ttgaaatttt
      721 caaaacatga tggtgatgaa gtcattcaaa aaactttaac cagttttgag gatggagtgc
      781 aaacacttgt taaggagttt gatatcgatc atatttcagc gcaagagttg aaaatttact
      841 gtactgaaac gaatcttccc cagccaataa aaccttggac tgtggacctc ccccttgatc
      901 aagcagagtt aaaaaaaatg gaagaaaatt taatttttag actatagaaa agtgatttgt
      961 atttgttttt ccaagctggt tcagctcatc atagcttatg tttttattaa aatacctgct
     1021 tgcatttatg ctgccagccc ttctaacaat aaaatcaaca aaaatccaat gaaaaaacat
     1081 aaagtaatgt aaggggtttc acgaacctca tgggcttcaa gcaataactc ctcagtgacc
     1141 agataaagta atgccgcaac accaaatgat aaaattccat tggtaatcga tagtggtaaa
     1201 tgaattaata aactggcgcc cagagcagct cccacaggga tcaatatggc caaaatgaca
     1261 ctggttaaca gacgaattgg cacgttcact tgtcttgccc ctaaagtggc ggtcgtcgat
     1321 aatcccaaaa agaaaatttc aatggcaaga gcaatagcga taagaattcc tcctcgttcg
     1381 ccagccaaaa aagcaacccc aattaagata ccgtctatca ataaatcaat tccaactgcg
     1441 gttaataatc cccaggatag acctgttttt tcttgttcaa gatcttcgag cctgttagtc
     1501 aaccatttta gaaataacat ggccagaatg cctactgaaa aaccaataat aagagcaaga
     1561 ggatcatggt atgctccgat tttgggtagc agttcttttg ctacagcagc aaaaacaaca
     1621 cctgctgcaa aatgttgagt ggcgctggtt attgaaccac ctggacggta aaggcttgcg
     1681 atgcccccac caattaacat caacacggcg ggtaataaag aataggaggt aataagcgaa
     1741 atagatgggg tcataagaga tattttttaa ggtaatattg acagaataat caaataatta
     1801 gccaagttca agaatgacag gcattgtcgc attttggctt aattatttat gatagcaccg
     1861 tagatggcag tagaatgttc tgtcatatag gcccataggc gatctccata ggaaaaatgc
     1921 caccattctc tggggtaatt aacaaaacca tgcttagcca ttattttcag catcagaata
     1981 cgattttgca tagcttcttc agaaataagt tttgaatcgg tttcgcataa atcagggtcg
     2041 acgatcaacc aatcctttgc ggccattcca agattaacca gattaccata actatcaatg
     2101 agctcaagat cgactgcagc cccagtgcta tgtggtggta tattagctgt cccatccaaa
     2161 tggactactg gagaaaccag acgacaggtt tcattaaata aatcctcata ggaatcgtac
     2221 tgtttgcttt gacgcaggtt atcacgttgt tggttaaaca gaatttcctg aacagtcaga
     2281 ctacgcaatc cttcatataa tctaaagcgc cagccattgg gtaaatcatt ttgtgccaag
     2341 cataattttt gatatacagt tttacgtatt ttggtatagt catttgcagt taaaggggtt
     2401 tccggagggg gcccataaac aataatattt tggtttttaa gatcaatgag ctcttcgccg
     2461 cattctttaa cagggataga tattatttct ttatctgcga tatgtgctat gggtttcatt
     2521 atttttcgca aaacaagcaa aattttattg catggattcg acaagtcatc cgcacgttaa
     2581 ttatatcatg ctaacaaaaa agcaggttag gctttatgct ttaattattt aaaatttttc
     2641 tgataaaacc taacaatttg cagttagcca tttaaggaaa ttatggctca aactctaatg
     2701 tcaattaaat gttaacgaat ttttttcaga tgggtttttc atgagttcat aaatactgtc
     2761 acgtagctct actggaacat tttttgaaaa cacaggtctg accgtgtcat tctctctgtc
     2821 catagccagt tggatctgct gtattgagcc ataaacagtt ttatggtaga gatggttttc
     2881 atatttttca aattccccca agcctctgcc atttcctaca taagtaaacc cggccctttt
     2941 taataatgtt tcaatttcag tgatcattct aatactcatc aaataaatag gggcgtcatt
     3001 atattgataa aatacaacaa gatcaatcca agatcatatt acaatttatt gttacacctt
     3061 gttttagtgt ttttcattaa gattgtcagg gacagggtat gctatgaaat tgataatttt
     3121 ccattgattt tgtgaatctt tcagaaaaac atctaatcct tcatcttgcg tctcggaaat
     3181 gagttttcct tgctcaaata tttgcaagta ccaggtaatt cttgcaacag caagatgacc
     3241 tgagcgatag acccgtttta atttaaaatg atattggtaa tttttccttg tttgctgaaa
     3301 tattttctta aagccatgac aaatagatgt gtaattcttt tgcggggcac cctgataatc
     3361 ggcggccaat gattttgaaa ataaagcaca ggttggtgcc agtttttttt gattaaatgc
     3421 caaagtccag tcagtaaata ttttttcaaa taaaacaaaa tcattatttt tattggctgc
     3481 ataaagattg aaagaacctg aagagcaggt tatcaagaga agcaacaaaa aacgaatgaa
     3541 gcgattcata ttgattcgtg aagcccaaag aaaagattaa cttaacaaat ccttccagac
     3601 aatgacagca aattcttaat gttgttttta tttgattatt tataatttgg acataaaatt
     3661 cattgtaagg tcatcatgaa agatttaaga tcactctgta ttctgttaag ctttgctttg
     3721 cattatttag actttactgc tattggagga agctcagtat ggggttatca gctcagcaaa
     3781 aaacggccat aaaatcttac ttcgaatcca attataatct gttttttggg attccaactg
     3841 aacgtaaaga aggcgattta acacccaatg aacatgtgat ggtattgcaa tacctgcgat
     3901 tttatttatt aaagttgcct attgagtgtt tggaagaaga gttggtgaac aaacaaatca
     3961 atggtgatga tacggtaggt attttattag aggattttct gggcaacact ctttctccaa
     4021 tgaacccgga tgcttatttt catagtcaaa aaaattttat ccagatgggt aatgctgaag
     4081 atcctgcaaa gccagaagat attattcagt atttaataga agtcatcgag aatatgtcgc
     4141 aacaaatgga tcatggttat ttaaaaaaag aaagtgatcc cagaatagaa aagagcaaca
     4201 gttcttatgc gtcgagagca ctaaagtggg gcctatttgg aggagctgct ttagctataa
     4261 catatgctgt cagcaaattg tctgataaaa gccataccaa tagtctgtga aagcgacaat
     4321 attcggtttc ggggtaacgg aatatcaatt tccagcggat ttctcgaagt acgtcttata
     4381 ccaaagcaaa accatgctat gttgtaagag ttgtgtcaaa agcaaggacc ctgaattgct
     4441 attttcccgg atactcctta aaaagtagat tgcaatgata ttataaattc gttttctgcg
     4501 agacagaaaa agtattattc tgtacaaacc tgcctactga taaggaagtc agaaatgata
     4561 agacattttt ttatagtttt attctttttc gtcggtttta tgaatcaggc ttcagctctt
     4621 ccctggtttg agaatgagaa tgatttaaga aataataaca agctgagata tagggctcat
     4681 gatgaaatca aaaaaaatca aatagatttt tctggcagat ggagcgggca atgtgatagt
     4741 aatccggctg tggacctaac tatcaagcat actcgagaac acatttctat ttcctatgga
     4801 tttatggaag aaaaatatat tccgggtgaa gttcgagcag agtcatcctc tcgcttatct
     4861 ttatctgaaa gcggcagcac tacagtgaaa tggaatcaag ataacactgc tttaattttt
     4921 attaattatc atttatttat caatgaaagc ggtagtttga atgtgttttt tagtaaggtt
     4981 tccatggcat tggagcaaga gcaactggta gtcaaaggac agcattttca aacaggaaat
     5041 accttggatg acattaaaca agaaactatc tcatgtattt atgaccgtaa ataatgacat
     5101 aaagtaaatc cctgtatgct gagctttttg ctggtcacat gggacttact ttactggtta
     5161 gtgaaacgat ctcaattgat taaaaattgt cgatcagact tttttgtatt ttgatttcta
     5221 ttgtttcaaa gaataattta acatccagtg tatattatac ctatcaatca gcataccaaa
     5281 gtgtgcaccc caaaatgtat cggcgagtgc ttgcactacc gttccttcct gacttagttt
     5341 tttaaacagg gcttcaattt ctaattttgt gctgcaatca atgttgagat gcacattatt
     5401 gccttgcttt gcttcgccac ctggccaatc agatgccatc agagtgaaac caccttgtct
     5461 taagcaagca tgcatgatct cgtttttatc ggctttttta tcacaagctc cttcagggga
     5521 atcaccatac gtcattagtt ctaattctcc tccaaaacaa ttctgataaa atgtcattgc
     5581 ctctcgacag gtaccgttga aaagaaggta aggtattagc ggtttcatgt attctccttg
     5641 atagattgat tattgcaaga cttgtaactc tcaatttctt tgctaaaggt cgtttattgc
     5701 ttcgattttg tgatttttcc ttaacatctt attctgctgc agctttcaac tttgcgatat
     5761 ccagtttttt catttgcaga agcactctca tgactttttc tgacttttga tggtcactca
     5821 gcaattcagc caagatattg ggaattatct gccaagatag tccaaatttg tctttgagcc
     5881 agccacaagg accttcttct ccatccgctg agagttcctt ccagaagtaa tccaactcgt
     5941 cttgtgtatc gcaagtgacc atcagtgaga tggattcgtt aaatcgaaat tcagggccgc
     6001 cattaagtgc cacgtattgt tgtccattca aattgaagag gattgtcatc acactgcctg
     6061 ttggtttttt atggaattca actccttctt tgccgtagta agtaattttt tcaatactcc
     6121 catttttgaa aatcgagata taatacgtca ctgcttcttc ggctttatca tcgaaccata
     6181 gacaaggagt gattttttgc atggtttttt tatccttatt tctagctatt gaaaaattta
     6241 ttagaattca tagcggttat gtggttcatc caaatttaat tttgggccct tgggaacaat
     6301 tcccgtagga ttaatgctta aatgacttcg ataataatgt tgtttgatat gattaaaatt
     6361 tacggtttct ttgataccag gaaattgata aagctctcgt aaataattgg ataaatgcgg
     6421 atagtctgca atctgctgca agttgcattt gaagtgacca tgatacacgg catcgaaccg
     6481 aattaatgtt gtaaataaac gccagtcggc ttcggttagt tgctctccta tcaaatatcg
     6541 ctgtgttgcc aataggtttt ctatttgatc taaggtatta aataacgcat caaatgcttc
     6601 ttcataagct ttttgggatt tagcgaagcc gcatttgtaa acaccgttgt taatattttc
     6661 atagacaagc tgattaatct catcaatttt ttggcgccat tgttttggat aatagtcatg
     6721 atctgtagaa ctaaaggcat taaattcgct attcaacatg cgcatgattt ctgatgattc
     6781 attattgact atcgttttct tttttttgtc ccataaaaca ggaactgtta ctctaccggt
     6841 ataatctttt ctggcaacca aatacaactc tttgagataa tggatatgat tgatttcatc
     6901 ggctgtttca ttacctgatt cgccgtaact ccagccattt ttacccatga ctggatctga
     6961 aaaagaaaca gaaataattt tttccaatcc tttcagtttt ctcatgatta acgttcggca
     7021 tgcccaggga caagcatagg acaggtataa atgatatcga tcaggctctg ctttaaaccc
     7081 actctctcca ctcgcagtaa tgtgctgacg gaaacctgat tcagcacgaa caaaacgtcc
     7141 ttctttgtcc gtcttgatat tgttttcatc gtgccaaata ccatcaatta gtattcccat
     7201 tatgaattcc ttatatatca ataacttatt aatatttttt gaatactgac gcagcatgaa
     7261 tacgtcttgt ctaataactg ctttttaatg cataatcatt gataaatcga ttaattcatt
     7321 ttgtatcaat aaatttaagg aaaaatttat gttcaacgta gctgttattg tcggaagcct
     7381 tcgtaaagag tcttttaatc gtaaattagc atttgctctg gacaagcttc agcatcctac
     7441 atttcagcta tctcatatta aaatagatga tattcctctt tataaccagg attttgataa
     7501 taatttacct gactccgtca ttcggttaaa aaaagaaatt actgaggcag atgccgtttt
     7561 attcgtgaca cctgaatata accgttcgat acccggtgta ttgaagaata ttatagattg
     7621 ggggaccagg ccatacggaa agaatgtatg ggcaggcaaa ctcatggcag ctattggaac
     7681 gagtccggga gctattggaa ccgctgttgc tcaatcgcat ttgcgaagca taatggttgc
     7741 agttgatggc gtttattttg ggcagcctga aatttatctt gtttataaag atggactgat
     7801 tgataacaat ttttctatta ccatggagtc tacccgaaat tttctgcaag gatttctgga
     7861 tcgttttgcc agggaaattg aaaaacataa atcaatataa gtatgtcgac tggttaccca
     7921 aaacaatgtt tctggtaatt ggtgcatttt tctatgttcc cgatcattca ttttggctaa
     7981 agcctcttgc tcacttcgtt gttccttatg ttttaaggta gtccataatt ttagcgtttg
     8041 gactaccaat aattaattga ataacatctg ttgaatcagc aattttataa cattgagtgt
     8101 aaatctgtta caattaagcg cagtttatat ttttcataag taatatttta acaggaacac
     8161 cattaattga attttattgg ctggacagaa agacaaagtc tttttcgatt atttccactc
     8221 attatcagca tttcttttgc tatggatgtt tttgtgcccg caattccgga aatgagtcgt
     8281 tatttcagta ctgacagtac aacgatgcaa gctacccttt atttgtttat gctcactgta
     8341 gctttgggtc agctattggt cggacctttg gccgatcgtt ttggacgaag acagatggcc
     8401 ttgagtatgg cacttctttt ttttgcagga tcgttgatct ctgcgttagc tccgtcagta
     8461 ccggcattga tttttgcacg tatcattcag gcagctgggg cctgtggaac ctatttacta
     8521 tgctttatta tcgttcgaga taatttttca acgggcacct gtgcccgctt gtttagtatc
     8581 ctgtctggga cgaattcgat ggttgccagt tctgctccag tcattggggg attattgttg
     8641 gatatgacgc aagattggca tagcggattc tactttttaa ccttgcttgg gttattaatg
     8701 agtggcatag ccttccgtta catccccgat tatcactatc ccaaacctga gcagccccag
     8761 atgagggtgc accaagtgat gagccggata ataagtaact ctgactttcg ccaatacaca
     8821 ttggttgcat cagccagttt gctgggattg tatttgtttt gtgctcaatc accaggtatt
     8881 ttgatcacac aactgcacct gagtgcgact gaatatggtt tctggtttgg tttgaatgca
     8941 atgactgtgt ttgctgccaa tatgatggcc gcacgactta cctattctca ttcattggaa
     9001 agcatagtgc gttggggctt ggttttaatg acactgtcat gcttttttat gctggctctt
     9061 aatcttcgtc aatgcagcat tttaagtttt atgcttccca tgctttgctt gactgtgggt
     9121 attggtatta gtatgggcac agcaacagcg cttgctttaa aggattttga acagcaagct
     9181 ggtgtcgcta ccgctttgtt gggtgcttgc caatttggtc tcgcgggcct gattggtgtt
     9241 ttgacagctc aatggatgcc tgggccattt agtgtagcta tccccgtact ttgtttcact
     9301 gcagtcggat tggctagaat caccaaattc agttttagaa tattgagcgg ctcttaatct
     9361 tgtccgcagg caattggcca aaaaagaccg attaccgggt atgcggtagg taacactgta
     9421 accttcagtg ttgtttatcc tgcatgtggt tctgtattaa attttagtgg caatttcgct
     9481 aaaaaaggaa acatcattga taccatttct atcttgactc atcaagcgca ggatatcact
     9541 catgaaggtc ctggcgctcg tttcattgga catgatcgct ttaaaaaagt agctgcatcc
     9601 aatacataga atgatttttc caagcaagct tacaataagc ctcatgtcaa gaaatgaggc
     9661 tttaatgatt ctgtagatgt tctatagaca aggaatgaat ttggtaatct taaatctatt
     9721 ctgtagtcac ttcatgcaaa ttattcccat acagtaggac agcaaaacta acaagccaca
     9781 cgtaaaaccc gattaataat ttttctacaa gtataaaagg tgaaaccaat gccagaagaa
     9841 aagcagcggt accaaaacga taactgcttt tagagccata ggtaagtaat gaaaatagat
     9901 acaaaaggtt tgccagccaa gctggttgcc atgctagaaa acccatccac cccgttatta
     9961 agatttcaaa tccataatgt gtttgatgta tccctcgttc aaaatcaggt attattgccg
    10021 caggcaaaca tagggcaatg acataaagga ttattgataa aaagatacat actgttttaa
    10081 ctgaatttcg agtcattgat tactcgcttt ctttgataga aagtaacatt atttattcag
    10141 ctagctcaag aatttcattg agcaatttaa tgcggtcttg aatcaaaagc acttgccagc
    10201 tttccgcgct aatcttgggg catttcacac gagccagttg aatccaggca tccctatatt
    10261 gaatccaggc tatctcggtt tttttaatgc cttctttggt aaccgttgtg tactgaaagg
    10321 atgaagtatc tgccatcaat tttttataga tattattcaa ttgcttatcc atttcctcat
    10381 attgctttat tgtcataaga ggagtttcgc atcgatttac tttttgtaac agttcaaaca
    10441 agcgttgatt cagattcaat tgtgcattga tggcaaatgc gcttctggca gttcctgata
    10501 aatcaatttc attcatcgaa tgatcacgga tatacacttc agcaattttt ctaacctgct
    10561 gatataataa ttgttcttga gcagtccact gactgattaa gtcattaatt ttttgattgc
    10621 gttttacatc ttccaggcgc tgatcgatag aagagcacca acccatcata tacccactgg
    10681 taatgtcatc acaaatatca aaagaagttt tcttattacc ctcttttatc tgtgcgagat
    10741 gggcaatacg gccatctatt tccgctggag cggcatcctc catcatgcag gcaaaatgca
    10801 tggcaagatc aggattaaat gttactcctc taccattggc gtaagccata accaggacat
    10861 tataatcttt attcattaat gcgcattgaa aggctttgtc ataatcgggg gcggcattaa
    10921 aaccataata gagatcaaga gcatcacagt tttttagtga ggctttactg gcctcatcaa
    10981 gctggttttg gcttggtact gatatatgag ctactgtttg gcataatttc tttgtttggg
    11041 gagaataact ctctaaatta aattgacttt gctctccagt ttcatctgca taaagttcat
    11101 taatattggc taaatttaat aaaccaatta atagaataaa gataatccaa ttaaatctta
    11161 tgttgttagt tatcataatt ttttctttct aattatttgt tattaatacg gcattccatt
    11221 tttatgcata aaatgccatt gcccatcatt accaaagact gctgccagat catcggcagg
    11281 ataatttact tcatcgccct gggttcgatc tcctatttca agataaacca cttcatcagc
    11341 tgtccgattg accaattgat gtgctgtgcc atctggagta aatccagcac acattccggg
    11401 atgtaattga atgtcagcag tttcagtgac taatgttggc tgccctttga ggataaaaac
    11461 aaactcttcc tgtaatttat gtttatgtag caacgcagat tgtgctccag gagctaatct
    11521 tgttaagttg acgccaaaat tctttattcc gaaaaaatcc cctaatgcat gtttttgacg
    11581 accagtcatc atggatgcaa acggctccgg ataaatggaa ggtttggtgc gagggggaac
    11641 ctcagctgct gctatagcaa gcaatgaatg tttgtctttg ttcatcatga tcgtcaattt
    11701 gagttctatc cttgcaatat ttaatcaata ttagcataaa gtaaattatc ttgaatccct
    11761 actttcagga attctagatt atggatttgc tactgaattt agcgaaacca aagcaagatt
    11821 aatcttaggc aacaggaagt aaactataga gaaagatttt gagtgagtta aatttttcta
    11881 tgaatttgtt tccagatctt tgctttagga gtttcaggct aaaaaattaa ttatcagcca
    11941 gtacaataga tatatcatga actctagggg agtgatgtaa ggcgcacttc ttttagagga
    12001 gggagcgttt ttacagaggg tttcactggg gtataaatgg ataatccatt cacgagaagt
    12061 gctggaattg gtgtagataa tttttttccg ataggttctg aaagcggagt agatggtttt
    12121 tttactatag atggaagagt aggaacagat gatttttttg cagtcagctc ctgggttgga
    12181 gcaggagatt tatttgttac tggtgccggt gaggatttat ggattgggaa aaatccaacc
    12241 tggccagcca ccaaagcggt ttgatttttt agaaccggtg cgctcattac tccagaacgt
    12301 tgatcagaaa atcccagagt tcttaatgct tgagacaaat caacattagt caaagaaagt
    12361 ctggcagcaa accaggtttc aatggatgtt tttgtgaaaa tctttgcggc aaataattca
    12421 catagccttg catttcgagt cagactatct gttcccaggt tatcaaaata aaaattaatc
    12481 attttggatt caatcaattt ttttgcttgt tcctgactgc atccccgaat ggttgcttta
    12541 tgatgaatcg ctcgattcat gattaaagta aaaaactccg ggtcttcgaa aacaatgcgt
    12601 gctggtgaat ttttcttttt tgcttcaagt gcaaggtcaa caatcatgtt ctcagtttta
    12661 ttttcaggag gctcgttttg aaaaattgtt ttaatcaaat ccagtcgagg agcgttaatt
    12721 atcaggtgtt ttataaattt ttctttatcc ccctttgtac ctaccattcc ttgaatcagt
    12781 tttttttgtt cgtcagatgt taatgaattg tatagggagg gcaagtaagg catttgtttg
    12841 tgaagattaa tacacgcttg aatattttta gcatcggata acaagctttg cttttgcttt
    12901 tgcatttgca agagcaaggt aaacaattcc gggtttgcaa aaatatattg accttgccct
    12961 atcggatttt ttaataaaat gcgtgtgatt tcatgtctct gttgattgag actgaaggta
    13021 caaaaacggc aatctcgttt ttcaagttct tctagactga atgtttcttg cttttgatta
    13081 tcccatggat tgactaaaat aaaatcataa ccaccattta ttttagggac cactttatct
    13141 attcttaacg catgacggtc atgaattttt ccattggcat ctggttcacc ataggctaag
    13201 ctgatataaa cggcttcttt ggggttcatt aatttgagtt tgatgattgc atccatttct
    13261 tcagtatctt cagcatgaat acctaataat ttgccgacaa aacgggtgga ggtttccatg
    13321 tgtctttgtc ttatgctatg agctgtcaga cttccagatt cattggtatg atcccaatct
    13381 ccggtgtaat aataggagct gattcgttct aaaactttaa tagcgagtga attggtttct
    13441 accccttttt ttgaattatc aatttctttg agtttttgtt ggcctataaa aaaaacatct
    13501 tcatctgtat ttcgatcata aacgtggata tatttaccac gcattttctg agaatcaagt
    13561 cgggtactct gattatttcg tttaatgcgg agagttagtc caagtggagt ttgtgtaaat
    13621 tttgatgcta acaattgacg cgcttcgtct ccagagttaa aaatacaatc taaagcaact
    13681 aacaggtaac aatcatcgac tgggccttgt tttatagtaa gaggagcgtt tttttctgga
    13741 aacaaaggcg ccatatagat ctccaattat acaaaaagtt tttaactttg ccattttatc
    13801 aaagaaatat tatgttaata ttaagaaaag tcgaggcaag aatggtttaa acccatcatt
    13861 ttcttatgtt caggacttct aaataactcc catcatctaa ttgatatttg ga
//
LOCUS       DASLOE010000074        13880 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.074, whole genome shotgun sequence.
ACCESSION   DASLOE010000074 DASLOE010000000
VERSION     DASLOE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13880
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.074"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(323..1096)
                     /locus_tag="U6309_001689"
     CDS             complement(323..1096)
                     /locus_tag="U6309_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN5505130.1"
                     /translation="MNFLNNKIVMITGASSGIGQACARLFAAQGARLILAARRVERLH
                     ELSSELKHHNNQEHYILPLDVCKKDLVRQQIESLPGQWNSIDVLINNAGLALDTLPLH
                     QGILEHWDTMIDTNIKGLLYISRLILPGMLERNSGHVINIGSIAGHECYPNGNVYSAT
                     KHAVHAISKSMRLDMLGSAVRVTEIAPGAVETEFSEVRWKDKQRAIDFYKDFQPLMAE
                     DVADAVLYCATRPLHVDIEQMIIMPTVQASANHLSRKGR"
     gene            complement(1204..1887)
                     /gene="lem2"
                     /locus_tag="U6309_001690"
     CDS             complement(1204..1887)
                     /gene="lem2"
                     /locus_tag="U6309_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem2"
                     /protein_id="HEN5505131.1"
                     /translation="MVIIGTLMSKERIKKTALEIVEYIHGNIKACNIPNQKKENKFFA
                     PLVYLCQTECDKILSNTKISLLERLLKCAKIIADLQSGNCMQQTFLAFQRLLTRLIED
                     KLSNFSTCIPISIMTIANHAFLIIDNDIVCDPWLNFVGDLKDYCFANMKRKEYFGIRS
                     DWTCFTNTEVYDEGSTEFTIQFVNALGVGISGFSKVKLANQLMQSSSMASDLGADPVI
                     SVPREFHRI"
     gene            2124..3248
                     /locus_tag="U6309_001691"
     CDS             2124..3248
                     /locus_tag="U6309_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5505132.1"
                     /translation="MFTKSCAKATDQNRQELFMDGLLGDFQKMKEKALNQQQEKRSKK
                     RELHCKSSEKTKKPNQYAALTHSQVQEVKAKARTVNHKFHLNAEEKKLWELILLGNQL
                     AQNISSCDLPTDNEDDASLVKLTQIFADETLERTDLTWLNKILKIALYSRGSGFGNCQ
                     EKAFFAFALLLHQAQKPESLIHSLRLATFNNHFILIVNERFLMDPWLNLAFPLSNGNQ
                     QLEIDYVFEGFGRLVNYFSINQEGQCFTHSVREGGTTERDPSSEKDMTNCIHSLLGHR
                     DYFDLSIVSEREIHTKTKRRKYETQEQEVPQSRQETSNQTTEAVYIDSNHNSWDNEGA
                     ADDFLEHFEQTYPVYNPGFFSFGQEDVGLKPDLPKNLFKF"
     gene            complement(3353..4564)
                     /locus_tag="U6309_001692"
     CDS             complement(3353..4564)
                     /locus_tag="U6309_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HEN5505133.1"
                     /translation="MFSQSQQKIVCVLYDDPKGGFPPNYARESIPELKQYPDGQSLPT
                     PDSIDFIPGEMLGSVSGELGLRQFLESNGHQLVVTSDKDGSDSVFARELKEATVVISQ
                     PFWPAYLTRARIESAPKLKLAITAGIGSDHVDLQAAMEHNITVCEVTYCNSISVAEHT
                     VLMILALVRDFIPQYNTVIDGGWNIADCVSRSYDLEGMQVGCVAAGRIGLAVLRRLKP
                     FAVKLHYTDRHRLPLQLEQELNLTYHPSVESLVKVCDVVSIHCPLHPETEYLFDDRLI
                     KQMKRGSYLINTARGKICDQHAVADALESGHLAGYAGDVWFPQPPAKNHPWRSMPNHA
                     MTPHTSGTTLSAQARYAAGVREILECWLGNKPIREEYLIVSQGRLAGVGSHSYSAGNT
                     TTGTEQAVELV"
     gene            4771..5547
                     /locus_tag="U6309_001693"
     CDS             4771..5547
                     /locus_tag="U6309_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5505134.1"
                     /translation="MSEREWPANDYAIGSYIQSTVAEEYLSYLKTNPTDKILDIGCGN
                     GAFTKNILLQVPQGSVLGIDASENMLHLAQDMSEKFPNFSVQKADVLTMTFNSQFDYV
                     VSFWCLQWACANIQKAFLNIINALKTGGKFLTLFPAGDDPFIMSYYALRKSGQFASLH
                     NFIPPVDYSHLNNLEKKLESLTCRDLNVKLCRQSITLPSLDTFRKFVNGIAFYQGQLP
                     NEEIKQINEAMVRYYDSECQTKYHGEYQFNFTIYLVTGEK"
     gene            5615..6799
                     /locus_tag="U6309_001694"
     CDS             5615..6799
                     /locus_tag="U6309_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN5505135.1"
                     /translation="MHLKRTLSLTLLAFYGLGTILGAGIYALVGEVAKEAAQFTPLSF
                     LIASIIALFTAISYAELSSRLPHSAGSALYVRRAFHQKWLSGLIGWIVVLTGIISAAT
                     LSHGFVKYFQLFLPLPPSLIITILTVILAGVAIWGIRESATLILFMTLMEVGGLLMII
                     YYGRYSFASIDIKSFSFPHSFDGVLIGAFIAFYAFIGFEDMVNTAEETINPEKTLPKA
                     IFLALISATILYIAVAWVIVLTIPSNTLVKTDMPLIEIIKLQGQSPLLFSIIALISIT
                     NGILVQIIMASRLIYGMANQQNAPQIFSSVYSKTQTPVHSTLLVFLMILLFAYALPIT
                     TLAKLTSSIILCIFIIIHASLIKIKLTEKKPTNAISFPIVFPMISILLTLAFLIIQFF
                     LK"
     gene            complement(6810..8822)
                     /locus_tag="U6309_001695"
     CDS             complement(6810..8822)
                     /locus_tag="U6309_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505136.1"
                     /translation="MTKKHLNPDSQTDLGDTEKVELEDTDSVGIPTEGDLSQSKMKFI
                     WEGDFSSYQLKAADLRPITRHYKQKNHIQIVINGEHSFGQELISKKLAAMDEQINGLN
                     NDSLKYALYIQKHWLQPGYLGDQFCISNMFGSDFSKVYKELKQLLAPYLTNDGEIKKG
                     LSTQELLDLNEIVKAKFAALFDAKITRYIAFLDESKSDVCLDKQEVIDEIKKIQSGLK
                     PGQLAGYVYTNNRRVGESHFEVVIISPETIIKPVHWGNQGRERGLYSEDLPGMFDTKI
                     DSFVQTTSAIPLPQAGHEECGTLGIMYLNELLKDNSKQLRENTMLFSFYDKNNSLAHF
                     FFPSPQVLRFSQSSAYNSVIKAMLEDAPDVEVIHGNKKYRVKTLRGILEESIEAAKKC
                     GNSELAARNQKILDDLPSFRKHWLQSYDLCEQKRSLMEDRHNLYLAYTTQRMRRKADE
                     QDECADLQADFELENLQSLSESPPEQEIVTEATNTAKEEIKQSSSKDEQGFTPQQLTI
                     IIEAMQKAVTSMGTSHFVSGRDDKEKLLVHFKSAFENATNGQERHEALRNFVTAASTP
                     RQGLLNFFPAAYGKTHSAIAFYEHIKNQDPSLVEVLKTMDGKKGIKVLPQSNSSEETA
                     ATIPADQARGIMQHFKRAIQAERSSELKTGNDSVDSQNLCDTGLKI"
     gene            9137..10018
                     /locus_tag="U6309_001696"
     CDS             9137..10018
                     /locus_tag="U6309_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN5505137.1"
                     /translation="MDIRDIKSFLAVIEYHSLTLAAKKLHVTQSAMSKRIRKIEDELN
                     VRLIISEGSKLIFTEAAKQLIPYARQMFSAHQNMVQTLKHSQQFTQSIIIGASVYVSH
                     YVLPSFFKYLKETNPAIQAHLKTISEKEVEYILNHGTVDLAICPAREVSEKLLGDSLL
                     WEEKLNLVVAANHELAHRTSELSLSELSEYPAILTEKGAALRDKLDTLFEKNQLVLNL
                     GFEMSTIDAIKSLVEYGLGWSYLPDSLLSEKLKILKIKDVEITIPFHAFYLKKRISDR
                     LINDLLCDFKKWQNRDN"
     gene            10191..11354
                     /locus_tag="U6309_001697"
     CDS             10191..11354
                     /locus_tag="U6309_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HEN5505138.1"
                     /translation="MKIESFQYIQNQGWSIPTFPALDSENTLILVFASPIFRANSKPI
                     EELAQAYKKSKIIGCSTAGEIFGPNIFDNSISVAVVQFESTKLQLAKTEVKSTEESYE
                     AGVHLSKSLHQKQNPQSIFVLSDGLNVNGSELIKGLNQHAGKGVIITGGLAGDGADFK
                     NTWIIYDGKIVDHCIGAVGFYGDQIHVGHGSRGGWDIFGPERVITQSKANILYELDNQ
                     PALKLYKTYLGDRASELPAAGLLYPLAIRNPSAPDSQHIVRTILGVNEEEQSLIFAGD
                     LPKGYYAQLMRANFDRLIVSANEAGENANDRLFKNGNLNTQPVLAIDISCVGRRLVLG
                     ELTEEETETTLQSLPPKSIQIGFYSYGELSPLATGTCELHNQTMTITTLLESP"
     gene            11377..12786
                     /locus_tag="U6309_001698"
     CDS             11377..12786
                     /locus_tag="U6309_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEN5505139.1"
                     /translation="MTEMHRLLQRQLKKFGFTADGLPQNLESWTQFIAHINKTYYEND
                     QDRYLLERSIDISSKEMRDINSKLEYAQDIAQMGYWHYDADTDTATWSKGLFKILGLP
                     PSNVALNYSSFLDLVHPNDKAGLKNVVEKALKEKEDYDYELRVRNADNDYKWYRTIAR
                     ANPHKNEISGVLMDISKRKIIEEQVHELNNKLVSTARLAGMSEVATSILHNVGNILNS
                     VNVSAIILKKNISKIHYKKLSAIKTMIDENINQLSEYLLQNPKGKLILPYLDALSKTL
                     HEEYEENMKEANSLETNINHIKEIIAMQQSLSGLSNLTEKISIHDVILNAVKSVILDK
                     HQIDVRIEIEPDINEFITDKSKLFQILTNLLANAKDSVLLHEANDSRKIVISIGRTEK
                     NHIMIKINDNGIGIQKDNLDRIFSFGFTTKKNGHGFGLHCSAIYARDLGGTLYAESNG
                     KGAQFVLDLPINPKTLSPR"
     gene            12789..>13880
                     /locus_tag="U6309_001699"
     CDS             12789..>13880
                     /locus_tag="U6309_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEN5505140.1"
                     /translation="MSTESFRILVIDDNPAIHQDFMKILNVSKNSALLNKFDAELFDD
                     ETEQLGISLPEFEIDVATQGIEGIEKVKRSLEEGRPYPLAFVDIRMPPGIDGIETIKR
                     IWAIDPQIQIVICSAYSDYSWESTVNTLGLSDNLLVLKKPFDVIAVRQLASALTQKWV
                     LARETQKHTEFLNQLVEERTESLQQSFSLLRATIESSSDGILVVDLQGKIVDYNSQFV
                     KLWNIPESIMKETSESQLIQYMSTQLLNSEQHAQHVKEFSIHIDDSSIQIYHFRNGKV
                     VECCSKPHRVGPQTVGRVWSFRDITEQTRLKEKLEYQATHDALTNLPNRLLLIDRIEH
                     AISGYARHKMKFAILFFDLDRFKMINDSLS"
BASE COUNT         4376 a         2874 c         2491 g         4139 t
ORIGIN      
        1 agcgattgtg ggtttgtcta tgatctgttc gacttaactc attctttgat aagaaaaaaa
       61 ttagtttata gagagaatag aaaagagttt aagtacaggc aacgacttcc cggtttctga
      121 gccgcaactg aaagacactg tggagattta tgtagagctt caggtttcct accctttgta
      181 ggcttacaca ccaccctcca aaacgacctc ctaatctggc actacttact ggttcagcca
      241 ggatcagtca tcacctatat tttaaataat agactaacag ccccattgtt ggcaaatttt
      301 tgcgcaatta ttcacctgat gattatctgc ctttccttga caaatgattt gcagaagcct
      361 gaactgttgg cataataatc atttgctcaa tatcgacatg cagaggtctt gtggcgcaat
      421 acaaaacagc atcagcaaca tcttcggcca taagcggttg aaaatcctta tagaaatcta
      481 tagctcgctg tttgtctttc cagcgaacct cgctaaactc tgtctcaaca gcacctggtg
      541 caatttcagt cactcgaacc gcacttccta acatatccaa tcgcatggac tttgatatcg
      601 catgaacagc atgcttggtg gccgagtaaa catttccatt aggataacac tcatgacctg
      661 ctatagaacc gatattaatg acgtgcccac tattcctttc taacatccct gggagaatta
      721 gccggctaat ataaagcaat cctttaatat tggtatctat catggtgtcc caatgctcca
      781 atattccttg atgcaaaggc aaagtgtcca gagccaaacc cgcattattg attaacacat
      841 caatagagtt ccactgaccg ggcaacgatt cgatttgttg tctcaccaaa tctttcttac
      901 agacatccag aggaagaata taatgttctt gattattatg atgttttaac tcgctggata
      961 attcatgtaa acgttcaact cgtcgtgccg ccaagatgag tcgtgctcct tgtgcagcaa
     1021 aaagtcgggc gcaagcctgt ccaatgccgc tggacgcacc tgttatcatt acaattttat
     1081 tgtttaaaaa attcatcctc tcccccatca ttatcaagta caatatattt accgttaaat
     1141 cgaaccaaaa catgaacaaa aacaacagca atagagttca ttacaatcaa gatgagcgcc
     1201 ccatcaaatt cgatgaaact ctctggggac agaaatcaca ggatccgctc caagatcaga
     1261 agccatactc gatgattgca tcaattgatt tgccaactta actttactga aacccgatat
     1321 ccctacccct aaagcattta caaattggat tgtaaattca gtagagcctt catcatagac
     1381 ctctgtattg gtaaaacaag tccagtcgga acgtattcca aaatactcct tccttttcat
     1441 gtttgcaaaa caataatctt tcaaatctcc cacaaaattt aaccaggggt cacacacgat
     1501 atcgttatca atgattaaaa aggcatggtt ggcaatggtc atgatagaaa ttggaatgca
     1561 agtggaaaaa ttagatagct tatcctcaat caatcgcgtc aataaacgtt gaaatgctaa
     1621 aaaagtttgc tgcatacaat tccctgattg tagatcagcg ataatttttg cgcattttaa
     1681 aagacgctcc aacaaactga ttttggtgtt actcaaaatc ttatcgcatt ctgtttgaca
     1741 tagataaacc aaaggcgcaa aaaatttatt ttcttttttt tgattgggga tattacacgc
     1801 ttttatgttg ccatgaatgt attcgacaat ctccagtgct gttttcttaa ttcgttcttt
     1861 gctcatcaat gttccaatga taaccacagg tggtttttct tttgttatct attatacctt
     1921 ttttaaatca aaaatcgctt acaatttgac aaaaacatat cctgatagga taatttaaac
     1981 gcaagaatta ctattatctt aataattaac ttataaaata gcagtctcag tgaaccaggc
     2041 tctgttttta cagcagccca gctcattatc atcagaggtt caacttgtca tatcgtaatt
     2101 aactattata aagagagtgt aacatgttca caaaatcctg tgccaaagcg actgatcaaa
     2161 atagacagga attatttatg gacggccttt taggtgattt tcaaaaaatg aaagaaaaag
     2221 cacttaatca gcaacaagaa aaacgctcca aaaaaagaga acttcactgc aaatcttctg
     2281 aaaaaacaaa aaagccgaat cagtatgctg cccttactca ttctcaagtt caggaagtta
     2341 aagcaaaagc ccggacagtt aatcataaat tccatctcaa tgctgaagaa aaaaaactct
     2401 gggagctgat tctgcttggc aatcaattgg cacaaaacat cagttcctgt gatttaccca
     2461 ccgataatga agacgatgct tcactggtta agctaactca aattttcgct gatgaaactc
     2521 tggaaagaac tgatttgacc tggctaaata aaattctgaa gattgctctg tatagcagag
     2581 gttccggttt tggtaattgc caggaaaaag ctttttttgc ttttgcactt ctgttacacc
     2641 aggcacaaaa accagaaagc ttaattcact cccttcgatt agcgaccttt aataaccatt
     2701 ttatattaat agtcaatgaa cggtttttaa tggatccctg gttgaatttg gccttcccct
     2761 tatcaaatgg taatcagcaa ctggaaatag attacgtttt tgagggattc ggccgacttg
     2821 tcaattattt ttcaatcaat caggaaggtc aatgttttac tcattcagtg cgggaaggag
     2881 gcactacaga gcgtgatcca tcatctgaaa aagacatgac aaactgtatt cattcgctac
     2941 ttggtcacag ggattatttt gatctatcaa tcgtttcaga gagagaaatt cataccaaaa
     3001 caaaaagaag aaaatatgag actcaggaac aagaagtacc tcaatctaga caagaaacat
     3061 ccaatcaaac tacagaagca gtatatatag actcaaacca taactcttgg gataacgaag
     3121 gggccgcaga cgattttcta gagcattttg agcaaacata ccctgtctac aatccaggtt
     3181 tcttttcatt tggccaagag gatgttggtt taaaaccaga ccttccaaag aatttattta
     3241 aattttaaat catctactgt ttgccccttt ccctgaactg ccgctgtctt taaaggcagc
     3301 ggctactttg aatgaaaaca gaaagacata agacagcata agcgacgggg aatcaaacca
     3361 attctactgc ttgctcagtt cccgtagtag tattacccgc actataagaa tgagaaccaa
     3421 ccccggctaa gcggccttga gacacgatca gatattcctc cctaattggt ttgttaccta
     3481 accaacactc taatatttct ctgactcccg ccgcatatcg cgcttgcgcg gacagggtag
     3541 ttcctgatgt atgtggtgtc atggcgtgat ttggcatcga tcgccaagga tggtttttgg
     3601 ctgggggttg tgggaaccaa acgtctccag cataaccagc caaatgccca gattccagag
     3661 catcagcgac agcatgctga tcgcatattt ttccacgggc ggtattaatt aaataagaac
     3721 cacgtttcat ctgttttatt aatcgatcat caaaaagata ttctgtttcc ggatgcaatg
     3781 gacaatgaat cgatacgaca tcacaaactt tcaccaagga ttcgacagaa gggtgataag
     3841 tcaaattcag ttcctgttcc aactgaagcg gtagtctatg tctgtctgtg taatgcagtt
     3901 ttacagcaaa tggctttaaa cgtcttaaaa cagccaatcc aattcggcct gcagcaacac
     3961 accccacctg catgccttcc aaatcataag aacgactgac gcaatccgca atattccacc
     4021 cgccatcaat taccgtgttg tattgtggaa taaaatcccg caccaaggct aaaatcatca
     4081 gcacggtatg ctcagcaaca ctgatgctgt tacaataagt gacctcacaa acggtgatat
     4141 tatgttccat ggctgcttgc aaatcgacat gatctgagcc gatacctgcg gtgatagcga
     4201 gcttaagctt aggtgcactt tcaatacgag cacgagttaa ataagcgggc caaaaaggtt
     4261 gggaaatgac gacggtagct tcttttagct ccctagcaaa tactgaatca gagccatcct
     4321 tatcagaggt tactaccaat tgatgtccat tgctttcaag aaattgtctt agtcccaatt
     4381 caccactcac agaacctagc atttcacctg gaataaaatc aatcgaatca ggagtaggta
     4441 aactttgacc atcaggatat tgtttcaatt ctggaatgga ttctcttgca tagtttggag
     4501 gaaatccccc cttaggatca tcataaagca cacaaacaat tttttgctga ctctggctga
     4561 acatacctga ctccttttca tagtttgaag taaaaacatc ctgatttttt tacagcgctc
     4621 actatacttg atttaagtca ttcatctcaa taaaaatact attcataaaa gaattcatga
     4681 tctattttag ggactgtcca tgatgtatca tgaacaatga tgactgaaca attatggaat
     4741 aaataactat ctcaaaagaa aggaataagt atgtcagaac gtgagtggcc agccaatgac
     4801 tatgccattg gatcttatat tcaatcgact gtagccgaag aatatttatc gtacttgaaa
     4861 acaaatccca ctgataaaat attggatatt ggatgcggta acggcgcttt tacaaaaaac
     4921 attcttctgc aagtacctca aggctctgta ttaggcatag acgcttcaga gaatatgcta
     4981 catttagccc aagacatgag tgaaaaattc cctaattttt cagtccaaaa agcggatgta
     5041 ctcactatga cattcaactc acagtttgat tatgtcgtct ctttttggtg cttgcaatgg
     5101 gcatgcgcca atattcaaaa agcattttta aatatcatta acgcattgaa aaccggagga
     5161 aaatttctca ctctttttcc agcaggagac gacccgttta tcatgagtta ttatgcttta
     5221 agaaaatcag gacaatttgc atctttgcat aattttatcc ctcccgtgga ttattctcac
     5281 ttaaacaacc tcgaaaaaaa actggagtca ctcacctgcc gtgatcttaa tgtgaaacta
     5341 tgccgacaat ccattaccct gccatccctg gatactttca ggaaatttgt caatggcatt
     5401 gctttttatc aaggacaatt gcccaatgaa gagataaagc aaatcaacga agcgatggtt
     5461 cgttattacg atagcgaatg ccaaacaaag tatcatggtg aataccaatt caatttcact
     5521 atctatctgg taactggcga aaaataaaag aggcaacaaa taaaaaagaa aatccgttct
     5581 acgactatag atctccagat aaaatggggg agcgatgcac ttaaagcgaa ctttatcatt
     5641 aacactgcta gccttttatg ggcttggaac tatattgggc gcgggaattt atgcccttgt
     5701 cggtgaggtc gcaaaagaag cggcacaatt tacccctcta tcattcttaa tcgcatccat
     5761 catcgctctt tttactgcca tttcttatgc agaattaagc tcaaggttac ctcatagcgc
     5821 aggctctgct ctttacgtgc gtagggcgtt ccatcaaaaa tggctatctg gcttaatagg
     5881 ttggatcgtg gtcttaacag gtatcatttc agcagccacc ctatcccatg gttttgtcaa
     5941 atattttcaa ttgtttttgc cattaccacc ttctctcatc atcactatat taaccgtcat
     6001 tttagctggg gttgctattt ggggtattcg tgaatcagca actcttatcc tgttcatgac
     6061 gctcatggaa gtgggtggat tattgatgat tatttattac ggtcgttatt catttgcttc
     6121 tatcgacatc aaatccttct cttttcctca ttcttttgat ggggtgctta ttggagcttt
     6181 tattgctttt tatgctttta ttggttttga agatatggtt aataccgcag aagaaacaat
     6241 aaatcctgaa aaaacacttc caaaagctat ttttcttgct ttaatatccg caaccatttt
     6301 atatattgcc gtcgcctggg ttatcgtctt gaccatacca tccaatactt tagtcaaaac
     6361 ggacatgccc cttattgaaa ttattaagct tcagggacaa tctcccttgt tgttttcaat
     6421 cattgcatta atttctatta ccaatggaat tctggtgcaa attattatgg cttcacggct
     6481 tatttatggt atggccaatc aacaaaatgc ccctcaaata ttctcttctg tctattccaa
     6541 aactcaaaca ccagtccact cgaccttact ggtatttctt atgattttac tttttgccta
     6601 tgccctccct attactaccc tggccaaatt aaccagcagt atcatattat gtatttttat
     6661 catcattcat gcctcgctaa ttaaaatcaa actgacagaa aagaaaccaa caaacgccat
     6721 ttcattccct attgttttcc cgatgatttc gattttatta accctggcct ttttgatcat
     6781 acagtttttt ttaaagtaga ttatttctcc taaattttta acccggtatc acataaattt
     6841 tgggaatcga cagagtcatt gcctgttttc aattcgctgc tcctttctgc ctgtatcgct
     6901 cgtttaaaat gctgcatgat gcctcgagcc tggtctgctg gtattgttgc cgctgtttct
     6961 tctgatgaat ttgattgcgg taacactttg attccttttt ttccatccat agtcttcaac
     7021 acctcaacta aagagggatc ctgattttta atatgttcat aaaacgctat ggcagagtgg
     7081 gttttaccgt aagccgcagg aaaaaaattg agtaaccctt gtcttggcgt tgaagctgct
     7141 gttacaaaat tccgtaaagc ctcatggcgt tcttgcccat ttgtcgcgtt ttcaaacgct
     7201 gatttgaaat gaacaagcaa tttctcttta tcatctctgc cacttacaaa atgacttgtt
     7261 cccatactgg ttacggcctt ttgcattgcc tctatgataa tagttaattg ttgcggcgtg
     7321 aaaccctgtt cgtccttgct gctggattgc ttaatttctt ctttagccgt atttgtagcc
     7381 tctgttacta tctcctgctc tggaggtgac tcagagagag attgcagatt ctctaattcg
     7441 aaatcagcct gcaaatcggc acactcatcc tgctcatctg ccttacgcct cattctctgc
     7501 gttgtataag caagatacag attatgccta tcttccatta atgaacgttt ctgctcacat
     7561 aaatcataag attgaagcca atgtttgcgg aaagaaggta aatcatcaag gatcttctga
     7621 ttacgtgctg ccaattcact attaccacat ttttttgccg cttcaataga ctcctccaat
     7681 atacctctta acgtttttac cctatatttt ttattgccat gtatcacttc tacatcaggc
     7741 gcatcttcca gcatggcttt aatgaccgag ttataagcac tggattgaga aaaacgtaac
     7801 acttgcggcg aaggaaagaa aaaatgtgct aaggaattat tcttgtcata aaaggaaaac
     7861 aacattgtat tctctcgcaa ttgtttggag ttatccttga gcaattcatt taaatacata
     7921 atacccaatg ttccgcactc ttcatgtcct gcttgaggca gaggaatggc tgaggtagtc
     7981 tgtacaaagg aatcgatttt tgtatcaaac atccctggca gatcttcact gtataaaccc
     8041 ctttctctac cttgattccc ccaatgcaca ggtttgataa tggtttcagg actgataatg
     8101 actacttcaa aatggctttc tccaactctt cgattatttg tataaacata tcctgctaat
     8161 tgtcccggtt ttaatcctga ctgaattttt ttaatttcat ctattacttc ttgcttatcc
     8221 agacaaacat cactcttgga ttcatcaaga aatgcaatat atcgggtaat cttggcatca
     8281 aaaagagcag caaatttggc cttaactatt tcgtttaaat ccaacaattc ctgcgttgat
     8341 aatccctttt taatttcgcc atcatttgtt aaataaggtg ccaacaattg tttcaactct
     8401 ttataaacct tggagaaatc actgccgaac atattagaaa tacaaaattg atctcccaga
     8461 tatcccggtt gtagccaatg tttttgaata tacaacgcat atttgagtga atcattgttt
     8521 aatccattga tttgctcatc catggcagct aatttttttg agatcaactc ctgaccgaag
     8581 gaatgctctc catttatcac aatttgaata tgattttttt gtttgtaatg cctcgtaatt
     8641 ggccttaaat cagctgcctt taactgatat gagctaaaat ctccctccca aataaatttc
     8701 atcttacttt gactcaaatc gccttcagtg ggaataccaa ctgaatctgt gtcctctaac
     8761 tccactttct ctgtgtcacc caaatctgtt tgagagtctg gatttaaatg tttcttggtc
     8821 atgatattct cctgaataat ttgctaaaaa cttattgatt aaaatacgtt cttacaggat
     8881 acagttcgag cattactggg gtattaaaag cacatgagtc ttacgaagga ctaattccaa
     8941 aatggaatag agaaatctaa aagcagaatg taattgccaa ctacttcctg ctatgtatag
     9001 gccaagaatg ttgagtaagc actactcata tagttacacc aaatggttta acctttccat
     9061 actgttacca tctccggttt gaaaaacacc catagccgtt gtgctatact ttttataaaa
     9121 agataaagaa aatcttatgg atatcagaga catcaaatcc ttcctcgctg tcattgaata
     9181 ccattcccta accctggcgg ctaaaaaatt gcatgtcact caatcggcca tgagcaaacg
     9241 cattcgaaaa atagaggacg agttaaatgt acgtttgata attagcgagg gttccaaatt
     9301 aatttttact gaagcagcca aacagcttat cccttatgcc aggcaaatgt tttccgcaca
     9361 tcaaaatatg gtccaaacac taaaacactc acaacaattc acacaaagca tcatcatagg
     9421 ggcttcggtg tatgtttctc actacgtatt gccaagcttt tttaaatact tgaaagaaac
     9481 caatcctgcc atccaagcac atctgaaaac cattagtgaa aaggaagtgg agtatattct
     9541 gaaccatggc acggttgatt tagctatctg cccggcacgg gaagtgtctg aaaaattatt
     9601 gggcgattct ttgctctggg aggaaaaact gaatttggtt gtggcagcaa atcatgagct
     9661 agcccataga acatccgagc tttcactctc agaactatca gagtacccgg ccatcttaac
     9721 tgagaaagga gcggcattgc gtgacaagct tgatacccta tttgaaaaaa atcaactggt
     9781 gctaaacctg ggttttgaaa tgagtactat cgatgccata aaatctctag tagagtacgg
     9841 tttaggctgg agttatctgc ctgatagttt actgtcagaa aaattaaaaa tcttaaaaat
     9901 aaaagacgtt gaaataacaa tcccatttca tgctttttat ctcaaaaaaa gaatcagtga
     9961 ccgcttgatt aatgacctgc tatgcgattt taaaaaatgg caaaatcggg ataattaaca
    10021 ggaatgaatc caggacgaaa ataaccgtct ttatcaaaat cgaaacaaca gaccgttgtt
    10081 aatacgtttt tacaacacta acattgcaat agactgaatc tccgcttcct tgatcctttt
    10141 gaacatatac tataattaat cataagataa atccgagaca cagcaacatg atgaaaattg
    10201 aatcatttca atacattcaa aaccagggat ggagtattcc aacatttcct gcactcgact
    10261 ccgaaaatac cttaatttta gtttttgcca gccccatctt tagggcaaac agcaaaccaa
    10321 ttgaagaatt ggctcaagcc tataaaaaat caaaaatcat aggatgctcc actgcaggag
    10381 aaatttttgg gcctaatatt tttgataaca gcatttccgt tgcagtagtt caatttgagt
    10441 caacaaaact tcaattggca aaaacagagg ttaaaagtac ggaggaatct tatgaggcgg
    10501 gagttcattt atcaaaatca ctgcatcaaa agcaaaatcc ccaaagtata tttgttttgt
    10561 ccgatggctt aaatgtcaac ggttctgaat taattaaagg attaaaccag catgctggca
    10621 aaggggttat tattacaggg ggactagcag gagatggtgc ggattttaaa aatacctgga
    10681 ttatttatga tggaaaaatt gtagaccatt gcattggggc agtagggttt tacggtgatc
    10741 aaatccatgt aggacatggt tcaagaggag gttgggatat tttcggaccg gaaagagtta
    10801 ttactcaatc taaagcaaat attctctatg aactggacaa ccagcccgcg ctaaaactct
    10861 ataaaaccta tcttggtgac agggcttctg aattaccagc ggcaggcttg ctttacccgc
    10921 ttgctatccg taacccttct gcaccagatt ctcaacacat tgtgcgcact attctgggag
    10981 tcaatgaaga agagcaatcc ctcatttttg ctggcgattt gcctaaggga tactatgctc
    11041 aattaatgcg tgccaatttt gaccgcctca ttgtcagtgc caatgaagcg ggagaaaacg
    11101 ctaatgatag actttttaaa aatggaaact tgaatacaca acctgttttg gcaatcgata
    11161 tcagttgtgt cggcagaaga ttggtattag gtgaacttac ggaggaagag accgaaacaa
    11221 cattacaatc actgcctccc aaatcgatac aaattggctt ctactcttac ggtgaactat
    11281 cacctctggc aacaggtacc tgcgagttgc ataatcaaac catgaccatt acgaccctgt
    11341 tagagagtcc ataaaccaaa aaggaattca gtaataatga cagaaatgca tcggttgttg
    11401 cagcgacagt taaaaaaatt tggctttact gctgatggat taccacaaaa tctggagagt
    11461 tggactcaat ttattgcaca tatcaataaa acatactacg aaaacgatca agaccgatat
    11521 ttattagaac gctctatcga tatttcttct aaagaaatga gggatatcaa ttcgaaactc
    11581 gaatacgccc aggatatagc gcaaatgggg tattggcatt atgacgctga cacagatacc
    11641 gcaacatggt ccaaaggact atttaaaatt ctgggtttgc cgcccagtaa tgtggcacta
    11701 aactattctt catttttaga tcttgtgcat cctaatgata aagcaggatt aaaaaatgtg
    11761 gtcgaaaagg ccctcaaaga aaaagaagat tatgattatg agcttagagt aagaaatgca
    11821 gacaatgact acaaatggta ccgaacgata gcaagagcca atcctcataa aaacgaaatt
    11881 tccggggttt tgatggatat ctccaaaagg aaaatcattg aagagcaagt tcatgaactg
    11941 aataataaac tggtttcaac agcacgtctt gccgggatgt cggaagtagc cacatcgata
    12001 cttcataatg tgggcaatat attaaacagc gtcaatgttt ctgccattat tctgaaaaag
    12061 aatatatcca aaattcatta taaaaaactg tccgctataa aaaccatgat tgatgaaaac
    12121 atcaatcaat taagcgaata cttgttgcag aatccaaagg gtaaattaat tcttccctat
    12181 ttggacgcgt tatcgaaaac cctgcatgaa gagtatgaag aaaacatgaa ggaagccaac
    12241 agtctggaaa ctaacattaa tcatattaaa gaaatcatag ccatgcagca atcattgagc
    12301 ggcttatcga atttaacaga gaaaatttca atccatgatg tgattcttaa tgcggtaaaa
    12361 tctgtcatat tggacaaaca tcaaattgat gtccgtattg agattgaacc ggatatcaat
    12421 gagtttatca ctgataaatc caagttattt caaattctta ctaatttact agctaatgca
    12481 aaagattctg ttttattaca cgaagcgaat gattccagga aaattgttat ttccattggg
    12541 agaactgaaa aaaatcatat tatgattaaa ataaatgata atggaattgg aattcaaaaa
    12601 gataacctgg atcgaatttt ttcttttgga tttacaacca agaaaaatgg tcacggtttt
    12661 ggactgcact gttccgcaat ttatgcgaga gacctgggtg gtacacttta tgctgaaagt
    12721 aatggcaaag gagctcaatt tgtattagat ttgcccatca atcccaaaac cttgagtccg
    12781 aggtagaaat gagtactgaa tcttttcgca ttctggtcat tgatgacaac ccggcaatcc
    12841 atcaggattt tatgaaaatc ctaaatgtca gcaagaactc tgctttacta aataaatttg
    12901 atgccgagct gtttgatgat gagacagaac aacttggaat ttctttgcct gaatttgaaa
    12961 tcgatgtagc gactcaaggc attgaaggca tagaaaaagt gaaacgttca cttgaagaag
    13021 gccggccata cccactcgcc tttgttgaca taagaatgcc cccggggata gatgggatag
    13081 aaaccatcaa gcgcatttgg gccattgacc cgcaaataca aattgtaata tgcagtgcct
    13141 attccgatta ctcatgggaa agcacagtga ataccctggg tttaagtgat aatttattgg
    13201 tgctaaaaaa accctttgat gtcattgcag tacgacagct ggcaagtgct ttgacacaga
    13261 aatgggtact tgccagggaa actcaaaaac acactgagtt tttaaatcaa ttggtagagg
    13321 aacgaaccga atcattacag caatccttct ccttattgag agcaacgatt gagtcctctt
    13381 cggatggcat tctggttgtc gatttacaag gaaaaatcgt tgattacaac tctcaattcg
    13441 tcaaattgtg gaatataccc gaatccatca tgaaagaaac cagtgaatcc caactcattc
    13501 agtacatgtc aacacaattg ctcaattcag aacagcatgc ccagcatgtt aaagaatttt
    13561 ctattcacat cgatgattcc agtatacaaa tttatcattt tagaaatggt aaagtggttg
    13621 aatgctgttc taaaccgcat cgcgtcggtc cgcaaacggt aggccgtgta tggagcttca
    13681 gagacatcac tgaacaaacg cgattaaagg aaaaactgga gtatcaggcc actcatgacg
    13741 cattaaccaa tttacccaat cgcttgttat taattgatcg tattgaacat gctatcagtg
    13801 gttatgcccg ccataaaatg aaatttgcca tattattttt tgacttggac cgctttaaaa
    13861 tgatcaatga cagcttgagc
//
LOCUS       DASLOE010000075        13682 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.075, whole genome shotgun sequence.
ACCESSION   DASLOE010000075 DASLOE010000000
VERSION     DASLOE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13682
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.075"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            62..769
                     /locus_tag="U6309_001700"
     CDS             62..769
                     /locus_tag="U6309_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEN5505141.1"
                     /translation="MNKVITESQRFTLRNVANMLLCVDASVLPTQSNIAQKIQTKGIM
                     YNDLCKDSFDTEVPLNSNPLSIAGFTLVELLVTLSVFAIILTLIVPSLRTMILNSRLT
                     SNIDSLVSSLNYARGVALDRAVNVAVCPLGSPGSTACGANWSSGWIVVTQPVAGAPTL
                     LKSHQTSVNDPVITSNVSSVVFDPHGLSTTQSNFTMCDNRGNAFARSAMVLATGFVQS
                     GTTPGQAVWSNGALNCP"
     gene            782..1321
                     /gene="pilV"
                     /locus_tag="U6309_001701"
     CDS             782..1321
                     /gene="pilV"
                     /locus_tag="U6309_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEN5505142.1"
                     /translation="MINRMDTLAKDKGMTLLEVLISVVILAVGMLGIASMLIVSNKAN
                     NSSYAKLQAVQAVYDIFDRIRANSDAASNGSYNVSNIGSNGYPTSVSAPSVLCNASVC
                     NPNQLAQYDTWYWLTRVVSQLPNGSGSITSALSGVAGNTVITVTVQWDDSKAQNELGA
                     SSASSGTANFVQFRVQSQI"
     gene            1318..2385
                     /locus_tag="U6309_001702"
     CDS             1318..2385
                     /locus_tag="U6309_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEN5505143.1"
                     /translation="MNINYRLQQGFSLVELMIATLVGLILTYSILQIYLAQTQLYKAS
                     NSQDLIQSTENAISNLLIPVIRSAGFAGCGSIITAVSSLNAGGPRPLGILNTTPTILM
                     GYNGSGASFTITQSNPANSSNASHWSPALDSTLVGNAQQGSDVLVVLGSTSDSYPTGI
                     TTIDPGSNTFVVQGTSGMSLTSGMFGAVSDCGKTVIFQITGVAGTTITHDAGAGVLQN
                     ASSAFPVNFQTGSQFIPIQQTAFFVGQGQGGQSALMRASLVGNNWTIQPLVPGVEIMK
                     VQYGIGNNGVITRYVSANAVTDWAQVYAIRLGFLIAGQLGSGSLTTRQYNVLDTQITV
                     PADNRLRHVFELTINLRNSIS"
     gene            2382..2894
                     /locus_tag="U6309_001703"
     CDS             2382..2894
                     /locus_tag="U6309_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEN5505144.1"
                     /translation="MNVSSTNQYSKQRGYVLITSLVLMSMLTVLALTQISQNTSQTRI
                     ATNATDSEISFEKTEGAINEAINLIINGTYNPQSFLASNNGMYLFDQSATTPLWKTIN
                     WSSGSAVINSFQGNTGAQASYYLEKLPSVIMPGQSMKKPSNVYRITARSSGASGDTSV
                     LIQSTIQIQQ"
     gene            2907..6416
                     /locus_tag="U6309_001704"
     CDS             2907..6416
                     /locus_tag="U6309_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilY"
                     /protein_id="HEN5505145.1"
                     /translation="MDALTFWNFTRKGSQKAIKVLPRTLMAILLSASVSLYAVPLAIP
                     QIPLIIASPVHPQVLILLSNSQSMDGNLSGAIMTGSGSLSTGLSSLYNSSSPVNYTVP
                     AGFTPPVQGPDANGLAPYTVVQGGNKVDNSASRLNVAKAGIKAIIENYMPTTDFALGT
                     YSTSNISSYNTWVYYMSPPGSDFVFTNTPVAGNRYVTNPCYNYGSASSTVSSNCSSIG
                     SLYGTTLVSSSQYLQIGDSSDDPDINDVLYAGSGFPGIFVSYNGPTPSSPFPPNYTIS
                     NYNQGNIRISYANTRPSIGNFSSSPTNAGFVPFSQQVMYVQRGFGYYSNQSYATGNML
                     VNMQTAGTNPTTTSVNNAINAFLPHLKPETNSTATTEIKAAAVQSPLAGLLTRSRSFM
                     KTVGTTSGNCPQKQYIILISDGLPTQDLQSRYWPPLGSAAATGYGVTATFNADGSLNS
                     TNSQALSDAINEIKALKNDGVLTFIIGMGAGVDPAVNPEAAATLRAMAVAGGTENYYP
                     ATSPEALVSSLNSILSNIQNGSFSTSAAAISSTRIDSSTVEYQANFITRDTPYQDWTG
                     NLTAIELDENTGQPTNIIQWSAQSTLDSLVSGTGWSTNRKIATWDPIAADGVPFRWAN
                     INATQQAQLQPSDLLGENRLEYLRGNAALEKRNGGTFRDRSHALGDIIDSQVIYVGPP
                     SAPYLTASYISFAKANANRQPMLYVGANDGMLHAFNATTGEEFFAFIPNAVFNNLHQL
                     TSPLYNQNHLFFVNGSPESADVQFSDGSWHTILVGGENAGGKSIFALDITNPTNLSSE
                     PGVANAVLWEFTDTDLGLTYSKPQIGQIRSGSPSSLNFAVFFGNGYNNTNNKSVFYAV
                     DPQTGTLLRKIDLCAAVPSACNVNLPQGLSTVALGQKDGLQADPVTVVYAGDLQGNLW
                     AIDVSSADPASWSARVLFQARDSAGTPQPITTPPLVTLNPNYPRNPGLFVVFGTGQLL
                     TTSDLVTTQRQTIYGVWDKPLSSVPYVRANLQQQTLTLVNSATSGLSTNIITATAATI
                     NWSNKVGWFADLPIAGQRLITTPELINGAFIATINTPPLNSCGFGFTSMLLELNFLNG
                     GAFQYARFDISGDGGFDIADQYNGAYPVGIGLSNSYANSPTVLGPNKDNNLVILITQS
                     DGTQTTVIAPNITPRKIGWWQIQ"
     gene            6428..6877
                     /locus_tag="U6309_001705"
     CDS             6428..6877
                     /locus_tag="U6309_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEN5505146.1"
                     /translation="MLSHVHFMKNSRMKQSAFTLVEVLISMVIMGILVSIAYPSYLQY
                     IQKSRRADAHATLTQDQIILERCYSQNFSYAAACGALPAFPQTTPNGYYTINISNLTA
                     TTYTLTATPVGTQAKDTECASMSINQANVKTAVDSSANAQPECWNPG"
     gene            6955..9162
                     /locus_tag="U6309_001706"
     CDS             6955..9162
                     /locus_tag="U6309_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEN5505147.1"
                     /translation="MEIYCFLIGVLYAYTYNIYLTIAIPLLFYITPRYPIVLFFIAGC
                     GFALIHQWIVSPKGMPDTQVITQAQLEGTIASIPMQRPNKTQFHFAIEKINNNPAQGL
                     IQLNWYNKPPVLRAGQRWQLSVKLKKPRNFRNPGGFNYVRYLAARHIHWTGYIRYGNN
                     KLISNQPPSFSWLTLREKIGDALTKLAPNTETAGIIEALTLNVTHHISSEHWNLFRRT
                     GTVHLFGISGEHIALVSCIFFIVIRWIWSMSYRCCLFLPAHTIASIGGLLAALPYAFL
                     AGFEPPVQRALIGCVLYTIYAIGKQRFTPWQIWRYALFLVLCIEPHAVFLQGFYFSFL
                     AVACLLLTHQRWPLKNYKKTLTLQLSCLIGLMPLTLHWYSYGSINGFLANLFSIPLVS
                     FLIVPLALMTMITSPLHWSWILMKPLAWLVALLLQGLTYVEQIAGINITWSITSVTMM
                     LAMTAGLFLRVLLPVKPFKHLAILWIILPLFPPRTAIYPGEALINILDVGQGLAVAIQ
                     TQHHVLLYDTGDSYFQGSDLGQMAIIPFFRSIGIKTIDKIVISHPDKDHKGGLNSLEK
                     EMQVNQLIVNDPYFYKRGSNCHSYPQWQWDGVSFRFLPIKESFTKKNNNSCILQVSNS
                     AGRVLLTGDIEKAAEEYLVRNYGSQLASEILVVPHHGSKTSSSYRFLLEVNPLYAIAS
                     LGFDNRFKFPHSKTLQSMKTLDITFFRTDECGMAAIKLPASGQVHRPSCMQAN"
     gene            complement(9218..10525)
                     /locus_tag="U6309_001707"
     CDS             complement(9218..10525)
                     /locus_tag="U6309_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN5505148.1"
                     /translation="MHTQRGLEVTTGSIEAVDSINYFHEQVLSSGQNAIQILDSAKKH
                     TDNLLIQTYAAAFYLYAQEDVANEQASDYLQSASRLLTSANEREKLTFEAVTNWSKRD
                     YANAISLLVTLLERYPRDTLALKFVEWLFYCTGQAFNAEYFLKVCDKCAPENQDESHF
                     LAIHSFALELCGQYSKAREMAEEAITMNLHTPWAHHTLAHVHLLTSDITGGINRLRDL
                     QKTWEDILPLLKGHNTWHLALFYLANRDEEEVKKLYPHISGALPDTVLEQLDTISLLW
                     RMDMAGLPQDRLLNQVVDHLSTHPLEFYTGFTNAHFIYCLVKSGYKNEADESLKRMKS
                     YACAPSSDALWGDVVLPLCQGIYAFADADYKTALMLMEPVIGECAQLGGSDAQIELFF
                     QTYLLVLIHTNQKDKALQFFTEHLKYYNNTPLSDWWFQSANKT"
     gene            complement(10726..11103)
                     /locus_tag="U6309_001708"
     CDS             complement(10726..11103)
                     /locus_tag="U6309_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505149.1"
                     /translation="MMKKINLFKLLMIFVLGTSALQAAGLSTQRIEQLSNARVTVWKT
                     IIYPSSKQVLPMHRHDNDRVLVALTDGTLKITNNKGKVHYLKLKKGKSYYLTKDVPNE
                     LHNDENVTNHSIQVMVIQLNDKA"
     gene            11240..11632
                     /locus_tag="U6309_001709"
     CDS             11240..11632
                     /locus_tag="U6309_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505150.1"
                     /translation="MLTTFLATVIGWYLVIVSLFLLFHNEHAKSAMAEIIAHRGSQLI
                     MAIVTLITGLLLVVSHNIWIMGWPVVITLFAWLVLLGGLIRFLCPDTAVRVGQSFVDN
                     PMRLKIAAVIFFIIGLFLLFNVYYVYLK"
     gene            11758..>13682
                     /locus_tag="U6309_001710"
     CDS             11758..>13682
                     /locus_tag="U6309_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505151.1"
                     /translation="MTRAQTHPHQTTVISNLKIIPLTLGWLLKFALLIIIIAVFTPLS
                     PKMPAPGLDASWAVGLNQAVAQGLAFGKDIFFTLGPYSSIYTKFYHPATNFMMVGGSL
                     YLALCYWIAIILLMQGIKWRWSIAFAILLFSMIYARDSLFFSYSLIIGLLIHKEIPGK
                     ETSLKSNFFTLFALLFTPFGLFPLIKGSLLILSIITILLCSFFLSLYKQRNLVVLCLA
                     SPVVSILFFWIASGQSVFNLPAYISNTISLASGFTEAMATEGDNKELIFFGITSSCIL
                     LSILLQKQLSPLSKAFLFCIYFVFLFLSYKTGFTRHFGHAFIAGTSVLLASFLLPYFN
                     SNNKVIVPLLALSLYTANYINGHYTKISVRDNFISTYTAAYYGLQNRIQNKNWLKENF
                     SLTMNYLKARDDFPRLPGTTDIYSYNQTSLIASGMQWSPRPIFQSYSVFTAKMAEINT
                     NYLLSENKPDNIIFKVEPIDNRIPSMEDGMSWPTLINHYQPVRLENDFLFLRKKLVNK
                     PVTSLVPLKTEIHTFGEQVKIPDTDKPLFVEIEIQPTLWGRLATTFFKPHQLEANLEL
                     KNGTQRQYRLISNMAKSGFLLSPLIENSAEFGLLFSQNNYLAGKKVISFSINSKQVPS
                     SHWQTKYTIHYKIFASN"
BASE COUNT         4005 a         2990 c         2633 g         4054 t
ORIGIN      
        1 taactaattt gaactataat taataatgta gtgggttcga tcaaacgata gggataatgc
       61 tttgaacaaa gtgataacgg aatcacaaag gttcactctg cgcaacgtag ctaatatgtt
      121 attgtgtgtg gatgctagtg tattgcccac acaatctaat atagcacaaa agatacaaac
      181 aaaaggcata atgtacaatg acctttgtaa agacagcttc gatactgagg tccctcttaa
      241 cagcaaccct ctttccatag ccggattcac actggttgaa ttattagtaa ccctatctgt
      301 ttttgccatt atcttaactc taatcgtccc ttcattaaga actatgattt tgaatagccg
      361 tttgacttca aacatcgact cactggtaag ctcattaaac tatgcgcgtg gagtggcact
      421 tgatcgggca gtcaatgtgg cagtatgtcc attaggttca ccaggttcaa cagcctgtgg
      481 agccaactgg agctcaggat ggatagtagt gacccaacca gtagcaggag caccaacgct
      541 tttaaaatcg caccagactt cagtaaatga tcctgttatc acttcgaacg tatccagtgt
      601 tgtttttgac ccacatgggt tatcaacaac ccaaagtaac ttcactatgt gtgataaccg
      661 aggcaatgcg tttgcccgct cggcaatggt tttggccact ggatttgtcc aatcaggaac
      721 aacacccggc caggcagtct ggagtaatgg agctctgaac tgcccttaac ggagatagaa
      781 tatgattaac cgaatggata cattagcaaa agacaaggga atgactttgc tggaagtgtt
      841 gatatctgta gtcatattag ccgttggcat gctagggatc gccagtatgt tgatagtcag
      901 taataaagcg aacaattcca gttatgcaaa actgcaagca gtacaagcag tctatgatat
      961 ttttgacaga attcgagcga attcggatgc agcatctaat gggagttata atgtcagtaa
     1021 tatcggcagt aatgggtacc ctacgtccgt aagtgctcct tcagtattat gcaatgcatc
     1081 ggtatgcaat ccaaatcaac tggcacaata tgacacctgg tattggctca ccagagtggt
     1141 gtcgcaattg ccaaatggaa gcggttcaat aacttctgct ctttcaggtg tggcaggaaa
     1201 taccgtgatt accgtgactg tacaatggga tgatagtaaa gcacaaaatg agttaggggc
     1261 aagcagcgct tcttcaggaa ctgccaattt cgttcaattc agagtacaga gtcagatatg
     1321 aatattaact acagactaca acaaggattt tcattagtcg agttaatgat agccaccttg
     1381 gttggactga tactcactta ttcaatttta caaatatacc tggctcaaac ccaactttac
     1441 aaagcatcaa actctcagga tcttattcaa agtactgaaa atgccatatc caatttactc
     1501 ataccagtga tcagatcggc tggatttgcc ggctgcggat ccattatcac cgcagtttcc
     1561 agtctaaatg ctggtggccc aaggcctttg ggaatattga atacaacgcc aacaatcctt
     1621 atgggttaca atggcagcgg agcgagcttc acaataaccc aaagcaatcc tgcaaatagc
     1681 agcaatgcca gtcattggtc acccgcatta gattccaccc ttgttggcaa tgctcaacaa
     1741 ggtagtgatg tgttggtagt attaggctct acctctgaca gctatccaac aggaataacc
     1801 acgattgatc ccggcagcaa tacctttgta gttcaaggca ctagcgggat gtcccttacc
     1861 tctggaatgt ttggagcggt ctctgattgt ggtaaaaccg taatatttca aattactgga
     1921 gtagcaggaa caacgattac tcatgatgca ggcgctggag tgctacaaaa tgcaagcagt
     1981 gccttccctg tcaattttca aaccggctcc caattcattc ccatccaaca aactgcattt
     2041 tttgtgggtc aaggtcaagg agggcaaagc gcattaatgc gggcctctct cgttggcaac
     2101 aactggacaa tacagccatt agttcctggg gtggaaataa tgaaagtcca gtatggtatt
     2161 ggcaataatg gcgtcattac tcgatatgtc tctgctaatg cagtaactga ttgggcacag
     2221 gtttatgcca ttcgtttggg atttttgata gcaggtcaac tcggctcagg aagcttgaca
     2281 accagacaat acaacgtttt ggatacgcaa atcaccgtac ctgcggataa ccgcttaagg
     2341 catgtatttg aattgaccat caacttgagg aactcaatat catgaacgta tcctccacaa
     2401 atcaatattc aaaacaacgg ggttatgtcc tgataacttc tcttgtactt atgtcaatgc
     2461 ttaccgtttt ggccctgacc cagatatcac aaaacacgtc tcaaaccagg attgcaacta
     2521 acgcaaccga ttcggaaatc agttttgaaa aaactgaagg tgccattaat gaagccatta
     2581 atctgatcat aaatggtact tataatccac aaagttttct tgcttctaat aatggaatgt
     2641 atttatttga tcaaagtgct actactccac tttggaaaac gattaactgg tcaagtggtt
     2701 cggcagtcat taacagcttt caaggcaata caggggcaca agccagctat taccttgaaa
     2761 aactgccgtc cgtgattatg ccaggccaga gtatgaaaaa gccaagtaat gtctacagaa
     2821 taactgctcg gtcatcagga gcaagcgggg atacttccgt acttatacaa agtacaattc
     2881 aaattcaaca gtaggttttg attatcatgg acgctttaac attttggaat ttcaccagaa
     2941 agggaagtca gaaagcaata aaagttctac ctcgaacact tatggctatt ctgttgtctg
     3001 cttccgtttc tctttatgcc gttcctttag caattccgca aatcccacta atcattgcca
     3061 gccccgttca tcctcaagtg ttgattctcc tcagtaactc ccaatcgatg gatggtaatt
     3121 tgagtggagc aatcatgaca ggatccggct ctctgtcaac tggtttaagc tccctttaca
     3181 attccagctc accagtgaat tataccgtac cggctggatt tactccgcca gtgcaaggcc
     3241 ccgatgccaa cggcctggct ccttatactg tagttcaggg cgggaataaa gtagataaca
     3301 gcgcaagccg tctcaatgtg gccaaagctg gaatcaaagc gattattgaa aattacatgc
     3361 ctaccaccga ctttgcattg ggcacttaca gtacaagcaa catcagttct tataacactt
     3421 gggtttatta catgtcgcca cccggcagtg actttgtgtt caccaacacc ccggtagctg
     3481 gaaatcgata cgttaccaac ccttgttata attatggttc cgcatcgtct acagtttcaa
     3541 gcaactgctc atccattgga agcttatatg gtacgacttt ggtttcttca agccagtatc
     3601 tgcaaattgg tgattccagc gatgatccgg atattaatga tgtactctac gctggaagcg
     3661 ggtttcccgg gatttttgtc agttataatg ggccgacacc atccagtcct tttcccccca
     3721 attatacgat ctccaattat aaccagggca acatcagaat ttcctatgcg aacacaagac
     3781 cttctattgg caatttttcc tcttctccca ctaacgccgg ttttgtacct ttttcacagc
     3841 aggtaatgta tgtgcaaagg ggatttggat attacagcaa ccaatcctat gccactggca
     3901 atatgctggt gaacatgcaa acagcgggta ccaatccaac gactaccagt gtcaacaatg
     3961 ccattaatgc ctttttacct catttgaaac cggaaaccaa ctccactgcg accactgaaa
     4021 taaaagcagc tgcagtacaa tcccccctcg ccggactact gacccgctca cgaagtttca
     4081 tgaaaactgt gggtacaacc agtggcaatt gtccgcaaaa acaatacatc atattaattt
     4141 ctgatggatt gccaacccag gacctccagt caaggtattg gccaccacta ggcagcgcgg
     4201 ctgccaccgg ctatggggtc accgcaacat ttaatgccga tggctcactg aacagcacga
     4261 acagtcaggc cctgtccgat gcaataaacg aaataaaagc tttaaaaaat gacggtgttt
     4321 taacttttat aatagggatg ggagcaggtg tggatcctgc tgtaaatcct gaggcagcag
     4381 caactctcag ggccatggcc gttgcaggag gcactgaaaa ctattatccg gcaacgagtc
     4441 ctgaggctct ggtcagcagt ttaaattcca ttctatcgaa tatccaaaat ggctcttttt
     4501 caacctctgc agcagccatt agctcaactc gtatcgacag cagcaccgtg gaatatcaag
     4561 ccaactttat tacaagagat acaccttatc aggattggac tggtaatttg acagctattg
     4621 aactggatga gaatactggc caaccaacaa atattataca atggtcagct caatcgacac
     4681 ttgattccct ggtatcagga accggatggt caactaaccg aaagatagcg acttgggatc
     4741 ctatagccgc agacggagta ccgtttcgct gggcgaatat taatgccacc caacaagcac
     4801 aactgcaacc aagtgatttg ttaggagaaa accgattaga atacctgcgt ggcaatgccg
     4861 ccctggaaaa aagaaacggg ggaacattcc gtgatcgctc gcatgctttg ggagatatta
     4921 tcgatagcca ggtaatttat gttggtcccc caagtgcccc atatttaacc gccagttaca
     4981 tcagctttgc aaaagccaat gccaatcgac agccgatgtt atacgttggg gcaaatgacg
     5041 gaatgcttca cgcctttaat gcaaccactg gagaagagtt ttttgcattt attcccaatg
     5101 ccgtgtttaa taatctccac caactcactt ctcctctgta caaccaaaat catttattct
     5161 tcgtcaacgg ctctccggaa agcgctgatg tacaattttc cgatggttcc tggcatacca
     5221 ttctggtggg aggtgaaaat gcaggtggta aaagcatctt tgccctggac attacaaatc
     5281 cgacaaacct tagttctgaa ccaggagtgg ccaatgctgt gctctgggaa tttacagata
     5341 ctgacttagg gttaacttat agcaaacctc aaataggtca aatcagatcc ggaagtccat
     5401 cttcattaaa ttttgccgtc ttttttggca atggatacaa caatactaac aacaaatcgg
     5461 tattttatgc cgtagaccct caaacaggaa cactactaag aaaaatagat ctttgtgctg
     5521 ctgtcccttc agcctgcaat gtcaatttac cccagggatt atcgacagtc gctttgggac
     5581 aaaaagacgg attgcaagcc gatcctgtta ctgtagtgta tgccggtgat ttacaaggca
     5641 atctatgggc aatcgatgtt tcctcggctg atcctgccag ttggagtgcg cgtgtattat
     5701 tccaggcaag agactctgcg ggtacaccgc agcctatcac tactcctcct ttagtgacat
     5761 taaatcctaa ttatcctcgc aatccaggac tttttgttgt gtttggcacc ggacaacttc
     5821 tgaccacatc cgatttggtc actacccaaa ggcaaaccat ttatggagta tgggacaagc
     5881 cgctttcatc cgtaccttat gtacgtgcca atctccagca acaaacgttg accttggtta
     5941 actcagctac atcaggttta tccacaaaca ttatcactgc cactgctgct accatcaatt
     6001 ggagtaacaa agtgggatgg tttgccgatt tgcctatagc gggacaaaga ctcattacta
     6061 cacctgaact aattaacggg gcatttattg caacaataaa taccccacct cttaattcgt
     6121 gcggctttgg attcacatcc atgcttctgg agcttaattt cttaaatggc ggggctttcc
     6181 aatatgcgcg atttgatatt tccggagatg gcggctttga tatagccgat cagtataatg
     6241 gggcctaccc agttggaatt ggcttgtcca acagttatgc taactcacca actgttctgg
     6301 ggcctaacaa ggataacaat ctggtcattc tgatcacaca atcggatgga acacaaacca
     6361 ctgttattgc acctaacatt acgccaagaa aaataggctg gtggcaaatt caatagagga
     6421 tacccaaatg ctcagtcatg tacattttat gaagaatagc cgtatgaaac aatccgcgtt
     6481 taccctggtt gaagttctga tcagcatggt cattatggga attctggttt caattgccta
     6541 tccatcctat ttacaatata tacaaaaatc ccgtcgtgct gatgctcatg ccacattgac
     6601 acaagatcaa atcattttag aacgctgtta ttcacagaat ttttcctatg cggcagcgtg
     6661 tggcgcccta ccagcatttc ctcaaacaac gccgaatggg tattatacta tcaatatttc
     6721 aaacctgaca gccacaacgt ataccttaac cgcaacccct gtcggaaccc aagccaaaga
     6781 taccgagtgt gccagtatgt caattaacca ggccaatgta aaaacagccg tagattcctc
     6841 cgctaatgcg caaccagaat gctggaatcc cggttaatac tgtaaaaaat tctttacaca
     6901 aaggattcaa ttcgagtaga tttgccagta agggaaagtc caacttaatt tgaaatggaa
     6961 atttattgct ttttaatagg cgtactttat gcctatacct ataatattta tctaactatt
     7021 gcgattcctt tactctttta tatcacacca agatatccaa ttgtcctgtt cttcatcgcc
     7081 gggtgcggct ttgccctgat tcatcagtgg attgtatctc ccaagggcat gcctgatacg
     7141 caagtaatca ctcaagccca attggaagga acaattgctt ccatccccat gcaacgtcct
     7201 aacaaaactc aattccattt tgcaattgaa aaaattaata ataaccctgc tcaaggcttg
     7261 atccaattaa actggtataa caaacctcct gtcttacgag ccggtcagcg atggcaactt
     7321 tcagtgaaac taaaaaaacc tcgtaatttc cgcaacccag gaggttttaa ttatgtacgt
     7381 tacctggcgg ctcgtcatat ccattggact ggttatattc gttatgggaa taataagcta
     7441 attagcaatc aacccccttc tttcagttgg ttaacactgc gtgaaaaaat aggggatgct
     7501 ttgacaaaac tggcaccgaa tacagaaacg gcaggcatca tcgaagcatt gacgttaaat
     7561 gttactcacc atatcagttc agaacattgg aatttgttcc gaagaacagg aacggtacat
     7621 ttattcggga tttcagggga gcacatcgct ctggtctcct gcattttctt tatagtgata
     7681 agatggattt ggtcaatgag ctatcgttgc tgtttgttct taccggccca taccatagcg
     7741 agtatcggtg ggctactggc cgccttgccc tatgcttttt tagccgggtt tgaacctcct
     7801 gtacagcgtg ctttaattgg ttgtgtactt tacacaatat acgccatagg aaaacaacgt
     7861 tttacaccat ggcaaatatg gagatacgcc ttatttttag tcttgtgtat tgaaccccat
     7921 gccgtattcc tgcaaggttt ttatttttca tttcttgctg tggcctgtct tctattgacg
     7981 caccaacgtt ggccactgaa aaattataaa aaaacactga cattacaatt aagctgcctc
     8041 attggattga tgcccttgac tctccattgg tactcctatg gttctattaa tggattcctt
     8101 gccaatttat tctccatacc tttagtcagc tttctgatag tacccttggc tttaatgact
     8161 atgataacga gtccgcttca ttggtcctgg atattgatga aaccattggc atggcttgtt
     8221 gccctgttac tgcaaggact aacgtatgtt gagcaaatag cagggattaa tatcacctgg
     8281 tccatcacct cggtaacaat gatgctcgct atgacagcag gtctgttttt aagggtatta
     8341 ttacctgtca agccattcaa acacttggct atcctttgga ttatccttcc cttatttccg
     8401 cctcgtactg caatttatcc aggggaagca ttaattaata ttttggatgt tgggcaagga
     8461 ctggctgttg cgatacaaac gcaacatcat gttttactct acgatacggg agattcctat
     8521 tttcagggga gtgatttagg gcaaatggcg ataataccct ttttcaggtc aattggaata
     8581 aaaacaattg acaaaatagt aataagccat cctgataaag atcataaggg cggattaaat
     8641 tcccttgaaa aagagatgca agtcaatcaa ttaatagtga atgatcccta tttttataaa
     8701 cgtggaagta attgtcattc atacccacaa tggcaatggg atggggtgag ttttcgtttt
     8761 ttacccatca aggaatcttt cactaaaaaa aataacaatt cttgtatctt acaggtaagt
     8821 aattcagctg gcagggtttt attaacggga gatattgaaa aagcggctga agagtacctg
     8881 gtaagaaact acggcagtca gctggcatcc gagattttag ttgtgcctca tcatgggagt
     8941 aaaacgtctt catcctatcg atttttgctg gaagtaaacc cgctttatgc cattgcatcc
     9001 ttaggatttg ataatcgttt caaatttcca cattctaaaa ctttgcaaag catgaaaaca
     9061 ttagatatca ctttttttag aacagacgaa tgcggaatgg cagcaatcaa actacctgca
     9121 tcaggtcaag ttcacagacc aagttgtatg caggctaatt gaatagatgc attggctggc
     9181 caatgtattt tacttcatca ttccaagtgc tatacctcta agtcttatta gccgattgga
     9241 accaccagtc actcaatggt gtattattgt aatactttaa atgttcagta aaaaattgta
     9301 acgctttgtc cttttgatta gtgtgtatca acaccaacaa gtaagtctgg aaaaaaagct
     9361 ctatctgtgc atcgcttcca cctaattgtg cacactcacc aatcaccggc tccatcaaca
     9421 tcaaggcagt tttgtaatct gcatcagcaa aggcatagat accctggcac aaaggtaaaa
     9481 caacatcgcc ccatagagca tcagaagaag gcgcacaggc gtaagacttc attcttttca
     9541 acgactcatc cgcttcgttt ttataacccg atttcaccag acaataaata aaatgagcgt
     9601 tcgtaaaccc ggtataaaat tccaaaggat gggtacttaa atggtccaca acctgattta
     9661 acagcctgtc ttggggtaga cctgccatat ccatgcgcca taacagggaa atagtatcca
     9721 actgctcaag caccgtatca ggcaatgccc ctgaaatatg ggggtataac tttttaacct
     9781 cctcttcatc acgatttgcc aaataaaata gcgctaaatg ccaggtgtta tggcctttca
     9841 acaaaggtaa aatgtcctcc caggtttttt gtaaatctcg aagtctattg ataccaccag
     9901 tgatatcact tgttaataaa tgcacatgag ccagggtatg atgtgcccaa ggggtatgca
     9961 aattcattgt gatagcttcc tcagccattt ccctggcttt tgaatattgc ccacacaact
    10021 ccaaagcaaa ggaatgtatg gctaaaaaat gggactcatc ctgattctcg ggagcgcact
    10081 tatcacatac tttaagaaaa tactcagcat taaaagcctg tcctgtacag taaaaaagcc
    10141 attccacgaa tttaagagcc agggtatccc tgggatacct ttcaagcaga gtaactaaca
    10201 agctaatggc attggcataa tctcgcttag accaattggt tactgcttca aaagttaatt
    10261 tttcacgttc attcgctgat gttaataacc tggatgctga ttgcaaataa tcgctggctt
    10321 gttcattcgc tacatcttct tgtgcataca aataaaaagc agccgcataa gtttgaatta
    10381 ataaattgtc agtatgtttt tttgctgaat ccagaatttg gattgcgttt tggccggagc
    10441 ttaatacctg ttcgtgaaaa taattgatac tatctactgc ctctatggag cctgtcgtta
    10501 cttccaatcc acgttgagta tgcattctcg aaatcctgtc aatgaatgaa tcaatgatta
    10561 aatgatagca caaataataa acaaaaagga cggaagtaac gctccaatta gataataata
    10621 cgacctgata tcacgatgac tgcggtagag ctacaagcac tcttgctaaa attgtttttc
    10681 ctggccagga attaatacaa catcagatta cccacttccc gctaatcatg ctttatcatt
    10741 gagttgaatt accatcacct gaattgaatg gttagttacg ttttcatcgt tatgtaattc
    10801 gttaggaacg tctttggtaa gataatacga cttgcctttc tttaatttta aatagtgtac
    10861 tttccctttg ttgtttgtaa ttttcaatgt cccatccgtc aatgcgacca aaactcgatc
    10921 gttatcatgc ctgtgcatgg gcaaaacttg ttttgaagag ggatagataa ttgttttcca
    10981 cacggttacc ctggcattag ataattgttc aatacgttgg gttgataatc ctgcagcctg
    11041 aagtgcactt gtacccagaa caaagatcat aagcaatttg aataaattaa tttttttcat
    11101 catgttagat tattctccat tttcaaatca aataacagtg tgtcatttcg cttattcctc
    11161 aattttcctt taactttccg gcacatagct cacaaaattg ctataattat agtaggaggt
    11221 aattgtaagg agtttgaaga tgttaactac atttctagcc acagtgattg gctggtattt
    11281 agtcatagta agcttattct tattattcca taatgagcat gcaaaatcag ctatggcaga
    11341 gattatagct catcgcggat cgcaattgat tatggctata gtaaccttga ttacaggttt
    11401 gcttttagtc gtcagtcata atatctggat aatgggctgg cctgttgtta ttactctttt
    11461 tgcatggtta gtcttgcttg gtggtttaat aagattttta tgccccgata ccgcagttag
    11521 agtaggccaa tcatttgtcg ataatccaat gagactaaaa atagcagctg taatattctt
    11581 tattattggt ttgttcctgt tatttaatgt ctattatgtt tatttgaaat aaaggaagag
    11641 ttattggggt actgtgactc attctgttta tggggaaaag gaagcgataa agtccttgtc
    11701 aattcagcag caaggaatgc tatgatatga tgcttgatat gatataccta caaaaaaatg
    11761 actagagccc aaacccaccc tcatcaaacg accgttattt caaacctcaa aataattcca
    11821 ctcactttgg ggtggttatt aaaatttgct ttgctcatta ttataatcgc tgtttttact
    11881 ccactatccc ccaaaatgcc agctcccggt cttgatgctt cttgggctgt aggacttaat
    11941 caagccgttg cccaaggttt ggcctttggt aaagacattt tttttacctt agggccttat
    12001 tcttccatct atacaaaatt ctaccaccct gctaccaatt ttatgatggt tgggggaagt
    12061 ttgtatttgg ctttatgtta ctggatagct attattcttt taatgcaagg cattaaatgg
    12121 cgttggagta ttgcttttgc aatacttctc tttagtatga tttatgctcg ggattctttg
    12181 tttttttcct attcattaat cattggccta ctcattcata aagaaattcc tggaaaagaa
    12241 acatccttga aaagcaattt cttcacttta tttgctttgc tttttacgcc attcggttta
    12301 tttcccctga tcaagggctc tcttttaata ttaagtatta ttaccatact cttatgctct
    12361 ttctttttga gcctatataa acagcgtaat cttgttgtgc tttgtctggc atctccagta
    12421 gtttccatat tgtttttttg gatagcatcc gggcaatctg ttttcaatct gccagcttat
    12481 atcagtaaca ctatctcgct cgcatcaggt tttactgaag ccatggcaac tgaaggagac
    12541 aataaagaac taatattttt tgggattacc tcttcttgca ttctcttatc aatactcctg
    12601 caaaaacagc tctcaccatt atccaaagca tttctatttt gcatctattt tgtttttctt
    12661 ttcctctctt ataaaacggg gttcacaaga cattttggcc atgccttcat tgctggtacc
    12721 tctgttttgc tagcctcttt tttattgccg tattttaatt ccaacaacaa agtgatagtc
    12781 ccattattag ctctatccct ttatacagcg aattatatca acgggcacta taccaaaata
    12841 tctgttcgag ataatttcat ttccacctat accgccgctt actacggcct tcaaaacaga
    12901 atccagaata agaattggtt aaaagaaaat ttttcattaa cgatgaatta tttgaaagcc
    12961 cgtgatgatt tccccagatt accaggtact accgatattt attcctacaa tcaaacttca
    13021 ttaatcgcct cgggaatgca atggtcgcca aggccaatct ttcagagtta ttccgttttt
    13081 actgcaaaaa tggcagaaat caatacgaat taccttttaa gcgagaacaa accggataac
    13141 atcattttta aagtagaacc aattgacaat agaattcctt caatggaaga cgggatgagt
    13201 tggccgacac tgattaatca ttatcaacca gtacggttag aaaatgattt tttattttta
    13261 agaaaaaaat tagttaacaa acctgttaca tctttagtgc cattaaaaac agaaattcat
    13321 acttttggag aacaagttaa aatacctgat accgataaac ctctttttgt cgaaatagaa
    13381 attcaaccaa cgctctgggg gaggttagcg actacatttt ttaagcctca ccagcttgaa
    13441 gccaatttgg agttaaaaaa tggtacccaa agacaataca ggctcatttc caatatggca
    13501 aaatccggtt ttctattatc gcctttaata gaaaacagcg ctgaatttgg attgttattt
    13561 agccagaata attacctggc aggtaaaaaa gttatttctt tttccattaa ctcaaagcaa
    13621 gtgccttcct cgcactggca aacgaaatat actattcatt ataaaatttt tgccagcaat
    13681 aa
//
LOCUS       DASLOE010000076        13674 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.076, whole genome shotgun sequence.
ACCESSION   DASLOE010000076 DASLOE010000000
VERSION     DASLOE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13674
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.076"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            100..360
                     /locus_tag="U6309_001711"
     CDS             100..360
                     /locus_tag="U6309_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505152.1"
                     /translation="MKILVNSSVLAIVLALSTPLFADDTQWMTEHNLVKGVITFQDDS
                     QIQVYAAQADLDKLQQNLANIQSVEVQPNQTDSTTSPNQQSE"
     gene            complement(403..672)
                     /locus_tag="U6309_001712"
     CDS             complement(403..672)
                     /locus_tag="U6309_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEN5505153.1"
                     /translation="MSLGELLLIFIVAIIVFGPSKLPMLATHLGMLVRKINQLKEHTS
                     SFWQQQLNEIQLYENQRKAEKADQQYQQTDSENQNSDHKKNTRPS"
     gene            complement(669..854)
                     /gene="tatA"
                     /locus_tag="U6309_001713"
     CDS             complement(669..854)
                     /gene="tatA"
                     /locus_tag="U6309_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEN5505154.1"
                     /translation="MGLSGISPLSLLLILAIIVALFGTSKLKTIGSDLGEAIKNFRKA
                     MNSEETNDTQKDDHKPS"
     gene            complement(856..2505)
                     /gene="ubiB"
                     /locus_tag="U6309_001714"
     CDS             complement(856..2505)
                     /gene="ubiB"
                     /locus_tag="U6309_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEN5505155.1"
                     /translation="MKSIKQLIRLIHINYILAKNGLDNVVVSIKLFAPLRFIVYLNPW
                     NWLRKEKLTRGEALRKSLEELGPIFIKFGQALSTRPDILPEDIAKELSKLQDKVPPFP
                     SHVAMNIIEQAYKQSAYDVFAQFDPIALASASMAQVHAATLKTGENVVVKILRPNMRR
                     LIEQDLSIMYTIATLADRYWPEGKRFKPKEIVKEFEHTLLDELDLMREAANAAQLRRN
                     FNQSPMLYIPEIYWDYCHHNILVIERIHGIPVTDIASLRDHGIDIKKLAERGVEIFFT
                     QVFRDCFFHADMHPGNIFVSYQNPKDPQYICVDFGIIGTLTDNDKRYLAENLVAFFNR
                     DYKRVAELHVESGWVSRDTPVAEFESSIRTVCEPIFEKPLKDISFGQVVFRLFQVARR
                     FHMEVQPQLILLQKTLLAIEGLGRQLYPELDLWATAKPFLEKWVREQMGPKAFIKRLK
                     QNLPFFTEQLPHMPKLIFDILEMKKDQLLTVHELTRLQSENNNQTIQWKSVGLGVFFS
                     FLSLGAISYFDLLDYNHLTTITIAGSVLAGIFVLINRKIRN"
     gene            complement(2595..3212)
                     /locus_tag="U6309_001715"
     CDS             complement(2595..3212)
                     /locus_tag="U6309_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEN5505156.1"
                     /translation="MLKEYSLKALQTAINQAMKLDEQMPQKLQKLDGKTLEMVITPLN
                     VNFYIRFKGSEMQLLHRIDGRPDTIIHSNPIGLIRLSLLPTSKARSLFNDKIRISGDI
                     ELGQSVKKLFDEIDIDWEGHLAHFTGDVVAHQIGSFVRKGLEFKNQFSTSMQQNITEF
                     LQEELRISPSRNELEDFFAEVDELVLSVDRLQAHINNLMSDDEGN"
     gene            complement(3220..3972)
                     /gene="ubiE"
                     /locus_tag="U6309_001716"
     CDS             complement(3220..3972)
                     /gene="ubiE"
                     /locus_tag="U6309_001716"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEN5505157.1"
                     /translation="MTNQKQTTHFGFKSVDWNEKEKKVAEVFHSVAKNYDRMNDLMSL
                     GIHHLWKRYTIELSHVRPGQSVLDLAGGSGDLTRLLLQKVGDSGQVILADINAAMLHV
                     GRDRLLDEGLFKNIRYVQGNAQCLPFADNSFHCITMGFGLRNVTDKDEALQSMYRVCK
                     PGGKLMVLEFSTPVFPGLKPVYDWYSFNILPKIGKFVANDEASYQYLAESIRMHPDQE
                     TLKAMIERVGFEDCHYHNLSGGIVALHIAYKY"
     gene            complement(4097..4522)
                     /locus_tag="U6309_001717"
     CDS             complement(4097..4522)
                     /locus_tag="U6309_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEN5505158.1"
                     /translation="MFYGDTVQETRQLFFISWNKYQNKKELSPLEKEIAQVILDHPEY
                     HKVIEQIPSFPEHTYYPELGETNPFLHMGLHLAVREQVATDRPQGIRTVYTKLIEKYQ
                     DPMAVEHLIMDQLAECLWLSQKNNRPPDENHYLITLQNL"
     gene            complement(4748..5353)
                     /locus_tag="U6309_001718"
     CDS             complement(4748..5353)
                     /locus_tag="U6309_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HEN5505159.1"
                     /translation="MLIYFNGLLLGLSLIMALGPQNVFLIRQGARKNHAALSAVICFF
                     CDVILVCGSVAGLHQLLLAHPNLQIWMVWLGSAFLIYYAIKNLKSALSKNKQTVVETH
                     QPHTRMQIILFALGFSLLNPHAIIDTLVIVGSGSSQYPDHKLAFLAGVISASMIWFSS
                     LTMTTRYFSNLLTQNKVWQKIELLSGILMAYIAMKLITSSL"
     gene            complement(5411..6439)
                     /locus_tag="U6309_001719"
     CDS             complement(5411..6439)
                     /locus_tag="U6309_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN5505160.1"
                     /translation="MKIFNAMFSKVNGGLEQVFLNYIPALSSQGNLVIPIIHPKAEIR
                     NSCPQDHLITVHNFNQHDFFAIHRLKKLIKIHQPDCIITHSYRAAYLFKKTRTKVPKI
                     AVCHVKGHYDFGTDAIIALTEQMRRDIINSGIEERRVFTIPNLIHIPDGLQYREPKNN
                     QIPIIGVCARFAAIKGVDVFINALAELKKRGVAFQAKIAGDGKEKEQYIELIRHHGLE
                     HEITLLGWIEDKESFYKSIDIFCLPSREESFGLVVLESMMHSLPMVLTRLSGPLEIAG
                     NSGCALFVPPEDPVSMADGLERLLRDKDLSKQLALKAFQRVQHYSSTKVAPMLHKVLE
                     KACEINQQ"
     gene            complement(6502..6855)
                     /locus_tag="U6309_001720"
     CDS             complement(6502..6855)
                     /locus_tag="U6309_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEN5505161.1"
                     /translation="MSFKPAVELVKLQQNNREVAVIDGQKILIIWHDNQVHAIQSQCP
                     HLKLPLTKGKITESCSIICPFHKSEFDLKTGEAKCWSPWPPVVGNLLGKVSSRKHLKI
                     YPTQIENGQVLVDVG"
     gene            7237..8850
                     /locus_tag="U6309_001721"
     CDS             7237..8850
                     /locus_tag="U6309_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEN5505162.1"
                     /translation="MGSLVIKKSIIAALVVALGIAGQSVQAESVPDETLQSEKVQTEK
                     VQAEKTETDLFSPVEQIMNPFYPKYPPTKPATGEQAELIKRGEYLAKMGDCIACHTDV
                     EGGTPAYAGGLPINTPFGLFYSPNITPDKETGIGNWTEKDFIRALKDGRDPKGRNYFP
                     VFPYVYFSKITDDDARALYAYFMSIPPVKLKNKSLPFPFGVPGARFALWGWNLLFFFP
                     QENSIEYDPDKSPEWNRGRYIVDTLGHCSMCHTPMNIFGSAKNRYYLTGSFIDGYWAP
                     NITKYGLESASHQEVADVFKAGELINKAGPVAGPMAEVNHNSLSHLTEEDRLAIATYL
                     KTVESEEYLGVSPSDLPPSVARGRQVYLKACLICHKDNKMGAPVIGDSASWYTRLKNS
                     GLTGLYRHAIYGYNQMPVKGACVTCSDNDIISAVDYLLNASLTRSQWQDLASGGVKKY
                     PASGKAVYNENCAVCHNEGKLGAPKIGDKVTWKPLIAQNMDVLIENTIRGKNHLPNGG
                     CNHCSTDEVIEAIKYMVSQSKTEGNYSLW"
     gene            8932..10137
                     /gene="coxB"
                     /locus_tag="U6309_001722"
     CDS             8932..10137
                     /gene="coxB"
                     /locus_tag="U6309_001722"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEN5505163.1"
                     /translation="MLNRLNICKLIALFSALTSAQSLWAAADEWQLNMYKGVTPLSND
                     MYYLHMVAMAVCAVIGVVVFGVMIYSLIHHRKSKGYQPASFHDNPKLEIIWTIIPFLI
                     LVGLAVPATRVLIRMDDTQLSDVTVKVVGYQWKWQYQYLDQGISYFSSLSTPYEQIEN
                     KQKKNQWYLLEVDNPLVVPVNKKIRFLVTSNDVVHSWWVPELGVKRDAIPGFMHEAWA
                     RIEKPGVYRGQCAELCGINHGFMPIVVKAVSEEEFDRWVSEQTKVEDIYAEKSDKPRE
                     QKTMTRDQLMTLGKQKYDLICSACHQPNGTGLPPMYPPLKGSSVAVGKPISRHIELLL
                     NGVPGSAMQSYKDQLTDEEIAAIVTYERNAWGNNTNDLIQPADVAALRHGTDIKPTMV
                     NKAKAGGLQ"
     gene            10134..11750
                     /gene="ctaD"
                     /locus_tag="U6309_001723"
     CDS             10134..11750
                     /gene="ctaD"
                     /locus_tag="U6309_001723"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEN5505164.1"
                     /translation="MKYAIAHSEDSHEDHGPEQGKGFMGFIKRWLFTTNHKDIGTLYL
                     WLALISFFVAGAMALVIRLELFQPGHRFVDPNFFNQMTTLHGLVMLFGVVMPAFTGMA
                     NWQIPMMIGAPDMALPRLNNWSFWILPFAFCLLFSTMFHAGGGPNFGWTMYAPLSTTY
                     APPSTDFMIFSIHMMGLSSIMGSINIIATILNLRAPGMTLMKMPLFVWTWLITAFLLI
                     AIMPVLAGAVTMMLADRHFGTSFFNAAGGGDPILFQHVFWFFGHPEVYVLVLPAFGVI
                     SEIIPTFSRKPLFGYHFMVYATVSIAVLSFIVWAHHMFTTGIPLGAELFFMYTTMLIA
                     VPTGIKVFNWVSTMFKGAMTFETPMLFAIAFVFLFTIGGFTGLMLALVPADFQYQDSY
                     FVVAHFHYVLVPGVIFALVAATYYWLPKWTGHMYNERLGKWHFWLSVISVNLTFFPMH
                     FLGLAGMPRRIPDYALQFANFNMIVTVAAFAFGLSQLLFLYNVVATVRKRGTNKDVAT
                     SRVWEGSHGLEWTLPSPPPYHTFTTPPEIK"
     gene            11763..12305
                     /locus_tag="U6309_001724"
     CDS             11763..12305
                     /locus_tag="U6309_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEN5505165.1"
                     /translation="MIEEKKQKKLLTILTLLVLGMFAFGFALVPIYNSLCKNLGINGK
                     TKMEAVAYDEDKAKIAADREVLVEFVATNNSGVPWAFYPKTKKIKVHPGEIVKLAFYA
                     ENKTDHTMTVQAIPSVTPGIAAKYLKKTECFCFTQQTLNGHEAMNMPLLFHLDTDLPK
                     NVNTVTLSYTLFDVTGRSSN"
     gene            12464..13333
                     /locus_tag="U6309_001725"
     CDS             12464..13333
                     /locus_tag="U6309_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEN5505166.1"
                     /translation="MGAHGTYYIPKPSHWPLVGSIGLTTTLVGAACWLHHDWYGPYVF
                     IAGLCILVAMMFGWFGQVIYENSKGLYDLQVDRSFRWGMCWFIFSEVCFFGAFFGALF
                     FTRYWSVPILGGEMHPITHYTLWPEFKAIWPLLSNPNNQTFAGAHEAMGAWGLAAINT
                     IILLTSGVTITWAHWALKLRKRTQLLTGMSLTIALGVLFLMLQSYEYHEAYTEMNLTL
                     DAGIYGTTFFMLTGFHGLHVTIGTIMLIVILVRCKKGHFTPERHFAFEGVAWYWHFVD
                     VVWLFLFVFVYWL"
     gene            complement(13356..13628)
                     /locus_tag="U6309_001726"
     CDS             complement(13356..13628)
                     /locus_tag="U6309_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505167.1"
                     /translation="MLIQPIQPVAITIKIGVNNYDCQMISLSESELELKSNDYFEKES
                     QVTFIAKYFRGHAIVRELEFSDYCFTYKMEIVRIQFQPGLLINTRL"
BASE COUNT         3900 a         2636 c         2909 g         4229 t
ORIGIN      
        1 actccatgaa aatcatttaa caacaaaaat agcctataat tataatgtac aatatttttc
       61 aactgcataa cggatatcga tttatgcaag gagtgcgtta tgaaaatatt agtcaattca
      121 tccgttttgg caatcgtttt ggcgctttca acaccactat tcgctgatga tacgcaatgg
      181 atgacagagc ataatttggt gaaaggggtt atcacttttc aagatgattc gcaaattcag
      241 gtttatgctg ctcaagccga tcttgataaa ttgcaacaga atcttgctaa tattcaatcg
      301 gttgaagtac aacctaatca aaccgattct acaacgtcac caaatcaaca gagtgaataa
      361 cacgggtctt gtccgagtta aacgacatgt gctgtttcca gtttagctcg ggcgtgtgtt
      421 tttcttgtgg tcagagtttt ggttttctga atctgtctgc tgatattgct gatcagcctt
      481 ttccgctttg cgttggtttt catacagctg aatttcattg agttgttgct gccaaaatga
      541 agaagtatgt tcttttaatt gattgatttt tcgtacgagc atgccaaggt gggttgccag
      601 catgggaagc tttgatggcc caaacacaat gatagcaaca atgaaaataa gtaataactc
      661 accaagactc atgatggttt atgatcgtct ttttgagtgt catttgtttc ctcactattc
      721 atggctttgc ggaaattctt aatcgcttcg ccaaggtcag agccgattgt ttttaatttg
      781 gaagtgccaa acaaggctac aataatggct agaattaata agagtgataa cggacttatt
      841 ccacttaatc ccatgttagt tccttatctt gcggttgatg agtacaaaaa ttccagctaa
      901 aacagaacca gctattgtga tggttgtgag atgattataa tccagcaaat caaaatagct
      961 aatggcacct aatgataaaa atgagaaaaa gacacccagt cccacactct tccattggat
     1021 ggtctgattg ttattttcag attgcaagcg tgtgagttca tggactgtta acaactgatc
     1081 ctttttcatc tccagtatat caaaaatcag tttaggcata tgggggagtt gctcagtaaa
     1141 aaagggtaaa ttttgcttta agcgtttaat gaatgcttta ggtcccattt gttcgcgcac
     1201 ccatttttca aggaatggtt ttgctgtagc ccataaatcc aattcaggat agagttgcct
     1261 gcccaaacct tctatcgcca acagggtttt ttgcagcaaa atcagttggg gctgcacttc
     1321 catatgaaac cttctggcaa cctggaataa acggaaaact acttgcccaa aggaaatgtc
     1381 ttttaatggc ttttcaaaaa taggttcgca aacggttctt atgctgcttt caaattctgc
     1441 aacaggggtg tcacgcgata cccatccgga ttctacatgc aattcggcaa cgcgcttgta
     1501 gtcacggtta aaaaaagcaa ccaggttttc agccagataa cgcttgtcat tatctgtcag
     1561 agtcccaatg atgccaaaat caacacaaat atactgaggg tctttgggat tttggtagga
     1621 gacaaagata tttcccgggt gcatgtctgc atgaaaaaaa caatcccgga agacttgagt
     1681 aaagaaaatt tctactccac gctcggccag ttttttaatg tcaatgccgt gatctctaag
     1741 actggctata tcagtcacgg gtatgccatg gatgcgttca atgaccagga tgttatgatg
     1801 gcaataatcc caatagattt caggtatata taacatcgga gattgattga aatttcttcg
     1861 caattgagcg gcatttgccg cttctctcat taaatccagt tcatccagta aggtatgctc
     1921 aaattccttg actatctctt tgggtttgaa gcgctttccc tcaggccaat agcggtcagc
     1981 caatgtggcg atggtgtaca tgatacttaa atcttgttct ataagccggc gcatattggg
     2041 tcttagtatt tttacaacga cattttcccc tgtcttcaga gttgccgcat gcacttgtgc
     2101 catggaagcg gaagccagag cgatcggatc aaattgggca aaaacatcat aggcagactg
     2161 tttataggcc tgttcaatga tgttcatggc gacatggctg gggaatggag gaactttgtc
     2221 ttgcagttta cttaattctt ttgcgatgtc ttcaggaagt atatcgggac gtgtggaaag
     2281 agcttgtccg aatttaatga aaatggggcc gagttcctcc agcgatttgc gcagcgcttc
     2341 accacgcgtt aatttctctt tgcgaagcca gttccatggg tttaaataaa ctataaaacg
     2401 taaaggagca aacagtttga tagacacaac aacattatcg agaccatttt tggctaaaat
     2461 ataattgata tgtataagac gaataagttg cttaattgat ttcataacag ctcatcggtt
     2521 gttgatttcg agcttccagc cgctcaatat gcaatgataa tccatccaga tcctgaataa
     2581 aatcatccaa tcgtttaatt gccttcatca tcgctcatta aattattgat atgggcttga
     2641 aggcgatcaa cacttaacac caattcatca acctcggcaa aaaaatcttc caattcattt
     2701 cttgaaggag aaatgcgtaa ttcttcctga agaaattcgg tgatgttttg ttgcatcgag
     2761 gtgctgaatt gatttttaaa ttccagtcct ttacgtacaa aagagccaat ttgatgagcc
     2821 accacatcac ctgtgaagtg agccaggtgc ccttcccagt caatatctat ttcgtcaaat
     2881 agtttcttta cgctttgccc caattcaatg tcgcctgaga tgcgtatctt gtcattaaat
     2941 aaagagcggg ctttcgatgt gggtaataag ctcaagcgaa tcaatcctat cggattgctg
     3001 tgaatgattg tatcagggcg cccatcaatg cgatggagca attgcatttc actcccttta
     3061 aagcgaatgt aaaaattaac atttaagggg gtaatgacca tttcaagcgt tttgccgtcg
     3121 agtttttgaa gtttttgcgg catttgctca tcaagcttca tcgcctgatt gatggctgtt
     3181 tgtaatgctt tcagcgagta ttcttttaac ataatcaact caatatttgt aggcgatatg
     3241 cagagcgact ataccgccac ttaaattatg ataatgacaa tcctcaaatc caacccgttc
     3301 aatcattgct tttaatgttt cctgatcagg atgcattctg attgattctg caaggtattg
     3361 atagctggct tcatcgttag caacaaattt tccgattttt ggcaaaatgt taaatgaata
     3421 ccaatcataa acgggcttaa gtccaggaaa aactggtgtt gaaaattcaa gcaccattaa
     3481 tttcccaccc ggtttgcata ctctgtacat ggattgcagc gcttcgtctt tatcagtaac
     3541 atttctaagt ccaaatccca tagtgatgca atgaaaactg ttatccgcaa aagggaggca
     3601 ttgcgcatta ccctgaacat aacgaatgtt tttaaataat ccctcatcaa gcaagcgatc
     3661 gcggccaaca tggagcatgg ctgcgttgat gtcggcaaga ataacttgtc cggagtcgcc
     3721 cactttttgc aataataacc gggttaaatc accgctgccc ccggccaaat ccaatacgct
     3781 ctgcccaggt cgaacatgac ttaattcaat ggtatagcgt ttccatagat gatgaattcc
     3841 caaggacatc agatcattca tacgatcata atttttagca acagaatgaa atacttctgc
     3901 tactttcttt tctttctcat tccagtctac tgatttaaaa ccaaaatggg tggtttgttt
     3961 ctgattcgtc atatttgccg gatagtatca ttaaagtgtt catattaacc gataatcatt
     4021 aagataccca gttaaaaatt gtatactttt ttttatccat gtacagatga aaacaggggt
     4081 atttccagga tacacgctac aaattttgca aggtaattaa atagtggttt tcatctggcg
     4141 gcctattgtt tttttgggaa agccataagc attctgccaa ttgatccatg attaaatgtt
     4201 caacagccat gggatcctga tatttttcta ttaacttggt gtaaacagta cggatgcctt
     4261 gtggcctatc ggtcgctact tgttcgcgaa cggcaagatg aagccccata tgaaggaagg
     4321 gattggtttc gcctaattca ggataataag tatgttcagg aaaagatgga atttgttcaa
     4381 tgactttatg gtattccgga tgatcaagaa tcacttgggc aatttctttt tccaagggag
     4441 aaagttcttt tttattctgg tacttattcc agctgataaa aaatagctgt cgagtttctt
     4501 gtactgtatc gccgtaaaac ataatggccc gattgatata aaatgatcca ttttaactga
     4561 ataaaaaagt aacaacaatg ttgatgtgca aatatggtaa aaatgttgac attaatcagt
     4621 ggagtatgag atatttattt tgcgaggttg gacttgctat gtgtcattcc actgggtcaa
     4681 ttggcgacac actgattggc cctttctatt atcctaaaac cctgacatga actctctatg
     4741 gcttaatcta taagctgctt gtgattaatt tcatcgcaat ataagccatt aaaataccac
     4801 taagtaactc tattttttgc catactttat tttgggttaa taggtttgaa aaataacgag
     4861 tagtcatcgt taatgaactg aaccaaatca tgctggcaga aattacccct gctaaaaaag
     4921 ccagtttatg gtcaggatat tgactgctgc cgctgcccac aatcaccagg gtatctataa
     4981 tggcgtgagg attaagcaga ctaaacccca gggcaaataa aatgatttgc attcttgtat
     5041 gaggctgatg tgtttctaca acggtttgtt tgtttttgga caacgcgctt tttaagtttt
     5101 ttattgcata ataaattaaa aaggcagagc ctagccatac catccagatt tgcaagtttg
     5161 gatgagccaa aagcaattga tgtaatccag cgacactgcc gcacaccaaa atgacatcgc
     5221 agaaaaagca aatgacggca gagagagccg catgattttt tcgcgcacct tgcctgataa
     5281 gaaaaacatt ttgcggacct aaggccatta tcaatgataa tcccaagagt aatccattaa
     5341 aataaatcaa cataattgca tcaggacagt aaaaaaaagg cgattatatc ataattttgc
     5401 gcaagatgtt ttattgttga ttgatttcac atgctttctc caggacttta tgcaacatgg
     5461 gagcaacttt tgtgctggag taatgttgca ctctctgaaa tgctttgagc gcaagttgtt
     5521 ttgacaggtc tttgtctcgc aagaggcgtt ctaaaccatc cgccatgctg acagggtctt
     5581 ctgggggaac aaaaagagca cagcctgagt tacctgcaat ttcaagtggg ccggataagc
     5641 gagtgagaac catgggcaag gaatgcatca tgctttccag tacaaccagg ccaaaagatt
     5701 cttccctaga aggcaaacag aaaatatcaa tgcttttata aaatgactct ttatcctcaa
     5761 tccatccaag taaagtgatt tcatgctcca aaccatgatg acgaattaat tcgatgtatt
     5821 gttctttctc cttgccgtca ccagcaattt ttgcttgaaa agcaacacct ctctttttta
     5881 gttccgccaa agcgtttata aagacatcta ctcctttgat tgcagcaaat cgggcacaaa
     5941 caccaattat cgggatctgg ttgtttttag gttctctata ttgcaggccg tctggaatat
     6001 gaatcaaatt gggaatggta aatacccggc gctcctctat ccccgaattg ataatatccc
     6061 gacgcatttg ctctgtgagt gcaataatcg catcagtacc aaagtcatag tggcctttga
     6121 catggcacac agctatcttg gggactttgg tcctggtttt tttgaacaaa taggctgctc
     6181 tgtaactatg ggtaatgatg caatccggtt gatggatttt aattaatttt ttcaggcgat
     6241 gaattgcaaa aaaatcatgc tgattgaaat tatgcacggt gattaaatgg tcttgggggc
     6301 aactgttcct tatttctgct ttgggatgaa taattggaat gaccagattg ccttgagaac
     6361 ttaaagctgg aatatagttt aaaaatactt gttccaggcc tccgtttacc ttggagaaca
     6421 ttgcattaaa tattttcact ttaaattttc ctcattcaga gctgttcctc tattgatata
     6481 gaggggtagc aatgggaatg gctagcctac atcaaccaaa acttgcccat tttctatctg
     6541 ggttgggtat atttttaaat gcttccggga ggacacttta cccaaaagat tgccaacaac
     6601 aggaggccag ggggaccagc atttcgcttc gccggttttc aaatcaaatt cgcttttatg
     6661 aaaagggcaa atgatagaac aggattctgt aatttttccc ttggtcagtg gcaattttaa
     6721 atgcgggcac tgtgactgga tggcatgtac ttgattatca tgccagataa tcagtatttt
     6781 ttggccatca ataaccgcga cttctctgtt attttgctgc aatttgacta attctacggc
     6841 aggtttgaaa gacacgttaa ttacctctat attgaaaatc agaggattat atcagtgatg
     6901 aatttaatct gttagtagaa ggctgataaa ataaatcagt tgtgttatca aaattcctta
     6961 tttttgctga gttagcccag agtatcgtat caatgaccat acctgaatga tgtctttgag
     7021 cctgattatg gcatttcatg aaatgatgaa atttcgagaa aaggttgtgg gatataaggc
     7081 aggtgacagc tgggttggca taattataat ttttaaacag ggctgaattt aaacggttga
     7141 ataacgttgc aacttgataa gcgaggagta tacaatgcga caattattct gtctcctcat
     7201 gagagtcgtg aaaaataaga acaaatagga gggagcgtgg gctctttggt tataaaaaaa
     7261 tctattattg cggcgctagt tgttgcactg ggtattgcag ggcaatctgt acaagctgag
     7321 tctgtaccag atgaaacgtt acaatctgaa aaagtacaga cggaaaaagt tcaggccgaa
     7381 aaaacagaaa cagatttgtt ttctcccgta gagcagatca tgaatccttt ttatcccaaa
     7441 taccccccga ccaagccagc tactggcgag caagcagagt tgattaaacg tggtgagtat
     7501 ctggcaaaaa tgggtgattg cattgcctgc cataccgacg ttgaaggggg tactcccgct
     7561 tatgctggtg gtttacctat taatacgcct tttggcttat tctatagccc taatattacc
     7621 cccgataaag aaacgggtat aggaaattgg acagaaaaag attttattcg cgctcttaaa
     7681 gatggtcgtg atcctaaagg cagaaattat tttccagtat ttccatatgt ctatttttcc
     7741 aagataactg acgatgatgc tcgtgctctg tatgcttatt ttatgagcat tcctcccgtt
     7801 aagttaaaga acaaatcatt gccatttcct tttggtgtcc ccggggctcg ctttgcttta
     7861 tggggatgga acttgttgtt tttcttccct caggaaaatt ccattgaata tgatcctgat
     7921 aaatcccctg aatggaatcg tggaaggtat atagtcgata ctttagggca ttgcagtatg
     7981 tgccataccc caatgaatat atttggatca gcgaaaaaca gatactatct aaccggtagt
     8041 tttatcgatg gatattgggc tcccaatatc accaaatatg gattggaatc agcgagccat
     8101 caagaggtag ccgatgtatt taaggctggc gaattaatta ataaagcggg gcccgtagct
     8161 ggtcctatgg ctgaagtgaa tcataatagc ttgagtcact tgactgaaga agacaggttg
     8221 gctattgcaa cttatctcaa gacagtagag agtgaagaat accttggtgt ttctccttct
     8281 gatttgcctc ctagcgttgc acgtggaaga caagtgtatt tgaaggcctg tctgatttgt
     8341 cataaggaca ataaaatggg tgctccagtg attggtgatt cagccagctg gtatacacgc
     8401 ttgaaaaaca gcggtttaac tggtttatat cgacatgcca tttatggcta taaccaaatg
     8461 ccggttaaag gcgcttgtgt gacttgttcc gacaatgaca ttatttcagc ggttgattat
     8521 ttgctcaatg cgtccctgac ccggtctcag tggcaagatt tggcgtctgg cggtgttaaa
     8581 aaataccctg ccagtggaaa ggctgtatat aatgaaaact gtgctgtttg ccataatgaa
     8641 ggcaaattgg gtgctcctaa aataggcgat aaagtgacct ggaagccgct gatagcacaa
     8701 aatatggatg ttttgatcga aaacacaatc agagggaaaa atcatttgcc aaatggtggt
     8761 tgcaatcatt gttcgacaga tgaagtaatt gaagcgatca aatacatggt cagtcaatct
     8821 aaaaccgaag gtaattattc tttgtggtag ggttaccagc aacatttgaa taaatgaact
     8881 agcttaaaca ttagtcggtt tcccggctac ttgaagagat gagggattgg gatgttaaac
     8941 aggttaaata tctgtaaatt aattgcgtta tttagtgcct tgacttctgc gcagtcgctg
     9001 tgggctgctg cggatgagtg gcagttaaac atgtacaaag gggtgactcc tttaagcaac
     9061 gatatgtatt acctccatat ggtcgctatg gctgtttgtg cggttatagg agtggttgtt
     9121 tttggcgtga tgatctactc attaatccat cacagaaagt caaaaggata tcagccagca
     9181 agttttcatg ataatccaaa attggaaatc atctggacta ttatcccgtt tttaatcctt
     9241 gtcgggcttg cagttccagc tacccgagta ttaatccgta tggatgatac gcagctgtct
     9301 gatgtgacgg tcaaggtggt cggctatcag tggaagtggc agtaccagta tcttgatcag
     9361 ggtattagtt acttcagtag cttatcaaca ccctatgagc aaattgaaaa caaacaaaag
     9421 aaaaatcagt ggtacttgct ggaagtagat aatcccttag tcgtaccagt caacaaaaaa
     9481 attcgatttt tagtgacatc gaacgatgta gtccactcat ggtgggtgcc tgagctgggc
     9541 gtcaagcgag acgctatccc gggatttatg catgaggcat gggccagaat tgaaaagccg
     9601 ggtgtataca gaggtcaatg tgccgagctt tgcggaatta atcatggttt tatgcctatc
     9661 gtagtgaaag cagttagtga agaagaattc gatcgatggg ttagtgagca aaccaaagta
     9721 gaagacattt atgctgaaaa gtcggataaa ccgcgtgaac aaaaaacaat gacccgtgat
     9781 cagttaatga cgcttggcaa gcaaaaatat gatctgattt gctcagcttg tcatcagcct
     9841 aatggtactg gcttgccacc gatgtatcct cccctcaagg gtagttctgt tgccgttgga
     9901 aagccaattt cacgacacat tgaattactc ctcaatggtg ttcctggctc agcaatgcag
     9961 tcttataaag atcaactgac tgatgaggaa atagcagcaa ttgttacgta tgagcgtaat
    10021 gcttggggaa ataatactaa tgatttaatt caacccgcag atgttgcggc actgcgacat
    10081 ggtaccgata ttaaacctac catggttaat aaagctaaag ctggaggttt gcaatgaaat
    10141 acgcaatagc gcattcagaa gattctcatg aagatcatgg tcccgaacaa ggcaaaggct
    10201 ttatgggatt tatcaaaagg tggctattta caaccaacca caaagacata ggtactctgt
    10261 atttatggtt agcactgata agcttctttg ttgcaggagc aatggctttg gttatcaggc
    10321 ttgagttatt tcaacctggc catcgattcg tcgatcctaa tttctttaac caaatgacaa
    10381 cattgcatgg attagtcatg ctgtttggtg tcgtcatgcc agcgtttacc ggtatggcaa
    10441 actggcaaat ccccatgatg ataggtgctc ccgatatggc tttgccaaga ctaaacaact
    10501 ggagcttctg gattttacca tttgcttttt gtctgttgtt ttctaccatg tttcatgccg
    10561 gtggcggtcc aaattttggc tggaccatgt acgcgcctct ctctacaact tatgcgccac
    10621 ccagtactga tttcatgatt ttttccattc atatgatggg actctcatcc atcatgggat
    10681 caattaatat catagccacg attttaaacc tgagggctcc gggtatgacc ctgatgaaga
    10741 tgcccctatt cgtctggacc tggcttatta cggcgttcct tctgatcgcc atcatgcctg
    10801 ttctggcggg tgcagttact atgatgttag ccgatagaca ctttggcact tctttcttta
    10861 atgcggcagg tggaggagat cctatcctgt ttcaacacgt gttttggttc tttggtcacc
    10921 ctgaagtgta tgttctggtc ttgccagctt ttggggttat ttcagaaata attcccactt
    10981 tcagccgtaa acccttattt ggttatcatt ttatggttta tgccacagtg agcatcgctg
    11041 tattatcctt tatagtttgg gctcaccata tgttcaccac gggaattccc ctgggggctg
    11101 aattattctt tatgtacacc accatgctga ttgcggtacc taccggtatc aaggtgttca
    11161 actgggtgag tacaatgttc aaaggggcta tgacctttga aacgccaatg ctgtttgcca
    11221 tagcgtttgt atttttattc actataggtg gatttacagg cttaatgttg gcattggtac
    11281 cggccgattt tcaatatcag gacagctatt ttgttgtggc tcacttccat tatgttcttg
    11341 tgcccggcgt tatctttgct ctggtagctg cgacttatta ttggttaccc aagtggactg
    11401 gacatatgta caatgagcgt ttgggtaaat ggcatttttg gctttctgtc atttcagtta
    11461 atttgacttt tttccccatg cattttctgg ggctagctgg tatgccaagg cgaatccctg
    11521 actatgcttt gcagtttgcc aattttaata tgattgtaac agtggctgct tttgctttcg
    11581 gtttgtcgca attgttgttc ctgtacaatg tggtagccac agtaagaaaa agaggcacta
    11641 ataaggatgt agcaacgagc agggtttggg aagggtcaca tggattggaa tggactttgc
    11701 catctccacc accttaccat acgtttacca ctccacctga gattaaataa aggtggagtt
    11761 tgatgattga agaaaaaaaa cagaaaaaac tgctcaccat attaacatta ttagtattag
    11821 ggatgtttgc ttttggtttt gctctggtcc ctatttataa tagcctatgc aagaatctgg
    11881 gcatcaacgg caaaaccaaa atggaggccg ttgcttatga tgaagacaag gcaaaaattg
    11941 cagcagatag agaagtcttg gttgaattcg tagcaaccaa taacagtggt gtgccttggg
    12001 ccttctatcc caagacaaaa aaaattaaag tgcacccagg agaaattgta aagctggcgt
    12061 tctatgcgga aaataaaaca gaccatacaa tgacagttca ggcaatccca agtgtgacac
    12121 caggaattgc agcaaaatat ttaaaaaaaa cggaatgttt ttgttttact caacaaacgc
    12181 taaatggcca tgaggcaatg aatatgcctt tactattcca tttagatacg gatttaccga
    12241 aaaatgtgaa tacggttact ctgtcttata cattatttga tgtgacaggc agatcaagta
    12301 attaggcgtc gttgatatgt ctacctttgc ggctggcttg cctcgatttt ctgaacgttg
    12361 tttcttgaaa tcgtgacaat gcaattcacg ctagccaaat atcaacggtc tttgagtatc
    12421 agctttggct gaatgactta ttgttacagg agattaaagc ataatgggag cacacggtac
    12481 atattatatt cccaaaccca gtcactggcc tttggttggc tcaataggtt tgaccactac
    12541 actggttggt gcagcatgct ggctacatca tgactggtat ggcccttacg tatttatagc
    12601 gggcttatgt attttggttg caatgatgtt tggctggttt ggacaagtca tctatgaaaa
    12661 cagcaaaggc ctatatgatt tgcaagttga tcgttcattt cgctggggta tgtgctggtt
    12721 catattttct gaagtatgct ttttcggagc ttttttcgga gccctgtttt ttacaagata
    12781 ttggtcagtt cctattttgg gtggtgagat gcatcctatt actcattaca ccttatggcc
    12841 tgagttcaaa gcaatttggc ctttgctgag taacccaaat aatcagactt ttgccggggc
    12901 tcatgaagcc atgggagcat ggggtctggc tgcaataaac accattattt tattaacttc
    12961 cggggtaacc atcacctggg cacattgggc tttaaaatta agaaagcgga cccaattact
    13021 gacaggtatg tcattaacta tcgctttggg cgttttgttc ttgatgctac aaagctatga
    13081 gtatcatgaa gcatatacgg aaatgaattt aacgctggat gcaggtattt atggtactac
    13141 tttttttatg cttacaggat tccatggttt gcacgtaaca atcggaacaa tcatgcttat
    13201 cgttattctg gttcgttgca agaagggtca ttttactcct gaacggcatt ttgctttcga
    13261 aggggttgcc tggtattggc actttgtgga tgtggtgtgg ttgttcctat ttgtctttgt
    13321 ctattggcta tagagctaat ctaattcatc ctttattaca accgggtgtt gataagcaag
    13381 cccggttgaa attgaatacg aacaatttcc attttataag taaagcagta atctgaaaac
    13441 tctaattcac gaacgatggc atggcctcga aaatattttg caataaaagt aacctgactt
    13501 tctttttcaa aataatcatt actttttaat tccaattccg attcagacaa gctaatcatt
    13561 tgacaatcat aattattcac accaattttt attgttatag caactggttg aataggctga
    13621 attaacacgg cttcccctaa tttctactga attgtgtgct aataaatcaa aatg
//
LOCUS       DASLOE010000077        13467 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.077, whole genome shotgun sequence.
ACCESSION   DASLOE010000077 DASLOE010000000
VERSION     DASLOE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13467
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.077"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(238..4557)
                     /locus_tag="U6309_001727"
     CDS             complement(238..4557)
                     /locus_tag="U6309_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEN5505168.1"
                     /translation="MIKSKTFSCYIIGEANITLQCADILLAGGHKLLGIISPSNTIKK
                     WCTANSIAYLENIKEFEKNHMDEEFDFLFSIVNSEIIPQKILRLPRYYAINYHNSPLP
                     KYAGLYATSWAILNGEIQHGISWHIMNEVIDAGDILKQPTFPINDQDTAFSLNLKCYE
                     QGIYSFHELVEELSSHTTTLVKQNLSCRSYYGLKNKPANFGFISWEESSESIDRLCRA
                     LTFGNYTNQLEVPKIMINKEIYVVKSYEKLNISSGVKPGTIVNNSNGGLQVATGTTDI
                     LILELTDLEGRTCSMEQLGSLFKMTCYSQLDTIEPAFFEKLTICPAKKPKIEKFWVNE
                     FLKCAQEKNSFLSPLSQLDKTSCSLHDRKTVTQVPGELLAKLKKLCPEHWQIKNILLT
                     GLLIYLYRLNNYSNLSIAFINSQLSSNDPDLNKLLSDYVPLTTQFDSTMTFIDALTFV
                     SKEQSTLYENSTYTRDIFIRYPELHNTFNHIDVSITFITDSKAVAPCVGNKKLNFCIA
                     EDCSWFYVNNKTNYRSHLESYAFFENMNEHLLTLLEDIVSNPDKKIFELSIISKKEKN
                     DLLVVWNNTQCDYDYEKPIHQYFEEQVSKTPEEIAAVFEDKSITYAELNRKANQLAHY
                     LRSQGVKQDDLVGISLSRSLEMVICILGILKSGGAYLPLDPNYPDERISYMLMDSKTN
                     LLITDQEFIKRKPNGFNGKSIEINSFLNLKNLSSENLQAINKPSDLAYIIYTSGTTGK
                     PKGVAISHRSICNHMLWMKKEYAFKNKDVFLQKTPFSFDASVWEFFMPLLVGGKLVVA
                     PNDAHTSPNQMIRLIRENKVSVLQIVPSMLKELVSNEEFGLCKSLTHVFCGGEALLSE
                     TINAFFKYNFSDTKLHNLYGPTEVTIDTTAYTCTAGDAKGDVSRIGKPIMNTKVYVLD
                     TKMQPVPVGIMGELYISGDGLARGYLNNPEFTIQKFLPNPFSNNKNDRLYRTGDLVKW
                     DSNGVLEYHGRCDNQVKIRGYRIEINEIESYLEKIPSIHQCIVKPEKNQDDSMSLSAY
                     LVLEKNSQISAVDIRTILKQNIPEYMIPARFYIVDKFFSTPSGKIDRKMLPIPSKRLR
                     SGTNYAPPNNSTEQLLHNIWCSVLKIDNLGIYDDFFELGGNSLSAMNIISHIREQFSL
                     TLSVRTLFDFPTISSLSKEIENIYHKKTDCIYNQSSGNNIIPLRTEGEKTPLFLVHPI
                     GGSVFWYKLLGQYFDKDRPLYGIQDPGLDRNELIFSNLEEMASAYVHSIQAIQPSGPY
                     ILGGASFGCTVAIEMAKQLEEKGETVISIISLDGWAFYPSLQNNELYFQEVMKEQNSR
                     ILKKYIENNVSNSEFLLELQWHRENMLTQYKMPIIKPKFILFKAKKLTEMFQYNASLN
                     WWENYTSQPIELHLTPGDHESMFYEPNIKILATKLNDSLNEQDIKHYQFNDTMKDSFI
                     DQL"
     gene            complement(4719..7871)
                     /locus_tag="U6309_001728"
     CDS             complement(4719..7871)
                     /locus_tag="U6309_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEN5505169.1"
                     /translation="MKIALFFIDRPVFATVISIIIVMVGVLSYFNLPIEQYPQVVPPT
                     IHVYASYPGANAKTVAETVATPIEQEVNGVENMLYMDSQSTDDGQMRLTITFKLGTDL
                     NQAQVLVQNRVAVVEPKLPEEVRRLGITTNKNSPDLMLVVNLYSPNGQYDQTYIGNYA
                     ILHIRDKIRRIEGVGNVLIFGASEYAMRIWLNPDLINTFNLTANDVLAAVRAQNVQVA
                     SGKLNLQPQKNQYGFEYHIETKGRLVKKEEFENIIVKSGEGGRIVRLKDIGRVELGTQ
                     NYLTKGYLDKYPAVALPIYQRPGTNALATAQEIIKTMKTIAKDFPPGITYDIAYNPTL
                     FVQQSIDAVFHTIYEAIALVVLVILIFLQTWRASAIPVVAIPVSLIGTFAVMQAIGFS
                     LNYLTLFGLVLAIGIVVDDAIVVVENMERNIQSGMNPRDAARKTMTEVGSALVAMGLV
                     LIAVFLPTVFLEGISGRFYQQFGTTLAVATAISVFVSLTLTPTMAVLLLRAHHETIKQ
                     EGLAWWRKPIYGFLHGFNRLMEGFSRQYGKLVATLTRKTALMIIAYGIFISITLLLFV
                     YVPRGFIPRQDQGYFIVAVQLPPGASLSRTDAVINKAVKKILAIPGIAHTVSFTGFSG
                     ATFTNSSNAGAIFTPLLSFQERQRMGINYNDILKRLRQELSTIKEALIVVIPPPPVRG
                     IGNAGGFKMMLQDRGGRGLDVLMEAAATMVNAANQEKATTSVFTFFENSTPRLHLKLD
                     REKVERLNVPYTNVVEALEVYLGSVFINEFNYLGRTFRVIAQADSEYRHTEDDILRIK
                     VKSNNGDMVPIGSVAQIENTVAPSRMPRFNLYPAIDLQGDVAPGYSSDEALATMEKLA
                     QNNLPDGIGYEWTEIAYQQKMVGNTALMAFTLGVIFIFLVLAAQYESWSLPLAVILIV
                     PMCLFSSMLGVKILGMENNIMTQIGFLVLIGLASKNAILIVEFARQLENRGYNLWKAA
                     IQAAKLRLRPILMTSFAFILGVFPLIIATGAGAEMRRALGVAVFSGMLGVTFFGLVFT
                     PLFYVLISRLSRYKRKLNRP"
     gene            complement(7971..9077)
                     /locus_tag="U6309_001729"
     CDS             complement(7971..9077)
                     /locus_tag="U6309_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEN5505170.1"
                     /translation="MKEILTLVMRLLAVILIPALMSCNSESNKPVGQTLAEVDVALPV
                     KKNIVEWDEYTGRFQAVEEVDVRARVTGYLEAIKFQDGQTVKKGDVLFVIDQRPFKYA
                     LARAEAQYALAKAQYERAIKLQKEKFISAEVIDQRFQDVQVAETRVQEAKLNLEFTEI
                     KTPISGKISRDFVSVGNLIRMNDTVLTRVVSVDPIYFYFETSQNDLLKYIRLDRAGKR
                     LSSEKKPTPVLIKLPDEKDYLHHGTVDFLDNVIDSGTGTILARALVPNPDNVIYPGLF
                     ARVKLVGSGEYEAILLPDKAINTDQARKFVYVVDNENKVKRVYVELGPLRESGFYIIR
                     NGLKGGEKVIIHGIQRIHGPNQEVKPVLIEVIEN"
     gene            9290..11128
                     /gene="legS2"
                     /locus_tag="U6309_001730"
     CDS             9290..11128
                     /gene="legS2"
                     /locus_tag="U6309_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector sphingosine 1-phosphate
                     lyase LegS2"
                     /protein_id="HEN5505171.1"
                     /translation="MFVRICAMFGFISDLLTAAVSSLDELLQDTPAHQIILGTAALYF
                     LYNQYHNPSISRWYRSRNNASMKQRIIDSAYALAKNLPGVNQIIEKELNKELSSTREK
                     LRIQRSGMTLREEIPEEGLSPQDILSAFDVDVEKCHFDFLSVTNDSTEKEFLVGRGDG
                     KDSGALYAIHPKELTELLKEVYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKE
                     GYGLLTHGGTTSIIEAMAAYVIRARAKGIDYPEIVVPETAHAAFKKAAELTGAILITV
                     PVDKKTGAVNPNVMSSYITRNTAVMVGSAPSFMNGIHDPVSELGQLAKKKNVPFHVDA
                     CLGGFLTAFLDTSSQPMDFRVPGVTSISADLHKYGCCPKGTSVCLFSEDSPALSVYAA
                     LNWSGGLYATPGILDGSTSGARVAEVYATLSYYGKNKYQEIAKSIIRLRNAIQKELKA
                     LVEEGNGLTSEDIYVYGNPQWSILGFRSNTYNAHFIADELEKRGWKLNLLQNPDGFHL
                     CLTHVHTLVRGFETQFIKDLREAVIDVKNYPPGKKPSGNVKVYGAVGMMPIELQKEIC
                     KQYQKARLDFTAANHGSLGIFTPSSTEEDDGLRNRKVGEQKVQTSL"
     gene            complement(11264..12025)
                     /locus_tag="U6309_001731"
     CDS             complement(11264..12025)
                     /locus_tag="U6309_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN5505172.1"
                     /translation="MLKLFDAHFHIIDYRFPLVANQSYLPPEFTVEDYLQRAKPLNIC
                     GGAVVSGSFQAFDQTYLLTALSVLGPNFVGVTQLPATVSDEEIIQLNQQGIRAVRFNL
                     KRGGSENIHQLKYFAHRIYEMVRWHVEIYVDSKELGDLTNLLLELPAVSIDHLGLSQS
                     GFSELLHLVEHGIKIKASGFGRVDFDVKKALQTIAQINPDALLFGTDLPSTRAPRPFL
                     DSDIQLIRDLFDEELTEKILYRNACNFYSKSEPLT"
     gene            12202..12274
                     /locus_tag="U6309_001732"
     tRNA            12202..12274
                     /locus_tag="U6309_001732"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12235..12237,aa:Lys,seq:ctt)
     gene            12392..>13467
                     /locus_tag="U6309_001733"
     CDS             12392..>13467
                     /locus_tag="U6309_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEN5505173.1"
                     /translation="MHQELLKYLEMYHPQIAPFMHLQINEWGKNRPLGGVKILHHVPL
                     VPNTLLKIAALVAANAEVTVTNPASFCQAHPDAITCLHKSGIPYVENLDLLRDETFDV
                     YLDCGGQLHQSLGRPLRGAIELTGSGDQYYRSLKTLDFPVISIDHTMTKQLETVFGCA
                     ESSHQAIQKLTGVDPTNKNWLIFGFGKIGRGLAYFCLEHQVPVTVVDSSVHQCDLASN
                     LGIEAIRPEEHDKLEKAVAKADIILTATGGKNIMSKYPRSWFDGKILGNLGLHDEFGP
                     NFTSQDVLYDKKPINFCLYDPTPMIFIDPEFYLHNLGCLLLLQGNFPRGMHDIPEDID
                     SSIIKDWCQYHRRSEKSIRRWFLTS"
BASE COUNT         4031 a         2533 c         2644 g         4259 t
ORIGIN      
        1 gaatattttc ttggcggaag cgttgagatc taaaataata aaatctgagg aaagataaaa
       61 tattggttct tcaacggtat tgatgaggag caatgtcaga tccgtttttt taggcataga
      121 gagtgaccta attcaatttc attcatatgg attttattta ttccatttaa ataataggtc
      181 acggacagag aagaaacaaa tcaaccaaaa cctctgtgca gttctgatga tactggttta
      241 gagttgatct ataaatgaat ccttcattgt atcattgaat tggtaatgct taatatcctg
      301 ctcatttaag ctatcattga gcttggtagc caggattttg atattaggct cataaaacat
      361 gctttcatga tcaccaggag ttaaatgtag ttcaattggt tgacttgtat aattttccca
      421 ccaattcaaa ctagcattat attgaaacat ttctgtaagt ttttttgcct taaataaaat
      481 aaatttgggt ttaattattg gcattttata ttgagtcaac atgttttctc tatgccattg
      541 taattctaat aaaaattcgg agttactgac gttattttca atatatttct ttagaattct
      601 tgaattttgt tctttcataa cttcttgaaa ataaagctca ttgttttgta aggaagggta
      661 aaatgcccaa ccatcaaggg agataataga aattactgtt tctccttttt cctctaattg
      721 tttcgccatt tcaatagcaa cagtacatcc aaatgatgcg ccgccaagta tgtaaggtcc
      781 gcttggttga atagcctgaa ttgaatggac atatgcacta gccatttctt ccaagttgct
      841 aaatatcaat tcatttctat caagcccagg atcctgtatt ccatacaatg gtctatcttt
      901 atcaaaatat tgtccaagaa gtttgtacca aaaaacactt ccaccaattg gatgtactaa
      961 aaacagaggt gttttttctc cttctgttct aagagggatg atattatttc cagaagactg
     1021 attgtatata caatcagtct tcttatggta tatgttctct atttctttag ataaagagga
     1081 tattgtggga aaatcaaata atgttctaac gctcaatgtc agtgaaaatt gttcccgtat
     1141 atgtgatatt atgttcattg ctgatagtga attaccgcca agttcgaaaa aatcatcgta
     1201 aattcccaaa ttatctattt ttaaaaccga acaccagata ttgtgtaata attgttctgt
     1261 agaattattg gggggagcat aattagtacc cgaacgtaat cgtttggatg ggattggtag
     1321 catttttcta tctattttac cgcttggggt gctaaaaaat ttgtcaacaa tataaaatcg
     1381 ggccgggatc atgtattcag gaatgttctg ctttaatata gtccgaatat ctacagcgga
     1441 tatttgagaa tttttttcca aaaccaaata agctgataaa gacattgagt cgtcctggtt
     1501 tttttccggt ttcacaatgc attgatggat tgaaggaatt ttttccaaat aggattcaat
     1561 ttcattgatt tctatccgat atcctctaat ttttacttga ttatcacatc ggccgtgata
     1621 ttccagaact ccgttggaat cccatttgac aagatcccct gttctgtata atcgatcatt
     1681 tttattgttg ctgaaagggt tgggtaaaaa tttctgtatg gtaaattcag ggttgtttaa
     1741 ataaccacgt gctaaaccat ctcctgaaat ataaagctcc cccattattc caacaggaac
     1801 aggctgcatt tttgtatcta atacatacac ttttgtgttc attatcggtt tgcctattcg
     1861 gcttacatcg ccttttgcat ctcctgcggt acatgtataa gcagttgtat caatggttac
     1921 ttccgttggt ccatagagat tatgtagttt tgtatctgag aagttgtact taaaaaaagc
     1981 attaattgtt tctgataaaa gggcttcacc accacaaaat acatgcgtga gtgatttgca
     2041 gagaccaaac tcttcattag atactaattc ctttagcata gaaggaacga tctgcaatac
     2101 gctaactttg ttctctctga ttaggcgtat catttgattg ggactggtat gtgcatcatt
     2161 tggggcaacg accagtttac caccaaccag aagtggcata aaaaactccc aaacagaggc
     2221 atcaaacgag aagggggttt tctgtaaaaa tacatccttg tttttaaaag catattcctt
     2281 cttcatccac aacatgtgat tgcaaataga acgatgcgat atggctacac ctttaggttt
     2341 tcctgtagtc ccggaagtat aaataatgta ggctaagtcg gatggtttgt taattgcttg
     2401 gagattttca gaagataggt tttttaaatt taaaaaggaa tttatctcaa tacttttgcc
     2461 attgaatcca tttggttttc tctttatgaa ttcttgatcc gttatcagca ggttagtttt
     2521 actgtccatt agcatgtatg aaatccgctc atcaggataa ttaggatcta atggcaggta
     2581 agctcctcca gactttaaaa tgcctaaaat gcatataacc atttccaggc tgcgacttaa
     2641 agaaattcct accaggtcat cttgttttac accttgagat cttaaatagt gtgctaattg
     2701 attggctttt cggttaagtt ctgcataagt tattgatttg tcctcaaata cagcagctat
     2761 ttcttcaggt gttttggaaa cttgctcttc aaaatattgg tgaatcggtt tttcataatc
     2821 gtaatcacac tgagtgttat tccagactac caataaatcg tttttttcct ttttactgat
     2881 tattgaaagt tcgaatatct ttttatcagg gttgctgact atatcttcca ataaagtcag
     2941 caaatgctca ttcatatttt caaaaaaagc ataagattcc aaatgggatc tataatttgt
     3001 tttattatta acataaaacc aggaacaatc ctctgcaata cagaagttta actttttatt
     3061 gccgacacaa ggtgccactg cttttgagtc agtaataaag gtgatactga catcaatatg
     3121 gttgaaagta ttatgtagct cagggtagcg tatgaaaata tctctagtgt aagtgctgtt
     3181 ttcatataac gtgctctgtt cttttgagac aaatgtcaat gcgtcgataa aagtcatagt
     3241 gctatcaaat tgcgttgtta aaggcacata gtctgataaa agcttattta aatcaggatc
     3301 attactgctc aattgtgaat taatgaatgc aattgagaga ttactgtaat tatttaatcg
     3361 atataggtaa attaagaggc cagttaataa gatattcttt atttgccaat gctcagggca
     3421 tagctttttc aattttgcga gtaactcacc aggtacttgt gttactgttt ttctatcgtg
     3481 aagtgaacat gaggttttat cgagttggga tagtggagat aaaaatgaat tcttttcctg
     3541 agcacatttc aaaaactcat tgacccagaa cttctctatt tttggctttt tagcaggaca
     3601 aattgttagc ttttcaaaaa acgcaggttc aattgtgtca agttgtgagt agcaagtcat
     3661 tttaaataag ctgcctaatt gttccattga gcatgttctg ccctcaagat cagtaagctc
     3721 tagaatcaat atatctgttg ttccggtagc tacttgtaag ccaccattcg agttattcac
     3781 aatggtgcct ggttttactc ctgaggatat atttaatttt tcataagatt ttacaacata
     3841 aatttcctta ttaatcataa tcttgggtac ttctaactga ttggtataat ttccaaatgt
     3901 taaagccctg cagagccgat caatactttc tgaagattct tcccatgaaa taaatccaaa
     3961 atttgccggc ttgtttttta gaccataata agaacggcat gacagatttt gtttaaccaa
     4021 agtcgtagtg tgggatgaaa gctcttcaac aagttcatga aaggaataga tcccttgttc
     4081 ataacatttc agatttaaac taaacgccgt atcctgatca tttattggaa aagttggttg
     4141 ctttagaata tcacctgcat ctatcacttc attcataatg tgccaactaa taccatgctg
     4201 aatttcgcca ttaaggatcg cccacgaagt ggcatataat cctgcatatt tgggtaatgg
     4261 ggagttatga tagtttatgg cgtaataacg agggagtctt aaaatttttt gaggaatgat
     4321 ttcactgttg actatgctaa acaagaaatc aaattcttcg tccatatgat ttttttcaaa
     4381 ttccttgatg ttctcgagat aagcaatgga attggcagta caccattttt ttatggtgtt
     4441 tgatggagag attatgccaa gtaatttatg gcctcctgct aataggatat ctgcacattg
     4501 aagagttata ttagcttcac cgataatata gcaactaaat gtcttgcttt taatcatagt
     4561 gctccatgct gtcagactgg atcgattaat aaaagccaca ttttaattga aatgatcttt
     4621 tttagtcaag gttgactaaa atttcactca tgacatcatg caaagaacag aggaaccgag
     4681 tgtataaata cattaagggt ttcacagcaa atatttagtt aaggtctgtt taatttcctt
     4741 ttatatctac tcaagcgact aattaaaaca taaaataaag gcgtaaagac caggccaaaa
     4801 aaagtcactc ccaacatccc gctgaataca gcgacaccga gtgcacggcg catttcagcg
     4861 ccagcccctg ttgcgataat caagggaaaa acaccaagaa taaatgcaaa tgaggtcatc
     4921 aaaatcgggc gcagacgaag ttttgctgcc tgaatggcgg ctttccagag attatagccc
     4981 cgattttcaa gttgtctcgc aaattccaca atgaggatgg catttttcga tgcaagtcca
     5041 atcaaaacca gaaaaccaat ttgagtcata atattatttt ccatcccgag aattttaact
     5101 cccagcatag aagaaaagag acacatcggt acaattaata tgaccgctaa gggtaaagac
     5161 cagctttcat attgagcggc aaggacaagg aaaatgaaaa taacacccag agtaaaggcc
     5221 atcaatgcag tattacctac cattttctgt tgataggcta tttctgtcca ctcataacca
     5281 atcccgtctg gaagattgtt ctgagccagt ttttccatgg tcgccaacgc ttcatcagaa
     5341 ctgtatccgg gagcaacatc accctgcaaa tcaatagcgg gatacagatt gaaacgaggc
     5401 atacgcgagg gagcaaccgt gttttctatt tgcgcaaccg agcctatagg aaccatgtct
     5461 ccgttgttgc ttttgacttt gattcgcaaa atatcatctt cagtatgccg atattctgaa
     5521 tcagcttgcg caataacacg aaaagtacgt cccaggtaat tgaattcgtt aataaaaaca
     5581 gagccaaggt aaacttccag tgcttccacg acattggtgt aaggcacgtt aagtcgttcc
     5641 accttttctc tgtcaagttt caagtgaagt cgtggtgttg aattttcaaa aaaagtaaat
     5701 actgatgtgg tggctttttc ctgattcgct gcattaacca ttgttgctgc cgcttccatt
     5761 aatacatcaa gacctcgacc acccctgtct tgaagcatca ttttaaaacc acctgcattt
     5821 ccaatacctc gaactggggg aggagggata accacgatga gggcttcctt gatggtactt
     5881 aattcctgcc ttagacgctt aagaatatcg ttataattta tccccatacg ttgcctttct
     5941 tgaaatgaca gtaggggggt aaaaattgct ccggcattcg atgaattagt aaatgtagct
     6001 ccagaaaagc cggtaaaact aacagtatgt gcaatgccag gaatcgccaa tattttcttg
     6061 accgctttgt tgatgacagc atcggtacgg ctcaaagaag ctccgggcgg caattgaact
     6121 gcaacgataa aatagccttg gtcttgtctt gggataaagc cgcgtggcac gtaaacaaat
     6181 agaagtagtg tgatggaaat aaaaatacca taagcaataa tcattaaagc cgttttacgt
     6241 gtgagggtag caaccaattt cccatattgc cgggaaaatc cttccattag tcggttaaat
     6301 ccatgcaaaa atccatatat tggttttctc caccaggcta atccttcttg ttttatagtt
     6361 tcatgatgag cgcgaagcaa taataccgcc atggttggag tcagggttaa cgagacaaaa
     6421 acagaaatgg cagtcgcgac agcaagagtt gtaccgaatt gttgataaaa acgcccggaa
     6481 attccttcca gaaaaacggt aggtaaaaat acggcaatca acaccaaacc catagcgacc
     6541 aatgctgatc ctacttctgt catggttttt ctggcggcat cgcggggatt catgcctgac
     6601 tggatattac gctccatatt ttcaacaacg acaatggcat catcaaccac aataccgata
     6661 gcaagaacaa gtccaaacaa ggttaagtaa ttcaacgaaa atccaatagc ctgcatcacg
     6721 gcaaaagttc ctatcaatga tactggaata gcaactactg gaatggcaga ggctctccat
     6781 gtttgtagaa aaatcaaaat gacgagtaca accagagcaa tcgcttcata aatggtatga
     6841 aataccgcat caatagactg ttgaacaaat agagtaggat tataggcaat gtcataggta
     6901 atgccaggcg gaaaatcctt ggcaatcgtt ttcatagttt taatgatttc ttgcgcggtg
     6961 gctaatgcat tggttcctgg gcgttggtaa ataggtaatg caacagcagg gtatttgtct
     7021 aaataacctt tcgtcaggta gttttgcgta cctaactcga cgcgaccaat gtctttcagg
     7081 cgaacgatac gcccgccttc acctgatttg acaataatat tctcaaattc ctctttttta
     7141 accaagcgtc ctttggtttc aatatggtac tcgaaaccgt attggttttt ctggggttgc
     7201 aaattaagtt taccgctggc gacttgcacg ttttgagcac gaacagctgc taaaacatca
     7261 tttgcggtta aattaaaagt atttattaaa tcgggattga gccaaatacg catggcatat
     7321 tcactggccc caaatatgag tacattgcct acaccttcaa ttcgtctgat tttatcgcgt
     7381 atatgcaaga tagcataatt gccgatgtag gtttgatcat attgaccatt aggggagtat
     7441 agattcacta ccaacatcaa atcaggtgaa tttttattag tagtgattcc taagcggcgg
     7501 acttcttcag gtaatttggg ttccacaaca gccaccctgt tttgcaccag tacctgcgcc
     7561 tgattcagat ctgttccaag cttaaaagta atagtcagac gcatttgtcc atcatcagtg
     7621 gattgggaat ccatgtaaag catgttttca accccgttaa cttcctgttc aatgggggtt
     7681 gccacggttt cagctactgt ttttgcattg gcgcctggat atgaggcgta tacatgaatc
     7741 gtggggggaa ctacttgcgg gtattgttct attggtaagt tgaaatagga caataccccc
     7801 accataacaa ttatgatgga gattactgtt gcgaatacag gtctatcaat aaagaaaaga
     7861 gctattttca tacagtgaat ccttttcgtg ttggctacac tgtaaaacgt tgtatgcttt
     7921 atgtagtatt aaattctatc gaatgagcat gattccaaca cacaactggt ttaattttca
     7981 atcacctcaa tcaacacggg cttaacttcc tggtttgggc catgaatacg ttggattcca
     8041 tgaatgataa ctttttcacc ccctttcaat ccatttcgga taatgtaaaa cccgctttcg
     8101 cgcagtggcc ctaactcaac atacactcgt ttcactttat tttcattatc aaccacatac
     8161 acaaatttgc gggcttgatc tgtattgatt gctttatcgg gaagtaaaat tgcttcatat
     8221 tccccacttc caactaattt tacacgtgca aacaggccag gataaatgac gttatcaggg
     8281 ttaggaacaa gtgctctggc aagaatagtt cctgtgcctg agtcaatcac attatctaaa
     8341 aaatccactg ttccatgatg caggtaatcc ttttcatcag gtaacttaat taagactgga
     8401 gttggttttt tctcggagct gagtcttttg ccagcacgat cgagacgtat gtatttgagc
     8461 aaatcatttt gactggtttc aaaataaaaa taaatgggat caacagaaac tactctagtc
     8521 aagactgtat cgttcatgcg gattaaatta cctacactca caaaatcgcg acttattttg
     8581 ccactgatgg gggttttgat ttcagtaaat tcgagattca attttgcttc ctgtactctg
     8641 gtttcagcaa cttgtacgtc ttgaaatcgt tggtcgatca cctcagcgga aataaatttc
     8701 tctttttgta gttttatggc acgttcatat tgtgctttgg ctaaggcgta ttgagcttct
     8761 gctcttgcca gagcatattt gaaggggcgt tgatcgataa cgaataaaac gtctcctttt
     8821 tttaccgttt ggccgtcctg gaacttgata gcctcaagat atccagtgac acgggcacga
     8881 acatccactt cttctactgc ttgaaatcgt ccggtgtatt cgtcccattc tacaatattc
     8941 ttttttactg gtagcgcgac gtcaacttct gccaaggttt gtcccactgg cttattggat
     9001 tctgaattac aggacatgag ggcaggaata agaataacag cgagtaatcg catgaccaat
     9061 gtcagtattt ctttcattgc tatctatccc tgatattcaa ttcattggta agttatgatc
     9121 cagtggataa gcttagttta gcttataaag ccgcattatg cgtaattcga atattatatc
     9181 ttgcatttgg atatcagcca ggtcaagcaa aataccagcg cgttttgtta tattgatacg
     9241 tattaacgga tctttatttt tttatgcaaa gtgactatta tccatcattt tgttcgtaag
     9301 gatatgtgca atgttcggtt ttatttcaga tttattaaca gcagcagtca gttctttaga
     9361 tgaattatta caagatactc cggcacatca aatcatatta ggcacagcgg ctctttattt
     9421 tctttataat caataccata acccatcgat ttccaggtgg taccgtagcc gtaataacgc
     9481 cagtatgaag cagcgtatta ttgatagtgc ttacgccttg gcaaaaaatt tacctggcgt
     9541 gaatcagatc attgagaaag agctcaataa agaactctct tcaacgagag aaaaactgag
     9601 aattcagcgt tccggtatga ctctcaggga ggaaatacct gaggaagggc tttctcccca
     9661 agatattttg agtgcatttg atgtggatgt ggaaaaatgt cattttgatt ttttatccgt
     9721 aactaatgac agcactgaga aggagtttct tgttggaaga ggggatggta aagattcagg
     9781 tgcattatac gctattcacc ctaaggagct gacagagctg cttaaagaag tttatggggc
     9841 aaccgctcta actaatcctt tgcatgataa atggcctcga attaacgcaa tgcaagcgga
     9901 ggtgattcgc tggtgtcaaa atttgtttca tggttcaaag gaaggttatg gtttattaac
     9961 tcatgggggt acgaccagca ttattgaggc gatggctgct tatgtcattc gcgcaagggc
    10021 aaaaggcatt gattatcctg aaattgtcgt tcctgaaaca gcacatgctg catttaaaaa
    10081 agcagccgag ttaactggag caatattaat tacggttcct gttgataaaa aaacgggtgc
    10141 ggtgaatccc aatgtcatga gttcctatat taccagaaac actgccgtaa tggttggatc
    10201 tgccccatct ttcatgaatg gaattcatga tcctgtctct gaattaggtc aattagcaaa
    10261 aaaaaagaat gttccttttc atgtagatgc ctgtttgggt ggatttttaa cggctttttt
    10321 agatacctca tcccagccta tggactttcg agtacctgga gtgacttcca tttcagccga
    10381 cttgcataaa tatggttgtt gtccaaaagg cacctctgtt tgtcttttca gtgaggattc
    10441 gcctgctttg tctgtttatg ccgcattaaa ctggtcaggt gggttgtatg ctacgccagg
    10501 aattctggat ggctcaacga gtggtgctcg tgtagcggaa gtctatgcta cattgtctta
    10561 ctatgggaaa aataaatatc aggaaatcgc caaaagcatt atcagactac gtaatgctat
    10621 tcaaaaagaa ttgaaggcat tagtggagga aggcaatgga ttaaccagcg aggatattta
    10681 tgtttatggt aatcctcaat ggtctattct gggttttaga agtaacactt acaatgccca
    10741 tttcattgcc gatgaattgg agaagcgggg gtggaagctt aacttgctac aaaatccgga
    10801 tgggtttcat ttatgtttga cccatgtgca cactttagta agaggcttcg agacgcaatt
    10861 tatcaaagat ttgagagagg cggtgattga tgttaaaaat tatccccctg gaaagaaacc
    10921 aagcggtaat gtcaaagtgt atggtgctgt cggtatgatg cctattgagc ttcagaaaga
    10981 aatttgtaaa caatatcaaa aagcccgcct tgattttaca gcagccaacc acgggagctt
    11041 ggggatattt acaccttcat ctacagaaga ggacgatgga cttagaaaca ggaaagtagg
    11101 ggaacaaaaa gtgcaaacct cgctttgaac caatagagct cttgcataac cagtctttac
    11161 ccttttattg ctgccttgca aacgtttctg cggtgctgat ttactgcatc taaactacgc
    11221 tcgctgagac gtttgcgcct tgccgtaaaa caaaataatc agcttatgta agaggttcgc
    11281 ttttactata aaaattacac gcgtttctgt acagaatttt ttctgtcagc tcctcatcaa
    11341 atagatcccg gattaattga atgtcactat ccagaaaagg gcggggagcc cgcgtggagg
    11401 gtaaatctgt accaaataac aaagcgtctg gattaatttg ggcaatggtt tgcagtgctt
    11461 ttttaacatc aaaatcgact ctcccaaaac ctgaagcttt tattttgatg ccatgctcca
    11521 ccagatggag taactcggaa aatcctgact gtgataatcc taaatgatca atactgacag
    11581 ctggcaattc caataacaga ttagtcaaat caccaagctc ctttgagtct acataaatct
    11641 ctacatgcca ccttaccatt tcataaatac gatgagcaaa atactttaac tgatggatat
    11701 tttctgagcc tccacgcttg agattgaaac gaaccgctcg aatgccttgt tgatttaatt
    11761 gaataatttc ttcatcactt acagtggcag gtaattgagt aacgcccaca aagtttgggc
    11821 ctaaaaccga taaagccgtt agcagatagg tttggtcaaa ggcttggaat gaacctgaaa
    11881 ccacggctcc tccacaaata ttcaggggct ttgcacgttg gagataatct tcaacagtaa
    11941 attcgggagg aagataggat tgattggcga ctagaggaaa acgataatca ataatatgaa
    12001 aatgagcatc aaatagtttc agcatttcag tcaattaaat ttgcgattaa aatcatacta
    12061 tcacacaaag gtttcattcg ttattagcga ttggtttttt gcttgatgac tataaatgat
    12121 ataaggccat tctctaattt atttgtcagt tgacagatag aatacttgtt gctattaaat
    12181 tgtaagtggt ttttataata tgggccgtta gctcagttgg tagagcagga gactcttaat
    12241 ctcagggtca taggttcgaa tcctatacgg cccaatcaat tattaatccc ccaatcttca
    12301 caaattaaaa tatgctttat taattcgtgt tattgctaat gagttttatt atagtattgt
    12361 cattctttct catactcata tggtataaac aatgcaccag gagttattaa agtatttaga
    12421 aatgtatcac ccgcaaattg cgccatttat gcatcttcag attaatgaat ggggcaaaaa
    12481 cagaccactg ggaggggtga aaatactgca tcatgtgcct ttggttccca atacactgct
    12541 aaaaatagca gctcttgtcg ctgcaaatgc tgaggtgacc gtaaccaatc cggcttcttt
    12601 ttgtcaggca catcctgatg cgattacttg tcttcataaa tcaggaattc cttatgttga
    12661 aaatcttgat cttttaagag atgaaacgtt cgatgtttat ctggattgtg gtgggcaact
    12721 tcatcagagt ctcggaagac ctttgagagg ggctattgaa ctaacgggct caggggatca
    12781 gtattatcgt agtctgaaaa cacttgattt tccggttata agcattgatc acacaatgac
    12841 gaaacaattg gaaacagtat ttggctgtgc tgaaagttct caccaagcga ttcaaaaact
    12901 cactggtgtc gatcccacaa acaagaattg gctaatcttt gggtttggta aaatagggcg
    12961 agggctcgct tatttttgtc tggaacatca agtaccagtt actgtagtgg atagttcagt
    13021 ccatcaatgt gatttagctt ctaatcttgg tatagaggct attagacccg aggagcatga
    13081 taagcttgaa aaagcagtgg ctaaagcaga tattatttta actgcaacgg gcggtaaaaa
    13141 tatcatgagc aaatacccca gatcctggtt tgatggcaaa atcttgggga atttagggct
    13201 gcatgatgag tttggtccca atttcacctc tcaggatgta ttatacgata aaaaacctat
    13261 taatttttgc ctatatgatc ctacgccaat gatttttata gatcctgagt tttatttgca
    13321 taatctggga tgtttattgc ttttacaggg aaattttcct cggggaatgc atgatattcc
    13381 tgaagatata gattcctcta ttattaaaga ctggtgccaa tatcataggc gttctgaaaa
    13441 aagtattcga cgttggtttc tgacttc
//
LOCUS       DASLOE010000078        13263 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.078, whole genome shotgun sequence.
ACCESSION   DASLOE010000078 DASLOE010000000
VERSION     DASLOE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13263
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.078"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            397..1911
                     /locus_tag="U6309_001734"
     CDS             397..1911
                     /locus_tag="U6309_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN5505174.1"
                     /translation="MSYMKIEELLLKDTMRRAQIPGVSIAYVNHQGVISTQEIGLTDG
                     FGLVKMSNDPTRCPFQELVLGSKSELGVGTKNTVITFNKELYYVDQAEKIVRKIPLTE
                     ANKIVYEMLHAKCTETYQLADSNEHELIAALTGHMPPTEVKPETVFGAASLSKPVFAY
                     LVQKLIQTNAKNQSEAGFDQFILPEKLKHFDLDTPLFHIIPLEEFNIDGMKFDLSDES
                     IVDYSKVLTTRMVLSHTTGLAHGEMKFQFLPNSEEKEHGYSNVGIIYLQQVIEKLTGS
                     DLETLAQKHVFEPCGMIHSTYGLKACAANSLRTTACDYANFVKHLIHDSTIENPFVPN
                     AYMTKDKGQAGAIGIAKGNIPDTILQHVAWGLGWGLQTNDEGRVITAYHSGDMNDYRA
                     WVVIDLQDKDKKNAVVFFANSHNGHILAEQIIPKTMQMEQAANYFFSKWGFARNLAEL
                     GGKTTRLGIKSSSGSSDSSPDTSIQDEPVEIKKETEKSDTSSIETYTSGWTFNA"
     gene            complement(1888..2808)
                     /locus_tag="U6309_001735"
     CDS             complement(1888..2808)
                     /locus_tag="U6309_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN5505175.1"
                     /translation="MAIPFKRINLNLILHLDALLSEKSVSAAAEKVFVSQPAMSSSLK
                     QLRELFKDPLLIPGKGEYFLSPKAKELQPQLEQVMFFIRNILQPNERFDPFQTKRSFK
                     IALPDYVELILLPSLMAALEKLPHIVIETTIQSMADDPHLFIDENVDLAIGAINENSL
                     SAQLKVEKLYEEEIICFASKNNPLINRPLSLEDYISNKHIAFSFNQERYSHSAEYLYK
                     RKLVRNDMLFVKNILPAIFAVANSDAVISTAPSFLINMFARQFNLGMQALPFNLEPTP
                     IYLVTHTKSQYDEGVSWLAKLISSSIKSPS"
     gene            complement(2992..4506)
                     /locus_tag="U6309_001736"
     CDS             complement(2992..4506)
                     /locus_tag="U6309_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEN5505176.1"
                     /translation="MFGELFASSIVLGVRYYSNQAYQAVAGLLGQIERFGIGYTREFI
                     RDPKSMIQSLTSKARSSEAGYSVVSLGYLGGNYAVITPKSQSQLTELLNQFDQEKSGR
                     RGHASFKFFSGNDFFIISDTGHAALSSRKNLSRFLVPSRLVNPTITEVEKRVDCDVDI
                     QLRHTICGIVRAVMVENMLGIKQLPDNTYDVMASYRNDVKRWGAFPFPELLNFMPSLR
                     QKRDVYRAFSKGILEQEFEKLVEVLHDDPNPGNTNLIAASVVSLFKDENPTLSNEELL
                     SALRSLSIEDIRKYFENPIVQSLPMILKAADNLTDAIVLCLEQIASDPDKFAMLRDEM
                     DRSHLAIEEGIDVRALKTLPVLDAFYKESVRFDAPVAVPRYTQNGYSSDSMTIPPNTM
                     MIFDLHALAKGEQYWTNPEEFDPTRFLQQEKEQVTEERGMNSNNKNHTVGQFPFIPFS
                     VGLRNCPAFAVTESLFKVTIAKFVSDYQLQLVKKDEEDSTLHITSRDKSFGLMQ"
     gene            4804..5718
                     /locus_tag="U6309_001737"
     CDS             4804..5718
                     /locus_tag="U6309_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505177.1"
                     /translation="MATTLIQTPSYTKNLTLNLDDYPGGVAIWGALPALFDTSNQGFD
                     RGIHVHARLADSSKKVIDATYDHVTIISGYRIFTITEEAAVHFSMSAIFDIKITSLTC
                     QHCSQLITSVGYAAVRPSRQHQCNHCSEITTTTSECISNPIMLLKELIGDEQVKRPAV
                     IPNRTIAIDPDKYSGGIQIWGSNPSIIWTAKRLEESAIHIHAYNENGKRIIDNTYGSV
                     SLDGHKLDIEMIRVLQIQLALPNLALLLTTVYCPHCGVEQFDRGIWAVSAHNHRICLL
                     CKQTFISQDVISNPAFDVLTHVSGVISQ"
     gene            5715..6116
                     /locus_tag="U6309_001738"
     CDS             5715..6116
                     /locus_tag="U6309_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN5505178.1"
                     /translation="MSTLKTIFKIGALIFNEKNQLLAVHKKGKPPMELIVPGGVMEEN
                     ESDEETLRREIKEELDSDIISFQFYKQFEDKAIYEEKWLVMRTYIVTLGNTPKPANEI
                     DQLVWLGHDYHQSGYRFGSILGKQIIPDFFK"
     gene            complement(6200..7354)
                     /locus_tag="U6309_001739"
     CDS             complement(6200..7354)
                     /locus_tag="U6309_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505179.1"
                     /translation="MKVLFDKTIQNNPGSGITPSSKSNEICTSSICMKSKLPADVVAK
                     HLHGLQSTMVDRRNPDLSDSKSLMKSAKAHAILKTGTEIDMLTATGISEDDLPSSKGA
                     IPMTAPEELTMKATWKNKNQERGISGRDASVFGGLGVLVISPDAKVMNIPKMNALAVP
                     ASFLAKYGAELIPLDQPFTLDTVDKSSLENLYLVEYQWDADYIQDYVMNIRGGGGLFV
                     ETHPFPHVFTPLSPACRGALILGVERKDGAFDFAAFEIPFGYTMKIASNVIHGDSFFV
                     GPYAIALTETELADSVLLKQDTPQRDIQRVTQVPTKVFTIFLIEETKLASQINRKMMA
                     DKICHEGLSGKRLGFFKESSPKASTEVRHCSDAEQDIHDQQFGLVPCLTK"
     gene            7688..8785
                     /gene="lem23"
                     /locus_tag="U6309_001740"
     CDS             7688..8785
                     /gene="lem23"
                     /locus_tag="U6309_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem23"
                     /protein_id="HEN5505180.1"
                     /translation="MKRKDEQNNLTKGKANEQNTVSTNTHLLFSKPPGINADTANHMA
                     SSIDRLKDFDSSKQCLWLKYSEEIINEFCALLPEFPKKKPKVREDIRCPEEALLTQVG
                     CAYAFLASKRREVAKILKHGPLRHQFGLFNKSGFASIDDFNLSKDIPSQKDKIYEHLS
                     LLISQPNPFIEVKKNHREAEGFSIYTITFKMKPDEIEQVHKKNLDSNKPFIENDTVLT
                     ITFNDYGKNDNRTSIVIDHCPTPFAENYTSESSYFKVQFELIEPYFQACQAWEASDGI
                     DQFLENAGRIAYILARMQPVGRGNSAIVEWMIRGLAQTKNIELGPFNPDEKIGWDFKA
                     FLTPEIQDYAHWFSEKAFAYSQLKNVTSLGH"
     gene            complement(8967..9272)
                     /locus_tag="U6309_001741"
     CDS             complement(8967..9272)
                     /locus_tag="U6309_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2407 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505181.1"
                     /translation="MYNVVSFNNEGVEHMKPTNEKSKEIRKKYKAPGASDEQLANSIK
                     VASEFEYKNSSTFGWGGVYMVDRFQSMITGRAITNNTQRLLDAQALEEEASKANLKQ"
     gene            9496..10155
                     /locus_tag="U6309_001742"
     CDS             9496..10155
                     /locus_tag="U6309_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505182.1"
                     /translation="MLTKNLNEALSAIKYVKSKLVYGSNTEKDSKKLNWEPGRIILLR
                     KEVHALAVVYARSHNGKAGFDVISKLTHAALLKYGFGNCGEQAQTAFCYLQSRGVAPL
                     DYCQTSIGEHCLIVIGRVSGSDPNDISTWGEDAVICDPWAEKAYPLSEFRAMQSPDND
                     IRYPEICYANGKSPKPYLAGELISHHRIEPASKASSLSFFKKESYEHSQLNSATPMLI
                     A"
     gene            10480..11298
                     /gene="ceg29"
                     /locus_tag="U6309_001743"
     CDS             10480..11298
                     /gene="ceg29"
                     /locus_tag="U6309_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector Ceg29"
                     /protein_id="HEN5505183.1"
                     /translation="MTNSNHLRNPVTKSLNDLCQDELSVIGSYLGAWNDLWSLTFTSS
                     EMKKCLIHQQTDELWKFFFKEFFPYIPLPTEDFYKNFMKNYKIWYQTLYRVKQDPFFL
                     MYLDKLKEDPAHLFRNDKEIIIAAIEKQPFLYKNFSRGYKEDEEVILCALKNNRFIIH
                     DVPRTVREGFKEIADIKEDKARVNACDLLIEKLSPQRVSKIKSLDLFKSQLTLQAQNI
                     TKKSGEQVNNRQLQRSTAPITPEGITSLVHLGLFRDTQIQYEEKEESLHNKYRK"
     gene            complement(11419..13116)
                     /locus_tag="U6309_001744"
     CDS             complement(11419..13116)
                     /locus_tag="U6309_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEN5505184.1"
                     /translation="MIAKSKTDMNRIQMGTNKEFPGKSHLKPSKSCGTDGSNFQVEFL
                     LDVIQKLSLAKDLETIMFIVRKAARTLTGADGSTFVLRDGNQCYYAEEDAIAPLWKGK
                     RFPMSTCISGWVMEHRQSVIIEDIYSDPRIPFDAYRPTFVKSLAMVPIRTAAPLGAIG
                     IYWAEYYFPTEEQLNWLQVLADSTSVAMENVMLQSELEKGQKETTAQIEMTRKLMEVN
                     KNLECALYELNHRNKEMQWLKELSSTLQTCLYIKEAYHLIALYAAKLLPESTGVFYVM
                     HPSRNYLEAMSSWGDSLIEERIIKPDDCLGLRRGSIYTVEEPQKDLICPHHTINSNLK
                     PYTCIPLFAQSDILGLVCMEWKSFKKEAEEEINKENNQEILASMMAEQIALGISNIML
                     RETLRNQSLRDVLTGLYNRRYLNEALDRELNRCARKSLSLAVLMLDIDHFKQFNDKFG
                     HEAGDMVIQAFANVLRQVTRKEDIARRYGGEEFILIIPEIDLEAALQRAQSIHDAVSR
                     IHLRYGGNALTQITISIGLTMYPAHGNDMQDLITAADNALYEAKNSGRNKTVLYHKN"
BASE COUNT         4276 a         2616 c         2405 g         3966 t
ORIGIN      
        1 ctgaaatacc cttttaagac ttaagtgggc taacctctca agtttttctt cgtgtacttt
       61 caatttccaa ccttattttt caataaagca aattcaattt aactcagcat ctataagaaa
      121 aatgtataca aaatacacaa aaattgattt taatcgcaat cagttaatat ttattgttca
      181 ggagaatttc ataatgaact acaagctaat attagccaca ggcagcatcc tgacagaaga
      241 gctctcttct atccctcgct atattgtacc aatccagcaa aaaccggggt attagcgggt
      301 ttttacgata tttttgcgaa aaaaattttt gagtaaagcg aacacgttaa tacccaaagt
      361 ataatttatg gccaaattat ttttttgagt gaatttatgt cgtacatgaa aatagaagaa
      421 cttcttctaa aagataccat gagaagagcc cagatccctg gggtatccat tgcttatgta
      481 aatcatcaag gagttatttc cacacaagaa ataggactta cagatggatt cggccttgtt
      541 aaaatgtcaa atgatcccac ccggtgccca tttcaagagt tggttctcgg atctaaaagt
      601 gaattaggtg tgggaactaa aaacacagtc atcacattta acaaagagct ttattatgtc
      661 gaccaggcag aaaaaattgt ccggaaaatc ccgctgacag aggcaaataa aatagtctat
      721 gaaatgttgc atgcaaaatg caccgaaacc tatcaattag ccgatagcaa tgaacacgaa
      781 ctcattgcag ctctaacagg tcatatgcca ccaactgaag tgaagcctga aaccgttttt
      841 ggcgcagcgt ccctaagcaa accggtattt gcttatttag tacaaaaatt gatacaaacc
      901 aatgcaaaaa atcaatctga agcaggtttt gatcaattta ttttacctga aaaattaaag
      961 cattttgatt tggatactcc ccttttccat attatccccc tggaagaatt caatattgac
     1021 ggaatgaaat ttgacctgtc tgatgaatct atcgttgatt attccaaagt actaaccacc
     1081 aggatggtgt tatcgcatac cactggactg gctcatgggg aaatgaaatt tcaattcctg
     1141 cccaattctg aagaaaagga acatgggtat tcgaacgttg gaattattta tttacaacaa
     1201 gtaattgaaa aactgactgg ctcagatctt gaaacactgg cccaaaaaca tgtatttgaa
     1261 ccatgcggta tgattcatag cacttatggg ctcaaggcat gcgcagccaa ttctctacgc
     1321 actacagcat gtgattatgc caatttcgtc aagcatttaa tccatgacag cacgattgag
     1381 aatccctttg ttccaaacgc ttacatgaca aaagacaagg gacaggcagg agcaatcgga
     1441 atagccaagg gcaatatacc cgatactatt ctgcaacatg ttgcatgggg cttaggctgg
     1501 ggactacaaa ccaatgatga aggaagagtt atcacagcct atcattcagg cgatatgaat
     1561 gattacagag cgtgggtagt gatcgattta caggataagg ataagaaaaa tgccgtggta
     1621 tttttcgcta actcgcataa tggacatatt ctcgctgaac aaattattcc taagaccatg
     1681 caaatggaac aggctgccaa ttactttttt tcaaaatggg gttttgccag aaacctcgct
     1741 gaattaggcg gaaaaactac tcgactgggt attaaatcat cctcaggtag ctctgatagc
     1801 tctcctgata cttccattca ggatgagccg gttgagataa agaaggagac agaaaaatca
     1861 gatacttcct caatcgagac ctatacttca ggatggactt ttaatgcttg aactgatcag
     1921 tttggctaac caggagactc cctcatcgta ctgactcttg gtatgggtca ccagataaat
     1981 aggagttggt tccagattga aaggtaaagc ttgcattccc agattaaact gtctggcaaa
     2041 catatttatc aaaaaactcg gggctgtgga aatcaccgca tcggaattgg ctactgcaaa
     2101 aattgcaggc aagatatttt ttacaaaaag catgtcatta cgtaccagct ttcttttata
     2161 taaatattca gcggagtggc tataacgctc ctgattaaaa ctaaatgcga tatgtttatt
     2221 gcttatgtaa tcttccaaag acaatggcct gtttatgagt ggattgtttt tgcttgcaaa
     2281 acaaataatt tcctcctcat acagtttttc aactttgagt tgcgcggata gagaattttc
     2341 attaatagct cctatggcca ggtctacatt ctcatcgata aatagatgtg gatcatcggc
     2401 cattgactga atcgttgttt caataacaat atgaggcagt ttttcaagcg ctgccatcag
     2461 ggatggcaac aaaattaatt caacgtaatc aggtaaagcg attttaaatg atcttttggt
     2521 ttgaaaggga tcaaatcgct cattcggttg cagaatattt cttatgaaaa acatcacttg
     2581 ttctaattgt ggctgtaact ctttggcttt tggagagaga aaatattccc ctttaccagg
     2641 aattaatagc gggtctttaa atagctctct cagttgtttt aaagaagaac tcattgccgg
     2701 ttggctgaca aacactttct ctgcagcagc actgacgctt ttctcgctta gtaaagcatc
     2761 cagatgtagt atgagattta aattaatacg tttaaaggga atcgccattc gatcctctcg
     2821 gcaattgttc tcttcttata ttagtataag aaatttaaaa gtatccaata tgaataattc
     2881 attatcaaga cagggtgtat tttgagaggg gttacaatac cacttaccga accaggtaac
     2941 gctgtcaaat agacctcatt acctggtatc aatctattgt tctttccagt gttattgcat
     3001 tagtccaaaa gatttatctc tggaagtaat gtgtaatgtt gaatcctcct catctttttt
     3061 aaccaattga agttggtagt ctgaaacaaa cttggcaatc gttactttaa ataaggattc
     3121 ggtcactgca aatgccgggc aattgcgcag tcctactgaa aagggaataa aggggaactg
     3181 acctacggta tgatttttat tgttactgtt catacccctc tcttcagtaa cttgttcttt
     3241 ctcctgctgt aagaaacggg tagggtcaaa ctcttcagga ttagtccagt attgttcacc
     3301 tttagctaaa gcatgcaaat caaatatcat catggtattg ggtggtatgg tcattgaatc
     3361 ggatgaatag ccattttgag tatatcttgg cactgcaacg ggtgcatcaa atcgcactga
     3421 ttccttataa aaggcatcta atacaggtaa agttttaaga gcccttacat ctatcccttc
     3481 ttctatggcc aaatgcgatc tgtccatttc atcacgaagc attgcaaatt tatccggatc
     3541 tgatgcgatt tgctccagac aaagaacaat tgcgtcagtg agattgtctg ctgcttttaa
     3601 tatcatcgga agcgattgca ctataggatt ttcaaaatat tttctaatgt cttcaatgga
     3661 taaagacctt aaggcagaga gcagttcttc attggaaaga gtcgggtttt catctttaaa
     3721 taaactaacc acagaagctg caattaaatt agtgtttcct ggattaggat catcatgaag
     3781 tacttcaacc agtttttcaa attcttgctc tagaatacct tttgaaaaag ctcgataaac
     3841 atcccgtttt tgtctcaaac tcggcataaa attgagtaac tctggaaaag gaaatgcccc
     3901 ccatcgcttg acatcatttc gataagatgc catcacatcg taggtattgt ctggtaattg
     3961 cttgatgccc agcatatttt ccaccatcac tgcgcgaaca attccacaga ttgtatgtcg
     4021 aagctggata tccacatcac aatcgacacg tttttctact tctgtaatag tgggattaac
     4081 cagccttgag ggcacaagaa aacgggacag gttcttccgc gatgacagag cagcatgtcc
     4141 agtatctgaa ataataaaaa aatcattacc agaaaaaaat ttaaacgaag catgccctct
     4201 tcggccagat ttctcctggt caaattgatt caataattca gtcagttgag attgagactt
     4261 tggagtaatg accgcgtagt ttccccccaa atatcccaaa ctgacaacac tatatcctgc
     4321 ttcactgctt cgtgctttgg aagttaatga ctggatcatc gatttaggat cacgaataaa
     4381 ttctctagta tagccaatac caaatcgttc tatttgcccc aaaagacctg ccaccgcctg
     4441 ataagcctga ttggaataat aacgaacacc caacacgatt gaacttgcaa ataattcacc
     4501 aaacataaat catcccaaac aataggccaa aaaattggac tatagtaata catttagaaa
     4561 atattgaaca cacccaggcg tttttttatt tcatgtgggt tgattatatg atgcttatta
     4621 ttcacccctt tcaaatacca ggaaatgatt ccgacaccga tacttgtcaa gaattagcag
     4681 gagcgataca atgactttgc ctaaaaagtt atggtcttca gcaagtctcg attcctccgg
     4741 tgctgggtat aaagacataa ttttggtcga gggatacttt cgttttttta tacgaggtat
     4801 cctatggcga cgacattaat tcaaacaccc tcttatacta aaaatctaac tctcaacctt
     4861 gatgactacc cgggaggggt tgcaatttgg ggggcattac ccgctctttt tgatacttca
     4921 aaccagggat ttgatcgagg catccatgtt cacgcgcgtc ttgccgattc atctaaaaaa
     4981 gtgatagacg caacctatga ccatgtcaca attatttccg gatatcgtat ttttacaatt
     5041 acagaggaag ctgctgttca tttcagcatg tccgccattt ttgatatcaa aattacatca
     5101 ttaacatgtc agcactgctc tcagttgatt acttccgttg gttacgctgc agtcaggcca
     5161 tcacgccagc atcaatgtaa tcattgtagt gaaatcacga ctacaacttc agagtgcatc
     5221 agcaatccaa ttatgttact taaagaattg ataggagatg agcaagttaa aaggcctgcc
     5281 gttatcccca atcgtaccat tgcaattgat cctgataaat acagtggtgg tattcaaatt
     5341 tggggctcta atccatcaat tatttggact gctaaaagac tggaagaaag cgctatccat
     5401 attcatgctt acaatgaaaa tggcaaaaga attatagata atacttatgg aagtgtttct
     5461 ttagatggtc acaaactgga tattgaaatg attagagttc tacaaatcca attggcattg
     5521 ccaaatttgg cattactcct gacaaccgtg tattgtcctc attgtggtgt ggaacagttt
     5581 gatcggggga tttgggctgt ttctgcgcat aatcacagga tttgcttact atgtaagcaa
     5641 acttttatat cccaagacgt gatcagtaac cctgcttttg acgtactaac ccatgtttcc
     5701 ggagtgatct ctcaatgagc acattaaaga ctatatttaa aataggcgcg cttattttta
     5761 atgaaaaaaa tcagttactt gctgtacata aaaaaggaaa accacccatg gagctcattg
     5821 ttccaggtgg tgttatggag gaaaatgaaa gcgatgaaga gaccctgcgt cgagaaatca
     5881 aagaagaact tgactccgat atcatatcat ttcagtttta taagcaattt gaagacaagg
     5941 caatctatga ggaaaaatgg ctggtaatgc gtacttacat tgtcactctc ggcaataccc
     6001 ctaaacctgc taatgagatt gatcaactgg tctggcttgg gcatgattat catcaaagtg
     6061 gctatcgttt tggatccata ttaggcaaac agattattcc tgatttcttt aaataaacca
     6121 ttcccagaca ttcacagcaa cttttggttc agaccaggaa gtaaagtccg aacctttatg
     6181 agatgaggat aaaatgatgc tatttggtta aacaaggcac taatccgaat tgttgatcat
     6241 gaatatcctg ctcagcatct gaacaatgcc gaacttcagt acttgccttt ggagatgact
     6301 ctttaaaaaa acccaacctt tttccggaaa gaccttcatg acagatttta tccgccatca
     6361 ttttacgatt gatctgactg gctaatttcg tttcctcaat taaaaaaatt gtaaacactt
     6421 tggtaggaac ttgagttact ctttgtatgt cacgctgagg tgtatcttgc ttcaaaagaa
     6481 cagagtcagc caattctgtc tcagttaatg caatggcata agggcctaca aaaaaagagt
     6541 cgccatggat tacattactg gcaattttca tggtatagcc aaatggtatt tcaaacgcag
     6601 cgaaatcaaa tgccccatct ttgcgctcga ctcccagaat caatgcacca cgacatgcag
     6661 gggacaaggg cgtaaaaaca tgaggaaatg ggtgtgtttc cacgaacaat ccaccaccac
     6721 ctcttatatt cattacgtaa tcttgaatat aatcagcatc ccattgatac tcaaccagat
     6781 acaggttttc cagtgacgat ttatcaacag tatccagggt aaatggctga tctaggggaa
     6841 tcagctcggc accatatttg gctaaaaatg atgcaggtac agccaatgca ttcattttgg
     6901 ggatattcat tactttcgca tcaggagaga taaccaatac tcccaagcca ccaaaaacgc
     6961 tggcatccct cccactgatt cctctttcct ggtttttatt tttccaggtt gctttcatgg
     7021 ttaattcttc cggcgcagtc atgggaatag caccttttga agaaggtaaa tcatcttcac
     7081 ttattcctgt agcagttaac atatcgattt ctgtgcctgt tttaagaatg gcatgagcct
     7141 ttgccgactt cattagggac ttactatcag acagatcggg atttcgtcgg tcaaccatcg
     7201 tggattgcaa accatgtaaa tgctttgcta ccacatcggc tggcaatttg cttttcatgc
     7261 atatggaaga agtgcatatt tcattacttt tactggaagg agttattccg gatcccggat
     7321 tgttttggat agttttatca aagagtactt tcataattat aaccacacat ctttatagat
     7381 taaaatatga cgcaattgat ctattaaaat agataaaggt taaattatga actaaaaata
     7441 ttaagatttt attaaattaa gaactatatg ctcaaatata atcaataatt ttgttctaat
     7501 atgatattaa aaatagagtt gggagactgc atatatttca ttgcatgatc aaataatagg
     7561 aactaaatac caaattaagg ttggtaaaat ttaaaaatac tgttgcttat taatactcat
     7621 ccagcatatc acatcccgaa attatgcttg ctatacttaa tagtacacaa caagttctac
     7681 gggtattatg aaaagaaaag atgagcaaaa taaccttact aaaggcaagg cgaatgaaca
     7741 aaatactgtc agcaccaata cccatttgtt attttcaaaa ccacctggta ttaatgccga
     7801 tactgcaaat catatggctt catctattga ccgcttaaaa gattttgaca gctcaaaaca
     7861 atgcctttgg ttaaaataca gtgaagaaat catcaacgaa ttctgtgctt tattacctga
     7921 gtttcccaag aaaaaaccca aagtacgtga agacattcgc tgcccggaag aggcattatt
     7981 aacccaggtc ggttgtgcct atgccttttt agccagtaaa agacgcgagg tagccaagat
     8041 tttaaaacat gggcctctaa gacatcaatt tggtttattt aataagtcag ggtttgcctc
     8101 aatcgatgat ttcaatctca gtaaagatat cccttctcaa aaggataaaa tttatgagca
     8161 cctctccttg ttaattagtc aacctaatcc ttttattgaa gttaagaaaa accatagaga
     8221 agcagaaggt ttttctattt acaccataac attcaagatg aagcctgatg aaatagaaca
     8281 agtgcacaaa aaaaatttgg attcaaacaa accatttatt gaaaatgaca cggtactgac
     8341 gataaccttc aatgattatg ggaaaaatga caacagaacc tcaattgtca ttgatcactg
     8401 ccctactcct tttgctgaaa actatacgag cgaatccagc tatttcaaag tgcaatttga
     8461 attgattgag ccttactttc aagcatgcca ggcctgggaa gcttctgatg gaatcgatca
     8521 gtttttagaa aatgccggaa gaattgctta tatactggct cgtatgcaac ctgttggacg
     8581 tggtaattct gcgattgttg aatggatgat tagaggcctt gcccaaacca aaaatatcga
     8641 attaggacca ttcaaccctg atgaaaaaat tgggtgggat tttaaagcgt ttctgacgcc
     8701 tgaaattcag gattatgctc attggttttc cgagaaagcc tttgcgtata gccaattaaa
     8761 aaatgttact tccctgggac attgaattaa gctgagtaaa ccagcctatg tggtaaaaaa
     8821 atttatagaa ttgtaataaa tagattaaag ataatcccct aggatataac tgcaggtaag
     8881 aagataaaag agaacgccgg gattcaagtc ttgcttttga ctataagaca caagaaaaat
     8941 gaaagacttg ccccaagata aaagcgttat tgttttaagt ttgctttaga agcttcttct
     9001 tcaagtgctt gagcgtccaa taacctttgt gtgttgttag tgattgcacg acctgtgatc
     9061 attgattgaa agcgatcaac catatataca ccaccccaac cgaaagtact gctgttttta
     9121 tactcgaact cagaagctac cttgatagaa ttagccagtt gctcatcaga agcgcctggc
     9181 gctttatatt ttttacgaat ttctttactt ttttcattgg ttggtttcat gtgctcaaca
     9241 ccttcattgt taaaactgac gacattatac atgaataata ttcaaacaca atttatttaa
     9301 aaaaaacaat ttgatctaaa tttttcacaa atatttcttt agtaaaaaac tctccattac
     9361 atggccttta caatcacttg agtttccaaa aaataaaatc tcacaatcta aaaacaaacc
     9421 caaatagata aaatattaac aaactgtatt ttatttgata taataagtct gttttaccct
     9481 ctagagattt ttttaatgct caccaaaaat ctgaatgaag ccttaagcgc aataaaatat
     9541 gtaaaatcca aactagttta tggttccaat accgagaaag attcaaagaa gctaaattgg
     9601 gagccaggaa gaattattct cttaaggaag gaagttcatg cactagcagt cgtctatgct
     9661 cgttcacata atggcaaagc aggatttgac gtcatctcca aattaaccca tgcggcactc
     9721 ttaaaatacg gattcggcaa ttgcggtgag caagcacaaa cagcattttg ttatctgcaa
     9781 tctcgaggag ttgcgccgct tgattattgt caaacatcaa taggagagca ctgcctgata
     9841 gtgattgggc gcgttagtgg ttctgatccc aatgatattt cgacatgggg agaggatgct
     9901 gtaatttgcg atccctgggc tgaaaaagcc tatcctcttt ctgaatttag agcaatgcaa
     9961 agtcccgata atgatattcg ctatcccgag atatgttatg ctaatggtaa aagtccaaag
    10021 ccttatttgg caggggaatt aatcagtcat cacagaatag agcctgcctc gaaagcatca
    10081 tcgctctctt tcttcaaaaa agaaagttac gaacattcac aattaaatag tgctaccccc
    10141 atgcttattg cttaaagcag tccagtgcca aagtgcaatg actaccatgc gtaccaattg
    10201 ctaccttttg ttgtaacgaa tcatcaaatt atcctgacgc tattgattaa aatctggcaa
    10261 agagaaaaaa cacaccttgt gcgagcaaag aaaagccagg atctctgatg ctctcttttg
    10321 caaaataacg ccaaaatcat ccagtaagaa aaattaatct atctattgtt aactattaat
    10381 attctcttaa taaaaagaaa gcattatagt catctggaag tcacatttgc atatcaaaaa
    10441 acataataga aaaacaaaac cacaatcagg aggtaaaaaa tgacaaacag taaccattta
    10501 aggaatcctg ttaccaaatc attaaatgac ctttgccaag atgagctgtc tgtaattggc
    10561 tcctaccttg gtgcctggaa tgatttatgg agcttgacgt ttacatcatc agaaatgaaa
    10621 aaatgcttaa tccatcagca aaccgatgag ttgtggaaat tttttttcaa ggaatttttc
    10681 ccttatattc ctctaccaac agaggatttt tataaaaact tcatgaaaaa ctataaaatt
    10741 tggtatcaaa cattgtaccg tgtgaaacag gatccttttt ttctaatgta tctggataaa
    10801 ctcaaagaag atcctgcaca cttatttagg aatgataaag aaattattat agcagccatt
    10861 gagaaacaac cctttttgta taaaaatttt tcccgaggat acaaagaaga cgaagaggtt
    10921 attctgtgtg ctcttaaaaa taatcgattc ataattcatg atgtaccaag aacagtgaga
    10981 gaaggattca aagaaatagc tgatatcaaa gaagataaag caagagttaa cgcgtgtgac
    11041 ctgctaattg aaaagctttc accacaacgt gtttctaaaa tcaaaagcct cgacttgttt
    11101 aaaagccaat taactctgca agcacaaaat ataacaaaaa aaagtggtga acaagtaaat
    11161 aaccgtcagc ttcaaagatc aactgcgcct ataacacccg aaggtatcac ctctcttgtt
    11221 catttaggcc tgtttagaga cacacaaatc caatatgagg aaaaggaaga atctctacac
    11281 aacaaatatc gaaaatgaag cgaataaatg ttgataaggg ttgctctatc gcaaattcat
    11341 tacagtaaca gccaagcata gccttaatag attgaataag gccgggagct aatatgtttt
    11401 gattgcaaat aaaataccct aatttttatg atacaacacc gttttattgc gtcctgagtt
    11461 tttggcttca taaagcgcat tatcggcggc ggtaataagg tcttgcatat cgttgccgtg
    11521 cgcagggtac atagttaatc ctatagaaat tgtgatttga gttaaagcat tacccccata
    11581 acgcaaatga atacgactga ctgcatcatg aatagattga gccctttgta aggctgcttc
    11641 cagatcaatc tctggtataa tcaggataaa ttcttcccct ccatagcggc gggctatatc
    11701 ctcctttcgg gtaacctgac gcaaaacatt agcaaaagcc tgtatcacca tatcacccgc
    11761 ttcatgtccg aatttatcat taaactgttt aaaatgatca atatccaaca ttaacacagc
    11821 aagtgataat gattttcttg cacagcggtt taattctcga tccagcgctt cattcaaata
    11881 acgtcgatta taaagccctg tgagaacatc tcgtaaagat tggttacgca aagtttcacg
    11941 cagcataata ttggaaatac caagcgctat ttgctctgcc atcatacttg cgagaatttc
    12001 ctgattattt tctttattga tttcttcctc tgcttccttt ttaaaagatt tccattccat
    12061 acaaaccaaa ccgagaatat cgctctgagc aaataaagga atacaggtat aaggctttaa
    12121 attactgtta attgtgtgat gggggcatat taagtccttt tgaggctctt ctactgtata
    12181 aatcgagcca cgacgcaaac ccaggcaatc atctggctta atgatccttt cttctatcaa
    12241 agagtctccc catgagctca tagcctcaag ataattacgt gagggatgca tgacatagaa
    12301 aactcctgtt gactcaggta ataatttagc cgcatacaga gcaattaaat gataggcttc
    12361 cttgatatat aaacaggttt gcaatgttga acttaactcc ttcaaccatt gcatttcttt
    12421 attgcggtgg tttaattcat ataaggcaca ttcaagattt ttattgacct ccattaattt
    12481 tctagtcatt tctatttgag cagttgtttc tttctggcct ttctccaatt cggattgcag
    12541 catcacattt tccattgcaa cggatgtgct gtcagccagt acctgcaacc aatttaattg
    12601 ttcctcagta ggaaagtaat attctgccca ataaattcca atggcaccca aaggggcagc
    12661 agttctgata ggaaccatcg ccaagctctt tacaaaagtc ggccgataag catcaaaggg
    12721 aattcttgga tcagaataaa tatcttcaat aatgaccgac tgtcgatgtt ccataaccca
    12781 accgctgata caagtactca tagggaatcg tttgcctttc caaagagggg caattgcatc
    12841 ttcctccgcg taatagcatt ggttgccatc acgcaataca aaggtcgaac cgtcagctcc
    12901 agtcaatgta cgagcggctt tacgcacaat gaacatgatt gtttccagat cttttgcaag
    12961 agaaagtttt tgtatgacat ccaataaaaa ctcaacctga aaattggagc catcagttcc
    13021 acaagatttt gacggtttga ggtgtgattt gcctgggaac tctttatttg ttcccatctg
    13081 tattctattc atgtccgttt tactctttgc tatcatcctt gtctctttgt aattataata
    13141 ctaaatgtgt gttttttaaa caaaatatcc ttcggatcac tcctctctat aagttgtata
    13201 gtaacttaaa ttttcatttc gaataattgt tgaaacaagt tgacgtctgc tcaaagacca
    13261 ttt
//
LOCUS       DASLOE010000079        13242 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.079, whole genome shotgun sequence.
ACCESSION   DASLOE010000079 DASLOE010000000
VERSION     DASLOE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13242
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.079"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(101..1111)
                     /locus_tag="U6309_001745"
     CDS             complement(101..1111)
                     /locus_tag="U6309_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505185.1"
                     /translation="MSTDNLLQPKLIEATLSDYPVIQNMARFYVYDMSRYCGFISEEW
                     ACPPDGLYESYDFKIYFTDPERKAFLIQIENELAGFALLNKFGTQAVDWNMGEFFILA
                     KFQGKGIGQSVAHQLWRTHPGEWEISVIPENIPALSFWRQAVMTYTLNQYKEQIKTVN
                     YDIHQTQRIILNFNTKQQNKSCHSIPQKPITIDFVDSIPESLEKKMTEDLIAYERTHG
                     IDVNYKRFSIVLSAEGIPCGVINAFTAFAEIYIDDIWVDKTYRGKGYGRKLLQTLEDH
                     FKGQGFNNINLCTSAFQAPEFYKKCGYTAEFTRINQANPKLSKTFFVKFFDEENQKQG
                     VF"
     gene            complement(1314..1928)
                     /locus_tag="U6309_001746"
     CDS             complement(1314..1928)
                     /locus_tag="U6309_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505186.1"
                     /translation="MVWDATTTNAISNAVHAFFLLLYLIGACIHYFKKDHTFSLLIVF
                     FFLNLLVLKVLGVYIHYYPSHLHLPPAWIAISLLVIMLNYLLVQSIQMPDLCRVIVVF
                     LSIIFTYLFLTHDGNYTYIALPVVLVCLIAAYYSQAKVRIGFVMVVISNLIWIVTRHI
                     ENYLTGHEIPLEYRYDNDIYHILLILSTYVIYRGIAEGQWRHSH"
     gene            complement(2085..2459)
                     /locus_tag="U6309_001747"
     CDS             complement(2085..2459)
                     /locus_tag="U6309_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN5505187.1"
                     /translation="MLSDPNLVLFYVKNPAKSEEFYKNLLDTQPIESSPTFAMFVMKT
                     GLRLGLWAREEIKPTAHQTGGGMELSFQVSSNEMVDEIYKQWSDKEISIIQPPTQMDF
                     GYTFVGVDPDEHRLRIFCLKRT"
     gene            2649..3422
                     /locus_tag="U6309_001748"
     CDS             2649..3422
                     /locus_tag="U6309_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505188.1"
                     /translation="MNSRIETFHQMERHFFSLISMELLLLEGLKACNSGMPIPALNPV
                     FYSGKSTLLGAEIYQCKRFYESHSTPWALVVPDYLSDTEADKVLAQSGFLSIDQGVAM
                     YLSISDLGISELVSDLLIRRMDNEMDTWILPTIPAFESTEEIAKIYRVRHQEAIEKSP
                     GIYHFSGFIQEQIVCSLTLTVMGDSARIDDVATFPKHQKKGYATQLILSVLQTLKDTN
                     ISWCFLEASADGLNIYKKIGFRELFKNLYYEEQCQYEQN"
     gene            3409..4095
                     /locus_tag="U6309_001749"
     CDS             3409..4095
                     /locus_tag="U6309_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HEN5505189.1"
                     /translation="MSKTERLFHLLQILRRYRYPLSGQFLANELGVSTRTIYRDIAVL
                     QAQGADIEGEPGLGYVLKPGFMLPPLMFSEEEIEALVLGSRWVAKKTDTHLQQAAHNA
                     LAKIASVLPADLRQQLELSSLLIGPGEVISGEDESLALVRKAIRKELKLFIQYHDLKG
                     NVSTRTIWPIGLGYFDQVRVLIAWCELKKDFRHFRADRIVDVENLGVSYAKRRSVLLH
                     DWRKQNNIPG"
     gene            complement(4254..5513)
                     /gene="lem19"
                     /locus_tag="U6309_001750"
     CDS             complement(4254..5513)
                     /gene="lem19"
                     /locus_tag="U6309_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem19"
                     /protein_id="HEN5505190.1"
                     /translation="MIDMISITTYWQQFKDCIDQLRSSVDNKEYSNQIAIADLMIEIG
                     LTIEQLGITQIQQVGDRLHEKAKLFGYRVPFVTPEKKYEFYLPKITNNETLIFAIVYY
                     QHLKNYENVEYQGSINWFRSKVIHGVNEKKISTRAQAEAMLGEIRRYCQSQNIAFNES
                     EFIKKNDESAKEYTTRLFENFHADKTAKSEPIELKQLKESKESNLSDELAKLEKSLED
                     SRNKRNQLIVKIDALAKKLADYHLLSQNHHKLNNEWNNKWFFTKFFDWIMSWFSNDSF
                     IKNLKTSYEQVINAEHELKQEFHPFDTADAYRAELKKQLEETHIECDKNQKQINEYQL
                     KQLEHKLEQRLEQKLQEKLQKDTAVEQKEPSVTKVLSEIKHETNGSDFSSNLNHYYGF
                     FKEHLPNRETLGAIAVGVAAIAIQNLM"
     gene            complement(5644..6156)
                     /locus_tag="U6309_001751"
     CDS             complement(5644..6156)
                     /locus_tag="U6309_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505191.1"
                     /translation="MSVINGKKIITITLGIACLFCSIHPVNSAPDFSNKPHVREKNKN
                     LLGVFVGGNHAQNDEAFTYGFEYHRVLSMPFGFSAVIEHTPVNVEHNNQVELIGLGTL
                     NVFRNLTFGIGPGIRYEKDEPNRMVGRLGIGYIIHFPPDIEITPNIDLDFIEGGEKEL
                     IFGITFGKQF"
     gene            6523..6951
                     /locus_tag="U6309_001752"
     CDS             6523..6951
                     /locus_tag="U6309_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505192.1"
                     /translation="MYLSRCISHGAIAGIIAGIVFTFFLVMGGMLETLGSMINMPTKE
                     GGLLVHAAMSIGSGIAFALVLDWLMHSWLSAILLGLLFGFAMWVGGPMTVLPYFASGV
                     PLFAKWTMEGIRQNIPPLVGHLVYGLILGVTYYAFKRRNK"
     gene            7182..8027
                     /locus_tag="U6309_001753"
     CDS             7182..8027
                     /locus_tag="U6309_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN5505193.1"
                     /translation="MKYQQKLENMIAFIGKHLDEELSLGSLSEIFCISKFHFHRLFTA
                     FTGLSLQQYIKWLRLKRAAHQLVVEKDQSVINIAINAGFESHEAFSRAFKKACGFSPS
                     QFRQGFGRSYWEQPPYCLPRQGRIDMKVDIKSIDKIRLAVIEHKGDPKLLGESINKLV
                     SWAKSQSINLKPRPGEAFALAYDDPKTTPPSEFRIDLGIKVPENLKLDGVIEKFLPSG
                     RYAVTVHKGSRNNIGDVVYYLYRDWLPNTSEQLGDLPCIFCYYNFDHEVAETELLTEC
                     WLLLK"
     gene            complement(8265..9113)
                     /locus_tag="U6309_001754"
     CDS             complement(8265..9113)
                     /locus_tag="U6309_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505194.1"
                     /translation="MCKEINIIWVGPQRLPYKYLQHLKKWHNLNPDHQINFITDEHSS
                     SIQLPGINTIHWKLCFDLTDVRQEFLLKEAERALQEKAYATLSNIVRKVKIFNDGGYY
                     FDTDIVPMEKLPTVTENTPFLSQPKASYGEEFAKHRFVVSALYGSKHSPVFNEALTVM
                     HETYQVKSLQDAIKENPEEADYLRSYYSASLMLSRSVKRLYTNEQDYQKMIEDCQNEF
                     GNPTAFQIIQDGSWGEGLNAVLMESKHLAATAIQRFFRAAQALDQVNANQSTTNTKPS
                     EKPRLF"
     gene            complement(9291..11549)
                     /locus_tag="U6309_001755"
     CDS             complement(9291..11549)
                     /locus_tag="U6309_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HEN5505195.1"
                     /translation="MFAEIPKIQEQLHDVLEYSQSMASLWMQSIDKSLEQTNKVTQSL
                     INLNQQQVYPYPNILNDAIEYTVDFMQRSILFWDIMRKRGNQYLKHEQEGQPPVLIFS
                     YNMLMDGRHFERPVNYALVEITPPEGIQIDPAKRPYVIVDPRAGHGAGISGFKDASQV
                     GLALRAGHPVYVIIFFPVPEPNQTLVDVTAAHEKFLTEVALRHPQSPKPCLIGNCQGG
                     WAILTLMAANPDVAGVAVVNGAPLSYWGGENGKNPMRYIGGIWGGSWIAQMAGDLGNG
                     KFDGANLVMNFEMANPKVTYWSKYYNLFANLDKEEIRFLNFERWWGGFSLLNVNEMRG
                     IIDNLFIGNKLVHGKIPLGQSGNNLDLRNISVPVIIFCSEGDNITPPQQALNWVSDLY
                     SNTLEIKLDGQVIVYLIHKSVGHLGIFVSSEVAKKEHNQIIDLLNYIEHLAPGLYELK
                     LNETKENPKSPPHYLAYIEERSINDIRKGQENNVAIFNLVRMVSEYNAMSYDLFMAPI
                     IRNISNEYTAELIRKLHPLRQNQYLLSDLNPLVHPVSWISPFIRQNRIKISENNLFLT
                     QQLNFSKLINALWDFFSASRDNTVELMFYAVYGYIQLLAPFDPRRDMIVHSPEKDNQE
                     KITQSIIAHISDGGVPEAILRILLLLVKIQGYIIGANLPDVIQKLRECNALKHLDKNG
                     FKQIVHSQTIMIEHDPELAFNTIPHLLKSQEERSMVIQFIEDILKSLKVSPSEEYKKK
                     FQRIKELLQNQA"
     gene            complement(11614..11781)
                     /locus_tag="U6309_001756"
     CDS             complement(11614..11781)
                     /locus_tag="U6309_001756"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505196.1"
                     /translation="MHNASYSPWPTSRNVLKGLKTNANGFIIVLEEDTSLKRKPPYEN
                     MKKEENNGNVH"
     gene            12082..>13242
                     /locus_tag="U6309_001757"
     CDS             12082..>13242
                     /locus_tag="U6309_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2160 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505197.1"
                     /translation="MFSANQSLDFAWLSMQMFLINLLKDKIMRYAVTYCAMDHEFSGN
                     PFWHSCILLSQWDNNGKIEVVDNWGFYGVPSTVRDTWLSKLKIKLGLDVDLKGNHGML
                     RHEELRYLDVGYGLHGVTFELTKEKFDLLQLKCQTMLEEQKQAVKEVVESQGIAGKQK
                     YRIYEHEEYSPLIFALEKIKAKQKGHQPRLKPFELNLSFTLWGPALNQSYTCKSLMVD
                     LLKGILTQEQIARITEAGKHPTVPRYSGKLERIYLHSSGPLREHKKSSGAIVHYRDLQ
                     DEGVKLHWTLPPQEIEALSGDTIKLLEISEEYCDEVKQAISKLQKLEWLFIDAKLPEK
                     YKPYQDDLVARIRQCYEAFAQVEPKKAKSTATGWMGFAFSLLSLPRDLDEKCL"
BASE COUNT         3964 a         2255 c         2610 g         4413 t
ORIGIN      
        1 acggtttata ggcactttaa ttaacctctt taattgaaaa ttactttttt aggatttctg
       61 aatatcaata agatatgggg gtatttagaa gttttgtttt tcaaaagact ccttgttttt
      121 gattttcttc atcaaaaaat ttcacaaaga aggttttgga aagcttggga ttggcttgat
      181 tgatgcgtgt aaattcagcc gtatatccac attttttgta aaattccgga gcctggaaag
      241 cgctggtaca taaattgata ttattaaatc cttgcccttt aaaatgatct tcaagagttt
      301 gcagtagttt tctgccatat cctttaccac gatatgtttt atctacccag atatcatcta
      361 tgtatatttc cgcaaaggca gtaaaggcat taataacgcc acaaggtatc ccttctgcag
      421 atagtactat tgaaaaacgc ttgtagttca catcaatgcc atgagttcgt tcataggcta
      481 tcagatcttc tgtcatcttt ttttccagag attcagggat actatccacg aaatctattg
      541 taataggttt ttggggaatg gaatgacaag atttgttctg ctgttttgta ttaaaattta
      601 aaataatgcg ttgggtttgg tgaatatcat aattcacagt ctttatctgc tctttatact
      661 gattaagggt ataggtcatt accgcttgac gccaaaacga taaagccggt atgttttctg
      721 gaataaccga tatttcccat tcacctggat gagtacgcca taattgatgg gctactgatt
      781 ggccaatacc tttcccttgg aatttagcca ggatgaaaaa ttcccccata ttccaatcaa
      841 cagcttgggt tccaaattta tttaataaag caaaaccagc cagttcattt tcaatttgaa
      901 taaggaaggc ttttcgttcg gggtcagtaa aataaatttt gaaatcataa ctttcataaa
      961 gtccatctgg tggacatgcc cattcttccg agataaaacc gcaataacgt gacatatcat
     1021 aaacataaaa tcgtgccata ttttgaataa ctggatagtc actaagtgtt gcttcaatta
     1081 acttgggctg taataaatta tcagtgctca tgaatattta tactggagtg agtttcttaa
     1141 ctatacggat aaggtatcaa agtgtgctgt ggttttatag agcgattgtt gttatttgca
     1201 taattaatca gccattttgg tagattgtct gtaataaata aaagatttcc acgaatgaaa
     1261 gtggttaatt cttaatattg attttgataa ttataggttt ctcaatgata tttttagtga
     1321 gaatgtctcc attgcccttc ggcaatacct ctatagatca cataagtaga gagaattaaa
     1381 aggatatgat agatatcatt atcatagcga tattctaaag gtatttcatg accagtgaga
     1441 tagttttcaa tatgtcttgt aactatccat atcaggtttg atataacgac catgacaaag
     1501 ccaatcctga cttttgcttg cgaataataa gccgcaatca aacatacaag gacgacagga
     1561 agggcaatgt aggtataatt tccatcgtgg gtcaggaaca gataagtaaa tatgatggat
     1621 agaaatacta cgataactcg gcacaaatcg ggcatttgta ttgattggac aagcagataa
     1681 ttcagcatga ttacaagcaa actaatcgca atccaggctg gtggtaaatg caaatgagaa
     1741 gggtagtaat ggatataaac tcccaatacc ttgagaacaa ggaggtttaa gaaaaaaaat
     1801 acaatcaaaa gtgaaaaagt atgatctttt ttaaagtagt gaatacaggc accaattaag
     1861 tacaataata ggaaaaacgc atgaactgca ttagagatag catttgttgt tgttgcgtcc
     1921 cataccattc tgtatctccc tttgaaaata cagctatcag ggtctgatat cctgaagttt
     1981 gttaatgtac taatagtaat tgtagcttgt agaagattgg gatgtgtgat atcagtcact
     2041 taaaagccat actacaagct atgattagtc attactccaa tcgtttatgt tctctttaaa
     2101 caaaaaatac gaagacgatg ttcatcggga tcaacaccaa caaatgtgta cccaaaatcc
     2161 atttgtgtcg gaggttgaat aatagaaatt tctttatcag accattgctt atatatctca
     2221 tcgaccattt cattgctact gacctgaaag gacagctcca taccccctcc tgtttgatga
     2281 gctgtaggct taatctcttc tcgcgcccat aatccaagac gaagccctgt tttcatgaca
     2341 aacatagcga atgtcggtga agattcaata ggttgagtgt ctaacagatt tttataaaat
     2401 tcttcacttt ttgccgggtt tttgacgtaa aataaaacca aattagggtc acttaacatg
     2461 ttattttcct tattttttat taatcatcgc cagtataaac aaggctaatg acagattttg
     2521 tcagtagtgt tttaagtgcc taatattgac cttcttgata ataaaaaata cacgaaacag
     2581 gattttggtg ttattatgaa aacaaaattt agcttatcct ggctcaaaaa ttttgatgag
     2641 tttattttat gaacagccgt attgaaactt tccatcaaat ggagcggcat tttttttcac
     2701 tgattagtat ggagctttta ttattggaag gattgaaagc ttgtaacagt ggaatgccaa
     2761 ttcctgctct aaatcccgtg ttttattcag gtaaatcgac tctgcttggg gcagagattt
     2821 atcaatgcaa gcgcttttat gagtctcatt caaccccttg ggcacttgtg gttcctgact
     2881 atttatcgga tacagaagca gataaggtgc tcgctcaatc aggattctta tctattgatc
     2941 aaggcgttgc catgtatttg tcaattagtg atcttggaat ttctgagctt gtatctgact
     3001 tgttgataag gcggatggat aatgagatgg atacctggat tttacccact atccctgcat
     3061 ttgaatcaac ggaagagatt gcaaagattt acagggtgag acatcaagag gctattgaga
     3121 aatcccctgg gatttatcat ttttccgggt ttattcaaga gcaaattgtc tgctcattaa
     3181 ctctgactgt tatgggggat agtgcgcgaa ttgatgacgt agccactttt cccaaacatc
     3241 agaaaaaagg gtatgcaaca caattaattt tatctgtact tcagactctc aaagacacca
     3301 atatttcctg gtgcttttta gaggcatctg ctgatggttt gaatatttat aaaaagattg
     3361 gttttcgtga actgtttaaa aatctttatt atgaagagca atgccaatat gagcaaaact
     3421 gagagacttt ttcatctttt gcaaattttg cggcgttacc gttatcctct tagcggacaa
     3481 tttcttgcca atgagctggg tgtaagtacc cgtaccatct atcgggacat tgcggtttta
     3541 caggctcaag gtgctgatat agagggtgaa ccaggattgg gttatgtgtt aaagccaggt
     3601 tttatgttac cgcctttgat gtttagtgaa gaggaaattg aggctcttgt tttaggctcc
     3661 agatgggtgg ctaaaaaaac ggatactcac ttgcaacagg ccgcacataa tgccttggct
     3721 aaaatcgctt ctgtacttcc tgccgatttg cggcagcaat tggagctatc cagtttatta
     3781 atagggcctg gtgaagtgat ttcaggagaa gatgaatccc tggccttagt caggaaggca
     3841 atacgaaaag aactgaaatt gtttattcaa taccatgact taaaaggaaa tgtttcaacg
     3901 cgtacgattt ggccaattgg acttgggtat tttgatcaag taagagtttt gattgcctgg
     3961 tgtgaattaa aaaaggattt tcgtcatttt agagccgata gaattgtaga tgttgaaaac
     4021 ttgggggtct cttatgcaaa gcgacgctca gtgttgcttc atgattggcg aaagcaaaat
     4081 aatatcccag gttgatgaca acacgatgtt attggcgcgt taacttgaac aggtaaaatt
     4141 taatgccggg aatcaaatat tactttcttc ccggattaag attgcttctt atgcgaaaga
     4201 cgagctgaac tcccattatt cctgagtgag agaacctcgt atttcaaacg gttttacatc
     4261 aaattttgaa tggcaatggc tgcgacacca acagcaattg ctcccaatgt ttctcgattt
     4321 ggcaagtgct ctttaaagaa accataataa tgatttaaat ttgaagagaa atcactgcca
     4381 ttggtttcgt gtttgatttc tgatagcact tttgtaacgg aaggctcttt ttgttcaaca
     4441 gcagtgtcct tttgtagttt ttcttgcagt ttttgttcta acctttgttc aagtttgtgt
     4501 tctaactgtt tcaattgata ttcgttgatt tgcttttgat tcttatcgca ttcaatgtga
     4561 gtttcttcca gttgtttttt taattcagct cgataggcat ccgcggtgtc gaaggggtga
     4621 aattcttgct tcagctcatg ttcagcatta atgacttgct catagctggt ttttaaattt
     4681 ttgataaatg aatcattaga aaaccaggac atgatccagt cgaaaaattt tgtaaaaaac
     4741 catttgttat tccattcatt gtttaattta tggtggtttt gcgaaaggag atgatagtct
     4801 gccagtttct ttgcaagagc atctattttg acaatcaatt gatttctttt atttcttgaa
     4861 tcttcaaggg atttttcaag cttggctagc tcatcggaca ggttggattc ttttgattct
     4921 ttaagctgtt ttaactcgat tggttcactt tttgcagttt tatcggcatg aaaattctcg
     4981 aacaatcttg ttgtatactc ttttgctgac tcgtcatttt tctttatgaa ttcagattca
     5041 ttaaaggcaa tattttgact ttgacaatat cgtctaattt ctcccaacat cgcttcggct
     5101 tgagcacggg tcgaaatttt cttttcatta acaccatgga ttacctttga tctaaaccaa
     5161 ttgatagaac cttggtattc gacattttca tagttcttaa ggtgctgata gtaaacgatt
     5221 gcaaaaatga gggtttcatt gttagttatt ttgggtaaat aaaattcata ttttttttca
     5281 ggagtaacaa atgggactcg atatccgaat aatttggctt tttcgtgcag tcgatctcca
     5341 acctgttgga tttgagtaat ccccagttgt tctattgtca agccaatctc gatcataagg
     5401 tccgcgatgg ctatttgatt ggagtattct ttgttgtcta cagatgaacg aagttgatca
     5461 atgcaatctt taaattgttg ccaataagtt gttattgaaa tcatatcaat cattatttaa
     5521 gaaaaaagag tcataaattt acaccggtct tctaaaaaaa gctattattt agtcgtgatt
     5581 atcaatcaga aaatttttaa aaagagagaa agcgggacaa ttcatataga ggaaaatagc
     5641 tatttaaaat tgtttgccaa aagtaatacc aaagatcagc tctttttcac ccccttcaat
     5701 aaaatcaagg tctatattcg gagtgatttc tatatccgga gggaaatgta tgatatatcc
     5761 aatccccaat cgaccaacca tgcggttggg ttcatctttt tcatatctaa ttccagggcc
     5821 tatgccaaaa gtcaggttcc tgaatacgtt taatgtacca agacctatca gttctacttg
     5881 attattatgt tccacattaa cgggtgtatg ttcaataacc gcactaaaac caaaaggcat
     5941 ggagaggact cggtggtact caaatccata ggtaaacgct tcatcgtttt gagcatgatt
     6001 tccacctaca aaaacaccaa ggagattttt gtttttttcc ctcacgtgcg gtttgtttga
     6061 gaaatctgga gccgaattta ccgggtgtat tgaacaaaaa aggcatgcaa tccccagagt
     6121 gatagttatt attttcttac catttattac cgacatgagt tctcaataca gggttatcta
     6181 tttctttatt aaatcgcaaa atggctaata tttaaagaac atttgaatcg atatgttata
     6241 taaaagtctt tattaacaat aagatgttga tgatgtagtt ggcaaaaaat tttttaagac
     6301 attgccatta atttttctac cttaattgtt gattattaag taaactgatg attttgacag
     6361 gactttcgaa aaactccaag gtcaatgcaa aaaatgtttt taatgaggga gtttagataa
     6421 actaaatgac cgaattaaaa acttttttta acatagagat tgggattttt cgtaagtcct
     6481 gtttgaatgg taaactggga tctatttaat aaggagattg gcatgtatct ttcaagatgt
     6541 atttcgcatg gcgctattgc aggaattata gcagggattg tttttacctt ttttttagtt
     6601 atggggggca tgcttgaaac attgggaagt atgattaata tgccaacaaa agaagggggt
     6661 cttttggtgc atgctgcaat gagcattggc agtgggatcg cttttgccct ggttctggat
     6721 tggttgatgc actcctggct ctcagccatt ctccttggat tattgttcgg ttttgcaatg
     6781 tgggtcggcg gcccaatgac cgtgttaccc tatttcgctt caggggtgcc tttgtttgca
     6841 aaatggacta tggaaggaat tagacaaaac ataccccctt tggttgggca tcttgtgtat
     6901 gggttgatat tgggtgttac ttattatgct ttcaagcgca ggaataagtg actgcatttt
     6961 tcatgcgtaa caaattgatt ttattaaaga tataatgaca tcaaaaggtg gtgattgttt
     7021 tttatggcaa tcgccacctc aatttagcaa aaagaattgc atacatcaca atcaagcgta
     7081 gtttattttt ctcaattaaa tcagcaaaaa tggtcaaata ttttcagtaa gaaagcaata
     7141 ttcttattct gtaaatcaag ttaataatcg gaaacaagaa agtgaaatat caacaaaaat
     7201 tggagaatat gattgctttc attggaaagc atctcgatga agagctttct cttggaagct
     7261 taagtgaaat tttttgtata tcaaaatttc actttcatcg cttatttact gcatttaccg
     7321 ggttgtcttt gcagcagtat attaaatggc tacgcttaaa acgcgctgct catcagcttg
     7381 ttgtcgagaa agaccagtcc gtcattaaca ttgcaatcaa tgcgggtttt gaatcccatg
     7441 aagcgttttc cagggctttt aagaaagctt gtggatttag cccaagtcag tttaggcaag
     7501 gttttgggag gtcatattgg gagcagccac cgtattgttt gcctagacaa ggtagaattg
     7561 atatgaaagt agatattaaa agtattgata agatacgatt agctgtaatt gagcacaaag
     7621 gtgatcctaa attgttaggt gaaagcatta acaagttagt gtcctgggca aaatctcaat
     7681 cgattaatct taaaccacga cccggagaag cgtttgctct ggcttatgat gaccctaaaa
     7741 caacaccacc atctgaattt cgtatcgatc ttggcatcaa ggtccccgaa aatttgaaac
     7801 tggatggtgt gattgaaaag tttctgccat caggccgtta tgcagtaaca gtacacaaag
     7861 gttctcgtaa taatattggt gatgtggttt attatcttta tcgagattgg ttacctaata
     7921 cctcagaaca attaggagat ttaccctgta tcttctgtta ttacaatttt gatcatgaag
     7981 tagctgaaac ggaactttta acggaatgtt ggcttttgtt aaaataatcc gctttatacg
     8041 ccagtcggaa tagctgataa caataggctg ggtataacct gcgtcttttt aagttgtagt
     8101 aattttattt ctaaataact tgtcaaacaa cctatgtgcc tcagcttgtc catgaggcat
     8161 tcataacaca ttttgtagat gctactgaca agcagtggta tgcagaaaat ccgtccttta
     8221 aaacttaagt aactatacat taaatccagt tactcaattt atcttcaaaa gagtcttggt
     8281 ttttcagagg gtttagtgtt ggttgttgac tgattggcat tcacttgatc cagagcttgc
     8341 gccgcccgaa aaaatctctg gatggcagtt gctgccaggt gtttactttc catcaaaaca
     8401 gcatttaatc cctctcccca tgaaccatct tgtataattt gaaacgcagt agggtttcca
     8461 aactcatttt ggcaatcttc tatcattttt tgataatcct gttcatttgt ataaagtcgt
     8521 ttaacagaac gggataacat aagggaggct gaataatagg agcgaaggta gtctgcttcc
     8581 tccgggtttt ctttaatggc atcttgcaat gatttcacct ggtaagtttc atgcatgact
     8641 gtcaatgctt cattgaatac aggagagtgc ttgcttccat aaagtgcgga aacaacaaaa
     8701 cggtgtttgg caaattcttc gccataactt gctttaggtt gtgataagaa aggagtgttt
     8761 tcagtgactg taggtaattt ttccatagga acgatatcag tatcaaaata atacccgcca
     8821 tcattaaaaa ttttaacttt gcgaacgata ttcgataaag tggcataggc tttttcctgg
     8881 agagcacgct ccgcttcttt cagcaaaaac tcttgtcgaa catccgtcag gtcaaaacat
     8941 agtttccagt gaatggtatt tattccggga agctgaatag aagaagaatg ttcatcagtg
     9001 atgaaattga tttgatggtc tgggtttaaa ttatgccatt tttttaaatg ctgtagatat
     9061 ttgtagggaa ggcgttgtgg tcctacccaa atgatattaa tttctttaca catattgtgt
     9121 ttaccaccaa ttagaaaaaa attgctttta tagcaacgta ttcataatgt caataaaata
     9181 aacaattgag atggggggat tttatttgtc tgaaagatga aggattaatt ttccaatagg
     9241 atatctatag gtttaggctt gattgagagc agtatggccc aattcggaaa tcaagcctgg
     9301 ttttgtaaca gttcttttat tctttgaaat ttctttttat attcttcgga aggggatact
     9361 tttaaagatt ttaatatatc ttcaatgaat tgaatgacca ttgagcgctc ttcttgtgat
     9421 ttcagtaaat gaggtattgt attaaatgca agttcaggat catgctctat cattatggtt
     9481 tgtgagtgca caatttgttt aaatccattt ttatctaaat gtttaagagc attgcattca
     9541 cgtagttttt gaatgacatc aggtaagtta gcgccaatga tatagccttg aattttcact
     9601 aataatagta atatacgcaa aatggcctct ggtacacctc catcactaat atgggcaata
     9661 attgattgag ttattttttc ctgattatct ttttcagggg aatgaacaat catatctctt
     9721 ctgggatcaa atggagctaa taattgaata tagccataga cagcataaaa cattagttcg
     9781 acagtattat cacgagatgc gctgaagaaa tcccataatg cgtttataag ctttgaaaaa
     9841 tttaattgct gagttaaaaa gagattattt tcagaaattt taatgcgatt ttgacgaata
     9901 aaaggtgata tccatgatac agggtgcact aatggattca gatcactcaa taaatactga
     9961 ttctgcctta gtgggtgtaa ttttcgaatc aattctgcag tgtattcgtt actaatatta
    10021 cggattatcg gagccataaa caagtcatag ctcatggcat tgtattcaga taccatacga
    10081 acaaggttga agattgctac attattctcc tgtcctttcc tgatgtcgtt aatagagcgc
    10141 tcttcaatat aagcgaggta atgtgggggt gacttgggat tttcttttgt ttcattcagt
    10201 tttagttcat ataaacccgg agccaaatgt tctatgtaat ttaataaatc aataatctgg
    10261 ttatgttctt ttttagctac ctcacttgag acaaaaatac ctaaatgacc aacactctta
    10321 tggataagat aaacaataac ttgcccatcc aatttgattt ccagagtatt tgaataaaga
    10381 tcagaaaccc aatttaaggc ttgttgaggt ggtgtaatgt tgtcgccttc cgagcaaaaa
    10441 ataataacag gcacgctaat gtttcttaaa tccaggttat tacccgattg ccccaagggg
    10501 attttgccgt ggaccagttt gttaccaata aacaaattat caattattcc tctcatttcg
    10561 ttcacattca aaagagaaaa acctccccac catcgctcaa aatttaaaaa tcggatttct
    10621 tctttatcaa gattggcaaa gagattgtaa tatttgctcc agtaagtcac tttaggatta
    10681 gccatttcga agttcattac caaattagcc ccgtcaaatt tgccgttacc caaatcacca
    10741 gccatctgtg cgatccagct ccctccccaa attcctccga tgtagcgcat gggatttttt
    10801 ccattttccc caccccaata ggaaagagga gcgccattga ctacagcgac ccctgctaca
    10861 tctgggttag cggccataag ggttaaaata gcccatcctc cctgacaatt accaattaag
    10921 cagggtttgg gactttgagg gtgacgcaag gctacttcag ttaaaaattt ttcgtgggct
    10981 gcagtgacat caaccagtgt ttgattgggt tcaggaacag ggaaaaagat aataacgtaa
    11041 acggggtgtc ctgcccttaa ggcaagtcct acttgtgacg cgtctttaaa accgctgatt
    11101 cctgcgccat gacccgcccg cggatcaaca attacataag gtctttttgc tggatcaatc
    11161 tggatgcctt cagggggggt gatttccacc agtgcataat ttacaggtct ctcaaaatgt
    11221 ctgccatcca tgagcatatt ataagaaaaa atcagaacag gaggttgtcc ttcttgttca
    11281 tgcttgagat attgattgcc tcgtttacgc atgatgtccc aaaacagaat gcttctctgc
    11341 atgaaatcta cagtatattc aatggcatca tttaatatgt tgggataagg atatacctgt
    11401 tgctgattta agttaattaa ggattgcgtc accttgtttg tttgttcaag gcttttatca
    11461 attgattgca tccaaagaga agccatggat tgggaatatt caagaacgtc atggagttgt
    11521 tcttgaattt tgggtatttc ggcaaacatc tttttctcct gtgcttctca actcggggtt
    11581 ctttaactcc atatcattaa caaattttga ctatcagtgt acgttaccat tattctcttc
    11641 ttttttcata ttttcgtaag gcggttttct ctttagagaa gtatcctctt caagaacgat
    11701 aataaaacca ttcgcatttg tttttagccc ttttaacacg ttccggcttg ttggccaggg
    11761 cgaataggag gcattatgca tcgagcattt aaatccattt gttaatcact tttaaatcat
    11821 tatagacagt tattgtcaaa acagtaaatg cagcatgttc actgaaattc gattgttgcg
    11881 ctttagattt ttatcaattt tgtttttagt ttgaaaggtt aaaaatttat ttctactaat
    11941 tgatatgtta ggcagttggg tttgctttta ttgggaaatt tccagggatt atctcattat
    12001 ctgtttagcc gttgacggca atatggggat ctgtaatagt ttcataggat tgtattatgt
    12061 gagtcaggtt taagcaattc actgttctca gctaatcaat ctctagattt tgcctggtta
    12121 tccatgcaga tgtttttaat caatcttctt aaggataaaa taatgagata tgcagttacc
    12181 tattgtgcaa tggatcatga gtttagtggt aatccttttt ggcatagttg cattttatta
    12241 tctcaatggg ataacaatgg aaagattgag gtagtcgata attggggttt ttatggtgtg
    12301 ccatctactg ttcgtgatac gtggttaagt aaactgaaaa tcaagttggg tctggatgtt
    12361 gatttgaagg gcaatcatgg tatgttacga cacgaagaac tgcgttattt ggatgttggc
    12421 tatggtttac atggagttac ttttgagtta accaaggaaa aatttgattt attgcagttg
    12481 aaatgccaaa caatgttgga agaacagaag caagcagtta aggaagttgt tgaatcacag
    12541 gggattgctg gtaaacaaaa ataccgtatt tatgagcatg aagagtattc cccgttaatt
    12601 tttgctttgg aaaaaatcaa ggcaaaacaa aaaggccatc agccaaggct taaaccattt
    12661 gagttaaatt tgtcatttac cctgtggggg ccggctttaa atcaatccta tacttgtaaa
    12721 tcactgatgg ttgatttatt aaaaggcata ctgactcaag agcaaattgc ccgaattact
    12781 gaagcaggaa aacaccctac cgtaccaaga tacagtggta aattggaaag aatttatttg
    12841 catagcagcg ggccattacg tgaacataaa aaatcttccg gtgcgattgt tcattatcgt
    12901 gatttgcagg acgagggagt caaattacat tggacattgc ctccacaaga aattgaagcg
    12961 ttatccggag ataccatcaa attactggaa ataagcgagg agtattgtga tgaggtaaaa
    13021 caagcgataa gtaaactgca aaaattggag tggttattta ttgatgccaa attaccagag
    13081 aaatataagc cttatcaaga cgatttggtt gctcgtattc ggcaatgtta tgaggctttc
    13141 gctcaggttg aacctaaaaa ggctaagtca acagccacag gctggatggg attcgccttt
    13201 tcattgctct ctttgccaag agatcttgat gaaaagtgtc tt
//
LOCUS       DASLOE010000080        13089 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.080, whole genome shotgun sequence.
ACCESSION   DASLOE010000080 DASLOE010000000
VERSION     DASLOE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13089
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.080"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(138..641)
                     /locus_tag="U6309_001758"
     CDS             complement(138..641)
                     /locus_tag="U6309_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505198.1"
                     /translation="MTKILICLGLLLSSNLLMAKPLSQLDSANLLPCFNIEQAERIGK
                     QINKLLQHEFCEENINPKKFASISHNILPKIMTETFLGVTPPENWQQLSDDIIKNCIA
                     NKNLCKKAARKELEECIKPRIPLILIQFGPWLSQNCPQLNKSLIEQWPNKQTTLKKII
                     NENKSVE"
     gene            complement(751..1797)
                     /locus_tag="U6309_001759"
     CDS             complement(751..1797)
                     /locus_tag="U6309_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN5505199.1"
                     /translation="MNQKSFQWSVVGAGPAGIAAVGKLLDYGIAPSEILWIDPHFKVG
                     DLGQLWRNVSSNTTVKLFSSFLHAANSFSYKKAPSTFRLNNLDPDNTCLLSDVAEPLQ
                     WVTSILVNQVIAEETVIHSLTLSGGCWTLCSDTQQYRAKNVILATGAVPSSLNYPGVN
                     VVPFEIAIDKQRLAHFVDTNETYGVFGSSHSAIMILRSLIELGVKKVINFYRSPCRYA
                     INMGNWILFDNTGLKGDTAAWARKHIDGVLPANLVRYYPSELNIARHLPECDKVIYAV
                     GFERRKNMTIDNYEHVTPNPHVGIIGPGLFGLGIAYPELSVDPFGTIEHQVGLWKFMV
                     YLCKVLPVWFKYPA"
     gene            complement(2101..3063)
                     /locus_tag="U6309_001760"
     CDS             complement(2101..3063)
                     /locus_tag="U6309_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEN5505200.1"
                     /translation="MKKWGLAIVALMLFNQITYASQSWNQWVAGVREEALQQGIPPSL
                     FDEAFAGIHEPSRKIKGFLRSQPEHRLTFMKYRNTRADNYRIVIGRKEYKKNKEILEE
                     IAQQYGVNPCFIVSFWGMETSYGSYMGNFPVINALATLAYDSNRKDFFRKELFLALHI
                     LKGGHVSLADFKGEWAGASGQPQFLPSSWVKYAVDYDGDGRKDIWKSKPDVFASIANY
                     MKQNGWQAGEPWAIQVKLPAKFDMKLEGKGIVKPVSEWNALGVRTESGEPLPYQNLQA
                     SIVQPYGGPTFLAFPNYKMILRYNNSIYYAGAIGYMADKICQGQ"
     gene            3593..4771
                     /locus_tag="U6309_001761"
     CDS             3593..4771
                     /locus_tag="U6309_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN5505201.1"
                     /translation="MHIKNIFTWNLMGAVILSMPLFAGEQVKLKSIDSLIDKYIEKYK
                     NEELVTGVGATFLSGTEIISTAHGFENNKRKKRLTNNAYFQIGSITKSFTAALILKLE
                     AEGKLNINQTIGDWLPEYDDWKGITIKQLLNMTSTIPGYATIPTLADSITDNPKKQWT
                     SKELVDLAYGYKHPTSGYNYSDTNYLILGMIIEKVTHDSFHNQLQKKILKPLQLKETY
                     YITDSYTPYFLKKMPESYYYGRTVENLKHGQKSTNDNLSMAGPAGAIVATTSDVAKWI
                     KALFSSDFLLPKQQMELKSLISMKTGTPISHVDSEEPNGFGLGVVQGYKPQLGKYWYY
                     EGDTVGFRVLYIYHPCNEKIVVIALNSSPFVDEAAGDHSGDLLLELYKNAFGISQKCE
                     "
     gene            5005..5424
                     /locus_tag="U6309_001762"
     CDS             5005..5424
                     /locus_tag="U6309_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HEN5505202.1"
                     /translation="MTNIDVRWQQRLNNYARALQQLSLAVNLAQTRPLSDLEKQGLIQ
                     AFEFTHELAWNVMKDYFFFQGNSAITGSRDATRESFNKGLIKEGEIWMEMIKSRNQTS
                     HTYNQSVADEIVKNIINFYHTSFQAFLEKMQGLKEHE"
     gene            5417..5743
                     /locus_tag="U6309_001763"
     CDS             5417..5743
                     /locus_tag="U6309_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN5505203.1"
                     /translation="MNEQLNHLYGLPSHAIEALKCVFKEYSQIDNAILYGSRAKGTYH
                     QGSDIDLCLTGNLLGITELLAIENKIDDLLLPWKVDISLKHTIDNPDLLEHIERAGIL
                     FYTKES"
     gene            complement(5816..7009)
                     /locus_tag="U6309_001764"
     CDS             complement(5816..7009)
                     /locus_tag="U6309_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN5505204.1"
                     /translation="MNFLNHCQQALVNLTASKLRSLLAVLGILVGTAAVVALISCGQL
                     ATEKALEQFKALGTDLLAVSVYQKVPSKSHSESDSLTIDQWQQIAERIPYVLRIAPYS
                     TAYQTLSFNGKVMQGAVIGADESLADIIHVTLAQGHFVSFLDSFEHFCVIGDSLAQQI
                     KEITLDDPIGKQLRIGQSLYTIIGVAERWKENGFFNEDINQAVIIPLAGISLVSKDAK
                     INNAVLLLKPDSPIDEVINEIKEIINSQAPKLSVFPRSAKQIIASMENQGRIFTLLLA
                     VIGGISLLVGGIGVMNVMLVSVSERKKEIGIRKAVGAKNREIQALFLVESVMLSLLGG
                     VLGVILGLIFTRIVAYFSDWTFTIYLLPPIAGFLVSAATGIFFGFYPARRASKLEPMV
                     SLRSE"
     gene            complement(7006..7695)
                     /locus_tag="U6309_001765"
     CDS             complement(7006..7695)
                     /locus_tag="U6309_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN5505205.1"
                     /translation="MIDRPLIQLSDLVKTYHLEGISTTVLKAVSLTVNEGDLLAIVGA
                     SGSGKSTLMNIIGLLDKPDAGTYTLNNRNVASLSDDEAADLRNQNIGFVFQQFNLLPR
                     FTAMQNVALPLTYRGVNPTLIKEKVEQALDKVGMRQYIRHRPTQLSGGQQQRVAIARA
                     LVTDPQVILADEPTGALDSRTGSEVMNLFLALHQEGRTIIMVTHDEHVAAQCKRRITL
                     ADGAIIAESGQ"
     gene            complement(7688..8833)
                     /locus_tag="U6309_001766"
     CDS             complement(7688..8833)
                     /locus_tag="U6309_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEN5505206.1"
                     /translation="MNNYLKIFSIASLILMLVSCGSSDKPQTSSLKTYEVKPQPLHKT
                     LHFTGTVQPIRESSLTSPMEAVVETMHFHYGQMVKKGEIVLTLNSNELQKQYNDTLTD
                     YLKAKDSYSIAKAKFVGTEELWNAGLISKNNYLSEKSGLDTARVTLMQATRKLSEMLE
                     KMDDKNTQNISNLSLADFDKVRKILTSNHNLIHLKAPSDGIMLYPPKSGEDKNNRVTV
                     GSTIKSGQVIALIGDLKGISVEIDVPEIDIDKIRPGMEATISGVAFGRHQLKGKLVAV
                     NAQASNTSTGGLPSFTAVVEVNSLTPEQQSWIKVGMSASIELNVESNNQLLIPIAAVK
                     REKGSSVVHVQLAKGKVQKRMITTGAAQADSVVVESGLKSGDVVVYD"
     gene            complement(8830..10458)
                     /locus_tag="U6309_001767"
     CDS             complement(8830..10458)
                     /locus_tag="U6309_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEN5505207.1"
                     /translation="MKFLNKSTALLPVIILSGLISFHASSAKTSSNADNSLYSWTENP
                     SAEAQRVEQAEERLKAAIANPEYMLKNWIELPTSPESKKKTRHQLTIREAILLALRYN
                     PNIQNAELDRIIQRYQLRLANNEFELQYALGASGVVQKSTFEGIGSSTNHSFLASPEL
                     DLKTKIGTKASVSIDNNVSTNNSYNPVLNLSITQPLLRGFGKSANEAKLLDAIDKEWL
                     NKLSLQQSVMDQITQVITAYRALILSGNNLENQRLQLEEARKSYRINEKKIAAGQLEP
                     TGNIQQSYQIESLSLMVEQGENDFKLASQELLQMIGLDPDLHISVPSDVTIKEIIVPD
                     LQESIAIALSHNTQYLAQKMALRADERSYTVAKNQQLWQLDLAGTVQGGAVNDVTGTS
                     SGLSSIYNGNNITESARVTLTIPLNDIGRRSQLINAKVQLEKDKINLIAAKRALITSV
                     TNTINNIQSLAKRYELAQKQVKLAEQSYALEKKKQQAGISSALDVNNTQNQLIQAQSG
                     LIGAKIAYLNQLSALQRILGTTLDYWQIKLRYGG"
     gene            complement(10502..11332)
                     /locus_tag="U6309_001768"
     CDS             complement(10502..11332)
                     /locus_tag="U6309_001768"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEN5505208.1"
                     /translation="MPDYAIGDVQGCYDPLQRLLELIDFNEKEDCLWFVGDLVNRGPD
                     SLAVLRFIYSLPVKPKITLGNHDLHLLGLLFGGQPWKGHDDTLEEVMLADDGEELGHW
                     LRKQSLLCRSSELNIVMCHAGIAPLWDLSKAVDLANELEAVLSGDSYHEFFAQMYGNK
                     PDIWSDDLVGLDRLRVITNYFTRMRYCDAHGRLDLGYKGTLSKAPNHLYPWFEVPCRK
                     EIEMDIVFGHWAALMGRSSHPRIHAIDTGCLWGGQLTALRLQDRQRFSVPGYGVSRFE
                     "
     gene            complement(11325..12662)
                     /locus_tag="U6309_001769"
     CDS             complement(11325..12662)
                     /locus_tag="U6309_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HEN5505209.1"
                     /translation="MFHRQRKSSLSAQSKSLKDLIRMVTAEQFLSEEKRQVLLQKMRA
                     LSGLEASRYESLCFVLVENLVNYCQNLPETANSYYSQPGGLVDHALNRTEAALSLFKE
                     FTVQEQPDLLSEEQKLWQYALYSAAILQGLGKLFDEFRINLFDSNGQLLKQWNPLLES
                     LIQTGSYYDYQFEKEADVEFRCRLNLLLARTLMPASGFAWIASNPEVLAVWLALLNED
                     YRSAGTLGAILIRADAIAIQRYFLAFMARSGARRSGRYGRAVTFTGGVPESITEKEQA
                     IGVEFIQWIIKSLDEGRIMVNKAPLFMVPGGMLMCPEMFQLFVREHPEYKNWQAIQNG
                     FLSLGLHGRDAGGNVTSRFEQAHTQQMYTGVVFAGYGVALPNFVQVHHLNTGKIESMS
                     ALELIHKSQYTSQFTQQSSAIAIAPLQRLAANGQWQNIEQDMSQLTLGAKRGA"
     gene            complement(12699..>13089)
                     /locus_tag="U6309_001770"
     CDS             complement(12699..>13089)
                     /locus_tag="U6309_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HEN5505210.1"
                     /translation="FMLQKEVVERIAAAHGTKAYGRLSVMLQYHCEVEYLFDVPPEAF
                     EPRPKVDSAIVRLTPHRVSPFESVNTEKLENIVAKAFAMRRKTLTNNLKGIISLSQLN
                     DLGIDGGKRPEQISVAEYVQLAKFISN"
BASE COUNT         3804 a         2925 c         2556 g         3804 t
ORIGIN      
        1 gtttgtgctg ctcttgggcg caaacccatc gttttaaaaa cctggttgta gatcatatcg
       61 gtgagttata tttgatgaaa ttaggtaaat aaatgtggtt ttatcaggca taactctcgg
      121 aattaagcct gtctcgatta ctcgacactt ttattttcat taattatctt tttgagagtg
      181 gtttgtttat ttggccattg ttcaatcaaa gacttattca attgtggaca attttgggaa
      241 agccaggggc caaattgtat caggattaaa ggaattctcg gtttgatgca ttcttccaac
      301 tccttgcgag ctgctttttt gcatagattc ttgttggcga tgcagttttt tataatatcg
      361 tcgcttaatt gctgccagtt ttctggcggg gttactccta aaaatgtttc tgtcataatt
      421 ttgggcagga tattgtgact gatagaggca aatttctttg gattgatgtt ttcctcgcaa
      481 aattcatgct gtaagagctt attgatttgt tttccaatgc gttctgcttg ctctatgtta
      541 aaacagggga gcaaattagc agaatctaat tgcgacaatg gtttcgccat gagtaaattg
      601 ctgcttaaaa gcagtcctaa acaaattaat atcttcgtca tttttttatc cttggcgttt
      661 cctaaccatg tcaatataac agaatcttgc gagtctgtag aaaacccgcc gatgaagagt
      721 gggcgtgtga tgagttatca tctcgcaact ctaggcagga tatttgaacc agaccggcag
      781 tactttacac aaatacacca tgaacttcca taatcccacc tgatgctcaa tagtcccaaa
      841 cgggtcgaca ctgagttcag gataagctat gcccaagccg aataagcctg gccctataat
      901 gcccacatga ggattaggag taacgtgttc ataattatca atggtcatat ttttacgtct
      961 ttcaaaacct acggcgtaaa tcactttatc gcattctgga agatggcgcg caatatttaa
     1021 ttcgcttggg tagtagcgga caagattggc aggcaagaca ccgtcgatgt gtttcctggc
     1081 ccaagcagcc gtatcacctt tcaggcctgt gttatcgaat aaaatccaat tgcccatatt
     1141 gatggcataa cggcaagggg agcgataaaa gttaatcact ttctttaccc ctaattcgat
     1201 aagagagcgt aaaatcataa tggccgaatg agaagagcca aaaactccgt aagtctcatt
     1261 cgtatcaaca aaatgggcaa gacgctgttt atcgattgca atttcaaacg gtaccacgtt
     1321 aacacccggg tagttgaggc tggatgggac agcccctgtc gctaaaataa catttttcgc
     1381 tcggtattgt tgtgtgtcag agcataaagt ccagcaaccg cctgagagag tcaagctgtg
     1441 gatgacagtc tcttctgcaa taacctgatt caccagaatg ctggtaaccc attgcaaggg
     1501 ctccgcaaca tcactcaaca ggcaggtatt atcaggatcc aggttgttta aacgaaatgt
     1561 ggaaggggct tttttatagg aaaatgagtt tgcagcatgc agaaaacttg agaagagctt
     1621 gaccgtagta ttactggaaa cgttccgcca gagctgtcct aaatcaccca ctttaaaatg
     1681 aggatcaatc cataatattt cagaaggtgc aatcccataa tccaatagtt tacctactgc
     1741 agcaattccg gcaggtcccg cgccaacaac tgaccactga aaggatttct gattcatttt
     1801 aatacctaac gtgagttatg gctaaagagg atataacagc ccgtcgatac ccagggtacg
     1861 ttcgggctga ggagatgcct aagcatcgtc tcgaagcctt ggcatgaaac ataaaatccg
     1921 atgctaggct tcgagacggc ataaatgcct cctcagcccg agcggctcga gataacaact
     1981 gattttatcc ataactcacg ttacctatga tttataaagc ttaaaaagct tggcctatga
     2041 gaactccact gaacgcctgt ccagtggaat tcatcagtag atatttatgt ctccaacaaa
     2101 tcattgaccc tggcaaattt tatcagccat atacccgata gccccggcat aataaatgga
     2161 gttattataa cgtaggatca tcttgtagtt gggaaacgcc aggaaggtcg ggccaccata
     2221 aggctgtacg atactggcct gcaaattttg atagggcagg ggttcaccgc tttccgtgcg
     2281 cacacctaaa gcattccatt cgctgactgg tttgactatg cctttccctt ccaatttcat
     2341 atcaaacttg gcgggaagtt tcacttgaat agcccagggc tctccggcct gccagccgtt
     2401 ttgtttcatg taattcgcta tggaggcaaa aacgtcgggt ttgctcttcc atatatcttt
     2461 tcgtccatcc ccatcgtagt cgaccgcgta tttgacccag ctggatggta aaaattgagg
     2521 ttgaccagag gcgccagccc actccccttt gaaatcagcc aggctgacat gcccaccttt
     2581 caggatgtgt aaagcgagaa acaattcctt gcggaaaaag tctttacggt ttgaatcata
     2641 ggccaaagta gccagcgcat tgattaccgg gaaatttccc atgtaggaac cgtaactggt
     2701 ttccataccc caaaacgaca caataaagca aggattaact ccgtattgtt gggcaatctc
     2761 ttccagtatt tccttgtttt tcttgtactc ttttcttcca atcacaattc ggtaattgtc
     2821 tgctctggta ttacgatatt tcatgaacgt taacctgtgt tcaggttgag agcgtaaaaa
     2881 accttttatt ttccggcttg gttcatgaat gccggcaaag gcttcatcaa ataaactggg
     2941 tggaatccct tgttgtaagg cttcctcacg caccccggca acccattgat tccagctttg
     3001 acttgcatag gttatttggt taaataacat cagtgcaaca atagcgagcc cccacttttt
     3061 catagctctt cccctcagtt atatttattg tttaataata tgtatttcgc ccagcgtttc
     3121 tttctcttta attgttacaa aaaacaatcc gctcaaaaca tactggtatt cttaaaagat
     3181 agctcattta ttaaaaattg gcctgtctat tcgtcattct ctggcaatat accgacaaag
     3241 cttgaaccag aaagtcagac ctaacaataa gtattgtaca gaaagtaatc tttcatggaa
     3301 atctataatt agaacccgct cattagaaga aagtggggct ataaactagt cttggcctgc
     3361 gaagattgtt atgggatttt ttcgcatctt tttccattct attttttgtc aacatgataa
     3421 ctccattgat aacaaaatag attttaggag gccgtatgga gcacagcttc tttaacatgc
     3481 agagattaaa taccataacc caggtattga tatgcttgta tgtaagtaaa ctggaataaa
     3541 aagaaattag tgttcatagt ataactgagg ctttgatcca caggagttaa atatgcatat
     3601 caaaaatata ttcacatgga atttaatggg cgcagtaatt ctatctatgc ccttatttgc
     3661 gggcgagcaa gttaaattga aatcaattga ctcattaatt gataaataca ttgagaaata
     3721 taaaaatgaa gagctggtta caggtgtggg tgcgactttt ttaagcggaa ctgaaataat
     3781 atcaacagcc catggttttg agaacaataa aagaaaaaaa cgccttacaa ataatgcata
     3841 ttttcaaatt ggcagtataa ccaaatcgtt tacagccgca ttaatcttaa agcttgaagc
     3901 ggaagggaaa ttaaacatca accaaacaat tggagattgg ttgccagaat atgatgactg
     3961 gaaaggcatt actataaagc aattacttaa tatgacaagt acaattccag gttatgcaac
     4021 gattccaacg cttgcagatt cgattactga taatcctaaa aaacagtgga ctagtaaaga
     4081 actggtagat ttagcatatg gatataaaca ccccacctca ggttataatt actctgatac
     4141 caactactta attctgggta tgattataga aaaagtcact cacgattcct ttcataatca
     4201 attacagaaa aaaattctaa agcccttaca attaaaagag acctattata ttactgattc
     4261 ttatacccca tattttttaa aaaaaatgcc ggaaagttac tattatggta gaacagtgga
     4321 aaacttaaaa catggtcaaa agtcaaccaa tgacaattta tcaatggcgg gacctgctgg
     4381 tgctattgtc gcaactacat cggatgtcgc caaatggata aaagctttat tttcatccga
     4441 ttttttactg ccgaaacagc aaatggagtt aaaatccctc atttcgatga aaacgggtac
     4501 tccaatctcc catgttgatt cagaagagcc taatggcttt ggtttaggtg ttgttcaagg
     4561 ctataagccg caattgggaa aatattggta ttatgaaggt gatacagtgg gatttagagt
     4621 cttatatatt tatcatcctt gtaatgaaaa aatagtggtt attgcattaa acagttcgcc
     4681 ttttgttgat gaagcagcgg gagatcatag tggtgatttg ttattggaat tgtacaaaaa
     4741 cgcttttggc atatcacaga aatgtgaata aaatagggta aaatccaact tcttttggca
     4801 acctttcaaa aagaggcgag tcatgcaaga gagatatagg ttatggcctt aaagtacttg
     4861 taaatccggt gcaaaaaacc tatttagata aaaaacgctt ctggaatacc tcgtgttttt
     4921 cattataaaa tgagttttta taattcgata aagtcgcttc attaaattaa ccggtacaac
     4981 ctcaaaatct ggagtaagta agttatgacc aatatcgatg ttcgctggca acaacggtta
     5041 aataattatg caagagcgct tcaacaatta tcacttgccg tcaacctggc gcaaacaaga
     5101 cccttatccg acttggaaaa gcaaggctta atccaagcct ttgaatttac ccatgagctg
     5161 gcttggaatg tgatgaaaga ttatttcttt ttccagggaa attctgcaat tactggttct
     5221 cgtgatgcaa cacgcgaatc atttaacaag ggattaatta aagaaggtga aatctggatg
     5281 gaaatgatta aaagtcgcaa ccaaacctct catacctaca accaatccgt tgccgatgaa
     5341 attgtcaaaa atattattaa tttctaccac acctcttttc aggcattcct ggaaaaaatg
     5401 caaggtctga aagagcatga atgagcaatt aaaccatctg tatggcttgc ccagccatgc
     5461 tattgaagca ttgaaatgtg tctttaaaga gtattctcaa attgataatg cgattctcta
     5521 cggatctcgt gccaaaggca catatcatca gggctcagat attgatcttt gccttaccgg
     5581 aaacttatta gggatcactg aattacttgc tattgaaaat aaaattgatg atttactgtt
     5641 accctggaag gttgatatat ccctgaaaca caccatagac aatcccgatt tgcttgaaca
     5701 cattgagcga gcgggtatcc tgttttatac aaaagaatcg taggacttta gggtctgttg
     5761 acatttcgct ggcttttcgg ccaagatagt agaaatgtca acagacccta ataaactatt
     5821 cactgcgtaa agaaaccatc ggctccagtt tcgaggcccg tcgggccgga taaaatccga
     5881 aaaatattcc agtggcagca gaaacgagaa agccggcaat tggggggagt aagtagatag
     5941 taaaagtcca gtcactgaaa taagcgacta ttctggtaaa aatcaaacct aaaatgactc
     6001 cgagcacacc ccctagcaaa gacagcatga cagattccac caaaaataag gcctggattt
     6061 ctctgttttt cgcacccact gctttgcgaa tcccaatttc ttttttgcgc tcgctgaccg
     6121 aaaccagcat cacattcatg acaccgatgc cgccaaccag caaggatatt ccaccaatga
     6181 ccgccaacag caaggtaaaa atacgaccct gattttccat gctggctata atttgtttgg
     6241 cgctgcgcgg aaaaacactg agcttcggcg cctgagaatt aatgatttcc tttatttcat
     6301 taatgacctc atcaatgggg ctgtccggct tgagaagcaa tactgcatta ttgattttgg
     6361 cgtccttgct gaccaggcta attccagcca ggggaataat gacagcctgg ttaatgtcct
     6421 cattaaaaaa accattttct ttccaacgct cggcaacgcc gataatggta tacaaggatt
     6481 ggcctatgcg taattgcttg ccaatgggat catccagggt aatttctttg atttgttgtg
     6541 ccaggctatc gccaataaca caaaaatgct caaaagaatc caaaaaagaa acaaaatgcc
     6601 cctgcgccaa ggtcacatga atgatatcag caaggctttc atcggcgcca atcaccgccc
     6661 cctgcatcac tttgccatta aagcttaaag tttggtaagc cgtgctgtaa ggcgcaattc
     6721 ttaagacata aggtatacgc tcggctatct gttgccattg atctattgtt aacgagtcgg
     6781 attcactatg ggatttgctg ggcacctttt gataaacaga aacagccagc aaatcagtac
     6841 ccaatgcctt gaattgctcc agcgccttct cggtagccag ctgtccacag ctaatcaagg
     6901 caacgacagc ggcggtccct accagtattc ccagcacagc caaaagcgat cggagcttgg
     6961 aggctgttaa attaaccaag gcttgttgac aatgattaag aaaattcatt gcccggactc
     7021 cgctataatt gctccatcgg ctaaggttat tcggcgtttg cattgcgctg ccacgtgctc
     7081 atcatgggtc accatgataa tagtccggcc ttcctggtgt aaggccaaaa acaaattcat
     7141 tacctcgcta cctgttcttg aatccagcgc accggtcggc tcatcagcga gaataacctg
     7201 tgggtcggtc actaacgctc tggcgatagc gactcgctgc tgctgaccgc cagataattg
     7261 ggttggcctg tggcggatgt attggcgcat tcctacttta tccagtgctt gctccacttt
     7321 ttcttttatc aaagtgggat tgactcctcg gtacgtcaaa ggcaaagcca cattttgcat
     7381 cgctgtaaaa cgaggaagca aattaaactg ctgaaaaaca aaacctatat tttgattgcg
     7441 caaatcagcc gcctcatcgt cgcttaaact ggccacattt ctattattta aggtataagt
     7501 tcctgcatca ggtttatcta ataagccaat gatgttcatt aacgtggatt taccagagcc
     7561 ggacgcaccg acaatagcga gcaaatcacc ttcgtttacg gtcaaagaca ctgcttttaa
     7621 taccgtggta ctgataccct caagatgata agtcttcacc aaatcagaaa gttggattaa
     7681 aggtctgtca atcataaacc acgacatctc ctgatttaag gccactttca accactactg
     7741 aatccgcttg agcggcaccg gtagtaatca ttcgtttctg aactttcccc ttggctaatt
     7801 ggacatgaac aacgctgctg cctttttccc ttttaaccgc cgcgataggt attaacaatt
     7861 gattattcga ttccacattc agctctatgg aggcactcat ccccactttg atccacgact
     7921 gttgttcagg agttagggaa ttgacctcca ctacagcggt aaaggagggt aatcctcccg
     7981 tgctggtatt cgatgcctgc gcattcaccg ccaccaattt acctttcagc tgatgcctgc
     8041 caaatgcaac gccactaatt gttgcctcca ttccggggcg aattttatca atgtctatct
     8101 ctgggacatc aatttccaca ctgattcctt tcaaatcgcc tatcaaggca atgacttgtc
     8161 ctgactttat ggtcgatcct acggtcactc gattgttttt atcctctcct gatttgggag
     8221 ggtacagcat tatcccatca ctgggagcct ttaaatgaat cagattatga tttgacgtaa
     8281 gaatcttcct caccttgtca aaatcagcca gactgagatt ggatatattc tgagtgtttt
     8341 tatcatccat tttttccaac atttcagaaa gcttgcgggt ggcctgcatt aaagtaaccc
     8401 gcgctgtatc caagcctgat ttttcactta agtaattgtt tttggagatt aacccggcat
     8461 tccacagctc ttcggttcct acaaattttg ccttggcaat ggaataactg tctttcgcct
     8521 ttaagtaatc cgttaatgtg tcgttatact gtttttgcaa ttcattggaa tttaaagtta
     8581 aaacaatctc tcccttttta accatctggc cataatggaa atgcatggtt tcaacaacgg
     8641 cttccattgg actggtcagg ctgctttccc ttatgggttg cacggtcccg gtaaaatgca
     8701 aggtcttgtg taaaggttga ggtttcactt catacgtttt taatgaagag gtttgcggct
     8761 tatcagaact gccacaagaa accagcatta aaatcaatga cgctatagag aatattttca
     8821 gataattgtt catccgccat accttagttt aatctgccag taatccagcg tggtccctaa
     8881 aatcctttgc aacgcggata actgattgag atacgcaatt tttgccccta tcaatcctga
     8941 ctgtgcctga atcaattgat tctgagtatt gttcacatcc agggcgctgg atattcctgc
     9001 ctgctgtttt ttcttttcca aagcgtaaga ctgctctgcc aatttgactt gcttttgtgc
     9061 cagctcatag cgcttggcca agctttgaat attattgatg gtattggtta cactggtaat
     9121 taaagctctt ttggctgcga taagattgat tttgtctttt tccaactgga ctttagcatt
     9181 aattaattga ctacgccgtc caatgtcatt caatggaatg gtcagggtga ccctggctga
     9241 ttccgtgata ttattcccat tgtaaatgct gcttaaacca ctgctggttc cggtgacatc
     9301 gttgactgcg ccgccttgca cagtacccgc caaatcaagc tgccaaagtt gctgattttt
     9361 tgcaacagtg taagacctct catccgcacg caaagccatt ttctgggcca gatactgagt
     9421 attgtgactt aatgcgatgg caatggattc ttgcaagtca ggcactataa tttcctttat
     9481 cgtcacatca ctgggaactg aaatatgcaa atccggatca agcccaatca tttgcaataa
     9541 ttcctgagat gctaatttaa aatcattttc accttgttct accatcaaac tcaaagattc
     9601 tatctgatag gactgctgaa tatttccggt aggttctaat tgtcccgctg caatcttttt
     9661 ttcattaata cgataggatt ttctggcttc ttccaattgc aagcgctgat tctctaaatt
     9721 attcccactt aaaatcaaag ccctgtacgc cgtaatcacc tgggtaatct gatccataac
     9781 cgattgctgt aaacttaatt tatttagcca ttccttatca atcgcatcca gcagttttgc
     9841 ttcattcgct gacttgccaa atccacggag caaaggctga gtaatggata aattcaatac
     9901 cggattatag ctgttgttgg tggaaacatt gttatcgatg gatacggacg ccttggtgcc
     9961 tattttggtt ttcaaatcca attcaggact ggccagaaaa gaatggttcg tagaactgcc
    10021 tatcccctca aaagtacttt tttgcacaac gcctgaagca ccaagagcat attgcaattc
    10081 aaactcatta ttagccaaac gcaattgata gcgctgaata atacggtcta attccgcatt
    10141 ctggatattg ggattataac gcaatgccaa cagtatagct tctcttatcg tcaactgatg
    10201 tcttgttttc ttttttgatt caggcgaggt aggcaactct atccaatttt tcagcatgta
    10261 ttccgggttg gcaatggctg cctttagtct ttcctccgct tgctctactc tctgagcttc
    10321 agctgaggga ttttctgtcc aggaataaag tgaattatca gcatttgaag aagtcttggc
    10381 acttgaggca tgaaaactaa tcaggcccga taaaataatt accggtaaca atgctgtgct
    10441 tttatttaaa aatttcaccg ttgttatcgc ctgtgtttat aaaaatgtct atccatcaag
    10501 gttattcaaa acgactaacc ccatacccgg gtaccgaaaa gcgttgcctg tcctgcaggc
    10561 gtaatgccgt taattgccca ccccaaagac atccggtatc tatcgcatga atccgcggat
    10621 gagaacttcg ccccatgagc gccgcccaat gaccaaaaac gatatccatt tcaatttctt
    10681 ttcggcatgg cacttcaaac cagggataca gatgattcgg ggctttggat aaagtcccct
    10741 tataacctaa atctaatcgt ccatgggcat cacaatagcg catccgggta aaataattgg
    10801 taatgactcg taatctatcc agcccaacca aatcatctga ccaaatatcc ggcttattgc
    10861 cgtacatttg ggcgaaaaat tcgtgatatg aatcgcctga aagcaccgct tccaattcat
    10921 tagccaggtc cacggccttc gataaatccc ataaaggcgc gatacctgca tgacacatga
    10981 ctatgtttaa ctcagatgac ctgcaaagaa gggattgctt gcgcaaccaa tgccccagct
    11041 cctctccatc atcagccagc atcacctctt caagtgtatc atcatgccct ttccagggtt
    11101 gtccgccgaa taataagcct aataaatgca aatcatgatt acccaaggtt attttgggtt
    11161 tcacaggcaa ggaataaata aaccgtaata cagccaatga atcaggaccg cggtttacca
    11221 agtccccaac aaaccacaag caatcttcct tctcgttaaa atcaattagc tcaagcaacc
    11281 gttgtaaagg atcataacaa ccttgtacat cccctattgc ataatcaggc accgcgtttt
    11341 gctccaagtg tcaattggga catatcctgc tcaatattct gccattggcc attggctgcc
    11401 aatcgctgca aaggcgctat tgcaatcgca cttgattgct gtgtgaattg gctggtgtat
    11461 tgtgatttat gaattaactc cagtgctgac atggactcta ttttacccgt attcaaatga
    11521 tgcacctgga caaagttggg caaagcaacc ccatatcctg caaaaacgac cccggtatac
    11581 atttgctgag tatgagcctg ttcaaaacga ctggttacat tgccccccgc atcccggcca
    11641 tgaagcccta atgacaaaaa cccattctgt atggcttgcc aatttttata ttcaggatgc
    11701 tcacgaacaa acaattgaaa catttcaggg cacatcagca tgccgccagg aaccataaac
    11761 aacggggctt tgttcaccat aattcgacct tcatccaacg acttgataat ccattggatg
    11821 aattcgaccc ctattgcctg ttccttttct gttatagatt ccggtacacc gccggtaaaa
    11881 gttacagctc tgccataacg gccgcttcgt cttgccccac ttcgtgccat aaacgccaga
    11941 aaatagcgct gtatcgcaat ggcatcagcc cgaattaaaa tagcgcccaa agtaccagca
    12001 gaacgataat cttcattcag taaagctaac caaacagcca atacttccgg attggaggca
    12061 atccaggcaa aaccactggc cggcatcaaa gttctggcca gcaacaagtt cagccggcag
    12121 cggaactcca cgtccgcttc tttctcaaac tgataatcat aataacttcc agtttggatt
    12181 aagctttcta atagagggtt ccattgtttt aacaattgac cgttgctatc aaacaaatta
    12241 atgcgaaact catcaaataa tttgccaagg ccttgtaaaa ttgccgctga ataaagagca
    12301 tattgccaaa gcttctgttc ctcggaaagc aaatctggct gttcttgaac ggtaaactcc
    12361 ttgaatagac tcaatgcggc ttctgttctg tttaaagcat gatcgaccaa accacctggc
    12421 tgggaataat aactgttcgc ggtttccggc aaattttgac aatagttcac cagattctcg
    12481 acaagaacaa aacataaact ctcatagcga gatgcttcca acccgcttaa tgccctcatt
    12541 ttctggagta atacctggcg tttctcttca ctcagaaact gctctgcggt caccatgcgg
    12601 attaaatctt ttaatgactt ggactgagca gataaagagc ttttacgctg acgatgaaac
    12661 aagacaacct ccacaataac ttattatata attttacact aattggaaat aaatttcgct
    12721 agttggacat attccgccac cgaaatttgt tctggccgct tacctccatc aatgcctaaa
    12781 tcattgagtt gagataatga gataatccct ttcaaattat tagtaagagt tttgcgtctc
    12841 atagcaaatg ctttagcaac aatattttcc agtttctcag tgtttacaga ctcaaatgga
    12901 gaaacacgat gaggtgttaa tcgaactatt gctgagtcta ccttgggcct aggctcaaac
    12961 gcttctggag gaacatcaaa taaatactct acctcacaat gatactgcaa catcacgctt
    13021 agtcgcccat aggctttagt gccgtgggct gctgctattc gttctacaac ttctttttgt
    13081 aacataaaa
//
LOCUS       DASLOE010000081        13041 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.081, whole genome shotgun sequence.
ACCESSION   DASLOE010000081 DASLOE010000000
VERSION     DASLOE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 13041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13041
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.081"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(54..1166)
                     /locus_tag="U6309_001771"
     CDS             complement(54..1166)
                     /locus_tag="U6309_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEN5505211.1"
                     /translation="MNIFNLPDLGEGLPDAEIHEWFVKEGDTVKADQPLVSMETAKAV
                     VDVPCPQSGTIAKLYGNPGDVIKTGEPLVAFVSTTEKPADKGTVVGNLEESTDVLEDN
                     FIIGSQRSSHRVKTTPAVRMLAKKLGVDLSSLKGSGDNGVITREDVQNQANINSQPPA
                     GFEPLRGVRRAMLNSMVQSHAEIVPVSIFDEADIHIWKPNTDITVRLIRAIIHASKKE
                     PALNAWFDTKHAARRCFKEVHLGIAMDNEEGLFVPVIHDAEKYSDSELRKIIDEFKES
                     VRNRAVSADKLKGATITLSNFGKFSGRFASPIIVPPMVAILAVGRLYKAAVINDEDKI
                     EAHKMLPLSLSFDHRAITGGEATRFLGAVIESLQQA"
     gene            complement(1169..2143)
                     /locus_tag="U6309_001772"
     CDS             complement(1169..2143)
                     /locus_tag="U6309_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEN5505212.1"
                     /translation="MPDITLVEAVTQALAYELAHDENVVVFGEDVGKNGGVFRATVGL
                     QDRFGENRVFDTPLAESMIAGLAVGMSIQGLKPVAEFQFMGFIYPAMNQIISHAARMR
                     NRTRGRLHCPLVYRAPFGGGIRAPEHHSESTEALFAHIPGLQVVIPSSPKRAYGLLLA
                     AIRNPDPVIFLEPKRIYRLVKQPVPDDGQALPLGKCFTLQQGDDLTLISWGASMHETL
                     QAAKQLTDEGISCDVIDVATIKPLDIETILSSVEKTGRCVIVHEGAKTCGVGAEISAQ
                     IMEHCMADLLAPVQRVTGYDTVIPYFQLEKQYIPSIARIKNTVMSIME"
     gene            complement(2136..3209)
                     /gene="pdhA"
                     /locus_tag="U6309_001773"
     CDS             complement(2136..3209)
                     /gene="pdhA"
                     /locus_tag="U6309_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEN5505213.1"
                     /translation="MTTVAQFEITYTQYLNEQGKLVAELPPFADNQSILKELYKIMVL
                     TRTFDKKAIALQRTGKMGTYAPINGQEAISTAIGHALKSEDVLVPYYRDYAAQFQRGV
                     KMSEILAFWGGDERGSHYSNNAEDLPICVPIASQCLHAAGVAFAFKYRNQPRVAVVCI
                     GEGGTSEGDFYEAMNVAGAWRLPVVFVVNNNKWAISVPIEKQTGSQTIAQKAIAAGFS
                     GVQIDGNDIFAARQVIGDAIEKARQGGGPTLIEALTYRLCDHTTADDATRYQPSHEVE
                     EAKAKEPIVRFKHYLMQQNIWTSQDEEKLVIECSETVEKAVEEYLNTKPQPVSSIFDY
                     HYAELPEYLIEQRAIAMEEYSHA"
     gene            3390..4712
                     /gene="pabB"
                     /locus_tag="U6309_001774"
     CDS             3390..4712
                     /gene="pabB"
                     /locus_tag="U6309_001774"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEN5505214.1"
                     /translation="MIDSTLITLDYHDFSSESYEKFSRLPGFVLLQSTDKSMGRYDIL
                     SAYPYERMVIKNNIHDKNLVLTELKNKLKIEQSELDLPFQGGAIGYVSYDFGTLLHNH
                     EQYPQKDLAHIPVLNFGFYDWAIIVDHHLKKVTLFARNSHSSTKSVVDEIKNILNNQP
                     AKSVQFSVQAEFLPLISKENYRSSFNAIQQALRAGRSYQVNFTQPFQSKFTGDPWYMY
                     QRVNRNNFVPYAAFIRTEEADILSFSPERFLLYDNGEMLTSPIKGTIHRSKDPVVDKK
                     LKEQLLKCPKNKAENVMIVDLLRNDLGKLAKAGSVKVMNLCHIQSFNAVHHLVSDIRA
                     LCKEDIHPIEAFFSCFPGGSITGAPKLETMKIISEQEIYKRGVYCGCIAYFSQHGRFD
                     ANIAIRTVTAKDNVLYLAAGGGIVIDSDWEDEYRECFTKITAIINGIK"
     gene            4699..5202
                     /locus_tag="U6309_001775"
     CDS             4699..5202
                     /locus_tag="U6309_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEN5505215.1"
                     /translation="MESNNTQQSIKTHSSVIVLYDLSKDSLILTKRSQMLNKHPGEVC
                     FPGGFQEINDQDLYSTALRELNEELGVTSDRITLIRKLNIEKTLLGLVIHPWYASIDS
                     IFPYTMNPQEVSKLILVPMPFVKNQKNYKEIRIEKEGKHFVTCQFIANEELIWGATAR
                     IMRQLAS"
     gene            5363..6079
                     /locus_tag="U6309_001776"
     CDS             5363..6079
                     /locus_tag="U6309_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN5505216.1"
                     /translation="MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLHG
                     FDIDLMQEICRRLHATCAFEAYIFDDLFPALKNRKVDLVIASMIITDERKKLFIFSLP
                     YMESNSQYITTIHSKINTFDDLQDKKIGVRKGTPYARQVLSANRNNQVIFYELIQDML
                     LGLSNNEVDASLMDYEAAKYWMASEPYAYKLIGNKISIGEGYSIMANPDQYVLIKKIN
                     KILLEMEADGTYLRLYSEYF"
     gene            complement(6127..7836)
                     /locus_tag="U6309_001777"
     CDS             complement(6127..7836)
                     /locus_tag="U6309_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEN5505217.1"
                     /translation="MDKRWFEQYQNGVPHEIDASQYLSLISLFNESCNSYASRAAYSN
                     LGTEITYAELDSLSRNFAAYLQQLNLEKGARVAIMLPNVLQYPVAIFGVLRAGYVVVN
                     TNPLYTADEVIHQMNDSGAEVIIVLANFAKTIEKALPSMPSVKHVIVTQLGDLFPSFK
                     RFIVNFVVNHIKKLVPAFTIPHAVAFNYALSEGKQATLHRVELNHDDIAFLQYTGGTT
                     GVAKGAILTHGNLIANVMQAYTWISPLGVSNQDVIVTALPLYHIFSLTANCLTFMKIG
                     AKNILITNPRDMGHFIDQIKNSGFTAITGVNTLFNALLNHPKFKEIDFSKLKLALSGG
                     MALQKSVSLKWSEMTKTRVLEAYGLTETSPAVTINPMYLEGYNGSIGLPLPSTDVSIR
                     DESGKEVPIGTSGELCIKGPQVMPGYWKRPDETALVFTKDGFLKTGDIARMDEEGFIY
                     LVDRKKDMIVVSGFNVYPNEVEQIIGMHPGVLEVGVVGIVDEESGERVKACIVRKDST
                     LTAEQIINHCKEHLTAYKIPKVVEFFDELPKTNVGKILRRALKDQEKEQKYSVNKKTM
                     EPV"
     gene            7908..8603
                     /gene="minC"
                     /locus_tag="U6309_001778"
     CDS             7908..8603
                     /gene="minC"
                     /locus_tag="U6309_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEN5505218.1"
                     /translation="MGENITNTQAFKLKGRLYTLTVLQVLNTDKDAFSQQFAEIVVRA
                     PKLFERTPVVFDLSSVNHLEFDLQELCQIARNHGMIPLAIQGGSPLHDTLAQCQGLGI
                     LNASTAHDKPIIEEPSECQSIENFKTKLLTTPVRSGQQVVAKGADLIVTSSVSHGAEL
                     LADGNIHVYGTLRGRALAGISGDIEARIFCQSLDAELVSIAGFYRISDAIEPVNGPCQ
                     IYLANEHIVVEPL"
     gene            8600..9328
                     /locus_tag="U6309_001779"
     CDS             8600..9328
                     /locus_tag="U6309_001779"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEN5505219.1"
                     /translation="MIDSVFISDLHLHPEDNEIQTRFDHFVNWARLSVKRIYILGDFF
                     HAWSGDDSINAWSTAIAEQIESLTQSGIEIYFMTGNRDFLLGSLFAKRAGWKILTEPT
                     IITLGNHKILLVHGDRYCTNDRSHQRFRLLTRNRWFSFLFLRLPLSFRKRLVNGVRKI
                     SLKNHHKSIEQMDIVTEAAIRHMSENNTEILIHGHTHKPGITNYTYEEKILTRYVLSD
                     WDDIPKLLCYDNTKGFYFTQYDIN"
     gene            9325..10071
                     /gene="ravY"
                     /locus_tag="U6309_001780"
     CDS             9325..10071
                     /gene="ravY"
                     /locus_tag="U6309_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavY"
                     /protein_id="HEN5505220.1"
                     /translation="MRSYPMSKPSNKMKEELEQIRKTEIENAIAKEREDLLAQRSRER
                     AEADDNLKKGRYIKDPRTGQSITLWESAVQKAEEVLNADLNSYMDARAAIMSLLKMYY
                     EMVKANAQTMKELRAGIGNTIMDWGVFPIKDYIGKKLTGNPEIDLPILQHEVSYTDDN
                     KLKIEPLTRSDKREEKGQLDKLFEGMIHMWLKNNDYTPDRDNPGQFVNSHGETLDKAT
                     FDKLKKDDQHGLNHFLTEYDPDLQFRPSRP"
     gene            complement(10160..10783)
                     /locus_tag="U6309_001781"
     CDS             complement(10160..10783)
                     /locus_tag="U6309_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HEN5505221.1"
                     /translation="MLKRLDSDLQTLNDFLKTKTPEAWLNHAAANIPLLLLDHAHCER
                     KAAGTAINFISKYPEKAELVAIMAPLAREELLHFEKVIDIMKQKGIVYSPLQPSDYAS
                     NLHKHVTNKDGIERLCDQLIIGAIIEARSCERFSSIIPYIEDQDLARFYKTLVKSECR
                     HFEEYLHLAKFYGGSVDDRISEFLTIENAFILSDDVVFRFHSGLPPA"
     gene            10857..11741
                     /locus_tag="U6309_001782"
     CDS             10857..11741
                     /locus_tag="U6309_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505222.1"
                     /translation="MDEIKKDDELSQWLSTYGTITAERILGRYNISLPQDEILEAINI
                     PSSFYRHLLQIPLKNVLNGIVIQQASDYHVYAQKLLIDYLLSGESSKEPDSQGAGTRE
                     SLEDERQRLVQLGDEFHKLELEQDNLIASSQASLMKISIDWNTKLETTLSKLNSLYKN
                     TNSKIKKNAIRKALIKAFIHCDLVKDQSQKNKHQLIDKLNQTLAVSVGAELKESILTN
                     LGELFQILGALNTKLDEFTDRTNHLSQKAKSFRTQFYEVILRIIELIKLLPEYKIDPE
                     QDAINREPLYFDRTIGER"
     gene            11876..12301
                     /gene="ndk"
                     /locus_tag="U6309_001783"
     CDS             11876..12301
                     /gene="ndk"
                     /locus_tag="U6309_001783"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEN5505223.1"
                     /translation="MAKELTLSIIKPDAVAKSVIGEIYTRFEKAGLDIVAAKMTQLSR
                     EQAESFYDIHRARPFFKDLVDFMISGPVMIQVLKGENAVAKNREIMGATNPKEAAPGT
                     IRADFADSIDANAVHGSDSLENAAREIAFFFEPHELCNR"
     gene            12322..>13041
                     /gene="rlmN"
                     /locus_tag="U6309_001784"
     CDS             12322..>13041
                     /gene="rlmN"
                     /locus_tag="U6309_001784"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN5505224.1"
                     /translation="MDQQKVNLLNYNYSQLRELLIAWDEKPFRAQQLFQWIHQVGIRD
                     FAQMTNLGKVLRNKLSQLACIDLPEIVACQKSADGTHKWLLKLECGNCIETVFIPEAN
                     RGTLCVSSQVGCALNCSFCSTAKQGFNRNLSTAEIIGQVWLAARELSDNNGTHDKKIT
                     NVVMMGMGEPLLNFDNVVSAMNIMMDDLAYGLSKRRVTLSTSGVLPEMERLREVSPVA
                     LAVSLHAPTDELRNELVPINKK"
BASE COUNT         4224 a         2453 c         2380 g         3984 t
ORIGIN      
        1 aatagtgttt gttgttaggc ttcgctattt acgaagccca acataagtta aaatcatgct
       61 tgttgcaaag attcaataac agcacctaaa aatcgagtgg cttctcctcc agtaatagct
      121 ctatgatcaa agctcaagga taaaggaagc attttgtggg cttcaatttt atcctcatca
      181 ttaattactg cggctttata aagtcttcca accgctaaaa tcgctaccat tggaggaaca
      241 attattggac tggcaaaacg tcctgagaat tttccaaaat tagacaacgt aattgtcgcc
      301 ccttttagct tgtctgcact aaccgcccga ttacgaactg actctttgaa ttcatcaatt
      361 atttttctta attcactgtc ggagtatttt tctgcatcat ggataacagg aacaaataaa
      421 ccttcctcat tgtccatggc aatgccaaga tgcacttctt taaaacatct acgtgctgca
      481 tgtttggtat caaaccaggc attaagagca ggttcctttt ttgacgcatg gataatggcc
      541 cggattaaac gaacagtaat gtctgtgttg ggtttccaaa tatggatatc tgcttcatca
      601 aaaatactga ctggaactat ttctgcatga gattgtacca tgctattaag catggctcgc
      661 ctaacacctc gcaagggctc aaacccggca ggtggttgtg aattgatgtt ggcttgattt
      721 tgaacatctt ctcgagtaat aacaccattg tcaccactac cttttaaaga gcttaaatca
      781 actcctaatt tttttgctaa cattcttaca gcaggtgttg tcttaacgcg gtgagaagaa
      841 cgctgacttc caataataaa attatcttct aacacatcag tactttcctc cagattacca
      901 acaactgtgc ctttatctgc aggtttttcg gtagttgaaa caaacgctac aagaggctct
      961 ccagttttga taacgtcacc tgggttgcca taaagttttg cgatagttcc tgattgaggg
     1021 cagggtacgt caacaactgc ttttgccgtt tccatagaaa ctaatggttg atctgcttta
     1081 actgtgtcgc cttccttaac aaaccattca tgaatttcag cgtcaggtaa accttcacct
     1141 aaatcgggta gattaaaaat attcattatt actccattat actcataaca gtgtttttaa
     1201 tacgtgcaat gctaggtatg tactgttttt caagttggaa ataagggata accgtatcat
     1261 atcccgtaac tctttgcaca ggagctagta aatctgccat acaatgttcc ataatttgag
     1321 ccgatatttc agcacccact ccacaggttt ttgctccttc atgcactatg acgcaacgcc
     1381 ctgttttttc tacagaactt aaaatagttt ctatatccag aggcttaatt gttgctacat
     1441 caataacatc gcaggaaata ccttcatcag ttaattgttt ggctgcctgc aaggtttcat
     1501 gcatactggc tccccagcta attaatgtca gatcatctcc ctgttgcagg gtaaaacatt
     1561 tacctaacgg taaggcttgt ccatcatctg gaacaggttg tttgaccaat cgataaattc
     1621 gttttggttc aagaaagata acgggatcag ggttacgtat agctgccaac aacaggccat
     1681 aggcgcgttt tggagaagag ggaataacca cttgcaaacc cggaatatga gcaaataatg
     1741 cttctgtact ttcagaatga tgctccggtg ctctgattcc accaccaaac ggagcacgat
     1801 aaaccaaagg gcaatgtaag cggcctctgg ttctgttacg catacgcgct gcatgagata
     1861 tgatctgatt catggccgga taaataaaac ccatgaattg aaattcagca acaggtttta
     1921 agccttgaat agacatccct acagcaaggc ctgctatcat cgattctgct aatggagtat
     1981 caaaaactct attttctccg aatctgtctt gtaaacctac agtagctcga aatactccac
     2041 catttttacc aacatcctcg ccaaagacta ctacattttc atcatgagct aactcatacg
     2101 ccaaagcctg agtaacagct tcaactaaag taatatcagg catgagaata ctcctccatg
     2161 gctattgcgc gttgttcaat taaatattct ggtaattctg cataatgata atcaaaaatg
     2221 ctgctaacag gctgaggttt ggtattcaaa tattcctcta ctgctttttc taccgtttca
     2281 ctgcattcta taaccagctt ttcttcatcc tgagatgtcc aaatattttg ttgcattaaa
     2341 tagtgtttaa aacgaacaat aggctctttg gcttttgcct cctctacttc atgagaaggc
     2401 tgatagcgtg ttgcatcatc agccgttgta tggtcgcaca agcgataggt taaggcttcg
     2461 attagagtag gtccaccacc ttgacgagcc ttttcaatag catcccctat aacttgtctt
     2521 gcagcaaaaa tatcattacc gtctatttga acgccagaaa aaccagcagc aatcgctttt
     2581 tgggctattg tttgtgagcc agtttgtttt tctataggca cagaaatcgc ccatttgtta
     2641 ttatttacta cgaagaccac tggtaatctc catgccccag ccacattcat agcttcgtaa
     2701 aaatcacctt ctgaagtacc tccttctcca atacatacca cagctactct tggttggttt
     2761 ctgtatttga aggcaaatgc gactcctgcg gcatgtaaac attgagatgc aattgggaca
     2821 caaataggca aatcttcggc attattagaa taatgacttc ctcgctcatc ccctccccaa
     2881 aaggcgagta tctctgacat tttaacgcct ctctgaaatt gagcagcata atctctgtaa
     2941 taagggacaa gtacatcttc agatttaaga gcatggccaa ttgcagtcga aatagcttcc
     3001 tggccattaa ttggcgcata ggttcccatt ttccctgttc tttgcaaagc aattgctttt
     3061 ttatcaaaag tacgtgtgag taccatgatt ttatacaatt ctttaagtat ggattgatta
     3121 tctgcaaatg gaggtaattc agcgaccaac ttaccttgtt cattcaggta ttgtgtgtat
     3181 gttatttcaa attgagcaac agttgtcatt acatggctcc ttgtcaacaa cgcgttggca
     3241 taggatactc acaaattaat ttaaaaacca gtaaattaat aggaacccaa gataaaaata
     3301 tacgtttttt attgataagt taggttaaaa tgaccaaatg taaaacactg catttttttc
     3361 actgcacatc cttatcttga ggtgataatt tgattgatag tactcttatt acacttgact
     3421 accatgattt ttcatctgaa tcctatgaaa aattcagtcg tttacctgga tttgtcctgc
     3481 ttcagagtac ggacaaatcg atggggcgtt atgatatttt aagtgcttat ccatatgaga
     3541 ggatggttat taagaacaat atacatgata aaaatttagt attaacagaa ttaaaaaata
     3601 aactaaaaat agaacagtca gaacttgatc ttccttttca gggcggggcc ataggctacg
     3661 tttcatacga ttttggtaca ctattacata atcatgaaca atatccccaa aaagatctgg
     3721 cgcatatacc tgttttaaac ttcggatttt atgattgggc tatcatagtt gatcaccatt
     3781 taaaaaaggt cactttattt gcaagaaaca gtcattcttc aaccaaaagc gttgtagatg
     3841 aaataaagaa tatattgaat aatcaacccg caaaatcggt acaattttca gttcaagctg
     3901 aatttttgcc tttaatatca aaagagaact ataggagctc attcaatgcc attcaacaag
     3961 ccctgagagc agggcgctct tatcaggtta atttcactca accgtttcag tctaaattca
     4021 ccggagatcc ctggtatatg tatcaaaggg ttaacagaaa caactttgtc ccctatgctg
     4081 cttttattag aactgaggaa gctgacatat taagcttttc tcctgagcga tttttacttt
     4141 atgacaacgg tgagatgtta acttcaccca taaaaggcac tatccaccga tcgaaagatc
     4201 ctgttgtaga taaaaaatta aaagaacagt tattgaaatg ccctaaaaac aaagctgaaa
     4261 atgtcatgat tgtcgatttg ctaagaaacg atctggggaa acttgccaag gctggcagtg
     4321 taaaggtaat gaatctttgc cacatccaga gtttcaatgc agtacatcat ttggtttccg
     4381 acataagagc tctatgtaaa gaggatattc atccaattga agcatttttc tcctgttttc
     4441 caggtggttc aattaccggg gcgcctaaat tagagacgat gaaaattatc agtgaacagg
     4501 aaatctataa aagaggggtc tattgtggtt gtatagccta tttttcacag catggccgtt
     4561 tcgatgccaa tatcgcgata cgtacggtga cagccaagga taatgttttg tatttggcag
     4621 caggaggggg aatagttatt gactccgatt gggaagatga atatcgtgaa tgtttcacaa
     4681 aaattactgc aattatcaat ggaatcaaat aatacccaac agtcaatcaa aacacattca
     4741 tcagttatcg ttttatatga tctttcgaaa gactcgttaa ttctcaccaa gcgaagtcag
     4801 atgcttaata aacacccagg agaagtgtgc tttcctggtg gatttcaaga aataaatgat
     4861 caagatctat atagcactgc attgagagaa cttaatgaag agttaggtgt tacatcagac
     4921 agaataactc tgatcagaaa attaaacatt gaaaagactt tactgggttt agtcattcac
     4981 ccttggtacg ccagtattga ttctatattt ccttacacta tgaatccaca agaggttagt
     5041 aaattaattc ttgtgcctat gccttttgta aaaaatcaga aaaattataa agaaattaga
     5101 attgagaaag aaggaaaaca ctttgtcacc tgccaattta ttgctaatga agaattaata
     5161 tggggtgcta cagcaagaat tatgaggcaa ttagcttctt aatattaagt atgataccag
     5221 gacaataaaa ggcttttctt taaatgcctt tacctgatta ttaaaccaaa ttaaagaaat
     5281 catatgctac aaattgtaca aatcaaattt tttgtaccat gattatgttt aaacaaactt
     5341 cctaaattac aggatatagt ttatgaagtt actacaactt ctcttattta ttattatttc
     5401 ctctaattct tattcattaa atttaacaat tggtacttca aaatttaatc caccttttga
     5461 agtttggtct ggcaacaaca gttcattgca tggatttgat atagatctta tgcaagaaat
     5521 atgtagaaga ttacatgcga cctgtgcttt tgaagcctac atctttgatg atttatttcc
     5581 tgcattaaaa aaccgtaagg tcgatctggt aattgcttca atgattatta cagatgaaag
     5641 aaagaaactc tttattttta gtcttcctta tatggaaagc aactcacaat atatcactac
     5701 gatccattct aaaatcaata catttgatga tctgcaggac aaaaaaattg gagtaagaaa
     5761 aggaacccct tatgcaaggc aagttttgtc agcaaaccgc aacaaccagg taatttttta
     5821 tgaacttata caagatatgc ttcttggctt aagcaataat gaagttgatg ccagtttaat
     5881 ggattatgag gcagcaaaat attggatggc atctgaaccc tatgcttata aattaattgg
     5941 taacaaaatt tctattggag aagggtattc tattatggca aaccctgatc aatatgtatt
     6001 gattaaaaaa ataaataaaa ttcttcttga aatggaagct gatggaactt atctgaggct
     6061 atattcagaa tacttttaga agaggagcat taaggacaaa tagcacccaa aatactccaa
     6121 ttacacctaa acaggctcca ttgttttttt attgaccgag tatttttgtt ccttttcttg
     6181 gtcttttaaa gcacgacgta aaatcttgcc cacatttgtt ttaggaagtt cgtcaaagaa
     6241 ttcgacaact ttggggattt tataagcggt caaatgctcc ttgcaatgat tgataatttg
     6301 ttcagccgtc aatgtactat cttttcttac aatacaggct ttaacccgtt caccagattc
     6361 ttcatcaaca atacctacaa cgcctacttc taatacaccg ggatgcattc ctattatttg
     6421 ttctacctca tttggataaa cattgaaacc tgaaacaaca atcatgtctt tttttctatc
     6481 gaccaggtag ataaagcctt cttcatccat acgtgcaata tcgccagttt ttaagaaccc
     6541 gtctttagta aataccaagg ctgtctcatc aggccttttc caataaccag gcatcacttg
     6601 tggtccttta atgcacaact ccccacttgt accaataggc acttctttcc cggattcatc
     6661 acgaatagat acatcagttg aaggaagagg taagccaata ctgccattat agccttcaag
     6721 gtacattggg tttatagtta cggcagggct agtctcggtt aatccataag cttccaaaac
     6781 acgtgtttta gtcatttcag accattttaa ggacacactc ttttgcaatg ccataccacc
     6841 tgataatgcc agttttaatt tgctaaaatc aatttcttta aatttaggat ggttcagcaa
     6901 tgcattaaat agagtattaa ctccagttat tgctgtaaaa cccgaatttt tgatttgatc
     6961 tataaagtgt cccatatcac gtggattggt gatcagaata tttttcgcgc caattttcat
     7021 aaaagtgagg caattggccg tcaaggaaaa aatatggtac aaaggaagtg cagttactat
     7081 aacatcttga ttgctgactc ccagaggaga tatccaggtg tatgcttgca tcacattggc
     7141 tataagattg ccatgagtca aaatagcgcc ttttgcaaca ccagtagtac cgccagtata
     7201 ttgtagaaac gcaatatcat catgattcag ttcaactcga tggagcgttg cctgtttccc
     7261 ttcagataaa gcatagttaa aagccacggc gtgaggaatt gtgaaggcag gaactaattt
     7321 ttttatatga ttcactacaa aattaacaat gaatctcttg aatgaaggaa ataaatcgcc
     7381 caattgcgta acaatcacat gcttaacgga aggcattgac ggtaatgctt tttcaatcgt
     7441 ttttgcaaaa ttagccagca cgataatgac ctctgccccc gagtcattca tttggtgtat
     7501 tacttcatcc gctgtgtata aagggttagt gtttaccacg acataacctg cacgtaacac
     7561 accaaagatg gctacaggat attgtaaaac gttaggcagc attattgcta ctcttgcccc
     7621 tttttccaaa ttcaattgct gcagataagc agcgaaattt ctgcttaaac tgtccagttc
     7681 agcataagta atttccgtgc ctaaattgga ataggcagct ctgctggcat aagagttaca
     7741 tgattcatta aataaagata tgagggataa atattgactt gcgtcaattt catgtggtac
     7801 tccattttgg tattgttcaa accatcgttt atccactttg atatccttct gttattgttt
     7861 tcctcatgat agtataaaca aaaaactctc taatggatat catgctaatg ggtgaaaata
     7921 taactaatac acaagcattt aagcttaagg gaaggttgta tactttaacc gttttgcaag
     7981 ttttgaacac tgataaagat gctttttctc aacaatttgc tgaaattgtt gttagagcgc
     8041 ctaaattatt tgaaagaaca cccgttgttt ttgacttgtc ttctgtcaat catctcgaat
     8101 ttgatttaca ggaattatgt caaattgcac gtaatcatgg tatgatccct ttggcaattc
     8161 aaggtggtag tcctttacat gatactctgg ctcaatgtca gggtttgggc atactcaatg
     8221 cttcaacagc acatgataag ccaataatcg aagaaccctc agaatgtcaa agtatagaga
     8281 atttcaaaac aaaattacta acgacaccag tgcgttcagg ccaacaagtc gttgccaagg
     8341 gagctgattt gatagtgact tcttcagtta gccatggggc agaattatta gctgatggaa
     8401 atattcatgt gtatggaaca ttacgcggca gagcattagc aggaatatct ggggatatcg
     8461 aggcaagaat tttttgccaa tctttggatg ctgaattagt atccatagca ggtttctatc
     8521 gaataagtga tgctattgaa ccagttaatg ggccttgtca gatctacttg gcaaatgaac
     8581 atatcgttgt tgaaccatta tgattgactc cgtttttata tccgatttac atctgcatcc
     8641 agaagataat gaaattcaaa ccagatttga tcattttgta aattgggctc ggctatctgt
     8701 aaaacggata tacatactag gtgatttttt tcatgcctgg tcaggtgatg attcaattaa
     8761 cgcatggagt actgctattg cagaacaaat agaatcattg actcaaagtg gcattgaaat
     8821 ttattttatg actggaaatc gtgatttttt acttggttca ctatttgcca aacgagcggg
     8881 ttggaaaata ttaacagagc caaccattat tacacttgga aatcataaga tcttgctggt
     8941 tcatggcgat cgttattgta ctaatgacag atcacatcaa cgattccgat tattaacgag
     9001 aaacaggtgg ttctcatttc tttttttaag acttccttta tcatttcgca aacgattggt
     9061 taatggagtt cgcaagatca gtctaaaaaa tcaccataaa tcaatcgaac agatggatat
     9121 tgttacagag gctgcaattc gacacatgtc tgaaaataat acagaaatac tgattcatgg
     9181 acacacgcat aaacctggca taacaaatta cacttatgaa gaaaaaatac tgacaaggta
     9241 tgttctaagt gattgggatg acatacccaa actattgtgt tatgataata caaaaggatt
     9301 ctatttcacc caatatgata taaattgagg agttacccaa tgtctaaacc atcaaataaa
     9361 atgaaagagg aactcgaaca aattcgaaaa actgaaatag aaaatgctat agcaaaagag
     9421 cgtgaagatt tacttgccca aagaagccga gaacgcgctg aggccgatga caacctaaaa
     9481 aaaggccgat atatcaaaga tcctcgtaca ggccaatcaa taactctctg ggaaagtgca
     9541 gtgcaaaaag cagaggaagt tttgaatgct gatttaaatt cttatatgga tgctcgtgct
     9601 gcgatcatga gtttattaaa aatgtattat gaaatggtca aggcaaatgc tcagactatg
     9661 aaggaattgc gcgcaggaat tggtaacacg attatggatt ggggtgtctt tcccattaaa
     9721 gattatattg gaaaaaaact cacgggaaat cctgaaattg atttaccaat tttgcaacat
     9781 gaagtgagtt atactgatga caataagttg aaaattgaac cactgactcg ctcagacaaa
     9841 cgtgaggaaa aggggcaact tgataaatta tttgagggta tgatccacat gtggctaaag
     9901 aataatgatt atacccctga ccgtgataat cctggtcaat ttgttaattc acacggtgaa
     9961 accttagaca aagcaacgtt tgataaatta aaaaaagacg atcagcatgg acttaatcac
    10021 tttttaacgg aatatgatcc agatttgcaa ttcagaccaa gcagacctta attaagcaag
    10081 agaaatattg ggttacacta gcctgtatat aatacttatg ggctagtgct gaaaaatctg
    10141 ttgactctgt tttaaggcat caagccggag gcaagccact atgaaagcga aataccacat
    10201 cgtctgataa gataaacgca ttttctatag ttaaaaactc cgaaatacga tcatcaacag
    10261 agccgccata gaacttggcc aaatgcaaat actcttcaaa atgcctacat tctgatttaa
    10321 ccaatgtttt ataaaacctg gccaagtcct gatcttcaat atatggaatt attgagctaa
    10381 agcgctcgca cgatcgagcc tcaatgatag cacctataat taattgatcg catagtctct
    10441 caattccatc tttatttgtg acatgcttat gcaaattgga tgcatagtct gatggctgca
    10501 agggtgaata aacaattccc ttttgcttca tgatatcaat gactttttca aagtgcagta
    10561 attcctctct tgccaatggc gccataattg ctactaattc tgctttttct ggatatttgc
    10621 ttatgaaatt aattgctgtt ccagctgctt tacgttcaca atgagcatga tcgagcaata
    10681 aaagaggtat attggcagct gcatggttta accaggcttc cggagtttta gtttttaaaa
    10741 aatcatttaa cgtttgtaaa tcactatcta atcgttttaa catgataaaa tacactataa
    10801 tttcaagtaa atagccctta tatcacatac tttaaagaat ttaaattgga taaaaaatgg
    10861 acgagatcaa aaaagatgat gaattgagtc aatggctttc tacctatgga acaatcaccg
    10921 ctgaacgtat attaggtaga tacaacatat cactgccgca agatgaaata ctagaagcga
    10981 ttaacattcc atctagtttt tatagacatt tgttacagat tcccttaaaa aacgtactta
    11041 atggaattgt aattcaacag gcaagtgatt accatgtcta tgcacagaaa ctgttaattg
    11101 attatttgtt atctggtgaa agcagcaaag agcctgactc acagggggcc ggaactcgcg
    11161 aatcacttga agatgaaaga cagagattag ttcagttagg ggatgagttt cataaattgg
    11221 agttagagca ggataatctt attgcatcaa gccaagccag cttaatgaaa ataagcattg
    11281 actggaatac aaaactggaa acaactcttt ctaaattaaa tagcttatac aaaaatacaa
    11341 attctaaaat taaaaaaaat gcaattagaa aagcgttaat caaggccttt attcattgtg
    11401 atctggtcaa ggatcaatca caaaaaaaca agcaccagct aattgataaa ttgaatcaaa
    11461 cattagctgt ttctgtaggt gctgaattaa aagagtctat cctgaccaat ctgggtgaac
    11521 ttttccaaat cctaggcgct ttgaatacaa agcttgatga atttactgat cggactaatc
    11581 atctaagcca aaaggctaaa tcattcagaa cccaattcta tgaggtaata cttcgcatca
    11641 ttgagttaat taaactatta cccgaatata aaattgaccc ggaacaagat gcgattaatc
    11701 gagaaccatt atattttgat agaacgattg gagaacgcta acgcaaaccc ctattttctc
    11761 taaaatcata ctataactat tgactgaatg gttgaatgca tgaattttta ttagccagac
    11821 cactatgaat tcatgtataa taatgcactt taaattaatc tcattggagt gcatgatggc
    11881 aaaagaatta acattatcta ttattaaacc tgatgctgta gcaaaatctg tgataggtga
    11941 aatatatacc cgctttgaaa aagcaggctt ggatattgtt gccgctaaaa tgacacaact
    12001 ttcacgcgaa caagccgaga gcttttatga tattcatcgc gcacgaccat tctttaagga
    12061 tctggtggat tttatgattt ctggtcctgt aatgatccaa gtattaaagg gtgaaaatgc
    12121 tgtcgctaaa aacagagaaa ttatgggcgc gacaaatccc aaagaagcag cacctggaac
    12181 tatacgtgct gattttgctg atagtattga tgcaaatgct gtgcatggtt ctgacagcct
    12241 tgaaaatgct gctcgtgaaa tagcattttt ctttgaacca catgagctgt gcaatagata
    12301 aaagcaggct tgggagcggt aatggaccaa caaaaagtga atctattaaa ttataattac
    12361 tcgcagctac gagaattatt gatagcatgg gatgaaaaac ctttcagagc tcaacagcta
    12421 tttcaatgga ttcatcaagt tggcatacgt gactttgctc agatgactaa tttagggaaa
    12481 gttttaagaa ataaactttc ccaattagct tgtatagatt tacctgaaat cgttgcatgt
    12541 caaaaatcag ctgatggcac acataagtgg ctattaaagc tggaatgtgg gaattgtatt
    12601 gaaacagtat tcattccgga agcaaatcga ggaacacttt gtgtttcatc acaagtaggt
    12661 tgtgctttaa attgttcttt ttgctccaca gcaaagcaag gatttaacag aaatttatca
    12721 acagctgaaa taattggcca agtatggctg gcagcacgcg aactatcaga taataatggc
    12781 actcacgata aaaaaataac taacgttgtg atgatgggaa tgggtgaacc gttacttaat
    12841 ttcgataatg ttgtttctgc tatgaacatc atgatggatg atttagccta tggactatcc
    12901 aaaagacggg ttacattaag tacttcgggt gttttaccgg aaatggaacg actcagagaa
    12961 gtcagtcctg tcgcgttggc agtatcctta catgcaccaa cagatgaact acgtaacgag
    13021 ctggtaccaa ttaataaaaa a
//
LOCUS       DASLOE010000082        12758 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.082, whole genome shotgun sequence.
ACCESSION   DASLOE010000082 DASLOE010000000
VERSION     DASLOE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12758
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.082"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..988
                     /locus_tag="U6309_001785"
     CDS             <1..988
                     /locus_tag="U6309_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5505225.1"
                     /translation="QLVNNSVVKTEMLEGEKGVFNAFKNYKEYRSFMPKEELKTTEFG
                     VLYEANEAFLEYFYNRSESTLINKDSPFEKYFVDSSSDEEAIKNYWDAEGYILYCQHL
                     AKPQVKLSYIEIMTMMKVINQPITIYDRSTSSVVAEYVNPKVNLPDFEVAIDALQGHY
                     FLLKTEETEKELEEYERSYAQYKRDRSEILAHSDKPVSSLLVRATCPKGHLDEDPFIA
                     LIESLSEINSLSQIDTNLKNENTDTPNCNFLLKVGASVVGTSSALITLMGIAILSGQY
                     DPGNSLSEEALATTVVSSIVGLGYGLYSFFSSSPKVSELDSTNGLSQKLKMN"
     gene            1183..1602
                     /locus_tag="U6309_001786"
     CDS             1183..1602
                     /locus_tag="U6309_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEN5505226.1"
                     /translation="MSAKKNSIAHDRLLTEVELELMNIIWSLDKVTIKEVVSHLPKER
                     PLAYTTVATVLKVLEQKGFLECQKNSYAHVFMPIVTKSDYENTCIDHMVANVFDGEPR
                     ALVQRLLLARKLQHDDIQAIEEALKQLNAGEKQPEVM"
     gene            1602..3512
                     /locus_tag="U6309_001787"
     CDS             1602..3512
                     /locus_tag="U6309_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HEN5505227.1"
                     /translation="MLLIEIVLTIHCLLLVSSLFIGRAWLANKPSFKLKLLRFLLASC
                     VISPIIVHCFNFDQKPERQNYVSFDVLQQYVKQPIFKTESSKFSHESSSAFTSMNVNY
                     CQLLSVMFFLIVLFRSYHLLSGLGRLKLMLAEAIPYRTSGKLVIKVSHHCHVPFSILL
                     FNKAYIVLPVSIFSSSMNVRIAIAHEGQHHRNGDCSWAYFIEIIRIIFFGNPGVTQWF
                     RVLADLQELSCDEVLVGHQKISAHDYGHCLLQVVEAVSQCSLASNREITCTVGMALNK
                     ENEDCKFIIRRISMLTAYSPNASRPLLLGIVFAGFSILSPICVAYSAAGSLTSSRAKE
                     VDISHLDPRMQQIAEKQITTAVKQYHAKSGVIAIADPQTGNIIAFAESSKNKGLESWK
                     SRIFSPGSTIKPFIAAAAINSGNSSETKNYDCHSPYYIAGKTFTNYNSNVESASLAEA
                     IAKSINVCLIRVSQEAGVSVIRKKLTEFGFDTSTWWQADRSDDLQLAMAALGENIPVT
                     IESLIKSYAILANKGHSLDRGNSAIISETTTYSINHMLENAVTNGTGKLAVIPGVSVA
                     GKTGTVIENNDKYLALFAGYVPADNPRYVVLVVIEEGYFSKNGKTLVSGGELAAPVFR
                     NVAMDALSSANR"
     gene            3562..4362
                     /gene="blaOXA"
                     /locus_tag="U6309_001788"
     CDS             3562..4362
                     /gene="blaOXA"
                     /locus_tag="U6309_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HEN5505228.1"
                     /translation="MKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQR
                     YSPASTFKIALSLMGFDSGILKDELHPELPYKKEYELYLNVWKYPQNPHTWIRDSCVW
                     YSQALTRQLGMKRFKGYVDAFHYGNQDVSGDKGQNNGLTHAWLSSSLSISPTEQIQFL
                     QKIIYKKLPVSQKAYTMTKNIMYIQELPGGWKLYGKTGTGRQLTKDKSQKLPLQHGWF
                     VGWIEKDERVITFAKHIADSKENTTFASFRAKNDTLIQLFNLINELEK"
     gene            complement(4422..5297)
                     /locus_tag="U6309_001789"
     CDS             complement(4422..5297)
                     /locus_tag="U6309_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEN5505229.1"
                     /translation="MDKSPKLMHLLVYAVHLVLVSTCCFGEPNSPEKLLKITIANKTY
                     TLSELKKQFKTIRVIISHNPAYNYQKKTYDAFDINQILEQLMGADLQKNNADQVLLAQ
                     TLDKYVSQTPLLHFTTKGHAYLAYQETPGTISDNNITKDGRWSYINQHGKKENPGPFY
                     IVWDNTSTYPVSWPYQVISMQIVNKKELTFSHFLNPLNESASVQNGHHIFNNICSTCH
                     SLFYKGALGRAPDLGMVTSYLTPSDISKLVKNGRGYMPPIGKNLSTEEINDLIKFLIW
                     VNKQASKLKCEINSQ"
     gene            5661..7121
                     /locus_tag="U6309_001790"
     CDS             5661..7121
                     /locus_tag="U6309_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN5505230.1"
                     /translation="MNHKPIQFKDLSLIYPHKTCFQDFSGEIHFADRIALIGRNGSGK
                     STLLKMLCGLFLAPAGEIKIPHDVRFGHLPQVLEDCPDLSGGQRLNRVLSKILSDEPN
                     VLLLDEPTNHLDRRNRRSLMRMLEHYPGTLIIASHDTELINTVTDTLWHIDLGQVTVF
                     RGIYFDYQQILEDKKASIEQELSRIARQKKESHLALMKEQERNKRARVQGEKKIAQRK
                     WPTIRSHTKLGSAITTGNKRLSHINYKKQQLLEELSSLNFQEVIKPKFWLKGLGHHKP
                     LIRIQDASAAYEAVVILEDIHFQLSGCERVALHGNNASGKSTFVKAILGESQIKRTGE
                     WAVPDCNTIGYIDQHYQHLNFNETVLDLIRSSMPHASHTEIRAHLNDFLFRKNEEVEI
                     KVKNLSGGEKARLSMALIAANPPKLIILDEITNNVDLETRTHIIQVLREFQGAMLVIS
                     HDYDFLESIHIETRYQIHQGKIHHFNEKQLEVVHHD"
     gene            7114..8895
                     /locus_tag="U6309_001791"
     CDS             7114..8895
                     /locus_tag="U6309_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN5505231.1"
                     /translation="MTNRLPNHLGSFMWHFLKPYQGIVILFILFALLAGFWGPFNSLL
                     IKSFINTLAEKTSQDLSSLYWIAGLLVLNFIVFDNITWRTLGYLNYKYEAVIKNQIIS
                     QTFEYVLGGSTQFFQDNLSGRIADQITTLADNLEVILHRVSVDFLRGASLLVVSFITA
                     YFVNVLFFYILFLWFVAFASFSIWMSARLVQLSDDHASSESQLSGQLVDSLANQSNIR
                     IFSRKIYEVKRMNTFFRLVQQAFQRKELFIVLLCCAQGGMIAVMMGLASITLIYLYGK
                     GLVSIGDFALILGLSMELGHMMWYTMYQVDQFNQALGKARQSLNALVIPHDIKDKSNA
                     SQLVVTQGRIEFSKVKFHYQGGYSLFQNKSVTIESGQKVGLVGYSGSGKSTFVNLILR
                     LYEVVDGQILIDGQNLAEVTQESLRQAIAMIPQDPTLFHRSLMDNIRYGRTDASDEEV
                     ISASKKAHAHEFISLLPEGYKTLVGEHGVKLSGGQRQRIAIARAILKNAPILMLDEAT
                     SQLDSITESNIQESLWDLMQGKTTLVVAHRLSTLLHMDRILVFDKGHIIEDGTHTELL
                     NRGGLYKTLWDAQVGGFLPDQQNEKGE"
     gene            8899..9855
                     /locus_tag="U6309_001792"
     CDS             8899..9855
                     /locus_tag="U6309_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505232.1"
                     /translation="MSVKQEQCLIKVVSYDSNWPVQFEQEAERIKKVLGSNCIEIHHI
                     GSTSVPDLVAKPVIDMIPVVLDISKVDGANAAMRALGYEAKGEYGIPFRRYFQKGDNR
                     RTHHAHIFELGNSEIDRHLKFRDWMRMHPKDREAYARLKQELARQYSDDITAYCLGKE
                     DFIATIDKKAGFNGLRMVKALTTREWDKVRHFRQFYFFGKAGLSDPYTWTFEHNAHVH
                     FVLFQGSEITGYAHLQLWSHNRAALRIIVIDELKRNHQYGTRFLALCEKWLKSQGYQS
                     LHVESSPDALKFYRNNGYIDMPFDDPDGYEGDPRDTAVGKIL"
     gene            9852..10379
                     /locus_tag="U6309_001793"
     CDS             9852..10379
                     /locus_tag="U6309_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505233.1"
                     /translation="MIQIEIVIKTLELSDIPLLVDAFQKANWEKPASLFEAYYQEQRK
                     SLRIVWLAYSQDQIAGYVTLKWISQYQPFAHQKIPEIMDLNVLPSFRKQGVGTALLQA
                     AEEKAAGESDVVGIGVGLYGGHDGGYGQAQRLYVNRGYIPDGLGVTYGYKSTVPGQSY
                     PLDDDFILWFTKKLR"
     gene            10445..11197
                     /locus_tag="U6309_001794"
     CDS             10445..11197
                     /locus_tag="U6309_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN5505234.1"
                     /translation="MAYTVKQLAKISNVSVRTLHWYDEIGLLKPAYQGANGYRYYEEK
                     QCLKLQQILFFRELGFPLNDIQKLLMQDDFDHIKALRAHKQVLESDINRKNQLIVTID
                     KTIQHLRGKHKMSNEELYYGFDSLRQKQYEKELVKEGVVSQDWMNNYKKKTSQWTQAD
                     KDRFIQEGKDINQALISAINNQLKPASPEVQAIIHRHHAWVGWNPSKEGYIGLSQRYL
                     TPEFRKFYEWQHPDLLEFIIEGMLFYANKNLD"
     gene            11449..12396
                     /locus_tag="U6309_001795"
     CDS             11449..12396
                     /locus_tag="U6309_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN5505235.1"
                     /translation="MTQWFVKDLSQLTGVSVQTLHHYDRIGLLKPSLRLANGYRVYSE
                     KDLLKLQQIIALKFFGFELSQIKTLLAEEGNALNHFNSQARVLEQKASTLLEGAKTLR
                     RIIDSVDKNQSIPWETIIQLIEVYKMTEHLEHGWVKEIFTPDELKQYAEFEKEMKANA
                     TPEQKAAMENNWSQLVDEVKNNLKQDPDSKIVIYLGKKIMDWVNGVYGKKYAHLRTKK
                     FEQGFGEGKGLDEVGLTPEIVSWMDKAMDAYWRDRIYGILDQVGKAPSASILDLWNEM
                     LVDMYGEETSRKNAIYEIALNDDQVSPEAKAWLRNILDS"
BASE COUNT         4023 a         2216 c         2588 g         3931 t
ORIGIN      
        1 tcaacttgtt aataattcag tagttaaaac agaaatgctg gaaggtgaga aaggagtatt
       61 caatgctttt aaaaattata aagagtatcg ctcatttatg ccgaaggaag aattaaaaac
      121 aacggaattt ggtgtgttat atgaggcgaa cgaggctttt ttagagtatt tttataatcg
      181 ctctgaaagt actttaataa ataaggattc accttttgaa aaatattttg ttgactcgtc
      241 atcggatgaa gaagcaatta aaaattattg ggacgcagaa gggtatatat tatattgtca
      301 acatttggcg aaacctcagg ttaaattatc ttatattgaa attatgacca tgatgaaggt
      361 cattaatcag ccaattacaa tatatgatcg ttctacttcc tcagttgtag ccgaatatgt
      421 caacccaaag gtcaatttac ctgattttga agtggctatt gatgctcttc aaggacatta
      481 ttttctttta aaaacagagg aaacggaaaa agagttagaa gaatatgaaa gaagttatgc
      541 tcaatataaa agagatagaa gtgaaatcct tgcacactcc gataaacctg tttcttcctt
      601 attggttcga gctacttgcc ccaaaggtca tttggatgaa gatcctttta ttgcattaat
      661 tgaaagcctt tccgaaataa acagccttag ccaaattgat actaatttga aaaacgagaa
      721 tacagatacc cccaattgca acttcctttt aaaggtaggt gcttctgtag ttggtacctc
      781 ttcagcacta ataactttaa tggggatagc catattatct ggccaatatg atcccggaaa
      841 cagtctgtct gaagaggcat tagctactac tgttgtatcc agtattgtcg gtttaggtta
      901 tgggctttat agcttcttta gttctagccc taaggtatca gagcttgatt ctaccaatgg
      961 attatcgcaa aaacttaaaa tgaactaacc tcctgaatta gcaaatattg tctgtaagcc
     1021 gatcgtgttg atattagagg tatcaacacg attgcgccat tcaagtaaac cgttctcgcg
     1081 tgtagagaag tatttttccc atgactattt caactctact atttttatag tagaatccta
     1141 ctataaaaat agtagagact tatgtggtta ttggagagaa acatgtcggc aaaaaaaaat
     1201 tcgatagctc atgatcgact tcttacagaa gtagagcttg aattaatgaa tattatctgg
     1261 agccttgata aggtaactat taaggaggtt gtttcacatc tgcctaagga aaggcctttg
     1321 gcttatacaa ccgtggcaac agtgctaaaa gtgctggaac aaaagggatt tcttgagtgt
     1381 caaaaaaatt cctatgctca tgtttttatg cccatagtta caaagtcaga ttatgaaaac
     1441 acttgtattg atcatatggt tgccaatgtt tttgatggag agccccgagc acttgtccaa
     1501 agacttttat tagccagaaa gttacagcat gatgacattc aagccattga agaagcactt
     1561 aaacaattaa acgctggtga aaaacagcca gaggtgatgt aatgttatta atagaaatcg
     1621 tattgactat tcattgcctg cttcttgtca gttcactatt tatagggcgt gcctggttag
     1681 caaataaacc atcttttaaa ctgaaattac tccgtttttt attggccagt tgcgttattt
     1741 caccaataat agttcattgt tttaattttg atcaaaagcc tgaacgacaa aactatgtat
     1801 catttgatgt tttgcaacag tatgtaaagc aacccatatt taaaacagaa tcttcaaaat
     1861 ttagtcatga atcgtcttct gcctttacat ctatgaatgt taactactgt caactcttaa
     1921 gcgtgatgtt ttttttaatc gtcttgtttc gtagttatca cctgttatca ggtttaggtc
     1981 gcttgaaatt gatgttggct gaagcaatcc catatcgaac ctccggaaaa ctagttatta
     2041 aggtttctca ccactgccat gttcctttct caatattgct atttaataaa gcctacatcg
     2101 tattgccagt atccatattt tcttcttcca tgaatgtgag gattgctatt gctcatgaag
     2161 ggcaacacca tcgtaatgga gattgttcat gggcctattt tatcgaaatc atacgcatta
     2221 tcttcttcgg aaacccgggg gtaactcaat ggtttcgtgt tttagcagat ttacaagaat
     2281 tgtcatgtga tgaagtattg gttggacatc aaaaaatttc ggcacatgac tatggtcact
     2341 gtctattgca agtggtagag gcagtatccc aatgctctct ggcatctaac cgggaaatta
     2401 catgcacggt gggtatggct ttgaacaaag aaaacgaaga ctgcaaattt ataataagga
     2461 gaatttcaat gctaactgct tattcaccca atgcatccag gcctttgcta ttagggatag
     2521 tctttgcagg attttctatc ctgtcaccaa tctgtgttgc ctattcagca gcaggatctt
     2581 tgacgagttc cagggcaaaa gaagttgata tttctcattt agatcccaga atgcagcaga
     2641 ttgcagagaa acaaattacg acagccgtca aacaatatca tgcaaaatct ggagttattg
     2701 caatagctga tccccaaaca ggcaatatca ttgcatttgc tgaatcaagt aaaaataagg
     2761 ggttagagag ttggaaatca cgcatttttt cacccggatc tactataaaa ccttttattg
     2821 cagcagctgc aatcaactct gggaatagct ctgaaaccaa aaattatgat tgtcattcac
     2881 cttattacat agcaggcaaa acatttacaa attataattc caatgttgaa tcagcttcct
     2941 tagcagaagc catcgcaaaa tctatcaatg tatgccttat cagagtttct caagaagcag
     3001 gagtgtcggt aatccgtaaa aagctcactg aatttggttt tgacacaagc acttggtggc
     3061 aagcagatcg aagtgatgat ttacaactgg caatggctgc acttggagag aatatccctg
     3121 tcacaatcga atcgctaata aaatcttatg ccattcttgc taacaaagga cactcacttg
     3181 acagaggaaa tagcgccatt atttcagaaa caaccaccta ctcaataaat catatgcttg
     3241 aaaatgctgt tacaaatggc actggaaaac tcgcagttat tcctggtgtt tctgtagctg
     3301 gtaagacagg aactgtaatt gaaaataatg ataaatatct tgctttgttt gccgggtatg
     3361 tgccagctga taatccgcgt tatgttgtat tagtcgtgat tgaagagggt tatttcagta
     3421 aaaatggaaa gacattagtt tcgggaggag aattggccgc accggtattc cgaaatgtcg
     3481 caatggatgc tctgagcagt gcaaatcgat aactagctta tgggtttgca tatccatata
     3541 ttcaagaatg ttgaggttgt tatgaagaaa ataattatat ttttgtgcac aggattatta
     3601 ttttgcgtct ctacttgggc tcaaggcacc tgttttttag caaaagaaaa tcaaactgtt
     3661 ttaaaacgtg agggcaatga ttgtgatcaa cgctattcgc cagcatcaac ttttaaaatt
     3721 gcccttagcc tcatgggctt tgattctgga atattaaagg atgagttgca tcctgaattg
     3781 ccatacaaaa aagaatatga actttatctg aatgtatgga agtaccctca aaacccgcat
     3841 acctggatca gagactcctg tgtttggtat tcacaggcat taactcgaca attgggcatg
     3901 aaacgcttta aagggtatgt cgacgcattt cactacggta atcaggatgt ttcgggagac
     3961 aaaggtcaaa ataacggttt aacccatgcc tggctttcaa gctcactttc tatctcaccc
     4021 acagagcaaa ttcaatttct acaaaaaata atctataaga aactaccggt gagtcaaaaa
     4081 gcttatacca tgactaaaaa tatcatgtac atccaggagt tgcctggtgg atggaagctt
     4141 tatggaaaaa caggtactgg tcgacagtta acaaaagata aaagtcaaaa attgccttta
     4201 caacacggat ggttcgtagg ttggattgaa aaggatgagc gtgtgattac ctttgcaaaa
     4261 catattgcag acagtaaaga gaataccact tttgccagtt ttagagcgaa aaatgataca
     4321 ttaattcaat tgtttaacct gattaatgaa ctggagaaat aatccaaaag atggccggtt
     4381 cagtgttttc tgatccggct atctaattcc ctgaattata atcactggct gttaatttcg
     4441 cattttagtt tggatgcttg tttattaacc cagattaaga atttaatgag atcattaatt
     4501 tcttccgtag agaggttttt gcctatcggt ggcatgtatc ctcgcccatt ctttacaagt
     4561 ttactgatat ctgaaggcgt taaataactc gtcaccatac ctaagtctgg tgctcgtcct
     4621 agagccccct tgtaaaaaag agagtggcat gtggagcata tattattaaa aatatgatga
     4681 ccattttgca cagaagcaga ttcatttaat ggatttaaaa aatgagaaaa agtcagctct
     4741 ttcttattta ctatttgcat cgaaataacc tggtatggcc aactcacagg gtaggtactt
     4801 gtattatccc atacaatata gaaaggacca ggattttctt ttttaccgtg ttgattaatg
     4861 taactccaac gaccgtcttt ggtgatatta ttgtcagaga ttgttccggg cgtctcttga
     4921 taagcaagat aggcgtgccc ctttgtcgta aaatgcaaca agggagtttg agacacatat
     4981 ttatctagtg tttgagctaa taatacctgg tcagcattgt ttttttgtaa atctgctccc
     5041 atcaattgtt ctaaaatctg atttatgtca aacgcatcgt atgttttttt ctgataatta
     5101 tatgcaggat tatgagagat aatcacacga atggttttaa attgtttttt tagctccgac
     5161 agggtatatg ttttattggc aatagtaatt ttaagtagtt tttctggtga attgggttct
     5221 ccaaaacaac atgttgagac taataccagg tgaaccgcat aaaccagaag atgcattaat
     5281 tttgggctct tgtccataag tccttcttgt ttgttgaaaa caaatcagat ttttcccatc
     5341 ctgttactag ctattatggt ttataacatg gacacttcca agtgatattt tccgacatgc
     5401 agactcattt atctcaaacg agaggcgcta ataagctacc aggagaactc gataagccag
     5461 gaaaaataat tttgaatata atattgacat cttctactat tttaatttat atttcatgta
     5521 atttttttac cagttaaaag gtaggtttag gccgtcatga aaattacatc ttaagtacgc
     5581 ccaactatag ggcaaagtga acaaagtcct ttgtgacttt tagtttttac tgtcctggca
     5641 gacatgctta agagtaaatc atgaaccata aaccgattca atttaaagac ctcagtctta
     5701 tctatccgca taaaacctgt tttcaagact ttagtggtga aatccatttt gctgatcgca
     5761 tcgcactgat tggacgtaat ggctcaggaa aatcaacact actcaagatg ctttgtggtc
     5821 tttttttagc ccctgcgggc gaaattaaaa taccccatga tgttcgcttt ggtcatttac
     5881 cccaagtgct tgaggattgt cctgatttaa gtggcggtca gcgattaaac cgagtattaa
     5941 gcaaaatact gtcggatgag cctaatgttt tgttgttaga tgagcccaca aatcatctgg
     6001 acaggcgtaa tcgtcgttct ttaatgcgca tgcttgaaca ttatcctggg acattgatca
     6061 tagcctctca tgatacagaa ctcatcaata cagttacaga tactctatgg catattgatt
     6121 taggtcaagt gaccgtattc agaggaattt attttgatta tcaacaaatt cttgaagaca
     6181 agaaagcatc gattgaacag gagttatcaa ggattgcaag acagaaaaaa gaatcacatc
     6241 ttgctttgat gaaagagcag gagcgtaata aacgtgcgcg tgtccagggt gaaaagaaga
     6301 tagcccaacg caaatggcca acgattcgat ctcatacaaa gctcggttca gcaattacaa
     6361 caggcaataa acgattaagt cacattaact ataaaaaaca acaattactc gaggagcttt
     6421 cctctctaaa ctttcaggag gtaattaagc ccaaattttg gttaaaggga cttgggcacc
     6481 ataaaccctt aatcaggatt caagacgctt ctgctgccta tgaggctgtt gtgattcttg
     6541 aagatattca ttttcaactt agcggttgcg aacgagtggc attacatggc aacaatgctt
     6601 caggtaagtc gacctttgtc aaagcgatct tgggggaaag tcaaattaag agaacagggg
     6661 aatgggctgt gcctgattgc aatacaatag gctatatcga tcaacattat caacatttga
     6721 acttcaatga aacagtcctg gatttaatac gctccagtat gccccatgca tctcatacag
     6781 aaattcgtgc tcatttaaat gattttttat ttcgcaaaaa tgaagaagta gaaattaagg
     6841 tcaaaaatct ttctggtggt gaaaaggcaa ggttatcgat ggcattgatt gctgcaaatc
     6901 cacctaaact aattatttta gatgagatca cgaacaatgt agatttggaa acacgcactc
     6961 atatcattca ggtgctgcgt gaatttcaag gtgcaatgct ggttatttct catgactatg
     7021 actttttaga atccattcac attgaaacca ggtatcagat tcatcaagga aagatacatc
     7081 atttcaatga gaaacaatta gaggtagtgc atcatgacta atcgtttgcc taatcactta
     7141 ggctctttta tgtggcattt tttaaaacca tatcaaggca tagtgatatt atttatcctg
     7201 tttgccctat tggccgggtt ttgggggcca tttaatagct tattgattaa gtctttcatt
     7261 aatactttgg ctgaaaaaac aagtcaggac ctatcttctt tatattggat tgctggatta
     7321 ttggtattga actttattgt ttttgacaat ataacctggc gaacacttgg gtacttgaac
     7381 tataaatatg aagcggtcat taaaaaccaa atcattagcc aaacatttga gtatgtgtta
     7441 ggaggaagca cgcaattttt tcaggataat ctatccgggc gaattgccga tcaaatcacg
     7501 acccttgctg ataatcttga ggttattttg catcgtgtgt ctgtagattt tcttcgtggc
     7561 gcttcattat tagtggtttc atttattaca gcctattttg ttaatgtctt gttcttttat
     7621 attttgtttc tctggttcgt cgcctttgcc tcatttagta tttggatgtc agcgcgatta
     7681 gtccagttgt ctgatgatca cgcaagttct gaatcacagc tttccgggca gttggtagat
     7741 agcctggcga atcaatccaa cattcgtatc ttttcaagaa aaatctacga agtgaaacgt
     7801 atgaacactt tctttcgctt ggtacaacag gcttttcaaa gaaaagagct ttttattgtt
     7861 ttgttatgtt gtgcacaagg aggaatgatt gcagtcatga tgggtttggc atcgattact
     7921 ttaatctatt tgtatggtaa aggtcttgtg agcattggtg attttgcctt gattctcgga
     7981 ctatccatgg aattgggtca tatgatgtgg tacaccatgt accaagtgga tcaatttaac
     8041 caagccttag gcaaagcaag acaaagtttg aatgcattgg tgatacctca tgacattaaa
     8101 gataaaagta acgcatctca attagtagtt acccaaggac gaattgaatt ttccaaggta
     8161 aaatttcatt accaaggcgg ttattctctg ttccaaaata agtctgtaac gattgaatca
     8221 ggtcaaaaag tgggtttagt aggctattca ggcagtggta aatcgacttt tgtgaatctg
     8281 attttacgac tttatgaggt tgtggatggc cagattctca ttgatggtca aaaccttgcc
     8341 gaagtcacgc aagaaagttt aaggcaagct attgccatga ttccgcaaga cccaactctt
     8401 tttcatcgaa gcctaatgga caatattcgt tatggcagga cagatgcttc tgatgaggag
     8461 gtcatttcag cttccaagaa agcccatgca catgaattca taagtctttt gccagaaggc
     8521 tataaaaccc tggtagggga gcatggggtt aaactttcag gtggtcagcg ccagcgcatt
     8581 gcgattgccc gagccatctt aaaaaatgct ccgattttaa tgctggatga ggctacgtct
     8641 cagcttgatt ccattacaga atccaatatt caagagagcc tctgggattt aatgcaggga
     8701 aaaacaaccc tggtagttgc tcatcgcttg tctacccttc tgcatatgga ccgaatttta
     8761 gtattcgata aaggccatat tatcgaagac ggtacgcaca ctgaactatt gaatcgtggc
     8821 ggtttgtaca aaaccttgtg ggatgcccaa gttggtggtt ttttgccaga tcaacagaat
     8881 gaaaaaggag aataagcaat gtcagtcaag caagagcagt gtctcattaa agtggtttct
     8941 tatgattcga attggcctgt gcaatttgaa caggaagcag agcgtattaa aaaagtgtta
     9001 ggcagtaatt gcattgaaat tcatcatatt ggttccactt cagtacctga tttggttgca
     9061 aaaccagtca ttgatatgat tccagttgtt ttagatatta gcaaagtaga tggagccaat
     9121 gctgccatgc gagcacttgg ctatgaagca aaaggagaat acggtattcc ctttcgccgt
     9181 tatttccaaa agggggataa tcgacgaacg catcatgctc atatttttga acttggaaac
     9241 tctgaaatag atcggcattt aaagtttagg gattggatga gaatgcaccc gaaagataga
     9301 gaagcttatg cacgtttaaa acaagagttg gcgcgccaat attctgatga cattaccgcc
     9361 tattgcctgg gtaaagaaga ttttattgca acaattgata aaaaagccgg gtttaatgga
     9421 ttaagaatgg ttaaggcact aacaacacgt gaatgggata aggtgcgtca tttcagacaa
     9481 ttttattttt ttggcaaagc agggctttct gatccttata cctggacttt tgagcataat
     9541 gcgcatgttc attttgtttt atttcaagga agtgaaatca caggttatgc tcatctgcaa
     9601 ttatggtctc ataacagagc agcactacgg attatcgtta tcgatgagct taaaagaaac
     9661 catcaatatg gtacccgttt tttggcatta tgtgaaaaat ggctcaaaag tcagggctat
     9721 caaagtttgc atgtcgagtc gtcaccagat gctctgaaat tttatcgaaa taatggttat
     9781 atcgatatgc cttttgatga tcccgatggc tatgaaggtg atccacggga tactgcagta
     9841 ggaaaaatat tatgattcaa atagaaattg tcattaaaac actggagtta tccgacatcc
     9901 ctcttctggt tgatgcattt cagaaagcaa attgggaaaa acccgcttca ttgtttgaag
     9961 cctattacca agaacagcga aagtctcttc ggatagtatg gttggcttat tctcaagacc
    10021 aaattgctgg ttacgttact ctaaaatgga tatcgcaata ccagccgttc gcccatcaaa
    10081 aaattccaga aattatggat ttaaatgtat tgccctcttt tcgtaaacag ggagtgggga
    10141 ctgctttact gcaagccgct gaagaaaaag cagcaggcga aagtgatgtc gtggggattg
    10201 gcgttggttt atatggcggc catgatggag gatacggaca agctcaacgg ctttatgtca
    10261 atcggggtta tatacctgat ggtttaggtg tcacttatgg ttataagtca actgtcccag
    10321 ggcagagcta tcctttagat gatgatttca ttttatggtt tacaaaaaaa ttaagatgaa
    10381 atgcttgacc cttacgttac gtgagcactt atctttaaat ggaatcattt gagaggacag
    10441 tttaatggct tatacggtaa aacaattagc aaaaatatcg aatgtaagtg tgcgcacttt
    10501 acattggtat gatgaaatag gcttattaaa accagcctat caaggtgcta atggctatcg
    10561 ttattatgaa gaaaagcagt gcttaaaatt acagcaaatt ttattcttca gagagctggg
    10621 atttcccttg aatgatattc aaaaactgct tatgcaagat gattttgacc atatcaaagc
    10681 attgagagcg cataaacagg ttttggaaag cgatatcaat cgaaaaaatc aattgattgt
    10741 aacgattgat aaaaccattc aacatttaag aggtaagcat aagatgtcta atgaagaatt
    10801 atattatggt tttgattcac tgcgacaaaa acaatacgaa aaggaattag ttaaagaagg
    10861 ggtggtcagc caggattgga tgaataacta caagaaaaaa acaagtcaat ggactcaggc
    10921 tgataaagac cgatttattc aggagggtaa agatatcaat caagcactga tttcggctat
    10981 taataatcaa cttaagcctg cctctccaga agtacaagcc attatccata ggcaccatgc
    11041 ttgggtaggt tggaacccaa gcaaggaagg gtatattggg ttaagccaaa gatatttgac
    11101 tcctgagttt agaaagttct atgagtggca gcatcctgat ctgcttgaat tcattattga
    11161 agggatgctg ttttatgcaa acaagaacct tgattaagct gagagaaggg ctgtgtctta
    11221 taagccctaa atgatattgg attgagagca ttgtgcgtgt tagcagaaat aatactgatt
    11281 tatacatgcc ggcttatcat gttgttccaa accaggagct tttgtgagtt ttttgcttaa
    11341 taattgatct cttggtaggt tagaaaaata atgactcaag aaattttaaa aactgcttga
    11401 ccctattgtt gctataggcc ttacctttgt cttgaagggg gaaacacaat gacacaatgg
    11461 ttcgtaaaag atttaagcca attaacaggt gtatcagtgc aaacgttaca tcactatgat
    11521 cgtataggat tgctaaagcc ttcattacga cttgccaatg ggtaccgtgt ttactctgag
    11581 aaggatttat tgaaattaca acagattatt gctttgaagt ttttcggttt tgagttgtca
    11641 caaatcaaaa ccttactcgc tgaagaaggt aatgcgctca atcattttaa cagccaagct
    11701 cgggtgttag aacaaaaagc atccacttta cttgagggag ctaaaacttt gagacgcatc
    11761 attgactctg ttgacaaaaa tcaatctatc ccttgggaaa ccattattca acttatagag
    11821 gtatataaaa tgacagaaca tcttgagcat ggctgggtaa aggaaatatt cacgccggat
    11881 gaattaaaac agtacgctga gtttgaaaag gaaatgaaag ccaatgccac gcctgagcaa
    11941 aaagcggcta tggaaaacaa ttggtctcag ttagttgatg aggtaaaaaa taatctcaag
    12001 caagatccag actcaaaaat agtaatttat ttaggaaaga agattatgga ttgggtaaat
    12061 ggggtctatg gtaaaaaata tgcccattta aggaccaaaa aatttgaaca gggttttggg
    12121 gaaggaaaag gtcttgatga ggttggatta acaccggaaa tcgtatcctg gatggataaa
    12181 gcgatggatg cttactggag agaccgaatc tatgggattc ttgatcaagt aggaaaagct
    12241 ccttcagcaa gcatattaga tctttggaat gaaatgctgg ttgatatgta tggtgaggaa
    12301 acttcacgaa aaaatgccat atatgaaatc gctttaaatg acgatcaggt tagccctgag
    12361 gcaaaagcct ggcttagaaa tattttagat tcctaaaaat atcaataaca ctgttggatc
    12421 ggtattgttt taccgatcca ttcaactcga tttgatacct tcaattatta attgatttag
    12481 catcttaggc agaccaatcg tctctaaata aatttaattg ctatagacac aataaaccat
    12541 gattttcaaa cttctgttta atgacataat ttaatatttt aaggaaaagt tataaatgaa
    12601 gtatcaatta gaacttgttg cagatcttgt aatgccctta ataaacctta gtttgaatct
    12661 ggtgtggtca cgtttgaagg tgaactaaaa tggtatatac ccatagcgat aaaaaaacat
    12721 ggtttttgcc cagaataagt attatgggat ctttatga
//
LOCUS       DASLOE010000083        12743 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.083, whole genome shotgun sequence.
ACCESSION   DASLOE010000083 DASLOE010000000
VERSION     DASLOE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12743
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.083"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(48..773)
                     /locus_tag="U6309_001796"
     CDS             complement(48..773)
                     /locus_tag="U6309_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEN5505236.1"
                     /translation="MENLEHGHSQKEMRERLAQIPSQNYLRDWIYGGIDGVVTTFAIV
                     SGVVGGQLSSLVILILGFANLLADGFSMAASNYLGTKSEVDQFQRYKTIEEKHIDFIP
                     EGEKNEIKQIFQNKGLHGQTLDQVVEEITANRALWIKTMLQEEYGLPAALRFPLKSAL
                     YTFSAFLLFGIIPLIPYVLVMNNSFIWSCFFTAITFFVIGSIKSHWSTKSWLYSGFET
                     LIIGTVTASLSYGIGLFLHHLLT"
     regulatory      877..970
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1045..1989
                     /locus_tag="U6309_001797"
     CDS             1045..1989
                     /locus_tag="U6309_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN5505237.1"
                     /translation="MSSLKSRVTLLLNWYTNPYHTPILVAQQLGFYSEEDIKLAILEP
                     ADPSDVTEIVGLGTVDFGVKAMIHTVAAKAKGYPVTSIGTLLDEPPTGLIALKSSGIN
                     SFQDIVGKRVGYIGEFGKKIIDDLASLAGIDPTSYKTVRIGMNVTDAIYRDIIDTGIG
                     FINFQKVELEHLCGETVFLRIDQLAGLGCCCFCSIQFIVPETTLQQPELVKGFLRATQ
                     RGAAYTTEKPEEAYELLCQAKPQLRTPLYQKIFTRTLPFFSRTLINVDRDWDKVGRYT
                     KHLKIIDEHFDISQCYTNRFLPDIPYSDLKPIACCLEN"
     gene            1992..3062
                     /locus_tag="U6309_001798"
     CDS             1992..3062
                     /locus_tag="U6309_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN5505238.1"
                     /translation="MRAGIVGSGIMGRLLAVALHNAGWQVSLFDRSNESMSCSTAAAG
                     LLTPFSELDKSILLISTLGQESIKNCWPRIIEDLPEAVYFQQSGSLVLCHPGDQAEWI
                     HFSRRILSQLNPSNSVFQKLSHENLIALEPELTKFETAYYFPNEAHIDCQTLMLSLKN
                     YLDSVCIPHITNTPVLSLKPGEINTKEQRYHFDVVFDCRGLGAQNIFSDLRGIRGELI
                     WLHAPGVQLHRPIRLLHPRYSLYLVPRPGNLYLIGASEIEAEDFSPVSVRTTLELLTA
                     VYYLHGNFDEARIIKTVTHCRPTLANHLPRIKFADGLIAVNGLYRHGYLIAPALAEEI
                     LRGVTSNFRHLNYPELWESYCD"
     gene            3055..3258
                     /gene="thiS"
                     /locus_tag="U6309_001799"
     CDS             3055..3258
                     /gene="thiS"
                     /locus_tag="U6309_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEN5505239.1"
                     /translation="MINVYFNDKIYKIQPSLSLSAFLKQLNYTQQHFAVAINNHHVPH
                     KAYEATILQANDRVDILFPMQGG"
     gene            3261..4049
                     /locus_tag="U6309_001800"
     CDS             3261..4049
                     /locus_tag="U6309_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEN5505240.1"
                     /translation="MWNLAGKELSSRLLLGTACYPSLEHMQQAILNSGTEVITISIKR
                     QTSAGLDGKSFWQAVKKLDCHLLPNTAGCRNAEAAINTAEIARELFDTHWIKLEVIGD
                     DYNLQPEPFELIKATRILIDRGFEVFPYCTDDLVLCQKLVDAGCKILMPWGAPIGSGK
                     GLINPYALETLRYRFPDITLIIDAGIGKPSHAVQAMEFGFDGVLLNTAVALANHPALM
                     ATAFRHAVIAGHQAFTGGMMSERNVAHPSTPLIDTPFWHQVNNL"
     gene            4046..5512
                     /gene="thiD"
                     /locus_tag="U6309_001801"
     CDS             4046..5512
                     /gene="thiD"
                     /locus_tag="U6309_001801"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEN5505241.1"
                     /translation="MKKPIVWTIAGIDSSGLAGVHADMETFSRLNVRACSVITAVTAQ
                     NAHSITAVEEISRDQVAAQCRALELNLKPDAIKIGMLCSTPICEEIACFLKGYEGFVV
                     LDPIITSSSGTNLFFSDLQQHKKNLIQLFRYVTIITPNRIEAEAILNRSISSYQDIIN
                     AASDLLSLGAKQVLLKGGHLKDNLFSQDYWTDGKESFWIANHRFPETNYRGTGCVLSS
                     ALTACLALGYSIKDAIVIAKMYVNRGIRQSIEIDKDASQLYHDGWPEDEADLPYLSPT
                     PFIKPVPSFKKYCMGFYPIVDSSHWLEMLLPLGVKCIQLRIKEASQERLEEEIKRSVH
                     LANQYNASLFINDYWELAIHYGAAGVHLGQEDLEKADIDRINRSGLFLGISTHCYYEV
                     ARAHALNPSYVACGPIYETTSKIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSD
                     VLKTKIDGVSVISAITKASAPLVAAKQFLTQMNESHNE"
     gene            5505..6632
                     /locus_tag="U6309_001802"
     CDS             5505..6632
                     /locus_tag="U6309_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEN5505242.1"
                     /translation="MNKSNLAMNELTRYSQQIKMEEIGLDGQEKLKNSRVLCIGLGGL
                     GSPLLLYLAAAGVGVLGIVDDDIIELSNLHRQILYNHTHINKKKAVTAKKQLLAINPL
                     IQVESYSSRLTEENAAELITQYDIIADGTDNFYTRYLIHSICFELEKPYVYASASGFQ
                     GYCSIFHGKQDPCLHCIFPVSVNDINMPNCEGVGVIGTLPGMLGIIQATEIIKWILQI
                     GNPLKKRLLAINLLTMTFKNIIISKNPDCEFCIQSQSIRQLINPVNCSTQIEPCHHRI
                     TLDEFYQLFTNNKNIHLIDVRSTKEHQIQNIGGIVLPLDELPQRLHELNPNRLIILYC
                     QSGKRSKMALDILLKAGFASVKYIENGLKGVTANYNKDMPQ"
     gene            complement(6661..7494)
                     /locus_tag="U6309_001803"
     CDS             complement(6661..7494)
                     /locus_tag="U6309_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEN5505243.1"
                     /translation="MIRSILVSFFLFIVNAGYAWNAIGHQLVAQIAYDNLTPQSRRMC
                     DLYSHSKSKTSSNVNFVKSASWLDSIRAHDVHWFDALHYIDIPFSMDETELPALPDIN
                     ALWGINQAIAVLSSNKASIADKKLSLRILVHLVGDIHQPLHTVTKISKKLPKGDLGGN
                     LFQLAKNPIGNNLHQYWDNGGGILIGQDKFFQIKNKARQLEKKWSCQSASKDKNPQQW
                     INASHQLALTKVYKVSAHQVPGKQYQLNTQNITEKQILLAGCRLAYLLNNIAEGKNKL
                     I"
     gene            complement(7497..8756)
                     /gene="tolB"
                     /locus_tag="U6309_001804"
     CDS             complement(7497..8756)
                     /gene="tolB"
                     /locus_tag="U6309_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEN5505244.1"
                     /translation="MFNRIISLFLLLFTGQVIALDLELTQGINSALPIAINSFGSDAA
                     AQEIGNVIENDLTISGQFKIISGPQGANSQSSVSTLRQLGADSVVTGRVNQVGNRIEV
                     SFTLADAVANGNILLTKTFQINANQVRALAHHISDEVYQKLTGERGIFSTRIAYISVQ
                     RNGGRSRYSLEVADADGHNPQSLLVSSEPIMSPSWSPNGKSISYVSFEKKKAEIFTVS
                     VETGQRRLITSFPGINGAPAWSPDGRHLAVVLSKSGTPKIYDVDLSSGSMRQLTFGNS
                     IDTEPRYSPDGRSLLFTSGRGGSPQVYRLSLADGQISRVTFEGNYNARASYTPDMKHI
                     VMLHREDRQFNIGVQNTGGGPISNLTFSGLDESPSVSPNSRLVLYATRYQDRGVLGIV
                     SIDGRIRMRLPAREGDVQEPAWSPYLS"
     gene            complement(8762..9760)
                     /gene="tolA"
                     /locus_tag="U6309_001805"
     CDS             complement(8762..9760)
                     /gene="tolA"
                     /locus_tag="U6309_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEN5505245.1"
                     /translation="MISDLSYRKAFLFAVTLHLFLLVLLFTDNSSKRPVLTMESKNSP
                     GMMQPTASQQEVVKAVSVDNKEIMKTVNRLKQEREQQKKAELNRQKELLRQAEAAKQQ
                     RIKEQQEIARLKEEANKIAIARKKQVEEEKKRLKELAEQKALEAKRIEELKKQNEKLA
                     KQRELEAKKLAELNKKKQEEKEKAEKLKAEQAKAEKLKAEKARAEQEKAEMERKRQAE
                     AAAAQQAAQNAERQARIAGEVDKYKALIVNAIGRNWILPENVDSSLSSQFRIRLAPDG
                     TVLEVSLIRSSGDPLLDRSAQTAIYKASPLPVPQDVETFNLFRDISLTVRPEQVRG"
     gene            complement(9757..10212)
                     /gene="tolR"
                     /locus_tag="U6309_001806"
     CDS             complement(9757..10212)
                     /gene="tolR"
                     /locus_tag="U6309_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEN5505246.1"
                     /translation="MNRSKTNRERPISEINVVPYIDVMLVLLVIFMITAPMLTQGVTV
                     DLPKAASQTLQAADREPIIVSVNQLGSYFLNISSTPAEPIEAQALLVRVAAELELAKQ
                     SGQKLNVLVKGDQGVAYGKVVQAMALLKQAGAEQVGLLTDSQTGDEGQA"
     gene            complement(10221..10898)
                     /gene="tolQ"
                     /locus_tag="U6309_001807"
     CDS             complement(10221..10898)
                     /gene="tolQ"
                     /locus_tag="U6309_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEN5505247.1"
                     /translation="MVANQANVLMYFMQAGLVVKSVMLILLIASIISWTLIFQRAWFF
                     NRRKQMTETFTRRFWDCGDLSKLYADVDSNSDERQGVAAIFHAGFKEFVRCRKQGKVS
                     IEPIQRVMQISHAKEAEKLEQHLPFFASVGSISPYVGLFGTVWGIMTSFQALGHAQQA
                     TIAMVAPGISEALVATALGLFTAIPAVIAYNRYTTRANTLLNKYDLFQEELISLIEQQ
                     TSGSPRG"
     gene            complement(10923..11318)
                     /gene="ybgC"
                     /locus_tag="U6309_001808"
     CDS             complement(10923..11318)
                     /gene="ybgC"
                     /locus_tag="U6309_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEN5505248.1"
                     /translation="MNLISNHQHNIRVYAEDVDYMGIVYHANYLCYLERARTELLRQN
                     NLILSEFEKSDVLFAIHELTINYVFPARLDDQLTIKTEIKEFRACSFLFNQEISNQHK
                     QLICRASVQVVCVGSNLKPKRLPNIMRNE"
     gene            complement(11322..12332)
                     /gene="ruvB"
                     /locus_tag="U6309_001809"
     CDS             complement(11322..12332)
                     /gene="ruvB"
                     /locus_tag="U6309_001809"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEN5505249.1"
                     /translation="MLESDRLISSQSIVSEDAMDRAIRPLSLSEYVGQDSVSSQMQIF
                     INAARKRNDPLDHVLIFGPPGLGKTTLANIIAHEMGVNIRQTSGPVIERAGDIAAILT
                     NLQQNDVLFIDEIHRLSPVIEEILYPAMEDYKLDIMIGEGPAARSIKLELPPFTLIGA
                     TTRAGLLTSPLRDRFGIVQRLEYYSVDSLTKIVARSAHLLGVPTKPEGAREIALRSRG
                     TPRIANRLLRRVRDYSEVKGNGIITVDMAQQALEMLEVDQHGFDLMDRKLLLAVIEHF
                     NGGPVGIDSIAAAIGEEKGTIEDVLEPFLIQQGFLMRTPRGRIATSKAYQHFGFSAIE
                     QE"
     gene            complement(12479..12604)
                     /locus_tag="U6309_001810"
                     /pseudo
     CDS             complement(12479..12604)
                     /locus_tag="U6309_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172477.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
BASE COUNT         3750 a         2572 c         2400 g         4021 t
ORIGIN      
        1 aaatttatat ctaacaagta cggctaatag cagagccatg ttttatttta agtcagcaga
       61 tgatgtaaaa atagtccaat accataagag agagaggctg taaccgtgcc aattatcagt
      121 gtctcaaaac cggaatataa ccaggatttg gttgaccagt ggcttttgat tgaaccgatg
      181 acaaaaaatg taattgcagt gaaaaaacaa gaccagatga acgaattgtt catgaccagc
      241 acataaggga ttaatggaat aattccaaaa agtagaaacg cactaaaagt ataaagcgca
      301 gatttaagcg ggaatcgaag tgcagcaggc aacccatatt cttcttgtaa catggttttt
      361 atccataaag ctctgttcgc tgtaatttcc tcaacaactt gatctaatgt ttggccatgc
      421 aaacctttat tttgaaaaat ttgttttatt tcgttttttt caccttctgg aataaaatca
      481 atatgttttt cttcaattgt tttatagcgc tgaaattgat ctacctcaga tttggtaccc
      541 agataattac ttgctgccat tgaaaaacca tcagcaagca agttggcaaa acctaaaatt
      601 agaataacta aagaagataa ttgtccgccc acaacaccgg aaacaatggc aaaagtagtg
      661 acaacaccat caattcctcc atatatccaa tctcgcaagt aattttgaga tgggatctgt
      721 gctaatcgct ctctcatttc tttttgcgaa tgtccatgct ctaaattttc catagcagat
      781 cctttctcca tatgtgttat ttattaatta tcaattctgc caatattatc gctagtttga
      841 caaagcatat aataaaattt agtatatttg agtttgttag atggcgcggg gtgtcggaaa
      901 gtccggctga gagttacccg ttgaacttga tctgaatcat atcagcgtaa ggacgccttc
      961 aaaaaaacca tggccttttt aaggcatggt tcccttttgc catctccata atatttaaaa
     1021 ataataacat aaggagttat ggcgatgtca tcactaaaat ccagggttac cctactgctt
     1081 aattggtaca ccaaccctta tcataccccc atacttgtgg cacaacaatt agggttttat
     1141 tcagaagaag atatcaagct tgccattctg gaacccgcgg atcccagtga tgtaaccgaa
     1201 atcgttggac tgggaacagt agattttggt gttaaagcaa tgattcatac cgttgcagcc
     1261 aaagcaaaag gttatccagt tacttccata ggaacgcttc ttgatgagcc acctaccgga
     1321 cttattgctt taaaatcaag tggcatcaat tctttccagg atattgttgg taaacgagta
     1381 ggttatatcg gcgaattcgg caaaaaaatt attgatgatt tggccagcct cgcgggtatt
     1441 gatccgacaa gctacaaaac agtccgtatt ggcatgaatg tcacagatgc catttatcgg
     1501 gatattatcg atactggtat tggttttatt aattttcaaa aagttgaact ggagcatctt
     1561 tgcggagaaa ctgtattttt acgaatcgat caattagctg gtttaggctg ttgttgtttc
     1621 tgctcaatac aatttattgt tcctgaaaca acattacagc aacccgaatt agtcaaagga
     1681 tttctcagag caacacagcg cggtgcagcc tacacgacag aaaaacctga agaagcttat
     1741 gagttattat gtcaggctaa accacagttg cgtactcctt tatatcaaaa aatttttaca
     1801 agaacattac catttttttc acgtacatta attaatgttg acagagactg ggataaggtt
     1861 ggacgctata ccaagcatct aaaaattatt gatgaacatt ttgatatctc gcaatgctat
     1921 acgaatcgtt ttttgccaga tataccttat tctgatttga aaccaatcgc ctgttgtctt
     1981 gaaaattaat tatgcgagca ggtattgtag gttcagggat catggggcgt ctgctggctg
     2041 ttgcattaca taatgctggc tggcaggtat ccctttttga tcgctctaat gaaagcatga
     2101 gttgcagtac tgctgctgca ggtttattaa caccgttttc tgaattggat aaatcaattc
     2161 tattgatttc caccttgggt caagaatcta ttaagaactg ttggccacgt attattgagg
     2221 atttgcctga agctgtttat tttcaacaat cgggcagtct ggtgctatgc catccaggcg
     2281 atcaggcgga atggattcac tttagtcggc gtattctgag tcaactcaat ccgagtaatt
     2341 ctgtttttca aaaattatcc catgaaaatc tcattgcgct tgagcctgag ctgacaaagt
     2401 ttgaaactgc ttattatttt ccaaatgaag cacatattga ttgccaaaca ctaatgttgt
     2461 ctttaaaaaa ttatctcgac tctgtttgca tacctcacat taccaatact cctgtcttat
     2521 cgcttaaacc tggagagatc aatacaaaag aacaaagata tcattttgat gtcgttttcg
     2581 attgtcgtgg tttaggtgca caaaatatat tttctgattt acgaggtatt agaggtgaac
     2641 tcatttggct tcatgcgcca ggagttcaac tacatcgccc tattcgtctt ctgcatccaa
     2701 ggtacagtct atatcttgta cccagacctg gaaatcttta cctcataggc gctagcgaaa
     2761 ttgaggcaga agattttagt ccggtttcag tacgtacgac tttggagtta ttgaccgctg
     2821 tgtattattt acatgggaac tttgatgaag cacgcatcat aaaaacagtg actcattgcc
     2881 gtccaacttt agcaaatcat ttaccacgta ttaaatttgc cgacgggcta atagcagtta
     2941 acggtcttta tcgtcatggt tatttaatag ccccagcctt ggcagaagaa attttacgag
     3001 gtgtgacatc caatttccgt catctgaatt atccagaact ttgggagagt tattgtgatt
     3061 aatgtctatt tcaacgataa aatttataaa atccaaccct cattatcatt aagcgcattt
     3121 ttaaagcagc ttaattatac tcaacagcat tttgctgtcg ctataaataa tcatcatgta
     3181 ccacataaag cttatgaagc tacaattttg caagccaatg accgcgtcga tatcctcttt
     3241 cccatgcaag gagggtaatg atgtggaatt tggcaggaaa agaattaagt agccgcttac
     3301 tcttaggtac tgcgtgttat ccttcgttag agcatatgca gcaggcaatt cttaattcgg
     3361 gtacagaagt tattacgatc tcaataaaac gccagacttc tgcaggatta gatggaaaat
     3421 ctttttggca agcagtaaaa aaattagatt gtcatctttt gcccaatacg gctggttgcc
     3481 gtaatgcgga ggcagcgatc aatacagctg aaattgcgag ggagttattt gatactcact
     3541 ggataaaact ggaagtgata ggcgatgatt ataatttaca gcccgaacca tttgagttaa
     3601 tcaaggcaac cagaattttg attgatagag gatttgaggt ttttccttat tgcacagatg
     3661 atctggtttt atgtcaaaaa ctggttgatg caggttgtaa aattttaatg ccttgggggg
     3721 ctcctatagg ctcaggtaag ggtttaatta acccctatgc cttggaaact ttgcgttatc
     3781 gatttcctga catcacatta atcattgatg ctgggattgg taagccatct catgctgtac
     3841 aggcgatgga atttggtttt gatggcgtgc ttctaaatac tgctgtagct cttgccaatc
     3901 atcctgctct catggcaacc gcattccgcc atgccgttat tgcaggtcat caggcattta
     3961 caggaggaat gatgtctgag agaaatgtag ctcacccaag tactccatta atcgatactc
     4021 cattttggca tcaggtaaac aatttatgaa aaaaccgatt gtttggacaa ttgctggtat
     4081 tgactcatcc ggattagctg gtgtccatgc tgacatggaa acttttagtc gcttaaatgt
     4141 cagggcttgt tcagtcatca ccgcagtaac tgcacaaaat gctcactcaa ttacagcagt
     4201 agaagaaata tccagagatc aggttgctgc tcagtgccgt gccttggagt taaatttaaa
     4261 accggatgcc attaaaattg gtatgttatg ttctacacca atctgtgaag aaatagcttg
     4321 ttttttaaag ggttatgaag gatttgttgt tcttgatccg ataattacct cctcctcggg
     4381 aactaacctg tttttttcag atttacaaca gcataaaaaa aatctaattc aattatttcg
     4441 gtacgttact attattactc ctaatcgaat tgaagcagaa gctattttga atcggagcat
     4501 ttcttcttat caggatataa tcaatgctgc ttcagatctg ttgtcactgg gagcaaagca
     4561 ggtattactg aaaggaggac atctaaaaga taatttattc agtcaagatt actggaccga
     4621 tggaaaagag tcattttgga ttgcgaatca tcgctttcct gaaacaaatt accgtgggac
     4681 aggctgtgtt ctatcttctg cactcacagc ttgtcttgcg ctaggctatt caataaaaga
     4741 tgctattgtt attgctaaaa tgtatgtcaa tcgtggcata cgccaatcaa tagaaattga
     4801 taaagacgcc tcgcaacttt atcatgatgg ttggccagaa gatgaggccg atctccccta
     4861 tttatcacca acaccgttca ttaaaccagt tccttcattt aaaaaatatt gtatgggttt
     4921 ttatcccatt gtggatagta gccactggtt agaaatgctt ttgccattgg gagttaagtg
     4981 cattcagtta cgaataaagg aagcttctca agagcggttg gaagaagaga taaaacggag
     5041 tgtccatttg gcaaatcagt ataatgcttc attatttatt aatgattact gggaacttgc
     5101 tattcattat ggtgctgcag gagttcacct tggccaagag gatctggaaa aagctgatat
     5161 cgatagaatt aatcgatcag gtttattctt aggtataagt acgcattgct attacgaagt
     5221 tgctcgggct catgctctta acccttccta cgttgcttgc ggccccatat atgaaacaac
     5281 cagcaaaata atgccatttc aagcacaagg aattgcacga cttgaacgtt ggcgaaaaac
     5341 gcttcgttat ccattggttg ccattggggg tataacttta aagaaccttt ctgatgtctt
     5401 aaaaacaaaa attgatggtg tgtcagtgat ctctgccatt acaaaagcat cagcaccttt
     5461 agttgcagcc aaacaatttt taactcaaat gaatgaatca cataatgaat aaatcaaatt
     5521 tggcaatgaa cgaattaacc cgctattctc aacaaataaa gatggaagaa attggattag
     5581 acggacagga aaaacttaag aattctcgag tattatgcat tgggctgggc ggtttgggtt
     5641 cgcctctctt actttacctt gctgcagcag gtgtcggagt gttagggatt gtcgatgatg
     5701 acatcattga gctaagtaat ttgcataggc agattcttta taatcatacc catattaata
     5761 aaaaaaaggc cgttactgca aaaaagcaac ttctggctat taatccccta attcaagttg
     5821 aatcttattc cagcagatta acagaagaaa atgcggctga gttaattacg cagtatgaca
     5881 tcatcgcaga tggaaccgac aatttttata cgcgatattt gattcacagc atttgctttg
     5941 agctggaaaa accttatgtg tatgctagtg cctcaggttt ccaagggtat tgctctatat
     6001 tccacggtaa acaagatccc tgcctccatt gtatcttccc ggtttctgta aacgatatca
     6061 atatgcctaa ttgcgaggga gtcggggtaa ttggtacttt acccgggatg ctaggtatta
     6121 ttcaggcaac agaaataatc aaatggatac tacaaattgg caatccatta aaaaagcgtt
     6181 tgcttgctat taacttgtta acaatgacgt ttaagaacat cattatttca aaaaatccgg
     6241 attgtgaatt ctgcattcag agtcagtcaa ttagacaatt aattaatcct gttaattgtt
     6301 caactcaaat tgagccatgc catcatagaa taactcttga tgagttttat caattattta
     6361 cgaataataa aaacattcat ctaatcgatg ttcgtagtac aaaggaacat caaatacaaa
     6421 atataggagg tattgtgctt cctctagatg agctgcctca acgcttacat gaactaaacc
     6481 ctaatcggct tattattctg tactgccaat cgggaaaacg aagcaagatg gcattagata
     6541 tacttctcaa ggctggcttt gcttctgtta aatatataga aaatggttta aagggagtaa
     6601 ctgccaacta caataaagac atgccccaat gattcacccc tcctaaaaag gaagctgaaa
     6661 ttaaataagt ttatttttac cttcggcaat attatttagc agataagcga gtctacaccc
     6721 agccagaagt atctgttttt ctgtaatatt ttgagtattt aactgatatt gcttgcccgg
     6781 cacctgatga gctgaaacct tataaacttt agtcaatgcc aattgatgtg atgcattaat
     6841 ccattgttgt ggatttttgt ccttactggc tgattggcaa gaccatttct tttccaattg
     6901 tctcgccttg tttttaatct gaaaaaactt gtcttgacca atcagaatac cgccaccatt
     6961 atcccaatat tgatgcaaat tattcccaat aggattttta gctaactgaa ataaattacc
     7021 acccaaatca cctttaggta attttttact tatctttgta acggtatgca aaggctgatg
     7081 aatatctcca accaaatgca ccaatatgcg taagctcaat tttttatcag caatactggc
     7141 tttattggaa gacagcacag caattgcctg attaattccc cagagtgcat tgatgtctgg
     7201 taaggctggt aattctgttt catccataga aaaaggaatg tcaatgtaat gtaaagcatc
     7261 aaaccaatga acatcatgtg ctcttataga atccagccat gaagctgatt ttacaaaatt
     7321 gacattagac gacgtcttcg attttgaatg ggaatataaa tcacacattc gcctggattg
     7381 aggtgtcaaa ttatcatatg cgatctgggc taccagttgg tgccctatcg cgttccatgc
     7441 ataaccagca ttaacaataa ataaaaaaaa cgataccaat atgcttctta tcattcttaa
     7501 ctcaggtaag gggaccaagc gggctcttga acatctcctt cacgagcagg aagtctcatt
     7561 ctaattctcc cgtcaattga cacaatgcct aaaactcctc tgtcctgata acgggtagca
     7621 taaagcacta aacgactatt tggagatact gatggtgatt catcaaggcc agaaaaggtt
     7681 aaattggaaa tcgggccacc acctgtattt tgcacaccaa tattaaattg tctatcctcc
     7741 ctgtgcagca taacgatatg cttcatatct ggagtgtagg aggcacgggc attataattg
     7801 ccttcgaaag tgactcgact tatttggcca tcggccaatg ataagcgata tacttgaggt
     7861 gacccgcccc tccctgaagt gaacagcaaa ctgcgaccat caggagaata acgtggttca
     7921 gtatcgatag aattaccaaa agtaagctgt ctcatagaac cgctgctcaa atcaacatcg
     7981 taaattttgg gtgtaccgct tttggataat acaactgcca aatgccgacc gtcaggtgac
     8041 catgcaggcg ctccattaat tcctgggaag cttgtaatta agcgacgctg tcctgtttca
     8101 acggagacgg taaaaatttc agcttttttc ttttcaaaag aaacatagga tatcgacttt
     8161 ccattaggtg accatgaagg tgacataata ggctctgaag agacgagtaa gctttgagga
     8221 ttgtgtccgt cagcatcagc aacctccagt gagtagcgag accttcctcc gtttctttgt
     8281 accgaaatat aagctatcct ggtggagaat atcccccgtt ctccagtcag tttttggtat
     8341 acctcatcac taatatgatg agccaaagct cttacctgat tggcattgat ctgaaaggtt
     8401 tttgtcaaca aaatattgcc attggctacg gcatcagcca aagtaaagct cacttcaatc
     8461 cggttaccaa cctgattaac tcggccagtg actacactat cagcgccaag ttgacgtaat
     8521 gtactcacgg aagattgaga attcgcgcct tgtggtccag agataatttt aaattgtcct
     8581 gatatagtca aatcattctc tatgacattt cctatttcct gagccgccgc gtctgagcca
     8641 aaagaattga tggctatggg cagggctgaa ttaattcctt gagttagttc caaatcaaga
     8701 gcaatcactt gaccggtaaa taacagcaag aaaagtgaaa taattcgatt gaacacgagc
     8761 tttaccccct aacttgttct ggacgaacgg ttaaactgat atcgcgaaac aaattaaatg
     8821 tttcaacgtc ttgaggcacg ggaagtggtg aagccttgta tattgctgtt tgtgctgacc
     8881 tgtctaacaa aggatcacca ctgctgcgga ttagagacac ttccagtaca gttccatcag
     8941 gagcaaggcg tatcctgaat tggctcgaca gagaactgtc gacattttca ggtaaaatcc
     9001 agttgcgtcc gatcgcatta acaataagtg ccttgtattt atcaacttca ccagctatcc
     9061 gtgcttgtct ttcagcattt tgggctgcct gttgggctgc agccgcttct gcttgtcttt
     9121 ttctttccat ctctgctttt tcttgctcag cacgggcttt ttccgctttc agcttttcag
     9181 ccttcgcctg ttcagcttta agtttctcgg ctttctcttt ttcttcttgt tttttcttat
     9241 tgagttcagc aagtttttta gcttccaatt cccgttgttt ggccaacttt tcgttttgtt
     9301 tctttaactc ttcaatacgt ttcgcttcta atgctttttg ctcagccaat tcttttaacc
     9361 gttttttttc ttcttcaact tgtttttttc tggcaattgc aattttatta gcttcttctt
     9421 tcaatcttgc aatttcttgt tgctctttga ttctttgctg ttttgcagcc tcagcttgcc
     9481 ttaataattc cttttgtcga ttaagttcag ctttcttctg ttgttcacgt tcttgtttca
     9541 aacgattaac ggttttcatt atttctttat tgtcgacact gaccgctttt actacttcct
     9601 gctgagaagc agtaggttgc atcatgccag gggaattttt ggattccatg gttagcacag
     9661 gtcttttgct gctattatct gtaaaaagca atacaagtaa aaaaagatga agtgtgacag
     9721 caaaaagaaa tgcttttcta tagctaagat cacttatcat gcttgcccct catcaccagt
     9781 ctgagagtca gtaagcaatc ccacttgctc agctcctgct tgctttaaga gtgccatggc
     9841 ttgaacaact ttcccataag caaccccctg atctccctta acaagcacat tgagtttttg
     9901 acctgactgt ttggctagct ccagttctgc cgcaactcgt actaataatg cctgagcttc
     9961 aataggttct gctggtgtac tactaatatt taaaaaatag ctgcctaatt gattcactga
    10021 cacaatgata ggttctctgt cagcagcctg taaagtttga ctggctgctt tgggcagatc
    10081 tactgtaaca ccttgtgtca gcattggagc agtaatcata aaaataacca gtaaaaccaa
    10141 cataacatca atgtaaggca ctacattaat ttctgagatt ggacgttcac gattggtttt
    10201 agatcgattc attttaagtc ttatcctcta ggtgaaccag aagtttgttg ttcaattaaa
    10261 gaaatcagtt cttcttgaaa taaatcatat ttattcagca aagtattggc acgtgtcgta
    10321 tatcggttgt aggcaataac cgcaggaatt gcagtaaata gtcctaaagc ggtagcaacc
    10381 agcgcttctg aaatacctgg tgccaccatt gcgatagtgg cctgttgggc atgtcccaaa
    10441 gcctgaaatg atgtcattat accccaaact gtgccaaata gacccacgta gggagaaata
    10501 gatcctacag atgcaaaaaa tggtaaatgt tgctccagtt tttccgcttc tttggcatga
    10561 cttatctgca ttactctttg tataggctca atagatactt tgccttgttt tctgcagcga
    10621 acaaattctt taaaaccagc gtgaaagata gcggctaccc cttgtctttc atcactatta
    10681 ctgtctacat cagcatagag tttgctcaaa tctccacaat cccagaacct tctggtaaag
    10741 gtttcagtca tttgctttct acgattaaag aaccatgctc tttgaaagat taatgtccat
    10801 gaaattatcg aagcaattaa taatatcaac ataacggact ttactaccaa acccgcttgc
    10861 ataaaataca ttagtacatt tgcttgatta gccaccatta atctccacac tgaaaggttt
    10921 cgttattcat ttctcataat attaggcagt cgcttgggct ttaaattact gccaacgcaa
    10981 actacctgaa cactggccct gcaaattaat tgtttatgtt gattagatat ctcttggtta
    11041 aatagaaaac tacacgctct aaattcttta atttctgttt taattgttaa ttgatcatct
    11101 aacctggccg gaaatacata attaattgtg agttcgtgaa tcgcaaataa aacatcggac
    11161 ttttcgaatt cacttagaat taaattgttt tgccttaata attcagttcg agctctttcc
    11221 aaataacaaa ggtagttagc atgataaaca attcccatat aatcaacatc ttctgcatag
    11281 actctaatgt tatgctgatg atttgatata agattcatgt gttattcctg ttcgatcgca
    11341 ctaaagccaa aatgctgata agctttcgaa gtggctattc taccgcgagg agtgcgcatt
    11401 agaaagcctt gttgtattaa aaatggctct aaaacatctt ctatagtccc tttttcttcg
    11461 ccaatagcag ctgcaatact atctatgcct actgggccac cattgaagtg ttctatcacc
    11521 gctaacaata attttctatc cattaaatca aacccgtgct gatccacttc aagcatttcc
    11581 aatgcttgtt gggccatatc aacagtaatg atgccatttc ctttcacttc agaataatcc
    11641 ctgactcttc ttaacaggcg attggctatt cgtggtgttc ctctggagcg taatgcaatt
    11701 tctctggctc cttcgggttt tgtagggaca ccaagcaaat gcgcagagcg tgccacaatc
    11761 ttggtaagcg aatcaacaga ataatattcc aaacgttgca ctataccaaa tctatctctt
    11821 aatggcgagg taagcaaccc tgctctcgtc gttgctccaa ttaatgtaaa gggaggtaac
    11881 tccaatttaa tagatcgtgc tgctggcccc tctcctatca tgatatccaa cttataatct
    11941 tccatggctg gataaagaat ttcttctata acaggactta atctatgaat ttcatcaata
    12001 aatagtacat cattttgttg tagattcgtc aatatagcag caatatctcc agcacgctca
    12061 atcactggcc cagaagtttg tcggatattc actcccattt cgtgagctat gatatttgcc
    12121 agagtggttt taccaaggcc aggtgggcca aaaataagaa catgatccaa aggatcgttt
    12181 ctctttcttg ccgcattgat aaatatctgc atctgtgagc taacagaatc ttgccctacg
    12241 tactcgctca agctaagcgg cctgatggct ctatccattg cgtcttccga gacaatactt
    12301 tgactactta ttaggcgatc actttcgagc ataagaggtt ttatcgggtt cattcgaatc
    12361 aaagcatttt aacataggtt tatctaatag cagatctttg aaagcttatt tatttaataa
    12421 ttaaatgcaa ttgatttcac gattaaggtt gatgatttta tgtgatatca aaatgatatt
    12481 ataaagtgtg attctcactt acaatatcca tcagctgttt tcgagctcta aatatacgtg
    12541 agcgtacagt tccgatgggg caatgcattt cttttgcaat atcctgataa gtttgtccat
    12601 caaatatgtg cagcccataa cataatcgta actcttcaga taaacatgac attgctgctt
    12661 ctatttgttt ctcaaactct aatcccatta tgtctgactc tggagaatta tgtggtgtag
    12721 aaaattgttc atccatatac tgc
//
LOCUS       DASLOE010000084        12527 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.084, whole genome shotgun sequence.
ACCESSION   DASLOE010000084 DASLOE010000000
VERSION     DASLOE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12527
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.084"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            94..720
                     /gene="lvgA"
                     /locus_tag="U6309_001811"
     CDS             94..720
                     /gene="lvgA"
                     /locus_tag="U6309_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LvgA"
                     /protein_id="HEN5505250.1"
                     /translation="MADGDIEIKAGFVDTDLDDRKLTMIDDLNNPLAIVERVYLIWWH
                     WADFHLHVISPHIDTITPAIVIEPELIPGSNDHEFVYSIHDSGSKLSTSKSQDMFSAG
                     MSMCKLFYTIEKMVHILVDRLKSGGVSMEEEVQIAFAGHEIAQRKAFESIINLPYNVV
                     VTNFDPGIWGEKYLQNVKRLADKGYGYPPESPRENYKHPVSSATTARK"
     gene            complement(750..1127)
                     /locus_tag="U6309_001812"
     CDS             complement(750..1127)
                     /locus_tag="U6309_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN5505251.1"
                     /translation="MFFIKYTGALIYDLIILFILFFAYTSVLLLFTHGQAIPPATRWY
                     QFSLFIITLVYYYGSIRCGGQTVGMKAWRFKLTTDSNKTLSRQQIIARFFYFIPATLI
                     APFCLKGNYVLLHQWTQTHFKKV"
     gene            complement(1277..2416)
                     /locus_tag="U6309_001813"
     CDS             complement(1277..2416)
                     /locus_tag="U6309_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEN5505252.1"
                     /translation="MIAKRPIYNQQLKCVAFEILSCRNVILNEELTRQFYELIYHTDT
                     QLPLFVPFSLKKLLEQFETPINNPIILKLSADEIESVYSLTELQDSLFSIALLINTPQ
                     QLAWLNFAEYIGLTEQLMSKSDVTKVVQYSKAKNRKIIAYDLNQHVNFEKCKAMTMDY
                     YCGDFLLNYIAEEHTEIAANKLNVLQLIQELQKEDCDLNTITHIIQEDPLLSYQLLKL
                     VNSVGFSSGPSIESIDQAVARLGIINLKKWVMLFSMKNISDKPVEILESGLIRAHMAE
                     ELAKKSSNIFSQSAYTAGLLSILDCLLNKPMPALVEQITLAEEIKNALTNKSGPLGNL
                     LSLVIAYEEGHWEEVTQLNINGIDLSQLYIDSLALVSQGTKIMNN"
     gene            2737..3111
                     /gene="sdhC"
                     /locus_tag="U6309_001814"
     CDS             2737..3111
                     /gene="sdhC"
                     /locus_tag="U6309_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEN5505253.1"
                     /translation="MNKKRPINLDLSSLKFPPMAIASILHRVSGVLIFLLFPFVLFIL
                     GCSLQSEESFAQTKIILANPYYKLLLWAFGASMIYHVIAGIRHLIMDMGFGESLRAGR
                     MSAVSIIALAVILAIFLGIWIW"
     gene            3105..3452
                     /gene="sdhD"
                     /locus_tag="U6309_001815"
     CDS             3105..3452
                     /gene="sdhD"
                     /locus_tag="U6309_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEN5505254.1"
                     /translation="MVNNVTSLTGNGLKDWLIQRVTAVYFAAYLIFLFGFILANPDLN
                     FSQWHLLFANKVFQIATTIGLMALTLHAWIGIWTVTTDYMKCTIIRLSVQLLVVLWLL
                     GQFIWGLMIIWGQ"
     gene            3454..5223
                     /gene="sdhA"
                     /locus_tag="U6309_001816"
     CDS             3454..5223
                     /gene="sdhA"
                     /locus_tag="U6309_001816"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEN5505255.1"
                     /translation="MTVARNRFDAVIIGAGGAGMRAALQMTNSGLKVALLSKVFPTRS
                     HTVSAQGGITAALGNADEDDWRWHMYDTVKGADYIGDQDCIEYLCKTGPEAVYELEHM
                     GLPFSRMDNGKIYQRQFGGQSKHFGGEQAARTCAAADRTGHALLHTLYQQNLKAKTHV
                     FSEWYALDLVKNSQGRIAGVTAMCMETGEIVFFQTRVCILATGGAGRIFQSTTNAFIN
                     TGDGFGMALRAGIPLQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLINKDGERFMERY
                     APRVKDLASRDVVARAMALEIRAGKGFDPKGVDHVKLKLDHLGADLIMSRLPGIRELS
                     MKFAGVDPIVEPIPVVPTCHYSMGGIPTNMHGQVITKTDGKEHVVEGLYAVGECACVS
                     VHGANRLGGNSLLDLVVFGRAAGIHVEELWQSNQLPDMSYVSEDDVVASLVRYERWEN
                     SKDGESHAVIRDEMQRVMQEDFGVFRTGEVMASGLKRLQALRDRLAHAKLDDKSQIFN
                     TERVEAMELDNLMATAYATALSAIARTESRGAHSREDYPKRDDANWIKHTLYFEEGEV
                     IDYRPVNTSPKYVEPFEPKERVY"
     gene            5236..5958
                     /locus_tag="U6309_001817"
     CDS             5236..5958
                     /locus_tag="U6309_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEN5505256.1"
                     /translation="MADSRNVILSIYRYNPEVDAKPYMKDYEIEIPAKSDPMLLTLLE
                     RLKAEQDPSITYRRSCREGVCGSDGMNINGTNGLACITHLSQLKTDKIVIRPLPGFPV
                     IRDLAVDMTQFYQQYERIEPYLQNDEVAPARERLQSPEERAQLDGLYECILCACCTSS
                     CPSYWWNPDKFVGPAGLLQARRFLADSRDKATQHRLDLLQDPFSVFRCRTIMNCTNVC
                     PKGLNPTQAIAKIRTQMLTQET"
     gene            6016..8826
                     /locus_tag="U6309_001818"
     CDS             6016..8826
                     /locus_tag="U6309_001818"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEN5505257.1"
                     /translation="MSSSDLQKEWASSYLSGGSMAYVDSLYEDYLADPDSVSEDWRAV
                     FSALPKVNGATKEVSHRDIRDYFLQNADKKLNKIIQIADSQQYQVASLINDFRSLGHL
                     AAKLDPLEMTERMPVPRLELAYHNLADVDVNRTFFAGTSFNGPEMTLGEIYNALRETY
                     CRSIGIEYMHISDTEVTEWLQHKMESVRGCPEFSQQEKLNILKDLIAADGLERYLGTR
                     YVGQKRFSLEGGDSLIPMMKELIRRSGVNQVKELVIGMAHRGRLNVLVNVLGKEPGLL
                     FQEFEGKIKYERTGDVKYHLGFSSDIKTESGAIVHLALAFNPSHLEIIGPVVEGSVRS
                     RLGRRNDLAKKDKVVPIVIHGDAAFAGQGVVMETFNFSQARGYCTGGTIHIVINNQIG
                     FTTSNPLDARSTLYCTDVAKMVQAPVIHVNGDDPEAVVFATQVAFDFRMKFKRDIVID
                     LVCYRRHGHNEADEPSVTQPKMYKKIKSMPTLREKYGEQLVSANLLTKTELDKLVDSY
                     REALDKGKAVVDLVHEDYEGKQSLDWTPYINAKWTDKVDTTISKEELEKISSQLNKLP
                     DGMKLHPVVERLLAERNKMTAGEIPMNWGYAEIMAYASLLHEGYGVRISGQDSGRGTF
                     AHRHAALHDIEKGDTFIPLEHISTNPKRYFTVIDSVLSEEAVLAFEYGFASSEPSFLV
                     IWEAQFGDFANGAQVVIDQFISSGEQKWGRLCGLVMLLPHGYEGQGPEHSSARLERYM
                     QLCAQHNIQVCTPTTPAQIFHLLRRQVIRNFRKPLIVMTPKSLLRHKLAVSPLEALFK
                     GKFHTVIPEIDALDAQKVTKVVLCCGKVYYDLLQMRRDKNLNHVAIVRIEQLYPFPKK
                     ALMAELEKYQKANEVVWCQEEPQNQGVWFSSQHNIRDCLRSDQTLQYAGREFAAAPAV
                     GSPGLHAEQQVALVEQALLGKK"
     gene            8862..10091
                     /gene="odhB"
                     /locus_tag="U6309_001819"
     CDS             8862..10091
                     /gene="odhB"
                     /locus_tag="U6309_001819"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEN5505258.1"
                     /translation="MSIEVKVPVLPESVADATVAAWHKKVGDKVSRDENLVDLETDKV
                     VLEVPSPVDGVLSDILFNTGDTVGSGDLLAKISQSVSVESQKTEKEEKPAKKEEIKIS
                     ESESVSAKEDKSTSPVVRRMMAENDLKAGQIQGTGKDGRITKEDVLSYIESNREKSNQ
                     TAEVQKESSMRALSFGGREEKRVPMTRLRAKIAERLLQAQHNAAMLTTFNEVNLKAVM
                     DMRSQYKDSFEKKHGVKLGFMSFFTKAVVESLKRFPAVNASIDGQDIVYHGFYDIGIA
                     VSTERGLVVPVIRDADQLSMAEIELAINDAASKARTGKLSMEEMQGGTFTITNGGVFG
                     SLLATPIINPPQTGILGMHKIEERPIVEKGQIVIRPMMYVALSYDHRLIDGKDSVQFL
                     VSVKELLEDPSRLLLNV"
     gene            10154..11317
                     /gene="sucC"
                     /locus_tag="U6309_001820"
     CDS             10154..11317
                     /gene="sucC"
                     /locus_tag="U6309_001820"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEN5505259.1"
                     /translation="MNLHEYQAKQLFASYGLPVPRGEVAYNVEDALLVASQLSTSRWV
                     VKAQVHAGGRGKAGGVKLVSSKDELAAVAKSMLGTRLVTYQTDARGQPVNAILVEETC
                     EIDKELYLGAVVDRSTRRVVIMASTEGGVEIEKVAHETPEKIFKVVVDPLVGVMPFQC
                     RETAFKLGLKDDQIKQFTHLMMGLGKMFVDCDLSLLEINPLVITKSGQLICLDGKINI
                     DGNALFRQPKLKNMRDVSQEDDRENRASDWELNYIPLDGTIGCMVNGAGLAMATMDVI
                     KLHGGEPANFLDVGGGATKERVSEALKIIVSDEKVKGILVNIFGGIVRCDLIADGILA
                     AVKEVDVKIPVVVRLEGNNAQLGAEILNKSNLNVIAATSLTDAAKKIVAAVSE"
     gene            11392..12267
                     /gene="sucD"
                     /locus_tag="U6309_001821"
     CDS             11392..12267
                     /gene="sucD"
                     /locus_tag="U6309_001821"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEN5505260.1"
                     /translation="MSVLVDKHTKVLCQGFTGKQGTYHTEQALAYGTKMVGGVTPGKG
                     GQMHLGLPVFNTMREAVEETQADASVIYVPAPFCKDSIIEAAEAGVKLIVCITEGVPV
                     LDMLQVKVYIENNTQARLIGPNCPGIITPGECKIGIMPGYIHLPGKVGIVSRSGTLTY
                     EAVKQTTDKGFGQSTCIGIGGDPIPGTSFIDALSLFEKDPKTEAIIMVGEIGGSAEEE
                     AAEFIKSHVKKPVVSYIAGVTAPAGKRMGHAGAIISGGKGTAAEKYAALEAAGVKTVR
                     SPADLGDAIAEVTGW"
BASE COUNT         3789 a         2088 c         2829 g         3821 t
ORIGIN      
        1 gcttttttac cttttcttaa ggtaggttta atgttctatt gtcaattttg ggctaatata
       61 acaataatat ccttactacc cgggtaaata taaatggcag acggcgatat cgaaatcaaa
      121 gcaggtttcg tagatactga tctagacgat cgcaaactga caatgataga tgatttgaat
      181 aatccattag cgatagtgga gcgagtctat ctaatatggt ggcattgggc agattttcat
      241 cttcatgtta tttctccgca tattgatact attactcctg cgatagtcat cgaacccgaa
      301 ctaatacctg gttctaatga tcatgaattt gtttattcca ttcatgatag tggctctaaa
      361 ttatctacat ccaaaagcca ggatatgttt agtgcgggca tgtctatgtg caaacttttt
      421 tatactattg aaaaaatggt tcatattctg gtagatcgct taaaatctgg cggtgtcagt
      481 atggaggaag aggttcagat agctttcgct ggccatgaaa tagcccagag aaaggccttt
      541 gagtcgatta ttaatttacc ttataacgtt gttgtgacta attttgatcc aggaatatgg
      601 ggtgaaaaat atttacaaaa tgtgaagcgt ctggcggata aagggtatgg ttatcctcct
      661 gagtccccaa gagaaaatta taagcatccg gtatcttccg caactactgc acgaaaataa
      721 ataattagcg agaatggctc aacagtgttt taaacttttt taaaatgcgt ttgggtccat
      781 tgatgcaaaa gcacataatt cccctttaag caaaaaggtg ctattaaggt ggcaggaata
      841 aaataaaaaa atcgggcgat aatttgttgt cttgatagtg ttttattaga atcagttgtg
      901 agtttaaatc tccaggcttt catacctaca gtttgtccac cacagcgaat agaaccataa
      961 taataaacaa gagtgataat gaataatgaa aattgatacc aacgggtggc aggaggtatg
     1021 gcttgtccat gagtgaataa tagcaggaca gaggtgtatg caaaaaataa aatgaacaag
     1081 atgattaaat cataaatcaa tgcacctgtg tatttgataa aaaacataac taatacatat
     1141 cctttattga tttcaactat taaattttgg aatattgatc atttaggaaa acacattcta
     1201 aattttttgt tgctcaaaga gaagcctggg tcaggcatag aaaaataaaa agaatattgt
     1261 ttaaattgtc gcacaatcag ttattcatga tttttgtacc ttggcttacc aaggctaaac
     1321 tatcaatata taattgactt aaatcaattc cattgatgtt aagttgagtt acttcttccc
     1381 aatgtccttc ttcataagca attaccaaag acaatagatt tcctaatggt cctgatttat
     1441 ttgtcagagc attttttatc tcctcagcca gggtgatttg ttctaccaat gctggcatgg
     1501 gtttgtttaa taagcagtct aatatggata ataatcctgc agtataggca ctttggctga
     1561 aaatattcga acttttttta gcgagctctt ccgccatatg ggctctgatt agacctgatt
     1621 ccaatatttc tacgggttta tcagagatat ttttcatgga aaacagcata acccattttt
     1681 tcaggttaat gatgccaagc ctggcaacgg cttgatcgat ggattctatg cttggtccac
     1741 tagaaaatcc aactgaattg accagtttta gaagttgata gcttagtaat ggatcttctt
     1801 ggatgatatg agtgatcgtg tttaaatcgc aatcttcttt ttgtaattcc tggatgagtt
     1861 gaagtacatt taatttgttt gctgctattt ctgtgtgctc ctctgcaatg tagttaagca
     1921 ggaaatcgcc acagtaataa tccatggtca tagccttaca tttttcaaaa ttaacgtgtt
     1981 gatttagatc ataagcgatg attttacgat tttttgcctt actgtattgt actaccttgg
     2041 ttacatccga tttactcatt aactgctcag tcaatcctat gtattcggca aaatttagcc
     2101 aagccaattg ttgaggggta ttaatcagta aggcaataga aaatagagag tcttgtaact
     2161 cagttagtga ataaaccgat tcgatttcat ctgcggataa ttttaaaata atggggttgt
     2221 tgattggcgt ctcaaattgc tcaagcaatt tttttaggga aaaaggaaca aataaaggta
     2281 attgggtgtc tgtgtggtaa atgagctcat aaaattgcct tgttaattct tcattaagta
     2341 ttacattgcg acaggataat atttcaaagg cgacacattt taattgttga ttatatattg
     2401 gtcttttggc gatcataaag acctcctttt gtccacatta tgtaatcata gtacgggttt
     2461 ttgcaattgg ccaatggact attcattttc cttataaact gaactttgcg tgataaaaac
     2521 agagtgctat acttcccatg catgaattga aatttagtaa ttctaaatag tttttaagac
     2581 ccaatttttt atttttcaaa gccatttgtt gcgcagtata cttaatgaat tatgtttctc
     2641 aataagtcta aagggtgaat gtgaaataat tgaatgggat tcaatattta gaagaaaata
     2701 tattgatgac attttcttat gaggaagaaa ttaatagtga acaagaaaag accgatcaat
     2761 cttgatttaa gcagtttgaa atttccgcct atggctatcg catcgatatt acatagggtt
     2821 tcaggagttc tcatattttt attatttccc ttcgtattgt ttattcttgg ttgttccttg
     2881 caatcagagg aatcttttgc acagactaaa ataattttag ctaatcccta ctataaactt
     2941 ctgttatggg cttttggcgc ctctatgatt tatcatgtca tcgcaggtat aagacatctg
     3001 ataatggata tgggatttgg tgagagtctt cgtgctggtc gtatgtcggc cgtttcaatt
     3061 atagctcttg cagttatttt ggcaattttt ctaggaatct ggatatggta aacaatgtca
     3121 ccagcttaac aggaaatggg ttaaaggatt ggttaattca aagagttact gctgtctatt
     3181 ttgcagctta tttaattttt ttatttggat ttatattggc aaatcctgat ttaaattttt
     3241 ctcaatggca tcttttgttt gctaataagg tatttcaaat tgcaacaaca attgggttga
     3301 tggctttaac attgcatgcc tggataggta tttggacagt aactacagat tatatgaaat
     3361 gtactattat acgtttatca gtacagttgt tggttgtttt atggctcctt ggtcaattta
     3421 tctggggcct tatgatcatt tggggacaat agcatgacag tggcacgtaa cagatttgat
     3481 gcggtaatta taggagctgg cggtgcagga atgcgagctg cactccagat gacaaattct
     3541 ggtttaaaag tagcgttgtt atcaaaagta tttccaaccc gctcacatac tgtgtcggct
     3601 caaggtggta ttacagctgc tttgggaaat gctgatgaag acgactggcg ctggcacatg
     3661 tatgataccg tgaaaggtgc tgattatatt ggtgatcagg attgtataga gtatctatgt
     3721 aaaaccgggc ctgaagcagt ttatgaactg gagcatatgg ggctaccttt ttcacgaatg
     3781 gataatggaa aaatctacca acgtcagttt ggtggccagt caaaacattt cggtggagaa
     3841 caagcggccc gtacctgtgc tgcggcagat agaacagggc atgctttgct gcacactctt
     3901 tatcaacaga atttaaaagc taaaactcat gtatttagtg aatggtatgc tctcgatctg
     3961 gtaaaaaact cgcaaggaag aattgcaggt gttactgcca tgtgtatgga gactggcgag
     4021 attgtatttt ttcagactcg agtttgtatt ttggcgactg gaggagccgg tagaattttt
     4081 caatctacaa cgaatgcttt tattaatact ggcgatggct ttggaatggc actaagagct
     4141 ggtatacctc ttcaagacat ggaaatgtgg caatttcatc caactggtat agcgggagct
     4201 ggtgttctgg ttacagaggg ttgccgaggt gaaggtggtt atttaataaa caaagatggt
     4261 gagcgtttta tggagcgtta tgctcccaga gtaaaggact tggcttcccg ggatgtggtc
     4321 gctcgcgcta tggctttgga aattcgggct ggaaaaggat ttgatccaaa aggtgtcgat
     4381 catgttaagt taaagcttga ccatctgggc gctgatttga ttatgtctcg cctgcctggc
     4441 attcgcgagt tatcaatgaa gtttgccggt gtggatccaa tcgttgagcc gattccagtt
     4501 gttccaactt gccactacag tatgggagga atacccacca acatgcatgg ccaggttata
     4561 actaaaactg atggcaaaga gcatgtggtg gaggggttgt atgctgtagg ggaatgtgcc
     4621 tgcgtttcgg tgcatggtgc caatcgatta ggtggtaatt ccttattgga tttggttgtg
     4681 tttggccgtg cagccgggat acatgttgaa gaattatggc aatcaaatca attgcctgat
     4741 atgtcttatg taagtgaaga cgatgtagtg gcttccctgg ttcgttatga gcgttgggaa
     4801 aactcaaagg atggtgaaag ccatgcggta atacgagatg aaatgcaaag agtaatgcag
     4861 gaagattttg gagtattccg taccggtgag gtaatggctt caggattgaa acgtttacaa
     4921 gccctgcgcg atcgattagc acatgctaaa cttgatgata aaagtcaaat ttttaataca
     4981 gaacgcgttg aagctatgga attggataat ttaatggcaa ctgcttatgc aacggcttta
     5041 tctgcaatcg cgcgaactga aagcagagga gcgcacagca gggaagatta tccaaaacga
     5101 gatgatgcaa actggattaa gcacacattg tattttgagg aaggtgaggt gatagattat
     5161 cgtccggtta atacctcacc taagtatgtc gagccttttg agccgaaaga acgcgtttac
     5221 taatgaggat attttatggc tgatagtaga aatgtaatcc tgtcgattta tcgctataat
     5281 cctgaggtgg atgccaagcc ttacatgaaa gattatgaga tagaaatccc ggctaaaagt
     5341 gatcccatgt tacttacctt gcttgagcgc ttgaaagctg agcaagaccc ttctattact
     5401 tacagacggt catgccgaga aggagtatgt ggctctgatg gtatgaatat caatgggact
     5461 aatggattag cttgcattac acatctttca caattaaaaa cagataaaat tgtcattcgt
     5521 cctttgccag gttttccagt aattcgtgac ttggcagttg atatgacaca attttatcag
     5581 cagtatgaac gcattgagcc ttatttacag aatgatgaag tagcacctgc tcgagagagg
     5641 ttacaatctc ctgaagagcg ggcacaattg gatggcttgt atgagtgcat tttatgcgct
     5701 tgctgtacca gttcatgtcc ttcttattgg tggaatcctg ataaatttgt aggccctgcg
     5761 ggacttttac aagctaggcg ttttttagca gacagtcgtg acaaggcaac gcaacatcgt
     5821 ttggatttat tacaagatcc tttcagtgta tttcgttgtc gtactattat gaattgcacg
     5881 aatgtatgcc cgaagggact caatccaacg caagcgattg caaaaattcg cacgcaaatg
     5941 ttgacgcagg aaacgtaata aatcagtcct tgttactcag aaagtttctg agtgaaccct
     6001 ttggagagaa tgataatgag cagttctgat ctgcaaaaag aatgggcttc ttcctatttg
     6061 tctggaggaa gtatggctta tgttgatagt ctttatgagg attacctcgc agaccctgat
     6121 tcagtgtcag aggattggag agcggtattt agcgctttac ccaaagtaaa tggtgctacc
     6181 aaagaagtat ctcataggga cattcgcgat tattttttgc agaatgctga caagaagctt
     6241 aataaaataa ttcaaattgc agacagtcaa cagtaccagg tcgccagttt gattaatgat
     6301 tttagatcat tagggcatct tgccgcaaaa ctggacccat tggaaatgac ggaacgcatg
     6361 ccagtaccgc ggctggagct tgcttatcat aatcttgccg atgttgatgt taatcgcact
     6421 ttttttgctg gtaccagctt taatgggccg gagatgactt tgggggaaat atacaacgcc
     6481 cttcgcgaaa catactgtcg cagtataggc attgagtata tgcatatatc cgatactgaa
     6541 gtcactgaat ggttacaaca taagatggaa tcagttcgtg gctgtcctga atttagtcag
     6601 caggaaaaat tgaatatcct taaagatttg atagcagcag acggtttgga gcgttatcta
     6661 ggaacccgat atgttggtca aaaacgattt tcgcttgagg ggggagattc tttaattcca
     6721 atgatgaaag aactcattag aagatctggt gtaaatcagg ttaaagagct tgtcattgga
     6781 atggcacata gagggcgctt aaatgtgtta gtgaatgttc tcggtaaaga accaggacta
     6841 ttatttcaag aatttgaagg aaaaattaaa tacgaacgaa ctggcgatgt gaaatatcat
     6901 ctgggttttt cctctgacat aaaaacagaa tcaggtgcta ttgtgcactt ggctttggcg
     6961 tttaaccctt cacacttgga aattattggg ccagtagttg agggatccgt acgttcacgt
     7021 ttgggaagac gcaacgattt ggctaaaaaa gataaagtag ttcccattgt cattcatggt
     7081 gatgctgctt ttgcaggtca gggcgtggtt atggaaacct ttaacttctc ccaggcacgt
     7141 ggttattgta ctggtggaac gatccatatt gttatcaaca atcagatagg atttacaacg
     7201 agcaatcctt tggatgcccg ttcaactctc tattgtacag acgttgccaa aatggttcag
     7261 gcaccagtta ttcatgtcaa tggagatgat cctgaggcag tagttttcgc tactcaagtc
     7321 gcttttgatt tccgaatgaa gtttaaacga gacatagtaa ttgaccttgt ctgttacaga
     7381 cgtcatggcc acaacgaagc tgatgaacct tcagtgaccc agccaaagat gtataaaaaa
     7441 atcaaatcca tgcctacatt acgtgaaaaa tatggagaac aattggtcag tgctaattta
     7501 ttaactaaaa cagaattaga caaattggtc gattcttaca gagaagcact agataaaggc
     7561 aaggcggttg tcgatttggt tcatgaagat tatgaaggaa agcaatcttt agattggact
     7621 ccttatataa atgccaagtg gacggataaa gtggatacaa ccattagtaa ggaagaattg
     7681 gaaaaaattt catcccaatt aaacaaattg cctgatggaa tgaaactgca tcccgttgtg
     7741 gagcgtttgt tagccgaacg aaataaaatg accgctggtg aaatccctat gaattggggc
     7801 tatgctgaga tcatggctta tgccagttta ttgcatgaag gctatggcgt aagaatttct
     7861 ggacaagata gtggcagagg aacttttgcc catagacatg ctgcactcca tgatattgaa
     7921 aaaggcgata cttttattcc tttggaacat atatcaacca atcccaagcg ctattttact
     7981 gtgatagatt cagttctttc cgaagaagcg gtattagctt tcgaatatgg ctttgcttcg
     8041 tctgagccat ctttcctggt tatttgggaa gctcaatttg gtgattttgc aaatggagca
     8101 caagttgtta tcgatcaatt tataagttcc ggtgagcaaa agtgggggcg gctgtgcgga
     8161 ttggtgatgc tattacccca tggctatgaa ggacaaggcc ctgagcactc ttctgcccga
     8221 ttggagcgtt atatgcagct ttgtgcacag cacaatattc aagtatgcac tccaacaact
     8281 ccagcacaaa tatttcattt gttaagaaga caagttattc gtaatttcag gaagccgtta
     8341 attgtcatga cccccaaaag tcttttacga cataaattgg ctgtttcacc tttggaagct
     8401 ttgtttaaag gaaagtttca tacagtaatt ccggaaatcg atgccttgga cgcacaaaaa
     8461 gtaacaaagg tggtgttatg ctgtggtaag gtttattatg atttattgca aatgcgccgc
     8521 gataaaaact taaaccacgt agcgattgtc cgtatagagc aattgtatcc atttcccaaa
     8581 aaagcattaa tggctgaatt ggagaaatat caaaaggcta atgaagttgt ctggtgtcag
     8641 gaagagccac aaaaccaagg agtctggttc tcttctcagc ataatatacg tgattgcttg
     8701 cgttctgacc aaaccttgca atatgctggc agagagtttg cagctgcacc tgcagttggt
     8761 agtcccgggt tgcatgcgga gcaacaagtt gctttggttg agcaagcatt acttggtaaa
     8821 aaataaatct aaacaatata atattaagag aaggtataac catgtctatt gaagtcaaag
     8881 tacctgttct acccgagtca gtagctgatg caacagtggc tgcgtggcat aagaaagtgg
     8941 gtgataaagt atctcgcgat gagaacctag ttgatctgga aacagataaa gttgtgcttg
     9001 aagttccatc acctgtggat ggggtattgt ctgacattct gtttaatacc ggagatacgg
     9061 ttggttcagg agacttactg gcaaaaattt ctcaaagtgt ttccgtagaa tctcaaaaaa
     9121 ctgagaaaga agaaaaacct gccaaaaaag aagaaataaa aatttctgaa agtgaatcag
     9181 ttagtgcaaa agaagataaa tcaaccagtc cggtagtaag acggatgatg gctgaaaatg
     9241 atttgaaagc cgggcagatt caaggtaccg gtaaagatgg caggataacc aaagaagatg
     9301 ttttatctta catagaatcc aatagagaaa aatcaaacca aactgctgaa gttcaaaaag
     9361 aatcttctat gagagcactt tcttttggtg gcagggaaga aaaacgtgtc cctatgacga
     9421 gactaagagc caagattgcg gaacgtttat tacaagcaca gcacaatgct gccatgttaa
     9481 ccacttttaa cgaagtgaat ttaaaggcag ttatggatat gcgttctcag tataaagata
     9541 gctttgaaaa aaaacatggt gtaaaacttg gatttatgtc tttttttacg aaagcggtag
     9601 tggagtcgct taaacgattt ccggctgtaa atgcttcaat tgatggccaa gatattgttt
     9661 atcatggctt ctatgatata ggaattgctg tttctactga aagaggttta gtcgttccgg
     9721 tgattagaga cgctgatcag ttaagtatgg cagaaattga attagccatt aatgacgctg
     9781 catcaaaagc cagaaccgga aaattatcta tggaagagat gcaagggggg acatttacca
     9841 taactaacgg tggagtcttc ggttcactat tagctactcc cattatcaac ccaccgcaaa
     9901 ctggtatttt agggatgcat aaaattgagg aaagacctat tgtagagaaa gggcaaatag
     9961 taatccgtcc tatgatgtat gttgctttat cctatgatca tcgtttaatt gatggaaaag
    10021 attctgtaca atttttagtg agtgttaaag agctgttgga agatccatcc cgacttttac
    10081 tgaatgttta atgagtcatg tttaactgca agacccgtct tgacgggttt tgctttttta
    10141 aaaggtaatt agaatgaatt tacatgaata tcaagccaag caattgttcg ctagctatgg
    10201 tttaccagtt cctcgtggtg aggtcgctta taatgttgag gatgccttgc tcgttgcttc
    10261 tcaattatca acctctcgat gggttgtgaa agcacaagtc catgctggtg gaagaggtaa
    10321 agcaggtggt gtgaaactgg tttctagcaa agatgaattg gctgcagttg cgaaatccat
    10381 gttaggcaca cgtctggtta cttatcaaac tgatgccaga ggtcaaccag ttaatgctat
    10441 tttggttgaa gaaacctgcg aaatagataa ggaattatat cttggtgctg ttgttgatag
    10501 atccaccaga cgagttgtca ttatggcctc cacagaaggt ggtgttgaaa ttgaaaaggt
    10561 tgctcatgaa acacctgaaa aaattttcaa ggtggttgtt gaccccttag ttggtgttat
    10621 gccctttcaa tgtcgtgaaa cagcttttaa acttggatta aaagacgatc aaatcaaaca
    10681 attcactcat ttgatgatgg gattagggaa aatgtttgtt gattgtgatt taagtttatt
    10741 agagataaat ccgcttgtca taacaaaatc agggcaactg atttgtttgg atggaaaaat
    10801 taatatcgat ggaaatgctt tattccgaca gcctaaattg aaaaatatgc gagacgtttc
    10861 ccaggaagat gatcgagaaa atcgtgccag tgattgggaa cttaattata ttcctttgga
    10921 tgggacaata ggttgcatgg taaatggtgc tggtttggct atggctacca tggacgtgat
    10981 aaaattacat ggtggtgaac cggctaattt cctggatgtt gggggaggcg ccacaaaaga
    11041 aagagtcagc gaagctttga agattattgt gtcagatgaa aaagtcaaag gaattctagt
    11101 taatattttt ggtggaatcg ttcgttgtga ccttattgct gatggaattc ttgctgctgt
    11161 aaaagaagtg gatgtaaaaa ttcctgttgt tgtcagactg gaaggtaata atgcccagtt
    11221 aggcgctgag atattgaata aaagcaattt aaatgtgatt gctgcaacga gtttgacaga
    11281 cgcagccaag aaaattgtag cagcagtttc cgaataaaga ataaattaat tttggcatta
    11341 ttggtaaggt agttttcaaa gcggatatgc cattaaatta cgaggttaac aatgagtgta
    11401 ttagttgata aacatacaaa agtactatgt cagggtttta ctggaaaaca aggtacttat
    11461 catacagagc aagcgctggc ttatgggact aagatggtgg ggggagttac tccaggtaag
    11521 ggtggtcaaa tgcatctggg gttgcctgta tttaatacta tgcgtgaagc agttgaggaa
    11581 acccaggcgg atgcaagcgt tatctatgtt cctgcaccat tttgcaaaga ttccattatt
    11641 gaagcagcag aagctggtgt taaactgatt gtttgtatta cagaaggtgt gcctgtattg
    11701 gatatgttgc aagtaaaagt gtatatagaa aacaatacac aggctcgatt aattggtcca
    11761 aattgtccag gaataattac accaggcgaa tgcaagatag gtattatgcc tggctatatc
    11821 catttacccg gaaaagttgg aatcgtttca cgttcaggaa ctttaaccta cgaagcagta
    11881 aaacaaacta ctgataaagg gtttggtcaa agtacttgca taggaatagg tggcgatcca
    11941 attccaggaa ccagctttat tgatgctttg tctttatttg aaaaagatcc taaaacagaa
    12001 gctatcatca tggtaggaga aattggtggt tcagctgagg aagaggcagc tgaattcatt
    12061 aagtctcatg taaaaaaacc tgtagtatca tacatcgctg gagtcaccgc acctgctgga
    12121 aagcgtatgg ggcatgctgg agcaattatc tctggtggga aggggaccgc agcagaaaaa
    12181 tatgctgcac tggaagcggc tggtgtgaaa acagttcgat ctcctgctga cttgggagac
    12241 gcaatagctg aggtaactgg ttggtaagaa aatacttttt cttacttaac agctcttgca
    12301 ttatagaaat aggaactgat taaaataaat tggtgatttt tcctcgaatc atgttggtgc
    12361 tgtgtatcaa gcgcagccca acagccatca tgataatatt gtcacttggt taaataagat
    12421 tagatccatt tcataacctg ccctaaaaca aaatcaggtc ttaggacccc caatctcgtt
    12481 gttaaaaatt tttttaattt agtttattgc catattccct cattaaa
//
LOCUS       DASLOE010000085        12353 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.086, whole genome shotgun sequence.
ACCESSION   DASLOE010000085 DASLOE010000000
VERSION     DASLOE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12353
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.086"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            81..461
                     /locus_tag="U6309_001822"
     CDS             81..461
                     /locus_tag="U6309_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505261.1"
                     /translation="MFRYFKQGWHGDLKFSEVLFGSGGDYFLLEGGLAYIGFYILFAI
                     LLITSKPLSLDNILALALFTYGIVLYIWLIKAFWGSAHHCSNKVSAVLIRTFTILLPL
                     ISIVLFFLIIIYYLVTAITDALSG"
     gene            515..1642
                     /gene="cfa"
                     /locus_tag="U6309_001823"
     CDS             515..1642
                     /gene="cfa"
                     /locus_tag="U6309_001823"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HEN5505262.1"
                     /translation="MDIKKNKAKTFVNYMLQLANININGQTPWDIQIYNDEFYSRLLR
                     DADLGLGESYMDGWWDCQRIDLFISKLINANLESKIKINFKLAFNVFLSKILNLQTPK
                     RSLHVGRQHYDRGNDLFQIMLDSNMNYTCGYWKKAENLEQAQLDKLDLACRKLCLKPG
                     MKLLDIGCGWGGLAKYAAENYGVSVVGITISQQQYDLAKTRCAHLPVEIRFQDYRDLN
                     EKFDRIVSLGMFEHVGYKNYRKYMEIVHQCLNDDGLFLLHTIGSNESVTKATPWISKY
                     IFPNGMIPSIMQIGQASEKLFVMEDWHNFGADYYKTLMAWHENFNQGWDQIKSQYSEK
                     FFRMWNYYLLSCAGAFDARMLQLWQIVFSKKGIHGGYSAPR"
     gene            1807..2133
                     /locus_tag="U6309_001824"
     CDS             1807..2133
                     /locus_tag="U6309_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505263.1"
                     /translation="MPKNNEPSGEELKKAGIVVGITNLLLWPFYYADSRWGLFASIFA
                     TSAVLYASHEVGESKRPVENSVTRLGLFQQRGASNAEIENAFNNTVTGAAAIYDWLVP
                     GSNGPK"
     gene            2295..3746
                     /locus_tag="U6309_001825"
     CDS             2295..3746
                     /locus_tag="U6309_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEN5505264.1"
                     /translation="MKFRTKLFLGLLLIVITTNGIFLFLSYQYGKETVYKEVGSSALS
                     IAAATAVLMNPEDIQKFNLDTSANSPLYKDLEKKLLEVRNANRRKDVYVNFIYSLFPD
                     SNNPKVLRFAVDPEENIIDKTELGTVMKLRTESGQTINVNYESPIILPEFVEDIWGTW
                     LTALYPIKDAQGKYLGNIRVDVDAGSVNQRFHFLLYSGIAVFISTFLFAMFLGWLLFQ
                     WFNKPLLKITTALKNIAQGDLEQHLDIRTKDEFAEVGRVINEMTEGLRQRNMLHVSLT
                     RFISHALAEKIVKSGELPQVFSERRKVTIMVCDIRDFTTISERIKPELVVDFLNHFFE
                     KMIEAITSQHGILDKYLGDGFLAIFGSPDDDAYQEDHAIHAALKMREAMKSLNADWAK
                     ILGTEIQIGIGINTGSAIVGNIGTDIHMEYTAIGDTVNLASRIENATKKLNTDILISE
                     YTYIATDHTAFQFIELGEISIKGRVHKVKVYTL"
     gene            complement(3910..5472)
                     /locus_tag="U6309_001826"
     CDS             complement(3910..5472)
                     /locus_tag="U6309_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505265.1"
                     /translation="MPTGIILSSQSPEATTSFPRRGGTAAGINHVTGFPVIKMHRDAQ
                     ARPDERIHFNQIKDGSLVFITGHSNTALDHLTGDYVPPLATPVQPMSTDWTYKQFRDL
                     ILKNGNLKAGDTITVVLWACNAGEGGLSSGAGLLGRLFREKQINTRVIASAATTMRFD
                     GHYSPTPDGTPAMAFITENGSKDIRIFDFSEEATIEWKNKGHLYVNNNGIEGYNLYSP
                     KQLESLESEKIYKVMEEIFVDRHYKENIRTGEEVKQYFSKSKTEFILRWSSANENDTE
                     NKYVFIAASFQATDGIHSIRYGINKNGELFSINTTSNKVTPIDILPLGVMATLTQHIT
                     QNKELIEKSLHKKGAQMPPSPRLQHGLKRKKSDHTELLLLKGEKELSLKAVQTDILSS
                     KVPASPRRSIQENQDFLMFVTPLQVQELLKPHGKTMKKKHERKNRLDDGETSSNEKIS
                     RPISKPKKTSGENEEPKMDLSKRQRIPVVQRKRLEGRRHGFFSDSPKNPDEHKTEKDK
                     TTLHSRNPTKGA"
     gene            complement(5900..6364)
                     /locus_tag="U6309_001827"
     CDS             complement(5900..6364)
                     /locus_tag="U6309_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEN5505266.1"
                     /translation="MDFIKRTIELARENVGNGGRPFACLIVQNGKILAEATNTVAQTH
                     DPTAHAEVNAIRIATAKLGCEHLYDCDFYILAHPCPMCLAAMYYCSPRKVIFITTRDE
                     YKTYYVDDRKYFTLENFYQEINKPWRERKMPMEHRPDKEAIEVYKMHQKKKS"
     gene            6447..7955
                     /locus_tag="U6309_001828"
     CDS             6447..7955
                     /locus_tag="U6309_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HEN5505267.1"
                     /translation="MTKKRIDRLIFSEVECNMKKCSMVIAGKSISYEKLFSTVNRIAI
                     HFQNKALSGSRIAFMLPNSLEIIAIYLACFQSGCIAMPVNRRYAPPELEKVLRDAQPI
                     YLIIEASKLFLLEKINWSATGIKKIFVLGENQNHPYFSFNELLHEPANYPKHSIDYKA
                     PAVIFYTSGSTGEPKGVVHTLSSIEAMLDSTSLALENITADDKMIICEPQCHVSGFIE
                     TFSTLSREGTTLVHDGFDMDWYLATVRHEKPTLAVHHIDTYIKLLDSGRCNTKTFQSF
                     RGIYTGGDALPVAVQDKFLACAGKPIQVGYGMTEAIWLTVCRTPDLTQKSCIGKPVDD
                     VSLRLVNAAGEDVHCGETGEIQVKGDMVMHSYWNNPEETRKAFIKGWFKTGDCGIQDE
                     QGYFYYKGRIKNIIIRNTSNIMPGEVEAAIYKHPAISSAAVIGIPDEAEGEVPIAFVV
                     VKKSNQLAREELYSFLLEQIAQYKIPVKIYFIDEMPLTNSGKINHKKLYDFL"
     gene            complement(8049..11480)
                     /locus_tag="U6309_001829"
     CDS             complement(8049..11480)
                     /locus_tag="U6309_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEN5505268.1"
                     /translation="MKILKTTEKKSRKPTPKENRALPLQLVQNTEPDRTYYLRSTVEG
                     IGSFLSTRVRELYNTLAMTFSLDSMDWSSFKKYIWPTISSGAALLLGDYLTRSMSEYQ
                     TGSPEYDFIFKFLFGTAILGYCALNRITDRKFTSQVELSAAVASEVMAITYMYSSVFK
                     GTNYLLNDSISDEYSLIAGLGTSALLVPSGISYLTQKIQEFLVRIQYNKGAQRSDTAV
                     ISSGLTPISNLYFQVNHFIASELMISSRAFQLGLISHNIRYADRIMQKFRNLPALLAD
                     LAPSTIKKMRIQQQKLEHDYEYNHKLHQVLRFTADGSMFISIPRYQLRTGDLVYCDSS
                     IDLACVPVSGELVALRRDLKGEFTQTLEQKKFSVNLKAQNGEDVWIEHETKPDFSSHY
                     KKVDLHAVRDGKQAGVLVGDKLNLYGNDNIFIQIKPEKELLLSSNYEKKAVINEIIAE
                     RKQRSVLYSILGSVIMAAFLQRDITAMPAETLRLMFTLFQTMIPFSEAFLRETVNSRL
                     MKKLNSDLGDQPFGTIDALRVVDLCNALGGYYRDRFPNGVAIISDKTGTLTTTRMDVL
                     GLWTNDMAPEVQKTLKEKEGLLLPEETQWLQTFEVFCNAFTNNKKELEPEEHAILELF
                     QSLLDNRQCLEVITLGNNHFKKEMISKDIKKEIETFHLGLYRTFGGRFTLVQDGSDHY
                     LVFCGVPKQEAFKETPLLKAYTTMQSRTGVLSRDWCLARGKLSEREFTILKELFSKDD
                     KKQIEHFILENKLLLENLMYYGTFIIDNPVKKGAEKFIAQCREISVPVFVATGDTTKA
                     AENIAKVLCPANAGKIVTLRADQIDEKGVEFLTEENYPSDSTVIFSGINEAVLTRFQK
                     LMDREGAKRPVIIFAEMSTEGKGILARYLKDHHYFVVANGDGTNDVMMMKNSNVVIAH
                     HSDDGTFAPGVDGLSNISEEQLRRLFGSQKTFYELFDINLPKSLFVQKFAPLANSQEK
                     PTMALALKSGKMTFDLAKTVGADVTEMNQQHWYSVVFDLLWLWIAFYEINESSDLPMD
                     NRNINASRLISNTMGIALIIAVFQSLANYALFNESTNLTSMLIMLSFLPLILKSIFSG
                     FRMVQDSLYPQVDVTEVEEEGDTNTNRSVLSYFGTFFSRKPVKQLEGLNCVPVLK"
     gene            complement(11665..12333)
                     /locus_tag="U6309_001830"
     CDS             complement(11665..12333)
                     /locus_tag="U6309_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN5505269.1"
                     /translation="MATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENF
                     NDIFLGLANKEYDVVISGTTITPNRSKQVLFSNPYLEFNQGIAVNTELKPNVSSFEDL
                     KGLVAGIQKGNTSDEVARDLLGKNILSDIKYYAYNEIELAIQDLSAGKIGLVIKLYPV
                     ISCLIRNYTNLAVPLQAETHEKLGIAVAKDNFKLRDKVNLILADLMQNGTLSRLQHKW
                     ISTT"
BASE COUNT         3859 a         2359 c         2193 g         3942 t
ORIGIN      
        1 agtaagtcct gaaaaattct ttcatccatt gttctagctt ggcttataat aaaaattata
       61 aataacataa caggatccgt atgtttcgtt actttaaaca gggatggcat ggagatctga
      121 aattttctga agttctgttt ggttcaggtg gtgattattt tttgcttgaa ggcggcctgg
      181 cgtatattgg attctatatt ttatttgcca ttttgttaat aacatctaaa ccattatccc
      241 tagataatat attagcccta gctctcttca cctatggcat agtcttatac atctggttaa
      301 ttaaagcatt ttggggatcg gcgcatcatt gctcaaataa agtttcagca gttttgattc
      361 gtacctttac cattcttttg cccttgattt cgattgtgtt gttttttctg attattattt
      421 attatcttgt tacagccata acagacgctt tgagtggctg atgatgttgc attgattgaa
      481 tgtttcagca tcctttataa aaacggagtt tgatatggat attaaaaaaa acaaagcaaa
      541 aacatttgtc aattacatgc ttcagctagc caatatcaat atcaatggcc aaacaccttg
      601 ggatatacaa atttataatg atgagttcta ttccagatta ttgcgtgatg ccgatttagg
      661 tttgggtgaa agttacatgg acggttggtg ggattgtcaa cgaattgatt tatttatttc
      721 aaaactgatt aacgcaaatc ttgagtccaa aattaaaatt aattttaaat tggcatttaa
      781 tgtcttttta tcaaaaatat tgaacctgca aaccccaaaa cgttctctac atgttggtag
      841 acaacattat gatagaggaa atgatctgtt tcagattatg cttgatagca acatgaacta
      901 cacttgtggt tattggaaaa aggctgaaaa tttggagcaa gcccaactcg acaagctcga
      961 tctggcctgc agaaaactct gccttaaacc cggtatgaaa ttgctggata tcggatgtgg
     1021 atggggagga ctggcaaaat atgctgctga aaactatggg gtatcagtag ttggcataac
     1081 gatatcacag caacaatacg acttagctaa aacacgctgt gctcatttgc cggttgaaat
     1141 ccgatttcag gattatagag acttaaatga aaaattcgat cgtatagtct cccttgggat
     1201 gtttgagcat gttggttaca agaattaccg aaagtacatg gagattgtcc atcaatgctt
     1261 aaatgatgat ggtttgttct tattacacac tataggttcc aatgaaagtg tcacaaaagc
     1321 cacaccctgg atttcaaaat atatttttcc caatggaatg attccatcca tcatgcaaat
     1381 aggccaagcc tctgaaaaat tattcgttat ggaggattgg cataattttg gagctgatta
     1441 ttacaaaaca ttaatggcat ggcatgagaa tttcaatcag ggatgggacc aaattaaatc
     1501 ccaatatagc gaaaaattct ttcgtatgtg gaattattat ttgctctcgt gtgctggagc
     1561 ttttgatgcc agaatgttgc aattatggca gattgttttt tcaaagaaag gaattcacgg
     1621 aggttactct gcgccgaggt aaatggttta aacgatttat tccgcatcgt aataccttca
     1681 cgagcaaacc tggttttcca tgccatgaag ttggcaagat tattcatata tcccattaat
     1741 tttgcttgca atttttatta tctggaatat agtttgaact gaaattttgt ctaaaaagga
     1801 attaatatgc caaaaaataa tgaaccaagt ggagaagagc tcaaaaaagc agggatagtg
     1861 gtaggaataa caaacctgct tttatggcca ttttattatg ccgactctag atgggggttg
     1921 tttgcatcca tattcgctac ttccgcagta ctttatgctt ctcacgaagt cggagaaagt
     1981 aaacggcccg ttgaaaactc agtgacgcga cttggtctct tccagcagcg tggagccagt
     2041 aatgctgaaa tagaaaatgc ttttaataat acagtcacgg gtgccgctgc gatatatgat
     2101 tggcttgttc ctggctccaa tggccccaaa taataagtat cctgtcttat aataaattat
     2161 atttttggga agtattattt tatccaaaac cacatcgttc tggagattgg taaagatctc
     2221 cagaaatctg ttgtctttac ttctcactgc ctttatccaa tacttataat taatctgcta
     2281 atggtggata aataatgaaa tttcgtacta agttatttct tggcctactt ctcattgtaa
     2341 tcactactaa cggcatattt ctgtttttat cttatcaata tggtaaagaa accgtataca
     2401 aagaagtagg ctcatcagcc ctatccatag ctgcggcaac agccgtcctg atgaatccgg
     2461 aggatattca gaaatttaat ctggatacgt ctgcaaactc acctctttac aaagatcttg
     2521 aaaaaaaact tcttgaagta cgtaacgcaa atcgtcgcaa agatgtgtat gtgaatttca
     2581 tatatagttt atttcccgac tctaataatc ccaaggtact tcgctttgcc gtagatcccg
     2641 aagaaaacat tatagataaa actgaattgg gaactgtcat gaaacttagg actgaatcag
     2701 ggcagacaat taatgtaaat tatgaatcgc ctatcattct gcctgaattt gttgaagaca
     2761 tctggggtac atggctgacc gctttatacc caataaaaga cgcacaaggg aaataccttg
     2821 gaaatatccg ggtggacgta gacgccggtt cggttaacca aagatttcat ttcttgctgt
     2881 actcaggtat tgccgtcttt atctcaacat ttttatttgc catgttttta ggttggttac
     2941 tatttcagtg gtttaataaa cccttattaa aaattacaac tgcactcaaa aatattgccc
     3001 aaggtgatct tgaacagcat ctcgacatta ggacaaaaga tgaattcgcg gaagtgggtc
     3061 gagtaatcaa tgaaatgact gaaggattga ggcagcgtaa catgctgcat gtaagcttaa
     3121 cacgttttat ttctcatgct ttagcagaaa aaattgtgaa atccggtgag ttaccgcaag
     3181 tatttagcga gagacgtaag gtcacaatta tggtctgtga tatacgcgat ttcacaacca
     3241 tttcggagcg tattaaacct gaattggtag ttgatttttt aaatcatttt tttgagaaaa
     3301 tgatagaagc tattacctcg caacatggaa tattggataa atatcttgga gatggttttt
     3361 tagcgatttt cggttcacct gatgatgacg cctatcaaga agaccatgct atccatgccg
     3421 cattaaaaat gcgagaagcg atgaaatcac taaatgccga ttgggcaaaa atattaggca
     3481 ctgaaataca aattggtatt ggcatcaata caggcagtgc cattgtcggg aatattggaa
     3541 ctgatataca tatggaatac acagctattg gcgatactgt caatttggcg tcacgaatag
     3601 aaaatgccac caaaaaactt aatacagata ttctaatcag tgaatatact tatattgcga
     3661 cagatcatac ggcatttcaa tttatagagt taggagaaat ttctatcaaa ggaagagttc
     3721 ataaagtaaa agtttatact ctttaaaatt aattgtccgc tgacttgcat aatcaccagc
     3781 tcctttaaag gcaaaaagca cccacagctg cctatttaag atagaaagta tgtataacat
     3841 atcagctact aacttgtggt ttgatgtaac gtagttttga tgaacatcca acaagactat
     3901 actgaccagc taagcccctt tggttggatt tcgcgagtgc aatgtcgttt tatccttttc
     3961 tgttttatgc tcatctggat tttttgggga atcactaaag aacccgtgcc tacgaccctc
     4021 aagtcgtttt ctctgtacaa caggtattcg ttgcctttta ctcaaatcca tttttggctc
     4081 ttcattttct ccagaagttt tttttggttt agatataggc ctgcttattt tctcattact
     4141 ggaagtttct ccatcatcca atctgttttt tctctcatgt tttttcttca tcgtttttcc
     4201 atgaggttta agcaactcct gtacttgcaa cggtgtaaca aacattaaaa aatcctgatt
     4261 ttcttggatt gacctccttg gagaagcagg caccttgctt gagagaatat ctgtttgaac
     4321 agcttttaat gacaactctt tctcgccttt taaaagaagc aattcagtat gatctgattt
     4381 tttacgtttc aatccatgtt gtagtcttgg actggggggc atttgagccc cttttttatg
     4441 gagcgatttc tctatcaatt ctttattttg agtaatatgc tgcgtcaaag tagccattac
     4501 acccagtggt aaaatatcaa ttggcgtaac tttattgcta gtcgtattta ttgaaaaaag
     4561 ttctccattt ttgtttattc catatcgaat gctatggata ccatcagtgg cctgaaaaga
     4621 tgctgcaatg aagacatatt tattttcagt atcattctca ttggctgaac tccaacgcaa
     4681 tataaactcc gtcttagact tggaaaaata ttgtttcact tcttctccag tcctgatatt
     4741 ttccttgtag tgcctatcaa caaatatttc ctccatgact ttatatattt tttcactttc
     4801 cagggattct aattgtttag gactgtacaa gttatagcct tcaatcccat tgttgttaac
     4861 ataaagatgc cctttatttt tccattcaat cgttgcttcc tcagaaaaat caaaaatgcg
     4921 gatatctttg ctaccattct ctgttataaa tgccatagct ggcgttccat ctggagttgg
     4981 agaatagtga ccatcaaatc gcatagtagt tgctgctgaa gcaataactc ttgtattaat
     5041 ttgtttttcc ctgaataagc gccccaataa gcctgcccca ctactcagac ctccctcacc
     5101 tgcattacat gcccaaagaa cgacggttat cgtatcacct gccttgaggt ttccattctt
     5161 cagaatcaga tctctaaact gtttatatgt ccagtcggta ctcattggct gaaccggagt
     5221 cgctaatgga ggaacataat cgccagtaag atgatccaaa gcagtattgc tatgtccggt
     5281 aatgaaaacc aagctgccat ctttaatttg attaaagtgt attcgctcgt caggacgagc
     5341 ctgagcgtct ctatgcattt taataacagg aaaaccagtc acatggttta ttccagcagc
     5401 agtacctcct cgacgaggaa aagaagttgt ggcttcaggg gattgtgaac ttaatatgat
     5461 tccagtaggc attagtcata ctcaattagt ttatcttaaa aatttgagca aaattatatg
     5521 caattagatt tttattggaa cttcaaagtt gtttttcttt gctcaattgc gagtgcttta
     5581 taatgatcaa cgggaatcat cttgattttg aaggaaactt tccattaact tcgtgctgtc
     5641 taatataaaa ccagcatagt ttattataaa aacattaaga ttatattaat aacaatcagc
     5701 attgaaataa aaaatacatt tgagatgttt taaaattttt aaggtatgct caaaaggtag
     5761 actgaaaaaa gcaggaagat actacccaag ataaacatga tttattgaaa gtgatcaagc
     5821 aagcctatga actttaaaac ataaaagttt tttaaaggtt gaaaatgaaa ctgttttaaa
     5881 tattagacag ttatttggac taagactttt ttttctggtg cattttatat acttctattg
     5941 cctctttgtc cggtctatgc tccataggca tcttacgttc tctccatggc ttattgatct
     6001 cctgataaaa attctcaaga gtgaagtatt ttcgatcatc aacataatag gttttatatt
     6061 catctctcgt tgtgataaag atcactttcc tgggactaca ataatacatt gcggccaagc
     6121 acatcgggca aggatgagca agtatataaa aatcgcaatc gtataaatgc tcacaaccta
     6181 atttcgctgt agcaatccgt attgcattaa cttcagcatg ggctgtagga tcatgggttt
     6241 gcgccactgt attggtagct tcagcaagaa tttttccatt ctgaactata agacaagcaa
     6301 atggtctgcc cccatttccc acattctctc gagccagctc gatagtacgc tttataaaat
     6361 ccattttttt ctcattttta gcccataatt attaattatt tcccataata taaaaagtaa
     6421 caaatgaatc tatccgggaa aacataatga ctaaaaaaag aatagacagg cttatttttt
     6481 cagaagttga atgcaatatg aaaaaatgct ctatggttat tgcaggcaaa tccatatcct
     6541 atgagaaatt gtttagtaca gtgaatcgta ttgcaattca ctttcaaaat aaagcactct
     6601 ctggaagccg tatcgctttt atgttgccaa atagtcttga aattatagcc atttacctgg
     6661 cctgttttca atcaggatgt attgccatgc ctgttaatcg ccgctatgcg ccacctgagc
     6721 ttgaaaaggt attgcgcgat gcccaaccaa tttatttaat tatcgaagca agtaaattat
     6781 ttttgctaga gaaaatcaat tggtcagcga caggaattaa aaaaattttt gtcctggggg
     6841 aaaatcagaa tcatccctat ttttctttta atgaattatt acatgaacca gcgaattacc
     6901 caaaacatag catagattat aaagcgcctg ccgtaatttt ttatacctcc ggctcaacgg
     6961 gagaacccaa aggagttgtt catacgcttt cttccattga agcgatgctt gactcgactt
     7021 cattggcact ggaaaacatt accgctgacg acaaaatgat aatctgtgag ccacaatgcc
     7081 atgtaagtgg atttatcgaa acattctcta cattatcacg agaaggtacc acgttagttc
     7141 atgatggttt tgatatggat tggtatcttg cgacagtacg acatgaaaaa ccaacactcg
     7201 ccgtacatca tattgatacc tatataaagt tattagactc tggacgctgt aatacaaaaa
     7261 catttcaatc atttcgtgga atttatacag gtggggatgc actacccgtg gcagtccagg
     7321 acaaatttct ggcttgcgct ggtaaaccaa ttcaagtggg ttatggaatg acggaagcca
     7381 tttggctaac agtctgcagg acccctgatt tgactcaaaa atcctgtatt ggcaaaccgg
     7441 tggatgatgt gtcattacgc cttgtaaacg cagctggaga agacgtacat tgtggtgaaa
     7501 caggagaaat acaggtaaaa ggtgatatgg tgatgcatag ttattggaat aatcctgaag
     7561 aaacccgcaa agcatttata aaaggttggt ttaaaacagg agattgcgga attcaggatg
     7621 aacaaggtta tttttattat aaaggtcgga ttaaaaacat tattatccgc aatacatcca
     7681 atattatgcc tggtgaagta gaagcagcca tttataaaca tccagcaatt agctcagcgg
     7741 ctgtgattgg tatacctgac gaagcagaag gagaagtacc cattgctttt gtagtcgtga
     7801 aaaagagtaa ccaattagcc agagaagaat tatattcttt tttacttgag caaatcgctc
     7861 aatataaaat acctgtaaaa atttacttta ttgatgagat gcccttgact aatagtggaa
     7921 aaattaatca taaaaaattg tatgattttc tttaaaatta aaaatgccta gcttgtatac
     7981 agtcaatgag tattacagaa acatttacac atagcggcaa aacaaaaaac cccggttatg
     8041 caaaggggct actttaatac agggacacag tttaaaccct ccaattgctt tactggcttg
     8101 cgtgaaaaaa aagttccaaa atagcttagg acagatctat tagtatttgt atctccttct
     8161 tcctcaactt cggttacatc cacctgcggg tacaagctgt cctgaaccat tctaaaacct
     8221 gagaaaatac tctttagtat tagaggcaaa aaactcaaca taataagcat actggtcaag
     8281 ttcgtagatt cattaaacag agcataattg gcaagggatt gaaaaacagc gattatcaag
     8341 gcaatcccca tggtattgga aatcaatctt gaagcattga tgttccgatt atccattggc
     8401 aaatcggagc tttcatttat ttcataaaaa gcaatccaca accataataa gtcaaaaaca
     8461 acgctatacc aatgctgctg attcatttct gtgacatctg ctccaactgt tttagccaaa
     8521 tcaaacgtca tttttccact tttgagtgcc aaggccatgg ttggcttttc ctgtgagttg
     8581 gctagaggag caaatttttg aacgaacagg cttttgggta gattaatgtc aaacagttca
     8641 taaaaagttt tctgggaacc aaacaaccgt cttagctgct cctcactaat attggataag
     8701 ccatccactc ctggagcaaa agttccatca tctgaatgat gcgctatcac cacattggaa
     8761 tttttcatca tcataacatc attggtacca tccccgttgg caacaacaaa atagtgatgg
     8821 tccttaagat aacgagctaa aatacctttt ccctcagtac tcatttcagc aaaaataatg
     8881 actggtcgtt ttgccccttc tctatccatc agtttttgaa atctggttaa taccgcttca
     8941 ttaatacctg aaaatataac tgtagagtca gagggataat tttcttcagt taaaaattca
     9001 acaccttttt cgtcaatctg atcagcacgc agcgtaacaa tttttcctgc attagccggg
     9061 cacaatactt tagcaatatt ttctgctgcc ttggtcgtat ctcctgttgc tacaaaaact
     9121 ggaactgata tttcacgaca ttgggcaata aatttttctg ctcctttttt tacaggatta
     9181 tcaataataa aagtaccgta atacatcaaa ttttcgagca ataatttatt ttccaaaata
     9241 aaatgctcaa tctgcttttt atcatccttg ctaaataatt ctttcaaaat agtaaactcg
     9301 cgttcactga gtttacctcg agccaaacac caatcacgag ataacactcc agtacgactt
     9361 tgcatagtcg tataggcttt tagcaaagga gtctccttaa aagcctcttg tttgggcacc
     9421 ccacaaaaga ccagataatg atcagaacca tcctgaacca aagtaaagcg cccgccaaac
     9481 gtacgatata aacccaaatg aaaagtttca atttcttttt taatgtcttt agagatcatt
     9541 tcttttttaa aatgattatt ccctaaagta atcacttcca gacactgccg attatcgagt
     9601 aaggattgaa ataactctaa aatagcatgt tcctcaggtt ccaattcttt tttattgtta
     9661 gtaaacgcat tgcaaaaaac ttcaaaagtt tgcagccatt gtgtttcttc tggcaagagc
     9721 aaaccctctt tttcctttaa tgtcttctga acctcaggag ccatatcatt agtccataac
     9781 cctaatacat ccattcttgt agttgtaaga gttccggttt tatctgaaat aatggcaaca
     9841 ccattgggaa atctgtcacg ataataacca ccaagagcat tacataaatc aaccacacga
     9901 agagcatcta tagtcccaaa aggctgatcc ccaagatcgc tattcaattt tttcatcaaa
     9961 cggctattca cagtttctcg taaaaaagct tcagaaaaag gaatcatagt ctgaaagagt
    10021 gtgaacatga gccttagggt ttctgcaggc attgcagtaa tatccctttg taaaaaagca
    10081 gccatgataa cggagcctaa aatagaatat aaaacacttc tttgttttcg ttcagcaata
    10141 atctcattaa tgactgcttt tttttcatag ttactgctta ataaaagctc tttctcgggt
    10201 ttgatttgaa taaaaatatt atcattacca tataaattta atttatcgcc taccaatact
    10261 cctgcctgtt tgccatctct aacggcgtgc aaatccacct ttttataatg cgagctaaaa
    10321 tcaggcttgg tttcatgttc tatccaaaca tcttccccat tttgggcttt caggttaacg
    10381 ctaaattttt tttgttccag ggtttgagta aactctcctt ttaaatcacg ccttaaagca
    10441 accagctccc cagaaaccgg tacacaagcc aaatcgatgc tgctatcaca ataaaccaga
    10501 tcccctgtgc gtaattgata ccttgggata cttataaaca ttgagccatc tgctgtaaat
    10561 cttaaaacct gatgtagctt atgattgtat tcgtaatcat gctcaagctt ttgttgctgg
    10621 atacgcattt tttttatcgt cgatggtgcc aaatccgcta ataatgcagg caagttacga
    10681 aatttctgca ttattctatc cgcatagcga atattgtgtg aaatgagccc cagctgaaat
    10741 gcgcgtgaac tgatcatcag ttcgctggca ataaaatgat tgacttgaaa ataaagattg
    10801 gatatgggtg tgagcccgct tgaaatcact gctgtatccg agcgttgcgc acccttattg
    10861 tattggattc gcactaaaaa ctcttgtatt ttttgtgtta gataagaaat tccgctagga
    10921 accagcagcg cgctggttcc tagcccggca attaatgaat attcatcaga aatactatca
    10981 ttcaataaat aattggtgcc tttaaaaacc gaactataca tgtaggttat tgccatgact
    11041 tcactggcta ctgcagcaga taattcaacc tgggaagtaa atttcctgtc agttatccga
    11101 tttaaagcac agtaccctaa tatcgctgta ccaaaaagaa atttaaaaat aaaatcatat
    11161 tccgggcttc ccgtttgata ttcgctcatg gatcgggtta gataatcacc aagcagtaat
    11221 gctgctccag aggatatcgt tggccaaatg tattttttga aactgctcca atccatagag
    11281 tctaaagaaa aagtcatggc taaagtgtta taaagttccc ttacacgggt agaaagaaaa
    11341 ctccctatgc cttctacagt acttctcaga taatacgttc gatccggctc agtattttgt
    11401 accaattgaa gcggtaaagc tctgttctct ttaggagtag gttttcgcga ttttttttcg
    11461 gtcgtcttta atatcttcat gttataaatc ctttcgtact ccaataacac tctattggat
    11521 ctttagagct caacctgata attaacttat ctgtataaaa gttcaagagg atttgaaaaa
    11581 taattacaga acgattcaag aaaaaataag attccttaaa taaggttatt tcttgagttg
    11641 aaactgacat tggcctaatt taccctaggt tgtacttatc cacttatgtt gcagacgact
    11701 taaagtacca ttttgcataa gatcggccaa aataagattg actttatccc gtagtttaaa
    11761 attatcttta gcaaccgcga taccaagttt ttcatgtgtt tcagcctgca aaggaacagc
    11821 caggtttgta tagtttcgaa ttaaacagga aataacagga tataatttaa tgaccaaacc
    11881 aattttaccg gcagacagat cctgtatggc taattctatt tcattgtatg cataatattt
    11941 aatatcactg agtatgttct tacctaataa atcccgagca acttcatctg aggtgttgcc
    12001 tttctgtata ccagccacca aacctttcaa atcttcaaaa gaagaaacat ttggctttaa
    12061 ttcagtatta accgcgatac cctgattaaa ctctaaatag ggattcgaaa aaagtacttg
    12121 ttttgatcgg tttggtgtaa tagtcgttcc tgaaatgaca acatcgtatt ctttattagc
    12181 caagcctaaa aaaatatcat taaagttttc tccctcatat ttgatctgga ctagctgcaa
    12241 atttaaaatt cgacaaattt cttttattaa atcaccatca aaaccatcac ctggctcaat
    12301 atcaaacggt ggatctggat aagcagtagc caccaataat tttcccggct tta
//
LOCUS       DASLOE010000086        12153 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.087, whole genome shotgun sequence.
ACCESSION   DASLOE010000086 DASLOE010000000
VERSION     DASLOE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.087"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1716)
                     /locus_tag="U6309_001831"
     CDS             complement(<1..1716)
                     /locus_tag="U6309_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN5505270.1"
                     /translation="MSGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHN
                     EKTPSFNVVAKKQFYHCFGCGASGNAISFVMNYLNQGFTDAVETLATRLGLSVPRDGT
                     GEKHKSSLNLYNLMSEVSQYYQKKLKYNGQVAIDYLRNRGLSGEIAKRYHLGYAPEGW
                     HNLEKAFPNNQRELLSTGMLIKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRVLDKD
                     QKPKYLNSPETVLFQKSRELYGLHQVLSQQKNPDSIIVVEGYMDVIALAQHGIFNVVA
                     TLGTATSTFHIQLLAKHTKHLIFCFDGDAAGKQAAWRALESSLPHLNAGLDAGFIFLP
                     NEHDPDSLVRNEGKNGFLELLQQATPLHRFFFDTLSKDINLSNPAGKTQLINAVKPYL
                     QKMVEGSYKQLLIDDLARLTHIESHRLTNLITDKSESKPEVQAAISRSPLRIAIALLL
                     QNPEIYSIAIQQINPALLHEQEHHILLKLLQQLKDKPNANTATLIEFWRNSSYFELIV
                     KLAAWDHQVPEQELTKEFIDVLLFLQKQNREILIRQYIEKSRKTGLTEAERLSLQNLL
                     KERHGQ"
     gene            complement(1725..2168)
                     /locus_tag="U6309_001832"
     CDS             complement(1725..2168)
                     /locus_tag="U6309_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2359 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505271.1"
                     /translation="MTIKEQINNDIKDAMRAKDKNLLNALRLISAAVKQIEVDERIEV
                     DDERMLVILDKMSKQRKESIAQFEKASRDDLVAQEQFELNVISKYLPEPLNDNEIEEL
                     ISDAIKSTGAEKMSDMGKVMAILKPKLQGRADMAQVSARIKAKLS"
     gene            complement(2371..2610)
                     /gene="rpsU"
                     /locus_tag="U6309_001833"
     CDS             complement(2371..2610)
                     /gene="rpsU"
                     /locus_tag="U6309_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEN5505272.1"
                     /translation="MPTVRVKEGENPEYALRRFKRSCEKAGILTELRRREFYEKPTAE
                     RKRKQAAAVKRHLKKISRDVSSRRGVGHRRKKSTT"
     gene            2815..3816
                     /gene="tsaD"
                     /locus_tag="U6309_001834"
     CDS             2815..3816
                     /gene="tsaD"
                     /locus_tag="U6309_001834"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEN5505273.1"
                     /translation="MLVLGIESSCDETGIAVYDSESGLLSHALHSQIATHRVHGGVVP
                     ELASRDHVNYLVPLVDEVLTKAQIRKNQLDGIAYTAGPGLIGALLVGSCFAKSLAYAL
                     NIPALAIHHLEAHLLAAKMETPSLDFPFIALLVSGGHCQLIEVNNIGEYRLLGDTLDD
                     AVGEAFDKTAKLMGIPYPGGAVLANLADQCLSTPYQFPRPMTDRPGLDFSFSGLKTHA
                     LNTWNQSEKKESDRSEIAKAFQQAVVETLIIKCKRAIKESQSKRLVVAGGVGANKALR
                     SALQKWIKDIKGEVYFPALEYCTDNGAMVAYAGCLRMVRGESDGGLGVMVKPRWPLA"
     gene            complement(3993..4826)
                     /gene="plsY"
                     /locus_tag="U6309_001835"
     CDS             complement(3993..4826)
                     /gene="plsY"
                     /locus_tag="U6309_001835"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEN5505274.1"
                     /translation="MALFIFLILVGYLMGSINSAIIVCRTFGLPDPREEGSKNPGATN
                     VLRLGGKQYGIMVMVFDALKGILPVILAKFLSAEPVTVAFTALAAVVGHMYPVFFHFR
                     GGKGVATTIGALLAFHFVIGVMVAATWLLVANFWRYSSLASIASISLAPFYSLILVGN
                     LNIFPPLFMITILVLYKHRDNFNRLIDGKEPKIKFKHSVIEEIMEASPATSAEQEFPG
                     KEVIDTNIDETEKTEQAKAVKKPKAKKATTKAKKTTSKEETTKKPKSTKPKTKTVKEK
                     E"
     gene            5180..6589
                     /locus_tag="U6309_001836"
     CDS             5180..6589
                     /locus_tag="U6309_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEN5505275.1"
                     /translation="MDGIHLLSAIEIIRRIKMKELSAAEVMVAHLNHIDKINPVINAL
                     TERIPPEECLKQAKEIDKSIASKKNLNKLMGLPVAIKDALYVKGLICSSACSGFYKGE
                     KAVRDATLVSRLKKEGAIILGLTNVPELCRGGDSDNLIYGRTNNPYDLARTSGGSSGG
                     SAALIAAGGVPFALGSDGGGSLMQPAHCCGIVALKPTHGHLPHTGSVGGDSYGLIGNL
                     ISFGPMARSVSDLRLGLSVLAGSDQYDPYTNPVPVMPAAPLKKLRVAYFTENGFTPVD
                     AEIQNVVKSAALALQDDVAIVREVRPDCVSKAFDLHWELFLGGDRGAGFKTMLSELGV
                     NNLSWELQEFLRQAEQTQFSVTQLHQRMREIDLFRLELALFMQDYDVLISPVFPTVAK
                     PHGIGIKEISDFSYAMTHNLSGFPTISLRCGTSAEGLPINILIAANRWKDTTSLAVAE
                     RLEQLMGGYKPPSLIHSLQ"
     gene            complement(6621..7748)
                     /gene="hemW"
                     /locus_tag="U6309_001837"
     CDS             complement(6621..7748)
                     /gene="hemW"
                     /locus_tag="U6309_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEN5505276.1"
                     /translation="MLPLSLYIHIPWCIRKCPYCDFNSHKSPDVLPEHQYIQALIEDL
                     KTDISSYNTREINSIFIGGGTPSLFSAEAYNNLFNELKRILPFAKNIEITMEANPGTV
                     EQHRFTDYRQSGINRLSLGIQSFNPNHLKILGRIHDEKQAHSAIDTARKAGFDNLNLD
                     IMHSLPNQSVTQGLQDLKTALSYQPEHLSWYQLTIEPNTVFYKHTPPLPSEEEDYLLE
                     EQGFTLLHNSGYHRYEISAFSKPEKQARHNINYWLFGDYLGIGAGAHGKMTTPNEIIR
                     TRKHRQPKDYLDNEKPYLAARETITGKELLFEFMLNTTRLEQSIPLELFTTATGLELN
                     QLLPLLKLANKKQLINLTDTHWQVTPLGRQYTNDLQALFLP"
     gene            7830..8390
                     /gene="nudE"
                     /locus_tag="U6309_001838"
     CDS             7830..8390
                     /gene="nudE"
                     /locus_tag="U6309_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HEN5505277.1"
                     /translation="MRNKPKCKQRTVIAKSRLFTVEEMYLEFSNGAQRVYERIKSHGH
                     GAVLIVALTPGESLLLVREYAAGTDSYELSFPKGVIDRSETPIEAANRELREETGFAA
                     RELHWIRSMSLAPGYFGARIELIVAEGLYHSPLPGDEPEPPETVEWPLHAAYELLEKP
                     DFTEARSIAALFLVKEWLNKRKSYDK"
     gene            8380..9372
                     /locus_tag="U6309_001839"
     CDS             8380..9372
                     /locus_tag="U6309_001839"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEN5505278.1"
                     /translation="MTNNRVRVAVTGAAGQIGYALVFRIASGQMFGPNTEVELNLLEL
                     EPALPSLEGVAMELDDCAFPLLKRIVCTADLNKAMDGVNWALLVGSVPRKQGMERSDL
                     LQINGGIFTKQGQAINDYASDDVRVFVVGNPCNTNCLIAMNHAKDVPSDRFYAMTTLD
                     ELRARTQLAKKAGVDITAVTQMTIWGNHSATQYPDFYNAKINGTSAAQVINDETWLKE
                     TFVSTVQQRGAAVIKARGSSSAASAANAIITGVNHLVTDTPAGESFSMCRRSKGEYGV
                     DEGLIFSFPCRREHGELKVVENLEFNDFGRERFNTTLNELRSERDTVKSLGLLD"
     gene            complement(9470..10390)
                     /gene="mavF"
                     /locus_tag="U6309_001840"
     CDS             complement(9470..10390)
                     /gene="mavF"
                     /locus_tag="U6309_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavF"
                     /protein_id="HEN5505279.1"
                     /translation="MQNIWLLCFYKLLLNKGLWRNIGMEDPFSVLLKSLQSVFHLEAM
                     RNGKYTFPAVQHLKEATENYNPKARNTFIELLRAIREALPYIEKWRINFNVIRKSMDA
                     LAKLHHMPTIDWNQVLSHPKVSPRFQFSALNHSHHEHDLMTWLEKKVGKPFSQLDHTE
                     LTQTLVDNRDRLGFSQKLSNHLKKDPDFLYNIILRSERNFIKISQTRLILYLTDEQLA
                     RAIIKHVPVLMHKRKEPFEQIEQLIHTLNEILSNGRSVSTLLRNTDAKTILENSPLFQ
                     MYLSEEYKNRHQHPNKDPDLQTKESLKPGL"
     gene            10521..11060
                     /locus_tag="U6309_001841"
     CDS             10521..11060
                     /locus_tag="U6309_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEN5505280.1"
                     /translation="MITVGNKFPEFQLKATVANDINNAFQMISNETYRGKWLVVFFWP
                     KDFTFVCPTEIAEFGRLNSEFADRDAQILGGSTDSEFVHLAWRNQHPDLKDLPFPMLA
                     DVKRELTANLGILDDEEGVAQRATFIVDPQGITRFVMVTDLNVGRNPAEVLRVLDALQ
                     TDELCPCNWKKGEETIHIK"
     gene            11083..11598
                     /locus_tag="U6309_001842"
     CDS             11083..11598
                     /locus_tag="U6309_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN5505281.1"
                     /translation="MLENVKESIPEVAKDIRLNLTKVLDLEQSDGLSEHQILGSALAV
                     SYSLGNEVLIKGIKELVHNEQIVRAAQLAASLMAMTNIYYRFVHLTEIKEIEHIPAGL
                     RMQGMMTPGVDRVTFEILSLAVSALNGCGACVSSHARQLKEHGLSDTSLARVGRITAV
                     IHAVHVSGRIK"
     gene            complement(11642..12130)
                     /gene="sodC"
                     /locus_tag="U6309_001843"
     CDS             complement(11642..12130)
                     /gene="sodC"
                     /locus_tag="U6309_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodC"
                     /protein_id="HEN5505282.1"
                     /translation="MNKSGIILIGTILFSSMAIADDLTAPIYTTGPKPVAIGKVTFTQ
                     TPYGVLITPDLTNLPEGPHGFHLHKNADCGNHGMHAEGHYDPQNTNSHQGPYGNGHLG
                     DLPVLYVTSNGKAMIPTLAPRLKLSDMHNLAVMIHANGDTYSDNPPQGGGGDRIACGV
                     IK"
BASE COUNT         3418 a         2257 c         2653 g         3825 t
ORIGIN      
        1 ttgcccatgt ctttccttta ataaattttg taaactgagc cgttcagctt ctgttaagcc
       61 agtttttctg gatttttcta tgtattgtcg tatcaatatc tcacgatttt gtttttgtaa
      121 aaataataag acatcaataa attctttagt cagctcctgc tcaggcactt gatgatccca
      181 ggccgccaat ttaactatta attcaaaata actgctattg cgccagaatt caatgagggt
      241 ggccgtattg gcattgggct tgtcttttaa ttgttgcaat aatttcaata aaatatgatg
      301 ctcttgttca tggagcagag caggattgat ttgttgtatt gctattgaat aaatttcggg
      361 gttttgtaag aggagcgcga tagcaatacg cagaggtgaa cgagaaatag cagcttgtac
      421 ttccggcttg ctttccgatt tatcagtaat caaattagtt aaccgatggc tttcaatatg
      481 agttaagcgt gccaggtcat caataagcaa ctgtttgtat gagccctcaa ccattttttg
      541 caaatagggt ttcactgcat tgataagctg tgtttttcct gcagggtttg acaaattaat
      601 gtctttactt aaagtatcaa agaaaaagcg gtggagtggt gttgcttgtt gcaagagctc
      661 aaggaaaccg ttttttcctt catttcttac caggctatcc gggtcatgtt cattcggcaa
      721 gaaaataaat ccggcatcta atccggcatt caaatgcggt aaactgctct ccaatgctcg
      781 ccaggctgct tgtttacctg ccgcatcacc atcaaagcaa aaaatgagat gtttggtatg
      841 ctttgccaat agttgtatat ggaatgtgct ggtggcagtt cccaacgttg ccaccacatt
      901 aaaaatgcca tgttgcgcca aggcaatgac atccatgtaa ccttcaacaa caataatact
      961 gtcgggattt ttctgctgag acaggacttg atgtaagcca tacaactcac gacttttttg
     1021 aaaaagtact gtttcaggcg agttaaggta tttgggtttt tgatctttat cgagtacacg
     1081 tccgccaaaa ccaatcacac gaccgtttct gtcatggatg gggaacataa ttcgattacg
     1141 atatctgtca taaattttac cctcatcgct tttaatgagc ataccggtac tcaatagttc
     1201 acgctgattg tttgggaaag ctttttccag gttgtgccaa ccttcaggtg catagcctaa
     1261 atgatatcgt ttggcaattt ccccactaag acctcggttg cgaaggtagt caattgccac
     1321 ttgtccatta tattttaatt ttttttggta atattggctg acttctgaca tgagattata
     1381 gagattgaga gatgatttat gtttctctcc tgtaccatcc cttgggacac ttaaacccaa
     1441 gcgcgtcgcg agggtttcaa cggcatccgt aaaaccttga ttcagataat tcattacaaa
     1501 actgatggcg ttacctgaag caccacaacc aaaacaatga taaaattgtt tttttgcaac
     1561 aacattgaat gatggtgttt tctcattatg gaaagggcaa cacgcaatat gactgttgcc
     1621 tctttttttc aaaggaacat aaccgtctat taattccacc aaatcagtgc ggtttaatag
     1681 atcatcaatg aatggctgtg ggattaagcc agacatattg gaactcagct taatttcgcc
     1741 tttatccggg cgctgacttg ggccatgtct gcgcgtcctt gtaattttgg cttaaggata
     1801 gccatgactt tacccatatc agacattttt tcagcgccag tagacttgat ggcatcgctg
     1861 attaactctt caatttcatt gtcatttaaa ggttcaggca ggtattttga aatgacattc
     1921 aactcaaact gttcctgggc aacgagatcg tcacggcttg ctttttcaaa ttgagcaata
     1981 gattctttgc gttgtttgga cattttatcc aggatgacta acatgcgctc atcatccact
     2041 tcaatgcgct catcgacttc aatttgtttt accgcagcac tgattaagcg taaagcattg
     2101 agcaagtttt tgtcctttgc gcgcatagcg tctttaatgt cattgttgat ttgttctttt
     2161 atagtcattt taattcctta aaaaacaaaa ggtcacatac aatgtgacct tggtgtaata
     2221 taggacgagc tgcgatttac taccgaaact gccacgaagg ccaatttcaa ataaaaacaa
     2281 ttaaaaataa cagagcttcc aaaacagggc actgtttaag tgcctcacgc aaacttgctt
     2341 tttagtgcag tcaatcttga aaaaatcaga tcaggtagtg ctttttttac gtcggtgacc
     2401 aacacctcgt cttgaactaa catcacgaga tattttttta agatgacgtt ttactgcagc
     2461 agcttgcttg cgcttacgct ctgcggttgg tttttcataa aactcacggc gacgtaattc
     2521 ggttaaaata ccggcttttt cacaagagcg tttgaaacgt cgtagtgcat attctgggtt
     2581 ttcgccttct tttacacgaa cggtaggcat taactagtcc tctggttatt taatcttaat
     2641 aggtgggcaa ttctagtgct agattttcca cacgtcaaga ttaatctata cgtttcttgc
     2701 tattttagca aattatcaag acattgttct ttaatttaat ggtatctttt gtctgtgata
     2761 taattgaaaa atgattaaaa tctggaaaat tgattccgat tattgaggta gttcatgctt
     2821 gtattgggta tagagtcttc ctgtgatgaa acaggtattg ctgtctatga ttcagaatca
     2881 ggattattgt cccacgcctt gcattcacaa attgccactc atcgtgtaca tggcggtgtt
     2941 gttccagaac tggcatcaag agatcatgtg aattacttgg ttcctttagt cgatgaggta
     3001 ctaactaaag cgcaaattcg taaaaaccaa ttggatggaa tagcctacac cgcaggaccc
     3061 ggattaattg gcgctttgtt agttggttcc tgctttgcca aaagtctcgc ctacgcctta
     3121 aatattccgg cattggcaat tcaccatttg gaagcccatc tgttagccgc aaaaatggaa
     3181 accccatccc ttgattttcc ctttattgct ctgttagttt ccggtggcca ttgtcaacta
     3241 atagaagtaa acaacattgg tgagtatcga ttattagggg atactctcga cgatgcagta
     3301 ggagaagcct ttgacaagac agccaaactg atggggattc cctatcctgg aggagccgtg
     3361 ttggcaaatt tggctgatca atgtttatca actccctatc aatttccccg ccccatgact
     3421 gacaggccag gcttggactt tagttttagt ggactaaaaa ctcatgcctt aaatacctgg
     3481 aaccaaagtg aaaaaaaaga aagcgaccgc tccgaaattg caaaagcatt ccaacaagca
     3541 gtagttgaaa cactaattat aaaatgcaaa agagccatta aagagtctca atcaaaacgc
     3601 cttgtggtag ccggtggagt aggggccaat aaagccttgc gtagcgcttt gcaaaaatgg
     3661 attaaagaca tcaaagggga agtgtacttc cccgctctgg aatattgcac agacaatggt
     3721 gcaatggtcg cttatgcagg atgtttgcgc atggtgcggg gggagagtga tggggggttg
     3781 ggggttatgg ttaaacctcg ttggcccctc gcttgaacgc ccgtcttcgg cgcatcttgc
     3841 cagatcattg ctctaaaaaa tgctcatgta ctcatcttgt acactccgct tttttagtcg
     3901 caacgatctg gcaacctgca ctcgaatacg ggcgttgaag ggtaggtttt ctttggcgca
     3961 tctttgcttg tgcattaaat aagtgctgcg ccttattcct tttctttgac tgttttcgtt
     4021 ttaggtttgg ttgatttcgg ttttttagtt gtttcttcct tgctggttgt cttttttgct
     4081 tttgttgttg cttttttggc ttttggcttt tttacagcct tagcttgttc tgttttttct
     4141 gtttcatcaa tattcgtatc aatgacttct ttgccaggaa actcctgctc tgcagaagtc
     4201 gctggagaag cttccattat ttcctctatc acactgtgtt tgaacttgat tttaggctct
     4261 tttccatcta tcaaacgatt aaaattatct ctgtgtttgt agagaaccag aatggtgatc
     4321 atgaataatg gggggaaaat attaagatta cccactaata ttaaggagta aaaaggggct
     4381 aaagagatag aagctattga tgctaaagag gaatagcgcc agaaatttgc taccagtaac
     4441 caggtcgcgg caaccatgac accaattaca aaatgaaatg ctaacaaggc tcctattgtt
     4501 gtagcaacac cttttccccc tctgaaatga aagaaaacag ggtacatatg accaacaact
     4561 gcagctaaag cagtaaaggc aacggttaca ggttcggcac ttaaaaattt ggccaggatg
     4621 acaggaagta taccttttaa agcatcgaat accatgacca taataccgta ctgcttccct
     4681 ccaagacgga gtacattagt agctcccgga ttttttgatc cttcctctct tgggtcgggg
     4741 agcccaaacg tacggcacac tatgatagct gaattaatgg agcccattaa ataaccaact
     4801 agtattagaa atataaaaag cgccaccgat ttctccttac tcgggaataa tttttattat
     4861 caaccaatct caattaacag gcaattttaa gatagaaaat atgccaagac tagataatag
     4921 aattcatttc atgttatttc aatttaattc catgattagt gcttgaatga gtgtaggaat
     4981 tgacttgttt atgcaaacag caggcatggt tggtataatt tgatccaaca agagcgttca
     5041 tattgctaaa aaagaggatg ttccatagaa tttgaattat ttgcaatagt ttgagacgga
     5101 tactgaatgt cgccttttag tcctgttcgt tgtgtcacta tcattctatt ccattatcta
     5161 agggaatttt actaaagcta tggatggcat tcatctcctt tctgcaatag aaatcattcg
     5221 ccgcattaaa atgaaagaac tctcggctgc agaggtcatg gtggcgcact taaaccatat
     5281 tgataagatt aatcctgtaa tcaatgcgct caccgaacgt attccccctg aggaatgcct
     5341 aaagcaggca aaggaaattg acaagagtat tgcctcgaaa aaaaatctga acaagttaat
     5401 ggggttgcct gttgccataa aagatgcttt gtatgtgaaa ggactaattt gttcttcggc
     5461 atgttcagga ttttacaagg gagaaaaagc agtacgtgat gcgaccctgg tttctcgact
     5521 gaaaaaggaa ggagcaatta ttcttggctt aaccaatgtt cctgaattgt gtcgcggtgg
     5581 tgattcggat aatttaatct atggacggac caataatcct tatgatctgg caagaaccag
     5641 tggaggcagt agtggtggtt ccgcggcttt gatcgctgca ggaggcgttc cttttgcttt
     5701 gggttccgat ggtggaggta gtttaatgca gcctgcccat tgttgcggca ttgttgcatt
     5761 gaagccaaca catggccatc ttcctcatac aggctctgta ggtggggatt cctatggtct
     5821 tattggcaat ctgatttctt ttggaccaat ggctcgttct gtcagtgacc ttcgtttagg
     5881 actctccgtg cttgcgggct ctgaccaata tgatccttat acaaatccgg ttcctgttat
     5941 gcctgctgca ccactaaaaa aattacgtgt agcctatttt acagagaatg gttttacgcc
     6001 agttgatgcg gagatacaga acgttgttaa atcagcagcc ttggctttgc aggatgatgt
     6061 ggctatagtg agggaggtac gacctgattg tgtcagcaag gcttttgatt tgcactggga
     6121 gctttttctc gggggcgatc gcggagcggg atttaaaact atgttatcgg agttaggggt
     6181 taacaatctg tcatgggaac tacaagagtt cttgcgtcaa gcggaacaaa ctcaattctc
     6241 ggtgactcaa ttgcatcaac gtatgcgtga aatcgatttg tttcgtctgg aattagcttt
     6301 attcatgcaa gattatgatg tattgattag tccagtgttt ccaacagtag caaaaccgca
     6361 tgggattggg attaaagaaa tcagcgattt ttcctatgcg atgacacaca atttaagtgg
     6421 atttccaacg atcagcctaa gatgtgggac ttcagccgag gggttgccca ttaatatatt
     6481 aatagccgct aatcgctgga aagatactac ctctctcgct gttgcagaac gccttgagca
     6541 attaatgggg ggatataaac ctccttctct aatccactct ctgcaatgaa gcaagaatca
     6601 ataatttttg aaacagcgaa ctagggcaga aacaaggctt gcaaatcatt agtatattgc
     6661 ctgcctaatg gtgttacttg ccaatgagta tctgttaaat taatcaattg tttcttatta
     6721 gccaatttca gcaagggtaa aagctgatta agttccaatc ctgtcgctgt cgtaaataac
     6781 tccaaaggta tggattgttc cagccgggtt gtattcagca taaactcaaa cagcaattct
     6841 ttacctgtaa tggtttcgcg agcagccaga taaggtttct cattatccag ataatctttg
     6901 ggttgacggt gcttacgggt tcttattatt tcgtttgggg ttgtcatttt tccatgagcg
     6961 ccagcgccta tacccaaata atcaccgaat agccaataat tgatattgtg tctggcttgc
     7021 ttttccggtt tggaaaaggc agaaatttca tagcggtgat atccggaatt atgtaacagg
     7081 gtaaatcctt gctcctccag caagtaatct tcttcttcag atggcagggg aggagtatgc
     7141 ttgtaaaata cagtattcgg ttctatggtt aattgatacc aggataaatg ctcaggttga
     7201 taggataaag cggtcttaag atcctgtaat ccctgcgtta cactttgatt aggtaaactg
     7261 tgcatgatat caagattgag attatcaaag ccggctttac gggcagtatc tatagcagaa
     7321 tgggcttgtt tctcgtcatg aatacggcca agaattttta aatgattggg attaaagctt
     7381 tgaattccca aagaaagacg attgattcct gattgtctat aatcagtaaa tcgatgttgt
     7441 tctacagtac cgggattggc ttccattgtg atttcaatat tttttgcaaa ggggagtatg
     7501 cgctttaatt cattaaatag gttgttataa gcttcggctg aaaataaact gggtgtgccg
     7561 ccaccaataa aaatggagtt aatttcacgg gtattgtatg aggaaatatc cgttttaagg
     7621 tcttcaatca aagcctgaat atactggtgt tcgggtaaaa catcagggct tttgtgtgag
     7681 ttaaaatcac agtatgggca ttttctgatg caccagggaa tatgaatgta caaggataac
     7741 ggaagcatta caatttttaa actggcttgt tagggaatgc aatagtatca tggtagcaat
     7801 tttgctacaa aattaagtga tgaccattga tgcgaaataa accaaaatgt aagcaaagga
     7861 ccgtgatagc caaatcccgg ttgtttacag ttgaagaaat gtatctggaa ttttccaatg
     7921 gggctcagcg agtctatgag cggattaaaa gtcatggtca tggtgcggtg ttaatagttg
     7981 cattgactcc cggagaatca ttattattag ttcgggaata cgccgcaggt actgattctt
     8041 atgaattatc atttcccaag ggagtgatag acagaagtga aacccctata gaagcagcta
     8101 acagagagtt acgtgaagag actgggtttg cggcaaggga actgcactgg attcgttcca
     8161 tgtcattggc tcctggttat tttggagctc gcattgagct tattgttgca gagggtttgt
     8221 accattcgcc gttgccaggt gatgagcctg agcctcctga aacagtagag tggcctttac
     8281 acgctgctta tgagttattg gaaaaaccgg attttaccga ggcgcgcagt attgctgcgc
     8341 tatttttagt taaagagtgg ttgaataaga ggaaatcata tgacaaataa tcgagtaaga
     8401 gttgctgtca caggtgcagc agggcaaata ggttatgccc tggtgtttcg tattgcttcc
     8461 ggtcaaatgt ttgggccaaa tactgaagtg gaattgaatt tacttgaact tgagccggca
     8521 cttccctcac tggaaggagt cgctatggag cttgatgatt gtgcatttcc cttattaaaa
     8581 cgcatagttt gtactgcaga tttgaataag gcaatggatg gtgttaactg ggcattatta
     8641 gtcggttcag ttccgcgaaa gcaaggcatg gagcgttctg atttattaca aatcaatggt
     8701 ggtatattca ccaaacaagg tcaggcgatt aatgattatg ccagcgatga tgtacgggta
     8761 tttgttgtag gaaatccttg taataccaat tgcctaattg ctatgaatca cgcaaaagac
     8821 gtgccttctg atcgttttta tgcaatgacc acattggatg agttaagagc ccgtactcaa
     8881 ttggctaaaa aagcaggcgt tgacattacg gcagtgactc aaatgacgat ttggggtaat
     8941 cattccgcta cccaatatcc cgatttttat aacgcaaaaa tcaatggaac ttctgctgcg
     9001 caagtcatta atgatgaaac ctggttaaaa gagacttttg tatcaaccgt ccagcaacga
     9061 ggagccgcag tgatcaaagc gcgaggctcg tcttctgctg cttctgccgc caatgcgata
     9121 attacagggg ttaatcattt ggttactgat actcctgctg gagaatcgtt ttcaatgtgc
     9181 cgtcgctcaa agggagagta tggagttgat gaaggtttga tcttctcttt cccatgccgc
     9241 agagaacatg gcgaattaaa agtggttgag aacttggagt tcaacgattt tggacgcgag
     9301 agattcaata ctactttaaa cgaattacga agtgagcgtg acacggtcaa gtctttaggc
     9361 ttattggatt aaccaaaccg ggtcaattct tgatagattt gattctgttg tttttgctga
     9421 ttaccctgct ttgtgaattt ttcagggcag ggtgctgtcg caccttgttt tataaaccag
     9481 gtttcaatga ctcttttgtt tgtaaatcag ggtctttatt gggatgttga tgtctgtttt
     9541 tatattcctc actcaaatac atttgaaata atggcgaatt ctccaggatg gttttggcgt
     9601 ctgtgtttcg taaaagagtt gatacggaac gaccattgga taatatttcg ttgagagtat
     9661 gaatcaattg ctctatttgc tcgaagggtt cttttctttt atgcatcaaa acgggaacat
     9721 gcttaattat agctctggca agttgttcgt ctgtcaagta aagaatcaag cgggtttgag
     9781 aaattttgat gaaatttctt tctgatctga gaatgatatt gtataagaaa tcaggatctt
     9841 tttttaagtg attgctcaat ttttggctaa atccaagcct gtctcggttg tcaaccagtg
     9901 tttgggttaa ttcagtatga tccaattgac taaagggttt tcctaccttt ttttcaagcc
     9961 aggtcattaa atcatgctca tgatgagaat gattcagtgc gctgaattga aatcgtggcg
    10021 atacttttgg atgagataag acctgattcc agtcaatagt tggcatgtgg tgcaatttag
    10081 ccagagcatc catggatttt ctgattacat taaaattaat ccgccatttt tcaatataag
    10141 gcaaggcttc tctaattgct ctaagcagtt caataaaagt atttctggct tttggattgt
    10201 agttctcggt ggcttcttta agatgttgaa ctgctggaaa agtgtacttg ccatttctca
    10261 ttgcctctaa atgaaatact gattgtaatg attttagcaa tacagaaaaa gggtcttcca
    10321 taccaatatt cctccacagg cccttattta aaagtagttt ataaaaacat aaaagccaaa
    10381 tattttgcaa aatggattaa taatttttag tttgattgac tttgtttatc gtattgataa
    10441 aaacaattga ttttatttat taaatgattt cacctaaatt taacaatgaa aatagtgtta
    10501 atttaacgag gagaaaactt atgattacgg taggcaacaa atttccagag tttcaactta
    10561 aagcaacggt ggctaatgat ataaataatg catttcaaat gatctctaat gaaacgtatc
    10621 ggggtaaatg gttagtcgta tttttttggc ctaaagattt tacctttgtc tgtcctactg
    10681 aaattgcgga gtttggaagg ctaaattccg agtttgccga tcgagatgct caaattctgg
    10741 gtggaagtac agacagtgaa tttgtacatt tggcctggag aaatcagcat ccggacttaa
    10801 aagatttgcc atttcccatg ctggctgatg ttaaacgaga attgacagca aatttgggca
    10861 tattggatga cgaggagggg gttgctcaac gagcgacatt tattgtagat ccccagggaa
    10921 ttactcgttt tgtgatggtt acggatttaa atgtagggcg caatccagct gaagtattac
    10981 gtgttctgga tgctttacag accgatgaac tttgtccttg taattggaaa aaaggtgaag
    11041 aaacaattca tatcaaataa attgaaaaaa gaggcatcaa tcatgttaga gaatgttaaa
    11101 gaaagtatac cggaagtggc taaagacata cgcttgaatt tgactaaggt acttgatttg
    11161 gaacaaagtg atggattgag tgagcatcag attttaggtt ctgcattggc tgtgagttat
    11221 tctttaggca atgaggttct aattaaagga attaaagaat tggtgcataa cgaacagatt
    11281 gtgcgtgcag cacaacttgc agcaagcctt atggcgatga caaatattta ctatcgcttt
    11341 gttcatttaa ctgaaattaa ggaaattgag catattcctg caggattgcg aatgcaagga
    11401 atgatgactc caggagtaga cagggtaacc tttgaaatat tgtcccttgc tgtttctgct
    11461 ctcaatggtt gtggggcttg tgtcagctcc cacgcacgtc aattgaaaga gcacggctta
    11521 tccgatacga gccttgcacg agttggacgt attactgctg tgattcatgc ggttcatgtg
    11581 agtggtcgaa tcaaataaat catcgcattt gaagcagatt atctgcttca aatgcaaaaa
    11641 actatttaat gacgccgcaa gctatacggt cccccccgcc accttgtgga ggattgtcac
    11701 tgtaagtgtc tccattggca tggatcatga cagctaaatt gtgcatatca cttaatttca
    11761 aacgaggagc taaagtaggt atcattgctt tcccattact tgtgacatat aaaacaggta
    11821 aatctcccaa atgcccgtta ccataagggc cttggtggct attggtattt tgaggatcat
    11881 aatgtccttc tgcatgcatt ccgtggttac cacaatctgc atttttatgt aaatggaaac
    11941 catgcggacc ttcaggaagg ttagttaagt ctggtgtaat caaaacacca taaggggttt
    12001 gtgtgaaggt tactttgcct attgcaactg gtttgggacc agttgtgtaa attggtgctg
    12061 taagatcatc ggcaatagcc atgcttgaga ataaaatagt accaattaat attattcctg
    12121 atttattcat gtcatttcct ggtatttttc aaa
//
LOCUS       DASLOE010000087        12123 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.088, whole genome shotgun sequence.
ACCESSION   DASLOE010000087 DASLOE010000000
VERSION     DASLOE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12123
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.088"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..658
                     /locus_tag="U6309_001844"
     CDS             <1..658
                     /locus_tag="U6309_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN5505283.1"
                     /translation="DTFNPGNDNLFLKTASVGTGEYFILPRLCAHLAKNAPKFVLKIS
                     SLSEYLSLEYLLMSELDFAIGPGFVETGNNIIRELLFEEEAVCVMHKSHPLANQELTQ
                     DMYLQAEHVDIQFSYMGNENILYKALQGYHQRNIKVIVPNIISALEVVYHTNFITTVP
                     RTLAVTFKDRYQIELHPFPFPKKTFLVNFYYHKRLSNHKPLQWVLDVIKKYCCVNHEA
                     "
     gene            complement(670..1257)
                     /locus_tag="U6309_001845"
     CDS             complement(670..1257)
                     /locus_tag="U6309_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEN5505284.1"
                     /translation="MARNDPITFLTTWISEELERGAPNPQHATLSTTGKDSIPHGRVI
                     AIREINEQGLLFFTQRGTRKVVEMTYNPMVSLTFWFELFEREVMIDGSARPLSESENQ
                     AYWDTYPPFAQIRFHSYAPTSSQVIPSKQIIENKRKILTSQFHDKPLPMSEYYCGFRI
                     IPTRMVFYTYRTDELSDVVEYCFIKNNWEKNLLSP"
     gene            complement(1391..1771)
                     /locus_tag="U6309_001846"
     CDS             complement(1391..1771)
                     /locus_tag="U6309_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN5505285.1"
                     /translation="MVQIIEMDEKKLIQKKAPIKTASMVEAIYGCKWSLTIYSLLRNG
                     INRPGEMVRSIEGLSTKVLNQCLRKNIEFGILGRNTFHEIPPRVEYFVTPFGEKFLKI
                     LDQIEELNEQIRDYQQIEFDNNDS"
     gene            1866..2630
                     /locus_tag="U6309_001847"
     CDS             1866..2630
                     /locus_tag="U6309_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN5505286.1"
                     /translation="MFNQNLAIIIGGSSGMGLETAKKLSSNNMELLITGKDKQKLENA
                     AAEIQKLGAKVDILVLDLYDKEQVFQFMSSMNNETRRVKYLVNAAGYFKPITFLEHEE
                     EDYDRQVNLNKAFFFITQAVVKNMKKNGGGSIVNIGSMWANQAVKATPSSAYSMQKAG
                     LHSLTQHLAMELAEHNIRVNAVAPAVVATPIYKSFIVEERIEESLQDFNKFHPIGRIG
                     KATDIADAIEFLLSEKASWITGEIMNVDGGVMAGRN"
     gene            2713..2913
                     /locus_tag="U6309_001848"
     CDS             2713..2913
                     /locus_tag="U6309_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505287.1"
                     /translation="MWALDKDYIRSSQYPESVWAEEMLKHRLEALARKIKFHTRLRDG
                     INASSARAIQNNGCLIHNSRYP"
     gene            complement(3007..3948)
                     /locus_tag="U6309_001849"
     CDS             complement(3007..3948)
                     /locus_tag="U6309_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEN5505288.1"
                     /translation="MILNITAAQFPDLTLNAIESSQNIYRSIDFNFGEDADTAINKAS
                     LEKFINQFKSIHSTHDKPIEGIITVGKMKNVSPDTVKLLLTTEDFVQMLDQKSFLKLI
                     VTSNEIANFVLDNPKLRAKLDGIEPLVDAQKFENSCTARAIMKILLERGLIEPSSYTP
                     SKELEIYKDIWLEPGKVASPEKIASYFCKYNLDVIGVEIRELSKSVRNKYSKDMVITS
                     LYSLFKKEVPIRKKMTLTTLSEADFPEGITTLIIIKAGVLHTLLGKKHHGQFEVTDPW
                     FGDKKIYSGFMDFLEKERKNLGVFFEISQGSQEIFRP"
     gene            4182..4700
                     /locus_tag="U6309_001850"
     CDS             4182..4700
                     /locus_tag="U6309_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-box protein"
                     /protein_id="HEN5505289.1"
                     /translation="MKKNFFSALPEETIINTLSFLKAKTLGRVAQTCQFFNRLANDKH
                     LELNQIRQQRIKQELWGNLMVAARSNNLEEVKKILKKGIDPTQTNNYHLNRTPLLAAI
                     EGRAYQTANYLWRKYTFDPNFKDNYGDSPISLLKKQLANPAFKDKEKKQIRALIREMQ
                     EEKIAQSKCLVC"
     gene            complement(4731..5150)
                     /locus_tag="U6309_001851"
     CDS             complement(4731..5150)
                     /locus_tag="U6309_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN5505290.1"
                     /translation="MRAKNDECTHVLLVDDDEIDLMSIQRALTKLNIPVCFHIARDGV
                     EALEQLSGNIKVTKPKIIVLDIMMPKMNGIEFLRELRSHIHYNPIKVVIFTTSSNIRD
                     KQATTGLDIAGYFVKDSQFNEFVYCCKHLLEKKSHLS"
     gene            complement(5140..7590)
                     /locus_tag="U6309_001852"
     CDS             complement(5140..7590)
                     /locus_tag="U6309_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN5505291.1"
                     /translation="MRRENTKSNIDFKSIFDSLPDLYLLLDRDFNIVGVSDAYLLATM
                     VSRKQILGKNVFDAFPDNPNDPKATGAKNLYASLLRVLKNKSADTMAIQKYDIQRPAD
                     RGGGFEERYWSPINAPVLGTDQEVEYIIHRVEDVTEFVHLKKTGSAQLKLMEELRTKA
                     GEMEIEIYQRAQEIQEANKQLEEANKNLARLDQIKTQFFANVSHELRTPLMLILGPLE
                     MLLAEKKLPSFVQNTLRRIQNNAQILLKHVNDLLNISKLDAGKMILHYANIDLSKLIR
                     QTIALFEAQIPERKLVFSMDLPDELQAQIDPDKIQHVLINILSNAIKFTPQNAKVRIR
                     LVQINPEICRLQIEDNGPGIPSDLCETIFERFFQIEEPTTRTVGGTGLGLSIAKDFVE
                     LHGGSILAGNARNGGAIFTIELPLKAPQNVKVYSSYSQLLLGEKEISASLAEFRNKVK
                     PIKEGAEDDFEQDYYDRPLILVVEDNQEMNHFICKILNTFYRTESAFDGREGLQKSLA
                     LHPDLIVSDIMMPHMSGVEMVHAIREHPLLLKTPIIILTAKADDELTARMLEEGAQDY
                     VIKPFSAKEFKARVANLILVKKAEDELDQFVYHASHDLKAPLPAMEHLISWIEEDLGE
                     MLSEQSKKYLLLLRQRASRMSKLLDGLLKYAQSGYVFDKVRTIHTDRLITDIIKKLNP
                     PQTVTFQLEDTLPVLNTCELPLREVFTALLDNSIRHHHRKDGHIIIGTKEKDRFYEFF
                     VADDGPGIEKKYQDRIFQLFQTLQPRDKLETSGVGLSIAKKIVEFQGGKISVESEKNK
                     GSVFRFLWPKDKEIGDAC"
     gene            8004..9452
                     /locus_tag="U6309_001853"
     CDS             8004..9452
                     /locus_tag="U6309_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505292.1"
                     /translation="MTTSKLEKTGLRVHEKIKHMVKSYGTMITGIPAEILGQNEAEIS
                     VGYVKKMGNMKENIAEVVRKSEMTQPTNSCGQASNEICALLLGTEGASEFEKTSYEIL
                     SEDGSNLKSSLPNKNLLVRVEVDRFNSPHQYQKIKREKFNPETAEKNKIYLLEDQLLY
                     LDIFGKVIDLGQTSDTCQKLFDAITSPTYQNYILNDEFIEPEDGDEEAAVCEISHIVT
                     AKMKNMDCWTSTHSFTIFVPESSSEDTRTLYPYQAYWTSHTLQQWFSGDKDEKLSRLG
                     IDGYIEKLALLGTTTDSKIRSSIYGELFSPPGKEHVFCTGMNEKFSPLRVKFKVTEVE
                     PKIALQNLQEVQEFIDTNYPGENAKAQCELYKTRAQDAMTKQLEKRLLIEPTHPKESV
                     TPYKEKNTQSFFGKNLDTTSRIDKMISSVITMENLKILAKEADTLSGKDREKLVEYFK
                     TLPSEQLAEIKPESETAQKAWDEQYGLVLRDK"
     gene            9528..10808
                     /locus_tag="U6309_001854"
     CDS             9528..10808
                     /locus_tag="U6309_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2148 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505293.1"
                     /translation="MIVGGINMTKIKLESPGFMVHKKLKSMYHSYGVMMTGVPAEVLG
                     QMQAERSIPSINKTGNLKQQIAKEVSKVCHLMTEPTQSCGQASNDVCELLLGKIEAEK
                     FHFTKYEALSADGDNLKNALENTVPSSTNLLIRFEIDREDPPIVLVKTKNENFNPETA
                     VRNKIYLLENKLYFLDKMGNLFNLGPGKKKCTQLFNAIGDSAEYSLCDPFVLEEPEKP
                     EDFAISEIVDIFNEQKERFDFWIGSHSFTIYIPQTLGESPRQFYPYQAYFGSHTLQDW
                     FVSDKDEYLSQIGIDKYIEKLAVLGKTTNTKERSDIYAEFFSKRGREAFFCAHLNEKR
                     QPLRVKFKITEINPELALKNLQETQEFIDTHPGENPSDKVENYRNRAKLAMTEHLESL
                     LDIKPESSPSLFWKNVDKKEQEMTDSSRYKKGPS"
     gene            10896..11255
                     /locus_tag="U6309_001855"
     CDS             10896..11255
                     /locus_tag="U6309_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2149 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505294.1"
                     /translation="MMSREKAAGTFFKDYQKKNVMRLLQDSLEKIINEWLKTDDESHT
                     KLKSLQELSEMDINATSFAEPSPLPDFVTRLWLDPHKALDAMDKNISKNEIRKLIKET
                     AKEIELVFTHQKAPDYS"
     gene            11489..12070
                     /locus_tag="U6309_001856"
     CDS             11489..12070
                     /locus_tag="U6309_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505295.1"
                     /translation="MSCYILDLIETLKNDFVKKNTPTRFDSVKSYGELLEKSKINPAI
                     AEYITGTSSGVMMVDSYEKLLELSKINCAVADNMTQTQLGLDLIDSYEKFLEITKINA
                     KVTFNFQILNYQRYAKLVDSYQQSSQAKSLSGLNTSANSQLFFKKRDSTGQDRVSITT
                     GQAGVAHDTVGLGLEWNEAIQNTISENMMNQKL"
BASE COUNT         4032 a         2260 c         2154 g         3677 t
ORIGIN      
        1 agatactttc aatccaggaa atgacaattt atttttaaag actgcatcag taggtactgg
       61 agaatatttt attttaccca ggttatgtgc tcatttggct aaaaatgctc cgaaatttgt
      121 attaaagatt tcttcactgt cagagtactt gagtctcgag tatttattaa tgtccgaatt
      181 agactttgca ataggaccag gctttgttga aacggggaat aacatcatca gagaattatt
      241 gtttgaagag gaagccgtgt gcgtcatgca taaatctcat cctctcgcaa atcaggaatt
      301 aactcaagat atgtacttgc aagctgaaca tgttgacata caatttagtt acatgggaaa
      361 tgaaaatata ctatataaag cgttacaagg atatcatcaa agaaatatca aagtgatagt
      421 gcctaatatc attagcgcat tggaagtggt ttaccatacg aatttcatta caactgtacc
      481 gagaactcta gctgttactt ttaaagacag atatcaaatt gagctacatc ctttcccttt
      541 ccctaaaaag acatttttag ttaattttta ctatcataaa cgattatcaa atcataaacc
      601 gctgcaatgg gtattggatg taataaaaaa atattgttgc gtcaatcatg aggcttaaac
      661 aaattgatat taaggagaaa gcagattttt ctcccaattg ttttttataa aacaatattc
      721 tacgacatcg gacaattcat ccgttctata agtataaaaa accatgcgtg tgggaatgat
      781 tctgaaacca caataatatt cactcatagg caatggctta tcgtgaaact gggaagtcaa
      841 tatttttctt ttattttcaa tgatttgttt gcttggtata acttgtgatg aagtaggcgc
      901 ataactatga aatctgattt gcgcgaaagg cggataggta tcccagtatg cttgattttc
      961 gctttccgat aatggcctgg ctgaaccgtc tatcatgact tctctttcaa ataactcaaa
     1021 ccaaaaagtc agagatacca taggattata ggtcatttcg acaacctttc tggttcctcg
     1081 ttgggtaaaa aataataacc cttgctcatt gatttcgcga atggcgataa cacgcccatg
     1141 gggaatagaa tctttgcctg tcgtagataa tgtggcatgt tgtggattag gtgctcctct
     1201 ttccaattcc tcagaaatcc atgttgttag aaaggtaatg gggtcgtttc tggccataac
     1261 ttgaatacct attgtaaata ttttctggcg ttaataatga tacggactag cgatgtctca
     1321 ttacattggt gaatattgtg agccataatt cacttgcaat cgattagcaa taggtctttt
     1381 ataacaaatc ttaactatca ttattgtcga attcgatttg ttgataatcc ctgatttgct
     1441 cgtttaactc ttctatttga tccagaattt ttaaaaactt ttctccaaaa ggagtcacaa
     1501 agtactcaac tctggggggt atctcatgaa aggtatttct gccgagaatc ccaaactcaa
     1561 tattctttcg cagacattgg tttaaaactt ttgttgatag tccttcaatg cttcgtacca
     1621 tttcccctgg tcgattgatt ccatttcgta aaagagaata gatagtcaag gaccacttac
     1681 aaccatagat agcctctacc attgatgcag tcttaatggg agcttttttt tgtattaatt
     1741 ttttttcatc catttcaata atttgtacca taaagtactt agtgaactga aaagtgctta
     1801 ctttttttaa tattgtagat ctttcatact ggattggaca gtgttttaat taatcaggag
     1861 aatccatgtt caatcaaaat ttagctatta ttattggcgg ctctagtggt atgggccttg
     1921 aaactgcaaa aaaactttca agtaacaata tggagctgtt aattaccgga aaagataaac
     1981 aaaaacttga aaatgctgcg gcagaaatac aaaaactggg agcaaaagta gatattttag
     2041 ttcttgatct ttatgacaaa gagcaggtat tccaatttat gagcagtatg aataatgaaa
     2101 ctcgaagagt aaaatatctt gtaaatgccg caggttattt caaacccatc acttttttag
     2161 aacatgagga agaagattac gatcgtcagg ttaatcttaa taaggcgttc ttttttatca
     2221 ctcaggccgt agtaaaaaac atgaaaaaaa atggaggggg tagtatcgtc aatatcggtt
     2281 cgatgtgggc gaatcaagcg gttaaagcaa ccccctcctc tgcttattcc atgcaaaaag
     2341 ccggacttca ttcactaacc caacatttag ctatggagct tgctgagcac aatataagag
     2401 taaacgccgt ggcaccggca gtggtagcca ctcctatcta taaatcattt attgttgaag
     2461 agagaatcga ggagtcgtta caggatttta ataaatttca tcctatcggc agaatcggta
     2521 aggcaacaga tatcgctgat gcaatcgaat ttcttttgtc tgaaaaagca agctggatca
     2581 caggagaaat tatgaatgtc gatgggggag tgatggcagg gagaaattaa tcagcaaacc
     2641 ttatccagat ttatgcctgg gagcatcaat agcaagcgat tgatttgtaa tgccttgttt
     2701 cagatttatc tgatgtgggc tctggataag gattatataa gaagcagtca atatccagag
     2761 tccgtttggg ctgaggagat gcttaagcat cgtctcgaag ccttggcacg aaaaattaaa
     2821 ttccatacaa ggcttcgaga cggcataaat gcctcctcag cccgagcgat tcaaaataat
     2881 ggctgtctta tccataactc acgttatcca taaaaatcaa tcttttgcta ttgatgttcg
     2941 cactaaggct tttcattgct acatggaata atttggccat ttgcttttgg caaacattga
     3001 taactattac ggtctaaaaa tctcttggga tccttgagaa atttcaaaaa atactcccag
     3061 attttttctc tctttttcaa gaaagtccat gaatccacta tatatttttt tatcaccaaa
     3121 ccagggatca gttacttcaa actgcccatg gtgtttcttg cctaataaag tatggagtac
     3181 tccagctttt ataataataa gagtggttat cccctcaggg aaatctgcct cacttaaggt
     3241 tgtcaatgtc attttttttc ttattggcac ctctttctta aaaagtgaat agagtgaagt
     3301 aataaccata tcttttgaat atttatttcg tacagatttt gatagctctc taatctcgac
     3361 accaatgaca tccaagttat atttgcaaaa ataggatgcg atcttttcag gcgatgccac
     3421 tttaccgggt tctagccaaa tgtctttgta tatttccaat tctttgctag gtgtatagct
     3481 gcttggttca ataagccccc tttcaagaag tattttcatt attgctcttg cagtacaaga
     3541 attttcgaat ttttgtgcat cgaccaaagg ttcaataccg tccagttttg ctcttagttt
     3601 aggattatcc aatacaaaat ttgcaatttc attggaagtt actattaatt ttaagaaact
     3661 tttttgatca agcatttgaa cgaaatcttc tgtagtaaga agtagtttca ctgtatcagg
     3721 ggagacattt ttcatctttc ccacggtaat aatcccttcg ataggtttat catgagtact
     3781 atgaatactc ttaaattgat tgataaattt ctccaaactg gctttgttaa tcgccgtatc
     3841 cgcatcttcc ccaaaattaa aatcaatgct tcgatagata ttttgactgg attcaatagc
     3901 attaagcgtt aaatccggga attgcgctgc tgtaatattc aaaatcataa taacttgtct
     3961 tcacatcccc aaacccacac taacttgatt ataggttaat ttgaacacaa ataattcatt
     4021 atgatatata aaaattcaat cgtatcttgt atttgcctat tctgattctg ttctgctttc
     4081 attaaatcct ggttagcaaa aaagtgaagc aatgacttaa tattcaatta ataatcataa
     4141 tgtaaaatta taaaactctt taaccataac tattgactcc tatgaaaaaa aatttttttt
     4201 ctgcccttcc tgaagaaaca attatcaata cattaagttt tttaaaagca aaaacactgg
     4261 gccgtgtagc tcagacatgt caatttttta atcgtttggc taatgataaa catctggaac
     4321 tgaatcaaat caggcaacag cgtataaaac aagagctatg gggaaatctt atggtagcgg
     4381 caagaagcaa taacctggaa gaggtcaaaa agattttaaa aaagggaatt gatccgaccc
     4441 aaaccaataa ctaccactta aatagaacac ctttacttgc agcaatagaa ggaagagctt
     4501 atcaaactgc taattacctc tggagaaaat acactttcga tcccaatttt aaagataact
     4561 atggtgattc acctatctct cttcttaaaa agcaactggc aaatccagcc ttcaaggata
     4621 aggaaaaaaa acaaatacgc gccttaatta gggaaatgca agaagaaaaa atagcacaga
     4681 gcaagtgcct tgtttgttaa aggttttaaa ttaaacagat cgcgtctggt ctatgataga
     4741 tgtgattttt tttccagcaa gtgtttgcag caataaacaa actcattaaa ttgagagtct
     4801 tttacaaaat atcctgctat atccaaacct gttgttgcct gcttatcccg gatattactt
     4861 gatgtcgtga agataacaac cttaatcgga ttataatgaa tatgagaacg caattctctg
     4921 agaaattcta taccattcat ttttggcatc atgatatcaa ggacgatgat tttaggcttg
     4981 gtcactttga tatttccaga gagttgttcg agagcttcaa caccatcacg agctatatga
     5041 aaacaaaccg gaatattcaa ttttgttagc gctctttgaa tagacattaa atcgatttca
     5101 tcatcatcga ccaataaaac atgggtacac tcatcattct tagcacgcat cgccaatctc
     5161 cttatctttt ggccataaaa aacgaaagac gctgcctttg tttttttctg attccacaga
     5221 aattttacct ccttgaaact cgactatttt cttagcaatg cttagaccta caccacttgt
     5281 ttcaagtttg tccctgggtt gtaaggtctg aaataattga aaaattctgt cttgatattt
     5341 tttttctatt ccagggccat catccgctac aaaaaactca taaaaccgat ccttttcttt
     5401 tgtgccaatg atgatatgtc catctttacg gtgatgatgc ctgatgctgt tatcgagaag
     5461 cgcggtaaag acctctctca aaggaagctc gcaagtattc aatactggca gtgtatcttc
     5521 gagttgaaac gtgacagttt gcggaggatt caatttcttt atgatatcag taatcaatct
     5581 atcggtgtga attgtacgaa ccttatcaaa aacatatcct gattgagcgt atttaagcaa
     5641 tccatcgagc aactttgaca ttcgtgaagc acgttgacgc aagagcagta aatatttttt
     5701 agattgttca gaaagcattt cacccagatc ttcttcaatc catgagatca aatgctccat
     5761 agcaggcagc ggcgctttta aatcatgtga tgcgtgatag acaaattgat ccagttcatc
     5821 ttcagctttc ttcaccaaaa tcaaattagc aactctggct ttaaattcct tggccgagaa
     5881 tggttttatc acataatcct gagccccttc ttccagcata cgtgcagtta attcatcgtc
     5941 tgctttggca gttaaaataa taattggagt tttcaatagc agggggtgtt ctctaatagc
     6001 atgcaccatt tcgacaccgc tcatatgagg catcataata tcactaacaa tgagatctgg
     6061 atgtaaagcg agcgattttt gcaacccctc cctaccatca aaagcacttt cagtacgata
     6121 aaaagtgttc aatattttac aaataaaatg attcatctcc tgattgtctt ctactaccag
     6181 gatcagcggt ctatcgtaat aatcctgttc aaaatcatct tcagctccct ccttaattgg
     6241 ttttacttta tttctgaatt cagcaagact cgcgcttatt tctttttccc ccaataatag
     6301 ctggctatag gaagaataaa cctttacatt ttgaggcgct tttagtggta actcaatggt
     6361 aaaaatggca ccgccatttc gagcattccc tgccaaaatt gaacccccat gtaattcaac
     6421 gaaatctttt gcaatcgata aacccaaccc agttccccct accgtacgag ttgtaggttc
     6481 ctcaatctga aaaaaacgct cgaatatagt ttcacaaaga tcagaaggaa tacccggccc
     6541 attatcttct atctgaagcc tgcatatttc aggattgatt tgcactaatc taatccttac
     6601 tttcgcattt tgtggtgtga attttatggc atttgacaaa atattgatta aaacatgctg
     6661 gattttgtcg ggatctattt gtgcttgcaa ttcatcaggc aaatccattg aaaaaacaag
     6721 cttgcgttcg ggaatctgag cctcaaacaa tgcaattgtc tgtcgaatta attttgacaa
     6781 atcaatattg gcataatgaa gaatcatttt gccagcatcc agctttgata tattcagcaa
     6841 atcattgaca tgtttcagta aaatttgagc attattttgt atgcgcctta atgtattctg
     6901 aacaaatgag ggaagctttt tttcagcaag caacatttcc agaggaccta gtatcagcat
     6961 taatggggta cgaagttcat gactgacatt agcaaaaaat tgggttttga tttgatcaag
     7021 tctagccaga tttttattag cctcttccaa ctgcttgttt gcttcctgaa tttcctgggc
     7081 acgctgatag atttcaatct ccatttcccc agccttggta cgcagttcct ccatcagctt
     7141 cagctgcgct gaaccggttt tttttaaatg gacaaattca gtaacatcct ccacacgatg
     7201 aataatatat tccacttcct gatcagtccc taaaaccgga gcattaatag ggctccaata
     7261 acgttcttca aatccccctc ctcggtctgc aggtctctga atatcatatt tttgtatagc
     7321 cattgtatct gctgatttgt tttttagaac ccttagtagc gatgcatata agttcttagc
     7381 acctgttgcc tttggatcgt ttggattatc aggaaaagca tcgaagacat tttttcccaa
     7441 aatttgtttg cgactcacca ttgttgccag taggtaggca tcactgaccc cgacgatatt
     7501 aaaatccctg tccaataaaa ggtataaatc cggcagggaa tcaaatatgg atttaaaatc
     7561 aatatttgat ttcgtatttt cccttcgcac agagcatcct ccttcctcta tgtaaggcct
     7621 attggctata aagcatgacg caagtctttg cgaggctggc catgaatcca atgtgcaatt
     7681 ttcaacacag ggtatgaata gccaattaac cctactacta attataatcg aggatttgag
     7741 accttactaa ttaggaaagc ctggtttcga taattgcttg caaccaccaa caaaaagatg
     7801 aatctctttc agccattaca gcgcttgctt tagtggttcg atcccccctc tcaggaattt
     7861 ttcttcaata gtcgccctat cgatttaaga tcttatcgtc aaccaaaata taaaaaaata
     7921 caaaaaacca taaaaaaaac caaagtgtac aaaatttatt ttaatctaca attaaatata
     7981 gggtatctat cagtgaggtt aatatgacaa cttccaagct tgaaaagacg ggtttgcgtg
     8041 ttcatgaaaa aatcaaacat atggtcaaga gttatggaac gatgataacc ggcattcctg
     8101 ccgaaatatt agggcaaaat gaggcagaaa tttcggttgg atatgtaaaa aaaatgggaa
     8161 atatgaaaga aaatattgct gaagtagttc gaaaatctga aatgacccaa ccaacaaaca
     8221 gctgcggaca agccagcaat gaaatatgcg ctttgctttt aggaacagaa ggagcgagtg
     8281 agtttgaaaa aacaagctat gaaatcttat ctgaggatgg cagcaatttg aaaagttctc
     8341 tgcctaataa aaatttactg gttagagtcg aggtggatcg atttaactcc cctcatcaat
     8401 accaaaaaat aaaaagagaa aagtttaatc ctgagactgc ggaaaaaaat aagatttatc
     8461 tactggaaga tcaacttttg tatctcgata tatttggaaa agtcattgat ctggggcaaa
     8521 catcagacac atgtcaaaaa ttatttgatg ctattacaag ccccacttat caaaattata
     8581 tccttaatga tgagtttatt gaaccagaag atggtgatga ggaagcggcc gtttgcgaga
     8641 taagtcatat agttacagct aaaatgaaaa atatggattg ttggacatca acccattcct
     8701 ttaccatatt tgttcctgaa tcgagcagcg aagacacacg cacattatat ccttaccagg
     8761 cttattggac cagccatact ttgcaacagt ggttttccgg tgataaagat gaaaaattat
     8821 cacgcttggg tattgatggc tatatcgaaa aactggcttt gttaggcaca acaaccgata
     8881 gcaaaatacg ctccagcatt tatggagaac tattttctcc tccaggaaaa gaacatgttt
     8941 tttgtaccgg gatgaacgaa aaattctcgc cattacgagt caaatttaaa gtcactgaag
     9001 tggaaccaaa aatagcattg caaaatttac aagaagttca agagtttatt gatacgaatt
     9061 atcctggcga aaacgcaaaa gctcaatgcg aactttataa aacaagagcg caagacgcga
     9121 tgaccaaaca acttgagaaa cgacttctaa tagagccgac tcatccaaaa gaatcagtaa
     9181 ccccctataa agaaaaaaac acacaatctt tctttggcaa aaacttagat acaaccagca
     9241 gaatagataa aatgatctca tcagtcatta cgatggaaaa cctcaagatt cttgcaaaag
     9301 aagcggatac cctctctgga aaggatcgtg aaaagttagt ggagtatttt aaaacattac
     9361 catcagagca gctggcagaa atcaagcctg aatcagaaac agctcaaaaa gcctgggatg
     9421 aacaatacgg attagttctt cgtgataagt gataaatcaa atcagctttg tgtatttgtc
     9481 acttgaactg tttataatca aatgatattg acctataata tatcactttg attgtaggtg
     9541 gcattaatat gacaaaaata aaactggaat cgccgggttt catggtacac aaaaagttga
     9601 aaagcatgta ccacagctat ggagtcatga tgaccggtgt ccctgctgag gtcttaggac
     9661 aaatgcaggc tgagcgatct atccccagca ttaataaaac agggaacctt aagcaacaaa
     9721 ttgcaaagga ggtttcaaag gtatgtcatc tgatgactga acccactcag agttgtggcc
     9781 aagccagtaa tgatgtgtgt gaattgcttt taggaaaaat tgaagcggaa aaattccatt
     9841 tcaccaaata tgaagcatta tcagcagatg gagacaatct aaaaaatgcg ttggaaaata
     9901 cagttccatc cagcactaac ctattaatac gatttgaaat tgacagagaa gaccctccca
     9961 ttgtattggt caaaacaaaa aacgagaatt tcaatccgga aaccgccgtg aggaataaaa
    10021 tttacctatt agaaaataaa ttatattttc ttgacaagat gggtaatctt tttaaccttg
    10081 ggccaggtaa aaaaaaatgt acgcagttat tcaacgcaat tggagactca gcagaatata
    10141 gcctttgtga tccttttgtt cttgaagagc ctgaaaaacc tgaagatttt gcaataagtg
    10201 agatcgtcga tatctttaac gaacaaaaag agagattcga tttttggatc gggtcgcatt
    10261 cttttacaat atatatccct caaacgctgg gtgaatcacc acgccaattc tatccttatc
    10321 aagcttactt tggttcacac actttacagg attggtttgt cagcgataaa gatgaatacc
    10381 tgtcgcaaat tggcatcgac aagtatattg aaaaattagc tgtactggga aaaacaacaa
    10441 acacgaaaga acgctcggat atttatgcag aatttttttc aaagcgaggc cgggaagcat
    10501 ttttttgcgc ccaccttaat gaaaaaagac aaccattacg agtaaagttc aaaataactg
    10561 aaattaaccc tgaattggca ttaaagaatt tacaggaaac gcaagaattt attgataccc
    10621 atccaggaga aaatccatca gataaagtag aaaattatag aaatagagcg aaattggcaa
    10681 tgaccgaaca cctggagagc ctattggaca taaagcctga atcgagcccc tctttatttt
    10741 ggaaaaatgt cgataagaaa gaacaagaaa tgactgacag tagcaggtat aaaaagggac
    10801 caagctagta aatgaaacaa taaaaatcaa ttaaattata aattgttcta aaattgtatt
    10861 aatgggtgtt tgaaaataac gaaggagttt gatttatgat gagtcgcgaa aaagcagctg
    10921 gaactttttt taaagattac cagaaaaaaa atgtaatgag attgttacaa gactctctcg
    10981 aaaaaattat caacgaatgg ctgaaaactg atgatgaaag ccataccaaa ctgaaaagtc
    11041 ttcaagaact ttcagagatg gatattaatg ctacctcatt tgctgaaccc tcaccacttc
    11101 ctgattttgt tacgcgtctc tggctggatc cacataaagc gcttgatgcc atggacaaaa
    11161 acatttcaaa aaacgaaatc agaaagctga ttaaagaaac ggcaaaggaa atagaacttg
    11221 tctttactca tcaaaaagca cccgactact cttaattgaa atgaggatgg tgctggtata
    11281 ttaattcttc attcattttg gccatatcat tggccaaatg atgtcttatt ggataaaaac
    11341 atacacagaa aaacaattcg aaaattgact catatctaaa aacccttcta attaatttgt
    11401 tatgttaaat taagtctatg gaaccgggcc tccagaggtt cgttcttttt caccacaaat
    11461 tactttaaat ataaggggtt aaaagataat gtcatgctat attttggatt taatcgaaac
    11521 attaaaaaat gattttgtaa aaaaaaatac ccctacaaga tttgacagcg tcaagtctta
    11581 tggagagtta ttggaaaaat caaaaattaa tcccgcgatt gcagagtata tcacaggcac
    11641 ttcatccggg gttatgatgg ttgattccta tgaaaaacta ttagaactct ctaaaattaa
    11701 ctgtgctgtt gctgataata tgacacaaac tcaattgggt ctggatttaa ttgatagcta
    11761 tgaaaaattt ctggaaataa caaagataaa tgctaaagtt acttttaatt ttcaaatatt
    11821 aaattatcaa agatatgcca aactggtcga ctcatatcaa caatcttcac aagcaaaatc
    11881 tctttctggc ctaaatacct cggcaaattc ccaacttttt tttaagaaac gcgattctac
    11941 tggacaagac cgagtttcta ttaccacagg acaagcggga gtagctcatg atacagttgg
    12001 attggggctg gaatggaacg aagctatcca gaataccatt agcgagaaca tgatgaacca
    12061 gaaactttaa cagcaaaccc taatagctat tctagaattt aaatttaaaa aggccataat
    12121 cca
//
LOCUS       DASLOE010000088        11911 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.089, whole genome shotgun sequence.
ACCESSION   DASLOE010000088 DASLOE010000000
VERSION     DASLOE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11911
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.089"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(272..511)
                     /locus_tag="U6309_001857"
     CDS             complement(272..511)
                     /locus_tag="U6309_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505296.1"
                     /translation="MNKIFNLASIACFLFFSLIAFAADIPGLDDDTDKLNMEECVGEN
                     YQQCLDDICLTSDEIDCNSNCQSLAEDKCEQQAVD"
     gene            630..1925
                     /locus_tag="U6309_001858"
     CDS             630..1925
                     /locus_tag="U6309_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN5505297.1"
                     /translation="MRSKRILLTNFTRAIYSESCCIRPDTEKELIHYIAQNQPKNMLT
                     RGAGLSYSDSCLNQDGLVIDTSRFNHFIDFDVETGIVVCQGGTPFKDLFLLHDEFIPP
                     VLPGTAFATVAGGIAHDVHGKNNHSAGSFGHHISWFDLLIGDQIMHCSREKNSDLFFA
                     TIAGLGLTGIITQVAIRLKKAPRHLTIKNKPFPSIQELIAEMATYGLQQDYQVAWLDL
                     LYSEPRAILSTANYCESPNYPDEKLKIYSLPKLPFGLIKKWNMKVFNQYFYSRQKENE
                     MMSLTQYNNPLDKISHWNRLYGPRGLIQFQAVFDQDKAGQIINHILEIIRAHSATPTL
                     TVLKVFTQPGLGLLSFCRPGFTLAIDFINNPHAKKAISAMNQFIAENDGCVYLAKDLL
                     LNPEQYFTMYKNQSKFAQILTKYRCPMRSDLATRLGIIK"
     gene            1922..2644
                     /locus_tag="U6309_001859"
     CDS             1922..2644
                     /locus_tag="U6309_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN5505298.1"
                     /translation="MMQRKWVVLGATSVIAEQFAHLAAQAGNHLCLVARNSEQLALIS
                     KDIQLRYKIPCDAITINLSEPNEQLLTILETRERELDLFIAHCDFTDNNHLNPQSIKH
                     LIDTNISSTAILINTYLQSQQKKHRIVYLSSVASCRGRAKNSLYGASKAAIEIFLEGL
                     QQKATPHQHILIVRLGYIDTKQTYGLPGVFYAAQPHDCAKACWEKINHNKRLFYYPSF
                     WRVVMAIITKLPFFIYKRMGKI"
     gene            2763..3671
                     /locus_tag="U6309_001860"
     CDS             2763..3671
                     /locus_tag="U6309_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN5505299.1"
                     /translation="MNIADLQSFLAVVELHSISLAAKKLHITQPALSKRIRKLESEWN
                     TQLFISSGLRTELTDKGKQLLPYVRQMVYLSHELISSTGTDKKKPQLLLNIGTTVYIA
                     QTIIPPLIIHMNTLDLNYFINTKLIADKDVAHGLSEGAYDLIISPFMSNSAEIKSVPL
                     WREKLIPIVGLSHPLAKIKEKLSFAELAEFDAVLMEKNFIIRRIIDKEAEKQKLTLKI
                     RAEANIIYNNIALVEQGMAWSMINERLLNPNLKPLELSNYALSIDFYCHFLQKRTDER
                     LIRIFIDNLVNWVNTSSELSSFSLVN"
     gene            complement(3750..4946)
                     /gene="mavB"
                     /locus_tag="U6309_001861"
     CDS             complement(3750..4946)
                     /gene="mavB"
                     /locus_tag="U6309_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavB"
                     /protein_id="HEN5505300.1"
                     /translation="MPPPYSTHVIVNVFRCVAQLVSQNPELLNWPGVFRLAGSREESE
                     KLLEQVINDNFSVGNLACYILEDGQINNLHLNNVLGMLPLVLKESVLLKTEDPLLKLF
                     MTELKNLLSSEKNNVENTDAIVQLLSKFIDSLLLSKILDHQRVGEILYHYCYLMHTAG
                     VFHETNLMTWENLAIIMAPHFTNEFGLYPAEDLLGLIQFTNQLKPILECFIAHPDSGI
                     PFKERHADKLEHLANTRHTIVEKLTHMGSESRRIVVAPMKSLMLQASMLRAQIDAVET
                     QLKDSSLKKKDKKELNRQLEKLTEELEKLNIQISELTSKIKKMNHGHAKLKEEIRVIS
                     RSEDAVSTHATQQSGASSSSSNSVRIQLGIFGNNSSSSAYLIEEQEEGEVTENEYSGL
                     DSAKLT"
     gene            complement(5183..5380)
                     /locus_tag="U6309_001862"
     CDS             complement(5183..5380)
                     /locus_tag="U6309_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505301.1"
                     /translation="MNDLLNLIAVIVVFGVGLWLINVFIPMPGAIKSLLNVLVLIILI
                     IYILQFFGVIKTILPMIKILK"
     gene            complement(5471..8053)
                     /gene="alaS"
                     /locus_tag="U6309_001863"
     CDS             complement(5471..8053)
                     /gene="alaS"
                     /locus_tag="U6309_001863"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEN5505302.1"
                     /translation="MKSSEIRQAFLNYFVQRGHQIVASSSLVPSNDPTLLFTNAGMVQ
                     FKDLFLGLETRSYQRAATAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     REAIQYAWEFLTEVLHIPAERLWVTVYKEDLEAEDIWLKEMKVSPERFSRCGEKDNFW
                     SMGDTGPCGPCTEIFYDHGPEVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGHLHP
                     LPKPSVDTGMGLERLAAVIQGVHSNYEIDSFQYLIKAIAQLGQDIDLNHTSLKVIADH
                     IRSCSFLIVDGVLPSNEGRGYVLRRIIRRAVRHGNKLGLPSPFFSKLVQPLIDVMGDA
                     YPELINSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLQGQELSGEVAFKLYDTYG
                     FPIDLTADIIREQGLHIDMEAFNQLMQQQREQSQAASQFTTDYHAVSQLDHQSEFHGY
                     EKESMEAKIIGLLQEGNEVKSINKGAKGAVILDHTPFYAESGGQVGDKGLLIGKEFTF
                     QVDDTQKVGQAVVHYGKVIKGELTLDLLIHAQVDNIRRDAIRLNHTATHLLHAALKKI
                     VGQHVQQRGSLVDAERARFDFSHFEALTPQQIQQIEEVVNAQIRANNEVITQVMDIES
                     AKQSGAVALFGEKYSDAVRVLSMGDFSKELCGGTHARRTGDIGLFKIVAEYGIASGIR
                     RIEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEKVSQLLLDNKNQEKMIAKLLS
                     EKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMDQLKSRIDSAVIILFTIEQ
                     NKMNVIAGVSKNIIGKAPSAAQLVRHLCGKGGGRDDMAQGGGGVPEDLNSKIKEIKEM
                     IEKI"
     gene            complement(8071..8523)
                     /gene="recX"
                     /locus_tag="U6309_001864"
     CDS             complement(8071..8523)
                     /gene="recX"
                     /locus_tag="U6309_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEN5505303.1"
                     /translation="MTKAFDSALRLLTRREYSAMELCDKLKQKGFSTNDVQNALYECQ
                     RLGYQSDVRFVENYIRVRIHQGYGPLKIRQELKNKGIDPDLIQSVLHQEKDNWVNYAL
                     RAWEKKFKRQDDFSYSEIQKQQRFLLYRGFDRDVISKVFKEVKSSYLI"
     gene            complement(8516..9562)
                     /gene="recA"
                     /locus_tag="U6309_001865"
     CDS             complement(8516..9562)
                     /gene="recA"
                     /locus_tag="U6309_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEN5505304.1"
                     /translation="MEENKQKALSAALSQIERQFGKGSVMRMGDSTVSRDIEAISTGS
                     LGLDIALGIGGLPKGRIVEIYGPESSGKTTLTLQVIAECQKMGGTAAFIDAEHALDPS
                     YAQKLGVKVDELLVSQPDTGEQALEITDMLVRSAAVDVVIIDSVAALTPKAEIEGEMG
                     DSHVGLQARLMSQALRKLTANIKRSNTLVIFINQIRMKIGVMFGSPETTTGGNALKFY
                     ASVRLDIRRIGSIKKGEEILGSETRVKVVKNKVAPPFKMTEFDILYNEGISRESEIIN
                     LGVQLNLIEKSGAWYSYKQEKIGQGKENVRLYLKENPQVAAELEQQIRTELLEKKLSV
                     LASSSEDLFETIDD"
     gene            complement(9865..10800)
                     /locus_tag="U6309_001866"
     CDS             complement(9865..10800)
                     /locus_tag="U6309_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1803 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505305.1"
                     /translation="MSQYTRLFEFLCDLIDIQIKKYVELAKLKVGEDAIINAALIYEE
                     VNELLGFAEKLLKEIETNSKSDSMDMTIFDRVFEQVKFYVEQEYLRSYAEWLLIENNS
                     KDTVYNRTKEQLLRLSEIKSFDDKGFSQPSTEIQQQCQHDSVQIVYSILNLAREVRKN
                     PDMGFDDSIPHNKLNVLRRNATTRYVEFGNEIDKLHQHSEEFLQQSSLGVFDAELAYE
                     SALSKAKDHDLKLNTLFGRYKENKETASVPQSLSLVNKVDEENIMTEKKPSTEVSDNK
                     TPFFSTSNLLFFGGIVLAAVIFVPRLLTHLKVNKL"
     gene            complement(11092..11586)
                     /locus_tag="U6309_001867"
     CDS             complement(11092..11586)
                     /locus_tag="U6309_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEN5505306.1"
                     /translation="MSHFSILVEEIAAYLKKAKWQLVTAESCTGGLIAACLTEIPGSS
                     NWFERGFVTYSNLAKEEMLAVAHELIQTHGAVSEEVALAMAAGALQHSMGNIAVSVTG
                     IAGPEGGSIEKPVGTVCFAWAVHHQFQQTSRQQFTGSRQEIRLSACQYALNGVLSTLK
                     KVCG"
     gene            complement(11664..11861)
                     /locus_tag="U6309_001868"
     CDS             complement(11664..11861)
                     /locus_tag="U6309_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505307.1"
                     /translation="MNRLAMIAAASCLALFLTGCGEGSKPADTKTDQQQQQQQPTDQQ
                     QQGGENTGNNQAQPSTEGQGQ"
BASE COUNT         3731 a         2410 c         1896 g         3874 t
ORIGIN      
        1 aacccgtgat taactcattt aattcgactc tgtcaatgtt ttggcataaa aaatcatacg
       61 gtttatattg ataggactta cgaaaaactc aaggtcgagg taaaaaatgt tttggtgagg
      121 gagtttagat aaactaacta agtgaattaa aaactttttt taacatagag attgggattt
      181 tcgcaagtct tgtgcacaat atctgaggtt gtgagtattc cttaatggta gtgtaatcac
      241 ttcaaaaata aatgtttaga aaatctccca cttagtctac agcttgttgc tcgcatttat
      301 cttctgctaa actttgacaa ttggagttgc aatctatttc atcagaggtt aaacaaatat
      361 catcaaggca ttgctgataa ttttccccaa cacattcttc catgtttaat ttatcagtat
      421 catcatccag accaggaata tccgcggcaa atgctatcag ggaaaaaaac aaaaagcagg
      481 ctatgctggc aagattgaat attttgttca ttagaatacc ttccattgat atttgattta
      541 attatagaca tcctatagcc tttgttcctg tttccataat aaaaaaatca tgcgacactc
      601 actcatctgt ctatttaaaa atttctgata tgcgcagcaa aagaatttta ttaactaatt
      661 ttacccgcgc aatttatagt gaatcctgtt gtataaggcc agacacagaa aaagaactta
      721 ttcactatat tgcacaaaat cagcctaaaa acatgctaac caggggtgca ggattgagtt
      781 acagtgatag ctgtctaaat caagacggcc tggtaattga tactagtcgc tttaatcatt
      841 tcatcgattt tgatgtcgaa acaggcatcg tagtatgtca gggtggaaca ccatttaagg
      901 atctgttttt acttcatgat gaattcattc ccccagttct cccaggcaca gcatttgcca
      961 ctgtagcagg tggaatagcc catgatgttc atggtaaaaa taaccattca gcgggaagtt
     1021 ttggacatca tatttcttgg tttgatttat tgataggcga tcaaatcatg cattgtagcc
     1081 gcgaaaaaaa tagtgatcta ttttttgcaa ctatagcagg tttaggttta acaggcatta
     1141 ttacacaagt cgctattcgt cttaaaaaag caccacgcca tctgacaata aaaaataaac
     1201 catttccatc tatccaggaa ttaattgcag aaatggcgac ttatggtctt cagcaagatt
     1261 accaagtagc ctggttagac ttgttatact cagaacccag agccatttta tcaacagcaa
     1321 attattgtga gtcgccaaac tatcctgatg aaaaattaaa aatttatagc ctgcctaaat
     1381 taccatttgg acttatcaaa aaatggaaca tgaaagtatt taatcaatat ttttactcca
     1441 gacaaaaaga aaatgaaatg atgagtctca cccaatacaa taaccctctg gataaaatat
     1501 cacattggaa tcgactctac ggccctagag gacttataca gtttcaagcc gtatttgatc
     1561 aagataaggc cgggcaaatt attaatcaca tattggagat tattagggct catagtgcta
     1621 ctccaaccct cactgtcctt aaagtattta cacagcctgg attaggtttg ctttcgtttt
     1681 gtcggcctgg atttactctg gctattgatt ttattaataa tcctcacgcc aaaaaagcaa
     1741 taagcgcgat gaatcaattc attgcagaaa acgatgggtg tgtttatttg gctaaggatt
     1801 tattactaaa tccagaacaa tattttacaa tgtacaaaaa tcaatcgaaa tttgctcaaa
     1861 tacttacaaa atatcgatgc cccatgcgtt cagatcttgc gacacgatta ggaatcataa
     1921 aatgatgcag aggaaatggg ttgttttagg agctacttca gttattgccg aacaattcgc
     1981 acatcttgct gcgcaagccg gtaaccattt atgtttggtt gcacgcaatt ccgaacaatt
     2041 agccttaatc tcaaaagata ttcaattgcg atataaaatc ccttgtgacg cgataactat
     2101 caatttatcc gagcctaacg agcaattgct taccatttta gaaaccagag aaagagaatt
     2161 ggatttattt attgcgcatt gtgattttac cgataataac catttaaatc cgcaatcgat
     2221 taaacattta attgatacca atatctcatc tacagcgatc ctgattaata cctatttaca
     2281 atcacaacaa aaaaaacatc gtatcgttta tttaagctct gttgcttcct gccgcggccg
     2341 tgccaaaaat agtctttatg gtgccagtaa agcagcaatt gaaatatttc tcgaaggcct
     2401 gcaacaaaaa gcaactccac atcaacatat cttaatcgtt cgccttggat acattgatac
     2461 aaaacaaacg tatggactac ccggagtttt ttacgcagct caacctcatg actgtgcaaa
     2521 ggcatgttgg gaaaaaataa atcataataa aagattattt tactatcctt ctttctggag
     2581 agtggtcatg gctattatta caaaactccc ttttttcatt tataaaagaa tgggaaaaat
     2641 ataagtatta ttatcgcaat gattcgtttt tatacgttat aacgcccggt tataggcttt
     2701 tgtataatga taaaaactat cctagtatta atttaagtaa tattatgatt ggttttagaa
     2761 taatgaatat tgccgatttg caatcttttc ttgcagttgt agaactccac tctatctctt
     2821 tagctgccaa aaaattgcat attactcagc ctgctttaag taaaagaatt agaaaacttg
     2881 agtcggagtg gaatacccaa ctttttattt cttcaggatt aaggactgag ctaactgata
     2941 aaggaaaaca attattgcct tatgttcggc agatggttta tctgagccat gagttaatta
     3001 gcagtactgg tacagataaa aaaaagcctc agttactgct aaatattggt accacagtat
     3061 atattgctca aactattatt ccccccctta taatccatat gaacacactg gacttgaact
     3121 atttcattaa tacgaaactc attgctgaca aagatgtagc tcacggttta agtgaaggtg
     3181 cttatgatct gattatctcc ccgttcatga gtaattctgc tgaaattaag tctgtacctt
     3241 tgtggcgaga aaagctcatc ccaattgtcg ggttatcaca tcctctggca aaaattaaag
     3301 aaaaactatc ctttgctgaa ttagcagaat ttgatgcggt gcttatggaa aaaaacttta
     3361 taatacgccg aattattgat aaagaagctg aaaaacaaaa gttgactctc aaaataagag
     3421 ctgaagccaa tatcatttat aataacatag ctcttgtcga acaaggtatg gcttggagta
     3481 tgattaatga acgcttattg aatcctaatt taaaacctct cgaactatct aattatgccc
     3541 tcagcattga tttttattgt cattttcttc agaaacgtac agatgaacga ttgattcgta
     3601 tttttattga taatctggtc aattgggtta acacgtcaag tgaattaagc tctttttctc
     3661 ttgttaatta gataaaccct ttattgccag gttgcaagaa acctctcagg ctgcacttag
     3721 cccaagagat atcaattaaa actattgatt taagtcagct ttgcactatc taagccactg
     3781 tattcatttt cagtaacttc gccttcttcc tgttcctcta tcaaataggc agatgaagaa
     3841 ctattattac caaaaattcc taactgaatt ctgactgagt tactgctgct tgatgatgct
     3901 cctgattgtt gggtagcatg agtacttaca gcatcctcag agcgcgatat tactctaatt
     3961 tcttctttta gcttcgcatg gccatggttc atttttttaa ttttgcttgt taattcagaa
     4021 atttgtatat tcagtttttc taattcttct gttaattttt ctaactgtct atttaattcc
     4081 tttttatcct tctttttaag agaggaatcc ttcaattgag tttctacagc atcaatctgg
     4141 gctctcaaca tagatgcttg cagcatcaaa cttttcatag gagccacaac tattcttctg
     4201 gattcacttc ccatatgcgt taatttttca acgatagtat gacgagtatt agccaagtgc
     4261 tctaatttat cggcatgcct ttctttaaaa gggattccgc tatcaggatg cgcaataaaa
     4321 cattccagaa tgggttttaa ctgattagta aattgtatta atcccaacaa atcttctgca
     4381 gggtataaac caaattcatt agtaaaatga ggagccataa taatagcaag attttcccat
     4441 gtcattagat tggtttcatg aaaaacccct gctgtatgca ttaggtaaca atagtggtaa
     4501 agaatttctc caacacgttg atgatcaagt atttttgaaa gcaataaaga gtcaataaat
     4561 ttactcagca actgtactat tgcgtcggta ttttcaacat tattcttctc agaactcaat
     4621 agatttttta attctgtcat aaaaagcttg agcaatgggt cttcagtttt caacaagaca
     4681 ctttctttaa gaactaaagg caacattcct aaaacattat tcagatgtaa attatttatc
     4741 tggccatctt ctaaaatata acacgctaaa ttccccacac taaaattgtc attaataact
     4801 tgctcaagta acttttctga ctcttctctt gatccagcaa gtcgaaatac tcctggccaa
     4861 tttaatagct ctgggttttg tgacactaat tgggcaacac atcgaaatac attgacaatc
     4921 acatgagtac tatatggtgg tggcataaca aattccaaga ttaaattttg aacaaaatat
     4981 catgggatca aaactaaatc aattattgtt aatgaaatac gaacctgaat ttttccagcc
     5041 acattaatgt cttttggatt aattgaattc atctcaagtg ttacttgcga tgaaacttgt
     5101 gttccagaat aaaatcatta ttgtctaatc aggaacggcc tattctatta ggccgttcct
     5161 gattaatttg aggaagcaaa aatcacttta aaattttaat cattggcaat attgttttta
     5221 ttaccccaaa aaattgcaaa atataaatga ttaaaataat taataccaat acatttaaca
     5281 gacttttaat agccccgggc ataggtataa aaacattaat taaccatagg cctacaccaa
     5341 aaaccactat cactgcgatt aaattcagca agtcattcat catagtgctc attagtaaac
     5401 cagtgtctat attatagcaa atccaaaaac aacttgcttt tctgcagtcg aaaagaaaaa
     5461 tctggaaata ttaaattttt tcaatcattt cctttatttc tttgatctta ctgttcaaat
     5521 cttcaggaac acccccacca ccttgagcca tatcatctcg accacctcct ttaccacaaa
     5581 gatgtcttac taattgagca gcacttggtg ctttgccaat aatattctta ctcaccccgg
     5641 caatgacatt cattttattc tgttcaatag taaataatat gatcaccgct gaatcaattc
     5701 ttgattttaa ttgatccatg gtatggcgca tggtttggct atccataccc tctaactgct
     5761 taatcaataa attaataccc ttgatctcct cgatctcacc aagaatatct gctccagatt
     5821 tttgtgcttt ttcacttaaa agttttgcaa tcattttttc ttgattttta ttatcaagca
     5881 ataattgaga aactttttcc tgtagactat taggtgttgt tttaagggta gctgccagat
     5941 tattcataaa tcccagctgt tcattgaccc aggctaatgc ataacgaccc gtaaccatct
     6001 caatccgcct gataccgctg gcaataccat attctgctac gattttgaaa agacctatat
     6061 caccagtacg tcgtgcatga gttcctccac aaagctcctt ggaaaaatca cccatggata
     6121 atacgcgtac tgcgtcggag tatttttccc cgaataaagc aacagcacca ctttgcttgg
     6181 ctgattcaat gtccattact tgagttatta cttcattatt agctcggatc tgtgcattaa
     6241 ctacctcttc aatttgctga atttgttgag gagtcaatgc ctcgaaatga gaaaagtcaa
     6301 aacgtgccct ttcagcatcc accagggatc ctcgttgttg aacatgttgc cctacaattt
     6361 ttttaagagc agcatgtaac aaatgagttg cggtatgatt caatcgaatt gcatcccgtc
     6421 ttatgttatc tacttgcgca tggattaata aatctaaagt gagctcacct ttaattactt
     6481 ttccataatg tacgacagct tgccctactt tttgtgtatc atcgacttga aaagtaaatt
     6541 cttttcctat gagtaatcct ttatctccta cctgaccacc actttcagca taaaatgggg
     6601 tatgatcaag aattacagcc ccttttgctc ccttattaat agatttcact tcattacctt
     6661 cttgaagtaa tccaataatc tttgcttcca tggattcttt ttcataccca tgaaattcag
     6721 actgatgatc caattgagac acggcgtgat aatctgtagt aaactgactg gcggcttgag
     6781 actgttctct ttgttgttgc atcaattgat taaatgcttc catatcaata tgtaaacctt
     6841 gttcacgaat gatgtctgct gtcaaatcga tagggaaacc ataagtatca tacaacttga
     6901 atgccacttc tcctgatagt tcctggcctt gcaaattttt aatatgatct tgtaataaac
     6961 gcaatccttg ttccaaagta cgggtaaact gattttcttc ttgttgcaag atcctttcaa
     7021 tatgcgcttt cgaattgatt agctcgggat aagcatcccc cataacatca atcaaaggct
     7081 gaacaagttt ggaaaaaaat gggctgggta agcctaattt attcccatgt ctgactgctc
     7141 tgcggataat ccttcttaat acatatcctc tgccttcatt actaggcaat accccatcaa
     7201 ctatcaaaaa agagcaggaa cgaatatgat ctgcaatcac ttttaaggaa gtatgattta
     7261 aatcaatatc ctggcccaat tgtgctattg ctttaatcaa atactgaaaa ctatcaattt
     7321 cataattact atggacacct tgaataactg cggctaatcg ttccaatccc ataccagtat
     7381 ccacagaagg ctttggcaag gggtgcaaat gtccctctct gtccctgtta aactgcatga
     7441 ataccaggtt ccatatttct atataacgat ctccatcttc atctggactg cccgggggac
     7501 cacctgctac ttcaggacca tgatcataaa aaatttctgt gcaaggacca catggacccg
     7561 tatcccccat tgaccagaaa ttatcttttt ccccacatcg agagaatcgc tcgggagata
     7621 ccttcatttc ctttagccat atgtcttctg cttccaaatc ctctttatag acagtaaccc
     7681 atagccgttc tgccggtatg tgtaaaactt ctgttaggaa ttcccaagca tattgaatag
     7741 cttcgcgttt aaaataatca ccaaaactaa aattacccaa catttcaaaa aatgtatgat
     7801 gtctcgccgt ataaccaaca ttttccagat cattatgttt accacctgca cgcacacagc
     7861 gctgtgctgt agcagcacgc tgatatgaac gagtttcaag acctaaaaaa agatccttga
     7921 attgcaccat acctgcattg gtaaatagta aagtgggatc atttgaaggg accaatgagc
     7981 tagaagctac tatttgatga ccgcgttgaa caaaatagtt aaggaatgct tgtctaattt
     8041 cagaactttt cattttttaa tatttaccag ttaaattaag taagaagatt taacctcttt
     8101 aaataccttt gaaataacat ccctatcaaa ccctcgatac agcaaaaacc gttgctgttt
     8161 ttgtatttca ctataagaga aatcatcctg tctcttaaat tttttctccc atgcccgcaa
     8221 agcataattg acccaattgt ctttttcttg atgcagcaca ctttgaatga gatctggatc
     8281 tattcccttg ttttttaatt cctgcctgat ctttaaaggg ccatagcctt gatgaattcg
     8341 tactctgata taattttcta caaaacgcac atcgctttga tacccaagac gttggcattc
     8401 atacaaagca ttttgaacat cattggtgct aaatcctttc tgcttcagtt tatcacataa
     8461 ctccatagcg ctatactcgc gcctggttaa cagacgcaag gcgctatcga atgctttagt
     8521 catctatagt ctcaaaaaga tcttctgatg aactagccaa cacagataac tttttttcca
     8581 agagctcagt acggatttgt tgttcaagct ctgcagcgac ttggggattt tctttcaaat
     8641 ataatctgac attttctttg ccctgcccaa ttttttcctg tttgtagcta taccaggcac
     8701 ctgatttctc aattaaattc aattgaacac ccagattaat aatttcactc tcacgagata
     8761 taccttcgtt atataaaata tcaaactcag tcattttaaa cggtggcgct accttatttt
     8821 taactacttt aactcgggtt tcactgccta atatttcttc acccttttta attgaaccaa
     8881 tacggcgaat atccaaacga actgaagcat aaaacttcaa agcattgccc cctgtagtag
     8941 tttcagggct accaaacatc acaccaattt tcatacgtat ttgattaata aaaataacca
     9001 gtgtgttcga acgtttaatg ttagcggtca atttacgcag ggcttgcgac atcaatcttg
     9061 cctgtaaacc aacatgggaa tcgcccattt ctccctcaat ttctgctttg ggagtcaagg
     9121 cagcaaccga gtcaattatt acgacatcaa ctgctgcaga acgcaccagc atatcagtaa
     9181 tttccagagc ttgctcgcct gtatccggct gagaaaccaa cagctcatcc accttcacac
     9241 caagtttctg agcatagctt ggatccagag catgttccgc atcgataaat gctgctgtac
     9301 cacccatttt ttgacattca gcgatcactt gaagagtgag cgtggtttta ccagaagact
     9361 cagggccata aatttcaaca atacggcctt ttggcaaacc gcctatccct aaggcaatat
     9421 caagacctaa tgaacctgta gagattgctt caatatcccg tgataccgtg ctatctccca
     9481 tacgcattac agaccctttg ccaaattggc gttcaatttg cgaaagcgcc gctgatagtg
     9541 ctttttgttt attttcttcc ataatttatt ccagattata atgtgtgcaa gaacaaaatt
     9601 atcacacaga tgtgagcaag cagcaaacag taatcttagt gtattactaa ataaaagtag
     9661 catacgatag ctgatgatta gtcttttatt ataagctacg tggaataacc atttgttcaa
     9721 aaatgaaggt gttgtcagtt tgaacttcaa accttgattt ttagcttggt aacggggcaa
     9781 tataaaaata tatagacaat ccgacataaa aaccaaggtt ttacaatcaa aataacgaaa
     9841 atgttaacta ctcataatgc tgtgtcataa tttattgacc ttgagatgag taagcaatct
     9901 gggaacaaaa ataacagcgg ctaaaactat accaccgaaa aataataaat tgcttgttga
     9961 aaagaaaggg gtcttattat cagacacttc agttgaaggt tttttttctg tcataatatt
    10021 ttcctcatct accttattga ccaaagagag agattgagga acagaagcgg tttccttatt
    10081 ttctttataa cgaccaaaca aagtatttaa tttaagatca tggtcttttg cctttgatag
    10141 tgccgattca taggctaatt cggcgtcgaa aactccaaga ctcgactgtt gcaaaaattc
    10201 ttcactatgc tgatgcaatt tgtctatttc atttccgaat tcaacatatc tggtagttgc
    10261 atttcttctt aatacattga gcttgttatg tgggatgcta tcatcaaacc ccatatccgg
    10321 attttttctt acctctcttg ccaaatttaa aatggaataa actatctgaa cactatcatg
    10381 ttgacattgc tgttgaattt cagtgcttgg ttgtgaaaaa cctttatcat caaaactctt
    10441 aatttctgaa agcctgagta attgctcttt tgttctattg tagaccgtgt ctttagagtt
    10501 attttcaata agcagccatt ctgcatagga tctcagatat tcttgctcca cataaaattt
    10561 tacttgctca aaaacacgat caaaaatggt catatccatt gagtcggatt ttgaattggt
    10621 ttctatttcc tttaataact tctccgcgaa tcctaataat tcattcacct cttcgtatat
    10681 gagagcggcg tttattatcg catcttcacc tactttaagt tttgcgagct caacatattt
    10741 tttaatctgg atatcaatca gatcacataa aaactcaaac aaccttgtat attgactcat
    10801 attttttcct attttttaat caaataaatt attatacttc ttgtgcaact tttattattc
    10861 agtttaaaac aagaaacaca ctttcgagga tccgtagatt ttcattgtat aaatccactc
    10921 tacgaaatat ttgcgttaca gctataaaca aaagacactg tttatgcaag agatttattg
    10981 taatttttat tagctgaatg gtaaaaacaa gaatttgaga ctaaataata gaattgaaaa
    11041 aatatagact attgatgttt tattaaacta tgtttttaaa aaaccattcg gttagccaca
    11101 gactttttta agagtagaaa gaactccatt taaagcatat tgacaggcag acaaacgtat
    11161 ttcttgccga cttccagtga attgttgtct tgatgtttgt tgaaattgat ggtgaactgc
    11221 ccaggcaaag catacagtac ctactggttt ttcaatactg cctccttctg gacctgcgat
    11281 accagtaact gatacagcga tattacccat actatgttgt aacgcaccag cagccatagc
    11341 caaagccact tcttcactga ctgccccatg agtttgtatt agctcgtgag caacagcaag
    11401 catttcctct ttagctaaat tactataagt aacaaaacca cgctcaaacc aatttgagct
    11461 tccaggtatt tccgtaaggc aagcagcaat taaaccacca gtacaagatt ccgcagtcac
    11521 taattgccat ttggcctttt ttaaataggc agctatttct tcaaccagta tcgagaaatg
    11581 actcatacct ttcgcctata aaaaacccca taaaacatct atggggtttt cttgataatt
    11641 tataaataac actgaaatta atcttactgg ccttggcctt cagtagaagg ttgagcttgg
    11701 ttatttccag tattttcacc accttgttgt tgctgatcag tgggttgttg ctgctgctgt
    11761 tgttgatcag tctttgtatc tgcaggcttc gagccctcac cgcatccagt taaaaataat
    11821 gctagacaac tagcagcagc aatcatagct aaacgattca taatcactct ccctatttat
    11881 tttttgtaat atcacgttaa ttctagcaaa t
//
LOCUS       DASLOE010000089        11663 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.090, whole genome shotgun sequence.
ACCESSION   DASLOE010000089 DASLOE010000000
VERSION     DASLOE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11663
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.090"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1313
                     /locus_tag="U6309_001869"
     CDS             <1..1313
                     /locus_tag="U6309_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505308.1"
                     /translation="DELQKPYIHRVKTRLSELLNLDEKENELMRIEQSTSRSWEEGAT
                     TDLRIGPIKSKKRIEEIRAVLIALSGANAVKYEEGHNLENNAVEFIVTACDVEQLEGF
                     IPLNQEEMQNTIPVMTFIRMSIAGNRGYVRGISLNSEKLDGENYLSSFKEGELTTSTQ
                     EWFSVLELLDLLNSESELNLIQIQNDLKSLLGVKFEVYFHSSSEEFPIDLRTGSLDLK
                     ETGAQQLLRSMDSLVLLDCLKTESKELEDDQKVCIIKQVNPQELHRFSLKKGKNTNAN
                     AEMLSNIIELLKRTFPNNQVIQDLVVFDGRDGRSMSRPYFHIRTQIYHQEGTSDYKSA
                     MSILKKLQDKTSKKIIFDFRNEMVEMKDESRPIYISSIENTIKSSQFIIDVAVGLTTQ
                     QLPELQNICAEINQSRLKHHRHNLFKPQIEEQKINEQNQSLFLG"
     gene            complement(1402..3096)
                     /locus_tag="U6309_001870"
     CDS             complement(1402..3096)
                     /locus_tag="U6309_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505309.1"
                     /translation="MKIILSILIALHATSTYAGQQVLVLSGGDNPGLNHYTQYLQTKT
                     LYEHLITRFNPETVSIYFGGGNTPTTQPSPFDVHKLINTKDNQQNKEDVMFTGIIANN
                     QQANKYNVDAFFLSSKIQQINPGDNLLLFVSDHGMPHGFLEDKNTNPYSNNCIDLWHY
                     QKPFINNFTNAKNFSKACLSKNELSSLLALIPSQQVIFAMSQCYSGGFHQLSVTTQDG
                     YPVVNTKICGFTSSTDDHYASGCTADANGATYQGYERSFTEWYTGHGVIDGKKIREPA
                     KSMLVAHQNAIIEDMTVDIPLSTSDYYLLQWAKLFASKQFKSRTKHYDNNMIQSIYLN
                     YKKRLQSSNNDALYQFMHVARASEDKIVQLMPEAREFASLSLTKQKQKISSLEKQLEQ
                     EGKELQALWEGMMNLTLNVIMPSWEKAVNQSKSQPLNEKQQTFEKEFYQTIVKLNLYQ
                     HPYQFEMYYLQYLSEKQNDPDLVNYQKNRNAIIYQWARAHNNDPLANAIKNWEKLNKK
                     QLSLTEHIAEEEKKKQWLKRIFTYNQIIASWVTLLTIHDEKALIDLEGLLKCQNAS"
     gene            3421..5682
                     /gene="lem25"
                     /locus_tag="U6309_001871"
     CDS             3421..5682
                     /gene="lem25"
                     /locus_tag="U6309_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem25"
                     /protein_id="HEN5505310.1"
                     /translation="MSTSEKDVREQKVKTVTLSFLGTGQHREKVHHILTSFHNTISEV
                     NKDNPTVAMRMFDGPGSEPKSGDSKDPIPGTYIYNPKDNSKILISPVISQTITNAIQK
                     LTGNLAGEGIEHLLFEAVLYLNDIIEKNGGKLPETVNLHGFSRGADTCMRMANLLYQL
                     YPDIKVNLFLIDQVPGPGKRDDPHSYTVPPNVEHFESTLMLHEYRPGFDPQHSGRYVI
                     ADPEKTKVVVKPYYGEHNTGNRVTEDPNTNHTAILLHDDMNRFCRETGSLPSVGISPP
                     IIARVGDKKEEVRTHSELSPEKRFELLCGMKENEWGYAKLTKKYHERSILSKREDYVQ
                     DSRLFVNQEHRELFKQLYPKSFNWFFEKNHGGQTKKEEVIAELKSLSEDPRYEHFFSS
                     LAKHFQINENNIAGTLPEPSGIDRDEKRSFGQPPVRDRLSYLQHSLTSIANYYHYHCD
                     EKSSTNESVKNLLLERVKESRTKPDSEAIKHLEQTMDEVRQTLESKNEKGFLWQQINH
                     ISPNARQYCEQVKAALREHLEHNQVLSDTQKEEIRKAMDRMDNIVNDDSKDSQQKYRE
                     IRREVIELNAKATTPEDDNQLTRSHFQKAYFELSGDTQKTLNLESLSQTLNQLSKAHY
                     GETNMTDKITQRLDGYKNRNWFWNSVREVLNFFNIPIPKLHSEVKEQIADKLKERLVD
                     LKEKGMGNDVNAITRELGKAREDLIEHYKKTSKLEMGELDKIINKSMEELLVARKVTK
                     DLVHEEVSQVKLN"
     gene            complement(5779..6330)
                     /locus_tag="U6309_001872"
     CDS             complement(5779..6330)
                     /locus_tag="U6309_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505311.1"
                     /translation="MKKDFTLFTSARPKLKFVRLSECKEHLDTAAMWAKNAWGYMHLD
                     DEQFHYKQLQELCEHVYIAMYDGLPVGMLAVIEKTLSLEENKEESSEKIVEELSTPEI
                     TEMKNAYLDYVYIDERARSMGLSGIIIEKAKSLAREMNAEVMSLETLNSTLNGFYKRK
                     GGRVICESRYNDSYPTEIIRLKF"
     gene            complement(6487..6690)
                     /locus_tag="U6309_001873"
     CDS             complement(6487..6690)
                     /locus_tag="U6309_001873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505312.1"
                     /translation="MKTTLSLNEQEKLASLPNYYLGVTDTIASWLESNPFCLVNNVTL
                     LIQQPKLFTNYSIKPFTSSVRKA"
     gene            6743..7537
                     /locus_tag="U6309_001874"
     CDS             6743..7537
                     /locus_tag="U6309_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEN5505313.1"
                     /translation="MNKKLSLIALCASSLSLHAGTMGEVVSPTYPWFASIGTGYSWTE
                     KPGIVNPNPVFWDFSLQGYDSDLGDRGFYTFAVGKQIHRYIDISLSYLDHEVFNYQKF
                     QTSLPGTSGTPGFTGSSRTRYFELDNKALLVNGYLHPAQAWTRLASIDLTPFIDGGIG
                     YARNEVRNFYTVGTATVAGVAIGSTSSIGEPVNKNSFAWQASAGLNFHPTGSHLSIDA
                     GYRYYDGGQFNGPSVVYTNSDGFQTGTPWSGRLKANQIFVDFKYTV"
     gene            complement(7667..9382)
                     /locus_tag="U6309_001875"
     CDS             complement(7667..9382)
                     /locus_tag="U6309_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN5505314.1"
                     /translation="MKKTGYLLMFLTVACFSNQKVSAAIPLVELIAKPNPTQFVKTQS
                     YILLFYTLRNNTSVGFPLSYLFSSNNASLSNAGNTCGTFIGPNKTCQFTVQYQAPAIS
                     ITERLIVQVAYQGRSPVSDVVSINVDNNIACALLNTASYQTPFCQEQYQKVLQFTPGV
                     FNTTNQNVQEEQTLGGIFGLYQNTGSTEQICYISCGLRSLNGTPPNENTLFELASVTK
                     TFTGSVLGKFIINGTIVSQFDEIRNYPHPAGLSFNINEETVSFQQLATFSGGVCFSNA
                     PSVNQNSTDQAVNQMNFITDINALNPDPNSGNCSNGAPNVRPVYATPPFLPTHNFYSN
                     SSVGLLGQSLIRIDGFANVLEPDFNNWMCKNVINPLDMIRTSACLPNEAHDGTCGAAA
                     ANCAYTANWITAEYASGYHLFQNSYQLGNPFPFLPWAPAGGIRSNASDMIKFIRANLG
                     FNTGVHNPEQAQLIQGMLMAHEPNIYFPGGTEPNIGNQSPIRGGQGYAWVCMPSPPNG
                     DRICGKIGGHKNFRSFLGLNKSKNYGLIILFNTGSLTTDGSLIPATKPPGISQIGANL
                     LENVD"
     gene            complement(9403..10131)
                     /locus_tag="U6309_001876"
     CDS             complement(9403..10131)
                     /locus_tag="U6309_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEN5505315.1"
                     /translation="MDKYIGFRIASIVLYAIWSFSSYGTPIFRDHSLTLSGLIGYTDF
                     KDPVDQQLVISHYVTDLLTNSHKHAHPSVLISAKQQLNFEANKIHKIMLGPALYFQKL
                     HYSGDVWELMLPEFYNYQYDYTSNNVNFLIEGDLYFNPVSTWLIPFVTAGLGFGVAYV
                     HYDDYALPGIDPVSERHHFDSSIKGIYQLGAGLVVPVNPNWSINLRYAYIHMGKVHTT
                     PNSLGPIKFVLNNQNILIGINYSI"
     gene            complement(10364..11470)
                     /locus_tag="U6309_001877"
     CDS             complement(10364..11470)
                     /locus_tag="U6309_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505316.1"
                     /translation="MYNLTIHNLENYEKDPKIRLIPWALWENLFQHFISVYELSLMTL
                     SYKEAIHIFLPKTKNKEQLRQLLCLYYAHFDRNDKQFWCDVHKKGIKSEVICCAAAIT
                     GCSSALDTISLSLMPDEIVKMIQAENYYAFRLAAENGHLHVLNRLCELAPTEVMAMIQ
                     AENYHAFRLAAENGHLHVLNRLCELAPTEATAMIQSENYYAFRWAAVGRGHHNVINFL
                     LDCPAMLAYAEMHEFEYGEKYVNPFIARHVNRLKEMHDAFKQSNPEALFDLVTKSECL
                     QGFYMLRNLIRRNDEALLDDIRFLLSIPGIKALAPAGTIPGNENELLRLALRLGNQGA
                     CALLLSIPSVLVLTKANNYYIDETGGRLDLRAVA"
BASE COUNT         3654 a         2063 c         2333 g         3613 t
ORIGIN      
        1 tggatgaatt gcaaaagcct tatattcatc gtgtcaaaac caggttaagc gagcttttaa
       61 atctggatga aaaagaaaac gagctaatgc gaattgagca atcgacaagc cggtcctggg
      121 aagaaggtgc tacaaccgat ttaaggatag gtcctataaa atcaaaaaaa agaatagagg
      181 aaataagagc tgtactcatc gcattgtctg gcgccaacgc agtcaaatat gaggaggggc
      241 acaacttaga gaataatgct gtggaattta ttgttacggc atgtgatgtt gagcaactgg
      301 aagggtttat cccactgaat caggaagaaa tgcaaaatac tattcctgtt atgacgttta
      361 ttcgtatgtc aattgcaggg aatagggggt atgtgcgagg gatttcatta aattctgaaa
      421 aactcgatgg agagaattat cttagttcgt ttaaagaagg cgaactaacg acgtcgacac
      481 aagaatggtt ttccgttctt gaattattgg atttgttaaa tagtgagtca gaacttaatc
      541 ttattcaaat acagaacgac ttaaaatctc ttttgggtgt gaagtttgaa gtttattttc
      601 attcatccag tgaagaattc cccattgatt tgcgtacagg aagcttggat cttaaagaga
      661 caggggctca acaactgctg agatcgatgg atagcctcgt tttgcttgat tgtttaaaaa
      721 cagaaagcaa agaacttgag gatgaccaga aagtatgcat tatcaagcag gtaaatcctc
      781 aggaacttca caggttttca ctaaaaaaag gaaagaatac taatgcaaat gctgagatgc
      841 tctccaatat tatcgaactt ttaaagcgaa cttttccaaa taatcaggta atccaggatc
      901 tagttgtctt tgatggcaga gacgggagaa gtatgtcacg accctatttt catattagaa
      961 cccaaatcta tcaccaggaa ggtaccagtg attataaaag tgcgatgagc attctcaaaa
     1021 aacttcagga taaaacatca aaaaaaatca tttttgattt cagaaatgag atggttgaga
     1081 tgaaagatga atcaagacct atatacatta gcagtattga gaatactatt aagtcatctc
     1141 aatttattat agatgttgcg gtcggtttaa caacacaaca gctaccagaa ttgcaaaata
     1201 tatgtgctga aataaatcaa tccaggttaa aacatcatcg tcataatctc ttcaagccgc
     1261 aaattgagga acaaaaaatc aatgagcaaa atcaatcttt atttttaggt taaagtcaaa
     1321 ttcgacataa gagggataaa atgcttctta tgtcgctact ttaacattat gccttgttat
     1381 cttatcctgc aatatttttg ttcaactggc attttggcat tttaacaaac cttcgagatc
     1441 aattagagcc ttttcgtcat ggatagttaa tagtgtgacc caggaggcga tgatctgatt
     1501 gtaggtaaaa atccgcttta accactgttt tttcttttct tcttcagcga tgtgctcggt
     1561 taaggaaagc tgctttttgt ttagtttttc ccaatttttt attgcgttcg ccagaggatc
     1621 attattatgg gctcgtgccc attggtaaat aatcgcgttg cgattttttt gatagttcac
     1681 taaatcagga tcattctgtt tttctgatag atattgcaaa taatacattt caaactgata
     1741 agggtgttgg tataaattaa gtttaacaat agtttggtaa aattcttttt caaatgtttg
     1801 ttgcttctca ttgagaggct ggctttttga ttgattcact gctttttccc aggatggcat
     1861 gatgacgttg agtgtcagat tcatcattcc ctcccaaaga gcttgtaatt ctttgccttc
     1921 ttgctctaat tgcttttcca gcgaactgat tttttgtttt tgttttgtta aggataaaga
     1981 agcaaattca cgggcttcag gcattaactg gacgatttta tcttcagaag cacgggcaac
     2041 atgcataaat tggtaaaggg catcattatt gctggactga agtcttttct tataattcag
     2101 gtaaatggat tgaatcatat tgttgtcgta atgtttcgtg cgggacttaa actgttttga
     2161 tgcaaataat ttggcccatt ggagtaaata atagtccgaa gtacttaaag gaatgtctac
     2221 tgtcatgtct tcaatgatcg cgttttgatg ggcaaccaac atacttttag ccggttccct
     2281 gatttttttt ccatcaatga cgccatgccc tgtgtaccat tcggtaaaag aacgttcgta
     2341 tccttgataa gtagcgccgt tggcatcagc agtgcagcca ctggcatagt gatcatctgt
     2401 tgaggaagta aaaccacaaa ttttagtatt aactacagga tagccatctt gtgttgtaac
     2461 agataattga tgaaacccac ctgagtaaca ctgagacatc gcaaaaataa cctgttggct
     2521 tggtattaag gcgagcagcg acgataattc atttttggat aaacaggctt tgctaaagtt
     2581 tttggcgtta gtgaagttgt taataaatgg tttttgataa tgccagagat caatgcaatt
     2641 gttactgtag ggattggtat ttttatcctc taaaaaccca tgaggcatgc catgatcact
     2701 gacaaagaga agcaaattat caccagggtt gatttgttga attttggaag ataaaaaaaa
     2761 ggcatccaca ttgtatttat tggcttgttg attgtttgcg ataatacctg taaacatgac
     2821 gtcttctttg ttttgttgat tatcttttgt attaatgagt ttgtgcacat caaatggaga
     2881 aggttgtgtg gtcggtgtgt tacctcctcc aaaatagatg cttacggttt cgggattaaa
     2941 tcgggtgatt aaatgctcat aaagagtttt tgtttgcagg tattgggtgt agtggttaag
     3001 gcctgggtta tctcctcctg acagtaccaa aacttgctgg ccagcataag tagaagtggc
     3061 gtgcagagca attaaaatgc tgagaattat tttcatcttc attccatgaa tcgttgtttt
     3121 aattcttttc attgttgtgt caaggatagc aattcttgta ttgaactgct aacgataatt
     3181 gaaacgttac gattaaagac taagactaat tagagatagg caagatatag tcagccatca
     3241 gcactgctac tccttgtttt ttgattaccc ttctctaacc ttactccctg ttcgagttca
     3301 atacaggtgt taaataagtc tatttttaat aaagacattc attttgagta attgtggagc
     3361 atttattgtg cgtatggttt aaaattatat caaggaccat tattagttga tattcctgtt
     3421 atgagcacat ccgaaaaaga cgttcgtgaa caaaaagtaa aaacagtcac attaagtttt
     3481 ctgggtacag gacaacatcg tgaaaaggtt catcatattc tcacgagttt tcataataca
     3541 atctcggaag taaataaaga caatcctaca gtggcaatga gaatgtttga tggccctggt
     3601 tcagaaccaa aatcaggcga cagcaaggat ccaattcctg ggacttatat ttataaccca
     3661 aaagataatt ccaagatatt aattagtcct gtaatctccc aaacgattac caatgccata
     3721 caaaaactga caggaaattt ggcgggagaa ggtatagagc atttattatt tgaggcagta
     3781 ctttacctta atgacatcat tgaaaaaaat ggaggcaaac tgcctgaaac agttaatttg
     3841 catgggttta gccgaggggc tgatacttgt atgcgcatgg cgaatcttct ttatcaatta
     3901 tatcccgaca tcaaggtcaa tttatttctt attgatcagg ttcccggacc tgggaaacgg
     3961 gatgatcctc attcttatac tgttcctccg aatgtagagc attttgagtc gacattgatg
     4021 ttacatgaat acaggcctgg atttgaccct caacattctg gaaggtatgt gattgcagat
     4081 cccgaaaaaa caaaggtagt tgttaaaccg tattatggag aacacaatac cggtaatcgt
     4141 gttaccgaag atcccaacac aaatcacacc gctattttat tacatgatga tatgaatcgc
     4201 ttttgcaggg aaaccggctc actcccttct gttgggattt ctccacccat tattgccaga
     4261 gtaggagata aaaaagagga ggttagaact cattcagaat tatctccaga aaaacgcttt
     4321 gagttgttat gtggtatgaa ggaaaacgaa tggggctatg ccaaactgac caaaaaatat
     4381 catgagcgca gtattctgtc gaagcgggaa gattacgtac aagactccag attgtttgtc
     4441 aaccaggaac accgggaatt gtttaaacaa ttgtatccca aatcatttaa ttggtttttt
     4501 gaaaaaaatc atggcggtca aacaaaaaag gaagaagtca tcgctgaatt aaaatcacta
     4561 tccgaagatc caaggtatga gcattttttt tcatcattag cgaaacactt tcaaattaat
     4621 gagaataata ttgcaggaac attgccagaa ccctcgggca ttgataggga tgaaaaaaga
     4681 agcttcggac aacccccagt cagggacagg ctttcttatt tacaacattc gctgacttcc
     4741 attgcgaatt attatcacta tcattgtgat gagaaaagct caactaatga atcagttaaa
     4801 aatttgctgt tagaacgtgt aaaagagtca aggacaaaac cggatagcga agcaatcaaa
     4861 catttggaac agaccatgga cgaggtaaga cagacactcg aatccaaaaa tgaaaaaggt
     4921 ttcctctggc aacagattaa tcatatttct cccaatgctc gacaatattg tgaacaagtg
     4981 aaagcagcac tccgggaaca cctcgagcat aatcaggtac tatcagatac tcaaaaagag
     5041 gaaattcgta aggctatgga tagaatggat aatatagtca atgatgatag caaagacagt
     5101 caacaaaaat atagggaaat aagaagagag gtgattgagt taaatgccaa ggccaccaca
     5161 ccggaagatg ataatcaatt aacaagaagt catttccaaa aagcctattt tgaattatca
     5221 ggggatacac aaaaaactct gaatttagaa tctctgtctc aaacattgaa tcaactatcg
     5281 aaagcccatt atggtgaaac caatatgacg gacaaaatta ctcagcgatt ggatgggtat
     5341 aaaaatcgaa attggttttg gaactcagtc agggaagttt tgaatttttt caatattcct
     5401 attcccaaat tgcattcgga ggttaaagag caaattgctg acaagctcaa agaaagactt
     5461 gttgatctca aggaaaaggg tatgggtaat gatgtgaatg caatcaccag agagcttggt
     5521 aaagctcgcg aagatttaat tgaacattat aagaaaactt caaaactgga aatgggagaa
     5581 ctggataaga ttatcaataa atcaatggaa gaactccttg tggcacgcaa agtcaccaaa
     5641 gacttggtcc acgaggaagt tagtcaggtt aaattaaatt aagcactgtt ctgttttgca
     5701 aagttatcat aagcaaggtt ttgggtttaa caataatgac ttctctagct caaattgcag
     5761 ataaaactga tcctcttgtt aaaattttag tcttatgatt tcagtaggat aactatcgtt
     5821 atacctgctt tcacaaatta ctcttccccc ctttcttttg tagaatccat tgagggtgga
     5881 gttcagtgtt tcaagagaca ttacttcagc attcatttct cttgcaaggc tttttgcttt
     5941 ttcaataatt attccagaga gccccatgct tcgcgcacgt tcatcaatgt acacatagtc
     6001 caaataagca tttttcattt cagtgatttc tggggttgag agttcttcca caattttttc
     6061 ggaggattcc tctttgtttt cttcaagaga gagtgttttt tcaatgacag cgagcattcc
     6121 aaccggtagt ccgtcataca tcgcgatata aacatgttca cacaattcct gcagttgttt
     6181 gtaatgaaat tgttcgtcat ccagatgcat gtatccccag gcattctttg cccacattgc
     6241 tgcagtatcc agatgttcct tgcactcgga taatctgaca aattttagtt taggtcttgc
     6301 tgatgtgaat aaagtgaagt cttttttcaa acttgtacct cttgtgaata tgaattaatg
     6361 aattgaaaaa gtgctatatg atactgttta tctgtgcttt atcaacttga ttatttcata
     6421 aattcttgta gattgactta gaggagcctg cttgtataaa tacgctcaca atattgaatt
     6481 aagttatcat gcttttctaa cgctgcttgt aaagggctta attgaatagt tcgtaaaaag
     6541 tttgggctgt tgtatcaata acgtgacatt gttcaccaaa caaaagggat tactctctag
     6601 ccaagaagct atggtatcag ttaccccaag atagtagttt ggcaagcttg ctaatttttc
     6661 ttgttcattt aaacttaacg tcgttttcaa tggcacttca taacagcgtg tctgttatac
     6721 ctattccaat aaggatatta tcgtgaataa aaagttgagt ttaattgctc tgtgtgcttc
     6781 ttcgctttct ttgcatgctg gaacaatggg agaagttgtt tcacctacct atccatggtt
     6841 tgcttctata ggcactggtt attcctggac tgaaaagcca gggattgtta atcctaatcc
     6901 tgttttttgg gatttttcat tacaaggtta tgattctgat ctgggggata ggggatttta
     6961 tacctttgct gttgggaaac aaattcatcg gtatattgat attagcttaa gttatcttga
     7021 ccatgaggtt tttaactatc aaaaattcca aacatcacta cctggcactt ctggtacccc
     7081 tggttttaca gggagctcaa gaactcgcta ttttgaactt gataataaag cattattagt
     7141 aaatggctac ctacatccag cccaggcgtg gactaggctg gcgagtattg atttgacccc
     7201 tttcatagat ggtggtatcg gttatgcacg taatgaagta cgtaattttt ataccgtagg
     7261 gacagcaacg gtagctggtg ttgctattgg ttctacgtca agtattggtg agcctgttaa
     7321 caaaaacagt tttgcgtggc aagcgtctgc cgggctgaat tttcatccaa caggaagcca
     7381 tctgagtatc gatgcgggtt atcgttatta tgacggcggt caatttaatg ggccatcagt
     7441 tgtctatacc aacagcgatg gttttcagac aggaacaccc tggtcaggtc gtttgaaggc
     7501 aaaccagatt tttgtcgact ttaagtacac agtgtaatta tttaagctag taaaattgga
     7561 ttactgcatt gttgagctaa atggtttatg tggccgttca cacatagcag gtaaaggagt
     7621 gatgaacggt tgcattcctg tttcctatct atcgaagcag aatgtattaa tcaacattct
     7681 ctaataaatt agcgccaatc tgacttatac ccggtggttt tgtcgcagga atcaaacttc
     7741 catctgtcgt aagagagcct gtattaaata aaataatcaa accataattt ttacttttat
     7801 ttaagccaag aaagctgcga aaatttttat gtccgccaat ttttccacag atcctgtctc
     7861 cattaggggg agaaggcatg catacccagg catagccttg gccaccacgt atagggcttt
     7921 ggttgcctat attaggctct gtgccaccag ggaagtaaat atttggttca tgagccatca
     7981 acatgccttg aattagctgg gcttgttcag ggttatgaac cccagtgtta aagcccaaat
     8041 tagcgcgtat gaatttaatc atatcagagg cgttactacg aattccgcca gcaggtgccc
     8101 agggtaaaaa tgggaaagga ttgcccaact gatagctgtt ttgaaataga tgatatcctg
     8161 aggcgtattc agctgttatc caatttgcag tgtaagcaca gtttgcagct gctgcaccac
     8221 aagtaccatc gtgagcctca ttaggaagac aagcgcttgt tcttatcata tccagaggat
     8281 tgatgacgtt cttacacatc caattattga agtcgggttc aaggacatta gcaaaaccat
     8341 cgatacggat aagactttgt cctagtaagc ccacgctact gtttgagtag aaattatgtg
     8401 ttggtaaaaa tggcggagtg gcgtatacag gtcttacatt gggtgcacca ttactacaat
     8461 tgccgctatt gggatcagga tttagcgcat tgatatctgt tatgaaattc atttggttga
     8521 cggcttgatc tgtactgttc tgattgacac tgggagcatt ggagaaacaa actcctccag
     8581 agaaagtagc caattgttga aaactcacag tttcttcatt gatattgaag ctaagacctg
     8641 caggatgagg ataattccga atttcatcaa attgggaaac aatagtgcca ttgataataa
     8701 atttccctaa taccgacccg gtaaaagttt tggtgacgga ggctaattca aatagtgtgt
     8761 tttcattggg cggtgtacca tttaaagacc ttaatccaca acttatgtaa cagatctgtt
     8821 cagtgcttcc tgtattctga tacaggccaa atatccctcc taaagtttgt tcttcttgaa
     8881 cattttgatt tgttgtattg aatacaccgg gagtaaattg aagtactttt tggtattgtt
     8941 cttggcagaa aggagtttga taggatgccg tattgagtaa ggcacaggct atgttattat
     9001 ctacattgat agagacaaca tcgcttactg gcgaccgtcc ctgataagct acctggacta
     9061 tcaatctttc tgtgatgcta atagcaggag cttggtattg gacagtaaat tgacatgttt
     9121 tgtttggacc aataaatgtt ccacaggtgt ttccagcatt tgataggctg gcattattgc
     9181 tggaaaataa atagctaagt ggaaagccaa cacttgtatt attgcgcaaa gtataaaata
     9241 acaagatata actttgagtt tttacaaatt gagtggggtt gggctttgct atcaactcaa
     9301 ccagggggat ggctgccgat actttctggt tactaaaaca tgcaactgtt aaaaacatta
     9361 ataaataacc tgtttttttc atatttcgcc tgagttattg agttatatcg agtaattaat
     9421 ccctatcagg atattttgat tgtttaaaac aaatttaata ggtccaaggg agttaggggt
     9481 agtatggact ttgcccatat gaatataagc atagcgtaaa ttaattgacc aattagggtt
     9541 gactggtact acaaggcctg ctcccaattg ataaatccct ttgatagaag agtcaaaatg
     9601 atgtcgctca cttacaggat caatgccagg aagggcataa tcgtcatagt gaacataggc
     9661 aactccaaat cccaaaccag cagtgacaaa aggaataagc caagtagata caggattaaa
     9721 atacaggtct ccttctatta aaaaattgac attattactt gtataatcat attgataatt
     9781 atagaactca ggtaacatta attcccacac atcgccagaa taatgcagtt tttgaaaata
     9841 caatgcagga cccagcatga ttttatgaat cttattggct tcaaaattca attgttgctt
     9901 ggcagaaatt aatacgcttg ggtgagcatg tttatgggaa ttagtcagta aatcagtgac
     9961 atagtgactg attaccaact gttgatccac aggatctttg aaatccgtat aaccgataag
    10021 gcctgataga gtcaggctat gatcacgaaa tattggtgtg ccataagagg aaaaagacca
    10081 tattgcataa agaactatcg aggctatcct aaatccaatg tatttgtcca cagacatcct
    10141 tttgagtatt cacaatgatt aaaaattcat acttataatg aagcgaataa attatcatga
    10201 aataaaaaaa taggaagtaa aatgaggaga aaaaaaatgt tttttgtatt atatatcgag
    10261 catggaaatt aatgctcaat taaattcagc cgataatttt ctgttcgatc aggggtttta
    10321 taaagtatca catcagatca attgaagtga cttgaacgga atcttatgct accgctctta
    10381 agtcgagtcg ccctcctgtc tcatcaatat aataattgtt tgccttagtt aataccaata
    10441 cagatggaat agataatagt aatgcgcaag cgccttgatt acctaatcgc agagccaggc
    10501 gcagtaactc attctcattt cctggaatgg ttccagcagg agccaaagct tttataccgg
    10561 ggatgctcag caaaaaacga atatcatcca gtaaagcctc atcattacga cggattaaat
    10621 tacgtaacat ataaaagcct tgcaggcact ccgactttgt gactaaatca aagagagctt
    10681 ccgggttact ttgtttaaag gcatcatgca tttcttttaa cctgttcaca tgcctcgcaa
    10741 taaacggatt gacgtacttc tctccatatt caaattcatg catttctgca tacgcgagca
    10801 tagctgggca atcgagcaaa aaattaatga cattatgatg gcctcttcca actgctgccc
    10861 agcgaaaagc ataataattt tctgactgaa tcattgctgt ggcttcagtt ggcgccaatt
    10921 cacataaacg attcagcaca tgcaggtgtc cattttctgc agccagacga aaggcatggt
    10981 aattttctgc ctgaatcatc gccatgactt cagttggcgc caactcacat aaacgattta
    11041 gcacatgcag atggccgttt tctgcggcca gacgaaaagc atagtaattt tctgcctgaa
    11101 tcattttcac aatctcatca ggcattaaag aaagagatat tgtatctaag gcacttgagc
    11161 aacccgttat tgcagcagca caacaaataa cctcgctttt tattcctttc ttgtgaacat
    11221 cacaccagaa ttgtttgtca ttcctgtcaa aatgggcata gtatagacac aacaattgcc
    11281 tcagctgttc cttatttttt gtctttggta aaaagatatg gattgcttct ttataagata
    11341 aagtcatgag agataattca tatacactga taaaatgctg aaataaattt tcccatagag
    11401 cccatggtat taatcgtatc ttcggatctt tctcatagtt ttctagatta tgaattgtta
    11461 gattatacat agagctaaat aaataaaaaa caaactaaat aattataaat taatcacaat
    11521 tcggctttat ggtcgaaagc ggacaatcta tggttaaaag aggacaatat gaaatatttt
    11581 aaaccggaat caattaaaca gtttatttcc tgcatgttgt tatgcgttgt tgtttgtttt
    11641 tctttatttt ggataaatag tct
//
LOCUS       DASLOE010000090        11613 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.091, whole genome shotgun sequence.
ACCESSION   DASLOE010000090 DASLOE010000000
VERSION     DASLOE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11613
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.091"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1451
                     /locus_tag="U6309_001878"
     CDS             <1..1451
                     /locus_tag="U6309_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5505317.1"
                     /translation="QAFSTFSYAVQYSSLALFITKQLGIANLSANSIVGLFLSFSYIF
                     QLIGGVIGSRFISNRLLFVTTILIQNMGLFFLAQANPSSLNIGLSLFMVGSGLNVTSS
                     NSMLTQCFIPQDDRRESAFFLSYASMNVGYCIGYIACGFFDYSNQYQYLFYASILTNS
                     IALLIIIKNWAYLGDKNFPLPDLTKRNWYFIKNILAIAFITILIPFMRVCFEFANFCN
                     GLVIITCLFMFLIIFILGLKQKSSSDRKKMMAYLILAISSVLFWMIYLTGPMGVTLFV
                     KNNVDKHLFSFELATQWIKNINPVIIILGAPIMAMLINKLKAKGFHVSVPVQFSCSFV
                     FLALSFLCLIFGIICSNSYGYISVYWIIGHHILQGMSELLLGPVGYALIGRIAPPKLQ
                     GILMGTWMLVTGVAASFSQYFSNAMVQSESVDPLITNQDFLTAFKQLGLWSLLCAALL
                     YFVSGKLTRIINGIKDNKSVNESKALPMPYSN"
     gene            complement(1456..2037)
                     /locus_tag="U6309_001879"
     CDS             complement(1456..2037)
                     /locus_tag="U6309_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505318.1"
                     /translation="MLTITREKFEEIKNQLKEKQLVCNQIAQWKKTPLWLREITHQLE
                     IPPNFLNELSQIRDAIVSFEIDKNTLGLAVFYNELYKLLAPSYNVLTPLDTDLMNSHV
                     LLLQRISHYAGKVDGYIKSLSSTEVKHLDYTEFWSMLNEICSIQEVDRRKQAKEKFSQ
                     SIFLRLYQYNPKIYLDVAKSLEEPDEQSHILDF"
     gene            complement(2297..2833)
                     /locus_tag="U6309_001880"
     CDS             complement(2297..2833)
                     /locus_tag="U6309_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5505319.1"
                     /translation="MSAKQKDLERLLELKKKQEDLQVLNEKDMQERIKLERKYMDFLQ
                     MTSQQMEEELKKRGPVKEVDLKGKDIDPIIEDYKKLYSKESWYKEPETKDGKTHLTFP
                     SQEAAGNFFKDQAGKNRSFIVIDGATNKVLAYSNGDGKLYNGNGSVYQGGDFKASKED
                     FTSFKMPEREDPKMGMQL"
     gene            complement(2966..4396)
                     /locus_tag="U6309_001881"
     CDS             complement(2966..4396)
                     /locus_tag="U6309_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEN5505320.1"
                     /translation="MDLSLLKKINDFEWQIPQHGKMLVPGVIFASEQLILDMDMKVYE
                     QLSNVATLPGIVSAAYAMPDAHWGYGFPIGGVAAFDPDDGGVISAGGVGFDISCGVRL
                     LSTGLKRDELESVKVSLADALFTHIPAGVGSRSGIHLTIKQLDDMMRGGAQWAVKQGY
                     GEAADLERIEDRGCVEGAIPDFVSEQAKKRQKNEMGTLGSGNHYLEVQEVRQIYCSDT
                     ATALNLHEGDIVVSIHCGSRGLGHQIGTDYLRSMVIESQHYGIRLADRELACAPIHSK
                     LGENYLGAMRAGINCALANREIITHFMRNVFQDILPHTQIDLIYDVSHNTCKEEFHEI
                     DGKKKRLFVHRKGATRALGPGHPQLPKAVSHIGQPVIIGGSMGTGSYVLAGVKGSEHK
                     SFSSACHGAGRAMSRHQAMKKWNGKDIVDKLAQQAILIRSGSYRGVAEEAPGAYKDVH
                     SIVDAAEMSGLAKKVAKLIPIICVKG"
     gene            complement(4430..4873)
                     /locus_tag="U6309_001882"
     CDS             complement(4430..4873)
                     /locus_tag="U6309_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEN5505321.1"
                     /translation="MSKRQNPLPLNKYWEHFTHEADIGVRGIGPTLTDAFEMGALALT
                     NVVADATLIKPSLSIKIHCEAPNEEILFTDWLNAVVYNMQIHNMLFREFDVKLNRFAL
                     DAVIKGEKVDQKKHQPVVEVKGATYTELKVYKNNDIWIAQCVVDV"
     gene            complement(4843..5127)
                     /locus_tag="U6309_001883"
     CDS             complement(4843..5127)
                     /locus_tag="U6309_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEN5505322.1"
                     /translation="MGKFWSQRVTNTSNALDLEKGVFTWNDPQKIAVSLLNSALNSNR
                     RKGTPYQSAMSMLNFYINRAGSKLAAGQKEILMEAKKELRLLAKDKTRYH"
     gene            complement(5211..6764)
                     /locus_tag="U6309_001884"
     CDS             complement(5211..6764)
                     /locus_tag="U6309_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate O-acetyltransferase"
                     /protein_id="HEN5505323.1"
                     /translation="MRIFSRFSDRLVIFAFLVIIFVPGIGMFFEKQADEVRSLLNREP
                     HQLPPINIKKIGRTDFKGIENWFVDHTLFMTSLSKFWSHVVYQLGASIKPGQAILGKE
                     DWLFLGNDYAASIDQYTGRNKPTEEEILLKLSVLKQMNHLAKQNNIPFLVAIAPDKHE
                     IYPEYLPANVHKSSNKNRLDLLQEGMLARGIDFINLRQKEIEAKNTLGKQYGDLYLKG
                     DSHWNYVGAYVAYKAISDYMQQKGLQSRQLQFHFIPRETTYSDLTNFLQLTHIKSNNP
                     LPDVSNLKIDLFGRDIDGKEIKLDDFQGNPNGVILIAPYENINKAVQNKQTCLLIGDS
                     FSESLSFYFHNDFYNTVRIHSGNTSWNLSDLIQKYHPDLIVYEKVERDLLYPLVNFQT
                     TANQVSLELPKQALAARGELDKFKIGPDTISVNGWAYIPGLDAGNGEVFLKLSMGAHT
                     YLYSMNKMQKQSVNLAFKQDGKHLDLSGFNGTISRKDLPSGLYKVSLIVLNGEEMGET
                     ELLGTYTLS"
     gene            complement(6767..8188)
                     /locus_tag="U6309_001885"
     CDS             complement(6767..8188)
                     /locus_tag="U6309_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEN5505324.1"
                     /translation="MVFSSLIFLFLFLPCLNLTYLVCFKSIKWQNFVLMLFSLSFYYW
                     GEKDHIFIMLACILINYLCGLLIEDSKKPGQRKIYLITSVSASLAMLMYFKYFNFFLD
                     SLNSVFDLNVMGISKIVLPLGISFYTFHALSYVVDVYWKKVPAERNFVTFVCYVSFFP
                     QLVAGPIVRYADIRHSFYRRYITRAGVRNGILRFCFGLAKKVLIANEVAKLADYVFYL
                     PANELTFWVAWLGAIAYALQIYFDFSGYSDMAIGLGLMFGFKYKENFNFPYNALSIQE
                     FWRKWHISLSSWFRDYLYIPLGGSYKSRFRTQINLIIVFGLCGLWHGANYTFIVWGLF
                     HGFFLILERTHFGYFMKKLPKVIRHGYVLSVVTVGWVFFREDNIQSALAFLKTMFIPA
                     EFNLDSIAACINIILIFAFIVGIILALGFNSRIRDSLILSRNKNWIIAYKQSSKVLGI
                     LLLLVSILLLSTNSYNPFLYFRF"
     gene            8404..9042
                     /locus_tag="U6309_001886"
     CDS             8404..9042
                     /locus_tag="U6309_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505325.1"
                     /translation="MQDSFFSFLSSPINLLFLFVAFFLLFVTPWLWSMVNSTHNIHKL
                     LDLIGIKKKENSRVVNGLLLLPLLLITIFIEWLWPLANITLFPILASPYIKFNIIAVI
                     SVIILLILIQFDKKKSMGLWVLAYAAVLYLLYLIIIMATVVVGMNTFPASHYIFAPES
                     SMTINRTIIYGLLIAHLIFIWLIISLKFTWRLLLMILYTLALSFAYFSLIVS"
     gene            9277..10377
                     /gene="wipC"
                     /locus_tag="U6309_001887"
     CDS             9277..10377
                     /gene="wipC"
                     /locus_tag="U6309_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector WipC"
                     /protein_id="HEN5505326.1"
                     /translation="MNRMALFIIFIIHCYFSQSFAEQEKPYNELYVKQANLKQYPKEI
                     NSYPPGVEITIGDLHGNALKLLYFLIRNDVIKIDKEDYKLFVTIYQKNPNELTTKDLS
                     FFQIIVNSAEINTQHKIRFLGDDLCDRGMNDYYTLVIYKKLDQANVPFEVILSNHGNF
                     FLTAYERPEQSFNYNPYGEGENESTVQSMLNMGRLIDRGLIDKQDILEMIQYHYLKHI
                     VLPGYTHNKDKSELTIYTHAPIDLGIISTLANDLQVPFKDSNLYELTKSLDAINSKIK
                     QWILSNTFTRHYKELNEAHNQTNTPSPIKQILWNRDYSILDRHANPNNKPYGINYVHG
                     HDSMPNVFDLDNLFGKGEDFYQGPYAVHITHS"
     gene            complement(10425..>11613)
                     /locus_tag="U6309_001888"
     CDS             complement(10425..>11613)
                     /locus_tag="U6309_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEN5505327.1"
                     /translation="SFNFSTSTIPVSKAEPQGNLLYSEIPSIKMPLNEIKTLLQKEGN
                     SLQPAVIDKVITTIQCANAYQVDRNNILTIIDYSMPSNQKRLWVFDLDKKELLFHTYV
                     SHGIKSGALLTDKFSNKFDSKASSIGVYKTEQVYYGREGLSLRLVGLDTKFNDNAFNR
                     YIVMHGGWYMDEQFIKRYGRPGRSWGCPALPLPIKKQIIDTIKDNSLLVVYYPSDEWF
                     NKSKFLNCSKQKSDQVAINRLSETQAPVDDEIREDILFVDLNKNNSREEHEPIITMSA
                     DAYERIFHSQPPLTRMLRRQINNAEYIALSKEEFNKLVLQGNREGLGEIHFVIPVIIM
                     EHGYYETQMQIVNMGKIKEVQPNSDTSRITQEPAKSYRIDFESRPALNLKTTNRFIRW
                     LGL"
BASE COUNT         3594 a         2358 c         1925 g         3736 t
ORIGIN      
        1 ttcaggcatt ttcgactttt tcatacgctg ttcaatattc ttcgctcgca ctttttatta
       61 ccaaacaact tggaattgcc aatttatcag ccaatagcat agtgggcctg tttttaagtt
      121 ttagctatat ttttcaacta attggcggcg tcatcggaag ccgttttata agtaacagac
      181 tactatttgt tacaactatt cttattcaaa atatgggttt attcttttta gcgcaagcaa
      241 atccgtcttc acttaacata ggcttaagtt tgtttatggt aggttcgggt ttgaatgtta
      301 cttccagcaa cagcatgctt actcaatgtt tcataccaca ggatgatcgc agggaaagtg
      361 cattttttct cagttatgcc agcatgaatg tcggttattg cataggttat atagcctgcg
      421 gtttttttga ttactccaac cagtatcaat atttgttcta tgcaagcatc ctgactaatt
      481 ccattgcttt attgattatt atcaaaaact gggcctacct tggcgataaa aattttccat
      541 tacccgatct cactaaacga aactggtatt tcataaaaaa tattctggca atagcattca
      601 ttacaatcct tattcctttt atgcgggtat gttttgagtt tgcaaacttt tgtaatggat
      661 tggtaataat tacctgttta ttcatgtttc ttatcatctt tatcctgggg ctaaaacaaa
      721 aatcctcctc cgataggaaa aaaatgatgg catatttaat tttggcaatc agttcggttt
      781 tattttggat gatttatcta actggcccga tgggtgtaac tctttttgtc aaaaataatg
      841 tggataagca tcttttcagc tttgaattgg ctactcaatg gataaaaaat attaatcccg
      901 tgatcattat tttaggagca ccaatcatgg ccatgctcat taacaaatta aaagcaaaag
      961 gttttcatgt ctctgttcct gtgcaatttt cctgttcctt cgtgtttttg gccctgtcct
     1021 ttttatgcct tatctttgga attatttgtt caaattccta tgggtatatc agcgtatact
     1081 ggatcatagg ccatcatatc ttgcaaggga tgtctgagtt gcttcttggt ccggttggct
     1141 atgcgttaat tggaagaata gctcccccaa aactacaagg gattttaatg ggcacctgga
     1201 tgctggttac aggtgtcgca gcctcttttt cccaatattt ctccaatgcc atggtgcaat
     1261 cagaatctgt agacccccta attacaaatc aggattttct aactgcattt aagcaattgg
     1321 gactttggtc tttactctgt gctgccttgt tatattttgt ttcaggtaaa ttaactagga
     1381 taataaatgg tataaaagac aataaatcag ttaatgagag caaggctctc ccaatgcctt
     1441 actccaacta atcatttaaa aatccagaat atggctttgc tcatcgggtt cttccagact
     1501 tttagcaaca tccaggtaaa ttttcggatt gtactggtac aaacgcaaga atatgctttg
     1561 ggaaaatttt tcctttgcct gcttgcgcct gtctacctct tgaatagaac atatctcatt
     1621 aagcattgac caaaactcgg tgtaatccaa atgctttact tcagttgatg ataaactttt
     1681 tatataccca tctactttac ccgcatagtg gctaattctc tgaagcaata acacatgact
     1741 attcataaga tctgtatcaa gtggtgttaa tacattgtac gatggagcta aaagcttata
     1801 aagctcatta taaaacactg ccaaacctaa ggtattctta tcaatctcaa aggatacaat
     1861 agcatcccga atctggctta attcgtttaa aaaattgggg ggaatctcta gctgatgagt
     1921 gatttctctc aaccacagcg gagttttttt ccattgcgca atttgattgc aaactaattg
     1981 tttctctttt agctggtttt taatttcctc aaatttttct ctggtaatag ttaacatgtt
     2041 tttttctctt ataaataagc aagcgatacc caagaagtta gggtttgctt ccaaaattgg
     2101 gaataggctt tttgcatagc ctgtctcttt atgcaagaag tctaatgttt gataacgttt
     2161 atgttttttc agaccagatg atacattatt tgacacttac tgtccaaatt ggacagtaat
     2221 gtttttttgg aagaggaaga gaatgaataa tcataaaaag attatctgca tcgaatccga
     2281 tttaaaccaa agcagattaa agttgcatcc ccattttagg atcttctctt tcaggcattt
     2341 taaagctggt gaaatcttct tttgacgctt tgaagtcacc gccttggtaa acactaccat
     2401 tcccattgta tagttttcca tcgccattag agtaagctag cactttgttg gttgccccat
     2461 cgataacaat aaaagatctg ttttttccag cctgatcttt aaagaagttg cctgctgctt
     2521 cctgagacgg gaaagtcaaa tgagtttttc cgtctttcgt ttcgggttct ttataccaac
     2581 tttccttact ataaagcttt ttgtagtctt caataatagg gtcaatatct ttacccttca
     2641 aatcaacttc ttttacagga cctcttttct ttaattcttc ttccatttgc tggctggtca
     2701 tctgcaaaaa atccatatat tttctctcaa gctttattct ttcttgcata tctttctcat
     2761 tcaaaacttg caagtcttcc tgttttttct ttaattcaag taatctctca agatcttttt
     2821 gttttgcgct cattctggat gtctcctcgt acttattgag ctaattttag tctaaaaatt
     2881 tatcgaaaat tgctcttagt ttattttatt tacagattcc ttacaattat aagctgctcc
     2941 attcacctca ataaacaatg gattgttaac ctttgacaca aatgatggga attaatttag
     3001 caactttttt tgccaaaccc gacatttcag cggcatcaac aatagaatga acatctttat
     3061 aagctcctgg agcttcctca gcaaccccac ggtatgatcc gctacgaatt aaaatcgctt
     3121 gttgagccaa tttatctact atgtctttcc cgttccattt cttcatcgcc tgatgacgac
     3181 tcatagcgcg acctgctcca tggcatgccg agctaaaaga tttatgctca gaacctttga
     3241 ctcctgctaa tacataggag cctgttccca tactccctcc aataatcaca ggttgaccta
     3301 tatgagaaac agctttaggt agttgtgggt gacctggacc caaggcacga gttgctcctt
     3361 ttcgatgaac aaataagcgc tttttcttgc catctatctc atgaaattct tctttacaag
     3421 tattgtgaga aacatcatag attagatcaa tctgagtgtg gggtaaaata tcctgaaaca
     3481 cattgcgcat aaaatgagtg atgatttctc tgttggctaa agcacaatta attcctgctc
     3541 tcatagcgcc caggtaattt tcccctagct ttgaatgaat aggggcacaa gccaattccc
     3601 tatccgccag cctgatacca taatgttgtg attcaataac catggaacgg aggtaatctg
     3661 taccaatttg atgtcctaat cctctggaac cacaatggat actcactaca atatcccctt
     3721 catgcaaatt taaagctgtt gctgtatcgg aacagtagat ttgcctgact tcctgcactt
     3781 caagataatg attacctgaa cccaaggtac ccatttcatt tttctggcgc tttttagctt
     3841 gctcagagac aaaatcaggg atagctcctt ccacacaacc gcgatcctca atacgctcta
     3901 aatcagcagc ttcaccatag ccttgtttta ctgcccactg tgcgccccct cgcatcatat
     3961 catcgagctg tttgatagtt aaatgaatgc cgcttctact acctactcca gctggaatat
     4021 gtgtaaataa agcgtctgca aggctaactt tgacagactc caattcatct cgcttcaatc
     4081 cagtagataa gagcctcact ccgcatgaaa tgtcaaaacc cacaccacct gctgaaataa
     4141 ctcctccatc atccggatca aaagcagcga caccgccaat cggaaaacca tatccccaat
     4201 gcgcatcagg catggcataa gcagcagaaa caattcccgg caaagtagca acattagata
     4261 attgttcgta aactttcata tccatatcaa gaatcagctg ttctgaagcg aaaataactc
     4321 ctggcaccaa catctttcca tgttgaggta tttgccattc aaaatcattt atttttttca
     4381 agagacttaa atccatcact gtccaccaca ttaatacctg ttaaggagtt tatacatcaa
     4441 caacacattg agctatccaa atgtcattat ttttgtatac ttttaattca gtataggtag
     4501 cccctttaac ctcaacaact ggttgatgct ttttttgatc aaccttttca cccttaatga
     4561 cagcatccaa agcgaagcga ttgagtttta catcaaactc acgaaacagc atattatgaa
     4621 tttgcatatt ataaaccacg gcatttagcc aatcggtaaa taaaatttct tcattgggtg
     4681 cttcacaatg tatttttatt gataaagaag gcttgattag ggttgcatct gcaactacat
     4741 tcgttaatgc caaagccccc atttcaaagg catctgtgag tgtaggtccg atgccgcgaa
     4801 ctccaatatc tgcctcgtga gtgaaatgtt cccagtattt atttaatggt aacgggtttt
     4861 gtcttttgct aataatcgca actccttttt agcttccatt aaaatttctt tttgcccggc
     4921 ggctaatttg cttccagctc ggttaatgta aaaattcaac atagacatcg ctgattgata
     4981 aggtgtacct ttgcgcctat tgctgtttaa tgccgagtta agcaaagaaa cagcaatttt
     5041 ttggggatca ttccaggtaa ataccccttt ttcgagatca agcgcattgc ttgtattcgt
     5101 cactctttgt gaccaaaact ttcccattga ttcgaccaac tgaatcccat acactaatat
     5161 tatagcgatt taatataagc cttgttgttt tgagtcaggg caaatgccat ctaacttaaa
     5221 gtataagtgc ctagaagttc agtttcgccc atctcctcgc cgtttaagac aatgagtgat
     5281 actttatata gaccagaagg caaatccttt cgcgagatag ttccgttaaa ccctgacaaa
     5341 tcaagatgct taccatcctg tttgaaagcc agattaacac tttgtttttg cattttattc
     5401 atactgtata aataagtatg agctcccatc gacaacttta aaaacacttc cccatttcca
     5461 gcatctagac cgggaatata agcccagcca ttgactgaga ttgtatctgg gccaatcttg
     5521 aatttatcga gctctcccct cgcagctaaa gcctgtttag gaagctcaag gctcacttga
     5581 tttgctgtag tttgaaaatt aacgagagga tagagcaaat cacgctcgac tttttcatac
     5641 acaatcaaat caggatggta tttttgaatt aaatcagaca aattccagga ggtattcccg
     5701 gagtgaatac gtactgtatt ataaaaatca ttatgaaaat aaaaactgag agactccgaa
     5761 aagctgtctc ctattaataa acaggtttgt ttgttttgta cagctttatt aatattctcg
     5821 taaggcgcaa tgaggattac accatttgga ttgccctgga aatcatccaa tttaatctct
     5881 tttccatcaa tgtctctgcc aaacaaatcg atttttaaat ttgaaacatc gggaagagga
     5941 ttattagatt taatatgtgt caactgcaga aaattggtaa gatcagagta ggtggtttct
     6001 ctgggtatga aatgaaactg taattgtctg ctctgtaacc ctttttgctg catgtaatct
     6061 gatatcgcct tgtaagcaac ataagcacca acataattcc aatgactatc cccctttaaa
     6121 taaagatctc catactgttt accaagagta tttttggctt caatttcctt ttgtctcaaa
     6181 ttgataaaat caatgcctcg agcaagcatt ccctcttgta aaaggtcaag cctgttctta
     6241 ttggagctct tatgaacgtt agccggtaaa tattccggat aaatctcatg tttatctggg
     6301 gcaatggcca ccagaaaagg aatgttattt tgcttagcca gatgattcat ctgttttaag
     6361 acagataatt tcaagagaat ttcttcctcg gtaggcttat ttctccctgt gtactgatcg
     6421 atggatgccg cgtaatcatt gcccaaaaac agccaatcct ctttccctaa aatggcttgc
     6481 ccaggtttga tgctggctcc caactgatat acgacatgac tccagaattt actcaacgag
     6541 gtcatgaaca aagtatgatc aacaaaccaa ttttctatgc ccttgaaatc agtacgacct
     6601 atttttttta tattaatggg ggggagttga tgaggttctc tgtttaatag agatctcact
     6661 tcatccgctt gtttttcaaa aaacatacca atccctggta caaaaatgat taccagaaaa
     6721 gcaaagatga ccaacctgtc agaaaacctg gaaaaaatac gcatcatcaa aacctaaaat
     6781 agaggaaagg gttataagaa ttagtgctta ataataatat tgaaaccagt aaaagcagta
     6841 ttcccaaaac cttgctggat tgcttgtatg cgataatcca gtttttattt ctacttaaga
     6901 tgagactatc ccttattctg ctgttaaacc ctaacgctag tataatgccc acaataaaag
     6961 caaaaataag aataatatta atacatgctg ctatggaatc aagattgaac tcagcaggaa
     7021 taaacatcgt tttaaggaat gccagtgccg actgaatatt atcttctctg aaaaataccc
     7081 atccgactgt cacaacagaa agaacatagc catggcgaat aactttaggt aattttttca
     7141 taaaatagcc aaagtgggtt ctttccagaa taaggaaaaa cccatgaaat aacccccaaa
     7201 caataaaggt ataattagcg ccatgccata aaccacacaa accaaataca atgatcaagt
     7261 taatttgcgt tcgaaatctt gacttataac tccctccgag cggtatatat aaataatctc
     7321 gaaaccagct tgataatgag atatgccact tccgccagaa ctcctgtata gataaggcat
     7381 tatagggaaa attaaaattt tccttgtatt taaacccaaa catcaaacca aggcctattg
     7441 ccatatcaga atagccggaa aaatcaaagt agatttgcag agcataggct atagccccga
     7501 gccaggccac ccaaaaagtc aactcattgg caggcaaata aaatacataa tcagcaagct
     7561 ttgccacttc attggcgata aggacctttt ttgccaagcc gaaacaaaaa cgcagaattc
     7621 cattgcgcac tcccgcacga gttatatatc tccgatagaa tgaatgtctt atatctgcat
     7681 agcgaacgat tggtcctgcg accaattggg ggaaaaaaga cacataacaa acgaaagtaa
     7741 caaaattacg ttctgctggt acttttttcc aatacacatc aaccacatag cttaaagcat
     7801 gaaaagtata aaaacttatt ccaagaggca acacaatttt actgattccc atgacattta
     7861 agtcaaagac actgttcagt gagtcaagaa aaaaattaaa gtacttaaaa tacatgagca
     7921 tggccagtga tgcgctcacg gaggtgatca aatagatttt tcgttgtcct ggttttttgc
     7981 tgtcctcaat caataggcca catagataat ttatcaaaat gcacgccagc ataataaaga
     8041 tatgatcttt ctcaccccaa taataaaagg acaagctaaa gagcattaaa acaaaatttt
     8101 gccacttgat acttttaaaa cagactagat aagtcaggtt aaggcacgga agaaataaaa
     8161 aaagaaatat taaagatgaa aaaaccattc tatctccgtg aaggcatttg cggaattaat
     8221 taaacttgta ttaactgttc aagacgttag attcatcgcg tgaagcgcat tatagggaag
     8281 tgttatccgt tgtgcaaata gaatacgata aaaagaacca tctgcaaaat ctgaattgaa
     8341 gctgccatta taacattcat tagctatact taacctaagg cagcattcgt tgatgaatta
     8401 ataatgcaag attccttttt ctcattttta tcaagcccta tcaatttact ctttcttttt
     8461 gttgcatttt ttttgctttt tgtaacgcca tggctctgga gcatggtgaa ttcaactcac
     8521 aacattcaca agctgcttga ccttatcggg attaagaaaa aagaaaactc acgagtagtg
     8581 aatggactgt tacttcttcc actgttactg ataacgattt tcattgagtg gctgtggcct
     8641 ttggcaaata tcacgttgtt tccgatactg gcctcaccct atattaaatt taatattatt
     8701 gcagtcattt cagtaattat tcttttgatt ttaatccaat ttgataaaaa aaaatcaatg
     8761 gggctttggg tgcttgccta tgcagctgta ctttatttat tgtatttgat tatcatcatg
     8821 gcgacagtag tggtagggat gaatactttt cctgcaagcc attatatttt tgcccctgaa
     8881 agcagcatga caatcaatag gacaataatt tatggtttat taatagctca tttaattttt
     8941 atctggctaa tcatatcgct taaattcaca tggcgtttat tgttaatgat actctatact
     9001 ctggccctat catttgctta tttctctctt atagtctctt gaataagctg tatctttcat
     9061 tttattgcag tgttcaaacg tttctgtggt gctcatttga catagtaaac ttcactccct
     9121 aaacgtttgc accttaccct aaagcaaaat aatcaggtta ggcaagaagt ctaatataaa
     9181 gccattgcct tcatgataaa accaaggtat acctagttta ccagattacg gtcatcgaca
     9241 cagaacaata aaaaattttt acaggatgta gcattcatga accgcatggc acttttcatc
     9301 atttttatta ttcattgtta tttttctcaa tcatttgcgg aacaggaaaa accatacaac
     9361 gaactgtacg tcaagcaagc aaacctgaaa cagtatccca aagaaatcaa tagttatcca
     9421 cctggtgtag aaatcactat tggagatttg catggaaatg cattgaaatt gctctatttt
     9481 ttaattcgca atgatgtgat taaaatcgac aaagaggact ataagttatt tgtaactatc
     9541 tatcagaaaa atcctaatga actgacaaca aaagatttgt cttttttcca aattatagtc
     9601 aattccgctg aaatcaatac ccaacacaag atacgttttc tgggagatga tctatgtgat
     9661 cgtggtatga atgactatta cactttggtt atttataaaa aactcgacca ggccaatgtc
     9721 ccttttgagg tgattctatc aaatcatggc aacttttttt taacagccta tgaaagacct
     9781 gagcaatctt ttaactataa tccttacggt gaaggtgaaa acgaatcgac agtgcaatcc
     9841 atgctaaata tgggaagact gattgatcgg ggattgattg ataaacagga tattttagaa
     9901 atgattcaat atcattactt aaagcatata gtgcttcctg gttatacgca taataaagat
     9961 aaaagtgagc ttactattta tactcatgcg ccaatcgatt taggaataat ctcaacatta
    10021 gctaatgatc tgcaagttcc ttttaaagac tctaacttgt atgaattaac caaaagtctt
    10081 gacgcaatta actccaaaat aaaacaatgg atactctcta acacgttcac tagacattac
    10141 aaggagctta acgaagcaca caatcagacc aacacgccaa gcccaattaa gcaaatattg
    10201 tggaatcgtg attattctat cctcgatcgc cacgctaatc ccaataacaa accctatggc
    10261 attaattatg ttcatggcca cgactctatg cccaatgtgt ttgatctgga taacttattt
    10321 ggaaaaggtg aggattttta ccaagggcct tacgctgtgc atattacaca cagctagctg
    10381 tgaataaact caaccctgtc attaaacagg gtaacaaatt gaaattatag ccctagccac
    10441 cgaataaagc ggtttgttgt ttttaaattg agggctggtc ttgattcaaa atctattcta
    10501 tagcttttag caggttcctg tgtaatacga gacgtatcag aattgggttg aacttcctta
    10561 attttaccca tattgacgat ttgcatttga gtttcataat acccatgttc cataataatg
    10621 acaggtatta caaaatgaat ttctcccaaa ccctccctat tcccctgaag aaccagcttg
    10681 ttaaattctt ccttactcaa agcaatgtat tctgcattgt taatttggcg tcttaacatg
    10741 cgggtcaaag gaggttgaga atgaaaaata cgctcataag catccgctga catagtaatg
    10801 ataggttcat gctcttcacg gctgttattt ttattgaggt caacaaacaa aatatcttcc
    10861 cgtatttcat cgtcaacagg agcttgggtc tcagaaagcc tattaatagc gacttgatca
    10921 gatttctgtt tactgcaatt taaaaatttc gacttattaa accactcatc gctaggataa
    10981 taaacaacca gtaaagaatt atcttttata gtatcaataa tttgcttttt aattggcaaa
    11041 ggtaatgcag gacagcccca actacggcca ggccgaccat atcttttgat gaattgttca
    11101 tccatatacc atccgccatg cataacaata tagcgattaa aggcattatc attaaacttg
    11161 gtatccaaac ccactaatcg taaagacaac ccttctcgac cataatagac ttgctctgtt
    11221 ttataaacac caatactgct ggccttacta tcaaatttat ttgaaaattt atccgttaaa
    11281 agagcccctg attttatccc gtgagataca taagtatgaa ataataattc tttcttatcc
    11341 aaatcaaaca cccataaccg tttttgatta gaaggcatag aataatcaat aatagtcaat
    11401 atattatttc tatccacttg ataagcatta gcgcattgta tggtcgttat cactttatca
    11461 ataacggcag gttgaagaga attaccctct ttttgtaaca atgtttttat ttcattcaaa
    11521 ggcatcttga ttgacggtat ttcactgtat aaaagattcc cttgaggctc ggcttttgac
    11581 actggaatcg tagaagttga aaaattaaat gat
//
LOCUS       DASLOE010000091        11549 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.092, whole genome shotgun sequence.
ACCESSION   DASLOE010000091 DASLOE010000000
VERSION     DASLOE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11549
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.092"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..669)
                     /locus_tag="U6309_001889"
     CDS             complement(<1..669)
                     /locus_tag="U6309_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEN5505328.1"
                     /translation="MMTKTNEENLTWKGKQKQRARAWIKIFLEKWFRIEVKGQYQANS
                     HSVIIANRASIIDVLLLSVFLPERLTLALHPDMFKKIWVKTLLFFADVIVVDPGSAQA
                     TRILIRAIRAGKRCLLFPQGLLRQQEDSLKVFEGPGLILQRVGAEVIPVRIEGAEHSI
                     FSNSKNKHRIRIFPKITLHILPAQKLSQAENTGVDREGISLRLFQLISDLSFANSFKP
                     YVLFS"
     gene            824..1507
                     /locus_tag="U6309_001890"
     CDS             824..1507
                     /locus_tag="U6309_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN5505329.1"
                     /translation="MRVLNSCNKVRGIFTLTQKTIKTFCFILAILVLIIFAVVFHNHS
                     LEIIDYIDDLGWLAPVLFLITYCLATLLLLPTMVLTLAGGAVFGPVFGTLLNLLGATS
                     GAALAFLITRHLVYDWFSTKKGEKLNKLIAGVDEKGWVFVAFLRLFPIVPFNLVNYGL
                     GVTGISFRLYLLTTFIFLIPAEIIYTYFGYVGMDALTRPGHLYRNGGIFLTGLAIFFL
                     LVIRIMKKR"
     gene            complement(1534..2349)
                     /locus_tag="U6309_001891"
     CDS             complement(1534..2349)
                     /locus_tag="U6309_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEN5505330.1"
                     /translation="MPTRVIEDKGDMTPSFGIDDRIFLGEGLFETIRVNSSKPSFAYM
                     HWERLGNSARQLGIPFEISFDDWFEHLIQKIQKDNLYHGGIKAILSGGPASRGLAERG
                     QVSQLIFQTFNYSIQKHPVRLISINWLRDKANPLYQLKSVNYLEAIIAQRQAIAVGAD
                     DALFFNTENHVTETTCANLFLIENNILYTPRVEDGILPGITRARLISHCQQHKMSVQE
                     ISLTKKRIEDADAVFLTNSLQGIRRVLSLDNIIFEVNHPIIDKLIFLLNQDES"
     gene            complement(2363..2548)
                     /locus_tag="U6309_001892"
     CDS             complement(2363..2548)
                     /locus_tag="U6309_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505331.1"
                     /translation="MSDLFEEDEEEVIEVVDDFDDVEVDTEIIEAPGVSLDARRRLEN
                     MLEEKRLRDELDDFVDY"
     gene            2795..3376
                     /locus_tag="U6309_001893"
     CDS             2795..3376
                     /locus_tag="U6309_001893"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEN5505332.1"
                     /translation="MSDQYKVALRNTIKQVRSKLSVPYRTASSNQICTRIRSLEQYRQ
                     AKRIALYFAVNGEVDLTFLWKSAPLQGKYCYFPVLNNDSTLSFLPATPSTPFKTNKYD
                     IPEPDVDIELALPIDELDLIIMPLVAFDAHCTRLGMGSGYYDRTLANKSKAHLLGVAY
                     QFQRVDYIEPRPWDVPLDAVITQKAIYWRNPLA"
     gene            complement(3386..3724)
                     /locus_tag="U6309_001894"
     CDS             complement(3386..3724)
                     /locus_tag="U6309_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEN5505333.1"
                     /translation="MKMITAIIKPFKLDDVHEALMEIGVPGITISETRGFGRQKGHTE
                     LYRGAEYVVDFLPKIKIELALPDNMVEAAIEAICKSAYTGKIGDGKIFVYALEQVVRV
                     RTGEVGTDAL"
     gene            3869..4129
                     /locus_tag="U6309_001895"
     CDS             3869..4129
                     /locus_tag="U6309_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEN5505334.1"
                     /translation="MFDPKQFDELANKLFATLPTSLQNFEKDIQQKFKEVLQSTFSRM
                     DLVTREEFDVQCKVLARTREKLEQLQHQLEDFIKRQEVKNNK"
     gene            4194..5705
                     /locus_tag="U6309_001896"
     CDS             4194..5705
                     /locus_tag="U6309_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEN5505335.1"
                     /translation="MNLAFTKTRSTIGIVAQPVSVEVHLSNGLPGFTMVGLAETAVKE
                     SKDRVRSAIINSQFEFPCRKITVNLGPANLPKTGSGFDLPIALGILAASEQIPLTNLA
                     NHEFIGELALSGELRGVSAIIPAVLAAHKDNQHLIIANANAAEASLTGHPKVFTANNL
                     REVCDYLCQGTSLQNLPPKPDLLLNNHELDWSDIKGQHHAKNAMVIAACGGHSILLSG
                     APGSGKTMMAKRFSTLLPELSETQALECAAINSIRGKLPDFREWRLPPFRAPHHTASP
                     VALVGGGNPPKPGEISLAHHGVLFLDELPEFNRQVLETLREPLESGHICISRAAAQIE
                     FPAKFQLIAAMNPCPCGQWGNSQANCLCTPDRISRYLAKLSAPLLDRIDMQVTIHALS
                     QEELIKPNTHLEKQSPAIREKVTKMHEIQMARQDSLNANLNSKTCEMVCELGSEEQLF
                     LREVMSKLKLSARGYHRLLKVSRTIADMSECNKVMLNHLQQALSYKQILHLPK"
     gene            5810..6196
                     /locus_tag="U6309_001897"
     CDS             5810..6196
                     /locus_tag="U6309_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505336.1"
                     /translation="MRSIRLLFPCILLMIPWQFATANRPTDNALAIIKSCYDESVNLS
                     NEFTPCVTKKLKTMPNAENFQLIMNFKTDEKGTNERIPVFMVDKTGYMYYCIVTTGKT
                     LIINACAGEQGKPLTQGQMLSIDLPE"
     gene            complement(6310..7203)
                     /locus_tag="U6309_001898"
     CDS             complement(6310..7203)
                     /locus_tag="U6309_001898"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEN5505337.1"
                     /translation="MKHFLEISQLSSEQIESLLQRALYFKHTKQYPSYSQSIIANLFY
                     ENSTRTRISFELAERHLAMSVVNLDLQTSSETKGEAIEDTIRTLAAMGIQYFVIRHKQ
                     DGLQQNLANKLGDTVNIINAGDGTHAHPSQAILDMVTIIEQKKRLDKLKIAILGNIKH
                     SRVANSFQCICSKLGVGELVLISPEIWQPSQVHFGRVTDNLNEGLEGADVVICLRVQK
                     ERLLQDDHLDLDFYRNNFALTQKSLSYAKPDAMVMHPGPMNRGVEIDSEVADGNQSCI
                     LQQVTNGVYARMAILESLIAS"
     gene            complement(7253..7672)
                     /gene="ruvX"
                     /locus_tag="U6309_001899"
     CDS             complement(7253..7672)
                     /gene="ruvX"
                     /locus_tag="U6309_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEN5505338.1"
                     /translation="MPRGVYLGFDFGYKRIGVAVGQRLTCSASPLSTIEAKAGIPDWN
                     TIQKVITQWNPQALIVGLPTCIDDRELYTTSAARRFAKQLHKRFSLPVHLVDERLSTV
                     EARGYLFEQGGYRQIKKAEVDSIAACVILEQWLQQSE"
     gene            complement(7665..8228)
                     /locus_tag="U6309_001900"
     CDS             complement(7665..8228)
                     /locus_tag="U6309_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEN5505339.1"
                     /translation="MAIISSLANHLLIAMPSLKDPNFERSVVYLCEHNEQGSVGLIIN
                     RPLQFPLSIVFEQLQIEPIRVEKNGLPLLFGGPVQPERGFVIHKQMGGWRSSLFLQDE
                     VTVTTSNDIIRAIAYDEGPKDVLITLGYAAWTEQQLEREIMSNTWLVCPYKSEILYEV
                     PFEERWEYAGLTLGIKMNQLSSDAGHA"
     gene            complement(8531..9295)
                     /locus_tag="U6309_001901"
     CDS             complement(8531..9295)
                     /locus_tag="U6309_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/III secretion system protein"
                     /protein_id="HEN5505340.1"
                     /translation="MIKRWLLVILLISGNAFSQQMITKVIELNYLQADKVIQLIQPLL
                     QTDEKVSGSGQTLIVNVSPQTLTQIRNVLHKIDVPPVVFNITVYQGDPEWLSSQDGND
                     VVYSTQPQYEVQRSQSVKVMSGESAFVATGEQVPVVTSVGVGLLATGVSYEQHNIQNG
                     LLVHPVLQGSQVKLTVRRVREQENVESDQQFDNQQIDTTIMAPLNKWVPLGTAEGVQN
                     TDSSSTSYTAGRPFSQNSTLYIKVSVVKSTPSGTTK"
     gene            9501..10154
                     /locus_tag="U6309_001902"
     CDS             9501..10154
                     /locus_tag="U6309_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HEN5505341.1"
                     /translation="MFGPSPIKPIEQHIRKAHQCAKQLYPFFEAVLKNDWDTANKIKD
                     KIISIEKEADLIKRDLRLHLPTGLFLPVARTDILELLSAQDRIANKAEDIAALIISRQ
                     MSIPKKLIPSFMPFLSRCLDASKQACTAINELDELLETGFRGSEVKIVEEMIVKLDEI
                     EHDCDERLADIRHKIFELEKELPAIDVIFLYKLVQWIGELADHAQTVGGRLQILIAR"
     gene            10164..11417
                     /locus_tag="U6309_001903"
     CDS             10164..11417
                     /locus_tag="U6309_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEN5505342.1"
                     /translation="MDYSIYLLFAALILCFLMTWGVGANDLANVMSTTMGSKAVTVRQ
                     AMLIAIIFEFAGAFLGGEGVTETMRDGIINTSQLSNEPLILIEGMLCVLFACTIWMNL
                     ASYLGVPVSITNALVGSMVGFGTIVLGPDAIHWNQVARIAIGWISSPLISGITAYALF
                     ISIQQTIFVKSNPLTKAKLYIPIYLFLIGFILSFITVFKGLNHFDIHLNLKQDLAVTL
                     ATSIIITILGMIFIKRIPEYHKIRRRERFIQVEKYFAVLMAMTACAMAFAHGSNDVAL
                     AVGPLSIVHSLVMHSNQIFDADNYPSWIILLGCVGVVTGFLMYGRKVIETVGSSITAL
                     TPSRAFAATLSAATTVVVATSTGIPVSATQTLVGAVLGVGLARGIGALNLIVIRNIFM
                     SWVLTLPAASMLTILSYKLLHALLG"
BASE COUNT         3525 a         2380 c         2067 g         3577 t
ORIGIN      
        1 agaaaataaa acatatggct taaaactatt ggcgaatgat aaatcgctta ttagttgaaa
       61 taaacgtaaa ctgatacctt ctctgtcgac acctgtattt tcagcttgac tcaatttttg
      121 agcaggtaat atatgaagag taattttcgg aaaaatccgt attcgatgtt tatttttgct
      181 attagaaaaa atactgtgct ccgccccttc aatacgaaca gggattacct cggcgccaac
      241 cctttggagt atcaatccag gtccttcaaa gacctttaag ctgtcttcct gctgtcttaa
      301 taacccttgt ggaaatagaa gacaacgctt gccagcccgt attgctctta ttaaaattct
      361 tgttgcctga gcgctgcctg gatcaactac tatgacatca gcgaaaaata ataacgtttt
      421 tacccatatt tttttaaaca tatcaggatg taaagctaaa gttaatcgtt cgggtaaaaa
      481 tacagaaagg agtaacacat caattataga agccctgttg gcaataatga cagaatgact
      541 gttcgcctga tattgtcctt taacctctat gcgaaaccat ttctccagaa aaatttttat
      601 ccatgcccta gcccgctgtt tttgttttcc cttccaggtc aaattttcct catttgtttt
      661 tgtcatcatg tcgcatcagt tatctctata ttgactttta ttctgcaaga ttcaaaacga
      721 tttgaataga taggtttatg aatcatgttg atttcattta aaaaatttaa gcgaaagcaa
      781 catctgtttt tatgataatt tacccggaat attgagagaa ggcttgcgag tccttaattc
      841 ctgtaataaa gtgaggggca tctttacttt aacacaaaaa accattaaga cattctgttt
      901 cattttagcc atattagttt taattatttt cgcagtggtc tttcataatc attcgttaga
      961 gatcattgat tatatagacg atctgggttg gcttgcccct gttcttttct tgatcaccta
     1021 ctgcctcgcg actttattgc ttttacccac aatggtatta actctggctg gaggagctgt
     1081 gtttgggcct gtatttggta ccttgcttaa tttattgggc gccactagcg gcgctgcgtt
     1141 ggcatttctg ataacaagac acctggttta cgattggttc tcaaccaaaa agggtgaaaa
     1201 actaaacaaa ttgattgccg gggtagatga gaaaggttgg gtttttgtgg ctttcttgcg
     1261 attgtttcca atagtccctt ttaacctggt caactatggc ttaggagtaa ctggcatatc
     1321 atttcgtctt tatttgctaa ctacttttat ttttctaatt cctgcagaaa ttatttatac
     1381 ctattttgga tacgtcggca tggatgctct gacaaggcca ggccatttat ataggaatgg
     1441 tggaattttt ctaactggat tagcgatttt ctttttactt gttatcagaa taatgaaaaa
     1501 aagatagttg ttttttcaat taataaaaat ttatcaagac tcatcctgat ttaataaaaa
     1561 gatcaactta tcgataattg gatgattgac ttcaaaaata atgttatcca gggagagcac
     1621 ccttctgata ccctgcagac tattagttaa aaaaacagca tcggcgtcct caatcctctt
     1681 tttcgttaat gatatttcct gcactgacat tttgtgttgc tgacaatgtg aaatgaggcg
     1741 tgctctggta atgccgggta agatcccatc ctctactcgc ggggtataca aaatgttatt
     1801 ttcaatcaag aataaattgg cacaagttgt ttcagtaaca tgattctctg tgttgaaaaa
     1861 taaggcatca tctgccccaa ccgcaatcgc ttgacgttga gcaataatcg cctctaaata
     1921 attcactgat tttaattgat aaagaggatt ggccttatcc cttagccaat taatactgat
     1981 taaccgaaca ggatgctttt gtatagaata attaaaagtc tgaaatatta attggctgac
     2041 ttgtcctcgt tctgccaagc ctctcgaagc aggaccacca ctcaaaatgg ctttaatgcc
     2101 accatggtac aggttatctt tttggatttt ttgaataaga tgctcaaacc aatcatcaaa
     2161 tgaaatttca aaaggaatac ccaactgacg agctgaattg cctagtctct cccaatgcat
     2221 ataagcaaag gaaggcttgg aactattgac tctaattgtt tcaaataatc cctcacctaa
     2281 aaaaattcga tcatcaattc caaatgatgg cgtcatatct cctttgtctt caataacacg
     2341 agtaggcatt atgatctcat aattaataat ctacaaaatc atctaactca tctcgcaatc
     2401 gcttttcctc caacatattt tccagtcgtc tgcgcgcatc caaactgaca ccgggagctt
     2461 ctataatttc agtatccact tcaacatcat caaaatcgtc gacaacttca attacttctt
     2521 cttcatcttc ttcaaacaag tcactcataa cattccctca aagaattaaa agtaacatta
     2581 aataattgct taaatggaac aagattccag gacggctata taccttattt ttaatttttt
     2641 gcatagtatt ttttaattat tttatgttat aaacttatcg catcgcgcat ttataatcac
     2701 ccaatgcata aaacaacata ttgatacctg gaaacacttg aactttaagc ctaatccatc
     2761 taataataaa aaagaatatt agtgatacca aatcatgtca gaccaatata aagttgctct
     2821 aagaaatacc ataaaacagg taagatccaa gctttctgta ccctacagaa cagcatcttc
     2881 caatcaaatt tgtactcgta tccgctcgct ggaacaatac aggcaagcaa aacgaatagc
     2941 cttgtacttt gccgttaatg gagaagttga tttaacgttt ctctggaaaa gcgcgcccct
     3001 ccaggggaag tattgttatt ttccggtatt aaataatgat tccactcttt cttttttacc
     3061 agccaccccc agcacccctt ttaaaacgaa taaatacgat atcccggaac cggacgttga
     3121 tattgagttg gccttgccta ttgatgaatt agacttgatt atcatgcctc tggttgcttt
     3181 tgatgcacac tgtactcgtt tgggtatggg ctcaggctac tatgatcgca ccttggctaa
     3241 taaatcaaaa gcgcatctct taggagtcgc ctaccaattt caaagggttg attacataga
     3301 accacgccct tgggatgttc ccctggatgc cgtaataact caaaaagcta tttattggcg
     3361 aaatcccttg gcctaaaatg acattctata gagcgtctgt accgacttcc ccggttctaa
     3421 ccctcactac ctgttccaaa gcataaacaa aaatcttgcc atcaccaatt ttaccagtgt
     3481 aggcggactt gcaaatagct tctatagctg cttcaaccat attatctggc aatgccagtt
     3541 caattttaat tttagggagg aagtctacca cgtactccgc ccctcgatat aattcggtat
     3601 gacctttttg acgcccaaat cctcgagttt cagatatagt aattcctggc actcctattt
     3661 ccattaaagc ttcatgtaca tcatccagtt taaatggttt gataattgcg gtgatcattt
     3721 tcatattttt ttccatcacg ttaaatgcag agccacccat gatatttcta acacacaatt
     3781 tttatgttat tgcttttcaa gacatcttgg gtatagttgt aatccaacag ttttgctaga
     3841 ctgtattcaa aaataatccg gagccaccat gtttgacccc aagcaatttg atgaattagc
     3901 taataagctt ttcgctactt tacctacgag tctacaaaat tttgaaaaag acattcaaca
     3961 aaaatttaaa gaagtactgc aatcaacatt cagccgaatg gaccttgtta cccgtgaaga
     4021 atttgatgtg caatgtaagg tacttgccag aactcgagaa aaactggagc aattgcagca
     4081 ccagcttgaa gacttcatta aacggcaaga agttaaaaat aataaataat aactattagt
     4141 cgtccatagg aaatgggatc gacttaagtc caacataata aggaaaaaat cttatgaatc
     4201 tcgcatttac caaaacgcga agcactattg gtattgtcgc acaaccagta tctgtggaag
     4261 tccatttatc aaatggatta ccaggtttta ctatggtagg acttgctgaa acagcagtga
     4321 aagaaagtaa agaccgagtg cgcagcgcta ttataaatag ccaattcgaa tttccatgcc
     4381 gtaaaattac cgttaatctt ggccctgcca atttacctaa aacgggaagt ggctttgact
     4441 tgcctattgc actagggata ctcgcagcat cggaacaaat tcctctcacc aaccttgcaa
     4501 accacgaatt tattggagaa ttggctttaa gcggcgagct tagaggggtt tctgccatta
     4561 tacctgctgt tcttgccgct cataaagaca atcaacatct gattatagcg aatgcaaatg
     4621 ctgccgaagc ttctttaacc ggccatccaa aagtctttac tgccaataac ttacgcgaag
     4681 tttgtgatta tctatgccag ggtacttctt tacagaactt acctcccaaa ccagatcttt
     4741 tattaaataa ccatgaattg gattggtctg atattaaggg gcaacatcat gccaaaaatg
     4801 ctatggtcat tgctgcctgt gggggacaca gtattctcct aagtggcgca ccaggcagtg
     4861 gaaaaacgat gatggcaaaa agatttagta ccttgctacc tgaactatcc gaaacacaag
     4921 ctctcgaatg cgcagccatt aattcaattc gaggcaaatt acctgatttc agggaatggc
     4981 gtttgcctcc ttttcgtgca ccacatcata ctgcttcacc tgtcgcttta gtcggaggag
     5041 gcaacccgcc gaaacccggt gaaatttcat tagcccatca cggcgtatta tttttagatg
     5101 aattacctga atttaatcgg caagtattag agacacttag agaacctttg gagtcagggc
     5161 atatttgcat ttccagggca gctgcacaaa tagagtttcc tgccaaattt caattaattg
     5221 cagcaatgaa tccttgccct tgtggccaat ggggtaattc acaagccaat tgcttatgta
     5281 ctcctgatag aatcagccgc tatttggcaa aactttctgc tccattatta gatagaatcg
     5341 atatgcaggt tacaattcac gcattatctc aagaggagtt aattaaaccc aatacccatt
     5401 tagaaaaaca aagtcctgct attagagaaa aggtaacaaa gatgcatgaa atacaaatgg
     5461 cacgacaaga tagtctcaat gccaatttaa attccaaaac atgcgaaatg gtttgcgaat
     5521 tgggatcaga agaacaattg tttctccgag aggtgatgag taaactgaaa ctgtctgctc
     5581 gtggttacca tcgcttacta aaagtaagca ggacaattgc tgatatgagt gaatgtaata
     5641 aggtcatgct aaaccatctg caacaggcgt tatcctataa acaaatcttg catttgccta
     5701 aataaaattt cttctcaagt attggggtgt ataaaaaatc aattccgtat taatcatata
     5761 tacctaagtt gtcctataat tatacataaa tataaattac ggggccatca tgagaagcat
     5821 acgactctta tttccttgca ttttattaat gataccatgg caatttgcaa cagctaatag
     5881 acctactgat aatgcgctag cgattataaa atcatgctat gatgaatcag taaacctcag
     5941 caatgagttt actccctgcg ttaccaaaaa actcaaaact atgcctaacg cagagaattt
     6001 tcaattgatt atgaatttta aaactgacga aaaaggcacg aatgaacgaa taccagtctt
     6061 tatggtcgat aaaacagggt atatgtatta ctgtatagta accacaggaa aaactttaat
     6121 cataaatgct tgtgccggag aacagggaaa accgctgacg caaggtcaaa tgttatccat
     6181 cgatctacct gaataatttt gcggtttcct atctattgct caacgtggtt aatcatgcgt
     6241 cttgcagtat gagtagaata tattacccaa gagaataaaa gcataatgca tgacagaata
     6301 tagagaaacc taactcgcta tcagcgattc aaggatagcc attcttgcat aaacaccgtt
     6361 agtaacctgt tgtaaaatac aagattggtt gccatcagcc acttcactat caatttcaac
     6421 cccgcgattc ataggcccag ggtgcataac catagcgtca ggttttgcat aagacagact
     6481 tttttgagta agtgcaaaat tgtttctata aaaatccaaa tccaaatgat catcttgcag
     6541 caaacgttcc ttttgaactc gcaggcaaat taccacatcc gcaccttcca gaccttcatt
     6601 gagattatca gtaacccgtc caaagtgcac ttgtgatggt tgccaaatct ctggagaaat
     6661 caaaaccaac tcaccaaccc ctaatttact gcaaatgcat tggaaagaat tagcaacacg
     6721 tgaatgttta atatttccca aaatagcaat ttttaattta tctaatcgct ttttttgttc
     6781 aataattgtc accatatcca gtatcgcttg gcttggatga gcatgagttc catcacctgc
     6841 atttattata ttaactgtat cacctaactt attagctaaa ttttgctgca gcccatcttg
     6901 cttatggcga attacaaaat actggatacc catagcggct agcgttctga tggtatcttc
     6961 aatggcttcg cctttagttt ctgatgaggt ctgaagatcc agattcacta cagacattgc
     7021 cagatggcgc tctgctaact caaagctgat gcgggttcgg gtactgtttt cataaaataa
     7081 attagcaata atactttggg aataagaagg gtattgcttt gtatgtttaa aatataaagc
     7141 tcgttgcaat aaggattcaa tttgttcaga tgacaattga ctaatttcaa gaaaatgctt
     7201 catgttcccc cgccaaattt cattttacgc caaaagaaaa atactctttg agttattccg
     7261 attgttgcaa ccactgctcc agtataacac aagctgctat actatctacc tcggcttttt
     7321 taatctggcg atatcccccc tgctcaaaca aataccctct tgcctctact gttgaaagtc
     7381 gttcatcgac taaatgcaca ggtaaagaaa accgcttgtg caattgcttt gcaaaacgac
     7441 gagcagctga tgttgtatac aattcacggt catcaataca ggtaggaaga cctacaatta
     7501 aagcttgtgg attccattgg gttatcactt tttgaatggt attccaatca gggattccgg
     7561 ctttagcttc tatagtagat aagggagagg cactacaagt tagccgctgt cctacagcga
     7621 ctcctatgcg tttatacccg aaatcaaaac ctaaatacac acctctaggc atggccagca
     7681 tcagaagaga gttgattcat tttgatgccc agcgttaatc ccgcgtattc ccaacgctct
     7741 tcaaagggca cttcataaag aatttcagat ttatagggac aaactagcca cgtgttactc
     7801 ataatttctc tttccaattg ctgctctgtc caggcagcat aacctaaagt aattaataca
     7861 tctttaggtc cttcgtcata agctatggca cgaatgatat catttgaggt agtaaccgtc
     7921 acttcatctt gcaaaaacaa actggaacgc cacccgccca tttgtttatg aataacaaat
     7981 cctctttctg gttgtactgg tccgccaaac aataaaggca acccattttt ttctactcga
     8041 ataggttcaa tttgcaattg ctcaaaaaca atagatagtg gaaactgtaa aggtctgtta
     8101 ataattaaac caactgaccc ttgctcatta tgctcgcaca aatagaccac tgacctttca
     8161 aagtttggat cttttaagga aggcatggca atcaataggt gattggccag tgaactgata
     8221 attgccataa gaagtccccc ccaaaattat ctccgctcac tttaattata cactattaat
     8281 gattttaatt ttatcatttt gtttttaatt taatctaccc atgatggcat gagctcacaa
     8341 taccgaatta tgatgatttt catggcttaa agatgcttac gagaagctcc aggatcaagg
     8401 caaaaatatt tttaatgaag ggattaagag ttagttaatc agtaaatcct ggcttattct
     8461 ccagttcagg gaagaaaagc tcattaagag aagtccaagt aaaatgatta tgaaacgcat
     8521 ttccatcatt ttatttggtg gtcccagaag gggtggattt tacaactgat accttaatgt
     8581 acaaagttga attttgagaa aaaggccttc cagctgtata agaagttgaa gaagaatcgg
     8641 tattttgaac cccctctgct gtacctaatg gaacccattt attcaatggc gccatgatcg
     8701 tagtatctat ttgttgatta tcaaattgtt gatcactctc tacattttcc tgctctcgca
     8761 ctcttctcac cgtcaatttg acctgtgatc cttgcaaaac agggtgtact agcaaaccgt
     8821 tttggatatt atgctgctca taagatactc ctgtggctaa aagaccaaca ccaactgaag
     8881 taactactgg tacttgttca ccggtcgcaa caaaagcgga ttccccactc ataactttaa
     8941 cggattgact gcgctggact tcgtattgtg gttgtgtgct gtaaacgaca tcattcccat
     9001 cctgtgagct taaccattca ggatcccctt gataaacagt aatattaaaa acaacaggag
     9061 gaacatctat tttatgtaat acgtttctga tttgcgttaa agtttgggga ctcacattaa
     9121 caattagtgt ttgccctgaa ccacttactt tctcatcagt ctgcaataat ggctggatta
     9181 actgaataac cttatctgct tgcaaataat tcaattcgat aaccttggtt atcatctgct
     9241 gggaaaaagc gttgccagaa attaataata tcactaatag ccatcgctta atcatagaaa
     9301 cactcctgtt gtttcaattt aattataaaa tcctttattg aattgtagca ctaataggac
     9361 tcaaaaggtt acaaatagcc ccctttaatc cctaatctgt attcactcta gataagtagt
     9421 agttttgttc ctcaaaaagt ttaggtataa tccttaaaat ctgttatccg agatcaaaaa
     9481 ttatgggtag tatattcaat atgttcgggc catcaccaat aaagcccatt gaacaacaca
     9541 tacgtaaagc acatcagtgt gccaagcaat tgtacccttt ttttgaagcg gtgctcaaaa
     9601 acgattggga tactgcaaac aagatcaagg ataaaatcat ctctattgaa aaagaagcgg
     9661 atctaattaa acgtgatttg cgtcttcatc ttcctactgg cttattttta ccagtcgccc
     9721 gcacagatat attagaactg ctcagtgctc aagatagaat tgccaataaa gcagaagata
     9781 tagctgcttt aattataagc agacaaatga gcatacctaa aaaattaatt ccgtctttta
     9841 tgcctttttt aagccgatgt cttgatgcct ctaaacaagc ctgtacagcg attaatgaat
     9901 tagatgaatt gcttgaaacc ggattcaggg gcagcgaagt caaaatcgtt gaagaaatga
     9961 tagtaaaatt agacgaaatt gaacatgact gtgatgaaag actggcggac attaggcaca
    10021 aaatcttcga attagaaaag gaactcccag ctatagatgt aatttttcta tacaaactgg
    10081 tccaatggat tggcgaattg gctgaccatg cccagacagt tggtggtcga cttcaaattc
    10141 taattgcccg gtagttatca ctcatggatt attcaattta cctacttttt gctgctctta
    10201 ttctttgttt tcttatgacc tggggggtcg gtgccaatga tttggctaat gtcatgagta
    10261 ccactatggg ctcaaaggca gttaccgtcc gtcaagccat gcttatcgcc atcattttcg
    10321 aatttgcagg cgctttttta gggggtgaag gtgtcactga aaccatgcgt gatgggatta
    10381 tcaataccag tcaactctct aatgaaccat taatcctcat cgaaggcatg ctgtgcgttt
    10441 tgtttgcctg cacaatatgg atgaatcttg cgagttatct gggggtccct gtttcgataa
    10501 ccaacgcatt agtaggctcg atggttggtt ttggcactat agtcttgggt cctgacgcaa
    10561 tacattggaa ccaggtagcg cgaatcgcta ttggatggat ttcatctccg ttgatttcag
    10621 ggattacggc ctacgctttg ttcattagta tacagcaaac catatttgta aaaagtaacc
    10681 cgttgactaa agctaagcta tatattccta tttatctttt tttgattggt tttatattat
    10741 cttttatcac tgtatttaaa ggacttaacc attttgatat tcacttgaat ttaaagcaag
    10801 atttggcggt gactctggcc accagtataa taattacaat tctgggtatg atctttatta
    10861 agcgtattcc tgaataccat aaaataagac gcagggaacg atttattcag gtagagaagt
    10921 attttgctgt gctcatggca atgacggcat gcgctatggc tttcgctcat ggctccaacg
    10981 atgttgccct ggctgttgga ccattaagta ttgtgcacag cctggtcatg cattccaatc
    11041 aaatatttga cgcagataac tacccttcct ggattatttt attaggttgc gtcggagtag
    11101 taactggttt ccttatgtat ggaagaaaag tcattgaaac cgtaggaagt tctatcactg
    11161 ctttaacgcc cagccgtgca tttgctgcaa cactttctgc agcaactacc gtagttgttg
    11221 caaccagcac cggaatccct gtttcggcga ctcaaactct ggttggtgcc gttttaggtg
    11281 taggtttggc aagaggtatc ggcgctttga atctcattgt aatccgtaat atctttatgt
    11341 catgggtact gacattaccc gcagcctcca tgctaacgat cttatcctat aaacttttac
    11401 atgctttgtt gggataatcc tcattgccta tcagctcatc ataggcaatg gattgcctaa
    11461 atttcaacgc ctactgtaat atgaaaatga attgaatatc ctttaaaata taaattctca
    11521 ggcaaaccat atgcttttct caatcttgc
//
LOCUS       DASLOE010000092        11547 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.093, whole genome shotgun sequence.
ACCESSION   DASLOE010000092 DASLOE010000000
VERSION     DASLOE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11547
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.093"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            64..1035
                     /locus_tag="U6309_001904"
     CDS             64..1035
                     /locus_tag="U6309_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505343.1"
                     /translation="MLKKTSLALLGLAASGYALAGTMGPVCTPGNVTVPCEAKRWDLG
                     VQALYLKPVYDADMAYRQVHVAPTRYDDDQGWDWGYRLEGSYHFNTGNDATLTWIHYS
                     DFANPSGLVGRIDIPPLPPALAFPRLFSAIYKNRFEQVNLVMGQHADFGLVKDMRFYG
                     GLQYADIEIDNTNYYFGAFPQLGGVNLFDNTDYKGVGPVVGIDYAYALTNALSVTANG
                     AASVLYGKGKYNIGYVALPTGSSGAVVRSIYANKRTIVPSLEAKLGLNYAYNMTQGVL
                     NLEGGYQVMNYFNALHTQFLQNMANPVTNSDYGLYGPYIGAKYIGNA"
     gene            1331..2923
                     /locus_tag="U6309_001905"
     CDS             1331..2923
                     /locus_tag="U6309_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HEN5505344.1"
                     /translation="MKFKKIILALACLSSPLYADQDQQLKSEIQRLQHQAEDLQAQLN
                     RLEKQLANHKSSQQKHEQRAATKPAEPQSKPTVKSGAAIEEKYHSSKVEVHAPDAHPE
                     SISFYPTALIADNRVVTYIAGTPVVSSPFLGDRPAFDGSDYIVNISSINRDVRLLQQR
                     RRLYRAYQKIGYPIPNMPIISLSGKTEPAATFNNPFRSTNTDGDITLGSSELDVAAAL
                     NENVEAYIAIAYDESPPAIGPRVNNSAFNLNMGFVNIGNLDKSPLYFTAGQVYVPFGR
                     YSSAMVSSPVTMNLARTKTRPVIFGYKSQGDTGPFGAVYGYRSDTTLGRSGVGGVNLG
                     YIFGFDNDINGEIGGGFISSVADAGGMQSTGANVGTTFGGFGSITNGNENVRKTKAAD
                     VHGHVGYDRYNLTLEWVGAVQSFRPQDLSFNGQGARPQAAQAELGMTFMAFSRPASIG
                     VGYQWTKEALALNLPKQRYIGVFNISIWKDTVESIEYRHDIDYGLTQFANGAAPPGLV
                     NLPTLGTGKSADTVSAQIGVYF"
     gene            3138..4643
                     /locus_tag="U6309_001906"
     CDS             3138..4643
                     /locus_tag="U6309_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEN5505345.1"
                     /translation="MHRDCVRQEGLHKLNNLFCKWAAVTRTLQAIIFRSRLSVSVLED
                     SDSVAVKQSPPSVTCKIKLSKFMQTSYIFIFLTLFLLVGLQTSFAAKIVELNIKGPIG
                     PATADYLERGIKSAQDADLIVILIDTPGGLYDSTRNIIQLFLLSDVPIVTYVSPTGAR
                     AASAGTYLMYASTLAAMAPGTQMGAASPVSLGTGFSEGEKDEKKKSTMENKVTHDAVA
                     TIRSLAQLRGRDLDFAEKAVTEGKSITANEALSKGVVNYIAKNRDDLLFQINGIKVSQ
                     NNKTITINTENPDIQVINPDWRTRFLSVITNPTVAYLLLLLGIYGIFFELVNPGYVLP
                     GVVGAVSMLFALYALQLLPINYAGLGLIILGILFVIAEAFTPSFGALGVGGTVSFILG
                     SIMLMNTEHLAFQIAWSAIWAMAVLNILIFVLVLGMLIKSRNQKIRHGLETLVGAKGR
                     ALGDIHLEGQAVIKGEIWNVHSSSPIAANKSVKVTRASGLLLEVEEDQSVY"
     gene            4686..5441
                     /locus_tag="U6309_001907"
     CDS             4686..5441
                     /locus_tag="U6309_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEN5505346.1"
                     /translation="MGPFLVILLVAIGLLLASMFKVFREYERGVVFMLGRFWRVKGPG
                     LIIIIPIIQQVVRVDLRTIVMDVPSQDVISRDNVSVRVNAVVYFRVVVPENAIIQVEN
                     YFEATSQLAQTTLRSVLGQHDLDDMLAEREQLNSDVQKILDAQTESWGIKVSNVEIKK
                     VDLDESMIRAIAKQAEAERDRRAKVIHAEGELQASEKLLQASQVLAQQPQAMQLRYLQ
                     TLATIAVNNNSTIIFPMPMEIGDILTSMAGKKK"
     gene            5680..6246
                     /locus_tag="U6309_001908"
     CDS             5680..6246
                     /locus_tag="U6309_001908"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005889365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEN5505347.1"
                     /translation="MSIKSDRWIEKMALEHGMISPFQAGQVRENQNGRIISYGVSSYG
                     YDVRCSNEFKIFTNINSAIVDPKAFDENSFVDVQSDVCIIPPNSFALARTVEYFRIPR
                     NILTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTTLPAKIYAYEGVAQMLFL
                     EANEVCAVSYRDRNGKYQGQTGVTLPRT"
     gene            complement(6262..6573)
                     /locus_tag="U6309_001909"
     CDS             complement(6262..6573)
                     /locus_tag="U6309_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEN5505348.1"
                     /translation="MIKSELIEHIAARMTHLTEKQVADGINRILELMSEALIHGQRIE
                     IRGFGSFSLHYRPPRNAHNPKTGEKVVTEAKYSPHFKPGKELRERVNSSRAKFPRLDK
                     D"
     gene            complement(6826..7542)
                     /locus_tag="U6309_001910"
     CDS             complement(6826..7542)
                     /locus_tag="U6309_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEN5505349.1"
                     /translation="MLDFISSKTLFLCLILFLILFWLCFDKARKEFRINRWQKKLNLK
                     HHAQIFHLLYGDVNGFIISQQARKNRDAIEYVYGEIEFLPFTALLSMVNIDHNTIFYD
                     LGSGTGKAVVACAMVYPVHKSIGIELFPNLHQCACERLQKLAAMEGYTESSKKVSFIL
                     GDFLTVDLSEATLIFINSSTLFGATWEALNTRLNSLPQLSTVITTSKTLSSSRFKLVT
                     RAKIQMSWGVVFAFIHKKEN"
     gene            complement(7547..8272)
                     /locus_tag="U6309_001911"
     CDS             complement(7547..8272)
                     /locus_tag="U6309_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505350.1"
                     /translation="MEHYQEDDLARIKELLRHTGEFIAYFEFVEAKMMEWREGIEQQA
                     ERVLHIAQSLQNELTSMQNLISQSGVTHFKISAEKALAQGETHLHSLERTCNQFTQIF
                     QQQQERLNALTENCIDRIEKHTGQATQTLTLQLSKYDVQHFHRIANESCDHVDRVAND
                     AINKSNKMLNMLQFKVGLFAMLITVLTAFVIVIYLNDEMPWEMHHQAMSERQAGKVLL
                     QAWPKLSQKEKAKILNEDGLKPG"
     gene            8857..9900
                     /locus_tag="U6309_001912"
     CDS             8857..9900
                     /locus_tag="U6309_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013102055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505351.1"
                     /translation="MPRARFFKAETNSSHVQQSFINVGGEQFHAAAVALIDYFKRSPR
                     ISDDTLKKILERFYFHFPKYIPSQPYLTPSERMSMLINGARKSEIVECFAYVLRQLAI
                     DEIYAKPLNYRDIFAEFDRDTPKSLLRQPSTPLPASALRALSHVIGTNITLSVVENGK
                     ELRKREVYVGDNLNSAKPGITIQVQQNNRYFPAVKNKADFVYVGQLAVSPPKPIESSN
                     TSGKDTIADMVDLIASDNKKLLQTYKQWHQNFLTMLKLGEITTQRMINLYIKFLPSEK
                     GLVVDSTEFFSRLSEDSDKPIIPDYSGENKDGVNQLLASALAGWISARQVDVDQLFDH
                     LENRPSSVSMSVG"
     gene            complement(9995..10945)
                     /locus_tag="U6309_001913"
     CDS             complement(9995..10945)
                     /locus_tag="U6309_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEN5505352.1"
                     /translation="MIYPNILATIGHTPVVKINRLGKELECELYAKCEFFNPGGSVKD
                     RIGYEMVVKAEKEGRIKPGDTLIEPTSGNTGIGIALAGAVLGYKVIITMPEKMSQEKQ
                     SVLERLGATIYRTPTEAAYDAPDSHISLAKKLQAEIPNSHILDQYANPNNPNAHYFGT
                     AQEIIDDFGKDLHMVVAGVGTGGTITGIARRLKEYNPAIKIIGADPEGSILGGGTEVK
                     SYHVEGIGYDFFPDVLDNTLIDAYIKTNDADSFRTARRLIKEEGLLIGGSCGAAMWAA
                     LQAAKSLSKGQKCLVILPDSIRNYMSKFANDEWMKEMGFL"
     gene            complement(11055..11510)
                     /locus_tag="U6309_001914"
     CDS             complement(11055..11510)
                     /locus_tag="U6309_001914"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505353.1"
                     /translation="MKIFGLNKAILLTFSLLSLLTSQAFSQNNFTGNGRASLEIKAVS
                     IPGKSGLVSCHNSLLAECFITLSIMAQSEGFIIIKNTSANPAFNIRALLPADWTNVSQ
                     PSICEVLNPGEECSVVLKATGDEPPRPRTTMELKGTNTNSAFFDLEVVA"
BASE COUNT         3397 a         2189 c         2430 g         3531 t
ORIGIN      
        1 taattaagtt ctttttgagc ccatcaccaa aatgatgggc tttttttttg aaatggagtc
       61 catatgttga aaaagacaag tttagcccta ttgggattgg ctgcaagtgg ttatgcattg
      121 gcaggaacta tgggacctgt ttgtacgcct ggtaatgtca ctgttccctg tgaagcaaaa
      181 cgttgggatt taggtgtgca ggcactctat ctaaaaccag tttatgatgc cgatatggcc
      241 tatagacaag ttcacgtagc ccccacaaga tatgacgatg atcaaggttg ggattgggga
      301 taccgattgg aaggttctta tcactttaat actggtaatg atgcgactct tacatggatt
      361 cactacagtg attttgcaaa tcctagtggt cttgtcgggc gaattgatat ccctccattg
      421 ccgcctgcat tagcgttccc acggttattt agcgcaatat acaaaaacag atttgagcaa
      481 gtgaacttgg tgatgggtca gcatgctgat tttggcctag ttaaagatat gcgtttctat
      541 ggtggtttac agtacgctga tattgaaatt gacaatacca actattattt tggtgcgttt
      601 cctcagttag gcggagttaa cctgttcgat aataccgatt acaaaggagt aggacctgtt
      661 gtaggaatcg attatgctta tgcactcacc aatgcattga gtgtcacagc aaatggtgct
      721 gcttcagtct tatatggtaa gggtaagtat aacatcgggt atgttgcatt gcctacaggc
      781 tcttctggcg cagtagtgcg atccatctat gcaaacaaaa gaacgatagt tcccagtctg
      841 gaagctaagc ttggattaaa ttatgcttac aatatgaccc aaggagtatt aaatcttgag
      901 ggtggctatc aggtaatgaa ctattttaat gccctacata cacaattcct gcaaaacatg
      961 gcaaacccag taaccaatag tgattatgga ctctatggac catatattgg tgcaaaatat
     1021 attggtaatg cctaaacgaa gtcctttact taaatgcgtt cgtcattgac gaacgcattg
     1081 ctttaaaata ctgcttaaaa atcttgaaaa aaatttttta acaaaaatat aatcacgtta
     1141 atccattcta agtctaacca actaaacccc taatttattg atgtataggc aataaactat
     1201 aaatgcatac aaataaatca atttatttta aattttagcg gagtgatcct atagccagcc
     1261 agtaattgcc gcgctataat ccggtggcta tccgtttagg atgtaaaaat aataaacagg
     1321 agtaccccgc atgaagttta aaaaaattat tttggctctc gcttgtttat catcgccatt
     1381 atatgcggat caagatcaac aactgaaaag tgaaattcaa cgattacagc atcaagcaga
     1441 agatttacaa gcccaactca accgattaga aaaacaatta gcaaatcata aaagctctca
     1501 gcaaaagcac gagcaacggg ctgccacaaa gcctgcagaa ccacagagta agccgacggt
     1561 taaatcaggg gctgcaatag aagaaaaata tcactcatcc aaagtcgaag tgcatgcgcc
     1621 tgatgcccac ccagagtcaa taagctttta tccaacagca ttgatagccg ataaccgagt
     1681 ggttacttat atagcaggga ctcctgtagt aagctcgcca ttccttggcg ataggcctgc
     1741 gtttgatggc tctgattata tagtaaacat ttccagtatt aatagggatg ttcgtttatt
     1801 gcagcagcga cgacgcttat acagagccta ccaaaaaata ggttacccta tccctaatat
     1861 gccaattatt tcattaagtg gtaaaacaga acctgctgcg acatttaaca atccatttcg
     1921 atcaactaat acggatggtg atattacgct aggctcaagt gaactggacg ttgctgctgc
     1981 attgaatgaa aatgtcgaag cttatattgc aattgcctat gatgaaagcc ctcctgcaat
     2041 tggcccgcgg gttaataact cggcatttaa tttgaatatg ggatttgtca acattggtaa
     2101 cttggataaa agcccgcttt attttactgc agggcaagtc tatgttccat tcggccgtta
     2161 ttccagtgca atggtaagtt cccctgtgac aatgaatttg gcaagaacca agacaaggcc
     2221 tgtcattttc ggttataaat ctcaaggtga taccggtcct ttcggtgctg tttatggcta
     2281 cagaagcgat actacattag gtcgctcagg agtcggtggt gttaatctgg ggtatatttt
     2341 cgggtttgat aatgacatca atggcgagat agggggcggc tttattagct ctgttgctga
     2401 tgcaggcggt atgcaaagca caggcgccaa cgtaggaaca acgtttggtg gttttggctc
     2461 cataactaac ggtaatgaaa acgttcgtaa aacaaaagct gctgacgttc atggccacgt
     2521 gggttatgat cgttataatc tgactttgga atgggttggt gccgtacaga gttttagacc
     2581 ccaggacttg agttttaatg gccaaggcgc cagacctcag gctgcgcaag cagagctggg
     2641 tatgactttt atggctttca gcagaccagc ttccatagga gttggttatc agtggacaaa
     2701 agaagcatta gcactaaatc tcccgaaaca acgttatatt ggagtattta atatttccat
     2761 ttggaaagac acggtcgaga gtattgagta caggcacgat attgattatg gacttactca
     2821 gtttgctaat ggcgcagctc ctccaggatt agtcaacctt ccaactttag gtacaggaaa
     2881 atctgccgat actgtttctg cacaaattgg tgtttatttc taaatcatta ccattctgaa
     2941 tagcaagctt gtaacgaatc aatcggttac aagctgatat gtttatagat ctacccgcat
     3001 caggtgcgag gtgtgtcttg ctcaatttaa ctcacctcaa taacagataa agatcttgaa
     3061 ttcgtgtgac actccctctt gttttctcaa cctgtgctat cttttatttt ataactacct
     3121 cttaaaggga tgtaaaaatg catcgggatt gcgtcaggca agaggggctg cacaaactca
     3181 ataatctttt ttgcaaatgg gctgcggtga caagaactct gcaagcaata atattcagaa
     3241 gcagactatc tgtgtcagtt cttgaagatt cagattctgt agcagtaaaa caatccccgc
     3301 catcggtaac gtgcaagatc aagctcagta aattcatgca aacaagttat atttttatat
     3361 tccttaccct gttcctttta gttggattgc aaacttcctt tgcagcaaaa attgtagagt
     3421 tgaatataaa aggaccaatt ggccctgcga cagcagatta tttggaaaga ggtattaaaa
     3481 gcgctcaaga tgctgatttg atagtgattc taatcgatac accaggcggt ttatatgatt
     3541 caacacgaaa tatcattcag ttatttttac tttctgatgt gcccatagtg acttatgtaa
     3601 gtcctactgg agcaagggct gcaagtgcag gaacttattt aatgtacgcg agtacattag
     3661 cggcaatggc gcctggtact caaatggggg ctgcaagtcc tgtcagcctt ggcacaggat
     3721 tcagtgaagg tgaaaaagat gagaaaaaaa aatcaaccat ggaaaataag gtaactcatg
     3781 atgctgtagc aacaataagg tcacttgctc aactgagagg acgcgatctt gattttgctg
     3841 aaaaggcagt gacagaaggc aaatccataa cagcgaatga agccctcagc aagggggtgg
     3901 tcaattatat tgcaaaaaat agagatgatt tattgtttca aatcaatgga ataaaagtca
     3961 gtcaaaataa caagacaata actattaata ccgaaaaccc tgacattcaa gtaatcaatc
     4021 cagattggag aacccgcttt ctatcagtca ttaccaaccc tacagtggct tatttattat
     4081 tgctacttgg aatttatgga attttctttg aattggttaa tccaggttat gttttacctg
     4141 gagtagttgg tgcagtttca atgctttttg cgctgtatgc gctgcagctg ttacctataa
     4201 attatgcagg tttgggcttg attattttag gtattttatt tgtcatagcc gaggcattta
     4261 ccccaagttt tggagcgtta ggtgtaggag gaactgtttc ttttatttta ggttccatta
     4321 tgctgatgaa cacggaacac ctggcttttc agatagcgtg gtcagcaatt tgggctatgg
     4381 cagttctgaa tatattgatt tttgttttgg ttctggggat gttaattaaa tctcgaaacc
     4441 aaaaaatcag acatggcctg gaaacgttgg tgggcgcaaa aggcagggcg ttaggagaca
     4501 ttcatctgga aggtcaggct gtaattaagg gagagatttg gaatgtccat tcaagctcgc
     4561 ctatcgcggc aaataaaagt gttaaagtca cccgagcatc aggattattg ttggaagtgg
     4621 aggaagatca gtcggtttat tagttggtta ttccatattg aatgaaaagg attaaggaga
     4681 gtgagatggg accatttctg gttatattac tggtagcgat aggcttgcta ctggcttcaa
     4741 tgttcaaggt atttcgggaa tatgaacgtg gggtggtttt tatgctgggt cggttttggc
     4801 gtgttaaagg cccgggcttg ataatcatta tcccaattat tcaacaagtg gtgcgtgttg
     4861 atttgcgaac catagtcatg gatgtaccca gtcaagatgt tatctcaaga gataacgttt
     4921 ctgtacgcgt caatgccgtg gtgtattttc gtgttgtcgt tcctgaaaat gcaatcatcc
     4981 aggtagaaaa ttattttgaa gcaaccagcc aactggcgca aaccacattg cgttcagtat
     5041 taggtcaaca tgatttggat gatatgttgg ctgaacgtga gcagctgaat agtgatgtgc
     5101 aaaaaattct tgatgcccaa acagaaagct ggggaatcaa agtttcaaat gttgaaatca
     5161 agaaggtgga tttggatgaa agtatgataa gagcaatagc caaacaggct gaagcggaaa
     5221 gagacaggcg agcgaaagta atccatgcgg agggagagct gcaagcttcg gaaaagttat
     5281 tacaagcctc tcaagtacta gcccagcaac cccaggcaat gcagctaaga tatttgcaaa
     5341 ctttagccac tatagcggtt aataacaatt caacgataat tttccctatg ccaatggaga
     5401 taggtgatat attaacttcc atggccggca agaaaaaata gaccttgaac agtggcataa
     5461 gccccaaata tacctaagtg tatagagtgc tgccattgga taggagaaca tggtgatgag
     5521 attttttatt ccacaacagg ttgtggtatc agtatttttt taaaatgaga gcttttatcg
     5581 aagtttcaat catcttattc tgtataattt cccaaaacaa actttcgttt cattgacttt
     5641 tatgatagaa atctatcttt ttaagaaaag aggaatatcg tgtcaatcaa atcagacaga
     5701 tggatagaga aaatggccct tgagcatggg atgatttctc catttcaggc cggtcaagtg
     5761 cgagaaaacc aaaatggtag gattatttct tatggcgtct ccagttatgg gtatgatgtt
     5821 cgctgttcga atgaattcaa gatttttacg aatattaatt cagcgattgt tgatcccaag
     5881 gcgtttgatg aaaatagctt tgtagatgtt cagtctgatg tctgcattat tccccccaat
     5941 tcgtttgcct tggcaagaac tgtcgaatat ttccgtatac cccgcaatat attaacgata
     6001 tgcttaggta aatccactta cgcgcgttgc gggattatag tgaatgttac tcctctggaa
     6061 ccggaatggg aggggcatgt tactctcgag ttctccaata cgaccacttt gccagccaag
     6121 atttatgcgt atgagggagt tgctcaaatg ctgtttttag aggctaatga ggtttgtgct
     6181 gtttcttatc gtgatcgaaa cggaaagtac cagggacaaa ccggggttac actaccacgc
     6241 acttaggatg aaaaataaaa tttaatcttt atccaaacgt gggaacttgg ctcgcgacga
     6301 attgacccgc tctcgtaatt cctttccagg tttaaagtga ggactgtatt ttgcctctgt
     6361 tactactttt tcacccgttt taggattatg agcatttcgc ggtggtcggt aatgaagaga
     6421 aaaactgcca aagccacgaa tttctattct ttggccatgg ataagtgctt cactcattaa
     6481 ttcaagtatt ctgttgatgc catcggctac ttgcttttca gtaagatgcg tcattcgagc
     6541 agcgatgtgt tcaatgagtt cggatttaat catatactca cctcgttttg tcgcgtattt
     6601 aatagaccga ttgcattctc tattatttta gcttgtctgc ctatgcttct attcgctttg
     6661 tatcttgaaa cacgggtggc cgatactaaa ataaactcca cgagctgagc aactggtcta
     6721 atgtctgcta ccaagacaca tcaggtgctg acaatttccc atttgatttt ataagtatag
     6781 ttcgggtaaa aaattattca atgttttcaa gctggttagt aaaaattagt tttctttctt
     6841 atgaatgaat gcgaaaacca caccccaact catttgtatt ttagcgcgag taactaattt
     6901 aaaacgactg gatgataggg ttttgctagt agtaatgacg gttgaaagtt gtggcaggct
     6961 attgagacgg gtgttaaggg cttcccaggt tgcaccgaat aaagtacttg aattaataaa
     7021 tattaaggtc gcctcagata aatcaacggt tagaaaatca cccaatataa aagagacttt
     7081 tttgctactc tctgtatagc cttccatagc cgccaatttc tggagacgct cgcaagcaca
     7141 ttgatgtaag ttagggaaca attcgatgcc tatactttta tggactggat ataccatcgc
     7201 gcaggcgaca acagccttac cagttccaga gccaagatca taaaaaatag tattatgatc
     7261 aatattgacc attgacagta aagcggtgaa cggtaaaaac tcaatttccc catacacata
     7321 ttctattgca tctcgattct ttcttgcttg ttgggaaata atgaagccat taacatcacc
     7381 gtacaggaga tgaaaaattt gagcatgatg ctttaaattt agcttttttt gccatcggtt
     7441 aatgcgaaac tcttttcttg ccttatcaaa acaaagccaa aaaagaatga gaaacagaat
     7501 gaggcataaa aaaagcgttt ttgacgatat gaaatcaagc acagtattat cctggtttca
     7561 gaccatcttc attcaagatt tttgcctttt ccttttgact taattttggc catgcttgaa
     7621 gaagaacttt accagcctgg cgttcgctca tggcttggtg atgcatttcc caaggcattt
     7681 catcattaag gtaaatgact atcacgaaag cagtgagtac ggtgattaac atggcaaaaa
     7741 gacctacttt gaattgaagc atattaagca ttttattgct tttgtttatg gcatcgtttg
     7801 caactcggtc gacatgatca cagctttcgt tagcgatgcg atgaaagtgt tgaacatcgt
     7861 atttagatag ttgcaaagtc agtgtttgag ttgcttgacc agtatgcttt tcgattctat
     7921 caatgcagtt ttctgttagg gcattgagcc tttcttgttg ttgttggaag atttgggtaa
     7981 attgattgca agttctttct aaagaatgca aatgcgtttc gccttgagcc agggcctttt
     8041 cagcacttat tttaaaatgg gttactcctg attgagaaat aaggttttgc attgaagtta
     8101 attcattttg tagtgattga gcgatatgta gaactcgttc agcctgttgc tcaattccct
     8161 ctcgccattc catcattttc gcttcgacaa actcaaaata ggcaataaat tctccagtat
     8221 gtctcagcaa ctctttaatg cgagccaagt catcttcctg ataatgctcc atgcctctct
     8281 ccttactaaa ttcatagtca gagaatcttc aaatagactt cttgcataat ctgtcctttt
     8341 cattttattg ccgtgttgca aacgtttcta tgtttgcact ttgcctaaaa cgaaataatc
     8401 agtttataca agagggctaa taatatcctt tgatgcatcc tgcctctttg gccgtgaatt
     8461 tagaattatg atgttccata ttatattata tctataactt tattttggaa acggcaattt
     8521 ttgcctaaaa ttctcactca attttcgtta ttagttccat aatttctaga ctggtacagt
     8581 atagtcatgc ctcataaagt tggcattatt tatgcttgaa tgtaatatac tcggcatata
     8641 ctttctttat tattaaaatc cactgagacg agttgtcagt ttgcagaaat aatcgaagct
     8701 caatggcatt atatgaaaat gagcggtggt ttaaaaatga tgtggttaag agaaacgccc
     8761 cgtgttaaaa gtttcagtag actgtaaaaa ctttgttaat atttccttaa tctggattga
     8821 ttagaatgaa gatataagtt gggataggag aacattatgc caagagctag gttctttaaa
     8881 gcagaaacta atagttcgca cgtacagcaa agctttatta atgtgggcgg agagcagttt
     8941 catgcggctg cagttgcttt aattgattat tttaagagat ccccacgcat tagtgatgat
     9001 actttgaaaa aaatactgga gcgtttctac tttcattttc caaaatacat tcccagccaa
     9061 ccttatttga ccccctccga acgcatgagt atgttgatta atggggcacg gaagtcagaa
     9121 attgtcgaat gttttgctta tgtattacgc caattggcta tagatgagat ttatgcaaaa
     9181 cctctcaatt atcgtgatat ctttgctgaa ttcgaccgcg acacgccaaa atcattgtta
     9241 cgtcaaccat ctactcctct cccagccagt gctttgagag ccctttctca tgtaatcggt
     9301 acaaacatca ctttatctgt tgtggaaaat ggcaaggaat tgaggaaacg tgaagtgtat
     9361 gttggggata atctgaatag tgcaaaaccc gggataacca ttcaggtcca gcaaaataac
     9421 aggtatttcc ctgccgtgaa aaataaagct gattttgttt atgtagggca attggcagta
     9481 agccccccca aaccaataga aagctccaat acaagtggga aagacactat cgctgatatg
     9541 gttgatttaa ttgcgtctga taataaaaaa ttattacaga cctataagca atggcatcag
     9601 aactttctaa ccatgcttaa attaggcgaa ataacaaccc agagaatgat caatctttac
     9661 atcaagtttt taccttcaga aaaaggcttg gttgttgact caacagaatt tttttcgagg
     9721 ttatcagaag attctgacaa gccgattatt cctgattatt ccggtgaaaa caaggatgga
     9781 gtcaatcagt tattggcttc tgcgctggcg gggtggatta gtgccagaca ggtcgatgtt
     9841 gatcaattgt ttgatcattt agaaaatcga ccttcttcag tatccatgtc tgttggttaa
     9901 agtgtgttgg caatccggat cattttttca aagcggattg tcaacggtca ttttttgatg
     9961 aaatagcaat acgatagttt tttatggctt tcaattaaag aaagcccatt tctttcatcc
    10021 attcatcatt ggcaaatttg gacatgtaat tacggataga gtcgggtaaa atcaccagac
    10081 atttttgtcc tttgcttaag gatttggctg cttgcaatgc ggcccacatc gctgctccac
    10141 atgagccgcc aattaacagt ccttcttctt taatgaggcg tctggcagta cggaaagaat
    10201 ccgcgtcatt ggtttttatg taggcatcaa tcaacgtgtt atcaagcaca tcggggaaga
    10261 aatcataacc tatcccttca acatgatacg atttaacttc tgtaccgccc cccaggattg
    10321 atccttcagg atctgcacca atgattttga tggcgggatt atactcttta aggcgtctgg
    10381 caatgccagt aattgttcca cctgttccta cgccagcaac aaccatatgc aaatcttttc
    10441 caaaatcgtc aataatttcc tgagctgtac caaaataatg ggcattagga ttattggggt
    10501 ttgcatactg atcgaggata tgggagttag ggatttcagc ttgtaatttt ttagccagag
    10561 aaatatgact gtccggagca tcatacgctg cttctgttgg cgttcgataa atggtggctc
    10621 ccagccgctc aagcacagat tgcttttcct ggctcatttt ttcgggcatg gtaataatca
    10681 ctttgtagcc taatacagcg cctgccaatg ctatccctat cccggtattt ccggaagtcg
    10741 gctcgattaa agtatcgcct ggtttaattc gcccttcctt ttctgccttt actaccattt
    10801 catagccaat acggtctttt acagagccgc caggattaaa aaattcgcat ttggcataca
    10861 gctcacattc cagctctttt ccaagtctgt taatttttac aacgggagta tggcctatag
    10921 tggccaggat gttagggtaa atcatggtag tcctttgtca tggtgaaatg agttgattat
    10981 aagcgtataa ggtaaaatag caaaatagat acgcttgcga atcagctcat ttttcctagg
    11041 ttacaaagta atgtttatgc aacaacttcc aagtcaaaaa aagcagaatt agtgtttgtt
    11101 ccttttaact ccattgttgt tcgcgggcgc gggggctcat ctccagttgc ttttaagacc
    11161 actgagcatt cttcgccagg attcaatacc tcacaaatcg aaggttggct tacatttgtc
    11221 caatcagcag gcagcaaagc tctgatgtta aatgcgggat tagcagacgt gttttttata
    11281 ataataaaac cttcgctttg agccataatg ctcaaagtaa taaagcactc tgctaacagt
    11341 gaattatggc aagatactag cccactcttc cctggtatag atacagcttt aatctccagt
    11401 gatgcccgcc cattaccagt aaaattgttt tgactaaaag cttgagaggt cagtagactt
    11461 aacaacgaga aggtcaaaag aattgccttg ttcagtccaa atattttcat gagctatcct
    11521 tagttaattt aatagaaaaa tctaaaa
//
LOCUS       DASLOE010000093        11435 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.094, whole genome shotgun sequence.
ACCESSION   DASLOE010000093 DASLOE010000000
VERSION     DASLOE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.094"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            396..1769
                     /locus_tag="U6309_001915"
     CDS             396..1769
                     /locus_tag="U6309_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEN5505354.1"
                     /translation="MFQSILIIALMTFSLITQAALPADPIQRCLDIRRLVDITSRQKM
                     AAKEPGVLRFKFHKISASELPLDNPVGNMDEVIQALNNQIENCNNSYGEFQTVTIAGH
                     QFKRQEWCLNTNKKMLTLAKAAQGDFKKFLSSIKTEFDWYKSDGWPENHAGFKKGEFQ
                     FTAYYAPAAVEARSKRGGAFLYPLYSNPGVVSVALESKKYNLKTPLCGVDPLSKVMRG
                     FCLKNADGTYSVAPDREEIEHGALNPKYIIGYLKDPNDSAFLMLQGSGSLLLDGKLFH
                     INYDGANGRPRTMLGRIVQCAQDPTCGGNLDTMERCAKDPKCHDEAKLRCNVSKKIRQ
                     SAASEKLIRQYLNNLTPDKADNLRNRDQSYVFFAKEDGGPYGSENISLTPHVSCATDH
                     RVIPVGMNFIYHCKKATSWCVAQDAGGAIIGAHVDVYKGEGNQAGVEANQLNHTGTLF
                     VALPKRK"
     gene            2145..2435
                     /locus_tag="U6309_001916"
     CDS             2145..2435
                     /locus_tag="U6309_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505355.1"
                     /translation="MSIISNHDITFSSTQKDLIEKQQKNDIKNQHIADAKANKGAETS
                     EKLIVFGAKTTALAACGVALLFPLTAPIIVPIAQVSGAVALVIITRKFIKSS"
     gene            complement(2666..2812)
                     /locus_tag="U6309_001917"
     CDS             complement(2666..2812)
                     /locus_tag="U6309_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505356.1"
                     /translation="MAKQLNGTMKYLSKNRLDITQNQANQLISEEMALGDLRLAPKNS
                     RRPW"
     gene            complement(3405..4814)
                     /locus_tag="U6309_001918"
     CDS             complement(3405..4814)
                     /locus_tag="U6309_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2546 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505357.1"
                     /translation="MEMKMSITVKSLDFDQCISNRKYKESLQTNDGRKVWDANSLFNA
                     NKEILGKNNNGDPIHVFVGSNRQNLKADLINLNAGAATLFIPVAQELCDVMGATFHPL
                     LVPDLICENAAIGDTFHSALQVIKDLNDLNSLDSKSLAELVKSALSGQLNSLHCISDE
                     SKFLMLYSQIQYMAQQYPDEKINFEFYDDKEDILKPLYEIFSRNPDLVPANVTLNIKR
                     YLNGNLMETDFNPILGLGSQQENYQNIVKWIHKQSSSNLRSGNCCQVLEMDNEKIARY
                     CRFGKDETRLKLLDSLENLAKHQVGQKDQKMDDFIKESYEKMGGSKDMDSITLQKSLE
                     EISSAIKVTEAINKVIANYRKEAKSLFSVGMNAKADRIEKALLNVPVEDRGKIFSNDK
                     ASPELIAIRAALASHRYFGKRGNVYYKDEARTVIDENKAATTYNNLRKQFANLRTQSH
                     VDAQVELEHSPEVSRTLKL"
     gene            complement(4991..5326)
                     /locus_tag="U6309_001919"
     CDS             complement(4991..5326)
                     /locus_tag="U6309_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEN5505358.1"
                     /translation="MTISYEEFEKVDLRSGTVVKAEPFLRARKPAYKVWVDFGADIGV
                     LQTSAQITVHYTPEALIGRMVIGCVNLGEKNIAGFLSQFLLLGFSDEKGAICLASVEP
                     HVPNGKKMH"
     gene            complement(5404..6159)
                     /locus_tag="U6309_001920"
     CDS             complement(5404..6159)
                     /locus_tag="U6309_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN5505359.1"
                     /translation="MLLTNRIDLRSYHTESCSHTHDFAQLVLPVSGSMELEVGHYSGV
                     VNDDIGVYIAPNEQHCFAGSQKNLFLVIDATTQNSLRDEAFESNILNLTASTKKLIQF
                     THHYLAHNERDFFTDSLINQLLFHFAASSFSPEPDPVVIRAKQWIDLYFADSVDVSRV
                     AKHCYLSISQLQRRFKQMLGCGIAEYWRMKKLLHAKKLLSQKNCSIEAIAFEVGYENL
                     PAFSRRFSKVFGESASQWRTKALTAKKMREIDK"
     gene            complement(6416..7012)
                     /locus_tag="U6309_001921"
     CDS             complement(6416..7012)
                     /locus_tag="U6309_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505360.1"
                     /translation="MGSTLGKWIGLIAAFLFLNNGFAHASARPIICDQEYALCTSARC
                     IPTPGSAAKAICDCVVEKGNSAGYKTCEERKPVRGRYKVTSLISTFSFEQFTTKRPMN
                     CPEGLAWSNCVDMPCTVDPQNSKRALCICTIESTQAFFTFGGDCNTNTCATGFWSGAT
                     QENSIILRNALMQEMRSKPKELPRACPAKSSQANQGQS"
     gene            7200..7313
                     /locus_tag="U6309_001922"
     CDS             7200..7313
                     /locus_tag="U6309_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015578674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505361.1"
                     /translation="MDGSGYPNGLKGDEITMVARMVAIADVYEAMSTNRSY"
     gene            complement(7443..8216)
                     /locus_tag="U6309_001923"
     CDS             complement(7443..8216)
                     /locus_tag="U6309_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEN5505362.1"
                     /translation="MKRQNTIVRFFHFIFFIQLSYLCYAKNLITDKNLTKEFATLPKH
                     CRPNINPTLPQFIIGYGSLMQEESKKEDSSMVGENFPIYVSGFQRGWIERGTPIGFST
                     IYLGVVPKKDSIINAVYFKLNDPSQIKNYDKRENTYCRIKVPRDDIQALSSINLPEGQ
                     FWIYTTSGKQLAAPSSDYPIVQSYVDIFLSGCFSLEEKYHLKNFARDCIKTTSNWSGH
                     WVNDRIYPRTAFDNIPYVAKIDPLIAVELPQFFKLIKIE"
     gene            complement(8423..9418)
                     /locus_tag="U6309_001924"
     CDS             complement(8423..9418)
                     /locus_tag="U6309_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEN5505363.1"
                     /translation="MQKTARRKGIVFIIISYIILSADEPVVAKLIQLGNNFTIHGRNP
                     ISFCNLLFIGSLMGALTLFFIQYKNFRTFNFKTLTRKDWAWLFTSAFFIGFLTPSLFF
                     FGIMYANIINVILLSTLNAPITLFAAWLIFSEKPNMRLILASLITISGSFVIVLIQQW
                     TATNEKPITQITSTGPLYDFLADTPNSGEICVFLGVVSSTISTLISFHAVTTLPGGIF
                     NTFRMGLGAVFFFFIALALFGWGHFSDLLSPFLWKWMILYGPIIVGMGMFFNYLGLQH
                     IKVADDVIASSLTPLFSIVFSYLILGEAPGVAQIIGGCIIFTGIVLAMREQIRHL"
     gene            complement(9864..>11435)
                     /locus_tag="U6309_001925"
     CDS             complement(9864..>11435)
                     /locus_tag="U6309_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2552 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505364.1"
                     /translation="TQKDWIITSFNQYQFTKEESQKVQQAILNGDLRITDLLNRPFSH
                     YFWSFLTFKWGEILSGGKRLSKQEVLQIASCSSNGQEFARTPAQNRNRLIKGVPLVIG
                     KVVTSMALCMVFCLGLLSTLGAPFGLSLGLAIVLSLCNGIVCLLSRGKAMLDSAGFQP
                     QKQKTFKTLEENIKEKTIFDRGQKLFFGVVTLLACISAGISGYAGLVGLLTFLGAGML
                     ISNPLVLILTIGLSVVAAVSFYSFQAIGIRENYTKFKNLFIEDYQKPYGLLRCVLLGM
                     VSTVFLAVYATLTWFTTVSGVNKLFNALGATPDTLLAHFIAMICTSTTMVVNTFSQVY
                     KVFNPKTYVDLKEKFESIYQPPEENLTRAQKIQHGVINMLKLFALIGDSLAYSVAGGI
                     RSGIHIGETLGETIGAKFALTITMAILSPVILVFVSIAFTWPTVFDRKKSTPCLSEMD
                     KPLIEKMSHSHTSDALQNNTLLPTSEKSPKPNDSNIRQAGLRFFDVGLSEERTHKPNC
                     NPTLESRAVLTSPSP"
BASE COUNT         3673 a         2220 c         2045 g         3497 t
ORIGIN      
        1 ccaataaaaa attatggatt tattgttatg ttgaacacga tgattaatca atcgcgaggt
       61 tcaatcaaac actgaggcta accgatgcta tacgccacat ttgacaataa cttaaaaact
      121 cttttttgga cagtaaagtt tcaaccctaa aaaagaagtg ctttaatcgc aggtaaatta
      181 agggcatcaa ctggtgtatg taaacgcttg gtttgaaccg ctttttaaac agggacaatt
      241 gggattcgac actatttctg tataatgata aggtagttat agggtggctg aacttatgta
      301 aattcatctc tttgatgagt tggccaatag cctatataat tattgaaatc aatggacggg
      361 caatgcctca accatagcta agctaaagga taccaatgtt tcaatccatt ctaataattg
      421 cactaatgac ttttagcttg atcactcaag cagccctccc tgctgatcca atccagcgct
      481 gcctggatat tagaaggctc gtggatatca cctcaagaca gaaaatggca gctaaagaac
      541 ccggggtgtt acgttttaaa tttcataaaa tttcagcatc agaactcccg ttagataacc
      601 ctgtgggtaa tatggatgaa gttatccagg cattaaataa tcagattgaa aactgcaata
      661 atagctatgg tgaattccag acagtaacaa tcgctggcca tcaattcaaa cgccaggaat
      721 ggtgcttaaa taccaacaaa aaaatgctga ctctggcaaa agccgcccag ggtgatttta
      781 aaaaattttt atcgagcatc aaaaccgagt ttgactggta taaaagcgat ggctggccgg
      841 aaaaccacgc aggatttaaa aaaggtgagt ttcaatttac tgcctattac gctccagccg
      901 ctgttgaggc ccgctctaaa cgtggtggag catttctgta tcctctctat agcaaccccg
      961 gggttgttag cgttgcgttg gaaagtaaaa aatataattt gaaaactcct ctttgcgggg
     1021 tggatccgct tagcaaagtg atgcgtggat tttgtctgaa aaatgccgat ggcacctact
     1081 ctgtagcacc agatcgagaa gaaattgagc atggggcttt aaatccaaaa tatataattg
     1141 gctatcttaa agacccaaat gactctgctt ttttaatgct acaaggctct ggttcattac
     1201 tccttgatgg caaattgttt cacatcaatt acgacggtgc caacggcagg ccacgcacga
     1261 tgcttggtcg catagtccaa tgcgctcaag atccaacctg tggtggcaat cttgacacaa
     1321 tggaacgttg cgcaaaagat ccgaaatgcc atgatgaagc taagttacgc tgcaacgtat
     1381 ctaaaaaaat caggcaaagc gctgcatcag aaaagctcat tcggcaatat ctgaacaatc
     1441 ttacccctga taaagcagac aatttacgga atcgagatca aagctatgta ttttttgcca
     1501 aagaagatgg tggcccttat ggttcagaaa atatttctct aaccccccat gtgtcatgtg
     1561 caaccgatca cagggttatt cctgttggaa tgaattttat ctatcattgc aaaaaagcta
     1621 cttcatggtg tgtggctcag gatgcaggcg gcgccattat aggggctcat gttgacgtct
     1681 ataaaggtga gggaaatcaa gcaggtgtgg aagcaaatca gttaaatcat actggaacgt
     1741 tatttgtggc actacctaaa cggaaataga aagaaggcaa tcaatactta tttcgagtaa
     1801 ttacttttat tggctatagt catttagggt gctgttaatc tacaaccgaa taaataagaa
     1861 cgctttttga tttggattac tttgcgcaac gagcaaggaa aatgtggcta agctttttcc
     1921 tatgatctga gaacaaaatg attccattgg agtactgatt aacatcctcc aagctcgcca
     1981 ttaaaaccac atatttttaa tacatattca gcatttatat tatataactt attgaaaaaa
     2041 aaagatatta caaatagtat atatcacata tgattacaat tcattgtcta ttttttaatc
     2101 tttatgatta aatataatta atcggttcgc ttctggagag tattatgtcc atcatatcta
     2161 atcatgacat cacttttagt tccacccaaa aggatttgat cgaaaaacag caaaaaaatg
     2221 acattaaaaa tcaacacatc gcagatgcaa aagcgaataa gggagcagaa acatcagaaa
     2281 aattgattgt ttttggtgcc aaaacaacag ctcttgcggc atgtggtgtc gctctactct
     2341 ttccgctaac agcaccaatc attgtgccca tagcacaagt cagtggtgca gtggcactag
     2401 tcatcattac tcgtaagttt atcaaaagct cctaacagcg cctttaggcg ctagaatgca
     2461 atgggaacgc ttgtattact ttccattgca atgagagcta atttcagaaa ttccatgtat
     2521 ttctattgga gcagctctat caggaaattc agccattaat tttaatgatc cttccataac
     2581 tgaactatat ttactcaaag ttgaaattaa cctatcagaa catttttcca aacgagagat
     2641 tccatcttat ttcatatgta atatttcacc aaggtcttct tgagtttttt ggtgccagac
     2701 gtaaatcacc caaagccatt tcttcagaaa tgagttgatt ggcttggttt tgagtaatat
     2761 ccaacctatt ctttgagaga tatttcattg tgccattaag ttgtttcgcc atgaaacact
     2821 tcactgtatt aaatttgata aatgttcatc aaatatcaaa tcttccgttt aaattaaatc
     2881 gttataaagc cgcgtttcct tgccacctga ttactttcta caaacggagg gaatcacctt
     2941 tcgcgcagta tcaaaaacaa ttgctaacac tatacccctt tgcctgcgtt aaatacgagc
     3001 tctttcttat tgtcatgttt tgaaccattt gattttgtat ggactcacag cttaaaccta
     3061 tctcactcaa ataaaaaaat tgacaatcga tacataaatc tctcagacct ctgctatcaa
     3121 atattacctt attatcacaa agaacattgc ttaaaaatta cagtttaatg gggtctgttt
     3181 acatttcgtt tcgcatagcg aaaatgtgga taattaggtg ccattatgag cttgaatgag
     3241 gcgaatagcg agccctattt aacgaattca agctcataat ggcgccaatt agctgcattt
     3301 gcagccgtga aatgaaatgt aaacagaccc taaacttttg gtagtaataa aacaagccgg
     3361 aactggtgaa caaatccggc tggaatagaa atttatagaa ccatttataa ttttagagtt
     3421 cttgaaacct caggcgaatg ctccagttca acttgggcat caacatggct ttgtgtcctt
     3481 aaattcgcaa attgctttct taaattattg taagtcgtcg ccgctttatt ttcatcaatc
     3541 acagtacgtg cttcatcttt ataatagaca ttaccccgct tcccaaagta tcggtgtgaa
     3601 gccaaagctg ctcttatcgc aattaattcc ggagaggctt tgtcattaga gaaaatttta
     3661 cctcgatctt ccacaggtac gttgagtaat gccttttcta ttctgtctgc tttggcattc
     3721 atgccaacgc taaataagct tttggcttct ttgcgataat ttgcaatgac cttgttaatc
     3781 gcttcggtta cttttattgc actactaatt tcctcgagtg atttttgcaa cgttatagaa
     3841 tccatatctt ttgagccacc cattttttca taagactctt tgataaagtc atccattttt
     3901 tgatcttttt ggcccacttg atgtttcgca agattctcaa gagagtctaa tagttttagc
     3961 cttgtctcat cttttccaaa tcggcagtat cttgcaatct tttcattatc catctctaat
     4021 acttggcaac aattccctga ccttaggttg ctgcttgatt gtttatgaat ccattttaca
     4081 atattctgat aattctcttg ttgagatccc aagcccaaaa ttggattaaa atcagtctcc
     4141 ataagattgc cattcaaata acgtttaata ttcaaagtca cattagcagg tactaaatca
     4201 ggatttctag agaagatctc atacagtggc tttaatatat cttccttgtc atcgtaaaac
     4261 tcaaaattaa tcttctcatc aggatattgt tgagccatat actgtatctg tgaatacagc
     4321 attaaaaatt tactctcatc gcttatgcaa tggagtgaat ttaattggcc actgagcgca
     4381 gattttacca gttctgctag agacttactg tctaatgaat tcaagtcatt caagtcttta
     4441 atcacttgca gagcgctgtg aaaagtatct ccaattgcgg cattctcaca gattaaatca
     4501 ggaacaagta atggatgaaa agttgccccc atgacatcgc acaactcttg agctacgggt
     4561 ataaataaag tagctgctcc tgcatttaaa ttgattaaat ccgctttgag attttgcctg
     4621 ttagaaccaa caaaaacatg gatgggatcg ccattattat ttttaccaag aatttcctta
     4681 ttggcgttaa ataaactatt ggcatcccaa actttcctcc catcatttgt ttgtaatgac
     4741 tctttatatt tcctgtttga gatacattga tcaaaatcca aggatttaac ggttatgctc
     4801 attttcatct ccaagataac aataattatt tcgatatgtt taattatttt gcatgttgtg
     4861 ctgttatttt gaaaacgaat tatatttata actgatatca gtaattttaa tatcaataaa
     4921 tttaatcaat agggctaaaa aaacaaatcg ttagttcaga tttgtgatgt atcaatattt
     4981 cagataggaa tcagtgcatt tttttaccat taggaacatg gggctcaacg gaagccagac
     5041 atatagcgcc cttctcatcg gaaaaaccaa gcaatagaaa ttgagagaga aacccggcaa
     5101 tatttttctc ccctaaattc acgcatccaa tgaccatccg ccctatcaat gcttccggcg
     5161 tataatgcac tgtaatttgc gcagaggttt ggagaacgcc aatatcggca ccaaaatcca
     5221 cccaaacttt ataagcaggt ttcctggcac gcaggaatgg ttctgctttc acaacggtac
     5281 cggaacgcaa atcgactttt tcaaattctt cataagatat cgtcatgttg cacctcaatg
     5341 ttttatatag gctgtatcgt taccaccaaa tgaattcgta gtctatcaat ttaaaaccag
     5401 agcttattta tctatttcac gcattttctt tgctgttaac gctttggttc tccattgaga
     5461 tgcagattca ccgaatactt tactaaaacg gcgactgaat gcgggcaagt tttcataacc
     5521 tacttcaaat gcgattgctt caatggaaca atttttctga gataataatt ttttagcatg
     5581 gagtaatttt ttcatccgcc aatattcggc aataccacag cctaacatct gcttaaaacg
     5641 gcgctgcaat tgactaatgc tcaaataaca atgtttcgct accctgctca catccacaga
     5701 atctgcaaaa taaagatcaa tccattgttt tgctctgata acaaccggat ccggttcggg
     5761 tgaaaaagaa cttgccgcaa agtgaaacaa taattgattg attaaagagt ctgtaaaaaa
     5821 atccctttca ttatgtgcta aataatggtg ggtaaattgg atgagctttt tagtactggc
     5881 ggttaaattg agaatattag attcaaaagc ctcgtcacga agagagtttt gagtagttgc
     5941 atcaatcact aaaaacagat ttttttggct tccggcaaaa caatgttgtt cattaggcgc
     6001 aatgtatact ccgatatcat cattaacgac accggagtag tgacctacct ccagctccat
     6061 agaacccgag acgggcagta ccaattgcgc aaaatcgtga gtgtgactgc aactttcagt
     6121 atggtaagaa cgaagatcaa tacgattagt cagcaacata ttgctcccga gcgaacattt
     6181 acctttaaaa gaaacagatt atatcatact tgatttgaaa cccattgaga gaggtagcca
     6241 aaactttgga ttagcttgag cttttatttc ataaaaccca aagggatagg tcggaactga
     6301 cagaggctta aaatgcctga ccaaagctta ttgaaaatag acctcgtgat tgctcaggat
     6361 ttgctcttgt tatattttgg acataacaga ttctttggat cattataaat tttaatcaac
     6421 tctgcccctg gtttgcttgt gatgatttag cagggcaagc cctgggtagt tccttaggtt
     6481 tcgaacgcat ttcttgcatt aatgcattac gaagaatgat actattctct tgggttgcac
     6541 cagaccaaaa accggtagca caagtattcg tattacaatc accgccaaaa gtaaaaaaag
     6601 cttgagtgct ctctatagta cagatgcaca aagcacgttt tgaattttgt ggatcaacag
     6661 tgcacggcat atcaacacaa ttcgaccaag ctaatccttc tggacaattc ataggtcttt
     6721 tagtagtaaa ttgctcaaat gaaaaagttg aaataagtga tgtcactttg tatctgcctc
     6781 gaacaggctt tctctcttca caagttttat aaccagcact gttacctttt tctactacgc
     6841 aatcacaaat tgctttggcc gcacttccag gtgtaggaat acagcgagca gaagtacaaa
     6901 gcgcatactc ctgatcacat ataattggcc ttgcagaggc gtgtgcaaaa ccattattaa
     6961 gaaataaaaa agcagctatt aatccaatcc attttccaag tgttgagccc attgttgagg
     7021 tccctctgtt cttttgttta tcacagtatc tttatcatat tatcgaattt tcataattca
     7081 aaaaaagcat tggagtttac tatactgaat atattattaa gggaccaaat gagtaagata
     7141 attgaacata tgaattttcc ttggtacatt aaatctgtag ttaccaacat catgaacgca
     7201 tggatggttc tggttacccc aacggactca aaggggatga aatcaccatg gtagcacgca
     7261 tggtggctat tgcggatgtg tatgaagcca tgtcaacgaa tagatcttat tgacatttca
     7321 tgttgcaaat aactatctta aagcatctgg aggaaggtaa agaggttttg tctgatacca
     7381 gttatgttga gtgaatacgt gaaagggggc agatacaaca taaaaagcta tcattgctta
     7441 cttcactcta ttttaattaa tttgaagaat tgtggaagtt ctactgcaat aagaggatca
     7501 atttttgcga cataggggat attatcaaat gcggttcttg gataaattct gtcgttgacc
     7561 caatgacctg accaattgga cgtggtttta atgcaatccc tcgcaaaatt ctttaaatga
     7621 tatttttctt ctaaactaaa gcaacctgat aaaaaaatat caacataaga ttgcacaata
     7681 ggataatcac tggaaggagc agcaagttgt ttcccactcg ttgtataaat ccaaaactgc
     7741 ccttcaggta gattaatgga gctcagggct tgaatgtcat ctcgtggaac tttaattcgg
     7801 caataagtat tttcacgttt gtcgtaattc ttaatttgag atggatcatt taacttaaaa
     7861 taaaccgcat taatgatact gtcttttttg ggtactacac caagataaat ggtactaaat
     7921 ccaatcggtg tgcctcgttc aatccacccc ctttgaaaac ctgatacata aattggaaag
     7981 ttttctccta ccattgagct gtcttctttt ttggattctt cttgcattaa cgatccatag
     8041 ccaatgataa attgtggcaa agttggatta atattaggcc ggcaatgctt aggtagtgta
     8101 gcaaactcct tagttaaatt tttatccgtt attaaatttt tggcataaca taaataactc
     8161 agttgaataa aaaaaataaa atgaaaaaat cttactatag tattttgacg tttcattttt
     8221 atgatcctat taaacaatgc gcccgatacc cgaagagctt ataaccttgc atccatcacg
     8281 tttctatcat aaataatact ctcgttacct atttatagag ttaggataga tgccatatag
     8341 aaaagaaaag cttccaaggt tactctttgt caatttttca tgcatccctt ttggggaaaa
     8401 ggtaaaaata taaacagcta tctcaaagat gccgtatttg ttctctcatg gctaatacta
     8461 ttcccgtaaa aataatacaa cctccgataa tttgagcaac cccgggagct tctccaagaa
     8521 ttaaataaga gaaaacaatg gagaaaaggg gtgtcaaaga ggatgctatc acgtcatctg
     8581 cgactttaat atgttgcagc cccagatagt taaaaaacat ccccatacct acaatgatag
     8641 gcccatataa tatcatccac ttccaaagaa atggtgataa aagatccgaa aaatgccccc
     8701 aaccaaacag tgctaaagca ataaaaaaga aaaaaacagc ccctaatccc atacgaaaag
     8761 tattaaaaat accaccaggt aatgttgtaa cagcatgaaa acttatcaag gtggaaattg
     8821 tacttgagac aacacctaaa aatacgcaaa tttccccaga gtttggtgtg tctgcaagaa
     8881 aatcataaag aggtccagtc gatgtgattt gggtaatagg tttctcgttt gtagctgtcc
     8941 attgctgaat aagaacaatc acaaaactac ctgagatagt gattagggaa gctaaaatta
     9001 gccgcatatt aggtttttca gaaaaaataa gccatgcagc aaataaagtt attggagcat
     9061 taagagttga gagcagaatt acattaataa tatttgcgta cataataccg aaaaaaaata
     9121 gcgagggtgt taaaaaacct ataaaaaaag cgctggtaaa tagccatgcc caatcttttc
     9181 tggttaatgt cttgaagttg aatgttctaa aattcttata ctggataaaa aatagtgtta
     9241 gagctcccat tagactacct ataaagagga gattacaaaa tgagatggga ttacgtccat
     9301 ggatagtgaa gttattacca agttgaatca gtttggcaac gacaggctca tccgcactta
     9361 atataatata actgattatt ataaaaacta ttcccttacg tctggccgtt ttctgcatta
     9421 ttccctgtac ttaaaacttt ccttaacgtc attgtaaaat atttacagtt catggtcacg
     9481 aacctcagga aaggggcttt ccttttgctt ttcagaaaaa gggaaaggcc tgttcctaag
     9541 tttataagtt ataataagac tggttccaaa ggcaacgaca ccagatcttg tcttttgcct
     9601 atcaaagtag ttctgggtca ttttagagaa agcaaaaaac aagattttct ccccctgctc
     9661 cgttaaattc cataatttta gagagcttag acttacaaaa taattgatat tttcagcaaa
     9721 tcaaaaacca tcgttgaatg actctggagc aattacaaga aaagaacctg caaattagtt
     9781 gtttaacaag tatcgattaa atttactctt ttccagattc aaatcaatcg atacttcact
     9841 agttgattca aaaacctcat catttatggc gagggagatg tcaaaacagc tctgctctct
     9901 aaagtagggt tacaattggg cttatgagta cgctcttcgc tcaagcccac atcaaaaaat
     9961 cgtagcccag cttgcctgat atttgaatca ttaggcttgg gtgatttttc tgatgttggt
    10021 aatagagtat tattttgaag tgcatctgaa gtatggctat gactcatttt ttctatcaat
    10081 ggcttgtcca tttctgaaag acatggagtc gatttttttc tatcaaaaac ggtaggccag
    10141 gtaaaagcta tcgatacaaa tactaaaatg accggagaca atattgccat ggtaatggtt
    10201 aatgcaaatt ttgcccctat tgtttctccc aatgtttcac caatatgaat cccgcttcgg
    10261 attccaccgg caacagaata agcgagagaa tcacctatca aagcaaataa tttgagcatg
    10321 ttgataactc catgctgtat cttttgtgct cttgttaggt tttcttccgg tggttgatat
    10381 attgattcaa atttttcttt tagatcaaca taggttttcg ggttaaagac tttatatact
    10441 tgtgaaaaag tattgacaac catcgttgtt gaagtacata tcatggcaat aaaatgtgct
    10501 aaaagagtat caggagtcgc acctaaagca ttgaaaagct tattaacccc tgagaccgtc
    10561 gtaaaccaag ttagcgtcgc ataaacagcc aaaaaaaccg tacttaccat tcctaataaa
    10621 acacatcgca aaagaccgta gggcttttgg taatcttcaa taaaaagatt tttaaatttt
    10681 gtataatttt cgcgtatgcc gatagcttga aacgaataaa aagagaccgc tgcaaccaca
    10741 gacaacccaa ttgtaagtat caaaaccaga gggttagaga ttagcatgcc tgcccccaaa
    10801 aacgtgagaa gtcctacgag gccagcatat ccagaaatgc ctgcagaaat gcacgccaac
    10861 aaggtcacga caccaaaaaa taatttctgg cctctatcga atatcgtttt ttcttttata
    10921 ttttcttcaa gggtcttaaa cgttttttgt ttttgaggct gaaagccggc actatcaagc
    10981 atggctttcc cgcggcttaa taagcaaact attccattac ataaggaaag tactatcgcc
    11041 aagccaaggc taaggccaaa aggcgctcct aaagtactta ataatcccag gcaaaatacc
    11101 atacacagcg ccatggaggt aacgactttg ccaatgacca atggcactcc ttttatcagc
    11161 ctgtttctat tttgagctgg tgttctcgca aattcctggc cattgctaga gcagctagca
    11221 atctgtaaga cttcctgctt agacaatctt tttccacccg aaagtatctc tccccactta
    11281 aaggtaagaa aactccaaaa atagtgagaa aaaggacgat taagaagatc tgttattcga
    11341 agatccccgt ttagaatggc ttgttgaact ttttgagatt cttctttagt aaattgatat
    11401 tggttgaaag aggtaataat ccaatctttt tgagt
//
LOCUS       DASLOE010000094        11435 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.095, whole genome shotgun sequence.
ACCESSION   DASLOE010000094 DASLOE010000000
VERSION     DASLOE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.095"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            180..2801
                     /locus_tag="U6309_001926"
     CDS             180..2801
                     /locus_tag="U6309_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein FimV"
                     /protein_id="HEN5505365.1"
                     /translation="MKKTVLHAAIFSLTMPISLYALGLGEMKVQSSLDQPFSAEIELI
                     DVGADPLASIKVSLADPEQFEQIGFERTAVLSLLRFQVERNPKGAPVVKVSSTERMTE
                     PYMEIVVDLTWPKGQLYKAYTVLLDPPGYQLGITTAQSRPVYYKKKSASYSNEPGVIE
                     KTVITTVEHGPVSKNDSKKKTTYGPTVTNENVWQIAQRYKTSKLILPQVVLAIVGANP
                     EAFKDGNLNGLKVGVRLVIPSTEEIVKVPSDLAIEEVMAHDKAWNEHTSINHVLAPPY
                     INSSIVDVTPKQAKASSIPVIPKFTIGENSNIPTPQQLIPDSTSVSIDASTQQQPVQN
                     NNIVQTTEQNEKIKAEISITTAAVESVREANALLMEQLRILQEQNKKLQEKLDKREKE
                     IELMRTQVQTMLKERKAIASQVSSLPNNEQGMNLWPYIILLIFAAGGAGFAYWYLRQR
                     QQREDISPYKTDSDSSPKPFMPLVEPAKYEPDTQADDKETSVQEKISDSEKEIGVGVS
                     KADWTEYQPKEMDKIEKIDAVESETVEDVTYLGDTEQLRAKEYTELEPEVQTKPEAQT
                     KPEAQTKPEIQTKPEIQTKPEVQAKPEVQAKPEVQAKPEVQAKPEVQAKPEVQAEPEV
                     QAEPEVQAGENQIMFEPVMEPEQLDVNRDDEVDNSQLSEDETQQTEGHQSSEMIEFES
                     GLHELIKDKPKTKITESTDEGDNNGIDFIPLTINDADKTLKYEQEIEKSDKKEDKKSE
                     EFSTKENKFDLDNSDFDMKNLESEPEINQEEKEDHISEITEFDQKSTQEEKTEKGISI
                     SQSSDEATFTKSSNESNQTSNPLKSKKALDILLDLAKTYISMGDIETARSSLEEVIEH
                     GSVSQKEQARQLLDQLK"
     gene            complement(2858..3325)
                     /locus_tag="U6309_001927"
     CDS             complement(2858..3325)
                     /locus_tag="U6309_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HEN5505366.1"
                     /translation="MLYFYLKFIHVLSSTILFGTGIGTASVMIYGHRTKNPIVIAAIS
                     KYVVFADWIFTGTSGILQPLTGFAMVYLAGFSWTSFWIVGSILGYVVAACCWFPVVYL
                     QIKMRDLAIETVKNNTTLPKKYDRYFLYWFILGWPAFLSLIGVFYLMTNKPVF"
     gene            complement(3325..4623)
                     /locus_tag="U6309_001928"
     CDS             complement(3325..4623)
                     /locus_tag="U6309_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEN5505367.1"
                     /translation="MKILVTGASGFIASQFVTDLLIAGHEIICCVRNTKHTQRIFPGA
                     QVIFCDFINDTKPEIWSKRLQGIDVVINCVGILYHPDERIIWNVHYETPKALFDACIN
                     SGVKKIIQISALGIDKVDVSYAASKKAIDDYLLTLSIPSVIVRPSYVYGKGSYGGSSL
                     FRGIAGTPFFTAIPGQGTQKFQPISLNDLSQAIVRLVSTPVTETIILHAVSKKIITLE
                     EIIIKLRSWLGLTKTRIIHIPLTFIRLLTRLGDLLPYSTINTVSYKMLASDNITTQEE
                     TQRFADYVNFTPRDYEEGLYSQPSSVQDHWHAQLYFLKLPLRLGISFVWIWTALCCLF
                     FYPKSDSLSLFAQIGFSSLGQLFLFYASALWDGLLGIAMLFNYRFKLTSILQLITILV
                     YTVIITIKLPYLWLDPFAPIAKNIPLLIAILIGLGLESDK"
     gene            4867..5655
                     /gene="truA"
                     /locus_tag="U6309_001929"
     CDS             4867..5655
                     /gene="truA"
                     /locus_tag="U6309_001929"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN5505368.1"
                     /translation="MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISV
                     VCAGRTDTGVHATNQVIHFDCEKDRSIRAWIHGANTFLPKDICVKWGKEMPENFHARY
                     SAVSRRYRYVIYNGAIRPGLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVEC
                     QSNTPMRNIHQLQVTRHGDLVVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDV
                     LNAKDRKLGAETAPSYGLYLVQVTYPKEFGLLQNNPGPLFLWEK"
     gene            5656..6279
                     /locus_tag="U6309_001930"
     CDS             5656..6279
                     /locus_tag="U6309_001930"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEN5505369.1"
                     /translation="MNPSRIRIKMCGMTRSEDIQYAIDLGVDAIGLIFYPKSARNVSL
                     EKARIIVNNIPPFVDIVAVLVNPEQSFVQLIINEIPVQLLQFHGEESSEFCRQFNKPF
                     IKAIHPKTAIQIQSAVDEFFDASAILLDTPSDKERGGTGLTFDWNIIPENLSKPYILA
                     GGLNESNILEAITMCHPYAVDVCSGIEASPGVKDHLKMSRFIKAIWG"
     gene            6281..7480
                     /gene="trpB"
                     /locus_tag="U6309_001931"
     CDS             6281..7480
                     /gene="trpB"
                     /locus_tag="U6309_001931"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEN5505370.1"
                     /translation="MIKKELPDEFGHFGPYGGMFVADTLVHALKQLEHAYTKYRNDQD
                     FLSELHTELKDYVGRPNPLYHAVHLSKKIGGAQIYLKREDLNHTGAHKINNTIGQALL
                     AKRMGKTRVIAETGAGQHGVATATVAAKFGFQCVVYMGSEDIKRQSSNVYRMKLLGAE
                     VVPVTSGSKTLKDALNEALRDWVSHVDDTFYIIGTVAGPHPYPQMVRDFQAIIGVEAR
                     AQHMEKTGRLPDALVACVGGGSNAIGLFYPFLNDQSVMIYGVEAGGKGIETGEHSASL
                     IAGKPGVLHGNRTYLLCDEYGQVKDTHSVSAGLDYPGVGPEHAYLKDTGRVIYKAIND
                     SEALDAFRLLTHTEGIIPALESSHAVAYAIQLAKTMSKEQSIIVNLSGRGDKDMHTVA
                     AIDGITI"
     gene            7506..8324
                     /locus_tag="U6309_001932"
     CDS             7506..8324
                     /locus_tag="U6309_001932"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEN5505371.1"
                     /translation="MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSG
                     ADVLELGIPFSDPMAEGPVIQRAMERALAHSIHCDDVLNMVRQFRKMDTETPVILMGY
                     LNPIEQYGYDLFAQQAVEAGVDGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSA
                     ERMNFINQHANGYLYYVSLKGVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPE
                     MAAQVAEFADGVIVGAALINEIIEAYEAKKDPLQASGALLSSMRQAIDNIGSMV"
     gene            8321..9976
                     /locus_tag="U6309_001933"
     CDS             8321..9976
                     /locus_tag="U6309_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEN5505372.1"
                     /translation="MTEQTEKRAHFIRQLIIDDLANGKHQGIVTRFPPEPNGYLHVGH
                     AKSICLNFGLAEEFKGKCFLRFDDTNPIKEEEEYVNAIIDDVRWLGFEWCAMTHSSDY
                     YHELYELAIYLIKKDMAYVDSLSMEEIRAFRGTLQEPGRESPYRNRTIEENLDLFARM
                     KAGEFPDGTHVLRAKIDMKSGNLNMRDPVLYRIRHARHQRTGDEWCIYPMYDYAHPIS
                     DALEKITHSLCTLEFQDHRPLYDWLVDNLPLPAKPVQTEFARLNLSHTVTSKRKLREL
                     VEKKIVTGWDDPRLPTLRGMRKRGYPPAAIRQFCEIIGISRSDSVIDMSLLEECVRAE
                     FNKTAKRALCVMDPLKVVIVNYPENKVEQLQAAFYPQNPESESRNLPFSREIYIEKSD
                     FMENPPNKYFRLSPGAEVRLRHAYVIKCQEVIRNDQGEITELHCTYDENTLGKNPEDR
                     KVKGVIHWVSSSHAYPVTIYQYDRLFTDPNPAREEDYFQFLNHNSLQTLQGFCEPAMA
                     EQAEGDVFQFERLGYYCVNSVAEGCVQAFHRVVDLKDTWGKVS"
     gene            9986..11356
                     /locus_tag="U6309_001934"
     CDS             9986..11356
                     /locus_tag="U6309_001934"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEN5505373.1"
                     /translation="MLHLYNSLTRKKQPFVSLRPGKIGMYVCGITVYDHCHLGHARSM
                     VAFDVMVRYLRSQGFDVTYVRNITDIDDKIIARASERGVSIDELTAQYIDAMNNDTHA
                     LNILPPDHEPRATGHIETIIRLIQRLLEKGNAYVSENGDVCYEVDTFPEYGKLSHKDI
                     EGLVSGSRVEIVKEKRSPLDFVLWKKAKPGEPSWSSPWGEGRPGWHIECSAMAMHELG
                     EQFDIHGGGLDLQFPHHENEIAQSEAATGKPFANYWLHVGMLQVNGEKMAKSIGNFYT
                     IADVLKEHHPEVIRYFLLSSHYRSPLNYSEENLLNAKKALIRLYQAVKDVPPQTADSK
                     LDEYWQEQFNQAMNDDFNTPVALSVLFQLAHEVNKSNSPALVHTLKILAGILGFLQKD
                     PESFLQSGLAEEEKLVIEQLIAERLQARAERNWAKADQIRADLLSKGIELEDGATGTT
                     WRRIAE"
BASE COUNT         3743 a         2026 c         2436 g         3230 t
ORIGIN      
        1 tgagtatttg atcacaaatc actgaaaaat catactaaat gataattact ggtcaaaaag
       61 gtatgatctt gccctgggag taatttcgtg ttttcttgaa tagactaacg gataatgata
      121 ctttttactt aggtaattaa atcatcaaca tcttgtatca caggtgcatg gagctctgat
      181 tgaaaaaaac tgttctgcat gcggctatat tctcattgac tatgccaata tctctttatg
      241 cgcttggctt aggggaaatg aaggtacagt cctcattgga tcagcctttt tcagccgaaa
      301 tcgaattaat agatgtagga gcagatcctc ttgccagtat caaggtgagt ttggctgatc
      361 cggagcaatt tgaacaaatt ggatttgagc gaactgcagt cttgtctttg ttacggtttc
      421 aggttgagag aaacccaaaa ggtgcccctg ttgtaaaagt atcgtctaca gaaagaatga
      481 cagaaccata tatggaaatt gtcgttgatc taacctggcc aaaaggtcaa ttatacaagg
      541 cctatacagt attattagac ccgccaggat atcaattggg aattaccact gctcaaagca
      601 ggccagttta ttacaagaag aaatccgcaa gttacagtaa tgagcctggt gttattgaaa
      661 agactgtgat tactacggta gagcatggcc ctgtatcaaa aaatgacagc aaaaagaaaa
      721 cgacttatgg cccaactgta accaatgaaa acgtttggca aattgctcaa agatataaaa
      781 cttcaaaatt gattttgcct caggttgtat tagcaatagt tggtgcgaat cccgaagcat
      841 ttaaggatgg aaatttaaac ggtttaaaag ttggcgttcg gctagttatt ccatcaactg
      901 aagaaattgt aaaagtgcct tctgatttgg ctattgaaga ggtgatggct cacgacaaag
      961 cctggaatga acatacatct attaatcatg tattagctcc gccatacatc aacagttcaa
     1021 tagtggacgt gactccaaaa caggctaaag cttcttcaat cccggtaatt ccaaagttta
     1081 ctattggaga aaactccaac attccgacac ctcaacaatt aatccccgat agcacttcag
     1141 tttcgataga cgcatctacc caacagcaac ctgtacaaaa caacaatata gtacagacaa
     1201 cagaacaaaa tgaaaaaatc aaggcagaaa tttcaattac aacagcagca gttgaatcag
     1261 ttcgggaagc aaatgcatta ttgatggaac aattacgaat tctgcaagag caaaataaaa
     1321 aattgcagga aaagctggat aagcgtgaga aagaaataga gttaatgcgt actcaggttc
     1381 aaaccatgtt gaaggagcgc aaagccattg cgtcccaagt cagttcactt cccaataatg
     1441 aacaagggat gaacctttgg ccgtatatca ttttattgat atttgctgca ggaggagcag
     1501 ggtttgctta ttggtacctc agacaacgcc aacagagaga ggatatttct ccttacaaaa
     1561 cagattctga ttcaagtccg aagccattta tgcctttagt tgagccagcg aaatacgaac
     1621 cagataccca ggctgatgat aaggaaactt ccgtccagga aaaaatatct gacagtgaaa
     1681 aagaaattgg agttggtgtt tctaaagcag attggactga gtatcaacct aaagaaatgg
     1741 ataaaatcga aaaaattgat gcagtagaat ctgaaacagt tgaggatgtg acatatcttg
     1801 gagatacaga acagttaaga gcaaaagaat atacagaatt agaaccagaa gttcagacta
     1861 aaccagaggc ccagactaaa ccagaggccc agactaaacc agagattcag actaaaccag
     1921 agattcagac taaaccagaa gttcaggcta aaccagaagt tcaggctaaa ccagaagttc
     1981 aggctaaacc agaagttcag gctaaaccag aagttcaggc taaaccagag gttcaggctg
     2041 agccagaagt tcaggctgag ccagaagttc aggctggaga aaatcaaatt atgtttgagc
     2101 ccgttatgga gcctgagcaa ttggatgtta acagggatga tgaggttgat aattctcagt
     2161 taagtgaaga tgaaactcag caaactgaag gccatcaaag ttctgagatg atagagtttg
     2221 agtcggggtt acatgaatta atcaaagata agcccaaaac taaaataacg gaatcaacag
     2281 atgaaggaga taataatgga atagatttca ttcctttaac tataaatgat gctgacaaaa
     2341 cattaaagta tgaacaagaa attgagaagt cagacaaaaa ggaagataaa aaatcagaag
     2401 aattttctac aaaggaaaat aagtttgatt tagataattc agactttgat atgaaaaacc
     2461 tcgaatccga acctgaaata aaccaggagg aaaaagaaga ccacatttca gaaataactg
     2521 aatttgacca gaaatctact caggaagaaa aaacagaaaa aggtatttct atatctcaat
     2581 cttctgacga ggcgacattc accaagtcat ccaatgaaag taatcaaaca tccaatccat
     2641 tgaagagtaa gaaggcatta gatattttgt tagatttggc aaaaacctat attagtatgg
     2701 gcgatataga aacagcaaga agttctcttg aggaagtgat cgagcatgga agcgttagcc
     2761 aaaaagaaca ggccaggcag ttgctggatc aattaaagta ataaataaca cagaaattac
     2821 caaacccccc taatctctta atattcacct ttgatgttta aaaaacaggc ttgttagtca
     2881 tcaaataaaa tactccaata agacttaaga atgcaggcca acccagaata aaccaataaa
     2941 ggaaatatcg gtcatatttt tttggtaagg tcgtgttatt ttttacggtc tctatggcta
     3001 aatccctcat tttgatttgc aggtagacga caggaaacca acaacatgcc gcaacgacat
     3061 acccaagaat tgaacccact atccagaaag aagtccaaga aaatcccgca aggtaaacca
     3121 tggcaaaccc tgtgagtggt tgtaatattc cagatgtacc agtgaatatc caatcagcaa
     3181 aaactacata ttttgaaatg gcagcaataa caatcggatt ttttgttcta tggccataaa
     3241 tcatcacact tgctgttcct atgcctgtac caaaaagtat cgttgaactc agcacgtgga
     3301 taaactttaa atagaaatag agcattattt atcagactcc aaacccagac ctattaaaat
     3361 agctattaac aaaggaatgt tcttggctat gggagcaaat ggatctaacc aaaggtaagg
     3421 cagtttgatg gtaataatta ccgtatatac caaaatcgta atcagttgca atatactggt
     3481 caatttaaat cgataattaa acaacatggc tatacctaaa aggccatccc ataaagctga
     3541 agcataaaat aagaataact gccctaagga tgaaaaacct atttgagcaa acaaagataa
     3601 tgagtcactt ttagggtaaa aaaacaaaca acataaggcc gtccaaatcc agacaaagga
     3661 tattccgagt cttaggggaa gtttaagaaa gtataattgc gcatgccaat ggtcttggac
     3721 tgaactcggt tgactataga gaccttcttc atagtctctt ggagtaaagt taacataatc
     3781 agcaaatcgc tgtgtttctt cctgagtagt aatgttatca ctggcaagca ttttatacga
     3841 aacagtattt atagttgaat aggggagcaa atcaccaagt cgggtcaata gtcgaataaa
     3901 tgtcaatgga atatgtatta ttctggtttt ggtcaaccca agccaggaac gcagttttat
     3961 tattatttct tctagcgtga ttattttttt gcttacggca tgcaaaataa tagtttcggt
     4021 tacaggcgta ctcaccaatc gtactattgc ttgagatagg tcatttagag agataggttg
     4081 aaatttttgt gtcccttgcc caggtatagc ggtaaaaaaa ggtgttccag caatccctct
     4141 gaatagagaa gaccctccgt aagagccttt tccatacaca taagagggtc ttactataac
     4201 tgacgggata gagagtgtca gtaagtaatc atctatcgct tttttactcg cagcataact
     4261 cacatcaact ttatcaattc ccaaagcaga tatttgaata atttttttta ccccagagtt
     4321 tatacacgca tcaaaaagtg cttttggagt ttcataatgg acattccata tgattctctc
     4381 atcagggtgg tataaaatcc ctacacaatt aataaccaca tcaatcccct gcaatctttt
     4441 tgaccagatt tcaggttttg tatcattgat aaaatcacaa aagataactt gtgccccagg
     4501 aaaaatcctt tgcgtgtgtt tggtattccg tacacaacaa atgatttcat gtccagcaat
     4561 cagcagatcg gtaacaaact gtgaagcgat aaacccagat gcgcctgtta ccagaatttt
     4621 cataaggatc tcttattttt gattacaatt ataatagaaa tactataacg aatttaactc
     4681 ctactattca aatttcctca ccaggattaa ttttaatcaa caccagtttg gcatatttag
     4741 gttaaatgaa atagtatgag ttcatgcaaa atgataaaac ccatacaggc aaagagttaa
     4801 tactctaaaa attatagaca aaacaaagag ggtgttataa attgaccctt ttatcaggaa
     4861 aattgaatgc gtattgcctt agtggttgaa tacgatggta gccaatatca cggctggcag
     4921 gctcaaacgg gtttgcatac tattcaacaa gctgttgaat ttgcattatc aaaagttgca
     4981 gacagttcta tttctgttgt ctgcgcaggt agaacagata ctggggtcca tgcgaccaat
     5041 caggtgattc attttgattg tgagaaagat cgcagcatac gagcttggat acatggtgct
     5101 aatacctttt tacctaaaga catctgcgtc aaatggggca aggaaatgcc agaaaatttt
     5161 catgctcgtt attctgctgt gagtcgacgg tatcgctatg tcatttataa tggagccata
     5221 cggcctgggt tattacgcgg taatgtgaca tggcaatatc ggcagttgga tcatcgtttg
     5281 atgcaacagg gtggacaatg tcttctgggg gaaaatgatt ttacttcatt ccgctccgtc
     5341 gaatgtcaat ctaatacacc aatgcgtaat attcatcaat tgcaagttac aagacatggt
     5401 gacttggttg ttcttgatat tacagcgaat gcctttttac atcatatggt aagaaatata
     5461 gctggtgtat tgattgctgt tggctcggga aaacaccctg ttggttgggt taaagatgtt
     5521 ttaaatgcaa aggatagaaa attaggcgct gaaactgcac cgtcatatgg cctatatttg
     5581 gtccaggtga cttatcccaa agaatttgga ttgttgcaaa ataatcctgg ccctttattt
     5641 ctttgggaga aataattgaa tccatctcgt ataagaatca aaatgtgcgg aatgacacgt
     5701 tcagaagata ttcaatacgc cattgattta ggggtagacg ccattggctt aatattttat
     5761 cccaaaagtg cgcgtaatgt ctcactggaa aaagccagaa taatagtcaa taatatacct
     5821 ccatttgttg atattgttgc agttttggta aacccggaac aatcttttgt tcaactgatt
     5881 attaatgaaa ttcctgtgca gttattgcaa tttcatggtg aggaatcttc agagttttgc
     5941 agacaattta ataagccgtt tatcaaagcg attcatccta agacagcgat ccaaatccaa
     6001 agtgctgttg atgagttttt tgatgcgagc gctatattgt tggatacgcc atcagacaag
     6061 gaacgaggag gaacaggatt aacttttgac tggaatataa ttcctgaaaa tttatccaag
     6121 ccttacatat tggcaggtgg tttgaatgaa tctaacattt tagaggcaat taccatgtgt
     6181 catccttatg cagtggatgt gtgcagtggt atagaagctt cacccggagt aaaagatcat
     6241 ttgaaaatga gccgatttat aaaggcaata tggggatagt atgattaaaa aagagcttcc
     6301 agatgagttt ggccattttg gcccatatgg cggtatgttt gtagccgata cattagttca
     6361 tgctttaaaa caattagagc atgcctatac caaatatcgt aatgatcagg attttctgtc
     6421 tgagttacac acagaattaa aagattatgt ggggcggccc aatcctctat accatgctgt
     6481 acacttaagt aaaaagatag gcggtgcaca aatttatcta aagcgtgaag atttaaatca
     6541 taccggcgct cataaaatca ataataccat tggacaagcc ttactcgcca aacgcatggg
     6601 gaaaactcga gtgatcgcgg aaacaggcgc aggtcaacat ggcgttgcaa ctgctacagt
     6661 tgccgctaaa tttgggtttc aatgcgttgt gtacatgggg tcagaagaca ttaagcgtca
     6721 atctagtaat gtttatagaa tgaaattgtt aggtgctgaa gtggtgcctg tcacttcagg
     6781 atcaaagacg ttgaaagatg ctttaaatga agcattgaga gattgggtga gtcatgtcga
     6841 cgacacgttt tatattattg gtactgttgc aggtccccat ccttatcctc aaatggtaag
     6901 agattttcaa gcgattattg gtgttgaagc tcgagctcag catatggaaa aaacaggccg
     6961 tttgccagat gctttggtag cttgtgtcgg aggtggatcc aatgcaatag gcttgtttta
     7021 tccctttttg aatgatcagt ctgtcatgat ttatggggtt gaagcaggag gtaaaggcat
     7081 agaaaccggt gagcattcgg catctctgat tgctggaaaa cctggtgtgt tacacggtaa
     7141 tagaacctat ttattgtgtg atgagtatgg acaggtgaaa gacactcatt cagtatcagc
     7201 cggtctggat tatcctggag ttgggcctga acatgcttat ttaaaagata ctggacgggt
     7261 catttataaa gcaataaatg attccgaggc cctggatgcg tttcgcctgt taactcatac
     7321 ggaaggaatt attccagcat tagaatctag ccacgcggta gcctatgcca tacaattggc
     7381 taaaacgatg tcaaaagaac aaagcatcat agtcaatcta tctggtcgtg gtgataaaga
     7441 tatgcataca gtagctgcta ttgacggtat aacaatttaa ttttgataaa ttattcaggt
     7501 acaacatgaa ccgtattgat aagactctgg aaaaattaaa agccaataga aaaaaaatgt
     7561 taagtcctta tattactgca ggcgatccat atcctgagtt aacagtaagt ttaatgcatc
     7621 aattggttaa atcaggggca gatgtattgg agctgggaat acccttttct gaccctatgg
     7681 cagaaggtcc tgttatccaa agagcaatgg aacgagcttt ggcccattca attcattgtg
     7741 atgacgtatt gaatatggtt aggcaatttc gcaaaatgga taccgaaacg cctgtgattc
     7801 ttatgggata tttaaatcct atagaacaat atggctatga tctttttgcc caacaggcag
     7861 tggaggctgg ggttgatgga acaatattag ttgatttgcc tcctgaggaa gctgatggag
     7921 tatcgcgagt ttggcaaaaa catggtttgt acagtattta tttatgttcg ccaacaacct
     7981 cagctgaaag aatgaacttt ataaatcagc atgccaatgg ttatctgtat tatgtttcat
     8041 tgaagggagt gactggttct gatgcgctta agttgccaga attaaaagct caatatctac
     8101 aacgaaaggc acaatcaaaa ttaccgctta tggttgggtt tggaataaaa actccggaaa
     8161 tggctgcaca agttgctgaa tttgctgatg gggtcattgt tggggcggca ctgataaatg
     8221 aaatcattga agcctatgag gccaagaaag atccgctgca agcaagcggg gctctattga
     8281 gttccatgag gcaagctatc gataatattg gaagtatggt atgacagaac aaactgaaaa
     8341 aagagctcac tttattagac aactcattat agatgatttg gcaaatggaa aacatcaggg
     8401 aatcgttact cgttttcctc ctgaaccaaa tggatatctc catgttggac atgccaaatc
     8461 tatttgtctt aattttggtt tggcagaaga gtttaaggga aaatgctttt tacgttttga
     8521 tgacaccaat ccaattaaag aagaagaaga atatgtaaac gcgatcattg atgatgttcg
     8581 ttggttggga tttgaatggt gtgccatgac gcattcttcg gattactatc atgagctgta
     8641 tgaattagcc atttatctta ttaagaaaga tatggcttat gtcgatagcc tgagtatgga
     8701 agaaattcgt gcttttcgcg gtactctaca agaaccaggg cgcgaaagcc cttatagaaa
     8761 tagaacaatc gaagagaatc tggatttgtt cgctcggatg aaagctggtg agtttcctga
     8821 tggaactcat gttttgagag caaaaataga tatgaaatca ggtaatttaa atatgcgtga
     8881 tccggtttta tatcgtatac gtcatgcccg gcatcagcgt acaggagatg aatggtgtat
     8941 ttatcccatg tatgattatg ctcatcctat ttctgatgcc ctggaaaaaa taacacattc
     9001 tttatgtacg ttggaattcc aggatcatag acctttgtat gattggctgg ttgataactt
     9061 acctttgcct gccaaaccag tacaaaccga atttgccaga ttaaatttat cccatactgt
     9121 tacgagcaag agaaaattac gtgaattggt tgaaaaaaaa atcgttacag gatgggatga
     9181 cccacgattg cccacattac gcggtatgcg caaaagggga tacccacctg ctgcaattcg
     9241 gcaattctgt gaaataattg gcatatcaag aagtgattca gtgattgata tgagtttact
     9301 tgaggaatgt gtacgcgctg aatttaataa aactgcaaaa cgggctctct gtgttatgga
     9361 tcccttaaaa gttgtaattg taaattatcc ggaaaataaa gtagaacagc ttcaagcagc
     9421 tttctaccct caaaatcctg agtcagagag taggaattta cccttttccc gtgaaattta
     9481 tattgaaaag tctgatttta tggaaaatcc gcctaataaa tatttccggt tatctcctgg
     9541 agctgaagta cgcttacgtc atgcctatgt tataaagtgc caggaagtta tccgtaatga
     9601 ccaaggcgaa ataacagaat tgcattgcac ttatgatgaa aatactcttg gcaaaaatcc
     9661 ggaggatcgt aaggtgaagg gtgtcattca ttgggtgtct tcatcacatg cctatcctgt
     9721 cactatttat caatacgaca ggttatttac agatcccaat cctgcacggg aagaagatta
     9781 ttttcagttt ctgaaccata actctttaca aaccctccag ggattttgtg agccggcaat
     9841 ggccgagcag gcagaaggag atgtgtttca atttgagcgt ttaggatatt attgcgtcaa
     9901 ttcagtggct gagggctgtg ttcaagcctt tcatcgggta gtggatttga aggacacttg
     9961 gggtaaagtg agttgagagg cagacatgtt acatttatat aattctttga ccagaaaaaa
    10021 acaaccattt gtttccttaa ggcccggaaa gataggtatg tatgtttgcg gaattactgt
    10081 gtatgaccac tgtcacttag ggcatgcccg ttcaatggtt gcctttgatg taatggttcg
    10141 ttatctgcgc tcacaaggat tcgatgtaac ttatgtacgc aatataactg acatagatga
    10201 taaaatcatt gcaagagcct cggagagagg tgtatctatt gatgaattga ccgcacaata
    10261 tattgatgcg atgaataatg atactcatgc attaaatatt ttgccacctg accatgagcc
    10321 aagggcaacc gggcacattg aaactatcat tcgcttgatt caacgtttgc ttgaaaaagg
    10381 taatgcgtat gtcagtgaga atggcgatgt gtgctatgaa gtagatacct ttcccgaata
    10441 tgggaaatta tcgcataagg atattgaagg cttggtttcg ggttctcgcg tagagattgt
    10501 aaaagagaaa cgttcaccgc ttgattttgt gctatggaaa aaagcaaaac cgggtgaacc
    10561 tagttggtct tcaccgtggg gggaagggcg gcctggatgg cacattgagt gttcagctat
    10621 ggctatgcat gaattaggtg aacaatttga tattcatgga ggtggattgg atttgcaatt
    10681 cccacatcat gaaaatgaaa ttgctcaaag tgaagcggct acgggtaaac cttttgctaa
    10741 ttattggctg catgtcggta tgttgcaggt caatggcgaa aaaatggcaa aatcaatagg
    10801 gaacttttac actattgccg atgttttaaa agagcatcat ccggaagtca tccgctattt
    10861 tctattaagt agtcattatc gcagtccttt gaattattca gaagaaaatt tgttaaatgc
    10921 caaaaaagcc cttatcaggc tttatcaagc ggttaaagat gttccgccac aaactgcaga
    10981 tagtaaactg gatgagtatt ggcaagagca atttaatcag gcaatgaatg atgatttcaa
    11041 tactcctgtt gcattatcgg ttcttttcca attggctcat gaggtaaaca aaagtaattc
    11101 acctgcattg gttcataccc tgaaaatcct tgctggtatt cttggatttt tacaaaaaga
    11161 tcccgaatca ttcctgcaat ccggcttggc agaggaagaa aaattggtca tagaacaact
    11221 cattgcagag cgtcttcagg cacgagcgga acgcaattgg gcaaaggcag atcagattag
    11281 agctgattta ttgagcaaag gcatagagct ggaagatggt gcaacaggaa caacatggcg
    11341 gaggattgct gaataattgt tccttctatg cggttctctt gaatcagaat ggacttctat
    11401 cccatactta atataggttg caattacctt ttcag
//
LOCUS       DASLOE010000095        11429 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.096, whole genome shotgun sequence.
ACCESSION   DASLOE010000095 DASLOE010000000
VERSION     DASLOE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11429
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.096"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(127..948)
                     /gene="motD"
                     /locus_tag="U6309_001935"
     CDS             complement(127..948)
                     /gene="motD"
                     /locus_tag="U6309_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HEN5505374.1"
                     /translation="MGNTSKMKSKQKKTEEHQDSHRWVVSYADFITLLFAFFVVMYAI
                     SSVNVSKYKSLSEGMKSAFNQKDQNKATQSTDNLKDGPEEKKTKGTFKDGLDELNKSL
                     SELEDGDFKLNRQEGWVEIDIKAGSLFDSGSADIKPEALIKLMKLAEKIKNLPFTVVV
                     EGYTDNVPIETPQYPSNWELSAARAATVGRILNGFGIANHRILVTGYGDQYPITDNYT
                     EAGRSQNRRVNILITKNRKVDRILNPDLSRLHFSVISNTNSVELNKKDTKIKDKK"
     gene            complement(905..1678)
                     /locus_tag="U6309_001936"
     CDS             complement(905..1678)
                     /locus_tag="U6309_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HEN5505375.1"
                     /translation="MDTLTLIGLLTGFLAIIVGQIFEGGDLNSLLNFPALLIVMGGTL
                     GAVMVQTPLTTFKRAFRILPWVVKPPHHNFEECRTTLIELSRKARQLGLLSLEDRLDV
                     ETNPLIYKGLELLVTGVDKLTLRQVLEAEIDRTEHQDLRAAHVFESMGGYSPTIGILG
                     AVLGLIQVMRNLADPSQLGLGIAVAFVATIYGVGFANLFFLPVANKLKSCISHKVHFD
                     EMVVEGLVAMASGESPNMLQLKLNNYGQHKQDEIKTKEN"
     gene            complement(1688..2404)
                     /locus_tag="U6309_001937"
     CDS             complement(1688..2404)
                     /locus_tag="U6309_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HEN5505376.1"
                     /translation="MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLD
                     DLIQSGMLGLLEAARHYDSSKGASFETYAGIRIRGHMLDEVRRNDWVPRSVYRNSRMI
                     SDAVRILEHKFGREAKDNEIAEELGVSLDDYHDMLQDSISSHLYGFDDLGVTDDVLFD
                     HEGNGSTEPHRNVMRSDMMNRLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSE
                     SRISQILSQATHRIRSRLPE"
     gene            complement(2501..3370)
                     /locus_tag="U6309_001938"
     CDS             complement(2501..3370)
                     /locus_tag="U6309_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEN5505377.1"
                     /translation="MSKLSKGIKDQASGLRSLSRNKPVKVIAVSAGKGGVGKSNISVN
                     LAIALSQLNYSVMLLDADLGLANVDIMLGLHTKYNLSHVLQGICHLSDIILHGPAGVR
                     VIPAASGTEFMTQLTPAEHAGIIDSFNELTDDLDYMIIDTAAGISETVLSFTRSSQEL
                     IVVVCDEPTSLTDAYALIKVMSKRYEWTHFHILANMVRNVKDGRDLFNKLFRVSGQFL
                     DVQLDYLGAIPFDENIHKSVKKQNPILIAYPDSPAAQALRELAESVSNWSFKPSLGGN
                     TSFFLERLVAGQF"
     gene            complement(3357..4496)
                     /gene="flhF"
                     /locus_tag="U6309_001939"
     CDS             complement(3357..4496)
                     /gene="flhF"
                     /locus_tag="U6309_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEN5505378.1"
                     /translation="MKLKRFVAADTRSAMQQIKAAFGPDAVILSSHNIKDGVEIVAAI
                     DFDETVISTQAAVAIAEPPSRTEKYSAQTPLDDMRQEILTLRGMLEAQLRGNVGIHEP
                     LHTVMLQKLLYLGVSPNTATIWARSVKPDLNQQRAWQFILSQMTEQLPIRDSSRIEEG
                     GIYAFVGPTGVGKTTTLAKIAARFALRFGADKLGLVTMDTYRIAAQEQLILYGKILGV
                     KVCIAQDEVSLARVLRQLNDKKLILIDTAGMNPSDERVTKQMTVLSNYLQSIEKVLVL
                     PATSHYQVLIDAIKRYQANHVDQCIITKLDESLAVGGAISAVIESNLALGYLTDGQRV
                     PEDIKIATRHQLVELFAQQELQLFQEARDISKINPNVRRENYVEA"
     gene            complement(4537..6615)
                     /gene="flhA"
                     /locus_tag="U6309_001940"
     CDS             complement(4537..6615)
                     /gene="flhA"
                     /locus_tag="U6309_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEN5505379.1"
                     /translation="MNNVMNYIEYWMRQGFGTPLILIVMISMMMLPLPPFLLDIFFTF
                     NIALSLVVLLAVIYSERPLDFAVFPTVILVATLLRLALNVASTRVVLLNGHNGTDAAG
                     QVIKSFGEVVIGGNYTVGIVVFIILVVINFVVVTKGAGRVSEVSARFTLDALPGKQMA
                     IDADLNAGLINQEQAIKRRTEVAQEADFYGSMDGASKFVRGDAIAGILVLIINIVGGL
                     IIGIFQYNMNFNDAVHNYILLTVGDGLVAQVPSLLLSTAAAIIVTRVSSAQNMGQAVI
                     SQVFAKPRSLVIAAAIIGLIGIIPGMPHIAFILLSAGLAGIAYLFINQEKIKREQQDK
                     EQNEFSQTSDSKQSELSWDDVNPVDVIGLEVGYRLIPMVDNAQGGILLSRIKGVRKKI
                     SQELGFLIPSVHVRDNLDLKPNHYRLSLAGVIMGESMIHPDKWLAINPGQIFGNLEGI
                     PTRDPAFGMDAVWINENQKEKAHTLGYTVVDASTVVATHLSMILEQNSQQLLGYEETQ
                     QLLDKLAISSPKLVKELVPDGLTLGMVSKVLQGLLSEHIPLTDIRTIVETLAEYASKS
                     KDTEYLISQVRIALSRLITQKISGLNEELPIITLKPELEQLLHNTIQNSGHGGVSFEP
                     GMADKIQQALIQFAAQQQAKQESSVLVVQPSIRTVLARFVRTISSDFHVLSYQEIPDN
                     KQIRIIGTVG"
     gene            complement(6612..7760)
                     /gene="flhB"
                     /locus_tag="U6309_001941"
     CDS             complement(6612..7760)
                     /gene="flhB"
                     /locus_tag="U6309_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEN5505380.1"
                     /translation="MADEEQSQEKTEQPSSKRLKESRKKGQVARSKDFNATVILLFTG
                     LGFFIFGKQLSLQIASIMQQAFDFDRDILVTGNNSLENLFQLTKSGLWSVVPLLLVIF
                     ILSLLAPLLMGGWVFSGQSIQPKFSRLNILKGFKRMISLKGFVEMLKAFLKFVLVASA
                     SILVLRSQVPLLLELGKAPLEIAITSGVMILLKSFVLISASLILIAAIDVPFQLYEHS
                     KSIKMTKQELKDEYKETEGKPEVKSAIRRAQQEVARRRMMSEVPKADVILTNPTHYAV
                     ALSYQKKGKKAPVVIAKGKNLVAFQISKVAKEYKIPIISVPALARAIYFSTKLNAEIP
                     RGLYVAVAQVLAYIFQLRDRQRYDYKPEILQNVPIPPELAREAEEEIE"
     gene            complement(7760..8530)
                     /gene="fliR"
                     /locus_tag="U6309_001942"
     CDS             complement(7760..8530)
                     /gene="fliR"
                     /locus_tag="U6309_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEN5505381.1"
                     /translation="MNLDYQTMIRLMSQFIWPMVRIGGLMLTVPVLSSALLPARIKIL
                     FVFTLSCVCFTYVPHTLSFENFNGLYLVYLVQELLLGLLMGFVLQIVFQVFILGGQII
                     SMQAGLGFAVMVDPTSNASVPLISQLYLMVTTLIFLALNGHLAILETLIESFRIMPIG
                     KSSLDPSIVWSTIMFSGWMFKEAVLISIPAILSLLIVSLSFGVMARVAPQLNLFSLGF
                     PITLFMGFVIIKIGLPTVGTEMVESIKHGIQFITGILR"
     gene            complement(8534..8803)
                     /gene="fliQ"
                     /locus_tag="U6309_001943"
     CDS             complement(8534..8803)
                     /gene="fliQ"
                     /locus_tag="U6309_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEN5505382.1"
                     /translation="MTPESVVSLFSDSVYIILLLLSVLILPGLIVGLIISMFQAATQI
                     NETSLSFIPKLFVTFLVLILAGPWLLKTLINYTDTLISNIPYLIG"
     gene            complement(8812..9561)
                     /gene="fliP"
                     /locus_tag="U6309_001944"
     CDS             complement(8812..9561)
                     /gene="fliP"
                     /locus_tag="U6309_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841869.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEN5505383.1"
                     /translation="MKIKSWLLILILLLLLPVAGHTDPLSFPVVTMQPGTNGSQSYSI
                     NLQILIFMTLLTVLPGLLMAMTSFTRIIIVLSILRQAIGMAQTPTNQILIGIALFMTY
                     FVMSPVFNKINETAIQPYMNERIDFSLALSNAQKPLRQFMLNQTRKNDLSLFEKFANN
                     VPKTLDEIPMSVLIPAFITSELKTAFQIGFILFLPFLIIDLVVSSLLMAMGMMMLSPL
                     IISLPFKIMLFVMVDGWTLVLGSLASSFALA"
     gene            complement(9562..9972)
                     /gene="fliO"
                     /locus_tag="U6309_001945"
     CDS             complement(9562..9972)
                     /gene="fliO"
                     /locus_tag="U6309_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEN5505384.1"
                     /translation="MKSKVLYLAFLSLSLISSVVWSSTISPNAISHGELIRIISGLLF
                     VLFIIIFLSWIVKRMHRVSFTSSKGFQTVASIILGPKEKIVLLKIGDRYLLVGVGSYS
                     INLICDFGNQLPEGFDTDQKSSFADVLKSAIGKS"
     gene            complement(9969..10304)
                     /gene="fliN"
                     /locus_tag="U6309_001946"
     CDS             complement(9969..10304)
                     /gene="fliN"
                     /locus_tag="U6309_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEN5505385.1"
                     /translation="MTQVNETSAEQLNQNQGNPLNPDSGNEKLELILDIPVSVTVEIG
                     RTKMTIRNLLQLNQGGIVALDRLAGDPLDVLVNGTLVAHGEVVVVNDKFGVRLTDIVS
                     KAERIKRLK"
     gene            complement(10314..11375)
                     /gene="fliM"
                     /locus_tag="U6309_001947"
     CDS             complement(10314..11375)
                     /gene="fliM"
                     /locus_tag="U6309_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEN5505386.1"
                     /translation="MTEKDVLSQEEIDALLDSVDESIDDETNDEELNSREQPARKKTE
                     DTTSQELNSTFDSDKKVAEEGVKTVNFTGQERIVKGQLPVLDKIYDRAVRLFAADIYH
                     LTARDFEIKQDPLLITKHKEFMKSLPNPSLIGIYKFKPLRGKGIILFDSTFVYDLVDY
                     YFGGNSQFGAQKDKTDFTATELRVMEVVTKKLVTNLIHAWEPIIQLDVTKFNDETNPQ
                     LVNIAEPEEMLLVARFVLNFGKETGAFYFILPYSMLEPIKQQLELGASRPDDEIDPNW
                     INSLKEELMDVELSVSASMAETVSTLGQVMSWKVGDFVPLEINEEVTMDIEGTPSFTA
                     TLGSTNEKRALKIIKIIRY"
BASE COUNT         3601 a         2474 c         1897 g         3457 t
ORIGIN      
        1 cttgagtagc caatttttcc aaagaatcta ttttgttctg taatttattg atgttttttc
       61 gttgatttaa taaataatta cccaagagta aaaaaattat cgcatgcaaa tacagaaaga
      121 tatgtgtcat tttttatcct taattttagt atccttttta tttaactcta ctgaatttgt
      181 attacttata acactaaaat ggagtctact taaatctgga ttgagaattc tgtccacttt
      241 tctattttta gtgataagaa tattcactct tcgattctgg cttcttccag cttcagtgta
      301 attgtccgtt attgggtatt gatcaccata tcctgtaacc aaaatacgat gattggcaat
      361 tccaaaacca tttaaaatgc gaccaacagt agctgctctt gcagctgata attcccaatt
      421 tgaagggtat tggggcgttt caataggcac attatcagtg tatccctcaa caaccactgt
      481 aaaaggtaga ttcttaattt tctccgccag cttcattagt ttaatcaatg cttctggttt
      541 aatatcagca ctaccagaat caaataatga gcctgctttt atatctatct caacccatcc
      601 ctcttgtctg tttaatttga aatcaccatc ttcaagttca gatagtgatt tattcagttc
      661 atccaaacca tctttaaaag ttcccttggt cttcttttct tcaggaccat ctttaaggtt
      721 atctgtcgat tgagtcgctt tattttgatc cttttgatta aatgccgact tcattccttc
      781 agaaagagat ttatattttg aaacatttac tgaagaaata gcatacatca caacaaaaaa
      841 agcaaataat aaggtaatga aatcagcata agatacgacc catcgatgac tatcctgatg
      901 ttcttcagtt ttcttttgtt ttgatttcat cttgcttgtg ttgcccatag ttgtttaact
      961 ttaactgcag catattggga ctctcaccac tcgccatggc gaccaggcct tcaacgacca
     1021 tttcatcaaa atgcacctta tgagaaatac aactttttaa tttattagca acaggtaaaa
     1081 aaaacagatt cgcgaaccca acaccataaa tagttgcaac aaaagcaacc gcaatgccta
     1141 aacctaattg actgggatca gcgagattcc tcatgacttg aattaaaccc aacaccgcac
     1201 ctaaaattcc tatggtgggg ctatagccgc ccatgctttc aaagacatgc gcagcacgca
     1261 aatcctgatg ctccgttcga tctatttctg cttccaaaac ttgtcgtaag gtaagcttgt
     1321 ccactcctgt aaccagtaat tccaacccct tataaattaa aggattggtt tcgacatcca
     1381 aacgatcttc tagggagaga aggccaagtt ggcgagcttt tctagataac tctatgagag
     1441 tcgttctgca ttcttcaaaa ttatgatgag gaggtttcac tacccaaggc aatattctaa
     1501 acgccctttt aaaagtggtt aaaggagttt gcaccataac agcccctaat gttccaccca
     1561 tgacaatcag taaggccgga aaattaagta aggaatttaa atctcccccc tcaaaaattt
     1621 ggcctacgat aatggcgaga aaacctgtca acaatcctat taaagtcaaa gtatccatac
     1681 ttatttttta ttccggtaat cttgatctaa tacggtgcgt tgcttgactt aaaatctgtg
     1741 aaattcgtga ctcactaacc tccagtattt ccccgatttc cttcaaattc aaatcctgct
     1801 cataatacaa agaaagcacc agcctctctt tacgagggag actatcaatc acttgggtta
     1861 atcgattcat catatcactt cgcataacat tcctgtgcgg ttcagttgag ccattccctt
     1921 catgatcaaa taagacatca tcggttactc ccaagtcatc aaaaccatat aaatgactgc
     1981 taatggagtc ttgcaacata tcatgataat catctaatga cacacctagc tcttcagcaa
     2041 tttcattatc cttagcctca cgcccaaact tatgctccaa tatgcgcaca gcatcagaaa
     2101 tcattctgga attacgataa acagaacgcg gcacccaatc gttacgtctt acttcatcaa
     2161 gcatatgtcc cctgatacgt attcctgcat aagtttcaaa ggatgccccc ttggatgaat
     2221 cataatgcct tgctgcttca agcagaccga gcatccctga ttgtattaag tcatccagtt
     2281 gtacactctg cggtaatcgt ccgagcagat gatgtgcaat gcgttttacc aatgtggcat
     2341 gtgtttttac cagagcttcc tgggtttgtt gatttacttt gctgtatgca gccaaagcat
     2401 ccacgatact ctccttacca taacagcaga ttgtagttat tagtaatttg gtaaatctaa
     2461 aacaaacaga gtacagctaa ttttaagtat agcgaataat ttaaaattgt cccgcaacca
     2521 aacgctccaa aaagaaactt gtgtttccac ctaaagacgg tttaaatgac caattactca
     2581 ccgattcagc taattctctc aaggcttgag cagcaggtga gtcgggataa gcgattaaaa
     2641 taggattttg tttttttact gatttatgga tgttttcgtc aaaaggaatc gcgccaaggt
     2701 aatctaactg cacatccaga aactgtcctg agacccgaaa cagtttatta aacaagtcac
     2761 gaccatcctt aacatttcta accatattag ccagaatgtg gaaatgcgtc cactcataac
     2821 gtttgctcat cactttaatt agtgcataag catctgtcaa agaggtaggt tcatcacaga
     2881 caacaacaat caattcttgc gatgaacgag taaaactcaa tacggtctcg gaaatccctg
     2941 ctgcggtatc gataatcata tagtccaaat catcagttag ttcgttaaat gaatcaatga
     3001 ttcccgcatg ttccgcagga gtcaattgcg tcataaactc tgttccagaa gcagcaggaa
     3061 taactctgac gccagcggga ccatgtaaaa taatatcact taaatgacat ataccctgaa
     3121 gaacatgaga taaattgtat ttggtatgca accccagcat aatatccaca tttgccaatc
     3181 ctaaatcggc atccagaagc ataacactat aatttagttg agataacgct atggctaaat
     3241 tgaccgagat attacttttg cctacaccac ctttccctgc cgaaacagca atcactttaa
     3301 caggtttgtt tcgggacaaa ctcctaagac cagaagcctg atcttttata cctttgctaa
     3361 gcttcgacat aattctctct cctaacattc ggatttattt tgctgatgtc ccttgcctcc
     3421 tgaaataact gaagttcttg ttgcgcaaat aactcaacaa gctggtgacg cgtagcaatt
     3481 ttgatatcct cagggactcg ttgtccatcg gttaaataac ccaaagctaa atttgattca
     3541 ataacagcac ttatggctcc acctactgca agagattcat ccaatttcgt aataatgcat
     3601 tgatccacat gatttgcttg gtaacgctta atcgcatcga ttaatacctg gtaatgactg
     3661 gtagctggta ataccaatac tttttctata gattgcagat aattacttaa aacagtcatt
     3721 tgtttggtta cacgttcatc agaaggattc atcccggctg tatcaatcaa aattaatttt
     3781 ttgtcattta attgacgcaa tactcttgcc aaggagactt catcctgcgc gatacatact
     3841 ttaaccccca aaatcttccc atagagaatt aattgttcct gagctgctat tcggtaggta
     3901 tccatagtga ccaaacccaa cttgtcagcg ccaaaacgta aagcaaagcg tgctgcaatt
     3961 tttgctagag ttgttgtctt accaactcca gttggaccaa caaacgcgta gataccacct
     4021 tcttctattc tactagaatc acgaattggc aattgctctg tcatttgtga caaaatgaat
     4081 tgccaggctc tttgttgatt caaatcaggc tttactgagc gagcccaaat tgtcgccgta
     4141 ttgggactaa cacctaaata caagagtttt tgcagcataa ctgtatgtaa aggctcatga
     4201 ataccaacat ttccacgcag ctgtgcttcg agcattcctc tcagtgtcaa aatttcttgc
     4261 cgcatgtcgt ctaatggcgt ttgtgctgaa tatttttctg ttcggcttgg gggttctgct
     4321 atggctacag cagcttgagt tgatatcact gtttcgtcaa aatcaatagc agcaactatt
     4381 tcaacaccat cttttatgtt atgacttgac aaaataacag catcagggcc aaaagcagcc
     4441 tttatttgct gcatggcact gcgagtatct gcagcgacaa agcgttttaa tttcataatg
     4501 cctcctgcct tcaacgcacc tatgcaaccc caaaaactaa cctacagttc caattattct
     4561 tatttgtttg ttatcaggta tttcctgata agacaaaaca tgaaagtcac tagatatagt
     4621 acgcacaaat cgtgccaaaa ccgttcgtat acttggttga actaccaaaa ctgagctctc
     4681 ctgctttgcc tgttgttgtg cagcgaactg gatcagtgct tgttggattt tatctgccat
     4741 tcctggctca aagctaactc caccatgtcc actgttttgt atagtattgt gcaacaattg
     4801 ttccaactct ggttttaatg taataatggg caattcctcg tttaatccac tgattttctg
     4861 agttatcaat cgtgataagg caattctcac ttggctaatc aaatattcag tatcttttga
     4921 ttttgacgca tattccgcca aagtttcgac tatagtgcgt atgtctgtta atggaatatg
     4981 ttctgataat aatccctgca acactttgct aaccatacct aaagtaagtc catcaggtac
     5041 aagctcttta accagtttag gggaacttat agccaactta tccaatagct gctgcgtttc
     5101 ctcatagcca agtaattgct ggctgttttg ttccaggatc atgcttaaat gagtagcaac
     5161 tactgttgat gcatcaacta ctgtatatcc taaagtgtgt gctttctcct tttgattttc
     5221 atttatccac acagcatcca tcccaaaagc cggatctctt gttgggattc cctctaaatt
     5281 accaaatatc tgtccggggt ttattgctaa ccatttatca ggatgaatca ttgattcacc
     5341 cataatcacc ccagccaagc ttaatctgta atgattaggc tttaaatcca ggttgtcacg
     5401 aacatgaacg gatggaatta agaaacccaa ttcttgcgat attttctttc ggacaccttt
     5461 aattcgtgaa agtaatattc caccctgagc attatcgacc atagggatca agcggtaacc
     5521 tacttccaaa ccaatgacat cgacaggatt aacatcatcc catgataact cactctgttt
     5581 agagtcactg gtttgtgaaa attcattttg ctctttatcc tgctgttcgc gcttaatttt
     5641 ttcctgattg ataaataaat aagcaatgcc ggctaaccct gctgacaata gaataaatgc
     5701 aatatgaggc ataccaggaa ttatacctat tagaccaata atagctgctg caatgacaag
     5761 tgatcttggt tttgcaaata cttgagaaat aactgcctgc cccatattct gagcgctaga
     5821 aactcgggtc acaatgattg ccgctgctgt agacaataaa agggagggaa cttgagcgac
     5881 caaaccatcc ccgactgtca ataaaatata attatgaact gcgtcattaa aattcatatt
     5941 gtattgaaat atgcctatta ttaatccacc aacgatgtta atgattaaaa ccaaaattcc
     6001 tgctatggca tcccctcgga cgaatttact ggcaccatcc attgaaccgt aaaaatccgc
     6061 ttcctgagct acttctgttc gacgcttgat tgcctgttcc tgattgatta gacctgcatt
     6121 gagatcagca tctatagcca tttgctttcc gggtaaagca tccaaagtaa accgcgcact
     6181 tacttcagat actctccccg ccccttttgt cactacaaca aagttaataa cgacaaggat
     6241 aataaatacc actataccta ctgtgtaatt acctccaatc accacttcac caaaagattt
     6301 tatgacctga ccggcagcat ctgtaccatt atgaccattg agtaagacta ctcgtgtaga
     6361 agctacattg agggctaatc ttaacaaagt tgccacaagt atcactgtgg gaaacaccgc
     6421 aaaatccagg ggtctttcac tataaataac tgccaacaaa acaactaaag aaagagcgat
     6481 attaaaagta aaaaatatat ccagtaggaa aggaggcaga ggtaacatca tcatgctgat
     6541 catgacaatc agtattaatg gagtaccaaa cccttggcgc atccaatatt ctatataatt
     6601 cataacattg ttcattcaat ctcctcctct gcttcgcgag ccagttcagg tggaataggg
     6661 acattttgta aaatttcagg cttataatca taacgttgcc tgtcacgtag ctgaaaaata
     6721 taggccagca cctgagccac agcaacatat aaccctctgg gaatttctgc attcagtttt
     6781 gttgaaaaat aaatagcgcg agctaacgca ggaactgaaa taatagggat cttatattct
     6841 ttagcaacct tggatatctg aaaagcaacc aggtttttac ccttggcaat caccactgga
     6901 gccttttttc ctttcttttg gtaactaaga gcaactgcat agtgcgtagg attagtcaat
     6961 ataacgtcgg ctttaggtac ttcactcatc atccgtcgtc ttgcaacctc ctgctgtgcg
     7021 cgacgtatag cgctttttac ctcaggtttg ccttccgtct ctttgtattc atcttttaat
     7081 tcctgtttag tcatcttaat cgatttacta tgttcatata attggaatgg aacatctatt
     7141 gccgcaatta atatcagact cgcagaaatc agcacaaaag attttaaaag gatcattacc
     7201 ccactagtga ttgctatttc taatggtgct ttccccaatt caagcaacaa gggtacttgc
     7261 gatctaagca ctagaataga tgcgctcgcc accaggacaa attttaaaaa agccttaagc
     7321 atctcaacaa atcctttgag agaaatcatg cgcttaaacc ctttaagtat atttaatcga
     7381 gaaaattttg gctgtataga ctgtccacta aaaacccatc cccccataag caatggtgct
     7441 agcaaagata aaataaaaat aacgagcaat agtggaacaa cagaccaaag cccggatttt
     7501 gttaattgaa acaaattttc taaagaatta ttaccggtaa caagaatgtc acgatcgaaa
     7561 tcaaacgcct gttgcattat actggcaatt tgcagtgata attgtttccc aaaaataaag
     7621 aaaccaagtc cggtaaataa taaaatgacc gttgcattaa aatctttgga acgtgcaacc
     7681 tgcccttttt ttcgtgattc ttttaatcgc ttggaagaag gctgttctgt tttttcttgt
     7741 gattgttctt catcggccat cagcgcaata ttcctgttat aaattggatt ccatgcttaa
     7801 tgctctctac catttctgtc ccgacagtgg gcaaacctat tttaatgatg acaaacccca
     7861 taaatagcgt aatcggaaaa cctaaagaga aaaggtttaa ttgcggagca acccgagcca
     7921 taactccaaa agacaagctt acaataagca aagataaaat cgcaggaatt gaaatcaata
     7981 ccgcttcttt gaacatccaa cctgaaaaca taatcgtact ccagacaatt gatggatcca
     8041 gagaagattt accgattggc attattctga aactctcgat tagagtttca agaatagcta
     8101 aatggccatt taatgccaaa aatattaatg tggtcaccat taaatacaat tgactgatta
     8161 aaggcacact ggcattgctg gttggatcaa ccattaccgc aaatcccaaa cccgcctgca
     8221 tggatataat ttgcccccca agaatgaata cttgaaatac tatttgcaac acaaatccca
     8281 ttaacaaacc caataataat tcctgtacaa gatagacaag ataaagacca ttaaaatttt
     8341 cgaaagacaa ggtatgaggc acataggtaa aacacacaca gcttaaagta aaaacaaaga
     8401 gaattttaat gcgtgccggc aatagtgctg acgataaaac cggcaccgta agcatcaaac
     8461 caccaatacg caccatgggc caaataaatt ggctcattaa gcgaatcata gtttgataat
     8521 cgagattcat tttttatcca atcaaataag gaatattgct tatcaaagta tctgtataat
     8581 ttatcaatgt ctttaataac caaggccctg ctaaaattag aaccaaaaag gtaacaaata
     8641 acttgggtat aaaacttaaa cttgtctcat taatttgagt cgcagcctga aacatcgata
     8701 taattagtcc aacaattaat cccggtaata tcaacactga cagtaatagc agaatgatat
     8761 aaacactatc actaaacaac gaaacgactg attcaggggt catatagcct cctaggcaag
     8821 agcaaaactt gaagcaagtg aacccaaaac caaagtccat ccatcaacca tcacaaacaa
     8881 catgatttta aaaggtaatg aaataatcaa gggtgaaagc atcatcatcc ccatagccat
     8941 caaaaggctg gacacaacaa gatcaataat taaaaagggc aaaaaaagaa taaagcctat
     9001 ttgaaatgca gtttttaact cgctagtaat gaaagccgga atgagaacac tcataggaat
     9061 ctcatctaaa gtttttggta cattgttagc aaatttttca aataaagata aatcattttt
     9121 tcttgtttgg tttagcataa actgacgaag cggcttttgc gcattggata atgccagaga
     9181 aaaatcaatc ctttcattca tatagggctg tattgctgtt tcattaattt tgttgaatac
     9241 aggcgacata acaaaataag tcataaatag agcaatgcct attaatattt gattggtagg
     9301 cgtctgagcc atccctattg cctgccgtaa aattgacaga acaataatta tccttgtaaa
     9361 tgacgtcatt gccatcagca accctggcaa tacggtcaat aaagtcataa aaattaaaat
     9421 ttgcagattg atgctataag attgcgaacc attagttcca ggttgcattg taacaacggg
     9481 aaaggataaa gggtctgtgt gacctgcaac gggtaataaa agaagtaata tcaaaatcag
     9541 taaccaactt ttaattttca tttatgactt ccctattgct gatttcagca catcggcaaa
     9601 agacgatttt tgatcagtat caaacccttc aggaagttga ttgccaaaat cacatatgag
     9661 attaatggaa tatgacccta cacctactaa tagatatctg tccccaattt tcagcaatac
     9721 aatcttttcc tttggaccta aaataatact tgcaacagtt tggaatcctt tagatgaagt
     9781 aaagcttact ctatgcatac gtttcactat ccaagataaa aaaataataa tgaacaatac
     9841 aaataagagt cccgaaatga ttctaatcag ttcgccatgg ctgatcgcgt ttgggcttat
     9901 agtagatgac caaacgaccg aactgattag agaaagactc aaaaatgcca gatagagtac
     9961 tttgcttttc atttgagacg ttttattcgt tcagccttgc taacgatatc agttaatctg
    10021 acaccaaact tatcgttaac tacaacgact tcaccatgtg cgactaaagt accattgact
    10081 agcacatcaa ggggatcacc agccagccta tcaagagcta cgattcctcc ctgatttaat
    10141 tgcagtaaat tacgaatagt cattttggta cgaccgattt cgacagttac tgagacagga
    10201 atatctaaaa ttaactccag tttctcattg ccagagtctg gattcagtgg attcccttga
    10261 ttttgattta attgctctgc gctggtttcg ttcacttgag tcatccttat cccctaataa
    10321 cgtattattt taataatttt caaagctcgc ttttcattgg tactgcctaa ggttgcagta
    10381 aagcttggtg tcccttctat gtccatggtt acttcttcat ttatttccaa aggcacaaaa
    10441 tctcctactt tccatgacat tacctgaccc agagtactga ctgtttccgc cattgaagca
    10501 ctaactgaca attcaacatc catgagctct tctttcagag aattaatcca gttaggatca
    10561 atttcatcat caggtcttga tgcccctagc tccaattgct gcttgatagg ttcaagcatg
    10621 gagtatggta aaataaagta aaaagcccct gtttccttac caaaattcaa gacgaaccgc
    10681 gccacaagca acatttcttc tggctcggca atattaacta attgaggatt tgtttcgtca
    10741 ttgaattttg ttacatccag ttgaattatt ggttcccagg catgaattaa attagttacc
    10801 agttttttag ttacgacctc cataacacga agttcggtag cagtaaaatc tgttttatcc
    10861 ttttgagcac caaactggct atttccgcca aaataataat cgaccaggtc atacacaaag
    10921 gtactatcaa acaaaataat tcctttacca cgaagaggct tgaatttgta gatacctatc
    10981 aggctaggat taggtaaact cttcataaat tccttatgct ttgtaataag tagcggatct
    11041 tgtttaattt caaaatctct agccgttaaa tgataaatat ctgctgcaaa cagacgaact
    11101 gccctgtcat aaattttgtc taataccgga agctgtcctt taacaatacg ctcttgtccg
    11161 gtaaaattaa ctgttttgac tccttcttcg gctacttttt tatccgaatc aaaagtggag
    11221 tttaattcct ggctggtagt atcttctgtt tttttacgag ctggctgctc ccttgaattc
    11281 aattcttcat cattcgtttc atcatcaatt gactcatcca ctgaatccag tagtgcatcg
    11341 atctcttctt gtgataaaac atctttctca gtcataagca atccattcgc atctcaacca
    11401 gattttcaaa gttattttaa aattgaagt
//
LOCUS       DASLOE010000096        11313 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.097, whole genome shotgun sequence.
ACCESSION   DASLOE010000096 DASLOE010000000
VERSION     DASLOE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11313
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.097"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(82..1935)
                     /locus_tag="U6309_001948"
     CDS             complement(82..1935)
                     /locus_tag="U6309_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN5505387.1"
                     /translation="MSIVSLNGASLILAGNCILDRADLQIQPQDRIALVGRNGAGKST
                     LLKILQGELVLDSGQIQRSSGLRISGLVQEVPGAEGESVYHFLVKSLGETGEVLSQFH
                     EFTKQGDMDKLAKCQQRMDNLNAWHLLPEIETMASRLGIDIHEQMSNLSGGMKRRVLL
                     GAALLAKPDLLLLDEPTNHLDVEAIEWLENYLKSFSGAVMVVTHDREFLSQIATSILE
                     IDRGKLYLHHCNYETYLDRRESIRLSEQKQNDLFDKRLAEEEAWIRTGIKARRTRNEG
                     RVRALKAMREQYKARRNQLGTVKSLNLDVSRSGALVVEANKVSYAINDKPIIKDFSLL
                     MTRGDKLGIIGPNGCGKTTLVRLLLGEIKPDAGTIKLGTGLQVAYFDQLRRHLLEDQT
                     VMFNVGEGADYVTINGKQKHVASYLKEFLFSSDRFNQPVSSLSGGERNRLLLAKLFAK
                     PVNLLVMDEPTNDLDIETLELLEAVLADYPGTLILISHDRAFINQVVTSVLVYEGNGK
                     FNEFVGGYDDYKKHQLTRNEQQTKSPVSKRSSPEKAKNLSFNEQRELSKLPQQIEAVE
                     KKIEALHSQMASPEFYQQDAKIISEVTQLLAKEEALLNQYFARWEELEEKK"
     gene            complement(1996..2487)
                     /locus_tag="U6309_001949"
     CDS             complement(1996..2487)
                     /locus_tag="U6309_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HEN5505388.1"
                     /translation="MSDDQYARTGRLMFLFVWIIFFIGLFLFFYYYDKPESTIFVASR
                     TEYILSADNEGHYWIKGKINEYPVEFLVDTGATLVAIPQGLAKDLKITGRYPVTIETA
                     NGKVTGFLTRLQHLSFGEFHLQNVKAVIIPENDDNTVLLGMNVLSQFHMIQQGKQLIL
                     KRQ"
     gene            complement(2490..2876)
                     /locus_tag="U6309_001950"
     CDS             complement(2490..2876)
                     /locus_tag="U6309_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEN5505389.1"
                     /translation="MQPINTKLAPKAIGTYSQAIRSGEIVFLSGQIPLDPETMQICSD
                     DIKLQITQVLENLSAVCEEAGGSLANIVKLNVYLTDLSHFPLINEAMSRYFSEPYPAR
                     AAIEVSALPKGAKVEMDGILVLSASA"
     gene            complement(2876..4999)
                     /gene="spoT"
                     /locus_tag="U6309_001951"
     CDS             complement(2876..4999)
                     /gene="spoT"
                     /locus_tag="U6309_001951"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HEN5505390.1"
                     /translation="MSYFKELDEELKCYLEQAQIEKCYQAYLVAEKAHHGQMRRSGEP
                     YITHPVAAALILARMRLDYQTIMATLLHDVVEDTSISKEELVNQFGEEVTALVDGVTK
                     LTKIKFESKAEAQAENFRKMVLAMVKDIRVIIVKLADRLHNMRTLGSMPAAKRRRIAI
                     ETLEIYAPIANRLGMHSIYIGLEDLGFQALYPMRYRAIKSAVEKSRGNRRELTQTIEK
                     DLQHALSQLNIPYEQVFGRQKHLYSIYRKMKQKKASFTEITDVFAFRIITEDIDSCYR
                     ILGALHCTYKPVPQRFKDYIGIPKANGYQSLHTTLFGPFGVPLEVQIRTREMDKVADN
                     GVAAHWIYKSSGLEVNEAQLRAREWVQSLLEMQRSTGSSLEFIENVKIDLFPDEVYVF
                     TPKGHIMELPKGATPVDFAYTVHSGVGNSCVAAKVNRRLVPLSIPLSNGQTVEIITAP
                     GAHPNPAWLNFVVTGKARSNIRHFLKSQQHAESIVLGKRLLEQSLAELSSDYAKVPPE
                     TLQALLSDLHYKTVDDLLYAIGIGNQMAMVIAKRLVVSQDANESDKGNKKPLAIKGTE
                     GMVVHFGECCQPIPGDNIVGKFQQGRGIIVHASDCTTLKTTRSNPEQFIALRWDEQVQ
                     GEYWVDITVDVANERGVLAALATAISEADSNIGNINVDPRDGRHNAVTFSISVRDRTH
                     LARVMRRLRANKVVMRLYRKKQGEI"
     gene            complement(5167..5370)
                     /gene="rpoZ"
                     /locus_tag="U6309_001952"
     CDS             complement(5167..5370)
                     /gene="rpoZ"
                     /locus_tag="U6309_001952"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN5505391.1"
                     /translation="MARVTVEDCLEHVKNRFELVMVATKRARQIAVRGDQPMVEWEND
                     KPTVVALREIAEGYVTADILDED"
     gene            complement(5421..6050)
                     /gene="gmk"
                     /locus_tag="U6309_001953"
     CDS             complement(5421..6050)
                     /gene="gmk"
                     /locus_tag="U6309_001953"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEN5505392.1"
                     /translation="MVSDNSGNLYIVAAPSGGGKTSLVKKLIEMVGEIEVSVSHTTRP
                     MRPGEQEGVDYFFIDEEQFISMVNEGAFIEHAKVFNHWYGTSVAQINKRLQFGIDVIL
                     DIDWQGAEQIRHAYPDAVSVFIIPPSLDALKERLMNRRQDKDHVISERMTKAQDELGH
                     YPEFDYLIVNDDFEKAAMELQSIVIANRLRIEKQVNKQAKLLSFLLSSQ"
     gene            complement(6056..6922)
                     /locus_tag="U6309_001954"
     CDS             complement(6056..6922)
                     /locus_tag="U6309_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEN5505393.1"
                     /translation="MIHSMTAFSRIQKQFDVGDFCWEIRSVNHRYLDVSFRLPEPFRF
                     LEAELRSLLRDKIHRGKLECHLKYKDTSCDNRSMLINIGTINALLDLSSKLSTSHHLA
                     NDMTVSQVLSWPGVVEANPLDIEELSQIVLELFQKSIDKLCEVRAGEGRSLKAYVEDR
                     LQALRQEVMKSKSIVVSMSAKTKDKLLTRLHELQMDVPEARVEQEIALLLAKLDVSEE
                     LDRLETHIAEVSRTLNAEKVTGRRLDFLMQELNREANTLSSKSDSAELTLCAVEMKVL
                     IEQMREQIQNFE"
     gene            7225..7932
                     /gene="rph"
                     /locus_tag="U6309_001955"
     CDS             7225..7932
                     /gene="rph"
                     /locus_tag="U6309_001955"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEN5505394.1"
                     /translation="MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIV
                     EGVPRFLKGKNQGWITAEYGMLPRATHSRTEREASKGKQGGRTLEIQRLIGRSLRACI
                     DLKVLGENTITLDCDVIQADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPAFNYVA
                     AVSVGIYRGQPVLDLDYAEDVLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSML
                     SLAEIGIPQLIEIQKNA"
     gene            complement(8261..9295)
                     /locus_tag="U6309_001956"
     CDS             complement(8261..9295)
                     /locus_tag="U6309_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEN5505395.1"
                     /translation="MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEH
                     KDVIKIIYDIMNDRQRKEYEEFFETDFSFEIANLARFRVNAFNQSRGAAAVFRTIPSE
                     ILSMEDLGLPPIFKEMASFSRGLVLVTGPTGSGKSTTLAAVIDYINSSRYEHILTVED
                     PIEFVHQSKKCLVNQREVHKDTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAA
                     ETGHLVFGTLHTNSATKTINRIIDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGR
                     VAALEIMMCTGAIRNLIREDKIAQMYSSIQTGQAKGMQTLDQHLTELVNKNIISKHTA
                     HEAALNKSLF"
     gene            9459..10151
                     /locus_tag="U6309_001957"
     CDS             9459..10151
                     /locus_tag="U6309_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEN5505396.1"
                     /translation="MSISQNIKKVRELITQKELSSNRQPNSVLLLAVSKEQSPELIRE
                     AFEQGISHFGENYFQEAHEKILALKDLPICWHFIGPIQSNKTKGIAKYFNWVHSIDRN
                     KTAKLLNQFRSEQLPPLNVCLQINLAGETTKSGIPPEHAIDLALEVKQLPNLQLRGLM
                     TIPPQQSNMQTQYDLFLKLNQLKEFINKTLHLKMDTLSMGMSDDLVPAIKAGSTIVRI
                     GRAIFGERKGKL"
     gene            10151..10939
                     /locus_tag="U6309_001958"
     CDS             10151..10939
                     /locus_tag="U6309_001958"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEN5505397.1"
                     /translation="MNISFIGYGNMAKAIARNLQKQNKYSLSAASPSLSAGISKENIR
                     THYDNKEVAKDADVIILAVKPAQMREVVNEINPFISSGCLLISVTAGLSLTWFAKYCK
                     KGQAVVRTMPNTPAIVGFAATSMIANEFVNEKQKKWAEQLFSSIGITDWVKDEEDMDT
                     LTALSGSGPAYVFLFLEAMINAGVQLGLEEATAKHFAIQTFAGSLQLAQHSQLSLTEL
                     RKKVTSPGGTTAAALEILHGDFDELIFSAMKAAKQKAHELGKVE"
BASE COUNT         3456 a         2410 c         1970 g         3477 t
ORIGIN      
        1 tttaaatcct atttttaatt ttatttaaag atatcttttt attgtttttc acttaaactt
       61 atcaattaac caaatacttt tttatttttt ctcttccagc tcttcccaac gagcaaaata
      121 ttgatttaac aatgcctctt ccttggctag cagctgagtt acctctgaaa taatcttggc
      181 atcttgctga taaaattcag gtgaggccat ttgtgaatgc agtgcttcaa tttttttctc
      241 tacggcttca atctgttgcg gcaacttcga taactcacgt tgttcattaa aagataaatt
      301 tttagctttc tctggactgc tccgttttga aactggagat ttcgtttgct gctcatttcg
      361 tgttaattga tgttttttat aatcgtcata accaccaaca aattcattaa acttaccatt
      421 tccttcataa accaacacac tggttactac ttgattaata aacgctctat catgactgat
      481 tagtattaaa gtaccaggat aatcagctaa tacagcttca agaagttcca gagtttctat
      541 atccaaatca ttagttggtt catccattac gagtaaatta accggtttgg caaaaagttt
      601 tgccagtaat aatctgtttc tttcccctcc agacaaggaa gatactggtt gattaaatcg
      661 atcagaggaa aataaaaact ctttcaaata actggcaaca tgtttttgct taccattaat
      721 tgttacataa tcagcccctt cccccacatt aaacatgact gtttgatctt caagcaaatg
      781 tcggcgtaac tgatcaaaat aggctacctg aagccctgtc cctaatttta tagttccagc
      841 atcaggttta atttctccca gaagtaatcg aactaaagtg gttttgccac aaccattggg
      901 gccgataatc ccaagcttgt ctcctcttgt cattaataaa gaaaaatctt ttataatggg
      961 cttgtcgtta attgcataac tcactttgtt tgcttctact acaagagcac cagaacggct
     1021 tacatccaaa tttaaggatt tcactgttcc taactgatta cgacgtgctt tgtactgctc
     1081 tctcattgcc tttaaggccc tgactctgcc ctcgttacgg gtacgtcttg ccttgatacc
     1141 tgtacgtatc caggcttctt cttctgcaag ccgtttgtca aacaaatcat tttgtttttg
     1201 ttcgctaagc cttatcgatt cacgcctgtc aaggtaagtt tcataattac aatggtgcag
     1261 atacaatttt ccacgatcga tttcaaggat gcttgttgct atctggctta agaattccct
     1321 gtcgtgagtt actaccatta cagcaccaga aaaggatttt aaataattct ctagccattc
     1381 aattgcttcg acatccaagt ggttggtagg ttcatccagt aacaataaat caggtttggc
     1441 aagcagtgcc gcacctaata atacgcgtcg tttcataccg cctgagagat tagacatctg
     1501 ctcatgaata tctattccca atcggcttgc catcgtttca atttcaggta ataaatgcca
     1561 tgcattcaag ttgtccattc gctgctgaca cttggccaat ttgtccatat ctccttgttt
     1621 cgtaaactca tgaaactggg acaatacttc ccctgtttca cctaagcttt taacaagaaa
     1681 atggtacact gactctccct cagcacctgg gacttcctga actaaaccag aaatacgcaa
     1741 accggatgag cgttgaattt gtccactatc aagcaccaac tccccttgta gtattttcag
     1801 caatgttgat tttccagcgc cattgcgtcc aacaagagca attctatcct gtggctgaat
     1861 ctgtaaatct gcacggtcca gaatacaatt gccagccaag attaaagagg caccgttaag
     1921 agaaactata ctcatcaaaa ccctctattt aaataaatct gtttgttctc attttattga
     1981 aaacatacag tcagttcact gtctttttaa tatcaattgt ttgccctgct gaatcatatg
     2041 gaattgtgat aggacattca ttcctaataa caccgtatta tcatcatttt ccgggattat
     2101 gaccgctttg acattctgta agtgaaactc tccaaatgac aaatgttgca aacgagttaa
     2161 aaatccagtt accttgccat tggcggtttc tatagtaaca ggatatcgcc cggtaatttt
     2221 taaatcctta gccaggcctt gcggtattgc taccaaagta gcgccagtat ccactaaaaa
     2281 ctctacagga tattcattaa tcttaccttt tatccaataa tgtccttcat tatcagcgct
     2341 taatatgtac tcggtacgac tagctacaaa aatggtgctt tctggcttat cgtaatagta
     2401 aaagaataaa aacaacccta taaaaaatat aatccaaaca aataagaaca tcagacgccc
     2461 tgtgcgagcg tattgatcat cactcatgat tatgcggatg cagacaaaac cagaatccca
     2521 tccatttcaa ccttggcacc ctttggtaat gcggagactt cgattgccgc tcttgcagga
     2581 taaggctctg aaaaatatcg actcatcgct tcgttaatta agggaaaatg acttaaatca
     2641 gtgagataga cgtttaattt gacaatattg gccagactac caccagcctc ttcacataca
     2701 gctgataaat tttccaggac ttgagtaatt tgtaatttaa tatcatcgct gcagatttgc
     2761 attgtttcag gatctaatgg gatctgccct gaaagaaaaa cgatctcacc acttcttatc
     2821 gcttgactgt acgtgcctat cgcttttggc gccaattttg tattgattgg ttgcattaaa
     2881 tctccccttg tttttttcga tataaccgca taactacttt atttgccctt aatcgacgca
     2941 tgactcgagc taaatgggtt ctgtcacgaa cactgatgga aaaagtgacc gcattatgac
     3001 gaccatctct gggatccaca ttaatattcc ctatattaga gtctgcttct gaaatagcag
     3061 ttgctaaagc agccaataca cctctttcgt tggctacatc tacagtaatg tctacccaat
     3121 attcaccctg aacctgctca tcccaacgca aagcaataaa ctgttccgga ttactacgag
     3181 ttgtttttag tgtagtgcag tcactggcat gaacaataat gcctctgcct tgttgaaatt
     3241 taccaactat attatctcca ggtattggtt ggcagcattc accaaaatgc acaaccatcc
     3301 cttcagtgcc tttaatagcc aaaggttttt tattgccctt atccgattca ttggcgtctt
     3361 gcgaaacaac cagccgcttg gcaataacca ttgccatctg attacctatt cctatggcat
     3421 acaataagtc atcaaccgtt ttatagtgta aatcgctgag caatgcctgt aatgtttccg
     3481 gaggcacttt ggcgtaatca ctggataatt cagccaagga ttgctccaat aatcgtttgc
     3541 ctagaacaat agactcagcg tgttgttggc tttttagaaa atgacgaata tttgatcttg
     3601 cctttccagt cacaacaaaa ttcagccaag ccgggttggg gtgtgctccc ggggctgtaa
     3661 taatctctac ggtttgacca ttggataaag gaatgcttaa aggaaccaaa cgcctgttca
     3721 ctttcgctgc aacacaacta tttcccactc cagagtgtac cgtgtatgca aaatctacag
     3781 gagtggctcc tttcggcaac tccataatgt gccctttagg agtaaaaaca tatacttcat
     3841 caggaaataa atctatttta acgttttcga taaattcaag agaactgcct gtgctccttt
     3901 gcatttctag caaactttga acccactctc tggcacgaag ttgagcttca ttgacttcca
     3961 atccggaaga tttatagatc cagtgcgctg ctacaccatt atcagctact ttatccatct
     4021 ctcttgtgcg tatttgaacc tcaagaggaa caccaaaagg cccaaacaaa gtggtatgca
     4081 atgactgata gccattggct tttggaattc cgatataatc tttaaatcgt tgtgggacag
     4141 gtttataagt acagtgcaaa gctcccaaaa ttctataaca ggagtcaatg tcttctgtaa
     4201 tgatgcgaaa tgcgaacaca tccgtaattt ctgtaaagga tgcttttttc tgcttcattt
     4261 ttctataaat actatataaa tgcttttgcc tgccaaatac ttgttcataa ggaatgttca
     4321 attgcgataa ggcgtgttgc aaatctttct ctatagtctg tgtcaactct cttctatttc
     4381 cccgagattt ttcaacagca gacttaattg ccctgtaacg cataggatat aaagcttgaa
     4441 aacccaaatc ctcaaggccg atataaatag aatgcatacc caaacgatta gcaatagggg
     4501 cataaatctc gagcgtttca attgcaattc gtctgcgctt ggctgcgggc attgacccta
     4561 aagtgcgcat attatgtaag cgatcagcca atttaacgat aataacgcga atgtccttaa
     4621 ccatagccaa aaccatcttg cgaaaatttt ccgcttgagc ttcggccttt gattcaaatt
     4681 taattttggt gagcttggtg acgccatcaa caagagcagt gacttcttca ccaaattgat
     4741 taaccagttc ttctttactt atagaggtat cttcaacgac atcatgaagc agagtagcca
     4801 tgatagtttg atagtccagc ctcattcttg ccagaatgag ggctgctgct acagggtgtg
     4861 taatataagg ctcacctgaa cgacgcattt gtccatggtg tgctttttca gccaccaaat
     4921 aagcttgata acatttctct atttgtgcct gctcaaggta gcatttaagc tcttcatcga
     4981 gctctttaaa gtagctcacg cagtactccc tagtatccaa catggctatt tatacctcac
     5041 attactcaaa atacggtttt atgcctcgcc cttgttccct ttgctttgct taaataccat
     5101 aacttgatta ccaagaggta tattttagaa tggctaatat tcccaaggca aagtcaataa
     5161 ctcatttcaa tcttcatcca aaatgtccgc tgtaacataa ccttcggcaa tttccctaag
     5221 tgctactaca gtaggcttat cattttccca ttcaaccata ggttgatcgc cacgaactgc
     5281 aatttgcctt gctcgtttgg tagcaaccat aaccagctca aaccggtttt taacatgttc
     5341 taaacaatct tcaactgtta ctcgtgccat aaaatccacc tctaggttat tagaacaaat
     5401 actaactcaa atcttttatt ttactgcgat gacaagagga aagaaagcaa tttggcttgt
     5461 ttatttacct gtttttcaat tcgaagccta ttggcaatta ctatcgactg taattccatt
     5521 gccgcctttt caaaatcatc attaacaatc agataatcaa attcaggata atggccaagt
     5581 tcatcctgtg ctttggtcat tctctcacta atcacatgat ctttatcttg tcgcctgttc
     5641 atcaagcgtt cttttaatgc gtctaaagaa ggaggaatga taaatacact cactgcatcc
     5701 ggataggcat gtctaatttg ctccgctcct tgccaatcaa tatcaagaat aacgtcaatt
     5761 ccaaattgta accgtttgtt aatttgagca actgaggtac cataccaatg attaaatact
     5821 ttggcatgct caataaacgc cccctcattc accatagaaa taaattgttc ttcatcaata
     5881 aaaaaataat ccactccttc ttgttctcca ggtctcatag gtctggtcgt atgagaaact
     5941 gacacttcaa tctcccccac catttcaatg agttttttca ccaaacttgt tttccctcct
     6001 ccggagggtg ctgcaacaat atataaattt cctgaattat cactaaccat atcatttact
     6061 caaaattctg aatttgttca cgcatctgct caatcaatac tttcatttca acagcacata
     6121 aagttaactc tgctgaatcc gattttgagc ttagggtatt cgcctctcta tttaattctt
     6181 gcattaaaaa atccagcctt ctacccgtca ccttttctgc attcaaagtg cggcttactt
     6241 ccgctatatg agtctctaac ctgtccaact cttcactcac atcaagtttg gctaatagca
     6301 aggcaatttc ttgctctaca cgcgcctctg gaacgtccat ttgcaattca tgcaaacgcg
     6361 taagtaattt atctttagtc ttggcactca ttgaaaccac aatgcttttg gatttcatta
     6421 cttcttggcg caaagcctgc aatcggtctt caacatatgc ctttaatgac ctgccttccc
     6481 cagctctgac ttcacacaat ttatcgatac ttttctgaaa taactccaaa acaatttgag
     6541 acaattcctc aatatccagc ggattagcct ccacaacacc tggccatgaa agcacttgac
     6601 taacagtcat atcattagcc agatgatgag aggtagataa tttactgctt aaatcaagta
     6661 atgcattaat cgtacctata tttataagca tagaccgatt atcacaactt gtgtccttgt
     6721 actttagatg gcactccagt ttacctcgat gtatcttatc gcgaagcaag gatcttaact
     6781 ctgcttcaag aaatcgaaag ggctccggca aacgaaaaga gacatcgaga tagcgatggt
     6841 taacagacct gatttcccaa caaaaatcac ctacatcaaa ctgcttttgt attcgagaaa
     6901 aagccgtcat actatgaatc ataaagaata cctacttttt aaccttgcgc gaatatacct
     6961 gaaattaaaa gaattgcaca gggattgccc atcaattaat tgcatcaaac ttctgtatca
     7021 ggtcctgaaa gtcaggccgt aaaccaaact tgaaacttaa aacaaactgt ttctaacgca
     7081 aagaatggca acagatcgaa tggcttgatt aaaaatattt atttaacaaa aaagagaaaa
     7141 gaatgtacaa atagcgcatg tatttagggt gcaactcaag tattaaagag ttatacttgg
     7201 gcattttaca gaggagtatc tctaatgcgt cccagtaatc gtgaacatga tcaacttcgt
     7261 cccgttacaa taacacgtaa ttttaccaat tatgccgaag gctctgtatt agtcgaattt
     7321 ggtcagacaa aagtgatttg taacgcatca atcgtcgaag gagttccccg gtttttaaaa
     7381 ggtaaaaacc aagggtggat aacagctgaa tatggcatgc tcccacgtgc aactcatagc
     7441 cgtaccgaaa gagaagccag taaaggtaag caaggcggta gaacattgga aattcaacgt
     7501 ctcatagggc gctcattgag agcatgtatc gatttaaagg tattgggcga aaatactatt
     7561 actcttgatt gtgatgttat tcaagcggat gggggaacca gaacagcagc tataactggc
     7621 agctgtgtgg ctatgcgaga tgccattcac tggatggttc aacgtgaaaa aataaagaag
     7681 atgcctgctt ttaattatgt agctgccgta tctgtaggca tttacagggg tcaacccgtg
     7741 ctggatttgg attatgcaga agatgttctt gctgaaacag atatgaatgt ggttatgaat
     7801 gaacaaggac attttattga agttcaaggc actgctgaag ataactcctt taaccgagaa
     7861 caactgaaca gcatgttgtc attagctgaa attggtattc ctcaattgat tgaaatccaa
     7921 aaaaatgcct gagaacaata gcctggcata aacagttgcc agctatagaa attaatactt
     7981 caaacctgaa cagaagatct ccgcaggaat gctttgagct aactgaagag atctcattgc
     8041 agttattagc attgctatct tcaaagtttt ttcaggagtt ctggaacttg cctgtgctca
     8101 attcctgtaa atacgaacac cgtattgaaa tctatctgga cttgcgcccc taatctcttt
     8161 gttaaataat gtttcaaatt tagtcattta atttatataa acttcctcat taaagacatt
     8221 ttttgtctcg accttggggt ttttcgcaag ccctgtgtat ttaaaataat gacttgttca
     8281 gggcagcctc atgagcggtg tgtttcgaaa taatattttt attaaccaat tcagtcagat
     8341 gttgatccaa tgtttgcatt cctttggctt gaccagtttg aattgacgaa tacatttgcg
     8401 ctatcttatc ctcacgaatt aaattacgaa tagccccggt acacatcata atttcaagcg
     8461 ctgcaacccg accaccttta ttctttttta ataaactttg agcaacgaca gcttgtaatg
     8521 attcagaaag catagagcgt accatcgatt tttcttcagc gggaaataca tcaattattc
     8581 tgtttattgt ttttgttgcg gaattggtat gcaatgttcc aaaaaccaaa tgcccggttt
     8641 ctgctgcagt cattgccaag cgtatagttt ccaaatcgcg taattcaccc accaatataa
     8701 tatccggatc ttcacgtaga gctgatctca aagcggcatt aaaactcaat gtgtctttgt
     8761 gtacttcgcg ttggttgacc agacattttt tgctttgatg aacaaactca atgggatctt
     8821 caactgtcaa aatatgctca taacgacttg aattaatata atcgatgact gctgcaagcg
     8881 tggtactttt tcctgatcca gttgggccag tcaccagaac cagacccctg gaaaaactgg
     8941 ccatttcttt aaaaattggg ggcaatccta aatcttccat gctaagaatt tcagaaggaa
     9001 tagttctgaa aacagcagcg gcaccgcgtg attgattgaa agcattaacc ctgaaccgtg
     9061 ccagattggc tatttcaaag gaaaaatctg tttcaaaaaa ttcttcatat tcttttcttt
     9121 gcctgtcatt cattatgtca taaataatct taatgacgtc tttatgctct aatggaggta
     9181 aattaatttt gcgtaaatca ccatcaacac gaatcatagg aggcatgcca gctgaaatat
     9241 gcaaatctga tgacttgttt ttaactgaaa atgccaacaa ttctgctata tccatactct
     9301 ataatccctg ccaatttttt cattatatat tgtagattaa ctcatgaaat gattgccagc
     9361 aaacatggat atctttttgt agtaccatta cacttcatgc ataattaata cttattttta
     9421 taataaagcg ccttatcttt tgattaacca ggattaatat gagtattagt cagaacataa
     9481 aaaaagtaag agaactgata acccagaaag aattatccag taacaggcaa ccgaacagcg
     9541 tcttattatt ggccgttagt aaggaacaaa gtcccgaatt aatcagagag gcattcgaac
     9601 aagggataag tcactttggt gaaaactatt ttcaggaggc ccatgaaaaa atactggcat
     9661 taaaagatct gcctatctgt tggcatttta taggacctat tcaaagtaat aaaaccaaag
     9721 gaatagcgaa atattttaat tgggtccaca gcattgacag aaataaaaca gccaaacttc
     9781 ttaaccagtt cagatctgag caactaccac cgctcaatgt ctgtttacaa attaatttgg
     9841 ctggagaaac tacaaaatca ggcattcctc cagaacatgc catcgacctt gctctcgaag
     9901 taaagcaatt gcctaatttg caactaaggg gactgatgac aatccctcct cagcaaagca
     9961 acatgcaaac acagtacgat ttattcctta aactcaatca actcaaggaa ttcatcaata
    10021 agacacttca cttaaaaatg gatacgttat caatgggtat gagtgatgat ttggttccgg
    10081 caattaaagc aggttcgacc atagttcgaa ttggacgagc aatttttggc gaacgaaaag
    10141 gtaaattatg atgaatatca gttttattgg ttatggaaat atggcgaaag caattgcccg
    10201 caacttacaa aagcaaaata aatactcttt gtcagctgcc tcaccatcct tatctgcagg
    10261 cattagtaaa gaaaatattc gcacccatta cgataataaa gaagtggcta aagacgcaga
    10321 cgtcattata ttggcagtaa aacccgcaca aatgcgtgag gtagtcaatg agattaaccc
    10381 ttttatttca tcaggttgcc ttctaatctc agtgacagcg ggattgtccc tgacctggtt
    10441 tgccaaatat tgtaaaaaag gacaagcggt cgtaagaacc atgcctaata ccccggctat
    10501 agttggattt gcagcaacat caatgattgc aaatgaattt gtaaacgaga aacaaaaaaa
    10561 atgggcagaa caattatttt caagcatcgg gattactgat tgggttaaag atgaagaaga
    10621 catggacacc ctgacagccc tttctggcag tggccctgct tacgtatttt tgtttcttga
    10681 agcgatgatt aatgcagggg tccaattggg cctggaagaa gcgactgcca aacactttgc
    10741 catacagaca tttgcaggat cgcttcaatt agctcaacat agccagttaa gtttgactga
    10801 actgagaaaa aaagtaactt ctccgggagg tacaacagcc gcagctctgg aaatcctgca
    10861 tggagatttc gatgaactca ttttttctgc tatgaaagct gcgaaacaaa aagctcatga
    10921 attaggaaaa gttgaataac tcttttgagt taagaaaaat tctatcgcaa gaagtactct
    10981 attgacttct tttattttaa tgtcagtcag gatttacaaa aaccccaagg tcaaggcaaa
    11041 aaatgttttt aatgagggaa ttaagataaa ctaaacgact gaattaaaaa tactttttaa
    11101 ggaagatgtt gggaacattc tgttagtatt aattcttgaa ctgttcaagc aaacaatcat
    11161 tgtggaacaa ggtttgctcg cgttatgata gaaggttaat aaactgctta agcaattcat
    11221 ttctaaacca tatgaataaa tttgctggct ttattaatga atacaatcta aagaggtaaa
    11281 acaatggctg gtttaactgc tgtcgggtat ttt
//
LOCUS       DASLOE010000097        11283 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.098, whole genome shotgun sequence.
ACCESSION   DASLOE010000097 DASLOE010000000
VERSION     DASLOE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.098"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            39..530
                     /locus_tag="U6309_001959"
     CDS             39..530
                     /locus_tag="U6309_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN5505398.1"
                     /translation="MSKGPYQSFANRLISALKDRGYTASRSPNGICIKTLAEFTGASE
                     QICRRYIRGDALPDYEKVKQLAFHLQVNPGWLLFGEDENATTKKNEVDEKLLHYILKQ
                     SHHLYPISQGSNDDYADFVLGLIKEVKAIDTSENNLLKIIDLAIGSISSYEEKRKKHS
                     HAV"
     gene            520..1131
                     /locus_tag="U6309_001960"
     CDS             520..1131
                     /locus_tag="U6309_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgJ"
                     /protein_id="HEN5505399.1"
                     /translation="MQFKKLVDMDVTAVEVKLHPKAKEFLFEHFVTIRRVFSDVLGQV
                     ETDYASIALINQAGQIFFISSNPAIEQNLIEQSLWLFDGCYQPEFLSQDQPKLWSELP
                     HIGCTESIVKYKQIKPRLITGISIPTEYDSYKAIFSFGLKRINPYIQNKSSIHCEKLL
                     AMGKFALRRIQEYLIFPDKQPCMTTKPKLTLIINNQVPHEHTP"
     gene            1115..1633
                     /gene="def"
                     /locus_tag="U6309_001961"
     CDS             1115..1633
                     /gene="def"
                     /locus_tag="U6309_001961"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN5505400.1"
                     /translation="MNTLLDKNNPILRQTADPISESEFGSSWLKELIKTMFGIMADKG
                     AVGVAAPQIGISKRVIVFGTDYTKRRKPEYPIPDTALINPSLKILSQEIQTGYEGCLN
                     CGELMGEVPRAMEIEYSGFDIDGNRITKKASGLEARILQHEIDHLDGFLFLDRVEDQD
                     SLTTLSAMQSKG"
     gene            1638..2930
                     /locus_tag="U6309_001962"
     CDS             1638..2930
                     /locus_tag="U6309_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5505401.1"
                     /translation="MKHQTRIILAGTLGNLIESFDMAICGLLSVYIAKYLIGDVSKGL
                     FLVFLTFFAGYLARPIGAMIMGLLSDIYGRKIILAGSILTMGISTTLIGFIPPHSTIG
                     TFSAITLLVLRIIQSFSCGAEYLNSSAYLVENAEASKKGYSGSWASFGAMSGMLIASL
                     VVLIVTYFTSHYPEQDWMIWRVPFVLALLGSSIGLYIRLCIPESMEYIVYYADRPKPK
                     FKSLLSESVTYIKNNKIQSLYVFILSCLGVTTTFQIYIYGPMQARLYGNFQNHEIIIS
                     NIISLMVLLGVFPLIGNLSDKISREKIVVAASIGFLVLSQPFFNFLSHKIFYQLILSQ
                     ALIAIPAGAYYATVPVMLAEMFPIKLRCSVLSILYSIAASLSAGLAPLLSMILVKKTG
                     VASSPSILVFTLIATMFIIIIYRTIKPEKRCSLTTLKG"
     gene            complement(3312..4340)
                     /locus_tag="U6309_001963"
     CDS             complement(3312..4340)
                     /locus_tag="U6309_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEN5505402.1"
                     /translation="MRLRLVILVLAAIIIGVGSYSGIVNVIKEPIENVIIGYKSPSAW
                     NNRGVDYVIGKRVAQNDSEAVKWFCKAAKQGEVMAQRNLGLMYAAGKGVPQDNGKAMQ
                     WFRKAALQNDAVSQLNLGVMYQKGMGTQQNDREAIKWIHKAAAQGFPEAERSLGILYS
                     IAENGQQNYVEAFKWLHKAAEKEDAIAQYNLAVMYVTGKGVRQNDTEAVKWFRKAGKH
                     GDLMAQRTLGLMYATGSNVQQDDFQAMKWFRLAAKQGDAVAQYNIGMGFLNGKGVIRN
                     HTKALKWFHLAASQGLPQAQYVLAALYHDGVSLPQNSMEAIKWLRKAAAQGHLQAQER
                     LQALVRQD"
     gene            4525..4956
                     /locus_tag="U6309_001964"
     CDS             4525..4956
                     /locus_tag="U6309_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505403.1"
                     /translation="MNDITSFKIKKIVLLKIIFAVIFSCTTLNACAGTQSYHIKYHKN
                     SKINNISLSNTEVLIEFHRNGNSLGRLLTKTNETQDITIDNTYGKNLVIHVLFIEKEG
                     KRIRCWGVSATKTNTIEIQCDKVVSLKKVEGNTEKATDSTH"
     gene            5052..5261
                     /locus_tag="U6309_001965"
     CDS             5052..5261
                     /locus_tag="U6309_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN5505404.1"
                     /translation="MTNKIRGKVKWFNKDKGFGFIESSGKDYFVHFSSIQSDGFKTLP
                     DGATVLFKMGKGQKGPQAEEVEVIK"
     gene            5341..6084
                     /gene="phbB"
                     /locus_tag="U6309_001966"
     CDS             5341..6084
                     /gene="phbB"
                     /locus_tag="U6309_001966"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEN5505405.1"
                     /translation="MNQRVALVTGGTGGIGTAICQEMQKLDYQVIACYFKQGNHDLAK
                     EWQKKQAMQGFDIDIVYADISSFNDCEKLTDLVIEKYGKIDILVNNAGVTHDATLKKM
                     TQEQWQDVINANLNSVFNMTKTVLSNMLDNSYGRIITISSVNGRKGQFGQCNYAATKS
                     ALYGFTKSLAQEVAKKGITVNTVSPGYINTEMLASLRDDILEAIVAQIPVGRLGKPQE
                     IARVVAFLAEEQSGFITGANFDINGGQYM"
     gene            complement(6235..8028)
                     /locus_tag="U6309_001967"
     CDS             complement(6235..8028)
                     /locus_tag="U6309_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid synthase"
                     /protein_id="HEN5505406.1"
                     /translation="MKGLEKQCCPKKRILTQSQELQEGVDFSCCAPADRGTSDNFFPF
                     FTRLVQANLAKWTAGISPAAIGSSYSTWLWQLAQSPGVLWELAFYPVFHAKDCINNIV
                     CVERAADGKDVRFKKDSWQPMPWRLFAEGFLQMEDWWRRATTDVPGLPNQVERTVSFW
                     ARQCLDALSPSNFVWSNPDLFHEAMRTGGLNLIQGGQIALEDWLEKLTGAPPTGSEHF
                     IPGKQVAITPGRVVFQNHLIELIQYEAQTKTVYKEPILILPAWIMKYYILDLSSHNSL
                     VKWLVSQGHTVFIISWRNPDKEDQDLGMDDYYRQGAMAAFDAVSTLFPETKINLMGYC
                     LGGTLAMITAAAMGRDKDERLNSLTLLAAQGDFTEAGELMLFVTESQVDFLKSMMREQ
                     GYLDTKQMAGSFQMLRAYDLIWSKMVQDYMHGMRRGMIDLTAWNADATRMPYKMHSEY
                     LEKLFLRNDFAEGRYTVEGKPVAAENIKLPVFAVSTEKDHVAPWQSVYKIHLMTEGDV
                     TFVLTGGGHNAGIISEPGHPGRSYRVHEQKQGEAYLNPESWLAMAERREGSWWREWNE
                     WLVQQNTKKRIASSVMNPSLPEAPGTYVLQK"
     gene            complement(8207..9728)
                     /locus_tag="U6309_001968"
                     /pseudo
     CDS             complement(8207..9728)
                     /locus_tag="U6309_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
     gene            complement(9694..10236)
                     /locus_tag="U6309_001969"
     CDS             complement(9694..10236)
                     /locus_tag="U6309_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HEN5505407.1"
                     /translation="MKGIVFTSLNDMIIEQFGIETWDQLVSSLDLPSGGSYTAGGTYS
                     DTEFQQLIQAIAKRTNQHASVFLEAFGEYMFPILSSKYAIFLKKDMTLKEFLKSIDGT
                     IHVEVEKLYPDETLPTISYEEPTANQLVMVYRSHRRLCHFAMGLIQGAAQHFKKKITI
                     KQTHCMLKKDDHCRLEITFE"
     gene            10587..10850
                     /locus_tag="U6309_001970"
     CDS             10587..10850
                     /locus_tag="U6309_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019350007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN5505408.1"
                     /translation="MKKQTDLIVLGKQIRKIRKEKGFSQEGFANFIEMNRGYYGTVER
                     GEANITILNLLKILKGLEVTPNELFPPSTYVSFKRKITKNSAS"
BASE COUNT         3522 a         2350 c         2057 g         3354 t
ORIGIN      
        1 gataattttt tatgacgttc gattacaagg ataattttat gagtaaggga ccctatcaat
       61 cgtttgctaa ccgcttgatt agcgccctca aagatagagg atatacggcc tctcgctcgc
      121 caaatgggat ctgtattaaa accctggctg agtttactgg tgcctctgaa caaatctgcc
      181 gccgttatat acgaggagat gctttaccag actatgaaaa agtcaaacaa ctggctttcc
      241 atcttcaagt aaacccaggc tggttactat ttggggaaga cgaaaatgca acaacgaaga
      301 agaatgaagt cgatgaaaaa ttgcttcatt acatcttgaa acaaagccat catttatacc
      361 ctatctctca gggaagtaat gacgattacg ccgattttgt gttaggattg atcaaagaag
      421 tcaaagcaat tgacacttcg gaaaataatt tactaaaaat cattgacttg gccattggtt
      481 caatttcttc ttatgaagaa aagagaaaaa agcacagtca tgcagtttaa aaaacttgta
      541 gatatggatg taacagctgt ggaagtaaag cttcacccca aagccaagga atttctgttt
      601 gaacactttg tcacaatacg cagggtgttt tcagatgtac tagggcaagt agaaactgac
      661 tatgcttcta ttgcccttat taaccaggca ggacagatct ttttcatatc ctcgaaccca
      721 gccatagagc aaaatttaat tgagcaaagt ctctggctat ttgacggttg ctatcaacca
      781 gaattcctca gccaggatca accgaaatta tggagcgaac taccacatat aggctgcaca
      841 gaatcaattg taaaatacaa acaaataaaa cctagactta taacaggtat ctccatcccc
      901 actgaatatg attcttataa agctatcttt tcatttggat taaaacggat aaatccatat
      961 atccagaaca aaagctcgat tcattgcgaa aaactgttag ccatgggcaa atttgcgtta
     1021 aggcgaattc aagagtattt aattttccct gacaaacagc cctgcatgac gacaaaaccc
     1081 aaactgacat taatcatcaa caatcaggtg ccccatgaac acactccttg ataaaaacaa
     1141 ccctatttta agacaaaccg ctgatccaat ttctgaatca gaatttggta gcagttggtt
     1201 aaaagagctt attaagacta tgtttggcat aatggctgac aagggggctg taggcgtagc
     1261 tgccccccaa attggcatca gcaaacgagt tattgtattt ggtactgatt acaccaaaag
     1321 gagaaaacca gaatacccaa ttcctgatac agccttaatt aatccttcat taaaaatcct
     1381 atctcaagaa attcaaactg gctacgaggg ttgccttaat tgtggtgaat taatgggtga
     1441 agttccaaga gccatggaaa ttgaatattc agggtttgac atagatggaa atagaattac
     1501 caaaaaagct tccggtttag aagcccgcat tcttcaacat gaaattgatc acctggatgg
     1561 atttttattt ttagaccgag tggaagatca agattcacta accactctat cggcaatgca
     1621 aagtaaagga taatcgcatg aagcaccaaa cccgaatcat tctggcaggt acactgggta
     1681 atttaattga atcctttgac atggctattt gtggcctact ctcagtttac attgccaaat
     1741 acctgattgg tgatgtctcc aagggtttgt ttctcgtctt tcttactttc tttgctggat
     1801 acctagccag acctattggg gctatgataa tgggcttact atccgatatc tatggcagaa
     1861 aaattatcct tgcaggttct atcctaacaa tgggcatatc aacaaccctt atcgggttta
     1921 tcccccctca cagtactata ggtacatttt ctgctattac cttattagtg ttaagaatta
     1981 ttcagagctt ctcctgtggt gcagaatatt taaactcctc tgcttaccta gttgagaatg
     2041 ccgaagcctc aaaaaagggc tactcaggaa gctgggcatc ctttggcgct atgtcaggga
     2101 tgctgatagc gtctttagtt gtattgattg ttacttattt taccagtcac tatcctgaac
     2161 aggactggat gatctggcgt gttccttttg tgcttgcgct tttaggatct tcgattggtt
     2221 tatacatccg tttatgtatc cccgagagta tggaatacat cgtatactat gcagacagac
     2281 caaagcctaa atttaagagt ttactttctg aatcggtaac ctatatcaaa aacaataaaa
     2341 tacaatctct ttatgtgttt attttaagtt gtttgggtgt aacaactaca tttcaaattt
     2401 acatttatgg tcctatgcaa gctcgtttat atggtaattt tcaaaaccat gaaattatca
     2461 tttccaacat catttcttta atggtacttc taggagtatt tcctctgatt gggaatctat
     2521 ctgataaaat tagtagagaa aaaatcgtag ttgcagcaag tataggattt ctagttctat
     2581 ctcaaccttt tttcaacttc ttatcgcaca aaatatttta tcagttaatt ttgagtcaag
     2641 ctttaattgc tattccagca ggagcatatt atgcaacagt accagtaatg ttagctgaaa
     2701 tgttcccgat taaattacgt tgtagtgttt tatcaatact atattccatt gcagcaagtt
     2761 tatccgctgg cctcgctcca ttactttcta tgatacttgt aaaaaaaacc ggcgttgcat
     2821 cgtcaccatc aattttagtt tttacgctaa tagcaacaat gtttataata ataatttaca
     2881 gaaccataaa acctgaaaag agatgctctt taacgacact taaaggctaa aataataaca
     2941 tcgcatggca atgtgcttcg gataaaagta ccgctcagat tacttaatga tagtgttgca
     3001 aaaatattcc caataaatga ggcagattct aacttttagg tgtatcaatt ttaacctaaa
     3061 tgaattttca gaaatttaaa cggcttatat ctagaaccta gatagaaaat aacaactcag
     3121 actgtaaagg tgttaatctc tgaaatgacg agctgtgctt atcccattac aattaaataa
     3181 gcattacaaa atcgtgaacg aaaatattat tatgtttaaa taatttctca ttgaaataat
     3241 caaatcgtta ttttcagcat agggggataa atactaactc gtatgataat gaaagaggaa
     3301 ccgcctcaca gctaatcctg tctaaccaga gcctgcaggc gctcttgagc ttgcaagtga
     3361 ccttgcgcag cagcctttcg gagccacttt attgcttcca tgctattttg aggaagactc
     3421 actccatcat gataaagtgc agcaagcaca tattgagctt ggggcaatcc ttgactagca
     3481 gctaaatgaa accatttcag agcttttgtg tgatttcgaa tcaccccttt gccgttaaga
     3541 aagcccatac ctatattata ttgagccaca gcatctcctt gtttcgcggc cagacgaaac
     3601 catttcatcg cttgaaaatc atcctgttga acattgctcc ccgttgcgta cattaatcca
     3661 agtgttcgtt gagccatgag atcgccatgt ttgcctgctt tacgaaacca cttaactgct
     3721 tccgtatcat tttgccgaac ccccttgcct gtgacataca taactgcaag attatattgg
     3781 gctatggcat cttctttctc agctgccttg tgaagccatt taaaagcttc gacataattt
     3841 tgttgcccat tttcagcaat ggaatatagg atgccaagac tacgttcagc ctctggaaat
     3901 ccttgtgcgg ctgctttatg aatccacttg attgcttccc tatcgttttg ctgagtaccc
     3961 ataccttttt gatacatgac accaagattt aattgtgaca cggcatcatt ttgaagagcg
     4021 gctttacgaa accattgcat agccttgcca ttatcctgag ggaccccttt gcctgcagca
     4081 tacatcaagc caaggttgcg ctgggccatg acttcgcctt gtttcgctgc cttgcaaaac
     4141 cattttacag cttcagagtc attttgtgct actcttttgc caatcacata atctacacca
     4201 cgattattcc aggctgatgg gcttttatac cctattatca cattttctat gggctcttta
     4261 atgacattca ctatcccaga ataacttccc acaccaataa taattgcggc taaaactaag
     4321 atgacaagac gtaaacgcat actaaactaa tccttgttag agtcgttaat aaaaactaaa
     4381 atgagtctcc aacctcataa taagttattc tgttgactga gctctattat gacaaaaagg
     4441 aattatgcta ttaaagattg ggcatgtaaa aattttatga caaaatagta aaaatagatg
     4501 agcaagaata agaataggaa tcccatgaac gatatcacgt catttaaaat aaaaaaaata
     4561 gtccttctta aaattatttt tgcggtaatt ttttcgtgca cgacacttaa tgcctgcgcc
     4621 ggcacgcaat cctatcacat caaataccac aagaactcca aaataaataa tatatcctta
     4681 tccaatactg aagtattaat agagttccac cggaatggca attcactggg aagactcctg
     4741 acaaagacga atgagactca agacatcacc atcgacaaca cttatgggaa aaatttggtt
     4801 attcacgtgc tattcatcga gaaagaggga aaaagaatac gatgttgggg agtatctgct
     4861 actaaaacaa ataccattga gattcaatgt gataaagtgg tttcgttgaa aaaagtagag
     4921 gggaacactg aaaaagccac tgattccact cattaaacca ctaaaagctt tctggttagc
     4981 aagtattgac aaaaagaaca gcgtcgcatg acactatata acccacttga ttataaggaa
     5041 ataaacaaaa aatgactaac aaaattcgtg gtaaagtcaa atggtttaat aaagacaagg
     5101 gttttggttt tattgaaagc agtggcaaag actactttgt ccactttagt tcgattcaaa
     5161 gtgacggctt taaaacactt cccgatgggg caaccgttct ttttaaaatg ggaaaaggac
     5221 agaaaggacc tcaggctgaa gaagtagaag tgattaaata acgccattag gcttatgcta
     5281 ataccatcct aataacaata aaactatttt ttctgcataa aaccatcaag gaggaaggaa
     5341 gtgaaccaac gagttgcttt agtcacaggt ggtactggtg gtataggtac tgccatctgc
     5401 caagaaatgc aaaagttaga ttatcaagtc atcgcctgtt atttcaaaca gggcaatcat
     5461 gacttagcga aagaatggca gaaaaaacaa gccatgcaag gatttgatat cgacattgtc
     5521 tatgccgata tttcttcctt caatgattgt gagaaactga ccgacttggt catcgaaaaa
     5581 tacggcaaaa tcgatattct agtgaacaat gccggtgtga cacacgatgc cactttgaaa
     5641 aaaatgactc aagaacaatg gcaggatgtc atcaatgcca atttgaatag tgtctttaat
     5701 atgacgaaaa ccgtgttatc caatatgttg gataatagtt atggacgtat tatcaccatt
     5761 tcttctgtga atggacgcaa aggtcaattc ggccagtgta attacgcggc cactaaatcc
     5821 gcattatacg gttttaccaa aagtctggca caagaagtcg caaaaaaagg cattacagtg
     5881 aataccgtct ctcctggcta tatcaatact gagatgctcg cctctctaag ggacgatatt
     5941 ttagaagcga ttgttgccca aattcctgtg ggacgattag gcaaacccca agaaattgct
     6001 cgtgttgttg ccttccttgc agaggagcaa agtggtttta ttactggtgc caattttgat
     6061 attaatggtg ggcaatacat gtaactcact caaaagtcca ttgagcactg ttattttaaa
     6121 cattgatagg gtggccgcca tccacctaga ggatttgcta agcaaatgga tgcggcttta
     6181 ttggatcgga cgcttactaa tatacagcgc tcctattgtt caaagaagag cgttttattt
     6241 ctgaagcaca taagttcctg gtgcctctgg cagtgaggga ttcatgaccg atgatgcaat
     6301 gcgtttttta gtattttgct ggactagcca ctcattccat tctcgccacc aggaaccttc
     6361 ccgcctctct gccatcgcaa gccagctctc aggatttaag taagcctcgc cttgtttctg
     6421 ctcatgaaca cgataagagc gtcctgggtg acccggctca ctgatgatgc ccgcattatg
     6481 cccaccacca gtgagaacaa aggtcacatc cccttcagtc ataagatgga ttttatagac
     6541 ggattgccag ggtgctacat ggtctttttc agtgctcacc gcaaaaacag gtaacttaat
     6601 attctcagca gccacaggct ttccttccac tgtataacgc ccttcggcaa aatcattcct
     6661 taagaagagt ttttcaaggt attcactgtg cattttgtaa ggcatccggg tggcatctgc
     6721 gttccaagcg gtcaaatcaa tcatccctcg tcgcattcca tgcatgtaat cttgaaccat
     6781 tttggaccaa attaagtcat aagcacgcaa catctgaaaa gaaccagcca tttgcttggt
     6841 atccagatac ccttgttccc gcatcatact tttaagaaaa tccacttgac tttcagttac
     6901 aaagagcatt aattccccag cctcagtaaa atccccttgg gctgccaaga gtgtcaagct
     6961 gtttaaacgc tcgtccttgt ctctgcccat cgcagctgcc gtaatcatag ccaaggtacc
     7021 tcctaaacaa taccccatca gattgatttt agtttctgga aaaagagtcg ataccgcatc
     7081 aaatgcagcc atagctcctt gccgataata atcatccatc cccaaatcct ggtcctcttt
     7141 gtcaggattt cgccaagaaa tgataaatac ggtgtgtccc tgacttacga gccacttcac
     7201 caaggagtta tgtgatgata aatcgagaat ataatatttc ataatccagg ctggtaagat
     7261 gagtatcggc tctttataga ctgttttcgt ttgggcttca tattgaatga gctcaattaa
     7321 atgattttga aagaccaccc gccctggagt aatagctact tgtttccctg ggataaaatg
     7381 ttcagaacct gtaggtggtg caccggttag cttttctaac caatcttcga gcgcgatttg
     7441 gccgccttgg atgagattaa gtcccccagt gcgcatggct tcatgaaata aatcaggatt
     7501 agaccaaaca aaattggaag gggatagggc atcaaggcat tggcgcgccc agaatgaaac
     7561 agtacgttcc acttggttgg gtaatcctgg cacgtccgtt gtggcgcgtc gccaccaatc
     7621 ttccatctgc aaaaaccctt cggcgaataa acgccaaggc ataggctgcc aactgtcttt
     7681 tttaaaacgc acgtctttac catccgcagc acgctcaaca caaacgatat tattaatgca
     7741 atccttggca tgaaaaaccg gataaaaagc aagttcccac aaaaccccag gggattgggc
     7801 caattgccaa agccaggtgg aataagaaga accgatggca gcaggactta ttcctgccgt
     7861 ccatttagcc aagttggctt gcactaatcg agtgaaaaat ggaaagaagt tatcggaagt
     7921 acctctatca gccggtgcac aacacgagaa atctacccct tcttgcaatt cttgactctg
     7981 agtgagtatt cttttcttag gacagcattg tttttctagt cccttcatgg ccgacatccc
     8041 tgatattatg ctctataatc aacacgatat aatacagcat agcacaaaat ttaacccatc
     8101 gattttggtc aataaagcgg ggcttattaa cttatccatt ttaggctaaa ttatttttgg
     8161 taacctaact ggtgttattt tttgatagga atgaaacaac ataaagctac agccctttgg
     8221 agttcctttc ctcgataaat tgcgtcatct gtcctactgt taacggttta ctgctgtaat
     8281 agccttgcac ttcgaaacag tgacttgacc gtaaaaaatt gagttgttct tctgtttcga
     8341 caccacccac aaccacattc aatcctaact cttgcgacaa agcaatcgtc gatttgataa
     8401 ttaaattatc attttccctg agatgagcat cttcaattaa ggtttgtttc agtttgatgg
     8461 attggactgg tgaatttttt aagtgagata aaaaagaata gcctgagcca aaattatcaa
     8521 aagcaagctt tacccccata ttgcgaagcg tacttaatgt atcttcaatc tttcccaaaa
     8581 aattgactgc cgttgcttcc gtaatttcaa gctctaacca atccgatggc atttgatatt
     8641 cttgtaaaat gtgcttcaca gaagaaataa accctttgtg ttgaaattga tgaagcgaaa
     8701 tattaatcgc cagggtgcag ttgaaaggag cgtaactctt tttccaatcc acatactgcc
     8761 tacacgcggt tcgtaagacc cattcaccaa ttggaataat caagttaaaa tgctctgcaa
     8821 cggaaatgaa ttcatcagga aaaacaacgc cccgcttggg atgttgccaa cgtaataaga
     8881 cctccattcc aaccatagaa ttcgttttta agtcaacccg gggctgataa acaagaaaaa
     8941 attcttgtcg ctccaaagca agatgcaact cggttcctat tcccaagcgt tttgaatatt
     9001 gttttctaag agacggtgtg aagatttggc aattatttcg accacattct ttggcgttgt
     9061 ataaagcaat atcggcattc ttacgaaggg ttattgcctc ctcaccagca tcaggaaagt
     9121 aagcaattcc tatacttagt ccaatcatga cggtatggcc tttaataagg aatggttgac
     9181 ccatcgtttg aatcatatta ttggccacta attttgcctg ttgtggatta ttaatttctg
     9241 tcagaatcac tgcaaactca tctcctccca atcgagccac acaatcttct tcgcgaagac
     9301 ttgagcaaag acgcttcgct acttcacgca atagaccatc accgacatca tgaccaaagc
     9361 gatcattcac atttttaaaa aaaatccaaa tcgatgctta acagagccag atgacgaccg
     9421 tatctttttg acctagcaat ctcacgtttt aaatccagtt caaactgcaa tcgattgggt
     9481 aatcggctta atacatcaaa atgggctaat ttcgataatt tcttttgtac gctttctaag
     9541 tcttttagcg tttttagcag ttcagaattc tttttttcca aatcctggtt cagtaaatga
     9601 agttcccttg tcttattttc caataataat tcggcttcct cgcgagcttt tctttcgcga
     9661 taataagagc gctcaaataa tgctttgttc tcatcactca aaggtaatct ccaaacgaca
     9721 atgatcatct ttttttaaca tgcagtgagt ctgcttaatg gtaattttct ttttaaaatg
     9781 ttgcgctgct ccctggatga gccccattgc aaaatgacag agtcttctat gcgatcgata
     9841 caccataacc aattggtttg cagtgggctc ttcataacta atggtaggta acgtttcatc
     9901 cgggtataac ttttctacct ccacatgaat tgttccatca atacttttta aaaattcttt
     9961 taatgtcatg tcctttttta aaaaaattgc gtacttactt gataagatag gaaacatgta
    10021 ttcaccaaag gcctctaaaa aaacagaagc gtgctgattg gtcctcttcg caatggcctg
    10081 aatcaattgc tgaaattctg tatccgagta agtgccgcct gctgtataac ttccaccact
    10141 tggaaggtct agtgaggata cgagttggtc ccaggtttct atgccaaatt gttctataat
    10201 catgtcattt aaggaggtaa aaacgatacc tttcatctat ggtctccagc ataaaacaaa
    10261 tctttaaatc ccttttaatt aagtagcacg tcatttctca atactaaaat tggtttacct
    10321 acagatgaat tcctcatgag tccgctcaat ttaactaaag aactctaacc tactttaatt
    10381 cctgataact atccagtgag ccaggtatta ttttcttctc ctaatcgtat caatatagtt
    10441 caaataacga ttatttaccg tgttaaaaat taacaatgtt aaaaataaca aaataggatt
    10501 ttatttttac catttcctaa acaaaactca aaaatgatag acataccgtg tggctatata
    10561 tgaagcatag cacatatgct ttttgaatga aaaaacaaac cgacttaatt gtactaggca
    10621 aacaaattcg caaaatcaga aaagagaaag gtttttctca agagggtttt gccaatttca
    10681 tcgaaatgaa tcggggttac tacgggactg tggaacgtgg cgaagccaat ataaccattt
    10741 taaatcttct aaaaattctt aaaggattag aagtcacacc aaatgagttg ttcccgccct
    10801 caacttatgt gagcttcaag aggaaaataa ctaaaaactc agcctcataa accataatga
    10861 gtaaggggct aatctaatgc ttgataaaga agtgggaaat tcttaatgcc gttgaaataa
    10921 aaacccatgt aaaaaatgct tgcaggataa ggaaaattaa taaagttata acaaaagtac
    10981 ttgttttcat atactcaata ttttcatacg atatcaggca ttaattatca ctaataatga
    11041 gtgttcattg ggtcatttct aatggtgtca atgcccatgt ctgtggtgat gatagccatg
    11101 atagccacca aatcgcttct taactggcat gttacctttt gagtcctctt taggaatgta
    11161 ggaactgcag gaaaacaata aaaagaacaa acaaatgaaa attagcccta ttgatttttt
    11221 tactaaagtc gaatgcttca tacttattct cttccctttt tgagcttatt tattggatgt
    11281 taa
//
LOCUS       DASLOE010000098        11102 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.099, whole genome shotgun sequence.
ACCESSION   DASLOE010000098 DASLOE010000000
VERSION     DASLOE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11102
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.099"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            9..815
                     /locus_tag="U6309_001971"
     CDS             9..815
                     /locus_tag="U6309_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505409.1"
                     /translation="MNLAITWAAQEGWNPGIHDATCFYQTDPHGFFAGKLNGKTIAIG
                     SAVIYDEFFAFCGFYIVDKSYRDHGYGLELTKARLAYIGNRNAGIDGVINMLDKYARI
                     GYQLAHNNARYCLNKTIKSISSEPHIVPLNQIDFSQLSSYDRLHFPAPRPQFLKCWIN
                     QPQSLALAYLIDGQIKGYGVIRTCHKGFKIGPLFADTLEIANHLFTNLVPYAKGQLIF
                     LDIPENNPLAIELVRQYQMEKVFATARMYLKGEPLVKKESIYGITTFELG"
     gene            819..1709
                     /locus_tag="U6309_001972"
     CDS             819..1709
                     /locus_tag="U6309_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN5505410.1"
                     /translation="MTRDLTGYGSEQINLTWPNKAKIAINFVINYEEGAELTPLNGDT
                     KAEFYGSDFPFVPKGNAERNYSIESIYEYGSRVGIWRLIRLFDKHSIPLTFFVTGYAL
                     ILNPEFSSYLASHNHEVAGHGWRWIDYSKESKATEKKHISRCIDTLQELTGQRPLGWY
                     TGRRSPFTRDLLTQIGGFVYDSDSYADELPYFINKHLVIPYSLDCNDFRFTISPGFVT
                     AQDFYQRLKNTFDYLYQENRLSIMTIGLHPRLSGKPDRCHALNEFLSYIRQFQNIWIA
                     KRIDIALYWLTKYPQIKQDL"
     gene            1926..3140
                     /locus_tag="U6309_001973"
     CDS             1926..3140
                     /locus_tag="U6309_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5505411.1"
                     /translation="MSHLLDLSILKKNRDFTLLYLGQFISFIGTMITSVALPYQIYHL
                     TESTMMVGLLSLAQLLPLLITALLGGVFADRYSRRGLLIISELLLAVGCLFLAFNSKL
                     VNPSVSLIFIIASLMSAITGLHRPAFDSVIQQIVKPEDYKKVGALGSFKFSFCMIIGP
                     AAAGLIIAQYGIVITYLIDLLTFIISLLNLSLMHTIPKPLAKEHPSIIVSLKEGIGFA
                     LRRQELMGSYFVDFFAMVFAMPNALLPAIAQQFGGAKTLGLLYAAPAIGSLIISFVSG
                     WTAKITHDGKAIAIAAALWGVAIIGFGLTTSLWLSLLFLALAGAFDAISGIFRSSLWN
                     HTIPQELRGRLSGIEMISYLSGPRLGDTRAGFVAAGLGIPAALISGGILCIIGVTVCC
                     YALPKFWNYHSK"
     gene            complement(3153..4469)
                     /locus_tag="U6309_001974"
     CDS             complement(3153..4469)
                     /locus_tag="U6309_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1639 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505412.1"
                     /translation="MAGIYLKYYVPVNQIGENMMINPIEYHPKGDKVNSDYFNEYKTK
                     IYERRLSSGLEDLFGDMCGVVVQVQTGDAIPYIKELYSMTPYRYSRSYISNTHKIYCL
                     LNTKNSPAFLVLEPLDPNFKDDITRLNKMYPNSRAKFNARYVGEIYKCKDKDETRNIL
                     VSHDFNFHNPDMTENKFYCNKHIHFTRLSDFTYNCAGYTDDNIYDFDCLELGQRFYLT
                     KEQEAVLDAKDQESHDNGIKDLIKGIDHMATRILAGEREDAILEFLCLSKYYFWGAYN
                     IYDMNSSTNVNRNPHGHDIKSPAKVFTANNTPFMVNSFENLPMPTETFVRNYGRRMHH
                     IAYEVKDGDHSSGKKNIDFVVETLRDKLNIEFLAKVFGACEDSPDLKQIFSKHSIYSI
                     LITEYVERCHNFDGFFTKENVAALTEAASLDETTKQQHKKASIIGD"
     gene            4560..5117
                     /locus_tag="U6309_001975"
     CDS             4560..5117
                     /locus_tag="U6309_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEN5505413.1"
                     /translation="MEALIVSLGVITLAEIGDKTQLLAFLLAAQFKKPLPIILGILAA
                     TLINHSLAGLIGIWITTLLKPNVLRWILGISFILMAIWTLIPDEIEQDEKKISKYLGV
                     FGATFITFFLSETGDKTQIATIALAAHYSSPILVIAGSTLGMLLADLPAVYFGNLFSH
                     KIPMKIIHAVAACAFLIIGLITILF"
     gene            complement(5135..7105)
                     /locus_tag="U6309_001976"
     CDS             complement(5135..7105)
                     /locus_tag="U6309_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEN5505414.1"
                     /translation="MKGVCLGLIICFFVYHDGWAGKRLPELDDLYRIQDISEPDLSPD
                     GKWVAYTVSQLNAKDNISTTEVWKVSVDSKSSQRITFAEDSSSSLPKWSPNGKWLAYL
                     SDGGKEGTTQIWIASVQDNKEHQLTHIDGEVSDFTWSPDSNRIAFIAQKSDPSNEEEQ
                     PIVVDRYQFKVDGVGYLGKGRDHLYLVELKSKAITQLTDGNHDEYLPAWSPDGKYIAF
                     VTKRGKDPDRHFNYDIYLIEPKSGAKEHQLTHFPGSDMNPDYDSNLSWSPDSSKIAYL
                     RSNEHQWTYYSPNQLAIIDINTKDEKIIAPRDNWYYKPKWSQDGKFIYALIEESRNTY
                     LNRIDIHSGQIKKLTGGLRYDMDYSLAKGKIILLTTDDTHPSELFILNSGLIPLTQHN
                     KKLLDEVKFQPAEDIQFNSFDGTRVDGLLMKPAEYQPGILFPGFVYLHGGPVYQFSHE
                     FNFDMQWIAANNYAVIAPNPRGSSGRGFHYAKAIFADWGNLDVKDILASVDYVIGKGM
                     VDPNRLGIGGWSYGGMLTNYVIATDSRFKAAISGAGAANILAGYGVDQYTPEYELELG
                     KPWTNPELYLKLSYPFLKANHIKTPTLFLCSGLDFNVPCVGSEQLYQALKSLDVPTQL
                     VIYPNEYHTLEKPSFVIDRLKRYTNWLDAYVK"
     gene            complement(7315..8568)
                     /gene="sidB"
                     /locus_tag="U6309_001977"
     CDS             complement(7315..8568)
                     /gene="sidB"
                     /locus_tag="U6309_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SidB"
                     /protein_id="HEN5505415.1"
                     /translation="MAKIYNAPKPKYSGWEWFKFIAIRTVFPPVLLWDLIKIGANKLL
                     GEWVSGLVLPAQNENFDDLAVSDDTVSNYNEDDLICEKHDVITHDGAHLDTFEVRHRS
                     QESIDPKYQKYIINLVGNGMCYEHIIDDIKEDSKVLKANVIGFNLRGVGQSTGKAKSS
                     EDLVADGIAQVQRLLDQGVSPQNITLKGHSLGAGVASLVAQHFHQLGQPINLFNSRSF
                     STITNFLVGHMRLERDEIGRAIGHKDSTVGTILGWLAKPFIKLGVALAKWEINAGSAF
                     KSVPEAYKDYIVVRSRKEIRGERIDDAVIPHYASIHKELASERHKKKAEIDEEIANLD
                     DIIRKADPLAKPGLANARDALVQAREKIKSDRKMETDVQYANGHNSDWNALHNRSGKS
                     AQTFFREFVQRTEADHAVKSIPEIN"
     gene            complement(8819..10087)
                     /gene="thrC"
                     /locus_tag="U6309_001978"
     CDS             complement(8819..10087)
                     /gene="thrC"
                     /locus_tag="U6309_001978"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEN5505416.1"
                     /translation="MKIRIKSTRGQCVSTIDEAILSGLAGDGGLFVPDRFPNLTIKKF
                     YEYGNLIDFSVNLLSPFFSDSKIDVNNAFFQKFLTFSFPLHHLFKKSYVLELFHGPTL
                     SFKDLGTQFFSECLLHLIENKSAKVLVATSGDTGAAIAHALHGKRDLEGIILFPKDKL
                     TFRQQSQITCWGENIRALAVNGSFDQCQQLVKLVFAKREHKGVLTTANSMNIARLLPQ
                     MIFYAFTSIQLALRHNHSVNFIVPGGNLGNVTACYWAKMLGFPIEQILIANNTNSALG
                     EFLLTGAYQAKSVTKTLATAMDVGEPSNLERLFILFNNYSECKKQMNAESVSDEQIKQ
                     AILHCYNKYHYIICPHTATAYHRLSTINQDKPWVIVAPGHPAKFEEILEPLLKKEIPV
                     PEQLKVLIGRKQHYSIIEPNYHSLYHFLVE"
     gene            complement(10258..11022)
                     /locus_tag="U6309_001979"
     CDS             complement(10258..11022)
                     /locus_tag="U6309_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEN5505417.1"
                     /translation="MYLGFPVQSILLFLLFSFTPLFVLAAEKTPLNFFLRPNTYFGFS
                     VIRDTAHYNFKRNETSQELNFTDFDHFDWHGSGLGGEIYLGYEKFFLTHYYLGLEGFF
                     NRSSNEGKIYFFDSNELYSRILKGSFKQKWQSGLAIHPGIYLPSVGIIYGRVGWIISD
                     INLSGSITQSGPFGSFSGRFSNTRKKNGVQLGTGLELKLTQNWRCRVEWDWNRLQDFT
                     FDTQGKDSNNHRYQTTRIAKHPILEQFKLGLNWRFS"
BASE COUNT         3352 a         2180 c         1920 g         3650 t
ORIGIN      
        1 aggatgaagt gaatcttgct ataacctggg ctgcccagga aggatggaat ccaggaattc
       61 atgatgcaac atgcttttat caaactgatc ctcatggttt ttttgcaggt aagctgaatg
      121 gaaaaacgat agccattggt tctgctgtta tctatgatga attttttgct ttttgtggtt
      181 tctatattgt tgataagtcc tatagggatc atggttatgg cttggaatta accaaagcaa
      241 gactagctta tattggtaac agaaacgctg gaattgatgg tgtgatcaac atgctggata
      301 aatatgcccg cattggttat caattagcac ataataacgc cagatattgt cttaataaga
      361 caataaaatc aatatcatct gaacctcata ttgttccatt aaatcaaatc gatttttcgc
      421 agttatcatc ctatgaccgc ttgcattttc cagcacctcg tcctcaattt ttaaaatgct
      481 ggataaatca gccacaaagc ttggctttgg cttatctaat tgatggccaa attaagggtt
      541 atggtgttat cagaacctgt cataaaggat ttaagattgg accactattt gcggacactc
      601 tggaaattgc aaaccacctt ttcactaatt tggtcccata tgctaagggt caattgatat
      661 ttctggatat tccagagaat aaccctttag caattgagct ggtccgtcaa tatcaaatgg
      721 agaaggtatt cgctacagca cgtatgtatt taaaaggaga acccctggtt aaaaaagaaa
      781 gcatttatgg aattactact tttgaattag gataaaccat gacccgagat ctcactggat
      841 atggatcaga acaaattaat ttgacctggc ccaataaggc taaaatcgct attaattttg
      901 ttattaatta cgaagaagga gcagagttaa ctccacttaa cggtgataca aaggctgaat
      961 tttacggaag tgattttcct ttcgttccta aaggaaatgc agaaaggaat tatagtattg
     1021 aatctattta tgaatacgga agtcgagttg ggatttggcg tttaatccgt ctttttgaca
     1081 agcattccat ccccttgact tttttcgtaa cgggttatgc cttaatatta aatcctgaat
     1141 tctcaagcta tcttgcaagt cacaaccatg aagttgctgg acatggatgg cgatggatag
     1201 attattcaaa agaaagtaag gcaacggaaa aaaaacacat ctctcgctgt attgatacct
     1261 tgcaggaact cactggacaa aggccattgg gctggtacac tggaagaaga agtccattta
     1321 cccgcgatct gctaactcaa attggtgggt ttgtttatga ttcagatagt tacgccgatg
     1381 aactccccta tttcattaac aagcatcttg taatcccata ctcattagac tgtaatgatt
     1441 ttcgtttcac aatttctccc ggctttgtca cggcacagga tttctatcaa cgccttaaaa
     1501 atacttttga ttacctttat caagaaaatc gattatctat catgactatt ggtttacatc
     1561 cgcgtttaag cggcaaacct gatcgttgcc atgctcttaa tgaatttcta agctatattc
     1621 gacaatttca aaatatatgg atagcaaaga gaatagatat tgcactgtac tggttaacca
     1681 aataccccca gattaaacag gatttatgaa atacccaatc tcattgttag ggaatgtttt
     1741 taattcagtc gtttagttca tctaagattc tccgttaaaa acattttttg ccttgatcgt
     1801 gaggtttttc gtaatgctct tatagtctga tcttattaat ttctgtactg tttcatcaaa
     1861 agagcttaaa attcatatct caaaatcttt ataattcacc ccactataat ttacagattg
     1921 ccaatatgag tcatttgcta gatttatcca tccttaaaaa aaatcgcgac tttactttgc
     1981 tttatttggg ccaattcatt tcattcatag gcacaatgat taccagtgtc gcccttccct
     2041 atcaaattta tcatttgacc gaatcgacca tgatggtagg attactcagc ttggcacaat
     2101 tactgcctct actgattact gcattgcttg gtggagtgtt cgctgatcga tatagccgac
     2161 gcggattact aatcattagt gaattattac ttgctgttgg ttgtcttttt ctggctttca
     2221 attccaaact ggttaatcca agtgttagct tgatatttat aatagcctca ttaatgtctg
     2281 ccattacagg tttacacagg cctgcttttg atagtgtgat acaacaaatc gtgaaaccag
     2341 aggattacaa gaaagtaggc gctcttggca gcttcaagtt cagcttttgt atgattattg
     2401 gaccagctgc tgctggcctg attattgcgc aatatggtat tgtaatcact tatctcattg
     2461 atttattaac ctttatcatt tcacttctca atttgagctt aatgcacacc atacccaagc
     2521 ctttagccaa agagcatccc tccattatag tttctttgaa agagggtata gggtttgctt
     2581 tgcgccgaca agagctgatg ggtagttatt tcgttgattt ctttgcgatg gtttttgcta
     2641 tgccaaatgc attgttgccc gctattgccc aacaatttgg aggagctaaa acactaggac
     2701 tgctttatgc tgcgccagct atagggtcgc tgattatttc ttttgttagt ggctggaccg
     2761 caaaaattac tcatgatgga aaggcgatcg caattgctgc tgctttgtgg ggagtggcaa
     2821 ttatcggatt tggtctcacc acttctttgt ggttatctct cttgtttctt gctcttgcag
     2881 gagcatttga tgccattagt ggtattttca gaagcagctt atggaatcat accatcccgc
     2941 aagaattgag aggccgcctg tctggaattg aaatgattag ttatttgagt ggcccaagat
     3001 tgggtgatac cagagcaggc tttgttgcag caggtttagg catacctgct gcattaatat
     3061 caggcggtat tttatgtatt ataggagtga ctgtatgctg ctatgccttg cctaaatttt
     3121 ggaattatca ttcaaagtaa ttaaccactt tactaatctc caataataga ggctttttta
     3181 tgttgttgct tggtagtctc atccaaactg gctgcttccg ttaatgcggc tacattttcc
     3241 ttggtaaaaa atccatcaaa attgtgacat ctttcgacat attcagtaat taatatggaa
     3301 taaatggagt gtttggagaa aatttgcttt aaatcaggtg aatcctcgca agccccaaat
     3361 actttagcta aaaattcaat atttaattta tctcgaagag tttcaacgac aaaatcaata
     3421 ttctttttac cagaagagtg atccccgtct tttacttcat aagcaatatg atgcattctt
     3481 cttccgtagt tacgcacaaa ggtttctgtg ggcataggga gattttcaaa ggaattcacc
     3541 atgaacggag tgttgttagc agtaaacact tttgccggcg acttgatgtc atgtccgtgt
     3601 ggatttctat ttacattggt agaggagttc atatcataga tattataagc accccaaaaa
     3661 taatatttgg ataaacataa aaactctaaa atagcatcct cacgctcacc agccaggatt
     3721 cgagtagcca tatggtcaat gcctttgatc aagtccttga tcccattatc gtggctttcc
     3781 tgatctttcg catccagcac cgcctcttgt tctttggtta aataaaatcg ttgtcccaat
     3841 tccagacagt caaaatcata aatattatca tcggtatacc cagcacaatt gtaggtaaaa
     3901 tcagataacc gggtaaaatg tatatgttta ttacaataga acttgttttc agtcatatca
     3961 ggattatgaa aattaaaatc atgagaaact aatatgttac gcgtctcatc cttatccttg
     4021 catttataaa tctcacctac ataacgagca ttgaatttgg ctcttgagtt gggatacatc
     4081 ttgtttaatc ttgtgatatc gtctttaaaa ttaggatcca ggggctcaag caccagaaaa
     4141 gcaggagaat ttttggtatt gagtaaacaa taaattttat gtgtgttact aatataactt
     4201 cgagaatatc gataaggagt cattgaatag agttctttaa tatatggaat agcatctccc
     4261 gtttgcacct gaacaactac cccacacata tcaccaaata gatcctcaag tccggatgat
     4321 aaacgccttt cgtaaatctt ggttttatat tcattaaaat agtctgaatt gactttatcc
     4381 cctttgggat ggtattcaat tggattaatc atcatgttct ccccaatttg attcaccggc
     4441 acataatatt ttaagtatat acctgccaat gaatttttat ttgaattaat aactgtttcc
     4501 tctctttcat gacaaaatag aggtataata attcattttg aatccttaca tactttccaa
     4561 tggaagcttt aatcgtatca cttggggtga ttacccttgc agaaattggc gataaaacac
     4621 aattactcgc cttccttctg gcagcacaat ttaaaaagcc attgccaatt attttgggaa
     4681 tattggctgc tactttgata aatcattcct tggctggact catcgggatc tggattacca
     4741 ctttattaaa gccgaatgtt ttgcgttgga tacttggcat atcgtttatt ctgatggcaa
     4801 tatggacgct gatacccgat gaaattgaac aggatgagaa aaaaatatcc aaatatcttg
     4861 gtgtttttgg agcaacattt atcacttttt ttctgtcaga aacaggagat aaaactcaaa
     4921 ttgcaaccat tgccctggca gcacactata gctccccgat cctggttatt gccggttcaa
     4981 cccttggaat gttacttgct gatttaccag ctgtttattt tggaaattta ttttcccata
     5041 aaattcccat gaaaataatt catgctgtcg ctgcatgcgc ttttttaatt attggactca
     5101 ttaccatctt gttttgaccg actaggcaag attgttattt aacataagca tctagccagt
     5161 tggtatatct ttttaatctg tcgatgacaa agcttggttt ctccaaagta tggtattcat
     5221 tggggtaaat aaccagctgt gtgggcacat cgagtgattt caaagcttgg tataattgtt
     5281 ctgacccaac acaaggaaca ttaaaatcca agccactgca caaaaataat gtaggtgttt
     5341 ttatatgatt cgctttaaga aaagggtagc ttaattttag atacaattca ggattggtcc
     5401 agggctttcc taattccagc tcgtattccg gggtatattg atcgacacca taaccagcta
     5461 aaatattcgc tgctccagca cctgatatag ccgccttgaa acgactatct gtagctatga
     5521 cataattggt tagcattcct ccataactcc atccgcctat ccccaaacgg ttcggatcga
     5581 ccattccctt tcctatgaca taatcaacag aagccaatat gtcttttaca tccaaattac
     5641 cccaatctgc aaaaattgct tttgcataat gaaaccccct tccggaactc cctcttggat
     5701 taggagcaat gaccgcataa ttattggctg ctatccactg catatcaaag ttaaattcat
     5761 ggctaaattg atagactggt cctccatgca gatagacaaa accaggaaat aaaatacccg
     5821 gctggtattc agctggcttc attaataatc catcaactct tgttccatca aagctattga
     5881 actgtatgtc ttctgcaggt tgaaatttaa cttcatccaa tagcttttta ttatgttgtg
     5941 ttaaaggaat taatccggaa ttcaaaataa ataattcaga gggatgtgta tcatctgttg
     6001 ttaacaaaat aattttccct ttggcaagtg aataatccat gtcatagcgt aagccgcctg
     6061 tcaatttttt tatctgtccg gaatgaatgt ctatgcgatt caaataggtg tttctgcttt
     6121 cttctattaa tgcataaata aatttgccat cttgagacca ttttggcttg taataccaat
     6181 tatcccgagg tgcaatgatt ttctcatctt tagtattgat atcaataatt gccaattgat
     6241 tgggcgaata ataggtccat tgatgttcat tactgcgaag ataagctatt tttgaactat
     6301 ctgggctcca tgaaagattt gaatcgtaat caggattcat atcactaccg gggaaatgag
     6361 tgagttggtg ctcttttgcc ccagacttgg gttcgatcaa ataaatatca taattaaaat
     6421 gcctatccgg atcttttcca cgctttgtga caaacgcgat gtattttcca tcaggtgacc
     6481 aggccggtaa atattcatca tgatttccat cagtcagttg agtaatagcc ttactcttaa
     6541 gttcaaccaa atataaatga tctcttccct taccaagata accgacacca tcaaccttaa
     6601 attgatatct atctacaact atgggttgtt cttcctcatt agatggatca ctcttctgag
     6661 ctataaatgc aatcctgtta gaatctgggg accatgtaaa atcgctcact tcgccatcaa
     6721 tatgagttaa ttgatgttct ttattatcct ggacagatgc tatccaaatt tgtgtcgtac
     6781 cctcttttcc gccatcggat aaataagcta accatttacc attaggactc catttgggta
     6841 gcgagcttga tgaatcttct gcaaaggtaa ttcgttgact ggatttacta tctactgata
     6901 ctttccatac ctcagtcgtg gaaatgttat ctttggcatt taattgggat acggtatagg
     6961 caacccattt tccatctggt gacagatctg gctcgctgat atcttgtata cgataaagat
     7021 catcaagctc tggcaaacgc ttcccagccc atccatcatg atatacaaaa aaacaaataa
     7081 ttagacccag gcataccccc ttcatgacaa tcatccttat tatgctgaaa aatcagagat
     7141 ttatagtgcc ttaaaaagta tagaataaat ttgttatata tgatttttcc tgcaatcagc
     7201 aaacaaattt tgagtggaat aagtacagtc tggtgtcatt gatttagaat cgtagctgag
     7261 attcttcaga aataaagaaa tccccgtctg ttcaacgagg atcataaaaa ggttctaatt
     7321 tatttctggt atacttttta cggcatgatc cgcttctgtt ctttgaacaa actctctaaa
     7381 gaaagtctgc gcgctcttac ctgatcgatt atgcaatgca ttccagtctg aattatgacc
     7441 atttgcatat tgtacatcag tctccatttt tctgtcactt ttgatttttt ccctagcttg
     7501 aactaaagcg tctcttgcat tagccaaccc tggtttcgca agaggatcag cttttcgaat
     7561 gatatcatcc agatttgcaa tttcttcatc aatctccgcc tttttcttat gccgctcaga
     7621 agccagttct ttatggatag atgcataatg tggaatcaca gcatcatcga tacgctcacc
     7681 ccttatctct tttcttgacc taaccactat gtaatcttta tatgcttcag gaacactctt
     7741 gaatgcactg cctgcattaa tttcccattt agctaaagca acaccgagtt taatgaaggg
     7801 cttagccaac catccaagta ttgtccctac tgtgctgtct ttatgaccta ttgctcgacc
     7861 aatttcatcg cgctctagac gcatatgccc tactaagaaa ttagtaatag tagagaaaga
     7921 acggctatta aatagattaa ttggctgtcc aagttgatga aaatgttgag ctaccaagga
     7981 agccacgcct gcaccaagag aatgaccttt cagggtaata ttttgtggcg aaacaccttg
     8041 atctaatagt ctttgcactt gtgcaatacc atctgcaacc aaatcctcac ttgatttggc
     8101 tttgcctgta ctttgaccaa caccacgaag attaaaacca ataacattgg ccttaagaac
     8161 cttcgaatct tcttttatat catcaatgat atgttcataa cacataccat tccctaccaa
     8221 attgataata tatttttggt atttgggatc aatactttct tgtgacctat gccttacttc
     8281 aaatgtatct aaatgtgctc catcatgagt aattacatca tgtttctcac aaatcagatc
     8341 gtcttcatta taattactta cagtatcatc actaaccgct aaatcatcaa aattttcatt
     8401 ttgtgctggc agcactaaac cactaaccca ttcaccaagc agtttatttg ccccaatttt
     8461 tattaaatcc cataataaaa cagggggaaa aaccgttcga attgcaataa atttaaacca
     8521 ttcccaacca ctatatttag gttttggtgc attataaatt ttagccatga gttcaatcct
     8581 gtttacataa aattgttcat attatataca gttaatatta atttaacctt aagctgaata
     8641 ttataaactt aaaaaaatgt cctaatctga gaagtaaaaa taagtaaagg atttgtgaac
     8701 gctcaatatt ctatcattat gctgacttag cttatgagtt tgagccaatc aaaggcgctg
     8761 gtgataatta accgatgcta ttatatgcca tcacaacaat ttgcttgtgt aatggccttc
     8821 attcaactaa gaaatgatac aaagaatgat agtttggctc tatgattgaa taatgttgtt
     8881 ttctgcctat aaggaccttc agctgttccg ggacaggaat ttcttttttc aatagtggtt
     8941 ctaatatctc ttcaaatttg gccgggtgcc ctggtgccac aataacccaa ggtttgtcct
     9001 gattgattgt acttagacga tgataagctg tggcggtatg agggcaaatt atataatgat
     9061 atttattgta acaatgtagg atagcttgct taatttgctc atcactaaca gattctgcat
     9121 tcatctgttt tttacattca gaataattat taaataaaat gaataaacgc tccaaattgc
     9181 ttggctcacc tacatccatc gctgttgcca atgttttagt aaccgatttg gcttgataag
     9241 ctcccgttaa caaaaattcg cccagtgcac tattagtatt attagctatt aaaatttgct
     9301 caatcggaaa acccagcatt tttgcccaat aacaagccgt aacattaccc aggtttccac
     9361 ctggaactat aaaattaaca gaatgattat gtcttaatgc cagttgaatg ctggtaaaag
     9421 catagaaaat catctgtggt aataatctgg ctatattcat gctattggct gtagtcaata
     9481 ctcctttatg ttctcttttg gcaaatacca gcttcaccag ctgttgacat tgatcaaagc
     9541 tgccattaac agccaatgca cgaatattct ctccccaaca agtaatttga gattgttgtc
     9601 tgaaagtgag tttatctttg ggaaataaaa tgattccttc cagatctctt ttcccatgca
     9661 aagcatgagc aatcgcagcg ccagtatcac cagatgttgc gaccagaact ttggcgctct
     9721 tgttttctat cagatgcaat aaacattcac tgaaaaattg agttcctaaa tctttgaaag
     9781 acagggttgg gccatgaaac aactccagta cataactttt cttaaaaaga tgatgtaatg
     9841 gaaaggagaa ggtgaggaat ttttgaaaaa aagcattatt tacatcaatt ttactatctg
     9901 aaaaaaatgg cgagagtaaa ttaacagaaa agtcaattaa attaccgtat tcataaaatt
     9961 tttttattgt taaattggga aacctgtcag gtacaaataa cccaccatcc cctgccaggc
    10021 ctgacagtat cgcttcgtct attgtggaga cacattgtcc tctcgtactc tttattctaa
    10081 ttttcatctg cagcctttgc accttctgat gaaatattgg caaccaataa atcactttca
    10141 aagacaacta ttcttcacgg tatattgctc acagcaatat ttaattcaat ctaatttaga
    10201 agataatgga agtttagttt attttttaga aatttattaa gattgacgtg aatggcatca
    10261 ggaaaaacgc caattcaaac ccaatttgaa ttgttctaaa attggatgct tggcgattct
    10321 ggtcgtttga tacctatgat tattagagtc cttaccctgt gtatcaaagg taaaatcttg
    10381 caaccgattc caatcccact ctacacggca acgccaattt tgagttaact tcaattcaag
    10441 gcctgtacct aattggactc cattcttttt tctggtattt gaaaatcttc cgctgaatga
    10501 gccaaaaggt ccactctgag taatacttcc gcttagattg atatcactga taatccagcc
    10561 tactcgtcca tagataattc cgacagaagg caaataaata ccagggtgta tagctagacc
    10621 agactgccat ttttgtttaa atgaaccctt taatattctt gagtaaagtt cgttggaatc
    10681 aaaaaaatag atttttcctt cattggaact gcggttaaaa aagccttcta aaccaagata
    10741 ataatgtgtc aggaaaaatt tttcatagcc taaataaatt tctcctccaa gtcccgatcc
    10801 atgccagtca aaatgatcaa agtcagtaaa attgagctct tggctggttt cattcctttt
    10861 aaaattgtaa tgagctgtgt ctctaataac tgaaaatcca aaatacgtat tgggtcttag
    10921 aaaaaaattt agcggtgttt tctcagccgc taaaacaaat aaaggtgtaa aactgaaaag
    10981 taaaaacaac aagattgatt gaacaggaaa acctaaatac acgaataatt ccttttacta
    11041 agtcaaaaaa tatccattat cttctattgt acgtcactat tttgcatcaa tgacaaaagc
    11101 at
//
LOCUS       DASLOE010000099        11084 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.100, whole genome shotgun sequence.
ACCESSION   DASLOE010000099 DASLOE010000000
VERSION     DASLOE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.100"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(145..1470)
                     /gene="hslU"
                     /locus_tag="U6309_001980"
     CDS             complement(145..1470)
                     /gene="hslU"
                     /locus_tag="U6309_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEN5505418.1"
                     /translation="MVMTPREIVQELDKHIIGQDDAKRAVAIALRNRWRRMKIKDPVL
                     RNEIMPKNILMIGPTGVGKTEIARRLANLAKAPFIKVEATKFTEVGYVGRDVDSIIRD
                     LTDMAIKQEREFAMKKVEHLAEDAAEERILDVLLPPARGTLTPGEKNTTARQVFRKQL
                     REGELNDNEIEIEVAATPVGIEIMAPPGMEEMTSQLQSMFQQVGSYRTKTRKMTVAKA
                     MKILREEEAAKLINEEDIKLKAIESVEQNGIVFIDELDKIAKRSDTVSGGDVSREGVQ
                     RDLLPLVEGTTVSTKYGMVKSDHILFIASGAFHVAKPSDLIAELQGRLPIRVELSALS
                     VEDFVRILTEPSASLTLQYSALMETEGLTLTFDETGIRRIAEVAWQVNERTENIGARR
                     LYTVMERLLEVVSFEATDKAGETVHVDKAYVDKNLGQLIADEDLARYIL"
     gene            complement(1472..2020)
                     /gene="hslV"
                     /locus_tag="U6309_001981"
     CDS             complement(1472..2020)
                     /gene="hslV"
                     /locus_tag="U6309_001981"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEN5505419.1"
                     /translation="MEQFHGTTILSVRRGNQVVIGGDGQVTLGNTVMKGNARKVRRLY
                     KDKVIAGFAGGTADAFTLFERFEAKLEMHQGHLIRAAVELAKDWRTDRILRRLEAVLA
                     VADSKASLIITGNGDVIEPEESLIAIGSGGPFAQAAARALMENTQLSAKEIVQKALTI
                     AGDICIYTNNNLTIEELNDEGK"
     gene            complement(2224..3447)
                     /locus_tag="U6309_001982"
     CDS             complement(2224..3447)
                     /locus_tag="U6309_001982"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEN5505420.1"
                     /translation="MTGRVCVLVMDSFGIGASLDAARYGDVGANTLVHIYEACKRGEC
                     DIDGVRKGSLMLPNLASKGLYHAAMASSGLPFIDLSALAIPSGYYGYAVEQSLGKDTP
                     SGHWEMAGVPVTFEWGYFPDKPYCFPEELISEFIKQCNLPGVLGEKHASGTIIIDELG
                     EEHIRTGKPIVYTSADSVFQIAAHEEAFGLQRLYDICKIARNLVDKYQIGRVIARPFA
                     GKPGSFKRTGNRKDYATPPPEKTLLDFLKEDGREVIAIGKIADIYAHQGVTQEIKADG
                     NMALFDATLSAMKTAPQGSLVFTNFVDFDSSYGHRRDVAGYAHALEQFDARLPELEVL
                     LQPNDMVFIAADHGCDPTFPGSDHTREHIPVLMFGPQVNSKFIGRRDCFADIGQSIAE
                     HLQLSSPLAHGVSFL"
     gene            complement(3444..4727)
                     /locus_tag="U6309_001983"
     CDS             complement(3444..4727)
                     /locus_tag="U6309_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5505421.1"
                     /translation="MQVVDEFKAFDVKASLMPWLVCFAATMFFFYEFIQGNMFASIAA
                     NIMQDFQIQADKMAYLSSIYYLANVIFLFIAGMVLDRFSIKNTILIAMFLCVISTFIL
                     SYSDSFYVALFCRFVTGIGSAFCFLGPVRLASHWFPPQRMALVTGAIVTVAMTGGMLA
                     QYPLTKLVLHVGWRQAVQDVGILGLAMLVLMFFWIKERPQVAIKKEGKPINVLVAARK
                     AYLNAQNLRAALYTSLMNMAIAVFGAMMGTLYLEQRLGISPDQAAMINGMLFFGAIIG
                     SPLMGWISDKVGLRVLPMKGGVLASLLILLGVLYLPVSVTMMAVLFFLLGLFTSAQVI
                     SYALVAESSPPMMTATAVSVVSILTQGGYLIYQNLFSALLTNYGDVRLINGVPVYLLE
                     AYQHAAIILPIGLLIALLLLFGLKETRCQQVGHTV"
     gene            complement(4740..6008)
                     /locus_tag="U6309_001984"
     CDS             complement(4740..6008)
                     /locus_tag="U6309_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5505422.1"
                     /translation="MSHSVAVESRTFVPRGDFMAWVVCLSAGLFFLYEFFQLNIFDVI
                     NQSLREDFHIDATQLSWMSSTYLWADILFLLPAGLILDRFSTRKVILTAMFVCVVGTI
                     GFAVTESFFLASFFHFLSGIGNAFCFLSCVVLVSHWFPPRRQALVIGSLVTMAFIGGM
                     MAHTPFAYLNDLFGWRRALLIDGVVGAFLILWIYMIVQDRPEESPAHKLTNEGQILSS
                     FMKALSNKQNWLAGLYTSLLNLPIMVLCALWGASYLQVAHHLPDIAASNVVSLIFMGS
                     VVGCPLVGWLSDTQGRRKPLMIFGAIATLITTIPLFINVVLTQMSLSILFFALGLFTS
                     TQVISYPLVAESNQPENTGAATGIASVIIMGGGGVAQVLFGWLMTHHAGTNVTAYTVS
                     DFQFAMWMFPVAAIAGLVAVLMTRETYCKR"
     gene            complement(6059..6688)
                     /locus_tag="U6309_001985"
     CDS             complement(6059..6688)
                     /locus_tag="U6309_001985"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN5505423.1"
                     /translation="MAKLYFYYAAMNAGKSTVLLQSSYNYRERGMQTLLFTPAIDTRF
                     QYGTICSRIGLSEQAYAFNNSDNLYVLTQEFQLQTQKYSCVLIDEAQFLTREQVYQLT
                     EITDQMSIPVLAYGLRTDFRGELFPGSQFLLAWADELIELKTICHCGRKAIMNMRIDE
                     NGQAVVEGEQVLIGGNESYVATCRLHYKLGEAGKTFPRNKLFNKDTNTF"
     gene            complement(6820..8301)
                     /locus_tag="U6309_001986"
     CDS             complement(6820..8301)
                     /locus_tag="U6309_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEN5505424.1"
                     /translation="MSFQTDKEIQQKFFSYTLFVLVVLLICRLIANYFFPLFDTTESR
                     YAEIARKMLETGNWVTLQQDYGVPFWAKPPLSIWLSAFFMQLLGVNEFAVRLPGLLLS
                     VGVLWLVWGVAKKQSGSMAALFSLLTLAGSLYFFLDAGTVMTDPALLFCTTLASVSFW
                     HAMVYENKIWSYAFFAGLGLGLLAKGPIAVVLVGMVVFFWVLLRNEWLNLWKRLPWFK
                     GTFLLLLIAMPWYVLAELRTPGFLNYFILGEHFHRFLTPGWSGDKYGIVHHVPKGTIW
                     LYAIAGIFPWNIIAGGWLVKHGKMLPSLCQSEDGWLSYLTLWMITPLLFFTFASNIIY
                     PYVFPSLPAFALLFAEILNRLNLELKHLKWVLISSLLCGVLFLAVAFVLGLKPDVVPN
                     THKPIITAWLNQNPTAGSHLVYWGYKTDFSAEFYSEGQVKSVKNQNDLCKLLSNRLEN
                     YLVINSKETQQIPGELLARLTLIDTFRVHRDTLILFHAPVLTC"
     gene            8591..9940
                     /locus_tag="U6309_001987"
     CDS             8591..9940
                     /locus_tag="U6309_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0634 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505425.1"
                     /translation="METNMTFVLKEFDTLKSHFNDTVKIILQREKKDKIEDLPNPRKE
                     ELQFLTAVLNQLEAKIDELKPRSLASYVHVFYGAMLLVCKDVENNLRVMEKKENSLLF
                     TRLMDGMGISDENIPTSEQNIMFYRGLNKFLNFIYESNDSRKGLKKEHFLQVLSLKKI
                     YSLAKLSYEQEEAAENNALAKLTADGKTKANANSFHVEKPIDSSIVEQFKSWDEMKGA
                     LHQLILDELSDKNVAKISALSQARSAQLKFLQTMAEQLDKIPNQSLEPSEKMAILAGA
                     MYIVRGQIAQEYGKDPLSNDKISATVIHTGLSTILHANADCCEDKEVLIAAANKFIRH
                     MVIERPEQSNKKITKESIRENNMFSDIAGFQLISVLTLIQNMIKTCRTDAIEACVTKR
                     KEELEALKPKKEGYSIASSVTGYVGSWFKKAPSISEEDEEDDLKDQNTAEETSKPTV"
     gene            10116..10976
                     /locus_tag="U6309_001988"
     CDS             10116..10976
                     /locus_tag="U6309_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN5505426.1"
                     /translation="MKIKTVTRYLLLFSIFFLIGFNNVFAQKKAIAITIDDLPFVGEY
                     RNFHLNMMMNTMKDEKIPATGFIIAKEVRPDNWEILHKFRDAGFGLGNHTFSHANLNK
                     LKAKEYIQEIKEADSLLMPVLTEPKYFRYPYLAMSSGNKKNKILCYLEKHHYQVAPIT
                     IDSKDFVFNQRLLSVPELGRRTYLDELKPFYLDFIWQQTLKAEEHTQYHHNPDQAQIL
                     LIHANLLNAYVLPDIINLYKQNGYTFVSLEDALKTFKENYHCHKTRILAKKPVGEETD
                     LNIESFMDWD"
BASE COUNT         3627 a         2443 c         1987 g         3027 t
ORIGIN      
        1 attgccttgc tctttgtaaa agtaaattgc atggttggac actcagaatc acatgaggga
       61 tatctctttt tcgagaaata tcccccacaa aaagttgatc tgttaaaaag atttcattct
      121 tgatttcagt ttaactattc tggattacaa aatatagcga gccaaatcct catcagcgat
      181 tagttggcct aaattcttat cgacataggc tttatcaaca tgcactgttt cgccagcttt
      241 atcagtcgct tcaaatgaaa ccacttccag taagcgttcc atgacggtat ataatcttct
      301 tgctccgata ttttccgtac gctcattcac ttgccaggct acttcagcaa tacgtcgaat
      361 tcctgtttca tcaaaggtaa gagtcaatcc ttcggtttcc atcaaagcgg aatactgtaa
      421 agttaaagag gcgcttggct cagtaaggat tcgtacaaaa tcttccacag agagggctga
      481 caactcgaca cgtatcggca gacgcccttg caattcagca atcaagtcag aaggtttagc
      541 tacatgaaaa gcacctgaag caatgaataa aatatgatcg gattttacca tgccgtattt
      601 tgttgaaacg gtagttcctt caactaatgg caataaatct cgctgaacac cctcacgtga
      661 tacatctccc ccacttacag tatcggagcg tttggcaatt ttatccaact catcaatgaa
      721 cactattcca ttttgctcaa cactttcaat cgcttttaac ttgatatctt cttcgttaat
      781 taattttgcc gcttcttcct ctctcaaaat tttcattgcc ttggcaacgg tcatttttcg
      841 agttttcgtt ctgtagcttc caacttgttg aaacatagac tgcaattggc tagtcatctc
      901 ttccatacct ggaggagcca taatttcaat gccaacaggc gttgctgcta cttcaatttc
      961 aatttcatta tcattaagct caccttcgcg taattgctta cgaaacactt gacgagctgt
     1021 cgtgtttttc tcaccaggag ttaatgttcc tcgagcagga gggagtaaaa catccaggat
     1081 tcgttcttca gccgcatctt ctgccaggtg ctccactttt ttcatggcaa attcacgctc
     1141 ttgcttgatt gccatatctg ttaagtcacg aatgatcgaa tcaacatcac gccccacata
     1201 tcccacttcc gtgaatttgg ttgcttctac tttaataaat ggagctttag ccaaattagc
     1261 caaacgtctt gcaatctccg tcttaccaac accggtaggg cctatcatca aaatattttt
     1321 aggcattatc tcgttgcgca atactggatc tttgattttc attcgacgcc aacgatttcg
     1381 taatgcaatg gctacggcac gttttgcatc atcttgacca ataatgtgct tatctaattc
     1441 ttgaactatc tctcgagggg tcatgaccat attatttgcc ttcatcattt aattcttcta
     1501 tggttaaatt attgttagta taaatacaaa tgtcgccagc gattgttaaa gctttctgga
     1561 caatctcctt ggcagataac tgtgtgtttt ccataagggc tctggctgca gcttgagcaa
     1621 agggaccgcc agagccgatt gcaattaaac tttcttcggg ctcaatgaca tcgccattac
     1681 cggtaataat taaagaagcc ttgctatccg ctaccgcaag cactgcctct aatcgacgaa
     1741 gaattctatc ggttcgccaa tctttagcca attcaacagc tgctctaatc aaatgcccct
     1801 gatgcatttc cagttttgct tcaaaccgtt cgaaaagagt aaaagcatct gctgttccac
     1861 ctgcaaaacc agcaatcact ttatctttat acaatctccg aactttacgt gcattgcctt
     1921 tcatcacggt attacctaga gtaacttgcc catcaccgcc aatgacaacc tgattaccgc
     1981 gtcgtactga aagtattgtt gtcccatgaa attgttccaa aataaaatcc tcaattacat
     2041 tgcagaaatg tctaaatggg gatcattggt aaaaaatcaa tagggattta gagaaaaatt
     2101 aagtgttctt aatatctgaa aatactaatc gtcatgtcca ctcccttacg gtcgtgactc
     2161 ggattgtcgt gactcggatt atcgttcaaa ccgagccacg accgtaaggg agtggatata
     2221 aaattataaa aaagaaacac catgagcgag tggcgaggat aattgcaaat gctccgcaat
     2281 actttgacca atatctgcga aacaatctct ccgtccaata aatttgctat ttacctgagg
     2341 gccaaacatc aatactggaa tatgctcgcg ggtatgatca gatccaggga atgtaggatc
     2401 acatccgtga tctgctgcga taaaaaccat gtcattgggt tgtaagagaa cctccaactc
     2461 tggcagacgc gcatcgaatt gctctagcgc atgcgcataa ccagctacat cacgcctatg
     2521 cccataagaa gaatcaaaat caacaaaatt ggtaaatacc aagctacctt gtggggctgt
     2581 tttcattgcg gataaagtgg catcaaacaa agccatattg ccatcggctt tgatttcttg
     2641 cgtaacccct tgatgagcat agatgtctgc aatcttacct atagcaatca cttcccgacc
     2701 atcttccttt agaaaatcaa gcaatgtttt ttccggagga ggtgtagcgt aatcttttct
     2761 attacctgtt ctcttaaatg agcccggctt acctgcaaaa ggacgagcaa tcactcgacc
     2821 aatttgatat ttatctacca aatttcgagc aattttgcag atatcataga ggcgttgtaa
     2881 accaaatgcc tcttcatgtg cggcaatttg aaatacgcta tcggcagaag tatatacaat
     2941 cggcttaccg gtacgaatat gctcctcacc caactcgtct ataatgatgg ttcccgaagc
     3001 atgcttttct cccaaaactc ctggcaaatt acactgctta atgaattctg aaattaattc
     3061 ttcagggaaa cagtaaggtt tatcaggaaa atatccccac tcgaaagtca caggcacccc
     3121 agccatttcc caatgaccac tgggagtatc tttgcccaag ctttgttcca ctgcatatcc
     3181 ataataccca gaaggaatag ctaaagcaga taaatcgata aagggtaacc cggaactggc
     3241 catagcagca tggtaaagcc ccttgctcgc aagatttgga agcattaaag agcctttgcg
     3301 aaccccgtct atatcgcact cacctcgttt acacgcttca taaatgtgaa ccaaggtgtt
     3361 agcacccaca tctccatacc gagcggcatc aaggctggct ccgatgccaa aggaatccat
     3421 tactaatacg caaactcgtc cggtcatact gtatgcccaa cttgttggca acgagtttct
     3481 tttaatccga acaataaaag taatgcgatc aataatccta tgggtaaaat gattgctgca
     3541 tgctgatacg cttcgagtaa ataaactggc acaccattaa tcaaacgaac atcgccataa
     3601 ttcgtcaata aagcactaaa caggttttga taaataagat acccgccttg agttaatatg
     3661 gacacgacac tgacagccgt tgcagtcatc atgggagggc tgctttcagc aaccagggca
     3721 tagctaatga cttgagctga agtaaataat cctaataaaa agaacaatac ggccatcatt
     3781 gtcactgaaa caggtaaata aagtactccc agcaaaataa gtaaagaggc aaggactccc
     3841 cctttcatag gcagcacacg caaccctacc ttatcagata tccaccccat caaaggtgaa
     3901 cctataatgg ctccaaaaaa caacataccg ttgatcatag ctgcctgatc agggctaatg
     3961 cctaatctct gttcaagata taaagtcccc atcatggcgc caaatacagc aatggccatg
     4021 ttcataagac tagtgtataa ggctgctcgc aaattttgag catttaagta ggcttttctt
     4081 gcagccacaa gcacattgat aggttttcct tcttttttga ttgcgacttg aggtctttct
     4141 ttaatccaaa aaaacatgag aactaacatg gccaagccca atatccctac gtcttgaact
     4201 gcttggcgcc atccaacatg tagaaccagt ttagttaagg ggtattgcgc cagcattcct
     4261 cctgtcattg caacagttac aatcgcgcca gtcactaaag ccatacgctg gggaggaaac
     4321 caatgagacg ccaaacgcac gggacccaaa aagcaaaatg cgctacctat tccagtcaca
     4381 aacctgcaaa ataaagccac ataaaacgaa tctgagtagg agaggataaa cgtactaatc
     4441 acacacagaa acatagctat taaaatggtg tttttgattg aaaacctgtc caaaaccatt
     4501 ccggcaataa acagaaaaat cacatttgcc aagtaataga tgctggataa gtaagccatt
     4561 ttatctgctt gaatctgaaa atcctgcatg atatttgccg caatggaagc aaacatattt
     4621 ccctgaataa attcataaaa gaaaaacatg gttgctgcaa aacaaaccag ccatggcatt
     4681 agcgatgctt taacatcgaa tgctttaaat tcatccacta cctgcattat ttctcctctc
     4741 tatcgcttgc agtatgtttc acgagtcatg agcacagcaa ccagtcctgc aattgcggca
     4801 acaggaaaca tccacatggc aaattgaaaa tcgctaaccg tatatgccgt aacatttgtt
     4861 cccgcgtgat gtgtcataag ccaaccaaac agaacttgag caacaccgcc cccgcccata
     4921 ataatgacag aagcaattcc agttgcagca ccagtatttt ctggctgatt gctttcagca
     4981 accagggggt aagaaatcac ttgcgtgctg gtaaataatc ccaaagcaaa aaagaggata
     5041 ctgagactca tttgagtcag aaccacattg ataaatagag gaatagtagt aatcaaagta
     5101 gctatcgccc cgaaaatcat taagggtttg cgacgtccct gagtatcgga taaccaacca
     5161 accaatgggc aaccaacaac actccccatg aaaataaggc tgacgacatt gcttgccgca
     5221 atatcaggca aatgatgagc cacctgcagg taacttgcac cccatagggc acacaaaacc
     5281 ataatgggta aattaagcag agaggtatac aagcctgcca gccagttttg tttattggat
     5341 agagctttca tgaaactcga tagtatttgt ccttcattgg ttaatttatg tgctggtgac
     5401 tcttcaggcc tgtcttgcac aatcatgtaa atccataata ttaaaaaagc tccaacaacc
     5461 ccatcaatta acaatgcacg tcgccagcca aacagatcat tcaaataagc aaaaggggtg
     5521 tgcgccatca taccaccaat aaatgccatg gtaactaatg agccaatgac taatgcctgt
     5581 cttctgggag gaaaccaatg agagaccaaa acaacacaag ataaaaagca aaacgcatta
     5641 cctattcctg agagaaaatg gaaaaatgag gcgagaaaaa atgattccgt aactgcaaaa
     5701 cctattgttc caacaacaca aacaaacatt gcggtgagaa taactttcct ggttgaaaac
     5761 ctgtcgagaa taaggccagc tggtaaaaga aaaagaatat cagcccataa ataagtgctc
     5821 gacatccaac ttagttgggt ggcatctata tgaaaatctt ctcgaagcga ctgattaata
     5881 acatcaaaaa tattgagttg aaaaaactca tataaaaaga aaagccccgc agaaagacac
     5941 actacccaag ccataaagtc accacgggga acaaaagttc tcgactccac tgcgacagaa
     6001 tgcgacacca cctctccttt atttaaggcc taaaaactgc ggcaaattat atcaaaattt
     6061 aaaaagtatt ggtatcttta ttgaacaatt tatttcttgg aaacgtcttc cctgcttcac
     6121 ctaatttata atgcaaccga caggtagcaa cataggattc attgccgcct atcaatacct
     6181 gctccccctc aacaacagct tgcccatttt catcaattct catattcata attgctttac
     6241 ggccacaatg acaaattgtc tttaattcga ttaactcatc tgcccaagcc agtaaaaatt
     6301 gacttcccgg aaataactcg cctctaaagt cagtgcgtaa cccataagct agtactggaa
     6361 tagacatttg atcggttatt tccgttaatt gatacacttg ctctcgagtt aaaaattgag
     6421 cctcgtcaat caggacacaa gagtatttct gagtttgtag ttgaaattct tgagttaaaa
     6481 catacaggtt atccgaattg ttaaaagcat aagcctgttc ggataaaccg atacgtgaac
     6541 agatagttcc atattggaaa cgtgtatcga tggctggggt aaacaacaga gtttgcatac
     6601 cgcgttctct gtaattataa ctggactgca gtaacaccgt acttttacct gcattcatag
     6661 ccgcatagta gaaatataat tttgccatag atagattatt tccatcctct ttgtttttac
     6721 tcaggactta cgaaaaaccc caaggtcaag gcaaaaaaga ggttggagtt tttcgtaagt
     6781 cctattactt tactacgaga tgaaattgga aaccactgct caacaagtca gcactggggc
     6841 atggaacaat attaaagtat cccgatgaac tctgaacgtg tcgatgagtg tgagtcttgc
     6901 gagtaattcc ccgggaatct gctgagtttc tttactattg ataaccaaat agttttccaa
     6961 tcggttagag agaagtttgc ataaatcgtt ctgattttta accgatttca cttgaccttc
     7021 cgagtaaaac tcagcagaga aatccgtctt gtatccccaa taaacaaggt gactgcctgc
     7081 tgttgggttc tgattcagcc atgcagtaat aatcggctta tgcgtattag gcacaacgtc
     7141 tggttttaaa ccaagtacaa aagccactgc caaaaataaa actccacaga gtaatgagga
     7201 gataaggacc cacttcaaat gtttcaattc caaattcaag cgattcaaaa tttcagcaaa
     7261 aagcaaagca aatgcgggta gagaaggaaa cacatagggg tatatgatat tgctggcaaa
     7321 agtgaaaaaa agaagcggag taatcatcca aagagttaaa tagcttaacc atccatcttc
     7381 actttggcac agagagggca acatttttcc atgtttcacc agccagccgc ctgctataat
     7441 attccaagga aagatacctg ctatagcata taaccatatc gttcctttag gcacatggtg
     7501 cactatcccg tatttatcac cagaccagcc aggggttaaa aatcgatgga aatgctctcc
     7561 caggataaaa taatttaaaa atcctggggt tcgtaattca gccagaacat accagggcat
     7621 agcaattagc aaaagaagaa atgtgccttt aaaccacggt aatctcttcc atagattaag
     7681 ccattcattg cgtagcagaa cccagaaaaa tacaaccatt ccgaccagta ccacagcaat
     7741 tgggcctttt gctaataaac ccaggcccaa tcctgcgaaa aatgcataag accatatttt
     7801 attttcataa accatggcat gccaaaacga gacacttgca agcgtggtac aaaaaagtag
     7861 ggcaggatca gtcatcacgg tccctgcatc aaggaaaaaa taaagcgatc ctgccagtgt
     7921 taatagggaa aagagcgcag ccattgagcc actctgtttt tttgccactc cccaaaccaa
     7981 ccataaaaca ccaacagaga gcaaaagacc tggcaggcgg actgcgaatt cattaacacc
     8041 caatagttgc ataaaaaaag cagacaacca gattgataaa ggtggtttag cccaaaatgg
     8101 aacaccataa tcctgctgca aagtcaccca gttccctgtt tccagcattt tgcgtgcaat
     8161 ttcagcgtag cgggattcgg tcgtatcaaa caaaggaaaa aaataattgg caatcaagcg
     8221 gcatatcagc agaacaacca acacaaacaa ggtgtaacta aagaattttt gctgaatctc
     8281 tttatctgtt tgaaaactca aaatatacct cttttattta aacaactaaa tcggagtacc
     8341 atttagtgaa gcattggagt gagcctgtgg tatctcggac atagcgttcc caggaataaa
     8401 ttccataaag ccaattattc cctaacctcc cttttccttg tgatagatct tcacccccaa
     8461 gccaggtttc ttaattttta aatgattatt atatattttt aaattgtata atgaaaatac
     8521 atgacgtcag cttaagttta gtttacactg gccactgtaa gtatttctta cattaatctt
     8581 acttggattt ttggagacta acatgacgtt tgttttaaag gaatttgata cactaaaaag
     8641 tcattttaac gacaccgtta aaatcatcct tcaacgcgaa aaaaaggaca aaattgaaga
     8701 ccttcccaac cccagaaaag aagaacttca atttctgacc gctgttctca atcaattgga
     8761 agcaaaaatc gatgaactga aaccacgttc attggccagc tatgtccatg tattttatgg
     8821 tgctatgctg cttgtctgta aagacgttga aaataatctg cgggtgatgg aaaagaaaga
     8881 aaacagctta ctgtttactc gcttaatgga tggtatgggt atttctgatg aaaatatacc
     8941 aacatccgag cagaacatca tgttttacag aggattaaac aaattcttaa atttcattta
     9001 tgaaagcaat gattctcgta aaggcttaaa aaaggagcat ttcctgcaag tcctttcgtt
     9061 aaaaaagata tactctttag ccaaattaag ttatgagcag gaagaggcag ctgaaaataa
     9121 tgctttggca aaattaactg ctgatggaaa aaccaaagcc aatgcgaaca gcttccatgt
     9181 ggaaaaacca atcgattcat ccattgttga gcaattcaaa tcctgggatg aaatgaaagg
     9241 cgctcttcat caattaattc tcgatgaact ttctgataag aacgttgcta aaatttcagc
     9301 tttaagccaa gcccgctcag cacaactgaa atttttacaa acgatggcag aacagctaga
     9361 caagatccct aaccaatctc ttgagccgtc tgagaaaatg gccattcttg ctggagcaat
     9421 gtatatcgtt cgtggccaaa tagcccagga gtatggaaaa gatccattaa gcaacgataa
     9481 aatcagcgct actgtgattc atactggttt aagcacgata cttcatgcca atgctgattg
     9541 ctgtgaagac aaggaagtac tgattgctgc tgcgaataaa tttattcgtc atatggttat
     9601 tgaacgccct gaacaatcaa ataaaaaaat cactaaggaa tccattcgag aaaacaacat
     9661 gttttccgac atcgccggct tccaattgat ctctgtcttg acgttaatac aaaacatgat
     9721 caaaacatgt cgtactgatg ccattgaagc ttgtgtcacc aagcgtaagg aagaactcga
     9781 agcattaaaa cccaaaaaag agggttattc cattgcgagt tcagtcactg gatatgtagg
     9841 cagctggttt aaaaaagcac caagcatatc tgaagaagac gaagaggatg acttaaaaga
     9901 tcaaaacaca gcagaagaga ccagtaaacc gaccgtataa taatttgaac aattaacgtc
     9961 gattttcatt cataaacagc cttattttca aaataaggct gtttatttcg tgtttcttat
    10021 tttaataagc tcttaaatat aagctattca tcgtaatttt tgttacaata gtgacaatat
    10081 ttttctcaat tgaccttaca catattaaat ttcgaatgaa aatcaagact gttacgagat
    10141 acttactact tttctcaata tttttcctga taggatttaa caacgtattt gctcaaaaaa
    10201 aagccatagc aatcactatt gatgatttgc cctttgttgg tgaataccga aactttcatc
    10261 tgaatatgat gatgaataca atgaaagacg aaaaaatccc ggcaacaggt tttattattg
    10321 ccaaagaagt caggcctgat aattgggaaa tacttcataa atttcgtgac gcaggtttcg
    10381 gcttgggcaa ccatactttt tctcatgcca atttaaacaa actcaaagcg aaagaatata
    10441 tacaggaaat taaagaagca gattcacttt taatgcccgt cctaactgaa cccaagtatt
    10501 ttcgttatcc ctatttggca atgagttctg gaaataaaaa aaataaaatc ctctgctacc
    10561 ttgaaaaaca tcactatcaa gttgccccga ttaccattga cagcaaagat tttgtcttta
    10621 atcaacgctt attatctgtg cctgaactgg gaaggagaac ttacctggat gagttgaagc
    10681 cattttatct ggattttatc tggcaacaaa cgttaaaagc tgaagaacat acgcaatacc
    10741 accataatcc agatcaagca caaatcctgt taatccatgc taacttactt aatgcctatg
    10801 tcctgcctga tattattaat ttatacaaac aaaatggtta cacgtttgtg agtctggaag
    10861 atgccttaaa aacattcaaa gaaaactatc attgtcacaa aaccagaatc cttgcaaaaa
    10921 agcctgtcgg agaggaaaca gatttaaata tagaatcatt tatggattgg gattaataag
    10981 ctccatgaag tcttcagact gtaaaatttt ttttgccaaa ccaatgtttc tggcccctat
    11041 ataattctct ctatataacc ttgctaaccg gactatcctt aaaa
//
LOCUS       DASLOE010000100        10921 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.101, whole genome shotgun sequence.
ACCESSION   DASLOE010000100 DASLOE010000000
VERSION     DASLOE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10921
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.101"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            163..759
                     /locus_tag="U6309_001989"
     CDS             163..759
                     /locus_tag="U6309_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN5505427.1"
                     /translation="MLEQSLRHYYQLVLVDPILPALRDRITPLSITWLSGIFGLLFIP
                     FILLDKPYIAISFLLLSGYLDTLDGSLARFQNRASDFGSAMDIVMDRIVEFSAVLALY
                     LFDPQHRATLAILMLGSILLCITSFLVVGIFSNNTTQKSFYYSPGIMERAEAFLFFIF
                     MTLFSSHFNFLAALFCFLVCLTALIRLFEFKKSVQAPG"
     gene            867..1457
                     /gene="msrA"
                     /locus_tag="U6309_001990"
     CDS             867..1457
                     /gene="msrA"
                     /locus_tag="U6309_001990"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN5505428.1"
                     /translation="MLRILLYFLLLINTTFCLAKTEKAIFAGGCFWCMEADFDHLPGV
                     LSTTSGFDGDTLKNPTYEQVSAGNTHYAESVMVEFDPNKLSYKKLVEYFFMHIDPTVK
                     NAQFCDQGRQYRSAIFYMNDQQKKIAMEYKKELEQKFPTIYTEIVPSTSFYPAEEYHQ
                     NYYKKNPIRYKYYRYRCGRDARVQEVWGNKKQENNS"
     gene            1562..2422
                     /gene="vipF"
                     /locus_tag="U6309_001991"
     CDS             1562..2422
                     /gene="vipF"
                     /locus_tag="U6309_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector N-acetyltransferase VipF"
                     /protein_id="HEN5505429.1"
                     /translation="MITQVNQLDELQLKDLKALRAECKKNDGSIPNLYIHILKQHRSL
                     PTSFLYYHNGTLIGFLSVYFFYDDAVEVAVLVSPQYRRQGIAKQLIKEALILIKSQNY
                     FNLIFSCPSRLNDNWLTQKGFTYLHSEYFMERDDLNPILDYMRPLSFRMATLEDIPIL
                     CGLDEVCFPDKNQDLVNRFQQILNEREYEIVIAMLNNHPIGKAHIRWQTKRATLSDIA
                     ILPKEQGKGFGSALIAHCINMILSEGKSRVDLDVETHNKKALNLYIQLGFHIQNACDY
                     WSINVNQLAK"
     gene            2547..3776
                     /locus_tag="U6309_001992"
     CDS             2547..3776
                     /locus_tag="U6309_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEN5505430.1"
                     /translation="MKKRVVITGMEITSSIGSGIDKFWLAASQGQCGIKRIQSYDPSP
                     YSTQIAGEITDLSLDHLPEFNKSKRYPRAAQYALFCAHHAIEKAGLEQAELNAAGTFI
                     GTSIGGQPELEAGYKLFFTDSWKKIPPLSVIRGMPNSIANHIAIAFGIGGPNSTISNA
                     CVSSGEAIGMAYQQIAHGNLTTALCGGTESLVWESVMAAWCKLRVMSTNNENPSQASR
                     PFDLNRDGMVMADGAGILILEELQQAKARGAQILGEIIGYGASCDAFHVTAPNSQGQA
                     RAIVAALNDAKLSINDVHYINAHGTGTQLNDSTETETIKMVFGKRAYDIPITAQKAMT
                     GHAIGAAGAMEIIATTLSLQKNTLLPTINLHTPDPACDLDYVPNAAREKTIDIALSNH
                     FAFGGANAALVLRKYIS"
     gene            complement(3773..4555)
                     /locus_tag="U6309_001993"
     CDS             complement(3773..4555)
                     /locus_tag="U6309_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEN5505431.1"
                     /translation="MLKKILLTGLCVLFSMNLSASEPIYDEEIPLLWSSIITVSGGPS
                     WATPGQNQYIYPMSPIYINNFNPNLPPNYYLYFTHDSPTTILANGEIFFGLQRAINPQ
                     MIGQLGLGVAGVSDAKITGDVNLNGLLNVYTYDYKVNHARVELKGKLIGSAFTPVQPY
                     LSGSFGIAFNNAHDYRTLSINQFLFPAPWFASNTTYAFAYSVGAGVQKMLNRNWQVGV
                     GYEFADLGKSYLGGDGVNITKGPRLTHLYNNQLLFSVSYLYS"
     gene            4819..5874
                     /gene="lpxD"
                     /locus_tag="U6309_001994"
     CDS             4819..5874
                     /gene="lpxD"
                     /locus_tag="U6309_001994"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEN5505432.1"
                     /translation="MNSSLRDIANLVQGMVIGNDTITVSTLSPIDNILPGSLVFAEGE
                     DNIKLAENSEAAAILIGQGTIDSPKPLIQVKNPFKAFIALLNHFYPPKRISPGVHPTA
                     VIGAEVQLGDEVYVGPFVVIESGSIIGNRSVLKSHIHIGHNVVIGDHTTIHPQVTIYD
                     NCRIGSNVTIHASTVIGSDGFGYTFVDGQHLKVPHSGYVVIENNVEIGANTAIDKATL
                     GATVIGEGTKIDNLVQIAHSVKLGKHNIICAFTGIAGSTTTGNNVIFAANVGVSDHVH
                     IEDEVILGARTGVPPHKHLKKGTVYLGNPAKPKDVAIKHELSVNRIPLIRKNIKSLTE
                     QVAVINKKLDIKAKEVE"
     gene            5877..8567
                     /gene="polA"
                     /locus_tag="U6309_001995"
     CDS             5877..8567
                     /gene="polA"
                     /locus_tag="U6309_001995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEN5505433.1"
                     /translation="MKPPLILIDGSSYFFRAFHALPPLTNSKGQPTGAIYGVANMIKK
                     IIKDYQPEEIAVVFDAKGKTFRDEWYPEYKAHRPPMPQELSSQFQPLIQLLQAMGLPL
                     LIIDGVEADDVIGTLAKQATEQGIPVVISTGDKDMAQLVNEHVSLINTMSNYSMDIDG
                     VKAKFGVTPEQIIDYLTLIGDSVDNIPGVEKCGPKTAVKWLTEYQTLDNLLAHANEIG
                     GKIGEYLRASIPHLPLSKKLVTIKTDLDLPLNLNQLMPKPADSEQLIKLTRELEFKSW
                     LKELLQEEENQKSPKEMDASNNKSYEIITTHQQLNHWLNKLEQSQQFCIDTETTNLDV
                     MQAELVGISLAVEEENASYIPLAHTDGSTQLVKEEVLTALKPILENPAIGKIGQNIKY
                     DYSVLKNYGITLKGIRYDTMLESYVLNSGAGRHDMDSLALKYLGYKTISYEDVAGKGA
                     KQLRFDQIPVEKAGVYAAEDADITLRLHHKLYPMLDEPLRNVLHDIEMPLLTVLADME
                     MHGVLIDPVILEKHGSRLKEQMKSLEQEAVQLAGKAFNLNSPKQLQEILFDEQKLPVV
                     AKTPTGQPSTAESVLQELAFDYRLPAVILEYRSLSKLVSTYIDALPKRINPKTHRVHT
                     SYNQAVAATGRLSSSDPNLQNIPIRSEEGRLIRTAFIAPEGSLIMAADYSQIELRIMA
                     HLSQDDNLLRAFANGWDIHAATASEIFQTNLEAVSKEQRRRAKAVNFGLIYGMSAFGL
                     AKQIGVERQDAQHYIDTYFRRYPKVLEYMERTRKQAHQLGYVETLFGRRLYLPEINSR
                     NLMRQKAAERTAINGPMQGTAADIIKKTMLAISSWEHSQKNPSAKMIMQVHDELVFEI
                     RKEAVEECHAIIHKLMEQTVKLSVPLVVSIGTGSNWDDAH"
     gene            complement(8597..9049)
                     /locus_tag="U6309_001996"
     CDS             complement(8597..9049)
                     /locus_tag="U6309_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN5505434.1"
                     /translation="MRVLIVEDSAFNAFCLRRLLESVVASISVTIVNNSQAALSFVNN
                     YCPDIVVVDGDLGASPDEMNCNGPELAGILLQKYPQLPIVAWSDSDAMREDFIKVFKS
                     HGLFLNEYNTWPKVIGLERIHKTWSYYFGEFIGGQNPSFASQTSINYY"
     gene            complement(9164..9505)
                     /locus_tag="U6309_001997"
     CDS             complement(9164..9505)
                     /locus_tag="U6309_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEN5505435.1"
                     /translation="MNQLPNCPKCLSEYCYEDGDVLICPECTHEWPKNSNNNDEESAK
                     IIKDANGNLLQNGDTITVIKDLKIKGSSQVIKVGTKVKNIRLVDGDHDIDCRIDGIGA
                     MKLKSQFVKKI"
     gene            complement(9667..10860)
                     /gene="ceg4"
                     /locus_tag="U6309_001998"
     CDS             complement(9667..10860)
                     /gene="ceg4"
                     /locus_tag="U6309_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector phosphotyrosine
                     phosphatase Ceg4"
                     /protein_id="HEN5505436.1"
                     /translation="MPKVIIFTDFDGTVTGRSGNETVFTEFYQSLLQGYKKDVEQDYK
                     NTPMKDPIEAQALFEAKYGKYNENFDHDQQDVDFLMSPEAVAFFHEVLKNDAVTVNIV
                     TKNRAEYIKAVFKYQGFSDEEIEKLTILESGYKFNDVNSQLNHPTERANRVYILDDSP
                     TDYAEMLRAVKGRGYNEEEIRGYCKNPGEFEWSRYLEDVREMFPPKENSPEEVRLPYE
                     QGDEVSIGEETPALLQEDNLSRREETATVLSNQPVPSSDFRTLKIMGAFAGIGFGFGF
                     ALGVTLVATGVFAPSGVGLLGALTLGAISGTGAAALSSAIGFAVAKGTDITPEPVSEE
                     PVPAVKQSSHEAMRGLGGSQLLSTNNIHPVGHFPSVLSRRTPTKGKEEQCQEEYVLTN
                     PLNNS"
BASE COUNT         3470 a         2228 c         2041 g         3182 t
ORIGIN      
        1 taatatttcg tgcccaagct tcgagacgat gcttaggcat ctcctcagcc caaacgggca
       61 tcggatatcg aatgcttctt atagaatact tgtccataac tcacgttaga tagccaaata
      121 actgccataa gctcatagtt ggcctaagcg cgatgaatta ttatgcttga acaatcatta
      181 cgacattatt atcagcttgt cttggtagat cccatactac ctgctttacg tgacaggata
      241 acgccattgt ccattacctg gttgtcggga atatttggct tgttatttat tccttttatc
      301 ttattagaca aaccttatat tgcaattagc tttctcttgc tttctggata tctggatact
      361 cttgatggtt cgctggcccg ctttcaaaac agagcctctg attttggctc agcaatggat
      421 atcgtcatgg atagaattgt agaattttct gctgttcttg ctttgtatct ttttgatcca
      481 caacaccgtg ccactttagc cattttaatg ttaggcagta ttttgctctg tattacaagc
      541 tttctggttg tgggaatttt ttccaacaat actactcaaa aaagttttta ttacagcccg
      601 gggataatgg agcgtgccga agcatttcta ttctttattt ttatgacatt attttccagc
      661 cactttaatt ttcttgccgc tctcttttgt tttttagtct gtttaaccgc gttgataagg
      721 ctttttgaat ttaaaaaaag cgtacaggct ccaggttagc gaaacaataa attttccgaa
      781 gcctgattta aaactttctc tgattttatg ctgactccaa tacaaaatct tgtataattt
      841 atagcaattc ataaaagagg cttacaatgt tgcgtatcct tttgtatttt ctcttactca
      901 ttaacacaac attttgtctg gcaaagactg aaaaagccat ttttgcaggt ggatgttttt
      961 ggtgcatgga agcagacttt gaccatcttc ctggagtcct gtcaaccaca tcaggatttg
     1021 atggggacac tcttaaaaat ccaacttatg aacaagtttc tgccggtaat acccattacg
     1081 ccgaatcggt aatggtagag tttgatccca ataaattaag ttacaaaaaa ctggttgagt
     1141 atttctttat gcatattgat cctaccgtca aaaatgcgca attttgtgat cagggccgcc
     1201 aatatcgcag tgcaattttt tacatgaatg atcaacaaaa gaaaattgcc atggaataca
     1261 aaaaagagtt agagcaaaaa ttccccacaa tttatacaga aattgtccca tcaacttctt
     1321 tttatccggc agaagaatat catcaaaatt attacaaaaa aaatcctata cgttataagt
     1381 attatcgata tcgttgtggc cgggatgccc gagttcaaga ggtttggggt aataaaaaac
     1441 aagagaataa ttcttaatca tgatccatag ttttatcact tatcaaaaaa gaataagcaa
     1501 atcatgagaa aacgcgctaa tctaataaat aataattttt caatttttta ctaatacaat
     1561 tatgattact caagttaacc aattagatga acttcaatta aaagacttaa aagcattaag
     1621 agcagagtgt aaaaaaaatg atggcagcat ccccaatcta tatatccata tattgaaaca
     1681 acaccgctct cttcctacaa gcttccttta ttatcataac gggaccttga ttgggttctt
     1741 gagtgtttat ttcttttatg atgatgccgt tgaagttgct gttctggtta gtccccaata
     1801 tcgcaggcaa gggattgcta aacaattaat aaaggaagca ttaattttaa ttaagtctca
     1861 aaactacttt aacttgattt tttcatgccc ctcacgcctc aatgataact ggttaaccca
     1921 aaaaggattt acctacctgc atagcgaata tttcatggaa cgtgatgatt taaatcctat
     1981 actcgattac atgcgcccat tgtcctttcg catggcgact ttagaggata ttcctatatt
     2041 atgtggcctc gatgaagtgt gttttcctga taaaaaccaa gacttggtta accgattcca
     2101 acaaattctt aacgaaagag aatatgaaat tgtaatagcc atgctcaata atcatcccat
     2161 aggaaaagcc catattcgct ggcaaaccaa aagggctacc ttgtctgata ttgccatatt
     2221 gcctaaagaa caaggaaaag gatttggttc tgccttgatt gctcattgca tcaatatgat
     2281 tttgagcgaa ggcaaatccc gggtggatct tgacgttgaa acccataata aaaaagcatt
     2341 aaatctgtat attcagcttg gatttcatat tcaaaatgct tgtgattact ggtcaattaa
     2401 tgtgaatcaa cttgcaaaat aaaactgttt attctttcgt tacaaatcgc atattataaa
     2461 tatggcttga cataattaaa atctcaccat tagtattgcg aatggcgata gaggaaggct
     2521 tgttttatca aaaataagga taattaatga aaaaaagggt tgttattact ggtatggaaa
     2581 taacgtcatc cataggatcg ggaattgata aattttggct tgctgcatct caaggtcaat
     2641 gcggaattaa acgcatccaa tcttatgatc catcccccta ttcaacccaa atagcaggtg
     2701 agattacaga cttatctctt gaccatctcc ccgaatttaa caaaagcaaa cgttatcctc
     2761 gtgctgctca gtacgcatta ttttgcgcac atcatgccat tgagaaagca gggcttgagc
     2821 aagctgagct caatgcagca ggtaccttta taggcacaag cattggcggc caacctgaac
     2881 tggaagcagg ttacaagtta ttctttacag acagctggaa gaaaatcccc cctcttagcg
     2941 tgattagggg aatgcccaat tcaatagcca atcatatcgc aattgctttt ggcataggtg
     3001 gaccaaactc aaccatttcc aatgcctgtg tttcatccgg tgaagcgata ggcatggctt
     3061 accaacaaat tgcccatggc aatttgacca cagctctttg cggaggaaca gaatctttag
     3121 tctgggaatc ggttatggct gcctggtgta agcttagagt aatgtcaact aacaatgaaa
     3181 atccctcaca agccagcaga ccgtttgatc tcaatcgtga tggaatggtt atggcggatg
     3241 gggctgggat attaattctt gaagagctac aacaagcaaa agcaagggga gcacaaattc
     3301 tcggagagat cattggctat ggcgcaagtt gtgatgcatt ccatgtcaca gcaccaaact
     3361 cacaaggaca agcaagagct attgttgctg ctttaaatga tgccaagtta tcaattaacg
     3421 atgtccatta catcaacgca catgggacag gaacccagct caatgacagc acagaaacag
     3481 aaacgattaa aatggttttc ggtaaaagag cctatgacat cccgattact gctcaaaaag
     3541 ccatgacagg acatgcgatt ggggcagcag gggcaatgga aatcattgca acaacactaa
     3601 gcttgcaaaa aaatacgctg ttaccaacga ttaacctgca tactccagac ccagcctgtg
     3661 atctggatta tgtgcctaat gctgcgcgag aaaaaaccat tgatattgct ttatccaacc
     3721 atttcgcttt cggtggagca aatgccgctt tagttctgcg caaatacatt agttaagaat
     3781 ataaatagct tacgctaaac agcaattgat tattatataa atgggtcaat cgtggccctt
     3841 tggtaatatt aactccatca ccacccaaat aacttttccc taagtctgca aactcatatc
     3901 ctacaccaac ctgccagtta cgatttaaca ttttttggac accggccccc acactgtaag
     3961 caaaagcata agtagtgttt gacgcaaacc atggtgcagg aaacaggaat tgattgatag
     4021 aaagagtcct gtaatcatgg gcattattaa atgctattcc aaaactaccg ctcagataag
     4081 gttgcacggg tgtaaatgca cttcctatca gtttgccttt tagctctact cgtgcgtgat
     4141 tcactttata gtcatacgta tagacattaa ggaggccatt caggttgaca tcaccagtaa
     4201 tcttggcatc gcttactccc gctaccccta aacctaattg accaatcatt tggggattga
     4261 tcgcacgctg cagcccaaag aatatctcac cattggctaa aatagtcgtc ggagaatcat
     4321 gggtaaaata aagataataa tttggcggta aattgggatt gaaattatta atatagatcg
     4381 gagacattgg ataaatatac tgattttgtc caggtgttgc ccacgaaggt cctccactca
     4441 ctgtaataat agaggaccaa agtaagggaa tttcttcgtc ataaatcggt tctgaagcgg
     4501 aaagattcat actaaataaa acacataatc cggtgagcaa tatttttttc agcatatccc
     4561 atccttttga ttacaaatca gccctcttga ttcaggctct ttaatgaact gattttaata
     4621 gaagttattt gattttatac cctaattaat ctcatttttc tagggtgcgt tgacaattca
     4681 tctggatcac cagaatagca ggatcatgat aagaaaaatt attaatctga aagccatctg
     4741 aatcgaggat aattcactcg aaaatggaca ataagaggta tataatcgct ctattttgcg
     4801 gaattgaaag gtaaaccaat gaattcttct cttcgtgata tagcaaacct tgttcaaggt
     4861 atggttattg gcaatgatac catcactgtt tccacactat cacccataga taatatactt
     4921 cccggatctt tagttttcgc cgaaggtgaa gacaatatca aacttgctga aaactctgaa
     4981 gcagcagcta ttcttattgg tcaaggtact atcgattccc caaagccatt aattcaggta
     5041 aaaaaccctt ttaaagcatt tattgcctta ctcaaccatt tttatccccc aaagagaata
     5101 tctccggggg tacaccctac tgctgtaata ggtgcagaag tacaattagg tgatgaagtt
     5161 tatgttgggc cattcgtagt cattgaatca ggaagtatta taggcaaccg ctcggtgctc
     5221 aaaagccata ttcatatcgg ccataatgtg gtcattgggg atcatacaac cattcacccc
     5281 caagtcacta tttatgataa ttgccgtatc ggttcgaatg tcactatcca tgcctctaca
     5341 gtaataggtt ctgatggctt tgggtatact tttgttgatg gccaacatct caaagtacct
     5401 cattcggggt atgtagtaat tgaaaataat gtcgaaattg gagccaacac agcgatagac
     5461 aaggccacat taggtgctac agtgatcggt gaaggtacaa aaattgataa tttggtacaa
     5521 atcgctcact cagtcaaatt aggcaagcat aatattattt gtgcttttac tggcattgcc
     5581 ggcagtacca ctaccggaaa caatgtcatc tttgccgcca acgtgggagt gagtgaccat
     5641 gttcacatcg aggatgaggt catattaggc gcacgtacag gagttccccc acacaaacac
     5701 ttaaaaaaag ggacagtata tcttggtaat ccggcaaaac ctaaagatgt tgcgattaag
     5761 catgaactgt cagtgaatcg tatcccttta atacgaaaaa acattaaatc acttaccgag
     5821 caagtggctg taattaacaa aaaactcgat attaaagcaa aagaggtaga gtaactatga
     5881 aaccgcctct gattctaata gacggatctt cctatttctt tagagcgttt catgcccttc
     5941 caccattgac aaactccaaa ggccagccta caggcgcaat ttatggagtt gccaatatga
     6001 ttaaaaaaat aatcaaagat tatcaaccag aagaaatagc cgttgtcttt gatgccaaag
     6061 ggaaaacatt ccgagatgaa tggtatccag aatacaaggc acacagacca ccaatgcctc
     6121 aagaattgag ctctcagttt caaccgctaa tccagttact gcaagcaatg ggattgcccc
     6181 tgcttatcat cgatggcgtc gaagcagatg atgtgattgg cactttggca aaacaagcca
     6241 cagagcaagg cataccagtt gttatatcaa ccggtgataa agacatggca caattggtca
     6301 atgaacatgt ctccctcatc aataccatga gtaattacag tatggatatt gatggtgtca
     6361 aagcaaaatt tggtgtaaca cctgagcaaa tcattgatta cctcaccctc attggagaca
     6421 gtgtagataa tattccggga gtagagaaat gcggccccaa aacagctgta aaatggctga
     6481 cagaatatca aacgcttgat aatttgcttg cacacgccaa cgaaattggc ggcaaaattg
     6541 gtgaatattt gcgtgccagc atccctcatt tgccactctc aaaaaaattg gtcactatca
     6601 aaactgattt ggatttgccc ttaaatttaa accaattgat gcccaaaccg gctgatagcg
     6661 agcaattaat taaattaact cgtgaattag aatttaagtc atggcttaaa gagttgctac
     6721 aggaagaaga gaatcaaaaa tcacccaagg aaatggatgc cagcaataat aaatcctatg
     6781 aaataataac gactcatcaa cagttaaatc attggcttaa taaactagag caaagtcaac
     6841 aattttgcat cgacacggaa acaaccaacc ttgatgtcat gcaagctgaa ttggtaggca
     6901 tctctttagc agttgaggaa gaaaatgcat cttatattcc ccttgctcat actgatggca
     6961 gtacacagtt agtaaaagaa gaagttttaa ctgctttaaa gcctatttta gaaaatccag
     7021 ctataggaaa aatagggcaa aatattaaat atgattacag tgtccttaaa aattatggca
     7081 ttaccttaaa aggcattagg tacgacacca tgctggaatc ctatgtactc aatagtggtg
     7141 caggcagaca tgatatggat tctttggctt taaaatatct gggttataaa acaataagtt
     7201 atgaggatgt tgcgggaaag ggagccaaac aactccgttt tgaccaaatt ccagtagaga
     7261 aagctggagt ctatgcagcc gaagacgctg acattacttt gcgattacat cataaacttt
     7321 accccatgct ggatgagcca ttgcgtaacg tgttgcatga tatcgaaatg cctttactca
     7381 ctgttctcgc agacatggaa atgcacggcg ttctcatcga tccagtcatt ctggaaaagc
     7441 acggaagtcg tttgaaagaa caaatgaaat ctctggaaca ggaagcagtt caactcgctg
     7501 gaaaagcatt caatctcaat tccccaaaac aattacaaga aatactattt gatgaacaaa
     7561 aactgcctgt tgtagcgaaa acccccacag gacagccttc gactgcagaa tctgttttac
     7621 aggaattggc ttttgattat cgccttccgg ctgtaattct ggaataccga agcttatcaa
     7681 aattggtcag tacctatatt gatgccttac ccaaaagaat taaccccaaa acacataggg
     7741 tgcacacatc ctataatcaa gcggtagcag caacaggccg attatcatcc agtgatccta
     7801 atttacaaaa tatccctata cggagtgaag aaggaaggtt gattcgaact gccttcatag
     7861 caccagaagg aagtcttatc atggcggctg attactcgca aatcgagtta aggataatgg
     7921 cgcatttatc tcaagatgat aatcttttac gagcttttgc taatggttgg gacatccatg
     7981 ctgcaacagc gagtgaaatt tttcaaacga atttggaagc tgtgtccaaa gaacaacgaa
     8041 ggcgtgccaa ggcagtgaat tttggtttga tatatggtat gtctgccttt ggactggcaa
     8101 aacaaattgg tgtggagcgt caggatgctc aacattatat tgatacttat ttccgccgtt
     8161 atcctaaggt acttgaatac atggagcgta ctcgcaaaca agcgcatcaa cttggttatg
     8221 ttgaaacctt atttggtcgt cgtctctact tacctgaaat taattcacgc aacctaatga
     8281 gacaaaaagc agctgagcgt actgccatca acggaccaat gcaaggaacc gcagctgata
     8341 ttataaaaaa aaccatgctg gctatttcca gttgggagca ttcgcaaaag aacccctctg
     8401 caaagatgat catgcaggta catgatgaat tggtttttga aatccgaaag gaagcggttg
     8461 aagaatgtca cgccattatc cacaagctca tggagcaaac tgtaaaatta tctgtacctc
     8521 ttgttgtgtc tattggcaca ggttctaact gggatgatgc gcattgaaac gcataaaagc
     8581 gtttaattta ttggatttaa taataattaa tagaagtttg actcgcaaag gaaggatttt
     8641 gaccacctat aaactcacca aaataataag accaagtttt atgaatacgt tccaaaccaa
     8701 tgactttggg ccaagtatta tactcattca aaaacaggcc atgactctta aacactttaa
     8761 taaagtcttc tcgcatagca tcggaatcag accaggctac aataggaagt tgcgggtatt
     8821 tttgcaataa aattcctgca agctcgggac cgttgcagtt catttcatca gggctggcac
     8881 ccagatcacc atcaacaacg acgatatcgg ggcaatagtt attgacaaag gataatgcgg
     8941 cttgactatt attgacaata gtcactgaaa ttgatgcaac aaccgactcc aacaatcggc
     9001 gcaaacaaaa ggcattaaaa gcactgtcct caacaattaa tacacgcatc actgctccct
     9061 cttaactacc agagaagtat aatgtgcgaa ccttaagaaa atcttaagaa atagaattaa
     9121 tccagttaaa aattacaaat tgaaaaatca acgaagttcc ctattaaatt ttttttacaa
     9181 attgtgattt taatttcatg gcgcctatcc cgtcgatcct gcagtcaatg tcatgatcgc
     9241 cgtcaaccag acggatattt tttactttag ttcctacctt tatcacttga gaagagccct
     9301 ttatttttaa atccttgatg accgttatcg tatctccatt ttgcaacagg tttccattgg
     9361 catctttgat aatttttgca gactcttcgt cattgttatt actgtttttt ggccattcat
     9421 gcgtacactc agggcaaatt aatacatcac cgtcctcata gcagtattca gaaagacatt
     9481 tagggcaatt gggcaattgg ttcattttgt tgaaaaaccc tgaattaaat tgattacaaa
     9541 ctattttact ctaatagaaa gcattttaaa aatttttgac taaattgacc tgtaattgaa
     9601 cggccatgga atgaccggat aaataatgat ttttttaatc attatttatc caacgaaata
     9661 gaaatgctat gaattattga gtggattagt caatacgtat tcttcttggc attgctcttc
     9721 tttacctttc gtgggagttc ttcttgatag aacgcttggg aaatgaccaa ccggatgaat
     9781 attgtttgtg ctcagtaatt gagaaccacc caaaccccgc atagcctcat ggctgctttg
     9841 cttaacagca ggaacaggct cttcgcttac gggctctggg gtaatgtctg ttcctttagc
     9901 aactgcaaat ccaatagcac ttgatagtgc agcagcgcca gtaccactta tagcacctaa
     9961 agtcaaagct cctaaaagac caacacctga aggagcaaaa actccggtag caaccaatgt
    10021 aacacctaaa gcaaagccga aaccaaagcc aatgcctgca aaagcaccca taatctttag
    10081 ggtacgaaaa tcagaagacg gaaccggttg attagataag acagtcgccg tttcttcacg
    10141 ccgggataaa ttatcctcct gaagtaaagc aggggtctct tcaccgatag aaacctcatc
    10201 cccttgctca tatggtaatc gaacctcttc aggagagttc tctttaggcg ggaacatttc
    10261 cctaacatct tctaaatatc gggaccattc aaactcccca ggatttttac aatacccacg
    10321 aatttcttcc tcattgtatc ctctaccttt tactgcacgc agcatctcag catagtctgt
    10381 cgggctatca tcaaggatat aaacccggtt ggccctttct gttgggtgat taagctggga
    10441 attaacatca ttaaatttat accccgactc cagaatagtt aatttctcga tttcttcatc
    10501 ggaaaatcct tgatatttga aaactgcttt aatgtattca gctcggtttt tggtaacaat
    10561 attgacggtc acagcatcat tttttagtac ttcatggaaa aacgccactg cttcaggact
    10621 catcaggaaa tcaacatcct gctggtcatg atcaaaattt tcgttgtatt ttccatattt
    10681 tgcttcgaat aaagcctgag cttcaattgg atctttcatt ggagtgtttt tgtaatcttg
    10741 ctctacatcc tttttatagc cttgtaacag tgattgataa aactctgtaa atacagtttc
    10801 attaccgctt cttccggtta cagtaccatc aaaatcggta aaaataataa ctttaggcat
    10861 tgtgtttcct tactatttct cttaatttta aatgactcga tattgtacaa agtttaaatt
    10921 a
//
LOCUS       DASLOE010000101        10798 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.102, whole genome shotgun sequence.
ACCESSION   DASLOE010000101 DASLOE010000000
VERSION     DASLOE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10798
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.102"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            40..1236
                     /locus_tag="U6309_001999"
     CDS             40..1236
                     /locus_tag="U6309_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEN5505437.1"
                     /translation="MLEYLTWITANIVLVSNSIFGTPHPVHAQLQVPQCLAAKMTTAH
                     DVLAENEQFKIIDLPGSDIENVTLLAEQVNCGDFIDVTDKFSDTLLTAKRKSAKNILQ
                     SSALTALKKSQEDKNIYEIKHQKEVNAAIKEVVADNIWQTLNHLTSYSNRSATKDTGV
                     AAANWLKSQVDAMAMEYNRRDTATFLVKAGWYKQPSLVTVIGKELKTPAIVIGAPMDT
                     LDGNMPGADEGSGAAAIMESTRILLASKLKFKHPIYIIWYAASDRNLAGSQYVVQYFQ
                     EKSIPVKAVLQLNKTGFRANSDDSTMWVFTDNTNNDLNQYIAKLIKNYIHTPVNFSQC
                     NYECGDHISWSQENIPVTYAVESDFKNLNPYIGSSSDTMDFLNLEHIANFSKLAIAFS
                     IELGSK"
     gene            complement(1294..2973)
                     /locus_tag="U6309_002000"
     CDS             complement(1294..2973)
                     /locus_tag="U6309_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate decarboxylase"
                     /protein_id="HEN5505438.1"
                     /translation="MCSIGEYLAKRLEELNISEYFAIPGDYNLILLDEVLKNEKIKMI
                     NCCNELNAGYAADGYARVKGVSALFVTYSVGGLSVVNAVAGAYAENLPILVISGGPNT
                     NSIQDAEILHHTLATEDNLYVRDIFSRITAHVVCIRKPEQAAMQIDEAIAIAIAKKKP
                     VYIEIACNIANYPISKPTQRAFNTYKISDSSSLAAAVEHAAEKLNSALKPSLIVGSKV
                     RHCNAINSVSELAKCTGYAMAAMPDAKGFISEQHPNFIGIYWGPVSSSGCAEIIDSSD
                     AYLLIGPNENDYTTVGYVWGINPHKSIKTTKGGVIIGETVYTNIFMNDFLRELSKKLK
                     FNDASYKAYKRIAEETVFYPELDQPDAPLSSRYLFGQIQKMLSDKKALLAETGDSWFN
                     CMRLSLPEGCPFEIQMQYGSIGWSVGALLGMQAALHNQKRVIACIGDGSFQMTAQEIS
                     TMIRYGYKPIIFLMNNACYTIEVQIHDGPYNVINNWRYAELVNVFRGEQSNAKSFVVK
                     TNKELLTAIKQVEKTDALCFIEVFLDKDDCNKNLLEWGSRVANYNSRPPRR"
     gene            complement(3213..4295)
                     /gene="ravQ"
                     /locus_tag="U6309_002001"
     CDS             complement(3213..4295)
                     /gene="ravQ"
                     /locus_tag="U6309_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavQ"
                     /protein_id="HEN5505439.1"
                     /translation="MMRMFSVRRNPSAVMRKDLSLTSRPLLQAPLLKSTVNPQNTLKN
                     KELKERKFTETLPDSRFFSKLKLAEQDQFSHRKRKRFNYQKNFSAALSLPYSRKELDV
                     HRDEEDTLLSMVKKYVIEHANRRVDLFFYTLIAYYNTEIVPTNGYTDLQHGRGRNTTR
                     GRTITEACHSSLIPSLLDSTIYQNKGRLKHKSLLSGTHFMDSLNATVELPPFVNDFDD
                     ILEKTCRKKCLKILHEVSLGRINPMQGLTLFLKMMQDILIKFKEEAASENQSVLLQNS
                     LIRKKFISPNLVDLVINGTLLTTFSDSTQSASDDYIQLLLRMTSEEKALCEKDEKGKI
                     KLYLAKIMEIQNEILTSKNGEFVPSL"
     gene            4781..6268
                     /locus_tag="U6309_002002"
     CDS             4781..6268
                     /locus_tag="U6309_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN5505440.1"
                     /translation="MQNKLLQFICIKKLCKQKKSYKSIIKFASLLLAKKQIICAFALV
                     SIIKSSWAAHHYDTIIIGAGVSGLTAAYHLHTAQQKVLIIEAKNRLGGRVYTSYDWGF
                     ATDLGASWIHAIENNPLLPLIGKQSIIINSYSNSDPVAMLTNYALYDSEGKPVSKQTQ
                     TLFSSLTKEFLRYCQTRSQMISFAQNLTTFAKQKKLTSKQLALLSYALENIYTYEFAD
                     NLTKLSRNVHSASEASIASGKNALVPEGYFQLFRPLTQHVPIHLNQIVSQINYGPDGV
                     NIITQHEKYHAKQVIITVPLGVLKANTIKFHPALPKDKRTAISQLGMGSYEKLYLLFD
                     KVFWDKDKEWIGMLPQNEQEAFNIFNYYKYTKKPVLIVFTSGKLARDMEKEHLTEWVM
                     QHLRRIYGNNIPKPIKNKKTHWGSDPFTRGSYSYLPVNVDKSVIGTLAQPVANRLYFA
                     GEATSTTDPSTVHGAYLSGIRAAEEVLASIKHSGKNRERNKAVNS"
     gene            6168..7118
                     /gene="ravP"
                     /locus_tag="U6309_002003"
     CDS             6168..7118
                     /gene="ravP"
                     /locus_tag="U6309_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavP"
                     /protein_id="HEN5505441.1"
                     /translation="MALIYQGYAQQKKYWPASNTLEKIEKEIKLSIHKPILILEKVMY
                     TKRDNHLLINKEEEQEENQKTVFVPQTNNLALPETQSYFCTAEQCSLNNPYAPAQGYT
                     RPDSLHFKTAPINSEIYEQVFKVSEETVKTETQSANRVPGHLEEEENRSGDWSGLIPD
                     ELYQMMMEAAKQGIFVSFLLTLTDEIINDYLKNHHYSQQQINWISQGIRALTLIAIGT
                     SPGIALGLPISTLLLTEQLGFDKTNVNYLTTSSVVAWGLLSSPFNLLNTSLVMASSIG
                     ASVLTSKITQSSYQFVRNRFFSSKPEKEELEQENILQKTI"
     gene            7329..8801
                     /locus_tag="U6309_002004"
     CDS             7329..8801
                     /locus_tag="U6309_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505442.1"
                     /translation="MAHAKTFFSQQAHSSLTLVNYVHHDGIGDFRHLLDFVFYFNNTP
                     FLKEIELICIVINGHKPGNPQSVYIEEQLENMSVAHKILIHDSINGHENLRQEIKKNR
                     PLRKQLQHTIGIIDIALNTSYKLVIQEFCKDKPPTITIAEIDLYATYDQPSLTSDPEK
                     RIDKSRYQADHVYIMGLGKGKVGLKLTGGLFNSPLKAREALQQLSDKRIKNLLLGQVD
                     EDPISQETINAFLQNNHFIPGYLQDIDTIFGFISIFATNHKLNAERKNLIFYLNNYQN
                     LFKKNYYDEDTGRRYSQFTDLLFDKHFPWHNVFANSGIRSITIYNYSGQKPTEEEITL
                     NLEGQQITIITGIMLNDTDYQLLYDIPQHFVAASGDNTLENAINHGLLFLMQYKYQYE
                     TVREIAKIAERVKDTLGFNEEEIREFHAYFGEAHHLISYSNEKSTKARSLLQSIDLIR
                     LSSNMRKICAYLIEKENFLHQLPEILQTDLLNISKPGMKQ"
     gene            complement(8878..9942)
                     /locus_tag="U6309_002005"
     CDS             complement(8878..9942)
                     /locus_tag="U6309_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair protein Ada"
                     /protein_id="HEN5505443.1"
                     /translation="MFKINDHKKKEYYTALLSKNTCYEGIFYVGVKTTGIFCRPTCPA
                     RKPKLENCEFFHTAQEALLASYRPCRRCQPLSHPNQVSDLVRHLVDAVEANPEKRWKD
                     SDFAALSVDVATARRQFKKRFGMTFVAYARARRMGMAMKQIREGDLVINAQIDAGYHS
                     GSGFRDAFAKIMGAAPSHFNEQHKILKASWLDTPLGPMLAVADEHALYLLEFVDRRGL
                     EREVERLRNKTKAVIIPGDSQILKAIHEELELYFSGELRQFNTPIHLIGSPFQEKVWL
                     SLIGIPYGETRSYLQQAEVIGNSSAYRAVANANGANQLAIVVPCHRIINANGELGGYG
                     GGVNRKQWLLEHERRHIRGC"
     gene            complement(10186..>10798)
                     /locus_tag="U6309_002006"
     CDS             complement(10186..>10798)
                     /locus_tag="U6309_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505444.1"
                     /translation="QLGWVSFDHALINYPYLLLVAGLLYLAFFPFLDGLYQMIKAHLD
                     GKPINMNTEDIDFINPGTSKVMGMSMGPILLTIIIMENVGSVFKPFQVLVLLAYVLAI
                     FTPILTVNNPQSPLKKGFECIQFALANKKLVSKVLGLRLLILLFLFFPWIIIGITGEH
                     VILKVITIMAGVPLFFYSLVKLLPFYFFYPAYVYHQAKHQSMQ"
BASE COUNT         3559 a         2131 c         1852 g         3256 t
ORIGIN      
        1 atgaatcgat acggattcta cagttgttta ggagtttaaa tgcttgaata tttgacatgg
       61 attactgcaa atattgtatt agtgagtaat tcaattttcg gaacaccaca tccagttcat
      121 gctcaattac aagtacctca atgtcttgct gcaaaaatga cgactgcgca tgatgtcctg
      181 gctgaaaatg agcaattcaa aatcattgat ctgcctggca gcgatattga aaatgtaact
      241 cttttagcag aacaagtgaa ttgtggcgat tttatcgacg taacggataa gttctcggat
      301 accctgttga ccgccaaaag aaagtcagca aaaaatattc tgcaaagtag cgcattaaca
      361 gcccttaaaa aatcccaaga agacaagaac atctatgaga ttaaacacca aaaggaagtc
      421 aatgctgcaa taaaagaggt agttgcagac aacatttggc aaacattaaa tcacttgacc
      481 tcctatagta atcgttcagc aaccaaggac acaggagtag ctgccgccaa ttggttaaaa
      541 tcccaggtgg atgcaatggc aatggagtac aatcgcagag atactgccac attccttgtg
      601 aaagcaggtt ggtataaaca accctccctg gtgacagtca ttggtaaaga actgaaaaca
      661 cccgccatag tcattggtgc gccaatggat actttagatg ggaacatgcc tggcgctgat
      721 gaaggtagtg gtgctgctgc cattatggaa agcactcgta ttttgttagc ctccaagctg
      781 aaattcaagc atccgattta tattatctgg tacgctgcca gcgatagaaa tttggcaggg
      841 tctcaatatg tagtccaata ttttcaagaa aaatctattc ctgttaaagc cgtactgcaa
      901 ctcaacaaga cagggtttcg cgctaactca gatgattcaa ccatgtgggt atttaccgac
      961 aatacaaaca atgatctgaa ccaatatatt gcaaaattaa taaaaaatta catccataca
     1021 ccagttaatt tttcgcagtg taattacgaa tgcggtgatc atatttcctg gagtcaagaa
     1081 aatatacccg taacctacgc agttgaatct gactttaaaa accttaaccc ctatatcggc
     1141 agctcttcag atacaatgga ttttttaaat ctggaacata tagctaactt ttcaaagctg
     1201 gccattgcct tctcaataga gctgggctcc aaataaaaac acgtcaaatc attaatatca
     1261 gggtaggcga ttcttataat tcctacccaa gatctatcta cgaggaggac ggctattata
     1321 atttgccact cttgagcccc attccaacaa gtttttattg caatcatcct tgtccaggaa
     1381 cacttcaata aaacaaagcg cgtctgtttt ttctacctgt ttaattgcag tgagtagttc
     1441 cttgttggtt ttaactacaa aagattttgc attactctgt tccccgcgga atacattaac
     1501 cagttcagcg taacgccaat tattaatgac attataagga ccatcatgaa tttgtacttc
     1561 aatagtgtaa caagcattat tcattaaaaa aataattggt ttatacccgt aacgtatcat
     1621 ggttgatatt tcctgggcag tcatttgaaa tgagccatct cctatgcagg caatcactct
     1681 tttttgatta tgcaaagcag cttgcatgcc tagcaaagca cctactgacc aaccaataga
     1741 accatactgc atttgaattt caaaaggaca accttcaggc aaagacaggc gcatacaatt
     1801 aaaccaggag tcccctgttt cagccaacaa agcttttttg tcagaaagca ttttctgaat
     1861 ttggccaaac aaataacggc tgcttaatgg agcgtctggt tgatctaact ctggataaaa
     1921 gacagtttct tcagcaattc gtttataagc tttgtatgaa gcatcattaa attttaattt
     1981 tttacttaat tctctgagaa aatcattcat gaagatattg gtataaactg tttcaccaat
     2041 aataacccct cccttggtag tctttattga tttgtggggg ttgattcccc atacataccc
     2101 aactgtagta tagtcattct cattaggccc aataagcaga taggcatcac tggaatcgat
     2161 aatttcggcg catccagaag agctgactgg tccccaatag atacctataa agttaggatg
     2221 ctgttctgag ataaatcctt tcgcatcggg catagccgcc atagcatatc cagtacactt
     2281 tgccaactcg ctgacagaat tgattgcatt acaatgccta accttactac ctacaatcaa
     2341 gctgggcttt agagcggagt ttaatttttc agcagcatgc tctactgctg cagctagaga
     2401 cgaactgtcg ctaattttat aggtgttgaa tgcgcgttga gtaggcttgg agataggata
     2461 gttagcaata ttacacgcta tttcaatata gaccggcttt tttttagcga ttgcgattgc
     2521 tatagcctcg tctatctgca tggcagcctg ctctggcttt cgaatacaaa cgacatgggc
     2581 agtaattcga gaaaaaatgt cccttacata caaattatct tcagtcgcca gcgtatgatg
     2641 gagaatttca gcatcctgta tggaattagt atttgggcca cccgatataa ccaaaatggg
     2701 gagattttcg gcatacgctc ctgcaactgc attaacaaca ctaagtccac caacactata
     2761 agtaacgaat aaagcggaga ccccttttac ccgagcatat ccatcagcag cgtaccctgc
     2821 attcaactca ttacagcaat taatcatttt tattttttcg tttttcagaa cctcatcgag
     2881 taaaataaga ttgtaatcac ccggtatagc aaaatattca gaaatattta attcctccag
     2941 ccgctttgcc aaatactctc caattgagca catgttaatc ccttattaaa taagaacttt
     3001 ttaaattata gcacagccaa aaatcagtag atggcaagtg aatacctgct agaaaataga
     3061 gttcacaagg catcaaattg caaatgaaaa tttaaatatt ctatcttgga gtctgttgac
     3121 aattcgaccc agaaactaca tcccgcgtct tgggcgcggg attaatggat gctgtggaca
     3181 aacaaacagg tcggcatttt tgttaacaca tcttaaaggc taggcacaaa ttcaccattt
     3241 ttcgatgtca aaatctcatt ttgaatttcc attatttttg ccaaatacaa tttgattttg
     3301 cccttttcat ccttttcaca aagcgccttt tcttctgatg tcattctaag taaaagttgg
     3361 atgtaatcat cagaagcgct ttgtgtgcta tccgaaaatg tggttaataa ggtgccatta
     3421 ataaccaaat caaccaagtt tgggctgatg aattttttcc ttatcaatga attctgcaat
     3481 agtacagatt gattttcgct tgctgcttct tctttaaatt ttattagaat atcctgcatc
     3541 atttttagga aaagggttaa tccttgcatt ggattgattc ttccaagtga tacttcatgc
     3601 aggattttca agcatttttt cctgcaagtc ttttccagga tatcatcgaa atcattcaca
     3661 aagggaggta attctacagt ggcatttaga ctgtccatga agtgagttcc ggaaagaaga
     3721 cttttatgtt ttaatcttcc cttattttga tagatagtac tatcaagcaa tgatggaata
     3781 agactgctat ggcaggcttc agtgatagtt ctgcccctgg tggtattacg ccccctgcca
     3841 tgctgaagat cggtataccc atttgttgga acgatttctg tattataata agctatcaat
     3901 gtataaaaaa ataagtcaac acgacgatta gcatgttcaa tgacatactt tttcaccatc
     3961 gacagcaatg tgtcttcttc atcccgatgt acatctaact cttttcttga gtatggcagt
     4021 gagagtgctg cactaaaatt cttttgataa ttaaatcgtt tacgttttct gtgactaaat
     4081 tgatcctgtt ctgccaattt taattttgaa aaaaaccttg aatccggtaa tgtttcagta
     4141 aatttacgct cttttaactc tttatttttc agagtgtttt gagggttaac agtactctta
     4201 agcagcggcg cctggagtaa agggcgggaa gttaaagaaa gatctttgcg cattactgcg
     4261 gaagggtttc tacgaacact aaacattctc atcatggtct tctcccttaa tggccattat
     4321 gtcaccgtag tacctgctca aagcatcaag cgtctgtttt tccataaaga cacttaaact
     4381 tacttagtga gagttcacag tctggatgat cttcaaagcc agctccctgt ctaagccaga
     4441 acatatactg tctttttaca gtacataata atctttaaaa aagcaaacat taaaataact
     4501 taatattgaa tgcttcatac agacaagtta attgctatgc tgattattat ttacaaatat
     4561 tgatactttt tttaatatgc atcaattcaa taacaaggat ttactgtgtc ataccagact
     4621 tttttctcga cttgatcgtc ataattcaat cttgtgcact cgcatgcagc caagcaaata
     4681 caaaattcta gccagtcaag gttagatgcg aagcttggtt gggcaaatca atttgtccgg
     4741 ctaaatctat aaattaaaat agcgatgtag gaagaaaaac atgcaaaaca aattattaca
     4801 gtttatttgc atcaaaaaat tgtgcaaaca gaaaaagtct tataaatcaa taataaaatt
     4861 tgccagtttg cttttagcaa agaaacaaat tatctgtgcg ttcgcattag tctctattat
     4921 aaaatcgtct tgggccgctc atcattatga cacaataatt attggtgctg gagtatccgg
     4981 gttaactgca gcgtatcatt tacataccgc acagcaaaaa gtacttatta ttgaagcaaa
     5041 aaaccgtctt ggaggcaggg tatatacaag ttatgattgg ggtttcgcca ctgatttggg
     5101 agcatcctgg attcatgcca ttgaaaataa ccctttactg ccgttgattg gtaaacagtc
     5161 aattattatc aatagttaca gtaactctga tccagtagcc atgctgacca attatgcctt
     5221 atatgacagc gaaggaaagc cagtatcaaa acaaacgcaa actttatttt caagtttaac
     5281 caaagaattc ttaagatatt gccaaactcg cagtcaaatg atctctttcg cgcaaaatct
     5341 cactaccttt gcaaaacaaa aaaaacttac ttctaaacaa cttgccttac tgagttatgc
     5401 cttggaaaat atctacactt acgaatttgc tgataatttg actaaattat cacgtaatgt
     5461 ccattccgca tccgaggcat ccatagcttc tggaaaaaat gcccttgtac ctgaaggcta
     5521 tttccaactt ttccgcccac ttactcagca tgttcctatt catttaaacc aaatagttag
     5581 ccaaattaat tacggacctg atggtgtcaa tatcataact caacatgaaa aatatcatgc
     5641 caaacaagtc attattaccg tcccattagg cgtgctcaaa gccaatacaa tcaaatttca
     5701 tcctgcttta cccaaagata agcgcacagc aatctctcag ttagggatgg gaagttatga
     5761 aaaattgtac ttattatttg acaaggtttt ttgggataaa gacaaggaat ggattggcat
     5821 gctcccccaa aatgagcaag aagcattcaa tatctttaat tattacaagt acacaaaaaa
     5881 gcctgtactc atcgtattta ccagtggcaa acttgcgcgc gatatggaaa aagaacattt
     5941 aaccgaatgg gtgatgcaac atttaagacg aatttatgga aacaacatcc cgaaaccaat
     6001 taaaaataaa aaaactcatt ggggcagtga tccattcaca cgcggcagtt attcttactt
     6061 gccggtaaac gttgataaga gtgttattgg cactctcgct caacctgtag ctaaccgtct
     6121 ctattttgct ggagaagcta cctcaaccac agatcccagt actgtgcatg gcgcttatct
     6181 atcagggata cgcgcagcag aagaagtact ggccagcatc aaacactctg gaaaaaatag
     6241 agaaagaaat aaagctgtca attcataaac ccatcttgat tttggagaaa gtaatgtaca
     6301 ctaaaagaga taatcatttg ctgattaata aagaggaaga acaagaagaa aaccaaaaaa
     6361 cggtatttgt ccctcaaaca aataatttgg ctcttcctga aacccaaagc tacttttgta
     6421 cagcagagca atgctcttta aataacccct atgccccagc tcaaggttat acaaggcctg
     6481 attcattgca tttcaaaaca gcccctataa actcagagat ttacgaacaa gtatttaaag
     6541 tttcagaaga aactgttaaa accgaaacac aatctgcaaa tagagttcct ggacacttgg
     6601 aagaagagga aaatcgttcc ggtgattgga gtgggcttat tccggatgag ttatatcaaa
     6661 tgatgatgga ggctgcaaaa caaggtattt ttgtctcgtt cctgctcact ctcactgatg
     6721 aaataataaa tgactatcta aaaaatcatc attattcaca gcaacagata aattggattt
     6781 ctcaaggcat tcgagcttta actttaattg ctattggtac ttcaccagga atcgctttag
     6841 gattaccgat aagcacactt ttactgacag agcagttggg ttttgataaa acgaatgtta
     6901 attatctcac aacaagttca gttgtagctt gggggctatt aagctcgcct tttaatttgc
     6961 ttaatacctc ccttgtgatg gcaagttcta ttggagccag tgtattaaca agtaaaataa
     7021 cacaatcatc ctatcaattt gtgcgtaacc gttttttttc ttcaaaaccc gagaaagaag
     7081 agttggaaca agagaacata ttgcaaaaaa caatctagta ataattcacc gtaataactt
     7141 tatgaattac tatgagattc ctgctaaaag gtctcatctc ttcacgtgct ttgggaaaaa
     7201 taatcagaga acacttcaga tttcataacc aaaattctca agcatagaac caaaagattt
     7261 taaattagtt gatatttact aactaatatt gactatattt actgtatgat tactttttgt
     7321 gcataataat ggcacacgct aaaactttct tttctcaaca agcacactct agccttactc
     7381 tggttaatta tgtacatcac gatgggattg gtgatttcag gcatttactc gattttgtgt
     7441 tttattttaa caacactccg tttttaaaag agatcgaatt gatttgtatt gttataaacg
     7501 gtcacaagcc gggcaatccc caatctgtgt atatagaaga gcagttggaa aacatgagtg
     7561 ttgcacacaa gattttgatc catgactcca tcaatggtca tgagaatttg cgacaagaaa
     7621 ttaaaaaaaa tagaccatta cgaaaacaat tacagcatac tattggaatc attgacattg
     7681 cattgaatac ctcttataaa ctggttatcc aggagttttg taaagacaaa ccaccaacaa
     7741 tcactatagc tgaaattgat ttatacgcta cttatgacca accatctcta acttcagatc
     7801 ctgaaaaacg tatcgataaa tcaagatatc aagctgatca cgtttacata atggggctgg
     7861 ggaaaggaaa ggtagggcta aaattaacag ggggattatt taactctcct ctaaaagccc
     7921 gagaagcttt acagcaactc agtgacaaga gaattaaaaa tttattgtta ggtcaagttg
     7981 atgaagaccc catttcacaa gaaacaataa acgctttttt acaaaataat cattttattc
     8041 ctggttacct tcaggatatc gatacaatat ttggattcat cagtattttt gccactaatc
     8101 ataaattaaa tgcggaaaga aaaaatctca ttttttattt aaacaattat caaaatctat
     8161 ttaagaaaaa ttattatgat gaagacacag ggagacgtta ctcacagttc accgatttat
     8221 tgtttgacaa acatttccct tggcataacg tttttgcaaa ttcaggtatc cgctcaatta
     8281 ccatctataa ctattcaggt caaaagccta ctgaagaaga aataacctta aatctggaag
     8341 gtcagcaaat aactatcatt actggtatta tgcttaatga tactgactat caattgctct
     8401 atgatatacc gcaacatttc gttgcagctt caggtgataa tacgctggaa aatgctatta
     8461 atcatggtct gttattttta atgcaatata agtatcaata tgaaaccgtt agagaaattg
     8521 ctaaaattgc agaacgcgtt aaagatacct taggttttaa tgaagaagaa attcgagaat
     8581 ttcatgccta ttttggtgag gctcatcacc ttatcagtta tagtaatgaa aagtccacaa
     8641 aagccagatc tttgttacaa tcaatagatc taatacgtct gtcttccaac atgagaaaaa
     8701 tttgtgccta tttaatagag aaagagaatt ttttgcatca attacctgaa attttacaaa
     8761 ccgatttatt gaacataagc aaaccaggaa tgaaacaata aattgcatca atcactacag
     8821 ctactggtag attaacatgt ttattctata ctttttttgt gcgtaaacaa aattagtcta
     8881 gcaacctcga atatgccttc tttcatgctc aagtaaccac tgtttacggt tcacaccacc
     8941 cccatatccc cccaactcgc cattagcatt gataatccga tgacaaggaa ccactatagc
     9001 taattgatta gcgccattgg cattagccac cgccctgtaa gccgaagaat tacctattac
     9061 ttctgcctgt tgcaggtaac ttcttgtttc accataggga ataccgatta atgagagcca
     9121 taccttttct tgaaaagggc ttccaattaa atggataggc gtattaaatt gtcttagttc
     9181 tccagaaaaa taaagctcca attcttcatg tatagccttt aagatttgag aatctcccgg
     9241 gataattact gctttggttt tatttcttag cctttcaact tctctttcca accctcttct
     9301 atcgacgaat tcaagtaaat ataaagcgtg ttcatccgca acagcaagca tagggcctaa
     9361 aggtgtatct aaccaggaag ctttaaggat tttgtgctgt tcattaaaat gagagggagc
     9421 tgctcccata atcttcgcaa acgcatctct gaaaccactt cctgagtgat atccagcatc
     9481 aatttgtgca ttaataacta aatcaccttc tctgatttgt ttcattgcca tacccattct
     9541 tctagcacga gcataagcta caaatgtcat tccaaatctt ttcttaaatt ggcgccttgc
     9601 tgttgcaaca tcaactgata aggctgcaaa atcactgtct ttccatcttt tttcagggtt
     9661 agcctcaact gcatcaacta aatgtctgac caaatcagat acttgatttg gatgtgacag
     9721 aggttgacag cgtctacaag gtctgtaaga cgctaataga gcctcttgtg cggtatgaaa
     9781 aaattcacaa ttttccaatt ttggttttct tgcaggacaa gttggtcgac aaaaaatacc
     9841 tgttgttttc actccgacat aaaaaattcc ttcatagcag gtatttttac tcagcagagc
     9901 agtataatat tccttttttt tgtgatcgtt aattttaaac atccccattc ctaaaaagcc
     9961 ttcttattaa ttattataaa tcttctttga aatggcgaag ccgaaaaaca gacagtgaat
    10021 tttttaaatt agataggact tttcatgaaa cttctcttat cctaatagtt cttttggcta
    10081 acaaagaata gatgccactt ttaaataagc agtcaaatac cggcataatc tactcttgat
    10141 atcgcaaaaa agtagagtcc tttcatgaga aaatgagcaa ttaagctact gcatgctttg
    10201 atgttttgcc tgatgataaa cataggctgg ataaaagaag taaaaaggca ataatttaac
    10261 taatgaataa aaaaataaag gcactcccgc catgatggta ataaccttta aaatgacatg
    10321 ctcaccagta atccctataa taatccaggg aaaaaacaag aatagaagaa tcaaaagacg
    10381 taatcccaaa accttactta ccaatttttt attggcaaga gcaaattgta tgcattcgaa
    10441 tccctttttc aaaggtgatt gcgggttgtt gacagttaaa ataggcgtaa aaatagctag
    10501 cacataagct aacagcacca atacttgaaa aggtttaaag acagaaccaa cgttctccat
    10561 gataataata gttaataaaa tggggcccat agacatgccc attactttgg aagtaccagg
    10621 gttaataaaa tcgatatcct cagtattcat gttgataggc ttgccatcca gatgggcttt
    10681 aatcatctgg tatagtccat caagaaaggg gaaaaaagcc aaataaagta accctgctac
    10741 tagcaaaaga taaggataat taatcaatgc atggtcaaaa ctaacccaac ccaactga
//
LOCUS       DASLOE010000102        10699 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.103, whole genome shotgun sequence.
ACCESSION   DASLOE010000102 DASLOE010000000
VERSION     DASLOE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10699
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.103"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..576
                     /locus_tag="U6309_002007"
     CDS             <1..576
                     /locus_tag="U6309_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505445.1"
                     /translation="FSKTIPLTSQEAFEILCTRDYLEKISKIIFNFQQLFNVKRSTLL
                     SHHKLNPKISNNREFLQDLEARYDRLNHAVQNNEPYPFLYGDVCLLKEYLQVILGYYQ
                     EQLKEEQPVAKNNLRRIRGSHKFPTLMSDISKGEHPELGKEDSEILIKYTINFCAEST
                     MWNDVKTISDLVIKPFLFDHKDEQDFSYCNP"
     gene            812..1573
                     /locus_tag="U6309_002008"
     CDS             812..1573
                     /locus_tag="U6309_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN5505446.1"
                     /translation="MKRIVFILLFISNVLVHSESLKVGVLQFAPPFSSISDKANHYYG
                     FVVALMNIICNRLQEKCEYIPIPKEGELKGLDKGVYHIVFSSTPITTSLPANYRYSLP
                     YLPSDGQFLTLKNNSIDTLNDINFKKVGFFKINFQQSPLLKKYETTNTLIEYTDPAEL
                     VNALTTKKIDAILINYQASRFIVNNLNPTEDDQLKLIGDKIPIGSGYAIIALKNQSAL
                     IDKINHILLEMEKDGSYLRIYNEYFGANPAYNLHH"
     gene            complement(1916..3244)
                     /locus_tag="U6309_002009"
     CDS             complement(1916..3244)
                     /locus_tag="U6309_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEN5505447.1"
                     /translation="MIKTFIDKKIQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKM
                     NHEAYRPEVQDQIRWLLDHPGFVTKACKQAEPYLYHVTREIQRRNLPGELALLPMIES
                     AYDPFAYSKVGAAGLWQIMPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVF
                     NGSWILSIAAYDAGEGAIGRAVKANNTNGGSVSFWNLDVPKETQIYVPRFLALAEIMS
                     NPRAYKINLPPMPFRPYFEEVNIGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPY
                     KPFTLLIPIAKVTQFYFNLSHSPVDKRVSWRKHLVLPSDSLQSIARKYHTTVSLIKKL
                     NRLSDHSIRPNQILLIPSAKSTPALPFHEISKSKFLSQTPTKGKVYRVIHIVQANETY
                     NTLERLYKVTAQDIMKWNHIGLGEALVKGRQLIIWKKSSNLEWNRTFKKP"
     gene            3527..3907
                     /locus_tag="U6309_002010"
     CDS             3527..3907
                     /locus_tag="U6309_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505448.1"
                     /translation="MEMGWLVVCIKHQGMIRINIFRRLEIMGTFFEWFPREVGLDKNP
                     DAFRTDCYQYYWGEPPRWGGTVDACEVETDLYAMVYNSIGLLSYLIPLVGGYLALHFA
                     RNANSKEFSEDNPEHGLEQSVLSP"
     gene            complement(4109..4879)
                     /gene="ceg19"
                     /locus_tag="U6309_002011"
     CDS             complement(4109..4879)
                     /gene="ceg19"
                     /locus_tag="U6309_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg19"
                     /protein_id="HEN5505449.1"
                     /translation="MFKKIITKLGIVMSKLRALEKEANELDLILDQALLSQSIEVKRR
                     ALSQFENSELIQNKDNLRVFIDPEHPIVSLIILATKTLPEKIENLQKKVRLLEIQLAA
                     ETEETLDESNSDSESVDYDNGINEETVVPVTVTHQEHVSEPDARSVAIARLKEALKPY
                     IDSTQERTYEHYYGMAGTLFSFFGAAGYSKTDKLNAITKLFQNLEVDGAEPLNDTDRD
                     ILTTGVLGATMKPLLEDEHIGNELKDLLSIKTDNVNTI"
     gene            complement(5040..6104)
                     /locus_tag="U6309_002012"
     CDS             complement(5040..6104)
                     /locus_tag="U6309_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HEN5505450.1"
                     /translation="MVNKICLSFALLISVPSILLAQDKLIFAVDIIRHGDRTPIVALP
                     TVNYQWQEGLGQLTAEGMQQEYKMGVAFRKKYIEESHLLPEHYEYGTIYVRSTDYART
                     LMSAQSLLMGLYPPGTGPTIPAGTSALPHAFQPIPVFSAPSKYDEVIIQQVDRKEREK
                     LMEQYVFSTREWQQKNNELKDKYPLWSRLTGINIDNLEDLETVGHTLYIHQIHNAPMP
                     EGLASNDIETIINSAEWAFMAQEKPQQIANVYSSKLMTNIVDYLNSGSMKKSKLKYVL
                     LSAHDTTIASVLSFLGAPLEKSPPYASNVNFSLYDNGANYYTVKITYNGNPVLIPACG
                     GSVCELQQLINLVNDSKNSV"
     gene            6372..7487
                     /locus_tag="U6309_002013"
     CDS             6372..7487
                     /locus_tag="U6309_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN5505451.1"
                     /translation="MSRYVCKWKLIYTFFIFIVFNNCPLAFASPSLTKQLHTILKDNL
                     ADSLTPGAVLLISSPDLGTISVAAGLADKENDISLNITDSFRLASMSKTFLAVTVLKL
                     VADKQLNLDDHIADLLPDSIDIERIPNGDEVTVRQLLQMRSGIPNYTDYDSYSDLIDT
                     MADKEWTPEECIKLVYDQKPDFKPGTAYEYSNTNYLLLQVIIENITGESYAESVKKYI
                     LKPLRLKNTYIEKQEFDEDHHYLSTHGYTLEDGKVVDVTDHDDGFGLADGGIISTAED
                     INIFVQALLKDKILLSPNYLTMMLTFPDDYGFGIGREEINGEIAWSHNGASSGYQGQY
                     YYFPERQLTVVILTNYFDSDIIEDLVSQTLSAIDNSE"
     gene            7579..8052
                     /locus_tag="U6309_002014"
     CDS             7579..8052
                     /locus_tag="U6309_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505452.1"
                     /translation="MTIYKFEISTMQNGNIQISAKHKSTGEIHGYVQIASSARLESRN
                     LLEIDQLYVDNNARGHGLGSELASRAIKYALKMDKGIHMMIQPGSDAFWKKYFNSRFD
                     GKRITLFGGETLNTNLADGKVFEISKESLVANYKFPQDRHLRDRDMEKICAKLSH"
     gene            complement(8162..10516)
                     /locus_tag="U6309_002015"
     CDS             complement(8162..10516)
                     /locus_tag="U6309_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505453.1"
                     /translation="MRYSLLLLGALTAIMTSVSANTNTSLKPTIAQPTTCVNSLFTAT
                     GNQHWKSIVLKLTNNCNQAVDFQNSTVSFQTTAALNTSFWGDFSPLSYPDNALNISSQ
                     PQSDGNYLATLNLHFPSYPGANSKLPVGSSISIKYGATTDSHIEGTANVYLSTTVESG
                     SIQLINAATKPTNVSQGYALVHVTMNGQSISDVQLPWQTSKTLSGLAAGNYAISAETV
                     TDSNGNLYLGQANPSTVNVIASQITSSTINYARVQETGKIKIHVQNLPGELSNYNENP
                     TVLLTQSQSGNSRPQPVVWGNTTTVAELKEGSSYQFSTPAIQYNDYNCWPTFTPSSLV
                     ASATNVPTTNLTYQCIQVVKDTVTLSVSGAPASLSSLKIILTPNDGSQTVEQTVDLAN
                     GVGSSAISLTDGVIYTLSSEGVPGYTVQFSPQPLTATANATVNITLSQVTSAKGRIIG
                     YVPGWKTPPTAQELASAGYTHVMIAFGVFSTNTPGVIVPAFETVTKEYIQSLHQAGIK
                     VILSLGGALTSIPNTTVDFHQVLVASSSPEAFKQTFINSLKGLISQYDFDGFDIDIEH
                     GINAGGSFSQPQGDIAVLASIINTMYGQNPSLLITLTPQVANIAATSGFDQTWGNYAS
                     LTMQTHQSLAWVGIQLYNTGCAFGIDQVCYGPTPTDTPDFSVAMATDLLENWPATVNG
                     RPTGFQPYISYLRPSQIVIGYPSPNASGGSDGSPVTPTTTIKRAIQCLKTAIASNTSC
                     GAYVPPRAYGNIGGVFNWEVTYDKNNQFKFAKELKNCVINGVCG"
BASE COUNT         3384 a         2013 c         2014 g         3288 t
ORIGIN      
        1 ttttctaaaa caattccatt aaccagccag gaagcctttg aaatattatg tacgagagat
       61 tatcttgaaa aaatttcaaa gattatcttt aattttcaac aattgtttaa tgttaaaaga
      121 agtactcttc tctctcatca caagctcaac cccaaaataa gtaacaatcg agaattcctt
      181 caagatctgg aagctcgtta tgatcgcctc aatcatgcag tacaaaataa tgaaccttac
      241 ccttttttat acggcgatgt atgtttattg aaagaatacc tccaagtcat tttaggctac
      301 taccaggagc aacttaaaga agagcagccg gtagcgaaaa ataatttaag aaggatcaga
      361 ggaagccata aatttccaac tctaatgtct gatatctcaa aaggggagca tcctgagctt
      421 ggcaaagagg attcagaaat tttaatcaag tacaccatta atttctgtgc agaaagcact
      481 atgtggaatg atgtaaaaac aattagtgac cttgtaataa aaccattttt atttgatcac
      541 aaagacgaac aggatttttc ttattgcaat ccataagctg gagaatagat gagagggcag
      601 tggactcaat ttgacaacat ctctatatca gatactgccc cataccctcc aagccctacc
      661 ttagaaagag tcttgatcgc ttagctagca gtattttatt tcactcgaat gaaccctgaa
      721 aatcagatcc aaaattaaaa ctattgccct ataattaaaa aagattactt ctcttattaa
      781 aaatggacca gattaagaag agacccaatt catgaaacgg atagtattta tactgttgtt
      841 tataagcaat gttctagttc atagtgaatc actcaaggtg ggtgtcttac aatttgcgcc
      901 tcctttctca tctatatcag acaaagcgaa tcactattat ggtttcgttg tcgccctcat
      961 gaatataata tgcaaccgtt tgcaagaaaa atgcgaatac atccctattc ctaaagaagg
     1021 cgagttaaaa ggcctggata aaggtgttta tcatattgtt tttagctcaa cccctatcac
     1081 gacatcatta cctgccaact atcggtacag cttgccctac ttacccagtg acggtcaatt
     1141 tctgacatta aaaaacaact cgattgatac cttgaacgat atcaatttta aaaaagttgg
     1201 tttctttaag attaattttc agcaatctcc tcttttaaaa aaatacgaaa ccaccaatac
     1261 gcttatagag tacacagatc cggcagaact agttaatgca ctaacaacca aaaaaattga
     1321 tgcgatttta atcaattatc aagcatcacg attcatagta aataatctca accccactga
     1381 agacgatcaa ttaaaattga ttggcgataa aataccgata ggaagcggat atgcgatcat
     1441 agcccttaaa aaccaatcgg cattgattga taaaataaat cacattttgc tggaaatgga
     1501 aaaagatggt tcctacctcc gtatctacaa cgaatatttt ggtgcaaacc cggcatataa
     1561 tcttcatcat tagttgatta gtatgaaaat cgccgcatat gatgcgtgtt tagaatcccg
     1621 agtaaagtac gaaaatcaag ctcgtggatt aagttatttg aattcagatt gacaacagga
     1681 attttgccta tgacttcaga atattgaatt gaattgctgc actagccgtt tcaatatcat
     1741 ctcgagtttt gtctactatt tatagcaaca cactaccgct catagataat catcctgaaa
     1801 aaatagcgac aagaatgaga aattttacaa aaatttatca atgggttatc gaataggcaa
     1861 gcgtgaaact ttgaccgaac acgggttatg tttagaatgc aggattgccg gaaacttaag
     1921 gttttttgaa agtcctgttc cattcaagat tgcttgactt tttccaaata attaactgtc
     1981 gcccttttac caacgcttct cccaagccaa tatgattcca tttcataata tcctgagctg
     2041 tgactttata aagtcgctca agagtattat acgtttcatt ggcttggaca atatgtatga
     2101 ccctgtatac tttacctttt gttggtgtct ggcttaagaa ttttgacttt gaaatttcgt
     2161 ggaaaggcaa tgctggagtg cttttggcgc ttggaattaa caagatctga ttgggtctta
     2221 tcgagtgatc tgacagacga tttaattttt ttattaatga aacagtcgta tgatattttc
     2281 tggcaatact ttgtaaacta tcacttggaa ggacgagatg ttttctccag ctcactcgtt
     2341 tatcaacagg tgaatgagat aaattgaaat aaaattgagt cacttttgct atagggataa
     2401 gcaaagtgaa aggtttatag ggtgcggtag cccatctatt ataacccgga tttaatttgg
     2461 ttaattcact aaccgaaatt cctgctaaac tagccgcatg attcaagtca atttgactgc
     2521 caatattcac ctcttcaaaa taaggtcgaa atggcattgg aggaagattt attttataag
     2581 cccgagggtt gctcatgatt tcagccagag ctaaaaatcg aggaacataa atttgagttt
     2641 ctttaggtac atccagattc caaaaactga cactacctcc gttagtgtta tttgctttta
     2701 cagctctgcc tatggctcct tcgccagcat cgtaagctgc gatggaaagt atccagcttc
     2761 cgttaaacac tttatttaaa tgcatcaggt gagtcagggc agcatcagtt gaatgattaa
     2821 tgcttcgtct tccatcaaac caccaatcct gaattaaacc taattgattc cccgtcaccg
     2881 gcattatttg ccataaacct gcagcaccaa cttttgagta agcaaaagga tcgtaagcgc
     2941 tttctatcat agggagtaat gccaactccc ccggtaaatt tcttctttgt atttctcgag
     3001 ttacatgata aagataaggc tcagcctgtt tacaggcctt agtcacaaac cctggatgat
     3061 ccagtagcca gcgaatttga tcttgaacct ctgggcgata ggcttcatga ttcatcttaa
     3121 attcactacg caaaacatcc catacatcag gtgtttcaaa tgatttagca tgagtactga
     3181 agataaaaaa aataaggcaa ctgaatagca gctgtatttt tttatcgatg aatgtcttaa
     3241 tcaaattact attcctgctc tataaagctt ctctggagcc cgtttataat gtattgtttc
     3301 tcttcgcctg aaccggccct gagtgcgtct tataaaatat actcctctta gtatcttgta
     3361 taaagcactt cctgcaatat aaggccatga taatttaaca ccctgtctat ttaacgtttt
     3421 tttattaata ttgctctaac atttgtttca atatatgctg atggttcctc cagcaattat
     3481 ttatgacaaa ctgattttta tcagagcggt tagggtttat tttcatatgg aaatgggatg
     3541 gctggttgtg tgtatcaagc atcaaggaat gataagaatt aatatattca ggagattgga
     3601 aattatgggc actttttttg aatggtttcc cagagaagta ggtttagata agaacccgga
     3661 tgcgtttagg acagattgct atcaatacta ttggggagag cctcctcgat ggggcggtac
     3721 cgttgacgca tgtgaagttg aaactgattt atacgctatg gtatataatt ccataggact
     3781 tttgagctac ttgatacctt tggtgggtgg atatttagca ttacattttg cacgaaatgc
     3841 aaactccaaa gaattttccg aagataatcc agaacatggc cttgagcagt ctgttcttag
     3901 cccttaacta ggctgaatta tttctaaaaa tcaataaatt aaaaatccag ataaaaagat
     3961 acgcttttaa atcgaatccc gttaaaagat tttcatacat caggaatttt gcctaagatg
     4021 ccggggcatt gatccggcat cagatggctt gatgagataa gataagtgtt tcagatgaac
     4081 aggaaaactc ctgttcaaat atgagttttt aaattgtatt cacattatcg gtttttatcg
     4141 atagcaaatc tttcaattca ttaccaatat gctcatcttc taacaacggc ttcatggtag
     4201 caccaagaac tccagtagtt agtatatctc tatccgtatc attgagtggc tcagcaccat
     4261 ccacctcaag attttggaac aatttggtga tagcgtttag tttgtccgtt ttactgtaac
     4321 ctgccgcccc aaaaaaactg aacagtgtcc cagccattcc ataatagtgt tcatatgtcc
     4381 tttcttgagt agaatcgata taaggtttca acgcttcttt taatcgtgca atggctacag
     4441 accttgcgtc gggttcacta acatgctctt gatgagtgac ggtgacagga acaacagtct
     4501 cttcattaat accgttatcg taatcaacgc tttcagaatc cgaattggat tcatcaaggg
     4561 tttcctcagt ttcagcagcc aattggatct cgagcaaacg tacctttttt tgcaggtttt
     4621 ctattttttc aggtaaagtc tttgtagcaa gtatgattaa actcacaatg ggatgctcag
     4681 ggtcaataaa aacccgcaaa ttatctttgt tttgtattaa ttctgaattt tcaaattgag
     4741 ataatgctct tcttttaacc tctattgact gactcaacag ggcctgatca agaatgaggt
     4801 ccaattcatt cgcttctttt tccaatgctc ttagtttaga cataactatt cccaatttag
     4861 tgataatttt tttaaacagt gattaattat aaaacaaaaa cattaagata atcttaactt
     4921 atgaaaaaag ggctatttcc cgaatgcctc aaactatctg ctgttgctct cttgcaacaa
     4981 cagatagttt gagatgctta gacttactca cctcagccaa atacctccac ggtgaataat
     5041 tacactgaat ttttagaatc attcaccaaa tttataagct gttgtaactc acatactgag
     5101 ccaccgcaag caggaataag tactggatta ccattatagg taatttttac tgtgtaataa
     5161 tttgcaccat tatcatagag agaaaaatta acatttgagg cataaggtgg ggatttttcc
     5221 aaaggagcac ctaaaaaact taatacactg gctattgtag tatcatgagc agatagcaaa
     5281 acatatttca gtttagattt tttcatgctc ccggaattca gataatcaac tatgttagtc
     5341 atcagctttg aactataaac gtttgctatt tgttgaggtt tttcttgtgc cataaaagcc
     5401 cactcagcag aattgataat tgtttcaata tcgttagaag ctaatccttc cggcatagga
     5461 gcattatgga tttgatgaat atataaggta tgaccaactg tttccagatc ctccagatta
     5521 tcaatattaa taccagtcag acggctccat aatgggtatt tgtctttcaa ttcattattt
     5581 ttttgttgcc actccctggt agaaaaaaca tattgctcca ttaacttttc ccgttcctta
     5641 cgatctacct gctgaatgat aacctcgtcg tatttagacg gtgcgctaaa tacgggaata
     5701 ggctgaaaag catgaggtaa agcggaagtt cctgcaggaa ttgttggtcc agttccaggt
     5761 ggatatagcc ccattaacaa tgactgagcg ctcatcagag tacgtgcata atccgtagag
     5821 cggacataga tggttccgta ttcatagtgt tcaggtaaca aatgcgactc ttcaatatat
     5881 tttttgcgaa atgccacacc cattttgtat tcttgttgca taccttctgc cgtaagctgg
     5941 ccaagcccct cttgccattg gtaatttaca gtaggcaaag caacaatcgg cgttctgtcg
     6001 ccatgacgaa taatgtccac agcaaaaata agtttgtctt gtgctaataa aatggagggt
     6061 acgctaatta atagtgcgaa actcaggcaa atcttgttga ccatgtattc atactccttg
     6121 agttaaagta aacatgaggc ttcgtaaaaa attcttgttg tattttgcca ccgttggtta
     6181 atttcggttt cgcactatga cgaagacaca aacaaaaatc aagacatcgg cttttgtatt
     6241 ccttgattca tttttaacct agaggaacaa tctattttat acaggaagta aagaaattat
     6301 cctgaaatcg actagaatta agatatagct catttggtgg gtgagaaaac ccttgccaca
     6361 ttggagttgc aatgagccgt tatgtatgca agtggaaatt gatttatacg tttttcattt
     6421 ttattgtctt taataattgc ccacttgcct ttgcgagccc ttccttaacc aagcaattac
     6481 ataccatatt aaaagacaat ctcgcagact cattaacccc tggagcagta ttactgatat
     6541 caagccctga tttgggcaca atttccgtcg cggcagggtt ggcagataag gaaaatgata
     6601 tttcattgaa tattaccgac agttttcgtc ttgcaagcat gagtaaaaca tttttggctg
     6661 tgactgtctt aaaactggta gcagataaac agttaaatct tgatgatcat attgcagact
     6721 tattgccaga tagtatagat attgaacgca taccgaatgg agatgaggta acagtgcgac
     6781 aattactgca aatgcgtagc ggcattccta attatacgga ttatgattct tacagtgatc
     6841 tcattgatac tatggcagac aaggaatgga caccagaaga atgcattaaa ctagtctacg
     6901 atcaaaaacc tgactttaag ccaggaacag cttatgagta cagcaatact aattatttgt
     6961 tgctgcaagt aattatcgaa aacataaccg gagaatccta tgccgaatca gttaagaaat
     7021 acatcctgaa acctcttcgt ttaaaaaata catatataga aaagcaagaa tttgatgagg
     7081 accatcatta tttatcaaca catggctata ccttggaaga tggaaaagtg gttgacgtaa
     7141 cagaccatga tgatggtttt ggcttggctg atggtggaat aattagtact gctgaagata
     7201 tcaatatttt tgtccaggca ttgttaaaag ataaaatact tttatctcca aactatttaa
     7261 caatgatgct tacttttcca gatgattacg gttttggaat aggacgcgaa gaaattaatg
     7321 gggaaatagc ctggtcccat aatggagcaa gctcaggata ccaaggccag tactactatt
     7381 ttccagagag gcaattgact gttgttattt taaccaacta ttttgacagt gatatcatcg
     7441 aagacttagt atctcagact ctcagtgcga ttgacaattc tgaataactg cttaactgct
     7501 ctcatctaat ggaagtccgc aaaatagatt atatgttata ctaagtgaaa attatccggg
     7561 cttttatggg ggcaaaatat gaccatttac aaatttgaaa tctcaactat gcaaaacggc
     7621 aatatacaga tatccgcaaa acacaagtca acaggagaga tacacggcta tgttcaaatt
     7681 gcctcatcag ccaggttaga aagcagaaat ttgctagaaa tagaccagct ttatgttgac
     7741 aataatgcca gaggacatgg cttggggagt gaacttgcct caagagccat taaatatgct
     7801 cttaaaatgg ataaaggcat tcatatgatg attcaaccag gctcggatgc gttttggaaa
     7861 aaatatttca attctcgttt tgatggaaaa cgtattacct tatttggtgg agaaacttta
     7921 aatactaatt tagctgatgg aaaagtattt gaaatttcta aagaaagttt ggttgccaat
     7981 tacaaatttc ctcaagatag acacctgcgt gatcgcgata tggaaaaaat ctgcgctaag
     8041 ctctctcatt aagcggatga tttacaaaat taaaatatat tggatcaaac agataatata
     8101 tgataaacaa tatttcaaga tttaagtgtc aggctcaaag agcctgacct cgtcaaattg
     8161 attacccaca aacgccatta ataacacaat ttttcaattc ttttgcaaat ttgaattgat
     8221 tgtttttatc ataagttact tcccaattaa atacaccacc gatattccca taagctcttg
     8281 gcggaacata agcaccacaa ctggtattac tggcaattgc tgtcttaagg cactgaatag
     8341 cccgtttgat tgtggttgtc ggagtaaccg gtgaaccgtc actgccacca ctagcatttg
     8401 gagagggata accaatgaca atttgggaag gtcttaaata gctaatataa ggttgaaatc
     8461 ctgtaggacg tccattgacc gttgctggcc aattctccaa taaatcggta gccatagcta
     8521 ctgaaaaatc aggggtatca gttggtgtag gaccatagca tacttgatca attccgaaag
     8581 cacatcctgt attgtaaagc tggataccta cccacgctaa agactgatgg gtttgcatag
     8641 ttaaagaggc ataattcccc caggtttggt cgaaaccgct tgttgcagca atattagcca
     8701 cttgaggtgt cagagtaatt aacagagagg gattttggcc gtacatcgta ttaataatgc
     8761 ttgctaagac agcaatgtca ccttgtggtt gagaaaagga accgccagcg ttaataccgt
     8821 gctcaatatc tatatcaaac ccatcaaaat catattgaga aattaacccc tttaaagagt
     8881 tgataaatgt ttgtttaaat gcctctggtg aagaagaagc cactaaaacc tggtgaaagt
     8941 ctactgttgt attggggata ctggttaacg caccacctaa agaaagaata actttaatcc
     9001 cggcttgatg gagagactga atatattctt tggttactgt ttcaaaagca ggaacaataa
     9061 cacccggagt attggtgcta aatacgccga aagcaatcat gacgtgagta taacccgcgc
     9121 tagccaactc ttgagcggtg ggtggagttt tccatcccgg tacgtaacca ataatacgcc
     9181 cttttgcaga ggttacctga gataaagtaa tgtttactgt tgcatttgct gttgctgtta
     9241 aaggttgggg agaaaattgt actgtatagc cgggaacacc ttcacttgaa agtgtataaa
     9301 taaccccgtc agttaaagaa atggcagatg aaccaacacc attggccaaa tctactgtct
     9361 gttctactgt ttgtgaacca tcgttgggag ttaaaataat tttcaatgag gacagagaag
     9421 ctggagcacc acttacacta agtgtcacag tatctttaac aacctggata cattgataag
     9481 tcaaattggt agttggaaca tttgtagcac ttgccaccaa ggaagacggt gtgaaagtgg
     9541 gccagcaatt gtaatcgtta tattgaattg caggagttga gaattgataa ctactcccct
     9601 ctttcaactc agcaacagtt gttgtattcc cccaaacgac aggctgtggt cgtgaatttc
     9661 cggattgact ctgggtaagc agaacggttg gattttcatt gtagttactt aattcgccag
     9721 ggaggttctg cacatggatc ttgatcttgc ctgtctcttg tacccgagca tagttaatag
     9781 tcgagctcgt tatttggctt gcaatcacat tcaccgtgct gggattagcc tgtcccagat
     9841 aaagattacc attactatca gtaaccgttt cagcagaaat tgcataattg cctgcagcaa
     9901 gaccggaaag cgtctttgag gtttgccaag gtaattgtac atcactgatt gattgaccat
     9961 tcatggtgac atgcactagc gcataaccct gtgacacatt tgttggtttt gtggctgcat
    10021 taattaattg gatgcttcct gattcaaccg ttgtacttaa ataaacatta gctgttcctt
    10081 cgatatgact atcagtggtt gcgccatatt taattgaaat agaactcccg acaggtaact
    10141 tgctattagc accaggatag ctgggaaaat gcagatttaa agtggccaga tagtttccat
    10201 cagattgagg ttgagaagaa atatttaaag cattatccgg gtaagataaa ggagaaaaat
    10261 ccccccaaaa agaggtattt aaagccgctg ttgtttgaaa ggagacggtt gaattttgaa
    10321 aatcgactgc ctgattgcaa ttattggtta actttaatac gattgatttc cagtgctgat
    10381 ttcccgttgc cgtaaaaaga gagtttacac aggttgtagg ttgggcaatt gtgggtttta
    10441 aagaggtatt ggtattggca ctgacacttg tcataattgc agttaaagca cccagcagta
    10501 ataatgaata tcgcatcaaa ccctcctcat ttatgattgg aaaaaaatta atctatcatc
    10561 attttttctt atttaacaat aagctataag aaaattgtca atttctttac aattagtttt
    10621 ttagcttaaa tgaaatagct aaaccactta gcaatcagga tggtcaattc acagatttac
    10681 tttgccatgt cacaaaatt
//
LOCUS       DASLOE010000103        10683 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.104, whole genome shotgun sequence.
ACCESSION   DASLOE010000103 DASLOE010000000
VERSION     DASLOE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10683
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.104"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            62..379
                     /locus_tag="U6309_002016"
     CDS             62..379
                     /locus_tag="U6309_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEN5505454.1"
                     /translation="MTRKLRIEEKLISALSPFFLSVEDESMNHHVPQGAETHFKIIAV
                     SNHFNDLTRINRHKFVNQLLKEEFTLGLHALSMHLYTSDEWMTQQQSVLKSPACRDGY
                     KNK"
     gene            454..1875
                     /locus_tag="U6309_002017"
     CDS             454..1875
                     /locus_tag="U6309_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN5505455.1"
                     /translation="MKSWSSEKISVLALVLLITGAIDSIRNLPGTALFGSTLIFFFIF
                     SAIIFLIPVALVAAELSSTWSDEEGGIYSWVKHAFGENIAFFAIWLQWINTLVWYPTI
                     LSFIAGTISYLINPELAQNKYYLISVILIIFWSLTLIGLSGLRASATFAGFCAIVGMI
                     VPMGFIILLAFIWLIHGHPIAIDLSLSNLLPQWKNTQSWVSLTAIMTSFLGMELAAVH
                     VRNVNNPQKNFPKAMFFSVILILFTMILGSLAIAFILPKDKISLVDGVMRAFSNFLQV
                     YDLNWLMPVLILLLLLGTLGSMINWIISPAKGLLMAAKHDFLPGSLCHVNKHGMPAKI
                     LILQAILVTVLCSGFLLFPTVNAIYWLFTALSTELYMMMYVMMFIAAWHLKSKFSDME
                     RPFAIPGGKPGYYLTCLLGLIGCTVTLWVGFIPPTESINIGGTAHYRMVFSLGILLML
                     LPAALIYLRKRHITKSKALNAPV"
     gene            complement(1973..3403)
                     /locus_tag="U6309_002018"
     CDS             complement(1973..3403)
                     /locus_tag="U6309_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEN5505456.1"
                     /translation="MKIFKFSSWLLLFVFTLDANAYYTSQGSIYDKSGNRVTINGVSW
                     SGFQDTNVFQGLQSNPFYNITMPQSKPRNGLMDLLVHPWSMADSGVNSSTAVEFKTVR
                     LPIQPGVLYDDSGEVDLNKWLSDKTQPEAGNGLFCKTWQSNGQSCEKAVSPKQAFWTV
                     LAEMKKHNINVMIDFHHRYGYGDGMRDGTVYSMAQYEKDLVLLAQEIKQRGLDNVIGI
                     DVFNEPYQLSWFKARNGQVSWITVIATAAKAVHQVNPDLLLFVEGPGGGNDDMDNPTI
                     CVPKANIKEDSGYAHWRDPGQCGNDQEVVAFKGNWGEDFKPLLDKNLAKQNIAQFDVQ
                     KFSNELQLAVPGIDTETLGWLLGDDNMGNNGHLVFSPHVYPKEVAGWETAPGDASNLR
                     FDWSWGFLYKAGYPIVLGEASWKSFEGKNFFTQALMPYLTQSGIGSNNLYFWAIGYLG
                     DTVSAINPNTGELNLDVQQTLKPYFN"
     gene            complement(3651..4403)
                     /gene="kdsB"
                     /locus_tag="U6309_002019"
     CDS             complement(3651..4403)
                     /gene="kdsB"
                     /locus_tag="U6309_002019"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEN5505457.1"
                     /translation="MSHNFHVIIPARYHSSRFPGKLLQEINGITVIERVYRQALLAEP
                     KSVIIATDHDEIADRAIQFGAEVVITSHTHQTGTDRIAEVVAKGSFAPDDVIVNVQGD
                     EPFIRPKLIQQVACSLTKTKAPVSTLCWPISSLEILNNPNVVKVVCTRDNHALYFSRS
                     AIPFHRDDKNAYSNTFRHIGLYAYRAAFLLEFVSWPLCTLEQIECLEQLRILWSGFSI
                     RVDEACEEPLQDINTKEDLILAQQYFLDTFNV"
     gene            complement(4400..4579)
                     /locus_tag="U6309_002020"
     CDS             complement(4400..4579)
                     /locus_tag="U6309_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019231979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEN5505458.1"
                     /translation="MDKKLLEILVCPLCKGKLLFKKQELICKFDRLAFPVRDDIPVML
                     EQEARLIPLEEKDKL"
     gene            complement(4572..5243)
                     /gene="tsaB"
                     /locus_tag="U6309_002021"
     CDS             complement(4572..5243)
                     /gene="tsaB"
                     /locus_tag="U6309_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEN5505459.1"
                     /translation="MMKLLAIDTSTELASVAILIGDEIISREQDSQRIHAQLILPMID
                     ELIAQAGLGLNQLDGIIFGCGPGSFTGLRIACSIAKGLAYANDLPLVPVSSLAAIAWT
                     AREIKEDFNQPVLSVLDARMHEMYWSCFLEQQFLAQDRINAVKDILLPANQSFILAGV
                     GIDLYWMDFPEQIKSQTSEVLTVFPTASAMIRLAQKANIKAVSVAQAQPVYVRNQVTQ
                     GDSRG"
     gene            complement(5221..7179)
                     /locus_tag="U6309_002022"
     CDS             complement(5221..7179)
                     /locus_tag="U6309_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEN5505460.1"
                     /translation="MEEITTFAETCRRTLSEKGRLSTAIPGFVARAPQADLAVAIANA
                     IVQRSILVAEAGTGTGKTFAYLLPCLLSGKKALISTATKTLQDQLFQKDLPTLIRALG
                     LSVRIQNLKGRSNYICQYRVEKHAEEGQFQSPQCAHEILHVRDKLSQMQEGVRSELPE
                     INEDSSVWHYVTSTTDNCLGAECPNHETCFLVKARKRAMEADIVVINHHLFFADSQLK
                     KEGFSELLPGVDVVVFDEAHQLADIASNFNGERLGTRQFKDLVDDMLNEWPILDLANQ
                     PLKSISHKADKLVDQLLNALPTREERISWDLVKRNKEFMEAWNTWLSLKDELIQCMES
                     KDTADIPGLKRCKERLLDLETVLLSFTEENNHKIRWLERFKHTLVFHATPYDVAESFS
                     QLLKDQSCAYIFTSATLTMASVFDSFCKPLGLDKVQTLLLSSPFDYQQQALLYLPRGI
                     PDPKDAHYYEALLEKALPVIEACGGRCFFLFTSHKAVKLVAQMMSNTLKYPLLIQGDE
                     AKPILLSRFRQLGNAVLLGTSTFWEGVDVKGEALSCVIIDKLPFANPVDPVIRGRMAY
                     LKKKGLSGFDELSLPNAVIALKQGVGRLIRDITDKGILMIGDPRLTGREYGRTILASL
                     PGLPKTRDEEKVLHFIKELALNDETVSN"
     gene            complement(7167..7502)
                     /locus_tag="U6309_002023"
     CDS             complement(7167..7502)
                     /locus_tag="U6309_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEN5505461.1"
                     /translation="MTFVVTESCIRCKYTDCVEVCPVDCFYEGPNFLVIHPDECIDCA
                     LCEPECPVNAIVSEDDLTEEQQQFKELNAELSKTWPNITAKKDAPSDAKDWEEVKDKL
                     QYLQKEWKK"
     gene            7785..10574
                     /locus_tag="U6309_002024"
     CDS             7785..10574
                     /locus_tag="U6309_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1924 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505462.1"
                     /translation="MPLNLPPKSSKDIMPLVIAYNNAPEDNKIQKLFYLQKINYLLNK
                     TQLNDDLFDWINDAEEGGWLNELAKFSINPNASFFLKGMQFAKAITEEIKNKPEINSS
                     EANIYQLMQERDQLLKEVEFEKCATRYAEINFLLNELALNDKKTKEIVERQTEILRLV
                     APKIKAIKGESTDNLQVIPSYKTKELGNHVNNFNFKFTMSGWEAPFVFRVEDRHELGK
                     EQELHSYGVSKYFIEDYSVFMMRFKAEDGSTVYKPVILSQFANQNNLEEIAKQLKDGS
                     PKNIAPRIGYYFVQLTDFCLKLIETHNYHPDIKLNNFLVHNNRVLVSDRKTFTTNDNP
                     LASEILTSPLFAPDEFLKCLLFNKEGDPVGYNRNALWKRMNMPQFMAYQLGMALKQFL
                     ILTQLDELPDNFRNPDHSAISHFKTPSRQIINLSLLVQELTRLDPDKRMTIKQFQTLL
                     NFKNLPPDAFYQKVEEVFPSSQLGIAEDIEALNKVLNSDLKGEALLKQANPVFTKLSK
                     YDPKETRLTRLAEKLAIRCFNNVSKPYFQKLSGLIESALEKDVNEIRQLLNPQSNSEG
                     LLQKASPIFMKLFERQPEVPQLTDLAKQLAAQCFDESSKNYFTRQLPLLVKEELLNQD
                     WEQAPWYRKALHWLTFGYFRVDNVTEISSLKIPEHTKGKEFQIYFSQLLFLPSEEIKN
                     MFTSKDSQTAEFLLGYIEKEREKRETETPSVQIPVDLKGEEFQIHFPQLEFLPSKDFE
                     SIGEKEGEHLECFIFANLQEILSHNNSDSSERNSTSEPTDSESEIDLNVGNAAGTIII
                     ADEPNPTEAKPQKKDQDGAKQNNPAKASSPEGKISTPELDEHPKKQITDDVPKPKTKR
                     SVHFFDEAKKPKEKENASPAVDETPPAKRKNVCRIDSVRSTLFRGDGSHRQKIKEQRP
                     RLSEIAWEPPKPNLQ"
BASE COUNT         3353 a         2268 c         1917 g         3145 t
ORIGIN      
        1 gtgacaacta tgcttcaaat gtatatactc cttattttgc aaaaacagag tcgagcaaat
       61 catgacgcgt aaactccgca tagaagaaaa actaatctcc gcattatccc ccttcttttt
      121 aagtgttgaa gacgagtcca tgaaccatca cgttcctcaa ggcgctgaaa cacattttaa
      181 aataatcgcc gtttcaaatc attttaatga tctgactcgt attaatcgcc ataaattcgt
      241 taatcagctt cttaaagaag aatttactct ggggctgcat gcattaagca tgcatttata
      301 tacttctgat gaatggatga ctcaacagca atcggtactc aaatcccctg cttgcagaga
      361 tggctataaa aacaaataat tttaatgaaa aaagttcacc tccaagcttg atttatttta
      421 taatgtctat ctttttatca aatagaaaaa tcaatgaaat catggtcctc tgaaaaaata
      481 tccgttcttg ctttagtgct gctcattacc ggagcaattg acagcattag aaatttacca
      541 ggtacagccc tctttggttc tactttgatt tttttcttta ttttttcagc gattatcttt
      601 ttgataccgg ttgctttggt cgccgctgaa ctttcctcaa cctggtcaga cgaggaaggt
      661 ggcatttaca gttgggtcaa gcatgccttt ggtgaaaaca tcgccttttt cgccatttgg
      721 ctgcaatgga taaatactct ggtatggtat ccgactattt tgtcttttat agcaggcaca
      781 atctcttatt taattaatcc agaacttgca caaaacaaat actatcttat cagcgttatt
      841 ctgattattt tctggtcatt aactttgata ggcttatcag gactgcgtgc ttctgccacc
      901 tttgctggct tctgtgcaat agttggaatg atagtgccta tgggatttat catccttctg
      961 gcttttattt ggttaattca tggccaccct attgccattg atctgagcct ctccaatttg
     1021 ctaccgcaat ggaaaaacac acaatcctgg gtttctctaa cagcgattat gacttctttt
     1081 ctcggtatgg agcttgctgc agttcacgtg cgtaatgtca ataacccgca gaagaatttt
     1141 cccaaagcga tgtttttttc ggtcattctc atcctgttca ccatgatttt aggctcactg
     1201 gccattgcat tcattctgcc taaagacaaa atcagtttgg tggatggggt tatgagagca
     1261 tttagtaatt tcttgcaagt ttatgatttg aattggctaa tgcctgtatt aatactcttg
     1321 ttattgctgg gtaccttagg cagcatgatc aactggatta tttctccagc caagggcctg
     1381 ctgatggcag ccaaacatga ttttctgcct ggttccctat gtcatgtgaa taaacatggc
     1441 atgccagcaa aaattttgat actgcaagcc attctggtga cagtattatg tagcggcttt
     1501 ctcttattcc ctacagtgaa tgctatttat tggttgttta ctgctcttag tacagagctg
     1561 tatatgatga tgtatgtgat gatgtttatt gcagcctggc atttgaagag taaattctca
     1621 gacatggaaa gaccttttgc tattccagga ggcaaacccg gttattacct gacatgcctg
     1681 ttaggattaa taggttgtac tgtcacttta tgggtaggtt ttattcctcc aacggaaagc
     1741 attaatatcg gaggaacagc ccattaccgt atggtctttt cattaggcat cctcttaatg
     1801 ctgctacctg ctgccttgat ctatttgcgt aaacgccaca ttaccaaatc caaagcgctt
     1861 aatgcacctg tttaagtaac cgataggatg attataacct taacaataat taggaaccgt
     1921 atatattatc aggagtggcc ataaataaga gccactcctg aaacgagatg tattaattaa
     1981 aataaggctt caatgtttgt tgtacatcga gatttaattc gccagtattc ggattgatag
     2041 cactaaccgt atcacctaaa taccctatcg cccagaaata taaattattg gacccaatgc
     2101 cgctttgggt aagataaggc atcaatgctt gggtaaaaaa gttttttcct tcaaaagatt
     2161 tccaggaggc ttcccctaaa acaataggat acccagcttt gtacaggaaa ccccaactcc
     2221 aatcgaaacg cagattactt gcatcaccag gggctgtttc ccaaccagcc acttcttttg
     2281 ggtaaacatg aggtgagaaa accagatggc cattatttcc catattatca tcacccaaca
     2341 gccaaccgag tgtttctgtg tcaatacccg gaacagccag ctgcaattca ttactaaatt
     2401 tttgtacatc aaattgcgct atattttgtt tagccaaatt cttatccaga aggggtttaa
     2461 aatcttcccc ccagtttcct ttgaatgcta caacctcctg atcattgcca cactgccctg
     2521 gatcgcgcca gtgtgcatag cctgaatcct ctttaatatt ggcttttggc acacaaattg
     2581 ttggattatc catatcatca tttccgcctc caggtccttc aacaaacagt aacaaatcag
     2641 gattgacctg atgcactgcc ttggcagcag tagctatcac tgtaatccac gaaacctgac
     2701 catttctggc tttaaaccag ctcaattgat agggttcatt aaaaacatca atgccaatga
     2761 cattgtccaa accacgttgt ttgatttcct gagcaagcag aactaaatcc ttctcatatt
     2821 gagccatgct ataaacagtg ccatccctca ttccatcgcc ataaccataa cgatggtgaa
     2881 aatcaatcat cacattgata ttatgttttt tcatttcagc aagtaccgtc cagaaagcct
     2941 gcttcggact cactgctttt tcacacgatt ggccattgga ctgccaggtc ttgcaaaaaa
     3001 gaccattacc agcttcaggt tgtgttttgt cagaaagcca tttgttcaga tcgacttccc
     3061 cactgtcatc ataaagaaca cctggctgta taggcaaacg tacggtcttg aactctaccg
     3121 ctgtgctgct gtttacacca gagtctgcca tagaccaggg gtgaaccaat aaatccatta
     3181 atccattccg aggtttggat tgaggcatgg tgatattata gaaaggattg ctctgcaatc
     3241 cctgaaaaac attggtgtcc tgaaatcctg accaggatac tccattgatg gtcaccctat
     3301 tcccactttt gtcatagata ctgccttgac tggtataata ggcattcgca tccagggtga
     3361 atacgaacag caaaagccaa ctgctaaact taaatatttt catgcgcttt tcctcattaa
     3421 aataaccata aagttattgt taagttggtt aaaagctcat tatttaacca caaaactaca
     3481 atatttgaac agtgtaatca ttaatttttc aaaaatatta caaaaagatg tcatttcgat
     3541 gtgctgaaaa atagtcaaga tcgtcactcc attatgatga aaattgagtg gcgaccatat
     3601 ggagagaata aaatctttcc ctcatagaaa aaacgataaa ttacaacgag ttatacattg
     3661 aaagtatcaa gaaaatattg ctgagccaaa atcaaatctt cttttgtatt aatatcctgt
     3721 aaaggttctt cgcaggcctc atcaacccgg atagaaaaac ctgaccatag gatgcgcaat
     3781 tgctccaaac actctatttg ttccaaggta cagagcggcc agctaacgaa ttccagtaaa
     3841 aaagcagcac gataagcata taaaccgata tgcctgaagg tattggaata agcgttttta
     3901 tcatctctgt gaaaaggtat cgcgcttctt gaaaaataaa gagcgtgatt atctcgagtg
     3961 cataccactt taaccacatt agggttattt aatatttcaa ggctactaat aggccagcat
     4021 aaagtagaca caggcgcctt tgtcttcgtt aaagagcaag caacttgttg aattaatttg
     4081 ggtcggataa atggctcatc accttgcaca ttgacaatga cgtcatcagg agcaaagcta
     4141 cccttggcga caacttctgc aattcgatca gttccagttt ggtgcgtatg tgaagtgatt
     4201 acaacctccg ctccaaattg gattgcacgg tcagctatct catcatgatc agtggcaata
     4261 atcaccgact ttggttctgc cagaagtgct tgcctgtaaa ctcgttcaat cacggtaatg
     4321 ccgttaattt cttgaagtaa tttacctgga aaacgactgg agtgatatct ggctggtata
     4381 atcacatgaa aattatggct cataatttat ctttctcttc cagaggaatc aaacgcgctt
     4441 cttgttccag cataacagga atatcatcac gaacaggaaa tgccagccta tcaaatttgc
     4501 agatcagttc ctgtttttta aacagcaatt ttcctttaca taaaggacaa accaatattt
     4561 ccaataattt tttatccacg cgaatctcct tgagtaacct gattgcgcac atacactggt
     4621 tgtgcctgcg ccacagaaac tgccttgata tttgctttct gtgccaaacg tatcatagca
     4681 gacgctgtcg gaaaaacagt taatacttca ctagtttgtg atttgatctg ctctggaaaa
     4741 tccatccagt acaaatcaat cccaactccg gccaaaataa aggattgatt ggctggcagc
     4801 agaatatctt ttactgcgtt aattctgtcc tgggctaaga attgctgctc caaaaagcaa
     4861 ctccaataca tttcatgcat acgagcatcc aaaacagaca atacaggctg attaaaatcc
     4921 tcttttattt cccgagctgt ccaggcaatt gcggctaaac tactcacagg aacaaggggc
     4981 aaatcattgg cataagccaa cccttttgca atgctacacg cgattctcaa tcctgtaaaa
     5041 cttccaggac cacatccaaa aattattcca tccaattgat taagacccaa tccggcttgg
     5101 gctatcaatt catcaatcat aggtaaaatc aattgggcat ggattctttg actatcctgc
     5161 tctctgctta tgatttcgtc acctataagg atggcaacac tggccagttc agttgaggta
     5221 tcaattgcta acagtttcat catttaaagc caactcttta ataaaatgca gtactttttc
     5281 ttcatcccgg gttttcggaa gtccaggtaa acttgccaaa atggttcgac catactctct
     5341 accagtcaaa cgtggatctc ctatcattaa tataccttta tcggtgatgt cacgaattaa
     5401 tcgccctact ccctgtttta atgcaatcac ggcgtttggc aaggataact catcaaaacc
     5461 ggaaagccct tttttcttta aataagccat tctgccacga attacaggat caacggggtt
     5521 agcaaagggt aatttatcta taatcacgca agaaagtgcc tcccctttta catcaacacc
     5581 ttcccaaaaa gtagaagtgc ctaaaagcac agcattgccc aattgcctga atcgagacag
     5641 gagaatgggt tttgcttcat caccttgaat caaaagcgga tattttaaag tattgctcat
     5701 catttgagca accaatttta ctgctttatg actggtaaaa aggaaaaagc aacggccacc
     5761 gcaagcttct attactggca aagccttttc cagcaaagct tcataataat gagcatcttt
     5821 aggatcgggt attcctcgag gcaaataaag cagagcctgt tgctgataat caaatggact
     5881 ggataataac aaagtttgta ctttatccaa tcccagaggt ttacaaaaac tatcaaaaac
     5941 cgatgccata gtcagtgtag ctgatgtaaa aatataggca catgattggt cttttaataa
     6001 ttgactaaat gattcagcta catcatacgg ggtggcatga aaaacaagag tatgtttaaa
     6061 acgttctaac cagcgaattt tatgattatt ttcctcggtg aaagaaagca agacagtttc
     6121 caaatccaaa aggcgctctt tacatctttt taacccggga atatcagcag tatcctttga
     6181 ttccatgcat tgtatcaact catcctttaa agataaccag gtattccacg cctccataaa
     6241 ctccttattt cgtttaacca aatcccaact gattcgttcc tcacgagtcg gcaaagcatt
     6301 gagcaattga tccaccagtt tgtctgcttt atgacttata gactttagag gttgattggc
     6361 taaatccagg attggccact cattaagcat gtcatcgacc aagtctttga attgacgtgt
     6421 tcccaagcgc tccccattga aattagaggc gatgtctgca agctgatgag cttcgtcaaa
     6481 cactacgaca tcaacaccag gtaatagttc gctaaaacct tcttttttta actgagaatc
     6541 agcaaaaaaa agatggtgat tgataacgac aatatctgcc tccatcgctc gcttacgcgc
     6601 tttcactaaa aaacaggttt catgattcgg acattctgca ccaagacagt tatcggtagt
     6661 ggatgtgaca taatgccata ctgaggaatc ttcattaatt tcaggtaatt ccgagcgaac
     6721 gccttcttgc atttgagaca atttatctct gacatggagt atttcgtgag cgcattgcgg
     6781 gctttgaaat tgtccttcct cagcatgctt ctcaactcga tattgacaaa tataattgga
     6841 acgccccttt aaattctgga ttcgtactga taaacccaaa gctctaatca aggttggcaa
     6901 gtctttttga aaaagttgat cttgaagcgt tttggttgct gttgagataa gcgctttttt
     6961 accacttaac aaacaaggca ataaataagc aaaggtctta cctgttcctg ttcctgcctc
     7021 agctaccaaa atcgaacgct gaacaatagc attggctata gccactgcca aatcggcttg
     7081 tggagctctt gccacaaagc ctggaatagc agttgatagt cttcccttct cactgagagt
     7141 tcgccgacag gtttcagcga aagtggttat ttcttccact ctttttgcaa atattgaagt
     7201 ttgtccttta cttcttccca atccttggca tcagagggag cgtccttttt agcagtaata
     7261 ttaggccagg ttttggataa ctcggcattt aattctttga attgctgttg ttcctctgtt
     7321 aaatcgtctt ctgaaacaat agcattaact ggacattcag gttcacaaag ggcacaatca
     7381 atgcattcat cggggtgaat caccaggaaa ttaggcccct cataaaaaca atctacaggg
     7441 catacttcta cacaatcggt atacttgcat cgtatacaac tttccgttac tacaaaagtc
     7501 atagcaaacc ttaaaaatta tctttaagaa atgactcata ttaaagcata aaataccctg
     7561 ttgatgatag aggagcctga tatttttata cctctgggtc ccattcatta ttcagaacca
     7621 acctgaaatc ataaactcaa aaccaacggc atcatatgca ttcttactgc tcataaaatg
     7681 cacttgaagt ctcgctacaa atatggtaat tttgatcatt ttaaatctat tttgttcaat
     7741 attttaatgc actgtctgtg taattagata tctcggagta cttcatgcct ttgaatttac
     7801 cgcctaaatc atctaaagat ataatgcctt tggtcattgc gtataataat gcacctgaag
     7861 ataacaagat tcaaaaatta ttttatttac aaaaaatcaa ttacttactg aataaaactc
     7921 aactaaatga tgatttgttc gattggataa acgacgcgga agaaggtggc tggttaaatg
     7981 agctggctaa attttcaatt aatcccaatg catccttttt tttaaaagga atgcagtttg
     8041 ccaaagccat tactgaagaa ataaagaata agcctgaaat taacagttct gaagcaaaca
     8101 tctaccagct catgcaggag cgtgaccaac tgttaaaaga agtagaattt gaaaaatgtg
     8161 ctactcgcta tgctgaaatt aattttctcc tgaatgaatt agcccttaac gataaaaaaa
     8221 caaaagaaat tgtagaaaga caaacagaaa tccttaggct tgtcgctcca aaaataaaag
     8281 ccattaaggg agaatccaca gacaatttgc aagttatacc ttcttataaa accaaagaac
     8341 ttggcaatca tgtgaataat tttaacttca aattcacaat gagcggatgg gaggctcctt
     8401 ttgtatttcg tgtagaagac aggcatgagt taggaaagga acaagaacta cactcctatg
     8461 gagtctcaaa gtattttatt gaagattact ctgtttttat gatgagattt aaagcggaag
     8521 atggttctac tgtttacaaa ccagttatcc tcagccaatt cgccaatcaa aataatctgg
     8581 aagaaattgc caaacagtta aaagatggca gtccaaaaaa tattgcaccg cgaattgggt
     8641 attactttgt ccagttaacc gatttttgtc taaagttaat agagactcat aactatcatc
     8701 ccgacattaa gttaaataat tttctggttc acaataacag agtactagtt agcgatagaa
     8761 aaacattcac cacgaatgac aatcctttag ctagtgaaat tctcaccagt cctttatttg
     8821 ctcccgatga atttttaaaa tgtctactgt ttaacaaaga aggcgaccct gtaggctata
     8881 atcgcaatgc tctgtggaaa cgcatgaaca tgccccaatt catggcctat caactgggaa
     8941 tggctttaaa acaattcctg attttaactc aattggatga attaccagat aattttagaa
     9001 atccggatca cagcgccata tcacatttca aaacaccgtc cagacaaatc atcaatcttt
     9061 cccttctggt tcaggaatta acacgcctcg atcccgataa gcgaatgacc attaaacaat
     9121 tccagacctt gcttaacttt aaaaacttac cgcctgatgc cttttaccag aaagtggaag
     9181 aagttttccc ttcctcccaa ttagggattg cagaagatat tgaggctctt aataaagtat
     9241 tgaacagcga tttaaaaggc gaagcgcttc tgaaacaagc taatcctgtt ttcactaaac
     9301 tatcgaaata tgatcccaaa gaaacccgat taactcgtct agctgaaaaa ttggcaattc
     9361 gatgttttaa caacgtgtca aaaccttatt ttcagaaact gtctggtttg attgaatcag
     9421 ccttagaaaa agatgttaat gaaatcaggc agctattaaa tcctcaatca aatagcgagg
     9481 ggcttttaca gaaagccagt cccattttta tgaaactctt tgaaagacag cctgaggtac
     9541 cccaattaac agacttggcg aaacaattag cggctcaatg ttttgatgaa tcttcaaaaa
     9601 actattttac ccgtcaactg cctttattgg ttaaagagga gttgttaaac caggattggg
     9661 aacaagctcc ctggtataga aaagcactgc attggttaac ctttggatac ttcagagtcg
     9721 ataacgtaac agaaatatca tccttgaaaa tacccgagca cacaaaaggt aaagaatttc
     9781 agatttattt ttcgcaactc ttatttttac cctctgaaga aatcaaaaat atgtttacct
     9841 ccaaagacag ccaaacagct gaatttcttc taggttatat cgaaaaggaa agagaaaaaa
     9901 gagaaacgga aactccttca gtccaaattc ctgttgattt aaaaggtgaa gaatttcaga
     9961 tacacttccc gcagcttgaa tttttaccgt ccaaagattt tgaaagcatt ggtgaaaaag
    10021 agggtgagca tctggagtgt tttatctttg ccaatcttca agaaatacta tcgcataata
    10081 acagtgattc atcagaaaga aatagtacta gcgaacccac tgacagcgaa agtgaaatag
    10141 atttaaatgt tggcaacgca gcaggaacaa ttataattgc ggacgaacca aatcccaccg
    10201 aagcgaaacc gcagaaaaaa gaccaggatg gagcaaaaca aaataatcca gccaaggcca
    10261 gtagtcctga aggaaaaata tcaactccag agcttgatga gcatccaaaa aagcagataa
    10321 ccgatgatgt ccccaagcca aaaacaaagc gttctgtaca cttctttgat gaggctaaaa
    10381 aacccaagga gaaagaaaat gcctctcctg ctgttgacga aacgcctccc gcgaagagaa
    10441 aaaatgtctg caggatagat agcgtcaggt ccaccctttt cagaggagat ggcagccata
    10501 ggcagaaaat caaagaacaa agacccaggt tatctgaaat agcttgggag ccgccaaaac
    10561 ctaatctaca atagcaaaca gaggctttct gcagccaccc tgattgtaca gggtggctaa
    10621 cttaacaaat gtcatggtgg gtttgaaact ttattttctc tttttgttaa aaaattgcac
    10681 atg
//
LOCUS       DASLOE010000104        10676 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.105, whole genome shotgun sequence.
ACCESSION   DASLOE010000104 DASLOE010000000
VERSION     DASLOE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10676
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.105"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            128..1738
                     /locus_tag="U6309_002025"
     CDS             128..1738
                     /locus_tag="U6309_002025"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEN5505463.1"
                     /translation="MNAPLTVLMAQINPTVGAIDANTNKILDVIKNHQASHDVIIFPE
                     LTLSGYPAEDLLFRKEYHDKILINLHDIQDTTKDCYVIVGHPMMHIGDCYNGFSIFYQ
                     GEKIREYHKQKLPNYGVFDEARYFTPGKKDPCVLSIKNHKLGFCVCEDLWQKGPVDDL
                     LDSGISILISLNASPFDYRKYQLREELLRSYAKQGVSIIYVNQIGGQDDLLFDGQSLA
                     MDNQGIIRALAPAFEESLCTVNIDGNKIDGSITPYLDKEALIYKALVCGTRDYVRKNN
                     FPGVLLGLSGGIDSALTLAIAVDALGAEQVHAVLMPSRYTASISNEDALEQLSNLKVA
                     HSIIPIEPMFQSMIESLHPVFKDLPPDTTEENIQARIRGMLLMALSNKTGKMVLTTSN
                     KSETAVGYATLYGDMAGGFAVLKDVLKTQVYELVRYRNKISLVIPERVITRPPSAELK
                     PNQTDQDSLPEYGELDAIIMAYMEQNLSPSEIIAKGFKPQVVNKIIQLIKRNEYKRRQ
                     AAPGIKISNLAFGKDWRYPITNGFNQCI"
     gene            complement(1790..3238)
                     /gene="ravH"
                     /locus_tag="U6309_002026"
     CDS             complement(1790..3238)
                     /gene="ravH"
                     /locus_tag="U6309_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavH"
                     /protein_id="HEN5505464.1"
                     /translation="MDKEQGAYMPPKDGKELLDIIDNLNEFAEKLLSRIADESDEGCK
                     QLVHVLESARARYNKPIESWLKGWFYQFTRKRGQEIDMAIKGMEAFPDAYTRLQEFKL
                     LVGKGEWEHGSFNYYLFDELIKSVPGYKPLESELVHPIILKLRQKINKRIDEFMSQYL
                     ATQKLIEARKQELLQTHQSAQKFIDNVSITNDLEAAKKSAKENKGVIQFCLIFESDIW
                     KLSWVDATGKVYALEPGEELIQILIDGKIQDIVKDIDNLSALHLKQLKQECVKAREML
                     LGRVQLLINPKDPRTQAELTNDVLKENGTSATFVLRGKPNEYSLWWINTLGFAHEISL
                     ESYASMKTWLNDQSHPLKEEHIPQLKTYLLNVNTVKSIDVKEDMKEFKAQLQECLAAG
                     PKSKTPPASGATKTTNAKKLDLRLFGDVERCLRSQLSERQEEKAAIQEPGKINLNKYE
                     NIATLFGNRAKNMEQHEGQQSKPNDSLSIRSI"
     gene            3404..4042
                     /locus_tag="U6309_002027"
     CDS             3404..4042
                     /locus_tag="U6309_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEN5505465.1"
                     /translation="MRITLLTTAILATGVASAAVPVDGWYASAFGGYTYIPPNIYNFA
                     YGVFRDEVGYNNGYNAGGRVGYQSNPIRYEVEYTYLRGEARKFNLNFITQLGVTGFTS
                     ANTIMANIYYDCPDMLPAIAPFIGLGIGYASLRTELDSIGPFRPSYFTTSDSAFAYQG
                     TVGITYNFSENYAANLAYRYIATDNSSNFGKTYQAHLASVGAIYRFDQGNYK"
     gene            4052..4789
                     /locus_tag="U6309_002028"
     CDS             4052..4789
                     /locus_tag="U6309_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEN5505466.1"
                     /translation="MKKLIQLSVLSGLLLSGSTFAFNQPQGFYVGILGEISHGPSGED
                     LIFTEPNRTITANVEYSPVSGGGGAVLGYKISHYRIEGEVLYNRISTGPFTISSCTLQ
                     SPNVLTPTGVCPSAFEVNRLGYDGSSAALYGLLNLYYDFFSSVNESTYVPYIGIGAGQ
                     VRIQNRNNFVKTTPVNLPASAGFSTTMVSGAGQGIVGVGYLMDDFAWLAMDFRFLTTS
                     SLSDFNNSRYTLSTFNVGVNFTFDKST"
     gene            complement(4781..5833)
                     /locus_tag="U6309_002029"
     CDS             complement(4781..5833)
                     /locus_tag="U6309_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN5505467.1"
                     /translation="MNENHKELISIGLTVSIVIFSLYIVHKFIPSMIWAAIIVIATYP
                     LYTRWRKLFGNKHNTSAFLFTTLMGLLFLIPLSWLIGILIKESQLFINFLQHINKEGG
                     AAPEFFKNIPLVGDDLIQYWDVNIGKPGSIKEFLSNLHVTLTPTSYYIKQIGVNLAHR
                     SFQVGFTLLTLFFFYRDGDKLLLQIQHIGEYCLGDRWFRYSDRLPSALRGTVNGTIVV
                     GIGVGILMGICYALVGFPAPTLTGFITALAAMIPFVVPIVFIIVALILLSVGSLIGGI
                     IVLVWGTLVMFVADHFVKPVLIGGAIQLPFLAVLFGILGGVETLGLLGLFLGPMVMVL
                     FVTLWQEPQGHKKSSG"
     gene            complement(5833..6315)
                     /locus_tag="U6309_002030"
     CDS             complement(5833..6315)
                     /locus_tag="U6309_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEN5505468.1"
                     /translation="MNQVNLANRVWQDPVYFIAFGFGSGLMSIAPGTWGTLAAIPLYL
                     LMINAHWSIYLLWTALAFALGVWVSDRVTEDLGVHDYKGIVWDEVVGYLLTMFLAPKG
                     ISWMLIGFILFRIFDIWKPQPIRMIDQRVHGGLGIMLDDVLAAVPAWCIMQILVWSFA
                     "
     gene            complement(6405..7361)
                     /gene="thiL"
                     /locus_tag="U6309_002031"
     CDS             complement(6405..7361)
                     /gene="thiL"
                     /locus_tag="U6309_002031"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEN5505469.1"
                     /translation="MNEFTLIDHYFKSIPCKRNDVLFGIGDDAACLQVPDGMHLLVST
                     DTLVSGIHFLPQWDPYDIACKAVMVNISDMAAMGAQPCWVTLALTLPELNQSWLDSFA
                     RGIKDSLGLYNVDLIGGDTTKGPLSITLTIMGLTPQGQAIRRCGAQAGDAIIVSGELG
                     AAGLAVKLLDGHKKVNAKDKIELMNKLLHPKPRVDLCHLLRKYATSAIDISDGLGADL
                     NHICVASGVGANLFEEAIPRHPLLNQYCPEQALDLALSGGDDYELCFTVSTQQLDSFN
                     RDLQKENLICFPIGVIEEKPGLRIQSADSPSKELQPRGYSHF"
     gene            complement(7354..7797)
                     /gene="nusB"
                     /locus_tag="U6309_002032"
     CDS             complement(7354..7797)
                     /gene="nusB"
                     /locus_tag="U6309_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEN5505470.1"
                     /translation="MEKQSIRGKRRARKFALQALYQWLMSGTDLHEIEAQFRTINNMD
                     KVDGEYFCRLLYGIPTHVEALEASLLPYLDREINALNPIELTVLRIGSFELFHCPEIP
                     YKVILDESVSLTKEFGSQEGYRYVNGVLNNLAKQVRSVEVSLDNE"
     gene            complement(7855..8322)
                     /gene="nrdR"
                     /locus_tag="U6309_002033"
     CDS             complement(7855..8322)
                     /gene="nrdR"
                     /locus_tag="U6309_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEN5505471.1"
                     /translation="MYCPFCHAEETKVVDSRLVADGAQVRRRRECLECHERFTTFETA
                     ELIMPLIIKRDGRREPFHIDNLRSGMLRALEKRPVSVDDLEKAIISITEEIRRRGERE
                     IDSQVVGELVMKELFRLDHVAYVRFASVYKRFKDVSDFRQTIDQMKNEDKEKS"
     gene            complement(8325..9578)
                     /locus_tag="U6309_002034"
     CDS             complement(8325..9578)
                     /locus_tag="U6309_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEN5505472.1"
                     /translation="MFDESYSIKNFDDVLFKAISDEKRRQEEHIELIASENYVSPRVL
                     EAQGSVLTNKYAEGYPGKRYYGGCEFVDVAEELAISRAKLLFGAHYVNVQPHSGSQAN
                     AAVMMALLSPGDTFMGMALPHGGHLTHGSKVNFSGKLYHSVEYGVDSNTGLIDYDALE
                     KLALQHKPKLIIAGFSAYSRILDWARFREIADKVGAYLMADIAHVAGLVAVGLYPSPV
                     PYADVVTTTTHKTLRGPRGGLILCKENEEIEKKLNSSVFPGMQGGPLMHVIAAKAVAF
                     AEALLPEFKTYQQQVLANARTMCSVLQSRGYDIVSGGTDNHLLLVDLINKGITGKEAD
                     AALGRANITVNKNSVPNDPRSPFVTSGLRLGTPAATTRGFKEREITLLSNWVADVLDN
                     VHDETNISRVKTQVLLLCREFPVYA"
     gene            complement(9606..9917)
                     /locus_tag="U6309_002035"
     CDS             complement(9606..9917)
                     /locus_tag="U6309_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEN5505473.1"
                     /translation="MRDSLKMLLVGSISMIIIACAASPLSESTGEYLDSSTTTAKVKA
                     SLVDQLGTTGFAVKVKTYKDQVQLSGFVDSQKIKQRAGIIAAGVDGVKSVRNDLIIKT
                     R"
     gene            complement(10042..10479)
                     /locus_tag="U6309_002036"
     CDS             complement(10042..10479)
                     /locus_tag="U6309_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505474.1"
                     /translation="MKYLIPCVVLFPLLSSCTVIQEDFYQPEYLGPAPRVEVTPSYPQ
                     GHYHRHYNQRYHSNNRYYSAPRGQVHYEHAKTQGHAAVVTPPPSQARVEVQKNVHGHN
                     ANNGQVHGHAHDNGNVHSHPDSSNVHGHDTSNPEEQKKIHGHS"
BASE COUNT         3291 a         2321 c         1917 g         3147 t
ORIGIN      
        1 taagggagcg gataaatgca aaaataaaaa cttctccaat taacctgact accatcctct
       61 tcatctgtag ccatatcaat gccctctaag ctacaattcc tatatacgaa ttaattgaca
      121 gattttcatg aatgcaccct tgacagtact gatggctcaa attaatccaa cagttggagc
      181 tattgacgcg aatacaaata aaattcttga tgtcatcaaa aaccatcagg caagccatga
      241 tgtgattatt tttccagaat taactctatc cggttatccg gcggaagatt tactctttcg
      301 taaagaatat catgataaaa tactgatcaa cctccatgat attcaggata ctaccaagga
      361 ttgttatgtc attgtcggac atccaatgat gcacattggt gattgctaca atggcttcag
      421 catcttttat caaggagaaa aaatcagaga atatcacaag caaaaattgc caaattatgg
      481 agtttttgat gaagcaagat attttactcc cggtaaaaaa gatccttgtg ttttatcaat
      541 taaaaatcat aaattagggt tttgcgtatg tgaagattta tggcaaaaag gtcctgtaga
      601 cgatttactt gacagtggca tttccatttt aatcagttta aacgcctccc cctttgatta
      661 tcgtaaatac cagctgagag aagagctttt aagatcttat gctaaacagg gggtgtccat
      721 tatttatgtc aatcaaatag gtggtcagga cgatttgctt tttgatgggc aatctttagc
      781 catggataat caaggtatta tccgtgccct ggctcccgcc tttgaagaaa gcttatgtac
      841 tgtcaatata gacggtaaca aaatagacgg atcgatcacc ccttatcttg acaaagaagc
      901 acttatttat aaggcactgg tttgtggtac cagagattac gtcagaaaaa acaatttccc
      961 cggcgtactg ctcgggctct ccggaggcat tgactctgcc ttaacgttag caatagctgt
     1021 cgatgcgctc ggagcagagc aagtccatgc tgttctgatg ccctcacgat ataccgcgtc
     1081 aatcagcaat gaagatgcac tggaacaatt aagcaacctt aaagttgcac attccattat
     1141 accgattgaa cccatgtttc aatctatgat tgaatcgctt catcctgttt ttaaagactt
     1201 gccaccggat accacggaag aaaatattca ggcaagaata cgtgggatgc tgctcatggc
     1261 tttgtcaaat aaaacaggga aaatggttct tactaccagt aacaagagtg aaacagctgt
     1321 aggttatgcc actttatatg gcgacatggc cggcggattt gccgtattaa aagatgtact
     1381 gaaaactcaa gtgtatgaat tagtgaggta tagaaacaag atttcattgg ttatcccaga
     1441 gcgagtcatt accagacccc cttctgctga attaaaacca aaccagacag atcaggatag
     1501 tttacccgaa tacggtgaac tggatgccat tatcatggct tacatggaac agaatttaag
     1561 tccctctgaa atcattgcca aaggatttaa acctcaagta gtcaacaaaa tcattcaatt
     1621 gattaaacgt aatgaataca agagacgtca ggctgcacca ggaataaaga taagtaatct
     1681 ggcctttggt aaagactgga gatatcctat cactaatggt tttaatcaat gcatttgaat
     1741 gcaataactg ccttgaaata aaattcaagg cagttattgc atttattaat taaatagatc
     1801 ttatagagag actatcattt ggttttgatt gttgtccttc gtgttgctcc atgtttttcg
     1861 ctctgttacc gaacaaggtg gcaatattct catatttatt tagattaatt ttaccaggtt
     1921 cttgtattgc agccttttct tcttgccttt cacttaactg gcttctcaaa caccgttcaa
     1981 cgtctccaaa taagcgcaaa tccagttttt tggcattcgt cgtcttggtt gcgccactgg
     2041 caggaggtgt tttagatttt ggtccggcag ccagacattc ctgcaattga gctttaaatt
     2101 ccttcatgtc ttccttgaca tcaatggact tgacagtatt tacattcagt aaataagtct
     2161 taagttgagg aatgtgctct tcctttaacg gatgactttg atcatttaac catgttttca
     2221 tagatgcata ggattctaat gaaatttcat gtgcgaaacc taatgtattt atccaccaca
     2281 atgaatattc attaggttta ccgcgcaaaa caaaagtagc agaagtccca ttttctttta
     2341 atacatcatt agtcaactca gcttgagttc tcggatcttt cggattgatg agcagttgaa
     2401 ctcgtcctaa aagcatttct cgggctttta cacactcctg tttaagctgc ttcaaatgca
     2461 aagcacttag attatcaata tccttaacga tatcctgaat ctttccgtct attagaattt
     2521 ggattaactc ttccccgggt tccaaagcat agactttgcc tgttgcatct acccaggaaa
     2581 gcttccaaat atcactttca aaaattaagc aaaattgtat aacaccctta ttttctttgg
     2641 ctgatttttt agctgcttcc aggtcattgg taattgaaac attatcgata aatttctgag
     2701 cactttgatg tgtctgtaac aattcctgtt ttcgagcttc tattaatttt tgggtagcca
     2761 ggtattgcga cataaactcg tcaattcttt tatttatttt ttgtctgagc ttcaaaataa
     2821 ttggatggac taactcactt tctaatggtt tatagccagg aactgacttg ataagctcat
     2881 caaataaata ataattaaac gatccatgtt cccactcccc tttcccaacc aataacttaa
     2941 actcctgcaa gcgagtgtag gcatcaggga acgcttccat gcctttaatt gccatatcaa
     3001 tttcttgtcc gcgttttctg gtaaactggt aaaaccaacc tttaagccat gattcaatgg
     3061 gcttgttata tcttgcccgg gcagattcta agacatgaac taattgctta cacccttcat
     3121 cggattcatc cgcaatccga cttaataact tttcggcaaa ttcgtttaaa ttgtcaatta
     3181 tatccaataa ttcttttcca tcctttggtg gcatatacgc tccttgttcc ttgtccaaat
     3241 ttcatcttac cttaatcaga attttataca agcaacaaaa tcattgagct aactggaatt
     3301 taaaatttaa attgctcaat aaagcgcaat agaaaacttg caaatttaag acaaaccgct
     3361 aacatgaatc agttaaaaaa taataataat aaaaagggat gtcatgagaa ttacattact
     3421 tacaacagct atattagcta ctggtgttgc ctccgccgca gtacctgtag acgggtggta
     3481 tgccagcgca tttggaggtt acacctatat tcctcccaac atatacaatt tcgcttatgg
     3541 ggtattccgt gatgaagtgg gatacaataa cggatataac gccggaggac gagtaggcta
     3601 ccaaagcaac ccaatccgct atgaagtaga atacacctat ctcaggggtg aagccagaaa
     3661 gtttaatctt aactttataa cacagctcgg tgttaccggt tttacctccg ccaatactat
     3721 catggccaat atttactatg attgccctga tatgctgcca gccatagcgc catttattgg
     3781 attggggatt ggttatgcct ctcttaggac agagcttgac agcataggtc cttttagacc
     3841 gtcttacttt accaccagcg acagcgcctt cgcctaccag ggaactgtgg gaataactta
     3901 taatttctcc gaaaattatg cggcaaattt ggcttaccgc tatatcgcaa ccgataattc
     3961 gagtaatttt ggaaaaacct accaggctca tttggccagc gtgggagcaa tctaccgttt
     4021 tgatcaagga aattataagt aaggatacgc aatgaagaag ttaattcaac tctcggtatt
     4081 atccggtttg ctattaagcg gttcaacttt tgctttcaac cagcctcaag gcttctacgt
     4141 tgggatattg ggggaaataa gtcatgggcc ttctggtgag gaccttatat ttaccgaacc
     4201 caaccgaacc attactgcca acgtggaata tagtcctgtt tctggaggtg gaggggctgt
     4261 attaggttat aaaataagcc actatcgcat tgaaggtgag gtactatata accggatttc
     4321 aactggtcct ttcacaataa gttcctgtac tttacaaagt cctaatgtcc taactccaac
     4381 aggcgtttgt ccttccgctt ttgaagttaa tcgactaggt tatgatggca gtagtgcagc
     4441 tctctatggt ttgctcaatc tgtattatga ttttttcagt tctgtgaatg aaagtactta
     4501 tgtaccttat attggaatag gcgcaggaca agtacgtatt caaaatagaa ataatttcgt
     4561 aaaaacaaca cccgtcaatc tgccggcttc agctggattt agcaccacca tggtctcagg
     4621 agctggacag ggcattgtag gcgtagggta tctaatggat gatttcgcct ggcttgctat
     4681 ggacttccgc tttctcacga ccagtagcct gtctgacttt aacaacagtc gctatacact
     4741 cagcacattc aatgtgggtg ttaactttac ctttgacaag tcaacctgat gacttcttgt
     4801 ggccttgggg ttcctgccac aaggtcacaa acaaaaccat caccataggc cctagaaaca
     4861 ggccaagcaa acccaaagtt tccactcctc ccaaaatgcc gaacaaaaca gcaaggaaag
     4921 gtaattgtat cgctccaccg ataagaactg gctttacaaa atgatcagca acaaacataa
     4981 ccaaagttcc ccataccaga acaatgatgc ctccaatcaa actgccaacg gaaagtaaaa
     5041 tcaaagcgac tatgataaaa acaatgggaa caacaaaagg tatcatagct gccaatgcgg
     5101 taataaaccc agtcaatgta ggggctggaa acccaaccaa ggcatagcaa atgcccatta
     5161 atatacctac ccctatgcca acaacaatag ttccattcac agtacctcgc aacgcgctgg
     5221 gaagcctgtc ggaataacgg aaccatctgt cacccaaaca atattctcct atatgctgaa
     5281 tttgtaatag taatttatcc ccatcacgat agaagaaaaa aagtgttaaa agggtaaatc
     5341 ctacttgaaa actacgatga gccaaattga ccccaatctg cttgatgtaa taactggtcg
     5401 gggttaacgt tacatgcaaa ttagataaaa actccttgat acttcccggc ttaccaatat
     5461 tcacatccca atattgaatt aaatcatccc caaccaatgg aatattttta aaaaactcag
     5521 gagctgcccc accttcctta ttaatatgct gcaaaaaatt aataaataat tgagactctt
     5581 tgataaggat acctataagc caactcaagg gaataaggaa aagcaagccc atcagtgttg
     5641 taaataagaa cgctgatgtg ttgtgtttgt taccaaataa tttacgccat ctggtgtata
     5701 agggataggt agctataaca ataatagcgg cccatatcat ggaaggaatg aatttatgaa
     5761 caatgtaaag cgagaagata acgatactga ctgttaaccc tatgctaatt agctctttat
     5821 gattttcatt cattaagcaa agctccaaac caggatttgc ataatacacc aggcaggaac
     5881 tgctgctaaa acgtcgtcca gcataattcc taacccaccg tgaactcttt gatcgatcat
     5941 acgtattggt tggggtttcc agatatcaaa aatacggaac aaaataaagc caattaacat
     6001 ccatgatatt cctttgggag ctaaaaacat agttaataaa taaccaacca cttcatccca
     6061 tacaattcct ttgtaatcat gtaccccaag atcttctgtc actctgtcac tgacccaaac
     6121 tcccagcgca aatgccaggg cagtccataa taaataaatg ctccaatgag cattgatcat
     6181 taataaatag aggggaatag cagctaatgt accccacgtg cctggagcaa ttgacatcaa
     6241 tccactgcca aaaccaaaag cgataaaata aacgggatct tgccatactc gatttgccaa
     6301 attgacttgg ttcatatttg ctcctcaacc taaatatcgc tatcacatca ctttagaatc
     6361 gaattttgtc tttaattttt attatcatac tatagaggcg ggatttaaaa atgtgaatag
     6421 cctctgggtt gcaattcttt acttggacta tcagctgatt gtattcttaa tcctggtttc
     6481 tcttctatca caccgatggg aaaacagatc agattttctt tttgaagatc ccgattaaaa
     6541 gaatccagct gttgtgttga aactgtaaaa cataactcgt aatcatcacc tcctgaaaga
     6601 gccaagtcaa gggcttgttc aggacaatat tgattcaata aaggatgcct gggtattgct
     6661 tcttcaaaca agttggcccc aacaccactt gcgacgcaaa tatgatttaa atcagcgcct
     6721 aacccatcag agatatcaat cgctgaagta gcatattttc taagcaaatg gcataaatcc
     6781 acccgtggtt taggatgtaa tagtttattc attaattcta ttttatcttt tgcattcact
     6841 tttttatgcc catccagcaa ttttacagcc agacccgcag cccccaattc accggaaact
     6901 ataatcgcgt cacccgcttg tgcgccacaa cgtctgattg cttgtccctg aggtgtgagc
     6961 cccatgatgg tcaaagtgat tgataacggg cctttggtcg tgtcaccacc gatcagatcc
     7021 acattatata agccaagaga gtcttttatc cctctcgcga aggaatcaag ccaactctga
     7081 ttcaattcgg gcaaggtcaa tgcaagagtc acccaacatg gctgtgcgcc catggcagcc
     7141 atgtcactaa tattgaccat tactgccttg caggcaatat catagggatc ccattgcggt
     7201 agaaaatgaa tgcctgaaac caaagtatcc gtgctgacta gcaaatgcat tccatcggga
     7261 acctgcaagc aagcagcatc atcccctatc ccaaaaagga catcatttct cttgcatggg
     7321 atggatttaa aataatgatc aatcagggta aattcattca ttatcaagac tgacttcaac
     7381 agagcgaacc tgttttgcca aattattcaa taccccatta acgtaacgat atccctcttg
     7441 tgaaccaaac tctttagtca aggatactga ttcgtcaaga atgaccttat aaggtatctc
     7501 agggcaatga aacaattcaa aagatccaat tctcaataca gtaagctcta ttggattcaa
     7561 tgcattgatt tctctatcta aatatggcag taaacttgct tccaaagctt ccacatgagt
     7621 tggaatgccg tataacaggc ggcagaaata ttctccatct actttatcca tattattaat
     7681 tgttctgaat tgagcttcaa tttcatgaag atctgtacca gacattagcc attgataaag
     7741 ggcttgcaat gcaaattttc gagctcgtct tttaccacga attgattgtt tttccacaat
     7801 tacctcatat tattctaata acaacaagaa aagtgagccc ttcacatttt ttatctaaga
     7861 cttttcctta tcttcatttt tcatttgatc aattgtttgt ctaaaatcac tcacatcttt
     7921 aaagcgttta tacactgatg caaaacgcac ataggccaca tgatcaagcc tgaataattc
     7981 cttcattacc aactcgccaa ccacctggga atcgatctcg cgttcacctc ttcttcgaat
     8041 ttcctcagtg atagaaataa tagctttttc caaatcatca acgctgacag gtcttttctc
     8101 caaggctctc aacatgccag aacgtaaatt atcaatatga aacggttctc ttcttccatc
     8161 acgttttata atcaatggca taatcaattc agcggtttca aaagtagtaa accgctcatg
     8221 acattccaga cattcacgcc ttctgcgaac ttgagcacca tccgcgacca gacgtgaatc
     8281 cactaccttt gtttcttccg catgacaaaa tggacaatac atgattaggc ataaaccgga
     8341 aattcacgac agagtagcaa cacctgggtt ttcactcttg aaatattagt ttcatcatga
     8401 acattatcaa ggacatccgc tacccagtta gataacaaag taatctctct ttctttaaag
     8461 cctcttgtgg ttgcagcagg cgtacccaaa cgtaaaccac tcgttacaaa aggcgagcga
     8521 ggatcattgg gaaccgaatt tttattgact gttatgttag ctctgcctaa agcagcgtca
     8581 gcttctttac ctgtaattcc tttatttatc aaatccacta gcaaaagatg attatcagtt
     8641 ccgccagaaa caatatcata gcctctgctt tgtaacacac tgcacatcgt tctggcatta
     8701 gctaaaacct gttgctgata agttttaaat tcgggtaaca gagcttctgc aaaagcaact
     8761 gccttggctg caattacatg cattaagggc ccaccttgca taccaggaaa tacagaagaa
     8821 tttaattttt tctcaatttc ttcgttttcc ttacaaagga ttaaaccacc gcgaggtcct
     8881 cttaaagttt tatgagttgt cgttgtcaca acatccgcat aaggaacagg agaaggatat
     8941 aaccctacag ccaccagacc agctacatga gcgatgtccg ccattaaata agcgccgacc
     9001 ttatcggcaa tttctctaaa tcgagcccaa tccaggatcc tggaataagc tgaaaaaccg
     9061 gcaatgatca attttggttt gtgttgaagc gcaagttttt ccagagcatc ataatcaatc
     9121 aaacccgtat tgctatcaac accatactcg acagaatggt ataatttacc tgaaaaatta
     9181 acctttgacc catgagtcaa gtgcccacca tgaggtaaag ccatgcccat aaaagtatcg
     9241 cctggggaca ataatgccat catgactgct gcgttcgctt gtgatcccga atggggttgc
     9301 acattgacat aatgagcgcc aaaaagtaat tttgcccttg aaatggctaa ctcttcagcg
     9361 acatcaacaa actcacaacc tccgtaatag cgtttgcctg gatatccttc agcgtattta
     9421 ttcgtcaaca cagaaccctg agcctccaat actctcgggc tgacataatt ttcagatgct
     9481 attaattcaa tgtgttcttc ttggcgtcgt ttttcatcag aaattgcttt aaaaagaaca
     9541 tcatcaaaat tttttatact ataactttcg tcaaacatga atgttccttg gttggaaggt
     9601 tatatttacc ttgtttttat aataaggtca ttacgaacac tctttacacc atctacgcca
     9661 gccgcaataa tcccagctct ttgttttatt ttttgagaat cgacaaaacc gctcaactgc
     9721 acttgatctt tatatgtttt tacttttacg gcaaatcccg tagtccccaa ttggtcaacc
     9781 aaactggctt tcaccttggc agtagtcgtt gaactatcaa gatattcccc tgtactttcc
     9841 gacagaggag aagcggcaca cgcaataatt atcatggata tggatcccac taataacatc
     9901 ttaagtgagt cgcgcataga ggctccttaa agataaaaac gttattagag tatcataaaa
     9961 attacccgga tacgaagagg ataatagtac aattttagtt cttttaaaaa aaaagtttcc
    10021 tgtattcagc aagttaaatt attaactatg gccatgaatt tttttctgtt cctcaggatt
    10081 ggaagtatca tgtccatgta cattagaaga atcaggatgg ctgtgcacat taccattatc
    10141 atgagcatgt ccatgcacct gaccgttatt tgcattgtgt ccgtgaacat ttttctgaac
    10201 ttcaactctg gcttgtgatg gtggcggagt aactaccgca gcatggcctt gagttttcgc
    10261 atgctcataa tgaacctgcc cccttggcgc actgtagtat cgattattac tatgatatcg
    10321 ctgattgtaa tgacgatgat aatgcccttg aggataagag ggggtaacct ctacgcgagg
    10381 agcaggcccc aaatactctg gctgataaaa gtcttcttgt ataactgtgc aggaagaaag
    10441 taaaggaaac aatactacgc agggaatgag gtatttcatg tacaactcct caaaggaaag
    10501 gaaaactctc gaatcattga gagctttccc gaattttcaa tgaattatct ggttgataac
    10561 tcagccttag cttctttcaa gtaaggaact acacggatgg tactgttcac atttgtccaa
    10621 ccggaataaa tagtatagta aggtgatgta atagtaatgt acataccact atggca
//
LOCUS       DASLOE010000105        10529 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.106, whole genome shotgun sequence.
ACCESSION   DASLOE010000105 DASLOE010000000
VERSION     DASLOE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.106"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            111..1757
                     /locus_tag="U6309_002037"
     CDS             111..1757
                     /locus_tag="U6309_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN5505475.1"
                     /translation="MKRYSKRLLTYIYLLMKSISLAFTMQQLSYSTDKIPIPINKLPD
                     DLNKVMNKSIYKKALWGLRVVDLDTGNVLMNLNSSTQFYIGSVRKIFSVGELLNERGA
                     DYQSVTTVHYDGVVHNGNLNGNLVLVASGDLTMGGRTLPDGHVAITEYDHNEANSLGN
                     AIITQTNLLAGYQNLAKQVYKAGIRSISGDIVIDDRLFEPFNFRGEFDVTPIFINDDV
                     VDIVILPSLINENANVSWQPHSSAFTVINQLLTVNTHEKYNLELESKLPACIGKPGCS
                     GIIKGHLPINFIPPLTHNYPLVQTFRITKPSNYARTIFIEALQEAGINVSSIDKTKEN
                     PKKLLKSNNLYNKNNKIAALKSLPLGEHVKYILKVSYNIGADTSLVLYGLTKGARSMI
                     ESLNVEKKVLDKKYGISPSEYHFIDGSGGGETKATNTAIIKWLTIMSKRPVFNIFYNS
                     LPILAVDGSLGSINNFQSDPSLSGATGRVHAKTGTYVLVNESGMLVKGRSLAGYIISK
                     KNHRLAFALVVNNIPIQSLEDLFAVAQDQGIIASILWRDY"
     gene            complement(2075..3397)
                     /gene="bioA"
                     /locus_tag="U6309_002038"
     CDS             complement(2075..3397)
                     /gene="bioA"
                     /locus_tag="U6309_002038"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEN5505476.1"
                     /translation="MVNTDQLIAKDLKHIWHPCSQMKDFETFPPIVVNKAQGSYLYTN
                     KGPVIDAISSWWCKSLGHGHPAVIAAIKNQLDCFEHVISANTTHPAAVELAEKLSEIT
                     GKQHVFFASDGSSAVEIAMKLAIHASQIKGHKNKNQFIALKNGYHGETIGTMSVSDLG
                     LYKTPYASFGVQCHFIDDIPYVSGLKDPLWTNCDAYWNNVVQQLNKICDKVCAILLEP
                     IIQGAGGMLCYSADFLSKISSWAKEKDIYLIADEIMTGIGRTGEWLACDHARVQPDLI
                     CLSKGLTSGSIPFSCVMIDSTLFDLFYDDYHTGKSFLHSHTYSGNPLAIGAALATIKT
                     IEQDNILMHSQELGKIMFSYLQDIARDSRKLVNVRGVGAVVAADLIESGQARIGFKIY
                     QEAIKQGALIRPIGNTLYWLPPLNTDKKIIGKLAEITLNSIEKAYNKA"
     gene            3470..4417
                     /gene="bioB"
                     /locus_tag="U6309_002039"
     CDS             3470..4417
                     /gene="bioB"
                     /locus_tag="U6309_002039"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEN5505477.1"
                     /translation="MSEENKQWSISDIEAIYQQPFNDLLYQAHTIHRTYHDPNSLQFA
                     TLLSIKTGACPEDCGYCSQSGHYKTHVEKEKLMSVEEVLQCAKEAKEGGAKRFCMGAA
                     WRCPPDKAIPQLKEMIEGVKSLGLETCMTLGMLTKEQASHLKEAGLDYYNHNIDTSPS
                     YYDKVVTTRKFSDRLDTLNNVRSAGINVCCGGILGLGETREDRIEFLLTLANMETPPE
                     SVPINRLIPVEGTPLAQAERVEGIELVRTIATARILMPKSAIRLTAGRTEMSDELQAL
                     CYFAGANSVFIGDKLLTEDNPQRFKDKTLFNKLGLTEMV"
     gene            4418..5560
                     /locus_tag="U6309_002040"
     CDS             4418..5560
                     /locus_tag="U6309_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEN5505478.1"
                     /translation="MTLNIKINEYTAQLQEQGLLRTRQILETDKIDYIRFDSNDYLSL
                     TEDRRIAEAYQKGYELYPCGSGASMVLSGYHSSHQTLERAFAEMLGVDDCLLFSSGYC
                     ANLAITALLGRLGTHCFIDKSVHASIYDGLALSRVTYSRYIHNDMNALSALLKSHLGD
                     SVLITEGIFSMSGQIAPLSTISTLCKENSSELFVDEAHAFGVIGPQGMGSVPGHGLTQ
                     NEVPLRVIPFGKAFASQGAVVGGRKDWINALLQTARSFVYSTAISPALCYGLLKTLEI
                     IAAADSRREKLNQLVAQFKELIKSSPLNWLDSDTPIQQLRLGCPHKALHYASELKKNG
                     IYCSAIRAPTVNSASSGLRIIINYKHTIEQIIELFNKLHLIHEYSS"
     gene            5544..6263
                     /locus_tag="U6309_002041"
     CDS             5544..6263
                     /locus_tag="U6309_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN5505479.1"
                     /translation="MNIHLDKHGQGMPLVLFHGWGFDSQIWQPIIPYLKPKYQIILVD
                     LPGFGLTPMMDWESFKKNLLDQLPDKFALAGWSMGGLYATRLAIEEPARVQYLINITS
                     SPRFISDIDWPGVAEEVFVNFYNNLSKDINKTLKEFISLQLNKMKFDFKIGNPPSPEG
                     LAFGLEILGTWDFREQLKQISIPTVYLFGRLDPITPVKTMAIMEKNYPNFKYVLFNRA
                     AHMPFLSHTDLFIKMMDEFIK"
     gene            6260..6898
                     /gene="bioD"
                     /locus_tag="U6309_002042"
     CDS             6260..6898
                     /gene="bioD"
                     /locus_tag="U6309_002042"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEN5505480.1"
                     /translation="MKRYFITGTDTDCGKTFVTNQLVNYFSDSAAIKPIASGCEYSEN
                     QLVNSDALLHQQQNHLPLEIVNPWRFRLPVSPHLSARADGASIDVHKVADYCLNLQLN
                     DIKKLFIEGAGGLMVPLNEQDTWLDFLKLTRIPVILVVGMKLGCINHTLLTQEVLEIN
                     KIKCQGWIANCLDQDMLMLDENINTLEAKLKYPLLARTNYGGKISDICLSSL"
     gene            7033..7347
                     /locus_tag="U6309_002043"
     CDS             7033..7347
                     /locus_tag="U6309_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEN5505481.1"
                     /translation="MPIYEYQCTNCHHQFDLMQKVTDAPVKQCPKCYENTVTKLISPA
                     GFQLKGTGWYVTDFKNKGNGPKESTENKSGDPGINKDNSSKETPAASSADSAKSTKGD
                     SE"
     gene            7344..7973
                     /locus_tag="U6309_002044"
     CDS             7344..7973
                     /locus_tag="U6309_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEN5505482.1"
                     /translation="MKLISLRAYLITGLIVWLPIFVTIVVLRFIIDMLDSTLALFPKA
                     YQPEQLFGFYIPGFGVLFSLVLLLVTGIIATNFLGQRLVSRGESLLAKIPLVRSIYNA
                     VKQVIHAVLSTNSQAFRKVVLVEYPRKGLWTIAFQTGSVNPEIKEKSKEDMMSVFVPT
                     TPNPTSGFMLMIPRQDAIELNMSIDEALKLVISLGVMQSDPALAQVKTQ"
     gene            7992..9773
                     /gene="aspS"
                     /locus_tag="U6309_002045"
     CDS             7992..9773
                     /gene="aspS"
                     /locus_tag="U6309_002045"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEN5505483.1"
                     /translation="MRTHYCSAVNELSLDKQITVCGWVHNRRDHGGVIFLDIRDRSGL
                     LQVVYEPENKEIFAIAEKLRSEFVVRVTGIVRKRPEGMINDKMETGRVEVIGTQLEIL
                     NQSPTPPFLPDDHQIINEDLRYKYRYIDLRRAVMQKKLTLRHKLNSCIRNYLNEQNFL
                     DIETPMLTKATPEGARDYLVPSRVHPGQFYALPQSPQLFKQLLMMSGFDKYYQIVRCF
                     RDEDLRADRQPEFTQLDIEMAFINEEDILQLIEGLLKVVFKEILNITLPDKLPRMSYK
                     EAMTRYGSDKPDLRNPLELIDIADLVKDCDFNVFASAANDNSGRVVALKLPNGCDLSR
                     KDLDNYGQFVTIYGAKGLAYIKVNDLSAGMAGLQSPILKFLSETAVQSILNRVEAQTG
                     DVIFFGADKAHVVNESMGALRNKLGHDRNLINPGWQLLWVVDWPMFELDPQSNKLQPM
                     HHPFTSPQELSAEALRSKPTQTLAKAYDIVINGYEIGGGSIRIHQPELQKTVFDLIGI
                     DEQEAHEKFGFLLDALQYGAPPHGGIALGIDRLAMLLTDSTSIRDVIAFPKTQTASCP
                     LTSAPSPAGNAQLTELGIRLAPTITTK"
     gene            9748..>10529
                     /locus_tag="U6309_002046"
     CDS             9748..>10529
                     /locus_tag="U6309_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HEN5505484.1"
                     /translation="MRQLLRQSNLNLSTTSFKYLWSFFLPLFLLLFSFSLFSSSVKEN
                     NATLVEYLAHEKTHLTIAINEIKQKSAPKDEQEYNEKLRQIRTILSMNKAKIESLNSF
                     SENQKKEEVNLNDQLKRLQQMPLANAEVTIQEKVGKIETLLTVNKKTTELIAETLDLA
                     KQYQLLLNDILNELKLWKANFDLKEKLKSIKAIRAKLNKELNILYENAVSSQDQKQQK
                     LTISEQIQYEANLYINNQRIAVIHQKLNALELQKKVIKTNIL"
BASE COUNT         3345 a         1819 c         2102 g         3263 t
ORIGIN      
        1 tatagagttt aatgtgattt ctgctaattt cccaataatt tttaacagtt attatgacaa
       61 attggacaaa cctaaaagaa taaaaacata tattactgga aatgcgaatg atgaagagat
      121 actcgaaaag attactgact tatatttatc tacttatgaa aagcatatct ctagcgttca
      181 cgatgcaaca actatcatac agcacagata aaattcctat accaatcaat aaactacctg
      241 atgacctaaa caaagtgatg aataaatcta tttataaaaa agcattgtgg gggttacgtg
      301 ttgttgatct tgatactggt aatgtattga tgaatctcaa ctcaagtacg cagttttaca
      361 ttggttctgt gcgcaagata tttagtgtag gcgaactatt gaatgaaaga ggagctgatt
      421 atcaatctgt tactacagtc cattatgatg gtgtagtaca caatggtaat ctgaatggga
      481 atctggtact cgttgcttca ggtgatttga ccatgggagg acgaacttta cctgatggac
      541 atgtcgctat cactgaatac gatcataacg aggcaaattc attgggtaat gcaattatta
      601 cacagactaa tcttttagca ggttatcaaa atctcgctaa acaagtttac aaagctggca
      661 ttcgctctat ttcaggcgac attgtgatag acgatagatt gtttgaacca tttaatttca
      721 ggggtgaatt tgatgtaaca cctattttta taaacgatga tgtagttgat attgtaattc
      781 ttccaagttt gataaacgag aatgcaaatg tatcgtggca gccacattca tcggcgttca
      841 ccgttatcaa ccaacttctt acggttaata cgcatgaaaa atataatctt gaacttgagt
      901 caaaattacc tgcctgcata ggtaaaccag gctgttctgg catcattaaa ggccatttgc
      961 ctatcaattt tataccgcca cttactcaca actacccatt agtacaaact tttcgaatca
     1021 ctaaaccatc taattatgcc agaacaattt ttatcgaggc actacaagaa gcaggcatca
     1081 atgtttcatc gattgataaa accaaagaaa atccgaaaaa actactaaaa tctaataatt
     1141 tgtacaataa gaataacaaa attgctgctt tgaaatcatt gccattggga gagcatgtaa
     1201 aatatattct caaagttagt tataatatcg gagcagatac cagcctggtg ttgtacggac
     1261 taacaaaagg cgcgcgctcc atgattgaaa gtctaaacgt ggaaaaaaaa gtcctggata
     1321 aaaaatatgg catatcccca tcagaatatc attttattga tggcagcggt ggcggcgaaa
     1381 cgaaagcaac caataccgct ataataaaat ggcttaccat aatgtctaaa agaccagtat
     1441 ttaacatatt ttacaatagt ctgcctattc ttgcagtaga tggttcactt ggctcaatta
     1501 ataactttca atcagaccca tcgctttctg gggcgacagg tcgagtgcat gcaaaaactg
     1561 gaacttatgt attggtaaat gagtcgggta tgttagttaa ggggcgctct cttgctggtt
     1621 acattattag taaaaaaaat caccgacttg catttgcctt agttgttaat aatataccta
     1681 tacaatcact agaagattta tttgctgtag ctcaggatca aggcataata gcatcaattc
     1741 tatggcgaga ttattgatta ttcatataat aattcattca acgattgaaa gatattatcg
     1801 aaattgagat agttacaaaa tgcatatttg tctaatttat tgtccactca ataatttaag
     1861 taatttcaat aaactttatt aaatacacat tgtatctcgc tgtgtttaat tttatcgtca
     1921 acatagtgcc cagtttagat tcaaaccaaa caagagttag gttagcaggt tttaggcaaa
     1981 ttacttggtt ttgggccaag gagattgcta ttctttggac aattggcgag cagatttgtc
     2041 tttaaactat ttgtgaataa ccttactcat atccttaagc tttgttatag gctttttcta
     2101 tagagtttaa tgtgatttct gctaatttcc caataatttt tttatctgtg ttaagaggtg
     2161 gtaaccaata gagagtattt ccaattgggc gaatcagtgc gccttgtttt attgcttcct
     2221 gataaatttt aaatccaatt cttgcttgac cgctctctat gagatcagcc gcaacaactg
     2281 ctccaacacc acgaacattt acaagttttc tactgtccct tgctatatcc tgcagatagg
     2341 aaaacatgat tttaccaagt tcctgactat gcatcagtat attatcttgt tctattgttt
     2401 taatagttgc caaagcagcg cctatagcaa gtggattacc actataggta tgtgaatgca
     2461 aaaaagattt ccctgtatgg taatcatcat aaaaaagatc aaataaggta ctgtcaatca
     2521 tgacacagct aaatggtatt gagccggaag ttagtccttt ggatagacaa atcagatcag
     2581 gttgtaccct tgcatgatca caggctagcc attccccagt tctgcctata ccagtcataa
     2641 tctcatcagc aattaaataa atgtcttttt ccttagccca tgacgagatt tttgagagaa
     2701 aatctgcact gtaacatagc atacctccag caccctgaat gataggctcc agaagtatcg
     2761 cgcaaacttt atcgcaaatt ttattcaatt gttgtacgac gttattccaa taagcatcac
     2821 aatttgtcca taagggatcc ttaaggcctg agacataggg aatatcatcg atgaaatggc
     2881 attgcacacc aaacgatgca tagggagtct tatagagtcc taaatcactg acactcatgg
     2941 tgccaatagt ttctccgtgg tagccatttt ttaaggcaat gaattggttt ttatttttgt
     3001 gcccttttat ttgtgaagca tgaatggcta atttcatggc aatttcaaca gcactggaac
     3061 catcactggc aaaaaacaca tgctgttttc ctgtaatttc acttaatttt tcagctaatt
     3121 ccactgcagc agggtgagtt gtattggcac taatcacatg ctcaaaacag tccagttggt
     3181 ttttgatagc agctatcact gctggatgac catgccctaa tgatttgcac caccaactgg
     3241 aaattgcatc aatcacgggg cccttattgg tatagagata gctaccttgt gctttgttga
     3301 caacgatagg ggggaatgtt tcgaaatctt tcatttgtga acaaggatgc caaatgtgct
     3361 ttaagtcttt tgcaataagt tgatctgtgt ttaccatttt aaaaattttg gttgacaagt
     3421 ataggcgcga ctttatcatg ttcactattc tgaattaaag ggggtagccg tgtctgaaga
     3481 aaataaacag tggtcaattt ctgatattga agcaatttat caacaaccat ttaatgattt
     3541 gctttatcaa gcacatacca ttcataggac atatcacgat cctaattctt tacaatttgc
     3601 aaccttgtta agtattaaaa cgggcgcatg cccagaagat tgtggctatt gctctcaaag
     3661 tggtcactat aaaacgcatg tagaaaaaga aaaattgatg tcagttgaag aggtattgca
     3721 atgtgccaaa gaagcaaagg agggtggggc caaacgtttt tgtatgggag ctgcatggcg
     3781 ttgtccacct gataaagcaa ttccgcagct aaaagaaatg atagaaggag taaagtcttt
     3841 agggcttgaa acctgtatga ctttaggcat gcttaccaaa gaacaggcct ctcatttaaa
     3901 ggaggctgga ttggattatt ataaccataa tattgatacc tcaccatcct attacgataa
     3961 ggtggtaacc actcgaaaat tcagtgatcg attagatact ttaaataacg ttcgttcagc
     4021 gggaattaat gtatgttgtg gtggtatttt aggattgggg gaaaccagag aagatcgaat
     4081 cgaattttta ttaacattag ctaatatgga aactcctccg gaaagtgtgc caattaatcg
     4141 tttaattcca gtggaaggaa cacctttagc ccaagctgag agagtagaag ggattgaatt
     4201 ggtaagaacc attgctacag caagaatttt aatgcctaaa agtgctatta gattgacagc
     4261 cggtcgaacc gaaatgagcg acgagttaca agcactttgc tattttgccg gagccaattc
     4321 tgtgtttatt ggcgataaat tattgacaga agataatccc cagcgcttca aagacaaaac
     4381 tctttttaac aaactgggtt tgactgagat ggtataaatg actcttaata taaaaattaa
     4441 tgagtatact gcacagctgc aagaacaagg attattgcgt acaagacaaa ttttagaaac
     4501 ggataagata gattatattc gatttgactc taatgattat ttgtcgctca cagaagatag
     4561 gcgaattgct gaagcctatc aaaagggata tgagttgtat ccttgtggaa gtggtgcttc
     4621 gatggtttta agtggctacc atagtagcca ccaaaccctt gagagggcct ttgccgaaat
     4681 gctgggagta gatgattgtc ttttattttc ctctggctac tgtgcaaatt tggccattac
     4741 ggccttgctt gggcgtttgg gaacacattg ctttatagat aaaagcgttc atgcatcaat
     4801 ttatgatgga ttggctttgt cacgagtaac ctactctcgt tacatacata atgacatgaa
     4861 tgctttgtct gccttgttaa aatctcattt gggcgattct gttttgatta cagaaggtat
     4921 ttttagtatg agtgggcaga tagcgccgtt atcaacgatt agtacgttat gcaaagagaa
     4981 tagctctgag ctctttgtag atgaagcgca tgcttttggt gtgattggac cgcaaggaat
     5041 gggctctgtg ccaggccatg ggctgacaca gaatgaagtt cccttgcgag ttattccttt
     5101 tggtaaggca tttgcttccc agggcgcagt ggttggtggg cggaaagatt ggataaatgc
     5161 tttgttgcaa acggcacgtt cttttgttta ttcaactgct attagtcctg ctttatgcta
     5221 tggtttgctc aaaacattgg agataatagc agctgccgac tccagaagag aaaaattaaa
     5281 ccaattagtt gctcaattta aagaattaat aaagtcttca cctttaaatt ggctggattc
     5341 agatactcca attcagcaat taaggttagg atgccctcat aaggcactgc attatgccag
     5401 tgaactgaaa aaaaatggga tttattgctc tgctatcaga gctccaacgg taaatagtgc
     5461 ttcttccggt ttacggatta ttattaatta taagcatact attgaacaaa tcattgagtt
     5521 atttaataaa ttgcacttaa tacatgaata ttcatcttga taaacatggg caaggcatgc
     5581 ctttggtcct atttcatggc tggggatttg atagccaaat ctggcaaccg ataattcctt
     5641 accttaagcc taaatatcag attattttgg tcgatttgcc tggttttgga ttgactccaa
     5701 tgatggactg ggagagtttt aaaaagaatt tgcttgacca attacctgat aaatttgcat
     5761 tggcagggtg gtcaatgggt gggctgtacg cgacacgttt agcaattgaa gagcctgcca
     5821 gagtgcagta tttgataaat ataacgtctt cacctcgttt tatctctgat attgattggc
     5881 cgggagtagc agaggaggta tttgtcaatt tttataacaa tttatcgaaa gatattaata
     5941 agacattaaa agagttcata tccttgcaac tcaataagat gaaatttgat tttaaaatag
     6001 gtaatcctcc atctccagaa ggcttggcgt ttggtctcga aattttgggt acctgggatt
     6061 ttagagagca gttgaaacaa atatctatac ctactgttta cttatttggt cgacttgatc
     6121 cgattactcc tgtcaagacc atggcaatta tggagaaaaa ttacccgaat tttaaatatg
     6181 tcctcttcaa cagagcagct cacatgcctt ttctgtccca taccgattta tttattaaaa
     6241 tgatggatga atttataaaa tgaaacgata ttttattacg ggtactgaca ctgattgtgg
     6301 taagacattt gtaaccaatc aattggttaa ttatttttca gattcagcag cgataaaacc
     6361 catagcaagc ggatgtgagt acagtgaaaa tcaattagta aattctgatg cacttttaca
     6421 ccagcaacag aatcatttgc ctttagaaat tgttaatccc tggcgattta gattgccagt
     6481 ttcgccgcac ttatcagcaa gggcggatgg tgcaagtatt gatgtccata aagtggctga
     6541 ttattgcctt aatttgcaac tgaatgatat taagaaatta tttatcgaag gagccggggg
     6601 attaatggtt cctctgaatg agcaggatac ttggcttgat tttttaaaat taaccaggat
     6661 ccctgttatt ttagttgtgg gcatgaagtt gggttgcatc aaccacactt tgcttaccca
     6721 ggaagtgctc gaaataaata aaatcaaatg tcaaggatgg attgctaatt gcctggatca
     6781 ggacatgcta atgcttgatg aaaatattaa tactcttgaa gccaaattaa aatatccttt
     6841 attagccagg acaaactatg gcggcaagat ttctgatata tgtttaagct ccttgtaatt
     6901 gcagtaaaat tttatatcaa aacagcacta tatacctgat gttattgttt gttctgcttg
     6961 cgctataccc atggcagcaa ttataatgaa aagttaaatt acagatactt cgagtttatg
     7021 gagtgatgct agatgccaat ttatgaatat cagtgcacaa actgtcatca tcaatttgat
     7081 ttgatgcaaa aagttacgga tgcgcctgta aaacaatgcc caaagtgtta tgaaaatact
     7141 gtaacaaaac ttatttctcc ggctggattt cagttaaagg ggactggctg gtatgtcaca
     7201 gattttaaaa acaaaggaaa tggaccaaag gagtcaactg agaataaatc aggagatccc
     7261 ggcatcaata aagataattc ttctaaagaa actcccgcag caagctctgc agatagcgca
     7321 aaatcgacaa aaggtgatag cgagtgaaat taatctccct cagagcctat ttaattaccg
     7381 gattaatagt gtggttacca atatttgtaa ccattgtagt cctgcgattt attattgata
     7441 tgcttgatag taccttggct ttatttccaa aagcttatca acctgagcag ttattcggtt
     7501 tctatatacc aggctttggc gttttatttt cattggtttt attactcgtg acaggaatta
     7561 ttgcaaccaa ttttttgggg cagagacttg taagtcgggg tgaatcctta ttagctaaaa
     7621 tacctctggt gcggtcaatt tataatgcag taaagcaagt tattcatgct gttctatcaa
     7681 ctaatagcca agctttccgt aaagttgttt tagttgaata tccccgtaaa ggactttgga
     7741 ctatcgcatt tcagactggc tccgttaatc ctgaaattaa agagaaatcc aaggaagaca
     7801 tgatgtctgt atttgttcca actacaccta accctacttc gggttttatg ttgatgatac
     7861 ccaggcaaga tgccatcgag ttgaatatga gcattgatga agcgttaaaa ttagtaatat
     7921 cgttaggcgt aatgcagtct gatcctgcat tggcacaagt aaaaactcaa taaactttaa
     7981 taggtggttt tatgcgtaca cactactgtt cggccgtgaa tgaattaagc ttggataagc
     8041 aaatcacagt ttgtgggtgg gtgcataata gacgagatca tggcggggtt atctttttag
     8101 atattcgtga tcgttcagga ttattgcaag tggtgtacga gcctgaaaat aaggaaattt
     8161 ttgcaattgc tgaaaaacta agatcagagt ttgttgttcg tgttactggc atagtgcgta
     8221 aaagaccaga aggaatgatc aatgataaaa tggagactgg ccgggttgag gtaatcggta
     8281 cacaacttga aatccttaat caatctccaa ctccaccgtt tttgccagat gatcatcaaa
     8341 ttatcaatga agatttgcgc tataaatacc gctatattga tttgcggcgc gctgttatgc
     8401 agaagaaact gactttaaga cacaagttaa acagttgcat acgtaattac ttgaatgagc
     8461 agaacttttt agatatagaa acgcctatgt taactaaggc aactcccgaa ggcgcacggg
     8521 attatcttgt gccttcaagg gtgcatccag ggcagttcta tgctttacct caatcgccac
     8581 aattatttaa gcaattgttg atgatgtcag gatttgataa atattaccag attgtacgct
     8641 gttttagaga cgaagattta agagctgaca gacagcctga atttacgcag cttgatatag
     8701 aaatggcatt tattaatgaa gaagatatat tgcagctaat tgaaggattg cttaaggttg
     8761 tatttaagga aatccttaac attaccttac ctgataaatt gccaagaatg tcgtataaag
     8821 aggctatgac acgctatgga agtgataaac ctgatctgcg taatccactg gagttgattg
     8881 atattgcaga tttggtaaag gattgcgact ttaatgtatt tgcctctgct gcgaatgata
     8941 attcaggaag agtggttgct ttaaaattac ctaatggatg tgatcttagt cgaaaggatc
     9001 tggataacta tggacaattc gtaactatat atggggctaa aggacttgca tatattaaag
     9061 tcaacgattt aagtgctggt atggcgggtt tgcagtcacc aattctaaag tttttatctg
     9121 aaacggctgt gcaatctatt cttaatcggg ttgaggcgca gactggcgac gttatttttt
     9181 tcggtgctga caaagctcat gtagtaaacg aatctatggg agccttaagg aataaactgg
     9241 gtcatgatag aaatctgatt aatcctggat ggcaattact atgggttgta gattggccta
     9301 tgtttgagtt ggatcctcag agcaataagt tacagcctat gcatcatccc tttacatccc
     9361 ctcaggaatt atctgctgag gcacttcgct ccaagccaac gcaaacatta gccaaggcat
     9421 atgatatagt tattaatggt tatgaaattg gcggtggatc tattcgtatc catcaacctg
     9481 aactacaaaa aactgtattt gatttgatcg gaattgatga gcaagaagca catgagaaat
     9541 ttgggttttt actcgatgcc ttacaatatg gtgctccacc tcatggagga attgcgttgg
     9601 gaattgatag attagctatg ctgctgacag actcaacttc cataagggat gtgattgcgt
     9661 ttcccaaaac tcaaacagca tcttgtcctt taacaagtgc gccatcgcct gcgggcaatg
     9721 cacaattaac tgaactgggt ataagacttg cgccaactat tacgacaaag taatttaaat
     9781 ttaagcacca caagttttaa atacttgtgg agctttttcc tgcctctatt tttattatta
     9841 tttagttttt cgctcttttc atcgtcggtt aaagaaaata atgccacgct agtagaatat
     9901 ttggctcatg aaaaaactca tttgaccatt gctatcaatg aaattaagca aaagagcgct
     9961 cccaaggatg agcaagagta caatgagaag ttaagacaaa ttagaactat tctttcaatg
    10021 aataaagcaa aaattgagag tttgaatagt tttagtgaaa atcaaaagaa agaagaagtc
    10081 aatttgaatg accaactaaa gcgtttgcaa caaatgccac ttgccaatgc tgaagtaacc
    10141 attcaggaaa aagtaggtaa aatcgagact ttgcttactg ttaataaaaa aactactgag
    10201 ttaattgcag aaactttgga tttagccaaa cagtatcaat tacttttaaa tgatattttg
    10261 aatgaattaa aactttggaa agccaacttc gatttgaaag aaaagcttaa gtcaattaaa
    10321 gctattagag ctaaattgaa taaagagctg aatatccttt atgagaatgc agtatccagc
    10381 caagatcaaa agcaacagaa attgacgatt agcgaacaga ttcaatatga agctaatttg
    10441 tacatcaata atcaaagaat tgcagttatt catcaaaagt tgaatgcatt ggagttacag
    10501 aaaaaggtaa ttaaaacgaa tatcctatt
//
LOCUS       DASLOE010000106        10526 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.107, whole genome shotgun sequence.
ACCESSION   DASLOE010000106 DASLOE010000000
VERSION     DASLOE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10526
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.107"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..680)
                     /locus_tag="U6309_002047"
     CDS             complement(<1..680)
                     /locus_tag="U6309_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEN5505485.1"
                     /translation="MPNSSNVLRHMENHFALTTCEDIKNIMQPLLIKHGMTVFNYYRI
                     YFDSTVIRLSTDQKWTEHYFKMNYLETLTIPESYLKKPLNYYLWLTDDCPLMLQDAAI
                     NFDMANGISIAKINHDSIEYFCFASTRDNTSIVNNFYLNNLDVLEQYSLYFKDQFNSI
                     ISQFEKNKIILPYNATCCQSNKINKEIKRSLLSPRQKDCAKLLLQGMSYKEIGKILQL
                     SARTVETHV"
     gene            complement(943..1689)
                     /locus_tag="U6309_002048"
     CDS             complement(943..1689)
                     /locus_tag="U6309_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal large subunit pseudouridine synthase
                     B"
                     /protein_id="HEN5505486.1"
                     /translation="MSSERLQKILSQAGLGSRREMERWIDNGWVQVNGKPAKLGDSAG
                     PGDKISVKGKLIPNPLKGKQNIRVLLYHKPVGEVSSRHDPKFEKTVFDNLPHLRQGRW
                     VQVGRLDLNTSGLLIFTNNGELANQLMHPKYGLEREYAVRVHGQVSQESLNNLLKGVM
                     LDDGLAKFTKIQFRGGEGTNSWYHVTLNEGRNREVRRLWESQGVEVSRLIRIRYGMLT
                     MPRFLSRGQFYELTPKEVTEFLDSLPNELS"
     gene            complement(1679..2260)
                     /gene="scpB"
                     /locus_tag="U6309_002049"
     CDS             complement(1679..2260)
                     /gene="scpB"
                     /locus_tag="U6309_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEN5505487.1"
                     /translation="MIDNELKNIVEALVLSNNEPVTLEKIQETFDEWQRPTLEDLQAI
                     IESLKVDYSSRAFELIQVAGGFKVQTKSKYATWVARLQIEKPAKYSRALLETLAIIAY
                     KQPVTRADIEDIRGVAVNSQIIKTLMEREWIRIAGYKDVVGKPAVYTTTKEFLNYFNL
                     NYLSELPPLPEIMDTLTLHEATQPIKECTQNEQ"
     gene            complement(2262..3053)
                     /locus_tag="U6309_002050"
     CDS             complement(2262..3053)
                     /locus_tag="U6309_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEN5505488.1"
                     /translation="MNIDLQPAPVVKAVVDGKEITQFPEDLYIPPDALEVLLDSFTGP
                     LDLLLYLIRKQNIDILDIPMTSITNQYLQYIQLMENRRMELAAEYLLMAAMLAEIKSR
                     LLLPVTPDSEEEEEDPRMTLVRQLQAYEQIKQAAELLDGLPRQDRDNFTIQVNPDEVE
                     TIKVYPEVELSELVEAMKVLLKREEHHSKHQVSRELMSVRERMSRILFQLQHQKILEF
                     SQLYTLEEGRMGLVVSFLAILELAKQSLLIITQTQPFSPIHLQAA"
     gene            complement(3053..4267)
                     /locus_tag="U6309_002051"
     CDS             complement(3053..4267)
                     /locus_tag="U6309_002051"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEN5505489.1"
                     /translation="MSALFTSNKRVVSGMRVSGRLHLGHYHGVLKNWIKLQHQYDCFF
                     FAADWHGLTTQYEDPNFIEKHLWDMIIDWLACGVNPGLCKIFIQSWVPEHAELHLLLS
                     MITPLGWLERVPTYKDQQEKLKEKDLSTYGFLGYPLLQSADVLLYHADYVPVGEDQVA
                     HIELTREIARRFNYLYGREANFEELAAAAIKKMGKKNGKIYTELRRQFQEHGIHESIK
                     TAQALLEDQPNLSIGDKERLLGYLEGSGKIILNEPQPLLTETAKMPGLDGQKMSKSYH
                     NTIMLRDEPALVEKKILTMPTDPARVKRTDPGEPEKCPVWQFHKVYSNEEVKDWVQKG
                     CRTAGIGCIDCKRPVIGAIHQELKPIQEAISDYEADLGSVKRIVAEGSEAAREEGNKT
                     LNTVREVMGLDY"
     gene            complement(4513..5133)
                     /locus_tag="U6309_002052"
     CDS             complement(4513..5133)
                     /locus_tag="U6309_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEN5505490.1"
                     /translation="MSQFFALHPDNPQARLLRKAVSIIEEGGLIVYPTDSGYALGCSL
                     GNKSALERIRRLRQLDKNHNMTLVCRDLSQLGTYAKVSNPIFRLLKAFTPGSYTFILN
                     ATHEVPRLMLHPKRRTLGLRVPDNTITLSLLECLEAPLMSTTLILPGAEAPLSEPEAI
                     KDLLGNQIDLIIDGGNCGHEPTTVVDLTGDYPIIIREGKGDPEPFK"
     gene            complement(5130..5969)
                     /locus_tag="U6309_002053"
     CDS             complement(5130..5969)
                     /locus_tag="U6309_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEN5505491.1"
                     /translation="MIDLHCHSSFSDGALTPEELIKRASTLQVQCLSLTDHDTVAGYD
                     ALYGAAIHTNIEIVKGIELSTRWKKYDIHILGYQINHTTELQELIAHQNKSRIERAEK
                     IGESLHSIGIDKAYEKACLLAGHKRVGRPHFAQVLINEGKAKDMKSAFKNYLGRGKCA
                     YIPTLWISMHDAVKGISHSGGQAVIAHPIKYGLTRSKLRELITEFKEAGGVGMEVVSG
                     EMTTVQIQEMAGMCERFHLLASSGSDYHSDAQSCINLGRQRQLPMICTPIWQEWNMKQ
                     GTL"
     gene            6247..7197
                     /locus_tag="U6309_002054"
     CDS             6247..7197
                     /locus_tag="U6309_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEN5505492.1"
                     /translation="MSSTLNSEIIKLPPLNTRELANLCGVLHSNLKILEQHFKVSIKI
                     KNHSMIICGQSGDHFEEIKKVLKLLYPLTSEPIDTKTLRVLINEDYQTAMTHHIRLSR
                     KSISARNSKQAAYLDSINTHDITFAVGPAGTGKTYLAVSKAIECFEKGEVQRLIFVRP
                     AVEAGEKLGFLPGDLVEKVLPYLRPIYDALYEMIGFKETQKLIQTDIIEILPLAFMRG
                     RTLNESFIILDEAQNTTIMQMKMFLTRMGFGSKTVITGDITQVDLPKGIDSGLAHAIQ
                     LFRHFPDISIHTFTSREVVRHPLVSKIVDCYDNEMERIKK"
     gene            7194..7670
                     /gene="ybeY"
                     /locus_tag="U6309_002055"
     CDS             7194..7670
                     /gene="ybeY"
                     /locus_tag="U6309_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEN5505493.1"
                     /translation="MTYHIDIQNATGKLLPLSEDEITKLASLALRDHKQDAELTVRLV
                     DVEEMTYLNHTYRKKNKPTNVLAFPCSLPANIELECPLLGDVVICPEVLLAESAQFNK
                     SLHAHWSLILIHGVLHLLGYDHIKDEEASIMQMLEAKLLAELGYANPYEVEENELE"
     gene            7630..8520
                     /locus_tag="U6309_002056"
     CDS             7630..8520
                     /locus_tag="U6309_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN5505494.1"
                     /translation="MLIHMKSRKMNLNKLEDGGSWFSRLKQFLQGEPQNQEELVSLLR
                     DAQIRSLINSETLAMIEGAILFSKMRVRDIMLPKNQMVCINKDDDFKHIISIVTQTGH
                     SRFPVTSENSDEVVGILHAKDLLKYQPENMESFDLLDICRQVTFVPESRRLDSLLSEF
                     RSNRNHMAIVVDEYGEVSGFVTIEDIIEQIIGDIEDEFDIDEDAYIKAHEGHCYIIKA
                     HTPIEEFNEQLNADFSDETYDTIGGIVLNKFGYLPQRGEVIIIDDFEFKVLNADSRRI
                     KLLECLDKRSPEKLNSTVEG"
     gene            8528..9208
                     /locus_tag="U6309_002057"
     CDS             8528..9208
                     /locus_tag="U6309_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN5505495.1"
                     /translation="MSSSILIIDDDTELTDLLTQYLEPEGFNVVCVHDGENGVKKALN
                     QVFDAIILDVMLPKLNGFEVLKAIREHLETPVLMLTARGDDIDRIVGLEIGADDYLPK
                     PCNPRELVARLRAILRRTQKIPTPKPIIEQHNIIVDCSKRHVTMGGKFLELTNAEFNI
                     LEMLIKSPGQAFSKEELTEYALGRKYTAYDRSIDVHISNLRNKLGDNPQGEPLVKTVR
                     GFGYMFNA"
     gene            9201..>10526
                     /locus_tag="U6309_002058"
     CDS             9201..>10526
                     /locus_tag="U6309_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEN5505496.1"
                     /translation="MRSLYWKIFVSFWLATILIIFTTAWVTSQIAQKSSQPAREEMFM
                     DSYANAAVATYESGQQSALLKWLDKIGLSRHMTLYLLTSTGEIIGAQKPPDAVKKISE
                     NLINDELPSDGIFKTGKLVVSHEILSTSGKYYRLAAVSEKPISYFVQIPWAGLTIRLT
                     LAIFISGLICYLLSRYLTQPLRSLGMAAKSIAKGNLNTRVGRFRGHSKDEIAELSNEF
                     DRMAEQLEALVHSKERLIQDISHELRSPLARLKIAIELGKKKTKHLADNEFARMEIET
                     SRLNDLIGEILDFARLEKSTTELHLSQTNIADLLAQVISDANYEFSHNSIRIQAGIIT
                     PCELNIDQRLIHRAIENIVRNASHYSPPDEKVLISSSYNKNKDQVYIDINDKGPGVPE
                     DQLEKIFNPFYRVDTSRTKKTGGYGLGLAIAARAVALHQGEIIAKNREQG"
BASE COUNT         3303 a         2092 c         1869 g         3262 t
ORIGIN      
        1 acgtgggttt caacagttct agctgataat tggagtattt tcccaatttc tttataactc
       61 attccttgca ataagagttt tgcacagtcc ttttgacgag gagaaagaag ggatcttttt
      121 atttctttat tgattttgtt gctctgacaa caagtagcat tataggggag tattatttta
      181 tttttttcga attgacttat tattgaatta aattgatctt taaagtaaag actatattgt
      241 tctagaacat ccagattatt gagataaaag ttattaacaa tacttgtatt atcacgtgta
      301 cttgcaaaac aaaaatattc tatagaatca tggtttattt tagcaataga tataccattg
      361 gccatgtcga aatttattgc tgcatcttgg agcatcaaag gacagtcatc cgttagccat
      421 aaataataat tcagcggttt ttttaaatag gattctggaa tagtcagcgt ctccagatag
      481 ttcattttaa aataatgctc agtccatttt tggtctgtgg agagtctaat gacagtacta
      541 tcaaagtaaa ttcgataata attaaagact gtcataccat gttttatgag caaaggttgc
      601 atgatatttt ttatatcttc acaggtagtc aaggcaaagt gattttccat atgccttaga
      661 acatttgatg aattaggcat tagttctctc ttcaaactca tgggcaaata atcttgcgta
      721 gattttcaga tctggattca gcattttttg gaaagccatg taaattgctc cagtgcttct
      781 tcagatgtta aattttcgtt ccctaaaact tcttcaactt tgaatatgtt tatacatgtg
      841 aggataggat taatcaaaaa taactgtcca gaaagtcatc attcttttca agacagctaa
      901 catagtgaat acaatgaaat ccaacgtctg attttaaacc atttaagaca attcattggg
      961 taatgaatca agaaattcag tcacttcttt gggggtaagt tcataaaact gccctctgga
     1021 taaaaatctg ggcattgtaa gcatgccata acggattctt attaatcgac taacctcaac
     1081 accttgagac tcccacaacc gacgtacttc tctatttctt ccttcattaa gggtcacatg
     1141 gtaccaggaa tttgttcctt ctcctccacg aaattgaatt ttggtaaatt ttgccaaacc
     1201 atcatccaac ataacccctt tcaataagtt atttaaagat tcctggctga cttgaccatg
     1261 aactcgtaca gcatactctc tttccaaacc atatttggga tgcatgagtt ggttagccaa
     1321 ttcgccatta ttagtaaaaa tgagcaaacc ggaagtattt aaatccagcc gccccacttg
     1381 cacccaacgt ccctgtctaa gatgcggtaa attatcaaat actgtttttt caaattttgg
     1441 atcatgtcga ctggatactt caccgacggg tttatggtaa agaagaaccc ggatgttttg
     1501 tttgcctttt aaagggttgg gaatcaactt tcctttgacg ctaattttat caccagggcc
     1561 agcggaatct cccagtttag ctggtttgcc attaacctgc acccaaccat tatcaatcca
     1621 ccgctccatt tcacggcgag atccaagtcc ggcttgactt aatattttct gtaatctttc
     1681 actgctcatt ttgcgtacac tcttttatag gttgggttgc ctcgtgcagg gttaatgtat
     1741 ccattatttc aggtaaggga ggaagttcac ttaagtaatt taagttaaag taatttaaaa
     1801 attcttttgt cgttgtataa acagccggct tccctactac atctttatag cctgctatac
     1861 gaatccattc cctctccatt aacgttttta taatctggct gttaacggca actcctctaa
     1921 tgtcttcaat atcagcgcgc gtaacaggtt gcttgtaagc aattattgcc aaagtttcaa
     1981 gcagtgcgcg cgaatatttt gctggcttct cgatttggag acgagcaacc caagtggcat
     2041 atttggattt tgtttgtacc ttaaaaccac cagcaacctg gataagctca aaagcacggg
     2101 aagaataatc tacctttaag ctctctatga tagcctgcaa atcttctaat gttggtcttt
     2161 gccactcatc aaaggtttct tgtatttttt ctaaagtaac gggctcatta ttggataata
     2221 ccaaagcttc aacaatattt tttaattcgt tgtcaatcat ttcatgcagc ctgcaaatgt
     2281 ataggtgaaa atggctgagt ttgcgtaata atcaataaag actgtttagc cagttcaaga
     2341 atagctaaaa aactcacaac caatcccatc cttccctctt caagagtata taattggcta
     2401 aattccaaaa ttttttgatg ctgcaattga aagagaatac gactcattcg ttcacgaaca
     2461 gacattaact ccctggaaac ttgatgtttg gaatgatgct cttcacgttt cagcaaaact
     2521 ttcatagcct cgactaattc agataactca acttcaggat aaactttaat tgtttcaacc
     2581 tcatctggat ttacctgaat tgtaaaatta tctctgtctt gtcttggcaa tccatccaat
     2641 aattcagccg cttgttttat ttgctcataa gcttgcaact gtcttaccag agtcatgcgt
     2701 ggatcttcct cctcctcctc tgaatctggt gtgactggca ataataaacg tgacttgatt
     2761 tcagctaaca tggcagccat taataaatac tcggcagcca gctccattct tcgattttcc
     2821 atgagctgga tatattgtaa atactggttg gttatacttg tcatgggaat atcaagaata
     2881 tcaatatttt gcttgcggat taaatacagc aataaatcta atggcccggt aaaagaatcc
     2941 agtaacacct ccaatgcatc aggaggaata tacaaatcct ctggaaactg agtaatttcc
     3001 ttaccatcca ccactgcttt gactacaggc gcaggctgca aatcgatatt cattaataat
     3061 caagccccat gacttctctg actgtattta aagtcttgtt tccttcttct ctggccgctt
     3121 cacttccttc tgctacaata cgttttaccg aacctaaatc agcttcataa tctgaaattg
     3181 cttcctgtat tggtttaagt tcttgatgga tagcaccaat cactggcctc ttacagtcaa
     3241 tacaaccaat tcctgctgtt cggcaacctt tttgcaccca atcttttact tcctcattag
     3301 aatacacttt atggaattgc caaacaggac atttttcagg ctctcctgga tctgtacgtt
     3361 taacacgcgc cggatccgtg ggcatagtca gaattttctt ttcaactaaa gctggctcat
     3421 ctcttagcat gatcgtatta tggtaagatt tggacatttt ctgcccatca agaccaggca
     3481 tttttgcggt ttccgtaagc aaaggctgtg gttcgtttaa tataattttg ccacttcctt
     3541 ccaaatatcc caacaaccgc tctttatctc caatcgacaa attaggctga tcttctaaaa
     3601 gcgcttgtgc tgtcttaatc gattcgtgga tgccatgctc ctgaaattgt ctgcgtaatt
     3661 cggtataaat tttcccattc tttttaccca tctttttgat tgctgcagca gccagctcct
     3721 caaaattagc ctctctacca taaagatagt taaatcttcg ggctatttct cgtgttaact
     3781 caatatgagc cacttggtct tcacccactg gaacataatc cgcgtggtat aaaagtacat
     3841 cagcactttg tagtaaagga tatcccaaaa aaccataagt tgataaatct ttttctttta
     3901 atttctcttg ctgatcttta taagtaggaa ctcgctccaa ccaacctaaa ggagtgatca
     3961 tggacaaaag taaatgtaat tctgcatgct ctggaaccca ggattgaata aatatcttac
     4021 ataagcctgg attaactcca caagctagcc aatcaataat catatcccaa agatgttttt
     4081 caataaaatt ggggtcttca tattgtgttg ttaggccatg ccaatctgct gcaaaaaaga
     4141 aacaatcgta ttgatgttgt aattttatcc aattttttaa aacaccgtga taatgaccta
     4201 aatgcaatct tccactgacc cgcattccag aaacgactcg cttattggaa gtaaacagtg
     4261 ctgacatcaa tttcctcttc ttgttataaa ttaatcaatt atttcaaata caaaaatttc
     4321 tttattccct gaattctaac ctgatgcttg cttcaatcat attttatcca aattttatca
     4381 agattagaga tatgagatta ataatcaaaa gctaactgga caaaataaaa ttcaatggcg
     4441 tcagctctag tctggtagga tcacataaaa ttctctttca ctaaatacta acaaaaccgc
     4501 gactgtattc cattacttaa agggttccgg atctcctttt ccctctctta taattatcgg
     4561 ataatctcca gttaaatcga ctactgtagt gggttcatgg ccacaatttc caccatcaat
     4621 tattaaatca atttggttgc ctaataaatc tttaattgct tcgggctcgc ttaatggcgc
     4681 ttcagcccct ggtaaaatta aagtggtgct cattaaaggg gcttccagac actccaataa
     4741 agacaaagta atcgtgttat caggtactct taaccccaat gttcttcgtt ttgggtggag
     4801 catcaaacga ggcacttcat gtgtagcatt aagaataaag gtataagagc ctggagtaaa
     4861 agccttcaat aatctaaaaa ttggattaga aacctttgca taagtaccta actgggataa
     4921 gtcacgacaa accagggtca tattatgatt cttatccaat tgacgtaagc ggcgaatacg
     4981 ctccagggct gatttattgc ccaaactaca gcccaaagca tagccagaat ctgtcgggta
     5041 aacgataagc cccccttctt caataattga aactgctttt ctcaacaagc gtgcttgagg
     5101 attatctgga tgtaaagcaa aaaactgact cataaagtcc cctgtttcat gttccattct
     5161 tgccaaatag gcgtgcaaat cattggtaat tgtcgctgac gccccagatt tatacaagat
     5221 tgagcgtcac tatggtaatc tgaacctgat gaggcgagca aatgaaatcg ctcacacatc
     5281 ccggccattt cttgaatttg tacagttgtc atttctccag aaacgacttc cataccaaca
     5341 cctccagcct ctttaaattc cgtgattaac tcacgcaatt ttgaacgcgt caatccatat
     5401 ttaattggat gagcaattac agcctgtcca ccagaatgag atataccttt gaccgcatcg
     5461 tgcatgctaa tccataaggt aggtatatag gcgcacttgc ctctcccgag gtaattttta
     5521 aaagccgact tcatatcttt tgccttccct tcattaatca gtacttgggc aaaatggggt
     5581 cgacccaccc gtttgtggcc tgccaaaaga caggcttttt cataagcttt gtcaatacca
     5641 atcgaatgca aggattctcc aattttctcg gcgcgctcta ttcgactttt attttgatga
     5701 gcaattaact cttgtaattc tgttgtgtgg ttaatttggt aaccaagtat atgaatgtca
     5761 tacttcttcc aacgagtact gagttctatc cctttaacta tttcaatatt tgtatggatt
     5821 gcagcaccat ataaagcgtc atatcctgca acagtatcat gatcagtaag agataggcat
     5881 tgaacttgta aagtactcgc tctttttatt agttcttctg gagtcagtgc accgtctgaa
     5941 aaagaactat gacaatgaag atcgatcata aattgcagct aatttaaaaa aatcgaagta
     6001 tagcataatt aacgtgagtt cgacataaaa ggcttttatg acttttatgt cgctaaacgc
     6061 acgttatccc gtaaaaaagc aaagcttttt atacgggatc cattcaaaat acctaattct
     6121 atataagaaa aaccaggtaa aaatatgatg ttgtaaagac taaactatcc ttttctttga
     6181 acaacttgca caacatagta cttttgcact attctttaag tatggtcatt aatctttaat
     6241 aaaactttga gtagtacatt aaacagtgaa ataatcaaat taccacctct taataccagg
     6301 gaactcgcaa acctgtgtgg ggttttgcac agcaacttaa aaattttaga acagcacttt
     6361 aaggtttcaa taaagatcaa aaatcatagc atgataatat gtggccaatc aggtgaccat
     6421 tttgaagaga taaaaaaagt attaaaatta ttgtatcctt taactagcga acctatagat
     6481 accaaaactc tacgagtctt aatcaatgag gattatcaaa cagcaatgac tcaccatatc
     6541 agattatctc gaaaatcaat ttctgcacgt aacagcaaac aagccgcata tctggattct
     6601 attaatacgc atgatattac atttgccgta gggccggctg ggactggtaa aacctacctg
     6661 gctgtttcca aagcaataga atgttttgaa aaaggcgaag tacaaagact tatttttgta
     6721 aggcctgctg tagaagcagg tgaaaaactc ggttttcttc ccggcgatct ggtagaaaaa
     6781 gtcttacctt atttaaggcc tatctatgac gcactgtatg aaatgatagg ctttaaagaa
     6841 actcaaaagc tgattcaaac agatatcatt gaaattctcc cattagcttt catgaggggt
     6901 agaactttaa atgaatcttt tattattctg gatgaggctc aaaacaccac catcatgcaa
     6961 atgaaaatgt ttttgacacg catgggcttt ggctctaaaa cagttatcac aggtgatatc
     7021 actcaagtcg acttgcctaa aggtattgac tcgggccttg cacatgccat tcagttattc
     7081 agacattttc ctgatatcag cattcataca tttaccagtc gtgaagtcgt cagacatcct
     7141 ttggtttcaa aaatcgtaga ttgctatgat aatgagatgg aaaggattaa aaaatgacct
     7201 atcacattga catacaaaat gctacaggga agttattgcc cttaagtgaa gatgaaatca
     7261 ccaagctggc atcactagct ctaagagatc ataagcagga tgctgaacta acagttcgtc
     7321 tggttgatgt tgaagaaatg acttatctta accataccta tagaaagaag aacaaaccaa
     7381 ccaatgttct tgcatttcct tgctcactac cggcaaacat tgaattggaa tgtcctcttt
     7441 tgggtgacgt cgttatttgc cctgaagtgt tgcttgcaga aagtgctcaa ttcaataaat
     7501 cgctacatgc acattggtct ttaatcctga ttcatggagt gttgcattta ttaggttatg
     7561 atcatataaa agatgaagaa gcctctatta tgcaaatgct cgaagctaag ctactagctg
     7621 aattaggtta tgctaatcca tatgaagtcg aggaaaatga acttgaataa attggaagat
     7681 ggtgggtcgt ggttttctcg tttaaaacag ttcttacaag gagagccaca aaatcaggaa
     7741 gaattggtca gtttattaag agacgcacaa atacgctccc tgattaattc agagaccctc
     7801 gcaatgattg agggagctat ccttttctca aaaatgcgtg ttagagacat aatgctgcct
     7861 aaaaatcaaa tggtttgcat taataaggat gatgatttta agcatattat atctattgta
     7921 actcaaacgg gacattcacg ttttcctgta acgagtgaaa attctgatga agtggttggc
     7981 attttgcatg caaaagactt gttaaaatat caacctgaaa atatggaatc atttgattta
     8041 ttggacatct gccgccaggt aacttttgta cctgaaagca gacgtcttga tagtctgctt
     8101 agtgaatttc gaagtaatag aaatcatatg gctattgttg ttgatgaata tggagaggtg
     8161 tcaggtttcg ttaccatcga agatatcatt gaacaaataa tcggtgacat agaagatgaa
     8221 tttgatatcg atgaagacgc ctatatcaaa gcacatgaag gacactgcta tatcatcaaa
     8281 gcccatactc caatagaaga atttaacgaa cagctcaacg ctgattttag tgacgaaacc
     8341 tatgatacaa ttggtggcat agtattaaat aaattcggct atttacctca acgaggtgaa
     8401 gtaataatca tagatgattt tgaatttaaa gttcttaatg ccgattcgag aagaataaag
     8461 ttattagaat gtctcgataa aagaagtccc gagaaactaa attctactgt tgaaggataa
     8521 gtaattcatg agcagctcta ttctcattat tgatgatgac acagaactaa cagatttatt
     8581 aacccaatat ttagagccag aaggctttaa tgtagtatgc gttcatgatg gagaaaatgg
     8641 tgttaaaaaa gctttaaatc aggtatttga tgccattata ctcgacgtca tgctgcctaa
     8701 gcttaatggt tttgaagtat taaaagcaat aagagagcat ttggaaacac ctgtattgat
     8761 gttaactgct cgcggtgatg atatcgatcg cattgttggg ttagaaattg gtgcagatga
     8821 ttacttacct aaaccctgta atccaagaga gttagttgca cgtctgcgtg ctattttaag
     8881 acgcactcaa aaaatcccaa ctcccaaacc aattattgaa caacataata tcattgtgga
     8941 ttgctccaaa cgccacgtga ctatgggagg aaaattcctt gaattaacca atgcggaatt
     9001 taatattttg gagatgttaa tcaaatcacc aggacaagca ttttccaaag aggaactcac
     9061 cgaatatgct ctgggaagaa aatacactgc ttatgaccgc agtatcgatg ttcacatcag
     9121 caatttgcga aataaattag gcgataatcc ccagggggaa cctttagtca aaactgtccg
     9181 cgggtttggg tacatgttta atgcgtagcc tgtactggaa aatatttgtt tctttttggc
     9241 tggccaccat tttaattatt tttactacgg catgggttac cagccaaatc gcacaaaaat
     9301 catcacaacc agctcgagaa gaaatgttca tggacagcta tgccaatgca gctgtcgcaa
     9361 cctatgagtc aggccaacaa tcagctcttt tgaaatggct tgataaaata ggcctttctc
     9421 gtcatatgac actgtattta ctaaccagca ccggtgaaat cattggcgca cagaaaccac
     9481 ctgacgccgt aaaaaaaatt tcagaaaatt taataaatga tgaactccca agtgatggca
     9541 tctttaaaac agggaaatta gtcgttagcc atgagatcct atcaacttct ggaaaatatt
     9601 accggctagc agcggtcagt gaaaaaccaa tctcttattt tgtgcaaatt ccatgggctg
     9661 gactgacaat acgcttaact ctggccatat tcatcagtgg gcttatctgc tatttactct
     9721 cacgttattt aactcaacct ttacgctcat taggcatggc agcaaaatct atcgctaaag
     9781 gaaatttaaa tacccgggtt ggtcgcttca gaggtcatag taaagatgag attgcggagt
     9841 taagtaatga attcgatcga atggccgagc aattagaagc gcttgttcat tccaaagaac
     9901 gattaataca ggatatttcc cacgaattac gctctccatt ggctcgactt aaaatcgcca
     9961 tagaattggg gaaaaagaaa accaagcatt tggctgataa tgaatttgcc cgcatggaaa
    10021 ttgaaacatc cagacttaat gatttgatag gcgaaattct cgattttgct cgtttagaaa
    10081 aatcgacaac agaacttcat ttatcccaaa caaatatcgc agatttgctt gcacaggtta
    10141 taagtgatgc caattatgag ttcagtcaca attctataag aatacaagct ggaataatta
    10201 caccttgtga attaaatatt gaccaacgcc taatacacag agctatagaa aatattgttc
    10261 gtaatgcatc acactattct cctccagatg aaaaggtcct tatttcatca agctataata
    10321 aaaacaaaga tcaagtttat attgatatta atgataaagg acctggggtg cccgaagatc
    10381 aactggaaaa aatatttaat cctttctacc gcgtggatac ctccagaaca aaaaaaaccg
    10441 gtggctatgg ccttgggctc gcaattgcag ccagggctgt agcattacac caaggagaaa
    10501 ttattgctaa aaatagagag caaggt
//
LOCUS       DASLOE010000107        10487 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.108, whole genome shotgun sequence.
ACCESSION   DASLOE010000107 DASLOE010000000
VERSION     DASLOE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10487
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.108"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..438
                     /locus_tag="U6309_002059"
     CDS             <1..438
                     /locus_tag="U6309_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505497.1"
                     /translation="RVNILFSRFLKSLDEQQRRKLILTLSLFDPVEAKSKFLTNCIHY
                     VTNRLCQISMHQLDSSPNFFGTYQCIDSKKLLISKTDVKQVSAILEAFKEMLHSLEER
                     CNPEQLENMLIFLRNISRPILTVAEVEEAQQSARVIDYIGAPT"
     gene            599..1768
                     /locus_tag="U6309_002060"
     CDS             599..1768
                     /locus_tag="U6309_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2744 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505498.1"
                     /translation="MSNLIELALRNAALDRTKLLSQVQKIEFLNGYDPRFVEGLISLE
                     MIDDIDEYVASLMYVKMTDKYTQEEKEQLTDTILTINNDRLISCQYRFPQLYQKLMQD
                     QFLSSFFSSSHLLPNIAPGTNNQSYVTVSTNNKMKMESAKLYGHYEYYQCVVALITQI
                     DGSRYFYHLNPNRIFGDYMGSAPLKLSGSELKDVKNITFILHPRSMVDYRELQRLQER
                     NIVFKTVMLPVGAKSVSLSYDTDKDVLTVNNLEAGQTVTYNNLLQTPQYNRYQYTPEQ
                     YFMTQLYRGQTWAEALGEALNKYIVTRQEGATWWDLKPLFFNRETTKNNMIAAARIKK
                     EVEAGATSFDFTGVSRNGLLNQLLTTAVDYQLEYQRGLQNGMLSAEDLTTSAALR"
     gene            complement(1803..2780)
                     /gene="rsgA"
                     /locus_tag="U6309_002061"
     CDS             complement(1803..2780)
                     /gene="rsgA"
                     /locus_tag="U6309_002061"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HEN5505499.1"
                     /translation="MSKRRINKQQSARIAKIQKNYHESMENNSDNLADGLVITRFSRH
                     AEIEDNQGKRILCSIRPNLDTLVAGDRVIWQIEGEKQGVVVSIYPRGSLLARPSQKGL
                     KKPVAANITQLVVVIAPKPEISWPLLDSYLIMAEILQLHVVIVLNKIDLPCQTLQQRL
                     MTDYQLLNYPVIMASNQTPDTIVPLKQALNNQISVFVGQSGVGKSSLISSLLPHEENI
                     AIDKISMVSELGKHTTRNSRYYHLPSGGALIDSPGVREFSLWDVDCSMIAQGYKEFKP
                     YLSQCKFRNCTHIDTPQCAIIDALNKGKISTQRYENFTKLCAQFNSLKE"
     gene            2843..4075
                     /locus_tag="U6309_002062"
     CDS             2843..4075
                     /locus_tag="U6309_002062"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEN5505500.1"
                     /translation="MKVYLVGGAVRDRLLGIPVQEQDWVVVGATPEELLKRKYRQVGR
                     DFPVFLHPETKEEYALARTERKSAPGYYGFICDFSESVTLEEDLARRDLTINAMAMDE
                     QGNLIDPYQGQRDLEEKLLRHVSPAFAEDPVRVLRVARFASRFHHLGFRIANETRLLM
                     YSMVKQGELAHLIPERVWQEWQKSLEEKNPEQFILSLRSCDALRVILPEINSLFGVPN
                     PYQYHQEIDTGIHSLMALRASSELSEEPLVRFAALVHDLGKASTPIQAWPKHHGHEEE
                     GTKLIRALCTRLRIPNDYRDLAVTVARAHLNIHRVCELRPNTVVKLLEQVDAFRRPQL
                     FHKILIVCQADAESCGKTVVYRQTQLWNEILSECVKVKPQTFIVQGYEGKAIKEAMHQ
                     SRVACVERIMTSWKSNEK"
     gene            4065..4628
                     /gene="orn"
                     /locus_tag="U6309_002063"
     CDS             4065..4628
                     /gene="orn"
                     /locus_tag="U6309_002063"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEN5505501.1"
                     /translation="MKNNQNLIWIDLEMTGLEPEQDRIIEMATIVTDPQLNILAEGPV
                     IAVSQPKILLDSMDAWNTKQHNQSGLVKRVLESNVSESQAEQLTIEFLKQYVDKGKSP
                     MCGNSICQDRRFLYKYMPELAAYFHYRNLDVSSLKELVLRWRPELMNGVVKESKHLAL
                     DDIKDSINELIYYRQHFINLPEVKNDK"
     gene            4618..5280
                     /locus_tag="U6309_002064"
     CDS             4618..5280
                     /locus_tag="U6309_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEN5505502.1"
                     /translation="MTNRERLELPNGAKKLLLHSCCAPCSGEVMEALIFSEIDFTIFF
                     YNPNIHPLQEYEIRKEENIKFAQKHSIPFIDADYDKENWFARAKGLEWEPERGKRCTM
                     CFDMRFERTALYAYEHDFPVISSSLGISRWKDMNQINDCGVRAASHYPDLIYWTYNWR
                     KNGGSERMYEIAKREAFYKQEYCGCVYSLRDTNAWRKQNTRDRIKIGLNYYSESKNEG
                     EL"
     gene            5277..6200
                     /locus_tag="U6309_002065"
     CDS             5277..6200
                     /locus_tag="U6309_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN5505503.1"
                     /translation="MSKHSHDGAHHHEHGEVTYDRAFIIAITANGLFVVMQIIFAYLA
                     NSTSLLADAFHNLGDVLGLILAWVATGLMKRKPTHKATYGFKKTSILAALANGALLVF
                     TCGIIATDAMYKLFSPTEIKAVSVMIVAAIGIVVNFSTALLFLRGSDDLNIRGAYLHL
                     FYDALISVGVVLSAGVLYLTGWLWIDPVVGLLIALVILKGTWSLFADSFRLIIDGVPR
                     DISWIKVSDFLLAIPGVKSIHDLHIWAISTKENALSVHLFMPEESLSDDLRRELVEKL
                     KHDFSIQHVTIQVEKTEAECNDACHNPRIII"
     gene            complement(6336..7523)
                     /locus_tag="U6309_002066"
     CDS             complement(6336..7523)
                     /locus_tag="U6309_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEN5505504.1"
                     /translation="MIRVLFAFIILLLSVFLGIQLNKDPGYVLIAINNWTIETTLWVS
                     VFGLFFLFFLIYGLVQLCNKITRMPGMVNKWHSRRLAQKAQATTRKGLIEYSEGYWQK
                     AKNHLIQALPNTDTPLLNYLTAARAAQKMGDSKLRDHYLREAQQSMPEAKIAVELTQA
                     QLQLANHQWEQALATLKHLQDLAPRHPYVLKLLMNLYEEIKDWQQLIALLPDLKKNHI
                     ISDQAFEQLQKNTYLQAMIDLAKHNQSDGITKLYQTLPKTLVNDTAITTEYARFLIRN
                     QDYDQAEYHLRRCLRKELDNRLIELYSLLPCEENQLLFAESLLKKNPHSAALYLCLGR
                     MCLSRHLWGKAKHYLEQSIELNPTPVAFIELGKLHEKLNDPLSSGSCYKKGLELITKD
                     ELS"
     gene            complement(7526..8650)
                     /locus_tag="U6309_002067"
     CDS             complement(7526..8650)
                     /locus_tag="U6309_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEN5505505.1"
                     /translation="MADSNEEQKKTIQPQQSSKVEPDINQQMKAPFFVSKYLSLIISL
                     TSLLALLAIIISIYSIQLDKQFQNHQLVENKKLTDELGRIKAEQNTVQKLLDNNANHL
                     QQIQSDLTSKMDSLNKELQTAMKQKLYQNNDWLLLKARYYLELAQINVHWSDNFNTSV
                     ALLQQADALLKGMNIPKIFTIRQTIAKEIVQLKSISVVDITGILSQLDAAQAAINNLS
                     IQSVVDQQEVLNHITKSEESGKTGWRNRLQDSVNFLEKLVVIRRHNENIQPLISPLYE
                     SAIKENIRLNLQEAQWAILNNNPAVYQFALNQAIANLKRVFNESSQNTDALIKQLSTL
                     QNVKLTQEKPVVGQAIPLINQMIDNKELLVDQANSKKGEN"
     gene            complement(8654..9406)
                     /locus_tag="U6309_002068"
     CDS             complement(8654..9406)
                     /locus_tag="U6309_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEN5505506.1"
                     /translation="MTKSLNGLRILNTRPREQAEVLNKKIIESGGIPIDCPTMEIVAS
                     ETNWINKLPDLTSVNQAIFVSGNAVRYFMMELASKNINWPNSIRVAAIGKGTSRVLNE
                     FNIQVSDVPELPDSEHLLTLNSLQQIRNQTILLVKGEGGRQLIEEYLMLKGAKLCLLS
                     VYKRVMPTIDQEFLNSLWRDDLADIILLTSEQSIHNLFKMFSIEGQMWLQNKPCLVIS
                     DRLAKVASLFGIKKILISHPERMIGTLFDYKD"
     gene            complement(9403..10332)
                     /gene="hemC"
                     /locus_tag="U6309_002069"
     CDS             complement(9403..10332)
                     /gene="hemC"
                     /locus_tag="U6309_002069"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027223811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEN5505507.1"
                     /translation="MSAKIIRIATRQSPLALWQANHVREMLVKQWPNLSIELLPMITS
                     GDRFLKDKLLSAGGKGLFVKELEEALLDKRADLAVHSTKDMPAQLPDGLSLAAICKRD
                     NPFDALISPQFKSLDALPKNAIIGTSSLRRQSQLLAYNPNLQIKTLRGNIHTRLSKLE
                     SGEYQAIILAAAGLERMGLAHNITQLIPDDIMLPTCAQGALCIECRTDDVEIQELIHG
                     LNDPISALCVHTERRVNAKLGGNCHIPFAVYCTITEENLLLLRAKVLNMDGSQMIEDE
                     QKGKIAEAEIIADRCTESLMTKGAMTLLSTIPS"
BASE COUNT         3080 a         1858 c         2164 g         3385 t
ORIGIN      
        1 agagtcaata ttttattttc cagattcttg aagagcttag atgagcaaca gagacgaaaa
       61 ttaattttaa ctttgtcatt gtttgatcct gtagaggcta aaagtaaatt tttaacgaac
      121 tgcattcatt atgtgacaaa ccgactatgt caaattagta tgcatcagtt agacagctcc
      181 cctaattttt ttggcactta ccaatgcata gatagtaaga aattgttaat tagtaaaaca
      241 gatgtaaagc aagtgagtgc tattttggaa gcctttaaag aaatgcttca ttcgttagaa
      301 gagcgttgta accctgagca attggaaaat atgctcattt tcctgcgaaa tataagccgc
      361 cctattttga ctgttgcgga agtggaagag gcgcaacaat cagcccgagt tatagattat
      421 ataggcgctc cgacctgact taattaactg gaattatcct aatacattat tgtgcatttt
      481 gatctcatcg cgttgataag gatcgattgt attggcctag atgagtaaag cttgctccag
      541 tgtgcgaatt gaattataat tagacaattt gtctcattta tgcttttaaa attataatat
      601 gtcaaatcta atagaacttg ctttaagaaa cgcggcactt gatagaacaa aacttttaag
      661 tcaggtacaa aaaattgaat ttttaaatgg ttatgatccc aggtttgtcg agggattgat
      721 atcattagaa atgatagacg atattgatga atacgttgct tctttgatgt atgttaaaat
      781 gactgataaa tacactcagg aggaaaaaga gcaattaaca gatactatat taaccatcaa
      841 taatgatcga ttgatttcat gtcaatatcg ctttcctcaa ttatatcaaa aattaatgca
      901 agaccaattt ttatctagtt tcttttcaag ttcgcattta ctccccaata ttgctccagg
      961 cacaaataac caatcttatg taacagtaag tactaataat aaaatgaaga tggagtcagc
     1021 aaagttatat gggcattatg aatattatca atgtgttgtc gctttaatca ctcaaattga
     1081 tggttctcga tatttttatc atttgaatcc aaaccgtatc tttggtgatt acatgggtag
     1141 tgcgcctttg aaattatcag gtagtgaact taaagatgta aagaatataa cctttatcct
     1201 tcacccgcga agtatggttg attatcgcga attgcaaaga ttgcaagaga gaaatattgt
     1261 ttttaaaacc gttatgctac cagtgggggc aaagtcagta tcacttagtt atgatacaga
     1321 taaagatgta ttgacggtta acaatcttga ggcaggacaa actgtaacat ataacaatct
     1381 gctacaaacc ccccaatata atagatatca atatacacct gagcaatatt tcatgaccca
     1441 actatataga ggccaaacat gggcagaagc acttggcgag gcattgaata aatatattgt
     1501 tacaagacaa gaaggggcaa cctggtggga tcttaaacca ttatttttta atcgtgagac
     1561 tacaaaaaat aatatgattg ctgctgcgcg aatcaaaaag gaagttgagg ctggtgctac
     1621 ctcatttgat tttacagggg ttagcaggaa tggcttgctg aatcaattat taacaactgc
     1681 tgttgactat caattagaat accagcgtgg attgcaaaac gggatgcttt cggctgaaga
     1741 cctaacaaca agtgctgcgt tacgttgata actgattatc tattttcgga agagcttttt
     1801 gtttactctt ttaaactatt aaattgggca cataattttg taaaattttc atatcgttgg
     1861 gttgaaatct ttcccttatt tagcgcgtca atgattgcgc attggggcgt atcaatatgt
     1921 gtgcagttgc ggaatttaca ttgggacaaa taaggtttga attctttata tccttgagca
     1981 atcatagagc aatcaacatc ccacaaactg aattcccgaa cgcctggcga gtctatcaag
     2041 gcaccaccac taggtagatg ataatagcga gagtttcttg tagtatgttt gcctaactct
     2101 gaaaccatgg atatcttatc gatcgcaata ttttcctcat gtggcaataa acttgaaatc
     2161 aatgatgatt tgcccacccc tgattggcca acaaaaacac tgatttgatt atttaatgct
     2221 tgttttaatg gtactattgt gtctggggtt tggttacttg ccatgatgac aggatagttc
     2281 agcaattggt aatccgtcat gagtcgctgc tgaagtgttt gacagggaag atcaatttta
     2341 ttgagaacga tcaccacgtg taattgaagt atctctgcca taatcaaata gctgtcaagt
     2401 aatggccaac taatctctgg ttttggagca atcactacaa ccagttgagt gatatttgcc
     2461 gcaacgggtt tctttaaccc cttctgactg ggccttgcca gtaagcttcc cctgggatag
     2521 atgctcacaa ccaccccttg tttctcacct tctatctgcc agattactct atcaccagct
     2581 accagtgtgt caagattagg ccggatagag caaagaattc gtttcccctg attgtcttca
     2641 atttctgcat gcctgctaaa tcgagtaatg actaaaccat cagccaggtt atcggagtta
     2701 ttttccatgc tttcatggta gtttttctgg attttagcta tacgggcaga ttgctgctta
     2761 ttgattcgtc ttttactcat agtgtcaatc tatactataa ttgatgcgat taataataca
     2821 gtatgaaggt aaatcatatt taatgaaagt ctacttggta ggtggtgcag ttcgtgatcg
     2881 attattagga attcctgtcc aggaacagga ttgggtcgtc gtcggagcaa cgccggaaga
     2941 attattaaaa agaaaatata ggcaagttgg gcgggatttt cccgtttttt tgcatcctga
     3001 aacgaaagag gaatatgcat tagcaagaac agagcgtaaa tcagctccag gatattatgg
     3061 attcatttgt gattttagtg aatcagtgac gttggaagag gatttggcac gacgagattt
     3121 aacgattaat gccatggcta tggatgaaca gggaaattta attgatccat atcagggaca
     3181 gcgagatctc gaagagaaat tattacgtca tgtgtctccc gcttttgcag aagatcctgt
     3241 tcgggtttta cgtgttgccc gttttgccag tcgatttcat catcttggat ttagaatagc
     3301 caatgagact cgtttattaa tgtattccat ggttaaacaa ggtgaattag ctcatttgat
     3361 ccccgagcgt gtttggcagg aatggcaaaa aagtcttgaa gaaaaaaatc ctgaacaatt
     3421 cattctttct ttacgttcct gtgatgcatt aagagtgatt ttgccagaaa ttaactcatt
     3481 atttggtgtg cctaatccgt atcaatacca tcaagaaata gatacgggca ttcattcatt
     3541 aatggcatta cgggcatcat ctgagcttag cgaagaacca ttagtacgat ttgctgcttt
     3601 ggttcatgat ttaggcaaag cctcgacacc aattcaggct tggcctaaac accatgggca
     3661 tgaggaggag ggtaccaagc tgattcgagc tttatgtacc cgcttgcgca ttcctaatga
     3721 ttatcgcgat ttggccgtta cagtggcgcg agctcatttg aatattcata gggtatgcga
     3781 attgcgtcct aacacagtcg tcaagctttt ggagcaggta gatgccttta gacgacctca
     3841 actatttcat aaaatattga ttgtttgcca agcagatgcc gagagttgtg gcaaaacagt
     3901 ggtctatcgc caaactcaat tatggaatga aatattatct gaatgtgtta aagtgaagcc
     3961 acaaactttt atcgtacaag gttatgaagg taaagcgatt aaggaggcca tgcatcaaag
     4021 tcgtgtggct tgtgtagagc gaattatgac ttcttggaaa tcaaatgaaa aataatcaaa
     4081 atttaatttg gattgatttg gaaatgactg gccttgagcc agaacaagat agaattatag
     4141 aaatggccac gattgtaacg gatccacaac ttaacattct cgcagaggga cctgtcatag
     4201 cggtgtctca gcctaaaatt cttctagaca gtatggatgc atggaataca aagcaacata
     4261 atcagtcggg attggtgaag cgagttttag agagcaatgt cagtgaatct caagctgaac
     4321 aattaacgat tgaattttta aaacagtatg tggataaggg aaaatcaccc atgtgtggca
     4381 atagtatctg ccaggataga cgttttttat ataaatacat gcctgagttg gctgcctatt
     4441 tccactatcg gaatttggat gtcagttcgt tgaaagagct ggtattgcgc tggcgtccag
     4501 aattaatgaa tggagtagtt aaggaatcga aacatcttgc tttggatgat ataaaagatt
     4561 caatcaatga gctgatttat tatcgacaac attttattaa tttgccggaa gtaaaaaatg
     4621 acaaatagag aacgtttaga attacctaat ggcgctaaaa agttattact tcattcctgt
     4681 tgtgctcctt gttcaggtga agtaatggaa gcgcttattt tttctgagat tgactttact
     4741 atattttttt ataaccccaa tattcatcct ctgcaggaat atgaaataag aaaagaagag
     4801 aatattaagt ttgcccaaaa gcatagtatt ccctttatcg atgccgatta tgataaagaa
     4861 aactggtttg ctcgagccaa aggattggaa tgggagccag aaagaggcaa gcgttgtact
     4921 atgtgttttg atatgcgatt tgaaagaaca gcactctatg cttatgagca tgactttcct
     4981 gttatcagta gttctttggg tatttcacgt tggaaagata tgaatcagat caatgattgt
     5041 ggagtgcggg cagcaagtca ttatcctgat ttgatatatt ggacttataa ttggcgaaag
     5101 aatggcggct ctgagagaat gtacgaaatt gccaaaagag aagcatttta caaacaagag
     5161 tattgtggtt gtgtttattc tttacgagat acgaatgcct ggcgcaagca aaatacaaga
     5221 gatcgcatta aaattggact taattattac tctgaaagca aaaacgaagg agaattatga
     5281 gtaaacattc gcatgatgga gcacaccatc atgagcatgg ggaagtgact tatgacaggg
     5341 cttttataat tgcaattact gcgaatggcc tttttgttgt gatgcaaatt atttttgcct
     5401 atttagcgaa ttctaccagc ttgctagcag atgcttttca taatttaggt gatgtattag
     5461 ggctgatttt agcatgggta gcaaccggat taatgaagag aaaacctacc cataaagcaa
     5521 cttatggatt taaaaaaacc tctattttag cggcattggc caatggagcg ttactggtat
     5581 ttacttgcgg gatcatagcg acagatgcta tgtataagtt attttcaccg actgaaatta
     5641 aagccgtctc tgtgatgata gtcgccgcga ttggtatagt cgtgaacttc tctacagcgc
     5701 ttttgttttt acggggctcc gatgatctca atattcgagg agcttattta catttatttt
     5761 atgatgcctt gatctcggta ggtgttgttt tatcagcggg tgtattatac ctgacgggtt
     5821 ggttatggat agatccggtt gtcggactat taattgcatt agtgattttg aagggcactt
     5881 ggtcactatt tgctgatagt ttcagattaa ttattgatgg tgttccccgt gatatatcct
     5941 ggattaaagt cagtgatttt ctcttggcta tacctggcgt aaaatctatt catgatttac
     6001 atatttgggc aatcagtacg aaagaaaatg cactttctgt tcatttattt atgccagagg
     6061 agtcattatc tgatgatttg cgtagagagc tggttgaaaa attaaagcat gatttcagta
     6121 ttcagcatgt cactatccaa gttgaaaaaa cagaagcgga atgcaatgac gcatgccata
     6181 atcccaggat aatcatttaa ttggggctgg atttacgacc cctaatttct ttgtttaaaa
     6241 aagggtttaa tttattcatt aattgtatct aaacgccccc attaaaaaca ttttcgtctt
     6301 gcatttcgag ttatctcttt cagtttttta atcaattagg ataattcatc ttttgttatc
     6361 aactccagac cttttttata acaagagcca ctggataagg ggtcatttag cttttcatgg
     6421 agtttaccaa gttctataaa tgctacgggc gtggggttta gctcaataga ctgttcaaga
     6481 taatgtttgg cttttcccca taaatgccga gagaggcaca ttctccctaa acataggtac
     6541 aatgcggctg agtgaggatt ttttttgagt aatgattcag caaacaacaa ttgattttct
     6601 tcacaaggga gcaggctata aagctcaatt aaccgattat ctaactcttt gcgaaggcat
     6661 cgccttaaat gatattctgc ttgatcgtaa tcctgattcc taatcagaaa gcgggcatat
     6721 tctgttgtaa tcgctgtatc gttaaccagt gtttttggca gcgtctgata caatttggta
     6781 atgccatctg attgattgtg ttttgccaaa tcaatcatgg cttgcaaata agtatttttt
     6841 tgtaattgtt caaaagcttg atctgaaata atgtggttct tttttaaatc aggtaaaaga
     6901 gcgatcaact gctgccaatc ttttatttct tcataaagat tcattaataa tttcaagaca
     6961 taaggatggc gtggtgctaa atcttgaaga tgctttaatg tggccaatgc ctgttcccat
     7021 tggtgattgg ccaattgtaa ttgcgcctgg gttaattcaa cagcaatttt cgcttcaggc
     7081 atagattgct gtgcttccct aagataatga tcgcgaagct tgctatcccc cattttttga
     7141 gcagctctcg ctgctgttaa ataattaagt aaaggagtgt cggtgtttgg cagagcttgt
     7201 attaaatgat tttttgcttt ttgccaatag ccctcgctat attctatcag tccctttcga
     7261 gtcgtggctt gagctttttg tgccaagcgc cgtgagtgcc atttattgac catgcctggc
     7321 attcttgtaa ttttattgca aagctgaacc aggccataga ttaaaaaaaa cagaaaaaac
     7381 aaaccaaaaa cagagaccca taaggtggtt tctatagtcc agttattaat agctattaag
     7441 acataacctg gatccttatt gagttgaata ccaaggaaca cagagagcag taaaataata
     7501 aaagcaaaca gaactcgtat cattcttagt tttctccttt cttgctgttt gcttgatcga
     7561 ccagcaattc tttattgtca atcatttgat tgattagcgg gatggcttgt cctacgacag
     7621 gtttttcctg agttaatttt acattttgca gagtggataa ttgcttaatc aaagcgtcag
     7681 tgttttggga agattcatta aatactcttt taagatttgc aatggcctgg tttaaagcaa
     7741 actggtagac tgcggggtta ttatttaaaa tagcccactg cgcttcctga aggttaaggc
     7801 ggatattctc ttttattgca gattcatata gaggggaaat aaggggttga atattttcat
     7861 tgtggcgacg aataacaacc aatttctcca gaaaattcac actgtcttgc aaccgatttc
     7921 gccatccagt tttgccagac tcttcagact tggtgatatg gtttagcact tcctgttgat
     7981 ctactacgga ttgaatagaa agattattga tagccgcttg agccgcatcc aattggctta
     8041 atatccctgt gatatcaaca acagaaattg actttaattg gacaatttct ttggcgattg
     8101 tctggcgaat agtaaatatt ttggggatat tcatgccttt cagtagggca tcagcttgct
     8161 gtaaaagggc gacagaggta ttaaaattat cactccaatg aacattgatt tgtgctaatt
     8221 caagataata acgtgctttt aaaagaagcc aatcattatt ttgatacaat ttttgcttca
     8281 tagctgtttg taattcttta tttaagctat ccattttgct tgttaaatca ctctgaattt
     8341 gctgaagatg attggcattg ttatccagga gcttttggac tgtattttgc tctgctttta
     8401 ttcttcccaa ttcatcagtt aactttttgt tttcaacaag ctgatggttt tgaaattgtt
     8461 tgtccaattg aatggaataa atagagatga tgatagcgag gagagccaaa agcgatgtca
     8521 agctgattat taaggatagg tatttactaa cgaagaaagg ggctttcatt tgttggttga
     8581 tgtcgggctc aactttagat gattgttgcg gctgaattgt ttttttctgt tcttcattgc
     8641 tatcggccat ggattaatcc ttatagtcaa ataacgtacc tatcatcctt tctggatggc
     8701 taataagaat ctttttaatt ccaaataaag aagctacctt tgcaagtcgg tcactaatga
     8761 ccaggcaagg tttgttttgt aaccacattt gcccctctat gctaaacatt ttaaaaagat
     8821 tatgtatgga ctgctcactt gttaacagta taatgtccgc caggtcatcg cgccacagag
     8881 aattaagaaa ttcttgatcg atggttggca tgactcgttt atagactgac agaagacata
     8941 gcttcgctcc cttcagcatt aaatattctt caatcagttg cctgcctcct tcccctttca
     9001 caagcagaat cgtctgattt cttatttgtt gcaacgaatt gagagtcagt aaatgctcgc
     9061 tgtcaggtaa ttcggggaca tcactaacct ggatattgaa ttcattgagt actctggagg
     9121 tgcctttacc tatagcagct acccggatgc tatttggcca attgatgttt ttggaagcca
     9181 gttccatcat gaaataacga accgcatttc cactgacaaa aatggcctga tttactgagg
     9241 tcaaatcggg caatttattt atccaattgg tctcagacgc cactatttcc atcgtagggc
     9301 agtcgatggg tatcccgcca gactcgataa tttttttatt tagtacttct gcttgctccc
     9361 tgggtctggt gtttaatatg cgaagaccat taagtgattt ggtcatgatg gtatcgtgct
     9421 cagtaaagtc attgcacctt ttgtcattaa tgactcggta cacctgtctg caataatttc
     9481 agcttctgca attttccctt tttgctcatc ctcaatcatt tgagaaccat ccatattaag
     9541 tacttttgct ctcaaaagga ggaggttctc ctcagtaatg gtacaataaa cagcaaaggg
     9601 tatatggcaa ttgcctccta attttgcgtt gacgcgtcgc tcggtatgaa cacaaagggc
     9661 ggaaattgga tcatttaagc catgaatcag ctcttggatt tccacatcgt cagttcggca
     9721 ctcaatacat aaagcgccct gagcacaagt aggcagcata atgtcatcgg gtatgagttg
     9781 ggtgatgtta tgtgccaagc ccattctttc caagcctgcg gcagcaagaa taatggcttg
     9841 atactctcct gactccagtt tgctgagtct tgtatggatg tttcctctta atgttttgat
     9901 ttgcaaattg ggattgtaag ccaatagttg agattgacgt ctaagacttg atgtaccaat
     9961 aattgcgttt ttggggaggg catccagact cttaaattgg ggacttatta aagcgtcaaa
    10021 agggttatct cttttgcaaa tggcggccaa cgagaggcca tccggaagtt gtgctggcat
    10081 gtctttagtc gaatgaacag ccaagtccgc ccttttatcc aataatgctt cttctaactc
    10141 tttaacaaat aaccctttcc ctcctgcgga taataattta tctttaagaa atcgatcgcc
    10201 agaggttatc atgggtaata attcaatgct tagatttggc cattgcttta caagcatttc
    10261 ccgtacatga ttagcctgcc atagtgcaag cgggctctgc ctcgttgcga ttcgtatgat
    10321 ttttgcgctc atggaataga cggtatatgt tctttgggta aagataataa cattgattct
    10381 aatttaaaaa gaaccccatt gagataatag gtacgcaaat cttgtcgggc aattgattta
    10441 taaatgagca ctcctaccgg gttgagaatg ggtttatagt gccaacc
//
LOCUS       DASLOE010000108        10297 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.109, whole genome shotgun sequence.
ACCESSION   DASLOE010000108 DASLOE010000000
VERSION     DASLOE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.109"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(303..1316)
                     /locus_tag="U6309_002070"
     CDS             complement(303..1316)
                     /locus_tag="U6309_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN5505508.1"
                     /translation="MKAIGYYHSLPITDNKALMDLDVSVPKATGHDILVEIKAVAVNP
                     VDTKVRRNVQPEPHQPKILGWDASGVVTEIGSEVTMFKPGDEVWYAGDLTRPGSNCEF
                     HLVDERIAAKKPKSLSFQEAAALPLTSLTAWELLFDRLQLSAEDKGILLITGAAGGVG
                     SILVQLARQLTQLTIIGTGARPESIQWILDNGAHHVIDHTKDMKAQLQNLHYSEVDYV
                     ISLTHTDQHAKSLVEVLKPQGKFALIDDPQDLDIKLFKLKSISIHWEMMYTRSMFKTA
                     DMIKQHDILTEVARLVDAGKIKTTIAECLGLINADNLRRAHALLESGTSRGKIVLNGF
                     KSI"
     gene            1636..2163
                     /locus_tag="U6309_002071"
     CDS             1636..2163
                     /locus_tag="U6309_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEN5505509.1"
                     /translation="MAIFKNLVLTLVSSALLVTPCFSAGLTLESPAFASNAAIPQKFT
                     CSNLNYSPPLIWHDPNLGTQSYVLIVHDPDAPTGNWIHWVLFNIPAQVKQLTEGATTP
                     AGATSGLNSWNTTGYSGPCPPAGTHRYYFTLYALDTYLNLGPEATAQDVVNAMKGDII
                     DTAELVGHYSKNASK"
     gene            complement(2325..3374)
                     /locus_tag="U6309_002072"
     CDS             complement(2325..3374)
                     /locus_tag="U6309_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HEN5505510.1"
                     /translation="MTNDHYVSKKKMFAVVFCVFAPNEVLLANDTAVGGEGSLPIPVS
                     QPHIKMVSELIRISGKNLNEPQMNGSWHYNCNFTFKNTLNKEVTISMAFPFPINDGNS
                     EIALPAGQQTNVGQALVYDFLVIVNDKQVPAQRGNIAPDQNKGLYYEDAYFWKTTFPP
                     LATVNIHHDYSTGATYDVMGYHWVRYVLKTGALWQDSSIGHTRLEVIPNTPTRLCSEI
                     DPKADYLNPTPSGMSISGSRADRKYIWDLRHFQPQADLSLCLFTGISYVRYKVIYPWL
                     NSDDALGKLARLSNKELRFLRNTIYAQYGRQFQSPDLQEYFSKKWWYVPNPDYSDRML
                     NEEDKKLLSMINQAK"
     gene            complement(4046..4645)
                     /locus_tag="U6309_002073"
     CDS             complement(4046..4645)
                     /locus_tag="U6309_002073"
                     /inference="COORDINATES: protein motif:HMM:NF025793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEN5505511.1"
                     /translation="MEKNKIIDKLLAITEQTIVPMTQKSILQGNKIFGAAILNKADLS
                     LIIASTNHETENPLWHGEIHTLKKFYEIPKNQRPLAKDCLFFSTHEPCSLCLSAITWG
                     GYDNFYYLFDYYDTRDLFHIPHDLKILKEVFNCDNGRYSTTNSYWTSYYLLDLINSSD
                     VKSKSDFLSRVAALKDIYNELSEKYQIASKLNESEIIQK"
     gene            complement(4675..5679)
                     /locus_tag="U6309_002074"
     CDS             complement(4675..5679)
                     /locus_tag="U6309_002074"
                     /inference="COORDINATES: protein motif:HMM:NF012575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEN5505512.1"
                     /translation="MFITQQPLFKKFWYPVIPIHNIKEGPASFVLLGTKLVLWLNELG
                     EPCSAVDRCAHRSAQLSKGWVCEGHITCPYHGWSYNKEGKCVDFNQGCGIIPASYKIE
                     TFKAKEKYGYVWVCLDEPIIDIPAYPEALMENYRLVECFYETWNTTSLRVVENELDMA
                     HFAVVHRNTFGNPAKPLPLSLEIKELDDYRLNVHSELSVCVPEQQQKNTHEYLSDNQR
                     TSTRLMDVTWTMPFNIKLSIRYPNGLHHVIINNPTPIDDNHIQVVQFCFRSDSEAEVS
                     REDLIKFERKILDEDRLVLETTDYNYPLFHEYAEVHMPTDKAGILVRKKIKKMIDRYT
                     "
     gene            complement(6237..6641)
                     /locus_tag="U6309_002075"
     CDS             complement(6237..6641)
                     /locus_tag="U6309_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEN5505513.1"
                     /translation="MVQHDIIQLPPKRYGAHLITNEVMQKITLRGTGLLHLFIQHTSA
                     ALALNENASPDVLIDLNHFFTQLVPEHQHYRHDIEGPDDMPAHIKSILCGSSLTIPFQ
                     ENKLLLGVWQGIYLMEFRKQAGARTIVASQLI"
     gene            6841..8115
                     /locus_tag="U6309_002076"
     CDS             6841..8115
                     /locus_tag="U6309_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylpolyamine aminohydrolase"
                     /protein_id="HEN5505514.1"
                     /translation="MAKGKGFFKKAKTGKNKSSATPCAEECVIQIPSEIDNEQMHRMP
                     AGGEEDQYLRIKHMSALIKKYGNLPIITTRETRLPDYWLDLFAAIDEGDTPKAHALFH
                     LLPQEDIILRALRAVHSEDYLYQLIKYCIQAKHFGFKQLNADLVVTPKTFEILIKDCA
                     TTLFNPAKTHFSFGLPSHHAYTQMGSGFCLINKTAMLIKQAELSSVQPPRFVIIGTDV
                     NRDNGLCDILRHSFSHLSICHIDVFDSRVYPQQDFSYINNEFNSEGVDVGKNIHVWHH
                     NDLSYYAVDLSLTSRKSVGVHPALLFALEQFKASIREAKVKGQKIALYLPTGWDSHED
                     ETAYCGKFVKGRMLGKTAAHQFRFNDGDLSYFYESIFTLYNENKDCIDTIYWGLEGGY
                     DRTMYERELTLLLQVIEKQLLPQDSNSHNMSC"
     gene            8386..8802
                     /locus_tag="U6309_002077"
     CDS             8386..8802
                     /locus_tag="U6309_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4949 domain-containing protein"
                     /protein_id="HEN5505515.1"
                     /translation="MMFQSKLATFFSALILAGSAFASQQGACPDINVIKAEGLPMAEE
                     IGMNYYLAYNISDYSTSSVWGFFIAPIEADSEEGALEASNEVLSMMNAPGVPHQQDED
                     IICSYDTGTPNLIAAAIKDGSQISPMKLKQYFKSAR"
     gene            complement(8898..9821)
                     /locus_tag="U6309_002078"
     CDS             complement(8898..9821)
                     /locus_tag="U6309_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEN5505516.1"
                     /translation="MNILLAGGSGLIGQELVRGMGEEHHFTVLGRDKDAIQKIFTTKA
                     SSLSWDELNTQDASRFDAVINLCGVNIAISRWTPKIKEEIISSRVQTTENLLYWILQQ
                     NAKPRFYCANAVGIYGLQENGDATDLTEDTAINWEHPKDYLSEVGIRWQQALQKAEEA
                     DLAVTSTRFGVVLKKGKGFLGKLYPSFICGLGSTIGDGRQYLSWIDSEDLVNAYRFLL
                     NHPEITGPVNVTSPNPCMQREFAKAFAKSLHRPLLLWTPAFVIHLLFGEMGDCLINHG
                     QKVKPKRLLEAGFEFKWPGIEKALKHQYSAA"
     gene            complement(9899..>10297)
                     /locus_tag="U6309_002079"
     CDS             complement(9899..>10297)
                     /locus_tag="U6309_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEN5505517.1"
                     /translation="MEYLLESIKQNQSYMVAYFLKDIPPEHLKTIYMSVSAVERTNLH
                     ILRQLQNYGVPFSDMSKAVFEQKEHHFVGLRRILGVFLCKFTDFLKELLFNDCGIHCH
                     RAEESGVKKSFFAFPKKLVGVEQLQIPKLY"
BASE COUNT         3247 a         2059 c         1816 g         3175 t
ORIGIN      
        1 ccctgtatta attaatatag aacacgacaa aacatcatgt ccaattttta attaaaagat
       61 atttttttaa cacaaataga tttgcgtaaa actccaaggt caaggcaaaa aattttttta
      121 atgaggggct tagataaact aaatgggtga tcctccggat actgctgctt gtcagcgata
      181 ccaaccaagc actctctaga tgcctcagac aagctgaggc acgtaggcta tttagagaat
      241 catttaaaaa tcaagtgact ctatctgttt cattccaggc atcgtcgctg tttcgagaat
      301 aactaaattg acttgaatcc attcaaaacg atcttgcctc tggaagtacc actttccagt
      361 aaagcatgtg ctcttctcaa attgtcagca ttgatcaatc ccaaacactc ggcaatagtt
      421 gtcttaattt taccggcatc caccaatctg gcaacctccg ttaaaatatc atgttgctta
      481 atcatatcag ctgttttaaa catagagcgg gtgtacatca tttcccagtg aatagaaata
      541 ctctttagtt taaataactt aatatccaaa tcctgagggt catctatcaa tgcaaatttc
      601 ccttgaggtt tcagtacctc aactaacgat ttggcatgtt gatcggtatg ggtcaaactg
      661 atcacataat ccacttcaga ataatgtaaa ttctgcaatt gggccttcat gtctttagtg
      721 tgatcaatga catggtgggc cccattatcc aggatccact gaattgattc agggcgggct
      781 cctgttccga tgatagttaa ctgggtaagt tgccttgcca gttgtaccaa aatagaacca
      841 acccccccag ccgctccagt aattaataga atacctttat cttctgctga taattgcaat
      901 ctatcaaata aaagctccca agctgttaaa ctggttaaag gtaatgcagc agcctcttga
      961 aaacttaacg actttggttt tttggctgcg atgcgttcgt caacaagatg aaactcacaa
     1021 ttagaccccg gtctggttaa atcaccggca taccacactt catcacctgg tttaaacata
     1081 gtcacttccg atccaatttc agtaactact ccggaagcat cccagcctaa tattttgggc
     1141 tgatgaggct ccggttgaac attacgacgc accttggtat ctacggggtt cactgcaaca
     1201 gctttgattt caactaaaat atcgtggcca gttgctttgg gaacagaaac atccaggtcc
     1261 atcaatgctt tattatctgt aatgggtaaa gagtggtagt aacctattgc tttcattgaa
     1321 attattcctt taaaaatact ggagtttcat tataggttta gaaaatttta taaacaacag
     1381 cacaaggcat ttctcatatg aatgggactt acaccccccc ccaatgtcga ggaagaaatg
     1441 tttttaatga gggagtttag ataaattaaa tgtccgaatt aaacattttt taaacaaaga
     1501 gattggtgct tttggtaaat cctgatgaaa aacaaatcac aagtgtatca atcgtaatct
     1561 ataatttaag ggaatctata aaagattaga atctcaataa tttcaggtca gtaaacaata
     1621 gaaacaggga aagtgatggc catatttaaa aatcttgtct tgacattagt cagctctgca
     1681 ttacttgtta caccgtgttt ttctgcagga ttaacattgg aaagtccggc atttgcatcg
     1741 aatgcggcaa ttcctcaaaa attcacatgc agcaatttaa attattctcc cccactaata
     1801 tggcatgatc ccaatctggg aacccaatct tatgtgttaa tagtgcatga tcctgatgct
     1861 cctacaggta attggataca ttgggtattg tttaatatcc ctgctcaagt gaaacagctc
     1921 acagaagggg caacaactcc tgctggcgca accagtgggt taaacagctg gaataccacc
     1981 ggttatagtg gtccctgccc gcctgcaggt acacaccgtt actattttac actctatgct
     2041 ttagacactt accttaatct tggcccagaa gccactgctc aggatgtggt gaatgctatg
     2101 aaaggagata tcatagatac ggctgaatta gtaggacatt atagtaagaa tgcatctaaa
     2161 taaaattgca acaatgcttt tctcaagcgc tcctgcaaca cacacaagaa ttggcataaa
     2221 acaaaaatag acccgggtgg taatagtcac ctgtcataat cagcttttat taatttagag
     2281 gcctcatatg ggatttatta taaagagctt atcagcgatt gagtttattt tgcctgatta
     2341 atcattgaca ataatttctt atcttcttca ttgagcatac ggtctgaata atcaggattt
     2401 ggtacatacc accatttctt gctaaaatat tcttgtaaat cgggtgattg aaattgacgc
     2461 ccatattgcg cataaatagt atttcgtaag aatcgtaatt ctttattact caatctggct
     2521 aatttgccta atgcatcatc tgaatttaac caaggataaa tcaccttata gcgaacataa
     2581 ctaatccccg taaataagca aagtgacaaa tctgcttggg gttgaaaatg ccgtaaatcc
     2641 cagatgtatt ttctatccgc tcgtgagcct gaaatgctca tccctgatgg ggtaggattt
     2701 aggtagtctg cctttggatc tatctcagag cacaaacgcg ttggtgtatt gggaatgact
     2761 tcaagtcgag tatgtccgat actgctatcc tgccaaagag cacctgtttt taaaacataa
     2821 cgaacccaat gataccccat cacatcataa gtagctcccg tcgaataatc atgatgaata
     2881 ttgactgttg ccaagggtgg aaaggtggtt ttccagaaat aggcatcctc atagtaaagg
     2941 cctttattct gatccggggc gatattacca cgctgtgctg gaacttgctt atcattgact
     3001 ataactagaa aatcataaac caatgcttga ccaacattag tctgctgtcc cgctggtagg
     3061 gcaatttctg aattcccatc gtttataggg aagggaaaag ccattgaaat agtaacttct
     3121 ttatttaatg tatttttaaa agtgaaatta caattataat gccaggaacc attcatctga
     3181 ggctcattta aattctttcc actgatcctg attaactcac tcaccatttt tatatgcggc
     3241 tgagaaacag gaatgggtaa agatccctcc ccacctacag cagtatcatt agctaataaa
     3301 acctcattgg gcgcaaaaac acaaaaaacc acagcaaaca tctttttttt agatacgtaa
     3361 tgatcattcg tcatgaggct gtcctggttt caaaataata taaagtatag ttaagaacag
     3421 ggggtttttc tttcttatga agcaacaggt ttattactcc ttgttgacga gattatatct
     3481 cttaaacaag gtgtacggct attcgagaaa agatcaaacc taatgatttt cgtattattg
     3541 aacaatataa aatccctgat agagtttttg ttgctaatag ccaaaaaata tctcataaaa
     3601 ctcaacaaga attcgtaaat gcaatgctaa aaataccaaa caaaactata caggtctttc
     3661 ttttagtgac gtaacaccct atcaacgaaa cggctataag gctgttttgt cacttctcaa
     3721 acaatatcgt caattgcaca ttaaacctaa aacttgtgaa aaaatgacca tcaataagaa
     3781 atcaaggatt tggtcacact caaaacatcc tagtactcta tgaattattg attttcgata
     3841 gcagggcttc aaccctgcac tatacacatc gggtttttgg ccaactaact ggactatgct
     3901 ggactgtttt gttgtagcgt ccccagataa gggatctttt gagttttccg caatagcagc
     3961 gactactgtt aaaataccag cataaagcaa ttcaaaaact aattaagagg gctataggaa
     4021 atacgcacaa aaatcggtac atcttctatt tttgaatgat ttcactttca ttgagcttag
     4081 aagctatttg atacttttca gacagttcat tatagatgtc ttttaaagct gctactctgg
     4141 agagaaaatc agattttgac ttcacatcgc tagaattgat taaatcgagc aaataataac
     4201 ttgtccagta actattagtg gtagagtaac gtccattatc acaattaaat acttctttta
     4261 atatctttaa atcatgaggg atatgaaata aatctcgagt atcgtagtaa tcaaaaagat
     4321 aataaaaatt atcgtatccg ccccaggtaa ttgcggaaag gcaaagagag catggctcat
     4381 gagtcgaaaa aaaaagacaa tcttttgcta aaggccgctg attcttggga atttcataaa
     4441 atttttttag tgtatgaatc tcaccatgcc acaagggatt ttccgtttca tgattggtag
     4501 aagcaatgat taaagacaaa tcggctttgt tcaatattgc cgcaccaaat attttattac
     4561 cctgcaagat actcttttgg gtcattggta ctatagtttg ttccgttata gctaataatt
     4621 tatcaatgat tttatttttt tccataatag tccattaatg aatgaagatt aagtctaagt
     4681 atacctatca atcatttttt taattttttt tctaacaagt attcctgcct tatcagtagg
     4741 catatgaact tcagcatatt cgtgaaacag gggataatta tagtctgtcg tttccagtac
     4801 caagcgatct tcatctagaa ttttacgttc aaatttaatt aaatcttctc gtgatacctc
     4861 ggcctcgcta tcactacgaa agcaaaattg caccacttga atatgattgt catcaatagg
     4921 ggtagggtta ttaataatca catgatgcag accattagga taacgaatag agagcttaat
     4981 attaaaaggc atcgtccaag tcacatccat taaacgagta gatgttctct ggttatcact
     5041 taagtattca tgagtatttt tttgttgctg ctcgggaaca catacactta attcagaatg
     5101 aacatttaaa cgataatcat caagttcttt aatctctaga gatagaggta atggtttagc
     5161 tggattaccg aaggtattac gatgtactac tgcaaaatga gccatatcca attcattttc
     5221 aacaacccgt agacttgtgg tattccatgt ctcgtagaaa cactcaacaa ggcgatagtt
     5281 ctccatgagt gcttcaggat aggcaggaat atctatgatt ggctcatcaa gacaaaccca
     5341 aacataaccg tatttttctt tggccttaaa tgtctctatt ttataggatg caggaattat
     5401 cccacatcct tgattaaaat caacgcattt accctcttta ttgtaactcc agccatgata
     5461 aggacaagta atgtgtccct cacagaccca tccttttgat aactgagcgg aacgatgggc
     5521 acaacgatca actgctgaac aaggctcacc cagttcattg agccacaaaa ccaatttcgt
     5581 tcccaataat acaaaagaag cgggaccctc ttttatatta tgaataggga taacgggata
     5641 ccagaatttc ttaaataaag gttgttgagt tataaacata taacctcccg ctttgttttt
     5701 agaccacaac ctaataattt gtgatcaaaa aaaatactta ttgtcttatt tatagtttac
     5761 aaacagaatt tggacaagat tttatctctc atgccattct ttgatagagg gactaggtac
     5821 gctaatttat aaaattttta gaaaattata attatttcct ttataaatca gattttttgc
     5881 taagatgggg agacgacccc ccccccatta ttcatgctct cttgtggata atttttttgt
     5941 aaatattctc tccattatac tcaataattt tgataaaacg ttattagtcc ttaccacaaa
     6001 ggcagggaaa aatcatcaga ccacatagcc tgctttaatt tacctctcat actatctttt
     6061 ttaggatgta atcgcgctgg atttaaggca aattgcctgg ctgttgcaat attttcagcc
     6121 gcattttcgc gcctgactct tgaatcatcc tctttatatg ttacgtctaa aactagtgtg
     6181 ctttattttc tacttcccat taagctcaat cgtgagtaga gttatgccct atgatcctaa
     6241 atcaattgac tggcaacaat tgttcttgct cctgcttgtt ttctgaattc cattaaataa
     6301 atgccttgcc aaacacccaa tagcaacttg ttttcctgaa atggaatagt taaagatgag
     6361 ccacacaaaa ttgatttaat atgggcgggc atatcatctg gtccctctat atcatgacga
     6421 taatgctgat gttcaggaac aagttgggta aagaaatggt ttagatcaat caatacatca
     6481 ggggaagcat tttcatttaa ggcaagtgcc gcagaggtat gctgaataaa taaatgtaat
     6541 aatccagtgc ctctcaacgt aattttttgc atcacttcat tagtgattag atgagcacca
     6601 taacgctttg gaggcagttg gattatgtca tgttgtacca tagaccctcg acttaattgg
     6661 ataacggagg aattgtcaag tcagaacaaa atgaaattct gtccataaac tctatttgca
     6721 ttataaataa agtagcgcct cttgtcttga tagtcatgag cttaacaaat ctctggatta
     6781 atatctctca ctcgcctatt ctaaaatata ataaattaaa caagctattt aggaatggat
     6841 atggccaagg gtaaaggatt ttttaaaaaa gcaaaaacgg ggaaaaacaa aagttctgcc
     6901 actccatgcg ctgaagaatg tgtcattcaa atcccatctg aaatagacaa tgagcaaatg
     6961 caccgcatgc cagcaggcgg tgaagaagat caatacttac gtataaagca catgtcagca
     7021 ctgataaaaa aatatggaaa tttaccaatt attacaactc gggaaaccag gctaccagac
     7081 tattggctag acttattcgc agcgatagat gaaggagata cacccaaagc ccatgcgctt
     7141 tttcatctgc taccgcagga ggatattatt ttacgagcat tacgagctgt tcattcagaa
     7201 gattatcttt atcaactcat taaatattgt attcaagcga aacattttgg ctttaaacaa
     7261 ctaaatgccg atctggtcgt taccccaaaa acctttgaaa ttttaatcaa ggattgtgcc
     7321 acaactctgt tcaatccggc aaaaacccat ttttcttttg gtttaccaag ccatcatgcc
     7381 tatacccaaa tgggaagcgg tttttgcctt atcaataaaa cagccatgct gataaaacag
     7441 gctgagttga gttctgttca accacctaga tttgtcatta taggaaccga cgtcaatcgc
     7501 gacaatggcc tatgtgacat tttaagacac tcattttccc atctatcgat ttgtcacatc
     7561 gatgtgtttg attcaagagt atatccccaa caagattttt cctacattaa taacgaattc
     7621 aattctgagg gagtagatgt agggaaaaac atccatgtat ggcaccacaa tgatttaagt
     7681 tattatgcgg tggatctcag cttaacgtcc agaaaatcag taggcgtgca ccctgcgctt
     7741 ctttttgctt tagagcaatt caaagcatcg attagagagg cgaaagtaaa agggcaaaaa
     7801 attgctttat acctcccgac cggatgggat tctcatgaag atgaaacagc gtattgcggt
     7861 aaatttgtta aaggccggat gctgggaaaa actgccgccc accaatttcg ctttaatgac
     7921 ggtgatctaa gctacttcta tgaatccata ttcactctgt ataacgagaa caaagactgt
     7981 attgacacca tctattgggg cttggaaggt ggatatgaca ggaccatgta tgaaagagaa
     8041 cttactctgc tgttgcaggt cattgagaaa caattgctac ctcaagactc caactcgcat
     8101 aatatgtcct gttaatggtt attatttcaa attttagtaa tgatagcgcg aactgttttg
     8161 gccatgaaat agttactatt gatttcaaaa acgattagcg atttaccata ctgtgttgat
     8221 tgctggttct ggacgatagc attagtaatc aatgctcata cctccgagaa taaggccagt
     8281 ttgttttccg tttaccctcc aatctcaaat tacaacatta ttgtaaatct tctaggcaaa
     8341 tgatactctt tgcgcggaat taaaaataac tcaggagtaa aaaacatgat gtttcaatct
     8401 aaattagcga ctttttttag cgctttaatt ctcgccggtt ctgcttttgc ttctcaacaa
     8461 ggtgcttgcc cagatatcaa tgtaatcaag gcggaaggtt tgcctatggc tgaagaaatt
     8521 ggcatgaatt actatctggc ttataatatc agtgattaca gcacctcttc tgtgtggggc
     8581 tttttcattg ctccaattga agcagattcc gaggaaggcg ctttggaagc atcgaatgaa
     8641 gtgttaagca tgatgaatgc tcctggagtt cctcatcaac aagacgaaga catcatttgc
     8701 agttatgaca caggcactcc taatttaatc gccgctgcta tcaaagatgg ctctcaaatt
     8761 tctccaatga aattaaaaca atactttaag agtgctcgtt aattagtgct cctttaaata
     8821 ccctgtcaag tattcagtaa cttacagggt attttaaata tccctccaaa tcatgaatcc
     8881 tcttaaaaat gctgttacta tgcagcggaa tactggtgct ttaatgcctt ttcaatgcca
     8941 ggccatttaa actcaaaacc agcttccaaa agtcgtttgg gttttacctt ttgcccgtga
     9001 ttgattaaac aatcacccat ttcaccgaat aaaagatgaa ttacaaaagc aggagtccac
     9061 aataataatg gcctatgcag agacttggca aaagctttag caaattctct ttgcatgcat
     9121 ggatttggtg aggtcacatt cacaggtccg gtaatttcag gatgatttaa caaaaaccga
     9181 taggcattga ccaaatcctc actgtctatc caggaaagat attggcgacc atccccaata
     9241 gtacttccta atccgcaaat aaaactggga tagagctttc ctaaaaagcc ctttcccttt
     9301 tttagaacaa caccaaagcg tgtggaagtc acagcaaggt ccgcttcctc tgccttttgt
     9361 aaggcttgct gccaacgaat tcccacttca cttaggtaat cctttgggtg ttcccaatta
     9421 attgctgtgt cttcagtcaa atcagttgcg tcaccatttt cctgtaaacc ataaatccca
     9481 actgcattgg cgcaataaaa gcgaggtttg gcattctgct gcaaaatcca ataaagcaaa
     9541 ttttctgttg tttgtactct cgaactgatt atttcttctt ttatttttgg tgtccatcgt
     9601 gaaatggcaa tattgacgcc acataaatta attacagcgt caaaccgact ggcatcctgg
     9661 gtatttaatt catcccagga caaagaggat gcttttgttg taaaaatctt ttgtattgcg
     9721 tccttatcac gccccagcac agtaaaatgg tgctcctctc ccattccacg aactaactct
     9781 tgccctatta atccagagcc tcctgccaat aagatattca caatgaatca agctcctttc
     9841 aggcatctta tttaattaat ataaactgat gagtactgtt tgtcattagt acagtgcctc
     9901 aataaagttt ggggatttgt agttgttcaa ctccaaccag ttttttaggg aaggcaaaaa
     9961 atgatttttt tactccactc tcttctgctc gatggcaatg tattccgcaa tcattgaata
    10021 agagttcttt aagaaaatca gtgaatttac acaaaaaaac acctagtatc cgtcttaaac
    10081 ctacaaaatg gtgctctttt tgttcaaaaa cagcctttga catgtctgaa aatggaactc
    10141 cataattctg taactgacgt aaaatatgca gatttgttct ctcaacagca gagacgctca
    10201 tatagattgt ttttaaatgt tccggtggga tatctttaag aaaataagcc accatgtaag
    10261 attgattttg ctttattgac tcaagcaaat actccat
//
LOCUS       DASLOE010000109        10256 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.110, whole genome shotgun sequence.
ACCESSION   DASLOE010000109 DASLOE010000000
VERSION     DASLOE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10256
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.110"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(230..2512)
                     /locus_tag="U6309_002080"
     CDS             complement(230..2512)
                     /locus_tag="U6309_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505518.1"
                     /translation="MFPYVSLAHFNELFEIMKPNLFYFVLTQILAESPNFKLLDEVRP
                     VHDITLKDKKYYRLELPLANIAAEGFRLHDAHISLYEKTDPHNPNLGLSHFTAIFHDQ
                     NGQTYRMHLFLNRFDALACPATWELLDDDEQYVKVSPPENLEYFMHSVWQLGLPCLQL
                     LRKKQKEIEAKLLADYHRLNEETTLLSKDLDENRAVYLEKLDELIQTTKSLSQISESN
                     HWSREAIYLVKTHKYVSSMPEPILEPVNKGDEEQKKDDQAEVVALSPKQGATKPKISR
                     GNIGLAQSTLFSKSAKAKRPVDVKMERDISKIKKLFAQLLKTEDKKIKAVLLIDLHWK
                     IKDINLETEELLTKEQTQALNEIESRANKEAKSLLDRALFAGEFEYAQTLSPYYPLIN
                     NDVMVLALTQRKADLLSFLVTKVGLPINSYPIKTKVKTYSNAVEYCFSEHSESLVDCF
                     SVLIKNGASLMQPVGLHKLPLAHLLLSEIPRHPLYAALEQNKSLTLNNKQFYAHLINA
                     LKSCLLSDDIEGDQKIALEESIARYEHLKANVKNSSSLLSPQNQALADEISDITKKLL
                     SEAVAEAIESDEEISREKAMADKEYKELVRKVKAFYHKTGKNFAFQTLINSANQELKK
                     ELLEIDFNIDISFSELKKSVLENLSNERLMLSYCSELIDVQTTIMQISQGASRKNKNL
                     KKLNARHAELIELLTDLSQNTPQSMIKEANEFQDSFSQFQDLLNSLNQLEGSFSMLAG
                     VLNQFIGGSTSESESFGVKL"
     gene            complement(2903..3541)
                     /locus_tag="U6309_002081"
     CDS             complement(2903..3541)
                     /locus_tag="U6309_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505519.1"
                     /translation="MKYIFLFIAVIVASFNPAFASVLPSSKDAQLCTNAYNKLWHIFD
                     SVKADHRGDPAAQAAIFNVEFPKVLAEDFSLTINNLPVEPNSSETITVNVQGLPQVIS
                     VASEQITSWGQHHIAGPFSFKLLSESLPYKTRVYLMTTRDIDYTNSPENNGCAAFLAQ
                     KNMVCTVKNTGLTTRKAMLNSMNSHVVTTYVMPAPNCSLWIAPPVEDFPTEA"
     gene            complement(3785..5074)
                     /locus_tag="U6309_002082"
     CDS             complement(3785..5074)
                     /locus_tag="U6309_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEN5505520.1"
                     /translation="MSFYIQLASLSIIILYVLLLFRNVDPLVVTAVCVGLGFLWNIST
                     PIDIGNAMADALGSFMALVGFIIMLGRGLGEVLTHTQVSHTLVHQIVYGVGVNTQRRA
                     KIGIVLSSFIIVGLLGTLAGGLAILAPSLRPIAGSVGLSRPSLAVLMQASAEEALILG
                     PFAPPVVTLLGVTGLSYGTVFLYASLPIAVVTLITTWFMASRLQKLYINEPCEDENST
                     EPFTPNRQQKRATLIFLISFLFCVVYGLFTQAKTSYVIFVMLFLALITGLSCRLGLKQ
                     IFNLLFEGMQKSLHLFFLFILFDPFMALIHQAGGFNALTELLTPLINLGGKPVLSILI
                     GFTGAFGMPGAAEATIKMLHQLFSPSVIQMQLPLITFALCMLFATRVTNYAYPGANMF
                     AAMGFAGSENVKAMIQNGLVVTGVQVAFLIVYSLFIP"
     gene            complement(5074..6177)
                     /locus_tag="U6309_002083"
     CDS             complement(5074..6177)
                     /locus_tag="U6309_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEN5505521.1"
                     /translation="MNIKEIHIAELTIPLSRPFITAVRRTECVDDVVILIKTDCGKIG
                     YGSAASTPAITGDSKESIIAAIKNALGPPLIGRDISAFNLLLEMNDRAIGGNSSAKAA
                     IDIALHDLFAQYCGLPLYKQLGGNTNCINSCITISVKEVEEMVGDAIDLIQRGHKTLK
                     IKLGLNPVDDINRIRAIRQAVGNDITLLADANQGWSYEEALIAITAFKKENLNVPLVE
                     QPVSARNLPDLKTISDQVDCAIIADEACFSPEDALNIAKINACDGVNIKLMKSGGIEK
                     AQSIYHIAQAAKMQIMVGCMLESPIGVAAIASFALSKPDISYADLDPIFLIRENYILG
                     GAQSLSNKIILAEKPGLGIEGITQGLNLIGVIR"
     gene            6648..7460
                     /locus_tag="U6309_002084"
     CDS             6648..7460
                     /locus_tag="U6309_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEN5505522.1"
                     /translation="MYELNDTPYFIYSNLIQEYCSPLFNLTPIKGFTFCRTYGDKTAF
                     LLTSEYQFHIECIQHEKKISKLNMLRTPEYTQAGYFLNSPALDGKYSPFYESTAQKYG
                     YGFSFTIVRKNENHCDYFHFIGNRHQKDMNNYYLHNKWLLDKFCDYFLERAAKVLAYS
                     EKENRFTLTNTAEHPFPLKIHSSALDQFLKDYHFEQFGLKPVCLPKRQIECAQLLVRG
                     KTAEEIAIILNLSKRTVEHYINILKRKLNAHNKGLLITKLLHQYPDMFESIL"
     gene            complement(7490..7894)
                     /locus_tag="U6309_002085"
     CDS             complement(7490..7894)
                     /locus_tag="U6309_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505523.1"
                     /translation="MTSFFLRKNITKSAKVLLEKEAEKIFSRNGQGLTFLEKGYKVMT
                     YTKHPVQNDDLILAYSPNVIYSNERMNKIREVMRRRQCGFALFRVKEDTFCQVKQLKN
                     YPEDLEARDTSQFFVTSETLEPVKFLESSPPP"
     gene            8080..8847
                     /locus_tag="U6309_002086"
     CDS             8080..8847
                     /locus_tag="U6309_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505524.1"
                     /translation="MFFKTCEFQRNKSSDTVYPRIDIRQAIYKESISLTPEMAISMQK
                     KGGYQTDNDWKKMLMGDVTQNLLIQSVIGKKSLICAPPNINSMSLEDFHLWTKQPLPA
                     GTSQYLALPNAFADNLIHGYHFVEARNYHHLVQVGEVKCQKTGNTLYPLRKALAVFAI
                     SEWMQELPVLVARASANTEHGEGGAIQLIVPLELLRLLHSNVKLLITQEDNEYSVEKK
                     EGLYSPWKIRSKIKEAHEIALCSEHGVVEEFIPRPET"
     gene            complement(9039..9926)
                     /locus_tag="U6309_002087"
     CDS             complement(9039..9926)
                     /locus_tag="U6309_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505525.1"
                     /translation="MSFFSTKNTSLQSNFIPEDGLEFSNKSQGMLYYRSDTRSPEQIF
                     KLGFSPRLPEFQNEWWKEAIKSRGYIDDLGIDYRAVDSDPSVCVCMTTKLESAPIFPL
                     SQENCYIYAIALPEFTQIKYLGKGTGDITLSRTGGTPLDPKNIVIDLHNLQSKQVGNI
                     CRFFGSQTKNLGLYAGWPLHAYEALTYRVLPQSIICAIRCQREALDLQIEVDCILGEK
                     LRLCAGKQFTFTGNIIENQHFSMGQLLYVGRPGKSKLCEMDYTLVKERAVAEITNIKN
                     QPEYCFTPNIYYGLGGKTF"
BASE COUNT         3318 a         2053 c         1784 g         3101 t
ORIGIN      
        1 aatgcgtcat ttgagcacat aaacccagtc aaggacgata cttgctaaga caatgcaatg
       61 cacttggtca aaataatctt cacaggcaaa acaaataaag aataattgtt gtgtctcgca
      121 tctatagagc ctccctttaa gcgacaacaa ttgcctatgc caaaagaatc accgaatgca
      181 cacgattaca ttgtatttct cttctaagaa cactagtatt ccaattgtcc tacagcttca
      241 caccaaaaga ttctgattcc gatgttgagc ccccaatgaa ttgatttaat actccagcaa
      301 gcatgctaaa agagccctcc aattgattca agctattcaa taagtcttga aactgactaa
      361 aagaatcttg aaattcattt gcttctttta tcatagattg aggcgtattt tggcttaaat
      421 ctgtgagcaa ttcgataagc tcagcatgtc gcgcgttcag ttttttcagg tttttattct
      481 ttctgcttgc tccctgcgat atttgcatga ttgttgtctg aacgtctatt aattcactgc
      541 aatagctgag cattaatctt tcattgctta aattttccag tacggatttc ttaagctcgc
      601 taaagctaat gtcgatgtta aaatcgattt ctaataattc tttcttcaat tcctgattgg
      661 ctgaattaat tagggtctga aaagcaaagt tctttccggt tttatgataa aatgccttca
      721 ccttacgaac caactcttta tattctttat cagccatggc tttttctctt gatatttcct
      781 catcactctc aattgcttca gccacagcct cagacaatag ttttttggta atatcactta
      841 tttcatcagc caatgcctga ttttgaggac tcagaagtga ggatgagttt tttacattag
      901 cctttaagtg ctcgtatcga gcaatggact cttccagggc aattttctgg tcaccttcaa
      961 tatcatcaga taacaaacat gattttaacg cattaattaa atgagcataa aattgcttgt
     1021 tgttgagggt taaacttttg ttttgctcta acgcggcata taagggatgc ctggggattt
     1081 cagacaaaag aagatgagct aaaggtaatt tatgcaagcc aacaggctgc atcaaacttg
     1141 cgccattttt tattaataca ctaaagcagt ctaccaacga ttcactatgt tctgagaagc
     1201 agtactcaac ggcattggaa tacgttttta ctttggtctt aattggataa ctattgatag
     1261 gcaaaccaac tttagtgact aaaaagctta aaagatccgc ttttctctga gtaagcgcaa
     1321 gcaccattac atcattatta atcaaaggat aataaggcga cagggtttga gcgtattcaa
     1381 actctccagc aaacaaagct ctatccagga gtgacttggc ttctttattt gcacggcttt
     1441 cgatttcatt tagggcttgt gtttgctctt ttgtcagcaa ctcttccgtt tccaaattaa
     1501 tgtctttgat tttccaatgc aaatcaatta aaagaactgc tttaattttt ttatcttccg
     1561 tctttaatag ttgagcaaac aattttttta tcttgctaat atcgcgctcc attttcacat
     1621 ccacagggcg tttcgctttg gctgatttgg aaaataaggt cgattgtgca agcccgatat
     1681 tgcctctgct gattttgggc ttcgtagccc cttgctttgg agacaaggca acaacctctg
     1741 cttggtcatc ttttttctgc tcttcatcac ccttattgac tggctcaaga attggttctg
     1801 gcatagagga tacgtacttg tgtgttttca ccaggtaaat tgcctctctt gaccaatgat
     1861 tactttcaga aatttgactt aatgatttag tcgtctgaat taattcatca agcttttcca
     1921 aatagaccgc tctgttttca tccaaatcct tacttagtag agtcgtttcc tcatttaatc
     1981 tgtggtaatc agcaagcaat ttagcttcta tttccttttg tttttttcgc agcagttgca
     2041 aacaaggcag gcctagctgc catacagaat gcatgaaata ttccagattt tctggtggag
     2101 aaactttgac gtattgctcg tcatcatcca ataactccca agtagctggg catgccagag
     2161 catcaaaacg attcaaaaac aaatgcattc gataggtttg accattttga tcatggaaga
     2221 tcgcagtaaa atgcgaaagt cccaaatttg gattatgggg atctgtcttc tcataaagac
     2281 taatatgcgc gtcatgcagt ctgaaacctt cagcagcaat attagccaag ggtaattcta
     2341 aacgataata cttcttatcc tttaaggtaa tatcatggac aggtctaact tcatctaata
     2401 atttaaaatt tggggattca gctaaaatct gggtcaatac aaagtaaaac aaatttggtt
     2461 tcataatctc aaacagctca ttgaaatgag ccaaggatac ataaggaaac aaatttgatc
     2521 aacatgtttt ttttaaatcc acgcactgtt gtccacaaca tggaatgaca tcctcaataa
     2581 caacaagcaa atgccggtaa gtgccgcatc gattcaataa aaagccaaca gtatgcagtt
     2641 agaaagaatc aggacttacg aaaaacccca agatcaaggc aaaaaatatt tttaatgagg
     2701 gagtttagat aaactaaatg accgaattaa aaattttttt aacgaagaga ttgggatttt
     2761 tcgtaagtcc tgagaatgta aaacttgtgt ttaccggatc cttgcttaac tcattgagca
     2821 ccccaaagtg atagattgta tagcactttg gggaaattga ataagtggct tttcgagaaa
     2881 aaactcattt cattactgta aattatgctt cggtaggaaa atcttcaacc ggaggagcta
     2941 tccatagact gcaatttgga gcaggcatga cataagttgt tacaacgtga gagttcatac
     3001 tattaagcat ggcttttcta gtcgtaagtc cagtattttt aacagtgcaa accatatttt
     3061 tttgtgccag gaacgccgcg caaccattat tttcgggaga attggtgtaa tcaatatctc
     3121 gggttgtcat gagataaacc cgtgttttgt agggtaatga ttcagagagg agtttaaaac
     3181 tgaaaggacc agctatatgg tgttgtcccc atgaagtaat ttgctctgaa gccacggaaa
     3241 tcacttgagg aagtccttgc acattcacag taattgtttc agaactattg ggctcaacag
     3301 gtaaattatt aatggtcagg gaaaaatctt cagccagtac tttaggaaat tccacattaa
     3361 atatagcagc ttgagcagct ggatcacctc ggtgatcagc ctttacactg tcaaaaatat
     3421 gccaaagttt attataagca ttggtacaaa gctgagcatc ttttgaactg ggaagcacac
     3481 ttgcgaaagc tggattgaaa ctggcaacaa ttacggctat aaaaagaaat atatatttca
     3541 tgtaactaac tccttgttat ctaagttgaa ttaaatttga cttgatctga tacaacctat
     3601 tgaaaaaaaa gagttttcat tttgaaaaga actgcgagct taaagcaagc tcaaaaactc
     3661 tcatgataga taatagaata aaaaatatta aatacaaatt tagcttctat atctgtataa
     3721 gccctaagca tagccctgtg tggtacaaac tcattgattt aaaggattaa agcgctcaca
     3781 aacatcaggg tatgaataaa ctataaacaa ttaaaaacgc aacctggacg ccagtcacta
     3841 caaggccatt ttgtatcatg gctttgacat tctcacttcc agcaaaaccc attgccgcga
     3901 acatattggc tccagggtaa gcataatttg tgacgcgagt tgcaaagagc atgcacaaag
     3961 caaaagtgat tagaggcaat tgcatctgga tgacagaggg gctaaagagc tggtgaagca
     4021 ttttgatggt tgcttctgca gcgcctggca taccaaaagc gccggtaaat cctattaaga
     4081 tcgataatac tggtttccct cccaggttaa ttaatggtgt taataattca gttaatgcat
     4141 taaatcctcc ggcttgatgg attagtgcca taaaaggatc aaataaaata aacaaaaaga
     4201 agagatgcaa gcttttttgc attccttcaa ataacaggtt aaaaatttgt ttcaaaccga
     4261 gtcgacagga taatccggta atcagcgcca gaaataacat gacaaagatc acataagagg
     4321 ttttcgcttg agtaaataaa ccatagacaa cacaaaacaa aaaggaaatc agaaaaatta
     4381 atgtagccct tttttgttgt ctattgggag taaaaggctc agtgctgttc tcgtcttcgc
     4441 agggctcatt gatgtatagt ttttgtaatc gtgaagccat aaaccaggtg gtaattaaag
     4501 tcactacagc aattggaaga gaagcgtaaa gaaatactgt cccataactt aatccagtga
     4561 cgccaagcaa ggtcacaacc gggggggcaa atggccctag aattaaagcc tcttcagctg
     4621 aggcctgcat gagtaccgcc aaactgggac gtgacaagcc tactgaccct gcaataggac
     4681 gcaaactggg agccagaatg gctaaacctc ccgctaatgt tccaagcaaa ccaacaataa
     4741 taaaggatga gaggactata ccaattttag cacgtcgctg agtattaacc ccaactccat
     4801 aaacaatttg gtgcactaaa gtatgactta cctgggtatg cgttaaaacc tcgcccaacc
     4861 ctcgacctaa catgataata aaaccaacca aagccataaa tgaacccaag gcgtcagcca
     4921 tagcattccc tatgtcaatg ggagtgctta tattccacaa aaaacctagc ccaacgcaaa
     4981 ctgctgtcac tactaatgga tcaacgttac gaaataacag taaaacataa agaataataa
     5041 tagataaact ggctagctga atgtagaaag acattagcgg atcaccccaa ttagatttag
     5101 tccttgtgtg atgccttcaa tccctaatcc aggtttctct gccaggataa ttttattgct
     5161 gagactttgt gccccaccaa gaatataatt ctcgcggatc aaaaaaatag gatcgagatc
     5221 tgcataagaa atatcgggct tacttaatgc aaaactggct attgcagcaa ctccaatcgg
     5281 cgactccagc atacatccaa ccattatttg cattttggcc gcttgagcaa tatggtagat
     5341 agattgagct ttctcaatgc ctcctgattt catcaacttt atattgaccc catcacaagc
     5401 attaatcttt gcgatattca atgcatcttc tggtgaaaaa cacgcttcgt cagcgataat
     5461 tgcacaatca acctgatcac taatcgtctt taaatcaggc agatttcgag cactaaccgg
     5521 ttgctccacc agaggaacat tcaaattctc ttttttgaac gcagtaatgg cgattaatgc
     5581 ctcttcataa gaccatccct gatttgcatc tgctagcaga gtaatgtcat tgcctactgc
     5641 ctgacgaata gctcgaattc gattaatgtc gtccactgga tttaatccca atttgatttt
     5701 cagggttttg tgccctcgct gaattaaatc aatcgcatca cccaccatct cctcaacttc
     5761 tttaacactt atcgtaatac atgagttgat gcaattggta tttcctccca attgtttata
     5821 aagaggcaag ccacaatatt gggcaaataa atcatgcaaa gcaatatcaa tcgcagcttt
     5881 ggctgaggaa ttacctccta tagcccggtc attcatttca agtaataaat tgaatgcaga
     5941 aatatcacgt ccgataagag gaggccccaa ggcgttttta atagcagcaa taatggattc
     6001 cttgctatcc ccagttattg caggtgttga tgcagctgaa ccataaccaa tctttccaca
     6061 atccgttttt attaatatca cgacatcatc aacgcattct gtacgcctca ccgctgtaat
     6121 aaacggacgg cttaatggaa tagttagttc tgcgatatgg atttctttaa tattcaaaca
     6181 agcacctgtt catttatagg ttgcagggga tcataacaat ctcaattttt tacccaattt
     6241 tattatgcat tgttctgctt ttagaaacca atataatcaa ttgacaacag ccatatacct
     6301 tgattgataa ttgatagctt atcgttattc atgcctgcca aaacacccct tatctgtatt
     6361 tatatactga ttaacttgta aaatatcaac taattggcct tatcggagta ctacccaatt
     6421 cattctgctc aaaatatttt aaagtacagt atttcaaatt aaaaaacatc tgagaatcac
     6481 tctgaattaa ggctatatga ctaatgatca ttacaacata atcacttgtt cctgctcaat
     6541 tttcattcct caaaaattac ataagatata cagaggccaa aaaacaacct tggaaaatcg
     6601 gtgtttttta tactagtatt taaagagaat aagcattatt tttagatatg tatgaactga
     6661 atgacacgcc ctattttata tacagcaatt taatacagga atattgttcc cctctcttta
     6721 atctgacacc tattaaaggg tttacttttt gtcgcacata tggagataaa acggcttttt
     6781 tattaaccag tgaatatcaa ttccatatag aatgcatcca acacgaaaag aaaatttcca
     6841 aacttaatat gttgcgtaca cctgagtata ctcaagccgg ctattttctt aactcccctg
     6901 ctcttgatgg aaaatactct cccttttacg aaagtaccgc tcaaaaatat ggttatggat
     6961 ttagctttac tattgtcagg aaaaatgaga atcactgcga ttattttcat tttataggga
     7021 acaggcatca aaaagatatg aataattatt atcttcataa taaatggtta ctggataagt
     7081 tttgcgacta ttttcttgaa cgggcagcga aagtattagc ttacagtgag aaagaaaata
     7141 gattcacgct aaccaataca gcagaacatc cttttcctct taaaattcat tcaagtgcgt
     7201 tagatcaatt tttaaaggac tatcattttg aacaatttgg acttaaacca gtttgcttgc
     7261 ctaaacgaca aattgaatgt gcgcagttat tagtccgtgg aaaaacagca gaggaaatag
     7321 ctattattct taacttatca aagcgcactg ttgaacacta tattaacatc ttaaaaagaa
     7381 aactaaatgc ccataacaaa ggactcctta ttacaaaatt gcttcaccag tatcctgaca
     7441 tgtttgaatc aattctctag gtagaggtaa tgttctgtat gtaaaaatct catgggggag
     7501 gagatgactc gagaaatttc actggctcca aagtctcaga ggtgacaaaa aactgggatg
     7561 tatccctcgc ctcaagatct tcaggataat tttttaactg ttttacttgg caaaatgtgt
     7621 cttcttttac tctgaaaaga gcaaaaccac attgtctccg gcgcatgact tcgcggattt
     7681 tattcattct ttcatttgaa taaatgacat taggagaata agctaaaatc aaatcatcat
     7741 tttgtacagg atgcttggta taagtcatta ctttataacc cttttcaaga aaagtaagcc
     7801 cttgcccatt tctactgaaa atcttctccg cttctttctc caataatacc ttagctgact
     7861 ttgttatatt ttttcttaaa aaaaaactgg tcatatttta ctctcttctg acaatgacaa
     7921 cctactaatt attaccgaga aaaaatttaa agaaataccg taaaaatacg ggttaaaaaa
     7981 tccggtccca taatcttgat ttcattttaa ttctttatcc ttgcacaagg gataaagaag
     8041 tttgtataca gggagtaaaa ttagctggag attaatgata tgttttttaa aacctgtgaa
     8101 ttccaaagaa acaaatcaag cgatactgta tacccaagga ttgatatacg ccaagccatc
     8161 tacaaggaaa gtataagttt aacccctgaa atggcaatat caatgcagaa aaaaggaggt
     8221 tatcaaacag ataatgattg gaaaaaaatg ctaatgggag acgttaccca aaatcttctc
     8281 attcaatcag ttattgggaa aaaaagcctg atttgtgccc cccctaacat taactccatg
     8341 tccttggagg atttccatct ctggactaaa cagccacttc ccgctggaac cagtcagtac
     8401 cttgcgctac ccaatgcatt cgcggataat ctgatacatg gatatcattt tgtcgaggca
     8461 aggaactatc accatttagt ccaggttgga gaagtaaaat gccaaaaaac tggaaatacc
     8521 ttgtacccat taagaaaagc attagccgta tttgcgattt ctgagtggat gcaggaacta
     8581 cctgttcttg ttgccagagc gtcagccaat accgaacatg gcgaaggtgg cgcgattcaa
     8641 ttgattgtac ccctggaatt attgcgcctc cttcattcca atgtaaaact actgattact
     8701 caagaagaca atgaatattc agtggagaaa aaagaggggt tatactctcc ctggaaaatc
     8761 cgatctaaaa taaaagaggc gcatgaaatt gcactctgtt cagaacacgg tgtagtcgag
     8821 gaatttatcc cacgaccaga aacctaatga ggagttatga tggccaagga tgagcccttt
     8881 tattatctgt aaaaacatta tcttactggc atactccaaa attcttaatt cacaacttat
     8941 cctttgggta ttgattgcaa ttttaattga ttctacctaa agccagcttt attaatgtat
     9001 tgctatccac acaattattc ccagcaaaat cactttggtt agaaagtctt acccccaagt
     9061 ccataataaa tattaggggt aaaacagtat tcaggctgat tttttatatt agtaatttcc
     9121 gctaccgccc tttccttgac caatgtgtaa tccatctcgc acaatttgga ctttcctggt
     9181 ctgccaacat ataagagctg ccccatggaa aaatgttgat tctctataat gtttcctgtg
     9241 aaagtaaatt gtttgcctgc acataacctg agtttctctc cgagtataca atcgacctca
     9301 atttgtaaat cgagtgcttc tcgctgacat cgaatggcac aaatgataga ttggggtaac
     9361 actctgtatg tcaaagcctc atacgcatgt aatggccatc cagcatataa accaagattt
     9421 tttgtttggc ttccaaaaaa cctgcaaata ttacccactt gtttgctttg caaattatgc
     9481 aaatcaatca caatattttt agggtccaaa ggggttcccc cggtacggga taatgtaata
     9541 tcgccagttc ctttacccag gtatttaatc tgagtgaact cagggagcgc aatcgcgtaa
     9601 atatagcaat tctcctgact tagaggaaaa atcggagcac tctccagctt ggtagtcatg
     9661 caaacacata ctgaaggatc cgaatcgact gctctataat ctattcctaa atcatcaata
     9721 taacctctgg atttaatcgc ttctttccac cattcatttt gaaattcagg caatctggga
     9781 gaaaaaccca gtttaaaaat ttgttctgga gaacgagtat cagaacgata ataaagcatc
     9841 ccttgacttt tattggagaa ttccaggcca tcttcaggaa taaaatttga ttgtaatgaa
     9901 gtattttttg ttgagaaaaa actcattctg cctccatttg ctgacaagct caataatgcg
     9961 tgaaccaatt attcccaaca ggcactaaaa ataaatacag aagaaatact gtatttaaaa
    10021 aaatgtatct tttggtatca tccaatattt ataaaatctg ttcataaagt aaaaaaattc
    10081 ggtcatttta ttttcaaatt tatcctggag atactcatgc ttttcagatc caacaaatcc
    10141 agcgcccaca aacctgaaca aattagcaga aattaccacc gtttgatgct gcagttgaaa
    10201 acatgggaaa caaatcacta cacaccagac ttaaatgaag aatattttag gacttt
//
LOCUS       DASLOE010000110        10208 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.111, whole genome shotgun sequence.
ACCESSION   DASLOE010000110 DASLOE010000000
VERSION     DASLOE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10208
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.111"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..788)
                     /locus_tag="U6309_002088"
     CDS             complement(<1..788)
                     /locus_tag="U6309_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN5505526.1"
                     /translation="MTIQSIATSDFQGLNELKIQAKNNAKEALPEVAKQFEGIFLQSM
                     LKSMRMGQHFLDESSPFSGENEATFQEMLDAQYASTIAESKGIGLAALLAKQLENSVG
                     YKANSLDNSSAEVNNTKVTNSEESISVVDDFVKSVWPTAKKAASLIGLDPKLLMAQAA
                     LETGWGKFVTRDADGSSSNNLFNIKTGSHSEVESIQVKTTEYIADTPIKINASFRKYP
                     SIEHSFHDYVSLIRGSERYQMALANAANPEIYVSELNKAGYATDP"
     gene            complement(800..1903)
                     /locus_tag="U6309_002089"
     CDS             complement(800..1903)
                     /locus_tag="U6309_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEN5505527.1"
                     /translation="MRRILVIRWILAIHLIATQVFAERIKDIATLAGVRVNQLVGYGL
                     VVGLSGTGDKTGTKFTEDSFANMLTQLGINIPPGVRLNSKNIAAVMVTANLSSFMKKG
                     QTMDVNISSIGDSKSLLGGTLLLTPLKGADGRVYAMSQGNIVVSGISASGSDGSSVTV
                     NVPSGGRIPNGATIEADIPNPFYYSNSLTYNLHTPDFTTAKRMSDAINELMGPGTAKA
                     IDAGSVVVTAPKKLSQRVDYVSVLENIEFKPGEPMAKIIINARTGTVVISSNVIVKSA
                     AVSHGNLVVSITETPVISQPNAFASGRTVATQQSQVNIQQKNNRAFILPKGTTLKDIV
                     RGINAVGATPADVISILEALQQAGALSATLIVI"
     gene            complement(1942..2634)
                     /locus_tag="U6309_002090"
     CDS             complement(1942..2634)
                     /locus_tag="U6309_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEN5505528.1"
                     /translation="MNRLNIAVSCLATALLFGCEALHPPAPGDNPDYAPTYPVTPDPK
                     ELRKVSGAIYSSETALPLFETPRARHPGDILTVFLIEKTDAQKNATTTQRKNDTTKIT
                     NKLFLGRPISLGSGYSMDFDLDNQRQFNGEGRSIQNNKLAGSISVTVAKVLANGNMVV
                     QGEKWVRINQGNEFVRLSGIVRPQDIKADNTITSDRIANARISYGGTGQINNTNAQGW
                     LSRILWGPLFPT"
     gene            complement(2647..3432)
                     /gene="flgG"
                     /locus_tag="U6309_002091"
     CDS             complement(2647..3432)
                     /gene="flgG"
                     /locus_tag="U6309_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEN5505529.1"
                     /translation="MEPALWVSKSGLEAQDKNIATIANNLANVNTTAFKKGRALFEDL
                     IYQNLRQAGAQSSQNSQIPTGINMGTGVHLVATEKIFSQGSLQNTQNALDVAIEGRGF
                     FTVLMPDGTQAYTRDGSFKTDSTGQIVNSNGYPLQPNITIPPQTTKITIGTDGTVSAL
                     VAGNNAPSVIGNIQLTDFINPAGLQPIGQNLYTETVASGTPITDNPGNSGLGTLLQGS
                     LEASNVNVVEELVNMIQAQRSYEITAKSIQTVDSMLQYLTQTL"
     gene            complement(3583..4329)
                     /gene="flgF"
                     /locus_tag="U6309_002092"
     CDS             complement(3583..4329)
                     /gene="flgF"
                     /locus_tag="U6309_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEN5505530.1"
                     /translation="MEPILYKAVSGGRSDFKRQEMIANNLANINTPGFKADLYQAQTM
                     YMNNANGNNQFNMHSFITQNTNGVDTSPGEIITTGRDLDVAIDGDGWMAVQDSQGREA
                     YTRGGSLRLNANGQLITASGKVVLGDGGPISIPPAQSIEIGSDGTISIVPLEGDVKSL
                     AILDRIKLVTLDRNNIYKNDEGLIQLRSGAATTNSNIKLQSGALEGSNVNAIDQMVAM
                     ISAGREFDAQMKLLSTVDDNGQKLAQLLQE"
     gene            complement(4340..5653)
                     /gene="flgE"
                     /locus_tag="U6309_002093"
     CDS             complement(4340..5653)
                     /gene="flgE"
                     /locus_tag="U6309_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEN5505531.1"
                     /translation="MVFNTALSGIQASTRDLEVIGNNIANSATIGFKGSRAEFADIYS
                     SSAYGSGGNAVGGGVKLSRVHQSFATGTLSTSNNTLDLAVNGSGFFILSDQGAKVYTR
                     AGQFKLNNENYIVNANNQRLTGLLTDSNGAITGASGDLRINTANIPPRASSNVSVGVN
                     LSSQSKAPNVDWVGGASPATDTYNNVTSSTIYDSLGNSHVLSMYFIHADSTALAGSPN
                     AASPAGTENQWYVAFQIDNQNVPPNVGAQNTNNLFRANFNSDGSFAGVFDTSNAALPN
                     NLIPLSFNLNNGSNPLSLSIDLSDSTQFGSPFAVQSTFNNGYTTGSLAGLDIDDSGVI
                     YGRYTNGQSLSMGQIQLANFADPESLQNLGNTNWAETTSSGQPLISAPGSGGLGLINS
                     GRLEESNVDLTSELVNLISAQRNFQANAQTIRTGDAITQTIINIR"
     gene            complement(5766..6443)
                     /gene="flgD"
                     /locus_tag="U6309_002094"
     CDS             complement(5766..6443)
                     /gene="flgD"
                     /locus_tag="U6309_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEN5505532.1"
                     /translation="MTTNSVNGSNSIGGLGLNQIDSDKRQNLGQQDFLRLMVAQVQNQ
                     DPLQPQANGEFLSQLAQFSTNDGITKMQESLQQLASSLQSNQALQASALVGRKVLVNS
                     NTLSLGTEGDVKAAIDMAPGVSNLRAAIYTESGELIKTIPLGQPEPGFFQFSWDGTDQ
                     SNQRLASGRYTIKVTGTYGGQEVALKTMTSANVDSVSLGQNGEDLKLNVAGVGSVYLS
                     QVRQISV"
     gene            complement(6454..6876)
                     /gene="flgC"
                     /locus_tag="U6309_002095"
     CDS             complement(6454..6876)
                     /gene="flgC"
                     /locus_tag="U6309_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEN5505533.1"
                     /translation="MSLNAIFDIAASAMTAETVRLTTSAGNMSNANVEAGSPDEVYQA
                     KYPVFKSVQENANQWIGEQIKAGVKLDGIYQSDAEPHRRYDPNSPIADKDGFVYTSNI
                     NYVEEMANIISASRSYQMNIELLNTTKQLMQRTLQLGE"
     gene            complement(6879..7271)
                     /gene="flgB"
                     /locus_tag="U6309_002096"
     CDS             complement(6879..7271)
                     /gene="flgB"
                     /locus_tag="U6309_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEN5505534.1"
                     /translation="MAINLDSYFGIHAKALIARDQRASVLANNIANVNTPNFKARDVD
                     FNEVLTATMAGNSQQLRVTSDQHISTNADFMTHLKYRMTHHASLDGNTVDKDMETTEF
                     ARNALNYQASLSFLNGKIKSMLTALKGE"
     gene            7487..8422
                     /gene="hemF"
                     /locus_tag="U6309_002097"
     CDS             7487..8422
                     /gene="hemF"
                     /locus_tag="U6309_002097"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEN5505535.1"
                     /translation="MSISKTLPYNAIEQIKSYLLQLQNTICVLLESIDGKTRFHEDSW
                     QRAAGGGGRTRIMTNGNVFEKAGVNFSHVSGEQLPASASAHREELAGRHFSALGVSLV
                     IHPQNPYVPTTHANVRFFVAEKEDSEPVWWFGGGFDLTPYYGFVEDCEHWHQTALNAC
                     LPFGETIYPKFKRWCDDYFFIKHRNEARGIGGLFFDDYNEISFDHSFELMRSIGDHFI
                     LAYEPIVARRKDIPFGNREKAFQNYRRGRYAEFNLVYDRGTLFGLQSGGRTESILMSL
                     PPTVHWEYNWHPEKGSDEEKLYTDFLPAKDWLKKE"
     gene            complement(8567..9874)
                     /locus_tag="U6309_002098"
     CDS             complement(8567..9874)
                     /locus_tag="U6309_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5505536.1"
                     /translation="MTTDFIGLLKQRKFLPLFLTQFFGAFNDNAYKLAMLTMISYHLS
                     HTQEQSEYYQAIAGALFILPFFIFSATSGQLADKYDKALLTRLVKVLEVLLMIIGGIA
                     LYSGSIFLMMCTLTGMGVHSSFFGPIKYAILPDHLPKKNLLAATGLIEASTFIAILLG
                     TTLGTLTVGGKEAIPYIAIIMTLSAAFSGLVSSLFIPPAPSALHELKVDFNLWRATVK
                     MLKEAKKEYGILISILTISWFWLIGAVMLTKLPDYTHYVLGASAHVFALFLALFSIGI
                     ALGSITINWLLKGQVNLSAVPMAMLAFTFFAFDLYWSSPAGHDLKTPLFNLFNFFTSF
                     THWRIAFDLFMLAFCAGLFVVPLYTYLQIISPVENRARTIAANNIFNALFMVMGTLLV
                     MLLLFFSFSIPQVFLILCLLNLVAAVFLFLGLRTLRRSSEEIA"
BASE COUNT         3073 a         2190 c         1860 g         3085 t
ORIGIN      
        1 gggtcagtag cataccctgc tttatttagc tcactgacat aaatctcagg atttgctgca
       61 ttggccaagg ccatctggta tcgctcactt cctctaatta atgaaacata gtcatgaaaa
      121 ctatgttcta tggatggata ttttctaaag gaagcgttga tcttaatggg tgtatcggca
      181 atgtattctg ttgttttaac ctgaattgat tctacttcgc tatgcgaccc ggtttttata
      241 ttaaataaat tattactgct cgaaccatct gcatctcttg taacaaattt accccagcca
      301 gtctctaatg ccgcttgcgc catcaacagc ttgggatcca atccaattaa tgatgcagcc
      361 ttttttgctg tgggccacac agattttacg aaatcgtcaa caacagatat cgattcttct
      421 gaattagtca ctttagtatt gttaacctct gcagaagagt tatccaggct gtttgcttta
      481 tacccaactg aattttccag ctgtttagcc agtagagccg ccaatcctat ccctttagac
      541 tcggcaatag tactagcata ttgtgcatcc agcatctctt gaaaagttgc ttcattctcg
      601 ccactaaaag gactcgattc atcaaggaaa tgctgcccca tcctcatgct ttttaacatg
      661 gattgcaaga atattccttc aaactgttta gctacctcag gcaacgcttc ttttgcattg
      721 ttttttgcct gaatttttaa ttcatttaac ccttgaaaat cactcgtagc aatactttgt
      781 atggtcatat ccatcacctt tatatgacta ttaatgtagc actcaatgcg cctgcctgtt
      841 gtaaagcttc cagtatggaa atcacatcag caggtgttgc accaacagca ttgattcctc
      901 tgactatatc tttcaaagtg gtacccttgg gtaagataaa ggcacgatta tttttttgct
      961 gaatattgac ttgtgattgc tgcgtagcca ccgtgcgacc ggaagcaaag gcattaggtt
     1021 gactgataac gggagtctct gtaatgctaa cgaccaaatt accatgagat acggcagcag
     1081 atttaacaat cacattgctt gaaataacca ctgttccagt cctggcattg ataataattt
     1141 ttgccatggg ttctcctggt ttaaattcaa tattctcaag tactgaaaca taatctacgc
     1201 gttgagataa ttttttaggg gctgtgacta ccacagaccc agcatctata gcttttgctg
     1261 tgccaggccc cattaactca ttgatagcat cactcattcg tttcgctgta gtgaaatcgg
     1321 gggtatgcag gttgtaagtc agtgagtttg agtaataaaa tggattggga atatcagctt
     1381 caatcgtagc gccattggga attcttcccc cgctgggtac gttaacagta acactggaac
     1441 catcgctccc ggaagcgctg attccagaga ctacaatgtt tccctgtgac atggcataaa
     1501 cacgaccatc cgctcctttc aaaggagtaa gtaataaagt tccacctaat aaacttttag
     1561 aatcgcctat ggacgaaatg ttgacatcca tagtctgtcc ttttttcata aaactagaca
     1621 aattggcagt aaccatcact gctgcaatgt ttttggaatt gagtctaact cctggaggaa
     1681 tgttaattcc caattgagtc agcatgttag caaaactgtc ttcggtaaat tttgtgcctg
     1741 tcttatcccc agtgccgctt aaaccaacca ccaacccata cccaaccaat tggttaacac
     1801 gaacaccagc caaagtagcg atgtctttga ttcgttcagc aaaaacttgg gttgcaatca
     1861 agtgaattgc caatatccat cgaataacca gtattcgccg catcttaatt ccttgtcgtt
     1921 aataatctcg ataattacca attaagtcgg gaataaaggc ccccataaaa tccttgataa
     1981 ccatccttga gcattcgtat tattaatttg accagttcca ccataagaaa ttctcgcatt
     2041 ggcaattcta tctgaggtaa tagtgttatc tgcttttata tcctggggac gaactatccc
     2101 tgaaagacga acgaattcat tcccctggtt aattctgacc cacttctcgc cttgaacaac
     2161 catattgcca ttcgccaaca ctttggctac cgtcactgaa atactgccag ccaatttatt
     2221 gttttgtatt gatctgcctt ctccgttaaa ctgcctttgg ttatccaaat caaaatccat
     2281 gctgtaacca cttcccaaag agatgggtct acccaaaaag agtttatttg tgatttttgt
     2341 cgtatcattt tttctctgag tggtagtcgc atttttctga gcatcagttt tttcgattaa
     2401 aaacactgtt aatatatcac ccggatgcct tgccctaggt gtttcaaaca aagggagagc
     2461 cgtttcacta ctataaatag caccacttac ctttcttaat tccttgggat ccggagttac
     2521 aggataagtt ggagcataat cagggttgtc gcctggtgcc ggtgggtgta aggcttcaca
     2581 cccaaacaac aaagctgtag ctaaacaaga gacagctata tttaatcgat tcataaccta
     2641 tctcacttac agagtttggg ttaaatattg caacatgctg tcaacggtct gaatgctttt
     2701 tgctgttatt tcataacttc tttgagcctg aatcatatta accaattctt ctaccacatt
     2761 cacattagaa gcttctaatg aaccttgcaa taaagtacct aatccggagt tgccagggtt
     2821 atctgtaatc ggtgtaccgc ttgctacagt ttccgtatat aaattctgcc caatgggttg
     2881 caaccccgct gggtttataa aatcggttaa ttggatattt cctataacgg atggcgcatt
     2941 atttcctgcg actaaagcac taaccgtgcc atctgttcca atagtaattt ttgttgtttg
     3001 tggcggtatc gtaatgttgg gctgaagagg atacccatta ctattaacaa tctggcctgt
     3061 actatcagtt ttaaagcttc catcacgtgt ataggcctgt gtgccatctg gcattaagac
     3121 tgtaaaaaag ccacggcctt cgatagcgac atccaacgca ttttgtgtat tctgaagact
     3181 gccctggctg aatatttttt ctgtagcaac cagatgcact cctgtcccca tgtttatacc
     3241 ggtaggaatt tgcgaatttt gtgaggattg agcaccagcc tggcgaagat tttgataaat
     3301 taaatcctcg aataaagctc tacctttttt aaaagcagtt gtgttgacgt tagcaagatt
     3361 attcgcaata gtagcaatat tcttatcctg ggcttccaaa cctgatttac tcacccataa
     3421 tgctggttcc ataacggcct cctgctatta ttcttattat attatattgg ccttaattat
     3481 catttcagtc tatcaacacc ttgcgctcct gaaaccatct atagttccta atcattggaa
     3541 agcggcttcc cctaactgaa ataataatta aattaattaa gtttattctt gcaatagctg
     3601 tgccaatttt tgtccattat catcgactgt tgataataac ttcatttgag catcaaattc
     3661 acgaccagca ctaatcatag ctaccatttg atcaatcgca tttacattac tcccctccaa
     3721 tgcaccgctt tgcaatttaa tattactatt agtggtagcc gcacctgatc tcagttggat
     3781 caaaccttca tcatttttat agatattatt tctatccagc gtaactaact taatcctgtc
     3841 taagattgcc agagatttga catcgccttc taaagggaca atcgatatag ttccgtccga
     3901 accaatctct atggattgag caggcgggat agaaatggga cctccatcgc ccaaaacaac
     3961 cttacccgag gcagtaatta actgtccatt agcattcaaa cgcaaactac cccctctggt
     4021 ataagcttca cgaccttggc tgtcctgaac agccatccac ccgtctccat caattgctac
     4081 atccaaatct ctgcctgtcg taataatttc cccaggagaa gtgtccactc cattggtatt
     4141 ttgagttata aatgaatgca tattgaactg attatttcca tttgcattat tcatgtacat
     4201 ggtctgtgcc tggtacaaat cggccttaaa tccaggggtg ttaatatttg ccaaattatt
     4261 ggcgatcatt tcctgccttt taaaatctga acgaccgcca gaaacagcct tatacaggat
     4321 aggctccata gcttacctcc tagcgaatat tgatgatcgt ttgagtaatc gcatcgcctg
     4381 tacgaatggt ttgagcattc gcttgaaaat tgcgctgggc actaattaag ttaaccaatt
     4441 cacttgtcaa atccacattt gattcttcaa gtcttcctga attaattaat cccaaaccac
     4501 cacttccagg agcgctaatc aatggctgtc ctgatgaagt tgtttcagcc cagttagtat
     4561 ttcctaaatt ttgcaaactt tcaggatctg caaaattagc gagctgaatc tggcccatgg
     4621 acaaagattg tccattagta taacgaccat agatcactcc cgagtcatca atatccagac
     4681 cagcaagact tccggtagtg tagccattat taaaagtcga ttgaacagca aaaggactgc
     4741 caaattgagt actatctgat aaatcaatac tgagacttaa aggattggaa ccattgttta
     4801 aattgaaaga taggggaatt aaattattgg gtaatgcagc gtttgatgta tcaaatactc
     4861 ccgcaaacga gccatcagaa ttaaagttag ctcggaataa attatttgta ttttgagctc
     4921 caacattagg aggaacgttt tgattgtcta tctgaaatgc tacataccat tgattttctg
     4981 ttccagcagg tgatgcggca ttgggtgaac cggctagggc tgttgaatcg gcatgaataa
     5041 aatacatgct caatacatgt gagtttccta aactgtcata gattgttgac gaggtgacat
     5101 tgttataagt atcggttgcc ggactagccc ctccgaccca atctacattc ggcgctttgc
     5161 tttgagagct cagattcaca cctacagaga catttgagct ggctctggga ggaatatttg
     5221 cggtgtttat tcttaaatca cctgatgcgc ctgtaatagc accattagaa tcagttagta
     5281 aaccagtcaa tcgttgatta tttgcattga ctatatagtt ctcattattc aacttgaatt
     5341 gcccagcccg agtataaacc ttggctccct gatcacttaa tataaagaaa cccgaaccat
     5401 tcacagccag atcgagcgta ttgtttgagg tacttaatgt tcctgtagcg aatgactgat
     5461 gtacacgcga gagtttaacg ccaccaccta cagcgttgcc cccagaacca taagcgctgg
     5521 atgaataaat atctgcaaac tcggctcttg agcctttaaa tcctattgtg gcagagtttg
     5581 caatattatt acctataact tccaaatctc tggttgatgc ctgaattcca cttaatgctg
     5641 tattaaaaac cataatagcc tcctgcttta taatttttag catttgccct tgactgcttt
     5701 ataagcaatg cgcttatgac ttaagccatc gtttcaataa ggccacggtg actgaaatca
     5761 ttgatttata ctgatatttg ccttacctga cttaaataga ctgatcctac gcccgctaca
     5821 ttaagcttta gatcctcgcc attttgccca agactgacac tatcgacatt ggctgacgtc
     5881 atagttttca atgccacttc ttgtccgcca taggtaccag taacctttat tgtatatctt
     5941 cctgatgcta aacgttgatt actttggtca gtaccatccc aactaaattg gaaaaacccg
     6001 ggctctggtt gccccaaggg aatagttttt atcaactcgc cagattccgt gtagattgca
     6061 gcacgaagat tagaaacacc aggtgccata tcaatggcag cctttacatc tccttctgta
     6121 cccagactca gggtattgct gttgactaac accttgcggc caaccaaagc ggatgcttga
     6181 agcgcttgat tagattgtag agatgaagct aattgttgca gtgattcttg catttttgtg
     6241 attccatcat tggtgctaaa ctgagccagt tgagataaaa actcgccatt tgcctgtggt
     6301 tgcagaggat cttgattttg aacttgtgcc accatcaaac gcaaaaaatc ctgttgtccc
     6361 aagttttgtc ttttatctga atcaatttga ttcaaaccta aaccacctat tgaattggaa
     6421 ccatttactg aattggtagt cattcccttc cccttattcg cctaattgca gcgtcctttg
     6481 cataagttgt ttggttgtat taagtaattc aatattcatt tgataagaac gagaagctga
     6541 aatgatattt gccatttctt caacgtaatt gatatttgac gtatagacaa acccgtcttt
     6601 atcagctatt ggactatttg gatcgtacct tctgtgcggt tctgcatcac tctgataaat
     6661 accatccagt ttcacgccag ccttaatttg ctccccaatc cattgatttg cattctcctg
     6721 gactgacttg aaaacaggat atttggcttg atagacctca tctggactac ctgcttcaac
     6781 attcgcatta ctcatattgc cagcactggt agtcaaacgc actgtttctg cggtcattgc
     6841 agaagccgcg atgtcaaaaa ttgcatttaa tgacatgatt actccccttt caatgcggtc
     6901 agcatggact tgattttgcc atttaagaaa ctcaagctgg cctgataatt caatgcattt
     6961 cgagcaaact cggtagtttc catatcttta tcaacggtat taccatctaa agaagcatga
     7021 tgtgtcattc ggtatttcaa atgagtcata aaatcggcat tggtactaat atgctggtcc
     7081 gaagttaccc ttaactgctg gctgttaccg gccatagttg ctgtcagtac ttcattaaaa
     7141 tcaacatccc gggctttaaa attcggtgta ttaacattgg ctatgttatt agcaagaact
     7201 gatgccctct gatctcttgc aatcaacgcc ttggcatgaa tcccaaaata agaatcaaga
     7261 ttaatggcca tatttactcc tgtcttattg ttatttaagt cttaatcatc atttgacatt
     7321 atgccaattt tccaaagcac tatatggatt catacttgtt tccaaacttc actgaaacat
     7381 aaaaatgata aaaaaatgct atttaaacaa agcaaacatt gtgccaatat ccaaaaaatg
     7441 agaaaatgaa acatccaagc gctaattccc tatgtggagt cccatcatgt caatctcaaa
     7501 gacacttcct tataatgcga tagagcaaat taaatcttat ctgctccaac tccaaaatac
     7561 aatttgtgtc ttactggagt caattgatgg caaaaccaga ttccatgagg attcctggca
     7621 gcgagctgct ggaggtggag gaagaacaag aataatgacg aatgggaatg tctttgaaaa
     7681 agccggcgtc aatttctcac atgtttcagg ggaacaatta ccagcatcag ccagtgcaca
     7741 tagagaagaa ttggcaggaa ggcatttcag cgcattaggg gtatctctgg tcattcatcc
     7801 tcaaaacccc tatgtgccaa caacacatgc caatgttcga ttttttgtcg ctgaaaagga
     7861 agattcggaa ccagtctggt ggtttggtgg aggatttgat ttaactcctt attatggatt
     7921 tgttgaagac tgtgagcatt ggcatcaaac ggcattaaat gcctgtttac cttttggaga
     7981 aacgatttac ccaaaattca agcgttggtg cgatgattat ttttttatta agcacagaaa
     8041 tgaagcacgt ggaattggcg gtttattttt tgatgattac aatgaaatca gctttgatca
     8101 cagctttgag ctcatgcgta gcataggcga tcattttatt ctggcttatg aacccatagt
     8161 ggctcgccgt aaagacatcc cttttggcaa cagggaaaaa gcatttcaga attaccgcag
     8221 aggccgttat gcggagttca atttagtcta tgatcgaggc actttatttg gtctgcaatc
     8281 tggcggaagg actgagtcta tattgatgtc cctgcctcct actgttcatt gggaatacaa
     8341 ttggcatcct gaaaaaggca gtgatgaaga aaaattatac acagattttt tacctgcaaa
     8401 agattggttg aaaaaggagt aaaacaccaa attcaaaaat aaatgatttc cttctcccct
     8461 tacctgaaaa tttcgaagag ccagtataca tgcagtaaat gagcaccgcg aaacgtttac
     8521 aacgcgcaat aaaatgaaag agacaggtta tgcaagagat ctgttgttaa gctatctcct
     8581 ctgagcttct tcttaaggtt cttaacccaa gaaacaagaa caccgcggca accaaattta
     8641 aaagacaaag tatcaaaaaa acctgaggaa ttgaaaagct aaaaaacaat aacagcatga
     8701 ctaacaaagt tcccatcacc ataaacaagg cattgaaaat attgttcgcc gcaatcgttc
     8761 tagccctgtt ctcaactgga cttataattt gcaagtaagt atacaaggga actacaaata
     8821 gtcctgcaca aaatgctaac ataaataaat caaaagcaat tcgccaatga gtaaatgaag
     8881 taaaaaaatt aaataagtta aataaggggg tctttaaatc atgacccgca ggagatgacc
     8941 aatacaaatc aaaagcaaaa aaagtaaaag cgagcatggc cattgggaca gcagaaagat
     9001 taacttgtcc tttcaataac caatttatag taattgatcc cagagcaatg cctattgaaa
     9061 ataaagcaag aaatagagca aaaacatgag cgctagcccc caaaacataa tgggtataat
     9121 caggtaattt ggtaagcatg acagcgccaa ttaaccaaaa ccaggaaata gttaatatgg
     9181 atataagaat tccatattct tttttcgcct cctttaacat cttcactgtg gcccgccata
     9241 aattaaaatc cacttttaat tcatgaagtg ctgatggtgc aggaggtata aacaggctgg
     9301 agaccaaacc agaaaaagcg gcacttaagg tcataataat cgcgatataa gggatagcct
     9361 ccttccctcc aacggttaac gtacccaatg tagtgcctaa taaaatagca ataaaagtgc
     9421 tggcttcaat taggcctgtc gctgctaata aatttttctt aggcaaatgg tctggtaaaa
     9481 tcgcatattt gataggtcca aagaaagaag aatgtacccc catccctgtc aaagtacaca
     9541 tcatcaagaa aatgctacca ctgtataatg caatacctcc aataatcatt aataacactt
     9601 caagcacctt aaccagacga gtcaacaaag ccttgtcata tttatctgcc aattgtccgg
     9661 aagtagcgga aaagataaag aaaggcagaa tgaataaagc gcctgcaata gcctgataat
     9721 attccgactg ttcttgtgta tggcttaaat gatagctaat catagtcaac atcgctagct
     9781 tataagcatt atcgttaaaa gcaccaaaaa attgggtcag gaaaagaggc aaaaattttc
     9841 tttgtttgag aagaccgata aaatctgttg tcattaaagt tccatttgcg ctttaaaact
     9901 aaagttaaca aggatattgc tgtattgatc gaaagtaaat gacaaaagcc tgagaaaaat
     9961 tcagaaaata ttatccacac aaagaccaat tgtatatatt caataaaata taaatagaaa
    10021 ctgacttgga acgcaaatac tcagtaccta ttacccagtc cctcgtacat caatactacc
    10081 gacctattgc ctcaatgaaa gatgttgccg aagaatcttg ctatctaatc taattaggaa
    10141 aactcactta actggtctat atttacatat aggagtttat ttttatttat gttgctctaa
    10201 ttaatgca
//
LOCUS       DASLOE010000111         9969 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.112, whole genome shotgun sequence.
ACCESSION   DASLOE010000111 DASLOE010000000
VERSION     DASLOE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9969
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.112"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            258..1301
                     /locus_tag="U6309_002099"
     CDS             258..1301
                     /locus_tag="U6309_002099"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEN5505537.1"
                     /translation="MSYSILKKLPPIEKIIQELPLPHPAYLQIAQDRQEVKAILEGRD
                     KRLLMIVGPCSAWPKKAVLEYAERLVQLNSKVKHALKLIMRVYIQKPRTTKGWTGPVN
                     QPDLFSTPDIEAGIKYTRDMMIKVIQMGLPIADEALFTHNAKGFLELLSWVAIGARSS
                     EDQEHRIFASALDCAVGLKNPTHGSLAVGVNSLVASQHPHVAVFDGYEVQTHGNHHAH
                     LVLRGSNQAPNYSVSHLEEVMYYMGMHQIVNPSVIIDVSHDNCLVNGKKNHQLQPEIA
                     FNILESIKNRPDLKQLVKGFMVESFIKEGNQKLDSINPDNIDLSGLSVTDPCLSWEKT
                     ESFLLELAEREAL"
     gene            1298..1882
                     /locus_tag="U6309_002100"
     CDS             1298..1882
                     /locus_tag="U6309_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEN5505538.1"
                     /translation="MNNLVEKKEAFRFGVSGDIGSFSEEAALLYAKKKGLNAHLVHLL
                     DMEGVLNAIENETIELGIFPVVNLLGGLVKPAFQAMGKYSFTPIDEIWHDINQCLLVK
                     SSTQHHKITHIVSHPQGFAQCKKFLGNKFKNAKKIEWIDTAKAAKDLAEGILPPHAAV
                     IASARCAPLYDLKISARNIQDSHLNRTAFILVKK"
     gene            1916..2176
                     /locus_tag="U6309_002101"
     CDS             1916..2176
                     /locus_tag="U6309_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEN5505539.1"
                     /translation="MGTIEELRKQIEQTDADIIEKLAQRQELAKQIGALKAKEGKKII
                     DRLREKKLFEYYDFLSRQYHLQQDFVNQLFKIIISNSKKVQK"
     gene            2176..3342
                     /locus_tag="U6309_002102"
     CDS             2176..3342
                     /locus_tag="U6309_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN5505540.1"
                     /translation="MNHYEKMSLLKDSATIAINSLAQQKINAGIKIHNLSAGEPKLPP
                     HPSIINAVTQAMEQGHTLYPPIMGIPELRHMGCEWMYRSYDCSYQNEQCLVVNGGKLG
                     IYLLMQLLLQPNDEVIIPSPYWVSYPAMVSLFGGTPVPVETTEAEEWKLTPQALQKAC
                     TTKSKILILNNATNPTGALYTQSELINLLQIAKKHNLLVISDEVYSELTYDGHHYVSC
                     GSLPQFRERVIIIQSCSKNFSMTGWRVGFIFAPEIFIKPLSALVSQSTSGVTTLSQWA
                     AVAALQEVNQVNIWVQQNMEKRRNCLLRALHDYLGLTITPPVSSLYVFLSLKDIGCKN
                     QNSEEFCKQALDKANVAMVPGIAFGKEGYVRLSFGGNEDDLKSGIYALAQWLHR"
     gene            3654..4550
                     /gene="tehB"
                     /locus_tag="U6309_002103"
     CDS             3654..4550
                     /gene="tehB"
                     /locus_tag="U6309_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEN5505541.1"
                     /translation="MPREYDNLICYKQLSINDHGKLKFFLEKHSTKEGTWGKLSLNEG
                     EIDFIFQNGLGQELSRIRLDKNNRQLSIPPASWHKIIPISASFDAQLGFYCKPHRYFN
                     KKYGLTEVHRDLLYVYHTYLSHLGSLTTLDVGCGSGRNLLYLAQLGHTVTGIDINQTA
                     LENIQIIAQKENLSPLNILSQDLNQPQSLAPDHYDFVCSTVTLQFLNASRIPSLLIEL
                     QEATKKNGYHFLVFPVQSELYSLPSSFTFLPKQEELYHFYQDSGWSILEYKESVGHLH
                     KQDELGRPVQGLFALLLAQKVI"
     gene            complement(4583..5014)
                     /locus_tag="U6309_002104"
     CDS             complement(4583..5014)
                     /locus_tag="U6309_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEN5505542.1"
                     /translation="MQLTQFTDYSLRALIYIALKKGSCTIKDITDAYSISNNHLIKII
                     HNLSKMGLIKTIRGKNGGILMAASPETINLGQLIMELEPNFDLVPCFNKEKANCCIAP
                     ACKLKSVLYEAQRAFLQILEQYTLADVLHNPSELSVLLRIS"
     gene            5094..5468
                     /locus_tag="U6309_002105"
     CDS             5094..5468
                     /locus_tag="U6309_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEN5505543.1"
                     /translation="MTESLFERLGGQNAVNTAVDIFYRKMLMDDRVNYFFDDVDMEQQ
                     ILKQKGFLTMVFGGPNQYTGKSMREGHQHLLARGLNDSHVDIVIEHLGETLKELGANE
                     EDIQKVAAIANSVRGDVLGRSS"
     gene            5465..6421
                     /locus_tag="U6309_002106"
     CDS             5465..6421
                     /locus_tag="U6309_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEN5505544.1"
                     /translation="MNTVRFNNQSFALTPDESILQCFLRHGVEYPNSCQAGICQSCLI
                     KAKDGEINPDWQEGLPETLKSQGYFLACLAKPSTSLYVASPDNAECETQAQIMSLQRL
                     NHNVIQVKLKVEHPAPWLPGQYLSLINPEGTMRNYSIANIPADDGYIELHIKIYPQGK
                     MGQWLLHRAAINTFITIRGPFGQCYYHNPHNLAFDILLAGTGTGLAPLIGIIRSALTQ
                     KHEGTITLVHGGVTDEDIYYKEELEMLSLLFSNFRYDPCVLQSQGLYPEGSIEKRVLT
                     HLHSPNTTKVYVCGPKETTNKLKTRIFLAGVPSTNIYSDVFL"
     gene            6646..7665
                     /locus_tag="U6309_002107"
     CDS             6646..7665
                     /locus_tag="U6309_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEN5505545.1"
                     /translation="MIVDFETHFITEACIDYLTQRQEVPKLVPELNGAYTMCFTPDVS
                     LFHTSALMEELLSLNEQRLAIMDQAGVTIQVLSLTTINGIDSCPGDENKSTALAREVN
                     DHLYSAIQKHPERFKGFASISPYDVKEGVKELERAISQLGFVGWLTHSNFGEDNYLDD
                     KTYWPLLEAAEGLNIPIYLHPNVPIMREFGKYGFALGGSALGFEFDTALCLMRMILGG
                     VFDAFPKLKIMLGHLGETMPFLMERLDHLYRIPDLKPYRPSIQRIPSEVLRQNVYITT
                     SGRFFVPALRYVLEVMGEDRVLFASDYPMESLLDATRFIQDSDLSNQTKQKIFSINAK
                     NLNLI"
     gene            complement(7788..9203)
                     /locus_tag="U6309_002108"
     CDS             complement(7788..9203)
                     /locus_tag="U6309_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2538 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505546.1"
                     /translation="MIPVPVMRKTHELVMIQAAAGFFMKTKYEMSQDKTEFLAKEQDS
                     SYPGYQVSVLDLEKIVKHYQEKYGIRLIINGTTPKYQSLIKERQVNFEQQKQQFLELK
                     YAKFLQIFFQPPNLNGANSPFSINKHMGAFIGFYEEIYNKVLPFLDAKGKIISGLSLE
                     ELRQLNEACQELSCKGILDAKINEFIERNFDYMGLTARESASEIKDICDELQEGEVLG
                     YFFTGQRTSGRCHFDLYICLPGKAIRPIFYNTALIRYHDLGGMFHLNFPFVEGSFFTP
                     DLLKLYSAMDLQQLIPQADRTSCGTLTMMYAKELLKDDARGLKEFTLSFTYYNEKGEK
                     EYFFLPSPQVLRYSQISLYNEALKAIVSHENDGQAGLVRIGAKKYMFHTVEKILIQSF
                     KIALEKEDADVLEENQKIWDMLPSFQEKWQESYKEMVAKRAVMHQGVNKYLLYSTHRM
                     SHIASEQSINNEADADRIILR"
     gene            complement(9786..>9969)
                     /locus_tag="U6309_002109"
     CDS             complement(9786..>9969)
                     /locus_tag="U6309_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lpg2539 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505547.1"
                     /translation="YQLEDPCHLTWEEFTEKQCLLADAEASRILLWQANKKASKPEIE
                     KDLMSVASSNAMNPDM"
BASE COUNT         3062 a         2051 c         1901 g         2955 t
ORIGIN      
        1 ctccaaatca gttaaataaa aaaacaagcc ttgcacaggc ttttgcttgc tttttttatt
       61 ttagcttttt cagccctgag ctgggtggta ggtaggcaat actaacactc caacccccag
      121 ctgagtctgg gggtttttga tacccaagat ccccagtcta ataggggggt tgggtacaaa
      181 agaggagtgt gaacgatgaa ccatcaatgt caaattcatc atccatctaa agcgctttct
      241 ggcttgagga ggatgcgatg agttattcca tcttaaaaaa actgccacca atagaaaaaa
      301 tcatccagga actgccacta ccccaccccg cctatctaca aattgctcaa gaccggcaag
      361 aggtcaaagc tattttggaa gggcgagata aacgtctttt gatgatagtt ggtccttgtt
      421 cagcatggcc taaaaaagcg gtcttggaat acgctgagcg tttggtacaa ctcaacagca
      481 aagtaaaaca cgctttaaaa ttaatcatgc gcgtttacat tcaaaagcca agaaccacca
      541 aaggctggac tggaccggtg aatcaacccg atttattttc aactcctgat attgaggcag
      601 gtataaaata tacccgggac atgatgataa aagtcattca aatggggctc cctatcgcgg
      661 atgaagctct tttcacccat aatgccaaag gatttcttga acttctgtcc tgggttgcaa
      721 taggcgcccg tagttcagag gatcaagagc atcgaatttt tgcctccgcc cttgactgtg
      781 ccgtaggatt aaaaaaccct acacatggtt cactcgctgt cggagtaaac agcctggttg
      841 cgtcacagca tcctcatgtc gctgtcttcg atggctatga agtgcaaacc catggtaatc
      901 atcatgctca tctcgttctg cgtggttcaa accaagctcc caattactcc gtttctcatc
      961 tggaggaagt catgtactac atgggcatgc atcagatagt gaacccttct gtcattattg
     1021 atgttagcca tgacaactgt ctggtgaatg ggaaaaaaaa tcatcagcta caaccagaga
     1081 ttgcatttaa tattctggaa agcatcaaaa atagacctga tttaaaacaa ctggtcaagg
     1141 gattcatggt agaaagtttc atcaaagaag gtaatcaaaa attagattct atcaatcccg
     1201 ataacattga tttaagcggg ttatccgtta ctgatccttg cctcagttgg gagaagacag
     1261 aatcgttcct gttggagctg gcggagaggg aagcattatg aataatctcg ttgaaaaaaa
     1321 agaagccttt cgctttggtg tttcagggga tattggatca ttttctgagg aagcggcttt
     1381 gctttatgcc aagaaaaaag gccttaatgc ccatctcgtt catttgctgg atatggaagg
     1441 cgttttaaat gccattgaga atgaaacgat agagctgggc atattccccg ttgttaacct
     1501 tttaggagga ttggtaaagc cagcatttca ggctatgggc aaatattcat ttactcccat
     1561 cgatgagatt tggcatgaca ttaaccaatg cctgctggtt aaatcgagta ctcaacatca
     1621 taaaatcaca catattgtgt cacatcctca aggctttgcc caatgcaaaa aattcctggg
     1681 aaacaaattt aagaatgcga aaaaaataga atggatcgat acagcaaaag cagcaaagga
     1741 tttggcagaa ggaatattgc caccccatgc cgcagtcatt gcgtcggcac gttgtgcacc
     1801 actttatgat ttaaaaatca gtgcgagaaa tattcaggac agccatctga acagaacggc
     1861 ttttatatta gttaaaaaat aagtgataag gttcctatcc gctaaggagt tcatcatggg
     1921 tacgattgaa gaactaagaa aacaaattga gcaaactgat gcagacatca ttgaaaagtt
     1981 ggctcaacgc caagagttgg ccaagcaaat cggtgcttta aaggctaaag aaggaaaaaa
     2041 aataatagat cgcctgcgcg aaaaaaaatt gtttgaatat tatgactttt tatccaggca
     2101 atatcacttg cagcaggatt tcgtgaatca attattcaag ataattatct caaactcaaa
     2161 aaaggtccaa aaataatgaa ccattatgaa aaaatgagct tgctaaaaga ttccgcaaca
     2221 attgccatca attctctagc ccaacaaaaa ataaacgcgg gaatcaaaat tcataatctc
     2281 agtgctggag aacccaaact gccaccccat ccctccataa taaatgccgt cacacaagct
     2341 atggaacaag gacataccct ttatcctcca ataatgggga ttcctgaact acgtcatatg
     2401 ggctgtgaat ggatgtatag atcctatgat tgctcatacc aaaatgagca atgcctggtt
     2461 gtgaatggcg gaaaacttgg catttattta ctcatgcaat tattactgca gcctaacgac
     2521 gaagtcatta ttccttctcc ctactgggtt tcctatccag ccatggttag cctgtttggc
     2581 ggcacccccg tgccagttga aacgacagaa gccgaagaat ggaaactaac accacaggca
     2641 ctgcaaaaag cgtgtaccac aaaaagtaaa atactcattc tgaataatgc caccaatccc
     2701 accggagctc tgtataccca gtctgagctg attaacttat tgcaaattgc caagaagcat
     2761 aatcttttag tgatctctga tgaagtttac agcgaactga cctatgatgg gcatcattac
     2821 gtatcctgtg gatctttacc ccagtttcgg gagcgtgtca tcatcattca aagctgttca
     2881 aaaaactttt ccatgaccgg gtggcgagtg ggttttattt ttgccccaga aatattcatc
     2941 aaacctttaa gcgccctggt cagtcaaagc accagtggag tgaccaccct tagccaatgg
     3001 gctgctgtgg ctgcgctgca agaagtaaat caagtcaata tctgggttca acaaaacatg
     3061 gaaaaaagaa gaaattgcct cctccgcgcc ttgcatgact atctgggatt gactataaca
     3121 cctccagttt cctcacttta tgtttttctt tccctaaagg atattggctg caaaaatcaa
     3181 aattccgagg agttttgtaa acaagctttg gataaggcaa atgttgcgat ggtgccggga
     3241 atcgcctttg gtaaagaggg ttacgtccgc ttgtcttttg gaggcaatga agacgattta
     3301 aaatcaggta tttatgccct ggcgcaatgg ctgcatcgct gattaaagta attcattact
     3361 ttgcaagaag cattgctaga cctcttgcct aatctgatta ttttcaggac ttacgaaaaa
     3421 ctccaaggtc aaggcaaaaa atgtttttaa taagggagtt tagataaact aaatgaccga
     3481 attaaaaaca ttttttaaca aagagatggg ggtttttcgt aagtcctgat tttattttac
     3541 tgcagttttg cagttaataa cctgctccat ataacggctc tataaaattg atgcattcga
     3601 caataggcta aaaaaccatt attttattta tcaatacatc aggataaaaa accatgccaa
     3661 gagaatacga taatttaatc tgctacaaac aactatccat taatgaccat ggaaaactta
     3721 aattttttct tgaaaagcac agcactaaag aaggaacatg gggcaaactt tccctaaacg
     3781 aaggtgaaat tgattttatt ttccagaatg gcttgggtca ggaactgtcg cgtattcgtc
     3841 ttgataaaaa taataggcaa ctatctattc ctccggcctc ctggcataaa atcattccga
     3901 tcagcgcatc gttcgatgct cagcttggtt tttattgcaa acctcatcgc tactttaaca
     3961 aaaaatacgg cttaactgag gtgcaccgtg acttattgta tgtgtatcat acctacttgt
     4021 cgcatttagg ctcactaacc actctggatg tcggttgtgg ttccggtaga aatctgttgt
     4081 atttggccca acttggccat acagtgactg gaattgatat caatcaaacg gcactggaaa
     4141 atattcaaat aatcgctcaa aaagaaaacc tgagtccact taatattttg tcgcaggatt
     4201 taaatcaacc tcaatccctt gcaccagatc attacgattt tgtctgctct accgtgacgc
     4261 tccaattttt aaatgcctca cgtatcccct ctttattgat tgagttgcag gaggcaacga
     4321 agaaaaatgg ctatcatttt ctggtttttc ccgtccaatc tgaactttat tcactcccaa
     4381 gctcattcac gtttttacca aaacaagaag agctgtatca tttttaccag gacagcggct
     4441 ggtctatcct cgaatacaaa gaatcagtag gacatcttca taaacaggat gaattgggaa
     4501 gaccagtgca aggattattt gctctgttgc ttgcccagaa agttatataa agatcacttt
     4561 cataaaatat tactctggat tgctaactga tcctcaataa aacagacaat tcacttggat
     4621 tatgtaaaac atcagccaga gtatactgct ctaaaatctg caggaaagca cgttgcgctt
     4681 catacaaaac gctcttgagt ttacatgctg gggcaataca gcagtttgcc ttttctttgt
     4741 taaaacaggg aaccaaatcg aaattgggct caagctccat aatcaattgg cctaaattaa
     4801 tggtttcagg tgaggccgcc atcaaaatac cgccgttttt cccccgtatt gtcttaatta
     4861 aacccatctt cgataaatta tgaatgattt tgatgagatg gttgtttgat atggaatagg
     4921 catctgtaat gtccttaatg gtacatgagc ctttctttaa ggcaatgtag atcaaagcac
     4981 gcagggaata atcagtaaat tgagtcaatt gcataaaaaa tccttctggt ttgttgacgt
     5041 ctattatttc tttttataag atgtatttca aatgcatctt taaggagtat atcatgactg
     5101 aatcattgtt tgagcgatta ggtggccaaa atgccgtcaa cacggctgtg gatattttct
     5161 accgcaaaat gctcatggat gacagggtga attatttttt tgatgacgtc gatatggagc
     5221 aacaaatcct taagcaaaaa ggatttttaa ccatggtgtt tggcgggcca aatcaataca
     5281 ctggaaaaag tatgcgggaa ggacatcagc atctacttgc cagaggctta aatgactcac
     5341 atgtggatat tgtaatcgag catttagggg aaacccttaa ggaattgggc gccaatgaag
     5401 aagacattca gaaagtagct gcaatcgcca atagtgttcg tggcgatgta ttggggcgct
     5461 catcatgaat accgtacgat ttaacaatca atcttttgca ttaactccgg atgaaagcat
     5521 cttgcaatgt tttttgcgcc acggggtaga ataccctaac tcctgccagg caggcatttg
     5581 ccagtcatgc ctgattaaag ccaaagacgg agagattaat ccagactggc aagaaggatt
     5641 acccgaaaca ctcaaatctc aaggatattt cctggcttgt cttgccaaac caagtacttc
     5701 gctttatgtc gcatctcctg acaatgccga atgcgagaca caggctcaaa taatgagcct
     5761 gcaacgctta aatcacaatg ttattcaagt caaactgaag gtggaacatc ctgccccatg
     5821 gctacccgga caatacctgt cgctcattaa tcctgaagga acaatgcgca attattccat
     5881 agccaatatc cctgctgatg atggatacat tgaactgcac atcaaaattt acccgcaagg
     5941 caagatggga caatggcttt tgcatcgcgc agccattaat acattcatca ccatccgagg
     6001 cccttttggt caatgttatt atcataatcc acataatctg gcttttgata tattgctggc
     6061 agggacaggg actggcctcg ctccgctgat aggtatcatc agaagcgcat taacacaaaa
     6121 gcatgaagga acaattaccc tggtgcatgg cggggttact gatgaggata tctattacaa
     6181 ggaagagctg gaaatgttgt ctttattgtt cagcaacttc cgctatgatc cctgtgtgct
     6241 gcaaagtcaa gggctttatc cagaaggctc aattgaaaaa cgggtgctaa ctcacttgca
     6301 ctctcccaac actacgaaag tgtatgtgtg tggtccaaag gaaacaacca ataaattgaa
     6361 aacacggatt tttcttgcgg gtgttccttc aacaaatatt tatagtgatg tttttttata
     6421 atatttaaat agccctggtg atttataatc tggtgcaaac cagattatgt ttctcgctca
     6481 tcaaaatgat ctcttctttc cgctgatagc atttcttttc tttaagacat tttccggcaa
     6541 tttgccatca gaacttttat agccatgtgg cgatatctaa tcttccggct atacttaagc
     6601 atataatttt caatcgtttt tctcacgtct aaaaggatta cgttcatgat cgtcgatttt
     6661 gaaacgcact tcatcactga agcttgtatt gattatctga ctcaacgcca ggaagttccc
     6721 aaattggttc cagagctcaa tggagcttat acaatgtgtt ttacacctga tgtttcctta
     6781 tttcacacaa gtgcattaat ggaagaatta ttaagtttaa acgagcaacg actggcaatt
     6841 atggaccaag caggggtgac aatccaggta ttaagtttga ctactatcaa tgggattgac
     6901 tcttgtcctg gagatgaaaa caaaagcacc gctttagcaa gagaagtcaa tgaccatctt
     6961 tactctgcca ttcaaaaaca tcctgaacga tttaaaggtt ttgcttctat atcgccttat
     7021 gacgtcaaag aaggtgtaaa agaattggaa agggcgatat ctcaattagg ttttgtaggt
     7081 tggcttacac actccaattt tggcgaggat aattacctgg acgataaaac ctattggcca
     7141 ttgctggaag ccgcggaagg tttaaacatt cctatctacc tgcatccgaa tgtacccatc
     7201 atgcgcgaat tcggtaaata tggttttgcc ttggggggaa gtgctttagg gtttgaattt
     7261 gacacggctt tatgtctgat gcgaatgatt cttggaggtg tattcgatgc gtttcccaaa
     7321 ttaaaaatta tgctggggca cttgggtgaa accatgcctt ttttaatgga gcgtctcgat
     7381 catttatacc gtataccaga cttgaaacct tatcgacctt ctattcagcg tattccctca
     7441 gaggtattac gtcaaaatgt ttacataacc actagcggac gtttcttcgt tcccgcctta
     7501 cgttatgttc tagaggtcat gggagaggac agggtattgt ttgcctccga ttacccaatg
     7561 gaatccttac ttgacgctac tcgatttatt caagattccg atttatcaaa ccaaacgaag
     7621 caaaaaattt tctctataaa tgccaagaat ttgaacttaa tatgaatcat atctcttgcg
     7681 tcatatcagt aaggagctga ttttcttgca gacattaatt gagccaggct caattaatgt
     7741 catgctttct agaaataaaa tacaaacagt ttcattctgc aatattttta acgcagaatt
     7801 atcctatcag catccgcttc attgtttatg gattgctcac ttgcaatatg gctcatccga
     7861 tgggtggaat aaagcaaata tttatttacc ccttgatgca tcacagcgcg tttggcaacc
     7921 atttccttat aggattcctg ccatttttcc tgaaagctgg gtaacatatc ccaaattttt
     7981 tgattttcct ccagaacatc ggcatcttct ttctcaagtg caatcttaaa agattggatg
     8041 agaatttttt cgacagtatg aaacatatat tttttagcgc ctatcctgac caaaccagcc
     8101 tgtccatcat tttcatgaga tacaatggct ttcagcgctt cattatataa acttatttga
     8161 gaatatcgta atacttgtgg tgatgggaga aaaaaatatt ccttttcacc tttctcatta
     8221 taataagtaa aactcaacgt aaattctttc agccctctcg catcatcttt taataactcc
     8281 ttggcataca tcattgtcaa agtaccacat gaagtcctgt cagcttgagg gattagctgt
     8341 tgcaagtcca ttgcagaata taattttaac aaatcaggtg taaaaaaact gccttcgaca
     8401 aaaggaaaat tcaaatgaaa catccctcca agatcatgat acctgataag cgctgtgtta
     8461 taaaatatcg gtcttatggc cttaccaggt aaacagatat agagatcgaa atgacatctc
     8521 ccactggtac gttgaccagt aaagaaataa cccaaaacct ctccttcttg cagttcatca
     8581 cagatgtctt ttatttcact tgcgctttcc ctggcagtta accccatata atcaaaattt
     8641 cgctctataa attcattaat ttttgcatca agtattcctt tgcatgacag ctcttggcat
     8701 gcctcattga gttgtcttaa ctcttccagg gataatccag aaatgatttt tccttttgca
     8761 tccagaaatg gaagaacttt attgtatatt tcttcgtaaa aacctataaa tgcacccata
     8821 tgtttattga tagaaaaagg agagtttgcg ccattcagat taggtggttg aaaaaatatt
     8881 tgtaaaaatt tggcgtattt gagttccagg aattgctgtt tttgttgttc aaaattaacc
     8941 tgacgctctt ttatcagaga ctgatatttg ggtgtagtgc cattaataat caagcgaata
     9001 ccgtattttt cctgataatg cttaactatt ttttcgagat ccagaacgct gacttgatac
     9061 cctggataag aactatcttg ttctttagcc aaaaactcag ttttatcttg actcatttca
     9121 tattttgttt tcataaaaaa cccggctgca gcttgtatca ttactaattc atgagttttt
     9181 ctcatcaccg gaactggaat caattaattg cccaccagga tgattttcat tgatttcata
     9241 atgacaactc cttgctctat ctcaatgaaa caactgatta taaaaatcat tattttttca
     9301 ttatgaatcc cttataaatt aacaaaccct ctgcctaata aagactgaca gagaccatgg
     9361 atatttcaat ttagagacaa aaagaaagga aacgacgatt tgttaagtaa cgcttcgctg
     9421 aattaaagtt aataaactac tctcaatcac caaatccgag agtaaatcgc tcatcttctt
     9481 tgcctcagcg acgaacccgt ttacttaatt aaagctaagt gttaccacta gtcctccgta
     9541 tatccttgtg gagaagatag gccgcagcaa aaagatcatt ttttatgatc ttttggtgag
     9601 caaaatttta acaataaaag cttaagaatt tattaaatgg gcaacacctt atattgaggc
     9661 cataggggac tcaagacctt tcactggtta actgattgga tgtatttaat ttttttcatt
     9721 gcatgactca agtaatgaat tacaaaacga acactcacta tgtaatacag gacattttat
     9781 tcttgttaca tatcaggatt catagcatta ctgcttgcaa cggacataag atctttttca
     9841 atttcaggtt tacttgcttt cttattagct tgccaaagaa ggattctact ggcctctgca
     9901 tccgctaaaa gacactgctt ctcagtaaac tcctcccagg tcaaatgaca tggatcttca
     9961 agctgataa
//
LOCUS       DASLOE010000112         9945 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.113, whole genome shotgun sequence.
ACCESSION   DASLOE010000112 DASLOE010000000
VERSION     DASLOE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9945
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.113"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..688
                     /gene="rpoN"
                     /locus_tag="U6309_002110"
     CDS             <1..688
                     /gene="rpoN"
                     /locus_tag="U6309_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEN5505548.1"
                     /translation="QVIHKLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLNQDIL
                     PRLSINSQYAALIQRANNSLDNQFLKHNLQEARWFLKSIQSRQETLLKVATCIMEYQH
                     GFLEFGEEAMKPLILNDVASALDMHESTVSRVTTQKFINTPRGVFELKYFFSSHVNTD
                     SGGECSSTAIRAVIKKLISAENRKKPLSDSKIAQLIGEQGIKVARRTVAKYREAMGIP
                     PSNERKVISS"
     gene            715..1014
                     /gene="hpf"
                     /locus_tag="U6309_002111"
     CDS             715..1014
                     /gene="hpf"
                     /locus_tag="U6309_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HEN5505549.1"
                     /translation="MQINITGHGMDVTPALRAFTEEKFDKLERHFDHIRAINVVFDVE
                     KVRQIAEATVLVTKGELHASSESEDMYAAIDTLVDKLNRQLIKHKEKLLAHRDRD"
     gene            1205..1474
                     /locus_tag="U6309_002112"
     CDS             1205..1474
                     /locus_tag="U6309_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEN5505550.1"
                     /translation="MIKTKIKIINRLGLHARASAKFVSTAAKFQSHIDVTKDTQTVNG
                     KSIMGVMMLAANKGSELTLEIDGPDEERMNEALVELINNRFGESE"
     gene            complement(1716..2453)
                     /gene="pssA"
                     /locus_tag="U6309_002113"
     CDS             complement(1716..2453)
                     /gene="pssA"
                     /locus_tag="U6309_002113"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEN5505551.1"
                     /translation="MNKENHSGIYLLPNLLTTVSLFAAFYSIVASTKGQYEAAIVAMF
                     IGMIADSLDGRIARLTNTQTEFGAQYDSLSDMVTFGVAPSLLLYNLTLHQLGKFGWLV
                     AFIYTAAVALRLARFNTQIGKSDKRYFQGLPCPPAAAVMASFAWICFQNEWANLIVSL
                     FTAVLALIVSVLMVSNLRYYSFKEVDYKGKVPFLYVLVMILLFVAIAANPAIVLFASS
                     GIYALSGPMLTVISLHKMRKQRRNMSK"
     gene            2683..2967
                     /locus_tag="U6309_002114"
     CDS             2683..2967
                     /locus_tag="U6309_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HEN5505552.1"
                     /translation="MSNRKNKTIVIEGVTSQGKTFRPSDWAERMSGSLAVFKNSRIYY
                     SPLLQPSVNSEGYKCVLLDPKLKESSPQVYQAIMDFAKANNLKICGEEDL"
     gene            complement(3111..3653)
                     /gene="dotV"
                     /locus_tag="U6309_002115"
     CDS             complement(3111..3653)
                     /gene="dotV"
                     /locus_tag="U6309_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein DotV"
                     /protein_id="HEN5505553.1"
                     /translation="MNTIDLITILGNISQSLYPVQKLITGGAYILGVLFFLTAIGKLK
                     KIGDHRAQSSSQEKMFTPMMYLLVGALLIYLPSGMDLMANTAFGVGNVLTYSNYNPAN
                     IYSSMGFLIRTAGVLWFIRGCVLVAHSSQPGTQQGLKGLVFLIAGVFAMNFDNTIAMI
                     NSALDYFVKATLSVKAAAGF"
     gene            3737..4471
                     /locus_tag="U6309_002116"
     CDS             3737..4471
                     /locus_tag="U6309_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEN5505554.1"
                     /translation="MQINTFPITLEESFMISPKVKHFIFNCELSPPFEYLPGQFITIH
                     FEHDGKNLKRSYSIANEPKKDNRIEFAAGYFEGGPGTELLYNLKPGDVIHINGPFGRL
                     IFKDETPGRYILVATSTGTTPYRAMLNELGQRIEKHPDLQVVILQGVQRSEEILYPDD
                     FQAFAKKYPQASFRPYLSRVQKQDLKDNEYSGYVQHAFPELNLNPTRDMVYLCGNPGM
                     IDEAFNYLKEHGFSMQHIVREKYISR"
     gene            4621..5631
                     /locus_tag="U6309_002117"
     CDS             4621..5631
                     /locus_tag="U6309_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class I"
                     /protein_id="HEN5505555.1"
                     /translation="MSYEELSNTMEQMLHEGKGILAADESNSTIGKRFASIHTENTEE
                     NRRDYRLLLATTPDLEQYINGVILFEETFTQTDEHGTPIPELFANKGIIPGIKVDKGL
                     VDLANTEHEKVTQGLDGLTERLLHFKKLGAKFAKWRNVYSISEYTPSLTAIKTGAENL
                     GRYAAACQSVGIVPIVEPEVLMDGNHSIEHCAEVCEMVLHELFHTLFIHQVELEHIIL
                     KPSMVTCGKEVTPFSDPEEVADYTISVFRNNVPASVPTINFLSGGQTPQQATANLNAI
                     NQIGHQPWLLSFSFGRALQEDCLKEWCGNKENIAKAQEALIKRARLNSMACLGEYQND
                     ME"
     gene            complement(5810..6565)
                     /locus_tag="U6309_002118"
     CDS             complement(5810..6565)
                     /locus_tag="U6309_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEN5505556.1"
                     /translation="MQPEVRSVSLITYNIHKGFGVGAVRFLLPEMRDAIAGLNPDFVF
                     LQEVQGKHRKREKRIVSWPDSLQCEYIAENIWPHYVYAKNAVYQSGHHGNAILSKYPF
                     ESFESINLSNMNRASRGILHTQLKLDNNIIHLLCVHLGLFKAERTEQCKALIQRIKDV
                     VPKNEPLIMAGDFNDWRHVISKILAENLGIEEAFVALEGQHARSFPAIKPALCVDRVY
                     FRGMKVQEVACLQGKPWRMLSDHLPLYARFELL"
     gene            7032..7880
                     /locus_tag="U6309_002119"
     CDS             7032..7880
                     /locus_tag="U6309_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN5505557.1"
                     /translation="MKTKILSIPGLSIACKAWGNPDNPPILALHGWLDNANSFDDIAE
                     HLQNDYYFIAVDLPGHGHSSHLPPGCIYHFTDGIFTVVEIINALGLNKLHLLGHSMGA
                     CLGSLVAGVAPDRFLSLSLIEGLGPFSHPAETACQQLSKYLDYLSQKQSKKAKGYNKF
                     EHAALARSVKGYVSLDIAKSLCERGIQQENGLYYWRHDRRLLAPSPLQMTETQVLSCL
                     TEIKAQTYLIWASKGFSFDSDKMKARIQAVKNIKIERLDGGHHIHMEKPEVISRLLAK
                     FYQSIK"
     gene            complement(7882..9513)
                     /locus_tag="U6309_002120"
     CDS             complement(7882..9513)
                     /locus_tag="U6309_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEN5505558.1"
                     /translation="MHPNYYLSPLAVAIALGIASPVKAADPIPLQKSSFSEVTQKFQL
                     TLPGVMKGAVVSTNSLQFIRQHTDGNKVTHVRMQQQYAGFPVFGGYAILHSKNATPSL
                     ATAKSDVKMNGVIYDGLQTELGQPKPSFVKNASLALQQFKDKYANKQISEDQVTPMIY
                     IDEKHQAHWAYKVSVLVIHDDRIPERPTAIIDAETNKPFVQWDDVKTEKVQAKGMGFG
                     GNRKIGEYQFGKDLPLLEITRDSSVEMCFMENTDVKVVDMGHKYYSNNKPMQFTCKET
                     PDTQSTKTYYTGYSADGYDRDNGAASPTNDALYAGYVIKHMYHDWYGVEALTKSDGSP
                     MQLVMRVHYGQGYENAYWDGKQMTFGDGDTMMYPLVSLGVGGHEISHGFTEQHSGLEY
                     FGQSGGMNESFSDMAAQAAEYYSVGKNSWQIGPEIMKEDSGYDALRYMDKPSRDGMSI
                     DVADDYYGGLDVHYSSGVYNHLFYILANQPNWNLRMAFDVMVKANMDYWTPYSTFDEG
                     GCGMLSAAKDLGYNLDDVKKSLSEVTINYQSCYVD"
BASE COUNT         3324 a         1947 c         1719 g         2955 t
ORIGIN      
        1 acaagtcatt cataaattaa atccaaaacc aggaagtttg atccatcaag ataatattga
       61 atatgttatt cccgacttaa ctgttaaaaa gatagataat caatggaaag tttttttgaa
      121 tcaagatatt cttcccaggc tgagtatcaa tagccaatat gccgctttaa tccaacgcgc
      181 aaacaatagt ctggataatc aatttttaaa gcataattta caagaagcac gttggttttt
      241 aaaaagcatt caaagccgtc aggaaacact tctaaaagta gcaacctgta tcatggaata
      301 ccaacatgga tttctggaat ttggtgaaga agcgatgaaa ccattaatat tgaatgatgt
      361 agcttctgcc ttggatatgc atgaatcaac ggtatcaaga gttacaactc aaaaatttat
      421 taacacccct cgaggtgttt ttgagttaaa gtacttcttc tccagtcatg tcaatactga
      481 tagcggagga gaatgctctt ccaccgcaat ccgagcggta ataaaaaaat taatttcagc
      541 agagaataga aaaaaacctt tgagtgatag taaaattgcc caactaatag gtgagcaagg
      601 cattaaagta gcaagacgca ctgttgccaa atatcgcgaa gcaatgggga ttcccccatc
      661 caatgaaaga aaggttatta gtagttaatt aatcggtcaa cgaaaggagg ttttatgcaa
      721 atcaacatta ctggacatgg tatggatgtc actcctgcac taagagcatt cactgaagaa
      781 aaatttgata agcttgagcg tcattttgat cacatcagag caattaatgt agtatttgat
      841 gtcgaaaaag taagacaaat agcagaagcg accgtattgg ttaccaaagg tgagttgcat
      901 gccagttcag aatcagaaga catgtatgct gctattgata ctttggtaga caaattaaat
      961 cgacaactga ttaaacataa agaaaaactt ttagcccata gagacaggga ttaaatgtta
     1021 ttttaaatac aaaccctttt actttaattt aatatcaaca ccttctcagt gcttatgtaa
     1081 actgacataa aacaaataac cgttttttag atgaagtcat agcactgaga aagagggttg
     1141 attactaaaa tcacttattt aatcactgta cctgatgcca gagtcatgat acattgtacg
     1201 tgctatgatc aagaccaaaa taaagataat caacagactc ggtttacatg ctcgtgcctc
     1261 agcgaaattt gtctcaacag cagcaaaatt tcaaagccat attgatgtaa ccaaagatac
     1321 tcagaccgtt aatggcaaaa gcattatggg tgtgatgatg cttgctgcta acaaaggtag
     1381 tgagttgacg ttggaaattg acggtccaga tgaagaacgc atgaatgaag ctttagtcga
     1441 attaatcaat aatcgatttg gggaaagtga ataaagaata ctaaactacg ctgtttacct
     1501 ctgctccata tctttttgcg acacacagag gttcctgttc tccatcaatg ttcctcgcag
     1561 ttcttgtaac atctttatta tgatcaacct gtgattcaat aaactgacct aaggtctcaa
     1621 tacaccttaa ttgatactaa tctgaagaaa tctactgttc acaggtctgg gtaacagatt
     1681 tacataataa ttcatcaaga aatacacaat ccatgtcatt tgctcatatt tcttctttgt
     1741 ttcctcattt tatgtaatga aatcaccgtc aacatagggc ctgacaaagc ataaatacct
     1801 gaacttgcaa aaagcactat tgcaggattt gcagcaattg caacaaacaa aagaatcatc
     1861 actaaaacat ataaaaatgg aaccttaccc ttataatcta cttctttaaa actgtaatag
     1921 cgtaaattac tcaccatcaa tacggaaaca atcaaagcca ggacagctgt aaataaagat
     1981 acaatcaaat ttgcccattc attttgaaaa cagatccaag caaaagaagc cattactgct
     2041 gcagccggtg ggcatgggag gccctggaaa taacgtttat ctgattttcc aatctgagta
     2101 ttaaaccgag cgagacgtaa cgcaacagcc gctgtataaa taaatgcgac caaccagcca
     2161 aattttccta attgatgcag agttaaatta tataacagca aggatggcgc tactccaaaa
     2221 gtaaccatat cagacaaact atcgtattga gcaccaaact ctgtttgtgt attggttaac
     2281 cgggcgatcc gtccatcaag actgtcagcg atcattccta taaacatagc gacaatagca
     2341 gcctcatatt gtccctttgt ggaagccact attgaataaa atgccgcaaa taaactgaca
     2401 gtcgttaata aatttggtaa caaataaatc ccagaatgat tttctttatt cattcgcaat
     2461 catacctcat tcatttttaa atcaaggaca tgctatcata agtagaacta aatctactat
     2521 ggaaacttat gcttagtcat aaagacagca tgcaattgtg actaataatt ggcaatgact
     2581 aaagcgaaac aaccatttat tgcaataaaa taattaattt tccctgcagg cccagcatga
     2641 tgatgatata attatcatac tgtatttaat atgcatagag ttatgtctaa tcgaaaaaat
     2701 aaaactattg tcattgaggg tgttacttca caaggcaaaa catttcgccc aagtgactgg
     2761 gccgagcgta tgagcggttc tttagctgtt tttaaaaata gtagaattta ttattctccg
     2821 ctcttgcaac caagcgttaa tagtgagggt tataaatgtg ttttacttga tcccaaactc
     2881 aaggaatcaa gcccacaagt ttatcaggca attatggatt ttgctaaagc taacaacttg
     2941 aaaatctgtg gtgaagaaga cttataacat tccaaaggat tgccctccag ttttattttc
     3001 tcaggactta ggaaaacccc cagtttcttg gttaaaaaca tttttgcctc gatcttgggg
     3061 tttttcctaa gtcctgtttc tttaaagcgc agacaaatca atcagaaaaa ctagaatccc
     3121 gctgcggctt ttactgataa agttgctttg acaaaataat ctaaagcaga attaatcatg
     3181 gcgatcgtat tatcaaaatt cattgcaaaa acgcctgcaa taagaaatac caatcctttt
     3241 aatccttgtt gagtacctgg ctggctggaa tgagcgacaa gtacacatcc acgtataaac
     3301 cacagaacac ccgccgtgcg aattaaaaac cccattgaac tgtagatatt ggcaggatta
     3361 taattggaat aagttaatac attaccgaca ccaaaagcgg tatttgccat aagatccata
     3421 cccgaaggca agtagattaa aagtgcccca actaataaat acatcattgg agtaaacatt
     3481 ttttcctgtg aggaggattg agcccgatga tccccgattt tttttaactt gccaattgcc
     3541 gttaaaaaaa ataagacacc caatatataa gctcctcctg tgataagttt ttgcacagga
     3601 taaagggatt ggctaatatt acctaatata gtgattaaat ctatcgtgtt catatgttaa
     3661 ttatatgcgc ttagctcttg caatgcataa tttttcccct atattaatca ttttagaata
     3721 aaagttgata ttccttatgc aaataaatac tttccccatc actctagaag agtctttcat
     3781 gatttctccc aaggtaaagc actttatatt caattgtgaa ttatcacccc cttttgaata
     3841 tttaccagga caatttatca ctattcattt tgaacatgat ggaaaaaact taaagcgtag
     3901 ttacagtatt gccaatgaac ccaaaaagga taatcgcatt gaatttgctg ccgggtattt
     3961 cgaaggagga ccaggcacag aactactcta caatttaaag ccaggtgatg tgatccacat
     4021 taatggacct ttcggtcgtt tgatttttaa agatgaaact ccaggaagat atatattggt
     4081 agcgactagt actggtacca caccttatag agccatgctt aatgagcttg gtcaaagaat
     4141 agaaaaacat cctgatttac aggtagtcat ccttcaagga gtgcaacgca gtgaagagat
     4201 tctctatcca gatgattttc aagcctttgc aaaaaaatat cctcaagcaa gttttcgtcc
     4261 ctatttaagc cgtgtgcaaa aacaagactt gaaagacaat gaatacagtg gttatgtaca
     4321 gcatgcattt ccagagctta atttaaatcc aactcgcgat atggtttatt tatgtggaaa
     4381 tccaggtatg atcgacgagg catttaatta tttaaaagaa catggttttt ccatgcaaca
     4441 tattgtacgt gaaaaatata tttcacgctg aaataccgca tcaaaaacca ttgttaatca
     4501 gtgtcatcct ttcggtaaat aacccggatt tgacaaaaga tgactaatcg ttatttaaat
     4561 ttaataggtg ccaaaccact tcgaaaggtc gacacccgat aatgaggaga taaacacgct
     4621 atgagttatg aagaattatc aaataccatg gagcaaatgc tacatgaagg aaaaggtatt
     4681 ttagctgcag atgaaagcaa cagtacaatt ggcaagcgct ttgcgtcaat ccatacagaa
     4741 aatactgagg aaaatcgaag agattatcgc ttgttactag cgaccactcc tgatttagaa
     4801 cagtatatca acggcgtcat tttatttgag gaaactttta ctcaaactga tgagcatgga
     4861 acaccgattc ctgagctttt tgctaataaa ggcattattc ctggaattaa agtagacaaa
     4921 gggcttgttg atttagccaa tacagaacat gaaaaagtca cccagggatt agatggttta
     4981 actgagcgtc tgcttcattt taaaaaatta ggtgcaaaat tcgcaaaatg gagaaacgtt
     5041 tattcaattt ccgaatacac tcccagccta actgcaatca aaacaggagc tgaaaatttg
     5101 ggccgttacg ctgctgcttg tcaatcagtc ggcatcgttc caattgttga gcctgaagta
     5161 ttaatggatg gcaaccatag catagaacat tgtgctgaag tctgtgaaat ggttctccat
     5221 gagctgtttc ataccttgtt tatccatcaa gtggagttgg agcatattat acttaaaccg
     5281 agtatggtta cctgtggtaa agaagtgacc ccgtttagcg atcctgaaga agtggctgac
     5341 tacaccatta gtgtatttcg taataatgtt ccagcgtcag tacctaccat taatttcctc
     5401 tccggcggcc aaacacccca acaggctacc gctaacttga atgcaatcaa tcaaattggc
     5461 caccaaccct ggttattaag tttttcattt ggaagagcat tacaagagga ttgcttaaag
     5521 gaatggtgtg gaaacaaaga aaatattgcc aaagcgcaag aggctttaat taagagagcc
     5581 cgcttaaaca gtatggcttg tttgggtgaa taccaaaacg acatggagta aagttgcaat
     5641 agcgacagaa ccttctctcg accaggaaga ctgtcgcttt aatcttaatc atacaaggct
     5701 tactaaaaac cccaaggtca aggcaaaaaa attttaaatg agggagttgg aataaaccaa
     5761 atgaccgcat taaaaatctt tttcaacaaa gagactggag gtagccctgt cataataatt
     5821 caaaacgtgc atacaagggt aaatgatcag ataacatccg ccaaggttta ccctgtaaac
     5881 aagcaacctc ttgaactttc atcccgcgaa aatacaccct gtcgacacac aaagcaggtt
     5941 ttattgcagg aaatgaacgg gcatgttgtc cttcaagcgc gacaaaggct tcttcaatcc
     6001 ctaagttttc agcaaggatt ttagaaataa catgacgcca atcattaaaa tctcctgcca
     6061 ttatcaaagg ctcattttta ggcacaacat ctttaatacg ttgtatcaat gccttacatt
     6121 gctcagtacg ctctgcctta aatagaccta aatgaacaca caaaagatga ataatattat
     6181 tatccaattt taattgggta tgaagtatac ctcttgatgc tctgttcatg tttgaaagat
     6241 taatactttc aaaactctcg aacgggtatt tgcttaaaat agcatttccg tggtggccag
     6301 attgatatac tgcattcttt gcataaacgt aatgtggcca tatattctca gcaatgtact
     6361 cacattgaag ggaatcaggc caactgacaa ttcttttttc acgttttcta tgtttacctt
     6421 gcacttcttg taaaaaaaca aagtccggat ttaatccagc gatagcatct cgcatttctg
     6481 gaagtaaaaa ccttactgca cctactccaa aacctttatg aatattatat gttatcagcg
     6541 atacgctgcg cacctcaggt tgcatagata ctctatctct catttaggtt tctataatca
     6601 gtataatggt aataggatta atgtattgct ctataattat aacccacatt ttgaaaagaa
     6661 tttgtcatta acatgattag ctcggactct gaattatttt tatttatcat aatattggcc
     6721 cttccccaaa aaccagtaaa gccatttatt ttgtgatctt ttatagtttg ttacaagtca
     6781 acccaataat cgacttactt tattgctttt tgtctatttc aattactatc aagacttgcg
     6841 aaaaaaacca agaccaaggc aaaaattgat tttaatgaga gagattaaat aaactaaatg
     6901 actggattaa aagcattttt tagcgaagag attgtttttt tcgcaagtcc tgactatttt
     6961 aaaaactgaa tttacatccc tgcatataaa gtaataagct ataactatta cctacaaacc
     7021 aaagaaaatt gatgaaaaca aagattttaa gtatcccggg actatctata gcatgcaaag
     7081 catggggaaa tcctgataat ccgcctattc ttgccttgca cggttggtta gataatgcta
     7141 atagttttga tgatattgct gaacatttac agaatgatta ctattttata gccgtagatc
     7201 tgcctggaca tggtcattct tcacacttac ccccaggatg catatatcat tttactgatg
     7261 gaatatttac tgttgttgaa attattaatg ccttgggact aaataagctt cacttgctag
     7321 ggcactctat gggagcctgc ttgggaagtc ttgtagcagg ggttgcaccg gatcgttttc
     7381 tttcattaag cttaatcgaa ggtttaggcc ctttttctca cccggcagaa acagcatgcc
     7441 agcaactgtc aaaatacctg gactatctat cacaaaaaca atcaaaaaag gcaaaaggct
     7501 acaataaatt tgaacatgct gctcttgccc gctccgttaa aggatatgtt tctctggata
     7561 ttgccaagtc actttgcgaa cgcggaattc aacaagaaaa tggtctatat tattggagac
     7621 acgatcgcag gctattagcc ccatctccat tacaaatgac tgaaacacaa gttctttcgt
     7681 gccttacaga aataaaagct caaacttacc ttatttgggc cagcaaagga ttttcatttg
     7741 attcggataa gatgaaagca aggattcaag cagtaaaaaa tattaaaatt gagaggttag
     7801 atggtggaca ccatattcat atggaaaagc cagaagtcat cagtcgactt ctggctaaat
     7861 tttatcaatc aataaaatga attaatcgac ataacaagat tgataattta tagtcacttc
     7921 actcaatgac ttcttaacat catccagatt gtaacccagg tctttggcag cactcaacat
     7981 accgcaacca ccctcatcaa atgttgaata aggtgtccaa taatccatat tggctttcac
     8041 catgacatca aaagccatac gaagattcca attaggttga ttggctaata tgtagaataa
     8101 atggttatac actccactgg agtaatgaac atctaacccg ccataataat catcagctac
     8161 atcaatagac ataccatcac ggcttggttt atccatgtaa cgcaatgcgt cataaccact
     8221 gtcttctttc attatttccg ggccgatttg ccagctgttt tttccaactg aataatattc
     8281 agcagcttgg gcagccatat ctgaaaacga ctcattcatg ccaccagact gaccgaaata
     8341 ttcaagtcca gaatgttgtt cggtgaaccc gtgactgatt tcatgaccac ctacacctag
     8401 agaaaccaaa ggatacatca tggtgtcacc atcaccaaag gtcatttgtt tgccgtccca
     8461 gtaggcgttt tcatagcctt gaccataatg tactctcata actaattgca ttggcgatcc
     8521 atctgattta gtcaatgcct ccaccccata ccaatcatgg tacatgtgtt taatgacata
     8581 acctgcatac aatgcgtcat tggtaggaga agcagctcca ttatctctat cataaccatc
     8641 agccgaatat ccggtataat aagttttagt agattgagta tcgggagtct ctttacaagt
     8701 aaattgcata ggtttgttat ttgaatagta cttatgtccc atgtcgacta ctttaacatc
     8761 ggtattttcc ataaagcaca tttcaacact tgaatcacgt gtgatttcta ataatggcaa
     8821 gtccttgcca aattgatatt caccaatttt acgatttcca ccaaagccca tcccttttgc
     8881 ttgaactttt tccgttttta catcatccca ttgaacaaaa ggcttattgg tttctgcatc
     8941 aataattgct gttggtcgct caggaattct atcgtcatgg ataactaaaa cgctgacttt
     9001 gtaagcccaa tgagcttgat gtttttcatc aatataaatc attggggtta cttgatcttc
     9061 actaatttgt ttattcgcgt atttatcctt aaattgttgt aatgctaaag aggcattttt
     9121 cacaaatgaa ggtttaggct ggcctaattc tgtttgcaac ccatcataaa tcacaccatt
     9181 cattttcaca tcagattttg ccgtcgctaa actaggagtg gcattcttac tgtgcaggat
     9241 agcgtaacca ccaaaaactg gaaacccggc atattgttgc tgcatacgaa catgcgtcac
     9301 tttattccca tcggtatgct gtcttataaa ctgcaaacta tttgttgaaa ctacagcacc
     9361 tttcataaca ccaggcaaag tcaactgaaa tttttgtgtg acttcagaaa aagacgactt
     9421 ttgtaaaggg atcggatcgg ctgccttcac aggtgaagca atccctaatg ctatagctac
     9481 agctaaaggt gacaaataat aatttgggtg cattgattct aactccttaa actaaattct
     9541 catacagcaa ctcttcctgg agtgcccagg tttgttatat tcccctataa tttttcagtt
     9601 aattttttag cgaagactaa tgctcaactg caaaattaag gatgatccaa atcaggacaa
     9661 tacttaagat aataaaaaag tattttcctt ctttaattca actatttgga tagtatccta
     9721 atatttctag gcatataacc aactaccatt ttcaaaatgc aagaactctt ttccaaactt
     9781 ttccaaacta gcacaaattt attatttttg aaaatattaa ttttgctcag atgcaaaact
     9841 tgagtttatc cgacattttg aaaagccaca gactgatgga cttgggctct ctaaataatt
     9901 tccaaaaaaa attgacctaa gtggtgcaat tttaacctaa ttaaa
//
LOCUS       DASLOE010000113         9939 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.114, whole genome shotgun sequence.
ACCESSION   DASLOE010000113 DASLOE010000000
VERSION     DASLOE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9939
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.114"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1040
                     /locus_tag="U6309_002121"
     CDS             <1..1040
                     /locus_tag="U6309_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EbhA"
                     /protein_id="HEN5505559.1"
                     /translation="QPATDLPEFITREDIFKLLSFKIFMPLAPIFAEREKWMKRIAGT
                     DGKNAHASENYNKFKKIFLNSHHKFVIEESLSHFEHGTLKYHHEWLTAKRQIAKEVKS
                     LRALQENWKEKKNKIDRKKASILFDFLDSFESIPNQLRSREEEGFKQTEVYKKALSTD
                     KLKAIELLNQHLFDYSNPQEELAERMKLIKALYENNILFEQHHTPIGWLKNQLNIETV
                     LDKKLASLEQSLKAAENALNSYMEHAMPKTVTISTELRKESEKDSIPLPEKNKSPEAV
                     SENQNTSTSNPEIQPQTNPVQNRKNTGKVYLKVQQYEENLRRLGVFTPKTPHCSESLK
                     KDPADGNRISL"
     gene            1233..2813
                     /locus_tag="U6309_002122"
     CDS             1233..2813
                     /locus_tag="U6309_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEN5505560.1"
                     /translation="MFDLAKLTEFNHALVYGFEPESSPARLAGSETFEDALFPEAGQH
                     QDMLNAYLYAQETILPQIKKTGLSHITPEMLIEWVKAIHGFIGKSLMQAYDRKSGEYT
                     NEIIFRWHLGAELGTHFTLYLSGLHECKSPQQLARYLNKQFDMDYQSALDFIALLEKI
                     AKDKKYTIHESLQPYIDYSSPAIKGILVQNKLASAYNLNQLTESEKITVNKILKICMF
                     PPLIPEAMNRWARETTVQLRACDSRDLKNVSEFLATTFYQLTEVHPFANANGRTATCL
                     INTFLRAFGYPSIVLRHPGERENKDSRYQKAFAEIDSSLIPLIDLIYTRVNEAQEKAF
                     ENEKLKKLIVLRVALSDLLQETKSKHPQFNLIAFQKQVFSSPETLFAMQMADETEASI
                     FVLSMSINKLSQIAKELDLSKQKKPTLFSTAVLDSTQINAVIKSLEKISGLPGWKTNA
                     KKGLVTWLELPDMEKAKEIASELEALKVAKITVARRADNKIPVIKCEEIDCEKLVNVA
                     LQKDGKTSSKNSEFVATF"
     gene            3080..5074
                     /locus_tag="U6309_002123"
     CDS             3080..5074
                     /locus_tag="U6309_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1959 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505561.1"
                     /translation="MLISTRILEPALSHFNDRNNLIKQLDLSSDGDNNSFYKWVLAFW
                     LGSNITREKIIKHPLYIKCEAFIDLGYSCILVLDKQMSLLKQNSLAYKILQKNLFEII
                     QHYNDNYPLLTQNPQTLFSFFANILAKIDSKVCEDISHRKILELTQNTCLAELSRTQN
                     GPPDGLAATQVSNDVTSLMKVNPFNIGIAINKLITENFSKELFFPHYIKPTTDKHITH
                     KLLIPAWDGIVLEGLSVKGRKKTNNTVVLALIGHFQTEHHYLNTSFHEFQELFGTELV
                     LINHRNYSNRSNKFANSAEEIARDVIAFVNHFRQKNKKIVLYGMCGGAAHMILAAHML
                     SHQKIPFKLIVDRFSQKYINFVDFKTLSRARDFSRSNGQDCSRLLPGYKYYPGLMPYL
                     LILLFLLLFILVQLGLFLTKTNIDFAKLVRMIPEEDLLILQAKGEKIATSKKPFFTDI
                     IVHPENDMRAAVKDKRKQRKTILKNLCEHCLHAAGQAVFSAEMQKIFLQLFNCFDQCL
                     QLINNEKLMENTITNRPVDLHSKKLYTLTTRNKLPISQFIRGFFKQSPKMHAHLLDSI
                     KPYSSHLIVDALKQIYGNHPSIHSNLLQFSNHLALLLNDMKTNQIFISYMADRLSATQ
                     LADLNEPINVLLRSELLQLIFKSSSEQNNQDIINNHRVSI"
     gene            5259..6032
                     /locus_tag="U6309_002124"
     CDS             5259..6032
                     /locus_tag="U6309_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505562.1"
                     /translation="MFFSKNEKNPIKKALQGELLQDEPFIQLCTRIENFLMDTEAVNE
                     QLIELNEQLTMRLKEKGLKPGEKGATKQLRSLIQEILTEAGFREGMLQTIGNKPLKKE
                     DFMFLVSSGFMLKDSSLRGSSHGELTHAIQWCLIILKQKKDSNFLENIPVNKICDQIY
                     KKLGHKDSSNPSHPFTCWDMLIDQPGVEDARSPEWLSEHIQNDGEQIFPVLKEVIKNR
                     TKKGQTEENKEKLQKKLENPPEHYEKHEEIENILMPKPK"
     gene            complement(6117..7151)
                     /locus_tag="U6309_002125"
     CDS             complement(6117..7151)
                     /locus_tag="U6309_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEN5505563.1"
                     /translation="MIDNSVKIIKHKVGLLNLAEELGNVSKACKVMGLSRDTFYRYKS
                     AVESGGVDALFDQTRRKPNHKNRVDESIELAVKEYAIEYPAHGQQRTSNELRKKGIFV
                     SGSGVRSVWLRHDLANFKDRLKALEAKVASEGIVLTEAQVIALERKKFDDEACGEIET
                     AHPGYLGSQDTFYVGTLKGVGRIYQQTFVDTYSKVAFAKLYTTKTPITSADILNDKVL
                     PFFEHHQLPMLRILTDRGTEYCGKVEQHDYQLYLAINNIDHTKTKAQSPQTNGICERF
                     HKTILQEFYQVTFRKKLYDNMDELQKDLDEWLHYYNNERTHQGKMCCGRTPIQTLMDS
                     KQIWMEKFIN"
     gene            complement(7270..8799)
                     /locus_tag="U6309_002126"
     CDS             complement(7270..8799)
                     /locus_tag="U6309_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN5505564.1"
                     /translation="MNDSENESDSEYDSDSESEYELRTLPSKIHFVWVGGPIRAQYLR
                     TIMDVAAVALKSGFEVNLWVDNEMNYIKTSTQEGIDIPNLRIRNIHELEEGMRNDPFY
                     EGDRYKKFWEYVNRERVGFRNLAAAADFFRFEILRQEGGYYFDTDTVFYLDENSQFVA
                     DEVAFGIKAHVDLYTGSLRDPHGRGPTIYHVNTLGDVNNDIIAAIPDHEVMEDAILNA
                     MQRHEEYDLSPLPRESLKKRFGPYGNLMDAKRTPYQLEGSMDFRKHQTIAAGPGALQD
                     ALRDYMEGVEVEDYNLLMTLKVGNESSRVANDKDKKPIMGIEVVSKCDKTWLTKINKA
                     KKRKARQKAFDTDSLAIEPALSKDSGQTASNREAYSDGESEYDGELTEEIEFQGNLRK
                     EQFINKLKDYIITKKEEARQALEVKENEEGHDSEEKILSKHFKTRHIELTKGFSVEQK
                     IAAVEKLISLLQEKDGAELLNAIDYAALTSSRLGKLVQRYDDLILKEYVVPDEYGEKP
                     Y"
     gene            8945..9511
                     /gene="lirB"
                     /locus_tag="U6309_002127"
     CDS             8945..9511
                     /gene="lirB"
                     /locus_tag="U6309_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system effector LirB"
                     /protein_id="HEN5505565.1"
                     /translation="MNKIICLLSNLLLTIGIMSAHAETAIIKTSEGNITCELFTKEAP
                     NTVANFVGLATGTKEFKDIKTGKMVKRPFYNGLTFHRVIAGFMIQSGDPLGNGTGGPG
                     YTFDNENTNASFNKPGVLAMANAGPNTNGSQFFITVAPTPELQGNYNVFGQVISGQEV
                     VDKISKMPTDPQDKPITPVVIENITIQR"
     gene            complement(9592..9939)
                     /locus_tag="U6309_002128"
     CDS             complement(9592..9939)
                     /locus_tag="U6309_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505566.1"
                     /translation="MNGKSGSEELNIIFNYENIKNKNPHDLFNELFKKFTEDVGYGMG
                     SRSIEALKRALAKNDITYTDGYYGKTATLTSLLWSKEPPVITDEKNIFKLLDKKHLSE
                     NKEDVLGKGIRYI"
BASE COUNT         3316 a         1937 c         1671 g         3015 t
ORIGIN      
        1 agcaacctgc aactgatttg ccggaattca tcacccggga agatattttc aaacttttaa
       61 gctttaaaat atttatgcca ttagctccga tattcgcgga gagagaaaaa tggatgaagc
      121 ggattgccgg tactgatgga aaaaatgctc atgccagtga aaactacaat aaattcaaaa
      181 aaatcttttt aaactctcat cataagttcg ttattgagga aagcttatcc cattttgaac
      241 acggaacatt aaaatatcac catgagtggc taactgcgaa gcggcaaatt gccaaggaag
      301 tcaagtcatt acgtgcatta caagaaaact ggaaagagaa aaaaaacaaa attgacagaa
      361 aaaaagcttc tatcctgttt gattttcttg atagttttga aagtattccc aaccaattac
      421 gctcaaggga agaggaggga ttcaaacaaa ctgaagtcta caaaaaagca ctttccacag
      481 acaagctcaa agcaatagaa ctcctgaatc agcatctttt tgattacagc aatcctcaag
      541 aagaattggc agaaagaatg aagcttatca aagctctgta tgaaaataat attctatttg
      601 aacagcacca tactccaata ggatggctga aaaaccaatt gaacattgaa acggtacttg
      661 ataaaaagct ggcgtctctg gagcaatcgt taaaagcagc ggaaaatgcg ctaaattcat
      721 atatggaaca tgcaatgcct aaaacggtca cgatttccac tgaactaaga aaagagtcgg
      781 aaaaggatag catccctctc cctgagaaaa ataaatcacc tgaagcagtt tcagaaaatc
      841 agaatacaag tacttctaat ccggaaattc aacctcaaac caatcctgta caaaatagaa
      901 aaaacaccgg caaagtttat ctgaaagtac agcagtatga agaaaacctc cggagacttg
      961 gtgtatttac tcctaaaacc cctcattgct ccgagtcatt gaaaaaagat cctgctgatg
     1021 gaaatagaat ctctctataa aaacaagcga aacgcctttt atagtctttg tgaaaggact
     1081 cacaaatagc tattgagata gacctaaaaa atgcaaaagc ttctataggc gaatacttaa
     1141 acctcgacaa aaggacaatt aaagagcaca gtggatattt attgcgttct ctgctacgat
     1201 cattcccctt ttaattaatt acaaaaacaa ccatgtttga tttagcaaaa ctgactgaat
     1261 ttaatcatgc tttagtgtat ggatttgaac cagaatcaag ccccgcacgt ttggcaggct
     1321 cagaaacatt tgaagacgct ctttttccgg aggcaggcca gcatcaagat atgttaaatg
     1381 cctatcttta tgcacaagaa acaattcttc cgcaaattaa aaaaacaggt ttatctcata
     1441 ttaccccaga gatgttaatt gagtgggtta aagccataca tggctttatc ggaaaatctc
     1501 taatgcaggc ttacgataga aaatcaggtg aatatactaa tgaaattatt ttccgatggc
     1561 acctgggtgc cgaactgggg actcatttta ctttgtattt gtcaggtctc catgagtgca
     1621 aatccccaca acaacttgca agatatttaa ataaacaatt tgatatggat tatcagtctg
     1681 ctttggattt tattgctctg ttagagaaaa tcgccaaaga taaaaaatat acaattcacg
     1741 aatcactgca accctatatc gattattcca gtcctgcgat aaagggaatt cttgttcaaa
     1801 ataaattggc gagtgcatat aacctcaacc aattgacaga gagtgagaaa attacagtca
     1861 ataagatttt aaaaatttgt atgttccctc ctctgattcc tgaagccatg aatcgatggg
     1921 cgcgagaaac aacggtacaa ttacgcgctt gtgattcaag ggatttgaaa aatgtttctg
     1981 agttcctggc aaccactttt tatcagctta cagaagtgca tccttttgcc aatgctaatg
     2041 gaagaacagc aacctgtctt attaatactt tccttcgcgc atttggctat cctagcattg
     2101 tgcttcgtca ccctggggaa agagaaaata aagacagtcg atatcaaaaa gcattcgctg
     2161 aaattgattc ctctctaatt ccgttaattg atctgattta tactcgtgtc aacgaagctc
     2221 aagaaaaagc ttttgaaaat gaaaaactaa aaaaactgat cgtattgcga gtcgctttaa
     2281 gtgacttgtt acaagaaacc aagtctaaac atcctcagtt taatctaatt gcattccaga
     2341 aacaggtgtt ttcttctccc gagacgttgt ttgcaatgca aatggctgat gagactgaag
     2401 catccatttt tgtcctgtca atgagtatca ataaattatc tcaaatagca aaagaattag
     2461 acctgtcaaa acaaaagaaa ccaacattgt tttcaacagc cgtgttggat agcacacaaa
     2521 taaatgccgt gataaaaagc ctggaaaaaa tatctggtct gcctggctgg aaaaccaacg
     2581 caaaaaaagg attggtcact tggctggaac ttcccgatat ggaaaaagcc aaagaaatcg
     2641 cctctgaatt ggaagcgctc aaagtagcca aaatcaccgt ggcacgacga gctgacaata
     2701 aaattccagt gattaaatgc gaagaaattg actgcgaaaa gcttgttaat gttgctctcc
     2761 agaaagatgg taaaacatca tcaaagaact cagaatttgt tgcgacgttt tgataaaaaa
     2821 tgagcctgta tttaaagagg caggccaaaa acaacaacaa attacagtga gggaaaaaac
     2881 agcaggataa ataatttgtt tcaatttaag cttgatctgg ccaatgccag ttttttataa
     2941 aactgcggcc ggatcttatt gagccaaaac gacaggaaat actgagcaca attttttcat
     3001 agcaacccaa agatgacatc tatccatttt gatcgagata gaacttaaat gaattttctt
     3061 ttattacgtt tttgaactta tgttaatttc taccagaatt ctcgagccag cattaagtca
     3121 tttcaacgac aggaataatt taatcaaaca attagattta tcatctgatg gcgataacaa
     3181 ttccttttac aaatgggttt tggcgttttg gttgggttca aacatcactc gcgaaaaaat
     3241 tatcaaacac cctttgtaca taaaatgtga agcttttatt gatttgggtt actcgtgcat
     3301 tttggttttg gataaacaaa tgagcttgtt aaagcaaaat tcacttgctt ataagatcct
     3361 gcaaaaaaac ttatttgaaa taattcaaca ttataatgac aactatccgt tattaaccca
     3421 gaatccccaa acgctatttt ctttctttgc caatattctt gcaaaaattg acagcaaggt
     3481 atgcgaagat atttctcacc gaaaaatcct tgaactgact cagaatacgt gcctagctga
     3541 attatcgcgc actcaaaatg gcccgccaga tggattggca gcaacacaag tgtctaatga
     3601 tgtcaccagt ttaatgaaag ttaatccgtt caatataggt atcgctatca ataagctaat
     3661 tacggaaaat tttagcaagg aacttttttt cccacactac ataaagccaa caaccgacaa
     3721 gcatattact cataaattat taataccagc ctgggatggt attgtacttg aggggctttc
     3781 cgttaaggga agaaaaaaaa caaacaatac cgttgtttta gcattaattg gacatttcca
     3841 aacagaacat cattatctta ataccagctt tcatgagttc caggaacttt ttggcacaga
     3901 attagtgctt ataaatcata gaaattattc caatcggtcc aataaatttg ccaacagtgc
     3961 tgaagaaata gccagggatg tgatcgcttt cgtaaaccat ttcagacaaa aaaacaaaaa
     4021 aattgtactg tatggaatgt gtggcggggc tgcgcatatg attttagcag cccatatgct
     4081 atcccatcag aaaatcccat ttaagttaat cgttgacaga ttctctcaaa aatacatcaa
     4141 tttcgtagac tttaaaacac tctcgagagc cagagatttt tcacgctcaa acggacaaga
     4201 ttgctcgaga ttgcttccag gttacaaata ttatcccggt ttaatgccat atttgctcat
     4261 tctccttttt ttattgttat ttattttagt tcaattgggt ctatttttaa caaaaacaaa
     4321 tattgatttc gcaaagcttg tgcggatgat tcctgaagaa gaccttctga ttttacaagc
     4381 caagggagaa aaaatagcaa cctcaaaaaa accattcttt acagacatca ttgtacaccc
     4441 agaaaatgac atgcgggcag ccgtaaaaga taaaagaaaa cagagaaaaa ccatcttgaa
     4501 aaatttatgt gagcattgtt tgcatgctgc tggtcaagct gttttttcag ccgagatgca
     4561 aaagattttt cttcaattat ttaactgctt tgatcaatgc ctccagctaa taaacaacga
     4621 gaaattgatg gaaaatacca tcactaatcg ccctgtagat ttacacagta aaaaacttta
     4681 cacccttaca accagaaata agttacctat cagtcagttc attagaggtt tttttaaaca
     4741 atcaccaaaa atgcatgccc atttgcttga ttcaatcaaa ccatactcta gtcaccttat
     4801 agttgatgct ttaaaacaaa tttatggaaa ccacccatcg atacattcta atttattgca
     4861 attttctaat catttggctt tgcttttaaa tgatatgaaa accaatcaga tttttatttc
     4921 ttatatggcc gatcgtttat ctgctactca attagctgat ttaaatgaac ctataaatgt
     4981 gctgttaaga agcgaactac ttcaactaat tttcaagagt tcgtctgaac aaaataatca
     5041 agatatcata aataatcata gggtatccat ttaatgcatt cattgccccc actaattggg
     5101 ctatcgttga tactaaatgg caagattaat gcaaatgcgc ttaaacaatg tgctcatcaa
     5161 cactcaatca aaccaataag taagcaataa taaatattta taaacaatat ttattaaaaa
     5221 taatctatac ttaaaaaaat tagcgcgttg gaggtaatat gtttttctct aaaaatgaaa
     5281 aaaatccaat aaagaaagca cttcaaggtg aattattgca agatgagcca tttatacaac
     5341 tatgtacaag aatagaaaac ttcttgatgg ataccgaagc tgtaaatgag caactcattg
     5401 agttaaatga acaactgaca atgaggttaa aggaaaaagg actcaaaccc ggagaaaaag
     5461 gagcaacaaa gcagttaaga tcgttaatcc aggaaatact gacagaggca ggctttagag
     5521 agggaatgct gcaaacaata ggtaacaaac cattgaaaaa agaagatttt atgtttttgg
     5581 tatcatccgg atttatgtta aaggacagca gcctgagagg gtcatctcat ggagaactga
     5641 cccatgcaat tcaatggtgt ttaatcatat tgaaacaaaa gaaagacagt aattttcttg
     5701 aaaacatacc tgttaataag atttgtgacc aaatttataa aaaattaggc cacaaagatt
     5761 cttcgaatcc gagccatcca tttacttgtt gggatatgct tattgatcaa ccaggagtag
     5821 aagacgccag atcaccagaa tggctttccg aacatattca aaatgacggg gagcaaattt
     5881 ttcctgtatt aaaagaagtg ataaaaaata gaaccaagaa gggtcaaaca gaagaaaaca
     5941 aggaaaaatt acaaaaaaaa ctggaaaatc cgccagaaca ttatgaaaaa catgaggaaa
     6001 ttgaaaatat tctcatgcca aagccaaaat aaatttgaac tactacaact aatttgtagt
     6061 agttcaaact tgatctgaca gttgccggtt tttcagaagt gtctgtcagg tcaaattcag
     6121 tttataaatt tttccatcca gatttgtttg ctatccatca aagtttgtat cggagtgcgt
     6181 ccacaacaca ttttgccttg atgggttcgc tcattattat agtaatgaag ccattcgtcc
     6241 agatcttttt gaagctcatc catgttatcg taaagtttct tacggaatgt aacttgataa
     6301 aactcttgta aaattgtttt atggaaccgc tcgcagatac cattggtttg tggcgattgc
     6361 gctttggtct tcgtatgatc gatattgttg atcgctaaat aaagctggta atcatgttgc
     6421 tcgacctttc cgcaatactc tgtgccccta tcagttaaaa tgcgcaacat cggtaattga
     6481 tggtgctcaa agaaaggtag aactttatca tttagaatat cagctgatgt gattggagtt
     6541 ttcgttgtat atagcttagc aaatgctact ttgctataag tatcaacgaa tgtttgctga
     6601 tagatcctgc ctacaccctt gagtgttccg acataaaatg tgtcttgaga acccaagtaa
     6661 ccagggtgag ctgtttcaat ttcaccgcag gcttcatcat caaatttctt ccgttccaaa
     6721 gcaataacct gggcttcggt taatacaatg ccctctgatg ccaccttggc ttcaagtgct
     6781 tttaatcggt ctttgaaatt ggccaaatca tgccttaacc aaacgctacg tactccactg
     6841 ccagaaacaa agataccttt ttttcgtagt tcattacttg tgcgctgttg gccatgagca
     6901 gggtattcta tagcatattc ctttactgct agttcaatag actcatcaac tcgattcttg
     6961 tgattaggtt ttctgcgagt ttgatcgaat aatgcatcaa ctccaccaga ctctactgct
     7021 gatttgtaac gataaaaagt atcgcgggat aatcccatta ctttacaggc tttcgataca
     7081 ttccctaatt cttcagctaa attaagcaat ccaactttat gtttaattat tttaacgcta
     7141 ttatctatca tgagagtttt cctcttggtt tgattaaaag ttcgcacttc tatcaaaacc
     7201 ggaaactctc accttttcaa gtggttatgt cagattaagt caaaactaat tcaactaatc
     7261 catttgaatt cagtacggtt tttccccata ctcatcaggc acaacatatt ctttaagtat
     7321 caaatcatcg tatctttgga ctaacttacc caggcgagaa ctggtaagtg cagcgtaatc
     7381 aattgcatta agcagttccg ccccatcctt ttcctgcagt aaactaataa gcttctctac
     7441 ggcagcaatt ttctgttcaa cagagaaccc ttttgttaac tctatatgtc gtgttttgaa
     7501 atgcttactt agaatctttt cctcggaatc atggccttct tcattctctt ttacttccaa
     7561 agcctgcctt gcttcttctt tttttgtgat aatgtaatct ttcaatttat taataaattg
     7621 ttctttgcgt aagttgccct ggaattcgat ttcttctgtt aattcaccat cgtactcact
     7681 ttctccatca gagtaagcct ctctattact tgctgtctgc ccagagtcct ttgaaagtgc
     7741 tggctctata gctagcgaat cggtatcaaa ggctttttgc ctggcttttc tttttttagc
     7801 tttatttatt ttagtgagcc atgttttatc acacttactg accacttcga ttcccatgat
     7861 cggtttttta tccttatcat ttgctactct tgagctttca ttaccaacct ttaaggtcat
     7921 taataagttg taatcttcca cttcaacacc ttccatgtaa tcacgcaatg catcctgtaa
     7981 ggcacccgga ccagctgcaa tagtttgatg ttttctaaaa tccattgatc cctccaattg
     8041 ataaggtgtg cgtttagcat ccatcagatt gccatatgga ccgaatcttt ttttaagaga
     8101 ttcccggggc aaaggcgata agtcatactc ttcatggcgt tgcatagcat ttaagattgc
     8161 atcttccatt acctcatgat caggaatagc agcaataata tcgttattga cgtcccctaa
     8221 agtattgaca tgatagatag taggccctct tccatgtgga tccctcaatg atcctgtata
     8281 caaatctacg tgggctttta taccaaaagc aacctcatcc gctacaaatt gcgagttttc
     8341 atccaaataa aaaacggtat cggtatcaaa gtaataccca ccctcttgcc ttaaaatttc
     8401 aaaacgaaaa aaatctgcag ctgcagcaag atttctgaaa ccgactcttt ctctattgac
     8461 atattcccaa aattttttgt acctgtcccc ttcgtaaaaa ggatcatttc tcatcccttc
     8521 ttctaattca tgaatattcc ttattcttaa gttaggtatg tctattcctt cttgagttga
     8581 tgttttaatg taattcattt cattatcaac ccataaatta acctcaaaac cactttttag
     8641 tgctactgca gcaacatcca taatagtgcg taaatattga gctcgaatag gtcccccaac
     8701 ccaaacaaaa tgtattttgg atggtagcgt acggagctca tattcagatt cagaatcgct
     8761 atcatattca gaatcacttt cattttcaga atcgttcatg aaactctcgc ataataactc
     8821 aatattgttt aattatagta cacccatatc taattcagcc gaaactccga gtttcattta
     8881 aaaaccattt taatacgaca taatactgtt atactgatct tagccattct atgaggaact
     8941 tattatgaat aaaattattt gtttactcag caacttattg ttaacaatag gcatcatgtc
     9001 ggcgcatgca gaaactgcaa ttattaaaac ttcagagggc aacataacgt gtgaattatt
     9061 tacaaaagaa gctcccaata ccgttgcaaa ttttgtaggg ctggcaactg gaacaaaaga
     9121 atttaaagac ataaaaactg gcaaaatggt taaacgccct ttttacaatg gcttaacctt
     9181 tcatcgtgtg attgctggtt ttatgattca aagcggagac ccgcttggaa atgggaccgg
     9241 tgggccaggt tacacatttg ataatgaaaa tactaatgcc agttttaaca aacctggcgt
     9301 acttgcaatg gctaatgcag gccccaatac gaatggcagc caatttttta ttaccgttgc
     9361 gccaacaccc gaattacaag gcaactacaa tgtttttggt caagtaattt ctggacaaga
     9421 agttgttgac aagattagca agatgcctac tgatccgcaa gataagccaa taacgcctgt
     9481 agtcattgaa aacattacca ttcaaagata aaagatcagt gcctgtgtca gattaggtgt
     9541 aaaccaattc attaaatata tattaaaact cggaacatgg gtacgtaaga actagatata
     9601 cctaatccct tttccgagta catcttcctt attctcactt aaatgcttct tatctaataa
     9661 tttaaatata tttttttcat cagtaataac aggtggctct ttactccata atagacttgt
     9721 taaagttgct gtttttccat aatatccatc ggtataagtg atatcatttt tagctaatgc
     9781 ccttttaaga gcctcgattg aacgtgaacc catgccatat cctacatctt cagtaaattt
     9841 tttaaagagt tcattaaata aatcatgtgg gtttttattc ttgatgtttt catagttgaa
     9901 tatgatattg agttcttctg aaccactttt accattcat
//
LOCUS       DASLOE010000114         9853 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.115, whole genome shotgun sequence.
ACCESSION   DASLOE010000114 DASLOE010000000
VERSION     DASLOE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.115"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..355
                     /locus_tag="U6309_002129"
     CDS             <1..355
                     /locus_tag="U6309_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lpg0716 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505567.1"
                     /translation="ISSIILISSLLRVLIDIEKDRQLIHQLKLNETSREQIKNEQLAM
                     TNARMKDLNKIILNSVFLPIGFFLLITTPIPLTIIACVSMLLVHWLITNLINMRYSPN
                     ISVSPTIQTAELSLC"
     gene            complement(456..917)
                     /locus_tag="U6309_002130"
     CDS             complement(456..917)
                     /locus_tag="U6309_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505568.1"
                     /translation="MKKILYVIPGILLITNSFALDIPGNITNSNKDLLPSPFPVYVIE
                     GSAVVNHPYPGAKKVLLPTDNGYVDYPGCYIACYSHNTGVYAISPTISVMGQIRVKGQ
                     YDARICQPDGYKNQDISAAYQFKQLCAEKIPACQNNSCWAGGDTGGWFGIQ"
     gene            complement(1006..2253)
                     /locus_tag="U6309_002131"
     CDS             complement(1006..2253)
                     /locus_tag="U6309_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEN5505569.1"
                     /translation="MVTQFNTLKSYIFPIILLLSILLGGLTGYVFNQNVAWLKPFGDI
                     FLNLIFTTIVPLIFFSVSSAIARTGSIGKLGKIFYSMTVIFLFTGIVAAVYALFVVLL
                     FPPAQNMPVLLESSKQLSPTNLPDRLIEIFTVPEFSKLFSHEHILALVLFSILVGLAV
                     AHSKDKGTIFNSFLKAGEDVFMSVFRLIMYYAPIGFFAYFAVIVSEIGPQLMENYLRI
                     AILYYTASVFYFFFALTAYAYLAGKSHGVRLFWGNIFLPAATSIATCSSAASIPANLM
                     ATKSMRIPAEIYETSIPLGSIIHKDGSVMGGVFKIAFLFGIFHLPFASLPVLMMALGV
                     SLLVGTVMGAIPSGGMLGELLILSVYGFPPSVLIAVAAISIIIDPIATMVNVTSNSVC
                     SMMIARLVEGKNWFLRSSDENNL"
     gene            complement(2375..5140)
                     /locus_tag="U6309_002132"
     CDS             complement(2375..5140)
                     /locus_tag="U6309_002132"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEN5505570.1"
                     /translation="MDKTYSPEAIEKALYKKWESHHYFQPRGEGKRFCIMLPPPNVTG
                     SLHMGHGFQHTIMDALTRYHRMLGDKTLWQPGTDHAGISTQLVVERQLEAQGVSRKDL
                     TREQFLDKVWQWKEESGNTITQQMRRLGASVDWSRERFTMDEGLSAAVQKVFVQLYEE
                     GLIYRGTRLVNWDPKLGTAVSDLEVLSEEEDGFLWHIRYPVVDSEEFLIVATTRPETL
                     LGDCAVAVHPDDSRFRHLIGKQVHLPLCDRTIPVIADDYVDKEFGSGCVKITPAHDFN
                     DHEVGKRHQLPQINILTKKGTINKNAPLKYQGMDRFVAREQIIKDLEQEGLLAKTEPH
                     KLKVPRGEKSNVIIEPLLTDQWYVKTKPLAEPAIAAVKKGDIRFIPETWDKTYFQWMD
                     NIEDWCISRQLWWGHRIPAWYDNHGNIYVGYSENDVRFKHKIDQSTPLKQDEDVLDTW
                     FSSALWPFSTLGWPERTPELEQFYPTSVLVTGFDIIFFWVARMIMMGLKFTGKIPFKE
                     VFITGLIRDSEGHKMSKSKGNVLDPLDIVDGIDLDSLIAKRTSNLMLNSVRDRITKAT
                     RKEFPEGISAYGTDALRFTYCSLASTGRNVRFDLGRVEGYRNFCNKLWNAARYVLLNT
                     DEEQIDFGDGAFQYSPADQWILSRLQNTVSKVHHYFETYRFDLLANTLYEFVWHEYCD
                     WYLELSKPILQDEQALSAMKRGTRRTLIHVLDQILKLLHPLMPFITEEIWQKTTKFTS
                     ENGISIMLSTYPKVNEEFINPAIEEELDWLKSAIQSLRTIRSEMSISPAKLIPLYIRN
                     ITPELKERIAKYEKILKTLSKIDKINYLAPDEKVPVSATAVLGEIELLIPMADLIDKE
                     AELSRLNKELAKLNKDIELAQGKLNNPKFTDKAPEEIIAKEKDKLAQAQLAKDKLLQH
                     KNRIESL"
     gene            complement(5397..8444)
                     /locus_tag="U6309_002133"
     CDS             complement(5397..8444)
                     /locus_tag="U6309_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEN5505571.1"
                     /translation="MKFTDLFIKRPVLSIVVSMLIFLFGINSIYKMQIRQYPKMENTV
                     ITIMTGYPGADAELIAGFITTPLESAVASAEGIDYMTSSSQQGLSTITLNIKLNFDPQ
                     IAFTDVMSKVQQTLNQLPKESQQPVILKKSDSSTALMYISLDSKDMTPQQITDYATRV
                     VQPQLQTVDGVAKADILGGQTYSMRIFMDPIKMAALNVTPADVSSVLASNNFLTAAGS
                     TKSEYVAINVTAKTDLNNVGEFERLIVKSDKGSIIRLRDIAKVELGSQNYDSSVTFNS
                     KKAVFISITPTPTANPLTVINDARKLFPSIVREFPPSLTGTIVYDATAFIRASIHEVM
                     ETIIEAAVIVIIVIFLFLGSVRSVLIPVVTIPLSLVGVCTLMLILGYSINLLTLLAFV
                     LAIGLVVDDAIVVVENVHRHLEEGKTPFESAIIGAREIATPVIAMTITLAAVYAPIGF
                     MSGLTGALFKEFAFTLASAVIISGVIALTLSPMMCSKILSEEATSGKFAIFLDEFFNK
                     LKRVYQNALHSLLDTREMMLLLAGVVILMLPYLYSNTAAETAPDEDQGFFFVMATAPQ
                     YATLDYIEAYTKPFDAIYKSFPETENYFTVNMSQPISGMVLKPWDQRKKSQFALKQPL
                     QNKLSKIAGLNAFAIVPPPLPGGGGGPAVQFVVKTTNDFQSLMDVSNKLTEKARNSGL
                     FIYIDNSLKFNQPQVEFSINRAKASEMGLDMRALGSSLTSALSGNYINYFNLEGRSYQ
                     VIPQLARKFRLTIEQLGQIYVKTLNGTMVPLSTVITPTEKTQPNAATHFQQLNSATIQ
                     AVMMPGKTLGQGLEFLQKAAQEVLPKGFTYDYGGESRQFMQEGSALIFAFLFAIVVIF
                     LVLAAQYESFRDPLIVLISVPMSICGALIPLNLGVATINIYTQVGLITLIGLISKHGI
                     LIVDFANHLQREKNLDKRAAVEEAAGIRLRPILMTTAAMVFGVIPLLVASGAGAASRF
                     DIGLVISTGLLIGTCFTLFVVPTLYTYLAEDHRHKPNEPAEIQ"
     gene            complement(8561..9823)
                     /locus_tag="U6309_002134"
     CDS             complement(8561..9823)
                     /locus_tag="U6309_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEN5505572.1"
                     /translation="MNKRLTIMGIILLVVFGGIIAFNLIKAYMIKQFFASYAPPAVTV
                     SSAVAQAVDWKPTIDAVGNFVAINGVDVNSEASGNVVQIHFESGQYVEKDEPLITIDD
                     SIDQALLKFNQSELTLKELSYKRQTDLFKRGATPSSSVDEAKANLQQAQAKVEQIQAQ
                     IKQKHIGAPFSGRLGIRQVNLGQYVTPGQTSIVSLQSLDPLYLEFYLPEQYYKRIHLN
                     QPVTFRVEEFPNILFEGVIHAVNSKVDLTTHNVQVQALLPNCPADAIQDPTKSPLIKV
                     RKQVRGDKLIVSCSTELNKQNKIRNFVFIPGMFASIEVDQPAEPDTIIVPSTAISYSL
                     YGNAVYIIEKSKEGKKNKDGTDQLVVNRVFVTTGEQQGNYTVIKKGIKVGQLVVSTGD
                     LKLQNGTPVVINNSVKLDTDSDPNQLGQ"
BASE COUNT         2930 a         1943 c         1999 g         2981 t
ORIGIN      
        1 aatatcttct atcattctta ttagttcact tttacgagtt ttaatcgata tagagaaaga
       61 taggcaatta atacatcagc ttaagcttaa tgagactagt cgagaacaaa tcaagaatga
      121 acaattggca atgacgaacg ctcgtatgaa agatttaaat aaaataattc ttaatagcgt
      181 ttttttacct attggttttt ttctgttgat cacaactcca atccctttaa ctataatagc
      241 ctgtgtaagt atgttattgg ttcactggtt aattactaat ttaattaata tgaggtattc
      301 accaaatatc tccgtatccc ccactattca aactgctgaa ttatctctct gctgaagttg
      361 agagtgatga tgcttgctaa aaattttaaa ggcgtggctt tgacccaaaa tcaagtatcc
      421 tatctcattt tattctatga aattcattgg tctgcttatt gaattccaaa ccatccgcca
      481 gtatcgccac cggcccagca actgttattt tggcaggcgg ggatcttttc agcacaaagc
      541 tgtttaaatt gataagcagc actgatgtct tgatttttat atccatcagg ttggcaaatt
      601 cgggcgtcat attgcccttt gactcgaatt tgtcccatga ctgaaattgt tggggaaatg
      661 gcgtagacac ctgtattatg cgaataacaa gcaatatagc atcctggata atctacatag
      721 ccgttgtcgg ttggcaggag tacttttttt gctcccgggt aaggatgatt gacaacagca
      781 gacccctcaa tgacgtaaac aggaaaaggt gaagggagca gatctttgtt agaattggta
      841 atattcccag gtatatcaag ggcaaaagag ttcgtaatta ataaaatacc cggaataaca
      901 tacagtattt ttttcattac tattccctcc aaaaaatgcc tttatattgc acatatggga
      961 taatgaaaat ttaatatgtg ctttttatat taaacatacc ttagattata ggttattttc
     1021 atcggaggat ctgagaaacc aatttttacc ttcgaccaga cgtgcaatca tcatgctgca
     1081 aacactattg cttgttacgt tgaccattgt cgctatggga tcaatgatga tgctaatagc
     1141 agcaactgca attaaaacag aaggtggaaa accatacact gacaaaatta acaattctcc
     1201 taacatcccg ccactaggaa tcgctcccat tactgttcct accaacaaag aaacccccaa
     1261 tgccatcata agaacgggca agctggcaaa aggtaaatga aagatcccga ataaaaaggc
     1321 aattttaaaa acaccgccca tgactgatcc atctttatga attatggagc ctaagggtat
     1381 tgatgtttca tagatttcag cagggatgcg catggatttg gttgccatta agttggcagg
     1441 aatactggcg gcactactac aagttgctat ggaggtcgct gcaggaagga aaatatttcc
     1501 ccagaatagc cggacaccat gggattttcc cgccaaataa gcataggcag ttaaggcgaa
     1561 gaaaaagtag aacactgaag cggtatagta gagtatggca atgcgtaaat aattttccat
     1621 taattggggg ccaatttcgc ttactattac tgcaaaataa gcaaaaaaac cgataggagc
     1681 ataatacata atcaggcgaa atactgacat gaatacgtct tctcctgctt ttaagaagga
     1741 attaaaaatt gtacctttat ctttggaatg ggctacagcc aatcctacca gaattgaaaa
     1801 aagaactaat gccagaatat gctcatgaga gaataatttt gaaaactcag gtacagtaaa
     1861 aatctctatc aagcgatccg ggagatttgt cggagagagt tgctttgaac tttccaagag
     1921 aacaggcatg ttttgcgctg gagggaaaag taaaactaca aaaagagcat aaactgcagc
     1981 aactattcct gtaaataaga atatgactgt cattgagtag aatattttgc ctaatttgcc
     2041 aatagaacca gttcttgcaa tcgctgagga tacactgaaa aaaattaggg gaacgatggt
     2101 agtaaaaatc aaattaagaa aaatatcgcc aaagggtttt aaccaggcaa cgttttgatt
     2161 aaaaacatat cctgttaacc caccaagtaa aatagataat agcaaaatga tggggaaaat
     2221 ataggattta agcgtattga attgagttac cacgagaaga catcctttct tttaaataat
     2281 ccggtttgtg ccatttgttc cttacagtca cgtatagaat aatgagttaa gttgagccat
     2341 gactgtaagg agggtttaac taaggatcat tgagttataa tgactcaatc ctgtttttat
     2401 gttgcaaaag tttatcttta gccagttgag cctgagctaa tttgtctttt tctttagcaa
     2461 tgatttcttc aggcgcttta tcagtgaatt tagggttatt taatttaccc tgagctagct
     2521 cgatgtcttt attcaatttg gcaagctctt tattcagtct tgataactct gcctccttat
     2581 caatcaaatc tgccattgga atcaacaact caatttcacc caataccgca gtagctgaaa
     2641 caggaacttt ctcatcagga gcaagataat taattttgtc tatcttgctt agcgtcttga
     2701 gaattttttc atattttgcg atacgctcct ttaattcagg agtaatattg cgtatgtata
     2761 aaggtattaa cttggcgggt gagatagaca tttcactgcg tatggttcgt agtgattgaa
     2821 tggctgactt taaccaatcc aattcttcct ctatagcggg gtttatgaat tcctcattga
     2881 ctttgggata tgtgctcagc atgatactaa tcccattttc actggtaaat ttggtggttt
     2941 tttgccaaat ctcttctgta ataaatggca tcagaggatg aagaagcttc aatatttgat
     3001 ccagaacatg aattaaagtt cttcgcgtgc ctcttttcat ggcgcttaaa gcctgttcat
     3061 cctgtaatat gggcttggat aattctaaat accagtcaca atattcatgc cagacaaatt
     3121 catatagcgt attagcgagg agatcaaacc gataggtttc aaaatagtga tgtaccttac
     3181 ttacggtatt ttgtagccgg gatagtatcc attgatcagc tgggctgtat tgaaatgcgc
     3241 catccccaaa atcaatttgc tcttcgtctg tatttaataa gacatagcga gctgcgttcc
     3301 atagtttatt gcaaaaattt ctatatcctt caactcgtcc taaatcaaaa cgcacattac
     3361 gccctgttga agcaagagaa cagtaagtga atcgcagcgc gtctgttcca taagcgctga
     3421 ttccctcagg aaattcctta cgagtggctt ttgtgattct gtcacgaaca gaatttaaca
     3481 tcaggttaga cgttcgtttg gctattaaag aatccagatc aattccgtct acaatgtcca
     3541 atggatccag tacatttcct ttggatttgg acatcttgtg cccttcactg tcacgaatta
     3601 aaccagttat aaagacttct ttaaagggaa ttttcccagt gaactttagt cccatcatga
     3661 tcatacgggc aacccagaaa aatatgatat caaatccggt taccagtaca gaagtagggt
     3721 aaaattgttc caattcggga gtacgctcag gccagcctaa tgttgaaaag ggccagagag
     3781 ctgatgaaaa ccaggtatcc agaacatctt catcttgttt caggggagta gattgatcaa
     3841 ttttatgttt aaagcgaaca tcgttttcac tataacctac ataaatatta ccatgattat
     3901 cataccacgc aggaattcta tgtccccacc atagttggcg gctgatacac cagtcctcaa
     3961 tattatccat ccactggaaa taggttttat cccatgtctc gggaataaaa cggatgtctc
     4021 ctttctttac agcagcgata gcaggttctg ccaatggttt ggttttaaca taccattgat
     4081 cagttaataa aggttcgatg atgacattcg atttttcacc tcgaggcact tttaatttgt
     4141 gaggttctgt tttcgccagc aagccttcct gttccaaatc tttaatgatt tgttctcggg
     4201 caacaaatct atccatcccc tgatatttta agggagcgtt tttattgata gtccctttct
     4261 ttgttaaaat attaatttgt ggtaattgat gacgtttgcc tacttcatga tcgttaaaat
     4321 catgtgcggg ggtaattttt acacacccac tgccaaattc cttgtcaacg taatcgtccg
     4381 caatcacggg tattgttcta tcacataagg gcagatgaac ctgctttcca ataaggtgtc
     4441 tgaaacgtga gtcatcgggg tgaacggcaa cggcacaatc accgagtaag gtttcggggc
     4501 gtgtagtggc aactattaga aactcttcgg aatcaacaac aggatagcga atgtgccaaa
     4561 gaaagccatc ttcttcttca gagagaacct caagatctga aacagcagtg cccagtttgg
     4621 gatcccaatt caccaggcgg gttcctcggt aaataagtcc ttcttcatag agctgtacaa
     4681 ataccttttg tacagcagca gacaatcctt catccatggt gaaacgttct cgactccaat
     4741 ctacggaagc gcctaaacgt ctcatttgtt gcgtgatggt attaccggac tcttctttcc
     4801 attgccacac tttatccaga aattgttcac gggtgagatc ttttctcgat acgccttggg
     4861 cttccagttg acgttctaca acaagttgag ttgagatgcc ggcatgatcg gtaccgggtt
     4921 gccataatgt tttgtcacca agcatgcgat gatatctggt taatgcatcc attatggtgt
     4981 gttgaaaacc atgtcccata tggaggcttc ctgttacatt aggaggcgga agcataatac
     5041 aaaatcgttt accctcaccg cgtggttgaa agtaatgatg actttcccat tttttataaa
     5101 gggctttttc tattgcttcc ggagagtaag ttttgtccat tatcgtatag cctatactgg
     5161 taaaaactca caattttatc atagagtatg ggaatttagc cattttaaaa aaacaggaac
     5221 tcaggaaaaa aatcattata gtcctttggg ttttaaatgg ccgattctct aatgaccgtt
     5281 gcttgtcgca ggtatccaca aagtacggct gcatgcttcg gagaagacga aacatgcagg
     5341 ctgtttgacg aattatttaa agattaataa ggcttacgaa aaaatccaaa gtcgaattac
     5401 tggatctccg ctggttcgtt tggcttatgt cgatgatctt cagctaagta ggtatataaa
     5461 gtaggtacta cgaacagtgt gaaacaggta cctattaata aacctgtgga gataacgagt
     5521 ccaatgtcga atcgacttgc tgctccagca ccgctggcaa caagaagagg tattactcca
     5581 aacaccatgg cagctgtagt cattaatatg ggtcgtaatc taattccagc agcttcctca
     5641 acggcagcac gtttatcaag atttttttca cgttgcaaat gattagcgaa atctactatc
     5701 aatattccat gtttactgat gagtccaatt aaagtaatca aacccacttg agtgtagata
     5761 ttaatagtcg ctactccaag gttaagcggg ataagcgcgc cacagatgga cattggtacg
     5821 ctgatgagaa cgattaatgg atcccgaaaa ctctcatact gcgctgccag cactaaaaag
     5881 ataacaacaa ttgcaaaaag gaatgcaaag atgagagcac ttccttcttg cataaattgc
     5941 ctggattctc ctccataatc ataagtaaat cccttgggca agacttcctg ggctgctttt
     6001 tgtaaaaatt ccaaaccttg gcctaatgtt ttacctggca tcatgacagc ctgaatcgtt
     6061 gcagaattga gttgttggaa atgggtagct gcattaggtt gagttttttc agttggggtt
     6121 atcacagtgg ataatggaac catagtgccg tttagtgttt taacataaat ttggcctaat
     6181 tgttctatag taagacggaa tttgcgcgcc agttgtggga ttacctggta gcttcgacct
     6241 tccagattga agtaattgat gtagttgccg gatagagcac ttgtcaggct gcttccaagc
     6301 gctctcatat ccaagcccat ttctgaagct tttgcgcgat tgatactaaa ctccacttgt
     6361 ggttggttga atttaaggga attgtctatg taaatgaata acccactatt cctggctttt
     6421 tcagttaact tattcgacac atccattaag ctctggaaat cattcgttgt cttaaccaca
     6481 aattgtacgg caggaccacc acctccgcct ggcaggggag gggggacaat ggcgaaagca
     6541 ttaagtcccg caattttgga taacttgttt tgtaaaggtt gttttagagc aaactgactt
     6601 tttttccttt gatcccatgg tttcaatacc attcctgaaa taggttggct catgttaacc
     6661 gtaaaatagt tttctgtttc aggaaaactt ttataaatgg catcaaaggg ctttgtatag
     6721 gcttctatgt agtctaaggt tgcatattgt ggagcagtag ccattacaaa aaagaagcct
     6781 tgatcttcat caggcgcagt ttcagcagcc gtgttagaat acaaataggg caacattaaa
     6841 atcactaccc ctgctaacag caacatcatt tcccgggtat ccaataagct atgaagtgca
     6901 ttctggtaaa cgcgtttcag tttgttaaaa aactcatcca gaaaaatagc aaattttcca
     6961 ctggtggctt cttcggatag aatttttgaa cacatcatgg gggacagcgt taaggcaata
     7021 acccctgaaa taataaccgc acttgccagc gtaaaagcaa attccttgaa caaagcgcca
     7081 gtcagtccac tcataaaccc tattggtgca taaacagctg ccagggtgat tgtcatcgca
     7141 ataaccgggg tagcaatttc tctggcgcct ataattgcgg attcaaaggg ggtttttcct
     7201 tcttccaaat ggcgatgtac gttttctacc actacaatag catcatccac taccaatcct
     7261 attgctaaca caaaggcaag aagggtaagc aagttgatgc tgtaaccgag tatgagcatc
     7321 aaagtacaga caccaaccaa tgacagcgga atggtgacaa cgggaataag aactgaacga
     7381 acagagccta aaaataaaaa gataacgatg atcacgatga ctgcggcttc aatgatagtt
     7441 tccattacct catggataga tgcccgaatg aatgctgttg catcgtatac tattgtgcca
     7501 gttaatgacg gcgggaattc tctgactatc gaaggaaaca attttctagc atcattgatt
     7561 actgtgagag ggttagccgt tggtgttgga gtgatcgata taaagacggc ctttttactg
     7621 ttgaaagtca ctgagctatc ataattttgt gaacccagtt ccactttagc gatatccctg
     7681 agcctgatta ttgacccctt atcgcttttt acaatcagtc tttcaaattc gccgacattg
     7741 ttcaaatcag ttttagccgt gacattgatg gctacgtatt cacttttagt acttccagcc
     7801 gcagtcaaga aattgttact ggctaaaaca gaggacacat cagcaggagt gacgttaagc
     7861 gcagccattt taattggatc catgaatatt cgcatggaat aagtttgacc acctaaaata
     7921 tcagccttgg ctactccatc cactgtttgc aactggggtt gaacgactcg ggtagcgtag
     7981 tcagtaattt gctgaggagt catatcctta ctgtccagac taatgtacat caatgcggtt
     8041 gatgagtctg attttttcaa tatcaccggt tgttgtgact ctttaggtaa ctgattgagc
     8101 gtttgctgca ctttactcat gacatcagta aaagcaattt gagggtcgaa attcaacttg
     8161 atgtttaagg taattgtgct aaggccttgc tgactggatg aagtcatata atctattcct
     8221 tcagcactgg caacagcact ttcaagagga gtagtgataa atccggctat gagttcagca
     8281 tcagcgccag ggtaaccagt cattatagtt atgactgtat tctccatttt agggtattgg
     8341 cgaatttgca ttttatagat ggagtttatc ccgaatagaa aaatcaacat actgaccact
     8401 atggagagta ccgggcgctt gatgaataga tctgtgaatt tcataagtta tcctgcaaaa
     8461 aactgaccat gatcctagtc ggtagtaaag aacaacaagc catattatta gagcatgcca
     8521 atgttataaa atgatttaaa taccaggttg ccaacacgat ctactgtccc agttggttgg
     8581 gatcactgtc tgtatcgagt ttgacactgt tgttaataac aacaggggta ccattttgta
     8641 atttcaagtc accagtactt acgaccaatt gacctacttt aatgcctttc ttgataactg
     8701 tgtaatttcc ctgttgctcc ccggtagtga cgaatacacg gtttacaacc aactggtcag
     8761 taccatcttt atttttctta ccttctttac ttttttcaat gatataaaca gcatttccat
     8821 agaggctgta agaaatggct gtggaaggga cgataatagt atcaggctct gcaggttgat
     8881 ccacttcaat ggaagcaaac atgcctggga taaatacaaa atttcgtatt ttattttgtt
     8941 tgtttaattc tgtactgcag gacacaatca gtttatctcc tctgacttgt ttacgtactt
     9001 tgataagagg agatttggtt ggatcctgaa ttgcatcagc agggcagttc ggtaataggg
     9061 cttgtacctg aacattatga gtagttagat ctactttaga gtttactgca tgaatgactc
     9121 cttcaaagag aatattagga aattcctcta cgcgaaaggt cacaggctgg ttaagatgaa
     9181 ttcttttgta atattgctct ggcaagtaaa attccaggta aaggggatcc agtgattgca
     9241 aagaaactat ggaggtttgt cctggagtga cgtattggcc caggtttacc tggcggatgc
     9301 caaggcggcc ggaaaaaggg gctcctatgt gtttttgttt aatttgcgct tgaatctgct
     9361 ctactttcgc ctgagcctgt tgcaagttag ctttggcttc atcaacactg gagcttggcg
     9421 tggcgccacg cttgaagaga tcagtttgcc gtttatagct gagttcttta agcgttaatt
     9481 ccgactggtt aaatttgagc aaggcttgat cgatgctgtc gtcgatggtg atcaagggct
     9541 catctttttc tacgtattgc cctgattcaa aatgaatttg aactacattg ccagatgctt
     9601 ccgagttgac atcaactccg ttgattgcaa cgaaattacc tacagcgtca atggttggct
     9661 tccaatccac tgcttgagca acagcagaag aaacggtaac agcaggaggc gcataactgg
     9721 cgaagaattg tttaatcatg taagccttaa ttagattaaa agctatgatt ccaccgaaaa
     9781 ctaccaacaa gattattccc atgatagtca atcgtttatt cattatcttc ccctgttaat
     9841 gcttgaaaaa aat
//
LOCUS       DASLOE010000115         9557 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.116, whole genome shotgun sequence.
ACCESSION   DASLOE010000115 DASLOE010000000
VERSION     DASLOE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9557
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.116"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..733
                     /locus_tag="U6309_002135"
     CDS             <1..733
                     /locus_tag="U6309_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505573.1"
                     /translation="YMEHVNSLENNPTVYFADNNPCVNKDHLEVRTAIEVLKDLCTKD
                     LCPKVFVLDTTSATEAQIEDFLNVFNQQDKIPILVTAASMVKHSEFGLDLWQGGINKA
                     YLSEKSEKNENSQNEFARFIKELKNVTKGTEPGFTRFARRHVREAMNNINEAIKDSQD
                     NSEPQVASCKDDAQNIPNNPISKESNRTEEGHTINRELDEYAPLLTKKGFFAVQIEKV
                     PENNINQNNKQKSFEEGEPPCCTII"
     gene            1160..3142
                     /locus_tag="U6309_002136"
     CDS             1160..3142
                     /locus_tag="U6309_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505574.1"
                     /translation="MNKTLLFVFGSIGLSLNIEISAAKGIVCKDYDGNPMTVKPKTIT
                     IFNNSEVTIYPVLATSKNAVNEWIQGCFRTTESYPTNYVYKLYVNEGTGIVPGSSVKI
                     TLPLYSQLTNDRYITWWNGGRIVLADKNERLRNENDQALSTPEDVSCDGTNTECKLSV
                     YSSDVQFPENIYAQLSEYTFGDSIIPPKQSTRILKPENVGYNISYVDHVYMPVAIGPK
                     NNPYIGYSGSVMSLPTFRSHLNSFLETNLGHGWPIYNLNELKLPGGYNIFAQRSGTLP
                     PGDNVPVKPNDSFPPVLTVLACIQGDCTEEQKASLHFGESVQRMQNLWGSCVNWDEED
                     ISKYITQKVDCPQDLKGKLSAVQQFFKKNHQQYLQLYENGKCNLTPGIDPVPFNYWEA
                     IKHIYGWVPFNEGCGAGANPLAETNIPGWDHARIQSMYIHDLQYNYQNKPILELLFNP
                     YVQLIHDKNYLSMDAYGFSVDDAVGFMSELGDGLIFTVGGTKGLENQHEFNYADGFSV
                     AIGVPLSLLDQVNTPMIKKYGVCVFNQDANDLNCQEVKQNVIMPTNSQIAGFRVGTVA
                     SYPIKVFFTDLNDNLYTFVVHTKFAPCSAEMDPSQCPTNKAEIIDTKSCIVTKSNGEK
                     HPKSNDWCQNANPNQQREKQLTKNFISFPQPVDYME"
     gene            complement(3275..4759)
                     /gene="lspE"
                     /locus_tag="U6309_002137"
                     /gene_synonym="gspE"
     CDS             complement(3275..4759)
                     /gene="lspE"
                     /locus_tag="U6309_002137"
                     /gene_synonym="gspE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE family T2SS ATPase variant LspE"
                     /protein_id="HEN5505575.1"
                     /translation="MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPNTPLQAFA
                     EIKRLLQCELDLKQVDESTFQQHLANIYQSKSSILDAAEGMEEDMDLSMLASQLPVSE
                     DLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
                     IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILD
                     KQATQLDLNLLGMPEHTLKTVRQMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTR
                     NILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVVMIGEIRDLETAEIA
                     VQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKT
                     PHELRHEEKELMGLKPDADVSKVFEPKGCDSCNHLGYKGRTGIYELIIVDETLRGMIH
                     RNENIQTMENYLRPTTPSIREDGFKRVLCGDTSLAEILRVTSQN"
     gene            complement(4763..7138)
                     /gene="lspD"
                     /locus_tag="U6309_002138"
                     /gene_synonym="gspD"
     CDS             complement(4763..7138)
                     /gene="lspD"
                     /locus_tag="U6309_002138"
                     /gene_synonym="gspD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspD family T2SS secretin variant LspD"
                     /protein_id="HEN5505576.1"
                     /translation="MRSIVIILLAFVCCSLVRANDVNISTVTKDGNTFFYLQAGAYNL
                     EKDAKIRQNELAKLVDQKVEIKNLADKHLYLVQIGPIDDYQTARALKEKLSQKTEKQF
                     NKISKNQTSSLEVSQLPKNMEQAQTAPPGSKLWNLRNADIRAVIAEVSRITGKNFVID
                     PRVQGKVSIVSSTPLSSRELYQVFLSVLQVSGYAAIPNGEIIKIIPNIDAKTQSPDLL
                     SGMKSPPRGDDMMVAVIPVHYVPSEQLVPVLRPLMPQWSSVSAYAPSNMLILSGRANN
                     IKSLVEIIKQVDSSSANGIDMVRLHHALAMDVANTLKDLVKTQPGIGSRTQITIAADD
                     RSNSILVSGSKTDRIRLRMLILKLDKDSSTGVNSNTQVVYLNYLRAEDLVPILAGIAQ
                     ANFSGNVGTTIGTITRPALDSTNPASSLANTSADGQSSSLSSTSTSSAPMNASGATAN
                     TTSASTQNEGSTKPTVQIIGEPNTNSIILNAPASIIRILKTVISQLDIKPAQLLIEAL
                     VAEVDEKDVNNLGIEWGSNQQTGNPKDFRPGFAIINSKTRIDDFQAQIYALAREQRAN
                     ILSTPSVVVLDNRQAKILIGKQVSIATTSYPNNAGGTTTASPFTTFDRVNVALHLYVR
                     PQITRGQGIQLQIDQGNDTLDPATATTDNTTTPTFNISSIVTSVHVESGDIVVLGGLI
                     QDSIGNDNNKLPILGDIPGIGRLFQRNIRNRDKRVLMVFIKPIILRNERDNLHVTGEK
                     YNYVRQYQLDWIRSQEAFEQTDDETVLPPLTQGNLPVPFSTPPQYSSKMTK"
     gene            7311..8339
                     /locus_tag="U6309_002139"
     CDS             7311..8339
                     /locus_tag="U6309_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN5505577.1"
                     /translation="MDNATIKWGILGTSFISEVMANAIQESKTSELVAIGSRTSEIAK
                     RFSEKFAIPKFYDNYQSLILDSDIDVVYIGLPNHLHKEWIIRCAQAGKNILCEKPFVI
                     GIEEIHEVISAIERTNVFCMEALMYRYHPFIKKLQEIIQSSTIGKIKLYNATYTANIA
                     EIANPVAGGSIRNLGCYPISLVRLLANAEPIEIRGIGRMNSRNNMDSQASVLLKFEDD
                     SIATVSTADDIEMHWQFEIYGTKGHLKVETNPWLPGCEGNKIYISLNTESTPREIGVK
                     AEKSLYTYQIDFINEQVLKGDSRQFSKTSLLDSMGNTIVLETWLKQIIPVKPSFELSR
                     LKDRLPHE"
     gene            complement(8360..>9557)
                     /locus_tag="U6309_002140"
     CDS             complement(8360..>9557)
                     /locus_tag="U6309_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEN5505578.1"
                     /translation="AYQPISSTNEKALWHIIIIVPETDVIEPLKDLSMRYILLTILVL
                     LIGTLLVRIVSQRISNPIIQLTEEAKEITMLNLKPRPLLKTRIKEVSYMDKTLSSLRS
                     SLASFQRYVPGSLVKKLMRSGKIAQVGGQSQTITILFSDIKDFTSISESTSPQKLMTY
                     LSDYFQSMTEIVIQHEGTLDKYIGDAIMALWNAPSKDTNHALHACLAAKIMIERLSLL
                     NQKNKHLGFPEFKIRIGIHTGDAIVGNVGSEDRLSYTALGDSVNLANRLESINKNYHT
                     QIIVSHATFTQVSEKFPFRLLDKVAVRGKRESIEIYELITAQNLENLERHKKEFQKAF
                     SLYQKGSWKESISAFAKLTPAYSGDPLASVYIERCKVLAVNTPVNWDGIWREGKTDYS
                     LLGKFD"
BASE COUNT         2919 a         1679 c         1903 g         3056 t
ORIGIN      
        1 ttatatggag catgtaaata gcctggaaaa caatcctaca gtgtattttg ctgataacaa
       61 tccttgtgtt aacaaagatc atctggaggt aagaactgcc atagaagttt taaaagatct
      121 ttgcactaaa gatctttgcc caaaagtgtt cgtcctggac acaacttctg caactgaagc
      181 ccaaatagaa gattttttaa atgtgtttaa tcaacaagat aaaattccaa ttcttgtgac
      241 tgctgcaagt atggttaaac acagtgaatt tggattagat ttatggcagg gaggaatcaa
      301 caaagcctat ttgtcagaaa aatcggagaa aaatgaaaat tcgcaaaatg agtttgcacg
      361 ctttataaag gaactcaaaa atgttaccaa aggaaccgaa ccaggcttta cacgattcgc
      421 aagacgacat gttcgagaag caatgaataa tataaatgag gcgataaaag attcacaaga
      481 taatagtgaa cctcaagttg catcatgtaa agatgatgcg caaaacatac caaataaccc
      541 tatttctaaa gaaagcaata ggacagagga aggacatacg attaatagag agcttgacga
      601 atatgcgccc ctacttacaa agaaaggatt ctttgcggta caaattgaaa aggtgccaga
      661 aaacaatatt aatcaaaata ataaacaaaa atcttttgag gaaggcgagc cgccttgctg
      721 cacaataatt taacttccaa gattttgttt ttatttgtag tcgttcaaat ttgatctgag
      781 ggttgcaggt ttttcaagaa gcacttctca gatcaaatta tctaatcctt ttcggcataa
      841 aatgttaatt acatctccaa tatgtaactg atttcttcat aatagacact gacactcccc
      901 attactgcgt agtgccatcg tagttgaagc atgctttgat tgtagaggtt tatattaagt
      961 taaatcaata cggttcatta ttgggaaata aagatcttca tattaactcc tttaatttga
     1021 taatgattag tatgtaagaa tataaattct agctggtgta taggctaata ccataatttg
     1081 ctcacctaac agatgattgt taatttaaaa attacttttt gtgctatttt tataaaatat
     1141 tgtacaaaga gatattaata tgaataaaac actgcttttt gtctttggaa gtataggttt
     1201 gtcattgaat atagagattt ctgctgctaa aggcattgtg tgcaaagact acgatggcaa
     1261 tccaatgaca gtcaagccaa agacaatcac tatatttaac aactctgaag ttacgattta
     1321 tcctgttttg gcaaccagca aaaatgcagt aaatgaatgg attcaaggct gttttcgtac
     1381 cacagaatct tatccaacaa actatgttta taaattgtat gtgaacgagg ggacagggat
     1441 agttccaggt tcctcagtaa aaattacctt gccattatat agtcaattaa caaatgatcg
     1501 ttacatcact tggtggaatg gagggcgtat cgttttagct gataaaaacg aaagattgag
     1561 aaatgaaaac gatcaggcat tatcgacccc tgaagacgtt agctgtgatg ggacaaatac
     1621 cgagtgtaaa ttaagcgtat attccagcga cgttcagttt ccagaaaata tatacgcgca
     1681 gttatcagaa tatactttcg gcgactctat tattccaccc aagcagtcaa caagaatatt
     1741 aaagccagaa aatgtaggct ataacatttc ctacgtagac catgtttata tgcctgttgc
     1801 aatagggcct aaaaataatc cttatattgg atatagcggt tcagtaatgt ccttgcctac
     1861 atttagaagc catcttaatt cgtttctaga gacgaattta gggcatggtt ggcctattta
     1921 taatctcaat gaattaaaat taccaggagg ttataatatt tttgcacaaa gatctggcac
     1981 attacctcct ggggacaatg tacctgtcaa gccaaatgat agttttccac cggtattaac
     2041 agtgttagct tgcattcagg gggattgtac cgaggaacaa aaggcatcac tccattttgg
     2101 tgagtcagtt cagcgtatgc agaatttatg gggttcttgt gtcaattggg atgaagaaga
     2161 catcagcaaa tacataaccc aaaaagtgga ctgcccacaa gacttaaaag gaaagcttag
     2221 tgctgtacag caatttttta aaaaaaacca tcaacaatat ttgcagctgt atgaaaatgg
     2281 gaagtgcaat ttaacccccg gcatagatcc tgtgccattt aattactggg aagcgattaa
     2341 gcacatatat ggttgggttc cttttaacga agggtgtggt gctggcgcta atccattggc
     2401 agagacaaat atcccaggat gggatcatgc taggattcaa tccatgtaca ttcatgattt
     2461 acagtataac taccaaaata agccaatact tgagttatta ttcaatcctt atgtccaatt
     2521 aatccatgat aaaaattatc tttccatgga tgcttatggg ttttctgtcg atgatgctgt
     2581 tggctttatg agtgagctag gggatggatt gatttttact gttggtggga ctaaaggtct
     2641 tgaaaatcaa cacgagttta attatgcaga tggtttttct gtggccattg gtgtgccatt
     2701 atctttatta gaccaagtaa atactcctat gattaaaaag tatggtgtat gtgtgttcaa
     2761 tcaagatgcc aatgacttga attgccagga agtaaagcaa aacgttatta tgcctactaa
     2821 tagtcaaata gcgggattta gagtaggtac tgtagcttca tatcctatca aggtattttt
     2881 cactgatttg aatgataacc tgtatacctt tgtcgtccat acgaagtttg ctccatgctc
     2941 tgcagaaatg gatccatctc aatgtccaac gaataaggca gaaattatcg atacaaaatc
     3001 ttgtattgtt accaagagca atggagaaaa gcatcctaag tcgaatgatt ggtgtcaaaa
     3061 tgctaatcca aaccaacaaa gagaaaagca attgactaag aatttcatca gtttcccaca
     3121 gccggtagat tatatggagt aggtgctgaa atataatgcg actttagaac ttcttcttag
     3181 gctaattact ttgtttaagc aagctgatta attaaatgat taatatcttg aagacgtggt
     3241 gtgactgttt aaaaattgct aaggtgtggg taatttagtt ttgagaggtt actcttaaaa
     3301 tttctgcaag tgacgtatcg ccacataata ctcttttaaa accatcttca cgtatgcttg
     3361 gtgtggttgg tcgtaaataa ttttccattg tttgaatatt ttcattacga tgaatcattc
     3421 ctctcagggt ttcatctacg ataattaatt cataaattcc tgttctgcct ttataaccca
     3481 agtggttgca tgagtcgcat cctttgggtt caaagacttt agatacatca gcatcaggtt
     3541 taagacccat taactccttt tcttcatgtc ttaattcatg aggtgttttg caatgagagc
     3601 ataatttcct taccaaacgt tgcgcaatga gcccgactat actggatgac aataaaaaag
     3661 actcaactcc catatcatgc aggcgagtta atgctcccaa tgcggtattg gtatgaaggg
     3721 ttgatagtac caaatgccct gttaagctgg cttgaacagc aatttcagct gtttccaaat
     3781 cacggatctc acctatcatg acaacatccg gatcttgacg taatatggcc cttaatcctt
     3841 tggcaaaagt catttgaact ttagtattaa cctgcgtttg tcctacacca ggcaggtcat
     3901 attcaattgg atcttcaatg gttaaaatat tacgagtcac ctgatttagt tcggtgagca
     3961 tagcatagag agaggttgtc ttgcctgaac ctgttgggcc agtgactaaa ataatgccat
     4021 gaggttcagc aatcatttgt cgtactgttt ttaatgtatg ttcaggcata cccaaaagat
     4081 ttaaatccag ttgagtagct tgtttatcca atatccttaa aaccactcgt tctccatggt
     4141 ttgagggtaa agttgagacc ctgacatcaa tattgtgtcc acctatacgc aatgaaattc
     4201 gaccatcttg aggaattctc ttttcggcga tatccaattt ggccatcacc ttaactctgg
     4261 aaatgaccaa aggagcgata gctcgttgaa tttcaaggac ctcttgtaga acaccatcaa
     4321 ttcgattgcg caccaatact ctgtcttcat aagtttcaat gtgaatatca gatgcttttt
     4381 gtttaatcgc ttgggtaaac agagcgttca gtaagcggat aattggggca tcgtcttggt
     4441 tttccaataa gtcttcactg acgggaagtt gactggccaa catggataaa tccatatctt
     4501 cttccatgcc ttctgcagca tccagtattg aagatttaga ttggtaaata ttggctaaat
     4561 gttgctgaaa agtggattca tcgacttgtt ttagatcaag ttcacattgt aataatcgct
     4621 ttatctcagc gaaagcttgc agtggtgtgt taggtaagtg atagacaata gccagatttt
     4681 cttgtatttc tcccacaaca attccattgc ttttggcaaa actataagga attttaagtg
     4741 atttttcttc attttccatg atttatttgg tcatttttga tgagtattgg ggaggagtac
     4801 taaaaggtac tggtagattg ccttgagtta atgggggtaa cactgtttca tcatctgtct
     4861 gttcaaaagc ctcttgggat cgaatccaat ccagctgata ttgacggaca taattgtatt
     4921 tttcgcctgt gacatgcaag ttatctcttt cgttgcgtaa tatgataggt ttaataaata
     4981 ccataagcac tcgcttgtct ctgttgcgaa tgttacgttg aaacaatctg cctattccag
     5041 ggatatctcc taaaatgggt aatttattat tatcatttcc aatactgtcc tgaatcaacc
     5101 ctccaagtac tacaatatca ccactttcta catgaactga agttacaata ctgcttatat
     5161 taaaagtggg ggttgttgtg ttgtctgttg tagcagtcgc tggatccaaa gtatcatttc
     5221 cttgatcaat ctgtagttga attccttgtc cgcgagtaat ttgcggcctt acataaaggt
     5281 gcaaagcaac gttaaccctg tcaaatgttg tgaaaggact cgctgtggta gtgcctcctg
     5341 cattattggg gtagctggtt gttgctattg agacctgttt acctatgagt atttttgctt
     5401 gacgattatc aaggactact acggagggag ttgataaaat atttgctctt tgctctcttg
     5461 ctaaggcata aatttgagct tggaaatcgt caatcctggt tttactattt ataatggcaa
     5521 agcctggtct gaaatccttg ggattgcctg tttgttgatt tgatccccac tctattccta
     5581 gattgttaac gtccttttca tccacttcag caacgagggc ctcaattaat agttgagctg
     5641 gttttatatc tagctgagaa atgactgttt ttagaatgcg aataattgaa gcaggtgcat
     5701 taagaataat agagttagtg ttaggttctc ctattatttg tacagtaggc ttggtagagc
     5761 cttcattttg agtacttgct gatgtcgtat ttgctgttgc tcctgacgca ttcatagggg
     5821 cagaactggt cgaagtggag cttaatgaag agctttgtcc atcggcagat gtattggcaa
     5881 gactggaagc agggttggtg ctgtctaatg cgggtcttgt aatggttcca atggttgttc
     5941 ctacatttcc actgaaattg gcttgggcta tacctgccag tatagggact aaatcttcag
     6001 ctcttaaata attcaggtaa acaacttgtg tatttgaatt gactcctgtt gagctgtctt
     6061 tatccagctt caatatgagc attcgcaaac gaattctatc cgtcttgctc ccactgacta
     6121 aaattgagtt ggaacggtca tctgcagcaa tggtaatttg tgttctgctg ccgataccag
     6181 gttgagtttt aaccaggtct tttaaagtgt ttgcaacatc catggccaaa gcatgatgta
     6241 aacgaaccat gtctatgccg tttgcacttg agctatccac ttgtttgata atttccacaa
     6301 ggcttttaat attgtttgct cgaccagaca ggatcaacat attagaagga gcataggcag
     6361 atacgctgct ccattggggc attagtggtc ttagaacagg taccagttgt tcagaaggaa
     6421 cataatgaac gggtattacg gcgaccatca tatcgtcacc tcgtggagga ctcttcattc
     6481 cactgagtaa atcaggtgac tgtgtttttg catcaatatt gggaataatt ttgataattt
     6541 ctccgttagg tattgcggca taacctgata cctgcagtac tgagagaaag acttgatata
     6601 gctcgcggct ggataacggc gttgatgaaa caatcgatac ttttccttgt actctgggat
     6661 caataacaaa atttttacct gtaattcgag agacttctgc gattacggct ctaatatcag
     6721 cgttacgtaa attccataat ttactgcctg gaggagccgt ttgcgcttgt tccatatttt
     6781 taggcaattg gcttacctca agtgaagagg tttgattttt gctaatttta ttaaactgtt
     6841 tttctgtttt ttgcgataac ttttctttga gtgctcttgc agtttggtaa tcatcaattg
     6901 gaccaatctg cacaaggtaa agatgtttgt cagccaggtt tttaatttca actttttgat
     6961 cgactaattt ggcaagttca ttttggcgta ttttagcgtc tttttcgaga ttataggcgc
     7021 cagcctgaag ataaaaaaat gtattgccat ctttggttac ggttgaaata ttgacatcat
     7081 tagcacgtac taatgaacaa caaacgaagg ctaaaagtat aattactatc gagcgcattt
     7141 aaaaaccgta aaataatgga taaagacata ataactaaat ttgactgctt accatagctt
     7201 ttttaataac ttagagcagg aaatttaagt ggttttaact gctatggttt tactcgtgtc
     7261 atttatgcgt tttaatatac agtcagcaat tttttagccg gaaggaatat atggataatg
     7321 cgacaataaa gtggggaatt ttaggaacaa gttttatctc tgaagtgatg gctaatgcaa
     7381 ttcaggaatc aaaaaccagt gagcttgttg ctattgggag tcgaacaagc gagatagcca
     7441 aacgattttc tgaaaaattc gctattccaa aattttatga caattatcaa tcgttaattc
     7501 tcgatagtga tatagatgtg gtttatatag ggttgcctaa tcatctgcat aaagaatgga
     7561 ttattcgttg tgcgcaggca ggaaaaaata ttttgtgtga aaaacctttt gttattggta
     7621 tagaagaaat ccatgaggtt atttcagcaa ttgagagaac aaatgttttt tgtatggaag
     7681 ctctaatgta ccgatatcat ccctttataa aaaaattaca agagatcatt caaagcagca
     7741 caataggtaa gataaagttg tataatgcga catacacagc aaacattgca gaaatagcca
     7801 atcctgttgc gggagggagt atacgtaatc tcggatgtta cccaatttcc ttagtgaggc
     7861 tgcttgctaa cgcagagcca atcgaaatcc gtggtattgg aagaatgaat tccaggaata
     7921 atatggatag tcaagccagc gttcttttaa aatttgaaga cgattcaatt gcaacagttt
     7981 ccactgccga tgatattgaa atgcattggc aattcgagat ttatggaaca aaaggccatt
     8041 taaaagtaga aacgaatcct tggttgcctg gctgtgaggg taacaaaatt tatatttctc
     8101 tcaatactga atcaacaccc agagaaatag gcgtgaaagc agaaaagtca ctttatacct
     8161 atcaaataga ttttatcaat gagcaagtat tgaaagggga ttccagacaa tttagcaaga
     8221 cgtctttatt agatagcatg ggaaatacaa tcgttctgga aacctggcta aaacaaataa
     8281 tccctgtaaa accaagcttt gaattatcca gattaaaaga caggctgcct catgaataag
     8341 caaaatggtt ctggtgatac tagtcaaatt ttccgagaag agagtaatct gtttttcctt
     8401 cgcgccagat accatcccaa ttgacaggtg tattaactgc taataccttg cagcgttcga
     8461 tgtaaactga agctaatgga tcaccagagt aggctggagt aagttttgca aaagcgctta
     8521 tactttcttt ccaggaacct ttttgataga gagaaaatgc tttttgaaat tcctttttat
     8581 gtcgctcaag attttcaaga ttttgggctg taatcagttc atatatctca atactttcac
     8641 gcttacccct tacagctact ttatctaaca aacggaaagg aaatttttca gatacttgag
     8701 taaaagtggc gtgactgaca atgatttgag tatgataatt tttattaatt gattccaggc
     8761 gatttgccag attgactgag tcgcccaagg ctgtataact tagcctgtcc tctgaaccaa
     8821 cattacccac aatagcatct ccggtgtgaa taccaattct gattttaaat tcgggaaatc
     8881 caagatgttt attcttttga tttaacaggc taaggcgctc aatcatgatt ttggcagcca
     8941 ggcatgcatg caatgcatga ttggtatctt ttgaaggtgc attccataat gccatgatag
     9001 catcgccaat gtatttatcc aaagtccctt catgttgtat tactatctcg gtcatggact
     9061 ggaaataatc ggataaatag gtcattaatt tttgtggtga ggtggattct gaaatacttg
     9121 taaaatcctt tatatctgaa aataaaatag taattgtttg gctttgccct ccaacttgag
     9181 caatctttcc gctacgcatt aattttttta ccaaagatcc gggaacatag cgttggaatg
     9241 aagctaagct tgatcgcaga gatgatagtg ttttatccat ataactgact tctttaatcc
     9301 tggtcttcag aagtggcctg ggtttgaggt taagcatggt aatttcttta gcttcttcag
     9361 tcagttgaat aataggattt gatattcttt gtgaaacgat acgaactaat aatgtcccaa
     9421 tcagcaagac cagtattgtt aacaaaatat agcgcatgct taagtctttc agtggttcta
     9481 tcacatccgt ttcaggaaca ataataataa tatgccacag tgctttttca tttgtactgg
     9541 atataggttg gtatgct
//
LOCUS       DASLOE010000116         9523 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.117, whole genome shotgun sequence.
ACCESSION   DASLOE010000116 DASLOE010000000
VERSION     DASLOE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.117"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(157..924)
                     /locus_tag="U6309_002141"
     CDS             complement(157..924)
                     /locus_tag="U6309_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN5505579.1"
                     /translation="MNPIKPPFTLNQYFAAWFVHVFTASAACIGVFSLYKIYQHDYVF
                     ALWLMAITVFIDAVDGSLARLVHVKSVLPKIDGALLDNIVDYLNYVITPCFFLLVKPG
                     MLPADYVVPIIAAITITSAYQFCQDDAKTPDHFFKGFPCYWNITVFYMYIFNTSMIVN
                     TVLLSLFCVLIFIPVKYVYPSRLDYLTESRILKILMHCCSALYGISSFCLLVNYPETN
                     KLWVSLSLGYVGMYLFLSFYRTYYPMFKAKITANNKD"
     gene            1266..2654
                     /locus_tag="U6309_002142"
     CDS             1266..2654
                     /locus_tag="U6309_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEN5505580.1"
                     /translation="MNIQTVNPTTEEVLNNYTCLDFDSIDKKIACGYEAYLNWKNSKF
                     DIRRSLMLNLAQILKDKKQELAHLMALEMGKPITAGRAEIEKCAWVCEHYAENAEQYL
                     MPRTVQTEMKTAKVCYRPLGIVFAIMPWNFPFWQVFRFSAPTIMAGNGAILKHAPISS
                     GTGNKIEELFRDAGFPSSLFQHLIVDNDGASHIIENQFVTAVTLTGSERAGQSVAAHA
                     GKNLKKSVLELGGSDPYLILEDADLDLAAQCIVASRLNNTGQVCIAAKRVIALQSIEK
                     ELISKIKELISKYKMGDPLDPNINLGPMARKDLRENLHSQVEKSVKQGAKLLLGGIIP
                     EGKGYFYPPTLLTDVKPGMTAFDEELFGPVIVIITAKNESEAISLANMSQYGLGAAVF
                     TKDLNKGEHIATYEIEAGTCFVNAFVASDPRLPFGGIKRSGYGRELSREGILEFVNTK
                     TIAISDPKHGKK"
     gene            2641..3129
                     /locus_tag="U6309_002143"
     CDS             2641..3129
                     /locus_tag="U6309_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEN5505581.1"
                     /translation="MVKNSKFIFISIKKQEMSCFDKDHFIANYAISSGKNGVGEKINS
                     GCTPRGWHKIHSILGIDLPINSVMVAREWTGEIYSESLATQFPERDWILTRILQLDGL
                     EPGRNKGGDVDTLQRYIYIHGTPDTTQLGIPGSHGCIRLNNDAVIELAAWATKNTLVY
                     IQ"
     gene            3181..8058
                     /locus_tag="U6309_002144"
     CDS             3181..8058
                     /locus_tag="U6309_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEN5505582.1"
                     /translation="MSYKFEEGKDAIVKSVVEKIKQTMTGEQTEFCAEFAKQFYGTVA
                     LEDLLEWEVDDLYGAAVNFWSLICERAPHETKIRIYNPDYERHGWQTTHTVVEVICED
                     MPFIVDSLRIVINRMGLTSHLTIHMGGIRVKRDNHNRVTEILPRNGGSAGDDILHEAP
                     IFIEIDRQTDPASLSELHKNFERALEDNRAVFEDWDKMRTKVREIIKELDTVPKTIDI
                     SEIEETKAFLNWMEDHHFTFLGLRDYDLVKKGKETILQPIPETGLGVLRQSLSKSNAR
                     SITAMGPEAQGLISSPRILVMSKTNTLASVHRDAYTDYIGVKRFNKKGEVIGERRIIG
                     LYTSAAYHTNPKHIPFLRHKVALIMKNSNLNPYSHAGKVLLNILETLPRDDLIQGTED
                     ELLEIAMGIFYMQDRKRIRLFARADVYKRFISCLVYVPKDRFNTELRYAMQKILADSF
                     NAEEITFSTQFSESVLARIHFIVRVNPKNLPDFDLKEIEQKLIEVGRSWIDDLQHHLH
                     EVYGEEQANALYSRYKNAFPISYSDTFSSRTAVYDIKHIEMLSPENPLGINFYKPLDE
                     SEKSFRLKVYQHDTTIPLSDVLPILEKLGLRAISERPYVLKFEDGKVAWINDFAMQYN
                     KESEFNIDEIKELFQNAFARVWFGDAENDGFNLLVLAAGLNWREVAVLRTYAKYFRQI
                     GFTFSQDYMETALNNNVGIAKKLVRLFEIRCNPHDSKRREDRYVALVAEILSDLDNVA
                     NLDEDRIIRQYVHAIGATLRTNFYQVDAQGNPKNYISIKLSSKLIPGVPKPYPMFEIF
                     VYSPRFEGVHLRCGKVARGGLRWSDRREDFRTEILGLMKAQQVKNSVIVPSGAKGGFV
                     PKQIPANATREEIMEEGISCYKLFIRGLLDITDNYKEGLIVKPQNVVFYDEDDPYLVV
                     AADKGTATFSDIANSISQEYDFWLGDAFASGGSVGYDHKKMGITAKGAWESVKRHFYE
                     LNRDIQNNDFTVVGIGDMAGDVFGNGMLLSRHIKLVGAFNHIHIFVDPNPEAEASFKE
                     RERLFNLPRSNWTDYDKKLISKGGGVFSRSAKSIPVSPEMQKVFGIKQTSIEPNDLIK
                     AILKADVDLLWSAGIGTYVKSSTESNTSVGDRTNDATRVNANQLRCKVIGEGGNLGLT
                     QLARVEYSLHGGKVYTDFIDNSGGVNCSDKEVNIKILLNNVVSAGDLTPKQRNELLSN
                     MTDEVAKLVLRDNFLQTRAISLTASQALRAIELHARYINELEKTGKIDRTLEFLPDEK
                     VLMEHKLMGKGLTQPGIAVLMCYSKTILKEQVLASEVPEEDYMNQILIGSFPKPLQER
                     FSKQMQDHPLRREIIATRLSNIIVNEMGFTYVYRLQDETGASVAAIVRAYMIARSVLN
                     LEAIWKQIEELGTKISAQAQVDMMMLYVRLSRRVTRWFLRTHRRSMSITQAIELYAKG
                     VDELKKSMPAVFGETGRIQYEEHYQERIKEGIPPQLAHELTVTRGLFAATDIIEIAYE
                     ENIKIPKVAEIYFGIGEFLDIAWLRTQIIVHTTENHWESLSREALRDDLDWQQRQLTA
                     AIMGFEPNNKDLQERLTRWGETHVSLIDRWNYILTALKSSTALNYTMFLVATRELLDL
                     TQTTLQSNLKMEESF"
     gene            8079..8627
                     /locus_tag="U6309_002145"
     CDS             8079..8627
                     /locus_tag="U6309_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEN5505583.1"
                     /translation="MEAKTASEYSTLSKLFHWIIALAVIIMLIAGFFLDEIPEEFQGV
                     AYMLHKSTGITILFLMILRFIWIHAKGKPALPASVKSWEKILSRFVQYGFYVLLIVMP
                     LSGWIMSVAADRIPNYFGLFKAPLPWIEPNKSLAKLMAESHEIIAWVLIAFITLHVLG
                     ALKHHFIDKDNVLKRMLPGKSK"
     gene            complement(8658..>9523)
                     /locus_tag="U6309_002146"
     CDS             complement(8658..>9523)
                     /locus_tag="U6309_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEN5505584.1"
                     /translation="DLFGEKSIDFLQGQLTCDLRLVSDTSMIQGAQCNLKGRILSLLD
                     IINWQGVKLVLPQDLIEVTQNSLNKVALLSRVKITSNHNYKILGFYLQHGNDQIPQLL
                     PLPSELYALSCTSHGCVYHLGKGFYIYLIHSDYYDSLCKPFIENNQLLGSLTWHTLRL
                     FNNQIDIYPNSRGLFLPHRIGLHQTAYVSFDKGCYKGQEIIARTHYRATLKHELKKFV
                     IQSDNQLYSGQKLFKSDEDTEIGELIDYSMLGENRYLAAASILKENFSSVCLEGNKFP
                     IQLEEKIELEL"
BASE COUNT         3217 a         1562 c         1918 g         2826 t
ORIGIN      
        1 tagatcttat atcaatagct tgcattacct ctctattttt tcagtaaaca gatactattt
       61 aaagcaataa gaaaagaaaa attcctaaaa gagtacgata aaacagtgcc accagagatc
      121 taatatctgc atggaaaaca taaattacat ataaactcaa tctttattat ttgccgtaat
      181 cttagctttg aacatcggat aataagtcct gtagaaactt aaaaataaat acatgcccac
      241 atagcccaaa gataggctta cccataattt atttgtttca gggtaattaa ctagcaggca
      301 aaatgaactg atgccataaa gagcagaaca acaatgcatt aatattttca agattctcga
      361 ctcagtcaag taatctaacc ttgatgggta aacatatttt actggaataa aaatcagtac
      421 acaaaaaagg gacaagagga cagtgttcac aatcatagaa gtgttaaaaa tatacatata
      481 gaagacagta atattccaat agcaggggaa ccctttaaaa aagtgatcag gggttttggc
      541 gtcatcctgg cagaattgat aggccgaagt aattgtaata gcagctataa ttggcacgac
      601 ataatcagct ggcagcatac ccggtttaac tagcaagaaa aaacatggag taatgacata
      661 attcaaatag tcaactatat tgtctaataa agcgccatca attttaggca atacgctttt
      721 tacatgaacc aatcgagcaa ggctaccatc tactgcgtca ataaatacag taattgccat
      781 tagccataaa gcaaatacat aatcatgctg gtaaatttta tataagctaa agacaccaat
      841 acaggcagcg ctggcagtaa atacatggac aaaccaggcg gcaaaatact gattaagggt
      901 aaagggaggc tttattggat tcattactta tcctagagtt aatcaatgtc atctactact
      961 aattagggct tcagtttttt tgtaaaagca aaaaacaaat tacctttaaa aatatccagt
     1021 gcaatgatta aatctggatt gctttgttca tcaagacgat gtgtttttac tttttactaa
     1081 aacagtgcaa gtccttttta cttaacaggt tgatttttat tactgttctt taagacaatg
     1141 tcgtttaaaa taaaaatagt atcaatattc aaaagcaaag tgcaagttag gttaattatt
     1201 taaaataaaa aaaagccttg atttggtcta aactataggc aacgttaatc atatcgagta
     1261 attaaatgaa tattcaaact gtcaacccga caactgaaga agttctaaat aactacacat
     1321 gcttggattt tgattcaata gacaaaaaaa tagcgtgtgg atatgaggcc tatttaaatt
     1381 ggaaaaattc taaattcgac ataaggcgct ctttaatgct caatcttgct cagatattaa
     1441 aagataaaaa gcaagagtta gcacatttaa tggctttaga aatgggtaaa cccattacag
     1501 caggtcgtgc tgaaattgag aagtgtgctt gggtatgtga acattatgct gaaaatgcag
     1561 aacaatacct aatgccaaga actgttcaaa ctgaaatgaa aacagcaaaa gtttgttaca
     1621 gaccactagg aattgtattt gccataatgc cttggaattt cccattttgg caggttttta
     1681 gattttcagc accaactatc atggcgggta atggggctat tttaaaacat gccccaatat
     1741 cgagtggaac aggaaataaa atcgaagaat tattcagaga cgctggattt ccttcttcat
     1801 tatttcaaca cttaattgta gataatgatg gggcatctca tatcattgaa aatcaatttg
     1861 ttaccgctgt tactttaact ggaagtgaac gtgcggggca aagtgttgca gcgcatgcag
     1921 gtaaaaattt gaaaaaatcg gttctagaat taggcggttc agatccctat ttgattctgg
     1981 aagatgcgga tttagatctt gctgctcaat gcattgtggc ttcacgatta aataataccg
     2041 ggcaggtctg cattgcggcc aagcgagtta tagcacttca atcgattgaa aaagaattaa
     2101 tttctaaaat caaagaactc atcagcaaat acaaaatggg agatccatta gaccccaata
     2161 ttaatttggg acctatggca agaaaagatt tacgagaaaa tttgcattca caagtagaaa
     2221 aatctgttaa acaaggtgct aaattattgc ttggtggtat aattccggag ggtaaagggt
     2281 acttctaccc gccgacatta ttaactgatg ttaaacctgg aatgacagct tttgatgagg
     2341 aactatttgg gcctgttatt gttattatta ctgctaaaaa tgagagtgag gcaatttcac
     2401 tggccaatat gagtcagtat ggtttaggtg ctgctgtatt tacaaaagat cttaataagg
     2461 gagagcatat tgctacttat gaaattgaag caggaacctg ttttgttaat gcttttgtag
     2521 cctctgatcc aagacttcca tttgggggaa taaagcgatc aggatatggt agagagttat
     2581 caagagaagg tatattagag tttgtaaata ccaaaacaat tgcgattagt gacccaaaac
     2641 atggtaaaaa atagtaaatt catttttatt tcaataaaaa aacaggagat gagttgtttt
     2701 gacaaggatc atttcattgc aaactatgct atttcatccg ggaaaaatgg cgtgggtgaa
     2761 aaaattaata gtggatgcac cccgagaggt tggcataaga tccattctat actcggcatt
     2821 gatctgccaa ttaatagtgt gatggttgcc agagaatgga ctggtgagat ttattcagaa
     2881 tcattggcaa ctcaatttcc agaaagggat tggatattaa ctcgaatttt acaattggat
     2941 ggattagagc ctggtcgaaa caagggcgga gatgtagata ctttacagcg ttacatttat
     3001 attcacggca caccagatac aactcaacta ggtatacctg gctcacatgg atgcattcgc
     3061 ctgaataatg atgcagttat tgagctggca gcttgggcaa caaagaatac tttagtatac
     3121 atacaatgat taataacaat taatgcttcg gagcataagt gaacctgaag gggtaaatga
     3181 atgtcctata aatttgaaga aggtaaagac gcaatagtaa aaagcgttgt tgaaaaaatt
     3241 aaacaaacaa tgactggcga acaaacagag ttttgcgctg aattcgcgaa gcaattttat
     3301 gggactgtcg cccttgaaga tcttctagag tgggaggttg atgatcttta tggagcagca
     3361 gtcaatttct ggtctttgat ttgtgaacgc gctccacatg aaacaaaaat caggatttat
     3421 aatcctgatt atgaacgtca tggatggcaa accacacata ctgttgtaga agttatctgt
     3481 gaagatatgc cttttattgt agattctctg aggatagtaa ttaatcgaat gggattaaca
     3541 tctcatctta ctattcatat gggaggaatt agggttaaaa gagacaacca taacagggtt
     3601 accgaaattc ttccaagaaa tggtggatct gctggagatg atattttaca tgaggcgccc
     3661 atttttatag aaattgatcg acaaacagac ccggctagct tatcggagct gcacaaaaat
     3721 tttgaacgcg ctcttgagga caatcgagca gtattcgaag attgggataa aatgcgtact
     3781 aaagtacgag aaattattaa ggagcttgat actgtaccaa agacaattga tatcagcgaa
     3841 atagaagaga cgaaagcctt tttaaattgg atggaagatc accattttac ttttttagga
     3901 ttgcgagatt atgatttggt taaaaaagga aaagaaacca ttttacaacc tattcctgaa
     3961 acaggattgg gagttttacg ccaaagtctg agcaaatcaa atgcaagaag tattacggca
     4021 atgggaccag aggcacaagg attaatttca tcaccccgca ttcttgtcat gtccaaaaca
     4081 aataccttag cctctgttca tagagatgct tataccgact atattggtgt taagcgcttc
     4141 aataaaaaag gagaggtgat tggcgaaagg cgcattatag gactgtatac ttctgcagct
     4201 tatcatacaa atcccaagca cattccgttt ttgcgacata aagtcgcatt aattatgaaa
     4261 aattctaatt taaatcccta tagccatgca ggtaaagtat tattgaatat acttgaaact
     4321 cttccaagag atgatttaat tcaaggcact gaggatgagt tactggaaat agctatgggt
     4381 attttctata tgcaagatag aaaacgtatc cgcctgtttg ccagagccga tgtttacaag
     4441 cgatttattt catgcttggt atatgttccc aaggatcggt ttaatactga attaagatat
     4501 gccatgcaaa aaattttggc agacagtttt aatgctgaag agattacctt ttctactcag
     4561 ttttcggaat cggtattggc aagaattcat tttatagtaa gggttaatcc caaaaattta
     4621 cccgattttg atttaaaaga aatagagcaa aaattgattg aagtggggcg gtcgtggatt
     4681 gatgatttgc aacatcattt acatgaagtc tatggcgaag aacaagccaa tgcactttac
     4741 tcccggtata aaaatgcgtt tccaatatct tattctgata ccttttcatc aagaacggct
     4801 gtctatgata ttaagcatat tgaaatgcta agccctgaaa atccactcgg tattaatttc
     4861 tataagccat tggatgaatc tgagaaaagc tttaggttaa aggtttatca gcatgacacc
     4921 actattcctt tgtctgatgt actacctatc ctggaaaaac ttggtttaag agcaattagt
     4981 gaacgtcctt atgttttaaa atttgaagat ggtaaagtag catggattaa tgattttgcc
     5041 atgcaataca acaaagaaag cgagtttaat atcgacgaaa taaaagagtt atttcaaaat
     5101 gcatttgcca gggtatggtt tggtgatgca gaaaatgatg gctttaattt attggtatta
     5161 gcggccggat taaattggag ggaagttgct gtattaagaa cctatgcaaa atatttcagg
     5221 caaatagggt ttacatttag ccaggattat atggaaacag cactaaataa taatgtaggc
     5281 attgccaaga aattagttag gttatttgaa attcggtgca atccccatga ctccaaaagg
     5341 agagaggata gatacgttgc acttgtcgct gaaattttat ctgatcttga taatgtagct
     5401 aatctggatg aagacagaat tataagacaa tacgtgcatg ccatcggtgc cactcttcgt
     5461 accaattttt atcaagtaga cgctcaaggt aatcctaaga attatatttc tataaaattg
     5521 tccagcaaac tcattcctgg tgtaccaaaa ccatacccca tgtttgaaat ttttgtttac
     5581 tcgccacgat ttgaaggggt gcatttgcgc tgtggtaaag tggctcgcgg aggtttacgc
     5641 tggtcggaca gacgtgaaga tttccgaacc gaaattcttg gcttaatgaa ggctcaacag
     5701 gtaaagaact ctgtaattgt ccccagtggc gcgaaaggtg gttttgtacc caaacagata
     5761 ccggctaatg ctactcgtga agaaattatg gaggagggaa tcagctgtta taaactcttt
     5821 ataagaggtt tgctggatat aacagacaat tacaaagaag gactcattgt aaaaccccaa
     5881 aatgttgttt tttatgatga agatgaccct tatcttgtgg tcgctgcaga caaaggcact
     5941 gcgacatttt ctgatattgc caattcaatt tcccaggaat atgatttttg gcttggagat
     6001 gcttttgcct caggtggctc tgttggttat gatcataaaa aaatggggat tactgctaaa
     6061 ggggcatggg aatctgttaa acgccatttc tatgaattga acagagatat ccaaaacaat
     6121 gatttcactg tggttggcat aggagatatg gccggggatg tctttggaaa tggcatgttg
     6181 ttgtccaggc atatcaaact cgtaggtgct tttaatcata ttcatatatt tgtagaccct
     6241 aatcctgaag ctgaagctag ctttaaagag cgtgagcgat tattcaatct tcccagatct
     6301 aactggacag actatgataa aaaactcatt tccaaaggag gaggggtttt ttctcgtagc
     6361 gcaaaatcta tcccggttag ccctgaaatg cagaaagtat ttggaataaa acaaacttcg
     6421 attgaaccta atgatctaat taaagcaata ttaaaagccg atgtggattt actttggagt
     6481 gctggaattg gaacctatgt caaatcgagt actgaaagta ataccagcgt aggagatcga
     6541 acaaacgatg caactcgtgt taatgccaat cagttgagat gcaaagttat aggcgaaggt
     6601 gggaatttgg gattaaccca gctagcacga gtggagtata gtttacacgg aggcaaagtc
     6661 tatacagatt tcatagataa ttctggtggg gttaattgct cagacaaaga agttaatatt
     6721 aagattttat tgaataatgt tgtatcagct ggggatctga ctccaaaaca aagaaatgag
     6781 cttttaagta atatgactga tgaagtagcc aaattagtgc ttagagataa tttcctacaa
     6841 acccgagcca ttagcctaac tgcttctcaa gccttaagag ccattgaatt acatgcaaga
     6901 tatattaacg aactagaaaa aacaggaaaa attgacagaa ctttagagtt tttacctgat
     6961 gaaaaagtgc taatggaaca caaactaatg gggaaaggat taactcaacc tggaattgct
     7021 gttttaatgt gctacagcaa aaccatatta aaagagcaag ttcttgcttc tgaagttcct
     7081 gaagaagatt atatgaacca aatacttatt ggttcatttc ccaaacctct gcaagagcgt
     7141 tttagtaaac aaatgcaaga tcatccctta agaagagaga taattgctac ccgtttaagt
     7201 aacattattg ttaatgaaat gggtttcacg tatgtttaca ggttacaaga tgaaactggg
     7261 gcatctgttg cggcaattgt cagggcctat atgattgcca gaagtgtctt gaatctggaa
     7321 gcaatatgga agcaaattga ggaacttggt acaaaaatca gcgcgcaagc tcaagttgat
     7381 atgatgatgc tgtatgttcg actatccagg agagtgacaa ggtggttttt acggacacat
     7441 agaaggtcga tgagtataac gcaggcgata gagttatacg caaaaggggt tgatgaactg
     7501 aaaaaatcca tgcctgccgt ttttggcgaa acaggccgga ttcaatatga ggagcattac
     7561 caagagcgga taaaagaagg gataccgcct caacttgccc atgaattaac agtgactcga
     7621 ggcctgtttg ccgcaacaga cattatagaa attgcctacg aggaaaatat aaaaatacca
     7681 aaagtagctg aaatatattt tggtattgga gaatttcttg acatagcttg gcttagaacc
     7741 caaattattg ttcacacaac tgaaaatcat tgggaatctt tatctcgaga ggcattacgc
     7801 gacgatttgg attggcaaca acgccagtta acagctgcca taatgggttt tgagcctaat
     7861 aataaggatt tgcaagaacg attaacaaga tggggagaga cacacgtttc tttaattgac
     7921 aggtggaatt atattctgac agctctgaaa tcaagtacag ctttgaacta tactatgttt
     7981 ttagttgcca cccgagagtt gctggattta acccaaacga cattacaatc caatttaaaa
     8041 atggaagagt cattttgaca tgaaaggagt taaatgaaat ggaggctaaa actgcttcag
     8101 aatattcaac actgagcaaa ttatttcatt ggattattgc attggcagtt ataatcatgc
     8161 taatagcagg tttttttctt gatgaaatac ctgaggaatt tcagggggtt gcttatatgc
     8221 ttcataaatc aacaggaatt actatcctgt tcttgatgat tttgcggttt atttggatcc
     8281 atgctaaagg aaaacctgct ttacctgcct ctgttaaatc ttgggagaaa atattatcaa
     8341 ggtttgtgca atatggtttt tatgtgctct tgattgttat gccattgagt ggttggatca
     8401 tgtcagttgc agcagacaga ataccaaatt actttggctt gtttaaagcg ccacttccat
     8461 ggattgaacc taataaatct ctagcaaaat taatggctga atcccatgaa attattgcgt
     8521 gggttttaat cgccttcatc acattacatg ttttgggtgc tcttaagcat cattttattg
     8581 ataaagacaa tgtattgaag cgtatgctgc ctggaaaaag caaatagcct tctgtttttg
     8641 aaaattgagc ctgaacttca aagttcaagc tcaatttttt cttctaattg gattggaaat
     8701 ttattgcctt ccaaacagac agatgaaaag ttctctttta atatgcttgc agcagctaaa
     8761 tatcgatttt caccaagcat ggagtaatca attaactctc caatctctgt gtcttcatcc
     8821 gatttaaata atttttgacc agaatataat tgattatctg actgaattac aaatttcttt
     8881 aattcatgct taagagtagc cctgtagtga gttcttgcga tgatttcctg acctttataa
     8941 caccctttat caaaactgac gtacgcggtt tgatgtaagc caattctatg tggtaaaaag
     9001 agtcccctag aattaggata aatatcaatt tgattgttaa ataatctaag ggtatgccag
     9061 gttaatgaac caagtaattg attattttca ataaaaggct tgcacaaact atcataatag
     9121 tctgaatgga ttaaatagat atagaatcct ttgcctaagt gataaacaca accatgactc
     9181 gtacaagata aagcatacaa ttcgctgggc aaaggtagta actgtggtat ttgatcatta
     9241 ccatgctgta aataaaagcc taaaatttta tagttgtgat ttgaagtgat tttaactcga
     9301 gaaagcagtg caactttgtt taatgaattc tgagttactt caattaaatc ttggggcaga
     9361 actaatttta caccttgcca gttgataata tccaataagg aaagaattct ccctttaagg
     9421 ttacattggg ccccttgaat cattgaagta tccgatacca atcgtaaatc acaagttaac
     9481 tgcccttgca gaaaatctat tgatttctct ccaaacaaat cga
//
LOCUS       DASLOE010000117         9366 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.118, whole genome shotgun sequence.
ACCESSION   DASLOE010000117 DASLOE010000000
VERSION     DASLOE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9366
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.118"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            87..1049
                     /locus_tag="U6309_002147"
     CDS             87..1049
                     /locus_tag="U6309_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEN5505585.1"
                     /translation="MNFCTLGLAVIETEAQAVFELTQRIDEHFEKACELLLSCKGRIV
                     VTGMGKSGHIANKLASTFSSTGSPAFFMHPGEASHGDLGMITRQDIVVAISNSGNTHE
                     IVTLLPLLKRLEVPLITLTGNKQSTLAKSADVNLDVSIKQEACPLGLAPTTSTTVSLV
                     MGDALAISLLQARGFSAEDFALSHPGGALGKKLLLKIDDLCHTGEQLPLANENATVSD
                     ALIEVTNKKLGMTCVVDNHGYLVGVYTDGDIRRTLTRQFNINTTLIKDVMTKNCRTIS
                     KGMLAAEALAIMQKYSITSLVVVENDNRPYAVLHLHDLLKAGVF"
     gene            1063..1590
                     /gene="kdsC"
                     /locus_tag="U6309_002148"
     CDS             1063..1590
                     /gene="kdsC"
                     /locus_tag="U6309_002148"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HEN5505586.1"
                     /translation="MNELLEKAKKIKCLICDVDGVLSDGLLHIDNHGNELKSFHVQDG
                     MGLKLLMAAGIQVAIITTAQNAVVDHRMEQLGITHYYKGQVDKRSAYQHLKKTLGLND
                     EEFAYIGDDLPDLPLIQQVGLGVAVSNAVPQVLEFADWRTERTGGRGAVRELCDLILN
                     AQNKAELAITGYLKQ"
     gene            1587..2156
                     /gene="lptC"
                     /locus_tag="U6309_002149"
     CDS             1587..2156
                     /gene="lptC"
                     /locus_tag="U6309_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEN5505587.1"
                     /translation="MNITKQAIWLFLTLLILAGSGWYYAHFNQAIINKKTLSNEVDTT
                     ISQLTVKQFNSEGVLINLLTTPQVEYIPNNNLHLLKNPHIIIKQDNQPAWEINSLRAK
                     SFDGGKQITFIEQVVVHQNPGENTRESTLKTEEITYYPKEKKATTSLFVTFEQPGNII
                     QSTGMNAYLDEKRVELLHKARGSYDPAKG"
     gene            2137..2646
                     /gene="lptA"
                     /locus_tag="U6309_002150"
     CDS             2137..2646
                     /gene="lptA"
                     /locus_tag="U6309_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEN5505588.1"
                     /translation="MTQLKVKYIILLLLMLCSISGNALPFDKEKMVQVVSDSADLNQK
                     NHQGVYIGNVEFVQGSTNLRAEKAITQGDAKNQLTLAIAEGSKDKQAHYWTTTAPDKP
                     PFHAYADTIKYYPLKHLIELIGNARIEQGPNSLSAAKISYDTQEQHVLSHSDEKTRTT
                     IIIYPEKKS"
     gene            2643..3368
                     /gene="lptB"
                     /locus_tag="U6309_002151"
     CDS             2643..3368
                     /gene="lptB"
                     /locus_tag="U6309_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEN5505589.1"
                     /translation="MSLLEAKQLKKSFKGRTVVNGVSIHIKQGECVGLLGPNGAGKTT
                     CFYMIVGLQSCDEGHIILNGTDITQDAMHLRARHGISYLPQEASVFRKLTVAENIMAI
                     LELRKDLDDQKREEKLDLLLQEFHISHIENSLGMALSGGERRRVEIARALAIEPSFIL
                     LDEPFAGVDPISVIDIKKIILHLCDKGIGVLITDHNVRETLDICERAYIVSQGKILCE
                     GTPKAILANQQVRQVYLGEEFAL"
     gene            3402..3980
                     /locus_tag="U6309_002152"
     CDS             3402..3980
                     /locus_tag="U6309_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEN5505590.1"
                     /translation="MLLIIDNYDSFTYNLVQYFQSLNQKVAVFKNDEIQIDAIRQSAP
                     QYLVISPGPNSPDESGISIEVIKEFHKHIPILGICLGHQCIAQAFGGKIIPAPEIMHG
                     KTSEITHHYQGLFTGIPNHFQATRYHSLAVDIMSLPNCFSIDAWADDTIMAISHRQFP
                     LFGLQFHPEAILSQFGLQILENFLRYETQTSI"
     gene            3958..4992
                     /gene="trpD"
                     /locus_tag="U6309_002153"
     CDS             3958..4992
                     /gene="trpD"
                     /locus_tag="U6309_002153"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEN5505591.1"
                     /translation="MKPKLLFEQLLSRQDLSSDQMQEVIHACMTGEFSDVQIATFLAL
                     MRMKGETVNELTAAAKVMRQLAHKIDLGNPLIDIVGTGGDGRNTFNVSTACSFVVAAA
                     GIKVAKHGNRSVSSRSGSADLLEQAGFILNLSDSQVQNCINQCQLAFLFAPHYHPAMQ
                     HARAARQQLGIRTLFNLLGPLINPAQVKRQVVGVFSTNWLKTIATVLANLGSERSLVI
                     SSQDGLDEISIAAKSEVVEYRDGNFKQWFISPEDYGLKHSSLDAIIVDSPEQSLHLIQ
                     SVLSGDSGPARDIVLLNSAAAIYCAKDGISFDAAIEEAKIAIDSGKANHCFNKLRLLT
                     QTLNKESNHE"
     gene            4985..5761
                     /gene="trpC"
                     /locus_tag="U6309_002154"
     CDS             4985..5761
                     /gene="trpC"
                     /locus_tag="U6309_002154"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEN5505592.1"
                     /translation="MNSILERIAKHKLEEVAVAKKNKPLHVLSKQQPGEIRDFITALK
                     SNASPAVIAEIKKASPSKGLIRKDFNVAEIAKIYTQNGARCLSVLTDIEFFQGHPDYL
                     ALAKSKTTLPVLRKDFIIDSYQIYESLVLGADCILLIVALLDDVQLMDFCQLAQELKM
                     SVLVESHTQDELERALRLPTPLIGINNRSLHNFKTDIQLSIQLKQFVPKDKIIITESG
                     INTREDIKLMQSHGINAFLIGESLMRADNIGKALQKLMTD"
     gene            complement(5767..5955)
                     /locus_tag="U6309_002155"
     CDS             complement(5767..5955)
                     /locus_tag="U6309_002155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505593.1"
                     /translation="MNTTEIPEQPIEYPEQPIEYPEEPSEYPEQPLEYPDNPEPLEPG
                     YPELPEPEPSQTPEPGFE"
     gene            6258..7106
                     /locus_tag="U6309_002156"
     CDS             6258..7106
                     /locus_tag="U6309_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN5505594.1"
                     /translation="MNVVIITGAASGIGHALSQVCLSNGKTIVMVDKNQDKLHTEAQS
                     FMTKFSPENVICYACDVTKPSEVERLAEYSCKQLGQVDWIFNNAGIIGNLLPAWELQE
                     NDINQVMQVNLHGMLNVIRSFMPYLFKQNFRSHIINMASLYALCTGSQMAAYSMSKHA
                     VLALSESLYFDLNRLKKPVDVSVVFPSFTDTSLLASSGKLNHSPIHNQLNNLLAHSRP
                     AIEVAEHIVREVEQKRFYILPDKEVKGYCEDRTKAILLQENPHRNSVEQLMCSLLKRQ
                     SSEANL"
     gene            complement(7229..8539)
                     /locus_tag="U6309_002157"
     CDS             complement(7229..8539)
                     /locus_tag="U6309_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEN5505595.1"
                     /translation="MEIKRVLKDRLSARVCVIGAGPSGIAAIKNLQEHGITNVTVFEK
                     NNQIGGNWVYDEQNEHSSVYETTHIISSKRWSEFEDFPMPVDYPDYPSHSQLLKYFQS
                     YVEHFHLDRYIRFNTVVQKVHRLDDNTWHVIYEDAQGIHEACYDYLLVANGHHWDPFM
                     PVYPGVFDGQILHSHQYKKASIFKGKRVLVVGGGNSACDVAVEISRVAPGTCISMRRG
                     YHIFPKFIFGKPTDVAVAKIQWMPSWLRQKFISLVIRGLQGRYAKYKLMKPDCGPLEI
                     HPTINSELLYFIRHGKIHPRPGISRFEGNTVHFTDGTQGEFDTIIFATGYQISFPFFD
                     KSCIDFSNSTTIPLYRKMMHPEFENLYFIGLCQPQGCIWPLADYQSKIVARIIAGSLK
                     RPDQLHRKIAKEINKPHHRFKSHMRHALEVDYHIFRKELLEMLG"
     gene            complement(8517..9242)
                     /locus_tag="U6309_002158"
     CDS             complement(8517..9242)
                     /locus_tag="U6309_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505596.1"
                     /translation="MNYFGNLQIRFSKSFLLLLIVSSLTACVDLTGARNTYIKKEKQP
                     AISDVPNKKSKAQNMSSKKGEVHTMLGGLGIFSKGMYTLRDKVSEKYHITAHSTMWYN
                     AGAVSRYIIDHYYQEKDHKPIILVGHSLGANEQIKVARNLNAAGVPVALLVTVDAVSQ
                     TIVPPNVKQALNVYKPGFVPMFSGLKLRAVDPQLTKIDNINVDSIKGIKVNHFTIDKD
                     EILQAMIMDKVDKVLVDGNKKGA"
BASE COUNT         3025 a         1805 c         1786 g         2750 t
ORIGIN      
        1 cggtatcact tactgaaata tagggtacat catttaaata cattatgtta gaatgaaaac
       61 caaacaaaat tacgactcaa tgacctatga acttttgcac acttggacta gcagttattg
      121 aaactgaagc acaagctgta tttgagctaa ctcaacgaat tgatgaacat tttgaaaaag
      181 cttgtgaatt actcttatca tgcaaagggc gtatcgtcgt cacaggcatg ggaaaatctg
      241 gccatatagc taataaactg gcctctactt tttccagcac tggcagcccc gcttttttta
      301 tgcatcctgg agaagcaagt catggagact tgggtatgat aactcgccag gatatagttg
      361 ttgccatttc caattcaggc aatacccacg aaatagttac tttgcttcca ttattaaaaa
      421 ggcttgaagt ccccttaatt acattgaccg gcaataaaca atcaactctg gctaaatctg
      481 cagatgtcaa tctggatgtc agtattaaac aagaggcttg ccctctgggt cttgctccaa
      541 caaccagtac tacggtatcg ctggttatgg gggatgcatt ggctatatct cttttacaag
      601 cacggggatt tagtgcagag gacttcgctc tttcccatcc tggaggcgca cttggcaaaa
      661 aattgttgct aaaaatagac gatttatgtc atacaggaga gcaacttccc ttagcgaatg
      721 aaaacgcaac agtgagtgac gctttaattg aagtcacaaa taaaaagctg ggaatgactt
      781 gtgttgtaga caaccatggc tatttagtgg gtgtatatac tgatggagat atccgtcgaa
      841 cattgacacg tcaatttaat atcaacacta ctctaatcaa ggacgtcatg actaaaaact
      901 gcagaactat ttctaaaggc atgttggctg ccgaagcttt agcaatcatg caaaaatata
      961 gcatcacatc cttggtagta gttgaaaatg ataatagacc ttatgcggta ctccatttac
     1021 atgacttgct aaaagcgggt gttttttaat acagagatag aaatgaacga actattggaa
     1081 aaagcgaaaa aaattaaatg tcttatttgt gatgtagacg gggtgctcag tgatggctta
     1141 cttcacattg ataatcacgg caatgagctg aaatcattcc atgtacaaga tggaatggga
     1201 ttaaaattat taatggccgc cgggatacaa gttgcaatca ttaccacagc acaaaatgct
     1261 gtagttgacc atcgaatgga gcaattaggg ataactcatt actataaagg tcaagttgat
     1321 aagcgtagcg cttaccaaca tctaaaaaaa acattgggac tcaatgatga ggagtttgcc
     1381 tatattggcg atgatttgcc agatttaccc ttgatacaac aagtgggatt aggagttgca
     1441 gtcagtaatg ctgtaccgca agttctggaa tttgctgatt ggagaacaga gcggactggt
     1501 ggtagaggcg cggttcgcga attatgtgac ttaattctta acgcacaaaa taaagcagaa
     1561 ttggcgatca caggctattt aaaacaatga atattaccaa gcaagcaatt tggctattct
     1621 tgaccttgct tattttagca ggatctggtt ggtattatgc tcattttaat caggctatta
     1681 tcaataagaa aacgttgtca aatgaagttg acacaacgat ttcgcagctt accgtaaagc
     1741 aatttaattc agaaggtgtg ttaattaact tattaaccac tccacaggta gaatatattc
     1801 ctaacaacaa tctccatttg cttaaaaacc cccatattat tattaagcaa gataatcaac
     1861 ccgcgtggga aatcaattca ttacgggcaa aatcgtttga tggtggtaaa caaattactt
     1921 ttatagagca agtagtagtt catcaaaatc cgggagaaaa caccagggaa agcactttaa
     1981 aaactgaaga gattacttat tatcccaagg aaaaaaaagc aacaacaagt ctttttgtaa
     2041 cctttgaaca accaggcaat atcatacagt ctactggaat gaatgcctat ttggatgaaa
     2101 aaagagtaga attactccat aaagccagag gtagttatga cccagctaaa ggttaaatac
     2161 attatactgc tattgttgat gttgtgctcc atttcaggaa atgctcttcc gtttgataag
     2221 gaaaaaatgg tgcaagtagt ttctgattca gccgacttaa atcagaaaaa tcatcaggga
     2281 gtctatatag gaaatgttga gttcgttcaa gggtcaacca atttgcgtgc agaaaaagcc
     2341 attacgcaag gagatgccaa aaatcaactc actttagcca ttgctgaagg ctctaaagac
     2401 aagcaagctc attattggac tacaacagcc cctgataaac ccccattcca tgcctatgct
     2461 gacactataa aatattatcc gctaaaacat ttaatcgaat taattgggaa tgctcgcata
     2521 gagcaaggac ccaactctct ttcagcggca aaaattagtt atgatactca ggaacaacat
     2581 gttttatcac atagtgacga aaaaacaaga acgacaatca ttatttatcc tgaaaagaaa
     2641 tcatgagttt actagaggct aaacaattaa aaaaatcatt taagggccgg acggttgtga
     2701 atggtgttag cattcacatt aagcaaggtg aatgtgtcgg tttattaggt cctaacgggg
     2761 cgggtaaaac cacttgtttt tatatgattg taggtctgca atcctgtgat gaaggccata
     2821 ttattcttaa cggaacagac ataactcaag acgcaatgca cctaagagca aggcatggta
     2881 ttagttacct tccgcaagaa gcctccgttt ttagaaagct tactgtcgct gaaaatatca
     2941 tggcgattct ggaactaaga aaagatttag atgatcaaaa acgtgaagaa aagctggatc
     3001 ttctcttaca agagtttcat atttctcaca tagaaaacag cttgggcatg gccttatcgg
     3061 gcggcgaaag aagacgcgtt gagattgcaa gagccctggc aatagaacca tcctttatct
     3121 tgcttgatga gccttttgcc ggggttgatc caatttctgt aatcgatatc aaaaaaatta
     3181 tcctgcattt gtgtgataaa ggtatagggg ttttaattac agatcataac gtgagagaga
     3241 ctttagacat ttgcgaacgc gcttatattg taagtcaggg aaaaattctt tgcgaaggaa
     3301 cccccaaagc aatacttgcc aatcaacaag taaggcaagt ttatttgggt gaagaatttg
     3361 ctttgtagcc tcatttaaaa gaatacattt taattatact tatgttacta attattgata
     3421 actacgattc atttacatac aacctggtac agtacttcca gagcttaaat caaaaagttg
     3481 cagtttttaa aaatgacgaa attcaaatcg atgctatcag gcaatcagcg ccgcaatacc
     3541 tggtcatttc accgggaccc aactctcctg atgaatcagg aatctctata gaagtcatta
     3601 aagagtttca caagcatatt ccgatattgg gtatttgcct tggccatcag tgtatagctc
     3661 aggcatttgg aggcaaaatc atcccagcac cagaaatcat gcatggtaaa acctcagaaa
     3721 tcacccatca ttaccagggc ttatttacag gaataccaaa tcactttcaa gcgacgagat
     3781 atcattcttt agccgtggat atcatgagct tgcccaattg cttttcgata gatgcgtggg
     3841 cagacgatac aataatggca atatcacatc gtcaatttcc tctttttggt ctacaatttc
     3901 atcctgaagc catattaagt caatttggct tgcaaatact cgagaatttt ttacgctatg
     3961 aaacccaaac ttctatttga acaacttcta tcccgacagg atttaagctc agaccagatg
     4021 caagaggtca tccatgcttg tatgacaggt gaatttagtg atgtgcaaat tgcaaccttt
     4081 ctggcattaa tgcgcatgaa aggtgaaaca gtcaatgaac ttactgcagc agctaaagtc
     4141 atgaggcaat tagcccacaa aatagatttg ggaaatcccc tgattgatat agttggcact
     4201 ggcggtgatg gaagaaatac ttttaacgta tcaacagcct gtagttttgt tgtagctgca
     4261 gcaggaataa aagttgcaaa gcatggtaac cgttctgttt cgagtcgtag cggtagcgct
     4321 gatctattag aacaagccgg atttatactt aatctttctg actcacaggt acaaaactgc
     4381 atcaaccaat gtcaattggc tttcttgttt gcgccacatt atcaccccgc catgcagcat
     4441 gcgagagctg caaggcaaca attaggaata agaactttat ttaatttact tggcccactg
     4501 attaacccgg ctcaggtaaa acgacaagtc gttggtgtat tttcaacgaa ttggctcaaa
     4561 acaatagcta ctgtcctggc caacttaggt agtgaaagat cattagtaat aagctctcaa
     4621 gatggactgg atgagattag tattgcagcg aaaagcgaag tagttgaata ccgagatgga
     4681 aattttaagc aatggttcat ttctcctgaa gattatggtc taaagcattc ttctttagat
     4741 gcaatcatag tcgactcacc agagcaaagc ctgcatttga ttcaatccgt actttcaggt
     4801 gattctggtc cagctcgaga tatagtcctt ctcaattcag ccgcagccat ttattgtgcc
     4861 aaagatggca tatcgtttga tgcggccata gaggaagcaa aaattgccat tgatagtggt
     4921 aaggcaaatc attgttttaa caaactccga ttattgactc aaactttaaa taaagaatca
     4981 aatcatgaat agtatcctgg aacgcatagc caaacataaa ctagaagaag ttgcagttgc
     5041 gaaaaaaaat aagcctttac atgttctgtc aaaacaacaa ccaggtgaaa tacgcgactt
     5101 cataactgct ttaaaatcca atgcctcacc tgcagtcata gctgagataa aaaaggcttc
     5161 tccaagcaaa ggtttaattc gaaaagattt taatgttgca gaaattgcca agatttatac
     5221 ccaaaatggt gcaagatgct tatctgtttt aacggatatt gagttttttc aaggtcatcc
     5281 tgattatttg gctttagcaa aatcaaaaac tactttgccc gtattgcgta aagactttat
     5341 tattgattct tatcaaatct atgaaagcct tgtcttgggc gctgattgca ttttactgat
     5401 tgttgcatta ttagatgatg ttcaattaat ggatttttgc caactggctc aagaacttaa
     5461 aatgtctgtt ttggttgaaa gccacacgca agatgaatta gaaagagcat tacgcttacc
     5521 cacccctctt ataggcatta ataaccgcag cctacacaac tttaaaacag acattcaact
     5581 gagtatccaa ttaaaacaat ttgttcctaa agataaaatt attattactg aaagcggaat
     5641 caacacccgt gaagacataa aattaatgca atcccatggc attaatgcct ttttgattgg
     5701 agagagttta atgagggcag ataacatcgg gaaagcatta cagaaactaa tgactgacta
     5761 aataaactac tcaaaacctg gctcaggagt ttgacttggc tcaggctcag gtaattcggg
     5821 ataaccaggc tccagaggct ctggattatc agggtactcc aaaggttgtt cagggtactc
     5881 tgatggctcc tctggatact caattggctg ttctggatac tcaattggct gttctggtat
     5941 ttcagttgta ttcaaagtat cgcctccttc tgatagttct attcaaagta tagcaaaatc
     6001 aattttagca atcattttgg taaatactct tgcccatgat acactttttc ataaaatcca
     6061 cattagaaac ctcaatgcct ccctagcgcc acagaaacag gacttacgaa aaaccccaag
     6121 gccaaggcaa aaaaagtttt taatgaggga gtttagataa actaaatgac cgaattaaaa
     6181 acttttttta acgaagagat tggggttttt cgtaagtcct gaagaagagg gcattaaact
     6241 tttgtaaggt attttatatg aatgtagtaa ttatcactgg tgcagcaagt ggcattggac
     6301 atgccttaag tcaagtttgt cttagcaatg gaaaaactat agtcatggtt gataagaatc
     6361 aagacaaatt acatacagaa gcccaatcat tcatgactaa attttctcct gaaaatgtta
     6421 tttgttatgc ctgcgatgta accaaaccat cagaagtaga acgacttgcg gaatattcct
     6481 gtaaacaact aggccaggta gactggatat ttaataatgc tgggatcatt ggtaatttac
     6541 tccctgcctg ggaactgcaa gaaaatgata ttaatcaggt aatgcaagtg aatttgcacg
     6601 ggatgcttaa tgtgattcgc tcattcatgc cttatttatt caaacagaat ttccgttctc
     6661 atattatcaa catggcaagc ctgtatgcct tatgtacagg ctcacaaatg gccgcttatt
     6721 ccatgtcaaa acatgctgta ttagccttat ccgagtcatt atattttgat ttaaatcgtc
     6781 ttaaaaaacc tgtagatgtt tctgtagtat ttccttcttt tacagatacc tcattattag
     6841 cgagtagtgg gaaattaaac cattctccaa tccacaatca attaaataac ttgttagcac
     6901 attctcgacc cgccattgaa gttgcagaac atatagtaag ggaagttgaa caaaaaagat
     6961 tttacatact gcctgataaa gaagttaaag gatattgtga agacagaacc aaagccatac
     7021 tgttgcagga aaatccgcat cgtaacagcg tcgaacaatt aatgtgttct ttattaaagc
     7081 gccaatcatc agaagccaat ctgtaaattg tttgcgattt tcaaaacaga ataattgtta
     7141 cccggtttat aactgatgtg atcatccatg ctttaacatt catgtactca ttacgaatct
     7201 aggggtagaa tttcagtctc ctatgttttt agcctagcat ttctaacaat tcttttctga
     7261 aaatatgata atcgacctct agagcatggc gcatgtgaga tttaaatcgg tgatggggtt
     7321 tattgatttc tttggctatt tttcgatgta attgatcagg tcgtttcaaa gaccctgcaa
     7381 taatgcgagc cactattttt gattgataat cagccaaagg ccaaatgcaa ccttgaggct
     7441 ggcataggcc aatgaaatac aaattttcaa actcggggtg catcattttt cgatataaag
     7501 ggatggtggt agagttacta aaatcaatac aacttttatc aaaaaatgga aaactgattt
     7561 ggtaacccgt agcgaaaatg atcgtatcga actccccctg ggttccatca gtaaaatgaa
     7621 cggtgttacc ctcaaatctc gatataccgg gacgagggtg tattttgcca tggcgaataa
     7681 aatacaagag ttcggaatta atagtagggt gaatttccaa tggcccacaa tcaggcttca
     7741 ttaatttata cttggcgtac cggccttgca atcctctaat gaccaggctg atgaacttct
     7801 gtcttagcca ggatggcatc cattgtattt tagctaccgc tacatccgtt ggcttaccaa
     7861 aaataaattt ggggaaaata tgataacccc tacgcatgct aatgcaagtt ccaggcgcaa
     7921 ctcgcgatat ttcaacagcg acatcacagg ctgaattacc cccgcccaca accaatactc
     7981 gcttgccttt aaaaattgaa gcttttttat attggtgaga atgaagtatc tgcccgtcaa
     8041 atactccagg atatacaggc ataaagggat cccaatgatg accgtttgct accagcaaat
     8101 agtcatagca cgcttcatga attccttggg catcctcata gataacatgc catgtattat
     8161 catcaagtcg atgtaccttt tgtaccactg tgttaaaacg aatgtatcta tctaaatgaa
     8221 aatgctccac ataactctga aaatatttta aaagctggga gtgtgaagga taatccgggt
     8281 aatcaacagg catagggaag tcttcaaact cagaccagcg ttttgaacta atgatatgag
     8341 tcgtctcata aacgctggaa tgttcatttt gctcatcata aacccaatta ccacctattt
     8401 gattgttttt ttcaaaaaca gtaacattag taataccatg ttcttgcaaa tttttaatgg
     8461 cggcaatacc gctgggacca gcaccgataa cacaaacacg agcagataga cggtccttaa
     8521 gcaccctttt tatttccatc aactaacacc ttatcaactt tatccattat catggcttgt
     8581 aaaatttcgt ctttgtcaat tgtaaaatga tttactttta tcccttttat cgaatcaaca
     8641 ttgatattat caatctttgt caattgagga tcgactgctc tgagttttaa cccgctaaac
     8701 atcggtacaa aaccaggctt atatacattc aaagcttgtt taacattagg aggcacaata
     8761 gtttgtgata ctgcatcgac agtcaccaac aaagcaacag ggacacctgc ggcattcaaa
     8821 tttcgagcca ctttgatttg ttcattagcc cctaaagaat gccccactaa aataattggc
     8881 ttgtgatctt tctcttgata gtaatggtca ataatatacc gacttaccgc tccagcgtta
     8941 taccacatgg tgctatgagc cgtgatatgg tatttttcag acactttatc acgaagtgtg
     9001 tacatcccct tgctgaaaat tcccaggccg ccaagcatag tatggacttc gccttttttt
     9061 gatgacatat tttgtgcttt ggactttttg ttagggacat cagaaattgc aggttgcttt
     9121 tctttcttga tataagtatt tcgtgcgccc gtcaaatcca cacaagctgt taaactacta
     9181 acaatcaaca acaataaaaa tgatttggaa aaacgtattt gcagattgcc aaaatagttc
     9241 atattaatat tattattgta attatttgat tttttatcat accttattct tgcaccattt
     9301 tacagaggca cctggcgggt ttaaggctat tcacgaattc attttaatgc cttgagaata
     9361 atctgt
//
LOCUS       DASLOE010000118         9288 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.119, whole genome shotgun sequence.
ACCESSION   DASLOE010000118 DASLOE010000000
VERSION     DASLOE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.119"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            176..895
                     /locus_tag="U6309_002159"
     CDS             176..895
                     /locus_tag="U6309_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505597.1"
                     /translation="MTERKKTTVKNKKAPVKKSQLTVSLLIFIDLISILLSLMPGVAA
                     FSLTGLFLYYGFSKTWLVLICSPFVLLFSFILIIALIRISLPKLKPGRYKRELNKTMI
                     AWFCHFALSRAAKIVGLIHLLQSFNVIKYLYWRALGVKVSFRVMNSFDIDFVDCPLIS
                     IGKDVTLASQVSISCHSDVGNYLFLSPVVIEDNVFVGMTTKIGPGTTIKKGAWIGYGN
                     AFVNQIIEENAKIGNVRPVPE"
     gene            1038..2537
                     /locus_tag="U6309_002160"
     CDS             1038..2537
                     /locus_tag="U6309_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505598.1"
                     /translation="MKKIVYVLLFISSLTHANCVIYFNEQPVNKRLSDPLYSLLSNTE
                     ICPQSIQELRNSMRLRGLKEVIGMVANRGRNNPGEGSFSFFESVYGRLANDQLIAKGE
                     LFLGYFTRLKDGLVILDQKPRKGKLLIEAISWDAKKALYNFYELRGIEHGGVRWFYRG
                     NSKDAYKDNTWLYRISPEGENHFGSRMRCSACHNSGGPILKEQSLPHNDWWTNQRPLI
                     LLPNKADGDVSQLLEQVSDASVFANEVITGMRHLSDSKKMQAFQGKLSLQEQLRPLFC
                     TTEINLQSSSDSSETVVSIPSAFWLNPLLGDINLTISAEDYNQLLKAFGTRFPETSLQ
                     DADHAWLTPVKAEADLNAIKQLIQQNIISKKFAQSVLMIDFTNPLFSSKRCELLKLIP
                     ESYQTNSKEWVEQFLINLRNNEEQFKGARQLADYMSKEQLDSKELMKKIKIYEYFLNQ
                     SISTSTGLRRHFQHLIELRKAVFRSELSQNPLGQILEPGFRVIFPEIQQ"
     gene            complement(2603..3724)
                     /locus_tag="U6309_002161"
     CDS             complement(2603..3724)
                     /locus_tag="U6309_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505599.1"
                     /translation="MAAQRHLYIFTLIGLLLGVAVDILIRYNNTTAFIYSVVTIFGVL
                     FALTYNNVNFSRLIGTSFLLAFFLSIPLFPLKMDYSMKDYFHFFTFFVGFPFFIYVAH
                     CFHYAYHHDNTWRVSYSSLFAGVWNTIPLLFIALVFSSLANLLIVLGSFVFKTVGNNY
                     LWDLYFYNRDFKLISNITLFFMGLGVGQQNLNIIHNMRFLLLRIMYYLFPLLAAISIL
                     YFILYTFHSFSSSQEHINPLIVLIPLTTAGIIFFNAYFQDGTIKSDYPSWLKLSLRVY
                     RVILFLLALMMTYKILSDFSLDTNAFIYLLVAVLFSFTYAITAFLNENQEKQWIYMGN
                     IGTAIFFIVTLFLCNLPYIPVEFTIGGGNAINFITSTLS"
     gene            complement(3886..4971)
                     /locus_tag="U6309_002162"
     CDS             complement(3886..4971)
                     /locus_tag="U6309_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEN5505600.1"
                     /translation="MKNLYIDPDITKASTISSEFYTSTEWFEITKEKIFSKTWQFCIS
                     TEELTTNRQLIPFTLLPGFLEEPLLFTKDESGTIRCLSNVCTHRGKILIEEPCVAHKI
                     KCSYHGRRFNFCGELIHMPEFELTKDFPSSNDNLSQISTGILEPFLFASLSPIVPFHE
                     VFAEIKERLFWLPMEKMRLDKEKCKDYVVKSHWALYCENYLEALHIPFVHPSLRKVID
                     CNTYTTELFRYCNLQLALASPDEVSFDLPKGAQDYGKQVAAYYFWIFPNTMLNFYPWG
                     CSVNVVKPLSPELTQVSFLSFVLDESKLGKGAGGALDQVEQEDERVVESVQRGIKSRY
                     YDMGRFSPTKEQGTHHFQRLLCEFLND"
     gene            5179..6153
                     /locus_tag="U6309_002163"
     CDS             5179..6153
                     /locus_tag="U6309_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HEN5505601.1"
                     /translation="MRSSRFLCLLNGMLLSSNVAAFSPSAPIFSPYVDLTINTHWDSQ
                     SQDMEPMDLISPAKKLGIKAYHLAFITDSGQCQPAWGAQQNYSVAKGWGKKQFDVLSR
                     EGLKLTVSFGGASGTDISYHCDKNQLINTFNQVVNQYHANVLDFDIENGTANIPNLLQ
                     ALKLFQKEHPDVLLSFTLPVMPEGLTSVGKEIITSAANLGLHFNVNIMAMDYGPAYSG
                     DMGDYAISAATNLHQFLQEIYPDKKPEALWQMIEVTPMIGVNDVNTEQFTLSNAAQLK
                     QFAQKNLLGGLSMWSFNRDKPCADKWASPVCSGNNLQSHDYEFVKYFQ"
     gene            6403..8175
                     /gene="lem20"
                     /locus_tag="U6309_002164"
     CDS             6403..8175
                     /gene="lem20"
                     /locus_tag="U6309_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem20"
                     /protein_id="HEN5505602.1"
                     /translation="MKSKEERFNEWRQEKAKSEQAYIKTVPLVEEYIKSFDTLTELRN
                     DVANPDSEKIKKMDAHKKRTLGVLLYAKLKLDEVIEKIPDSDSQEKDLLVELNNTLSK
                     VYDNPKSDAKNRVKILIETTEHLLDMENHSDPQLAKELCADLYALLCIAKRSLKKHET
                     LASWFIDVHEINKKLEELLAKTEEKVEILNKILSEKLNESLETSQKDRTIQEHFNERF
                     TEILSGDEKQRTKLDKLKMSLKEVAEGLSGIVGLRKEKATIGEKIKKIEALLKTVEEN
                     DKKVTGRQYFLDLLASHKDHFNTLMSTEGGELKKQLAAKIEQLKNPDFYQTVSSTFLW
                     GASWATSLGTVIYRQITPQAVQDRVSAYVPATLDSEAKALLQELARQQLIELKKQQSL
                     KRDELVQRYSQLSSGSPELEKLIINATSDHLEQIAAATTETSKVLDRYDQIEIKLKDN
                     VEKLKGNQLTRKQLSDFIEKNDTWIVKLSNWLAENIHEIFKSDTARMIDKARKAQQKL
                     GEFERQYQTEIESEKAKIENNDSLSSEMKVFLKNQFEETKKAGGMEQKPQKNLSKETL
                     QKLFVSLVPEHEEISSGVIFSPFN"
     gene            8196..>9288
                     /gene="legA2"
                     /locus_tag="U6309_002165"
     CDS             8196..>9288
                     /gene="legA2"
                     /locus_tag="U6309_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LegA2"
                     /protein_id="HEN5505603.1"
                     /translation="MSQSVDQSQVIDRLNRYLKWHNMPVSMNEEGICNGLVTVYAKYV
                     LEGKEKRFFELLDQIVRMEADSTIESEVNQFVYDVVLTLFPHQFNKKLSQANSMCALT
                     INNKPLVSSFDFAIATQDKNWEEIFKNLALQEDEIARVSGTAHAVAVRRKNNKYIVYD
                     PNYSSGIKEFSNEHELISELHYNVFGYHNGFYIKGGLLGMMVSVIRHPDNKKPRAFPQ
                     ITQLYDRYLTENNINDVAVSFHGGTFNTLEHAAMVSHAEVIKNLLEKGAKDNDHQAAG
                     NAVMYNNVDALKALLGKNRETELFQRLFHAALESGREEVYDALFSMKGALPFNSSVQA
                     IRAAAKGGNSVLLAKVMNYFKRNHTGFSDL"
BASE COUNT         3136 a         1505 c         1891 g         2756 t
ORIGIN      
        1 gacttacgaa aaaccccaat ctctttgtta aaaaatgctt ttaattcggt catttagttt
       61 atctaaactc cctcattaaa aacattattt gccttgacct tgaggttttt cgtaagtcct
      121 gacagactca aaagttgaca aatgaattgt ctattatttt caaggaaatc acaggatgac
      181 ggagcgcaag aaaacgacag tcaagaataa aaaagcacct gtcaaaaaaa gtcagctgac
      241 tgtttcttta ttgattttta ttgatttgat ttctatattg ctcagtttaa tgcctggcgt
      301 ggccgcattt agcttaaccg gtttgttttt gtattatggg ttttcaaaaa cctggcttgt
      361 tttaatttgc tcaccgtttg tattactttt ttcttttatt cttatcattg cacttatcag
      421 aataagcctt ccaaaactta agccaggtcg ctataaacga gagttaaata agaccatgat
      481 cgcctggttt tgtcattttg cactctctcg tgcagcgaaa attgttggcc tgattcattt
      541 attacaatcc tttaatgtta ttaaatattt gtattggcgt gcattgggag ttaaagtgtc
      601 atttcgcgtt atgaactcat ttgatatcga ttttgtggat tgccccctca tttcgattgg
      661 aaaggatgtt acccttgcca gtcaagtctc tatcagttgc cattctgatg taggaaatta
      721 tcttttttta tcgcctgtgg tgatagagga taatgttttt gttggaatga cgactaaaat
      781 tggcccagga acaacaatta aaaaaggggc ctggataggt tatgggaatg cttttgttaa
      841 ccagattatt gaggaaaatg caaaaattgg caatgttaga cctgttcctg aatagaatga
      901 aatgttttat cttaagtttg gattaataaa aatcagaatt tagtgcattg ttttcccggt
      961 taacttaatg aatccagatt gctgcaatca ggctttgaag ccgctgatta tttctttgat
     1021 taaacggatg ttccagaatg aaaaaaattg tttacgtctt gttatttatt tcttcgctga
     1081 ctcatgccaa ttgtgtgatc tattttaacg aacaaccggt taataaaagg ttatctgatc
     1141 ctttgtattc tctgttgtcc aatactgaaa tttgcccgca atcgattcag gagttaagaa
     1201 attccatgag attgagagga ttaaaagaag tcatagggat ggtcgctaat cgaggaagaa
     1261 ataatcctgg tgaaggcagt ttcagttttt ttgaatcagt gtatggtcga ttagcaaatg
     1321 accaattgat tgcaaagggg gagttatttc tggggtattt tactcgttta aaagatggcc
     1381 tagttatttt ggatcaaaaa cccaggaaag gtaaattatt gattgaagca atcagctggg
     1441 atgctaaaaa ggcgctttat aatttttatg aattgcgagg aatagaacat ggaggcgtac
     1501 gttggtttta tcgaggaaac tcgaaggatg catataaaga taacacctgg ttatatagaa
     1561 taagcccaga gggtgaaaat cattttggca gtcgtatgcg atgttcagct tgccataact
     1621 ccggcgggcc gatccttaaa gaacagtctc ttcctcataa tgattggtgg actaatcaac
     1681 gtccgcttat tttgctcccc aataaggccg atggagatgt gagccaatta ttggaacaag
     1741 tcagtgatgc cagtgtattt gcaaatgagg tgataacagg aatgcgacat ttgagcgata
     1801 gtaaaaaaat gcaggcattt caagggaaac tgtcattgca ggagcagtta cggcctttat
     1861 tttgtactac cgaaataaat ctccagtcca gttctgactc atcagaaacc gttgtatcca
     1921 ttccatcagc gttttggctt aatccactat taggcgacat taacttgacc atttcagctg
     1981 aagattataa tcaattattg aaagcgtttg gcactcgttt cccagaaacg tctttacaag
     2041 acgctgatca tgcctggctt acacctgtca aggcggaggc tgatctcaat gcgattaagc
     2101 aacttatcca acagaatata attagcaaaa aatttgcaca atccgtgctg atgatcgatt
     2161 ttaccaatcc tttgttttca tcaaaacgat gtgagttgtt aaaattaata ccggaatctt
     2221 atcaaacgaa cagcaaagaa tgggttgaac aatttctgat taatttaaga aacaatgagg
     2281 agcaatttaa aggggcacgt cagctagctg attatatgag taaagagcaa ttggattcaa
     2341 aagagctgat gaaaaaaatc aaaatttatg aatattttct aaaccagtct atatcaacca
     2401 gcacaggatt aaggaggcat tttcagcatt taatagagct gcgaaaagcg gtctttcgta
     2461 gtgaactgtc acaaaatccc ttagggcaaa ttttagagcc agggtttcga gtaattttcc
     2521 ctgaaattca gcagtagaca agaagaggtc tattattggc tctgtgtatg acaaaaaact
     2581 gtcatgcgct gagtattatt ttctacgaaa gagttgaggt gatgaaattg attgcatttc
     2641 ctccaccaat tgtgaactca acaggaatat agggcagatt acagagaaac agggtgacaa
     2701 tgaaaaatat tgctgtacct atatttccca tataaatcca ttgcttttcc tggttttcat
     2761 tcaaaaatgc tgtaatggca taagtgaagc tgaatagtac ggcaaccagt aagtaaatga
     2821 aagcatttgt atctagtgag aagtcggaaa ggatcttata agtcatcatt aaagccagaa
     2881 ggaacagaat gactcgatag actcttaaag ataattttaa ccaggaagga tagtctgatt
     2941 taatagttcc atcctgaaaa taggcattga aaaatatgat acctgctgta gtcagtggaa
     3001 ttagtactat taacgggttg atgtgttcct ggctgctcga aaaagaatga aatgtatata
     3061 atataaaata gaggatactt attgctgcca agaggggaaa caggtaatac attattctaa
     3121 gtaataaaaa tcgcatgtta tgaatgatat tgagattctg ttgaccaaca cccaatccca
     3181 taaaaaataa ggttatatta ctgattaatt taaaatcacg attataaaaa tacaaatccc
     3241 ataaataatt atttccaacg gttttaaata caaaagatcc caatacaata agtaaattgg
     3301 ccaatgagga aaagacgagg gcaataaaca aaagaggaat tgtattccat actccagcaa
     3361 ataaactgct gtaacttacc cgccaggtgt tgtcatgatg ataagcgtaa tggaaacaat
     3421 gagcgacgta gataaaaaat ggaaagccaa caaaaaaagt aaaaaaatgg aaatagtctt
     3481 tcattgagta gtccattttc aacggaaaca atggtattga tagaaagaaa gcgagcagga
     3541 agctggttcc aatcagacgc gaaaaattga cgttgttata agtaagggca aataaaacac
     3601 caaagatagt gacaacactg tatataaatg cagtcgtgtt gttgtagcgt atcagtatgt
     3661 ccactgctac ccctagaagt aatccaatca gggtaaaaat ataaaggtgt ctttgagcag
     3721 ccatcctgtc tttctccaca agggggttat attcatgatt tagggtttgg tagaatgccc
     3781 taatcgttat ctaaaaaata attctaactc agttacttaa ttgatctaaa tcgtttatta
     3841 aaaatatttt ttcgttccaa gtctgagcat gtgacagatc taatttcaat cattaagaaa
     3901 ctcacaaagt aatctttgaa aatgatgagt gccttgttct tttgttgggg agaaacgtcc
     3961 catatcataa tagcgggatt ttatccccct ttgcacagac tccaccactc tttcatcttc
     4021 ttgttcaacc tggtcaagtg ctccaccagc gcctttccca agttttgatt catcaagaac
     4081 aaaagataaa aaggaaacct gagttaactc aggactaagc ggtttgacta cattcacaga
     4141 acaaccccag ggataaaaat taagcatcgt attggggaat atccaaaagt aataggcagc
     4201 aacctgtttg ccataatctt gcgccccttt gggtaaatca aagctgactt catcagggct
     4261 tgccaaagcc aattgcagat tacagtagcg gaagagctct gtggtgtagg tgttgcaatc
     4321 gattactttt cttagcgagg gatgtacaaa agggatatgt aacgcttcaa ggtaattttc
     4381 acagtataag gcccaatgcg atttaactac ataatctttg catttttctt tatccaggcg
     4441 cattttttcc ataggcagcc aaaacaatct ttctttaatt tctgcaaata cttcatggaa
     4501 tgggacgatg ggggaaagtg aagcaaagag aaaaggttca agaatacccg ttgaaatttg
     4561 acttaggtta tcattgctcg atgggaaatc ttttgtgagt tcaaattcag gcatatgaat
     4621 taattcgcca caaaaattaa accgacggcc atgataagag cacttgattt tatgagctac
     4681 gcaaggttct tcgatcagga ttttacctcg atgggtacat acattactta aacaacgaat
     4741 tgtacccgat tcatcttttg taaacagcaa gggttcttca agaaatccag gcagcagggt
     4801 aaaaggaatt aattgtctat tggttgtcag ctcttcggta ctgatacaaa attgccaggt
     4861 tttactaaat atcttttctt ttgtaatttc aaaccattct gttgatgtat aaaattcaga
     4921 agagattgtg gaggcttttg taatatcagg atctatatag agattcttca tgaacatttc
     4981 cttactgtaa agagaaaaac ctgcaaaaag agaataaaca ttttaaccat ttctttggat
     5041 gaagcgatgg ttaaggtttg gtaaaaggta gtgcaaacct ttgtaccctg tcaacttgtt
     5101 tatttgataa caaaatgatt gattaattat cctaatgatg caaaaataaa cacagtttgg
     5161 gttttaagga actattgaat gagatctagc cggtttttat gcttattaaa tggcatgtta
     5221 ttatccagta atgttgcagc gttttcaccc tctgccccta tattcagccc ttatgttgat
     5281 ttaacgatta atactcattg ggactcgcaa tcacaggata tggagccaat ggatttgatt
     5341 tctccggcaa aaaaactggg gattaaagct tatcatttgg cttttattac agacagtggt
     5401 caatgccaac ctgcctgggg agcccagcaa aattacagtg tggcaaaggg atggggtaaa
     5461 aaacagttcg atgtgctatc aagagagggt ttgaaactca ccgtttcatt tggcggagcg
     5521 agcggtactg atatttccta tcattgtgat aaaaatcaat taattaatac gtttaatcaa
     5581 gtggttaatc aatatcatgc gaatgtcctg gattttgata tagaaaatgg aacagccaat
     5641 attcctaatt tgttgcaggc tttgaaatta ttccaaaagg agcatccgga tgttttgtta
     5701 agttttaccc ttcctgtgat gccggagggt ttaacttctg ttggaaaaga gataatcacc
     5761 tcagcggcta atctaggcct tcattttaat gtgaacataa tggctatgga ttacgggccg
     5821 gcttatagtg gagatatggg ggattatgcg atatccgcag caacgaatct ccatcaattt
     5881 ctgcaggaaa tttatccgga taaaaaaccg gaggctttat ggcagatgat tgaagtaacg
     5941 ccaatgatag gcgtgaatga tgtgaataca gagcaattta ccttatctaa tgcagcccaa
     6001 ttaaaacaat ttgcacagaa aaacctgctg gggggattgt caatgtggtc tttcaatcgg
     6061 gataaacctt gtgctgacaa atgggcaagt cctgtatgta gcggtaataa tttgcagagt
     6121 catgattatg aatttgtgaa atattttcag taatggaatt aattcaggat ttcctggttc
     6181 tgatgaagaa gaaatggttt gaataaatta tctatgttat ttttttgtca agataaacct
     6241 ggttttgatg aattaggtga tataattttc actatgataa attctaagaa acttattact
     6301 taatatttag ataataatag atgcatatac tggagttaag atttagcggg tatttctcca
     6361 tttattgttt ggaagagtgc taactggcaa ttagggttaa ttatgaagag taaagaagaa
     6421 cgatttaatg aatggcgcca agaaaaggcg aaatcagaac aggcatatat taaaactgtt
     6481 ccattagtgg aggaatacat taaatcattt gatacattga ctgagctcag aaatgatgtg
     6541 gctaatcctg attctgaaaa aattaaaaaa atggacgccc ataaaaagcg tactctgggt
     6601 gttttactct atgccaaatt aaaactggat gaagtaatag agaaaattcc ggattctgat
     6661 agtcaggaaa aggatttatt agttgaatta aataatactc tgagtaaagt gtatgataat
     6721 cccaagtcgg atgccaaaaa cagagtgaaa attttaatag aaacgacaga acacttattg
     6781 gatatggaaa atcactctga tccacaattg gctaaagagt tatgcgcaga tctatatgct
     6841 ctattatgta ttgcgaagag atctctgaag aaacatgaaa cgctggcaag ttggttcatc
     6901 gatgttcatg aaatcaataa aaaactggaa gaattattag caaaaacaga agaaaaagtc
     6961 gaaattttga acaaaatcct gtcagaaaaa ctcaatgaat cacttgagac ttcccaaaaa
     7021 gacagaacga tacaagaaca ttttaatgag cgattcaccg aaattttaag tggggatgag
     7081 aaacaaagaa caaaactgga taagttaaaa atgagcctaa aggaagtagc tgagggacta
     7141 tcgggtattg tggggttaag aaaagagaag gctacgattg gtgaaaaaat taaaaaaatt
     7201 gaagctttac tgaaaacagt agaagaaaac gataaaaaag ttacaggaag gcaatatttt
     7261 ttagacttac tagccagcca taaagaccat tttaatactc taatgagtac tgagggtggg
     7321 gagttaaaaa aacagttggc agcgaaaata gaacaactca agaacccgga tttttatcaa
     7381 actgtttctt cgaccttttt atggggcgca agctgggcta cttctttggg tacagtcatt
     7441 tatcgccaga taactcctca agcagttcag gatagagtta gcgcttatgt tcctgcaaca
     7501 ctggacagtg aagcaaaagc acttttgcag gaattggcaa gacaacagtt aattgagtta
     7561 aaaaaacaac agagtctcaa aagggatgaa ttggttcaga gatatagcca actatcaagc
     7621 ggtagccctg agttggaaaa actgataatc aacgccactt cagaccatct tgagcaaatt
     7681 gctgcagcaa caacagaaac aagcaaagtt cttgatagat atgatcaaat tgagattaaa
     7741 ctcaaagata atgtagaaaa acttaaggga aatcagttaa ctcgaaagca attatctgat
     7801 tttattgaaa aaaatgacac atggatagta aaattatcca actggcttgc agaaaatatc
     7861 cacgagatat ttaagtctga tacggctcgc atgattgata aggctagaaa agcacaacaa
     7921 aagctgggag aatttgaaag acaatatcaa acggaaattg aaagcgaaaa agcaaaaatt
     7981 gaaaataatg attcattatc ctcagagatg aaagtttttt taaaaaatca gtttgaagaa
     8041 acaaaaaaag caggtggaat ggagcagaaa ccacagaaaa atctcagcaa ggaaactctt
     8101 cagaaattat ttgttagcct ggtgcctgaa catgaagaga tctcatctgg ggtaatattc
     8161 agtccgttta attaatcagg gattagagcg atgatatgag tcaatcggtt gatcaaagtc
     8221 aagtcattga tcgattaaat cggtatctca aatggcataa tatgccagtt agcatgaatg
     8281 aagaaggaat ttgcaatggc ttggttaccg tgtacgcaaa atacgtactc gaaggtaaag
     8341 aaaagcgctt ttttgaattg cttgatcaga tagtcagaat ggaagcagac tcaacaatag
     8401 agtctgaggt aaatcagttt gtctatgatg tcgttctcac tttattcccg catcaattta
     8461 ataaaaaact aagtcaggct aattccatgt gtgccttgac aataaacaat aaacccttgg
     8521 tttcttcttt tgattttgca attgcaactc aagataaaaa ttgggaggaa atctttaaaa
     8581 atcttgctct gcaagaggat gagattgctc gtgttagcgg aacagcgcat gccgtagccg
     8641 tcaggagaaa aaataataaa tacattgtat acgatcctaa ttactcgtct ggtattaagg
     8701 agttctccaa tgaacacgaa ttgatttccg agttacatta caatgtattc gggtatcata
     8761 atggatttta tattaagggt ggtcttttgg ggatgatggt tagtgtcatc cgccatcccg
     8821 acaataagaa gccaagagct tttcctcaaa taactcagct ttatgatcgt tatttaaccg
     8881 aaaataatat taatgacgtc gctgtttcat ttcatggtgg aacatttaat actttagagc
     8941 atgctgcaat ggttagtcat gccgaagtaa taaaaaattt gcttgagaaa ggagcaaaag
     9001 ataatgatca tcaggctgct ggaaatgctg tcatgtacaa caatgtggac gcattaaaag
     9061 ctttactggg aaaaaataga gagacagaac tctttcaaag gctatttcac gcggctttgg
     9121 agagcgggag agaagaagtt tatgatgcct tgttttccat gaaaggtgcc ttgccattta
     9181 attcgtctgt tcaagccatt cgagctgcag caaaaggggg taattctgtt ctcttggcca
     9241 aggtaatgaa ttattttaaa agaaatcata ctggattttc agatcttg
//
LOCUS       DASLOE010000119         9191 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.120, whole genome shotgun sequence.
ACCESSION   DASLOE010000119 DASLOE010000000
VERSION     DASLOE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.120"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            136..546
                     /locus_tag="U6309_002166"
     CDS             136..546
                     /locus_tag="U6309_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEN5505604.1"
                     /translation="MRQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLTL
                     ADSAKLAVSETAITNNALPATQAATGYVSPAATPNVASIAIGANGVITITYTAAAGGG
                     TIVMTPTLQANGDVTWTCTGGTLLAKYRPASCRP"
     gene            739..1152
                     /locus_tag="U6309_002167"
     CDS             739..1152
                     /locus_tag="U6309_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEN5505605.1"
                     /translation="MRTKGFTLIELMIVAAILGILVAIAIPAYQDYTIRARVVEGLEM
                     ASAAKLAVVETSLIDNTLPANQADTGYTSPAPTINVASIAIGNQGIITITYTANAGNG
                     TITMVPTLDATNRILTWDCTGGTLISKYRPAYCRP"
     gene            complement(1149..2393)
                     /locus_tag="U6309_002168"
     CDS             complement(1149..2393)
                     /locus_tag="U6309_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEN5505606.1"
                     /translation="MSVKNAIYAQSGGVTAVINASACGVIQTAKLFPEQIGKVYAAKN
                     GIIGALNEELIDTSLESEEDIAKLMQTPSGAFGSCRYKLKDNGEEYQRLIAVFKAHNI
                     GYFFYNGGGDSQDTAHKVSQLGKTMGYPITCIGIPKTVDNDLPFTDNCPGFGSVAKYI
                     AISTKEAGFDVASMAASSTKVFILEVMGRHAGWIAAASGLAEQNPGEPPHIILLPEVP
                     FEPTRFLKKVKDCVNSYGYCVIVVSEGIRNKEGKFLSEAGLRDAFGHAQLGGVAPVIA
                     QLIKSELGFKYHWAVADYLQRAARHIASQVDLDQAYALGKAAVEYAVKGYNAIMPIVI
                     REQDEPYQWSIGQVPLAEVANQEKAMPPEYIAEDGMGITDACKRYLSPLIQGEAYPNY
                     KNGLPDYVRLKNQLVAKKINQD"
     gene            complement(2490..5222)
                     /locus_tag="U6309_002169"
     CDS             complement(2490..5222)
                     /locus_tag="U6309_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN5505607.1"
                     /translation="MLKSIGIKYQLRITTLIPAFLVALLFAFFYNGLFGKDLKQHMSR
                     LGEAYIRQLLPAAQYAMLRNDYRTLQGLINASTINPEVKALAFYNADGRLIAYRGGKH
                     SIHKPFNPPDYTGDYIESKQINPFTINFIAPITIPKFNLYSSTEFKELSTPKIFQADD
                     ILGWLSIDIDTQSLLIKRYQMLIVTIFITLFGLLMGLTIHYFLSKRIYMPIARLRRSM
                     KQILSNEFETEIRVSSPGELGIIEKGCAHLQRQYLNTVRDLNHHIEIATADLQQSLEL
                     LEEKNIELSLEKKKTEEKSRQKSEFIANMSHEIRTPMNGVIGFTNVLLESKLDPLQLD
                     YVKTIKSSAQDLLSIINDILDFSKIDAGKLNLDCIPLDIRGCIDEVLSLASPNAHKKG
                     IDLIPITDINVPKMVLGDPLRIKQIISNLVTNAVKFTDHGYVLIRTKIEQETDKDYTL
                     LFAITDTGIGISQEDQTKLFTAFNQADTSITRRYGGSGLGLVICKKLCEEMHGRISLT
                     SEINKGSTFSARIKVEKLVAYEIEKNQTHRFAHLKIICFDDNPLHLEAIGNGLGFWGI
                     EAIRVDSFNKLSRILTKHKDCKIAFINVNQGCERQAAELIAKHKQIPFVLISKWPIND
                     FAALGARGFLYKPISIQKLQDLIESIANENQTEKNTNQELDTLREQLRFLHPEILIAE
                     DNPVNKMLLTSLLNNNANITTVDDGEMAVTACEDKKFDMILLDLHMPKLNGLEAAKMI
                     RQKSLMNKHSPIVLITASSSDLSSIDMKKSGVDFCFQKPIDEKQLLIQILRIVDKTKH
                     AAIDWQLCVQKVSGNQALAEEFLAKFIEELYKNREEFIELMHQKNVKGLADLAHKLHG
                     ACCFCGVPILQKRVAQLEKLARRTANADNLTEAFTDLIQSIDAVISEYENQYSQ"
     gene            5321..6733
                     /gene="gltX"
                     /locus_tag="U6309_002170"
     CDS             5321..6733
                     /gene="gltX"
                     /locus_tag="U6309_002170"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEN5505608.1"
                     /translation="MTVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQFILRIEDT
                     DRERSTQESVQAILDGMAWLGLNFDEGPYYQTERYARYQQVAQQLLKEGKAYRCQCSK
                     ERLEALREAQLAAKEKPRYDGHCRNQTLPDSGVPYVIRFRNPDAGIVSFHDEVYGDIH
                     VDNSELDDLILVRSDGHPTYNFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKALDA
                     PVPVFAHLPMILGEDGKRLSKRHGAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIF
                     SVQEMITSFDLKNVSRGVSSFNYDKLYWLNQHYQKSDSPESVANALQWHFEQAGIDLN
                     QGPDLKDLVAVQAERCKSLAEMCQISQYFYTDTIEYNEDAVKKHLRPVVLEPLMVLHE
                     RLKALDEWKNDKIQECINDVSLQFDLNLGKIAQPLRVAVTGSGTSPSIDMTLALLGKN
                     KSIKRLEDALEKIRARASAV"
     gene            6979..8241
                     /locus_tag="U6309_002171"
     CDS             6979..8241
                     /locus_tag="U6309_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN5505609.1"
                     /translation="MKINNLVSFLLAFCLASLSYNISAAESPVVSHHKIQKEMSKYFK
                     VYHNKEKFTAIAASVFIPQEKNKDALDVATFVKGTMGYPPLSQEITPLNLFDIGSITK
                     SFTSLILLQLQTEGKLSLDDPLGRWLPQYKEWKDVKLRQLLNMTSGIPNYSEDPEFAK
                     KMEDNFAAIWGDEELLGYAHPEKPIETDRANRYEYSNSNYILAGMVIEKVTQDTFENQ
                     LKQRIINTNNGLNNSFYPAGPHGPEVEKSIRDRRVHGYFYDEDNKKLIDTISNDLSWA
                     GAAGAVIANTEDVGRWVQLLYHGLLIEPAFRDEALSELESVVSIKTGLPIPSVTEDEP
                     YGFGLGVGFYYDKNSRQRFWTYQGSTLGYRVMYLWNPCNNVTVVVALNGKGGSGNEKS
                     KMGNHIHELCLNIHKIIVKNHPHLQCAS"
     gene            8282..>9191
                     /locus_tag="U6309_002172"
     CDS             8282..>9191
                     /locus_tag="U6309_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505610.1"
                     /translation="MAICINKETDHFFISIGKINQHSFIMLGVYDDFQVSHLLCRVGK
                     IFDLPNQTKGIKRCLSIYSALGGAIFASSKAKIEDEGITRKRKGSVPISYQAYDISYE
                     QYCEFVHYLESIQTESNQFECFKPLVQNGNAVYFSQTSSRVFATGSHWKELNEEIHEI
                     NTGNTCRHSAIKLIEAVTKTSVPSSISSCFFINLPYKTQLDYGKPSQNIPFYVLPPPP
                     PSIHPGFNKEKCLIAKKLYHRIEQLPVLEPNSPMTKRKFNSLKNLYLQIIGSQKNQSI
                     NELLFGIQQWKEKNRVDLQTLRRTYFW"
BASE COUNT         2732 a         1697 c         1868 g         2894 t
ORIGIN      
        1 tttttagcac aaaaaacggt gttttttaca tgaaatgatt atttagcata taaaataata
       61 tcggtttaga atattggctg gtagttggca ttatgcttgc attatactaa tggatagtta
      121 actatggaga tggtcatgag acaaaagggt tttactttaa ttgaattgat gatagtcgta
      181 gcgattatcg gtattttggc agcaatcgct ataccagcct atcaagatta cacaatacga
      241 gctcgtgtta ctgaaggtct gaccttggca gattctgcaa aacttgccgt gtctgaaacg
      301 gctattacaa acaacgcgtt accagctact caggctgcaa cgggctatgt gtctcctgct
      361 gctacgccca atgtagcatc aattgctatt ggtgcaaacg gtgtgattac catcacgtat
      421 actgctgcag caggtggagg taccatagtt atgaccccaa ccttacaagc taacggtgat
      481 gtaacctgga cttgtacggg tggtactttg ttggctaaat atcgacctgc cagctgcaga
      541 ccataatagg ataaatcaac tataaaaacc gcctttcaat ggcggttttt tatttggcta
      601 agttctataa gctatttgcc tgacaggaat attttgagaa gaaagcttgt caagctattt
      661 agtcagcaga agattttaaa tcataaagtt ggcattaaat ttgcgtgata tcagttagat
      721 gcaatgccag gtggcgttat gagaacgaaa ggatttactt taatcgagct gatgatcgtt
      781 gccgctatac ttgggattct ggttgccatc gcaattccag cctatcagga ctataccatc
      841 agagcacggg tggttgaagg attggaaatg gcttcggccg ctaaattagc tgttgttgaa
      901 acgagtttaa ttgataacac tttgccagca aatcaggcag atacgggtta taccagtcca
      961 gccccaacaa ttaatgtggc ctctatagcc attggtaatc aaggcattat taccattacc
     1021 tacactgcaa acgcaggtaa tggcactatc acaatggttc ccaccctgga tgctactaac
     1081 agaatactca cttgggattg cactggcggt accctgatta gtaaatatcg tccagcctac
     1141 tgccgccctt aatcttgatt gattttttta gccactaact ggttttttag ccggacataa
     1201 tctggcaatc catttttgta atttggataa gcttcacctt gaatcaaagg ggacaagtaa
     1261 cgtttgcatg cgtccgtaat tcccatgcca tcttcagcta tatactctgg tggcatagct
     1321 ttttcctggt tagctacctc agccagaggg acttgaccta tagaccattg atagggttca
     1381 tcttgttcac gaatgacaat aggcataatc gcgttatatc ctttaacggc atattccaca
     1441 gcagctttgc ctaaagcgta agcttgatcc aaatcaactt gcgaggctat gtgccgggct
     1501 gcccgttgaa gataatctgc aactgcccaa tggtatttaa aacccagttc agatttaatt
     1561 aattgagcga tgacaggtgc aacaccacct aattgagcat gtccgaatgc atcacgtaat
     1621 cctgcttcac tgagaaattt tccttcctta ttgcggatac cttctgaaac aacaatcaca
     1681 caatagccat atgaattgac acagtccttc acttttttta gaaaacgagt tggctcaaaa
     1741 ggaacctcag gtaatagaat aatgtgtggc ggttcgcctg gattttgctc ggccagccca
     1801 ctagccgcag ctatccagcc tgcatgcctt cccatcactt caagaataaa cactttcgtg
     1861 gatgaagccg ccatggacgc aacatcaaaa ccagcttcct tcgttgaaat ggcaatgtat
     1921 ttcgcgactg atccaaaccc cgggcaattg tctgtaaaag gcagatcgtt atctactgtt
     1981 ttagggatac ctatgcaggt aataggatag cccatggttt tacctaactg ggatacttta
     2041 tgggcagtat cttgtgagtc cccgccgcca ttgtaaaaga aatagcctat gttgtgggcc
     2101 ttgaagacag cgattaagcg ttgatattct tccccattgt cttttaattt gtagcggcag
     2161 gagccaaaag ctccagaagg agtttgcatc aatttggcaa tgtcttcttc actttccaat
     2221 gaagtatcga ttaattcttc gtttaaggca ccgataatgc cattttttgc agcataaact
     2281 ttaccaattt gctctggaaa tagtttggcg gtttggatga caccacaagc tgatgcatta
     2341 attacagcgg tcacaccacc agactgggcg tagattgcat ttttaactga cattttgtct
     2401 ccataactag aatgatttta ttaagttgat acagattttt actttggttt tcatacaaga
     2461 gagtttttat ggccttattt cattaaatat tattgtgaat actgattttc atattcacta
     2521 atcactgcat caatactttg tattaaatcg gtaaaggctt cggttaaatt atcagcatta
     2581 gctgttcgtc ttgcaagctt ctctaattga gctactctct tttgtaaaat aggtacacca
     2641 caaaaacaac aggcgccatg taatttatga gccaaatcag ccaggccctt tacgtttttt
     2701 tgatgcatta actcaataaa ctcttcacga ttcttatata attcttctat gaatttggcg
     2761 aggaactctt cagcaagcgc ttgattacct gaaactttct gaacgcataa ttgccaatcg
     2821 atggcagcat gtttggtttt atcaacgatg cgaagaattt ggattaacaa ttgtttttca
     2881 tctataggtt tttgaaaaca aaaatcaact ccagattttt tcatatcgat ggagctcaga
     2941 tcactgctac tggcggtaat gagaacgata ggggagtgtt tattcattag ggatttttga
     3001 cgtatcattt tggctgcttc caaaccatta agttttggca tatgcagatc aagtaaaatc
     3061 atgtcaaatt ttttatcctc acaagcagta acggccattt cgccatcgtc aaccgtggtt
     3121 atattggcat tattgtttaa tagggatgtt aatagcattt tattgacagg gttgtcttct
     3181 gcaatcagta tctctggatg aagaaaacga agctgttcgc gtaaagtatc cagttcctgg
     3241 ttcgtatttt tctcggtttg gttttcattg gctatcgatt caatcagatc ctgaagcttt
     3301 tggatactaa taggtttgta aagaaaaccc ctggcaccaa gcgctgcaaa atcattaata
     3361 ggccatttgg aaattaacac gaaagggatt tgcttatgtt tggcaatgag ttcagcagct
     3421 tgtcgctcac atccttgatt gacgttaata aaggcaattt tgcaatcttt atgttttgtc
     3481 aatattcggg aaagtttatt aaatgagtca acgcgtatag cctctatacc ccaaaatccc
     3541 aagccattac ctattgcttc caaatggagg ggattgtcat caaagcaaat gatttttaaa
     3601 tgggcgaaac gatgagtttg atttttttca atctcataag caacaagttt ttcaacttta
     3661 atgcgcgcgc taaaagtaga gcctttgttg atttcactgg ttagactaat acgtccatgc
     3721 atttcttcac atagtttttt acatataacc aagcctaatc ccgagccacc gtaacggcgt
     3781 gtgatgctgg tgtctgcctg attgaaggca gtaaacagtt tcgtttgatc ctcttgtgaa
     3841 atacctattc ccgtatccgt aatggcaaat agcaaggtat aatctttatc tgtttcttgc
     3901 tcgattttag tgcgtataag aacgtatcca tgatctgtaa attttactgc attggtcacc
     3961 agattactga tgatttgttt gattcttaaa gggtcaccca ataccatttt gggcacattg
     4021 atgtctgtaa tcgggattaa atcgattcct tttttgtgcg cattggggct tgccagggat
     4081 aaaacctcat caatgcaacc tcttatgtct agcgggatgc aatcaagatt taatttgccc
     4141 gcatcaattt tagaaaaatc cagaatatca ttgatgatac ttaacaaatc ctgcgctgat
     4201 gatttgatcg ttttaacgta gtccagttgt aaagggtcca gcttgctttc caataaaacg
     4261 tttgtaaaac caatcacgcc attcattggg gtgcgaattt catggctcat gttggcaata
     4321 aattctgatt tttgtcgact tttttcttca gtttttttct tttccagtga tagctcaatg
     4381 tttttttctt ccagaagttc tagactttgt tgcagatctg ctgtggcgat ttcaatgtgg
     4441 tgattcaaat cacgaactgt atttaaatat tgtcgttgta aatgagcgca acctttttca
     4501 attattccta attctcctgg acttgaaaca cgaatttcag tttcaaattc attacttaaa
     4561 atttgcttca tacttcgacg caatcgagca ataggcatgt aaattcgttt agacaagaag
     4621 taatgaattg ttaaacccat cagtaaaccg aatagggtaa taaaaatggt aacgatgagc
     4681 atttgatagc gttttataag tagtgattgt gtatcaatgt caatagataa ccagcctaat
     4741 atatcgtcag cctggaatat tttaggcgta gaaagctctt tgaactctgt gctggagtac
     4801 aaattgaact ttgggatagt aattggtgct ataaaattaa tggtgaatgg gttaatttgc
     4861 ttactttcaa tgtagtcacc tgtatagtca ggtgggttga atggtttatg aatagaatgc
     4921 ttgccaccac gataagcaat taatcgacca tcagcattat aaaatgccaa agcttttacc
     4981 tccgggttaa tggttgaggc attgattaaa ccctgcaatg tacggtaatc attgcgaagc
     5041 atggcatact gggctgctgg aagtaattgc ctaatatagg cttcacccag tctggacata
     5101 tgctgcttta gatctttacc aaataggccg ttataaaaaa aagcaaaaag caaggcgact
     5161 aaaaaggcag gaataagagt agtgattctt agctgatatt taataccaat acttttcaac
     5221 atagctcctt ttagtcttaa tgcagcagtt gaatctttgc gttcatggta aactcggcta
     5281 ttataacttg attattttat tccttacaat ggaagatatt atgactgtta gaacccgatt
     5341 tgccccaagc cctactggtt ttttacacgt tggaggggta aggacagctt tattttcatg
     5401 gctatatgca aagcatcaca acggccaatt tatactgcgt atagaagata cggacagaga
     5461 gcgctcaact caagaatctg tacaggctat tttagacgga atggcctggt tagggcttaa
     5521 cttcgatgaa ggtccctatt atcagactga gcgatatgct cgttatcaac aagtagcgca
     5581 gcaacttttg aaagaaggaa aagcttatcg ctgtcaatgc agtaaggaga gactggaagc
     5641 tttaagagag gctcaattag ccgccaagga aaagccacgt tatgacgggc attgcagaaa
     5701 ccaaactctt cctgatagtg gagttcctta tgttattcgc tttagaaatc ctgatgcggg
     5761 tatagtgtct ttccacgatg aggtttatgg tgacattcat gtggataata gcgaactgga
     5821 tgatttgatt ttggttcgtt ctgatgggca ccccacctat aattttgctg tagttatcga
     5881 tgattgggat atgaaaataa cccatgttat tcgcggggat gatcatatca ataatactcc
     5941 gagacaaatt aatttattca aggcattgga tgcgccggta cctgtttttg cccatttgcc
     6001 catgattttg ggtgaagatg gcaaacggct gtcaaagcga catggtgcag taagtgtctt
     6061 gcagttcaag gagctgggtg tattgcctca tgctttattg aattatttag ttcgtttagg
     6121 atggtctcat ggagatcagg aaatttttag tgtacaagaa atgataacca gctttgactt
     6181 gaaaaatgtc agtcgaggtg tctcaagttt caattatgat aagttgtatt ggttgaatca
     6241 gcattatcaa aaaagtgact ctccagaatc agttgccaat gcgttgcaat ggcattttga
     6301 gcaggcagga attgatttga atcaaggtcc tgatttgaag gatttggtcg cagttcaggc
     6361 agagagatgt aagtcattag cagaaatgtg tcaaattagt cagtattttt atacagatac
     6421 cattgaatat aatgaagatg ctgttaaaaa acacttgcgg ccagttgttc tggagccttt
     6481 aatggtattg catgagcgcc taaaagcgct tgacgaatgg aaaaatgata aaattcaaga
     6541 atgcattaat gatgtgagcc tgcaatttga tttgaattta ggcaaaatcg ctcaaccgtt
     6601 gcgtgtcgcc gtgaccggat ccggaacatc tccttccatt gacatgacat tagctttgtt
     6661 aggtaaaaac aaatcaatca aacgtcttga agacgctttg gagaaaatac gagccagagc
     6721 cagtgctgtt tagaatctaa atatttattg attttaagct gaatgcaaaa agatctgtat
     6781 ttggagaagt attcatattt caagtgggca atgaaaacta caatttcgct ggatttcatt
     6841 gctcctggcc gaagagaatg cgaataggcg tgtccgtata ttcaacagaa catctcctgt
     6901 ttttttattt tatggatcta tttgagtcta tcaggttaaa atggccaatt ttctacttaa
     6961 cttgatgtat ttttcgccat gaaaataaat aatttagtta gttttctgct tgccttctgc
     7021 ctggcttctt tatcttataa tatcagcgca gctgaatctc ctgttgtatc tcatcataaa
     7081 attcaaaagg aaatgagcaa gtacttcaag gtttatcata acaaagaaaa atttactgcc
     7141 attgctgcct cagtgtttat tcctcaagag aaaaataagg atgcattgga tgttgccact
     7201 tttgtcaaag gaactatggg atatcctcca ctgtctcagg aaattacccc attgaatttg
     7261 tttgatattg gaagcattac caaatcattc acttcattga ttttgcttca attgcagact
     7321 gagggcaaat tgtcactgga tgatcctttg ggtaggtggt tgcctcaata caaagagtgg
     7381 aaagatgtca aattaagaca attattgaat atgaccagtg gcatccccaa ttattccgaa
     7441 gatcctgaat ttgcaaaaaa gatggaggat aattttgcgg caatctgggg cgatgaggag
     7501 ttgttaggtt atgctcatcc agaaaagcca atagaaaccg acagagcaaa caggtatgag
     7561 tattccaatt ctaattatat tcttgctgga atggttattg aaaaagtgac tcaagatact
     7621 ttcgaaaatc agttaaaaca aaggattatt aatacaaaca atggattaaa caattcattt
     7681 tatcctgcag gaccacatgg tccagaagtc gaaaaatcaa tccgggatag acgagtccat
     7741 ggttattttt acgatgagga caacaaaaaa ctaattgata ctataagcaa cgatttatcc
     7801 tgggctggcg cagcaggtgc tgttatcgca aatactgaag acgtcggtcg ttgggtgcag
     7861 ctgctatacc atgggttact gatagaacct gcttttagag acgaggcttt atccgaattg
     7921 gagtcagtgg tttcaataaa aacagggtta cctatccctt cagtcacaga agatgagccc
     7981 tacggttttg gattgggagt tggtttttat tatgataaaa attccaggca gcgcttttgg
     8041 acttaccagg gcagtacttt aggttacagg gtgatgtatt tgtggaatcc ttgtaataat
     8101 gtaacggttg ttgtggcttt gaatgggaaa ggtggctcag gcaatgagaa atcaaaaatg
     8161 ggcaaccata tccatgagct ttgtttgaat atacataaaa ttatcgttaa aaaccatcca
     8221 cacttacaat gtgcaagcta atttattatt tatcattatt ggttgttaag gactgaatca
     8281 catggcaatc tgtattaata aagaaacaga tcactttttt atttcgatag gtaaaatcaa
     8341 tcagcactca tttattatgc taggtgtgta tgatgacttc caggtatctc atctactgtg
     8401 cagagtagga aaaatctttg atctgcctaa tcaaaccaag ggtattaaaa ggtgtcttag
     8461 catttatagt gctttaggtg gggcaatttt tgcgagtagc aaagcaaaga tagaggatga
     8521 aggaattaca agaaaacgaa aaggatctgt acctatttct tatcaagcct atgatattag
     8581 ttatgagcag tattgtgaat ttgttcatta tcttgagtct attcaaactg agagcaatca
     8641 atttgaatgt tttaaaccgc ttgttcaaaa tggaaatgca gtctattttt cgcaaaccag
     8701 ttctcgtgtt ttcgcaactg gttcccattg gaaggagctt aatgaggaaa ttcatgaaat
     8761 caatacaggc aatacttgtc gacattcagc aataaaatta attgaggcgg ttacaaaaac
     8821 atctgtcccc tcatcgatat cctcttgttt ctttataaac ctgccgtata aaactcaatt
     8881 ggactatggc aaaccgagtc aaaatattcc cttttatgtt ttacccccac ctccaccttc
     8941 tatccatcca ggatttaata aagaaaaatg cttaatagcc aaaaaactct atcatagaat
     9001 agagcagttg cctgtgctcg agcctaattc accaatgaca aaacgaaaat ttaatagctt
     9061 aaaaaattta tatttgcaaa tcattggttc acagaaaaac caatctatta atgaattgct
     9121 gtttggtata cagcaatgga aggaaaaaaa tcgagttgat ttgcaaacat taagaagaac
     9181 ttatttttgg g
//
LOCUS       DASLOE010000120         9076 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.121, whole genome shotgun sequence.
ACCESSION   DASLOE010000120 DASLOE010000000
VERSION     DASLOE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.121"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            128..1750
                     /locus_tag="U6309_002173"
     CDS             128..1750
                     /locus_tag="U6309_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEN5505611.1"
                     /translation="MVYYINLKEQPLPQDKEILTQLIKQYLLSGSSQSALFAKHHYTL
                     NDENGSEIEFLLSHDIYKNQRHDGITEFLILNGPKIGSGTKGEVRQSNHAMYLMYDEL
                     VIFPKENVLKVLYRNFSNAEELIKQESANYSILSGNSAAELVNLQGGSQGFFMQKEEG
                     CTLEELLEDIDSLQKLTFSDKMELAISFLTELENLHNKGFVHGDLTFKNILYNEESKR
                     LKIIDAGEMKVADERFLAQDILIARDMVSLLFTTDEANQFKASSYWSDEPTIRRIVNP
                     NLVETKLNRILNSKYSCISEFKSAIEWNYNEYLSQLNSLLQRNEISKMEREASNLQAY
                     KDQMNKQQLLGEIRESKEVFLNMKESLPLADQYRLKVFSDYLDNICSALNHDKTNEKL
                     ARAAYDFLTAQINKLFEDNGKNSQLSFKSFSKDVQQFLNHVNTFLIKYPSSSSNSIAS
                     IIQLFKQLDNQASFEQEQSLKTFSGYKEIMIQLLLKPFETPVAEIASAPATVSDFIEK
                     QSDTKRRLQEMKSSVNQDKQPIFTPSLMRGPS"
     gene            2109..3422
                     /locus_tag="U6309_002174"
     CDS             2109..3422
                     /locus_tag="U6309_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEN5505612.1"
                     /translation="MEYHRVWRFSYGLVLLLLYASLATAGNALTLKEVTQIALANNKD
                     LKAARYNVSIAKARLLQAGFWPNPSLNLSNNDDRLFTDEGEYSRSAGFSQAFPISGRI
                     AKQKTVARLDVLKAITEIREAERQLSAKVASAFYTAVITEHRIQQVNYLLRINQELVD
                     VIHNRYHAAEISKLDENAARIEYLRIVQEKHLLHSLSISQYATLNQLMGREANSSLSL
                     KMDFAAAKKLPALDALKTQALKNRPDRQSIALSIERANADRRLAKAERFADWTVGLGI
                     QQDKIVVEEGPPQPADRTLGVTLSVPLPLLNRNQGRILEASHTGTQAMMSLRALDLSI
                     ATEIASNYEQLKALSKSLEETQRVSLQLTIENVKLARDSYENGQISFLNVLQVQKQQN
                     DLQTTYLNTLEKFLQVYVALCTAIGPGKTDGFCSYLEYQSNGDSK"
     gene            3419..4387
                     /locus_tag="U6309_002175"
     CDS             3419..4387
                     /locus_tag="U6309_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEN5505613.1"
                     /translation="MRLQLKIRMMIMTVFFLGLLSPMQVSFAHGGNIEVSEGGTKGPV
                     HLTLEQARMLDIKVVQATHRPMAQLLGLNGQIQLLPDAQADVSIRISGSVTAIDVNLG
                     DSVKAGQRLATVQSRLVGNPPPSVAVNAPIAGIIDARNINLGQAVEPNTVLFHISNRD
                     KLLVVAQVYEEDLGKVKVGQEVNVHVLSYPKQTFPGQVTLIEPNLDPLTRTVNVRITL
                     DNKEGLLKPGMFVRANVILTHNKAALTVPNAALLQADNQQFVFVQNGGTYKRVDVKVG
                     AVEDDYSEIIGGLVPGDLVVTQGNRELYTLWLSGGRKLQSGQEEHH"
     gene            4388..7588
                     /locus_tag="U6309_002176"
     CDS             4388..7588
                     /locus_tag="U6309_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEN5505614.1"
                     /translation="MFTRLIAWSLHNRLLILALTIILCLLGGYTLKQMPVDVFPEFAP
                     PQVVVQTQAPGMATQDVETLITYPLESAINGTPGVASVRSKTSVGLSTITVVFDDKTD
                     IYLNRQLVNERIQQVVNRLPPGVSSPVMLPVTSAVGWLLKYALVSHTASPETLRTISD
                     WTIRPRLLALGGVASVVSLGGEVKQYQVQLDPKRMLAYGITVEEVRQALATGNQNVPG
                     AFVHQGGTEFVVSTIGRIKTLDDIKKTLIVVRKGVPVTVNNVATVAFGGEIKRGDASF
                     NAEHAVIGTVSKIYGADTLTTTLKVEQALADIKKSLPAGIELNTEVFRQANFIESAIH
                     NLTKALIEGAVIVIAVLFLFLMNWRASFITFLSMPVSFIVGILVLHYFGFGINSMTLG
                     GMAIAIGEVVDDGIITVENVVHRLRLNRLAENPLPAIEIVFDAVLEIRSSVVYATIII
                     SLVFLPIFFLSGIPERIFSPLAIAYIASVLGSLVVSITMVPALCYILLARKTEKNQDA
                     KVAVHALSEDERYHCTQEETVKQAQTETRFVIWLKKHFLTGLNWSLAHCKTVVAISIA
                     AFVLALSLLPFFGTSFLPEFHEGNFIIVMSTLPGTSLNESMRLGKQVSKTLLKYPQVI
                     SIAQRAGRSELDEDALPPNISEFDVHLNFGKDKTMSPDELLQSIRADLANIPGALFNV
                     GQFIAHRMDEVLSGVRAQVAVKLFGDNLATLNELGESVESILKTVPGVVDINKEQQIN
                     VPQLVIKIDREKAARYGVNIGQISEDIQILLNGVTVSSILEGQRTFDLYLRMGETERD
                     SVKAIQNMLIDAHGAANNQETKIPLRAVADIQLEPQPFAINRENVQRVLVIGFNVQGK
                     DLGSVIQEVQHQIKEKISLPGGYFVQYGGQFESQQQASRVIVLFGALAVFIMLVLLHK
                     AFGTFREALLVMFNLPLALIGGVVSLFLASGDMSVAAMIGFITLFGIAARNGIILVSH
                     YNQLRSLGREREQVVIQGTLDRLVPVLMTAATAALALIPLLWGSPAGKELQRPLAQVL
                     LGGLLTSTVLNMLVVPTVYNAFEIWHEKRRQLKEIS"
     gene            complement(7695..8951)
                     /locus_tag="U6309_002177"
     CDS             complement(7695..8951)
                     /locus_tag="U6309_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5505615.1"
                     /translation="MENKKKRVVVTTAGTFLEWAEFTYYAYIANIISGSFFPKLGNHL
                     GLIATFSVFALSYFFRPLGALFFGYLGDNMGRRVALQSSIMLMGLSSLCIGCLPAYDS
                     IGILAPILLLIFRCVQGFAVSGEFNGSAIYLIEYDSQKPCQAGSWTGFASALGMMFGG
                     LMSTLIYLPYMPEWAWRIPFLLGTLSCFCAMYFRKNLSESPPYLTLSKEKSSNPLKIL
                     FENYKKQLIRAVLLIAALGIYIYIMNVYYASHLAKYTTLSEAQTKFVVTLGQGLVVLF
                     IVLTGMYADRIDERHIIKTGLWGYFIAAPVAFLIPQTNSFTLILLSQIPYALCNALVG
                     TPIFKLLNDFFPTQIRYSGVSVAWGVSMAIFGGTAPLVANYLQYSFKLTTAPIFYILL
                     SASIALFILHDKKSYKITSIKLRSDN"
BASE COUNT         2791 a         1649 c         1893 g         2743 t
ORIGIN      
        1 catcagtaat tcattgaaaa ataatagata taactaaatt taataaaaca tatcaaaaca
       61 attaattgta ttaatattaa tcaatatgct caaatataag tagctgattg gcttatagga
      121 gagcattatg gtttattaca taaatttgaa ggaacaacct ttacctcaag ataaagaaat
      181 tttaacgcag ttaattaagc aatatttatt gtccggttcc agtcagtccg cattgtttgc
      241 taaacatcat tacaccctaa acgatgaaaa tggaagtgaa atagagttcc tcttgagtca
      301 tgatatatac aagaatcaac gtcatgatgg tattactgag tttttaattt taaatggtcc
      361 aaagataggg agcggaacca aaggcgaagt gagacagagt aatcatgcta tgtatttaat
      421 gtatgatgaa ttggtaattt tccctaaaga gaatgtactg aaggttttat atcgaaattt
      481 tagcaatgcc gaggagctaa taaaacagga atctgccaat tattcgattt tatcaggtaa
      541 ttcagccgca gaattagtca atttgcaagg aggaagccaa ggttttttta tgcaaaaaga
      601 agaagggtgt actttggaag agcttcttga ggacatagac tcacttcaaa aattgacatt
      661 ttcagacaaa atggagttag ccatttcatt tttaactgag ttagaaaatc tgcataacaa
      721 aggctttgtc catggcgatt taactttcaa aaatatttta tataacgaag agagtaagcg
      781 attaaaaatc attgatgcag gagagatgaa agtagcagat gaacgctttt tagcgcagga
      841 tattttaatt gccagggaca tggtatctct cttatttaca acggatgaag caaatcaatt
      901 taaagcatcg agctattggt cagatgagcc cacaatcagg cgaatagtta acccaaatct
      961 ggtcgaaacc aaattaaata gaattttgaa cagtaagtac agttgtattt cagagttcaa
     1021 gtcggctatt gaatggaatt acaatgaata tctaagccaa ttgaattctc tattgcaacg
     1081 aaatgagatt tcaaaaatgg aaagagaagc ttcgaattta caggcctaca aggatcaaat
     1141 gaataagcaa caactcttgg gggaaattcg agaatctaag gaagtttttt taaatatgaa
     1201 agaaagcttg cctcttgcag atcaataccg tcttaaagta ttttctgatt atctggataa
     1261 tatctgctcc gcattaaatc atgataaaac caatgaaaag ttggccagag cggcttatga
     1321 ttttctaaca gcacaaatca ataaattgtt tgaggataat gggaaaaact cacaattaag
     1381 cttcaaaagc ttctcaaaag atgtgcagca attcttaaat catgtgaata cctttttaat
     1441 aaaataccca tcttcctcct ctaattcgat agcttccatc attcaactat ttaaacagtt
     1501 ggataatcaa gcgagttttg agcaggaaca atcgcttaag actttctctg gatataaaga
     1561 aattatgatt caattgttat taaaaccatt tgaaacacca gtggctgaaa tagctagtgc
     1621 accggctaca gtatctgatt tcattgaaaa acaatctgac accaaaagaa gacttcaaga
     1681 aatgaaatct tctgtaaatc aagataagca acctatattt accccttcat tgatgcgagg
     1741 cccaagctaa aaaatgtcac tgaagtgtta gagcagcctg tcgtctatta ttttactgct
     1801 cttgtgtaat tacatttgct gggagatact aacaggactt acgaaaaatc ccaatctcta
     1861 tgttaaaaaa tgtttttaat tcggtcattt agtttatcta aactccctca ttaaaaacat
     1921 tttctgcctc aaccttgggt tttttcctaa gtcctgaata aatcatacaa caataacaag
     1981 aaggtattaa ttgatgactt actttcgaat aaaacacagt ccattactct tgatgtttgt
     2041 atctgccaca gattattttt tcgattccat gttgactaaa gatagataga ttttttgagc
     2101 tatcgtttat ggaatatcat cgtgtttggc ggttttctta tggattagtc ctgctactgc
     2161 tgtatgcctc tctggcaacg gcggggaacg ctttgactct taaagaagta acccaaattg
     2221 ctttagcaaa caacaaggat ttaaaagctg cccgttacaa tgtatctatc gcaaaagccc
     2281 ggttgctaca agcaggtttt tggcctaatc caagtttgaa tctttcaaat aatgacgatc
     2341 gtttattcac tgatgaagga gagtattcgc gcagtgctgg ctttagccag gcatttccca
     2401 tatcaggacg tattgcaaaa caaaagacag tggcgcgtct ggatgtgttg aaagccatta
     2461 cagaaatcag agaagctgag cgccaactca gtgccaaagt ggctagtgcc ttttatacgg
     2521 cagtgattac agaacatcgc atacagcaag tcaattactt attacggatt aaccaggagt
     2581 tggttgacgt tatccataat cgatatcatg ccgcagaaat ttcaaaattg gatgaaaatg
     2641 cggcacgcat tgagtatttg cgtattgttc aagaaaaaca tcttttgcac agccttagta
     2701 ttagtcaata tgcgacacta aatcaattaa tgggacgtga agccaactct tctttatcac
     2761 ttaaaatgga ttttgccgct gcaaaaaaac tcccggcatt agatgctcta aaaacacagg
     2821 cgcttaaaaa tcgccctgat cggcaatcta ttgcgttatc gattgagcgt gccaatgctg
     2881 atcgccgttt ggcaaaagcc gagcgctttg cagattggac agtaggcctt ggtattcagc
     2941 aggataaaat tgtggtggaa gaaggtcccc ctcaaccagc cgatcgtact ttgggcgtta
     3001 cactgtctgt tcctctccct cttttaaatc gaaatcaagg gcgaatttta gaagcaagtc
     3061 atacaggtac tcaggcgatg atgtcattac gtgccctcga tttaagtatt gcaacggaga
     3121 tagccagtaa ttatgagcaa ctcaaagcat taagtaagag tcttgaggaa acacaacgtg
     3181 tttctcttca attaactatt gaaaatgtaa aattggcccg tgattcttat gaaaatgggc
     3241 aaatttcttt tttaaatgtg ctacaagtgc aaaaacaaca aaacgatttg caaaccactt
     3301 atctcaatac gttagaaaaa tttttacaag tctatgtggc cttgtgtaca gctattggtc
     3361 ctggaaaaac agatgggttt tgctcttatt tagagtatca aagcaacggt gatagcaaat
     3421 gagattgcag cttaaaatta gaatgatgat catgactgtt ttttttcttg gtttactttc
     3481 ccccatgcaa gtgagttttg cacacggagg aaacatagaa gtaagcgaag gaggcaccaa
     3541 agggccggtt catttgactc tggaacaagc caggatgctt gatatcaaag ttgtccaggc
     3601 aacccatcgt cctatggcac aattgctggg tttaaatggt caaattcaat tattacctga
     3661 tgctcaagct gatgtcagca ttcgtattag tggcagtgtg acagcgattg atgtgaattt
     3721 gggggatagt gtcaaggcag gacagcgctt ggctacagtg caatcacgat tggtgggaaa
     3781 tcctccaccg agcgttgctg tcaatgcccc aattgctggt atcattgatg ctaggaacat
     3841 caatttaggt caggctgtcg agccaaatac cgttcttttt catatcagta atcgcgataa
     3901 attattagtc gtagctcaag tgtatgaaga agatttaggt aaagtgaagg tagggcaaga
     3961 agtgaatgtt catgtcttaa gttatcccaa acagacattc ccgggtcaag tcactttaat
     4021 tgaacccaat cttgatccat tgacccgtac agttaatgta cgaattactt tggataacaa
     4081 ggaggggcta ctgaaacccg gaatgtttgt gcgggccaat gttattttaa ctcacaataa
     4141 ggcagcactc actgtgccaa atgctgctct tttacaagca gataaccagc aatttgtctt
     4201 tgtgcaaaat ggaggcactt acaaacgagt cgatgttaaa gtaggcgctg tggaggatga
     4261 ctactcagaa attattggtg gtctagttcc tggcgatctc gttgttactc agggaaatcg
     4321 cgagctttat acactttggt tgagtggagg ccgcaaactg caatcagggc aagaggagca
     4381 tcattaaatg tttactcgcc ttattgcctg gtctttgcat aatcgtctct tgattttggc
     4441 attaacgatt attctctgtt tgctgggtgg ttatactcta aagcaaatgc cggtagatgt
     4501 ttttcctgaa tttgcccctc ctcaagttgt agttcaaacc caagcgccag gcatggcaac
     4561 ccaagatgtg gaaacattaa tcacttatcc tttggaaagt gccatcaatg ggactccagg
     4621 ggtagccagt gttcgctcaa aaacctcagt cggattatca acaattaccg tggtatttga
     4681 tgataaaacg gatatctatc tcaatcgtca actggttaat gagcgcattc aacaggtagt
     4741 caaccgtctc cccccaggcg tttcctcacc tgtgatgctg ccagtcacat cggcagtagg
     4801 gtggttgctt aaatatgctt tggtgagtca cacagcaagc cctgaaacct tgcgcactat
     4861 ttctgattgg actattcgtc cacgtctatt ggctttaggc ggagttgctt cagtagtttc
     4921 actgggcgga gaagtaaagc aatatcaagt acagcttgat cccaagcgta tgctggctta
     4981 tggcatcaca gtcgaagaag ttcgtcaagc gttggcaaca ggaaatcaaa atgtacctgg
     5041 tgctttcgta catcaggggg gcacggaatt tgtcgtaagt accattggtc gtattaagac
     5101 gttggacgat ataaaaaaaa cactgattgt agtccgcaag ggagttcctg ttactgttaa
     5161 taatgtagcc acagtcgctt ttggcggtga aattaaacgc ggtgatgctt ctttcaatgc
     5221 agaacatgct gtgattggta ctgtttccaa aatttatggc gctgacacat taaccacaac
     5281 acttaaagtg gagcaagctt tagctgatat aaaaaaatcc ttgccggcag gtattgaatt
     5341 aaatacggaa gtatttcgcc aagccaattt cattgaatca gcgattcata atttaaccaa
     5401 agctcttatt gagggtgctg ttattgtgat tgctgtattg tttctttttt tgatgaattg
     5461 gcgagcctcg tttattacct ttctctctat gcccgtttcc tttatcgtag ggatcttggt
     5521 ccttcattat ttcggttttg gtattaactc catgactttg ggaggaatgg cgattgctat
     5581 tggtgaggtg gtggatgatg ggattattac cgtagaaaat gtggtacatc gtttacgact
     5641 taatcgtttg gcagagaacc ccttgcctgc gattgaaatt gtttttgatg cggttttgga
     5701 aattcgcagt tcagtggttt atgcgaccat tatcattagc ctggtatttt tacccatctt
     5761 ctttttgtca ggaattcccg agcggatttt tagcccatta gctattgctt atattgcctc
     5821 cgtattaggt tctcttgtcg tttctattac tatggtacca gccctatgtt atatcctttt
     5881 agcgcgaaag actgaaaaga atcaggatgc taaagtggct gtgcatgcct tatctgaaga
     5941 cgagcgttat cattgtacgc aagaggaaac ggttaagcag gctcaaacgg aaacgcgctt
     6001 tgtgatttgg cttaaaaagc attttttaac tggtttaaat tggtcactgg cccattgtaa
     6061 aacagtcgta gccatctcaa tcgcagcctt tgttcttgct ttaagtttac ttcctttttt
     6121 tggaacttct tttttgcctg aatttcatga aggcaacttt attattgtga tgagtactct
     6181 tccgggaact tcattgaatg aatccatgcg cttggggaag caagtcagta agactttgtt
     6241 gaaatatccg caagtgattt ctattgccca aagagcaggg cgcagtgaat tggatgaaga
     6301 tgctttaccg ccgaatatta gtgaattcga tgtacatctg aattttggta aagataaaac
     6361 catgtctcct gatgaattgt tacaatctat tcgagctgat ttagcgaata ttccaggagc
     6421 gttatttaat gtggggcagt ttattgccca tcgcatggat gaagtgttat caggagtcag
     6481 agcgcaagta gctgtaaaac tctttggcga taatcttgct acccttaatg aattgggtga
     6541 atcggtagaa tccattttaa aaaccgtccc gggtgtagta gatattaata aagagcagca
     6601 aatcaatgta cctcagctgg tcattaaaat tgatagagag aaagcggctc gctatggtgt
     6661 taatattggt caaatttccg aggatattca aatccttctt aatggtgtga cggtatccag
     6721 tattcttgaa ggccagagga cgtttgattt gtatttgcgt atgggtgaaa cagagcggga
     6781 tagtgttaaa gccattcaaa atatgctgat tgatgcccat ggtgcggcca ataatcaaga
     6841 aacaaaaatc cctttgcgtg cggtggctga tattcagtta gagcctcaac cgtttgcaat
     6901 taatcgagaa aacgtgcaac gtgttttggt tatcggtttt aatgtgcaag ggaaagattt
     6961 gggtagtgtg attcaagaag tacaacatca gattaaagaa aagatttcgt tacctggtgg
     7021 ttatttcgtt caatatggcg gccagtttga aagtcaacaa caagcatctc gtgtgattgt
     7081 tctttttggt gcgctggcag tttttattat gttggtgctt cttcataagg ctttcggcac
     7141 atttagggaa gctctattgg ttatgttcaa cttacccttg gcattgattg gtggagtcgt
     7201 ttctttattc cttgccagcg gtgacatgag tgtcgctgcc atgattggtt ttattactct
     7261 gtttgggatt gcagcacgta atgggattat tttagtgagc cactataatc aattgcgctc
     7321 tctggggcga gaacgtgaac aagtggttat ccagggaacc cttgatcgcc tggtacccgt
     7381 gttaatgaca gcagcgacag cggctctggc cttaatacct ctattgtggg gttctcctgc
     7441 cggtaaagaa ctccagcgtc ctttggcgca ggtgctatta gggggattat tgacttctac
     7501 ggttttaaat atgcttgtag tacctacagt ttataatgct tttgaaattt ggcacgaaaa
     7561 gagaagacaa ttgaaagaaa tctcttaatc agagccatga taactctaac aatgggggct
     7621 attaagaagc aattgtcaca aattgattag atgtttgcgt agacatgttt ttgcaatggt
     7681 gataagtagt ttattcaatt atctgatctt aattttatag aggttatctt ataactcttt
     7741 ttatcatgca aaatgaacaa ggcaatactg gcagataaaa gtatataaaa tattggcgca
     7801 gttgttaatt tgaatgaata ttgtaaataa ttggcaacca atggcgctgt tcctccaaaa
     7861 atagccatgc ttactcccca tgcaacagaa acaccgctat atcgaatctg agtaggaaaa
     7921 aaatcattca atagtttaaa aattggtgtg ccgaccagag cattgcataa tgcataggga
     7981 atctgcgata agagaattaa tgtaaaagaa ttagtttgag ggatcaggaa agcaacagga
     8041 gccgcaataa aataccccca caaacctgtt tttataatat gtctttcgtc aattcgatca
     8101 gcatacattc ctgttaaaac aatgaatagt acaaccaatc cttgccctaa ggtaacaacg
     8161 aatttggttt gagcctctga caatgtagtg tattttgcca aatgagaagc ataataaaca
     8221 ttcataatgt agatataaat acctaatgca gctattaata atacagccct tattaattgt
     8281 tttttataat tttcaaataa aattttcagt ggattggatg atttttcttt acttagtgtg
     8341 aggtagggtg gtgattcact gagattttta cgaaaataca tcgcacaaaa gcaggataaa
     8401 gttcctaaca ggaaaggaat acgccatgcc cattcgggca tataaggtag ataaattaaa
     8461 gtagacatta atccgccaaa catcatgccc aatgcggaag caaatccagt ccagctaccg
     8521 gcttggcatg gtttttgtga atcatattca atcagataga ttgcgctacc attaaattca
     8581 ccagacacgg caaagccttg gacacaacgg aatatcaata aaagaattgg agccaagatg
     8641 ccaatactat cgtaagcagg aagacaacca atgcataatg aagataaccc catcagcata
     8701 atagaagatt gcagggcaac tcgacgtccc atgttgtctc caagatatcc aaaaaagagg
     8761 gctcctaaag gacgaaaaaa gtaactgagc gcgaaaacag aaaaggttgc tatcaaacca
     8821 agatgatttc ccaattttgg aaaaaacgat cccgagataa tatttgcgat ataggcgtaa
     8881 taagtaaact cagcccattc cagaaaagtt ccggcggttg tgacaacgac ccttttcttc
     8941 ttattttcca tacaataaat caatagaata ttaaagtgat gtagagagat tttaccttaa
     9001 attgatctca ttattctatg taattggatg atataacaga ttcataaagt tactatagtg
     9061 tcacattctt cttcta
//
LOCUS       DASLOE010000121         9055 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.122, whole genome shotgun sequence.
ACCESSION   DASLOE010000121 DASLOE010000000
VERSION     DASLOE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.122"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..398
                     /locus_tag="U6309_002178"
     CDS             <1..398
                     /locus_tag="U6309_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HEN5505616.1"
                     /translation="ALLDIIRSHTPVRYNELNIPLTITQKHAEAVLHVVHDVGGQPDG
                     PLDKSTHKPTLWEKNTHCTAECLAWRGHWVAEERRRRENDLGETIYFGTPYYARWLLA
                     MAKMLLDKGYITPDELMNKMAEVRKREEI"
     gene            399..707
                     /locus_tag="U6309_002179"
     CDS             399..707
                     /locus_tag="U6309_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HEN5505617.1"
                     /translation="MDKYKIGDRVTVSNLPSLFHTRTQGYTRGHTGEIVLLRPEWVIP
                     EDEAWGRYEGRRERFYLVRFKLNELWEDYGGSPIDTLETEFSERWLEPAKENSKKEKK
                     "
     gene            707..1375
                     /gene="scnC"
                     /locus_tag="U6309_002180"
     CDS             707..1375
                     /gene="scnC"
                     /locus_tag="U6309_002180"
                     /EC_number="3.5.5.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiocyanate hydrolase subunit gamma"
                     /protein_id="HEN5505618.1"
                     /translation="MKEEHLHEPIEEHNEAIEFNILETAIRELAIEKGLFSREDHRRF
                     TEWYDNLGYYSGSRVVARAWVDPEFKKRLLADGVAACKELGIDWLKPTGEGTPSDYTH
                     LIVCENTPKLHNTMVCTLCSCYPRPLLGIAPAWYERPNYRRRLVRWPRQVLAEFGTII
                     PPDVEVRVHDSNQRTRFMILPMRPEGTEDWSEEKLATIVTRDTMIGVAIPRIDWTVDS
                     IPGT"
     gene            1389..2015
                     /locus_tag="U6309_002181"
     CDS             1389..2015
                     /locus_tag="U6309_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEN5505619.1"
                     /translation="MFKMSLRTVFVAVIAILMPSVVFAHHAEFMSKNPFWQGFSMPIH
                     GIDHMLFSLAVGLIAAQIGGRALWSVPLVFSVAMLLGSILNVSGVAIPLLQYGILASL
                     VICAALLSWRTSVSLLLTVGLIGLFAIFQGNAMTTEYNLVYSMPFFIAGCLISSLILL
                     SIGVGLGLLISYLNKSIFRYLGVMMLAAAIVIGVFPDFDTSIVQLLNT"
     gene            complement(2026..2703)
                     /locus_tag="U6309_002182"
     CDS             complement(2026..2703)
                     /locus_tag="U6309_002182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505620.1"
                     /translation="MYYKNENYLTISPSMVYGLSNKVSFLVTVPVAASYRNETSRSNG
                     LGDTYFQGEFAFYEHSNFRYVDQMTFVGGLTAPTGSVNKDPSIGFGAVSYFLGGTYNR
                     MSVDWLAIASTGLNVITKHHGMKLGNQYLYQMGLGRNIKSKTGKYIFFGLVEVNGIYT
                     GKDKFIGEVDPNSGGNLVNIIPSLWFSTKKLFFQLGVALPIIQTLNGEQNKIHYNLVA
                     TAGITFN"
     gene            complement(3316..3879)
                     /locus_tag="U6309_002183"
     CDS             complement(3316..3879)
                     /locus_tag="U6309_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN5505621.1"
                     /translation="MVEDLMLCTNFFIFTGGPGSGKTAVLNELNTWGYLTVSEVARSI
                     IQKQQADGGDAIHTGNRKAFRDLMLEQSIADYCKMQSEEKIVFFDRGIPDLYGYSKAF
                     CGEASKKVNEAVRRFRYNKTAFIFPPWSEIYENDDERQQDYQEALQTYYAVKEGYFDC
                     GYVLLEVPKCPIKDRANFIFQMISKNR"
     gene            complement(3937..4896)
                     /locus_tag="U6309_002184"
     CDS             complement(3937..4896)
                     /locus_tag="U6309_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HEN5505622.1"
                     /translation="MIDDPIDLLRQYSRKLVRELGMLQLNKLAAKEQPSYWHTLIEIN
                     KEPNITISKLSQLLLVSLPTLSRIVNSLINDELVTANEGLDKRERFLRVTDKGKEKVN
                     YIDEYSNTKIKRAFHYLTDEEKEQVITAIGKYAQALEQSRLIRDQIRILRLSTSRALR
                     KQIVNMIENIQVNEFHLSITPEINASILKAEEEYCYYSSCNFWYAVDEQGGIIGSIGL
                     KKLNHTEGEVKKFFIAPKYRGLGLAQKLMLTLVRSAMKHGFKKLFLGTVAQLNAARRF
                     YEKNGFVLVNKNSLPKEFELCPLDTHFYYCETDKLDKSLRELI"
     gene            5186..5845
                     /locus_tag="U6309_002185"
     CDS             5186..5845
                     /locus_tag="U6309_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEN5505623.1"
                     /translation="MEQWHQFTLLFPLIILVFISLISPGPDFAITVKNSLAYSRRSGL
                     LTALGIALGSLVHISYTLLGLGLFITKTPIILHIIQYLGAGYLFYIGLKNLGAKPNAD
                     QIGSYATYNDLKPSEAFFNGFITNLLNPKAMLFFISLFSVLMPREISISNKLFLAIIL
                     FLQTLLWFSFVAFSLSGKNTRTKFQRVSYWIERITGVILIALALKLLLSQLGSVDISP
                     A"
     gene            complement(5969..6517)
                     /locus_tag="U6309_002186"
     CDS             complement(5969..6517)
                     /locus_tag="U6309_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505624.1"
                     /translation="MKYLTTPRLLLRAWDEEDTIPFYRMSQDPRVMKYFPGLWSMDMV
                     NDFIIRMNEQLSQKNFTLWAAEVKDCKQFIGFIGLNAPTWNAHFTPCVEIGWRLATEF
                     WGQGYATEGAKAALEYAFLEMHIPEIVSFTVPDNSRSRGVMDRLGMIRDIAGDFLHPK
                     LASDHRLAKHVLYRIQNPLIKV"
     gene            complement(6514..7303)
                     /locus_tag="U6309_002187"
                     /pseudo
     CDS             complement(6514..7303)
                     /locus_tag="U6309_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            7381..7800
                     /locus_tag="U6309_002188"
     CDS             7381..7800
                     /locus_tag="U6309_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN5505625.1"
                     /translation="MYLSSLLKKTNRLLIKRANELIKPYAMSHAYTYILMELYIEDGL
                     TQSELVQLIEVEQPTLVRTLDRMERDGFITRKPSSMDRRVVHIFLTEKARLLQEKIDE
                     CSRILNDEILAGFSEDEKTKLQELTHRVLDNLRNPIE"
     gene            complement(7830..8285)
                     /locus_tag="U6309_002189"
     CDS             complement(7830..8285)
                     /locus_tag="U6309_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEN5505626.1"
                     /translation="MPVKKLKQFLDSHKIKYLSIAHSPAYTAQEIAASAHVSGKQLAK
                     TVIIKMDGRLAMVVLPASDHITFMKLKEAIDASDLELATESEFEGKFAECDVGAMPPF
                     GNLYGLPVLVSTKLSAQDNILFNAGSHSELMQLSFGDFEKLVKPTLVTL"
     gene            complement(8445..9017)
                     /locus_tag="U6309_002190"
     CDS             complement(8445..9017)
                     /locus_tag="U6309_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEN5505627.1"
                     /translation="MLLPIQVTFRNMDPSKAVESRAIKLAKKLDCLYDKIMGCRVVIE
                     SPHRHHNKGKLYHVRIDLTVPDAELVVSRESSNKHAHEDVYVSLRDAFNAIERQLKNY
                     ANQRRGHVKRHEDSIRGGRVLEVCPIADYGYIETIDGRLIRFTGQSVVDYDINKLEVG
                     DRVRFVETVKNTQEAAASTVYVEGKRHIMN"
BASE COUNT         2859 a         1799 c         1567 g         2830 t
ORIGIN      
        1 ctgctctatt agatataatc agatcacata caccagttag atataatgaa ttgaatatac
       61 cgcttactat tacgcaaaaa catgcggaag ctgtattaca cgtagttcat gatgttggcg
      121 gccagccaga tggccctctc gataaatcaa cgcataagcc tactctatgg gaaaagaata
      181 ctcattgcac agcagaatgt ttggcttggc gcggtcattg ggttgcagaa gagcggcgaa
      241 gacgagaaaa cgatctgggt gaaaccatat attttggcac accttactac gcaagatggt
      301 tactcgcaat ggccaaaatg ttgcttgata aaggatatat cacaccggat gaattaatga
      361 acaaaatggc agaagttaga aagcgtgagg agatctagat ggataaatac aaaataggcg
      421 atcgtgtaac ggtcagtaat ttgccttctt tgtttcatac tagaacccaa ggttacacac
      481 gaggacatac aggagagatt gttcttttgc gcccagaatg ggttattccg gaagatgaag
      541 cgtgggggcg atatgaaggg cgtcgtgagc gattttacct ggttcgtttt aagttaaatg
      601 aattatggga agattacggc ggtagcccaa ttgatacctt agagacagaa ttttcagagc
      661 gttggcttga accagcaaaa gagaattcga aaaaggagaa aaagtaatga aggaggagca
      721 tttacacgag cccattgagg aacataatga agcgatagag tttaacattc ttgaaactgc
      781 tattcgtgaa cttgcaatcg aaaaaggctt gttttcacgt gaagatcacc gtcgctttac
      841 agaatggtat gataaccttg gatattattc aggttcaaga gttgtcgctc gcgcctgggt
      901 agatcctgaa ttcaagaaac gtttgctcgc tgatggggta gcagcatgta aagaactggg
      961 aatcgactgg ttaaaaccaa caggcgaagg tactcctagt gattataccc atttaattgt
     1021 ttgtgaaaac acacccaaac ttcataacac tatggtatgc actttatgct cttgctaccc
     1081 aagaccttta ttaggcatag ctccagcctg gtatgaaaga cctaattata gacgtcgttt
     1141 agtgagatgg ccacgtcaag tattagctga atttggtact attattcccc ctgatgtgga
     1201 agtacgagtt cacgactcca atcagcgcac acggtttatg attttaccta tgcgaccaga
     1261 gggtacagag gattggagtg aagaaaaact tgcaacgatt gtaaccagag atacaatgat
     1321 aggagtcgcc atcccaagaa ttgactggac agttgattca atacctggca cttaaaggga
     1381 gaaagctcat gtttaaaatg tccttgcgta cagtttttgt tgcagtaata gctatcttaa
     1441 tgccttctgt tgtatttgcc catcatgctg aatttatgag caaaaatccc ttttggcaag
     1501 gcttcagtat gccgatacac gggatagatc acatgttatt ttccttggca gtagggctaa
     1561 tcgccgcgca aattggaggt cgcgccttat ggagcgtacc tttagtcttt tctgtggcta
     1621 tgctattagg cagtatatta aatgtttccg gggtagcaat tccattactt caatatggta
     1681 ttttggcgtc tcttgtaatc tgcgcagctt tattatcatg gcgtaccagc gtatctttgt
     1741 tattgacggt tggacttatt ggtctttttg ctatattcca gggcaacgcg atgactaccg
     1801 aatataattt agtatatagc atgccatttt ttatagcagg ctgtttaatt tcttcattaa
     1861 ttctattaag cattggagtc ggcctcgggt tgcttatttc atatttaaat aaatctatat
     1921 tccgttattt gggtgtcatg atgctggctg ctgcgatcgt gattggagtt ttccctgact
     1981 ttgatacgtc tatcgttcaa ttattaaata cttaatatta aacatttaat taaatgttat
     2041 tcctgctgtc gcaacaagat tataatgaat tttattctgc tccccattga gagtttggat
     2101 aatggggaga gctactccta actgaaaaaa caatttttta gtcgaaaacc acaaggaagg
     2161 aataatattc acaagattgc cgcctgagtt tggatcaact tctcctatga acttgtcttt
     2221 tccagtatag attccattga cttcaaccag accaaaaaaa atatattttc cagttttact
     2281 tttaatattt cgacctagac ccatttgata taaatattga tttcccaatt tcatgccatg
     2341 gtgttttgtt atcacattta atccggtaga agcaatcgcc aaccaatcca ctgacattcg
     2401 attgtaagtg ccccctaaaa aatagctaac agcaccaaag ccgatagagg ggtctttatt
     2461 aactgaaccc gtcggagcag tcaagcctcc cacaaaagtc atttgatcaa catatctgaa
     2521 attcgaatgt tcatagaaag caaattctcc ttgaaagtat gtatccccca aaccatttga
     2581 acgtgatgtt tcattacgat agcttgcagc gacaggtact gtaaccaaaa aggaaacttt
     2641 attagataag ccatacacca tggaaggaga aatagtcaga taattttcat ttttataata
     2701 caaatagttt ggtgaaacgt aaaattgtat ctgattttta tcaatgagag attggccaaa
     2761 agaaaaaaag gaccaggttg ttgagaggtg ggaagagcaa aattacccag attcggaggc
     2821 tcattttgtg attctgagga agaagcttct ccaattgcga actttggcaa tgagctgata
     2881 attaaaagca ggcttaggct tcttatgtta catagaaaat ttttcataat gctcttttat
     2941 ttgtttgaac atattctata ctctcggcac cgtaaaactc tatcaattga taattggaga
     3001 tagttgttat taattaacat aaaattgggc cttttttagc gccaaattcc aacactattt
     3061 taataatcac cagtgtactg tttaagagca gtttcaggta aaaatttaca taaaactatg
     3121 atatttatta gtaatttcca aagataatat cacaaacgct aatggcaact ggcaatttat
     3181 attaaagcga gcattaaatc ttttgatcat tctataagga atagttgtat atgcattaag
     3241 taagttttcg cttttctcct tatgctacga acagactctc caaccaggat aactggaacg
     3301 aatacctcga caggctcatc tatttttact tatcatttga aaaataaaat tagcccggtc
     3361 tttaataggg catttgggaa cctctaataa cacataccca caatcaaaat acccctcttt
     3421 tacagcataa taagtttgca acgcttcttg ataatcctgt tggcgctcat cgtcattttc
     3481 ataaatttca gaccagggag ggaaaataaa ggctgtttta ttataacgaa atcgcctaac
     3541 agcctcattc actttcttac tcgcctcacc acaaaatgct ttggagtaac catacaaatc
     3601 cgggattccc cgatcgaaaa atacaatttt ttcctctgac tgcatcttgc aataatctgc
     3661 aatcgattgt tcaagcatca agtctcgaaa cgcttttcta tttccagtat gtatcgcatc
     3721 accaccatca gcctgttgtt tttgaataat agaacgtgct acttcagaaa ctgttaaata
     3781 gccccaagta tttaactcat tcaatacggc agttttgcca gagccaggtc ctcccgtaaa
     3841 tataaaaaaa ttagtgcata gcattaaatc ctctaccata acctgtcata cctttaaaaa
     3901 ttgctcataa attaaattac aaagctttta gtgtactcat atcaattctc ttaagctttt
     3961 atccagctta tctgtttcgc aataataaaa atgggtatcc aaagggcata gctcaaattc
     4021 tttaggtaaa gaattcttat taacaagaac aaatccattc ttttcataaa aacgtcttgc
     4081 tgcgtttaac tgggctactg ttcccaaaaa cagcttttta aatccatgtt tcatggcgct
     4141 tctaactaaa gtcagcatta atttttgagc taatcctaac cctcgatact tcggcgcaat
     4201 aaaaaatttt tttacttccc cttcagtatg atttaacttc tttaaaccga tagaaccgat
     4261 aatccccccc tgttcgtcaa ccgcatacca aaaattacaa ctgctataat agcaatactc
     4321 ctcttcggcc tttaaaattg atgcattaat ttcaggagta atcgaaagat gaaactcatt
     4381 cacttgaata ttttcaatca tattaacaat ttgtttacgc aaagctctgg aagttgacaa
     4441 gcgtaaaatt ctaatctgat cacgaattaa acgactttgt tctaaagctt gagcatactt
     4501 tccaattgca gtaattactt gttccttctc ttcgtcagtt aaataatgaa atgctctttt
     4561 tattttagta ttagaatatt catcaatata atttaccttt tccttaccct tatcagtgac
     4621 tcttaaaaag cgctcccgct tatccagccc ttcgtttgcc gtaaccaatt catcattaat
     4681 caaagaattc actatccgcg acagggtagg taatgaaact aacaacaatt gactcaattt
     4741 ggaaatagta atattgggtt ctttatttat ttcaattaaa gtatgccaat aagaaggctg
     4801 ctcctttgca gctaacttat ttaattgcaa catacctaat tctcgtacca attttcttga
     4861 atattgtctg agcaaatcaa ttggatcatc aatcatctcg ccactctcac tataaaaatt
     4921 atattaataa ttgcatatat tataaattaa cattaaaata attccatatt ttatgtattt
     4981 ttattaaata tacatggttt gaaagcgaga aaatcccttt tatgaggata ggaaaccacc
     5041 ctttccacca cctgtggtgg gtacatttag ggagttaaaa taagataaac aatgtcttat
     5101 taactaaatg taaaattaaa aaagagcgct taaaaattag ctatgatatt ggctgaaaaa
     5161 acttagtaaa aaacttggag tctctatgga acaatggcat caatttactc tactattccc
     5221 tttaattatt ttagttttta tttcgttaat tagtcctgga ccagattttg ctatcacagt
     5281 caaaaacagc ttggcttatt ccagaaggag tggtttatta accgcgttag gaattgcttt
     5341 gggcagtttg gttcatatta gttacactct ccttggatta ggcctgttta ttaccaaaac
     5401 acctataata ctgcatatca tccaatatct aggggctgga tacctctttt atattggcct
     5461 taaaaatctt ggtgccaagc caaatgctga ccaaatagga agttatgcaa cgtacaacga
     5521 tttaaaacca agtgaagctt ttttcaatgg gtttataacg aatttattga accccaaagc
     5581 catgctcttt tttatcagtt tgttttcagt acttatgccc agagaaattt caatctccaa
     5641 taaattattc ctagcaatta tcctttttct acaaacattg ttatggtttt cttttgttgc
     5701 attcagtctt tctggaaaaa atacgcgtac gaaatttcaa agagttagct attggattga
     5761 acgtattaca ggtgtcattt taattgcatt ggcattaaag ttactattaa gccaattagg
     5821 gtctgttgat atttcgcccg cttgagtcga aaagccttga ttttcgagca ccgtagcgaa
     5881 gcacagaaaa tcaaggcttt tcggccaaga tagtagaaat gtcaacagac cttagtctaa
     5941 tggccggata aaacatatac aaaatccatc aaacttttat taaaggattt tgtatacgat
     6001 ataaaacatg ctttgctaac ctgtgatcag aggctaattt gggatgtaaa aagtctcctg
     6061 caatatctct gatcatccca agtctatcca taacacctct tgagcgggaa ttatcaggca
     6121 cagtaaaaga aactatctcg ggaatatgca tctccagaaa agcatattca agagctgctt
     6181 ttgccccttc tgtagcatag ccttgccccc aaaactcagt tgccaaacgc catcctatct
     6241 ctacgcaagg agtgaagtga gcattccaag ttggagcatt taatcctata aaaccaataa
     6301 actgcttaca gtcctttact tcagccgccc acaaagtaaa gtttttttga gataattgtt
     6361 cattcattct gataatgaaa tcattgacca tatccataga ccacaacccg gggaaatact
     6421 tcatgacccg aggatcctga ctcattctat agaaggggat agtatcttct tcatcccacg
     6481 ctcttagcaa aagccttggg gttgttaaat atttcatggc aaatatattc ttccttccaa
     6541 ataaagatgg caatttccat agagcaatac tctgttttcc ttcacttcac aataaagatc
     6601 accttgtctg aggcttcctt gtaaagcagt tatatggttc ttccttaact gttcacacca
     6661 ataaggagca ataacacaat gagcagatcc tgttaccggg tcttctggca cattacatcc
     6721 aggataaaaa caacgagaat aaacatcagt tgatattcct ggagcagtta atattaaccc
     6781 cctatagtca agatgagcca cagcattcaa atcaggcttt gcctcaacta cttcttctgc
     6841 attttgatac acaaacatca aatcaaattg gcttcgcaat gctttaaatg gcctttttaa
     6901 attcaaatgc tgcagttcgg cactgaaatt aatttcttca tagggcagaa ctgggaaatc
     6961 cataaccaag ccttgatctg tgtttttaac ccatagctta ccactcaaac tattaaatat
     7021 aatttcttcc cctgaaaagc ccaattgatt aaaaagtaca taagagctgg caagagtggc
     7081 atggccgcac aatgccactt cttcattggg ggtaaaccac cggatcctgt atccatcggt
     7141 ttcgggagca aaaaaggctg tttcagataa attgttttca gctgcaatgg cttgcatagt
     7201 ctcatcactt aaccatttat ccaaatggac aaacagcggc aggattacct ttgaataaat
     7261 ttgaggcaaa tgcatccact tggtagattg gtaagctttt cataaatgtc tcctaaaaca
     7321 acttgcgaat aataacatag cctgttatta tatagcaagc tatttaataa tgagaaattg
     7381 atgtatcttt cctctctgct aaaaaaaaca aatcgtttgt taatcaagcg agccaatgaa
     7441 ttaattaaac cctatgctat gagtcatgcc tacacttata tattaatgga actgtatatt
     7501 gaagatggtt tgacacaatc tgaattagtt caattgattg aagtagagca gcctaccctt
     7561 gtaagaactt tggatcgaat ggaaagagac gggtttatca ctcgaaaacc aagctcaatg
     7621 gatagaagag tcgtgcatat ttttcttaca gaaaaagccc gacttttgca agaaaaaata
     7681 gatgaatgct ccagaatact taacgacgag attctggctg gtttttcaga agatgaaaaa
     7741 acaaaattac aagagctaac tcatcgcgtt cttgataatt taaggaatcc cattgaataa
     7801 tttataatga tatttctcat cgcttcattc tatagcgtca ccaatgttgg ctttactaac
     7861 ttttcaaaat caccaaaaga gagctgcatc aattcagagt gagaacccgc attgaataag
     7921 atattatcct gtgcactcag tttagtcgac accaacactg gtaaaccata caaattaccg
     7981 aatgggggca tagctccaac atcacattcc gcaaatttcc cttcaaactc tgattcagta
     8041 gcaagttcaa gatcagaagc atcaatagct tctttaagtt tcataaatgt aatatggtca
     8101 cttgcaggca atactaccat agctaaacgt ccatccattt ttataataac tgtctttgcc
     8161 agttgttttc ctgaaacatg agcagaagca gcgatttctt gtgcagtgta cgccggtgaa
     8221 tgagctatag acaaatattt tatcttatgg ctgtccaaaa attgtttaag ctttttaact
     8281 ggcatgacaa actccttttc gatactaata cattctttca gaaaatgctc tctctcaagt
     8341 gcccttattt aaaattttga atcatttgtt tggaaacagt catttcgcct aaacaattac
     8401 tcaacagcaa actgcaaatt accaataggc ctctgatacc cctgttaatt cataatatgg
     8461 cgtttacctt caacgtaaac cgtacttgca gctgcctcct gtgtgttttt aaccgtttca
     8521 acaaatcgta ctcggtcacc aacctctaat ttattgatgt cataatcaac gacactttgg
     8581 cctgtaaatc gtatcagacg cccatcaatt gtttctatgt agccataatc agcaatggga
     8641 catacttcaa gaactctccc accacgaata gaatcctcat gacgcttgac atggcctcgc
     8701 cgttgatttg cataattttt aagctggcgc tctatcgcat taaaagcgtc tcttagagaa
     8761 acatatacat cttcatgcgc atgcttgttt gatgattcac ggctcacaac caactcagca
     8821 tcaggaactg tcagatcaat acgtacatga taaagctttc ctttgttatg atggcgatga
     8881 ggagactcaa tgactactcg acaacccatt attttgtcat aaagacaatc aagcttttta
     8941 gcaagtttta tagccctgct ttcaacagct ttagatggat ccatatttcg gaatgtcacc
     9001 tgaataggaa gtaacatttt taacctccct tatgttttga ttatgaataa tttca
//
LOCUS       DASLOE010000122         9028 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.123, whole genome shotgun sequence.
ACCESSION   DASLOE010000122 DASLOE010000000
VERSION     DASLOE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.123"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            163..432
                     /locus_tag="U6309_002191"
     CDS             163..432
                     /locus_tag="U6309_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505628.1"
                     /translation="MINYKLSHWGCCLIVLFALGVSGCYFDGVSPQERGYSYQENNMP
                     PSTGGQTTASNQSQPQQKSYASKDTSQKSTPGPKRTAAPQLPVIQ"
     gene            complement(815..2209)
                     /gene="fumC"
                     /locus_tag="U6309_002192"
     CDS             complement(815..2209)
                     /gene="fumC"
                     /locus_tag="U6309_002192"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEN5505629.1"
                     /translation="MAKTRVETDSMGEIEVPSDKYWGAQTERSLHHFNIGKDIMPREV
                     THAFGILKKAAALTNLELGKLPKDKADLIVQAAEEVSKGLLDEHFPLHVWQTGSGTQS
                     NMNANEVISNRAIELAGGTMGSKSPIHPNDHVNMSQSSNDTFPTAMHIAAALAFNGKL
                     LPAVRNLRNVFAAKMDAFKDIIKIGRTHLQDAVPLTLGQEFSGYVAQLDACIHRLEEV
                     LPELYELAAGGTAVGTGLNTHPQFAVKVAEHIAKITKLPFVTAPNKFAALASHEPLVH
                     AHGAMKTLACALMKIANDIRWLASGPRCGIGELIIPENEPGSSIMPGKVNPTQCEAMT
                     MVCAQVLGNDTAVGIADSQGNFELNVFKPVIIFNVLHSLNLLADSCHSFQEFCAEGIE
                     PNRPVIDYYLHHSLMLVTALNQHIGYDKAAKIAKTAHHDNISLQEAAVKLGILTAEQF
                     AQFVKPEKMISPEQ"
     gene            2372..3106
                     /locus_tag="U6309_002193"
     CDS             2372..3106
                     /locus_tag="U6309_002193"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEN5505630.1"
                     /translation="MAVRTIRIYQPGEYQPEQLLELSPEAGQHVGVVLRMEQGEQLTL
                     FNGDNREFAASIEKVKKKQVFVRIASVLEVNRESPLKIHLAQAISRGERMEMVMQKSV
                     ELGVACITPLITERCQVKIDKEKMAKKMHQWLNIVIGACEQCGRNQIPELRQPVYLEQ
                     FVREAKEHLRFILHPAFNKTWRDYPVQPPDVALIIGPEGGLSDEEITLTSDYGFLPLS
                     LGPRVLRTETAAITALSVLQAVGGDL"
     gene            3422..3748
                     /gene="trxA"
                     /locus_tag="U6309_002194"
     CDS             3422..3748
                     /gene="trxA"
                     /locus_tag="U6309_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEN5505631.1"
                     /translation="MSDHIKTVTDASFDQDVINSSKPVLVDFWAEWCGPCRALTPILE
                     EVAAAHSKDMTFAKINIDENPQAPAKFGVMSIPTLILFKNGQVEAVKMGLLSKSQLSA
                     FVESHL"
     gene            4070..5332
                     /gene="rho"
                     /locus_tag="U6309_002195"
     CDS             4070..5332
                     /gene="rho"
                     /locus_tag="U6309_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEN5505632.1"
                     /translation="MNLSELKQLPIADLVNIAQEMGVENTSRMRKQDIIFAILKAHAL
                     KGEDIHGDGVLEVLTDGFGFLRSADGSYLAGPDDIYVSPSQIRRFGLRSGDTISGKIR
                     PPKDSERYFALLKVDQINYDTPDSAKRKILFENLTPLFATERLVMEQGNGSTEDLTAR
                     VVDLCAPFGRGQRGLIVSPPKAGKTLMLQNIARSIEKNYPECYLIVLLIDERPEEVTE
                     MQRSVKGEVVASTFDEPANRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRLYGAARNIEEGGSLTIIATALVDTGSKMDEVIYEE
                     FKGTGNMEIHLSRNIAERRVFPAININRSGTRREDLLLSPEELQRTWILRKILQSMDE
                     CDAIEFLLERMKNHKTNAEFFDAMKRQE"
     gene            5477..6943
                     /gene="ubiD"
                     /locus_tag="U6309_002196"
     CDS             5477..6943
                     /gene="ubiD"
                     /locus_tag="U6309_002196"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEN5505633.1"
                     /translation="MKYSDLRDFIAQLESRELLKRIDYPVSPHLEMTVVSDKVLRSGG
                     PALLFTNTPNYDMPVLTNLFGTVERVALGMGEESIVALREIGKLLAALKEPDPPKGFK
                     DAFSKLPLLKQALNMAPKYVSGAECQTHVWEKDEVDLTLLPIQTCWPGDVAPLITWGL
                     VTTRGPHQSRENMGIYRQQLLSKNKLIMRWLSHRGGALDYQAWQQEYPKERFPVAVTL
                     GADPATILAAVTPVPDTLSEYAFAGLLRGQRTRLTRCIGNDLHVPASAEIVLEGYLEP
                     GNEAPEGPYGDHTGYYNEVQSFPVFTVERITHRDKPIYHSTYTGRPPDEPAILGVALN
                     EVFIPLLQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYPGHAKRIMMAVWSFLRQFMYT
                     KFVIVCDDDVDARNWQDVIWAMTTRMDPSRDTVMVENTPIDYLDFASPVSGLGSKMGM
                     DATSKWPGETQREWGKPITMDEDVLNRVNGYWSLLGLK"
     gene            6943..7656
                     /locus_tag="U6309_002197"
     CDS             6943..7656
                     /locus_tag="U6309_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HEN5505634.1"
                     /translation="MTDKIIQAQVEDISPLTDSIVRLVLMPDEYVDYQAGQYLQILFA
                     NDAFSYSIANAPLGSHRYELHIRHSQDNDYHQSLLAHIKQYGTLRLRLPLGECSIDQL
                     HPQKPILFIAGGTGFAPIKAMIEQLLADSDSRPFELFWGARSQSDLYMDDKVRSWRSH
                     ASHFQYFSLLSDKSEDTLVSRVLARHANILRDWQIVISGPFDMVYSTRDTLVGYGISP
                     TQMFSDAFSFESKTDDAKN"
     gene            7675..7869
                     /locus_tag="U6309_002198"
     CDS             7675..7869
                     /locus_tag="U6309_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505635.1"
                     /translation="METLYQILGLIGAGLIIFILYRFIKGSPGQFSKENMSKSFMTMG
                     VLGLILIGFIALLVLMLRNT"
     gene            complement(7907..8218)
                     /gene="hspQ"
                     /locus_tag="U6309_002199"
     CDS             complement(7907..8218)
                     /gene="hspQ"
                     /locus_tag="U6309_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEN5505636.1"
                     /translation="MNKIAKYNIGDLVIHKRIRYRAIVIDVDPIFQASGKYNPRATQH
                     EFATRNPWYRLLVDDSSQITYVEECQLLPDTSQEPINNPNIDDYLIKNKGSYQKTIPS
                     H"
     gene            complement(8280..8996)
                     /gene="tatC"
                     /locus_tag="U6309_002200"
     CDS             complement(8280..8996)
                     /gene="tatC"
                     /locus_tag="U6309_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEN5505637.1"
                     /translation="MLIHLIELRQRAIYTLLWFGALALLFFFISDQLFHLLVRPLLRS
                     LPNQEGLIATQITSSVFTPLKLAVDTALLLTAPFALLQLWRFMRPGLYKNEQEQIRGT
                     IILSLLLFVFGTLFCFYLVLPYMFQFFAQALPEGVRLMPDMAYAIDFITRMLLLFGFS
                     FQVPLICLVLVKTHFTTVETLIKIRPYVIVGAFVIGMLLTPPDVFSQVALALPLCLLY
                     EAGIILARIYGKSPVPTLIQ"
BASE COUNT         2611 a         1733 c         2024 g         2660 t
ORIGIN      
        1 attaatcggg atggcttaat taatttaaac ttaacttaaa ttaagttgaa tcgttctatt
       61 tttatatcag tggttatttg tgaattttta cattatattt attcactgga agttgattta
      121 gtttaaattt tggttctatt taattactgc aggaagctta ttatgatcaa ttacaaatta
      181 tcacactggg gatgttgcct catagtactt tttgctttag gggtatcagg atgttatttt
      241 gatggggtaa gccctcaaga aagagggtat tcttatcaag aaaataacat gccgccatcc
      301 actggaggac aaaccaccgc tagcaatcaa tcccagccac aacaaaaaag ctatgcctca
      361 aaggatacct ctcaaaaatc tacccctggg cctaagcgaa cagcagcccc tcaactcccg
      421 gtaattcaat aaaaggagtt ttacttatta tccctcataa tcaccttata tgagggataa
      481 ctgaaacttg ttcataagat tccatcacta tgaaatatca taatgcttgt agttgatgga
      541 ttatcaaaga agaaggatag aagcctatag tgcagatatt cgactcatgc aaaagagcga
      601 gatttacatt ttatctattt atttgtgctg agcttaaaaa tcttaataag ataatcagga
      661 tttaaggagt gcctgtaaac aaggcgaatc atataaaaaa tcagattcat tccggcgaat
      721 gtaattcaaa ctattgtttg ttgtcatttt gttttctact acatcttagg ttaagacatg
      781 ccagaaccac cggcatgtct taaccctctc aatttcattg ctcaggggaa atcatctttt
      841 ccggcttcac aaactgagca aattgctcgg cggttaaaat tcccagtttc acggcggctt
      901 cttgcaatga aatattgtca tgatgagcgg ttttggcgat tttagccgct ttatcataac
      961 cgatgtgctg gttaagagcg gtaaccagca tgagggaatg gtgtaaatag taatcaatta
     1021 ctggccgatt aggctctatt ccttctgcgc aaaattcctg gaaggagtgg cagctgtcgg
     1081 caagcaggtt taatgagtgc aaaacgttaa atatgatgac cggtttaaac acgtttaatt
     1141 caaaattgcc ctggctgtcc gcaattccaa cggctgtatc attacccagt acctgggcgc
     1201 aaaccatggt catggcttcg cattgtgtcg ggttgacttt tcctggcatg atggatgatc
     1261 ctggctcatt ttctggaata atcagctcac caatcccgca acgaggtcct gaggcgagcc
     1321 agcgtatgtc gttggcgatt ttcattaatg cgcaagcgag tgttttcatg gcgccgtgcg
     1381 catgcaccag aggttcgtga gaagccagag cggcaaattt gttaggggca gtcacaaaag
     1441 gcagtttggt gattttagcg atgtgttcag cgactttgac tgcgaattgc ggatgggtgt
     1501 tcaatccggt tccaacggca gtgcctccgg cagccaactc gtataattca ggcaatacct
     1561 cttccaggcg gtgaatgcag gcatccagtt gagcgacata acctgaaaat tcttgaccca
     1621 aagtcaatgg aaccgcatct tgtaaatggg ttctgccgat ttttatgatg tctttgaaag
     1681 cgtccatttt tgctgcgaaa acattgcgta aattgcgcac agcgggtaat aattttccat
     1741 taaaagccaa ggccgcggca atatgcatgg ctgttgggaa agtgtcattc gatgattgag
     1801 acatattgac gtgatcattg ggatgaatag gggatttgct tcccatagta ccgccagcca
     1861 gttcaattgc gcgattggaa atgacttcat tggcgttcat attggattgt gtgccgcttc
     1921 ccgtttgcca gacatgcaag ggaaaatgct catccaacag gcctttgctc acttcttctg
     1981 ctgcctgaac aatcaaatcc gctttgtctt ttggcaattt gcctaattca agattggtca
     2041 gggcggctgc ctttttcagg ataccaaagg catgggttac ttcgcgtggc ataatgtctt
     2101 tgcctatatt gaagtgatgc aatgaacgtt cggtttgggc gccccaatat ttatcagacg
     2161 ggacttcaat ttcacccatg ctgtctgttt ctacacgtgt tttagccatg ttatcaattc
     2221 cttagcaaag ttaatctgtt gattacgttt atttatgtct agctgcttta tcagcgagtt
     2281 gggaaagaat aaaatcaatc gccaaaattc tagcatagca gacgtaaatt ggtaattttt
     2341 gttatagtgt cattatcgaa atgaggaata attggctgtg agaacaatac gtatctatca
     2401 acctggtgaa taccagccag agcaacttct ggaattatcg cctgaggcgg ggcagcatgt
     2461 tggggttgtc ttgcgtatgg agcaaggcga acaactgact ctgtttaatg gggacaatcg
     2521 agagtttgca gcatccattg agaaggtcaa gaaaaagcag gtattcgtta gaattgcctc
     2581 agtccttgag gtgaacaggg aatccccatt aaagatacat ttagcacaag ccatttccag
     2641 gggcgaacgg atggaaatgg tgatgcaaaa atcggttgag ttaggagtgg catgcattac
     2701 acctcttatt accgagcgct gccaggttaa aattgataaa gaaaaaatgg caaaaaaaat
     2761 gcatcaatgg cttaatattg tcattggcgc ttgtgagcaa tgtgggcgta atcaaattcc
     2821 agagttgcga cagccagttt atttagagca atttgtcaga gaagccaaag agcatctcag
     2881 gttcattctg catccggcat ttaacaagac atggcgcgat tatcctgtcc agccccctga
     2941 tgttgccttg attattggtc ctgaaggggg attaagcgat gaggaaatta cattaaccag
     3001 tgactatgga tttttgcctt tatcactggg accgagagta ttgcgcactg aaaccgcggc
     3061 aataactgcg ttaagtgttt tgcaagctgt cggaggtgat ctataataga cttctttaca
     3121 aattctgctg cggtaggaaa ttgtttgcgg acatggtgtt cgaatcctga tgtactgacg
     3181 tcaattccct cacttaagct aatttggaaa gaagtctgat aagccgctgt ataacctgcg
     3241 tcttttgttt tatagctcta ttgcaagtat ttctgtagta ttatatgtaa actactcttt
     3301 ttgaaaatct tgtgtttgct ctaaaacaaa atgcacagga tgtgcaagat agattccgga
     3361 tcgaatagca acatctggtc ttggatcata aatttatatt tttaataact gaggtttatt
     3421 tatgagtgat catattaaaa cagtaaccga tgcaagtttt gatcaggatg taattaattc
     3481 atccaagccc gttctggttg atttttgggc tgaatggtgt gggccatgtc gtgcattaac
     3541 tcctattttg gaagaagtgg cagctgctca tagtaaggat atgacgtttg ctaaaatcaa
     3601 catcgatgaa aacccccaag ctccggccaa atttggtgtg atgagtatcc caaccttaat
     3661 tctttttaaa aatggccagg ttgaagcagt taaaatgggt ttgctgtcta aatcccaatt
     3721 aagtgctttt gttgaaagcc acctgtaatt gtataaaaat taacaagccc tgagatgaaa
     3781 atttatttac agggctttta tcttttttat gaagtatgtg ttaaaattaa gctatgaaag
     3841 ggattttttc ttaaagattt aaaagttaaa atcaaaaagg tcaaaataac tttttatttt
     3901 tagtttaaaa tgttggatct tgttgtaaag ctgtgatagc ctgtagctat atgtgacata
     3961 ctttaaaaca agtgagccac ataggcagcg gattccttag ctgctattcc ctaacataat
     4021 tttttcttcg gatattcaat tcatttaaac aacaaatcaa gtgagaagta tgaatcttag
     4081 tgaacttaag caattaccta ttgccgatct cgttaatatc gctcaagaga tgggtgtgga
     4141 aaatacctcc cgtatgcgca agcaagacat cattttcgcc atccttaaag cccatgcttt
     4201 aaaaggggaa gacatccatg gcgatggtgt tttggaagtt ttgactgatg gttttggttt
     4261 cttgcgttcc gctgatggct cctatctggc aggccctgat gacatttacg tctctcccag
     4321 tcaaatcaga cgtttcggtc ttcgttcggg ggatactatt tctggtaaaa tccgcccccc
     4381 caaagacagc gaacgctatt ttgctttatt aaaagttgat caaattaact acgatacacc
     4441 ggatagcgca aagcgtaaga ttttatttga aaacttaact cccttgtttg ctaccgagcg
     4501 tctggttatg gagcaaggta atggcagcac agaagatctc acagcaagag tagttgattt
     4561 atgcgctcct ttcggccgcg gacagcgtgg tttgatcgtg tcacctccga aagcaggtaa
     4621 gacattaatg ttgcaaaaca ttgctcgctc tatagaaaaa aattatcctg aatgttatct
     4681 cattgttttg ctgattgatg aacgtcctga agaagtaacg gaaatgcagc gttctgtcaa
     4741 aggggaagtt gttgcgagta cttttgacga gcccgccaat cgccatgtcc aggttgccga
     4801 aatggtaata gaaaaagcaa aacgtctggt tgagcataaa cgtgatgtgg ttattctgct
     4861 cgattcaatc actcgcctgg ccagagctta caatacagtt gtaccagcgt caggtaaagt
     4921 actgactggt ggtgttgatg cgaatgccct gcaaagaccc aagagattgt atggtgcggc
     4981 acgtaacata gaagagggtg gcagtttaac cattattgca acggctctgg tagatacggg
     5041 ttccaaaatg gacgaagtga tttacgaaga gtttaaaggt acaggtaaca tggaaatcca
     5101 cttgagtcgt aatattgccg agagaagggt tttccctgcg attaacatta atcgctctgg
     5161 tactcgccgt gaagatctcc tgttgagccc cgaagaatta cagcgcacct ggatattgcg
     5221 taaaatcctg caatccatgg atgaatgcga tgcgattgaa ttcttattgg aacgcatgaa
     5281 gaatcataag acaaatgccg aattctttga tgcaatgaag cgtcaggaat aatttaaatg
     5341 ataattaacc ccgtaattag cggagcataa ttcggggttc aaattcgcct tattttcagg
     5401 tttataattc atgctcatag cctgtattac gctgtgctgg tatgggttta cagtaagctt
     5461 ctttttgaga taattaatga agtattcaga tctgagagat ttcatagccc aacttgaatc
     5521 acgtgaatta ttaaaacgta ttgattatcc tgtatcacct catcttgaga tgaccgtagt
     5581 cagcgataaa gtgttgcgct caggagggcc agcccttctg tttaccaata cccccaatta
     5641 cgacatgcct gtactgacca atctttttgg tacggtagag cgcgtggctt tgggaatggg
     5701 tgaggaatca atagtggctt tgagggagat tggaaaatta ttggctgctt taaaggaacc
     5761 cgatcctccc aaaggcttca aagacgcttt tagcaagttg cctttattga aacaagcgct
     5821 gaatatggca cccaaatatg ttagtggagc cgagtgccag actcatgtgt gggaaaagga
     5881 tgaagtggat ttaaccttat tacccatcca aacgtgttgg cccggagatg ttgctcctct
     5941 aattacctgg ggtttggtta ctactcgtgg cccacaccag tccagagaaa acatgggcat
     6001 ctatcgccag caactattaa gtaaaaacaa attgatcatg cgctggttat ctcaccgcgg
     6061 aggtgctttg gattaccagg cctggcaaca agaatatccc aaagaacgtt tccctgttgc
     6121 ggtgacttta ggcgctgatc cagccaccat actggcagca gttactcccg ttcctgatac
     6181 tttgtctgaa tacgcttttg cgggcttgct tagaggacaa cgaactcggt tgactcgatg
     6241 tattggcaat gatttgcatg ttccagccag cgcagaaatt gttttggaag gttatctgga
     6301 gccaggaaat gaggcgcccg aagggcctta tggcgatcac accggttatt ataatgaagt
     6361 ccaatctttt cctgttttta cggtagagcg tattactcat cgcgataaac ctatttacca
     6421 cagtacttat accggaagac cgccagatga gccagccatt ttgggggttg ccttaaatga
     6481 agtgttcatt cccttgttgc aaaaacaatt cccagagatt gtggattttt atttgccgcc
     6541 agagggatgc tcttatcgtt tggctgtagt tactataaaa aagcaatatc caggacatgc
     6601 taaacgtatt atgatggctg tttggtcttt cttgcgccag tttatgtata ccaagttcgt
     6661 cattgtttgt gatgatgatg tggacgcgcg caattggcaa gatgtcatat gggcaatgac
     6721 gacacgcatg gatccgtccc gcgatacagt catggtagaa aatacaccca ttgattatct
     6781 ggattttgct tccccagttt caggattggg ttccaagatg ggtatggatg ctaccagtaa
     6841 atggccagga gaaacacaaa gagaatgggg taaaccaatt acgatggatg aagatgtgct
     6901 taatagagta aatggttatt ggtccttatt aggattaaaa taatgacaga taaaataatt
     6961 caagctcagg tggaggacat ttccccttta acggacagca tcgtgcgatt ggttttaatg
     7021 cctgatgaat acgttgatta tcaagccgga cagtatttgc aaatcctgtt tgcaaatgat
     7081 gcatttagtt attcaattgc aaatgcgcct ttaggttctc atcgttatga attgcatatt
     7141 cgccatagcc aggataatga ttatcatcaa tcgttattgg ctcatatcaa acaatatgga
     7201 actctgcgct taagattacc tttgggtgaa tgcagtatcg atcaattaca tccccaaaaa
     7261 cctattttat tcattgccgg cggaactgga tttgcaccaa ttaaagccat gattgagcaa
     7321 ttactggcag attcggacag caggccattt gagttatttt ggggagcacg ctcacaaagc
     7381 gatttataca tggatgataa agtaaggagt tggcgctccc atgccagcca cttccaatat
     7441 ttttcgctat tatccgataa aagtgaggat actttggttt ctcgggttct tgccaggcac
     7501 gcaaacattc tacgggactg gcaaattgtc atcagtggtc ctttcgatat ggtgtacagt
     7561 actcgtgata ccttggttgg ttatggcata tcgcctaccc agatgttttc agatgccttt
     7621 agttttgaat ccaaaacaga tgatgcaaaa aactgagtgg aatggagtga attaatggaa
     7681 actttatatc aaattttagg actcattggc gccggattga ttatttttat attgtaccga
     7741 tttattaagg gaagcccagg gcaatttagc aaggaaaata tgagcaaaag ctttatgacg
     7801 atgggggttt taggcctgat tttaataggt tttattgcct tgctggtgtt gatgttgagg
     7861 aatacataac aagctccatg ccctgttagc atctcagcct cataagctag tgagacggga
     7921 tagttttctg ataactgccc ttgtttttaa ttaaataatc atcaatattg gggttgttga
     7981 taggttcctg actcgtatca ggaagtaact ggcattcttc gacataagtt atttggctgc
     8041 tgtcatcaac taacaggcgg taccatgggt ttcttgttgc gaattcatgc tgagttgccc
     8101 ggggattgta tttccctgaa gcctggaata tgggatccac atcaataaca atcgcccggt
     8161 atctgatgcg tttatgaata accagatcac cgatattgta tttggctatt ttattcataa
     8221 aaggctccat ttttgagcat tatgataaat atatcggtaa ctccgctttt aacttgagct
     8281 tactgaatta aagtgggtac gggggatttc ccataaatcc tggctaaaat aatccccgcc
     8341 tcataaagca gacacaaagg caatgctaat gcaacttgag aaaaaacatc agggggggtg
     8401 agtagcatac caataacaaa tgccccaact atcacataag gcctgatttt tataagtgtc
     8461 tcaaccgtag tgaaatgggt ttttaccagt acaagacaaa ttagtggaac ctgaaaagaa
     8521 aaaccaaaca aaagcagcat gcgtgtaatg aaatctatgg cgtaagccat atcaggcatg
     8581 agtcgcaccc cttccggaag ggcctgagca aaaaactgga acatataagg gaggacaaga
     8641 taaaagcaaa atagtgtccc aaagacaaag agcaaaaggc ttaaaatgat ggtgcctctg
     8701 atttgttcct gctcgttttt ataaagtccc ggacgcataa aacgccaaag ttgcaataaa
     8761 gcaaacggag cggtgagcaa caaagcagta tctactgcaa gttttaaagg ggtgaaaacc
     8821 gaggaagtaa tctgtgtggc aatcaagccc tcttgattcg gtaaagatcg caataacggc
     8881 cttaccagga gatgaaacag ttggtctgat atgaaaaaaa acaatagcgc taaagcgcca
     8941 aaccagagga gggtatagat tgctctttgc cgtaattcaa ttaaatgaat taacataaaa
     9001 tcagttttga ttaaatataa aaatatag
//
LOCUS       DASLOE010000123         8929 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.124, whole genome shotgun sequence.
ACCESSION   DASLOE010000123 DASLOE010000000
VERSION     DASLOE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8929
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.124"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..234
                     /locus_tag="U6309_002201"
     CDS             <1..234
                     /locus_tag="U6309_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HEN5505638.1"
                     /translation="LFAKQLNEINQQLISIETQLQELENIRHNDGSYQHALRILEMGG
                     TREEIISSCHLSNAEAELLMNLQAYRTALKHQN"
     gene            294..1401
                     /gene="prfB"
                     /locus_tag="U6309_002202"
     CDS             join(294..368,370..1401)
                     /gene="prfB"
                     /locus_tag="U6309_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114835330.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEN5505639.1"
                     /translation="MLEVNQINLSLIDLNERIESLRGYLDFEGKSERLEEVIRELESS
                     NVWDNPEQAQALGRERTQLETIVHSLSQLSQSISDQRELFELAREEDDEQTINDIGEE
                     LKSIEQQVASLEFRRMFSGKMDHSNAYLDIQAGSGGTEAQDWAEMLLRMYLRWGEHHG
                     FTTELIECSPGEVAGIKSATIHFTGEYAFGWLRTETGVHRLVRKSPFDSGNRRHTSFA
                     AVFVSPEIDDDIEIEINPADLRIDTYRASGAGGQHVNRTDSAVRITHIPSGIVVQCQN
                     DRSQHKNKDQAMKQLRAKLYELEMQKKNAEQQALEASKSDIGWGSQIRSYVLDQSRIK
                     DLRTGVETSNTQAVLDGSLDQFIEASLKAGVGQA"
     gene            1398..2888
                     /gene="lysS"
                     /locus_tag="U6309_002203"
     CDS             1398..2888
                     /gene="lysS"
                     /locus_tag="U6309_002203"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEN5505640.1"
                     /translation="MSEEHVHLDESEVYHIRKQKLAELRTSGFNFPNTFRREHLADAL
                     LKQYSETEKQTLEQKHVKVSVAGRIVLRRIMGKASFFHIQDVSGRIQVYLRSNDLPDV
                     YEQFKHWDLGDIVGVQGELFKTNTGELTINAEHVELLTKSLRPLPDKFHGLADQELKY
                     RKRYVDLIANEDSRKTFLIRSHLIKAFREFMDDNHFLEVETPMMHPIPGGALARPFVT
                     HHNTLDMTMYLRIAPELYLKRLVVGGFERVYEINRNFRNEGISTRHNPEFTMLEFYQA
                     YADYNDLMNFTEQLFHYLCDKVLATRQIEYQGQVIDFNKPFERLSVKEAILKYHPDTK
                     AQQLETVEGCRTLLNDLGLPYKETDGLGKLQIILFEETVEHQLFQPTFITEYPTEISP
                     LARRSDTNPEVTDRFEFFIAGREIANGFSELNDAEDQAERFRKQVEEKDAGDLEAMHF
                     DSDYIEALEYGLPPTAGEGIGIDRLVMLFTNSQSIRDVILFPHLRQ"
     gene            complement(2975..3460)
                     /locus_tag="U6309_002204"
     CDS             complement(2975..3460)
                     /locus_tag="U6309_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1776 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505641.1"
                     /translation="MQSKIHKKEDNTNYSFLLKCMAAATAALATAAIVTAVVIKATAT
                     TAVGFGVATAGAALSPLLVVGGALLATVGICCLPLLLCCGSSRSVYTVSNSPAYRPWS
                     SAWYTGGYNPTFHSHPVYNNGGTYHTHHSGTVHTHPNPVHTHGNNVHVHTTPSNVHGH
                     R"
     gene            complement(3774..4877)
                     /locus_tag="U6309_002205"
     CDS             complement(3774..4877)
                     /locus_tag="U6309_002205"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEN5505642.1"
                     /translation="MNYNDTQEIVVTPLMGATGLETLEMGAARIHVDDKQIINCRADL
                     NQLVPFKYKWAWDKYLTACANHWMPNEISMSADVALWKDPYGLTEDERLIIKRNLGFF
                     STADSLVANNLVLAVYRHITNPECRQYLLRQAFEEALHTHAYQYIIESLGLDEAEVFN
                     MYREIPSVSRKAVWALPFTQSLGDETFHTGTPENDQRLLRDLIAFYVVFEGIFFYVGF
                     TQILSMGRRNKMVGTSEQFQYILRDESMHMNFGIDVINQIKIENPHLWTPEFKNEMIQ
                     LIREGVELEYQYARDTMPHGILGMNAEMFEEYLHFIANRRLNQIGLPDQYPGAENPFP
                     WMSEMLDLKKEKNFFETRVIEYQAGGTLSWDDE"
     gene            complement(4891..7719)
                     /locus_tag="U6309_002206"
     CDS             complement(4891..7719)
                     /locus_tag="U6309_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEN5505643.1"
                     /translation="MSEILEPVKDIPLTNQLELTANVPGILKTIKRNGKVVIYDDTKI
                     KIAITKAFIADEGGTAPTSDRIHQQIEELTRQITQVFKRRLPSGGAIHIEEIQDQVEL
                     ALMRSGHYKVARSYVLYREERRKARESESKQKQTKDSKHLLITMPNGEVQPLDMDRVN
                     TIVKESCRDLSNVKAEPVIKDALRNLYNQAKYEDVHKALIMSARALVEKEPNYTYVSA
                     RLLLDSLRSEALTKLNIKSEATFDEMAQIYPVYFKSYIEQGIAQGMLDPKMADFDLDK
                     LSQALLPERDMKFTYLSLQTLYDRYFIHERGVRYELPQAFFMRVAMGLALREQHKEEK
                     AIEFYQLLSSFDYMSSTPTLFNSGTVRPQLSSCYLTTVPDHLDGIYSAIKDNALLSKY
                     AGGLGNDWTPVRAMGAHIKGTNGKSQGVVPFLNVADATAVAVNQGGKRKGAVCAYLEC
                     WHRDVEEFLELRKNTGDDRRRTHDMNTALWVPDLFMKRVREDGNWTLFSPDEVPDLHD
                     QYGKAFEALYVEYEEKARQGIIRNTKTISAVKLWRRMLSMLFETGHPWLTFKDPCNLR
                     SPQQHAGVIHSSNLCTEITLNTSEEEIAVCNLGSINLPAHIKNGKLDEQKLKRTISTA
                     VRMLDNVIDINYYSVPQARNSNLQHRPVGLGIMGFQDALYELKMDYASKEAIEFADSS
                     MELISYYAIEASCDLAKERGSYSTYEGSLWSKGILPIDSINLLQQSRNKYLEQDRSQK
                     LDWEPLRVKVRTQGMRNSNVMAIAPTATISNICGVSQSIEPTYQNLYVKSNLSGEFTV
                     VNPYLVADLKALNLWDEVMVNDLKYFNGSVQPISRIPDELKRRYATSFEIDPMWLVEA
                     ASRRQKWIDQAQSLNIYMAQPSGKKLDQLYMHAWIRGLKTTYYLRSLGASNAEKSTVT
                     DGALNAVKLIDTEPKACSITDPDCEACQ"
     gene            complement(7991..>8929)
                     /locus_tag="U6309_002207"
     CDS             complement(7991..>8929)
                     /locus_tag="U6309_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEN5505644.1"
                     /translation="EIRTIIYNYGEEEILKHILYESKEPILIKIPDFTSQFSLDYFEQ
                     LTQAKTTYCTFENKRCVDFQAGNFAEVIQQIKSNKPIRIFGQILSRERSKEIEKHVPL
                     WQKIPFRPRYFRETKKVAYFFGGKGAVSEMHFDREHCCNLHLCLSGKKQVLLFTRKQS
                     DNLYKLPFIGDSLIDFTQPLDVLVQQYHRLNQAEGYQVLLEKGDMLFMPRNCWHYTEY
                     LDASSAATYVFYPKKFFQYYGYFTGNFFLGYKEESGFKIYRWPFFEKFSRRYALSTGV
                     SKITFKIIEKILFLFMLPIISLLNIISHKFNPRRVF"
BASE COUNT         2686 a         1617 c         1831 g         2795 t
ORIGIN      
        1 ttatttgcga aacaacttaa tgaaattaat cagcaattaa ttagtattga gactcaattg
       61 caagaattgg aaaacataag acataacgat ggcagttatc aacatgcctt gagaatcctg
      121 gaaatgggtg gcactcgtga ggaaattatc agtagttgtc atttatcaaa tgctgaagca
      181 gagttactaa tgaatcttca ggcgtataga accgcattaa aacatcagaa ttagtataga
      241 tagaatctta ctttcgctgt aaaatctcct atttattttt aaaagagaca ctgatgctag
      301 aagtgaatca aattaattta agtttaatcg atcttaatga gcgcatagaa tcgcttaggg
      361 ggtatctttg acttcgaagg taaaagtgag cgcttggaag aagtaattcg cgaattggaa
      421 tcatccaatg tgtgggacaa tccggaacag gctcaggcat tgggacgtga aagaactcag
      481 ctggaaacta tagtccactc attaagccaa ttaagccaat caattagtga tcagagggaa
      541 ctatttgaat tggctcgaga agaagatgat gagcaaacca tcaacgatat tggtgaggaa
      601 ttaaaatcaa ttgagcaaca agtcgccagt ttggaattta gaagaatgtt ttctggcaaa
      661 atggaccact ccaatgctta tcttgatatt caagcaggtt ctggtggcac tgaagcgcaa
      721 gattgggctg aaatgctatt acgaatgtat ttacgttggg gagaacatca cggatttact
      781 accgagttga tagaatgctc tcctggtgaa gtggcaggaa ttaaaagtgc gactattcat
      841 tttacaggtg aatatgcgtt tggttggtta aggactgaaa caggtgtcca tcgtttagtg
      901 agaaagtcgc catttgattc tggcaacagg agacacactt cgtttgcagc tgtttttgtc
      961 tctcctgaaa ttgacgatga tatcgaaatt gaaattaatc cagctgattt gcggattgat
     1021 acttacagag cttccggggc tggcgggcag catgttaacc gtactgattc tgccgtaagg
     1081 attacacata tccctagtgg gatagttgtt cagtgtcaga atgatcgaag ccagcataaa
     1141 aacaaagacc aggcaatgaa gcaattgagg gcgaagcttt atgagttgga aatgcaaaag
     1201 aaaaatgcag agcagcaagc attggaagct agcaaatctg acattggttg gggatcacaa
     1261 attcgttctt acgtattaga ccaatcccgt ataaaagatt tgcgcaccgg tgttgaaacc
     1321 agtaatactc aggcagtatt ggatggttca ttagatcaat ttattgaagc cagtttaaag
     1381 gcaggagtag ggcaagcatg agtgaagaac atgtacattt agatgaaagt gaagtttatc
     1441 atattcgcaa acaaaaatta gcggaattgc gcacaagcgg gtttaatttt ccaaatacct
     1501 ttcgccgcga acatttagcc gatgctttgc tgaagcaata ctccgaaact gagaaacaaa
     1561 cattagaaca aaagcatgtg aaagtatctg ttgcaggccg aatcgttctg agaagaatta
     1621 tgggtaaagc cagttttttt catattcaag atgtatcagg tcgaatacaa gtttatcttc
     1681 gttcaaatga tcttcctgac gtttatgaac agtttaaaca ttgggattta ggagatattg
     1741 ttggtgtaca gggtgagttg tttaaaacaa acactggtga actgaccatt aatgcagaac
     1801 atgttgagtt attaactaaa tctttaaggc cgcttcctga taaattccat ggtttggctg
     1861 atcaggaatt gaaatatcgt aaacgttatg ttgacttgat tgcaaatgaa gacagtcgaa
     1921 aaactttttt aatacgttca catttaatca aagccttcag agaatttatg gatgataatc
     1981 attttctgga agtcgaaaca ccaatgatgc atccaattcc tggaggtgct ttggctcgtc
     2041 ctttcgtaac tcatcacaat acattggata tgaccatgta tttgaggata gcccccgaat
     2101 tgtacttaaa acgattggtt gttggtggtt ttgagagagt atatgaaatt aatagaaatt
     2161 tccgtaacga aggaatttct actcgtcata atccagagtt cacgatgctc gaattttacc
     2221 aggcttatgc cgattacaat gatttgatga atttcactga acaattgttt cattatctat
     2281 gtgataaagt attagctact cggcaaatag aatatcaagg ccaggtcatt gatttcaata
     2341 aaccttttga acggttgagc gtaaaagaag ctatattaaa atatcatcct gatactaagg
     2401 cgcagcagtt ggaaacggta gagggatgca gaacacttct gaatgattta ggtctgcctt
     2461 ataaagaaac tgatggtctg ggtaaactac aaattattct ttttgaggag acagtagagc
     2521 accaattatt tcagcccact tttattacgg aataccctac agaaatatct cctttagcga
     2581 gacgttctga tacaaatccg gaagtcaccg atcgatttga attttttatc gctggtcgtg
     2641 agattgcgaa tggattttct gaattaaacg atgccgagga tcaagctgag cgctttcgaa
     2701 agcaagttga agaaaaggat gcaggcgatt tagaggcaat gcattttgat agtgattata
     2761 ttgaagcatt ggaatatggc ttaccgccta cagctggtga agggattgga atcgatcgct
     2821 tggtgatgtt atttactaac tcgcaatcaa tcagagatgt tattttattt ccgcatttaa
     2881 gacagtaaag tacagcaatt tttagatagc aatggtttat ttagaaccat tgctatgtca
     2941 agcaatacat aaaatacact tggtttagat aaaattatct atgtccatga acattacttg
     3001 gagtagtatg tacgtgaaca ttattaccat gtgtatgtac cggattagga tgggtatgta
     3061 cggttcctga gtgatgagtg tggtaagtac cgccattgtt ataaacagga tgcgaatgaa
     3121 aagttggatt atagcctcct gtataccatg cactgctcca aggcctgtaa gcaggtgagt
     3181 ttgacacggt atatacacta cggctgctgc cacagcagag caataatggg aggcagcaga
     3241 tacccacagt ggctagtaat gctcctccta caactaataa tggcgataga gcagctcctg
     3301 ctgttgcaac cccaaaacct acggccgttg tagcagtggc tttgattacc acagcggtta
     3361 ctattgctgc tgtagcaagt gctgctgtag cagcagccat acattttaat agaaagctgt
     3421 aatttgtatt gtcttctttt ttgtgtattt tagactgcat aacttacctc atcttgattt
     3481 tcagttaata gaatgcattt ttttgggtgt gttatatctt tatagtgttg ttattatttt
     3541 gcgggcatag cattatttct atgcgcttgg gattttacct gatcatacta aaatgtcacc
     3601 ataaatttgg atttggaggt tgatttgttc aaagttaatt caaaaaataa tggaaatatg
     3661 tagaatatta ttgagtttga ttaagttaaa gactgggtta tgtccttttt cacaaagtaa
     3721 gggatttctc taaatgaact cccttacttt gctcgaggta acatgattaa actttattca
     3781 tcatcccagc ttaaagtacc gccagcctga tattcaataa cacgagtttc aaaaaagttt
     3841 ttctcttttt tgagatccag catttcactc atccagggga atgggttttc agcacctgga
     3901 tattgatccg gcagacctat ttgatttaaa cgacggtttg caataaagtg caaatattct
     3961 tcaaacatct cggcgttcat tcctagaatg ccatgaggca tagtgtctct ggcgtattgg
     4021 tattcgagct caacaccttc tctgatcagt tggatcattt cattcttaaa ttcaggtgtc
     4081 cataaatgag gattttcgat tttgatttga ttaatcacat caataccgaa attcatatgc
     4141 atggactcat cgcgcagtat atattggaac tgttctgatg ttcctaccat tttgttacga
     4201 cgtcccatag agagaatttg agtaaatccg acataaaaga aaataccctc aaacacgaca
     4261 taaaatgcaa ttaaatcgcg tagcaggcgt tgatcatttt ctggagtgcc tgtatggaag
     4321 gtctcatcac ctaaactttg agtaaatggt aatgcccaaa ccgcttttct ggacacagaa
     4381 ggtatttctc tatacatatt aaatacttca gcttcatcca ggcccaaact ctctataatg
     4441 tattgataag catgagtgtg taacgcttct tcaaaagctt gccttagaag atattgacga
     4501 cactcaggat tagtaatatg tctgtaaaca gcaagaacca gattattcgc aacgagtgaa
     4561 tcagcggttg agaaaaatcc caaattacgc ttaatgatta aacgctcatc ttctgtaaga
     4621 ccataaggat ctttccataa ggcgacatcg gcactcatac tgatttcatt gggcatccaa
     4681 tgattagcac aggctgtcaa gtatttatcc caagcccatt tgtatttgaa aggcaccaat
     4741 tgatttaagt cagcacggca attaatgatt tgcttatcat cgacatgaat gcgtgcagcc
     4801 cccatttcta gagtttcgag ccctgtcgcg cccataagag gagttacgac aatttcttga
     4861 gtatcattgt agttcataaa tttctcctga ttattggcaa gcctcgcagt ccggatcagt
     4921 aatagaacaa gcctttggtt cggtatctat taatttgact gcgttaagtg ccccgtcggt
     4981 aacagtggat ttttcagcat tacttgcacc taaactacgt aagtagtaag tggtttttaa
     5041 tccacgaatc catgcatgca tataaagctg atccagtttt ttaccagaag gttgggccat
     5101 atagatgttc aaggattggg cttgatctat ccatttttga cggcgtgacg cggcttcaac
     5161 caaccacatg gggtcaattt caaaagaagt ggcataacgt cttttcagct catctggaat
     5221 tcgactgata ggttgtacac taccattaaa atattttaga tcattgacca tgacttcatc
     5281 ccaaagattc agagctttta aatcggccac caaataggga ttcaccacgg taaattcgcc
     5341 tgacaaattg gatttgacgt aaaggttctg ataagttggc tcaatagatt gtgatacacc
     5401 gcagatgttg gatattgtgg ctgtaggtgc aatagccatt acatttgagt tacgcatgcc
     5461 ttgtgtgcga actttaactc gtaatggttc ccaatccagt ttttgtgacc tgtcttgctc
     5521 cagatattta ttacgggatt gttgcaagag gttaattgaa tcgataggta atatgccctt
     5581 gctccataga gaaccctcat aggttgagta actgccccgt tctttagcca ggtcacaaga
     5641 ggcttcgata gcatagtaac tgattagttc catggaagag tcagcaaatt caatggcttc
     5701 tttggatgca taatccattt ttaattcgta tagcgcatcc tgaaagccca tgatacctag
     5761 accaactggc cgatgttgca agttggagtt acgagcttga ggtacagaat aataattaat
     5821 atcaatgaca ttatccaaca tacggacagc tgttgatatt gtgcgcttga gtttttgctc
     5881 atccagttta ccgtttttaa tatgcgctgg aagattgata ctgcctaaat tgcacacagc
     5941 aatttcttct tcagaagtat ttagagtaat ctctgtacat aaattagaac tgtgtattac
     6001 tccagcatgt tgttgaggtg atcgtaaatt gcagggatct ttgaatgtta gccatggatg
     6061 accagtttca aaaagcatgg aaagcattct acgccatagt ttgacagctg aaatggtttt
     6121 tgtattccta ataatacctt gtcgagcttt ttcttcatat tcaacataga gagcctcaaa
     6181 tgctttacca tattgatcat gtaaatcggg tacttcatca ggtgaaaata aagtccaatt
     6241 accatcttca cgaacacgct tcataaacaa atcaggtacc caaagggcag tattcatgtc
     6301 gtgagtacgg cggcgatcat caccggtatt ttttcttaac tccagaaatt cctctacatc
     6361 tctgtgccag cattccaggt acgcgcagac agctcccttg cgttttcccc cctgatttac
     6421 tgctacagca gtagcatcgg caacattcag gaaaggaact acaccttgag atttaccatt
     6481 agttcctttg atatgagcac ccatagctcg tactggagtc cagtcattgc ctagtcctcc
     6541 agcatattta gagagaaggg cattgtcttt tatagcgcta taaataccat ccaaatgatc
     6601 gggtactgtt gttaaatagc aactggataa ctgcggcctt actgttccgg aattaaataa
     6661 tgttggagtg gaagacatat aatcaaatga agagagtaac tgataaaact ctatggcttt
     6721 ctcttcctta tgttgttcac gaagagcaag acccatggct actcgcatga aaaaagcttg
     6781 aggaagctca tatcttaccc cgcgctcatg aataaaataa cgatcatata aagtttgtaa
     6841 acttaaatag gtgaacttca tatctcgttc gggaagaaga gcttggctaa gtttgtccag
     6901 gtcgaagtca gccatcttgg ggtcaagcat accttgggca ataccttgct ctatgtaaga
     6961 tttgaaataa actggataga tttgagccat ttcatcaaat gtagcttctg atttgatatt
     7021 gagtttagtc agtgcttcag aacgtaagct gtctagcaac aaacgcgcgc tgacataagt
     7081 gtaattaggt tctttttcaa ccaacgcacg cgctgacata atcaaagctt tgtggacatc
     7141 ttcatatttg gcttggttat aaagattacg caaggcatct ttaataacag gttctgcttt
     7201 gacgttgctg aggtcacgac atgattcttt gacgatagta ttgaccctgt ccatatctaa
     7261 tggttgtact tctccattag gcatagttat taataaatgc ttactgtctt ttgtttgttt
     7321 ttgtttcgat tctgattcgc gtgcttttcg acgttcttct ctatataaaa cataggagcg
     7381 agctacttta taatgtccgc tgcgcataag agcgagttca acttgatcct ggatctcttc
     7441 aatatgaata gcgccaccac taggtaaacg gcgtttaaat acctgggtga tttgtctggt
     7501 tagctcttcg atttgctggt gaattcgatc agaagttggg gctgtaccac cttcgtcggc
     7561 aataaaggct ttggtaatag caattttgat tttagtatca tcataaatga ctaccttgcc
     7621 atttcgtttt attgttttaa gtatgcctgg gacattagca gtcaattcca gttgatttgt
     7681 tagcggtata tcctttaccg gctcaagaat ttcggacatt tttcctccac gaaatatatt
     7741 ttggcaattc attgtttcag tgaatccaac atgttttacg aacttgttta aaaggcaatc
     7801 ctagtttcgt tgaaactatg acttgctcct gtttttacga ccgtcaagag aagcttttgt
     7861 ttactgttac cataattata gcaaaaaacc caatatatag ggtattttag gggttttaat
     7921 aacaagataa tgtgtttttt tgctttggtc aatagaataa tctgggatta ttctgtggat
     7981 aagttgtggg ttaaaatact ctgcgggggt taaatttatg agaaataatg tttaataggc
     8041 taattatagg cagcatgaat agaaataaaa ttttttctat tattttaaac gtgattttgg
     8101 agactcctgt tgataatgca tagcgacggc tgaatttctc aaaaaaaggc catctataaa
     8161 ttttaaaacc ggattcttct ttatacccta aaaaaaaatt gccggtaaaa tatccataat
     8221 attggaaaaa ttttttagga taaaacacat aagtagcggc agatgacgca tcaagatact
     8281 ctgtgtaatg ccaacaattt ctaggcatga ataacatatc gcctttctca agtagaacct
     8341 gatatccttc agcctgattc agtctgtggt attgttgaac taaaacatcc aggggttgag
     8401 tgaagtcaat taaagaatcc ccaataaagg ggagtttgta aaggttgtca ctttgttttc
     8461 tggtaaacag caatacttgt tttttcccac tcaaacataa atgaagattg cagcaatgtt
     8521 ctcggtcaaa atgcatttct gagacagcgc ctttacctcc aaaaaaataa gccacttttt
     8581 tggtttctct aaaatacctt ggcctgaaag gaattttttg ccaaagtggc acatgctttt
     8641 ctatttcctt agagcgctct ctagacaaaa tttgcccaaa aatacgaatg ggtttgttgc
     8701 tttttatttg ttggatgact tcagcaaaat tgcccgcttg gaaatcaaca catcgcttgt
     8761 tttcaaaggt acaataagtg gttttggcct gagtgagttg ttcaaaataa tcgagtgaaa
     8821 attggctggt aaaatcaggt attttaatta agataggttc tttggattca tacaagatat
     8881 gcttaaggat ttcttcctca ccatagttgt aaatgattgt tctgatttc
//
LOCUS       DASLOE010000124         8677 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.125, whole genome shotgun sequence.
ACCESSION   DASLOE010000124 DASLOE010000000
VERSION     DASLOE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.125"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..395
                     /locus_tag="U6309_002208"
     CDS             <1..395
                     /locus_tag="U6309_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEN5505645.1"
                     /translation="LHLITLQSKEKDLRNRACFDAANAMSQLVGKLTTYTSYLEKNPD
                     PFLIKRYKECCIAEIDLANLTLQNHRDALWLVAELSTAILLLGIGYLFAFGINYYYTG
                     RIGLFSQTRSEKLVEEMKSSVLGVAVGN"
     gene            complement(449..1258)
                     /locus_tag="U6309_002209"
     CDS             complement(449..1258)
                     /locus_tag="U6309_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1484 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505646.1"
                     /translation="MPSREMNCCIGKSLNYMRFSMSSFKHSCEKIDINLAHNVLTICP
                     KNREALQRITALFNNLYILYFIGGIPSITYDDMDQIQINIKYSQQITDAKLKNTLLSF
                     VNSELISQDEKETFLNELELTKTSFTTQFNTELENIRKKAEELSKKAEVDPYHYESAS
                     KAASKLHQELLIASQLYFYHKTPEAYENFKQTCQREIERARPVLEQHRGWKPLLVNIG
                     AAIIGLGVLYLLAASINYYKTEGRHFFFHFETDSLQKLENLNIAQSQMLRV"
     gene            complement(1456..1857)
                     /locus_tag="U6309_002210"
     CDS             complement(1456..1857)
                     /locus_tag="U6309_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505647.1"
                     /translation="MQESIHSLFLAQAIGLYLLIVGIIMLSRASYYRELLTHLKEGSA
                     TIVTAGSLGLIIGICLVLVHNIWVPESEVLVTLVAWLILIKSILWLGFPEFMVKCSQK
                     AYTGTGYYVISIVVLIIGILLITHGFYLFSH"
     gene            complement(1943..2464)
                     /locus_tag="U6309_002211"
     CDS             complement(1943..2464)
                     /locus_tag="U6309_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEN5505648.1"
                     /translation="MKNKPTKNYPNESNTGYPVEILDKIDRKILNILQKNNQITNQAL
                     ADIVGISAPPCFRRVKRLREEKIIVNDVALVDPFKVGRPLIVFVNITLEKQREDLLAH
                     FERKMQEEPEVMQCYFVSGDTDYLLIIHVKDMNHYNEFARRVFANEPNIRKFRSSFCL
                     NRTKYNTQVVLDE"
     gene            2584..3114
                     /locus_tag="U6309_002212"
     CDS             2584..3114
                     /locus_tag="U6309_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505649.1"
                     /translation="MITIRLAEMNEIQFLNQLISCSARELSQEDYTKEEIEGAIQYVF
                     GVDLELILDKTYFVIEKDGQIAGCGGWSRRKTLFGGSQYKGREQGVYLDPQQDFAKIR
                     AFFIHPKFARQGLGSSLLKHCEHEAFLQKFTRFEMMATLPGVKLYSAFGYHPTSNEVI
                     TLPNNVPLRFVRMTKG"
     gene            3366..5939
                     /gene="lgt3"
                     /locus_tag="U6309_002213"
                     /gene_synonym="legC5"
     CDS             3366..5939
                     /gene="lgt3"
                     /locus_tag="U6309_002213"
                     /gene_synonym="legC5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector
                     Lgt3/LegC5"
                     /protein_id="HEN5505650.1"
                     /translation="MKEQQKAIFIENIKSTCAVDEQMAKELADSLLEIHQLNTGVNEQ
                     TLVNEVFLTKFALYLKIGKEVALDWVRKNKDLDTPFPSDTVKSQPVQDAYKPAENKVQ
                     VYGPMTIGKSPVKTTLNQLTGNLLVDVVEAGKKEKAQKLRAYIFDQLARRLEASLSQE
                     QINDLYNRIRGTGNYTKSESFSEEQLKILKEKIVPELKRELSDLGNGNVNILGLDVSR
                     EDKYAFDTTNIFSVWFSNNPAVYMPQHVKTQVEKTAKLNQPGKTRIVFSSLCLNETAQ
                     IDFQQWAKENNIELVDIDSIDLKSVSETDAQLLNLAKDELEAMRKGKGGNPAAASDLV
                     RWVDVIIGESSTYIDIDLPMNDKKVTVEVHSGFPVLLNMGSALTKDGQQPAMENPAFN
                     TDMIAYSKDKEARRQIIEGVAKKIIARYENCAKYIEESKNEELVRLKNTPGYQFLVKK
                     TDGKFDLCTLRAAVSEAHQDALSFATFFGAEYFAKTFATQELVPVIKEAIQHQNQDLL
                     TSVIENHIEKQHLNDYPKTPDGIKKLLKSFQGIVYKPLVMEFSGPSAVSSSWVEAISG
                     RSIPRNFEYLAEPMSQPLRVLQHHACVSGKANFSSDNIPKWCELQSDVEKRQLQREDG
                     LSWLPDAQEKLKREGQQAKLEEEQRIKLEEEQRIKLEEEQRIKLEEEQRIKLEEEQRI
                     KLEEEQRIKLEEEQRIKLEEEQRIKLEEEHKSKKYFAQSDAIDLILNNFENEINSIGA
                     YYAPAKQRAIELLDTLRKYKEEAFNDPSREKLISFAQNTKRAIQEATPILQKDLGWGD
                     YLANLAKQLVNAVTFAVAYAVTFGTTGHQGFFALKSSLAVSQSQNLDEELNSKLSQNN
                     C"
     gene            6149..7174
                     /locus_tag="U6309_002214"
     CDS             6149..7174
                     /locus_tag="U6309_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505651.1"
                     /translation="MRRSKAMQIPKKRTQILTISESSKEDLDVVSEYSDELDVSPNKE
                     TSKTNSKKLESTARKEQIKKLEGELYSHIQDAIELEEESFAEFSINTGRPSNYLFRFV
                     SEKEYLAILENLNQGKRIFGNKKKVFEEEDEKISTKAKGETFFAFDLKYAQKYFNKGT
                     RRDGVMLAIELLEEADTKLLSNSEIVRRIGDDPGMAGGRWAKCKKVKPGERGIVVVKC
                     EGVGSCKYPYTLGFREGEEGVSPFDVLFELTRSVKVLNKNGSCKTIYHYSVPTPKEKE
                     LINIVEDAENGIDEIHSDEFLSVVSSSPSSRFGFFTNKELSGISGLVNKAPEHIIPPS
                     DNTFSHH"
     gene            7340..8665
                     /locus_tag="U6309_002215"
     CDS             7340..8665
                     /locus_tag="U6309_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEN5505652.1"
                     /translation="MSALYYNLNNHYVSHVIHSPEKRANRDNNPEKIMKKPLDTTEQN
                     RIAALYELKILDTPADERFDRIIRLTSGYLSVPIAFISFIDSKHQWFKSSCGIGLNKI
                     SRNDSFCHYTIQQSKPMIIPDALLDERFCNNPYVTNSPNIRFYAGFPLSTVDGFNVGT
                     LCAIDKKPRNLDETEIQILKDLATLAEDQVNLLDISLLERAVREKNISLTKAKKELEM
                     RNNFIRKVFGSFMSDEIVAALLESKSELKLGGEERKITILFSDLRNFTTLSEHFPADK
                     IVAALNNHYGKMVDVIEKYNGTVDSFIGDAIMVVFGAPHSGGDDAYRAIACALEMQAT
                     MQEVNAMNRNSSLPELAMGIGINTGYAIVGNIGSQKRMQYSAIGSPVNLASRIQDLTL
                     GGQILISEATYQEANHSLELNGHLRVKVKGIAAPITIYDVVGLDKKSDS"
BASE COUNT         2890 a         1414 c         1723 g         2650 t
ORIGIN      
        1 acctgcattt gattacccta cagagcaaag aaaaagattt gcgtaataga gcatgttttg
       61 atgcagcaaa tgcaatgagc caattggttg gtaagctcac tacttacacc agttatcttg
      121 aaaaaaatcc agatccattt ttaataaaac gttataaaga gtgctgcata gcagaaatag
      181 atttagccaa tttgactttg caaaatcacc gagatgcctt atggttagtg gctgagttat
      241 caacggctat ccttttgctt ggtatagggt atttatttgc ttttgggata aattattact
      301 acacaggtcg tatagggtta ttttctcaaa ccaggtctga gaaattggtg gaggaaatga
      361 aaagctcggt attgggtgtt gcggttggaa attaaaaatc tgtatttccc ggattatagc
      421 agagccgtct aatccgggtt gttcatggtt agactcttaa catttgagat tgagctatat
      481 tcaggttttc taatttttgc aaagagtccg tttcaaaatg aaagaaaaaa tgacggccct
      541 ctgttttata atagttaatg gaagctgcaa gcaaatagag aactccaaga cctatgatgg
      601 ccgctcctat attaactaac aaaggtttcc atcctctatg ctgctccagt acaggtcgag
      661 ccctctcaat ttctctttga catgtttgct tgaaattttc gtatgcctcg ggtgttttat
      721 gataaaaata caattgggag gctattaata attcttgatg cagtttgcta gctgcttttg
      781 acgcggactc ataatgataa gggtcaactt ctgctttttt gcttaactct tcagcctttt
      841 ttctgatatt ttccaattcg gtattaaatt gggtagtgaa tgaagttttg gtgagctcca
      901 gctcatttaa aaatgtttct ttttcatctt gagagattaa ttcagaatta acaaaagaaa
      961 gtaatgtatt tttgagtttg gcatcggtta tttgttgaga atacttaata ttaatttgaa
     1021 tctgatccat gtcatcatag gtgatgctag ggatacctcc tataaaataa agaatgtaca
     1081 gattattgaa aagggcagta attctttgca gtgcctctct atttttagga caaatggtta
     1141 gaacattatg agctagattg atatcaattt tttcacaaga atgtttaaac gatgacatac
     1201 taaacctcat ataattcaga gatttcccta tgcaacaatt catttccctt gaaggcaggt
     1261 caagtatatg tagacaagct taagatttta ttaagaagcg ccttaaattt aacaattttt
     1321 caaaaaaact ttacgatttt ttgaaatgca aaaaatatag ttaacacttg cagatttctt
     1381 ttataaaaac ctacacactt catccattga tggttacctt gctgaaagca gagttgccta
     1441 tgaaagcagg taaaatcaat gggaaaacag gtaaaatccg tgggttatta acagaatccc
     1501 aataattaat accactatgg aaatcacata ataacctgtc cctgtatagg ctttttgact
     1561 gcatttcacc ataaactcgg gaaatccaag ccataatatt gatttaatca aaataagcca
     1621 ggctaccagt gtaactaata cctctgattc aggaacccaa atgttatgga ccagtacaag
     1681 gcaaataccg ataattaacc ccaagcttcc agccgttaca atagtggcac tgccttcttt
     1741 gagatgagtc aacaattcac gataatatga agctcgagaa agcattataa ttcccacaat
     1801 aagaagatag aggccgattg cttgtgccaa aaataaagag tgaattgatt cttgcatggt
     1861 tggctccttg tgattccgct cctaaattaa ttatagtaca tgcgggattg ggtttgttaa
     1921 ccaggcaggt acttgctagt tactactcat ctaaaacaac ttgtgtatta tatttggttc
     1981 tattaagaca aaagctgctt ctgaattttc taatattggg ctcattagcg aatactcgcc
     2041 tggcaaattc attgtaatga ttcatatctt tgacatgaat aataagcagg taatctgtgt
     2101 ctcccgagac aaaatagcat tgcataacct caggctcttc ctgcattttt ctttcaaaat
     2161 gagctagcag atcttctctt tgtttctcaa gagtaatatt gacgaacaca attaatggtc
     2221 tgcctacttt aaatggatca accaaagcaa catcgttaac aataattttt tcctctctca
     2281 ggcgtttgac tcttcgaaag cagggtgggg cagagattcc aactatatct gccaatgcct
     2341 ggttagtaat ttgattattt ttctgaagaa tatttagaat ttttctatca attttatcaa
     2401 gtatttcaac tggatatcct gtattgcttt catttggata attttttgtt ggcttgtttt
     2461 tcatatatta ccttttgttg caaaaataca ttataaaaag taatttttat aaataaaata
     2521 agaaatttat ttatatttgt tttatgttaa cttacctcca cacagatttg caggaggtta
     2581 ccaatgatta caatcagatt agctgaaatg aacgagattc aatttttaaa tcaattaatt
     2641 tcttgttcag caagagagct tagccaggaa gattatacaa aggaagaaat tgaaggagcc
     2701 attcaatatg tatttggtgt agacttagag ttaattttag acaaaactta ttttgttata
     2761 gaaaaggatg ggcaaattgc tggttgcgga ggatggagtc gaagaaaaac attattcggt
     2821 ggcagtcaat ataaaggcag ggagcaagga gtatatctgg atcctcaaca agattttgca
     2881 aaaataagag ctttttttat tcatcccaaa ttcgcaagac aaggattggg aagttctctg
     2941 ttaaaacatt gtgaacatga ggctttttta caaaaattta ctcggtttga gatgatggcc
     3001 actttaccgg gagttaagct ctatagcgct tttggttatc accctacctc aaatgaagtg
     3061 attactctcc ccaataatgt tccgttaaga tttgttcgga tgacgaaagg ttagatggta
     3121 attcatttta tactgtctgg agtcattatt gactcatggt tattgaaact taatttatcg
     3181 tcataatgtt aggaacggtt atcttaatga atacaatccg attagattaa taacgtaatt
     3241 taagttttac ttaagttatt tatgatatat tcatgaccac tttgtaaaca tttagtttat
     3301 ttttggtgtt ttctccagtt gagacaacag gaaaaattta gattataacc aggtgggata
     3361 aatatatgaa agagcaacaa aaggcaattt ttattgagaa tatcaaatcg acatgcgctg
     3421 ttgacgagca aatggctaaa gaattggctg attcattgct tgaaattcac cagttaaaca
     3481 ctggagttaa tgaacaaaca ctggttaatg aggtgttttt aactaaattt gctctctatc
     3541 ttaaaattgg gaaagaggtt gccttagatt gggttcgaaa aaataaggac ttggatactc
     3601 cgtttccttc tgatactgtt aaaagtcagc cagttcaaga tgcgtacaag cctgctgaga
     3661 acaaggttca ggtatatggg cctatgacaa ttggtaaatc tcctgttaaa acgactttaa
     3721 atcaattgac aggcaacttg ttggtggacg tggttgaggc cggaaaaaaa gagaaagctc
     3781 aaaaattacg agcttacata tttgaccaat tggcaaggcg attagaagct tcgcttagcc
     3841 aagagcagat taacgattta tataacagaa taagaggcac tggtaactat actaaaagcg
     3901 aatcattttc cgaagaacaa cttaaaattt taaaagaaaa aattgttcca gaattaaaga
     3961 gagagctaag tgacctcggc aacgggaatg ttaatatcct ggggttggac gttagtcgtg
     4021 aagataaata cgcatttgat acaaccaata tattttctgt ctggtttagc aataatccag
     4081 cggtttacat gccacaacat gttaaaactc aagtagaaaa aactgcaaaa cttaaccagc
     4141 caggcaaaac tcgaattgtt tttagctcat tatgcttgaa tgagacagcg cagattgatt
     4201 ttcagcaatg ggcaaaggaa aataacattg aattggttga tatagattca atagatttaa
     4261 aatcagtcag tgaaactgat gctcaattgc taaatctagc caaagatgag cttgaagcaa
     4321 tgagaaaggg caagggagga aatcctgctg cagcatccga tctggtgcgt tgggttgatg
     4381 taatcattgg agaaagcagt acttatattg atattgattt accaatgaat gataaaaaag
     4441 ttacagttga ggttcattct ggtttccctg ttctactcaa tatggggtca gcgctcacaa
     4501 aggatggtca acaacctgct atggaaaacc cggctttcaa tactgatatg attgcttata
     4561 gcaaagataa agaggcacgt cgtcaaataa tcgagggggt tgccaaaaag atcattgcgc
     4621 gttatgaaaa ctgcgcgaaa tatattgagg aaagtaaaaa cgaagagtta gtacgtttaa
     4681 aaaatacgcc aggttatcaa ttccttgtta aaaaaacaga cggcaaattt gatttatgta
     4741 ctttaagggc ggcagtttca gaagctcacc aggatgcttt gtcgtttgca actttttttg
     4801 gcgctgagta ttttgctaaa acttttgcta cacaagaact tgtacctgta attaaagagg
     4861 caattcagca tcaaaatcaa gatttgctta catctgttat tgaaaatcat atagaaaaac
     4921 aacatctcaa tgactatcct aagactcccg atggcattaa aaaactattg aagtcgtttc
     4981 aagggattgt ctataaacca ttggttatgg agttctcagg cccaagtgct gtctcatcat
     5041 cttgggtgga ggctatatct ggaagatcca tcccgaggaa ttttgaatat ttagctgaac
     5101 ctatgtctca accattacga gttttgcagc atcatgcctg tgtatctgga aaagctaatt
     5161 tttcttctga taacattcct aaatggtgtg aacttcagtc tgatgtagaa aaacgacaac
     5221 tacaaaggga agatggactt tcctggcttc ctgatgctca agagaagctt aagagggaag
     5281 ggcagcaagc caaacttgaa gaagagcaac gaatcaagct tgaagaagag caacgaatca
     5341 agcttgagga agagcaacga atcaagcttg aggaagagca acgaatcaag cttgaggaag
     5401 agcaacgaat caaacttgag gaagagcaac gaatcaagct tgaggaagag caacgaatca
     5461 agcttgagga agagcaacga atcaaacttg aggaagagca caaaagcaaa aaatactttg
     5521 ctcagtccga tgcaattgat ctcattttga ataattttga aaatgaaatt aactctattg
     5581 gtgcttatta tgctcctgca aaacaaagag ctatagagtt gctggatact ttgagaaaat
     5641 ataaagagga agcatttaat gatccaagca gagaaaaact aatctctttt gcgcaaaata
     5701 caaaaagagc aattcaggaa gcaacaccaa tactgcaaaa agatttaggt tggggagatt
     5761 atttagccaa tttagcgaaa caattggtta atgcagtgac ttttgctgtg gcttatgctg
     5821 taacttttgg aaccaccggg catcaaggct tttttgcttt gaaatcttct ctggcggtta
     5881 gtcaatctca aaatcttgat gaggaactca atagcaaatt aagtcaaaat aactgttagg
     5941 gaataggcca gatgtagaat gctaatgggc aagactttat ttttgctgcc caagcattct
     6001 tcttagatct aatgtatttc gattcggtaa ttttgattta gaaatgaaag attgcaatcc
     6061 atttcatttt gctgataatg tgcaaaatag attgtgtgag aacaatataa gcacatattg
     6121 tatatcgtaa cgatgttagg agagttagat gcgtagaagt aaagcaatgc aaatacctaa
     6181 aaaaagaaca caaattttaa ctatttcaga atcaagtaaa gaagatttgg atgttgtatc
     6241 cgagtattct gacgaactgg atgtttctcc caacaaagaa acaagcaaaa ctaacagtaa
     6301 aaaactggag agtactgccc gtaaagaaca gatcaagaaa ttggaaggag agctttacag
     6361 tcatattcaa gatgctatcg aattagaaga agaatctttt gcagaattca gcattaacac
     6421 tggaagacct agtaattatt tatttcgctt tgtcagtgaa aaagaatatt tggcaattct
     6481 tgaaaattta aatcaaggaa agagaatatt tggtaacaag aaaaaagtat ttgaagaaga
     6541 ggacgaaaag atatcaacta aagcaaaagg ggaaactttt tttgcttttg atcttaaata
     6601 tgctcaaaaa tatttcaata aaggtacaag gcgtgatgga gtgatgcttg cgatagaatt
     6661 gctcgaggaa gctgatacca aactattatc taatagtgaa attgttagaa ggatcggaga
     6721 tgatcctgga atggctggag gccgatgggc gaagtgtaag aaagtaaaac ccggcgaaag
     6781 ggggatagta gtagtcaagt gcgaaggtgt tggctcatgt aaatatccat ataccttagg
     6841 ttttagagaa ggggaagagg gtgtaagtcc ttttgatgta ctatttgagt taacgcgctc
     6901 tgtaaaagta ctgaacaaga atggcagttg taaaactatt tatcattatt ctgttcctac
     6961 accaaaagaa aaagagctta taaatattgt ggaagatgct gaaaatggta tagacgagat
     7021 tcatagtgat gaatttttgt cagttgtttc aagttctcct tcatcacgct ttggtttctt
     7081 tacaaacaag gagctctcgg gtatttcagg attggtaaat aaggcaccgg aacatataat
     7141 accaccctct gataatacct tctcgcatca ttaaatcagg ttaaattttt aaggctttag
     7201 acgggcaatt aaatactatt tatttgcccg tttctgcgta tcccctccat aacaagggtt
     7261 agccacatac aagtaaccca tcaactttat ttccattgct caatacaatt tacttattcg
     7321 ttggtttaaa ccaattaata tgagcgcatt gtactataat cttaataacc actatgtctc
     7381 tcatgttatt cattcacctg agaaaagagc caatcgtgat aacaatcctg agaaaattat
     7441 gaagaaacct ttggatacta ctgaacagaa tcgcattgct gcattatacg aattaaaaat
     7501 tctggataca ccagcagatg aaagatttga tcgaattatc cgactcactt cgggttactt
     7561 atcagttcct attgccttta tttcttttat tgattctaaa caccaatggt ttaaatccag
     7621 ctgtggcata ggccttaaca aaatatcccg gaatgattca ttttgccact acaccattca
     7681 gcaaagtaaa ccgatgatta ttccggacgc cttgttggat gagcgatttt gtaataatcc
     7741 ctatgtgact aattcaccaa atattcgttt ttatgcagga tttccattat ctacagttga
     7801 tggttttaat gtgggaacac tatgcgcaat agataaaaaa ccacgtaatt tagacgagac
     7861 tgaaattcaa attttaaaag atttggcaac tttggcagaa gatcaagtca atctgcttga
     7921 tatttcgctt cttgagagag ctgtacgaga aaaaaacatt tcgctgacaa aagctaagaa
     7981 agaacttgaa atgcgtaaca attttattcg taaagtattt ggtagcttta tgtctgacga
     8041 aattgttgca gcacttcttg aatcaaaatc agagttaaaa ttaggggggg aggagcgcaa
     8101 aattacaatt ttgtttagcg acttaagaaa ttttactact ctgtcggagc attttcctgc
     8161 ggataaaatt gttgctgcgc ttaacaatca ttacggaaaa atggttgatg tcatagaaaa
     8221 atacaatggc actgtcgatt cctttattgg tgatgccatt atggtggtat ttggagcacc
     8281 acattctgga ggggatgacg cgtacagagc gatagcttgt gcattagaga tgcaggcaac
     8341 catgcaagag gtgaacgcaa tgaatcgcaa cagcagttta cccgaattgg caatgggcat
     8401 agggattaat acaggttatg ctattgtagg taacattggt tctcagaaac gtatgcaata
     8461 tagtgcaatt ggttctccgg ttaatctggc atcacgcatc caagatctga ctttgggggg
     8521 gcaaatttta atttctgagg caacttacca ggaagctaat cattctcttg agttaaatgg
     8581 gcatctcaga gtaaaagtta agggaattgc tgctcctatc actatttatg atgtagtcgg
     8641 tctggataaa aaatcagatt catagacgtg ttaatat
//
LOCUS       DASLOE010000125         8653 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.126, whole genome shotgun sequence.
ACCESSION   DASLOE010000125 DASLOE010000000
VERSION     DASLOE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.126"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..531)
                     /locus_tag="U6309_002216"
     CDS             complement(<1..531)
                     /locus_tag="U6309_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505653.1"
                     /translation="MNTITEDSLTEEQKYMVQVWENHTHYEFYTKNVEDTMSTMEDNP
                     YVNNIPTMEGGFGKQEVKQFYSQFFIPQKPDDTEVELISRTVGTHQIIDELIFKFTHN
                     IRMDWILNGIEPTGNRVEIPLIAIVRFHNRKIISEHIYWDQASVLVQLGLLNSDKLPV
                     YRVEATNKIRELISRSL"
     gene            686..3688
                     /locus_tag="U6309_002217"
     CDS             686..3688
                     /locus_tag="U6309_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN5505654.1"
                     /translation="MIPRLTSSTATPRKIRLFLQQLEKIPDFHGEIDEALSSRIVLST
                     DNSVYQIIPEAIIFPKSENDISHIFKLASSEQFRDLTFTPRGGGTGTNGQSLNAGIII
                     DCSRFMNHIVEINPDEDWVLVEPGVVLDQLNEYLKPYNKFFAPALSPSNRATIGGMVN
                     TDASGKGSRLYGKTSQYILALTAVYLGGEVHTSEEIDVSLLDNMKKLPGQVGQFYKQL
                     DKTVQEHREAIDRQLPKLHRFVSGYNLAKIYSNDGKRFNANYILAGSEGTLAYVTKIK
                     LKLTNIPKYKCLFVVLYPTFSDALEHAKHLVTCDPEAIETMDSTILKLAKTDIIYHSL
                     KSFLSSGSNSQVDAINLIEFVANDEKELLHHAQLLKKNLKGTDFHFVTNYEDIINLWE
                     LRKRSVGLLGKVKGVRKPISGVEDTVVPPEYLADYVKEFRELLDSHGVSYGMFGHVDV
                     GCLHVRPELNLMDAKDREIYYEIIEKVAQLVHKYGGVLWGEHGKGFRSEFVPAFFGEE
                     LYNELRKIKSSCDPFNQLNPGKIAIPLGSHDSLASIRSEKTRADVDSQINYQFRDSYQ
                     LPLNCNGNGACFDYKLQDVMCPSYKISRDRIHSPKGRASLVREWLRQLSALTGKRELT
                     WPLKKQLNTYKRNHGQYDFSHEVYTALEGCLGCQACRSQCPVNVNVPSFKAKFLQTYH
                     TRYSRSIKDYLIAYSERVGYFQSLFPELTNFCLSNKLVKYCLHKYAELVDLPLFSSPN
                     LKSLLKANNIPWLDLNHLPASEKTIIYIQDWVTSFYEAELVLKACQLIKKLGFQVYIL
                     PWFENGKTLHDNGMLQSFAKITRKNALMLKKLSDHGLTMIGLDPAMVLTYRYEYSEYL
                     HENYNSKILLIQEWLSQILGNEKINELPIKANEKLNQYLLLSHCTEQANCSEALIQWQ
                     KVFQFFGLTLVAEKTGCCGMAGAYGHESIHQLNSHGLFDLSWKNYFEDSTGLSNILVD
                     GYSCRAQVKRLTGLKASHPAEILLGLFE"
     gene            complement(3776..4432)
                     /locus_tag="U6309_002218"
     CDS             complement(3776..4432)
                     /locus_tag="U6309_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505655.1"
                     /translation="MKAVANRYHVLCQEKVFIRSLLSGFFLLFISFLIMNFAGKYASL
                     MAGNPVNDLILDNLPLRDVTVFHVNAAIIFWTIFTIYTLLNPAFIPFITKSAALFILI
                     RCAFISMTHLGAPPNQLAIPDNFSSLVLFDGDLFFSGHVGGPFLMMLIFWNKTILRYI
                     CLAAAVFFAYTVLIGHIHYSIDVFAAPFITYGIFHISQFLFASDYQRLKSELTSSTGN
                     "
     gene            complement(4429..5595)
                     /locus_tag="U6309_002219"
     CDS             complement(4429..5595)
                     /locus_tag="U6309_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN5505656.1"
                     /translation="MKILTVLDSYPPDLNGGAYFTHRLAKFLQAKGHEVLVICPSRSL
                     KQGYTSYEGVNLYGVRSWPALGYKNFRVCWPFFIKKGILKAITDFNPDVVHLQGKFFL
                     GGICYRACRKKGIPLIATNHFMPENFFHYTHLPKYFEKWFHRTTWNIVIDMLNHVKIV
                     TTPTHTAANLLKEVHIQKEIHVVSCGVDLQKFQPKQNANLIRQRYKIPDKPILLYAGR
                     LDKEKNLSIAIKAFYKARQSIDAHFVLTGRGAELQRLKKLVQTLNLTEHVTFTGYLSD
                     AEYPLVYSLANCFVNPGTAELQSIVALEAIASGLPLLAAKAMALPELVKEGVNGYLFD
                     PNDVETLSCYMVKILSDRTFSEQMGRESRKLSQEHDIKRTIEQYETLYQSMVSL"
     gene            5745..6728
                     /locus_tag="U6309_002220"
     CDS             5745..6728
                     /locus_tag="U6309_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEN5505657.1"
                     /translation="MSSAKIIGMGKYLPANIVFSSDLDKKLNLPEGSVQKKSGLVSRH
                     FARVSETTSYMASQAALHAVEQAGITLAEIDAIIGACGAGEQPIPCTAVLVQKQLGLD
                     NSGIACFDINSTCLSFLTALDAASYMLSGGRFNRILIVSSDIPSFGLDWSDLETCTIF
                     GDGAAACVVERSNDSSRILAAHMKTYSLGAAFCQVQAGGTFMPPASEYDKKYGLFKMD
                     GKRVFKLASQMIGPMQEELFAKASITLNDVDWVVPHQASLLAMHHIRKKLGIPGEKFV
                     DVYTTHGNQMAASLPSALCHLIHNHNLERGQLVYLLGTGAGLSAAGMILEY"
     gene            6730..7710
                     /locus_tag="U6309_002221"
     CDS             6730..7710
                     /locus_tag="U6309_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN5505658.1"
                     /translation="MISVVTGATGCLGLNLTRRLIKEGHAVIALGRNLQLGEIISKMG
                     ATFVPLDLKDLESLSLISQNADFIFHCAALSSPWGKYKDFYQANVIGTHNVVQATPSQ
                     ARLVYVSTPSIYFDFTEKHNIKENTLLPEKPVNYYVQTKLIAESIVDKAQLQHDLDVI
                     TIRPRGIFGPYDRAIFPRLLKAERQGVLPIIGSGNHLIDITFVENVVESLILAALADK
                     CYSGKKYNITNDEPRTFIDIISRMFSALNKPLKTRSIPYNQARFAAKFLEFLHRVLYL
                     KTEPKITEYGVGVLAFGQTLNIEEAKKDLKYKPIYSIDEGIMKFAEWYCS"
     gene            7695..8531
                     /locus_tag="U6309_002222"
     CDS             7695..8531
                     /locus_tag="U6309_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN5505659.1"
                     /translation="MVLFMNIEYQLFEAGYCKHCERMTLKNGKIRQREYPSFCALIKH
                     PSHGYILFDTGYSDRFTSLTQKFPASIYRHLTPMTLVKSLKEQLLEIHIKPEDINYIV
                     ISHFHADHIGGLCDFPNAHFICHSDAIDDIQHTKGLKALLKGFLPGLLPEDFFERLLP
                     LQKTIQLDEHLLPFSDGFDVLGDSSLIAIPLPGHAKGQIGLYFKAPGDIFLVADSCWH
                     QETFKELIYPSALTYLIHHDKRAYLKTIQKLHELYQRNKTIEIIPSHCQQLRSRIGNP
                     LC"
BASE COUNT         2753 a         1507 c         1678 g         2715 t
ORIGIN      
        1 cagacttcgt gatatcagtt cccttatctt attggtggct tctactcgat aaacgggtag
       61 tttatctgaa ttgagtaaac cgagttgtac aagaacagat gcctgatccc aataaatatg
      121 ttcgcttatg atttttctat tatgaaatct gacaatcgca attaggggaa tttctacacg
      181 atttccagta ggctcgatgc catttaatat ccaatccatc ctgatattat gagtaaattt
      241 aaaaatgagt tcatctataa tttgatgagt acctactgtt cttgaaatca gttcaacctc
      301 agtatcatcc ggtttttggg gtataaaaaa ttggctgtaa aattgtttga cttcttgttt
      361 tccaaatcct ccttccatgg taggtatgtt attgacatag ggattgtctt ccatggtact
      421 cattgtatct tcaacgtttt tagtataaaa ctcataatgg gtatggtttt cccatacttg
      481 aaccatatac ttttgttctt cagtcagaga atcttctgtt attgtgttca tatcgtttta
      541 atcctttaaa aagatgcctg tatccttaat ttagaataaa aaaactattt tttctgattg
      601 aatagtagtt gtctttatga tttagcccta attatctggt agtctataat gagaagatta
      661 atggaggaaa agctaggaaa aaactatgat cccgcgtttg acctcttcca ctgcaacacc
      721 cagaaaaatc aggttatttt tacagcaact tgagaaaata cccgattttc atggtgagat
      781 tgatgaagct ttaagtagcc gaattgtttt atcaacggat aatagtgttt atcaaattat
      841 tcctgaagcc atcattttcc ctaaaagtga aaatgatata tctcatattt ttaaattagc
      901 cagtagcgag caatttcgtg atttaacttt cacccccagg ggtggcggca ctggaactaa
      961 tggccaatca ttaaatgctg gaataatcat cgattgttct cgtttcatga atcatattgt
     1021 ggagataaac ccggatgaag actgggtttt ggttgaacct ggagtagttc ttgatcaatt
     1081 aaatgaatac cttaagcctt ataacaaatt ctttgcacct gctttatctc caagtaatcg
     1141 cgctaccatt ggaggtatgg ttaatacaga tgcaagcggc aaaggctcaa gattgtatgg
     1201 aaaaaccagc caatacattc ttgcattgac agctgtctat cttggaggag aggtccatac
     1261 cagtgaagag atagatgtca gtcttcttga taatatgaaa aaattgcctg ggcaggtagg
     1321 gcagttttat aagcaattgg ataaaactgt ccaggaacat cgagaggcaa ttgataggca
     1381 attacctaaa ttgcatcgat ttgtctccgg ttacaatttg gcaaaaattt attcgaatga
     1441 tggcaagaga ttcaatgcca attatattct cgcaggttct gaaggcactt tggcctatgt
     1501 cactaaaatc aaattaaaat taacgaatat acccaaatac aaatgcctgt ttgttgtttt
     1561 gtatcctaca tttagcgatg ctcttgagca tgcaaagcat ctagtgacat gtgatccaga
     1621 agctattgaa acaatggata gcactatttt gaaattggcc aagacagata ttatttatca
     1681 ttctctgaaa tcttttctaa gctctggaag caacagccaa gttgatgcaa ttaatttgat
     1741 tgaatttgtg gcaaatgatg aaaaagagtt actacaccat gcgcaactgt tgaagaaaaa
     1801 tttgaaaggt acggattttc attttgttac taattatgag gatattatca atctttggga
     1861 gctaagaaag cgcagtgttg gacttttagg taaagtaaag ggcgtaagaa aacccatatc
     1921 gggtgttgag gatactgttg ttcctccaga gtatttggct gactatgtca aggaatttcg
     1981 tgagttactt gattctcatg gggtaagtta tgggatgttt ggtcatgttg atgtagggtg
     2041 tttacatgtt cgccctgaat tgaacttgat ggatgcaaaa gatagggaaa tctattacga
     2101 aataatagaa aaggtggcgc aacttgtcca taaatacggt ggggtattgt ggggcgagca
     2161 cggtaaagga tttcgaagtg aattcgtacc tgcatttttt ggagaggaac tctacaatga
     2221 actcaggaaa attaaatcaa gctgtgatcc tttcaaccaa ttaaatccag gaaaaattgc
     2281 catcccctta ggtagccatg attcacttgc cagcatccgt tcagaaaaaa ccagagcaga
     2341 tgtggacagt caaattaact accagttcag agattcttat caattacctt taaactgcaa
     2401 tggaaatgga gcgtgttttg attataaatt acaagatgtg atgtgccctt cttataaaat
     2461 aagcagggat agaattcatt ctcctaaagg aagagccagc ttggttaggg aatggttaag
     2521 gcaattaagt gctttgacag gaaagcgtga attaacatgg ccgttgaaaa agcaattaaa
     2581 tacttacaaa aggaatcacg gccaatatga tttttcccat gaagtttata ctgcgctaga
     2641 aggatgcctg ggatgccagg catgtcgttc tcaatgtccg gttaatgtca atgttccttc
     2701 gtttaaagca aaatttttgc aaacctatca cacacgatat tctcgctcaa taaaagatta
     2761 tcttattgct tacagcgaac gtgttggata ttttcaaagc ctgtttccag aattaactaa
     2821 tttctgtctc tcaaataaat tagtgaaata ttgtttacat aaatacgcag agttggttga
     2881 tctccctttg tttagtagtc caaatctgaa gtcactctta aaggcaaata atattccatg
     2941 gcttgattta aaccatttac ctgccagtga gaaaactata atttatatac aggattgggt
     3001 gaccagtttt tatgaagctg aacttgtgct gaaagcttgt cagttaataa aaaaactggg
     3061 gttccaggtt tatatattac cttggtttga aaatggtaaa actttgcacg acaatggaat
     3121 gcttcagtca tttgcaaaaa ttaccagaaa aaatgcttta atgcttaaaa agctatcaga
     3181 tcatggattg accatgatag gacttgaccc agccatggta ttaacctatc gatatgaata
     3241 ctctgaatat ttacacgaaa attacaatag caaaatcctg ttgattcaag agtggctctc
     3301 gcaaatactg ggtaatgaaa aaatcaacga gttaccaata aaagctaatg aaaagcttaa
     3361 tcagtattta ttgctaagtc attgtacaga acaggcaaat tgcagtgaag ctttaataca
     3421 gtggcagaaa gtctttcaat tttttggatt gactctagtt gctgaaaaaa cagggtgttg
     3481 tggtatggct ggagcttatg gacatgagtc aatccatcaa cttaattccc atggcttgtt
     3541 tgatttatca tggaagaatt attttgaaga cagtactggt ttatctaata ttttagtcga
     3601 tggctattca tgtcgagcac aagtcaaacg attaactggt ttaaaagctt ctcatcctgc
     3661 tgaaatatta ctgggattat ttgaataggt ataagctgga attgattatc tcagccaaat
     3721 atttactgaa ttactttctc atgcgggcta atcctacagt atggcgcaac cgttatcaat
     3781 ttccggtaga tgatgtaagt tctgatttta gccgttgata atctgatgca aacaggaatt
     3841 ggcttatatg aaatattcca taagtaataa atggggctgc aaagacatca atactataat
     3901 gaatatgacc aatcagcacc gtataagcga aaaaaactgc agcagcaagg caaatataac
     3961 gtaatattgt cttattccaa aagataagca tcattaaaaa agggcctccg acatgtccag
     4021 aaaaaaataa atctccatca aataaaacaa gagatgaaaa attatctgga atagccagtt
     4081 gattaggagg cgcaccgaga tgggtcatac taataaaggc acaacgtatc aatataaaca
     4141 gcgcggcact tttagtgata aaaggaatga aggctggatt taacagggta taaatagtaa
     4201 aaattgtcca aaagataata gctgcattaa catgaaaaac agttacatca cgtagaggta
     4261 aattatcaag aattaaatca tttaccggat taccggccat aagtgaagca tattttcctg
     4321 caaaattcat aatgagaaaa cttataaaaa gcaaaaaaaa gccactaagc agacttctta
     4381 tgaatacttt ttcctgacaa agaacatggt agcgatttgc gactgctttc ataaactcac
     4441 catcgattga tagagtgttt catattgttc gatagttcgt ttgatatcat gctcttgaga
     4501 taattttctg ctctctcttc ccatttgttc actgaaagtc cgatcactaa gaatcttgac
     4561 catataacaa gacaatgtct caacgtcatt agggtcaaag agataaccat ttactccttc
     4621 tttgacaagt tcaggcaaag ccatcgcttt tgcagccaaa agaggcaatc cagaagcaat
     4681 tgcttccaag gcaacaatac tttgtaattc agcagttccc gggtttacaa aacaatttgc
     4741 taaactatat accagcggat attccgcgtc tgatagatat cctgtaaaag tgacatgctc
     4801 agtgagattc agtgtctgaa ccagtttttt taatctttgt aattctgcgc cacgaccggt
     4861 taaaacaaaa tgagcatcaa tggattgtct tgctttataa aatgctttta ttgctattga
     4921 taaatttttt tctttatcca aacgaccagc atacaataga attggcttat cagggatttt
     4981 ataacgttgt ctaatgagat tggcattttg ttttggttga aatttttgta gatctactcc
     5041 acaagaaaca acatgaattt ctttttgtat gtgtacttct ttaagtaagt tagctgcagt
     5101 gtgggtagga gtagtgacta tctttacatg atttagcatg tcaatcacaa tattccatgt
     5161 ggtgcgatga aaccattttt caaaatactt gggaagatga gtataatgga aaaagttttc
     5221 tggcatgaaa tgattagtgg ctattaacgg gatacccttt ttacgacagg ctcgatagca
     5281 aattccacct aagaaaaatt ttccctgcaa atgaacaaca tcgggattaa agtctgttat
     5341 tgcttttaaa atacccttct tgatgaagaa aggccagcaa acacgaaagt ttttgtaacc
     5401 aagggctggc catgatcgta ctccatagag attgactcct tcataacttg tataaccttg
     5461 cttcaggctt cgagaggggc atatcacaag aacttcatgc cctttggctt gtaagaattt
     5521 tgccaaacgg tgagtaaaat aagcaccacc atttaagtcg ggaggatagc tgtccagtac
     5581 agtcaggatt ttcatgcata ttttagataa catataagat tagatagatt atcatttata
     5641 caaatacacg gcaattattt gccttccaac ctttaaaaag ttctctttga ctataataag
     5701 ccttatagta caataattgc tccatccttt aacaagtcag ttctatgtcg tcagcaaaaa
     5761 tcattggaat gggaaaatat ttacccgcaa acatagtgtt ttcatctgat ctagataaaa
     5821 aacttaatct tcctgaaggc agtgttcaga agaagtccgg attagtttcc aggcattttg
     5881 ccagagtcag tgaaacaact tcctatatgg caagtcaagc tgcattacac gctgtggaac
     5941 aagcaggaat tactttagct gaaattgatg ccattattgg ggcttgtgga gccggagagc
     6001 aacctatacc atgtacggcg gttctggttc aaaaacaatt gggattagat aattcaggaa
     6061 tcgcctgttt tgatattaac agtacctgtc ttagttttct cactgcactc gatgcggcat
     6121 catatatgct ctcagggggc agatttaatc gtatacttat tgtatcaagt gatatccctt
     6181 catttggtct ggattggagc gacttggaaa cctgtacgat ttttggtgac ggggcagctg
     6241 cttgtgttgt tgaaagaagc aatgactcca gtcgaattct tgctgctcat atgaaaacat
     6301 acagtttggg tgctgccttt tgccaggtcc aggctggagg aacattcatg ccgcctgcga
     6361 gtgagtacga taagaaatac ggtttattta aaatggatgg aaagcgtgta ttcaaactgg
     6421 ccagccaaat gattgggccc atgcaagagg agttatttgc taaagctagt ataactctaa
     6481 atgatgttga ttgggttgtc ccacatcaag ccagcttgct tgctatgcat catatacgta
     6541 aaaagcttgg catccctggt gaaaaatttg ttgatgttta cacaacgcat ggcaatcaaa
     6601 tggctgcttc tctgccttct gcattgtgtc atttgatcca taatcataat ttagaacgtg
     6661 ggcagttggt ttatttatta ggtactggcg ctggattgtc agctgctgga atgatactgg
     6721 agtattagta tgatttcagt tgtaacaggc gctacagggt gtttaggttt aaatcttaca
     6781 agacgcttga ttaaggaagg ccatgcagtc attgcattag gacgcaattt gcagttggga
     6841 gaaataataa gcaaaatggg agcaacgttt gttccactcg atttaaagga tctggagtct
     6901 ttgtctttga taagtcaaaa tgcggatttc atatttcatt gcgcggcttt atcaagcccc
     6961 tggggaaaat ataaagattt ttatcaggct aatgtcatag ggactcacaa tgtcgttcag
     7021 gcaactccca gtcaggctcg acttgtttat gtctccactc ctagtattta ttttgatttt
     7081 accgagaagc acaatattaa agaaaataca cttttaccag aaaaaccggt caattattat
     7141 gtgcaaacca aattaattgc tgaatccatt gtagataaag ctcaactaca gcatgatcta
     7201 gatgtgatta ccataaggcc cagaggaatt tttggaccat atgatcgcgc tattttcccc
     7261 cgtttattga aagcagagcg ccaaggggtg cttccaatca ttggttcagg taatcatttg
     7321 attgatatta cttttgtaga aaatgtagtg gaaagcctta tcctggctgc tttggcagat
     7381 aaatgttatt cagggaaaaa atataatatc accaatgatg aacctcggac ttttattgat
     7441 atcatttcaa ggatgttttc tgctcttaat aaaccattaa aaacaagaag tattccttat
     7501 aatcaagcaa gatttgccgc aaagtttctt gagtttttgc atcgggtgct ttatctgaaa
     7561 acagaaccaa agattacaga gtatggtgtc ggagtgttgg ctttcggaca aacattaaat
     7621 attgaagagg caaaaaaaga tttaaagtat aagccaattt atagcattga tgagggtata
     7681 atgaaatttg ctgaatggta ttgttcatga atatagagta tcagttattt gaagctggct
     7741 attgcaaaca ttgcgaacgc atgactttaa aaaatgggaa aatcagacag cgtgaatatc
     7801 catctttctg tgctcttata aaacacccaa gtcatggata tattttattt gacacaggat
     7861 attctgaccg ttttaccagc ttaacccaaa aatttccagc ctctatatat cgtcatttaa
     7921 ctcccatgac acttgtaaaa tccctaaaag aacagttgct agaaattcat ataaaacccg
     7981 aagatatcaa ttatattgtg atttcgcatt ttcatgctga tcacattgga ggattatgtg
     8041 attttcctaa tgctcatttt atatgtcatt ctgatgcgat agacgatatt cagcatacaa
     8101 aggggttgaa agcgttattg aaaggttttt tacctggact gctgcctgaa gatttctttg
     8161 agcgtttatt accacttcaa aaaacaatcc aacttgacga acatctttta cctttttctg
     8221 atggttttga tgtattgggt gacagctcat tgattgccat tcctttaccg ggacacgcta
     8281 aaggacaaat cggcttatat tttaaagcgc ctggcgacat tttcctggtt gcggacagtt
     8341 gctggcacca ggaaactttt aaagaactca tttatccgag cgctttgacc tatttgattc
     8401 atcatgacaa gcgtgcctat ttaaagacaa ttcaaaaatt gcacgaactg tatcagagaa
     8461 acaagactat tgaaattatt ccttctcact gccaacaatt gcgctcgaga attgggaacc
     8521 cattatgtta gcacgtattc tatttcatta ttttaataca ctttatctta ataaaactat
     8581 tactaccccg gaaaaattaa agactcatca agcaaaacaa tttaaaaaac ttataaaaaa
     8641 ttgtttgcat caa
//
LOCUS       DASLOE010000126         8544 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.127, whole genome shotgun sequence.
ACCESSION   DASLOE010000126 DASLOE010000000
VERSION     DASLOE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.127"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1566
                     /gene="sdeC"
                     /locus_tag="U6309_002223"
     CDS             <1..1566
                     /gene="sdeC"
                     /locus_tag="U6309_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdeC/LaiC"
                     /protein_id="HEN5505660.1"
                     /translation="AKFPTDKQWSAFKSGEAVRAKAQMDVLKQQIEKKIILQSQIIPA
                     LTECSEALDKQNVTEALQALNKLPAEKEIGKVKTIGQELRGQIVGVKQELTGNLESMQ
                     RATTAPIVQDAEKMKVRYETLVTDVTKRVTDFEKIKPANLESYNKAIADLNNMQQELT
                     LLRNEKIRMHTDKDKAVDFSDIEALEKRLQDVQSKLPTQLLEQTSKDVAKLTKMPEKI
                     TFNDIKSMTSKMNNYLETLELIRNDRIKKHAGSTDPLDMSDLDELKGQLQTYNQSMAD
                     ILLRAAKSSLDKIKDPATFEKESPYIKQCFDHLAELEKTLDDSDKGRKQKEDFTAYKS
                     ALMDKQEKAYPEMLQLQYKSEALIMQLRDICKIHHDNLAEARRVRLQQLDSQGGGLLG
                     GLWTVTNTIGLTTDTVNIERMQIRMKEQTLRAFKTELTNDKLNTDQVIAFLAKGSPSE
                     LQEALGISKENAEQLHGLLKQLEIKTASTDKLQEVEKLIDEVSTKIGKEPVKQDHTIT
                     IDEEESDDIRYGF"
     gene            1780..2901
                     /gene="dupA"
                     /locus_tag="U6309_002224"
                     /gene_synonym="laiE"
     CDS             1780..2901
                     /gene="dupA"
                     /locus_tag="U6309_002224"
                     /gene_synonym="laiE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector deubiquitinase DupA/LaiE"
                     /protein_id="HEN5505661.1"
                     /translation="MPIILDPEVLKVADYVYQERLSKPYTEVGPEWEYNHKTPYATRA
                     TGTGHNLQRFITIDDQKLHRPIHGLAHTMRTLFYSQLMYEAAKRQPHPHRCADGRTIA
                     DLSVQDLKKLNIAQLFFVAGRESEASYGDAYHRYHLYGAKQFEEYARKHLTHLFSEKE
                     IVLYSRCIEDRIGDRFDETAEGYLIHLSHMIDLMRCKSPVEVFIGHSRGVSGIVPTLI
                     QLFGREDGLDIMHYARSLFAATGEAVPYISSSEWPHLGIESDRVERALKIVGPLEVEG
                     QEADAKKTAQAGFSVDGCYGALVKVDTPDWYHQVKEKEDYDVDEVIALPPQITIREEP
                     PKANESFLLSLLRHVFWCCPSNQKRDDENTEVLEKKAQL"
     gene            2954..5578
                     /gene="sidJ"
                     /locus_tag="U6309_002225"
     CDS             2954..5578
                     /gene="sidJ"
                     /locus_tag="U6309_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS meta-effector polyglutamylase SidJ"
                     /protein_id="HEN5505662.1"
                     /translation="MFGFIKKVLDFFGVDQSEDNPSETAVETTDVSTKVKTTDTTQEE
                     SSVKTKTVVPTQPGGSVKPETIPPDQQRKHPIKTETTTGTTKQKSPKVTLMDGHVKQY
                     YFARRGETSTHDTSLPPPVKVLSGRSIPLKEIPFETTRNELVQIYLTSIDQFIKSNKL
                     NSVPSQQIASHYLFLRSLANSETDGIKKNQILSLAKPLGNYLASKEPHVWKMINELIE
                     KSEYPIIHYLKNNRAHSNFMLALIHEYHKEPLTKNQSAFVQKFRDSSVFLFPNSIYTA
                     WLAHSYDDDSSFNPMFRERLSTNFYHSTLTDNLLLRTEPKEVTLSSEHHYKKEKGPID
                     SSFRYQMSSDRLLRIQGRTLLFSTPKKDVVAVKVQKKGEPKSTLEEEFQMADYLLKHQ
                     RRLDVHSKLPQPLGQYSVKKSEILEISRGSLDFERFKTLIDDSKDLEVYVYKAPLSYF
                     TYLHDKNQDLEDLTTSVKTNVHDLFVLLREGIVFPQLADIFHTHFGEDEREDKGRYQA
                     LVQLLNVLQFQLGRIDKWQKAVEYVNLRSSGLADLGDSLPITSLFTSSDFTKHYFSEL
                     LTGGYHPTFFDKSSGTANSLFTGKRRLFGNYLYLNTIAEYLLVIQLTLGSYGDKVTRD
                     MMDKPKKEAVWRELANVMFTSCAEAIHIMTGIPQSRALTLLKQRANIEKHFRQTQFWM
                     TPDYSKLDEDALQMEQYSIYSGEPEYEFTDKLVSGVGLSVDGTHQDLGGYNRESPLRE
                     LEKLLYATVTLIEGTMQLDKEFFKQLQQVEKILSGEIKTDANSCFEAVALLLDLARPG
                     CHFQKRLVLSYYEEAKLKYPSAPTDAYDSRFQVVAKTNAAITIQRFWRETRKNLSEKS
                     DIESEKPESERTTDKRLI"
     gene            5857..>8544
                     /locus_tag="U6309_002226"
     CDS             5857..>8544
                     /locus_tag="U6309_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HEN5505663.1"
                     /translation="MPKYVEGVELTQEGMHAIFARMGHPEIKSGTIYNGVPTIDTEAL
                     NRQGFMPVLTGVGPKRDSGHWIMLIKGSGNQYHLFDPMGKVSGEGYQDILATQLPKGS
                     TLSVIPNEPGLNMGLCGYWVASVGLKARAELNKDNPPNLEALGQITTDAMKDELTDNG
                     YPKITGWLRAVSDKFPAGDPQPDAKALREATEKDLQINIPSSVPPVKDTSFKEIPVKP
                     TAPQQSSVPVWNGFSLFTDDTVRTAAKYAYDNYLGKPYTGPVESTPANIGGQMVYRQV
                     HGLAHTLRTMAYAEIIVEEARKAKLRGETLGKFKDGRTIADVTPEELKKIMIAQAFFV
                     TGRDDEESAKNYAKYHEQSRDAFLKYVKENESTLIPDVFKDQEEVNFYARVIEDKNHD
                     WDSTPAHVLINQGHMVDLVRVKQPPESFLQRYFSSMQRWIGTQATEAVFSIQRQFFHA
                     TYEAVTGFDSDNKEPHLVVSGLGRYVIGEDGKPIREAPKQGEKEGELKVFSQTYKLKE
                     NERFMRVDEFLKLPEIQSTFPGSGKYLEGGMPGMSYMDYWDRLNSRERARCENDVDFC
                     LKQLEIAHHKVKIDPLKEAFQSSTEKGKKGEKIEQERRQPNMDEIAAARIIQQIMANP
                     DCIHDDHVLINGQKLEEKFFRDLLAKCDMAVVGSLLNDTDVKNIDTLMRHEKDTEFHS
                     TDPKAVPVKIGDTWENKIRTKGGDVTQMKHDLIFLMQNDAWYFSRVNAIAQNRDKGSS
                     FKEVLFTALMTPLTNKSLMDTSHVPAPKKLYRGLNLPEEFKNKLINQANVMIANTTER
                     LFTDHSSEAFKQIKLNDFSQMSGRTCASTTTTYELTKAWDSNVVFEMLDPDGLLHPKQ
                     VGTHMAGSEDEFSVYLPEDVALVPVKVTFDGKTTNGEDRC"
BASE COUNT         2908 a         1729 c         1686 g         2221 t
ORIGIN      
        1 gcaaaattcc ctaccgataa gcaatggtct gcttttaaat caggagaagc agtcagagcc
       61 aaagctcaaa tggatgtatt aaagcaacag attgaaaaga aaatcatact gcaaagccaa
      121 attatcccag ccctcaccga atgtagtgaa gcgctggata aacaaaatgt cacagaagca
      181 ttacaagcgc tcaataaact gcctgcagaa aaggaaatag gcaaggtcaa gacaatagga
      241 caagagttaa gagggcaaat cgttggtgtc aagcaagagc taactggaaa tcttgaatcc
      301 atgcaacgtg caaccactgc gccaatcgta caagatgctg aaaaaatgaa agtacggtat
      361 gaaacccttg tcacagacgt gacaaaacga gtcaccgact ttgaaaaaat caaaccggcc
      421 aatcttgaaa gctataacaa agccattgcc gatttaaata acatgcaaca agaattaacc
      481 cttctgcgca atgaaaaaat tcgcatgcat acagataaag acaaagcagt ggacttttct
      541 gacatagaag cattagaaaa acgattacaa gatgttcaat ccaaattgcc aactcaactt
      601 ttagagcaaa cgtccaaaga tgttgcaaaa ttaacaaaaa tgccggaaaa gattaccttt
      661 aatgacatca aatcaatgac atccaaaatg aataattatc tggaaacatt ggaattgatt
      721 cgtaatgaca ggatcaagaa gcatgctgga tctaccgacc ctcttgatat gtcggatttg
      781 gatgaattaa aaggtcaact gcaaacttat aatcaaagca tggctgatat cctattgcgt
      841 gctgccaaaa gttctctaga caaaattaaa gatccagcta cttttgaaaa ggaatcccca
      901 tacatcaaac aatgttttga tcatttagca gaacttgaaa aaacactcga tgattcagac
      961 aagggaagaa aacaaaaaga agactttact gcctataaaa gcgcgttaat ggataaacag
     1021 gaaaaggcct atccagaaat gctacagtta caatacaaga gtgaagcatt gattatgcaa
     1081 ttacgtgata tctgtaaaat tcaccatgac aatttagctg aagcaagaag agtgagactt
     1141 caacaactag atagtcaggg aggtggactt ttaggtggat tatggactgt aaccaacacc
     1201 ataggtctta ctacagacac tgtgaatatt gaaagaatgc agattagaat gaaggagcaa
     1261 actcttagag cgtttaagac agaacttact aatgacaagc ttaatacgga tcaagtcatt
     1321 gcctttttag caaaaggaag tccatctgaa ttgcaagaag ctttaggaat atcaaaagag
     1381 aatgcagaac aattgcatgg attgttaaag caactggaga ttaaaacagc atcaaccgat
     1441 aaactccagg aagttgaaaa gctaatcgat gaagtctcaa ctaaaatagg gaaagaacca
     1501 gtaaaacaag atcacaccat cacaatagat gaggaagaaa gcgatgacat aaggtatggt
     1561 ttctaaggag tattttttaa gtgattgttc aattgttaaa tgatgtaatg aatagtgcaa
     1621 agctgtggca gatgagaaag caaaacatca tattatttgt ttctcatcaa gctgctgctt
     1681 tttattttaa ggaatactaa atttccttgt tgaataataa ttattcgtgt tgtagcatta
     1741 aagaatgcta tgttttaatt taaacaaaag gtggttattg tgcccataat tttagatcca
     1801 gaagtattaa aagtagcaga ttatgtctat caagagagat tatccaaacc ttacactgaa
     1861 gtaggcccgg aatgggagta caatcataaa accccttatg ccactcgcgc tacaggcaca
     1921 gggcataatt tgcaacgatt catcacgata gatgatcaga aacttcatcg ccccattcat
     1981 ggtttggctc ataccatgcg cactcttttt tacagtcaat taatgtatga agcagccaaa
     2041 agacagccac accctcatcg atgtgctgac ggacgcacta tcgctgatct gagtgtgcaa
     2101 gaccttaaaa aacttaacat cgcacaatta ttttttgtgg ctggacgaga aagcgaagcc
     2161 tcttatggtg atgcttatca ccgatatcat ctgtatggcg ctaaacaatt cgaagaatac
     2221 gcccgtaaac atttgaccca tctcttttca gaaaaggaaa ttgtccttta ttctcgatgc
     2281 atcgaagacc gaataggcga tcggtttgat gaaactgctg aaggctatct cattcactta
     2341 tcgcacatga tcgatttaat gcgctgtaaa agcccagtag aagtgtttat aggccattca
     2401 cgcggtgttt caggtatcgt accaaccttg attcaactat ttggtcgtga agacggttta
     2461 gatattatgc attatgcacg cagccttttt gctgccactg gcgaggctgt tccttatatc
     2521 agttcatcgg aatggcccca tttgggcatt gagtctgata gagtggaaag agcacttaag
     2581 attgtaggac ctcttgaagt ggaaggtcag gaagccgatg ctaaaaaaac cgcacaagct
     2641 ggattttcgg tggatggctg ctatggcgct ttggtaaaag tagacacacc cgattggtat
     2701 catcaagtta aagaaaaaga agactatgat gtcgatgaag tgatcgcttt gccaccgcaa
     2761 atcactataa gagaagagcc tcccaaggca aatgagtctt tcctgttatc actccttaga
     2821 cacgtatttt ggtgttgtcc ttcaaatcaa aaaagagacg atgaaaatac agaggtcttg
     2881 gaaaaaaaag cacaactata aagaggttat gaggcagctt attttataat tttcgaggtc
     2941 atgaggacgc agtatgtttg gtttcataaa gaaagtactt gatttttttg gtgttgatca
     3001 atctgaagat aatccatctg agactgcggt ggaaacaacc gatgtttcaa caaaagtcaa
     3061 aaccactgac accacccagg aagagagttc agtcaaaacc aaaacggttg ttcctaccca
     3121 acccgggggt tctgtcaaac cagagacaat cccacctgac caacaaagaa aacacccaat
     3181 aaaaaccgaa acgactacag gcacgactaa acaaaaaagc cctaaagtaa cactaatgga
     3241 tggtcatgtg aaacaatatt attttgcccg gcggggtgaa acaagcactc atgacacctc
     3301 gctgccacca cctgtgaagg tgttaagtgg gcgttctatc ccattaaaag aaattccttt
     3361 tgaaaccacc agaaatgaat tagtccagat ttatctcact tctattgatc aattcatcaa
     3421 aagtaacaaa ttaaactctg ttccatcaca acaaattgcc tctcactatc tctttcttag
     3481 atcacttgcc aactcagaga ccgatggaat taaaaaaaat caaatactgt cattagccaa
     3541 gccattgggc aattaccttg cctctaaaga gccccatgta tggaaaatga tcaatgaact
     3601 cattgaaaaa agtgaatacc ccattattca ttatttaaaa aataaccgtg ctcattccaa
     3661 tttcatgttg gcattaattc atgagtatca taaggaacca ttaactaaaa accaaagcgc
     3721 ctttgtacaa aaattcaggg actcctctgt ctttctcttc cctaactcaa tttatacagc
     3781 atggctcgct cattcctatg atgatgactc cagctttaat cctatgtttc gtgaaagatt
     3841 atccaccaac ttttatcatt ccactctgac cgataatctc ttgttaagaa cagaacctaa
     3901 ggaagtaact ctttcatcag agcatcatta taaaaaggaa aaaggaccaa ttgattcgtc
     3961 ttttcgttat caaatgtcaa gtgatcgttt attgcgtatc cagggcagaa ctttactgtt
     4021 tagtaccccc aaaaaggatg tagttgcagt caaagtacaa aaaaaaggag agcctaaatc
     4081 caccttggaa gaagaattcc aaatggcaga ttatttgctg aaacatcaac gtcgtttgga
     4141 tgtacacagc aaattaccac aaccgcttgg acaatactct gttaaaaaat cagagatttt
     4201 ggaaataagc aggggatcat tagattttga gcgttttaaa accttaatag acgactctaa
     4261 agaccttgaa gtgtatgttt ataaagcccc cctgtcttat ttcacttacc tacacgataa
     4321 aaatcaggat ttggaagatc tgacaacttc tgttaaaaca aatgtgcacg atctctttgt
     4381 tctattacgc gaaggaattg tgtttccaca attggccgat atcttccata ctcattttgg
     4441 tgaagacgag cgcgaagaca aaggaagata ccaggcatta gttcaacttt tgaatgtttt
     4501 acagttccaa ttagggcgca ttgataaatg gcaaaaagcc gtggaatacg tcaatttgcg
     4561 cagcagtggt ctggctgatt tgggggacag tctgcccata actagcctgt tcacttcatc
     4621 tgatttcaca aaacattatt tctccgaact gcttacagga ggatatcacc caaccttctt
     4681 tgacaagtca tctggtacag ctaactcttt attcaccggc aagcgccgat tatttggtaa
     4741 ttacttgtat ctcaatacca tagcagaata tttgctggtt attcagttaa cacttggtag
     4801 ctatggggat aaagtgactc gagacatgat ggataaacct aaaaaagaag cggtatggcg
     4861 agagttggca aacgtcatgt ttacaagttg tgcagaagcc attcatatca tgactggaat
     4921 acctcaatcg cgagcactga cactactaaa acaacgcgct aatattgaaa aacatttcag
     4981 acagacacaa ttctggatga cgcctgatta ttccaaactg gatgaagatg cactgcaaat
     5041 ggaacaatac agcatttatt ccggtgagcc agaatatgaa ttcactgata aattagtctc
     5101 tggtgttgga ttgtctgtcg atggcactca tcaggacttg ggtggatata acagggaaag
     5161 cccattaagg gagcttgaaa aactgctcta tgccacagtg acactgattg aaggaaccat
     5221 gcaacttgat aaagaatttt ttaaacaatt acagcaagtc gaaaaaatac tttctggtga
     5281 gataaaaact gatgccaatt cgtgctttga agcagttgcc ctacttctgg accttgcaag
     5341 gcctgggtgc cattttcaga aaagattagt tttatcctac tacgaagaag ccaagcttaa
     5401 atatccctcc gcaccaactg acgcctatga ttcacgtttt caagtagtcg ccaaaaccaa
     5461 tgccgcaata accattcaac gattctggcg agaaactcgc aaaaacttgt cagaaaaatc
     5521 ggatatcgaa tctgaaaaac cggaatccga acgtactact gataaacgtt tgatataaga
     5581 cagacaatct gagcctctaa cttcttgaaa tcacactatt cccatggaga agtcgataag
     5641 acaggataga cttcttcatt ttcagacttg tacttttaga aaaataagag aaagccaccc
     5701 acctctgttc cagctatcaa ctcgatatgt tggccatttt ttaagcgctt aatactttct
     5761 taatgctgta gtcctataat gaaaataaaa taagaatatt cacatctttt agagccagta
     5821 tttgtacaca caaatatccg tttgggagaa agtaaaatgc ctaaatatgt agaaggggta
     5881 gaattaactc aagaaggcat gcatgctatt tttgcacgga tgggacatcc cgagattaaa
     5941 agcggtacta tttataatgg agtaccaact attgacacag aagccctgaa cagacaaggc
     6001 ttcatgcctg ttttaacagg ggtaggacct aaaagagact caggtcactg gatcatgtta
     6061 atcaaaggat caggaaacca ataccatcta tttgatccaa tgggtaaagt ttcaggtgaa
     6121 ggctatcaag acattttagc cactcaatta cccaaaggct ccaccctttc tgttatcccc
     6181 aatgagcctg gattaaacat ggggctatgc gggtattggg tcgcctctgt tgggctaaaa
     6241 gcacgtgctg aacttaacaa agacaatcct cccaatttag aagcccttgg tcaaatcact
     6301 acggatgcaa tgaaggatga attaaccgac aatggttatc cgaaaattac cggctggtta
     6361 agggctgtct ctgataaatt tccagcaggt gatcctcaac ctgatgccaa agcattaaga
     6421 gaggctacag aaaaagattt acagattaat atcccctcct ctgttcctcc ggttaaagac
     6481 acctctttta aggagatccc tgttaaaccc actgcccctc aacagtcatc agtacccgtt
     6541 tggaatgggt tttcattatt cactgacgac acagttcgca ctgctgcaaa atacgcctat
     6601 gacaattatc tggggaaacc ttatactggc ccagtggaat caacaccagc caatatcggc
     6661 gggcaaatgg tatacagaca agtccatgga cttgctcata ctttacgaac aatggcttat
     6721 gcagaaatca ttgttgaaga agcacgtaaa gccaaattac gaggggaaac attaggaaaa
     6781 ttcaaagatg gccgcactat tgctgacgta actcctgaag aactgaaaaa aattatgatt
     6841 gctcaggctt tcttcgttac aggaagagat gatgaggaat ctgcaaaaaa ttatgccaaa
     6901 taccatgagc aaagccggga cgcttttcta aagtatgtga aagaaaatga atccaccctg
     6961 attcctgatg tcttcaaaga tcaggaagaa gtcaattttt atgcccgagt gattgaagat
     7021 aaaaatcatg attgggattc aaccccagcc catgtactga ttaaccaagg ccatatggtc
     7081 gatttggttc gggtaaaaca acctcctgaa tcattcttgc agcgttattt cagttccatg
     7141 caacgttgga taggtaccca ggcaactgaa gccgtttttt caatacaaag acagtttttt
     7201 catgccacct atgaagcagt gactggcttt gactccgaca acaaagaacc tcatctggtt
     7261 gtttctggct tgggacgtta tgtcatcggt gaggatggta agcccataag agaagcacct
     7321 aaacagggag aaaaggaagg tgagttaaaa gttttctctc aaacttataa attgaaagaa
     7381 aatgaacgat ttatgcgagt ggacgagttt ttaaaacttc ccgaaatcca aagtactttc
     7441 cctggttctg gtaaatattt ggaaggcgga atgcccggca tgagttacat ggattactgg
     7501 gatagattga actccagaga aagagcacga tgtgaaaacg acgtcgattt ttgtttaaaa
     7561 caattagaaa tagcccatca caaagtcaaa attgatcctc tgaaagaggc atttcaatcc
     7621 agcacagaga agggaaagaa aggagaaaaa atagagcaag aaagaaggca acccaacatg
     7681 gatgaaattg cggccgcccg catcattcag caaatcatgg ccaatcctga ttgcatccat
     7741 gacgatcatg tgctcatcaa tggccagaaa ctggaagaaa aattcttccg tgacttgtta
     7801 gccaaatgcg atatggctgt tgtaggctca ttgctcaatg acaccgacgt caagaatatt
     7861 gatactttaa tgcgacacga gaaagacacc gagtttcact ctaccgaccc caaagcagtt
     7921 ccagtgaaaa ttggagacac ctgggaaaat aaaataagaa ccaaaggtgg cgatgtcact
     7981 caaatgaagc atgatttaat tttcctcatg caaaatgacg cctggtactt tagccgagtc
     8041 aatgccatcg cacaaaaccg ggataaaggc tcctctttca aagaagtatt gtttaccgcc
     8101 ttaatgacgc cattgactaa taaatcatta atggatacat cccacgtccc agctcctaaa
     8161 aaactgtatc gtggattaaa tcttccagaa gagtttaaaa ataaattaat caatcaagcc
     8221 aatgtgatga ttgcaaatac aacagagcgc ctcttcacag accactcctc ggaagccttc
     8281 aaacaaatca agttaaatga cttcagtcaa atgtctggta gaacctgtgc cagtacaacc
     8341 acaacttatg aacttacaaa agcctgggat tccaatgttg tttttgaaat gcttgacccg
     8401 gatggtttac tgcatccaaa acaggtagga acccatatgg ctgggtcaga agatgaattt
     8461 tccgtctatt taccggaaga tgttgctctg gtccctgtca aagtcacttt tgatgggaaa
     8521 acaacaaacg gagaagatcg gtgt
//
LOCUS       DASLOE010000127         8534 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.128, whole genome shotgun sequence.
ACCESSION   DASLOE010000127 DASLOE010000000
VERSION     DASLOE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.128"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1491)
                     /locus_tag="U6309_002227"
     CDS             complement(<1..1491)
                     /locus_tag="U6309_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEN5505664.1"
                     /translation="MIILYDFLQESSVMPRSNNDSKHKKKSAPQSKFKEQQLNHGSKE
                     HKSKFKFSQRKVNKKRVGMSHLPGNIYTLFTPVNGPKSKDEWIDTSKIMVDLHIDNMS
                     SSDYIPSAIDRTDLVIVQPVHLLRKTSGRGLFAREDIPKGTCIGIYTGEVYSEQEFEQ
                     YLIEHAGSDKSYAMYVGGRVVDAARKGNLTRYINFSDSQDNAEFVETTLNRKKVAKVI
                     TTKNIKAGQQLLINYNTYEEKASRYYYFLNPGDGWLSAQEFYQTYQSQYQLEQMPYNL
                     EGFDLKAGDRVLMTQIGRIIFANYSLAKEQELNASDIDLPFLKVGSDEKILDFDEADT
                     FTPLMAACYLGQVENVKWLIEHGANIDQQQSHSGHCPLSLTLKGYSLAKDTQKYIDII
                     QLLIKNEVNLLVHDRSDKTFLHNAALVLNNLDFQSVVKFLIGQNPIDINEYFTYIDEN
                     DFDIVMHCYNNKLFNKALVLLAFYPDYFKRNYMSDNEGHNQFNINAF"
     gene            1635..2399
                     /gene="map"
                     /locus_tag="U6309_002228"
     CDS             1635..2399
                     /gene="map"
                     /locus_tag="U6309_002228"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN5505665.1"
                     /translation="MGVTIKTPDEIEKMRVAGRLAAEVLEMIGPYVQVGITTDELNTI
                     CHDYIVNVQKAIPAPLNYNGFPKSICTSINHVVCHGIPGKKTLKDGDIINIDVTVIKN
                     EYHGDTSKMFIVGTPSVKAKHVVQIAHECLYIGIDMVKPGVQLGDIGHAIQQHAEKNR
                     CSVVRDYCGHGIGRVFHEDPQVLHYGVPGTGMKLEPGMTFTIEPMVNIGKHHTRLLPD
                     HWTVVTKDHSLSAQWEHTLLVTDTGVEILTLRNEER"
     gene            2386..4971
                     /gene="glnD"
                     /locus_tag="U6309_002229"
     CDS             2386..4971
                     /gene="glnD"
                     /locus_tag="U6309_002229"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEN5505666.1"
                     /translation="MKNDNRIIKNTIKQFKEKLCKDFSQKANITSITRKLAVFIDTIL
                     IQLFIKNKLHFGGNFCLLALGSYGRRELQLHSDIDLLILHTEKVSNIQLQRAQKFIQD
                     CWDVGLEVSHQITTVSSCANLASQDLSVISTIMDMFLLCGHGALMEELIYQTHTLHMW
                     PSHQYFFAKLQEQQNRYAKYGETAYNLEPNIKNGPGGLRDLQILLSISKRHFKIKKLA
                     EGIGYGFITDKEYEELKYCQNFLWRVRFALHMLAGKPEERLSFDYQVKLAQFFGYQDQ
                     SHILAIEQFMKDYFKVIKRNRELNEMLLQWFNETIVYHQKQKIIRLDDEFQLSNRFIE
                     VRNNRVFKQNPQSILKLFYWLVKRPDIEGVRASTIRLIRESLFLMGKRFRESKETANI
                     FVNIFRTGNDPYDALQRMNRYGVLGHYLDCFATVTGQMQYDLFHAYTVDQHTLFVIRN
                     ISRFKKNEYAKQFPLCAKIITALEKPEILYLGALFHDIAKGRGGDHSELGAIEAQQFT
                     QRHYMEAEDSKLIVWLVRYHLLMSQTAQRKDIYDPKTIEQFCQLLPHARYLDYLYLLT
                     VADICGTNPTLWNAWKDSLLKELYHAAKTRLHKQQELLDEAALISIRKQYAMDILISD
                     GISSRVIQDLWSQFKGKYFLHESPEVIARHTKAILNSKQFPVVIIMPHHSQGGTEVFI
                     YMPHKDERFTITTSVLSNHHVTIQEAAIITCDNQFDLDTYIILDENNQAFLNEQRARD
                     IQKSLCDHLASTGRLPAVSRRRLSRALTHFNVKTQINFIDDNTNHQTQLFLVTNDRPG
                     LLATISRVFLTLNIHLHNAKIATAGERVEDMFYISNQTGYSLNHEEKTILKEKLILEI
                     SKSKY"
     gene            complement(4993..5436)
                     /gene="tadA"
                     /locus_tag="U6309_002230"
     CDS             complement(4993..5436)
                     /gene="tadA"
                     /locus_tag="U6309_002230"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEN5505667.1"
                     /translation="MIDKFWMQLAYEQAVIARNEGEVPVGAVLVSKDNQLLGVGRNVI
                     EKSHDPSDHAEIRAIRQASRKLNNHRLLDTTLYVTLEPCVMCAGLMVHARIKRLVFAT
                     RDFKAGAAGSMFNVLHALNHSVLIDEGIMQAECSQLLSDFFRNLR"
     gene            complement(5433..7010)
                     /gene="guaA"
                     /locus_tag="U6309_002231"
     CDS             complement(5433..7010)
                     /gene="guaA"
                     /locus_tag="U6309_002231"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN5505668.1"
                     /translation="MNDLKKSPLLILDFGSQYTQLIARRVREMGVYCEIYPYHINHEQ
                     FKKLNPCGVILSGGPSTVTHDANPRAPQWLFESDLPLLGICYGMQTMAVQLGGQVHSS
                     ALREFGYAELRLHGHSQLLSNIEDRTAVDGSALLDVWMSHGDKVTELPPGFKVICETR
                     NAPIAGMADESRQMYGLQFHPEVTHTLQGLRILQRFVVDICKAPTDWTPEHIIDEAIN
                     KIREQVGTEKVLLGLSGGVDSSVVAALLHRAIGEQLVCVFVDTGLLRLNEAEQVLSMF
                     GRHMGIRIIAVNAEDKFLTALKGVTCPEEKRKIIGRTFIEVFDEEAQKLTDIKWLAQG
                     TIYPDVIESAATSTNDAAVVIKSHHNVGGLPDTLNLKLLEPIRELFKDEVRQVGLELG
                     LPHDMVYRHPFPGPGLGVRILAEVKKEYADILRKADAIFIEELHNAQLYHKISQAFAV
                     FLPVKSVGVMGDGRRYDYVICLRAVETVDFMTAHWSQLPWDFLGKVSNRIINEVEGVS
                     RVTYDISGKPPATIEWE"
     gene            complement(7013..8485)
                     /gene="guaB"
                     /locus_tag="U6309_002232"
     CDS             complement(7013..8485)
                     /gene="guaB"
                     /locus_tag="U6309_002232"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEN5505669.1"
                     /translation="MPLSIVQQSLTFDDVLLVPAHSTILPKDVSLKTKLTRAIHLNMP
                     LISAAMDTVTEARLAIALAQEGGLGIIHKNMSIADQAEEVRRVKKFESGMVKDPISVT
                     PDLTVKELLAVMTKYNFSGVPVVDGKHLVGIVTSRDIRFETNMNLTVAQVMTPKGRLV
                     TVKEGASREEVRSLLHKHRIEKLLVVNENFELRGLITVKDIQKAKDNPYACKDSFEQL
                     RVGAAVGVGEGTDERVAALVESGVDVIVVDTAHGHSQGVLNRVKWIKKNYPDVQVIGG
                     NIATAAAARDLYEAGADAVKVGIGPGSICTTRIVTGVGIPQISAIANVAQELKGIIPV
                     IADGGIRFSGDVCKALAAGADTVMLGSMFAGTEESPGEIELYQGRTYKNYRGMGSIGA
                     MSLAQGSSDRYFQDASLGTEKLVPEGIEGRVPYKGPVQTIIHQLLGGLRSCMGYTGCA
                     TIEELHSKTEFVQVTNAGMRESHVHDVSITKQAPNYQVDN"
BASE COUNT         2661 a         1778 c         1471 g         2624 t
ORIGIN      
        1 aaaagcatta atattaaatt gattatggcc ttcattatca ctcatataat ttcttttaaa
       61 ataatccgga taaaaagcta ataaaacaag agctttatta aataatttat tgttataaca
      121 atgcatgacg atatcgaaat cattctcatc gatgtatgta aagtattcat tgatatcaat
      181 aggattttgt cctattaaaa attttacaac agattgaaaa tcaaggttat tcagtactaa
      241 tgcggcatta tgtaaaaaag tcttgtctga tcggtcatgc actaaaagat taacctcatt
      301 tttaatcaat agctgaatga tatcaatgta tttttgggta tccttcgcta aagaatatcc
      361 tttcaatgtc aaactcaaag gacaatgacc agaatgactt tgctgttgat cgatattagc
      421 accatgttca ataagccatt ttacattttc aacttgtcca agataacagg cggccatcaa
      481 tggggtgaat gtatccgctt catcaaaatc taatattttt tcgtcagaac ccacttttaa
      541 aaatggcaaa tcgatatctg aggcatttaa ttcttgttct tttgcaagag agtagttagc
      601 aaaaattatc cgtcctattt gagtcattaa aactctatcc cctgctttaa gatcaaatcc
      661 ttccaaatta tagggcattt gttctaattg atactgagat tggtaagttt gataaaattc
      721 ttgtgctgat agccaaccat cgccaggatt aagaaagtaa taatacctgg aagccttctc
      781 ttcgtaggta ttatagttga ttaatagttg ttgtcctgcc ttgatgtttt tagtggttat
      841 aacttttgct actttcttgc gattcaatgt agtttcaaca aactctgcat tatcctgact
      901 atccgaaaaa ttgatataac gagtcaaatt accttttctt gctgcatcga ctacccgtcc
      961 cccaacatac atcgcataac ttttatcaga accagcatgt tcgattagat attgttcaaa
     1021 ttcttgttca gaatacactt ctcctgtgta aataccaata caagtcccct ttgggatatc
     1081 ttccctggca aataaacctc gcccactggt ttttctgagt aaatggacag gctgaacaat
     1141 aacaagatca gttctgtcta tagcagatgg aatgtaatca ctgcttgaca tgttatcaat
     1201 atgcaaatca accataattt tgctcgtgtc aatccattca tccttacttt tcggtccatt
     1261 gacaggagta aacagcgtat aaatattacc tgggagatgt gacattccaa ctcttttttt
     1321 attaaccttt ctttgggaaa atttaaattt agatttgtgc tctttactac catgattcaa
     1381 ttgctgttct ttaaactttg actgaggggc acttttcttt ttatgtttgc tatcattatt
     1441 gcttcttggc ataacactgc tctcttgtaa aaaatcatat aaaataatca ttaaggagct
     1501 attatactct attagagtat tttttgctct attttgcgtg tgattttcat tgaaaatcta
     1561 ttattttgct gattgaagag tattgttgct cctcttaccc atgtgctaac atttatgatt
     1621 ttggaaaaac cataatgggc gttacaatta aaactcctga tgaaattgaa aaaatgcgtg
     1681 tcgctggaag actggcagcc gaagtacttg agatgattgg tccttatgtt caagttggca
     1741 ttacaactga tgaattaaac actatttgcc acgattatat tgttaatgta caaaaggcta
     1801 ttcccgcccc tttaaattat aacggtttcc ccaaatcaat ttgcacctca attaatcatg
     1861 tggtatgtca tggaatacca gggaaaaaaa ctttaaaaga tggagacatc atcaatattg
     1921 atgtcactgt tataaaaaat gaataccatg gcgataccag taaaatgttt attgtaggca
     1981 ccccttctgt caaggctaag catgtagtgc agatagcaca tgagtgcttg tatattggaa
     2041 ttgatatggt taaacccggt gtccagctag gtgatatagg gcatgccatt cagcaacatg
     2101 cagaaaaaaa tcgttgctct gtggtgcgtg attactgcgg acatggaatt ggccgtgtat
     2161 ttcatgagga tccacaagtg cttcactatg gagttcccgg aacagggatg aaattagaac
     2221 cagggatgac ttttactata gagccgatgg ttaatatagg gaaacatcac actcggctat
     2281 taccagatca ttggactgtc gttaccaaag atcatagtct ttcagctcaa tgggagcaca
     2341 cacttttggt aactgataca ggtgttgaaa ttctgacttt acgtaatgaa gaacgataat
     2401 cgcatcataa agaatacgat taaacagttc aaggagaagc tgtgcaagga ctttagccaa
     2461 aaagctaata ttacctccat cacacgcaag cttgctgtat ttattgatac tattttaatt
     2521 caattattta ttaaaaataa gttacacttt ggaggtaatt tttgtctcct ggctcttgga
     2581 agctacgggc gtagagagct tcaactccac tcagatattg acttgttaat attgcacact
     2641 gaaaaagtaa gcaatatcca attacaacgc gcacaaaaat ttatccagga ttgttgggat
     2701 gtggggctgg aggtaagcca tcaaattacc actgtttctt catgtgccaa tttggccagt
     2761 caggatttga gcgtaatttc taccattatg gatatgttct tactgtgtgg gcatggtgcc
     2821 ttaatggagg aattaattta ccaaacacac accttgcaca tgtggcctag ccatcaatac
     2881 ttctttgcca aacttcagga acagcaaaat cgttatgcaa agtatggaga aacagcctac
     2941 aaccttgaac ctaatattaa aaatggtcca ggtggattaa gagacctgca aattttgcta
     3001 agcatcagca aacgccattt taaaattaaa aaattagccg agggaatcgg ttacggcttt
     3061 attactgata aagagtatga agaattaaaa tactgccaga actttctttg gcgcgttcga
     3121 tttgctttac atatgttagc gggaaagcct gaggagcgct tgtcatttga ttatcaggtt
     3181 aaattagctc aattttttgg atatcaagat cagtctcata tcttggccat agagcaattt
     3241 atgaaagact actttaaagt catcaaacgc aatagagagc tgaatgaaat gctcttgcaa
     3301 tggttcaatg aaaccatagt ctatcatcaa aagcaaaaaa ttattcgtct ggatgatgaa
     3361 tttcaactgt caaaccgatt cattgaagta cgcaataacc gtgtatttaa acaaaatccg
     3421 caatctatat tgaaactatt ctattggctt gttaaacgtc ctgatataga aggagtaagg
     3481 gcaagcacaa tcagactaat aagagaatca ttatttttaa tgggcaaacg ctttagagaa
     3541 tcaaaagaaa ccgctaatat atttgtcaac atattcagaa ctggtaatga tccctacgat
     3601 gcattacaaa gaatgaatag atatggtgtt ctagggcact atcttgattg ttttgctaca
     3661 gtcactggac aaatgcagta tgatttattc catgcctata ctgttgatca gcatacattg
     3721 tttgtgatcc gtaacatatc tcgctttaaa aagaacgaat atgccaaaca atttccttta
     3781 tgcgccaaaa tcattactgc tttagagaaa cccgaaattt tgtatctcgg cgctttattc
     3841 catgacattg ccaagggtcg gggaggagat cattctgaat taggagcaat tgaggcacaa
     3901 caatttacac aacgccacta tatggaggca gaagatagca aattgattgt atggcttgtt
     3961 cgctaccatt tactcatgtc acaaactgct cagcgtaaag atatttatga tcctaaaacc
     4021 attgagcaat tctgccaact tctgcctcat gcaaggtatt tggattatct ttatttactc
     4081 acagtagcag atatttgcgg tacaaatcct actctttgga atgcctggaa agattcattg
     4141 cttaaagaat tatatcatgc agcaaaaaca agacttcata aacaacaaga gttgctcgat
     4201 gaagcagctc tgatatccat tcgtaaacaa tacgctatgg atatattaat ttcagatggg
     4261 atatcctcca gagtcataca ggatttatgg agtcaattta agggaaaata ttttcttcat
     4321 gaatctcctg aggtgatagc cagacacacc aaggccatac tgaacagcaa acaatttcca
     4381 gtggtaataa ttatgccaca tcacagtcag ggtggaaccg aagtatttat ctacatgcca
     4441 cataaagatg agagatttac tatcaccact tcagtattaa gcaatcatca cgttactata
     4501 caggaagcag caattattac ttgtgataat caatttgatc tggatactta tattatttta
     4561 gatgaaaaca atcaggcttt tttgaatgaa caacgtgcta gagacatcca aaaaagtcta
     4621 tgtgatcatc tagccagtac aggacgcctc cctgctgttt ctcgcagaag attatccaga
     4681 gcattgactc attttaatgt caaaacacaa attaacttta ttgatgacaa tacaaatcat
     4741 caaacccaac tatttctagt taccaatgac agacctggac ttttggcgac tataagccgt
     4801 gtgtttctta cactaaacat tcatttacat aatgcaaaaa ttgctacggc tggtgagcgg
     4861 gtagaagata tgttttacat ctccaaccag acaggttatt ccctgaatca tgaggaaaaa
     4921 acaattttaa aagaaaaatt aattttggaa atttctaaaa gcaagtatta agaaatatgc
     4981 caagataaat tactaacgaa ggtttctgaa aaaatctgac agcaattgag aacactcagc
     5041 ctgcataatg ccttcgtcta ttaataccga atggtttaga gcatgtaaaa cattaaacat
     5101 tgagccagct gcccctgctt taaaatctcg ggtagcaaat accagtcgtt tgatgcgtgc
     5161 gtgaaccata agtcccgcac acataacaca gggttcaaga gtaacataaa gcgttgtatc
     5221 caataatcta tgattattta actttctcga ggcttgtcgt attgccctga tttcagcatg
     5281 atcagaagga tcatgacttt tttcaatgac atttctgcca acgcctaata attgattgtc
     5341 tttgctaacc aacacagccc caacaggaac ctctccttca ttacgagcta taaccgcttg
     5401 ctcataagcc agttgcatcc aaaatttatc aatcattccc attcaatggt agcaggtggc
     5461 ttgccagaaa tatcgtaagt gacacgtgat actccctcta cttcattaat aattcgattg
     5521 gatacttttc ctaaaaaatc ccagggcaat tgagaccaat gtgctgtcat aaaatcaact
     5581 gtctcaacag ctcttaaaca aattacgtaa tcatatcggc gcccatcccc catcacaccc
     5641 acgctcttta ctggtaaaaa aacagcaaat gcctggctga ttttatgata aagctgcgcg
     5701 ttatgcagtt cttcaataaa aatagcatcg gctttacgta atatgtccgc atattctttt
     5761 tttacttccg ctaaaattct tacacccaat ccaggtccag gaaagggatg ccgatagacc
     5821 atatcatgcg ggagtcccaa ttccagacct acttgacgta cttcatcttt aaagagctca
     5881 cgaataggct ccagcagttt aagatttaat gtatctggta aaccaccgac attatgatga
     5941 gactttatga ccacagccgc atcattagta cttgttgctg cagactcgat gacatcggga
     6001 tagattgtgc cctgagccag ccactttata tcagtcagct tctgtgcttc ctcatcaaag
     6061 acttcaataa atgtacgccc aataattttt ctcttttcct ctggacaagt tactcccttc
     6121 aaagcagtta aaaatttatc ttcggcattg actgcaataa tgcgtattcc catatgccga
     6181 ccgaacatgc tcagcacttg ctccgcctca ttaagtctga gtaaacccgt atcaacgaaa
     6241 acacaaacca gttgctctcc aatggctcta tgaagtaatg cagctacaac agaagagtca
     6301 acgcctccgg atagtcccag taaaactttt tcagttccta cctgttctct gattttatta
     6361 atggcttcat caataatatg ctctggagtc cagtctgttg gtgctttaca aatatctacg
     6421 acaaaacgtt gtaaaattct taagccttgc aaggtatgag taacttctgg atgaaattgc
     6481 aagccataca tttgacgact ttcatcagcc atacctgcaa ttggtgcatt acgtgtttca
     6541 catatcactt taaaacctgg cggtaactct gtgactttat caccatggct catccataca
     6601 tccagtagag cactgccatc caccgcggta cgatcttcga tattacttaa taattgacta
     6661 tggccatgca aacgcaactc agcatatcca aattctctta acgccgatga atgcacttgg
     6721 ccaccgagtt gtacagccat ggtttgcatc ccatagcata tacccagcaa tggcaaatcg
     6781 ctctcaaaca gccattgagg agctctggga ttagcatcat gcgttactgt ggaaggacct
     6841 cctgataaaa tcacaccaca aggatttaat tttttaaatt gttcatgatt aatatgataa
     6901 ggataaattt cacaataaac ccccatttct cgaacgcgtc ttgcaattaa ttgggtgtat
     6961 tgagaaccaa aatctaatat taataatggg ctttttttca aatcattcat tattaattat
     7021 ccacctgata attgggggct tgcttagtaa tactcacatc atgcacatgt gactcacgca
     7081 taccagcatt ggtcacttgc acaaattcag ttttagaatg caactcctca atagtagcac
     7141 aaccggtata gcccatacag gagcgtaaac cacctaataa ttgatgaata attgtttgaa
     7201 ccggtccttt ataaggtact cttccttcaa tgccttcagg aaccagcttt tctgtaccaa
     7261 gagaagcatc ctgaaaatag cggtcacttg acccctgagc taatgacatg gctccaatgg
     7321 atcccatacc tctgtaattt ttataagtac gcccctgata taattcaatt tcaccaggag
     7381 attcttccgt acctgcaaac atactgccaa gcattacagt atcagcacct gcggccaacg
     7441 ctttgcaaac gtcacctgaa aaacgtatcc cgccatcagc tatcacagga attatccctt
     7501 taagttcttg tgccacattt gctatagcgc taatttgcgg gataccaaca ccggtcacta
     7561 tgcgagtggt acaaattgat cctggcccaa ttccaacttt tactgcatca gcacccgcct
     7621 catataaatc tcttgcggcg gcagcggttg caatattacc acctatgact tgtacatcag
     7681 gataattctt tttaatccat ttcacgcgat ttaatacgcc ctgagaatgt ccatgtgctg
     7741 tatccactac tatgacatca actccggatt caactagagc tgcaactcgc tcatcggtcc
     7801 cttcaccaac accaacagca gccccaactc ttagctgctc aaaactgtct ttacaagcat
     7861 aagggttgtc ttttgctttt tgtatgtctt ttactgtaat gagtccacgt aactcaaaat
     7921 tctcattcac taccaataat ttttctattc ggtgcttatg gagcaaactt ctgacttctt
     7981 ctctactcgc tccttctttt acagtaacca aacgcccttt gggagtcatt acttgcgcca
     8041 cagtcaaatt catattggtt tcaaaacgaa tatctcgact ggttactatt cctaccaaat
     8101 gcttaccatc aaccaccggc acccctgaaa aattatattt tgtcatgact gcaagcaatt
     8161 ctttaactgt caagtcggga gttactgaaa taggatcttt caccataccg ctttcaaatt
     8221 tttttactct tctgacttcc tctgcttgat cagcgatact catattttta tgaataatcc
     8281 ccaaaccacc ttcctgtgct aaagcgatag ctaacctcgc ttcggtcact gtatccattg
     8341 ctgccgaaat taaaggcata ttaaggtgga ttgcacgggt taatttggtt ttcaatgaaa
     8401 cgtctttggg taaaatagtt gagtgggcag ggacaagcaa aacatcgtca aaggttaatg
     8461 actgttgcac aatggataga ggcattttta tctcactcca gtaattaaat aaccaaatag
     8521 tttactacaa actt
//
LOCUS       DASLOE010000128         8476 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.129, whole genome shotgun sequence.
ACCESSION   DASLOE010000128 DASLOE010000000
VERSION     DASLOE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.129"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            332..742
                     /locus_tag="U6309_002233"
     CDS             332..742
                     /locus_tag="U6309_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018576218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN5505670.1"
                     /translation="MSRVSLRSKIEKKIGRSKRQVFLRSDFEKLSDYDQVGRALKELS
                     REGKLLKIGYGLYAKARINRITGKPMLAAAGGFTAISKEALTRLGVKWQYSDATKSYL
                     TNSTQILANAEVVVKSRFNRKIGTDKFTLKVVSP"
     gene            742..1680
                     /locus_tag="U6309_002234"
     CDS             742..1680
                     /locus_tag="U6309_002234"
                     /inference="COORDINATES: protein motif:HMM:NF020419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEN5505671.1"
                     /translation="MEEIDPTIFADVADALGIEEPVLVEKDYYAIQLLKLLHSINDPG
                     YSIIFAGGTCLSKAHIDIFRMSEDVDIKLIPSSDVQKETRGQQRKLRGHFHQKLYSLL
                     DAHTILELRKKSKRDEGKYLQCHIKYPRFHTTIDAIRPEILLEITESSLLDSTITAPI
                     YSMYSKTLKLPPEIPQCYCATIEVTASEKLVALLRRTAAYERNPSGKDDETLVRHIYD
                     LHLINRSNADKDKISKLIKKVIEIDIKDFGNQHHQFRDDPYKELLYGFEQIQNQEKFR
                     ERYQKFIGPLVYNKNPASWEESMTSLNQIVTSLMNE"
     gene            2058..3863
                     /locus_tag="U6309_002235"
     CDS             2058..3863
                     /locus_tag="U6309_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505672.1"
                     /translation="MGIFLETKRLILKRVELSDLDLLVALRSDLEVMENTGYGGTQTI
                     EEVSEYLDFAIPYQEKHGMGFCLVFEKESGNFVGEAGLFHLLFDDKQSEIELGYHLHK
                     KFWGKGYGTELAKALIQWGFQHLSVNKLISTTYPDNIASQKVLNKAGFDCMSKKQLPD
                     GEELFWYEIYKNDSVELVPYDSQWPKMAEVEIKKLRKILPTNHVIDIQHVGSTAIPGM
                     LAKPIIDIQVTVDSLTAIKQTAIDVLKAYDYVYWAEDPDPEKMFFVKGMPPFGEKRTH
                     HVHIIEPKSKRGHDRIIFRDYLIAHPEVAREYEQLKIELAHEFTYDREQYTDAKTQFI
                     ASVLGKALVTDQILNPTKYKGINELLHQLLLQIQSILANEFVGMYIGGSLASDSFNSE
                     TSDIDCYIITSKMLSESMIGKIEEMHNQFYSSKLKYAKKIEASYVPQKDLLDFDPQGM
                     RPYFNEGRYYLGHYGNNYLIELFVLREKGISIAGPDIKCLIQEISTQSLKAAIQKNLH
                     EYWESNLIDFSKFKRSDYQVFTILTMCRTLYSLETGKITSKIEAAQWAMQQLDTKWKK
                     LIELAAAWEPSQKINRLEETQNFVRYVLNKSREYQ"
     gene            3976..4581
                     /locus_tag="U6309_002236"
     CDS             3976..4581
                     /locus_tag="U6309_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5505673.1"
                     /translation="MSKLNKTRVYEVYDEIINWFDDARTKNLMESEYLNLIVNAIPPK
                     SLILDLGCGTGEPIAQFFIEKGFKLTGVDGSQKMVELCRKRFPDERWIVSDMRDINLQ
                     QQFDAILAWHSFFHLDHDSQRNMFKIFESHTKPGGVLAFTSGEEEGEVWSDNGGQQLY
                     HASLSTKEYESLLKNSSFKVLVHKVCDPECGEATVWVAQKI"
     gene            complement(4777..6117)
                     /locus_tag="U6309_002237"
                     /pseudo
     CDS             complement(4777..6117)
                     /locus_tag="U6309_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            6384..7493
                     /locus_tag="U6309_002238"
     CDS             6384..7493
                     /locus_tag="U6309_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEN5505674.1"
                     /translation="MKWNWQLENWPNFTWDSDKLVAFEQSFTEGAGIIIGSSQHISQE
                     GKQNLFIDLMCTDALDSSEIEGEHLNRNSVQSSIKKELGLSAEAPRASLAERGIAKMM
                     VNLYQTISSPLTHQVLFEWHQFLMGNSHHLEDVGQYRSHEEAMQIVSGPDYDRKIHFE
                     APPSKCVPAEMEQFINWFERSSPAGSASLPTLTRAGIAHLWFESIHPFEDGNGRIGRA
                     IAEKALSQGFSKPVMTVLAKILLKKRKEYYQQLGLASKTLDLTSWLLWFANIALEAQQ
                     STYMYIDFIIKKSIILKEAEGKINPRQEKVLLRLFHAGPDGFVGGLSAKNYMSITGAP
                     IATTTRDLNDLVKKNILKRTGELKATRYFLNLDKA"
     gene            7577..8032
                     /locus_tag="U6309_002239"
     CDS             7577..8032
                     /locus_tag="U6309_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505675.1"
                     /translation="MLQFMFFDDTYLDYKARVSYSLFCAKDFNETAAFIKANRILMTS
                     TELAAYTLKPENYKYAIIARIKNSIVGIVLFTDFESEVFINYLCVSPIHRFKGIGSNL
                     LKELKAFMGQRTLSYIDTSNNYMPWMEVLDVSKKAAVINQLGSFYKRSR"
BASE COUNT         2776 a         1392 c         1604 g         2704 t
ORIGIN      
        1 ccataaatcc aatttgaaaa ttccagtaaa ctggtacttt ctatccaaat atccatgtag
       61 ctttatagcc atatttcata aagctatttc atgcaacgaa gtgattatta cgtgttttaa
      121 aaattaaagt caacaattga aaacaaattc gatgaaaatg taactaaaat aactctactg
      181 tcccacttgt accacaggtt ccctaacttt tttcaatcat aaagactcac gtacagaaat
      241 atcatatcag attaagtgtc gtatttttgt tttgaaaagt gtcgtatttt tgctataatt
      301 atgttgtaag aaactatcgg aaaatagagc catgtcacgc gtatcactta gaagtaaaat
      361 tgagaaaaag atcggccgat ctaaacggca ggttttttta cgctctgatt ttgaaaaatt
      421 aagtgattat gatcaggttg gcagagcatt aaaagaatta tccagagaag gaaagttgct
      481 caaaataggt tatggtttgt atgcaaaagc cagaataaat agaattactg gcaaacccat
      541 gcttgctgct gcgggcggtt ttacagctat ctctaaagaa gcattaacaa gacttggtgt
      601 aaaatggcaa tattccgatg caacgaaatc ttatctgact aactctacac aaatactcgc
      661 aaatgctgag gttgttgtaa aaagcaggtt taaccgtaaa attggcacag ataaatttac
      721 cctaaaggtt gttagtcctt aatggaagaa atagacccta cgatttttgc agatgttgcc
      781 gatgctcttg gtatagaaga accagttctc gtagaaaagg actactatgc tattcaacta
      841 ctaaagttac ttcattcaat caacgaccct ggatattcaa ttatttttgc aggagggact
      901 tgcttatcta aggctcacat agacatattc cgtatgtctg aagatgtgga tatcaagtta
      961 ataccatcct cggatgttca aaaagaaacg agaggacagc aacgaaaatt acgtggtcac
     1021 tttcaccaaa aactttattc attgttagac gcacacacaa tacttgagtt gagaaagaaa
     1081 agtaagcgcg atgagggcaa atatttacag tgccatataa aatatcctcg ttttcacacc
     1141 accatagatg ctattcgtcc cgagatccta ttggaaataa cagaatcatc cctacttgat
     1201 tcaacaatta cagcaccaat ttattcaatg tattccaaaa cactcaaatt acctccagaa
     1261 attcctcaat gctattgtgc aacaattgaa gttactgcca gtgaaaaact cgtagcctta
     1321 ctcagaagaa ctgctgctta tgagagaaat ccttcaggaa aggatgatga aacattggtt
     1381 cgacatattt atgatctcca tcttataaat cgctcaaatg ctgataagga caaaataagt
     1441 aaattaatca aaaaagtcat tgaaatagat attaaagatt ttggtaatca gcaccatcaa
     1501 tttagagatg atccgtataa agagctttta tatggttttg aacaaattca aaatcaagag
     1561 aagttcagag agagatatca aaagttcatt ggtcctctgg tatacaataa aaatcctgcc
     1621 agctgggagg aatctatgac atcactcaat cagattgtta ctagtttgat gaatgagtag
     1681 aaatgccagt aaataatgtc ccaccagtcc catcgtaaat tttaaacgtg gaacgttgat
     1741 agattgatag aattaggttg tcccctaagt accacctgta ccatttaaaa taatagacag
     1801 aagcgttcag cagaagcgtt cagggtcaga tcttgccttt tgcctatcaa agtagtttta
     1861 gggaacttta gagaaagcaa aaagcaagat ccgaccctga tcctgttctg atcctgtttc
     1921 cagaagttat ccctgattta aaattaaata caatattttc gtgctacccc ggtactacct
     1981 ttcgtagcaa ctgctatcgt agaagcatca aataaaaagt ataattcctt ccagtttaaa
     2041 atatagatag acacccaatg ggcatttttc tagaaactaa aagactaata cttaaaagag
     2101 ttgaattatc cgatcttgat ctgctagtag cattgagatc tgatttagaa gtaatggaaa
     2161 atactggtta tgggggtact cagacaatag aagaggtaag tgaatactta gattttgcta
     2221 ttccttatca agaaaagcat ggaatgggat tttgtctggt atttgaaaaa gaaagcggaa
     2281 actttgttgg cgaagcgggt ttattccatt tgttatttga tgataagcaa tccgaaattg
     2341 aacttggtta ccacttgcat aaaaagtttt gggggaaagg ctatggcact gaattagcaa
     2401 aggcactaat tcagtggggg tttcagcact tatctgtcaa caagctcatt tctaccacct
     2461 atccagataa tatcgcttct caaaaagtct taaacaaagc gggctttgat tgtatgagta
     2521 aaaaacaatt gcctgatggc gaggaattgt tttggtatga aatttacaag aatgattccg
     2581 ttgaattagt gccttatgat tctcaatggc caaagatggc tgaagtggaa attaaaaagc
     2641 tacgaaaaat cctgccaact aatcatgtta ttgatatcca acatgttggt agcacagcta
     2701 ttccaggaat gctggctaag cctattattg atattcaagt tactgttgat tctctaactg
     2761 ctataaaaca gacagcgata gacgtattaa aagcttatga ctacgtatat tgggccgaag
     2821 atcctgatcc tgaaaaaatg ttttttgtaa aagggatgcc accatttggt gaaaaacgaa
     2881 cacaccatgt gcatattatt gagcccaaat caaagcgtgg gcacgataga atcattttta
     2941 gggattattt gattgctcat cctgaggtag cgcgtgagta tgaacaatta aaaatagaat
     3001 tggcacatga atttacttat gatcgggagc agtataccga tgctaaaaca caattcattg
     3061 ctagtgtatt aggcaaagct ctagtaactg atcaaattct gaacccgaca aaatataaag
     3121 gaataaatga actactacac cagctcctct tacaaataca atctattcta gcaaatgaat
     3181 ttgtaggtat gtacattgga ggttctctcg caagtgattc attcaatagc gaaacaagtg
     3241 atattgattg ctacattatt acaagcaaga tgctttctga gagtatgatc ggaaaaattg
     3301 aggaaatgca caatcaattt tattcaagta aattaaaata tgcgaaaaaa attgaagctt
     3361 cctacgttcc tcaaaaagat ctcttagatt ttgatcccca aggcatgcgc ccttatttta
     3421 atgaaggtcg ctattacctg gggcattatg gcaataatta tttaatcgaa ctatttgtac
     3481 taagagaaaa aggaatttca atagcagggc ccgatataaa atgtttaatc caagaaatat
     3541 ccactcaaag tttgaaggct gctatacaga aaaatttgca cgaatattgg gagtctaact
     3601 taattgattt ttcaaaattt aaacgcagtg actatcaggt gtttacaatt ttaaccatgt
     3661 gccgcaccct ttattcttta gaaacaggca aaataacttc taaaatagaa gcagcacaat
     3721 gggcaatgca gcagcttgat actaaatgga aaaagttgat tgaattagct gccgcttggg
     3781 agccaagcca gaaaataaat agattagagg aaacgcagaa ttttgtcagg tatgtgctga
     3841 ataaaagtcg cgagtatcaa tgagcagatc acattacttt tcagaataaa tgaagactga
     3901 aaattttttg tagtagtctg ataaaatctc acccatcaat aaatccagct attgttattt
     3961 taggagcaat tgagcatgag taaattaaat aaaactagag tctatgaagt atatgatgaa
     4021 atcattaatt ggtttgatga tgctcgcact aaaaacctca tggaatcgga atatcttaat
     4081 ttaattgtga atgcaattcc tccgaagagc ttaattttgg atttgggctg tggaactgga
     4141 gagcctatag cacaattttt tattgaaaaa ggctttaaac ttactggtgt tgatggcagt
     4201 caaaaaatgg ttgagctttg tagaaagcgt tttccagatg aacgctggat agtctcagat
     4261 atgcgcgata taaatttaca acaacaattc gatgcaatac ttgcatggca tagctttttt
     4321 catctggatc atgacagcca acgtaatatg ttcaaaatat ttgaatccca taccaaacct
     4381 ggaggagtgc tagcatttac ttctggagaa gaagaaggcg aagtctggag tgataatgga
     4441 ggccaacaac tttatcatgc atctttgtcg acaaaagaat atgaaagcct tctcaaaaat
     4501 tcatcattta aagtgctggt acataaagtc tgtgatcctg agtgtggcga ggctacagtt
     4561 tgggtagcgc aaaagattta aaatccttcg ttataaacca aaattatcag tggaattacc
     4621 tatgaaccct aattttaaaa taagatttgc aatactgtct gatgttcctt taattttgta
     4681 atttatcgct aaagggtcag atcctgatcc ttctgttgtt gcgtcactgc tggaacttgc
     4741 agtgacgcag ataagttttg caattaatta attcaattat ttgcctttat aacgaatatc
     4801 gttttcattt ccattatctt ttgcccattg agttattttt tcaaaaaggc atttattata
     4861 gttttcttgg tcggtcagat ttgcatctga aaatggttca tattgatcac tatttattag
     4921 ttcttcaagt aactcttgta cacgattaca caagctggta tctgtggtaa tatttctata
     4981 tgcatttaag ggtttgtctt ttgattcctc gtcataccct aaaaatcttt caaaatctgg
     5041 gaaacttact ctatgtcgta ttgtaaggcc ggtaccacgt gcagatatta tagaacttcg
     5101 aatttttcca ttttcggtcc acattccatt tttatttcct gattttgtat aaggtgaatc
     5161 acaatcatgg agaataccaa aatcaatttt gaaatgtttc agtactttaa ttaaaggaac
     5221 cagaatagcc ttccctcggg ctcgtattat tgtgactctt tccattagct catgtttttt
     5281 ctctaatatt gcagcaataa atgcggcgtg ttctgtatcg ccttccacta gtatggggta
     5341 tgagccaaag aaaatttcgg caaaactggg atcaatgtgc tgtaatgctt gcagtcgttg
     5401 tttttcatct tcctcaaaag taatcgtgtc ggagcgatag attttgggtt cccacgatga
     5461 ttcttgattt ggcctatcaa gtcggacaat agttgtatga tcctcaaagg ggttaatgaa
     5521 atacggtgaa tgtgtagtta tcattacttg ccaatcagga tcttcagcaa ggctatataa
     5581 atgttgttgt gcagctcgag ctgccattgg atgtaatgta ttttccggtt catcaatcaa
     5641 taaaagataa cctggtaatg caggatcgtc tatctcattt ggtagcggat cattgttttc
     5701 aagagaagtt attcgttctt gtaattcatt tatttcttta gaatcaattg gtgctttttt
     5761 ctttgtcttt gtaagcttgg tagttaattc cttaatctgt ttctcacgag actctttctc
     5821 acgtgttaat tcattatgta cttgtagcat agcccaaaac agtgctcgac gtgttcctgt
     5881 accttgttga tttaatgatg tatctgcatt accgtcattt atacgtaatc tagagccttt
     5941 tttaattaaa tcaggaacat ttggagttaa gggggcagtt gcaatgttta atctcacatg
     6001 tagatttggg aaaacatttt gaaatccact agtaattcgc tccgcaattt tattgaaatg
     6061 ctcctgatgt gaagtactta aagcatttat ttgttttgtt acatttgtta aagcttttgg
     6121 atcgttcagg gtcagatctt gccttttgcc tatcaaagta gttttaggga attttagaga
     6181 aagcaaaaag caagatctga ctctgatgaa tcctgaatca aattaatgct taaaatggta
     6241 gtctccacat tacaaactag cgcaaaaagc ctttttaatt aattcactaa acatataaat
     6301 aaattaatgc tgatgaataa cctcatattt tattaatcac taatatgcta tattttgatt
     6361 aataaaatat gaggttattc atcatgaaat ggaattggca gcttgagaat tggcctaatt
     6421 ttacgtggga ttccgataaa ttagttgcgt ttgaacagtc ttttactgag ggtgccggta
     6481 ttattattgg ttcttcgcaa catatttctc aagaaggcaa gcaaaattta tttatcgatt
     6541 taatgtgtac tgatgctttg gatagctctg aaattgaagg agagcattta aacagaaaca
     6601 gtgttcaatc ttctataaaa aaagagttgg gcttgtctgc agaagctcct agagccagct
     6661 tggcggagcg tgggatcgct aaaatgatgg ttaatttata tcaaacgatt tctagcccac
     6721 taacacatca ggttttattt gaatggcatc aatttttaat gggtaatagc catcatttgg
     6781 aagatgttgg tcaatatcgt agtcatgaag aagcaatgca aattgtttct ggacctgatt
     6841 atgatcgaaa aatccatttt gaagcgcctc cttcaaaatg tgttccagct gaaatggagc
     6901 aatttattaa ttggtttgaa aggagttctc cagccggctc agcgtcttta cccacattaa
     6961 cccgagcagg aatagcccat ttatggtttg agagtattca tccttttgaa gatgggaatg
     7021 gaagaatagg gcgggctata gcagaaaaag ccttgtctca aggattttca aaaccggtaa
     7081 tgacggtatt ggcaaaaata ttattaaaaa agagaaagga atattatcag caattaggtt
     7141 tagcaagtaa aaccttagac ctgacatcct ggcttttatg gtttgctaac attgctctgg
     7201 aggctcaaca aagtacttat atgtatattg attttattat taagaaatct attattttaa
     7261 aggaagccga agggaaaatt aatccaaggc aagaaaaggt gttattaaga ttatttcatg
     7321 ctgggcctga tgggtttgta ggtggtttaa gtgctaaaaa ttatatgagt ataacaggcg
     7381 caccgatcgc tacaacaacc agagatctga atgacttggt taaaaaaaat attctcaaaa
     7441 gaactggtga gcttaaagcg acgcgttatt ttttaaatct tgataaggct taaatggagt
     7501 ttgtgagatt ttgtacgcag tgtgtactgt acatttggcg agttttgtat ttaaatgcat
     7561 gccatctaga tcgttaatgt tgcaatttat gttttttgat gacacttatt tggattacaa
     7621 agcaagagtt agttactctt tgttttgcgc taaagatttt aatgaaacag ctgcttttat
     7681 caaagctaat cgaattttaa tgactagcac cgagttagcc gcctatacgc taaaaccaga
     7741 aaattataaa tatgccatta ttgctagaat aaagaatagc attgtgggca ttgttttatt
     7801 cacggatttt gaatcggaag tattcattaa ttacctctgt gtaagtccta tacatcgttt
     7861 taaagggatc ggctcaaatt tattgaaaga attaaaagct tttatgggac aaagaacact
     7921 ctcttatata gatactagta ataactacat gccttggatg gaagttttgg atgtttccaa
     7981 gaaagctgca gttattaatc aattaggttc attttataaa cgtagtaggt gacagccaaa
     8041 gtagtcttac tgtccattat aaaaacctat tcaactttac catcaagaga tttaattgtt
     8101 tgtaaattca tttgtataat gttttggatg gctattccaa ttttaaacaa aatttcttgc
     8161 cttgattgaa gctttctttt gtaagtttct atatttttct tcattcatgt aagaatcatt
     8221 gcgtgtagtg aagagccgta ggtgattcac aagattttgg cgctcatgaa tcagcagcaa
     8281 aaatcgcagg catcaattaa agcatgatta gttatttgtg taccaccaac caaggtagtt
     8341 agccaaatga cactataggc aaagagtaaa aggccatgaa attattatga ggcttattgt
     8401 cgacccggat ttataagtgg tcacaaatag tacataaaaa tgaagtggtg tcaggatggc
     8461 ataggaattg attcat
//
LOCUS       DASLOE010000129         8331 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.130, whole genome shotgun sequence.
ACCESSION   DASLOE010000129 DASLOE010000000
VERSION     DASLOE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.130"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..558
                     /locus_tag="U6309_002240"
     CDS             <1..558
                     /locus_tag="U6309_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEN5505676.1"
                     /translation="ASHQELQGEVTYQRILQVLGERAFGIVLLFFALPSALPFSFIPG
                     ISLIFSVPILLFAFQMVFARKTMWLPKIIAERTIHQETMAKFIHNTVPYLIKIEYFLK
                     PRWLFMTSRFMEIIHGIVIFFLALLLMLPIPFSNFIFATLLIIFSLGLIEKDGLFLII
                     GYLGVVIYANFVYLFVLTAVKHLFG"
     gene            1309..2277
                     /gene="lem4"
                     /locus_tag="U6309_002241"
     CDS             1309..2277
                     /gene="lem4"
                     /locus_tag="U6309_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem4/SmdA"
                     /protein_id="HEN5505677.1"
                     /translation="MTTSVSFGETSKRNEGRVKNIVYLDFDGTITGAHGKEVISSPLC
                     EALSTKATFDERMRYKNEYDASENKIKITENAKKFLQDVNKLHPQVKIVIISRNHENY
                     IKALLEFENIDHRNITIYPRGVGNTIGPGEDKYKAVVSHEEKPECLPGFRLICDDDEV
                     DGEEMCNGLIHTGRSQLVKYHNEKPGQFKWGEYFKEILTNCDIAVKEYLNGKIGSRFH
                     LFTAKVDEKTGDQSFKDRYSQVKGDILKRAIINNLKNDIEKIDNLDDLKDFIKKFKQS
                     SEYMTLSKAQGLFTKVTGIKTDSQRAVEEIFSKALKDLQSPKLAMK"
     gene            complement(2563..3024)
                     /locus_tag="U6309_002242"
                     /pseudo
     CDS             complement(2563..3024)
                     /locus_tag="U6309_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3330..4541
                     /locus_tag="U6309_002243"
     CDS             3330..4541
                     /locus_tag="U6309_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN5505678.1"
                     /translation="MVMKTMRLLKGILFTFCGIIFCFNSHAALELEKLVNYISLGEFS
                     RETAEIALKKMPPLDTLSVHYDLQLYKINYKTQTPDGNLTIASGLVAMPIHPMGQVGI
                     VSYQHGTRFERNDVPSRNNEKNYIYLAAYGNSAGYMTVMPDYLGLGDNELTLHPYVQA
                     ETLASSSIDMLLAAKELANRLHYPISDKLYLAGYSEGGFSTIVMFEMLAKEHPDIPVS
                     AVAPGSAPYGWEETMHFVMMEPGPRATAYLAYFFYSLQTYKSYWSGFDEIFVPPYNTL
                     IPELMDGYHTVAEILQALPPNPLLIFQPKFSNGIISKTDRNTEILKINFNHYDFKPTA
                     PLLLVGTRGDRDVPYAGAEMAYHSFRKYSDFVWIKSVSDTLDHVQAHPFVLKEQVDFF
                     KQFERQEVMNK"
     gene            complement(4585..5577)
                     /locus_tag="U6309_002244"
     CDS             complement(4585..5577)
                     /locus_tag="U6309_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEN5505679.1"
                     /translation="MNQKQIKNQLALKWALEHLALNDEFKIIDQQKIIETAYSTVHRI
                     TSSESNVYYLKQTPEMLYLEPQTLTYLKEQRCHNIPQIIAENKHLNCFLMLSCGDQTL
                     RNLFKENIDLEMLKAGILNYTKIQRLVENQLPQLIKLGIPDWRLEKFSSLYDQLIQQE
                     DLLLNDGLSKNEITLLNKAHEICFNLCESLSKYQIPETINHCDFHENNMILNQKTGNV
                     SIIDWGETVITHPFFSLNGCLWNITFFYPIKQSDKIYEALQRQCITSWLGLHDETDLL
                     NALKIANQLNGFFAALSYKRIYDATMDQYQTVQQEHPGSIAGCLRSFLNYFHLA"
     gene            5775..6278
                     /gene="msrB"
                     /locus_tag="U6309_002245"
     CDS             5775..6278
                     /gene="msrB"
                     /locus_tag="U6309_002245"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN5505680.1"
                     /translation="MTKLISVVFYLFIIPNVMAYTPQFNKSEQLKKLTPLQYQVTQNG
                     ATEKSFDNPYWNNKEEGIYVDIVSGEPLFSSNDKYDSGTGWPSFTKPIDTSYLVFHTD
                     RSYFFIVRTEVRSKYANSHLGHVFKDGPEPTGLRYCMNSAALKFIPKNEMEKAGYGEY
                     LYLFNKK"
     gene            complement(6377..7639)
                     /locus_tag="U6309_002246"
     CDS             complement(6377..7639)
                     /locus_tag="U6309_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505681.1"
                     /translation="MRNNKMGEKIPSFFHHGNKATSPQILVDAPLIIKDSELLDLLNS
                     PIDKDKKYYRLISIIRSHSPEYLNQRIMVKSHRTIEGDERIPPHWTSLATKREYAYPT
                     DENPSNELTTTLANIIATNELNFSSAQMVALFRELMIKEVDFTAIDQSPYLNLIHPKS
                     GNNPLKSLIAASLGNPEIIHLLYDFISHIENKIPGDQQLLIINNQDNFEFEAMQPTEF
                     LLRLGHEDLALRLFKMGARPTVQAFRLACSSYGTTPHYEETMECIQFLMNSVELHEME
                     LLEGKSSLQKASPAQYNEMLTRILHSLQDDSKLDTGHSSLFEMIQKDYIENYQDKMLI
                     SFRDFCQKEAKIDHPLIQKYALLRIKQENIGNGLFSDYYRTESRHLAEKKWTLLNKNQ
                     RANQLTHDLIDLIDTKLGSLSMFPSARL"
     gene            complement(7885..8304)
                     /locus_tag="U6309_002247"
     CDS             complement(7885..8304)
                     /locus_tag="U6309_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEN5505682.1"
                     /translation="MNFSAWYFPSLAALILYGAWGYWGTRASDFINPLSITFYSSIGV
                     LISGIIALILLGFKPELSVKGSTYGLLNGLANGIACIFFILALRNGPTMPVVLVTSMY
                     PMITLIFCMIFLKQELSLKQGLGMVFALIALVLFSTE"
BASE COUNT         2569 a         1406 c         1548 g         2808 t
ORIGIN      
        1 gcaagtcatc aggagttaca gggggaagtt acttatcaac gtattttgca agtgcttgga
       61 gagcgtgcct ttggaattgt gcttttattt tttgcgttgc ctagtgcgct gcctttctca
      121 tttattcctg gtatctccct gatttttagt gttccaatat tattatttgc atttcaaatg
      181 gtttttgcca gaaaaacaat gtggctacca aaaattattg cggaacgaac cattcaccag
      241 gaaacaatgg ccaagtttat acataatacc gttccttact taattaaaat tgaatatttt
      301 ttaaaaccca gatggttgtt tatgacttcc cgctttatgg aaataattca tggaattgtt
      361 attttcttcc ttgccctatt attgatgtta ccaattccat ttagtaattt tatttttgct
      421 acgttgttaa ttatttttag cttgggatta atagaaaaag atggattatt tctcattata
      481 gggtatctag gggttgtgat ttatgcaaac tttgtctatc tgtttgtctt gacggcggtg
      541 aagcatttgt ttggttgatt gaaagcttgt gttactaacc attagggttc agctttttca
      601 ttatagttga attaactata tgtaaataaa taaaaaattc cacttaaaat cactcggaat
      661 aactttgatg cagtaatttt ttttcaggtt ctcacataac cgaattaaga tgcctgctat
      721 cctggagtct cagcatataa tggaatgcct ggctaacatc aagtattaag catgatacag
      781 acggagtaag atgatatggg tagatggtct ctgttttttg cagcaaatcg aaatagtaaa
      841 ccccataaaa tagttgagga agcttatgtt ttcgttggta gttctttttt cttttagtgg
      901 agccccaagc tttcaggatc ttcttatatg cattctccgc agaggtcttg ctttttgtca
      961 atctcagata gcaggcaaaa agcaaggcct gttatacgaa ccacagatat gttatcaatc
     1021 ttacctgggg gagttttcac tgcttcataa tggttaataa aataaattgc catgtacttt
     1081 agtttattaa cctcatggtg ttcgttcttt taatttgcta ctgctgatta attttttctt
     1141 tcaatacaac ccatatttat ctgaacgcaa gcgcacaaat attcattaag ccattgaaaa
     1201 acaatagata ttattaaaaa taatatgact tatgaaaaca attaattgta cacaaaacaa
     1261 gcataatgat taaatgtatg cattgggtgg ttctacgagg aaataatcat gactacgtcc
     1321 gtgtcttttg gtgaaacttc aaaaagaaat gaaggcaggg taaagaacat cgtttatctg
     1381 gatttcgatg gaacaataac tggtgcacat ggcaaagaag tgattagttc ccccctgtgt
     1441 gaagctttga gcactaaagc gacttttgat gaaaggatgc gctataaaaa tgagtatgat
     1501 gcatctgaga ataagattaa aataacagaa aatgccaaga aatttctaca agatgtcaat
     1561 aaattacatc cacaggtaaa aattgtaata atcagccgta atcatgaaaa ttatattaaa
     1621 gctcttttag aattcgaaaa tattgatcat cgaaatatta ctatttatcc tcgcggtgta
     1681 ggaaatacaa tagggccagg ggaagacaag tacaaagcag tggtatcaca cgaagaaaaa
     1741 cctgaatgtt taccaggatt tcgtttgatt tgtgatgatg atgaggtaga tggggaggag
     1801 atgtgtaatg ggcttataca tactggacgc tcccaacttg taaaatacca taacgaaaag
     1861 ccaggccaat ttaaatgggg tgaatatttt aaggaaatat taactaattg cgatattgct
     1921 gtaaaagaat acctgaacgg aaaaataggt tcacgcttcc atctttttac agcaaaggta
     1981 gatgaaaaaa caggtgacca atctttcaaa gatagataca gtcaagtaaa aggagatata
     2041 ttaaaacgcg caataattaa taatttaaaa aatgatatag aaaaaataga taatttagat
     2101 gatttaaaag actttataaa gaaatttaaa caaagctccg aatatatgac ccttagcaaa
     2161 gcccaagggt tatttactaa agtaaccggg ataaaaactg attcgcaacg ggcagtagaa
     2221 gagattttta gtaaggcact caaggattta caatcaccca aacttgcaat gaagtagctc
     2281 aaatttgatc gggcagttgc catttatgag ctgactgcct gatcaaattc aatttaaatt
     2341 tttcaatttg gcgcagactg tagcagtaca agttgttctc atagccatgt ctctgttcta
     2401 ccagtagtat ttaaataact gtgttttgtt tgtcttgata gcggtgcggc attttcttga
     2461 ttggttgata aaaagcttat gttactaacc attaagcttc atcactttag caagatttcc
     2521 agttgtttgg ctccaagtgg ttaagctcat tagtacatag gcttatatag gttctttttt
     2581 cggttttatt gaaaatgggt ttggagtagt gtaagtgttt tcttgtggag ttattcctgc
     2641 ttcagtgaag tctttttcct ttttgggtga tgctaaatta ggtttcataa aaatttcatt
     2701 ttcaattctt ctcaaactag ttctcatctg ctcattatcc ggatcctttt ttaagtcaaa
     2761 ctctaattgc atacggctct ttgataatga acctttatac aaacgcccat taccacaaga
     2821 tagacaagca tcctcttttt cagctgagag tgctgtcctg tcggcaggtt ttcttcttgc
     2881 tcgttgttcg caagcatata acatattact gaattctcgg atataatcat ccacagcact
     2941 tctgatgcat ttttcttcct gtactttatc ttcttctaac tttttcttag cttgctcttc
     3001 ttatctggac attgtgatta ccataaatta tttgaacatc aattcactgt ggaattgaaa
     3061 ataaaattat cttataaaat taatgatagt atatttctct ggagttactt atctctctga
     3121 attttcctat aaaattcata cattttttca gcaacagggg tatttgtaga taatacttca
     3181 ttcatttcat cgggcatttt aattgaaatc tcgtttgtta gatgattgga ataaatagta
     3241 aggaactgat tgtatccttt gcattaatgc aagtaaccgc ttcgtttgat tctccttatt
     3301 aaacatgtaa tcatctgcat tttttataga tggttatgaa aacaatgcgt ttattgaaag
     3361 ggattctttt tacattttgt gggattattt tttgctttaa ttctcatgct gctctggagc
     3421 tcgagaagct ggttaattat atttctttgg gtgaattttc cagggaaaca gcagaaatcg
     3481 ctttaaaaaa aatgccgcca ttagacaccc tttcagtaca ttatgatctg caattatata
     3541 aaattaatta taaaacccag acaccagatg gaaatctgac tattgcttca ggattagttg
     3601 caatgccaat tcatcctatg gggcaagtgg gaattgttag ctatcagcat ggcacccgat
     3661 ttgaacgcaa tgatgtacct tccagaaata atgaaaaaaa ctatatatac cttgctgcct
     3721 atggtaatag tgcaggttat atgacggtga tgccagatta tcttggtctg ggcgataatg
     3781 aattaacact tcatccttat gttcaggcag aaactcttgc tagcagtagc attgatatgt
     3841 tgcttgctgc taaggagtta gccaacaggc tgcattaccc aataagtgat aagttatatc
     3901 tggcaggcta ttcagagggg ggattctcaa caattgttat gtttgaaatg ctggcaaaag
     3961 agcatcctga tatacctgtt tctgcggttg ccccaggttc agccccttat ggttgggaag
     4021 aaaccatgca ttttgtgatg atggaaccag gaccgcgagc tacagcctat ttggcctatt
     4081 tcttttattc tctgcaaacc tataagagtt attggtctgg ttttgatgaa atttttgttc
     4141 caccttacaa tactcttatt ccagaattga tggatggata tcatactgtt gccgaaattc
     4201 ttcaagcctt accaccaaat cctctgctta tttttcaacc caagttctct aatggaatta
     4261 ttagcaagac agatagaaac actgagattt taaaaattaa ttttaaccat tatgatttta
     4321 aacctacagc tcctttgtta ttggttggta ctagaggtga tcgtgatgta ccttacgcag
     4381 gagctgaaat ggcatatcat tcctttagga aatatagtga ttttgtatgg ataaaatccg
     4441 tcagtgacac cttggatcat gtacaggccc atccctttgt actcaaagaa caggtggatt
     4501 tttttaaaca atttgagcgt caagaagtga tgaataagta atatcacctt gtgaaactca
     4561 gcaatcacag ttttgatgta acagtcaagc taaatgaaaa tagtttaaaa atgatcttag
     4621 gcagccggct atggagccag ggtgttcttg ttgaactgtt tgatattgat ccatagttgc
     4681 gtcataaatt cgcttataac ttaatgcagc aaaaaaccca ttgagttggt tggcaatttt
     4741 tagcgcattt aataaatcag tttcgtcatg cagacctaac caagaagtaa tacattgtcg
     4801 ttgtaatgct tcatatattt tgtcggactg ttttattggg taaaagaaag ttatgttcca
     4861 caagcagcca ttcagagaaa aaaatggatg agtgatcacg gtttctcccc agtcaatgat
     4921 gcttacattt cccgtctttt gattgagaat catgttattt tcatgaaaat cacaatgatt
     4981 aattgtttct ggaatttggt atttggagag tgattcacat aaattaaagc aaatctcgtg
     5041 tgctttgttc aggagggtaa tctcattttt tgatagccca tcatttaata aaagatcttc
     5101 ttgttgtatc agctggtcat atagtgatga aaatttttca agccgccaat cggggatacc
     5161 caattttatg agttgtggga gttgattttc tactaaccgt tgaattttag tgtaattaag
     5221 aatacctgct ttaagcattt ccaaatcaat attttcttta aacagattcc gtaaagtttg
     5281 atcgccacaa gacagcatca aaaaacaatt taagtgttta ttttctgcaa tgatttgggg
     5341 aatgttgtga catcgttgtt ctttaagata ggttaatgtt tggggttcca ggtacaacat
     5401 ttccggtgtt tgcttcagat aataaacatt gctttcggaa gaggtgattc tatgtactgt
     5461 tgaataagcg gtttcaataa ttttctgttg atcaatgatt ttaaattcat cgtttaatgc
     5521 cagatgctca agagcccatt ttaaggcgag ctgatttttt atttgctttt gattcacagt
     5581 ctgctcataa ctaataatct ttaatatgaa tttaatgtat aaaatagttt aagtccaaac
     5641 atcatgctta tcttctgttt tgatccttga tggaaacgca gatttattac tctcttcgct
     5701 aatatggggc acgcttgcta cactataagt aagcatcatc aatgtgggca tgaaagtgca
     5761 tgtggagtta tgacatgaca aaattaatca gcgttgtatt ttacctgttt ataattccaa
     5821 atgtgatggc ctatactcct caatttaaca aatcagagca gcttaaaaag ctgactccat
     5881 tgcaatatca ggtaacccaa aatggggcaa ctgaaaaatc attcgataat ccttactgga
     5941 acaataaaga ggaaggtatt tatgttgata tagtatcagg agaacctttg tttagttcta
     6001 atgataaata cgattcagga accggttggc ccagttttac aaagccaatc gatacctcct
     6061 atttggtttt ccatactgac agaagctatt tttttattgt gcgtactgaa gttcgctcta
     6121 aatacgctaa ctctcattta ggccatgtct ttaaagacgg cccagaacca accgggttgc
     6181 gatattgcat gaattcagcc gccttgaaat ttattccaaa aaatgaaatg gaaaaagcgg
     6241 ggtacgggga gtatctttat cttttcaata aaaaataaac tgcccttgtt ttttcagcaa
     6301 gttagcctat ggaactcatt gagctcaaat caatgttaga gcttaaatgg gctgaacttg
     6361 ccgtatgttc ctcaaattat agcctggctg aagggaacat gcttaatgat ccaagtttag
     6421 tgtcaattaa atcaatgaga tcatgtgtta attggttggc tctttgattt ttattgagta
     6481 aagtccattt cttctcggcc aaatgtctgg attcagtgcg gtaataatct gagaacaagc
     6541 cattacctat gttttcttgc ttgatgcgca aaagagcata tttttgaata agtggatgat
     6601 caattttcgc ttctttctga caaaaatcac gaaaagaaat gagcatttta tcctgataat
     6661 tttctatgta gtctttctgt atcatttcaa agagagagga atgtccagta tcaagtttcg
     6721 aatcatcttg cagggagtgt aatattcttg tcagcatctc gttgtattga gcagggcttg
     6781 ctttttgcaa ggaacttttt ccctccaata attccatttc atgcaactca acagaattca
     6841 tcaaaaattg aatgcattcc atggtttctt cgtagtgagg ggtggtgcca taactggagc
     6901 aagccagtcg gaatgcttgt acagtcggtc gagctcccat cttgaataac cgtaaagcta
     6961 gatcttcatg gcctaggcgt aataaaaatt cagtcggttg catcgcctcg aattcaaaat
     7021 tatcctgatt gttgataatc aatagttgtt gatcgccagg aattttattt tcaatatgac
     7081 taatgaaatc atacagtaag tggataatct caggattacc taaactggca gctatcaaac
     7141 ttttgagagg gttattgcca gatttgggat ggattaaatt aaggtaaggt gattgatcta
     7201 tggcagtaaa atcgacttcc tttatcatta actctctaaa caaagcaacc atttgagcag
     7261 aagaaaagtt taattcattg gtagcaatga tatttgccag ggttgttgtc aactcgttac
     7321 ttggattttc atcggttgga taagcgtatt ctctttttgt tgccaaagat gtccagtggg
     7381 gaggaattcg ctcatcacct tcaatcgttc tatggctctt aaccataatt ctttgattta
     7441 aatattcggg actgtgactg cgaatgatgg atattaggcg gtaatatttc ttgtctttgt
     7501 caattggcga gtttaacagg tctaacaatt cactatcttt aatgattaat ggagcgtcta
     7561 ccaatatttg aggtgaggtg gctttattac catgatgaaa aaaacttgga atcttttctc
     7621 ccattttatt atttcgcata gttaagaacc tatttcaaaa agtttaaaag cgatagctga
     7681 tgcaacaaaa gcctcgaacc catgttttgt ttgtaataat caatagatcc ttttttactc
     7741 ctattttagt catgatgatt tcatcttgat tagattgttt atataagtca agaatatttt
     7801 catgctatct gattaaaaat tggggtcttc caatccctaa attgattcgt caaccccctg
     7861 aaatacttgg caagcaagag tttcttattc cgttgaaaat aaaactaagg caatcaaggc
     7921 gaaaaccatc cccaatcctt gttttaaact taattcttgt ttgagaaaaa tcatgcagaa
     7981 tattaaagta atcatgggat acatagaggt gaccaaaact actggcatgg tggggccatt
     8041 acgcaatgcc aaaataaaaa aaatacaggc aattccatta gccagcccat tgagcaagcc
     8101 ataagtactc cctttaacag aaagttcggg tttgaaacct aataaaatca aagcaataat
     8161 tcctgagatt aaaaccccta tacttgaata aaaggtaatg gataggggat taataaaatc
     8221 agaggctctt gttccccaat acccccaagc cccatacagt attaatgcag ccaaagaagg
     8281 gaagtaccaa gcactaaaat tcataatttt atcttatcaa gcaatttgtt t
//
LOCUS       DASLOE010000130         8276 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.131, whole genome shotgun sequence.
ACCESSION   DASLOE010000130 DASLOE010000000
VERSION     DASLOE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.131"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..582
                     /locus_tag="U6309_002248"
     CDS             <1..582
                     /locus_tag="U6309_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505683.1"
                     /translation="TSISLSGVKNDGYVGGEFCNSGNLIKEEVLEALNIHINSKNTAE
                     CSSDEEIKALAESLCLEHQNALLVPELSEKARILTESSQEQENELVTKTKQIETDKKT
                     ILEQQATIQEKDKEIEALKEKISDLTRTAPRPVFNPVLPMGYSSLFFNTRLGTQAKQQ
                     TTFDVTDLPIIKSSESKDAEPNKSFSMGNSHGD"
     gene            734..3118
                     /locus_tag="U6309_002249"
     CDS             734..3118
                     /locus_tag="U6309_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEN5505684.1"
                     /translation="MKKMAYFWRKGLWALMSLFFLLIVAGSLLYLYLESQLPNVDSLK
                     TVQLQVPLQIFSKEGLLIQEYGEKKRIPVTYDEIPPTLIHALIATEDQRFFEHPGVDV
                     MGLGRAAVSMLKTGTKSQGGSTITMQVARNFFLSRKKTFLRKFNEIMLAIKIDRELSK
                     EKILELYLNRVYLGNRAYGVGAAAMVYFGKSLKDLNLAELAMIAGLPQAPSTQNPIAN
                     PLAAKKRRDHVLERLLEEHYINEEQYQNAVNQPITAKYHGTNIKVKAPYVAEMIRQSL
                     YDNFGPEAYTKGYKVYTTIDGKLQNTANEVVEKNLIAYDHRHGYRGPIANIGEKDSQS
                     PKIRQKYLRQYPELNSLIPAVVTEVREKEATAALQNGQIIIIPWEGMSWARPALKRGG
                     WVGKSPTKAQQVVAAGDIIYVHPTEKSWQLAQIPEAESAMVAMNPKNGAIEVLVGGFN
                     FSKSKFNRATQSSRQPGSSFKPFVYAAALNNGYNLATLINDAPIVVDDPSQPNLWRPH
                     NVNLKFNGPTRLKQALVQSKNLVSIRILDDIGIDYTIDFLTRFGFNKKSLPRALSLAL
                     GSLSISPMDLTTAYAVFANGGYKVEPYLIDHITDADGKILLQAKPSVVCDPCDKDKVD
                     ASTLAPRVISEDIAFLMNTALRDVIQHGTGRAARVLNRQDIAGKTGTTNDQVDAWFAG
                     FNSNLVVTTWIGFDNPKSLHEYAANLALPLWIDFMKVALKDKPETELKQPENVIAVRI
                     DPVSGLLAKPNQENGIIEYFRQNEVPSEEDQGPVYNAHNDQQEEELAPVEESLF"
     gene            complement(3190..4311)
                     /gene="ald"
                     /locus_tag="U6309_002250"
     CDS             complement(3190..4311)
                     /gene="ald"
                     /locus_tag="U6309_002250"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEN5505685.1"
                     /translation="MLVGVPKEIKSHENRVGLVPSSVREIIRAGSDVLVEKGAGLGIG
                     ISDDDYRNAGAEVVDTADDVFANAELIVKVKEPQPIECKRLREGQTLFTYLHLAPDPH
                     QARLLKESGVTAIAYETVTQDDGGLPLLTPMSQVAGRMAIQAGAHCLEMAQGGSGILL
                     GGVPGVAAANVVVIGGGVVGTNAVRMAMGMEARVIVLDRSLQRLNELDFQFGSKLNTV
                     YSTADSIEKYVSSADLVIGAVLVPGAAAPKLVTRSMLSSMRPGSVLVDVAIDQGGCFE
                     TSRATTHQEPTYVVDNVVHYCVANMPGAVPRTSTFALNNATLPFILSIVTKGVKLALL
                     SDPHLLNGLNVHQGKITYEAVARDLGYEYTPAAAALSGR"
     gene            complement(4323..5261)
                     /locus_tag="U6309_002251"
     CDS             complement(4323..5261)
                     /locus_tag="U6309_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEN5505686.1"
                     /translation="MSTLVLVEHNNQQLHPATRNTLSAALELGDKITLLVVGYQCRSV
                     AEEAASLEGAHAVLHVDNPCYEHQLAEAVSDLVVSFANSFSAILAPSSTYGKNILPRV
                     AAQLDVTQISDVSKIINSDTFEHPVYAGNAIETVRVLDSLKVMTIRTTAFDPITSSQE
                     VCCIESIDKEFQAQGSQFVRHELSKSERPDLGSAKIVVSGGRGLQNAEKFKLIEELAD
                     TLGAAVGASRAAVDAGFVPNDYQVGQTGKVVAPMLYVAVGISGAVQHLAGMKDSKVIV
                     AINKDADAPIFQIATYGLVGDLFELVPQLIEQLKNR"
     gene            complement(5275..6024)
                     /locus_tag="U6309_002252"
     CDS             complement(5275..6024)
                     /locus_tag="U6309_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEN5505687.1"
                     /translation="MKILVAVKRVIDPYVKIRVKSDNSGVETQNIKMAMNPFDEIAIE
                     EALRLREKNWATEVIAVSIGADSSQETLRHALALGADKAILVRTEKSFESLNIAKILN
                     NIVANEKPDLVLMGKQSIDGDNNQTPQMLAALLNWPQATNASKIIPDNNNLEVVREID
                     GGLETLKVQLPAVISTDLRLNEPRYASLPNIMKAKRKPLDIIDLDSLGLSLKQHVEIL
                     KVNAPATRSGGVILDSVAALLDKLQHEAKVL"
     gene            complement(6433..7677)
                     /locus_tag="U6309_002253"
     CDS             complement(6433..7677)
                     /locus_tag="U6309_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEN5505688.1"
                     /translation="MTIERELKNFVRLYAEWDTTNNCYKSHLDKDTLLRLERDFARFI
                     NQQLLIDGPEKEKIVKNYKRLTLCFHPDHASGFSPEMVWLENNLSQSKNNGACFKILG
                     LCYEKLISPEKFKDSSLGDIKSKDDFKQWLENLRSKATTYSGQSFCDSLINLLEQSSG
                     FFDDTGKIKPRGLRVLLRFIPVVFASYGTFLFAEELFAIYALYFILLKGGQYMESTES
                     SQLKKIGCTLQEISIITATASTTLLVRLLEMTFWASRQFLDVSLQIGSAILKPMLPAP
                     ETKEKSESDTAANLCRDLILASHNTGEGMQFKTPELKVISAPLESYLGLNAQQFFGDL
                     RIGREKRLKVEAFLFKMRVLDSLPSPLEEKLVEAQKELDKIKKDTKVFTSNTAKAVKE
                     AEQVISLLQDKDSMQLVVYNGT"
     gene            complement(7717..>8276)
                     /locus_tag="U6309_002254"
     CDS             complement(7717..>8276)
                     /locus_tag="U6309_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN5505689.1"
                     /translation="YYFLDRTLATYFHSLDLRVNAHALTYLTALGKWKIYVALFLITA
                     LYFRYIQQNKQYEIRSWYLLGCVLLPNLLTFVLKISFSRARPDLLFDNNLYGFYWFQH
                     NDNYWSFPSGHSITITALAAGLGFLFPRYFFLFIGAALLVAATRVILYHHYLSDVMTG
                     FYISMLLVGLFTQYLQRNHYLNKIN"
BASE COUNT         2642 a         1754 c         1486 g         2394 t
ORIGIN      
        1 accagcattt ctctttcagg cgtaaaaaat gatggatacg ttggtggaga attttgcaac
       61 tccggaaatt tgatcaaaga agaagtttta gaagcattga acatccatat taattctaaa
      121 aatacagcag aatgttcttc tgatgaagaa attaaagcgc ttgctgaatc tttatgttta
      181 gagcatcaaa atgcgctctt ggtacctgaa ttatcggaga aggcccgaat attgactgaa
      241 agcagccaag aacaggaaaa cgaactggta acaaaaacaa aacaaattga aacagataaa
      301 aaaacaattt tagaacaaca agctacaatt caggagaaag acaaagaaat agaagcactg
      361 aaagagaaaa taagcgattt aacgcgcact gcaccccgcc ccgttttcaa tccagtctta
      421 cctatgggat acagctctct gtttttcaat accagattgg gtactcaagc taaacaacaa
      481 acaactttcg atgtaactga tttaccaata attaagtcct cagaatcaaa agacgctgag
      541 cctaacaaat cgttttctat gggtaatagc catggagatt aaagtctatt ggcgcttcat
      601 cgatgattat ctccatagct gtgaggcgca aaatagcatt taaatcaggt ttttttccct
      661 tgtaaacctc gcaatattat atagattcgc tgtataatat tgcactactc acatttaacg
      721 aaaacgaaat tctatgaaaa aaatggcata cttctggcgt aaaggtcttt gggcgctgat
      781 gagcctgttt tttctcctaa tagtggctgg cagccttcta tacctttatc tcgaaagcca
      841 attgcctaat gttgattcat taaaaactgt acaattacaa gtgccattac agattttcag
      901 caaagaaggt ttattaattc aggagtacgg tgaaaaaaaa cgtattcccg taacttatga
      961 cgaaattccc cctactctga tccatgcatt aattgcaaca gaagatcaac gattttttga
     1021 gcaccctggt gtagacgtta tgggattggg ccgagccgca gtaagcatgc tcaaaactgg
     1081 aaccaaatct caaggtggca gtacgatcac catgcaagta gctcgtaatt ttttcttaag
     1141 ccgtaaaaaa acatttttac ggaaattcaa tgaaattatg cttgctatca aaattgaccg
     1201 ggaattaagc aaagaaaaga ttttggaact ttacttaaac agggtttatc tgggtaatcg
     1261 ggcttacggt gttggcgcgg cagccatggt ttattttgga aaatccctta aagaccttaa
     1321 tctggctgaa ctggctatga tagcaggact tcctcaagct ccctcaactc agaatcctat
     1381 cgctaacccg cttgctgcaa aaaaacgtcg tgaccatgta ctggagcgat tactggaaga
     1441 acattatatc aatgaagaac aataccaaaa tgcagtcaat caaccaatta ctgcaaaata
     1501 ccatggaact aatatcaagg tgaaagcgcc ttatgttgct gaaatgattc gtcagtctct
     1561 gtacgacaat tttggccccg aagcgtacac taaaggctat aaggtatata caaccattga
     1621 tggcaaatta caaaatactg ccaatgaagt ggtagaaaaa aatctaattg cttacgatca
     1681 tcgacatggt tacagagggc caattgccaa tataggtgaa aaagacagtc aatctcccaa
     1741 gattaggcaa aaatacttgc ggcaatatcc agagctgaac agtctgattc cagcagtggt
     1801 cactgaagta agagaaaaag aagcgactgc ggcattacaa aacgggcaaa taattattat
     1861 tccctgggag ggtatgtcct gggccaggcc ggcactgaaa agaggaggct gggttgggaa
     1921 atcaccaacc aaagcacaac aagtggtagc agcaggcgat ataatttatg tacaccccac
     1981 ggaaaaatcc tggcaactcg cccaaattcc tgaagcagaa tctgcaatgg ttgctatgaa
     2041 tcctaaaaat ggcgccattg aagttttggt cggaggattt aacttctcaa aaagtaaatt
     2101 caaccgagct acccaatcca gcaggcaacc aggctccagt ttcaaaccat ttgtttacgc
     2161 tgcggctttg aacaatggtt ataatcttgc taccttaatt aatgatgccc ccatcgttgt
     2221 agacgatccc agccaaccga atttatggcg gccacacaat gtgaatctaa aattcaatgg
     2281 ccctacccgc ctgaaacagg cattagttca atctaaaaat ttggtatcta taagaattct
     2341 tgatgatata ggtattgatt atacgataga tttcttaact cgttttggct ttaataaaaa
     2401 atcattgcct agagccttat cacttgcttt aggaagtttg tccattagcc ctatggattt
     2461 aaccactgct tatgcagtct ttgctaatgg tggttataaa gtagagccct acctaattga
     2521 ccatataaca gacgctgatg gtaaaattct gttacaagcc aaaccaagtg ttgtatgtga
     2581 cccttgtgat aaagacaaag ttgatgcctc taccttggca cctcgtgtta tttctgaaga
     2641 cattgccttt ttaatgaata cggcattacg cgatgtaata cagcatggca ctggacgtgc
     2701 tgcacgagta ttaaatcgtc aagacatcgc tggtaaaaca gggactacta atgatcaagt
     2761 agatgcgtgg tttgccggat ttaactctaa tttggtagta acaacctgga ttggttttga
     2821 taatcccaaa tcattgcatg aatatgctgc aaatcttgct cttccgctat ggattgattt
     2881 tatgaaagta gcattaaaag acaaacctga aactgaattg aagcagcctg aaaatgttat
     2941 agctgttcga atagatcccg taagtggttt gctcgccaag cctaaccagg aaaatggcat
     3001 cattgagtac tttcgacaaa atgaggttcc atctgaagag gatcaaggtc ctgtctataa
     3061 tgcacataat gatcagcagg aagaagaact agcgccagtg gaagaaagtc tgttttaatt
     3121 tgagatttat attctgatga agcacaaaac ccgtattcaa tttgtgtaat acgggtttta
     3181 atccgctaat tatcgtcctg ataaagcagc tgcagctggt gtgtattcat atcctaaatc
     3241 acgagcaaca gcctcataag tgattttacc ctggtgaaca tttaggccat taagtaaatg
     3301 agggtcactt aataaagcca gcttaacccc tttagtaaca atacttaaaa tgaaaggtaa
     3361 tgtggcatta tttaaagcaa atgttgatgt tctgggtaca gcaccaggca tattggccac
     3421 acaataatgc actacgttat caaccacgta agtaggttct tgatgggttg tcgcacgact
     3481 cgtctcaaaa caccctcctt gatcaatagc aacatcgact aaaacggagc caggacgcat
     3541 tgagctcagc atagagcgag tcactaattt gggggctgca gcacctggta ccaatacagc
     3601 accaatcact aaatcagcgc tggatacgta tttttctata gaatctgcag ttgagtaaac
     3661 tgtattgagt ttagaaccaa actgaaaatc caattcattt aaacgttgca gtgaacgatc
     3721 taacacaata acacgagctt ccatccccat cgccatgcga acagcatttg ttcctacaac
     3781 tccaccgcca ataactacga cattggctgc agcaacacca ggtacaccac ctagcaatat
     3841 accgctaccc ccttgagcca tctccaagca atgtgcaccg gcctggattg ccatacgacc
     3901 agccacttgg gacatgggag taagtaaagg taacccgcca tcatcctgtg tcacagtttc
     3961 ataagctatc gctgtaaccc cggattcttt taataaacgc gcttgatgag gatcaggagc
     4021 taaatgtaaa taagtgaata atgtttgccc ttcacgtaac cgtttacatt caataggctg
     4081 gggctctttt actttgacta ttaattcggc attggcaaat acgtcatcag cagtatcaac
     4141 cacttcagcc ccggcatttc tataatcatc atccgatatg ccaataccaa gcccagcccc
     4201 cttctctacc aatacatcac ttcctgccct aataatttct ctaacactcg agggaacaag
     4261 tcctactctg ttttcgtgcg atttgatttc ttttggtacc cctactaaca tattgttacc
     4321 tcttatcgat tctttaattg ttctattaat tgtggaacta attcaaacag atcacctact
     4381 aacccataag tcgcaatttg aaaaataggt gcatctgcgt ctttattaat tgcaacaata
     4441 accttggaat ccttcatacc tgccaaatgt tgaactgctc cagaaatacc cacggcaaca
     4501 taaagcatag gagccactac cttcccggtt tgacctacct gataatcatt aggaacaaaa
     4561 ccggcatcaa ctgcagctct ggatgcccct actgctgcgc ccaaagtatc agctaattct
     4621 tcgattaatt taaatttttc tgcattttgt agccctctac cgccagaaac aactattttg
     4681 gcactaccta aatccggcct ctcggattta cttaattcat ggcgcacaaa ttgacttcct
     4741 tgggcttgaa attccttatc aatactttct atgcaacata cttcctggct tgatgtgatg
     4801 ggatcaaaag ctgtcgttct aatagtcatt acttttaatg aatccaaaac acgcacagtc
     4861 tctatggcat taccagcata aacaggatgt tcaaatgtgt ctgaattaat tatcttgctc
     4921 acatcagaaa tctgggtcac atcaagttgt gcagcaactc tgggcaatat atttttccca
     4981 taagtactgg aaggagctaa aatagcactg aatgaattcg caaaagacac caccaaatcg
     5041 ctaactgctt cagccaattg atgttcgtaa caaggattat ctacatgcaa aacagcatga
     5101 gctccttcca gactggctgc ctcctcggct actgaacggc attgatatcc aaccactagt
     5161 aatgtaattt tatcaccgag ttctaaagca gcagacagtg tattgcgggt tgctggatgt
     5221 aactgctgat tattatgttc aactaaaact aaagtgctca tctcatctcc ttaattaaag
     5281 cactttggct tcatgttgta atttatccaa caatgcagcc acagaatcaa gtatgacacc
     5341 accacttctc gttgccgggg cattaacctt taatatttca acgtgttgtt taagagataa
     5401 gccaagcgaa tccagatcaa tgatatcaag gggctttcgt ttggccttca taatattcgg
     5461 caagctggca tatctgggct catttaaacg aagatcggta ctgattactg caggcagctg
     5521 tacctttagt gtttccaaac caccatcaat ttctctcacc acttcaagat tattattatc
     5581 gggaatgatc ttcgacgcat tggtagcctg tggccaattc aataatgcag ccaacatttg
     5641 aggagtttga ttgttgtcac catctatcga ttgtttgccc atcaaaacca aatccggttt
     5701 ttcattagct acgatattat ttaatatctt ggctatatta aggctctcga atgatttttc
     5761 tgttcgaacc agaatggcct tatccgcgcc caaagctaaa gcgtgcctta aagtttcctg
     5821 agagctatcc gcaccaatgc ttacagcaat aacttccgtt gcccaatttt tttctcgcaa
     5881 acgcaatgcc tcttcaatgg ctatttcatc aaatgggttc attgccattt tgatattttg
     5941 agtttcaact cctgaattat ccgatttcac tcgaattttg acataagggt caatcacacg
     6001 ttttacagcc acgagtattt tcatggttcc tcactggaat atcaaatcag tctgcatctt
     6061 gccagagata ggggtataaa ttcaagaggt tgattttaat tagaacataa agctaattta
     6121 ttctaaatga gctatgttaa agaaaacctt tttctattct cacctgcctt ataatctggt
     6181 ccgggttgga aaactctact catgcaaaaa gaaaaaacat agggcaaaat aatgccctta
     6241 agcacaatta acagtgcaaa caatggttat attttgtcat tcacgcatag gtaaggaagc
     6301 tgtttttata gcaaactgac gatcgagatt tgcaatagct tcactcatac acaccaatca
     6361 cacacataat ccgttattta accaatgtgt gattttgccc tgagaataag catgagatac
     6421 ttgcaacagc tattaagtcc cattataaac caccaattgc atagaatcct tgtcttgtag
     6481 cagagagata acttgttctg cttcttttac ggcttttgct gtattgcttg taaaaacctt
     6541 agtatccttc tttatcttat ccagttcttt ttgagcttct accaatttct cctccaaagg
     6601 actaggaaga gaatccagta ctctcatctt gaataaaaat gcctctactt ttaaacgttt
     6661 ctctcgacct atcctcaagt caccaaaaaa ttgctgcgcg tttaaaccaa gataagattc
     6721 aagaggagca gaaatgactt ttaactccgg tgtcttgaat tgcatccctt caccagtatt
     6781 atggctagcc aagattaaat ctctacataa attggcagct gtatcagact ctgatttttc
     6841 cttagtctca ggagcgggta acataggttt taaaatcgca gagccgattt gtaaactcac
     6901 atcaagaaat tggcgcgaag cccaaaaagt catttcaagc aggcgaacta atagagtagt
     6961 tgatgcagtt gcagtaataa tactgatttc ttgcaacgtg cagcctattt tttttaattg
     7021 actggattca gtgctctcca tgtactgccc tcctttcaat aaaataaaat aaagcgcata
     7081 gatagcaaac aattcttctg caaaaagaaa agtgccatag ctggcaaata ctactggaat
     7141 aaatcgaagt aaaactctga gtcctctagg ttttattttg ccagtatcat caaaaaaacc
     7201 actggattgt tctaataaat taattaaact gtcacaaaag ctctggccag aataggtagt
     7261 tgccttggat cttaaatttt ccagccattg tttaaaatca tccttgcttt tgatatctcc
     7321 taaactacta tctttgaatt tttcaggtga aattaatttc tcatagcaca agccaagtat
     7381 cttgaagcat gctccattat tcttgctctg agataaatta ttctctagcc aaaccatttc
     7441 tggcgaaaat cctgaagcat gatcgggatg aaagcagaga gttagtcttt tatagttttt
     7501 aacaattttt tctttttctg gcccatctat caataattgc tggttaataa atcttgcaaa
     7561 atctctttct aatctcaata aggtatcctt atctaaatgg gatttataac aattattagt
     7621 tgtgtcccat tccgcataca atcttacaaa attttttaat tccctttcta tagtcataat
     7681 tactccctta ataaatttga ctacgattta tagagtctaa tttatcttat tgagatagtg
     7741 gttcctttgc aaatactgtg taaataagcc aacaagcaac atactgatat aaaatccagt
     7801 cataacatca cttaaataat gatgatataa aataaccctg gtagcagcga caagcaaggc
     7861 tgctccgata aagaggaaaa aatatcttgg gaagagaaac cccaatcccg cagccaaagc
     7921 agttatagta atagaatgcc ctgaagggaa tgaccaataa ttgtcattgt gttgaaacca
     7981 ataaaaccca tagaggttat tatcaaacaa gagatccggc cgcgcacgac taaagctaat
     8041 ttttaacaca aaagtcaata aattaggcag caaaacacag cccaataaat accaagatct
     8101 aatttcatat tgtttatttt gctgaatgta tcgaaagtat aatgcggtga ttaaaaacaa
     8161 tgcaacataa atcttccatt ttcccaaagc cgtcaagtat gttaatgcat gagcattgac
     8221 tcgaagatcc aggctatgga aataagtagc caacgttcta tccaaaaaat aataag
//
LOCUS       DASLOE010000131         8202 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.132, whole genome shotgun sequence.
ACCESSION   DASLOE010000131 DASLOE010000000
VERSION     DASLOE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.132"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..591)
                     /locus_tag="U6309_002255"
     CDS             complement(<1..591)
                     /locus_tag="U6309_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN5505690.1"
                     /translation="MKNLLFLVLVILTNSLMAKENLVFAVIGDYGANSAAEAKVASVL
                     KSKNPQFILTLGDNNYTHGCWKTIDKNVGKYYHEYIGNYQGKYGKGSDINRFFPTLGN
                     HDWLARKTCLYQGTLPYFSYFTLPGNQSYYNFVRGPIHFFALDSDSHEPDGSKEGSKQ
                     YQWLTEQVQLSKAPFKIVYFHHAPLSSGKHGSNTRMQ"
     gene            complement(725..1672)
                     /gene="mdcH"
                     /locus_tag="U6309_002256"
     CDS             complement(725..1672)
                     /gene="mdcH"
                     /locus_tag="U6309_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit epsilon"
                     /protein_id="HEN5505691.1"
                     /translation="MKILFLFAGQGYHAESLFDIFRTDKKTTNYLKTMSSAAEIDFLQ
                     NNLEMTNPYSIQSIIGAYQLTLFSRLLPLSTNHQVDLAGYSLGEISAFLGSINANPRD
                     SFKLFRYRTQLMTSIVDNNIFAEYDLLSVVGKFILEEIQPVIAKHNCYIAIINSTQHV
                     VIGGKISDLRHLITALSQYPISHAKFLGVYLPSHTDFYSDKSKQFQQFLDENYGFYSL
                     KYPVISPLELNKIYNAKQERALLAQELYTTLQWHKVCALIGEYQYNLIIDLGPGDSMT
                     TILNKANTELFNTPLITASHYNSLQGLCNAIISLSSSYQ"
     gene            complement(1669..2511)
                     /gene="mdcB"
                     /locus_tag="U6309_002257"
     CDS             complement(1669..2511)
                     /gene="mdcB"
                     /locus_tag="U6309_002257"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEN5505692.1"
                     /translation="MQTFQHSLPNPYKIARYFAKKAVQALYDEVALYPKPGLVSFIDS
                     GAHQDMNGALFFRSLFGLRHYFFQVGLHTALGSAPKKLVTFGLNAEKTMLKITGGVNT
                     HRGAIFALGILCTSICKLSAKYHAFSAGDLHYAIIDQWAQYLEKEHQNSDTHGTLVTK
                     RYAVPDAKQTAIQGYKAVFHTYNELSNIIHDQTFFGLLAYQRLLLAIDDINILYRTGP
                     EGLEFARYHIQQGICQNNREKSIQKAIELHQLFSKKNISPGGVADMLGLIYFLRHVFS
                     REAK"
     gene            complement(2483..3106)
                     /gene="mdcG"
                     /locus_tag="U6309_002258"
     CDS             complement(2483..3106)
                     /gene="mdcG"
                     /locus_tag="U6309_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase
                     holo-[acyl-carrier-protein] synthase"
                     /protein_id="HEN5505693.1"
                     /translation="MNYLRHTLCYLDKNTITASLPAPEQLDLLQYWLKCEYPLIVTRQ
                     PEDVPCGQIQLAIPYFIPTQSQKLRASYIVDDSWVIDTKALPSLQEIFPYLEFDTSTK
                     IQVYGSYCWQHLTREQYIQSSSDLDVLISYENQSLLTLSELQKHLQKTLKIDCIDGEI
                     RFPALGDCSWAELLQIHSSDSILFKAAKKIFLVKRESLYANFPALLA"
     gene            complement(3103..3822)
                     /gene="mdcE"
                     /locus_tag="U6309_002259"
     CDS             complement(3103..3822)
                     /gene="mdcE"
                     /locus_tag="U6309_002259"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HEN5505694.1"
                     /translation="MMPNEKITLKQLIDHCFDTTKTTIKQQIVFGAGKIENKNFNIVG
                     TVEDTSFGVDESIEMARCILDIIKNNNTDPIVLLTDVAGQKLSVRDEWLGMYAYFAHL
                     LKCLHLARQNGNKIITLIYNQAIGGSFIAFGMMADRIYALKNAKLAVMWLEGMSKVTK
                     IDIDVLRKISETSPVFAPGVENFKKLGGLHQVLDLDEVSQQLLLTLQEPNISHDNRAE
                     LGKQYGGRKEAYDIIKAIENL"
     gene            complement(3823..5019)
                     /gene="mdcD"
                     /locus_tag="U6309_002260"
     CDS             complement(3823..5019)
                     /gene="mdcD"
                     /locus_tag="U6309_002260"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEN5505695.1"
                     /translation="MDTYQYTFSLPKKLQISPMIKVGVAGSGNLEVIIKPNSDCDKTD
                     IIVNTVISGFRNTWDAVIVRFVEDYPYGGLSITLNDAGATPPVVSLRLRQAMETYQTG
                     YPKKDSYTETNARTRIYSLVDEASFTEFLLDKETPSPTLPQLNMQVETDDGVIIGIAK
                     MDGVDIAIASQQKDFIGGSVGEIHGAKINGLIKYAIKHQLPAIIFLIDSGGVRLQEAN
                     VGEIEISEIIRSILDARSAGIKTIGVICGNNGAFGGMGIISGTLDYLIVNQGARIGIS
                     GAEVIQAVKGVEVFDSSNRPLVWRVYGGRTRFLKDDVQGYTTNKTMDIRQAIKTALKT
                     LPTTPSLSLNSILAEHEQLQKRITSAKNCREESEWLKNNRPELYQQDIFNVSDQQFLA
                     LTNKGK"
     gene            complement(5028..6674)
                     /gene="mdcA"
                     /locus_tag="U6309_002261"
     CDS             complement(5028..6674)
                     /gene="mdcA"
                     /locus_tag="U6309_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEN5505696.1"
                     /translation="MDWNKNLKDYESRLINIKPFFKKGKIVSEKNIIALLETAIKPHD
                     KVCLEGDNQKQADFLARSLCSVNPQKIYGLHMLQSAVTLSEHLDLFERGIAEKIDFAF
                     AGPQSTRLAIMIQRNQVKIGNIHTYNELYARYVMDLTPNVALITAEKADYYGNLYTGA
                     NTEETPSIVEATAFKNGLVIVQANEIVKKLPRIDIPGEWVDAIVQAPSPSYIEPLFTR
                     DPALIDEVKILMAMMVIKGIYAPYQVNRLNHGVGFNTCAIELILPTYAQSLGLKGKIC
                     KHWMVNPLPTLIPAIEAGFIESIFCVGGEVGMNEFIRSKPDIFFTGRDGSLRSNRLYG
                     QLAGHYGCDVFIGATLQMDPQANSSTATKGRIAGFGGAPNMGCDTLGRRHSSYAWLKA
                     GQEKNSHNPVAMPRGRKLVIQMVETFQGAGKPTFVEKLDAWELQKEMQADLPPIMIYG
                     DDISHVVTEEGIANLLLCKNSEEREQAIRGIAGYTPVGLKRDKAIVDELRHRGIIQRP
                     EDLNISLKEADRDLLAAKNIHDLVEISNGLYCPPNKFRNW"
     gene            6954..7760
                     /locus_tag="U6309_002262"
     CDS             6954..7760
                     /locus_tag="U6309_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505697.1"
                     /translation="MDMKEKYTGRSEPIINKPSDIPKGLVAKRKAELLLAKLEEAIKS
                     QESLNRESESDAKEGNSESLKPRLTHLTSERPKINHRPPSSFSYTNSDSTLFTSVKEE
                     KTEGEVSSVSLSNDKKKVEDNSSASIWTELIAALSRAVDNYTKKKMEGTTPGEAYGWF
                     SWLRHGKAGEDKAGGLASEARTTSTVESLLTLLHHFFADSTTRYNNHSLAPYVMDELN
                     KLLVQYALKPFILSLGAQYDLNSWLHIKNQLSQLAGKEVCQERQSLVAPV"
BASE COUNT         2434 a         1526 c         1618 g         2624 t
ORIGIN      
        1 ttgcatacgg gtattggagc catgtttacc agaactcaac ggagcatgat gaaaatagac
       61 tattttaaaa ggagcttttg atagttgtac ttgttcagtt aaccattgat attgttttga
      121 accttccttg cttccatccg gttcatgaga gtcactgtcc aaggcaaaaa aatgaattgg
      181 accacgaaca aaattataat aactttggtt tccaggaagg gtaaaataac tgaaataagg
      241 taatgtaccc tgatataaac aggtctttcg agcaagccaa tcatgattac ccagagtagg
      301 gaaaaagcga ttgatatcag aacctttccc gtattttccc tgataattgc ctatgtattc
      361 gtgataatat ttacccacat tcttatcaat ggttttccag caaccgtgag tgtaattatt
      421 atctccaaga gtaaggataa actggggatt ttttgacttg agaacgctgg ctactttggc
      481 ttccgctgca gaattggcac catagtctcc aatgacagca aaaaccaggt tttccttggc
      541 catcagcgaa ttcgtcagga taactaaaac aagaaacaac aaatttttca aagaagatcc
      601 ctgtgcttga tggagcaaag ccgctatggt aagagatctc tttttaataa atcaatagaa
      661 ttgaaagtac aaaatgatta agtcaattta tttttaaacc ggtcgaaatt tagtgaatat
      721 tatttcattg ataagatgag ctaagcgata ttatggcgtt acaaagtcct tgcaaactat
      781 tgtaatgtga tgcagtaatt aggggggtat tgaacaattc tgtgtttgct ttgttcaaga
      841 tagtagtcat ggaatcgcca gggccgagat ctataatcaa attatattga tattcgccga
      901 ttaaagcgca aactttatgc cattgaagcg ttgtatatag ttcttgggca agcaaagccc
      961 tttcttgctt ggcattatat attttattta attctagcgg gcttatgaca gggtatttta
     1021 gagaataaaa accataattt tcatccagaa attgctggaa ttgtttgctt ttatcagagt
     1081 aaaaatccgt atgagaaggc agataaactc ctaaaaattt cgcatgactt atagggtatt
     1141 gagaaagagc tgtgatcaag tgcctgagat ctgaaatttt tccgccgatc accacatgtt
     1201 gtgttgaatt gataatagca atatagcagt tatgttttgc aataacaggt tgaatttctt
     1261 ccaatataaa ttttccaacg acagacaaca aatcgtattc tgcaaagata ttgttatcaa
     1321 ctatcgatgt catgagttgg gtacgatacc tgaataattt aaaggaatct cgaggatttg
     1381 cattaatgct ccctaaaaaa gcgctgattt ctcctaaact gtaaccagcc aggtcaactt
     1441 gatggtttgt ggagaggggc agcagtcttg aaaaaagggt taactgatag gcgccaataa
     1501 tagattgaat agaatatgga ttagtcattt ccaggttatt ttgtaaaaaa tcaatttcag
     1561 ctgcagaaga cattgtcttt agatagttgg ttgtcttttt atcagttcga aaaatatcaa
     1621 acaaactttc agcatgataa ccttgcccgg caaaaagaaa taatattttc atttggcttc
     1681 tcttgagaaa acatgccgta aaaaataaat taagcctaac atatctgcta cgcctccggg
     1741 acttatgttt tttttggaaa ataattgatg cagttcaatg gctttttgta tggatttttc
     1801 ccgattgttt tgacatatgc cttgttgaat atgataacgg gcaaattcta atccctcggg
     1861 acctgtgcga taaaggatat tgatgtcgtc aatagccaat aataaacgtt ggtatgctaa
     1921 taatccaaag aatgtttgat cgtgaataat gttcgacaat tcattataag tatggaatac
     1981 agccttatac ccttgaatgg cagtttgttt ggcatcaggt acagcatatc ttttcgttac
     2041 taatgtacca tgtgtatcac tgttttgatg ctccttttcc aggtattgag cccattgatc
     2101 aataatcgcg taatgcagat ctccggcaga aaaggcatga tattttgcac ttaatttgca
     2161 aatcgaagta cataatatac caagggcaaa tatagctcct cgatgagtat tgacccctcc
     2221 agtaatcttt agcatggttt tttcagcatt taaaccaaag gttaccagtt tttttggcgc
     2281 agatcctaag gcagtatgta ggccaacctg gaaaaaataa tgacgcaagc caaatagact
     2341 gcgaaaaaat aaagccccat tcatatcctg atgcgcccca ctatcaatga aactcactaa
     2401 tccaggtttt ggatataggg ccacttcatc atacagggct tgaactgcct tttttgcaaa
     2461 atatctggca attttgtatg gattaggcaa ggagtgctgg aaagtttgca tataggctct
     2521 ctcttttcac taaaaatatt ttttttgctg ctttaaataa aatggaatca ctggagtgga
     2581 tttgtaacaa ttctgcccaa gaacagtctc ctaaggctgg aaagcgtatt tcaccatcga
     2641 tacaatcaat tttaagcgtt ttttgtaaat gtttttgtaa ttctgacaag gtgaggagtg
     2701 attgattttc ataagatatc agcacatcca ggtctgaaga agattgaata tattgctccc
     2761 tggttaggtg ttgccagcag taggacccat aaacctggat tttggtacta gtatcaaatt
     2821 ctaaataagg gaatatctct tgcagagaag ggagtgcttt tgtgtcaatt acccaagagt
     2881 catccactat atagctggca cgtaattttt ggctttgcgt tgggataaag tagggaatgg
     2941 caagttggat ttgtccacag gggacatctt caggctgcct ggttactata agggggtatt
     3001 cacattttaa ccaatattgg agtaaatcaa gttgttcagg agcgggtaag cttgcagtta
     3061 tcgtgttttt atcaagatag catagagtat gtctgagata gttcatagat tttctatggc
     3121 ttttataatg tcataggctt ctttgcgccc gccatattgt ttgcctaatt cggcacgatt
     3181 atcatggcta atattgggtt cctggagtgt caatagcaat tgctgagaga cttcatccaa
     3241 atcaagaacc tggtgtaacc ctcctaattt cttgaaattt tctacgcctg gagcaaatac
     3301 cggggatgtc tcactgattt ttcgtaacac atctatatct atcttggtta ctttagacat
     3361 accctctagc cacatgactg ctaatttagc atttttcaaa gcatatattc tgtctgccat
     3421 catgccaaat gcgataaaac tgccgccaat ggcttgattg tatatgaggg taatgatttt
     3481 attgccattt tggcgtgcca aatgcaaaca ctttaataaa tgagcaaagt atgcatacat
     3541 tcctagccat tcatcacgta ctgataattt ttgtccggct acatcagtca ataggacaat
     3601 aggatctgtg ttgttgtttt tgattatatc cagaatacat cgggccattt ctatggattc
     3661 gtcaactccg aaactggtgt cttcaacggt gccaacaatg ttgaaatttt tgttttctat
     3721 tttcccggct ccaaaaacaa tttgctgttt aattgttgtt tttgttgtgt caaagcaatg
     3781 atcgattaat tgttttaaag taattttttc attgggcatc atttatttcc ctttattagt
     3841 caatgccagg aattgctggt cagaaacatt gaatatgtct tgctgatata attcgggtcg
     3901 gttatttttt aaccattcac tttcctcacg gcaatttttt gctgaggtaa tgcgtttttg
     3961 cagttgttca tgctctgcca gaatagaatt cagactaagg gacggggtag tggggagcgt
     4021 tttaagggca gtttttatcg cctgacgaat atccatggtt ttattggtgg tatatccttg
     4081 gacatcgtct ttaagaaagc gggttctacc gccatagaca cgccagacta atggacgatt
     4141 actgctatca aatacttcaa ctccttttac tgcctgaatt acttccgctc ctgatatgcc
     4201 aatacgtgct ccttgattaa cgataagata atcaagagtg ccactgatga tccccatgcc
     4261 accaaaggcc ccattattgc cacaaataac gcctatggtt ttaatgccgg ctgagcgagc
     4321 atccaggatg gagcgaatga tttcagaaat ttcaatctca cctacattcg cttcttgcaa
     4381 acggactcca ccactgtcaa tcaaaaaaat gattgcaggc agttgatgtt taattgcata
     4441 cttgattaga ccattgattt tggcaccatg tatctctcct actgacccgc caatgaaatc
     4501 tttttgttgc gatgcaatag ctatatcaac tccatccatt ttggcgatgc caatgatgac
     4561 cccatcatca gtttccactt gcatgtttaa ctgaggcaag gttggactgg gtgtctcttt
     4621 atcaagtaaa aactccgtaa aactggcttc atcgactaat gaataaattc tagtgcgagc
     4681 gttcgtttcc gtatagctat cctttttagg atatcctgtt tggtaggttt ccattgcttg
     4741 acgcaggcgt aaggaaacga caggaggagt ggcacccgcg tcgttaagag taatggatag
     4801 cccaccataa ggataatctt caacaaagcg tacgattaca gcatcccagg tatttcggaa
     4861 accagagatt acagtattta caataatatc ggttttatca cagtcagagt ttggtttaat
     4921 aataacttcc aaattacctg aaccggccac tccaacttta atcattggag atatttgaag
     4981 ttttttgggt aaactgaatg tgtattgata cgtatccata aataggtcta ccaattcctg
     5041 aatttgttag gaggacaata aagtccattc gaaatttcca ctaaatcatg aatgtttttg
     5101 gcagcgagta gatctcgatc agcttctttt aatgaaatgt tgagatcttc tggtcgttga
     5161 atgataccac gatggcgcag ttcatcgact attgctttat cacgtttcaa acctaccgga
     5221 gtataaccgg cgattccgcg gatagcttgt tcacgctcct ctgaattttt acataataac
     5281 agattggcta tcccttcttc ggtgacgaca tgagagatgt catcaccata aatcataatg
     5341 ggcggcaggt cggcctgcat ttctttttgt agttcccatg catccagttt ttctacaaag
     5401 gttggtttgc ctgccccctg aaatgtttca accatctgta tcacaagttt acgtcctcga
     5461 ggcattgcta caggattatg agaatttttt tcttgtccgg ctttaagcca tgcgtaagag
     5521 gaatgacgtc gccctaaagt atcacagccc atattgggtg ctccaccaaa gccggcgatt
     5581 cttcctttgg ttgccgttga actgttggct tgcggatcca tttgaagagt cgcgccaata
     5641 aatacatcac atccataatg cccggcaagt tgcccataca agcgatttga acgtaatgaa
     5701 ccatctcgac cagtaaaaaa gatgtctggt ttggagcgaa taaactcatt catccctacc
     5761 tcacccccta cacaaaagat agactcgatg aatcccgctt caattgcagg gattaaagta
     5821 ggtagaggat tgaccatcca atgtttgcaa attttacctt ttaaacccag agactgggca
     5881 taagttggca gaataagttc aattgcacaa gtgttaaaac caaccccatg atttaatcga
     5941 ttgacttgat aaggagcata aatgccttta atcaccatca tggccatgag aatttttact
     6001 tcgtcaatga gagctggatc tcttgtaaac aaaggctcaa tgtaggaagg ggatggggct
     6061 tgaacgatgg catcgaccca ctctcctgga atgtcaattc ttgggagttt cttgactatt
     6121 tcattagctt gtacaatgac caggccattt ttaaaagcgg ttgcttcaac gatactcggt
     6181 gtttcttccg tattggcgcc ggtgtataga ttaccgtaat aatctgcttt ttctgcagtg
     6241 attaaggcga cattgggagt aagatccatc acataacgtg cataaagctc attgtaagta
     6301 tgtatattgc ctatcttaac ctgattacgc tggatcataa ttgccaaacg agttgattgt
     6361 gggccagcaa acgcaaaatc aattttttca gcaattcctc gttcgaacaa gtcgagatgt
     6421 tccgataagg taacagccga ttgcaacata tgtaatccgt atattttttg gggatttaca
     6481 gaacataggc tgcgtgccag aaaatcggcc tgtttctgat tgtctccttc caggcagact
     6541 ttgtcatgag gttttattgc tgtttcaaga agggcgatta tatttttctc agaaacgatt
     6601 ttaccttttt taaaaaaagg tttgatgtta atgagtctgc tctcataatc cttgaggttt
     6661 ttattccagt ccataatttt ttagtaatcg gatgagaact atagtgatat caaatgttaa
     6721 tagtatagta gatattgacg caagtatttg attttatcct ctgctttttt cttgtggctt
     6781 gtgcaaggta agttggccta tgatatttct ctgtaactga aaaatacaaa atattttatt
     6841 tcagtgtatt tctgttttct ggttgaacag aagcaattaa ttccttgaaa gaaaataata
     6901 taatgcatta acattaatga actgtttaaa ttcataaatc atgacatagg gaagtggata
     6961 tgaaagaaaa atataccggt cgctcagagc ctattatcaa taaaccttct gatattccta
     7021 aaggattggt tgcaaaaaga aaagcagaat tgctcttggc aaaattagaa gaagctataa
     7081 aatcgcaaga atctttaaat agagaatcag agagtgatgc gaaagagggt aattctgaat
     7141 cactcaaacc ccgattgacc catctaacga gcgaacgtcc aaaaattaac catagacctc
     7201 cttcttcctt tagctatacc aattcagaca gtactttgtt tacttctgtt aaagaagaaa
     7261 aaaccgaggg ggaggtgtcg tctgtatccc tttctaatga taaaaaaaaa gttgaagata
     7321 actcttctgc atcaatttgg acggaactga ttgctgcctt atcaagagca gttgacaact
     7381 atacgaagaa aaaaatggaa ggaactactc ccggtgaagc ttatggttgg ttcagctggt
     7441 tgcgtcatgg caaggcaggt gaagataaag caggagggct cgcaagtgaa gcacggacaa
     7501 ccagtacggt tgaatctttg ttgaccttac ttcatcattt ctttgcggat tctaccacca
     7561 gatacaataa tcattcattg gctccttatg tgatggacga gctcaataaa ttattggttc
     7621 agtatgctct taaacccttc atacttagtt taggagccca atatgatctt aatagctggc
     7681 tgcatatcaa aaatcaatta agtcaactgg caggtaagga agtgtgtcaa gaaaggcaat
     7741 ctcttgttgc acctgtgtga ttatcaggag tcctgcacag gactccgtat tttttgatcg
     7801 cttgagttta aaagtttaaa tttttttgaa ttgtttgatg attgcttgat tccaatcttt
     7861 gcgatgcaga aattgcagca aatgccaaat taaatgtggt tgtgtatgta ctgccagaac
     7921 actgccttaa tagccttaat aaacaaattg tgaagacacc ccctgtctat ttcagttggc
     7981 gatacagata tatcaaaaaa atctctttga gccagtctct tttatttttg tacataattt
     8041 gatcatttaa aatatcacca gctattttaa caatttttgt gcttcgttat gagggcatgg
     8101 aatttttatt aagcagagca catgttgtat ttaaattata aacattgcgc tttttatata
     8161 ttgtatttta tgttactgtt caatttcttg atcaataaaa tc
//
LOCUS       DASLOE010000132         8184 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.133, whole genome shotgun sequence.
ACCESSION   DASLOE010000132 DASLOE010000000
VERSION     DASLOE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.133"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            522..618
                     /gene="ffs"
                     /locus_tag="U6309_002263"
     ncRNA           522..618
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U6309_002263"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            644..2314
                     /gene="dnaX"
                     /locus_tag="U6309_002264"
     CDS             644..2314
                     /gene="dnaX"
                     /locus_tag="U6309_002264"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEN5505698.1"
                     /translation="MSYLALARKWRPRTFSQLVGQEHINKALINALNQQRLHHAYLFT
                     GTRGVGKTSVARILAKALNCEKGITSEPCLHCDTCVAIEQGRYIDLIEIDGASKTRVE
                     DTRDLLDNIQYAPTNGRFKIYLIDEVHMLSQHSFNALLKTLEEPPSHVKFILATTDPQ
                     KLPVTVLSRCLQFNLKHLPAELINQQLQLILQEECLDFEIQALDILAQAARGSMRDAL
                     SLLDQAITSCETKLLASDVKMLLGHTQQDYALLLLQALAKQEAPTMLVLSQQIIAEGG
                     NFQYVLDEMLNYLHQITIYQCLGDNNPLISPKAEIKELAKQFSAEDTQLFYQIGIKGR
                     EEMHLAPTLAIGFNMTMLRMYTFKPASHVTTPPLAFEQNNKTCSLIIDDNTPAQNPVG
                     IEPIPVTKATGNTVAYHQTQSLSTDDKKSANSGESMNCDWNDIIPQLKLSGLALNAIE
                     NAEFLGKKEREITLGVQKGHQSLFTPTTINRIETALAGYYKSPVKIILNSQDAVHASP
                     AQQKEKATQEKQEQAEAALHADPVFQQLKQEFSAELVKNSIVLLKDDL"
     gene            2348..2686
                     /locus_tag="U6309_002265"
     CDS             2348..2686
                     /locus_tag="U6309_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEN5505699.1"
                     /translation="MDINQNLGNLMKEAQKMQQRMQEAQQQLSQLVVSGESGGGMVTI
                     KMNGRHDVTEVKIKPTLMDEDIEMLEDLIAAAVNDAVRKIEKASKEKISQLTAGLNIP
                     TDLMGGKEGE"
     gene            2695..3288
                     /gene="recR"
                     /locus_tag="U6309_002266"
     CDS             2695..3288
                     /gene="recR"
                     /locus_tag="U6309_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEN5505700.1"
                     /translation="MDALSRLVEALRCLPGVGPKSAQRMVFHLLQHQRQRGLHLASCL
                     EQAMKHISHCQQCNNYTEQTLCTLCQNPNRDSTLLCVVESPADVSAIEQSNSFQGKYF
                     VLMGKISPLDGLGPDDIGLPKLKELIIREKIQEVILALSPSVESQTTIHFIHQLLKDE
                     TVNISQLAHGIPSGGELEFLDGNTISSALKNRAVINV"
     gene            3281..4141
                     /locus_tag="U6309_002267"
     CDS             3281..4141
                     /locus_tag="U6309_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEN5505701.1"
                     /translation="MSKSIFILCRKKILVLLLLSLCFITSTASASFHRSLWPKWLVNA
                     PLSKQSISHQAWQHFLDHHVITNEEDINLVDYTNINEKELASLKEYIKNLSQIDIDNY
                     NRQEQLAYWINLYNALTVLTVANYYPIANIQEINISPGLFSVGPWGANIITIKNTNLS
                     LDDINNRIIRPVWNDPRTHYALNNATIGAPNLSKQAYQGSLLEQQLNDAAFKYINSLR
                     GVHVIEGKLIVSKLYDWYEEDFGGTKKYVIKHLLQFAKEPLRNQLKHINTIDSYIYNW
                     HINCPADNQS"
     gene            4138..6135
                     /locus_tag="U6309_002268"
     CDS             4138..6135
                     /locus_tag="U6309_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEN5505702.1"
                     /translation="MKKKHSIFAYFSQFLLFNLCFFGLQLLLLYSHAESLISSIKLPL
                     PIYFEIIGTLVIQFSLYIIISMIQALLLIGILNRPWHYFSTEQWQTIIWSLFACAILS
                     ANAYYFPLSVFSKLFSPPIPEFIFLILLYFSLACLGLLLLNCLFYRMTARLLSIALPI
                     GICIFLLNNYWKSSLNPNVDSEPNVIILGIDSLSPESVTSRNMPFLHQVLLDSFQFTN
                     TISPLARTYPAWSSILTGLYPKHHLAEENLVERSRVKSQLSIVWDLNKLGYNTIYATD
                     DRRFNSIDENFGFKKIIGPKTGVNDVMLGSYNDFPLGNLLINFRISAWLFPYNFSNRA
                     SFISYYPETFTNELKRELSLQQNSPVFLAVHFALPHWPYAWAASLPDQVENEFSLSKR
                     DALYLRALKAVDNQFKVIFCYLKKHGYFKNSLVIILSDHGEVLYYPNSRLTSYQKYQS
                     SLTSKLAEYFKAKTATELDKSAGHGSDILSPMQYHSLLAFNVYKDGKPLTKTNKIKTR
                     VALFDLAPTILDFLNITKKQKMDGISLLPTVLNPNTPLPHRTFFIESGMYPNQDFTKE
                     KAIELGKKIYKVNPDTGQLELRPDELIQVNNQKLYGVISGNWILALYPDDNTYIPVIL
                     NLSTGEWIDNLQSDFAKSSPAEKLKRQLQEFYGKKLAYPIQ"
     gene            complement(6392..7861)
                     /locus_tag="U6309_002269"
     CDS             complement(6392..7861)
                     /locus_tag="U6309_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505703.1"
                     /translation="MTQKTLTLPNWFVTDNDRAKARIKYELKQLETQLGKCVKQPDDT
                     AFSKNKYSLYMALADKYSEYLKLIDIDSKFKQFVGAEIPKDISYREACTRIMHLKQKR
                     SAAVNLPEEAKIIIEISLLYQCLAKAIEPNEVSDVSNHLILNQFDKLKQLFKKENLEL
                     WLSTLVDLWNHNSILLREPAKLFRNWDLEEQTIALGFFSESEVVNLVNAIFFYKLYPD
                     KLFNELIHPEKLVSVRMRLGFLHEFIELLQKQLHSNALQYGLNPGIDFLFHGEELPQG
                     IMIEVNEVYRDIIQLAVKNLRVQFTPENNEKVTLERLHDLGRAYKFWFNPNRLIDAVM
                     VLQQRLVTESISQEEDLEKFHQEMLVLYRQLTTTECLDLYGYFANNDSRYLLYTLYSI
                     INGYSLDWLPSLNSSERNAIARVYQALKCVMEALRGELKNRFITTEPYLYDLGKKKIH
                     TGRRNREAVFRIIAIYGCERIVMSDSLERLFNLIEEPNQ"
BASE COUNT         2661 a         1621 c         1401 g         2501 t
ORIGIN      
        1 tttatctctc taagcactca aagtatctca aagtgcccac acagtttgtc ttctctcttc
       61 ttaatgaacc cgccctcgtg gcgtagggcg tattctactc atttcatcac cactgtcaaa
      121 tactttttta atttttttat cataaagcag aaagtctcac tttgtttgtg cagaaatgaa
      181 atggaaaaat gcttttgttt cacttaattg ctactgatgg atattgaaga tggtatttga
      241 taaatcctat ttacttgatt acttattact tattacttat tacttattac ttatataagt
      301 attgtttata acttaaattt ctatttcctg atactttgta gcttaaaagg aaatatttat
      361 tcatgtggat tggatatgct ctttattagc cctggttatt taattacttt tttaatttct
      421 atcctttagg aaagcagtta cactcaaata ataaaatagt cattagctct agcgcagaaa
      481 tatttttttt gttactcttc gctattcaaa tatggtgact ctattggtcc cctcgcgacg
      541 atagattgtg aaccccgtca ggcccggaag ggagcagcgg tagcaattga tgcgggcgcc
      601 ggggtgtggc tcttagagtc gccgcccaac ttgtgaaaca cgtatgagct atctggcact
      661 tgcccgtaaa tggcgaccac gcacattttc ccaactggtt ggccaagagc atattaataa
      721 agcactgatc aacgcgctga atcaacaacg tttgcatcat gcttatctat ttactggtac
      781 tcgcggggtc gggaaaacca gtgtcgcccg tattttagct aaagccctca attgtgaaaa
      841 aggaatcacc tcagaaccat gccttcattg cgatacctgt gttgccattg agcaaggccg
      901 ttatattgac ctcattgaaa tagatggagc ctccaaaacc agagttgaag acacgcgaga
      961 tttacttgat aacatacagt acgcaccaac aaatggccgt tttaaaattt atttaatcga
     1021 tgaagtgcat atgctatccc aacatagctt taatgcttta ctaaaaacat tagaggaacc
     1081 gccttcgcat gtgaaattta ttctggcaac gactgaccca caaaaattac ctgttactgt
     1141 cttatcgcgt tgtttgcaat ttaatctaaa acacttgcct gctgagttga taaatcaaca
     1201 attgcaatta attctccaag aggagtgctt ggattttgaa atacaagcac tagacatatt
     1261 ggctcaggct gcccgcggaa gcatgcgcga tgcattaagt ttattggatc aggctattac
     1321 cagttgtgaa acgaaattgc tggcaagtga tgtaaaaatg cttttgggac atacccaaca
     1381 agattacgca ttgctgctct tgcaagctct ggccaagcaa gaagcgccaa ccatgcttgt
     1441 attaagccag caaatcattg ccgaaggagg gaattttcaa tacgttcttg atgaaatgct
     1501 taactatctg catcaaatta ctatatatca atgcctgggt gacaacaatc cattaattag
     1561 tccaaaagca gaaattaaag aattagcaaa acagttttcg gccgaggaca cacaattatt
     1621 ctatcaaata ggtattaaag gacgtgagga gatgcatctc gcccctacac tggccattgg
     1681 ctttaatatg accatgctgc gcatgtacac ctttaaacca gcttctcatg taacaacacc
     1741 tccattagca ttcgagcaaa ataataaaac atgctcacta attattgatg ataatacacc
     1801 agcgcaaaac cctgttggaa ttgaaccaat acccgttact aaagcgacgg gcaacaccgt
     1861 tgcctatcat cagacccaat cattatcaac agacgataaa aaatctgcca actctggtga
     1921 gtccatgaac tgtgactgga acgacataat accacagtta aaattatcgg gtttagcact
     1981 taatgccata gaaaatgcag aatttcttgg taaaaaagag cgagaaatta cattgggagt
     2041 acagaaaggg caccagtcct tattcactcc cacaacgatt aaccgaattg aaaccgcatt
     2101 ggctggttat tataaaagtc ccgtaaaaat tattttaaac agtcaagatg ccgtgcatgc
     2161 ctctccagct caacagaaag agaaagcgac ccaagaaaag caagaacaag cagaggcagc
     2221 attacacgct gacccagtat ttcaacagtt aaaacaagaa ttttcagcag aattggtcaa
     2281 aaattctatt gtcctgttaa aagatgactt ataatactta aactaaccat aaatagacga
     2341 ggctgtaatg gatattaatc aaaatttagg caatttaatg aaagaagcgc aaaaaatgca
     2401 acaacgtatg caagaagcgc agcaacaatt aagccagttg gtagtaagtg gagaatcagg
     2461 tggtggtatg gttactatta agatgaatgg acgtcatgat gtaactgaag ttaaaatcaa
     2521 acccacctta atggatgaag atattgaaat gctggaagat ttaattgctg ctgcagtcaa
     2581 tgacgcagtg cgcaaaatcg aaaaagcatc caaagaaaaa atcagtcaac tgactgctgg
     2641 tcttaatatt ccaactgatt taatgggtgg taaagaagga gagtaaccct ggctatggat
     2701 gctctaagcc gcttggtaga agcgcttcgc tgtcttcccg gtgtgggacc aaaatcagca
     2761 caaaggatgg tgttccattt attgcagcac caaagacagc gaggtttaca ccttgcctcc
     2821 tgtttggaac aggcaatgaa gcacattagt cattgtcagc agtgtaataa ttacaccgaa
     2881 caaactctgt gtactctttg ccaaaacccc aatcgagatt caacattact ttgcgtggta
     2941 gagagtccag cagatgtttc tgccatagaa caaagcaatt cctttcaagg taaatatttt
     3001 gtccttatgg gaaaaatttc tcctcttgat ggcttgggac ctgatgacat agggctaccg
     3061 aaacttaagg agttaattat tagggaaaaa attcaggaag ttattcttgc attaagcccc
     3121 agtgttgaaa gccagaccac aattcatttt attcatcagt tgctaaagga tgaaaccgtc
     3181 aatatcagtc agttagcaca tggaatacca tcaggtggtg aattagaatt tttagatggg
     3241 aatactataa gcagtgccct taagaacaga gcagtgatta atgtctaaat ccatatttat
     3301 tttatgcaga aaaaaaatac tggtcttact cctgttatcc ttatgcttca taacaagtac
     3361 agcttctgct tccttccaca gaagtttatg gcccaaatgg ctggtcaatg cccctctttc
     3421 caaacagtca atttctcatc aggcatggca acattttctt gatcatcatg taataactaa
     3481 tgaagaagac atcaatctgg tagactatac caatatcaat gagaaagagc tggcttcatt
     3541 aaaagaatat ataaaaaatt tatcgcaaat tgatattgat aattataacc gtcaagaaca
     3601 attggcttat tggatcaatt tgtataatgc ccttacagta ttaacagtag ccaactatta
     3661 ccccattgca aacattcagg aaattaatat ttctcccgga ttatttagcg tcggaccttg
     3721 gggagccaat attatcacta tcaaaaatac taacttatcc ctggatgata tcaataatag
     3781 aattattcgc ccagtttgga atgacccaag aactcattat gctttaaata atgccaccat
     3841 aggagcgcct aatctgagca aacaagcata ccaaggatca ttgctggagc aacaacttaa
     3901 cgacgcggca ttcaaataca ttaactctct caggggagtt catgtgattg aaggtaaatt
     3961 aattgtttct aaattatatg actggtatga agaagatttt ggtggtacca aaaaatatgt
     4021 catcaaacat ttgctacaat tcgcgaaaga acctctgcgt aaccaattaa agcatattaa
     4081 tactattgat agttatattt ataactggca tattaattgt cctgcagaca atcaatcatg
     4141 aaaaaaaagc attcgatttt tgcatatttt agccaattcc ttttatttaa tctttgcttt
     4201 tttggtttgc aattgcttct gctctattcg catgctgaga gcctaatcag ttcgatcaag
     4261 cttcctttgc caatctattt tgaaattatc ggcactctcg taattcaatt cagcctgtat
     4321 atcatcattt ccatgattca agccctgctg cttataggca ttttaaatcg accttggcat
     4381 tatttttcta ctgagcaatg gcaaaccatt atttggtcct tatttgcatg cgctatatta
     4441 agtgctaatg cctattattt ccctctgagt gtttttagca aactattttc accaccaatt
     4501 cctgaattta tttttttaat attactttat ttttctttgg cttgcctggg gttactctta
     4561 ttaaattgct tgttttatcg catgactgct cgcctattat caatagcgct acccattggc
     4621 atttgtattt tcctattaaa taattattgg aaatcttcct taaatcctaa tgttgattcc
     4681 gaacccaacg tcattattct gggcatagac agcttaagtc cagaaagtgt cacatctaga
     4741 aacatgcctt ttcttcatca agtgctactg gacagttttc aatttacaaa cacgattagt
     4801 cctttagcca gaacctatcc cgcttggagc tccattttaa ctgggttata ccctaaacat
     4861 catcttgccg aagaaaattt agttgagcga agccgggtta aaagtcaact cagcattgtg
     4921 tgggatttga ataaattggg gtataacaca atctatgcga ctgatgatag acgattcaat
     4981 agcatagatg agaattttgg atttaaaaaa atcatcggcc caaaaacagg tgttaatgat
     5041 gttatgttag gctcttataa tgatttccct cttgggaatt tactgattaa ttttcgcatt
     5101 agtgcctggt tattccccta taattttagt aatagagcca gcttcatatc ctactatccg
     5161 gaaaccttta caaatgaatt aaaaagagaa ctgtctttac aacaaaattc ccctgttttc
     5221 cttgcggtgc atttcgctct tccacattgg ccttatgcct gggcagcatc attgcctgat
     5281 caagtggaga atgaattcag cctcagcaag cgtgatgcgc tttacctaag agcgctaaaa
     5341 gcagtagaca atcaatttaa agtcattttc tgttatttaa aaaaacatgg ctattttaaa
     5401 aatagcctgg tcattatact aagcgaccat ggcgaagtac tttattatcc caactcacga
     5461 ttgacaagtt accaaaaata tcaaagctca ctaactagca aactcgcaga gtattttaag
     5521 gccaaaactg caactgaact ggataaaagt gctggtcatg gttctgatat attaagccct
     5581 atgcaatatc atagccttct ggcatttaat gtttataaag atggaaagcc tcttacaaaa
     5641 acaaacaaaa tcaaaacaag agtagcatta tttgatttgg ccccaaccat tctggacttc
     5701 ctgaatatta caaaaaaaca gaaaatggat ggcatttcat tactacctac tgtattaaac
     5761 ccaaatactc cactaccaca ccgcacattc tttattgaaa gtggaatgta tccaaatcaa
     5821 gattttacta aagaaaaagc cattgaatta ggaaaaaaaa tctataaggt taatccggat
     5881 actgggcagc ttgagttgag gcctgatgaa ttaatccagg tgaataatca aaagctatac
     5941 ggggtcatta gtggaaactg gattttagcc ctatatccag atgacaacac ctacataccc
     6001 gttattctca atctttcaac tggagaatgg attgataacc tgcagagtga ttttgccaaa
     6061 tctagtccgg cagaaaaact aaagagacaa cttcaagaat tctatggtaa aaaactggct
     6121 tatcccattc aataaaagcg ctttataaag ctcactgcaa aacccccata caaaacagca
     6181 ggcctcttgc agaacttgat tattttgttt cagagcaagg cgcaaacgtt ttgagaagca
     6241 gagtttatat gtagtaaatg agcgccgcag aaacgtttac aatgcagcag taaaatgaaa
     6301 gaagcaagtt ataaaagagg ttgggtatat cccaaaaaac gaattactct tagtcaattg
     6361 gcacctgaaa aattcatatt tactactcaa attactgatt tggttcttca atgagattaa
     6421 acaatctttc taatgagtca ctcatcacta ttctttcaca accataaatt gcaatgattc
     6481 taaatacagc ctctcgatta cgtcttcctg tatgaatttt ctttttcccc aaatcatata
     6541 gataaggttc ggttgtgata aatctgtttt ttaattcgcc tcttagcgcc tccataacac
     6601 acttcaacgc ttgatacact ctggctatag catttctttc agatgaattt aatgatggca
     6661 accaatctaa agagtaacca ttgattattg aatataaagt ataaagtaaa tatcgactgt
     6721 cattgttggc aaaataaccg tataaatcca ggcattctgt tgtggttagc tgacgatata
     6781 gaactaacat ttcttgatga aatttttcta aatcctcctc ttgagatata ctttccgtta
     6841 ctaggcgttg ctgcagaacc atcacagcgt caattagcct gttaggatta aaccaaaatt
     6901 tgtatgccct tcccaaatca tgtaaacgtt ccagggtaac tttctcatta ttttcaggtg
     6961 taaattgcac tctaaggttt ttaacagcta attggattat atctctataa acctcattca
     7021 cttcgatcat aatgccttgg ggtagttctt caccatgaaa taggaaatca atacctggat
     7081 ttaggccata ttgaagcgca ttgctatgta attgtttttg tagaagctct ataaattcat
     7141 gcaaaaaacc aaggcgcata cgtactgaaa ccaatttttc aggatgaatt aattcattaa
     7201 ataacttatc aggatacaat ttataaaaaa atatagcatt tactaaatta acaacttcag
     7261 attcagaaaa aaaacctaag gctatcgttt gttcttcaag atcccaattg cgaaataact
     7321 tggctggctc ccttaataaa atagaattgt gattccaaag atcaaccaga gttgataacc
     7381 ataattcaag attctctttc ttaaataact gctttagttt atcaaactga ttcaggatta
     7441 aatgattgct tacgtctgaa acctcatttg gctcaattgc tttagcaaga cattgataca
     7501 ataatgatat ctcaatgata attttagctt cctccggcaa attcactgct gcagaccttt
     7561 tctgtttcag atgcataatc cgagtgcaag cttcccgata actaatatcc ttaggtatct
     7621 ccgcccccac gaattgttta aatttagaat caatatctat taacttcaaa tactccgaat
     7681 atttatccgc caaagccatg taaagtgagt acttgttctt tgaaaaggca gtatcatccg
     7741 gctgcttaac gcatttgcca agctgagtct ccaactgttt caactcatat ttgattcgcg
     7801 ccttggctct gtcattatcc gtaacgaacc aatttggtaa agtaagtgtc ttttgagtca
     7861 tccctttctc ctcaagcatt atcctgacac ttgaacttta acaatagcat tgaaaagaaa
     7921 tattttccta atttttataa cgaacaaaat gcaaattttg caataaacca ggttttacgt
     7981 tctaaaaagc tctggcaagc ataaatattt aaaaataatt gcaagcaact gataaacttg
     8041 ccaaaattaa agcaatatat tttttgacct ttgaggcaaa ttgatgtcac aacgccacta
     8101 tttgctattt attgataatg agcctgtcag tgaagaactg aaagattact ttgaaaagtt
     8161 taatcttgat attattcaac aaaa
//
LOCUS       DASLOE010000133         8055 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.134, whole genome shotgun sequence.
ACCESSION   DASLOE010000133 DASLOE010000000
VERSION     DASLOE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.134"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(122..1075)
                     /gene="ceg5"
                     /locus_tag="U6309_002270"
     CDS             complement(122..1075)
                     /gene="ceg5"
                     /locus_tag="U6309_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg5"
                     /protein_id="HEN5505704.1"
                     /translation="MISQKEALALLNKYEKHLNELQNIITTSYQNLLRCDAQIIPNAL
                     AALKAIANNTQFAPVVATSGMSSIKALNSDPQIALKELLVNDFFKDHFQKMCIHYSKD
                     NPELLTNLQNFHACISQEKIIIYKAKNGELALKFPTQEWRDRFIQKMGGVSHFASSDK
                     FNANKKCPQTYPENPTTLYLSTYTAKNGELAVVFPNKEIRQNFLTLLRHASSATWSIY
                     EGQDAVYFNDKNLHTPGYNFGINAPACVSQSSNAAHADVRYAARILGQALRNPNNTCF
                     FNRLPGELINKIISLSTDNEVLAAEDAISSVQPNLGKPQLK"
     gene            1453..2319
                     /locus_tag="U6309_002271"
     CDS             1453..2319
                     /locus_tag="U6309_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0189 family type II secretion system
                     effector"
                     /protein_id="HEN5505705.1"
                     /translation="MKPGYLLGALLLSPLLTFANSDNSSDGLIFSPLPQNKNTVVRHY
                     SNEQDMSNLNQMTQRTIDFPTQIVRVSGNLTGLELSCDDVENEIDQIFSKKISPNLFT
                     YNTYVSCGFDEHDPEQYATNFSIQSYFDPLTDNAVDYLKTYLKEYNGYNLFNTTTLQI
                     ENAKGIIVSMNLNAGLKNNPDKTPFMLYRQDRNNFYFKSNFDMRKELITDIYQRFYSN
                     DPDMILPFFDKWIFSYAGSVYYSILKASNYLELQPERIFVMENEGDIFVSDLRYYFAN
                     LCMKRNPNKHCL"
     gene            complement(2515..3567)
                     /locus_tag="U6309_002272"
     CDS             complement(2515..3567)
                     /locus_tag="U6309_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEN5505706.1"
                     /translation="MLKGLKVIDLSTVLAGPSVGTFFAELGASVVKIEHPVHKDVTRT
                     WKLPGEDPHSEISAYFASVNFGKDYLFLDLMKEEDYLCFLNLIAEADILIMNFKKGDD
                     IKLRIQADHLLSINPRLIIGKINGYGEESERVAYDLVLQGESGFMSINGTPDSGPVKM
                     PVALIDVLAAHHLKEAILLALYNREKSGKGKVVSVSLYDAAVSSLVNQASNYLMTGQV
                     PQRIGSLHPNIAPYGELFRTADAALIILAIGSDRHFAKLCAVLGLNDLPDNDEFKHNQ
                     NRVRNREKLALLIAEQTAKWQSNKLLQMLHRENVPAGKIMDIKDVFDDERAKSLIRKE
                     FIGDIPTARVASVVFK"
     gene            complement(3706..5115)
                     /locus_tag="U6309_002273"
     CDS             complement(3706..5115)
                     /locus_tag="U6309_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEN5505707.1"
                     /translation="MIRKKITQISFFMILALIVGAVEAKTTRPEDEQQLARSIYKQFI
                     EVQSGFTTGSTTPLVEVAVKYLKTAGFSNEDIFVGGASPQKANLVVRYRGTGEKKPLL
                     LLAHTDVVEAKASDWSMNPFQLTEKDGYFYGRGTLDDKAQAAIWIANLIQYKQEGFKP
                     KRDIIVALTADEEGSSPYNGISWLIKNHKDLIEADFALNEGGWGDLANGKKISQNIQV
                     SEKYIVNYNLEVRNKGGHSSLPTKDNAIYRLAGALERLSKFNFPLKTNDVTAAYFKQM
                     AAIETGPLKTEMIEASKGSKQAMQRLADSSPQWNAILRTTCTPTLLEGGHAMNALPQL
                     AAVTINCRVLPEDSPEMVEQSLKTAINDPEVTLKRIGKLSRGPSSPLSPEILKTITHL
                     TQTYWPEVPAIPIMVTGATDGRYLRSAGIPTYGVMGLFLDRDDFRAHGRDERISVESF
                     YEAHAFLYDLVKQLSSNVT"
     gene            5295..6236
                     /locus_tag="U6309_002274"
     CDS             5295..6236
                     /locus_tag="U6309_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN5505708.1"
                     /translation="MYKIHHYFLFGVLVPFIGLVSLFTIFSSDFLTLTTWASILASAS
                     DIGIVVLGVTLLLIGGEFDLSVGANFALSGLLFGSLSLLGINSILALIIALTIGACIG
                     LVNAACTVFFKIPSFIVTLGSMLILRSLILLYTQGLPISIEYTNVVMQVLAGELSQNL
                     LSSFIWWIVIGFYLIYLLNWTKLGNHIIATGNNCDSAYLSGINTMRVKFFCFVCCGII
                     AAFAGVTQFSHLNSLSPIAGEQYELYAIAATVIGGTSLKGGQGSIISTLLATLLLSTV
                     DTGIVQIGVSTYWYRSFIGLILIFAMTINVYLRKSDS"
     gene            6233..6973
                     /locus_tag="U6309_002275"
     CDS             6233..6973
                     /locus_tag="U6309_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEN5505709.1"
                     /translation="MINSHQTQTLLRLENIEKSYGSHRILNKVSFSLYPGEITALIGD
                     NGAGKSTLVQIISGYIQADKGNIYWQEEPLCYSNEYNAPFEMRSMGIQVVHQQLALID
                     ELSIWRNFFLGNEISKKIGPFKCLNIRLMQHIASQSLQSFGLVRPPNIDLPVKKLSGG
                     ERQILVICRAKYFNAKLLILDEPTSALSPAQADKVLQIIKNIAQAGIAIVFITHNPAH
                     VQNTAHKVINLSCGTVEIMELTNALSKG"
     gene            6977..7861
                     /locus_tag="U6309_002276"
     CDS             6977..7861
                     /locus_tag="U6309_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN5505710.1"
                     /translation="MKKLASTLLYFSLLISAAVFAGDKYYLITHGSQDPYWTSLFQGA
                     KKAAEELKIDLQILAPPGANDVPKQVQFIESALATHPSGIATTIPSDTAFSKSLQRAN
                     KLNIPVIAFDTKPKDKAKNPYMAFLGSDNQLAGKKLGEKAMELTPSAKRALVLNPQPG
                     HIGLEKRAQGIKTVLEDKGIFFEELDVGTDANQVQSRIKSYFKIHPETNIIFCLTSQA
                     LDPLGQMLLHPDRYDFNYQPQVYSFDKTPNTVSLIHKKLVNYVVDQQPFLMGYLSITQ
                     LVLMDRYQLNPVNINTAM"
BASE COUNT         2409 a         1593 c         1455 g         2598 t
ORIGIN      
        1 tgcagcgctg gttagaggaa atcaagagtc agggtttgaa ttttctattg caaagatttg
       61 ctgtctttgc acataaaatt caagccccgg caccattttt tccatctata aaaatagctg
      121 attattttaa ctgaggctta cctaagttag gttgaaccga gcttattgca tcctctgccg
      181 caagcacttc attgtcagta cttaagctta tgattttatt aattaattct cctggaagtc
      241 tgttgaagaa gcaagtgtta tttggatttc taagtgcttg gcccaaaatc cttgcagcat
      301 aacgaacatc cgcatgggca gcattagatg attgtgaaac acacgctggt gcattaatac
      361 caaagttata tccgggggta tgcaaatttt tatcattaaa atataccgca tcctgccctt
      421 cataaattga ccatgttgct gaagaggcgt gtctaagtaa agttaaaaag ttttgacgta
      481 tttccttatt aggaaaaact acggctaact ccccattttt tgcagtatag gtactgagat
      541 acagcgtagt gggattttca ggataagttt gcggacattt tttattggca ttgaatttat
      601 cagaagatgc aaaatgagat acgcccccca tcttttgaat aaatcggtct ctccattctt
      661 gggtaggaaa ctttagtgca agttctccat tttttgcttt ataaataata attttttctt
      721 ggcttatgca tgcatgaaaa ttttgtaaat ttgttaataa ttccgggtta tccttggaat
      781 agtgaataca cattttttgg aaatgatctt taaaaaaatc attaactaac aattccttaa
      841 gagctatttg tggatcagaa ttcaacgctt ttatactgct catgcctgaa gtagctacaa
      901 ctggtgcaaa ttgagtgttg tttgctatag ctttcagagc agccagagca ttgggtataa
      961 tttgcgcatc acaacgtaaa agattttgat aacttgttgt aataatattt tgcagctcat
     1021 ttaagtgttt ttcatacttg ttgagtaaag caagtgcttc tttttgggat atcatatttt
     1081 ttcctcaggt tttcacatgg ctcgaaattc taacagtaaa ttattaaatc aatattaaat
     1141 taattttgac ccaagacatt gtgttctttt tataaactat tacattcact cgcttcttgg
     1201 tattgcccat ccattcaaaa ccatatactt ctcaatagtt atctatggtc ttttaaagta
     1261 aaaaaatttg ctattccctg tcgctcacat gcgatcatcg catctgtttt atttatgcta
     1321 gttataggat ctaatcttca aaccagataa gatatactgt ctgtcccgtc cgattagaac
     1381 aattattaaa cagtattcta atcggataat catttgaatg gagttctgaa ttaattgaaa
     1441 aaggagtttg ttatgaaacc aggttattta ttaggcgctt tgcttttatc tccccttctt
     1501 actttcgcca attctgataa cagctcagat ggccttattt tttcgccatt gccgcagaat
     1561 aaaaataccg tagtcaggca ctatagcaat gaacaggaca tgtctaatct taaccagatg
     1621 actcaaagaa ctattgattt tccaactcaa attgttcgtg ttagcggaaa tcttacagga
     1681 ttggaactta gctgtgacga tgtagaaaat gaaatagatc aaatcttttc aaaaaaaatt
     1741 tctcctaatt tgtttacata caacacctat gtcagctgcg gcttcgatga acatgatccg
     1801 gaacagtatg cgacaaattt ctctattcag agttatttcg atccattaac agataacgct
     1861 gtggattatt taaaaaccta tctgaaagaa tataatggat acaatttatt caatactaca
     1921 acattacaaa ttgaaaatgc caaaggcatc attgtatcga tgaatcttaa tgcgggctta
     1981 aaaaacaacc ctgataaaac gccattcatg ctttaccgtc aagataggaa caatttttat
     2041 tttaaaagca attttgacat gagaaaggaa ttaattaccg atatttatca acgattttat
     2101 tctaacgatc ctgatatgat tctacccttt ttcgacaaat ggattttctc ctatgcgggt
     2161 tcagtttatt actccattct gaaggcatcc aattatcttg aattgcaacc tgaaagaatc
     2221 tttgtaatgg aaaatgaagg ggatatcttt gtctctgatt taagatatta tttcgccaat
     2281 ctttgtatga aaaggaatcc taataaacat tgcttgtaaa taattcaatt attgagccac
     2341 gggcttgtct ttaggcattc cacaatcgtg gctcttagga ttccaggcca tttttttgcg
     2401 caccgttatg caatcgcaat ttggcgcaga gtttgaattt gcatttgaag tattcgcttt
     2461 tcttgcatac aaaatttaaa cactgctcac attagagttt tgggtaagcc atagctattt
     2521 aaaaacaact gatgcaacac gcgccgtagg aatatcgccg atgaactcct ttcgaatgag
     2581 agatttcgca cgctcatcat cgaaaacgtc cttgatatcc ataattttcc cagctggaac
     2641 attttccctg tgcaacattt ggagaagctt gttactttgc catttcgctg tttgttcagc
     2701 gattaataaa gcgagctttt ctcggttcct taccctgttt tgattgtgct taaactcatc
     2761 attatccggc aaatcgttta gcccaaggac agcgcatagt tttgcaaaat gcctatcaga
     2821 acctatagcc aagatgataa gcgcagcatc agctgtacgg aacaattcgc catagggagc
     2881 aatatttgga tgcagtgaac cgatacgctg cggcacctgc ccagtcatca agtaattaga
     2941 tgcctgattg acaagactgc tgacagcagc atcataaagt gaaacagaga caaccttgcc
     3001 tttcccactc ttttcccgat tgtagagagc cagaaggata gcctctttca ggtggtgagc
     3061 tgcgaggaca tcgattagag caacaggcat tttaactggg ccgctgtcag gtgtcccatt
     3121 gattgacata aaaccgcttt caccctggag aacaagatca taggcaacgc gctcgctttc
     3181 ttcaccataa ccattgattt ttccgataat taggcgcggg tttatactga gaagatgatc
     3241 ggcttgtatt cgtagcttta tgtcatctcc ctttttaaaa ttcataatta aaatatctgc
     3301 ttctgcaata aggttaagga aacagagata atcctcttcc ttcatgagat ctaaaaataa
     3361 atagtccttt ccgaaattaa cactggcaaa ataagctgaa atctcagaat gaggatcctc
     3421 tcctgggagt ttccatgttc ttgtcacatc cttatgcaca ggatgttcaa ttttaacaac
     3481 tgaagcacct aactcggcaa aaaaagtgcc tacagagggc cctgcaagaa ccgtactcag
     3541 atcaatgacc tttaagccct tcaacatata tctgttttcc aaatctcaat aaacattcat
     3601 tgtttttcca agcttttaca ttgggaaatt ctgcaaaact gaccctaggg acctcaaatc
     3661 aatatcctgc ctgatttagt gaggtcccga ctggaaaaat catgcttaag ttacattgct
     3721 tgacagttgt ttcaccaaat cataaaggaa tgcatgagct tcataaaatg attcgacact
     3781 aatccgttca tcacgaccat gggctcggaa atcatcgcga tctaaaaata gccccatcac
     3841 tccataggta ggaattccag ctgatcgcag atagcgtcca tctgttgcgc cagtaaccat
     3901 aattggaatg gcaggaactt caggccaata agtttgagtt agatgagtaa ttgtttttag
     3961 tatttccggg ctaagtggag aacttgggcc tctggacaat ttgccaatgc gctttagtgt
     4021 aacctctgga tcgttaatag ctgtcttcaa agattgctca accatttcag gtgaatcctc
     4081 tgggagcaca cgacaattta tggtaacagc cgctaattgt ggtaaagcat tcattgcatg
     4141 ccctccttca agtaaggtag gggtacaggt tgttcgtaaa atagcattcc attgcggtga
     4201 actatcagct aaacgttgca tagcttgctt cgagcctttg cttgcttcaa tcatttctgt
     4261 tttcagaggc cctgtctcaa tagccgccat ttgtttaaaa taggccgcag tgacatcatt
     4321 ggtttttaaa ggaaaattaa attttgacag tcgctctaaa gccccggcaa gacgataaat
     4381 ggcattatct ttggttggca gcgaactatg ccccccttta ttacggacct caaggttgta
     4441 attgacaatg tatttttcac tcacctggat attttgtgat atttttttcc cgttagccaa
     4501 atcaccccat ccaccctcat taagagcgaa atctgcctcg attaaatcct tgtgattttt
     4561 aatcagccaa ctaatcccgt tgtatgggct gcttccttct tcatcagccg tcaaggcaac
     4621 aatgatatcg cgtttgggtt taaatccttc ctgcttatat tggattaagt tggctatcca
     4681 aatggccgct tgcgctttat catccaaagt gcctctgcca taaaaatacc catctttttc
     4741 agtcagttgg aaaggattca tgctccaatc ggaagctttg gcttcaacaa cgtccgtatg
     4801 cgcaagtaat aacaagggtt tcttttcccc agtaccgcga taacgtacaa ccagattggc
     4861 tttttgtgga ctggctccac caacgaaaat gtcctcgttg gaaaaaccgg ctgtctttaa
     4921 gtattttaca gccacctcaa ccagcggcgt tgttgaaccg gtagtaaatc ctgattgcac
     4981 ttcgataaat tgtttataaa tagagcgagc aagttgttgt tcatcctctg gacgagtagt
     5041 cttagcttct acagccccaa caatcaatgc caaaatcata aaaaatgaaa tttgagttat
     5101 ctttttccta atcataaaat gcaatccatt caaagaatta agcgtttcaa aagaaactta
     5161 ctataaagta atctgagtaa aaatcaatgc gcgtatgggt tgaacttatc tctccatttg
     5221 actctgaaaa ttgtgcttaa aattcgtcaa tgttttatgt acactttcca cactaaattc
     5281 ccggggatag tctcatgtat aaaatacacc attatttttt atttggtgtt cttgttccat
     5341 ttattggcct tgttagtctg ttcaccattt tctcttctga ctttttaacc cttactactt
     5401 gggcgagtat tttggcatct gcctcagata ttggtatcgt tgtccttggc gtgactcttt
     5461 tgcttatagg aggagaattt gacctctctg ttggagcaaa ttttgcttta tctggcttgt
     5521 tgttcggttc actttcgtta ttaggaatca atagtatttt agctttaata attgctttga
     5581 caattggggc ctgcatagga ttggttaatg ccgcatgcac cgtctttttt aaaatcccct
     5641 cttttattgt gactttggga agcatgttaa ttttacgtag ccttattctt ttatacaccc
     5701 aaggtttgcc catttctatt gaatatacca atgttgttat gcaagtatta gcaggtgaat
     5761 tatcgcaaaa cttattgtct tcttttattt ggtggattgt tattggcttt tatctgatct
     5821 atcttctcaa ttggacaaaa ctaggcaatc atataattgc cactggcaac aattgtgatt
     5881 ccgcttattt atccggcata aataccatgc gagttaaatt cttttgtttc gtctgttgtg
     5941 gaataattgc agcatttgcc ggagtaactc aatttagtca tcttaatagt ttatctccga
     6001 tcgcaggcga gcaatatgaa ttatatgcca tagcagctac cgtaattggt ggtacctcac
     6061 taaaaggagg tcaaggatcc ataataagca cactactggc tactttatta ttaagcactg
     6121 tagatacggg catagtacaa attggcgttt caacttattg gtatcgatct tttattgggc
     6181 ttattttaat ctttgcgatg actattaatg tttacctgcg gaaatctgac tcatgataaa
     6241 tagccatcaa actcaaactt tactccggtt agaaaatatt gaaaaaagtt acggctccca
     6301 tcgtattcta aataaagtat cctttagttt atatcctggg gaaattactg ctttaatcgg
     6361 tgataatgga gcaggtaagt caaccttggt gcaaataatt tcaggttata ttcaagcaga
     6421 taaaggaaat atttattggc aggaggagcc attatgctac agcaacgagt ataatgctcc
     6481 atttgaaatg agaagcatgg gtatacaagt tgtccatcaa caattagcgc ttattgatga
     6541 attatctata tggcgtaatt tctttttagg taatgaaata agtaagaaaa taggtccctt
     6601 caaatgttta aacatcaggc ttatgcaaca tatagcaagt cagtccctgc aaagctttgg
     6661 ccttgtacgc ccgcctaata ttgatctgcc ggtcaaaaaa ttatctggtg gtgaacgcca
     6721 aattctagtc atttgtcgcg caaaatattt taatgcaaaa ctacttattc ttgacgaacc
     6781 aaccagtgcc ctctcacctg cgcaagcaga taaagtcctt cagatcataa aaaatatagc
     6841 tcaagcagga attgcaattg tttttataac tcataatcca gctcatgtac aaaataccgc
     6901 acacaaagtg atcaatctca gttgcggaac agtagaaata atggaattaa ctaacgcgct
     6961 aagtaagggt taaactatga aaaaattggc atcaactctt ttgtatttct cgctattgat
     7021 aagtgccgct gtcttcgcgg gagataaata ttatttgatt acccatggtt cacaagatcc
     7081 ttattggaca tctttatttc aaggggcaaa aaaagctgct gaagaactga aaatcgattt
     7141 acaaatttta gctcctccag gagctaatga tgtgcccaaa caagttcaat tcatagagag
     7201 tgcactggct acccatcctt ccggaattgc tactaccatt ccttcagaca cagcttttag
     7261 caaaagcttg caaagggcaa ataaacttaa tattcctgtt attgcctttg ataccaaacc
     7321 aaaagacaaa gcaaaaaatc cttacatggc ttttttaggt tctgataatc aactcgctgg
     7381 taagaaactt ggtgaaaaag ccatggaatt aaccccaagc gctaaaagag cactggttct
     7441 taacccacaa cctggccata tagggctcga aaaacgtgcc caaggaatta agaccgttct
     7501 tgaagataaa ggaatctttt ttgaggaact tgatgtaggt actgatgcga atcaagtaca
     7561 atctagaatc aaatcctact ttaagataca tccagaaacc aatattattt tttgcttaac
     7621 ctcacaagcg ctagatccgc ttggccaaat gttattacac cctgacagat atgattttaa
     7681 ttatcaacct caagtctatt cctttgataa aacacccaat actgtttctt taattcataa
     7741 aaaactggtg aattatgtcg tggatcaaca accattttta atgggttatt taagtatcac
     7801 tcaactggta ttaatggacc gatatcaatt gaatccagta aatatcaata ctgcaatgta
     7861 aattgcagta taagtgttat atttcagcca acggcttgtt gctttttgat agtatcatgg
     7921 ataatttcgg gcacttccag gaagctagtt gcgatcaaaa ttgcaagaaa attaggtata
     7981 aaccagttta tattggaatg agatgtgaac gactaattca agagggcaaa ctcgattgcc
     8041 ctgaatatcg ctctc
//
LOCUS       DASLOE010000134         8035 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.135, whole genome shotgun sequence.
ACCESSION   DASLOE010000134 DASLOE010000000
VERSION     DASLOE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.135"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            4..681
                     /gene="trmB"
                     /locus_tag="U6309_002277"
     CDS             4..681
                     /gene="trmB"
                     /locus_tag="U6309_002277"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEN5505711.1"
                     /translation="MQRKIKSYVLRAGRISNRQQQGLDLWLEDYELKFDSPSPWNFAK
                     EFGRHDADTIVEIGFGMGTSLFTMAMNNPQCNYLGIEVHKAGVGSLVADLHEHQITNV
                     RVVVHDAVDVLQTKIPENSLAGVQIFFPDPWHKKRHHKRRLIQSEFIQMLVKKIRPSG
                     FIHCATDWEDYAEHILNVLSSESALFNQQKEGGYSPRPDSRPLTKFEQRGERLGHGVW
                     DLVFIKK"
     gene            693..1817
                     /locus_tag="U6309_002278"
     CDS             693..1817
                     /locus_tag="U6309_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEN5505712.1"
                     /translation="MIKHAILISGLIVSFLASAAEMSFLAQLTEVPGASGYEKNVRHL
                     VQKQWQSYMAELKIDGMGNLIGQRKDNQKGPKLLFMAHLDETGFMVESITPDGFLKVI
                     PLGGIANSVIYAQRWTVSTPKGPVLAYSGMDSPHLLGDKKMSGSPDINALFLDIGAEN
                     KKQAQKEFAIQPGLEVTPVSEFSQLSKNRFLGKALDDRIGLAVISDALRAVKQQPNQL
                     YLAATVQEEVGMRGASTVYKATHPDIAINVEVGIADDYPMLLADRKGRIGLGKGPSLF
                     VYDKSMIPNQELINWIMELANKNNIPLQLEVEPGYGEDGSKVQASGMGVPVVNIGIPI
                     RYAHQHAGIFDKRDYDNTVKLLRLIAENLNQEVVDKIKKS"
     gene            1895..3382
                     /gene="ankC"
                     /locus_tag="U6309_002279"
     CDS             1895..3382
                     /gene="ankC"
                     /locus_tag="U6309_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkC/LegA12"
                     /protein_id="HEN5505713.1"
                     /translation="MDFVSEMNKILQMELEQFKEFIQKKLEEDKNYLEKLFWLPNGSQ
                     MTVLNYLIEQYSEPKLGIDDANRDLLAKIDFVLHEAKDVNVGEPLHQAIVAGKISLAL
                     HLLGVDENNFTPGESEKTDVLSILSKVKKKIEESFNHVKRCFFDVDKRDGYGRTLLSL
                     ALDAKRQELLIAILARNPIVHATTLRSSAYVPFQPIHQAVVLDYAEGITLLASMGAQL
                     TNPLGSMRDTPVILAARLGKINALAALLELPTQSLSLESENNHLFEDKQTGHTAVEEL
                     CERMANENDKAEALRGIAMLICRGAEPPRNEKMRNLLSSNRVAFLKAVSTYLADKPQL
                     VDAFVERCHLRESALHNIVYADHSWGSSIRHLLGVPSEAALIVEELVTRKYSDPSFAS
                     ENVSSLSTTATENLRSERNPLKLYAEFVRRYTQAYDSQMITNRWSTMRWMIAEGNCDW
                     DTVVRYSKNHPTSRTRIVLNEMFNPIPRVHEDIESQQNSPRVVLK"
     gene            3591..4733
                     /gene="hflK"
                     /locus_tag="U6309_002280"
     CDS             3591..4733
                     /gene="hflK"
                     /locus_tag="U6309_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEN5505714.1"
                     /translation="MGWNEPEKGKDPWSGKNQPPDLDEALKRIQDKLKKTFFGGTGKS
                     NNNSSGGSSGGLLAVTVLLIAFILWALSGIFIVDPAEQAVILRFGKYAETVGPGPHWI
                     PRFISSKIVMNVDRVLDYSYSAQMLTSDENLVSVSLAVQYRINDLSEYLFNVANPEES
                     LQQATSSALRQVVGTTTLDQIITEGREVWGGRVQETLTKTLESYKTGILIVNVSPQPA
                     RAPESVQDAFDDAIKAQEDEKRFKEQAYAYAAKVVPIAEGKASRIQQEAEAYSKQVVL
                     RAQGEVAEFLALLPQYNAAPQVTAKRMYLEAMQKVMNKSSTIIVDSKAGNLLYLPLDK
                     LLGNQYVPQSGNLKAIKNGTNVSNEEDNLILSGRDLTRPTYSQGRD"
     gene            4736..5650
                     /gene="hflC"
                     /locus_tag="U6309_002281"
     CDS             4736..5650
                     /gene="hflC"
                     /locus_tag="U6309_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEN5505715.1"
                     /translation="MNAFKTTLGVLIFLVLVLLFTTMFTVTQGQQGIILRLGRLVKDP
                     QTDAVKVLNPGLHFKTPFIESVRIFDTRIQTMDIKSTRIVTKEKKDVMVDYYVKWRIS
                     DLAQYFKSTGGNEFKAETLLEQQLNTLLRAQFGKRTISDAVSGGRDDVMEILRNAAEK
                     QAGELGIKVVDVRIKGIELPSNTSNAIYQRMRADMQKIANRHRADGQAAAEQIQAKAD
                     ADVTVLLAKTNSNAQRIRAVGEAEAAAIYSKAYTQNPDFFALYKSLLAYEASFHSKKD
                     ILILDQSSSFFDYFKQAMPKNDGTAAKK"
     gene            5785..7080
                     /locus_tag="U6309_002282"
     CDS             5785..7080
                     /locus_tag="U6309_002282"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEN5505716.1"
                     /translation="MGKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTL
                     KINGVKTVLRLIPSGMLRPNVTCYIANGVVLSPQALLSEIKELEGNGVNVRERLRISL
                     ACPLILPYHIALDKARETHMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFAN
                     KLTELLDYHNFVLTQYFKQPAVDLESLLDESLQWVEELRPMVCDVSACLHEHRKQGEN
                     ILFEGAQGVYLDIDHGTYPYVTSSNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGG
                     GPFPTELLDDVGKRIAERGQEFGAVTGRPRRCGWFDAVLLKRSIELNSISGLCVTKLD
                     VLDGLEVLRIAVAYKDRDGNILSRPPLAADDFNDLLPVYEELPGWQESTADVTLMSDL
                     PANARAYLKRIEEILGIPIDMLSTGPERDSTITLRGPFL"
     gene            7300..7869
                     /locus_tag="U6309_002283"
     CDS             7300..7869
                     /locus_tag="U6309_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEN5505717.1"
                     /translation="MEYLTPSGLIPFITELKALNILQQDDNTNIALVSEILARWVVDK
                     EWLDEKFYYVNPEVGFNSWLIHEEKDHTLAVNIVAWQSGREITPHDHQTWGVVGSIIG
                     VEKNYFWKRMDDGSKPGYAEIIREKTAVIRPAGGVIGFLPNDIHSVMNESDDVAISLH
                     VYGKNLNYTGRHQYDPVNNLMQPFIVNYS"
BASE COUNT         2460 a         1388 c         1792 g         2395 t
ORIGIN      
        1 gtaatgcaac gtaagattaa aagttacgtg ttgcgcgctg ggcgaatcag taacagacag
       61 caacaaggat tagatctttg gcttgaagac tatgaattaa aatttgatag tccctctccc
      121 tggaattttg caaaggaatt tggccgtcat gatgcagata ctattgtaga gattggtttt
      181 ggtatgggta cctcgttgtt tactatggca atgaataacc ctcaatgtaa ttatctgggc
      241 atagaagtac ataaagcagg tgttggtagt ctggtagcgg atttgcatga gcaccagata
      301 actaatgtgc gggtagtagt gcatgatgca gtagatgtat tacaaaccaa aataccagaa
      361 aactcacttg ccggggttca aattttcttt cccgatccgt ggcataaaaa gcgtcatcat
      421 aagcgacgac tgattcaatc cgaatttatt caaatgttag taaaaaaaat caggccatcg
      481 ggatttattc actgtgctac tgattgggaa gattatgcag agcacatttt aaatgtttta
      541 tcttcagaat ccgccttgtt taaccaacaa aaagagggcg gatactcacc acgcccggac
      601 tcaaggccgt taacaaaatt tgagcagcgt ggtgagcgtc taggccatgg ggtatgggat
      661 ctggttttta taaaaaaata aggaagttac ctatgataaa gcatgcaatt cttattagcg
      721 gattgattgt ttccttttta gcttcagcag ctgaaatgtc gtttctagcc caattgacgg
      781 aagtacctgg cgcctctggg tacgaaaaaa atgttcgtca tttggtacaa aaacagtggc
      841 aatcctacat ggcggagcta aagatcgatg gtatgggtaa tttgatcggc cagcgtaaag
      901 ataatcagaa aggaccgaaa ttactattca tggcacatct tgatgaaacg gggtttatgg
      961 tggaatccat aacacctgat ggctttttga aagttatccc tttaggcggg atcgcaaact
     1021 cggtcatcta tgctcaacgc tggacagttt caacacccaa aggacctgta ttagcttatt
     1081 ctggcatgga ttctccgcat ttattgggtg ataagaaaat gtcagggtct cctgatatca
     1141 atgccctgtt tttagatatc ggagcagaga ataaaaaaca agctcagaaa gaatttgcaa
     1201 tccaacctgg ccttgaagtc acaccagtaa gtgaatttag ccaattaagc aagaacagat
     1261 ttttaggcaa ggccttggat gatcgcatag gattagctgt cattagtgac gctctgagag
     1321 cagtgaaaca acaacccaat caattatatc ttgctgccac agtgcaagag gaagttggta
     1381 tgcggggtgc cagtacagta tacaaagcga ctcatccaga tattgcaatt aatgttgaag
     1441 tgggaatcgc agatgattat ccaatgctgc tggctgatag aaaaggtcga attggtttgg
     1501 ggaagggacc atccttgttt gtttatgaca aatcaatgat tcccaatcag gagttaatta
     1561 actggataat ggaacttgct aataaaaata atatcccgct acaacttgag gtggagccag
     1621 gatatggtga agatggctcc aaggttcagg catcaggcat gggcgttcct gtcgtcaaca
     1681 ttggtatccc tattcgatat gcacaccaac atgccgggat atttgataaa agagattacg
     1741 ataatacagt taaattgttg aggttgattg ctgagaattt gaatcaggaa gtggttgaca
     1801 aaattaaaaa gagttaacaa tattttgatc tcccattgtc cattgtgtaa tattagtgtc
     1861 ctataaatag tttataaata aaggaagtat tattatggat tttgtaagtg aaatgaataa
     1921 gattctacaa atggaattag agcaatttaa agaatttatt caaaagaagc tggaagaaga
     1981 taaaaattat ttagaaaagt tattttggtt gccgaatggt tctcaaatga ctgttttaaa
     2041 ttatttgatc gaacaatact ctgagcctaa gcttggtata gacgatgcaa atagggattt
     2101 gcttgccaaa atagattttg tccttcacga ggctaaagat gtgaatgttg gtgagccttt
     2161 gcaccaggca attgttgctg gaaaaatttc cttggcactc catctgctgg gagtggatga
     2221 aaataatttc acacccgggg agagtgaaaa aacggatgtt ttgagtattt tgtctaaagt
     2281 aaagaaaaaa atcgaagagt cttttaatca cgtaaaaaga tgtttttttg atgtggataa
     2341 acgggatggt tatgggcgca ctttgttgtc tttggcatta gatgccaaac gccaggaact
     2401 gttaattgcc attttagcaa ggaatcccat tgtacacgca acaacgttaa gatcctcagc
     2461 ctatgttcct ttccagccta ttcatcaggc tgttgtttta gattatgcag agggcattac
     2521 cttattagcc agcatggggg cgcaattgac aaatcccctg ggtagtatga gagacactcc
     2581 ggtcattttg gctgctcgtt tgggcaaaat aaatgcactg gcggctttac tggagttacc
     2641 cactcaaagt ttgtctcttg aatccgaaaa caatcattta ttcgaagaca aacaaactgg
     2701 ccatacagca gtggaagagt tatgtgaacg catggccaat gagaatgata aagcagaggc
     2761 tcttcgtggg attgccatgc taatatgccg tggtgctgaa ccacctcgca atgaaaaaat
     2821 gcgaaattta ttaagcagca accgggttgc tttcttaaaa gcggtcagta cctatttggc
     2881 agataagcct caattggtcg atgcttttgt agagcgttgt catctacgag aaagtgcttt
     2941 gcataatata gtttatgccg atcattcatg ggggagttct attcgtcatt tactcggtgt
     3001 tcccagtgag gcggccctta ttgttgaaga attagtaacg agaaaataca gtgatccttc
     3061 ctttgccagc gaaaatgttt cgtcattatc aactactgct acggaaaatc tacgttcaga
     3121 gaggaaccca ctgaaactct atgccgaatt tgtcaggcga tacacgcaag catatgatag
     3181 ccaaatgatt accaatcgtt ggagtaccat gcgctggatg atcgccgaag ggaattgtga
     3241 ttgggataca gtagttcggt attcgaaaaa tcatccaacg agccgtacca ggattgtgtt
     3301 gaatgaaatg tttaacccga taccgagggt acatgaggat attgagtctc aacagaattc
     3361 tccaagagtt gttctaaaat aatttatgtc aatattttga tcttgaacct tcgacagaca
     3421 ccttggcggt ctgcgaaggt ttataaaaca tcttgagtat ttccagatga ttgccaagta
     3481 cagatagtct tttgcttcat ttcaatatac aagtcctgaa gtatctactt gcttaaactt
     3541 catatacccc ataaaatcct aaattcattt gaaattgcta ggagtagtac atggggtgga
     3601 atgagccaga gaagggcaaa gatccatggt cgggtaaaaa tcaacctccg gatttggatg
     3661 aagctcttaa acgtatccag gataaattga agaaaacatt ttttggaggc acgggcaaat
     3721 caaataataa ttcgtcaggc ggttccagtg gcggattgct tgctgtcaca gttttattaa
     3781 tagcttttat tctttgggct ctttctggta tttttattgt ggatcctgca gagcaagctg
     3841 tgatactccg ctttggtaaa tatgcagaaa cagttggtcc tggtcctcat tggattcctc
     3901 gttttatctc atcaaagatt gttatgaatg tggatagggt gctagattac tcctactccg
     3961 ctcaaatgct taccagtgat gaaaatttag tatcggtctc tttggctgtc cagtaccgca
     4021 ttaatgattt gagtgagtat ttgtttaatg tcgcaaatcc tgaggaaagt ttacaacagg
     4081 caacttccag cgctttaagg caggttgtag gtacgacaac cctggatcag ataataactg
     4141 aaggtcgaga agtttgggga ggtagagtac aagaaactct gacgaaaact ttagaaagct
     4201 ataagaccgg aattttaatc gttaatgtgt caccccaacc tgccagagct ccagaaagtg
     4261 ttcaggatgc ttttgatgac gcgattaaag ctcaagaaga tgaaaaacgt ttcaaggaac
     4321 aggcttatgc ttatgcagca aaagtcgtgc ccattgcaga aggtaaagca agtcgtattc
     4381 aacaggaagc ggaagcttat tcaaagcaag tcgttttaag ggcacaggga gaagtagcgg
     4441 agtttttagc cttacttcct caatacaatg cagctcctca agttacggct aaacggatgt
     4501 accttgaagc catgcaaaaa gtaatgaata agagcagtac gattattgtt gacagtaaag
     4561 caggaaattt attgtatcta cctttagata aactacttgg taatcaatac gttccgcaat
     4621 ctggaaatct aaaagccata aaaaacggta cgaatgtcag taatgaggag gacaacttga
     4681 ttctttctgg aagagatctc acaagaccaa cttatagtca agggagagat tgagaatgaa
     4741 tgcatttaaa acgacattgg gtgtcttaat ctttttggta ctagtgcttt tgtttaccac
     4801 tatgtttact gtcacacaag ggcagcaggg tattatttta cgtttaggac gtttggtaaa
     4861 agaccctcaa actgacgcag tgaaagtgtt aaatcctggg ctccacttta aaaccccttt
     4921 tatcgaaagt gtgcgaattt tcgatacacg catccaaacc atggatatca aatcgacccg
     4981 tattgtgacc aaagagaaaa aagatgtcat ggttgactat tatgtgaaat ggcgaatttc
     5041 agatttggct cagtatttta aatcaacagg tggaaatgaa tttaaagctg aaactttgct
     5101 agaacaacag ttaaatacct tgctcagagc tcaatttggt aagcgtacca tatccgatgc
     5161 tgtttcagga gggcgtgatg atgttatgga aatactgcgt aatgctgctg agaagcaagc
     5221 tggtgaatta ggtattaaag tggttgatgt tcgtattaaa ggtattgaat tgccttcaaa
     5281 taccagtaat gccatctatc aacgcatgag agctgacatg caaaaaattg caaacagaca
     5341 tagagctgat ggacaagctg ccgcagaaca gatccaggca aaagctgatg ctgatgtcac
     5401 ggttttattg gctaaaacta atagtaatgc tcagcgaatt agagcagttg gtgaagcgga
     5461 agcagcggct atctattcca aagcatacac tcaaaatcct gatttttttg cattatacaa
     5521 aagcttgtta gcctatgaag ccagttttca tagtaaaaaa gatattttaa tattggatca
     5581 aagcagttca ttttttgatt atttcaaaca agctatgcca aaaaatgatg gtacagcggc
     5641 taaaaagtaa ttataatgtg aaattttgct gtaatcaggt tcgatattta ttagcatcat
     5701 ccatctaata taaatgcagt tgggtttctc gtaaaataca actgcatttt taatttttac
     5761 aggtatgcaa acaaagagta aatcatgggt aagaatgttg ttgttttggg tacacaatgg
     5821 ggagatgaag gcaaaggcaa aattgttgat ttgctgacac aagatgctca agtggttgtc
     5881 cgttatcaag ggggccataa tgcaggccat acattaaaaa tcaatggagt aaaaacagta
     5941 cttcgtttaa ttccatccgg gatgcttcgc cctaacgtaa cctgctatat tgcaaatggt
     6001 gtggtcttat cacctcaggc attacttagt gaaattaagg agcttgaggg taatggcgtt
     6061 aatgtgaggg agcgattacg cattagcttg gcttgtcccc tgatcctccc ttaccacatt
     6121 gctttggata aggctcgtga gacacatatg ggcaagtctg caattggaac aacaggtcgt
     6181 ggtataggtc ctgcttatga ggataaagtg gctcggcgcg ctttaagagt aggtgattta
     6241 tttcatagag accgatttgc caataaatta acagaattat tggattatca taattttgta
     6301 ttaacacaat atttcaaaca accagctgtt gatttggaat ccctattgga tgagtcatta
     6361 caatgggttg aagaattaag accaatggtt tgcgatgttt ctgcctgttt gcatgaacat
     6421 agaaaacaag gtgaaaacat tttattcgaa ggggcgcagg gtgtttattt agatattgat
     6481 catggaacat acccctacgt gacttcttca aatacctgtg tgggatctgt aattaatggt
     6541 gcgggatttg gtcctcgcta tattgattac gtgttaggta tcacgaaagc ttacacaact
     6601 cgtgttggtg gtggcccatt ccccactgag ttactggatg acgttggaaa acgcattgca
     6661 gaacgaggtc aggaattcgg tgcagtaact ggtaggccca gacgttgtgg gtggtttgac
     6721 gctgttttat taaagcgctc gattgagtta aatagcattt ctggactctg cgttaccaaa
     6781 ttggatgtgc ttgatggttt agaggtattg cggattgctg tggcttataa agacagggat
     6841 ggcaatatat tatctcgacc tcctctggca gctgatgatt tcaatgattt gctaccagtc
     6901 tatgaggagc ttcctggttg gcaggaatct actgctgatg tgactttgat gagtgactta
     6961 cccgccaatg cacgggctta tctaaagcga atagaagaga tattaggaat tcctattgat
     7021 atgctatcaa caggacctga aagggattca acaattactt tacgaggtcc ttttctttga
     7081 tacaaagata aagttttgta accactatga gtgtcatgca aggatttctt gatatgacac
     7141 tttccgcctt ttaagtatta ttgtctgtgt tttagcaagc tatttccttg gggcagcagg
     7201 caattcttgc atcgctaaat ttcatttcat gctaaagtta atgtacataa aagataaatt
     7261 atctcttatc tgaatgttta ctcaataaaa tggtgaatta tggaatatct aactccctca
     7321 ggattgattc cttttataac tgaactcaaa gcgcttaata ttctacaaca agatgataat
     7381 actaatattg ctctcgtatc agaaatttta gcgcgctggg ttgtagataa agaatggctg
     7441 gatgaaaaat tttactatgt caatccagag gttggcttta actcctggtt aattcatgaa
     7501 gaaaaagatc atactttagc agttaatatc gttgcctggc agtcaggacg agaaattact
     7561 ccgcatgatc accagacttg gggtgttgtg ggtagcataa taggtgttga aaaaaattat
     7621 ttttggaaaa gaatggatga tggttcaaag ccagggtatg ccgagatcat aagagaaaaa
     7681 acggctgtta tacgtcccgc tggtggggta attggttttt tgcctaatga cattcatagt
     7741 gttatgaatg aatcagatga tgtagcaatt tcactgcatg tttatggaaa aaatttgaat
     7801 tatacaggtc gtcatcagta cgacccggtt aacaatttaa tgcaaccatt tatagtaaat
     7861 tatagttagg ttgatattta tcattgtcgc tatgctttca atcctgccaa attgctgttt
     7921 actcacacag ggcttgtaaa ttgatattaa actctttgtt aaaatatatt tttaattctg
     7981 tcatttagtt tatctaaatt ccctcattaa aaacatgttt tacttcaagc ttggg
//
LOCUS       DASLOE010000135         7911 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.136, whole genome shotgun sequence.
ACCESSION   DASLOE010000135 DASLOE010000000
VERSION     DASLOE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.136"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            140..277
                     /locus_tag="U6309_002284"
     CDS             140..277
                     /locus_tag="U6309_002284"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN5505718.1"
                     /translation="MTNVVLRTDISWRDVPVCYGKWHTIYTRFKRWSENGLFLFLVYQ
                     L"
     gene            414..665
                     /locus_tag="U6309_002285"
     CDS             414..665
                     /locus_tag="U6309_002285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505719.1"
                     /translation="MACLSANNTIDMKEFTVFWQKCDWQGINYIVANKGYDYSAVRNQ
                     ICTADRIPVIPRKKGAICPVVRDKEGYKTRSAIERLLEN"
     gene            complement(873..2654)
                     /gene="ppgA"
                     /locus_tag="U6309_002286"
     CDS             complement(873..2654)
                     /gene="ppgA"
                     /locus_tag="U6309_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector PpgA"
                     /protein_id="HEN5505720.1"
                     /translation="MKEPRELNRQPNPEHMAQLIKDTPKDVWFHLAKYLPDHELLNLM
                     QVDKRLNRFFKEGKPPKEIIKKIEHAPLKVVSSGVNTFILTQYHGKPLLLACGQNKDG
                     QLGCGDTKIRTTLTPVNIPESFASIDSVQISLFHAVLWGRDKDNKPILATCGLNDKGQ
                     LGCGDYKSRALFTQVKLAEEITSIESVEINIFNSVILGHDKHNRPVLASCGSNHLGKL
                     GCGDIKDKTTFTRMKLPTEIVSIDTLQMNWHTIVSGRDKNNKPVLATCGWNDRGQLGC
                     GDKKERALLTPVKLPENIVSIQSAQIGTSRTAVLGRDKDNKPILIYCGWNERNQMGWS
                     NTEDKTALTPAKLPEDIVSIKSMQISTSRTVILGRDKNNKPILAACGLNNHGQLGCGD
                     IKTRTTLSQIKFPKDIVSIESVQISDNHTMILGRDKHKNPIIAACGLNNYGQLGCGDT
                     KNRMLLTLIKLPKEMVCIDSITVKYNCLFVSGRDKNHRPILTACGDNRYGQLGIPGKK
                     LLTELTIVPTPILTLPATLKREGSSRKGATEKALVFAAHGSGEQKSQKSNRILTAIIA
                     SFGRFFTGAHPHTPDSDKESPSPKRSA"
     gene            complement(3087..4745)
                     /locus_tag="U6309_002287"
                     /pseudo
     CDS             complement(3087..4745)
                     /locus_tag="U6309_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrB"
     gene            complement(4880..6664)
                     /locus_tag="U6309_002288"
     CDS             complement(4880..6664)
                     /locus_tag="U6309_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrB"
                     /protein_id="HEN5505721.1"
                     /translation="MKEPRELNRQPNPEHMAQLIKDTPKDVWFHLAKYLPDHELLNLM
                     QVDKRLNRFFKEGTPPKEIIKKIEHAPLKVVSSGVNTFILTQYHGKPLLLACGQNRDG
                     QLGCGDTKIRTTLTPINIPESFASIDSVQISRYYTVLWGHDKHNKPILAACGENDQGQ
                     LGCGDTTIRTAFTTVKLPEDIVSIKSVQISDYHTIILYHDKYNNLMLAACGLNNYGQL
                     GCGDTENKTQLTPIKLPKNIVYIKSVQINISCTLILGQDRQKQSILASCEVNHCDYVS
                     CNGIENKTSITPVKLPEKIFSIESVQISGNHTVVLGRDEHNKPILAACGLNNHGQLGC
                     GGYTYKSSLTLVKLPEHITTIELMQISSSRTVILGHDKRNKPMLAACGANYSGQLGCG
                     DTENKTLLTPVKLPKNISYIQSVQISDDYTVVLGRDKYKNPIIATCGLNNYGQLGCGD
                     TKNRMLLTPIKLPKEMVCIDSMTVRHRSLFVSGRDKNHRPILAACGDNRYGQLGVPGK
                     ELLTELTIVPTPILTLPATLKREGSSRKGATERALVFAAHGSGEQKPQKSNRTLTAIV
                     ASFGRFFTGAHPHTPDSDKESPSPKRSA"
     gene            complement(6739..7680)
                     /locus_tag="U6309_002289"
     CDS             complement(6739..7680)
                     /locus_tag="U6309_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-box protein"
                     /protein_id="HEN5505722.1"
                     /translation="MRYKFEFFGKLLSELKIEIARTLPKNDLAKLVESSKIHLKLFKP
                     LLDVQKLLHHTVRGEHDAVQSMLKKDINLFFMKGRVTDCSGRTFENISAFEYTLWALD
                     KYMWEKMLPCIPKTEKGKKILAELLFQYDEVNTNGVTYKLHGKPIREKHFDFKNTLIK
                     ELQIQLDLLETPEDDKNWDVIDKQWREGVGAAQKLLPMHVVHEYCSDEVFHPPLEFIS
                     QPKSSMQFHIGRSEKSENWFGIDSRLGINYAVYCGRPGLGGARSPSAPAFRIPWIGEN
                     LTAVKTLCEIRTKDFINLRSNLEQQTIIDALHRVYRK"
BASE COUNT         2089 a         1475 c         1741 g         2606 t
ORIGIN      
        1 atcgtaatat ggcactgcta acaaagtatg gtgatgtggt ataaagagca atttattaag
       61 agtgcagtaa tggacgcaag gctttaccgg ctaaaagaag aagattttat aagagatata
      121 gcaccctata tagccaagca ttacaaacgt agtattaaga acagacatca gttggcgaga
      181 tgttcctgtc tgctatggaa aatggcatac catttatacg cgatttaaac gatggagtga
      241 gaatggattg tttttgtttt tggtttatca actttaacaa aagaaaagat aactgtggat
      301 attagctggg tatacagcac gaatattggt attcagcgtc atggctcagg cgtattaaaa
      361 aaagtacgca aactataggg tgaggtcaca aaagagtagg aaccaaaatt caagtggcct
      421 gtttatcagc aaataacacc attgatatga aagagtttac tgtgttttgg cagaaatgcg
      481 attggcaagg tatcaactat attgttgcca ataaaggata tgattattct gcggtaagaa
      541 atcaaatctg tacagctgac aggataccag ttattcctcg taagaaaggg gctatttgcc
      601 ctgtggtgcg agataaagag ggctataaaa ctcgttctgc tattgaacga cttttggaaa
      661 actaaaataa aataaacgtc tcagcttacg gtttgataaa ctggatgtaa ttttcttttc
      721 ttttttgctt taggctgcct taaggcgctc aatttacttt gttaacagtg ccatatcaaa
      781 caataggaca aatggaattc aaaaaacaat caattgatga acgagcataa gaggactttt
      841 gggtcctctt gcttctaaag ttcttgtttt gcttaggcgc ttcgtttggg tgaagggctt
      901 tctttatcgg aatccggagt gtgaggatgt gctccagtaa aaaaacgacc gaatgatgca
      961 atgattgcag tgagtatcct gttgcttttt tgggattttt gctctccgct gccatgggca
     1021 gcaaagacta gagctttctc tgtagcccct ttcctggaag aaccctctct ttttagtgtt
     1081 gcaggcaatg tgagaatggg ggtgggaacg atggtcagtt cggttagaag tttcttgcct
     1141 ggaatcccca gttgaccgta gcgattatcc ccgcaagctg tcagtatggg acgatggttt
     1201 ttatcacgac ctgaaacaaa aaggcagttg tattttactg ttatggagtc tatgcaaacc
     1261 atctctttgg gcagtttgat gagtgtgagt aacattctat tttttgtatc cccacagcct
     1321 agttggccgt aattattaag cccacaggct gctattattg gatttttgtg cttatctctt
     1381 cctaaaatca tcgtatggtt atcactgatt tgcaccgact caatagaaac gatgtctttg
     1441 gggaatttga tttgagaaag ggtcgttctg gttttaatat caccgcaccc aagttgaccg
     1501 tgattattta gcccacaagc agccaatatt ggtttgttgt ttttatcgcg acctaaaatt
     1561 acagtgcggc tagtactgat ttgcattgac ttaatggaaa cgatgtcttc aggtaatttt
     1621 gctggggtga gtgctgtttt atcttcagtg ttactccaac ccatttggtt tctttcattc
     1681 catccacaat agatgagtat cggcttattg tctttatcgc ggcctagaac tgcagtacga
     1741 ctagtgccaa tttgtgctga ttggatggaa acgatgtttt caggtaactt gactggggtg
     1801 agtaatgctc tttctttttt atccccacac cctagttggc ctctatcatt ccatccacaa
     1861 gtagccagta ctggtttgtt attcttatcg cgtcctgaaa caattgtatg ccaattcatc
     1921 tgtaaagtat ctatggagac aatttctgta ggcaatttca ttcgggtgaa tgttgtttta
     1981 tcttttatgt ctccacaacc taatttaccc aagtgattgc ttccgcagga tgcgagcact
     2041 ggtctgttat gtttatcatg gcctaaaatc acagaattga aaatgttgat ttctactgat
     2101 tcgatggaag tgatttcttc tgctaatttg acttgggtga ataatgctct agacttatag
     2161 tctccacaac ccagctgacc tttatcattt aatccgcaag tcgctagtat cggcttattg
     2221 tctttatcac gtccccaaag cactgcatgg aaaagactga tttgcaccga gtcgatggac
     2281 gcaaatgatt cagggatatt gacaggggtg agtgttgttc taattttcgt atccccacaa
     2341 ccaagctgac catctttgtt ttggccacag gcgagcagga gtggtttgcc gtggtattgg
     2401 gttagaataa aggtattgac gcctgaagaa accactttaa ggggggcgtg ttctattttt
     2461 tttattattt ctttgggagg tttaccttct ttgaagaagc gatttaagcg tttgtcgact
     2521 tgcatgaggt tgagtaattc atggtctgga agatattttg cgagatgaaa ccaaacgtct
     2581 tttggcgtgt ctttaatgag ttgagccatg tgttctggat tgggttggcg attaagttca
     2641 cgaggttctt tcataaaggc ttctttgttg tttaaattgt gatgattgta tcaaaaaata
     2701 ggacaaatgg aattcaaaaa aaacaatcaa ttgatgaacg agcataagag aacttttggg
     2761 tcctcttgct tctaaagttc ttgttttgct taggtgcttc gtttgggtga agggttttct
     2821 ttatcggaat ccggagtgtg gggatgtgct ccggtaaaaa aacgaccgaa tgatgcaatg
     2881 attgcagtga gtatcctgtt gcttttttgg gttttttgct ctccgctgcc atgggcagca
     2941 aagactagag ctttttctgt agctcctttc ctggaagaac cctctctttt tagtgttgca
     3001 ggcaatgtga gaatgggggt gggaacgatg gtcagttcgg ttagaagttc cctgcctgga
     3061 accccaagtt gaccgtaacg attatcccca caagccgcca gtatggggcg atggttttta
     3121 tcacgacctg aaacgaaaag gctacggtgc cttactgtca tggagtctat gcaaaccatc
     3181 tctttgggca gtttgatggg tgtgagtagc attctgtttt ttgtatcccc acagcccagt
     3241 tggccatgat aattccaccc acagtttgct agcatgggat tttggtattt atcacgacct
     3301 aaaattactg tacggttgct gctgatttcg actaactcaa tggaggacat gtcttccggt
     3361 aattggattg gggtgagaat tgttctaatt tttgtaccgt cacaacctag ttgtccatga
     3421 ttgttaagtc cgcaagcagc catcattgga gtgttgtgct tatcacgacc taaaatcact
     3481 gtacggccag ttctgatttg caccgactca atgaaagcaa tgttttctgg taatttgaca
     3541 ggggttagta aagctctatg tttcatgtct ccacaaccca gttggccttt atcattcgat
     3601 ccacaagctg ccagtattgg tttgttgtac tgatcacgac ctaaaagtac cgtatggtta
     3661 tcactgattt gcactgaatc aatagaaact atatcttccg gtagtttgac tatagtgaga
     3721 gttgttctaa tttttgtatc cccgcaaccc agctgaccgt catcattaag cccacaagtt
     3781 gccagtatcg gtttattatg tttatcacga cctaaaacca tcgtacggtt actactgatt
     3841 tgcaccgact cgatggaaga gatatcttca ggtatctggg ctaggataag tgatatttta
     3901 ttttctgtat ccccacaacc gagctgacct ttatcattaa gtccacaagc ggccagtttc
     3961 agttgattgt gtttatcatg gcctaaaatt actgtatggc cataactgat ttgcgccgag
     4021 tcgatggaag agatattttc aggtaatttg actcgggtga ataatgttct gtcttccatg
     4081 tctccacaac caagctcacc aaaatagtta actccacaaa gggctattat cggcatgttg
     4141 tatttatcac gacccaaaat caccgtatga ccataactaa gttgcactga ctggatggac
     4201 aggatatctt caggcaattt gactgtagtg aatgctgttc taattttcat gtctccacaa
     4261 ctcagctgac ctttatcatt taatccacaa gctgccaata ttggtttatt ctgcttatcg
     4321 cgtccccaaa ccactgtatg gtaagggctg atctgtaccg agtcgatgga cgcaaatgat
     4381 tcagggagct tgacaggggt tagtgctgtt ctaattttcg tatccccaca accaagctga
     4441 ccatctttgt tttggccaca ggcgagtagg agtggtttgc cgtggtattg ggttagaatg
     4501 aaagtattga cgcctgaaga aacgacctta agtggggcgt gctctatttt ttttattatt
     4561 tctttgggag gtttaccttc tttgaagaag cgatttaagc gtttgtcgac ttgcatgagg
     4621 ttgagtaatt catggtctgg aagatatttt gcgagatgaa accaaacgtc ttttggcgtg
     4681 tctttaatga gttgagccat gtgttctgga ttgggttggc gattaagttc acgaggttct
     4741 ttcataaagg cttctttgtt gtttaaattg tgatgattgt atcaaacaat aggacaaatg
     4801 gaattcaaaa aaacaatcaa ttgatgaacg agcataagag gactttgggg tcctcttgct
     4861 tctaaagttc ttgttttgct taggcgcttc gtttgggtga agggctttct ttatcggaat
     4921 ccggagtgtg aggatgtgct ccagtaaaaa aacgaccgaa tgatgcaacg attgcagtga
     4981 gtgtcctgtt gcttttttgg ggtttttgct ctccgctgcc atgggcagca aagactagag
     5041 ctctttctgt agctcctttc ctggaagaac cctctctttt tagtgttgca ggcaatgtga
     5101 gaatgggggt gggaacgatg gtcagttcgg ttagaagttc cttgcctgga accccaagtt
     5161 gaccgtaacg attatcccca caagccgcca gtatggggcg atggttttta tcacgacctg
     5221 aaacgaaaag gctacggtgc cttactgtca tggagtctat gcaaaccatc tctttgggta
     5281 gtttgatggg tgtgagtagc attctgtttt ttgtatcccc acagccaagc tggccgtaat
     5341 tattaagtcc gcaagtagct attatgggat tcttgtattt atcacgacct aaaactactg
     5401 tatagtcatc actgatttgc accgactgga tgtaggagat gtttttcggt aatttgactg
     5461 gggtgagtaa tgttttattt tctgtatctc cacaacccag ctggcccgaa tagttggctc
     5521 cacaagcggc gagcatgggt ttgttgcgct tatcatgacc taaaatcacc gtgcgactgc
     5581 tagatatttg catcaattct atggtagtga tgtgttccgg caatttgaca agggttagtg
     5641 atgatttata tgtgtagccc ccacagccca attgaccatg attattaagt ccacaagccg
     5701 ccagtattgg tttgttgtgc tcatcacgac ccaaaactac agtatggtta ccacttattt
     5761 gcaccgactc aatggaaaag attttttccg gtagtttgac tggagttatt gaagttttat
     5821 tttctattcc attgcagcta acatagtcgc aatgattgac ttcgcaactt gctagtattg
     5881 attgtttttg cctatcttgt ccgagaatta gagtgcagga aatattaatt tggactgact
     5941 tgatgtaaac aatatttttt ggcagtttga tgggggtaag ttgagtttta ttttctgtat
     6001 ctccgcagcc cagctgaccg taattattaa gtccacaggc tgctagcatg aggttattgt
     6061 atttgtcatg atataaaatt atcgtatggt aatcactgat ttgcactgac ttaatagaaa
     6121 cgatatcttc tggtagcttc actgtagtga atgctgttct aattgtcgta tccccacaac
     6181 ccagttggcc ttgatcattc tctccacaag ctgccaatat tggtttattg tgtttatcat
     6241 gtccccaaag cactgtatag taacgactga tttgcaccga gtcgatggac gcaaatgatt
     6301 cagggatatt gataggggtg agtgttgttc taattttcgt atccccacaa ccgagctgac
     6361 catctctgtt ttgtccgcag gcgagcagga gtggtttgcc gtggtattgg gttagaatga
     6421 aggtattgac acctgaagaa acgaccttaa gtggggcgtg ttctattttt tttattattt
     6481 ctttgggggg ggtaccttct ttgaagaagc gatttaagcg tttgtcgact tgcatgaggt
     6541 tgagtaattc atggtcagga agatattttg cgagatgaaa ccaaacgtct ttgggtgtgt
     6601 ctttaatgag ttgagccatg tgttctggat tgggttggcg attaagttca cgaggttctt
     6661 tcatgctata tattttccat tagttaaatt atgtgagtaa ttataaaaat aggattaaag
     6721 taacctatct taatcccatc attttcgata aactcggtgt aaagcatcaa taattgtctg
     6781 ttgctcgaga ttcgatctga gattaataaa gtctttcgtt cttatttcgc acaatgtttt
     6841 tacagcagtt aaattttcac caatccatgg aattctgaat gctggggctg atggcgagcg
     6901 tgctccgcct aaacctggac gcccacaata gactgcataa ttaattccta acctggaatc
     6961 aataccaaac caattctcac ttttctcaga tcttccaata tgaaattgca tagatgactt
     7021 tggttgcgag ataaactcta gcggagggtg aaaaacttca tctgaacagt attcgtgaac
     7081 aacatgcata ggcaagagct tttgagcggc cccgactcct tctcgccatt gtttatcaat
     7141 gacatcccag tttttatcgt cctcaggtgt ttctaacaaa tcaagttgta tttgtagttc
     7201 tttaatgagg gtattcttga agtcaaaatg tttttctctg atgggtttgc catgaagttt
     7261 ataagtaact ccatttgtat tgacttcatc gtactgaaaa agcaattctg ctaatatttt
     7321 tttacctttt tcagtcttgg gtatacacgg caacatcttt tcccacatat acttatccaa
     7381 ggcccataag gtatattcaa aagcactgat gttctcaaaa gttcttcctg agcaatccgt
     7441 tactctgcct ttcataaaaa ataaattaat atcctttttt agcattgatt gaactgcatc
     7501 atgctcaccg cgcacagtat ggtgcaagag cttttgaaca tctagaagag gtttaaatag
     7561 cttcaaatgg atttttgagc tttctaccag cttagctaaa tcatttttag gtaatgtgcg
     7621 ggctatttcg atttttagtt cactaagcaa tttgccaaaa aattcaaatt tgtaacgcac
     7681 attttttcct tatgttgaat acacggtaaa tttaaatgtg aattttattt gaaataagtg
     7741 atcatgtaaa taacatgtag atctctattt gttcatgtcc agctgaagaa ctatgtcaat
     7801 gaatacagtt acaacatgca aaaaactgcc ttaactatag aaatatcgga tgtgttgtcg
     7861 taaataattc ataatataaa aagaatatta tttagtcaat tttggcaact a
//
LOCUS       DASLOE010000136         7878 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.137, whole genome shotgun sequence.
ACCESSION   DASLOE010000136 DASLOE010000000
VERSION     DASLOE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.137"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1223
                     /locus_tag="U6309_002290"
     CDS             <1..1223
                     /locus_tag="U6309_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEN5505723.1"
                     /translation="INIDGLKLFINQENNEKPLSSEKNANQYNTTTYPNEQFAIQKLL
                     LSHGQIILNNKGHSTVLKNIQIGAEQFNLKNSPFSVQIKAKLTDAAFLQTAKANINFK
                     GRVSLSPSIIDELNSGISKSSIEGQLQIQNILLNQFAIKKINATLKTHKRDIQFNPLT
                     LSLYNGESIGDMDYVIATQQLLINQTATNLDGKQLITSLLKHPAISGNLDYSIHASIP
                     LKALSIESLVSKGTITLKDGEVYNINLDQLLNNLKVKLNSLMTETPDNIKKLTQSADW
                     DQNKNMQGNTKFKLANFKFQLQTGAISSDSFLLQTDKLQVNGEGRINLLNQEINSNIK
                     ATLNDNSTDNTLQKIQQALGGYFPLVVSGTVENPIVLPDFKAISPVLSSLAIKSALTK
                     PLKIIQKPLKELIH"
     gene            1220..2287
                     /gene="mutY"
                     /locus_tag="U6309_002291"
     CDS             1220..2287
                     /gene="mutY"
                     /locus_tag="U6309_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEN5505724.1"
                     /translation="MSTNSLNQLFSQLLLDWYDLHGRKDLPWQLPRSPYRVWVSEIML
                     QQTQVQTVIPYFNRFIEHFPDIFLLANAEEDEVLSLWSGLGYYSRARNLHNTAKIISD
                     QYNGVFPEDLNILVQLPGIGPSTAAAILSQAFNKPAAILDGNVKRVLSRFFLIEGWPE
                     QAQVKKKLWGLASSCMPNERCADYTQAIMDLGATCCTNKNPQCLRCPVKNHCLAFHNK
                     KQHLYPTKKIKKQRPILSQQFLVLHNGQNQVYLEKRPPSGLWGGLWCLPSINNQTCPI
                     EHIQLFYKLQGDSPKLITRFKHSFSHFHLEITALSIRIEPMNNFISESRGQWFTKETL
                     PTLGLAKPTTLILSKLMETGA"
     gene            2499..3230
                     /gene="ceg18"
                     /locus_tag="U6309_002292"
     CDS             2499..3230
                     /gene="ceg18"
                     /locus_tag="U6309_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg18"
                     /protein_id="HEN5505725.1"
                     /translation="MHLTREVNIMSILSAVSRVINNFSTEIKNRVVEVGSNLKKSTEE
                     FIGEVVIKAAVNRLKQGMLNALSSSNDTTKDNGNDLSTNNPDIEDVSSPTLSTKKKTE
                     ANTTLAGSTGIMQKLLGNIGVNSEQSIEVLSKHFNGAVERFTQDVSKAANEAVCEKIK
                     SIQPEDVIKGINGLIGNTLKVDSESGDTTLSFKLESAVQILGVILAAMGMLMFASQTK
                     STEQKTLEIEHLPTEEIQPPSLAIH"
     gene            3503..4462
                     /locus_tag="U6309_002293"
     CDS             3503..4462
                     /locus_tag="U6309_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEN5505726.1"
                     /translation="MNPILNLIISFIALLWAANHLVAGASGLAIRFQLSPLMIGLTIV
                     ALGTSAPELFISIISSLKDKNDLAIGNAIGSNIANIGLILGIIILIKPTSFHFNKLKK
                     VYPILIIVMLFAYSLILDGYLGTIDGCLFLIGCIAVLCYFIYLASHSPKRDLSVNEFR
                     PAILSYRSTPSNLLSIAIGLLILPICSKYLVYNASEIAKWAGISEFTIGLTIIAIGSS
                     LPELTTSLVAAIKGEDSIAIGTIIGSNIYNLLLILALPGLLNPTKISSVVLWRDMPVM
                     LSITLLLLFLNYYYQKKTSSWPGGILLLVYFCYMASLIINAHS"
     gene            complement(4541..4918)
                     /locus_tag="U6309_002294"
     CDS             complement(4541..4918)
                     /locus_tag="U6309_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEN5505727.1"
                     /translation="MQRIFKLFILIIFIPMMAACIHSTLNLREGIQCFKAQDYRKAFI
                     RLKPEAEKGNPDAQYAVGYMYYYGKGVVEDRKKARFWITCAARAGQPDAIVALGILDR
                     QARRDRELFQDNNLQHFPVDNGL"
     gene            complement(4924..5433)
                     /gene="lspC"
                     /locus_tag="U6309_002295"
     CDS             complement(4924..5433)
                     /gene="lspC"
                     /locus_tag="U6309_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretory system protein LspC"
                     /protein_id="HEN5505728.1"
                     /translation="MKFDIQFLFSANYAKWIIISLISLFGILIIFEWISLFYPLTYVR
                     AIPVSNTKSIAGMREENLNYIFNTSLFGVYVPADLNEDNVKQSMLNVTLVGILFADKI
                     EESQVIIRSASGEEKTYNVGDKIPGGATIKRIMPGGVLVERDGTLESLSLPKNDLTFE
                     PVAKPLKEE"
     gene            complement(5783..7153)
                     /locus_tag="U6309_002296"
     CDS             complement(5783..7153)
                     /locus_tag="U6309_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEN5505729.1"
                     /translation="MSQNQWSLEKIKQYKEDTGQALLSDEQSLVLFGQDFGKLIQSQP
                     VAVSAPQSIESLQSLILFANQYHLPVTIRGNGLSQGGQSLPVPGGLTISMQSFNKPLD
                     LSEDLIWVEANTSWKNLLEKSLLNNKAPYVLPYNCNLSVGGVLSAGGIGASSFKYGVI
                     NAYVSALEVVDGLGRKQIVEKNSPLFQACLSGQGRFGVITKACIQLRSVQPRVKTFFL
                     VYADQNQWFEDIYKIQGKVDYMEIFCSPSIQGARLKEDKRIPMAYWLYGLHLSVEYDR
                     HAENILGQLKPWNVLNIQEESILSYFLRHNSRFDMMKLTGQWDLLHPWYECFVPTSFL
                     TGVLSQLLEELPLHYASLVHVVPIAKQKAGFMMLPDCDSICEFMILNPGVPYSLEESC
                     LKAIENLDKHLLQNNGKRYLSGFLGTELPDNYWLKHFGEKYDSWVGLKKQYDPAGIFS
                     SMLHHI"
     gene            complement(7110..7862)
                     /locus_tag="U6309_002297"
     CDS             complement(7110..7862)
                     /locus_tag="U6309_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505730.1"
                     /translation="MKTVQAYIDSKQHEFMNHPFFNILEELNSIEEISYFVPELTFWA
                     MTFQDILRINEERVTDPYLKKVARHHRLEDAGHEKWFLSDKKYMGEICDNKACDRDDV
                     AWLYSKDSQLVRDAAYSIMSEIYKIEDENLNIVLLLTLESSGHVFFEKVVKQVKKTGE
                     DKNLKYFSSSHLEVELAHALFEEEMERKVYSRRLPISTRREALRMVDRCYDAFSKMFD
                     GLILACNKRLELAKERNQHVAKSVELRENQAV"
BASE COUNT         2612 a         1556 c         1269 g         2441 t
ORIGIN      
        1 aaattaacat tgatggctta aaattattta tcaatcaaga aaataatgaa aagcctctct
       61 cttctgaaaa aaacgctaat caatacaaca ccacaacgta ccccaatgag caatttgcca
      121 ttcaaaaatt attattaagc catggacaaa ttatccttaa caataagggg cattctactg
      181 tcttaaaaaa tattcaaatc ggagcagagc aatttaattt aaaaaactcg cccttttcag
      241 tacagattaa agccaaactg actgatgctg cttttttaca gacagccaaa gctaatatta
      301 attttaaagg tcgagttagt ctatcaccct caattattga tgaactcaat tcaggaatta
      361 gtaagtcttc catagaaggc caactgcaaa tacaaaatat tttattgaat caattcgcta
      421 tcaaaaaaat caatgccact cttaaaactc ataaaagaga tatccaattt aatccactta
      481 ctttgtcact ctataatggg gaatcaatag gagatatgga ctatgtcatc gctacacaac
      541 aattgttaat taatcaaact gcaaccaatt tagatggaaa acaattaatc acttctttat
      601 taaaacatcc tgcaattagc ggtaatttag attactcaat tcatgcctca ataccattga
      661 aagcactcag tattgagagt cttgttagta aaggaaccat tacgcttaaa gatggagaag
      721 tctataatat taatcttgat caattactta ataatctaaa agtaaaactt aattcgctta
      781 tgactgaaac gccagataac ataaaaaaac tgactcaatc tgcggattgg gatcaaaata
      841 aaaacatgca aggcaatacc aaatttaaat tagcaaattt taaattccag ttacaaaccg
      901 gagcaattag cagtgattca tttttgctgc agactgacaa actccaggtg aatggagaag
      961 ggagaattaa tctgctaaat caagaaataa actccaatat taaggccacg ttaaacgaca
     1021 acagtacaga caatactttg caaaaaattc aacaagcatt gggtggctat ttcccactgg
     1081 tagtatctgg cacagttgaa aatcccatag ttttgccaga ttttaaagca attagccctg
     1141 tattaagttc gttagcgatc aaatcggctc tgacaaaacc tttaaaaata atacaaaaac
     1201 cacttaaaga gctaatccat tgagtacaaa ctctttaaat caacttttca gtcaattact
     1261 gcttgattgg tatgacctgc atggacgcaa agatttgcca tggcaattac cccgatctcc
     1321 ctacagagtt tgggtatcag aaatcatgtt acaacaaact caagttcaaa ctgttattcc
     1381 ctactttaat cgttttatag aacatttccc tgatattttt ttgcttgcta atgcagaaga
     1441 ggatgaagtg ctatctctat ggtcggggtt aggctattac agtcgcgcca gaaatcttca
     1501 caacacagca aaaataatta gtgaccagta taatggcgta tttcctgaag atctgaacat
     1561 tttagttcaa ctgccaggaa taggaccatc gacagcagca gcgatacttt cacaagcttt
     1621 taataaacca gctgctattt tagatgggaa tgtgaaacga gttttatctc gatttttttt
     1681 aatcgaaggt tggccagaac aagctcaggt taaaaaaaaa ctatgggggc ttgcgtcttc
     1741 ttgtatgccc aatgaacgat gtgcggatta tacacaagca attatggact taggtgcaac
     1801 atgctgcaca aataaaaatc ctcagtgttt gcgttgtcct gttaaaaatc attgcctggc
     1861 ctttcataat aaaaagcaac atctttatcc aacaaaaaaa ataaagaaac agcgcccaat
     1921 attatcccaa cagttcttgg tgttacataa tggacaaaat caggtttatc tggaaaaaag
     1981 acctccctca gggttatggg gaggcttatg gtgtttaccc agtataaata atcaaacttg
     2041 cccaattgag catatccaac tcttttacaa gttgcaagga gatagcccaa agctaattac
     2101 gcggtttaaa cacagcttca gccattttca tttggaaata acagcattga gcataagaat
     2161 agagcctatg aataatttta tctctgaatc cagaggacaa tggtttacaa aagaaacctt
     2221 gccaacatta ggccttgcca aaccaaccac cttgatacta tccaaattaa tggagactgg
     2281 cgcctagcca agaaccgttt taaaaccacc aaaaggtgta taaactgtta agtccgcata
     2341 agccaatatc aatctaacaa aatctttaca aataatttat ttattcatta tttaataaat
     2401 aatgaacaat tacttttaat ttacattttt ttgtaaagaa atgtttcaat cttaacaatt
     2461 tcttaatgtt cacttaatgt tctcttctta ttatttattt gcacttaaca agagaggtaa
     2521 acattatgtc aatattatct gctgtttcac gtgtcataaa taatttttca actgaaatta
     2581 aaaatagagt tgttgaagtg gggagtaatt taaagaagtc aacagaagag tttattggcg
     2641 aagtagtcat aaaagctgct gttaatcgtt tgaaacaagg aatgttgaat gctttgtctt
     2701 cttccaatga caccaccaaa gataatggta atgatttatc cacaaacaac cctgacattg
     2761 aagatgtatc tagccctact ttaagcacaa agaaaaaaac tgaggcaaat accactctgg
     2821 ctggcagtac cggtataatg cagaaattgt tgggtaatat tggcgtaaat tctgaacaaa
     2881 gtatagaggt attgtccaag cattttaatg gtgctgttga gcgttttacc caggatgttt
     2941 caaaggctgc aaatgaggct gtgtgtgaaa agatcaaaag tatacaacct gaagatgtga
     3001 taaagggtat taacggtcta atcggcaata ctctcaaagt agactcagag tccggcgaca
     3061 ctaccctatc attcaagctt gagagtgccg tacaaatcct gggcgtcatt cttgcagcca
     3121 tgggtatgct gatgtttgca tctcaaacta aatcaaccga gcagaaaact ctagagatag
     3181 aacatctgcc aactgaagaa attcaaccac cttcattggc tattcattaa taaatgctca
     3241 cggcttgttc ttcaaggctg catgccgcaa atcattcttg gtatgtagct tgaagaattg
     3301 gcaacacatt cataaactcc ttaattacaa cgtttgatca ctgaactcgc cccgcttcat
     3361 gatatacaag cattttttaa ttaacatgat gattacatga gctcctggca aaacctgaat
     3421 ataaagtgac acttaatatc tataagttat atcctataaa ccattctcgt attattatca
     3481 ctaaaaaata tcgagtacaa aaatgaaccc tattctgaat ttgattatca gttttattgc
     3541 ccttctttgg gctgccaatc atttggttgc tggtgcctca gggctggcaa ttcgttttca
     3601 attatccccc cttatgattg gtttaactat tgttgcattg ggtaccagtg cccccgaatt
     3661 atttatctca atcatttctt ccttaaaaga taaaaatgac ctggcaattg gaaatgcgat
     3721 tggctcaaat atagcaaata ttggcttaat tttagggatt ataatcctta tcaaaccaac
     3781 ctcttttcat ttcaataaac taaaaaaagt atacccaata ttgataattg tcatgctgtt
     3841 tgcgtacagc ttaatacttg acggttattt gggtacaatt gatggttgcc tttttttgat
     3901 cggatgtata gcagtactct gttatttcat ttatttagct agtcactcgc ctaaaagaga
     3961 tttatcagtt aatgaattta gacccgcgat actatcctat cgttcaacac cctctaattt
     4021 attgagtatc gcaataggtt tattgattct gcctatttgt tcaaaatacc tggtttataa
     4081 tgcatctgaa attgcaaaat gggctggtat aagtgaattc accattggct tgaccattat
     4141 tgctataggt tcctccttgc cagaacttac aacgtccctt gttgccgcta tcaaaggaga
     4201 agactctata gcgataggta cgataattgg ctcaaatatt tataacctgt tgttaatctt
     4261 agctctaccg ggattactaa acccaactaa aattagcagc gtcgtattat ggcgtgatat
     4321 gccagtgatg ctttccatta ccttactttt attgttttta aattactatt atcaaaaaaa
     4381 gacatcctcc tggcctgggg ggatactttt gcttgtgtat ttttgttaca tggcttcttt
     4441 gattattaac gcacacagtt agctaaaact aacctggcct gggttacccg ggtttttaag
     4501 aaagcatttg aatcaaagaa attagcttaa agtaaaaaac ttataaacca ttatcaacgg
     4561 gaaaatgttg caaattatta tcttgaaata gttctctatc ccgccttgct tggcgatcaa
     4621 gaattccaag agcaactatg gcatctggct gacctgctct tgcagcacag gttatccaga
     4681 aacgggcttt ttttctatct tctaccacac ctttaccata ataatacata taacctacag
     4741 cgtactgagc atctggattt cccttttcag cttccggttt taaacgaatg aatgccttcc
     4801 ggtaatcctg tgctttaaag cattggattc cttctcgtaa atttaaggta ctatgaatac
     4861 aggcagccat cataggaata aaaattatta gaatgaataa cttaaagatg cgttgcatgc
     4921 catttattcc tcttttaaag gcttagcgac aggttcaaag gttaaatcat ttttcggaag
     4981 acttaaactt tctaaagtgc catctctttc cacaagcact cctcctggca taatacgttt
     5041 aattgttgca ccaccaggta tcttatctcc tacattatag gttttttctt ccccactggc
     5101 agatcgtata atcacttgag actcttctat cttgtccgca aaaagaatac cgaccaaagt
     5161 aacattcagc attgattgtt ttacattatc ctcatttaag tctgcaggaa cataaactcc
     5221 aaataaggac gtattgaaaa tataattgag attctcttct ctcattccag caatagattt
     5281 tgtatttgaa acagggatag ccctaacata agtcaatgga taaaataagg aaatccattc
     5341 aaaaataatc aaaataccaa acaaggatat taaactaatg attatccatt tagcgtaatt
     5401 ggctgaaaac aaaaattgaa tatcaaattt catacgtttc caatcccagt taaacctatc
     5461 aaaacaaatt catccttctc tttaaataat attgatatat agcatcctac cacaaaatag
     5521 atctttatca ccctcacttc aagatcacta gatcagagct tacaaaatat cccaaggtca
     5581 aggcaaaaaa tgtttttaat gaaggagctt agaataaact aaatgaccga attaaaaact
     5641 ttttttaaca aagagatagg gggtttcgta agccctgtag ataatatatc tttatccagg
     5701 ctgaattcat acctttatgc atcagatcga ccatcatgcc ttgatatctg ttacgaagca
     5761 tgagtttgca caatactatt ttttatatat gatgtaacat tgatgaaaaa atccctgcgg
     5821 gatcatactg ttttttcaaa cctacccatg agtcgtattt ttctccaaaa tgctttagcc
     5881 aatagttgtc tggtaattct gtacctaaga atcctgataa atagcgttta ccattatttt
     5941 gcagcaagtg cttgtcgaga ttctcaatag ccttcaggca actttcctct agggaataag
     6001 gtacccctgg atttaaaatc ataaattcac aaattgaatc gcagtctggt aacatcataa
     6061 acccggcttt ttgcttggca attggaacaa catgaaccaa actggcatag tgaagcggta
     6121 attcctctaa cagttgggaa agtactccag ttaaaaatga ggtaggaaca aaacattcat
     6181 accaaggatg taacaaatcc cattgccctg tcaacttcat catatcaaag cgagagttat
     6241 gccttaaaaa ataagataaa atactctctt cctgtatatt caggacattc catggcttta
     6301 actgccctaa aatattttcc gcatgccgat catattcaac agaaaggtgc aaaccataca
     6361 accaataagc catgggaata cgtttatctt ctttcaatcg agcgccctga attgaaggag
     6421 aacaaaatat ctccatataa tcgactttac cctgtatttt ataaatatct tcaaaccatt
     6481 gattctgatc agcataaacc agaaagaaag ttttgacgcg tggctgaact gatcgtagct
     6541 gaatacatgc cttggtaatg acaccaaatc gaccttgccc tgaaaggcag gcttgaaata
     6601 gaggtgagtt tttttcaacg atttgctttc tccccaaacc atcaaccact tccaatgctg
     6661 aaacataagc attaataaca ccgtatttaa aagaggaagc ccctattcct cctgcagata
     6721 aaacaccccc aaccgacaaa ttacagttat aaggcagcac atagggggct ttattgttca
     6781 gtagtgattt ttcaagcaga ttcttccaac tggtattggc ctcaacccag atgagatctt
     6841 cactcaaatc aagtggcttg ttgaaagact gcatagaaat tgttagtcct ccaggaacag
     6901 gcaatgattg gccgccttga cttaaaccat taccacgaat agttactggt aagtgatatt
     6961 gattggcgaa tagaattaat gattgcaatg actctatgct ttgaggggcg gacacagcta
     7021 ccggctgtga ttgaatgagc ttgccaaaat cctgaccaaa taaaacaagc gattgctcat
     7081 cactgagcaa tgcttgtccg gtatcttcct tatactgctt gattttctct aagctccact
     7141 gattttgcga catgttgatt cctttctttt gccaattcca atcgtttatt gcaagcaaga
     7201 atcaatccat caaacatctt gctgaatgca tcatagcaac gatctaccat ccttagtgct
     7261 tctctacgag tgctaattgg taatcttctg gaatatactt ttctttccat ttcctcttca
     7321 aataaagcat gtgctaattc tacttcaaga tgagaacttg agaagtattt cagattttta
     7381 tcttcacccg tctttttaac ttgtttgaca actttctcaa aaaatacatg tcctgatgat
     7441 tccagggtca acagcaagac aatattcaga ttttcatcct ctattttata aatttcagac
     7501 ataatagaat aggcagcatc acgtaccaat tgggaatcct tactgtatag ccaggcaaca
     7561 tcatcgcgat cacaggcctt gttatcacat atttcaccca tgtatttttt atcacttaaa
     7621 aaccattttt cgtgtcctgc atcttctaat ctgtgatgtc gagctacttt ctttaaataa
     7681 ggatcagtta ctctttcttc attaattcga agtatatctt gaaaagtcat tgcccaaaat
     7741 gttaactccg ggacaaaata actgatttct tctatactat ttaattcttc taaaatgttg
     7801 aagaaaggat gattcatgaa ctcatgttgt tttgaatcaa tataggcttg aacagttttc
     7861 ataacagact ccatataa
//
LOCUS       DASLOE010000137         7835 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.138, whole genome shotgun sequence.
ACCESSION   DASLOE010000137 DASLOE010000000
VERSION     DASLOE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.138"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(109..1437)
                     /locus_tag="U6309_002298"
     CDS             complement(109..1437)
                     /locus_tag="U6309_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEN5505731.1"
                     /translation="MTKSKVLVIDDEPDIRELLTLTLSRMGLTCDAVSDFKQGIEHIK
                     QNYYSLVLTDMRLPDGDGIEIVKFIQKYKPQMPVAVITAYGNVEGAVNTLKAGAFDYV
                     SKPIDLKMLRDLVKTALSMQNPPTEKLDEGLLVGESLVMQQLRENIHKLARSQAPVFI
                     QGASGVGKELVAQLIHANGPRKDKPFIPVNCGAIPGELMESEFFGHKKGSFTGAISDK
                     TGLFVAAHGGTLFLDEIAELPLAMQVKLLRAIQEKAIKPIGELYEVPVDVRILSASHK
                     NLIEEVNAGSFRQDLYYRINVIELRVPTLMERLSDIPCLTEAILRKLSKNQNRNLVKI
                     NPSCFEILSRYHFPGNVRELENILERAMAMCEGEVIEPSDLHLPRTTITPAVAVPSTS
                     TNLNGYLQDQEKELILDALEKTKWNRTAAAQLLGVSFRTLRYRLKKLGLD"
     gene            complement(1434..3020)
                     /locus_tag="U6309_002299"
     CDS             complement(1434..3020)
                     /locus_tag="U6309_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN5505732.1"
                     /translation="MSAARQINNPKMRQWRILLVYNIYRIVSIFLFLGIYYFSYINKY
                     YPLLFLCIAFAYLVFALIFLYFWHRKILSFDKQVFLSGTIDVIAISSMLGIIGNLESG
                     YGILLNVTIAALSILVPGRLAIYFASLASCLLLCGNVLQFFIYNQKDLSTFFYSGIYG
                     AGFFATAITAWYLSNWVRMSESLARHRSYELAGMQRLNEYIVERLHSGIIYVAEGKRI
                     RLMNTAAREFFNLSKHHAIQKLDQLSDSLVSKFDDFLLKTTNNERTAQTIIEDPYLRV
                     HFFSTSVGHKSEVLIILEDMTYIAQQAQQLKLAALGRFSASIAHELRNPLGAIAHAIQ
                     LFGDNEDLNEENTRLKQLIMNNCERMNRVIKNVLQLSRREKSQPQVNELTTFLEHFKQ
                     DFTHNNPCDLTIKLPVNKSLSVVFDKSQLEQILVILCENAIQHGRDHEGNVNIVIAAK
                     CSSNKITLTVCDSGPGIPSEHKDNIFEPFFTTLRGGTGMGLFIARDLCEINQARLNLV
                     KSNKGCCFAITVNPSDELLI"
     gene            complement(3119..4603)
                     /gene="lidL"
                     /locus_tag="U6309_002300"
     CDS             complement(3119..4603)
                     /gene="lidL"
                     /locus_tag="U6309_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LidL"
                     /protein_id="HEN5505733.1"
                     /translation="MQGGMMKQLVVAGFFLLLLTSPLSALDVDTYRSSQKQFNTEDKA
                     EQGDAQAQFELGLKYEKGDGVNKDLKKAIYWYQKAADQGQAEAQNNLGVLYLKGEGVP
                     QNSQQAMYWFKKASEQGLAIGQNNIGILYENGLGVKKDLGQAFIWYQKAAEGGNSDGQ
                     YNLAVMYMYGNGIPKDIKKAIHWYIKAAEQGDLDAQNNLGVLYERGEEVPRDLKAAIS
                     WYTRAANEGSLIAQTNLGVLYMTGDPSIQDGKKAIYWYEKAAAQGGEKAQNNLGYIYE
                     QGIGTEKDMKKAIYWYEKAAENGFTLAQNNLGVLYSNDGELQDYKKAYLWFKKAADQG
                     FAEAQNNLGLMYMKGNGLSVNYHEAVLWYKRAAEQGLPLAQHNLAIMYMKGLGIKKDN
                     KLAIKWYQKAAEKGLDLAQNNLAVMYIRGEGVKRDFKKAMYWYQKAAEQGLDLAQINL
                     GIMYLDGMGVNKDFAKAKYWIGKAKDSGSQDALTIWNKNKLWKY"
     gene            complement(4753..5172)
                     /locus_tag="U6309_002301"
     CDS             complement(4753..5172)
                     /locus_tag="U6309_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1171 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505734.1"
                     /translation="MRFQDSDFEERYNTMWNKIAVSADAQIRQLFGAKGFFSEQQPNY
                     YQLLVNYAQAAKNIVDNLNRQSPMFDDKEYVEGYMIATLQSVYKDFSQYKPRIAGRYG
                     EHSSCVELINKTLDWVQSFDLKLENLSESDDEMKITF"
     gene            complement(5231..6307)
                     /gene="amrS"
                     /locus_tag="U6309_002302"
     CDS             complement(5231..6307)
                     /gene="amrS"
                     /locus_tag="U6309_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEN5505735.1"
                     /translation="MFDTIPTKYWHKLEDGRIQCDVCPRNCKLREGQRGLCFVRANLD
                     NQIKLTTYGRSSGYCIDPIEKKPLNHFLPGSPVLSFGTAGCNLACKFCQNWDISKSRD
                     IDTLADTAMPEDIARAAQNMNCLSVAYTYNDPVIFMEYAIDVAKECHKLGIHSVAVSA
                     GYINPEPCKELYSFMDAANIDLKSFSEDFYYKITGSHLQPVLDTLIYLKKETSVWFEI
                     TNLVIPEENDSDAEFERMSEWIMTNLGPDVPLHFTAFHPDWKMMDKAPTPFSTLQRAR
                     EIAIKTGLHYVYTGNVHDTTGSSTYCPHCKLPVIVRDWYRILDYRVDDTGHCLNCHTQ
                     IAGVFKGAVGHWGPRRVMVKLGCS"
     gene            6379..7722
                     /gene="amrB"
                     /locus_tag="U6309_002303"
     CDS             6379..7722
                     /gene="amrB"
                     /locus_tag="U6309_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEN5505736.1"
                     /translation="MSVRQPAVAGYFYPDDPLTLKQTVLNFLDQAPIHKPAPKAILVP
                     HAGYVYSGSVAASAYASLRDQKNSINKIIILGPAHRLYFKGIAYDPVDKFATPLGEIQ
                     QDKELLTQIIDLPYVYSLPEAHQNEHCLEVQLPFCQMIFSKFKILPLVVGETNPQDVA
                     RLIARVWGGDDTLLIISSDLSHYLPYHIAQREDKKTCLSIDTLNGEEILHDAACGYFP
                     LRGFLYFARQHHIYSRLLDLRNSGDTAGDKERVVGYAAYHFYQKLNFSEHCADELKYI
                     AKETIRLQTEENKALAINYNDYNQLLQIRIPTFITLKKNGLLRGCMGSLIAKERLADN
                     VIYNSIRAAIADPRFPQIRPSELKELSLTISLIKPLSPLYFSSEEELKSQLQIGIDGL
                     VLIYGSYQATFLPSVWESLKTKDEFVNHLKLKMGLSENFWSSEMRALRYSTEIIK"
BASE COUNT         2452 a         1667 c         1272 g         2444 t
ORIGIN      
        1 tacagtaaat gagcacctca gaaacgtttt caggacgaca ataaaacgaa agaggctgac
       61 caggccagag gtctatttta acctttgaaa atcttcaaaa cgcaacatct aatcaaggcc
      121 cagttttttt aatcgataac ggagagttcg gaagctgacc cccagtagtt gtgcagcagc
      181 ggttctgttc cattttgttt tctcaagcgc atcaagaatt aactcttttt cctgatcctg
      241 aaggtaacca tttaagttag ttgacgtgga gggtacggca acagctgggg ttattgttgt
      301 tctgggcaaa tgtaaatcgg atggttctat aacctctcct tcacacatag ccatggcacg
      361 ctcgaggata ttctccagct ctctgacatt tcctggaaaa tgataacgac tcaatatttc
      421 aaaacaggat ggatttattt tcaccaggtt tctgttttga tttttagata attttcttaa
      481 tatcgcctct gtcaggcaag gaatatctga aagcctttcc attaaagttg ggacacgcaa
      541 ttcaatcaca ttaattcgat aatataaatc ttgacgaaac gaccctgcat taacttcctc
      601 aatcaaattc ttatgactcg cacttaggat gcgaacatcc acaggaactt catataactc
      661 acctatcggc ttaattgcct tctcctgaat ggcacgtagc aatttaacct gcatggcgag
      721 aggaagttcg gcgatttcat caagaaacaa agtaccacca tgagctgcaa caaacaaccc
      781 tgttttatca gaaatcgctc ctgtaaagct ccctttttta tggccaaaaa actctgattc
      841 catcaactca ccaggtatag cgccacagtt tacaggaata aatggcttat cctttctggg
      901 accattagca tggatcaact gagcgaccaa ttcttttcca acacctgaag ctccttgaat
      961 aaaaactggt gcctgactgc gagctaattt atgaatgttt tcacgaagtt gttgcattac
     1021 caaactttca ccaaccagta gaccttcatc aagcttctcg gtaggcgggt tttgcatgga
     1081 taatgccgtc ttaacgagat ctctaagcat ttttaagtct ataggttttg atacgtaatc
     1141 aaatgcaccc gcctttaaag tgttgaccgc accctcgaca tttccatagg cagtaatgac
     1201 ggcaactggc atctgcggtt tgtatttctg aatgaatttt acaatttcta taccatctcc
     1261 atcaggtaaa cgcatatcgg ttaaaaccaa agaataataa ttttgtttta tatgttctat
     1321 gccttgctta aagtcagaca ctgcatcgca agttaaaccc atacgtgata aagtaagagt
     1381 taataattct cttatatctg gttcgtcatc tataacaagc actttacttt tggtcatatt
     1441 aaaagttcat ccgatggatt aacagtgatc gcaaaacaac atcctttatt tgattttacc
     1501 aaatttaatc tggcttgatt aatttcacat aaatctctgg caataaataa ccccatccct
     1561 gtaccacctc gcaaggtggt aaaaaatggt tcaaatatat tgtctttgtg ctcagagggt
     1621 ataccaggtc cagaatcaca tacagtcaaa gtgattttat tcgaagaaca tttggcagca
     1681 atgacgatat tcacattacc ctcatgatcc cgcccatgtt gaatagcatt ttcacacaag
     1741 attactaaaa tctgttctaa ttgactttta tcaaaaacaa ctgataacga cttatttaca
     1801 ggtaatttta tcgtaagatc acagggatta ttgtgagtaa aatcctgctt aaaatgttct
     1861 aaaaatgttg taagttcatt aacctgcggt tgagatttct cacgccttga aagctgcaat
     1921 acatttttaa tcaccctgtt cattcgctca caattgttca tgattaattg ttttaaacga
     1981 gtgttctctt catttaaatc ctcattatct ccaaacaatt gaatagcatg agcaattgcc
     2041 cctaagggat tacgtaattc atgagcaata cttgccgaaa aacgccctaa agccgctaat
     2101 ttgagttgtt gtgcttgttg tgcaatataa gtcatgtctt ccagaataat gagtacttca
     2161 gacttgtgac caacagaagt agaaaaaaaa tgcactctta gataaggatc ttcaataata
     2221 gtttgagctg tacgttcatt attcgttgtt tttaaaagaa aatcatcaaa tttggataca
     2281 agtgaatcag atagctggtc caatttttga attgcatgat gtttactcag attaaaaaat
     2341 tcacgagcag cagtattcat caaccgaatt cgcttcccct ctgcaacata aataattcct
     2401 gaatgcaatc gttcgacaat atattcattg agcctttgca ttcctgccaa ctcataacta
     2461 cggtgtctgg ccagactctc agacattctg acccaattgg ataaatacca ggcggtgata
     2521 gccgttgcaa aaaaaccagc cccatagatt ccactataaa aaaaagtgct taagtctttt
     2581 tgattataaa taaaaaattg caaaacattg ccacatagca ataaacaact ggctagtgat
     2641 gcaaaataga tggccaagcg cccgggaact aaaatactta aagcagcaat agtaacattt
     2701 agtaatatgc cataaccgga ttccaaatta ccaatgatgc ctagcatgct ggaaatggct
     2761 atgacatcaa tggtacccga taaaaacacc tgcttatcaa aacttaaaat ttttctatgc
     2821 caaaaataaa gaaaaatcaa agcaaatact aaataggcaa aagcgatgca gagaaataat
     2881 aagggataat atttattgat ataactgaaa taataaatgc ctaaaaatag aaaaatactt
     2941 actattctgt atatattgta aaccaacaat attcgccatt gtctcatttt cggattgttg
     3001 atttggcgag cagcagacat ccttttctaa tccattagat tattcagcgt attttaatta
     3061 tataaccaga cttaagcatt ttgcacaata catcaactaa gacccacacc aatgatcact
     3121 aatatttcca caatttattt ttattccata tggtcagcgc atcttgagaa ccactgtcct
     3181 tggcttttcc aatccagtac tttgctttag cgaaatcttt attaacaccc atgccatcaa
     3241 gatacataat acccagatta atttgtgcca aatccaaacc ttgttcagct gccttttgat
     3301 accaatacat ggctttctta aaatctcttt ttactccttc accacgtata tacataaccg
     3361 ccaaattgtt ttgcgccaaa tccaatcctt tttcagcagc tttttggtac catttaatgg
     3421 ccagtttgtt gtctttcttt atccccagcc ctttcatata cataatcgcc agattatgtt
     3481 gcgctaaagg taatccttgt tctgccgctc tcttgtacca caagactgct tcgtgataat
     3541 taactgacaa tccattgcct ttcatataca ttaaacccaa gttattttgg gcttcggcaa
     3601 atccttgatc tgcggctttt ttgaaccata aataagcctt tttgtaatct tgcagctcgc
     3661 catcatttga atagagaaca cctaaattat tttgagccag agtaaatcca ttctctgcgg
     3721 ctttttcgta ccagtaaata gcctttttca tatccttttc tgtaccaatt ccttgttcgt
     3781 aaatataccc aagattattt tgtgcttttt ccccaccttg tgccgctgct ttttcatacc
     3841 aatatattgc tttcttgcca tcttgaatag aaggatcacc agtcatatac aatacaccaa
     3901 ggttagtttg agcaatcagt gaaccttcat ttgctgctct ggtataccaa ctaatagctg
     3961 cttttaaatc tcttggaacc tcttcgcctc gctcataaag aacaccaaga ttattttgag
     4021 catcaagatc tccttgctct gctgctttga tataccagtg aatcgccttc ttgatatctt
     4081 ttggtatacc attgccatac atgtacatga ctgcaaggtt atattgtccg tctgaatttc
     4141 cgccttctgc agccttttga taccagataa aagcctggcc cagatctttt ttaacaccca
     4201 ggccattttc atatagaatt cctatattat tttgaccaat tgccaaaccc tgttccgaag
     4261 ccttcttaaa ccaatacatt gcttgctgac tgttttgggg cacaccctct cctttaagat
     4321 acaatacacc gagattattt tgtgcctcag cttgtccttg atctgcagct ttctgatacc
     4381 aataaatcgc tttctttaaa tctttattta ctccatcacc cttttcatat ttgagcccta
     4441 attcaaactg tgcttgagca tcaccctgtt ccgccttatc ctctgtattg aattgttttt
     4501 gtgaactacg ataggtatca acatccagcg ctgataaagg ggaagttaga agtaataaga
     4561 aaaatcctgc aacaaccaat tgtttcatca tacctccctg caatcattgt tcaattatag
     4621 cacagttgat gatgaataaa cattgacgct ctgatgccat acaagcaaag tgcatctatt
     4681 aggagtaaga tataatagag aaatgttgct atcccgcatt acagacttct cgtaatacag
     4741 gatatcttgc aattagaaag taattttcat ctcatcatca gattcagaga ggttttctaa
     4801 ttttaaatca aaggattgta cccaatccaa ggtcttgtta atcagttcaa cacagcttga
     4861 gtgctctcca taacgccctg caattctcgg tttatattga gaaaaatctt tatataccga
     4921 ttggagagta gcaatcatat agccttcaac atattctttg tcatcaaaca tgggagattg
     4981 acgatttaaa ttatcgacaa tatttttcgc cgcttgagca tagtttacaa ggagttgata
     5041 gtaattgggc tgctgttcac taaaaaatcc tttggcgcca aataattgcc ttatttgagc
     5101 atcagcagac actgcaattt tattccacat tgtattataa cgttcttcaa agtcagaatc
     5161 ttgaaatctc atgttaattc accatgtaca aaaacatgac atataatact aaaaatttat
     5221 tatgggaaga ttaagagcaa cctaatttaa ccattactct tcttgggccc caatgaccaa
     5281 ctgctccctt aaatactcct gcaatttgcg tatgacaatt taagcaatgt ccagtatcat
     5341 ccacccgata atccaatatt cgataccaat cccttacaat cacaggtaat ttacaatgag
     5401 gacaataagt gctgctgccg gttgtatcgt gaacatttcc cgtatataca taatgtaagc
     5461 ctgtcttgat tgcgatttct ctggctcttt gtaaagttga aaatggagtc ggggctttat
     5521 ccatcatttt ccaatctgga tgaaacgcag tgaagtgtaa aggaacatca ggccccaggt
     5581 tagtcataat ccattcagac atcctctcaa actcggcatc cgaatcattt tcttctggaa
     5641 tcactaaatt agtaatttca aaccatacgc tggtttcctt cttcaaataa atgagcgtat
     5701 ccagaacagg ttgtaaatga gaaccagtaa ttttgtaata aaaatcttca ctaaaggatt
     5761 ttaaatcgat attagcagcg tccatgaatg aataaagctc cttacatggc tcaggattaa
     5821 tataccctgc agagaccgct actgaatgaa tgccaagctt atgacattcc tttgcgacat
     5881 caatggcata ttccataaaa atcaccggat cattgtatgt atatgccaca ctgagacagt
     5941 tcatattttg ggcagccctt gcaatatctt ctggcatggc agtatctgcc aatgtatcaa
     6001 tatctctgga tttgctaata tcccaatttt ggcaaaactt acaggctaga ttacaccccg
     6061 ccgttccgaa agataagaca gggctacccg gcaaaaagtg attcaatggt tttttttcaa
     6121 tgggatctat gcaataaccg ctagagcggc cataggtagt taattttatt tgattatcca
     6181 ggtttgcacg aacaaaacat aaacctcttt gcccttcacg caatttacaa ttacgaggac
     6241 atacatcaca ttgtatgcgc ccatcttcca gcttatgcca gtatttggtt ggaatagtat
     6301 caaacatcaa atagctcaca atcaactctt agtctatatt tataagtata gtatgttcag
     6361 ttgttccgga gtgttgttgt gagcgtccgt caaccagccg tagctgggta tttttatccc
     6421 gacgatccat tgactttaaa acaaaccgta ttgaatttcc tggaccaagc gccaattcat
     6481 aagcctgctc caaaagcaat actagttccg catgctggct atgtctactc aggatctgtc
     6541 gcagcttctg catatgcttc gttgcgagat cagaaaaatt ccatcaataa aattataata
     6601 ttaggccctg ctcacagatt atatttcaaa ggcatagcct atgatcctgt cgataaattc
     6661 gctacaccat tgggagaaat tcaacaggat aaagagttac taactcaaat tatagatctg
     6721 ccttatgttt attcccttcc tgaagctcat caaaatgaac attgtctgga agtacaatta
     6781 cctttctgcc aaatgatatt ttccaaattt aaaatacttc ctctggttgt tggagaaaca
     6841 aatccccagg atgtagcccg tctaatagcg cgagtatggg gtggagatga tactctcctg
     6901 attatcagct ctgatttaag ccattatctg ccctatcaca tagcccaaag agaagataaa
     6961 aaaacttgcc tttccataga cactttaaat ggtgaagaaa tactgcatga tgctgcttgt
     7021 ggttattttc cactgcgcgg tttcctgtat ttcgcacgcc aacaccatat ttatagccgt
     7081 ctgctcgatt tgcgcaactc aggggataca gctggagata aagaaagagt agtggggtat
     7141 gctgcctatc acttctacca aaagcttaat ttttcagaac attgcgcaga tgaattgaaa
     7201 tacatcgcaa aagaaacaat acgattacaa acagaggaaa ataaagcatt ggcaattaat
     7261 tacaatgatt ataatcaatt attacagata cggatcccca cttttattac tttaaaaaaa
     7321 aatggtttgc taagagggtg catgggaagc cttatagcta aagagcgatt agcagataac
     7381 gtcatttaca attccatacg ggcagcaatt gctgacccac gcttcccaca aataagacca
     7441 tctgaactca aggaattgtc tctaactatc tcattaataa aaccattatc tccactatat
     7501 tttagcagtg aagaagaact caaatcacaa ttgcaaattg gcatagatgg cttggtatta
     7561 atttatggtt cctatcaagc tactttttta ccttctgtat gggaatctct taaaacaaaa
     7621 gatgaatttg ttaatcattt aaaattaaaa atggggcttt cagaaaactt ctggtcttca
     7681 gaaatgagag cgcttcgcta ctcaacagaa ataattaaat aacttaataa tgtttgaagt
     7741 aaaaattcag ccaataatac tatttttata aagccctgat tcaaaatact tctagcaatt
     7801 cccaagatcc taatattcac agaacataaa atcag
//
LOCUS       DASLOE010000138         7833 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.139, whole genome shotgun sequence.
ACCESSION   DASLOE010000138 DASLOE010000000
VERSION     DASLOE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.139"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            104..1291
                     /locus_tag="U6309_002304"
     CDS             104..1291
                     /locus_tag="U6309_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEN5505737.1"
                     /translation="MVHFQKMTVQTFLKDYWQKKPLIIRQALPDFTNPLTPDELAGLA
                     LEEEIESRLVYETPDHSPQWNLKRGPFKESDLIGLPKTHWTLLVQGVDRIVPDVYELL
                     EHFNFIPQWRVDDVMISYAALHGSVGPHYDNYDVFLYQAKGRRLWSLTSKKCHTNNFI
                     QGLELRIMKEFEVEEQFILEEGDMLYLPPHIGHYGIAQSEECMTYSFGYRSYQGQELW
                     DSLGDYLSEHGLFKSLYQDPDWSTLKSTSEITPKAWDNARQLLRQVLENDQLMQSWFA
                     CFATSLDQSAEQNLPPPLEEDELLGLDEFIKELANYQEIVRDASCRFAYIMSDQESQC
                     HFYVNGKEWDSRGVSTNLLSLVANNRFLSLKELKPYLSHKTNQLFLYELWKLQWLQIS
                     HDQ"
     gene            1399..1725
                     /locus_tag="U6309_002305"
     CDS             1399..1725
                     /locus_tag="U6309_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505738.1"
                     /translation="MARFSRISLSTVLLSLICFNIFASDTIMITVNTKEKNAIAIGYS
                     VNGNQYGGLGSSYSGHGPKNKEYQFGYKKDSIFGKDISCGSMTLNQDSKVTLVNNNNQ
                     CISVLG"
     gene            1980..2129
                     /locus_tag="U6309_002306"
     CDS             1980..2129
                     /locus_tag="U6309_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505739.1"
                     /translation="MKKIILAVVVISAAVLLSGCGGGCCGASNSCCSYNTGCCGVQNY
                     SGIWY"
     gene            2161..3063
                     /locus_tag="U6309_002307"
     CDS             2161..3063
                     /locus_tag="U6309_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN5505740.1"
                     /translation="MKKNSLLAISEEGFHHIAYTEWGIFEPELPTVICVHGYTRNSRD
                     FDELANYLSSKGRHVFCPDIAGRGDSSWFKNPRYYNFTQYIKDMAVLMARTNAHQIDW
                     IGTSMGGIIGMILAAMPNSPINKLVLNDIGPQIPIHGLRRIAKYAGKDPDFKSLEEAK
                     QHFKASYADFGITNEKQWDVFTKNSVEQRGPNLYVTKMDPAIKRSKSILQIISEFFRH
                     PHKALEGIFYDINLWSIWKQIKCPVLVIHGVNSDILTSEIITQMKKTHALTEVYEVEN
                     AGHAPALLDLTIHQRIEDWLATHQ"
     gene            complement(3093..3995)
                     /locus_tag="U6309_002308"
     CDS             complement(3093..3995)
                     /locus_tag="U6309_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEN5505741.1"
                     /translation="MNSQEQRGSFYAILSGFLYGFIGYFGISAIHGDLSASNMLFWRF
                     LISSIFILIVLIPQLKNINDTYKSMFTAFLSGVLYYGLSTLLYFYASLYIGSGLSMVI
                     FFTYPVIIMVFNYFFYSQTIPKIYYLAISVILIGMALLIDVNEIAFDLWGIFLGLASA
                     FFYACYIVSSKNNEISPNVSTLMVCLGCMTTSLIVACLDQTFKVPTSFPVWLNLFGIG
                     LVATVIPILLMLHSLKYISSEKASILSVLEPVFVVIFGVVLLGEELKPWHALGIVLVL
                     AGALITLFSHRINLNQWLSAIYKS"
     gene            4253..5392
                     /locus_tag="U6309_002309"
     CDS             4253..5392
                     /locus_tag="U6309_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HEN5505742.1"
                     /translation="MFRVLIFLSCLVILTPNTIADTNPCEKHSCIAVIDAGSTGSRLH
                     IYSYDTDDTNTPIHIEEIWNKKIKPGFASIQPNSVTIDAYLTMLLADAPIHNIPVYFY
                     ATAGMRLLPQSQQKKYYDELEYWFRQQSQWQLVEAKTITGNDEALFDWLAVNYKLDTL
                     KSVQNKSVGVMDMGGASVQIVFPMPKNAEISKHNQVELNIYGQNINLYVHSFLGLGQT
                     EMSHQFLNSPSCFANDYPLPDGESGQGNAPSCKEEVTSLMNSVHKVNQQIQPLLALNP
                     VNEWYSIGGISNLASSQLFHFENSELTNQSLLQQGDNQICHQQWDILNGQYPDDEYLY
                     QYCLLSSYYYALMVDGYGINPNQTIHYIPPEQNLDWTIGVVLHRA"
     gene            complement(5397..5930)
                     /locus_tag="U6309_002310"
     CDS             complement(5397..5930)
                     /locus_tag="U6309_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HEN5505743.1"
                     /translation="MHHVEDLISIFNYCFENDYNTRLVRGGDEPIYLPADDQRPYHAL
                     FFAHGFFSSALHECAHWLIAGEERRKLVDFGYWYAPDGRNEEQQTLFQRVEVKPQALE
                     WILSMAAGHKFRVSIDNLNGAEVDTEAFKQSIYKQVFHYCTQGLPQRALHFRNALCAF
                     YRQPSPLRIDDFSIDEL"
     gene            complement(5941..7746)
                     /locus_tag="U6309_002311"
     CDS             complement(5941..7746)
                     /locus_tag="U6309_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1907 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505744.1"
                     /translation="MVRECTSFKGGLNLNFIENAEFESRKNIPPEEQRAVLIRNTLRL
                     LMMGWNTSWTELFSWETFKAIYLQRDPLLLKEFRLAFQKGFDALFTQLSGRQFNSQQN
                     EQIQLYLSNCLSYLPLFDLNPYESIKVPQRINDQWVMVDYYIKPIELTNHRGKNDLIL
                     RDDDRVFAYGLEPIDNPNAQPHLIFMGTTYPAGQGFISQLNTDLKGFETVGYSLYKNG
                     RDRIKDWILKQNSKIHVCGISLGGSLSLLLAIDMGKYLSRVDALNPAGLHDTDNKVKY
                     DKWDKIKKKPLVVVQQQGNDPVSFFGVWKKEWNILHVKPPKDKQGPNLFCDHFLNYAG
                     FAETEFTYVSAEQQNAKRKARNFWLYSLGRGVLYYFGLAPYTYLIRPVIHYIAEKWPL
                     IVFGIIPLLTIGTLIGLSTAGIIPAIVSIGTLAAFAISSGIFYLSFGLSSNEEDKNTW
                     AGMFDFILKGNEKISDENEIKPAKLHHPSTPRNPEMDIYKNETAIDLTYKQINTYYQV
                     TRCLLKQKEFLPLKDKPEKRFGGLSKKEALLASMNPASHDEVISLQIPKAKAVHIKHS
                     LTLAAKIGMENEIELKSALKKEYNQYQIGKHQRFC"
BASE COUNT         2608 a         1516 c         1367 g         2342 t
ORIGIN      
        1 aaattaaact aaatttgtgc tagtcctgtt tgatttacca gttgcattat tgagtctcat
       61 agtgcttaaa tagacaacat ttatcaaact atgattttta ataatggttc actttcaaaa
      121 aatgactgtc caaacctttt taaaagacta ttggcaaaaa aaaccattga tcattcgtca
      181 agccttacct gattttacta atccactcac accggatgag ttggcaggac ttgcccttga
      241 ggaagaaata gaaagtcgtc tggtctatga aacgcccgat cattccccgc agtggaattt
      301 aaaaagaggc ccgtttaagg aatctgattt gataggcttg cctaaaactc actggactct
      361 attagtacaa ggagtagacc gtattgttcc agatgtctat gaattacttg agcatttcaa
      421 ttttatccct caatggcgag ttgatgacgt aatgattagc tatgccgcgc tacatggaag
      481 tgtgggccca cattacgata attatgatgt tttcttgtat caggctaaag gtcggcgact
      541 atggtcatta acctcaaaaa aatgccacac caacaatttt atacaaggtc ttgaactacg
      601 aatcatgaaa gaatttgagg tagaagagca atttatcctg gaagaaggcg acatgctcta
      661 tttacccccc catataggac attatggcat cgctcaatct gaagaatgta tgacttactc
      721 ttttggttat agaagttacc aaggacaaga gttatgggat agtctaggtg attatttgtc
      781 tgaacatggc ttgttcaaat ccctctatca agacccggat tggtccactc ttaaaagcac
      841 atcggaaata acgcccaaag catgggacaa tgcaagacaa ttgctaagac aagtcctgga
      901 aaatgatcag ctcatgcaat cctggttcgc ttgttttgct acaagccttg atcaatcagc
      961 agaacaaaat ttaccccctc ctttagaaga agatgagtta ctcggtttgg acgaatttat
     1021 taaagaattg gctaattatc aggaaatagt tagagatgcc tcatgtcgtt ttgcttatat
     1081 catgtctgac caagaatccc agtgccattt ttatgtcaat gggaaagaat gggacagccg
     1141 tggggtttca acaaacttac tcagtcttgt tgccaataat cgattcctgt ctctaaaaga
     1201 gttaaaacct tatttaagcc ataagactaa tcaactcttc ctctatgaat tatggaaatt
     1261 acaatggtta caaatttctc atgatcaatg atttggttta taaatatctt gaataaaatt
     1321 caccctcaat aggtagacaa aatatagttt tctaattagt ttattagaac attattatat
     1381 ggatatgtag gataaaaaat ggcacgcttt tccagaataa gtttaagtac tgtattgtta
     1441 tctctaattt gtttcaacat ttttgcctct gacactatta tgattacagt taataccaag
     1501 gaaaaaaatg ccattgctat aggttactca gtcaatggaa accaatatgg aggtttgggc
     1561 agctcctatt ctggacatgg acctaaaaat aaggagtatc aatttggata taagaaagat
     1621 tccatttttg gaaaagatat ctcttgtggt tctatgacct taaatcaaga cagcaaggtg
     1681 actttggtaa ataataataa ccaatgcatc agcgttttag gttaattggc tattgcatgg
     1741 aatgtagata ttaattttga aaagacagta gtgcctatcc aacaatattc cgctcaaaat
     1801 aattgaattc aactcgtaga aactcaaatc tgtaattaac agtttataag cccagacctg
     1861 tctcctaaac cagacatttg gcaaccaaaa acaccaaaaa acacaatcaa tacaatcaat
     1921 tggataattt taaattcata tagtatattt aatatacatg gacaaaacaa ggataggcaa
     1981 tgaaaaaaat aatattggcc gtagttgtta tttctgctgc tgtattattg agtggctgtg
     2041 ggggtggatg ctgcggtgcc agcaattcat gttgcagcta taatactgga tgttgtggtg
     2101 tacaaaatta ttccggtatt tggtattgat ttacaattat aaaagccaat agtctgaaat
     2161 atgaaaaaaa acagcttgct tgctatctcg gaagaagggt ttcatcatat tgcttatact
     2221 gaatggggta tctttgaacc tgagttacct acagtcattt gtgtgcatgg gtatactcgc
     2281 aattccaggg atttcgatga attagccaat tatcttagct ccaagggacg tcatgtattc
     2341 tgccctgata ttgcgggtcg aggtgatagt tcctggttta aaaatccccg ctattataat
     2401 tttacccagt atataaaaga tatggcagtc cttatggcaa gaacaaatgc tcatcaaatc
     2461 gattggattg ggacatcaat gggcggcatc attggcatga ttttagccgc aatgcccaat
     2521 tccccaatta ataaacttgt tctgaatgat atagggcctc aaatacctat tcatgggttg
     2581 agaagaattg ctaaatatgc aggtaaagac cctgatttta aaagcctgga ggaagccaag
     2641 caacacttca aagccagcta tgctgatttt ggaattacca atgagaaaca gtgggatgtt
     2701 ttcactaaaa acagtgtaga acaacgaggc cctaatcttt atgtcactaa aatggatccg
     2761 gcaataaaaa gatcaaaatc aattttacaa atcatttcag aattttttcg tcatccccat
     2821 aaagcactgg aaggaatttt ttatgacatt aatctgtggt caatttggaa gcagattaag
     2881 tgtccagtgt tagttattca tggtgtgaat tctgatatct tgacttcgga gataatcaca
     2941 caaatgaaaa aaactcatgc tttaactgaa gtttatgaag tagaaaatgc aggacacgca
     3001 ccagctctat tggatctgac tattcaccaa agaattgaag attggcttgc cactcatcaa
     3061 taaatcacat aagaaatgcc acatctttat tatcaggact tgtatattgc acttagccat
     3121 tgatttaaat taattctatg actaaacaag gtaatcaaag cgcctgccaa aaccaagacg
     3181 atacccaacg catgccaagg cttgagttcc tcaccgagca gcactacgcc aaagatcacc
     3241 acaaaaacag gttctaaaac ggataagata gatgcctttt ccgaactgat gtacttcaag
     3301 ctgtgcagca ttaacaaaat cggtataacc gttgcaacaa ggcctatgcc aaataaattc
     3361 agccaaacag ggaatgatgt tgggacttta aaggtctggt cgaggcaagc tacaatcaaa
     3421 ctggttgtca tacagcctaa acataccatc agggttgaga catttggaga aatttcattg
     3481 tttttacttg aaacaatata acatgcataa aaaaaagctg aggctaagcc cagaaatata
     3541 ccccataaat caaatgcaat ttcatttaca tcaattagca acgccattcc tatcaggata
     3601 acagatatgg ccaaatagta tatctttggt attgtttggc tatagaaaaa ataattgaac
     3661 accataataa tcactggata agtaaaaaat atcaccatcg ataatccaga gcctatatag
     3721 agggaagcat aaaagtacag taatgtcgat aaaccataat agaggacacc acttaaaaaa
     3781 gcggtaaaca tggatttata ggtatcattg atatttttta attgaggaat caaaacaatc
     3841 aaaataaaaa tactggaaat aagaaatcgc caaaacaaca tattgcttgc tgataaatca
     3901 ccatggatag cactaatccc aaaataacca ataaatccgt aaagaaatcc ggataaaata
     3961 gcataaaagg atccacgttg ttcttgggag ttcatgattg agttactcga aataagaggt
     4021 atgatctaac aaataaaaag tattatttta ctacttttat tctattttga aaatcaataa
     4081 tttaaattta aatcaaattg ttgattttca acccataaaa cagcgactct tcatcactcg
     4141 acaaaccaat accccacaat gttttgtaca gtttatttaa acacactcta ttgacaacaa
     4201 aatatacaca atgtaccatt cgaaaacttc aattttggct ctatatcaac taatgtttcg
     4261 agttttgatt tttctttctt gcctagtaat tttgacgcca aacactattg ctgacactaa
     4321 cccctgtgaa aaacactctt gtatagctgt cattgatgcc ggaagtactg gttccaggct
     4381 acatatctac tcctatgata cggatgacac caatactcca attcatattg aggaaatttg
     4441 gaataaaaaa atcaaacctg gattcgcaag catacagcct aattctgtaa caattgatgc
     4501 ctacctgaca atgctattgg ctgatgcacc aattcataat atacctgttt acttttacgc
     4561 aacagctggt atgcgactac tcccccaatc acaacaaaaa aaatactatg atgaattgga
     4621 gtactggttt agacaacaat cgcaatggca gttggtcgag gcaaaaacca tcactgggaa
     4681 cgacgaagca ttgtttgact ggcttgctgt aaattataaa cttgatacat taaaatcagt
     4741 ccaaaataaa tcagttggtg tcatggatat gggcggcgct tccgttcaaa ttgtctttcc
     4801 aatgcctaaa aatgcggaga ttagcaaaca taaccaggta gaactcaata tctatgggca
     4861 gaatatcaat ctgtatgttc acagtttcct ggggttaggc caaacagaaa tgtcacatca
     4921 attccttaac tccccctctt gctttgcaaa cgactatcct ttgccggatg gggagtcagg
     4981 acagggcaat gctccatcat gcaaggaaga agtaacctca ttaatgaata gtgtccataa
     5041 ggtaaatcaa caaatacaac ctttgcttgc tttaaatccc gtgaatgaat ggtacagtat
     5101 tggtggtatt tccaatcttg ccagcagcca attatttcat tttgaaaaca gcgagcttac
     5161 taatcaaagt ttgctgcaac aaggggacaa ccaaatttgc catcagcaat gggatatact
     5221 taatggccag tatcctgacg atgagtacct ctaccaatat tgtcttttat cctcttatta
     5281 ctacgcatta atggttgatg gttatggcat taaccccaat caaaccatcc attatattcc
     5341 gccagaacaa aatctcgatt ggacaattgg agtcgtgctt caccgcgctt gaagcattac
     5401 aattcatcga ttgaaaaatc atcaattctc aggggagaag gttgacggta aaaagcacat
     5461 aaagcattac gaaaatgtag tgcacgctgt ggtaacccct gggtacaata atgaaacacc
     5521 tgcttataaa ttgactgttt aaaggcctcc gtgtctactt cagccccatt taaattatca
     5581 atacttaccc taaatttatg tcccgcggcc atagaaagaa tccactctaa agcctgtggc
     5641 ttcacttcca ccctctgaaa cagagtttgt tgttcctcat tgcgtccatc aggagcatac
     5701 caatagccaa aatcgaccag ttttcttctt tcttctccag caataagcca atgagcgcat
     5761 tcatgcaaag cactgctaaa aaatccatgt gcgaaaaata gcgcatgata aggcctctgg
     5821 tcatctgcag gaagataaat aggctcatct ccccctctaa ccagacgggt attgtagtca
     5881 ttctcaaagc aataattaaa aatactaatt agatcttcta cgtgatgcac gaatcgttac
     5941 ctagcaaaac ctttgatgct tacctatctg atactgatta tactcttttt tcaaagcaga
     6001 ttttaattct atttcatttt ccatacctat tttagctgcc aaagttaaag aatgcttgat
     6061 atgcacagcc tttgccttgg gtatctgaag agaaatgact tcgtcatgcg aggcaggatt
     6121 catgcttgct agtaaggctt cttttttaga tagccctcca aaacgtttct cgggtttatc
     6181 cttaagagga agaaactctt tttgtttcag aagacaacga gttacctgat aataggtatt
     6241 aatttgctta taagttaaat cgatcgcagt ttcgttctta tagatgtcca tttcaggatt
     6301 tctgggtgta gatggatgat gaagtttggc cggttttatc tcattctcat ccgatatctt
     6361 ttcgttacct tttaaaatga aatcaaacat ccctgcccaa gtgtttttgt cttcttcatt
     6421 actacttaag ccaaaggaca aataaaaaat tccactggat attgcaaaag cagcaagagt
     6481 acctattgaa acaatagcgg gaataattcc tgcggtactt aaccctataa gagtaccaat
     6541 agtcagtaaa ggaataatac cgaatacgat taacggccat ttctctgcaa tatagtgtat
     6601 tactggtctg attaagtagg tatagggagc caggccaaaa taataaagaa cccctctccc
     6661 taaagagtaa agccagaaat tccttgcttt ccttttggca ttttgttgct cagcagaaac
     6721 ataagtaaat tctgtttcag caaatcctgc ataattcaga aaatgatcac aaaaaagatt
     6781 ggggccttgt ttatcttttg gaggcttgac atgtaaaatg ttccactctt ttttccaaac
     6841 tccaaaaaaa gataccggat cgttaccttg ctgttggaca acaaccaaag gtttcttttt
     6901 gattttatcc catttatcgt acttcacttt attatcagta tcatgaagtc ctgcagggtt
     6961 taaggcatcc actctggaaa ggtattttcc catatcaata gccaaaagca gacttaaaga
     7021 acctccaagg cttataccac acacatgaat tttactgttt tgttttaaaa tccagtcctt
     7081 aattctatct cttccatttt tatataaaga atagccaacc gtttcaaatc ctttcaaatc
     7141 agtatttaat tgggaaataa agccttgacc tgcaggataa gtggttccca taaaaattaa
     7201 atgaggctgg gcatttggat tatctattgg ctcaagtcca taagcaaaaa ccctatcatc
     7261 atctcgcaaa atcagatcat tttttccacg atgatttgta agttctatgg gcttgatgta
     7321 atagtcaacc ataacccatt gatcattgat tctctgggga actttaatcg actcgtaagg
     7381 atttaaatca aataaaggca aataactaag acagttacta aggtaaagtt gtatttgttc
     7441 attttgttgc gaattaaatt gcctgccact taattgggta aatagtgcat cgaaaccctt
     7501 ctgaaaggct aatcgaaatt ctttcagcaa cagaggatct ctttgaaggt aaatagcctt
     7561 aaaggtttcc caggaaaata attctgtcca tgaagtattc caacccatca ttaacaatcg
     7621 caaagtatta cgaataagaa cggccctttg ctcttcagga ggtatgtttt ttctagattc
     7681 gaactcagca ttctcaataa aatttaaatt caaaccacct ttaaaacttg tacactccct
     7741 aaccataaaa aacctctaaa tagcttgttt ataactatga gttcaagaca attacaatca
     7801 ctggctcgca gactgctaag cgagtcattt aaa
//
LOCUS       DASLOE010000139         7801 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.140, whole genome shotgun sequence.
ACCESSION   DASLOE010000139 DASLOE010000000
VERSION     DASLOE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.140"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            366..1124
                     /locus_tag="U6309_002312"
     CDS             366..1124
                     /locus_tag="U6309_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEN5505745.1"
                     /translation="MKLVFNNDVLAVIDDFLEQPSFEKIWNFIQTEKFKFVHSSKWVN
                     AFSLEDGSPLWSSVTISHPRPEACTTEKVYPTNSTIDLFINELIARSSDYSHLIGFKD
                     RDWDFFYARPYLYPRGSGLSWHTDGKYKISGAYVYYCHPIWDINWGAELLINPTPQLD
                     FDYPEVTLINDKKKKVGYHLNSAELNKYADDGVGYYIVPKPNRLVIFKNNILHCIKKV
                     EKAAGNHVRASITGFFMSNEKIHETKEAEKEHSI"
     gene            1194..2273
                     /locus_tag="U6309_002313"
     CDS             1194..2273
                     /locus_tag="U6309_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505746.1"
                     /translation="MNLVHQITPTLAQEKVNFVISIPHSDEAALSNFKLRVEDIPYNE
                     QPSHYQFKKWSSWCSAHFSSYYYYIKYKNALLRCKQFLYDFGPLSTLDHAALYDHWVW
                     EMKPHVLNQENICWIGYDYSKSKTLSFLSHGTNIELRLLEGNLSDEEFLELCKFFSPL
                     SSSSEILEKSFFELSYWSRYRNCIENACINRNEYKPPSSIWNLRWPMHNIFRGSKPDC
                     YIKYSNQLNLDFIPDSQIIYGNENQIEEIQFVLFPKNGKKNQMAWFRVFNKASFPIKK
                     PALSRLPSMNSFSGYSNFNLTVLSPKSSMIASNIYIASLNNCYGPHDALWWDNENAYL
                     LQLSASANNSIAYLKGILCQLIEDK"
     gene            complement(2293..3126)
                     /locus_tag="U6309_002314"
     CDS             complement(2293..3126)
                     /locus_tag="U6309_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEN5505747.1"
                     /translation="MGSGVFIILSAILHAAWNTFAKRNSTPYYEVLGIMSFATIFSLV
                     FIPFFSGPLFKTSDSFLWALTSGLFEGGYIITLAICLSQYSLGKAYIIMRGSAMIIVW
                     FISACFLGENINLSNIIGVSSVLFGLILTSQTFNPDQSRQLLFPVLCGICIAGYHLCY
                     GRSLAYGANPAALFSAALWIALPCVYLLMNKEQKQVFKEQIKSNWKITSLGGIISALS
                     FLLFLIGLRHSEAGLALTLRNTSVVFAQIFAYFIGEKISQYQWIGAFLVALGASLIYP
                     Y"
     gene            complement(3266..3772)
                     /locus_tag="U6309_002315"
     CDS             complement(3266..3772)
                     /locus_tag="U6309_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN5505748.1"
                     /translation="MDTPSIYVACLASYNSAILHGVWIDATQSEDDIMEEIWEMLDNS
                     SEPNAEEYAIHDYEGFGSIKIHEYESISNIVEYASFIKEHGELGLALLCDYSVDDAQT
                     MLEDHYQGCYDSEVDFSRQLFDDCYAHQMPDNLICYFDFEAFARDLFISDYCSVEFGG
                     QAYIFANY"
     gene            complement(3838..4260)
                     /locus_tag="U6309_002316"
     CDS             complement(3838..4260)
                     /locus_tag="U6309_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN5505749.1"
                     /translation="MTASLNQVQLIGNLGADPKNITGKDGQSFVTATLATNESFKKND
                     EWQTRVEWHQLVLFGKLTKVAEYLKKGSQVYLEGKLRSNQWTDNEGKTHQTLSVVVNH
                     IQLLSHFKSADESSNKTAEHHMVQMREMLQTDSEEIPF"
     gene            complement(4361..4555)
                     /locus_tag="U6309_002317"
     CDS             complement(4361..4555)
                     /locus_tag="U6309_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505750.1"
                     /translation="MQQQEPAQLEIEHLLFELNCCVNDLSRHRLQINPYDIQQAELSL
                     HKLESLISFVKQFDTQRIAC"
     gene            complement(5288..6187)
                     /locus_tag="U6309_002318"
     CDS             complement(5288..6187)
                     /locus_tag="U6309_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HEN5505751.1"
                     /translation="MSKSKLKSALFSINECIKELSVYSHASKADTRHILNRCIKDLHE
                     QGFMVAHIKGLKSKHIHVLVEYWKKQGKNPATIKNYMSKLRKLSIWLNKPEIIKPSND
                     YYQIDNRNYVPTYNKAITNIDFSKCENPMIRLSLEAQYLFGLRREESMKLVISEAWFG
                     ERLILKSSWTKGGIGRAIDITSKEQKEWLEKTIKQISPGHSLIPKNKTYKQYLRQYQT
                     ETEKLGLKNCHGLRHAYAQRRYHDLTRQLSPDKQPFKCPIEGGKTSKYLSQSEKAIDR
                     EAREIISRDLGHSRISITKSYLG"
     gene            complement(6180..6983)
                     /locus_tag="U6309_002319"
     CDS             complement(6180..6983)
                     /locus_tag="U6309_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEN5505752.1"
                     /translation="MRKNSLRQAANRYFQLDNRGSYQEKKKRAYVIQKMIDDLFRIGD
                     VPTSWQNMKSEHIQKLVGYWKRNHVKDATIMRYMTIIRNYLLSINCPIQNIDNKSLQL
                     HRKILKPQRLKWQPNLWESFSEMTSRIIMALQTEFGLTFSEAIQIKPGIHIKEHKLSL
                     TREITFNSVDRTIPFRNEIQKNVVSDLEKLTNRQSLVQFFGYKQVKLSWNHTLMAFDV
                     PVKKSWRYLYAKQMYQYLLPILGNYQTCLILQDELGIKSRNTLWTYLHE"
     gene            7275..7478
                     /locus_tag="U6309_002320"
     CDS             7275..7478
                     /locus_tag="U6309_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505753.1"
                     /translation="MIFPTERPATELGNKVALHKTLDYVSLESRQLFSCASLKNIKVV
                     LAVQGLKENNFVLTPVWRQDKIQ"
     gene            7630..>7801
                     /locus_tag="U6309_002321"
     CDS             7630..>7801
                     /locus_tag="U6309_002321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505754.1"
                     /translation="MKKSQLSRNHIITDPIHGVMSFSKDEKELIKQFIDKPIFQRLKR
                     IKQLGCADMVFPG"
BASE COUNT         2441 a         1350 c         1477 g         2533 t
ORIGIN      
        1 aaaataacat taggtattct ggttctttcc caattaggag cagctttaat cattcgcaga
       61 acaccctaac acgaacccta taattttcta aaaccataag ttcggtttaa atgagaacaa
      121 aatacaatat aaatgatcat tttgtgagtt tatatagatc atagtgtaaa aaaacactcc
      181 ttaatatgat tgtcttgatc aaataataaa attataatcc aatactgtta ttcaaggata
      241 ttaattagga gatgttatga acgtatatta ttctaataga attgataccg acgaagataa
      301 ggatgaatat atgccttggt aaataaagga gatataagta tctcctttta tagttttata
      361 ttgatatgaa attagttttt aacaatgatg ttctagcggt cattgatgat ttcctggagc
      421 aaccatcttt tgaaaaaatt tggaatttta ttcaaactga aaaattcaag tttgttcact
      481 cttctaaatg ggtaaatgct tttagtctcg aggatggatc tcctctatgg agcagcgtta
      541 ctatttctca tcctaggccg gaagcttgca caacagagaa agtttaccct acaaactcaa
      601 ccattgattt atttataaat gaattaattg caagatcctc tgattattct catttaattg
      661 gtttcaagga tagagattgg gattttttct atgcgagacc ttatctatat cctagaggat
      721 caggactttc ttggcatact gatggtaagt ataaaatttc aggagcctat gtatattact
      781 gccatccaat ttgggacata aattggggag ctgaattact aataaatcct actcctcaac
      841 ttgatttcga ttatcctgaa gtgacgttaa tcaatgataa gaagaaaaaa gtagggtacc
      901 atctaaatag cgctgaattg aataaatatg ctgatgatgg agttggatat tatattgtcc
      961 caaaacccaa taggttggtt atttttaaaa ataatattct gcactgcata aaaaaagtag
     1021 aaaaggcagc tggtaatcat gtaagagcat caataacggg tttttttatg agcaatgaga
     1081 aaatacatga aacgaaagag gcggaaaaag aacattcaat ctaacaatta ttttaattag
     1141 tttagcccca ccaaattata gataactaat ctgaatatgc ttggaattat gaaatgaact
     1201 tagtgcatca aatcacacct acactagctc aggaaaaagt aaattttgtt atctcaatac
     1261 cccattctga cgaggcagct ttatctaatt ttaaacttag agtagaggat attccttata
     1321 atgaacaacc aagccattat caatttaaaa aatggtcttc ttggtgttcg gctcatttct
     1381 cttcctatta ctactacatt aagtataaaa atgctctctt aagatgtaag caatttttgt
     1441 atgactttgg acctttatca acattagatc acgctgctct ttacgatcat tgggtttggg
     1501 aaatgaaacc ccacgtattg aatcaggaaa atatttgttg gattggttat gactattcaa
     1561 aatcgaaaac attaagtttt ttatctcatg gaactaatat tgaacttcgt cttcttgaag
     1621 gaaatttgag cgatgaagag ttcttggaac tttgtaaatt tttttctcct ctttcatcat
     1681 caagtgaaat cttagaaaag agtttttttg aactatccta ttggtcacgt taccgcaact
     1741 gcatagaaaa tgcgtgcatt aatagaaatg aatataaacc tccctcaagc atatggaatt
     1801 taagatggcc aatgcataat atctttagag gcagcaagcc ggattgctat atcaagtact
     1861 caaaccaact taacttagac tttattccag atagccaaat tatttatggt aacgaaaatc
     1921 aaatagaaga aattcaattt gtcctgtttc cgaagaatgg aaagaaaaat caaatggcat
     1981 ggtttcgtgt tttcaataaa gcctcttttc caataaagaa gccagccctc tctagattgc
     2041 ctagtatgaa ttcatttagt ggctatagta attttaattt aaccgtctta agtccgaaga
     2101 gtagtatgat tgcgagcaat atatacattg caagtcttaa taattgttat ggaccacatg
     2161 atgccctttg gtgggataat gaaaatgctt atctactgca actaagcgct agcgctaata
     2221 actctatagc gtatttgaag ggaattcttt gtcaacttat agaagacaaa tgatcaatag
     2281 atttatgcac ttttagtatg gatatattaa gctagcgccc aaagccacta ggaaggctcc
     2341 aatccactga tattgggaaa tcttttcacc aataaaataa gcaaatattt gtgcaaatac
     2401 cacggaggta ttacgaagag taagggctag tccagcttca gaatgacgca atccgattaa
     2461 aaatagcaag aaggacaatg cacttataat tcctcctaga gaagttattt tccaattgga
     2521 tttaatttgc tctttgaata cttgtttttg ttctttgttc atcaaaagat aaacacaagg
     2581 taaagcaatc cataaagctg cagaaaagag tgcagctggg tttgccccat atgctaaaga
     2641 acgaccgtag cataaatgat aaccagcaat gcagatgcca cataaaaccg gaaataataa
     2701 ttgccttgat tgatcggggt taaaagtttg gcttgtaagt atcaatccaa aaagaactga
     2761 tgatacacct atgatattag ataaatttat attttcacca aggaaacatg ccgaaataaa
     2821 ccatacaatt atcatggcgc ttcctcgcat aataatgtag gccttcccaa gtgaatactg
     2881 actaagacaa atagctagtg tgattatata acctccttca aataaaccag aggttagtgc
     2941 ccataaaaag gaatcagatg ttttaaataa tggacctgag aaaaaaggaa taaatacaag
     3001 cgagaatata gttgcaaaac tcataattcc caatacttcg taatagggtg ttgaattgcg
     3061 tttggcaaat gtattccatg ctgcatggag gattgcggat aaaattataa atacaccaga
     3121 acccatttca gttcatttcg agataaataa tcgattattg taacattgga ccaacagcct
     3181 ataacataaa atttttaaaa cctaatagaa gttgcaaagt gattttagag tttaaagtat
     3241 taaaaaagat gccccaaaga ggggcttaat agttcgcaaa aatatatgcc tgtccaccaa
     3301 actctaccga acaataatca ctaataaaaa gatcccgagc aaaagcttca aaatcaaaat
     3361 agcagatcaa attatccggc atttgatggg cataacagtc atcaaataac tgcctagaaa
     3421 aatcaacctc tgaatcataa caaccttggt aatgatcctc caacatggtt tgtgcatcat
     3481 ctaccgaata atcgcagaga agagctaagc ctagctctcc gtgttcttta ataaatgaag
     3541 catactccac aatattactt attgactcat actcatggat tttgatgctg ccgaatcctt
     3601 cataatcgtg tatggcatat tcttcagcat tgggttcaga gctgttatcc agcatttccc
     3661 agatttcttc cataatgtca tcttcgcttt gtgtggcatc tatccaaaca ccatgcagga
     3721 tggcactgtt gtatgaggct aaacacgcga cgtagattga aggggtgtcc atgggattat
     3781 ctccttgtat aaagggagca atcccacacg ggagcttgct cccgtggtag ggtatgatta
     3841 aaacggaatt tcttctgaat cggtttgcaa catttcacgc atttgcacca tgtggtgctc
     3901 tgcggtcttg ttgctagatt catcagcaga tttgaagtga ctaagtagct ggatatgatt
     3961 gacgacgacg cttaaggttt gatgtgtctt accttcattg tcagtccatt gattagaacg
     4021 cagtttacct tcaaggtata cttgcgaacc tttctttaaa tattcagcta ctttggtaag
     4081 ttttccaaaa agcaccagtt gatgccattc cactctggtt tgccattcat cattcttctt
     4141 gaatgattca ttagttgcca aagtagcggt gacaaaagat tgtccgtctt tcccagtgat
     4201 gtttttggga tcagcaccaa gattgccaat taattgaacc tggtttaaag atgcggtcat
     4261 tgtattctcc tgttattgtt aaatgtgaac ggcataacag aaaaacatcc gcataggcag
     4321 atgattttct gttatgcgga taagacttaa tttgcttcaa ttaacaagca atccgttgag
     4381 tatcaaactg tttgacaaat gaaatcaggg actccagttt gtgcaaactt aactctgctt
     4441 gctgaatgtc atagggatta atttgcagtc ggtgacgact taaatcattg acacaacaat
     4501 tcagttcaaa tagcaaatgt tcaatttcaa gttgcgctgg ttcctgctgt tgcatggtat
     4561 gctccttagg tctaaagagg attagcccca aagggactat gaatcccaat tgggttatgc
     4621 aaaaaagatc accgaaactt cggttgattt acctggttat taatgatgat gtgctcgaag
     4681 cactcctcgg gcgtaaagcc gtaaagacaa ctcctgatag ggcttccctg ctaaacgctt
     4741 tgccatggaa ttagggaaca agatggcata tcacaagacc tggtggtttc gtcagaattg
     4801 cactgactca tcagttgtgt tgctttcaga ttatcaagcc agtattagaa cgcaagcgtt
     4861 ttttactgtt gatattaaat gaacgtattt gaatcagtca cctaaataga taggcatatg
     4921 aatttgttct ataattgatc aatataatta ttacctgtta ggtgaattat gtctgatcat
     4981 agaaaaacaa ggttagcgtt ttattttctt tgtgagaaag aggcttgctc agagagcttt
     5041 tccttagatg agctggagca ggctgctgag tggagtgcat cgactgtaga tacatatctg
     5101 agtaagaaat ggaagcatat agttagcagg tctgcggatg gtctttatac ctgtgctggt
     5161 atatgtaaaa tgtctctaaa tgagttcgta aatttgcaaa aacaaactgc ttagcttggc
     5221 atgaaaacag tttttttaga gggattgaag ttttttatgg ccaccattta aggaaagcag
     5281 ttccttctta accgaggtaa cttttggtta tagatattct ggagtggccg aggtctctac
     5341 ttataatttc acgagcttct ctgtctatgg ctttttctga ttgagataaa tatttagatg
     5401 tttttccacc ttcaatggga catttgaatg gttgtttatc aggcgataac tgcctggtta
     5461 aatcgtggta tcttctttga gcataagcat gacgcagacc atgacaattt ttcaacccta
     5521 acttttctgt ctcagtttga tattgcctca gatactgttt ataagtttta tttttgggga
     5581 tcaatgaatg gccaggagag atttgtttta ttgttttttc tagccattct ttttgttcct
     5641 tgcttgtaat atctatggct cttccaattc cgcctttagt ccagcttgat tttaatatca
     5701 aacgttcgcc aaaccaagct tcgcttataa ccaatttcat cgactcttca cgacgtaaac
     5761 caaagaggta ttgagcctca agagataagc gtatcattgg attttcgcat tttgagaagt
     5821 ctatattagt gatagcttta ttgtatgtgg gtacataatt tcggttatca atttgataat
     5881 agtcattgct aggtttgatt atctctggtt tattaagcca gatggataat ttcctgagtt
     5941 tagacatgta atttttgata gtggccggat ttttaccctg ttttttccag tactctacta
     6001 acacatggat gtgctttgat tttagtccct tgatatgagc taccataaaa ccttgctcat
     6061 gcagatcctt gatgcaacga ttcaatatat ggcgtgtatc agctttactg gcatgggaat
     6121 atacacttag ttctttgata cattcgttga tagaaaatag tgcgctttta agttttgatt
     6181 tactcatgca ggtatgtcca tagtgtgttg cgagatttta ttccaagttc atcctgtaat
     6241 atcaaacagg tttgatagtt acctaatatt ggcagtaagt attgatacat ttgttttgca
     6301 tatagataac gccaggactt ttttacgggt acgtcaaaag ccatgagggt atgattccag
     6361 ctaagtttga cttgtttata accaaaaaat tgtaccaaac tttgtctatt agttagtttt
     6421 tctaagtcag atacgacgtt tttttgaatt tcatttctga aagggatcgt tctgtccacc
     6481 gagttaaaag tgatttctct cgtgagcgaa agtttatgct ctttgatatg gattccaggt
     6541 ttaatctgta ttgcttcact aaaggtcaaa ccaaactctg tttgtaaggc cataataatg
     6601 cgtgatgtca tttcagagaa cgattcccag agattgggtt gccattttaa tcgttggggt
     6661 tttagtattt ttcgatgcaa ttgcagagac ttgttgtcta tattctgtat tggacaatta
     6721 atgcttagta aataatttct aataattgtc atataacgca taattgttgc atctttaaca
     6781 tggtttcttt tccaatatcc aaccagtttt tgaatatgct ctgatttcat gttttgccat
     6841 gaagtaggaa catctccaat acggaataga tcatcaatca tcttttgtat gacgtatgcg
     6901 cgttttttct tttcttggta gctgcctcgg ttatcaagtt gaaagtaccg gtttgcagct
     6961 tgtcttaagg aattttttct catcatccaa tccgaaaatt catttgccct gtctctggtt
     7021 tagtgttggt cgagcggctc actcaaagat gaaacctggc aaaagccata aagctcgaag
     7081 tggggggcaa aacaatcgtt ataaggcaaa cgatctcgcc gatttacaac agtttttctg
     7141 gttatttcct ccttgtgttg ttgattaata aaaacgacaa aacgatgggc tttgtcacaa
     7201 gtccatggtc aaattgaaat aaattgactt aaactcccga gggagttgcc ctgtcggatg
     7261 acagagattc ctagatgatt ttcccgactg aacgtccagc cacagagtta gggaataaag
     7321 tggctttgca caagacgttg gattacgttt cgctggaatc gcgccagctc ttcagttgtg
     7381 cttcattaaa aaatatcaaa gtggtgcttg ctgtgcaagg gttaaaagag aacaattttg
     7441 tcctgacgcc agtctggcgt caggacaaaa tacagtaatg tgttgatttt acgaagtaaa
     7501 atcaagggtt gtgttctgcg tcactgcaca taaattgcag tgacgcagaa aaaatgcatt
     7561 gtcacagatg atgaatggac atccaatgtg ctatattttt atataattgt cggggctaat
     7621 ggagtagtga tgaagaaatc gcaattatca agaaaccata tcatcacaga cccaattcat
     7681 ggtgttatgt cttttagcaa agacgagaag gaattaatta agcaatttat tgataaaccg
     7741 atttttcaaa gattaaaaag gattaagcaa ctgggttgtg ctgatatggt ttttcccgga
     7801 g
//
LOCUS       DASLOE010000140         7787 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.141, whole genome shotgun sequence.
ACCESSION   DASLOE010000140 DASLOE010000000
VERSION     DASLOE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.141"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            80..1147
                     /gene="dinB"
                     /locus_tag="U6309_002322"
     CDS             80..1147
                     /gene="dinB"
                     /locus_tag="U6309_002322"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEN5505755.1"
                     /translation="MNPIRKIIHIDMDCFYAAIEMRDFPELANKPIAVGGDAKRRGVI
                     ATCNYAARQFGIRSAMPTAHALKLCRELILRPVRMDVYQKESQYIRSLLTEYTDLIEP
                     LSLDEAYLDVTESTQCQGSATWIAEEIRARIYQARQLTASAGIAPNKSLAKIASDWHK
                     PNGQMVIRPEDVSAFVLDLPVRKLFGVGPKMEEKLRALNIKTCADLQRYSIEYLLQKF
                     GTMGQRLYELARGIDNRPVNPERIRKSISVEETYPKDLPNSEACLAVLPDLMARLEAR
                     IQRAGKISGIHNLFVKLKFNDFQQTTIERVMDKLDLIVLRQLIQEGFARRGMPVRLLG
                     IGIKLKQENTYQSVQLPLLDL"
     gene            1391..2272
                     /locus_tag="U6309_002323"
     CDS             1391..2272
                     /locus_tag="U6309_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEN5505756.1"
                     /translation="MQPFIIDEKLVCRLIASQFPHYSNLPVKPVAVGGWDNRTFHLGK
                     DMLVRLPSAEQYELQVEKEQQWLPQLASLLPVPIPTPLAKGMPGEGYPWKWSIYRWLE
                     GETIVSANLSNLNEIAKDLATFLTAFHQIDSSGGPKPGMHSFYRGGDLKIYDPETRQA
                     IDYLKGKIDTDHATEIWETALNTSWQGTPVWVHGDISAGNLLVKNGKLCAVIDFGQLS
                     VGDPACDLAITWTLFAGDSRKIFREMLALDKGTWFRGQAWALWKALIVAAGIVNTNAM
                     EVQKSCHIINELFLTEL"
     gene            2414..2734
                     /gene="sugE"
                     /locus_tag="U6309_002324"
     CDS             2414..2734
                     /gene="sugE"
                     /locus_tag="U6309_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEN5505757.1"
                     /translation="MLLPWLYLIIAGLLEICWAVGLKYTDGFSKWQPSLFTATTLIIS
                     MFLLAKAAQTLPIGTAYAVWVGIGSLGTAIMGILLFQEHLSVFRLLYLVLMLISIFGL
                     KYTA"
     gene            2803..3534
                     /locus_tag="U6309_002325"
     CDS             2803..3534
                     /locus_tag="U6309_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEN5505758.1"
                     /translation="MKISRLKTFWIITRLFYYTGVACTRSIFKYLFSTPTRPWVDKVI
                     HHWVDQLLNAVKVEYKVINPHNVQPQPGKATILMCNHSSAYDIPLGFKAFPNNSIRML
                     AKRELSKIPLLGKGMAAAEFPFIDRKNRFQAIKDLEFAQQLMESGIILWIAPEGTRSK
                     DGKLAPFKKGGFITAIQAKATIIPIGIRGAFDILPARTFNIHLNQKAEIHIGEPIDAA
                     QYSIENKEELIEKTHQSIKKLVGEN"
     gene            3745..5961
                     /locus_tag="U6309_002326"
     CDS             3745..5961
                     /locus_tag="U6309_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN5505759.1"
                     /translation="MSINKIEEDRDISSDLEMKAKMYLVHRMDKAGPLTVKDVRILIN
                     KLSKDLQKNQLTLIDCQLIINLVESRFTLVKQATRYLQFFLNKLDGKSINPILIPLSN
                     KPFWQNEPHPFDHYRSTDQLPEKVDIVIIGAGLTGASTAYHLIDAVKKRHLTVVLLDK
                     GNPATEASGRNGGNFELLPENSVGIYSGLVSERFRFLKRCYPHVHPSILQIESEYHAS
                     LVFQFALKNRNRFKEIARKERIYCDLAAKGWLYIAHTEEEEQAICDEVTLAAQHGERI
                     YIWSRKKIREQFGINSKFIGRFIPDDGTYNPFKYTCCLIKAAIKSGIKLYTFVKVQQI
                     KSIDTEYHQLKTNMGLLKARQVIVATNAFTSELLPELRAIKPHQSQIAITDSTPDYCK
                     GRLITSEDGPLYLNQPRVRSKNGLVPLLIGAGDDRPMKNPYYRKRSKKVHDLIVQQRD
                     KYFPNLKGRPFSTEWVGALAFTPDQLPAIGYLSPGIIIVMCCNGYGGSYTTAAGLAAA
                     EIALTGKNPPWLPADVFSPKRLLSDKPLFWEDSDSLWRIGKTLCTTLNNLNQLLAESL
                     SYQSMYQPYSTISLLPHGHQEKNCNLDPLRVMHQLAIFRTFAYQELEMLIRYTSPVCL
                     SKGEILFKQGDAGHSCFILIQGKINLYYEHAEGFTSMVAELEAGSVFGQMALFLENKR
                     MATCQATENCSMLEIMVEPCKSLFIQQQALGIKLLRLLNQGVIDALHKLNKRLKYT"
     gene            complement(6059..7783)
                     /gene="ggt"
                     /locus_tag="U6309_002327"
     CDS             complement(6059..7783)
                     /gene="ggt"
                     /locus_tag="U6309_002327"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEN5505760.1"
                     /translation="MKDHYKWLSCLFFFICNIYQVNAKTFPQHPPGYAVASAHPLATH
                     AGLEILAAGGNAFDAAVAVAAALAVVEPYHSGLGGGGFWLLHQEKEHLNLFIDGREVA
                     PLAASKDMYLAPDGQVIPGLSLNGGLSAAIPGQPAALVYIAKNYGRLPLSKTLAPAIK
                     LAEEGFPVDPQFRYFSTLDDRLENLKKYPDTAAIFLKNGQPFEIGEKLVQKDLAKTLR
                     LIAKYGNAGFYSGETASLLVQGVNAAGGTWSLDDLAQYKIKVRPPLISAYHNMLIITA
                     PLPSAGGVALVTMLNILSKFPLSSFPKLKWVHYVVESMRLAYWQRDQFLGDPDFIKVP
                     LDKLISSDNAKQLSRLISPNKAIDSKVLQGSVPTGKHSNTSHISIIDTEGNRVAATMT
                     INYIFGSSVVAKGTGVLLNDEMDDFSTKVGEQNVFGLVGSERNAIEPRKRPLSSMAPT
                     FLEMPGRVAILGTPGGSRIPTMVLLASLLFYDGYGAISMVSAMRFHHQYLPDVLQFEP
                     DTFPSSIQQGLQAMGYHLMPLKQYYGDMQAITWDKESNVLTAASDPRHIGLAAAISNM
                     AYGYGVNY"
BASE COUNT         2465 a         1612 c         1501 g         2209 t
ORIGIN      
        1 tagactaaac tttacagctt caagaaagtc ggctaaggcc taatatgaaa tctctgttat
       61 tgcaatctgt gattgaaaaa tgaatccgat tagaaaaatt atccatattg atatggactg
      121 tttttatgcg gcaattgaaa tgcgtgattt ccctgagctg gcaaataaac ctatcgctgt
      181 aggcggggat gcaaaacgtc gtggagttat agcgacctgt aactatgctg cccgccaatt
      241 tggcatacgc tctgcaatgc caacagccca tgcgcttaaa ctttgccgag agttaatctt
      301 gcggccagta cgtatggatg tataccaaaa ggaaagccaa tatattagat ccctattaac
      361 agaatatacg gacctcattg aacctttatc attagatgaa gcctatctgg atgtcactga
      421 atccacacaa tgccagggaa gcgccacctg gattgctgaa gaaatacgcg cccgaattta
      481 tcaagcaaga caactcactg cgagtgcggg cattgcgcca aataagagtc tcgcaaaaat
      541 agccagtgat tggcataaac cgaatgggca aatggttatt agacctgagg acgttagcgc
      601 atttgttctt gatttaccag tacgcaagtt atttggcgta gggcccaaga tggaggaaaa
      661 attgagggcg ctcaatatta aaacctgtgc tgatttgcag cgatattcca ttgaatattt
      721 attacaaaaa tttggcacca tgggtcaaag actttatgaa ttagcgcgag gcattgataa
      781 tcgccccgtc aatcctgagc gtattcgaaa atccatcagc gttgaagaaa cctatccaaa
      841 agacttgcca aatagtgaag cttgcctggc agtcttgcca gatcttatgg cgcgccttga
      901 agcaagaata caacgtgcag gtaaaatttc tggcattcac aatttatttg taaaattgaa
      961 gtttaatgac tttcagcaaa caactattga acgagttatg gacaaacttg atttaatcgt
     1021 tttacgccaa ttgatacaag aaggttttgc cagaagagga atgccagtac gattattagg
     1081 tataggaatt aaattgaaac aagaaaatac gtatcaaagc gtacaattgc cattacttga
     1141 cttataacaa gtatctaaac agttgatatc agattctttc ccaaacttgc cataattatc
     1201 agaacttaat acatcaactc tcttttttat gcgacaccct gcaatagtta acaaaatcag
     1261 gacttttata ttgaaaatat caatcaacgg ctgcacgcaa tctagttttc ataacataaa
     1321 caaatagata ggataaattt aaaaataatc ttattattcc tgcatcataa cacttggaaa
     1381 tattattttg atgcagccat tcattatcga cgaaaaactg gtttgtcgtt taattgcgag
     1441 ccagttcccc cattattcca atctccctgt gaagccggta gctgtcggtg gatgggataa
     1501 ccggacgttt catcttggca aagatatgct tgtacgactg cccagtgccg agcaatatga
     1561 attgcaggtt gaaaaagagc aacaatggct cccacaattg gcttccttgc tacctgttcc
     1621 aattccgaca cctttggcaa agggaatgcc tggcgaagga tacccatgga aatggtcgat
     1681 ttaccgttgg cttgaaggag aaacgattgt ctctgccaat ttatctaacc tgaatgaaat
     1741 cgcaaaagat ctggcaacgt tcctcactgc ttttcatcaa attgattctt ccggtggccc
     1801 caaacctggg atgcacagct tttatcgcgg tggagatttg aaaatttatg atccagaaac
     1861 ccgtcaagct atcgattatt taaaaggaaa aattgataca gaccatgcca ctgaaatttg
     1921 ggaaaccgct ctgaatacct cttggcaagg cacacctgtg tgggttcatg gtgatatcag
     1981 cgcgggtaat cttctggtta aaaatgggaa attatgcgct gttatcgatt ttgggcagtt
     2041 gtccgttggc gatccggcgt gtgatcttgc tattacctgg acattatttg caggagacag
     2101 cagaaaaatc ttccgtgaaa tgcttgcttt agacaaaggc acttggttcc gtgggcaagc
     2161 atgggcttta tggaaagcgt taatcgtagc agcaggcatt gtaaacacca atgcaatgga
     2221 agtgcaaaaa tcctgccata ttattaatga gctatttttg actgagctat aatctatacc
     2281 atatcgctca atttgactca gggaatacta tttccttgta agcagtgata tgctataaaa
     2341 taatctccgg acgagacgcg ccgtacccgg ttaagacaag acgacgcctt taatcaatct
     2401 aaaaggagtc gttatgctac taccctggct ttatttaatt atcgcggggc tcttggaaat
     2461 ctgttgggct gtcggtttaa aatataccga tggtttttcg aaatggcagc caagcctgtt
     2521 tacagccacc acactgatta ttagtatgtt tttattagcc aaggctgccc aaacattacc
     2581 tatcggcact gcctatgcgg tatgggtagg gataggttca ctggggactg caatcatggg
     2641 aatactgctg tttcaggaac atctatctgt cttccgattg ctataccttg tattaatgct
     2701 gatatctatc tttggtttaa aatacacggc ttgatttgaa tcgttccagt attcctcaac
     2761 ttgttgtatg atggccctct cataatccca tgcgctctaa cgatgaaaat aagtcgatta
     2821 aaaacttttt ggataattac ccgcctattt tattacacag gtgttgcttg cactcgctcc
     2881 atatttaagt atctttttag cacccctacg aggccatggg ttgataaagt catccaccat
     2941 tgggttgatc aactcttaaa tgctgtgaaa gtagaatata aagtaatcaa tccacataat
     3001 gtccagcctc agcctggcaa ggcaacgatc cttatgtgca atcattccag tgcttatgat
     3061 attcctttag gcttcaaagc attccctaat aactctattc gcatgcttgc caaacgagaa
     3121 ctctccaaaa ttccactttt agggaaggga atggctgcag cagaattccc ttttattgac
     3181 agaaaaaaca gatttcaagc cataaaggat ttggaatttg cccagcaact gatggagagt
     3241 ggcattattc tatggattgc accagaagga actcgctcaa aagatggcaa actagcccca
     3301 tttaaaaaag gtggttttat cactgctatt caagccaagg ctactataat tcccatagga
     3361 attcgcggag cttttgatat tctccctgcc cgcaccttta atatacattt gaatcaaaaa
     3421 gcagaaattc atattggcga gcctattgat gcagctcaat attcaattga aaataaagag
     3481 gaattaatcg aaaaaaccca tcaatcaata aaaaagctgg ttggggagaa ttgaaaaaac
     3541 tcacatcccg gtttgctgaa aaagatgact aaaaatacct acaataacta ataaaaaaaa
     3601 gaccacgtta tacaaaaaat aaaattgatt cctacaatag aataaacaat ccaggctacc
     3661 cgtcttttaa tatcggtact aactgttttg aaatttccta aaacttcacc tagactttaa
     3721 aaagaaaatc aatatgaccg tgccatgagc attaataaaa tagaagaaga cagggatatc
     3781 tcttctgatc tggaaatgaa agccaaaatg tacttggtac accgaatgga taaagccggg
     3841 ccattaacag ttaaagatgt tcgcatcctg ataaacaagt taagtaaaga tttgcagaaa
     3901 aatcaattaa cattaattga ttgtcaacta ataataaatc ttgttgaatc tcgctttact
     3961 ttggtcaaac aagcaacaag atatctacag ttttttctaa ataaacttga tggcaaatca
     4021 attaacccaa tcttaatccc actttcaaat aaaccctttt ggcaaaatga accacatcca
     4081 ttcgatcatt atcgatctac cgatcaacta cctgaaaaag tagatatcgt aatcatagga
     4141 gcaggtctaa ctggcgcttc taccgcatat catctgatcg atgcagtcaa gaaaagacac
     4201 cttactgttg tattgttaga taagggaaat ccagccactg aagccagtgg tcgcaatgga
     4261 ggtaattttg aattattacc tgaaaactct gtgggaatct atagtggttt agtctcagag
     4321 cggtttcgtt ttttaaaacg atgctacccc catgttcatc cgtcaatatt acaaattgaa
     4381 agcgaatacc atgcctcact ggtttttcaa tttgctttaa aaaatcgcaa tcgctttaag
     4441 gaaattgcca gaaaagaacg tatttattgt gatctggcag caaaagggtg gctctacatt
     4501 gctcatactg aagaggaaga acaagcaatt tgtgatgaag tcactctggc tgcccaacat
     4561 ggcgagcgca tctacatttg gtctcgaaaa aaaattagag aacaattcgg catcaactcc
     4621 aagttcatag gtcgatttat tccagatgac ggcacctaca atccctttaa atatacgtgc
     4681 tgtctgatta aggcggcaat caaaagtggt atcaagctct atacgtttgt gaaagttcaa
     4741 caaattaaga gcatagatac agaataccat caattgaaaa ccaatatggg tttgcttaag
     4801 gctaggcaag tgattgttgc aacaaacgca tttactagcg agttgctccc ggaattgaga
     4861 gcgattaagc ctcaccaaag tcaaattgca attaccgatt caacaccaga ttactgtaag
     4921 ggtcgattaa taactagtga agatggaccg ctctatctca atcaacccag agtcagatca
     4981 aagaatgggt tggtgccatt gcttattggt gccggtgacg accgaccaat gaaaaaccct
     5041 tattatcgta agcgttcaaa aaaagttcat gatcttattg tgcagcaaag agataagtat
     5101 ttcccgaacc ttaaagggag accattttca accgaatggg ttggcgcctt agcttttaca
     5161 cctgatcaat tacccgctat tggttacctg tctccaggaa taattattgt gatgtgctgt
     5221 aatggatatg gaggaagcta taccacagca gcaggacttg ccgcagctga aattgcatta
     5281 actggaaaaa atcccccatg gttacccgca gacgtatttt ctcctaaacg cctcctgtca
     5341 gacaagccac tattttggga agatagtgac agtttatggc ggattggaaa aacactttgt
     5401 acaacactta ataatctcaa tcaacttctg gctgagtccc tttcatatca gtccatgtat
     5461 caaccttatt ctacaatttc tttattaccg catgggcacc aagagaagaa ttgcaatctt
     5521 gacccactaa gagtaatgca tcaattagca atctttagaa cttttgccta tcaggaatta
     5581 gaaatgttaa ttcgctatac ctcgcctgtt tgcttaagta agggagaaat tctttttaaa
     5641 caaggtgatg cgggacattc ttgttttatt ctgattcaag gaaaaatcaa tctttattat
     5701 gagcatgctg aaggctttac ttcaatggtt gcagaactgg aggccgggtc agtatttggt
     5761 caaatggcat tatttcttga gaataaacgt atggccactt gccaagctac agaaaactgt
     5821 tccatgttag aaatcatggt agaaccatgt aaatcgcttt ttattcagca acaggctctt
     5881 ggaattaaat tactccgcct cttaaaccag ggagtcatcg atgcgttaca caaactcaat
     5941 aagcgattga aatacactta acaaaaatca taaaacaaat attgattttg attgctttat
     6001 tcagcactaa caatttacgc gccaagtgta gttggccaat tcattttaaa aatgtattct
     6061 agtaattaac cccataccca tatgccatat tagatatcgc agcagctaaa ccaatatgtc
     6121 ttggatcaga agcagccgtt aagacattac tttctttgtc ccatgtaatg gcttgcatat
     6181 ctccataata ttgtttcaat ggcattaaat ggtatcccat agcttgtaaa ccctgttgaa
     6241 ttgaacttgg gaaagtatca ggctcaaatt gcaaaacatc tggtaaatat tgatgatgaa
     6301 atcgcatggc tgaaaccata ctgatagctc catacccatc ataaaataat agtgaggcaa
     6361 gtaaaaccat agtaggaata cggcttcccc caggagtccc caggatagct acacgccctg
     6421 gcatctccag gaaggttgga gccatgctgg atagcggcct ttttcttggt tcaatggcat
     6481 tgcgctcact tcccactaaa ccaaagacat tttgctcacc cactttagta gaaaaatcgt
     6541 ccatttcatc atttaataat acccctgttc ccttcgcaac tacactggag ccaaaaatgt
     6601 aattaattgt cattgttgca gcaacacgat ttccttccgt atcaataatc gaaatatgtg
     6661 aggtatttga gtgttttccc gttggaactg agccttgcaa gactttacta tcaatcgctt
     6721 tatttggtga aatcaagcgg cttaattgtt tcgcattatc tgaggaaatc aatttatcaa
     6781 gtggaacctt aataaaatca ggatcaccca gaaattgatc tctttgccaa taagccaatc
     6841 tcatcgattc aacgacgtaa tgaacccatt ttaatttggg aaatgaagac aaaggaaatt
     6901 tggataatat attgagcatg gttaccaatg ccactccacc ggccgaaggc agaggagctg
     6961 tgataattaa catattatga taagcactaa ttaaaggtgg cctcacttta attttatatt
     7021 gagccagatc atcaagagac caagttccac ctgccgcatt gacaccttga actagcaaag
     7081 aagccgtttc tcctgaataa aatccagcat ttccgtactt ggctatcaac ctgagtgttt
     7141 ttgccaaatc tttttgaaca agtttttctc caatttcaaa aggctgacca ttctttaaaa
     7201 aaatagcggc ggtatcggga tatttcttca agttttccaa tctatcatcc agagtggaga
     7261 aataacgaaa ttgaggatct acaggaaaac cctcttccgc aagcttaata gccggagcta
     7321 atgtcttgct aagcggaaga cgtccatagt tcttggctat ataaaccagg gcagctggct
     7381 gtccaggtat ggccgcagat aaaccaccat ttagagaaag gccggggatc acttggccgt
     7441 caggagccaa atacatatcc ttacttgcgg ccagaggagc tacctccctg ccatcaataa
     7501 agaggtttaa atgttccttt tcctgatgaa gtagccaaaa acctcctcct ccaagtcccg
     7561 aatgatatgg ctcaactact gccaaggcag cagctaccgc aactgcagca tcaaaagcat
     7621 ttcctcctgc tgccaaaatc tctaaaccgg catgagttgc caaggggtgg gcacttgcta
     7681 cagcatatcc aggcgggtgt tgcggaaatg tttttgcatt aacctgataa atattacaaa
     7741 taaaaaaaaa taaacaactc agccatttat aatgatcttt cattttg
//
LOCUS       DASLOE010000141         7780 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.142, whole genome shotgun sequence.
ACCESSION   DASLOE010000141 DASLOE010000000
VERSION     DASLOE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.142"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            153..1442
                     /locus_tag="U6309_002328"
     CDS             153..1442
                     /locus_tag="U6309_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN5505761.1"
                     /translation="MSQLKKIVKHLDESMQRSLSNSAHQLVAVGAIAFVGFPLFYVIW
                     AFWLPQPYENLPLRLIGSLLGLGLMLTPYWPLKWKQYLSWYWFLTLLFTLPYFFTFLF
                     LMNQASVISAMSLLCGVFLLVLLVDLLSLSIVLILGFSLALVSYYLVSPQMYFGEEHI
                     QMTLVIIFTIIAGSTLNYKTAMLQQQKLAGMAAAAGMIAHELRSPLLGIKSGAQALVQ
                     YLPRLIDGYQLAKKQELISSPLRDRLLLQLGGVSNRIVSEIDYANTIIDMLLVKASRD
                     NILQNCILETCSIAECLSEAITRYPFQSPRERTLITWTGDFQFKGSKLLMQHILFNLI
                     KNALYVIAMAQRGEINIWTESGDKYHYLYFKDTAKGMSKQQLNRLFNHFYTTTFMGTG
                     IGLSFCKLVMNAFGGDIVCESKEGEYALFILSFPGSV"
     gene            complement(1530..2093)
                     /locus_tag="U6309_002329"
     CDS             complement(1530..2093)
                     /locus_tag="U6309_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEN5505762.1"
                     /translation="MANSQEFYQPPVDELKRNWGWILGFGIFFLILGCVGLGMVVGLT
                     LVSMFFFGALLIVGGISHIIDVFKYKEWKGMIWQALIAVLYIAAGCIVLYDPFLASTL
                     ITAFLAAVLIVIGLTRMIMAFALKDSKGWGWLLLAGITAIILGVLILMQWPVSGLWII
                     GLFIAIELIVTGWTYIFIAISVKTAKL"
     gene            2374..3399
                     /locus_tag="U6309_002330"
     CDS             2374..3399
                     /locus_tag="U6309_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN5505763.1"
                     /translation="MQHFSIPTCYFPSTAVFVDDSRDFLLNFVLQLDEGLAYRVFDSP
                     FEALDCIKQKRCELELLSERCLSEYTEAKNCPLTNHTINLDLAAIHAEVYNSRRFSEI
                     SVVVVDYAMPGMDGLEFCRRIEDTNIKKILLTGQADEKLAIAAFNEGLIHRYIKKSDP
                     DVASLITQSIHDLQLQYFQSMSDMIVRMLSVTSPNCLHDKKYAELFWRLCREKGIVEF
                     YLADNSGSFLMLDDDANISFLIVKNEADMQLHYDLALDNGASGDVLDQLHNGEKIPCF
                     WQSNLVTPQWNEWSNCLVPANRFVSDETYFYAYVQGAVLFDVRLDKILSYHQYLEELD
                     AEEMFLN"
     gene            complement(3481..4731)
                     /gene="cqsA"
                     /locus_tag="U6309_002331"
     CDS             complement(3481..4731)
                     /gene="cqsA"
                     /locus_tag="U6309_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing autoinducer CAI-1 synthase"
                     /protein_id="HEN5505764.1"
                     /translation="MSNTEYQTIDNETQYINGKEKHQNHAYPEYINHSMEKYYEGRVN
                     KSWQGKHILKGKTPTANSLIFSSNDYLNISQHPQLIKAQIAAMQKYGNGQMQSAVFLN
                     NSSFLLSQCEKKFSSFLNYSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWS
                     GVKAARAKPIPFLHNSVDSLKKRIEHHGPGIIAVDSIYSTLGTISPLADYADIARKFK
                     CLLIVDESHSLGTHGSQGKGMVAKLGLSSQIDIITASLAKALSGRGGIITGDRQLIEY
                     IRYSSLPSIFSSALMPHDLAGFSASLEIITQEEWRRAKLQTNADFLREALSCEGINIG
                     SSGSQIVPLVTGSEANTLWLRDELEKEDLFGAVFCSPATPKNKCLIRLSISAHHEKSD
                     LLRVIECLAKLARKRPDIPLFKGN"
     gene            4920..5348
                     /locus_tag="U6309_002332"
     CDS             4920..5348
                     /locus_tag="U6309_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEN5505765.1"
                     /translation="MRLNIALILSFFSIMLFAMDEVDEQQILQRIQPVGKVRVQEEQG
                     NNIEAGSTKTTVVDSKSKKEPGQDIYDKYCSVCHRDGLAGAPKFQSEADWKPRLASKK
                     IDDLLASAIKGLNAMPAKGTCMDCSDAELKAAIQYMLPKS"
     gene            5345..5836
                     /locus_tag="U6309_002333"
     CDS             5345..5836
                     /locus_tag="U6309_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEN5505766.1"
                     /translation="MKKLTYRKIQSFQAIITVLVIFASFYLEYAVGLQPCPLCLMQRV
                     CVFILLGLMGLSLGTVKKAHIVSLMQFMVACAGLYFSLRQLWLQSLPSDQAPACMPGL
                     DVLIQYFPWQTVAKALFWGAGDCAEVTWTMFGISMPGWAAMYFLFMAIMGLFLFFRTR
                     MIN"
     gene            5898..7049
                     /gene="tgt"
                     /locus_tag="U6309_002334"
     CDS             5898..7049
                     /gene="tgt"
                     /locus_tag="U6309_002334"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEN5505767.1"
                     /translation="MNTFSCEVLYQHSGTKARVTRVVTPHGEFITPVFMPVGTRAGVN
                     NMMPSELSAAGTQIILGGNTYHMLCAPGMKVIENAGGMHRFMGWHGPMLTDSGGFQVF
                     SLSKNKEICSIDESGAHFKLPGSKGVIHMTPEMSLETQKIIGADIIMAFDQCTPDSCS
                     KDEVSQIMLRTHRWLKQSIDYHQQYPNSRYGYKQALFGIVQGGVFQDLRRESADFVSA
                     MNTDGIAIGGETVGFDMNTTVEVIRWVYEYLPGNKPRYTMGVGMSPQDLLDVVAEGID
                     MFDCVAPTRNARHGALYCGKLVREGNWLKFQSEHENGRIQIKKSCFADDLAPVMESCS
                     CYTCLSYSRSYLHHLSKQKANLFTALASIHNVHVLHDVCNHMRHLIINS"
     gene            7076..7570
                     /locus_tag="U6309_002335"
     CDS             7076..7570
                     /locus_tag="U6309_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN5505768.1"
                     /translation="MVLISTSMGDIHIKLDTENTPLTAENFLNYVRKGFYNDTIFHRV
                     IDGFMIQGGGLKTNMEQKITASPIQNEAKGAKPNKRGTIAMARTMDPHSATAQFFINV
                     ADNGFLNYSGDHPQGYGYCVFGEVVEGMDVVDAIAKVKTGQRNGHADVPVEEISIIEV
                     RELA"
BASE COUNT         2295 a         1320 c         1615 g         2550 t
ORIGIN      
        1 ttattaaaat tttttttgtc tcgaccttgg agctttgtgt aagtcctata gagcatatct
       61 catgcacaat ccctatattt tatgataggc ttttccagta gataaagcgc tgaaaatcag
      121 gggcgttttt tagcttgatt taaggataag gaatgtcaca actaaaaaaa atagtgaaac
      181 atttagatga atcaatgcag cgtagtttgt ctaattctgc tcatcaattg gttgcagtag
      241 gcgcgatagc ttttgtggga tttcctttat tttatgttat ttgggctttc tggttgcccc
      301 agccttatga aaacctgcca ctccgcctta ttggaagcct tttaggtttg ggattgatgc
      361 ttactcctta ttggccatta aaatggaagc aatatttatc ttggtattgg tttttaactc
      421 ttttgtttac tttgccatat ttttttactt ttttattttt aatgaatcaa gcaagtgtta
      481 tttctgcgat gtctttgttg tgtggtgtat ttttacttgt attgctcgtt gatttattga
      541 gtttatccat tgtgctgata ctggggttta gtctagctct tgtaagttat tatcttgttt
      601 ctccgcaaat gtattttgga gaagagcata tacaaatgac attggtgatc atatttacca
      661 ttattgcagg ttctacttta aattataaaa cagccatgtt gcaacaacaa aaattggctg
      721 gcatggctgc tgctgcaggc atgattgccc atgagttacg ttcaccattg ttaggaataa
      781 agagcggcgc tcaagcttta gtccaatatt tgccaagatt gattgatggt tatcaattgg
      841 ctaaaaaaca agagttaata tcctcacctt tacgtgacag attgttatta cagcttgggg
      901 gagtcagcaa tcgaattgtt agtgaaattg attatgctaa tacgatcatt gatatgttat
      961 tagtcaaggc aagtcgcgat aatattttgc aaaattgcat attagagact tgttctatag
     1021 cagaatgttt gtctgaggct attaccagat acccatttca gtcaccaaga gaaagaacat
     1081 taatcacatg gaccggtgat tttcaattta aaggctccaa gttgttaatg cagcatattt
     1141 tatttaattt aataaaaaat gcactttatg ttatcgcaat ggcacagcgt ggggagatta
     1201 atatttggac tgaatctggc gataagtatc attatttgta ttttaaagac acagcaaaag
     1261 ggatgagcaa acaacaatta aatcgattat ttaatcattt ctatactact acctttatgg
     1321 gaacaggcat tggtttatca ttttgtaaat tagttatgaa tgcgtttggt ggtgatattg
     1381 tttgtgaatc taaagagggc gaatacgctt tgtttatact gagttttcca ggctcggttt
     1441 aaaattggtt ttctgttgaa cataccacca ttcgtcttaa aagacgtaag tatggtattg
     1501 agctagagag cccctgtgat tctcttctgc tagagtttgg ccgtttttac tgagatagcg
     1561 ataaaaatat atgtccaacc agtgacgatc aattcaatcg caataaaaag cccaatgatc
     1621 caaagcccgc taacaggcca ttgcattaga attaataccc caagaataat ggcggtgatt
     1681 ccggccaata ataaccatcc ccatcctttg gagtctttta aggcaaaagc cataatcatt
     1741 cttgttaaac caattacaat aagcaccgct gccagaaaag ctgtgattag agtggatgct
     1801 aaaaacgggt catacaaaac aatgcatccc gccgcaatat acaacactgc aataagcgcc
     1861 tgccatatca ttcctttcca ctctttgtat ttaaatacgt ctataatgtg acttattccc
     1921 ccaacaatta ataaggcacc aaaaaagaac atacttacca aggttagacc aactaccatt
     1981 ccaagaccaa cacagccaag gataaggaaa aatattccaa atcctaaaat ccatccccag
     2041 tttcgtttta actcatccac tgggggttgg taaaattctt gtgaattagc catatcaata
     2101 tccttttttg atttcttagc taagataagg tacttattaa taatatagac ttatattaaa
     2161 taacttgtct aattttgagc tttgatttta agaactctta ttttgaatac tcactccaaa
     2221 aagcccggtg aatataaaac gtaaataatt tggttataat aaacaatcat gttactttat
     2281 tcacaagtta gccaggttct gatgatggag ccacaggtta tttgaagttc atagaaagga
     2341 tataactttg gaacgttttg ctcaggagga gctatgcaac atttctcaat acctacctgt
     2401 tactttccaa gcactgcggt ttttgtcgac gacagtcggg atttcttgct caatttcgta
     2461 ttgcagctgg atgagggttt ggcgtaccgt gtatttgatt ccccttttga agcgttggat
     2521 tgtattaaac aaaaacgctg tgaactggaa ttattaagtg aacgttgtct cagcgaatac
     2581 acagaagcaa aaaattgccc attaactaat cataccatta atcttgattt ggccgctatt
     2641 catgctgagg tttataactc tcgacgattt tctgaaattt cagtagtcgt tgttgattat
     2701 gcaatgccag gcatggacgg attagagttt tgcaggcgaa tcgaagatac taatattaaa
     2761 aaaatattat taactgggca agcagatgag aagctggcaa ttgctgcttt taatgaagga
     2821 ttaattcata gatacatcaa aaagagtgat ccggatgtcg ccagtctcat tactcaaagt
     2881 atccatgatt tgcagttaca atattttcaa agcatgtctg atatgattgt acgtatgctt
     2941 tcggtgacat ccccaaattg tttacatgac aaaaaatatg ctgagttatt ctggcgttta
     3001 tgcagggaaa agggaatcgt tgaattttat ctggccgata actcaggtag ctttttgatg
     3061 ctggatgatg atgccaacat cagtttttta atcgtgaaaa acgaagcgga tatgcagtta
     3121 cactatgatt tggctttgga taatggagct agtggagatg tattggacca attgcataac
     3181 ggagaaaaaa ttccttgttt ttggcaatct aatttagtga cacctcaatg gaatgagtgg
     3241 tcgaattgtt tggttcctgc caaccgattt gtttctgatg aaacctattt ttatgcttat
     3301 gtgcaagggg ctgttttgtt tgatgtgcgc ctggataaaa ttctttctta tcaccaatac
     3361 ctggaagagc tggatgcaga agaaatgttt ctgaattaat aggaattcta tataaagagc
     3421 acataattct tatttgaaat ggtaactgtt caaatgagct gccaataatt agaggtcctg
     3481 ttaattaccc ttaaacaaag gaatatcggg tctttttctt gcaagtttgg ccaggcactc
     3541 aatcactctg agcaagtccg atttttcgtg atgcgcactg atggataaac gaatcaggca
     3601 tttatttttg ggagtggcgg gtgaacagaa cacggctcca aagaggtctt ctttttctaa
     3661 ctcatccctt aaccaaagcg tgttagcctc gctgcctgta actaaaggga ctatttgaga
     3721 gccactactt cctatattaa ttccttcgca tgataaggct tctcgtaaaa aatcagcatt
     3781 ggtttgtaat tttgctcttc tccattcttc ttgagtaata atttctaatg atgcgctaaa
     3841 tcctgctaaa tcatgaggca ttaaagcaga actaaaaata gaaggcagag atgaatatct
     3901 gatatattct ataagttgcc tatctcctgt aatgatgcct ccacgtcctg ataaggcttt
     3961 agccaggctt gctgttatga tgtctatttg gcttgataat cccagtttag ccaccattcc
     4021 cttgccttgc gaaccatgcg tacctaatga atgagattca tcgacgatga gtaaacattt
     4081 gaattttctt gcaatgtcag catagtcagc caatggacta atcgtaccca gcgtactata
     4141 aatagaatct actgcaatta ttcctggtcc atggtgttca attcgttttt ttagtgaatc
     4201 gactgaatta tgtagaaacg gtataggttt ggctctggct gcttttacac cagaccaaaa
     4261 agacatatgg gcataaaaat ccagatacac tggtgtattg tacttggcca gggtttgtat
     4321 gagaccaacg ttagcactcc agcctgattg agtgagcagg gccgcagaat aatttaaaaa
     4381 ggaactgaat ttcttttcac attggctgag caaaaaagag ctgttattta aaaatacagc
     4441 ggattgcatt tgaccattac catatttttg catcgcagca atttgcgctt taattaattg
     4501 cggatgttga ctgatgttta aataatcatt gctcgaaaaa atcagtgaat ttgcagtcgg
     4561 ggttttgcct ttaagtatgt gtttcccttg ccaggattta ttgactcgcc cctcgtaata
     4621 tttttccatg gaatgattga tatactcagg ataagcatgg ttttgatgtt tttcttttcc
     4681 attgatatat tgagtctcgt tatcaattgt ttggtattct gtattgctca tccataactc
     4741 agattctttt cccttgtgtg cagtttagca caatggttga tagtggaagg atcttttcag
     4801 taaaagtcta agtattaagt taatgatttt ataaatcgtt gggcttattg aaatctttct
     4861 ggaaaaaaaa gtcacttgtc ggtatgattt ttaaattaac tattagagga cggataaata
     4921 tgaggttgaa tattgcgctg atcttgtctt ttttttcaat catgcttttt gcaatggacg
     4981 aggtggatga gcaacaaatt ttacaaagaa ttcaaccggt agggaaagtt cgagttcaag
     5041 aggaacaggg gaataacata gaagctggat cgactaaaac gactgttgtt gattccaaga
     5101 gcaaaaaaga accaggccaa gatatatatg ataaatattg ctccgtatgt catagggatg
     5161 gattggcagg ggcgcctaaa tttcaatcgg aggccgattg gaaacctcga ttggcaagta
     5221 aaaaaataga tgatttactc gcttctgcaa tcaagggatt aaatgcaatg ccagcaaaag
     5281 gtacttgtat ggattgttct gatgctgaat taaaagccgc aatacaatat atgttgccga
     5341 aatcatgaaa aagcttactt acagaaaaat acaatctttt caggcaatta ttacagtatt
     5401 ggtaatattc gcttcgtttt accttgaata tgctgttggg ctacaaccat gtcctttgtg
     5461 cttgatgcaa cgagtttgtg tatttatttt gttaggatta atggggttgt ctctgggaac
     5521 tgtgaaaaaa gcgcatatag taagcttgat gcagtttatg gttgcgtgtg ccggtttgta
     5581 tttctcttta aggcaattgt ggttgcagtc tttaccatca gatcaagctc ctgcttgcat
     5641 gcctgggctt gatgtattaa ttcagtattt tccatggcaa actgttgcta aagccctatt
     5701 ttggggagca ggggattgtg ctgaagtgac ttggactatg tttggaattt ctatgcctgg
     5761 gtgggctgcc atgtattttc tgtttatggc gataatgggg ctttttctgt tttttcgaac
     5821 ccgaatgatc aattaattcc tggcattaaa cgcgtgcttt cgttatcatt ctttgtttta
     5881 tcttgtcgtt tctgcgaatg aacacttttt catgtgaagt actgtaccag cattcgggta
     5941 ccaaagctcg tgttacacgt gtggttacgc ctcatggtga atttataacc ccggtattta
     6001 tgccagttgg aacgcgtgca ggggtcaata acatgatgcc gtcagaacta tctgctgcgg
     6061 gtactcaaat tattctggga ggtaatacct atcatatgtt gtgtgcgcct ggcatgaagg
     6121 tgatagagaa tgcagggggg atgcatcgct ttatggggtg gcatggacct atgctaacag
     6181 acagcggtgg ttttcaagtt tttagtttat cgaagaacaa ggaaatttgt tctatagatg
     6241 aatctggcgc tcattttaaa ttgcctggca gtaagggggt tatccatatg accccggaaa
     6301 tgtctctgga gacccagaaa ataattggtg ccgatattat aatggcattt gatcagtgta
     6361 ctcctgacag ctgctccaag gatgaagtaa gtcaaatcat gttgcggacc cacagatggc
     6421 taaaacaatc gattgactac catcaacaat atcccaactc tcgatatgga tataaacaag
     6481 ctttatttgg cattgtgcaa ggaggagttt ttcaagattt acgtcgagaa agtgctgatt
     6541 ttgtttctgc aatgaatacg gatggtattg caataggtgg agaaactgta ggctttgata
     6601 tgaatactac agtggaagtc attcgttggg tgtatgagta tcttccaggc aataagccac
     6661 gttatactat gggagttgga atgtcacctc aagatctgtt ggatgtggtg gcagaaggta
     6721 tcgatatgtt tgattgtgtt gcaccaacac gtaatgcccg gcatggtgcc ttatattgtg
     6781 gtaaattggt aagagaaggg aattggctaa aatttcaaag cgaacatgaa aatggacgaa
     6841 ttcaaataaa gaaatcatgt tttgctgatg atttggcacc tgttatggag agttgttctt
     6901 gttatacttg tcttagttat tcaagatcct atttgcatca tttatcaaaa caaaaagcta
     6961 atttatttac tgctttggca agcatccata atgttcatgt tttgcatgat gtttgtaatc
     7021 acatgcgtca tttgataata aattcctaaa ttatttaata ttaagaggtg aaataatggt
     7081 tttaatttct acatccatgg gtgatattca tatcaaatta gatacagaaa ataccccatt
     7141 aactgcagaa aacttcctaa attatgtgcg taaaggtttt tataatgaca cgattttcca
     7201 tcgtgttatc gatggtttta tgattcaagg cggcggtttg aagacaaata tggagcaaaa
     7261 aataactgcc tctccaattc aaaatgaagc aaaaggagcg aaacctaaca aacgtggaac
     7321 catcgcaatg gcgagaacaa tggatcccca ttctgcaaca gcccaatttt ttattaatgt
     7381 tgcggataat ggttttctga attacagcgg cgaccaccct caaggttacg gttactgtgt
     7441 atttggagaa gtagttgaag ggatggatgt ggttgatgct attgctaaag tgaaaacagg
     7501 ccaacgaaat ggtcatgcag atgtccctgt agaagaaata agcatcattg aggttagaga
     7561 attggcttag cgagaaaata tatggctgaa atagcatagg ttttccatgg caaaagtata
     7621 ctttctttaa tttcatcccc agtatggaag agggatcttc cttatgcgtg caggaagctg
     7681 catttgcaaa acagagtgcc atttttcaga ggcaaaatat catggggtat ctctaagaga
     7741 ttaacatcat tatttaagtg ttttaatgaa aaaataacgt
//
LOCUS       DASLOE010000142         7762 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.143, whole genome shotgun sequence.
ACCESSION   DASLOE010000142 DASLOE010000000
VERSION     DASLOE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.143"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(110..2182)
                     /gene="recG"
                     /locus_tag="U6309_002336"
     CDS             complement(110..2182)
                     /gene="recG"
                     /locus_tag="U6309_002336"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEN5505769.1"
                     /translation="MLSNSCEYLTGVGPTIAAKLAKCGIFTIQDLLFHLPYKYQDRTR
                     ITPIQDLRSNEWCVIAGHVCKTEIKYGKRMMLNCYVEDKTGVVNLRFFHFNKQQIQAL
                     NNSTMIRAFGEVRGFNNQLEMIHPEYQLIDQESDFHVEETLTPIYPSTQGLTQARLRQ
                     LVKIALEQSEHELHQLEWMSEKQLLENNFYDLGEAIKLLHNPPPDISLSNLEAGEHPA
                     LKRLIFDELLAQQLSMQFARQSRSKLQAPAILFDNAIHKRFIESLPFSLTNAQQRVFK
                     EISVDLTQSKPMLRLLQGDVGAGKTIIAALAALQAISQGFQVAFMAPTDLLSEQHTNS
                     LSKWLEPIGINVLRLSGKMKTTERKNALTALQDNSCQLIVGTHALFQEQVEFARLGLV
                     IIDEQHRFGVEQRLLLQQKGQLNQLIPHQLLMTATPIPRTLSMSHFAHLDISVIDELP
                     PGRMPITTAVLNQDKRELIIERLQAAIANGKQAYWVCTLIEESEKLQCIAATDTSKKL
                     QEQLSFARVGLVHGRMKALEKEATMAAFKQGEIDLLVATTVIEVGVDVPNASLMIIEN
                     AERLGLSQLHQLRGRVGRGNNQSHCLLLYQSPLSQQGAERLKIMRSTTDGFLISEKDL
                     ELRGSGEILGTRQTGFRQFKIANLQRDKTLFAILRPIAKQLVLEKTAIARQITQRWLG
                     NFEQFLQS"
     gene            2329..3387
                     /locus_tag="U6309_002337"
     CDS             2329..3387
                     /locus_tag="U6309_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN5505770.1"
                     /translation="MNRIITFTAGLILVWITGYLLIAGRGLLIPIVISIFVWHLLNTI
                     NSGVQKIPFIGTHLPSWIRMILSLFVVVCLVMVLINIITNNVSDVIAASPRYQENLLH
                     IFNSLDQRFHVKGIFDFDSLIQGLSIQSMLVNIYGVFTTITSSAVLISLYVVFLFVEQ
                     HYFTQKMDAFFPQAEHRKLVKNIISHITKDTQTYLGIKTLLSLLTATLSWFIMKWVGL
                     DFAEFWALLIFFLNYIPNIGAIVATAFPAALALIQFESWWPFLEVTSGIVAIQFFVGN
                     FLEPRLLGKSLNLSPLVILFALALWGAIWGVLGMFLSVPITVMMMIIFAHFDVTRPIA
                     ILLSQDGYIHKSYESLEN"
     gene            3491..5260
                     /locus_tag="U6309_002338"
     CDS             3491..5260
                     /locus_tag="U6309_002338"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEN5505771.1"
                     /translation="MRSMKAIIEYLLKQALINLQQSGEMPIDLEIEIKVENAKDPAHG
                     DYATNLALVLAKPCRQAPKVLAERLVAVIPADPSVEKIEIAGAGFINFFMRSTARSLI
                     ISEILNKGKEFGRGNLGQSQKVLIEFVSANPTGPLHVGHGRGAAFGATLGNVLKAAGY
                     DVTLEYYVNDAGRQMNILAVSVWLRYLELAGEPIVFPANGYKGQYVYEIAQEMWSEQG
                     NQLVHPWISVVENLPADEPEGGDKETYIDAIIARAQSLLGKDGFANFHQHALKTVLDD
                     IKDDLQAFGVRFDSWFSEQSLFEDGSIEKGIQALKDRGHTYEREGALWFRATDFGDEK
                     DRVLVRANGQTTYFASDVAYHWNKYDRGFDRVIDIFGADHHGYVTRIKTAVKALGHDE
                     SALDVILVQFAILYRGGDRVQMSTRSGSFVTLRELREEVGNDAARYFYVARKPEQHMD
                     FDLDLAKSESSDNPVYYIQYAHARICSVLRQLKERGLKWDKDMGLKNLDLLEQQHETA
                     LISLIARYPEVIQSAAASCEPHQLAYYLRELANGLHSYYNAIQLLCEQEQLRCARLCL
                     LESVRQVLNNGLAILGVSAPESM"
     gene            5260..5955
                     /locus_tag="U6309_002339"
     CDS             5260..5955
                     /locus_tag="U6309_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEN5505772.1"
                     /translation="MAKDYGNRRAARRNRGPHQFLVVIVTFLFGYITASFLDVQTISQ
                     WVNTQVLAQHDDEKAPTKVVQEHKQVPPKPKFEFYTLLANERGPASQASSQQNTDNHA
                     ADKTSTPIQKTANTVIVAVNNSVKSTIANPTSQTPVKVAEAKPTTMQQPNKGAYLVQV
                     ASFKARQDAEQMKGMLILKGFDVSVVPVTQAQGNWFRVVVGPYPNKALAQKAQMNLAK
                     TEHLNGMVRSVGG"
     gene            complement(5995..6333)
                     /gene="folB"
                     /locus_tag="U6309_002340"
     CDS             complement(5995..6333)
                     /gene="folB"
                     /locus_tag="U6309_002340"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEN5505773.1"
                     /translation="MDTLNITALSVETKIGVYGWEQRINQRLLIDISIPFDFKDCKDN
                     LSNTLDYADLCQTVTQFVESTSFQLIETVAEQIAQLIKQKYPVSKVSVAVTKPHAVKN
                     AAGIQVVVNR"
     gene            6573..7631
                     /locus_tag="U6309_002341"
     CDS             6573..7631
                     /locus_tag="U6309_002341"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEN5505774.1"
                     /translation="MFDLNQSLFLRALRRQPVERTPIWIMRQAGRYLPEYRKVREHAG
                     DFLNLCKNPELACEVTLQPLRRYALDAAILFSDILTIPDAMGLGLYFAEGEGPCFTNP
                     LQDTKAIHALKIPSIPESLSYVFDAARLIRQEMPKELPLIGFSGSPWTLACYMVEGGS
                     SRDFKRILNLIYTEKEAAHLLLNKLAISVTAYLIEQIKAGVNAVMIFDTWGGVLTPQN
                     YKDFSLAYMHQIVQQLKKEYPDIPVILFTKNGGQWLEWMAETGCDALGVDWTCDLASA
                     RKRVGGKVALQGNLDPAVLLTAKNCIRREVGSVLASYGYGTGHIFNLGHGINPDVPPE
                     NVAIMIEAVHEISPQYHL"
BASE COUNT         2203 a         1376 c         1704 g         2479 t
ORIGIN      
        1 taaagttatc cacagggata agagggaatg aattttattt ctggcagcct tatgttagca
       61 aagaaaaaaa taaaaaacag tcttgacttc aataatttta attatttttt taactttgca
      121 aaaattgttc aaaattgccc agccatcgtt gtgtaatttg tctggcgatt gctgtttttt
      181 ccagcaccag ttgttttgca attggacgta aaatagcgaa gagggtttta tcgcgttgta
      241 agttagctat tttaaattgt ctaaagccag tttgtctggt tcctaatatt tctcctgagc
      301 ctcttaactc caaatccttt tcggagatta gaaaaccgtc tgttgttgag cgcatgatct
      361 tgagacgttc agcaccttgc tgtgatagag gagattgata aagtaataga caatgagatt
      421 gattattacc tctgccgacg cgcccgcgta attgatgtaa ctgcgacaaa ccaagtcgct
      481 ccgcattttc aatgatcatt aaactggcat taggaacatc tacaccaact tcaataactg
      541 tagttgccac caataaatca atctcgcctt gtttaaaggc cgccatagta gcttcttttt
      601 caagcgcttt cattctccca tgaacaagac caacacgcgc gaaagagagc tgttcttgta
      661 atttttttga ggtatctgtt gctgctatgc attgtaattt ttcagattct tctataaggg
      721 tacagaccca gtaagcttgt ttgccgttgg cgattgctgc ttgcaaacgt tcgataatga
      781 gttcacgctt atcctggttc aataccgctg ttgtaattgg catacggcca ggtggtagtt
      841 catcgatgac cgatatatcc aaatgagcaa aatgagacat tgacagggtt cttggaatag
      901 gggtggctgt cattaataat tgatgaggaa ttaattggtt taattgccct ttttgttgca
      961 gaaggagccg ttgttctact ccaaatcggt gttgttcgtc aataatgacc aaacccaaac
     1021 gagcgaattc gacttgctcc tgaaataagg catgagttcc aacaatgagc tgacagctat
     1081 tgtcctgcag ggctgtaagc gcattttttc gttctgtggt tttcatttta ccgctcagtc
     1141 ttaatacatt aataccgata ggttctaacc atttactgag gctatttgta tgttgttcac
     1201 ttaataaatc ggttggtgcc ataaaagcaa cctgaaatcc ttgtgaaatg gcttgaaggg
     1261 cggctagtgc cgcgataatg gttttacccg ccccaacatc tccttgcagc aaacgaagca
     1321 tgggtttgct ttgagttaaa tcaacgctga tttctttaaa aacccgttgt tgtgcgtttg
     1381 taagactgaa tggcaatgat tcgataaaac gtttatgaat tgcattatcg aaaagtatag
     1441 cgggagcttg gagtttgctg cgtgactgtc ttgcaaattg catactgagc tgctgtgcca
     1501 gtaattcatc aaatattaac cgcttgagag caggatgttc gccggcttcg aggttggaga
     1561 gggagatatc aggtgggggg ttgtgtagta atttaatggc ttcgcccaaa tcataaaaat
     1621 tgttttccag taattgtttt tcactcatcc attccaattg atgaagttcg tgttcagatt
     1681 gttccagggc tattttaact aactgcctta atcgggcttg agtcaagcct tgagtcgatg
     1741 gataaattgg cgtaagcgtt tcttccacgt ggaaatctga ttcttggtca attagttgat
     1801 attctggatg aatcatctcc aactggttgt taaatccgcg gacttcgcca aaagctcgaa
     1861 tcatggtgct attattcaaa gcctgaattt gctgcttatt gaaatgaaaa aaacgaaggt
     1921 taactactcc tgttttatct tccacataac aattcaacat catcctcttg ccgtatttta
     1981 tttcagtttt gcatacatgc cccgcgataa cgcaccattc attggagcgc aaatcctgaa
     2041 ttggtgttat cctggttctg tcttgatatt tataaggcag gtggaaaagc aagtcttgta
     2101 tcgtgaaaat tccacattta gcaagttttg ctgcaatggt tgggccgacg ccagttaaat
     2161 attcacaaga gttactcagc acggtattat ttaagattaa aaatttaata ataacaggaa
     2221 aatatattcg ttgcgacttg agtcatcatt ctggcaattt tgcccaaatc attactaggt
     2281 tacttgttgc ttgcgagtat ctattcatca aagatggagt gtaattggat gaatcgtatt
     2341 ataacgttca cagcgggttt gatactagtt tggataacag gttatttatt aatcgcagga
     2401 agaggactat taattccaat tgttatatcc atttttgtct ggcacctgtt gaataccatt
     2461 aatagcggtg ttcagaaaat tcctttcatc ggcacacatc tcccatcctg gataaggatg
     2521 attttatctt tgtttgttgt tgtttgcctc gtaatggttc ttattaacat cattaccaat
     2581 aatgtcagtg atgtgatagc cgcttctccc aggtatcagg aaaatttact gcacatattt
     2641 aacagcctgg atcaacgatt tcatgttaag ggtatttttg atttcgacag cttaattcaa
     2701 gggttaagca ttcagagtat gcttgttaat atttatgggg tttttacgac aataaccagt
     2761 tccgctgtac tgatttcctt atatgttgtg tttctgtttg tcgagcagca ttattttact
     2821 caaaagatgg atgccttttt ccctcaggca gagcacagga agctggttaa aaacattatc
     2881 tcccatatta caaaagatac tcaaacttat ctgggtatta aaactttgtt gagcctactc
     2941 actgccacgt tgagttggtt tattatgaaa tgggttggat tggattttgc cgagttttgg
     3001 gcattattaa ttttcttttt aaattatatc cccaatatag gagctattgt agcaacagct
     3061 tttccagctg cgcttgcttt gatccaattt gaaagctggt ggccgttttt ggaagtgact
     3121 tccgggatag tggctataca gtttttcgtg ggtaattttc tagagcctcg tctcttaggt
     3181 aaatcactaa acttaagtcc cttagtgata ttgtttgcct tagcgctatg gggtgccatt
     3241 tggggggtac taggcatgtt tttatctgtg cctatcactg taatgatgat gattattttt
     3301 gcgcactttg atgtgacaag acccatagca attctgctgt ctcaagatgg atatattcac
     3361 aaatcctatg aatctcttga aaattaatga gctattgctg tttctattgt tgtagtgatg
     3421 gtgcttctgg gttgagagcc tttccatatg gtatgaaaaa gagcataata tcggtatttt
     3481 tgacggataa atgagaagta tgaaagctat aatcgaatat cttttgaaac aggcattaat
     3541 taatctgcaa caatcaggcg aaatgccaat tgatttggag attgaaataa aagttgagaa
     3601 tgccaaagat ccagctcatg gtgattatgc gactaatttg gcactggtat tagctaaacc
     3661 atgccgacaa gcacctaaag tattagctga gcgattggtt gcagtaatcc ccgcagatcc
     3721 gtctgtagag aaaattgaga tagccggcgc aggatttatt aattttttca tgcgcagcac
     3781 ggcacgctcg ctcatcattt ctgaaatttt gaataaaggc aaggagtttg gccgaggtaa
     3841 tttaggacag agccaaaaag tattgattga atttgtttct gctaatccaa cagggccttt
     3901 acatgtagga catggaagag gagctgcttt cggagcaact ttgggtaatg ttttaaaagc
     3961 cgctggttac gatgtgactt tggaatatta tgtcaatgat gcgggcaggc aaatgaatat
     4021 tctcgctgtg agtgtgtggc tgcgctacct ggaattggca ggtgagccaa ttgtttttcc
     4081 ggcgaatggc tataaaggtc aatatgttta tgagattgca caagagatgt ggtcagagca
     4141 gggtaatcaa ttagtgcatc catggatttc ggtagtagaa aatttacctg ctgatgaacc
     4201 cgaaggtgga gataaagaga cttatatcga tgcgatcatc gcgcgagcac aatccttact
     4261 gggcaaggat gggtttgcta atttccatca acatgcttta aaaacggtat tggatgatat
     4321 aaaagatgat ttgcaggcgt ttggtgttcg ttttgacagt tggttttctg agcaatcgtt
     4381 atttgaagat ggttctattg agaaaggtat tcaggccttg aaagatagag gccacactta
     4441 tgaaagagaa ggcgctttat ggtttagggc aactgatttt ggtgatgaaa aagatagagt
     4501 attagtccgg gctaatgggc aaacgacata tttcgcttct gatgttgcat atcactggaa
     4561 taaatatgat agaggttttg atcgagtcat tgatattttt ggtgctgatc atcatggtta
     4621 tgtcacgaga attaaaacag ctgttaaggc attaggacat gatgaaagtg cattggatgt
     4681 cattttggtg cagtttgcca ttctgtatcg aggtggtgac agggtgcaaa tgtctactcg
     4741 cagtggttca tttgttactt tgagagagct aagagaagaa gtaggaaatg atgcggctcg
     4801 ttatttttat gtggcaagaa aacctgaaca acacatggat tttgacctgg acctggcaaa
     4861 gtccgaatcc agtgacaatc ctgtgtatta tattcagtat gcacatgccc gtatctgcag
     4921 tgtattgaga cagttaaaag aaaggggttt gaagtgggat aaggatatgg gacttaaaaa
     4981 ccttgattta ttggagcaac aacatgagac cgcattaatt tctctgattg cccgatatcc
     5041 cgaagtcatt caatctgccg ctgcttcttg cgagccgcat cagcttgctt actatttaag
     5101 agagctagcc aatgggttac acagttatta taatgcgatc cagctattat gcgaacaaga
     5161 acaattgcga tgtgcccgtt tgtgtttgct ggagtcggtt cgacaggttt taaataatgg
     5221 tttggctata ttaggcgtat cagctcctga gagtatgtga tggcaaaaga ttatggaaat
     5281 aggcgtgctg cacgtcgcaa cagaggaccg caccagtttt tggttgttat tgtgactttt
     5341 ttgtttggat atataaccgc ttcgtttctc gatgtacaaa cgataagtca atgggtgaat
     5401 acacaggttt tagctcaaca tgatgatgaa aaagcgccaa ctaaagtggt gcaagaacat
     5461 aaacaagtgc ctcctaagcc taaatttgag ttttatacat tattagcgaa tgaaaggggg
     5521 ccggcatctc aggcatctag tcagcaaaat acagacaatc atgcggcaga taagacatca
     5581 actccaatac aaaagactgc aaatactgtc attgttgctg taaataattc agtgaaatca
     5641 acgatagcta atccaacatc gcaaacccca gtgaaggtag cggaggcaaa accaacaacc
     5701 atgcaacaac ctaataaagg agcatatttg gttcaagtcg catcttttaa agcgcgtcag
     5761 gatgcagaac aaatgaaagg gatgctaatc cttaagggtt ttgatgtcag cgtggttcca
     5821 gtcacccaag cacagggaaa ttggttcagg gtagtggtgg gcccctatcc aaataaggcc
     5881 ttggctcaaa aagctcaaat gaaccttgcc aaaacagagc acctgaacgg catggtgcgc
     5941 agcgttggcg gataaggagt aatcatttaa tcaaagaaga ttgcttcttt cagattatct
     6001 gtttacaact acttggattc ccgcagcgtt ttttacagca tggggttttg taacagcgac
     6061 ggaaacctta ctaacaggat acttttgctt gattaattgt gcaatttgtt ccgctaccgt
     6121 ttcaattaat tgaaaagaag ttgactctac aaattgagtt actgtttggc acaaatcagc
     6181 gtaatccagt gtattagaca aattatcttt gcaatcttta aagtcaaagg gaatggatat
     6241 atcaattaat aatcgttgat taatacgctg ctcccaccca taaacaccaa tttttgtttc
     6301 aacactgaga gctgtaatat ttaaagtatc catttgatat ccattaataa tttatcagga
     6361 tttacgaaaa atcctcaatc tttttgttta aaatttttta actcagtcat ttaatttatc
     6421 taaactttct tatgtaaata ttgtttgcct tgagcttgga gtttttcgtg tgccctgctt
     6481 atattgttaa gtatcgattg ctatttatac cactgtattg attaccttaa catgatacta
     6541 taaacacttt ttattgtgat agtgattaaa tgatgtttga tttgaaccaa tccctttttc
     6601 ttcgagcttt gagacgacaa cctgtggaga gaactccaat ctggattatg cgccaggctg
     6661 ggcgttattt gcctgaatat agaaaagtaa gagagcatgc tggtgatttt ttaaatcttt
     6721 gcaagaatcc ggaactcgcc tgtgaagtaa ccttgcaacc gttgcgtcgc tatgctttgg
     6781 atgcagctat tttattttcc gatattttaa caatacctga tgctatgggg ctagggctct
     6841 attttgccga aggagaagga ccttgtttca caaatccttt acaagatacc aaggcaattc
     6901 atgccctgaa aattccttcc attcccgaat ctttatccta tgtctttgat gctgcgcgct
     6961 taatccgcca ggaaatgcct aaagaattac ctttgatcgg attttcaggt agcccatgga
     7021 ctttagcttg ttacatggtt gagggtggga gcagtagaga ttttaagcga attttaaatt
     7081 taatatacac tgaaaaagaa gcagcgcacc tgttattaaa taaattggct atctctgtga
     7141 ctgcctattt aattgagcaa ataaaggcag gggttaatgc ggttatgatc tttgatacgt
     7201 gggggggagt tttaactcca caaaattata aggatttttc tttggcatat atgcatcaaa
     7261 ttgttcagca attgaaaaaa gaataccctg atatccctgt aattttattt accaagaatg
     7321 gtgggcaatg gttagaatgg atggcagaaa ctggttgtga tgcattagga gttgactgga
     7381 cgtgtgattt ggcttccgca cgcaagagag taggcggaaa ggttgcttta caaggaaatc
     7441 tggatcctgc tgttttacta actgcaaaaa attgcatacg aagagaggtt gggagtgtac
     7501 tggcatctta tggttatgga acagggcata tctttaattt gggccatggc attaatccgg
     7561 atgtaccccc tgagaatgtt gcaataatga ttgaggcggt gcatgaaata agtcctcaat
     7621 atcatttgta gcctgtgcga ggcaaattat tgtttcaaaa tagtgaaatg cctcaagaat
     7681 aagacaatcg taactgtaca cggggtatgc aagtttttct gtttataagc ctatttattt
     7741 gagttgtctt tcccgcgcag gc
//
LOCUS       DASLOE010000143         7734 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.144, whole genome shotgun sequence.
ACCESSION   DASLOE010000143 DASLOE010000000
VERSION     DASLOE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.144"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(134..4633)
                     /gene="sdeA"
                     /locus_tag="U6309_002342"
     CDS             complement(134..4633)
                     /gene="sdeA"
                     /locus_tag="U6309_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector NAD-dependent ubiquitin ligase
                     SdeA"
                     /protein_id="HEN5505775.1"
                     /translation="MPKYVEGVELTQEGMHAIFARMGYGDITSGSIYNGVPTIDTAAL
                     NRQGFMPVLTGVGPHRDSGHWIMLIKGPGNQYYLFDPLGKTSGEGYQNILAAQLPMGS
                     TLSVIPNDSGLNMGLCGYWVASAGLRAHQALNQPIPPTLLNLGQTITDEMRNELDHDG
                     YRKITGWLRAVADEFPHGDEQFDAKALRENTEKDLKIEIPTLVLPGKDTSPKETPVKP
                     TAPQDKSVPVWNGFSLYTDDTVKAAAQYAYDNYLGKPYTGPVESAPANFGGRMVYRQH
                     HGLSHTLRTMAYAELIVEEARKAKLRGETLGKFKDGRTIADVTPEELKKIMIAQAFFV
                     AGRDDEASDAKNYQKYHEQSRDAFLKYVKDNESTLIPDVFKDQEDVNFYARVIEDKSH
                     DWDSTPAHVLINQGHMVDLVRVKQPPESFLQRYFSSMQRWIGTQATEAVFGIQRQFFH
                     ATYEVVAGFDSDNKEPHLVVSGLGRYVIGEDGQPIREAPKKGQKEGDLKFFSQTYKLK
                     ENERFMRVDEFLKLPEVQSNFPGAGKHLHGGMPGMNEMDYWNRLNSLNRARCENDVDF
                     CLKQLQTAHDKAKIDPIKQAFQSSKEKGRRQPNVDEIAAARIIQQILANPDCIHDDHV
                     LINGQKLEQQFFRDLLAKCEMAVVGSLLNDNDIGNIDTLMRHEKDTEFHSTNPEAVPV
                     KIGEYWVNDQNQHNRSNNITQKKHDLIFLMQNDAWYFSRVNAIAQNRDKGSTFKEVLI
                     TTLMTPLTSKALVDTSHAPAPKRLFRGLNLPEEFTKGLIDQANTMIANTTERLFTDHS
                     IEAFKQIKLNDLSKISSRTNASTTTDIKLVTETWDSNVIFEMLDPDGLLHPKQVGRHG
                     EGTESEFSIYLPEDVALVPVKVTLDGKTRQGENRYVFTFVAVKSPDFIPRHESGYAVE
                     PFLRMQTAKLAEIKSSIEKTQVAPDLEKIFNLQNEVEAVQYSHLSTGYKNFLKNTVGP
                     VLENCLNGFMESDAVALSKALAAFPSDGQWSAFNFEEARQAKRQMDALKQMVANKVVL
                     DALTQCQEALEKQNIAGALDALKNIPSEKEIGTIRRELREQIQSTRQELESLQRAVVT
                     PVVTDEKKVRERYDALIENTSKKITELETGKLPNLDAVKKGISNLSNLKQEVTVLRNE
                     KIRMHVGTDKVDFSDIEKLEQQIKFMDTKLADAYLLEVTKQISALENEKPKNQTELKT
                     KIAAFIDRTADIETLRNDRIKKHGSSKDPLDLSDLDKLSGNLDRINQSIVSSLINTIR
                     VSISQMEAKTFHMQEKEIQQNFELLAKLEKTLDKSKTSEKLREDIPKLNDLLVAKQKA
                     YPQMVQLQLKSEVFVTQLREICQANYDDLDKTRNARLRELDRLDREAGITRMVGNLLW
                     GFTNKVGLTTDERLDIRTKQQSLARFRTELFNDKIDTDQLISNLARKRPSELQEGLGI
                     SPDNAMDLYMVLTNLESKTTSPEKIEERMKAIDDISTKIGREPEHLKFVMVEEDESNK
                     KTIGF"
     gene            complement(5005..>7734)
                     /locus_tag="U6309_002343"
     CDS             complement(5005..>7734)
                     /locus_tag="U6309_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HEN5505776.1"
                     /translation="AKFPTDKQWSAFKSGEAVRAKAQMDVLKQQIEKKIILQSQIIPA
                     LTECSEALDKQNVTEALQALNKLPAEKEIGKVKTIGQELRGQIVGVKQELTGNLESMQ
                     RATTAPIVQDAEKMKVRYETLVTDVTKRVTDFEKIKPANLDSYNKAIADLNNMQQELT
                     LLRNEKIRMHTDKDKAVDFSDIEALEKRLQEAQPNLYPSLEEGTIKGIQELEKIPKSI
                     SFDDIKSMTSKINGYLETLEFIRNERVKKHGESTEPLDMSDLDKLKNQLQGVSQHLAQ
                     ILLNGAKNSLDKIKDPATLEKEGQYIKRCLDHFAGLEKTLDGSEKAIKQKEDFNTYKN
                     SLTEKQEKAYPEMLQLQYKSEALITQLRNLCNIHHDNLAKTREEQQQLLNKTGGILDG
                     FFSQITTAISQAEVKKANELARFKTELNNDKNDVSQLIQFLVKKKPSELEENLGISKE
                     NAEQLHGLLKQLDAKATPIDKLQENTKLIDEISTKIGSKPVSLEHVAATKAREYYQFL
                     LYGTTQKIGNFEKVKPSDPESYKKAMSDLNSMQEALQFLRSEKSRIHDDKEKAVEFSD
                     IEALEKRVQNAQPKLLTALLDKTTRDISALVKIPRKLTFEDIKSMTTKLNSYLETLEL
                     IRNERIKQHGKSPELLDMSDLDSLKGQLQTFNQNLTGMILNATKDGLDRINDRANFKE
                     GEPYVKACLDLLTELEKTLDSSVKGMKQKEDISACRNSLLDKQEKANSGMLDLQSKSK
                     DLITQLRDICKIHHGNLAEARRTKLHSLDNQEGGLLGGLWSVTNKLGVTTDTVGIERM
                     QIKMKEQALARFKTELNNDKYDTNQVIAFLANKKPSELEEGLGISKENAEELHGLLSK
                     LTSKMASKIEIEENTQLIDEISTKIGTEPVKLELTHTIDEDESDTYRRESGYF"
BASE COUNT         1929 a         1529 c         1461 g         2815 t
ORIGIN      
        1 taaatggtat gcaaactggt cgaaatttcg ttgccagtag atggatatat tctcttttat
       61 tttaaatcca caacaaaaag tcgggtcaat aagacccgac ttcagaattt catacgattt
      121 gggttaaggt caattaaaat cctatagttt ttttattgga ttcatcttct tcaaccatga
      181 caaatttcaa atgctcaggc tctctaccta tcttggttga aatgtcatcg attgctttca
      241 ttctttcttc aattttttct ggagaagtgg ttttagactc caaattggtt aagaccatat
      301 ataaatccat cgcattgtca ggagatattc ccaatccttc ttgcagttca gaaggtcttt
      361 tccttgccaa attagagatt agctgatccg tatcaatctt gtcattgaat aattcagtcc
      421 tgaatcgtgc aagcgattgt tgtttagtcc tgatgtcaag tcgttcatct gtggttaagc
      481 ctactttgtt ggtaaaaccc cataacagat tgcccaccat tcttgttatt ccagcttctc
      541 tgtccagtcg atccagttct cgtaatcttg catttcttgt tttatccaga tcgtcatagt
      601 tagcttgaca tatctcacgt aattgtgtga cgaagacttc actcttgagt tgcagttgta
      661 ccatttgagg ataggctttt tgttttgcaa ccagcaaatc attgagtttg ggaatgtcct
      721 ctctcagttt ctctgatgtc tttgacttat ccaatgtttt ttcgagtttt gcgagtaact
      781 cgaaattctg ttgtatttcc ttttcttgca tgtgaaaagt tttcgcttcc atttgactga
      841 tggaaacccg gatagtattt atcaaagacg aaacaatcga ttgattgatg cgatctaagt
      901 tgccgcttaa tttatccaaa tcagagaggt ccagcggatc tttcgatgaa ccgtgtttct
      961 tgattcgatc attacgcaag gtctcaatat cagcggttct gtcaataaat gctgctattt
     1021 ttgttttcaa ttcagtctga tttttgggtt tttcattttc aagcgctgaa atttgtttgg
     1081 ttacttcaag taaataagca tcggctaatt tggtatccat gaactttatt tgttgttcca
     1141 gtttttcaat atctgaaaaa tcaactttat cagttccaac gtgcatccgt attttttcat
     1201 tacgtaatac ggttacttct tgtttcagat tgctcaagtt agatatgcct ttcttgacag
     1261 catcaagatt gggtaatttc cctgtttcaa gttcagtaat tttcttcgat gtgttttcta
     1321 tcagggcatc atagcgttct cttactttct tttcatctgt tactacaggg gtaacaacag
     1381 cacgctgcaa tgattccagt tcttgcctgg tgctctggat ttgttctctt agctctcttc
     1441 taattgtacc tatctctttt tcagatggta tgtttttgag tgcatccagt gcgccagcga
     1501 tattttgctt ttccagcgct tcctggcatt gagttaatgc gtcgagcact actttatttg
     1561 caaccatttg tttaagggca tccatctgcc ttttggcctg ccttgcttct tcaaaattga
     1621 aggctgacca ttgtccgtcg gatggaaatg cggctaaggc cttacttaaa gcgaccgcat
     1681 cactctccat gaatccatta agacaatttt ccaaaacagg gccaacggta ttttttagaa
     1741 aatttttgta accagtagat aaatgtgaat attgcactgc ttccacttcg ttttgtaaat
     1801 tgaaaatttt ctcaagatca ggtgctacct gggttttttc aatagagctt ttgatttcgg
     1861 ctaacttggc agtttgcatt cttaaaaacg gttcaaccgc atagccgctt tcatgacgtg
     1921 gtataaagtc agggcttttc acagcgacaa aggtaaagac atatcgattc tctccttgtc
     1981 gcgttttacc atccagagtg actttgactg gaaccagggc aacgtcttcc ggcaaataaa
     2041 tggagaattc actttcagtg ccctccccat ggcgtcctac ttgcttggga tgcagcaaac
     2101 catctgggtc aagcatttca aagatgacat tggaatccca ggtttctgtt acaagtttaa
     2161 tatccgtagt cgtactggca ttagttctgc tagatatttt acttaaatca tttaacttga
     2221 tttgtttaaa ggcctctatt gaatgatcgg tgaatagacg ttctgtcgta ttggcaatca
     2281 ttgtgttcgc ctgatctatt aatcccttgg taaactcttc tggcaggttt aatccacgaa
     2341 atagtctttt cggagcagga gcgtgtgacg tatccactaa tgctttgcta gtcaaaggtg
     2401 tcattaaagt agtaatcagc acttccttga aagtagagcc tttatcccgg ttttgtgcaa
     2461 tggcattgac ccggctaaag taccacgcat cattttgcat gaggaaaatt aaatcgtgtt
     2521 tcttttgggt gatgttgtta ctcctattat gttgattttg gtcattgacc caatattctc
     2581 caattttcac cggaacggct tctggattgg tagaatgaaa ctcggtgtct ttttcatgtc
     2641 gcattaaagt atcgatgttt cctatgtcgt tgtcattgag cagtgagcct acaacagcca
     2701 tctcgcattt cgctaacaag tcacggaaga attgttgttc cagtttctgg ccattgatga
     2761 gtacatgatc atcatggatg caatcaggat tggccaagat ttgctgaatg atgcgggctg
     2821 ctgcaatttc atcgacatta ggttgtcttc ttcctttctc tttgctggat tgaaaagctt
     2881 gtttaatagg atcaattttt gccttgtcat gggctgtttg taattgtttc aaacaaaaat
     2941 caacatcgtt ttcgcatcgt gctctgttta aagagttcag tcgattccaa taatccattt
     3001 cattcatacc tggcattcca ccgtgtaaat gtttgccagc accggggaaa ttactttgta
     3061 cttcaggaag ttttaaaaac tcgtccactc gcataaagcg ttcattttct ttcagtttgt
     3121 acgtttggga gaaaaatttt aaatcacctt ccttttgtcc ttttttcgga gcttctcgta
     3181 taggttggcc atcttcaccg atgacataac gacctaaacc agaaacaacc aaatgaggct
     3241 ctttgttatc ggagtcaaaa cctgctacta cttcataggt tgcatgaaaa aactgtcttt
     3301 gtattccaaa aacagcttcc gttgcctggg tacctatcca acgttgcatg gagctgaaat
     3361 aacgctgcaa gaatgattct ggaggttgtt tcacccgaac caaatccacc atatggccct
     3421 ggttaatcag tacatgagct ggggttgaat cccaatcatg acttttatct tcaattactc
     3481 gggcataaaa attgacatct tcctgatctt tgaaaacatc aggaatcaaa gtggattcat
     3541 tgtctttcac atattttaag aaagcatccc ggctttgttc atgatatttt tggtagttct
     3601 tggcatcgga tgcctcatca tcacgtccag ctacaaaaaa tgcctgggca atcatgattt
     3661 ttttcagttc ttcaggggta acgtcagcaa tagtacgtcc gtctttgaat ttccctaatg
     3721 tttcaccccg taatttggct ttacgcgctt cttcaacaat caattcagca taagccattg
     3781 tacgtaaagt atgggaaaga ccatgatgtt gtctgtacac cattcgaccg ccaaaattcg
     3841 cgggtgctga ttcaactggt ccggtatacg gttttccaag atagttgtca tacgcgtatt
     3901 gtgccgcggc tttaacggta tcatcagtat ataatgaaaa cccattccag acaggcacag
     3961 atttatcttg cggtgcagtg ggttttacag gtgtttcttt aggggaggtg tcttttcccg
     4021 gaaggaccag ggtgggaatt tctattttta aatctttttc agtgttttcc cttaatgctt
     4081 ttgcatcaaa ttgctcgtca ccatgaggaa attcatcagc aacagctcgt aaccagccgg
     4141 taatttttcg ataaccgtca tgatcgagct cattacgcat ttcatcggta atggtttgac
     4201 ccagattcaa caaagtagga gggatgggtt gatttaatgc ctggtgtgct cttaaaccgg
     4261 cagaggcgac ccaatagccg cataacccca tattcaaacc agaatcatta ggaataactg
     4321 aaagggtaga gcccattggt aattgtgctg ctaaaatgtt ttgataacct tcacccgagg
     4381 ttttgcctaa tgggtcaaag aggtagtatt ggttaccagg ccctttgatt aacataatcc
     4441 agtgtccgga gtctcggtgt ggtcctaccc cggttaacac gggcatgaaa ccttgcctgt
     4501 tcagggccgc tgtatcaatg gttggtactc cattgtaaat actaccgctg gtaatatcgc
     4561 cataccccat gcgtgcaaaa atagcatgca taccttcttg agttagttct accccttcga
     4621 catacttagg cattatgatt tctcccaaac tgatgttcat aagtgagcaa acgagcacat
     4681 ttatgaatat aaatatttca aattgtatgt atattataga agtgtagtat taagaaagta
     4741 ttaaatgcaa aaaaaatgtc catattgtga atttggtatt taaaaatatt gggatgtgtt
     4801 tctcttgtat aaagagtagg gcagtaaaat aaaggcttga agggatgagt ttaagcagaa
     4861 aagaagtttg tcctgttttt atagtgtttc tttattagga aagatatgct ttaaagcagt
     4921 taacagaatg agaaaataaa aacacaatgt ctttctcttc tcatctgcta gcgccttcga
     4981 tcattcacta aatcatttct ccatctaaaa gtaaccactt tctcggcgat aggtatcact
     5041 ctcatcttca tctatcgtgt gagtcaattc gagttttaca ggttcagtgc ctattttagt
     5101 tgaaatttca tcaattaact gtgtattttc ttcaatttcg attttagaag ccattttact
     5161 agtgagttta cttaacagtc cgtgtaattc ttcggcattt tctttcgata tgcctaaacc
     5221 ttcttccagc tctgatggtt ttttatttgc taaaaaggca atgacttgat tcgtatcgta
     5281 tttgtcatta ttcaattctg ttttaaatct tgccagtgct tgctccttca tttttatttg
     5341 cattctctcg atacctactg tatctgtggt aacacctaat ttgttagtaa cagaccataa
     5401 tccacctaac aatcctcctt cctgattatc aagtgagtga agcttggtcc ttcttgcttc
     5461 agctaaatta ccgtgatgaa ttttacagat atcacgtaat tgggtaatca gatctttact
     5521 cttggattgc aaatctagca ttccggaatt ggctttttcc tgtttatcaa ggagagaatt
     5581 tctacaggcg ctaatgtctt ctttttgttt cattcctttt accgaactat ccagtgtttt
     5641 ttcaagttct gttaaaagat caagacatgc ttttacgtaa ggctctcctt ccttaaagtt
     5701 agccctgtca ttgattctat ccagaccatc tttagtagca tttagtatca tgccagtcaa
     5761 attttgatta aaggtttgca attgaccttt taaactatcc aaatcggaca tatctagaag
     5821 ttcaggggat ttaccgtgtt gtttgattct ttcattacga atcaattcca gggtttccag
     5881 gtaactgtta agcttggttg tcattgattt tatatcttca aaggtcaatt tcctgggtat
     5941 ttttactaat gctgagatgt ctctggttgt tttatccagg agtgctgtca gtaatttagg
     6001 ttgagcattt tgtactcgtt tttccaaggc ttctatatca gagaattcca ctgctttttc
     6061 tttatcatca tgtattcggg atttttcact gcgtaggaat tgtaatgctt cttgcatgct
     6121 atttaagtcg gacatagcct ttttataact ttcaggatct gaaggtttga ccttttcaaa
     6181 gttaccgatt ttttgtgttg ttccatatag aagaaattga tagtattctc tcgcttttgt
     6241 tgcggcaaca tgttctagcg agacgggttt actacctatt ttggttgata tttcatcaat
     6301 taattttgtg ttttcctgca atttatctat tggagtagct tttgcgtcta gttgctttaa
     6361 caatccatgt aattgctcag cattctcttt cgatatgccc aaattttctt ccaattcgga
     6421 tggttttttc tttaccagaa attgaataag ttgactcaca tcatttttgt cattatttaa
     6481 ttcagtttta aaccttgcaa gttcatttgc tttttttacc tcagcttggc ttatagcagt
     6541 cgttatctga ctaaagaaac cgtctaatat tcctccggtc ttgttcagta gctgttgttg
     6601 ttcctctctc gttttggcca aattatcatg atgaatattg catagattgc gtaattgtgt
     6661 aatcagtgct tcactcttgt attgcaattg cagcatttct ggataagctt tctcctgttt
     6721 ctccgttaga gagtttttat aggtgttaaa gtcttctttt tgtttgattg ctttttctga
     6781 accatccaga gttttttcaa gtccagcaaa atggtcaagg cagcgtttta tatattgtcc
     6841 ttctttttct agtgtagccg gatccttgat tttatcgagc gagtttttag ctccatttaa
     6901 taaaatttgg gctaaatgct gactcacgcc ttgtagttga ttttttaatt tatccaaatc
     6961 agacatatcc agcggctcag tggattcacc atgctttttg actctttcat tacgaatgaa
     7021 ttccaaggtt tccaggtaac cattaatctt ggatgtcatt gatttaatat cgtcaaaact
     7081 gattgacttg ggtatttttt caagctcttg aatacctttt attgttcctt cctcaagtga
     7141 gggatataaa ttgggttgtg cttcttgtaa tctcttttcc aaagcttcta tgtcagaaaa
     7201 gtccactgct ttgtctttat cagtatgcat gcgaattttt tcattgcgca gaagggttaa
     7261 ttcttgttgc atgttattta aatcggcaat ggctttgttg taactgtcaa gattggctgg
     7321 tttgattttt tcaaagtcgg tgactcgttt tgtcacgtct gtgacaaggg tttcataccg
     7381 tactttcatt ttttcagcat cttgtacgat tggcgcagtg gttgcacgtt gcatggattc
     7441 aagatttcca gttagctctt gcttgacacc aacgatttgc cctcttaact cttgtcctat
     7501 tgtcttgacc ttacctattt ccttttctgc aggcagttta ttgagcgctt gtaatgcttc
     7561 tgtgacattt tgtttatcca gcgcttcact acattcggtg agggctggga taatttggct
     7621 ttgcagtatg attttctttt caatctgttg ctttaataca tccatttgag ctttggctct
     7681 gactgcttct cctgatttaa aagcagacca ttgcttatcg gtagggaatt ttgc
//
LOCUS       DASLOE010000144         7723 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.145, whole genome shotgun sequence.
ACCESSION   DASLOE010000144 DASLOE010000000
VERSION     DASLOE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.145"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            54..539
                     /locus_tag="U6309_002344"
     CDS             54..539
                     /locus_tag="U6309_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN5505777.1"
                     /translation="MEKVNLTKQFAYRLRDAMIAAGHNSQRSPSGVCIHKLAEMTGYS
                     LQICRKYLRGEVIPDPVKLIEIASKLNVSPGWLLFGETHSDPGTSKEHITISKSLLHH
                     IFTKAACLYNRELVDDDVSDFLMDLINDISLINATEQQSKKIIDLAFSSVSHFRHLEG
                     T"
     gene            complement(891..2453)
                     /gene="wipA"
                     /locus_tag="U6309_002345"
     CDS             complement(891..2453)
                     /gene="wipA"
                     /locus_tag="U6309_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector WipA"
                     /protein_id="HEN5505778.1"
                     /translation="MPKRLINKNIDIYNYPNELEDNLGSISLGDLHGNAIKLIHFLFR
                     HKIIKFKPEISNFHEAYQQFVTIYEQYDDMVQEYLEIRTLLQLIQIKITNAQQRILDI
                     EQKLSLAIDHQKEFSQSLLQLKKPIEANLQMAEKSKAGLEEKLSGLKTRLPSCIERFN
                     KFMTQIEINDIKTLIRLLGDEVADRGSCDYFTLRILDFLYQNQIAIKIILSNHGYEFI
                     HAYEKLVIGQPFKPKGYIGDIQIKSFWGIQLLLEQSAITQEELRSLVERAYKPTLKII
                     DYSLCEDGITLYSHAPIRFDSIRMAASQLGVAYNDSTKEALAETIDQLNAQLQIYMKN
                     NMLHLLFENNEINDPTNMTDEERNASPLIYLVWNRWNESKEVENARPGKYNGYFVTYV
                     HGHDPFQSPLTYVYNLDTMCGKYSRVDEEEQINKAFQFLTENRHTNVDKTASESLLRN
                     ISRYKVLDSDEYTLKHKIPKTSPELATDILDCKIKESLIKLSLLGKPKAVSGSLSVDQ
                     NTTILNQAFLGK"
     gene            2734..3885
                     /locus_tag="U6309_002346"
     CDS             2734..3885
                     /locus_tag="U6309_002346"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEN5505779.1"
                     /translation="MRLLSFKKSKIVSIGTELEFQIIDCSSLSLVSRSKELMRALKDM
                     RYRDQIKPEITQSMIEINSSIHQSAKEMYDELLELQKILVETAASIDIAFCGGGTHPF
                     QQWTMQKIFPSKRFKKKFNQYRYLSKRATVFGQHIHIGCPTGDDAIYLTHALARYVPH
                     FIAISASSPFYLGINTNYCSSRSTIFNAFPLSGVIPYLRNWQEFSDYYRKMYRWKIIE
                     NMKDFYWDIRPKPELGTIEIRVCDTPLTLRKSILITAYIQALALYLLEEKPVQLSHDL
                     YYVYNYNRFQASRHGLEGELTVTDKDRPIPIMDDILETIKKIEQYINGLGNSEYIEEL
                     CSDVINKQNDSVLINKIYKQDGSFSKLVAAQCELWLTDSKDRKWMAQPS"
     gene            3864..4895
                     /gene="legN"
                     /locus_tag="U6309_002347"
     CDS             3864..4895
                     /gene="legN"
                     /locus_tag="U6309_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector LegN"
                     /protein_id="HEN5505780.1"
                     /translation="MDGTTFLSTKEKILEIKPLMESAFGNILGYQELENMLAICTLVP
                     FSNNQILFHQGDRIKGIYIIIKGTVDLIARTLGKGITKIETCLAGDFLGETSFLLNEP
                     SATSAVAKDNVTCLFIPSAYFELMSAFSPEAKYKIMKAIGYQACGRLKQLHDKIIDFI
                     SHSDMQSLSFLGRVIQTFTQPKTHTLDESGVDKDQIYKKTLFHSFTQDETDELLEHAI
                     PLTANKNCVLIHENETIPVCYIVIYGAVQSNVIQNSKIAKLSVIGPDSLFASTACFLS
                     DTKFSITFITCESATLLKMSETELQYFQKNKPMIWYKLFNLICGSIISLARSLKKLDV
                     RLNIEKYNQ"
     gene            4902..5819
                     /locus_tag="U6309_002348"
     CDS             4902..5819
                     /locus_tag="U6309_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEN5505781.1"
                     /translation="MCRILSYLGQPTVVEELLYKPDNSFIKQSYHPKYLPYLLNLAGF
                     GMAAWDRNSINPLQAYVYKTSKLPFYDENLHNLAEKIAPHCLLAHLRGVSYHDKQVVT
                     DQNVHPFLYPVTSIALAHNGMLHNFETMRYDLLKYVKTEYRKYIKGTTDSEWIYAVFL
                     SQLENPKGHFDASEIINAVIATLKILKTVRQQNNIVINSPVNLFLTNGDFIAATRFCL
                     DYGWKPKDAPQSTHFTYHSLWYTYGERYGFYENEYKMKGGTINSSIIIASEPLTENTT
                     TWLEVPEYTLIFANQIDGEIKITSQDINI"
     gene            complement(5947..7002)
                     /locus_tag="U6309_002349"
     CDS             complement(5947..7002)
                     /locus_tag="U6309_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEN5505782.1"
                     /translation="MVNSDLILSPYQLNEKITLKNRVIMAPMTRNMANDDFSPTRQMA
                     DYYARRADAGLIITEGTIISPDGQGYSNAPGIFTQTHIDGWKMVTEAVHERGGKIFLQ
                     IWHVGRVSHPIFLHGNLPISASETMMTGKVRRADDLYYGKSRAATLEEINELIQNYAL
                     AARRAMIAGFDGIEIHGANGYLIDQFLHYHTNQRHDQYGQHPENMGRFALEVVKACIN
                     EIGGQSVGLRLSPGAYLNEIVGDERDAMVFRYVLEELNHYGIAYVHTGNFDDGIQFKE
                     LGFKTMTEFMRAHFKGTIIASGSYTLHQARKDIQNKKYDLIALGRPFIANPDLMNRIK
                     WNADFIPYENRMLDTLF"
     gene            complement(7074..7373)
                     /locus_tag="U6309_002350"
     CDS             complement(7074..7373)
                     /locus_tag="U6309_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN5505783.1"
                     /translation="MNVINILKALSNEKRLQIIRWLKEPDKHFSSSHCDVSKDGVCVG
                     LIEKKSGLSQSTVSQYLSQLQQAGLIITERRGQWTYCKANANLINEFIKELKVMI"
BASE COUNT         2464 a         1511 c         1246 g         2502 t
ORIGIN      
        1 accctttgct acacttttac cactttttaa tatcttaaaa ttattactcc aatatggaaa
       61 aagttaattt gacaaaacag tttgcttacc gcttgcgtga tgccatgatt gcagcagggc
      121 ataattctca acgctctcca tcaggagtat gtattcataa attagctgaa atgacaggtt
      181 actctttgca aatatgcaga aaataccttc gtggagaggt cattcccgat ccagtcaagc
      241 tgatagaaat tgcatcgaaa ctaaatgttt cgccaggatg gcttttgttt ggtgaaaccc
      301 atagtgatcc tggaacatct aaggaacaca tcacgataag caaaagttta ctccatcata
      361 tttttacaaa agcagcttgt ctatataatc gtgagcttgt ggatgatgat gtgtctgatt
      421 ttttgatgga tttgatcaat gacattagtc tcatcaatgc caccgaacaa cagtccaaaa
      481 aaataattga tttggctttc tcttcagtga gccattttag acacttggaa ggaacgtaga
      541 gtaagaaact ttcatggaaa ataacccaaa attatgtgat tcattcaata gcaagccaca
      601 aatcttgtgc ttttactcgt gagttatttt ctactcatca agaagatttt taaaaaatag
      661 ggccatattc ctccaatttg ctgcatccat tttttagtta ttatgaagat tttccttctc
      721 aatatttaat tttaaactca caatgaacaa taaacgtcca tcccgacttt gtaatgaatt
      781 acttgatttt taaagaactc gataatctac taacgtacct caattttttc gaagcatttt
      841 gtgcgcattt tatggctatc tctaaaacgc agcgcttcat agagagtcgg tcatttgccc
      901 agaaacgcct gattaagaat agtagtattc tggtctacac tcagcgaacc tgatactgct
      961 tttggtttcc cgagcaagct cagttttatt aaactctcct taattttaca atcgagaata
     1021 tctgttgcca attcaggtga cgtcttaggg attttgtgtt ttaaagtgta ttcatccgaa
     1081 tccagcactt tataccttga tatattgcga agaaggcttt cacttgctgt tttatccaca
     1141 ttggtgtgtc gattttcagt caaaaactgg aaggccttgt taatttgctc ttcctcatcc
     1201 actcttgagt attttccaca catagtatcc agattatata cataagttaa tggactttga
     1261 aaaggatcat gtccatgaac atacgtgaca aaataaccat tgtattttcc tggtcgagca
     1321 ttctctactt ctttgctttc attccagcga ttccatacta aataaattaa cggcgatgcg
     1381 tttctctctt catcagtcat attggttgga tcgttaattt cattattctc aaataacaaa
     1441 tgcagcatgt tgttcttcat ataaatttgc aattgggcat taagttggtc tatggtttca
     1501 gccaatgctt ctttggtgga atcattataa gcgacaccca actgagaagc tgccattcga
     1561 atgctatcaa agcgtatggg agcatggcta tacaaggtaa taccgtcctc gcatagagaa
     1621 taatcgatta tttttaaagt tggtttataa gctctctcga ctaaacttct caattcctcc
     1681 tgggtaatgg cactttgctc cagtaataat tggatacccc agaatgattt aatttgtata
     1741 tccccaatat aacctttggg tttaaatggt tgaccgatta caagtttttc ataagcatga
     1801 ataaactcgt acccatgatt ggataaaatg atcttgattg ctatctgatt ttgataaagg
     1861 aagtccagta ttcttaaagt gaaataatca caactccccc gatcggccac ctcatcaccc
     1921 agcaatcgaa taagcgtttt aatatcatta atttctatct gcgtcatgaa cttattaaat
     1981 cgctcaatac atgatggcag ccttgttttc aacccactta atttttcttc caggccagct
     2041 ttactctttt cagccatttg taaattggcc tctattggct tcttaagttg caataatgat
     2101 tgagaaaact ctttctgatg gtctatggca agtgataatt tttgctcaat gtctaaaatt
     2161 ctttgctgtg cgttagttat ttttatttgt atcaattgaa gaagtgtacg aatttccaaa
     2221 tattcttgaa ccatatcgtc atattgttca taaattgtta caaattgctg gtaagcttcg
     2281 tggaaattgc taatctctgg tttaaattta attattttat gacggaaaag aaaatggata
     2341 agcttgattg cattgccatg caagtcgccc agtgaaatag aacctaaatt atcttccaat
     2401 tcattaggat aattgtatat atcgatattc ttgttaatga gacgttttgg cattccctag
     2461 tccatgtaaa aaactcccct tcaaaataag ggcatactca aagtcgacaa taacaaatat
     2521 tcacttttca tgtaataaca tggaagtatt aaattcatgc aatgaactcc cactttaatt
     2581 gccctggaat agcagcaaac aagctaaatt attaaacaat ttttatgggt ttggggaaac
     2641 gtgaaccttc atatctattc ccattaaaca atcatctaac caaataccct atctgctata
     2701 cttaaacaag gaaaataata gtcagagaaa ggaatgagat tattatcttt taaaaaatcc
     2761 aagattgttt caattggaac tgaattggag tttcagatta ttgattgcag ttcattgtct
     2821 ctggtttctc gttcaaaaga attgatgaga gccttaaaag acatgcgcta tcgcgaccaa
     2881 attaagccgg aaattacgca aagcatgatt gaaatcaatt catcaattca tcaatccgct
     2941 aaagaaatgt atgacgaact gttagagtta caaaaaattt tagtagaaac tgctgcaagc
     3001 attgatattg ctttttgcgg cggagggaca cacccatttc aacaatggac catgcaaaaa
     3061 atttttccat cgaaacgatt caaaaaaaaa tttaaccaat accgttatct cagcaagcgt
     3121 gctactgttt ttggacaaca tattcatatc ggctgcccaa caggagacga tgctatttat
     3181 ttaactcatg ccttggcaag atatgtcccc cactttattg caatcagcgc ttcatcgcct
     3241 ttctatctgg gtattaatac aaattattgc tcatcacgct cgacaatttt taatgctttt
     3301 ccattaagtg gtgtcattcc ctatctgaga aattggcaag aattctctga ttattatcgc
     3361 aaaatgtatc gttggaaaat cattgaaaat atgaaagact tttattggga cattagacca
     3421 aaaccagaac ttggtacaat agaaataaga gtctgcgaca ctcctttaac tcttagaaaa
     3481 tcgattctta ttactgctta tattcaagca ttggcactct atctcctcga agaaaaacct
     3541 gtccagctgt ctcatgattt atactatgtc tataattaca acaggttcca agccagcaga
     3601 catggtctgg aaggagaatt aactgtcact gataaagaca ggcccatacc tatcatggat
     3661 gacattcttg aaacaataaa aaaaattgaa caatacatta atggacttgg aaacagcgaa
     3721 tatattgagg aactgtgtag tgacgtcatc aataaacaaa atgatagcgt tctcataaat
     3781 aaaatttata aacaagatgg ctcatttagt aagctggtag cagcgcagtg cgaactatgg
     3841 ttaactgatt caaaggatag gaaatggatg gcacaacctt cttaagtacg aaagaaaaaa
     3901 tcctggaaat caaaccatta atggaaagtg ctttcggcaa tattctagga tatcaagaac
     3961 tggagaatat gttggcaatc tgtacacttg taccattctc caacaatcaa atcttgtttc
     4021 atcaagggga tagaataaaa ggcatttata tcattataaa aggaactgtt gatctgattg
     4081 ccagaacctt aggcaaagga ataaccaaga tagaaacttg ccttgccggt gattttttgg
     4141 gagaaacttc ctttctcctc aatgagccca gtgccacatc agcggttgct aaggacaatg
     4201 tgacatgctt atttatccct tctgcctatt ttgaactgat gtcggcattt tcacctgagg
     4261 ccaaatacaa aatcatgaaa gcaattggtt atcaggcctg tggtcgttta aaacaattac
     4321 acgacaaaat cattgatttt atttctcatt ctgatatgca aagcttatct tttttggggc
     4381 gagtcattca aactttcacc caacctaaaa cacataccct ggatgagagt ggagttgata
     4441 aagaccaaat ttataaaaag actttatttc attcttttac tcaagatgaa accgatgaat
     4501 tgcttgaaca cgctatacca ctaactgcca ataaaaattg cgtattgatt cacgagaatg
     4561 aaaccatacc ggtatgttat atagtgatct atggggcagt tcagtccaat gtcatacaaa
     4621 acagtaaaat tgccaaattg tctgttattg gtcctgactc gttgtttgcc agcacggcct
     4681 gttttctgag tgacactaaa ttttcaatta catttattac ctgcgaatca gcaactttat
     4741 taaaaatgtc tgaaaccgaa cttcaatatt tccaaaaaaa taaacccatg atctggtaca
     4801 aattatttaa tttgatatgt ggatctatca tttccctagc tcgttcctta aaaaaattgg
     4861 acgtgcgact gaacatagaa aagtataatc agtgaggtgc tatgtgcaga atattgtctt
     4921 accttgggca acccacagtg gttgaagagt tactttataa acccgataac tcctttatta
     4981 aacagagtta tcatccaaaa tatttgcctt atttattaaa tctggcgggt tttggtatgg
     5041 ctgcttggga tagaaattca attaacccct tacaagctta tgtctataaa acatccaaac
     5101 ttccttttta cgatgaaaat ttacataatt tagcagaaaa aatagcacct cactgtcttc
     5161 tcgcccatct acgaggggtt tcctatcatg ataaacaagt agttactgat caaaacgtgc
     5221 atccatttct ctatccagta accagtattg ctttagcgca taatggtatg ctccataact
     5281 ttgaaaccat gcgctatgat ctgctgaaat atgttaaaac ggaatacaga aaatacatta
     5341 aaggaactac agatagtgag tggatctatg ctgtttttct ttctcaatta gaaaatccta
     5401 agggtcattt cgatgcctct gaaatcataa atgctgtgat tgccacttta aaaattttaa
     5461 agacggtacg ccaacaaaac aacatagtca ttaactcacc agttaatctt tttttaacca
     5521 atggtgattt tattgccgcg actcgatttt gtttggatta cggctggaaa cccaaagacg
     5581 ctcctcaatc gacacatttc acctaccatt cactatggta cacttatgga gaacgatatg
     5641 gattttatga aaatgaatat aaaatgaaag ggggaaccat taattcaagc attattattg
     5701 cgtctgagcc attaactgaa aacacaacaa cctggcttga ggtccccgaa tatactttga
     5761 tttttgcgaa tcaaattgac ggcgaaatca aaattacttc ccaagatatt aacatttgaa
     5821 atgctctaca tttagcatcc caaaacactt tctcatcatc tccttgtacc ttcttcatca
     5881 cttcattcca aaattttctg gtaatacaga aagcaagaaa ggtaattgaa taccccgctc
     5941 aagtgcctaa aacaaggtat ccaacattct gttttcataa ggtataaaat cggcattcca
     6001 cttgatacga ttcattaaat caggattagc aataaatggc cttcccaaag caattaaatc
     6061 gtatttttta ttttgaatat cttttcttgc ctggtgaagc gtgtagctac cgctggcaat
     6121 tatagttcct ttaaaatgag ctctcataaa ttctgtcatc gttttgaacc ctaattcttt
     6181 gaattgaata ccgtcatcaa aattacctgt atgcacataa gctattccat agtgattcaa
     6241 ttcttccaat acatatcgaa ataccatggc atctcgctca tctccgacaa tttcattaag
     6301 ataagctccg ggagaaagac gcaatcctac agattgccca cctatttcat tgatacaggc
     6361 ttttaccact tccagcgcaa atcgccccat attctctgga tgctgtccat actgatcatg
     6421 acgttgattg gtatggtaat gtaaaaactg atcaatcaaa taaccattgg caccatgaat
     6481 ctcaatgcca tcaaacccgg ctatcatcgc tcttcttgca gccaacgcat agttttggat
     6541 taactcatta atttcctcta atgtagccgc ccttgatttt ccataataaa gatcatcggc
     6601 acgccggacc ttacctgtca tcatcgtttc tgaagcagaa attggtaagt taccatgtaa
     6661 aaaaatcgga tgagataccc tgccaacatg ccaaatttgc aaaaatattt tccctccccg
     6721 ttcatgcaca gcttcagtta ccattttcca accgtcaata tgggtttgcg taaatattcc
     6781 tggagcatta ctataacctt gaccatcagg gctaataata gtgccttctg taataatcaa
     6841 cccggcatca gcccttcgag cataataatc tgccatctgc ctggttggac tgaaatcatc
     6901 gttagccata ttacgagtca tgggagccat aatgactctg ttctttagtg taattttctc
     6961 attaagctga taaggactta aaattaaatc ggagttaacc atgataaatc tcctgcatgt
     7021 cataacaata tattggaata tcgcaaatat tcgatttatt aaagcaaaat tttttaaatc
     7081 ataactttca attcttttat aaattcatta attaggtttg cattcgcttt acaataggtc
     7141 cactgtcctc tacgctcagt gattatcaaa ccagcttgct gtaattgtga taaatattga
     7201 gataccgtgg attgagataa gcctgatttt ttttctatta agccaacaca aacaccatct
     7261 ttgctcacat cacagtgcga cgaactgaaa tgtttatctg gctcttttag ccatctgatt
     7321 atctgtaatc gcttttcatt agaaagcgct tttaatatat ttattacgtt catattttaa
     7381 ttatatcgtt ttattgcgat atgtcaattt agtgattacc catagtcatg tgtgttgaaa
     7441 tttcaaataa cctgaccgat acaaatacta aaaataagac gaatgggaga gataaatgct
     7501 caatatggat attttgaata ttaaccctga aacagttcct gcgacaaata gtattgcaaa
     7561 agagcttgtt gatttaacta ttttacactc tcaaaattta ttttaagagc ctttaaaatc
     7621 tattttcatt caggcaattt atcaaaactt ttcatgacgg attcattaaa ctctttcatt
     7681 acatcacttt cataaaaatc ttttttaaat tcttcatcct gaa
//
LOCUS       DASLOE010000145         7716 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.146, whole genome shotgun sequence.
ACCESSION   DASLOE010000145 DASLOE010000000
VERSION     DASLOE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.146"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            252..473
                     /locus_tag="U6309_002351"
                     /pseudo
     CDS             252..473
                     /locus_tag="U6309_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            565..855
                     /locus_tag="U6309_002352"
     CDS             565..855
                     /locus_tag="U6309_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN5505784.1"
                     /translation="MDKKLFERLYESMTQMNEIIDGERVPSREFVVDAVHVKEIRKKT
                     GLTQEKFCHLIDVNIGTLRNWEQGRREPTGPAKALLRAINKDPEHVLAALAG"
     gene            complement(1147..1338)
                     /locus_tag="U6309_002353"
     CDS             complement(1147..1338)
                     /locus_tag="U6309_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505785.1"
                     /translation="MRNKDEKAKEIATDSRCAEANRLTHLITGAILADNCFFPSGAYS
                     TKPMVSNSQPPREKTTPSM"
     gene            1605..2498
                     /locus_tag="U6309_002354"
     CDS             1605..2498
                     /locus_tag="U6309_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505786.1"
                     /translation="MKDKTIPSPDAKSFNKEEFSENVAKGAIVGATSTTMTFGLFDKP
                     LAIATKASSEVAQHPWRGIKNLFAGALNGSFSTSWNNSLYAQAYRAYLEHPVKGYPVA
                     LLNSCLKNVVFFPAKYGSERAFYALSGNEQQAKNYSGFLAGLMTVYLTAPVSVVKARL
                     MTNVPLNTLSLKRLGSGVNAIAMRDCIQYGIYFNAKEWLNEKISGGVMAGGIAGIVGY
                     TFSNPLSVIGLNQKTAVESVNIPTMAKRIYQANGVKGFYPLVGLAALGMFARGVAIHQ
                     GTKLFESLQENEACGSPTRNR"
     gene            2738..4180
                     /locus_tag="U6309_002355"
     CDS             2738..4180
                     /locus_tag="U6309_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN5505787.1"
                     /translation="MKISTNELIAVVSQFNPWWRGESIQDLPTWHRAAYQELELWLIK
                     PPVPRAIFLSGARQIGKTTLLLQAIDKLLKEGVKPGNILYVTFDHPLIKMSDIDAVLE
                     AWREREPKTGDLEYIFIDEAQFMRSWGTWIKHQVDFSKSRRIFFTGSATPIIEAEQES
                     GVGRWHTIRLTILSFYEYLQIKKIDLPQLPTVKTLRELFEWPQQEFYKVKELAVHYVG
                     HFHEYLLRGGFPQTAQVENITQAQRLLREDIIDKVLKRDMTALFGVRRILELEQTFLY
                     LCMHDGGLLDMTDLCSNLEVKRPTIQNYIFLLEATNLIYRLPPFGYGKDILRAKYKIY
                     LADAAIAPAVLLKDKSILDDPESLGIATETAVFKHLFARYYSQNVRFSYWRGTKDHEV
                     DVVAQIGDQIIPFEVKYRSQHTNAKALKGLLELCKKKSIAHGYVVTKSLDDFGIMKAL
                     PEIKTNVMQVPAPLLCYWMGESELRQTFGV"
     gene            complement(4342..5829)
                     /locus_tag="U6309_002356"
     CDS             complement(4342..5829)
                     /locus_tag="U6309_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2912 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505788.1"
                     /translation="MSYSKIDVLERKWSSLEAIVKSYTDPVEYHLPEIKKLMTAEFVV
                     DFSSHAIYLHTEYVHRFTQQLINLRNASPNDERLVCIHQLWTDLLNCRNQNPLKIKHH
                     KKLDGECTFNFSLKMMEGKPYGFKEVQILDPNNTAELNKAMGEIKRIDSLAHGANFGD
                     LPFIGDIVLSSMLHYKETICFIAKDKNDEVIGYCWGMMLRDIPAGEKNKVNVFWVMNL
                     AKHPDFYDPNIKVGEALRQHLANTLSKNPDCHFLGYEHVLNHNFHLAIVDGGIPKKEE
                     TISIGQNQYQAKSLVKYEPLKMQYDRKHIIKTNNHQYPYPKMDVIKSKFFTALWYAAP
                     TACDFILGGILIYGRAYYQQWTHSMLGQPVTHRIEKPIPHEQQICDMNILKRIILSDV
                     WGQQGTTLFFDACIPDTIQKLQQLVKEDQIEFASLQQCVAKSGWALCRSKLTAYFYFA
                     ITQSQYPSAAINLLIQNDITPKKWIELISEERQISAQQSSERASI"
     gene            6075..6248
                     /locus_tag="U6309_002357"
     CDS             6075..6248
                     /locus_tag="U6309_002357"
                     /inference="COORDINATES: protein motif:HMM:NF036995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505789.1"
                     /translation="MRIQSTDILHCQFGRGYGKINLTRMLKLSRIFPNREIVSTLSKQ
                     LSWSHFVMVWVLL"
     gene            6335..7606
                     /locus_tag="U6309_002358"
     CDS             6335..7606
                     /locus_tag="U6309_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEN5505790.1"
                     /translation="MSIFRSVLITLLFISSAMAATPDQVTYLPGWGPIKNNQYAGYFP
                     VNPKAGLFYWFVESSNPSMDAPIVLWLNGGPGAASLYGFFMENGPYQVDKNGKLTARK
                     DSWTKAANYLVIDQPVGVGYSYGSSKSYGSEGEAIDQLQGALQLIFKKHPELYGKPLF
                     LAGESYAGKYLPQLAIRLLKDKNMNLKGLLLGDPWINPRLQQKANIDYAYYHGLIDSK
                     ARKRVQILYENCVKEIDKQSPSTSKANKTCEQIQEFIKHESGGLNLANIATGVEPEDT
                     NMVNYLNQKVVREALHIPVTVSEFKTFSTSAAKKLEIGEQDSVADLYPQLLAAGIRIL
                     IYNGLEDGKDSNFLSTELLLASLDWHGKNAFAKAPTCIWRTDNEVSGYAKGAVGLTQV
                     KIRGAGHLAPIDQPARVFDLFKHFINDKPLC"
BASE COUNT         2412 a         1343 c         1562 g         2399 t
ORIGIN      
        1 ccctatctga ttgcgaagga gtttattttg tttttgttaa aattttaatc taacgttctt
       61 ttgccctgat gccattaaaa ataatatatt aagtgcttat tcactaaatt tatgttcgat
      121 attctatctg ccagcaataa agtggcaaag caaacaaaac cctcgtttta tatacatgcc
      181 aaaaatacgt cattgactta cttttattta ggatatatac tttaattcat caaagaaaaa
      241 taagaatagc aatgattttt attgaaactc caatttttca gaagacgtga aggaattatt
      301 atctgatgag gagtatgctg agtttcaaga gtatcttgct gataatcctt tagctggaga
      361 cgtaattcag caaaccggcg gactgcgaaa agttcgatgg tcatttcaag gcaaagggaa
      421 gcgaggtggt gttagaatta tttattatta tgtcactcct gcttcacaaa taagactctt
      481 acttatttat aaaaaaggta ttcaagatga tctgactaca gatcaaaaga aaatgttacg
      541 gcaattaaat gaacggtggt aatcatggat aaaaaattat ttgaacgatt atacgaaagt
      601 atgactcaga tgaacgaaat tattgatggt gagcgtgttc cttcacgtga gttcgtagtg
      661 gatgctgttc atgtaaaaga aattagaaaa aaaacaggcc taacccaaga aaaattttgt
      721 catttaattg atgtaaatat tggaacatta agaaattggg agcaggggag aagagagcca
      781 acaggtcctg caaaagctct tcttcgcgca ataaataaag atcctgaaca tgtattagca
      841 gcattagctg gttgataatt gatgttttaa ctttctataa aaaatgcaga cagtgtcggc
      901 acaattcaaa gtttctctaa aggagctttt aactcaaaat atggaaaacg agtttagcaa
      961 gcgggtatat ggccgataaa atttgccagg gtcaataaat agtattctga tggttagata
     1021 ataaatgatg aagagttctt ttggtgaaat gaataaaaga accctttttt attgggcgac
     1081 tcttaaaaaa gccatttgct ttattctgtg cttttggcaa gtggcatgat ctcattcagg
     1141 ctttgtttac atgctaggtg tagttttttc ccgaggcggt tgtgagtttg ataccatagg
     1201 ttttgttgaa taggcgccgc ttgggaaaaa acaattgtca gcaaggattg cccctgtaat
     1261 caggtgggta agccgattgg cttcggcaca tctcgaatca gtggcaatct ctttcgcttt
     1321 ttcatcttta tttcgcataa caatcctgtg aagttaatct ttgcagagga cactatgatg
     1381 gtctcatgtc atacaaacta agcaaccggg taccgagttg agtccaaagc tcgatcttga
     1441 ttccttcgct aacgcttgca acaaggagta tttcatttgt agtccgttgc gttggtgcaa
     1501 agcaagttaa gttaaaataa tcgaccagtt aatgtctggt taaggatagc attatatcat
     1561 taccgatcaa tcgcgcacgt cgtatgattt aagagtaata agtaatgaaa gacaaaacaa
     1621 tacctagccc ggatgcaaaa tcatttaata aggaagagtt ctctgaaaac gtggcaaaag
     1681 gagccattgt tggcgcaacc agcacgacca tgacgtttgg tttattcgat aagcccttgg
     1741 ccattgctac gaaagcctcc tcggaagtgg ctcagcatcc atggcgtggt atcaaaaatt
     1801 tgtttgccgg ggcactgaac ggaagttttt cgaccagctg gaataatagc ctgtatgcac
     1861 aggcttatcg agcttattta gagcatcctg ttaaaggtta tcctgttgct cttttaaact
     1921 cctgcctaaa gaatgtggtg ttttttcccg caaaatatgg gagtgaaagg gcgttttatg
     1981 cgctttcagg gaatgaacag caagcaaaaa attattccgg attcctggcc gggctcatga
     2041 ccgtttacct cactgcacct gtttctgttg tgaaagcaag actgatgact aacgtacctc
     2101 ttaatacgct cagtttaaaa cgattgggct caggcgtgaa tgccatcgcg atgcgtgact
     2161 gcattcaata tggaatttat tttaatgcca aagagtggtt aaatgaaaaa attagtggcg
     2221 gtgttatggc gggtggcata gctggcattg tggggtatac ctttagtaat cccttaagtg
     2281 taattggact taatcaaaaa acggctgttg aatcagtaaa cattcccacc atggcgaaac
     2341 gaatttatca ggcgaatggt gtgaaagggt tttatccttt agtcggcttg gccgctttgg
     2401 gcatgtttgc tcgtggcgtg gctatccatc agggcactaa actattcgaa tccttacagg
     2461 aaaacgaagc ttgtggaagt ccaacaagga acaggtagta aattcataac gtcccctgtt
     2521 tccatgaaaa tatttcaggg gggcaggagg atgaatcgta ttggacaata aggctataac
     2581 gcggtgacgg tttaataata gtctccgtat tgactcaatt acgagcaagc gcaggtcggg
     2641 ccaaggcccg atcttgctta aatttctaaa aaacacacta ttattcctaa ttagttatag
     2701 tatactttaa ctaaaaaatc attttagtgt ttttttcatg aaaatttcta ctaatgaatt
     2761 aattgcagta gtatcccaat ttaatccctg gtggagaggt gaatctattc aggatttacc
     2821 tacttggcat agagcagctt accaagagtt ggaattatgg ttaattaaac cacctgtacc
     2881 tagagcaata tttttgtctg gagctcgaca gataggcaaa acaacccttt tgttacaagc
     2941 tattgataag cttttgaaag aaggagtaaa gccaggaaat attttgtatg taacctttga
     3001 tcatccatta ataaaaatgt ctgatattga tgctgtatta gaggcatgga gggaacgaga
     3061 acctaaaaca ggtgatttag aatatatttt tattgatgaa gctcaattca tgcggagttg
     3121 gggaacatgg attaaacacc aagtcgattt ttcaaagagt agaagaattt tttttacagg
     3181 atctgcaaca ccaattatag aagcagagca agagtcagga gtagggcgtt ggcatactat
     3241 tcgtctaaca atactctcat tttatgaata tttacaaata aagaagattg atttgcctca
     3301 gttaccaact gttaagacat tgcgagaatt attcgagtgg ccacaacaag aattttataa
     3361 ggttaaagaa ttagcggttc attatgtagg acattttcat gagtatttat tacgaggtgg
     3421 atttccacaa acagctcaag ttgaaaatat tacccaggca cagcgccttt taagagaaga
     3481 tataattgat aaagtactaa aaagagatat gacagcacta tttggtgttc gtcgtatttt
     3541 agagttagag caaacatttc tttatctatg catgcatgat ggtggattgc tagatatgac
     3601 tgatctttgc agtaatcttg aagtaaagag acccacaata caaaattata tttttttgtt
     3661 ggaggctaca aatctcattt atcgcttacc tccctttggt tacggtaaag atattttgag
     3721 agcaaaatat aaaatttatt tagcagacgc agctatagct ccagctgtat tactgaaaga
     3781 taaaagtatt ctggatgatc ctgaatcctt aggtattgcc acagaaacag cggtgtttaa
     3841 acatctattt gctcgatatt actcgcaaaa tgtacggttt agttattggc gcggtaccaa
     3901 agaccatgaa gtggatgttg tagcacagat tggtgatcaa ataattcctt ttgaagtcaa
     3961 ataccgttca cagcatacga atgccaaagc attgaaagga ttgttagaac tatgtaagaa
     4021 aaaaagtatt gctcatggtt atgtagtaac taaatcgtta gatgacttcg gtattatgaa
     4081 agccctacca gaaattaaga caaatgttat gcaggtaccc gcaccattac tttgctactg
     4141 gatgggagaa tcagaattaa ggcagacatt tggggtttaa ttagttgata acctaatgcc
     4201 gtcaaataat ttattgaaca ctgacgcaaa atagcttatg gcaaatattt atccctcaaa
     4261 gcgtttctca ataagtcatt gttaccttga accagggtaa gttttaattt tcttaaacaa
     4321 aatggagtat ttagtcctcc cttagatgga agctctttca ctgctttgct gagcagatat
     4381 ttgacgctcc tcactgatta attcaatcca ttttttaggt gtaatgtcgt tttgtatcaa
     4441 taagttgatc gctgcactag gatattgact ttgtgtaatg gcaaaataaa agtaagcggt
     4501 caatttgctt ctgcataaag cccaaccact ttttgcgaca cattgttgca aactagcaaa
     4561 ttcaatttga tcttctttga ctaattgttg cagcttttga atcgtgtcag ggatacatgc
     4621 atcaaaaaac aatgtggtcc cttgctgtcc ccatacgtca cttaatataa ttctttttaa
     4681 aatgttcatg tcacaaattt gttgctcatg gggaataggt ttctcaattc tatgtgttac
     4741 cggttgcccc aacatggaat gtgtccattg ctgataataa gcccgaccgt aaattaatat
     4801 accacccaaa ataaaatcac aggcagttgg ggctgcatac caaagagcgg tgaagaattt
     4861 ggatttgatg acatccattt tggggtaggg atactgatgg ttattggttt taatgatatg
     4921 tttgcgatcg tattgcattt ttaaaggttc atatttaacc aaggatttag cctggtattg
     4981 attctggcca atacttatcg tttcttcctt ctttggtatt cctccatcga ctattgctaa
     5041 atgaaaatta tgatttaaaa catgttcata acccagaaaa tgacaatcgg gatttttaga
     5101 taaggtattt gctaaatgtt gtcgcaatgc ctccccgact ttaatattag gatcataaaa
     5161 atctggatgt ttggctaaat tcattaccca aaatacattg actttattct tttcaccagc
     5221 aggaatatct cgtaacatca ttccccaaca atacccaata acttcgtcgt ttttatcctt
     5281 ggcaataaaa caaatagtct ctttataatg taacattgaa gataggacga tatcccctat
     5341 aaaaggcaaa tcgccaaaat ttgctccatg ggctaaagaa tcgatacgtt taatttcccc
     5401 cattgcttta ttaagttcag cagtattatt gggatctaaa atctgaactt ctttgaagcc
     5461 atagggctta ccttccatca tttttaacga aaaattaaag gtacattctc catctaactt
     5521 tttgtggtgt ttaattttaa gaggattttg atttctgcaa tttaacagat ctgtccataa
     5581 ttgatgaatg caaactaatc tttcatcatt tggtgaggca tttcgcaaat tgattaattg
     5641 ctgggtgaaa cgatgcacat attctgtatg gagataaata gcatggctac tgaaatcaac
     5701 aacaaactct gccgtcatta attttttgat ttcgggtaag tgatattcaa caggatctgt
     5761 atatgatttt actattgcct ctaatgaact ccatttgcgt tcaagtacat cgatttttga
     5821 atacgacatc tatatttcct tgtaataaat tttcggacaa atcttaacat ggcaatttac
     5881 ttggccaaat cctatttggt cgataaaaat taggcccttt ttaaatttaa aaaggaacta
     5941 tcttcatatg gaaaactata actctcgaga ctggagtttt cattgtgctg tgttgacatt
     6001 agatagagat catcagcgat ggggtgaggg ccttggatcg gattcctatt gacacaagct
     6061 ttctaaagga atacttgaga attcagagta cagatatcct tcattgtcag tttgggcgtg
     6121 gctatgggaa gatcaactta actcgaatgt tgaaattatc acggattttc ccaaatcgcg
     6181 aaattgtttc aacactgtcg aaacaattgt cttggtcgca cttcgtcatg gtttgggttc
     6241 tactgtaatg aaccatctca cccacaatcc tttgatgatg gatggtttgt ttcagtgcgt
     6301 gtaaaatatt caattttctc aataatggat cctcatgagt atttttcgta gtgtattaat
     6361 aacgttactt tttatttcat ccgcaatggc tgcgacccct gatcaggtaa cctatttgcc
     6421 aggttggggt cccattaaga acaatcaata tgctggatat ttccctgtta atccaaaagc
     6481 gggattattt tattggtttg tagaatcaag taatccatct atggatgctc ccattgtttt
     6541 atggctgaac ggtggcccag gggcggcaag tttatatggg ttttttatgg aaaacggtcc
     6601 ttatcaggtg gataaaaatg ggaagttaac tgcccgcaaa gattcatgga caaaagcagc
     6661 taactatttg gtaattgacc aaccggtagg agtcggttac tcctatggtt cctcaaaatc
     6721 ttatggcagt gaaggggaag ctattgatca gttacaaggt gcattacagc tcatttttaa
     6781 aaaacaccca gagctctatg gaaaaccttt attcttagct ggtgaatctt acgcaggaaa
     6841 atacttaccg cagttagcta ttcgtcttct caaagataaa aatatgaatc taaaagggct
     6901 attattaggc gatccctgga ttaacccccg gctacagcaa aaagcaaata ttgattatgc
     6961 ctattatcac gggttgattg acagcaaagc cagaaaaaga gtgcaaatct tatacgaaaa
     7021 ctgtgttaaa gaaattgata aacaatcacc ctccacatca aaggcgaaca agacttgcga
     7081 acaaatacaa gaatttataa aacacgagag cgggggactt aatttagcta atattgcaac
     7141 gggggttgag ccagaagata ctaacatggt caactattta aatcaaaaag tagttagaga
     7201 agcgttgcat attcctgtga ctgtatctga attcaaaacg tttagtacgt cggcggccaa
     7261 aaaactagaa ataggtgaac aagattctgt ggctgacttg tatccacaat tgttagcggc
     7321 tggcattcgt atattaatat ataatgggct agaagatgga aaagattcaa attttctaag
     7381 tactgaactg ctgctcgctt cgctcgattg gcatggcaaa aatgcatttg caaaagcacc
     7441 aacttgcata tggcgcactg acaatgaagt gagtgggtat gcgaaaggcg cggttggatt
     7501 aacgcaggtt aaaattcgtg gtgcaggcca tctagcgcca attgaccagc cagcaagagt
     7561 tttcgattta ttcaaacatt ttataaatga taaaccctta tgctagattt ggagaaggaa
     7621 tgtatgacta ggcataggcg cgtgcagcac tttttaaacg cgctcaatat tacgtttcct
     7681 atcattcaag cgcgataacg actcccgagt tagtag
//
LOCUS       DASLOE010000146         7678 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.147, whole genome shotgun sequence.
ACCESSION   DASLOE010000146 DASLOE010000000
VERSION     DASLOE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7678
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.147"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(228..995)
                     /locus_tag="U6309_002359"
     CDS             complement(228..995)
                     /locus_tag="U6309_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HEN5505791.1"
                     /translation="MWYQNIMRNLLEKLPEASRKDGGFFLDKLFLAVKKVDNKPEMEF
                     FLGLIEKSFLKFENEKSGSVTLAKFSRYILGLTLLYLKSSNDLAIWNSDFIPSLTEIK
                     KCLEAEKAGKPFYKFINQLEVETFASLNHQLAIDFNHFSAILKRNRDLKAIQSFTEAC
                     KGLDNDVSQGFSQVMNDAEAHMINLQFAVDFDSGFGHESTDENQGNTKLQKPSHANSM
                     SMSANNLHRLGIFKLLATPDGLSDQSNLFINQNNELR"
     gene            complement(1085..2407)
                     /gene="gabT"
                     /locus_tag="U6309_002360"
     CDS             complement(1085..2407)
                     /gene="gabT"
                     /locus_tag="U6309_002360"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEN5505792.1"
                     /translation="MKKIHIKTSIPGPKSQQLMELRRQHVARGPFHATPIFVKQAKGS
                     FVEDVDGNVFLDFSSGFGVVNTGHCPDSVVNAIKLQAEKFIHTGFNIIPYEAYIKVCE
                     KLNYHTPGHFEKKSLLLNSGAEAVENAIKIARAYTGKQAVICFDHAFHGRTYMAMTLT
                     SKNKPYKHGFGPFPSEIHRAPFPYEYRWKGANCVEECFNEFTDLANFRVGVENIAAVI
                     IEPVLGEGGFIQSPALFLQKLREFCTINDIVFIADEIQSGFGRTGNLFAMNTLGVTPD
                     LTISAKGLGGGVVLAGVTGKAEIMDAAMEGGLGGTFGGNPLSCAAALEVFQIFEEGSL
                     LQNVTHLAKALQSRLSGFKEKYKVVGDVRGLGVMQAIELVKDKNTKEPNKEATVQLTQ
                     FCLEHGLVILTCGTYGNVIRLHMPLSTCVKDLEIGLSIIEEGLKRLSI"
     gene            complement(2412..3878)
                     /locus_tag="U6309_002361"
     CDS             complement(2412..3878)
                     /locus_tag="U6309_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEN5505793.1"
                     /translation="MEIYEMYIDGKFTLAKSGTTRDIFDPANGELIARVPESAKEDAI
                     LAIKAARKAFDEGEWRKSLALDRGKLLFKLADLIRANAKMLAEMETRNCGKPFPEAEF
                     DVTDAANCFEFYGGLATKIHGETMSVPANSFSYVVREPIGVCGQIIPWNFPLLMAAWK
                     LAPALAAGNTVVLKPSELTPITALELFKLIDQCGFPAGVVNLITGPGIGVGEELASNA
                     MVDKVAFTGGSVTGRKIMQAATGNLKKISLELGGKNPNIVFADCDLEMAIDGALFGAF
                     ANQGEVCSAGSRLLVERSIHKKIVEGMLKKIPNIKLGHGLDAGVKMGPLVSSAHREKV
                     ESYVKLGMEEGAKLLCGGKRPTGSEFAKGNFFEPTIFDEVKSTMRIAREEIFGPVLVV
                     IPFDNEEEAIRIANDTDYGLSGAVWTKSVTRAHRVTSQIRAGILWVNHYHPTYNEMPW
                     GGYKQSGAGRELGLYGIESYLEVKQVNINLDETPIGWY"
     gene            complement(3895..4689)
                     /locus_tag="U6309_002362"
     CDS             complement(3895..4689)
                     /locus_tag="U6309_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN5505794.1"
                     /translation="MATLKIHGVDIYYELYGQGKPLVLIAGYCCDHTFWNAMLHELAE
                     EFQVLIFDNRGIGQTRDNGDSFTLEAQADDIMAFLEQLGFRNPSILGQSMGGAIAQLL
                     ARKHGKKISKLVILNSVAKFNTRANQAMESLLNLRKENISFDLLIEAGIPWFFSSEYL
                     AEPKNIATFKENLKNNPYPQSLQNQARQFRTIPPFDSRGWLHEIKVPTLVIAAEDDIL
                     TLPAESQQLAQGIPNAQFITIPGGHSSPLEHPAIVNGAILKFLTSS"
     gene            4970..6949
                     /locus_tag="U6309_002363"
     CDS             4970..6949
                     /locus_tag="U6309_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505795.1"
                     /translation="MRYVNIELLKRIPQHLKGVMEYYPDVLLFQLNQIQYAHLWHELS
                     SAKYLYTNGFVIKPTHWLMYAFQTVKGWFGFDNHCQPEKISYVLDKLSYYGYTKQFLQ
                     PDFSLITNYSISTEISALVVKSRDDLTTAQLQNKLVNNYFKVEPYLGIDYSYQKLNPN
                     HRFGESWMHAHEWGLIPQLDPQDDNLIAEVISTLDKKGISAKDVFFLQHSKYAKAAAQ
                     YYCDKAKNTIVPSFFFRLLWTDPRPRYLALALAYDPEIAKRDAQKFIEYHLAQKEYDN
                     AFNLLGILNDSNLVLKFLLAIPETERHALIQKDTAIAAIMAKYYTEKKQYLLAIQFYS
                     NIEEISPNAAFAIEIQEQNYEKAYDIFKKYNSPDLFSMPERKSLAKIFYSEAETAYVA
                     GKTYRGNKSWEKAKQFYLQSLEQKKAAHHLDPSDEYLEDLYAHKRLYALLLIDADIDL
                     NKAEDSDIASIQKAITLLRECQSNNKEEQEHHKRALAAGLMRRVDTLREKIAFNYYSS
                     DFESIRKHKIEYQQDIAILIKTLGELIALLEGTQDKALRLQLGKAHYLLADVQCFFDI
                     NAPDINQHYKMAMKAVPGNPFYVLRVAELFEEEKSKLQKIGITQLKNMGYQVFDFLHW
                     SEERWCKRDDIIHNIKDIHMPPSEPINSSSWNFTF"
     gene            7044..7526
                     /locus_tag="U6309_002364"
     CDS             7044..7526
                     /locus_tag="U6309_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEN5505796.1"
                     /translation="MANYYGQCACGKIQFMCTGEPVFTQYCHCNKCREIASLSQKKQD
                     KSGYSLTAAYLARDFRIVSEENDFEEIIKEKAKLFLCSYCHSLVYGIALDPAQQDSIG
                     INVNNFSFDTNMPDSFKPVRHIWYASRIMDCDDQLPKFKDAPKEQFGSGELFELPNTN
                     "
BASE COUNT         2326 a         1671 c         1407 g         2274 t
ORIGIN      
        1 aagaaatccc tatttatcaa ataattggag catgtaatcc tcaattagcc cataggggat
       61 ccaagctctc catccgcaat cgtgatgctt gggaaaaata tttgtaacct tggattaatg
      121 catcaaccga atatgtcttt tcctgctcaa gtagaaaccc ctttctaccc tagctgccaa
      181 gaccgatttg ttcctgaaaa aactcaggaa caaattgatg agaaccacta tcttagctca
      241 ttattttgat ttataaacaa attgctttgg tcacttaatc catcaggcgt tgcaagtaat
      301 ttaaatattc ccagtcggtg taagttgttt gcactcatag acatggaatt ggcatgagaa
      361 ggcttttgta acttagtatt accctgattt tcatcagtgc tctcgtggcc aaaaccagaa
      421 tcgaaatcaa cagcaaactg caagtttatc atgtgcgcct ctgcgtcatt catcacttgg
      481 ctaaaccctt gcgaaacatc attatctaat cctttacaag cttcggtaaa agattgaatg
      541 gctttcaaat cacggtttct ttttagaatg gcgctgaaat gattgaagtc aatagcgagt
      601 tgatgattta aactggcaaa agtttccact tcaagttgat tgataaactt ataaaaaggt
      661 ttacccgctt tttccgcctc aagacatttc ttaatttctg ttaggcttgg aataaaatct
      721 gaattccata tggctaagtc attacttgat ttaagataga gcagggtgag ccccaaaata
      781 tacctggaga atttagctaa agtgactgag ccggattttt cattctcaaa ctttaaaaaa
      841 gatttttcaa taaggcctaa aaagaattcc atttcaggct tattgtctac ttttttaaca
      901 gcaagaaaca gtttatctaa aaaaaaaccg ccatcttttc tacttgcttc cggaagtttt
      961 tcaagcaagt tgcgcattat attttgatac cacatgctat tcacctgtaa ttaattggaa
     1021 caaatgatat caaaaactaa ttaagtgaac attaagagag ctatctcatt tcagactgtt
     1081 ttatctatat tgatagtcgc ttaagccctt cctcaataat cgaaagacct atttccaaat
     1141 cttttacgca agtagacaat ggcatgtgga gacgaatgac gttcccatat gttccacagg
     1201 tcaaaatgac caatccgtgt tctaaacaaa attgagtcaa ttgtactgtg gcttctttat
     1261 taggctcctt cgtgttttta tcttttacca attcgatcgc ctgcatgaca cccaaaccac
     1321 gaacatcacc gactaccttg tatttttcct taaatccaga aagtctggac tgcagtgcct
     1381 ttgcaagatg ggtaacattt tgcagcaggc ttccctcttc aaaaatctgg aacacttcaa
     1441 gagcagcagc acaactaagg ggatttccac caaaagttcc acccaaccca ccttccatag
     1501 ccgcatccat gatttcagct ttacctgtta caccagcaag cactacccca ccgccaagac
     1561 cttttgctga tatggtgaga tcgggggtta ctcccaaagt attcatcgca aacagatttc
     1621 ctgtgcggcc aaaaccggat tggatttcat cggcaataaa aacgatatcg tttatcgtac
     1681 aaaactcacg aagtttttgc aaaaataagg caggggattg aataaatcca ccttcaccca
     1741 ataccggctc tataataact gcagcaatat tttcaacacc gacacggaaa ttggcaagat
     1801 cggtaaactc attgaaacat tcttctacac aattggctcc cttccaacga tactcataag
     1861 gaaaaggagc acgatggatt tcggaaggaa acgggccgaa tccatgctta tagggcttgt
     1921 ttttcgaagt gagtgtcatt gccatgtacg tgcgtccatg aaaagcatga tcaaaacaaa
     1981 tcactgcttg tttgcccgta taggcacgag caattttaat ggcattttca actgcttccg
     2041 caccggaatt caaaaggagc gatttttttt cgaaatgccc cggggtgtga taatttaatt
     2101 tttcacacac cttgatatag gcttcataag gaatgatgtt aaacccggtg tgtatgaatt
     2161 tttcagcctg gagcttgatg gcattgacca ccgaatcagg acagtgcccc gtattgacca
     2221 ccccaaaccc ggaagaaaag tccaaaaaca cgtttccatc tacatcctcc acaaaagagc
     2281 ctttggcttg cttcacaaag ataggagtag catgaaatgg ccctcttgca acatgctgac
     2341 gtcgcaattc cattaactgt tgcgatttgg gtccgggtat tgacgttttg atatgaattt
     2401 ttttcattag tttagtacca accaattggt gtttcatcca ggttaatatt cacttgcttg
     2461 acctcaaggt aactctcaat gccatacaaa cctaactcac gacctgctcc gctttgtttg
     2521 tatccgcccc aaggcatttc attataagtt ggatggtaat gattcaccca aagaatcccg
     2581 gcacggattt gagatgtgac tcgatgggcc cgagtaacgc tcttcgtcca tacagccccg
     2641 gataagccat aatcagtatc gtttgcaatg cgaatggctt cttcttcgtt gtcaaagggg
     2701 atgaccacaa gtactggacc aaaaatttct tcacgagcaa tgcgcatcgt ggatttcact
     2761 tcatcaaaaa tggttggctc gaaaaaattt ccctttgcaa attcactgcc tgtcgggcgt
     2821 ttaccgccgc aaagtaattt ggctccttct tccattccaa gcttgacata actttccact
     2881 ttttcacgat gtgcacttga aaccaaaggc cccattttta ccccggcgtc caaaccatgg
     2941 ccaagcttga tatttggaat ttttttcaac attccctcta ctattttttt gtgaatagag
     3001 cgctccacta gcaagcggga acccgcagaa cagacttccc cttgatttgc aaaagcacca
     3061 aaaagggcgc cgtcgattgc catttcaagg tcacagtctg caaagacaat atttggattt
     3121 tttccgccaa gctctaacga gatttttttc aaattgccag tagccgcctg catgattttt
     3181 ctgcctgtta ccgatcctcc ggtgaaagca accttatcca ccatagcatt acttgccaac
     3241 tcctctccaa ctcctatacc cggcccagta atgaggttaa ccacgcccgc tggaaatccg
     3301 cattgatcaa tcaacttaaa taactcaagt gccgttatcg gggtcaattc tgatggtttt
     3361 aataccacgg tattgcctgc ggcaagtgcg ggagcaagtt tccaggccgc cataagcagt
     3421 ggaaaattcc atggaatgat ttgcccacac acgcctatgg gttcacgtac tacgtaacta
     3481 aatgaattcg ctggaaccga catcgtttca ccatgaatct tagtcgcaag cccaccgtaa
     3541 aattcaaagc aattggcagc atcagtgaca tcaaactccg cttcggggaa cggctttccg
     3601 caatttcgag tttccatctc tgccaacatt ttagcattgg ctcgaatcaa atcagcaagc
     3661 ttgaatagca atttgcctcg atcaagcgcc agtgattttc tccactctcc ttcatcaaag
     3721 gcttttctcg ctgctttgat agcaagtatg gcatcttctt tggcactttc tggcactctt
     3781 gcaatcaatt ccccgttagc cggatcaaaa atgtcgcgag tagttccgct tttggctaaa
     3841 gtaaattttc catcaatgta catttcgtaa atttccattt attcagcacc ttttttagga
     3901 agaggttaaa aattttagta tggctccatt gactatcgcg gggtgttcca agggactgga
     3961 atgtccacca ggaattgtaa tgaattgagc gttagggatc ccttgtgcca gttgttgact
     4021 ttctgcaggc aaggtcagta tatcgtcctc tgctgctata actagtgtgg gcactttaat
     4081 ttcatgtagc cacccgcggg aatcaaatgg tggtatggtt ctaaattgtc gcgcttgatt
     4141 ttgtaaggat tgcggataag gattattttt caaattctct ttaaaagtag cgatattttt
     4201 aggctcagcc aaatattcgc ttgaaaaaaa ccatggtatt ccagcttcta ttaataaatc
     4261 gaatgaaata ttctcctttc gcaaattcaa aaggctttcc atagcctggt ttgctctcgt
     4321 attaaatttt gcaaccgaat ttaaaataac caatttacta atttttttac catgctttct
     4381 ggcaagcagt tgagcaatag ccccacccat tgattgtccc agaatagacg ggtttctaaa
     4441 acctaattgc tccaagaaag ccatgatgtc atccgcctga gcttctaatg taaaagaatc
     4501 tccattgtct ctcgtttgcc ctatcccgcg attgtcaaat atcaatacct ggaattcttc
     4561 agccaactca tggagcatgg cgttccaaaa ggtgtgatca cagcaatagc ctgcaattaa
     4621 aaccagtggt ttaccttgcc catagagctc ataataaatg tcaacaccat gaatcttgag
     4681 tgtggccatg tcgcaaaaac tccttaattg atttttttga cgaacaaacg caacgcttca
     4741 ttcaatgact ttcgcctcaa ttatctattg tttcgatgaa ttaagtcatt tgtcaacaaa
     4801 cgtgtttgat aagctcctgc tgtcataatt gttagttttt ataaaatatg actgctcaat
     4861 taccatcaag ataattgatg cagaaattaa ttcctcttaa taatttctta attaaggttt
     4921 aatatactca tcgcttcatc gccttcctct ctattttaga tgaaaataaa tgcgttatgt
     4981 taatattgaa ttattaaaga gaatccctca gcatcttaaa ggggttatgg aatactaccc
     5041 ggatgtgctt ctgtttcaat tgaatcaaat acagtatgcc catttatggc atgagttatc
     5101 ctctgcaaaa tacctgtata ctaacggatt tgtgataaaa cctacccatt ggttgatgta
     5161 tgcctttcaa acggttaaag gctggtttgg ctttgataat cattgccagc cagagaaaat
     5221 ttcatacgtt ttagacaaac tctcgtatta tggatacaca aaacaatttc ttcagcctga
     5281 tttttcatta ataacaaatt actcaatctc tactgaaatt tctgccctgg ttgtcaaaag
     5341 cagagatgat ttaacgacag cccagctaca aaataaattg gtgaataatt attttaaagt
     5401 ggagccctat cttgggattg attattccta ccagaaacta aaccccaatc atcgctttgg
     5461 tgaaagctgg atgcacgcac acgagtgggg actgattccg caattagacc ctcaagatga
     5521 caacttgatc gcggaggtaa tcagcaccct ggataagaag gggatttcag caaaagatgt
     5581 gttctttttg caacatagca aatacgccaa agctgctgct cagtactatt gtgataaggc
     5641 taaaaataca attgtccctt catttttttt ccgcttgctt tggaccgacc cgcgccctcg
     5701 ctatctcgct ttggcccttg cttacgatcc tgaaatagcc aagcgggatg cccagaaatt
     5761 cattgaatat catcttgcgc aaaaggaata tgacaatgcg tttaatctat tgggcatatt
     5821 gaacgactcc aatctggttt taaaattttt gttagcgatt ccagaaacag aacgtcatgc
     5881 tttaattcaa aaagacacag caatcgccgc catcatggcg aaatactata ctgagaaaaa
     5941 gcaatatctg ctggcaatac aattttactc caatattgaa gaaattagcc ctaatgcagc
     6001 atttgctatt gaaattcaag agcaaaacta tgaaaaggct tatgatatct ttaagaaata
     6061 caattcccct gatctatttt caatgcctga gcgtaaatcg cttgccaaga tattttatag
     6121 tgaagctgaa acagcctatg tagccgggaa aacatataga ggaaataaaa gttgggaaaa
     6181 agcaaaacaa ttttatctgc aaagcctgga gcaaaaaaag gcagcgcacc acctggaccc
     6241 ctctgacgaa tacctggaag atttatacgc tcacaaacgc ttatatgcct tgttattaat
     6301 cgatgcggat atcgacctga acaaagcaga ggacagtgat attgccagta tccaaaaagc
     6361 aatcactctg ttgagagaat gccaatcaaa caataaagaa gaacaagaac atcataagcg
     6421 cgccctggca gccggactta tgcgccgagt agatactttg cgtgaaaaaa ttgcttttaa
     6481 ttattactct tctgattttg agtctatcag aaaacataaa atcgaatacc aacaagatat
     6541 tgcgatttta attaaaacat tgggggagtt aattgctcta ttggagggaa ctcaagacaa
     6601 agccttgcgc ttgcaactgg gtaaagcaca ttatctcctg gccgatgttc aatgcttttt
     6661 tgatatcaat gccccagaca ttaaccagca ttataaaatg gcgatgaaag cagtaccagg
     6721 aaaccccttt tatgtgctac gcgtagcaga gctatttgaa gaggaaaaaa gcaaactgca
     6781 aaaaatcggc ataactcagc tgaaaaatat gggataccag gtcttcgatt ttcttcattg
     6841 gtctgaagag cgctggtgta aacgagacga tattattcat aatatcaaag atatccacat
     6901 gccgccctca gaaccaatta atagctcatc atggaatttt acgttttaat taatagctac
     6961 aaagtataaa ataacctcgg tttgtgggct ttctttaaaa tatacttttc cattactgcc
     7021 aattgaagga gacatttaga ataatggcaa attattatgg tcaatgcgct tgtggaaaaa
     7081 ttcaatttat gtgcacaggt gagcctgtct ttactcaata ctgtcattgc aataaatgtc
     7141 gggaaattgc ttctttatct caaaagaaac aggataaatc aggttactcg ctcactgcag
     7201 cttatttggc cagagacttc aggatcgtgt ctgaggagaa tgattttgaa gaaatcatta
     7261 aagaaaaggc aaagttattt ctttgctcat attgtcacag ccttgtttat ggtattgcac
     7321 ttgatcccgc gcaacaggat agtattggga ttaatgtaaa taactttagc tttgatacga
     7381 acatgccaga ttcatttaaa ccagtaaggc acatatggta tgccagtcgt attatggatt
     7441 gcgatgatca attgcctaaa tttaaagacg ctcccaaaga gcagtttggt tcaggggaat
     7501 tatttgaatt gcctaatacc aactgataaa ccgtatccta tgcctttatc tttccaaaaa
     7561 tattcagcac acttcattga tctgactctc atattttttc aatccgacaa aacaccgttg
     7621 tgccaaaact aattcaaaca ggacttacga aaaaccccaa ggttaaggca aaaaatgt
//
LOCUS       DASLOE010000147         7449 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.148, whole genome shotgun sequence.
ACCESSION   DASLOE010000147 DASLOE010000000
VERSION     DASLOE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.148"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            422..1903
                     /locus_tag="U6309_002365"
     CDS             422..1903
                     /locus_tag="U6309_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEN5505797.1"
                     /translation="MSFFDLLINKANHLVNQIYKKNYNSITSDQLIWQHIQDCEKQFQ
                     KNQEAIYYSKDILQDLINKRLQTILLHAKTKSPWYKKTLAKINIENFTRERLEELPTI
                     NKTILMENWDAIVTDPKLSLNLVEKHLSKKNDSIDTLYLFSRYQVVSTGGSSGKRGVF
                     IYDWDEWITFHTAFVRYPLYNYERTQIVTTKLNTNPRIVSLFVTNTAIAAYSLAKTFG
                     RGNQNMYFLPMAVTPLNIVITRLNQIQPDILSAGPSYIHKVCQLVQKGQIKIEPKILF
                     VGGEPLFEQTLALIKETWPQVDIFNVFGCTEGMVGLNCRANVDEMHLNDDQCIIEPLD
                     EQNRPVDKGIMVSKMYITNLYNYTLPLIRYENSDEILFLNKTCDCGIHHQLIQTPKGR
                     PGCDFNYPGNIFVHHSLFLGALLPEKNIQEYQVEQTIDGVNIKVVTSGYINKSELQKN
                     IRLRLSKVGLVDAQVNITEVPEIKYLYSGKLNRFIPLNSADQS"
     gene            complement(2216..3532)
                     /locus_tag="U6309_002366"
     CDS             complement(2216..3532)
                     /locus_tag="U6309_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HEN5505798.1"
                     /translation="MSDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSE
                     EQLHRSGISEQLIANPYYKPYRGILDDLESFDTAPFENTTKNFELLGVQGKVMSHLTY
                     RALSTMRSQEHHSNNYIKNTAVYIGANNQPASHFLGAAYYQSIDTQKAIERYYSKVIA
                     PYISYQFGFQGSSIDLYTACSSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYL
                     YEENGLFSSDGHCHSFDVSACGTLYSNGAGLVVLKRLEDAIADNDLIHAVIIGSAMNN
                     DGNSSKEGFMAPGIHGQYTCLQSAWKNAGIEPGDLDYIAAHGASTKLGDATEIYALKK
                     AFKDVKKTHSCPISSVISNLGHTGIVSGMASIIKTVLMLKNMAVVPSINFETPNPDFM
                     LEDSPIYIATQFQDFPQIKKHYLAGVSSYGAGGSNTHLVLRSYDEN"
     gene            complement(3702..5225)
                     /locus_tag="U6309_002367"
     CDS             complement(3702..5225)
                     /locus_tag="U6309_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEN5505799.1"
                     /translation="MGSPDQYTTICTLFEEKVKRYPENTAVYLEDRQISYDDFNKQAN
                     QYARYLQRKGLPLNGIVGVQLARSFEMLVAIFAVLKAGGAYLPIDPMAPVLRNQAILE
                     DSQIKFLIVSDHSLLTATSGLQVLSIHANVSDEDCHNLNLPRKKQDLAYVMYTSGTTG
                     KPKGVLISHQALLNRILWMQNTFPLQSHDVVFHKTHICFDISVWETCWWSIVGAGVVL
                     LPPRKEHDIKLFIQMIEQYQINVIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGE
                     ALDAKSVNLFNQLFQDQKTILVNLYGPTEATIDVSCFICEKHKRFDTVPIGKPIQNTQ
                     FFVLDEQLNSTGFEPGELYISGVGLAQGYLNNPVLTQASFIDNPYLPGEKMYRTGDIV
                     RWNQEGELLFLGRKDDQVKLRGIRIELGEIQHHLLAHPNIHDAVVVCEKVDCLDHRMV
                     VFLIANNKQTKIGSAELKSFLKNRLPDYMIPEHYLWLSAFPIKENGKIDKEKLLSFI"
     gene            complement(5242..6369)
                     /locus_tag="U6309_002368"
     CDS             complement(5242..6369)
                     /locus_tag="U6309_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEN5505800.1"
                     /translation="MPNYQPQYVKQQRLFNQFIENCLHSHAKIFDKEQNIPRDFFYTL
                     AQNGFLGAGIPKQFGGQQWDELTIGIMHESFAKELCSLANILTVLGMVSKSLLQFGSK
                     AQKQIWLPRIASGETLAALALTESNIGSDLEHVETQLLDAGDEFILKGSKKYITLGQI
                     ADLFLVLANCQGQLTAVLVEKDTSGLIITPMSNFLGLRSNMLAEIFFDNCRIPKTNLL
                     GNIGIGFNCVIQTALDEGRYTTAYGCVGLGQACLDAALGYTQKRKQFDRRLDEHQLIQ
                     KMITEMVAQVKAARELCFYAGELRQQKNPAYIAETLIAKYAASKMAVFVSNHALQIFG
                     AAGFEETYPVERYYRDAKVMEIIEGTSQMHEILISKLCNNK"
     gene            complement(6439..7293)
                     /locus_tag="U6309_002369"
     CDS             complement(6439..7293)
                     /locus_tag="U6309_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEN5505801.1"
                     /translation="MKQTKLTLLGAGTMGSGITQLFAQYGFYVTLIDNLQSQLDKAKD
                     TIAKNLHYLALTQNLESTHSIETILASITFTTKLDELKQSEYIIENITENWERKKALY
                     QVLKKECSATCILGVNTSSIPITKIASLVDHPQRVIGVHFMNPAPMMPMVEVIKGYHT
                     DELTIEKTRTLLEQVHKKMIVVKDSVGFVSNRAMMIFINEAIFMVQENIASIEDIDVL
                     FKQCFGHKMGPLHTADLIGLDTVLYSLETIYSELNDPKYRPCWLLRNMVDAGLLGQKT
                     KKGFYQYE"
BASE COUNT         2306 a         1499 c         1340 g         2304 t
ORIGIN      
        1 ggtcaaggca aaaaatgttt ttaatgaggg agtttagata aactaaatga ccgaattaaa
       61 aacatttttt aacatagaga ttggggtttt tcgtaagtcc tgtcaaatta tctgtggata
      121 gcatcgactt taacatcatc accttctaac ctcgatgaga actctttgca attgcataaa
      181 tcattaaacc gcactgaaaa tgactgataa cggcactacc ttgtataata gaatatcctg
      241 cttggtttta agctataatc ttcttaggta ttttaatctt atggtgactg ccaatcaaaa
      301 attaatccaa gctacctatt gattaaaatc ccgtttagga atatcaccac taattatggg
      361 ttatctcaaa gaccatggct tatagtgtaa ggatagcgcc ctggggaaaa aggggagtaa
      421 tatgtctttt tttgacctct taataaacaa agccaatcac ttggttaacc aaatctataa
      481 gaagaattat aactccataa cttcagatca actgatatgg cagcatattc aagactgcga
      541 gaaacaattt caaaaaaatc aggaagctat ctattattct aaagacatac tgcaagacct
      601 aatcaataaa agactgcaaa caatattact tcacgcgaaa acaaaatctc cctggtataa
      661 aaaaacactg gcaaaaatca acatagaaaa ctttaccaga gagcgattag aggagctccc
      721 tactataaat aaaaccattt taatggaaaa ttgggatgca attgtcactg atcccaaact
      781 ttcattaaat ctggttgaaa agcatctgag taaaaaaaat gacagcattg ataccctata
      841 tttattctcc cgctatcagg tcgtgtcaac tggaggaagc agtgggaaac gtggcgtatt
      901 tatttatgat tgggatgaat ggattacatt tcacaccgca tttgtccgct atccacttta
      961 caactatgaa cgcacgcaaa ttgtaactac caagctaaac acaaacccaa gaattgtcag
     1021 cctgtttgta acaaatacag caattgcagc ctattcttta gcaaaaacct ttgggcgagg
     1081 taatcaaaat atgtattttc tgcctatggc tgtaacccca ttaaacatag ttatcactcg
     1141 tctcaatcaa atccaacccg atatcttaag tgccggccct tcatatattc acaaagtatg
     1201 tcaattggtt caaaaaggac aaataaaaat tgaacccaaa attctttttg ttggaggaga
     1261 gccgcttttt gagcaaacct tggctttaat caaagaaacc tggcctcaag tggacatttt
     1321 taatgtattt ggttgtactg aagggatggt tgggctaaat tgccgggcga atgtggatga
     1381 gatgcacttg aatgatgatc aatgcattat tgaacctctg gatgaacaaa atcgccctgt
     1441 tgacaaagga attatggtct ccaaaatgta tataactaat ttatataatt acactttacc
     1501 attaatccgc tatgaaaatt cagatgaaat tttattttta aataaaacct gtgattgtgg
     1561 catccaccat cagcttatac aaacccctaa aggtcggcct ggatgtgatt ttaattatcc
     1621 tggtaatatt tttgtacatc attcgctttt cttgggagca ctcttgccag aaaaaaatat
     1681 tcaggaatat caggtcgaac aaacaattga tggggttaat atcaaggttg tgaccagtgg
     1741 ttatataaac aagagcgaat tacaaaagaa tatacgctta cgattaagca aagtgggttt
     1801 ggtagatgcc caagtcaata tcactgaggt acctgaaatt aaatatttgt attcgggcaa
     1861 gctaaaccgc tttataccat tgaactcggc tgaccaatca tagtaacaga aattattttc
     1921 tctatggcaa accaagatga gaacgcgttt aaccgccatt tcttcattta ctctggtttg
     1981 ccgctgagga aacctcatac aatttggata ccaagatcag tttggcccaa tccccatctg
     2041 caccatctcg aagcctgtga aggatggcct tcgagacgtg gctttagcca ctcctcaggc
     2101 cgaacgtaaa caaattgaca tactccttgc tgtttaaatt gtatgtgagt ctctaacgaa
     2161 caaaccgcag tacgtcaact tttatcagga ataaaaatga aatatcaaca gaaccctagt
     2221 tttcatcata gctacgcaaa acaaggtgtg tgttacttcc ccctgcgcca taactggaaa
     2281 caccggccaa atagtgtttt ttaatttgag ggaaatcctg aaattgagtg gcaatgtata
     2341 taggagagtc ttccaacata aaatctggat taggagtttc aaaattaatt gaagggacta
     2401 cagccatatt tttgagcata agcacagttt taatgattga cgccataccg gacacaatac
     2461 cagtatgccc cagattacta attactgagc ttattggaca agaatgtgtc tttttaacgt
     2521 ctttgaacgc tttttttaag gcatatattt ctgttgcatc acccagcttg gttgaagcac
     2581 catgagctgc aatataatcc aggtcgcctg gctcaatccc cgcgtttttc caagccgact
     2641 gtaagcaagt atattggcca tggatccctg gtgccataaa tccttctttt gaagaattac
     2701 catcgttatt catcgcggat ccgataatca cagcatggat tagatcatta tccgcaatgg
     2761 catcttcaag ccgctttaga acaaccaatc cagcaccgtt tgaatataag gtgccacatg
     2821 ctgaaacatc aaaagaatgg caatgtccgt cggaggaaaa caagccattc tcttcataaa
     2881 gatatccaac ttcctggggt aaatcgatta agcacgcgcc tgcaattgcc atatcacatt
     2941 gatgactgtt taactctcta cagctttgga tcacggctgt cagagaagac gagcaagctg
     3001 tatataaatc aatagaacta ccctgaaatc caaattgata agaaatataa ggtgcaatga
     3061 cttttgaata atacctttct atcgcttttt gagtatcaat cgactggtag taggcggctc
     3121 ccaggaaatg ggaggcaggt tgattattgg cgccaatata aacagccgta ttttttatgt
     3181 agttatttga atgatgctcc tgacttctca tggtgctcaa agcccgataa gttaaatgcg
     3241 acatcacttt gccttgcaca cccaacaatt caaaattttt ggtggtattt tcaaacggag
     3301 cggtatcgaa tgactccagg tcatctaata tccctctgta tggtttgtaa tatggattag
     3361 cgattaattg ctctgaaata cctgaccgat gcaattgttc ttcggaaaat ttagtgactg
     3421 cttctttacc aaacttcaat aaatcccaat attcatcaag atcattagcc ccaggaaatt
     3481 ttacagccat accaacaata gcaattttgt ttgagtagtt attgtcactc ataatttatg
     3541 gctccatttc ttggccgcac caagaattta ataaacacta attaaacgac gtatgacggg
     3601 ttcataacat aaaacatagt ttatattatt tctgcaaccg gcttttaatt aagtataagt
     3661 cattttccct gattattttt atttctcggg cattgctcat gctaaatgaa actcaatagc
     3721 ttctctttgt ctatttttcc attttctttt attggaaatg cacttaacca tagataatgc
     3781 tctggtatca tataatccgg gagcctgttt tttaaaaacg atttcagttc tgcgctgcca
     3841 atctttgttt gtttgttatt agcaatcagg aatacaacca ttctatgatc aagacaatct
     3901 actttctcac agaccacgac ggcatcatgg atgttgggat gcgctaataa atgatgctgt
     3961 atttctccta attcaatacg tatgccacgt aatttgacct gatcgtcctt gcggcctaaa
     4021 aataataact caccctcttg attccaacgg actatatctc cagtacgata cattttttct
     4081 ccaggcaagt aaggattgtc tataaacgat gcctgagtca gcactggatt atttaaataa
     4141 ccctgagcta atccaactcc ggaaatataa agctctccag gttcgaagcc ggttgaattt
     4201 aactgctcgt ctaacacaaa aaattgagta ttttggatag gctttccaat gggtacagtg
     4261 tcaaaacgtt tgtgcttttc acaaataaaa caggaaacat ctattgtggc ctctgtgggc
     4321 ccgtataaat tgactaatat tgttttttga tcttgaaata actggttgaa tagattaaca
     4381 cttttggcat ccagcgcctc tccacttgaa aatacgtact ttaaagagct taagcgattt
     4441 atcgaaaaat cctgttcgat gtaacttaaa aatattcgta atacggaggg gacaaaatga
     4501 atgacattga tttggtattg ctcaatcatt tgaataaaca gcttgatgtc atgttctttt
     4561 cttggcggta aaagtactac tccggcgccc acaatagacc accaacaggt ctcccagaca
     4621 gagatgtcaa aacaaatgtg tgttttatga aatactacat catgactttg caagggaaat
     4681 gtgttttgca tccataaaat acgattaagc aaagcttgat gtgaaatcag gactcctttc
     4741 ggcttgccag tagtgcctga agtatacatc acatacgcta aatcctgttt tttcctgggc
     4801 aggtttaaat tatggcaatc ttcatcgctc acattggcat gaatgcttaa cacttgcaaa
     4861 cccgaggttg ccgtcaataa tgaatgatca ctgacaatta gaaatttgat ttggctgtct
     4921 tcaaggatgg cttgatttct cagaacaggg gccatgggat ctatcggcaa ataggcacca
     4981 cctgccttta atactgcaaa aattgccacc agcatttcaa aagagcgcgc gagttgaaca
     5041 ccgacaattc catttaatgg caaccctttt ctttgcagat accgcgcgta ttgattggct
     5101 tgtttattaa agtcatcgta cgaaatttgg cgatcttcca gataaactgc tgtattttcc
     5161 gggtatcgct ttaccttctc ctcaaataag gtgcaaattg tcgtgtactg atcaggggat
     5221 cccattgttt aataatatcc tctatttatt gttgcaaagc tttgaaatga gtatctcgtg
     5281 catttgtgag gtaccttcaa tgatctccat taccttggca tctctgtaat atcgctcaac
     5341 aggataagtt tcttcaaagc cagctgcgcc aaatatttgt aaagcatgat tagatacaaa
     5401 aacggccatt tttgaagccg cgtattttgc aattaatgtt tcagctatgt atgctggatt
     5461 tttttgctgt ctaagttctc cggcataaaa acacaattcc cgggcggctt taacctgcgc
     5521 caccatttca gttatcattt tttgaatgag ctgatgttcg tctaatctgc gatcgaattg
     5581 ttttcttttt tgagtatagc caagagccgc atccaggcaa gcttgcccta aaccaacaca
     5641 tccatacgct gttgtataac gtccttcatc caaagcagtt tgaataacac agttaaaacc
     5701 gataccgata ttacccaaca gatttgtttt aggaattcgg caattatcaa aaaaaatttc
     5761 tgccaacata ttagaacgta atcctaaaaa attagacatg ggtgtaatga ttaagcctga
     5821 cgtgtccttt tcaactaaaa ccgcagtcag ttggccttgg caatttgcta aaactaaaaa
     5881 caaatcagct atttgcccta aggtaatgta ttttttactt ccttttaaaa tgaattcatc
     5941 acctgcatcc agcaactgag tttcaacatg ctccaaatca ctaccgatat tagattcagt
     6001 taaagccaat gcagctaaag tttctcctga ggcaatccgt ggcagccaga tttgtttctg
     6061 agcttttgaa ccaaattgca aaagtgactt actcaccatg cccaatactg ttaggatatt
     6121 tgccaaagaa caaagttctt tagcaaagga ttcatgcatg ataccaatgg tcaattcatc
     6181 ccattgctgc ccgccaaatt gtttgggaat accagcgccc aaaaaaccat tttgggctaa
     6241 tgtataaaaa aagtcgcgcg gaatgttttg ttccttatca aaaatttttg catgggagtg
     6301 aaggcaattt tctataaatt ggttaaatag tcgctgttgc ttcacgtatt gaggctggta
     6361 atttggcaaa attgctctcc ctagtgtttg tttacaattt aaccatccgg gttgaaatag
     6421 cgaatggtaa actaccccct attcatattg ataaaatccc ttttttgttt tttgcccaag
     6481 aagccctgca tccaccatat ttcttaataa ccagcatggt cgatattttg gatcgtttaa
     6541 ttctgaatag atggtttcca aagaatagag aaccgtatcc aacccaatta aatcagccgt
     6601 atgcagcggc cccatcttat gcccaaagca ttgtttaaat agaacatcga tgtcttcgat
     6661 tgatgcaatg ttttcctgta ccataaagat ggcttcgtta ataaaaatca tcatggctcg
     6721 gttactcaca aaaccaacag agtccttaac aacgatcatt tttttatgta cttgttctag
     6781 taatgttctt gttttttcta ttgtcaattc gtcagtgtga tatcccttaa tgacctctac
     6841 cataggcatc atcggtgccg ggttcataaa gtgaacacct ataacgcgtt gagggtgatc
     6901 aacaagtgat gcgattttag taatggggat ggatgaagtg tttaccccca aaatacaggt
     6961 tgcgctacat tcttttttta atacctgata caacgctttt ttccgctccc agttctcagt
     7021 gatattttct ataatatatt ccgattgctt aagctcatct aactttgttg taaaggtaat
     7081 tgatgccagg atggtttcaa tagaatgtgt cgattctaaa ttttgagtta atgccagata
     7141 gtgtaaattt ttagcgatag tgtcttttgc tttatccaat tgagattgga ggttatcgat
     7201 taaagtgaca taaaatccgt attgtgcaaa aagctgagtg atacccgaac ccattgtccc
     7261 cgcacccaga agagtaagtt ttgtttgttt catgccgttt gtccaccaaa gcttatcaca
     7321 gctattcgta ttgaattata agtaaaagct caactctggc tgtttgccct aaatccaaca
     7381 taggggagtg acctattaca attatagttt actggaaata aagtgagcga ttgcatcaat
     7441 actagttaa
//
LOCUS       DASLOE010000148         7111 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.149, whole genome shotgun sequence.
ACCESSION   DASLOE010000148 DASLOE010000000
VERSION     DASLOE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.149"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(169..1323)
                     /gene="argE"
                     /locus_tag="U6309_002370"
     CDS             complement(169..1323)
                     /gene="argE"
                     /locus_tag="U6309_002370"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEN5505802.1"
                     /translation="MTTLDWLKQLISFDTTSRNSNLSLIEYLANGLNDFKVNPILIHD
                     SKEQKANLLATLPGKQGRLEGGIILSGHTDVVPVDGQIWDSDPFQATLKNNKVYGRGA
                     CDMKGFIAVVMALVPQLKEMNLDFPVHFAFSYDEEIGCLGVPSLIDKIVELNYQPRAC
                     IVGEPTLMKPVVGHKGKYSYRCQIHGVAAHSSLTNQGCNAIEHAASFISYLREMANEF
                     KKYGNRDFSYDVPYTTLTTNLIKGGNAYNTIPNLCEFVFEFRNLSTDSSEDLNQKIMS
                     YVKDQLVPNLHQDHPDSNVNLDTIAKAPGLDMPITDPFVRAAQTVCQSDKLMKVAYAT
                     EAGLFQQAEIPTIVCGPGSIEQAHRANEYVEVEQLRLCEEFIINMLRSHF"
     gene            complement(1335..2621)
                     /locus_tag="U6309_002371"
     CDS             complement(1335..2621)
                     /locus_tag="U6309_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5505803.1"
                     /translation="MNTSNTSFKTLGAICIGNAMEWYDFMIYNFMLLFIAQAFFPNHS
                     RMTSLLATMASSGVALVVRPLGGFILAVWADKYGHHKALMFVFYLMAVATMLITFTPS
                     YSTIGLAATCVIVFARLLQGLATGGEFGISSALLVNLSPSDKKGFYTSLQMVGQLLAI
                     LMGSSACLILTFYCSNEALYQFAWRIPFALGLLILPLGWILRRRLQREFTWKKEQQDT
                     GKLLILVKQQKRSLLIAFSLVAGCTGSIYTLFSYMPTYCKLYLNLSLTEAYLGTASGI
                     LVSILTIPFFGALSDRIGKKIVMLCALACYFGLIYPLLFLLNQYPSVTILILVENILG
                     LCIGAYFGVLTITVSSLFPPSVRSTCLAVSYNSAVLLFGGFAPFIITALIEYTKNPMV
                     FTYYQMTTVFISLLAVLSYQEERKLSENQPEHLVAI"
     gene            2792..3523
                     /locus_tag="U6309_002372"
     CDS             2792..3523
                     /locus_tag="U6309_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEN5505804.1"
                     /translation="MKKFILFPLISALSSTFVHAGGMGDSNSCCSTFASLEGGYTWSS
                     IDGYNFTIVGTNNTLTSTEDKQGYSGRLAVGVLSMIDDQYGFTGEVGWGYYGRTTINP
                     SVAGTLGQVPAALTIKHTLSGFDALLGVTYFQNYFSWSAKAGALIQNMQVDTSAFFTP
                     QIFPIVDNFDMKTNRTAVLPAAKLGVAYNFDSNWAITGSYLFALGANPGTTATFNPNT
                     LRSSLSIDDENPMMNAILFGVQYTA"
     gene            complement(3731..4381)
                     /locus_tag="U6309_002373"
     CDS             complement(3731..4381)
                     /locus_tag="U6309_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEN5505805.1"
                     /translation="MDRFQDRYQAGQLLGEKLKPYANRNDVVVLALPRGGVPVAAMIS
                     EAINAPLDLMIVRKLGVPGHEELAFGALAINKIKVYNEELLQYLQLSQKDINQVLSQE
                     ENELARRNRAYRGDKPFPDLKNKIVILVDDGIATGASMRAAVLALRKESPKKIIVAVP
                     VAPEDMNDIMKSVADEYICLISPVSFNAVGLWYEDFSQTEDEEVLYLLGKVYDRKV"
     gene            4713..4958
                     /locus_tag="U6309_002374"
     CDS             4713..4958
                     /locus_tag="U6309_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505806.1"
                     /translation="MNVHTMCFTRVLRYAVVTSLLFMAVSFTSVANAAQGCGEGYHRA
                     IHNGTCVLNYPGAFATPAPAHPGCWRNMWGQLRCYRY"
     gene            complement(5060..6094)
                     /locus_tag="U6309_002375"
     CDS             complement(5060..6094)
                     /locus_tag="U6309_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEN5505807.1"
                     /translation="MFRHRGILYLFLSSLIISAANPVIAKLITLGNEHLVNGRNPISF
                     CNVLFAGNFIAVLTLILIHFRELKKFQPEHLTAKNWCLIFLAALFAGFLTPTLFFFGL
                     MYTNVINVVLISNLQIPMTLLAGWIFLGEVPNIRVTTGAFLATLGVFAIVILQYWLGG
                     AGALTPNPGMKTGPLFIFLSSIPRSGEICIFLAVVSSTASTIIGFHALRRLPHYLFSI
                     LKMLLGLVLFFFIVISMFGWHHFADLFSPFLWEWMLFYGSVIIALRYYFSMLGMKHAK
                     VAEVAISSSIIPLMSIIFSYLILGEVPGISQIIGGSLILFGIYVALKGKLQAPEKTEI
                     LEKPSGFSGV"
     gene            complement(6469..>7111)
                     /locus_tag="U6309_002376"
     CDS             complement(6469..>7111)
                     /locus_tag="U6309_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5505808.1"
                     /translation="VIVSPLMSKDDAQELFKSLKEILPEEARLKVRESKQEPDQFRVV
                     LFNPEKVFSFYAKRVAELLNRFRPLPISSSEEKYYKWEYNPSSKLIEYRVTFSVLDLD
                     TEEKVKDTDEKGKELKVADQYNKKLQSIYPGLTILGSNSEDGGDDRFFLALNYSNYKA
                     MNSPPSLRDIGIEFFRKHPERYTAEVAQKIPGDMTDEFTDIEPSSTKPSSTGS"
BASE COUNT         2371 a         1405 c         1222 g         2113 t
ORIGIN      
        1 tattttaata accaattgat aaaaaagtat ttttctttaa aaccaatcaa atttcatctg
       61 atcatgttat ccacaaaaac tgtgtatatc tttgtgaata ccttataaaa gtgctagtaa
      121 aatcagcatc aaataccgat gattttttac ttaccaatct gataaggctc aaaaatgaga
      181 gcgcagcata ttgattataa attcctcaca taatcgtaat tgctctacct caacatattc
      241 atttgctcta tgggcttgtt cgatactacc tggaccacac acaatagtag gaatttctgc
      301 ctgttgaaat agcccagctt ctgtagcgta agctactttc atcagcttat cgctctggca
      361 aactgtttga gctgctctga caaaaggatc tgtaatcggc atatccaagc caggagcttt
      421 tgctatggta tcaagattaa cattagagtc aggatgatcc tgatgcaggt taggaacaag
      481 ctgatcctta acataagaca taattttctg atttaaatcc tcggagctat cagtagacag
      541 atttctaaat tcaaaaacaa actcacataa gtttggaata gtattataag cattacctcc
      601 tttgatcaga ttggtggtaa gtgttgtata tggaacatca taagaaaaat ctcgatttcc
      661 atatttttta aattcattag ccatctctcg aagatagctt ataaaagacg cggcatgttc
      721 gatcgcatta catccttgat tcgttaatga agagtgggcc gctacaccat gtatttggca
      781 gcgataagaa tatttgcctt tatgacccac aacaggcttc attaaggttg gctcacctac
      841 aatgcaagca cgcggttgat aattaagctc tactatttta tcgatcaggg aaggcacacc
      901 aagacaacct atttcctcat cataagaaaa cgcaaaatga actggaaaat ccaggttcat
      961 ttctttcaat tgtgggacta aagccatcac gactgcaata aatcctttca tatcacacgc
     1021 gccacgacca tacactttgt tatttttaag agtggcttga aaaggatcac tatcccaaat
     1081 ttgaccgtca acaggaacaa catcagtatg accagacaga ataatacccc cttccaaacg
     1141 cccttgtttt cctggcaaag tagctaacag attagctttc tgctctttac tatcgtgaat
     1201 taggatagga ttaaccttga aatcatttaa gccattcgcc aaatattcaa tcaatgataa
     1261 attagagttc cgcgaagtag tatcaaaaga aatgagttgt tttaaccaat ctaaagttgt
     1321 catattccta ccttttaaat tgcaacgaga tgttctggct gattctcaga tagcttcctt
     1381 tcctcctgat aagaaaggac tgctagcaaa ctgataaaga ctgtcgtcat ttgataatag
     1441 gtaaaaacca tagggttttt agtgtattca atcaacgccg ttataataaa tggagcaaaa
     1501 ccaccaaaca acaggacagc tgaattatag ctgacggcta gacaagtaga acgtactgat
     1561 ggtggaaata aggagctaac cgtaatggtc aacactccaa aataagcgcc gatacacaaa
     1621 cctaaaatgt tttcaaccaa aatgagaata gttacgctag ggtattggtt gagtaagaaa
     1681 agtaaaggat aaattaatcc aaaataacaa gccaaagcac ataacataac tatttttttg
     1741 cctattctat ccgacaatgc gccaaaaaaa ggaatagtaa gaatactgac taatattcca
     1801 gatgcagtgc ctaagtacgc ctctgtcagc gataaattca agtacaattt acaataggta
     1861 ggcatatagc tgaataaggt ataaatcgag cctgtacacc cggctacaag actaaaagct
     1921 atcaacagcg aacgtttttg ttgctttacc aagataagta gctttcctgt gtcctgttgc
     1981 tctttcttcc aagtaaattc tcgttgcaat cgacgtctta aaatccatcc taaaggtaga
     2041 atcaacagtc ctaaagcaaa aggtattctc caagcaaatt gatatagggc ttcattggag
     2101 caatagaaag ttaaaataag gcacgctgaa gaacccatca atattgccaa gagctggcct
     2161 accatttgta aggaagtgta aaaccccttt ttatcgcttg gcgataagtt aactaataga
     2221 gcgctggata tgccaaactc gccaccggta gcaagtcctt gcaacaatcg tgcaaataca
     2281 atgacacaag tagccgccag acctattgtg gagtaggatg gtgtaaaagt aataagcatg
     2341 gttgcgacag ccatcaaata aaaaacgaac attaatgctt tatgatgtcc atacttatct
     2401 gcccaaactg ctaaaataaa accaccgaga ggccggacta ccagagccac accagaactt
     2461 gccattgtag ccaagaggga ggtcatacga ctatgattgg ggaaaaaagc ttgggcaata
     2521 aaaagcagca tgaagttata aatcatgaaa tcataccatt ccatagcatt accaatacat
     2581 attgctccca aggttttaaa ggaggtattt gatgtattca tgtttcctta cgagggtaaa
     2641 tttagagcaa agcacatact agtagaatat aaaaaaaatt accatgcttt ataagaaact
     2701 aacatcaata ttaattttac tggtaattcc ctatcaactg atttaaatta ttgactttta
     2761 caataataaa catggaatga gttactaaat catgaaaaaa tttatcctgt tccctttaat
     2821 ttccgccctg tccagtactt ttgtccatgc tggtggtatg ggtgattcca actcttgttg
     2881 ctctactttt gcgtcacttg agggtggata cacctggagt tccatagatg gatacaactt
     2941 tactatagtc ggtaccaaca atactctcac ctcgacagaa gataaacaag gttatagtgg
     3001 ccgtctggct gttggtgtgc ttagtatgat agatgatcaa tatggattca caggcgaagt
     3061 aggctggggt tactatggta gaacgaccat taatccaagt gtcgcaggaa ctttaggcca
     3121 ggttccagct gccttgacga tcaagcatac cttgagcgga tttgatgccc tactcggcgt
     3181 tacctatttt caaaactatt ttagctggtc tgccaaggcg ggcgcgctca ttcaaaatat
     3241 gcaagtagac actagtgcct ttttcactcc ccaaatattt cctatagtgg ataatttcga
     3301 tatgaaaacg aacaggactg ctgttttgcc agcagcgaag ctgggagttg cctataactt
     3361 tgattctaac tgggctatta ccggctctta tttgttcgcg cttggagcaa atccaggaac
     3421 aactgcaact tttaatccta acacattaag atccagtctg agcattgacg atgaaaatcc
     3481 catgatgaat gctattttat ttggggttca atataccgct tagtttaata ttatttaaaa
     3541 acgtcacctt ttatatgggg tcaaatattt acaaacttat aaaagtcttt aatccttttg
     3601 aagtttgata ataaacacgt tggccccaag actttttaac ttattccttc aacaggtctt
     3661 cacaggactc aaaagaaagg agataaaagc ttctgccttt cattttgtca tggtttgtca
     3721 tttcttattt ttagactttt ctatcgtata ctttgccaag taaatataat acttcttcat
     3781 cctcagtctg ggagaaatct tcataccaaa gacccacagc gttaaaagac acgggggata
     3841 taagacaaat gtattcatcc gcaacacttt tcattatgtc attcatatct tcaggtgcta
     3901 caggaaccgc tactataatt tttttaggtg actccttacg taaagcaaga acagctgcac
     3961 gcatactagc acctgttgca ataccgtcat ctaccagaat tacgatttta ttttttaaat
     4021 caggaaaagg tttgtcacca cgataagctc tattccgcct tgctaattcg ttttcttctt
     4081 gagaaagaac ctgattaatg tctttttgag aaagttgcaa atattgcaac agctcttcat
     4141 tataaacttt tattttatta atggccaaag caccgaaagc taattcttca tgcccaggaa
     4201 cacccagttt gcgaacaatc attaaatcta atggagcatt tatagcctca gaaatcattg
     4261 cagctacagg cactcctcca cgaggtagtg caagcactac gacatcattt cggttcgcgt
     4321 aaggcttaag cttctctcct aataattgtc cagcttgata acgatcttga aagcgatcca
     4381 ttttcccact caattatctg tcctgctact tttattgtag ttcagatctg gaaattatcc
     4441 tgctgtcagc aagagtttat aatgattaat cttctctgaa tttaagctaa taattatcag
     4501 tacactattt catcatttga atccatttca aataaagtga atggctgtgt acatcaaaat
     4561 agtgcatcaa ggaatataaa gaatggatta atttatagag tttaaagatc accaaccaaa
     4621 aattatgatt atattttagg cataatttaa aaattgactc tatacttatg ctgtatgcta
     4681 aaaaataatt gaagcaatat aaggagagta caatgaatgt gcataccatg tgttttacga
     4741 gggtgttacg ttatgctgtt gtgacaagcc ttctgtttat ggctgtgtct tttacatcag
     4801 tggcaaatgc ggctcaggga tgcggggaag gttatcacag ggccattcat aatggtactt
     4861 gtgttctaaa ctatccaggc gcctttgcaa cccctgcacc agctcatcca ggctgctggc
     4921 gtaatatgtg gggacaattg cgttgctata gatactaatt caaacttgaa aatacaaaca
     4981 tatcaaacct tatttcttgc tgttatattt ttgatgtaac tagcaaaaat aaggttttat
     5041 tcatagaaaa atagtcattc taaacacctg agaaacccga aggtttttcc aatatttcgg
     5101 ttttctcggg agcctgcaat tttcctttta aagcaacgta tatcccgaac aaaattaaac
     5161 ttccacctat gatttgcgaa ataccgggaa cttcacctaa tatcaaatag gaaaaaataa
     5221 tagacatcag aggaattatt gatgaactga ttgcaacctc tgcaactttt gcatgtttca
     5281 tgcccagcat actgaaataa tatcgcaaag caataataac actcccataa aagagcatcc
     5341 actcccataa aaatggggaa aaaagatcag caaaatgatg ccagccaaac atagaaatca
     5401 ctataaaaaa gaataaaaca agccccaata acatttttaa aatactaaac agataatggg
     5461 gtaatcttct aagtgcatga aaaccaataa tagttgaagc agtactggaa actactgcca
     5521 aaaaaataca aatttcacct gaacgaggaa tagagctgag aaaaataaaa agaggacctg
     5581 ttttcattcc agggtttgga gttaaagcac ctgctccccc caaccaatat tgaagaataa
     5641 ctattgcaaa tactcccaag gtagctaaaa aagccccagt ggttactcga atattaggca
     5701 cctctcccaa gaaaatccag ccggccaata aagtcatagg gatctgtaga ttggaaataa
     5761 gaacaacatt aatcacattg gtgtacatta atccaaaaaa aaataaggtg ggcgttaaaa
     5821 aacctgcgaa taaagcagcc agaaaaatta agcaccaatt tttcgctgtt aaatgttctg
     5881 gttgaaattt ttttagctct ctaaaatgaa ttaaaatcaa agtgagcact gcaatgaaat
     5941 ttccggcaaa taggacatta caaaacgaaa ttggattacg accattgacc aaatgctcat
     6001 tcccaagagt aataagtttt gctatgacag ggttagcggc acttattatg agcgaactaa
     6061 ggaataaata tagtattccc ctatgtcgaa acagtctctt aattttctcc ccctatatcc
     6121 ttatcaaggc cttaatttta tatactcaat tttacctgta tcaatatctt ttttcagatc
     6181 agacgaagca aaaaatagta tctcttgatt agctcctccc aatgcacaat cgaatggcat
     6241 ggatagagta tgcacgactt taataatctt acctttcgaa tcaatacata aaacttcttt
     6301 agattcctta agtgcagcta aaaatttact ttataaatct cccttacagc aaaatatgct
     6361 gcaataagag aagagaaaag gaaatgatac aattaaatcc acgtttattt acaagataaa
     6421 aagccacagt ttctatttat ccgttactcg tctatgctca ataaaaaatt agctgccggt
     6481 tgaagatggt ttagtagaag atggttcgat gtcagtaaac tcatccgtca tatctcctgg
     6541 tattttttgg gcaacttctg ctgtataacg ctcgggatgt tttctaaaaa actcaatccc
     6601 tatatcacgt aaactgggag gactattcat ggctttataa ttcgaataat ttaaagccag
     6661 gaaaaacctg tcatcccctc catcttctga attactccca agaattgtga gtccaggata
     6721 gattgactgc agcttcttat tatattgatc tgccactttt aattctttac ctttctcatc
     6781 agtatcttta actttctctt ccgtatcaag atcgagaaca gaaaaagtaa cgcggtattc
     6841 tattagtttc gatgagggat tatattccca tttgtaatat ttctcttcag aagaagatat
     6901 cggtaatggt ctaaatcgat ttaacaattc tgcaacacgc ttggcataaa acgaaaaaac
     6961 cttttcagga ttaaagagaa cgacacgaaa ctgatctggt tcttgctttg actcacgtac
     7021 ctttaatcgt gcctcttcag ggagtatttc ttttaatgat ttaaataatt cctgagcatc
     7081 atctttactc atcaaaggag atacaataac t
//
LOCUS       DASLOE010000149         7084 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.150, whole genome shotgun sequence.
ACCESSION   DASLOE010000149 DASLOE010000000
VERSION     DASLOE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.150"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            81..845
                     /locus_tag="U6309_002377"
     CDS             81..845
                     /locus_tag="U6309_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEN5505809.1"
                     /translation="MKDIIIASFYKFIPLNDFESLREPILTKMHEIGIKGTIILAHEG
                     VNGGFAGNREQMNVFYDYLRSDSRFADLHFKETYDNKNPFDKAKVKLRKEIVTMGVQK
                     VDPSYNAGTYLSPEEWHQFIQDPNVILLDTRNDYEYELGTFKNAINPDIENFREFPDY
                     VQRNLIDKKDKKIAMFCTGGIRCEKTTAYMKELGFEHVYQLHDGILNYLESIPESESL
                     WEGKCFVFDDRVAVDQKLDRVYPQLPQDYKYEREQK"
     gene            957..1427
                     /gene="smpB"
                     /locus_tag="U6309_002378"
     CDS             957..1427
                     /gene="smpB"
                     /locus_tag="U6309_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEN5505810.1"
                     /translation="MTTKKQPDSTIALNRKAGFDYFIEDQYEAGLVLEGWEVKSLRAG
                     KINLSDSHVIIKYGEAFLLGAQIQPLPTASTHFIPDPIRTRKLLMNKKELNHLIGSVE
                     RQGYTIVPLSLYWKKNKIKIKIALAKGKKEHDKRDTIKDREWQRDRSRIMKKNT"
     gene            1439..1903
                     /locus_tag="U6309_002379"
     CDS             1439..1903
                     /locus_tag="U6309_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEN5505811.1"
                     /translation="MKIGEMVPDFAFNATNNVQGHLNDYKGQIIVLYFYPKDATPGCT
                     TEGQDFRDAYSQFKDLNTVIFGISRDSLKSHEQFKSKQNFPFELISDSNETLCQMFDV
                     IKMKSMYGKQVRGIERSTFIIDTQGVLTHEWRKVNVKGHVEEVLDTVRSLAG"
     gene            2029..3228
                     /locus_tag="U6309_002380"
     CDS             2029..3228
                     /locus_tag="U6309_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5505812.1"
                     /translation="MFIRNLQLFIVVASCILVLIGTDILLPSLPNIAHALSVSSNEAK
                     MLISIFMIGQFSTVLIWGVIADRLGRRKTLFLGMLIFFIGSIFSLKADSIQLLLACRF
                     LQGTGAVVVPVAGWALVQDLFPKDEGARIMTWIGTLTAVIPLFAPALGGQLDVLYGWR
                     SNLYCIAFYSFILCVIMLFLPRTIPVQKTTTADLKETLSIYIRIIKNKTFISYIALFG
                     LLNCGEWCFLTVAPFYYSHAHIAADTMGILLMITSMGFVCGSLLASRLFNRLGIDKII
                     ALGIQLAVMSSLMLLIGEYFHWSKYQLYNAITIGVYILSSALLWGATTSRALQCFDDC
                     RGSASAIRSLILLCFAAFGTYSGRLVNHNSLFPVGLFLLFMAFIAFIVFNNKELKTER
                     HMEGIPI"
     gene            3383..4789
                     /locus_tag="U6309_002381"
     CDS             3383..4789
                     /locus_tag="U6309_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEN5505813.1"
                     /translation="MDNTNTGLKLFVLDTNILMHDPTAIYHFDEHDIYLPMVVLEELD
                     SHKTGTSELARNVRQTNRMLVELMSNATHDQIVSGLPIPSYINSSNKKCSGRLFFQTD
                     EFDQVVPSTLPGHKVDNTILATALGLQKKYYGKRQVIIISKDINLRIKAGILGIHAED
                     YYNDQVLDDVNLLHRGLHILDNNFWDSHAKDMGSWQESGKTFYKVSGPLVSQWNPNDC
                     LSTEDDQFQALVKKIDGPNAVVQLIRDYTQPKHSVWGITARNREQNFSLNLLLDPDID
                     FVSLQGSAGTGKTLLTIAAGLTQVMDQNRYSEILMTRVTIPVGEDIGFLPGTEEEKMT
                     PWMGALMDNLEVLHSSQVGGSFGRGATQDLLQNKIKIRSLNFMRGRTFLNRYIIIDEA
                     QNLTSKQIKTLVTRAGPGSKIICLGDIKQIDTPYLTETTSGLTFAVDRFKSWEHSAHM
                     TLTRGERSRLAFYAAEHL"
     gene            4812..5825
                     /locus_tag="U6309_002382"
     CDS             4812..5825
                     /locus_tag="U6309_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine monophosphate reductase"
                     /protein_id="HEN5505814.1"
                     /translation="MTDQAITFDDVLLVPSYNHHESRRVVETTSTDRLGKLTLNLPVI
                     SANMDTITESNMANFMHSKGAMGALHRFMTIEENIQEFKKCKGPVFVSVGCTENELQR
                     AEALRDAGADFFCVDVAHAHAKYVGKTLKSLRQLLGSRCIMAGNVATYAGADYLASCG
                     ADIIKAGIGGGSVCSTRIKTGFGVPMLTCIQDCSRADRSIVADGGIKTSGDIVKALAF
                     GADFVMIGGMLAGSAPTPGEVIQKDDGSKVKRYRGMASREAQEAFLGQMHEWKTAEGV
                     ATEVPFKENPDGIIADIIGGLRSGLTYAGADSISELQRKLNYVIVTQAGRIESLPHKL
                     LEG"
     gene            complement(5856..6941)
                     /locus_tag="U6309_002383"
     CDS             complement(5856..6941)
                     /locus_tag="U6309_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2844 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505815.1"
                     /translation="MAETPKPKSQTPTPKPKPKPTDEQTVEPAPQQSTLGKVGSVIGG
                     LAKKGLGKLADNIGKQFGSNAPQPGAENKPAEGQKKDDDKKDPQMEMIERYMKLVEQW
                     QGGINKPFYWVGGKISAGASFLVDKAVEGIKNKLAESEQPKPNPNSSTQQSNSSSSSS
                     SSSTTPTPTSSSSSGSQSSSPPTTSSGSDSQSSSPPTTSSGSDSQSSSPPTDSSSSGS
                     DSQSSSPPTDSSSSGSDSQSSSPPTDSSSSGSDSQSSSPPTDSSSSGSDSQSSSPPTD
                     SSSSGSDSQSSSPPTDSSNSSSSSSSDTSSQLSPPPDSNTNTSTPDLGSQTKYDETSS
                     SSNSGSGQSSGSGSSSDVPTPSQSMPG"
BASE COUNT         2059 a         1398 c         1552 g         2075 t
ORIGIN      
        1 ttaattcatt cctattgatt aaattgtgag taaatgataa tttttgcgaa ttgatttttt
       61 gttaatcctt aggttacagc gtgaaagaca ttattattgc atcattttat aagttcattc
      121 ctctcaatga ttttgaatca ctgagagagc ccatactcac taaaatgcat gaaataggca
      181 taaaaggaac tatcatcctg gcccatgagg gcgtcaacgg cggatttgcc gggaatagag
      241 aacaaatgaa tgttttttat gattacctgc gttctgattc gcgctttgct gacctgcatt
      301 ttaaagaaac ctatgacaac aaaaaccctt ttgataaagc gaaagtgaaa ctgcgtaaag
      361 agatagtgac catgggcgtc cagaaagttg acccctctta taatgcaggg acctatctaa
      421 gccctgagga atggcatcaa tttattcaag atcctaatgt tattttatta gacacccgca
      481 atgattatga atacgaactg ggtactttca agaatgcaat taatcctgac atagaaaatt
      541 tcagggaatt tcccgactac gttcagcgca atttaattga taaaaaagac aagaaaatag
      601 ccatgttttg tactggggga attcgttgcg aaaaaacgac agcctatatg aaagagctgg
      661 gctttgagca tgtttaccaa cttcatgatg gcattctaaa ttacctggag tctattcctg
      721 aaagcgaatc actttgggaa ggcaaatgtt ttgtttttga tgaccgcgtt gcagtggatc
      781 aaaaattaga tcgtgtttat ccacagttac ctcaggatta taaatacgaa agagagcaga
      841 aataacgtag aatatacttt tttgccataa gccgttcaag gcaacaaaat taagaaaagc
      901 agttcaatgg gatagtacat cagttatact gtttttttca acaaatcaga tgaattatga
      961 ctaccaaaaa acaaccagat tccaccatag ccttgaatag aaaagctggt tttgattact
     1021 ttattgaaga tcaatacgaa gcaggcctgg ttttggaagg ctgggaagta aaaagtctgc
     1081 gtgctggaaa aatcaatttg tcggattcac acgtgataat caaatacggt gaggcattcc
     1141 tattgggcgc ccaaatacag cctcttccca ccgcatccac tcattttatt cctgatccga
     1201 tcaggacgcg caagctattg atgaataaaa aagaattaaa ccatctcata ggaagtgttg
     1261 aaaggcaagg ctataccata gtccctcttt ctttgtattg gaaaaaaaat aaaattaaaa
     1321 taaaaattgc tctggctaaa ggaaaaaaag agcatgacaa aagagacacg atcaaagaca
     1381 gagaatggca aagggatcgt tcaagaataa tgaaaaagaa cacttgagga gatggtttat
     1441 gaaaataggt gagatggtcc ccgattttgc ttttaacgcc acgaacaacg tgcaaggaca
     1501 tcttaatgat tacaaaggac agatcattgt actttatttt taccctaaag atgccactcc
     1561 gggctgtacg actgaaggtc aagacttcag agatgcctac tcacaattta aagatttaaa
     1621 tacggtgatt tttggtattt ccagagatag tttaaaatct catgaacaat tcaaaagcaa
     1681 acagaatttt ccttttgaat taatcagtga tagcaatgaa acattatgcc aaatgtttga
     1741 tgtgatcaag atgaaatcca tgtatggcaa acaagtcaga ggaatagagc ggagcacttt
     1801 cattatagat acacaaggtg tcttgacaca cgaatggcgt aaagtgaatg taaaagggca
     1861 cgttgaggaa gttctagaca cggtgcgatc tttggcagga taaataagaa taatccctga
     1921 tagggattga tgctttaaaa aaatactttt tatagagtat ttaattaatt tttgctatta
     1981 ttgttgcgga taaatacatc aaatacgccc ttttcttaag agttcaatat gtttatccgc
     2041 aacctgcaac tttttattgt tgtcgcctct tgcatactgg ttttaattgg tacagatatt
     2101 ttgttaccaa gcctgcctaa cattgctcac gcactgtctg tctcatcaaa tgaagccaaa
     2161 atgctcattt ccattttcat gatagggcaa ttttctacag ttcttatttg gggcgttatt
     2221 gccgaccggc taggcagaag aaaaacatta tttctgggga tgcttatttt ctttataggc
     2281 tcaatcttca gtttaaaagc cgattcaatt cagttgttac tggcttgtcg attcttgcaa
     2341 ggtactggcg cagtcgttgt ccctgttgca ggttgggctt tagtccagga tctcttcccc
     2401 aaagacgaag gagctcgcat catgacctgg attgggacac tgactgcagt gattccactc
     2461 tttgcccccg cattaggggg acaattggat gtgttatatg gttggcgttc aaatctttat
     2521 tgtatcgcat tttactcttt cattttatgc gtcattatgc tgtttttacc cagaactatc
     2581 cccgtgcaaa aaacgacaac tgctgacctg aaggaaacac taagcattta cattcgaatt
     2641 ataaaaaaca aaacatttat ttcctatatc gcactatttg gattattaaa ctgtggcgag
     2701 tggtgcttcc tgactgtagc tccattttat tattcgcacg cacatattgc tgctgacacc
     2761 atggggatat tattaatgat tacttccatg ggatttgtct gcggatcgtt actggcctcc
     2821 cgcttattta atcgcctggg gattgataaa atcatagcat tgggaattca gttggctgta
     2881 atgagcagcc tgatgttgtt aataggagaa tactttcact ggagcaagta ccaactctat
     2941 aatgccataa ctatcggcgt ctatattcta agctcagcct tgctatgggg cgcaaccaca
     3001 tccagagccc tgcaatgctt cgatgattgc agaggctcag cctctgcaat tcgcagttta
     3061 atattactgt gctttgctgc ttttggcact tattcaggtc gcctggttaa tcataatagc
     3121 ctgttccctg ttggattgtt tttacttttt atggccttca ttgcattcat tgttttcaat
     3181 aataaagaat tgaaaacaga acggcacatg gaaggcatac ccatttaact taaaaacaga
     3241 cacttaataa tataattctt gacggaacca ccatagctca gcacaatgaa catagaattc
     3301 agggatttca atgtactccc tgcctacgca ggagactgat tccacttgaa ttgaagaata
     3361 ctttagctga cagggtgaac ctatggataa taccaatacc ggacttaagc tgttcgtgct
     3421 cgatactaat attttgatgc atgatcctac tgctatctac cattttgatg agcatgatat
     3481 ttatttgcca atggtggtgc ttgaagaatt ggatagtcat aaaacaggga catccgaatt
     3541 ggcgagaaac gtaaggcaaa cgaaccgcat gttggtagag ctaatgagta acgccactca
     3601 cgatcaaatt gtctcaggac tgcctatccc atcttacatc aatagcagca acaagaaatg
     3661 cagtggacga ttgttttttc aaacagatga atttgatcaa gtggttcctt caactctgcc
     3721 gggccataaa gtagacaata caatactggc aaccgcttta ggtctgcaaa aaaaatatta
     3781 tggcaaaaga caagttatca ttatttcaaa agacatcaac ttgcgtatta aagcagggat
     3841 attaggtatt catgctgaag attactacaa tgaccaagtg ctggatgacg tcaatttact
     3901 tcacagagga ttacatattc tcgataacaa tttttgggat agtcatgcca aagatatggg
     3961 atcctggcaa gaaagtggta aaacctttta taaggtaagc ggtcctttgg taagccaatg
     4021 gaatccaaac gattgcctta gtacggaaga tgaccagttt caagctttgg tcaagaaaat
     4081 tgatgggcct aatgccgtag ttcaactcat tcgtgactac actcaaccca aacattcagt
     4141 ctggggaatc acggcaagaa accgtgaaca aaatttttcc ctaaacttgt tactggatcc
     4201 cgatattgat tttgtcagct tgcaaggctc agcaggtaca gggaaaacac tattaaccat
     4261 agccgcagga ttaacccagg tcatggatca aaatcgatac agcgagatat taatgacaag
     4321 ggtaacgatt cctgttggcg aggatatagg attcttgccg ggtaccgagg aggaaaaaat
     4381 gactccgtgg atgggcgctt taatggataa tcttgaagtg cttcacagct cgcaagtcgg
     4441 cggcagtttt ggacgcgggg caactcagga tttactgcaa aacaaaatta aaatacgctc
     4501 gttaaatttt atgcggggaa gaactttttt aaaccgctat attataattg atgaagcgca
     4561 aaatctgaca tcaaaacaaa ttaaaacctt ggttacaaga gcaggcccag gtagtaaaat
     4621 tatttgtctg ggggacatta agcaaattga taccccctat ttaactgaaa ccacttccgg
     4681 actgactttt gccgtagacc ggtttaaaag ttgggaacac agcgctcata tgactttgac
     4741 tcggggagag cgctcaaggc tggcttttta tgcggctgag catttatagt atttgtttat
     4801 gaggattaat aatgactgat caagcgatca cttttgatga tgttctgctc gttccatcgt
     4861 acaaccatca tgaatccagg cgagttgttg aaacaaccag taccgacagg ttaggtaaac
     4921 tgactttaaa tcttccggta atcagcgcca atatggatac aattaccgaa agtaatatgg
     4981 ctaattttat gcacagcaaa ggagccatgg gcgctttaca ccgatttatg accatagaag
     5041 aaaatattca ggaatttaag aaatgcaaag gacctgtctt tgtatcggtt ggatgtactg
     5101 aaaatgaatt acaacgagca gaggcactgc gagatgcagg agccgatttc ttttgtgttg
     5161 atgtagcgca tgcccacgcc aaatacgttg gcaaaacatt aaaaagctta cgtcagttat
     5221 tgggaagtcg ctgcattatg gctgggaatg tcgccactta tgccggggca gattatcttg
     5281 cgtcttgtgg cgctgatatt attaaagcag gtattggagg aggctcagta tgcagcaccc
     5341 gaattaaaac cggctttggc gtccccatgt tgacctgtat tcaggattgc tcacgtgccg
     5401 atcgttccat cgttgctgat ggcggcatca aaacatcggg tgatatcgtc aaagcattgg
     5461 cctttggcgc tgattttgtg atgataggcg gcatgttagc tggctctgcc cccacgccag
     5521 gagaggtcat tcaaaaagat gacggaagca aagtcaaacg ctatcgaggc atggcatccc
     5581 gagaagcgca ggaagcattc cttggacaaa tgcatgaatg gaaaacagca gaaggtgttg
     5641 cgactgaagt cccatttaaa gaaaaccctg atgggattat tgctgatatc ataggcggat
     5701 tgcgctctgg gcttacctat gccggcgccg actccatcag cgaattgcaa agaaaattga
     5761 actatgtgat tgtcacccag gctgggcgca tagaaagttt gcctcacaag ttgctggagg
     5821 gataactcac caacccggat aatctgtttt cacatctatc caggcataga ctgactaggt
     5881 gttgggacat cagaagatga gcctgagcca gaagattgac cactcccgct gttagaactg
     5941 ctggatgttt cgtcatactt tgtttggcta ccaagatctg gcgtacttgt attagtattc
     6001 gagtctggtg gcgggctcag ttgtgatgaa gtatcactac tgctgctact gctactgtta
     6061 ctactgtctg taggcggtga tgaggactga ctgtcgctgc cactgctgct actgtctgta
     6121 ggcggtgatg aggactgact gtcgctgcca ctgctgctac tgtctgtagg cggtgatgaa
     6181 gactggctgt cgctgccact gctgctactg tctgtaggcg gtgatgaaga ctggctatcg
     6241 ctgccactgc tgctactgtc tgtaggcggt gatgaagact ggctatcgct gccactgctg
     6301 ctactgtctg taggcggtga tgaagactga ctgtcgctgc cactgcttgt tgtaggcggt
     6361 gatgaagact ggctgtcgct gccactgctt gttgtaggcg gtgatgaaga ctggctgccg
     6421 ctgctgcttg acgaagtagg tgtgggggta gtactactag aactggaact cgaactggaa
     6481 ttggattgtt gcgttgagga gttgggattt ggtttgggtt gctctgattc agccagttta
     6541 tttttaatcc cttccactgc cttatcaacc agaaaactgg caccagctga tattttgcca
     6601 cccacccagt aaaaaggctt gttgataccg ccttgccatt gctcgaccag cttcatatac
     6661 ctttcaatca tttccatttg cggatctttt ttgtcatcat ccttcttttg tccttccgca
     6721 ggcttatttt cagcaccagg ctgaggagca tttgagccaa attgcttgcc gatattatcg
     6781 gctaatttgc ctaacccttt tttagcaagt cctccgatta cactacctac tttcccaagc
     6841 gtactttgtt gaggagctgg ctcaacagtc tgctcatccg ttggttttgg tttcggcttc
     6901 ggtgttggtg tctgtgattt gggttttgga gtttctgcca tgttaattta ccatatctgt
     6961 tgatatctaa attatagtac ctcatcaaga ttttgtccga ggttactttt ccaattttac
     7021 tgttcaggga tatatttttt attgttatat aatataataa aacaaatgaa tcaattaaat
     7081 acaa
//
LOCUS       DASLOE010000150         7029 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.151, whole genome shotgun sequence.
ACCESSION   DASLOE010000150 DASLOE010000000
VERSION     DASLOE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.151"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            5..1864
                     /gene="ravS"
                     /locus_tag="U6309_002384"
     CDS             5..1864
                     /gene="ravS"
                     /locus_tag="U6309_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavS"
                     /protein_id="HEN5505816.1"
                     /translation="MPGIKKFNLTYQQVPKIFSLPESVFLSKDAELIPEEVLKQTKIQ
                     KKIVAKTIEQIDKDLERTCYVDFDGLTVAQLKSDWLTVNPQGELVLSEGVYPSFSQGD
                     NTGAVIGEILQTKHFSLPLSEEDPKAFMEVTRNTTRICFSYKNSAGQPSGFSLVAAPN
                     GDSEGKHKGWIISVIQNTVGNYEDRKVSVLASPNYVRSEAQSTVDMDTPVAIERELKR
                     IINNDKVSSLIKGIFKEDGTLNADYIRQLASRIKANRNIDDRDRKSAQFNILIELAEQ
                     LDDEKLRHYINSIKADMAADIEFFKDGIYEQHLSELSNKISLSLSKMPSVNKKTTEIG
                     RILNNISERYAQIKMIEDAQRKNPELAHIFKAMAQKIKSDFHDIKFDGTRQDNRNILL
                     LSQFEVASVEQKIRNYYQTNPLVKQYFIDKDKANHHLQVELSRLSTPKNEGFFSRNKD
                     SFGFSIFMGLIVGVSAALFLTGVLAPIGVALIAVGAALITTGLVGSIDIYINEKEYAK
                     ETSHYSNEVKCLRSAHREHLVRLEKNLHEQINLSDFLTPDKPEFLSGIRVNKKKPLTP
                     ERVAHALWLQDKSARDTSVAKNRFSIWAAKQENLDFGIEEVESVIKQRYVMKS"
     gene            1956..4343
                     /locus_tag="U6309_002385"
     CDS             1956..4343
                     /locus_tag="U6309_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN5505817.1"
                     /translation="MLTDTQIQQAINNLNILIAHHNLPLSCFSFKNEQVTRNWLFKSE
                     SIHATALSNYLKKIINEVSSGEVDKTHSLYASLFITLQNFLHLQPNRVIEGLNFNLEN
                     SQYLKRKNLIIKRLEHFHIENQFRDDLKRHLLPEIQYDTYTGHYTSPDDDKAHQHGSE
                     AQRIFFSTQWERLKALCNWLLAKMGIHIFETARYKEHDYLDSEIYADDPAIEPGVSPQ
                     QSATHYWIGHASNLMTIPAHDSPLHVVTDPVEGDLAPFLYPRMTKEGSLIDGTGSKKL
                     PKIDIVVISHNHHDHVSQSTLKRLLKQQPKMVVPEGDESLFLGMGFTDVVGLKWWEQA
                     KITDFQGNELLRITAVPARHWSGRSLTDAHCSAFNGYVLHANQLDGDIYFAGDTALMD
                     DMLSQPIFEKFNIVTSIQPGGPDERREDMESTHQSSADAILMHFKILAAQYQKMKEKN
                     ESLSLDEFLMENQHIKTLYNHTATFKLGNLRLKDTYYSYQRMVAAFREDAQWRADHLP
                     KHEQKVYDHIQLLISNMVFKNNQRLSNRQIVDLILGKVVIPKIGQRQPLYFKEKSIKT
                     EAFQYRNLITNRRALLEFDMLLQESIAESNEFFNIKRLILNLLRAYQNPWHAFFSRTH
                     LGVGNYEQDIRNCQSNSDLLAVLNQMERNLGKRNPHGHMQSLIYYAKWVIDFSNKYQH
                     ETLMKFKEYFACQQIRKLADQEIHHAGLIVGLGDRQAKQQRFKVLSNELADLPVDVTE
                     YRKAIDNWLATRVNDGQSTQQLLSENRSSVFAKKVTRGESTVLHIKTTLDLAVSS"
     gene            complement(4474..4561)
                     /locus_tag="U6309_002386"
     tRNA            complement(4474..4561)
                     /locus_tag="U6309_002386"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4525..4527),aa:Ser,seq:tga)
     gene            4727..5500
                     /locus_tag="U6309_002387"
     CDS             4727..5500
                     /locus_tag="U6309_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEN5505818.1"
                     /translation="MKRIHVLFLIGLVVGISSCTKWGKDDEDNNPYKGMTAKQLYTAA
                     QTSLKKEEYATAAKQLEAMESMYPFSDYTESSQMQLIYAYYKDEDYPSAAATAERFIH
                     LYPRAKNVDYAYYMRGLANFQQTRGVFAKMLPMDESWRDPGTQTQALVDFATLIQKFP
                     DSKYKANALQRMIYLRNMFAQHELNVSLYYFKRKMYVAAIERASYLVKNYPQAPSAQK
                     ALVVMYEANKALGLNKAAEDAMAVYKATYHTSNMIRINS"
     gene            complement(5648..6586)
                     /locus_tag="U6309_002388"
     CDS             complement(5648..6586)
                     /locus_tag="U6309_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEN5505819.1"
                     /translation="MWFNNALMFQYKLDGGCDLNLSLTEEALKPCPPHARFTYGWVPA
                     FEQEMVHEVAGSTLICLGKEERILPRGVINKMLKEKIQQIEAAQGRPVKRAEKAQMAE
                     DLEFELLPKSFCVQKKLLAILDSVSQRLIINTSSNNQASQLTSFMRKSIPGIVIEPLN
                     LTENLALRFAEWIHSPATLPDDFQLASDCLLFSLEDEKKRVHCKGYELPAEEILTLLA
                     QGMGTAEISLIWKDRIQFTLTQDFTFKKLKSLDYLIDDFNEIRQLEEEYQRQDAALTL
                     LSGELRELINGLMKIFVNHAEMKSAQIEKIAEDCTI"
     gene            complement(6704..>7029)
                     /locus_tag="U6309_002389"
     CDS             complement(6704..>7029)
                     /locus_tag="U6309_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEN5505820.1"
                     /translation="RYEIVCLDEKICKLVIHYGFMETINIPRSLEKACNKNILPFKFN
                     VDTATFMVEIPNIMASKEKRSLSFYWQEKLFAFLMRNYSANLNIEFYKLPYNRTIAIG
                     TYCIL"
BASE COUNT         2301 a         1244 c         1356 g         2128 t
ORIGIN      
        1 catcatgccc ggtattaaaa aattcaattt aacttatcaa caagtaccta aaatattttc
       61 attgcctgaa agtgtatttt tatccaaaga tgctgagtta attcctgaag aagtattgaa
      121 acaaaccaaa attcaaaaaa aaattgttgc taaaactatt gagcaaatcg ataaagatct
      181 tgaaagaact tgttacgtgg attttgatgg acttactgtt gcgcaattaa aatctgattg
      241 gttaactgtc aatccacaag gtgagttggt gctatcagaa ggtgtttatc catccttttc
      301 tcagggagac aataccggtg cggtaatagg agaaattctt caaaccaaac atttttcgtt
      361 acctttgtca gaagaagatc ctaaagcatt catggaagta acaagaaata caacgagaat
      421 ttgtttttct tataaaaatt ctgctggaca accctcagga ttttccctgg ttgctgctcc
      481 aaatggtgat agtgaaggaa aacataaagg ctggattatt tcagttatcc aaaataccgt
      541 tgggaattac gaggatagga aagtatcagt tcttgcttct ccaaattatg taaggtctga
      601 agctcaatca acagtcgata tggacactcc tgttgctatt gaaagagaat taaagagaat
      661 cattaataat gacaaagtct ccagtttaat aaaaggcatt tttaaggaag atggaacact
      721 caatgcggat tatattcgac aattggcttc acggatcaaa gcgaatcgaa atatagacga
      781 tcgtgaccga aaatctgcac aattcaatat tctcatcgaa ttggcagaac aattggatga
      841 tgaaaaatta agacactata tcaattcaat aaaagctgat atggctgcag atattgagtt
      901 tttcaaagat ggaatttatg agcagcatct aagtgagtta tccaacaaga tttccttatc
      961 attaagtaag atgccttctg taaataaaaa aactactgaa ataggcagaa tattaaataa
     1021 tatttctgag cgttatgcac agattaaaat gattgaggat gcacagagaa agaacccgga
     1081 gttagcccat atttttaagg caatggctca aaaaataaaa tccgatttcc atgatataaa
     1141 atttgatgga acacgtcagg ataatcgaaa tattttgctc ttaagtcaat ttgaggtcgc
     1201 aagtgttgaa caaaagatta gaaactatta tcaaaccaat cctctggtaa aacaatattt
     1261 tatagataaa gataaagcaa atcatcattt acaagttgag ctaagccggt tatctactcc
     1321 caaaaatgag ggttttttca gtagaaataa agattcattt ggatttagta tttttatggg
     1381 tctaatcgta ggagtcagtg cggcattatt tttgactggc gttcttgccc ccatcggagt
     1441 agctctcatt gctgttggag cagccttgat tacaacagga ctggtagggt caatcgatat
     1501 ttatatcaat gagaaagagt acgctaaaga aacatcgcat tattctaatg aggtaaaatg
     1561 cttaaggagt gctcataggg agcatttggt tcgtttagaa aaaaatttac atgagcaaat
     1621 caatttatct gatttcctaa ctcctgataa accggagttt ttatcaggca tcagagtcaa
     1681 taagaaaaaa ccgctgactc cggagagagt ggcccatgct ttatggctac aggataaaag
     1741 tgctcgagat acttcagtag caaaaaatag attttcgata tgggctgcca agcaggaaaa
     1801 tctggatttt ggtatagagg aagtggaaag cgtaatcaaa caaagatatg tcatgaaatc
     1861 ataaaaaata ttgagaattc aatgaagcag acatttatga ctcttggcaa aacatagcaa
     1921 acgaatcagc tgattggata atacagtaag aaaatatgtt aactgatact caaatacaac
     1981 aagctattaa taatcttaat atccttattg cacaccataa tttgccacta tcttgctttt
     2041 cttttaaaaa tgagcaagtt acaaggaact ggcttttcaa atctgaatcg atacatgcta
     2101 ctgctcttag taattattta aagaaaatta tcaatgaagt ttcgagcggc gaagtagata
     2161 agactcatag tttatatgct agtttgttta ttaccttaca aaatttcctt catttacaac
     2221 cgaatcgggt aattgagggc ttgaatttta atttagagaa tagtcagtac ctgaaacgca
     2281 aaaacttgat tatcaagaga ctggaacatt ttcatatcga gaatcaattc agggatgatc
     2341 tgaaacggca tcttcttcct gagattcaat atgatacgta cactgggcat tatacctcac
     2401 cagacgatga taaagcgcat caacatggct cagaagccca gcgaattttc ttctctactc
     2461 aatgggaaag attaaaagct ttatgtaatt ggttattggc taaaatgggt attcatattt
     2521 ttgaaaccgc acgttataaa gaacatgact atttggatag tgagatctat gccgatgatc
     2581 cagcgattga accaggggta agcccacaac aatcagccac tcattactgg attggccatg
     2641 catcaaatct aatgaccata ccagctcatg attcgccatt gcatgtagtc actgatcctg
     2701 ttgaaggtga tttggctcca tttctatacc cgcgtatgac aaaagaaggg agtttaattg
     2761 atggaactgg cagtaaaaaa ttacccaaaa ttgatatagt agtgatttct cacaatcacc
     2821 atgatcatgt aagccagtcc acattgaagc gcctattaaa acagcaaccc aagatggtag
     2881 ttccggaggg tgacgaatca ttgtttttag gcatgggatt taccgatgtt gtagggttga
     2941 aatggtggga acaagcaaag attacagatt tccaagggaa tgaattatta aggataactg
     3001 cagtgcctgc caggcattgg tcgggtcgat cgctgaccga tgctcattgc tcagcattta
     3061 atggctatgt gctgcatgca aatcaattag atggagatat ttattttgct ggggataccg
     3121 cgttaatgga tgacatgtta tcacagccca tttttgaaaa attcaatatc gttacctcta
     3181 ttcaacccgg tggtccagat gagcggcgtg aagatatgga gtctactcat caatcttctg
     3241 cagatgcaat tctcatgcat tttaaaatat tggctgcaca atatcaaaaa atgaaagaga
     3301 agaatgagag tttatctctt gatgagtttt taatggaaaa tcaacatatt aaaactcttt
     3361 ataaccatac tgctactttc aaattaggaa atttacgttt gaaagatacc tattacagtt
     3421 atcaaagaat ggttgctgca tttagggaag atgctcaatg gagagctgat catttaccaa
     3481 agcatgagca aaaggtttac gatcatattc aacttttgat aagcaatatg gttttcaaaa
     3541 ataatcaacg gcttagtaat cggcaaattg tcgatttgat tctgggcaag gttgttattc
     3601 ccaaaattgg tcaaagacaa ccactttatt ttaaagaaaa atcgataaag acagaagcat
     3661 ttcaatatcg taatctgatt actaatcgtc gtgctttact cgaatttgac atgttgctgc
     3721 aagaatccat tgctgagtcg aatgaattct ttaatatcaa acgacttatt ttaaatctgc
     3781 tgcgagccta tcaaaatccc tggcatgcct ttttttcccg aacgcatctt ggagtaggta
     3841 attacgagca agatatccgc aattgccaga gtaacagcga tttactcgct gtattaaacc
     3901 agatggaaag aaacttggga aaaagaaatc cacatggcca tatgcagagt ttgatttatt
     3961 atgctaaatg ggtaatcgat ttttccaata aatatcaaca tgaaacatta atgaaattta
     4021 aagaatattt tgcttgccaa cagataagaa aattggctga tcaagaaatt catcatgctg
     4081 gtcttatagt gggtctcgga gatcgccaag cgaagcaaca gcggtttaaa gtactctcaa
     4141 atgaattggc tgatttgcct gtggatgtta cagaatatag aaaagccatc gataattggc
     4201 ttgccacacg agttaatgat ggacaatcta cccaacaatt gcttagtgaa aatcgctcat
     4261 ctgtttttgc aaaaaaagta acccgtggag aaagcaccgt gctccatatc aaaacaactc
     4321 ttgatctggc agtatcaagt taatagatac aatgacttta gtcttttgta ttaatgggtt
     4381 agatgtcatt tacttacttt ttgtgcttga agaatagaat aacgctaaag tgaaattggt
     4441 tgtctcggga agatataccg tggtaatgtt gtctggcgga gagacaggga ttcgaaccct
     4501 gggaaggttg cccttcaacg gttttcaaga ccgccgcaat cgaccgctct gccatctctc
     4561 cggtcgagaa tactatatga acgcatatca ttttgcaaat gttttttcat agataaataa
     4621 aagaataata ggttgttcgc aaattaactg gtcctaggta taatgtccca aattttccag
     4681 ttgctgtact tataaataca gcagatataa ttgataggtg tgctttatga aacgaattca
     4741 tgtgttattt cttattgggt tagttgttgg aatctcatcg tgtactaaat ggggaaaaga
     4801 tgatgaggat aataatccat ataaaggcat gacagctaag cagctctaca ctgcggcaca
     4861 aacctcttta aaaaaagagg aatatgctac tgctgccaaa cagttggaag ccatggagtc
     4921 tatgtatcct tttagtgact atactgaaag ctctcaaatg cagcttattt atgcctatta
     4981 taaagacgaa gattatcctt ctgctgcagc aacggctgag cgctttattc atctgtatcc
     5041 acgcgcaaaa aacgttgatt atgcctatta tatgagaggt ttggctaact tccagcaaac
     5101 acgaggcgta tttgccaaaa tgcttcctat ggatgaatcc tggagggacc ctggaacaca
     5161 aactcaagca ctggttgact ttgctacttt aattcagaaa tttcctgaca gcaaatataa
     5221 agccaatgct ttgcaacgta tgatttatct acgaaatatg tttgctcagc acgaactcaa
     5281 tgtctctctt tactacttca aacgtaaaat gtatgtggct gccattgaac gggccagtta
     5341 tctagtgaaa aactatcctc aagctcccag cgctcaaaag gctttagttg taatgtacga
     5401 agccaataaa gcattagggc ttaacaaggc agcggaggat gctatggctg tttacaaggc
     5461 tacttatcat acttctaata tgatcagaat taattcttga tgagtacaaa aatttataga
     5521 gtttattctg tcagctgggt tcagcaagtg atgtgcaatc cactcttttt ctcttgaatc
     5581 aagatgagag ggagctctgt tagcaaagga gttccctcga taggttacag gttaaaatga
     5641 tacgacttta tattgtgcaa tcttctgcaa ttttttcaat ttgagccgat ttcatttctg
     5701 catgattaac aaatatcttc attaatccgt taataagctc tcttaactcc ccacttaata
     5761 aggtcaacgc ggcatcttgt ctttgatact cttcttccaa ttgtctgatc tcattaaaat
     5821 cgtcgatcag ataatcaagg cttttcagtt ttttaaaagt aaaatcctgc gtcaaggtga
     5881 actgaatgcg atctttccaa attaatgaga tttctgcagt ccccatacct tgagccaata
     5941 gagtcaggat ttcttcggca ggtaattcgt agcctttgca atggactcgt tttttttcat
     6001 cttcaagaga aaagagtaaa caatccgagg ctaattgaaa gtcatcaggt agagttgctg
     6061 gagaatggat ccattctgcg aatcgcaagg caagattttc ggtaaggttt aatggctcta
     6121 tcacaatacc agggattgat ttacgcataa aagaagtcaa ctgagacgcc tgattgttgc
     6181 ttgaagtatt aataattaat ctctgactga cgctatcaag aatcgctaaa agtttttttt
     6241 gaacacagaa ggatttaggc aatagttcaa attcaagatc ctcagccatt tgagcctttt
     6301 ctgcgcgttt taccggtcga ccctgggctg cttctatttg ctgaattttt tccttgagca
     6361 ttttattaat gactcctcta gggaggattc gctcttcttt acccaggcaa ataagcgtgc
     6421 taccagctac ttcatgcacc atttcctgct caaacgcagg aacccagcca taagtaaagc
     6481 gagcatgggg ggggcagggc ttaagcgctt cttcagtcaa agaaaggttt aaatcacatc
     6541 caccgtctag tttgtattga aacataagtg cattattgaa ccacataatc agtccttaaa
     6601 ataaatttgg gatgttatca cggatagcat tttactgcga tgattagggg ctgttgccat
     6661 ttcgacgcaa gagagcaaac tgtcaacaga ccctggccaa aactcataaa atacaatagg
     6721 tacctattgc tatagttcta ttgtaaggta atttatagaa ttcaatattt aaattcgctg
     6781 agtaattgcg catcaagaaa gcaaacagtt tttcttgcca ataaaaactt agtgatcttt
     6841 tttctttcga agccataata ttgggtattt caaccataaa tgttgctgtg tcaacattga
     6901 acttaaaagg aagtatattt ttattgcaag ctttttccag acttcttggg atattaattg
     6961 tttccataaa gccataatgt attactagtt tacatatttt ttcatcaagg caaacaattt
     7021 catagcgct
//
LOCUS       DASLOE010000151         6994 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.152, whole genome shotgun sequence.
ACCESSION   DASLOE010000151 DASLOE010000000
VERSION     DASLOE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.152"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(174..1556)
                     /gene="mavV"
                     /locus_tag="U6309_002390"
     CDS             complement(174..1556)
                     /gene="mavV"
                     /locus_tag="U6309_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavV"
                     /protein_id="HEN5505821.1"
                     /translation="MKYAVTFCTTDQEVGSNPLWHSCLLLSKMDETSKLLEVVDNWGF
                     YGLPTTNRNSSWLDQLKIKMGLDVDLIGNHGMLRHEELRFLDQGCGLRGVTFELTKDD
                     FELLQKKCLQMATDQETAINEFVETQGIKGKPPEKTRIYPHEQFSRLIYATEKIKAEQ
                     QGREARLKPFELRLSWGLFGPSLALSQNCKTQALSLLSYVLSKEQIERLTEYGEHPTV
                     PRRSGPMESIFLHSTGHLREHRKSSGDIVHYRDLKDPGVKLYWTVPPQELEALSEETI
                     KLFQIDGEYCEEVKAIVRKLQRLEWLLIDSNLPEKYRPYKDELINKVVGCYKAFSFIE
                     PKQEEPKLGGWYGWALSLFSAPRSAEERTLQGKIKKAKSLFNSIYMAIVDNYQIDDNA
                     PMENKNILPYEETGENRFENMVEAVASYLSRKDKINLCKIIGRTYCEDHVELEDNEGK
                     IALSPQPLAT"
     gene            complement(1964..5566)
                     /gene="enhC"
                     /locus_tag="U6309_002391"
     CDS             complement(1964..5566)
                     /gene="enhC"
                     /locus_tag="U6309_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced entry protein EnhC"
                     /protein_id="HEN5505822.1"
                     /translation="MKSLVPWVCVLVASAGQQAFANDAFNAYRLGNYNEAAEPLIIKS
                     GQDAVAEYYLGRLYLYGYGQLKNNRVAMRYFTKSAEKGYLPAVLLMAKYSLLHEKNPE
                     EAARWFKQAAAVGDVSAQMFMAAAYLYGVGVKKNTDIARRYYIDAAKNGNPIAQYTLA
                     EYFLESKHAGNKKLGIIWLNKAAENGDPKALTELGRFYVAGQMVAKDTAKGVELLNKA
                     ASAQYAPAMLELGKLALMQNQYDEAIQWLNKAAKQSTEATLELAHIYLQDKSPVYDPK
                     TGFLLVLKAAQNGSVQAQKELANLYQKGIGVAADEELSKQWTLKAAENEKKNAEPPVL
                     AQAALWLSNDRTDKLEETAYQMNGIFSPWNNPSALRNNSYNQAPKLETISRQDIFKPQ
                     FELTQPNSIPISNYYDALISKQVEFQSNQWAYPTYRLNPFVEALERANSPVVQKLNLP
                     VPYADANYDDSEVGSFDLMDIWTGDWKRTVNYMSVVNYLYFRAILGNAQTQFEIGQLF
                     QYGIGLMQDDASAIIFYENAAEQKHLGAEYNLGILYLKRGKDENDYQQALNWLTDSAF
                     KGNKRAQYVLARILRQGIVGPDGKEYIKANDEQAMAMLYLSAANDYGPAEYELAEYLA
                     RDYNNGLSVDVRKQKIALIRKLYQGAVKNKVAEALLPLAFYNAMDDNRQKQEQAFKVA
                     EEQAETGNENAALLLGMLYDRGIGITADPAKAMYWYQQTGQNPVSQFILGTYITEGKG
                     VAQDKEKGLDLLKQSADSQFSYADFNLAVLKQQSGEDFLPNLIESYKLGNSHAGIVLA
                     DYYLSQSSVPEQMNQAKEIYKGLADKGDQYAQLKLAYMLQKGLGAAPDLAGAQHWYTA
                     SAEQGNPEAQYLLGQFYQLGETGEPDYNLAKYWYQKAAKHLSKADVALGFIYETVDDN
                     YAQALKAYENAAAKGDMLGTYNLALMYEYGKGVPVNYSKALSLFKEASEKGAPEAMSQ
                     LAGMYFYGLGQPRNEQQALVWYKKAASLGNGNALYALGLLSETGVGVKLDFPDALRYY
                     QDASDKGNEKAMLALARMYHYGLGVEKDHKRSASIYQKLAQKQNAYAQYQLGTYYIEG
                     TAGERLPEKGRELLQQASENGSLQARRVLQRLDAQTQGKVSYVEPVLFNNVPKFDGQS
                     ADMIYFDALNEWNHGEEVLSRMILQRLVTQYPNFIPAKRAYEQLNQAKLMNNLG"
     gene            complement(5593..6159)
                     /locus_tag="U6309_002392"
     CDS             complement(5593..6159)
                     /locus_tag="U6309_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase IV"
                     /protein_id="HEN5505823.1"
                     /translation="MNLKSRSSITAIVVILLTSALNISAFAAKSNKAATAEDKAEKKD
                     NGKFPLGCTAVGYQQNLKVLTLLPGKEGALQSMYFIYNTLPETVSLYQMHDKESLYSY
                     WLNHSIRGNSWAVLATGEKEVKFICTLGDGKKSYGKILDCADVIKVCEYVNVKFGLNN
                     KGNFWIVDSNTRNGAVSQVVHYGIIPGV"
     gene            complement(6156..6878)
                     /locus_tag="U6309_002393"
     CDS             complement(6156..6878)
                     /locus_tag="U6309_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEN5505824.1"
                     /translation="MKKILLTTFMMSMVTALNAGHFYGTALCAYPQYECIKVTGGQSW
                     EKLFPDPVQRDIVQRVNRTYNYLWSGREIAVPKNLANINIFDVSPFPLKIQSENEKQI
                     IVDQEKLAWGAYDAQGNLVWWGPISSGSDKCSDSNKVCRTLTGIFRVFSKENVRCTSD
                     VFPIGRGGAKMPYCMYFHKGFALHGSDDIPGVRASHGCVRMFIQDAKWLNENFVELSS
                     ERNNFMGTKVIVRPLNDSENKK"
BASE COUNT         1997 a         1577 c         1130 g         2290 t
ORIGIN      
        1 ataaactaaa tgactgaatt aaaaccattt tttaacgaag agattaggag atttcataaa
       61 tcctttttta tttgatattg attcctaaaa agggttggtc tttatcagtc cctcaacacc
      121 aacccaagct ggaatcacaa aaagctcatg aactggaatc tattccacaa cctctacgtt
      181 gccaaaggtt gtggagataa agcaattttc ccctcattgt cttctagctc gacatgatcc
      241 tcgcaatagg tgcgtccgat aattttacaa agattaattt tatccttcct tgataagtaa
      301 gaagctactg cttccaccat attttcaaat ctgttttcgc cagtttcttc atagggaagg
      361 atatttttat tttccattgg agcattatca tcaatttgat aattatccac aatggccata
      421 taaatgctgt taaataacga ttttgctttt ttaattttgc cttgtaaggt tctttcttcc
      481 gcacttcttg gtgctgaaaa taaagaaagc gcccagccat accaacctcc aagtttaggt
      541 tcttcttgct taggctcaat aaaagaaaaa gctttataac atccaactac cttattgatt
      601 aattcatctt tataaggccg atatttttca ggtaaattgc tatcgattaa tagccactcc
      661 aggcgctgca gttttctgac aattgctttc acttcttcgc aatattcccc atctatttga
      721 aataatttga ttgtctcttc agataatgct tcaagttctt gcggaggaac tgtccaataa
      781 agctttactc caggatcctt taaatcacga tagtgcacaa tgtctcctga agatttccta
      841 tgctcccgta aatgaccagt actatgtaaa aaaattgact ccattggccc actacgtctg
      901 ggcactgtgg gatgttcacc atattctgta agacgttcaa tttgctcctt tgataataca
      961 taagacagca atgataaagc ttgtgtttta cagttttgtg aaagagctaa actaggccca
     1021 aacaatcccc aggacaaacg caactcaaaa ggttttaaac gagcttcccg cccttgttgt
     1081 tccgctttaa ttttttcagt cgcataaatc aatcttgaaa attgttcgtg aggataaatt
     1141 ctggtttttt caggtggttt ccccttgatg ccctgcgttt cgacaaactc atttatcgct
     1201 gtttcctgat cagttgccat ctgcaaacac tttttttgta ataactcaaa atcgtctttg
     1261 gttaactcaa aggtaacccc gcgcaaacca catccctgat cgagaaatct gagctcttca
     1321 tgccgcaaca ttccatgatt gccaatcaaa tcaacatcca gccccatttt tattttcaat
     1381 tgatctaacc aagaactatt acgattggtt gtcggtaatc cataaaatcc ccaattatca
     1441 acaacctcca gcaatttact ggtttcgtcc atttttgaaa gcaatagaca actgtgccac
     1501 agtggattac tccctacctc ctgatctgta gtacaaaatg taacagcata tttcatatca
     1561 caaccctcat cgatttgtat ctaattattt ggccttgatt aaatagatct tttgcccaag
     1621 ccgattcttt cattttattg ctgctttgca aacatttctg cattgctcat taactgcatt
     1681 ataaactccg ctcctcaaaa cgtttgtacc ttgccctaaa aaaataatcg gcttaggcat
     1741 aaagcctaac taacaattta aatctctaaa agatcatttt taaaattaca ataaggcatg
     1801 gctacacttt cgtaaatagc caagatgagc acaatccttc atgtgtttaa tataatcgag
     1861 gcaacaaatg caggctgggc cttgtggccc agagaaatcg gataattgat tagcaaaatt
     1921 gaaacatcat ctgttaaata gctcaaagag ctgagcaatg atcttaaccc aagttattca
     1981 ttaatttggc ttgattcaac tgctcataag ctcgctttgc agggataaaa ttgggatatt
     2041 gcgtcaccaa acgttgcaag atcattctgg acaaaacctc ctccccatga ttccattcat
     2101 ttaatgcatc aaaataaatc atatcagcgc tttggccatc aaacttggga acattattaa
     2161 ataacacagg ctcgacataa cttactttac cctgggtttg tgcatctaat ctttgcaata
     2221 ctctacgggc ctgtaaactt ccgttttcac tcgcttgttg taatagctca cggccttttt
     2281 cgggtagtcg ttcgcctgcc gttccctcaa tataataagt acctaattga tattgagcat
     2341 aagcattttg cttttgtgcc aatttttgat aaatactcgc tgatctttta tgatcctttt
     2401 cgacaccaag accataatga tacattcttg ccaaagccaa cattgctttt tcattgcctt
     2461 tatcagatgc atcctggtaa taccgtaaag catctggaaa atccaattta acaccaacac
     2521 cagtttcaga taataaaccg agggcataca aagcattccc attacctaag cttgcagcct
     2581 ttttatacca gaccaaagct tgttgttcgt ttcttggttg ccccaaacca taaaaataca
     2641 ttccagccaa ttgactcatc gcttcaggag cacctttctc tgatgcttct ttaaacaaag
     2701 ataacgcttt ggagtaattg acgggcacgc ccttgccata ctcatacatt aaagccaaat
     2761 tataagtgcc tagcatatct ccttttgctg cagcattttc ataggctttt agtgcttgag
     2821 cataattatc atctaccgtt tcataaataa aacctaatgc tacatctgct ttagataaat
     2881 gtttcgctgc tttttgatac caatatttgg ccaaattata atcaggttct cctgtttcac
     2941 ctagttgata aaattggcct aataagtatt gtgcctcagg attgccttgt tctgccgaag
     3001 cagtatacca atgttgcgcg ccagcaagat ccggtgcggc acccaatcct ttttgtagca
     3061 tataagccaa ttttaattgt gcatattgat cacctttatc agctaagccc ttataaattt
     3121 ccttagcttg attcatttgt tcaggaacac tgctttgaga caaataataa tctgccaata
     3181 ctatccccgc atgactattc cctaatttat aagattcaat taaattgggt aaaaagtctt
     3241 ccccggattg ttgcttgagt actgccagat taaagtcggc ataggaaaat tgggagtctg
     3301 ctgattgctt cagcaaatcc aatccttttt ccttatcctg tgcgacccct tttccttctg
     3361 ttatgtaagt acccaatata aactgactta cgggattttg tccggtttgt tgataccagt
     3421 acatggcctt ggctggatca gctgttattc ctataccacg gtcatagagc attccgagta
     3481 aaagagcggc attctcatta cctgtttcag cttgctcttc ggctacttta aatgcttgtt
     3541 cctgcttttg cctgttatca tccatcgcat tataaaatgc caatggcaac aatgcctctg
     3601 ccactttatt cttcacagca ccttggtata actttcttat taaggcaatt ttttgttttc
     3661 tgacatctac acttaaacca ttgttgtagt ctcttgccaa atattctgcc aactcatatt
     3721 ctgccggccc ataatcattg gcagctgaaa gataaagcat ggccatagct tgctcatcat
     3781 ttgctttaat atactctttt ccatcaggac caacgatccc ctggcgcaat atccttgcca
     3841 atacgtattg tgctctctta ttccctttga atgcggaatc agtcagccaa tttaatgctt
     3901 gttggtaatc attttcgtct ttaccgcgtt tcagataaag tattcccaaa ttatattcag
     3961 cacctaagtg cttttgttct gcggcattct cataaaaaat aatagccgaa gcatcatctt
     4021 gcattaaacc aatcccatat tgaaacaatt gaccaatttc aaattgagtt tgggcatttc
     4081 caagtattgc cctgaaatac aaataattga ccacagacat ataattgaca gtccgtttcc
     4141 aatctcctgt ccaaatgtcc atcaaatcaa aactgccaac ttcgctatca tcataattcg
     4201 cgtcggcata aggaactggc aagttgagct tttgtacaac aggactattg gccctctcca
     4261 gagcttctac gaatgggtta agtctgtaag ttggataagc ccattgattt gattgaaact
     4321 ccacttgttt actgattaaa gcatcatagt aattgcttat aggaatacta ttaggctgag
     4381 tcaactcaaa ttgtggtttg aaaatatctt gacgagagat ggtttcaagc ttaggtgctt
     4441 gattatagct attatttctc aaggcagacg gattattcca tgggctaaat ataccattca
     4501 tctggtaagc cgtctcttcc aatttatccg ttctgtcatt acttagccat aatgcagctt
     4561 gggcaagaac aggaggctct gcatttttct tctcattttc tgctgcttta agagtccatt
     4621 gtttagacaa ttcctcatca gccgctacgc caatcccttt ttgatataaa ttggctaatt
     4681 ctttttgggc ttgaacagaa ccattctgag ctgcttttag taccaataaa aaacctgttt
     4741 ttggatcata aactggactt ttatcttgta aataaatatg agctaattca agagtagctt
     4801 ctgtactctg tttcgcggcc ttattaagcc attgaatagc ctcatcgtat tgattttgca
     4861 ttaaagccaa tttacccaac tcaagcatgg ctggggcata ttgtgctgag gctgctttat
     4921 ttaataattc gacgcccttg gcagtatcct tggcaaccat ttgtccggcc acataaaatc
     4981 tgcccaattc tgttaaagct tttggatctc cattttctgc tgctttattc agccaaatga
     5041 tccctaattt tttattgccc gcatgcttac tctctaagaa atactctgct aaggtgtatt
     5101 gagcaatagg attaccattt tttgctgcat caatataata acgtctcgcg atatctgtat
     5161 ttttcttgac gccaactcca tataaataag ctgccgccat aaacatttgt gcgcttacat
     5221 ctcccaccgc agctgcttgc ttaaaccaac gagcagcttc ttccggattt ttttcatgga
     5281 gcaaactgta tttggccatg agcaggacag caggtaaata gcctttttcc gctgatttgg
     5341 taaagtagcg catcgcaaca cgattgtttt ttaactgccc atatccatag agataaagtc
     5401 gccctaaata gtattcggca acagcatcct gacctgattt aatgattagg ggctcagctg
     5461 cttcattata attgcctaat ctataagcat tgaacgcatc attggcaaaa gcctgttggc
     5521 cagctgacgc gactaaaaca cacacccaag gtactagtga tttcatgaag attcccctct
     5581 tttcatggcc atttatacac cgggtataat gccataatgc accacttgac taactgcccc
     5641 gtttctcgta ttgctgtcta ctatccaaaa attaccttta ttattcaaac caaatttgac
     5701 attaacatat tcgcatactt taatcacatc agcacaatcc agtatttttc cataggactt
     5761 tttgccatca cccaaggtac agatgaactt cacttccttt tcacctgtgg ccaaaacagc
     5821 ccaggaattc cctctgatag agtgatttag ccaataggaa tataaacttt ccttatcatg
     5881 catctgatat aagctaacgg tttctggcaa tgtattataa ataaaataca tcgattgcaa
     5941 ggccccttcc ttcccaggaa gaagagtcaa tacctttaaa ttttgctgat agccaacagc
     6001 agtacaaccg agaggaaatt ttccgttatc ttttttctct gccttatctt cggcggttgc
     6061 tgctttattg gatttggcgg caaatgccga tatatttagt gcactagtta atagaatcac
     6121 aacgattgca gttatcgagc ttcggctttt taggttcatt ttttattctc actatcattt
     6181 aaaggacgta caatcacctt agtccccata aaattgttac gctcactgga caactctaca
     6241 aaattttcgt ttaaccactt ggcatcctgg ataaacatac gcacacaacc atgactagcc
     6301 cttactcccg ggatatcatc agaaccatgt aaagcaaacc ctttatgaaa atacatgcaa
     6361 taaggcattt tggcaccgcc tctgcctata ggaaaaacat ctgaagtaca acgaacattt
     6421 tccttgctga atacacggaa aattcctgtc aaagtacgac agactttatt ggaatcagag
     6481 catttatcac ttccagaaga aataggtccc caccatacta aattgccctg agcatcatag
     6541 gcgccccaag ccagcttctc ttgatccaca ataatttgtt tttcattttc actctggatt
     6601 ttaagtggga aaggagaaac atcaaaaata ttgatgttgg ctaaattctt tggtacagcg
     6661 atttctctac ctgaccaaag gtagttataa gtacggttga ctctttgcac aatgtctctt
     6721 tggacaggat cagggaataa tttttcccaa ctttgtcccc cggttacctt aatacattca
     6781 tattgaggat aagcacataa tgccgttcca taaaaatgcc cagcgttaag tgcagtcacc
     6841 attgacatca tgaacgttgt taatagtatt tttttcatgg tgccgccccc ttatgcctga
     6901 tttttataat tttatgcaaa atttgtgcta aaaatgaaat gccactcttt ttatcttttt
     6961 tcggttattt ttaatttaag tttagaccca taca
//
LOCUS       DASLOE010000152         6796 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.153, whole genome shotgun sequence.
ACCESSION   DASLOE010000152 DASLOE010000000
VERSION     DASLOE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.153"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..564)
                     /locus_tag="U6309_002394"
     CDS             complement(<1..564)
                     /locus_tag="U6309_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HEN5505825.1"
                     /translation="MTYSFSKPVSWVFLFTAVIYLVSLSFIQYPATTVLKPIPIVCLI
                     VGVFRTFLPSLAKIFLILALVFSLAGDVVLTLPVALQLELGIGCFLLAHCFYIALFLK
                     SFQFNKLHFFYYILILLLMAFIAFTIIPYLGDLLIPVMIYFCVLMLMLFSAFQVKKET
                     LTIGSGALFFLISDLTLALNLFVYTQAD"
     gene            complement(644..1144)
                     /locus_tag="U6309_002395"
     CDS             complement(644..1144)
                     /locus_tag="U6309_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505826.1"
                     /translation="MTKEKSESGNSPSNSEEHGTLGPDVYENARKLNIQLATRGPAIL
                     YALGKASEQVTNGNVVTAVGGMVVGAIGFFGNRFYRNKGDDSAYTYMGQIVGAVQFTA
                     GFTLLALQAFEAFTDTGETSPLLNVVKLSGVALTLTESALILINSYLYGDGSAKTTSV
                     TSPKID"
     gene            1636..2496
                     /locus_tag="U6309_002396"
     CDS             1636..2496
                     /locus_tag="U6309_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN5505827.1"
                     /translation="MLKRLLGVASLFLILNHQCFAEDHEIAITIDDLPFVGSGTSTPG
                     NLKRTQERFMAIVNTLVDNQVPATGFAIGGAIAKNEWELLEIFRNQGFSIGNHTYKHR
                     SLNSMTAENYIADIEKADTVLSPVMTEPKYFRYPYLAEGSGEKKQKVHEWLAAHQYTI
                     APVTIDSKDYEFNAQFYRIPYRQRPQRLAQFKKRYLAFIWQQTLRAEKKVKKAEGQPV
                     KHILLIHANLINSLCLADIIEMYRSNGYKFITLQEALKGNTATPINDSSPTEALKSET
                     EKKEINEPQS"
     gene            complement(2613..3809)
                     /locus_tag="U6309_002397"
     CDS             complement(2613..3809)
                     /locus_tag="U6309_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEN5505828.1"
                     /translation="MKSKLIYLTIALAAIILSAAVWWWLQSPKPTFKQVSFKHLPGWE
                     NAQLKKSLETFQTSCRAFLKQNPERVVGTEQIDLQAKDWHPACRAALKINPVDEQSAK
                     LFFEKWFAPVEFYDDKPVKGLFTGYYMPLIKGSYTKSEEFSVPIYELPSNLVTVDLGL
                     FLPNLKNKKIIGRIAGNKVVPFYTREEINKGAIKNTARVLVWIHSPVDRLFLEIQGSG
                     IIELEDGKRIFIGYDGQNGAPYTAIAGVLIKKGIMTKHNASMQRIKKYLEAHPKQMDK
                     IINQNKSFVFFRKLSLEAALGSQGVALTPGYSLAIDKQWIPMGAPLWLNTTRPDSKNP
                     DVNKPMQRLMIAQDTGGAIRGKVRGDVFWGGGDKATLIAGHMKNEGHYWLLLPAHALS
                     RLAQLK"
     gene            complement(3919..4476)
                     /locus_tag="U6309_002398"
     CDS             complement(3919..4476)
                     /locus_tag="U6309_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505829.1"
                     /translation="MKKTDSGFILLMTLCIIFLMSLLVLASLQQIFLQYKALNSQETF
                     HQNFYQLEHWMMRLARSPLLYAGMDCYVQKDYGANKIANELVNNKGCLLILNQKKYRY
                     FIEDLNEFSCLVLNKDGQKYASHHFRFTVLQDEEHGEFSIMQLRFIKLGSNLSSCPEE
                     EIQMKEGVSSWRYLPDYKNFEILTG"
     gene            complement(4473..5279)
                     /locus_tag="U6309_002399"
     CDS             complement(4473..5279)
                     /locus_tag="U6309_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505830.1"
                     /translation="MLRGLKKASQLFAPMIWVLIEMGRQTGFSLSEMLISLFLASIIT
                     ITLTQFYIRSKPQYLKAQERLEINFDLLWIGDLLSDSIRKAGFTPCVSVEKLKTFDSR
                     HAERKIINFQIENMPQVQLRINRMSDVYTEVANFISPTQIVIEESVISNIKHPLIISD
                     CRQGEVHTVSKIDKLSNGYQITLKKPLQFRYDEIAYLGEWLEETWFTKQNEKGEKTLH
                     YKLFQTEELSPFIHEFYVTRRLINQRSVLDFSIGSNKETLRKISVSVRNL"
     gene            complement(5218..5532)
                     /locus_tag="U6309_002400"
     CDS             complement(5218..5532)
                     /locus_tag="U6309_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEN5505831.1"
                     /translation="MSKHKGFTLMEVLVSLMLMTTVVLMLVEQQLKTRDLINHLIWRI
                     QGSQYLDQVDEALYISENRLPLPPSNYHFDIRRSQKKIILNVTWFKKSQSIIRTYDLG
                     AN"
     gene            5692..>6796
                     /locus_tag="U6309_002401"
     CDS             5692..>6796
                     /locus_tag="U6309_002401"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEN5505832.1"
                     /translation="MSIDFQQLPHAGIRSLIPYVPGKSIEELAKEKGITDIIKLASNE
                     NPLGCSPLALSAIQTMSSHYIATYPSPWNHPLMSKLASYLKVKPEQLFLSNGSDYLFN
                     ILLNCFALHTDRHILTHDYAFSTYAIQANSLQIPINSVPIGHNWEVNVTDIVNACNQQ
                     TGIIFIANPNNPTGVLIQQEEIKYLLEQIPKSTLLVLDEAYYEFAASQLTVNSLDWLE
                     EHPNLVVTRTFSKIYGMAGLRLGYAIANPSIINILKRVQLPFIVNQVALAAAYAAIDD
                     DDFIQSSLKMNNEGMSQLQAGFNELNIKYLPSSCNFLTFDCAEDSMALYNYLLDNGII
                     VRPLHAYKMNNFIRVTIGTKEQNSRFLTALKNFY"
BASE COUNT         2206 a         1332 c         1119 g         2139 t
ORIGIN      
        1 atcagcttgg gtataaacaa acaagttaag tgccagggtt aaatcagaaa ttaaaaagaa
       61 caaagcgcca ctcccaatcg ttaaagtttc cttttttact tggaaagcac taaataacat
      121 aagcatcaga acgcagaagt aaatcatcac tggaattaat aaatcaccaa gatacgggat
      181 tatggtaaat gctataaacg ccattaatag aagaatgaga atataataga aaaaatgtaa
      241 tttattaaat tgaaatgatt ttaaaaacaa agcgatataa aaacaatgcg ctaacaagaa
      301 gcaacctatc cctaattcta attgtaatgc aacaggtaaa gtcaatacca catcccctgc
      361 caaagaaaat acaagtgcca agatcaggaa aattttagcc aatgagggga gaaaggttct
      421 gaatacccca acaattaaac aaactattgg aataggtttt aaaacagtag tagcaggata
      481 ttggataaac gaaagactga ccaaataaat gactgcggta aacaaaaaca cccaagacac
      541 tggtttggaa aaagaataag tcataaatag tcccttattg taaaatcaaa gtcattgatt
      601 tgagctgcct tatacctcaa ccctactagc cttttcattg tattcaatca atcttagggc
      661 ttgttactga tgttgttttg gctgatccat ccccatataa atacgagttg attaaaatta
      721 aagcgctctc ggttaatgtt aacgcaaccc cgcttagttt aacaacattg agtaagggac
      781 ttgtctctcc agtatctgta aacgcctcaa atgcctgtaa tgccaacagt gtgaatccgg
      841 cagtaaattg caccgcacca acaatttgtc ccatataagt atacgcggaa tcatcaccct
      901 tgtttcgata aaaacgatta ccaaaaaaac ctatcgcccc aacaaccatg ccaccaaccg
      961 ccgttacaac atttccatta gttacttgct cactggcttt acctaatgca tacagtatgg
     1021 caggccctct tgttgccaat tgaatattta gctttcttgc attctcataa acatctggac
     1081 ctaaagtacc atgttcttct gaatttgatg gtgaatttcc tgattcagat ttttcttttg
     1141 tcattatcaa ctcctgttta acaaaattcc gggtaagaca gtatcctttc aatttctcat
     1201 attatatcca cggccttgcc ttattgtttg acataatcag ccctaatttc aggtgagtta
     1261 tggataagat cagccgttat cttgaatcgc tcaggctgag gaggcattta tgccatctcg
     1321 aagccttgta tggaatttaa tatttcatgc ttaggctttg agagggtgct tacacatctc
     1381 cctgccccaa acggaccctg gatatcgagt gctccttata taaaccttat gcatagctca
     1441 cgttaattta agtagttctc tatttgtcac tttaataaat tatccaatag ccgaaaaatc
     1501 gaagtattta ttgtacctaa accctctcaa aatacataca tatttccaaa caggtggaaa
     1561 tttaatttat cgtctatagt ttaattaaat gcctaaaata ataagaattt ttccaaattg
     1621 aaaagagggg aaaatatgtt aaaacgtcta ctcggtgtag catcattatt tttaatattg
     1681 aaccaccaat gcttcgctga agatcatgaa attgccatta ctattgacga tttacctttt
     1741 gtgggctctg gaacaagtac tccaggaaat ctgaagcgaa cccaggaaag atttatggcc
     1801 atagtgaaca ctttggttga caatcaggtt cctgccacag gttttgccat aggaggggct
     1861 atagcgaaaa atgaatggga gcttctggaa atttttcgta atcaaggttt ttcaattggc
     1921 aatcatacct ataagcatcg aagtttaaat tcaatgactg cagaaaatta tattgctgat
     1981 atcgaaaaag cggataccgt tctatcccct gttatgactg agccaaaata tttccgttat
     2041 ccttatcttg ctgaaggcag tggagaaaag aagcaaaaag tgcatgagtg gctggctgcc
     2101 catcaataca cgattgctcc tgtcactata gatagcaaag attatgaatt caatgcgcaa
     2161 ttttatagaa taccatatag acaaagacca caacgtttag ctcaatttaa aaaacgctat
     2221 cttgctttta tctggcaaca gaccctgaga gcagaaaaaa aagtcaagaa agcagaagga
     2281 caacctgtta aacatatttt actcatccat gcgaatctga ttaatagttt gtgtttggct
     2341 gatatcattg aaatgtatag aagcaacggt tataaattca ttactctaca agaggctcta
     2401 aaagggaata cagctactcc aattaatgat tcatcaccaa ctgaagcttt gaaatcggaa
     2461 accgaaaaga aagaaattaa tgagcctcaa tcttaaaaaa tatagagtta tttcttgaaa
     2521 caaatgattt acaatgctat cggggattca ttgtcaccgg actggtgaat cccaatagtc
     2581 aaataaaaaa ttgcaaagac aggacttaca ccctacttca actgtgccaa gcgcgataat
     2641 gcatgagccg gcagaagaag ccaataatgc ccctcgttct tcatatggcc agcaatcaaa
     2701 gtggctttgt caccaccgcc ccaaaataca tcacctctta ctttaccacg aatagcccca
     2761 ccagtgtctt gagctatcat caaacgttgc ataggtttat tgacatcagg atttttacta
     2821 tctggtcttg tagtgtttaa ccacagaggc gctcccattg gtatccattg tttatcaata
     2881 gcaagagaat aaccgggtgt taatgccaca ccctgtgaac ccaaagccgc ttctaatgac
     2941 aacttgcgga aaaaaacaaa tgatttattt tgattgatta ttttatccat ttgcttggga
     3001 tgagcttcca gatacttttt aatcctttgc atggaagcat tgtgcttggt cataataccc
     3061 tttttaatta atactccagc aatggcagta tagggggcac cattttgacc gtcatagcct
     3121 ataaaaattc gtttgccatc ttcaagctca atgattcctg agccctgaat ttctaaaaat
     3181 aacctgtcca cagggctatg gatccacacc aaaacacgtg ctgtgttttt aattgctcct
     3241 ttgttaattt cctctcgagt ataaaatggt acaactttat tacccgcaat tcgtccaata
     3301 attttcttat tttttaaatt aggaagaaat aaacccaagt caacagtcac taagttactg
     3361 ggtaattcat aaataggaac actaaattcc tctgatttgg tataactgcc tttaattaaa
     3421 ggcatataat agcccgtaaa taaacctttc actggtttat catcatagaa ttccacaggg
     3481 gcaaaccact tctcaaaaaa taattttgct gattgttcat caaccggatt tatttttaaa
     3541 gcagcacgac aggctggatg ccaatctttt gcctgtaaat caatctgctc tgtgccaaca
     3601 acacgttcag gattttgttt tagaaaggct cgacaagatg tttgaaaagt ttctaacgat
     3661 tttttaagtt gtgcattctc ccacccaggt aaatgtttaa agcttacctg cttgaaagtt
     3721 ggcttgggag attgcaacca ccaccatact gctgcgctta agataattgc agccaaggct
     3781 attgtcagat aaattaattt cgatttcata ggaggtcaat ttttacacgt gatggagaaa
     3841 aatgcaacta aaaattattg ctattagtcg atcttactac aaaaaacatc cggttataat
     3901 agcttttttt caataatttc aaccagttaa tatttcaaaa tttttataat ctggcaagta
     3961 tctccagctg cttacccctt ccttcatctg tatttcctcc tctggacaag aggacaaatt
     4021 gctacccaat ttgataaacc ttaattgcat gatagaaaat tcgccatgct cctcgtcttg
     4081 taatacggtg aatctaaaat ggtgagaagc atatttttgc ccatccttat taagaaccaa
     4141 acaagaaaat tcgttcaggt cctcaataaa atatctgtat tttttctgat ttaatatgag
     4201 caaacatcct ttattgttaa ctaactcgtt tgctatttta ttagctccat aatccttttg
     4261 cacataacaa tccatacctg catacaataa cggtgaacga gcgagtcgca tcatccaatg
     4321 ttccaactga tagaaatttt gatgaaatgt ctcctgtgaa ttgagtgctt tgtattgcaa
     4381 gaatatctgt tggaggcttg ccaaaaccaa taagctcatc aagaaaatta tacaaagcgt
     4441 cattaaaaga atgaaaccgg aatcggtttt tttcataaat ttcgtaccga aacactgatt
     4501 ttacgtagcg tttccttatt actaccaatt gaaaaatcca atactgacct ctgattgata
     4561 agacgtctgg taacataaaa ttcatgaatg aatggactta attcttctgt ttgaaacagt
     4621 ttgtaatgta atgtcttttc acccttttca ttttgtttag taaaccatgt ttcttcaagc
     4681 cactctccca agtaagcaat ttcgtcgtac cggaactgca gtggtttttt taaagtaatt
     4741 tgatatccat tgcttagttt atctattttt gaaactgtat gtacttctcc ttgccggcaa
     4801 tcagaaataa ttaatgggtg ttttatattt gatattactg actcttcaat cactatttgt
     4861 gttgggctta taaaattcgc cacttcggta taaacatcac tcattctgtt aatacgaagt
     4921 tgaacttgtg gcatattttc aatttgaaaa ttaattattt tcctctctgc atgtcgagaa
     4981 tcaaaagttt ttaatttttc gacacttacg catggagtaa atccagcctt tctaatactg
     5041 tcgcttagta aatcaccaat ccacagcaaa tcaaaattaa tttctaaccg ttcttgtgcc
     5101 tttaggtatt gaggcttgct tcttatataa aactgagtaa gagttatcgt tataatactt
     5161 gccagaaaaa gactaattag catctcagat aaactaaatc ctgtttgtct tcccatttca
     5221 attagcaccc agatcatagg tgcgaataat tgactggctt tttttaaacc acgtaacatt
     5281 caaaatgatt ttcttttgag atcgcctgat atcaaaatga taatttgaag gcggcagagg
     5341 gagtctattc tcactaatat ataatgcttc atctacttga tctagatatt gggaaccttg
     5401 gattcgccag ataagatgat tgattagatc tctggttttc aactgttgtt ccactaacat
     5461 cagaactacc gttgtcatta gcattaatga cacaagtact tccattagag taaaaccctt
     5521 atgcttgctc ataataaatc tcttcctgag gttggactgc ccatgaaggt taactgttcg
     5581 tttatataaa ttcaatataa cgatttataa attctaaaaa ataaattcat tcgtgacagc
     5641 tggcatatgc ttaggtaata taaaaacatt tttctgtcta ggaatagagt tatgtctatt
     5701 gattttcaac aattacctca tgccggaata cgttctttaa ttccttatgt accaggtaaa
     5761 tccatcgaag aattggcaaa agaaaaggga ataactgata ttatcaagct ggctagtaat
     5821 gaaaacccat tgggatgcag cccattagcc ctttccgcta ttcagactat gtcatcgcat
     5881 tacatagcaa cctacccatc gccctggaac catcctttaa tgtccaaact tgcaagttac
     5941 ttaaaagtaa aacctgagca attgtttcta agcaatgggt ctgattacct gtttaatatt
     6001 ttattaaatt gtttcgctct tcataccgac agacatatcc ttactcatga ttacgctttt
     6061 agcacttatg ccatccaagc caactccctg caaataccaa taaatagtgt gcctattggc
     6121 cacaactggg aggttaatgt cactgatatc gttaatgctt gcaatcaaca aacaggaatt
     6181 atttttatag ctaacccaaa taatccaact ggggtgctaa tccaacaaga agaaattaaa
     6241 tatttattgg aacaaattcc gaaaagtact ttgttagttt tagatgaagc ctattatgaa
     6301 tttgctgcta gccaattgac tgtcaatagt ttggattggc ttgaagaaca tcctaatttg
     6361 gttgttactc gtacgttttc aaaaatctat ggtatggcag gtttgcgttt aggttatgct
     6421 attgccaacc catccattat taatatttta aaacgagtac aattgccatt tatagtcaat
     6481 caagttgctc ttgcagcagc ctatgctgcc atcgatgatg atgacttcat ccagtctagc
     6541 ctcaaaatga ataatgaagg gatgtcgcag ctacaggcag gctttaatga attaaacata
     6601 aaatacttac cctcttcctg taatttcctt acttttgatt gcgcagagga tagtatggct
     6661 ctttataatt atctattgga taatggcata atagtaaggc ctctgcatgc ttataagatg
     6721 aataatttca tcagggttac tattgggacc aaagaacaaa attccagatt tctaacagca
     6781 ctgaaaaatt tttatt
//
LOCUS       DASLOE010000153         6788 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.154, whole genome shotgun sequence.
ACCESSION   DASLOE010000153 DASLOE010000000
VERSION     DASLOE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.154"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(11..568)
                     /gene="frr"
                     /locus_tag="U6309_002402"
     CDS             complement(11..568)
                     /gene="frr"
                     /locus_tag="U6309_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEN5505833.1"
                     /translation="MINEIKQDSEKRMKKTIEALHTDMSKIRTGRANASLLDHVMVDY
                     YGSPTPLSQVANITTSDSRTILVTPWEKSMVAAIEKAILNSDLGLNPATAGTAIRVPM
                     PPLTEERRKELIKVVRHEGEQGRVSIRNIRRDANNQLKELVKEKAISEDDERRAAEAI
                     QKLTDRYISEVDAVLAEKEKDLMEI"
     gene            complement(558..1301)
                     /gene="pyrH"
                     /locus_tag="U6309_002403"
     CDS             complement(558..1301)
                     /gene="pyrH"
                     /locus_tag="U6309_002403"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEN5505834.1"
                     /translation="MMNESQPKLKYKRILLKFSGEALMGKSQFGIDPSVLDSLARDIA
                     ELIHMGVEVGLVLGGGNLFRGKALSQAGVGRVTGDHMGMLATVMNALALRDALERIDL
                     PARIMSAIPMLGVVDPYHRRKAITHLRNGQVVIFAAGTGNPFFTTDTAACLRAIEIGA
                     DIVLKATKVDGVYSADPLKNSDAKRYDYLTYKEVLTKGLEVMDSTAICLCQDQGMPLQ
                     VFDMAAPKALKRIVTGERVGTIVGANHDQ"
     gene            complement(1298..2176)
                     /locus_tag="U6309_002404"
     CDS             complement(1298..2176)
                     /locus_tag="U6309_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEN5505835.1"
                     /translation="MSTISAALVMQLRERTGAGMMECKKFLIATNGDIEQAIIEMRKA
                     GQAKADKKADRVAAEGIIVIARSSDERTAVMLEINSETDFVARDENFTNFANAVADAA
                     LTNLPKNIEDLSNQALSSGTTVEQARQELVAKIGENIKLRRLERMHCDGVIGYYLHGS
                     RIGVMVALKNGSEALAKDIAMHVAASKPMVVSRDQVPAEAIENEREIFTAQAKESGKP
                     QEIIDKMIDGRINKFIDEVSLLGQPYVKDPNIKVGQLLKEKNAEVISFVRYEVGEGIE
                     KKEDNFVEEVMAQVRT"
     gene            complement(2242..3006)
                     /gene="rpsB"
                     /locus_tag="U6309_002405"
     CDS             complement(2242..3006)
                     /gene="rpsB"
                     /locus_tag="U6309_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEN5505836.1"
                     /translation="MNNVSMRELLEAGAHFGHRTRFWNPKMSEYIFGSRNKIHIINLE
                     KTLPMLNDVTNYVSRLAANKAKILFVGTKRAAQDSIREHAKRCGMPYVDHRWLGGMLT
                     NYKTVRQSIFRLKELKEMKEKGLFNDMIKKEALMLTRELEKLERSLGGIENMGGLPDA
                     LFVVDVGFEHIAVEEARRLRIPVIGVVDTNNSPDNIDYVIPGNDDSMRAVDIYVRCVA
                     DAILDGKNSNTVGRVSSDSEFVEVTSNSNEEEKSGE"
     gene            3338..3748
                     /locus_tag="U6309_002406"
     CDS             3338..3748
                     /locus_tag="U6309_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEN5505837.1"
                     /translation="MSKKSICLATLALIFTFGQPVFANSSEATQTKPCHCHDHKKILS
                     QLNLTQEQKTKIKTIKIQAQKSIKENYRQLKAIKEQINALIANEKLDEAKLDSLINQR
                     NKIAGTMLKNRVMMKNQIYNQLTNEQKSQYKKHM"
     gene            4258..4716
                     /locus_tag="U6309_002407"
     CDS             4258..4716
                     /locus_tag="U6309_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1716 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505838.1"
                     /translation="MRGHMIFLSIPKGMEFKQITEKDNTNDYFVDPNGKLPRIDIQAL
                     VKDALQYNKGRKKEIALSDFTIYRHKPPYKDELFLQYNPDHNGKYFTKESVSLVNGKE
                     FIRNKAPATSYGTFWFQKVQLSESRMDEVLAKRSEQRENRRHTGNSPNPT"
     gene            complement(4758..6389)
                     /locus_tag="U6309_002408"
     CDS             complement(4758..6389)
                     /locus_tag="U6309_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5505839.1"
                     /translation="MNIVAQNPKLAQKIRETSNYYTKEVALIASNRQSDRDDKRNARS
                     NGNGSCFIQIQKLCTDLGMAFDPFLLADAYNLVPAGTSLHEATNPANGGKVQNGWLHD
                     KSKTSLVYAHMQRASSLEAREKIDYFFYDDREDLLEGLHQFFSEYPELIPSNVTMHLV
                     KYACNRENVHTLDNYKIPVQGKGFTNPDYAKTIKAFGDKAIEYARDIYDTEGKEINAA
                     GVRQAKYNCNFEFEANKLQPMKFVNNFNSPAELIKAHEQAVEKIKLDAFELYKAKINA
                     ITTLSNEQKAFLIRAKAQQWNVPCDEGQWSNPIFTLQAIMAEKSDRFFLSQYAFTAKM
                     LITNYQEARENRKDGSRFWEQVNVFIKSVNALKDPMRNAQSIFEAFKKNSYSVKAVDG
                     FVKKVVEVMNALMDKYPQRSTTEMVACMRILESNLQKMVNFFSLVDRLITIYRGCTTA
                     GPNFNKILTVVREALEEMKMITDSGDNYSTTLIDLDKVITAANNRLEGLTYAKEYSTK
                     DFYSPESYKVVGAYLTMQSKFTHQLDNKASAAFAI"
BASE COUNT         2100 a         1427 c         1101 g         2160 t
ORIGIN      
        1 catctacacc ttaaatttcc attaaatctt tttctttctc ggctaatact gcatcaactt
       61 cagaaatata tctatcagtc agcttttgga tcgcttctgc tgcacgtcgt tcatcgtctt
      121 cagaaattgc tttttcttta acaagttcct ttagctgatt attggcatcc cttctgatat
      181 ttcgaattga aactcttcct tgttcccctt catggcgaac aactttaatc aattcctttc
      241 tgcgttcctc tgttaaaggt ggcatcggaa ctcgaatggc tgttccagca gttgcaggat
      301 tcaaacccaa atcagagttt agtatagcct tttcaatagc tgcgaccata gatttttccc
      361 agggtgtaac taaaatagtt cgggagtcac tggtagtgat gttggcaacc tggcttaaag
      421 gtgtaggact tccataataa tcaaccatga catgatctaa aagactggca ttcgccctgc
      481 cggtacggat ttttgacata tccgtatgca aagcctcaat agtctttttc atgcgttttt
      541 ctgaatcttg cttaatctca ttgatcatga ttagctccga ctatagttcc tactcgttct
      601 ccagttacaa ttcgttttaa tgctttagga gccgccatat caaacacctg taaaggcatt
      661 ccctgatctt ggcacaaaca aattgcagtg gaatccatca cttccaaacc tttggttaaa
      721 acttctttgt aagtcaaata atcataacgc tttgcatcag agtttttaag tgggtcagca
      781 gaataaactc catccacctt agttgctttt aaaacaatat ctgcgcctat ctcaattgcc
      841 ctcaaacaag cagcagtatc tgttgtaaaa aagggatttc ctgttccagc tgcaaaaatc
      901 accacctgac cattgcgcaa gtgagtgata gcttttcgtc tatggtatgg gtcaactacc
      961 ccaagcattg gaatagctga cataatacga gcgggcagat caattcgctc caaagcatca
     1021 cggagtgcca aagcattcat aacagtggcc agcatcccca tatggtctcc tgtcactcgt
     1081 cctacgcccg cctgagagag cgctttccct ctgaacagat tgcctccacc taaaaccaaa
     1141 ccaacttcca cacccatatg aattaattct gctatgtctc gagctaatga gtcaagaact
     1201 gaaggatcaa tcccaaattg agatttcccc atcagcgcct caccactaaa tttaagcaga
     1261 atgcgtttgt attttaattt aggttgactt tcattcatca tgtacgaacc tgggccatca
     1321 cttcttcaac aaaattgtct tcttttttct ctattccttc ccctacttca taacgaacaa
     1381 aggatattac ttcagcattt ttctctttta gaagttgacc cactttaatg ttaggatctt
     1441 ttacataagg ttgtcctaac aagcttacct catcaataaa cttattgatt cgtccatcaa
     1501 tcatcttatc aatgatctct tgcggcttac cactctcttt cgcttgagca gtaaatattt
     1561 ctctttcgtt ttcaatcgct tcggctggca cctgatcgcg actaaccacc atcggtttgc
     1621 ttgcagcaac atgcatggca atatctttag ccaatgcttc acttccattt tttaatgcaa
     1681 ccataactcc aatacgcgaa ccatgcaaat aatacccaat tacgccgtca caatgcattc
     1741 tttccaatcg gcgtaattta atattttcac caattttagc aactagttct tgtcttgctt
     1801 gctcaacagt agtaccagat gataatgcct gattggataa gtcttctata ttttttggaa
     1861 ggttggttaa tgcagcatcg gcaacagcat tggcaaaatt agtgaaattc tcatctcgtg
     1921 caacaaagtc tgtttcacta ttaatctcaa gcattaccgc tgtacgttca tcagaagaac
     1981 gtgcaataac gattatacct tctgctgcaa ctctatctgc ctttttgtct gcttttgcct
     2041 ggccagcttt acgcatttca atgattgctt gctcgatatc accgttagta gctattagaa
     2101 attttttaca ttccatcata cctgcgcctg tgcgctcacg caattgcatt actaatgcag
     2161 cactaattgt tgacattttg atttctccac atagcaaaaa gggggcttat taaattaacg
     2221 ccccctttat tcaaatagta attattctcc ggatttttct tcttcattag aatttgaagt
     2281 gacctcaaca aattctgaat cagatgatac tcttcctaca gtattgctat ttttaccatc
     2341 taaaatagca tcagcgacac agcgaacata aatgtccaca gcacgcatag aatcatcatt
     2401 gcctggtata acataatcaa tgttgtcagg gctattattg gtatcaacaa caccaattac
     2461 cggaattctt aatcgacgcg cttcttcaac agcaatatgt tcaaaaccaa catcaaccac
     2521 aaataacgca tcaggtaggc cgcccatgtt ctcgattcca ccaagactac gctccagctt
     2581 ttcaagctct cttgtcagca ttagcgcttc tttcttaatc atgtcattga acaatccctt
     2641 ttccttcatt tcttttaatt cttttaaacg gaatattgat tgacggactg ttttatagtt
     2701 ggtcagcatg cctcctaacc atctatggtc tacataaggc attccgcatc gttttgcatg
     2761 ctctcgaatg ctgtcttgag ctgctctttt agtgcctaca aaaagaattt ttgccttgtt
     2821 tgctgccaat cgactaacgt aattcgttac gtcattcagc atgggtaatg ttttttcaag
     2881 gttaatgata tggattttat ttcttgagcc aaaaatgtat tcactcattt ttggattcca
     2941 gaaacgtgtt ctgtgcccaa aatgagcacc tgcttcgagc aattcacgca tactaacatt
     3001 gttcatatat atctccaggg ttaagcctcc acgcttccca tacgaaaccc accaagggac
     3061 cctatcgcat gtgacgaaac gtgtgtgtat ttaaagcgcg gtatttataa catattcgtg
     3121 ctaattcagc aatgaaatta acatcgttct ctgtacatga caaaaatatg tcatgcataa
     3181 aggactatgt atgaccacag taattagaca aagcctatta ggaaataagt ctatattaat
     3241 ttgtacattt tgatttatac tgctatcagc atggaagttt taaaaatgtt gtactcagga
     3301 gaatcctatt aacgataaca tttaagagga aacgataatg agtaaaaaat ctatctgttt
     3361 agccacgctc gctttgatat ttacctttgg tcagcctgtc tttgccaact caagcgaagc
     3421 tacacaaaca aagccttgtc attgtcatga tcacaaaaaa atactcagtc agcttaattt
     3481 aactcaagag caaaaaacca aaatcaaaac aattaaaata caagctcaaa aatcaatcaa
     3541 agaaaattat agacaactta aagcgattaa agaacaaatc aatgcgctca ttgcaaatga
     3601 gaagcttgat gaagccaagc tggatagttt aatcaaccaa agaaataaaa tagcagggac
     3661 tatgctaaaa aatcgtgtaa tgatgaaaaa tcaaatttac aatcagctaa caaacgaaca
     3721 aaaatcacaa tacaaaaagc atatgtaaaa cttgattggt accgataaca aaaaattcgg
     3781 taccaatcta tcccaactct tcagatataa tcattgctgt aattatgatc tttctcactg
     3841 ttacttcctg aacaatattt tctgaacaca aaaaaatggg caaattttct ctctctacca
     3901 ttaaaaaaca gatgatattt cgctcagtat gctaacccat agtatgctgc agcagctgca
     3961 ctggcaatga tgcaaagtgc aaatattata gttctcgcca tattcttctt tgttttgggt
     4021 ttcataacaa atcctggaat gacggttatc ctgattgata tttcatttaa attatagttc
     4081 aaattttatg catagctaga cccacatatg gattgccctt acacagaata gcatggagct
     4141 ttaatacaaa ttcagttcaa aacacttgct ataattattg acttatggcc caatttagaa
     4201 caacctatat cggcgatgag caatattgtt gttgcagaat aaaaaagaaa cccaattatg
     4261 agaggacata tgattttttt aagtattcct aaagggatgg aatttaagca aattacagaa
     4321 aaagacaata cgaacgacta ttttgttgat ccgaatggaa agttaccacg aattgatatt
     4381 caggctttag ttaaagacgc tctgcaatat aacaaaggtc gaaaaaagga aattgctctg
     4441 tctgatttta ctatttatag acacaaaccg ccttataaag atgaactctt tcttcagtat
     4501 aatccagatc ataatggtaa atattttaca aaagaatctg taagtctggt gaatggtaaa
     4561 gaatttatta gaaataaagc tcctgcaacc tcatatggca ctttttggtt tcaaaaagta
     4621 caactatccg aaagcagaat ggatgaagtg cttgccaagc gaagtgaaca acgagaaaat
     4681 cgtaggcata cagggaatag tccaaaccca acataaataa aaaggtatgc acaggatgca
     4741 tacctttttt aattagatta aatagcaaat gcagcagagg ctttgttatc taattgatga
     4801 gtgaatttac tttgcatggt caaataggct cctactactt tataagactc tggtgaatag
     4861 aaatcttttg ttgagtattc tttagcataa gtgaggcctt ccagccgatt atttgctgcg
     4921 gttatcactt tgtctaaatc aatcaaggtg gtagagtaat tatcaccact gtctgttatc
     4981 attttcattt cttcaagagc ctcccgtaca acagtcagaa ttttattaaa atttggacca
     5041 gcagtagtgc aaccccgata gatagtaatt agacgatcga caagagagaa gaaattaacc
     5101 atcttttgca aatttgactc aagaatacgc atacatgcta ccatttctgt tgtgctgcgt
     5161 tgaggatatt tgtccatcaa agcattcatc acttcgacaa ctttttttac aaacccatca
     5221 acagctttaa cggaataact gtttttctta aatgcttcga aaatagattg ggcatttctc
     5281 attggatcct ttaaagcatt cacactttta ataaacacat taacctgctc ccaaaaacgg
     5341 ctgccatcct tcctgttttc tctagcttct tgatagtttg tgattaacat tttcgcagta
     5401 aaagcatatt gggataagaa aaagcgatca cttttctccg ccattatcgc ctgcaaagta
     5461 aaaattggat tgctccattg tccttcatca cacgggacat tccattgctg ggctttcgct
     5521 ctgattaaaa aggctttttg ttcatttgat aacgtagtga tagcgttgat ttttgcttta
     5581 tacaattcaa atgcatcaag ctttattttc tctacagctt gttcatgagc tttaattaat
     5641 tcagcgggag aattaaaatt attaacaaac ttcattggtt gtaacttatt ggcttcgaat
     5701 tcaaaattac aattgtattt tgcctgacga actcctgctg cattaatttc tttgccttcg
     5761 gtatcataaa tatctctggc atactcaatc gctttatcac cgaatgcttt gatcgtttta
     5821 gcatagtcag gatttgtaaa gccctttccc tgaacaggaa ttttgtaatt gtccaaggta
     5881 tggacatttt cacggttaca agcatatttt accaaatgca tcgtaacatt agatggaata
     5941 agctctggat attctgagaa aaattgatgc aacccttcaa gaaggtcttc tcggtcatca
     6001 taaaaaaaat aatcaatttt ctctctagct tccaaggaag aagctctttg catgtgtgca
     6061 tacacaagcg aagttttcga tttatcatgt aaccagccgt tttgaacttt tccgccattt
     6121 gcaggattcg ttgcctcatg gagactagta cctgctggca ccaaattgta tgcatcagct
     6181 aataaaaacg gatcaaatgc catgcctaaa tcggtgcaca gtttttgaat ctggataaaa
     6241 caagaaccat tgccattgga gcgagcatta cgcttatcat cgcgatcact ttgccgattc
     6301 gaagcaatta acgcaacttc tttagtatag tagtttgaag tttctcgaat tttttgggct
     6361 agttttggat tttgtgcaac gatatttatt agattggtaa caccgacgca cccgtttcct
     6421 catgaaacta gaaatagcca gtcctctagg taatccttat agagaagaca catcgaaatt
     6481 ttgctaaaca atattaagca aagttcataa tattaacatt atgtaaatat atgtgtcaat
     6541 tgcttattta taaaaataat aaatgttgtt tttctattca tagttcaaac agccccaatc
     6601 aggttcaatt tggactgtat aagctctcat ataacacgag ttaatagaga gctcagaatt
     6661 tctgatataa gatatgtaaa caggaaatat caaatcaaaa aaacttgctt tcaagtgtga
     6721 tacttgaatc cataatgcct aattgctcta acaagtcttc aggcaaatgc aaaccgctct
     6781 ccctgagc
//
LOCUS       DASLOE010000154         6770 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.155, whole genome shotgun sequence.
ACCESSION   DASLOE010000154 DASLOE010000000
VERSION     DASLOE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.155"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..800)
                     /locus_tag="U6309_002409"
     CDS             complement(<1..800)
                     /locus_tag="U6309_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEN5505840.1"
                     /translation="MDSLDLDFYAITRAFLFFSLIGFIVFLEYFFSFHRLGKAGFRRR
                     RISNWYLGIVNIIVALPLSTLTTAAYCEYYQIGLFNLLKVPFGLVFILWFIMYDLYVY
                     LFHRLSHATFLWHFHKIHHSDKSLNVTTSFRFHPVEYILFNALKIVLIILLGPYFFVI
                     LFSDFIQAILVFWGHSNIALNAKLEQALSRIIITPRYHVMHHTQDECRRNYAGGLTLW
                     DYLFHTQTEPLWNKDEINKLTLGVQTNTNWPEFKNLLLINKLIHFFDKP"
     gene            1124..1360
                     /locus_tag="U6309_002410"
     CDS             1124..1360
                     /locus_tag="U6309_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505841.1"
                     /translation="MKRVHHHPATHSHPSNATVQVSNGQHTVVVNPNPSYLPGQPPMY
                     HPNSFFTTHHHSPYYASQNQHYHQQPAPNTHQHP"
     gene            1538..2398
                     /locus_tag="U6309_002411"
     CDS             1538..2398
                     /locus_tag="U6309_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505842.1"
                     /translation="MISYLFSEVHFTHALKKVLGISVLVGVYSAIIYFWFEQDNLHSS
                     NWLSYSQSVFGIILGLLLVFRSNRAYERWWEARTLWGQLVNASRNLAIKIRILLSPNA
                     DEAKKFSELINTFCEVLAIHLRQKSNRDDFKKFLTRASLPNHVPSYVAQELYRELSEK
                     TKASKRLNLWLLDNEFSKFMDICGGCERIKNTFISLSFRVFVKHVFIIFILFLPCSLI
                     DTLGVWAIPATIIITYLIIAIEGIARNLEEPFGLTEDHLNLGSITESIRESVNEVLLG
                     QNSNEESNSI"
     gene            complement(2713..3414)
                     /locus_tag="U6309_002412"
     CDS             complement(2713..3414)
                     /locus_tag="U6309_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEN5505843.1"
                     /translation="MNRYSIFFLVFCAFSLESHAGLYSGINLGINAVKINKNLVYPLE
                     DPVPTTSSFNSAYTDFHGQLIAGYDFSLTSKISAAVEANIDLFTGKARHRITEWYFDE
                     DVYAEEQLKVGYSLFLLPTYHLNDMVQLFVGPGVSSSRFNIAFSNTAGNVGVSDSLNE
                     WLTGISIKAGSITKLTRNIDLLLTYQFTQYNSVTGVQMEPLSEDTLRGSYKPNVNTVL
                     IGFKVHPSDEITKDT"
     gene            complement(3427..5643)
                     /locus_tag="U6309_002413"
     CDS             complement(3427..5643)
                     /locus_tag="U6309_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505844.1"
                     /translation="MIRTFLTAFLMLLCSLALAQEKVVTHFFKEVNSSTAISEGKANI
                     QVSRYRVVELDINQLYAELENVPHRDGISTGTPLQFELPQPDGTVKRYQVMENSTLAP
                     ELSTKFPEIKTYDAYGIDNPGELVKFDLTPQGFHAMILSPGRNTVFIDPLIKGNTQYY
                     MIYYKKDFITSKKMKCGVKSQNQPLINTASKRDFTDFNPCVLKKYRLALAATAQYTQF
                     HGGTVPQALAAETTTVNRVNGIYEIDMAITMQIIANNNLIIYTDPNNQPYTSGDPDKM
                     INENQANIDQVIGAANYDIGHVVDAAGSGLAQTPSVCINGEKAMGVTGQSNPVGDPFD
                     VGYVAHEIGHQFGANHVQNNNCQRNNPTAVEPGSGSTIMSYAGICDPNVQDDSDPYFN
                     GISLQEMGNFVSDAAHTCPVKTSIPSAPVIQGTNGGVKIPAQTPFALTATATKSGGNE
                     VLTFAWEQQNNEASQQPPVSTSRVGPNFRSFSPQVSGTRFFPNLNALANNGPFTWEVL
                     SSVSRTLKFRVTVRRNTPGGSCNAYTDTTLSVESKAGPFVVTNPTESGITWTGISPRT
                     VTWDVANTNIPPINARFVNILLSIDGGQTFPFTLLSNVDNTGSEVICVPNLNSSTARI
                     MVQASNGTFFNVSKNNLTIIPVPPRPPELTRADRNPMNTSEAFVLYANCIPTGNDVYT
                     VNGLPPGATVRFDANNRRFVIENINTPKRVRNVTITATDENGVSRTSNAITIPSIL"
     gene            5848..>6770
                     /locus_tag="U6309_002414"
     CDS             5848..>6770
                     /locus_tag="U6309_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEN5505845.1"
                     /translation="MDPITHGVLGAACAQAALGKYDKRIPWQAGALAGMAPDLDVFIR
                     FSQEPLSLEQWHRYLTHSLMFIPIGGLIVALFLLCFSQYRKYWKVTIGASIVGYATHC
                     LLDSLTSYGTVLFWPWSDSRISWDIISIIDPVFTLPLLLGTAWSVIYQNQKAVTASLF
                     FCILFLIFKTILHNRAVDGIHQYATTNNFKINHPRALPKLASSTYWRIIAKQQDCLII
                     AEAKTSLFYPTTIRLIRQALSFNESMIPFALSSGQKSDLATFHWFTDGFLIVANKNPL
                     ILADARYTIDDNPIFSLWGIEVLPGQEHINK"
BASE COUNT         2129 a         1288 c         1227 g         2126 t
ORIGIN      
        1 ggtttgtcaa aaaaatggat taatttattt attaataaga gatttttgaa ttcaggccaa
       61 tttgtatttg tttggactcc aagggttagt ttattgatct catctttatt ccaaaggggt
      121 tctgtttgag tatgaaataa atagtcccat agtgttagtc ccccagcata atttcttcta
      181 cattcatctt gcgtatgatg catcacatga tatctcggag taataattat tcgagatagt
      241 gcttgctcaa gtttagcatt cagggcaatg ttgctgtgtc cccagaatac aagaatggct
      301 tgaataaaat cggagaataa tatgacaaag aaataaggac ccaaaaggat aatcaaaact
      361 atttttaagg cattgaataa aatatattca acaggatgaa atctaaaact cgtcgttacg
      421 ttaagcgatt tgtcggaatg atgtattttg tgaaaatgcc ataagaaagt cgcatgactt
      481 aggcgatgaa ataagtacac atacaaatca tacattataa accataaaat aaagacaagc
      541 ccaaaaggta ccttcagcaa gttaaataat cctatttggt aatactcaca ataggccgct
      601 gtagttaagg tgcttaatgg aagggcaact ataatgttaa cgatacccaa ataccaattg
      661 ctgattcttc gccttctaaa ccctgcttta cccaaacgat gaaaactaaa gaaatattca
      721 agaaatacta taaaaccaat tagcgaaaaa aataaaaaag ctctggtaat agcataaaaa
      781 tctaaatcta gagaatccat aacaaaacct taacaaatct ggttaaacac aaagaaaaag
      841 aagccccccc cttggtgctt attccttagc ttattaagtt atgttaatta tcttgactag
      901 aatagtgttt ttgtgtgccc actatgctga tgattatagc acaaaaagcc gataatattg
      961 aggtgaagaa agccaagaaa gcgaaaatag taaattttta atactatctg tacttgttta
     1021 actatctaca ttacaatctc ggacacaggc tattctgaaa taacctgagt aaagtcagtt
     1081 ttatgtatgt catgcagttg tatttttata aggataataa tctatgaaac gagtacatca
     1141 tcaccctgca acacactctc acccatcaaa tgctactgtt caagtcagca atggacaaca
     1201 cactgtagtt gttaatccaa acccgtctta tcttccaggc caaccgccca tgtaccaccc
     1261 caatagtttt tttacaacgc atcatcactc accctattac gcttctcaga atcaacatta
     1321 tcaccagcaa ccagcaccca atactcatca gcacccataa atggtggtaa ctgttaacaa
     1381 acaggctatt actctttaag aatttagact gaaaaacaag taataactgg cttctctggt
     1441 tattacttgt tttgctctca cgtttttttg acaaagccct ttatagaaag atcggtttct
     1501 attattctgg aaaaacaaaa caatcaaatg gactgccatg attagctatt tattttcaga
     1561 agtacatttc actcatgctc tgaagaaagt acttggaatt agtgttttgg ttggtgttta
     1621 cagcgccatt atttattttt ggtttgagca agataacttg cattccagta actggctatc
     1681 ctattcgcaa agcgtatttg gcatcatttt aggtttgctt ttggttttta gaagcaatcg
     1741 ggcttatgaa cgttggtggg aagcgagaac cctgtggggt caattagtca atgccagtcg
     1801 taatttagca ataaaaatca ggatattgtt atcacccaat gccgatgagg ctaaaaaatt
     1861 ctctgaactc atcaatacgt tttgtgaagt attggccatc catttgagac aaaaaagtaa
     1921 ccgggatgat tttaaaaaat ttttaacaag ggcgagtctt cccaatcatg tgccctctta
     1981 tgttgcacaa gaactgtatc gtgaactttc tgagaagact aaagcatcga aaagattgaa
     2041 tctatggtta ttggataacg aatttagcaa atttatggat atttgcggcg gttgtgaacg
     2101 tattaaaaat acatttatct cgctttcatt tcgggttttt gtaaagcatg tattcattat
     2161 ttttattctt tttttacctt gcagcctaat cgatacgctt ggagtttggg caatacctgc
     2221 taccattatt atcacctatt taatcatcgc catagaggga attgctcgca atttagaaga
     2281 accatttggt ttaacagaag atcacttaaa ccttggttca ataacagaaa gtattagaga
     2341 aagtgtcaat gaagtcttgc taggacagaa ctctaatgaa gaatccaatt caatttagta
     2401 tggattataa ttcataaaag tttttctact taaataaaag ggagtcatta taatggaatg
     2461 taaatgcaat caatgtggta tgggcgtaaa aaatttaacc tgcagtaaat gtgacgcatt
     2521 gttggttagt ggcaccatag tcaaaaatga tgggaataaa gttcaagtag caaaatgccc
     2581 caacggatgc ggtcaaatca aatcacctgt ttgctgcgga cacgatatgg cgtgctcagt
     2641 ctgattattt aaaatcagtt aaggatagga aacctcaaat aactgcaatt gaggtcttat
     2701 ccataactga gattaagtgt cttttgtgat ttcatctgaa ggatgtactt taaagcctat
     2761 tagcactgtg tttacgtttg gcttatagct ccctctcaac gtatcttcgg ataaaggttc
     2821 catttgaact ccggtaacac tgttatattg tgtgaattga taggttaaaa gtaagtctat
     2881 attgcgagtc agcttcgtta ttgaacccgc cttaatgctt atcccggtta gccattcatt
     2941 caaactatca ctgactccta cgtttccagc agtattacta aaagcaatat taaatcgact
     3001 tgatgacacg cctggaccta caaacaattg aaccatgtca tttaaatgat aggtgggtaa
     3061 caaaaataac gaataaccta cttttaactg ctcttctgca tacacatcct catcaaaata
     3121 ccattcggta attctgtgtc ttgccttacc tgtaaacaag tctatatttg cttcaactgc
     3181 agcagatatt tttgaggtaa gagaaaaatc ataacctgcg atcaattgtc catgaaaatc
     3241 agtataagca ctattgaaac tggaagttgt tggcacaggg tcttccagcg ggtagacaag
     3301 atttttattt atcttaacag cattgattcc taaattaatt ccagaataca aaccggcatg
     3361 agactccagt gaaaaagcac aaaaaaccaa aaaaaatatg gaatatctat tcatattgag
     3421 ccctttttat aaaatactgg ggatagtaat tgcatttgaa gttctgctaa caccgttttc
     3481 atccgttgcg gtaatagtca cattccgcac tcttttcggt gtattaatat tctcaatgac
     3541 aaatcgtcga ttatttgcat caaatcttac tgttgcacca ggaggcaatc cattcaccgt
     3601 atacacatca ttgccagtcg gaatgcaatt cgcatagaga acaaaagcct cagaagtatt
     3661 catagggttt cgatcagcgc gcgttaactc tggtggtcga ggtggaactg gaataattgt
     3721 caaattgttt ttggaaacat taaaaaaagt tccatttgat gcctgcacca taattcttgc
     3781 tgttgacgag ttcaggttag gaacacagat tacctcactt cctgtattgt cgacatttga
     3841 taagagggta aaaggaaaag tttgccctcc atctattgat aatagaatgt ttacaaatcg
     3901 ggcatttatt ggaggaatgt tagtattggc tacgtcccaa gtgactgtcc ttggtgagat
     3961 accagtccag gtaatacctg actcggtggg attcgtcact acaaaaggtc cagccttact
     4021 ttctacagat aaggtagtat cagtataagc attgcaagaa cctcccggag tattcctgcg
     4081 tacggttacc ctgaacttca aggtacgaga aactgaagat aaaacttccc aggtaaaggg
     4141 accattatta gccaaggcat ttaaattggg gaaaaaacgt gtccccgata cttgaggaga
     4201 gaaacttcta aaatttggtc caactcgtga ggtactaaca ggaggctgct gtgatgcttc
     4261 attattctgt tgctcccagg caaaagttaa tacctcatta cctccacttt tagttgctgt
     4321 agcagttaga gcaaaaggcg tttgtgctgg tattttaacc ccaccattcg ttccttgaat
     4381 taccggagca gagggtattg atgtttttac aggacaagta tgcgcagcat cagaaacaaa
     4441 attccccatt tcttgcaaac taataccatt aaaataggga tcagaatcat cttggacatt
     4501 agggtcgcaa attcccgcat aactcataat cgtactgccg cttccaggct ctacagcagt
     4561 aggattattt ctttgacaat tattattttg tacatgattc gcaccaaatt gatgtcctat
     4621 ttcatgggca acataaccga catcaaatgg atctccaaca ggattacttt gcccagttac
     4681 acccattgct ttttcaccgt ttatacacac gcttggagtt tgagccaatc cactgccagc
     4741 ggcatcaacc acatgaccta tatcgtaatt agctgctcca atcacttgat caatatttgc
     4801 ctgattttca ttaatcattt tatcaggatc gccgctggta tagggctggt tattcgggtc
     4861 tgtataaata atcaaattgt tattagcaat aatttgcatg gtgattgcca tatcgatttc
     4921 atagataccg ttgaccctgt tgacagtagt cgtttccgca gcaagtgctt gaggtactgt
     4981 acccccatga aattgggtat attgtgctgt tgccgccaat gcaagacgat atttttttaa
     5041 tacacaagga ttaaaatcag tgaaatctct ttttgaagcc gtatttataa gaggttgatt
     5101 ttgacttttc acaccgcact tcattttttt ggaagtaatg aaatcttttt tgtaataaat
     5161 catgtagtac tgcgtattac cctttattaa aggatcaata aagactgtat ttctgcccgg
     5221 acttaagatc atggcatgaa agccttgagg tgtcagatca aattttacta attcccctgg
     5281 attatcaatc ccataggcgt catatgtttt aatttctgga aattttgtgc ttaattcggg
     5341 agctaaagtg ctgttttcca tcacttgata acgttttaca gttccatcag gctgaggaag
     5401 ttcaaattga aggggtgtgc ctgtgcttat accatcacga tgaggcacat tttccaattc
     5461 tgcataaagt tggttaatat cgagctcaac aacacgatat cggcttactt ggatatttgc
     5521 ctttccttct gagattgcgg tggaagaatt aacttcctta aaaaaatgag taacaacctt
     5581 ctcttgtgct aaagcaagtg aacaaagcag catgaggaat gctgtaagaa acgtccttat
     5641 cattgtaaaa cccatcaaat tgagtaaaat caattaccat tagaacagta aattcaatta
     5701 aaattgaaaa aattactttt attttttaaa aagtatctat aaaaactttt tagcaacata
     5761 taatacttat tttttcaatg aaattaaagc cttacctttc tcaaaagaag cgcattttca
     5821 ttgtattatc agcgtgttaa gtaaaaaatg gatcctataa ctcacggtgt tttaggtgcc
     5881 gcttgtgcac aagcggcttt aggaaaatat gacaagagaa tcccctggca agcaggtgcc
     5941 ttagcaggga tggccccgga tttggatgtt tttattcgtt ttagccaaga gccattgagc
     6001 cttgagcaat ggcaccgtta ccttacgcat tctttaatgt ttatcccaat tggaggcctg
     6061 atagttgcac tgtttctatt gtgtttttca caatatagaa aatattggaa agtaaccatt
     6121 ggagcctcta tcgttggtta tgcaactcat tgtttattgg attccttaac ttcttacggc
     6181 accgttttat tttggccctg gagcgatagt cgtattagtt gggatattat ttctattatt
     6241 gaccccgtat ttaccttacc tctattgtta ggaacagcct ggtcagtcat ctatcaaaat
     6301 caaaaggcag taactgcaag cctgtttttc tgcattttat ttctgatttt taaaacgata
     6361 ttacataatc gcgccgtaga tggcattcac caatacgcta cgactaataa cttcaaaata
     6421 aaccatcctc gtgccctgcc taaattggcc agctctactt attggcgcat catagctaag
     6481 caacaagatt gtttaattat cgcggaagcg aaaacttctc ttttctatcc aaccacaatc
     6541 agactgattc ggcaggcttt aagctttaat gaatcaatga ttccttttgc tttgtcatcc
     6601 ggacaaaaaa gtgatttggc tacatttcac tggtttaccg atggatttct aattgttgcc
     6661 aataaaaacc ccttaatcct cgcagatgcg agatatacga tagatgacaa ccccatattc
     6721 tcactatggg gaatagaggt attgccaggg caagaacata ttaataaatt
//
LOCUS       DASLOE010000155         6769 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.156, whole genome shotgun sequence.
ACCESSION   DASLOE010000155 DASLOE010000000
VERSION     DASLOE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.156"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            136..816
                     /locus_tag="U6309_002415"
     CDS             136..816
                     /locus_tag="U6309_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN5505846.1"
                     /translation="MNIKEKIGQRIQEERIAKGLTRKALEELTDDLKQSRISNWERGD
                     RTPGPEEIKQLARALDISPAYLMCLTDEKRPKKIPGLGSLVPLLDHQQACDPKGFIQR
                     VRNEHNSDEISFIPISSELSTRLGENAFALKMKDESMHPELRVNDILIIDPNSELHPG
                     SLVAAQLNEENEVIVRRYKQLTLSKEFNPFELRAENDNWGNIKIDKVTDAIIIGVIVC
                     VVRYTGRY"
     gene            965..1639
                     /locus_tag="U6309_002416"
     CDS             965..1639
                     /locus_tag="U6309_002416"
                     /inference="COORDINATES: protein motif:HMM:NF024935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN5505847.1"
                     /translation="MDMLDLYCDYLIFQNKYATATGLSDLVDGAFAHNKVTRFLRFEH
                     FGSKSLWHYVKKPVRSQETVGGVLILDDSIEEKPYSDENEVNCWYYSHAKGAVLKGIN
                     ILYCMVRYADFSVSIGYKVIKKDIAYCDIKTRQTRRKSSVNKNQLFQSLIAQAITNKV
                     MFDYVLADNWFGSKANMVHIHKDLQKSFIIGIKSNLTLALSENDAKNGRYQQVRALEL
                     EEDILA"
     gene            2088..2660
                     /gene="msrA"
                     /locus_tag="U6309_002417"
     CDS             2088..2660
                     /gene="msrA"
                     /locus_tag="U6309_002417"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN5505848.1"
                     /translation="MRYLFILIALLSQPLFAKPSEAIFAGGCFWCVEADFDKVPGVLE
                     TISGYDGGTEPRPGYALVSSGKTAYVEVVRVVFDSDKVTYQELLDFYWRHIDPTVQNQ
                     QFCDVGKQYRSVIFYLNDDQKKAALASIDEIKKRFPKVYTEVIPSTTFYPAEEYHQDY
                     YQKNPIRYKYYRYNCGRDARIKELWDEKSS"
     gene            2644..3141
                     /gene="msrB"
                     /locus_tag="U6309_002418"
     CDS             2644..3141
                     /gene="msrB"
                     /locus_tag="U6309_002418"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN5505849.1"
                     /translation="MKSLVSFIVSVFMLNTALAYTPEFNKSEKLKQLTPLQYQVTQKA
                     ATEKPFDNPYWNNEQAGIYVDIVSGEPLFSSLDKYDSGTGWPSFTKPINTANVILKPA
                     RHYLFFVQTEVLSKNAQSHLGHVFNDGPKPTGERYCMNSAALRFIPKDQMSKEGYEEY
                     LTLFK"
     gene            complement(3134..3721)
                     /locus_tag="U6309_002419"
     CDS             complement(3134..3721)
                     /locus_tag="U6309_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN5505850.1"
                     /translation="MIEQHLRPLYQRLCVNPITPVLIERVTPNQVTFFSGVLGLLVIP
                     ALWLNLPYLAISLLLLSGYCDTLDGTIARLTNHSSDWGSVLDIMTDRVVEVGVVFALW
                     AINPNERGLGSLLMMASILLCITSFLVVGIFKTNDSEKSFHYSPGLIERAEAFVFFIA
                     MMLWPNHFLSLAMIFSLLVIGTAMHRLYEFHKQSI"
     gene            complement(3726..5861)
                     /locus_tag="U6309_002420"
     CDS             complement(3726..5861)
                     /locus_tag="U6309_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN5505851.1"
                     /translation="MKISAIKTWLPLLIIIVLLGLFLGLGLNKYVSFASLRDNHEWLI
                     AITKSHFYLVSFVFIIIYTVAVALSIPGAIFLTLIGGFLFGILWGTFLVVLSATLGAT
                     ILFFAVQSSLGEVFSKRASGWVKRMRSGFKDNAFSYLLTLRLIPLFPFWVINIVSAVL
                     GVSASTFIIATFIGIIPGSIVYVSVGNGLGELFAASLQPNLGIIFEPKFILPLLGLAA
                     LSLIPVFYKKKGKQGTEKMKDKDLFCDLAIIGGGSAGLSIAAGCSQLGLQVVLVEPNK
                     MGGDCLNYGCIPSKTLLNTAKIFYQTKHSTVLGIQAKSVKIDFLQVMQQVHNVIACIA
                     KNDSVERFTSLGVQVIQEAGHFIGPKQFKLKRKIIRAKHFVIATGSSPAIPPIQNLNK
                     VSYLTNETIFNLKVQPEHLIVIGGGPIGCELAQAFAMLGSKVTILEAFTILPKDDADC
                     VAIIRSQLESMQIALYEQIKINKIEESADKTISVHLENQDNPITITGSHLLVSTGRVA
                     NVDGLGLEKAGVQYTTRGINTNARLQTTNKKIYAIGDVTGPYQFTHMASYQAGIALRN
                     IAFKWPAKVDYKAVPWVTYTEPELAHVGLLASEALKDPTLKITEFAFAEVDRAQAENK
                     VNGKIKIITGKKAKILGVSIVGAHAGELILPWVIAVKERKSLRTFTDAIAAYPTFSEI
                     SKRVAGEYYKPFIFSSKIRTFVRWLNKLG"
     gene            complement(5880..6761)
                     /locus_tag="U6309_002421"
     CDS             complement(5880..6761)
                     /locus_tag="U6309_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEN5505852.1"
                     /translation="MSEINYSTVQYYLVLILIFIYLSLFVLEKKWPLRKATRPLLPRL
                     AINFTFTLLVYVIASLFISPLAKATVLFTFSHDFGLLSMLPFNQWVVFILGFLLMDLS
                     FYYWHALNHKLPLLWRFHVVHHADPDLDVSTAMRFHCVEIMYSSLFRLVQLGLIGVSP
                     LLFFCYEFFFQAHTFFQHSNIKLPFRFERLINKIIVTPRMHGIHHSNYLNETNSNYGV
                     VFSFWDRLHQTIKLDIPQQAIIIGVPGYSKPSDNSLIRLLLMPIKAQRHYWQIKKDFH
                     FSRKSKTSDKKVRTLCE"
BASE COUNT         2267 a         1274 c         1264 g         1964 t
ORIGIN      
        1 ttttttatat ggtatattta ctattaacta ctacttgagt cgtacgattc gtgtaaataa
       61 ttattttaat ttaccactat ttggtgcatt gtcaatatat attttcacaa tatgggatta
      121 attgggttgg ttaatatgaa tataaaagaa aaaattggtc aaagaattca agaagaacgg
      181 atagcaaaag gattaactag aaaggccctt gaggagttaa ctgatgatct taaacaatca
      241 agaattagca attgggagcg aggtgataga actccagggc ctgaagaaat aaaacaactt
      301 gctagagcac tagatatttc acctgcttat ttgatgtgcc taaccgatga gaaacgtcca
      361 aaaaaaattc caggattagg gtcattggtt ccactgctcg atcatcaaca agcctgcgat
      421 cctaaaggat tcattcaaag ggttaggaac gaacataatt cagatgaaat aagctttata
      481 ccgatttctt ctgagcttag tactcgtctt ggagaaaatg catttgctct aaaaatgaaa
      541 gatgagagta tgcaccctga attaagagtt aatgatattt tgattattga tcccaattct
      601 gaactgcatc caggaagttt agttgcagca cagcttaacg aagaaaatga ggttatcgtg
      661 cgtagatata aacaactaac cttatcaaaa gaatttaatc catttgaatt gcgggccgaa
      721 aatgataatt gggggaatat aaaaattgat aaagtaactg acgctataat tatcggcgtt
      781 attgtgtgtg ttgtgaggta tacagggaga tactaataca tttaacctaa ttaattcgat
      841 ttaccagtga aatgttaacg gatagttctg agatttttta taaaccactc atgttacgca
      901 cgtgaacttg cagtttgaat aaaaagcatt agaattttgc ccttaatttt agggagtgaa
      961 ttcaatggat atgcttgatc tttattgtga ttatttaatt ttccaaaaca aatatgcgac
     1021 tgctaccggg ttatctgatt tggttgatgg agcatttgct cataacaaag taacacgatt
     1081 tcttcgtttt gaacattttg gttcaaaatc actttggcac tatgtcaaaa agccagttag
     1141 gtcacaagaa acagtaggag gagtattgat acttgatgat tccatcgaag agaagcccta
     1201 ctctgatgag aatgaagtga attgctggta ttactctcat gctaaagggg ctgtactcaa
     1261 agggataaac atcttgtatt gcatggttcg ttacgctgac ttcagtgtat ccataggata
     1321 taaggtgatt aaaaaagata tcgcttattg tgatattaaa accagacaaa ccagaagaaa
     1381 atcatcagtg aacaagaatc aattatttca aagcttgatt gcacaggcta ttaccaataa
     1441 ggtgatgttt gattatgtgc tagctgacaa ctggtttggc tcgaaggcca atatggttca
     1501 catccataaa gaccttcaaa agtcgtttat cattggaatc aagtctaacc taacattagc
     1561 cttatctgaa aacgatgcca aaaacgggcg gtaccaacaa gtaagagcat tagaacttga
     1621 agaggatata ctggcttaaa ggcctagacc tccccgtgag gctcctgaaa aaggttttca
     1681 aaaacgaaaa tggctcaaca ggaattctct atcttgtttc aaatgacatg caaagtagtg
     1741 ccgaacgtct ttatgaagtg taccctggcg gattgaagag tatcacaagt caattaagca
     1801 aaatgcaagc ttaaccaagt ccccagctcg aatcgtcata acacagtgca atcatattta
     1861 tgcgtctatt attgcttact gcaagctcga aatgctgaag ataaaaaccc aattaaatca
     1921 ctttgccatt aagtacaaac taattgttag agcgaaccaa attgcctggc aggggcttaa
     1981 aaatatggct gcttaaaatt gatcgtgggt aacatgagta tttgagctaa aaccttaaag
     2041 taattttctg ctacacttaa aatacaaact gtcttccaag gttaggtatg cgctacctct
     2101 ttattttgat agctcttcta tcccaaccac tttttgctaa accatccgaa gccatctttg
     2161 ctggtggttg tttttggtgt gttgaagccg attttgataa agttcctggg gttttagaga
     2221 ctatttcagg ctatgatggt ggtactgagc ctaggcctgg ttatgcattg gtctcatcgg
     2281 gtaaaactgc ttatgtagag gttgtgcgtg ttgtctttga tagcgataaa gtgacctatc
     2341 aagaattact agatttttat tggcgtcata tcgacccaac agtacaaaac cagcaatttt
     2401 gcgatgttgg taagcaatat cgcagcgtca ttttctattt aaatgacgat caaaaaaagg
     2461 cagctttagc tagtattgat gaaataaaaa aacgatttcc taaggtatat acagaggtaa
     2521 ttccctctac aacgttttat cctgcagaag aatatcatca agattattat caaaaaaatc
     2581 caattcgtta caaatattac cgttacaatt gtggtcgtga tgctcgtatc aaggagttat
     2641 gggatgaaaa gtctagttag ttttattgta agcgtattta tgctaaatac tgccctggct
     2701 tacacgcctg aatttaacaa atccgaaaaa ctaaaacagc ttacgcctct acaataccaa
     2761 gtgactcaaa aggctgctac agaaaaacct tttgataatc cctattggaa taatgaacaa
     2821 gccggaattt atgtggacat agtttcagga gagcctctgt ttagttctct ggataaatac
     2881 gactcaggaa ccggttggcc tagttttaca aaacccataa ataccgcaaa tgttatttta
     2941 aaacccgcga gacactattt gttttttgtg caaactgaag tgctttctaa aaatgcgcaa
     3001 tctcatttag ggcatgtatt taatgacggc ccaaaaccca ccggtgaacg atattgcatg
     3061 aattcggcgg cattgcgatt tatcccaaaa gatcaaatga gcaaagaagg ctatgaagag
     3121 tatttaactc tttttaaata gattgcttat gaaattcata aagcctgtgc atcgctgtcc
     3181 ctatcactaa caaagaaaag atcattgcta aacttaggaa gtggtttggc caaagcatca
     3241 tggctataaa aaaaacaaag gcttcagctc tttctattaa tcctgggctg taatgaaaac
     3301 ttttttctga atcattagtc ttaaaaatac ccacaacaag aaaactcgtg atacaaagta
     3361 gtatactcgc catcattaac aaagacccaa gaccccgctc atttggatta atagcccata
     3421 aggcaaacac aacacccacc tcaaccacgc gatcagtcat aatatccaat acactacccc
     3481 agtcagagct atggttagtt agacgagcaa tagtaccatc gagggtatca caatatccag
     3541 acagtaatag taaactaatg gccagatagg gtagatttag ccacagtgcg ggtatcacta
     3601 aaagacccaa tacgccagaa aagaaggtga cttgatttgg ggtcactcgc tctatcaaca
     3661 caggagtaat tggattgaca caaaggcgtt gatacagtgg cctaaggtgt tgttctatca
     3721 tagatttatc ctaatttatt tagccaacga acgaaagttc gaattttaga agaaaatata
     3781 aacggcttat aatactctcc cgcaactcgc ttactaattt cactaaaagt ggggtacgct
     3841 gcaatcgcat cggtaaaagt tcgcaaactt ttccgttctt taacggctat aacccaaggt
     3901 aaaattaatt cacccgcatg agcgcccact atgctcacac ccaaaatttt agctttttta
     3961 ccggttatga tttttatttt gccatttacc ttattttccg cttgggctct gtccacctcg
     4021 gcgaaggcaa attctgtgat ttttaatgta ggatctttta aggcctcact cgccaatagg
     4081 cccacgtggg ctaattcggg ctcagtataa gtcacccaag gcaccgcttt gtaatctact
     4141 tttgcaggcc atttaaaagc aatatttctc aatgcaatac ccgcttgata actagccatg
     4201 tgcgtaaatt gataaggccc tgtcacatcg cctatggcat agattttttt attagtggtt
     4261 tgaagtctgg cattcgtgtt tatccctctg gtggtgtatt gaacgcctgc tttttccaaa
     4321 ccgagcccgt ccacattagc cacacgacct gttgatacca acaaatgcga gcctgtgata
     4381 gtaattgggt tgtcctggtt ttccaaatga acactaatgg tcttatcagc actctcttct
     4441 attttgttaa ttttaatttg ctcataaagt gctatctgca tcgactcaag ttgacttcga
     4501 ataatagcca cgcaatcggc atcatcctta ggaagaatag taaaggcttc taaaatagtg
     4561 actttagacc caagcattgc gaaagcctga gctaattcac agcctatagg acctccgccg
     4621 ataacaatca aatgctcagg ttgtactttt aggttaaaga tggtttcatt agttaaataa
     4681 gagaccttgt ttaagttctg gataggaggg atggctggag atgagcctgt agcaatcaca
     4741 aaatgtttgg cccgaataat tttccttttt aatttaaact gttttggccc tataaaatgc
     4801 cctgcttctt gaataacctg aacacctaga gaggtaaaac gttccaccga atcatttttt
     4861 gcaatacacg ctatcacatt atgcacctgc tgcatcactt gcaaaaaatc aattttcacc
     4921 gattttgcct gaatacctaa aacagtcgaa tgtttggttt gataaaagat tttggcagtg
     4981 ttcaatagcg ttttagaagg gatacaaccg taatttaaac aatcacctcc cattttgttt
     5041 ggttcaacca ataccacttg taatcccaat tgagagcaac ctgcagcgat acttaagcct
     5101 gcagaacccc cgccaataat ggccaaatca caaaataaat ctttatcttt cattttttca
     5161 gtaccctgtt tacccttttt tttgtaaaaa acaggaatca aagaaagagc ggctaatcct
     5221 aataaaggta gaataaattt gggttcaaaa ataatcccca gattaggctg aaggcttgct
     5281 gcaaatagtt cgcccaatcc attaccaaca gacacataga caatagagcc aggaataatt
     5341 cctataaaag tggctatgat aaaagtagaa gcgctcacac ccaatacggc agaaacgata
     5401 ttaatcaccc agaaaggaaa taatgggata agtcgcaagg tgagcaaata agaaaatgca
     5461 ttgtctttaa acccagaacg catccgctta acccaacctg aagcacgctt ggaaaacact
     5521 tcgcctaagg acgattggac tgcaaaaaaa agaatcgttg cacctaaagt ggcacttaga
     5581 accaccaaaa aagttcccca aagtattcca aataagaagc cgcctattaa agtcaaaaag
     5641 atagcacctg ggatagagag tgctacagcc actgtataaa ttattataaa aacgaaagag
     5701 accagataaa aatgtgattt tgttatggct ataagccatt catgattgtc tcgtaaactg
     5761 gcaaaactta cgtatttatt taagcctaaa cccaaaaaaa gacctaataa aactattata
     5821 attaataagg gcagccaagt ttttattgca ctaattttca taagatgatg cccttttcat
     5881 tattcacata acgttcttac tttcttatca gaagtttttg actttcgact aaaatgaaaa
     5941 tcctttttta tttgccaata gtggcgttga gctttaattg gcatcaaaag taaccgtata
     6001 agactattat ctgatggctt ggaataccca ggtacaccaa ttattatagc ttgttgggga
     6061 atatctaatt tgattgtttg atgcaaccta tcccaaaatg aaaatacaac cccataatta
     6121 ctattggttt cattgagata attggaatga tgaatgccat gcattcttgg agtaacgata
     6181 attttattaa ttagccgttc aaagcgaaaa ggcaatttga tattgctgtg ttgaaaaaaa
     6241 gtatgtgctt gaaaaaaaaa ttcatagcaa aaaaagagta agggactcac tccaatcaac
     6301 cctaattgca ccaacctaaa gagactcgaa tacataattt ccacacaatg aaaacgcatg
     6361 gcggttgaaa catctaagtc aggatcagcg tgatgcacga catggaatcg ccacaacaac
     6421 ggaagtttat gatttagagc atgccagtag taaaagctta aatccattaa caaaaaccca
     6481 agtataaaca ctacccattg attaaaggga agcatagata gaaggccgaa atcatggcta
     6541 aaggtgaaaa ggacggtggc ttttgctaag gggctaataa acagacttgc gatgacataa
     6601 accaagaggg taaaggtgaa attaattgct aatcgcggca ataatgggcg agttgctttt
     6661 cttaaaggcc actttttctc taaaacaaac aatgacaaat aaataaaaat aagaatgagt
     6721 actaagtaat attgtactgt actgtaattg atctcagaca ttagtttaa
//
LOCUS       DASLOE010000156         6654 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.157, whole genome shotgun sequence.
ACCESSION   DASLOE010000156 DASLOE010000000
VERSION     DASLOE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.157"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(400..1368)
                     /gene="lspK"
                     /locus_tag="U6309_002422"
                     /gene_synonym="gspK"
     CDS             complement(400..1368)
                     /gene="lspK"
                     /locus_tag="U6309_002422"
                     /gene_synonym="gspK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspK family T2SS minor pseudopilin variant LspK"
                     /protein_id="HEN5505853.1"
                     /translation="MFMSSPPPKSIASSKMQGSALLTALFIMTLVAIVATAMSTRLQQ
                     DIYRTRLVITQDKLYLASQAVTFWALNELLDKNNRFTKTNQLGMVAQYPKNMESIYNQ
                     VQLSGGIFDLQARFNLNNLIEKKSIPTLMHLISHAYSKATGQERANIALGVKHWLLAY
                     DLGRGEDIYTSYYLSQKPPYYPSHQLIKSKSEFRLIKDVSAPAYIALEPFITALPEST
                     SININTAPKQILMSLGDGLSDAQANELITARGENGITDLKEINELLKKFNIPSDQITI
                     ESQYYLSVAYAKNDEFSLVVYSLLKRSRDRKGKLLTSVIRESINNF"
     gene            complement(1355..1972)
                     /gene="lspJ"
                     /locus_tag="U6309_002423"
                     /gene_synonym="gspJ"
     CDS             complement(1355..1972)
                     /gene="lspJ"
                     /locus_tag="U6309_002423"
                     /gene_synonym="gspJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspJ family T2SS minor pseudopilin variant LspJ"
                     /protein_id="HEN5505854.1"
                     /translation="MIKNKGFTLIEILIALTVFAILATITSSTLYYAFNTRTRVNAQA
                     ERLNALQLAISIIQQDTSQTVERAIRGNEMRLFPIFVGQPQYLELTRDGVVNPNSVEK
                     RSTLERVALVCQDSKLLRRTWNTLDPIDRNIYKDKVLLDNLSECHFNYLNQNLQLLSE
                     WREQAVNQNQQREPLPKAIQMNLTLKDWGDMNMLFIIPGALYVYE"
     gene            complement(1969..2346)
                     /gene="lspI"
                     /locus_tag="U6309_002424"
                     /gene_synonym="gspI"
     CDS             complement(1969..2346)
                     /gene="lspI"
                     /locus_tag="U6309_002424"
                     /gene_synonym="gspI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspI family T2SS minor pseudopilin variant LspI"
                     /protein_id="HEN5505855.1"
                     /translation="MMPKTKLVSGFTLIEVLLALTVIAIALTALLKATAQNTDNTHRI
                     KEKTISHWIAMQGVAMIQLNLLRTSQSQESTQATTMLGQKWYWRAKISPTPIKRMQQI
                     TISVSSKQAGPFREELIAFRYLP"
     gene            complement(2343..2828)
                     /gene="lspH"
                     /locus_tag="U6309_002425"
                     /gene_synonym="gspH"
     CDS             complement(2343..2828)
                     /gene="lspH"
                     /locus_tag="U6309_002425"
                     /gene_synonym="gspH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH family T2SS minor pseudopilin variant LspH"
                     /protein_id="HEN5505856.1"
                     /translation="MTTHKGFTLIEILIVIVIIGITLGFALIAFGDFGESRRIQFSAE
                     QLANTLSLAQQQAILESSTLGLKIDNTSYQILKFQDQAGWNPISNKGIFKLYYFPKNV
                     VISLKTNQKHTRGYPAIIIAPSGDMTPFTIEFGTDKEKWIAKLTGSHNGDLDFTMAKS
                     Q"
     gene            complement(2815..3237)
                     /gene="lspG"
                     /locus_tag="U6309_002426"
                     /gene_synonym="gspG"
     CDS             complement(2815..3237)
                     /gene="lspG"
                     /locus_tag="U6309_002426"
                     /gene_synonym="gspG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspG family T2SS major pseudopilin variant LspG"
                     /protein_id="HEN5505857.1"
                     /translation="MNRQKGFSLIEIMVVVVILGILASIVVPKIMGRPDEARKVKAKQ
                     DVLAIQNALDLYKLDNGSYPTTDQGLMALVEKPTSNPVPTNWKQYLKSLPKDPWGREY
                     LYLNPGQHTDVDVFTYGAAGQPGGTGINAEIGNWDDHS"
     gene            complement(3341..4540)
                     /gene="lspF"
                     /locus_tag="U6309_002427"
                     /gene_synonym="gspF"
     CDS             complement(3341..4540)
                     /gene="lspF"
                     /locus_tag="U6309_002427"
                     /gene_synonym="gspF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspF family T2SS innner membrane protein variant
                     LspF"
                     /protein_id="HEN5505858.1"
                     /translation="MGAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQT
                     LTQQRAASSKSKVSAADLALLTRQLATLLAAGIPVEESLRGVSEQTEKDKVRELIIGV
                     RSKVLEGYGLAQAMAQYPNAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQK
                     VQQALIYPLLMIIVSTAIISFLLTFVVPKIIDVFTESGQTLPPMTQLLINLSQFIKSY
                     GFYSLVTIIVALIGFKKSLSNIKIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILF
                     AAGVSVLETMRVSSSLVTNIVMRQAFDLATLRVREGSGISEALKETKFISPMAIHLIA
                     SGEKSGQLSDMMERSASHLDNEVNRLIETSLTLLEPMVILLMGAVVLFIVLATLLPIF
                     SMEQLVA"
     gene            4637..6046
                     /gene="glnA"
                     /locus_tag="U6309_002428"
     CDS             4637..6046
                     /gene="glnA"
                     /locus_tag="U6309_002428"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEN5505859.1"
                     /translation="MTKNVVLDAIKEHDAKFVDLRFTDIRGKEQHITIPVSAVDDDFL
                     ENGKMIDGSSFKGWQKIHQSDLALVPDLTSIKPDPFFQDNTLFIRCNVVDPQTMMGYD
                     RCPRSIAQRAENYLQSTGIADGVNFGPEPEFFLFDNVQWETTISGSFYKIDSDEAHWN
                     SGKEMEGGNIGHRPPLKGGYFPVPPVDSSHDIRSAICITLESLGMVVEAHHHEVATAN
                     QCEVATRFNTLTKKADELQILKYVIHNVAHNYGKTATFMPKPLVGDNGSGMHCHQSLY
                     KDGYNLFSGDQYSGLSETALYYIGGIIKHARALNAFTNPSTNSYKRLVPGFEAPVLLA
                     YSARNRSAAIRIPHVSNPKARRIEVRFPDPTANPYLAFSAMMMAGIDGIQKKIYPGQP
                     MDKDLYDLPPEELVDVPTVCSSLEMAMDHLRADHEFLLQGDVFSRDFINNYIQMKEEE
                     ITKIRSLTHPYEFELYYSL"
     gene            6059..6619
                     /locus_tag="U6309_002429"
     CDS             6059..6619
                     /locus_tag="U6309_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEN5505860.1"
                     /translation="MNKLPLFVFLMMVICLSSAQIYKWTDSQGVVHFSDKPHQGAEKI
                     KIPEAQTYTPPANETGGSSEQEQKSVKQEDQEKYTKLEIIQPQNEATIRNNQGYVVVA
                     VQIEPDLSPGDNLQLIFDGAPLGEPQPNLIFQLNGIYRGSHTIAVQVLNDKGDVLKVS
                     DSITIFMHNPRVGMGKGRKKNINSNK"
BASE COUNT         1972 a         1234 c         1330 g         2118 t
ORIGIN      
        1 gtttaacctg ctccttctta atttctgatt tcaactcagt ggaagcggta tggtttttct
       61 tgtttctgtt tttaataata ttaatcataa caagtagtta gtaaaatggt atgttaaaaa
      121 gtaaagcgag catcaatgtt gaatctttta ctcgtaaaca gttagaaaat aaaaataagt
      181 gcatcgtgaa atccaaatga atcttatgat atctattagt atagtaaata gtttgtctgt
      241 attaaaatgc atcttctgag ggatgaaata tcagcaagaa ttccattttc aagaaactaa
      301 aagcactcat ttctggtaaa aataaaaatc agatggcaat ccattaacta agagttggat
      361 tctggcgtat tcatattgtt aggaacattt gagctcaagt caaaagttat taatactctc
      421 tctgatgaca cttgttaata atttaccctt tctgtctcgg ctgcgtttta acaaagaata
      481 cacaactaaa ctaaattcat cattctttgc ataggcaacg ctgagataat attgactttc
      541 aatggttatt tggtcactgg gaatattgaa tttttttaat aactcgttga tctctttcag
      601 atcagtgatg ccattttccc cccgagcagt tattaattca tttgcctggg cgtcacttaa
      661 tccatctcct aatgacatca atatttgctt tggggcagta ttaatgttaa tgctcgttga
      721 ttcaggcaag gctgtgataa aaggttcaag ggcaatataa gcgggtgcac ttacatcttt
      781 tatcaatcga aactcagatt tactttttat aagttgatgg ctgggatagt aaggaggttt
      841 ctgagagaga taataagagg tataaatatc ttctcctcgg ccaagatcat aagcaagcaa
      901 ccaatgcttt actcccaagg ctatattagc acgttcttgg ccggttgcct ttgagtaggc
      961 gtgacttatc aggtgcatta aagtgggaat ggattttttc tcaatcaggt tattgaggtt
     1021 gaatcgcgct tgtaaatcaa aaattccacc acttaattgt acttgattgt atatggattc
     1081 catattttta gggtattggg ctaccatccc taactggttg gtttttgtaa agcggttgtt
     1141 cttatcaagt aattcattga gagcccagaa agtaaccgcc tgagaagcaa gatatagttt
     1201 atcctgagta atcactagtc ttgttctgta aatgtcctgt tgtaatcgtg tgctcatggc
     1261 agtggcaact attgcaacca atgtcataat aaatagagcc gttaaaagag cgcttccttg
     1321 catttttgaa ctggcaatgg attttggagg tggactactc ataaacatag agtgccccgg
     1381 gtatgataaa aagcatgttc atatctcccc agtcctttaa agtgagattc atttggattg
     1441 ctttgggaag aggttctctt tgctgatttt gattgactgc ctgttctcgc cactcggaga
     1501 gaagctgtaa attttgattt aaatagttga aatgacattc agataaatta tccagaagta
     1561 ctttgtcttt atagatattt ctgtctatag gatctaaagt attccatgtg cggcgtagta
     1621 atttgctatc ctggcaaaca agtgcgactc gttccaaggt actgcgtttt tcaacactat
     1681 tggggtttac caccccatcc ctggttagtt caaggtattg aggttgacca acaaatattg
     1741 gaaacaggcg catttcatta cctcttatag cacgttccac tgtttgggaa gtatcttgct
     1801 gaatgatact tattgccagc tgaagtgcat ttaagcgttc agcctgcgcg ttaactcggg
     1861 ttcgagtatt gaatgcgtag tataaggttg aggaggtaat agttgctaat atagcaaaaa
     1921 cagtaagagc aattaatatc tcaatcagtg taaatccttt atttttaatc atggcaagta
     1981 cctgaatgca atgagttctt ctcgaaaggg acctgcttgc ttggaactga ctgaaatggt
     2041 tatctgttgc attcgtttta taggggtcgg gctaatttta gctctccagt accacttttg
     2101 tcccagcatg gtggttgctt gcgttgattc ttggctttga gaagtgcgca gcagatttaa
     2161 ttggatcatt gctacccctt gcattgctat ccaatgacta attgtttttt ctttgattct
     2221 atgcgtgtta tctgtatttt gagcagttgc ttttaaaagc gctgttaaag caattgcaat
     2281 gacagtaaga gccaataaga cttctatcaa agtaaatccg gagactaatt tagttttcgg
     2341 catcattgag attttgccat ggtaaaatcc aaatcaccat tatggctgcc tgtaagtttg
     2401 gctatccatt tttccttatc tgtaccaaat tctatagtaa aaggggtcat gtcaccagat
     2461 ggcgctataa tgattgcagg gtacccccgg gtatgttttt gatttgtttt aaggctaatc
     2521 acaacgtttt tgggaaaata gtagagttta aaaattcctt tattcgatat gggattccat
     2581 cccgcctggt cctgaaactt cagaatttgg taactagtgt tatctatttt taacccaagg
     2641 gtgctgcttt ctaaaatggc ttgttgttga gccaagctta gggtatttgc caattgctca
     2701 gctgaaaatt gtatacgtct gctttccccg aagtcaccaa aagcgattaa ggcaaacccc
     2761 aaggttatac ctataatcac tatgacaata agaatttcta tcagggtaaa acccttatga
     2821 gtggtcatcc cagttaccaa tttccgcatt aatccctgtt ccgccaggtt gtcctgctgc
     2881 cccataagta aagacatcaa cgtcagtatg ttgtcctggg tttaaataaa gatattctct
     2941 gccccatgga tcttttggca atgatttcag atattgtttc caattcgttg gaacaggatt
     3001 gcttgttggt ttttctacca gagccattaa accttgatca gttgtagggt atgaaccatt
     3061 atcaagtttg tataaatcga gtgcattttg aatggctaaa acatcttgtt tcgctttgac
     3121 tttacgggct tcatccggac gtcccatgat cttggggaca actatagatg ccaatatgcc
     3181 taaaatcacg acaacaacca taatttcgat taaagaaaag cctttttgtc gattcatttg
     3241 tgtacctctt gaaactaaat gttaaattat aacacaaata ttgatgccta tgaatagact
     3301 taaattttaa aaggactcgg atagcccttt ctccgcctaa tcaggcaact agttgttcca
     3361 ttgaaaaaat aggtaataaa gtcgctagta caataaataa aacaacagca cccattaata
     3421 gaataaccat aggttctaat aaagttaagg atgtttcaat cagtcggttt acttcattat
     3481 ctaaatgaga cgctgagcgt tccatcatgt ctgaaagttg gccgcttttc tcaccactgg
     3541 ctatcagatg gatagccatg gggctaatga attttgtttc tttgagggcc tcactgattc
     3601 cacttccttc acggactcgt agagtagcta aatcaaaagc ctgtctcatc acgatattgg
     3661 ttaccaaact tgaagaaaca cgcatggttt caagtacact gactcccgct gcaaatagaa
     3721 ttccaaacgt gtgaatatat cgcgcgacat taattgtttt aaccaaataa gacacgatag
     3781 gtagtttaag catgaattga tgccatgctg ttttaatttt tatattactg aggctctttt
     3841 taaatccaat tagagccaca attattgtca ctagactata aaaaccataa gatttaataa
     3901 attggctcaa gttaataagc aattgcgtca ttggtggtag tgtttggcca ctttcggtaa
     3961 acacatcaat aattttaggg acaacaaaag taagtaaaaa actgataatg gcagtagaaa
     4021 caatgatcat gagcaatggg taaatcaagg cttgctgcac tttctgtcga gtttgttgtt
     4081 gtttttcagt ataatcagct agtttttcta aaacaacatc aagacgccct gtctgctcgc
     4141 ctgagcctac tgttgcccgg tatagttctg gaaaggcatt gggatattga gccattgcct
     4201 gagcaagacc atacccttcc aatactttag agcgaactcc gataataagc tctctgactt
     4261 tgtctttttc tgtttgttcg ctgactccgc gcaatgattc ttcaacagga ataccagcag
     4321 ccagaagtgt tgccagctgt ctggtgagta atgccaaatc agccgcagaa actttacttt
     4381 tgctgctggc agcacgttgc tgggttaatg tttggacctg agtaggaatt agtccttgtt
     4441 ctcgcaataa ttgacgtgca tgacgctcgg aatcggcttc tattacacct ttgctaatgc
     4501 tgccattcac ttttagcgct tgatattggt aggcgcccat attatttttg ttaaaattaa
     4561 aactatagag tgtaatctat tgggattaat aagtcactta aggtaaacta tacccggact
     4621 atcctggaga caaaagatga caaaaaatgt agtgcttgat gcaatcaaag aacatgacgc
     4681 caaatttgtg gatttaagat ttacagatat ccgtggaaaa gagcaacaca ttaccatacc
     4741 tgtttcagca gttgatgatg attttcttga gaatggaaaa atgatcgatg gctcatcttt
     4801 taaaggttgg cagaaaattc atcaatcaga tttggcttta gtgccagatt taacttcaat
     4861 taagccggat ccattttttc aggacaacac tttatttata cgatgtaatg tagttgatcc
     4921 acagactatg atgggttatg acagatgccc acgttcaatc gcacaacgcg cagaaaatta
     4981 tttacaatct acagggattg cagatggtgt aaattttggc cctgagcctg agttttttct
     5041 cttcgacaat gtgcaatggg aaactactat cagcggttca ttttataaaa tagattcaga
     5101 tgaggcacat tggaactccg gtaaggaaat ggaaggcgga aatattggcc atagacctcc
     5161 gttgaaaggc ggttatttcc ctgttcctcc agtcgattca tctcatgata ttcgttcagc
     5221 aatttgtatc acattggaat ctttaggaat ggttgtggaa gcacatcatc atgaagtagc
     5281 tacagctaac cagtgtgaag tagctacccg ttttaataca ctcaccaaaa aggcagacga
     5341 actgcaaatt ctgaaatacg tgatacataa tgttgcccat aactatggga aaaccgctac
     5401 ttttatgcca aaacctttgg taggagataa tggaagcggg atgcactgtc atcaatcact
     5461 ttataaagat gggtataacc tgttttccgg tgatcagtat tcggggcttt ctgaaacagc
     5521 tttatattac ataggtggaa taatcaagca tgccagagct ttaaacgctt ttaccaatcc
     5581 gtcaaccaat agctataaga gattagttcc cgggtttgaa gcgcctgtat tactggctta
     5641 ctcagcaaga aatcgctccg ctgctatccg catcccacac gttagcaatc caaaggcacg
     5701 acgtatagaa gtacgtttcc ctgatccaac agctaatcct tatcttgctt tctcagcaat
     5761 gatgatggca ggcatagatg gtattcagaa aaaaatctac cctgggcagc caatggataa
     5821 agatctttat gatttaccgc ctgaagagct tgttgatgta cccactgttt gttcttcatt
     5881 agagatggct atggaccatt tgcgggcaga tcatgaattt ttgttacagg gagatgtgtt
     5941 cagcagagac tttattaata attatattca aatgaaagaa gaggaaatta ctaaaatcag
     6001 gagcttgact catccttatg aatttgagtt atattatagt ctctaagggt ctatattcat
     6061 gaataaactg ccactattcg tctttttgat gatggtaatt tgcctttcca gcgcgcaaat
     6121 ttacaaatgg acggatagtc agggtgttgt tcattttagc gacaaacctc atcagggtgc
     6181 tgagaaaata aaaattcctg aagctcaaac ctatacacca cctgcaaatg aaacaggtgg
     6241 ttcttctgag caagaacaaa agagtgtcaa acaggaagat caggaaaaat ataccaaatt
     6301 agaaattatt caaccgcaaa atgaagcaac aattcgtaat aatcaaggtt atgttgttgt
     6361 tgcagtccaa atagaacctg atttgtctcc aggggataat ttgcagctta tttttgatgg
     6421 ggcgccttta ggagagccac agcccaattt gatttttcaa ttgaatggta tctatcgagg
     6481 atctcataca attgctgttc aggttcttaa tgataaaggg gatgtgctta aggtcagcga
     6541 cagtattaca attttcatgc acaatcctcg cgtcggtatg ggcaagggca gaaagaaaaa
     6601 tatcaattct aataaataga gcaggttatg tatgttaact ttgttatcgc cagc
//
LOCUS       DASLOE010000157         6644 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.158, whole genome shotgun sequence.
ACCESSION   DASLOE010000157 DASLOE010000000
VERSION     DASLOE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.158"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            61..2370
                     /locus_tag="U6309_002430"
     CDS             61..2370
                     /locus_tag="U6309_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505861.1"
                     /translation="MADSRSSIQSILEIDKRFDENYYNYLVNDNYLIPAEAAVIANGL
                     VIFKEKFESGSLFMQRKDNHDSFTHEQILQYYLELALTCGRLDIIKYLIDELHVSLSE
                     EKIFHFLFLHSPYEGNKYSHNIVEIYNYLKNEKEIFSEKALIEEIFLAEAKKGHIQAV
                     KAIIELKLLPNINITDEAGHTAFFYCILESKKKRFSFNPEELGAEDVILSVRDKEILG
                     LIELVNAAILAMEDKNEEKLVKIRKALADSIGRVPETLIEDCLSRAIELNDVKMIQLL
                     LPVCNPDLKFLMLGKANSVEAGMLLIEHFISTGFVIPEARSLEGYAHRLGLSEYSAGA
                     EQANRFLLHAVAGFVNRTDNGKDFNTILRALQKTVLLNGIANVEVARKILYQTYLEGE
                     PLLLTVGWTQHGVGLSILWDKESNKTYLVFSNRGQGGIASCLNTKGLIKKEEPKAGSV
                     IYEIDGKLDEGFFSHFEIGENKLGLFRYGKKAFEKDIRTCLKDARVLPHFIFAAAQNY
                     ATCGYVNTKQSIRGLLCVFAMTRGDSLNEELIDRMSRQYKEFTAHDRNVACSELIEDF
                     QRKIKFDSRHIQKEYQDFIVKLILSHCTSDKPEDIERTQRLFVVLPEEIQEKLAHLIP
                     CHIVARKEDKSSTLQNLSLFKHQEEPSSQNDYFLHRPIGVIPDSIDNLLNKYHEMNTL
                     LKFLESHGYTEKDIPTIVLENNSVRIHTKSDRESKYKVKDLFPKDIQIINVQGRYEFI
                     LKDLTEVSYVLNKLNGQTPENNSNPDFGL"
     gene            2500..3981
                     /locus_tag="U6309_002431"
     CDS             2500..3981
                     /locus_tag="U6309_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEN5505862.1"
                     /translation="MNAYQRLEQHFKKYYDFENLAAIVSWDEATMMPVGGGQARAEAL
                     ATLSAVQHEWLTNKKIGDWIKQAKDLPGLSLWQRRNVYWIDKQYQQATCIPAQLIEEF
                     TNESLTCVQAWRELRAKNDWHSFKPYLKKLFEKVKTIADIKSQRFNKPCLDVLIDEYS
                     PDLDCKAITPVFNVLKQEIPVIIPNVIEKQKTRDIKVIAGDFSAEKQKQLGIEIMSRL
                     GFDFNRGRLDVSHHPFCGGISDDVRMTTRYSEQEFISSLMAITHETGHALYEQGLLKE
                     WSRQPVGHSLGMSVHESQSLLIEMQICRSLEFISFLVPIAKKYFKEAQNITEENLYFH
                     CTKVQPDLIRVEADEVTYPLHVILRYEIEQLLFSSEITIDDLPEVWDNFMRKYLGLST
                     KGNDKNGVMQDVHWPAGNFGYFPAYTFGALIAAQLFSAAKKAIPDINDQIKSGDFQSL
                     LIWLRENVHQKAKLFSYQELLCQATGEELNPQYYLAHIKQRYL"
     gene            4042..4545
                     /locus_tag="U6309_002432"
     CDS             4042..4545
                     /locus_tag="U6309_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505863.1"
                     /translation="MLFFSSAMSKHKLTFTKLSNCMKYLDLAATWAEEEWGYIRNKGV
                     EFRKKILSDLKEHTYIGIFNGQPVAMFVLFEKDMSPEFNYGRLKPPVVSELMYVYIDK
                     PYRGLGFGKQVIDEAKQLASHLKSEYIMLDTLKPGLNRFYEKGGAKVIAENQLFLHPT
                     DVLTIKI"
     gene            4839..6353
                     /locus_tag="U6309_002433"
     CDS             4839..6353
                     /locus_tag="U6309_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEN5505864.1"
                     /translation="MYMDFGSDNMALSKEIQNQSLRSLIQKYARQLNETDKKLIELIN
                     NLAAADDIPLYAQNQNVINLLIKTIKDSQSEHSPDLEGTSYFIYGLITAEMEAQSEIN
                     RQRHLMQGRFDETALHLRTERDQMQDHDMTLLMPKSQGRIVVMAVLNRYDSHSANAII
                     ETLASDVFNPEVHYIMIPVGPGHWRGVYLSKPQRGISDTVYDLELFDPYGPAGAAVLD
                     DYVLDLLNQCGVPKELVNIRHTGPKHPQGDAYSCGDFTCAYSHKKMKEFGASEGSYNP
                     ILIDTLDNLGNEDNVLRMATREETRALVDKDRRRQHINPAIHSLRGADKEEPVSSIGK
                     PKAGAHGISFEIENANKSKKATIRQTVKESQLSPAMRTGLIIGGGTFTSGGVGAIIGG
                     LMGFFLLPGLGVLMGAIAGAFIGAGAGLLISGIIANAFWNSTPLNIIKTHESNTEIQG
                     VDLKSDSAGKPDKECSEKDEEPIHSPPLFFTPATQERAANDMVDELNSNGPKTG"
BASE COUNT         2179 a         1067 c         1379 g         2019 t
ORIGIN      
        1 gcctctcgtt agtcattttt tgattatact taaaaataac aatcaaaagg agatggcgtt
       61 ttggctgatt ctagatcatc gatccaaagt attttggaaa ttgataaacg atttgatgaa
      121 aattattata attatttggt taatgataat tatttaattc cagcagaggc cgcagtcata
      181 gccaatggat tagtaatatt caaagaaaag tttgagagtg gaagtttatt catgcagaga
      241 aaagacaatc atgactcttt cactcatgaa caaatccttc aatattattt ggaattggct
      301 cttacttgtg ggcgtcttga tatcattaaa taccttattg atgaacttca tgtttcctta
      361 tccgaggaaa aaatatttca ttttctattt ttacacagtc cttatgaagg gaataaatat
      421 tctcataaca tagtggaaat ttataattat ttgaaaaatg agaaggaaat ttttagcgaa
      481 aaagcgctga ttgaggagat atttttggct gaagctaaaa aaggtcatat tcaggctgta
      541 aaagccatca tagaacttaa gcttttaccc aatatcaata ttaccgatga agccgggcat
      601 acagcatttt tttactgtat tttggaaagt aagaaaaaga gattttcctt taatcctgaa
      661 gaattgggag cagaggatgt cattttatca gttcgagata aagaaattct tggattgatt
      721 gagctggtaa atgcagctat tttggcaatg gaagataaaa atgaagaaaa actggttaaa
      781 ataaggaaag cgctcgctga ctctataggt agagttcctg agacgttgat agaagattgt
      841 ttgtcgcgag caattgagtt gaatgatgtc aagatgattc aacttctgtt acctgtttgt
      901 aatcctgatc taaagttctt gatgttaggt aaggccaatt ccgttgaagc cggtatgcta
      961 ctaatagagc attttataag tactggattt gttatacccg aagcaaggag tttggagggg
     1021 tatgcgcaca ggttagggct gagcgaatac agtgctggtg ccgaacaggc aaatagattt
     1081 ttgttacatg cagtagctgg atttgtcaat cggacagaca atggtaaaga cttcaatact
     1141 attttaaggg cgctacaaaa gacggtgcta ttaaacggca tagcgaatgt ggaggtggcg
     1201 cggaaaattt tatatcaaac gtatttggaa ggtgaacctc tattgcttac ggtgggatgg
     1261 actcaacatg gtgttggttt atccatatta tgggataaag aatcaaataa aacttatctt
     1321 gtattttcta accgagggca aggaggaatt gcttcatgtc ttaataccaa gggtttaatt
     1381 aagaaggagg agccgaaagc aggttcagtt atctatgaga tagatggtaa gttggacgag
     1441 ggctttttct ctcattttga gataggggaa aataaattgg gtctttttag atatgggaag
     1501 aaagcatttg aaaaagacat caggacatgt cttaaagatg cccgagtatt accccacttt
     1561 atttttgctg cagcacagaa ttatgcaact tgtggctacg tcaacacaaa acaatccata
     1621 cgcggtttat tatgtgtttt tgctatgaca agaggcgatt ctttaaatga agagttaatc
     1681 gatagaatga gtcgacaata caaagaattt acagctcatg atagaaatgt tgcttgttcc
     1741 gagttaattg aagattttca gaggaaaatt aagtttgatt ccaggcatat tcaaaaggag
     1801 tatcaagatt tcatagtcaa attgatttta tcgcattgta catcggataa accagaagat
     1861 attgagcgaa cccaacgatt atttgtagtg ttaccagaag agatacagga aaaactggct
     1921 catctgatac cttgccatat cgtagcacgg aaagaagata agtcatcaac ccttcaaaat
     1981 ctgagtctct tcaaacatca ggaagaacct tcctcccaaa atgactattt tttgcatagg
     2041 ccgatcggag taatccctga ttccatagat aatttactga ataaatatca tgagatgaat
     2101 acactactta aatttttaga atcccatggt tatacagaaa aagatattcc gacaattgtt
     2161 ttggaaaaca attcagtccg tattcatact aaatcggata gagaatctaa atataaagta
     2221 aaagatcttt ttcccaaaga tatccagatt attaatgttc agggaagata tgagtttatt
     2281 ctcaaggatc tgacggaagt aagttatgta ttaaataaat taaatggcca gacaccagag
     2341 aacaatagca acccggattt tggtctataa tattttttca tgagtcagtt tttaaatttc
     2401 ttgaaaggat tttaactatg ttattctgta tgtggtgctt tgaaagttat cagatctgag
     2461 tggagcccat atttttaaag tataagccaa gagtttttca tgaatgccta ccaacgatta
     2521 gaacaacatt ttaaaaaata ttacgatttt gaaaatttag cagccattgt ttcctgggat
     2581 gaagcaacga tgatgccagt cgggggtggt caggcgcgtg cagaggcgtt ggccacttta
     2641 agtgctgtac aacatgaatg gttaactaat aagaaaatag gtgactggat aaaacaggca
     2701 aaagatttac caggtttatc attatggcag cgacgaaatg tctattggat tgataagcaa
     2761 tatcagcaag caacctgtat tccagcgcaa ctgatagaag agtttacgaa tgaatctttg
     2821 acatgtgttc aggcatggcg ggaattacga gctaaaaatg attggcactc atttaaaccg
     2881 tacttaaaaa agttatttga aaaagtaaaa acgattgctg atattaaatc acaaagattt
     2941 aataagcctt gtttggatgt cttaattgac gaatattctc cggatttgga ttgcaaggcc
     3001 atcacccccg tttttaatgt cttgaaacaa gaaataccag tcattattcc caacgttatc
     3061 gaaaaacaaa aaacgcgaga tattaaagtg attgcaggtg atttttctgc ggaaaaacaa
     3121 aagcaacttg ggattgaaat catgtctcga ttggggtttg actttaatcg cggccgatta
     3181 gatgttagcc accatccgtt ttgtggtggt atttcagatg atgttcgaat gacaacccga
     3241 tatagcgagc aggaatttat atcttcctta atggccatta cccatgaaac aggtcatgct
     3301 ctgtatgagc aaggcttgct aaaagaatgg agtcggcaac ctgttggcca ttcattggga
     3361 atgtctgtgc atgagagcca atcgttgctt attgagatgc aaatttgtcg aagcttggaa
     3421 tttataagtt ttttggttcc tatcgcgaaa aaatatttta aagaagctca aaatataact
     3481 gaagaaaatc tttattttca ttgtacgaaa gtacaacccg atttaattcg tgttgaggca
     3541 gacgaagtca cttatccact acatgtaata ctgcgttacg aaatcgaaca acttttgttt
     3601 tcttcagaga ttactattga tgatttacca gaggtctggg acaacttcat gagaaaatat
     3661 ttaggattat caacaaaagg gaacgacaaa aatggcgtga tgcaagatgt gcattggccc
     3721 gctggaaatt ttggctattt tcctgcttat acgtttggtg cattaattgc cgctcaattg
     3781 ttttcggcgg ccaaaaaagc aatccctgat atcaatgatc aaatcaaatc aggtgacttt
     3841 caatcgttgt taatttggct tagggaaaat gttcatcaaa aagcaaagtt attttcttac
     3901 caagagttat tgtgtcaagc aacaggcgaa gagttaaatc cgcaatatta cttggctcat
     3961 atcaaacaga ggtatttata aaacaggatg gtcttggata aaatttgcag gttgtatcat
     4021 tttaatcaag agggttatca gatgttattt tttagttcag caatgtccaa acacaaactc
     4081 acttttacta aactttctaa ttgcatgaaa tatttggatc ttgccgcaac atgggcagag
     4141 gaagaatggg gatatattcg taataaagga gtagagtttc gtaaaaaaat cctgtctgat
     4201 ttgaaagaac atacatatat aggaattttt aatggacaac ccgttgccat gttcgtttta
     4261 ttcgaaaaag acatgagtcc ggaatttaac tatggaagat tgaaaccccc ggtagtaagt
     4321 gagttaatgt atgtctatat tgataagcct tatagaggac ttggttttgg aaagcaagtt
     4381 attgatgagg cgaaacaatt agcaagccat ttaaaatcgg aatacattat gttggatact
     4441 ttaaaaccgg gcctgaatcg attttatgaa aagggcgggg caaaagtgat tgcagaaaat
     4501 caattgtttt tgcatccaac tgatgtattg acaataaaga tataagctgt gctctctatc
     4561 acgggtaagt aatgatcttt tttatgtgga cataagcatg ttatcgtgtt taagtaatca
     4621 tatattcaat gggtgcaata attaatcatg agagtaaatt tcttttcggt taattatttt
     4681 ttatttcatt agacctcttg ccctaaaaca aaagagacag gttaggcaaa aggtctaatt
     4741 tagagccaaa agcattgtcg aactgcttca gataggggca tacattatta gagaggaggg
     4801 aagaggcttc attttttctt aattgtttta tatagcatct gtatatggat tttgggagcg
     4861 ataatatggc attaagtaaa gaaattcaga atcaaagttt aagatcatta atccaaaaat
     4921 atgccagaca acttaatgaa acagacaaga agctcataga acttattaat aatctcgctg
     4981 cagcagatga tattcctttg tatgcccaaa accaaaatgt tatcaatttg ttaattaaaa
     5041 ccattaaaga cagccagtct gaacacagtc cggatttgga gggaacttcc tatttcattt
     5101 atggattgat cactgcagaa atggaggctc aatctgaaat aaaccgccaa agacatctta
     5161 tgcagggacg ttttgatgaa acagcattgc atttaagaac agagcgtgat caaatgcaag
     5221 atcatgacat gactctgctc atgccaaaat ctcagggtcg tatcgttgtg atggcagttc
     5281 tcaatcgcta cgactctcat tctgctaatg cgattataga aactttggca agtgatgtat
     5341 tcaatcccga agttcattac attatgatac ctgttggacc cggacattgg cgaggagttt
     5401 atttaagcaa accccaaagg ggcattagcg atacagttta tgatcttgaa ttatttgacc
     5461 cttatgggcc tgcaggcgct gctgtacttg atgattatgt acttgattta ttaaaccagt
     5521 gtggcgttcc caaagagctt gtgaatatac ggcatacggg tccgaaacat ccgcaaggtg
     5581 acgcttactc atgtggggat tttacttgtg cctatagcca taagaaaatg aaagaatttg
     5641 gagcttcaga aggtagttat aatccaattc ttatcgatac actggataat ttaggtaatg
     5701 aagataatgt acttcgcatg gcaacacgcg aagaaacgcg agcgcttgtt gataaagaca
     5761 gaagaagaca gcacattaat cctgctatcc attctctgcg gggggcagat aaagaagagc
     5821 cagtatcgtc tattggcaaa cccaaagcag gtgcacacgg gatatccttt gaaattgaaa
     5881 atgctaataa atcaaaaaaa gcaactattc gccaaactgt taaagaaagt caattatcac
     5941 cggctatgcg aacaggtctt attataggtg gaggtacatt cacctcagga ggagtagggg
     6001 ctattattgg cggactcatg ggttttttcc ttttgccagg actgggtgta ttaatggggg
     6061 cgatagcggg agcttttatt ggtgcaggag caggtttgtt gatatcaggt attatagcca
     6121 atgcattttg gaatagtact ccattaaata ttataaaaac ccatgagagt aacaccgaga
     6181 ttcaaggagt cgatttaaaa tccgactctg cgggtaaacc tgataaagaa tgttcggaaa
     6241 aggacgagga gcctatccac agtcctcctt tatttttcac tcccgctacg caagagagag
     6301 ctgcaaatga catggtcgat gagttgaatt caaatggccc taaaaccggg taaacatcag
     6361 tgaaatattt cattcatctt tgcatcttgc aaataaacaa tagcagtcga gctcattctg
     6421 ttttacaaaa gcttcaggaa ccatgctgta aaaatttcaa ttcagcattt gtagatattc
     6481 ttttaattat gggattaatg tgctaatatg agtcatttaa tataaatact gtccgaaaca
     6541 tttcatgact ctgcaaaaat acttccctca gttcaatgag gcatggcaat cttactcact
     6601 ttgtcgctct tcgctgtggt ggcctttaag ctatcttcta ttaa
//
LOCUS       DASLOE010000158         6601 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.159, whole genome shotgun sequence.
ACCESSION   DASLOE010000158 DASLOE010000000
VERSION     DASLOE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.159"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(233..1342)
                     /locus_tag="U6309_002434"
     CDS             complement(233..1342)
                     /locus_tag="U6309_002434"
                     /inference="COORDINATES: protein motif:HMM:NF016847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEN5505865.1"
                     /translation="MSNTPHTQNGLLACTSMEYARHRPEQTLLYQVIGEYYPAFLSHL
                     AELEKSVPQYVQDEFESYLKCERLEHGFLRVQCESYHVEQLVAFSCKRRGFCPSCGAK
                     RMVESAALLVDEVLPEKPIRQWVLSVPYPLCWLFASEPEMMSKALLIVTRAISSYLIK
                     KTGFTHKTAKTGAVTLIQRFGSALNLNIHFHMLFLDGVYGVDSGGAIGAFHGIKPPSS
                     KEMSLLLGKISERIARLVEQAGYLERDEGDGLKLEGFEDEIMNHFQGSSITYKIAVGS
                     QKGRKVLTIKTLPCEGSKMSWAMRLKRVFNIDITVCRHCQGRVRIIACIEEQVVIDQI
                     LAHMNQQQQSRILVQVSSCIRTPPIEQSSQMTMNY"
     gene            1998..2805
                     /locus_tag="U6309_002435"
                     /pseudo
     CDS             1998..2805
                     /locus_tag="U6309_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844225.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2906..3178)
                     /locus_tag="U6309_002436"
     CDS             complement(2906..3178)
                     /locus_tag="U6309_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN5505866.1"
                     /translation="MNKKLSRLQLLNEFESAPASALFNQQTLAAVLDCSTQLLERNRW
                     EGKGIPYLKIGRKVLYRKSDVLSFLQQQKIYRSTCDEGQVLSVANQ"
     gene            3748..5028
                     /locus_tag="U6309_002437"
     CDS             3748..5028
                     /locus_tag="U6309_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN5505867.1"
                     /translation="MFERKLTPIITELLAEFRIVYLTGPRQAGKTTLTKIIAQNNGLE
                     YISFDNQTILQSAQNDPIGFINSLHNKKVVLDEFQYIPELIPAIKQASDNLPPNVKGK
                     FLLTGSADIFRSGKTQEALPGHMARLELYPLSLSEQYKTDYNIVDLLCNENFNLNNLT
                     LLTRADLANFILKGGYPEIQDKSSRAKQIWYKSYIEGRLFKDFETLYHAKGDYRSKLE
                     ALIPYLAGISSNLLKYSNISNDLGEDDKLVKAYIEVLELMFIIKRVPAYLKNKAKRQA
                     ITIPKLQTIDTGLACSLLGIKNEGQLLNSPHYGGLLENLIYMELLKQNSWSEEKVELL
                     HFRDKYQNEVDIVMERDNHQIIGVEIKASATVNIHDFKGLIKLAEFNPSKFQYGVIFY
                     SGKEILPFSKNEIKLFALPISLFIKSDTKKDDVQ"
     gene            5302..6453
                     /locus_tag="U6309_002438"
     CDS             5302..6453
                     /locus_tag="U6309_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HEN5505868.1"
                     /translation="MYPFILKPKSLRFFNLVFIALTLSFCLLANAQAAGIERYDFGDM
                     ASKVTKAWDQDVALYVAKRFAVENSFDEKEITVKKLNAGATNHFIYLMFINNKLKFVI
                     KGLGTEKEAEALFALQNYSKIPEIQNNPQAATVTLSKIINSYRVKNDSRTYFFAILEA
                     AQGKELFKIMTYELLQHKDINTLNDVFYRIGQQTGELHKTIIGSDQFITPRDLVTVIH
                     DDFHPENTFYNLETNIFSLIDNESMGDSITEPLPIIRELYGFYEVPIIRWPSEKITVD
                     LLKDADPHDIASIYYDFVLGMASVYQDPQDAKRVLIEAIIDLNNMAIAFLKDRYSEPW
                     MLNPSDKHEAHIVWGKLAKVDTNPQLAELYITKLTIINNVLQSLSGFKR"
BASE COUNT         2097 a         1325 c         1182 g         1997 t
ORIGIN      
        1 attagggtat aggaatttta aaccacctat ttcccatttt tcaaaagtat cctatttaaa
       61 ttcttagcaa gaaaccaccc atttttatta ataaacaggc tgcattcatg aacaagctcc
      121 cactagatcc agaaaacacc tcattttgat gtttgattta tgctcatttg taaatccaag
      181 atgacagcaa gcctcatgct gattccatgt tcttttgatt tgactaaagt cactaataat
      241 tcattgtcat ttgacttgac tgctcaattg gcggtgtccg gatacaagat gaaacttgga
      301 ctaatatccg cgattgttgc tgttgattca tatgcgccag aatctggtca atcaccactt
      361 gctcttcaat acaagcaatg attcttaccc taccctgaca atgcctgcaa accgttatgt
      421 caatgttaaa cactcgcttt aaccgcatcg cccaactcat ttttgaaccc tcgcatggca
      481 aggtctttat cgtcaatacc ttccgtcctt tctgggaacc caccgcaatt ttgtaggtaa
      541 ttgaactgcc ttgaaagtgg ttcattattt catcttcaaa accttccagt ttcagaccat
      601 caccctcatc cctctctaaa tagcctgctt gctcaactag tcttgctata cgctcactaa
      661 tcttgcctag caataaactc atctctttgc tgctaggtgg tttaatcccg tgaaacgctc
      721 caatggcgcc tcctgaatca actccataca ccccatccag aaataacatg tgaaagtgaa
      781 tattcagatt caaagcactg ccaaaacgtt gaatcaaagt cacagcacct gtcttggctg
      841 tcttgtgagt gaatccggtt ttcttaatca aatagctact gatagctctt gtcactatta
      901 atagtgcctt actcatcatc tcaggctcgc ttgcaaataa ccaacataga ggataaggca
      961 cactcaatac ccactgacgt attggctttt ctggtaatac ttcatccact agcaaagcag
     1021 cgctctccac catccgtttt gccccgcagc tcggacaaaa acctcggcgc ttgcaactaa
     1081 atgcaaccag ttgctcaaca tggtaagatt cgcattgtac acgtaaaaat ccatgctcaa
     1141 gtcgctcaca tttcaggtag gactcaaatt catcttgaac atactggggt actgactttt
     1201 ctaattcagc aagatgggac aaaaatgcag gataatactc cccaatcact tgataaagta
     1261 aggtttgctc gggtcgatgg cgtgcatatt ccatggaggt gcacgccaat agcccatttt
     1321 gggtatgggg agtgttggac ataggttgct tcgctttcca ttgcgtacct caacctaaca
     1381 cataaattta tttaacaaca agtcctcaat tcattagaat gcttttctgc gtcactgcaa
     1441 ttttccagca gtgacgcagg aatattcgcc atcactaccc ctccaacacc aagccttaaa
     1501 acaccatttg tcctgacgca ccagataaga taggacaaaa tattccctga aatggtattg
     1561 ctcccagatc tgcactttgc tattttctaa atgaagcatg actgatgaat cggtgaaaat
     1621 ccgatgaaac gcttcggcgt cttatgctaa gccactctca ttccctaacc ttgtggctgg
     1681 acgtatagtc gggaacccct caggaacctt taccgttcgg taaagggagt tcttcggaac
     1741 tctaagtcat ttcaattgac catggattag agataacgct ctgctttatc tcttgaattt
     1801 atttatttga ttgaggagaa aaacacaaaa aaactagcag tacaggcagc agtatctttg
     1861 ccttataacg attgatttgc ccccttcttc gagctttacg gtttttaccg ggtttcaaca
     1921 gagagtaaac cgctcgacca acactaaaca ctacacaggg caatatattt ttttaacaca
     1981 tttttgcgga taggaagatg cgtacacaat ccttaagaca attcgctaat cgacaaatca
     2041 aacagggtag gcaaggtaaa tatctgtatc ggaaacaccg tgcctatatc atccataaga
     2101 tgattgatga cttgtttgtt attcggcgaa tgccgccgtc atggcaagct ttgcaatctg
     2161 agcaggttca caagctggtg cgttactgga aaaaagaata ttaatcccgt caccatcatg
     2221 cgctacatga ccattattca tcgttttttg cagatgaaca attgccctgt tgctaatatt
     2281 gataatcaat cgcttgaact cttaagacct aaaacaagaa aaaagagaaa gaaaacaatc
     2341 cctcctgata tttggaagtc acttcacgac ccaatagccc gagtcatcat gggattacaa
     2401 acagagtttg gactcacttt caaagaagcg attctgatta aaccaggtat tcaagtccat
     2461 gaatgttcta tcgggataac ccgagacatt gcttttaatt ccaccgaccg aagcataccg
     2521 atacgaactg aaaaccagaa agcggtactc aatcttttta actggctcac aaaacaatac
     2581 ggtaatctgc ttcaattaaa atcatatgag gaaacccgct ttatctggcg cagtgcattg
     2641 gcaaagcacc gtttatccag cagcaaaagc tggcgatatt tgtatgccaa acaaatgtac
     2701 tctcaccttc tacctgacca tggaaattac aagacgtgtt ggctgatacg tgatgaaatg
     2761 ggaataaaat cgcggaatac tctgtggtta tatctcaaaa attagcggga catttgggac
     2821 atgcgggaca gcgcatacac acggggttcg agccgtccca atttgtcgca tcacttaaaa
     2881 ataatgggac aaatttccat ctgttttatt ggttggccac tgaaaggact tgcccttcgt
     2941 cacaggtaga tcgatagatt ttctgttgtt ggagaaaaga caatacgtca cttttgcgat
     3001 acaaaacctt acggcctatt ttaagatagg gaatgccctt tccttcccaa cgattacgtt
     3061 ccaaaagttg ggtagaacag tctaaaacag ccgccaatgt ttgttgatta aacagcgcag
     3121 aagctggtgc ggactcaaat tcgtttaaca gttgtaaacg agataatttt ttattcatat
     3181 agaacctcat caaatgatta ttcattcagc ttaatgccga cggtttgcgt cctgctcgat
     3241 gaatatcagt tgactgaccg gccggataat agtcgtgata tttcattttc cgtgactttt
     3301 acaggaccgt ggacccgtaa aagtttacta ctcgcgagaa gtattgcttg caatttaaaa
     3361 gggtgtcaag cgctttaaag ccttgtgatc tgattgttat taaatgatat tcattgttac
     3421 taaaaaaatt tagccagaga aatttgtccc attcgtccca agtgaaaaac tgatattggg
     3481 acacagaaaa ccgagtgcct atgcggttgt cccaattgtc ccgaatgtcc cacctaattt
     3541 tttataaagt gctttaaata aaatttaatc taattttaag ctcaacaata aagattccca
     3601 tatggattat gcggaatatc accttggatt tctcgggtta tcatattgga taattcggat
     3661 tttcatactg gataattcgg gtttttatta gctatttttt aagagatgtt atatattgaa
     3721 aacatgttac ctattatatg aatcccgatg tttgaaagaa aattaacccc tattattact
     3781 gagttactag ctgaattcag aattgtttat ttaaccggac cacggcaagc aggcaaaaca
     3841 actttaacaa aaataattgc ccaaaataat ggccttgaat atatttcctt tgataatcaa
     3901 acaattctcc aatcagctca aaacgatccg attggtttta taaattcttt gcataataaa
     3961 aaggtcgttt tggatgagtt tcaatatata cctgaattaa ttccggccat aaagcaagca
     4021 tcagataacc tgccacctaa tgtgaaagga aaattcctgt taaccggatc agcagatatt
     4081 tttcgatcag ggaaaacaca agaagcttta cctggacata tggcgagatt agaactatat
     4141 ccactatcat tatcagaaca atacaaaact gattataata ttgtcgatct attatgtaat
     4201 gaaaatttta acttaaataa cctaacgcta cttacccgcg ccgacttagc caattttatt
     4261 ctcaaaggtg gatatcccga gattcaagat aaaagctcaa gagcaaaaca aatatggtac
     4321 aaatcttata tagaaggtcg tctttttaaa gatttcgaga ctctatatca tgctaaaggt
     4381 gactatcgtt ctaaacttga agcccttatc ccttatttag cgggaatctc gagcaatcta
     4441 cttaaatatt caaatatttc aaacgactta ggtgaagatg ataaactggt caaagcatat
     4501 atcgaagttt tagaattgat gtttatcatt aaaagagtcc ctgcttactt aaaaaataaa
     4561 gcaaagcggc aagctattac aatacccaaa ctgcagacaa tagatacagg acttgcctgc
     4621 agccttttag gtataaaaaa tgaagggcaa ttacttaata gccctcatta tggagggcta
     4681 ttagaaaatt taatttatat ggagttgctc aaacaaaaca gttggtcaga agaaaaggta
     4741 gaattattac atttccggga taaataccaa aatgaagtgg acatcgtcat ggaaagggat
     4801 aatcatcaga tcattggagt tgaaatcaaa gcgtcagcaa ctgtaaatat acacgacttt
     4861 aaaggattaa taaaattggc agagtttaat ccttcaaaat tccaatatgg tgtcattttt
     4921 tattctggca aagaaatatt acctttctct aagaatgaaa ttaaattatt cgccttacca
     4981 ataagtttgt ttatcaagtc ggatacaaag aaagatgatg ttcaataaaa atagtgcata
     5041 tctaaaaaac aagagcctcc aaagaggctc tgtgtcctgg aaacaagtcc caggggaagt
     5101 tgtaacataa ttatacgcat gtatttctac tttccaggaa tccagagttt tcccatgagc
     5161 ctttagagcc ttaaaggcgc ttttattgat tttcttagtt tctggatata gtgagaacaa
     5221 cagtttttct tttctaatta tttgatttgc gaatgcaaac gatatacaat atgctcaatt
     5281 ttaattcata gaccaattaa attgtaccca ttcatcctta agcccaaatc cttgcgtttt
     5341 tttaaccttg tttttattgc tttaaccctg tctttctgtt tattagctaa tgcccaagcg
     5401 gcaggaattg aacgttatga cttcggggat atggcctcaa aggtgactaa agcttgggat
     5461 caagatgttg cgctttatgt tgccaagcgt ttcgcggtag aaaacagttt tgatgaaaaa
     5521 gagataacag taaaaaaatt aaatgcaggg gcgaccaacc atttcatata tttgatgttt
     5581 atcaataaca agttgaagtt tgttatcaaa ggtctgggaa cagaaaaaga ggcagaggca
     5641 ttgtttgctt tacaaaatta ctcaaaaatt cctgaaattc aaaataatcc acaagccgca
     5701 actgttacat taagtaaaat cattaattca tatagagtga agaatgattc gaggacttat
     5761 ttcttcgcaa ttttagaagc ggctcaagga aaagagttat ttaagataat gacttatgaa
     5821 ttgctacagc ataaagatat taacacgtta aacgatgttt tttatcgaat agggcaacag
     5881 acgggtgaat tacataaaac aattatcggg agtgatcaat ttatcactcc aagagattta
     5941 gtgaccgtta ttcatgatga ttttcatcct gaaaatacgt tttataatct tgaaacaaat
     6001 atcttctctt taattgataa tgaaagcatg ggtgatagta ttacagagcc cttgcccatt
     6061 atcagagaat tatatgggtt ttatgaagtt cctattattc gctggccctc agaaaaaata
     6121 actgttgatc tcttaaagga tgctgatcct catgatatag ccagtatcta ttacgacttt
     6181 gttctcggta tggcctcggt gtatcaagac ccccaagatg caaaacgagt gttaattgaa
     6241 gctattattg atttaaataa tatggctatt gcctttctga aggatagata ttctgagcca
     6301 tggatgctta acccaagcga taaacatgaa gcacatattg tgtggggcaa gttagcaaaa
     6361 gtcgacacta atccgcagct agcagaactc tatatcacta aattaaccat tattaataat
     6421 gtattgcaat cactttcagg ctttaaacgc tagcagaggg tgagtactct ggttattccg
     6481 caccctcgga ttttagctgc ttcagcagtc tttcattatt tgtttaaatg taataatggg
     6541 gctgaatttt aattactcta tatactgatg tagtgtgagc ttattttaga ttatttactt
     6601 t
//
LOCUS       DASLOE010000159         6569 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.160, whole genome shotgun sequence.
ACCESSION   DASLOE010000159 DASLOE010000000
VERSION     DASLOE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.160"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            89..715
                     /locus_tag="U6309_002439"
     CDS             89..715
                     /locus_tag="U6309_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1689 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505869.1"
                     /translation="MYHYLFSCHKSQESIDGLIEQVKQLLNHVEMEQKAYFLNLLTAR
                     VAEFQNELKSETSNTINKQQILIQYERFAKTLLICIKQPERTSSAIHNYHKGFYYPVA
                     IHDKIKPDPTIENAAIATLGVSLALLLGSIPTFIFNPLFGVIMVSLAVTLLLPSGFYL
                     LIPDSPDTTSKKEEEKRIFMEGAKIINPDVRIEEFDEQPYLSASLIKT"
     gene            800..3475
                     /gene="acnA"
                     /locus_tag="U6309_002440"
     CDS             800..3475
                     /gene="acnA"
                     /locus_tag="U6309_002440"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEN5505870.1"
                     /translation="MKVGQDSLSTKSQLTVDGKTYNYYSLKEAEKKHFKGINRLPYSL
                     KVLLENLLRFEDGNTVTTKDIKAIADWLHNKTSQHEIAFRPTRVLMQDFTGVPAVVDL
                     AAMRTAIVKMGGNADKISPLSPVDLVIDHSVMVDKFASADALEVNTKIEIERNQERYE
                     FLRWGQKAFSNFQVVPPGTGICHQVNLEYLGKTVWNSENNGQLYAYPDTLVGTDSHTT
                     MINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVIGFKLSGKLKEGITATDLVLTVTQM
                     LRKKGVVGKFVEFYGPGLNDLPLADRATISNMAPEYGATCGFFPVDKETIKYLELTGR
                     DKHTIALVEAYAKAQGMWYDKDNEEPVFTDSLHLDLGSVEPSLAGPKRPQDKVNLSSL
                     PVEFNNFLIEVGKEKEKEKTFAVKNKDFQMKHGHVVIAAITSCTNTSNPSVLMAAGLV
                     AKKAIEKGLQRKPWVKSSLAPGSKVVTDYLRHAGLQTYLDQLGFNLVGYGCTTCIGNS
                     GPLPDDISHCVAEHDLVVSSVLSGNRNFEGRVHPQVRANWLASPPLVVAYALCGTTCS
                     DLSREPIGQDKEGNDVYLKDIWPSNEEIAAEVAKVSGTMFRKEYAEVFKGDAHWQAIQ
                     TSSGQTYEWNPDSTYIQHPPFFENLSLKPEPLKPIKQAYVLALFGDSITTDHISPAGS
                     IKASSPAGLYLKSKGVDEKDFNSYGSRRGNHEVMMRGTFANIRIRNEMTPGQEGGVTR
                     YVPTGETMSIYDAAMRYQENQQDLVIIAGKEYGTGSSRDWAAKGTNLLGVKAVITESF
                     ERIHRSNLIGMGILPLQFKEGTTRKTLKLDGSERISIEISDKLTPGAMVPVTIERQDG
                     DIEKIETLCRIDTADELEYYKNGGILQYVLRKISS"
     gene            3740..5134
                     /locus_tag="U6309_002441"
     CDS             3740..5134
                     /locus_tag="U6309_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN5505871.1"
                     /translation="MNHSKKVLNVFSLVMINVIAVDSLRTLPISAKLGFSLVFYYIFA
                     ALTFFIPVALVAAELATAYPNTGGIYVWVREAFGRRAGFITIWLQWIYNVVWYPTMLA
                     FIAATLSYLIAPHLGNNKFYLLGTALTLFWVFTFLNCFGMKLSSIVSIIGASIGTLLP
                     MIVIIVLGAVWIFQDRPVAVNYPTTWLPDFSSLGNLSLFSAVLFGLIGMEMSAVHAEE
                     VKNPQRDYPKALFYSALLIISTLSLGSLAIVIVVPNDSLSVVSGLVDAYAIFFNSYNM
                     PWMTSVIAVLIILGGLSGVSAWIIGPTKGLLVSARDGSLPALFSRVNKYGSPVAILLT
                     QGVIFTVLSTVFILLDSINAAYWVLSDLSAQMALLVYIMMFAAAIKLRYSKPEQPRGY
                     TIPGGNLVMSLISGIGIICCIAAMIVGFIPPSQIPIKNVFLFECFLIGGLILFVFIPW
                     LFAKKHDEQLCSEE"
     gene            5428..>6569
                     /locus_tag="U6309_002442"
     CDS             5428..>6569
                     /locus_tag="U6309_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1692 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505872.1"
                     /translation="MARSKEEKALEPKDAENKFRVLTSDVAGNIPTHSSVTDVLNLIY
                     SSITTHNLFKPHLEPLKAKACVVQGDLATLILIAQRNPAALFQKGNITDPRERTFYNV
                     SAWQLILFLCDVDMKNQLMPFIPEQFKAIAQKQTNELGSGGADLIKLSRDPQAVAFED
                     FDGLTQFKQTLPLIDGTRKEVTFPLLENVDGIVYFQDKNKEVHFYYANRESKTITSLV
                     PDLKSEEDKQLFAAFKASFDAMENNSSRRSSDTEHQLIAKLFNAKPQRQGIQYEHQGI
                     RYQDSRTSFSLINAYRTSIRLYDEVQNYQYEKADTYWREVVGKAQGEEMWLLERICEE
                     GRPFHPLPADFKEFKRGFTFYNWITNKVESAFAGGKLAVGLGSDFA"
BASE COUNT         2013 a         1283 c         1298 g         1975 t
ORIGIN      
        1 atgcatgtaa aacctcctaa tactcactat aacccctaaa aaaataatgc tagttttttg
       61 acatatttta tagggagaga aatttatcat gtatcactat ttattcagct gccataaatc
      121 tcaggaatct atagatggct tgattgaaca agtaaaacaa ttactaaatc atgtggaaat
      181 ggagcaaaaa gcttattttt taaatttgtt gactgcccgt gttgccgaat ttcaaaacga
      241 acttaaatca gagacctcaa atactattaa taaacaacag atacttatcc agtatgaaag
      301 atttgcgaaa actttgctca tttgcataaa gcaacctgaa cgaacctcct ctgcaattca
      361 taattaccat aaaggatttt attatcctgt tgctattcac gataaaatta aacctgatcc
      421 cactatagaa aatgcagcta tagctactct tggagtcagc cttgctttgc ttttaggttc
      481 aataccaaca ttcattttta acccactctt tggtgttatc atggtatcac tggcagtgac
      541 attgctatta ccaagtggat tttatctact aatcccagat tctccagata caacaagcaa
      601 aaaagaagaa gaaaaaagga tttttatgga aggcgcaaaa atcattaatc ctgatgttag
      661 aatcgaagaa tttgatgaac aaccttacct ctccgcatca ttaatcaaaa catgatttta
      721 ttgatactga atgaaaactt cctgaaattg agctattatt cctagagatt attaataata
      781 atgattttgg gagtacttta tgaaagtggg tcaagacagt ctatcaacaa aatcacagtt
      841 aacagttgat gggaaaacct ataattacta tagtttgaaa gaagctgaaa agaaacattt
      901 taaaggaatt aatcgcctcc cttactcctt gaaagtactt ctcgagaatt tattacgttt
      961 tgaggatggc aatacagtaa cgaccaaaga catcaaggca attgccgact ggctccataa
     1021 taaaacatcc cagcatgaaa tagcatttag accaactaga gtcctgatgc aagatttcac
     1081 tggtgttccc gcagtagtag atttggctgc catgcgtact gccatagtaa aaatgggagg
     1141 taacgcagat aaaatttctc ctctttctcc tgtcgatttg gtcatagacc attccgtcat
     1201 ggtagataaa tttgcttcag cggatgccct tgaagtgaat accaaaatag agatagaaag
     1261 aaatcaggaa cgctatgagt ttttacgctg gggacaaaaa gctttttcca actttcaggt
     1321 cgtcccaccc ggaacaggga tttgtcatca ggtcaatctg gagtatttag ggaaaactgt
     1381 ttggaatagt gaaaataatg gccagttgta tgcctatccg gacactttgg tcggcacaga
     1441 ttctcacacg accatgatta atggactagg tgtcctgggt tggggagtcg gtggtataga
     1501 ggcagaagca gccatgctgg ggcaacctgt atccatgcta atccccgaag taatcggttt
     1561 taaattatca ggtaaactta aagaaggcat taccgcaact gatttggtcc ttaccgtcac
     1621 tcaaatgctg cgaaaaaaag gggttgttgg taaatttgtt gaattttatg gtcctggact
     1681 taatgattta cctttggcag acagagcgac aatttccaat atggctcccg agtatggcgc
     1741 aacatgtggc ttctttccgg tagacaagga aacaataaag tacttggaat taactggtcg
     1801 tgacaagcat accattgccc ttgttgaggc ttatgcaaaa gcacaaggta tgtggtatga
     1861 caaagacaat gaagaacctg tgtttactga ctctttacat ctggatttag gcagcgttga
     1921 accctctctg gctggcccaa aacgccccca agataaagtc aatctcagtt cattacctgt
     1981 agaattcaac aattttttaa ttgaagttgg taaagaaaaa gagaaagaaa aaacctttgc
     2041 tgttaaaaac aaagattttc aaatgaaaca tggtcatgta gtcattgcag cgataaccag
     2101 ctgtactaac acctctaatc caagtgtact aatggcagca ggactggtag caaaaaaagc
     2161 gattgagaaa ggattacagc gcaaaccctg ggttaagtct tcattggcac ctggttctaa
     2221 agtagttact gactatttaa ggcatgccgg cctacaaacc tacctggatc aactgggttt
     2281 taatttggtt ggttacggct gtaccacttg tataggtaat tcgggtcctt tgcctgatga
     2341 tatttcccat tgtgttgcag agcatgatct tgttgtttca tctgtactgt ctggtaatag
     2401 aaatttcgaa gggcgtgttc acccgcaagt cagagccaac tggctggctt ctcccccatt
     2461 agtagtcgct tatgctctat gcggtacaac ttgcagcgat ttaagcaggg aacccattgg
     2521 gcaagacaaa gaaggaaacg atgtttatct caaggatatc tggccatcta atgaagagat
     2581 tgctgcggaa gtcgctaaag taagcggtac catgttccgt aaagagtatg ctgaagtatt
     2641 taaaggagat gctcattggc aagccattca aacaagctca gggcaaactt atgaatggaa
     2701 tcctgattct acatacatac aacacccccc attttttgaa aatttaagtc tgaaaccaga
     2761 accgctcaaa cctatcaagc aggcttatgt tttagcccta ttcggcgatt caattacaac
     2821 ggatcatatt tcacctgcag gttcaataaa agccagctct ccagcaggct tatatttaaa
     2881 atccaaaggg gtagatgaga aagattttaa ctcctatgga tcacgccgcg gtaaccacga
     2941 ggtcatgatg cgaggcactt ttgccaatat acgcattcgt aatgaaatga ctcctggaca
     3001 agaaggtggc gtcactcgtt atgttcctac tggagaaacc atgtcgattt atgatgcagc
     3061 catgcgttat caggaaaatc aacaagactt ggttatcatt gctggtaaag aatatggaac
     3121 aggctcttct cgcgattggg cagcaaaagg cactaatttg cttggtgtta aagcagtgat
     3181 tactgaaagc tttgagcgaa tccatcgttc caatttaatt ggcatgggta tattgccatt
     3241 gcaatttaaa gagggtacta ctcgtaaaac attaaaattg gatggtagcg aacgcatcag
     3301 tattgaaatc tctgataaat taaccccggg cgcgatggta cctgtaacca tagaaaggca
     3361 agatggggac attgagaaga tagaaactct ttgtagaatt gatactgctg acgagctcga
     3421 atactataaa aatggcggaa tacttcagta tgtattgcgc aaaataagct cttaacaatt
     3481 gggcacattg cgtgcccttc tcccttagag tgttgggttc acaaaatatc tagagacgca
     3541 tgaacactgc atcactaggc agctgaaatc agaatatcag taaaaattga taccataatc
     3601 aggagaaacc caacaactta taaatattaa gcagtaatta aactgtacag acaaactttt
     3661 ctttactctg ttaacttatc ataataccat ttatacccca ttcaataccc gggtttattg
     3721 cactcataaa ggataaataa tgaatcattc aaaaaaggtt ctgaacgttt tttctttagt
     3781 tatgatcaac gtcattgctg tagatagttt gaggaccttg cctatcagcg caaaacttgg
     3841 attttctctg gtcttttatt atatttttgc agcccttacc ttttttattc cagtagcatt
     3901 ggttgctgca gagctcgcta ccgcctatcc taataccggc ggaatttatg tctgggtcag
     3961 ggaagcattc gggagacggg caggatttat cactatttgg ctgcaatgga tctataacgt
     4021 agtttggtat ccaactatgt tagcttttat cgccgccacg ttatcctatt taatcgctcc
     4081 tcatttgggg aataacaaat tttatttatt aggcacagca cttaccttgt tttgggtgtt
     4141 tacttttttg aattgctttg gcatgaaact atccagcatt gtaagtatta tcggcgcctc
     4201 cataggcaca ctacttccta tgatagtgat tattgtcctg ggcgcagttt ggatatttca
     4261 agatagacct gtagcagtca attaccccac aacatggtta cctgatttta gttctttagg
     4321 aaatctgtct ttattttcag cggttttatt tggccttata ggaatggaaa tgtctgctgt
     4381 tcatgctgaa gaagtaaaaa acccgcaaag agattatcca aaagcacttt tttattcggc
     4441 tcttttaatt atatccacct tatcattagg ttctttggca attgttattg tagtaccaaa
     4501 cgatagttta agtgttgtgt caggccttgt tgatgcctac gccatatttt ttaattccta
     4561 taatatgccg tggatgacat cagtgattgc cgtgttaatt attttaggag gcctcagtgg
     4621 cgtttcagcc tggataatag gtccaaccaa aggattacta gtgtctgcac gcgatggttc
     4681 cttacctgca ctgttttccc gtgtcaataa atacggctcg ccggtagcca tactgctcac
     4741 tcaaggcgtt atatttactg tattaagtac agtctttatt ttactcgatt caattaatgc
     4801 tgcgtactgg gtattaagcg atttaagtgc tcaaatggca ttgttggtat atatcatgat
     4861 gttcgctgca gcaattaaat tgcgatacag caagcccgaa cagccgcgtg gctatactat
     4921 tcctggtgga aatctggtaa tgtctcttat ctctggaatt gggatcatct gctgcatagc
     4981 tgctatgatt gtcggattta tccccccttc acaaatccct ataaagaatg tttttctctt
     5041 tgaatgtttt ctgatcggcg gccttattct atttgttttc attccctggt tatttgctaa
     5101 aaaacacgac gagcaattat gtagtgaaga ataattgagc aagaaatgta agcccacaaa
     5161 aaattttgtt gggcttttca agatgattct taaaagttgt taatcaattg taatataccc
     5221 aataaacttc aaactgcgag ttttacagcc tcttctttcg caatattctc acgcataata
     5281 tgctttttta aatcaccaag gctgcttagt attgttttct acctttcgat aattgccata
     5341 atatttgcca cattaaattg tttttatatg aacattaaat ttaatgtata atgctcaatc
     5401 atattgtgaa aataaccgaa tgtatgtgtg gcacgcagca aagaagagaa ggcattagaa
     5461 ccaaaagatg cagaaaataa gtttagagtc ttaacaagcg atgttgctgg aaatataccc
     5521 actcattcct ctgtaacgga cgtattaaat ttaatctatt cttccattac cacccataac
     5581 ttattcaaac cccatttaga accattaaaa gccaaggctt gtgtcgtgca aggcgattta
     5641 gctacattaa tattgatagc acaacgcaac cctgccgcgt tattccaaaa gggaaatatc
     5701 accgacccaa gagagcgaac tttttataat gtatctgcct ggcaacttat cctgtttctg
     5761 tgtgatgtgg acatgaagaa tcaactgatg cctttcattc cagagcagtt taaggccata
     5821 gctcaaaagc aaaccaacga gcttggcagt ggcggcgctg atttaattaa attatcccgt
     5881 gacccacaag ccgtggcttt tgaagatttc gatgggttaa ctcaatttaa acaaactctc
     5941 cccctcattg atgggacacg aaaggaagtc actttcccat tgcttgaaaa tgttgatggg
     6001 attgtttatt tccaagacaa gaataaagaa gttcatttct actatgcgaa tcgagagtcc
     6061 aaaacgatta catctctcgt accagacctc aagtcagaag aagataaaca attatttgct
     6121 gcatttaagg cctcctttga tgccatggaa aacaattcca gtcgtcgctc aagtgatact
     6181 gagcatcaat tgattgcaaa gctatttaat gccaaacctc agcgtcaagg cattcagtat
     6241 gagcaccaag gaatacgtta ccaggatagt cgcacttcat ttagtttgat taatgcctat
     6301 agaacaagta ttagactgta tgacgaagta caaaattatc aatatgaaaa agccgacacc
     6361 tattggcgcg aagtggtagg taaagcacaa ggtgaagaga tgtggctttt agagcgaatt
     6421 tgtgaagaag gccgtccatt ccacccttta cccgcagatt ttaaagagtt taaacgggga
     6481 tttacctttt ataattggat taccaacaag gtagaatctg ctttcgcggg ggggaaactt
     6541 gcagtcggtc taggttctga ttttgcgtt
//
LOCUS       DASLOE010000160         6515 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.161, whole genome shotgun sequence.
ACCESSION   DASLOE010000160 DASLOE010000000
VERSION     DASLOE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.161"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..216
                     /locus_tag="U6309_002443"
     CDS             <1..216
                     /locus_tag="U6309_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HEN5505873.1"
                     /translation="GFTLKEIKELLEFDAKNDRERVRDMAEKRIEKLNLKINELTEAR
                     NRLSLLVTKCIETSSSPCPILMTFEKS"
     gene            complement(253..414)
                     /locus_tag="U6309_002444"
     CDS             complement(253..414)
                     /locus_tag="U6309_002444"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505874.1"
                     /translation="MGTVFSLPVRFQPDELGRIQLLWYRHLRVLGVDRVWALGLPEKG
                     IAIIDELNN"
     gene            complement(522..1082)
                     /locus_tag="U6309_002445"
     CDS             complement(522..1082)
                     /locus_tag="U6309_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505875.1"
                     /translation="MPGTENHEFHTLTKSIVKNLLEFTGWNIAPGQKYIHSDPHQCYI
                     QHEGDQTLTIATLSSKVAKYKEEIRTGRTTSRSRDPVIDKCPEEYKLYRDFLDENCFV
                     RVRDSAKERLAAKFGVDSRGEVNAHFADLCELEMLGGQSVYEGFVNNYLVMSDKYIKA
                     HPQKDYDPDKINFLSSNPMGYTPKSS"
     gene            complement(1118..1837)
                     /locus_tag="U6309_002446"
     CDS             complement(1118..1837)
                     /locus_tag="U6309_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN5505876.1"
                     /translation="MIDFKINSQPDDESCGPTCLHAIYQYYGLDISYEEVANQVESFL
                     SGGTIAPMLGKHALQLGFKVTLYVNNLQLYDPTWFNSDGSSNTDVLREMLMQQMRYKR
                     NQYLARGTHAALDFLTLGGAIYFRCLTAEILKEYFARQIPILTGLSSTYLYNCSRELF
                     NNEGKSRSDPIRGLPCGHFVVLCGYNQKHRLVVVADPYKKNPVSGDNYYTVSIHRLIN
                     AIMLGVVTRDGNLLIIEPKYT"
     gene            complement(1982..2890)
                     /locus_tag="U6309_002447"
     CDS             complement(1982..2890)
                     /locus_tag="U6309_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN5505877.1"
                     /translation="MINFSQIQVFIAIVESGGFIAAGEKMHRSQPAITNAIKKLESSL
                     GFELFDRKVYRPQLTAKGKAFYQKAREVAAQFMQLENSAETLRSGVETQFSIDLDIAL
                     PLPGYAPLIQSFIRQYPETCFSLSNNTFVRCLERLERRECHLCIAASHIRSPQIEYLR
                     MPSIRMIPVASRDYYDQHRERINNPKLGIYCMQIMIAGTQQELYQLKIEPLSNSMPKW
                     LVGDMYIRKQLILSGMGIGRLPDHLVEKELAEGSLVQLNTRDYVAVTMDIYAMRLNNQ
                     EHGMVSNYAWQSLKSYADDTVSPDLK"
     gene            2996..3700
                     /locus_tag="U6309_002448"
     CDS             2996..3700
                     /locus_tag="U6309_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3865 domain-containing protein"
                     /protein_id="HEN5505878.1"
                     /translation="MIREYMDKRNNQFTTEILNYFSDLKKFSTSYLEWLTMEHYQFSL
                     NNTRFLMNSHIQTKSLSDPGVSEELLANYNEEKNHAQMYRHALKKIGLDVTNRIPYVA
                     TRQFFMRLEALIEINASKTLGVMYATETAAIFEHILFKEISAELCQRKRLDFENSRIK
                     QFHDLHLDGVEQAHKDELGKFMDHTLAQEHVDPNIFLQEKEIMDGAYAAIDIMEDWWS
                     GLINAKAHLLEQAHAQ"
     gene            3690..4424
                     /locus_tag="U6309_002449"
     CDS             3690..4424
                     /locus_tag="U6309_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN5505879.1"
                     /translation="MLNKLMWLTGKAQEDWIEKILHLRQKIWPDYQRIKNDTLFKLYS
                     HYPLYQWLSHYNGEVVAVINSLPVALDKSLDNLPQEGLNWVLSHLESTTPLTQSNYLA
                     AVSVTVNPAYQRLGLSKLLLNRLKASAQGAQYKALIVPARPSQKENYPLISLEDYLKW
                     KRVDNTIYDPWLRVHAQLGGKIAYPCQQSRVIKANIAQWEQWFGHPLLQTGQYLVKGG
                     LNPVTINKEKDIGIYIEPNVWVIHDL"
     gene            4733..5626
                     /gene="prpB"
                     /locus_tag="U6309_002450"
     CDS             4733..5626
                     /gene="prpB"
                     /locus_tag="U6309_002450"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEN5505880.1"
                     /translation="MSNFRYSQGKKLREALMKEKPLQVVGTINAYTALQAKRAGFHAI
                     YLSGAGVANASYGLPDLGITTLNDVLEDVRRIMSAVDLPLLVDIDTGWGGAFSIARTI
                     KEMIKAGAAAVHIEDQVQAKRCGHRPGKALVEKEEMIDRIKAAVDAKTDPDFVIMART
                     DALANEGLNKALERISAYIEAGADMIFFEGVRKLEEYQALTEQCNVPVLANITEFGVT
                     PLFTLEELKEVGVSLALYPLSAFRAMSAAAEKVYDTIRKNGSQKDILAEMQTREELYQ
                     VLNYHFYEDKLNELFMKEKTT"
     gene            5839..6321
                     /locus_tag="U6309_002451"
     CDS             5839..6321
                     /locus_tag="U6309_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEN5505881.1"
                     /translation="MWLDYLHKINSDPNLLYLFIRTFIIYVFAIFIIRVGNTRYNFET
                     AFDYIFVFVIGAVLSRAINGNSTLISAMAGSSLLIFLHWVFAICSFYSHGFGKLVKGK
                     SVLLIQDGQLIWSALKRHQVTEEDLRQVFREKLNASDLTTVKEARLERTGRISFIIYK
                     "
BASE COUNT         2100 a         1196 c         1243 g         1976 t
ORIGIN      
        1 ggctttactt taaaagaaat aaaagaacta ttggagtttg atgctaaaaa tgatcgagag
       61 cgcgtaagag atatggctga aaagcgtatt gaaaaactta atttaaaaat taatgaatta
      121 accgaagctc ggaatagact ttcactctta gtaacaaaat gtatagaaac cagctcttcg
      181 ccttgcccca ttcttatgac ctttgagaaa agttaaaccc ataaaaatag aaagcaatca
      241 aattattgat aactagttat taagctcatc aatgattgca attccctttt ctggcaagcc
      301 aagcgcccaa actcgatcga cacctaaaac acgtaaatgc ctataccaaa gaagctgtat
      361 ccgtcctaat tcatcgggtt gaaaccgaac tggaagagaa aatactgttc ccaaattgtg
      421 ggatgtttgg gtgatattat cctttaaccg gattttagca atgagcttac cttatcgaca
      481 taacaatagc agggcatggg aagagggtta cttgctttga attaacttga ttttggggta
      541 tagcccatag gattagaaga aagaaaatta attttatcag gatcataatc cttttgaggg
      601 tgtgctttta tgtatttatc tgacatgact aaatagttat taacaaatcc ttcatagacc
      661 gattgccctc ccagcatttc caattcacaa agatcggcaa aatgggcatt cacctcaccg
      721 cgactgtcca caccaaattt agcagctaac cgttcttttg cagaatctct aactctgaca
      781 aaacaattct catcaagaaa gtcacgatat agtttgtatt cttcagggca tttatcaata
      841 acaggatctc ttgagcggga tgtggttctc cctgttctta tttcctcttt gtatttagcg
      901 accttggagg ataaagtcgc gatagttaag gtttgatccc cttcatgctg aatataacac
      961 tggtggggat ctgaatggat atacttttgc ccaggggcaa tgttccatcc ggtaaattcc
     1021 agcaaatttt taacaatgga ttttgttaat gtatgaaact catggttttc agtaccaggc
     1081 ataggtttgt agtaagcgtt taaaaataaa aactcatcta cgtgtatttc ggctcaataa
     1141 tcaataaatt accatcacgt gtcactactc ccaacataat tgcgttaatt aatctatgga
     1201 tggatacggt gtaataatta tcacctgaaa ccggattctt tttgtaagga tcagcgacca
     1261 ctactaagcg atgtttttgg ttgtatccgc atagaacaac aaaatgtccg cacggaaggc
     1321 cgcgaatagg atcactcctc gatttgccct cattgttgaa taattcgcga ctgcagttat
     1381 acagataggt tgaactaagc cctgtcaata tgggaatctg cctggcaaaa tattccttta
     1441 aaatttctgc agtcaggcat cgaaaataga tagcgccccc caaagtcaga aaatcaagtg
     1501 ctgcatgtgt tccgcgggca aggtactggt tacgcttata cctcatttgt tgcatgagca
     1561 tttctctcag aacgtcggta tttgatgaac catcgctatt aaaccatgtc gggtcataca
     1621 attgtaaatt attgacataa agggtcactt taaatcccaa ttgcaaggca tgtttaccaa
     1681 gcatgggcgc tattgttcca ccagataaaa agctctctac ctggtttgcc acctcttcat
     1741 aactgatatc caagccataa tattgatata tggcatgtaa acaggttggc ccgcaagatt
     1801 catcatcagg ttgggaattt attttaaaat caatcattat aatatccaat tggagatcaa
     1861 ttttatgtct ttttgattta aatttaggct tatcttgatt ttatgtcaat gaatcacctc
     1921 ctgtttttca aatattcggt ccaccaaact gcttgaatta acaagcaaac ataaagattg
     1981 attatttcaa atcgggagat actgtgtcat cagcataact ttttagtgat tgccaagcat
     2041 aattggagac cataccatgc tcttgattat ttaaccgcat agcataaata tccatagtaa
     2101 cggcaacata atccctggta tttaactgaa ccaaagagcc ctctgccaac tccttttcaa
     2161 ccagatgatc cggtaaacgg ccaataccca tacccgataa aatcagttgt ttacgaatat
     2221 acatatcacc cactaaccat ttgggcatag aattacttaa tggctcaatc ttgagttgat
     2281 ataattcctg ttgcgtgcca gctatcataa tttgcatgca gtatattccc aacttgggat
     2341 tatttattcg ttctctatgc tggtcataat agtccctgct cgcaacagga atcattcgta
     2401 tggaaggcat acgcaaatat tctatctgtg gagaacgaat atggcttgca gctatacata
     2461 aatggcactc tcttctttcc aggcgctcaa gacatcgaac aaaagtattg ttacttagtg
     2521 aaaaacaagt ttcgggatat tgtcttatga aagattgaat aagcggagca tatcctggca
     2581 atggtaaagc aatatcgaga tctatagaaa attgtgtttc tactccggat ctcaatgttt
     2641 cagcactatt ttccagttgc ataaattgag cggccacttc tcttgctttt tgataaaaag
     2701 ctttaccttt cgcagtgagt tgaggtcggt atacttttct atcaaacagt tcgaagccca
     2761 ggctggactc caattttttt atggcattag ttatagccgg ctgtgaacga tgcatcttct
     2821 ctccagcagc aataaatcca ccagattcaa caattgcaat aaacacctgg atttgtgaaa
     2881 aatttatcat aactaaaaat tatcaagata attataaaat aatattttaa ttatatgatc
     2941 tttatagtta tggtaaaccc ttgatgaatt gattttaata ttgacgggag gcttcatgat
     3001 aagagaatac atggataaga gaaacaacca atttactacc gaaattttaa attattttag
     3061 tgacctaaaa aaattttcga cgagttatct tgaatggcta accatggagc attatcaatt
     3121 ttcgttaaac aatactcgtt tcttaatgaa ttctcatatc caaaccaagt cactttcaga
     3181 tccaggagtt tctgaagagt tactagctaa ctataacgaa gaaaaaaatc atgctcaaat
     3241 gtatcggcat gcactaaaaa aaatcggtct ggatgtaaca aacaggattc cgtatgtggc
     3301 aaccagacag ttttttatgc gccttgaagc cttgattgag attaacgcat ccaaaaccct
     3361 tggcgttatg tacgctactg aaacagctgc aatatttgag catattttat ttaaagaaat
     3421 aagcgccgag ctttgtcaaa gaaaacgttt ggattttgag aattctcgca tcaagcaatt
     3481 ccatgattta cacctagatg gagtagaaca ggcacataaa gatgagttag gcaagtttat
     3541 ggatcatacg cttgctcaag agcacgttga tcccaatatt tttctccaag aaaaagaaat
     3601 aatggatggc gcatatgcgg cgatcgatat aatggaagat tggtggtccg gtttaattaa
     3661 tgcaaaagca caccttttgg aacaagctca tgctcaataa acttatgtgg ctaactggca
     3721 aggcgcaaga agactggata gagaaaattc tacatttacg acaaaagatt tggcctgatt
     3781 atcaacgcat taaaaatgac actcttttta agctctatag tcattatcct ttatatcaat
     3841 ggctatctca ctataatggt gaggtagtag ctgttattaa ttctttgcca gtagcacttg
     3901 ataaatcact cgataatttg ccacaagaag gattaaattg ggtcctgtct catctggaat
     3961 ctacaacacc cttaacccaa tcaaattatc ttgctgctgt atcagtgacc gtgaatccgg
     4021 cttaccaaag gcttggttta agcaaactct tattgaatcg gttaaaagcc agtgcacagg
     4081 gggctcaata taaagcacta atagtgccag ccagacctag ccaaaaagaa aactacccgc
     4141 ttatttccct agaggattat ttgaagtgga aaagagtgga taatactatt tatgatcctt
     4201 ggttaagagt tcatgcacaa ctgggaggaa aaatagctta tccatgccag caatcacgcg
     4261 tgatcaaggc gaatattgct cagtgggaac aatggttcgg ccatccgtta cttcaaacgg
     4321 gtcaatatct tgttaaaggc gggttaaatc ctgtcactat caataaagaa aaggatattg
     4381 gaatatatat tgagccaaat gtttgggtta ttcatgattt gtagttgatt tttgccatct
     4441 ctcgttataa taccaggcaa acaagcttgt ctttggtaca tgctctatac gcttttcaag
     4501 ctttggatgt cgttttgctg tgcatttcca gaagtttgaa aaaatatcta aaagaacgta
     4561 aaaatcaaat aaaccagact tacgaaaaac cccaagatca aagcaaaaaa tgtttttaat
     4621 gagggagttt agataaacta aatgaccgaa ttaaaaactt tttttaacaa agagattggg
     4681 atttttagta agtcctgata aattctcatc ttaggcaaaa caaggaaacc aaatgtcaaa
     4741 ttttcgatac agtcagggaa aaaaactacg cgaagcttta atgaaagaaa aaccattgca
     4801 agttgtagga acaattaatg cctataccgc tttgcaagcc aaaagagcag ggtttcatgc
     4861 aatttatctt tcaggagcag gagtagctaa tgcctcttac ggcttaccag acctgggcat
     4921 taccacatta aatgatgtac tcgaagatgt gcggcgcatt atgagtgccg ttgatttgcc
     4981 cctcctggtc gatattgata caggatgggg tggagccttt agcatcgcac ggactatcaa
     5041 agaaatgatt aaagctgggg cagcagcggt acatattgaa gatcaagttc aagcgaaacg
     5101 atgtggccat cgcccgggta aggcattggt tgaaaaagaa gaaatgatag atcgtattaa
     5161 ggccgccgtg gatgccaaaa ccgatcctga ttttgttatt atggcaagaa ccgatgccct
     5221 agcgaatgaa ggtttaaata aggcgctaga gcgaattagt gcttatattg aggccggggc
     5281 agatatgatt ttcttcgaag gagtacgtaa acttgaggaa tatcaggcgc taactgagca
     5341 atgtaatgtt ccggtattgg cgaatatcac tgaatttgga gtcactccat tatttacgct
     5401 ggaagaatta aaagaagtag gagttagcct ggctctttat cctttaagtg catttcgggc
     5461 aatgagtgcc gctgcagaaa aagtttacga cacaatccgc aaaaacggca gccaaaaaga
     5521 tattctggca gagatgcaaa ctcgcgaaga gttgtatcag gtattaaatt atcattttta
     5581 cgaagacaag cttaatgaac tctttatgaa agaaaaaacg acgtgaatat caattaatta
     5641 tcagaactaa tttgataaaa taaggtttta tatgcctgga tagaggatta tctgccgaaa
     5701 ttgatgttta taaacaacag attaactctc tatccagaca agcattcaaa agcaatccat
     5761 ttaaaatttg ataaagttat tgcaaaagaa aagcaattct atttatagtt ttgggataat
     5821 agatttgttg aaacagatat gtggcttgac tacctccata agattaattc cgatccaaac
     5881 cttttatatc tttttataag aacttttatt atctatgttt tcgcaatttt tatcatccgt
     5941 gttggcaata cccgatacaa ttttgaaaca gcatttgatt atatttttgt atttgtcatt
     6001 ggcgccgtgt taagtcgtgc aattaatggc aactctactt taatatctgc aatggctggg
     6061 agcagtttac ttattttttt acattgggtc tttgctattt gctcctttta ttcccacgga
     6121 tttggaaaac tggttaaagg gaaatcggtg ctattaatac aagatgggca gctcatttgg
     6181 agtgctttga aaagacatca agtgactgaa gaagatttga ggcaagtttt cagagaaaaa
     6241 ttaaatgcaa gcgatttgac aacagtgaaa gaagctcgtt tggagcgaac ggggcgtatc
     6301 agttttatta tctacaaata attttgtaac aagagcgaat tgtggcgatt acttttttcg
     6361 taaatcctgg ctgtttcatt ggatagaaat tgtatcttga aaattaagaa tactcttcta
     6421 ttctttatgt aaagaattct tttaacgtaa ttatttgatt cttatatggc tcgtttaaag
     6481 acctgcgtgc ctccgcttat ccaagacatg cagcg
//
LOCUS       DASLOE010000161         6308 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.162, whole genome shotgun sequence.
ACCESSION   DASLOE010000161 DASLOE010000000
VERSION     DASLOE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.162"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..927)
                     /locus_tag="U6309_002452"
     CDS             complement(<1..927)
                     /locus_tag="U6309_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505882.1"
                     /translation="MLVNWLRITKTVLMFLIIFSGEVFAESTKPVMVFNYPVEAIYLI
                     NNPKDGDISWGHDYEEACKKMGDRFFWGIVPSKGVDPACYSLPTYNMIIGADTWFCAN
                     GSISYFNKPHNFCNDFTSCPDQSWTLATDKSICTRENRPCIPDPDSVSEEQLLAAVAY
                     GESHWSNDYEEMAGIASATLRRMKAYGYNSVSELISKDPNFAYATALKTKNERYYNLM
                     CNINSNGVELAYKAARNALSKGADYSNGGCFWDGVDVKTNGASAYRYKYGFKFSNPKH
                     NVLDVTEPKPFHKKGRNGKYFSHTYVSTAGHNK"
     gene            complement(1223..2158)
                     /locus_tag="U6309_002453"
     CDS             complement(1223..2158)
                     /locus_tag="U6309_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN5505883.1"
                     /translation="MAFLQLVAQTNADMVIHAGDFGFYDEQSIYRLSPRELRLLVSHS
                     PVWRQYEVDKQTNRERLIEIVKENRLLGDFHDFISGEKKFSVPVYAVWGNHEDVQVLR
                     QLNANLRIENLHVLDEHHFYQFHTDENELEFSLYGLGGNFLVSKKLFDKPIAGNGGKV
                     WSVLHQFGSLYQQVKNKSKPSIFVSHVSPGKEPLLSRLIIHFMPNFWISGHMGAPFTR
                     TWNQFTIREMNESLDWLESDINLIEEQYQQGHLTDEARLACEIIKRPIDRDEFWYKKL
                     WNINLPDVADGHALLILKDGKFSLETYSNGIKFNN"
     gene            complement(2259..3110)
                     /locus_tag="U6309_002454"
                     /pseudo
     CDS             complement(2259..3110)
                     /locus_tag="U6309_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634942.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(3125..3793)
                     /locus_tag="U6309_002455"
     CDS             complement(3125..3793)
                     /locus_tag="U6309_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505884.1"
                     /translation="MLEFVARKLGELNNQVVYLGGCTTALFINDPLSLDVRPTLDVDC
                     IIDVLSLGEYYKFEEKLKEKGFHRSMQDDVTCRWHYDDIILDVMPTDENILGWGNFWY
                     KEAIKHAVTHQIAEDISIKSVTAPYFLATKIEAFKSRGNNDFLGSHDFEDIITVISGC
                     IDISEQVQASSDDLKSHLKLIFNEFLKNDQFTQSLPGHVSDGLVTMQRVQTVMERIEK
                     LAKE"
     gene            complement(3796..4368)
                     /locus_tag="U6309_002456"
     CDS             complement(3796..4368)
                     /locus_tag="U6309_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505885.1"
                     /translation="MLKPQDIVILLKILASEHPEQLLQKDLAAYLCMSASEIHEGIKR
                     LELSGLLGPVYRKSEDSNKIIRMPIKSACEECLVYGVKYFFPLKLGSYTRGIPTSYAA
                     PLFEKHLMLGDDPIPVWPYAEGERRGLALEPLYRSVPQAIAKHPDQPFYELLVLIDAI
                     RTGRARERNIAIKLLREKLCLKETNEQAQI"
     gene            complement(5096..6001)
                     /locus_tag="U6309_002457"
     CDS             complement(5096..6001)
                     /locus_tag="U6309_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN5505886.1"
                     /translation="MINNSLNLNDLAIFALVVKKRSFTQAALEAGISKAWVSQKISQL
                     EGKLDIKLLNRTTRSLSLTLGGEILFEHCQAMLKEVIHAENHLREYAKAPSGKLVITC
                     PEISGLDLFPTLLHQFKLLYPQIKIRLIITDQFVDLTQQGIDFAFRTGKLTDSGLVSR
                     YIGAVPRCLVASSEYLSNNSPIKNPADLFNHQLLKHSSLPDWFLKKEEEVFKIHVKNP
                     VIESNSLIFLHKMSCLHHGIAFLPYYLCSDALKHKMLVKVLPDWLNVDNEYYMVYHKE
                     KSVLYIHQLFKDFILQSDLNKMISN"
     gene            6092..>6308
                     /locus_tag="U6309_002458"
     CDS             6092..>6308
                     /locus_tag="U6309_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HEN5505887.1"
                     /translation="MRFIIIFIYSLLLNPVFAEVIHYQTDTFAKIAAQYKKLGVDPKT
                     TLMVFDLDDTLITMTQPLGSVGWWDWQH"
BASE COUNT         1959 a         1256 c         1044 g         2049 t
ORIGIN      
        1 cttattatgt cccgcagtcg atacataagt atggctaaaa tacttaccat tcctcccttt
       61 tttatggaat ggtttaggct cagtaacatc aagtacattg tgttttgggt ttgaaaattt
      121 aaatccgtat ttatatctat aggcagatgc tccatttgtt tttacatcta ccccatccca
      181 aaaacatcca ccatttgaat agtctgcccc tttcgacaaa gcgttcctag cagctttata
      241 agctaattct actccattgc tgttaatatt acacatcaaa ttatagtacc gttcattttt
      301 tgtcttaagg gctgtggcat aagcaaagtt gggatctttt gaaatgagtt cactaactga
      361 attatatcca tatgctttca ttcttcgcaa agtagcactc gcaattcctg ccatttcttc
      421 ataatcattg ctccaatgtg attcgccata agcaacagca gctaataatt gttcttcaga
      481 aacactatca gggtcaggaa tacatggtct attttcgcga gtacaaatgg atttatctgt
      541 tgctaatgtc caactttgat ctgggcaaga agtaaaatca ttgcaaaaat tatgaggctt
      601 attgaaatag ctaattgacc catttgcaca gaaccatgta tctgctccaa ttatcatgtt
      661 atatgttggt aacgaatagc aggcaggatc gacacctttt gaaggaacaa taccccaaaa
      721 aaatctatct cccatttttt tgcaggcttc ctcgtaatca tggccccaag aaatatctcc
      781 atctttaggg ttattgatca ggtaaatggc ttcaacagga taattaaaaa ccattactgg
      841 cttagttgat tcagcaaata cctcgccaga gaagataatt aaaaacatca aaactgtttt
      901 tgtaattctc aaccaattta ctaacattaa atgccattaa aacaaaagtg cttatatttt
      961 aacataatat ttttctttag atgcactctg tttataaaat gctgataatt ctgcacattg
     1021 aaacatagcc ctcattaaga gcaaaattcc aatattttaa tttattcgaa ttctcatgtt
     1081 gaattttctt tacataagta aaattcaagt atacataaat actatataat tataggattt
     1141 ccgtatactt aattagatga gctttatatt taaggaatgt tttgttccca gatggtttcc
     1201 tacatgtctg caatagcagt attcaattat taaatttgat tccgtttgaa taggtctcta
     1261 aggaaaattt accatctttc aaaattagaa gtgcatgtcc atcagccaca tcaggaagat
     1321 taatattcca caatttttta taccagaatt catccctatc gataggcctt tttattatct
     1381 cacaagctaa tctggcttca tctgtaaggt gaccttgctg atattgttcc tctattaaat
     1441 taatatcgct ctctagccaa tctaaggatt cattcatttc ccggatagta aattgattcc
     1501 aggtacgggt aaacggtgca cccatatgac cgctgatcca aaagttaggc ataaaatgga
     1561 tgattaagcg agataaaagt ggctctttgc ccgggctgac atgggataca aatatcgatg
     1621 gtttactttt atttttaacc tgctgataca aggaaccaaa ttgatgcaat acagaccaaa
     1681 ctttgcctcc attaccagca attggtttat cgaataactt cttgcttacc aaaaaattac
     1741 cacccaaacc ataaagagaa aactctagtt cattttcatc cgtgtggaat tgataaaagt
     1801 gatgttcgtc aagcacgtgc aggttttcaa tacggagatt ggcatttaat tgcctcaaaa
     1861 cttgcacatc ctcatgatta ccccacacag catatacagg aactgagaat ttcttttccc
     1921 ctgagataaa atcgtggaag tctcctagca accgattttc tttgactatt tctattaacc
     1981 tttcacgatt agtttgttta tcaacttcat attgtcgcca aactggtgag tgggatacta
     2041 gcaagcgtaa ttcccttggg cttagtcgat aaatgctttg ttcatcataa aacccaaagt
     2101 ctcctgcatg aatcaccata tcagcgttag tttgagctac cagttgaagg aaagccaccc
     2161 tcggctatcg tggctgaatt aagcgaaata actcccttcg gtgaagaagt gagtaataac
     2221 tataaaatag acaatgaacg catccaaata gccaattatc atacagattt gatcatgaaa
     2281 aaattgaaag gatctctatg aatcacacaa atgattgtct cttgtataac gggcttataa
     2341 tattccgctt taccagcttt cacaattaca ttggtccgtg gctgatattc ggcatcacta
     2401 tgcgagtgcc cacaaagcac taaaaaatcg attgaaggat tctcaattgc aaaaggcatc
     2461 aatatatcgc ctatcgcttt agagctgaaa taaggcggaa aattatcatc actcacttgc
     2521 gccatatgct gagaagcctc cttgaatgga gggacgtggg ttaacactgt gatcttttgg
     2581 ggttgttgct taatggcttg aattagatca tttttcaacg cactagcatc caaatcagcc
     2641 aaagcctaca ttttttgtaa cagatgctgt caacttagca ttttttcctg aaacaaatca
     2701 gcaatcattc ggctgtcatt gagcgaaaca gggctatttt gataatcacc caatcgacca
     2761 tccgcccaac catcttaccc taataaaatt gtattattga gaagttgttg caaaccagtc
     2821 gctggtaacc aaaataaatt ggcatttgcc atacttaatg cagtcagttc cttacgaacc
     2881 tcgttaatgc ttcctctata ataatcatgg tttccaacaa taaaatatat gggcttttta
     2941 atttgcccag ccatttcgtt tagaatatca attaaggaag gcacttcggc aatatcccca
     3001 cttattaata atacatcaca accgttggcc agaatgctct cataatattt ttggcgcgat
     3061 tcaactttta gtaaattgag gtgaatatct gttagccagg cgagtttcat ttttatatac
     3121 cctattattc ttttgcaagt ttttctattc tttccatgac agtttgaacg cgttgcattg
     3181 tcactagccc gtcactaaca tggcctggaa gtgattgtgt gaattgatca ttttttaaaa
     3241 actcattgaa tattaatttt aaatgagact ttaaatcatc acttgacgcc tgcacttgct
     3301 cgctaatatc aatacaccct gatatcaccg taataatatc ttcaaaatca tggctgccaa
     3361 gaaaatcatt gttccctctt gatttaaatg cttcaatttt tgtggcaaga aaataagggg
     3421 ctgttacaga cttgatcgag atatcctctg caatctgatg agtaaccgca tgtttaattg
     3481 cctccttata ccaaaaattt ccccaaccca agatattttc atcagtaggc attacatcaa
     3541 gtatgatgtc gtcataatgc caacgacagg taacatcatc ttgcatcgag cgatgaaaac
     3601 ctttttcttt cagtttttct tcaaacttgt aatactcccc taatgataag acatcaataa
     3661 tgcagtccac atccaatgtt ggccgaacat ctagtgaaag cggatcattg ataaaaagtg
     3721 cggtggtgca accacctaga taaaccactt gattgtttaa ttcaccaagc ttcctggcaa
     3781 caaattccaa cattcttaaa tttgagcttg ctcgttggtt tctttgagac ataacttctc
     3841 tcttaatagt ttaattgcga tattccgctc tcttgctctt cctgtacgta tagcgtcaat
     3901 cagaaccagt aattcataaa atggttgatc ggggtgtttg gctatggctt gaggtactga
     3961 tcgatacaaa ggctctaagg ctagtcctcg acgctctcct tctgcatatg gccataccgg
     4021 tatgggatca tcacctaaca tgagatgctt ttcaaataaa ggggctgcat aacttgtggg
     4081 aatacctcgt gtataggatc ccaatttcaa agggaagaag tattttactc catatactaa
     4141 gcactcttcg cacgcagatt ttataggcat tcggattatt ttatttgaat cctctgactt
     4201 acgataaaca ggacctaata atcctgataa ttctaaacgc ttaatccctt catgaatttc
     4261 agaagcactc atgcaaagat aagcagccaa atctttttga agcaactgtt ctggatgctc
     4321 actagctaat atttttaaaa gtatcacgat gtcttgtggc tttagcattt gtcattacca
     4381 gttcatcact atactcaaat catagcataa attcgcaatt tgcgaatcgc gaattacgaa
     4441 ttaacaatct catcagatta tttaagatcg agaatgatct gtgtccccca agtcccaatg
     4501 ttacaaaatg ggatgaggac ggggctagcc caatgatagc gccaatgccc cttttgtccc
     4561 cgagaatttt tgcatgacta tctatggtat taatcatcat gagtttcatc atacgtagaa
     4621 atcatgttac tgataatgtc tataacccta tcaatttatg agattcatca tcccagacga
     4681 tatccaataa ctgctgaaaa acactagcca gctgattata gtcctccaca ttttgaggct
     4741 cacgtattat tgtttccagt gctctattat ttcattgata ttcatacttc agccgtctcc
     4801 atcatatgct caagtataaa attaacaacc tgctgcattg gtttacgcaa ccgatcatca
     4861 tccttcatga tatatgctac tgtagcatca ttgttcattt tatgattaag aagtcgcttt
     4921 aacgcataag caggtaaatc aagactatct gcgtaggttc ataaatataa ccggttttac
     4981 tttcagctgg gaatacaaat tcactggttt tattctggct tctgcgctca attagctcat
     5041 agacaaaatc agatataggc aagatgtatt tcttgatttt tgggtctatt cgcgcctaat
     5101 ttgaaatcat tttgtttaaa tcagattgca atataaaatc tttaaacaat tgatgtatat
     5161 aaagaacgct tttttctttg tgatatacca tataatactc attatccaca ttaagccaat
     5221 caggtaatac cttaaccaac attttgtgtt tcaaagcatc tgaacataaa tagtaaggca
     5281 gaaaagcaat accatgatga aggcatgaca ttttatgcaa aaaaattaag gaattacttt
     5341 ctattacagg atttttaaca tgtattttaa atacctcttc ctcctttttt aaaaaccaat
     5401 caggcaagga gctatgtttt agcagctgat gattaaacaa atctgctgga tttttgattg
     5461 gtgaattatt agataaatat tcagaagagg cgactaagca tcgtggtact gctccaatat
     5521 agcgggatac taatcccgag tcagtcaatt tcccggttct aaaggcaaaa tcaatgccct
     5581 gctgggttaa atccacaaat tgatcggtaa taattaaacg aatcttgatt tgaggataaa
     5641 gtaatttaaa ctgatgcaat aaggtaggaa ataagtctaa ccctgaaatc tcaggacagg
     5701 taataaccag ttttccagaa ggtgccttag cgtattcccg taaatgattt tcagcatgaa
     5761 tcacttcttt caacatggct tggcaatgtt caaataagat ttcaccacct agagtaagac
     5821 ttaaagaacg cgtggttctg ttaagtaatt ttatatcaag cttaccctcc agctgagaga
     5881 ttttttgact gacccatgct ttggaaatcc cagcctccaa tgcggcttga gtaaagcttc
     5941 gcttttttac tactaatgca aaaattgcca aatcatttag attaagtgaa ttgtttatca
     6001 tagcgaacaa tgagtttata ttatttggtc taatcaatta attggctaat ctatatcatt
     6061 aacgcttgat atacaataac caggagttat catgcgtttt ataataattt tcatttattc
     6121 attgttactt aaccctgttt ttgctgaagt aattcattat cagaccgata cttttgcaaa
     6181 gattgcggca cagtataaga agttaggagt ggatccgaag accactttaa tggtgtttga
     6241 tttggatgac acattaatta ccatgacaca acccttaggc agtgttggtt ggtgggactg
     6301 gcaacatg
//
LOCUS       DASLOE010000162         6262 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.163, whole genome shotgun sequence.
ACCESSION   DASLOE010000162 DASLOE010000000
VERSION     DASLOE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.163"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            16..570
                     /locus_tag="U6309_002459"
     CDS             16..570
                     /locus_tag="U6309_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEN5505888.1"
                     /translation="MNLIKIKSKTLFMVLVYSLLSPAYAASHPKSTVNDTAIQNEILV
                     YINNYRQQHGLAPLKMDNRIVKEAKIHSMDMAKHAIPFGHKYFKKRIDKLHTQIKNSN
                     AGAENVAYNYKNAQDVVKNWLRSPGHKRNIVGNYDLTGVGIARDEKGKIYFTQIFLKT
                     GNSHYASRKPFPSIFSGALFSKKA"
     gene            964..2097
                     /locus_tag="U6309_002460"
     CDS             964..2097
                     /locus_tag="U6309_002460"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEN5505889.1"
                     /translation="MMIATLMESAQETRVAITPNSAKQYIKLGYDVGIEKNAGLSSDF
                     ANEEFEKAGAIIFDSKSTLLKKTQILLCVNEPDPKNLNGLPPDSLIIGHIDNNPLSPL
                     VKWCLDQKITLFSMSLIPRISRAQSMDSLSSQANLAGYRAVLEAATTFHRAIPMMMTA
                     AGMIQPAKVLILGAGVAGLQAIATAKRLGAVVYAFDVRKAAKEQVESLGAEFIEVMQE
                     QDSETAGGYATETSEEYKKLQAELIDQYAKTADIVISTALIPGRQAPVLLYKKTVEQM
                     KPGSVIVDLATSRGGNCELSVADKIIKHGDITIVGFSNMAGLVPATASDLYSNNLVHL
                     INLLAKNPAEFNLNPEDEIIQQAVLCHKGTYLPFQGVKEKQNA"
     gene            2090..2386
                     /locus_tag="U6309_002461"
     CDS             2090..2386
                     /locus_tag="U6309_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEN5505890.1"
                     /translation="MPEIHTLDNPYITILTIFVLACFVGYYVVWKVTPALHTPLMSVT
                     NAISSIIILGALIAAGSELIGSITWLGGIAIFITSINIFGGFVVTQRMLRMYKK"
     gene            2410..3807
                     /locus_tag="U6309_002462"
     CDS             2410..3807
                     /locus_tag="U6309_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEN5505891.1"
                     /translation="MNSIVPLFYLLAAICFILALKGLASPATSRRGNLLGILGMILAV
                     GSTLMMPTTYHHILLIALIAGGGIIGTIIALKINMTAIPQLVAAFHSLVGMAAVLVAY
                     CAFLSPASFQIGTPGAIATASLIEMSLGLIIGAITFSGSIIAFLKLQGLISGIPIRFY
                     AQNYVNLLIGLTILVLLSLFVANQNLFLFNLLAGLAFLIGVLLIIPIGGADMPVVISM
                     LNSYSGWAAAGIGFTLSNHLLVITGALVGASGAILSYIMCVGMNRSIFNVIFGGINSS
                     GNTKQAHSANELRNVRQANGEDAAFLLSNAKDVIIVPGYGMAVAHAQHAVKELVDALE
                     SRSVRVRFAIHPVAGRMPGHMNVLLAEANIPYDRVFEQSEINRDFATCDVAYVIGAND
                     ITNPAAKTDPASPIYGMPVLEVEKAKTVLFVKRSLSPGYAGVENDLFFHDNTYMLFGD
                     AKVMTESIAKALGEI"
     gene            3942..4325
                     /locus_tag="U6309_002463"
     CDS             3942..4325
                     /locus_tag="U6309_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505892.1"
                     /translation="MLRKIGFTLLCAVATFSSSTYSSVTHTLQAGVTLEYEFPPNEPL
                     VFVNYLYWEVAADCKITSEDPSNELFAEALAKAGKINGVTLSKGNTMKVTVHSGDILK
                     LSAESGAKVQITNFGPHTLKATCTA"
     gene            complement(4391..5971)
                     /locus_tag="U6309_002464"
     CDS             complement(4391..5971)
                     /locus_tag="U6309_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEN5505893.1"
                     /translation="MSDFYQDFNKRRTFAIISHPDAGKTTVTEKLLLFGGAIQLAGTV
                     KGRKADRHATSDWMEMEKERGISITTSVMQFIHNQHVINLLDTPGHEDFSEDTYRTLT
                     AVDSALMVIDVAKGVEERTVKLMEVCRLRDTPIMTFINKLDREGREPIDLLDEVESVL
                     GIQCAPITWPVGMGKRFKGIYHRYQDIIYLYQQGSNAKKVEAMQIKGLDNPQLDELIG
                     DSADELREEIELVKGASHGFNLEDYLAGKMTPVYFGSAINNFGIKELLDDFVEYAPGP
                     QPRATQERVVSPNEETFSGFVFKIQANMDPKHRDRIAFLRVCSGAYKKGMKLSHLRIG
                     KEVQISNALTFMAGDRSHTELALAGDIIGLHNHGTIRIGDTFTQGEQLKFTGIPNFAP
                     ELFRLVRLRDPLKSKALLKGLIELSEEGATQVFRPLNSNQLILGAVGILQFDVVAHRL
                     KHEYKVDCIYESVNVTCARWVYSEDDKAMSEFRTKAYDYLALDGGDMLMYLAPTKVNL
                     TMAEERYPKIKFCATREH"
BASE COUNT         1937 a         1298 c         1181 g         1846 t
ORIGIN      
        1 ttatgaggat ttcctatgaa tctgataaaa attaaatcta aaaccctatt catggtactt
       61 gtatacagtt tgctctcccc tgcttatgca gcatcacacc ccaaaagcac agtgaatgac
      121 accgccattc aaaatgaaat attagtttat atcaataatt accgacaaca gcatggcctg
      181 gctcctctca aaatggataa ccgcatagta aaagaagcaa aaattcattc catggatatg
      241 gcaaaacacg cgataccttt cggccataaa tattttaaaa aacgtattga taaattacac
      301 acacaaatta aaaactcaaa tgcaggggcc gaaaacgtgg cttacaacta taaaaatgcc
      361 caggatgttg tcaaaaactg gttacgtagc ccaggacaca aaagaaatat cgttggtaac
      421 tatgacctta ccggggtagg tatagctcga gatgaaaagg gaaaaatata ttttactcaa
      481 atattcttaa aaacaggaaa tagccactat gcctccagaa aacccttccc atcaattttt
      541 agtggtgctc tcttcagtaa gaaagcataa aaattaaaat agggtgttta acaaaatttt
      601 tgcaattttt aagtgcaatt gagtggaatt aattacaacg ttagcctaat attgaaacat
      661 agacggcaat cattgttcag ccaacagtac aattttggag gccagattga gagtgtggtg
      721 agcaagctca gcctagactg agaagccaaa agcactccgg aggaatccac agttttaaac
      781 aggactgcac tcaatcttgc tgtctataac atactaacac catggggcgt attcaatacg
      841 ccctttttat tgcttcattc ctcgcttgtc cggatccaaa gatccaaaat tggtatttta
      901 tgctgtagaa aattattcta aattacaata ggatagaata ataacggtaa taaggatttt
      961 cccatgatga ttgcaacctt aatggaatca gctcaggaaa cgcgagtagc cattactccc
     1021 aactcggcca aacaatacat aaaactgggc tatgatgtag ggattgaaaa aaacgcgggg
     1081 cttagctcag attttgctaa cgaagaattt gaaaaagctg gcgcaataat attcgatagt
     1141 aaaagtacat tactcaaaaa aacacaaatt cttttatgtg ttaacgaacc tgatcccaaa
     1201 aacctgaatg gcttacctcc agattctcta ataatcggac atatagacaa taacccactt
     1261 agcccgcttg ttaagtggtg tctggatcag aaaataacat tattttcaat gagtcttatt
     1321 cccaggataa gtcgtgctca aagcatggac agcctatcgt ctcaggctaa cctggctggt
     1381 tacagggcag ttttggaagc tgcgacaaca tttcatagag ctatacctat gatgatgaca
     1441 gcagcaggca tgatacaacc ggccaaagta cttattcttg gtgccggagt tgcaggcttg
     1501 caagccatag ctactgcgaa acgtttaggt gcagtggttt atgcctttga cgtaagaaag
     1561 gcagccaaag aacaggttga aagtttaggc gctgaattca tagaggtaat gcaggagcaa
     1621 gatagtgaaa cagctggggg atatgccaca gaaacaagcg aagaatataa aaaacttcag
     1681 gctgaactca ttgatcaata tgcaaaaact gcagacattg taatttctac tgccttgata
     1741 ccagggcgtc aagctccggt tttactttat aaaaaaactg ttgagcaaat gaaaccaggt
     1801 tctgtcattg ttgatctcgc aacgtctcga ggtggaaatt gtgaactcag tgttgccgat
     1861 aaaatcatca agcatggtga cattactata gtcggattta gcaatatggc aggtctcgtc
     1921 cctgctacgg caagtgacct ttattctaat aatctggttc atcttataaa ccttttggca
     1981 aaaaatccgg ctgaatttaa cttaaatcct gaagatgaga ttatccagca agcagttctt
     2041 tgtcataaag gaacttactt gccctttcaa ggagtaaagg agaagcaaaa tgcctgagat
     2101 tcatacactt gataatcctt atattacaat attaaccatt ttcgtactgg cctgttttgt
     2161 aggttattat gtggtttgga aagtaacacc agctttacat acacccctaa tgtcagtaac
     2221 caatgccata tccagtatta ttatacttgg tgctttaatt gctgcaggaa gtgaattaat
     2281 tggaagcatc acctggttag gtggcatagc catattcatt acttcaatta atatttttgg
     2341 tggctttgta gtaactcaac gcatgcttcg catgtataaa aaataatgat ttaaaggacg
     2401 gaatcagtaa tgaattcaat agttccactg ttttatttat tggcagccat ttgctttata
     2461 ttggctttaa aaggactggc cagtccagct acttcaagaa gagggaacct tctaggcatt
     2521 ctgggtatga tcctggcagt gggctccact ctgatgatgc ccactaccta ccatcatatt
     2581 cttttaatcg ccttaatagc aggcggcgga ataataggaa ctattattgc tttgaagatc
     2641 aacatgacgg ccataccaca actggttgct gcttttcatt ctttagtagg catggcagca
     2701 gttctggtag cctattgtgc ctttttatcc cccgcgtcct ttcaaatcgg cacacctggt
     2761 gctattgcta ccgcaagtct aattgaaatg agcctggggt taatcatcgg agccattacc
     2821 ttttctggtt caatcattgc ctttttaaaa ttgcaaggac tcatatcagg tattccaatt
     2881 cgattttatg ctcaaaacta tgtcaactta ctaataggac taacaatcct ggtgttactc
     2941 agtttatttg tagccaatca aaacctgttt ctctttaacc tgctcgcggg attagctttt
     3001 ctcattggtg ttttactgat tattcctatt ggcggagctg atatgcccgt cgttatttcc
     3061 atgctgaact cctactcagg ttgggccgca gcaggaatag gtttcactct gagtaatcac
     3121 ctgttagtta tcacaggcgc tctggtaggc gccagcgggg caattttaag ctatatcatg
     3181 tgcgttggta tgaatcgctc tatttttaat gtcatttttg gtggaataaa cagttctgga
     3241 aatacgaaac aagctcattc tgctaatgag ttacgcaatg tcaggcaagc taacggcgaa
     3301 gatgctgcat tcctattaag taatgccaag gatgtcatca ttgttcctgg atatggaatg
     3361 gcagtggcac atgctcaaca tgcggtgaaa gagcttgttg atgccttgga atctcgttct
     3421 gttcgtgttc gttttgcaat acaccctgtt gccggacgca tgcctggcca tatgaacgtg
     3481 ttacttgctg aagccaatat cccctatgac agagtctttg agcaaagtga aattaacaga
     3541 gattttgcta cctgtgacgt tgcttacgtg attggagcta acgatatcac caaccctgca
     3601 gctaaaactg atcctgcctc ccccatttat gggatgcctg tcctggaggt tgaaaaagct
     3661 aaaacggtct tattcgtcaa acgcagctta tctcctggtt atgcaggtgt ggaaaacgat
     3721 ttattttttc acgacaatac ctacatgctt ttcggagatg ccaaagtaat gaccgaatca
     3781 atagctaagg ctctaggaga aatataaccc tttcaaaata aaagcaataa tctctgacgg
     3841 ggtgggtacc cggttcaatc gacgcaaatg ccttttaatt tgccaacgaa gcaaaataaa
     3901 tgtatcttag ccagttaatt tttcataatg gagatatatc tatgctacga aaaatcggct
     3961 ttacgttgtt atgcgcagtg gcaacattta gttcaagcac ttattctagc gtgactcaca
     4021 cactccaagc tggagtaacg ctggaatatg agtttccccc taatgaacct ctagtctttg
     4081 ttaattattt atattgggaa gtagcggccg attgtaaaat taccagtgaa gatccatcca
     4141 atgaactttt tgccgaggca ctcgcaaaag ccggtaaaat taatggtgtt acgctctcta
     4201 aaggaaatac catgaaagtg actgtccatt ccggcgatat tttaaaatta agcgcggaat
     4261 ctggagcaaa agttcaaatc accaattttg gaccacatac tctcaaagca acttgtactg
     4321 cataaagaat tgagctaatc gcaccccaac actaaacagt aatggggtgt gaaaattcaa
     4381 atcctgttca tcagtgttct cgagtagcgc agaacttaat cttgggataa cgttcttctg
     4441 ccattgtcaa attcactttg gtaggcgcca ggtacataag catatctcct ccgtccaaag
     4501 ccaaataatc ataagccttg gtacgaaact cgctcattgc tttatcatct tcagaataaa
     4561 cccatcgagc gcaagtcaca ttgactgatt cataaataca atccacttta tattcatgtt
     4621 tcagtctatg tgctaccaca tcaaattgta atatcccaac agctcccaat attagctgat
     4681 tactgtttaa tggcctaaaa acctgggtag caccctcttc agataattcg attaatcctt
     4741 taagcaaggc tttactcttt aatgggtccc tcaacctgac aagcctaaac aattcaggag
     4801 caaaattagg aattccagta aactttaatt gttcaccttg tgtaaaagta tcaccaatac
     4861 gaatagtacc atgattatgc aaaccaataa tatcaccagc taaagcaagt tctgtgtgtg
     4921 atctatcgcc tgccataaaa gttaaggcat tggaaatttg cacctctttt cctatgcgta
     4981 aatgacttaa tttcattccc ttcttgtaag cgccagaaca aaccctcaga aaggcaattc
     5041 ggtctctgtg tttgggatcc atattggctt gaattttaaa aacaaaaccg gaaaatgttt
     5101 cttcattcgg agaaacaact ctctcctggg ttgctcgagg ttgaggtcct ggcgcatact
     5161 cgacaaaatc gtccaataat tctttaatac caaaattatt aattgctgaa ccaaagtata
     5221 ccggggtcat ttttcctgca agataatctt ccaagttaaa cccgtgggac gctcctttga
     5281 ctaactcaat ttcttctctg agttcgtctg cactgtcacc tatcaactca tctaattgag
     5341 gattatccaa tcctttaatc tgcatagctt ccactttttt tgcattactg ccttgctgat
     5401 ataaataaat aatatcttga tagcggtgat aaattccctt gaagcgttta cccattccaa
     5461 ctggccaggt aattggcgca cattgaatgc ccaaaacaga ctctacttca tctaacaaat
     5521 caataggttc tcgcccttct ctgtccaatt tattgataaa agtcatgatc ggtgtatcac
     5581 gtaaacggca aacttccatc agttttaccg ttcgctcctc tactcccttg gccacgtcaa
     5641 ttaccattaa ggcagaatct acagcagtga gagtacgata ggtatcttca gaaaagtctt
     5701 catggcctgg tgtatctaac aaattgatga cgtgttgatt atgtataaac tgcataacag
     5761 acgtggtaat cgatattcct ctctcttttt ccatttccat ccaatcagaa gtggcatgtc
     5821 tgtctgcttt acgacctttg accgtacctg ccaactgaat agctcctcca aaaagcaata
     5881 acttttcagt tacagtggtc tttcctgcat cagggtgaga aataatggca aaagttcgcc
     5941 ttttattaaa gtcttgataa aaatcagaca taggaatctc ggattaagtg gttaaatgcc
     6001 ttgaattgta aaggaaaagt acaaaactaa caattttttc gttaataatt cgcaagccat
     6061 aagtgtttcg tcaatttgga tcgttattaa tccacaaaat acaattaaaa atctacttaa
     6121 ccattaattg atcaatttgg ggtttattga tataatgcgc gcttttttgt ggagacctat
     6181 atgtcaacat cagaatcaga attaagaaaa tcaatgcaac aatatggaaa gcaggcattc
     6241 agtgaaacag aacaaataaa aa
//
LOCUS       DASLOE010000163         6249 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.164, whole genome shotgun sequence.
ACCESSION   DASLOE010000163 DASLOE010000000
VERSION     DASLOE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.164"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1612
                     /locus_tag="U6309_002465"
     CDS             <1..1612
                     /locus_tag="U6309_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEN5505894.1"
                     /translation="ALSGTIRTSSMMIISLICCYGLLWMLARCSEKIREMLSFVNIST
                     TIADYSTHVFAHLHALNYKFHLDKETGKITSAISRAQLAIAMLISNILFRIVPVFLEI
                     LFAFLIIWHFYGLSYGLYLIIILGIYLVFNGLTQKKSTVLQKNCSIAELNVSSAITDS
                     LLNTETVKYYNSQTVEHKKIDQFLQESVHANTALFSGNGTFLVIQTLIIAGGLAFLSY
                     KVGYQILIGQLKIGDFVLINGYLLQLFEPLSEASVRWRDTKNDLSKIEYSAYLLDQTD
                     EVEQDHPDAKPLFITGPHVHFKNVTFGYQPEHPILKNVSFEVPPAKMTAIVGPSGSGK
                     STIARLLLGLFEVTQGQILIDDQDITAVSKHSLRQQIGIVPQEVLLFNNTLRFNLCYG
                     ALNISEREINNVIQLAHLEEMVKNLPKGIETNIGEGGFKLSGGERQRIGIARCLLRNP
                     SILLFDEATASLDTQTEKNIQENIEEVTKHTTSIVIAHRLSTIIHADNILVLQDGEIV
                     EAGTHQELIEKKGLYYSMWNSQQQKSAS"
     gene            1609..3417
                     /locus_tag="U6309_002466"
     CDS             1609..3417
                     /locus_tag="U6309_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEN5505895.1"
                     /translation="MMNESLPSIKETIYYLWPCVWTKNSLRGKIHVVLALLFILISIA
                     LNLCVPIFLKEAINALSGHALFLNSTPLVIILTYASVWGASKACITLSQVIAFPVELE
                     GARKFCLELFDHVQALSTKFHRERKSGEILTIIERSHSSAVDLIGRPIVMILPVLIEI
                     IFAMIFLSYFYDLFFGLVLLLMLVSYILLSYYTAQWIVKCRMKQNNEDASANAYLVES
                     LLHADTVKYFNTQQDEINTAAQKLQEKACADTRALNADAKIHLMQNVIVGMCVIIMLL
                     LAGSRVIAGSMNVGDFVLVHGYLFMFMLPLSMLGYRIRVTRDDLARFQTAIQLLKIPI
                     DIQDQPNARTLKYKEGKIQFDHVHFTYNEHRKILDDVSFIVEPGTTTAIVGSSGSGKS
                     TLARLIFRLYDLNSGSIQIDGQNISNIQHASLRAILGIVPQETALFNDTLRNNLIYGN
                     PHVTHDELADAIQAVHLDRFVAKLPDGLETRVGERGLKLSGGEKQRVAIARMLLKKPK
                     IFIFDEATSSLDMKTEKDIQTCLKQISINTTTLIIAHRLSTVTHADNILVLAKGVIIE
                     QGTHNELLNQNGAYAQLWKAQSQNKIDTGSINGFVP"
     gene            3499..4341
                     /locus_tag="U6309_002467"
     CDS             3499..4341
                     /locus_tag="U6309_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN5505896.1"
                     /translation="MNIKQNDTSTTPPILYGTAWKEDDTKRLVLQALNSGFRGIDTAN
                     QRRHYFEEAVGDAIDQFLTTSQKTRSDLFLQTKFTSVHGQDHRKPYDEFDSLTNQVKQ
                     SFASSLEHLQTDYIDAYILHGPSLSHGIIDADLEIWQAMEELVCSRKVKFLGISNVNM
                     VQVEELYRKVSIKPSFIQNRCFAITQWDQDVRTFSQKNKIIYQGFSLLTANQRYLLNP
                     HMQSLAVKYDKTIPQIIFRFANQVGILPLTGTTNQQHMDNDLNIYDFELTQEEIQYIE
                     NIGL"
     gene            4352..4852
                     /locus_tag="U6309_002468"
     CDS             4352..4852
                     /locus_tag="U6309_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505897.1"
                     /translation="MTIKIETPRMLLRLIKDEDLDQVAQLHADREVRKFFPDGTQDRE
                     QTKRRIKQIMNLYGDKGLPGFVIFNKVTHEFIGRCGFGPIETGEIEVGYLIVRKFWGM
                     GYASEALEALLEWSKININSDYIIAFAPLKHSASHRVMEKCGMEYYKDELGHGVECKF
                     YRSKNK"
     gene            4875..5657
                     /locus_tag="U6309_002469"
     CDS             4875..5657
                     /locus_tag="U6309_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEN5505898.1"
                     /translation="MTKITTDEAALKLAVLIDADNARAVIIDGLLTEIAGYGDAIVRR
                     IYGDFTLPSSAQWKKVLHKYAIKPVQQFAFTSGKNATDSALIIDAMDLLYTHRFGGFC
                     IVSSDSDFTSLANRIREEGLQVFGFGEKKTPEAFRNACNKFIFTEVLRPVAPPDQLIS
                     QSKKRKQPVKQAKANEPSAQEFPKEFILEALNNSYDDTGWAYIGTFGGYLTKLKPDFD
                     SRLYGFKKLSDLVKGKPEIFETEERVNPGSNTKVLYLRGRQE"
     gene            5672..6049
                     /locus_tag="U6309_002470"
     CDS             5672..6049
                     /locus_tag="U6309_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate hydrolase"
                     /protein_id="HEN5505899.1"
                     /translation="MNLLEKIAALENEATEFGFQWETTDQIMTQIQSECIEVDEHLKA
                     GINDNSSALQEEIGDLLHAVFSLCVFCKLDLKDTLQQTVEKFERRLRAVKMIAHEMKL
                     INLEGQTFDELMSIWKQAKSRVG"
BASE COUNT         2068 a         1131 c         1219 g         1831 t
ORIGIN      
        1 tgcattatcc ggtacgataa ggacatcgag catgatgatt atctcgctga tctgctgtta
       61 tggattactc tggatgctgg caagatgcag tgaaaaaatt cgcgagatgc tttcttttgt
      121 aaacataagt acaacaattg cggattatag tactcatgtc tttgcacacc ttcatgcttt
      181 aaattacaaa tttcatctgg ataaggaaac cggaaaaata accagcgcta tcagtcgcgc
      241 acagcttgca atcgcgatgc ttatctcgaa catattgttt cggattgtgc ctgttttttt
      301 agaaattctc ttcgcttttt taatcatttg gcacttctat ggcttaagct atggcctcta
      361 tctaattatt attttgggaa tctatcttgt ttttaatgga ctcactcaaa aaaaatccac
      421 agtcctgcaa aaaaattgta gcattgctga attaaatgta agttccgcaa ttaccgacag
      481 cctactcaac acagaaactg taaaatacta caacagtcaa accgtagaac ataaaaaaat
      541 agaccaattt ttacaagaaa gcgttcatgc aaatacagca ttatttagtg gaaacggtac
      601 atttttggtg atacaaacac tcattattgc ggggggtctt gcctttttgt cctataaagt
      661 agggtaccaa attttaattg gacagttaaa aattggtgac tttgtcctga ttaatggcta
      721 tttattacag ttatttgaac ctttaagcga agctagtgtg cgttggcgag ataccaaaaa
      781 tgatttatcc aaaatagaat attcagccta tttattagat caaaccgatg aagttgaaca
      841 agaccatcct gatgcaaaac ctttattcat taccggcccg catgttcact ttaaaaacgt
      901 cactttcggt taccaaccag aacaccccat tttaaaaaat gtaagcttcg aagttccacc
      961 cgcaaagatg acagctattg tgggacccag cggatcgggt aaatcaacga ttgcacgatt
     1021 attgctggga ttatttgaag tcactcaggg acaaatactc atagatgacc aggatattac
     1081 tgctgtgagc aagcattcat tacgccaaca aatcggaatt gttccacaag aagttctact
     1141 cttcaacaat acgttgaggt ttaatctctg ctacggtgct ctaaatatct ccgagcgtga
     1201 aatcaataac gtaatccaac tggcgcactt ggaagagatg gtaaaaaacc tccccaaagg
     1261 aattgaaacc aacatcggtg aagggggttt taaattatca ggaggagaac gccagaggat
     1321 tggaatagca agatgtttat tacgcaaccc gagtattttg ttatttgatg aagcaaccgc
     1381 atcgctcgac acgcagactg aaaaaaatat ccaggaaaat attgaggaag ttacaaaaca
     1441 taccaccagt atagtcattg cacatcgttt gagtacgatt attcatgctg acaacattct
     1501 tgttttacaa gacggagaaa tcgtagaagc tggaacgcat caagaattaa tcgagaaaaa
     1561 gggattatat tactccatgt ggaatagcca gcagcaaaag agtgcatcat gatgaatgaa
     1621 agtttaccca gcattaaaga aactatctac tatctttggc cttgcgtttg gacaaagaac
     1681 agcctaagag gaaaaattca cgttgtcttg gctctcttat ttatattgat ttccattgca
     1741 ctaaatttgt gtgtccccat ttttttaaaa gaagccatta atgcactctc tggacatgcc
     1801 ctatttttaa acagcacccc tttagtcatc attctgactt atgcatcggt ttggggtgca
     1861 tccaaagctt gtatcaccct cagtcaagta atagcttttc ctgtagaatt agaaggggcg
     1921 agaaaatttt gtttagaact ttttgatcac gtacaagcgc tttccacaaa atttcataga
     1981 gaacgtaaat caggcgaaat tcttacaatc atagaacgtt cacactcgtc tgctgttgat
     2041 cttattggtc gacctattgt gatgatattg ccggttctta tagaaataat ttttgctatg
     2101 atttttttga gctattttta tgatcttttt tttggattgg ttctattgtt gatgctggtt
     2161 tcttatatct tgctaagcta ttacaccgct caatggattg taaaatgcag aatgaagcaa
     2221 aacaatgaag atgcatctgc aaacgcctat ctggtggaaa gcctgttgca tgcggatacc
     2281 gtgaaatatt ttaataccca acaggatgaa atcaatacag ctgctcaaaa attacaagaa
     2341 aaggcctgtg cagatactcg agcattaaat gccgatgcaa aaattcatct tatgcaaaat
     2401 gtcattgtcg gtatgtgtgt tattattatg ctcttacttg ctggcagccg agtcattgca
     2461 gggagcatga atgtcggaga ttttgtactg gtccatggct atttattcat gttcatgctg
     2521 ccattatcca tgctaggcta tcgaattcgt gttacccgag atgatttagc tcgttttcaa
     2581 accgcgattc agttgctgaa aattcctatc gatattcaag accaacccaa tgccagaaca
     2641 cttaagtaca aagaaggaaa aatccaattt gatcacgtcc attttactta taatgaacat
     2701 cgaaaaattc ttgatgacgt tagctttata gttgaacccg gtaccactac tgcaatcgtt
     2761 ggatcctcag gatccggcaa atccactctc gcaagattaa tctttcggct ttacgatttg
     2821 aattcgggga gtattcagat tgatgggcaa aatattagca atatccaaca tgcatcactc
     2881 agagcaatac tcggtattgt tcctcaggaa actgctttat tcaatgatac cttaagaaat
     2941 aacctgatat atggaaatcc gcatgtcact catgatgaat tggcggatgc aatacaagca
     3001 gtacatcttg acaggttcgt ggcaaaactg cctgatgggt tagaaaccag agttggtgag
     3061 cgcggattaa aattgtctgg tggcgaaaaa cagcgagtgg ccatcgctag aatgctgctt
     3121 aaaaaaccta aaatttttat ttttgatgaa gcaacatctt cactggatat gaaaacagag
     3181 aaagacattc agacctgtct caaacagata tcgatcaaca caacaacact tattattgca
     3241 catagacttt ctacagtaac gcatgcagat aatattctgg tactcgctaa aggtgtgatt
     3301 attgagcaag gtacccacaa tgaactacta aatcaaaatg gtgcctatgc tcaattgtgg
     3361 aaagctcaaa gtcaaaataa aattgacaca gggtctatta atggctttgt tccttagctc
     3421 attatcctcc gaaaaatacc tctatatttt gaggaggata atgcttggtt atgctgtgac
     3481 accaatggag gcggcattat gaatataaaa caaaacgaca catcaaccac tccccctatt
     3541 ttatatggta cagcatggaa agaagatgac acaaaacgtt tggtgctcca ggcactcaac
     3601 tcaggatttc gagggattga tactgccaat caacgtaggc attattttga agaagccgtg
     3661 ggtgatgcaa ttgatcaatt tttaacaact agtcaaaaaa cacgcagtga tttatttcta
     3721 caaacaaaat ttacctctgt acatggccaa gatcatagaa aaccttatga tgaatttgat
     3781 tcattgacca accaggtcaa gcaatccttt gcaagctcac tagaacattt acagacagac
     3841 tacattgacg cctatatact tcatggccct tcactatctc atggaataat cgatgcagat
     3901 ttagaaattt ggcaggccat ggaagagctg gtgtgtagta gaaaggtaaa gtttctcggc
     3961 atttctaacg ttaatatggt acaagttgaa gaactatata gaaaagtctc tataaagccg
     4021 tcgtttatac aaaatagatg tttcgccata acacaatggg atcaagacgt taggacattt
     4081 agtcaaaaaa acaagattat ctatcaagga ttttctttgc tcactgcgaa tcagcggtat
     4141 ttattaaacc ctcacatgca atcacttgct gtaaaatatg ataaaaccat cccgcaaatt
     4201 atatttcgct ttgcaaacca agtggggata ttgcctttaa ccggcactac aaatcaacaa
     4261 cacatggata atgatttaaa catatatgac tttgagctta cacaagaaga aattcaatat
     4321 atagaaaata taggattata agtgaaccaa tatgaccatt aaaattgaaa caccaaggat
     4381 gcttttgcgt ttaattaagg atgaggactt agatcaagtt gctcagcttc atgcagaccg
     4441 agaagttaga aaattctttc ctgatggaac acaagatcgc gagcagacta agcgaagaat
     4501 aaaacagatt atgaaccttt atggagataa aggattgcct ggttttgtta tattcaataa
     4561 ggtgactcat gaattcattg gacgatgtgg atttgggcca attgaaacag gggaaattga
     4621 agttggatac cttattgtaa gaaaattttg gggtatgggt tatgcatctg aagcactaga
     4681 ggcacttctt gaatggagta aaataaatat caattctgat tatattattg cttttgcgcc
     4741 actcaaacat agtgcatcac atcgagtaat ggagaagtgt ggtatggaat attataaaga
     4801 tgaacttggt catggtgttg agtgtaaatt ttatcgttcc aaaaataaat aatttataaa
     4861 acaggtcaat tagcatgaca aaaattacaa cagatgaagc cgcgttaaaa ttagctgtac
     4921 ttattgatgc tgataatgct cgagcagtta ttattgatgg tcttttaact gagattgctg
     4981 gttatggaga tgctatcgta aggagaattt atggcgattt caccttacca tctagcgcac
     5041 aatggaaaaa ggtattgcat aaatacgcta taaaaccagt acagcagttt gcttttactt
     5101 ctggaaaaaa tgcaacagat agtgcactta ttattgatgc tatggatcta ctttatacgc
     5161 atcgttttgg tggtttttgt atcgtgtcaa gtgatagtga ttttacaagt cttgctaacc
     5221 gtattcgtga agaagggttg caagtttttg gatttggaga aaaaaaaaca ccagaggcat
     5281 ttcgtaatgc ctgcaataaa tttattttca ctgaagtact acgccctgta gcaccacctg
     5341 atcaattaat tagccagtca aaaaagagaa agcagcctgt taaacaagca aaagctaatg
     5401 aaccctcagc acaagaattt ccaaaagaat ttattctaga agcattaaat aactcatacg
     5461 atgatactgg ttgggcatac ataggaacat ttggtggcta tttaactaag ttaaaaccag
     5521 actttgattc caggctttat ggtttcaaaa agctgagtga tttggttaag ggaaaaccgg
     5581 agatttttga aacggaagaa cgagtcaatc ctggatcgaa cacaaaagtt ttatatttac
     5641 gtggtagaca agaataacgt ggaaataatt aatgaatcta ttagaaaaaa ttgcagcatt
     5701 agaaaatgaa gctactgaat ttggttttca gtgggaaacg accgatcaga ttatgactca
     5761 aatccaaagt gagtgtattg aagtcgatga gcatttaaaa gcaggaatta atgataactc
     5821 atctgctttg caggaagaaa taggtgacct attgcatgcc gtgttttcac tgtgtgtatt
     5881 ttgtaaatta gatctcaaag atacactgca acaaaccgtg gaaaaatttg agcggcggct
     5941 ccgcgctgtc aaaatgattg ctcatgaaat gaaactcatt aatttggaag gacaaacttt
     6001 tgatgagttg atgtctatct ggaaacaagc aaagagcaga gtaggttaac gctgggtgag
     6061 catgacttgt aaattgggta gccgaaatgt agattcagca taaaatcaca aacgcgcaat
     6121 tgtcctgcgc ttgataaaat attttcatgc tcgcataaat ttccgtacaa aattaatgaa
     6181 taaaatcaac ctataaaaag aagacaggtc gggtaacagg atcgagttgc cccgatcccg
     6241 tcccctaag
//
LOCUS       DASLOE010000164         6187 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.165, whole genome shotgun sequence.
ACCESSION   DASLOE010000164 DASLOE010000000
VERSION     DASLOE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.165"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..2124
                     /locus_tag="U6309_002471"
     CDS             <1..2124
                     /locus_tag="U6309_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEN5505900.1"
                     /translation="YREVINQYSAIESSLQTILNSLTNQANLFVEPGLQKSVSLLQND
                     VKTELKETSNQKLILSKNLEDYQNQLKKQISTRQSLSEYNIDSWAVILKKLIDIPNLF
                     YKYIKTLTIKVYDSYTWLDTFPAIFLWTVLIFILSLFYILNHVLKTLARDKERSRFTG
                     YLYGGILVLLQRNIPHLCFFAMLSFILYFTSISYANSQLLIKLIIVWLTFRVLILIAR
                     LILLESISDSSGKDVKLYYRLKWLLLFGGWTTALMTFSHLLPLSILLQDIFNRLFMLF
                     MLTVSLVAWKSKDVIPYLIRPLLKSKKRYFKNAISLLVILVPITLFTTAIIGLIGFVN
                     LAWTMSRYQAYILLVLVGYILVRGLIFDALELFSEWMISSLKNGWLWIEVFLKPIDKI
                     LRIVLFLTSVLILFQLFGWYSDSLVVTSLEKVVQYSILNITGIHITVASIIEFLILLA
                     VLVWAAKWTREFCFRWLYKNTKDVGIRHSLSVFTQYAIILLGTFITLHVLGLDFSGMS
                     MILGGLAVGMGFGLRDFASNIVGGIMLLIERPVREGDLITIGEYEGQVAHIGIRSMRV
                     SSWDNTQVLIPNAETFNKPFTNWTHQDGIIRTVIPIKVSRADDPVMIQQLILDVLAII
                     PEIVPDPPAQVFLKKIDAALIEFEARYFINIQNHNRVEVRSKVLFAITAQFKAAGIQP
                     PIEPITVELKESSSDFSLKKQTTEE"
     gene            2087..2761
                     /gene="mutH"
                     /locus_tag="U6309_002472"
     CDS             2087..2761
                     /gene="mutH"
                     /locus_tag="U6309_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HEN5505901.1"
                     /translation="MILALKNKPPKNEKELLEQCQRIEGLSFAQLALELGLFTPLNPV
                     HRKGWVGQALELALGADAKNESLPDFKQLGIELKTIPINKSGKPAESTFIVSIPLLTI
                     HQQTWESSQCYAKLKKVLWIPIEGDTDIPFPHRRIGCGFLWSPNKDQESVLAEDWNYL
                     TNQIVLGQLETLNSSSGEYLQVRPKAANGKSLCYGYDPEGNRIRTLPRGFYLRSCFTS
                     VILSQS"
     gene            complement(2834..3874)
                     /locus_tag="U6309_002473"
     CDS             complement(2834..3874)
                     /locus_tag="U6309_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505902.1"
                     /translation="MKTIFANKELLYNQYKKMFLEQISDINKLKECIKARYDIKELPD
                     EILFKLLTNNHKKIYDFIGTHNQNRIRYFFGNFDNFDPRDNQISVLDRFILDSVHFYI
                     TSSLLMQLHKEYQENAMEIDKAWQKEIQAHAHFGPLPTIAIIGNLFKSNKNHVFKDLQ
                     FPANKNIQLFQLNKLSIKLNDQVFALWLPKASEAQVSLSNFTRNTIFVCIATAAILAI
                     GVSLFVFSTSSAAGLAVIFNPASLGIMIALLACATLMLGISSLYSSTTIHDSTIEHPE
                     NRTPEKPYHSHNIRKDKIHSSKAKPEEQLPYHDLKWLLFNRKPYTLRESIVTPATASE
                     QISSMCTNRQKL"
     gene            complement(4073..4909)
                     /locus_tag="U6309_002474"
     CDS             complement(4073..4909)
                     /locus_tag="U6309_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505903.1"
                     /translation="MGKVNAVGMAEVGVERAKRKEALRVLTSSSFNLDNKEHTQALEK
                     YIKQFELSVNQMYLYQTMSTALTAWGGSWIVGRFLPIPEFANHLLAACLYLGVSGYIL
                     ESFSMTDFYEQLKEMKVLYNWCLKNNKENYSTDIDNTNNLLYPDIQRMIKLIAPLCST
                     DFMIAWPKEVKTAETQSGILSNTLSIGYSIVSAPFSLFSTASKPSQDQVQRIKDLKVQ
                     VETRGLDLGVFAGFEQAIKYFATNSDFRELLKAKLTQPVNSFKNLLQTTIPGMLAGSN
                     SK"
     gene            5055..>6187
                     /gene="legK1"
                     /locus_tag="U6309_002475"
     CDS             5055..>6187
                     /gene="legK1"
                     /locus_tag="U6309_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector kinase
                     LegK1"
                     /protein_id="HEN5505904.1"
                     /translation="MPRTMFFSVDAANLQNLQITNQKAFAALESYLNDNDSPGAWNNN
                     QGYKYTHKDITYCFSFNQSLVRRPRKSDQKKHTFEIFDPNKNPLGKGAYGIVYPILGT
                     IQFEFGNAIKRPPKNKLVKIQNHSEHDQSHSVLREYQGLLQSGHIAVKPPIFVENKES
                     KFSYLIMEKAEGIVLEKFLNPVKRLDLSEEVPELNLINRIEITFAILKAIKEQVTDKH
                     LIHRDIKPGNIIIDFSKSPPIAKVIDFGFVLRASEQDYRRCGTRAYRAPESFNTQALY
                     TAKADVWSTGRILSYLWGDKYTNYYISRDKGLDYVIEKSRNELLFSDPELELYLTKED
                     KSKIRSCLSTMLIVDPEERGSIDKAIKQFSQIDFEKYKRLKRST"
BASE COUNT         1930 a         1025 c         1155 g         2077 t
ORIGIN      
        1 tacagagagg ttattaatca atattcagct atagagagtt ctttgcaaac cattctgaat
       61 tctcttacaa atcaggccaa tctttttgtt gagccagggt tacaaaaatc cgttagctta
      121 ttgcagaatg acgttaaaac agaattaaag gagacctcta atcaaaaact tatcttatcc
      181 aaaaatttag aggattatca aaatcaatta aagaaacaga tctcaacaag acaaagctta
      241 agcgaataca atatagacag ttgggcagtc attctcaaga agctaattga tatccccaat
      301 ttgttttata aatacatcaa aacactaacc ataaaggttt atgatagtta tacctggcta
      361 gatacttttc ctgctatttt tttatggaca gttcttattt ttatcctgag cctgttttat
      421 attttaaatc atgttttgaa gactttagca agagataaag aacgctcaag atttactggt
      481 tatctttatg gtggaatact cgttttactg caaagaaaca tacctcatct ttgcttcttc
      541 gctatgttat ccttcatttt gtacttcacc agtatttctt atgctaactc tcaattatta
      601 attaaactca ttatcgtgtg gctcaccttt agagttttaa ttttaatagc ccgtttgatt
      661 ttgttagaaa gtatatcgga ttcatcaggt aaggacgtta aattatatta tcgactaaaa
      721 tggttgttgc tttttggtgg atggacaacc gcgttgatga catttagtca cttgttacct
      781 ttatcaattc tactgcaaga tatatttaat cgattattca tgctttttat gttaacggta
      841 tccttggtcg catggaaaag taaagatgta attccttatc tcattagacc attactgaaa
      901 tcaaagaaaa gatattttaa aaatgcaatt tctttattgg tcattttagt tcctataacc
      961 ttatttacaa ccgcaattat tggtttaatt ggctttgtga atctggcatg gactatgagt
     1021 cgttatcaag cttatattct gttggtttta gtaggttata ttctcgtaag aggtttgata
     1081 tttgacgcat tagagctttt ctctgagtgg atgatctctt cattgaaaaa tgggtggtta
     1141 tggatagagg ttttcctcaa accaatcgat aaaatcttgc gtattgtctt gtttttaaca
     1201 agtgtattaa ttttatttca gttgtttggt tggtactctg attcactggt tgtgactagc
     1261 ctcgaaaaag tagttcaata cagtattctt aatattacgg gcatacatat tacagtcgcc
     1321 agtatcattg aatttcttat tctacttgct gttttagtat gggcagcaaa gtggacaagg
     1381 gaattttgct ttcgatggct atataagaat accaaagatg tgggtatacg ccatagttta
     1441 tctgtattta cacagtatgc aatcatatta ctgggtactt tcatcactct tcatgtcctg
     1501 ggactggatt ttagcggtat gtcgatgata cttggtggac ttgctgtcgg tatgggtttc
     1561 ggtttaagag attttgcttc aaatattgtt ggaggcatta tgcttttaat tgaaagaccc
     1621 gtaagggaag gcgatttgat aactataggt gaatacgaag ggcaagtggc tcacattggc
     1681 attcgctcga tgcgcgtatc ttcatgggat aatactcagg ttttgattcc gaatgctgaa
     1741 acattcaata aaccttttac aaactggact catcaagatg gtattattcg tacggtcata
     1801 ccaataaagg taagcagagc cgatgatcca gtgatgattc aacagttaat acttgatgta
     1861 ttggcaatta ttccggaaat tgttccagat cctcctgcac aagttttttt aaagaaaatc
     1921 gatgcagctt taatagaatt tgaagcacgg tattttatta acatccagaa tcataaccgg
     1981 gttgaagtaa ggtcaaaagt cttgtttgcc ataactgcac agtttaaagc ggcaggaata
     2041 cagccgccaa ttgagcctat tactgttgaa ctaaaagaga gctccagtga ttttagcctt
     2101 aaaaaacaaa ccaccgaaga atgaaaaaga acttttggag caatgccagc gaattgaggg
     2161 gttgagtttc gctcagttag ctcttgagtt gggtctattt acccctttaa accccgttca
     2221 tcgaaaggga tgggtcggac aagctctcga gttagcttta ggcgctgatg caaaaaatga
     2281 gtcgctacct gatttcaagc agttgggcat tgaattaaaa acgataccta ttaataaatc
     2341 agggaaaccg gccgagtcca catttattgt ttctattcca ctactgacca tccatcagca
     2401 aacttgggag tcttctcaat gttacgctaa attaaaaaag gtattatgga ttcctataga
     2461 gggagataca gatattccct ttcctcacag aagaattgga tgtggatttt tatggtctcc
     2521 gaataaagat caggaatctg ttcttgcaga agattggaat tatttaacaa atcaaatcgt
     2581 tctgggacaa ttagaaacat taaattcctc ttcgggagag tatcttcaag tgaggcctaa
     2641 ggctgccaat ggaaaatctt tatgttatgg ttatgaccca gagggtaatc gtataagaac
     2701 gttaccacga ggattttatt tacgctcctg ttttacatcc gtaattttaa gtcaatccta
     2761 attttaagga taaattaagc accatctgtt taccggtatt atgataaatt ttacagatat
     2821 tagggaaagc ttattatagc ttttgtctat tagtacacat ggacgatatt tgttcagatg
     2881 ctgttgctgg agtcactatt gattctctta atgtatatgg ttttctatta aataataacc
     2941 atttcagatc atggtagggt aactgctctt ctggtttggc tttactagaa tgtattttat
     3001 ccttgcgaat attatgagag tgataaggtt tttctggtgt tctattctcc ggatgttcta
     3061 ttgtactgtc atgtatggta gtggaagaat aaagagagct tatacccagc attagcgtgg
     3121 cacatgctaa caatgcaatc attattccta gcgatgcagg attaaaaata acggctaacc
     3181 cagctgctga gcttgtagaa aatacaaaga gacttacgcc tattgctaaa atagcggcgg
     3241 tggcaataca aacaaaaatg gtattgcgag tgaagttgga tagtgatact tgtgcttcac
     3301 ttgctttggg taaccagagc gcaaagactt gatcattaag cttaattgat aatttattta
     3361 attgaaagag ttgtatgttt ttgttcgcag ggaattgtaa atctttaaag acgtggtttt
     3421 tatttgattt aaacaagttt ccaatgattg ctattgttgg taatggaccg aaatgggcat
     3481 gggcttgaat ttccttttgc catgctttat caatttccat tgcattctct tgatattctt
     3541 tgtgtaactg cattaataaa ctgctagtga tataaaaatg aacgctatca agaataaatc
     3601 gatctaaaac gctaatttgg ttatcacgtg gatcaaaatt atcaaaatta ccgaaaaaat
     3661 atcggattct attttgatta tgtgtcccta tgaaatcgta aattttttta tgattattag
     3721 ttaataattt aaaaagtatt tcgtcaggaa gctcttttat atcatacctt gctttaatac
     3781 attcttttaa tttgttgatg tcagatatct gctctagaaa catttttttg tattgattgt
     3841 aaagcaattc cttgtttgca aaaatagttt tcatcttgag aaacctttgt ggagtaaatc
     3901 aatgacatta tttgctgtta atcatcctca tattggcctt aatgatgatt atgcctcctt
     3961 tctttaatgc taatgccaac ctttaataag gcttaaggtc ggcaagaaaa acatcagctc
     4021 tttacttaat aggaccgtta ggtaatattt aagcatgagg gatctattac tattattttg
     4081 aattagatcc tgccaacatt cctggtatgg ttgtttgcaa caaattttta aatgagttga
     4141 caggttgagt tagcttggct ttcaatagct ctctaaaatc agagttcgta gcaaaatact
     4201 tgattgcctg ctcaaaacca gcaaaaaccc ctaagtcaag acctcttgtt tctacttgca
     4261 cttttaggtc tttaattcgc tgcacttgat cctgactcgg tttactggct gttgagaaca
     4321 gtgagaatgg tgctgatact atagaatagc ctatggacaa tgtgttggat aggattccac
     4381 tttgagtttc tgcggttttt acttctttag gccaggcaat cataaaatca gtagagcata
     4441 aaggggcgat taatttaatc atgcgctgga tatcaggata caaaaggtta ttggtattat
     4501 cgatatcggt actatagttt tctttgttat tttttagaca ccaattatag agcactttca
     4561 tctctttcaa ttgttcataa aaatctgtca tgctaaatga ttcaagaatg taaccagaaa
     4621 cgcctaaata gagacaggct gcaagaaggt gattcgcaaa ttcaggaata ggcaaaaacc
     4681 ttccaactat ccacgagccc ccccacgctg ttaatgcagt actcattgtt tgatagaggt
     4741 acatttgatt gacacttaac tcaaattgtt tgatgtactt ttccaatgcc tgagtgtgtt
     4801 ccttattatc caggttgaag gaggatgacg tcagaactct tagggcttct ttccttttgg
     4861 ctctttcaac tccgacttca gccattccaa ctgcgtttac cttacccaca attctctcct
     4921 tgaaatcaaa atttcatttt atactataaa tttaaaaaat aatgaattag attttagcta
     4981 agagttcaat taatttaact taataaaaaa gaggaggtat aataaaaccg attaatcaaa
     5041 ttgaagtcaa tatcatgcct cgtacaatgt ttttttccgt tgatgcagct aatctccaaa
     5101 atttacaaat tacaaatcaa aaagcttttg cagcgttgga gagttatctg aatgataatg
     5161 attcaccagg tgcctggaat aataatcaag gatacaaata tactcataag gatattactt
     5221 attgcttctc atttaatcaa tccttagtga gacgcccaag aaaatcggat caaaaaaaac
     5281 atacatttga aatttttgac cctaataaga accctttagg caagggagcg tacggtattg
     5341 tgtatcccat tttaggtaca attcagtttg aatttggaaa tgcaattaaa agaccaccaa
     5401 agaataaatt agttaaaata caaaaccata gtgaacacga tcaaagtcat tctgtactac
     5461 gtgaatatca agggcttctt caatcagggc atatagcggt taaaccgcca atatttgtag
     5521 aaaacaaaga gagtaaattc agttacttga ttatggaaaa agcggagggt atcgttttag
     5581 agaagtttct aaatcccgtt aagcggcttg atttgtctga agaggtacct gagcttaatc
     5641 ttattaatcg gattgagatt acttttgcca ttttaaaagc aattaaagag caggttacgg
     5701 ataagcattt gattcatcga gatataaaac caggaaatat tataatcgat tttagcaagt
     5761 cacctccgat agctaaagta atcgattttg gttttgtgct tagggcgtca gaacaggatt
     5821 atcgtcgttg tggaacccga gcatacagag cgcccgaatc tttcaatacc caggcattgt
     5881 acacagcaaa agctgatgtt tggtcaacag gcaggatatt aagttattta tggggagata
     5941 aatatactaa ttattatatc tctcgtgata agggtttgga ctacgtgatt gagaagtcaa
     6001 gaaacgaact acttttctct gacccagaat tggaactcta tttaaccaaa gaagataaaa
     6061 gtaaaatcag atcgtgccta agcacaatgc ttatagttga ccctgaggaa agaggatcta
     6121 ttgataaggc tatcaagcag ttttcccaaa ttgattttga aaaatataaa agattaaagc
     6181 gctctac
//
LOCUS       DASLOE010000165         6053 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.166, whole genome shotgun sequence.
ACCESSION   DASLOE010000165 DASLOE010000000
VERSION     DASLOE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.166"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..168)
                     /locus_tag="U6309_002476"
     CDS             complement(<1..168)
                     /locus_tag="U6309_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505905.1"
                     /translation="MDIKKLKKAASNFLERYPGGFYHPEMIEMGKKHKMDKMVALAQG
                     SFAKQNFKNPDE"
     gene            complement(243..1067)
                     /gene="mutM"
                     /locus_tag="U6309_002477"
     CDS             complement(243..1067)
                     /gene="mutM"
                     /locus_tag="U6309_002477"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEN5505906.1"
                     /translation="MPELPEVETTKQGIKPHLEGRMITAVQVRNRKLRLPVPLNLNEL
                     CQGKHITAITRRGKYILLHMDKGYLLIHLGMSGHLRIVSQTANPQKHDHVDLHINNGL
                     ALRFCDPRRFGLFIYIDENPYQHPLLAHLGPEPLSDDFNSEYLLRKAANKSQSIKSFI
                     MDSQIVVGIGNIYAAESLFLAKIHPNTSAKKITTEEFNSLTGHIKKILESAIEAGGTT
                     LRDFYSSDGKPGYFRFALKVYGRKNLPCLVCENKIETVVIAGRHSAFCPHCQPIIT"
     gene            complement(1067..1297)
                     /locus_tag="U6309_002478"
     CDS             complement(1067..1297)
                     /locus_tag="U6309_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505907.1"
                     /translation="MSDKVQKLIENIVPFIVIGVGIAIVIALLFMFFYVAIWGVIIGG
                     ILWLGALAKEYFFPSKSSQKEQGRIIEHDDKK"
     gene            1478..2665
                     /locus_tag="U6309_002479"
     CDS             1478..2665
                     /locus_tag="U6309_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEN5505908.1"
                     /translation="MVFGFLNLSFWGYVLATFILTQITIASVTIYLHRNQTHRALTLH
                     PVVSHFFRFWLWLTTGMVTADWVAIHRKHHATTDVEGDPHSPVVEGIRKVFWQGAELY
                     REARKDRDMVAKYSHGTPTDWIERNVYSRHSSKGILLMLLLDLFFFGIPGMTVWAIQM
                     MWIPLFAAGVINGIGHYWGYRNFECPDAATNIIPWGFWIGGEELHNNHHTFASSAKFS
                     VKWWEFDIGWMYIRILSLFGLAKVKKLPPKLAMDEGKFHVDLDTVRAVISNRFQVMSN
                     YYKNVIRPILKYEKNTGIETKEDKKLFQRAGRLLRREDKLLSTKAKTRLQKLLDAREQ
                     LRVVYSYKQSLQNVWLKTATTQKELVEALQQWCRQAEESGLDVLKQFAQQLKGYVPVY
                     TRE"
     gene            complement(2707..3105)
                     /locus_tag="U6309_002480"
     CDS             complement(2707..3105)
                     /locus_tag="U6309_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505909.1"
                     /translation="MFNKFLVTTLSVVTLTLSPYGFAKDDSCEVQVAVSTPPVQFDQS
                     VAFNVTNDDGISKSIILQGGSSPKTIGKLICSPLPYTISATQYFTPTGLTNKHAQPVG
                     QCVLKAGNVILNGSNNSVAVVFPNDFICHH"
     gene            3371..4111
                     /gene="phbB"
                     /locus_tag="U6309_002481"
     CDS             3371..4111
                     /gene="phbB"
                     /locus_tag="U6309_002481"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEN5505910.1"
                     /translation="MDKMIAIVTGGTGGIGSAISQRLADSYQVVACYYKDGRHEEAKK
                     WQDEQRQLGYDIDIVYGDIAQYSDCEKITSLVMERYGRIDVLVNNAGITKDCSLRKMT
                     PQQWQQVLDANLTSVFNMTRNVVPVMLERGYGRIVSISSINGRKGQFGQCNYASTKAA
                     LFGFTKSLALEVASKGITVNTVSPGYIETPMLAALKEDVLNSIISSIPVGRLGYPKEI
                     ADAVAFLASPDSGFITGANLDVNGGQYM"
     gene            4290..5036
                     /gene="phbB"
                     /locus_tag="U6309_002482"
     CDS             4290..5036
                     /gene="phbB"
                     /locus_tag="U6309_002482"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEN5505911.1"
                     /translation="MLNNNVVLITGGTGDIGTAVAKQLDSTFKHVIALDLVSYDEGEA
                     WLHDLKTQGYKNINFRHMDVTDYEQCAEVIGLIIEEFGGIDVLINNAGITRDAVFTKM
                     TKRQWDEVLRVNLDGMFNVTKQVVESMKAQESGRIVNVSSVNAQKGQFSQANYAASKA
                     GVYGFTKSLAQELMIKNITVNSISPGYVNTRLMKGIRPDILEGIINLIPAKRLAEPQE
                     IAWAIEFLISEKSRYITGANLSVNGGLHMY"
     gene            5166..5564
                     /locus_tag="U6309_002483"
     CDS             5166..5564
                     /locus_tag="U6309_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate biosynthesis repressor
                     PhaR"
                     /protein_id="HEN5505912.1"
                     /translation="MTRLIKKYKNRRLYDTEISQYITVEQLQKYVVDGIDFKIEDSET
                     GKDLTNSVLLQIIVEMEAGPTQFLSSEILRQIISLANHPMHVSFKEMMEQMFHAMDKP
                     LQNNPYQQATEAWNKQMQQMVHQWQKMFKV"
     gene            5772..>6053
                     /locus_tag="U6309_002484"
     CDS             5772..>6053
                     /locus_tag="U6309_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEN5505913.1"
                     /translation="MTQYNFERWSEMAKKIQEPFQAIAELNVKTLQGLSYLKPDELAQ
                     VKKPEEMLEKQINLAVENGHKALDYMQKSFQIIEKAMLSFVQEAKKTTAE"
BASE COUNT         1959 a         1001 c         1243 g         1850 t
ORIGIN      
        1 ttcatcaggg tttttaaaat tttgcttggc aaaactccct tgagccaaag caaccatctt
       61 atccatttta tgttttttac ccatttcaat catttcaggg tggtagaaac cgcctggata
      121 tctttccaga aaattagagg ctgctttttt aagtttttta atatccataa attaaccttt
      181 tgtcacaaca acagaatcaa ttaagaaaca aaaatggttc caggtggcca ttattaaact
      241 tattatgtaa tgattggctg gcaatgtggg caaaatgcgg agtggcgccc ggcaatcacg
      301 acagtttcta ttttattttc acataccaaa caggggagat ttttcctgcc ataaaccttt
      361 aaagcaaaac gaaagtaccc tggtttacca tcagaagaat agaaatcacg aagtgtcgtc
      421 cctccagcct ctatggcaga ttcaagtatc tttttgatgt gaccagtcag agaattgaat
      481 tcttctgtag ttatcttttt tgcagaggta tttggatgga ttttggcaag aaaaagactc
      541 tctgccgcat aaatattccc tatcccgaca acaatttgac tatccataat aaatgacttg
      601 atggattgcg atttattcgc agctttacgt aataagtact cgctattaaa gtcatcagac
      661 agaggttccg ggcctaaatg tgccagcagt gggtgttggt aaggattttc atcaatgtaa
      721 ataaataagc caaatcttct aggatcgcag aatctgagag caagcccatt attaatgtgc
      781 agatcaacat gatcatgttt ttgaggattt gctgtctggg atacgatacg caaatgtccg
      841 gacattccaa gatggatgag aagataacct ttatccatat gaagtaagat gtattttcct
      901 ctacgggtaa tagctgttat atgctttcct tggcataatt cattcaggtt tagaggtact
      961 ggtaatctaa gttttcgatt acgcacttgc actgcggtaa tcatacggcc ttcaagatgg
     1021 ggtttaatac cttgctttgt tgtttcgact tcgggtaatt caggcattat tttttatcat
     1081 catgttctat aattcttcct tgttctttct gggatgattt gctggggaag aaatattcct
     1141 tagctaaggc cccaagccag agtattccac cgatgattac gccccaaatg gccacataaa
     1201 aaaacatgaa caataaggct ataactattg cgatcccaac accaatcact ataaaaggca
     1261 caatattttc aatcaatttc tgaactttat cgctcatgtc tatatcctga taaaataaga
     1321 tgagtaaatt tattatcgac tacatcatga aatcatgcaa tgaaatgata aaaaatgtgt
     1381 tatcattaca catatattga ttctataatt taggaataaa atacattcac tcttaaatta
     1441 tagttatttt tataattccg tacaatgtgg ggttattatg gtattcggct ttttaaacct
     1501 atctttctgg ggatatgttc ttgcaacttt tatcctgact caaatcacaa ttgcatctgt
     1561 tactatctat ctgcatagga accagactca tagagcgcta acattacacc ctgtggtcag
     1621 tcattttttc cgtttttggt tatggttaac tacgggtatg gttactgcgg attgggttgc
     1681 tatccatcgc aagcaccatg caacaactga tgttgaggga gaccctcata gcccagtggt
     1741 tgagggaatc aggaaggtat tttggcaagg cgctgaactt tacagagagg cacgcaagga
     1801 cagggatatg gttgccaaat attctcatgg cacgccaacc gattggatag agcgtaatgt
     1861 ctactcgcgc cattcaagta aaggtatttt gctgatgtta ttactggatt tatttttctt
     1921 cgggattcct ggaatgactg tatgggcaat ccaaatgatg tggattccgc tgtttgctgc
     1981 gggagtcatc aatggcatcg gccattattg gggatacagg aattttgaat gtcctgatgc
     2041 tgctactaat atcattccct ggggattttg gattggtgga gaagagttac ataataatca
     2101 tcacactttt gcgtcctcag ccaaattttc agtgaaatgg tgggagtttg atattggttg
     2161 gatgtatatt cgcattcttt ccttgttcgg tttggccaag gtaaaaaaat tacctcccaa
     2221 attggctatg gatgaaggaa aattccatgt tgatctggat actgtgagag cagttatttc
     2281 aaatcgtttt caagtgatgt ctaattacta taagaatgtc atcagaccta ttttgaagta
     2341 tgaaaaaaat accggcattg agacaaaaga agacaagaaa ttgtttcaac gggcaggtag
     2401 attactgcgt cgagaggata agctattatc gacaaaagct aaaactcgtt tgcaaaaact
     2461 gctcgatgcc cgcgaacagt tgagagtggt ttactcttac aagcaatcat tacaaaatgt
     2521 ttggctgaaa acagcaacga ctcaaaaaga attagtagaa gctctgcagc aatggtgccg
     2581 ccaggcagag gaatcgggtc ttgatgtgct gaaacagttt gctcaacaat tgaaaggcta
     2641 cgtaccggta tataccaggg agtgatgttt ttgcactccc catcaatgca caatgcagct
     2701 aatattttag tggtggcata taaaatcatt ggggaaaaca acagcaacgc tattgttaga
     2761 accattcaat atgacatttc cagcttttaa tacgcattgg ccaacaggtt gagcatgctt
     2821 attggttaag cctgttggtg taaaatattg ggtggcgcta atggtataag gcagtggaga
     2881 acaaattaat ttccctatag tttttggact gcttccgcct tgtaaaataa tggatttact
     2941 aatcccatca tcattcgtta cgttaaacgc cacactttga tcaaattgaa cagggggagt
     3001 ggaaacagcc acttgaactt cgcatgaatc atctttggca aaaccatatg gtgaaagtgt
     3061 caatgtgact acggatagtg ttgtaactaa aaatttatta aacataatac cctcattttc
     3121 tgaatgctac ttctaatatt aaaaaacact tatattacga tgcttactta aaagcagatt
     3181 tagggtatct tcattcgatt tgtaatgcaa attagtcatc attaatacat ttatatcaga
     3241 tccgtaacat gatcaaaaat aaagtagctg aattattatc accaacttag tattatatat
     3301 atgtcattga caagaaagtg aaattagatt gtagtaatgt aatgttacct agcaacaaaa
     3361 ggagttgtcg atggataaaa tgatagcgat tgtaactggg ggaacaggcg gtattggttc
     3421 tgctattagt cagcgcttag ctgatagtta ccaggttgtg gcatgctact ataaagatgg
     3481 aagacatgaa gaagcaaaaa aatggcaaga tgagcaaagg caacttggtt atgacattga
     3541 tatcgtatat ggagatatag ctcaatatag tgattgcgaa aaaatcacat ccctggtgat
     3601 ggagcgttat ggccgtatag atgttttggt taacaacgca ggcattacta aagactgcag
     3661 tttaagaaaa atgacacctc aacaatggca acaggtttta gacgccaact tgaccagtgt
     3721 attcaatatg acgcgcaatg tcgttccagt gatgttggaa agaggttatg gacgtatcgt
     3781 cagtatatct tcaatcaatg gaagaaaagg gcagtttggc caatgcaatt atgcttccac
     3841 caaggcggct ttatttggtt ttaccaagag tcttgcatta gaagtagcaa gtaaaggtat
     3901 tacggtaaac acagtgtcgc ctgggtatat tgaaactcct atgctcgctg cattgaaaga
     3961 agatgtactc aattcaatca tatccagtat tcctgttggt cgattgggat acccaaaaga
     4021 aattgcagat gccgttgcat ttttagcatc accagatagc ggatttatta cgggggcaaa
     4081 tctggacgtc aatggtggtc agtatatgta gtcagagttt gattcaccga ttaagaatct
     4141 caagaggttt tgtgaacata ttatttactg ttcgcaaggc accttgggat tttgaaaaag
     4201 tggctgcgat caccgttaac aatgaataaa atataagctc caattttaaa tcatcaattg
     4261 gggataggga aaaactaata gagaaattca tgctgaataa taatgtggta ttaattactg
     4321 gtggaacagg tgacattggt acggctgttg caaaacaact ggattcaact tttaaacatg
     4381 tcattgcttt ggatttggtt tcctatgatg agggtgaagc atggcttcat gatttgaaga
     4441 cacaaggcta taaaaatatt aatttcaggc atatggatgt aactgattat gagcaatgtg
     4501 ctgaagtaat aggcttaatt attgaagagt ttggtggtat tgatgtgctg attaataatg
     4561 ctggaattac tcgagatgct gtgtttacca aaatgaccaa aaggcaatgg gatgaagtgt
     4621 tacgtgtcaa tcttgatgga atgtttaatg taactaaaca agtagtagag agtatgaaag
     4681 ctcaggagtc aggccgaata gtgaatgtgt cttcggttaa tgcccagaaa gggcaatttt
     4741 cccaagccaa ttatgctgct tcaaaggctg gagtatatgg ttttaccaaa agtcttgccc
     4801 aggaactaat gattaaaaat attacagtaa acagtatttc tcctggttac gttaatactc
     4861 gtctgatgaa aggtattaga ccagatattc tggaggggat tattaatttg atacccgcaa
     4921 aaagattggc agagccacaa gaaattgctt gggctattga atttctgatt agtgagaaaa
     4981 gtcgatatat cacgggagca aatcttagcg ttaatggtgg tttgcatatg tattaacttt
     5041 tttaaccttg aaagaatttg taaaagaagt gtgaaagatt tactcttggg cttaaagaag
     5101 caaactatac caattttgca catcatttta taattatatt tctatatctg atacttgaga
     5161 aaataatgac tcgacttata aaaaaataca aaaatcgaag actgtatgat actgaaatta
     5221 gtcaatatat tactgttgag caactacaaa agtatgttgt agatggtatt gatttcaaga
     5281 tagaagattc agagacagga aaggatttaa ctaattcggt tctgttgcaa attatagtgg
     5341 aaatggaggc gggaccaacg caatttttgt cttcagaaat tttacgtcaa atcatttcat
     5401 tagctaatca tccaatgcat gtttccttta aggaaatgat ggaacaaatg tttcatgcaa
     5461 tggacaaacc tttacaaaac aatccttacc agcaagcgac tgaagcctgg aataagcaaa
     5521 tgcaacaaat ggtgcatcaa tggcaaaaaa tgtttaaagt ctaaaaattc ctcccaaact
     5581 tttttgtagt taatagaacg tttcgtttta cgagttttac tggtgatttg gaaagcgcaa
     5641 tacacatcaa aaatccatga atttaatcgt gatttttggt gcactgcaaa aaataattag
     5701 ataagatatt gacaaacaat aaagataagg tctactatta tattgtgcag tgcaacaata
     5761 tggagaatat tatgactcaa tacaattttg aaagatggag tgaaatggca aaaaaaatac
     5821 aagagccttt ccaggccatt gctgaactga atgttaaaac cctgcaaggg ttaagttatt
     5881 tgaagcctga tgaactggct caagttaaaa agcccgaaga aatgctggaa aaacaaatta
     5941 atttggcagt ggaaaatggc cacaaggctt tggattatat gcaaaaatca tttcaaatta
     6001 ttgaaaaagc tatgttaagt tttgtacaag aagctaaaaa aacaactgct gaa
//
LOCUS       DASLOE010000166         6047 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.167, whole genome shotgun sequence.
ACCESSION   DASLOE010000166 DASLOE010000000
VERSION     DASLOE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.167"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            28..1101
                     /gene="ribD"
                     /locus_tag="U6309_002485"
     CDS             28..1101
                     /gene="ribD"
                     /locus_tag="U6309_002485"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEN5505914.1"
                     /translation="MHEQFLLAALKQAKLGRGFCAPNPSVGAVAVQNGNIIAQAWHRG
                     AGTPHAEQLLLSQIPPQTPGVTLYVTLEPCNHWGKTPPCVDAIINHGIEEVVFSYFDP
                     NPIVAKSNSSEKLREHGIQVRHIPIKAIDVFYKSYAYWTMTHKPRVTVKIAQTFDGKI
                     GKSEGARVVLSNALCEQFTHQMRAASDVILTSAKTVQMDNPRMNARLDSGLVAKPVAI
                     VDRNLSLSDDTIIFSTAKHCHIYHQPQTNQAKTYPNSTHYAMPLQNGLMDLEAIITHL
                     GQLGYHDVWVEAGGAIFSALHSKGLVHRTYLYLVPGSLGHQAISAYQQQGIFEKAHTV
                     SWQAMGDNMIACLDWQEDECLPD"
     gene            1086..1700
                     /locus_tag="U6309_002486"
     CDS             1086..1700
                     /locus_tag="U6309_002486"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEN5505915.1"
                     /translation="MFTGLIENTGKVLVNRQGENSSRLVVSSSYQDLKPGESIAVNGV
                     CLTLLPADFPELCFDISPETLHVTTLSKLSSGDMVNLERAMLASTRYGGHYVSGHVDT
                     RAKVKSIHAVNEFIAMDLEGFIPEHNHYLIPKGSITVDGVSLTINTVSEQTVGLMLVP
                     HTLSMTNLGSLRVGQQVNIEFDYLTRIVAHQLQIIGKLNQEEVS"
     gene            1697..2905
                     /locus_tag="U6309_002487"
     CDS             1697..2905
                     /locus_tag="U6309_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEN5505916.1"
                     /translation="MKHSLATIEEAVATLKAGKMIILMDDEDRENEGDLVIAAEHATP
                     EAINFMSRLGCGLICLPMAEELIDKLQLPMMARHNKSPYGTAFTVSIEAANGVSTGIS
                     ARDRARTIQVAIDPLSGPSDIISPGHVFPLRARKRGVLERPGQTEGSVDLVRLAGLTP
                     AAVICEIINEDGTMSRRDELALFSKKHQIPLVTIKDLINYRIRHENLINPVATTQIPL
                     EENGVFTMTVFENQIDSGEHFVLVKPPLYGNQVPLVRIHSECITGDVFGSRKCDCGKQ
                     LELSLSQIGAEGGILIYLRQEGRGIGLANKLKAYALQEQGFDTVDANLELGLPADDRD
                     YAVAYQILKYLGVEALRLLTNNPLKIASLERYGMKITQRIPLEIKPSRENHCYLKTKK
                     IKLGHLLAID"
     gene            2913..3380
                     /locus_tag="U6309_002488"
     CDS             2913..3380
                     /locus_tag="U6309_002488"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEN5505917.1"
                     /translation="MREIKTIVSDGVTFPIALVVSTFNELITSALKKGALDRLTELGF
                     KSEDITLVEVPGAVEIPFVAQLLAKKQKVEVIVALGAVIRGETSHYDYVCDQVSQGCQ
                     RVMLDYNIPVIFGVLTTENDEQALARVGGTHGHKGRDAIDCAVSMRSIKQQLQ"
     gene            3506..5143
                     /locus_tag="U6309_002489"
     CDS             3506..5143
                     /locus_tag="U6309_002489"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEN5505918.1"
                     /translation="MTKYIFITGGVVSSLGKGIAAASLAAILEARGLRVTLIKLDPYI
                     NVDPGTMSPFQHGEVFVTNDGAETDLDLGHYERFVKTTMTKRNNFTSGKIYENVIKKE
                     RRGDYLGGTVQVIPHITNEIKRCIKLGADAFDVAMVEIGGTVGDIESLPFLEAIRQMR
                     IELGSQRAIFIHLTLVPYIATSGETKTKPTQHSVKELRSIGIQPDVLICRSEKPLSMA
                     DRAKIALFTNVEKEAVISLEDANSIYQIPMILHAQHLDEIVVKKLSLEAKQADLSEWQ
                     RVVDMQAVQTMTVKIAMVGKYTELNDAYKSINEALLHAGIHTETKVEIIYFDAEMIEK
                     HGALLLESIDAILVPGGFGERGVEGKIKAIQYAREHKVPFLGICLGMQTAVIEFARNV
                     VGLTGANSTEFNKETLYPVLGLISEWMDADGSKQIRDENTDLGGTMRLGGQYCHLAEG
                     TLARKIYGKPQIIERHRHRYEVNNKYVDSLVKHGLIISGRSADNSLVEMIELADHPWF
                     LACQFHPEFTSNPRDSHPLFKEFVLAARIHHQEKDKK"
     gene            5140..5964
                     /gene="kdsA"
                     /locus_tag="U6309_002490"
     CDS             5140..5964
                     /gene="kdsA"
                     /locus_tag="U6309_002490"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEN5505919.1"
                     /translation="MRLCGFEAGLDKPLFLIAGPCVIESEELALETAGYLKEMCSQLN
                     IPFIYKSSFDKANRSSISSYRGPGFEKGLSILEKVKSQIGVPVLTDVHEDTPLFEVSS
                     VVDVLQTPAFLCRQTNFIQKVAAMNKPVNIKKGQFLAPWEMKHVIAKAKAQGNEQIMA
                     CERGVSFGYNNLVSDMRSLVIMRETGCPVVYDATHSVQLPGGNNGVSGGQREFIPALA
                     RAAVAVGISGLFMETHPDPDKALSDGPNSWPLDKMKQLLESLKAADEVYKKYSTDF"
BASE COUNT         1826 a         1038 c         1390 g         1793 t
ORIGIN      
        1 cgaccacatt aagaaggtgg ttaatatatg cacgaacagt ttctgcttgc agcacttaaa
       61 caagccaaac tggggagagg cttttgtgcc cccaatccca gcgttggggc ggttgctgtg
      121 caaaatggta atattatagc acaggcatgg catagaggtg caggtacacc gcatgcagaa
      181 caattattgt tatcacaaat tccaccccaa acgccagggg ttacgcttta tgtcacgctg
      241 gagccttgta atcactgggg caaaaccccc ccttgtgttg atgctattat caatcatggt
      301 atagaggagg ttgttttttc ctattttgac ccaaacccta ttgttgccaa aagtaactcc
      361 tcagaaaaat tacgtgaaca tggaatacaa gtcaggcata ttcctataaa ggcaattgat
      421 gtgttctata aaagctatgc ttattggact atgacccata aaccaagagt cactgttaaa
      481 atagcacaaa cttttgatgg taaaattgga aagtctgagg gagctcgagt ggttttgtcc
      541 aatgctttat gcgaacagtt cactcatcaa atgcgcgctg ccagtgatgt catattaacc
      601 agtgccaaga cagttcaaat ggataatcca agaatgaacg ccagattaga tagtggattg
      661 gtagcaaagc cagttgccat tgttgaccgc aatttaagtt tgtctgatga cacaatcatt
      721 ttttcgacag caaaacattg ccacatttat catcaaccac aaacaaatca ggcaaagact
      781 tatcctaata gcactcatta tgcgatgcct ttgcaaaatg ggttgatgga cttggaggca
      841 ataataactc atcttggcca actgggatat catgatgtat gggttgaagc gggcggagct
      901 attttttctg cgttacatag taagggattg gtgcatagaa cctatttgta tttagttccc
      961 ggtagtttag ggcatcaagc gatttcagct tatcaacaac aggggatttt tgaaaaagct
     1021 catacggtga gttggcaagc tatgggtgac aatatgatag catgtttgga ttggcaggag
     1081 gatgagtgtt taccggattg atagaaaata caggcaaagt attagtgaat aggcaaggag
     1141 aaaattctag tcgtttagtc gtttcttcaa gctatcagga tttgaaacct ggcgagagta
     1201 tcgctgtaaa tggggtttgt ttaacccttt tgccagcaga ttttcctgaa ctgtgttttg
     1261 atatttcacc tgaaacgctg catgttacca cattgtcaaa attatcatct ggcgacatgg
     1321 ttaatcttga aagagctatg ctggcatcaa cccgctatgg tggtcattat gtatcgggtc
     1381 atgtagacac cagggctaaa gtgaaatcca ttcatgctgt taatgaattt atcgcgatgg
     1441 atttggaggg ttttatccca gaacacaatc attatttaat acctaaaggt agcattacag
     1501 tagatggagt tagcttgact attaatactg tatcagaaca aaccgtcgga ctcatgctgg
     1561 tgccacatac attatcaatg accaatctcg gttcattaag agtgggccag caagtcaata
     1621 ttgaatttga ttatcttacc cgtattgtgg ctcatcagct tcaaattatc gggaaattga
     1681 accaagagga agtgtcatga aacattcatt agcaacaata gaagaggctg tggcgacact
     1741 taaagccggt aaaatgatta ttctaatgga tgatgaggat agagagaatg aaggtgattt
     1801 ggtgattgct gctgagcacg ctactcctga agccattaat tttatgtctc gtcttggttg
     1861 tggtttaatt tgtttgccta tggctgaaga attgatagac aaactccaac tgcccatgat
     1921 ggctcggcat aacaaatcgc cgtatggaac ggcgtttacg gtttcgattg aagcagctaa
     1981 cggtgtctcc acaggcatct cagcaagaga cagagctcga acaattcaag tggctattga
     2041 tccactaagt gggccatctg atatcatttc acctggacat gtttttcctt tacgggctag
     2101 aaagagagga gttttagaaa gaccaggtca aacagaaggt agtgttgatt tggtaagact
     2161 tgccggattg accccggcgg cagtgatttg tgagatcatt aatgaagatg gaacaatgag
     2221 cagacgtgat gaattagctc ttttttcaaa aaaacatcaa atacctttag tcaccattaa
     2281 agatttgatt aattaccgaa ttcgtcacga aaatcttatt aatcctgtcg caactactca
     2341 gattcctctg gaggagaatg gtgtttttac tatgactgtt tttgagaatc agatagattc
     2401 tggtgaacat tttgtcttgg ttaaaccccc tttgtatggt aatcaagtgc ctttggttcg
     2461 aattcattct gagtgcataa caggtgatgt ttttggctcc agaaaatgtg attgtggaaa
     2521 gcaactggag ttgtctttat cccagatcgg agctgaaggt ggtatattaa tttatttgcg
     2581 tcaggaaggc cgtggaatag gtttggcaaa caaattaaag gcgtatgctt tgcaagagca
     2641 ggggtttgat acagtcgatg ctaatctgga attaggattg cccgctgacg atagagatta
     2701 tgccgtagca taccaaatat tgaaatattt gggggttgaa gcattaaggt tgctgacaaa
     2761 taatccttta aaaatagctt ctcttgagcg ttacggaatg aaaattactc aacgtattcc
     2821 tttggaaatt aagccctcca gagaaaatca ctgttattta aaaaccaaaa aaataaagct
     2881 ggggcattta ttagccattg attaggataa acatgagaga gataaaaaca attgttagtg
     2941 atggtgtcac gtttccaata gcgctagttg taagtacttt taatgaattg ataacctctg
     3001 cattaaaaaa aggggcgtta gacagattga ctgaactggg ttttaaatcg gaggacatta
     3061 ccttagttga ggttcctggc gcagtagaga ttccgtttgt tgcacagttg ctagcgaaaa
     3121 aacaaaaagt agaagtcata gtggcacttg gagcagttat ccgtggtgaa acctctcatt
     3181 atgattacgt ttgtgatcag gtcagtcagg gatgccaaag ggttatgtta gattataata
     3241 tcccggttat ctttggtgta ttgactactg aaaatgatga gcaggctttg gcaagagtag
     3301 gaggaactca cggccataaa gggagagatg cgatagattg tgcggtttcc atgcgttcta
     3361 ttaaacaaca attacaatag ttctattaat tttgctcaaa tttctgttat ttatattttc
     3421 attttgatag aattgaatcc cgtttacatg caaattaaat tgaacaccaa attggtggat
     3481 tcggcatttt gagcggggtc aaaaaatgac aaaatatatt tttattactg gcggggttgt
     3541 gtcttcttta ggaaaaggca tagcagccgc atctcttgca gctattcttg aagctcgtgg
     3601 tcttcgcgtt acgttgataa agcttgatcc ctacattaac gttgatcctg gtactatgag
     3661 tccctttcag catggtgagg tttttgtaac gaatgacggg gcggagaccg atttggattt
     3721 gggacattat gagcgctttg ttaaaacaac aatgaccaag cgtaataatt tcacatcggg
     3781 aaaaatttac gaaaatgtaa ttaagaaaga aagacggggt gattatttag gagggacagt
     3841 tcaggttatt cctcatatta ccaatgaaat caaacgctgt atcaaacttg gtgcggatgc
     3901 attcgatgtg gctatggttg aaataggagg aactgttggt gatattgaat cattaccctt
     3961 tcttgaagca attaggcaga tgcgtattga gttaggttca caacgagcaa tatttattca
     4021 tcttactttg gttccttata ttgctacttc tggggaaacc aaaaccaaac caactcaaca
     4081 ctcggtaaag gaattgcgtt ctattggtat ccagccagat gtattgattt gccgttcaga
     4141 aaaaccatta tccatggcag acagggctaa aatcgcatta ttcactaatg ttgaaaaaga
     4201 agctgtgatt tctcttgagg atgcgaatag tatttatcaa atacctatga ttctacatgc
     4261 gcagcattta gacgaaattg ttgttaagaa actaagcctg gaagctaagc aggctgattt
     4321 gagcgagtgg caacgagtag ttgatatgca agcagttcaa acgatgacag tgaaaatcgc
     4381 tatggtaggt aaatatacgg agctgaatga tgcctacaag tcgattaatg aagctttatt
     4441 gcatgctgga attcatactg aaacaaaagt agaaattatc tattttgatg ctgagatgat
     4501 agaaaagcat ggcgctttat tgcttgaatc cattgatgct attttggtac ctggtggttt
     4561 tggtgagaga ggagtcgaag gcaaaattaa ggctatccaa tatgctcggg agcataaagt
     4621 tcctttttta gggatatgtt tgggtatgca aacagctgtt attgagtttg cgagaaatgt
     4681 agtcggttta acaggagcta attcgactga gttcaataaa gaaacattgt atccagtgct
     4741 tggtttaatt agtgaatgga tggatgccga tggtagcaag cagatcaggg acgaaaatac
     4801 tgacttgggt ggaaccatgc gcttgggcgg acaatactgt catcttgctg aaggcactct
     4861 ggcaagaaaa atttatggca aaccacaaat tatagagcgc catcgacatc gatatgaagt
     4921 caataacaag tatgtggata gtttagtaaa acatggctta attatttcag gacgatcagc
     4981 agataattca ctggttgaga tgatagaatt agcagaccac ccctggtttt tagcttgtca
     5041 gtttcatcct gagttcacgt caaatccaag agatagtcat cctctattta aggaatttgt
     5101 acttgcagct cgtattcacc atcaagagaa ggacaaaaaa tgagattatg tggttttgaa
     5161 gcaggcttgg ataagccatt gtttttgatt gccggccctt gtgttattga aagtgaagaa
     5221 ctggctttag agaccgcagg atatttaaag gaaatgtgca gtcaattgaa tattcctttc
     5281 atctataagt cttcttttga taaagcaaac cgttcatcta tttccagtta cagggggcct
     5341 ggctttgaaa aagggttatc gattctagaa aaggtcaaat cacaaatagg tgttcctgtt
     5401 ttgactgatg ttcatgaaga cacgccctta tttgaagtct ccagtgtggt agatgtatta
     5461 caaacgccag ccttcctatg cagacaaacc aattttatcc aaaaagttgc tgccatgaat
     5521 aagccggtta atattaaaaa gggacagttt cttgctccct gggaaatgaa acatgttatt
     5581 gcgaaagcaa aggcgcaagg taatgagcaa attatggctt gtgagcgagg agtgagcttc
     5641 ggctacaata atttggtttc tgatatgcgt tccctcgtta tcatgcgtga aaccggctgt
     5701 ccagtggttt atgatgcaac acattcggtt caactgcccg ggggcaataa tggtgtatcc
     5761 ggggggcaga gagaatttat tccggctctt gcaagagcag ctgtagctgt tggaatatca
     5821 gggttattta tggaaactca ccctgatccg gataaagcac taagtgatgg tcccaatagt
     5881 tggcctctag ataaaatgaa acaacttttg gagtcattga aagcagcgga tgaagtgtat
     5941 aaaaagtaca gtactgattt ctaaattaaa tgatatcccg tgtaaaaaga agtatggcac
     6001 gggaatattt tttatttttc tcctcgcttt tatctgatta atcatac
//
LOCUS       DASLOE010000167         6031 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.168, whole genome shotgun sequence.
ACCESSION   DASLOE010000167 DASLOE010000000
VERSION     DASLOE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.168"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..119
                     /locus_tag="U6309_002491"
     CDS             <1..119
                     /locus_tag="U6309_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEN5505920.1"
                     /translation="TQENGNIKFGVEAPRSINIHREEVFHAIKQKETLSTAD"
     gene            130..1020
                     /locus_tag="U6309_002492"
     CDS             130..1020
                     /locus_tag="U6309_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN5505921.1"
                     /translation="MQVIPFRYFILLAGTIVVLSLVALVINHFIYQFPGNNYFPPNTP
                     VMACIILLNYIGLILFFGKKNRVTSSGLELINFFVVMSIIALATNAVQLTPYPPIDKQ
                     IIKLEAQFGINMISILEWMNKYQHFKYLLAIIYDTLPYQMSILPLCVIVSGRFYILKD
                     YYFLLLCTTLMGFSYYYFFPTTAPASELQSSLFVPEQIATGLKFHQIHNHIQPTTIEG
                     GLIALPSFHTIWAILCVYLIKDWFIPCLLLSIINVLLILSCVLLGWHYPIDILAAIIL
                     VGLSYYLLTVIKVEARYLDL"
     gene            complement(1053..1787)
                     /locus_tag="U6309_002493"
     CDS             complement(1053..1787)
                     /locus_tag="U6309_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEN5505922.1"
                     /translation="MEQKSKSIIRVWYLILLLSQYSFANVYVLPEAGNTIGEIQVAFS
                     EYGETIDEVGRRFNVGYYEMVRANPGVDARYPLAANTKLIIPSRFILPNVPRKGVVIN
                     LAEYRLYYFPPDDNVVMTYPVGIGKKGWETPLGLSRITSKEINPTWRPTVKLQKAAES
                     IGAPIPSEFPPGPHNPLGQHVLRLGWPAILIHGSNRVDGIGSKVSAGCIRMLPEDIEH
                     LYSLVSVGTLVRIINAPLQRQADFKQ"
     gene            complement(1847..3865)
                     /locus_tag="U6309_002494"
     CDS             complement(1847..3865)
                     /locus_tag="U6309_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEN5505923.1"
                     /translation="MNNYKHWDLQRDKDAILWLGLNRRDAAVNSINEEVLDELNSLLH
                     EISHDKKAMGLIIYSLKEKGFIAGADVNAFSQFESSSQAVDFLQKGQTVFARLQALSI
                     PSVAMIDGFCMGGGYELALACTYRIATDDKDTRIGLPEVMLGIHPGWGGTVRLPQLIG
                     GFKALSQVILTGSAIPAAKAKSLGMIDEVVPVRQLRRAAVYFIKNKPPRHRASFIESL
                     TNSSLLRKAIAYLLRYNVAKRVRKEHYPAPYAVIDLWEKEGGVGERAYLKEVDSIEKL
                     VSQNDTAKNLIRAFLLRERLKGFAKDSHFKAAHVHVIGAGVMGGDIAAWCALRGLQVT
                     LQDKSYEQIAPAIGRAHKLYSKKLRKPRLIQAAMDRLIADPEGHGVKRADVIIEAVFE
                     NLAVKQEIMKTVEKNAKKDAIIATNTSSIPLDEISSVMSKPERLVGIHFFNPVAKMDL
                     VEIVSSSSTAKKIQQSSCAFVNQIGKLPLPVKSSPGFLINRVLMPYLMECVQLLDEGY
                     SAETIDEAALQFGMFMGPVELADTVGMDVCLAVAENLTSHFGGTIPQRLRDLVKEGKL
                     GRKTGEGFYKYKNGKVIKKKAPSHVDKTIADRLILRMVNESAACIREKVVEDSDLLDA
                     GMIFATGFAPFRGGPMNYANDFGKDKLETLFKTFESKYGERFKVDTSL"
     gene            complement(3858..5177)
                     /locus_tag="U6309_002495"
     CDS             complement(3858..5177)
                     /locus_tag="U6309_002495"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEN5505924.1"
                     /translation="MKHQSNLSGRNVYVVDGSRTPFLKAKGVGPFCGSDLAVAAGTTL
                     LNRQPFSPDELDEVIIGSAMPGPDEANIARVVALRLGCGDKVPAFTVMRNCASGMQAL
                     DNAAIQIATGRSNLVLAGGTDAMSHAPLLFNEKMASWLANWFAAKSMGQKLALITKFK
                     PSYLAPVIALLRGLTDPIVGLNMGQTAEKVAYRFNITREQMDEFAAQSHLRIAQAYAE
                     NRMSEVAPIIDYKGCIYPQDDGIRADSTVEKLSKLKPFFDKKYGMVTPGNSSQITDGA
                     CLLLLASAEAVKKYALPVLGRIVDSQWAALDPSQMGLGPVHAATPILERHNLKPQDLD
                     CWEINEAFAAQVLGCLAAWNDEEYCKTQLGIKEAIGSPSLDRLNRDGGAIALGHPIGA
                     SGARIVLHVLKSLEQRNESRGMAAICIGGGQGGAMYLERVTEVKGHE"
     gene            complement(5342..>6031)
                     /gene="lem11"
                     /locus_tag="U6309_002496"
     CDS             complement(5342..>6031)
                     /gene="lem11"
                     /locus_tag="U6309_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem11"
                     /protein_id="HEN5505925.1"
                     /translation="KKKRTNPEVTPFVDQKDVANPFEIYKKIGELAKIDLLKDLENRT
                     LAYLNFSVKIQTEKIADMLNDLLNRIQNQQVIVSELYQYLLPYKGKDDLFDALIFSLK
                     RELKKLSHNFSPKTQLSSISKINLVLAPSLKLDHLPRYNPEIVNICEKLENYVFRGSK
                     KKSSFFSAKNHCGYTKEVNEIVQRILRGDLLTAEAIFHELAKINIESRKDELILLRND
                     LQSEKVCVRKL"
BASE COUNT         1751 a         1328 c         1011 g         1941 t
ORIGIN      
        1 aaactcagga aaatggaaat ataaaatttg gtgtggaggc acccaggtca atcaatattc
       61 acagagaaga ggttttccat gcaattaagc aaaaagaaac tcttagcaca gccgactaac
      121 aacaaataaa tgcaagttat acctttcaga tactttattt tattggctgg aactatagta
      181 gttctatcgc tagtagcttt agttatcaat cattttatct atcaattccc cgggaataat
      241 tattttccac ctaacactcc agtcatggct tgcattatct tgttaaatta tattggattg
      301 attttatttt tcggtaaaaa aaatagagtg actagctctg gattagaatt gatcaacttt
      361 tttgttgtga tgtcaatcat tgctttagcg acaaacgctg ttcaattaac gccctatcct
      421 cctatagaca aacaaatcat taaactggag gcgcaattcg gcattaatat gatatctatt
      481 ctggagtgga tgaataaata tcaacatttt aaatacttat tagcgattat atatgatact
      541 ctaccatatc aaatgagtat cttaccccta tgcgtaattg tatcagggcg attttatatc
      601 cttaaagatt attatttcct attactctgc acaacattaa tgggtttttc ttattactac
      661 tttttcccaa ccaccgcacc tgcaagtgaa ctacaaagca gtttatttgt ccctgaacaa
      721 atagctacag gacttaaatt tcaccaaatt cataatcata tacaacccac aaccattgaa
      781 ggtggtttaa tagcccttcc ctcatttcat acaatctggg caattttgtg tgtctactta
      841 atcaaagact ggtttatacc ctgtctattg ctgtcaatca ttaatgttct attaatcctt
      901 tcatgtgtac tgctgggatg gcattacccg attgacattt tggcggccat aatacttgtt
      961 ggattatctt attatctgtt aactgtaatt aaagttgagg cccgttattt ggatttataa
     1021 gcattaacct gaaaatctat atctgaaaat agttattgct taaaatcagc ttgcctttgt
     1081 agaggagcat ttataattct tactagcgtc cctacagaca ctaaactata taggtgctca
     1141 atatcctcag gaagcatacg aatacatccc gcactgactt tggacccaat cccatcaact
     1201 cgattagaac catgtatcaa gatagctggc caccctaaac gcaatacatg ttgccctaaa
     1261 ggattgtgag ggccaggggg aaactcactg ggaataggag caccaatgct ttcagctgcc
     1321 ttttgtaatt ttactgtagg tcgccaggta ggattaattt ccttggaggt gattctgctt
     1381 aatcccaaag gagtctccca tcctttttta ccaatcccaa caggataagt catgacaaca
     1441 ttgtcatctg gaggaaaata atataatcga tattctgcta aattaatcac aacacctttt
     1501 ctgggcacgt tggggaggat aaaacgagat ggaataatta attttgtgtt agcggctagc
     1561 ggatatctgg catccactcc cgggttagca cgtaccattt cataataacc gacattaaaa
     1621 cgccggccga cctcatctat agtttctcca tactctgaaa atgcgacctg aatttcaccg
     1681 atggtatttc ccgcttcagg caatacataa acattcgcaa aagaatattg actgagtaaa
     1741 agtattaaat accaaactct tattatagat tttgattttt gctccatatg atgcttttct
     1801 ctctaaaaag caaggtaaaa ccataacgtt ttaccttgca gagaaattat aagctggtat
     1861 ctaccttaaa acgttcgccg tatttagatt caaatgtttt aaataaagtt tccagtttat
     1921 ctttaccaaa atcatttgcg taattcatag gccctccacg aaatggagca aaacctgtgg
     1981 caaagatcat tccagcatcc aacaagtcag aatcttcaac cactttctca cgtatacaag
     2041 ctgcagattc attcaccatg cgtaaaatta gtctgtctgc aatggtttta tcgacatgtg
     2101 acggggcttt tttcttaatt acttttccat ttttatattt ataaaaacct tctccagttt
     2161 ttctgcctag cttaccttct tttactaaat cacgtaatct ttgtggaata gtaccaccaa
     2221 agtgactggt cagattttcg gccacagcaa gacaaacatc cattcctact gtatcagcca
     2281 gttcaacagg gcccataaac ataccaaact gcaatgcggc ctcatctatt gtttccgcac
     2341 tatatccttc atccaataac tgtacgcact ccatcaggta aggcatgagc actctgttta
     2401 ttaaaaaccc agggcttgat ttgacaggca aaggcaattt gccaatttga ttgacgaacg
     2461 cgcaagagct ctgctgaatt ttttttgcag tagatgagct gctgactatt tctaccaaat
     2521 ccattttagc tacaggatta aagaaatgaa tgcctaccaa gcgttctggt ttactcatta
     2581 cactactgat ttcatcaagt gggatactag aggtgtttgt cgctatgatc gcatcctttt
     2641 tggcattttt ttccacagtt ttcattattt cctgtttaac agctaaattc tcgaaaacag
     2701 cctcaataat gacatctgct ctttttaccc catgcccttc ggggtctgcg attaatctat
     2761 ccattgctgc ctggatcaat cgcggcttac gcaatttttt actataaagt ttatgtgctc
     2821 tccctattgc aggggcaatt tgttcataag atttatcctg taatgtgacc tgaagacctc
     2881 gcaaagcaca ccaggccgcg atatcccctc ccataactcc agctccaatc acatgaacat
     2941 gagccgcttt gaagtggctg tctttggcaa acccttttaa tcgctcacgc aataaaaatg
     3001 ctctgataag attcttggcg gtatcatttt gggatacaag tttttctata gagtccactt
     3061 ctttaagata agccctctct cccactcccc cttctttttc ccacaggtca atcacggcat
     3121 atggtgcagg ataatgttct tttcgaactc gcttggctac gttataacgc agcaaataag
     3181 caatagcttt tctaagcaga gagctattcg ttaaactttc aataaatgat gccctgtgtc
     3241 gtggtggttt attctttatg aaataaacag cagctcttct tagctgacga acaggtacta
     3301 cctcatcaat cataccgagg cttttagctt ttgcagcagg tattgcacta ccggttaata
     3361 tgacttgtga taaagcttta aaaccaccta ttaactgagg caaacgcaca gtaccgcccc
     3421 accctggatg aatacccagc attacttcag gcaaaccaat gcgtgtgtct ttgtcatccg
     3481 ttgcgattcg gtaggtacag gctaaagcta actcataacc accgcccatg caaaaaccat
     3541 cgatcattgc gacacttgga attgataagg cttgcaaccg ggcaaataca gtttgtcctt
     3601 tctgcaaaaa atcaactgct tgtgaggagc tctcaaattg tgaaaaagcg ttaacatcag
     3661 cacccgcaat aaaccctttt tccttgagcg aataaataat taatcccatt gcttttttat
     3721 catgcgatat ttcatgcaac aagctgttta actcatccag gacttcttca ttaatgctat
     3781 taacagcagc atctcttcta tttaatccaa gccacagaat ggcgtcttta tctcgttgta
     3841 aatcccaatg tttataatta ttcatgtcct ttcacctcag taactcgttc aagatacata
     3901 gctcctccct gaccacctcc gatacaaatt gctgccatac ctcgcgactc atttctttgt
     3961 tctaaagatt taagaacgtg caacacaata cgagccccac tggcaccaat aggatgacct
     4021 aacgcaattg ctcctccatc tcgattgagc cgatccaatg atggcgatcc aattgcctcc
     4081 ttaatgccaa gctgtgtttt gcagtactct tcatcgttcc atgcagccaa acaccctaag
     4141 acttgagcgg caaaggcctc attgatttcc caacaatcca aatcttgtgg ctttaaatta
     4201 tgtctttcta atataggggt tgccgcgtga acaggaccca aacccatttg agagggatct
     4261 agagcagccc attgtgaatc aactattcga cctaataccg gtaaagcata tttttttaca
     4321 gcctcagcgc tggccaatag caacaaacag gcaccatcag ttatctgtga actgttacct
     4381 ggagtgacca tgccatattt tttgtcaaaa aaaggtttta attttgacaa tttttccacc
     4441 gtggaatctg ccctaatccc atcatcttga ggatatatac aacctttgta atctataatt
     4501 ggggcaactt cagacattct attttcagca taagcttgag ctattcttaa atggctttga
     4561 gctgcgaatt catccatttg ctctcttgtg atgttaaatc gataagctac tttctcagct
     4621 gtttgtccca tattcaatcc tactatagga tcagtcaggc ctctgagcaa agcgattacc
     4681 ggtgccagat aagaaggttt aaactttgta attaaagcca gcttttgccc catactttta
     4741 gctgcaaacc agtttgccag ccaggaagcc attttttcat taaataacaa cggtgcatga
     4801 ctcatggcat ctgttccacc agcgaggacc aaattactgc gccctgtggc aatttggatt
     4861 gcggcattat ccaatgcctg catgcctgac gcacaatttc tcattacagt aaatgcaggc
     4921 accttatcac cacatcccaa acgcagagca accacgcgag cgatattggc ctcgtctggt
     4981 ccaggcatag cgctaccaat aatgacctca tcaagctcgt ccggagaaaa tggttgacga
     5041 tttagcaaag ttgttcctgc agcaactgcc aaatctgaac cacagaatgg acctactcct
     5101 tttgctttaa gaaaaggcgt tcgacttcca tcgacaacat aaacgttacg accacttaaa
     5161 ttcgactggt gtttcattag tcctccttgt tagggcctct gcataagaat ctatactaaa
     5221 atcaacagat tcaaagatta gcagtattct tgatattttg gaaatcttaa gatgattttt
     5281 ttgatgacat taaattacgt aaatcattcc tggaagtttt tattctttaa gccttattct
     5341 gttaaagctt tcttacgcat accttttcac tttgcaaatc atttctaagc aaaatcaatt
     5401 catctttacg actctctata ttaattttag ctaattcatg aaaaattgct tctgctgtta
     5461 aaagatctcc cctcaatatt ctttgcacta tttcatttac ttctttggtg tagccacaat
     5521 gattttttgc tgaaaaaaat gaacttttct ttttggatcc tctaaagaca tagttttcca
     5581 atttttcaca aatattcact atttctggat tgtacctggg cagatggtca agttttaaac
     5641 ttggagcaag cacgagattt atctttgata tgctggaaag ttgtgtttta ggagagaaat
     5701 tatgacttaa tttttttaat tctcttttaa gtgaaaatat caaggcatcg aacaaatcat
     5761 ctttgccttt ataaggtaga agatattggt acagttcaga gactataact tgctgatttt
     5821 gtatacgatt taataaatca tttagcatgt cggctatttt ttctgtctgg atttttactg
     5881 agaaattcag ataggcaagt gtacggtttt ctaaatcctt cagtagatct atcttggcta
     5941 attcacctat ctttttgtat atttcgaaag gatttgctac atctttttga tcaacaaatg
     6001 gagtgacctc cgggtttgtt ctttttttct t
//
LOCUS       DASLOE010000168         5969 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.169, whole genome shotgun sequence.
ACCESSION   DASLOE010000168 DASLOE010000000
VERSION     DASLOE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.169"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..359)
                     /gene="ravI"
                     /locus_tag="U6309_002497"
     CDS             complement(<1..359)
                     /gene="ravI"
                     /locus_tag="U6309_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavI"
                     /protein_id="HEN5505926.1"
                     /translation="MTRLEELLYTLTSVIIRYHDSQPKVLNKLIVESNPILLRRKALA
                     LAKEIIQDKDIECKGRLEELIQNCTKGYPDRKPFLYFILNEVLFLKSMLDRKSSFEPS
                     KLEGYKKQIAQLLIDLK"
     gene            776..1840
                     /locus_tag="U6309_002498"
     CDS             776..1840
                     /locus_tag="U6309_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEN5505927.1"
                     /translation="MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEI
                     PASALEGNMIKDVDVVANCIKKLVERLKFTSKNVALAVPDSAVISKVVQINEGLTDLE
                     MEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
                     AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFS
                     REEKFGGMQLVEAIAEHYHMNLEQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTL
                     QFFYSTSQHGFVDHILLAGGMARQPGLASLIQEQLGVTTTVANPLSHMSFGKKVNLDV
                     INSDAPTLMVACGLALRHVE"
     gene            1844..2392
                     /locus_tag="U6309_002499"
     CDS             1844..2392
                     /locus_tag="U6309_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEN5505928.1"
                     /translation="MTEINLLPWREQKREQEKKLFTTMLLIGIIIAAAIVFLMNSYVS
                     HLISNQTNRNQILQKEITALDEQIKEIKALKQIREGLISRMSIVQNLQSTRTLMVHLF
                     DELIKITPPGVYITKMQREHDVVTLWGYSESNTSISILMRNIEANVWIQNPVLTEIKK
                     NVEKKQSPNNEFKLSFVLKPKY"
     gene            2406..3002
                     /locus_tag="U6309_002500"
     CDS             2406..3002
                     /locus_tag="U6309_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEN5505929.1"
                     /translation="MDTVNLNELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKP
                     NFEQLEIQEAQEIALKTEFEKKQHQAANLQAYRNQLQLMNERFGTMLKQLPAKNEMPG
                     LLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
                     IVTLHEFSVEGVSGDGKTVSQDELVMAITAKIYRYRAQ"
     gene            2999..3583
                     /locus_tag="U6309_002501"
     CDS             2999..3583
                     /locus_tag="U6309_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEN5505930.1"
                     /translation="MIIKNNQTLILGFFGNSLCTLLTSENFRKSLLTALLVLLLSACS
                     GDNSDLIKYINEVKSRPGRPIEPIPKFAPLPIFRFPENDDRRNPFKPIDQKKRNDIYA
                     PDKKRPKQPLESFPLDALKFVGTLKKDNEIWALIKQPDGQISRVRVGDYMGQNYGRII
                     LIKNDLIKLEETVQKSGTWEKQSTTINLDTGKQE"
     gene            3587..5683
                     /locus_tag="U6309_002502"
     CDS             3587..5683
                     /locus_tag="U6309_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEN5505931.1"
                     /translation="MRKIIAILILISASLSLAYSKDNSLISVKVLPLPENRVRIDFQF
                     SNPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKTKTIQLGSLHSYNIVAVGDRVRA
                     ILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
                     EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITM
                     QQEGKKVRMTILSKGDYGHFVYQVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLN
                     FQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQALDIILTTQGLDKRRK
                     GNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSL
                     LSDRGKISVDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQD
                     IGIRWGVSRPTHLSGTLEGANELARGTAPANVVPLERRLNLDLAAAPLTGATPASVGI
                     ALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQEATSSGA
                     TAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVN
                     NGQTIVLGGIYKQDKNKVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITN
                     ALSITTIEGREKDVYK"
BASE COUNT         1993 a          948 c         1258 g         1770 t
ORIGIN      
        1 tgtttgagat caattaaaag ctgagcgatc tgttttttat atccttccag tttagatggc
       61 tcaaatgaac ttttcctatc cagcatggat tttagaaaaa gaacttcatt taaaataaaa
      121 tataagaatg gttttctgtc agggtaacct tttgtgcaat tttgtattaa ttcctcaagg
      181 cgtcccttac attcaatgtc tttatcctga ataatttctt tagccaaagc aagtgctttt
      241 cttcttaaga gaatgggatt gctttcaact attaatttat ttaatacctt aggctggctg
      301 tcatgatacc gaatgattac agaagtcagt gtatagagta gctcttctaa tctagtcata
      361 tgtaaagctc ctttttataa agctaaaaat ctaaatgtgg aaatccccta caacttggta
      421 tttcactata ttgaatgttt tgcataagtg caagtgcttg ttaactggta cattatttga
      481 aaaaaacata caagtagagg ggttgtttta cagttttttg aaagaatata aaatctgatt
      541 tgattgtaat actatgattg atatcatgaa atttgcttgg gcatcgacag aagacagaaa
      601 tggaaatgca tggatcaatt gtcagattga ttataaacgc ttaacccaaa gtaataagta
      661 aagtgattcc aatttatagt ggcttacgct aacattaatt attcaatagt taggaaaaga
      721 tctaaaaata ggagtgcttt tgggctgcaa ggaaatatac agggagatta tataagtgct
      781 caaattgctt aaacctaagc atcgttcaat tcttggcatt gacatcagtt cttccgcagt
      841 gaaaattctg gaaatatcag gttccggaga ggacatttgt gttgaaggat atggtcgtga
      901 agaaatacct gcctctgccc tagaaggaaa tatgatcaag gatgtcgatg tagtagcgaa
      961 ttgtatcaaa aaattagtag aaagattgaa gtttacatct aaaaatgtgg cattggctgt
     1021 acctgattcg gctgttatca gtaaagtagt gcaaattaat gaagggttaa ctgaccttga
     1081 aatggaagag cttgtcgtta ttgaggcgga taagtacatt ccttacccaa tagatgaaat
     1141 aaacctggat tttgaagtgt taggccattc agccaagagc tcatcactgc ttgatgtttt
     1201 aatcgtggca tcaagggcag aaaacgttaa tacccgtgtt gaagcagccc atagagcagg
     1261 gctggaaccc aaagttgttg acgttgaatc ttatgcagta gagcgttcgg tacaacaaat
     1321 tgcgcaagaa ttaccagcaa gtgggcaaga caaaattgtt gcaatagtag atattggagc
     1381 caattatact cacttatttg ttttgcatgg tatgcgacta atcttttcaa gagaagaaaa
     1441 atttggaggt atgcaacttg ttgaagctat tgcagaacac tatcatatga atttggagca
     1501 ggctattgcc ctaaaaaatc agggtaaact accggaggac tacgaatctg ctgtattaga
     1561 gccttttaag gacatgattc ttttgcagat caaacgtact ttgcagtttt tttattcaac
     1621 cagccaacat ggatttgtag accatatttt attggcggga ggaatggcaa gacaaccagg
     1681 attggcatcg cttattcaag aacagttagg agtgactact acagtggcaa atccactatc
     1741 ccatatgtct tttggtaaaa aagtgaattt ggatgtgatt aacagtgatg cgcctacact
     1801 gatggtggct tgtgggttgg ctttgaggca tgtcgagtaa aaaatgactg aaattaattt
     1861 attgccatgg cgtgaacaaa aacgtgagca ggaaaagaaa ttatttacaa ccatgctatt
     1921 gatagggatt atcatcgctg cagcaattgt ttttttaatg aactcttatg tctctcattt
     1981 aataagtaat caaacgaacc gaaatcagat tttacaaaaa gaaattacag ctttagatga
     2041 acagatcaag gagattaaag cattaaaaca aatcagggaa ggattaattt caagaatgtc
     2101 gattgtgcag aatctgcagt caacaagaac tttaatggtt cacttatttg atgagttaat
     2161 aaaaattact cctccaggag tttacataac caagatgcaa agagaacacg atgtggtaac
     2221 actttggggg tattccgaat cgaatacaag tatttctatt ttgatgagaa atatagaggc
     2281 caatgtctgg atacagaatc ctgttctaac tgaaattaaa aaaaatgttg aaaaaaaaca
     2341 gtctccaaac aatgaattta aattaagttt tgtgcttaaa cccaaatatt aggctgataa
     2401 aaatcatgga cacggtcaat ttaaatgaat taactctaga gaatgttggg cagtggccaa
     2461 tgccggttaa aatcggagta attttaggca tcagtatact gataattgga ttaggatatt
     2521 ggcttattgt taagcccaat tttgagcaat tggaaataca ggaagcccag gaaattgctt
     2581 tgaaaacaga gtttgaaaaa aagcaacatc aggcagcaaa tctacaagcc tatcgtaatc
     2641 agctacagtt aatgaatgaa cgctttggga ctatgctaaa acaacttccg gcgaaaaatg
     2701 aaatgcctgg gcttttggag gaaatatcta aaacggggat cgcatcgggt ttgaaatttg
     2761 aattgtttgc accacaacct gaggtagtgc atgattttta tgtggagtta cctataaaaa
     2821 ttacgattgt cggatcctat atgcaattgg caagattttt aagtcgtgta gctgaaatga
     2881 acagaatagt gactttgcat gaatttagtg ttgagggggt gtccggggat ggtaaaacgg
     2941 tctctcagga tgaattggtg atggctatca ctgcgaaaat atacagatat cgggcacaat
     3001 gataattaaa aataaccaga ctcttatatt aggctttttc ggaaattctc tgtgcacttt
     3061 actcacctca gagaatttcc gaaaaagtct attgacggcg ttattggttc tattacttag
     3121 tgcctgctct ggtgataaca gtgaccttat taagtacatc aacgaagtga aatcaagacc
     3181 agggagacct atcgagccta ttcccaaatt cgcccctttg ccaattttta ggtttccaga
     3241 aaacgacgat aggcgaaatc cattcaagcc tattgatcaa aaaaagagaa atgatattta
     3301 tgccccagat aaaaaaaggc ctaagcagcc tttggaatct tttcctctgg atgctttaaa
     3361 atttgtaggt actttaaaaa aagacaatga aatctgggct ctgattaagc aacctgacgg
     3421 ccagattagc cgtgttaggg ttggagatta tatgggacaa aattatggac gtattatttt
     3481 gattaaaaat gatctcatta aattggaaga aactgtacag aaatcaggaa cgtgggaaaa
     3541 acagtccacc acaattaatt tggatactgg caagcaggag tgaggcgtga gaaaaattat
     3601 cgcgatttta atattaatta gtgccagttt gagtctggca tattctaaag ataattcgct
     3661 catttcagta aaagtattgc cattgccaga aaacagagta cgtattgatt ttcaattttc
     3721 caacccctta aagcagttgc ccgcaagctt tattactcaa aagccgcctc gtttggtgtt
     3781 agattttatc agcactgata tgcaaattcc ttcggatcag aaaactaaaa ctattcaatt
     3841 aggctcatta cacagttata acattgttgc tgttggtgat agagtaagag ccatactgga
     3901 actggatcgt tcggtgtcct attctggcag tacggcgggc tccgtttata gccttgtcct
     3961 gaatggtaaa agtaatgatt tatttgaaaa tagcaaggag gtattcataa cgaaccaggt
     4021 tgtgaacgcc aagcatgaaa ttaaaagaat tgattttcgt ggcatagaaa agcagggagg
     4081 tagagtttta attgatgttt ctgatacggg tattccaatt gatgtaactc aagttgggaa
     4141 agaaatagtc gttaatttcc tgaataccaa gatccctttg aatttaatga aacggtatga
     4201 tgtttctgat tttcacagcc ctgtgcagat tattacgatg caacaggaag gcaaaaaggt
     4261 tcgcatgacc atcctcagca aaggggatta tgggcatttt gtttatcagg tcaataaaca
     4321 atttatggtc gatgtctttc ctttaactgc ggaagaaatt cgtcaggcga aattgaagaa
     4381 acaggtattt accggtaaac ggatttcttt gaatttccag aatattagta ttcgagctgt
     4441 gcttcaacta ctggcagatt ttaccggaat caatatggtt gtcagtgaca aagtgcaagg
     4501 agatattacg cttagattaa atgatatccc ctgggatcag gctctggata tcattttaac
     4561 cacacaaggg ctagataagc gcagaaaggg caacgtcatg ttgatagata ccaaagcctc
     4621 catggataaa atggaggaag aacaactcaa aagtcaacag acaattgaaa aacttgagcc
     4681 tatccgctct gatttaatac agattaatta tgcaaaggca gcagatattg ctgttttgat
     4741 caaggataag caaaactccc tattatcaga cagaggaaaa ataagtgttg atgccagaac
     4801 gaatacaatc tggattcagg atactggcac caaaattgag gaagtaaggg aactgataaa
     4861 gcaattggat atccctgtta agcaagtgct tattgaggca agaattgttg aagtaactaa
     4921 agattttagc caggatattg gaatacgctg gggggtatca agaccaactc acttaagtgg
     4981 tacactggaa ggtgctaatg agttagcgag aggcacagca ccagccaatg ttgtcccctt
     5041 ggaaagaagg ttaaatcttg atttggcagc tgctccttta actggagcta caccggcctc
     5101 ggtaggtatt gcgctagcta aacttaatga taacattctg cttgatctgg agttgtctgc
     5161 tttggaaagc gaagggcgtg ctgaattaat atccagtcct cgcctgatta ctacgaatca
     5221 acaggcagca gtcatagagt ctggtgagga gattccctat caggaggcaa catcaagtgg
     5281 agcgactgct gttgcattta aaaaagcagt attgagtttg aaagttacgc cgcaaattac
     5341 gcctgatggt aaaattttga tggatttaca gataaatcag gatactcctt ccccacaaac
     5401 attccttggt gttccagcaa taataacaaa ggagattcag acgaatgttc tggttaataa
     5461 tggacaaacc atagtacttg gaggtattta taagcaagat aagaacaagg taatcaatag
     5521 aattcctttc tttggtgagt tgcctgttgt cggtatttta tttagtaata gacaaatcac
     5581 attgaaaaat gaagaattac ttatttttat tacaccaaga ataataacga atgccttatc
     5641 gattacaacg atagagggac gtgagaaaga tgtttataaa taatcaatat tggactaatt
     5701 ttatctcatt aataaagata aaaaacatta atttaataga agtgcctcaa cacggtgaac
     5761 atgttataat taaaatatag cccgctatga tgtaatggca ttttgaaaaa aattgctcat
     5821 cagcgatgga ttgggtaaag ttgaagtatc aacttcagcg atggcagaaa agtgtaataa
     5881 ataattattt tatatgggta attaatggtt atccgttgta gaatgtcaga agtattttag
     5941 atagtaattg gatgtattcc taatttgtg
//
LOCUS       DASLOE010000169         5966 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.170, whole genome shotgun sequence.
ACCESSION   DASLOE010000169 DASLOE010000000
VERSION     DASLOE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.170"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            188..2503
                     /locus_tag="U6309_002503"
     CDS             188..2503
                     /locus_tag="U6309_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEN5505932.1"
                     /translation="MKENVSHLPRELSRMVFWSLTCLKKAIAEVYGKETYERIEQIRL
                     SMQDTIGSESFSLGKALHSLQIELSKLEKKELYQIAHGFSLMMELINTCENAYRIFRI
                     NQRKEQVYSDMPQGIHYVLTAHPTEARTNEFLKLFKEIRDYLIEVLKSPYLMNESHLD
                     QMLKISLQINISYHEKPSVEDEAQYIYQYALHPLNIKLYHSFLAKGVPIQLRTWVGGD
                     KDGHSGVNEKIMLKSLELSRSFFIAYIQECLREVLEIIKLSTSLDKNKPLADQINQLI
                     KKAYGLKKIQSKEHEKISTFKNELTATQINALKFLNFEPEQFSIISSTFHLYPALVMP
                     IELREDSAVVKESLTSIKKTTITKMLEQLHSITYGTDPKNYIRGFILSMVESAEDIKN
                     GINLVKKVFKSYALPVVPLFENQVALTNADTILNNSITEDIRQKHLKQWDGNYEVMLG
                     YSDSSKENGVLPSRLMIAKSLKNIQSTLENKNLRPIFFHGSGGSIERGGGDIKEQTAS
                     WSKEIMSNYKVTVQGEMVARLFGSSSILKSQIDKFLDIYSQKGKSNDNDYPEELSLFS
                     TRVSQKYKTLIQSDWFWQIVEQASPYHFLKELKIGSRPTKRKSGPDQRKLRAIPWILC
                     WTQTRLLFPTWWGIGSTWSELNGIEKNRLKVLYKENALFAAFVKQLGVTLSKVYLPVW
                     EQYLLQLTGSIEYLRPFQSELESTILFFYQITDEKDFCFHQPWLGESIQLRSTLIHPL
                     NLIQIEAMKRKDLPLLRKTVTGISCGMLTTG"
     gene            2842..3288
                     /locus_tag="U6309_002504"
     CDS             2842..3288
                     /locus_tag="U6309_002504"
                     /inference="COORDINATES: protein motif:HMM:NF019498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEN5505933.1"
                     /translation="MHKGSELNSFGFLAVAMMLVGISTSVFATCPTNKQTISVTKANE
                     QQWKEINDHHSHPKHAIFFHDNKSGLVKFKAGYKNPPHYYENDLAGIMLSGEIDFKIN
                     GKTISLSEGDYFTIPARTCFSSFSKNGATIAMFGHKPGSYEKVKSE"
     gene            3343..3813
                     /locus_tag="U6309_002505"
     CDS             3343..3813
                     /locus_tag="U6309_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEN5505934.1"
                     /translation="MKIKNISYYVATRVVKRIQSWLGLNTLGARAIVTNTEGHVLLVK
                     HTYQPHWYLPGGGVKKGESTKAAVIRELHEEVGLVVAEQDVILFGIYHHKYLGVNDYP
                     VIYIVKNFTSHVTHSGEIEQMGWFSLDALPEMVSPGTKRRLGEYFDNNPISEKW"
     gene            complement(3864..5117)
                     /locus_tag="U6309_002506"
     CDS             complement(3864..5117)
                     /locus_tag="U6309_002506"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEN5505935.1"
                     /translation="MNTDIANQLRAAKKATTDLNLIQSDTRTIILKTLAANLEKHIEN
                     IIQENQKDLSLMLEQDPRYDRLLLNKERILSLANDVRKVASLPNPLGVNLLEKSMPNG
                     LSIKKITVPLGVIAVIYESRPNVTIDIFSLCFKSGNACILKGGKEAHFTNSYLLLLIK
                     NTLKNFNINTDIVCLLPPERALITPLLNATGLVDLCIPRGSQNLINFVRDNAKIPVIE
                     TGAGIVHTYFDKSGDLGKGKKIINNAKTRRVSVCNALDTLIIHSDRLKDLAELVEALS
                     QKNVIIYADQDAYQVLDKNYPEQLLIKAKPQDFGHEFLDYKLAIKTVPNIKAAIDHIQ
                     QFSSHHSEAVIAEDESAIDKFLTEVDAAAVYANASTSFTDGGEFGLGAEIGISTQKVH
                     ARGPMGLEALTSYKWVIRGTGQIRD"
     gene            complement(5126..>5966)
                     /gene="proB"
                     /locus_tag="U6309_002507"
     CDS             complement(5126..>5966)
                     /gene="proB"
                     /locus_tag="U6309_002507"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEN5505936.1"
                     /translation="GQPELMNLYSSLFKNENILVSQLLLTKQDFHTRQHYLNIARLLT
                     ELLKHQNIIPIVNENDSVAIEELMFTDNDELAGLIAAQINADYLILLTNVDGVFTSHP
                     NDPESKLINTIDPNSGWPNVSAVKSKLGRGGMVSKLDTARKMAGLGINTVIANVNVES
                     IVPKILNREAVGTIILASKRKSSIKRWIAFSHRTSLPSIVINDNLITLLRNGNRVMSI
                     LPIGIEDFHGECKKGDLIDILNSNNEKIGVGIARYDFLRLKDYLRLQNKPAFIHYDHL
                     HIL"
BASE COUNT         1942 a         1094 c         1052 g         1878 t
ORIGIN      
        1 aaatattttc caacacaaat taagtatatt aaaattgcat gccaaagtat tcttgaatag
       61 ttgtgccaaa atcctttgag gatcaactag agaagtagac aatgaagtat ttactgcatc
      121 agagttagat aacgaacaga cggcattcct ctacagaata aaccattcgg ataaaatgga
      181 gttttcgatg aaagaaaatg tgtcccacct accaagagag ctcagccgta tggttttctg
      241 gtcacttacc tgtttaaaga aagccattgc agaagtctat ggaaaagaaa catatgaaag
      301 aatcgagcaa attcgactat ccatgcaaga cacgatcgga agtgagtcgt tttcacttgg
      361 aaaagctctt catagtcttc aaatcgagct ttccaaactt gagaaaaaag agttatatca
      421 aatagctcat ggtttttccc ttatgatgga gctaattaat acctgtgaaa atgcctaccg
      481 catttttcgt atcaaccaaa gaaaagaaca agtttattct gatatgcctc aaggtattca
      541 ttatgtattg acagcacacc caacagaggc tcgtaccaat gaatttttaa agcttttcaa
      601 agaaattcgt gactatctta tagaagttct taaatcaccc tatctgatga atgaatctca
      661 tcttgatcaa atgcttaaaa tctcacttca aattaatatt tcctatcatg aaaaaccaag
      721 cgtggaagac gaagctcaat acatctatca gtacgcactc catccactta acatcaaact
      781 gtatcattca tttttagcaa aaggagtccc tattcaatta cgcacctggg taggaggtga
      841 taaggatggt cactctggtg ttaatgaaaa aatcatgctt aaaagcctag aactttctcg
      901 ttcttttttt attgcctaca ttcaggaatg cttaagagaa gtgctggaaa tcattaagct
      961 ttcaacttca ttagataaaa ataaaccctt agcagatcag attaatcagc ttattaaaaa
     1021 agcctatggt cttaaaaaga tacaatcaaa agaacatgaa aaaatatcga cattcaaaaa
     1081 tgaattgact gctacacaga taaatgccct aaagttttta aatttcgaac ctgagcaatt
     1141 ctccatcatc tccagtactt ttcatctgta tcccgctttg gtcatgccta tagagcttag
     1201 agaggactca gccgttgtaa aagagagttt aacaagtata aaaaaaacaa caattactaa
     1261 aatgcttgaa cagctacact ctataactta tgggactgat cctaaaaact atatacgcgg
     1321 ttttatcctt agtatggttg aatcagctga ggatataaaa aatggaatta atctggttaa
     1381 aaaagttttt aaaagctatg ccctgccagt tgtgcccttg tttgaaaatc aagttgccct
     1441 cactaacgca gatacaatat taaataattc aatcactgag gatatcagac agaagcatct
     1501 caaacagtgg gatggaaact atgaagtcat gttgggttac tccgactcgt ccaaagaaaa
     1561 tggcgtactg ccctctcggc ttatgattgc gaaaagttta aaaaacattc agtctaccct
     1621 ggaaaataaa aaccttcggc ccatcttttt tcatggttcg ggcggtagta ttgagcgagg
     1681 aggcggggat atcaaagaac aaacagcctc ctggtcgaag gaaataatgt ctaattataa
     1741 agtgactgtc cagggagaaa tggttgcaag actctttggt tcaagttcca tactcaaaag
     1801 ccagattgac aaatttcttg atatctattc gcagaaaggc aaaagcaatg acaatgatta
     1861 tccagaagag ttgtccttgt tttcgacaag agttagccag aaatataaaa cacttatcca
     1921 aagtgactgg ttttggcaaa tagtagaaca agcctcccct tatcattttc tgaaagaatt
     1981 gaaaattggc tctcgtccaa ccaaacgaaa atcaggccct gatcaacgaa agcttcgcgc
     2041 catcccatgg attctgtgct ggactcaaac acggcttctt tttccaacct ggtggggtat
     2101 aggttccact tggtcagagc taaatggcat tgaaaaaaac agacttaagg ttctttataa
     2161 agaaaatgct ctttttgctg cttttgtaaa gcaattaggt gtaaccctct ccaaggttta
     2221 tctacccgtc tgggaacagt acctgcttca attgactggc tctattgaat acctaaggcc
     2281 ttttcaatca gaactagagt caactatcct gttcttctat caaataaccg atgagaaaga
     2341 tttctgtttt catcaaccct ggcttgggga aagtattcaa ttacgatcaa cattgattca
     2401 tcctttaaat cttattcaga ttgaagccat gaaaaggaaa gacttacctc ttttaagaaa
     2461 aacagtcaca ggcatttctt gtggtatgtt aacaacagga taatagaagt aaacagcgtt
     2521 attgattcct tctataatat ttagcacagt aaagtattaa agtatgttat ttttgcttat
     2581 aggctctctt acccaaatgg caatagccgt ttggacattt tttgctgaca ccgctgaaat
     2641 gccaagagca gagataaagg agggggcgtt ggtattaact atcatactca gggacgttaa
     2701 ctgctatttt aagccattta ttggaacaag tataagtcac ctcccttcca tgaatatcga
     2761 aagaatttga tcttaattta taattgttgt aagtattgag attaatttat tgaaatttac
     2821 gataaatatt aaggagaaaa aatgcacaaa ggaagtgaat taaattcgtt tggcttttta
     2881 gccgtagcaa tgatgctggt tgggatcagt acatctgttt tcgccacatg cccaactaac
     2941 aaacaaacga ttagcgtaac aaaagcaaac gagcaacaat ggaaagaaat taacgatcat
     3001 cactctcatc ctaaacacgc tatattcttt catgataata aaagtggctt ggttaagttt
     3061 aaagcaggct ataaaaaccc cccacattat tatgagaatg atcttgcagg tataatgctt
     3121 tctggagaaa tagacttcaa aataaatggc aaaacaataa gccttagtga aggcgactac
     3181 tttactatac ccgcacgcac ttgttttagt tccttttcaa agaatggtgc tacaatagct
     3241 atgttcgggc ataaacccgg atcttacgaa aaagttaaat cagaataatt ttagattatg
     3301 ccttgattac gcagtagtga aatcaaggct ttcaattaca tcatgaagat aaaaaatatc
     3361 tcttattatg tcgctacccg cgtcgtgaaa cgaatacagt catggttggg attgaatact
     3421 ctgggtgcac gagcaatagt gacgaataca gagggacacg tgttattggt aaaacatacc
     3481 taccaacctc attggtattt accaggaggc ggagtaaaaa aaggagaatc aaccaaggct
     3541 gcggtaatcc gtgaactgca cgaagaagtt ggtcttgttg ttgcagaaca agacgtgatt
     3601 ttattcggca tttatcacca caaatatctt ggagttaatg attatccagt aatttatatt
     3661 gttaaaaact ttacaagtca tgtaactcac tcgggggaaa ttgagcagat gggatggttt
     3721 tcccttgatg cattaccgga aatggtgagc ccgggaacca aacgcagact cggtgaatat
     3781 tttgataaca atccaatatc agaaaagtgg tgattattgc agttgtcatt gttgggtggg
     3841 gtactgagaa aatatggtta tgctcaatct ctaatttgtc ccgtccctct tatcacccat
     3901 ttgtagcttg ttaaagcctc tagtcccatg ggtcctcttg catgaacttt ttgcgtacta
     3961 attccaattt cagcccctaa tccgaattcc ccgccatctg taaatgaagt agaagcgttg
     4021 gcatagacag ctgctgcatc cacttcagtt agaaatttat caattgcaga ctcatcttct
     4081 gcaataacag cctcactatg atgggaactg aattgttgaa tatgatcaat agctgctttt
     4141 atattgggta ctgtttttat tgccaattta tagtccagaa attcatggcc aaagtcttgt
     4201 ggttttgcct taattaaaag ttgttcagga taattcttat ctaaaacctg atatgcatcc
     4261 tggtcggcat agataatcac attcttttga gataaggcct ccacaagttc tgccaaatct
     4321 tttaaacgat cagaatgaat aatcaaagta tccagcgcgt tgcaaacact tactcttctg
     4381 gttttagcat tgttaataat ttttttgcct tttcccaaat ctccactttt gtcaaaatag
     4441 gtatgtacga tgcctgcccc tgtttcaatg acaggtattt tggcattgtc tctgacaaaa
     4501 tttattaaat tctggctccc tcttggaatg cataaatcaa caagacctgt ggcatttaat
     4561 aatggggtta tcaatgctct ttctggtggt aataaacaaa caatatctgt atttatatta
     4621 aaattcttta atgtattttt aattaataaa agcagataac tattagtaaa atgtgcctct
     4681 tttccacctt ttaaaatgca agcatttcca gatttaaagc atagactgaa gatatcaata
     4741 gttacattag gtcttgactc ataaatgact gcgatgacac ctaaaggtac tgtgattttc
     4801 ttaatactca aaccatttgg cattgatttt tcaaggaggt taactcctaa tgggttaggt
     4861 aaactggcta cttttctcac atcattcgcc aatgacaaaa ttctctcttt atttaacaat
     4921 aaacgatcat atctgggatc ttgctcaagc attaaactta aatctttttg attttcttgt
     4981 attatgtttt caatgtgctt ctctaaatta gccgctaatg ttttcaaaat tattgttcgg
     5041 gtatcacttt gtattaagtt aagatcagta gttgcttttt tagcggccct taattggtta
     5101 gcaatatcag tattcatttt gatacctata gaatatgcaa atgatcgtaa tgtataaatg
     5161 caggcttatt ttgaagtcta agatagtcct ttaatcttaa gaaatcatat ctggcgatcc
     5221 caactcctat cttttcatta ttggaattaa ggatatcaat taaatcacct tttttgcatt
     5281 ctccatgaaa atcttctatc cctattggta aaatgctcat tacacgatta ccatttcgta
     5341 ataaggtaat taaattatca ttaattacta tagatggtaa ggaagttcta tgactaaaag
     5401 caatccatct ttttatactg gattttcttt tactagcaag aattatggtt ccaactgctt
     5461 ccctatttag aatttttgga acaatggatt ctacatttac atttgcaatc actgtattga
     5521 tacctaaacc agccattttt ctggcagtat ccagtttgga taccatacca cctcggccta
     5581 attttgattt tacagcagag acattgggcc aacctgaatt ggggtcaatt gtgttaatga
     5641 gttttgattc aggatcattg ggatgggatg taaacacacc atcgacattg gttaagagta
     5701 ttagataatc agcattaatt tgagctgcaa taagtcctgc taattcatca ttatcagtaa
     5761 acattaattc ttcaatggct accgaatcat tttcattaac aatagggata atattttgat
     5821 gctttagtaa ttccgttaat aaacgggcaa tatttaaata atgctgtcta gtatggaagt
     5881 cttgtttggt aagcaataac tgagatacta aaatgttttc atttttaaac aagctagaat
     5941 agagattcat cagttcgggt tggcct
//
LOCUS       DASLOE010000170         5950 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.171, whole genome shotgun sequence.
ACCESSION   DASLOE010000170 DASLOE010000000
VERSION     DASLOE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.171"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            6..1283
                     /locus_tag="U6309_002508"
     CDS             6..1283
                     /locus_tag="U6309_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5505937.1"
                     /translation="MSKVEIYKRKNYLGFAWLVWGLAAAFYFSDYLARVAPGVMHRYL
                     QMDFGINEAGFGILTASFYVPYILMQIPVGLTVDRLSIRWLLTIMSLVTAFGCCVFGL
                     ADGLLTASIGRMLIGFSAAFAFICSLRLATSWFPPTMLGLLSGLTQALGMLGAAAGEA
                     PVSFLVSNVGWRHSMLIIAFLFIALSGLLYQFVQDKPGEHRNEIRSVNRISILDSLKI
                     ILSNKQTWLNAMYAGFLFGPTAVIGEAIGPAYLQFGRGLGAHAAAFATGLIFIGWGIS
                     GPLSGWISDKMGRRKPLMIISAVCGVILSSLFVFIPEMSQTTAYILFFVFGLTNTGVA
                     ISYAVSTEIHDRSVVGTSIAFTNMTSIFVGALFQPLVGRIIDMVSGPRAYNVETLLLS
                     DFQAGLKLLPLCSLVALILAFTVKETYCKPIRH"
     gene            1513..2970
                     /locus_tag="U6309_002509"
     CDS             1513..2970
                     /locus_tag="U6309_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEN5505938.1"
                     /translation="MKKLTGLVIILAVLILGGYYGMGILTERTVKKTVEVINQSNGLF
                     ADIQQYKRGWFCSDALVKWRLHVPERVVKDDDGKLQTTPAQDYQMEMPIKIYHGPIIY
                     ANKQLRFGMGFAQTVFPFPEKYNQQFNELFSQDSEKPQLDLSIFVNYLNKSTVELSIP
                     SFKLTAKDGNGKFNWMGMDSTTSMSSNLDKVEGEIEFDGAEFSKDDTKLSLGKVNSEY
                     DLHKTTTGLYLGQASFSLPSLAVVVKDQKMFEISELTLSSSSDIDDNLFSTHFSAALK
                     SLFANGQNYGPGEVEMSLRNLDAEVLAKINEQANAMQRGTDQERQQALMAMLPELPKL
                     FSKGAEFEISKLSLKLPQGMIDGNLLITLPKGDSSNPFELIQKTQGNAKLKMPIEVVK
                     QLMQQSIMQQMAKQPEMQQALIQQLQNSQGQSNQAEPTPEQIAAMQVDKQINELEQSG
                     LITVDGKDYVIEMSLSEGKFTVNGKPFDPSMMKFQ"
     gene            3160..3441
                     /locus_tag="U6309_002510"
     CDS             3160..3441
                     /locus_tag="U6309_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEN5505939.1"
                     /translation="MTTISNDAGDTTASLAESVTQSVQKYFSELKGTDPVDLYQFVLE
                     EIETPLFRAVMEHCKYNQSRAAIMLGISRGTLRTKLRRYFDDKYVGTRD"
     gene            complement(3503..4450)
                     /locus_tag="U6309_002511"
     CDS             complement(3503..4450)
                     /locus_tag="U6309_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEN5505940.1"
                     /translation="MSTMMIFTGNANPELALKISSHLQIPIGKATVGTFSDGETMVEI
                     LENVRGKDVFVLQSTCAPANNNLMELLIMADALRRSSAGRITAVVPYFGYARQDRRVR
                     SARVPITAKVVADMMASVGICRVLTVDLHADQIQGFFYMPVDNVYSTPVLLEDITKQK
                     LNNIMIVSPDVGGVVRARAVAKRLNDAELSIIDKRRSGPNKSEVMHIIGEPANKNCII
                     VDDIVDTAGTLCTAAHELKKNGAKSVRAYITHPVLSGPAVNNIKHSGLDEVVVTDTIP
                     LSAEAQNCEKIRVVSLADMLAQAIKRVNVEESVSSMFAE"
     gene            complement(4630..4704)
                     /locus_tag="U6309_002512"
     tRNA            complement(4630..4704)
                     /locus_tag="U6309_002512"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4670..4672),aa:Gln,seq:ttg)
     gene            complement(4832..5431)
                     /gene="lolB"
                     /locus_tag="U6309_002513"
     CDS             complement(4832..5431)
                     /gene="lolB"
                     /locus_tag="U6309_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEN5505941.1"
                     /translation="MNAIKRLTIILICFLTACAPPRPSEEPPANREIPLEERKAETAT
                     VSSWEIKGALAAKTKAKGWSATMNWVQQGANSYQIRLMGPLGGGAVLISKKGNTITFQ
                     DGAKITTSTNADELLLKQTGIRLPVNNLYYWVRGLPAPGGIQSEKHDQFNHLIQLKQN
                     GYTIDFTKYTSVKGIDLPSMIRLEGNGVMVKVVIKNWVV"
     gene            5494..>5950
                     /gene="coaD"
                     /locus_tag="U6309_002514"
     CDS             5494..>5950
                     /gene="coaD"
                     /locus_tag="U6309_002514"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEN5505942.1"
                     /translation="MQMVINEMKQKAIYPGTFDPVTNGHIDIITRASTIFPELIVAVA
                     SNKNKRPYLSWESRISLLEESVGHLTGVRVVGFDNLLIDFVLEQNAGIILRGLRAVSD
                     FEYEFQLAGMNRKLSKKVETLFLTPAEHLMYISSTLVREIAALNGDISQF"
BASE COUNT         1749 a         1095 c         1203 g         1903 t
ORIGIN      
        1 tgggaatatc taaagtggaa atttataaac gtaaaaatta cctgggtttt gcgtggttag
       61 tttgggggtt ggcagctgct ttttattttt ctgattattt agctagagta gcgcctggtg
      121 taatgcaccg atatctacaa atggactttg gcattaatga ggctggcttc ggcatattaa
      181 ctgcatcatt ctatgttcca tatatcttaa tgcaaattcc tgtaggattg acagttgatc
      241 gattaagcat acgatggtta ttgactatta tgtcattagt tactgctttt ggttgctgtg
      301 tatttggcct tgcggatggt ctgctgactg catcaattgg aagaatgttg attggtttca
      361 gtgctgcttt tgcattcatt tgctcacttc gccttgccac ttcatggttc cccccaacta
      421 tgcttggatt attatctgga ttaactcaag cgttgggaat gcttggtgct gccgcagggg
      481 aggcccctgt ttcattttta gtgagtaatg ttggctggcg ccatagcatg ctgattatcg
      541 ccttcttatt tattgcctta tcaggattat tgtatcaatt tgtgcaagat aaacccggtg
      601 agcatcgcaa tgaaattcgt tctgttaatc gaatcagtat tttggatagc ttaaagatca
      661 ttttatcaaa taaacaaact tggcttaatg cgatgtatgc tggattttta tttggcccca
      721 ccgctgtaat tggagaggca ataggtccgg catatttaca gttcggccgt ggtttagggg
      781 cgcacgcagc tgcatttgca acaggattga tctttatagg ctggggaatt agtggcccat
      841 tatcaggttg gatttctgat aaaatgggac gtcgtaagcc attgatgatt atttccgcag
      901 tatgtggtgt gatcttgagc agtttatttg tttttattcc tgaaatgagt caaacaacgg
      961 cttacatact attttttgtt tttggtctta caaacactgg ggttgcgatt tcatacgctg
     1021 tttctacaga aatacatgac aggagtgttg ttggtacatc tatcgcattt accaatatga
     1081 cttcaatttt tgtcggggcc ttattccagc ctttagtagg acgtatcata gacatggtgt
     1141 ctgggcccag agcctataat gttgaaacgt tattattatc ggatttccaa gccggtctaa
     1201 aattactccc cttatgttcc ttggtggcct taatccttgc gtttactgtt aaagagactt
     1261 attgcaaacc cataaggcat taagagatag caaaaatcaa acattgagca ttgcatgata
     1321 aatatgccac cattgtttga tttttgctgt aaatacatca aatttatgaa atttaatgaa
     1381 aatatttttc attaagtttt aatcttcttg cattagactg tcgcactttt aggttatttg
     1441 ctgttatact tttcgcagca ctttgctgcg ctcgagtaat gctatttgca aattaataac
     1501 atggagataa taatgaaaaa acttacagga ttagtaatta tcctggctgt tttaatccta
     1561 ggtggatatt atggcatggg aattctaacc gaaagaacgg ttaaaaaaac tgtcgaggta
     1621 attaatcaat ccaatgggct tttcgctgac attcagcaat ataagagagg ttggttttgc
     1681 tctgatgcat tagttaaatg gcgtttacac gtacctgagc gagtagtaaa agacgatgac
     1741 ggaaaattgc aaactactcc tgctcaagat tatcaaatgg aaatgccgat taagatttat
     1801 catggcccca ttatatacgc gaataagcag ttacgttttg gaatgggctt tgcacaaacc
     1861 gtatttccat ttccagaaaa atataatcag caattcaatg agcttttttc tcaagattca
     1921 gaaaagcctc agctggattt aagcattttt gtaaattatt tgaataaaag tacagttgag
     1981 ttgtccattc cttctttcaa attaactgct aaagatggaa atggtaaatt taattggatg
     2041 ggtatggaca gtactacatc aatgtcttcc aatttggata aagtggaagg ggaaattgaa
     2101 tttgatggcg cagagttttc taaagatgat accaagctct ctttgggtaa ggtaaacagc
     2161 gagtatgact tacataaaac gactacaggg ttatacctgg gtcaagccag tttttcattg
     2221 ccatcacttg ctgtagttgt taaagatcaa aagatgtttg aaattagtga attgacatta
     2281 agctcaagca gcgatataga cgataatctc ttcagcacgc attttagtgc tgctttaaaa
     2341 tcactattcg ccaatgggca aaattacggt ccaggtgaag tggagatgtc cttaagaaac
     2401 cttgatgctg aagtattggc taaaatcaat gagcaagcta atgcgatgca aaggggcaca
     2461 gatcaagagc gtcaacaagc attgatggca atgctgccag aattgccaaa gttatttagt
     2521 aaaggtgcag agtttgaaat ttccaaatta agtctcaaac ttccacaagg catgattgat
     2581 ggtaatttgt taattacttt acccaaagga gatagttcta atccctttga attgattcaa
     2641 aaaactcaag gtaatgccaa gttaaaaatg ccaatcgagg tagtgaagca actgatgcag
     2701 caatcgatta tgcaacaaat ggcaaaacag ccagaaatgc aacaagcgtt aattcaacag
     2761 ctgcaaaaca gtcaaggtca atcaaaccaa gcagaaccta ctcctgagca aatcgctgca
     2821 atgcaagtag ataagcaaat taatgagttg gagcaatctg gtttaatcac tgtcgatggc
     2881 aaagactatg tcattgagat gagtttgagt gaaggaaaat tcacagttaa tgggaaacca
     2941 tttgatccat ctatgatgaa atttcagtag ttcgttagat ataggataag ctgttgagcg
     3001 cggcttatcc tcttttcaat ccactatggt gagaaacgtg gcaatgattc aagagcaaac
     3061 agtatatatt gaattttaat gcagtttttt ctgttctgcc cttgtctcgt attgctcaat
     3121 tcggtaatat accgctttta aaaggaggag ctaataataa tgacaacaat cagtaatgat
     3181 gcaggagata ccactgcttc actagccgaa agcgtgactc aatccgtaca aaaatatttt
     3241 tcagagctta agggaactga tcctgttgac ttataccagt ttgtacttga agaaattgag
     3301 acccctctat ttcgtgcagt aatggaacat tgcaagtata atcagtcccg cgctgcaatc
     3361 atgctgggaa ttagtcgtgg aactttaaga actaaattaa gacgttattt tgatgataaa
     3421 tacgttggta caagggatta agaaagattg ataataaaaa gggagcctta gctccctttt
     3481 tattatcaaa gaatatttca ttttattcag caaacatgga gcttactgac tcctcaacat
     3541 ttactcgttt gattgcttga gctaacatat cagcaagact gaccactctg attttttcac
     3601 agttttgtgc ttcggctgat aaaggaatgg tatcagttac taccacctca tcaagacctg
     3661 aatgtttaat attgttaact gcggggcctg acaacacagg atgtgtaatg taggctctga
     3721 cgctttttgc tccgtttttc tttaattcat gagcagccgt acaaagtgtt ccagcagtat
     3781 ctactatgtc gtctacaata atacagtttt tattggcggg ctcaccaata atgtgcatta
     3841 cttctgattt atttggccca ctacggcgtt tatcaataat cgacagctca gcgtcattca
     3901 gtcttttagc aactgctctg gcacgtacaa ccccaccgac atctggcgat acgatcatga
     3961 tattgtttaa tttttgtttg gttatatctt ccagcaatac cggagttgag tatacgttat
     4021 caaccggcat ataaaagaaa ccctgtattt gatccgcgtg caaatcaaca gtaaggaccc
     4081 gacaaattcc aactgaagcc atcatgtctg ctaccacttt agctgtgata ggaacacggg
     4141 cggagcgcac tcgcctatct tggcgtgcgt agccaaaata aggaactaca gctgtaatac
     4201 gaccagcaga tgatcttctc agggcatccg ccataatcag caattccatt aaattattat
     4261 ttgcaggcgc acaagttgat tggaggacaa aaacatcttt acctcggaca ttttctaaaa
     4321 tttcaaccat agtctctcca tcgctgaagg taccaacggt ggctttgcca ataggtattt
     4381 gcaaatgtga ggaaattttt agtgctaatt cagggttagc attccctgta aaaatcatca
     4441 tcgtggacat gtgtcaaaag ccttagattt aggttaatca cataacttaa acacatagca
     4501 gaagacagcc aaaaagatat tattattatt tgagcgatac gaagtacgca tccttccagg
     4561 ttatcttctg ccgtctgttt acttgtacct agattcaact tcatattcta ggttgaatca
     4621 acaagaaaat ggcaggggtg ctaggattcg aacctaggta tgcagggatc aaaaccctgt
     4681 gccttaccgc ttggcgacac ccctataatt gtagattctt caatcatcca tgagtttata
     4741 aatagtattc atccctgcaa tgtgctgcgt attttgcagt gcttttttat cgatgtcaac
     4801 ttcagaaagc aaataaatta caagaattaa attatacaac ccaattcttt atcactacct
     4861 ttaccatgac gccatttcct tcaaggcgaa tcatacttgg taaatctatt cccttgaccg
     4921 aagtatactt agtaaaatca atggtatagc cattttgctt taattgtatc aggtgattga
     4981 attggtcatg tttttctgat tggattccac ctggtgcggg taaacctctt acccagtaat
     5041 acaaattatt tactggaagt cgaatgccag tttgttttag taataactca tcggcattgg
     5101 tcgaagtagt tattttggcg ccatcttgaa atgtaatagt attccccttt ttgctgatta
     5161 gaacggctcc accacctaat ggccccatta aacgaatttg gtatgagttg gccccttgtt
     5221 ggacccaatt catagtagca gaccatcctt tcgctttggt ttttgcagcc agagcccctt
     5281 taatttccca tgaggaaaca gtggctgttt cggctttacg ttcttcaaga ggaatttcac
     5341 ggtttgcagg cggctcttca gagggtcttg ggggagcgca tgctgttaaa aagcaaatta
     5401 aaatgattgt caaacgtttt atcgcattca ttgcttacct caattccatt tttttatagt
     5461 ttttcaattt acaatacgct agactgcttc tatatgcaaa tggtaattaa cgagatgaaa
     5521 caaaaagcaa tctaccctgg aacatttgac cctgtaacca atggccatat tgatattatt
     5581 actcgggcca gcactatttt tcctgaattg atagttgctg tagcgagtaa taaaaacaag
     5641 cggccttatt tatcttggga aagcagaatt agtttactgg aagagtccgt tggacattta
     5701 actggcgtga gagttgtggg ttttgataat ttgctaattg attttgtatt agaacaaaac
     5761 gcaggcatta ttttacgtgg attgagagct gtttctgatt ttgaatatga atttcaactg
     5821 gctggaatga acagaaaact atctaaaaag gtagagacat tatttttaac ccccgcagag
     5881 catttaatgt atatctcatc cactttagtt cgtgaaattg cagccttaaa tggtgatatt
     5941 tctcaatttg
//
LOCUS       DASLOE010000171         5912 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.172, whole genome shotgun sequence.
ACCESSION   DASLOE010000171 DASLOE010000000
VERSION     DASLOE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.172"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(280..1128)
                     /locus_tag="U6309_002515"
     CDS             complement(280..1128)
                     /locus_tag="U6309_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0518 family Dot/Icm T4SS effector"
                     /protein_id="HEN5505943.1"
                     /translation="MTDLRTEIESLIHNGLLPDREYVSAKRFINHYANTNEFWAGLAL
                     VGITPIINGLYACYYASRAVWATLRTVGNLLILKPGLALDAIKDVGCHLTLSTCLLLM
                     APIHALTHAVEILTRVISSWFVGQEPRHDLLETGLMAKWANRVSGYQMVNELPSSNYF
                     NKARFFSSYNNAWDCLEQMASPMTSLAKGGFHSLVEAFYGVESAIECIVNLAICKPLH
                     AKENFHNLQVHFGLAVSLAVMTPINALVEGIAFMTRLGTTWVSACLKPEVTETEHENT
                     TAYQFN"
     gene            complement(1466..1933)
                     /locus_tag="U6309_002516"
     CDS             complement(1466..1933)
                     /locus_tag="U6309_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505944.1"
                     /translation="MNAENNVLNTLNSLSKEINKLYGYVTIAGDNFGEPAINSGPCGP
                     FANAFYKIWNQKFTEKVNIAFIMMKNSDECWHILIRLPNGLLFDGGFGVHNEDKWNKD
                     TFDIEDMLEYDLQLLEKHSGGLNRTYPRYCPDFSIDVITNLIHKHLELVEKNK"
     gene            complement(1917..2675)
                     /locus_tag="U6309_002517"
     CDS             complement(1917..2675)
                     /locus_tag="U6309_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5505945.1"
                     /translation="MTKLKNLDTYLSLCTEVYDLSKPNPPEEAYAFYRSYVMNVKDPI
                     LEPMCGTGRFLLPLLEEGFEIHGFDASEYMLEALNIKAKAKNLKPTVWRGFVENLTRP
                     EKYNLIFIPSGSFCLLIDPAKVRLALQTFYNHLSDEGILLFEGETLKAVPQLDVWRGS
                     VWHKPNGQMIMLSQLATLKDQVCHSIGKYELVHDNSIIHTEVEELRVRIYDPQELTAI
                     LKSCGFKNVRLIKAFDSAATPDAQDETIVYECRK"
     gene            complement(2641..3249)
                     /locus_tag="U6309_002518"
     CDS             complement(2641..3249)
                     /locus_tag="U6309_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN5505946.1"
                     /translation="MTPHIIGISGITGAGKSTLAKALSLDLKATLISWDDFDDISLEP
                     EDYIEWYHKGCNYSEFQRENLAKVLAELKAKREIMHPVLSTLLNPAEYIIFDAPLGKL
                     HIQTGEYIDTCVHIEVPLDISLCRRILRDFKDKLQTKENLLEEISFYLNHSRPLFFDD
                     ELKQNATIVIDGLLSTEVQIQIIKNFLIGKQDDKIKKPGYLS"
     gene            complement(3246..3713)
                     /locus_tag="U6309_002519"
     CDS             complement(3246..3713)
                     /locus_tag="U6309_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505947.1"
                     /translation="MITIDLLKNHPQSIARCATIWHEVLGKIWAPEIPVEQVIQRFSE
                     HLNADKLPITFIALDTGKPVGMCSLRENDGIRPEISPWLGSLVVDPAYQRQGIGNRLI
                     EATKNKARDLGFNKLHLFAFDPTIPNYYSRLGWTIIGMDKFKGHPVTVMEIVL"
     gene            complement(3710..4708)
                     /locus_tag="U6309_002520"
     CDS             complement(3710..4708)
                     /locus_tag="U6309_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEN5505948.1"
                     /translation="MNSNHIKRLCQHFNLGDPTQEPQRVSGGLLHIMWQLNTDKGSFA
                     IKQLSKDINLTDYHVIRNYELSENIASGFIALGIPAVTAIEKSGKHLFIINETGFLVY
                     PWVNAKALDKDAVSVSQALEIARILSKMHQLDLQCKEIEEAEFDIHERGKIIALIDLS
                     QKKKLPIASSLMENLVTILEINTNYHDAILMLKAHGVISHGDLDQKNVLWDSSKKPIL
                     IDWESARKLNPTYEIVNAALDWSGITTQLDKNLFGKMLRAYKEAGGVIDKDLVEAAFF
                     GVLGNWINWMVYNIQRAANHCVPEQQKIGMEQVMQVLPTVLRLKSLIPELVNAGKA"
     gene            complement(4705..5115)
                     /locus_tag="U6309_002521"
     CDS             complement(4705..5115)
                     /locus_tag="U6309_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosfomycin resistance glutathione transferase"
                     /protein_id="HEN5505949.1"
                     /translation="MITGINHITLAIKDLNKSFAFYANVLGFKPLVRWDKGAYFLAGD
                     FWFCLNVDANQRPTPCYTHYAFTVTQEQFDEMSKAIIQSGIKIFKENTSPDDSLYFLD
                     PDGHKLEIHVGNWQSRLIAKKINPGTWQNVEWFV"
     gene            complement(5112..5675)
                     /locus_tag="U6309_002522"
     CDS             complement(5112..5675)
                     /locus_tag="U6309_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5505950.1"
                     /translation="MDINFEKANSSHIDVIFSWLKEPFVQEFWDNTQTHKDDILNFIN
                     GRTEPSSYADGKYVYWIASSNGHPFAMLMTIQETSENEIDEIKLINLSKTGHSYGIDY
                     MIGDKNYFGIGYGARTLIEFIDFFIKEVDPKADTFLIDPASDNPRAKHVYIKAGFEYV
                     ADFLMSGNVSGAGKLHHLLIKRIGLIK"
     gene            complement(5704..5780)
                     /locus_tag="U6309_002523"
     tRNA            complement(5704..5780)
                     /locus_tag="U6309_002523"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5744..5746),aa:Pro,seq:cgg)
BASE COUNT         1812 a         1120 c         1084 g         1896 t
ORIGIN      
        1 aaaatctttt taatgaggga gtttagataa actaaatgac cgaattaaaa acatttttta
       61 acatagagat tggggttttt cgtaagtcct gttgttttca ttttcaatag ttacaaatcc
      121 ttacctaaaa gacaacaggc gggattaatt cgaaaggtta ttcgaagaac gtactactag
      181 ttttatggaa ttatacaagg aaatcagtcc ttaatcacaa agagcttggt attttttaat
      241 gagctacagt cgcaagcgac tgtagcaagc aggtaagaat tagttgaatt gataagcggt
      301 tgtattctca tgctcagtct cagtcacttc tggtttgagg caagcagaaa cccaggtagt
      361 accaagacgt gtcataaagg caattccttc gaccagagcg ttaattggtg tcatcacagc
      421 caaagagaca gccaaaccga aatgtacttg taagttatgg aaattctctt ttgcatgtaa
      481 aggcttacag atagccaaat tgacaataca ttctatcgca gactcaactc cataaaatgc
      541 ttcgactaat gaatgaaatc cgcctttagc caaggaggtc attggggatg ccatttgttc
      601 taaacaatcc caggcattgt tataagaaga aaagaacctg gctttattga agtaattgga
      661 tgaaggcaac tcattaacca tttgatatcc acttactctg tttgcccact ttgccataag
      721 cccggtttct aataaatcat ggcgaggttc ttgaccaaca aaccatgatg atataactct
      781 ggtaagaatt tcaacggcat gagtcagagc gtgaattgga gccataagca gtaaacaggt
      841 agagagagtt aaatggcaac ccacatcttt gatcgcgtcc aatgccaagc ctggttttaa
      901 aattaataaa ttacctacag ttcgcaaggt ggcccatact gcccttgagg catagtagca
      961 agcatataac ccattgataa taggggtaat tcctactaat gccaaaccag cccaaaattc
     1021 atttgtattg gcataatgat taataaatcg cttggctgaa acatattctc tatctggcaa
     1081 taatccattg tgaataagcg attcaatttc tgttctaagg tctgtcatta tggtctctct
     1141 ctatgttaat taaacaagcg gggagagtat aaccaaaaaa aacataaatg accaatattg
     1201 gcccatgttg tatttgatgt ttgcatctgt tttccaaggt cttttgaaca tagaccaaag
     1261 gccatagaat caataatact cttattgggt ataactcatt gaataaattt tctttattat
     1321 ggcgtacgct ccaatcggcc ataatgcagc acatattgtg atggaaaaat gcggtatgaa
     1381 atactacaaa gatggagtgg ggcatgaaat acaaggtaag ttttatcgaa ttaaaaacaa
     1441 taaatagact ttgggtagca aattgtcact tatttttttc aaccagttcc agatgtttgt
     1501 gtattaaatt ggtaatgaca tcaatcgaaa aatcagggca ataccggggg tatgtcctgt
     1561 ttaaaccacc agaatgcttc tctaaaagct gaaggtcata ttccaacata tcttctatat
     1621 cgaaagtgtc tttattccat ttgtcttcat tatgaacacc gaaacctcca tcaaaaagca
     1681 acccattagg taatcggatt aagatatgcc agcattcatc agaatttttc atcataataa
     1741 atgcaatatt tactttttca gtaaattttt gattccaaat cttgtaaaaa gcattggcaa
     1801 atgggccgca agggcctgag ttaattgcag gttcgccaaa attgtcccca gcaatggtga
     1861 cgtacccata gagtttattt atttctttag ataagctatt tagggtatta agaacattat
     1921 tttcggcatt cataaacaat tgtctcatct tgggcatctg gagttgcagc actatcaaat
     1981 gctttaatca aacggacatt tttaaaccca caacttttaa gaattgctgt taactcctgc
     2041 gggtcgtaaa ttcttaccct caattcttca acctccgtat gaattatact attgtcgtga
     2101 acgagttcat atttaccgat agaatgacaa acctgatcct tcagggtcgc taattggctg
     2161 agcataatca tctggccatt gggcttatgc caaactgagc ctctccagac atcgagttgt
     2221 ggcactgctt tgagtgtttc gccctcaaac aataaaatac cctcatcgct taggtgatta
     2281 taaaatgttt gaagtgctag tcttactttt gcaggatcga taagcaagca aaaggaaccg
     2341 cttggtataa aaatcaaatt gtatttttct ggcctcgtta aattttcaac aaaacctctc
     2401 catactgttg gtttgagatt tttagctttg gctttaatat tgagggcttc tagcatatat
     2461 tcgcttgcat caaagccatg tatttcgaat ccttcttcaa gtaatggaag taaaaaacga
     2521 ccggtaccac acattggctc taaaataggg tctttcacat tcattacata actgcgataa
     2581 aatgcatatg cctcttcagg aggattgggt ttgcttaagt catagacctc agtgcacaag
     2641 ctaagataag tatccaggtt ttttaatttt gtcatcttgt ttaccaatta aaaaattttt
     2701 gattatttga atttgcactt cggtacttag gagaccgtca atgactatag tggcattttg
     2761 tttcagttca tcatcaaaaa acaaaggtct tgaatggttt aaataaaaac tgatttcttc
     2821 aagaagattt tctttagttt gtaatttatc tttaaaatcc ctcaaaatgc gacggcacaa
     2881 tgaaatatca agtggcacct caatatgaac acaggtatca atatattcac ctgtttgtat
     2941 gtgaagtttc cctaagggag catcaaaaat tatgtattcc gcagggttga gcaaagtgct
     3001 taagactgga tgcataattt ctctttttgc tttaagctct gccaaaacct ttgcaaggtt
     3061 ttccctttga aattcactgt aattacaacc cttatgatac cactcgatat aatcttcggg
     3121 ttctaaagaa atgtcatcaa agtcatccca tgaaataaga gttgctttta aatctaggga
     3181 caatgctttt gccaaagtgg attttccagc gcctgttatg ccgctaatgc caataatatg
     3241 aggagtcata acactatctc cattacagtg actggatgtc ctttaaattt atccatgccg
     3301 attatggtcc aaccaaggcg ggagtaataa ttaggaatgg ttgggtcaaa ggcaaaaaga
     3361 tgcagtttat taaaacctaa atctctggct ttattttttg tggcttcaat tagcctgttt
     3421 ccaatacctt gtctctgata ggcagggtca accacaagcg agccaagcca tgggcttatt
     3481 tctggcctga taccatcatt ttcacgcaaa gagcacatcc caacgggttt ccctgtatca
     3541 agtgctataa acgtaatggg tagtttgtca gcatttaggt gctcactgaa ccgttgaata
     3601 acttgttcaa ccgggatctc gggagcccag attttcccaa gtacttcatg ccaaattgtc
     3661 gcacaccttg ctatgctttg tggatgattt ttgagaaggt cgatggtgat catgctttac
     3721 ctgcattgac gagttcaggt attaagcttt tgaggcgtag aacagtaggt aacacttgca
     3781 ttacttgctc cattccaatt ttttgttgtt ctggaacgca atggtttgca gcccgctgga
     3841 tgttatagac catccaatta atccaattac ctaaaacacc aaaaaaagca gcttcaacta
     3901 agtctttatc aattacccca cctgcttctt tgtatgctct tagcattttg ccaaatagat
     3961 ttttatcaag ttgtgtagta atcccagacc aatctaacgc ggcattcaca atttcatagg
     4021 tagggttaag cttgcgtgcc gattcccagt ctatgaggat gggttttttt gaggaatccc
     4081 ataagacatt tttttgatcc aagtcgccgt gactaattac gccatgtgct ttaagcatta
     4141 aaattgcgtc atgataattt gtattaattt ctaatattgt tactaaattt tccatcaagg
     4201 atgaagcgat agggagcttc tttttttggg ataaatcgat tagagctata attttgcccc
     4261 tttcatgaat atcaaattct gcttcttcaa tttccttgca ctgcaaatcg agctgatgca
     4321 tcttagataa aatccgagca atttccaaag cttgagatac acttacagca tccttatcaa
     4381 gggctttggc attgacccat gggtaaacca gaaaaccagt ctcattaatt ataaaaagat
     4441 gtttacctga tttttcaata gcagtaacag caggaatacc aagcgctata aagcctgagg
     4501 caatgttttc acttaattca taatttctaa taacatgata atctgtaaga ttaatgtctt
     4561 ttgataattg tttaatcgca aaagaacctt tatcggtatt tagctgccac atgatgtgaa
     4621 gtaagccgcc agatactctt tggggttctt gtgttggatc acctaaatta aagtgttggc
     4681 atagacgctt gatatggttt gagttcatac aaaccactcc acattttgcc atgtacctgg
     4741 gttgattttt tttgcaataa ggcgtgattg ccagtttccc acatggatct ctaatttatg
     4801 gccatcagga tcaagaaaat aaagtgaatc atctggggag gtattttctt taaagatttt
     4861 aatgcctgat tgaattatcg ccttagacat ttcatcaaat tgctcttgcg taactgtaaa
     4921 ggcataatga gtatagcaag gtgtaggacg ttggtttgca tcgacattta aacaaaacca
     4981 aaaatcgcct gctaaaaaat aagcgccttt atcccatcta accaatggtt tgaacccaag
     5041 gacgttagca taaaaagcaa aagacttatt gaggtcttta attgctaaag taatgtgatt
     5101 tattccggta atcatttgat taaaccaatg cgtttaatca ataaatgatg aagttttccc
     5161 gcgccagaga cgttacctga catcaaaaaa tcagcaacat actcaaaacc tgcttttata
     5221 taaacatgct tggctctggg attgtcactt gcgggatcaa ttaaaaacgt atcggctttc
     5281 ggatcgactt cttttataaa aaaatcaata aattctatca aggttctggc tccataacct
     5341 atgccaaaat agtttttatc accaatcatg taatcgatac cataagaatg tccagtcttg
     5401 gaaaggttaa tcaatttaat ttcgtcgatc tcattttcgc ttgtttcctg gattgtcatc
     5461 agcattgcga aaggatgccc attactgctt gctatccaat aaacatattt accgtctgca
     5521 taggatgagg gttctgtgcg gccattgata aagtttaaga tgtcatcttt atgagtttgt
     5581 gtgttgtccc agaactcttg aacaaagggt tctttaagcc agctaaagat aacatcgata
     5641 tgacttgagt tggctttttc aaaatttatg tccattaggc aaattctaat tctttgattt
     5701 tattggtcgg agtgacagga attgaacctg cgacccctgc ctcccgaagg cagtgctcta
     5761 ccagtctgag ctacactccg cgtttatttg aagtttatta attaatttaa tggtttaaga
     5821 aagtatacga attattttac ttgaagtgaa tttatccgtc gatttattgt tgatttgagt
     5881 acttaaattt acctcaaaat aacccttaat tt
//
LOCUS       DASLOE010000172         5883 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.173, whole genome shotgun sequence.
ACCESSION   DASLOE010000172 DASLOE010000000
VERSION     DASLOE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.173"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..719
                     /gene="yaaA"
                     /locus_tag="U6309_002524"
     CDS             <1..719
                     /gene="yaaA"
                     /locus_tag="U6309_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEN5505951.1"
                     /translation="KETSNPLLLDKALQLVKIMKLKSVEQIADLMDLSRQLAELNYER
                     YQNFDLKNNPMNHSYPALFLFQGDVYQGLNANSWKDEEIEYAQSHLGILSGLYGFLRP
                     LDRIQPYRLEMGVNLENPAGKNLYTFWSKIVTNILNQILVEQSNPVLINLASTEYFKV
                     VDEKKLSYPIVTINFYEQKNSELKMIGILAKKARGMMAKYIMQNRIDSIEQIKEFSES
                     GYLFNKEISSPNSLNFIRIH"
     gene            740..1639
                     /locus_tag="U6309_002525"
     CDS             740..1639
                     /locus_tag="U6309_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEN5505952.1"
                     /translation="MWNLSSRVHALNHFPKQGIECYVKRDDELGCGISGTKIRKYSSL
                     IPFLKIKGIRHLIIIAGAQSNNLLAALQVARECQLKVTAFLVKPKHLKIQGNFKLSLL
                     FLHENEIIWINREEWYRVNEFAEQYLEGLRENGYILSEGASVKESMKGAMSLANDIKE
                     NEKILGFAFDHIFVDAGTGFSAIALIKGFHELQHKGFIHVLLLADSEEVFKKKLMHWA
                     GVNPDNYTCFYPSTAKSFGSVNQTIKQEIKRLAYEEGILADPIYSAKLFYEARRYIEK
                     YELKGKVLIVHSGGILTMPGFAF"
     gene            1852..3429
                     /locus_tag="U6309_002526"
     CDS             1852..3429
                     /locus_tag="U6309_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase Lgt1"
                     /protein_id="HEN5505953.1"
                     /translation="MKARRDQQLSKLRMRFFSALNHTSEIDLHMLFNDLKSILTLDSI
                     EHLKEGSVAYAIIQELLKQDDAQNKIQSFLHGAIKNVIHPGVIKGLTPDEINWNVAKA
                     YPKYYEHEEFPDVTFGGFKVRDSNEFKFKTNIQTSIWFSIKPDLFMPSKQQEALKRRR
                     EQYPGCEIRLIYSSSLLNAEANRQMKAFARKQNISLIDIDSVKTDSPLYPLLKSELAH
                     LGKGGNPAAASDLCRWIPELFNEGFYVDIDLPVDSSKIVEGHQITGGVPIMLNMGSII
                     SEPIAPHHRRQEAVCMNTDIIAYSNDKRTQKMMDTVARHLKNIYDDPYTALKDTPLAQ
                     TAFFNKCKEEKKSIFDLRKGLQDAFRSDSLLQLYDFLGADKFKEVFKLKEAQSKYINE
                     HISEFSEKDLLLNLISDKPSEINQHTLDFVKAKAMYIDIAKEHYSAFYKPLVEEISGP
                     GAIYNALGGAGSFTTTHRRSTGPMLPTTPPRVLQVFCDAHDKGPFVSDNIARWQTNVR
                     DLGVLNREGLSWLPSVG"
     gene            3632..5503
                     /gene="htpG"
                     /locus_tag="U6309_002527"
     CDS             3632..5503
                     /gene="htpG"
                     /locus_tag="U6309_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEN5505954.1"
                     /translation="MVSKQQTMGFQTEVKQMLHLVVHSLYSNKEIFLRELISNASDAL
                     DKLRFLALSNGSLFENDSDLKISIQINEKLQTITISDNGIGLSWEEAVENLGTIAKSG
                     TKEFISQLTGEQAKDSQLIGQFGVGFYSAFIVADKVTVKSRRAGLQPEDGIVWESKGD
                     GEFTIGYEKKLTRGTEITLHLKPENDEFLSDWRIRGIISKYSDHICWPIVMKKLSEEG
                     KESKEFETVNKATALWTLQKSEISEEEYKQLYKHISHDYMDPLTWSHNHVEGKHEYIT
                     LLYIPAHAPFDLWQHEAKHGLKLYVKRVFIMDEATQFLPRYLRFVKGIVDASDLPLNI
                     SREILQDNKQVESIRAACTKRVLSMLEKMATNDKETYQKFWNEFGLVLKEGPIEDFAN
                     KEAIAKLLRFSTTASGSEKQEVSLEEYVSRMKEGQDKIYYITASSYNAAKNSPHLEIF
                     RKKGIEVLLLSDKVDEWLVGYMNEFAGKKLQSISKGKIELGDDETSEQIKEQEKTLEP
                     LIKHIKSVLNDRVKDVLLTNRLTDSPACVVADEQDMGLEMQRILQAAGQQVPVSKPIF
                     EINPDHALIKRLHDIQDDNQFELWVTMLFEQAVLAEGGQLDNPADFVNRVNRLLVSS"
     gene            complement(5615..>5883)
                     /locus_tag="U6309_002528"
     CDS             complement(5615..>5883)
                     /locus_tag="U6309_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEN5505955.1"
                     /translation="QSTKQEQGLQDLVYSLVTRFLAENKSKSINDLYDMILSEVEPPL
                     LQAVMEKRRGNQLQAAKMLGISRGTIRKKLQRYFGTKYFRLTEE"
BASE COUNT         1953 a          934 c         1192 g         1804 t
ORIGIN      
        1 ctaaagaaac cagtaatcct ttattgctcg ataaagcgct acaattggtt aaaataatga
       61 aattaaaatc tgtggaacaa attgcggatt taatggattt atcaagacaa ctcgctgaat
      121 taaactatga gagatatcaa aattttgatc ttaaaaacaa cccaatgaat cactcttatc
      181 ccgctttgtt tttatttcag ggggatgtgt accaaggatt gaatgctaat tcatggaaag
      241 atgaagagat tgaatacgcc caatcccatt taggaatttt gtcaggacta tatgggtttt
      301 tacggccttt agataggatt caaccttaca gattggagat gggagttaat ttggaaaatc
      361 cggcaggtaa gaatctctat acattttgga gcaaaattgt tactaatatt ttaaaccaaa
      421 tactggtaga gcagagtaat cctgtactta ttaacctggc ttcaactgag tattttaaag
      481 tcgtggatga gaaaaaattg agttatccta ttgtgactat caatttttat gaacagaaaa
      541 atagcgaatt aaaaatgata ggtatccttg ctaaaaaagc aagaggaatg atggcaaagt
      601 atataatgca gaatagaata gactccatag aacaaattaa agagttttct gagtcgggtt
      661 atttatttaa taaagaaatt tccagtccaa atagtttgaa ttttattcga atccattaaa
      721 ttatttataa aggtatttga tgtggaatct gtccagtcgg gtgcatgccc ttaatcattt
      781 tcccaagcaa ggtatagagt gttatgttaa gagagatgat gaattaggtt gcggcataag
      841 tggtacaaag ataaggaaat acagcagttt aatcccgttt ctaaaaatca agggaattcg
      901 ccatctcatt attattgcgg gtgctcaatc taataattta ttggctgctc tgcaggtggc
      961 cagagaatgt caattgaaag tgacagcctt tctagtaaag cccaaacatt taaaaatcca
     1021 gggtaatttt aaattaagcc ttctttttct tcatgagaat gaaattatct ggataaatcg
     1081 agaagaatgg tatcgagtca atgaattcgc tgaacagtat cttgaggggc tcagagaaaa
     1141 tggttatatt ttatctgaag gtgccagtgt taaggagtcc atgaaggggg caatgtctct
     1201 agccaatgat atcaaggaaa atgaaaagat tttgggattt gcttttgatc atatttttgt
     1261 tgatgcaggt acaggttttt cagcaatcgc gctaattaaa ggttttcatg aattacagca
     1321 taaaggattt attcatgtgt tattgttagc agatagtgag gaggttttta agaaaaaact
     1381 tatgcattgg gcaggagtaa accctgataa ttacacttgt ttttacccaa gcactgcgaa
     1441 atcgtttgga tctgtaaatc aaacaattaa acaagaaatt aaacgattgg cttatgaaga
     1501 aggcattttg gctgatccga tttactctgc aaaattattt tatgaggcca gaaggtacat
     1561 agaaaaatac gaattaaaag ggaaggtatt gattgtacat tctggtggta tattaaccat
     1621 gcctggcttt gctttttgaa taggcttgct tcttggcaaa gacgtaattt ctgaattatc
     1681 acgttttcct gactgcaaaa ttaagttcat caaggatatt cagtatattt cagtacataa
     1741 tgcgattttt ttcctgaata tctctgtctc gattatctac tatacttatc aattataaaa
     1801 ataaaaaccg ttctttttaa gaaaagttat cttatatggg agacgagtat catgaaagca
     1861 agaagggatc aacaactctc taaattgcga atgagatttt tttcagcgct caatcacaca
     1921 tctgaaattg acttacacat gctttttaat gatttgaaat caattctcac actggatagt
     1981 attgagcatc tcaaagaagg ctctgtagcg tatgcaatca ttcaagaact cttgaaacag
     2041 gatgatgctc aaaataagat tcaatctttt ttacatggag ctatcaagaa tgttattcac
     2101 ccgggtgtca tcaaagggct tacccctgat gaaattaatt ggaatgttgc caaagcatac
     2161 ccgaaatatt atgagcatga ggaatttcct gatgtgactt ttggcggctt taaagtacgt
     2221 gatagtaatg aatttaaatt taagactaat atacaaactt caatatggtt tagtatcaag
     2281 cccgatcttt tcatgccttc taaacaacag gaggctttaa aaagaagaag ggaacaatat
     2341 cctggttgcg aaattagatt aatttattcc agttcattgc taaatgcaga agcaaaccgc
     2401 cagatgaagg catttgccag gaaacaaaat atttcattaa ttgatattga tagtgttaaa
     2461 accgattcac cattatatcc attactaaaa tctgaacttg cacaccttgg aaaaggagga
     2521 aatcctgctg ctgcttccga tctgtgtcgc tggatccctg agttattcaa tgaagggttt
     2581 tatgtggata ttgatctccc tgttgatagt tcaaaaatag tagaaggaca tcaaattaca
     2641 ggaggagttc caatcatgct caatatgggc tccattatca gtgaacccat agctccacat
     2701 catagaaggc aagaagctgt ttgtatgaat acggacatta ttgcttattc caatgataaa
     2761 agaactcaaa aaatgatgga tacagtcgcc cgccatttga aaaatatata cgatgatcca
     2821 tatacagcat taaaagacac acctcttgcg caaacggctt tttttaataa gtgcaaagag
     2881 gaaaagaaaa gtatatttga tttaagaaaa ggattacaag atgcatttcg ctccgattca
     2941 ttattgcaat tatacgactt tttaggggca gacaaattta aggaagtatt taaattaaag
     3001 gaagctcaaa gcaaatacat taatgagcat ataagtgaat ttagcgagaa ggatctgttg
     3061 ttaaatttaa tttctgataa accttccgag attaatcagc atacacttga ttttgtcaag
     3121 gctaaagcaa tgtacataga tattgccaaa gagcactatt ctgcattcta caaaccatta
     3181 gttgaggaga tttcagggcc aggtgccatt tataatgctt taggaggagc gggcagcttc
     3241 actaccactc ataggcgttc aacaggacct atgttgccaa caaccccacc acgtgtttta
     3301 caggtatttt gtgatgcgca tgacaagggt ccttttgttt cagataatat tgcgcgttgg
     3361 caaaccaatg tccgagatct gggcgtgttg aatcgagagg gtttgagttg gttgccttca
     3421 gtagggtagc ttaatttcag cacacaaact ttgaatattg tctcaagcat tagcaatatg
     3481 aataatcacc aatagataaa agactttatt ggtgattagt gttttttgct ggtttcctcc
     3541 ttttttagcg ctatcccctt gaaatttaat agatcaaccc tatatgagaa gttaattatt
     3601 cgaacacaac tattaacggg gagattgttt aatggttagc aagcagcaaa ctatgggatt
     3661 tcaaacagag gtaaagcaaa tgcttcattt ggttgtgcat tcgctttata gcaacaagga
     3721 gatttttctt agagagttaa tttctaatgc ttctgatgcc ttggacaagt tacgattttt
     3781 agctttatca aacggttcat tgtttgaaaa tgattctgat ttgaaaatta gcatccagat
     3841 taatgaaaag cttcaaacca ttacaattag cgacaatggg attggcttaa gttgggaaga
     3901 ggccgtagaa aatttgggga caattgcaaa atctggaaca aaagaattta ttagccaatt
     3961 aactggtgaa caggcaaaag attctcaact cataggacaa tttggagtgg gcttttattc
     4021 cgcgtttatc gttgctgata aagtaacagt taaaagtcgt cgtgctggat tacaacctga
     4081 ggatggaatt gtatgggagt ctaagggtga tggtgaattt actataggat atgaaaaaaa
     4141 attgacccgt ggtacagaaa ttactttgca cttaaaacct gaaaacgatg agtttttaag
     4201 tgattggcgt attcgcggta ttattagcaa atactctgat catatttgtt ggcctattgt
     4261 gatgaaaaag ctttcagagg aaggcaagga atcaaaagaa tttgaaacag tgaacaaagc
     4321 gacggcactt tggacactac agaaatctga aattagtgaa gaagaatata agcaactgta
     4381 taaacatatt tctcatgatt acatggatcc attaacctgg tctcataacc atgttgaggg
     4441 aaaacacgag tatattactt tgctttacat accagcccat gccccatttg atttatggca
     4501 gcatgaagcc aaacatggac taaagcttta tgtaaagcgc gtatttatta tggatgaagc
     4561 gacacaattt ttaccccgat atttacgatt tgtaaaaggg attgtggatg ccagtgattt
     4621 accgcttaat atttctcgag aaatattaca ggataataag caagtagagt caattcgagc
     4681 tgcatgcacc aaacgtgttt tgtctatgct tgaaaaaatg gctaccaatg ataaagaaac
     4741 ctaccagaag ttttggaatg aatttggact tgtgctaaaa gaaggaccta ttgaggattt
     4801 cgctaataag gaagcgattg ccaagttgct tcgtttttct actacagcaa gcggtagtga
     4861 gaaacaagaa gtatcgctgg aggaatatgt tagtcgcatg aaagaagggc aggataaaat
     4921 ttattatatt accgcatcca gctacaatgc tgctaaaaat agccctcatt tggaaatatt
     4981 caggaaaaag ggaattgagg tattattgct cagtgataaa gtcgatgaat ggcttgttgg
     5041 ctatatgaat gagtttgcag gcaaaaaact gcaatcaatt tcaaaaggca agattgaatt
     5101 gggggatgat gaaacttccg aacaaattaa agaacaggaa aaaaccctgg agccattgat
     5161 taagcatatt aaatcagttc taaatgatcg agttaaagat gtattgctga ctaatcgtct
     5221 tactgattcc cctgcatgtg ttgttgctga tgagcaagac atgggattgg aaatgcaaag
     5281 aattttacaa gctgcgggtc aacaggttcc agtgagcaag cctatttttg aaattaatcc
     5341 ggatcatgcc ctgattaagc ggttgcatga tattcaggat gataaccaat tcgaattatg
     5401 ggttactatg ctgtttgaac aagctgtgtt ggcagaagga gggcaattgg ataatcccgc
     5461 tgattttgtg aatcgggtta acagactcct ggtttcctcc tagtattcca ttgggctggc
     5521 aatcaatccc ggatgagaac aagtcattat ccgggatgag ctgaacataa aataaaaagg
     5581 ctcaaaaatg agccttttta tttttaaatt tcccttactc ttcagttaag cgaaaatatt
     5641 tggtaccgaa atatcgttgt aatttctttc tgatcgtacc gcgactaata cccaacatct
     5701 tggctgcttg taattgattt ccacgacgtt tttccattac agcttgtaat agaggtggct
     5761 caacttctga caggatcata tcatagagat cattaattga tttggattta ttttcagcaa
     5821 gaaagcgggt aaccaaactg taaactaaat cctgtaaacc ttgttcttgt ttagtggatt
     5881 gag
//
LOCUS       DASLOE010000173         5859 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.174, whole genome shotgun sequence.
ACCESSION   DASLOE010000173 DASLOE010000000
VERSION     DASLOE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.174"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            119..634
                     /locus_tag="U6309_002529"
     CDS             119..634
                     /locus_tag="U6309_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5505956.1"
                     /translation="MDLIFSLGVMIMPLTPPASPKTERRSEEDNQQTNQQGNEQGNQQ
                     PRSSILTLFGFTPNALVMPCPHGHGHFHIMDIQADDTLVFATMNGSPEIMNLLALQFL
                     MQNINLNLDDILAAETDAKEAEEKEEPELSSSESVNEEKEESEFSSSESESESENEEK
                     EENEESTRFTM"
     gene            978..1862
                     /locus_tag="U6309_002530"
     CDS             978..1862
                     /locus_tag="U6309_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN5505957.1"
                     /translation="MTKTDKKIVVVTGCSKTSGVGYNLVCEMLKRGHQVIATVRNLET
                     SQINKANLPNSSNLDVKILDLCDENSIDRFIKEVLTQYQYIDALVNNAANVAIGPVES
                     ATTQDVLTTFQTKVFGPLSLIRGFIPVMRERKKGLLTTTSTIFTSVPVSIPGVAIYFS
                     ALAAFERLQESLAIELAPWNIKVINFQPGPIATELTRFEGSRSDIVEKYYKNFTEQAY
                     QWFHQNTIFQNAAEVAKVFAEVIDMENPDLYYQSNLFGKQFVAKNKKDTTGREGMNEL
                     LEHFKNLSHDNPDDWKIQ"
     gene            2086..3180
                     /gene="potA"
                     /locus_tag="U6309_002531"
     CDS             2086..3180
                     /gene="potA"
                     /locus_tag="U6309_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HEN5505958.1"
                     /translation="MTTPLIEIRQIYKSYGNTPILNNVSLNVNHGEFLTLLGPSGCGK
                     TTLLRLISGFEQPTQGEIFINGQCVNQLPPQKRDVHTVFQSYALFPHLSVFENVAFAL
                     RCKKTSNQEIKERVFDALKLVQLESLADRNVKQLSGGQQQRIAIARAIINRPQVLLLD
                     EPLSSLDYRLRKAMQSELKQLQKTLNMTFIFVTHDQEEALSMSDRIAVFNHGHIEQIG
                     TPKAVYETPANLHVAMFIGEANIFDIQVHTVKDQDIVTNIEGIQLSCKNTGNYQVNDR
                     LHLIVRPEDIRVWSLSEVEKREGMLPGRIVDIIYKGSTVDLKVELSSGKIINASEFFD
                     EDDDKLEYTLHETVWVQWLPGWEVLLPYEG"
     gene            3170..3994
                     /locus_tag="U6309_002532"
     CDS             3170..3994
                     /locus_tag="U6309_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN5505959.1"
                     /translation="MKAKSSALYLLYFWLIIFGFIPLLMVLVASFLSQDSIHLVTLPL
                     TLENYFDLFTPVFAKILFRSLLIACVTTLLCLLIAYPFSYLMIKSKHQSILLLLIIIP
                     FWTSSLIRTYALIAILKYKGVINALLLKLHLIDVPLSLLYTNFAVVIGLVYNLFPFMV
                     LPIFTNMERFDFRLIEAAKDLGANKWSIFTRVFLPNTAPGVLSGCLLVLLPAMTLFYI
                     PNVLGGARSILLGNLIQNQFLVLSDWPQGAATSVLLTSVLLILMFIFRRQNNEVIR"
     gene            3991..4758
                     /locus_tag="U6309_002533"
     CDS             3991..4758
                     /locus_tag="U6309_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN5505960.1"
                     /translation="MKTMTQRLFLSLVYAFLYIPIAILVLYSVNDAKFSLQWHGFSLR
                     WYKELFHDRGLWSAVFNSITLGLTSSLIATTMGLLASINLFLFRTRHRQILYTLLLLL
                     IIIPDIVLGVALLIFFNITQVPLGFLSLLVAHITFSIPFVILTINSRINTLNPNIYFG
                     ALDLGASRYFALTRVLLPLLWPAVLSAFLLSFTLSFDDVIISYFVAGPDFTILPLTIY
                     SLVRAGVTPELNALCSITFGLSMILVIISHRLSGKLI"
     gene            4755..5777
                     /locus_tag="U6309_002534"
     CDS             4755..5777
                     /locus_tag="U6309_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN5505961.1"
                     /translation="MKIFSLLLALLTFKAFSNPVVNVYAWGGEIPKKIIHQFEQESGI
                     KVNFSTYDSNETMYAKLRASQKSIYDVILPSAYFVERMQKQGMLQLLDHKQLSNLHNL
                     DEQFVKNDYDPGNRYSIPIIWGATGIFFNHNSVSNPPKSWQGLWDKKWRNQLMLLDDS
                     REIFAIALMSLGFDPNDTNPNHIKAAYEHLLQLVPNIKLFASDSIQAIMIDEDAFIGS
                     AWNGDAFKAHTENKAIEFIYPQDGFVIWVDCLAIPKNPPHLKEAYQFINFILKPETSA
                     QIALKEGHAITNAAGRALLPLPIKNNPVVYPPDKTLKKGYFQRDIGEETLALYNQYWE
                     QLKLTF"
BASE COUNT         1798 a         1137 c         1063 g         1861 t
ORIGIN      
        1 ttttttattc aataagaaaa atttgtttaa ttactctacc aagctctgta aattaattgt
       61 aaacttgacg ccgaaaagat agatttttaa ttagattatc ttttttgggc agtatttttt
      121 ggatttaatt tttagtttag gagtaatgat tatgccttta accccccccg cttcaccaaa
      181 aactgaaaga cgttctgaag aagataatca acaaactaat caacaaggaa acgagcaagg
      241 aaatcaacaa ccaagaagca gtatattgac tctttttggt tttactccta atgctctagt
      301 catgccatgc cctcatggcc acggtcactt ccatattatg gatattcagg cagatgatac
      361 attagtattt gctactatga atggcagccc ggaaataatg aatctattag cgcttcaatt
      421 cctgatgcaa aatatcaact taaatcttga tgatatactg gcggcagaaa cagatgcgaa
      481 agaagcggaa gaaaaagaag aaccagaact gtcatcttct gaatcagtga atgaagaaaa
      541 agaagaatca gaattttcat cttctgaatc agaatctgaa tcagaaaatg aagaaaaaga
      601 agaaaacgaa gagtcaaccc gttttacaat gtaatatgcc tgccctcttt tgaagggaca
      661 tcccgtattt acctgaggta aatacgggat ttttttaatt tctctgtctc caatattgtc
      721 aatctttatc agaatgtccc aataatcaaa cgagagcaat taatctcggt aataaactat
      781 atatcactga atatccttta ttttctcaac caatattttt ctgctttcaa aaaatcagtt
      841 aagagtttag aaaatcgata ataaatgacc gaattaaaaa ccttttttaa caaagagatt
      901 gggatttttc gtaagttctg aataaataaa agggctatac tcagtttatt actctaaaag
      961 atcattagga tagggacatg acgaagacgg ataaaaaaat agtggtggtc acaggttgtt
     1021 caaaaacatc cggcgtggga tataatcttg tatgtgaaat gctaaagcgc gggcatcaag
     1081 tcattgcaac ggtgagaaat cttgaaacaa gccaaattaa caaagcaaac ctcccaaact
     1141 ccagcaattt ggatgtaaaa atattggatc tttgcgatga aaattcaatt gaccgcttta
     1201 ttaaagaagt cttaactcaa tatcaataca ttgacgcatt ggttaacaat gctgctaatg
     1261 tggctattgg ccctgttgaa agcgcaacca ctcaagatgt attaacaact tttcaaacca
     1321 aagtatttgg cccattgtct ttaatcaggg gattcatccc tgtaatgaga gaaaggaaaa
     1381 aaggtttatt aacaacgaca tccacgattt ttacctctgt tcccgttagt attccaggtg
     1441 tcgcaatcta tttttcagca cttgcagcat ttgaacgatt acaagaatcc ttggcgattg
     1501 agctggcccc atggaatatc aaagtaatta atttccaacc tggaccaatt gcgacagaat
     1561 tgacccgatt tgaaggctct cgctctgata ttgttgagaa atactataaa aactttactg
     1621 aacaagctta tcaatggttc catcagaata ctatttttca aaatgcagca gaagtagcca
     1681 aggtgtttgc tgaagtcatt gatatggaga atcccgattt atattaccaa agcaatcttt
     1741 ttggaaagca gtttgttgca aaaaacaaaa aagatacaac agggcgtgag ggaatgaatg
     1801 aacttctgga acatttcaaa aacttatctc atgacaatcc tgatgattgg aaaattcaat
     1861 aaatgattta ctggataaag tcatactatc cgccgcgaaa tctctgtgca taagcaaaaa
     1921 atcggtcatg cccgcatagg cgctgagtga tttttttgct catgtacaaa acacaaaaat
     1981 agacaatagt tgactaattt tttaatattg ggcacaaagt ttgtgattga ctggacaaga
     2041 taagtcacag cacacataat tgcagttttt tatttaataa agcagatgac aacaccgctc
     2101 attgaaatcc gtcaaattta taaatcatac ggcaatacac caattttgaa taatgtttcg
     2161 ctaaacgtca atcacggtga atttttaacc ttactgggcc cctcaggttg tggaaaaaca
     2221 acccttcttc ggcttatatc tggttttgaa caaccaacgc aaggtgagat tttcattaat
     2281 ggccaatgtg ttaatcagtt accacctcaa aaacgggatg tacacacggt ctttcaaagt
     2341 tatgcccttt tcccccattt atccgttttc gaaaatgtcg catttgcctt gcgctgcaaa
     2401 aaaacgtcca accaggaaat caaggagcga gtctttgatg cgttgaaact tgtacaactt
     2461 gaatcgttag ctgatagaaa tgtgaagcag ttaagtggcg gccaacaaca acgtattgcg
     2521 attgcacgag caatcatcaa cagacctcaa gtactgttgt tagatgagcc attgagttcc
     2581 cttgactata ggcttaggaa agccatgcaa tccgaattga agcaattaca aaaaaccttg
     2641 aatatgacgt ttatttttgt aacgcatgat caggaagaag ccttatcaat gtctgaccgt
     2701 attgccgtct ttaatcatgg ccacattgaa caaattggaa cgcctaaggc tgtctacgaa
     2761 acccctgcca atctgcatgt tgccatgttt attggcgaag cgaatatatt tgatattcag
     2821 gttcacacag taaaagatca agacatcgta acgaacattg aaggaatcca actatcctgt
     2881 aaaaacactg gcaattatca agtcaacgac cggcttcatt tgatcgttcg tccagaggat
     2941 attcgagttt ggagcttatc cgaagtagaa aaaagagaag gaatgttgcc tggtcgtatt
     3001 gttgatatca tttataaagg ctctacggtt gatctcaaag tggaattgtc atcaggcaaa
     3061 atcatcaatg cttcagagtt ttttgatgaa gacgatgata agctggaata cactctccat
     3121 gaaaccgttt gggtacaatg gctgcctggc tgggaggttt tgttacctta tgaaggctaa
     3181 atcatctgca ctgtatttat tgtatttttg gttaattatt tttggcttca ttcccttgtt
     3241 gatggtgctg gttgccagct ttttatcaca agacagtatt catcttgtta ccttgccatt
     3301 gacattggaa aattattttg acttgtttac tcctgttttt gcaaaaatcc ttttcagatc
     3361 cttactgata gcctgtgtca ccaccctgct ttgtctgctc atcgcatacc cctttagcta
     3421 tctaatgatt aaatcaaaac accaatctat tttattgctg ttgattatca tacctttctg
     3481 gacaagctca ttgataagaa cttatgcttt gattgctatc cttaaataca aaggcgtgat
     3541 aaatgcccta ttgctgaaat tacacttgat tgatgttccc ttgtcactac tttataccaa
     3601 ttttgcggtc gttattggct tggtttacaa tcttttccct ttcatggttc taccgatttt
     3661 tactaatatg gaacggttcg atttcaggtt aattgaggca gctaaagatt taggggcaaa
     3721 caagtggtct atatttacac gagtattttt gcctaacaca gcccctggag tcttatcagg
     3781 ttgtttgctg gtcttgttac cagcaatgac tttattttac attcctaatg tattaggtgg
     3841 cgctcgttcc attttattgg gaaatttaat ccaaaatcaa ttccttgtcc tgagcgattg
     3901 gcctcaggga gccgctacca gtgtgctatt aacttccgtt ctgcttattt taatgttcat
     3961 ttttcggcga cagaacaatg aggtcattcg atgaaaacaa tgacacagcg cctgtttctt
     4021 tctttagttt atgcttttct ttatatcccc attgcaatac tggtgctcta ttcagtaaat
     4081 gatgccaaat tttcattaca atggcatggg ttttccttgc gatggtacaa agaactattt
     4141 catgatcggg gcttgtggtc tgctgttttc aattccatca ccctgggtct aacatcctct
     4201 cttatcgcta caacaatggg gctattggct tcaatcaact tatttttatt tcgtacaaga
     4261 caccgccaaa ttctgtatac cttattacta ttgcttatta ttattcctga catagtcctg
     4321 ggtgtcgcct tattaatttt ctttaatatt acccaagtac cgcttggctt cttgagcctt
     4381 ttagtcgccc atattacatt ctccattcct tttgttatct tgaccattaa ttcacgaata
     4441 aacactttaa atcccaacat ttattttggc gccctcgatc ttggcgccag tcgttatttt
     4501 gctttaacga gagtgttgct tcctttattg tggcctgcgg ttttaagcgc ttttttatta
     4561 agtttcactt tgtcatttga tgacgtcatt atcagctatt ttgtggcagg ccctgatttt
     4621 actatcctgc ctttgaccat ctactcttta gtccgtgctg gtgtcacgcc tgaactcaat
     4681 gcattgtgtt caattacgtt cggtttatcg atgattctcg ttattatctc tcatcgcttg
     4741 tcaggtaaat tgatatgaaa attttttcat tgctgctcgc tcttcttaca tttaaggcat
     4801 tctccaatcc agtagtcaat gtctacgcct ggggaggtga aatccctaaa aaaataattc
     4861 atcaattcga acaagagtca ggaattaaag tcaatttttc gacttatgat agcaatgaaa
     4921 ccatgtatgc caagctaaga gccagtcaaa agagcattta tgatgtcatc ctcccttccg
     4981 cttattttgt agagcgcatg caaaaacaag gcatgttgca attactcgat cataaacagc
     5041 tttcgaatct tcataatctg gatgaacaat ttgttaaaaa tgattatgat ccgggtaacc
     5101 gttatagtat acccatcatc tggggagcaa ccgggatttt ctttaatcat aattccgtat
     5161 cgaacccgcc aaaaagctgg caaggcttat gggataaaaa gtggcgcaat cagctaatgt
     5221 tacttgatga ttcacgcgaa atatttgcca tagctttgat gagtttaggg tttgatccca
     5281 atgataccaa tccaaaccac ataaaagcag cctatgaaca cttattgcaa ttggtaccta
     5341 atattaagtt atttgccagc gatagtattc aagcaatcat gatagatgag gatgctttta
     5401 ttggatcagc atggaatggc gatgccttca aagcacatac agaaaacaaa gctatcgagt
     5461 ttatttatcc tcaagatggt tttgtgattt gggtggattg tttggctatc cctaaaaacc
     5521 ccccacacct gaaagaggcg tatcaattca ttaattttat tctgaaaccg gaaacaagcg
     5581 ctcaaattgc actaaaggaa ggacatgcga ttaccaatgc cgcaggaaga gctttattgc
     5641 cattacctat aaaaaataac cctgtggttt atccgccaga taaaacacta aagaagggtt
     5701 atttccaacg cgacatcggc gaagaaacac tagcgcttta taatcaatat tgggaacagt
     5761 taaaacttac tttttagttt tctgttgtga agtagtgcta aatatacaga tgaagtactt
     5821 cattaactca acgaattata attcagaaag caagattga
//
LOCUS       DASLOE010000174         5747 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.175, whole genome shotgun sequence.
ACCESSION   DASLOE010000174 DASLOE010000000
VERSION     DASLOE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.175"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(104..1021)
                     /gene="secF"
                     /locus_tag="U6309_002535"
     CDS             complement(104..1021)
                     /gene="secF"
                     /locus_tag="U6309_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEN5505962.1"
                     /translation="MEFFNPNSKIDFMGARRWTALFSILIFVVSIGALMINGLKWGLD
                     FTGGTQIEISYSKAADLPLIRENLNKAGFKEAQVVSYGTSKDVLISIAPRADKQQSML
                     VDQVMEVLPGAVKQRVDFVGPQVGQELATKGALAIIVSLLATMIYIAMRFEYKLAVSS
                     AVALIHDPVLILGVFALFGIEFDLKALAGLLAVIGYSLNDTIVVFDRVRENFVKIRRT
                     SPIDIMNISINQTLSRTIMTSVLTLFVVVALFVYGGEAIYGFSLALIIGIVIGTYSSI
                     YVAGALAVAMGLDNKDFLPSHRREVDDRP"
     gene            complement(1034..2890)
                     /gene="secD"
                     /locus_tag="U6309_002536"
     CDS             complement(1034..2890)
                     /gene="secD"
                     /locus_tag="U6309_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEN5505963.1"
                     /translation="MQNKYPLWKNLMLIVIAVIGFIYAIPNLYTENPVVQISAGTDED
                     TETIKNQVESILKNANMDYKSLVVERDGVEVVFSSTDSQLLARDVIKNSLGSDYTVAL
                     NLAPSTPAWLEAIHADPMKQGLDLRGGVHFLLEVDVESVISRRYEGIMKNIGQELREA
                     GIRYSGIRYLSDKGIELRFRDSDSMENALVELRQKISDVVFVKTKTQNSILGAISPAE
                     LNSIRQNTIEQTMSILRNRVNELGVGEAVVQQQGATRVAVDLPGIQDAARAKQILGGT
                     ATLEFHLVDQENDPFIAKQTGVVPVNDKLYMMDGHPILLKRQVVLSGDSITSAVSSFD
                     QQTASPSVQIQLGGGGESLFTKVTRENIGKRMAIVFVETKTTTQTINGEEKRVTKKEE
                     RVISAPVIQNALGSNFQITGLTDSKEASNLALLLRAGALPAAIYPVEERTVGPTLGKE
                     NIRRGLISLEVGMGLILVLMLVYYHFFGLIADIALMLNLVFLCALLSIIDATLTLPGI
                     AGIVLTVGMAVDANVLIYERIREELRNGLSPQAAIHAGYERAFSTILDANVTTLIVAI
                     ILFAVGTGPVRGFAVVLSLGLLTSMLTGITYTRAIVNWYYGGRNVKKLSIGI"
     gene            complement(2912..3247)
                     /gene="yajC"
                     /locus_tag="U6309_002537"
     CDS             complement(2912..3247)
                     /gene="yajC"
                     /locus_tag="U6309_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEN5505964.1"
                     /translation="MSFFISDALAAAGNAPAAQTDGTFSLIMIVAIFVLFYFMIIRPQ
                     SKRAKEHRELINRLKKGDEIVTSGGILAKVVSLDDQYIKVSPAEGIEINLQKGAVTAV
                     LPKGTIKSL"
     gene            complement(3393..4226)
                     /locus_tag="U6309_002538"
     CDS             complement(3393..4226)
                     /locus_tag="U6309_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuine tRNA-ribosyltransferase"
                     /protein_id="HEN5505965.1"
                     /translation="MLSTNQNFVPVLNTEAGLCLTAANWQEIKITIASCYLDLLLLKP
                     GYSLLKNITDFTKYLGWSGHLILNASRLVSDKNGMVILISPYDGSRIKLTNSELVHLI
                     LHIKPAAVLLPETLMNGFSGLWEQWDDTILPFLSAKQLETLQVPGKHGVYFNFSQAKY
                     HDDFLSQLQKWSELPLYVSGPIGPDLIEDLNRKKSILIESDEPAKNAMRGIVYGREGN
                     VDLTDESQAFNFQLIDENCSCPTCSAQLTRAYLHHLLANTPLLCQRFLIQHNAYYVQG
                     N"
     gene            complement(4273..5286)
                     /gene="queA"
                     /locus_tag="U6309_002539"
     CDS             complement(4273..5286)
                     /gene="queA"
                     /locus_tag="U6309_002539"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEN5505966.1"
                     /translation="MNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFR
                     EIADFLQPGDLLVMNDSKVIPARLYGHKATGGKVELLVERITGDFTFLAHIKASKSLK
                     SNDLIYLDAGKRLEVLQKQDDLFLCEASENILDLLNDLGHIPLPPYIAREDESLDKER
                     YQTVYAKCAGSVAAPTAGLHFDDAVLSSIRARGVNIAYVTLHVGAGTFRPVRCERIQD
                     HKMHSEWFTVSPDLCAAVKAAKSMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIF
                     IYPGYQFKVCDGLITNFHLPESTLVMLVSAFIGHQECMALYQEAIDKRYRFFSYGDAS
                     LLL"
     gene            complement(5402..>5747)
                     /locus_tag="U6309_002540"
     CDS             complement(5402..>5747)
                     /locus_tag="U6309_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505967.1"
                     /translation="RTFKKILAIRGAKLLVPLGIASWLIYNFDYLFIWVIYYIREVLQ
                     AVLAFLTRIMPFKPYSTSIALIILLTTVSVGPVLLLDLIYRKRTYKGYAYPYLTSTLI
                     LIFCTLILLVVS"
BASE COUNT         1846 a         1292 c          957 g         1652 t
ORIGIN      
        1 ttcgtccata atccgatgtg gtttggagta aactataatc tgagacgcga ccatcaggga
       61 gcggataata agcaggtaaa atacggataa taagacccca acatcaaggt ctatcatcca
      121 cctccctgcg atgactgggt agaaaatcct tgttatccaa acccatggct actgctaaag
      181 cgccagcaac ataaattgaa gagtaagtac ctattactat tcctataatc aatgccaatg
      241 aaaacccata tatcgcctca cccccataga caaaaagcgc aaccacaaca aacagcgtca
      301 acacagatgt catgatcgtt cttgagagag tttgattgat tgaaatattc ataatatcta
      361 ttggacttgt tcggcgaatt tttacaaaat tttctcgaac acgatcaaag acaacaatgg
      421 tatcattcaa ggaataacca ataacggcca acaacccagc aagagccttc agatcaaatt
      481 caattccgaa caaagcaaaa acgcccagaa tcaatactgg atcatgaatc aaagccactg
      541 ctgaacttac agcgagttta tattcaaacc gcatagcgat atagatcatc gtggctaata
      601 aggacacaat aatcgcaaga gcccctttcg ttgctaattc ttgcccaact tgcggaccaa
      661 caaaatcaac tcgttgtttt actgctccag gcagtacttc catcacctgg tctaccagca
      721 tgctctgctg tttatccgct ctcggtgcaa tgctgataag tacatctttt gatgtaccat
      781 aactaaccac ttgagcttct ttaaaacccg ctttattcag attttctcta attaaaggta
      841 aatcagccgc tttggagtaa ctgatttcaa tttgcgttcc accagtaaaa tccaatcccc
      901 atttcaaacc attaatcatc aatgcgccaa tagacactac aaaaattaat atagaaaaca
      961 atgcagtcca acggcgcgcg cccataaaat ctatttttga atttggatta aaaaattcca
     1021 tctgagtctc gccttatatg cctatagata attttttgac attacgccca ccataatacc
     1081 aattcacaat agctctggtg taagtaatac cagttaacat agaagttaac aaacctagag
     1141 aaaggacaac cgcaaacccg cgcaccggtc ctgtgcccac cgcgaataaa atgatagcta
     1201 caattaaagt agtcacgttg gcatccaata tagttgaaaa tgctcgctca tatcctgcat
     1261 gaatagcagc ttgaggagac agtccattgc gcaactcctc tctaatccgc tcataaatca
     1321 ggacattggc atcaacagcc atgcccacgg tcaaaacaat ccccgcaatt cccggtaaag
     1381 tcagagtcgc atcaattatg gataacaatg cgcaaagaaa aactaaattc agcatcaatg
     1441 caatatcagc aattaaacca aaaaaatgat aatacacgag cattagtacc aaaattaaac
     1501 ccataccaac ttccagggaa ataagtcctc ggcgaatatt ttcctttcca agagttgggc
     1561 caacggtacg ctcttcaaca ggatagattg cagcaggtaa agcaccagct cttaataata
     1621 aagccagatt acttgcttct ttgctatcag tcagtccggt aatctggaaa ttgctgccta
     1681 aagcattttg aatcactggc gcactaataa ctcgctcttc tttctttgtt actcgttttt
     1741 cctcaccatt aattgtttgt gtagtcgttt tggtttccac aaaaacgatc gccatgcgct
     1801 taccgatatt ttcacgcgta actttagtaa acaagctctc gcccccgcca cccaattgaa
     1861 tttgaacaga tggagaagcc gtttgttgat caaaactgga aactgctgac gtaatagagt
     1921 caccacttaa aacgacctgt ctctttaata agataggatg accatccatc atatacagct
     1981 tatcattgac aggaactacg ccggtttgct ttgcaatgaa agggtcattt tcctgatcaa
     2041 ccaaatgaaa ttccaaagta gcagtaccac caagtatttg ttttgctctg gccgcgtctt
     2101 gaatgcctgg caaatcaacg gcgactcgag tcgccccttg ttgttgtacg acagcctcac
     2161 caactccgag ctcgttgaca cgatttctca agatactcat tgtctgctca atagtatttt
     2221 gtcttatgga atttaattct gctggagaaa tggcgcctaa aattgaattt tgtgttttag
     2281 ttttaacaaa aaccacatca gatatttttt gtcttaactc aactaaagca ttttccattg
     2341 agtcagaatc tctgaaacgc aattcaatcc ctttatctga taaataacgt atgcctgaat
     2401 aacgaatacc tgcttcacgt aattcttggc caatattttt cattattcct tcataacgcc
     2461 gacttataac actctcgaca tccacttcaa gaagaaaatg aacaccgcct cttaagtcca
     2521 acccttgctt cattgggtca gcatgaattg cttcaagcca agctggtgtg gaaggcgcta
     2581 aattcaaagc cactgtgtaa tcagatccca ggctattctt gataacatca cgagctaata
     2641 attgagaatc agtagaagaa aatactacct ctaccccatc cctctccaca accagggatt
     2701 tataatccat attggcattt tttaatatgg actctacctg attttttatt gtctctgtat
     2761 cttcatctgt acccgcagaa atttgaacaa cgggattttc cgtatacaaa ttcggtatag
     2821 cgtaaataaa tccaatgact gcaatcacta tcagcattag atttttccat agaggatatt
     2881 tattttgcat ttctgattcc ctatgcctaa cttaaagtga tttgatagtg ccttttggta
     2941 atactgcagt aactgcgcct ttttgcaaat taatttctat accttctgct gggcttactt
     3001 tgatgtattg atcatccagg ctcacaactt ttgccaaaat ccctccggat gttactattt
     3061 catccccctt ttttaagcga tttatcaatt ctctatgctc tttggctctt ttgctttgcg
     3121 gccttattat cataaaataa aacaagacaa aaatcgcaac gatcataatc agtgaaaaag
     3181 ttccatcagt ttgagcagct ggcgcatttc ctgctgcagc taatgcatca ctaataaaaa
     3241 aactcataaa atctcctcaa atcgggtatt aatttcctgg ttttctttcg aagatggtcg
     3301 ataaaccaat ttcaaaagat agttctaaga actcttgtat ggattaccag cgtgacatca
     3361 tatagcgaat ttactcgaag gtaaatgaat agttaattac cttgaacgta atacgcatta
     3421 tgttgaatca agaatctctg acatagcaat ggggtattag ctaacaaatg atgcagataa
     3481 gctcttgtca actgtgctga acaagtagga catgagcaat tctcatcaat cagctgaaaa
     3541 ttgaaagcct ggctttcatc cgttaaatca acattccctt cgcggccgta tacaatgccc
     3601 cgcatggcat tttttgcagg ctcatccgat tcgattaaaa tcgatttctt cctgtttaaa
     3661 tcctcgatta aatcaggacc gataggacca gagacataaa gaggtaattc agaccatttt
     3721 tgcaattgac tcaagaaatc atcatgatat tttgcttgac tgaaattgaa atacacacca
     3781 tgttttccag gcacctgcaa ggtttcaagt tgcttcgctg acaaaaaagg caatatagta
     3841 tcatcccatt gctcccatag cccggaaaaa ccattcataa gcgtttcagg taaaagcact
     3901 gcggcgggct taatatgcaa aatcaaatgc actaactctg agttagttaa tttgattcga
     3961 cttccgtcat aaggtgaaat taatatcacc atcccgtttt tatctgatac aaggcggctc
     4021 gcattgagga tgagatggcc tgaccaccct agatatttgg tgaaatcagt aatattcttc
     4081 aataatgaat atcctggctt taatagcaat aaatccagat aacaagatgc gattgtaatc
     4141 ttaatttctt gccaattggc cgcagtaaga catagtcctg cttcagtatt taaaacagga
     4201 acaaaatttt gatttgtact caacatccac tatcctctaa ctgtagccac cattattaca
     4261 ttccggaaaa ctttataaca ataaactggc atctccataa ctaaaaaaac gatacctctt
     4321 atcgattgct tcttgataaa gagccataca ctcctggtgt ccaataaaag ccgacaccaa
     4381 catcaccaga gttgattcag gcaaatgaaa attagttatt aatccatcac aaactttaaa
     4441 ctgatagcca ggataaatga aaatatcggt atcacgacta catggaatta gctctcctcc
     4501 catagcagcg ctttcaaggc tgcgtaatgc agtagtccca acagcaatta ctctatttcc
     4561 catagatttg gcagccttca ctgcagcaca cagatcagga ctaaccgtaa accactcact
     4621 atgcatttta tgatcctgaa tgcgttcaca gcgaaccggc ctgaatgttc ctgcccctac
     4681 atgaagagtg acataagcaa tattaactcc tcgcgctcta atactggata aaacagcatc
     4741 atcaaaatgc aatcctgctg taggcgcagc aacagacccc gcacacttgg catagacggt
     4801 ttgatatctc tccttatcca gtgattcatc ttctctggca atataaggtg gtaaaggaat
     4861 atgcccgaga tcatttaaaa gatctaaaat attttcgctc gcctcacaaa gaaataaatc
     4921 atcctgcttc tgtaatactt caagccgctt accggcatca agataaatga gatcatttga
     4981 ttttaatgat ttgctcgctt tgatatgggc caggaaagta aagtcgccag ttatcctctc
     5041 tactaacagt tcaaccttgc cgccagtagc tttgtggccg tataagcgtg ctggtatcac
     5101 tttactgtca ttcattacca ataggtcccc tggctgtaag aaatctgcaa tttcccggaa
     5161 ttgataatgg ccatattctt ctgtctgtcg attataaatt aaaagccgtg aatcacttct
     5221 attcgccaga ggatattggg ctattaattc ggaaggcaaa tcaaaataaa aatcttgctt
     5281 attcatagaa caacctaaaa acaaattggt gcaattatac accgaacttt agtcttaaaa
     5341 tcaattatca ggaatttatt cattttatta gaaaattaac atcatttgtt ttcttatctg
     5401 tttaagagac tacgagcaag atcagcgtac agaaaattaa aatcaaggta cttgttaaat
     5461 agggataagc ataacccttg taagttcgtt tgcgatagat caggtccagt aataatacag
     5521 gtcccactga aacagtagtg agtagtataa tgagtgctat cgatgtggaa tatggcttaa
     5581 aaggcataat ccgcgtcaaa aaagctaaca cagcttgcaa tacttctcga atataataaa
     5641 ttacccagat aaataaataa tcaaaattat aaattagcca ggaagcgatg cctaaaggaa
     5701 cgagtaactt agcccctctg attgccaaaa tttttttaaa ggttcta
//
LOCUS       DASLOE010000175         5714 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.176, whole genome shotgun sequence.
ACCESSION   DASLOE010000175 DASLOE010000000
VERSION     DASLOE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.176"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..834
                     /locus_tag="U6309_002541"
     CDS             <1..834
                     /locus_tag="U6309_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HEN5505968.1"
                     /translation="TLIIKIANDDERIKIVIMNGSRASPSAKKDIFQDYDIVYLVTEI
                     ESFVHDKNWINQFGELLIMQTPDEMDGQWPKFKGKYTYLMQFKDWNRIDLTLLHIDQL
                     EIMPRDSQSLLLLDKDNLIAPFGEPTDEDYLPSPPTEKDFVNCCNEFLWVSTYVAKGL
                     WRKQLIYTKHVSEQIVKEELIKMLMWYAGIQTNFSQPMGTYGKYIENHLELELRQKFL
                     KTYVDADYKNMWASLFKMCEIFNDLAIKISSYFGYSFNQKEFDNVVDYLQDVKNNKIP
                     P"
     gene            967..2763
                     /locus_tag="U6309_002542"
     CDS             967..2763
                     /locus_tag="U6309_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN5505969.1"
                     /translation="MPKTPNNCSLSSGNKPPTFSRILFSYMAEYRWSTAFFILFGLFW
                     AFTLPYMSYLLGQIIDEIKFQNPEQVDIFSLVLTSLILYVAIHVLRSLGYYVHGLFSL
                     TSIPEKKAKLIKQLFTHLGNQSIGYFEDKHSGYLTNKITNASSSIEPLIFNLFTIIYP
                     QSLAIILTGILLSLVIPYFGVVLWLWGATLIIYSYHSAKIGNDKAMLLANSNSKVNGK
                     VVDVVNNIHTVIYNAEMSFELGNLDTDIQDMVQNDRNLQRHMTKVMLVQHIAMNILVA
                     FFLIGSVLGYNYGYVSVGDIVFVMTSVTAIAGLTSSLGKCFLDFVTNTGRLNEGLSLL
                     GDQHTITDNANAQAHQINNGEIQFHDISFAYPNQPPIFLNFNLTIPAKQKIGIVGSSG
                     AGKTTLFKLLMRLYNVEKGVIAIDDLPINAYTKKSLCSQIAIVPQHLTLFHRSIYDNI
                     TYGCNSFDKSDVILAAKKAHCHEFIAELPEQYDTIIGEQGVKLSGGQRQRIAFARAIL
                     KNAPILLLDEATSALDSRTEQAIQDGLQNLLADKTALVIAHRLSTLKAMDRILVLEDG
                     KIIENGTHTELLEKRGAYYNYWSHQSKGFIRA"
     gene            3051..>5714
                     /locus_tag="U6309_002543"
     CDS             3051..>5714
                     /locus_tag="U6309_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent ubiquitin ligase"
                     /protein_id="HEN5505970.1"
                     /translation="MPKYVEGVELTQEGMHAIFARMGHGDITSGSIYNGVPTIDTDAL
                     NRQGFMPVLTGVGPRRDSGHWIMLIKGPGNQYFLFDPLGKTSGEGYKNTLLAQLPIAS
                     TLSVIPNNPGLNMGLCGYWVASVGLKARAELNKDNPPDLETLGRTTTEEMRNELTDNG
                     YLKITGWLRAVSDKFPAGDPQPDAKTLRETTEKDLHIEIPSPVPPVKDTAPKEVSTKP
                     TAPQVAPKHSLDSKLLENDEDVLDTIKYVHKEYLGKPYPGPLKNPKAPEEGRLPPNEG
                     PDRGPHGLAHTVRTMACAEVMIEEARKAQLRGETLGKAKNGQTLADVTPEELKKILIA
                     QAFFVVGRDDERSGYDDVHKRNFYAEYHEKSEQAFRKYVEDNKLIGKIFKDQKEVDFY
                     AAIILDKNHEWDASPAHILINQGHMVDLMRTKAPAEVALERTYNTLKYTLGSKGAEVV
                     LKAHRDFFFVTGAVVPLVNPEAIDDPSRGGPYENPYSGEKFVIVDDKVPASKKDLPKA
                     VNRDYKLKDNERFLTIKEYYAFPDVQQTYPGYKTRLEGSSYYFPTPFAGECEHDPAKC
                     LGAIQKARSKLQTDAIKNGFQSSSDKERRQPNMDEIAAARIIQQIMANPDCIGNDHVS
                     INGQELGEKFFRDLLAKCDMAVVGSLLNDTDIKNIDTLMRHEKDTEFHSTDPQAVPVK
                     IGDAWENRIRTKGGDVTQMKHDLIFLMQNDAWYFSRVNAIAQNRDKGSNFKEVLFTAL
                     MTPLTNKSLMDTSHVPAPKKLYRGLNLPQEFTNKLINQANAIIANTENTLFTDLSAEG
                     FKQIKLNDFSQMSGRTCASTTKNMKLLTDIWGSNVIFEMLDPDGLLHPKQVGTHMAGS
                     EDEFSVYLPEDVALVPTKVTLEGKTDTGEDRY"
BASE COUNT         1907 a         1108 c         1087 g         1612 t
ORIGIN      
        1 acactcatca tcaaaatagc taatgacgat gagaggatta aaattgtgat tatgaatggc
       61 tctcgagcaa gcccttcagc gaaaaaagat atttttcagg attatgatat tgtgtatctg
      121 gtaactgaaa tagaatcatt tgtccatgat aaaaattgga ttaatcagtt tggtgaatta
      181 cttatcatgc aaacacctga tgaaatggat gggcaatggc caaaatttaa aggtaaatat
      241 acttatctga tgcaatttaa agattggaac aggattgatt taaccttgtt gcacatcgat
      301 caattggaaa tcatgcctag agacagtcaa agtctcttat tgcttgataa agacaattta
      361 attgcacctt ttggcgaacc tactgatgaa gattacttgc caagcccccc tacagaaaaa
      421 gattttgtta actgttgtaa tgaattctta tgggtaagca cttacgttgc caaaggactc
      481 tggagaaagc aactcatcta cacaaaacac gtttcagagc agatagttaa agaagagtta
      541 attaaaatgc tcatgtggta tgcaggaatt caaaccaatt ttagtcaacc gatgggaact
      601 tatggaaaat atattgaaaa ccaccttgag cttgagctcc ggcaaaaatt cctcaaaacc
      661 tacgttgatg ctgattataa gaacatgtgg gcttcattat tcaaaatgtg tgaaatattc
      721 aatgacctag cgataaaaat ttcaagttat tttggttact catttaatca gaaagaattc
      781 gataatgtgg tagattattt gcaagatgta aaaaacaata aaattccacc ttaatcgaac
      841 aaatccactt ggaattaaag aataaatact attgagatat ctatattggt tatcgatttt
      901 tgcaaatgaa ttgagagcac tccgccatat gaggtggaac aaattttaaa gcttgaagga
      961 aatgttatgc ccaaaactcc aaacaattgt agtttatcca gcggtaataa accaccaacc
     1021 ttctcaagaa tcttgttctc atatatggct gaataccgct ggagtacagc attttttatt
     1081 ttatttggcc ttttttgggc atttactctg ccttatatgt cctacttact tgggcaaatc
     1141 atagatgaaa taaaatttca aaatcctgaa caggttgaca tctttagctt agtcctaacc
     1201 tcattaattc tttatgttgc aattcatgtt ttgcgaagct tgggatatta tgttcatggt
     1261 ttgttttcac ttaccagtat tcccgaaaaa aaagccaaac tgattaaaca actatttact
     1321 cacctgggca atcaatcgat cggttatttt gaagataagc actccggtta tttaacaaac
     1381 aaaattacca atgccagttc tagtattgag ccacttattt ttaatttatt taccattatt
     1441 tacccacaat cattagcaat tatcctaaca ggtatattat tgtctttagt cattccgtat
     1501 tttggagtgg ttctctggct ctggggagct acacttatta tttattcata ccattccgcc
     1561 aaaattggta atgataaagc aatgctcctt gcgaattcca atagtaaagt caacggtaag
     1621 gtagtcgatg tggtcaataa catccacaca gtaatctaca atgctgaaat gtcttttgaa
     1681 cttggtaatc tggatacaga cattcaggat atggttcaaa atgatcgcaa tttgcagcgc
     1741 cacatgacca aagtcatgct ggtacagcac attgcaatga atatcctcgt cgctttcttt
     1801 ttaataggct cagtacttgg gtataattat ggttatgtca gtgtaggcga cattgttttt
     1861 gttatgacat cagttactgc tattgcaggc ctcacaagca gccttgggaa atgctttctc
     1921 gattttgtta ctaataccgg gcgtttaaat gaagggttga gcttattggg agatcaacat
     1981 acgattactg ataacgcaaa tgctcaagca catcaaatta ataacggtga gattcaattt
     2041 catgacattt catttgctta tcccaatcaa ccgccaattt ttctaaattt taatttaacg
     2101 attccagcaa agcagaaaat aggaattgta ggtagttcag gggctggtaa aaccacatta
     2161 ttcaaattat tgatgcgatt atacaacgta gaaaaaggtg tcattgcaat agatgacttg
     2221 ccaataaatg cctacactaa aaaatcactt tgctctcaaa ttgcaatagt gccacagcat
     2281 ttaacacttt ttcatcgtag catttatgat aatatcacct acggttgcaa tagttttgat
     2341 aaatcagatg tgattttagc tgcgaagaaa gcacactgcc atgaatttat cgctgaactg
     2401 cctgagcagt atgacaccat tattggcgag caaggcgtaa aattatcagg tggacaaaga
     2461 cagcgtattg catttgcccg ggctattctc aaaaatgcac ctatcttgtt acttgatgaa
     2521 gcgacatcag ccttagactc caggactgaa caggcaattc aagatggctt acaaaatttg
     2581 ttagcagata aaactgcttt agtgatcgcg caccgtctat caacgctcaa agcaatggat
     2641 agaattctag ttttagaaga tggcaaaatc atcgagaatg gaacacatac cgaattattg
     2701 gaaaaaagag gggcgtatta taactactgg tcacaccaat cgaaaggttt catacgagca
     2761 tgaaacaatc tgctaccagc ctgtaaactg taaatataat cctaagaaaa acttataaat
     2821 attagagctt taaactctta tatcatcgta ttaattcacc tcgcttattt ttgaaacaga
     2881 aaatcccaac tgtctttcaa tttcacgaac acaaaaaatc aagagcctga ttttaaactc
     2941 ttaatatttc cttaaaacta ttattctata ataaatatac caagtgtgaa tattcgtatc
     3001 cattagaatc agtatttgca tgtacaaaca tcagtttggg agaaagtaaa atgcctaaat
     3061 acgtagaagg ggtagaatta actcaagaag gtatgcatgc tatttttgct cgtatggggc
     3121 atggcgatat taccagcggt agcatttata atggagtacc gactattgac acagatgccc
     3181 tgaacaggca aggctttatg cccgttttaa ccggagtagg ccccagaaga gactccggtc
     3241 actggatcat gttaatcaaa gggcctggta atcaatactt tctctttgac ccactaggta
     3301 aaacatcagg cgaaggttat aaaaatactt tattagccca attacccata gcatctactc
     3361 tttctgttat tcccaataac cccggcctaa acatggggct atgtggctat tgggttgctt
     3421 ctgttgggct aaaagctcgt gctgaactga acaaagacaa tcctcctgat ttagaaaccc
     3481 tggggcgaac cacgacagag gaaatgagaa atgaattaac cgataacggt tatctgaaaa
     3541 ttaccggctg gttaagggct gtctctgata aatttccagc aggtgatcct caacctgatg
     3601 cgaaaacatt aagagaaact accgaaaaag atttacacat agagatcccc tctcctgttc
     3661 ctccagtcaa agacacagct cctaaggaag tttctacaaa acctactgca ccacaagtag
     3721 ctcccaagca ttctttggac agtaaattat tagagaatga cgaagacgtt ctagacacca
     3781 taaagtatgt tcataaagag tatttaggaa aaccatatcc tggcccatta aaaaatccaa
     3841 aagcaccgga agaaggacgg cttcctccaa atgaagggcc cgatcgagga cctcatggtc
     3901 ttgctcatac agtaagaaca atggcttgtg ctgaggtgat gatagaagaa gcacgcaaag
     3961 ctcaactgag aggagagacg ttaggtaaag ctaaaaatgg tcagacttta gctgatgtaa
     4021 ctccagagga actgaaaaaa attctcatcg cacaagcctt ttttgtggta ggaagagatg
     4081 atgaacgttc cggctatgat gacgtacaca agagaaattt ctacgcggaa tatcatgaga
     4141 aaagtgagca agcgttcagg aaatacgttg aagataacaa gctcatcggc aaaatcttta
     4201 aagatcaaaa agaagtcgat ttctatgccg cgattattct ggataaaaat catgaatggg
     4261 atgcctcccc ggcacatatc ctgataaatc aaggccatat ggtagactta atgagaacta
     4321 aagcacctgc tgaagtcgca ttggaacgta cctacaatac gcttaaatat actttaggtt
     4381 cgaaaggtgc tgaagtcgtc ctcaaagccc atcgagattt tttctttgtt acaggcgctg
     4441 ttgttccctt agtaaacccc gaagcaattg atgaccccag tagaggtgga ccttatgaaa
     4501 atccttatag cggggagaaa tttgttattg ttgatgataa ggtaccagct tccaagaaag
     4561 acttaccaaa agctgtaaat cgtgactata aactgaaaga taacgaacga ttcctaacca
     4621 taaaggaata ctacgctttt cctgatgtgc agcaaaccta ccctggttac aaaacgcgtt
     4681 tggaaggaag ctcttactat ttcccaacac catttgcagg agaatgcgag cacgaccctg
     4741 caaaatgcct gggggctatc caaaaagccc gttcaaaact acaaacggat gccattaaaa
     4801 atggctttca atccagctct gataaagaga gaagacaacc caatatggat gaaattgcgg
     4861 ctgcccgcat cattcagcaa atcatggcca atcctgattg catcggtaac gatcatgtgt
     4921 ccattaatgg ccaggaactg ggggaaaaat tcttccgtga cttattagcg aaatgtgata
     4981 tggccgttgt aggctcgctg ctcaatgaca ccgacatcaa aaatatcgat actttaatgc
     5041 gacatgagaa agacaccgaa tttcattcga cagatcctca agcagttcca gtaaaaattg
     5101 gagacgcttg ggaaaataga ataagaacca aaggcggcga tgtcactcaa atgaagcatg
     5161 atttaatttt cctcatgcaa aatgacgcct ggtactttag tcgagtcaat gccattgcac
     5221 aaaaccggga taaaggctcc aatttcaaag aagtgctgtt taccgcctta atgacgccat
     5281 tgactaataa atcattaatg gatacatccc acgtcccagc tcctaaaaaa ctgtatcgtg
     5341 gattaaatct gccacaagaa tttaccaata aattaatcaa tcaagccaat gccattattg
     5401 ccaatacaga aaatacgctt tttactgatc tctctgctga aggcttcaaa caaatcaagt
     5461 taaatgattt tagccaaatg tctggtagaa cctgcgccag tacaaccaaa aatatgaaac
     5521 ttttaaccga tatatgggga tccaatgtca tttttgaaat gcttgacccg gatggtttac
     5581 tgcatccaaa acaggtagga actcatatgg ctgggtctga agatgaattt tccgtttatt
     5641 tgccggaaga tgtcgcttta gttccaacca aggtaactct tgaaggaaaa acagacacag
     5701 gggaagatcg gtat
//
LOCUS       DASLOE010000176         5648 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.177, whole genome shotgun sequence.
ACCESSION   DASLOE010000176 DASLOE010000000
VERSION     DASLOE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.177"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(154..1359)
                     /locus_tag="U6309_002544"
     CDS             complement(154..1359)
                     /locus_tag="U6309_002544"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEN5505971.1"
                     /translation="MIVQDSVCSELMRGCEEILPVPELEKKLQKGIPLKIKAGFDPTA
                     PDLHLGHTVLLNKLRQFQQFGHEVIFLIGDFTAMIGDPTGKNVTRMPLSQETVLENAK
                     TYQHQVFKILDPDKTTVAFNSQWLNKFNAVDLIRLAATHTVARMLERDDFNKRYTTGQ
                     PIAIHEFLYPLLQGYDSVALKADVELGGTDQKFNLLMGRELQKHYGFEPQVVMMTPLI
                     EGLDGVKKMSKSLDNYIGINETPEQMFGKIMSVSDELMWRYIDLLSFKTGKEIQQLKQ
                     SVLEGKNPRDVKIDFAKEIVARFHDQTQAEFAHNKFIERFQKGNIPEDLEELSLVIAE
                     PIALAQLLKQIDLTASTSESIRMVKQGAVKVDGDKISDPSLKLPIGKSYIIQVGKRRI
                     AKLSIQQAD"
     gene            1616..3049
                     /locus_tag="U6309_002545"
     CDS             1616..3049
                     /locus_tag="U6309_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN5505972.1"
                     /translation="MDKRPNIPKRKSGNSSKLLAFFALVFAFSLPYLLVKNFSHPKRS
                     SYADQTDLYPELSDELSRDIPAEASTEIPHEKPDIKSATKPMQTKQDQQDKPINTSHV
                     KKDNEWQIVNPHSGDSMATIFHRLGLTAQNLNDVIKNNPHAKMLTRIKPNQKLQFLIK
                     KNKLEKLIVPVNTIQTLTVYRAGKIYKTKIDSKKTTTQNRYVTATVQGSLYTTAQRFN
                     IPSKLVRQMTTIFNKEIDFSRSLRSGDQFSIIYDAYYIEDKMVGVGDILVVTYTNRGK
                     TYQAIRHTNAHGEQDYFTPQGTSFKKAFTRYPIKFSHISSTFALSRYHPILHYRRAHK
                     GIDLAAPIGTPIHATGDGVITIIDRHNGYGNMIKIKHDKTYSTIYGHLLKFQKGLSKG
                     SRVKRGQVIGYVGQTGLATGPHCHYELHVNNQPRNPTTISLPTAAPVPAREMAAFKAK
                     ASTLLARLKLFEQAQLASKGKKKVNVG"
     gene            complement(3180..3713)
                     /locus_tag="U6309_002546"
     CDS             complement(3180..3713)
                     /locus_tag="U6309_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HEN5505973.1"
                     /translation="MNGFANHLSNWLFLTFIVGIPLYAAIKKVNVFDAFIIGAKQGFD
                     VSVKLIPYLVAMMVAIGMLRASGFFSLMSQILSPALTMIGMPSELLPLALIRPFSGSA
                     STGVMAELIHKYGGDSFIAKTAATMMGSTETTFYVIAVYFGSVGIRRTRHAIPAGLLA
                     DLAGIIASVLICRYLFT"
     gene            complement(3710..4315)
                     /locus_tag="U6309_002547"
     CDS             complement(3710..4315)
                     /locus_tag="U6309_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HEN5505974.1"
                     /translation="MLNLIWLGMILISVVVGVIEGRIDEVVRAVTDSAKLGFELALGL
                     AGIMTFWLGIMSVATESGLVSILGKVLKPVLKRLFPDIPVEHPAMGAMVMNIAANMLG
                     LANAATPFGLQAMKELQSLNQNLKVATNSMCTFLAINTSSVQLIPVTAIAFLAANGST
                     NPSSVVFTSLIATTVSTLVAIVAVKQLAKLPRFKIERTDSL"
     gene            4385..5455
                     /locus_tag="U6309_002548"
     CDS             4385..5455
                     /locus_tag="U6309_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HEN5505975.1"
                     /translation="MKHTYNPFRYLIYFAFFITSLSSYSSQTTNIKEQTDTGLAPPAP
                     AITTNSAAIEEQANSSIRKLYHTLNTMPNTSMADRISQISAYFKGTKYILGSLGEGPN
                     ARYDQFPRYRVDGFDCDTYVNTVLSLALANSLESFQECLKHTRYKNGKRSYINRNHFT
                     SIDWNNYNQKRGLLKDITFSIRNEKKQPVALYANALINKPQWYNHKTIDTIRLQKQDK
                     NEQEKRLVELKAKGKTLETSLSNVPYIPFTALFSENKPNLHLFSQIPNGAVIEIIRPN
                     WDLRQQIGTELDISHLGFAIWINNELFFRQASSQYGKVVDVSLIDYLDKARSSPTIKG
                     INIQVVLPEKPVSNGCQLFDIR"
BASE COUNT         1886 a         1196 c          913 g         1653 t
ORIGIN      
        1 tcttctttat ctcttaagca ctcaaagtat ctcaaagtgc ccacacagtt tgtcttctct
       61 cttcttaatg aacccgccct cgtggcgtag ggcgtattct actcatttca acaactctgt
      121 caaatacttt tttaattttt ttaaaaaatt atctcagtct gcttgttgaa tactcagttt
      181 ggcaattcga cgttttccaa cttgaatgat ataagacttg ccaatgggta attttaggga
      241 aggatctgaa attttatccc catccacttt aacagcccct tgtttcacca ttcttataga
      301 ctctgatgtg ctggctgtca aatcaatttg ctttaataat tgtgctaaag ctataggctc
      361 tgcaataact aacgacagtt cttccaagtc ttcaggaata tttccttttt gaaatcgttc
      421 aataaacttg ttatgagcaa attcggcctg agtttgatca tgaaatcgcg caacaatttc
      481 tttggcaaaa tcaatcttaa catctctggg atttttccct tctaatacag attgttttaa
      541 ttgttgtatt tccttgcctg ttttaaagct taataaatca atatatcgcc acattaactc
      601 atcagaaact gacataattt ttccgaacat ttgctcaggt gtttcattga tcccaatgta
      661 attatcaagg gatttcgaca tcttctttac accatccaaa ccctctatca atggtgtcat
      721 catgacaact tgaggctcaa agccataatg cttctgtaat tctctaccca tcaataaatt
      781 aaatttctga tcagtaccgc caagttcaac gtcagctttt aatgccaccg aatcataacc
      841 ttgtaacagc gggtacaaaa attcgtgaat tgcgataggc tgtcctgtag tataacgttt
      901 attgaaatca tctcgttcca gcattcttgc cactgtatgt gttgcagcca gtcgaatcaa
      961 atcaaccgca ttaaatttat taagccattg tgaattgaaa gccacagtag tcttatcagg
     1021 gtcaagaatt ttaaatacct ggtgttggta ggtctttgca ttttccagaa ccgtttcctg
     1081 actcaatggc attcgggtta cattttttcc agtcggatca ccaatcatgg ctgtgaaatc
     1141 accaatcaaa aaaataacct catgaccaaa ttgctgaaac tgcctcaatt tattaagcag
     1201 tacagtatgt cccaaatgaa gatctggcgc tgttggatca aaacctgcct ttattttcaa
     1261 tgggatacct ttttgtaatt tcttctccaa ctcaggtaca ggaagaattt cctcacaccc
     1321 ccgcattaat tcggaacaaa ctgaatcttg aactatcatg tcattaaaac ccgtataaat
     1381 gattgaccta tcttctcgca gcgagtatct tagcccggtt agcaatttcc tgctatacca
     1441 aatgatacat tttgagataa tttattactc taaattgaga aaaatataac aaaatatcca
     1501 aataagagtc gatttagttg actcaaggta gcggtcactt ccaaataatg actgtgatat
     1561 attgattgaa atttaaatag cagcattttt cagatgacgg caatataggc aaaaaatgga
     1621 taaaagaccg aatataccaa aaagaaaatc tggtaactca tcaaagctat tagcgttttt
     1681 tgcgttggta tttgcctttt cgcttccgta tttgctagtg aaaaattttt cacaccctaa
     1741 acgaagcagt tatgctgacc aaactgattt ataccccgaa ctatcagacg agttatcaag
     1801 agatatacct gctgaagctt ccacggaaat tccgcatgaa aaacctgata ttaaatctgc
     1861 tacaaaacct atgcaaacaa agcaagatca gcaagacaaa ccaatcaata caagtcatgt
     1921 aaaaaaagat aatgaatggc aaatagtcaa tcctcattca ggagattcta tggcgaccat
     1981 tttccatagg ctaggactaa ccgcgcaaaa tttaaatgat gtgataaaaa ataacccgca
     2041 cgccaagatg cttacaagaa tcaaacccaa ccagaaatta caatttttaa ttaaaaaaaa
     2101 taaattggaa aaattaattg ttcctgtaaa caccattcaa acactaacag tttatcgcgc
     2161 agggaaaata tacaaaacca aaattgactc aaaaaaaaca accactcaaa atcgttatgt
     2221 gactgcaaca gtccaaggtt ctttgtatac aacagcccaa cgatttaaca tcccctcaaa
     2281 attagtccgt caaatgacca ctatctttaa caaagaaatt gatttttcca gatctttgcg
     2341 ctccggagat caattctcga taatttatga tgcttattac attgaagata aaatggttgg
     2401 agtaggagac attctggtag taacgtatac taatcgtgga aaaacttatc aagcaatacg
     2461 tcatactaac gctcatggtg agcaagacta ttttacccca caaggcacca gctttaaaaa
     2521 agccttcact cgatatccaa ttaaatttag ccatataagc tcgacttttg ctttatcgag
     2581 ataccaccca attcttcatt acagacgagc gcacaaaggt atcgatttgg ctgccccgat
     2641 tggaacacca atacatgcta ccggcgatgg ggttattaca atcatcgata ggcataacgg
     2701 atatggcaat atgattaaaa ttaagcatga taaaacatac agcactattt atgggcattt
     2761 actcaaattc cagaaaggtc tatcaaaagg cagtagagta aaacgaggtc aagtaattgg
     2821 ttatgtagga caaaccggcc tggcaaccgg acctcattgc cattatgagc ttcatgtcaa
     2881 taatcagcca agaaacccaa ctacaataag cttgcctact gcagcaccag taccagcaag
     2941 agaaatggca gcattcaaag caaaagccag tacactttta gcccgtctaa aacttttcga
     3001 gcaagcgcaa ttagcaagta aagggaaaaa gaaagttaac gttggataaa aactgtgcgt
     3061 tggatttttc cataccccaa tacctaatct aaatgacagc agacacttct ttataaagcg
     3121 agtaagctcc aagtaattca aacataacgt tactcttttt gtgttaaaaa aatcttatat
     3181 catgtaaata aatatcgaca aatcaaaacc gaggcaatga ttccagctaa atctgccaat
     3241 aatccggcag gaatagcatg tcttgttctt ctgataccca cagacccaaa ataaacggca
     3301 atcacataaa aagtagtctc tgtacttccc atcattgttg ctgcggtttt tgcgatgaat
     3361 gaatctcctc cgtatttatg aatcagctct gccataactc ctgttgaagc acttccagag
     3421 aatggtctaa taagagctaa aggtaataat tcggaaggca ttcctatcat agtcaacgct
     3481 ggcgataaga tctgactcat taaggaaaaa aaacctgaag ctcttagcat gccaatggca
     3541 accatcatcg caactaaata aggtataagc tttacgctca catcaaaacc ttgttttgct
     3601 ccaattatga aagcatcaaa tacatttact tttttaattg cagcgtacaa agggatacca
     3661 acgatgaaag tcaaaaacaa ccaattggat aaatgattag cgaatccatt cataagctgt
     3721 ctgtcctttc aattttaaat cgcggtaatt tagccaattg ttttacagca acgatagcta
     3781 ccagagtaga aacagtagtc gcgatcaaag aagtgaagac cacactactt gggtttgtgc
     3841 tcccgtttgc agcaagaaat gcgatagcgg tgactggaat taattgcaca ctggaagtgt
     3901 ttattgccaa gaaagtacac atggaattcg tggcaacttt caaattctga ttaaggctct
     3961 gcaattcttt cattgcctgc aatccaaaag gggtcgcggc attagcaaga cccaacatgt
     4021 tcgcagcaat attcattacc attgctccca ttgcaggatg ctcaacaggt atatcaggaa
     4081 ataatcgctt taacaccggt ttgagtactt tacctaaaat agaaactaaa cctgattctg
     4141 tagcaaccga cattattcct aaccagaaag tcattatccc agccaaacca agcgctaact
     4201 caaatcccag cttggcagag tcagtaactg ctcttaccac ctcgtcaata cgcccctcaa
     4261 taactccaac caccactgaa atcagtatca ttcctaacca tattagattg agcattattg
     4321 cctccaacct ctgaaaaagg ttaatatgta accaaaatta gatttaaaat accaatcagg
     4381 acttatgaaa catacttaca atcccttcag atacttaata tatttcgctt tttttatcac
     4441 cagcctttcc agttatagca gccaaacaac caacattaag gaacaaactg atacaggttt
     4501 agccccaccc gcccctgcga ttaccactaa ctcagctgct attgaagaac aggctaattc
     4561 ttcaattagg aaactatatc acacactcaa taccatgccg aacacttcta tggcagatag
     4621 aattagccag atcagcgcat actttaaagg gacaaaatat attcttggct ctttgggcga
     4681 aggtcccaat gctcgttatg accaatttcc cagatatcga gttgatggct ttgattgtga
     4741 tacctatgtc aatacagtac tttctctagc cttagctaac tccctggaat cattccagga
     4801 gtgtttaaaa catactcgct ataaaaatgg aaaaagatct tatatcaaca gaaatcattt
     4861 tacctctata gactggaata actataacca gaagagaggt ttacttaaag acattacctt
     4921 ctctatcaga aatgaaaaaa agcagcctgt tgctctttat gctaatgcct taatcaacaa
     4981 accacaatgg tataatcata aaacgatcga taccattcga ttgcaaaaac aagataaaaa
     5041 tgagcaggaa aaacgtcttg tggaactaaa ggccaaaggt aaaacgctgg aaacttctct
     5101 ttctaatgtc ccttatattc cgttcactgc cttgttttca gagaataaac ctaacctgca
     5161 tttattttcc caaattccta acggggctgt tatcgaaatt atcagaccca attgggactt
     5221 acgccaacaa atagggacag aattagatat ttcgcattta ggctttgcta tttggattaa
     5281 taacgaactg ttttttcggc aagcctcttc tcaatatggt aaagtagttg atgtatcctt
     5341 aattgattac cttgataaag cacgcagtag cccaactata aaagggatta atattcaagt
     5401 tgtattgcct gaaaaaccag tatccaatgg gtgccagctt tttgatattc gatagcttat
     5461 aaattaatat cttaatggtt aacctatagt tttgcgtaat tacgcgcgta taaccattat
     5521 ttatttcatg aaatagccag tgtttttatt aacgagatac tcgtgtttct taataaaaaa
     5581 atcgatttct attttaattg actcgcttgg gtattatcta gcagttggcg gctggaaata
     5641 tgctcaat
//
LOCUS       DASLOE010000177         5514 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.178, whole genome shotgun sequence.
ACCESSION   DASLOE010000177 DASLOE010000000
VERSION     DASLOE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.178"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            283..921
                     /locus_tag="U6309_002549"
     CDS             283..921
                     /locus_tag="U6309_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505976.1"
                     /translation="MPTYFDPIMQEDTVLDENTIVYLVKIGDNKFSIKAISSGLEHLP
                     SDPTTHAEKYWPIPAKSLIDHSSNKLLFEEDKLSNQPISKDQVIELFAVDPDKTEPKQ
                     FSDSVKRELTENWAREVLQDQFAHAGQNPHFFFYQPLFIHGHHGDNPAQQQDNAMPIP
                     LPLLMFSLIPPMNNNNQQQMTLLFFNVLLVPQDQDHEHEQENEGEHAPSNGH"
     gene            complement(1015..1710)
                     /locus_tag="U6309_002550"
     CDS             complement(1015..1710)
                     /locus_tag="U6309_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505977.1"
                     /translation="MARFFKDREIEIKDSAGEYYKKLCNALKTTMSVCIASTSALVIN
                     ELTAKINKDRMTQYEENSSVAIHGIGEIRVFLLRIYKHLEDNKIDFLDGRAAIKNTMS
                     KGMAGNCEHQAFYLAALLRQQNIPALIYDIEDIGHTVVITQNYLLDPWIGEIFALAKT
                     DLSKFFGSSLNMKASWLNRLLSNKKFSYPDELTLQTLNEYFVQQEDQYRIGLSILDNK
                     EGMDHQMSCELPY"
     gene            1843..1977
                     /locus_tag="U6309_002551"
     CDS             1843..1977
                     /locus_tag="U6309_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEN5505978.1"
                     /translation="MEFKMTKYRDAGTGRYVTKEFAKKHPGTTVGENSKPKSQRKHKK
                     "
     gene            2268..4328
                     /locus_tag="U6309_002552"
     CDS             2268..4328
                     /locus_tag="U6309_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505979.1"
                     /translation="MKFFLKQSRQHSSNQKKRNQPAQSEDHHLIIRQAQFGTISIDFF
                     GSLRFIFTFSLFCFRAIASLAISIVSGKELFDFIFNRTKIGLRSSIDTITVRRGVTNA
                     SQKIQETTQKKSLLEKESEEPSILTNKNPTIKPNTTHTQNAFFTKSEEKSKTKSFKAV
                     EFHDFNNSEVLMAFMEKINGLIAQDEAIAIPIKEDKSLYSAGEEYIEKLIEIMKSSEL
                     LKHEFWNRLNQMVHKDIQLFVSKLSPTKEKGKFGSSDLDYLWRYFNGFEFEPSKRENR
                     KDKFAVMLEALSEEQLKASFDSPDFLSLLNKDYYIETAANTLKRKQLALFAANINRHD
                     ILNLMIKKLKPSPYLLGKLVSVMPYATKKIKIALIDQIAHLPHSASFKIELAKLAEHY
                     ISMNTQAYIKRYQSLPALPSNTIQTKPPLSKWSSVTAIPKPTCTNISVAKEEWTDNAF
                     EAFLSELNATTYIINEKKIIEDLNSLPDHRIKMIAERASLPDYKDMLKHFWMIESKTI
                     NHRSFIENRKNRLKIILENLSESQLKNSIEDNKFWDIFRNTQEPYCKMAAKALSPKQF
                     EIIISNATLERHADFAVIISQIKKDSSADKERLQKIIEAIIPHTTPWFALALERQIKG
                     LLASDKSLFEKFNTDLKKYLRKSLERPGVSLKYRRDRDLNRHAISHLLTEPLENSYKA
                     SFSL"
     gene            4570..5451
                     /locus_tag="U6309_002553"
     CDS             4570..5451
                     /locus_tag="U6309_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN5505980.1"
                     /translation="MLKRVLNTAKAKQPISTTGIFAHTYYWLTSPAGDQSYQNPNYKN
                     EEDNNAGTAIYFIHGTADQPAAFKRVAERLIDAGLPDEICSLNLLAFDQRYQGKSIKF
                     FAEQLKNKIKANQHQRVILMAHSRGGLVASYFAEFLAKEISIEVPLVITVGTPFNGSY
                     LAVKPLSWFSDSIREMEIDSEFLAQLKQEIVEHSVSAYHFFIAKEDAIVPGESGYIKE
                     YVDKYSDSLHILDRHGHLSIMSSHRLVSHIAGLLHDYMDHCETDAEEVKISKLGELTL
                     IEDYYSVKSEPAPSFNP"
BASE COUNT         1898 a         1037 c          912 g         1667 t
ORIGIN      
        1 tatctgttat tcaaatgtgc ctaatacgat tgacatatag gtaaactgta agctcttgcc
       61 ttaattaaca ggtttcctga aaactagatt aatacttatt ttcaatactc aaagaataat
      121 taatagtctt tcaaaaacat tcattttgca cataactttt tctcctggtt tcaagcaatt
      181 attattttca catgatcaaa taatgaatca aaagtattgc aaaagccaat ttgatcctat
      241 aagttactat tgagtttaaa taacagctaa aggactatta ttatgccaac ttattttgat
      301 cctattatgc aagaagatac tgtgctggat gaaaacacga tcgtttacct ggttaagatt
      361 ggtgataata aattcagtat taaagcaatt tcatcaggat tggagcatct ccccagcgac
      421 ccaactactc acgcggagaa atattggcct attcctgcca aatcattgat tgatcattca
      481 agtaacaaac tcctttttga agaggataag ttaagcaatc aaccgataag caaagatcag
      541 gtaatagaac tttttgcagt tgatcccgat aagactgaac ccaagcaatt tagtgactcc
      601 gttaagagag agttaactga aaactgggca cgagaggtac tacaggatca atttgctcat
      661 gcagggcaaa atcctcattt ctttttttat caaccactat tcatccatgg gcatcatgga
      721 gataaccctg ctcaacaaca ggataatgcg atgccaattc ctctcccact cctgatgttt
      781 tccttgatac cccccatgaa taataataac caacaacaaa tgacattatt attttttaac
      841 gtactcctgg taccccagga tcaagaccat gaacatgagc aggaaaatga aggagagcat
      901 gctccttcta atgggcactg acattaaacc taacttgcat ttccaacaag attaagtcgg
      961 gcactgcgcc cgacatttct aatttaacag gtgcgctcag gcggcaatgc ttttttaata
     1021 aggtagttcg caagacattt gatgatccat accctcttta ttatctaaaa tactaagtcc
     1081 gattcgatat tggtcttctt gttgtacaaa atattcgttt aatgtttgga gagtaagttc
     1141 atcagggtag ctgaattttt tattacttaa taatcgattt agccacgagg ctttcatatt
     1201 cagtgacgac ccgaaaaatt tgctgaggtc agttttggca agtgcaaaaa tctcgcctat
     1261 ccatggatca aggagataat tttgagtgat aactacagta tggccaatgt cttctatatc
     1321 ataaataagc gcagggatat tttgttgtct cagcaaggca gcaagataaa atgcttggtg
     1381 ttcgcaattt cctgccattc cctttgacat tgtgtttttt attgccgccc tcccatccag
     1441 aaaatcaatc ttgttgtcct caagatgttt atatatcctg agtagaaaaa ctcttatttc
     1501 accgatacca tgaatagcaa cagagctatt ttcctcatat tgagtcattc tatctttatt
     1561 gatttttgca gtgagttcat taataactaa tgcagaagtg ctggcaatgc acactgacat
     1621 ggttgttttt agagcattac acagtttttt gtaatactca cctgctgaat ctttgatttc
     1681 aatttccctg tctttgaaaa atctggccat gtctaatccc ttatatccaa taagggtttt
     1741 agtttacata caagtatgag aggagcaagt agaacagcct ctttttcacc aaaactcctc
     1801 ttgcgaactg aatttgctat acttccattg gaatggataa aaatggagtt taaaatgaca
     1861 aaatatagag atgcaggaac cggtcgatac gtcaccaaag agtttgcaaa gaaacatccc
     1921 ggaacaaccg ttggtgaaaa tagcaaacca aaatcacaaa gaaaacataa aaaataatcg
     1981 ctatgaagta attagccttt ggttgcgttt gacgcaagac actaatcatg ttgtcctaaa
     2041 aataggccag agagagtttc ttgatatttt aaatcataaa aggtaaagag ttttcataaa
     2101 acaggacttg tatatcgcat ttagacattg atgttttaac ataaatccag caatataaat
     2161 cttttgcacg aattcaactc tattaacgaa ttctcaggat gatttagtta ataaaataac
     2221 ccattatttt tacattacat ttaaccatat ggatattagt taatataatg aaattttttt
     2281 taaaacaatc tcgccagcat tcctctaatc aaaaaaaaag aaaccagcct gctcaatctg
     2341 aagaccacca tttaataatt cgtcaagctc aatttgggac tatctctatt gatttttttg
     2401 gcagcctaag atttatcttc actttttcat tattttgttt tagagcgatt gctagcttgg
     2461 ctattagcat agtttccgga aaagagcttt ttgattttat atttaataga acaaaaattg
     2521 gccttagaag ttcgattgat accattacag tgagaagagg agtaacaaac gcaagtcaga
     2581 agatacaaga aacaacgcaa aaaaaatcat tattagaaaa agagtcagaa gaaccatcta
     2641 ttttgaccaa caagaatcca accatcaagc ccaatactac tcatacgcag aatgcttttt
     2701 tcacaaaatc tgaagagaaa tcaaaaacaa aatcatttaa agcggtcgaa tttcatgatt
     2761 tcaacaattc tgaagtgctt atggctttta tggaaaaaat aaatgggctt atagcacaag
     2821 acgaggcaat agctatcccg ataaaagagg ataagagcct atattcggca ggggaagagt
     2881 atattgaaaa attaattgag ataatgaaaa gctctgagtt actaaaacat gagttttgga
     2941 ataggctgaa ccagatggtt cataaagaca ttcaattatt tgtcagtaaa cttagtccaa
     3001 ctaaagaaaa agggaaattt ggttcaagtg atctggatta tttatggcgt tattttaatg
     3061 gttttgaatt tgagccttca aaaagagaaa atagaaaaga taagtttgca gtgatgcttg
     3121 aagccctttc agaagaacag ttaaaggctt cttttgattc gcctgatttt ttaagccttt
     3181 taaataaaga ctactacatt gagaccgcag ctaacacatt aaaacgtaaa caattggcat
     3241 tgtttgctgc aaatatcaac aggcacgaca ttctgaattt aatgataaaa aaactaaaac
     3301 caagccctta tttgttaggc aaacttgttt ctgttatgcc ttatgccact aaaaaaatta
     3361 aaatagccct gatcgatcag attgcccatt tacctcattc cgcttctttc aagattgaac
     3421 tcgcgaaatt ggctgaacat tatattagta tgaataccca agcatatata aaacgttacc
     3481 agtccctacc tgctctgcca tccaatacaa tccaaactaa acctcctctc tcaaaatggt
     3541 cttctgtaac tgctatcccc aaaccaacat gtaccaatat atctgtagca aaagaagagt
     3601 ggactgataa tgcgtttgaa gcattcctga gtgaactgaa tgctacaacg tacattatca
     3661 atgagaaaaa gataattgaa gatttaaata gtttgccaga tcatagaatt aaaatgatag
     3721 cagaaagggc ttctttaccc gattataagg atatgctaaa acacttttgg atgattgaat
     3781 ccaagacaat aaatcataga tctttcatag agaacagaaa aaatcgcctg aaaattattc
     3841 tagaaaattt atcggaatct caattaaaaa attccataga agacaataag ttttgggaca
     3901 tttttagaaa cactcaagag ccttattgta aaatggctgc caaagcatta tcccccaaac
     3961 aatttgaaat aatcatttct aatgccacac tcgaaaggca tgctgatttt gccgtaatca
     4021 tttctcaaat taaaaaagac agcagcgctg acaaagaaag actgcaaaaa attatagaag
     4081 caatcattcc ccatactact ccatggtttg ctttagcttt agaacgtcaa ataaaaggtt
     4141 tattggcaag tgataaatcc ttgttcgaga aatttaacac tgatttaaaa aaatacctcc
     4201 gtaaatcgct ggaaagaccc ggagttagtt taaaatacag aagagacaga gatctaaatc
     4261 gacatgctat ttctcacctc ctgacagaac cacttgaaaa ctcttacaaa gcgtccttta
     4321 gtctttaaag atactctact actaccttaa gaactctctg cttttgacca gtaaagcacc
     4381 aattacaaaa aaccaataga gacgttaacc aaatcacaat tcattcataa ttcattgaca
     4441 ataactgacc ccgataactc tttaaagcac agtttataat tgactcatat taagctatat
     4501 tattgagaaa ttattgacag tatccgactt tcggaactcg acgctccaca ataaaaaagg
     4561 aatttaacta tgttgaagcg cgttctcaat actgcaaaag ccaaacaacc catttccacc
     4621 acgggtatat ttgctcatac ttattattgg ttaacttctc cagctggcga tcaatcctat
     4681 caaaatccta attacaaaaa cgaagaagac aacaacgcag ggacggcaat ctattttatc
     4741 catggtacag ctgatcaacc agcggcattt aagcgtgttg ctgaacgttt gatcgatgcc
     4801 ggtcttcctg atgaaatttg ctctctgaat ttactagcct ttgatcagcg ttaccaggga
     4861 aaaagtatca aattttttgc tgaacaactc aaaaataaaa taaaagctaa tcagcatcag
     4921 cgagtcattt taatggcaca ttccagagga gggctggttg cttcttattt tgcagagttt
     4981 ttagcgaaag agatctccat tgaggtaccg ctagttatta cagtgggcac cccgtttaat
     5041 ggttcttacc ttgctgtaaa accattgtca tggttttctg attctattag agaaatggag
     5101 attgatagtg agtttcttgc acaacttaaa caagagatag tagaacactc cgttagtgcg
     5161 taccattttt ttattgcaaa agaagatgcg attgtacctg gagaatcagg gtatataaaa
     5221 gaatacgttg ataaatactc agattcgctg catattttag atcgccacgg ccacctttcc
     5281 ataatgtcct ctcatcgttt ggtatcccat attgccggat tactccacga ttatatggat
     5341 cactgtgaaa ctgatgcaga agaagtaaaa atttctaaat taggggagct cacactaatt
     5401 gaagactatt attctgtaaa atctgaacca gccccctctt tcaatccatg aaacgtccat
     5461 tcatggattt ttggttatta tttattcaga aaaataaact acattggtaa ttag
//
LOCUS       DASLOE010000178         5508 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.179, whole genome shotgun sequence.
ACCESSION   DASLOE010000178 DASLOE010000000
VERSION     DASLOE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.179"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..893
                     /locus_tag="U6309_002554"
     CDS             <1..893
                     /locus_tag="U6309_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505981.1"
                     /translation="CNDPNYQFPTHLPPHIKFLLKFVLNKWCFLHQKRKLIRLPLKQL
                     VAMDLLDYSPKLPWVTNSGEADIVMVESNFAKDLFKKEGLKNKRIKVLGNLKFDAMNT
                     ILNDLKSYKAYIYEKYNLCRDRNLILCAFPSYLPERKLLDFNSYEDLIYKYVLGLQAS
                     NHHVLYSIHPSGIPWVGDKLRHLGQLVAEEKIFNLIPICDIFLATVSSTIKWARACGK
                     LVLNYDCFGFNYDVYKKDSAVIHINSFDELLVWVDKLNNQQVSDILMELSPFIPSNYW
                     GKIDGQSFHKIRYYIDALAK"
     gene            complement(1239..2314)
                     /locus_tag="U6309_002555"
     CDS             complement(join(1239..2065,2065..2314))
                     /locus_tag="U6309_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611486.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN5505982.1"
                     /translation="MKKRYSEEQIIKAIKSHESGVKVEEICRDYSISSGTFYNWRSKY
                     AGFEVNEAKRLRDLESENTKLKKLLADKLLELEAMKDVLFKKVVKPAAKKPIVSHLIS
                     KFKLSECAACRLAGLSRISFRYQSKSRTDEALRERLKELACQYPAYGYLMLHGLLKAE
                     GLVTNKKHTYRLYSEEGLQVRTKKRKKLIRPRQPIEIPATVNQRWSMDFVSDQLSNGR
                     RFGILNVVDDYSREVIGQLVSVSISGRLVARFLNNLIEERNKPTKIICDNGSEFTSKA
                     MFFWSKETNVGLGFIQPGKPTQNAFVESLNGKFRNECLNQHWFRTLEEAKYEIEKWRQ
                     HYNYVRPHSSLNYVAPVEFARQVA"
     gene            complement(2499..3527)
                     /locus_tag="U6309_002556"
     CDS             complement(2499..3527)
                     /locus_tag="U6309_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEN5505983.1"
                     /translation="MTHIKKETKIVKLTNIYSVEDEQDSYIGKRVIDMIRATKQVETP
                     PFPRQIQVETTNICNHNCEFCAYTVMERPKRQMDPDLFKRLVKEAYECGAREIGLFSG
                     AEPLTCKYLTEYIDYCRRLGYEYMYLSTNGSVPDKNKFKQILDAGLNSIKFSVNGGNR
                     AIYKQIHGNDHFERVIDNIKFVSQYRCNVKQQVYLAVSFVGMDSSKETFEELKQILDG
                     YVDEILYYDAANQSGQMPHLPLPPYRECHLPFSKLHISREGYLRACCNDYENLLAMED
                     LNITPMKEAWNSKLFQELRSKHINNKLDGILCGNCIRASKIKACAINPELIHQKDNKL
                     KQIPIIAE"
     gene            complement(3559..4485)
                     /locus_tag="U6309_002557"
                     /pseudo
     CDS             complement(3559..4485)
                     /locus_tag="U6309_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
     gene            4575..4943
                     /locus_tag="U6309_002558"
     CDS             4575..4943
                     /locus_tag="U6309_002558"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505984.1"
                     /translation="MLAPIVEIFCDIDDFCKAWFKEMSPYLLPSPNRKRQRPCRLSAS
                     EIMTVMILFHLSHYRTFKDYYRECVEMDMKSYFPKLVSYNRFVEIMSSVVTPLSAYLL
                     SHMGKKTGCQCRLKIDPFAR"
     gene            complement(4969..>5508)
                     /gene="istB"
                     /locus_tag="U6309_002559"
     CDS             complement(4969..>5508)
                     /gene="istB"
                     /locus_tag="U6309_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISLpn12 family helper ATPase
                     IstB"
                     /protein_id="HEN5505985.1"
                     /translation="KNKIRELTSLAFIERQENILLLGPSGLGKTHLAIALGYLAVQAN
                     IKTKFISAADLMLLLETAHRQGRYKDIMRRVINNARLLIIDEIGYLPMNRDQANHFFQ
                     VIANRYEKGSIIATSNLAFGQWDNTFAGDKVLTAAMLDRLLHHAHVIQCRGDSYRLKD
                     KFKAGLIQNQKEEQNNITD"
BASE COUNT         1726 a         1031 c          893 g         1858 t
ORIGIN      
        1 actgtaatga tccaaattat caatttccca ctcatctacc gcctcatatt aaatttcttt
       61 tgaaatttgt tttaaataaa tggtgttttc tgcaccaaaa aagaaaactt attcgccttc
      121 ctctgaaaca gttagttgca atggatttat tagattattc gccaaaatta ccttgggtaa
      181 caaatagtgg tgaggccgat atagttatgg ttgaaagtaa tttcgctaaa gatctattca
      241 aaaaagaagg acttaaaaat aagcgaatta aagttttggg gaatctaaaa tttgatgcca
      301 tgaatacaat attgaatgat ttgaagagct ataaggccta catatatgaa aaatataatt
      361 tatgcagaga ccgaaattta attttgtgtg cctttccttc ttacttacct gagagaaagc
      421 tactagattt taatagctat gaggatttga tttataaata cgtattagga ttacaagcat
      481 caaatcatca tgtactatat tcaattcacc catcaggaat tccttgggtt ggagataaat
      541 taaggcatct tggccaatta gttgcagaag aaaagatttt taatttaatt ccgatatgtg
      601 atattttctt ggcaacagtt tcaagcacca tcaaatgggc tagagcgtgt ggtaagcttg
      661 tattaaacta tgactgtttt ggttttaatt atgatgtata taagaaagat tctgcagtta
      721 ttcatattaa tagttttgat gagctcttag tttgggtgga taaactgaat aaccaacaag
      781 tatcagatat cctcatggag ctaagccctt ttattccatc taattattgg gggaaaattg
      841 atgggcaatc atttcataag attcgttatt acattgatgc tctagctaaa taacatcacc
      901 tagatatagg cagagctaaa ttctcaccaa gtgaatcgat ttgagataga agcagctaat
      961 tctgatgaga agttaataat aaaattatca ggactttgat atcttgtcaa ctcaatagct
     1021 gtttggacaa tcccattgta tcccaaaaaa ttgatttttg gagctcaatc agaacaaggt
     1081 agtcgtttta ttgaacgtat cttttctgtt gttgctactt ataaacagca aaaaaagatg
     1141 tcttggcatt tctcatggaa gcacttcatc aatcggcttc aattcaaaag tgacctttcc
     1201 cctgaattaa taccacaaca ttgatgagat aaattatatt aagctacctg ccttgcaaac
     1261 tccaccggcg caacatagtt aagagaacta tgaggacgta catagttata atgttggcgc
     1321 catttttcaa tctcatattt agcttcctca agcgttctaa accagtgttg attaagacat
     1381 tcatttctaa atttgccatt taaactttcg acaaaagcat tttgtgtcgg ttttccaggc
     1441 tgaataaagc ctaagccgac gtttgtttct ttgctccaaa agaacattgc cttgctagta
     1501 aattcggagc cgttgtcaca aataatcttt gttggtttat ttcgttcttc tatcaggtta
     1561 tttagaaagc gtgccaccag cctaccacta atggatactg aaactagctg accaataact
     1621 tcacgagaat aatcatccac tacatttagt attccaaacc gtcttccatt actcagttga
     1681 tctgaaacaa agtccattga ccaccgttga ttaaccgttg caggaatttc gattggctgg
     1741 cgaggacgaa tgagtttttt ccttttcttt gttctcactt gtaagccttc ttcagaatac
     1801 aaccgataag tatgtttctt atttgtgact aaaccttccg ctttaagaag tccatgaagc
     1861 attaaatacc cataagcagg atattggcag gccaattcct ttaatcgctc tctgagcgct
     1921 tcatctgtcc ttgatttgga ttgataccgg aatgatattc gactcaatcc cgccagcctg
     1981 cacgccgcac actcacttaa tttaaacttt gaaatgaggt ggctgacaat tggcttcttg
     2041 gccgctggct ttaccacttt tttgataaca catccttcat ggcttcaagt tcaagaagtt
     2101 tatcggccag tagcttctta agcttggtgt tctcactttc aagatcccgc aaacgcttag
     2161 cttcatttac ttcaaaccca gcatacttac tgcgccaatt ataaaaagta cctgaagaga
     2221 tgctgtagtc tcggcaaatt tcttcaactt tgacaccaga ttcgtgggat tttatcgctt
     2281 taattatttg ctcttcgctg tatcgctttt tcatcttgag atctcctatt tttaaatttt
     2341 atcggaaatc tcatctttgt catggtctta tttttggggg ataggtcaaa agaaacttag
     2401 acgccccttt acaaggggcg tgagcgttta cacctaacag ggcggccgag ttagatattt
     2461 aaaaatattg gataataaaa actatataaa cagaaaactt attcagcaat tataggaatc
     2521 tgttttaatt tattatcctt ttgatgaatg agctcaggat taattgcgca tgcttttatt
     2581 ttagaagccc gaatacaatt tccgcataat ataccatcca atttattatt aatatgttta
     2641 cttcttaatt cttggaaaag cttactattc catgcttctt tcataggagt aatatttaaa
     2701 tcttccattg ccaataaatt ttcataatca ttacaacaag ctcttaagta tccctctcta
     2761 ctgatatgta atttactgaa aggtaaatga cattctcgat acggaggcaa cggtaaatgt
     2821 ggcatttgcc ctgattgatt agccgcatca taataaagta tttcatctac atagccatcc
     2881 aagatctgct ttaattcttc gaatgtttct tttgatgagt ccatacccac aaaagataca
     2941 gctagataaa cttgttgttt aacgttacaa cgatattggc tgacaaattt tatattatca
     3001 attactcgct caaagtgatc attcccatga atttgtttat atatcgctct atttccccca
     3061 ttaacagaaa attttatgga atttagcccc gcatcaagta tttgcttaaa tttattctta
     3121 tcaggaactg aaccattagt ggataaatac atatactcgt atcctaatcg tctgcagtaa
     3181 tcaatatatt cagttaaata tttgcatgtg agtggttctg ctccagaaaa taaaccaatt
     3241 tctctagctc cacattcata tgcttctttt accaaccgct taaataaatc aggatccatt
     3301 tgacgcttag gtctttccat aactgtataa gcacaaaact cacaattgtg attacaaatg
     3361 tttgttgttt ctacctgtat ttgtctaggg aatggtgggg tttccacttg ctttgtggca
     3421 cgaatcatgt ctatgactcg tttacctata taactatctt gttcatcctc aacagaataa
     3481 atattagtca atttaacaat tttagtttct ttttttatat gtgtcataca aatcctttaa
     3541 aaaaaattat ataataattc agtgtaatcg caatatatca agcattatta atctcataaa
     3601 cacatttacc aaaataccac taataaaaat cttatcagct attatttttg cttttgagac
     3661 tacattttct gaataaccat tagcgaatgg attgtttact atgtttaaag caagctcatg
     3721 tttgtagtaa gaagcaacta aaaaccatgc ataagtttca attaatgttt cttctccccc
     3781 ttctccccaa gccattgcgg cagaataagc tgaaaatgct ccaggattta ctataaatcg
     3841 atatgcagca taaactctgt tatcaggaca aatactgtta ccccccattc cttcgtttac
     3901 agaagattgc ataactgcaa cattcttacc caaatcaaaa aattgatttg ctttattttt
     3961 tctttgaaca aaaaaagcac atgacaagtt aacagctata tttccactaa atagtccgaa
     4021 gggaccaaga gcataatgtg cacccctgat aaagttattt tcaataacag gatgaccact
     4081 tctggacgac ttgatccaaa ttccaaaatt tcgtccccac attggccaac gcgcaaaaat
     4141 aatatgatta ttctttatac gacaatctat tccaccatat aaagcaattc caacattttc
     4201 tccattacct gtagcatatc ctagacttat ggtattagac tcaattgaac agagattacc
     4261 ttcagaataa atccctgtag caaaagaatg tggtgaatta gagttagggc ccacaccaat
     4321 tattcgtgaa ttctttataa taaaattata tgccttgata tatattccct gcataatgga
     4381 gctttctatt gccacatttt gaataattat atttttaggt tttgattgag aaaaattgta
     4441 gatactattt gagataaaat aacctcagct cgatataaga ataaggttat gtgattgaat
     4501 tattgaaaaa attagcggtg ctatatacat tttgtttttt taaaattcac gcctgaatta
     4561 tgaaaggaaa caagatgtta gcaccgatag tagaaatatt ctgtgatatt gatgattttt
     4621 gcaaggcatg gtttaaagaa atgtcgcctt atttacttcc atcaccgaat cgcaaaagac
     4681 aacgcccttg caggctgtca gccagtgaaa tcatgacggt catgatttta tttcatctca
     4741 gccattatcg cacttttaaa gactattatc gtgaatgcgt ggagatggat atgaaatcgt
     4801 attttccgaa acttgtcagc tataatcggt tcgtagaaat catgtcatcg gtagtgacgc
     4861 ctttatcagc ctatttgctg tcgcatatgg ggaagaagac agggtgtcaa tgccgattga
     4921 aaattgatcc ttttgccaga tgaaaattga tccctcctgg taaaaaaatt aatcagttat
     4981 attattttgc tcctccttct gattttgaat taaaccggcc ttaaatttgt cttttaatct
     5041 ataactgtcg cctctgcatt gaatgacatg agcatgatgg agcaacctgt cgagcatagc
     5101 tgctgttaat actttatcgc cggcgaaagt attgtcccat tgaccaaaag cgagattgga
     5161 ggtggcaata atagaacctt tttcatagcg atttgctata acttggaaga aatgatttgc
     5221 ctgatctcgg ttcattggta aatatccaat ttcatcaata atgagcagtc gagcattatt
     5281 aataacccgt cgcataatat ctttatatcg cccttggcga tgagctgttt caagcaataa
     5341 catcaaatct gctgcgctaa taaattttgt tttaatgttt gcttgaactg ctaaataacc
     5401 cagagcaatc gctaaatgag tcttgcctaa tccactagga ccaagtaata aaatattttc
     5461 ctgtcgttca atgaaagcca aagaagtcag ttctcggatt ttattttt
//
LOCUS       DASLOE010000179         5462 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.180, whole genome shotgun sequence.
ACCESSION   DASLOE010000179 DASLOE010000000
VERSION     DASLOE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.180"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..777
                     /locus_tag="U6309_002560"
     CDS             <1..777
                     /locus_tag="U6309_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505986.1"
                     /translation="ASNYFFISGFVISKIKYLPIAVVSSALNLISLLFYLVGYSLWFI
                     ASHFYPSQDKKMQEWYEFAQFKEQYLYAAALGLVATSISMMAIFSPIMLVLSGWLFFG
                     SNIVWTIGEYNKLNNPPSDEENFSKDRQNAYVSYALSISLIGFITAVSTTVAFFIPVM
                     TIPLLIVTTIVCIGVGALAFEYWIESKFGDPQPDITIDESYKQMSNDLGKKITLEPNP
                     TPEPYHGTRPLHFTPHDVKMKEPSPDLQMDLSSRTCKPQY"
     gene            816..1211
                     /gene="cdd"
                     /locus_tag="U6309_002561"
     CDS             816..1211
                     /gene="cdd"
                     /locus_tag="U6309_002561"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEN5505987.1"
                     /translation="MDKLTSNMISEAHKVLVNAYAPYSKFSVAACICTDKNNFYTGVN
                     VENISYGLTTCAEVSAICNMVTAGERKIKSIVVLAANNLLCSPCGACRQRIHEFSSSD
                     TMVHLCNKETIFHSITINELLPLAFDFNP"
     gene            1224..2063
                     /locus_tag="U6309_002562"
     CDS             1224..2063
                     /locus_tag="U6309_002562"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN5505988.1"
                     /translation="MTTSISTISYPHLAAEYIQKTCPSFKPKVGVVLGSGLGQFAEEL
                     EDTVAIEYEKLPGFPRTTVQGHGGKLILGYYGSTAVICLQGRAHTYESMENHETVKTY
                     VRTLKLLGCQYFIATNASGSLKEEVGPGELMLITDHINFQPGNPLVGPNDDEFGPRFY
                     PLDNAYDITMRNALLDIAQRHSIKLHQGVYISVLGPNYETAAEIRAFKLLGADAVGMS
                     TVPEVLVANHCGLRVAVIATITNYATGLAKTSHSHESVVKMASSAAEKLNTLIKQYIL
                     ELS"
     gene            2060..2824
                     /gene="deoC"
                     /locus_tag="U6309_002563"
     CDS             2060..2824
                     /gene="deoC"
                     /locus_tag="U6309_002563"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEN5505989.1"
                     /translation="MSLDKHFNDVLDKLLSNQDIISISNAQLMHCIDLTLLEADASLE
                     ALHQLQSRACQNDVAAICVLPKHLHVFKPNSKIKLATVINFPLGNDDLLVSLAEIDNA
                     IQLGVNEIDYVLPYQYYLRGNKQKALNHCDVIIQTCKKYQLTLKIILETGLFPDMESI
                     YQLSSEVIALGGRFLKTSTGKTPQGATLAAVFAIVSAVMDSTYASSCGIKVSGGIKTP
                     LQAQQYAQLAELLMGKAIHKDWFRIGASTLLEKLLS"
     gene            2878..4650
                     /locus_tag="U6309_002564"
     CDS             2878..4650
                     /locus_tag="U6309_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN5505990.1"
                     /translation="MKLWIMLFNCFIVTFLITFSHAKAPVNNNKSSYCLPNQPCWPTP
                     KEWEQLNKKINGHLIKARNYLSPCIEDPQSNGCKNTLEEIKNPFYLETQPTATQSTGW
                     FDAWDSEISPYIVAAKTAQEIAHAVKFARKHHIKLVVKGTGHDYLGRSNAPDSLLIWT
                     HHMREIQFQKHFVPNGCSANHKGVPAVTAEAGTRWIEAYKEVTVNQGRYVQGGGCTSV
                     GVAGGFIQGGGFGSFSKKYGTGAGGILEAEVIIADGSILIANECQNQDLYWALKGGGG
                     GTYGIVSKITLETHELPATFGALTGSITAKTEQDFKNLIDYFIIFYRERLHNEHWGEQ
                     VILGPDNKMELALVFQGMSKTQVDRLWQPLKTWLSQKPEHYQFTINSLVLPARKFWDY
                     DYLSKNLPDHIVIDKRKNIPYPEFWWAGNQSEVSIYLTHYQSGYLPYTLFQEKFSNKL
                     SNALFEASRLTKLSLHFNKGLAGASQQAIERQRKTSMNPSVLDAAALITIIGGQQYVF
                     PKLPGHKPDLDKARFSAKQAKKAMKLISDLNPDSGTYGNEADYNLKNWQRALWGSNYE
                     KLLQIKHHYDPENLFNCHHCIGSK"
     gene            4730..5128
                     /locus_tag="U6309_002565"
     CDS             4730..5128
                     /locus_tag="U6309_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505991.1"
                     /translation="MKTKIIFKSISSLLLGLLLVSCSNELPPGEYDANEIGKIKKVIP
                     GVIISKRPVNLHRQNADANLHVKGGADSNLIDGGYNRTHGVEYVIKLNSGTIISVVQA
                     EDLKLKNKQKILVIYGRHTRVVADNGSDVY"
     gene            complement(5262..>5462)
                     /locus_tag="U6309_002566"
     CDS             complement(5262..>5462)
                     /locus_tag="U6309_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEN5505992.1"
                     /translation="HSLWLVWAINKNAEWFFYLLWCTAAGILTYQLKLIYARIPKNCF
                     KAFLVSGYYGLVMWFAVGLALI"
BASE COUNT         1766 a         1005 c         1043 g         1648 t
ORIGIN      
        1 gcaagtaatt atttctttat atctggattt gtaatttcaa aaattaaata ccttcccatt
       61 gccgttgttt cttctgccct gaatctgatt tcacttttgt tttacctggt tggctatagt
      121 ttgtggttta ttgcaagtca tttttatcca agccaagata aaaagatgca agagtggtat
      181 gaatttgctc aatttaaaga acaatatctt tacgccgcag ctctagggct cgtcgcaaca
      241 tctatcagta tgatggcaat cttttcccca ataatgcttg ttctttcagg atggctgttc
      301 tttggcagta atattgtttg gacaattggt gagtacaaca aattaaataa tccacccagc
      361 gatgaagaaa atttttccaa agaccgtcaa aatgcctatg tgtcttatgc tttatccata
      421 agtttaatag gattcattac agcagtctca actactgtcg ctttctttat tccagtaatg
      481 actataccct tacttatagt tacaactatt gtatgtattg gggtaggagc gttagcgttt
      541 gaatattgga tcgaatcgaa atttggcgac cctcagcctg atatcactat cgatgaaagc
      601 tacaaacaaa tgagcaatga tttggggaaa aaaattactt tggagcccaa tcctacccca
      661 gaaccttatc atggaaccag gccacttcac tttacgccac atgatgtaaa aatgaaagaa
      721 ccgtctccag atcttcaaat ggatttatcc tctcgtactt gtaaaccaca gtattaattt
      781 agtatattct gcacgccaat gatgagggtg tgacaatgga taaattaact tcgaacatga
      841 ttagcgaagc gcataaggta ctagtcaacg cttatgcccc ctattccaaa ttttcagtag
      901 ctgcctgtat atgcactgat aaaaataatt tttatacagg ggtgaatgta gaaaatattt
      961 cttatgggct aacaacctgt gctgaagttt cagcaatttg taatatggtt accgcaggtg
     1021 agcgaaaaat taaaagcatt gtggtattag ctgcaaacaa tttgctatgc tctccttgtg
     1081 gcgcatgccg acaaagaatt catgagtttt catcctctga taccatggta catctctgta
     1141 ataaagaaac aatatttcat agtattacaa ttaatgaatt actacccttg gcttttgatt
     1201 ttaaccctta atcggacgat tttatgacga cttcaatatc cactatcagt tacccacatt
     1261 tagcagcaga atatattcaa aaaacatgtc catcatttaa accaaaagtg ggtgtagtat
     1321 taggctcagg gttaggtcaa tttgccgaag agttagaaga tacagttgct attgaatacg
     1381 aaaaactccc aggatttccc agaactacag tacaggggca tggaggaaaa ttaattttag
     1441 gatattatgg atctactgcg gttatttgct tacaaggcag agcccatact tacgaaagca
     1501 tggaaaatca tgaaacagta aaaacctatg taagaacttt aaagctatta ggttgccagt
     1561 attttatagc aacaaatgct tctggttcac ttaaagaaga agttggaccg ggtgaattaa
     1621 tgctaattac cgatcacatc aattttcaac ccggaaatcc cctggttggt ccaaatgatg
     1681 atgagtttgg acccaggttt tatcctttag ataatgctta tgacattacc atgcgtaatg
     1741 cattattgga tattgctcaa agacactcga ttaaactaca ccagggggtt tatatctctg
     1801 tcttgggacc aaactatgaa acagctgctg aaattcgtgc ctttaaattg ctgggagctg
     1861 atgcagtggg gatgtcaact gtgcctgaag ttttagtcgc caatcattgt ggcttgcgtg
     1921 tcgctgtcat tgcaaccata acaaattatg caactggcct tgcaaaaacc agtcatagcc
     1981 atgaatccgt tgtcaaaatg gcttccagtg ccgctgaaaa acttaatact ttgattaaac
     2041 agtatatttt ggaattatcg tgagtttgga taagcatttt aatgatgtct tggacaagct
     2101 cctgtcaaac caggatatta tatctatttc gaatgctcaa ttgatgcatt gcattgattt
     2161 gactctgcta gaggcggatg cttctcttga agcattgcat caattacaga gccgagcctg
     2221 tcaaaatgat gttgcagcta tttgcgtact acccaaacat cttcatgttt ttaagcctaa
     2281 ctccaaaatt aaattagcaa ctgttatcaa ttttcctctt gggaatgatg atttgctggt
     2341 ttccttggca gagatagata atgccattca actgggtgtt aatgaaatag attatgtact
     2401 accctatcag tattacttaa gaggaaataa gcaaaaagcg ctaaatcatt gtgatgtcat
     2461 tattcaaacc tgcaaaaaat atcaacttac cttaaaaata attttagaaa ctggcctttt
     2521 tccagatatg gaaagcattt atcagcttag ctcagaagtc attgctcttg gtgggcggtt
     2581 tctcaaaact tcgactggaa aaacccctca gggtgcgact ctggcagcag tatttgctat
     2641 agtcagtgct gttatggatt caacttatgc atcctcttgt ggaattaagg tttcaggagg
     2701 gattaaaaca cctctccagg ctcaacaata tgctcaactg gctgaattac ttatgggcaa
     2761 agcaattcat aaggattggt ttcgtattgg agccagtact ctgcttgaga aattattgtc
     2821 atgaacgcac aatctatgtg ttaatatgtc agcctgcaaa cagggagagg tcttgcaatg
     2881 aaattatgga taatgctatt taactgcttt attgttacct ttttaataac attctcccac
     2941 gcaaaagcgc cagtaaataa caataaatca tcttattgcc tccctaacca accatgctgg
     3001 ccaaccccca aagaatggga gcaactaaac aaaaaaataa atggccattt aataaaagcc
     3061 aggaactatc tgtcaccttg cattgaagat ccccaaagta atggttgtaa aaataccctg
     3121 gaggaaataa aaaatccctt ttatttggaa acacaaccta ccgcaaccca gtccaccggc
     3181 tggtttgatg catgggattc agaaataagc ccatacatag ttgcagcaaa aacagcgcag
     3241 gaaattgctc atgcagtaaa atttgctaga aaacatcata tcaaactagt tgtcaaaggc
     3301 actggtcatg actatcttgg aagatccaat gctcctgatt ctttgctgat ttggacacat
     3361 cacatgcgag aaatacaatt ccagaaacat ttcgtcccaa atggttgttc tgcaaatcat
     3421 aagggtgttc ctgcggtaac tgctgaagca ggtaccagat ggatagaggc ttataaagag
     3481 gttactgtaa accagggtcg ctatgttcag ggtggaggct gtacttccgt tggagttgct
     3541 gggggattta ttcaaggcgg gggatttggg agtttttcta aaaaatatgg aaccggtgca
     3601 gggggaattt tagaagcaga agtaattatc gctgatggtt caattttgat tgccaatgaa
     3661 tgccaaaacc aggatttata ttgggcattg aaaggaggtg gcggcggaac ttatggtatt
     3721 gtaagtaaaa tcacattgga aacgcatgaa ttgccagcaa catttggagc attaacagga
     3781 agtattacag caaaaacaga gcaggatttt aagaatctca tagattattt tattattttt
     3841 tacagggaaa gacttcataa tgaacattgg ggggaacaag tcatattagg ccctgataac
     3901 aaaatggagt tggcattggt ttttcaaggg atgagcaaaa ctcaggttga tagattatgg
     3961 caaccattaa aaacttggtt atcccaaaaa cctgaacatt atcaatttac aataaacagc
     4021 ctggtactgc ctgcaagaaa attctgggat tatgattatc taagcaaaaa tctacctgat
     4081 catattgtaa ttgataagcg aaaaaatatc ccttatccag agttttggtg ggctggtaac
     4141 cagtcagaag tttctatcta tttaactcac taccaatccg gttatttgcc ctatacgctc
     4201 tttcaagaaa aattttcgaa taaactatct aacgctttgt ttgaagcatc tcgcctgacc
     4261 aaactgtctt tgcattttaa caaaggcttg gcaggggcgt ctcagcaagc aatcgagaga
     4321 caaagaaaaa catcaatgaa cccttccgta ttggatgcag cagccttgat aacaataatt
     4381 ggggggcaac aatatgtctt tcccaagttg cctgggcaca aacctgattt agataaagca
     4441 cggttttcag ctaagcaagc aaaaaaagca atgaagctaa tatctgatct gaatcctgat
     4501 tcaggcactt atggaaatga ggccgattac aatttaaaaa attggcaaag agctttatgg
     4561 ggatccaatt atgagaagtt attacaaatt aaacaccatt atgatcctga aaacctgttt
     4621 aattgccacc attgtattgg aagtaaataa aaattaatta ataattttca gaatctcagt
     4681 tagaataact agtcatagtg aattaattag cattggagtt taaattatca tgaagactaa
     4741 aatcatattt aaatccattt catcattact attgggtttg ctacttgtaa gttgtagcaa
     4801 tgaacttcct cccggcgaat atgatgcaaa tgagataggc aaaataaaaa aggttattcc
     4861 gggtgtgatt atttccaaac gaccagttaa tttacatcgt caaaacgctg atgccaatct
     4921 gcatgtaaaa ggtggtgctg attctaattt gatagatggt ggttataata gaacacatgg
     4981 tgtcgaatac gtcatcaagt taaactctgg caccattatt tcagtggtac aagcagaaga
     5041 cttgaaatta aagaacaagc aaaaaattct ggtaatttat ggaagacaca ctcgagttgt
     5101 agctgataat ggaagcgatg tatattaatt agcctgcctt ctttaaaata tgggttggtg
     5161 ttggattttt tcttctaaag tctgtcttat gaaggaattt tccgacaccc acaatctctt
     5221 ggcacaacaa attttctatt agggttgaga ggacatatta tttaaatcaa tgctaatcca
     5281 acggcaaacc acatcactaa tccataataa ccactcacta aaaatgcttt aaaacaattt
     5341 ttaggtattc ttgcataaat taattttagt tgataagtca atattccagc agcggtacac
     5401 cataacaaat aaaaaaacca ttctgcgttc ttatttattg cccataccaa ccataaagaa
     5461 tg
//
LOCUS       DASLOE010000180         5425 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.181, whole genome shotgun sequence.
ACCESSION   DASLOE010000180 DASLOE010000000
VERSION     DASLOE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.181"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            211..882
                     /locus_tag="U6309_002567"
     CDS             211..882
                     /locus_tag="U6309_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5505993.1"
                     /translation="MESLMPKAFTHLCKKINLSLYNDTLTVYPKSSALPKPIQIRSEL
                     DLLMVLLFSQMDCNSAQVDKFFMMRSNELGVYRFIHRDDTDHDLYHQDEFSMDDNHYH
                     AQFDKTIDEIKLENILGILEKHSLISSEEHVSFIEAYHKANTLTEDTPKLETTLSSPL
                     PISSPSVHSGAKKPVVKEKEVDTPTTFAGLRVGFFLTSTKTTTKPLSRLHDDEKKIGR
                     NGHFH"
     gene            1139..2515
                     /gene="ffh"
                     /locus_tag="U6309_002568"
     CDS             1139..2515
                     /gene="ffh"
                     /locus_tag="U6309_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEN5505994.1"
                     /translation="MFENLTERLTRTFKNLRGQGRLTEDNIQHALREVRLSLIEADVA
                     LPVIKEFIEQVKQKSLGQEVLTSLNPDQAFIKIVHDELIHVMGDERVELDYKTQPPAV
                     FLMAGLQGSGKTTSTAKLARYLKETENKKVMMASVDVYRPAAIHQLKVLAEQIDVAFF
                     DSDPNEQPIKIAQKALESARKQYVDILILDTAGRLHVDEDMMSEIKSLHKAVNPIETL
                     FVVDSMTGQDAANTAKAFHEALPLTGVILTKTDGDARGGAALSVKQITGKPIKFIGSG
                     EKIDALEPFHPERIASRILGMGDILTLIEEVERKADKQASEKLAKKLKKGKGFDLEDF
                     KQQLLQMNNMGGISGMMSKLPGVGQLPKQAMQQVNDKAMAQTIAIINSMTPKERRIPK
                     IIVGSRKKRIALGSGTQIQDVNRLLKQFEQMQKMMKKFTKPGGMQKMMRGIGGMAGLK
                     GMFPDDLK"
     gene            2602..2862
                     /gene="rpsP"
                     /locus_tag="U6309_002569"
     CDS             2602..2862
                     /gene="rpsP"
                     /locus_tag="U6309_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEN5505995.1"
                     /translation="MVVIRLSRAGAKKRPFYHMVVTDSRKRRDGNYIERIGYFNPVAR
                     GQEVKLHIDMDKMTHWQKVGAQLSDRVSALLKEHSKKSETAA"
     gene            2868..3377
                     /gene="rimM"
                     /locus_tag="U6309_002570"
     CDS             2868..3377
                     /gene="rimM"
                     /locus_tag="U6309_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEN5505996.1"
                     /translation="MNNKTNWVIIGRFGRPHGIKGFVTVHSFTDPADNILRYNDWHVF
                     LNKQWQPLKLLTIEVRSKAIIAQIEGYPERELVSALTNLDIGVQESQLAALAPGEYYW
                     YQLIGMSVINSKGDLFGKVVEIMPTGSNDVLVVEGEKRHLIPYLPGQFVINIDESQQV
                     ITVDWDMNF"
     gene            3377..4129
                     /gene="trmD"
                     /locus_tag="U6309_002571"
     CDS             3377..4129
                     /gene="trmD"
                     /locus_tag="U6309_002571"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEN5505997.1"
                     /translation="MAIHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSS
                     RPYKQVDDKPYGGGPGMVMMYEPLHAAIKHARSEMKENCKTIYLSPQGKAVRQNDLKQ
                     IATQKQSLLFVAGRYEGIDERIINHHIDEEWSLGDFVLSGGELAAMVFIDAIIRLIPG
                     SLGHLGSAEQDSFMNGLLDCPHYTRPATINGLDVPDVLLGGNHKEIERWRRKQSLGKT
                     WLKRPDLLEKIQLSETDKQLLAEFKCEHGDSC"
     gene            4156..4521
                     /gene="rplS"
                     /locus_tag="U6309_002572"
     CDS             4156..4521
                     /gene="rplS"
                     /locus_tag="U6309_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEN5505998.1"
                     /translation="MTNIIDQINAEQMQGKEIPDFNPGDTVLVQVKVIEGNRERLQAF
                     EGVVIAKRNRGLNSAFTVRKISHNVGVERVFQTYSPIVDSITVKRRGDVRRAKLYYLR
                     NLAGRAARIKEKLSGKKGD"
     gene            4537..4992
                     /locus_tag="U6309_002573"
     CDS             4537..4992
                     /locus_tag="U6309_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEN5505999.1"
                     /translation="MLLVSIFNTPVGNLEVAHDEHFIFNASFTDKSGAPAQNQLAHLI
                     ASELKAYFDDPHHRFQLPLKPQGSLFQQKVWNTLLVIPVGRTVTYGELATKLQSSPRA
                     VGQACKCNPLALFVPCHRVVGKHDLGGYMGNPNAISYKKNLLAHEAKII"
     gene            complement(5093..>5425)
                     /locus_tag="U6309_002574"
     CDS             complement(5093..>5425)
                     /locus_tag="U6309_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg0393 family guanine-nucleotide exchange
                     effector"
                     /protein_id="HEN5506000.1"
                     /translation="YLSLLESAHLPNDQLKSSRISLVVRLAKYLNEQTELNQDVHDEI
                     AVYVKKLWEMKPAEFEEEFLKKISPLPFIDNTVRILTGLSARFFSILQPPSPPLNINS
                     DVDKNPKV"
BASE COUNT         1784 a         1035 c         1113 g         1493 t
ORIGIN      
        1 agaaaatctc agtaaaaaga gagaaatgga aatagggcca gtcaatacaa atcaaaagct
       61 cggttgtaca gcaaatccga tttcctggaa gcaacagatc agttattgca attctttgat
      121 agcacctctt gtttcttctt aagaaaacct taatgttgat gttttaaact atggggccaa
      181 aatatagaca actgcttttt tttaaaatca ttggagtccc ttatgcctaa agcatttacc
      241 catttgtgta aaaaaatcaa tctcagttta tataacgata cattgactgt ctaccctaaa
      301 tcatccgcac tgccaaaacc tattcaaata cgtagtgagc ttgatcttct tatggttctg
      361 ttgttttctc aaatggattg caatagtgca caggttgata aattttttat gatgcgttcg
      421 aatgaacttg gtgtctatcg ctttatacat cgtgacgaca ctgatcatga tttgtatcat
      481 caagatgaat ttagcatgga cgataaccat tatcatgctc aatttgacaa gactattgat
      541 gagattaaat tggaaaatat tctgggaata ctagaaaagc attctcttat ttcgtctgaa
      601 gaacacgtaa gctttatcga agcctaccat aaagccaata cgctcaccga agatacgcca
      661 aaactggaaa caactctgtc tagtcctttg cctatatcct ctccttcagt tcactcaggc
      721 gcaaaaaaac ctgttgtgaa agaaaaagaa gtagatactc caacaacttt cgcgggacta
      781 agagtaggat ttttccttac ttcaacaaaa acaacaacaa aacccttatc cagacttcat
      841 gatgatgaga agaaaatcgg aaggaatggg catttccatt aattgatata gtttgataaa
      901 ccatagtaaa gatcgttcta ttggtttact taatatgaca atttacggtg atttacccaa
      961 aatctaaaaa actgtagagt aactaataaa ttactctaca gcttctaatt acaaaatcat
     1021 aaaaataacc ctaattgatt cactgttatc aattactgtg actcattaat ccgtttttct
     1081 ctgtggtata cttttaactc cctatgttat agtagttatt ttttaggtac tacttattat
     1141 gtttgaaaat ttaaccgaac gcttaacccg gacctttaaa aatttgaggg gtcaaggtcg
     1201 cctgacagaa gacaatatcc agcatgcttt gcgtgaagta agactgtctc ttattgaagc
     1261 agacgtggct ttgcctgtta tcaaagagtt tattgagcaa gtaaagcaaa agtctcttgg
     1321 gcaggaagtc ttaaccagtt taaatcccga tcaggcattc attaaaatcg tgcatgatga
     1381 acttattcac gtcatgggcg atgagcgggt tgaacttgat tacaaaaccc aaccgcctgc
     1441 tgtcttttta atggccggct tacaaggttc tggtaaaaca acgagtacag caaaactcgc
     1501 gcgttatttg aaagaaaccg aaaataaaaa agtcatgatg gcaagcgttg acgtatacag
     1561 accagccgcc atacaccaac tgaaagtgtt agccgaacaa attgacgtcg ccttctttga
     1621 ttcagaccct aatgagcaac ccataaaaat agctcaaaaa gcattggaaa gcgccagaaa
     1681 acagtatgtt gacattctca ttctcgatac agcagggcgc ctgcacgttg atgaagacat
     1741 gatgtctgaa atcaagtctt tgcataaagc agtcaatcca atcgaaaccc tttttgtcgt
     1801 tgacagcatg acaggacaag acgctgccaa tactgcaaaa gcatttcatg aagccctccc
     1861 tctgactggt gtgattttga ccaaaacgga cggcgatgct cgaggaggag cggcgctatc
     1921 tgtcaaacaa atcacaggga aacctataaa atttataggg agtggtgaaa aaatagacgc
     1981 cttggaaccc tttcaccctg aacggattgc atcaagaata ttggggatgg gagatatttt
     2041 aactctcatt gaagaggtag agcgcaaagc cgacaaacaa gccagcgaaa aactggccaa
     2101 aaaattaaaa aaagggaaag ggtttgactt agaggatttc aaacaacaat tattgcaaat
     2161 gaacaatatg gggggtatct ccgggatgat gagcaaatta cccggagtgg ggcaattgcc
     2221 aaagcaagcc atgcagcaag taaatgacaa agcgatggct caaaccattg caatcattaa
     2281 ctctatgact cccaaagaac gccgtatacc caaaattatt gtaggctcac gtaaaaaacg
     2341 catagcctta ggctctggaa ctcaaattca ggatgtcaac agactattaa aacaatttga
     2401 gcagatgcaa aagatgatga aaaaatttac caaaccaggc ggaatgcaga aaatgatgag
     2461 aggaattggt ggcatggccg gattgaaagg catgtttcca gatgatttaa aataattcgt
     2521 aagaattact tcacatgtcg atgtaatttt cgtacaatac acggcatttt tatgtaacca
     2581 ctctaacagt agaggaaaat aatggtcgtt atacgtttat cacgagctgg cgctaaaaag
     2641 cgtccttttt atcacatggt tgttactgac agccgcaaac gtcgcgatgg aaactacatt
     2701 gagcgtattg gttattttaa ccctgtagca cgtggacaag aagtaaagct tcacattgat
     2761 atggataaaa tgacccactg gcaaaaagtt ggggcccagt tatccgatcg agtcagtgcc
     2821 ttgttaaaag aacatagcaa aaaatcagag acagctgctt aaccaaagtg aataacaaga
     2881 caaattgggt tatcatcggc cgttttggcc gaccccatgg tattaaaggc tttgtcacgg
     2941 tacattcctt cacagaccct gctgataata tcttgcggta caatgactgg cacgtttttt
     3001 taaataaaca gtggcagccc cttaagttat tgacaataga agtaagaagt aaagcgatta
     3061 ttgctcaaat tgaagggtac ccagagcgtg aattggtctc tgcattaacc aatttagata
     3121 ttggtgtgca agaatcacag cttgcggctt tggcaccagg tgagtattac tggtatcaac
     3181 tgattggcat gagtgtcata aattccaaag gcgatctttt tggaaaagtt gttgaaatca
     3241 tgcctactgg ctcgaatgat gtacttgttg tggaaggtga aaaaagacat ttaattccct
     3301 atcttccagg acaatttgta atcaatattg atgaaagcca gcaagtaatc actgttgatt
     3361 gggatatgaa tttttagtgg caatacatct tggagtcatc accctgttgc ctgaaataat
     3421 acaaggcatc cattatgggg taaccggccg agctattgaa caaggcttgg ttaaaattga
     3481 ttgctggaat cctagggatt ggtcatcaag accttataag caagtggatg acaagcccta
     3541 tggtggtgga ccaggaatgg tcatgatgta tgaaccattg catgctgcaa taaagcatgc
     3601 gcgcagtgaa atgaaggaaa actgcaaaac aatttactta agcccccaag gcaaggcagt
     3661 tagacagaac gatttaaaac aaatagctac ccaaaaacaa tccttgctat ttgttgcagg
     3721 gagatatgaa ggaattgatg agcgaatcat taatcaccac atagatgaag aatggtcgct
     3781 aggggatttt gtattaagtg gcggtgaatt ggccgcaatg gtatttattg atgcgataat
     3841 tcgcttgata cctggaagcc ttgggcattt gggttcagca gaacaagatt catttatgaa
     3901 tggattactg gactgcccac attatactcg gccagcaact ataaatggac tggatgtacc
     3961 tgatgtttta ttaggaggaa accataaaga aatagaacgc tggcgtagga aacaatccct
     4021 gggtaaaacc tggctcaaac gcccggattt acttgagaaa atacaattaa gtgaaacaga
     4081 caagcaattg cttgctgagt ttaaatgcga acacggcgat tcctgctgag gataagccaa
     4141 tttgaggagt agtctatgac taatattatt gaccaaatta atgccgaaca aatgcaaggc
     4201 aaagaaattc ctgatttcaa cccaggcgat actgttctgg tccaagtaaa agttattgaa
     4261 ggtaaccgtg agcgtttaca ggcatttgaa ggcgttgtga ttgccaagcg taaccgtgga
     4321 ctaaactctg ctttcactgt acgtaaaatt tcccataacg ttggtgtaga gcgcgttttc
     4381 caaacctaca gtcctattgt tgatagtatt acggtgaaaa gacgtggtga cgttcgtcgt
     4441 gccaaattat attacttaag aaatcttgct ggccgcgcag ctcgtattaa agaaaaatta
     4501 tctgggaaga aaggcgatta attcttgaat ggtaaaatgc tcctcgtgtc tatttttaat
     4561 actcctgtgg gtaacctgga agtagcccat gatgagcatt ttattttcaa tgcgtccttc
     4621 acagacaaat cgggcgctcc agcccaaaac caattggccc atttaattgc cagtgaattg
     4681 aaggcttact tcgacgatcc ccatcaccgt tttcagctcc ctctgaaacc tcagggaagc
     4741 ctattccagc aaaaggtttg gaatacgttg ttagtgattc ccgttggcag gacagttacc
     4801 tatggagaac tcgctacaaa actgcaaagt agtccaagag cagttggaca agcctgtaaa
     4861 tgcaacccac tggctttatt tgttccttgt catagagtag tagggaagca tgatcttgga
     4921 gggtatatgg gaaatccaaa cgccatttct tataaaaaaa atctgctcgc tcatgaagca
     4981 aaaatcatct gatgaattaa atttataata atcgatatta cattgctcag aacttataaa
     5041 aacgccaagg caaaaaatat ttctaacgaa gaaattagca aatcgtgaga gattaaactt
     5101 tgggattctt atccacatca gaattgatat tcaaaggtgg ggaaggaggt tgtaatatac
     5161 taaaaaatct ggcacttaat cccgttaata ttcgtacagt attatcgata aacggcagcg
     5221 gcgaaatttt ctttaaaaac tcctcttcga actcagcagg cttcatttcc caaagctttt
     5281 ttacatacac ggctatttca tcatgaacat cttgatttaa ttcggtttgc tcattgagat
     5341 acttggctaa tcgaacaacc aagctgatgc gagaagattt taattgatca tttggcaagt
     5401 gtgcagactc taataaacta agata
//
LOCUS       DASLOE010000181         5366 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.182, whole genome shotgun sequence.
ACCESSION   DASLOE010000181 DASLOE010000000
VERSION     DASLOE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.182"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..586)
                     /locus_tag="U6309_002575"
     CDS             complement(<1..586)
                     /locus_tag="U6309_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEN5506001.1"
                     /translation="MIPEKLLTYTKTIFEYSHSTVYIKDKNFHYQYMNNKGLKQLQLQ
                     EKDIIGMPDKALPFGKYAHFYNSHDYSAMEEIIYFQLDDCITKNGEKIFALSHKLPLK
                     DEDGKTHGVIGFTKFMNLHQLMSNLNSKNTFLTPNLKINVNVDDWLKTTINLSRREME
                     VLYYFLQGKSIKLIAPLLHITERTVIFHLNNIKSK"
     gene            complement(979..1872)
                     /locus_tag="U6309_002576"
                     /pseudo
     CDS             complement(979..1872)
                     /locus_tag="U6309_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1954..3198)
                     /gene="ltrA"
                     /locus_tag="U6309_002577"
     CDS             complement(1954..3198)
                     /gene="ltrA"
                     /locus_tag="U6309_002577"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN5506002.1"
                     /translation="MEKTKPFNIPKQLFVQAYKLVKANAGAAGVDEESLEDFAKDLKN
                     NLYKLWNRMSSGSYFPPAVKAVPIPKKSGGERMLGIPTVADRIAQMVVKLVFEPIVEP
                     HFHPDSYGYRPNKSALDAVGITRQRCWQYDWVLEYDIRGLFDNIDHQLLMKAVRKHTD
                     SKWVLLYIERWLVTPMQLPDGTLQEKVKGVMQGGVISPVLSNLFLHYVFDSWMVRNAT
                     KMSWCRYADDGLVHCKTKFEAQQIRRRLEARFIECGLEMHPDKTKIVYCKDSNRRLNY
                     QNTSFDFLGFTFRPREAKNQRNGIRFTSFTPAASKAAMKSMRAKIKKCRLGRRTELSI
                     HDIVDRCNPYLRGWVNYYGQYHITEMESVFRHFNQSLVKWAMRKYIRLNKTQAIEFLS
                     NLAKEKPRLFVHWDRGRPGAFA"
     gene            complement(3762..4022)
                     /locus_tag="U6309_002578"
                     /pseudo
     CDS             complement(3762..4022)
                     /locus_tag="U6309_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611582.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4556..5365
                     /locus_tag="U6309_002579"
     CDS             4556..5365
                     /locus_tag="U6309_002579"
                     /inference="COORDINATES: protein motif:HMM:NF037126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506003.1"
                     /translation="MNFSQREGYKDISLSPFEGMSTGLKNAIFNEIHSVINEFISLGM
                     TGYGDSSYKGKNAELLWTVFFEKKKSILPYAGDFLKEFESLYDSLKWYEIYDLIEYLI
                     TLSNLRNDLIEVKFNQLFEKYNSPYRLINKVVQPISSEETIAMINTAFENAPTQESKQ
                     HLENAEKHYSRKQDPDFNNSCLESIKAIESCLRSKFKNKDNLGDNIKKLRGASYNQHI
                     VSIIDKINAFRGHDSAHATKPDGYKPTREDAILIHSICCSFINYFTFNKKG"
BASE COUNT         1600 a         1096 c          877 g         1793 t
ORIGIN      
        1 atttgctttt tatattatta agatgaaaaa taacggttct ttcagtaata tgaagcaatg
       61 gtgcaataag cttgatcgat tttccttgta aaaaataata caaaacctcc atttctctcc
      121 gagacaaatt aatcgttgtt tttagccaat catcaacatt aacattgatt ttaagattag
      181 gtgttaaaaa ggtatttttt gaattaaggt tactcatcag ttgatggaga ttcataaatt
      241 tcgtaaagcc aatgacgcca tgagtttttc catcctcatc tttaagcggt aatttgtgag
      301 acaaggcaaa tattttttca ccgtttttag taatacaatc atccaattga aagtaaataa
      361 tttcttccat tgccgagtaa tcatgactat tataaaaatg cgcatatttc ccaaagggaa
      421 gtgctttatc aggcatgcct ataatatctt tttcttgtaa ttgcaattgt ttgagtccct
      481 tattattcat atactggtaa tgaaaattct tatctttgat gtatacggta ctatgggagt
      541 attcaaaaat tgtttttgta taagtcaaga gtttttcagg gatcataaag ctattgtgac
      601 acaatcttct tttgattaaa aatcatacca caagatcata aataaatcaa cttgcttttt
      661 tttattagat catgaactac ttattttaat taataaatct tggattgatt gtattgttgg
      721 gtcaaaatga cccaacctcg ctttgtaaac gatgtgattt ctctggatga atttagttgt
      781 aaattgacta ttattgttaa cctcaataga attgccatcc aatttatcat ctcggtctat
      841 taatgggcat attttatatt ttttcaaacc aactaactga tatcgataat cctcaaattc
      901 aaaacattaa aaaaggtagg gtatttgatt ctgagcataa gatgaattga cgtggtcaac
      961 caaaatgtta cacctcagtt aagcaacttt cattaaaaaa tcgctctgtt tttcaaattg
     1021 atttggactt aaataaccca aatatgaatg taatctgtga ctgttataga acatggtgat
     1081 gtaattcaaa atgtcctgtt gagcctccca tcgagtttga taatttcgcc actgaactcg
     1141 ctcttgtttt aagctgccaa agaagctttc aacaacgctg ttatcccagc aatcaccttt
     1201 tctgctcatg ctaccaatat atcctttgcc cgttaataaa ttgcggtatt ggcaactagc
     1261 atactgagcc cctctatcgg aatgaacaat aagccctgat ggaggttttc gctgccaaat
     1321 tgccatattc agagcatcac aaaccaatgt tgctttcatt ctggatccca tgctccaacc
     1381 aaccaccttc cttgaaaata aatccagaac gactgctaaa taaagccagc cttcctgcgt
     1441 ccagatatag gtaatatctg atacgtaggc tttattaggt ttatctgtat gaaattgcct
     1501 attcaataca ttttcataaa ccggttgttt atggttgcta ttggtagtca ccttatattt
     1561 ctttcgatat cgaacaaaaa ctttggcttc tttcattaaa ctttgagctc gtcttcgacc
     1621 tactggataa cctaatgcat ttaaagcatt cttcatcctg cgacttccat aacaatactg
     1681 gctcgcctca gctattttct tcacacaatc aataacctcc tcatgttcag gatcatctgg
     1741 tttatttctc tggcgttgct gataactgta atatccatgc cgtgtaatgc ccaggagttg
     1801 gcacattaag ccaaccggcc aggtcttctt attttgggcg acaaacgaat attttatttc
     1861 gtttcttgag caaagaggtg agtagaccgg tggaacttcc ccaccagccc ctctcaaaac
     1921 ggagcgtgaa cctctcgact cactccgctt ccactaagca aacgcacctg gccttcctct
     1981 atcccagtgt acaaataacc tcggcttttc cttggcgaga ttactcagaa actcaattgc
     2041 ttgtgtttta ttgagcctta tgtattttct cattgcccat ttgactaatg attgattgaa
     2101 atgtcgaaaa accgattcca tttcagtaat gtgatattgt ccataataat taacccaacc
     2161 gcgaaggtat ggattacatc gatcaacaat atcatgtatc gatagctccg ttcgtctacc
     2221 taatcggcat ttcttgatct ttgcccgcat tgatttcatc gcggctttgc tcgcagctgg
     2281 agtaaagcta gtgaatcgta taccattcct ttgattcttc gcctccctcg gtctgaatgt
     2341 aaatcctaga aaatcaaacg aggtgttttg atagttcaat ctacgattac tatctttgca
     2401 gtaaacaatc tttgttttat caggatgcat ttctaaacca cattctataa aacgcgcctc
     2461 taacctgcgt cgaatttgtt gtgcttcaaa ctttgttttg cagtgtacta aaccatcatc
     2521 tgcataacga caccatgaca tctttgtagc atttcgcacc atccatgaat caaaaacata
     2581 atgtagaaat aggttactta atactggact gataacccca ccctgcatca cacctttgac
     2641 tttctcttga agagtgccat ctggtagttg cataggagtt accagccatc tttctatata
     2701 gagtaatacc catttactgt ccgtatgctt tcttactgct ttcattagaa gttgatgatc
     2761 gatgttgtca aacaggcctc gaatatcgta ctctaacacc caatcatatt gccaacatct
     2821 ctgtcgcgtg atacctacgg catctaacgc tgatttgtta ggcctgtatc catatgaatc
     2881 tggatgaaaa tgaggttcga caattggctc gaaaactaac ttgaccacca tctgcgctat
     2941 tctgtctgcc accgtgggaa tgccaagcat cctttcccca cctgacttct tgggaatagg
     3001 aaccgctttc accgcaggag gaaaatagct tccagatgac attctgttcc aaagtttata
     3061 aagattgttc ttcagatctt ttgcaaaatc ctcaagggat tcttcatcaa caccagccgc
     3121 accagcattt gcttttacaa gtttataggc ttgtacaaat agttgtttcg gaatgttaaa
     3181 tggttttgtc ttttccaact atttcctcct gaatgaatca gttgaatatt gaattagact
     3241 gcttagcaca gcccctttgc tctgacgcca ttacagcgtc ttcttcgcta ctacgagctg
     3301 ttccgtccca gtgctttgca tcggtactct catccttatg aatgcgtcac ttggatttct
     3361 cccttatcat caaaacgact ggttcccaca gttccataaa gaagcccgaa ttagactcac
     3421 gccatcttca caccgagtac cacttgcgca gctttcaggt ctactcgcaa atttgtccca
     3481 aagtacagtc taaaccttgg tttcgatact atgtttaaag ctaacgatgc ttcatcgatg
     3541 gttcattggt attcgtcttt ctaatccata cctgccaagt gttcttgact tttcttcaac
     3601 gctcaccacc tttgctctta acaaaagcag cttgaagtgg tttgatgcct gttcctgaca
     3661 accgacaccg atgggtctac catcatcttc cttacagctt taccaaaata atattcaatc
     3721 cttgaatatt attggtgtgc ctgtggcaca cagaccgccg ccttttttaa gatttccttt
     3781 tccattttca agcgtttatt ttcttcctgc agacgacgta tctctaattg ttctgctgtt
     3841 aacttgccat ttcctcgaaa tgcttgccca tcaggctctt ggtgctcttt aagccagcga
     3901 ctaagtagag cttgatgaat ccccaaactt ttagccgctt ctgtgcagcc atagccttgt
     3961 tctaatacca gactaactgc atcaagtttg aattctttag aatacttctt tcttgctacc
     4021 atttttttct ccagttaaat acattatctc ttaactgggg tgtcaaaatc tattagacca
     4081 cgtcaattgt ggtttgtctt ggccaattaa gacattcaaa aaataaaaaa tctataaaat
     4141 gtaagcgagt agtcgattca tttcgctgtc gagtagaagt tttaaaatca ttgttcaatt
     4201 ttgtaagatt ttgcctacat agctcgtacc tattatccat aatattccta ccctaaagaa
     4261 caatctcata cactttagaa tcttttaatc ctcataactc atcatattat tggtttacct
     4321 tattccttaa gtattgattc gatatgttaa cttttattat agtttattaa taactctaat
     4381 ggattgaata gtagtcaaaa tgaaaatttt tcagcttaat aaagttagtg gccattttgg
     4441 ttgtaaaaat agttatttcc aataattttt gaactatatt tatgcagtag actcacgcga
     4501 aagtttgaga atgatttcgt aaaaggtaag taactggaaa gtgtagggag aactaatgaa
     4561 ctttagccaa agagagggtt ataaagatat aagccttagt ccatttgaag ggatgagcac
     4621 gggtttaaaa aatgcaattt tcaacgaaat tcactctgta ataaatgaat ttatttccct
     4681 agggatgact ggatatgggg attcatcata caaaggaaag aatgccgaac tgctttggac
     4741 tgtttttttc gagaaaaaaa aatctatctt gccttatgct ggagattttt taaaggaatt
     4801 tgaatcttta tatgattcgc tgaaatggta tgaaatctat gaccttatag aatatttgat
     4861 caccctgtca aaccttcgaa atgacctgat tgaagtaaag ttcaaccagc tctttgaaaa
     4921 atataattca ccctacagac tgattaataa agtagtacag ccaatctcta gtgaggaaac
     4981 tattgcaatg attaatacag cctttgaaaa tgcccctact caggaatcta aacagcattt
     5041 ggaaaatgca gaaaagcact attctagaaa gcaagatcca gactttaata attcatgttt
     5101 agagtcaata aaagcaatcg aaagctgctt gcgttctaag tttaaaaaca aggataatct
     5161 tggtgataat ataaaaaagc taagaggagc atcttataat caacatatag tttctattat
     5221 agataaaata aacgcattta gagggcatga tagtgctcat gctactaaac cagatggata
     5281 taagccaact agagaagatg ccattttaat tcacagtatt tgttgtagtt tcattaacta
     5341 ctttactttt aataaaaaag gataaa
//
LOCUS       DASLOE010000182         5341 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.183, whole genome shotgun sequence.
ACCESSION   DASLOE010000182 DASLOE010000000
VERSION     DASLOE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.183"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..340)
                     /locus_tag="U6309_002580"
     CDS             complement(<1..340)
                     /locus_tag="U6309_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506004.1"
                     /translation="MWYDTLLEYLGETICPSNKEKARLFISHLFKLLKENSYFGENFE
                     PEFIFGVMVALYNYSISHENHNVTLDEFGKYCMGLIMVFSKISDDLSIYCSDFIYILS
                     DKSILQELKIE"
     gene            523..612
                     /locus_tag="U6309_002581"
     tRNA            523..612
                     /locus_tag="U6309_002581"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:557..559,aa:Ser,seq:gct)
     gene            619..695
                     /locus_tag="U6309_002582"
     tRNA            619..695
                     /locus_tag="U6309_002582"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:653..655,aa:Arg,seq:acg)
     gene            complement(719..2005)
                     /gene="legL2"
                     /locus_tag="U6309_002583"
     CDS             complement(719..2005)
                     /gene="legL2"
                     /locus_tag="U6309_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegL2"
                     /protein_id="HEN5506005.1"
                     /translation="MRVELFNPSIKKIIVKLNQLKGLWIVQLNLSKNRLSEKRFTDLA
                     VLLSMLKAFGVTYLNLHFNNLGQKTGTELVALFKILKDSGISRLSLSANELYQKTGTE
                     LATLLTVLSDSDIIQLDLSCNWLGQRPDAELIELFKAWSSTPIIDLDLTNNDLGKKSG
                     IVLSEMFRALKVSSISHLGLRNNDLYQRTGAEFSSLLSAIKDSPITRLDLSDNGLHLM
                     VVTAFETLFDALKDTSVTHLNLCRNGIGYLDMFEMTAMFCSLQCTGVTHLDLGENSLE
                     LMTAEELAAIFGVFKDSRLIHLGLNDSYLNNRTTEDLHLIFNALPANIATIKLSLEEL
                     TEMSSEQRQTIQRRFPRAEQVILVNNVEGRELNSSLSLGDANVYRRLGFVGVSPPSLA
                     GLTSFFLVGNNLRLTREVQKLLPDEVTERIVKNILG"
     gene            2347..3771
                     /locus_tag="U6309_002584"
     CDS             2347..3771
                     /locus_tag="U6309_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEN5506006.1"
                     /translation="MIVIFVHGWSVTHTNTYGELPQWLEKQSKQGKLDIQVGNIYLGR
                     YISFDDTVTVDDIARAFDQAVRDEIADKLGDGQRFACITHSTGGPIVRKWMDLYFKNN
                     LAKCPLSHLIMLAPANHGSALAQLGKSRLGRIKSFFEGIEPGKCVLDWLELGSDMSWQ
                     LNESWLDYDCTANGVYSFVLTGQKIDRQLYDAVNSYTGESGSDGVVRVAATNMNYSLL
                     KLHQEGDNGESLVVAKMTRTQPMAFGVLPGLSHSGKNIGIIRSITMANAATHPTAIWV
                     LRCLQVKSRDSYNKLVKELDNITKETQKNEHKEFVKTLVFTREYITNRYSMIIFRLID
                     DRGNHLIDYDLYLTAGPQYSEQALPAGFFVDRQRNLNNRGKLTYFLDYDIMEGGINTP
                     KMQGNLGFRVKAYPESSDQALAYYRLLDFHSSLADIHKILHPNETVMVEIMLQRRVDR
                     TVFRINNNLAPAKISGKPTGKKID"
     gene            complement(3844..4521)
                     /locus_tag="U6309_002585"
     CDS             complement(3844..4521)
                     /locus_tag="U6309_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEN5506007.1"
                     /translation="MYSYIDFNDKAHRLIPSKFPPVTLFDWADSAEELEQIALLEGLT
                     NERILAEFGKINLIDQEDWIGGPGSTPIMAAFTHTGFESRFSDGSFGIYYAASSLETA
                     IKETCFHRERFYSASNEKPCSISMREYVANIKKPLIDITDKSYKDLFNPDPACYSKSQ
                     EFGKKICEKKEWGLLYPSVRDLNGLCMAVLRPPALTIPIQGCHLRYIWDGEKISDIYK
                     ESKITNI"
     gene            complement(4523..4897)
                     /locus_tag="U6309_002586"
     CDS             complement(4523..4897)
                     /locus_tag="U6309_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HEN5506008.1"
                     /translation="MQTNTRSLQSIPDEVVWKSLTNLVERFDLKESEARILMGDMPRS
                     TYTSHKAKLNRDQKERVSYLLGIYKSLRILFEDGEQARTWINRKNDLPPFKGLTPKEY
                     MLEGGMVRLADVRKFLDFWRGY"
     gene            5110..>5341
                     /locus_tag="U6309_002587"
     CDS             5110..>5341
                     /locus_tag="U6309_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEN5506009.1"
                     /translation="MIDTIQSEPKINFTYNYKQPDDYRFSLDSIHLAKFVAKQLESYP
                     DLGSLRVLDLCAGCGVIGIELSWYLQAIRQIDF"
BASE COUNT         1657 a         1074 c          975 g         1635 t
ORIGIN      
        1 cttcaatttt taattcttgt aaaatgctct tatctgataa gatatatata aaatcagaac
       61 agtaaatgga tagatcgtct gatattttgg aaaataccat aattaatccc atacaatatt
      121 tcccaaattc atctaaggtg acgttgtgat tttcatggct tattgaatag ttataaagag
      181 cgaccatcac accaaaaata aattctggct caaaattttc tccaaaataa gagttttctt
      241 ttaataattt gaataaatgg cttataaata acctggcttt ttctttgttt gatgggcaaa
      301 ttgtttcacc taaatattcc aatagagtgt cataccacat aataatcctt taaatccctt
      361 taataggtag tgtcataaca taaatacatt taaaaaaaaa tctgctgata tttttatatg
      421 cattaatgat atttattatg ctcaattttg aatttcatca ctttatgcaa tgagaattat
      481 tgacgcatgt taaatacctg atatactccg ctaccattga ttggagagat ggccgagtgg
      541 tcgaaggcgc tcccctgcta agggagtatg ggagaaatcc catcgagggt tcgaatccct
      601 ctctctccgc caatcaacgc gcccgtagct cagttggata gagtatctgg ctacgaacca
      661 gggggtcgga ggttcgaatc cttccgggcg cgccatttaa aatttcatac gagcctattc
      721 atccgagaat attcttgaca atacgttctg ttacttcatc cgggagtaac ttttgcactt
      781 cacgtgtgag cctgaggttg ttgccgacca aaaaaaagga agttaaacca gctaaagacg
      841 ggggcgatac cccaacaaag cctaaccgcc gatatacatt ggcatcgccc agacttaatg
      901 aagagtttaa ctccctaccc tccacattat taactagaat aacttgctcc gccctgggaa
      961 aacgcctttg aatcgtctga cgttgctcgc tgctcatctc agttaactcc tcaagcgata
     1021 atttaatcgt cgctatattg gctggcaatg cattaaaaat aagatgcaaa tcttctgttg
     1081 ttctgttatt taaatagcta tcatttaaac caagatgaat taatctggag tctttaaaga
     1141 ctccgaatat agctgccaac tcctcagcgg tcatcaactc cagactattc tcgcctaaat
     1201 caagatgagt cacccctgta cactggagtg aacaaaacat cgctgtcatt tcaaacatgt
     1261 ccagataccc aatgccatta cggcataaat tgagatgggt gacggaggtg tcttttagag
     1321 catcaaatag agtctcaaaa gcagtaacaa ccattagatg caatccatta tcacttaaat
     1381 cgaggcgagt gattggagag tcttttatgg cactcaacag ggatgaaaat tcggcacctg
     1441 tcctttgata aagatcatta ttacgtaaac caagatggct aattgaagat actttcaatg
     1501 cacggaacat ttccgacaac acgatccccg attttttacc aagatcatta ttggtcaagt
     1561 caagatcaat tattggagta ctactccatg ccttaaatag ttcgattagt tcagcgtctg
     1621 gtctttgtcc cagccaatta cagcttaaat caagctgaat gatatctgaa tcgcttaaaa
     1681 cggttaacaa ggtggctaac tccgtcccgg tcttttgata aagctcatta gcacttaaac
     1741 taagacggct gattccagag tccttaagaa tcttaaacag agctaccaat tcagttccag
     1801 tcttttgccc aagattattg aaatgcaaat taagataagt gactccaaaa gccttgagca
     1861 tactgagcag aactgccaag tcagtgaatc tcttctcact cagtcggttt tttgacaaat
     1921 tgagttggac aatccacaag ccttttagtt ggttcaattt gacaatgatt tttttaatgg
     1981 atggattaaa tagttcaacg cgcatggatg cttcttatat tactttgcat ggctaaaaaa
     2041 agcagattcc agctgattta caaaagctac gtcttttcac cttaatctgt cccaatcttt
     2101 ctcgccgtgc aatattgtac tatatcaccc atccatatcc ataatatctt atggactata
     2161 attttcaaaa cttctaagcc gcataagcag tcacagtagg caaaatattc attaaaaggc
     2221 tgcttatttt taacactgaa aattctgggg ccataattaa aaacttgagg attgagtata
     2281 aaataatcta atatcatcaa gttactcaac aatgtcttgt tgtgagccaa cgattcaagg
     2341 agcgttatga ttgttatctt cgtccatggc tggagtgtca cgcataccaa tacttatggg
     2401 gaactacctc aatggcttga aaagcagagc aaacagggaa agctggatat tcaagtcggc
     2461 aatatttatc ttgggcgcta catcagtttt gatgatacgg tgacggttga cgatatagca
     2521 cgcgcatttg atcaagcggt acgcgatgaa attgctgata agctaggaga tgggcagcgt
     2581 tttgcttgta tcactcactc taccggtggg cccattgtcc gcaaatggat ggatttatac
     2641 tttaaaaata atcttgcaaa atgtccgttg agtcacctta tcatgctggc tcctgccaac
     2701 cacggttctg ctcttgctca gcttggtaaa tcccgactag gccgcattaa gagttttttt
     2761 gaaggcattg aaccgggaaa atgtgtgctt gattggcttg aactcggaag cgatatgagc
     2821 tggcaactta atgaaagctg gcttgattat gattgtactg ccaatggtgt ctattccttt
     2881 gtacttactg gccagaaaat tgatcgccaa ctttatgatg cagtaaactc ctacacagga
     2941 gaatctggat ccgatggggt ggtacgcgtt gctgcaacaa atatgaatta tagcttattg
     3001 aaattacatc aggaggggga taacggtgaa agtcttgttg tcgccaaaat gacacgaaca
     3061 cagccaatgg catttggagt tctacctgga ctttcacatt ccggtaaaaa tatcggtatc
     3121 atccgtagta ttactatggc caatgcagcc actcacccca cggcgatatg ggtcctgcga
     3181 tgcctgcaag tcaaaagtcg tgactcctat aataaattag taaaagagtt ggacaatatt
     3241 actaaagaga ctcagaaaaa tgagcataag gaatttgtga aaacacttgt ctttacgcgc
     3301 gagtatataa ccaatcgtta ctctatgatt attttccggc ttattgatga ccgcggcaat
     3361 caccttatcg attatgatct ttacctaact gccggacctc aatatagcga gcaggcgctt
     3421 cctgcaggtt tttttgtaga tcgtcagcga aatctcaata atcgaggtaa actgacttat
     3481 tttcttgact acgatatcat ggaaggtgga attaataccc caaaaatgca gggtaatctt
     3541 gggttccgtg ttaaagcata tcctgaatca agtgaccaag cccttgccta ttacaggtta
     3601 cttgattttc attcatcgct tgctgacatt cacaaaatac ttcatccgaa tgaaaccgta
     3661 atggttgaaa tcatgcttca aagacgagta gataggacag ttttccgtat caacaacaac
     3721 cttgctccgg caaaaatcag tggaaagcca actggcaaaa agatagattg atatcttttt
     3781 cgtttgatgc atttgcatca ttaatgagaa agtcatctag ccacaatttg gaaaaaaaat
     3841 atcttaaata ttggtaattt tactttcttt ataaatatct gaaatttttt caccatccca
     3901 aatatatctt aaatggcatc cttgtatcgg tatagtgagc gcgggaggtc ttaatacagc
     3961 catacataag ccatttaaat ctcttacact tgggtatagc aagccccatt ccttcttttc
     4021 acaaattttc ttgccaaatt cctggctttt actataacaa gcaggatcag gattaaacaa
     4081 gtctttataa cttttatctg taatatctat taatggtttt tttatattag ccacatactc
     4141 tctcattgag atggagcaag gtttttcatt tgatgcgcta taaaaccgtt ctcgatgaaa
     4201 gcaagtttcc tttattgctg tttcaagtga tgaagcggca taataaatac caaaactgcc
     4261 atcgctaaat cttgattcaa atccagtatg tgtaaaagca gccataattg gagtagatcc
     4321 aggaccacca atccaatcct cttgatctat caaatttatt ttaccgaact ctgcaagtat
     4381 cctttcattg gttaagcctt caagtagggc tatttgttcg agctcttctg cagaatctgc
     4441 ccaatcgaat aaagtcacgg gagggaattt agaaggtatt agtctatgcg ctttatcatt
     4501 aaaatctata taggaataca tcctaataac ctcgccaaaa gtccagaaat ttccttacat
     4561 ctgcaagacg aaccatcccc ccttccagca tatattcttt gggtgttaaa cctttaaacg
     4621 gaggtaagtc gtttttacga ttaatccagg ttctggcttg ttccccatct tcgaacaata
     4681 tacgtaaaga cttataaatt cctaaaagat aggagactcg ttctttttga tccctgttta
     4741 gtttcgcttt atgggaagtg tacgtagagc gtggcatatc ccccatcaaa atacgggctt
     4801 cactttcttt taaatcaaaa cgttctacaa gattggttaa agacttccat accacctcat
     4861 ctggtatgct ttgtaaagac cttgtattag tttgcatact aaactcctgg ctgctttcta
     4921 ctatattata gtctccaaag agacaattgt ctataaaaag acatgttaag tgaattaaat
     4981 gctcatttaa tgaaaaatac attgatctga catgcagaat atggaataaa tttcaaaaat
     5041 taattaacag tgcaacaagc aagagaataa ggtgctttaa aaacatagtt gagcgaaaat
     5101 aatttcaata tgattgatac aatacagagc gaaccaaaga taaattttac ttataattac
     5161 aaacaacctg acgactaccg tttcagtctt gattcaattc atctggctaa atttgttgca
     5221 aaacaattgg aatcctatcc agatcttggt tcattaagag tattagatct ctgcgcaggc
     5281 tgtggagtaa ttggaattga actgtcatgg tatcttcaag caatccggca gattgatttt
     5341 a
//
LOCUS       DASLOE010000183         5338 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.184, whole genome shotgun sequence.
ACCESSION   DASLOE010000183 DASLOE010000000
VERSION     DASLOE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.184"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            157..849
                     /locus_tag="U6309_002588"
     CDS             157..849
                     /locus_tag="U6309_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEN5506010.1"
                     /translation="MTMKFPFQIIDLTHPVSPDSPGWDLDCGFTHSNFIDYEDCTSEI
                     KFRVQQIHSPLGIGTHMDAPAHCIPGAASITDLDLHRNYLLTQSVLINVSHQTDEHYQ
                     ITKQDILDFEARYGRLKKNVFVLFYTGWEKHWPNPDKYRNNLVFPSISAHAAQLLLER
                     NIAGIGIDTLSPDTADSGYPVHQLLLQAGKYIIENVANANSLPPIGAWTLALPWKIIN
                     ATESPVQLIGLV"
     gene            complement(900..2318)
                     /locus_tag="U6309_002589"
     CDS             complement(900..2318)
                     /locus_tag="U6309_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homospermidine synthase"
                     /protein_id="HEN5506011.1"
                     /translation="MDKNHNTKKILFKNRFVLLGFGCVGQALMPLIFEKFDIKPSQVT
                     IIADEGARVEVAQQYGVSFKLQQITPQNYLEVIGGTLEENDFLIDVSIGISSLALIIL
                     CNQKGALYINAATEPWKEEFVMEKMALNRRTNYSLREEVLHLKDKTQKTALITHGANP
                     GLVSHFIKEALLNIAKDNGLTINRPKNAAEWANLAMTLGIKVIHVAEQDSQVTYPPKS
                     PGEFVNTWSANGLILEGLQPAEIGWGTHEAHWPHDAYSHSNGPQCAIYLSRPSAGVMV
                     RSWTPTLGAFHGFLITHAETISLTNFLTLKNGSELLYRPTVHYAYNPCPDARLSIFEL
                     KSNEWKPQNKNRLILNEIIDGCDELGVLLMGNQRGAYWYGSTLSIQEARQIAPYNNAT
                     SLQVVASMISGIIWAIEHPDEGIVEPEEVDHQYIIDIAKPYLGKVGGYYTDWTPLKNR
                     GELYPEEVDLSDPWQFFNIRVS"
     gene            2596..3045
                     /gene="trmL"
                     /locus_tag="U6309_002590"
     CDS             2596..3045
                     /gene="trmL"
                     /locus_tag="U6309_002590"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEN5506012.1"
                     /translation="MIHIALYQPEIPPNTGNIIRLCANSGAQLHLIHPLGFTLEDKQM
                     RRAGLDYHQWASIFHYENWQTFLQSHSQRRLFCCSAKGKVKYSDVDYKADDILLFGPE
                     SCGLPQQILNQYSTIRIPMRENNRSLNLSNAVAIILYEAWRQMDFNM"
     gene            complement(3204..3854)
                     /locus_tag="U6309_002591"
     CDS             complement(3204..3854)
                     /locus_tag="U6309_002591"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEN5506013.1"
                     /translation="MLDVINFKDYRSILCLNGDLPAPSFFHKRKLPVIAADGAANVLC
                     NLGVFPDLITGDLDSIQPALLENHSFLHLPDQGSTDYQKAMHYLQTHDLLPAIVVGIN
                     GGYLDHILNNINIFMDTNCLLYSPPIKGFVVNKKLRVNFILPAQTKISLIGIPEVVLS
                     SEGLKWELKHSHLSFPGKNSCFNRTQTAEISLEVHQGAALVLIYEDAIEDAGVSSL"
     gene            complement(3838..4104)
                     /locus_tag="U6309_002592"
     CDS             complement(3838..4104)
                     /locus_tag="U6309_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506014.1"
                     /translation="MSIVIFSGIVAFITSFIGLLPQIVKSLKTKSTEDLSMMMLVNYL
                     VCSLAWIIYGSSTNSFFVTSSNVVGLIVSTLLILLKRRYDARCN"
     regulatory      complement(4091..4194)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4336..5265
                     /locus_tag="U6309_002593"
     CDS             4336..5265
                     /locus_tag="U6309_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEN5506015.1"
                     /translation="MTITHTAAGGITMTVELIRNGETDTNDYEEHLDSFGYETLEKSL
                     KEDAKSICQFFCNEELVLQGLKKLVTQFYQFNQPEKKTLRQFFDDWGSKNHFNHEAPP
                     NDKIPYETSPNFHFPNHTPILHGELTADLFNNVLLKQGYLSADLGAGPKHGKWAHTIQ
                     FFILEEARKTGQLKLHSNTVCEFIKKISQIHGQYESFSLWSILFDSFDETFTCPNIIT
                     QTLTSPWDNSKEALFLADKLNHFQKKFEKAASAENNYNAYSNKKYMSRIDKAHYVKYK
                     DKCAILWFAPQDKKTTPKPSCQEELKQNQLSLL"
BASE COUNT         1788 a         1076 c          915 g         1559 t
ORIGIN      
        1 aatttgaata aaattgttaa ttattagctt gaatgatcat tattataata aaaagacaaa
       61 ctgacaatct tttgcaaaac ctaaattaaa aattagtttc attgaccctg attcttttta
      121 tgataattta attgaaataa cccatcgctc tcccaaatga ccatgaaatt tccttttcaa
      181 ataatcgatt taacacaccc tgtttcacct gactcccctg gctgggatct ggattgtgga
      241 tttacccata gtaattttat tgattatgaa gactgtacat cggaaatcaa attcagagtt
      301 cagcaaatac actcaccctt ggggattgga acccatatgg atgctccggc tcattgtatt
      361 ccaggggccg cctctattac tgatctcgat ctgcaccgta attacctgct cacacaaagt
      421 gtgctgatta atgttagtca tcaaacggat gaacattatc aaattacaaa acaggatatt
      481 ctggatttcg aagccagata tgggaggtta aaaaaaaatg tgtttgtgct tttttatact
      541 ggctgggaga aacattggcc taatcctgac aaatatcgca acaatttggt ttttccaagc
      601 attagtgccc acgcagccca attattgctt gaaagaaata ttgctggcat tgggatagac
      661 acactttctc cagatactgc agactcaggt taccctgtgc atcaactctt attacaagca
      721 ggtaaatata ttattgaaaa cgttgccaat gcgaattccc tgccacccat tggcgcctgg
      781 accctggctt taccatggaa aataatcaat gctacggagt caccagtcca attaattgga
      841 ctggtatgat aatgtatagt tcattgatta atactaatta ttacagtacg ttactccttt
      901 taactaaccc ttatattgaa aaattgccag ggatcagata agtccacttc ttcaggataa
      961 agttcccctc tattttttaa aggggtccaa tccgtgtaat agccgccgac ttttccaaga
     1021 tacggtttgg caatatcaat aatatattgg tgatcaactt cctctggttc tactataccc
     1081 tcatcaggat gctctattgc ccagattatt cctgaaatca tactggcgac aacttgtaaa
     1141 ctggttgcat tattataggg ggcaatttgc cttgcttcct ggatagaaag agttgaacca
     1201 taccaatacg cccctctttg attacccatc aacaacacac ccaattcatc acatccatca
     1261 atgatttcgt ttaaaatcag gcgattcttg ttttgtggtt tccattcatt actttttaat
     1321 tcgaaaatgg agagcctggc atctggacaa ggattatagg catagtgaac tgttggccta
     1381 tataataatt cagatccatt ttttaatgtt aaaaaattgg taagtgaaat agtttctgca
     1441 tgagtaatta aaaacccgtg aaaagcaccc agtgttggcg tccatgaacg taccattaca
     1501 ccggcactag gacgagacag atagatagcg cattgtggtc cattactatg agaataagcg
     1561 tcatggggcc aatgagcttc atgtgtcccc cacccgattt cagcaggttg caatccttcc
     1621 aaaatcagac cattagcgga ccaggtatta acgaactcgc ctggtgattt tggaggatag
     1681 gtaacttgag agtcttgctc tgcaacatgg attactttaa ttcccaaagt catggcgaga
     1741 ttagcccatt ctgccgcatt tttaggcctg ttaatagtaa ggccattatc ctttgcaata
     1801 ttcaacaagg cctctttaat aaaatgtgaa acgagacccg gattagctcc gtgagtaatt
     1861 aaggctgttt tttgagtttt atcctttaag tgaaggactt cttcacgcaa agaataattg
     1921 gttcggcgat taagcgccat cttctccata acaaattctt ctttccacgg ctcagttgca
     1981 gcattaatat acaatgctcc tttttgatta cataatataa tcagagcaag acttgatatg
     2041 ccgattgaca catcaattaa aaaatcgttt tcttccaaag taccaccaat cacttcaaga
     2101 taattttggg gagtaatttg ttgtaacttg aatgaaactc catattgttg agcaacctca
     2161 acccttgccc cttcatcggc aatgattgtc acttgcgatg gtttgatatc aaatttctca
     2221 aagattaaag gcattaatgc ctgaccaacg caaccaaatc ctaataagac aaaacgattt
     2281 ttaaacaaga ttttttttgt attatgattc ttatccatga tgaatcctta acttcccggc
     2341 ccagtttaat ttgaattttt aatactcaac aactattcaa caatgatcat acccaacaga
     2401 catcaaaaaa tttcatatgg catactaata ttgtagtagc tatgttgaat tcagacagcc
     2461 tgttgagtaa aaaattagac atatcaactc cctgcttgat ttttaaagta tgtagtaaaa
     2521 ttgcatcctg ccactctatt cctggctttg aataaatcaa ttaaaatggt ggccagaatg
     2581 ctactggaaa atcacatgat ccatattgcg ctttatcaac cagaaattcc accgaacacg
     2641 ggcaatatta ttcgactctg cgctaatagt ggagctcaac tgcatttgat tcatccatta
     2701 ggctttactt tggaagataa acaaatgcga cgagccggtc ttgattatca tcaatgggcc
     2761 tcaatattcc attatgagaa ttggcaaaca ttcctgcagt ctcattcaca acgacgatta
     2821 ttttgctgta gcgcaaaagg aaaagtgaag tatagtgacg tcgattataa ggcagatgac
     2881 atcttattat ttggacccga atcttgcggc ttacctcaac aaatactgaa ccaatattca
     2941 acaatacgga ttcccatgag agaaaataat cgtagtctca acttatcaaa tgctgtagca
     3001 atcatattgt acgaagcatg gagacaaatg gatttcaaca tgtaagcatt aactaggtaa
     3061 tccacctgat agtgattaca gcaatcgaag agacaggtta tgcaagcagt actctaatat
     3121 aaacaggact tacgaaaaac cacaaggccg aggcaaaaat attttttaac aaagagattg
     3181 gggagggcgt aagtccttat tccttataaa gaggaaactc cagcatcctc aatagcatct
     3241 tcataaatca aaacaagggc agcgccctgg tgaacctcca aactaatttc tgctgtttgt
     3301 gttcgattga aacaggaatt ttttccagga aacgaaaggt gtgagtgctt tagctcccac
     3361 ttcaaaccct cagaagacag gacaacctca gggataccta ttaacgagat tttagtctgg
     3421 gcaggtaaaa taaaattaac cctcaatttt ttattgacaa caaaaccttt gatgggaggc
     3481 gaatataaaa ggcagtttgt atccataaaa atgttgatgt tattcagaat gtgatccaaa
     3541 tacccaccat ttatccctac aacgatagca ggcaatagat catgagtctg taaataatgc
     3601 atggcctttt gataatccgt actcccttga tctggtaaat gaagaaaaga atggttttcc
     3661 aacaaagcag gctgaataga atccagatct ccagtaatta agtcaggaaa gacaccaagg
     3721 ttacataaca cattagcggc accatcagca gcaataaccg gcagctttct tttatgaaaa
     3781 aaagaaggag cgggcaaatc accattaaga cataaaatag aacgatagtc tttaaagtta
     3841 attacatcga gcatcataac gtctttttaa taaaatcagc agggtactca ctatcaaacc
     3901 cactacattg gaactcgtta caaaaaatga gtttgtgctg cttccataaa tgatccaggc
     3961 cagcgaacaa actagataat tcactaacat catcatggaa agatcttcag ttgatttggt
     4021 ttttaaggat ttaacgattt gcggtaaaag tccaataaac gaggtaataa aagcaactat
     4081 tccagaaaaa ataacaattg acacattttc ctccgctcag actaatgaga tcaggttcaa
     4141 agggttgggt gatcctctca gccaacttat caattggcac ccctaatgtt ggacttatat
     4201 tttatcacga aatgagcaga ccgcaattta atagcaagaa aatactcctt tctttttcca
     4261 tcagtttatg cttaattaat aatcctttag tccaaaaaag gaattaagcc cctcaaataa
     4321 ttctcaaatc ggtatttgac aataactcat acagcagctg gaggcatcac tatgacagta
     4381 gaacttattc gaaatggaga gacagatacc aatgattatg aagaacacct ggattctttt
     4441 ggatacgaaa cattagaaaa atccttgaaa gaagatgcca agtctatatg ccaattcttt
     4501 tgtaatgaag agctggtttt acaaggttta aaaaaattgg tgacacaatt ttatcagttt
     4561 aaccaacctg agaaaaaaac attaagacaa ttttttgatg actggggaag taaaaatcat
     4621 tttaaccacg aagctccgcc taatgataag ataccttatg aaaccagccc caattttcat
     4681 ttccctaatc acactccgat tttacatggt gaattaactg cagacttatt caataatgtt
     4741 cttctaaaac agggttactt atctgctgat cttggtgcag gccccaagca tgggaaatgg
     4801 gcgcatacca tccaattttt tattctggaa gaagccagaa aaaccggaca attaaagctt
     4861 cacagcaata cagtctgcga attcattaaa aaaatctctc aaatccatgg ccaatacgaa
     4921 tcattcagtt tatggagcat tctctttgat tcatttgacg aaacctttac ctgtcccaat
     4981 ataattactc aaactttgac atcaccatgg gataattcga aagaggctct atttttagct
     5041 gataaattaa accatttcca aaaaaaattt gaaaaagcag cctctgctga aaataattac
     5101 aatgcgtact ccaataaaaa atacatgagc agaattgata aagcgcacta tgttaaatac
     5161 aaagacaaat gcgcaatact atggtttgcg ccacaggata aaaaaactac cccaaaacct
     5221 tcctgccaag aagagttgaa acaaaatcaa ttaagcttat tataagagga tatgacttta
     5281 tatcgataaa cttaaataaa acatccctga cttggatagc aactctttac caaggcag
//
LOCUS       DASLOE010000184         5325 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.185, whole genome shotgun sequence.
ACCESSION   DASLOE010000184 DASLOE010000000
VERSION     DASLOE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.185"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..302
                     /locus_tag="U6309_002594"
     CDS             <1..302
                     /locus_tag="U6309_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506016.1"
                     /translation="IEDITPQDVEKFFNQCSQYGSEAAEKLADNRPVLTKIADILTAI
                     ARWAISLIGFNTPPQFLAPTRTCVDQVSDEITKIKLKLEDTLGSLRKAQEESLSL"
     gene            671..1264
                     /locus_tag="U6309_002595"
     CDS             671..1264
                     /locus_tag="U6309_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506017.1"
                     /translation="MLPFKYGYMSGVDIMTRSNSEQLEEEYLNSRREYCIAMMAVHDK
                     ARALLSETPIDGSMKQAIIKSSGVKIRPNETQDEFDERFEDEMGLTIDDYVGFALDIL
                     EKKLEPARQSIVAAANKELGSLTSGPSGSAWEDFQHTIHEGAEYKTIEVAKCLPESER
                     CIQAFNQAKLAFRDAATNLIHAEKLEEPEEPNQGPRF"
     gene            complement(1410..3287)
                     /locus_tag="U6309_002596"
     CDS             complement(1410..3287)
                     /locus_tag="U6309_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN5506018.1"
                     /translation="MVFIMQKIIILTKKIIAKLPVILFDVAAIPVAWYAAYWLRYNMQ
                     PYPSSLTSTHSFIALALLSIVQISCYYYFKIYRGLWRFSSLNDVIRILKATITAMVLV
                     IPVFYLTSILQHLPRSVFPLYCIILATILCGGRLVIRLHWDKPGRGNRELETKRVLVV
                     GAGQAGEGLVRDLKRSSYYRPIGFVDDNKSKRGLEVHGVRVLGTTNQITELVNQYDVD
                     LIFIAIPSAKSATMRRIVTLCEQSHVPFSTLPSISALAAGRVEVNALRPVNIEDLLGR
                     DQVTLEWEKIARSIAGRRILITGGGGSIGSELCRQVMALEPASIAIVENSEFNLYRIE
                     QELLKSFPGIPVELSLISVTDEIAINHLFRRFQPEIVFHAAAYKHVPMLQHQVRVAVF
                     NNVIGTQVVAKASVTFGAEKFILISTDKAVNPTNIMGTTKRVAEIYCQNLNTRVKTQF
                     ITVRFGNVLGSAGSVVPLFQKQLQEGGPLTVTHPDMQRYFMTIPEACQLILQAMVNGE
                     GGEIFVLDMGEPVKISYLAEQMIRLAGKEPGRDIMIEYTGLRPGEKMYEELFHETEQL
                     TSTKHEKLFKARFRELDWNDLTQTMRMLQAACIEHQDEELLVLLKSLVPEFQLEPMVE
                     A"
     gene            complement(3342..4889)
                     /locus_tag="U6309_002597"
     CDS             complement(3342..4889)
                     /locus_tag="U6309_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein LphB"
                     /protein_id="HEN5506019.1"
                     /translation="MVLRYPDIHGIELMSAKWRWYDSLFILLFAYLLVLQIQAIWLFT
                     IDDMYISLRYAKNWAAGNGLLWNLNAEPVEGYSNFSFVALGAISIILHINPVLTLKIA
                     GFAGLLATCLFIFLISRFWFTKRESFIPCIWLLLYKGQVIWSVSGLETTVYQALISGS
                     VYFAFRGLGYHFYPAPRGEFKTSGFVLSGLYLSFAGMTRPETPALMLLFFLLIGWDWN
                     KLSGKKPWRGAVSFAVTIGLVFLPYFLWRWSYYGLLFPNSVYCKGFNGISYSLDLNYL
                     KLIWPFAILALPACFYAQDRRHYFLWSPGIIYLLMLVNADPIVAFDNRLFLPAFALML
                     PLTLNGISYLLLSYFKKQDNYFRAALYVSSFFLALLFIPKMSLDAYRYFSQNPVHGEQ
                     LRKSVVQWLNTHAKNQDRVVLADSGLIPFLSGLTFIDSYCLNNKEMAQFPAVKRYELF
                     CQQILEEKPQIIILTALIDKGKIIYTPGDSCLKPLLDVHKEYKLGGVFSFGSAESQYR
                     YELFANF"
     gene            complement(4850..>5325)
                     /locus_tag="U6309_002598"
     CDS             complement(4850..>5325)
                     /locus_tag="U6309_002598"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEN5506020.1"
                     /translation="LKLIPVFDVACIALGFMLRVLAGTIGIDLAISAWLTVAATLLSL
                     FIALNKRQLEMQLGLKNSTRKVLRRYNPVFLQRLIVITGIACFITYLFYTVYARAESF
                     FFILTLPFAAIALWRFAWLATQDVENDDPVNIFLSDRLSRINLWCFAILTFMALN"
BASE COUNT         1814 a         1139 c          973 g         1399 t
ORIGIN      
        1 tgattgaaga cattactcca caggatgtag agaaattttt taatcaatgt agtcaatacg
       61 gttcagaagc agctgaaaag ttagctgaca atcgcccggt actgacaaaa attgctgata
      121 ttttgactgc cattgcccgt tgggcgatta gtttaatagg tttcaacacg ccaccgcagt
      181 ttttggctcc aaccagaaca tgtgtggatc aggtatctga tgaaatcact aaaattaaac
      241 tcaaacttga agatactttg ggaagcttgc gaaaagcaca ggaagaaagc ctaagtcttt
      301 aataacgatt aagtccacat taaaaaaata tttgatataa gaatttacct gttatcagat
      361 atatcctccc ggattacagc tttcctgtaa tccgaggcca ctttacgatt agattatgca
      421 tcatatacct actaatgagt ctggtaggat tagcacagat tttacccctc cctctcctca
      481 acaatcttcc gcgaaaagca tcatcatacc acctaagccc ctggatcgag agagagagca
      541 gatcgaagtg aaattaaaat acccactcaa tttaagatca aatattcaat aaaaaatcag
      601 ggatattgac ttagtatgca ccacgacctt gatggtatta ttattgtctt tttgaataat
      661 tataaatcaa atgctacctt ttaaatatgg gtatatgagt ggggtagata tcatgacaag
      721 gtcaaatagt gaacaactgg aggaagaata ccttaactca aggagggagt attgtatagc
      781 aatgatggcc gtacacgata aagcaagggc attactgagt gaaactccaa tagatggaag
      841 tatgaagcaa gctataataa aatcaagcgg ggtaaagatc cggcctaacg agacacaaga
      901 cgaattcgat gagcgtttcg aggacgaaat gggtttaacg atagatgact acgttggttt
      961 tgcgcttgat atattagaaa aaaaattgga gcctgcgaga cagagtattg tagctgcagc
     1021 aaataaagaa cttggttctc ttacctctgg accatctggt tcggcatggg aagattttca
     1081 acacacaatc cacgagggag cagaatataa aactatagaa gtggctaagt gtctccctga
     1141 gtctgagcgt tgcatacaag cttttaatca agctaagctg gcatttcgtg atgcagccac
     1201 aaacttaata catgcagaga aactggaaga accagaagag ccgaatcaag gccccagatt
     1261 ctaaaaccag gacacaggaa gaagcaagaa gctaaaaaat tcttttgctg tttttcattt
     1321 taccatcaaa tgatgagaaa atagagtttc aaaatcaaca taagtaattg ccataacaca
     1381 atcttgattt accagattag ggatattgat taagcttcaa ccataggttc caattgaaac
     1441 tccggcacca gactttttaa cagaacaagc aactcctcat cctgatgctc aatacaagct
     1501 gcctgtaaca ttcgcatggt ttgtgtcaaa tcattccaat ccagttcacg aaaccttgct
     1561 ttgaataatt tttcatgttt tgttgaagtt aactgttcag tctcatgaaa caactcttca
     1621 tacatttttt cgccaggacg caaacctgta tattcaatca tgatgtctct tcctggttct
     1681 ttgcctgcca agcgaatcat ttgctctgca agatagctga ttttaaccgg ctctcccata
     1741 tccaggacaa aaatttcacc accctcccca ttcaccatcg cttgcaaaat caactggcat
     1801 gcttccggta tagtcataaa atatctttgc atatcgggat gggtcacagt cagtggacca
     1861 ccttcctgca actgcttttg gaacaaaggc accacacttc cagctgaacc cagaacattt
     1921 ccaaaacgaa ccgtaataaa ctgggtttta actcgtgtat ttaaattctg acaataaatt
     1981 tcagcgactc tcttggtagt tcccattata ttcgttggat tcactgcttt atcagtggaa
     2041 attaaaatga atttctcagc tccgaatgtc acactggctt tggcaacaac ctgagtacct
     2101 atgacattat taaacacagc aacccgtact tgatgctgaa gcatgggaac atgtttgtaa
     2161 gcagccgcat gaaatacaat ttcaggttga aaccgacgaa ataagtgatt aatcgcaatt
     2221 tcgtctgtca cactgattaa actcaattca acggggatac ctggaaaact ctttaacaac
     2281 tcctgttcaa tgcgatacag gttaaactca ctattctcaa caatggctat gctcgcaggc
     2341 tctaaagcca taacctgacg gcacaattcc gaaccgattg agccaccacc gcctgtaatc
     2401 aatatgcgtc ttcctgctat tgaacgagca attttttccc attcgagcgt cacttgatcc
     2461 cgacccaata aatcctcaat attcacaggc ctcagcgcat tcacttcaac ccggcccgca
     2521 gccagggctg aaatactggg taaagtacta aaaggcacat ggctttgttc acaaagcgtt
     2581 acaattcttc gcatggttgc cgatttggca gaaggtatgg caatgaaaat caaatccacg
     2641 tcgtactgat tgactaattc ggtaatttgg ttagtcgttc ccagaacccg cactccatga
     2701 acttctaatc ctctcttgct tttattgtca tctacaaagc ctattggccg ataataactg
     2761 cttcttttca aatcacgcac caagccttca ccagcctgcc cggcaccaac caccaaaact
     2821 cttttcgttt ccaattccct gtttcctctg ccaggcttat cccaatgcag acggatcacc
     2881 aaccttcccc cgcaaaggat ggtagccagg ataatgcaat acaaaggaaa aactgatctg
     2941 ggcaggtgct gaagaattga agtcaagtaa aaaacaggga ttactaaaac cattgctgtg
     3001 atagtagcct tcagtatcct gatgacatca ttcagagaag agaaacgcca taatcctctg
     3061 taaattttaa aataatagta acaactaatc tgaacaattg ataataaagc taaagcaata
     3121 aaagaatggg tggatgtcag gctgctggga tatggctgca tgttatagcg caaccagtaa
     3181 gcggcatacc aggcaactgg tattgcggcc acatcaaaca aaattactgg gagttttgca
     3241 atgatttttt ttgtcaatat gattattttt tgcattataa aaaccacagt ctgtaatcct
     3301 ttataaagga aagatggtac actatgttac agtttcccgc atcaaaaatt agcaaacaat
     3361 tcatacctat attgtgactc agcactgcca aaagaaaaaa caccacccaa cttgtattct
     3421 ttatgaacat caagtaatgg tttcaaacaa gaatcgccgg gagtataaat gatctttcct
     3481 ttatcaataa gcgcagtcaa aataattatc tgtggttttt cttctaaaat ctgctggcaa
     3541 aataattcat atctcttcac ggcaggaaat tgtgccattt ctttattatt caggcagtaa
     3601 gaatcaataa aagttaatcc gcttaaaaac ggaatcaaac cagaatctgc caacaccact
     3661 ctatcttgat tcttggcatg agtatttaac cattggacaa cacttttccg taattgctct
     3721 ccatgtaccg gattttgact aaagtagcga taagcatcaa gggacatttt gggtataaat
     3781 agtagcgcca gaaaaaatga actcacatac aatgccgctc taaagtaatt atcttgtttt
     3841 ttgaaatagc ttaataaaag ataactgata ccattcaagg tcagaggtaa cataagagca
     3901 aacgctggta agaacaatcg attatcaaaa gcgacaattg gatcggcatt cacaagcatt
     3961 aataaataaa taataccagg agaccataaa aaataatgcc ttctgtcctg agcataaaaa
     4021 caggctggca aagccagaat agcaaatggc caaattaatt tgagataatt caaatccaat
     4081 gaataggata tgccattaaa acctttacaa tatacagagt taggaaacaa taacccgtaa
     4141 taactccaac gccaaagaaa ataaggcaga aacaccaaac ctatggtcac cgcaaaagat
     4201 accgcccctc tccatggctt cttgcctgat aatttattcc aatcccaacc gattaaaaga
     4261 aaaaagagaa gcatcagggc tggtgtttca ggcctggtca ttcctgcgaa tgaaagatac
     4321 aacccggaca agacaaaacc acttgtctta aattcacctc gaggagcggg ataaaaatga
     4381 tatcctaacc cgcgaaaagc aaaatagact gaaccgctaa tcagtgcctg gtaaacggtc
     4441 gtttcaaggc cgctaactga ccaaataact tgtcctttgt atagtaaaag ccaaatgcag
     4501 gggataaaag actctcgttt ggtaaaccaa aagcggctga taagaaagat aaaaagacac
     4561 gtggcaagta atccagcaaa cccggcgatt tttagggtaa gaaccggatt aatgtgcaga
     4621 ataattgata ttgcccccaa agcaacaaag ctgaaattgg aataaccttc gaccggctca
     4681 gcgtttaaat tccataataa gccatttcct gcagcccagt ttttggcata ccgcaaagaa
     4741 atatacatgt catctatagt gaacagccat atggcctgga tttgtaaaac cagcaaataa
     4801 gcaaacaaaa ggataaacag gctgtcatac cagcgccact tagcactcat caattcaatg
     4861 ccatgaatgt caggatagcg aagcaccata aattgatccg agataatcga tcacttaaaa
     4921 aaatattgac cggatcatca ttctctacat cctgagtagc aagccaggca aaacgccata
     4981 gtgcaatagc agcaaaaggt aaggttaaaa tgaaaaaaaa cgattctgct ctggcataaa
     5041 ccgtgtaaaa cagataagta ataaaacagg ctattcctgt aatgacgata agtctttgca
     5101 gaaagactgg attatatcgg cgtagaacct tacgagttga atttttcaat cccaactgca
     5161 tttccagttg tcttttattt agggcgataa ataaactaag caaagtcgct gcaacagtta
     5221 accaggctga tatagccaaa tcaattccta ttgttccggc caatactcta agcataaatc
     5281 ctagggcaat acaagcgaca tcaaaaaccg gaatcaattt taaaa
//
LOCUS       DASLOE010000185         5209 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.186, whole genome shotgun sequence.
ACCESSION   DASLOE010000185 DASLOE010000000
VERSION     DASLOE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.186"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(96..2861)
                     /gene="ankF"
                     /locus_tag="U6309_002599"
     CDS             complement(96..2861)
                     /gene="ankF"
                     /locus_tag="U6309_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkF/LegA14/Ceg31"
                     /protein_id="HEN5506021.1"
                     /translation="MKDQLAKIFEKYPFVHLDKSGELSIIVPLTGAASISDNFTADET
                     KPIFLYLFGDNTCKSDLERSTFFYGKQKSGGRHSVKPFTELFSSLIHELNGLKSSTEI
                     RVDEQLFLETIIANLNELKEQALAFRKGNPGFEDMKNDFSHSDEIMVSKSEFVVTVYP
                     DRVRQFDLPNVMSLINPGVSRYKQLLAQSDINQCLEALSLVQKPAAEGNLDLIADALG
                     KLDADPIDYLCKKDNKDPQALFKAVYEILQKQDTSLENQQSAVFEVMEGNLVAAFGDN
                     EDFSTWQKEEIVDAIKSIFKQANLLSEQSGTKQKTFADVYNQAFDKYNESTNDDSESG
                     CKRRNTYQLFILQTFLYLCSLQLRLKNKEKAKKFLGLFEDRGTLNNLVLQLCNDEKAF
                     MRMMADEFELTSAEYASIAESTYGIVLAHIDAEHFDELRIACSAQLLQDSNYIVMGGR
                     LCWSTSSINEEGNIRSLEQQKKISSENFEIFYNQRQSYIDKSREFQQIKVLLDKEVND
                     EGIAEAVASKVRYLTKYQKSQILSEVIQKKKYEHAKLLIENYANLVFPSVFISVIKSR
                     PVHIDLIRAFLNADSRHALNIDGNDLIQAAKKGDFELVKIIIEHRPDLLESKDSFQQT
                     PLLWACVNGYVDIVEYLMDSGADIHVRTKVSGSHRYKIGETPTGGRTALDWARCYDKT
                     DPVKASKIIALFDTYYQRMEDHFKANSAYKDQFNKAHLTQAINNGDLKLIALFLKYCP
                     HLEKFFTQEDQLHAKQRHNQQMLRSQIMPYQNSFNKLLDELQRKAELLNKKYGTLHEA
                     TNVMQALINDLSTVRNDFFMDDITPQSFQKFERKCKQVLEEASPVLSTHRGWHGYPDF
                     VRRFIGVIATLAVIPALVVHFSSAKGYVGVFFSGKENIKTDSSEKMGEFANNLETLKT
                     EISNKLA"
     gene            3085..3579
                     /locus_tag="U6309_002600"
     CDS             3085..3579
                     /locus_tag="U6309_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506022.1"
                     /translation="MAFQRSFFSHIEDDWRYYQNIRSKYSDAIPIPQRKYFEPIHSID
                     SFATLAVRPIEKPLWLGVHTAGFLLKAIIHLVGALVLSPFALIFAVCVPRSELREQTV
                     SSFKSTAAGSIVAAGMACVALLSTLMSLIFNPLYALSRSAATGIDHLNSVTESCCGLT
                     IAKI"
     gene            complement(3627..4076)
                     /locus_tag="U6309_002601"
     CDS             complement(3627..4076)
                     /locus_tag="U6309_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506023.1"
                     /translation="MSFNNFLKTFNEFLLEQGGTTYRAVDHYLKGKDKPLKSVFFAPY
                     SSAPNFLSRAGHVITAPISFSIITLELVSSSLYLSLKSLNSLVFSDKKAAKIHIIDSV
                     VHFAVSLITAIGVIVSPIINLIDLIGGAISTMRVKSEPAEQMRPSVL"
     gene            4381..4839
                     /locus_tag="U6309_002602"
     CDS             4381..4839
                     /locus_tag="U6309_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5506024.1"
                     /translation="MLHFKWCEFSELTSKQLYDILALRASVFVVEQHCPYLDPDGRDF
                     FSMHLLGLEENSLVAYLRLFLPTEIENNLTFGRVVTAKSARTKGYGRKLIRELLSYCD
                     IHYPGIRIQCSAQNYLKKFYEEFGFKSYGSVYEEDGIPHIAMQRTLIKPQ"
BASE COUNT         1573 a         1022 c          893 g         1721 t
ORIGIN      
        1 tattaaaaca aaaatcaagg gctccataga accctttgtc cacagcccaa acagaataga
       61 aagaaacact tggtttcagc ggttaaacta tccagctaag ccagtttatt ggatatttct
      121 gtttttaggg tttccaggtt attcgcaaat tcacccattt tttcagaaga gtctgttttt
      181 atattttctt tgccagaaaa gaaaactcct acatatccct ttgctgatga aaagtgtaca
      241 accaatgcag gaataactgc caaggttgca ataactccta taaatcttct gacaaaatca
      301 ggatagccat gccaaccacg atgagttgat aaaactgggc tggcctcctc aagaacctgt
      361 ttacacttcc tttcaaattt ttgaaaagat tgaggcgtaa tatcatccat aaaaaaatca
      421 tttcttactg tggataagtc attaattagt gcttgcatga cattggttgc ttcgtgaaga
      481 gtaccatatt ttttattcag taattctgct tttctttgta attcatctaa taatttattg
      541 aatgaatttt gatacggcat aatttgagaa cgcaacatct gttgattatg tcgttgtttt
      601 gcatgtagtt gatcttcttg agtaaaaaat ttttcgaggt gtggacaata ctttaaaaac
      661 aatgcgatta attttaaatc cccattattt atcgcctggg ttaaatgagc cttgttgaat
      721 tggtctttat aagcactgtt ggctttaaaa tggtcttcca tgcgctgata ataagtatca
      781 aataaggcaa ttatctttga tgcctttaca ggatcggttt tatcatagca tctcgcccaa
      841 tcaagtgcgg ttcgtccccc agttggggtt tctccaattt tatatcgatg actcccagac
      901 acttttgttc tgacatgaat atcagcacct gaatccatta aatattccac gatatccaca
      961 tagccattga cacaagccca aagcaacgga gtttgttgga aagagtcttt ggattccaat
     1021 aaatccggtc tatgttctat aatgatttta accagctcaa aatctccttt ttttgctgcc
     1081 tgaattaaat cattcccgtc aatattcaac gcatgacgag agtctgcgtt gagaaacgct
     1141 cgaatcaaat caatatgaac cggtctactc tttatcactg aaataaacac ggatgggaaa
     1201 acaagattgg catagttttc aattaaaagt tttgcatgtt cgtatttctt tttttgaatc
     1261 acttcactta agatttgtga cttttgatat ttcgtcaggt agcgaacttt tgacgccaca
     1321 gcttcagcta tcccttcgtc attcacttct ttatctagca gaacttttat ctgttgaaac
     1381 tctcttgatt tatcaatata agattgtctt tgattataaa aaatttcaaa attttcagat
     1441 gaaattttct tttgctgctc caaacttcgt atatttccct cttcattaat agaactggtg
     1501 ctccaacaaa gacgccctcc catcacgata taattggaat cctggagcaa ttgagcactg
     1561 catgctattc ttagttcatc aaaatgttca gcatcaatat gagccaagac gatgccataa
     1621 gttgattcag caatagacgc gtattctgca gaagtcagct caaattcatc ggccatcatt
     1681 ctcataaacg ctttttcatc gttacataat tgtaatacca aattattcaa cgtccccctg
     1741 tcttcaaaca aacccaggaa tttttttgcc ttttctttgt tttttaaacg caactgcaaa
     1801 gaacacaaat agagaaacgt ttgtaaaata aacagctgat aagtgttcct gcgtttacat
     1861 cctgattcag aatcatcatt agtgctttcg ttatatttat caaaagcctg attgtagaca
     1921 tctgcaaatg ttttttgctt ggttcctgat tgttcactca gcaaatttgc ttgtttaaat
     1981 attgatttaa tggcatcgac tatctcttcc ttttgccagg tactaaagtc ttcgttgtct
     2041 ccaaaggcag cgaccaaatt gccttccatg acttcgaaaa cagctgattg ttgattttct
     2101 aaagaggtat cctgtttttg tagaatttca taaaccgctt taaataaagc ttgaggatct
     2161 ttattatctt ttttgcataa gtaatcgatg gggtctgcat ccagttttcc cagcgcatca
     2221 gcaatcaggt ccaagttacc ctctgcagca ggcttttgca caagagacaa tgcttccagg
     2281 cattgattaa tgtcggactg ggcaagtaat tgcttgtagc gggatacacc ggggtttata
     2341 agactcatta cgtttggcaa atcaaattgt cgcactctgt ctggatacac cgttacaaca
     2401 aactcgctct ttgataccat gatttcatcg ctgtgactaa aatcattttt catgtcttca
     2461 aagcctggat ttccttttct gaaagcgaga gcttgctctt tcaactcatt gagatttgct
     2521 atgattgttt caagaaatag ttgctcatcc acacggattt cagtagacga tttgagacca
     2581 tttaattcat gaatcagact tgagaacaat tcggtaaagg gtttaactga gtgacgacct
     2641 ccggatttct gcttaccata aaaaaaagtg cttctttcca gatctgattt gcacgtatta
     2701 tcgccaaata aataaagaaa aattggcttg gtttcatcgg cagtaaaatt gtcggaaata
     2761 ctggccgcgc ctgttaaagg cacaattatt gatagctcac ctgatttatc taaatgaaca
     2821 aaggggtatt tttcgaatat tttggctaat tggtctttca atttcgttat actctttcat
     2881 tttaagcttt tttggcacca ttgtaatata gtttgcttaa gacaaaatta ataacactgt
     2941 gggcataaat aagcccagaa aaaacctctt gttgtctctg ccaacaagaa aatcccaaga
     3001 tttgaaaaat gaagaacttg cctattattt acaaacactg ccaatttttt taatatccgc
     3061 tgattcaata acaaggagtt tccagtggct tttcaaagat catttttttc tcatatcgaa
     3121 gacgattggc gttattatca aaacattcgt tcaaaatact ctgatgctat acctatacca
     3181 caaagaaaat atttcgaacc cattcattcc atagattcat tcgctacatt agcagtacgg
     3241 cctattgaaa aacctctttg gcttggcgtt cacacggcag gttttttgct gaaagcgata
     3301 atccacttag taggggcatt agttctttca ccatttgctt tgatctttgc ggtatgtgtt
     3361 ccgagatccg aattgagaga gcaaactgtc tcatcattta aatctactgc tgccggatct
     3421 atagttgctg caggtatggc ttgtgttgca ttactctcaa ctttaatgtc gcttattttt
     3481 aatccgttgt atgcattaag tcgaagtgcc gccacaggaa tcgatcatct taattcagta
     3541 accgaatcct gttgcggtct gactatagca aaaatctgat taggtggttc aatcgcaacc
     3601 cttatttatt tcaaatctgg cgaaaactag agaacagatg gtctcatctg ttctgctggc
     3661 tcacttttta ccctcatagt agatattgct cctccaatca aatcaatcaa attaataatg
     3721 gggctaacaa tcacaccaat tgcggtaata agagaaacag cgaaatgcac cacgctatca
     3781 ataatatgaa tctttgcagc ttttttatca gaaaaaacca ggctgttgag agactttaag
     3841 ctcaaataga gagacgatga aacaagttct aaggttataa tagaaaaact aatcggtgcc
     3901 gttatcacat ggcctgctct ggataaaaaa ttaggggcag atgaataagg agcaaaaaaa
     3961 actgatttga ggggtttgtc tttccctttc aaataatggt caacagcccg atatgtggta
     4021 ccgccttgtt ctaataaaaa ttcattgaat gtttttaaga aattattaaa cgacatcgat
     4081 agatactccc tgttaaaaaa atcctcacca taatatatga atttgcatta attactaaag
     4141 ataatttcga tgatgatttt tacttcaatc cattgcctgt aaatttgccc tggtataacc
     4201 agaacttgcc aaatacatcc agttggaagc aaaaaatgtt tttaataagg tagttaattt
     4261 taactaattg acttaattaa aaacattttt taacaaaatc ctgatgatta ctccacttga
     4321 tttcagagtc ataacttcag cataatgcat cgaagatttt agccggattg ggagtatttc
     4381 atgcttcatt ttaaatggtg tgagttttca gaattaacaa gcaaacaact ctatgacatt
     4441 ctggcattac gagcgagcgt ttttgtagta gagcagcact gtccctacct tgatccagac
     4501 ggtagagatt ttttttccat gcatttattg ggcctggaag aaaattctct cgtggcttat
     4561 ttgcgcctgt ttcttccaac tgagatcgaa aacaatctca catttggccg tgttgttaca
     4621 gctaaatcag cgcggactaa aggatatgga agaaaactga tacgtgaatt attatcttat
     4681 tgcgacatac attaccctgg tattcgcatt caatgctccg cccaaaatta cttgaaaaaa
     4741 ttctatgaag aattcggctt taaaagttat ggctcggttt atgaggagga tggtattccc
     4801 catattgcca tgcaaagaac cttaataaaa ccgcaataac caatccgggt ttcctggaag
     4861 taatctatca gcccgatcaa attttaaatt gacatcagtg ccacaataaa gcacactttt
     4921 tatcatgttt actcattaat aatattattt aacgcgagct atggataaaa tcagtcatta
     4981 tctcgcagcc tagtatcgga tttcatgttt catgccaggg cttcgagacg atgcttatgc
     5041 atctcctcag cccgaacgtt ccctgggtat cgactggttg ttatgtcatc tttatccata
     5101 acttacgtta tttaaagtat tattctttgg agatgttatg acgaaaattg tttatttaca
     5161 aaacgacccg gataagatgc tctattataa agacagcgtc cccaaagat
//
LOCUS       DASLOE010000186         5126 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.187, whole genome shotgun sequence.
ACCESSION   DASLOE010000186 DASLOE010000000
VERSION     DASLOE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.187"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            161..2806
                     /locus_tag="U6309_002603"
     CDS             161..2806
                     /locus_tag="U6309_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506025.1"
                     /translation="MDVGQKIRILKRYLKEKSVTPEDVNLNDQDLTAYAGLDKLLDYI
                     SIHNNGLFDFTTLESDEKQWKQYVDILNYSATYSPGSPPALIDTSERKAQFGLQYWLN
                     TRTIEALPRDNYYTRTVTLSDEADEEFDQLIRESRAKLYKIAQTFPEVSSFNPTNIIQ
                     VTNQISSIKEKLNTLAGNNPQITLLQKKLNVLTDLNKINQLERLNIPQDNGSNLSYCT
                     LAETIEGVTEKIPLITLTKVKEQLRHLCAQNELDLDSYSESREQYYKLGIEGILKLPK
                     HGDIKEVQREAIALNISRLMGLDTTSSTTVSYNGHPALFIPFDNINLLNEFSSGKIFK
                     VGMGLSGQSYTHYSTIKPLGEGIQADLYIKDFGHALALLYLCCDTDAIGGYCKNKALR
                     NYSSLYIFDQVITDTDKFILDSRISLQPDQFIMKHTRHGQGRNRTLIEDSSLVTKYAS
                     LMRLKELKRIITQYCDHISWLHHNRTQVIKKQLQEELTKDERARLTEELNDVEILEKD
                     AEQIKNQILIRINKITEVLPQTTETVGSDVIRQCLILEKLLHNPVLYSDDGRPYKNPW
                     TTRQANTVQTIHDLQNGKIQITFKFNIPEEMVDFIKRHGGGDSLTISSPKVITISKAH
                     LDGLRENMLHPEHSPELSLNTDYLDDKDLCIIKMAYRIGNRTKILNAVAEYRVLMNNE
                     KVSFHNKIECLAQTEAKIKALIELANDKGLGMHILKKFYFDAQQRLQKLINPEVLPHD
                     IGQAFAAALRLDRVSEFNKVVREAIVQNKVTDPQFVIFLTNCIKMESLAKNYKDAQKA
                     SRALLIESQRIINHLKSPVVPLIVQLGGQVHSQAELDTDGLANLENDLQLDFEKPDTE
                     SMTTMDLHQAVTKEEMIGNGMTVKV"
     gene            2882..3439
                     /locus_tag="U6309_002604"
     CDS             2882..3439
                     /locus_tag="U6309_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEN5506026.1"
                     /translation="MPVHERRQHFRIDDHIYFDYRILAPGEFCSDIEITNQLLGENGQ
                     KYMEAAQYFQGIDYELAELTQVMAVKEPALAHYLNLLNAKIDYLSRQMLMSSKVQLRK
                     VNISLGGMAFKTMEQIKEKTNLKIILYTKPKMIPIIVDAVVVYSQYQSETHYRTAVAF
                     CNLTSEQEQLLSQHILQAQIKSRAD"
     gene            3515..3784
                     /locus_tag="U6309_002605"
     CDS             3515..3784
                     /locus_tag="U6309_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEN5506027.1"
                     /translation="MSRTVFCCKLKQEAEGLEKQPFPGELGKKVFNEVSKQAWNMWLS
                     HQTMLINEYRLNLIEARAREFLKEEMQKYFFGEGSEKPSGYKEIK"
     gene            3796..4722
                     /locus_tag="U6309_002606"
     CDS             3796..4722
                     /locus_tag="U6309_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEN5506028.1"
                     /translation="MFHDLLEFNEEVLDAINDKSPIVALESTIISHGMPYPDNLTTAI
                     EVENIIRRQGAIPATIAMHQGKIRIGLTQEVMEYLALQKEVIKASRRDISFVLSRKVT
                     ASTTVAATMFCAHMAKLPLFVTGGIGGVHQDVTMSFDISADLIELSNTPVTVVCSGAK
                     SILDLPKTLEVLETFGVPVIGYATDEFPAFYSRSSGIPVPQRLNSVEEVANLMSIQQK
                     LNMKNGIVVANPIPVSAELSDEEISPYIKQAHEEAKHMSGKSLTPFLLKRIAELTAGK
                     SLEANIELIKNNAFLGAEIAIAYQKKLFSKKT"
     gene            4773..4848
                     /locus_tag="U6309_002607"
     tRNA            4773..4848
                     /locus_tag="U6309_002607"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4806..4808,aa:Phe,seq:gaa)
BASE COUNT         1799 a          938 c          964 g         1425 t
ORIGIN      
        1 tatagaaaat aatatacaaa aatcagagtc aaaataatga cttatttttg actgggaagt
       61 taagtaaaaa taactaaaaa tttgaaggaa aacattgaat aggaaaagca tttttataaa
      121 catggaaata agtctaattg atactataag gaaatagaca atggacgttg gccaaaagat
      181 aaggatttta aaacgttatt taaaagaaaa aagtgtcacg ccagaggacg tcaatctcaa
      241 tgatcaagat ctaacagcct atgccgggct ggataagcta ttggattata tttctataca
      301 taacaatgga ttgtttgact tcacaacatt ggaatctgat gaaaagcagt ggaaacaata
      361 tgtggatata cttaactatt ccgcaaccta ctcaccaggc tctccccctg ctttaatcga
      421 taccagtgaa cgcaaagctc aatttggttt gcaatattgg ctaaatacga gaacgattga
      481 agcactgcca agagacaact attatactcg tacagtcacc ttatccgatg aagcagatga
      541 agaatttgat cagctgatta gagaaagcag agccaaattg tataagatag cgcaaacttt
      601 tccagaagtc agttctttta atccaacaaa tattatccag gtaaccaacc aaatctcttc
      661 gattaaagaa aaactgaata ctttggcagg caataatcct caaataaccc ttttacaaaa
      721 aaaactcaac gtactaacag atttaaacaa aattaatcaa ctcgagaggc tcaatattcc
      781 ccaggacaat ggttctaact tgagttattg tacgctagca gagactattg aaggagttac
      841 tgagaaaata cctttaataa cacttaccaa agtgaaagag caattgagac atctttgcgc
      901 tcaaaatgag cttgatttgg atagttatag tgagtcaaga gagcaatatt ataaacttgg
      961 tatagaaggt atactcaaat tacccaagca tggcgacata aaagaagtac aacgtgaagc
     1021 catagccctt aacatctcaa gattaatggg tttagacact acttcttcaa caacagtaag
     1081 ctataatggt catcccgcct tgtttattcc ttttgacaac atcaatctat taaatgaatt
     1141 ttcttcaggt aaaattttta aagtaggcat ggggttaagc gggcaaagtt atacccatta
     1201 ctccacaatt aaaccattag gtgaaggaat ccaggccgat ctttatataa aggattttgg
     1261 tcatgctctg gcattacttt atttatgttg tgatacagat gctataggtg gatattgtaa
     1321 aaacaaagca ttaagaaact atagtagcct ctatattttt gatcaggtca ttacggacac
     1381 ggacaaattt atccttgatt cacgaatcag tcttcaaccc gatcagttca ttatgaaaca
     1441 tactcgtcat gggcaaggga gaaacagaac cttaatcgag gattcttccc tagttaccaa
     1501 atacgcaagc ctcatgcgac ttaaagagtt aaaacgcatt attacccaat actgtgatca
     1561 tatttcatgg ctgcatcata atcgtactca agtcattaaa aaacaattgc aagaagaact
     1621 aacaaaagac gaacgcgcac gattaacaga agagttaaat gatgttgaaa ttttggaaaa
     1681 agatgctgag caaataaaaa accaaattct aattcgcatt aataaaataa ccgaagtgct
     1741 acctcaaacg actgagaccg ttggctccga tgtcatccgg caatgtttaa ttctggagaa
     1801 attactacac aacccggttc tctactctga tgatggaaga ccttataaaa atccatggac
     1861 gacacgacaa gcgaatacag tacaaacgat tcatgattta caaaatggaa aaatacaaat
     1921 tacttttaaa ttcaatattc cagaagaaat ggtcgatttt atcaaacgcc atggcggcgg
     1981 agattctctg actattagct ctcctaaagt gataacgatt agcaaagccc atctggatgg
     2041 ccttagggaa aatatgttgc atcctgagca ttcacctgaa ttatctctta atactgatta
     2101 tttagatgat aaagacctct gtattataaa aatggcttat cgtataggta atagaaccaa
     2161 aattttaaat gctgttgcag aataccgcgt ccttatgaat aatgaaaagg taagcttcca
     2221 taacaaaata gaatgtcttg cccaaactga agcgaaaatc aaagcgctga tagaattggc
     2281 aaatgacaaa ggcttgggga tgcatattct taaaaaattc tattttgatg cgcaacaacg
     2341 actccaaaaa ttaattaatc cagaagtgtt gccccatgac attggtcaag cctttgccgc
     2401 agctcttaga ctggacagag tatcagaatt caacaaggtt gtgagagaag ctattgtcca
     2461 aaataaagtg actgaccctc aatttgtaat ttttttgaca aactgcatca aaatggaatc
     2521 tcttgccaaa aattataaag acgcacaaaa agcaagcaga gccttattaa tcgagtctca
     2581 gcgaattata aatcatctaa aatcgcctgt agttccttta attgttcaat taggagggca
     2641 agtgcattca caggctgaat tagatacgga tgggttagcc aatcttgaaa atgacctaca
     2701 gcttgatttt gagaaaccag atacagaatc aatgaccact atggatttac atcaggctgt
     2761 tacgaaagaa gaaatgattg gaaatggaat gacagtaaaa gtttaataga tttcttttaa
     2821 aactatctgt aatgcagcaa atgaaaagag agatgtttaa acttggaata atggagtgac
     2881 tatgccagta catgaaaggc gtcaacattt tcgtattgat gatcatatct attttgatta
     2941 tcgaattctt gcaccgggtg aattttgttc cgatatagaa atcactaatc agttactagg
     3001 tgaaaatggg caaaagtaca tggaagcagc gcaatatttt cagggaattg attatgagct
     3061 tgccgagcta actcaggtaa tggcggtgaa agaacctgct ttggcgcatt accttaatct
     3121 tttgaatgcc aaaattgatt atttatctcg acaaatgtta atgtcgagta aagttcaact
     3181 acgtaaagtc aacatcagcc tgggcggcat ggcatttaaa acaatggagc agattaaaga
     3241 aaaaaccaat ctaaaaatca ttctgtacac aaaacccaaa atgataccca ttattgttga
     3301 tgctgtagtg gtttatagtc aatatcaaag tgagacgcat taccgaacag cggtggcttt
     3361 ctgtaatttg acaagtgagc aagagcagtt gctttcacaa catattttac aagcccagat
     3421 aaaaagccgt gctgactaag atggagatgc tgtagattgg aactgttttt tctaggataa
     3481 tgagccttca aattaaaata acgaggttat atccatgagc agaacagtat tttgctgcaa
     3541 attaaagcaa gaagcagaag gcttggaaaa acaacccttc cctggtgaat tgggtaagaa
     3601 agtttttaat gaagtatcaa agcaagcatg gaatatgtgg ctttcccatc aaaccatgtt
     3661 aatcaacgaa taccgcctga atttgattga agccagggct cgtgagtttt tgaaagaaga
     3721 aatgcaaaaa tatttttttg gtgaaggttc agaaaaaccc tctggctata aagaaataaa
     3781 ataaaggatt aatttatgtt tcatgacttg ttggaattta acgaagaagt actggatgct
     3841 atcaatgata aaagcccaat tgttgctctt gagtcgacca ttatctctca tggaatgccc
     3901 taccctgata atttaactac ggcaatagaa gttgaaaata ttattcgtag acaaggcgcc
     3961 attccagcta ctatagcgat gcaccaaggg aaaatccgta ttggtttaac tcaagaagtt
     4021 atggagtatc tcgcgctgca aaaagaagta ataaaagcct ctcgcagaga catttctttt
     4081 gtattatctc gtaaagtgac tgccagcacg acggtagcag caaccatgtt ttgtgcccat
     4141 atggctaagc tccccctatt tgtcaccgga ggtataggtg gtgttcatca agacgtgacg
     4201 atgagttttg atatttcagc tgatcttata gaactatcca atacgccagt taccgtagtc
     4261 tgttctggcg caaaatcgat tctcgactta ccaaaaacat tggaagttct tgaaacgttt
     4321 ggggtgccgg tcataggtta tgccacagac gaattccctg ctttttacag cagatcgagt
     4381 ggaataccag tcccacaacg cttaaactcg gtcgaggaag tagcaaatct catgtcaatt
     4441 caacagaaat tgaacatgaa aaacggaata gtcgtcgcaa accccattcc agtatcagcg
     4501 gaactatctg atgaggaaat cagtccatac ataaaacagg ctcatgaaga agccaaacac
     4561 atgagcggaa aatcattaac ccccttcctg ttaaaacgta ttgctgaatt aactgcaggc
     4621 aagagcctgg aagctaatat tgagctcatc aaaaacaacg cttttctagg agcagagatt
     4681 gccatagcct accaaaaaaa attattttca aaaaaaactt aatcaacact tgactcaaag
     4741 tcgatctcgg agtttaatag caagccttga gtggccagat agctcagtcg gtagagcaga
     4801 ggactgaaaa tcctcgtgtc ggcagttcga ttctgcctct ggccaccatt agaatcaatg
     4861 agttacgtaa aaatcaaaag ggcgaatttt aactccaagt gcgacaatca tttttatact
     4921 gctgtataaa cagttcgttg ggcgttttat aaccgataca ttttctaggg caccggttca
     4981 atttagctgc tattaagtcc aactcctctt gggttattaa gttaatttta gcatttttag
     5041 gtaagtattt tcttattcga ccattcatat tttcattact tcctttttgc catggggagt
     5101 gaacatcaca ataataaact tcacat
//
LOCUS       DASLOE010000187         5068 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.188, whole genome shotgun sequence.
ACCESSION   DASLOE010000187 DASLOE010000000
VERSION     DASLOE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.188"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            95..2044
                     /gene="flgK"
                     /locus_tag="U6309_002608"
     CDS             95..2044
                     /gene="flgK"
                     /locus_tag="U6309_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEN5506029.1"
                     /translation="MSILNIAYSGLNAFQRALDVTGNNIANFKTRGYSRQSIQFTPIA
                     SNRYAGSYIGAGVSVSSIYRNVDQFANAQVRSTLSYRTQYDAFYNQAIQIDKLLSQDG
                     SSISVPLQTFFDSIGQLNSTPDNIATRGVVLKQSQLLAQQFNSLQIKLEEYERNSALQ
                     VTESVKIINRITKELAEVNGKLLGNNNIPELLDHRDELLKQLSGYTDLSIFDQGDGTI
                     SVGIASGDMLVAGTQQRDLVVGTGKDSIFGTRIFLSSGGNNQVDITDRLTTGMLGGLI
                     DYEKNVLGQASQLIGQMAIGLAQTFNAQHKLGMDMNSQIGKDFFTDFNSPGQMLKRST
                     ASADNSGTAVLSVNISDISQVKLSDYDLIISDTGANELRLIRKSDGTSTTLTWSSSPP
                     APPAGQVVIDGMTITVNDLSQLANNDHYTLTPTRGAARDLALEIKDAYEIALASPVKT
                     TASLNNTGQGQIILGPVLNTASVNKQFRIDFISDTQYNLVNVTDSTTAGPFAFVPNTN
                     NVIQIPDGITPSYSVVLSGIPKSGDQFTTDYNTGGFGDNRNGLILGNIQQNKIFSNGS
                     ETLFDRYGSLLAEVGGRTNQAKTSFESADILHKQALDFQDSKSGVNLDEEGANLLVFQ
                     QAYQAAGKLMEISNQIMNLLFDIMR"
     gene            2048..3283
                     /gene="flgL"
                     /locus_tag="U6309_002609"
     CDS             2048..3283
                     /gene="flgL"
                     /locus_tag="U6309_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEN5506030.1"
                     /translation="MRISTNQIYQRGLDNLLTQQERLARLQDQQASGIRVRTPSDDPV
                     ASAQIELIKQRISNIELLQNNRQAADSALRLEEGILSNTVNSLHRLREIQIQSGNPSL
                     SEEDRKTLAVEAQALLNQLLDYANTKDSNGSYMFSGSKSLTQPVSLNLSGQYVYNGDN
                     TQRFQAVTTSLLVAVNDTGDNVFMRIPSGNGRFAIRETVTPNTGTASVSSGSVINEAA
                     FVPDNYTMAFALNSQGNLVVMVSGTLSGNVIPPSGLPDDAPLYQEGSAISFNGMEMVV
                     SGLPKAGDSFSISPAKNESIFSTVQRMINNLNKPYVSSVEKAATQTENNQLLAQIDSA
                     LGHILSVQSDLGARLNQLETAEKANNDYLDISAATLKKLREIDPVAVATELNLQLVNL
                     QAAQLSFTRIQGLSLFNFL"
     gene            3478..>5068
                     /locus_tag="U6309_002610"
     CDS             3478..>5068
                     /locus_tag="U6309_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN5506031.1"
                     /translation="MKTVNSQNGTSAEPSVNLGKLSTAKNLVFRGGGSKGVAYVGALQ
                     SLSEQGVLENIENVAGSSAGAMTAAIVACGGSAELMDYFCSANSLADFVDNRLTKKLL
                     PKSEKSPQADSSVDYSSISHEQKGSQLDTESSLAEAKLVTKPSKLTFAIASKGAKLVE
                     AMEHVMRMAIFTHFPESLDNELKNVHEKLNAIEDKGSAEYINLAKRQQILEDTQKIIN
                     GLHDPNFDLTFGHLHTLHQYDPQRFKELYVTGTDENGELRIFSHEKDADMPVSIAVRI
                     SASLPGVFDPVIYEGRKYIDGGAANNLPVNIFFNKEEAYTPGVVTTIGVSCENKLDIT
                     EKEFKNPSFMKRIMGYLGDFVKKMLCKLDVEGAVKEQKREMLKYIDNGNLFVMVAPPI
                     SITTAQMHIDKEEKEGVIKVGKAFCDMQLKSMIDSDYKPQPFDEKTAKILASSGEQQE
                     QSIKKTIDIKSRLQNIKQADRPWSQHVEKTIDRLMLVKGEATQDQIDLCLQWQEIKSV
                     HSNPADRLREFAYAFLASPLAE"
BASE COUNT         1682 a          871 c         1069 g         1446 t
ORIGIN      
        1 ttgaaccagg caatacaacg atgtgaatta tccgaacaaa gttgatagtt aggtccaagg
       61 taaagaaaaa attagttcag ggagtgaaca aaatatgtct atacttaata ttgcctattc
      121 aggattgaat gcttttcagc gtgccttaga tgttactggc aataatattg ctaattttaa
      181 aaccagaggg tatagcagac aatctatcca atttacacct attgcctcaa atcgctatgc
      241 tggttcatac ataggagcag gagtatctgt ttcatcgatt tatcgaaacg ttgatcaatt
      301 tgccaatgct caggttagaa gtaccttgag ttatagaact cagtatgatg cgttttataa
      361 tcaggcaatt cagatagata aattgctttc acaggatgga tcaagtattt cggttccttt
      421 gcaaactttt tttgattcaa taggccagtt gaacagtaca cccgataata tagcgacaag
      481 aggtgtagta ttgaaacaga gtcagttatt agctcagcaa tttaattcgc tgcaaataaa
      541 acttgaagag tatgagcgta attcagcatt acaagtaaca gagtcagtaa aaataattaa
      601 tcgaataaca aaagagttgg ctgaggtcaa tggaaaatta ttagggaata acaatatacc
      661 tgaattactt gaccatagag acgagctgct caaacaatta tctggttata ccgatttgag
      721 tatttttgat cagggtgatg gtacgattag tgttggaatt gccagcggag atatgctagt
      781 ggcaggaacc cagcaacgcg atttggtagt gggtacaggt aaagattcta tttttggaac
      841 aaggatcttt ttaagtagtg gaggaaataa tcaagttgat attacagaca ggctgactac
      901 cggtatgtta ggcggtctta tagattatga gaaaaatgtt ttgggacagg ccagccaatt
      961 aataggccaa atggctattg gtttagctca gacatttaat gcccaacata agttgggaat
     1021 ggatatgaat agccaaattg gcaaggattt tttcactgat tttaattccc ccggacaaat
     1081 gctgaaacga tctacagctt cagctgataa tagtgggact gctgtcttat ctgtcaatat
     1141 atcagatata tcccaagtga aattaagcga ttacgatctg ataattagcg atactggggc
     1201 taatgaattg cgattgataa gaaaatcaga cggtacttct acaaccttaa cctggtcaag
     1261 ctcgccacca gccccaccag cagggcaagt agttatagat ggaatgacga ttacagtaaa
     1321 cgatttatcg caattggcaa ataatgacca ttatactcta acccctacca gaggagcagc
     1381 cagagatttg gccttggaaa ttaaggatgc ttatgaaatt gcgctggctt ctccagtgaa
     1441 aacaacggcg tcattaaata ataccgggca aggtcaaatt attttaggac cagtattgaa
     1501 tacggcttct gttaacaaac aattcaggat agatttcatt tctgatactc aatacaacct
     1561 ggttaatgta acggacagta caacagccgg tccgtttgcc tttgtaccaa atacaaacaa
     1621 tgtcattcaa attcccgatg gaataactcc ttcttattca gttgttttat ctggaattcc
     1681 caaatccgga gaccaattta ctactgatta caatactggt ggatttggtg ataatcgcaa
     1741 tggcttgatc cttggcaaca tacaacaaaa caagattttt tcaaatggaa gcgaaacctt
     1801 atttgacaga tatggtagtt tgcttgctga agtgggaggc agaaccaatc aagctaaaac
     1861 cagttttgaa tcagccgata tactccataa gcaggcttta gatttccagg atagcaaaag
     1921 tggagttaat ctggatgagg aaggagccaa tttgcttgtc tttcaacaag cttaccaggc
     1981 agcgggaaaa ttgatggaaa tatccaatca aattatgaac ttgttgtttg atatcatgag
     2041 gtgatgaatg cgtatttcaa ctaatcagat atatcagcgg ggtttggata atttgttgac
     2101 tcagcaagag agattggcaa ggctccagga tcaacaagct agcggcataa gagttcgtac
     2161 tccatctgat gatccagtgg catcggcaca aattgagctg attaaacagc gcatcagcaa
     2221 tattgagcta ttacagaata atcggcaagc ggcagacagt gccttacgtt tagaggaagg
     2281 gatattatcc aatacagtca attcacttca ccgcttacgt gaaatacaaa ttcagtctgg
     2341 aaacccaagt ctttccgaag aggatagaaa aaccctcgct gttgaagctc aagctttatt
     2401 aaatcaatta cttgattatg ctaacactaa agatagtaat ggaagttata tgttcagtgg
     2461 gagtaaatca ctaacacagc ccgtgtcatt gaatctctca gggcaatatg tttataacgg
     2521 agataataca cagcgttttc aagcagttac aacgagttta ctggtagcag ttaatgatac
     2581 tggtgacaac gtgtttatgc gcattccaag tggaaatggg cgttttgcca taagagaaac
     2641 ggtaacccct aatacaggaa cagcttcagt cagttccggt tcggtaatta atgaagcagc
     2701 ttttgtgccg gataattaca caatggcttt tgctttaaat tctcagggta acctggtagt
     2761 catggtgagt ggtactctca gtggtaatgt gataccccca agtggtttac cggatgatgc
     2821 accattatat caggagggta gtgccattag ttttaatggt atggaaatgg tagtgtctgg
     2881 cttacccaaa gcaggggatt cattttcaat tagtccagca aaaaatgaat ctattttctc
     2941 tactgtgcag cgcatgataa ataacttaaa caaaccgtat gtctcgtctg tagaaaaagc
     3001 agctactcaa actgaaaata atcaattgtt agctcagatt gacagtgctt taggtcatat
     3061 tttgagcgta cagtctgatt tgggagcaag actgaatcaa ctggaaactg ctgaaaaagc
     3121 taataatgat tatctggata tcagcgctgc gacattaaaa aaactcagag aaattgatcc
     3181 tgttgctgtt gcaacggaat tgaatttaca actagtcaat ctccaggcag ctcagttaag
     3241 ttttacacga attcaaggat tatcattatt taactttttg taaaagcata tcaaaaatgg
     3301 agttttttta tcttttacat caagtagcat tgaataatct tacgcggctc aatcattatt
     3361 gtaaatcaat caacataact ataagttata acctataaaa agaactcata agcatgtcac
     3421 atcctgttca tattcacttg ttactatgta ttaaataccc aatgagcagg tgaaattatg
     3481 aaaacggtaa atagtcaaaa tggtacatca gcagagccat ctgtaaattt aggaaaattg
     3541 agtactgcaa aaaatctcgt atttcgcgga ggaggctcta aaggggtagc ttatgttgga
     3601 gcattgcaat ccttgtctga acaaggggta ttggaaaaca ttgaaaatgt tgcaggaagc
     3661 tctgctggtg ctatgaccgc ggcgatagtc gcttgcggtg gatcagccga gttaatggac
     3721 tatttttgta gtgcaaatag tttagcggat tttgttgata accgattgac taaaaaattg
     3781 cttcctaaaa gcgagaagag tccacaagct gactcttctg tggattattc ttcaataagt
     3841 catgagcaaa agggtagcca gctagatact gaatccagtc tagcagaagc aaaacttgtt
     3901 acgaaaccat ccaaattaac tttcgctatt gcatctaaag gcgctaaatt agttgaagcg
     3961 atggagcatg tcatgaggat ggctattttc acgcattttc ctgaatctct ggataatgaa
     4021 ttgaaaaatg ttcacgagaa gctcaatgca attgaggata aagggagcgc tgaatatatt
     4081 aatcttgcaa aaagacaaca gattctggaa gacacccaaa aaatcataaa cgggctgcat
     4141 gatccgaatt ttgatttaac ttttggccat ttacatacgt tacatcagta cgatcctcaa
     4201 cgattcaaag aattgtatgt tactggaaca gatgaaaacg gtgagttgcg gattttttct
     4261 catgagaaag atgctgacat gcctgtttct attgcagttc gtatctctgc gtcattgcca
     4321 ggagtttttg atccggtaat ctatgaagga agaaaatata ttgatggtgg tgctgctaat
     4381 aatttaccag tcaatatctt ctttaataag gaggaagcgt atactccggg agtagtgact
     4441 accattggag tttcttgcga gaataaattg gacatcacag aaaaagaatt caaaaatcca
     4501 tcctttatga aaaggattat gggttattta ggagattttg ttaaaaaaat gttatgtaaa
     4561 cttgatgttg aaggagctgt caaggaacag aaaagagaaa tgttgaaata tatagacaat
     4621 ggaaatctat ttgttatggt ggcgcctccc atttccataa cgactgctca aatgcatatt
     4681 gataaggaag aaaaagaggg cgttatcaag gtaggtaaag cgttctgtga tatgcaactc
     4741 aaaagtatga ttgactcaga ttataaacca cagccatttg atgaaaaaac agcaaaaata
     4801 ttggcaagca gtggtgagca acaagagcaa tcgataaaaa agaccattga tattaaatca
     4861 agattacaaa atattaaaca ggcagacaga ccttggtctc agcacgtaga aaaaacgatt
     4921 gatcgactaa tgttggtcaa aggagaggcc actcaagatc aaatcgatct ttgtctgcaa
     4981 tggcaggaaa taaaatcagt acatagcaac cctgctgata gattaagaga gtttgcctat
     5041 gctttccttg cttctccatt ggcagaac
//
LOCUS       DASLOE010000188         5046 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.189, whole genome shotgun sequence.
ACCESSION   DASLOE010000188 DASLOE010000000
VERSION     DASLOE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.189"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(210..1577)
                     /gene="mpl"
                     /locus_tag="U6309_002611"
     CDS             complement(210..1577)
                     /gene="mpl"
                     /locus_tag="U6309_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEN5506032.1"
                     /translation="MMTHLHILGVSGTFMSALALLARDAGYRVTGSDANCYPPISDLL
                     AAKGISWTEGYDDSTLALQSDMVIVGNAIKRGMPVLEAVMESGKPYMSGPQWLAENIL
                     SKYQVIAVAGTHGKTTTTSMIAWILDQAGMNPGFLIGGVSSDFNTSACLGKGRWFVIE
                     ADEYDSAFFDKRPKFMHYRPNIAVLNNLEFDHADIYPDLAAIQQQFHYLLRTIPKSGV
                     AIKPRDDKALNEVLSRGQFSRIETLALSGEAKWVAELIDDSGSQFKILHNGGVVAEVS
                     WPLIGQFNVENGLAAIAATWNAGVNPVVAAKALEQFSPVKRRLEVRSNRHGITVYDDF
                     AHHPTAITKTIDALKRSGRHQRIFAVMEFASYTMRTGVHAGEMAHALAPVDGAYVLEP
                     QDFNLKDFVSSWTCSYKICKNNGEIVKEIIKTVREGDAVLVMSNRGFNGIHQQLIDSI
                     DKLFM"
     gene            complement(1579..2199)
                     /locus_tag="U6309_002612"
     CDS             complement(1579..2199)
                     /locus_tag="U6309_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506033.1"
                     /translation="MEEYKIFCLVQRALKPQENNQKITMNTLKLLFSYYWRLSLLKET
                     PENSPYSVFLLIVMSVLFSIIMLIQWDIAELDFSRDWSLSLMMATSLILSFIFYTYVI
                     LKFQNLASRLVQTSTCLLSAYIIIHVLATPLFLIDTYLSAENLKTPIFLFIGMIYLFI
                     TLGLSIWQFVITAHIYKFALGTTPVKSVLAAFGLVAVNILTISFWR"
     gene            complement(2252..>5046)
                     /locus_tag="U6309_002613"
     CDS             complement(2252..>5046)
                     /locus_tag="U6309_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02099 family protein"
                     /protein_id="HEN5506034.1"
                     /translation="QNTYQIFTKSLIVESLLSEAINWPPAIQNVLQLKPHGILSDVQV
                     LIKENQINYILTRFDQLGWRAKDNIPGVSNLSGVLNWQPMEGRLELDSENSTFDIKGY
                     PTQKLAIFNGAFDWKELNDGLRLSIERLVASQPELTISLEGAFDQLTQNSVGNIRLAG
                     NFSGKNLQKWMPYLPKKHMKAKLFAWLNNDIKRLGQATGKITINGMAKDFPFDNGNGE
                     FSIVSHVVGGDILITPEWRLIKDLEGYIKLKNRNLDIDIIDGDAQGTPLKQMNLRIDD
                     IGKDRETLLIHGIVHGKAQQMLNFVMNSPLKKKLSALKILLIKGMLSLNLRLEIPLYP
                     ENDDNLARGELTFENNIINIKHQMGDFSVRDVTGNLLFNEKGIQDSALTARAFGYPLN
                     IKIQSEKSPQPLTAISIEGECTVESLKKKWHLPIFSVLKGNILAKALLKITDDPNDLD
                     NMKLSSDLIGLAVDLPVPLGKTYKEKTPLQVNLDFNPQKTLRLRVNYNGRLSTDLLMQ
                     KQKGVFKLKSGQIRLGSAQAVNQDHPGLGIAGTLDGFDLQAWKEVINRFSKENVNPSL
                     LSLVNIIDVKMSKLNFLNQQFDNMSLYAKKRPDNDWSVNLKQKKVDADLTYHSATNSL
                     SGFVKYLHLSEIGSSKNNKDTPSHATPGQIPNLNLRVDNFSIGKIQIGNITLKSHSTS
                     EQWKIDYCRIDSPYYQANFEGEWLQKGKFNQTKLQSKMYLKDLAKSLDRWAINPAVDA
                     GKGYIEFHGGWQGSLYDFSLSKLNGSMYLQLKNGRITHLSPETEEKLGLGKLLSILSL
                     QTIPRRLKLDFSDLSNDGYSFDIFKGNFNVNNGIMSTNNSYIDGPVAYASMKGELDLV
                     RRLYDLDLNISPHITASLPVVATIAGGPIAGLAAWIANKIINQSMQKIAGYSYKISGP
                     WDQPIVQQLNIVKKLP"
BASE COUNT         1592 a         1025 c          822 g         1607 t
ORIGIN      
        1 tttgcaacaa atatttcaaa tcgccaattt tatttggatg aagaaaataa atatattttt
       61 ccgtaggtta ttctaaactc aagagatacc ttttactgag atgatctttt tgtgtttggg
      121 ctttgcaaat aataggacat gacttttatt tcacctgctt ctcaatatca ttatatgttc
      181 tcaagacatt tcagaataaa aatgaatcgt tacataaaaa gtttatctat agaatcgatc
      241 aattgttgat gaattccatt aaatcctcta ttactcatta ccaatacagc atcgccctct
      301 cttactgttt tgataatttc tttaactatc tctccattgt ttttgcaaat tttatacgaa
      361 caagtccagg agctaacaaa atcttttaaa ttaaaatcct gaggctccag gacataggca
      421 ccatcaacag gagctaaagc atgagccatt tctccggcat gtacacccgt tctcatagta
      481 tatgaagcaa actccataac ggcaaaaata cgttgatgtc gtccacttct ttttaaagca
      541 tcaatagttt tagtaattgc ggttggatgg tgggcaaaat catcataaac ggttattcca
      601 tgtcgattgg aacgaacttc aagtcttcgt ttcactggag aaaactgttc taaagccttg
      661 gctgcaacca caggatttac tccagcattc caagtagcag caattgcagc caaaccattt
      721 tctacattga attgccctat caacggccaa gatacttcag caaccactcc cccattatgg
      781 agtattttaa attgtgaacc actatcgtca atcagttcgg caacccattt ggcttctcca
      841 gatagtgcca gtgtttcaat acgcgaaaat tggcctcgtg atagaacttc attcagagcc
      901 ttatcatctc gaggtttgat agccacacca cttttgggaa ttgttcttaa taaataatga
      961 aattgttgtt gaatagctgc caagtccgga taaatatcgg catgatcaaa ttctaaatta
     1021 tttaatactg caatatttgg tcgataatgc ataaatttag gtcgtttatc aaaaaaagca
     1081 ctatcgtact catctgcctc tatgacaaac catctccctt tgccaaggca ggcactggta
     1141 ttaaaatcac tagagacgcc cccaatgaga aatcccggat tcattccagc ttgatccaaa
     1201 atccaggcaa tcattgaagt ggtagtcgtt ttgccatgag ttccagctac cgcaatcacc
     1261 tgatattttg ataaaatatt ttcggcaagc cattgagggc ctgacatata tggttttccc
     1321 gactccataa ctgcttcaag aactggcatt ccccgcttaa tagcattacc gacaataacc
     1381 atgtcagatt gcaatgctaa agtggaatca tcgtagccct cagtccatga aatacctttg
     1441 gcagcaagca aatcactgat aggtggatag caattggcat cgctgcctgt cactctatat
     1501 cctgcgtcgc gagcgagtaa agccaaggca ctcataaaag ttccacttac tcctagtata
     1561 tgcaaatgtg tcatcatgtc atcgccaaaa agaaatagtt aaaatattaa cagcgactaa
     1621 accaaaagca gctaaaactg attttaccgg tgtagtgcct aaagcaaatt tataaatatg
     1681 ggctgtaatt acaaattgcc aaatagaaag ccccaaagta atgaacaaat atatcatacc
     1741 tataaacaaa aaaatggggg ttttaagatt ctccgcagac aaataagtgt caattaagaa
     1801 caatggtgtc gccaaaacat gtataataat ataggcactc aataaacatg ttgatgtttg
     1861 tacaagcctg gacgcaagat tttgaaactt taaaataaca taggtataga atataaaaga
     1921 caaaatcagg ctcgtcgcca tcattaagct taatgaccaa tcgcgagaaa aatcaagttc
     1981 ggcgatatcc cattgaatca gcattataat agagaacaga acactcatta ctataagaag
     2041 aaaaacggaa taaggagaat tctccggggt ttccttcaat aggctaagac gccagtaata
     2101 agaaaatagt aattttaatg tattcatagt tattttttga ttattttctt gaggcttaag
     2161 cgctctctga acaaggcaga atattttata ttcctccatg ggccctaagt gacttcataa
     2221 ctgtttacta aataatcttg tcttccttta gttaaggcaa tttcttcact atattaagct
     2281 gttgcacaat aggctgatcc caagggccag aaattttata actatatccc gcaatttttt
     2341 gcatgctttg attaataatt ttatttgcaa tccatgcagc aagaccagca atcggacctc
     2401 cggctatcgt agctacaacg ggtaagctgg cagtgatatg cggtgaaatg tttaaatcca
     2461 aatcatataa tcgtcttacc aaatccagtt cccctttcat actagcataa gctactgggc
     2521 catcaatgta actgttattg gtgctcataa tgccattatt gacattaaag ttgcctttaa
     2581 atatatcaaa actgtagcca tcatttgaga gatcactaaa atcaagtttt aaccgtcttg
     2641 gaatagtttg caaactcaat atgcttaata atttccctaa accaagtttc tcttcagtct
     2701 ccgggctcaa atgagtaatg cggccatttt tcaactgtaa atacatggaa ccattcaact
     2761 tggacagaga aaaatcatat aaggaacctt gccaaccccc atgaaattct atatatccct
     2821 tccctgcatc caccgctgga ttaatcgccc aacgatcaag actcttggcc aaatctttca
     2881 aatacatttt agactgtagc tttgtttgat taaacttccc tttctgaagc cattcccctt
     2941 caaaattagc ttgataataa ggtgagtcta tcctgcaata atcaatcttc cactgttctg
     3001 atgtagaatg acttttaagg gtgatattcc caatttgaat ttttccaatt gaaaaattat
     3061 caactcttaa attcaaattg gggatttgtc caggagtagc atgtgaagga gtatctttat
     3121 tattttttga agatccgatt tcagataaat gtaagtattt gacaaagcca ctcagagaat
     3181 tggttgccga atggtaggtt aaatccgcat cgactttttt ctgttttaaa ttgactgacc
     3241 aatcattatc agggcgtttt ttcgcataca aagacatgtt atcaaactgc tgattcaaaa
     3301 agtttagttt actcattttt acatcaataa tattaaccaa actcaagagt gaggggttca
     3361 cattctcttt agagaatctg tttattacct ctttccatgc ctgcaaatca aagccgtcta
     3421 aagtaccagc tataccgagg ccaggatggt cctggtttac agcttgcgca ctacctaaac
     3481 gtatttgacc tgattttaac ttaaacacgc ctttttgctt ttgcatcaat agatccgtac
     3541 ttagtcgtcc attgtaatta actcttaaac gaagagtctt ttgtggatta aagtccagat
     3601 taacctgtaa gggggttttt tctttatatg tttttcctaa aggtactgga agatcaactg
     3661 ccaatccaat caaatcggaa ctcaacttca tattatccaa atcattagga tcgtcagtaa
     3721 tttttaataa tgccttagct aaaatattcc ctttgagtac agaaaaaata ggcaagtgcc
     3781 attttttctt tagggattct acagtgcatt caccttcgat tgatattgcg gttaatggtt
     3841 gtggagattt ttccgattga attttaatat tcagggggta gccgaatgcc ctggcagtta
     3901 aggcactatc ctggatgcct ttttcattaa acagtaaatt tccagtaaca tcccttacag
     3961 aaaaatctcc catctgatgc ttgatgttga ttatattatt ttcaaaggtt aattcacctc
     4021 gggccagatt atcatcattc tctggataaa gaggtatttc caaacgcaaa ttgagtgata
     4081 gcatcccctt tattaataat atttttaaag cagataattt tttctttaat ggggaattca
     4141 taacaaaatt gagcatttgt tgtgctttcc catgcacaat tccatgaatt aataaggttt
     4201 ccctatcttt gccaatatca tcaatacgca aattcatttg tttaagcgga gttccttgag
     4261 catcaccatc aataatatca atgtcaagat ttctattttt tagtttaata taaccttcca
     4321 agtctttaat caatcgccat tctggagtaa ttaaaatatc tccaccgaca acatggctta
     4381 ctatagaaaa ctccccattg ccattatcaa atggaaaatc cttcgccatt ccattaatag
     4441 ttatcttgcc tgttgcttgc cctaaacgtt tgatgtcatt atttaaccaa gcaaataact
     4501 tggctttcat gtgtttttta ggcaaataag gcatccactt ctgtaaattt ttcccagaaa
     4561 aattacccgc taaacgaata ttgcctacag aattttgggt cagctgatca aaagcgcctt
     4621 ccagactaat ggtcaattcg ggttgacttg cgaccaggcg ttcaatactc aaccgcaacc
     4681 catcatttaa ctctttccaa tcaaaagcac cattgaaaat cgccagtttt tgggtagggt
     4741 aacctttgat atcgaaagtt gaattctcgg aatctaactc aaggcgtcct tccataggct
     4801 gccaatttaa aacgccggat aaattactca ctccaggaat attatccttt gctctccatc
     4861 ctaattgatc aaatcgagtt aaaatataat taatttgatt ttctttgatt aatacctgaa
     4921 catcactcaa tattccatgc ggttttaact gcagtacatt ttgaatagcc ggcggccaat
     4981 taatcgcttc ggataatagt gattctacaa taagggattt tgtaaagatt tgataggtat
     5041 tctgat
//
LOCUS       DASLOE010000189         4983 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.190, whole genome shotgun sequence.
ACCESSION   DASLOE010000189 DASLOE010000000
VERSION     DASLOE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.190"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            30..1961
                     /locus_tag="U6309_002614"
                     /pseudo
     CDS             30..1961
                     /locus_tag="U6309_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1923..2438)
                     /gene="gstA"
                     /locus_tag="U6309_002615"
     CDS             complement(1923..2438)
                     /gene="gstA"
                     /locus_tag="U6309_002615"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEN5506035.1"
                     /translation="MKLYYTPGACSMAPHIALREAGLKFDLEKVDLMTKKTESGADFT
                     AINPKGYVPALQLDNGQVLTEVGVVLQYIADQKPGSGLAPAAGTLERYRLMEMLNFIS
                     TELHKNFGPLFNPAAGDDAKKAAIEMIGKRLGYLDQQLAGKQYLLGDTFTVADCYLAE
                     HQAFPIGNLFR"
     gene            complement(2751..3764)
                     /locus_tag="U6309_002616"
                     /pseudo
     CDS             complement(2751..3764)
                     /locus_tag="U6309_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3810..4601)
                     /locus_tag="U6309_002617"
     CDS             complement(3810..4601)
                     /locus_tag="U6309_002617"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012955.1,HMM:NF027449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEN5506036.1"
                     /translation="MLVERIWTGNSYRNFNYLIACPETGEALAIDPLDHEKTLNTARV
                     KGWQITQILNTHEHHDHTGGNAAVVAATGAKVIAHHKAAGKIEGMDRGVKAGDVIKVG
                     RHIELECLDTPGHTMCHICLRSHTDEPALFSGDTLFNAGAGNVHYGGSVDELYTTFVQ
                     QLAKLPENTRVYPGHDYIENNLRFTLAREPGNAAAQALLPRVTGHDPATSVVTTLAEE
                     KRFNTFFRLASPEVIAKLRESFPELPEQPDAKTVFTKLRELRNQW"
     gene            complement(4800..4940)
                     /locus_tag="U6309_002618"
                     /pseudo
     CDS             complement(4800..4940)
                     /locus_tag="U6309_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
BASE COUNT         1219 a         1265 c         1354 g         1145 t
ORIGIN      
        1 tttaagatta aattttaacg aggtgcatca tgaaaaccaa actaaacaaa tcaatccttt
       61 ttaaacaagg aaatgctggt gagctttttg aatcttttat tcaaaaagtt gatgaaaatg
      121 ccaagcctaa tcagcttgaa gaaattatcg ctgttgcaaa accccaagtc atagcccctg
      181 taacaaaaaa tacatatacc aaacaatttg aaattatagt atccacttta ctaaccaata
      241 aatccaatat tgtctttaca gaaaagaatg gcaaatattt tgtaaatata aaagggacag
      301 aaacaaatct ggatgacatt ctggctaaca tgcaacgctc tcaaataaat ctgtctactg
      361 aacaacttaa tcagtatgtt aattacgttg atacagaagc aacaactaaa attgaactta
      421 cgaatggaaa aatgagttgc aaggatctaa aaaagaaagc aaccaaatta caacatcttc
      481 atgaaggtga aattgctgga ttaaatatat atacccaaca gtattatcaa tttatgaatg
      541 gtttattaag aggccaacat ccatacaaag gaagcgatgc gaactttcag gaaaatatgc
      601 gtcaggtaat acttcatagc gcagcagcac tctcaggtat tagcaaagga caaaaccata
      661 aacttccatt gaccttccga tacgacggaa atagccttcc caaggatatt attgaaaaaa
      721 gaattcaatg tgcccagtca aaaggtatag aactagaagt ttatgaagac accgcttttg
      781 tcagtagtgc ttttgaaaaa cccgtctcta cttttaatgg acccattcga accgtatttt
      841 atggtatgca aggattggat ataggagaaa tctctcgtta tcccgctgag cgtgaattct
      901 taatcgctcc tggtaaagtc aaatacctgc atcatcgaga agaaaatgga aaacactatt
      961 tcctggcgtg tccagtcact cctcctgcaa gcataaagca aatattaaat ttgccaaacg
     1021 gtggtactta taaacaagct gaaaacataa cactagaagc agagaaggat atggtaaaaa
     1081 aattcgcaca atttgctctc gatgttacga atcaatatct tgagggtaaa gcagaaaaag
     1141 ataaaagcaa atggtcaatt ttagcaaata ttgataatac ctatggaaaa ggctcttata
     1201 aaattcagtt tgcagaagaa ataaaaaaat ccatgaatgg attgcttgcc aatttaaaaa
     1261 atggatatgt tgaacataaa gctgctcact ccagaattat tgaaatactt catcaggcaa
     1321 tcgaaaaagc ccaaaccata gataacatat ccttatctta tgctaaagga ttagatagtg
     1381 ctggtgtgct atctaaaacc ttacaaaaca tctgttttca ggttgaggat gaaatgaagg
     1441 gattagaaac gttcaaaagt tcaataactg agtattttgc agatcatcaa agcttttttg
     1501 aaaacccaag gaatattgag gtaattaata agttgcatca agatcatcta tcaaaaccat
     1561 acgacccatc agaaaaaaac aaagacccta atagtggcga ttggacgtta aattgtggta
     1621 ataataagat tcttcataga ccaaatcatg ggctgtctca cactttaaga ggtgctgctc
     1681 tagttccaga agtactttat tcctacgcga cgcattccac cgataagaag accagagata
     1741 tggttcaaaa cataaacaag gatgacatca aacgaatgca agtcgccatg ttattttctg
     1801 ttgtcggcag aaaaagcgaa ctgggatttg cagataaccc tggggtatat gggggatata
     1861 gaaaagccag cgcagaactt tttgaacaat atgcaaaaac gcactataaa gacttattta
     1921 cctcaccgga acaaattgcc tattggaaac gcttggtgct cggcgaggta gcagtcggcc
     1981 accgtgaagg tgtcaccgag cagatactgc ttgccggcca gctgctggtc gaggtagcca
     2041 aggcgcttgc cgatcatctc gatggctgct ttcttggcgt cgtcgccggc cgccgggttg
     2101 aacagcggac cgaagttctt gtgcagctcg gtggagatga aattcagcat ctccatcagg
     2161 cggtagcgct cgagcgtgcc ggcggccggg gccagtccgc tgcccggctt ctggtcggcg
     2221 atgtattgca gcaccacgcc gacctcggtc agcacctggc cgttgtcgag ctgcagcgcc
     2281 ggcacgtagc ctttgggatt gatggcggtg aagtcggcgc ccgattcggt tttcttggtc
     2341 atcaggtcga ccttttccag gtcgaatttc aggccggctt cgcgcagggc gatgtgcggg
     2401 gccatggaac aggcgccggg ggtgtaatac agcttcatgg aacgctcctt gctcttgaat
     2461 gtgccaacga ggaaaacacc gaaaaaaccc atccctggga tttttcggcc cgccgcagcg
     2521 cgggtcgggg ggataggaaa tccaaccgca tgagcacaag tatagcacac gtttggggcg
     2581 tcaagtatag cacacgtttg gggcgtctca ggcatgcaca caggggactg ctgggttgat
     2641 ttctcaaagc cagttgcccg agatttttcg gtatgacccg tttttggcgg tgacaggcgc
     2701 agcgctgcct cgcaacgttg gcaaaaccac tgttaaccgc tgttgccacc ctaccaactg
     2761 acgcgctgat cgacctcgaa gtccggggcc ggttgggtcg cttcatccca gtctggggct
     2821 ggctccacgc cctctgcggc ttgcgcgtcg cactcgtccc acagtggcgg cccgcgtgcc
     2881 ggggtgatgt gcggcggctc cgtctccacc ccgatgtgct ccagtatttg gcggatatcg
     2941 gcactgtgtg tgatgaaggc gatgatgtgc atttgcccgc cgcagatggg gcacagcagc
     3001 gggaacacct cgtagatacg ggccatcagc accgcccaca aatagtgcgc cgggcgcttg
     3061 ggtggcaccg gctgggcggg ctcggtctgg gttggcagtg ggcttcttac cccaagtgcg
     3121 ccctcgcctg cagccgtgct ggctggctcg gcttgcacct gcgccggttt caccgctgca
     3181 tcacgcgcca gcgccgtgac ggcaggcctc aagggtgagt tcggtgccag cacgccaaag
     3241 tagcggtggc ggtgcgtgcg cggcgggggc accagcttgg cgatacgttc aatcagctcc
     3301 agcggggtga gggtgatctc atcggccttt acgccgcgct tgttgtgagg caggctgccg
     3361 ggctcgctgt gctgcttggc gcagcggtag accagctcgc tgcccgcttt gcgcagccgc
     3421 tccatggcaa acggcgggcg ggcgcagtag cgcagcagcc gctccagccc ggcgcggtcg
     3481 tgcgccgcta tgcacacgct ggtgtccacc gaaaacccgc tgtgcttgta gcccagcatc
     3541 tcttgggcct cgaaactctc cagcaggccc cgtccgacaa aggcgcgcag gatgcgtttg
     3601 cgtaagctgg cctgcacctg acccacggca tccgcagtca cgccggtggc ggggtggaag
     3661 atgatgcgcg atggtgagct ttgagaagct gcatcagcag catcgccctc ctcgcccgcc
     3721 acttcctcaa acacgccatc gactgcgcag acatggaagt gcacaccgtg cggttcacgc
     3781 tgaagtaagg ggccggcgcc ggccgctgct caccactggt tgcgcagctc gcgcagcttg
     3841 gtgaacacgg tcttcgcgtc gggctgttcc ggcagctcgg gaaagctctc gcgcagcttg
     3901 gcgatcacct cggggctggc caggcggaag aaggtgttga agcgcttctc ctcggccagc
     3961 gtggtgacca ccgaagtggc cgggtcatgc ccggtcaccc gcggcagcaa tgcctgcgcg
     4021 gccgcgttgc cgggctcgcg cgcgagcgtg aagcgcaggt tgttctcgat gtagtcgtgc
     4081 cccgggtaga cgcgggtgtt ctccggcagc ttcgcgagct gctgcacgaa ggtggtgtag
     4141 agctcgtcca cgctgccgcc gtagtgcacg ttgcccgcgc ccgcgttgaa cagcgtgtcg
     4201 ccggagaaca gcgccggctc gtcggtgtgc gagcgcaggc agatgtggca catcgtgtgg
     4261 ccgggcgtgt ccaggcattc cagttcgatg tgccggccga ccttgatcac gtccccggcc
     4321 ttcacgccgc ggtccatgcc ttcgatcttg cccgcggcct tgtggtgcgc gatcaccttg
     4381 gcgccggtgg ccgccaccac ggccgcgttg ccgccggtgt ggtcgtggtg ctcgtgcgtg
     4441 ttgaggatct gcgtgatctg ccagcccttg accctggccg tgttcagggt tttttcatgg
     4501 tccagcggat cgatcgccag cgcttcgccg gtctcggggc aggcgatcag gtagttgaag
     4561 ttgcggtacg aattaccggt ccagatgcgt tcgacgagca tgggggctcc tggggaatcg
     4621 atggggggga tcgtgggggt cgggccattg taggaacccg cgcccgcctt cgtgggcgca
     4681 gcgccgcgct actctgccag cgcctcgccg tcctccgcgc gccgtgccgg ggataggaaa
     4741 tccaaccgca tgagcacaag tatagcacac gtttggggcg tcaagtatag cacacgtttg
     4801 taggcttcca gtgccgctgg tgagttggca aatgtgctga atagatttaa ttcagtgccc
     4861 atagcttttt ttgtgccttc gagtagctct ttagctttgc cttgtgcgtt agcgatagag
     4921 atttttttaa tacgttgcac gatacctctc cttatttttg gatagattgg aatacgacag
     4981 tat
//
LOCUS       DASLOE010000190         4970 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.191, whole genome shotgun sequence.
ACCESSION   DASLOE010000190 DASLOE010000000
VERSION     DASLOE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.191"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            175..375
                     /locus_tag="U6309_002619"
     CDS             175..375
                     /locus_tag="U6309_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506037.1"
                     /translation="MNRENEVIEIFLMDISKKEKCKLLQDFLLDCKNEMEAQDQNMHP
                     EVHHNLSQAYQLAQNYLRKLQE"
     gene            462..1316
                     /gene="folD"
                     /locus_tag="U6309_002620"
     CDS             462..1316
                     /gene="folD"
                     /locus_tag="U6309_002620"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEN5506038.1"
                     /translation="MPASLIDGREISALRRTELKQRVQYHVEQGQRAPGLAVVLIGND
                     PASVIYVSNKRKACEEVGITSHSFDLPAETTQEKLIELINELNQSDKIDGILIQLPLP
                     KHINERTIIEHIKPEKDVDGFHPYNLGRLAQRNPFLRPCTPLGIMNLLHHYELNVKRK
                     HAVVIGASNIVGRPMSLELLLAGATVTICHKFTQQLQKFVEIADFLIVATGKMDVIAT
                     DWLREHQVVIDVGMHRLPDGSIRGDIDFKKAVEKVAWITPVPGGVGPMTIVTLLENTM
                     MSAARLRE"
     gene            complement(1319..1528)
                     /locus_tag="U6309_002621"
     CDS             complement(1319..1528)
                     /locus_tag="U6309_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506039.1"
                     /translation="MGLHEEFEDQSFSDYNYDEEIDDHIDDLNQKKKIRRMLEDRLER
                     KRLKEEFKDDFDELSGEFDWDELDK"
     gene            1805..2653
                     /locus_tag="U6309_002622"
     CDS             1805..2653
                     /locus_tag="U6309_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEN5506040.1"
                     /translation="MLSYQHGYHAGNFADVIKHITLTRLLAYLTHKDKPLFYLETHSG
                     RGIYDLKDKQSLKTEEYKEGINPVWLVRENLPSLFLEYISVIKQINLNSTLRYYPGSP
                     YFAINQLRSQDRLYLCELHPTEYNFLLKLPHFNKKVYVNHTDGVSKLNALLPPPEKRG
                     LIFIDPSYERKEEYKEIPYAIKNAYSKFSTGLYCVWYPVVNKAWTEQFLRKMREISSK
                     SVRIELHLNPLINEGMTGCGLWIINPPYTFPSEIKIVLETLTTYFNPGSSSYIIESGS
                     KLCHEL"
     gene            2657..3421
                     /gene="gloB"
                     /locus_tag="U6309_002623"
     CDS             2657..3421
                     /gene="gloB"
                     /locus_tag="U6309_002623"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEN5506041.1"
                     /translation="MTILPISAFSDNYIWTFIDKIAGVLDCVDPGEAGPIIRFAQSNQ
                     LTLRTILLTHHHYDHIGGVDLLIKQWPSCKVYGPIDERINNVTHPIKQGQSVQVGSLH
                     FHILFNPGHTSTHISYYEPQQGWLFCGDTLFSAGCGRVFDGTIEELHESLLLFKKLPR
                     NTKVFCAHEYTLQNLKFAHTVEPCNSSVINYMQQILKQPSPCTLPSNIDLELSINPFL
                     RTDKEQVKQYALSHGANSSDSLDVFKVLRNQKNSFK"
     gene            3535..>4970
                     /locus_tag="U6309_002624"
     CDS             3535..>4970
                     /locus_tag="U6309_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN5506042.1"
                     /translation="MNCNLLIIKRFFSFILIFIGISNPAISGTAPDVWDVLRSEFTLS
                     HEVTRPEVQEQIRWLISHPSYINKVSSQSEPYIYHVLGEIKKRKLPGELALLPMIESA
                     YDPFAYSGAGAAGIWQLMPRTGSDLGLKQDWWFDGRRSIGPSTDAALNYLVYLNKYFS
                     GNWILAIAAYDSGEGTISRALKSTARNGRKVQFWNLPVPRETKVYIPRLLALAEIINN
                     PKRYKISLPNIPYLPYFEEVNIGSQIDLNHAAKLAGISYKELIKLNPGYNRWTTAPYK
                     PFKLLIPAEKVERFNLNLSNIPEDKRVNWTKHQVKRGDSLDAIAKRYHTTVNLIKQLN
                     QLTSNKVQLNQSLLIPSSKNSPVVAKIPPQRKKPATKQLTPMKNVRIIHIVQNNDNYQ
                     KIQKAYGVAAREIQNWNKLANNAPLRKGQRLVIWKRVKQPVEYIVKSGDSLSVIAKKH
                     QTKVNTIISLNPGLKKSMPLRLGQKILIG"
BASE COUNT         1712 a          951 c          854 g         1453 t
ORIGIN      
        1 aattactccc taaaaatcat aagatgatgt ataaaattac atcacatatt tcatctaatt
       61 taacaagcta aatcaattgt ggccttttaa aatccaacca gaaaaactca taactccaat
      121 agaccttcaa ctataattat attaaggatt agtaaaatta aatttggaat agcgatgaat
      181 agagaaaatg aagtaatcga aatatttcta atggatatca gcaaaaagga aaaatgcaaa
      241 ttgttacaag attttcttct ggattgcaaa aatgaaatgg aagctcagga tcagaacatg
      301 catcctgaag tgcatcacaa tttatcgcaa gcttatcagc tggcgcaaaa ttatttaaga
      361 aaactgcaag aataagtgta ttttccttgt tgcatatgaa tcatattgca ttagaataac
      421 ccgtaattca taatgtactc cataaaaatt gcgagaagaa aatgccggca tcactaatcg
      481 atggaagaga aatctctgcc cttcgcagaa ctgaactaaa gcaaagagta caataccatg
      541 tggagcaagg gcaaagggcg ccaggacttg ctgttgtttt aattggcaat gatcctgcat
      601 cagtgattta tgtcagtaac aaaagaaaag cttgtgaaga ggtagggatc acctctcatt
      661 ctttcgattt gcctgccgaa acaactcagg aaaaactaat tgaactgatc aatgaattaa
      721 atcaatcgga taaaattgat ggtattttaa tccaattacc tttgcccaaa catattaacg
      781 aaagaactat aatagaacac ataaaacccg agaaagacgt ggatggattt catccttata
      841 atttaggccg acttgctcaa cgaaacccat ttttaagacc atgcactccc ttggggatta
      901 tgaatttatt acatcactat gagctcaatg taaaaagaaa acatgccgta gtgattgggg
      961 catccaatat tgttggaagg ccaatgagtc tggagttact attagctggt gctaccgtaa
     1021 caatatgcca caagtttacc caacaattac agaagtttgt tgagattgct gattttctaa
     1081 tagtagccac tggaaagatg gacgtcattg ccactgactg gctcagagag catcaagtag
     1141 tgattgatgt aggaatgcat cgtttgcccg atggcagtat cagaggtgat attgatttta
     1201 agaaggcagt cgaaaaagtt gcctggatca cacctgttcc agggggagtt ggcccaatga
     1261 ctattgtcac tttgcttgaa aatactatga tgtcagcagc aagattaaga gaatgaagtt
     1321 atttatccag ttcatcccaa tcaaattcac cacttagctc atcaaaatca tctttgaact
     1381 cttcttttag acgcttacgt tcaagcctat cctcaagcat acgcctgatt ttcttttttt
     1441 gatttaaatc atctatatga tcatcaattt cttcgtcata gttgtaatct gaaaatgatt
     1501 gatcctcaaa ctcttcatgt aaacccatct tagtctccca atttactccc ctcaaaaaaa
     1561 gtatagtgca taaattatga aataaagtaa ctggaagtaa aataggcctc ttgcataacc
     1621 tgattatttt gcttcaggac atggcgcata cgttttgaga ggtggagtac ataagtgttg
     1681 aagaaatgct tacgatgcgg aagtaaaata aaagagacca cttatgcaag aagtctaata
     1741 tgcagctata gcataaacca ttattttgat ataatttgga aaatatcttt tgaagccctc
     1801 tttcatgctc agttatcaac atggctatca tgccggaaat tttgctgatg tcatcaagca
     1861 catcacccta actcgactat tggcttatct aacccacaaa gataaaccct tattctatct
     1921 tgaaacccat tcaggtcgag gaatatatga tttaaaagac aagcagtctt taaaaacaga
     1981 agaatataag gaaggaatta atccagtatg gctcgttaga gagaatctcc cctctctttt
     2041 tcttgaatat attagtgtaa ttaagcaaat aaatcttaat tcaacattgc gttattatcc
     2101 tggctccccc tatttcgcaa tcaatcaact gcgctctcaa gaccgattat acttatgtga
     2161 attacaccca acagaatata atttcttatt aaaacttcct cattttaata aaaaagttta
     2221 tgttaaccac acggatggag tttcaaaatt aaatgccctt ttgccgccac cagaaaaacg
     2281 tgggttaatt tttattgatc cctcatatga aagaaaagaa gaatacaaag aaattccata
     2341 cgctataaaa aatgcttact caaaattttc taccggctta tattgtgttt ggtatcctgt
     2401 tgtcaataaa gcatggactg aacaattctt gaggaaaatg agagaaatta gttccaaatc
     2461 ggtaaggatt gagcttcact tgaatccact cattaatgaa ggaatgactg gttgtggtct
     2521 ttggataatt aaccctccct atactttccc ttcagaaatc aagatagttt tagagacatt
     2581 aacaacttat tttaaccctg gctcatcttc ttatatcatt gaatcaggtt caaaattatg
     2641 tcacgagtta taaattatga ctatcctgcc tatttcggct ttttcagata attatatctg
     2701 gacctttatt gataaaatag ctggcgtatt agattgtgtc gatccgggag aagcaggacc
     2761 aataatacgc tttgcccaat ccaaccagct gacactaaga acgatattat taacccacca
     2821 ccactatgat catataggag gtgttgactt gctaatcaag caatggccat cttgtaaggt
     2881 ctatggcccc atagatgaac gaatcaacaa tgtaacccac ccaattaaac aaggtcaatc
     2941 tgttcaagtg ggctcccttc actttcatat tttatttaat cctgggcata cttctaccca
     3001 tatcagttat tatgaaccac aacaaggatg gttattttgt ggagatactc tattttctgc
     3061 tggttgcggt agagtgtttg atggaactat agaggaattg catgaatccc tgctcttatt
     3121 taaaaaactc ccgcgaaata ctaaagtttt ttgtgctcat gaatacacct tacagaattt
     3181 aaaatttgca cataccgttg agccctgcaa ttcatcagtc atcaattaca tgcaacaaat
     3241 tctgaaacaa ccatcaccat gtactctccc gtctaatata gacttggaat tatcaattaa
     3301 tcctttttta cgcactgata aagagcaagt aaaacaatat gctctctcgc atggtgcgaa
     3361 ttcttcagat tctctggacg tttttaaggt attgagaaat caaaaaaact cgttcaaata
     3421 atacaacaaa ggatcaaata aaaacctatt tgcacccaat taaaccaaaa agggtttaat
     3481 ccttcattaa tttttagtaa actgattagc caattataat aacaggtaag accattgaat
     3541 tgtaatttat tgattataaa aagatttttt tcattcatcc ttatttttat tggtatttct
     3601 aacccggcaa tttctggcac agctccagat gtatgggatg tattacgtag tgaatttacc
     3661 ttaagccatg aagtaactcg cccagaagtg caagaacaaa tcagatggct aatttctcat
     3721 cctagttata ttaacaaagt aagtagccaa tctgaacctt acatttacca cgtgttaggt
     3781 gagataaaga aaagaaaact gcccggagaa ttggcgctac tccccatgat agaaagtgca
     3841 tatgatcctt ttgcctattc aggagcaggc gctgctggta tatggcaact catgcctcga
     3901 actggaagcg atcttggatt aaagcaagac tggtggtttg atggaagacg tagcattggc
     3961 ccttcaactg atgccgcatt aaattacctc gtctatctaa ataaatattt tagtggtaac
     4021 tggattttag ctattgccgc ttatgactct ggagaaggaa ccatttcacg ggcgctcaaa
     4081 tcaaccgcac gtaacgggcg caaggtgcaa ttctggaatc tgccagttcc cagagaaact
     4141 aaggtatata tcccgcgttt attagctctg gcagaaatta ttaacaatcc aaaacggtat
     4201 aaaatatcac ttcccaatat tccctatctt ccttattttg aagaggtcaa tattggtagt
     4261 caaattgatt taaatcatgc cgctaaactc gcaggaatca gttataagga gttgattaaa
     4321 ctaaatcccg gttataatcg atggacaact gcaccttata agccatttaa attactaata
     4381 cctgctgaaa aagtcgaaag atttaacttg aatctatcga atatacccga agataagcgc
     4441 gtaaactgga ctaaacatca agtaaaaaga ggcgacagct tggatgctat tgcaaagaga
     4501 tatcacacaa cagtcaattt gataaaacaa ctcaatcaat taaccagcaa taaagttcaa
     4561 cttaatcaat ccctactcat tccgagcagc aaaaattccc cagtagtagc taaaatacct
     4621 ccacagagaa aaaaacctgc tactaaacag ctgacaccta tgaaaaatgt ccgcattatt
     4681 catatcgtac aaaataatga taactatcag aaaatccaaa aagcttatgg ggttgcggct
     4741 agagagatac aaaactggaa taaattggca aataacgcgc cattacgtaa aggccagcgg
     4801 ctggtcatat ggaaaagagt caagcaacct gttgagtata tagtaaaatc aggggatagt
     4861 ttgagcgtta ttgccaaaaa gcaccaaaca aaagtgaata ctattatcag tttaaatcca
     4921 ggacttaaaa aatcaatgcc ccttcgcttg ggacaaaaaa tattaattgg
//
LOCUS       DASLOE010000191         4935 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.192, whole genome shotgun sequence.
ACCESSION   DASLOE010000191 DASLOE010000000
VERSION     DASLOE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.192"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(299..697)
                     /locus_tag="U6309_002625"
                     /pseudo
     CDS             complement(299..697)
                     /locus_tag="U6309_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            783..1163
                     /locus_tag="U6309_002626"
     CDS             783..1163
                     /locus_tag="U6309_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506043.1"
                     /translation="MSKDIDPLSSVKESFSHWRTTRLRQGKIPDYLWEQVRGLLANYP
                     IAKICSTLSISTVQVREKLLPKEDVAIQFVEVKEPLSVVTIPTREQGNVCSIELHRPC
                     GSIFKVHTLPVSLVSQLITDFMRA"
     gene            1160..1513
                     /gene="tnpB"
                     /locus_tag="U6309_002627"
     CDS             1160..1513
                     /gene="tnpB"
                     /locus_tag="U6309_002627"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEN5506044.1"
                     /translation="MMLQITPQHRILLAVEPIDFRNGVDGLKAICRQKLCDNPFNGTV
                     FAFRNKSGTSVKILVYDATGFWLCQKRFSKGRLAWWPSGASGSVKVRSSELMILLAQG
                     NPTFARLPEDWLSLV"
     gene            complement(1510..3144)
                     /locus_tag="U6309_002628"
     CDS             complement(1510..3144)
                     /locus_tag="U6309_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN5506045.1"
                     /translation="MTPAKELPIVIEKSSDEVEQIVALVHSSNLPEETKPFVIGCINL
                     ASWIPRALVEHKITVSNLKRLIFGKGDKATKQHDKSPKAEQNNTAPEEPAPSASNDEN
                     HEPKEPKGHGRLPHTAYINANEHTIALESLSSGQPCPLDCGGKLYSINPGILINIKGQ
                     NLASVDKYWIEKLRCALCNEVFSADIPTHVHQEKYHPSFRAMLALQKYYMAMPFHRQE
                     YFQSLIGFPIPASTQWQLMEELAGCALLVFPALEILAANGSLIHNDDTVLRIVDTIHH
                     NRQNPDKKRTGMYTTGVLAQNGAYKIALFYNSQRHSGENMERLLHKRHKNKGKVIQMC
                     DALSHNIPETHDTIVCNCLSHGFRKFDELQTFYPEHCLPIMKWLSIPFKRDEESKQLG
                     HDEEQRLIYHQKHSLPAMLEAKVYMEHLLSSKKVEPNESLGKAIKYMLRHWEKLTRFL
                     HIPGAPIHNNDMERGLKIPIRGRNTWLFYKTEYGAMVGGVLTSIIYTCELAGANPFDY
                     LVALQVYKDHIFKEPSLWLPWNYQYSKQSLGSAIAA"
     gene            complement(3251..3379)
                     /locus_tag="U6309_002629"
                     /pseudo
     CDS             complement(3251..3379)
                     /locus_tag="U6309_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947823.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain protein"
     gene            complement(3923..4150)
                     /locus_tag="U6309_002630"
     CDS             complement(3923..4150)
                     /locus_tag="U6309_002630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506046.1"
                     /translation="MKFYLHPSCHLLSMHWNSFDLIKASRDGYKHPPRPKKMAHSQSL
                     VIWRRQLQIRYYLMDKLEQNMLSQSKTRLLS"
     gene            complement(4308..4829)
                     /gene="msrB"
                     /locus_tag="U6309_002631"
     CDS             complement(4308..4829)
                     /gene="msrB"
                     /locus_tag="U6309_002631"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN5506047.1"
                     /translation="MKSIIKGLLFFFMSVASLTAYAEQAYKRPSDAVIKQKLSTIQYN
                     VTQQQGTESPFNNAYWNNEEQGIYVDVVTGEPLFISLDKYDSKTGWPSFTKPLEPGNI
                     IYKDDSSMFTSRTEVISKHGKSHLGHVFGDGPPPTNKRFCMNSAALEFIPVKDLQKRG
                     YGKYLPLFKNAGK"
BASE COUNT         1362 a          954 c         1056 g         1563 t
ORIGIN      
        1 atataagtat ccccagaaat caataggtgt tttttcgaaa atatcattgt aacaattata
       61 taggaagctg gtataagctt tcattctgga ttgggcattc attatgttgc tttaatccta
      121 acatcaccta aataaaagta tagatcaaaa attgatcact tgaggttgtg taatgatttg
      181 cttttatttt tgtagattta aggtatgaaa aaagatatcg aataaagtga gagaacctct
      241 ggtttcgagt aaagtttaaa ctaactcggg atcagaggtt ctcgctattt gaggcggatt
      301 atagtttaac ccacatatct gaatccaagg actcctcttc ctcatgctta acaacatatt
      361 gttggtgcgg tgcatgttgt gaagctgagt tcagtgtatt gatgcaatgt gatacgtcag
      421 tgttgttaag ccaatgttct tgtaaattgt agctcttcgt accgattaat ctatctcctg
      481 ttttttcaag aagggttttg gagtagttta ccagactgtt agccgcgtca actcccaagt
      541 catcttgaag tggtttggtt acctttttcg cgaattcctc tttagaatag catcttaaca
      601 aatccagaca atgacattgt ctcattattt tagctatgat tgcctgttct ggtttgttca
      661 cttcgggatg aaaaccaggg tttccataat cgagcaccct ggactttcgg caaccgtgcg
      721 tgactaatga cagcacgatt agttagccta tagcctcatt aatttcaaga ggctacaatc
      781 ccatgagtaa agacattgat cccctctcgt ccgtgaaaga gtcattctct cactggcgaa
      841 ccacccgatt aaggcaaggt aaaatccctg attatctatg ggaacaagtc agggggcttt
      901 tggctaatta tcctattgca aaaatctgca gcacactaag tatcagcact gttcaagtca
      961 gagagaaact actccccaaa gaggatgttg ctattcaatt tgttgaggta aaggaaccct
     1021 tgtctgttgt gactattccg acccgcgagc aaggcaatgt gtgtagtatt gaacttcacc
     1081 ggccgtgtgg aagtattttc aaagtacata cgctgcccgt cagcttggtg tctcagctga
     1141 ttaccgattt tatgagagca tgatgcttca gataacccca caacatcgga tattattggc
     1201 ggttgagccg attgatttta ggaacggggt tgatggctta aaggccattt gccgtcagaa
     1261 gttatgtgat aaccccttca atgggactgt ttttgctttt aggaataaaa gcggcacgtc
     1321 tgtcaaaatt ctggtttatg atgcgacagg cttttggctt tgccagaaac gctttagcaa
     1381 aggccgtctt gcatggtggc caagtggagc aagtggctct gtgaaggtcc gttccagtga
     1441 gctaatgata ttgctggctc agggcaatcc cacctttgcc agattgccgg aagactggct
     1501 tagccttgtt taagctgcta tggcagaccc aagtgattgt tttgaatatt ggtaattcca
     1561 cggaagccac aggcttggtt ctttaaaaat atggtctttg tacacttgta aagccaccag
     1621 ataatcaaat gggttggcgc cagcaagttc acaggtataa atgatgctgg tcagcacacc
     1681 accgaccatg gcaccgtatt cggttttgta gaataaccag gtgttgcgtc cacgtatggg
     1741 tatttttaat cctcgttcca tgtcgttgtt gtggatgggt gcaccgggaa tgtgtagaaa
     1801 gcgggtgagt ttttcccagt gtcttagcat gtatttgatg gctttaccca atgattcatt
     1861 cggttctact tttttggacg acagaagatg ctccatgtaa actttggcct caagcattgc
     1921 gggcaagctg tgcttctggt ggtagatcag acgctgctct tcatcatgcc ccagttgttt
     1981 tgactcctca tccctcttaa atggaattga aagccatttc atgatgggca aacagtgctc
     2041 cgggtaaaat gtctgtaatt cgtcaaattt acgaaatcca tgagataagc agttgcacac
     2101 aatagtgtca tgggtctctg gaatattatg gcttagcgca tcacacattt ggatgacttt
     2161 tcctttattc ttatggcgct tatgaagaag gcgctccatg ttttcaccag agtggcgttg
     2221 gctattataa aagagtgcta ttttataggc gccgttttgt gccaatactc ctgtggtgta
     2281 catgccagtg cgttttttat cggggttttg tcggttgtga tgaatcgtat ctacgattcg
     2341 aagcactgta tcgtcgttat gaattaaaga gccattagcc gcgagtatct ctaaggcggg
     2401 gaataccaat aacgcacacc cagccagttc ttccatcagt tgccattggg tagatgccgg
     2461 aatggggaag ccaatgagtg attgaaagta ctcttgccga tgaaagggca tggccatgta
     2521 gtatttttgt aaggccagca tggctctaaa acttggatga tatttttcct ggtggacatg
     2581 agttggaata tcggctgaaa agacttcatt acataaagcg cagcgtagct tctcaatcca
     2641 gtatttatca acggatgcta gattttgtcc tttgatgtta atgaggatac cgggattgat
     2701 ggagtaaagc ttgccaccac agtcaagtgg gcacggttgg ccagaactca gggattctaa
     2761 ggcaatggtg tgctcatttg cattgatgta agcggtatgc ggaaggcgcc catgcccttt
     2821 gggttcttta ggctcatggt tctcgtcatt tgatgcacta ggtgcgggct cttctggggc
     2881 agtattattc tgttccgcct ttggggattt gtcgtgttgt ttcgtagcct tatccccttt
     2941 gccaaagata agccgcttta aattggatac agtgatttta tgctcgacca gtgcgcgagg
     3001 aatccaggag gccaggttaa tgcacccaat gacaaagggt tttgtttcct caggtaagtt
     3061 tgatgagtgc accagtgcaa cgatttgttc tacttcatcc gatgattttt caatgacaat
     3121 gggtaattct ttggctggtg tcatttagca accctgcttc tcttaagggc agcaagatta
     3181 actatcaagc gtaataaaat caataggttg tagtgaaaat gctgatttaa tgcttttttg
     3241 ctgcaaagtc acccaccaaa ctgtaggaaa tatctgaagc ccaggcaaga actggcatca
     3301 taaaaataaa tattaattgc ggtatataca atgatttaat ttttttcatc gctgctatcc
     3361 ttataaaatt gttaaccata tttaaatctt tttacattga agtggtacgg aaaatctttc
     3421 cctggacttg cacctgccgc ataacaaagc ggcgcttttg ttgccaaaaa aattgcggct
     3481 cttgtttata ataaaccatc caatgctact tttaattaca cacatttagg cagcctggcg
     3541 accattatta gaaaagcatc aatagttgaa ttaaatcatt tcagctttta tggagcgctt
     3601 gcctggtggt tttgaggagt tgtccatgta tccttccttg taggagcacg aaataaattc
     3661 tctgtgctgt ccgattgagt atggtccttt tttactttta gagcaaataa tttacttatt
     3721 acctccatta agaaaaagaa atacagatag aaaaaaccaa tgattttaat gaatgactag
     3781 aaacgaggca taatagtgtt ttagacaaaa ggattcaatt gatggacaaa caattgttcc
     3841 tcaagccaaa catacaattt ttttaccaaa tagcttgccg ctttctcatc cttcatttca
     3901 tagcttaatt gttcacaaat ttctatgaaa gaagccttgt tttggattgg cttaacatat
     3961 tttgctctaa cttatccatt agataatatc ttatttgaag ttggcggcgc caaatgacta
     4021 aagattgtga atgagccatt tttttaggac gaggaggatg cttgtaacca tctcttgatg
     4081 ctttaatgag gtcaaaactg ttccaatgca ttgataataa atgacaagat ggatgaagat
     4141 aaaacttcat gtcaggttta agctccctgt tgataatatt atccagctta aaggtagctg
     4201 attaattgat aaattgcaaa aaccaggcaa aaccactcaa ctgcctaagc tctgtttatc
     4261 aaaggcaaat cttgttgtga gccataaaca tggcatatta cttcacgtta ttttcctgca
     4321 tttttgaata gtggcaaata cttaccataa cctcttttct gcagatcctt gactggaata
     4381 aattccagcg ccgcagaatt catgcaaaaa cgtttattgg tgggcggagg gccgtcacca
     4441 aaaacatgcc ccaaatgtga ttttccatgt ttggaaatca cttcagtccg actagtaaac
     4501 atgctgctat catctttata tattatgttt ccaggttcta gcggtttggt aaaactcggc
     4561 cagccagtct tagagtcata tttatcgagc gaaataaata atggttcacc ggtaacaaca
     4621 tcaacataaa taccttgctc ttcattattc caataagcat tattaaacgg ggattctgta
     4681 ccctgttgtt gagtaacatt atattgaatt gttgataatt tttgtttgat gacagcatct
     4741 gaaggtcgtt tataggcttg ttctgcatag gctgtcaaag aggcaacact catgaaaaaa
     4801 aataacaaac ctttgataat actcttcatg gtggtttctc actgattaat gctctattct
     4861 taagtatagt cagaattccg aacaacagaa ttccgaacaa ctgtcaattt ccttgacgta
     4921 agttatatgg tctgc
//
LOCUS       DASLOE010000192         4929 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.193, whole genome shotgun sequence.
ACCESSION   DASLOE010000192 DASLOE010000000
VERSION     DASLOE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.193"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            8..1342
                     /locus_tag="U6309_002632"
     CDS             8..1342
                     /locus_tag="U6309_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2525 family Dot/Icm T4SS effector"
                     /protein_id="HEN5506048.1"
                     /translation="MKEKYKKSLYIPNELLLEIFADKNLDIPNIIVVSMVCKLWYELS
                     LNNAVWRPLFIKRFSYSPTLANHSIFKEGYQKQLALTQAMKKKCESNHVCRNEPGLLE
                     AIREKNIPLIYALFESGHQIIIDSFSDDEQQFAEEMLVFLSECAELPSSLLTRIFKRG
                     FCTANMETVEFLALDFIVAEADEKLLQKIFRALGDDLLSVSRYCDFDDNNFFSSLLHF
                     PVERFSLVLRLLYETLGKTKLSTLLVYQNHTYTDWSCIHSASRRGGEYFFIALKYLKR
                     QDYPVLLKKGLNDEDIPLFNIAYWGDKESLSHIINILGADATTFLKYKDNKGSNILLP
                     ALLSEDHSIVELIEKVMGMEFIDLLLMQDTNGRDILRRVMLLQEPTVLRRINTVLKLL
                     RDYDCLDIAQELYSSDEFTQLISQNLYINQDEAIELFNKYTEEQTNNFCSIL"
     gene            complement(1623..2993)
                     /gene="mavL"
                     /locus_tag="U6309_002633"
     CDS             complement(1623..2993)
                     /gene="mavL"
                     /locus_tag="U6309_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavL"
                     /protein_id="HEN5506049.1"
                     /translation="MGLRLFQYNQHIYRGRFSVSNAYCKDFVVRNINTIFGLSENIMA
                     YQLLLSKETLNKILQYKQNLEKGLVTPGKFFLEELSKQEKSISEMDITTFTQLLIQSK
                     KPQVFAESQVYHDGTDWTLEEESILGDVSVNMPVTMYNDGGHGSSFKNHPKPISGYLA
                     YVPGALLASGSGPTSDMKEVLDSGKLNQDKLNALYERRLLPQLIHFNELARQNEKQAA
                     ITIPGIGTGCFSGAYYDVIKPYVRNALIHILEKHKDSLPYIDIIHYDPYMGDEPAEKK
                     IGHMSFRVSPSGVVRGTTGQLDYPLGSNPDTHILVSIVAWDHFSWPGNDYWGGARQTD
                     DGVKAASTDTMGQVTGATGVYDKKWGRYMPPESFTKDRKGMSDWGDYVRENGIVFNGP
                     VLALDKSGKLDTLENVASRSKAKIDTTATISELVRGMFSLFSHSTTTELSPTVKEEES
                     KKSGPQ"
     gene            complement(3333..>4929)
                     /locus_tag="U6309_002634"
     CDS             complement(3333..>4929)
                     /locus_tag="U6309_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lpg2527 family Dot/Icm T4SS effector"
                     /protein_id="HEN5506050.1"
                     /translation="ISLSLDSPKLDESDFILLSVKYLEKSLGKKKEFSVFFEDIEKLY
                     FKQNYKEAIEKILDFCKKNESLLSEQVVQRLAEVAPRLKSNPKDNESRRLYETLYADH
                     LESVIKQESDLSVFNELRDSYNAVKPEYAVTHETEIKTLDEAKQFILSFVMLNDNVEL
                     PLKAQSERYPKKDRSREELGNTLSANPGIMKPNSPNFTDNLVPVRDVPKIAINEKVVG
                     GYSKTKPTTPFVASLSGTTYSLMVVLTDYIEKHKTDKDIEKKVNQIINLWISSYIKEG
                     YHSYSEVVDVLTEPFLQSIFDKANIKLNYGVLDDTHAEFRKAQDYVFGLTIRSAMHHE
                     LQERFKNKEKLQEEVKNFESGLNKLNQNVENTGRHREALNKLNEVFQDWSAGKKSYDA
                     FKSESNQLIHDIESEEQKSNRGLGSMLKNLGNYLLYLITLRFLKEDYPKPSSAVTMLV
                     TELRGRLEQIDHFHKTSLSLKKATQPDPKNTSPSGETTDVLKSAVDTDKLNKKQDSEV
                     KKTLDSEQLEEVQRQDDKIARLH"
BASE COUNT         1530 a          938 c          837 g         1624 t
ORIGIN      
        1 aaactatatg aaagaaaaat acaaaaagag tctatatatt ccaaatgaac ttcttttaga
       61 aatttttgcg gataaaaatt tagacattcc aaatatcata gttgtcagca tggtttgtaa
      121 actttggtat gagttatcac ttaataatgc cgtttggagg cctttattta tcaaacgatt
      181 ttcctattca ccgactttag ctaaccactc catctttaaa gagggttatc aaaaacaatt
      241 ggctttaact caagcaatga aaaaaaaatg tgaatcaaat catgtatgca ggaatgagcc
      301 aggattacta gaggctatta gagaaaaaaa cattcctttg atctacgcct tattcgagtc
      361 tggccatcaa attatcatag atagttttag tgatgacgaa caacagtttg ctgaagaaat
      421 gcttgttttt ttatcagaat gcgctgaatt accaagttcc ttattgacaa gaatttttaa
      481 acgtggtttc tgcacagcaa acatggagac cgttgagttt ttggcattag atttcattgt
      541 tgcagaagct gatgaaaaac tattacaaaa aatatttaga gctctaggtg atgacttatt
      601 atcagttagc cgctattgcg attttgatga taacaatttt tttagctctc tattacattt
      661 tcctgtagaa cgattttcat tagtactgag attgctctac gaaactttag gaaaaacaaa
      721 actgagtact cttttagtgt accaaaacca tacttacact gattggagtt gcattcattc
      781 tgcaagtaga cgtggtggag aatatttctt cattgcgctc aaatatctta aaaggcaaga
      841 ttatcctgta ttattgaaaa aaggattaaa tgatgaagat attcctttat tcaatattgc
      901 atattggggc gataaagagt ctttatctca cattattaac attttgggag ctgacgcaac
      961 aacgtttctt aaatacaagg ataataaagg aagtaacatc ttattacccg cattgttgag
     1021 tgaagatcat tcaatagttg aattaattga aaaagtgatg gggatggaat tcattgattt
     1081 gttacttatg caagatacaa atggccgcga tattttgaga cgagtcatgt tacttcagga
     1141 acctacagta ttgaggcgca tcaatactgt acttaaacta ttacgtgatt atgattgcct
     1201 ggacattgct caggagctat atagcagtga tgaatttacc caactcatat ctcagaattt
     1261 atatataaac caggatgaag ccatagagct attcaataaa tatacagaag aacaaacaaa
     1321 caatttttgt tctattttat aaaattagaa taaatatatg aacgtacttt atcattcaaa
     1381 aagattcgaa gttgcatctc gaaataatag atggatcctg ccaatttaaa aatgcctcgt
     1441 aataagccag aatgagattg ttacgattta ttcaaaatag cagaggctcc atactattat
     1501 tcaatctttc acgaaaccac acataacttg accacgtcaa gttgttaagc cctgcagata
     1561 gtaatatggt ccaacctcaa aagtcagacc tattgttact ctataagaat ttgtcaaaat
     1621 tattactgag gacccgattt tttcgattct tcctctttaa cagttggaga aagctctgtc
     1681 gtagtactat gggaaaaaag gctgaacatg ccgcgaacga gctcactaat tgtggctgta
     1741 gtatcaattt ttgctttgga acgacttgca acattttcca aggtatccaa tttaccgctt
     1801 ttatccaaag ctaaaacagg gccattaaat acaatgccat tttctcttac ataatccccc
     1861 cagtcactca tacctttacg gtctttagta aatgattctg gaggcatata tcgaccccat
     1921 tttttatcat agacaccagt tgcgcctgta acttgcccca tggtatccgt gctagcggcc
     1981 ttgacaccat catctgtttg acgagcgccg ccccaataat catttccggg ccaggaaaaa
     2041 tgatcccagg caacaattga aacgagaata tgcgtatctg gattactacc taaggggtag
     2101 tcgagttgcc ccgttgtgcc acgaaccaca cctgaaggac tcacacgaaa agacatatga
     2161 ccaatcttct tttcagcagg ttcatccccc atataagggt cataatgaat gatatcaatg
     2221 taaggtaacg aatctttatg tttctcaaga atatggatta atgcgtttct aacatagggt
     2281 ttaatcacat cataataagc gcctgaaaaa caccctgttc ctataccggg aatagtaatt
     2341 gctgcttgtt tctcattttg ccgcgccaac tcattaaaat ggatgagttg aggcaacaaa
     2401 cgtcgttcgt acaaagcgtt taatttgtct tgatttagtt tgccgctatc aagcacttct
     2461 ttcatatctg aagtcggtcc actgcccgaa gccaagagag cacctggcac atacgccaaa
     2521 taaccggaaa ttggctttgg gtgattcttg aaagaggaac catgcccacc atcattatac
     2581 atagtgactg gcatattgac actaacatcg ccaagaatgc tctcttcttc cagtgtccaa
     2641 tcagttccat catgataaac ctgactttca gcaaaaactt gtggtttttt agactggatt
     2701 aatagttgag tgaatgtagt aatatccatt tcggagatac ttttttcttg tttacttaat
     2761 tcttcaagaa aaaatttccc gggcgtgaca agcccttttt cgaggttctg tttgtattgt
     2821 aaaattttat ttagggtttc tttactgagc aataattgat aggccattat attctcactt
     2881 aatccaaaaa ttgtattaat gtttcttacc acgaagtcct tacaataagc attgcttact
     2941 gaaaatcttc ccctatagat gtgttgattg tactgaaaca atcttaaccc tattgcctca
     3001 ataaaagcaa tttaattggt caatatggat ataataataa cacacaatga ctgctattcc
     3061 aatcagtgaa aacatgctat gccgttttca aaactggaat atggcttgat caatatataa
     3121 ctgcctagtt ttattgcaaa tactttttta gtctataaat ttataattaa atgcatataa
     3181 cccatgctca ttagtcttct tgcccaagct gattattttg ttttagggca aggcaaggcg
     3241 caaacgtttt gaggagagaa gtttacatac aggcacttca gaatcatttg caacgcggca
     3301 ataaaatgaa agaagcaggt tatgcaacag ctctaatgta aacgagcgat tttgtcatct
     3361 tgcctttgaa cttcctccaa ttgctcagaa tctaatgtct tttttacttc actgtcctgt
     3421 tttttattaa gcttgtcagt atcgacagct gatttcagaa catctgttgt ctcaccagat
     3481 ggtgaagtat ttttaggatc aggctgcgtt gcctttttta aagagaggct ggttttatga
     3541 aaatgatcta tttgctcaag tctgcctctt aattcagtga ctaacattgt gaccgcacta
     3601 gacggttttg gatagtcttc tttcaaaaaa cgaagagtaa tcaagtataa aagatagttc
     3661 ccgaggttct tcagcatgga gccaagacca cgattacttt tttgttcttc agattcaata
     3721 tcatgaatca gttgattaga ctcactttta aaagcatcat agcttttttt gcccgcagac
     3781 caatcctgaa atacctcgtt taatttattc agtgcctcgc gatggcgacc tgtattttcg
     3841 acattctggt tgagtttatt taacccagac tcaaaattct ttacctcttc ctgaagtttt
     3901 tctttattct taaatcgctc ttgtaactca tgatgcattg ctgatcgaat tgtcaaacca
     3961 aaaacataat cctgagcttt acgaaattct gcatgcgtgt cgtcaagtac accataattt
     4021 aatttaatat ttgctttatc aaaaatggat tgaagaaacg gctctgtcaa aacatccaca
     4081 acctcactgt agctgtgata accttccttg atataactgg aaatccataa attaataatc
     4141 tggtttactt ttttttcaat gtctttatct gttttatgct tttcaatata gtccgttaac
     4201 accaccataa gtgagtaggt tgtgccagac agcgacgcca caaaaggagt agtaggctta
     4261 gtttttgaat acccacccac cactttctca ttaatggcta ttttgggcac atctctgaca
     4321 ggcaccaggt tatcggtaaa attaggtgag ttaggtttca taatccctgg attcgcagat
     4381 agagtattcc ccaattcttc cctggatctg tcctttttgg ggtatctctc tgattgtgct
     4441 tttaaaggta attcaacatt atcattcagc atgacaaaag ataaaataaa ttgctttgct
     4501 tcatccaaag ttttgatttc ggtctcatga gttactgcat actcaggttt caccgcatta
     4561 taactgtctc tcaattcgtt aaatacggaa agatcactct cttgtttaat gactgattca
     4621 aggtgatctg catacaaggt ttcataaagc cttctacttt cattgtcttt aggattgctt
     4681 tttaatctgg gagcgacctc tgccagcctc tggacaactt gctcacttaa caaggattca
     4741 ttttttttac aaaaatccaa tattttttca attgcttcct tataattctg cttaaaatag
     4801 agcttttcta tgtcttcaaa aaaaacagaa aactctttct ttttgccaag tgatttttca
     4861 agatacttaa ctgagagcaa tataaaatca gactcatcta attttggaga atctaatgat
     4921 aatgaaata
//
LOCUS       DASLOE010000193         4918 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.194, whole genome shotgun sequence.
ACCESSION   DASLOE010000193 DASLOE010000000
VERSION     DASLOE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.194"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            3..704
                     /locus_tag="U6309_002635"
     CDS             3..704
                     /locus_tag="U6309_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506051.1"
                     /translation="MKRFFSILFILYCCNLFAMAPKLNWDKAIDKAIKRYGLRVEPQL
                     ISYFSKAGVSYPPKEIALLAFKSERKVELWAKNPSQEWTHIHDYPLTGFSGRLGPKLR
                     ENDKQIPEGIYKLVNFNPFSSMHLSMMINYPNSFDRQKGYMDGRKELGNNIFIHGKNL
                     SVGCLAVGDLAIDQLFILARRVGLKNIQVIIAPNDLRYQKPSTSTFAQPRWLPELYKQ
                     ITESLKPFKKTKFTA"
     gene            799..2955
                     /locus_tag="U6309_002636"
     CDS             799..2955
                     /locus_tag="U6309_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEN5506052.1"
                     /translation="MNTPRRQSWSHIDLGELAKNDSLLACLVLLTRYYQNPFSPPSLI
                     ARLPLVNSKLSVDMFSRAAARASLDSEVKTLKLEQITDSDIPLVLIKKNGEAVLLILE
                     DDGKKAILDPKQPDSRIDLNQIADELSGEVIFVEQQYKPSTRAHETLSKEQKNWFWKV
                     VIKSWPLYSEVLVASLLVNIFALVVPLFSMNVYDRVVPNHAIETMWVLASGVALVFLF
                     DMLLKSLRSYFIDVANKHSDKELSALIFQQVLGLSMANRPKSVGSLANTVQSFEVFRD
                     FITSTTMTVLVDLPFVFIFLFVIYLIGGVLFWIPFLVLPTIFIIGLLLQWPLIRLTRK
                     SYQYGAEKQATLFESLATIETVKTTGAESVLQSRWERIINESSKNSMQLRFVANTSIS
                     ITNLAQQIATIAIIIAGVYMISEGELTTGGLIACTILTGRALAPMAQVSTLFTRYFQS
                     VNALDSLNKIMQLETDTTEETRYLHRPSLKGAVEFKQVSFHYKEDETKVLQNLNFKIN
                     AGERVAIIGRVGSGKSTLGKLILKLYVPTEGTILLDGTDYKQINPDDLRQQIGYVPQD
                     IVLFYGSIRDNICVGAPFINDSALLEAAEIAGVTSFTNNHPKGFDRQVGEKGAELSGG
                     QRQSVALARALIMSPNILLLDEPTSAMDDNNEKQIKKNLNEYLSDNRTLILITHKISM
                     LELVNRIIVLENGKIIADGNKEAVLSALRTGVTVRK"
     gene            2959..4095
                     /locus_tag="U6309_002637"
     CDS             2959..4095
                     /locus_tag="U6309_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEN5506053.1"
                     /translation="MKKTHSSKTFTHIILWTSLLFFIVAIIWANYAILDEVTTGQGKV
                     IPSSEVQVIQNLEGGIIQNIFVKEGQIVKKDQILMQIDNTRFMASYAEAEKKIDALEI
                     EIIRLNAEISDTKPVFPDKFIKTYPHLVQDQLSLYESRMRELNQLKKSLELAQKELDL
                     TRPLLKGGSVSEVEVIRLERSVSEIKGNIEKFKSEELDKLNKARTELFALVEANKADK
                     DRLTRTTVRSPVYGIVKQIKTTTIGGVVQPGSDLLEIVPLDDTLLIEAKIRPSDIGFI
                     HPGQKAMVKITAYDFSIYGGLEGVVEHISADTIIDEKTDKKEESYYIVKVRTEKNYLG
                     TEKKPLPIIPGMQATVDILTGQKSVLQYLLKPILKAKQSALRER"
     gene            4231..>4918
                     /locus_tag="U6309_002638"
     CDS             4231..>4918
                     /locus_tag="U6309_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN5506054.1"
                     /translation="MPSIVLPKLSGSYYISLYLTIVFSTLTLLLGLSVIIGWHSGIVF
                     LIQYYPNTVAIVYNTALCFIALSFSIFLFLNCHYKSAIILSLAVFILSGLALAQHAYS
                     INLGIDEIFFHHYQSIQNAYPGRMAANTAFCFLLIATALILMNFTSNHSLNNSLAGAL
                     SLLVFCLGVLFISGYFSDIQQAYKWGNKTPMSINTGVGFLLLSLAMMGLYWHNNIVYQ
                     LNSSIVMPYLS"
BASE COUNT         1544 a          827 c          939 g         1608 t
ORIGIN      
        1 ctatgaaacg atttttttcg atattgttta ttctttactg ctgtaattta tttgcaatgg
       61 cacctaagct taactgggat aaagcgattg acaaagcaat aaaacgttat gggctaagag
      121 tagaaccgca attaatttct tatttttcta aagccggggt tagctaccct cctaaagaaa
      181 ttgccttgct tgccttcaag tcagagcgta aagttgagtt atgggcaaaa aatccaagcc
      241 aggaatggac gcatattcat gattacccat taactggttt tagcgggcgt ttaggaccca
      301 aattacgtga gaatgataag cagatccccg aagggatcta taaattagta aattttaatc
      361 cttttagcag catgcactta tccatgatga ttaactaccc aaatagtttc gatcggcaaa
      421 aaggttatat ggacggtagg aaagagttag ggaataatat ttttattcat ggaaaaaatt
      481 tatctgtcgg atgtttggct gtcggtgacc ttgccattga tcaactgttt atcttggcaa
      541 gaagagtcgg cctcaaaaat attcaggtta ttattgcccc taatgactta cgatatcaaa
      601 agccatcaac atctacattt gcccaaccca gatggttgcc cgagctttat aagcaaataa
      661 cagaatcact caaacccttt aaaaagacta aatttactgc ttaatataaa gattggtctt
      721 tttattttat tttatttaag aaatttgagt atatacttaa attaaataaa taatataaaa
      781 aataatcata aaaatgatat gaatactccg agaagacaat catggtctca tattgatttg
      841 ggtgaactag ccaagaatga ctctctgcta gcctgcctgg tattattaac tcgatattat
      901 caaaatccgt tttcgcctcc atcacttata gctcgtttgc cattggtcaa tagtaaattg
      961 agtgttgata tgttttcgcg tgccgctgct agagcctctt tggattctga ggtgaaaact
     1021 ttaaaattag aacaaatcac tgattcagat ataccattag tactaattaa aaaaaatggc
     1081 gaggctgtgc ttctaatact ggaggatgat ggtaaaaaag caatccttga tcccaagcaa
     1141 cctgattccc ggattgattt gaaccaaatt gctgatgagt taagcggtga agtcattttt
     1201 gttgagcaac aatacaaacc tagcaccaga gcacatgaaa ctctttccaa agaacaaaaa
     1261 aactggtttt ggaaagttgt tattaaatct tggccattgt attccgaagt tcttgtggcc
     1321 tctctgctcg tgaatatatt tgcactcgtt gttccattat ttagcatgaa tgtgtatgac
     1381 cgtgtggttc caaaccatgc tattgaaacc atgtgggtgc ttgctagtgg tgtagcttta
     1441 gtatttctct ttgatatgct gctcaagagt ttacgatcct atttcattga tgtggcaaat
     1501 aaacacagcg ataaagagtt gtctgcttta atatttcagc aagttctcgg attaagtatg
     1561 gctaacaggc caaaatcggt tggttcttta gccaatacag tacagtcatt tgaagtattc
     1621 agagatttta ttacttcgac tactatgaca gttcttgttg atcttccttt tgtatttatt
     1681 tttctttttg ttatttattt gattggcggg gtgttgtttt ggataccttt tttagtgctc
     1741 cctaccattt ttataatagg tttattgctg caatggcctt taattcgact tacaagaaaa
     1801 tcctatcaat atggtgctga aaaacaagcg acgttgtttg agtctttagc tactatagaa
     1861 actgtaaaga caactggagc agaatcagtc ctgcaatcac gatgggaaag aataattaat
     1921 gaatcttcta aaaactctat gcaattacgt tttgtagcca atacaagtat cagtatcact
     1981 aatctggccc aacaaattgc gactatcgct attatcattg caggtgttta tatgatctct
     2041 gaaggtgagt taaccacagg tggattaatt gcctgtacca tattgaccgg aagagcatta
     2101 gcccctatgg ctcaagtatc cactctattt acccgttact tccaatcagt taatgctctg
     2161 gactcattaa acaagattat gcaacttgag acagatacca cagaagaaac tcgttatctc
     2221 catagacctt ctctgaaagg tgccgttgaa tttaaacagg tgtcttttca ttacaaggaa
     2281 gatgagacga aggtattgca aaatctcaat tttaaaatta atgctggtga gcgcgttgca
     2341 attataggac gagttggttc tggtaaatcg actttgggta aattaatatt aaaactgtac
     2401 gttcctaccg aaggaactat tttactggat ggtaccgatt ataaacaaat taatcctgac
     2461 gatttacgtc agcaaatagg ctatgtacct caggatattg ttttatttta tggaagcata
     2521 agagataaca tttgtgtagg tgctcccttt ataaacgact ctgctctttt agaagccgct
     2581 gaaatagctg gggtaacgag ttttaccaat aatcatccca aaggctttga taggcaagta
     2641 ggagaaaaag gagccgaatt atcaggagga caaagacaat ctgttgctct tgctcgtgct
     2701 ttgattatga gccctaatat cttgttattg gatgagccta cttcggcaat ggatgataat
     2761 aacgaaaagc aaatcaagaa aaatcttaat gaatacttat ccgataatcg tactttgatt
     2821 ttaataactc ataagatttc catgctggag ctggtcaatc gtattatcgt tttggaaaat
     2881 ggcaaaatca ttgctgatgg taataaagaa gctgttctaa gcgcccttag gactggggta
     2941 acggtgagga aatagtctat gaaaaaaact cacagctcaa aaacctttac ccatattatt
     3001 ttatggactt ccttgctttt ttttattgta gcaattattt gggctaatta tgccattctt
     3061 gatgaagtaa ctacagggca aggaaaagta attccctcta gtgaggttca ggtcatacag
     3121 aatcttgaag gcgggattat tcagaatatt tttgtcaaag aagggcaaat agttaaaaaa
     3181 gatcaaattt taatgcaaat cgataatact cggtttatgg ccagctatgc cgaagcagag
     3241 aaaaaaattg atgcattgga aatagaaatt ataaggctta atgctgaaat tagtgataca
     3301 aaacctgtgt ttccagataa atttatcaaa acctatcctc atcttgtgca ggatcagttg
     3361 tctctgtatg aatcacggat gagagagttg aatcaactga aaaaatccct agagttggcg
     3421 caaaaagaat tggatttaac tcgcccatta ttaaagggtg gatccgtttc agaagtagag
     3481 gttattcgtc tggaaagatc agtaagtgaa attaaaggaa acatcgagaa gttcaaatca
     3541 gaagaactgg ataaattgaa taaagccaga acagagttgt ttgcgctggt tgaggcgaat
     3601 aaagcggata aagatcgttt aacaaggacc acagttcgtt ctccagttta tggtattgta
     3661 aaacaaatta agacgacaac gattggtggt gtagttcaac caggtagtga tttacttgag
     3721 atagtcccgc ttgatgatac tttattaatt gaagcaaaaa tacgcccttc tgatattggt
     3781 tttatacacc caggtcaaaa agcgatggtt aaaattactg cttatgactt ttctatctat
     3841 ggtggattgg agggtgttgt agagcatatt agtgccgata ctattattga tgagaaaaca
     3901 gataaaaaag aagaaagcta ttatatagtt aaagtgagaa cagagaaaaa ttatctggga
     3961 actgaaaaaa aaccattgcc catcattccg ggtatgcaag ctacagttga tatacttaca
     4021 ggtcaaaaat ctgttcttca atatcttttg aagcccatac ttaaggctaa gcaatcagca
     4081 cttagagagc gttaattagt gtgtaaaaac attttggaaa tgctgtgtgt tacgattctg
     4141 tgagaatgtc gtgagcgtgc gataaataaa tagtctatta ttatattgat aacatgtttg
     4201 caagaaaatc actgccaggg atgttatatt atgccatcaa tagtactccc taaattatct
     4261 ggttcttact acatttcact ttatttaacg atagtttttt ccacactaac acttttattg
     4321 ggactctctg ttattattgg ttggcatagc ggtattgtct ttttaattca atactaccca
     4381 aatacagtag ccattgttta taatactgct ttgtgtttca tcgctttatc tttttctatt
     4441 tttttatttc taaattgcca ttacaagtca gcaattatat tgagtttagc tgttttcatt
     4501 ttatctggtc tagctctagc tcaacatgct tatagtatta atcttgggat tgacgaaata
     4561 ttctttcatc actaccagtc cattcaaaat gcttatcctg gcagaatggc tgcaaatacg
     4621 gctttttgct ttttattaat tgctacagca cttatcttga tgaatttcac tagtaatcat
     4681 tcacttaata attctctggc aggggcactt agtttattag tattttgttt gggagtattg
     4741 ttcataagtg gttatttttc agatattcag caggcttaca aatggggcaa caaaaccccc
     4801 atgtcaatca acacaggagt tggttttttg ttgctctctt tagcgatgat gggtttatat
     4861 tggcacaaca atatcgtcta tcaattaaat tctagtattg taatgcccta cttgagct
//
LOCUS       DASLOE010000194         4784 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.195, whole genome shotgun sequence.
ACCESSION   DASLOE010000194 DASLOE010000000
VERSION     DASLOE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.195"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..220)
                     /locus_tag="U6309_002639"
     CDS             complement(<1..220)
                     /locus_tag="U6309_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506055.1"
                     /translation="MSDKVILAIIYEAAQFFAFYELFCKLKEDKKFCFIIYCPYYLPE
                     TEQYKILCTKNNFFYIYDTNSQGGDADIL"
     gene            475..1194
                     /locus_tag="U6309_002640"
     CDS             475..1194
                     /locus_tag="U6309_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5506056.1"
                     /translation="MSTVAQYKNYYHYISDGWSKEPKEMFKFLAQKIKELPLKPGNKL
                     LDIGCATGELIHFLRHQFPQLHYTGIDVFDDLIEVCKELQPNVNFINASALDLPFEFE
                     NQFDIITVVSVISIFDTIDLDIFWDNLFKATKDNGVIYVLSPFNEYGVDCEIRHRKRI
                     NNIKGEWERGWNIFCKETIAEYLEGRCKNWFFHPFKFNLELNQREDVIRTWTMKTEFN
                     DRQLTNGMKLMVDHYLLEIHI"
     gene            1207..2292
                     /locus_tag="U6309_002641"
     CDS             1207..2292
                     /locus_tag="U6309_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEN5506057.1"
                     /translation="MSQPFTIGPFSIGENHPPIFLAEIGTFFNQDLGIAEQLLHKIIT
                     IRDKVPYQPLILKTEILNNPNICLPVDYEETYTSKSGEVRKENYRSLIERKILPLEHY
                     SMLFSLLKNANLPFIVSIYELDTIDFAIKQGACALKTSSSNIVHIPLIRHLAKTGLPL
                     IIDTGRSSLTDICKAVETAKNAGCEKIIIEHSPDGHPALPEAHNLRMLRTLNQCFSLP
                     VGLSDHYNGIEMLYMSIALGASILEKGVHLYPEDIDQDISHTMGIDQLAKTLETIYNC
                     WQALGQPMRDLETPIKGVLGSSQRTCLVAKQKLQPGSLISLDNTTFAFPCKGVPTQYW
                     DLVNGWELQNKVSIGEPIKWSDIKQKK"
     gene            2289..3290
                     /locus_tag="U6309_002642"
     CDS             2289..3290
                     /locus_tag="U6309_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEN5506058.1"
                     /translation="MIVRNPTWNSLTPPLPKFVSCNTVETDFLKEKLGEDGRVYIAQF
                     DLKSTSAFLFQRKSDQKKFFIKKVSSEHKRQYQQSEHLAQFIACPDYIVNIAINCVSN
                     EEENSLYYIYPYIHGKRLFAEPEEIISLATALAKLHLKKKSYPDQQLIIKNTTERTSQ
                     LNFIRKALADGCYSYIPHFSFVKKMAQQYDFDWINQEDAQPIHGDLNAGNLLLSEDNM
                     ICFFDFEDALHSFHPVVLDLLFVIERIIFNQNSSTEQLLNLGLMFIHAYKKAGGTYQY
                     KTRDEFGLTILALKAFCLLTLLAEKNKNILSSEWDKFLKLTQKAENERGLIKTILQG"
     gene            3301..4428
                     /locus_tag="U6309_002643"
     CDS             3301..4428
                     /locus_tag="U6309_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506059.1"
                     /translation="MRILVTARDVGAALNIIEIVKILKQYAGVTVFIYAQPPASKYFL
                     RAGIQPVFEVPLPPSRSLNEASDLLSYANQLIDKLNPDIILSGLSSPGEAGIDEAIIA
                     ECPAHIKTFVLQDFWGEVNFFFEKLADCYLCIDHHAAEITKKRFNAKTEVIGSPRHAI
                     YQTLNIEEQRNYIREYFNITQSTKTIGLFGQALHGIPGYMETISQWLQACLQQEKTKL
                     VIFYRPHPRESEADKEETIRLLNASGLEWKILSTKLVEESLLACDLVCSFFSNCLYDA
                     AYLNYFSLQPTLIPVVLNFNSQIQNYTNQLSLYEASPYRKQELAFFCESAHTLANDIA
                     HLLSKECSYKYWLQSKITLENPSKSIINTLTSLFNRIHNIA"
BASE COUNT         1697 a          811 c          761 g         1515 t
ORIGIN      
        1 ttaaaatgtc cgcatctcca ccttggctgt tagtatcata aatataaaag aaattatttt
       61 ttgtacataa aatcttgtat tgttccgttt caggaagata atacgggcaa taaataataa
      121 aacaaaattt tttatcttct tttaatttac aaaaaagctc ataaaaagca aaaaattgag
      181 cagcttcata aataatagct aaaataacct tatcagacat acaaaacctt agagaaaatg
      241 attggtttta tcatatatca aaagcaaaaa atctcttatt aaatttatta aaatctacaa
      301 caacctctat ttttacagca tcatataatc tgattaatca tactgacaag catccaggtg
      361 tagaatttag tgacattacc cataatttct gctaaaaagc aatttcatta ttattacaaa
      421 taaatcagta taataaagga tagatttatt atataaaaac ggaggttatt atttatgtca
      481 actgtcgcgc aatacaagaa ctactatcat tatatttccg acggctggag caaagaacct
      541 aaagaaatgt tcaagtttct tgctcaaaaa attaaagaat tgccattgaa acctggaaac
      601 aaattattag atattggctg tgcaacagga gaattaattc attttttaag acatcaattt
      661 ccacagctgc actataccgg aatagatgtt tttgatgact tgattgaggt atgcaaagaa
      721 cttcaaccga atgtaaattt tattaatgca tcagccttgg atctgccttt tgaatttgaa
      781 aatcaatttg atattattac tgttgtaagt gttatttcta tcttcgatac tatcgactta
      841 gatatattct gggataatct ctttaaagca accaaagata acggagtaat ctatgtcttg
      901 tcaccattta atgaatatgg ggtagattgt gaaatacgac atagaaaaag aataaataat
      961 attaaagggg aatgggaacg cggttggaat attttttgca aagaaacgat agcagaatat
     1021 cttgagggtc gttgtaaaaa ttggtttttt catcctttca aatttaatct tgaattgaat
     1081 cagcgtgaag acgtaattag aacctggaca atgaaaaccg agtttaatga taggcaatta
     1141 actaatggta tgaaattaat ggtagatcat tatttattag aaattcacat ataaacgaga
     1201 cagtcaatga gtcagccttt tacaattgga ccattctcta ttggagaaaa tcatcctcct
     1261 atttttttag cagaaatagg tacttttttt aatcaggatc ttggcatagc tgaacaattg
     1321 ctacacaaaa ttataaccat cagagataaa gtaccgtatc aacctcttat actaaaaaca
     1381 gaaattttaa ataacccaaa catttgccta cctgttgatt atgaagaaac ctacactagt
     1441 aaatcaggtg aggttcgcaa ggaaaactac cgctcattaa ttgagagaaa aatactgcct
     1501 ttagaacatt acagtatgct attctcacta ttaaagaatg ccaatttacc cttcatagtt
     1561 tcaatttatg aattggacac tattgatttt gcaataaagc aaggggcctg cgcactcaaa
     1621 acctcttcat caaacattgt ccatattcct ttaattcgtc atctggcaaa aacaggatta
     1681 ccgctcatta ttgacactgg aagatcttcc ttaactgata tatgcaaagc agttgaaaca
     1741 gcaaaaaatg ccggatgtga aaaaattatt attgaacata gtcctgatgg ccatcctgct
     1801 ttgccagaag cacataactt aagaatgtta cgaactttaa accaatgttt ttcattacca
     1861 gttggattat ctgaccatta taatggaatt gaaatgttgt atatgtctat tgcattggga
     1921 gcttcaatcc tggaaaaagg ggttcatctc tatccagaag atattgatca ggacatatca
     1981 catactatgg ggattgatca attagccaag actcttgaaa caatttacaa ttgttggcaa
     2041 gctttaggtc aaccaatgcg agatctggaa acccctataa aaggagtact tggcagctct
     2101 caaagaactt gtttggttgc aaagcaaaag cttcaacccg gctctctaat tagccttgat
     2161 aatacaacct ttgcctttcc ttgtaaggga gttcctaccc aatactggga tctggtaaat
     2221 ggctgggaac tacaaaacaa ggtatctata ggtgagccca ttaaatggtc agatattaag
     2281 caaaaaaaat gattgttaga aaccctactt ggaactctct tacccctccg cttcctaaat
     2341 ttgtttcttg taatacagtt gagacagatt tcctgaaaga aaaattaggc gaagatggta
     2401 gggtttatat tgctcaattt gatctaaaat caacttctgc ttttttattt cagcgtaaaa
     2461 gtgatcaaaa aaaatttttt atcaagaaag tatcctctga acataaaagg cagtatcagc
     2521 agagcgaaca tttggctcaa tttatcgctt gcccagatta tatagttaat attgcaatta
     2581 actgcgtttc taatgaagaa gaaaactcac tctattatat ttatccctat attcatggaa
     2641 aacgcctctt tgctgaacca gaggaaataa taagccttgc aactgcgtta gcaaaattac
     2701 atcttaaaaa aaaatcttat cctgaccaac aattgattat taaaaatacc acggaacgaa
     2761 catcacagct taattttata agaaaggctt tagctgatgg atgttattct tacattccac
     2821 atttctcttt tgtcaaaaag atggctcaac aatatgattt tgattggatt aatcaagaag
     2881 atgcccaacc aattcatggt gatttaaatg caggaaatct gttactatcc gaagataata
     2941 tgatttgctt ttttgacttt gaagacgcat tacatagttt ccatccagta gtgttagatt
     3001 tgctattcgt cattgagcgc attattttta atcaaaatag ttcgactgaa cagctattaa
     3061 accttggcct catgtttatt catgcatata aaaaggctgg tggcacatac caatataaaa
     3121 caagagatga atttggatta acaattcttg ctttaaaagc attttgtttg ttgactttgc
     3181 tagcagagaa aaataaaaat atactgagtt cggaatggga taaattttta aaattaaccc
     3241 aaaaagcaga aaatgaacgg ggtttgataa aaacaatttt acaaggataa gaaagatagt
     3301 gtgagaattc ttgtgactgc gcgggatgtt ggtgctgcgc taaacattat tgagatagta
     3361 aaaatactta agcaatatgc aggtgttact gtatttattt atgctcaacc ccctgcttca
     3421 aaatattttc ttcgggctgg gattcaacct gtatttgaag ttccactacc accaagccga
     3481 tccttaaatg aagccagtga cctactatcc tacgccaatc aattaattga taaattaaat
     3541 cccgatatca tattatctgg attatcatct cctggcgaag ctggcataga tgaagcaata
     3601 atagctgagt gtcctgcgca tatcaaaaca ttcgtccttc aagatttttg gggagaagtt
     3661 aattttttct ttgaaaaatt agctgattgc tatttatgca tagatcatca tgctgcagaa
     3721 attacaaaaa aacgatttaa tgccaaaacc gaggtgatag gctcccctcg ccatgccatt
     3781 tatcaaacat taaatattga ggaacaacgc aactatataa gagaatattt taacattact
     3841 caaagtacaa aaactattgg tctttttggg caagcacttc atggtatacc tggatatatg
     3901 gaaaccattt cacaatggtt gcaagcttgc ttacaacaag aaaaaaccaa attagttatt
     3961 ttttatcgcc cccatcccag agaatcagaa gctgacaaag aagaaactat aagattactt
     4021 aatgcatctg ggttagagtg gaaaattttg tcgactaaat tagttgaaga atcattgtta
     4081 gcatgcgact tggtttgtag ttttttttct aattgccttt acgatgcagc ctatttaaac
     4141 tacttttctt tacaacctac actgatccct gttgttctta atttcaactc acagattcaa
     4201 aattacacaa atcaattgag cttatatgaa gcttctccat atcgaaagca agagttagct
     4261 tttttttgtg aatctgctca tacactagct aatgacatag ctcatttatt atcaaaagaa
     4321 tgttcttata aatattggct gcaaagtaaa attacattag aaaatccatc taaatctata
     4381 attaatacat taaccagtct ttttaatcgc attcataata ttgcataaag ctaacagtaa
     4441 tcgctcactc cccttgtcat gtaagtatat aaaattatga tatttttacg acaagatgtt
     4501 tttattacca tcaaaaatcg ctataaggag tcaaaagata agactctgat tttatgactg
     4561 atttatccca aatcaattta actccgaaga atctagaaga agcattagaa acaataggcc
     4621 atttagcaaa aataatcatt gagttaaaag aagaaaatgc tcgattaaga ggcaccaaat
     4681 caatatgagt cgcagatatt aaaaacagta gattaattgc atgtctcttt tttcttgtct
     4741 gccggacctt tggaataaaa aaattgtaac ttcaaaagcc atcc
//
LOCUS       DASLOE010000195         4766 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.196, whole genome shotgun sequence.
ACCESSION   DASLOE010000195 DASLOE010000000
VERSION     DASLOE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.196"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            135..1559
                     /gene="sidA"
                     /locus_tag="U6309_002644"
     CDS             135..1559
                     /gene="sidA"
                     /locus_tag="U6309_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS effector SidA"
                     /protein_id="HEN5506060.1"
                     /translation="MAKKYRTPIPLSDKMMTLKKNLQDQLTQSQSLGEQIHAGTTAAS
                     TITTGVSHVFNVIDSFLSAYRQIPLVGGILQLVATVAKSISIVSDPEKSIAEKIISSI
                     LIAAIIALSITAITLGGLAAAIIGTVIASTVTIMEGLGFFGKIAEKFQLANSYKMKKE
                     FIDLVAKRIEPDNDKYNDLLEIRAIELQHELEKSYPNQIEQQQLKEELNFINTVLRKK
                     NITPGNNKENDAYKLNQLYAKRAEQLKTLVDKIALINPTGKRAEQNKLLSEIKSLQKE
                     ITKTDDSIEEIIAPLAKIERDNLKSTINLPLSYSTFAIAAASTLISIAGLLVVAGVIA
                     APPIMVPIMFAVGSVMAIIGLIKWTAEKMAEIEDAQLKAEETAKHEDAVLDEALNVYE
                     QQLNLELVSPANPARQVKNILTVQDKVPALSPTESTVSSLDSRFTLFGSSSREHTKIA
                     EQKTNDTTNEQAPTTFDSISKPTR"
     gene            1670..2623
                     /locus_tag="U6309_002645"
     CDS             1670..2623
                     /locus_tag="U6309_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 family protein"
                     /protein_id="HEN5506061.1"
                     /translation="MARNKRLDRFHNAQNSNTCGYDQAFSEIQGGRKRSHWIWYIFPQ
                     LENLGKSPSAKLYGIKDFNEACDYLKDPVLFKRYFELIKLIEEKLKNIPLPHLMGGFP
                     DDRKFISSLTLFQATSNYLSSKDTDSQNKYHELYETCNRVFKQIAPQGYSPCQQTELL
                     VKHQSNSPVKKTTEKSSQPPQVTIFSPKRSKPNTAHPILKDLAQYINKRGNEWSYHYN
                     FLGLVAFVYFIQDLILGTDYLNDKARQVKLTAASKLQKILDPQCHERCNLNPSERKAL
                     SDGRLGEIINKHGGLDKILTEAPRKPPKTGHDQEINISPFS"
     gene            2896..3468
                     /locus_tag="U6309_002646"
     CDS             2896..3468
                     /locus_tag="U6309_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEN5506062.1"
                     /translation="MSQNIILNRCEWVGQNKPHYELYHDTEWGIPVHDDEKHFEMLIL
                     EGAQAGLNWETILKKRDGYRRAFKQFNPQAVAQMTDDELNALLINPEIIRNRLKIFST
                     RKNAKVFLSIQQEYGSFDSYVWQFVNGAPILNRPENIRAIPATSKESDALSKDLKKRG
                     MSFVGSTIIYAYMQAIGMVNDHIVTCFRAK"
     gene            complement(3628..4719)
                     /locus_tag="U6309_002647"
     CDS             complement(3628..4719)
                     /locus_tag="U6309_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506063.1"
                     /translation="MKKYHQLLMGISLLLFGVCNYAGDTGPVENSDLFRVFIPNLKPG
                     YEFSATAFYLQPGADNLGWGVITTVLPIPSPNWQVVTFNPDYQVGFNLGGRYVFANSG
                     SDLQLNWSHLRTNDTDNVFVNPSSQWISPFSQTGTPPTPSGQITGVALLKLAHASLDF
                     SYDAVSLDVGKFINFGSKLQTRLFTGLSSAWIKEELISTFRGFSLPILSLNNTSTYKG
                     VGPRLGFNNSYNISHGFNLVGQLAGSVLYGRMQPAQYQFTGTSQQLIIAGIFVNREGL
                     ANPSVNQLVPALDAKIGLSYLYSLKQNWDLNFEAGYMGALYFNPLSSYETNTNVIALD
                     TGSLSTSSAKHTQSNFSVGGPYITVSLRT"
BASE COUNT         1663 a          932 c          841 g         1330 t
ORIGIN      
        1 tagtatacca agctcttctt tgttagtttt tgtttaaatt ccaaacaaat atcttcccaa
       61 aaggaaaatt aacaagtaaa attatgtact aaaataattc atatagttta ctaagtgtct
      121 ttagggttct ttttatggcc aaaaaatatc gaacgcccat tccattgtca gacaaaatga
      181 tgactcttaa aaagaatctt caggatcaac tgacacaatc ccaatcgtta ggtgagcaaa
      241 ttcatgctgg aactacggca gcatcaacaa taacaacggg agttagtcat gtgtttaatg
      301 tcattgactc ctttttaagc gcctatcgac aaattccact ggtaggcgga atactgcaac
      361 tagtggccac agttgccaaa tcaatctcaa ttgtgagcga cccggaaaaa tctattgctg
      421 aaaaaataat atcgtcgatt ctaattgctg ctattatcgc cctttctatt acagcgatca
      481 cgctaggtgg gctggctgcc gcaatcatag ggacagtgat tgcctctact gtaaccatca
      541 tggaaggatt aggatttttt gggaaaattg ctgaaaaatt tcaattggca aattcctaca
      601 aaatgaaaaa agaatttata gatcttgtag cgaaacgtat agagccagac aatgataaat
      661 ataatgactt attagaaatt cgtgccatag aactccaaca tgaacttgag aaatcttatc
      721 ccaatcaaat tgaacagcaa caattaaaag aagagttgaa tttcattaat acggtactta
      781 ggaaaaaaaa tatcaccccg ggaaacaaca aagagaatga cgcttacaaa ctaaaccaac
      841 tctatgctaa acgtgctgag caattaaaaa cccttgtaga taaaattgct ttaatcaatc
      901 caactggaaa gagagctgag caaaacaaac ttttaagcga aatcaaatca ttacaaaaag
      961 aaataaccaa aaccgacgat agcatagaag aaatcatagc cccgcttgct aaaatagaac
     1021 gagacaattt gaaatctacc attaatttgc cattaagtta cagcaccttt gctatagcag
     1081 cggccagtac tttaatatcg atagccggct tattggtggt ggctggcgtg attgccgcac
     1141 ctccgattat ggtccctatc atgtttgcag taggtagtgt tatggcaatc attggtttaa
     1201 tcaagtggac cgctgaaaaa atggctgaaa tagaggatgc tcaattaaaa gcagaagaaa
     1261 ctgcgaaaca cgaggatgca gttttggatg aagcattaaa cgtttatgag caacaattaa
     1321 atcttgagtt agtcagccct gcaaaccctg cgagacaggt gaaaaatata ttaacagttc
     1381 aagataaagt gcctgctttg tcgccaactg aaagtactgt aagctcatta gattccagat
     1441 ttactttatt tggctcatct tctcgagagc acacaaaaat agcagaacag aaaaccaatg
     1501 atacaactaa tgaacaagca cctacaactt ttgattccat atccaaacca actcgttaac
     1561 aaatataaca actttttata attttgttat aattgattta taaatagtta gtttatgcat
     1621 aatacctgat tcgtgaatat aaaaattaat gaaatcaaga gaaaaattca tggccagaaa
     1681 taaaagactc gatcgttttc ataatgctca aaatagtaat acttgtggct acgatcaagc
     1741 attcagcgaa atacaaggcg gaagaaaaag aagccattgg atatggtata tttttcctca
     1801 attggaaaat ttaggtaaaa gcccatcggc caaactatat ggaattaaag attttaatga
     1861 ggcatgtgat tatttaaaag atcctgtact gtttaagcgc tattttgagt taataaaact
     1921 catagaggaa aaattaaaga acattcctct acctcatctg atgggtggtt ttcctgatga
     1981 tcgtaaattc atctcaagtt tgaccttgtt ccaagccact tccaattacc tgagttcaaa
     2041 agatacggat tctcaaaaca agtatcatga gctttatgag acttgtaata gagtttttaa
     2101 acaaattgct ccacagggtt attctccatg tcaacaaact gagttattgg tcaaacatca
     2161 atcaaattcc cctgtgaaaa agacaacaga aaaatcaagt caacctcccc aagtgactat
     2221 cttttcaccc aaacggtcca aaccgaatac tgctcaccct atccttaagg atcttgctca
     2281 atatataaac aagaggggaa atgaatggag ttatcattac aattttctag gcctagtggc
     2341 tttcgtttat ttcatacagg atttaattct tggaaccgat tatttgaatg ataaagctcg
     2401 tcaagtcaaa ttaactgcgg caagcaaatt acagaaaatt cttgatcccc aatgccatga
     2461 acgatgtaat ctaaatccat cagaaagaaa ggcgctttcc gatggtcgct tgggggagat
     2521 tattaataaa cacgggggat tagataaaat cctgacagag gctcctagga aacctccaaa
     2581 aactggccac gatcaggaaa tcaatattag tcccttctca taaaaccatt ggatttcaag
     2641 tctttgcacc aagaaacatc acattataaa cccaatggct tttacaattt tttaatctct
     2701 acgaattcac tacaaaccac atcaattatt attcaaaaga ctcccataga gcgacatatg
     2761 caccagtcgg atcgactatc attacttgac aacgaataca acttaatgaa tagttcacac
     2821 agaatagtag accaatgtta taaatactgc cattaccagg attaaatatt tttaacccaa
     2881 tctaataatt acattatgag tcaaaacatc attctaaacc gatgcgaatg ggtagggcaa
     2941 aataaacccc attatgaact ataccatgat actgaatggg gaattcctgt tcatgatgat
     3001 gaaaaacatt tcgagatgct cattctggaa ggtgctcagg cagggttaaa ttgggaaact
     3061 atattaaaaa agcgcgatgg ataccggaga gctttcaagc aatttaaccc acaagccgta
     3121 gcccaaatga ctgatgatga attgaatgct ttgttaatta atcctgaaat tatccgcaac
     3181 cggcttaaaa tcttttctac cagaaaaaat gcgaaggttt ttctatctat tcaacaagaa
     3241 tatggatcgt ttgattctta cgtttggcaa tttgttaatg gggcacctat cttaaatcgc
     3301 cctgaaaaca tacgagcgat accagcaacc agcaaagaat cagatgcctt atccaaagat
     3361 ctgaaaaaaa gaggaatgag ctttgttggc tcaacaatca tctacgctta catgcaagcc
     3421 attggtatgg tgaatgatca catcgtaacc tgttttcgag ccaaataaag aaatcactcg
     3481 aactcctgaa ggtgactcat attctccata agctttttag cgaaatcatt taaaccggat
     3541 tatctgtcgt atcttggcct aaccataaat tggaaattca gttttcatat tttgttaggc
     3601 caaggaatga tagtttgacc aacctaatca ggttctcaag ctgactgtaa tgtaagggcc
     3661 acctacagaa aaattacttt gtgtgtgttt ggcacttgat gtcgacaaag agccagtgtc
     3721 caaggcaata acattggtat ttgtttcata actggataaa ggattaaaat acaaagctcc
     3781 catataccct gcttcaaaat tcaaatccca attttgtttc aatgagtaaa gataacttaa
     3841 cccaattttg gcatccagag ctggaaccag ttgattgaca ctgggattag caagtccttc
     3901 acgatttaca aagattccag cgataatcaa ttgctgagag gttcctgtga attggtattg
     3961 agctggttgc attctgccgt acaagactga gccggcgagt tgtccgacca aattaaaccc
     4021 atgagaaata ttataactat tattgaatcc cagtctcggc ccaacacctt tgtaagtgga
     4081 tgtattattt aatgacagta tagggagtga aaagccccga aaagttgata tcaactcttc
     4141 ctttatccag gcactgctta accctgtaaa aagccttgtc tgtaatttgg aaccaaagtt
     4201 aataaatttt ccaacatcga gactgactgc atcataacta aaatcaagac tggcatgtgc
     4261 aagcttcaat aaagccactc ctgtaatttg gccagaaggc gtaggaggag taccggtctg
     4321 agaaaaagga gagatccatt gcgatgaggg attcacaaag acattatcag tatcattggt
     4381 acgcagatga gaccagttta attgcaaatc gcttccagaa ttggcaaata cataacgtcc
     4441 tcccaaatta aacccaactt gataatctgg attaaaagta acaacctgcc aattaggcga
     4501 tggtattggc agtactgtcg taatcacccc ccaacctaaa ttatccgctc ccggttgcag
     4561 ataaaaagct gtcgcagaaa attcatatcc aggtttcaaa ttaggaatga agactcggaa
     4621 caggtcgcta ttttcaactg gccctgtatc accggcataa ttgcatacac caaacaacag
     4681 taagctaatt cccataagta attgatgata tttcttcata tttaatgaat cctccctaat
     4741 gcatgatttc tatcaatcag gactta
//
LOCUS       DASLOE010000196         4742 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.197, whole genome shotgun sequence.
ACCESSION   DASLOE010000196 DASLOE010000000
VERSION     DASLOE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.197"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(40..516)
                     /locus_tag="U6309_002648"
     CDS             complement(40..516)
                     /locus_tag="U6309_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter suffix domain-containing protein"
                     /protein_id="HEN5506064.1"
                     /translation="MKFRFRFYLGIILLILAIVCPLLIPLIVQSNFSVLIKGFLSSIL
                     VFGLPEVLIVLAIALLGKEVYGLLEQKIKNILFKEKVSRARYRLGLVFFAFPLVIGLL
                     EVFVKEITLAYGSYYYWVEIIWTTMFALSFFICGKQFWDKFKSLFIYDSYVVFEKN"
     gene            772..2295
                     /locus_tag="U6309_002649"
     CDS             772..2295
                     /locus_tag="U6309_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEN5506065.1"
                     /translation="MIMAATIPHIETITFTDWFLAIIDTPYIPIDEIEARAAEGFKRK
                     LTERQFQQLTQLIHLISFIKHRRLSNPTLALRIYVDENTSSLSRTALVEAVRMLPPED
                     NKTYELVAYLAQKNKLERYTNITKVPPLQIYQQNGVFEQCDEWILLFELFELTRATPN
                     DLIPVIADLLISRELGIPDLRALSKFMKTMDKLGVLSLSFMQKITPNLCNGQIIEKYE
                     SFLLKLQINDLLNKEILETIYSLIKELDALDAFFSLYHEELSHDSTLSFLREILPSFC
                     VMSRPSKESYDDQVSTNTPLHLAVIESDKANLERTLAFANRNLLIKSSYENTALLLAC
                     KLADKESARLILAKMQELGCDVNQKDHHGMTALHWANFYHFDELIRELEVAGADPKLK
                     AANGKTCEYFYQHQFTMNDLKLNGKEIIDGEFKLSECALTDIAFHMDKIALNLKLTTP
                     SEIAELYARDEIAQIRSSNRFYLFFKLFRPKLINWLEKQNDLEPQVTYRFSGQSSSA"
     gene            2456..3199
                     /locus_tag="U6309_002650"
     CDS             2456..3199
                     /locus_tag="U6309_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN5506066.1"
                     /translation="MVRLRIYLCSLLLVIPFYKPYALSLVIGTLSYNPPFETQVNDSS
                     KKNEFYGFEIELMDEICERINAKCQYKPLLFEQILAEINSGAIDLGIGSINITPERSE
                     RFLFSLPYKASYHQFLIPSNSNIKKIDDLKGKTIGIHLGSPTIDYLKAYFNNNIQFKY
                     YETFMDLLNGLTDPQVSAIVTNSDQAQYWASSTPDCKLLGDSFPFGIGFGVVARLDQA
                     ELIEKINQALIEIENDGTYLKIYNLFFQP"
     gene            3342..4064
                     /locus_tag="U6309_002651"
     CDS             3342..4064
                     /locus_tag="U6309_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HEN5506067.1"
                     /translation="MNYQYSPNLKKKLLLAFTILFIVQIFYFASVRASTRDTQLWTLM
                     NAIGPINKQHRKIRYWLEIQDRVGENISQLSQLLLRPGLGYELLPTTTIWLGYDRVYT
                     AQPFANPPVNENRVWQQLLWSKEYTRLRLTARSRLEERFIQRAIHTAWRYRQLFKTSI
                     FIPKHPKYAAVISDEAFFHLNDFNLQRNQGFDQNRLFVGLGYRTSKNSTIEIGYLNQM
                     IKRVNNPDYRGDYLSMALLLTE"
     gene            complement(4127..4483)
                     /locus_tag="U6309_002652"
     CDS             complement(4127..4483)
                     /locus_tag="U6309_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506068.1"
                     /translation="MSKFFTLLICISIGLLSFPTYAQKTIRLSPNETKTLKNSTLWTL
                     NATCSVQSSTQVNGKIKIIVLKNNGKVNGKSLSTGQGTSITIKNNSSISVSAESGTQI
                     NLINLGNQELQAVCST"
BASE COUNT         1608 a          867 c          786 g         1481 t
ORIGIN      
        1 gactaaacta gaaacaattc gaatgaatca acttattttt taattttttt caaaaacaac
       61 ataagaatca taaataaata aagatttaaa tttatcccaa aattgtttcc cgcaaataaa
      121 aaagcttaaa gcaaacatcg ttgtccaaat aatttctacc caataataat aacttccata
      181 ggctaaggta atttctttta caaacacctc cagcaaccct ataacaagtg gaaaagcaaa
      241 gaataccaaa cccagacgat aacgggctct tgatactttt tccttaaaaa ggatattttt
      301 aattttttgc tcgagaaggc catacacttc ttttccaagt aaagcgatag ctaagacaat
      361 caaaacttcc ggaagaccga atactaatat tgaagataga aatcccttta tcagcacaga
      421 gaaattggat tgcacaatta acgggatcag caaagggcaa actatcgcca aaattaaaag
      481 aataataccc agataaaaac ggaaccgaaa tttcaagatg gccactcaca ctatttacct
      541 tacttatcaa tagtaatcca gaagaagatt gaatatctac aaaatcaacc attaattcct
      601 gcaattatta aataaagcct cgctcatctg gtttttccta taaccaccgg atgccatgaa
      661 actgaactcc atgaccttaa agatctttta tcatatttat tttattaaat gtcattttgt
      721 tgttatcaaa acaatacttt ttgtttataa tattgacaat ttatttaagg aatgattatg
      781 gctgcaacaa ttccccatat tgaaacaatc acatttaccg actggttcct cgcaataatt
      841 gacactccgt acattcccat cgatgaaatt gaagcaaggg cggccgaagg ctttaagaga
      901 aaattgacag aacggcaatt tcaacaatta actcaattaa tccatttaat cagtttcatt
      961 aagcacaggc gtttatccaa tcctaccttg gctctccgca tctatgttga tgaaaatacg
     1021 agctctctat caagaactgc tttggttgaa gcagtcagga tgcttccacc tgaagataat
     1081 aaaacctatg agttggtagc ctacttggct caaaaaaaca aactggaacg atatactaac
     1141 ataacgaaag tacctcctct gcaaatttac cagcagaatg gcgtatttga gcaatgtgat
     1201 gaatggattc tgctttttga attgttcgag ctaaccaggg caacacccaa tgatttaatt
     1261 ccagtaattg ctgatttatt aattagcagg gagcttggta tccctgatct tcgggcactt
     1321 tcaaaattta tgaaaactat ggataaatta ggcgtattaa gcctaagttt tatgcaaaaa
     1381 atcactccta atttgtgtaa tgggcaaatt atagaaaaat acgagtcatt cttacttaaa
     1441 ttacaaatta acgatttgtt aaacaaggaa attctggaaa caatttattc actgatcaaa
     1501 gaactagatg ctcttgacgc ttttttttca ctctatcatg aagaactatc gcacgacagt
     1561 actctgtctt tcctgagaga aattttgcca tccttttgtg taatgagccg ccccagtaaa
     1621 gaaagttatg acgatcaggt atcaacaaat acgcctttgc atctggctgt aattgagagt
     1681 gataaagcga atctggaacg aaccttagcc tttgccaatc gcaatttact gataaaatcc
     1741 tcttatgaaa atacagcact tttattggct tgcaagcttg ctgataaaga gtcagcccga
     1801 ttgattttag cgaaaatgca agaattaggc tgtgatgtca atcaaaaaga tcatcatggt
     1861 atgaccgcct tgcattgggc taatttttat cattttgatg agctaatccg ggaattggag
     1921 gttgctgggg cggatcccaa attaaaagca gccaatggaa aaacctgtga gtatttctat
     1981 caacatcaat tcactatgaa cgatttaaaa ttaaatggca aagaaatcat tgatggtgaa
     2041 ttcaagttaa gtgaatgtgc gctgacagat attgctttcc atatggataa aatagcctta
     2101 aatttaaaat taacaacacc atccgagatt gcagaactgt acgcaaggga tgaaatagca
     2161 caaattcgtt caagcaaccg gttctatttg ttttttaaat tattccggcc aaaattaatc
     2221 aattggttag agaagcaaaa tgacctggag cctcaagtga cttatcgttt ctcagggcaa
     2281 agttcttctg cttgatatca ttaacttaac aaacccggta caactggcaa tttacttgct
     2341 ctgttctata caaggtaaag cgccctgatt tatgaaaaaa tataaatcca accgttcttt
     2401 tatttttcaa aaataaggct aaaataataa ataactatat aaaaaggata tttaaatggt
     2461 tcgcctccga atttatttat gttctttgct actggttatt cccttttata aaccgtatgc
     2521 gctgagtttg gtcataggta ccttatctta caatcctccg tttgaaactc aagtcaacga
     2581 ttcttccaag aaaaatgagt tttatggttt tgaaattgaa ctcatggatg agatttgcga
     2641 gcgcatcaat gcaaaatgcc aatacaagcc attgttattt gaacaaattt tagctgaaat
     2701 taattctggt gcaatcgatt taggcattgg tagcattaat attacgcctg aaagaagcga
     2761 acgattttta ttcagccttc cctacaaagc cagctatcac cagtttctta taccatctaa
     2821 ctcgaatata aaaaaaattg acgatctcaa aggaaagact ataggaattc atttaggttc
     2881 acctactatc gattatttaa aggcctactt taacaataat attcaattta aatattacga
     2941 aacatttatg gatttactta atgggctgac tgatccgcaa gttagcgcaa tagtgactaa
     3001 tagcgatcaa gcgcaatact gggcatcgag tacaccggac tgtaagttat taggcgacag
     3061 ttttcctttt ggaataggat ttggagttgt cgcgagatta gatcaagcag aactgattga
     3121 aaaaatcaat caggcattaa ttgagattga aaatgatggg acttacctta aaatatataa
     3181 cctcttcttt caaccttaag tattatttcg acagacaaag gattcaagtg cttaataaat
     3241 ctattgattt ttgtctataa tctgagaaac aataaaataa aaaatattga cgatgacttc
     3301 tcaaacctga tgtggaataa ctttttaagt ctttttaaac aatgaattat caatattccc
     3361 ccaatctaaa gaaaaaactc ctgctcgcat ttactatttt atttattgta caaatcttct
     3421 attttgcctc tgttagagca tcaacaaggg atactcaatt atggacgctt atgaatgcga
     3481 taggaccaat caataagcag catcgcaaaa taagatattg gcttgagata caggatagag
     3541 ttggtgaaaa tatttcgcaa ttgtctcaac tgctattacg ccctggccta ggatatgaac
     3601 tattgcctac aacaaccatc tggctcggat atgatagagt ttacactgcg caaccatttg
     3661 ccaaccctcc tgtaaacgaa aatcgggttt ggcaacaatt attgtggagc aaggaatata
     3721 ctcgcttaag acttacggct cgttcgcgtt tggaagaacg ctttatccaa agagctattc
     3781 ataccgcttg gcggtacaga caattattta aaaccagtat ttttattccc aaacacccta
     3841 aatacgcagc cgtgattagt gatgaagctt tttttcatct gaatgacttc aatcttcaac
     3901 gtaatcaagg ctttgatcaa aaccgtttat ttgtaggcct aggttataga accagtaaaa
     3961 acagcacgat agagattggg tatctcaatc aaatgattaa acgcgtaaat aaccctgact
     4021 atcgcggaga ttatttatcc atggctctat tattaacaga ataaacaagt ggacaaagaa
     4081 aacaaaccat tactttcaat ggtttgtttg agaaaaattg atttaattaa gtagaacaaa
     4141 ctgcttgcaa ttcttgatta cccaagttaa tcagattaat ctgagtgcct gattctgcac
     4201 taaccgagat gctgctgtta ttttttattg taatagaagt tccctggccg gttgaaaggc
     4261 ttttaccatt tactttgcca ttgttcttta aaacaatgat ttttatttta ccattaacct
     4321 gcgtgctgct ctgtacgcta caggtcgcat tcaaagtcca taaagtgcta ttttttagcg
     4381 ttttggtttc gttagggcta agtcgtatcg ttttttgagc ataagttggg aagcttaata
     4441 aaccaattga gatacatatc aacaaagtaa aaaatttaga catgctactt tccttctcca
     4501 gaatccaagg cccaactatt ttaatgcaat atgtatatat tatatacaga tttacttaag
     4561 attttattaa gcccgtttgt aaattttttt gaagtatccc attcagcaat cagttctcat
     4621 ggataaattg atttaattgg tttgcaattt ttgctttttt agcaaaagaa gaatcatacg
     4681 ttgttactaa aggaataggt ggcttatcct ttagagcgat ggcaagtcta taagttttcg
     4741 ca
//
LOCUS       DASLOE010000197         4647 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.198, whole genome shotgun sequence.
ACCESSION   DASLOE010000197 DASLOE010000000
VERSION     DASLOE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.198"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1913
                     /locus_tag="U6309_002653"
     CDS             <1..1913
                     /locus_tag="U6309_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEN5506069.1"
                     /translation="IFDHLHINLVPAGFLGVDVFFVISGFLITQIIFRNTKTQTFSLT
                     KFYLNRMRRILPALMLVLLVSSVMSYFLLLAPDLKNYSTSLLATILSSSNIYFWKFIN
                     IGYFSTDATIQQLLHTWSLGVEEQFYLIWPIILIVLTKKFTYKKIILGTILLTIISFC
                     AYFLLRKHPLLAYYSPITRAFELLTGALVALNFDNIPAVSNKHLKNLVSILGLFLIIY
                     PACAFTNNDFPSIKILLPCLGTALLIYTGKNNQGFANKIIENRILVFTGLISYSLYLW
                     HWPLISYLNYLKISINFTIGFVLILILLLTSTFTYKFVEFPFRKKLQLSFLNTILLFF
                     ILPLALAIIFYLFSQYKDNFGFNKISPEKLNQVNTYYGLLKQDYGCIDNKDGFSVLPS
                     EKLCTIGDFHSNKKTVLVVGDSHATAYVGMLHVLLSDQHLQAYVVNQSGTPFILGNIS
                     NWRENNPMSRNELLRKLIKNKKYDYVVMGGFWDYYPDLPSLDGKKHPPFEVFKIGLRE
                     AVKFIVDNKSIPVIMFDNPPLINLSKTCGLTRISFLDCSNNLREVKKIQNATRKIILT
                     LKNEFPQLILIDPTKIICDKSKCYTSINGTPLYFDEGANSHLNYDGSTLIGKLYLKKF
                     GNPFQTKNSIYS"
     gene            complement(2039..2233)
                     /gene="csrA"
                     /locus_tag="U6309_002654"
     CDS             complement(2039..2233)
                     /gene="csrA"
                     /locus_tag="U6309_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEN5506070.1"
                     /translation="MLILTRRIGETLIIGDDVNITVLGVKGNQVRLGINAPKDVSVHR
                     EEIYLRIQQEKESDDSEQAV"
     gene            complement(2793..3500)
                     /locus_tag="U6309_002655"
     CDS             complement(2793..3500)
                     /locus_tag="U6309_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN5506071.1"
                     /translation="MIFHQLFDQDSCTYTYLIGSTFSKHAIIIDPVRSQVSHYINLIN
                     KLELNLVASIDTHLHADHITGSGQLTILTDCHSMIGMESKAKFVHIKFHDNEILNFGN
                     IKIKAMHTPGHTPDSYCFVMEDRIFTGDTLLINATGRTDFQNGSASAQYDSLFNKLLK
                     LPGFMRIYPGHDYNQKQYSTIDDEKKNNPRLQVKSRQEYVEMMGNLSLSFPKNMYFAV
                     PANLNNGLTEQEVNALV"
     gene            3732..>4647
                     /locus_tag="U6309_002656"
     CDS             3732..>4647
                     /locus_tag="U6309_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN5506072.1"
                     /translation="MIERAFSLLFIQFSIMQIQNTEINIGVIIVNYNSAHCLAKAITA
                     LEKQSYKPYQIILFDNSSIENLPEIVHTTKLPIKIVVSNINLGFAAGNNKAIEYLDPK
                     VNWIALLNPDAYPHKDWLLEMVSAIKTHPQYAFMGSKLICENEPYLLDGTGDVYHISG
                     KAWRLNHRKPLDKAPKDMQELFAPCAAAALYRRDAFNAVKGFDEKYFCYYEDVDLAFR
                     LWLAGYKGCYIPTAIAEHTGSAITKKHSDFYTYYGHRNLVWTYFKNMPLLLLIATLPL
                     HLLLNIATLLLFIKRKQTFVIIKSKIDAI"
BASE COUNT         1604 a          789 c          669 g         1585 t
ORIGIN      
        1 taatttttga tcatctacat atcaatttgg tacccgctgg ctttttaggg gttgacgtat
       61 tttttgtaat atccggtttt ttaatcacac agataatatt tagaaacaca aaaacccaaa
      121 cattctcatt aaccaaattt tacctaaacc gaatgcgaag aatccttcct gcattaatgc
      181 tagtccttct agtttcctct gttatgtcct attttctttt attagcccca gatctaaaaa
      241 attacagtac atcacttctg gcaactattc tctcaagctc aaatatctat ttttggaaat
      301 tcattaatat tggatatttc tcaacagatg ctaccattca acagttactt catacttggt
      361 ctctgggtgt agaggaacaa ttctatttaa tatggcccat catactaatt gtactaacta
      421 aaaaatttac ttataaaaaa attatattag gcacaatttt gttaacaatt atctcgtttt
      481 gcgcctattt tttgttaagg aaacatcctc ttttggcata ttattcacca attactcgag
      541 cattcgaatt attaacaggc gcattagtgg cattgaattt cgataatatt cccgcagtaa
      601 gcaataaaca tttaaaaaat ctggtttcta ttttaggcct ttttttgatt atctatcctg
      661 cttgcgcctt cactaataat gactttccta gcattaaaat ccttttgcct tgtttaggta
      721 cagcgctttt aatttacacg ggaaaaaata accaaggatt tgcaaacaaa attatagaaa
      781 acaggatatt agtctttact ggtttaattt catactcgct gtacttgtgg cattggcctt
      841 taatttcata tttaaattat ttaaaaatct caatcaattt cacaattgga tttgttctta
      901 tattgatttt gctattaact tcaaccttta cttacaaatt tgttgagttt ccgtttagaa
      961 aaaaattaca gctttccttt ctcaacacaa tactactttt ttttattctt cctcttgctc
     1021 ttgcaattat tttttactta ttttctcaat acaaagataa ttttggattt aataaaattt
     1081 ctccggaaaa acttaatcag gtaaatacct attatgggtt gttaaaacaa gactatggtt
     1141 gcattgataa taaagatgga ttttcagttt taccatcaga aaaattatgt acgataggag
     1201 atttccattc caataaaaaa actgttttgg tagttggtga cagtcatgca acagcatatg
     1261 ttggtatgtt gcatgtgtta ctgtccgatc aacatttaca agcttatgtg gtcaatcaat
     1321 caggaacccc ctttatatta ggaaacatat cgaattggag agaaaacaat cctatgagcc
     1381 ggaatgagct tctcagaaag ttaattaaaa ataaaaaata tgattatgtc gtaatgggcg
     1441 ggttttggga ttattaccct gatttgccat ccttggatgg caaaaaacat cctccttttg
     1501 aagtattcaa gataggatta cgtgaagcag taaaatttat tgttgataac aaaagtatcc
     1561 cggtaattat gtttgataat ccaccattga taaacctgag taaaacttgc ggattgacaa
     1621 ggatatcctt tctggactgc tccaataact tacgtgaggt aaaaaaaatt caaaatgcta
     1681 ccagaaaaat tattttgacc ttaaagaatg agttcccaca gcttatatta attgatccga
     1741 caaaaataat atgcgacaaa tcgaaatgct atacctcaat taacgggaca ccattatatt
     1801 tcgatgaagg tgcaaattct catttgaact atgatggttc gacacttatt ggaaaactat
     1861 atctcaaaaa atttggtaat ccatttcaaa caaaaaactc aatatatagt tagtattaat
     1921 tttgatttgc tgatgagtgt ataataagca ttattaaaga ggaagttaca tttagttaca
     1981 tagttcgtcc cgagtatttt taagtactcg gaactggatg aactgcgtgg aaatcgattt
     2041 atactgcttg ttccgaatca tcagattctt tttcttgttg tatgcgcaaa taaatttctt
     2101 cacgatgaac agaaacatct ttcggagcat taattcctaa gcgaacttga ttgcctttta
     2161 cacctaaaac agtaatattg acatcatcac ctatgattaa tgtttcacct atacgccgag
     2221 tcaaaatcaa catgactaaa atctccctaa catagcagct acctacttct ccatagaacg
     2281 cttccatggt gttcttggca actacaacat gaacaatatt taagcaaatt tattgcttgt
     2341 atcacattgt acactctgat tattaacgga atattaagtc atagataaat ttgcgtttaa
     2401 tttttgcaga aatgagttga aaaattaatt tactctttgt cataaattca tcgtaaaact
     2461 gttcaacatt ccctaatatc aaaaacttcc aattataaat aaaccagact caaaaggaaa
     2521 aacaagtttt ttccctgcaa taaattcaaa aaaaaaataa aaatacctat ttaaaatcat
     2581 atatatacga tattaataga ataaatttac aactaaactc gcaagattac tagtttatta
     2641 attgaaataa taaactggca tataaaattc tgttcctaaa attatctgtt attcaaatta
     2701 caaaagtatc ttgagcttat attgcaattt atctttataa atcccaaact aggtaaatga
     2761 ataaaatata ataaaataaa tgataataat ttttaaacta aagcattaac ttcctgctca
     2821 gttaatccat tattcaagtt ggcgggaact gcaaaataca tgtttttagg aaaagacaaa
     2881 ctcagattac ccatcatttc aacatactct tgcctggatt ttacctgcaa tctgggatta
     2941 ttttttttct catcatcaat tgtactatat tgtttttgat tataatcatg gccaggatat
     3001 attctcatga atcccggtaa ttttaaaagt ttgttaaaaa gactgtcata ttgcgcactg
     3061 gcgctaccat tttgaaaatc agtacgtcct gtagcattga ttaataaagt gtcgccagta
     3121 aatattctat cttccattac aaaacaatag gaatctggag tatgccctgg tgtgtgcata
     3181 gcctttattt taatatttcc aaaatttaaa atctcattat catgaaattt gatgtgcaca
     3241 aattttgctt tgctttccat tcctatcata gaatgacaat cagtaaggat agttaattgg
     3301 cctgatccag taatatgatc agcatgtaaa tgtgtatcaa ttgaagcaac caaatttaat
     3361 tctaacttgt taattagatt aatataatgc gaaacttgag atcgtaccgg atcaataata
     3421 attgcatgct tgctaaaagt agagcctatc aggtaagtat aagtgcagga atcctgatca
     3481 aatagctggt ggaaaatcat tttatcaatc cctataaaaa aactttttgt tattagccat
     3541 gtttaataac ttgaattaat tgagtagcat aaatcaaact aataaaaaaa tattttattt
     3601 tttcatctgc gcaaaaatga aaataaattc acaatatata gcaaaaagac tgcatatcca
     3661 ataaaaacaa agcattaatt taaatatcat taaagattaa aaaatgatat ccttggcata
     3721 aactggagta aatgatagaa cgcgcttttt ctttgctctt catccaattt tcaattatgc
     3781 aaattcaaaa tactgaaatt aatataggcg ttataattgt taattataat tcagcacatt
     3841 gcctcgctaa ggcaattact gctttagaga agcaatcata caaaccgtat caaataatac
     3901 tgtttgataa ttcgagcatt gaaaatttac ccgagatcgt ccacacaact aaattaccaa
     3961 ttaaaattgt tgtatccaat attaacctgg gatttgcagc tgggaacaat aaggccattg
     4021 aatatctaga tccaaaagtc aattggatag cattgcttaa tcctgacgcc tacccgcaca
     4081 aggactggtt actagaaatg gttagtgcaa ttaaaactca cccgcaatat gctttcatgg
     4141 ggtcaaaact aatttgtgaa aatgagcctt atctattaga tgggacaggc gatgtgtacc
     4201 atattagcgg aaaagcatgg agattaaacc acagaaaacc attagacaaa gcccccaaag
     4261 atatgcaaga actgtttgcc ccctgcgctg cagctgcctt gtatagacgt gatgcattca
     4321 atgctgtaaa agggttcgat gagaaatatt tttgttatta tgaagatgtt gatttggctt
     4381 ttcgattatg gctagcaggt tataaaggat gttatattcc tacagctatc gctgagcata
     4441 caggctccgc cattacaaag aaacatagtg atttttatac ttattatggc catagaaatc
     4501 tagtttggac ctatttcaag aatatgccat tgttattgct tattgcaact ttacccttac
     4561 acctcctttt aaatattgct actcttcttt tattcattaa acgaaagcaa acttttgtaa
     4621 ttataaaatc taaaattgat gcgatta
//
LOCUS       DASLOE010000198         4639 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.199, whole genome shotgun sequence.
ACCESSION   DASLOE010000198 DASLOE010000000
VERSION     DASLOE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.199"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            246..533
                     /locus_tag="U6309_002657"
     CDS             246..533
                     /locus_tag="U6309_002657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506073.1"
                     /translation="MEPLETNVTYFVIGKPITTIESNSKQRKKISDDELKEAVPTGHA
                     LLFRTREEADEYARLTEGYNSKVPDCPRYKDAVFEVQLRNTIDEPQVVTKK"
     gene            533..1162
                     /locus_tag="U6309_002658"
     CDS             533..1162
                     /locus_tag="U6309_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506074.1"
                     /translation="MSHYGQSEVQYVKYVKVDADNLRYISGGVPGIPGKYQFKPTDEV
                     NVKTPKEWLAKIGFDRKIEALQNKVTMLGKEDDRASGELSFIIDKLIKYKQEFLDGHK
                     SAEQFVEACRDEIKPENTKNLANHRGIFASVTSFIESIVQWVSPKYKINTDSINKINE
                     VEKSLGEIKPERVVKETGIAKRTIKSDYEVDSDNTVAKAGDRRPGIKPG"
     gene            1431..1808
                     /locus_tag="U6309_002659"
     CDS             1431..1808
                     /locus_tag="U6309_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEN5506075.1"
                     /translation="MRDPEISLLDHLPGYIGWKDLNRHYVGANKALLELKGFRHVEEL
                     AGKTDEELSPWAIEENKLFQQQDFHVINGEKVATVHFNAKTSEVFLLEKCSLTDQNNQ
                     VTGLIYHYHIPARLKQVAVIKSL"
     gene            1913..2896
                     /locus_tag="U6309_002660"
     CDS             1913..2896
                     /locus_tag="U6309_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEN5506076.1"
                     /translation="MRQLFIIMFVFLLSNVGYGAPAFEILPLGVYGGLKDGNLSAYLI
                     KAIESKNYTALDGGTLVRGLEVAVDKQSLSNKRVDDLLQKHIPAYLISHAHLDHVMGL
                     VMAQPELREKQTIMAREETMQALQKNIFNWSVWGNFGDSGDTPQLNFQHYQTVPLFQW
                     VSIPDTDLHVKVFPLSHGGMASSAFLIRYEAEYVLYFGDTGADSIEKSTNMENIWKEI
                     APLIRNRQLHAIMLECSFLNSQPDDKLFGHLKPNLFMSELRQLASIVDPFDSRNSLRG
                     LPVVVTHIKPRLADFSNTNEDTEKQIINELSQENDIGVELIKPEQGITLSL"
     gene            complement(3078..4082)
                     /locus_tag="U6309_002661"
     CDS             complement(3078..4082)
                     /locus_tag="U6309_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN5506077.1"
                     /translation="MKQTAIVLQGGGALGAYELGVLKYLYASDSFSPNIISGVSIGAI
                     NAATLIGAKDDPIQTLEAIWDELTVFSAQMFPENTEPYLSLFGNAAFYHLRTDYWSMP
                     YWTNFYTTTPLQQLLLKYIDFDKLNNSPIHLILTATDVTTGKSTVFENQGNNRTEITP
                     LHVLASGSLPPGFPMTLIGNTHYWDGGLFSNTPLSPVMEKLNPDPNVEKQIIVINLFP
                     SQGKIPTNMPEVFDRVFEIIFSNKIRFNTELTEKINEYIETIQEIEKVIPENSFIRQL
                     PGYKRLLNYKYIQDIIYIESSDEENATASFDFSRKAIQRRIDSGYKDAKNNFQLNQLI
                     "
BASE COUNT         1506 a          787 c          941 g         1405 t
ORIGIN      
        1 aaaatatttc aagatcctct ggttaataat atttttaagt gtttttattt taaccaatta
       61 atctggattt ttaattaaca gatatgtttg attttcgaca taatgcgggg tttttcattc
      121 agaattataa agtttatgtg ccctatccct acctctcgtg gagcacagag tttaggataa
      181 agaactttga gttggttatt ttttatacta aacttacact agataagttc actggaggct
      241 attgtatgga accgttagaa acgaatgtta cctatttcgt gataggtaaa ccaattacaa
      301 ccatagaatc taattccaaa caaagaaaaa aaatttctga tgatgaacta aaagaggctg
      361 tcccgacagg gcacgctttg ctgtttcgta cacgagagga agctgatgaa tatgcgagac
      421 ttacggaagg atataatagc aaagtaccgg actgtcctag gtacaaagac gctgtatttg
      481 aggttcagct taggaatact attgatgaac ctcaagtggt tacaaaaaaa taatgtcaca
      541 ttatgggcaa tctgaagttc aatatgtaaa gtatgtaaag gtagatgctg ataatttgcg
      601 ttatatttca ggcggagttc ctgggattcc gggtaaatat caatttaagc caacagatga
      661 ggtaaacgtt aaaactccaa aggaatggtt agctaaaatc gggtttgacc gcaagattga
      721 agctctgcag aataaagtga caatgctcgg aaaagaggat gaccgtgcaa gcggggagct
      781 gagtttcatt attgataaat taataaaata taaacaggaa tttcttgatg gtcataaatc
      841 agctgagcaa tttgtagagg cttgtagaga tgagataaaa cctgaaaata cgaaaaactt
      901 ggcaaatcac cgtgggattt ttgcctcagt cacatcattt attgaatcta ttgttcaatg
      961 ggtaagccca aaatacaaaa ttaatacaga ttccattaat aaaataaatg aagttgagaa
     1021 atcattaggt gaaatcaaac cagaacgagt tgttaaggaa acaggtatcg caaaaagaac
     1081 aattaaatct gactacgaag tagatagcga caatacagtg gctaaagctg gagataggag
     1141 accaggtatt aaaccagggt agtttacaga gtggtttcat atgaattggg ctttcataaa
     1201 ttcgacgtgc tgatagctat taaaaaagcc tgtggttaag agcccattat ttcaactcca
     1261 tcccagagtt tagaaatgac aataaaatgc ttacctttct ttaaagacaa cgattgatta
     1321 gactcggctc tgtgatataa gcagctgatt cttccaatct gccttgaaaa ttacgcccca
     1381 accgtttaat aatccatgct cgaaatgagc attcattgga ggatgggcta atgcgggatc
     1441 ctgaaatatc attacttgat catctcccag gctatattgg ctggaaggat ttaaaccgcc
     1501 attatgtagg cgcaaacaaa gcgttgctgg aattgaaagg atttcgtcat gtagaagagc
     1561 ttgcgggtaa aacagatgaa gagctgtcgc cgtgggctat tgaagagaat aaactgttcc
     1621 agcagcaaga ttttcacgta ataaacggtg aaaaagtagc aacggttcat tttaatgcaa
     1681 agactagtga agtgtttctg ttggaaaaat gttctttaac agatcagaat aatcaggtga
     1741 caggattgat ttatcattat catatcccag caagactaaa gcaagtggct gttataaagt
     1801 cactttaatc attatgatgt tgaacttcca ccgtataaat acggtgattg aagcaaggtt
     1861 gaataaggta ttattttaca aactgacccg aattatttga aaggaaatcc tcatgcgcca
     1921 gctattcatt atcatgtttg tttttctatt atctaatgta ggatatggtg cgcctgcctt
     1981 tgaaattctt ccccttggag tctatggtgg tttgaaagat ggaaatttat cagcttattt
     2041 aatcaaggcc atcgagagca aaaattatac ggctcttgat gggggcactt tggtgcgagg
     2101 gcttgaagtt gcagtcgata aacagtcact gtctaataaa agggttgacg atttgttgca
     2161 aaaacatata ccagcctacc ttatttctca tgcccatctt gatcatgtta tggggcttgt
     2221 gatggctcaa cccgaattgc gagaaaagca aaccattatg gcacgtgaag aaaccatgca
     2281 ggcattgcag aaaaatattt ttaattggtc tgtttggggg aattttggag atagtggtga
     2341 cacccctcaa ttgaattttc aacattacca aacggtgcca ttattccagt gggtttccat
     2401 tcctgacacg gatttgcacg ttaaagtgtt ccctctaagc catggaggaa tggcatcaag
     2461 cgctttttta atccgttatg aggctgaata cgtcctctat tttggtgata ctggtgctga
     2521 tagcattgaa aagtcaacca atatggagaa catttggaaa gaaattgccc cactcataag
     2581 aaataggcag ctgcatgcca tcatgttgga gtgctcattt ttaaattcac agccagacga
     2641 caaattattt ggtcatctaa aacctaacct attcatgtca gaactgcgac aattagcatc
     2701 gattgttgat cctttcgact caagaaactc actacgaggt ttacccgttg ttgttacaca
     2761 catcaaaccc agattagctg acttttcaaa tactaatgaa gatacagaaa aacagattat
     2821 aaacgaattg tcccaggaaa atgatattgg cgtcgaatta attaaacctg agcaaggtat
     2881 aaccttaagt ttatgattga tgaagagtca caacagctgc cagaaccaat cctaataaat
     2941 catttatggt atttgtgtat caggggctgt tgcgaaaaat ccgaaccaga ataaaaaaac
     3001 cttagcatag agaagttgct actcctgcaa ctttgcatgc tatgacggca gcaatgtaat
     3061 agtggatgca gcagagttca aattaactga ttcaactgaa aattattttt ggcatctttg
     3121 tatcctgagt caatgcgcct ttgtatcgct ttgcgggaaa aatcaaaaga agcagttgcg
     3181 ttttcttcat ctgagctttc tatataaatg atatcctgaa tatatttgta attaagcagt
     3241 cgtttatagc caggaagctg tcttataaag ctattttccg gaatcacttt ctcaatttcc
     3301 tgtattgttt caatgtactc attaattttt tcggttaatt cggtattaaa acggatttta
     3361 ttggaaaaaa tgatttcaaa cactctgtca aaaacttcag gcatattagt cggtattttt
     3421 ccctggctgg ggaaaagatt aatcacaata atttgctttt caacgtttgg atcgggattt
     3481 agtttttcca taactggaga tagtggagta ttggaaaata agccaccgtc ccaataatga
     3541 gtattaccaa tgagcgtcat gggaaagcca ggaggcaaac tgccacttgc taacacatgc
     3601 aaaggcgtga tttctgtgcg gttattaccc tgattctcaa aaacagtact tttacccgtt
     3661 gtaacatcag tcgctgtcag aattaaatgg attggactgt tattcagctt atcaaaatca
     3721 atgtatttta ataataattg ctgaagtggt gttgtggtat aaaaattggt ccaataaggc
     3781 atagaccagt aatcagtacg caaatgataa aaagcagcat tgccaaagag tgacagataa
     3841 ggttcggtat tttcaggaaa catctgggca gaaaaaacag tcaattcgtc ccatattgct
     3901 tccagagtct ggataggatc gtctttcgcc ccaatcagtg tcgctgcatt gatagcccca
     3961 atagaaacac ctgaaattat gtttggcgaa aaactgtctg atgcatagag gtattttaat
     4021 acgcctaact cataagcacc cagtgctcct cctccctgga gtacaatggc tgtttgcttc
     4081 atggatgtcc ttatcatttc accctgtatg agctagtata gcagttaatt ataatgaaaa
     4141 ggatgtgatg caagtcctgg attgtcagaa gcaaggtata ttgccatcca gttaaatcag
     4201 cgctgacttt gatagggtgg ggcgcaaaaa ttggccgcaa acgattttaa gcatttgtta
     4261 agcattttga ctttcctgga aaaaatatta ttatgaataa ttctattagg gattaaatgg
     4321 cctgtttttt tatagcggcg atagctccag acatacaaag ccttgcttac gcgatgtgta
     4381 ttattgtaat gaaccggagt aatcagataa atgctctcta aagcttgatg atacctacat
     4441 ctgaataaga ttattcttat aaaacaggaa gatttgatgg aagttgacca tattaaaaat
     4501 gaagtaaaat tatttttact caactctttt cgaattaagg atattggtta ttcggataat
     4561 atttttgata caggcgcaat gcactcttta tttttcattc aattattagt ttttatagag
     4621 aagaaatata aaattgatc
//
LOCUS       DASLOE010000199         4602 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.200, whole genome shotgun sequence.
ACCESSION   DASLOE010000199 DASLOE010000000
VERSION     DASLOE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.200"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            15..167
                     /locus_tag="U6309_002662"
     CDS             15..167
                     /locus_tag="U6309_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506078.1"
                     /translation="MFNLKRNLIKLGTLSISIALLAACHTVKGTVTGAKQDIKATGKA
                     ISRTMQ"
     gene            complement(228..1094)
                     /locus_tag="U6309_002663"
     CDS             complement(228..1094)
                     /locus_tag="U6309_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEN5506079.1"
                     /translation="MLDGIMVLWFILLMLSLAFIAYDVREMPIDWVQKLGWVLVVAYT
                     GPLGLFFYFLTCRSPGKGLHALYTKAQWKQAVNSEVHCLAGDATGIIFAAIFLSFIDF
                     SNGIELILEYISAFFFGWVIFQAGMMRSMYSSYGEALRKTFFAETISMNFVMMGMIPV
                     MMILMNTLDYGRNPAHLQFWFIMGMATVVGGFLAFPINNWLVKKKLKHGCMTLPEGEE
                     RSEGHMPHHHGGHEEHLQAQAERIDSKVIHGHTHEMGSLPLNTQLKYIGISFGILLII
                     VLIMAFFVPLKI"
     gene            complement(1176..2471)
                     /locus_tag="U6309_002664"
     CDS             complement(1176..2471)
                     /locus_tag="U6309_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN5506080.1"
                     /translation="MDMDIKHKPLKRELSLFGATMMGLGSIVGTGVFVSIGIAAGVTG
                     PSVILAIALAALVATCNAFSSAQLAANHAVSGGTYEYGYRYLHPAFGFTAGWMFLCAK
                     SASAATAALGFAGYFIHLSGLETAPVIPIALAVSITLILLVLSGLRRTNRVNIIIVMI
                     TLITLTVFIISGLPDVMQRGTANLRPFFPVSKQEGLSHFFYATALMFVAYTGYGRIAT
                     LAEEVKNPGKFIPAAIIITLMMSAVLYILVGLVAIGAVGVEKLAQVTETNATPLESAA
                     QAINIPGLSVLVSIGACTAMLGVLLNLILGLSRVALAMGRKGDLPKIFCYVSETRHVP
                     VSAVIGVGVVIIALILTGSVETTWTFSAFTVLIYYAITNLAALHLPKEEQLYPPLFAI
                     GGLLSCLFLAFWVPIGVWITGISLLVLGLLWKVVANRFL"
     gene            complement(2524..3402)
                     /locus_tag="U6309_002665"
     CDS             complement(2524..3402)
                     /locus_tag="U6309_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5506081.1"
                     /translation="MINIRKLRLGDESTLETFLKSHADESMFLRSNLYHSGLSYHDKP
                     FHGDYFASFDNTKKISGVLAHYWNGNVIVQCPDFNVLTALVGTFQKTATREIAGILGE
                     DQQADVVMKQLQLEHVDYAVNYPDGLYALNLQNLAMPDNQDRYQLKVAKDCDKCLLYE
                     WLRAYQIEALGAEEGSSILKEQVESEVKNTLEGNDRWVLEENGVPVSLCGFNARLPDI
                     VQIGPVWTPVTLRGRGYARAVVALSLSAAQKSGVSRAVLFTNNQAAVRAYQAIGFNKV
                     GNYRLAILKKPILLTR"
     gene            complement(3399..4061)
                     /locus_tag="U6309_002666"
     CDS             complement(3399..4061)
                     /locus_tag="U6309_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEN5506082.1"
                     /translation="MKPTDKKHWSKIEYLHQSVKNPNIHIKGKHSYYSDAWTGSFEQT
                     VVRYLYGDEYSLKTWQPQWPIDQLYIGDYVCIAAEVIILLGGNHNHRADWFCLYPFAD
                     KYVDAYQGKGDTIIKDGVWLGMRAVIMPGVTIGEGAIVAASSIVTKDVEPYSIVAGNP
                     AKPVKKRFAENVIERILALDIYSWSEAKFNLLKDYICSNNIDKLEQASRSYDQNHTIE
                     SK"
     gene            complement(4259..>4602)
                     /locus_tag="U6309_002667"
     CDS             complement(4259..>4602)
                     /locus_tag="U6309_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506083.1"
                     /translation="EKTLSVFPAGTSINKTTVKEICFTDIFCETHPFVWYYYPEHNLA
                     IWTSRLAGTTQEYPADYLLNQLAKIPGITIDQGDAAIKDVSTFLQDHQMIPIRNSESW
                     RYIKSSSFSMS"
BASE COUNT         1458 a         1011 c          787 g         1346 t
ORIGIN      
        1 ataaaggagg agttatgttt aacttaaaaa ggaatttaat caagttaggg actctaagta
       61 tcagcattgc tctattagca gcatgccaca ctgtcaaggg gactgtaaca ggagctaaac
      121 aagatattaa agcaactgga aaagcaatat caagaaccat gcaataagag caaaacaatg
      181 ttggattatt ttaatccaac attgttcaga tttaataaaa atctctatta tattttcaat
      241 ggaacaaaaa aggccattat caaaacaata atcaataaaa ttccaaaact tataccgata
      301 tatttcaatt gagtgtttag tggtaagctt cccatttcat gggtgtgtcc atgaatcact
      361 ttcgaatcaa tcctttcagc ttgtgcctgt agatgctctt cgtgacctcc atgatgatga
      421 ggcatatgcc cttcgctgcg ttcttccccc tctggcaaag tcatacatcc atgctttaat
      481 tttttcttaa ccaaccagtt attaattgga aaagccagaa aaccgccgac tacagtagcc
      541 attcccataa taaaccaaaa ttgcaaatgt gctgggttgc gtccatagtc taatgtgttc
      601 attaaaatca tcatcacagg aatcataccc atcatcacaa aattcataga tatggtttct
      661 gcaaaaaatg ttttccttaa agcttctcca tagcttgaat acatacttct catcattcca
      721 gcctgaaaaa taacccaacc aaagaaaaaa gctgaaatat actcgagaat tagctctata
      781 ccattactga aatcaataaa agataaaaaa attgctgcga agatgatccc tgtcgcatca
      841 ccagccagac aatgcacttc ggagttaacc gcctgtttcc attgcgcttt ggtatataaa
      901 gcatgcaatc ctttacccgg gcttctgcaa gtcaaaaaat aaaaaaacaa gcctaaaggg
      961 ccggtatagg ccacgaccaa tacccatccg agtttttgca cccaatcaat tggcatctcc
     1021 ctcacatcat aagcaataaa agccaaagag agcatcagta aaataaacca taaaaccata
     1081 attccatcga gcattgcggt tccttttctc tgatcttctt gtttattcta gaatacattt
     1141 ttattctaga atacattcgt ctttttttct tgcctttata aaaaacgatt ggcaacaact
     1201 ttccaaagaa gtcccaggac aagtaaactg attcccgtta tccatacccc aattggaacc
     1261 caaaacgcta ggaaaaggca ggacagtaag cctccgatag caaacagagg tggatacaat
     1321 tgctcctctt taggcaaatg caaggcagcg aggttagtaa ttgcgtaata gattaaaact
     1381 gtaaacgcac tgaatgtcca tgttgtttca acgcttccag tcaatatcag agcaataatt
     1441 acaactccaa ctccaatgac agcggaaaca ggaacatgtc ttgtttctga tacataacaa
     1501 aaaatcttcg gtaaatctcc ttttctaccc atagctaaag ccacacgaga taacccaagt
     1561 attaaattga gtaatacccc taacatggcc gtgcatgccc caatggagac cagaacactc
     1621 aatccgggga tattaatggc ttgtgccgcg ctctctaaag gagttgcgtt tgtttcagtc
     1681 acttgggcca gtttttcaac ccctacagct ccaatagcaa caagacccac caaaatataa
     1741 agtacggcgc tcatcatcag agtgataata atcgctgcag gaataaattt tcctggattt
     1801 ttaacctctt ccgctaatgt tgcaattctc ccataaccgg tataagccac aaacattaaa
     1861 gcagtggcat aaaaaaaatg acttaatcct tcttgttttg aaacaggaaa gaagggcctc
     1921 agattagcag ttcccctttg catgacatca ggtaatccgc ttattataaa aacagttaag
     1981 gttatcaatg taatcattac aataataata ttcactctat ttgttcttct gagtccactt
     2041 aagactaaaa gtattagcgt gatagacact gctaaagcga taggtatgac aggcgccgtc
     2101 tctaaacctg acaggtgaat aaaataaccg gcaaagccaa gcgctgccgt tgcagcggaa
     2161 gcactcttcg cacataaaaa catccaaccg gcagtaaaac caaaagcagg atgtaaataa
     2221 cgatatccat attcataagt acctccacta acagcatgat ttgcagcaag ttgagcgctg
     2281 gagaacgcat tacaggtagc aacaagtgct gccaaagcaa tagcaagaat gacggatggt
     2341 cctgtgacac ccgccgcaat tccgatgctg acaaaaacac ccgtcccgac aatagagcca
     2401 agccccatca tcgtagcgcc aaatagactg agttcacgct tcaaaggctt atgtttaata
     2461 tccatatcca tcgttttatt tcctatcaat aaatactttt actgttttaa caggtattca
     2521 caattaacgt gttaagagta ttggtttttt taatatggcc aatcgataat tgcctacttt
     2581 attaaaacca attgcctggt aagctcgtac ggcagcttga ttattagtaa ataataccgc
     2641 tcgactcact ccactttttt gagctgcact aagactaagc gcaacaactg ctcgagcata
     2701 tcctcgcccc cgcaacgtaa ctggtgtcca taccggtcca atttggacaa tatccggtag
     2761 tcttgcgtta aaaccgcata gcgagacagg gacgccgttc tcctctaaaa cccaacgatc
     2821 attgccttcg agagtatttt tcacttcact ctctacttgc tcctttaaaa ttgaactgcc
     2881 ctcttcagca cctaaggctt cgatttgata agctctaagc cattcatata ataaacattt
     2941 atcacaatct ttggcgactt tcagttgata tctgtcttga ttatcaggca ttgccagatt
     3001 ctgtaaatta agtgcatata acccatccgg gtaattcaca gcataatcta catgctccag
     3061 ctggagttgt ttcatgacaa catcggcttg ctgatcttcg ccgagaatac ctgcaatttc
     3121 tcgggtagcc gttttttgaa aagtgcccac tagtgctgtt aaaacattga aatcagggca
     3181 ctgcacaatg acattgccgt tccaataatg ggctaataca ccgctaatct ttttggtgtt
     3241 atcaaatgaa gcaaaataat ccccatgaaa aggcttatca tgataagaca acccggagtg
     3301 atagagattg ctgcgtaaaa acatactctc atcagcatga gattttaaaa aagtttccaa
     3361 ggtggattca tcacctaatc ttaatttcct gatatttatc atttgctttc tatggtatgg
     3421 ttttgatcat agctcctgct ggcctgctct aatttatcta tgttattgct gcaaatatag
     3481 tcttttaata agttaaattt ggcttcgctc caggaataaa tatctaaggc cagaattcgt
     3541 tcaatcacgt tttctgcaaa tctttttttg actggtttcg caggatttcc agcaacaatg
     3601 ctgtagggct ccacatcttt ggttacaata ctgcttgcag ccactattgc tccttcgcca
     3661 atagtaacgc ctggcataat aacagcacgc atgccaagcc agacaccatc cttaataata
     3721 gtatcgcctt taccttgata agcatctaca tatttatctg caaatggata caaacaaaac
     3781 caatctgctc ggtgattatg gtttccacct aacaaaatta tcacttcagc agcaatacag
     3841 acataatcac caatatataa ttgatcaatc ggccattgtg gttgccaggt ttttaaactg
     3901 tattcatctc cataaagata ccggacaacg gtttgctcaa aactaccagt ccaggcatcg
     3961 ctataataac tgtgtttacc cttgatatga atgtttggat tttttacgct ttggtgtaga
     4021 tattcgattt tagaccagtg ttttttatca gtaggtttca tgccttgctc atttaggaat
     4081 ttaaatgagc tcattgtata ttattatgat cataactttc taatgtttca ttgcgtgtgg
     4141 ctctatgcaa tcaataagat gagagaaatc atttgaatat cccttgcaca tcatttgcga
     4201 aactgagcca gcaaaatctt ccattattaa gaacaagggc ctgctttcaa tctcttcatt
     4261 aactcattga gaaagaagaa gacttgatat atcgccagct ttcgctgttt ctaataggaa
     4321 tcatttgatg gtcctggagg aaagtactaa catccttgat ggctgcatca ccttggtcga
     4381 tggtaattcc aggaattttt gcaagttgat taagcaaata atctgcggga tattcttggg
     4441 ttgtccctgc cagtcttgaa gtccaaattg ccagattatg ttctggatag taataccaaa
     4501 caaaaggatg ggtttcacaa aagatatctg taaaacaaat ttcctttacc gttgttttat
     4561 ttatagaagt acctgcagga aatacgctta atgttttttc cg
//
LOCUS       DASLOE010000200         4593 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.201, whole genome shotgun sequence.
ACCESSION   DASLOE010000200 DASLOE010000000
VERSION     DASLOE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.201"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            86..310
                     /locus_tag="U6309_002668"
     CDS             86..310
                     /locus_tag="U6309_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506084.1"
                     /translation="MSKFLVFIGSILFFSSVLAGDIPEVDIQDQQSDQELCTQKFYNQ
                     CINKCEKTNYGDCTQACEENAKNQCLYAGE"
     gene            440..1012
                     /locus_tag="U6309_002669"
     CDS             440..1012
                     /locus_tag="U6309_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEN5506085.1"
                     /translation="MLHNTKINKLAVFCGSNTGYSEVYKQSAENMADVLSSNGITLVY
                     GGSKSGLMGIIANRMLENGSNVIGVIPKSLVDTEKAHNGLTELHVVNSMYERKILMCK
                     LSDGFILLPGGSGSLDEFFEMFTLVQLGYHKKPCCVLNASGYYDYLLQFLDYAVNQGF
                     LRSKDRNTLIVNQYPDTLIENLFEVINNRK"
     gene            1052..1792
                     /locus_tag="U6309_002670"
     CDS             1052..1792
                     /locus_tag="U6309_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEN5506086.1"
                     /translation="MPKKLLIAAFLCNIFCNPSHLAYASSTEIPILGKTMTQAKQQQM
                     TPRQALQRLKDGNQRFLSNKPLARDYLKQAKQSAYGQYPFAVILNCMDSRSVPEFFFD
                     QGLADLFTLRVAGNVLNDDILGSMEFATKVVGARLVVVLAHTSCGAVAGACKDVKLGH
                     LTDVINKIHPVVKPSMESTGIDNCSDPKLIDDMAKANALHVVKNILEQSPILNELVKN
                     KQIGIVAGIHDIKTGKVTFFEEKRSVPE"
     gene            1889..3109
                     /locus_tag="U6309_002671"
     CDS             1889..3109
                     /locus_tag="U6309_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEN5506087.1"
                     /translation="MPKSHNSIIFLGLLSAFSLLTFDLYQPSLPFITNYFNITPSMGQ
                     LTLSIYLLVYGITHLLWGPLIDHFGRRRLLPGNLLLALIGSLMCAFAPNITMLICGRA
                     LQGFALCCANLIAYSAARDFEDTIVRAKVLSYISMTVSVSPIIAPVIGALIFHHFGWQ
                     ANFIVMAIVACTLLIQTKLSLLESPFWTPPQERFSVKKILQEYKSILNIPSLWCASFI
                     LMFGISAVMLTVINSSYLIIEVLNYSPLAYGLIFILNGLNIIFGNYLGIWLRDRLPII
                     TTIYLGNWFIVLGGTAMLVTSKLFGFSLLALSFSLIANLGISVSAAPTISIALTDFKQ
                     NAGIVMAFMNTIRLIGPSLLTILTGYLLTRNLDALPLGLIGSGIGALFFSWHFSRLTT
                     ESRNSDMDSEEALT"
     gene            complement(3106..4371)
                     /locus_tag="U6309_002672"
     CDS             complement(3106..4371)
                     /locus_tag="U6309_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506088.1"
                     /translation="MKPPSRKLGYWEELCQWGNDKDHGNGLIAAVAKITVSDLDLERL
                     TNALRLLQIQQPLLRAKVIKSNQQCQFNIEEGDAVYLPFQIIKRTSNSVWKEVIELEL
                     NRPPRNQDYQWRTVLIYDQHGISHEILLIAGHNIGDGVSVAYFFDTLFRIYQKSDSNP
                     SFPLYPPVEELVQHKKGQKSSLPPAFLPTPIYYQNYVPPGERITKNHYYPVTVKQTGS
                     LLAFSKNHNFSLNALINALSLMTLTKVLDQPLDTTLHTPVNLRKWCMPVIPEDYFGCY
                     VSVVTTYHKQVSIESDIIELARDYDHQLKQERLPGDAIFPVHHSKQDIIDALSSWEGH
                     RDRFSMGVTVTNIGLLPFNTQYGHLKWGKSYFCTSRQNADIPVLLNVLTLNDQLMFCF
                     TFTEPMCGSVFQENYVKNFISNMGQLTDC"
BASE COUNT         1370 a          895 c          841 g         1487 t
ORIGIN      
        1 cttggagttt ttcctaagtc ctattatatt taatagtata atatttaatc tattttggat
       61 taaataaacc agggaaaacg cgatcatgag caaattcctc gtatttattg gatcaatatt
      121 atttttttct tccgttttag ctggagatat cccagaagta gacatacaag accaacagtc
      181 agatcaggag ttatgcaccc aaaaatttta taatcaatgc atcaacaaat gcgaaaaaac
      241 aaattatggt gactgtactc aagcctgtga ggaaaatgct aaaaatcaat gtctttacgc
      301 aggtgaataa gaatggtttg cgttgaggca attaaattaa atcctataaa ttttaatagt
      361 gaaaacatta actcattaaa aataaactgt atttcgtcat ttcatgttat aaataaaaca
      421 ttgcatctgg aattttaaaa tgctccataa taccaaaata aacaaactcg ctgtcttttg
      481 tggttctaac actggttatt ctgaagtcta taaacaatca gctgaaaaca tggccgatgt
      541 cctaagcagt aatggtatta ccctggttta tggtggctca aaaagtggtt tgatgggaat
      601 aatagccaat cgcatgttag aaaatggctc taatgtgatt ggtgttatac caaaatctct
      661 ggttgacaca gaaaaagcac ataatgggtt gacggaatta catgttgtta attctatgta
      721 tgaaagaaaa atattgatgt gtaaattgtc ggacgggttc attctgctgc caggtgggtc
      781 aggttctctg gatgaatttt ttgaaatgtt taccctggta caattagggt accataaaaa
      841 accttgctgt gtactcaatg cctctggtta ctatgattac cttttgcaat ttttagacta
      901 tgctgttaat caaggttttt tacgctcaaa ggatcgaaat acgctcattg tcaatcaata
      961 tccagatacg ctcattgaga atttatttga agtaatcaat aatcgcaaat aacagtttag
     1021 gaaaattaat aacataacaa ggagttaacc aatgccaaaa aaactgctca tcgctgcttt
     1081 cttatgcaat attttttgta accccagcca cctagcctat gcctcatcaa cagaaatacc
     1141 catacttggg aaaaccatga cccaggctaa acaacaacaa atgaccccca ggcaggcatt
     1201 gcaacgttta aaagatggta atcaacgctt cctaagtaat aaacctttag cacgagatta
     1261 tctaaaacaa gccaagcaat cagcttatgg ccaatatcct tttgcggtca tcttgaattg
     1321 tatggactcg cgcagtgttc ctgaattctt ttttgatcaa ggattggcag acctgtttac
     1381 cttacgtgtt gccggcaatg ttctcaacga tgacatttta ggaagtatgg aatttgcaac
     1441 aaaagtagtg ggggcacgtt tggtagttgt cctggctcac acctcctgtg gtgctgttgc
     1501 aggggcatgc aaagatgtta aattagggca tttgactgat gttataaata aaatccatcc
     1561 tgttgtaaaa cccagcatgg aaagtacggg cattgataat tgctctgacc ccaaactgat
     1621 tgatgacatg gctaaagcca atgccctgca tgttgtaaaa aatatactag agcaaagccc
     1681 tattttaaat gaattagtta agaataaaca aattgggatt gtagcgggta ttcatgatat
     1741 taaaacaggt aaggtgactt tttttgaaga aaagcgatct gtcccagagt aaagcaagtt
     1801 ggcaattgcc aacttgcttt aatatttcct ttcaaatgca agctgaattt ggttacaatc
     1861 gcataatttt tggtgaatga ttttttatat gcctaaatca cataattcca ttatattcct
     1921 tggtttgctt tctgcgtttt ctttgcttac atttgatctt tatcaaccat cactgccttt
     1981 cataacgaat tattttaata ttaccccaag tatggggcaa ttaaccctaa gtatttattt
     2041 gctagtttat ggcatcactc atcttctatg gggaccactt atagaccatt ttgggcgccg
     2101 acgcctactt cctggaaacc ttttattagc cttaattggc agtttaatgt gtgcttttgc
     2161 gccgaatatc accatgctta tttgcggtcg tgccctacaa ggattcgcct tatgctgcgc
     2221 aaatctcatt gcctattccg cagcacgtga ttttgaagac acgattgttc gcgccaaagt
     2281 gctgtcctat atttccatga ccgtttcagt ttcaccgatt atagcccctg taattggtgc
     2341 tcttatcttt catcattttg gctggcaagc caattttata gtcatggcaa tcgttgcctg
     2401 tactctattg atacaaacca aactctcctt gttagagtcc cctttctgga cgcctcccca
     2461 ggagaggttc tcggtaaaaa aaatactcca ggaatataag tccatactca atatcccctc
     2521 tctatggtgc gcatcattta ttttaatgtt tggcatttct gctgtcatgc tcactgtaat
     2581 taattcctct taccttatta tcgaggtact gaattactct ccacttgcct atggacttat
     2641 ttttattctg aatggtttaa atattatttt tggaaactat ctgggcattt ggcttcgtga
     2701 ccgattaccc attataacca ccatctattt ggggaactgg ttcattgtcc ttggcggtac
     2761 agcgatgttg gttacttcca aattgtttgg gtttagtctg ttagcattat cattttcatt
     2821 aattgccaac ttgggaatta gtgtcagtgc tgctccaact atctccatcg ccctaacgga
     2881 ttttaagcaa aatgcaggaa tagttatggc ttttatgaat accatacgtt taatcggccc
     2941 ctctctttta actattctaa ccggctattt gttgacaagg aatcttgacg ctcttccact
     3001 gggattaata ggctcaggaa ttggagccct ctttttctca tggcatttta gccgattgac
     3061 cactgaatcg agaaactcag acatggatag cgaagaagcg ttgacctaac agtctgttaa
     3121 ctgccccata ttggatataa aattttttac ataattctcc tgaaacacag aaccacacat
     3181 tggttctgtg aaggtaaaac aaaacatgag ttgatcatta agagtaagaa cattgagcaa
     3241 aacaggtatg tctgcatttt gccgggatgt acaaaaatag ctttttcccc atttcaaatg
     3301 accatattga gtattaaaag gtaacaaacc aatattggtt acggtcactc ccattgaaaa
     3361 gcgatcccta tgaccttccc atgatgacag agcatcaata atatcctgct tactgtgatg
     3421 aaccgggaat atagcgtcac caggcaatcg ctcttgtttt aactgatgat cataatccct
     3481 ggcaagctct ataatatctg attctatact gacttgttta tgatacgtcg tcaccacaga
     3541 cacatagcag ccaaaatagt cctccggaat cacaggcata caccacttcc ttaaattaac
     3601 cggggtgtgt agtgttgtat ctaaaggctg atcgagaact tttgttaatg tcattaggga
     3661 cagcgcatta attaatgcat tcagagaaaa gttgtgattt ttactaaaag ccagaagcga
     3721 tccggtttgt ttcactgtaa caggataata atgatttttt gttattcgct cgccaggagg
     3781 gacatagttt tggtaataaa tcggagtagg caaaaatgca ggaggcagag aagatttttg
     3841 cccttttttg tgttgcacca gttcctcaac agggggatag agagggaagg atggatttga
     3901 atcacttttc tggtaaattc taaagagagt atcgaaaaaa taggctaccg agacgccatc
     3961 tccaatatta tgtcctgcga ttaacaggat ttcatgagaa ataccgtgtt ggtcataaat
     4021 aagaacggtt cgccattgat aatcttgatt acggggaggt cgatttaatt ccaattcaat
     4081 cacctctttc cagacagagt ttgaagtcct ttttatgatt tgaaatggta aataaacagc
     4141 atcaccttcc tctatattaa attgacactg ttgattgctt ttgatcactt ttgctctaag
     4201 caatggctgt tggatttgta ataacctgag cgcgttggtt aaacgctcca agtccaaatc
     4261 agaaacagtg attttcgcaa cagcagcaat taaaccattt ccatgatctt tatcgtttcc
     4321 ccattgacat aactcttccc aatagcctag ttttctggat ggcggtttca tagattttct
     4381 cccgaagccc tcgtcatgat tctggtaagt tccttttaca gagagcaatt tccatactac
     4441 aggacatgca cctgcttaat tacttttgat ggctctaacc catattaatg ctctaaatta
     4501 taaataattg cttttttcaa gttctattta tgcaattaag tcaaaaaaac tcataatttt
     4561 atcaacatat tatcatcttg ctattcctga tta
//
LOCUS       DASLOE010000201         4568 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.202, whole genome shotgun sequence.
ACCESSION   DASLOE010000201 DASLOE010000000
VERSION     DASLOE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.202"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..838
                     /locus_tag="U6309_002673"
     CDS             <1..838
                     /locus_tag="U6309_002673"
                     /inference="COORDINATES: protein motif:HMM:NF013752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEN5506089.1"
                     /translation="RASFAATGRGITLYEEVHPRAKLGNEQVQSNFLMNLATILPFGC
                     KPSIITDAGFKIPWFKMVLKLGWNYIGRVRGEVQFNEDGEFKPISHLFPRASTKALYV
                     GNLVLAKYNPFKTNFYLYTHKLKGRKKTNRNGTFAKDKESIVHSKGYREPWVIVSSLE
                     LHTHPEEIIKKYKSRMSIEEALRDTKSILYGFSFNINQTMKQERYIVWLLLAALAAVV
                     AWIVGFVAEKNKLHHQFQSNTYRHKRVLSFVFLGCRIINKNIQILFEYDSLSIIFQGE
                     QK"
     gene            complement(872..2248)
                     /locus_tag="U6309_002674"
     CDS             complement(872..2248)
                     /locus_tag="U6309_002674"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEN5506090.1"
                     /translation="MNISVFDLFSIGIGPSSSHTVGPMLAANAFLQLLEQKNLFDKTQ
                     RVKVELYGSLALTGKGHGTDKAILNGLENKAPETVDPASMIPRMHEILDSNLLNLAGK
                     KEIPFHEATDFLFLQKELLPKHSNGMRFSAFDGNTNLLIEQVYYSIGGGFITTEEDFD
                     KSTTDTNPPPYPFATATELLKLCKKHHLTIAELMLVNEKTWRSTAEIHKGILDIAKVM
                     DDCINNGCKHDGVLPGGLNLKRRAPDLYRKLIEQKGVKSIFEQSDIMNHLNLYAMAVN
                     EENAAGGRIVTAPTNGAAGIIPAVLKYCQQAHDRMSNEDIYTYFLTAAAIGILYKKGA
                     SISGAEVGCQGEVGVASSMAAAGLTAVLGGTIEQVENAAEIAMEHHLGMTCDPVLGLV
                     QIPCIERNAMGAVKAVNATRMALIGDGQHQISLDKVIKTMKQTGMDMQSIYKETSMGG
                     LAVNLPEC"
     gene            2453..3154
                     /gene="mip"
                     /locus_tag="U6309_002675"
     CDS             2453..3154
                     /gene="mip"
                     /locus_tag="U6309_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrophage infectivity potentiator Mip"
                     /protein_id="HEN5506091.1"
                     /translation="MKMKLVTAAVMGLAMSTAMAATDATSLATDKDKLSYSIGADLGK
                     NFKNQGIDVNPEAMAKGMQDAMSGAQLALTEQQMKDVLNKFQKDLMAKRTAEFNKKAD
                     ENKVKGEAFLTENKNKPGVVVLPSGLQYKVINSGNGVKPGKSDTVTVEYTGRLIDGTV
                     FDSTEKTGKPATFQVSQVIPGWTEALQLMPAGSTWEIYVPSGLAYGPRSVGGPIGPNE
                     TLIFKIHLISVKKSS"
     gene            3320..4567
                     /locus_tag="U6309_002676"
     CDS             3320..4567
                     /locus_tag="U6309_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5506092.1"
                     /translation="MNKRLFIVFILGFSSGLPMALISSTLQAWYANNGMSVLATGALS
                     LVSLPYAYRIFWGPILDRYSLFHLGKRRSWILTMQILLLLGFNLMAWFIPAQYPKLMA
                     FLALVLACFSATQDVAIDAHRAEYLPPQEHALGASLAVFGYRLALLLSGGLALVMAEK
                     LGWAFTYRFMGFAMAIGMLAIFVSKEPSTEIKEKSNFLVSFIAPVKELLSRPGIIYLL
                     IFIFCYKLGEAFTTTTSGIVMPFLIQGLGFSLDTIGYINKMLGIVSILLGGLTAGFLL
                     MRYSLYRALFFFGLLQALTNVFFVILAMSGKNIVLLAIAVFSDNFVAGMGSTALVALF
                     MRLVNKQFTGTQFSMLVALSTLPRIISGPVAATIQMHIGWVGLYQLSIVFALFFIPFL
                     VLIKEQTKERLQSDTAATPIGAA"
BASE COUNT         1317 a          859 c          943 g         1449 t
ORIGIN      
        1 cagagcttct tttgccgcaa caggtcgagg tattaccctt tatgaagaag ttcatccgcg
       61 agcaaaactt ggtaatgagc aagttcaaag taactttttg atgaatttag caactatcct
      121 cccttttggg tgcaaaccaa gcattattac cgatgcggga tttaaaattc cctggtttaa
      181 aatggttctt aagttgggct ggaattacat tggacgtgtg cgaggagagg tacagtttaa
      241 tgaggatggt gaattcaaac caataagcca tttgttccct agagcctcta ctaaagcact
      301 ctacgtgggg aatttggtct tggcaaaata taatccgttc aaaacaaatt tttatcttta
      361 tacgcacaaa ctcaaaggta gaaaaaaaac gaatcgaaac gggacatttg ccaaagacaa
      421 agagtcaata gttcactcta aagggtatag agagccatgg gtgattgtat cctcactgga
      481 actccatact catcctgaag aaattataaa aaaatacaaa tcaagaatga gtatcgaaga
      541 agccttgaga gatactaaaa gtattttata tggatttagc ttcaacataa atcagacaat
      601 gaaacaagag cgttatatcg tctggctatt attagcagcg ttagctgctg tagttgcttg
      661 gattgtcgga tttgttgcag aaaaaaataa gctgcatcat caatttcaat ccaataccta
      721 tcggcataaa agagtcttgt cctttgtatt ccttggctgt cgcatcatca ataaaaatat
      781 tcaaatccta tttgaatatg actccctgag tattattttt cagggagagc aaaaatgagt
      841 cgatccctca gccgtgaggg agcggtgata actaacactc aggcaagtta acagccaatc
      901 cacccatgga cgtttcctta tatatacttt gcatatccat gccggtttgc ttcattgttt
      961 tgatgacttt atccaaagag atttgatgct gaccatcacc tatgagtgcc attcgggttg
     1021 cattaactgc tttcacagcc cccatggcat ttctctcgat gcatgggatt tgcacaaggc
     1081 ccagaacggg atcacaggtc attcccagat gatgttccat tgcaatttct gcagcatttt
     1141 caacctgctc gatggttccg ccaagaaccg ctgtcaaacc agcagctgcc atggaagagg
     1201 caacaccaac ctcaccttgg catcctactt cagcgccaga aattgaagcg ccttttttat
     1261 aaaggattcc aatcgctgcg gcagtcagaa aataagtgta gatgtcttca ttactcattc
     1321 tgtcatgagc ttgctgacaa tatttaagaa cagctggaat aattccggca gctccatttg
     1381 ttggggctgt gacaattcgt cctccagccg cgttttcttc attgacagcc atagcataaa
     1441 ggtttagatg attcataata tctgactgct caaaaatgct tttaactcct ttttgttcta
     1501 ttagttttct atacaagtca ggtgcgcgtc gctttaaatt aaggccaccg ggaagcactc
     1561 catcatgctt gcagccgttg ttgatgcagt catccattac tttggctata tcaagaatac
     1621 ccttatgaat ttctgctgta cttctccatg ttttttcatt gactaacatc aattcagcga
     1681 tggtcaagtg atgttttttg cataatttca ataactcagt tgctgttgca aaggggtaag
     1741 gggggggatt ggtatcggtt gttgatttat caaagtcttc ttcagttgta ataaaaccac
     1801 cgccaataga gtaatagact tgctcaatca gtagattagt gtttccatca aaagcagaaa
     1861 agcgcattcc attactgtgt ttgggtaata attctttttg taaaaataaa aaatcggtcg
     1921 cttcatgaaa aggaatttct ttcttgccgg ccaaattgag tagattagaa tcaaggattt
     1981 catgcattct tgggatcatg gaggcaggat caactgtctc gggagctttg ttttcaagac
     2041 catttaaaat ggctttatca gtaccgtgcc ccttgccagt aagcgccagc gaaccataaa
     2101 gctctacttt gactctctga gttttatcaa acagattttt ttgttccagt aattgcaaaa
     2161 aagcattggc agctaacata gggcctactg tgtgtgagct tgatggccca attcctatag
     2221 aaaataaatc aaaaacacta atgttcatcg ccgttaaaat ctcttgttca ttattagggg
     2281 caagtgtaga aggatattac ctttttgtcc attatataat taaatgatag cttatgactg
     2341 gtaattttac gcaaatttag gcaaaattag tgggcgattt gtttttgctt tattttgctc
     2401 aatttattgt gcagtatgag aacttaagtg taagactaaa aggggattgt ttatgaagat
     2461 gaaattggtg actgcagctg ttatggggct tgcaatgtca acagcaatgg ctgcaaccga
     2521 tgccacatca ttagctacag acaaggataa gttgtcttat agcattggtg ccgatttggg
     2581 gaagaatttt aaaaatcaag gcatagatgt taatccggaa gcaatggcta aaggcatgca
     2641 agacgctatg agtggcgctc aattggcttt aaccgaacaa caaatgaaag acgttcttaa
     2701 caagtttcag aaagatttga tggcaaagcg tactgctgaa ttcaataaga aagcggatga
     2761 aaataaagta aaaggggaag cctttttaac tgaaaacaaa aacaagccag gcgttgttgt
     2821 attgccaagt ggtttgcaat acaaagtaat caattctgga aatggtgtta aacccggaaa
     2881 atcggataca gtcactgtcg aatatactgg tcgtctgatt gatggtaccg tttttgacag
     2941 taccgaaaaa actggtaagc cagcaacgtt ccaggtttca caagttatcc ctggatggac
     3001 agaagctttg caattgatgc ccgctggatc aacttgggaa atttatgttc cctcaggtct
     3061 tgcatatggc ccacgtagcg ttggcggacc tattggccca aatgaaactt taatatttaa
     3121 aattcactta atttcagtga aaaaatcatc ttaagttttt ttgaattaaa gtcatacaaa
     3181 acgcatcctt ctcatttaga gagggatgct ctctttgtaa aggctaatga tcttcataaa
     3241 aggtgtcagc ctaacaccac tgaggaaatt aaaatatgtc tgttttaaag gctatgactc
     3301 atactgagtg aacaaaggaa tgaacaagcg cctgtttatc gttttcattt taggtttttc
     3361 ttccggttta cctatggcat taatcagcag taccttgcaa gcatggtacg ctaataatgg
     3421 catgtcagtg cttgcgacgg gagctttaag tttagtcagt ctaccttatg cgtatcgtat
     3481 tttctggggt ccaattcttg atcgctactc tttatttcat ttagggaaaa ggcgaagttg
     3541 gattctcacc atgcagatac tactgcttct gggttttaat ctcatggcct ggtttattcc
     3601 agctcaatat cctaaattga tggcattttt agctttagta ttagcctgtt tttcagccac
     3661 acaagatgtg gcaattgatg ctcatcgagc agaatatctt cccccacagg aacatgcatt
     3721 gggcgcgtct ctggctgtgt ttgggtatcg attagctctt ttgttatctg gcggtttggc
     3781 tttggtaatg gcagaaaaat taggctgggc ttttacttat cgctttatgg gatttgcaat
     3841 ggcgataggt atgttggcaa tatttgtcag caaggaaccc tctacagaaa taaaagaaaa
     3901 aagtaatttt cttgtttcgt tcattgcacc agtgaaagaa cttttaagcc gcccagggat
     3961 tatttatctt ttaatattta tattttgtta taaattaggt gaagcattca ctacgacaac
     4021 tagcggtata gtgatgcctt ttttgatcca aggcttgggt ttctctctgg atactatcgg
     4081 ctatatcaat aaaatgcttg gaatagtctc tatcctgtta ggcggtttaa cggcaggttt
     4141 tttattaatg cgatattcct tgtatcgcgc attatttttc tttggcttac tacaagcact
     4201 aaccaatgtc ttttttgtca tattagcgat gtcaggaaaa aatattgtgc tgttagcaat
     4261 cgcagtattt tcggataatt tcgtcgcagg gatgggttct actgcattgg ttgctttatt
     4321 tatgcgtttg gtgaataaac agtttactgg aacacaattt tctatgttag tcgcattatc
     4381 gactttaccc aggattattt caggccctgt cgccgcaacc attcaaatgc atatcggctg
     4441 ggttggttta tatcagttat ctatcgtttt cgctttgttt tttatacctt ttctagtgtt
     4501 gatcaaggag caaacaaaag agaggttaca aagtgataca gctgctactc caattggtgc
     4561 agcttaat
//
LOCUS       DASLOE010000202         4424 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.203, whole genome shotgun sequence.
ACCESSION   DASLOE010000202 DASLOE010000000
VERSION     DASLOE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.203"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            87..1055
                     /locus_tag="U6309_002677"
     CDS             87..1055
                     /locus_tag="U6309_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1137 family Dot/Icm T4SS effector"
                     /protein_id="HEN5506093.1"
                     /translation="MIQRGFTMQERREKQGNNSPYLLTPYEMANLVFKTGAISFTVSA
                     GTQPFQYLLNKLQFSQSGTPSGLSGGLFRGMYRGFLPYAIAGQKRGAVAVTHKQTNKA
                     TEEEEFEAPFRQRWLGTIFFSQADLLVSNGLSGKARLQNVGVINAENFKWSLSNFWKL
                     TSVNWGSRSFAGGVNFALIGFAGDYVSSFYKFDKDLYNKILGGATSGVIATLFTTVPN
                     AYADSKLLQTKVAENNRLITVSPYTMFGQMKSHVKAVGLKEAFMTFFKVGYLQQVAIR
                     APQAAITFALIFGMDEYMGPQPLKKVWPGRVEELESETPSPSPAKK"
     gene            1304..1501
                     /locus_tag="U6309_002678"
     CDS             1304..1501
                     /locus_tag="U6309_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506094.1"
                     /translation="MLKTLGGATRTAALDTARVTATTTVVLGTMYFWDRVAKNYGLHD
                     KENSKPISLDLGNDEYMRPKI"
     gene            complement(1587..2492)
                     /locus_tag="U6309_002679"
                     /pseudo
     CDS             complement(1587..2492)
                     /locus_tag="U6309_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841375.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
     gene            2662..3273
                     /locus_tag="U6309_002680"
     CDS             2662..3273
                     /locus_tag="U6309_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN5506095.1"
                     /translation="MKKIAPTLKNEIINTAIDIAQDTSWEKVRLVDIAKALNIDLNTI
                     RYFFAEKEQIIDGCFDRADEAMLNASSRPKFDLLTAKEKLHDLLMTWLSTFEGQQKVI
                     RQMIFHKLEPGHLHLQLPAIKRISQTVQWWREAAGLRETFIQRAIQETGLTIIYLTTF
                     SYWLLDNSTEHLATRLFLENKLNMAKNLSNFWGFTYYAPSKIS"
     gene            3251..4303
                     /locus_tag="U6309_002681"
     CDS             3251..4303
                     /locus_tag="U6309_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEN5506096.1"
                     /translation="MRHLKLAERLCIQFPIIQAPMAGGATTPELVAAVSNSGGLGSLG
                     AGYMRPDEIRQAIIKIRQLTSKPFAVNLFIPEAHHATPEQIQSACDDINLCCTELNIE
                     ISPVSKPYSLPFVDQMQILIEEKIPVFSYAFGTLEPMWIKQLKKNGTFLIGTATTIHE
                     ARILEASGIDAIVAQGSEAGGHRGTFIGNAEEALTQLSELIPQLVETIRVPVIAAGGI
                     MNGKGIISAIHSSASGVQMGTAFLSCFEAGISYKYKQTLLSQQQDNTVLTRAFSGKLA
                     RGIRNKFITCMDNRKINILDYPIQNALTQVMRQKARENDNIDFMSLWAGQSAHLCRSM
                     SASDLISALVIEAEMK"
BASE COUNT         1443 a          831 c          810 g         1340 t
ORIGIN      
        1 atattatttt catacttaat attttttcca caccagttaa tatttgctta ataataaatt
       61 gttatcatta cagcacattg atttaagtga ttcaaagagg atttacaatg caagaacgta
      121 gagagaaaca gggtaataat tcaccctatc tgttgacgcc ctatgaaatg gccaatttag
      181 tttttaaaac aggagctatt tctttcactg tttctgcagg aactcaacca tttcaatact
      241 tgctaaacaa attgcaattt tcacaatctg gaactccatc tggattgagc ggaggacttt
      301 ttcgaggcat gtaccgtgga ttccttcctt atgctattgc aggtcaaaaa cgcggtgctg
      361 tggctgtaac ccataaacaa actaacaaag caacagaaga ggaagaattc gaagccccat
      421 tccgtcaacg ttggttggga accatattct tttctcaggc tgatctattg gttagtaatg
      481 gtttaagtgg aaaggcaaga ttgcaaaatg tcggcgttat taatgctgaa aactttaaat
      541 ggtccctttc caacttttgg aaattaactt cagtcaactg gggttctcgt tcttttgccg
      601 gtggggtaaa tttcgcattg ataggatttg caggagacta tgtcagctca ttttataagt
      661 ttgataaaga cttgtataac aaaatattag gcggcgctac ttctggcgtc attgcgacat
      721 tatttacaac cgtccctaat gcttatgcgg atagtaaatt gttgcaaacc aaagtggctg
      781 aaaataaccg tttaattact gtgtcaccct ataccatgtt tggccaaatg aaatcacatg
      841 taaaagcggt tggtcttaaa gaagccttca tgaccttttt caaagttggc tatttgcaac
      901 aagtagcgat aagagctcct caagcagcca tcacttttgc cctcattttt ggtatggatg
      961 aatatatggg tcctcaacct ttgaaaaagg tttggcctgg acgagtcgaa gagcttgagt
     1021 cagagactcc atccccatcc cctgccaaga aataattaag agtaacaagg atgttatttt
     1081 tcatcattgt gccttaacaa acaacaatac gttatactgc ataaattgta cttatttaat
     1141 ctacatgatg ttaaaaaaaa catgattgat ttaattttaa tcatacaagt aggggaatgt
     1201 attgcattag gcagaaattc atttagaata aatgatgaat aattagcagg attattatta
     1261 actaatcagg acggtaaata tgttatcatt ctttgggaaa tgtatgttaa aaaccttagg
     1321 cggagcaacc aggacagcag cacttgatac tgcgagagta acagcaacaa caacggttgt
     1381 tctaggtact atgtacttct gggacagagt agctaaaaat tatgggctac atgacaagga
     1441 aaattccaaa ccaataagcc ttgatctggg taatgacgaa tatatgcgcc ctaaaattta
     1501 aactaaaaga ttattttcgc tcccaactat accgttggga gtatttagct ctctcttaaa
     1561 ccaaaatctg agatacactg taaatcctat tgtttaaaac gggaataaac ggccctacct
     1621 atccaaaata ctaataaagc tataatcccc cacaataaaa tcattgggac aaataagata
     1681 acgaattcaa tgaatccgta ggtaaactgt cttaagtgct ctattaatgc ctggtaagat
     1741 tttctaacaa cttctgatat ttgccatgtt gaattttgtg cattttttat tgccggattg
     1801 atctgcaagt aaattgtaat gagagaatag gcaacagatt ctttatagta ctttatctac
     1861 ccttctataa catcaatttt tctctgcgtt tcactcagtt gttgataaac agcaagcaca
     1921 tctgaagtgt ttttggcgcc ttgcataatc ttatttaatt gatctttagc aaccctgtaa
     1981 tttttcagct cactttcaag atcaacatat tgccgggtga tgtcttcact cgtaattgat
     2041 tcctcaatca ctttaacagc taaagatttt aaattttgca atacattgtt taaacgatta
     2101 cttggtattc tgatgctgat ttgagctata ttgccaccaa catagaaact atcctgtctt
     2161 aaattagagt taaccgcata tcctccctct cgctcggcca tctgcataat ttcatcaata
     2221 actttagccg gatcattgac ctccagagtg atattagcat tacgaattat cattcctttc
     2281 atgacttgat ctgaagcaga aggattattg ttgctatcac cagaaccacc tggtaccgca
     2341 ttgacttttt caacagtggg ataggcactc cccattgctg gagcgccccc atataatgtt
     2401 tgtccagctc gatttatcag catgttgtac gcaaatagaa ggacagcgta aactccaata
     2461 gcgacaaaca agtataaaat gattttcttc atttttaatt caatactccg atattataaa
     2521 aataagtgtg gcatagacta attgaaataa ccaatagtat tttttgattt aacttatagt
     2581 taatttaact accaaaacca tcaaaaatta atcctgatca taaaattagt aataaactaa
     2641 taagagacag ggaggtgttt catgaaaaaa atagcaccaa cactaaaaaa tgagatcatc
     2701 aatacagcaa tagacattgc ccaggatacc tcttgggaaa aagtgaggct ggttgatatc
     2761 gctaaagcat taaacatcga tttaaatacg atccgatatt tttttgcgga aaaagagcaa
     2821 atcattgatg gctgttttga tcgtgctgat gaagccatgc tcaatgcgtc ctccagacca
     2881 aaatttgatt tactcactgc caaagaaaaa ctgcatgatt tattaatgac ttggttaagt
     2941 actttcgaag gccagcaaaa agtaatccgg caaatgatat ttcataaatt agagcctggt
     3001 catcttcatc ttcaattgcc tgcaatcaaa cgcatcagcc aaactgtcca atggtggcgt
     3061 gaagcagctg gtcttcgaga aacctttatt caacgtgcga tccaggaaac cggattaacc
     3121 atcatttatc taaccacctt tagttattgg ttattagata attccacaga gcatcttgca
     3181 actcgtcttt ttttggaaaa caaattaaat atggcaaaaa atttatccaa tttttggggt
     3241 tttacttact atgcgccatc taaaattagc tgagaggctt tgtattcaat ttcctatcat
     3301 tcaagcacct atggccggag gagctactac tcctgagttg gttgcagcgg tttctaacag
     3361 tggtggtttg ggctctctgg gcgcagggta tatgagacct gatgagatca gacaagccat
     3421 tataaaaatt cgtcaattaa caagtaaacc ttttgctgtg aatcttttta tacccgaagc
     3481 acatcatgca acaccggaac aaattcaaag cgcttgtgat gatatcaatt tgtgctgcac
     3541 tgaactaaat attgaaatca gtcccgtgag taaaccctac tccctacctt ttgttgacca
     3601 aatgcaaatc ctgatagagg aaaaaatccc tgtctttagc tatgcattcg gtacattaga
     3661 accaatgtgg atcaaacaat tgaagaaaaa tggaaccttt ctgataggaa cggcaaccac
     3721 tatacacgaa gcaagaatac ttgaagcaag tgggattgat gccattgttg cacaaggaag
     3781 tgaagcagga gggcatcgag gtacttttat cggcaatgct gaggaagctt tgactcaact
     3841 atctgaactg atacctcaac tagttgagac aataagagtg ccagtgatag ccgctggggg
     3901 aatcatgaat ggtaaaggca ttatctctgc catccattca agtgcctctg gtgtacaaat
     3961 gggaaccgct tttttaagtt gctttgaagc tggtatctct tataaatata aacaaaccct
     4021 attatcacaa caacaagata atactgtgct aacacgtgct ttttctggaa agcttgctcg
     4081 tggaattcga aataaattca ttacttgcat ggataatcgg aaaataaata tccttgacta
     4141 ccccattcaa aatgcattaa cccaggtaat gagacaaaaa gccagagaaa acgataatat
     4201 tgattttatg tcgttatggg ctggacaatc ggctcattta tgccgatcta tgtcggcaag
     4261 tgacttaata agtgctttag ttattgaggc agaaatgaag taaaagagag gtgaatgatt
     4321 acaaaaacag ggcttacaaa aacctcaaag tcgaggcaaa aaatgttttt aatgagggag
     4381 tttagataaa ctaaatgacc gaattaaaaa ctttttttaa cata
//
LOCUS       DASLOE010000203         4406 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.204, whole genome shotgun sequence.
ACCESSION   DASLOE010000203 DASLOE010000000
VERSION     DASLOE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.204"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            165..1442
                     /gene="legC7"
                     /locus_tag="U6309_002682"
     CDS             165..1442
                     /gene="legC7"
                     /locus_tag="U6309_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC7/YlfA"
                     /protein_id="HEN5506097.1"
                     /translation="MATNETELQVLIQHDSKSTITTSSLDSTDKDPKASTGTPEIESG
                     SLAQIVDTQKQLSQVKESLESIVDSIAENPSLITRAASAWGELPMWQKVTGGIVLTAP
                     TLAVGLFAHIGVLLVIGGVTGLTYTAGAIVLDDHHTCNVNIAKRLKEGLFGLADLLQI
                     TIEALDAIRKKFAEEIEKFKNENLRLTDNIDRLGNEVESLSAQVELYMEIEKMLRKDT
                     NEMEQTVKKLQESTTKQTDLLEKNQKELSKIRKEYEKSQLQLAEKIAELHEVRLSLGL
                     EVQKAKTVAKTLEGTVQTLTGTVIADEEQRVSFQKKLDGFLNDKQLSFDQVAERICKA
                     EEELKKVKEELRQSNDRYSELLKRQEQQVERLEKLGLHKLERIVDKENVKPINDSVHS
                     GLLSHGIYSTPKGKVTQPKVEVVEDNKTIALVN"
     gene            complement(1621..3612)
                     /locus_tag="U6309_002683"
     CDS             complement(1621..3612)
                     /locus_tag="U6309_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEN5506098.1"
                     /translation="MEKMLINAMQTEEIRVALIKNNQLFDLDIECPGEIKKKGNIYKA
                     IVTRREPSLDAVFVEYGSKRQGFLPLKEIAPEYLSKNPEEFGDEKPPITSLIREGQEL
                     LIQVDKEERGNKGAALTTFITLAGCYLVLMPNNPHSGGISRRIEGDERDELRETLNAL
                     TLPEDMGLIIRTAGVGKSLEELQDDLNMLCNQWQSIKEAYNSELAPCLIHQEGDVIIR
                     SIRDNLRKSISEIIIDDQISYIKAKQYIERVKPEFLPNLKLYNSSIPLFNFYQIESQI
                     ETAYQRQVMLPSGGALVIDRTEALVSIDINSAKATGGADIEATALNTNLEAADEIARQ
                     LRLRDLGGLVVIDFIDMSSSKNQREVENRLKEALQADRARIQVGRISRFGLLEMSRQR
                     LRLSLGETAQEICPRCEGRGTVRSIQSHGLSITRLIEEEALKEKTAEIQVQLPPEMAT
                     FIMNEKRNFIRDIEKRHGVSVVVIANPYMHPPQYTITRLKEDNVGKSKKPSYSLIQQP
                     EVHVPSSDKEPVSHDEPAVKSFAEHRVIKHPQTSFIKRLWTSLFGGHSVAHTVTQEPG
                     KKHHRAQNQGKHASSKSSGGDKRQQGSNANRRRRSGGGQQRSSSGNTNAAASTHQQRK
                     KSNANQQSHGSQQSNRSIPAKNEGNKKREAILAKEQEEK"
BASE COUNT         1306 a          805 c          906 g         1389 t
ORIGIN      
        1 ataatgcctc attttgactt agtatttgaa ttttcagatc ttttttgtag taggtttatt
       61 aaaataaaat ttaattgcaa aaaatgcaag aattacttgt cttattgata atttatgatt
      121 aaaaatggac atctaaggaa gatttttaat cgtaaggagt aaatatggct actaatgaaa
      181 cagagcttca agtattgatc caacatgata gcaaatcaac aatcactact tctagcttgg
      241 acagtactga taaggatcct aaggcaagta ctggcactcc tgaaattgaa agcggtagtc
      301 ttgcccaaat cgtcgatacc caaaaacaac tgagccaagt taaagaaagc ctggaatcaa
      361 tagtagacag tatagcggaa aatcccagct tgattactcg cgccgcgtca gcctggggcg
      421 aactgcctat gtggcagaaa gtcacgggcg gcattgtcct gacagcgcca actttggcag
      481 taggattatt tgcgcacatc ggtgtcttat tagtcatagg gggagttaca ggactaacct
      541 ataccgcagg tgcaattgtt ttggacgacc accatacatg caatgtgaat attgccaaac
      601 gattaaaaga agggcttttt ggtttggcag atctgttaca aattacgatt gaggcattgg
      661 atgcaattcg caaaaaattt gctgaagaaa ttgagaaatt taagaatgaa aacctcagat
      721 taacggataa cattgatagg ctgggcaatg aagtggaatc actgagtgct caggttgagt
      781 tatatatgga aattgagaaa atgctccgta aagatacaaa tgaaatggag caaaccgtca
      841 aaaaacttca agaatctaca acaaaacaaa ctgatttatt ggaaaaaaat caaaaggaat
      901 tgtcaaaaat tagaaaggaa tacgaaaaaa gccaattgca gttagccgaa aaaatagctg
      961 aattacatga agtcagactg tcgttgggtc tcgaagttca aaaagctaaa acagttgcga
     1021 aaacgttgga aggtacagtc cagactttaa ctggaacggt tattgctgat gaagagcaac
     1081 gtgtttcttt tcaaaagaaa ctggatggat tcctgaatga caaacagctc agctttgatc
     1141 aagtagctga gcgaatttgt aaggcagaag aagaacttaa aaaagtcaaa gaagaattaa
     1201 gacaaagcaa tgatcgttat agtgaattat taaagcgtca ggaacaacag gttgaacgtt
     1261 tggaaaaatt gggcttgcat aaattggaaa gaatagtaga caaagagaat gtgaaaccca
     1321 ttaatgactc tgtccactca ggcttgcttt cccatggaat ctatagtacg cccaagggta
     1381 aagtcaccca gcctaaagta gaggtagtag aggataataa gactattgct ttagtcaatt
     1441 aagactcaaa taaacaggct tgtaaacaga aaaatttgca tatcccgtga atagttagaa
     1501 ttttaaaatc ggtatcctga caagaaagag tgctgtgttt tatccgcaca atccagaggt
     1561 aactggactg tgcggataaa cgcttattga aaaggtgaat aggcaaataa ttaatatgcc
     1621 ctatttctcc tcttgctctt tagccagtat ggcttccctt tttttgttgc cttcattttt
     1681 tgcaggaatt gaacgattgc tttgttggct gccatgactt tgctgattcg cgttagattt
     1741 ttttcgttgt tgatgtgtgc tggctgcagc atttgtattt cctgatgaag aacgttgctg
     1801 gcctcctcct gaacgacgtc tacgatttgc gttgctgcct tgttgacgtt tgtctcctcc
     1861 tgagcttttg gacgatgcgt gtttaccttg attttgagca cgatgatgtt ttttaccggg
     1921 ttcttgagtt acggtgtggg caactgaatg accaccaaat aaactggtcc ataatcgttt
     1981 aataaaactg gtttgagggt gtttgatgac cctgtgttcg gcaaatgatt tcacagcagg
     2041 ttcatcatgt gacacgggtt ctttatcact gctgggaaca tgtacttcag gttgctgaat
     2101 caaagagtaa cttggttttt tagatttgcc tacgttgtct tctttcaggc gagtaatcgt
     2161 atattgtgga gggtgcatat aaggattcgc aataacaaca acactgacac catgcctttt
     2221 ttcaatgtcc ctgataaaat tacgtttttc attcataatg aatgtggcca tttctggtgg
     2281 caattgcact tgaatctcag cagttttttc cttgagcgct tcttcttcga taaggcgggt
     2341 aattgataag ccatgagact gtatactgcg tacagttcct ctaccttcac aacgtggaca
     2401 aatttcctgg gcagtttctc ctaatgataa tcggaggcgt tgccttgaca tttcaagaag
     2461 gccaaagcgt gaaattcgcc ctacttgaat tctggcccta tcagcctgaa gtgcttcttt
     2521 gagtcgattt tctacctctc tttgattttt actagaactc atgtcaataa aatcaataac
     2581 taccaaacca cctaaatcac gcaggcgaag ctggcgagca atttcatcag ctgcttcaag
     2641 gttggtattt aatgcagtcg cttcaatgtc ggcaccgcct gtagccttgg ctgagttaat
     2701 atcaattgat accagagctt cagttctatc tatgaccaag gcacctccag aaggaagcat
     2761 gacctgacgc tgataagcgg tctcaatttg gctttcaatt tgatagaaat taaaaagagg
     2821 gatgctgctg ttatagagtt ttagattagg taaaaattct ggttttacgc gttcaatgta
     2881 ttgttttgct ttgatatagg atatttgatc atcaataata atttcactga tggattttcg
     2941 caaattgtca cgaatggaac gaataatgac atcgccctct tgatgaatca ggcaaggtgc
     3001 cagttctgaa ttataagctt ccttgatgga ttgccactga ttgcaaagca tgtttaagtc
     3061 atcttgcagt tcttccagac tttttccaac acctgcagtg cgaataataa ggcccatgtc
     3121 ttcagggagg gttaaagcat ttaatgtttc gcgtaattcg tcacgctcat caccttcaat
     3181 acgtcttgat attccgcccg aatgagggtt attgggcatt aaaaccaggt agcaacctgc
     3241 cagggtaata aaagttgtca gtgctgctcc tttattccct ctttcttctt tatccacttg
     3301 gataagtaat tcttgacctt cacggatgag gctggtaatt ggaggtttct catctccaaa
     3361 ctcttccgga ttcttgctta aatattcagg cgctatttct tttaggggta agaaaccttg
     3421 gcgtttggaa ccatattcaa cgaaaacagc atcaaggctg ggttctcttc ttgttacaat
     3481 ggctttataa atattacctt ttttctttat ttctcccggg cattcaatgt ccagatcaaa
     3541 taattgattg tttttaatta gtgcaacacg aatttcttct gtttgcattg cattgattag
     3601 cattttttcc atgtataacc ccatatttag ggcgcatgca tttacatcga gatagtgtaa
     3661 ggctcagtat agctatatga tcatttctac agttttcttg ccatgaagtt aattttccgg
     3721 gtcccacatc ttgcattcgt cttgaatctg cagagagagg gagtcagaaa attaatatcc
     3781 tggtctctat aattctggtg aaatgataac agcctattcc ctaattgctc aattttgttt
     3841 ttgaaaaaca atgttattaa gctggcaggg tgaggaactt taagctgacc catgaccttg
     3901 tggacagacc gtacgcatgt tacctcagta gttaaaggtg cacactgtat gatgtaccgt
     3961 ttacagatta agtgggttgg aaaaaaaagg cctttcatca ggcgtgcaaa agcccggcaa
     4021 taattcgtaa ttggtataaa gcatgtctgg catgcgtagg tatctgtcga aataattatc
     4081 aattttactc tggtattggg tcgcttcctt atatcttcga cttaaatgga agcgcatttt
     4141 gttttttctt ccaagcatct actcaggctt attacgatga aaaaaacaga ggtgattctt
     4201 taacttgtgg ttcaagctct tattgaattc aacctgtttt atattcgctt gagcagctgc
     4261 ttctttagtt cgatttgcta tcaaattaac taaatttatg ctattctgtt aatttttcat
     4321 tcttcattaa tgatgaaaaa ttaagatgaa cgcaagtatt ttaactaatt aattacaaat
     4381 cttttatcga ttatgaatcg attatt
//
LOCUS       DASLOE010000204         4403 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.205, whole genome shotgun sequence.
ACCESSION   DASLOE010000204 DASLOE010000000
VERSION     DASLOE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.205"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..359
                     /locus_tag="U6309_002684"
     CDS             <1..359
                     /locus_tag="U6309_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YHS domain protein"
                     /protein_id="HEN5506099.1"
                     /translation="QGYDPVSYFQQGGPVRGSGNYTGYYEGVSYIFSNQENKKTFLAN
                     PEKYLPAYGGYCAYGVSVGSKIVSDPLAWRIVDGTLYLNLNSKTQDVWAKNIPGNIKK
                     ANINWPKIKGIDPKDL"
     gene            664..1185
                     /locus_tag="U6309_002685"
     CDS             664..1185
                     /locus_tag="U6309_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506100.1"
                     /translation="MKEKKRSKTSKYLSNLVLILALSMMVSSAVISYFFIRQNELLIS
                     SLKMQAQNKQILIKDVWNNIVKKETRADIAILLSVLPVKDEVEVQAIKKDYLRDFSEL
                     TGTSSTADIVKEVKKEIERYGEYIDNLYLDQVTIQNKATAIEGSNKLYSEIAFFLQIF
                     SVVLIILRKDFTE"
     gene            1375..1668
                     /locus_tag="U6309_002686"
     CDS             1375..1668
                     /locus_tag="U6309_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEN5506101.1"
                     /translation="MIKNGIFTAVAAAITLTTSVAFAEGEMEKCSVVKDGKGLIKENK
                     ADCKGSSHSCAGQNKAGDSESWILVPKGQCDKINAGDFSAVGQDIKDKIEVSN"
     gene            1683..2537
                     /locus_tag="U6309_002687"
     CDS             1683..2537
                     /locus_tag="U6309_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEN5506102.1"
                     /translation="MTLNPLIGIGLRYPHYTQVLEERPSIGWFEVHSENFFSNGGNEI
                     KALLNISEYYPISLHGVGLSLGSSDGISKVHLDRLSNLVHQVQPKFVSEHLSWGHVGG
                     IHMPDLLPVPYTEESFNTFKRSISIAQDFLKREILIENPSSYIEYKSSRLHESYFLVE
                     LCNQTGAKILLDVNNLFISSWNHGWNTKEYIDSIPFNLVKEIHIAGHSIKELSNDSTL
                     RIDTHNNYVCDEVWALYDYTVQRFGPIPTLLEWDADIPSLNVLIEEASKCESYLSSYK
                     VNRGVANA"
     gene            2530..3309
                     /locus_tag="U6309_002688"
     CDS             2530..3309
                     /locus_tag="U6309_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HEN5506103.1"
                     /translation="MHELLKLQESFYRNIFKKDVDLSFISSDFSQERLDVYRQTIFEN
                     MINALRITYPGVWKLIGNECANSVAYTYCKKDKYLPKTGWLDDFGGDFPDFLSTLQQL
                     SELPYLSDYAHYEWLKHLAYGAVDSKSISPQDLMTIPEEEIDHIKLNFCPSVCIFQSK
                     YPLFDIHDTIENCSSKAITLKTEAAYGVIGCKENEIHTYWIAEDQWHFIKKLFEGAKL
                     LESVQYAQTINQNFDLTSAVAFVLQEQLVDNIIKTGGNNAQ"
     gene            3299..3796
                     /locus_tag="U6309_002689"
     CDS             3299..3796
                     /locus_tag="U6309_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEN5506104.1"
                     /translation="MLNNSVETSINKPFISKIIQIYLSVTKVLEKIALPVLVLFMRLW
                     MAKIFWYSGMTKISNWQSTVFLFREEYKVPFVPPEITACLATSFELACPVLLIFGFAT
                     RLATLPLLAMTAVIQFTYLDLIDHRYWAMLLALILFYGPGSLSLDHLISKKFGLLASS
                     RGDVA"
     gene            complement(3966..4142)
                     /locus_tag="U6309_002690"
     CDS             complement(3966..4142)
                     /locus_tag="U6309_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEN5506105.1"
                     /translation="MLCTEANQAHWVILFILALYFILGRILHAYAFIYNKKHFKFRVR
                     GMVLTFSALFSLRC"
BASE COUNT         1417 a          752 c          838 g         1396 t
ORIGIN      
        1 ttcaggggta tgatcctgtg tcttattttc aacaaggtgg gcctgtacgt ggtagcggaa
       61 actacacggg atattatgaa ggtgtgagtt atattttttc taatcaagag aacaaaaaaa
      121 catttcttgc aaatcctgaa aaatatcttc ccgcttatgg aggatattgc gcgtatggcg
      181 tatccgttgg gagcaaaata gtgagtgatc ctttagcttg gagaattgtg gatggaacac
      241 tctatctaaa tttaaacagc aaaacacaag atgtttgggc aaaaaatata cctggcaaca
      301 taaagaaggc aaatattaat tggccaaaaa ttaaaggcat cgaccctaaa gacttataat
      361 aagttgcact gttttaatat aattctcctt cccacttagt ttgcatccgg atttgttttg
      421 cggttcgaat taagaagttt ggaaggggaa atgatgtaat gagtatgtat tcattaagta
      481 aagattcggg aaattgtggt gattaataca gactctatag cctttaaaca gactaccgtc
      541 agcggtaagc cagtaatcgt ggctgggaaa gccttgacat cttttaatat tcacctaaac
      601 ttttcattaa aacgttgagc ttaatatatc gataatatca tgttcattag ctgaagaagg
      661 aatatgaaag aaaaaaaaag gagtaaaaca agtaaatacc taagtaattt agttttaatt
      721 cttgctctct ccatgatggt tagctcagct gtgatttcat atttctttat cagacaaaat
      781 gagctgctta tatcttcttt gaaaatgcaa gcgcagaaca aacaaattct cattaaagat
      841 gtatggaaca atatagtaaa aaaagaaacc agagctgata tcgcgatact tctgtctgtt
      901 ttacctgtaa aagatgaggt cgaagttcag gccattaaaa aagactattt gagagacttt
      961 tctgagttaa caggaacatc aagtacagcg gatattgtca aagaggttaa aaaggaaatt
     1021 gagcgttatg gtgaatatat tgataattta tatcttgatc aagtgactat tcaaaataaa
     1081 gcaactgcaa tcgaaggtag taataaattg tattctgaaa tagcgttttt tctacaaata
     1141 tttagcgttg tattgattat tttaaggaag gattttacag aataataata gtctcaacga
     1201 tggatcttaa tcccaagtta ctccttaaca cgaaagttta taaacttgtt taaccttgac
     1261 agcgaattga tcggtgctat atttaaaaga taacatatcg gatagtcctg aaataatatg
     1321 attgttttcc aataccatgt tatgtaactg ttatattaat caaggggcat taaaatgata
     1381 aaaaacggaa tttttacagc agttgcagca gcgataactt taacaactag tgttgcattt
     1441 gctgaaggtg aaatggagaa atgtagcgtt gttaaagatg gcaagggatt aattaaggaa
     1501 aacaaagcag attgcaaagg ttcttcccac tcctgtgctg gacaaaataa agcaggtgat
     1561 tctgaatcgt ggatcttagt accaaaaggt caatgtgata aaataaatgc gggtgatttt
     1621 agcgctgttg ggcaagacat taaagataaa atcgaagttt caaattaatc gattcgtata
     1681 ttatgacttt gaacccgcta ataggtattg gacttcggta ccctcattac acacaagttc
     1741 tagaggagcg accttcgatt ggctggtttg aagttcatag cgaaaatttc ttctccaacg
     1801 gcggaaatga gataaaagct cttttaaata tttccgaata ttatccgata agccttcatg
     1861 gtgttgggct atctttgggt tcatccgatg gtatctccaa agtccatttg gatcgcctat
     1921 cgaatcttgt tcatcaggtg cagcctaaat ttgtttctga gcatctttca tggggacatg
     1981 ttggtggcat acacatgcct gatttacttc cagtgcctta tacagaagaa agttttaaca
     2041 cctttaagcg cagtatatcg atagcacagg attttttaaa acgagaaatt cttattgaga
     2101 acccatcttc ctatatagaa tataagtcat ccaggctcca tgaatcctat tttttggtgg
     2161 agctttgcaa tcaaacaggt gccaagattc tgctcgatgt gaataaccta ttcatctcat
     2221 cctggaatca cggatggaat accaaagagt acattgattc tattccattt aatttagtaa
     2281 aagaaattca tattgctggt cattccataa aagaattgtc aaacgatagt actctgcgta
     2341 tagatactca caataattat gtgtgtgatg aggtgtgggc tctatacgat tatactgttc
     2401 aaagatttgg ccctatacca actttacttg aatgggatgc agacatccct tctctcaatg
     2461 ttttaattga ggaagcttca aaatgcgagt catacctctc gtcttacaaa gtcaataggg
     2521 gggtagcaaa tgcatgaatt acttaaatta caagagtcct tttatcgaaa tatctttaaa
     2581 aaagacgttg atttaagttt tataagctcg gatttctccc aagaacggct agatgtttat
     2641 cgtcagacta tttttgaaaa tatgatcaat gctttgagga ttacttatcc aggcgtttgg
     2701 aaattaatcg gcaatgagtg tgccaatagt gttgcctata cttattgcaa aaaggataag
     2761 tacttaccaa agacaggctg gcttgatgat tttggtggag atttccctga ctttctttcc
     2821 acattacagc aactgtctga gttgccctat ttaagtgatt atgcccacta tgaatggctc
     2881 aaacatttgg cgtatggggc tgtagactct aagtccattt cccctcaaga tttaatgacc
     2941 atccccgaag aggaaataga tcatatcaaa ctgaattttt gtccttcagt ttgtattttt
     3001 cagtccaaat acccgttgtt tgatattcat gataccattg agaattgtag ttcaaaagca
     3061 attaccctta agacagaagc agcttatggt gttattggct gtaaggaaaa cgaaatacat
     3121 acctattgga ttgccgaaga tcagtggcat ttcataaaga aattatttga aggtgcaaag
     3181 cttttagaga gcgtccagta tgcacaaaca atcaatcaaa attttgactt aacttcagca
     3241 gttgcttttg tgttacagga gcagctggtc gacaatatca ttaaaaccgg aggcaataat
     3301 gctcaataat tcggtagaaa cttctataaa taagccgttc atttctaaaa ttattcaaat
     3361 ttatctatca gttacaaagg ttttggagaa aattgccctt cctgtgcttg ttttattcat
     3421 gcgcttatgg atggctaaaa tcttctggta ttctggcatg actaaaatat caaactggca
     3481 gtcgaccgtc ttcttgttta gggaagaata taaagttcct tttgtgcctc cagaaattac
     3541 tgcttgtctt gcaacctctt ttgagctcgc ttgccctgta cttcttatct ttgggtttgc
     3601 aacaagactt gcaacattgc ctttactggc tatgaccgct gtcattcagt ttacctatct
     3661 cgatttaata gaccatcgtt actgggctat gctgcttgct ttgattttat tttatggccc
     3721 gggttcacta tctcttgacc acttaatttc taagaaattt ggattattgg catcttcgcg
     3781 tggcgatgtt gcctaaatca aatttggtgt gcataatgct tctgagtata ataagtccag
     3841 cgatctcaat aattcttttt ctaatagatt gcccgctcag aaaaagtaaa tactttgaac
     3901 agatttatgc agggtaaaaa aataattgtt ttatcacccg agtttaaaaa gtattaaact
     3961 aatgttcaac accgcaagga aaaaagtgca gaaaacgtca gaaccattcc ccgcacacga
     4021 aatttgaaat gctttttatt ataaataaaa gcataagcat gaagaattct tcccagaata
     4081 aaatataagg ccaagatgaa tagaattacc caatgggctt gatttgcttc agtgcaaagc
     4141 atcaatatca atgcaacccg tatttaggcg aacgcttacg tatatctgcc tgtatttaaa
     4201 acttatatgg gacacgactc ttttcacgaa agggcctatt accttcgtat gacgatcgat
     4261 gtgtttcccg atataaagtt gaaaatggag actaggtacc caggaataat ccctgatttg
     4321 caaggcgata acgatgaaac caactgattt tgcagaatat tttactgaat ttcttactgt
     4381 ttatttaccc aaacaaaaaa atg
//
LOCUS       DASLOE010000205         4390 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.206, whole genome shotgun sequence.
ACCESSION   DASLOE010000205 DASLOE010000000
VERSION     DASLOE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.206"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            212..1864
                     /gene="kaiC"
                     /locus_tag="U6309_002691"
     CDS             212..1864
                     /gene="kaiC"
                     /locus_tag="U6309_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEN5506106.1"
                     /translation="MNINKTKTGIKGLDDILNGGYLKNKPTLLKGSPGTGKTIFTLFF
                     THEQLKSNNSVIYVTCDESPEQIIAHMDGFNLESSKFLQEGKLLILDFTPILTDEVAG
                     EFNINALLLRIEQAQQKIKANTLIIDSVQSILLGIKNYDPNYELLRLFQWARQKNLTV
                     LTTMAETQTILQTEIYDEYVVDCAIELKQKVNNDLMTRYLRIIKNRGSSHGTNEYPFS
                     ITHKGISLLPITSTKLNTTIPAEYLRTGIKVLDRMLDNKGYQTGSQIIISGRSGTAKT
                     LFAASFAQSAVRQGRKVLFISFEESPNDLIHHVKSINIDLMKDITKKKLHIDSRRSVE
                     MGLEDHIISIIELTEEKQFDVLILDPISSLLDLGNTMEVKRLFIRFISYMKSLNKTLL
                     FTELIPDYSKELTILGLSSLTETWIRLSNVENNGEFNRLIHIAKARGIKTSNQIKEFY
                     VTDKGINIEAPYLGDNQMMFGSQKSAHILREKQELQQRKEEIKRLEREITNLTEIQRA
                     QLKIQEANFIAKRSELIAKKNELITKEEALIKRMESNKLLRE"
     gene            1869..2141
                     /locus_tag="U6309_002692"
     CDS             1869..2141
                     /locus_tag="U6309_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEN5506107.1"
                     /translation="MTRTKLKLFVIGNSAISKRAIINLQSICSDPKLADLCDIEVVDL
                     CKNKGVAEQEKILATPILIKKEPLPERRIIGDLSDKQKVISALEMD"
     gene            2207..4390
                     /locus_tag="U6309_002693"
                     /pseudo
     CDS             2207..4390
                     /locus_tag="U6309_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213470.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT         1565 a          822 c          768 g         1235 t
ORIGIN      
        1 ctgtattctt tatttaaaca atccaactca aaacccatca tagaatgatt cggatagttc
       61 taaatacaac taaatacaaa acttattacg acaaaacttg ttacgtctct atcaagacga
      121 aatattagat ctataattaa acaatcagca tattatttac cgcttcaaca atcgtatttt
      181 aaagatatgc atctgctgag aggacgaaaa tgtgaatatc aataaaacaa aaacgggaat
      241 taagggatta gatgatattt taaatggtgg ttatttaaaa aataaaccaa cccttctcaa
      301 aggcagccct ggtacaggaa aaacaatatt tacccttttt tttacgcatg aacaacttaa
      361 gtcaaacaac agcgtcattt atgtaacctg cgatgagtca cctgagcaaa ttattgctca
      421 tatggatggc tttaacctgg aaagcagcaa atttttacaa gaaggcaagc ttttaattct
      481 tgactttaca cctatattaa cggacgaagt ggccggggaa ttcaacatca atgcactttt
      541 actccgtata gaacaggcac aacagaaaat caaagccaat acactgatta ttgattcagt
      601 tcagtccatt cttttaggca tcaaaaatta tgatcccaac tacgagttgt tgagattgtt
      661 tcagtgggcg cggcaaaaaa atcttaccgt tctcacaacg atggccgaaa cccaaacgat
      721 tttacaaaca gaaatctatg acgaatatgt tgttgattgt gctattgagc taaaacaaaa
      781 agtcaataac gacctgatga cgcgctattt gcgaatcatt aaaaatcgtg gttcgtctca
      841 tgggactaat gagtatccct tttctattac tcataagggc atttccttgc ttcccataac
      901 cagcacaaaa ctgaatacaa caattcccgc tgaatatctc cgcactggca taaaagtcct
      961 ggataggatg ttagacaata aagggtacca aacaggatcg caaataataa tcagcggccg
     1021 ctccggaaca gcaaaaacac tttttgcagc aagtttcgcc caatccgctg ttcgacaagg
     1081 caggaaagtg ctgttcatct catttgagga atccccaaat gatttaattc atcatgtcaa
     1141 gtccatcaat attgatttaa tgaaagacat caccaaaaaa aaattacaca ttgattcaag
     1201 acgctctgtg gaaatgggac tggaagacca tattatttca atcatcgaat taacagaaga
     1261 aaagcaattt gatgtcctga tactggatcc catatcatct ctgctggatt tgggtaatac
     1321 gatggaggtc aagagattgt tcatacgttt tatttcttat atgaaatctc tcaataaaac
     1381 tcttttattt actgaattga tacctgatta ctccaaagag ctaaccatac tgggcttatc
     1441 cagcttaaca gaaacctgga ttcgcttatc caatgtggaa aacaatggag aattcaatcg
     1501 attaatccac attgccaagg cgcgtggcat aaaaacatcc aaccaaatca aagaatttta
     1561 tgtcacggat aaagggatta acatcgaagc cccttatctc ggtgataacc agatgatgtt
     1621 tggttctcaa aaatcagccc acatcttgag agaaaaacaa gaattacaac agaggaaaga
     1681 agaaattaaa cgcctagaac gtgaaatcac taatcttaca gaaatacaac gtgctcagct
     1741 caaaattcaa gaagcaaact ttatagctaa aaggagtgaa ttgatagcta aaaaaaacga
     1801 gttgattact aaagaagaag cgctgattaa acgaatggag tccaacaaat tgttgagaga
     1861 gtaaatgaat gactcgaact aagctaaaac tgtttgtaat tggaaatagt gccatatcaa
     1921 aacgtgccat tattaatttg caatcgatat gctcagatcc caagcttgct gatttgtgcg
     1981 atatagaggt agtcgatcta tgtaaaaaca agggggtagc tgaacaagaa aaaattctgg
     2041 ccactccaat attaattaaa aaagaaccct tgcctgaacg tcgaatcatt ggtgatttat
     2101 ccgataagca aaaagttatt tctgctttgg aaatggatta ggtgatatcg tgacatcaaa
     2161 cgagcatata aacacacaaa acttttggta ccaactcgtt gaaaaaatgg ctattggggt
     2221 attggtgatt aataatgaag ggcaaattct ttacgcgaat gattatgtag tacaaatgtt
     2281 tgatgtaccc catccagaac ttgttggcac caatttttta tatccgatgg cagcaaatga
     2341 aacacaggaa gttgaaattt taaaatccag taatgaaatt ttaacagcac atatgacagt
     2401 caggaaaagt atctgggaaa atcgccaggt atggattatt tctctctttg atattacaga
     2461 gctcaaacta aaagaaaaac ttttaaaaat ttcaagcaaa ggcatcagtt cagcgtttga
     2521 aggtattatc atcaccaatc cggaaggcac cattattgaa accaataacg catttatgaa
     2581 aatgacagga tatacagagc aggaagtcat aggccaaaaa cctgctttct tgcattcaaa
     2641 gaagcaaaac gagaaatttt acaaggagct atggaatata ctcatagcga aaggtcagtg
     2701 gagcggtgaa ctttgggcaa aaaagaaaaa taaaaggcta tttcctgcat ttatggccat
     2761 ctcagccgtt aaaaattccg atggtgatat catcaactac attggttatt atcatgatct
     2821 cacgttaata aaaaaacaag aacaacaaat tgagaatttc aaatattatg attatttaac
     2881 aggattaccg aataaacttt tactcaccca aagacttcag atgcttatta acagattatc
     2941 tgaagaaaat aaagaactca tcatttttaa tattaggata tttgattcga atagcaaaaa
     3001 ctctctttta gatttacccc caatactcgc gataaaatca tcttggaggt ggttaagcgt
     3061 attgatcgaa catcgcctaa aaaaatgttt ttctcacgca tcggcttaag tgagtttatt
     3121 gttgtttatt ttatcaaaag tagaattgaa gtgatggctt ccatcgctca aagaatgata
     3181 gaaaaactct cttgccctta tcacattagt cacaaaacta ttatcttgca aacgatcatt
     3241 ggactaactg cgtactacaa aaataccgcg ttcagcgccg aggatttttt gcaacaatca
     3301 gaagttgccc gccacagagc acaagtcagg ggtattaata tttttgagct ttttgaccct
     3361 caggttgaag aacgaattat agaatttaat aaacgcattg aatctgtctc taaagcaatc
     3421 atgttaaatg agttgaaaat ttattaccaa cctattgtgg aattaagtac agctgaagtg
     3481 gcaggtttgg aagccttgct gcgctggaaa caccccaaaa aaggcctgtt acttccaaaa
     3541 caatttttat ctgacatcga taatcacccc ctttctctga aacttggaga ttgggttctg
     3601 catcaggtat tagagcaaat tgaaaaatta ctggcttgca acataaaaat ccctattcac
     3661 attaatgtga gcgcttatca gctgcaacat cccaattttg tcaaaaaact gtttgacaca
     3721 ttggcactat acccaaacgt tccccatcac ctaatcatgc ttgaaatcct cgaaacacaa
     3781 gccattgaag atttgatatt agtatcccag ataatagccg agtgtaagac taagggagtc
     3841 aacttttctt tggatgattt tggaacaggt tacgcctctt taacttttct taaagaattg
     3901 aataccactg aagttaaaat tgatcgaagt tttatcattg atatttttga taacccgaag
     3961 gaacttgtta ttctgaaaac aatgatagaa ttttgccgag tcattggtcg taccctggtt
     4021 gcagaagggg ttgaaacagt agcacatggg aaattattaa gcttgtttgg ttgcaatcta
     4081 atccaaggct acgctattgc caaacctatc cccgctacca aattaattcc atggataaaa
     4141 aactggaagc ttgctccaga atggggagaa aaccggtttt cgcatgccgc attaaataaa
     4201 ttagtcaaaa tctccataaa acactatctt aactttcaat taatcagact ttatttagag
     4261 aatgatcaat ctcccaaacc tcaattatca cttcaagcat gcccagtaga gcagtggttt
     4321 cttgaaaatc aacaaacaat caaagaggac aggcgattta aaaaagtctg tgatttacat
     4381 cacaaggaac
//
LOCUS       DASLOE010000206         4358 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.207, whole genome shotgun sequence.
ACCESSION   DASLOE010000206 DASLOE010000000
VERSION     DASLOE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.207"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(32..406)
                     /locus_tag="U6309_002694"
     CDS             complement(32..406)
                     /locus_tag="U6309_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506108.1"
                     /translation="MHQPDIQHNRKRGYIMARITVTLPDNLHKQIIKIAGKENDSLSY
                     TTTRLVEIGLMVMNSKSDNKDEQKTTNIEEYCQKLIIQINGIIKEIAVDKFNFDDDKI
                     VQITKDTLNKFNKLKGIQQESL"
     gene            1254..2774
                     /gene="ltrA"
                     /locus_tag="U6309_002695"
     CDS             1254..2774
                     /gene="ltrA"
                     /locus_tag="U6309_002695"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN5506109.1"
                     /translation="MTDNLLSGAPDAFILNTKWDQLDWPSIEKNVYRLQVRIAKAVSN
                     KQHGKVKSLQWLLVNSISAKLLAVRRVTTAKGSKTPGIDGVVWTTSEEKCEAVRNLKA
                     RGYKATPLRRIYIPKKNGKERPLSIPTLKDRAMQALYLLALEPVGETTADLNSYGFRP
                     KRSTHDAIYQCYATLARKNCAQWILEGDIKACFDEIDHGWLKSNIIIDQRVLTQWLQA
                     GYMEKNQLFETARGTPQGGPASPLLANMVLDGLEREIHSGCGQGNKINYIRFADDFIV
                     TANSPDILKEKVMPIISNFLAQRGLSLSQEKTKIVHIEEGFDFLGFNVRKYKGKFLTT
                     PSKDSIKSVQMKIKETVKKGYGWKGSELISALNPIIKGWANYHRKVVSKATFSELDNY
                     IYQETFRWTMRKFSGHNRYKAMDRYFRNRSLTRRWIFSDVVKAKDGTKKYVCINKMMD
                     IKIQRHVKIRSSANPYLPEYVKYFEDRKKWAKDCSFVQRIKDKQLNVIGSEYLLGE"
     gene            complement(2845..4296)
                     /locus_tag="U6309_002696"
     CDS             complement(2845..4296)
                     /locus_tag="U6309_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISLpn5 family transposase"
                     /protein_id="HEN5506110.1"
                     /translation="MIKGNQNNSSSGWAESEFGIVNFGDKRLSKRLLKIADSFANSPE
                     CSINRACDDWHQSKAAYRFFQNDAVSERKILDSHITKTIERAKNYPTILAIQDTSYIS
                     YKNHKKTEGLGIIAARVRSKTTNFQTHGLVMHTTFAVTTEGLPIGLLDQKISSRPLLD
                     ETVKELKKRSHNIALPIEEKESMRWIESLEHSNNYPDLKNAKVVTVCDREADIYDLFE
                     VASTNQCPFVVRARQDRTVNKTSIYSKKSGEKLWDLVSGSPCRGEIQVTIPARDNKPK
                     RTATLEVRFDHFVMNPPKNNVKRKTRTLPDLKLNAVYVIEQSPPLGEEPMNWMLLTNI
                     DINNFEEAVEKIQWYCLRWRIEIFHKILKSGFKVEECRLGAADRLVRFLTIMSVIAWR
                     IFFITLVARESPDLPCSFLLANEEWNVLYTKIHHTKHYPETPPTIKEAVKWIAQLGGF
                     LARKNDGEPGPITLWRGWKRLVDLAEGWNLALS"
BASE COUNT         1358 a          822 c          869 g         1309 t
ORIGIN      
        1 agtatgttaa aagtctaagg gcaagataaa gctatagtga ttcctgttga atacctttta
       61 atttattaaa tttgtttaag gtatctttgg ttatttgaac gattttatca tcatcaaaat
      121 taaatttatc aacggcaatt tctttgatga tgccattaat ttgaataatg agtttttggc
      181 aatactcttc aatgtttgta gtcttctgct catctttatt atcagactta ctgttcatca
      241 ccatcagtcc aatttcaaca agccgtgttg tcgtataaga gagtgaatca ttctccttac
      301 cggctatttt aattatttgt ttatgaagat tgtcaggtaa ggtcacggtg attctagcca
      361 taatataacc tctttttcga ttgtgttgga tgtctggctg atgcatccat gcacgccacc
      421 cctaattaca attagatcat attaggctat atatttcaac tggttacgag tgtcagtatg
      481 cagatctgca tcagataaat ctgacaaaat atttaaaagt gatgcacatt tgcatactgg
      541 tttttgattg ctatttagca aatatttagt gtttcgtagc cgattcttgc aaatatgatt
      601 ttatattgaa taaatacaat gagttacaca cacagcaacc agttgcgaaa ggtgtggtgc
      661 gacaagataa gtcgctgttt tgagttgtta atcggaagga atttcgaaaa ccatttgtat
      721 cgtcagatga acctacggga gttaataaag cctccctgac aatgtagtga aactacagct
      781 gaagggctga tagtgagatg ttaacatccg aaaggaagta aatctacctg atagccaaaa
      841 tcgggatagc tactaggtag ctggtatggt gaacataagt gaatctgtga taaaggtcgt
      901 aactcgcaac aagcgcaatt ggctgaatcg cttgaaccaa aaggtacgaa aggtagctgg
      961 ggaaatcaca cgaaccccac acgattacga aactttccgg ccgagagcag acacctaacc
     1021 cggcattact tgaacagcgg aacttggaaa ccctatattt ctcttcgtaa gaagacagta
     1081 gcctgcaaag gctgcctatg gaagtgtagg cttgggatat cggaaaaagc gaaggccaat
     1141 ttgtaatgag ttggatacgg gtttcaatgt cacccggtct gaaaagaagc ccacgtccga
     1201 ttggtctttc attgcgaaag aatttgcaga atttgtagaa gtgaggaaag caaatgacgg
     1261 ataatttatt atctggtgcg cctgacgcat tcatcttaaa tacaaagtgg gatcagctcg
     1321 actggcctag tattgagaaa aatgtataca gacttcaagt gcgtatcgca aaggcagtga
     1381 gtaataaaca gcacggaaaa gtgaagtcat tgcaatggct gctggttaat tccatcagcg
     1441 caaaacttct tgcagtgaga cgagtaacga cagcaaaagg aagcaagacg cctggtattg
     1501 atggtgttgt ttggacaaca tcagaagaaa aatgcgaggc ggtgcgcaac ctaaaagcaa
     1561 gagggtataa ggcaactccg cttcgaagga tttacatccc gaagaagaat ggaaaagagc
     1621 gtccactaag catacccact ttgaaagatc gggcaatgca agcactctat ttgttagcat
     1681 tagaacccgt tggagagact acagctgatc taaattccta tggatttaga ccaaagcgaa
     1741 gtactcatga cgcgatatac cagtgctatg caaccttggc acggaaaaat tgcgctcaat
     1801 ggattttaga aggcgacatt aaggcctgtt ttgatgaaat tgatcatgga tggctcaaaa
     1861 gtaatatcat aatagatcaa cgagtgctaa cacagtggtt gcaggcaggc tacatggaaa
     1921 agaatcaatt atttgaaaca gcacgaggta ctccgcaagg tggtccagcc tcaccactgc
     1981 tcgcaaatat ggttctagac ggacttgaga gggaaattca ctccggctgt gggcaaggca
     2041 ataaaataaa ttatattcgt tttgccgatg atttcatcgt aactgcaaac agtcctgata
     2101 tcctcaaaga aaaagtgatg ccgataattt caaactttct tgcacaacga ggtctttcat
     2161 tgtcacagga aaaaaccaaa attgtgcata tcgaggaagg ctttgatttt ctcggattta
     2221 atgtcaggaa atataagggt aagttcttaa ctacgccctc aaaagacagc attaaatccg
     2281 tacaaatgaa aatcaaagag accgtcaaga aaggatatgg ttggaaaggc tcggagctta
     2341 tctcagcgct gaatcctatc ataaaaggat gggctaacta tcatcgaaaa gtcgtctcaa
     2401 aagcaacatt ttccgaactg gataattaca tatatcaaga aacattccgt tggacgatga
     2461 ggaagtttag tggtcataat cgctataaag caatggatcg ttattttcga aatcgtagcc
     2521 ttacaaggcg ctggattttt agtgacgtgg ttaaggccaa ggacggtacg aagaagtatg
     2581 tttgtattaa caaaatgatg gatataaaaa tacaacgaca cgtcaaaatt aggtcgagcg
     2641 ccaaccccta tctacccgag tatgttaaat actttgaaga tagaaaaaaa tgggctaaag
     2701 actgctcctt tgttcaacga ataaaggaca aacaattgaa tgttattggt agcgaatacc
     2761 tgctgggtga atagcttcag aaaggcttga gcgtagtgcg gtgaaagtcg catgctgcgt
     2821 tcttaggctt actattaccc ataactaaga aagagcaaga ttccaacctt ccgcaaggtc
     2881 gactaatctt ttccatcctc tccacaatgt tataggacca ggttccccat cgtttttacg
     2941 ggctaaaaag ccgcctagct gagctatcca tttcacggct tcttttattg tgggtggtgt
     3001 ttcaggatag tgttttgtat gatgtatttt ggtatacaag acgttccatt cctcattagc
     3061 caataagaac gaacaaggaa gatcggggct ttctctagca actagtgtga taaaaaaaat
     3121 tcgccatgca atgacactca ttattgtaag aaagcgaact aatctatcgg ctgcaccgag
     3181 cctgcattct tcaactttaa aaccagattt taagattttg tgaaaaattt ctatcctcca
     3241 tcttaagcaa taccattgta ttttttctac tgcctcttca aaattattaa tgtctatgtt
     3301 ggtcaacaac atccaattca taggctcttc gcccagagga ggagattgtt ctatgacata
     3361 cactgcattt agttttaaat cagggagtgt tctggtttta cgtttcacgt tatttttagg
     3421 agggttcatc acaaaatgat caaaccttac ttctaatgtt gctgttcttt taggcttatt
     3481 atctcgagca ggaatagtga cttgaatttc cccccgacaa ggcgaaccac tcaccaaatc
     3541 ccataatttt tcaccacttt ttttagagta aattgatgtc ttatttactg ttctatcttg
     3601 acgtgccctc actacaaaag gacattgatt tgtcgatgct acttcaaata aatcataaat
     3661 atctgcctct ctatcacaaa cagtaaccac cttagcattt tttaaatccg gataattatt
     3721 agagtgttcg agagactcga tccaacgcat actttctttt tcctctatag gaagagcgat
     3781 attatgactt ctttttttca actcctttac tgtctcatct aaaagcggtc tagaactaat
     3841 tttttgatcc agtaatccta taggcaggcc ttctgtagtt actgcaaatg tggtatgcat
     3901 taccaatcca tgagtttgaa aattagttgt tttagagcga actctagctg ctataattcc
     3961 taatccttca gtctttttat gatttttata agaaatataa ctcgtatcct ggatggctaa
     4021 aatagttgga tagttcttgg ctctttcaat agttttagtg atatgactat caaggatttt
     4081 tctttccgat acagcatcat tttgaaaaaa tctataagct gctttacttt gatgccaatc
     4141 gtcacatgct cgatttattg aacattcagg ggagttggca aagctatctg ctatttttag
     4201 aagtctttta cttaatcgtt tatcaccaaa attaactatt ccgaactcac tctctgccca
     4261 tccagaagat gagttatttt gattcccttt tatcatttgc tctcattatc agtcaaaata
     4321 ctgcccttat ttataacaga gagttaaatt aaagaaaa
//
LOCUS       DASLOE010000207         4358 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.208, whole genome shotgun sequence.
ACCESSION   DASLOE010000207 DASLOE010000000
VERSION     DASLOE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.208"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            45..977
                     /locus_tag="U6309_002697"
     CDS             45..977
                     /locus_tag="U6309_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEN5506111.1"
                     /translation="MKRCPITYEKISDQENYSQRGLRLLSPQLKNLSPLDLSADEQRQ
                     EAIARVGKMSIQGVQKKLSAKLKIKEGSFEIVDQNGHYILKPQSDIYPELPENEAITM
                     TLAKTIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
                     SMEKVIAIIEQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITRDRKISISPAY
                     DLLNSTIAQKNTKEEIALPLKGKKNNLTKSDFLNYFAVEKLGLNQNVINGIAQEFHQA
                     IPEWRELISFSFLSQPMQEKYLQLLDQRCKRLNF"
     gene            1106..3673
                     /gene="mgtA"
                     /locus_tag="U6309_002698"
     CDS             1106..3673
                     /gene="mgtA"
                     /locus_tag="U6309_002698"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEN5506112.1"
                     /translation="MKINLDLNDLSTYDQDLLLNELNSSLEGLNNFQAQKRLTQYGTN
                     EILRKPYHLIIIEAISHSINPLVAILFFAALISAFTGNVVNAAIIISIIIISIGLDYF
                     QSHRALVAIKMLQKKIATTVTVLRENQWLEIPAKELVPGDLFRLSAGDMVPADSILLK
                     SKDLHIHQSALTGESMPVEKEAIPLKTKPKNPLDALNVVFSGSSVIGGSATALAINTG
                     SNSLYGHIAESLKKKPPHTEFEQGMMRFSTFIMKMVFLLVIFVFSINIYMHRPLLDSL
                     LFAVALAVGLTPELLPMITTVTLASGAVRMSKKKVIIKNLSAIQNFGSIDILCSDKTG
                     TLTSGEMNLTKYLDFSGKQSEFVMLLAYLNSVYITEIKSPFNIAVLKKARLNPLDLAI
                     LKHDHPDIQPYHKVDEIPFDFERRRSSVIVSKNENHLFICKGAPENIMSVCSYYDFAG
                     ERELFTEKEQKQCELLFQSLSSEGYRVLAIAYKLMDRQLKYTQSDEKEMIFAGFLAFT
                     DPLLEDIPKVIKDLRHEGINIKILSGDNLIVTQHICQKVGMDTSRILTGEEISKISDD
                     ALPPLAEQIDIYARINPMQKQRIISALKKRGHVVGYLGDGINDVPSLHQADVGISVAS
                     AVDIAREAADIVLLEHHLKVLLNGIIEGRKSFRNVMKYLMMGTSSNFGNMISMAIAVL
                     FLPFLPMLPRQILLNNLLYDISQITIPTDHVDPSSIHRYKRLDINIIRQFMFYVGPIS
                     SVFDFLTFFVMLKIFSANEALFQTGWFVESLATQTLVIFIIRTAKKPWQSMPSLPLTT
                     TVLLIVMVGIWLPFSPFSGLFGFVPLPPLYFLFLVTATFTYLFLVELIKKKIMRKLY"
     gene            3708..>4358
                     /locus_tag="U6309_002699"
     CDS             3708..>4358
                     /locus_tag="U6309_002699"
                     /inference="COORDINATES: protein motif:HMM:NF015651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEN5506113.1"
                     /translation="MIKWISGFIILVVLIIVIGHFSQLRVFLGLIEQMKFSWLFFAVI
                     FQSGTYMSLAAVWRLALRWFGEQISFFQLFLLSFSQLFVNQTIPTSGLSGVTIMTKFL
                     LGRLITPKAIALAIALNVFSRQVAYLTSFIASIVVFWFYNELNKTLISLSLIFILIMV
                     AVLILAALLWQQALKLKIPKLLNKYTSIRILINEIKKLDPKIVLNFKLFFPAVVFSV"
BASE COUNT         1432 a          772 c          749 g         1405 t
ORIGIN      
        1 gttggccagg atgtcgtagg cgcagtgact attgaggaaa taagatgaaa cgctgcccga
       61 ttacttatga aaaaatcagt gaccaagaaa actattcaca acgtggattg cgcttgcttt
      121 ctcctcaatt aaaaaacctg agcccattag atttaagtgc tgatgagcag cgtcaggaag
      181 caattgctcg agtaggaaag atgtctattc aaggtgtcca aaagaaacta agcgcaaaac
      241 taaagattaa agagggatct tttgaaatcg tggatcaaaa tggtcactat attttaaaac
      301 cacaaagtga tatttatcca gaattgccgg aaaacgaagc cattaccatg acccttgcaa
      361 agaccattgg ccttgaagtg cctgttcatg gtttagtgta ctctaaagat aacagtttga
      421 cttacttcat taaacggttt gatagaatag gccataataa aaaattagct ttagaagatt
      481 ttgcacagct atcgggtgaa gatcgacata caaaatataa aagttctatg gaaaaagtga
      541 ttgcaatcat agagcagttt tgcacgttcc ccaaaattga attcgtgaaa ttatttaagt
      601 tgacattgtt taacttcctg gtcggcaatg aagacatgca cctaaaaaat ttttctctaa
      661 ttactaggga tagaaaaatt tccatatcac cagcatacga tttacttaac tcaactatcg
      721 ctcaaaaaaa cacaaaagaa gaaatcgcat tgcccctcaa agggaaaaaa aataatttaa
      781 caaagagtga ttttcttaac tattttgccg ttgagaaatt aggattaaat cagaacgtca
      841 tcaatgggat cgcacaagag tttcatcagg caataccaga atggcgagag ttgattagtt
      901 tcagtttttt atctcaaccg atgcaggaaa aatatctcca attgctcgat caacgatgca
      961 agagattaaa tttttgatta actgatatga tgtctcttct ttcccaaaca tcaatttaat
     1021 tttgtttcct ataattgtat ttagactctc ttgatcaact ttcttctttt tattttttac
     1081 ttttaagact aatggaaaaa agtaaatgaa aatcaactta gacttaaatg acttatcaac
     1141 ttatgaccag gacctacttt taaacgagtt aaatagttca ttggaaggac taaacaattt
     1201 tcaagcgcaa aaacggttaa ctcaatatgg tacaaatgaa attctaagaa aaccctatca
     1261 tctaattatc attgaagcaa tctcgcactc aattaatcct ttagttgcca tcttattttt
     1321 tgccgcttta atttcagcat ttacgggaaa tgtagttaat gcagcgataa taattagcat
     1381 catcatcatt agcattggtc tggattattt tcaaagtcat cgcgctcttg ttgcaattaa
     1441 aatgttacaa aagaaaatag caactacagt aacagtctta cgtgaaaatc aatggctgga
     1501 gatacctgca aaagaattag ttcctggtga tttatttcga ttatccgctg gtgatatggt
     1561 gcctgcagac tcaatacttt taaaatcaaa agaccttcat attcaccaat ctgcacttac
     1621 cggggaatca atgccagttg aaaaggaagc aatcccacta aagaccaaac ctaaaaatcc
     1681 tcttgatgca ctcaatgtcg ttttttcagg ctcatctgta atcgggggca gcgcaacagc
     1741 actagcaatt aacacaggaa gtaatagttt atatgggcac atcgcagaaa gcttaaagaa
     1801 gaaaccacca catacagaat ttgaacaagg catgatgcga ttcagcactt ttatcatgaa
     1861 aatggtattt ctgttagtaa tctttgtatt ttcaatcaac atatacatgc acagaccatt
     1921 gcttgattcc ttgttgtttg ctgtagcgct agccgtaggt cttacacctg aattacttcc
     1981 aatgatcacg acagtaacct tagcctctgg tgccgttcgc atgtcaaaga aaaaggtaat
     2041 tatcaaaaat ttatcagcaa tccaaaattt cgggagcatt gatattttat gtagtgataa
     2101 aacaggcacc ctaacaagcg gagaaatgaa tttaacaaaa tacctggatt tttcaggaaa
     2161 gcaatccgaa tttgtaatgt tattagccta cttaaacagc gtatacataa cagaaattaa
     2221 gagtcccttt aatattgccg tattaaaaaa agcaagatta aatccattag atttagcaat
     2281 cttaaaacat gatcatcccg atattcaacc ataccataaa gtagatgaaa ttccttttga
     2341 ttttgagcgc agacgttcaa gtgtcattgt tagcaaaaat gagaatcacc tgttcatttg
     2401 caaaggcgca cctgaaaaca ttatgagcgt atgctcctat tatgattttg caggcgaaag
     2461 agaactgttt accgaaaaag aacaaaaaca atgcgaattg cttttccaat cgttaagcag
     2521 cgaaggttat cgagtcttag ctattgccta taaactaatg gatcgacaac tcaaatacac
     2581 tcaaagtgat gaaaaagaaa tgatctttgc ggggttttta gcctttactg atcccctact
     2641 tgaagatatc ccaaaagtaa ttaaagactt gcgtcacgaa ggaatcaata taaaaatctt
     2701 atctggtgat aatttaatag ttactcaaca tatctgtcaa aaagttggta tggatacaag
     2761 ccgtattttg acaggggaag aaatttcaaa aatttcggat gatgcacttc ccccactagc
     2821 agaacaaatt gatatttatg ctcgtataaa tcctatgcaa aaacagagga taatctctgc
     2881 tttaaaaaaa agagggcatg tcgtgggata tcttggagat gggataaatg atgtaccatc
     2941 tttacatcag gcagatgtgg gaatttcagt tgcttctgct gttgatattg ctcgtgaagc
     3001 agcagatatt gttttgctgg aacatcatct aaaagtttta ttaaacggaa tcatcgaagg
     3061 tcgtaagtcg tttagaaacg tcatgaaata tctcatgatg ggtacaagct caaattttgg
     3121 caatatgatt agtatggcaa ttgcagtcct gtttttacct tttttaccta tgctacccag
     3181 acaaatctta ttaaacaatt tactttatga catctcacaa ataacgatac ccactgatca
     3241 tgtagatcca agctcgatac atcgatataa gagattagat attaatataa ttcgccaatt
     3301 tatgttctac gttgggccta ttagctctgt atttgatttt ttaacatttt ttgtcatgtt
     3361 aaaaatattt tctgctaatg aagctttgtt ccaaacaggt tggtttgtcg aatctttggc
     3421 aacacaaact ttagttattt ttattattcg cacggccaaa aaaccttggc aaagtatgcc
     3481 aagtctccct ttaacaacta ctgttctact gatagttatg gtaggaatat ggttaccatt
     3541 tagcccattt tctggattat ttggatttgt gcccttgccg ccattgtatt ttcttttttt
     3601 ggttacagcg acttttactt acttatttct ggttgaatta attaaaaaaa agataatgcg
     3661 gaaattatat tagccaaaca acattatcat tcatttgaag gctcaaaatg attaaatgga
     3721 tttctgggtt cataattttg gttgtactta ttattgtcat tggtcatttt agtcaactgc
     3781 gagtttttct tggtttaatt gagcaaatga agttttcctg gctctttttt gcagttattt
     3841 tccaatcagg gacttatatg tcactagcag ccgtttggcg tttggcctta agatggtttg
     3901 gagaacaaat ttcttttttc cagctctttt tattaagttt ttctcaactt ttcgtaaatc
     3961 aaacgattcc gacaagcggt ttaagtggcg ttacaataat gaccaaattt ttattggggc
     4021 gtttgattac acccaaagct attgcattgg caattgcttt gaacgttttt tcgcgacaag
     4081 ttgcttatct gacatccttt attgcctcaa tagtcgtatt ttggttttac aatgagctca
     4141 acaaaacact tatttctcta tctctaatct ttatcctaat tatggtggct gtattaattt
     4201 tagcggccct gttatggcaa caggctttaa aattaaaaat tccaaagcta ttaaataaat
     4261 atacttctat tcgaatttta ataaacgaaa ttaagaagct cgatcccaag atagttttaa
     4321 acttcaaact ttttttcccc gctgtagtgt tctctgta
//
LOCUS       DASLOE010000208         4355 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.209, whole genome shotgun sequence.
ACCESSION   DASLOE010000208 DASLOE010000000
VERSION     DASLOE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.209"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..629
                     /locus_tag="U6309_002700"
     CDS             <1..629
                     /locus_tag="U6309_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEN5506114.1"
                     /translation="LADGNKIWFSLPKFLQKKLQSKGILYRRHYYGSGIQYKILRSIG
                     KNSGCMTWMERFQTDDQNKVEVMLQKMGVQFRWIQGNGLIIEQLLPACRNHPISGKLV
                     WFNQSNHANHYYNGTSDYIKSKINNTFSRFILLHKYFHPYIAFYGDGEPLSKQEADCI
                     NSAIQKNTVSTAWQPGDVMIVDNFSCLHGKTPHTGNRLILVGLTKYFD"
     gene            644..2536
                     /locus_tag="U6309_002701"
     CDS             644..2536
                     /locus_tag="U6309_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEN5506115.1"
                     /translation="MPDDISRKQFSIGDLYFTNELEVSGLIYEIFYQKSYLSDFLTLS
                     PGAVVFDVGANIGVFSLFVLKQCHYDTEIYSFEPVPATFKCLKKNLARFKHNVHVYNA
                     GIGNVPKDCSIDFTLFGESSVTATYKPTDKIISNYQPLLNYETLLKLSSFQNKSLYYQ
                     LKYLPFLRNYLIKKNYKHQTLETKVRCHLTALGRFIENNQITRIDLLKIDVEGAELDV
                     INSIKPEQFSLIKQLSIEVHDIDNRVEKLVSYLQKQGYITYVDRNPIFAELGFNHHMI
                     YAKLPEPAIITLSEEPNNPENYIEARQYFYGLLAGGVRIKLLESMFDLDLFRLFTGKP
                     YLLENDIIKRLELKPVRAKKWLHLLCCEDFLKKIMVGSQVGYQLAKSQLMLGEGYWGF
                     KQYYDFYWQRMANEKLSNILRFTDPKFNVSWPPKTAEEANFLETWMTKTATPLIQTLL
                     ACLDLNQYRSILDVGGGDGTIACALAQAYPHLKITVYNLPESAKIAQKNIDAMGLQKR
                     ISVFAGDFINDEQFPAGFDLILFVRVLWDWDNSRKRKLLNMAYHALKRKGHVAICEGF
                     KELCYDLCLTWEYSYIFADGFDAEVFKTSDEYKIMLQQIGFTPIPTKSISPSEPVPGT
                     VVLAEK"
     gene            3040..3654
                     /gene="lem15"
                     /locus_tag="U6309_002702"
     CDS             3040..3654
                     /gene="lem15"
                     /locus_tag="U6309_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem15"
                     /protein_id="HEN5506116.1"
                     /translation="MIYKITLTENNLAQAFMSIPQDVIALDLSNNLLGSKNCVDLLVN
                     IADCIPPGVTSLDLSSNGLRLTQVSLVTPKHVTSLNLCFNPLHEISIDGLEWLKDSLA
                     HIQIIYLSYDMVNNMSREQRQAFRAVIPNIQTIILVDKNGKEIGPSHTAKIANLIREL
                     GGGNPEVPSLLNQCTFFVKTQGINIDKAYIPEELKKRIHDCNPL"
BASE COUNT         1438 a          701 c          813 g         1403 t
ORIGIN      
        1 cattggctga tggaaacaag atatggtttt ctttaccaaa gtttttacaa aaaaaattgc
       61 aatctaaagg aatattatat cgaaggcatt attacggttc tggaatacag tacaaaatac
      121 ttcgatcaat aggcaaaaat tctgggtgca tgacatggat ggagcgattc caaacggatg
      181 accaaaataa agtggaagtg atgcttcaga aaatgggtgt gcaatttcga tggattcaag
      241 gcaatgggct aatcatagaa caattgcttc ctgcctgccg gaatcatccg atcagcggaa
      301 aactagtctg gtttaatcaa agtaaccatg ctaatcatta ttataatgga accagtgatt
      361 acatcaaaag taagattaat aacacttttt ctcgttttat cttgttacat aaatattttc
      421 atccttatat tgccttttat ggcgacggtg aacctttatc gaagcaagaa gcagattgca
      481 taaactctgc aatacaaaaa aatacggtat ccacagcctg gcaaccaggg gatgttatga
      541 tagttgataa tttttcctgc ttgcatggaa aaacacccca tacaggaaac cgattaatcc
      601 tggttggatt gacaaaatat tttgattaaa gggacccata ccaatgcctg atgatatttc
      661 acgaaaacaa tttagtattg gagatttgta ttttactaat gaattggaag tcagtggttt
      721 gatttatgag attttctatc agaaaagcta tttgtcggat tttcttactt taagtccagg
      781 ggctgttgtt tttgatgtcg gggcgaatat tggtgttttt tccctatttg ttttaaaaca
      841 atgtcattat gatactgaaa tttatagttt tgagcctgtc cctgctactt ttaagtgttt
      901 aaaaaagaat ttggcgcggt ttaagcataa cgtgcatgtt tacaatgccg ggataggaaa
      961 tgttccaaaa gattgtagta tcgattttac cctctttggg gaaagctccg ttaccgccac
     1021 ttacaaacca actgataaaa tcatttccaa ttatcaacca ttgttaaatt atgaaacatt
     1081 gttaaagctc tcttccttcc aaaataagtc tctttattat caattaaaat atttaccttt
     1141 tttgcgtaat tatctcatta aaaaaaacta taaacatcag actttagaaa ccaaagtgag
     1201 atgccacctg actgcattag ggcgttttat tgaaaataat cagatcactc gtatcgattt
     1261 actaaaaatt gatgtggaag gtgccgagct tgatgtaata aacagcataa agccagagca
     1321 gttttcgctt attaaacaac taagcattga agttcatgat attgataatc gggttgaaaa
     1381 gcttgtctcc tatctacaaa agcaaggtta tattacttat gttgatagaa acccaatttt
     1441 tgcagagctg ggttttaatc atcatatgat ctatgccaaa ttgcctgaac cggcaattat
     1501 tacactaagt gaagaaccaa ataatcctga gaattatatt gaagcacgac agtattttta
     1561 tggactcctt gccggtgggg tgaggattaa actgctggaa tctatgtttg atcttgattt
     1621 gtttcgtttg ttcaccggca aaccttattt attggaaaat gacattatca agagacttga
     1681 actgaaacct gttcgagcaa agaaatggct acatttgctt tgttgtgaag attttttaaa
     1741 aaaaatcatg gtcggttcgc aagtcgggta tcaattggct aaaagtcaat tgatgttagg
     1801 agaggggtat tggggattta aacaatatta tgatttttat tggcagagga tggctaacga
     1861 aaaattaagt aatatattgc gttttactga tccaaagttc aatgtttcct ggccaccgaa
     1921 aactgccgag gaagctaatt ttcttgaaac atggatgaca aaaacagcaa caccacttat
     1981 acaaacttta ttggcttgtc ttgatttaaa tcaatatcga tccatattgg atgttggggg
     2041 cggggatggc actattgcct gtgcactggc tcaggcctac cctcatttaa aaattaccgt
     2101 ttacaattta cctgaatcag caaaaatagc tcaaaaaaat atagatgcaa tgggattaca
     2161 gaagcgaatt agtgtatttg cgggtgattt tattaacgat gaacaatttc ctgcgggatt
     2221 tgatttgatt ttattcgtgc gtgttttgtg ggattgggat aattccagaa aaaggaaatt
     2281 attaaatatg gcgtatcatg ctttaaaaag gaaaggccat gttgctatct gtgaaggctt
     2341 taaagaactt tgctatgatc tttgtctgac atgggaatac agctatattt ttgcagatgg
     2401 atttgatgcg gaagttttta aaacgtcaga tgagtacaaa atcatgctgc agcaaatagg
     2461 ttttactcct attccaacca aatccatttc tccttcggag ccagtgcctg ggacggttgt
     2521 attggcagaa aaatagtttt catcagccat accgattggc atagacccat ttaacacaat
     2581 taagggctca gggttttgta attttgaaat tataaaacgc ggttaaagtg tggtttactc
     2641 ctatttttgt tttataggaa tagtatgttt gtttccttat ttcaaaaact aaaggcctta
     2701 tcccaaaatt tcaacgttag tttttatgct ttttatgttg tctgatggtc gatttcaatt
     2761 cttttcaaat tgattaatta cacactaaaa attgcttaga taaacaggcc aatggataca
     2821 atcccataga tagaggatta agtattccaa tacagaatgt tcattaaatt tttttaacaa
     2881 atgtaggtag atctttgatc cgacatttct tactttgata cggagcacat aagagatttg
     2941 tatatttaat atacaaacaa tctattttaa ttataatcaa aattatcata ttatgaccaa
     3001 taagaataat atttattcat taatattgag gttttaatta tgatttataa gataacatta
     3061 actgaaaata acttggcgca agcttttatg tctatccctc aagatgtaat cgctcttgat
     3121 ttgagtaata atctcctggg ctcaaaaaat tgtgtagatt tattagtaaa tatagcggac
     3181 tgtatacctc cgggggtgac ttcgcttgat ttgagtagca atgggctcag attaacgcaa
     3241 gtctctttag ttacacccaa acacgtgact tcacttaatt tatgttttaa tcccttacat
     3301 gaaatatcta ttgacggttt agagtggctt aaggattcgt tagcacatat tcaaattatt
     3361 tatttaagct atgatatggt caataacatg tcaagagagc aacgacaggc atttcgagcg
     3421 gtaattccca acatacaaac aattattttg gtcgataaaa atggcaaaga aattggtcct
     3481 tcacatacag caaaaatcgc taatctcatt agagagcttg gaggtggaaa cccagaggtt
     3541 ccctctttgt taaaccaatg tacctttttt gttaaaactc aaggaataaa tatagataag
     3601 gcctatattc ccgaagaatt aaaaaaacgt attcacgatt gcaatcctct ctgatttatt
     3661 gttgcgtagt agcaagtgta ggtcgggcct tggcccgaca acttttgcta attatctaag
     3721 atattcgagc aggaaagggg aaaaaatgaa aaaaagtccc aggattaaat aaagtaaaaa
     3781 ctgatcctgg atgagcattt ggtctttttt tcttggacga agtatataaa atgcaacaaa
     3841 aagacaggca acaccaatca atatgcttaa aatttgtact aacaaaaata tagacataaa
     3901 ttgtggagaa gtggtctggg caatgtgctc ttctaccggg gtgactttgg cagcatatga
     3961 attcattttt tagcttaagt aaagagattg ttataatcct cgctccattt aaccaagatt
     4021 tcaagtgcat gggaacaatc tttctctacg attagaaaaa tgctggcttt tatgatcatc
     4081 gctttggcag gactcataac atagggaagg ggtagacaaa taaacgccaa attaagtgga
     4141 taactgtaaa aaccaaaaag atccatgagc cagcttcttc aaatactacc ttgatttatt
     4201 cacgataaac tcagcaactt tgcaataaaa gccgctgagt ttaggaaaat tatcggaaaa
     4261 tttgcctatt gaaaaaaata aacaacagtt ggcgcatgaa attttgctct acaccgaggt
     4321 aaattcattt ttttgataga taggttaata aaatc
//
LOCUS       DASLOE010000209         4323 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.210, whole genome shotgun sequence.
ACCESSION   DASLOE010000209 DASLOE010000000
VERSION     DASLOE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.210"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..2691
                     /locus_tag="U6309_002703"
     CDS             <1..2691
                     /locus_tag="U6309_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5506117.1"
                     /translation="CSKQSRDIRDNKKFEPVSEITDLKNLLKHKDVEKIINDITQFDE
                     DQRELKRIERLIQKDEEKEDNKKMHLGKRSKLELRRAELSKRVQLFSNKLKNVPQAEE
                     IKAVLDYCQKYIEKHINLSKQLPKGMLDVKRALSLFDEINSTKTQIRKLSKDKLDSSG
                     QIKLEGLKKELTKAELALQFSYSEEEDKTIKDMLNDLKRLMKMSIRDYQKLATSGGEI
                     NVFLHKTTGFYWESLFLYRNVLCEINEYLKKNKKKLSEQGMLVAECISKAFSQMPELD
                     TIEKALEKVKRIDDDLKRQEEYNEKINGTWIRFYKKGPVKFVQQVLEQNLNEKIFYIP
                     YNPHNYVLDHPAQSDFADTLGNCYGETQMFLKQINSKSPAINNICPERVLINYQLDQS
                     RTIAKNTKKEKIGIFEADEKTKAQVTWDGIKDILTSKVTSTAFGDVCMITLMGAQVSG
                     REKLVGHGIGLIKMKNPAPYKYVVYDYAFGGAMGFSSDTQLELFFKQILEGEGSYVPF
                     KKCLVEKVGEVSKECQQLINGNPGIASLTQKSLDKTCNRDFWDKARIGLLIKYHPIGT
                     DENRILDLIEKLPLSKDRLELYGQLIKNPKIGIGNLVKCIICSGKISYLANLLTSDLL
                     TVDITNKIKFKLNEQAKKEIVALINNDQIEPNLTCKLFSNNQTIVLAAYKKDKNSLQY
                     ADIDQVVTLLQCKNTITPSDACRAFPKAEKIVIAAYKSDPSSLQFANLGLVQQLLRKG
                     SITPEHASKAFTENSQFHKAHEAYQFLKSLKPILKHENETEKAKRAELLKWIDQKLEI
                     TNLRQELYPLISNMCILLLDDIRILSPEDTIIKNSRLSKLISSNQCDINILKTLENKK
                     MELSKQCSLTKSNFTFFGAADNKVDGGNANTPGHITPENK"
     gene            3049..>4323
                     /locus_tag="U6309_002704"
     CDS             3049..>4323
                     /locus_tag="U6309_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5506118.1"
                     /translation="MQEKIINLGKGLKKVNEGIKKHISRAEEFLYLANQWEKENVKIN
                     DLLDGVQFSVNDKKMSGQEIKEELSNLIERYNNFLKKNLKGERSETLELKEIQQQLIN
                     FYNAVNDIYTYLSINKTEFFLEAIKSCYKKDQRMSSTIQFVLKIADHLSKERLQFNLN
                     LNSQFAEASESFQEVTTKFYREVATPRMESANKLLELMNNFVDQYISQGASPEDVIKK
                     LKPFEKYFVIEISHYEEFGHLVYKTNFNCNPALFDVNPIEFLNELFVQFYKDTTLSSR
                     SKAALKKVLDKFEITYEVPKTWYSYIDWDIPIVTDKNSVLVTYDKQAYNKYHQESGQR
                     EMEFFETIERCLDKSKDIIIATKKESLKDQKEFCKLIDKAIKELEETKKKKIFQKEPH
                     ELVTSIINKSIKLLHDLKNNMPLRREDLVIVIL"
BASE COUNT         1662 a          622 c          770 g         1269 t
ORIGIN      
        1 tgttccaagc aaagcagaga cattagagac aataaaaaat ttgaacctgt ttctgagatt
       61 accgatttga aaaatttatt aaaacacaag gatgtcgaaa aaattatcaa cgacattact
      121 cagtttgatg aagatcaaag agaacttaag agaattgaac gtttgataca aaaagatgaa
      181 gagaaagagg ataataaaaa aatgcatctt ggtaaaagat ccaaattaga gcttaggagg
      241 gcagagcttt ctaaaagagt tcaattgttt agtaataagt taaaaaatgt tccgcaagca
      301 gaggaaatca aagccgtttt agattattgt caaaaataca tcgagaaaca tatcaacctt
      361 agcaagcaat taccaaaagg aatgcttgat gttaaacgag cattgtcttt atttgacgag
      421 ataaattcca ccaagaccca aatcaggaag ttaagcaaag acaaactgga ttcttcaggg
      481 caaataaaac ttgaaggttt aaaaaaggaa ctcacaaagg ctgaattggc tttgcaattt
      541 agttattcag aggaagaaga taaaacgatc aaagacatgc tcaatgattt gaaaaggctt
      601 atgaaaatga gcatcagaga ttatcaaaag ttagctacat ctggcggaga aataaatgtt
      661 tttttgcata agacaactgg tttttattgg gaaagccttt ttctgtacag aaatgttttg
      721 tgcgaaatta atgaatatct taaaaaaaat aaaaagaaat tgtctgaaca aggaatgctg
      781 gtagctgaat gcatttctaa agccttcagc cagatgcctg aattggatac cattgaaaaa
      841 gctttagaaa aagtcaaaag aatagatgat gatcttaaaa gacaagaaga atataacgaa
      901 aaaatcaatg gaacttggat taggttttat aaaaaaggtc cagtaaaatt tgttcaacaa
      961 gtccttgaac agaacttaaa tgagaaaatt ttttacatac cgtataatcc gcataattat
     1021 gtgctggatc atcctgccca atctgatttc gccgatacct taggaaattg ctatggcgaa
     1081 actcagatgt ttctcaaaca aataaacagt aaatctcctg caattaataa tatttgtcct
     1141 gagagggtat taatcaatta tcaactagat caatccagga caattgctaa aaatacaaaa
     1201 aaagaaaaaa tagggatatt tgaggcagat gagaagacaa aagcacaagt aacatgggat
     1261 ggaattaaag atatcttaac atcaaaagtt acctctaccg cttttggtga tgtgtgcatg
     1321 atcactttga tgggagctca agtttctggt cgtgaaaaat tagtaggtca tggtattggc
     1381 cttatcaaaa tgaagaatcc tgctccttat aaatatgtgg tatatgatta tgcttttggt
     1441 ggagcaatgg gattcagtag tgatactcaa ttggagctat tctttaaaca aatccttgaa
     1501 ggagagggat cctatgttcc ttttaagaaa tgtctggttg aaaaagtagg agaggtgagt
     1561 aaagaatgtc aacaacttat taatggtaat cctggtattg cttctctgac acaaaaaagt
     1621 cttgataaaa catgtaatag agatttctgg gataaagccc gcattgggct tttgataaaa
     1681 tatcacccaa ttggtacaga tgaaaacagg atacttgatt taatagaaaa gttacctcta
     1741 tctaaggata gattagagct atatggacag cttattaaga accctaaaat tggtatcggt
     1801 aatcttgtta agtgcatcat ttgctcaggt aagataagtt acctggcaaa tcttttaaca
     1861 tccgatttat taaccgttga tattacaaat aaaataaaat ttaagttgaa tgaacaagct
     1921 aaaaaagaaa tagtggcttt aattaataat gatcagattg aaccaaactt aacttgtaaa
     1981 ctattctcta acaatcaaac gattgttttg gcagcctata aaaaagacaa aaactcgctc
     2041 caatatgcgg acatagacca ggttgtaacg cttttgcaat gtaagaatac cataacacct
     2101 tcggatgcct gtcgggcttt tcctaaggca gaaaaaatag tcatcgcagc ctataagtcc
     2161 gatccatcat cgttgcaatt tgctaatctc ggtttggttc aacaattatt acgaaagggt
     2221 tctattactc ccgagcatgc ttctaaagct tttacagaga actcacaatt ccataaagcc
     2281 catgaggcat atcagttcct caaatctctg aagcctatct taaaacatga aaatgaaact
     2341 gagaaagcca agagagccga gttattaaaa tggattgatc aaaagctgga aattactaat
     2401 ttgagacaag aactttatcc cttaatttca aatatgtgta ttctattact tgatgatatt
     2461 cgaatccttt cccctgaaga tacaataatc aaaaacagca gattgtccaa gttaatcagt
     2521 tcgaatcagt gtgatatcaa tatattaaaa acacttgaaa ataaaaaaat ggaattaagt
     2581 aaacaatgtt ctctgacaaa atcaaatttt actttttttg gagctgcaga taataaagtt
     2641 gatggaggaa atgctaacac tcctggccat atcactccag agaataaata aaagcgtttt
     2701 gttggaattg gtcgtataga tgcggccaat ttgttaaatt gaattagttt cggcttaatc
     2761 tgacacaact gcttgaacac ttgagcgctt ccggttttgg ggcattttat gttcgggaat
     2821 ttattcaaag agttttttac ctctagctaa ctttaattac cgaaacaacc tatcaaatta
     2881 aacatatcaa taaaatcttc tattaatccc gaacactatc tttcccaagc aatgatttat
     2941 ataaatcaat tgaatttatt aaggtcaata tgattgaaaa ttaatttaat ggtctattta
     3001 atagactata attaaaaaca taagtcacat cgaatctgga atcatgtaat gcaagaaaaa
     3061 attatcaact tagggaaggg gcttaaaaag gtcaatgaag gtattaaaaa acatatttct
     3121 cgagcagaag agttcctcta cttggcgaac caatgggaaa aagagaatgt taagattaat
     3181 gatttattgg atggagtgca gttttcagtt aatgataaaa aaatgtcggg tcaagaaatt
     3241 aaagaagaac tatctaatct aatagaaaga tataataatt tcctaaaaaa aaacttaaaa
     3301 ggggaaagaa gtgaaacatt agaactaaag gagattcagc aacaacttat caatttttat
     3361 aatgctgtta atgatattta tacttattta tccataaata aaacagagtt ttttcttgaa
     3421 gcaatcaagt cttgctacaa aaaagatcag aggatgagta gtacaattca gtttgtgctg
     3481 aagatagcgg atcatctttc taaggagaga ctacaattta atttgaattt aaattcgcaa
     3541 tttgcagaag cttcagagag ttttcaagag gtaactacca aattttacag agaagttgct
     3601 actcctagaa tggagtctgc aaacaaattg ttagagctga tgaataattt cgtagaccaa
     3661 tatatatcac aaggggcaag tccagaagat gtaataaaaa aacttaagcc atttgagaaa
     3721 tattttgtta ttgaaatttc gcattatgaa gagtttgggc atttagtata taaaacaaac
     3781 tttaattgta atccagcgtt atttgatgtt aatcctatcg aatttttaaa tgaattattc
     3841 gtgcaatttt ataaagacac cactttatca tctcgctcta aagctgcttt aaaaaaagtg
     3901 cttgataaat ttgagattac ttatgaggtg ccaaagacct ggtatagtta tattgattgg
     3961 gatattccta ttgtaactga taaaaattca gtattagtta cttatgacaa acaagcttat
     4021 aacaaatatc atcaagaaag cggccagaga gaaatggagt ttttcgagac aattgaacga
     4081 tgtttggata agtcgaaaga cattatcatt gcgactaaaa aggaaagttt aaaagaccaa
     4141 aaagaatttt gtaaattgat agataaagct attaaggaat tagaagaaac aaaaaagaaa
     4201 aaaatttttc aaaaggagcc gcatgagctg gtaacaagta ttatcaataa atcaataaag
     4261 ttgttacacg acttgaaaaa taatatgcca ttaagacgag aagatttggt catagtaatc
     4321 tta
//
LOCUS       DASLOE010000210         4293 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.211, whole genome shotgun sequence.
ACCESSION   DASLOE010000210 DASLOE010000000
VERSION     DASLOE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.211"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..893
                     /locus_tag="U6309_002705"
     CDS             <1..893
                     /locus_tag="U6309_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HEN5506119.1"
                     /translation="AGIILAKAMPNGLRSRERIAFFLRANNQLYTTLNKSRTIQFHFF
                     DLFADFDTHIQRLNAIQPTIISAPASVLLMLASHQDILKITPKKIFSVAEVLEPKEEE
                     ILTKAFGCAISQIYQCTEGFLAISERKTNRLLMNEEFLIIEKEWLDEERFVPIITDLY
                     RSTQPIIRYRLDDVLVAERSNSVFTELKNIEGRLGDVCYGKRGQEIVPIFADVIRQHM
                     ISSGVSFEDYLICQQTLTQFTIQISPEICNKNQFIEHLNQLFIYKNCELPQWNWELYD
                     KNKPGVKRRRVRSLYCPKTE"
     gene            complement(987..1847)
                     /locus_tag="U6309_002706"
     CDS             complement(987..1847)
                     /locus_tag="U6309_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506120.1"
                     /translation="MKKILITLIGFLFMNTYYHLLYADNSHHHSVKDLSTSSSKLENT
                     PTIQLEVQKIIDKKDKKLVFVKLTDIKSNKPITFNNLTEIHTKKFHLLIIDNHLIDYS
                     HVHPAETATPGIYQFEWQPNFKNATYRAWADLLPTNTNKQEYATADLTSPQTVTRTMN
                     PIHHRTVLESTVNGYQFKLTFDKMPLLAGKPAMGEIDVTDSKGHPVRTLEPIMGAYAH
                     IVGFNEDFKTVIHVHPMGKEPVNNTERGGPQLQFHIEPNKAGFIKMFAQVQIDGKVLY
                     VPFAFTVKNA"
     gene            2056..4263
                     /locus_tag="U6309_002707"
     CDS             2056..4263
                     /locus_tag="U6309_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN5506121.1"
                     /translation="MAHNHHEGHSHVHNEHLCCHKKDNAYKPQIISDGTIFYTCPMHP
                     EVREATPGICPLCGMALEPEAISIGEATNPEYLDMKHRFWVALILTLPVVILEMAGHG
                     FQHFVSTSISNWIQLLFATPVVLWCGWPFFLRGWQSLKTRQLNMFTLIAMGIGVAWIY
                     SVVAVFSPGLFPAAFRSQEGVVSVYFEAAALIATLVLLGQVLELKAREQTGSAIRALL
                     KLAPENAHRIKEDGSEEEIALNQVEVGDSLRVRPGEKIPVDGEIQEGHSFIDESMVTG
                     EPMPVAKEIGDKVIGATINQTGSFIMKALHVGADTMLARIVQMVSDAQRSRAPIQRVA
                     DTVSAWFVPAVILVAVLSFIIWGLLGPQPSLGYGLIAAVSVLIIACPCALGLATPMSI
                     MVGVGKGAQNGVLIKNAEALERMEKVNTLIVDKTGTLTEGHPKLTHIETSEDWDERQA
                     LLLAASLEFHSEHPLASAIVKAAQEMQIAFSSVQNFEAPTGMGVVGRVNSHHVAIGNA
                     KLMHEYGSDDVSLFEKADKFGAKGSSVMFMAVDGKTVAFLVVEDPIKSTTAETIHALQ
                     KKGIEIYMLTGDSKKTAEAVAATLGIKKVIAEIMPEDKGRIVSELKENGLVVAMAGDG
                     VNDAPALAKADVGIAMGTGTDVAIESAGVTLLHGDLRGIAKARYLSEYTMNNIRQNLF
                     FAFIYNMLGVPIAAGIFYPLTGLLLNPVIAAAAMALSSVSVIVNALRLRQVTL"
BASE COUNT         1266 a          759 c          955 g         1313 t
ORIGIN      
        1 gggccggaat aatccttgcc aaggctatgc cgaacggttt gcgttcgcga gaacgaattg
       61 ctttttttct aagagcaaat aaccagttgt ataccacttt aaataaaagc agaacaatac
      121 aattccattt ttttgatctt tttgctgatt ttgatactca tatccagaga ttaaatgcca
      181 ttcaaccgac tattatttca gctccagcca gtgtgctttt aatgcttgct agtcatcaag
      241 atattttaaa aattacacca aaaaaaatat tttctgtggc cgaagtactg gaaccaaagg
      301 aagaagagat tttaactaaa gcatttggtt gtgccatatc gcaaatttat caatgtacag
      361 aaggttttct ggctattagt gaacgtaaaa ccaatcgttt attgatgaat gaagagtttc
      421 ttattattga aaaggaatgg ttggatgaag agcgattcgt gccaattatt actgacctgt
      481 acagatctac tcagccaatt attcgttacc gtcttgatga tgttttggtg gctgaaagat
      541 cgaatagtgt atttactgag ttaaagaata ttgaaggccg tttaggtgat gtttgttatg
      601 ggaaaagagg gcaagaaata gttcccattt ttgcagatgt tattcgacag catatgatta
      661 gcagtggtgt aagttttgaa gattatttaa tttgccagca gacattaacc caatttacaa
      721 ttcagatatc acccgaaata tgcaataaaa atcaattcat tgaacatttg aatcaacttt
      781 ttatctataa aaattgcgaa ttaccccaat ggaattggga gctctatgat aaaaataaac
      841 ctggtgtgaa acgcagaagg gtccgctcac tatactgccc taaaacagaa tgagcgggta
      901 caaagaactg gtttgctctg cacaacacga gccaggagct tacaataata tttacgtctg
      961 taaaagacag gttcttaatt tagtcgttaa gcatttttta ctgtgaatgc aaatggtacg
     1021 tagagtactt tcccatcaat ttgcacctgg gcaaacattt taatgaaacc tgctttatta
     1081 ggctcaatat ggaactgcaa ttgaggtcct ccacgctcag tgttatttac aggctctttg
     1141 cccatggggt ggacatggat aacggtttta aaatcctcat taaagccgac aatgtgtgca
     1201 taggccccca taattggttc tagtgtacgg actggatgcc ctttagaatc agtcacatcg
     1261 atttcaccca tcgctggctt acctgctagt aaaggcattt tatcgaaggt taatttgaat
     1321 tgatatccat tcactgtact ctctaatact gttcgatgat gtataggatt cattgttctc
     1381 gttactgtct ggggagatgt taaatcagca gtggcatact cctgtttatt ggtattggtt
     1441 ggtaacagat cagcccatgc ccgataggtg gcattcttga aattgggttg ccattcaaat
     1501 tgataaatcc ccggagtagc agtttctgcg ggatgaacat gactatagtc aatgagatga
     1561 ttatcaatga tcagtaaatg aaattttttt gtatgtatct cagtaagatt attaaaagta
     1621 attggtttat tcgattttat atcagtgagc ttgacaaata ccagtttctt atccttttta
     1681 tcaattatct tctggacttc caattgtatt gtgggtgtat tctccaattt ggaggaagat
     1741 gttgaaaggt ctttcaccga gtgatgatgg gaattatcgg catagagcaa gtgataatag
     1801 gtgttcatga ataaaaagcc aattagcgtg atgagaattt ttttcacaat acaatgatct
     1861 ccgtaatttt ttattgccaa gctaaatttt tcttctgtca acgatgaatt ataattgaaa
     1921 gtagctgatt cttacagggt aaaaaatgca ttatttatta tgaccttgta gatataaacc
     1981 agtacaacag ggtatcatga gctaatttaa atggttataa attccaggca gaataaaact
     2041 acggataggt gaataatggc acacaaccac catgaaggtc attcccatgt tcataatgaa
     2101 cacctttgtt gccacaagaa agataatgca tataaaccgc aaattatttc agatggaaca
     2161 attttctata cttgccccat gcatcccgaa gttcgcgagg ctacccccgg gatttgtcca
     2221 ctttgtggaa tggcattaga accagaagca atttccattg gtgaagcaac aaatccggaa
     2281 taccttgata tgaagcaccg attttgggtg gcgttgatat tgactttgcc tgtagtaatc
     2341 ttggaaatgg ctgggcatgg attccagcat tttgtttcga caagtatttc aaattggatt
     2401 caattattgt tcgcaacacc tgtggtgcta tggtgcggtt ggcctttttt cctgcgtggt
     2461 tggcagtcac tcaaaacacg ccaattaaat atgtttactt tgatagcgat gggtattggt
     2521 gtcgcatgga tttatagtgt ggtggcggtt ttttccccgg gattattccc tgctgcgttt
     2581 cgaagtcagg aaggggtggt ctctgtgtat tttgaagcag ccgctttgat agctacactt
     2641 gtactgctgg ggcaagtact tgaattaaag gcacgggagc aaacaggtag tgccattcgt
     2701 gctttactga aattagcacc agaaaatgcg catcgcatca aggaagacgg tagcgaagag
     2761 gaaatagcac tcaatcaagt agaagttggt gattcgctgc gtgtacggcc aggtgaaaaa
     2821 atacctgtgg acggtgaaat acaagaagga catagtttta tagatgaatc tatggtaacg
     2881 ggtgaaccta tgcctgttgc taaagaaatt ggagacaagg ttattggagc tacaattaac
     2941 caaacaggta gcttcattat gaaagcattg catgttggtg cagataccat gctggcacgt
     3001 attgttcaaa tggtgagtga cgctcaacga agccgtgctc caattcagcg tgtcgcggat
     3061 actgtctcgg cctggtttgt tcccgcagtg attttggtag cggtactgtc ttttattata
     3121 tgggggctat taggtcccca gccatctctt ggttacggct taattgctgc tgtatcagtt
     3181 ctcattattg cttgcccatg tgcattgggt ttggcaacac caatgtctat catggtaggt
     3241 gtggggaaag gcgcgcaaaa tggagtatta atcaaaaacg ctgaagcgct tgagcgaatg
     3301 gaaaaggtaa atacgctcat tgtagataaa acgggcacac ttacggaagg acacccgaaa
     3361 cttacccata ttgaaaccag cgaagattgg gatgagagac aagcattgct attagctgct
     3421 tctctggaat ttcacagtga acatcccctg gctagcgcta ttgtgaaagc agcacaagaa
     3481 atgcaaatag ctttcagctc agttcaaaat tttgaagcgc caaccgggat gggggtagta
     3541 ggtagagtaa atagtcatca tgtagctatc ggtaatgcaa aattaatgca tgagtatgga
     3601 agcgacgatg tttctctttt tgaaaaagct gataaatttg gggcaaaagg ttcttctgtt
     3661 atgtttatgg cagttgacgg taaaacagta gcgtttttag ttgtagaaga tccaattaaa
     3721 tctactaccg cagaaaccat tcatgccctt cagaaaaagg gtattgagat ttacatgctg
     3781 actggggata gcaaaaaaac cgcagaagct gttgcagcta ctctgggtat aaaaaaagtt
     3841 atagcagaaa taatgcctga agataagggt cgtattgtca gcgaattaaa agaaaatggt
     3901 ttggttgtcg ccatggcagg cgatggagta aatgatgctc ctgcattggc aaaagcagat
     3961 gttggtattg ctatgggtac aggcactgat gttgctattg agagcgctgg cgttaccttg
     4021 ctacatggag atttacgtgg tattgccaaa gctcgatatt tatcagaata cacaatgaat
     4081 aacatacgcc aaaatctctt cttcgctttt atttataata tgttgggtgt ccccattgcc
     4141 gcaggtattt tctatccatt aacaggtctg ctcttaaatc cagtcattgc tgcagcagct
     4201 atggcacttt cttctgtatc agttatcgtt aatgctctga ggctgcgaca agttacactg
     4261 tgaatcggta gtaaagtgta ttaaatcatt ctg
//
LOCUS       DASLOE010000211         4224 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.212, whole genome shotgun sequence.
ACCESSION   DASLOE010000211 DASLOE010000000
VERSION     DASLOE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.212"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            194..1744
                     /locus_tag="U6309_002708"
     CDS             194..1744
                     /locus_tag="U6309_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEN5506122.1"
                     /translation="MSPRICCYNQYPGLYQDFDAMREDLQRIQKMGFKQVWVNPFYTP
                     CQYNPVPNMANRIHSPYAMQDESIYPRYAKNEKSVQQYTAKANELGLIPVFDLVARHL
                     AVDHRFVNGDKFFLEKYNIDTKKWFKRHLNGNLVMHNMDENYNPLTNNPWSDVATFNY
                     DDPVICEQIIEYYWKPFIERNIEHLGFQGIRIDAPAMVNNKVLSRLTEIAKEACQRKF
                     QRDPLIIAETIGRGYMEENLALKGIVTHTMNSVFWMPGPEGGYGYSFDLWQQDDNWFT
                     QNKGLLQQVGPTIGFPGSHDEPRYIQQLVEKGIHDDKLLAKCMREKLAVSAFCSDGGW
                     ILQYGDEYGATKPVNVFDPTPVEYHQQNHLRRFDLSDYISEINTTLAQLPNPHFPEWA
                     QRVFLPNHPDLVTFIVHQGWGYTGTSFVIATNTDPSQQIQLTEKELGEIMLANGRNNT
                     TEKQIKPQVLYLCGAVRASPELKKEIQVVASRPTASEKSPQFFQSHPEEKKTGEKLHS
                     DNTPPQFK"
     gene            complement(2017..3747)
                     /gene="lem27"
                     /locus_tag="U6309_002709"
     CDS             complement(2017..3747)
                     /gene="lem27"
                     /locus_tag="U6309_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem27"
                     /protein_id="HEN5506123.1"
                     /translation="MVRVLNLQKGVNMFNVDNSGKGNCLYYAYSISLMYYLRAKNNVR
                     ITEDIFNKLGLKEEDRISLRKLLSKDPDRAFTREEIKTIIEPILGRATRDLAAEHTKV
                     EFKSSPHDTPLFSSLHYAVEFGFKRSLQINESELTLLIDNDFSNPDYTEAEIYKVSGL
                     LDALQEYILTRTPSVIEEFNRQWENKKLELKEQSLTEKENQVHQATILDNILRKETID
                     FLLADNEKHLDEYREHLRREFVWGSEETLMVLHRAIQGERMVRNHEGRIEPVYDHEII
                     LHVHRNGASPSYQAGSPEMILNNEGNVHWTSIIPDAIFTSKLTDKEEKLLEMLERMQS
                     EHGSKELGVEKYSSISDWLRDLMKQIELIKTSPTAVTKQKEIELFFQLLAKATPKLAS
                     EPALRMSLGTLFSNFLECIPALMVENKLSTGLAKFGLYSPERSPVISQKKEDLSLTKS
                     SEEKVNSDQSQKGVLSQFSKHTLLKVSEEITPKPYPTELNPYVRKNKQSGPRVARALA
                     QMYQKGLEGDERYTPEKCKDIAQRYIEYSKSHKRNFILDDVILFMQEMDKIIKQKEAI
                     SEVTCKLVAM"
     gene            complement(3741..3992)
                     /locus_tag="U6309_002710"
     CDS             complement(3741..3992)
                     /locus_tag="U6309_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506124.1"
                     /translation="MSICWDLIINHNTPLQTNFTGHRVQMNDSESTPKGILFVSNKLF
                     INLLTYITEYQSITRKELMNLSNNEIKPRRKSNKRLLMW"
BASE COUNT         1275 a          810 c          784 g         1355 t
ORIGIN      
        1 taaaatatat actataaaat ataacaaagt gcttaataaa agtaaacatg atatccataa
       61 agagcaaaca acacactttg gttaactttc taagcatgca taggaattaa atggttggta
      121 aaaatacaat agggtcgaat ttaatacttc tactatactt catatacata gaaacagaaa
      181 ttgagaagtc agcatgtctc cacgtatttg ttgctataac caatatcctg gcttatatca
      241 agattttgat gcgatgcggg aagatttgca gcgaattcag aagatgggtt ttaagcaagt
      301 ttgggtcaac ccattttata caccttgtca atataatcct gtaccaaaca tggctaatcg
      361 tattcatagc ccatatgcca tgcaggatga atcaatttat ccacgctatg ccaagaatga
      421 aaaaagcgtt cagcaataca ctgcaaaagc aaatgagctt ggtttaatac ctgtatttga
      481 tttggtcgca agacacctag cggtagatca tcgttttgtg aatggcgata aattttttct
      541 tgaaaaatac aatatcgata cgaaaaaatg gtttaaacgt cacctcaatg gaaatttggt
      601 gatgcataac atggacgaaa attataatcc gttgaccaat aatccctgga gtgacgtggc
      661 aacgtttaac tatgatgatc ctgtcatttg cgaacaaatt atcgaatatt attggaaacc
      721 atttatcgag cgtaatattg agcatcttgg ttttcaggga atacgtattg atgccccagc
      781 catggtaaat aacaaggtat tgtctcgact aacagaaatc gcaaaggagg cttgccaacg
      841 caaatttcaa cgtgacccac ttattattgc agaaacgata ggcagggggt atatggaaga
      901 aaatcttgct ttgaaaggta ttgtaaccca cacgatgaat agtgtgttct ggatgcctgg
      961 ccccgaaggt ggctatggtt attcatttga cttgtggcaa caggatgaca attggtttac
     1021 tcaaaataaa ggattattac agcaggttgg tcctacaatt ggttttcctg gctctcatga
     1081 tgagccacgc tatattcagc aactggtgga aaaaggtatc catgatgata aattgcttgc
     1141 aaaatgcatg cgcgaaaaac tggctgtcag cgcattttgt tctgatggcg gttggatttt
     1201 acaatacggt gatgaatatg gtgctaccaa gccagtaaac gtttttgatc caacccctgt
     1261 tgaatatcat caacaaaacc atctgagacg atttgatctg tcagactata tctctgaaat
     1321 taatacaaca ctggcccagc tacccaatcc tcacttcccc gaatgggcgc agcgggtctt
     1381 tcttccgaat catccagatt tggtaacctt tattgttcac cagggttggg gttatactgg
     1441 gacctcattc gttattgcga caaacaccga tccctctcag caaatccaat taacagaaaa
     1501 agaacttgga gaaataatgc tggcaaatgg ccgaaataat acgactgaaa aacagataaa
     1561 accacaagtg ctttatttat gtggcgccgt tcgcgcatca cctgaattga aaaaagagat
     1621 ccaggttgtt gcatcaaggc caactgcaag tgaaaaatcc ccccaatttt tccagagcca
     1681 tcctgaagaa aaaaaaacgg gcgaaaagtt acattcggac aatacaccac ctcaatttaa
     1741 ataacaggta ccaggcttag ctctaaaacc acgtttactg gtgagttgat tgttatcata
     1801 ttgcttactc accaatcagg acctcacttt gataaggaag cagtatggct ttgatgattt
     1861 tatcgttcct gctttaaaaa aagcaggaac ctctctcttg cataaactga ttattttgtt
     1921 ttagagcaag gtgcaaacgt tttgaggagc agtgtttaca tccagtaaat aagcacctca
     1981 gaaacgtttg aaaagcgaca ggttgtgtca gttttcctac attgctacaa gtttacaggt
     2041 aacttcgcta attgcttctt tttgcttaat gattttatcc atttcttgca taaagaggat
     2101 gacatcatca agaatgaaat tgcgtttatg agatttcgaa tactctatat acctttgagc
     2161 aatgtctttg cacttctccg gggtatatct ttcgtcgccc tcaagtcctt tttggtacat
     2221 ctgtgcgagt gctctcgcta cgcgtggacc agactgcttg ttttttcgaa cataagggtt
     2281 tagttctgtg gggtatggtt ttggggttat ctcttctgat accttaagca gggtatgttt
     2341 cgaaaattga cttaataccc ccttttgaga ttggtcagaa ttgactttct cctcagatga
     2401 tttagtcaag gacaagtcct cttttttctg agatattaca ggactgcgtt ccggactgta
     2461 caatccaaat ttagctaagc ctgtgctgag tttattttca accataaggg ctggaatgca
     2521 ttcgagaaaa ttggaaaaaa gagtaccaag cgacatcctc aaggctggtt ctgatgctaa
     2581 tttaggtgtt gctttagcca gaagctgaaa aaataattca atttcttttt gttttgtcac
     2641 agcagttggg ctggttttaa taagctctat ctgcttcatc aaatctctaa gccaatcact
     2701 tatcgaagag tatttttcta ccccaagctc tttgctgcca tgctctgact gcatccgctc
     2761 cagcatttcg agtagttttt cttctttgtc cgttagtttt gaggtaaata tcgcatcagg
     2821 aatgatagaa gtccaatgaa cattgccttc attatttaat atcatttccg gactgcctgc
     2881 ctgataggac gggcttgctc catttctatg aacatgcaaa ataatttcat gatcatagac
     2941 aggctcaatt cttccctcat ggttacgtac catacgttca ccttgtatgg cgcgatgtaa
     3001 aaccatcagt gtttcttcac taccccatac aaactctctt cgaagatgct ctctgtattc
     3061 atcaagatgt ttttcattat cagctaataa aaaatctatc gtttcttttc ttagaatatt
     3121 atctaaaata gtcgcttgat gaacctggtt ctctttttca gttaaacttt gttctttgag
     3181 ttccagtttt ttattttccc attgacgatt gaattcttct atgacagaag gtgttcttgt
     3241 taaaatatac tcctgaagag catccaacaa accactgacc ttatatatct cagcctcagt
     3301 ataatcagga tttgagaaat cattatctat taacagcgtc aactccgatt cattgatttg
     3361 tagtgagcgt ttaaagccaa attccacagc ataatggaga gatgaaaata aaggcgtatc
     3421 atgaggtgaa gatttaaact cgactttggt atgttccgct gccaaatccc tggttgccct
     3481 gccaaggatg ggttctataa ttgttttgat ttcctcgcgc gtaaacgccc tatcggggtc
     3541 tttggataat agttttcgta aactgatcct atcttcttcc tttaatccta atttgttaaa
     3601 aatatcttca gtaattctga cattattttt tgcgcgcaaa taatacatta atgaaatact
     3661 gtaagcataa tacaagcaat tgcctttacc actattatcc acattaaaca tattgactcc
     3721 tttttgtaaa ttcaaaaccc tcaccacatt aatagtcttt tattgctttt cctccttggt
     3781 ttgatttcat tatttgaaag attcatgagt tcttttctgg taatggactg atactcagtg
     3841 atataagtga gtaaattgat aaatagttta tttgatacga aaagaattcc tttaggggtg
     3901 gattcggagt cattcatttg aacccggtga ccagtgaaat tggtttgcag cggggtatta
     3961 tgatttatga ttaaatccca acatattgac acaacattaa tcccttagtt tttatttgat
     4021 ataaaatgca atgtatacga tcaaaatgat tcacgcaata ctttgatctc attttccgat
     4081 cagtgagtgc gattaatgcc aaagtgattt ttatatgtaa atcattgggt taattcacac
     4141 ggaaaaattg gctataaaat taactgtcgt taacaggtgt tgacaggttt accatagtcc
     4201 tgtatacata atatacaaat taaa
//
LOCUS       DASLOE010000212         4142 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.213, whole genome shotgun sequence.
ACCESSION   DASLOE010000212 DASLOE010000000
VERSION     DASLOE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.213"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            157..897
                     /gene="ravK"
                     /locus_tag="U6309_002711"
     CDS             157..897
                     /gene="ravK"
                     /locus_tag="U6309_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavK"
                     /protein_id="HEN5506125.1"
                     /translation="MVSLEHIQKLISECRKLGEDGLDNGTNGLIPELEIDVVPPSAFL
                     GVGNNPAIFVNSKTYKLMRTTHEKWVENKTIVFKSYLLSQPAIKIIGAIVHETGHAFN
                     VAAKIPNTEANACIFEIEVLMRLFQVKSPLLLGCTELDMQSYFKSRLADYNKCVKDCR
                     CLAEMVQLITHQFKLDEVSISEKENQIPLLSISNKWPGLFAKKQVAHDMDKLLTSPVT
                     ITPEVKILFYQLIKERFHSPETEIKLGI"
     gene            complement(999..2462)
                     /locus_tag="U6309_002712"
     CDS             complement(999..2462)
                     /locus_tag="U6309_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN5506126.1"
                     /translation="MKTNIPTLSKPIKDSGYRRGLKDRHVQLIALGGIIGSGYFLGTG
                     EVINLVGPSVFIAYLLGGLIIYLTMLCMGELAVAIPISGSFVTYTSDFISPTVACGVG
                     WSYWITWVAYIPAECVAGGIIMELFTGVSGYIWVVCFGLIITYINLAKVDTFGEIEFW
                     LALIKIISLLAFVFLAILIFFGLIHGSEPPGIIGFKYLLGDGGLLPNGAMSLLTAMVL
                     LLVNYQGSEIIGLAAGESENPARMIPHAIRNVTFRILFLYIIPVFCLVLIFPWQKAGL
                     SNSVFADALNFYGLKWAGAVTSFVTLSATLSCANSGFYGAVRSLNALARDGMAPHVLA
                     KFNQNSVPQNAVIATLIGVWILLGVGYFFGQTKLYIALLLVSGFTGTLAWLSLCVAQI
                     SFRNRLYKAGYSIKDLRYVTPYSPYTGILAVILMVGSLFFLLLNKDPIYKLSFIIGVV
                     SFIIPVIIYKVFDLSKVRKKALHVKSRVKFQDLFPPQ"
     gene            2836..3981
                     /locus_tag="U6309_002713"
     CDS             2836..3981
                     /locus_tag="U6309_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HEN5506127.1"
                     /translation="MLRWIVLICISLFLPLSAYADKSDCINHHCIAVIDAGSSSSRLH
                     IYAYDLDETNTPIAIREIWNRKAVPGLSTIEHENKSVSAYMEKLFSDAPAVLMPVYFY
                     STAGMRLLPKTHHRNVNQLVAEWFDRQYYWRLVTARTISGTEEGIFAWLATNYQLNLF
                     QQESQTLAGVMDIGGASVQIVIPVVNPSFVDHEHTTTIRLYGKEFTVFSHSFLGLGQN
                     EMGHQFFDLKFCFPEEYELPSGRQARGDAYACKDEIAILVNSVHSVSRKIKSVLAANP
                     IEKWYILGGITFMLNDKLFSLDNQYFTNRDLISFADQNICHQKWSTLEVLYPDNLMVF
                     NYCMLPSYLYALLVHGYGLKSYQKVNFMPNSKNIDWTLGVVLQHKQS"
     gene            3998..4070
                     /locus_tag="U6309_002714"
     tRNA            3998..4070
                     /locus_tag="U6309_002714"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4031..4033,aa:Thr,seq:cgt)
BASE COUNT         1336 a          753 c          806 g         1247 t
ORIGIN      
        1 ccttaaagag aaaagtatat tcatataatt cataaaaaat cttgactgaa taatttcaat
       61 accaaataat ggtctgatgt gaggcatatt ttgatcagtt tgctatcttt gatttcttta
      121 tgatcgcagg gatttcttac aataaggagg ggtattatgg taagtttgga gcatatacaa
      181 aaacttattt cggaatgtag gaagttgggt gaagatggac ttgataatgg tactaatggg
      241 ttaatccctg aattggaaat cgatgttgtc cctcccagtg cgtttttagg ggtaggtaat
      301 aatccggcaa tttttgtaaa ttctaaaaca tacaagttaa tgcgtacaac tcatgaaaaa
      361 tgggttgaaa acaaaacaat agtttttaaa agttatttgc tttcccaacc ggcgatcaaa
      421 attatcgggg ctattgttca tgaaactggt catgctttta atgtagctgc caaaattccc
      481 aatactgaag caaatgcctg tatttttgaa attgaagtgt taatgaggtt gtttcaagtt
      541 aaaagtccct tgttgctagg gtgtacggaa ctggatatgc aatcttattt taaatcacgg
      601 ttagctgatt acaacaaatg tgtgaaagac tgccgatgtt tagctgaaat ggtccaatta
      661 ataacccatc aatttaagct ggatgaggtg tcaatttcag agaaagaaaa tcaaatcccg
      721 cttctgtcta tttcaaacaa atggccaggt ttatttgcaa aaaaacaggt tgctcatgac
      781 atggataaat tattgacttc tcctgtgact atcactccag aggttaaaat cttgttttat
      841 caattgatta aagagcgttt tcattctcct gaaacagaga taaagcttgg tatatagcaa
      901 tatttttgat tgatgcaggt aattactgtg attttatgag tgaggcgtgc aaaatgaatt
      961 ctggcacgcc cagaaatact tcaaaaataa tgtttaattt actgtggcgg aaataaatcc
     1021 tgaaatttca ccctgctttt cacgtgcaaa gcttttttcc taaccttgga taaatcaaag
     1081 accttataga tgatgacggg tataatgaag ctcaccacac caataataaa tgataattta
     1141 tagatgggat ctttatttag cagtaagaaa aataaggagc caaccatcaa aataacagca
     1201 agaattccag tataaggtga ataaggtgtt acataacgga gatctttaat ggagtaaccg
     1261 gctttataca aacggtttct aaagctgatt tgagcaacgc ataaagaaag ccaagccagc
     1321 gttccggtaa aaccggatac taataataaa gcaatataga gtttcgtttg accaaaaaag
     1381 taacccacac ccaaaagaat ccaaactcca atcagtgtgg caataacggc attctgagga
     1441 acagagtttt gattaaattt ggctaaaaca tgtggggcca tcccatcacg tgccaaagca
     1501 tttaaagaac gcacagcacc ataaaatccg gaattagcgc aggataaggt tgcagacaga
     1561 gtgacaaaac tcgttactgc tcctgcccat ttcaggccgt agaaattaag cgcatcagcg
     1621 aagactgagt tggataaccc agccttttgc cagggaaaaa ttaaaaccag gcaaaatact
     1681 ggaatgatgt agagaaacag gattctgaaa gtaacgttac ggatggcgtg agggatcatt
     1741 cgggcagggt tttcagattc tcccgctgca agtcctatga tttcagatcc ttggtaatta
     1801 accaacaaaa gtaccatggc tgtcaacaac gacattgccc cattaggtaa cagacctcca
     1861 tcgcctaaca ggtacttaaa tcctattata ccgggtggtt ctgatccatg aatgaggcca
     1921 aagaaaatta gtattgccag gaaaacaaag gcgagtaatg agataatttt aattaaggcg
     1981 agccagaatt caatttcacc aaacgtatcg actttagcaa ggttaatata ggtaataatt
     2041 aatccgaagc ataccaccca tatgtaacca ctcaccccag taaacaattc cattatgatc
     2101 cctcccgcaa cacattctgc ggggatatag gcgacccagg taatccaata tgaccacccg
     2161 acaccgcaag cgacagttgg ggatataaaa tcagaagtgt aagtaacaaa agagccggaa
     2221 attggaatgg cgactgctaa ttcacccata catagcatgg ttaggtaaat aattaaccca
     2281 ccaagtaagt aggcaataaa cacagagggg ccaacaagat taattacttc accggttcct
     2341 aaaaaatatc ctgagccgat aattccacct agtgcaatca gttggacatg acgatctttt
     2401 aaacctcgtc tgtaaccact gtctttaatg ggttttgata gggttggaat attagtcttc
     2461 acgggctgtt ttttctcact atgttagtaa aaaataatag agctaatgtt atatttatcc
     2521 atataaattt aaaactgctc cagattaaaa aatatttttc atcttattga taactctcat
     2581 ttttgctata tggaagtagc tgataatgac gccccaaaat gaaaaatttc gcgtttatag
     2641 cataaatttt gaacttttaa aagaaacaat ttcattaatt ttttatcttt cttcattttg
     2701 atggaaaaat gagtttcaca ttgatatgga tttccaggtg tctcgctttt attcgcaaat
     2761 tcagttcctg ttttgaccaa aaaaaacaat taatgtagga taaatgcgtt atttgaggtg
     2821 aattaaggaa agactatgtt gcgatggatc gtgttaattt gtatctcttt gtttctgcct
     2881 ttatctgctt atgcggacaa atccgattgt atcaatcatc attgcattgc ggtaattgat
     2941 gctggaagtt ccagttcacg attacatatt tatgcttatg atttggatga aacaaacaca
     3001 cccattgcaa ttagagagat ttggaaccga aaagccgttc ccggattgtc tacaattgag
     3061 catgaaaaca aatcggtttc agcctacatg gagaagctat tttcagatgc tccagctgtt
     3121 ttaatgccag tttattttta ctccacagca ggcatgagat tgttacctaa aactcaccac
     3181 agaaacgtca atcaattggt agctgagtgg tttgacaggc aatattactg gcgacttgta
     3241 actgcaagaa cgatttctgg tacagaggaa ggtatttttg cctggctcgc aaccaattat
     3301 caactaaacc tgtttcaaca agagtcccaa actttagctg gtgtgatgga tattggcgga
     3361 gcatctgttc agattgttat tcctgttgta aatccctctt ttgtggatca tgagcataca
     3421 accacaattc gcctctacgg caaggaattt acggttttct ctcatagttt tcttggtctt
     3481 ggtcagaatg aaatgggcca tcagtttttt gatttaaaat tctgctttcc agaggaatac
     3541 gaactgccaa gcgggcgcca agctcgaggc gatgcctatg cttgcaaaga tgaaattgcc
     3601 attttagtca atagcgttca ttctgtgagc cgaaagatta aatcagtttt agccgctaac
     3661 cctattgaaa aatggtatat tttgggcggc attaccttta tgttgaatga caaactgttt
     3721 tctctggaca atcaatattt taccaatcgt gatcttattt cctttgcaga tcaaaatatt
     3781 tgccatcaaa aatggtcaac tcttgaagtg ctctatcccg ataatctcat ggtgttcaac
     3841 tactgcatgc taccttccta cctttatgcc ttgttagttc atggctatgg acttaagagt
     3901 taccagaaag ttaatttcat gcccaatagc aagaatatcg attggacctt aggagtggta
     3961 ttgcaacata agcaatctta agtatacttt tctttatgcc gttgtagctc agttggtaga
     4021 gcaattgatt cgtaatcaag aggtcggagg ttcgactcct ctcagcggca tggtaaaatc
     4081 aatgagttac aaatagaaag ttaaaaattg aaaaaatttg gtaccgtttt tggtaccagt
     4141 tt
//
LOCUS       DASLOE010000213         4084 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.214, whole genome shotgun sequence.
ACCESSION   DASLOE010000213 DASLOE010000000
VERSION     DASLOE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.214"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..879)
                     /locus_tag="U6309_002715"
     CDS             complement(<1..879)
                     /locus_tag="U6309_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEN5506128.1"
                     /translation="MHQKKRSSLELEALLSLFEGSLRTIPFNILAVIVLALGFIYTNE
                     VPIKLIIIWFFLILILSVIRWVTSRIIISKEFYTTKGKQSLIGFLLLNFLMGLAWSLS
                     YFIFSPYLNNTNELIFVLILGGLSTGAIASLSIYLPAYYAYVLPMNFPIIAYNLYLFQ
                     FDKIIFAAMYSLFLIIVLLTARLNSKLLSKVRALSITDSLTGLFNRRHFDVVFNNELS
                     RAKRNKYPISLVFIDIDNFKYINDTFGHSAGDSFLIHVSNILKQTLRRSSDSMFRIGG
                     DEYAAILINMTPNEVTT"
     gene            complement(1022..1165)
                     /locus_tag="U6309_002716"
     CDS             complement(1022..1165)
                     /locus_tag="U6309_002716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506129.1"
                     /translation="MEFDYIIFGDGSPGWVLLPGLSANPANKLLLLEAGDSVFLEMLI
                     CPQ"
     gene            complement(1303..1713)
                     /gene="fur"
                     /locus_tag="U6309_002717"
     CDS             complement(1303..1713)
                     /gene="fur"
                     /locus_tag="U6309_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEN5506130.1"
                     /translation="MEESQQLKDAGLKITLPRIKVLQILEQSRNHHLSAEAVYKALLE
                     SGEDVGLATVYRVLTQFEAAGLVSRHNFEGGHSVFELSQGEHHDHLVCVKCGRVEEFV
                     DEIIEQRQKAIAERAHFKMTDHALNIYGICPQCQ"
     gene            1911..2255
                     /locus_tag="U6309_002718"
     CDS             1911..2255
                     /locus_tag="U6309_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEN5506131.1"
                     /translation="MAKFFMVGKKMRIMTFFICFILTLTLTQCASYDFSRRITQQGNL
                     LPQSKIARLKTGMSKNDVAILMGTSLMSPTFNNDRWDYAYTWRRGKGNLEIRNVVLYF
                     SQGSLVKIEHKP"
     gene            complement(2343..2615)
                     /locus_tag="U6309_002719"
     CDS             complement(2343..2615)
                     /locus_tag="U6309_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEN5506132.1"
                     /translation="MVKVELVYIANSKNCLHYKMNLKQGATVEEALIESNIYSTCPET
                     RELPVGVYGKRVSVDQVLKEGDRIEIYRPLILDPKEKRRKLAQFKK"
     gene            complement(2602..3036)
                     /locus_tag="U6309_002720"
     CDS             complement(2602..3036)
                     /locus_tag="U6309_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEN5506133.1"
                     /translation="MTIVKRSRTVPYSCEQMYGLVNDVEHYSEFLPYCAESKILHRDN
                     DEVQATLVIAAAGMSKSFTTRNRLQTNKMIEIRLVDGPFSHLEGFWRFDQEEKGCRIS
                     FDLEFEFAGKIFSMLLGPIFDQITDKMVDAFCERAEVIYGKG"
     gene            complement(3036..3680)
                     /locus_tag="U6309_002721"
     CDS             complement(3036..3680)
                     /locus_tag="U6309_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEN5506134.1"
                     /translation="MKEPLEKAQACVIWMHGLGADASDMMGLADQLTIEDTALRHVFL
                     DAPRRPVTLNGGMVMPAWYDIYGLGFVDEEDKSGIEQSELLIRKVVDAQYNCGFKPHQ
                     IFLAGFSQGGAMALHTALHMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGAGQFD
                     PLVLPKWTLQSKDWLLANGYNEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVL"
     gene            3870..>4084
                     /locus_tag="U6309_002722"
     CDS             3870..>4084
                     /locus_tag="U6309_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506135.1"
                     /translation="MSILFLARKLASDYGFIEQIQQSHNRNQINYRFYSPRTDLHGSN
                     TNRLYGNPGKGVISQNTIFIYWYRDGK"
BASE COUNT         1354 a          837 c          665 g         1228 t
ORIGIN      
        1 tgttgttact tcatttggtg tcatattaat aagaatggca gcatattcat caccaccaat
       61 ccgaaacatg ctatcgctgg agcgccgtaa tgtttgtttc aggatatttg acacatgaat
      121 taaaaaactg tcgcctgcag aatggccaaa agtatcatta atatacttga aattatctat
      181 atcaatgaaa accaggctga taggatattt attacgtttc gctctgctta gttcattgtt
      241 gaacaccaca tcaaaatgac gacggttaaa taacccggtc aatgaatcgg ttatagataa
      301 tgccctcact ttagaaagta attttgagtt taatcttgct gtcaacaata ctatgatcaa
      361 aaaaagcgaa tacatagcag caaaaattat tttatcaaac tgaaataaat acaaattata
      421 ggcaataatt gggaaattca ttggcaatac gtaagcataa taggcgggaa gataaattga
      481 taaagaagcg atagcgcctg tcgataatcc ccccagaatg agtacaaaaa tcaactcatt
      541 tgtgttattt aaataaggag aaaaaataaa gtagcttaaa ctccaagcca gccccattaa
      601 aaaattcagc agtaaaaaac caattaaaga ctgctttcct tttgtggtgt aaaactcttt
      661 actgattata attcgactag ttacccaacg aatcacactt aaaattaaaa tgagaaaaaa
      721 ccagataata atcagtttga ttggaacctc gttcgtatag ataaaaccca aagcaagtac
      781 tatcactgcc aggatattga atgggatagt tcttaaggat ccctcaaata atgacagtaa
      841 ggcttcaagt tccaatgaag accgcttctt ttgatgcata attattcaaa acccaagcac
      901 cttcattata atttcaagta tagactagtt tagagagttg ctctttaccg cccaaattcc
      961 atttagaata ggaggctctc acgaagaatt ggaatatagt gtgataattg ttctacaatt
     1021 ctcattgcgg gcagattagc atttccagaa atacgctatc tcccgcttcc agcaacaata
     1081 atttattggc gggattggca cttaaacctg gaagcaaaac ccatcctggg ctaccatcac
     1141 caaaaataat ataatcaaat tccatgtgat gaatccttaa agaaaatagc atttcaccaa
     1201 aacgctctga tcattttaat gataaagcac tacaaaggaa tatagtaaac acgctgagga
     1261 tattccgtga agtgtcaagc caatccagtt aaaatatcaa agtcattggc actgcggaca
     1321 aataccatat atgtttagcg catgatctgt cattttaaaa tgagcgcgct cggcaattgc
     1381 tttttgtctt tgttctatta tttcatctac aaattcctct acccgcccac acttaacaca
     1441 aacaagatgg tcatgatgtt cgccttgaga caactcaaat accgagtgtc ctccttcaaa
     1501 attatgcctc gatactaatc ccgctgcttc aaattgagta agaactctat acacagtagc
     1561 taaaccaaca tcctcacccg attccaataa ggctttatag actgcttctg cactcaaatg
     1621 atggtttctt gattgctcca atatttgcaa taccttgata cgaggtaatg tgatttttaa
     1681 tccagcgtct tttaactgtt gactctcttc cacttgtgct ccttaactgt aacgaatttc
     1741 gagctttgat cattattcca gcaaaattgg attaataact agcatcaagg cctaaagtaa
     1801 ttttcatgct ctaaaaacca ttagcggcct taaaacttag tctggccgaa taatggcaaa
     1861 taccaaattc cacatgatga caggtgcctt tcaataaatg acatgttatt atggcgaaat
     1921 ttttcatggt aggcaaaaaa atgcgaataa tgactttttt tatttgtttc attttaacat
     1981 taaccttaac ccaatgtgca tcatatgact tctcaaggcg catcactcag caaggtaatt
     2041 tgttgcctca atccaaaatt gcccggttaa aaacaggcat gagtaagaat gacgttgcaa
     2101 tcttgatggg taccagctta atgagtccaa cattcaacaa tgataggtgg gattatgctt
     2161 atacctggcg gcgaggaaaa ggtaatctgg aaattcgtaa tgttgtgtta tatttttccc
     2221 aaggctcttt agtaaagata gaacataagc cgtaatttaa tatctccccc ttgcctaagt
     2281 gattagacaa ggggaaagtt gtttttaaga gttcagtgta gattaaaatg cctaatatgc
     2341 ctctattttt taaactgtgc taacttacgg cgtttttctt tggggtcgag tatcaatggc
     2401 ctataaatct caattcgatc tccttctttc aatacctggt ccacagaaac ccgcttccca
     2461 tatactccta caggtaattc cctcgtttca ggacaagttg aataaatatt tgattcaatg
     2521 agtgcctctt caactgtagc tccctgtttt aaattcattt tgtaatgcag gcaattttta
     2581 gaattcgcta tatagactaa ttcaaccttt accataaatg acctcggctc tttcgcaaaa
     2641 tgcgtcaacc attttatcgg ttatctgatc aaatatagga ccaagaagca tggaaaatat
     2701 tttcccggca aactcaaact ccaaatcaaa agaaattctg cacccttttt cttcttgatc
     2761 aaaacgccaa aacccctcta aatgactaaa aggaccatca accagacgaa tttcaatcat
     2821 tttattagtt tgcaatcgat tacgagtagt gaatgactta ctcattccag cagctgctat
     2881 caccaaagtc gcctggactt catcattgtc acgatgaagt attttgcttt ccgcacaata
     2941 aggtagaaac tctgaataat gttcaacatc attaaccagt ccatacattt gctcacaaga
     3001 ataagggact gttcttgacc tttttactat tgtcattata aaactccttg aacctgctta
     3061 ttaagccaaa ggctaagatc ttttatttct tcaaaacata tggaatgttc catggggtat
     3121 tgatgaaaag agacttcatt atacccatta gccaagagcc aatccttact ctgtagagtc
     3181 cattttggca gaactaaagg atcaaattgt cctgctccca taaagatagg agtattctta
     3241 tcgagttgcg gcttattatg cttagccaag ggcaaataag cggacaatgc aataacgccg
     3301 cataatcgct cagtcatatg aagagccgtg tgcaatgcca tagctccccc ttgagaaaag
     3361 ccagctaaaa aaatttgatg aggtttaaag ccacaattat attgagcgtc aaccaccttg
     3421 cgtattaaca attcagattg ctcaatgcca gacttatctt cttcatcgac aaagcccaat
     3481 ccataaatat cataccacgc aggcataacc attcctccat tcagagtcac tggtcttcga
     3541 ggagcatcaa gaaatacatg ccttaaagcg gtatcttcta ttgttagctg atcagctaaa
     3601 cccatcatat cagaggcatc agctcccaac ccatgcatcc atatgacaca agcttgtgct
     3661 ttctctaaag gttccttcat ataaacgctc aaaacaaatt ctccacgcaa aaacgtaaat
     3721 tatcgccaat gcaatagagc agcgcaagtt taacatttaa aaatatattg aacaatttat
     3781 agacatattg acgctgggaa ttctaataac tattgacttt attgtgtaaa tatttatcat
     3841 tatgtttgtt ttatatgtta tgctgtttta tgagtatttt gtttttagca agaaaactgg
     3901 cttctgacta cggattcatt gagcaaatcc agcaaagcca taatcgtaac caaataaatt
     3961 atcgctttta ttccccacga actgatctgc atggttccaa tacaaacagg ttatatggaa
     4021 accctggcaa aggtgtcatt tcacaaaata cgattttcat atactggtac agagatggaa
     4081 aaaa
//
LOCUS       DASLOE010000214         4037 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.215, whole genome shotgun sequence.
ACCESSION   DASLOE010000214 DASLOE010000000
VERSION     DASLOE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.215"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..993
                     /gene="mavA"
                     /locus_tag="U6309_002723"
     CDS             <1..993
                     /gene="mavA"
                     /locus_tag="U6309_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector MavA"
                     /protein_id="HEN5506136.1"
                     /translation="REQLKQFTLDDFNDISGFERKEKSNSRQQSYFKLRQELEFFLKN
                     DIQQHKSCPDAQLNAFRRWINISDLLLRHHCYEGFLLVFVNLQLIADKQLIDGLPVSV
                     RNNYNQLCQLSSPIGNHGALRHFMNTHQGESDFTPLFFTYHAIGALDESLESLKDKEV
                     LLKKQLKHLNKKISHLRTEATPGVIDVISELLKNKQQIPKKMMERRGHLIQLMEEVRF
                     TGKQLKQIQVNMQNQLEQRANLVGLIVKEQKATPRTLPEYFEKTYSIIKYRFNRQSIA
                     TVKSSQLPESTASSNPSSSNLYKSKLLPHFWNRHGKSSSSYWEEVFTPSCLNNK"
     gene            complement(1044..1361)
                     /locus_tag="U6309_002724"
     CDS             complement(1044..1361)
                     /locus_tag="U6309_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506137.1"
                     /translation="MKIVSAYVLRLFAILNLTFLLFITISFTNVASASNTNADQYLSG
                     NTQLAYFIGVHSYPGNYYWRGPVYYKNAYWTKWRYIGYGCQKSCLIDRWSGRVIRCAK
                     RCF"
     gene            1580..2449
                     /locus_tag="U6309_002725"
     CDS             1580..2449
                     /locus_tag="U6309_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1685 family Dot/Icm T4SS effector"
                     /protein_id="HEN5506138.1"
                     /translation="MQCAIERKNSLRDLQSQPNLGDWMFISSLGSQDFDERKFFSQIS
                     LQSLSFIIRIAKFSAYFAALCEKSEYDELWKKLYSTFGLVLTRDKPVPKSFFTHDGGE
                     INHFNLLRGAYYFHLSQKALAAKEKSFSDSELYWLNQAMKFGSIHASQRYIQFLYQKL
                     DKVASADEAEQILTEAIKLCKANLNQYGSYAYMMLAEAFFRYAMWAQQSGNINRAKSA
                     ITSSINSCTKANQHLRASASSIHNASLGEGLKASNTLAMESPEEAIAFLNAWAVAHLY
                     KHEIELVSRPGFY"
     gene            complement(2460..3857)
                     /locus_tag="U6309_002726"
     CDS             complement(2460..3857)
                     /locus_tag="U6309_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1684 family Dot/Icm T4SS effector"
                     /protein_id="HEN5506139.1"
                     /translation="MKSVDEIDSLLQSAKTSEKDSSEQIVPPNDEIEDGFYLIDDSNH
                     DEDLDFSDFPWLDPDHPLFFPATQANNSKGSTRWDAVNKFSVENWYPHLRKHTFKSHF
                     ITLNYNDIHYLMGNIPPDYDSSKLESIFNKILSEFNNKEVFMRLSTRSPKDSKFLFEE
                     ASTIMSKDFVYWNENDNKHQQLVSFVASMLKAMKIKSGRKIIETIAQSPRVYNDLLAL
                     VSSSNQSDCTTNVILREWHDIRPDHEFRVFVSRRHRKESIVTAISQYFHFLYFDKSPA
                     DCFNFLDEEDKKAVIKKFENYVLKSVDPDVAKFLNFSSEQDDDESSDCIREYIVDLAL
                     IPVSQYHGEITDENIIEIGANTYVMVVIELNPFAPAATGSGLFNWKNDLMMLWGKASC
                     DYPVFKYRTTPREDLQSVSLLPSNYESVIKSALIKRLETSYSNSASQKERFFSSTKPV
                     TESSGSALLDMVTKP"
BASE COUNT         1316 a          716 c          709 g         1296 t
ORIGIN      
        1 agagagcaat tgaaacagtt tactctggat gattttaatg atatttcagg atttgaacga
       61 aaagaaaaat cgaattccag gcaacaaagc tatttcaaat taaggcaaga gctggaattt
      121 tttcttaaaa atgacattca acagcataaa tcttgccctg acgctcaatt aaatgcgttc
      181 agacgctgga taaatatatc ggatttactc ttaagacatc attgttatga aggctttctt
      241 ttggtatttg ttaatttgca attaattgca gacaaacagt taatcgatgg cttacctgta
      301 agcgtaagaa ataattataa tcaactatgt caattaagtt caccaatagg gaatcatggc
      361 gctttgcgcc attttatgaa tacgcatcag ggtgagagtg attttactcc tttatttttt
      421 acctatcatg ccattggagc tttggatgaa tcgcttgaat ccttaaagga caaggaagtt
      481 cttttaaaaa agcaattaaa acatctgaat aaaaaaatta gtcatttaag aacagaagcc
      541 actccaggag tgattgatgt catttctgaa ttattgaaaa ataagcaaca aataccaaaa
      601 aaaatgatgg agcgtagagg acatctgata caactgatgg aagaggttag attcactgga
      661 aaacaattaa aacaaataca agttaacatg caaaaccaac ttgaacaacg cgctaatttg
      721 gtaggattga tagtcaaaga acaaaaagca acgccaagaa cgctccctga gtatttcgaa
      781 aaaacctata gtattatcaa gtacagattc aataggcagt ctattgctac agtcaaaagc
      841 tctcagcttc cagaatcaac agcaagcagc aatccaagtt cctcaaattt gtataaaagt
      901 aaattgctcc cccatttttg gaatagacat ggaaaatctt cctccagtta ctgggaagag
      961 gtattcacgc caagttgttt gaacaataaa taagagaatt agatgaactc tgcttcatac
     1021 aagccctgtt tatatgaatt tggttaaaaa caacgtttgg cacagcgaat gactcgacca
     1081 ctccatcggt ctatcaggca gcttttctgg caaccataac caatatatct ccattttgtc
     1141 cagtaagcgt ttttgtaata tacggggccg cgccagtagt agttaccagg gtagctgtga
     1201 accccaatga agtaagcaag ttgagtattt ccggaaagat attgatcagc attagtgttg
     1261 ctggcacttg ctacattagt aaatgatata gtaatgaaca atagaaaagt taagttcaga
     1321 atagcgaata atctcaggac ataagcactt actattttca tgataaattc cttttatcgc
     1381 agataatatt caaattatag attaataata caacaaattg tctcataata tttcaaaatt
     1441 gcttttttac ttaagttgct tgttatgtat tttaggaggt atataaaaat tgtattaagt
     1501 catgtgtgca atattatgta caatatgtta atatattgcc atgcttgtta tttggcaaag
     1561 atgttgaggg cttaaaaaat tgcaatgtgc tatcgagaga aaaaatagtt tacgtgattt
     1621 gcaatcacag ccaaatcttg gtgattggat gtttatctcc tctttaggga gccaagattt
     1681 tgatgagaga aagtttttct ctcaaatttc attgcaatca ctgtctttta ttatcagaat
     1741 agctaaattt tctgcttatt ttgctgcgct ttgtgagaaa agtgaatatg atgagctttg
     1801 gaaaaagctc tactcgactt ttggattagt tctcaccaga gataaaccag tacctaaatc
     1861 tttttttact catgacggtg gagaaatcaa tcattttaat ctactacgtg gcgcttatta
     1921 ttttcatctt tcccaaaaag cattagcagc aaaagaaaag tccttttctg actcagagct
     1981 ctactggtta aatcaggcaa tgaaatttgg gtcgatccat gcaagccagc gttatatcca
     2041 atttttatat caaaaattag ataaggttgc ttctgcagat gaagcagaac aaattttaac
     2101 ggaggcaatt aagctttgta aggcaaactt aaatcaatat ggcagttatg cttatatgat
     2161 gctagctgaa gctttttttc gttatgcgat gtgggcacaa caatccggta atataaatcg
     2221 tgctaaatct gcaattacct cgtcaattaa ttcctgcaca aaagctaatc aacacctgcg
     2281 tgccagtgcc tcctccattc ataatgcaag cttgggggag gggttgaaag ccagcaatac
     2341 acttgctatg gaaagcccag aggaagccat cgctttttta aatgcttggg cagtcgcgca
     2401 cctttataaa catgaaatag aattggtctc ccgcccaggt ttttactaaa tcttgcctat
     2461 cacggttttg taaccatatc aagcagggct gatccagagg attcagtaac aggtttagtg
     2521 gatgaaaaaa atctttcttt ttgacttgct gagtttgagt aggaggtttc caatcgtttg
     2581 atcaaggcac ttttgataac agactcataa tttgacggca gcaatgatac tgactgtaaa
     2641 tcttctcttg gtgttgtacg atacttaaag acgggatagt cacaggatgc tttcccccaa
     2701 agcatcatta agtcattttt ccaattgaat aaaccacttc ctgttgctgc tggtgcaaat
     2761 ggatttagct ctatgactac cattacataa gtatttgccc ctatttcaat tatgttctca
     2821 tctgttattt cgccgtgata ttgactgact ggaatcaagg ctaaatcaac aatgtattca
     2881 cgaatgcaat ctgatgattc gtcatcatct tgttcgcttg aaaaatttaa aaattttgca
     2941 acatctggat ctacagattt gagaacataa ttttcaaatt ttttaataac agctttctta
     3001 tcctcttcat ctagaaaatt gaaacaatct gcagggcttt tatcaaaata taaaaaatga
     3061 aaatattgtg atatcgctgt aacaatactt tctttacgat ggcgtctgga tacaaaaact
     3121 ctaaactcat ggtctggacg aatgtcatgc cattcgcgca aaataacgtt tgtcgtacaa
     3181 tctgattgat tgcttgaact aacgagcgca agcaaatcat tataaactct agggctttgt
     3241 gctattgttt caattatttt tctaccactt ttaattttca ttgccttgag cattgaagca
     3301 acaaatgaca ccaattgctg atgtttattg tcattttcat tccaatacac aaaatcctta
     3361 ctcattatgg tagaagcttc ttcaaagaga aatttggaat ccttggggct cctggtggac
     3421 aatctcatga agacttcttt attgttaaat tctgaaagga ttttattgaa aatactttcc
     3481 agtttactgc tatcgtagtc tggaggaatg tttcccatta aatagtgaat atcattatag
     3541 tttagtgtaa taaagtgtga cttgaaggta tgttttctta aatgaggata ccaattttct
     3601 acagaaaatt tatttaccgc atcccatcgt gtcgatcctt tcgaattatt tgcctgtgtt
     3661 gccggaaaaa ataatggatg gtcaggatcc agccatggaa aatcactaaa gtcaaggtct
     3721 tcatcatggt tactatcatc tatgagataa aatccatctt caatctcatc atttggtgga
     3781 acaatttgct ctgaactgtc tttttcactt gttttggctg actgcaaaag agagtctatt
     3841 tcatcaacag atttcatgtt taccgcactc cacccaacgc tttgtaaatt taatattcac
     3901 accataatcc aataacatga cttattagga tacaatatgg ccaattaatt ctaacacttt
     3961 ccgctgtaat tgccaaatac cgagccacca tgaattaaat ttaatgatta tgtaagaact
     4021 taataatgaa atgatta
//
LOCUS       DASLOE010000215         4021 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.216, whole genome shotgun sequence.
ACCESSION   DASLOE010000215 DASLOE010000000
VERSION     DASLOE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.216"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..147)
                     /locus_tag="U6309_002727"
     CDS             complement(<1..147)
                     /locus_tag="U6309_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5506140.1"
                     /translation="MHTKKDKKVISLQERVENAVDVSGAFDNCFFHNFALYLLTNNLP
                     LPDDL"
     gene            complement(267..1025)
                     /locus_tag="U6309_002728"
     CDS             complement(267..1025)
                     /locus_tag="U6309_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506141.1"
                     /translation="MNLVQRMIPFFGLVILAIYSTSSMALSVELIETSPKSPVTLNQD
                     EALYVLIRYKSDQPLRFQAIGENLQQKIMDSARFNPSQAYPAGEGEAIAWVAYDNTTE
                     IDSITVTIYDANWRALQTKSIPVSAVWQNENGRNNQAAAPWVQRLNQQQQSSVFTQSQ
                     TPVSSGNALFVQLLFLLILLYWFLQIIVIFNWTGRWKKLACFPLLFSVPLLLYTLYAL
                     YAGSNLWPLMMLFVTPFILAFLLIIIGYKKVYSG"
     gene            complement(1159..2175)
                     /locus_tag="U6309_002729"
     CDS             complement(1159..2175)
                     /locus_tag="U6309_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEN5506142.1"
                     /translation="MYSLDWPRAYGMPNSTATFKSLPEDFQVNELFEGQFSGEGEHIV
                     LKIEKRGLTTEEVVKSLARLINKPVKLISHAGLKDKQALTTQWLSVHAPGEIIDGIET
                     LEAPGWRILECTRHNKKLKPGFLSGNHFIITLRNVSDESDLIHRIEQIKFKGVPNYFG
                     EQRFGRDGGNLIKAEEILVQGRKVKDRFLKGMYFSAARSWLYNLILSRRVKESSWNLP
                     LLGDVIQLVGSNSIFVNDKSMDEQLLQRIGEKDVSPASPLPGRSKNLVKGTALQIIND
                     VYEEWSAWLDGLEKNGLEEAWRANILYAEQIEYRINQGTVELSFVLPAGAYATVVLRE
                     LVQY"
     gene            2468..3451
                     /locus_tag="U6309_002730"
     CDS             2468..3451
                     /locus_tag="U6309_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN5506143.1"
                     /translation="MIVNSSFRLLWWLSNRHAQTLYPTLVKKRLKPRIDFHERLELPD
                     GDFIDLAWSVNELNKNTPLVILLHGLGGGINSIYVSGLMQALADAGFRCVLMHFRGAS
                     EEPNRILRTYHSGDTADFAYFLEILAKREPATKKAVVGISLGGNVLLKWLGETASSLW
                     VDAAVAVSVPFQLNSVADRMNQGFSRLYQAYLLSRLRRIFLKKLNHLNNSPFSKNDLL
                     SLKTFWDFDEQITAPINGFKSAHEYYQKASSRQYLKNISTPTLIIHAQDDPFMTPDVI
                     PTLEELSSQTTLELSEYGGHVGFIAGNGKSSSQPVYWLEQRIPEFLLDYLS"
     gene            complement(3476..3871)
                     /locus_tag="U6309_002731"
     CDS             complement(3476..3871)
                     /locus_tag="U6309_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506144.1"
                     /translation="MQNEKEVKELIQSDKKKRVTWNDNQTEGQIEEKFLKIGTASTLY
                     KNKLDESIAQLMEDGYSEVEATSITGKSVTPRGKVIYNNPVFYKSKESIDIEKANRYN
                     FFSIRNIGLGVVAIAAAAATVGLVVSKNS"
BASE COUNT         1273 a          793 c          664 g         1291 t
ORIGIN      
        1 taaatcatca ggaagtggta aattattcgt taacaggtaa agtgcaaaat tatggaaaaa
       61 gcagttatca aaagcccccg aaacatcaac tgcattttct actctttctt gaagtgatat
      121 tactttttta tccttttttg tgtgcataat ttaatctatc tctttgttat ttgaaacaat
      181 tacattgatt tttgattata aagaatcatt attaagcaaa tattaagaga taaaatataa
      241 taatagatta aattggatgc atcttatcat ccggaataga cttttttata cccgataatg
      301 atcaataaaa atgccaggat aaatggtgtc acaaataaca tcatcaaagg ccataaatta
      361 gaacccgcat ataacgcata aagcgtatac aacagcaaag gtacgctaaa taataaaggg
      421 aaacacgcca attttttcca tctccccgtc caattaaaaa taacaataat ttgcaaaaac
      481 caatacaaaa gaattagtaa aaaaaggagt tgaacaaaca gggcattacc agaggagaca
      541 ggagtttgtg actgagtaaa cacactggat tgctgttgtt gattcagtct ttgaacccag
      601 ggagctgctg cttgattgtt tctgccattc tcattttgcc agactgcaga cacaggaatt
      661 gatttggttt gcaaagccct ccaattggca tcatagattg tgacagtaat ggaatcaatt
      721 tctgtggtat tatcgtaagc tacccaggca atagcctcac cttctccggc tgggtacgct
      781 tgtgatgggt taaacctggc gctatccatt attttttgct gcagattttc cccaatagcc
      841 tggaatcgca aaggttggtc actcttatat cgaataagca catacaaagc ctcatcctgg
      901 tttaatgtaa caggagattt aggactggtt tcgataagct ccacactcaa tgccatggat
      961 gaagttgaat aaatagccag gatcactagc ccaaaaaaag ggatcatcct ttgaaccaga
     1021 ttcatgaatg tgtccttcaa ttaaataaac atttctcttt attatggcat aacatcaaac
     1081 atagtgatta attcataaat tcaattaaaa aataattact ccacattgac taaagtgcta
     1141 ttcaagtgca aacaaagctt aatattgaac caattcacgt aataccactg tcgcgtaagc
     1201 accagccggc aagacaaacg aaagctcaac tgtaccttgg tttatcctat attcaatctg
     1261 ctcagcataa aggatgtttg cacgccaggc ttcttccaaa ccattttttt ctaacccatc
     1321 aagccaagca gaccattcct cataaacgtc gtttattatt tgcaaagctg tcccttttac
     1381 aagattctta ctcctaccag gcagagggct tgctggagaa acatctttct caccgattcg
     1441 ttgcaacagt tgctcatcca tgcttttatc attaacaaat atactgtttg atccaacaag
     1501 ctgtatgaca tcccccaata agggcaaatt ccatgatgat tcctttacac ggcgagacaa
     1561 gatcaaatta tatagccatg atcgggctgc ggaaaaatac attcccttta aaaatctatc
     1621 ctttactttg cgcccctgta ctagtatttc ctctgcttta attaaatttc caccatctcg
     1681 accaaaacgc tgctcaccga aataatttgg aacacctttg aatttaattt gttcaatacg
     1741 atgtattaga tcactctcat cagagacatt tcttaaagtg attataaaat gattacccga
     1801 taaaaaacct ggtttgagtt ttttgttatg acgcgtacat tccagtattc tccaacctgg
     1861 cgcttcgagt gtttctatgc catcgattat ctccccgggt gcatgaacac tgagccattg
     1921 agttgttaat gcctgtttat cctttaatcc agcatgactt ataagtttta cgggtttatt
     1981 aattaacctg gctaatgatt taacaacttc ttcggtagtt aatcctcttt tttcaatttt
     2041 aagaactatg tgctctcctt caccactaaa ttgtccttca aacaattcat ttacttgaaa
     2101 atcttccggc aacgacttaa aagtagctgt agagtttggc atgccatagg ctcgtggcca
     2161 atccaaagag tacatcaaga tctccttcaa caaaaaaggg ttctaaacta aatccacaaa
     2221 ttcactataa gttcacagag atacagaact tattggttga gataatgcat ctctgcgcaa
     2281 aatacctatc ttaactatcg tttgtcattt gatataggga ctataaaaca ttatgatgca
     2341 agattaccaa caaaaattag tttaaattct tgattgacta taatttaaat agatattttc
     2401 ttttgcaatt tctgatcatg atctgcgtta tccagttaga attaaatttt ttattttcag
     2461 gttatatatg attgtaaaca gttcatttag gctcctgtgg tggctaagta acaggcatgc
     2521 gcaaaccctg tatcctactt tagtaaaaaa gcggttaaaa ccgcgaattg attttcatga
     2581 gcgactggaa ttacctgatg gtgattttat tgatctggcc tggtctgtta atgaattaaa
     2641 taaaaataca cccttggtca tattactgca tggtttgggt ggaggaatta actccatcta
     2701 tgtcagtgga ttaatgcaag cattagccga tgccggattt cgttgtgttt taatgcattt
     2761 tcgcggagcc agtgaagaac ccaaccgtat tttacgtacc tatcattccg gtgacacggc
     2821 tgattttgcc tattttcttg aaattttggc aaagagagaa cctgcgacaa aaaaagcggt
     2881 tgtcggcata tccttgggag gcaacgttct actgaaatgg ttgggcgaaa cagcatcatc
     2941 tttatgggta gatgcagctg ttgctgtttc cgtaccattc caattaaatt cggtggctga
     3001 cagaatgaat cagggttttt ctcgcctgta tcaagcttac ttattaagtc gactgcgccg
     3061 tatttttcta aaaaaattaa atcacttaaa taattcgcca ttttcaaaaa atgatttact
     3121 ttccttaaaa actttctggg attttgatga gcaaattaca gcaccaatca atggttttaa
     3181 aagtgcacat gaatattacc aaaaagcaag ctccagacaa taccttaaga atatcagtac
     3241 gccaacttta attattcatg cgcaggatga tcctttcatg acaccagatg ttatcccaac
     3301 attggaagaa ctatcttctc aaaccacttt ggaattaagc gagtatggcg ggcatgtagg
     3361 ctttattgcc ggtaatggaa aaagctcaag ccaacctgtg tattggcttg aacagcgaat
     3421 tcctgagttt ttgttagatt atttatctta aaaaattaat gggaatacaa tacgtttagc
     3481 tgttttttga tacaaccaat ccgactgttg cagcagcagc tgctatagcc actacaccta
     3541 atccaatatt tcttattgag aaaaaattat aacgattcgc tttttctata tcgatgcttt
     3601 ccttagattt ataaaaaaca ggattattgt aaataacctt acccctaggt gttaccgatt
     3661 ttcctgttat tgatgttgct tcaacttctg aataaccatc ttccatcaat tgggcaatac
     3721 tctcatccaa tttgttttta taaagtgttg aagcagtgcc aattttcaga aatttttcct
     3781 cgatctgccc ttcggtctga ttatcattcc aagtaactct ttttttctta tctgattgaa
     3841 tgagttcttt cacttctttc tcattttgca tacaaacctc tctataacca accaaaattt
     3901 aaacaatatt agcaataaat acttaagaaa atatgaactc aaaagatatt ttaaaagttg
     3961 cgcctatttg gaaactcaga tgaaaccgag ttgatcgatg aaattccata tcagactgta
     4021 a
//
LOCUS       DASLOE010000216         4017 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.217, whole genome shotgun sequence.
ACCESSION   DASLOE010000216 DASLOE010000000
VERSION     DASLOE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.217"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(198..1532)
                     /gene="lem17"
                     /locus_tag="U6309_002732"
     CDS             complement(198..1532)
                     /gene="lem17"
                     /locus_tag="U6309_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem17"
                     /protein_id="HEN5506145.1"
                     /translation="MEDESKVKKGLKEEKEQLNQKWLEQLLNNPRWGPSVVKLAMARK
                     KENRNKRGEAFNCFVLSRPQNAFEEDEFRKFLDKLKNNINDFSGTTRFQIALFTADNH
                     HWTAFDFFIVDGKLHVFCLDAANDSAGDAALDEIMSRFPGCDAYRLEPDRDPKAKDSK
                     HFRLIQTDRENCSRMTIEHILLLSKKKFLFNENFANYKVVNGVKLINPDALFRQSPEL
                     YRMTQIDSILDSLMIASPKEAQRLVSKKGLNAEQYRDLLGHTSGKQSKSSKNTTIDYK
                     RQRVEKNLLAFLAESEQKHLEDILDNSEKQWSEFLEMSQRERIQFLLGNIRERPFKEI
                     VGNILGKGIKDRLDHKKYIENDVIKKSSFTLFRGIDKHFEIKKIKAEINELEKALKIV
                     NKLSNDTSADEVLLHLQKQKLPLGIKTELAGALDIYTLLDKEKSALQSHKIN"
     gene            1766..2962
                     /gene="ralF"
                     /locus_tag="U6309_002733"
     CDS             1766..2962
                     /gene="ralF"
                     /locus_tag="U6309_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214205.1"
                     /note="RalF, a virulence factor secreted by type IV
                     secretion systems (T4SS), is a guanine nucleotide exchange
                     factor that acts on host cell ADP-ribosylation factors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T4SS guanine nucleotide exchange effector RalF"
                     /protein_id="HEN5506146.1"
                     /translation="MHPEIEKAQREIIEAFNAKPKNGIKKIKEICGEYKISPNEEIAA
                     FFHQQRKNLDLEAVGDYLSEPEAENQQVLKAFTSQMDFNGQSFVEGLRIFLKTFKLPG
                     EAQKIDRLVQSFSGAYSQQNPGVVSNADAAYLLAFQTIMLNTDLHNPSIPEKNKMTMD
                     GLKRNLRGGNNGEDFDAKFLEELYSEIKAKPFELNFVKTSPGYELTSITLNKDSTFKK
                     LDSFLHSTDVNINTVFPGIGDNVKTTVDQPKSWLSFFTGYKGTITLTDKQTSAQATIQ
                     VYTPNIFSKWLFGEQPRVIIQPGQTKESIDLAAKIAAGFSSPVKNFKATYDYEAGDLI
                     KAYDNQKKLISTNKNTVIKGAMFRTPNAEQQETSKSATKTLNTDYDINEDTETIKDES
                     KSSYKK"
     gene            3490..>4017
                     /locus_tag="U6309_002734"
     CDS             3490..>4017
                     /locus_tag="U6309_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEN5506147.1"
                     /translation="MTDIAVIINNRAKNAHRMEDYLSKLRAHDIDYQLYKADPQQLEK
                     NIQHCCSKYPLLLIGGGDGTVRSAAQWCSNTSVILGVLPLGTMNHFSKELNLPSTTEE
                     LIAAIVEKTTTTIDVAEVNGHIFINNSSIGFYSRLAKNRDYYTNFYNKWLTYIPSFIQ
                     ALSYHPSYALLIKNKE"
BASE COUNT         1365 a          740 c          679 g         1233 t
ORIGIN      
        1 aatcgagaca tcaagtttag ataaaaacac gtgcaagcct ttaaagtcga atataaaaac
       61 tctccaaaac ctggcagatg agaccactca tttaacaaaa aggcaaactt gacatatcaa
      121 aaatataaag acttttgggg gagaataaag tgaacattta gattgggttc agacccggcc
      181 gataatctaa aactaattca atttatttta tgagactgta gagcagattt ttctttgtct
      241 aatagagtat atatatctag cgcccccgcc aattctgttt ttatccccag gggtaatttt
      301 tgtttttgta aatgtaacaa tacttcatca gcgcttgtgt cattagataa tttatttact
      361 attttcaagg ctttttccag ttcattaatt tctgctttaa ttttcttgat ttcaaaatgc
      421 ttatcaatcc ctctaaaaag agtaaaggaa ctttttttga tcacgtcgtt ttcaatgtat
      481 tttttatgat ccagcctgtc ttttatgcct ttgcctagaa tattcccgac aatttcttta
      541 aaaggtctct ctctgatatt gcccaataaa aattgtatgc gttctctctg agacatttct
      601 aaaaattcag accattgttt ttctgagtta tcaagaatat cttccagatg cttctgctca
      661 ctctcggcca aaaaagctaa aaggtttttc tccaccctct gtctcttgta atcgatggtt
      721 gtattttttg atgatttgga ttgtttacct gatgtatgtc caagcaaatc cctgtattgc
      781 tctgcattta aacctttctt actgactagt cgctgcgcct cttttggtga agcaatcata
      841 aggctatcca gaatagaatc gatttgagtc atgcgataca gctctggaga ttgcctgaat
      901 agtgcgtcag gattaatcag cttcacccca ttaaccactt tgtagttggc aaaattttca
      961 ttaaataaaa atttcttttt tgacaacagc aaaatatgct caatggtcat tcgtgaacaa
     1021 ttttctcggt ccgtttgaat caaacgaaaa tgttttgaat ccttcgcctt tggatctcta
     1081 tcaggctcta accggtaagc atcgcaacca gggaatcggc tcatgatttc atcaagcgcg
     1141 gcatctcccg cactatcatt agccgcatcc aaacaaaaaa catgaagctt cccatcaacg
     1201 ataaaaaaat caaatgctgt ccagtggtgg ttatctgcag taaaaagagc gatttgaaat
     1261 cgggtcgttc cactaaaatc gttaatgtta ttttttaact tatccaggaa ttttcggaac
     1321 tcatcttcct caaacgcatt ttgtggtctg ctcagcacaa aacagttaaa tgcctcacca
     1381 cgtttattcc gattttcttt ttttcttgcc atagctaact taaccacgga agggccccag
     1441 cgcgggttat tcaataactg ctcaagccac ttttgattaa gttgttcttt ttcttctttt
     1501 aacccttttt tgactttgga ttcatcttcc atagccgctt atcttcaatc tgattctata
     1561 ttatctagta tagaacgtat aaagcacact acgatatttt tgtaatcatc aagaacttaa
     1621 gtctgcaaat gtttcttgtc taaaaaggca aaattagatc aattggatta taatataacc
     1681 acaaacgatc gctattaaaa cggaatgatg attaattttt tatctaagca aaattcatca
     1741 gttcattcga caaaaggagc agcttatgca tccagaaatt gaaaaggcac aaagagagat
     1801 tattgaggct tttaacgcca aacctaaaaa tgggataaaa aaaataaaag aaatatgtgg
     1861 ggaatataaa atttccccga atgaagagat tgctgctttc tttcatcaac aaagaaaaaa
     1921 tttagattta gaagcggttg gcgattattt gagtgagcca gaagcagaaa atcagcaagt
     1981 attaaaagct tttaccagtc aaatggattt caatgggcag agttttgttg agggtttaag
     2041 gatctttttg aaaacattta aattaccagg agaagctcaa aaaattgaca ggttagtgca
     2101 aagctttagt ggcgcctatt cccagcaaaa tcctggtgtg gtttccaatg cggatgctgc
     2161 ctatctttta gcatttcaaa ccattatgct taatactgat ttgcataacc caagtatccc
     2221 tgagaaaaat aaaatgacta tggatggact caaaagaaat ctcagaggag gtaataatgg
     2281 tgaagatttt gatgccaaat ttttagagga actatacagt gagattaaag ccaaaccttt
     2341 tgagttgaat ttcgttaaaa ccagcccagg atatgaactt acttcaatca ctctcaataa
     2401 agattctacc tttaaaaagc tggattcatt cttgcattca acggatgtaa acatcaatac
     2461 cgtttttcca ggaataggtg ataatgttaa aacaactgta gatcaaccaa aatcatggtt
     2521 aagttttttt accggatata agggaacaat aacgttaacg gataagcaaa catccgcaca
     2581 agctactatc caggtttaca ctcccaatat attttcaaaa tggctatttg gagaacaacc
     2641 gagagtaata atacaacctg gacagaccaa agagtcaatc gatttggcag caaaaatagc
     2701 agctggtttt tcatcgccag taaaaaattt taaagcgacg tatgattatg aggcaggtga
     2761 tttaataaaa gcatatgaca accagaaaaa actcatttcg accaataaaa atacggtaat
     2821 taagggggct atgttcagaa caccaaacgc tgaacaacaa gaaacttcaa aatctgcaac
     2881 aaaaacgctt aatactgact atgatataaa cgaggataca gagactataa aagatgaaag
     2941 taaatcgagt tataagaaat aataatttat aaaagattag cttaatgtca acttgatttt
     3001 catcctgatt tttatcactg agaagattga aatgcacgca ctacaagcgc agcaaacatg
     3061 aagacaaagt aaacgtttta agagttccgg ccaccaactt agtagcagtt tatgcagtat
     3121 tagttaacaa gggcttgatg ggatatatag ataatatgta atagttcaaa cctaatctga
     3181 caccattgct agaaaaagtg agagctcccg tttttgatga aagtgcaaac cttaaacaaa
     3241 accaagaaaa actgatagta ccctaaaaca agtttaccta tgaataccat aactaacact
     3301 aataatatat tttactaagc aaaataagta tggaagctgt gttttattta actacttgaa
     3361 gtttcaaaat gcgtctcata gacatacaca agttagtaaa ggcaaattaa ccggcaggtg
     3421 cttatgatgc cttactataa tttttgaatg taacctaaac tttaagtaag caggacattg
     3481 ggaagcgtca tgacagacat tgcggttatt atcaataatc gagctaaaaa tgcacaccga
     3541 atggaagatt atttatccaa actcagagcg catgacattg attatcaatt atacaaagca
     3601 gaccctcaac aattggaaaa gaatattcag cattgttgct caaaataccc tttactatta
     3661 atcgggggtg gagacggtac tgttcggagt gctgctcagt ggtgcagcaa tacgtctgtt
     3721 attcttggtg tgcttccttt aggaactatg aaccattttt ccaaagagtt aaatttacct
     3781 tcaactactg aggaattaat agcagcaatc gtggaaaaaa caacaaccac aattgacgta
     3841 gcagaagtaa atggccatat ctttattaac aactcgtcta ttggcttcta ctcaaggctt
     3901 gcaaaaaacc gtgattatta tacaaatttt tataataaat ggcttactta cattcccagt
     3961 tttattcagg ctttgtcata ccacccctct tatgccttat taataaaaaa taaggaa
//
LOCUS       DASLOE010000217         3942 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.218, whole genome shotgun sequence.
ACCESSION   DASLOE010000217 DASLOE010000000
VERSION     DASLOE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.218"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            28..1176
                     /gene="dupB"
                     /locus_tag="U6309_002735"
                     /gene_synonym="laiF"
     CDS             28..1176
                     /gene="dupB"
                     /locus_tag="U6309_002735"
                     /gene_synonym="laiF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector deubiquitinase DupB/LaiF"
                     /protein_id="HEN5506148.1"
                     /translation="MPIILDSDVLEVAEYVYKTRLSQPYTEVGSEWEYNYKNPTATFA
                     KGDGHNLQRYITIDGKQLHRPIHGLAHTMRTLMYSQLMYCSSKKQPSPHVCQDGRTIA
                     DLSELDLKKINIAQLFFVAGRESEASYGDAYHRYHLYGAKQFEEYARKHLTHLFSEEE
                     IRLYSRCIEDRVGDSFDGTPEGYIIHLSHMIDLMRCKSPVEVFLGHSKGVSGIVPTLI
                     HLFGKQDGLDIMHYARGLFAATGEAVPYIDSSEWPHLGVDLSRVQRALSIVGDINVPG
                     QEADSKKTAQAGFSVDGCYSALTSVPTPSWYEKELKEIDDEKIDVKEVDDREIEKEHE
                     IVVPSQATTPLSTVKTDSFVEKLLKPFMIWKKPEVQTTQPTTEKTNKP"
     gene            1247..3670
                     /gene="sdjA"
                     /locus_tag="U6309_002736"
     CDS             1247..3670
                     /gene="sdjA"
                     /locus_tag="U6309_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdjA"
                     /protein_id="HEN5506149.1"
                     /translation="MFSYLDKLLDGIFGYQKSDTSQLSTSTPPTLTTVPLKQEYFVKI
                     GESETQDLGLLPVVSKRHNQSVPLEEIPFEDTRLELIELYIALLKQCVLDEKLKSIPA
                     QYLISHYLFIKTLAANEGNKGRKDLYLNLSQKVADYLEKNESKIWSMAVECAKTSEYP
                     IVDWIKKHHLHFNFIRAFILDYNKKSLTHNQRAFMQQFRDSAAFFFPDQVYLAWLTQS
                     YEPGSILNPMYRESRSTHYYHANITDNLLLRTRPKQVNFGPQHFFQKGKGPVKNTYRF
                     NINDGKLMRIQGRTLLFSTNKGNEVIAVKVQKKGEPQSALSNEFQMADYLLKHQRRLN
                     LQSQLPTPLSQYSINRTEILEKCSKSPDFEKFKNLISDAKSLEIYVYKATPSYFTYLH
                     DKQQSFSQLTSSVQKNVHDLFVLLREGIVFPYLADMFHTHIDESKRSDKGRYQTLVEL
                     LSALQSQMGRLDKWQKAVEFVNLRASGIADLGDNLPLTSFLTVSDWTKHYHAELLTGV
                     YHPSFLFLDKSSGSVRSLFNSRRKIFGNYLYLNIIAEYLLVIQLVIGCYGDKVTRKMN
                     SKSKAKVWEHLAELMFSSCAEAVNLITGMPKSRALAFLKQRANVRKHTQQTSFWMTPD
                     YSNLDRLSVQSQQSTLYPGESDYEINSDLISGVGLSLDGINQDLGDYNQASPLRELEK
                     LLYATVTLIEGTQQLDKQFFQQLDETEKMILSAAGVDKCYQAVAKLLDLARPGCQMQR
                     RLAFTYYEMIKRIYPCSNPADVRFELVAKEEAIIKIQRFWREHKKENQSLEKGFDFDR
                     NTSSSQSPL"
BASE COUNT         1241 a          706 c          818 g         1177 t
ORIGIN      
        1 tcattttgtt tatggaagga gaccattgtg cctattattc tagattctga tgtgttggaa
       61 gtggcggaat atgtgtataa aacacgctta tctcagcctt acactgaagt gggttctgaa
      121 tgggagtata attacaagaa tccaacagcc acttttgcaa aaggcgatgg ccataacttg
      181 caaaggtata ttacaattga tggcaagcag cttcatcgcc ctatccatgg gttagctcat
      241 acgatgcgca cattgatgta tagccaattg atgtattgct cctcaaaaaa acaaccttcc
      301 ccacacgtat gtcaagacgg acgaacaata gccgatctca gtgaacttga tcttaaaaag
      361 ataaacattg cgcagctatt ttttgtagcc gggcgcgaaa gcgaggcttc ttatggtgat
      421 gcttatcatc gttatcatct gtatggggct aaacagttcg aagaatatgc ccgcaagcat
      481 ttgactcatc ttttttcaga agaagaaatc cgattgtact cacgatgtat agaagacagg
      541 gtaggtgaca gttttgatgg tacccctgaa ggttatataa ttcacttgtc acacatgatc
      601 gatttgatgc gttgcaaaag cccggttgaa gtatttttgg gccactcaaa aggggtttct
      661 ggtattgtac ctacattgat tcacttgttt ggcaaacaag atggcttgga tattatgcat
      721 tacgcccgag gcttgtttgc ggctacaggt gaggcggtgc cctatattga ttcatctgaa
      781 tggcctcatt tgggagttga cttgagtcgg gtacaacgcg cactctctat cgttggtgat
      841 atcaatgtcc ctgggcaaga ggctgattct aaaaaaacag cgcaagccgg tttttctgtg
      901 gatggttgtt attctgcgtt aaccagtgtg cctacacctt cctggtatga gaaagagtta
      961 aaagaaatcg atgatgaaaa aatcgatgtt aaggaagttg atgataggga gatagaaaaa
     1021 gaacatgaga tagttgttcc ttcgcaggca acaacacctt taagtacagt aaaaactgac
     1081 tcctttgtcg aaaaacttct gaagccattt atgatttgga aaaagccaga ggtacaaacg
     1141 actcaaccaa caacggagaa gactaataaa ccttagcctg ttttatttaa agtaatagag
     1201 aaacggattc acttagggaa ttaaaacgca atgaggtgtt gtaactatgt ttagttatct
     1261 ggataaatta ttagatggaa tatttggata ccaaaagtca gatactagtc agttaagcac
     1321 ctcaacgcca cctactctaa ctactgttcc tttgaaacaa gaatattttg taaaaattgg
     1381 ggaaagtgaa acacaggatc taggcttact gccagtggtt agcaagcgac ataatcaatc
     1441 agttcccttg gaggaaattc cttttgagga taccagactt gagttgattg agctttatat
     1501 tgcattgtta aaacaatgtg ttttagatga aaaactgaag agtatccctg cgcagtatct
     1561 tatatctcat tatcttttta taaaaacctt agccgcaaac gagggaaata aaggtcgaaa
     1621 agatctgtat cttaatttat ctcaaaaagt ggctgattat cttgaaaaaa atgaatctaa
     1681 aatatggagt atggctgttg aatgcgctaa aaccagtgag tatcccatag ttgactggat
     1741 aaaaaagcac catcttcatt ttaattttat aagagctttt atactggatt acaataaaaa
     1801 atcattgact cataatcaac gtgcgtttat gcagcagttt agggattctg ctgctttttt
     1861 ctttcctgat caggtttacc ttgcttggct tacccaatcc tatgaaccag gctctatctt
     1921 gaatcctatg tacagggaga gcagatcaac gcattattat cacgcgaaca ttactgataa
     1981 tctcttgtta agaacacgcc ccaaacaggt caattttggt cctcaacatt ttttccaaaa
     2041 aggaaaaggc cccgtcaaga atacttatcg tttcaatatc aatgacggaa aattaatgcg
     2101 catacaggga agaacattac tttttagcac gaataaaggc aatgaagtga ttgctgtaaa
     2161 ggtgcaaaaa aaaggggaac cgcaatctgc tttaagtaat gaatttcaaa tggcagatta
     2221 tttgttgaaa catcaacgcc gtttgaattt gcaaagtcaa ttacctaccc ctctcagcca
     2281 atattcaatt aatcgaacag agattctcga aaaatgcagt aaatcacctg attttgagaa
     2341 gttcaagaat ttgataagtg atgcaaaaag ccttgaaatt tatgtttata aagcaacacc
     2401 ttcttatttt acttatctgc atgataagca gcagagcttt agtcaactca cttcatcagt
     2461 gcagaaaaat gtacatgatc ttttcgtgct cctgagagag ggcatagtct ttccttatct
     2521 ggcagatatg tttcacaccc atattgatga gagtaaaagg agcgataagg gtcgatatca
     2581 gacgttagtt gagcttttaa gtgctttgca atcacaaatg ggccgtcttg ataaatggca
     2641 aaaagcagtc gaatttgtta acttacgagc cagcggtatc gctgatttgg gtgacaatct
     2701 tccgttgacc agcttcttaa ctgtttctga ctggacgaag cattatcatg cggaattgct
     2761 aacgggcgtt taccatccat cctttctatt cctcgataaa tccagtggct cggttcgctc
     2821 cttgtttaac agccggcgca aaatattcgg caattatttg tatctgaata tcatcgctga
     2881 gtatctatta gttattcaat tggtaattgg ctgttatggc gataaagtga cccgtaaaat
     2941 gaatagtaaa agcaaagcta aggtttggga gcatttagcc gaattgatgt tttccagttg
     3001 tgctgaagcg gttaatttga taactggtat gcccaaatcc agggcacttg cttttttaaa
     3061 gcaaagagcc aatgtaagaa aacatactca acaaacgtcg ttttggatga ctcctgatta
     3121 ttcgaatctg gataggctga gtgtgcagtc tcaacaatcc actttgtacc ccggcgaatc
     3181 agattatgaa ataaacagtg atttaatttc gggggtcggt ctgagtcttg atggcatcaa
     3241 tcaggatttg ggtgattata accaggcaag ccctttaagg gagctagaga aattattgta
     3301 tgctacagtg accttaatag agggaaccca gcaactggat aagcaatttt ttcaacagtt
     3361 ggatgaaact gagaaaatga ttttgagtgc tgctggagtt gataaatgct atcaagctgt
     3421 cgcaaaactc ctggatcttg ctcggcctgg ttgtcaaatg cagcgaagat tggcttttac
     3481 ttattatgaa atgatcaaaa gaatatatcc ctgctcaaat cccgcagatg tgagatttga
     3541 acttgttgca aaagaagagg cgattataaa aattcagcgt ttttggcgag agcataaaaa
     3601 agaaaaccaa tcgcttgaaa aagggtttga ttttgacagg aacactagtt catcgcagtc
     3661 gcctttatga aagggtcatt tcaattgtaa gagatcatcc ccaagatcgt tagaggatat
     3721 ttcaacatca gggcactgac ctgttacttt taattttgga aaattaaaag tactcataag
     3781 cttaatgaga tttttcttat ttccgctaac aattggattt tatgctattt aattaagaca
     3841 agattttatt cttcatccag ggaattatgg tggaagttta aactcaatat cagatatgat
     3901 tacgctttga attgacaaaa acaagacaaa aaacgatact tt
//
LOCUS       DASLOE010000218         3925 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.219, whole genome shotgun sequence.
ACCESSION   DASLOE010000218 DASLOE010000000
VERSION     DASLOE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.219"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            92..2110
                     /gene="legC6"
                     /locus_tag="U6309_002737"
     CDS             92..2110
                     /gene="legC6"
                     /locus_tag="U6309_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegC6"
                     /protein_id="HEN5506150.1"
                     /translation="MSHSKREQNPIARNLSDFRQVATLLNNEGLVVTEHNYKKVQALL
                     NRYKELATFNDQNQSKLNGKQIGELNQIRLIISPEERREIRKSVTRIENGIKNREKRI
                     KEEYTRIEKQQAKAREAVQVLWPQLQEAAKKFINSGIANMEELLKEYKDLRNELYNHA
                     LNLPTQERKQKNQEMAELSRLVNIHESKLYEEINRARKVIEAAVTQAQKNVDSMSLLQ
                     MVRLMLPKLKDADILKIAQKAGVLSLQDTTTSEHGANLDKLVVRDQPLEKQSALISIM
                     FKELALNGAQAGPLAKYLQAVQENAKDLISGKKTHFTLLYQTNEGEIGGFYFNIHELL
                     QNQHQAISKLVHEMYKRGVDQNSRVVMAFASPEQAASLMLANGENIKALTEAGNLQEL
                     TEQLLENAGDSKALARIVGHDVVIGQKTSEIIRDLNRSKEQNGIEPAEVMKLFQIIAV
                     IFETRNILINWITAPVHLKGQTEEALLGAGKTPAIVEGKGTQPKRVRFLPDLVEKPKA
                     TLPDTEQPVTKRKDDELLDKKIQVALEKVDQALEELKGKIGDINQHKYDKAYEAAKVL
                     LRQLQETRDQYAIDLANPQINFKEAGLKFKEASNVVIQQAKPILEKDLGWGEYLKNLF
                     KVIVNAIVFCVTFGASQGFFATTRAKSAEAVEKAESELELNQVGSLPK"
     gene            complement(2218..2775)
                     /locus_tag="U6309_002738"
     CDS             complement(2218..2775)
                     /locus_tag="U6309_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN5506151.1"
                     /translation="MARTLSNMLPLGTVAPHFELLDVVSGSKVKLRETPRYVATVIMF
                     ICNHCPFVKHINNELTKLANDYIPQGIRFLAINSNDVESYPDDSPENMIQTAKVHQYP
                     FPYLFDETQEVAKAYNAACTPDFFVFDSDLSLVYRGQLDDSRPGNGIPVTGNSIRQAL
                     DCLLNNKPFDFEQKPSIGCNIKWKI"
     gene            complement(2799..3299)
                     /locus_tag="U6309_002739"
     CDS             complement(2799..3299)
                     /locus_tag="U6309_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506152.1"
                     /translation="MSKTKYDTIYLVLGIVGALLLIYGLSQNNPQVYYITGSLALMIT
                     AIHYKLIYFIALELILGAGHTSLLLGMGRYIGLALPSLLCLQLLIFYLMFGKGNSIFL
                     LTGIIGIALLSMGLAYHNQIIFFSGSSGVAAYSYYSAYRGQYICYIWGILNTIFASYA
                     LYQLVF"
     gene            complement(3303..3791)
                     /locus_tag="U6309_002740"
     CDS             complement(3303..3791)
                     /locus_tag="U6309_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced entry protein EnhB"
                     /protein_id="HEN5506153.1"
                     /translation="MSLINYFTKTLLITAFATNAVQAASIFPRGCEVTGFGYSENYLI
                     LNETGNQTYYLIQNRSDSKIELERFDNSDAFMIPPLQASLDPMSWAAFASDVKNLNFK
                     CYKHVDENTALVDCRDVLEVCQYPRVKFALSNMGNYWISTNKSQNEVIQESVAKGIYL
                     KW"
BASE COUNT         1462 a          653 c          717 g         1093 t
ORIGIN      
        1 tgtaattttg taaaaaattg cccaattaaa tgtgacaaaa tagcgttcgt tgattataat
       61 gcgagcacac taaccatcaa ggaatcattc tatgtctcat tccaaaagag aacaaaatcc
      121 tattgcccgg aatttaagtg actttaggca agttgcgaca ctattaaata acgagggtct
      181 tgttgttact gaacataact ataaaaaagt ccaagcctta ttaaatagat ataaagagct
      241 agcaaccttc aatgatcaaa accaaagcaa attaaatgga aaacaaatag gtgaactaaa
      301 tcaaattaga ttaattattt ctccggaaga gcggcgagaa atcagaaaaa gtgttacacg
      361 tatcgaaaat ggaataaaaa atagagaaaa aagaataaaa gaagaatata cgagaataga
      421 aaaacaacag gctaaggcaa gagaagcagt tcaagtttta tggccacagt tgcaagaagc
      481 agccaaaaaa tttataaatt ctggaatagc aaacatggaa gagctattga aagaatataa
      541 agacttaaga aacgagttat ataatcatgc gcttaatctt cctactcagg aaagaaagca
      601 aaaaaatcaa gagatggccg agttatctcg tcttgttaat atacatgaaa gcaaattata
      661 cgaagaaatt aatcgagcca ggaaggtaat tgaagctgca gtgactcaag cacaaaagaa
      721 tgtagacagt atgtcattgc tgcaaatggt tcgactcatg ttgcctaaat tgaaagacgc
      781 agatatatta aaaattgctc aaaaagctgg agtactttcc ttacaggata caacaacctc
      841 tgaacatggg gcaaatttag ataaattggt cgtaagagac cagccattag aaaaacagtc
      901 tgctctaatt tctataatgt tcaaggaatt ggctctcaat ggtgctcagg ctggtcctct
      961 ggcaaaatac ctccaggcag ttcaagaaaa tgcaaaagat ctaataagtg gtaaaaaaac
     1021 tcacttcact ctgctttatc aaacaaatga gggtgaaatt ggagggtttt attttaacat
     1081 tcatgaattg ttgcagaatc agcatcaagc aatttccaag cttgtacatg aaatgtataa
     1141 gagaggagta gatcaaaaca gtcgagttgt aatggctttt gctagtccag aacaagctgc
     1201 gtctttgatg ttagctaatg gagaaaatat taaagcatta actgaagccg gcaatttaca
     1261 agagttaact gaacaattat tagaaaatgc tggtgattca aaagcattgg ctcgcatagt
     1321 aggacatgat gttgtaattg gccagaaaac ttcagaaata attagagatc ttaatcgttc
     1381 aaaagagcaa aatggtatag agccagctga agtaatgaaa ttatttcaaa taattgcagt
     1441 aatatttgaa actcgaaaca ttttaatcaa ttggataact gctcctgtgc atttgaaggg
     1501 gcaaacggag gaagctttat tgggcgcagg taaaactcct gccatcgttg agggcaaagg
     1561 aacccaaccc aaacgcgtaa gattcctacc tgatttagtg gaaaagccaa aagctacatt
     1621 gccagatact gaacaacccg ttaccaaaag aaaagatgat gaactacttg ataagaaaat
     1681 acaagttgca ttagaaaagg ttgatcaagc attagaagaa ctgaaaggta aaatagggga
     1741 tatcaatcaa cataaatatg acaaagccta tgaagcagca aaagttcttc taagacaatt
     1801 gcaagaaaca cgtgatcaat atgctattga tttagctaat cctcaaatca atttcaaaga
     1861 ggcagggctg aaatttaaag aagctagtaa cgtggtgatt caacaggcca aacctatcct
     1921 ggaaaaggat ttaggttggg gagaatacct caagaacttg tttaaagtga tagtaaacgc
     1981 tattgtattt tgtgtgactt ttggagcaag tcaaggcttc tttgctacta cacgtgcgaa
     2041 atctgctgaa gcagtggaga aagcggagag cgagctggag ctaaatcaag tcggttcatt
     2101 accaaaataa tacatatggt taactagttt gttatgagag caacatacga tttttattat
     2161 acatataatg agtaatggaa atcgatgttg cttttttgaa ttaaagatag cttaagccta
     2221 tattttccac ttgatgttgc atccaatact tggtttttgt tcaaaatcaa aaggtttatt
     2281 atttaataag caatccaaag cctggcgtat tgagttgcca gttacaggaa taccatttcc
     2341 tggtcttgaa tcatctaatt gccctcgata aactaaagac aaatcagaat caaaaacaaa
     2401 gaaatccggt gtgcaagctg cattatatgc ttttgctact tcctgagttt catcaaaaag
     2461 atatggaaat ggatactgat gaactttagc agtttgaatc atattttcag gtgagtcatc
     2521 agggtaactc tctacatcat tggagttaat agctaaaaaa cgaattcctt ggggaatata
     2581 gtcattagca agttttgtta attcattatt gatatgtttc acaaaggggc aatggttgca
     2641 tataaacata atcacagtag ccacataacg tggagtctct cttaatttaa ctttacttcc
     2701 actaaccaca tccaataatt caaaatgagg agctactgta cctaatggaa gcatgtttga
     2761 tagagttcta gccattttat taaatccctc aaataatctt aaaataccaa ttgataaaga
     2821 gcatatgacg caaatattgt attaagaatg ccccatatgt aacagatgta ttgacctcta
     2881 tacgcactat aataagaata tgcagctacg cctgaacttc cagaaaaaaa tatgatttga
     2941 ttatggtaag caagccccat cgaaagcagt gctattccta taatgccggt taataaaaaa
     3001 atactgtttc ccttgccaaa cattaaataa aaaatcagta gctgtaaaca cagcaaagaa
     3061 ggtaatgcta atccaatata tctccccata ccaagaagta gggaggtatg gcctgccccc
     3121 aaaattaatt ccagagcaat aaaataaatc aatttatagt gaattgctgt aatcatgaga
     3181 gcaagtgagc cagtaatgta atagacttgg ggattattct ggctcagccc ataaataagt
     3241 aacaaagcac caacaatacc tagtaccaga tatatcgtgt catactttgt tttactcacg
     3301 gatcaccatt ttaggtaaat tcctttagca accgattcct gtataacttc attttgtgat
     3361 ttattagtag aaatccaata gtttcccata ttactaagtg caaatttaac acgaggatat
     3421 tggcagactt cgagaacatc tcgacaatca actaaagcag tattttcatc aacatgctta
     3481 taacacttaa agtttaaatt ttttacatct gaggcgaatg ctgcccaact cattggatct
     3541 agcgatgctt gtaagggagg aatcataaac gcatctgagt tatcaaaacg ttcaagctca
     3601 attttcgaat ctgaacggtt ttgaatgaga taataggttt gattacctgt ttcatttaaa
     3661 attaaataat tttcactata accaaaacca gtgacttcac agccacgggg aaaaatcgaa
     3721 gcagcctgga ctgcattggt cgcaaaagct gtgattaaca gcgttttagt aaaataatta
     3781 attaatgaca taatttcctc agaaaacaaa attaagatgc tattttaatc attaaataga
     3841 caaagtctat cacggaataa aaaaatcaaa cgcatagaag ctattaattc gtctttatta
     3901 tatctttgtt atcaggtatt cataa
//
LOCUS       DASLOE010000219         3890 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.220, whole genome shotgun sequence.
ACCESSION   DASLOE010000219 DASLOE010000000
VERSION     DASLOE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.220"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            83..2080
                     /locus_tag="U6309_002741"
     CDS             83..2080
                     /locus_tag="U6309_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEN5506154.1"
                     /translation="MLNSQQMAAVKYIDGPLLVLAGAGSGKTRVITQKIGYLINSCGY
                     NANSICAVTFTNKAANEMRARVSAFLPAVSRRGLKVATFHTLGLSIIKRDVSRCDLKP
                     GFSIFDSEDCLQILRGFLPVNKATDRDFILKVQQQISLWKNNLLNPELILQNPPETLL
                     CDEALTIYPRYQEALKVYNAVDFDDLIRLPVDLLNDHADVLEYWQNKIRHLLVDEYQD
                     SNTSQYLLVKKLAGVRAHFTVVGDDDQSIYAWRGAKPENLAQLQKDFPRLKIIKLEQN
                     YRSTSRILHAANHLIAHNQHLFEKKLWSDLGHGELLRVVSCKDEQDEAEHVIADLISH
                     KLRNRTNYSDYAILYRGNHQARIFEKVLRHHGIPYHISGGQSWFAKLEVRDIFAYLKL
                     LCNEADDAAFLRAITTPKRGIGESSLDALGRYAQSRGISLYHGSDHLALSEFVADKQR
                     MIFHDFKLWMEDIKKRISSDSVLEHLRQMVEDIGYEAYIYEQCDSPAKAQKKMDNVWE
                     LLEWVNRLLNKEPEQSLMDVVNKLILIDILEQSDESSSDVVQLMTLHASKGLEFPFVY
                     LVGMEEELLPHRVSIDDDQIEEERRLAYVGITRAQKGLCFTLAKQRRRAGELQDCVPS
                     RFLDELPQESLEWFGKGGERCEEQSKKLAKSHLEGLKNLLS"
     gene            complement(2164..>3890)
                     /gene="iroT"
                     /locus_tag="U6309_002742"
                     /gene_synonym="mavN"
     CDS             complement(2164..>3890)
                     /gene="iroT"
                     /locus_tag="U6309_002742"
                     /gene_synonym="mavN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T4SS effector ferrous iron transporter
                     IroT/MavN"
                     /protein_id="HEN5506155.1"
                     /translation="EYLLNHFPNTDEENCPQFFKDYKKQLTLLAEFGHKELNQDSKKR
                     KRQIEKTLSDMEKWFALQLFSDKNNPSPNTSKYTIELLDWLALNKRDEWIKQYEKRHF
                     QFHIVKGFSVVAGLFMGLGSTYLIVEAFTIIPFFATIPFTLWPIIILPMATVAGAAYG
                     MLTYNAVTDMINNNTVVKWYNKIRNDLSQGLTVRNVFIATTAVFLVGLALALTVCTAG
                     TWWTIATNARPLFDWMKKMPSFIMGIINPIITGASAIVFNIQNTAESLDMVDEATRSN
                     KNVFQKIYEAISNGYQHLRETENWLQIVNPFRILLKLTITPLRILLFLGHLISVAVTS
                     DRMPGVPQILSALVAIISEGFEDAHYFIGHTHEDELDEEHGHQDHHQFEKLLKERLDP
                     DSDQDHNMDIPTWILKTIASPIYGLAALWDCSASKLNPSQENHSKNLPSGKKPHVLSL
                     GEAWNKQWGVAKEFNVEFSTHAKRPSQEWQVEHAVAQIDKFQRKHLKDIVIGRELADK
                     KIIALNHLKEKIRHPEKGETLSDTLEQAKKQPDYNQHRLFNQKGEKTRTQLFIEELPE
                     RINLSSGK"
BASE COUNT         1141 a          667 c          859 g         1223 t
ORIGIN      
        1 gatgtagaac gctctatcaa ttaattcttg cgtgaaaaag aaagcctggg tgataatgcc
       61 ttttttttga gcgggaataa gtatgttgaa tagccaacaa atggctgcag taaaatatat
      121 tgatgggcct ttgttggtgc ttgccggggc tgggagtggt aaaaccagag ttattacaca
      181 aaaaattggt tatttaatta attcatgtgg ttataatgcc aactccatct gcgccgttac
      241 tttcacgaat aaagcggcga atgaaatgcg cgcaagggtt tcggcctttt taccagctgt
      301 aagcaggcgg ggattaaagg ttgcgacatt tcatacatta ggcctaagca tcatcaagcg
      361 tgatgtttct agatgcgatt tgaaaccagg attttccatt tttgatagtg aagattgttt
      421 gcaaattttg cggggttttt tacctgttaa taaggcaacc gatagagatt ttatcctcaa
      481 agttcaacaa caaatttctc tttggaaaaa taatttattg aatcccgaat taatactcca
      541 aaaccctccg gaaacacttt tatgtgacga ggcgttaaca atttatccac gctatcaaga
      601 agcattaaaa gtatataatg cagtggactt tgatgattta attcggttac ctgtcgatct
      661 actgaatgat catgctgatg tgttggaata ttggcagaat aaaattcgtc atttgttggt
      721 ggatgaatat caggattcaa acactagcca gtatcttctg gtaaaaaaat tggcaggggt
      781 tcgtgcccac tttacggttg tgggtgatga tgatcaatcg atttatgcct ggcgtggtgc
      841 caaaccagag aatttggcgc agttacaaaa agattttcct cggttaaaga ttattaaatt
      901 agaacagaat tacagatcaa ccagccgaat tttacatgct gccaaccatc tgatcgctca
      961 taatcaacat ttatttgaaa aaaaattatg gagtgatttg gggcatgggg aattattgag
     1021 ggttgtcagc tgtaaggatg agcaggatga ggcggaacat gtgatcgctg atttaataag
     1081 ccataaatta cgtaatcgga caaattatag tgattacgct atcttataca gaggaaatca
     1141 tcaagcaaga atatttgaaa aggtattaag acatcacggt attccttatc atatcagcgg
     1201 aggccagtcc tggtttgcca agttagaagt cagggacatt tttgcctatc ttaaattact
     1261 ttgtaatgaa gcagatgatg ccgctttttt gcgagcaatt actaccccca aacgaggtat
     1321 aggagagagc agtctcgatg ccttgggacg ctatgctcag tccagaggga taagtttata
     1381 tcacggatcc gatcatttgg ccttgtctga gtttgttgct gacaagcaga gaatgatttt
     1441 tcatgatttc aaattgtgga tggaagacat taaaaaacgt atcagttcag attcagttct
     1501 ggagcattta aggcaaatgg tggaagatat tgggtatgaa gcttatattt atgaacaatg
     1561 tgattctccc gccaaggcac aaaaaaaaat ggataatgta tgggagcttt tagaatgggt
     1621 taatcgttta ttgaataagg aacctgaaca atcgctcatg gatgttgtga ataaattaat
     1681 actaatcgat atactggaac aatctgatga atcttcaagt gatgtagtcc aattaatgac
     1741 attgcatgcc tcaaaaggtt tggaatttcc ttttgtttat ttggtcggga tggaagaaga
     1801 attattaccc caccgagtga gtatcgatga tgaccaaatt gaagaagaaa gaagattggc
     1861 ttatgtaggg atcacacgtg cccagaaggg attatgtttt actttagcga agcagagacg
     1921 tcgagctgga gaactgcaag actgtgttcc aagccgattt ttggatgaat tgccgcaaga
     1981 gagtctggaa tggttcggta aaggcgggga gcgttgtgaa gaacaatcaa aaaaactggc
     2041 caaatcccac ctggaaggac tgaaaaactt gttgagttaa gagtacatta tattttatct
     2101 ccagtaagga gtatcattaa ctgaactcct tactgctttt taaggtacta atgttaatct
     2161 ctatcatttg ccagaagaga gattgattcg ttctgggagt tcttcaatga aaagttgtgt
     2221 acgggttttt tccccttttt ggttaaatag tctgtgttga ttgtagtctg gttgcttttt
     2281 agcttgctcc agagtgtcac tcaatgtttc tcctttttca ggatggcgta ttttttcttt
     2341 taaatgattg agggcgatga tttttttatc ggctaattcg cgaccgataa cgatgtcttt
     2401 gagatgcttt ctttgaaatt tgtcgatttg ggcaacagca tgttccactt gccattcttg
     2461 cgatgggcgt ttggcatgag tactaaattc aacattgaac tctttggcta ctccccattg
     2521 tttattccat gcttctccaa gacttaacac atgaggtttt ttgccagaag gcaaattctt
     2581 actatgattc tcttgagaag gatttagctt actggcagaa caatcccaga gtgctgccaa
     2641 gccatatata ggtgaagcaa tggtttttaa tatccaggta ggaatatcca tattatgatc
     2701 ttgatcggag tcaggatcta gtctttcctt taggagtttt tcaaattggt gatgatcttg
     2761 gtgtccatgt tcttcatcca gttcgtcttc atgggtatgt ccaataaaat agtgggcatc
     2821 ttcaaagcct tcgctaataa tggcaacgag cgcggaaaga atctgcggta ctccaggcat
     2881 acgatccgat gtgactgcaa cgctgatcaa atgccccaga aacaaaagaa tacgcaatgg
     2941 ggtaatggtt aattttaaaa gaatgcgaaa cgggttaact atttgtaacc aattttcagt
     3001 ttcacgtaag tgctgatatc cattgcttat tgcttcgtaa attttttgga agacattctt
     3061 attactacga gttgcttcat cgaccatatc cagtgattca gccgtgtttt gtatgttaaa
     3121 gactatagca gaggcgccgg ttataatggg attgataatt cccataataa aactgggcat
     3181 ttttttcatc cagtcaaata aagggcgggc atttgtagca atcgtccacc atgttccagc
     3241 agtacagacg gttagggcta gtgccagtcc aacaagaaaa acagcagtag ttgctataaa
     3301 cacattgcgt acagttaaac cttggcttaa atcattgcga attttattat accatttgac
     3361 aactgtgtta ttgttgatca tgtcggtaac agcattatag gtcagcattc cataagcagc
     3421 ccctgcgacg gtggccatgg gaagaattat aatgggccaa agtgtgaatg gaatggtggc
     3481 aaaaaacggg atgatcgtaa aggcttcgac aatcagatag gtacttccca gccccatgaa
     3541 aaggccggca accacgctaa aacctttgac aatatgaaat tgaaaatgac gcttttcata
     3601 ttgttttatc cattcgtctc gcttatttaa agcaagccag tcaagtagct ctatggtgta
     3661 tttggatgtg tttggagaag gattgttttt atctgaaaac aattgcaggg caaaccactt
     3721 ttccatgtcg gagagtgttt tttcaatttg acgttttctt ttcttactgt cttgattgag
     3781 ttctttatgc ccaaattcag ccaataatgt taactgtttt ttataatctt tgaaaaattg
     3841 cgggcaattt tcttcgtcgg tgtttggaaa atgattcagc aaatactctt
//
LOCUS       DASLOE010000220         3874 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.221, whole genome shotgun sequence.
ACCESSION   DASLOE010000220 DASLOE010000000
VERSION     DASLOE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.221"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1091)
                     /locus_tag="U6309_002743"
     CDS             complement(<1..1091)
                     /locus_tag="U6309_002743"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN5506156.1"
                     /translation="MTVNVNSMKDAVLVAIDIAKARNDVLVQLPNSTKKKFKVANKMK
                     DYKEFIDYLSSFNKPCFIAFEATSNYHRPIAYHLQKAGFTVHLVSSFAAAKTREALHN
                     SWDKNDPKDAQVILHLLKTGVVQTYYDPLINQYNDIQEIAKTYHQISLRKVRVQHSIM
                     NHYLPLYFPEAEMFFHSSRALWFSHFISHFPTPSTILKYTQEEFINAAWDVVGRKVDK
                     RNWLINFYQTAKESIGLPTEPDAQAIRMFCIILQEHQDLCKQLKILEETAESFLEGNN
                     DYKHLLSIPGIGPIIALTILAEAGNLKRFKHYRQFLKFCGFDLSTQQSGFFRGQTKLS
                     KFGNARLRYVFWLAATVAIRMRENTFRKK"
     gene            1243..1659
                     /locus_tag="U6309_002744"
     CDS             1243..1659
                     /locus_tag="U6309_002744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506157.1"
                     /translation="MTRSRPYHSDDNAHVEQKNWTHVRQLFGYERFDKKEVVELMNDL
                     YRNEISLMNNYFLPNTKLIEKQRVDAKIIKKHDKAATPYQRLMKSKHISEEKKQELQK
                     IYNTLNPFELRNNIKKKVDRIFRLINNKGDKNICYT"
     gene            complement(1933..2232)
                     /locus_tag="U6309_002745"
     CDS             complement(1933..2232)
                     /locus_tag="U6309_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506158.1"
                     /translation="MACFPGSSGSPVLICLQGFHDKVGNYHISANKNILLGLLSSGPY
                     LTQSGQIEIKEIPTTNMVPVASFNQMIHLGYYVKAKEILVLYEHIKNQVLLNGNN"
     gene            complement(2281..2793)
                     /locus_tag="U6309_002746"
                     /pseudo
     CDS             complement(2281..2793)
                     /locus_tag="U6309_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2850..3638)
                     /locus_tag="U6309_002747"
     CDS             complement(2850..3638)
                     /locus_tag="U6309_002747"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506159.1"
                     /translation="MLIFNCTKAASDFFTTIRKGNKLSPMSPTPKCELSEEPVLHDHQ
                     QWHWMVHVKKFGHRNVILAMDTDSRFCMIFWGLKKGNIQKFLEQFHDRFSLHIISVIN
                     MGGQDETLLNASMESFLENHHEYAFVPRGDRSVQAHINDALMNLQYEQYRWEDDLPTE
                     EELFISDLRHNDTPRKRKQDRDYIFPTEILYNTWLSRYANLDEQTIKHTISKYRQTNS
                     QLWRFPFDIDVGDLNFNEIETVIAEALSDNVLSFEDYFKQKNCK"
BASE COUNT         1254 a          654 c          669 g         1297 t
ORIGIN      
        1 tatttttttc gaaatgtatt ttcgcgcatt ctaatagcaa cagtggctgc aagccaaaaa
       61 acgtagcgta agcgtgcatt tccgaattta gataatttag tttgacctct aaaaaaacca
      121 gattgctgtg ttgataaatc aaatccacaa aatttaagaa attgtcgata gtgcttaaat
      181 cgttttaaat ttccagcctc agctaaaatc gttaatgcaa taataggtcc aataccaggt
      241 atgcttaaaa ggtgtttata gtcgttatta ccctctaaaa aggattcagc agtttcctcc
      301 agtattttta gctgtttaca taggtcttga tgttcttgca gaataatgca aaacatcctg
      361 atggcttgag catcaggttc ggtgggtaaa ccgatacttt ctttagccgt ttgataaaaa
      421 tttattaacc aatttctttt atcaaccttt cttccaacaa catcccatgc agcattaata
      481 aactcttctt gtgtgtattt caatattgta gatggtgtag ggaaatgaga tataaaatgc
      541 gagaaccaca atgccctaga gctatggaaa aacatctccg cctcaggaaa ataaaggggt
      601 aaataatgat tcataatgct atgctgaacc ctgactttcc gtaaagaaat ttgatgataa
      661 gttttagcaa tctcttgaat atcgttatat tggttgatca agggatcata atatgtttgc
      721 actacacccg tttttaataa atgcaaaatg acttgagcat ccttaggatc atttttatcc
      781 caagagttat gaagtgcttc ccgtgtcttg gctgccgcaa aggatgaaac tagatgtaca
      841 gtaaaccctg ccttctgaag gtggtatgcg ataggacggt gataattaga tgttgcttca
      901 aaagcaataa agcatggctt attaaaggaa gacaaataat caataaattc tttgtaatct
      961 ttcatcttat tagccacttt aaactttttc tttgtactat tcggaagctg aactaaaaca
     1021 tcattacgtg cttttgcaat atcaatagca actagcaccg catccttcat actgttaaca
     1081 ttaactgtca tagttagatt ctcctttagt tgatttcact ggttaactaa aaggattgtt
     1141 tcttaactat gactccattt caaaaggtac tatggaaatg agtttttaaa ctggcatttg
     1201 atccgatatt tcactaatgc agaacgccct agatatattc agttgacacg ttccaggcca
     1261 taccatagtg atgacaacgc ccacgtggaa caaaaaaact ggactcatgt gagacagcta
     1321 tttggatatg aacgatttga taaaaaagaa gtggttgagt taatgaatga tctttataga
     1381 aatgaaatct cattaatgaa taattatttt cttcctaata caaaactcat tgaaaaacag
     1441 agagttgacg cgaaaatcat aaaaaaacat gataaggcag ctacacccta tcaacgatta
     1501 atgaaatcaa aacatatttc tgaagagaaa aagcaagagc ttcagaaaat ctataacacg
     1561 cttaatcctt ttgaattacg aaataatata aaaaagaaag tggatcgtat ttttagacta
     1621 ataaataaca aaggtgataa aaatatatgc tatacataag gtacggaaca gccggggacg
     1681 gtgatgaccg tgaggacatg atcgaagggg gcctcattgc cggccccttc accccgctag
     1741 gatatttata gaggatatta tgttaataac gtctccgttc ggtaacatca attatgaggc
     1801 aacgaatcac cctttcggta acatttattt atgaggcaac aggaggttag gtgtattttt
     1861 acgatggcaa aaaccaaaga tacgtccctt atgcttcaag atttttctgg ttgtttgata
     1921 tagagttgtt aatcagttgt ttccatttaa taatacttgg tttttaatat gctcatacag
     1981 caccaaaatt tctttggctt ttacataata gcctaaatgt atcatctgat taaaagatgc
     2041 aacaggtacc atatttgttg ttggtatttc ttttatttca atttgtcctg attgagtaag
     2101 ataaggtcct gaggacaata atcctaatag aatattttta ttggcactga tatggtagtt
     2161 gcccactttg tcatgaaatc cttgtagaca aattaaaact ggtgaacctg aggagccagg
     2221 aaaacatgcc atatcaataa tacctacact cctcccatta aaatctaatg aaggatgtga
     2281 agctgttatg ccatttctaa caataggaaa attattgact tcatcccata aacctatcgg
     2341 ataaccaatc attaacactt tattatccac atcagattct tctgcgagct catcgtcatc
     2401 acgtatcaag gaatcatcaa atgaaaagtt aaaaatttct ttacctaatt tctcttttgc
     2461 atgatttctt attggaacaa ataataaagc tccgagatcg acttcatctt taggatgagg
     2521 tatccaaaga tttgcataat tctcaatagt tatagaaaaa gattgatttg ttggttcaaa
     2581 ttttttgtca tcagtaactg cttcgtgaaa ataaaaagat aattttctat tgcaattaat
     2641 aacatgttta tttgttaata taagttgtat ttgtttgtcc tgatctagtt ggatatgaaa
     2701 gaaaaaacct gtacctgttc cgttatctcc agatattcgt actgtgctat aaagtaattg
     2761 ttctgcaact gttcgtggtt gaattactct cataataaat gatcttcgta gctcttttag
     2821 tgtggcgtct cgcttaagtt aaataaaact catttgcagt ttttttgttt aaaataatct
     2881 tcaaatgata aaacattatc agagagtgct tcagcaatta ctgtttcaat ttcgttaaaa
     2941 ttcagatctc caacatctat atcaaatgga aatctccata attggctatt agtttgacga
     3001 tatttgctga tagtatgctt aattgtttgt tcatctaaat tagcatagcg agatagccag
     3061 gtattatata gtatttcagt tggaaatata tagtctctat cttgtttacg ttttctcggg
     3121 gtgtcgttgt gtcttaaatc gctaataaat aattcttctt cagttggtaa gtcatcttcc
     3181 catcgatact gttcatattg gagattcatt agtgcatcat tgatatgcgc ttgtactgag
     3241 cgatcgcctc gaggaacaaa tgcatattca tggtgatttt ctaagaatga ttccatgcta
     3301 gcatttaata atgtttcatc ttgtcctccc atattaataa cagaaataat atgaagactg
     3361 aatcggtcat gaaattgctc aaggaacttt tggatatttc ctttttttaa tccccaaaaa
     3421 atcatacaga aacgtgaatc tgtatccatt gctagtatga catttctgtg accaaatttt
     3481 ttaacatgaa ccatccagtg ccactgttga tgatcgtgca aaacaggttc ttcagaaagc
     3541 tcacacttag gtgttggtga catgggagat agtttgttac ctttacggat agttgtgaaa
     3601 aaatctgagg ctgccttagt acagttgaaa attaacataa gaaagatttt attgatttgg
     3661 gtttgagatt aacaaatagt aatgtctact taattttttg tctacattac gtctacatga
     3721 cttttttaaa ctttctgggg aattctaatc tgaagtgcga cagtccaaaa agctgtatca
     3781 tgttcttggg gaacttgaaa tagcaaagga gactgccaca tgggacagta tactcaatta
     3841 tctgtaacag atcgacgccg tttatttgtt tttc
//
LOCUS       DASLOE010000221         3859 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.222, whole genome shotgun sequence.
ACCESSION   DASLOE010000221 DASLOE010000000
VERSION     DASLOE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.222"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            185..961
                     /locus_tag="U6309_002748"
     CDS             185..961
                     /locus_tag="U6309_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506160.1"
                     /translation="MRGKREFLKHEYSPGHWSIDYTRAGTSIAVITVRNKYHYSVILN
                     PTDCRGYRIIIRYLNEGDSSLSSAFNRPYTVSEQRGLNDVASLMTQVYEKLGLIVQFS
                     QLGNNSQSFDKETGVTLIGSEEEPSMLHLHMWGRGDPDMEYIAGVPLRGPEPGLMFDL
                     IAKHKTHPINQHAIKWNEEELKACLAMFKLKLAEYVNSPEFTEEFGDTLKVTIHDKKD
                     LGEEKESGFGFFHKMKSSSVGMTTLEKTLALEDDKINPFV"
     gene            complement(1761..3761)
                     /gene="ceg14"
                     /locus_tag="U6309_002749"
     CDS             complement(1761..3761)
                     /gene="ceg14"
                     /locus_tag="U6309_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg14/sidL"
                     /protein_id="HEN5506161.1"
                     /translation="MQSLDEILKKLGEDLKKTSKETAEKLSVLSRKVAEDVVKVSNET
                     VQKINEVTKKFSVDTTVSTTDTQKILDDFMKNIRFSRFKQIWQAIEEAPKSKHILLKS
                     LFLKGLLTHNKPLLEEIIRQIELIPYSSDLEMLGAFSHDKAHPLSPELLEFVQEMLAN
                     ESEKVLLTILMNAFQSIPELSLDSKTGTLSMKTKKELLPLLISKVDTPIAKSIMSQLT
                     DISFDSVLPAFRPSIGDPSYQETKINLSKYLSLVGDKTDISEFLILTIGLFSSLKIAD
                     KGFLQELSADYLFVRYDDCLHKIIEKLKEKEVVEQLGEKQVQKIMEASGNLKRFKPTR
                     NDPRRFFETRLAQYINGLSHSEKTLEIDLTEESLEPEDEELRDNTLKACNKILQFFLG
                     DCGRVDTPREMEQKICIFANGYKDTSKFTKKEVEFITGVLHHAEAHKGRDRVERDKLT
                     GIKERKFETDSEKVGSDVWNCGGGVMKGCSPVFYDAQREPMRNMLVDSSTVAPDNKEG
                     KQWFWNNNRQYSSYVGSISGHTCNIVGMLAKYMENYKEDPDLQNDINLFLIQVIGVYA
                     KRGFHAMLEVIDVLHDPYVQDIFKGYGVQVNLYSYFKENPELAGFLQHAMNDATTYTQ
                     ALVNKKHVKEALKSNSFFSKKDEESGDKKNDHTPKDGTVYGC"
BASE COUNT         1202 a          742 c          691 g         1224 t
ORIGIN      
        1 caaattaaaa gttgccatat caatgacaaa ctgtaaaaac agtgacactt tgctattaaa
       61 acccattctg ggatatagtt gttttaattt ttctataagt gtctttgttt taattttgtt
      121 ttatatcccg ctgacatcag gcacatgttg ggcaaaaaaa agtgtaaatc acaatgaggt
      181 tgtaatgaga ggaaaaagag aatttttaaa acatgaatac tcccctggcc attggtcaat
      241 tgattatact cgggcaggca catcaatcgc agttatcact gtccgaaata aatatcatta
      301 ctctgtgatt cttaacccta ccgattgtag aggctatcgt atcataattc gatatctgaa
      361 tgagggtgat tcaagccttt catctgcttt taaccgccct tatactgtat ctgaacaacg
      421 tggattgaat gacgttgcaa gtttgatgac ccaagtctat gaaaaattag ggcttattgt
      481 gcaattttct caacttggca ataactccca gtctttcgat aaagagacgg gagtaacgct
      541 cattggaagt gaagaagaac ccagtatgct tcatttgcat atgtggggca ggggcgatcc
      601 tgacatggaa tatatcgctg gtgttcctct tagagggccc gaacctggtt tgatgtttga
      661 tttaattgca aagcataaaa ctcaccctat taaccaacat gccataaaat ggaatgaaga
      721 ggaactcaaa gcctgtcttg ccatgttcaa acttaaactg gctgaatacg ttaattcacc
      781 cgagtttact gaagaatttg gcgataccct aaaagtgact attcacgaca aaaaagatct
      841 tggcgaagag aaggaaagtg gttttgggtt tttccataaa atgaaatctt catcagttgg
      901 tatgacaact ttggaaaaaa ccttagcact ggaagatgat aaaataaatc ccttcgtata
      961 atcgacagag caacataaat ggtatcatct ttattgtact cgaatttcag ccaggaaaag
     1021 agtcaaaatt tccggataaa aatgttttca gccagcgact gttaaccacg tgcatccact
     1081 gtctacctgt tgctactagc aacttgatag tggataaact tatgaaaaac atatctgcgg
     1141 attttttatc tgttcgaatt caatttatca acagctcaat tgatgttcaa caaaaaacaa
     1201 actgatgaat atgtttaaag cgcttgtaca cacgccatcc agagtggtat ttattggcat
     1261 taggcctctg gcataacctg cgtattttgt ttcagggcaa ggggcaaacg tctttaaata
     1321 agcgccacag aaaagttcgc aacgcggcag taaaataaaa ataacaggtt atgccaagca
     1381 atctattcgt ctcggcaaag agtgcatcaa cacatataac gaagtacaag ctataggtct
     1441 atagagaagt tgcttgtctt taaaaaaatt taaaaacgag ctagacaaag aacaggactt
     1501 acgaaaaact ccaaggtcaa ggcaaaaaat gtttttaatg agggagttta gatagactaa
     1561 atgaccgaat taaaaacatt ttttaacata gagattgggg tttttcgtaa gtcctgtaga
     1621 aattatattt gagtgcaaaa atcaatcttg ctcaggatga tttgttcgaa aagatatatt
     1681 cttaatgcaa aatttccctg aatttgatgc ctcgggcaag ccgaggctag tagagaatgg
     1741 gtgggatctc aaattatcct ttagcaccca taaacagttc catcttttgg cgtatggtca
     1801 tttttcttat ctccactctc ttcgtctttc ttgctgaaga atgaatttga tttgagtgct
     1861 tccttaacat gcttcttatt cacaagagcc tgagtatacg tggttgcatc attcatcgca
     1921 tgctgcagga atccagcaag ctcagggttt tctttaaaat agctataaag gttaacttga
     1981 acaccatatc ctttgaatat gtcttgtaca taaggatcat gaagaacatc aatgacctca
     2041 agcatggcat gaaaaccgcg cttggcataa acacctatga cctgaataag aaaaagattg
     2101 atatcgtttt gcagatcggg atcttcctta taattttcca tatacttggc taacataccc
     2161 acaatattac aagtatgtcc ggaaatcgat cctacgtaag acgaatactg acggttatta
     2221 ttccaaaacc attgttttcc ctctttgtta tcaggagcaa cagttgaaga atccactagc
     2281 atattacgca tgggttcgcg ttgggcatca taaaagacag gcgaacagcc tttcatgact
     2341 ccaccgccac aattccatac atccgaacct actttttctg aatcagtctc aaattttctt
     2401 tctttaatgc cagtcaattt gtcacgttca acccgatcgc gccctttgtg cgcttcagca
     2461 tgatgtaata caccggtaat aaattcaact tctttcttgg tgaatttaga ggtatcctta
     2521 tagccattgg caaagataca aattttttgt tccatttctc gtggagtatc cactcttcca
     2581 caatcaccta aaaagaattg cagaatttta ttacatgctt tcagtgtgtt atcccttaac
     2641 tcctcatctt ccggctccag actctcttct gttaaatcga tttcaagggt tttttcagag
     2701 tgggacagcc cattaatata ctgagcaagg cgagtttcaa aaaatcgtct tgggtcatta
     2761 cgtgtcggtt taaaccgctt aaggtttcca gaagcttcca tgattttttg aacttgtttt
     2821 tcccctaatt gttctactac ttctttttcc tttagttttt ctattatttt atgcaagcag
     2881 tcatcatatc gaacaaataa ataatcagcc gataattctt gcaaaaaccc cttatctgca
     2941 atttttaagg aggaaaaaag accaatcgtt aatattaaaa actctgaaat atctgtttta
     3001 tcacctacaa gagacaagta tttactcaaa tttattttcg tttcctgata gctgggatca
     3061 ccaatcgatg gacgaaatgc aggtaatact gaatcaaatg agatatcagt taattgtgac
     3121 atgattgatt ttgcaatagg ggtatcgact tttgaaatta aaagaggcag taactctttt
     3181 ttggttttca tgcttaaagt ccctgtcttg ctatcaagag acaactcagg gatagactgg
     3241 aaagcattca tcagaatagt gagcaatact ttctcacttt cattggcaag catctcttgc
     3301 acaaactcca gaagctcagg tgagagcgga tgtgctttgt catgactaaa agcaccgagc
     3361 atctccagat cacttgagta aggtattaat tcaatttgtc gtataatttc ttcaagtaga
     3421 ggtttattat gagtcaatag cccttttaag aagagagatt ttaacaaaat atgcttggat
     3481 ttcggtgctt cctcaattgc ttgccaaatc tgcttaaatc gagaaaaacg tatatttttc
     3541 ataaaatcat ctaaaatttt ttgggtgtca gtagtgctga ctgtagtatc tacggagaat
     3601 tttttggtta cttcattaat tttttgaacc gtttcattgg ataccttaac cacatcttca
     3661 gcaactttcc ttgataaaac actgagcttc tcagccgttt ccttcgaagt ctttttcaga
     3721 tcttctccca gtttctttag aatctcatct aagctttgca tataaactct acctcttaac
     3781 attgtcttgc ctgacaactt atatttcaca gggggtatga taaaacacaa agattaagaa
     3841 aatattaaac ttattgttc
//
LOCUS       DASLOE010000222         3821 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.223, whole genome shotgun sequence.
ACCESSION   DASLOE010000222 DASLOE010000000
VERSION     DASLOE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.223"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            98..232
                     /gene="rpmH"
                     /locus_tag="U6309_002750"
     CDS             98..232
                     /gene="rpmH"
                     /locus_tag="U6309_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEN5506162.1"
                     /translation="MKRTFQPSNLKRKRDHGFRLRMSTRAGRLVIKRRRAKGRKRLSA
                     "
     gene            233..577
                     /gene="rnpA"
                     /locus_tag="U6309_002751"
     CDS             233..577
                     /gene="rnpA"
                     /locus_tag="U6309_002751"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEN5506163.1"
                     /translation="MFAFKKAQRLLKKNDFDFVFESAKKITTDDFIFLFRENKLGYAR
                     LGLALSKKMIAKAHDRNRIKRLLRESFRHTNLPAVDIIILARPGLAKKTNLGINTKLN
                     KTWEKLASCYGK"
     gene            544..789
                     /gene="yidD"
                     /locus_tag="U6309_002752"
     CDS             544..789
                     /gene="yidD"
                     /locus_tag="U6309_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEN5506164.1"
                     /translation="MGKISLMLRQIVCLPIKMYQYFISPLITPCCRYYPSCSEYADSA
                     IKHYGVIKGLLMALNRLSRCHPWSKGGYDPLFPNDKN"
     gene            799..2469
                     /gene="yidC"
                     /locus_tag="U6309_002753"
     CDS             799..2469
                     /gene="yidC"
                     /locus_tag="U6309_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN5506165.1"
                     /translation="MDIRRIVLYMALALIGLSLWNAWQIDYPAKQPVEEKTASQLTSD
                     GHLLPQIIPSNAEQPVTLKAEEKASSGKQLIQVKTDVLDVDIDLKNGDIVKGLLLDYP
                     LSVEDKNKPFPLLQNQASQRYVANSSLFVLDGQTPQSLDFDFTSEKEYYELKPDQNQL
                     IVTLNGKSEDGLDVKKEFVFTKGSYLIEVNYKIANTGNSLWKGYFNTQLLRSSPKEDK
                     SSIFHIGSYTGASFSNPGKNRYQKVSFSDMSKSNLDVDAKGGWIAMQQHYFLSAWVPN
                     ADSENKFYTLATDKDYTIGAVSQPITVKPKEDKIVGSKLYIGPEITSVLKGISPSLDL
                     TVDYGILWFLSSLLFSLMKAIYTVVGNWGWSIVLVTVLIKLAFYRLSATSYKSMASMR
                     KLQPKLQALRERYGDDKAKISQATMELYKQEKVNPLGGCLPILIQIPVFIALYWVLLE
                     SVELRQAPFIFWINDLASADPYHVLPLIMGATMLIQQKLNPAPADPMQAKVMMFLPIL
                     FTGLFWNFPSGLVLYWIVNNTLSILQQWYITRKYSDEKPAKKVVATAK"
     gene            2470..3810
                     /gene="mnmE"
                     /locus_tag="U6309_002754"
     CDS             2470..3810
                     /gene="mnmE"
                     /locus_tag="U6309_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEN5506166.1"
                     /translation="MSIDTIVAIATPPGRGGVGIVRISGPNAYAIALCLNGNKALQPR
                     LATFCSLYKGNNEVLDQGLVLYFKGPHSFTGEDVIEIQAHGSPVVLDLLIKESIAAGA
                     RLARPGEFSERAFLNDKIDLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNE
                     ELIYLRMYVEAAIDFPEEEIDFLNDGNVSQLLQKIIGRLEDIRSQANQGVLLREGLSL
                     VIAGRPNAGKSTLINNLAGRDVAIVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDS
                     DDLVEKEGIKRAWQELKRADCVLLVVDINNPDQQNSLLNELRLTLPNKIPIITVYNKI
                     DTTKLTAKCDEHTVYLSAKTGEGLDELKKVIKQVVGYQPTEGQFLARRRHLQALDEAK
                     ALLLTGQSQLTNHKAGELLAEDLRLAHQTLCEITGEFTSDDLLGKIFSSFCIGK"
BASE COUNT         1271 a          633 c          764 g         1153 t
ORIGIN      
        1 taaaaaatta cacaaaaaat aatcagttat tcaaattgac agttgaaaaa aagttatcta
       61 tcattgccaa cctcataaat ttagaagcag ggttatcatg aaacgcactt ttcaacctag
      121 taatttaaaa cgtaaacgcg atcatggttt tcgtttaaga atgtctactc gagctggtag
      181 attagttata aaaagacgtc gtgctaaagg tcgtaagcgt ttatctgcct aagtgtttgc
      241 atttaaaaaa gcgcaacggc tattaaaaaa aaatgatttt gatttcgtgt ttgaaagcgc
      301 gaaaaaaata actacagatg actttatttt ccttttcaga gaaaataagt taggctatgc
      361 acgccttggt ttagcattgt caaaaaaaat gatagctaag gctcatgaca gaaatcgcat
      421 taaacggcta cttagagaaa gttttcgtca tactaacttg cctgctgtag atataatcat
      481 tttggcaaga ccaggtctag caaagaaaac caatttaggt ataaatacta aattaaataa
      541 aacatgggaa aaattagcct catgctacgg caaatagttt gcttaccaat aaaaatgtat
      601 cagtatttta ttagcccttt gataacacca tgttgtcgct attatccgag ttgttcagag
      661 tatgctgaca gtgcgatcaa acattatggc gtaattaaag gtttattgat ggcacttaac
      721 agattgtctc gttgccatcc atggtcaaaa ggtggctatg atcctttatt tcctaacgat
      781 aagaattgag aatatttaat ggatatacga cgtatagttt tatatatggc gcttgcgctt
      841 attggtttat cactttggaa cgcttggcaa attgattatc cagccaaaca accagttgaa
      901 gagaagacag caagccaatt aacatcagat gggcatttgt taccacaaat aattccttct
      961 aatgcagagc aacccgtaac tttgaaagca gaggaaaaag caagcagcgg taaacaattg
     1021 attcaggtta aaacagatgt tttggatgtc gacattgatt taaaaaatgg agatattgtt
     1081 aaaggtttat tgcttgatta tccactaagt gttgaagata aaaataaacc attccctctc
     1141 ttgcaaaatc aggccagcca gagatatgta gcgaacagta gcctttttgt tttagatggc
     1201 cagacaccac aatcattgga ttttgatttt acatcagaaa aagaatatta cgaattgaaa
     1261 cctgatcaaa accagctgat agtcactcta aatggcaaaa gcgaagacgg tttggatgtg
     1321 aaaaaagaat ttgtttttac aaagggaagt tatttaatcg aagtcaatta caagatagcc
     1381 aataccggta attcgttatg gaaagggtat tttaataccc agttattgcg cagttctccc
     1441 aaagaagaca aatcgagtat ttttcatatt ggctcgtata caggtgcttc cttttcaaat
     1501 cctggaaaaa atcgttacca aaaagttagt ttcagtgaca tgagcaaatc taacctggat
     1561 gtggatgcaa aaggtggttg gattgctatg caacaacact attttttgag tgcttgggtt
     1621 cccaatgcgg atagtgaaaa caagttttat acattagcta cggataaaga ttatactatt
     1681 ggagcagtaa gccagcctat tacggtcaaa ccaaaagaag ataaaattgt aggatccaaa
     1741 ttatacatag gaccagaaat taccagtgtt ttaaaaggca tatccccatc tctggattta
     1801 acggtggact atggcatttt gtggttcttg tcttcgttat tattttcttt aatgaaagca
     1861 atttatactg ttgttgggaa ttggggctgg tcaattgttc ttgtgactgt cctaattaaa
     1921 ttggcatttt atcgattatc agctacaagc tataaatcca tggctagtat gcggaagttg
     1981 cagcctaagt tgcaagcttt gcgtgagcga tatggtgatg ataaagctaa aataagccaa
     2041 gctaccatgg aactttataa gcaagaaaaa gtaaatcctt taggcggctg tttacctata
     2101 ttaattcaaa ttccagtttt tatagcgctc tattgggttt tactggaaag tgttgagtta
     2161 agacaagctc catttatatt ttggataaat gatttggctt cagcagatcc ttaccatgtt
     2221 cttccattga tcatgggagc aaccatgttg attcagcaaa agctcaatcc agcaccagct
     2281 gatccaatgc aagctaaggt aatgatgttt ttacccatat tatttaccgg actgttctgg
     2341 aattttcctt ctggtctggt tttgtactgg attgtgaata atactttatc aatattacag
     2401 caatggtaca tcaccagaaa atactcagat gaaaagccag ctaaaaaggt tgttgccaca
     2461 gcaaaataaa tgtcaataga taccattgtt gctatcgcta ccccgccagg acgaggcggg
     2521 gtaggaatcg tcagaatatc aggtccaaat gcttatgcta tagccctttg tctaaatggg
     2581 aacaaagcat tgcaaccacg tttagccact ttttgctctt tatataaagg aaataatgaa
     2641 gtccttgatc aaggattggt tctttatttt aaaggacctc attcttttac aggtgaggat
     2701 gttattgaaa ttcaagcgca tggctcacct gtagttttgg atttattaat taaggaatcc
     2761 atagcagcag gcgcaaggct agccaggccg ggtgagtttt ctgaaagagc atttcttaat
     2821 gataaaatcg atttaattca agcagaagcc atagctgatt taattcaagc cagttcagat
     2881 actgcggctc gtatggcttt aaaatcactg caaggtgatt tttctaaaaa aataaatcaa
     2941 ttgaatgaag aactaattta cttacgcatg tatgttgaag ctgctatcga ttttcctgaa
     3001 gaggaaattg attttcttaa tgatggaaat gtcagccaat tactacaaaa aattattgga
     3061 cgattagaag atatacgttc acaagcgaat caaggagttt tgttgcgtga agggttatcc
     3121 ttggttattg ctggcaggcc taatgcagga aaatcaacct taattaacaa cctggcaggg
     3181 cgggatgttg ccattgttac tgaaattgcc ggtactacgc gagatattat gcgagaacat
     3241 atcctattgg atgatatccc tctacacatt atcgatacgg caggattaag agacagcgat
     3301 gatctggtgg agaaagaggg aataaaacgt gcttggcaag agttgaagcg agctgattgt
     3361 gtgcttcttg tagtagatat taacaatcct gatcaacaaa attctctgct taatgaattg
     3421 agattaacat tacccaataa aatacccata atcacggtat acaataaaat tgatactact
     3481 aaacttacag caaaatgtga tgagcataca gtttatttgt ctgcaaaaac tggtgaggga
     3541 ctggatgagc taaaaaaggt gattaaacaa gtggttggat atcaaccaac agaaggccaa
     3601 tttttagctc gcagacgaca tctacaagcg ttggatgaag caaaagcttt gttactaact
     3661 gggcaatcgc agctaacaaa tcataaagct ggagaattgt tggcagaaga tttacggtta
     3721 gctcatcaga ctttatgtga aattacaggt gagttcactt cagacgattt attaggaaaa
     3781 attttttcca gtttttgtat tggaaaataa cgatgatgca a
//
LOCUS       DASLOE010000223         3776 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.224, whole genome shotgun sequence.
ACCESSION   DASLOE010000223 DASLOE010000000
VERSION     DASLOE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.224"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..689)
                     /locus_tag="U6309_002755"
     CDS             complement(<1..689)
                     /locus_tag="U6309_002755"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEN5506167.1"
                     /translation="MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQL
                     NDLGIPIIRIPHQGYQLEKPLILLDSDEITKQLHSKGFSQPFNLHLFTSIDSTNRYLK
                     DLPLSSAVEICCTEMQTQGRGRFGRHWHSPFGENIYCSTRWSFSCDISKLSGLSLVIS
                     LATVLTINEFIVSDSDKVKIKWPNDILWNNKKLCGILVEIIAESNATAQFIIGVGLNI
                     NSDTKNNPLPD"
     gene            complement(693..1430)
                     /locus_tag="U6309_002756"
     CDS             complement(693..1430)
                     /locus_tag="U6309_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEN5506168.1"
                     /translation="MIITGFTPLNTQNCILNDSRIDLWQFSLDYELHSAYQLLNADER
                     SRADRFYFNRHKRRFTNARATLRIILARYLNTPPERLEFTYNAHGKPNVINSQKLQFN
                     ISHTGEMAILAVGKTYPIGVDIERYSARPYEGIGKNLFSEQEYQELKKAHQSLKPALF
                     FHIWAQKEAFIKASGLGLSYPTKEFHVPTNFPTKQLVDDPLHNMTWQLRSFMPEVCCS
                     GALCYHPSVREIRHGIVQLQQNISLKF"
     gene            1512..2465
                     /locus_tag="U6309_002757"
     CDS             1512..2465
                     /locus_tag="U6309_002757"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEN5506169.1"
                     /translation="MTRQFLEFEQPIEELNQKIEALRMVGSDNEVNLSEEIARLEAKC
                     SELTENIFSRLEPWQIAQMARHPLRPQTTDYIERIFTDFQELHGDRSYSSAPAIIGGM
                     ARLNGEPVMVLGHQKGKRTKEKVYRNFGMARPEEYRKALRLMRMAEKFKMPVVTFIDT
                     AGAYPGIGAEERNQSEAIARNLFAMSRIKTPIVCIVTGEAGSGGALAIGVGDKIIMLQ
                     FSIYSVISPEGCASILWKDASKASEAARAMGITADRIFENELVDMVVPEPLGGAHRDV
                     DEMASRLKRLLTSELQALKKVPLDQLIELRYQKFMAMGACD"
     gene            2458..3753
                     /gene="tilS"
                     /locus_tag="U6309_002758"
     CDS             2458..3753
                     /gene="tilS"
                     /locus_tag="U6309_002758"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEN5506170.1"
                     /translation="MTRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLY
                     NQLLAVHINHGISGNSLNWQRHCEQLCLDLGIAFIAKAIEFDRSANVEEAARNARYDF
                     FSSLLEDNDCLVLGHHLDDQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFLTC
                     PRVELEHYARIHELKWIEDESNQDTKYSRNYLRHRVMPLLLEKWPGVVGNISRAATHC
                     QQAKANLEVLAIKDCPDLLNATDHLLIEPLKGLEFERITNVLKFWLKKNRVQLPSSKT
                     FHRIVHEIIFAKLDAMPTVSWDQIQVRRFQQHLYLLKADQINLPKAIKWQQFPASLTY
                     PDADIKLSAVKAQKGLMIPKDAQIEIRFRKGGEEIYWHGQTKHLKKLFQEWQIPPWVR
                     DRVPLVYINDQLACVVGYAVSDVFFTTNPLEAWSIVNNS"
BASE COUNT         1108 a          686 c          839 g         1143 t
ORIGIN      
        1 ttatctggta gaggattgtt ttttgtatca gagttgatat ttaaaccaac acctataata
       61 aattgtgctg ttgcgttaga ttctgcaata atctctacta aaatcccaca taacttttta
      121 ttattccaca ggatatcatt tggccatttt atttttactt tatcagaatc agatacaatg
      181 aattcattta tagtgagaac tgttgctaaa cttatgacta aactgagacc cgatagctta
      241 gatatgtcac aactaaaact ccaccgggta gagcagtaaa tattctctcc aaaaggagag
      301 tgccagtgtc gtccaaagcg ccctctccct tgagtttgca tttcagtgca gcaaatttcg
      361 acggctgagc ttaagggcag atctttcagg tagcggttgg ttgagtcgat cgatgtaaat
      421 aaatgcaggt taaacggttg gctaaatcct ttgctgtgta attgcttggt aatttcatca
      481 gagtctagca ggattaaagg cttttccaat tggtatcctt gatgtggtat gcggataata
      541 ggtatgccta aatcattaag ctgattgatt tgtttccata ctgctgagcg actgattttt
      601 aaagcgtttc ctaattcact cccactatga catgccccat cgcccagtat ttgcattaaa
      661 gtatgttgag aagtattaaa ttgaaacatg atttaaaatt ttaaactaat attttgttga
      721 agctggacga tcccatgacg aatttctcta acagaagggt ggtagcataa agcgccactg
      781 cagcatactt ccggcatgaa tgagcgtagt tgccaggtca tgttatgcaa gggatcgtca
      841 accaattgct ttgtagggaa attggtggga acatgaaatt ctttggttgg ataggagaga
      901 cctaaaccac tggctttgat aaacgcttcc ttttgagccc atatatgaaa aaataaggct
      961 ggcttaaggg attgatgtgc ttttttcagt tcctggtatt cttgctcgga aaacaaattt
     1021 ttaccaatcc cttcataagg tcgggctgaa tatctttcaa tatccactcc aatcggatag
     1081 gttttaccca ctgccaatat ggccatttca cccgtatgac tgatgttaaa ttgcaatttt
     1141 tgcgaattaa tgacattggg cttgccatgg gcattgtaag taaattccaa tcgttcaggg
     1201 ggggtattaa ggtatcgggc caggataatt cttaaagttg ctctggcgtt ggtaaagcgt
     1261 cttttatggc gattaaaata aaagcgatca gcacgggagc gttcatcggc attcaataac
     1321 tgatacgcac tatgcaattc ataatctaat gagaactgcc aaagatcaat acgtgaatca
     1381 tttaaaatgc agttttgggt gtttaatgga gtaaatcctg taattatcat ctgttgtatc
     1441 tgaaatctgg catcgattaa cggttaagag tatcatatac caaagatgtt taattcacca
     1501 aagagtaatc aatgacccga caatttttgg aatttgaaca acctattgaa gagttgaatc
     1561 aaaaaattga agcactgcgt atggttggca gtgataatga agttaattta agtgaagaga
     1621 tagctcgact tgaagcaaaa tgctccgagt taacagagaa tatattctcc aggctcgaac
     1681 catggcaaat cgctcagatg gctagacatc ctttaaggcc acagaccact gactatattg
     1741 agcgaatttt tactgatttt caggagttgc atggtgatcg cagttattct tcagcccctg
     1801 caattatcgg aggcatggcg cgattaaacg gggagcctgt catggtactt ggtcaccaaa
     1861 aaggtaaacg taccaaggaa aaagtataca gaaactttgg tatggcaaga cctgaagaat
     1921 acaggaaagc actgcgtttg atgaggatgg ctgaaaaatt caaaatgcct gtagtcacat
     1981 ttattgatac ggctggagct tatcctggca taggtgctga agaacgtaac caatcagaag
     2041 ccattgcacg gaatcttttt gctatgtctc gcattaaaac accaattgtc tgtattgtta
     2101 caggagaagc cggttcaggt ggtgccttgg cgattggggt cggtgataag attattatgc
     2161 tgcaatttag catttattcc gtcatttctc ctgaggggtg cgcctcaatc ctttggaaag
     2221 acgcctctaa agcgagtgaa gctgcgcgtg ctatgggaat tactgcagac agaatattcg
     2281 aaaatgaatt ggttgatatg gtagttcctg agcctttggg gggcgcacac cgagacgttg
     2341 atgagatggc aagtcgcttg aagagattat taacgagtga actccaggct ttgaaaaagg
     2401 tgcctctgga tcaattaatt gagttaaggt atcaaaagtt tatggcaatg ggtgcttgtg
     2461 actaggcctt tactaagtcc cgaatgggta gctcgcctta aacaattcaa gaaattgata
     2521 gtagggttca gtggtggtct ggactctaca gtgctcctgc atgtccttgc ctcaactcct
     2581 cccttgtata atcaactttt ggctgtccat attaaccatg gcattagtgg aaattctctg
     2641 aactggcaaa ggcattgtga gcaattgtgc ctggatttag gtattgcttt tattgccaaa
     2701 gcgattgaat ttgatcgttc tgcaaatgtt gaagaagcag cacgcaatgc gagatatgat
     2761 tttttttcct ctttattaga ggataatgat tgtctggttt tggggcatca tcttgatgat
     2821 caggccgaaa cagttctttt gcaactgttt cgcggtgcag gagttgatgg cttggcagct
     2881 atgcaggaat gcagcaattt gggcgccggg caattagcaa gaccattttt gacctgcccc
     2941 agagtggaat tagagcatta tgctcgcatt catgaattga aatggataga agacgaaagc
     3001 aatcaagata caaaatattc tcgcaattat ctgcgccatc gagtaatgcc attattgctt
     3061 gaaaagtggc cgggagtcgt aggtaatatt tcccgagctg cgactcattg tcagcaggca
     3121 aaagcgaatc ttgaggtatt ggctataaag gattgccctg atttgttaaa cgcaactgat
     3181 catttgctga ttgaacctct aaaaggactt gaatttgagc gtattacaaa tgttttaaaa
     3241 ttttggctta aaaaaaatag agttcaattg ccttcgtcca agacttttca tcgtattgtt
     3301 catgaaataa tttttgcaaa gctcgatgcc atgccgacag tgagttggga tcaaatacaa
     3361 gtacgtcgtt tccaacaaca tctttatttg cttaaggcag accagatcaa tttaccgaag
     3421 gctattaaat ggcaacaatt tccagcttct ttaacttatc cagacgctga tattaagttg
     3481 tctgcagtaa aggcccaaaa gggattaatg atcccgaaag atgcgcaaat agaaatacga
     3541 ttcagaaagg gtggtgaaga gatttattgg catggtcaaa ccaaacatct aaaaaaacta
     3601 tttcaggaat ggcaaattcc cccatgggta agggatagag ttccattggt ttacattaat
     3661 gatcaattgg cttgtgtagt aggttatgca gtgagcgatg tgttttttac tactaatcct
     3721 ctggaagcct ggtctatagt taataattct taatatcaac aatctccgct ccctta
//
LOCUS       DASLOE010000224         3758 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.225, whole genome shotgun sequence.
ACCESSION   DASLOE010000224 DASLOE010000000
VERSION     DASLOE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.225"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..387
                     /locus_tag="U6309_002759"
     CDS             <1..387
                     /locus_tag="U6309_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444483.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HEN5506171.1"
                     /translation="KDEPRRKVTFEYVMLKGVNDQPEHASQLIKLLHNVPAKVNLIPF
                     NPFPLTQYQRSSRETIDAFRDKLMKHGINTITRKTRGDDIDAACGQLAGEVKDKTSRS
                     QRWQKLHFISKTDKSTELTISSEEIA"
     gene            567..1349
                     /gene="pilW"
                     /locus_tag="U6309_002760"
     CDS             567..1349
                     /gene="pilW"
                     /locus_tag="U6309_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEN5506172.1"
                     /translation="MLKSIRYLLIIIICLFVGACQHTQDNEEPNTIRKVNLSKAASFN
                     VQLGLGYLKQGDRPRAKKKLLTALEQQPDSADVNAALAYYFEQTKELEQAKKFYHKAI
                     TLSQNGGAQLNNYGAFLCRQGDYKNAETYFLKAVKDQNYVHTSGAYENAGLCAMAIPD
                     HDKAILYFTKALNQDPSRKESLYELVKLQSKIGHDKEALELLQKHADLVLNDKVMLAL
                     AKDIANRTGQYTLAAEYENSFNKLEQTNNISGVNDEYNSSNG"
     gene            1324..1893
                     /locus_tag="U6309_002761"
     CDS             1324..1893
                     /locus_tag="U6309_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN5506173.1"
                     /translation="MNTIAAMDETRNNEFTNPGYELAALRQQKGYSIEYVASKLHLRA
                     RIIELIEAGDFDLLPQPVFVKGYLRAYSKLLGVSPEPFLTVFNAQYCFEKKPERAALW
                     QSKKESHKAEHFIKWFTILFAIGVLVAVGIWWQKNKDSQSIDTPKESSSGLSISETSS
                     EIKLTDLSKMESLLTPNNTQMTPLEKNGG"
     gene            1883..3163
                     /gene="hisS"
                     /locus_tag="U6309_002762"
     CDS             1883..3163
                     /gene="hisS"
                     /locus_tag="U6309_002762"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEN5506174.1"
                     /translation="MVVDKIQAIRGMNDVLPDSTSVWRFIEQTFINCLVRYGYKEIRF
                     PIVENTQLFKRTIGEITDIVEKEMYTFNDLNGDSITLRPEGTAGCVRACIEHGLLHNQ
                     QQKLWYLGPMFRHERPQKGRYRQFNQFGVEALGITGTAIELELISICRRLWTDLGFSQ
                     SVQLQVNSLGEINERQKYRSILVEYLRDHFHILDEDSKRRLDKNPLRVLDSKNPDLQQ
                     LIQNAPKLIDVLGDDSREHFQSFCNGLETLGIPYSINPVLVRGLDYYGQTVFEWVTDR
                     LGSQATICAGGRYDMLVEFLGGASTPAVGFALGLERIFLLMETLNLLNESNNKQSIFI
                     IATNEEAILKALVMAESIRNAHPSLDVITNTAGGGFKSQFKKADKSGARMALILGEDE
                     IAREYVSIKDLRTEIEQTSIPMTKINEFLQNYLA"
     gene            3181..>3758
                     /locus_tag="U6309_002763"
     CDS             3181..>3758
                     /locus_tag="U6309_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN5506175.1"
                     /translation="MSVYMTEEEQLEIIKKWWKRYGNMITIFLSIVLLIIAGYRYMNW
                     HNDKIKQQASIAYENMMIAFSNQNIKSVRSYANELIKNYNHTVYADIAHMTLAKIYVS
                     KNKLEQARNELKAVASESQMTPLRQIAKIRIARLLAADKAYTNALNELSAVEDEAYLP
                     VINELKGDIYSAKGQYQDAMNAYRLAIDEVRT"
BASE COUNT         1330 a          629 c          747 g         1052 t
ORIGIN      
        1 aaagacgaac caagaagaaa agttactttt gaatacgtca tgctaaaggg ggttaatgat
       61 caacctgaac atgctagtca attaattaaa cttttgcata atgtgcctgc aaaagttaat
      121 ttaattcctt ttaacccatt tcctttaact caatatcaga gatcatcccg ggaaacaatt
      181 gatgcctttc gagataaatt aatgaaacat ggtattaata ctattactcg caaaactcgt
      241 ggagatgata ttgatgcagc atgcggccaa cttgctggcg aggtaaaaga taaaacgagc
      301 agatcacaac gatggcaaaa acttcatttc ataagcaaaa cagacaaatc aactgaattg
      361 accatttctt cggaagaaat tgcataacac agtcttgtat aataactgag aaccaattaa
      421 gaactttttc cagtattcct ggttggagat tatgtataat aaaggtgttt tcacctgcag
      481 actcacatta aatagtgaca aatcattttt aatttggact taagtggtta taatgcgaaa
      541 tcatttctaa cgtaaagtga ttaatcgtgc taaaaagcat aaggtactta ttaattataa
      601 taatttgttt atttgttgga gcttgtcagc atacacaaga taatgaagag cctaatacca
      661 ttagaaaagt aaaccttagc aaagcggctt cttttaatgt gcaattaggg ttgggatatt
      721 tgaagcaagg cgacagacct agagcaaaaa agaagctgct aaccgccttg gaacagcaac
      781 cagattcagc ggatgttaat gcagcattag cctattattt tgaacaaacc aaagaattgg
      841 agcaagcgaa aaaattctat cataaagcaa taaccctatc gcaaaatggt ggtgcacaat
      901 tgaataatta cggcgctttt ctatgtcgac agggggatta taaaaatgct gaaacttact
      961 tcctgaaggc cgttaaagat caaaactatg tgcatacctc aggcgcttat gaaaatgctg
     1021 gtttatgtgc tatggctata cctgatcatg ataaagccat tttatatttt acgaaggcct
     1081 taaatcagga tccatcgaga aaagaatcac tatatgaatt agtaaaatta caaagtaaaa
     1141 taggacatga taaagaagcc ctcgaactgc ttcaaaaaca tgctgattta gtattaaatg
     1201 ataaggtaat gcttgcatta gcgaaagaca tagcaaatag aacaggccaa tatactcttg
     1261 cagcagaata tgaaaatagc ttcaataaac tggagcaaac taataatatt agtggagtaa
     1321 acgatgaata caatagcagc aatggatgag acaagaaata atgaatttac caatcccggc
     1381 tacgagctgg cggctcttcg ccagcaaaaa gggtattcaa tcgagtatgt tgcgagcaaa
     1441 cttcatttac gagctcgtat tatagagctc attgaagcag gagattttga tttattgcct
     1501 cagccagttt ttgtaaaagg gtacttgcgc gcctattcaa aattattagg agtttcacct
     1561 gaaccttttt taaccgtttt taatgcacaa tattgttttg aaaaaaaacc ggaaagagca
     1621 gccctatggc aaagtaaaaa agagtctcat aaagcagaac attttataaa atggttcacc
     1681 attttatttg cgattggagt tttagtcgca gttgggatat ggtggcaaaa aaacaaggat
     1741 agccagtcta tagatacccc aaaagagagt tcatctggat tatcaatcag tgaaaccagt
     1801 tcggaaatta aattgaccga tttatcaaaa atggaatcgt tattaacgcc caataataca
     1861 caaatgacgc ctttggagaa aaatggtggt tgataaaatt caagctatcc gaggaatgaa
     1921 tgatgtattg cctgatagta cctcggtatg gcgctttata gaacagactt ttataaattg
     1981 cctcgtacga tatggatata aggaaattcg ttttccaatt gttgagaata cacagctatt
     2041 taaacgtaca attggtgaaa taacagatat cgttgaaaaa gaaatgtaca cattcaacga
     2101 tttgaatggt gatagcataa ccctcaggcc agaaggaaca gctggctgtg ttcgtgcctg
     2161 tatagaacat ggtttattgc ataatcaaca acaaaaatta tggtacttag gccctatgtt
     2221 tcgtcatgaa cgtcctcaga agggtagata ccgtcaattt aaccaatttg gtgttgaggc
     2281 gctaggaatt actggcactg ccattgagct tgagctgatc tcaatttgcc gcagattatg
     2341 gaccgacctg ggtttttctc agtcagttca attacaagtt aattcattag gtgaaataaa
     2401 tgaacgtcag aaatatcgct ctatcttggt agagtatcta cgcgatcatt ttcatatact
     2461 tgatgaagac agtaagaggc gattggataa aaaccctttg agagttcttg atagtaaaaa
     2521 ccctgacctt caacaactca ttcaaaatgc ccctaagtta attgatgttc ttggtgatga
     2581 tagcagagag cactttcaat cgttctgcaa cggattggaa acacttggta ttccttattc
     2641 catcaaccct gtattggtaa gaggattaga ttattatggc caaacggtgt ttgaatgggt
     2701 aacagaccga ttaggaagtc aagctacaat atgtgcagga ggcagatatg atatgttggt
     2761 ggagttcctt ggcggagcat ccacacctgc agtggggttt gctttaggat tggaaagaat
     2821 atttctttta atggaaactt taaacttgtt gaatgaaagt aacaacaagc aatcaatatt
     2881 tatcattgct actaatgaag aggccatatt gaaagcgctt gtcatggcag agtcaattcg
     2941 aaatgctcat ccatctcttg atgtcattac caatacggca ggcggtggct ttaaaagcca
     3001 atttaaaaag gccgataaaa gcggggctcg catggctttg attcttgggg aagacgaaat
     3061 agccagggaa tatgtaagca ttaaagattt gagaactgaa atagaacaaa catcaatacc
     3121 gatgaccaaa ataaatgaat ttttacaaaa ttatctagct taagggcagg agaatattat
     3181 atgtctgttt acatgacgga agaagaacaa ttagagatta taaaaaaatg gtggaagcga
     3241 tatggcaata tgataactat ttttttatca atagtgctcc tgattattgc agggtacaga
     3301 tatatgaatt ggcacaatga caagataaaa caacaagcat ctattgccta cgaaaatatg
     3361 atgattgcat tttccaatca aaatataaag tctgttcgtt cctatgccaa tgaattaatt
     3421 aagaattata accatactgt ttatgctgat atagctcata tgacacttgc aaaaatctac
     3481 gtaagtaaaa ataaacttga gcaagcaaga aatgaattaa aggctgttgc atcggaaagc
     3541 caaatgacac cgctaagaca aattgccaaa atccgcattg cacgtttact ggcggcagat
     3601 aaagcttata ccaatgcatt aaatgaatta agtgctgttg aggacgaagc ctacttacca
     3661 gtaattaacg aactgaaagg ggatatttat agcgccaaag gccaatatca ggacgcgatg
     3721 aatgcctacc gacttgcaat agatgaagta agaacaaa
//
LOCUS       DASLOE010000225         3753 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.226, whole genome shotgun sequence.
ACCESSION   DASLOE010000225 DASLOE010000000
VERSION     DASLOE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.226"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            128..958
                     /locus_tag="U6309_002764"
                     /pseudo
     CDS             128..958
                     /locus_tag="U6309_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213145.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(948..1760)
                     /locus_tag="U6309_002765"
     CDS             complement(948..1760)
                     /locus_tag="U6309_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5506176.1"
                     /translation="MSMYDKRHFFKTYFSSASEKSILVKLLDKRFHPPSHTANILDLG
                     CHDGVLMKNIVNTYAKRLPEKLVITGVDPSLQAIKEYAKADFPIPVQTNTFAGTAEDY
                     FDVCTDHFDWIFASQCLYWSPDLPKIIKQIHEAGDSSLIVIRGCHGIYEIQSQFRHYI
                     GNHLEKFYTDKEVEMALRTQKILFQKESLSTSIRLPDKDSIELKWLILFFLQMEEESL
                     GDKIFCEVRDWITSRTSGSIHHEVHFFWLGNAIFTGDYNMNFPVKSKKNSMG"
     gene            complement(1796..2728)
                     /gene="legD2"
                     /locus_tag="U6309_002766"
     CDS             complement(1796..2728)
                     /gene="legD2"
                     /locus_tag="U6309_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegD2"
                     /protein_id="HEN5506177.1"
                     /translation="MGNLLNEKKLTIFTSNEIQDNLNRVLKDYEQNGIIGLNGYLNQA
                     NRISLLNELEIIQKDAEQDIVRYWNNKIIHFYSKDPLKPESVDREYVTEPYFQASSNK
                     AHVFYEVIDNMRVVNRIGHGMHLINRYPMMQRTVYDSPVLLDLLKGIGFKKPICHLSV
                     YIPKYPNGIGSEVRPHQESTFAFTEPQSVVVLWVALEDALIENACMYGVLGSNHWPLK
                     WVSKVDRETKTRHFEQVHQLHIPDFMTEREFYTALEVKAGDALLFHGNFVHCSPMNTS
                     KNSRKALSFQFIETYEVDYPETNWLYPPNKVYLY"
     gene            complement(3068..3331)
                     /locus_tag="U6309_002767"
     CDS             complement(3068..3331)
                     /locus_tag="U6309_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506178.1"
                     /translation="MADQPPKSKWHEIKRLSEGDNKVDNNQAPVSTADRIKKLYLDTQ
                     VGSPIDTVEEADAQRNNLGPKDTKIENSSHENDEPNPDIPTPG"
     gene            complement(3502..3726)
                     /locus_tag="U6309_002768"
     CDS             complement(3502..3726)
                     /locus_tag="U6309_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEN5506179.1"
                     /translation="MKHFDFSLRIWPKIIDINGKEKYFFRDYLLSHPEAAEKYQQLKI
                     KLAQEHKYDREQYTDAKSEFINQILKLAQN"
BASE COUNT         1187 a          716 c          631 g         1219 t
ORIGIN      
        1 caaatagtaa gacaagaaat ctcaggaaaa accctatgta agacacacag ccagctatgg
       61 tttgctctgt tttttgagtc aaaatagagt ctgtttgcag ataataaatc gagaaggaga
      121 ttgaacgatg gaccttggct ttccaacttt tatatatgga accgcctgga aagaaaagcg
      181 tacaagcgaa ctggtcgagc ttgcattaac taccggattt cggggtatag atactgcaaa
      241 ccaaagaaaa cactattatg aagaaggtgt tggatatgcc ttgcaacaat actataaaaa
      301 atcaggtatc ttgcgtgatg atattttttt gcaatccaag ttcacctatg ctcatggcca
      361 ggatcatcgc aaaccttacg atgatacagc aagcctgact attcaagtcc agcaatcttt
      421 tgcaagcagc cttgaacatt taaatactga ttatttaaat tcctatattt tgcatggccc
      481 ttattaacaa tatcattttt ctgatgaaga taaagaagtc tggaacaaaa tggaatccct
      541 gcataaagat gggaaagtaa aacacctggg catatccaat gtgagttatg aacagctagt
      601 cgaactcatt tcatttgctt cggtaaaacc caagtttgtt caaaatagat gctttgccag
      661 gctattatgg gataaacaaa ttcgtaacta ttgccaggct catggaatta tttatcaggg
      721 tttttctctg ttaacggcca atcaatatga gttaagacat ccgaaacttg agaatctcag
      781 caaaaagtac gagaaatcga ttgagcaaat catttttaga ttttcacagc agataggaat
      841 gttgccttta actggcacga caaatttgga tcatatgaaa gaagatcttg ccatccatca
      901 gttcaggctg actgatgatg aaatcaattt cattgaaaac attgccttta tcccatagaa
      961 ttttttttcg atttgactgg gaaattcata ttgtaatccc ctgtaaaaat ggcattccct
     1021 aaccaaaaaa aatgaacttc atgatgaatg cttcctgacg ttcgagaggt aatccaatcc
     1081 cgcacttcgc aaaatatttt atcacccaac gattcctctt ccatttgcaa aaaaaacaaa
     1141 atcagccatt tcaattcaat actgtcttta tcaggcaacc ggatagaagt acttagagat
     1201 tctttctgaa ataaaatttt ttgggtcctt aaggccatct caacttcttt atcagtataa
     1261 aatttctcta aatgattccc aatataatgc ctgaattgcg attgaatttc ataaattcca
     1321 tgacatcctc tgataacaat taaagaggaa tctcctgctt catgaatctg cttaattatt
     1381 tttggcaaat caggggacca atacaaacac tgcgatgcaa aaatccaatc aaaatggtca
     1441 gtgcacacat caaaataatc ttctgcggtt ccagcaaaag tattcgtttg gactggaatt
     1501 ggaaaatctg cttttgcata ttctttaatt gcttgcaaac taggatccac tccggtaata
     1561 actaactttt ccggtaaacg ctttgcgtaa gtattcacta tatttttcat caatacgcca
     1621 tcatggcaac ccaaatccaa tatgttcgca gtatgagagg gaggatgaaa tcgtttatcc
     1681 aacagcttta ccaggattga cttctcgctt gcactagaaa aataggtttt aaaaaaatgg
     1741 cgcttatcat acatagacat gtagtgattc ctcaattgct tatcaaattt aatttttaat
     1801 ataaatacac tttgttagga gggtataacc aattagtttc cggataatcg acttcatacg
     1861 tttcaataaa ctgaaacgac aatgcttttc ttgagttttt agacgtattc atcgggctgc
     1921 agtgcacaaa attaccgtga aataacaaag catctccggc tttcacttcc aaggcagtat
     1981 aaaattcgcg ctctgtcata aaatcaggaa tatgtaactg atgaacttgc tcaaagtgac
     2041 gcgtttttgt ctctcggtcc acttttgata cccatttcaa tggccaatga ttacttccta
     2101 aaaccccata catacaggca ttctcaatta acgcgtcttc gagggcgacc caaagaacaa
     2161 caacactttg tggttcagta aaggcaaatg ttgactcctg atgaggtctt acctcactgc
     2221 ctattccatt aggatatttg ggaatataga cactgagatg gcagattggc ttcttaaatc
     2281 caatcccttt tagcaaatcc agcagtacag gactatcata aactgttcgc tgcatcatcg
     2341 gatacctatt gatgagatgc ataccatggc caattcgatt gactacccgc atattatcaa
     2401 tcacttcata aaatacatgt gccttattac ttgaagcttg aaaataaggt tctgtgacat
     2461 attctctatc cactgactca ggtttcaaag gatctttgga ataaaaatga atgattttat
     2521 tattccaata cctgacaata tcctgctcag catccttttg tattatctca agttcattaa
     2581 gcaagcttat tcggtttgcc tgattgagat atccattaag tccaataatg ccattttgtt
     2641 catagtcttt cagaacccgg ttcaaattat cttggatttc attagatgta aaaattgtta
     2701 attttttttc attcaataaa ttgcccataa caactccaaa ccaatgtcat ggctaaattt
     2761 gattgtaaaa tgctaggaaa taaattagcg tctgcttgca ggcttaatat atatggggaa
     2821 tctggtttat ctgacacagt tatgacctct ttaataaaaa ataaagaagc catcaccaaa
     2881 aacctaatcg cgtgttcaat caggatgact aaaagaatac tgaacactaa ggattttctt
     2941 gtaggaaaat ttacaagtat ttattatggg tgtcaacaca aaaccaacac atgtgtttgc
     3001 tgaacccgaa tggtatagtt aaacagcaaa aagcatttta tttaaaaaat gtatttaatt
     3061 tctgccatca tccgggggtt ggtatatcag ggtttggctc atcattctca tgggatgaat
     3121 tttctatttt agtatcttta ggccctaaat tatttcgctg ggcatccgct tcttcaacag
     3181 tatcaattgg actgcctacc tgtgtgtcca gataaagctt tttaattcta tccgccgttg
     3241 acactggtgc ttgattgtta tctactttat tatcaccttc tgatagacgt ttaatttcat
     3301 gccatttgga ttttggaggt tgatctgcca ttgcgttcct caactagagt aatttagtac
     3361 aattatagat cacaatgttt gtagatcaca acattaagat tatgacttct aaatacgttg
     3421 atgtatcgtt tagatctaac cctgggccaa aaaatccaag ttcgttacac ccatttatcc
     3481 ttgtcttccc atgataattt attaattttg agctaatttt aaaatttggt tgataaattc
     3541 actttttgca tcagtgtatt gctccctatc atatttgtgt tcctgagcca gtttgatttt
     3601 tagttgctga tatttttctg ctgcttctgg atgagagagt aaatagtctc taaaaaaata
     3661 tttttccttt ccattgatat ctatgatttt tggccagatt cttaaggaaa aatcaaaatg
     3721 tttcatttgg attagtttag agtcaccgga tat
//
LOCUS       DASLOE010000226         3730 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.227, whole genome shotgun sequence.
ACCESSION   DASLOE010000226 DASLOE010000000
VERSION     DASLOE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.227"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..459)
                     /locus_tag="U6309_002769"
     CDS             complement(<1..459)
                     /locus_tag="U6309_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HEN5506180.1"
                     /translation="MRLQLMKITGFTLLETMLALALLLGLLLLGSWSFFSLIQNNERE
                     SLVNSIKTAIQYSKIQAIHLGHPIYLLPFGSNENWSRGMVLAILNRKSNKTELIYQWQ
                     WSSNSWNINWKGVDSNHRIIISNIPNRAMSNGKFILNNRRTNEKIVVTLNR"
     gene            complement(465..1244)
                     /locus_tag="U6309_002770"
     CDS             complement(465..1244)
                     /locus_tag="U6309_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN5506181.1"
                     /translation="MMTLITGATRGIGLALAYEFASHGHDLILIGRDSEQLEHISKQL
                     NTQYQVNVEYKVHDLSKPGSAFKLYTDLKEQGIEVNCLVNNAGIGYMGSFVEMGMNKL
                     DDLMNINMISLSELTLCFLHDFVARNEGSILQVSSTAAFQPGPFMAAYYASKAYVATL
                     SHAIAYELKDTKVSMSILCPGATKTNFFHASGMENSMLERGYIGMMNPEKVAKIAYKG
                     LKKGKLYIIPGLRNKILAYAAAISPKAIALRIASYLHHKIL"
     gene            complement(1189..3504)
                     /locus_tag="U6309_002771"
     CDS             complement(1189..3504)
                     /locus_tag="U6309_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN5506182.1"
                     /translation="MLKNWLFNNKSEHWKITDLGLSVNYQLTGIEMCQGKQGILIGFN
                     QFLVPPSGTKYRDPNDNQSEPLGNVKRVFITDKLVDELVGLNYKNLNYESVCKSPHPQ
                     SIDVTDPEARHKDMYDYRKMQRFNGVRPWYIRLWRWISKLFFGDTYDAKKTSTADDNL
                     ELLYKTKTVKIYQRSKSKEILVTEKRPDITNLVFCGGGAKIFAHVGVWKALNEAGMKP
                     TKFAGSSAGAIMSLMCYLGYTADEISELFKHFKQEHLVQFDIDRNGLSDTHSLKTALD
                     YAIANKVRQIVNKYKIPYPKGKITFSTLASLREQFPDCGIGKELIVTATNKRSGKTIY
                     LSANRYPLLEVSEAVKISASFPVLYRDTLLDGEEHNDGGILSNFPTEVFFDDHSTLLE
                     SEFGNNLKVLAVQFDNGIERNVIDRIMDKVYRENFVLNWIYSLLTGVSDPASGWERDR
                     LKLRQYALQTIIPETGKVSTTGFNVERQSQEELIQNGYQSTLDYLNVRYTRKNNSYIN
                     KEIMYSTFNSLGDLLAYCCYRGDEQWFNVVNNLIVQSTLPNRTALMKQSLELRKLYFN
                     TSLPSSPEKNRTACNESNTLTFFGNAVCQHHNHSQELENGNHDVLLALYPIFLKLTTD
                     LLINKIDKNLLDRARHSLNIHSPFDCLEHFNKISGNAHVMIHIIVKLIKELKERSCQQ
                     IYDVLKEVRHLLYSDINLMKANYFGKWDLSLPQCIRILNLLKQEKHDDVSRQLDCLRN
                     QMEPVQTVKGGVHHDDLNNGGHERYRLGLGV"
BASE COUNT         1183 a          732 c          565 g         1250 t
ORIGIN      
        1 cctatttaaa gtaaccacaa ttttttcatt ggttcgtcta ttattcaaaa taaatttacc
       61 attgctcatc gcacgattcg gaatattgga tataataatt ctatggtttg aatctacgcc
      121 tttccaatta atattccagg aattagaact ccattgccat tggtaaataa gctcggtttt
      181 attggatttt cgattaagta tagcgagcac catccccctg gaccaatttt cattagaacc
      241 aaaaggaaga agataaatcg gatgtcccaa atgaatagcc tgtattttag agtattgaat
      301 ggctgttttt atactattaa ctaatgattc tctctcattg ttttgtatta gtgaaaaaaa
      361 tgaccatgaa cctaaaagca ataatcctaa aagcaaagct aatgcaagca tcgtttcaag
      421 tagagtaaat cctgttattt tcatcaattg aagccgcata tcatctacaa aattttatga
      481 tgaagataag atgcgatcct aagagcaatc gctttgggtg atatagctgc ggcataggct
      541 aaaattttat ttcttagccc cggaataata tacaactttc ctttttttaa tcctttatag
      601 gcaattttag cgaccttttc agggttcatc attccaatat atcccctctc caacatagaa
      661 ttttccatac cagaagcatg gaaaaaattg gtttttgttg ctccaggaca aagaatagac
      721 atagatacct tagtatcttt aagctcataa gcaatagcat gagaaagcgt tgctacatat
      781 gccttactcg catagtaggc tgccataaaa ggacccggtt gaaatgctgc tgttgaagaa
      841 acctgtaaaa tactaccttc gtttcgagct acaaaatcat gaagaaaaca tagtgttaat
      901 tcacttaaag aaatcatgtt tatgttcatt aaatcatcga gcttgttcat gcccatttca
      961 acaaaagaac ccatatagcc tatgcccgca ttattaacca ggcagttgac ttctataccc
     1021 tgctccttta aatcagtata caacttaaat gcgcttccag gtttacttaa gtcatgtact
     1081 ttatattcaa cattaacttg atattgtgta tttaattgct ttgaaatgtg ctccagctgt
     1141 tcagagtctc taccaataag tattaaatca tggccatgtg atgcaaactc atacgccaag
     1201 gccaagccta tacctctcgt ggcccccgtt attaaggtca tcatgatgca ctcctccttt
     1261 tactgtttgc acaggctcca tttggttacg taggcaatcc aattgccgag atacatcgtc
     1321 atgcttttcc tgttttaaca aattcaagat ccgtatacat tgagggagac ttaaatccca
     1381 tttgccaaaa taatttgctt tcataagatt aatatcgcta tacaataaat gcctaacctc
     1441 ttttaataca tcataaattt gttggcatga tctttctttg agctctttaa ttaatttaac
     1501 gatgatatga atcataacat gagcatttcc tgaaatttta ttaaaatgct caagacaatc
     1561 aaagggagag tgtatattta gtgaatgacg tgcacggtct aataggttct tatcaatttt
     1621 atttattaat aaatcagtag ttagttttaa aaaaattgga tagagcgcca gtaagacatc
     1681 atggtttcca ttttcaagct cctggctgtg attatgatgc tgacaaaccg cattaccaaa
     1741 aaaagttaaa gtattgcttt cattacaagc tgttctattc ttttcaggac tagaaggtag
     1801 agacgtatta aaataaagtt ttctaagttc aagggattgc ttcattaaag cagtacgatt
     1861 aggtaaggta gattgcacaa ttagattatt aacaacatta aaccattgtt catctcccct
     1921 gtaacaacag taagcaagca aatctcccaa agaattgaaa gtagaataca ttatttcctt
     1981 gttaatatag ctgttgtttt ttctagtata tcttacatta agataatcca gagttgattg
     2041 ataaccattt tggatcaatt cctcctggct ctgcctctct acattaaatc ctgttgttga
     2101 tactttccct gtttcaggaa ttatagtttg aagagcatat tgtcttaatt taaggcggtc
     2161 tctttcccat ccacttgcag gatcactcac tccagtcagt aagctataaa tccagttaag
     2221 aacgaaattc tctcgataaa ctttgtccat tattctatca ataacatttc tttcaatccc
     2281 attatcaaat tgaactgcca atacttttaa attattacca aattcagact ccaacagcgt
     2341 tgagtggtca tcaaaaaaaa cttcggttgg aaaattactt aatattccac catcattatg
     2401 ctcttcacca tccagcagag tatctctata caaaactgga aagctcgcag aaattttaac
     2461 tgcttcactg acctccaata atgggtaacg atttgctgat aaataaatag tttttcctga
     2521 gcgtttattt gttgctgtaa caattaattc tttgcctatt ccacaatctg gaaattgctc
     2581 tcttagagaa gctaaagtac taaaagttat ttttccctta ggatatggaa ttttatattt
     2641 atttactatt tgccttactt tatttgcgat agcataatcc agcgccgtct ttaaggaatg
     2701 ggtatccgat aatccatttc tgtctatatc aaattgaact aaatgctctt gtttgaaatg
     2761 cttgaaaagt tcactaattt catcagcagt ataacctagg tagcacatca aagacatgat
     2821 tgcaccagca gagcttcctg caaatttggt tggcttcatt cctgcttcat tgagagcctt
     2881 ccaaaccccc acgtgagcaa aaatttttgc cccacctcca caaaaaacca aattagtaat
     2941 atcaggtcgc ttctctgtga ccaagatctc ttttgatttg gagcgctgat aaattttgac
     3001 agttttagtt ttataaagta attccaaatt gtcgtctgca gtgcttgttt ttttagcgtc
     3061 gtatgtatcg ccaaaaaata gtttagatat ccatctccaa agtctgatat accaaggcct
     3121 cacaccattg aaacgttgca tttttctata atcatacatg tctttatgac gagcttctgg
     3181 atcagttaca tcaatagatt gcggatgagg gcttttacag acagactcat aattaagatt
     3241 tttataattg agtccaacaa gttcatcaac cagtttatca gtaatgaata ctcttttaac
     3301 attacctaat ggctcacttt ggttatcatt aggatctctg tattttgtac ctgaaggtgg
     3361 tactaaaaac tggttaaatc caattaagat accctgcttt ccttgacaca tttcaatacc
     3421 agtcaactga taattgacag aaagtcccaa atccgttatt ttccaatgct cagatttatt
     3481 attaaataac cagtttttta agatacccca ccaacttaaa gcaacctccg cagtgaaacc
     3541 tcgatattca ttcacgtaaa cgccacttaa aaaaccatgc tctttatctt tttgatgagg
     3601 ctcaagcaac cattcaataa ataaagctct ttttgaatcg ctcaatcgag tgaaatcaaa
     3661 aatcatacgt tcatcatcaa ataaataatt tccaagtgca atcttgttat ctggaggtaa
     3721 tccattaatt
//
LOCUS       DASLOE010000227         3659 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.228, whole genome shotgun sequence.
ACCESSION   DASLOE010000227 DASLOE010000000
VERSION     DASLOE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.228"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1529
                     /gene="ppk1"
                     /locus_tag="U6309_002772"
     CDS             <1..1529
                     /gene="ppk1"
                     /locus_tag="U6309_002772"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEN5506183.1"
                     /translation="HAPRSIPRMIHLPSELCGDAHYFVYLSSIIKTHINSLFPGMEIS
                     GCYSFRLTRNSDLFLREEEIDDLAKAVQREIFSRHYGHVVRLEIENKCPEKIVDFLLQ
                     KYHLRHEDTYYCDGPVNLQRYMSAINSIDRPDLNYPTFTPQYPKFSKSQRNLFNVLDE
                     QDILLHHPYQAFDLVIDFVRQAASDPNVLAINQTLYRTHSESVMVDALVNAAHSGKEV
                     TAVIELRARFDEESNLKLANKLHAAGILVLYGVVGYKTHAKMTLVVRRTHGKLKRYVH
                     LSTGNYHEQTAKRYTDLGLLTSEPTITSDAQLIFQQLTGLGKVVKLKALSHSPFTLQK
                     TLLQFIEQCSTAATEGIDTEIILKVNGLTDKVMIQALYKASQAGVKINLLVRGVCCLK
                     PGLPGVSENIRVLSFIGRFLEHHRVFYFRINEEEHYFCSSADLMERNLYNRIEIMFPI
                     YDEGCKKRIKNEIIKNYLKDNNNVWEMQSDGTYKHLMQNGGCAQEKLIALYEEEDKSI
                     "
     gene            complement(1598..2578)
                     /gene="epmB"
                     /locus_tag="U6309_002773"
     CDS             complement(1598..2578)
                     /gene="epmB"
                     /locus_tag="U6309_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HEN5506184.1"
                     /translation="MRDTSLSWQKILAQGFTSTTDLLDFLELPRSEGNLFAEKQFPSR
                     IPLGFAKRMQKGNPKDPLLLQVLAKEDELTEADDYVIDPLSESNTLIKGMLHKYHGRV
                     LLTLTGVCAVNCRYCFRRHFPYQANNPGRRGWKEVCAYIANDPSITEVILSGGDPLLA
                     ANLVLEELLQSLEEISHVHTLRIHTRIPIVLPERIDKGLLDLLTNTRFKKVIVVHCNH
                     PQELDESVLRACSDLKKAACYLLNQSVLLAGVNDDAGILSRLSHALFDYGIMPYYLHL
                     LDKVKGSAHFDMPLPRAQSIYHQLQSLVPGYLLPRLVREEPGRSSKTMLI"
     gene            2650..3219
                     /gene="efp"
                     /locus_tag="U6309_002774"
     CDS             2650..3219
                     /gene="efp"
                     /locus_tag="U6309_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEN5506185.1"
                     /translation="MAVYSTNEFKNGLKVMVDDAPCSILDCEFVKPGKGQAFTRIKIR
                     NLKTGRVVERTFKSGDTLPSADVADVEMQYLYNDGEYWHFMVPDTFEQYAVTENVLAD
                     AAQWLKEQDVCVVTLWNNEPIQVTPPNFVILAITETDPGLKGDTSGGGGKPATLETGA
                     VVRVPLFVQTGELIKVDTRKGEYVSRAKE"
     gene            complement(3273..3503)
                     /locus_tag="U6309_002775"
     CDS             complement(3273..3503)
                     /locus_tag="U6309_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506186.1"
                     /translation="MNKLIVAVTAGASLLLLTGCAVDGVAVDDVSYDSGYSNDYVYSV
                     GYYSNRPYWGNNYYSSPSFVGPYGYWGGDYDF"
BASE COUNT         1198 a          728 c          710 g         1023 t
ORIGIN      
        1 tacatgctcc ccgctcgatt ccacgcatga tacatttgcc ttctgagctt tgtggagatg
       61 cccattactt tgtttatttg tcttccatta ttaaaacgca tattaatagc ttatttccag
      121 gtatggaaat aagcggatgt tattcatttc ggttgacgcg aaatagtgat ttatttttaa
      181 gagaagaaga aattgacgat ttggctaaag cggtgcaaag agagatattt tcccgtcatt
      241 atggccatgt cgtgagattg gaaattgaaa acaaatgccc tgaaaaaatt gttgacttcc
      301 tcctgcagaa atatcatttg cgacatgaag acacctatta ttgtgatggg cccgttaatt
      361 tacaacgcta tatgagtgct attaacagca tcgatagacc tgatctgaat tatcccactt
      421 ttaccccaca gtatcccaag ttctccaaat cacaacgtaa tttatttaat gttctcgacg
      481 aacaagacat tttattacat catccctatc aagcatttga tttagtcatt gattttgtaa
      541 gacaagctgc ttcagaccct aatgtactgg caattaatca aacgctttac agaacccact
      601 ctgaatcagt gatggtcgat gcgctggtta acgccgccca ttcagggaaa gaagtaaccg
      661 ctgtcattga gctaagagcc cgttttgacg aggaatccaa cttaaaattg gctaacaaac
      721 tccatgctgc ggggatcctg gtactttacg gagtagtagg ctataaaact cacgcgaaaa
      781 tgacgctggt agttcgaaga acgcatggta aattaaaacg atatgtgcac ttaagcacag
      841 gaaactatca tgagcaaaca gccaagagat acactgattt gggattatta accagtgaac
      901 ccactattac ttctgacgcc cagctcattt ttcaacaatt aaccggtctt ggaaaggttg
      961 ttaagttaaa agccttgagc cattcaccat ttaccttaca aaaaactctt ttacaattca
     1021 ttgaacaatg cagtactgct gctacggaag gcattgatac tgaaatcatc ctgaaagtga
     1081 atggattaac tgataaagtg atgattcaag cattatataa ggcatcacag gcaggcgtta
     1141 aaattaatct tcttgtacgt ggggtatgtt gtttaaaacc aggattgcct ggtgtgtctg
     1201 agaacatcag agtcttgtct tttattggca gattcctcga acatcacaga gtgttttatt
     1261 ttcgcatcaa cgaagaagaa cactattttt gctccagtgc cgatctgatg gaaagaaatt
     1321 tatacaatcg tattgaaatc atgttcccca tttatgatga gggatgtaaa aaaagaataa
     1381 aaaacgaaat cattaaaaac tatcttaaag ataataacaa tgtttgggaa atgcaaagtg
     1441 acgggactta caagcacctc atgcaaaacg gcggctgtgc tcaggaaaag ctgattgcac
     1501 tttatgagga agaggacaaa tcgatttagt cagaaaaata acagtagaaa catggctatt
     1561 gaataaaacc atcaatagcc aggacatatc aagctcttta aatcagcatg gttttactgg
     1621 atcttcccgg ctcttctcgt accagacgag gcaataaata tcctggcaca agggattgta
     1681 attgatggta aattgactga gccctaggaa gcggcatatc gaaatgtgcc gaacccttta
     1741 ctttatcgag caaatgcaag tagtaaggca tgatcccgta atcaaaaaga gcatgactta
     1801 gcctggataa aatccctgca tcatcattaa ccccggctaa taaaacagat tgattcagta
     1861 aataacatgc tgctttcttc aaatcagagc acgcccgcaa tacactctca tccaactctt
     1921 gcggatgatt acaatgaaca acaatcactt tcttaaatcg cgtatttgtt aataaatcaa
     1981 gtaatccttt gtcaatacgc tccggtaaaa caataggaat tcttgtatga attcttaaag
     2041 tatgtacatg agaaatttct tccaaagatt ggagtaattc ttccaaaacc aaattggcag
     2101 cgagcaatgg atcgccacca cttaaaataa cttcagtaat actgggatca tttgcgatgt
     2161 aggcacacac ctctttccat ccccggcggc ccggattatt cgcttgataa ggaaaatgtc
     2221 ttctaaagca ataacgacaa ttcacagcgc aaacaccggt taaagttaac aaaacacgcc
     2281 cgtgatattt atgcaacata cctttaataa gggtattact ttcactcaga ggatcgataa
     2341 cataatcatc tgcttcggta agctcatctt cttttgcaag aacctgtaaa agcaagggat
     2401 ctttgggatt ccctttttgc attcgcttag caaaacctaa aggaatacgg cttggaaatt
     2461 gtttttccgc gaataggttt ccttcagatc tcggtaattc caggaaatct aataaatcag
     2521 tcgttgaagt aaatccttgt gccaaaattt tttgccaact caaagaggta tctcgcatta
     2581 aagttacaga cttgataaac tgtgcgaact ttagcgaaaa tacagcaaaa tctcaactat
     2641 ggagcagaaa tggcagtcta tagcacaaat gaatttaaaa acggcttaaa agtaatggtt
     2701 gatgatgctc cgtgcagcat tcttgactgt gaatttgtaa aacctggaaa agggcaagca
     2761 tttacccgta ttaaaattcg caatctaaaa accgggcgcg ttgtggaacg tacctttaaa
     2821 tctggggaca ccttaccatc tgctgatgtg gctgacgtag aaatgcagta tctatataat
     2881 gatggggagt attggcattt tatggttcca gatacgtttg aacaatatgc cgtgactgaa
     2941 aacgtattag ccgatgcagc acaatggtta aaggaacagg acgtttgtgt agttactcta
     3001 tggaacaatg aacctataca ggtgactcca cccaattttg tcattttggc aatcacagaa
     3061 acagatcctg gattaaaagg agacacttct ggcggaggtg gaaagccagc cacgttggaa
     3121 acaggtgccg tggttcgtgt accattgttt gttcaaactg gcgagctgat taaggtagac
     3181 acgcgcaaag gcgaatatgt gtctcgcgct aaagagtaat tatcaacagg aatagccttc
     3241 atttgatttg aaggcttgcc ttctcacgga aactagaaat cgtaatctcc accccaatat
     3301 ccataaggac ctacaaaaga aggtgacgaa tagtagttgt taccccaata gggacgattg
     3361 ctataatatc caaccgaata aacgtagtca ttactataac ctgaatcgta agaaacatca
     3421 tcaacagcca caccatcaac cgcacatcca gttagcagca ataatgatgc accagcagtg
     3481 accgcaacaa ttaacttgtt cataattgct tcctggcaaa atagttgttc tttaattata
     3541 agataatatt attaattgct caaattgctc tttattttaa acagaaagta tcgggattga
     3601 ctttaaccgg atttacgcaa aaccataagg ggcattaaaa aatgttttaa tgagagagc
//
LOCUS       DASLOE010000228         3643 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.229, whole genome shotgun sequence.
ACCESSION   DASLOE010000228 DASLOE010000000
VERSION     DASLOE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.229"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            60..1406
                     /locus_tag="U6309_002776"
     CDS             60..1406
                     /locus_tag="U6309_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEN5506187.1"
                     /translation="MRKWIISLLLLVIIVLCSYPIIGMMALSTVKKNVDNIPKSPFLT
                     WQLKDNQSGWFCSTAVLNLLVDLPAQENKDAQGNIKIQPALHLSMDFPVIVRHGPFIM
                     TNNGLRFGLAWVTTKPETHYGALINYLNQTAVSYSLPSINMQGTNNGDQGKFVFVWQG
                     VSASLKVNPRIDKASGHVTMYGMSTQVQETDVRIGKVKVNFALNRYMDDLWLGDSSIM
                     LSSLSIITNSQPVFDLQNLGIDAWAGVNSNLLDFNLQLSLNKFVMNNNSYGPGQLKIE
                     VKNLDPQAMAKLNHLNYDYPSNQIAILAEMFNLLSKGATLNLNQELDTPQGHLSSSLN
                     LALTRSSFSNPEDLLQNLKGNGQFKAPIKLVEQLLFESIKSKTATPAADMSQSQVTEP
                     LVDADAEAHKQTEELLQKLVSKGILKVEGDYYQASFTLENTKFTVNGQPFDPALLN"
     gene            1596..2168
                     /locus_tag="U6309_002777"
     CDS             1596..2168
                     /locus_tag="U6309_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5506188.1"
                     /translation="MALIRDSKSQVMSVFKFQPLGIKDLNQLYQWFQEPIINHWYARD
                     KSWSLDEITEKYEPRIAGMEHVPSFIVYKDDLPFGFVQFYLITDSLPDGIDGYESLLF
                     EKYNPRDLAGIDLFIAESEGRGRGLGVELINRFIDKFLTRFKAVVVDPDVNNQQAIRC
                     YEKAGFQQTGFSQDPNHLIMIKPLSLITGN"
     gene            2223..3422
                     /locus_tag="U6309_002778"
     CDS             2223..3422
                     /locus_tag="U6309_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEN5506189.1"
                     /translation="MIKSFRVTIFLFLVSGLILARDMHAFPSGLESNQDKLVYFQLEA
                     ETFYSPKPVTIEGQFYLTYEVFLANVGDYLFSLENVEVLNGRKPYKVLFSYNQEELRK
                     MIYSFSGHSNHLTSDLTLRPGERGLLFFMVKFDSLDEIPEKIMHRFHLYSSKTKISEE
                     GVRYSFLAAPAYVKITKPLVIANPLDGKHWMAINGPSNHSIHRRARVTAHGIVYYPER
                     YAIDFMQFGVDGHVYKENPNKNENYYSYGANVYSVADGEVVAVYDGVPDNTPPGRDYP
                     VTLSNMAGNHVIIKIGKDQYALYAHLIPHSIKVTKGELVRKNQLIAKLGNSGNSDAPH
                     LHFHVIDKPSPLIGQGIPYAFEHFSTEEYRFAGDEWESPIVYEGRLTEHHHELVHENT
                     LINFEEK"
BASE COUNT         1089 a          681 c          769 g         1104 t
ORIGIN      
        1 tttacaagca gccttttcta gtctaaatat taattaccaa ggattaatat tggacagcca
       61 tgagaaaatg gattatcagt ttgctgctgt tagtaatcat cgtcttatgc tcatacccta
      121 tcattggcat gatggcctta agcacggtga aaaaaaacgt cgataacata cccaagagtc
      181 ccttcttgac ttggcaatta aaagataatc aaagtggatg gttctgctcg actgccgttt
      241 tgaatttact tgttgatttg ccagcccagg aaaataaaga cgctcagggt aatattaaaa
      301 ttcagccagc acttcatttg agcatggatt ttcctgtgat tgtgcgccat ggccctttca
      361 tcatgaccaa taatggcttg cgttttgggc tagcatgggt caccacgaaa ccggaaactc
      421 attatggagc tttgattaat tacttaaatc aaaccgctgt cagttatagt ttgccctcga
      481 ttaatatgca gggtacgaat aacggtgatc aaggcaaatt tgtttttgtc tggcaaggcg
      541 taagtgcaag cctcaaagtc aatcccagga ttgataaggc ctctggccat gtgactatgt
      601 acggcatgag tactcaggtt caagaaaccg atgtaaggat tgggaaagtc aaggtcaatt
      661 ttgcattgaa tcgctacatg gacgatttat ggcttggtga ttcatctatc atgctgtctt
      721 ctttgtctat catcactaac tcacagccag tgtttgattt acaaaatttg ggtatagatg
      781 catgggctgg agtcaacagc aatttactgg attttaattt gcaattatcc ctgaataagt
      841 ttgttatgaa caataacagc tatggtcctg gccagttaaa aatagaggtt aaaaatctgg
      901 atcctcaagc gatggccaag ctcaatcact tgaactatga ctatccttcc aatcaaatag
      961 cgatcctggc tgaaatgttt aacctgcttt ccaaaggcgc gacactcaat ctgaatcaag
     1021 agctggatac gccacagggg catttgtcca gttcgttgaa cctggctcta accaggagtt
     1081 cattcagcaa tcctgaagat ctcttgcaaa atttgaaagg caatggtcag tttaaagcgc
     1141 caattaaact ggtagaacag ctactatttg aatcaatcaa atcaaaaaca gctacaccag
     1201 cagcagatat gtctcaatct caggttacag agccgctggt tgatgcggat gccgaagccc
     1261 ataaacaaac ggaagaactc ttgcaaaaat tggtgagtaa aggcatttta aaagttgaag
     1321 gcgattacta ccaggcctct tttactctgg aaaacaccaa atttacagtg aatgggcaac
     1381 catttgatcc agcgttattg aattagatta gcggttgagc agtttgctca acctgcactg
     1441 atgagatgat cttctatgtc aatcacaagc aaatccaggc gatcaaaact gtggtagaat
     1501 acccactcag tttttgacta cagtataatg aatcatgatt acccacaaac ctgacctatg
     1561 gaagtcaatt tggcatgcct cggatgtgag tcgtcatggc attgataagg gattcaaaga
     1621 gccaagtcat gagtgttttt aaatttcaac ctctgggcat caaggattta aatcaactgt
     1681 atcaatggtt tcaggagcca atcattaatc attggtatgc gagagacaaa agctggtcac
     1741 tggatgaaat cacagaaaaa tatgaaccaa gaattgcagg tatggagcat gttccaagtt
     1801 tcattgtata taaagatgac ttacctttcg ggttcgtcca gttttatttg atcacagatt
     1861 ctttacccga tggaattgat ggttatgaga gtcttctctt tgaaaaatat aatcccaggg
     1921 atttagctgg aatcgatttg tttattgctg aaagcgaggg acgaggtaga ggattagggg
     1981 tggaactcat taatcgcttt attgataagt ttcttacccg tttcaaagct gttgttgttg
     2041 accccgatgt taacaatcaa caagccattc gttgctacga aaaagcgggc tttcagcaaa
     2101 caggtttcag ccaggatccc aatcatctca tcatgataaa acccttgtca ttgattacag
     2161 ggaattgatt atgagcctat tgaaaataga acgttgtttt ggcaatttac caagggtttt
     2221 aaatgataaa atcatttcgt gtcacaattt ttttatttct tgtaagtggt ttgattctcg
     2281 cacgagacat gcatgctttt ccctctggat tggaatccaa tcaggataaa ttggtttatt
     2341 ttcagttaga agccgaaacg ttttattctc caaaacccgt gactattgaa ggacaatttt
     2401 atctcacgta tgaggttttt cttgccaatg tgggtgatta cctcttctct ttagaaaacg
     2461 ttgaagtatt aaatggcaga aaaccttata aagttctttt ttcctataac caggaagagc
     2521 tgcgaaagat gatatattct tttagtggtc attctaatca tttaacatct gacttgacat
     2581 taagaccggg tgagcgtggt ttgttatttt ttatggtgaa attcgatagt cttgatgaaa
     2641 tccctgaaaa aataatgcat cggtttcatc tttattcatc gaaaaccaaa atatccgaag
     2701 agggtgtcag gtattctttt ctggctgctc ccgcttatgt aaaaattact aagcccctgg
     2761 tgattgccaa ccctttggat ggaaagcatt ggatggcaat taatggccca tccaatcatt
     2821 ccatacaccg ccgcgcaagg gttacagccc atggcattgt gtattacccc gaacgatacg
     2881 ccattgattt catgcagttt ggagttgatg gtcatgtcta taaagaaaat cccaacaaaa
     2941 atgaaaacta ttacagttat ggggccaatg tttactcggt tgcggatggt gaggtagtcg
     3001 cagtttacga tggtgttcct gataatactc cgcctggacg tgattatcct gttacgctga
     3061 gtaatatggc tggcaatcac gtgattatta aaataggaaa agaccaatat gctctctatg
     3121 cccacttaat tccccatagt attaaggtca ctaaggggga gttggttcgt aagaatcaat
     3181 tgattgcaaa attgggtaat tcgggcaatt ctgacgcacc gcatttgcat tttcatgtta
     3241 ttgataaacc atctccttta attggccaag ggatacccta tgcatttgaa catttttcta
     3301 cagaagaata tcgatttgcc ggcgatgagt gggaatcccc tattgtctat gaggggagat
     3361 taacagaaca tcatcatgag ctggttcatg aaaatacttt gataaatttt gaagaaaaat
     3421 aatcgaatat tagacgataa gaataaggcg atataagaag acgcttttat gcacgaaagt
     3481 ttaaatggat ccttggcaaa ttgatgctca taacatggaa tggctcatag tctataatat
     3541 gaggtaacgt gagttatgga taaggcaggt cgttgtcttg aatcgttcgg gctgaggagg
     3601 catttatgcc gtctcgaagc cttgtatgga atttaatgtt tct
//
LOCUS       DASLOE010000229         3623 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.230, whole genome shotgun sequence.
ACCESSION   DASLOE010000229 DASLOE010000000
VERSION     DASLOE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.230"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            42..1850
                     /gene="lem10"
                     /locus_tag="U6309_002779"
     CDS             42..1850
                     /gene="lem10"
                     /locus_tag="U6309_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem10"
                     /protein_id="HEN5506190.1"
                     /translation="MVTKIIWVSNNGKPNLKIEFVSEEEKSNFFKEVKKKASELGLDF
                     PLVQGPGNSLVMQASNYPINPCGCYISPGGKLAINFGKVELSHFILPKVGVKTEHAEI
                     FKEHNTIFFHKHKLPGVNSELTFIPTGTPVIVPVTKPATTSPTFFNPSKSGDSSISIS
                     AIGNVDSTTIRITFQNQTEREFFLNKISDKAKSLGVNISTHPFEIKEPNMVLIKPSKY
                     PDNKLGCYISKNKEIAINFGKTEFRDFVLSNLGISSHLGTCPTKNETGGDTFYFHQEN
                     LSLNGPALSVNTKITKEIEKNDYLLSANGAKAIDFVWSNFLKHPYNPKLKQPDANVKA
                     AWEKHTDWEHWSNMGQDWSLNTPSGLVPRPNHGIAHTLRVAQLVPVIAEFLKAYSGDP
                     KFQKLTQKEIQKAQYMILFSVIGRENDMSWTDANHYQQAFKEAYNGQHSKHIYATFKE
                     NAQKGFLNHVVTNKSSLIPSLFSDESELQYWAEALDTGKPGISSASGILMAIAHDLDL
                     MRCYDKGKFNNLKMKDLVTRLGGNEEAARKLADYAHDLIVATGDRCMGYGVSQDYNFS
                     LFGQCSLDPNECLKKLQSVPKPETTPTASLTNHHGI"
     gene            2070..>3623
                     /locus_tag="U6309_002780"
     CDS             2070..>3623
                     /locus_tag="U6309_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3458 domain-containing protein"
                     /protein_id="HEN5506191.1"
                     /translation="MKIDQSKVKKLNDYKVLDYFVIHVDLQIDLSKKPVESKARLTVV
                     PNPNIDSHSNDLILDGENMTLVSIQMNDNLLKENEYELTKDALVIKNIPQNTPFTIEM
                     ASLLGENTDLFGLYETEGVALVKAESEGLRRVLYLPDRPDNLATYKTTIIANQQDYPV
                     LLSNGVLIEKKELPLGLHSVTWLDDVPKPSYLFALVAGNLQRSVTYYQTKSGRELPIE
                     FYVPPSATAKCDFAKEVLKEAMAWDERTFNLECALRQHMVAGVDKYASGASEPTGLNL
                     FNTENLFATPETKTDLGILRVLEVVAHEFFHYWSGDRVTIRDWFNLPLKEGLTTFRAA
                     MFREELFGTDLIRLLDGKNLDERAPRQSAYTAVRSLYTAAAYEKSADIFRMMMLFIGK
                     KTFIQGVAKFFKDNDGSAVTLEDFIDSLSNSSGKDLRSFLSWFTESGIPELIVTDEFN
                     PDAKQYLLKIKIANGKNRPVPFLMGLLDSSGKEIVADTLLMVDQEEIEFQFENIQTRP
                     IPSLLRSFSA"
BASE COUNT         1204 a          594 c          706 g         1119 t
ORIGIN      
        1 ctataattcc aaaatattcc tgggaatagc agaggccaat tatggttacg aaaataattt
       61 gggtttctaa taatggtaaa cctaatttaa aaattgaatt tgttagtgag gaagaaaaaa
      121 gtaatttttt caaggaggta aagaaaaaag caagcgaatt aggattagac tttccactag
      181 tgcaaggccc tgggaattct cttgtgatgc aggcttcaaa ttacccaatt aatccttgtg
      241 gttgttatat atccccaggg gggaaattag ctattaattt tggaaaagtt gagttgagtc
      301 attttatatt acctaaagta ggcgtgaaaa cagaacatgc tgagattttc aaggaacaca
      361 atactatttt cttccataaa cataagcttc caggtgtgaa ttctgaatta actttcatac
      421 cgactggtac acctgttatt gttcccgtaa caaaaccggc gactacttct ccaacatttt
      481 ttaatccttc aaagtcaggg gattcttcga tttctattag tgcgatagga aacgttgata
      541 gtacgacgat aagaattact tttcaaaatc aaacggagcg agagtttttt ctgaataaaa
      601 tatcagataa agcaaaaagt ttaggcgtca atatctcaac tcatcctttt gaaatcaaag
      661 aacctaatat ggtacttatt aaaccttcta aatacccgga caataaatta ggttgctata
      721 tttcaaagaa taaggaaatt gcaattaatt ttggaaagac agagtttaga gactttgtgt
      781 tgtctaatct tggaataagc tctcatttag gaacctgtcc aacaaaaaat gaaacaggag
      841 gtgatacttt ctattttcat caggagaatc tttctttaaa tggccctgca ttatcagtaa
      901 ataccaaaat aactaaagaa atagagaaaa atgattatct actatctgct aatggggcta
      961 aagccattga ttttgtttgg agtaattttt taaaacatcc ttataatcct aaactgaagc
     1021 agccagatgc taatgtaaaa gcagcttggg aaaagcatac tgattgggag cattggtcca
     1081 atatgggaca ggattggtca ttaaatactc cctcaggttt agtccccagg ccaaatcatg
     1141 gaattgctca tacgttacga gtcgcacagc ttgtaccagt cattgctgaa tttttgaaag
     1201 catattcagg tgatccaaaa ttccaaaaat taacgcaaaa agaaatacaa aaagcacaat
     1261 acatgatatt attttcagta attgggcgtg aaaacgatat gagttggaca gatgccaatc
     1321 attatcagca ggcatttaag gaagcataca atggacagca ttccaagcac atctatgcta
     1381 ctttcaaaga aaatgctcaa aaagggtttt taaatcatgt agttaccaat aaatcgtcat
     1441 taattccttc gctattttct gatgaaagtg aacttcaata ttgggctgaa gctctggata
     1501 cgggtaaacc aggaatttca tcggcctctg gtatacttat ggctattgct catgatctgg
     1561 acttaatgag atgttatgat aagggaaaat ttaacaatct gaagatgaaa gatttagtta
     1621 ccaggcttgg aggaaatgaa gaggcagcaa gaaaactagc tgattatgct catgatctga
     1681 ttgttgcgac aggagatcga tgcatgggtt atggggtgtc tcaggattat aatttctctc
     1741 tttttggtca atgtagcctt gatcccaatg agtgtctaaa gaaacttcag agtgtcccta
     1801 aaccagaaac gactccaacg gcttctttga ccaatcatca tggtatttga gttttaactc
     1861 tctttttaaa tcatactgct ctttaatcaa gtatcctttg agtgcaaagg atactatata
     1921 tcaccataac aaaaacacta acttgattga aattactatc ttgatcttat atcgtgattt
     1981 cactgagctg aaaacagata aatggtttgt aatcagttgc aatgaggaat attaattctt
     2041 agttttttgt ggtaaatcag ggagtgttca tgaaaataga tcaatctaaa gtaaagaaat
     2101 taaatgatta taaggttctc gattattttg taatccatgt tgacttgcaa atcgatctca
     2161 gtaaaaagcc agtagaatcc aaagcccgac ttacagtagt gcccaaccca aatattgatt
     2221 cacattcaaa tgatttgata ttggatggtg agaatatgac attagtgtca atacaaatga
     2281 acgataattt attgaaggaa aacgaatatg agctaactaa agatgcttta gtaatcaaaa
     2341 atatacctca aaataccccc tttactatag aaatggcttc tctgctcggt gaaaataccg
     2401 atttgtttgg attatatgaa acagagggag tagccttagt taaggcagag tctgaaggat
     2461 tgcgccgagt attatatttg ccggacaggc ctgataatct cgcgacttat aaaactacta
     2521 tcatcgcgaa tcagcaagat tatcctgttt tactttcaaa tggtgtgcta attgagaaaa
     2581 aggaacttcc tttagggctg cattctgtaa cctggctgga tgatgtccca aagccaagtt
     2641 atttatttgc tctggtggct ggtaatttac agcgatcagt cacttattat caaacaaaat
     2701 ctggccgtga attaccgatc gaattctatg ttccaccatc agctacagca aaatgtgatt
     2761 ttgcaaaaga agtattgaaa gaagcaatgg catgggatga acgtactttt aatttagaat
     2821 gtgctttgag acaacatatg gtagccggag ttgataaata cgcatcaggt gcttccgaac
     2881 caactggctt aaatttattt aatacagaaa atttatttgc aactcctgaa actaaaaccg
     2941 atttaggtat tttgcgcgtg ttggaagtgg ttgcccatga gttttttcat tattggtcag
     3001 gagatcgggt cacaataaga gattggttca atttgccatt aaaggaaggt cttactacgt
     3061 ttagagctgc tatgttcaga gaggagctgt ttggtacgga tttgataaga ttacttgatg
     3121 gcaagaattt ggatgaaaga gcaccacgac aaagtgccta tacagcagtt agaagcctat
     3181 atactgctgc agcctatgaa aaaagtgctg atatttttcg tatgatgatg ttgtttatag
     3241 gtaaaaaaac atttattcaa ggggtagcca agttttttaa ggataatgat ggaagcgcag
     3301 ttactctgga ggattttatt gattcactta gcaattcatc tggtaaagat cttcgttcct
     3361 ttttatcctg gtttactgaa tctggaattc ctgaattgat agtaacggat gaatttaatc
     3421 cagatgcaaa gcaatatctt ttaaaaatta aaatagccaa cgggaaaaac cgccccgttc
     3481 ctttcctgat ggggttgctt gatagctcag gaaaagaaat tgtagctgat acattattaa
     3541 tggttgatca agaggaaatc gagtttcaat ttgaaaatat ccagacccgc cctattccat
     3601 cgttattacg tagtttttct gca
//
LOCUS       DASLOE010000230         3581 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.231, whole genome shotgun sequence.
ACCESSION   DASLOE010000230 DASLOE010000000
VERSION     DASLOE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.231"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..3538)
                     /gene="sdhA"
                     /locus_tag="U6309_002781"
     CDS             complement(<1..3538)
                     /gene="sdhA"
                     /locus_tag="U6309_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector SdhA"
                     /protein_id="HEN5506192.1"
                     /translation="MISEKIKLLESFKQNQFSKLIEKINSKILATLKSGHFNPRLAPY
                     LEKMNKDGVPFTDFDLDPENISQIKKLINALYHARLAFLDLENIDIGNIKRTIPDLKL
                     LYSNTIHQAYQAGYLITNLDVDLQEMFKEEIDLILPVIGKLQAFAGEHQECTKRLAES
                     LKDFPISYKAGEVTGIALEQMQPRTGDWDYQFLTQFSAVLPGYIEKLTQYIQQYSSQI
                     KEKEPTLNNEKLEELQNASLKLLNDLEHLKGNDFFISLKVLKYIHIIRNIITLATSSV
                     EQMGHFSDSSQDVIRDNLAQLKYVVLPTLFGLVDKIEDNAMLKPGTLSIPLMEKIKPL
                     YQILIYYASKPVNFKEKGEELLSIEDSRFLALRLERTYQRIDEANKALFKIQKAQEAL
                     DNFYKILDTPPYNKQPIHQLPKEIKNQLITYYKIISPYMMDVDPDLNILLIDSFQGPE
                     SWSSYLTKPWRWLRGTHPADHASFVLAQKEALQTLISKKKATQQFHIELNRDLIESVH
                     KQTNLVLFPYSEKTNVFLLDETKALNPANGATDKLKFRPEKGHNILVNPEDLTSEQAL
                     DLYQWYRNKREKFRVASKAYNEFITLLKKQTKTIAETEGRILHLNNLDKETKTHCRNL
                     YNIFQPYFIDGIPPELRASAISFDKFLVHSFSNESSTYDAPAVDLFEKLDEHFQIYFT
                     EIDLQWGKKSNQYLKWAQEKFASENNSTELEHDEKLESRAHHLIKHTNFSKCIHEFRE
                     ELNQVISFFNNAMRAQLKQQPNGIPYPELQDQNKELAQSEQVVAIKRIFNSLYHVEKI
                     VVELEKLDTRSYEMVYVYHLIQAYDHINEIKKLALKLAVDPHFKLIASELLEKTQTLI
                     ATIQEHSDAYQVSPLEVPYNQDIKYNALWYTLNAFYISPKHIRSLRNTNYLTTEELND
                     LHLKAKKATVTVENIIKSSNSYFKLFLQTPAMYSLYREMTNKLNEFISTSHDAVMNNL
                     DQFRSKIFTPMLMEADAWEDKLGLVPGTISGPLKTITDEYYKGLLHPLALHSKAHIRM
                     ICDKTPIDQRIKTTNKKLENATQHLDKIEKNYKHIIKLYEIIKTKGGETTDDELIETY
                     KKALYKLVKLQKRLPQNTDPDPKDYELDTLLNSELKEYEPKLTLIKPLIISSYHHYEG
                     LKATYLMKANTAREKLSYLKELRSTQEQEDLLY"
BASE COUNT          990 a          567 c          690 g         1334 t
ORIGIN      
        1 tataaaggag atcttcttgt tcctgagtcg atcgtaactc tttaagataa cttaattttt
       61 ctctggcagt atttgctttc ataagataag tggcttttaa gccttcataa tgatggtagc
      121 ttgagataat gagaggctta atgagcgtta atttaggttc atactctttc agctctgaat
      181 ttaaaagagt gtctaattca taatctttag gatcaggatc tgtattctgg ggtaatcgct
      241 tttgcaattt taccaatttg tacagagctt ttttataagt ttcgattaat tcatcatcag
      301 tggtttcgcc accttttgtt ttgataattt catatagctt gatgatatgt ttataatttt
      361 tttctatttt atccaaatgt tgtgttgcat tttcgagttt tttattcgtt gtttttattc
      421 tttgatctat tggtgtttta tcacatatca ttcgaatatg tgctttggaa tgcagtgcta
      481 aaggatgtaa caggccttta taatattcat ccgttattgt ttttaatggt cctgagatag
      541 tacctggtac caagcctaat ttatcttccc atgcgtccgc ttccatcagc atgggtgtaa
      601 aaatcttgga gcggaattga tccagattat tcattacggc gtcatgtgat gtgctaataa
      661 attcattcag tttgttcgtc atttctctat agagagaata catggcaggc gtttgtaaga
      721 ataatttgaa ataggagttt gaacttttaa tgatattttc tacagtaacg gttgcttttt
      781 ttgcctttaa gtgtaagtcg ttgagttctt ctgtcgtgag atagttggta tttcttaaac
      841 ttctaatgtg ttttggtgaa atataaaaag cattgagtgt ataccagaga gcattgtatt
      901 taatatcctg gttataaggt acttctaagg gggatacctg atacgcatca ctatgttctt
      961 gtattgttgc aataagagtt tgcgtttttt ccagtaactc gcttgctatg agcttaaaat
     1021 gaggatcgac agcgagtttt agcgctaatt tcttaatttc attaatgtga tcataagctt
     1081 gtattaaatg atagacatag accatttcgt aacttctggt gtccagtttt tccagttcaa
     1141 caacaatctt ttcaacatga taaaggctat taaatatgcg ttttatcgca acaacctgtt
     1201 cactctgggc aagctctttg ttttgatctt gcaattcagg gtaaggtatt ccgttgggtt
     1261 gttgttttaa ttgcgctctc atcgcgttat taaaaaacga aatgacttga tttagctcct
     1321 ctctgaactc atgtatgcat ttagagaaat tggtatgttt aattaaatga tgggctctgc
     1381 tttccagttt ttcatcatgt tcaagttcgg ttgagttatt ttcactggca aatttttcct
     1441 gggcccattt taaatattga ttactctttt taccccattg taaatcaatt tctgtaaaat
     1501 aaatttgaaa atgttcatcc agtttttcaa acaggtcaac tgccggagca tcataggtgg
     1561 atgactcatt agagaaggaa tggaccaaaa atttgtcaaa acttattgcg gaagctctga
     1621 gctcaggagg tatcccatcg ataaaataag gttgaaatat attatataaa ttacggcagt
     1681 gagttttggt ctctttatca agattattta aatggagaat tctgccttcc gtttcagcga
     1741 ttgtcttggt ttgctttttt aataaggtaa taaattcgtt atatgctttg ctagcaactc
     1801 tgaatttttc acgtttgttt ctataccatt gatagaggtc aagagcctgt tcagatgtta
     1861 aatcctcagg attaaccagt atattatgtc ctttctctgg cctgaatttt agtttgtccg
     1921 tagcgccatt agcagggttt agtgcttttg tctcatctag aagaaagaca ttcgtctttt
     1981 cactataagg gaataaaacc agatttgttt gtttgtgaac agattcgata aggtctctat
     2041 tcaattcgat atgaaattgt tgagtggctt tctttttgct aattaaagtt tgtaatgctt
     2101 ccttttgcgc taaaacaaaa ctggcgtgat cggcaggatg tgtgcctctt aaccaacgcc
     2161 acggctttgt taaataagaa gaccaggatt ccgggccctg aaaactatcg atcaacaaaa
     2221 tattgagatc aggatccaca tccatcatat aaggactgat tattttgtaa tatgtaatca
     2281 gttggttttt aatttctttc gggagttgat gtatgggttg cttattatag gggggggtgt
     2341 ctaatatttt gtaaaaatta tccagagctt cttgcgcttt ttgaatttta aaaagggctt
     2401 tgttagcttc atcaattcgt tgatatgttc tttccagtct taaagcgaga aaacgggagt
     2461 cctctatact gagtaactct tctccttttt ctttgaaatt gacgggtttg gatgcgtaat
     2521 aaattaaaat ttggtataga ggtttgattt tttccattaa cggaatggat agtgtgccag
     2581 gcttcagcat ggcattgtct tcaattttat ctactaatcc aaataaagta ggtaatacaa
     2641 cgtattttaa ttgagccaaa ttatcacgaa tgacatcctg ggatgaatcg ctgaaatgtc
     2701 ccatttgttc aacagagctt gtcgccaatg tgatgatatt acggataatg tgaatatatt
     2761 ttaaaacttt aagggatata aaaaagtcat tccctttcag atgttccagg tcattcagta
     2821 actttaatga tgcgttttgt aactcttcca gcttttcgtt atttagggta ggctcttttt
     2881 ctttgatttg cgaagagtat tgttgaatgt attgagttaa tttttctata taaccaggta
     2941 atacggcact gaattgcgtg aggaattggt aatcccaatc tcctgttctc ggttgcattt
     3001 gctctaaggc aatgcctgta acttctcctg ctttatagct tataggaaaa tccttcaaag
     3061 actctgccaa acgcttggtg cattcctggt gttcaccagc aaaagcttgt aattttccaa
     3121 tgacaggaag aataagatca atctcttcct tgaacatttc ctgcaaatca acatccaggt
     3181 tagtgattag ataaccggct tgataggcct ggtgaatcgt gttgctataa agtaatttga
     3241 gatcagggat agttcgtttg atgttcccta tgtcaatatt ctctaaatcc agaaaagcta
     3301 aacgggcatg gtaaagggcg ttaatgagtt tcttgatttg agaaatattt tccgggtcaa
     3361 gatcaaaatc ggtgaatgga accccgtctt tattcatttt ttccaagtag ggggccagtc
     3421 tggggttgaa atgtccactt tttaaagtcg caaggatttt tgaattaatt ttttcaatta
     3481 atttagagaa ttggttttgc ttaaaggatt ctaaaagctt gatcttttct gaaatcattt
     3541 caatattaaa aaaattaact tgtgatatta taaagaaaaa t
//
LOCUS       DASLOE010000231         3575 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.232, whole genome shotgun sequence.
ACCESSION   DASLOE010000231 DASLOE010000000
VERSION     DASLOE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.232"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..484)
                     /locus_tag="U6309_002782"
     CDS             complement(<1..484)
                     /locus_tag="U6309_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506193.1"
                     /translation="MNKYGGAIILLSFPHQLIHALAALFTDREQRRINKDAPIMIFIW
                     SYRWFDHKKNSPFLEIIKKLSKIENCQLYIPSLLQRVFGLSEFRALKQRVKYVNKIFP
                     LKDYDSFYYSHDFSADHTAQVFMQAFPNIYKVCFGDPPGFLYKNYSSVTECTEKIRSD
                     L"
     gene            complement(490..1914)
                     /locus_tag="U6309_002783"
     CDS             complement(490..1914)
                     /locus_tag="U6309_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN5506194.1"
                     /translation="MSWSIRIENLSKQYRIGSTTTTAAELINEKLRKFLLLLDFKKRN
                     RFSSDKPLPSSIAQEGRTVVDASQISPEYPNHFWALRDINLEIEAGERLGIVGQNGCG
                     KSTLLKILSRIVSPTEGLFRFRGRLISLLEIGTGFHGELTGRENIFLNGTIMGMKTHE
                     IKNRLNQIIEFSELGSMIDTPVKRYSSGMYIRLAFAVAAHLESEILIVDEVLAVGDAA
                     FQRKCTEKMLDLANQGRTLLFVSHDMETVNRICNRAIRMSHGRIVGDSKFDTSAASKS
                     SVMDITRDYLRSGAKYKSEQVWPSDKPMIFLNSLKLNRVRLIDYDNKVRSNFNLNETI
                     VVEIDFTIIKETYPFNIHLYLKDSSGRVIFVSMDNHAWQSKKVRPLGNYTERCTLYSP
                     LLNIGDYHIDIEFWPGNALENRLVVNSVVSFELKDGAVNEGVRGNWASDWPNSLIRPQ
                     LDWSISTNKKQHLVLKEHELVTEK"
     gene            complement(1911..2762)
                     /locus_tag="U6309_002784"
     CDS             complement(1911..2762)
                     /locus_tag="U6309_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN5506195.1"
                     /translation="MTSISSKTQTFKETVVYGVDKFRLLAVLRNIWDYRLLLWIFCKR
                     DLKGRYSQTILGLGWVILTPLITVGVFVIVFGIMIKVPTDGLPTVMFYLVAVIPWYSF
                     LNVLNPSIQMIEGNASLITKVYFPRALIGGAYAMGAAVDFLMAYVFLITPFAIYYGLW
                     SIKLLIIMPFLLISTLMIGLGIGLVLAPINAKYRDIKHFMPLALQLFYYSTPAIYPVS
                     AVPVWAKPWYAVNPLSLVITSYREVLMGHWPSPTIIMTLSGMAIITFLVGLVAFHHME
                     HKVVDIL"
     gene            complement(2728..>3575)
                     /locus_tag="U6309_002785"
     CDS             complement(2728..>3575)
                     /locus_tag="U6309_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506196.1"
                     /translation="FLELMGLAPEHPWLPNNSFVTKIAVESEKMFSYYQSMKFPVHQL
                     SLTGSLSDDILVEATTNGQEIKKKICQKMNLDPEKPILLCAWPTDQFGSRFVSMEFKN
                     YEQLCRAWAKCLKEIKREGKYSVIIRPHPVTNKEILGKILGQCDLKQFISYDDTIQLI
                     TCCDLFVACVSSTLRWAIAKGIPAINYDCYQYGYTDFDAKGVFNVNDFKSFSAVLEEL
                     TRDRQKYEGAKQDQQECASEWGILDGNSQKRIVNLINKLVQSESVIDIENKNYDLNIL
                     KNSDI"
BASE COUNT         1179 a          714 c          570 g         1112 t
ORIGIN      
        1 taagatctga tctaattttt tccgtacatt ctgttactga gctataattt ttataaagaa
       61 agcctggtgg atcaccaaaa caaaccttat aaatattagg gaaagcctgc atgaatactt
      121 gtgcggtatg atcggcacta aagtcatgtg aataataaaa gctatcataa tcctttaatg
      181 gaaatatttt attaacgtat tttactcgtt gctttaatgc ccgaaactca gataatccaa
      241 acactctttg aagcaaactg ggaatataaa gctggcaatt ttctattttc gataatttct
      301 taattatctc aagaaatggt gaattctttt tatgatcaaa ccaacggtat gaccagataa
      361 atatcataat tggagcatct ttattaattc tgcgctgctc tctatcagta aataatgctg
      421 caagagcatg aattaattga tggggaaaag agagtaaaat gattgcacca ccatatttgt
      481 tcatgttgat tacttttcag tgactaattc atgctctttt aaaacaagat gctgtttctt
      541 atttgtcgaa atactccaat ccaactgtgg tctaatcaat gagtttggcc aatcagaagc
      601 ccaattgcct cttaccccct catttacagc tccatctttc aactcgaagc tgactactga
      661 attgactacc aatcgatttt ccaaagcatt acctggccaa aattcaatat caatatgata
      721 atcgccaatg tttaataagg gtgaatacaa agtacatcgt tcggtataat ttcccaatgg
      781 acgtactttc ttagattgcc aggcatgatt atccattgac acaaatatca cacgcccaga
      841 gctgtcttta agataaagat gaatattaaa agggtatgtt tctttaataa ttgtaaaatc
      901 tatttcaaca acaatagttt catttaaatt aaaattagaa cgaactttat tgtcgtaatc
      961 tatcaatcta actcggttaa gcttaaggga atttaaaaat atcattggct tatcactagg
     1021 ccaaacctgt tccgatttat atttcgctcc tgagcggaga tagtcgcgag tgatatccat
     1081 gactgaactt ttgcttgctg cacttgtatc aaatttactg tcaccgacaa ttctgccatg
     1141 cgacattcgt atggctctat tacaaattcg attcacggtt tccatatcgt gactgacaaa
     1201 taataaagtt cttccctgat ttgctaaatc cagcattttt tccgtacact tgcgttgaaa
     1261 tgctgcatca cctaccgcta acacttcatc aactataagg atttcagact ccaaatgagc
     1321 agctacagca aatgccaagc gtatatacat tccggaagag tagcgcttga caggagtatc
     1381 tatcattgac cccaattcag aaaattcaat aatttgattg agtcgatttt taatttcatg
     1441 ggttttcatt cccataatag tgccatttaa aaaaatattt tcacgtccag tcagttcacc
     1501 atgaaatcct gtaccgattt ccaacaaact gattaagcgc cccctaaatc ggaataaccc
     1561 ttcggtagga gaaacaatgc gtgatagaat ttttaataat gtgctcttac cgcaaccatt
     1621 ttgtcctact ataccaagcc gctcccctgc ctcgatttca agattaatat cccgcaaagc
     1681 ccagaaatga ttgggatatt ccgggctaat ttgacttgca tcgactacag ttctaccttc
     1741 ttgtgcaatg gatgaaggca gtggcttatc ggatgaaaat cgattccttt ttttaaaatc
     1801 cagtaataaa agaaattttc tcaatttttc attaatcagc tctgctgcgg ttgtagtagt
     1861 actaccaatc cgatattgtt tggaaaggtt ttcaatacga atagaccaac tcataaaata
     1921 tcaactacct tatgctccat gtgatgaaat gcaacaagcc ccacaaggaa agttataatt
     1981 gccataccag ataatgtcat tattatagta ggtgatggcc aatgccccat taacacctct
     2041 cgataactgg tgataacaag agataatgga ttaacagcat accatggttt agcccacaca
     2101 ggcacagcag aaacagggta aatcgctgga gtggagtaat aaaataactg caaagccaga
     2161 ggcataaaat gtttaatatc cctatatttt gcattgatag gtgctaaaac caaaccaata
     2221 cctaatccaa tcattaaggt agatatcagc aggaatggca taataattaa taacttgata
     2281 gaccaaaggc cataataaat tgcaaagggg gttatcaaaa acacataagc catcagaaaa
     2341 tcaaccgcag ctcccatagc ataggcaccg cctatcaacg ctcttggaaa atagaccttt
     2401 gttattaacg aagcatttcc ctcaatcatt tgtatggaag gattgagaac atttaaaaag
     2461 gaataccaag ggataaccgc aaccaaatag aacatgacag taggcagccc atcggtaggt
     2521 acctttatca tgatgccaaa aacaatgaca aacaccccaa ctgtaatcag aggagttaaa
     2581 ataacccaac ccaatcccaa gatggtttga ctgtaacgcc ctttgagatc tcgtttacaa
     2641 aaaatccata aaagcaagcg gtaatcccat atatttctta aaacagctaa aagcctaaac
     2701 ttatcaactc catagactac tgtttcttta aatgtctgag tttttgagga tattgaggtc
     2761 ataatttttg ttttctatat caattacaga ttccgattga actaacttat taattaggtt
     2821 cactattctt ttttgggagt ttccatccag gatgccccat tcactcgcac attcttgttg
     2881 gtcctgtttg gcgccctcat atttctgcct atcacgcgtt aattcttcca aaacagcaga
     2941 aaagctttta aagtcattca cattaaaaac accttttgca tcaaagtctg tatatccata
     3001 ttgataacaa tcataattaa tggcagggat tccttttgca attgcccaac gtaaagttga
     3061 tgaaacgcaa gctacaaata aatcacaaca tgtaataagc tgtattgtat catcatagga
     3121 tataaattgt tttaaatcac actgtcctaa tattttacct aaaatttctt tattagtaac
     3181 cgggtgaggt ctaataatta cagaatattt tccttccctt ttaatttctt tcaaacattt
     3241 tgcccatgct ctacaaagct gttcgtaatt tttgaattcc atggaaacaa atcgtgaacc
     3301 aaattgatct gttggccaag cacacaataa aataggtttt tccggatcaa ggttcatttt
     3361 ttgacatatt tttttcttaa tttcctgacc gtttgttgtt gcctcaacca aaatatcatc
     3421 agataaactt cctgtcagag ataattgatg aacagggaat ttcattgatt ggtaatatga
     3481 aaacattttt tcgctttcaa cagcaatttt ggtaacaaag gaattattgg gaagccaagg
     3541 atgttctgga gctaacccca tcagttctaa aaaga
//
LOCUS       DASLOE010000232         3542 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.233, whole genome shotgun sequence.
ACCESSION   DASLOE010000232 DASLOE010000000
VERSION     DASLOE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.233"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            791..2725
                     /locus_tag="U6309_002786"
     CDS             791..2725
                     /locus_tag="U6309_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HEN5506197.1"
                     /translation="MHVLDPLTLEEIQESCDILKREKELSDSYRFAWVMTYEPPQKEV
                     LHSSDTDFDRCAFLSVFNKKTNETFEAVVNLNSKKVVEWKQIVLEVPPYGKPPILIED
                     FQKCEQIVKADPTWRAAMEKRGLDDEQIDNIQVDAFSAGYFDEEEEREQRIVKGISFY
                     RDNLKDNGYARPIEGVVAIVDLGNEKVLRVIDDGRNVPIPKEKINYDSDSYPEKRKDL
                     KPLDIIQAEGPSFKVNGWEVNWQNWSFHVGFTPREGLVLHQIKYLDEGKERPIIYRAS
                     VTDMIVPYADPSVSHYWKSAFDAGEYGLGKLANELKLGCDCLGNIYYFDVPAADDFGN
                     ARLIKNAICMHEEDAGTLWKHYETRNSTFEVRRARELVVSFFTTIGNYDYGFYWRFGQ
                     DGSLKLEIKLTGIVQTAAIFPEAQYEWGGKLTPELAAPTHQHFFNARLHMMVDGEKNS
                     FSEHEFYPIAMGGKNPYGNAFGSSQKTFEHEGEAARSACAQTQRTWKIFNPNSVNGIG
                     TAPAYKLELPETPLLLADEESYIYKRGGFASKQVWVTQYDPQEKYAAGDYPNQSSGGC
                     GLPAYIKQNRKIDNDNLVLWVTLGSTHFPRPEDFPVMPTSIISAKLQPFGFFKRNPAM
                     DLPQGKVQEKGDTCCSSLTY"
     gene            complement(2943..3131)
                     /locus_tag="U6309_002787"
     CDS             complement(2943..3131)
                     /locus_tag="U6309_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HEN5506198.1"
                     /translation="MADIINLNKKRKAKVRLEKEIKASENRIKFGRTKKEKQQEKQDN
                     ERSERHLDGHKLDKKEEK"
BASE COUNT         1100 a          641 c          752 g         1049 t
ORIGIN      
        1 gaaagtaatt ttttaactaa acttaaccac tcaaataatt agactaaata gcatttatag
       61 tatttagtaa cttatgttac acaatactta aatagtatct attttaaccc ccaatccctt
      121 gattgttttt tatgacgctt tgcaaaaaga acaagcatcc ttttaagtgt cggaatttag
      181 ggtataacca gagtgtattc acagagttat ccacagaaaa tggggataag tgattaatgc
      241 aatctaggta tcaataccta ataagtcctt gtagggcaag gggttttgta aaatgtgcag
      301 gatctcaggt ggtcagagat tgaaaaataa aaatagttat tcacaggggg gagatgcaat
      361 ttagatgtta agcgatttac atcgtaacaa aaaaaaaaat aaaaaaacag tcttgacgaa
      421 gtcgattttt ttccaaaaaa atttgggcat gtgcgattac gtataaaccg attaagccaa
      481 tgtggtaagt gtatggacaa ctatttgatt caagccgatt agaatgatat aaagtgcacc
      541 tttaatggtt gcatcgccta atgtgagcgc catgagatta aataatatac cacctttaaa
      601 aggcctgact tttaatttga caagcttatg ttgtattcac taattaaaaa ataccatacc
      661 gcacaccgcc tatccgacta ttcttagcga cataccgctt ttgtatttct atctaattac
      721 ttcaacgtgc tgtctcttta gctaacgcaa agagcaagat cggttttttt aatatgtaaa
      781 ggatgtcaat atgcatgtgt tagatccctt aactcttgaa gaaattcagg aaagttgtga
      841 tattttaaaa cgagaaaaag aactgtctga cagttatcgg tttgcctggg taatgactta
      901 cgaaccgcct caaaaagaag ttttgcattc aagtgatact gatttcgacc gttgtgcttt
      961 tctctctgtt tttaataaaa aaacgaatga aacctttgaa gcagtcgtaa acctcaacag
     1021 taaaaaagtt gtggaatgga aacagattgt ccttgaagtg ccaccctatg gtaagccgcc
     1081 tattttaatt gaggattttc aaaaatgtga gcaaatcgtt aaagcagatc cgacctggag
     1141 agccgcgatg gaaaagcgcg ggcttgatga tgaacaaatt gacaacattc aagtagacgc
     1201 attttctgcc ggctattttg atgaagagga ggagagagaa caacggattg tcaaagggat
     1261 aagtttttac cgtgataatt taaaggataa tggttatgcc cgtcccattg aaggggttgt
     1321 cgcgatagta gaccttggca atgagaaggt attgcgtgtc attgatgacg gccgcaacgt
     1381 cccaattcct aaagaaaaaa tcaattacga ttcggattct tatcctgaaa aacgcaaaga
     1441 cttaaaaccg ctggatatta ttcaagcaga aggaccaagt tttaaagtaa atggctggga
     1501 agtcaattgg caaaattggt cattccatgt tgggtttacg ccgcgtgagg gattggtttt
     1561 gcaccagatc aaatacctgg atgaagggaa agaaaggcca attatttacc gggctagtgt
     1621 gacggatatg atagtcccct atgcggaccc ttccgtgagt cattactgga aaagcgcttt
     1681 tgatgcgggt gaatatggtc ttggcaaatt ggcaaatgaa ctaaaactcg gatgtgattg
     1741 tttaggcaat atttattatt ttgacgttcc tgcggcggat gacttcggca atgccagatt
     1801 gattaaaaat gcgatttgca tgcacgagga agacgcgggc accctgtgga agcattatga
     1861 aacacgcaac agcaccttcg aggttcgccg tgctcgagag ttggtcgtca gcttttttac
     1921 tacgataggt aactacgact atggctttta ctggcgattt ggacaggatg gcagcctcaa
     1981 actggaaata aaactgactg gtattgtaca aacagcggca atatttcctg aggctcagta
     2041 tgaatgggga gggaaattga caccagagct ggcagcacct acgcatcagc attttttcaa
     2101 tgcccgcctt catatgatgg ttgatggtga aaagaactca ttcagtgaac acgagtttta
     2161 tcctatcgcc atgggtggaa aaaatcccta tggaaatgcg tttggcagca gccagaaaac
     2221 atttgaacat gaaggtgagg cagcccgctc ggcttgtgcg caaacccaga gaacctggaa
     2281 gatattcaat cccaatagcg tcaatggcat tggcacagca ccagcttata aactggaatt
     2341 gccagaaacc cccttactgc tggcagatga agagagttat atttataaac gcgggggatt
     2401 tgccagcaag caggtgtggg taacacaata tgacccacag gaaaaatacg cagcaggaga
     2461 ttatcccaat cagagttctg gtggctgtgg tttacctgct tacatcaaac agaaccgtaa
     2521 aatagacaat gacaatttgg tcttgtgggt aacgctgggg tctacgcatt ttccaagacc
     2581 tgaagacttt ccggtcatgc ctacttcgat catttctgcc aagcttcagc cttttggttt
     2641 ttttaagagg aatccggcta tggatcttcc tcaaggcaaa gtacaggaaa aaggggacac
     2701 ctgctgttct tcattaactt attaatggat tggaactaag caccttcttg aggagagggt
     2761 gctttcctgt tggagcttaa aaatcaaaat ctctcaggac ttacgaaaaa ccccaatctc
     2821 tatgttaaaa aaagttttta attcggtcat ttagtttatc taaactccct cattaaaaac
     2881 attttttgcc ttgaccttgg agtttttcgt aagtcctgtc gctattaaaa gagttcctgt
     2941 ctttattttt cctctttttt atccagcttg tgaccatcca gatgacgttc actgcgttca
     3001 ttgtcttgtt tttcttgttg cttttctttt tttgtccttc caaattttat gcgattttca
     3061 gatgctttta tttctttttc caagcggact ttagcctttc tttttttatt aagattaatg
     3121 atatctgcca tggcactctc tgttgtaaga ccttgtttat actatagcgc ataaaaccag
     3181 ggtaaatcat ctcaatacac aaatcgatcg aaatggaatg atctgaaagt atacactttg
     3241 agaatgggaa ggactgtcgc aagatagtat tttcagcatt gttttttatg gcactttgca
     3301 aaaagaacaa gcatcctttt aagtgcagga ttttagtgta taaccagagt gtattcacag
     3361 agttatccac agaaaatggg gataagtaat caatacaatt cacgtattaa tatttaataa
     3421 atctttacag ggcaaggggt tttataaaaa gagcatggtg tcaaatgcta ataagattaa
     3481 aaataaagac aagttatcca cagaaagcga gatgctagtg aaggtatggg acgctcatta
     3541 tg
//
LOCUS       DASLOE010000233         3492 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.234, whole genome shotgun sequence.
ACCESSION   DASLOE010000233 DASLOE010000000
VERSION     DASLOE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.234"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            158..3424
                     /locus_tag="U6309_002788"
     CDS             158..3424
                     /locus_tag="U6309_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN5506199.1"
                     /translation="MLNEAISKMPEAFEAKILLRGEEYFQKGQVLNIRLSDGLLKGRV
                     KGSASQIYDIHMDLKSWPAKPSRCSCAYQYNCKHAAACLFALRDREKINLQSLPSNKL
                     DRKLDVWLKNLRAQEASEIKREEATHHLIYLIDLKLRGYEHKVSIKLALAKLLKRGGY
                     GKKIIFNTLANSKKQHFMGDDNDIVAQLLFKCNVSGWFDNLLIRNTELLDKIIATGRA
                     FFIENEDIPIKKGETLTGECQWILSWNGNQILKLRHDNQNIEPLLLDESWYFDIKNAV
                     IGHLNSPYPIKQLRHLLEAPPIPLDQAELLAKKMSKNCPEFPMPNVFNQKETKQLKPI
                     PVLILDAVTEQEEHRWIDDGEENNSLYIAKIFFDYEGLLVPFAEECEKVVSLKGDVLV
                     EYQRDQEFEDLKIQELRNSIDLRPFHQWEKYHWNLPDHSFFILKDVQIVSDLESLYQQ
                     SLPYLKDHGWRIEFASPFYQEIVSADEVEWYSDIQESTTDFFSYQLGILVEGKPVSIV
                     PLVADLIQRYKGNDLDNLPDEQLVKLPLLDGRLLQLPMGRIKPLVRLLLQFGLRQIDQ
                     NQQLQINKYQLVLMQEVELAIAATKSRWQGAESLREELRKLVRLNDLPEIKVPSGLQA
                     HLRDYQHYGLNWLQFLRVSRFNGVLADDMGLGKTVQTLAHLQYEKEQSRLHKASLIIA
                     PTSLVGNWFAEAKRFTPKIKALIYHGSDRHQDNFDDYDLIISTYGLIHRDKEKFVGYP
                     FYYLILDEAQFIKNARTKTTQIIQQLKASHRLCLTGTPLENHLGELWSLFHFLMPGLL
                     GDAKQFRLWFRTPIEKYADMGRRDLLAKRVQPFLLRRTKNQVANELPPKTEITRTIEL
                     VGAQRDLYEAIRMSMEKKVRDAIARQGLGKSHILLLDALLKLRQVCCDPRLLSMSEAE
                     IAHGTSCKLDALMELLDNLVEEGRRVLVFSQFTSMLKLIEELLIARQYDYLKLTGQTQ
                     NRQALVEQFQQGDTPIFLISLKAGGTGLNLTRADTVIHYDPWWNPSVEDQATDRTHRI
                     GQESPVFVYKLITSGTVEEAILGMQEKKRQLVEGILSTDASRSMVLSEEDLNQFFMPL
                     "
BASE COUNT         1118 a          570 c          766 g         1038 t
ORIGIN      
        1 atggtcgcgg ctcggattat ggttcactct gagtcagata ggatcatggt taaaaccgag
       61 ccgcaaccgt aagggagcgg atagaacaac ccattctatt gtcaaaaagc gtaaaaaatg
      121 actacaatat acgatttttc ctggacaaat cctaggtatg ttaaacgaag caatatcaaa
      181 aatgccagag gcttttgaag ctaaaatatt gcttcggggt gaagagtact ttcaaaaagg
      241 ccaggtattg aatattcgct taagtgacgg cttgctcaaa gggagagtga agggtagtgc
      301 aagtcaaatt tatgatatcc atatggattt aaaatcatgg ccagcaaaac cttctcgatg
      361 ttcatgtgct tatcaatata attgcaaaca cgccgcagct tgtctttttg cattaagaga
      421 cagagaaaaa ataaacctgc agtcactgcc ttctaacaaa ctagatcgaa aattagatgt
      481 ttggttaaaa aatttacgag cacaagaagc atcagaaata aaaagggagg aggcgacaca
      541 ccatctgatt tatttgattg atttaaagct tcggggttat gagcacaaag taagcattaa
      601 attagctttg gccaaactat taaaacgcgg cggttacgga aaaaaaataa tctttaatac
      661 tctggctaat tcaaaaaaac aacattttat gggtgatgat aatgacatcg tggctcaatt
      721 gttatttaaa tgtaatgtat ccggttggtt tgataatctt ttgattcgga atacagagct
      781 tcttgataag atcattgcaa ctggaagagc tttttttatt gaaaatgagg atatccctat
      841 aaaaaaggga gagactttga ctggtgaatg ccagtggatt ttatcctgga atggcaatca
      901 gattttaaaa ttaaggcatg ataatcagaa tattgagcca ttattattgg atgaaagttg
      961 gtattttgat attaaaaatg cggtaatagg tcacttaaac tctccttatc ccatcaagca
     1021 gcttcgtcat ttgttggaag caccacctat ccctcttgat caagcagagt tattggccaa
     1081 aaaaatgtct aaaaattgtc ctgaatttcc catgcccaat gtgtttaatc aaaaggaaac
     1141 gaaacaatta aaaccaattc ctgtgttaat tctcgatgcg gttaccgaac aggaagaaca
     1201 caggtggatt gatgacggag aggaaaataa ttctctttat atagcaaaaa tcttttttga
     1261 ttatgaagga ttgcttgttc cttttgcgga agagtgtgaa aaagtggtgt ctttaaaagg
     1321 agatgtatta gttgaatacc aacgtgatca ggaatttgaa gatttaaaaa ttcaagagtt
     1381 gagaaattca attgatttga ggccatttca tcaatgggaa aaataccatt ggaatttacc
     1441 cgatcactcc ttttttattt taaaagatgt gcaaattgtt tcagacttgg aaagtcttta
     1501 tcagcaatcg ttaccttatt taaaagacca tggatggcgt atagagtttg caagcccgtt
     1561 ttatcaagag attgtttctg ctgatgaagt ggaatggtat tccgatattc aggaaagtac
     1621 gaccgatttt ttttcctatc aattgggtat tttggttgaa ggcaagccag tgagtattgt
     1681 gccgctggtt gcggatttga ttcaacgtta caaagggaat gatttagata atttacctga
     1741 tgagcagctg gttaaactcc cattgttgga tggtcgttta ttgcaattgc ccatggggcg
     1801 aattaagcca ttggtccgtt tgttattaca gtttggcttg cgccaaattg atcaaaatca
     1861 gcaattacag attaataaat atcaactggt attaatgcag gaagttgaat tggctattgc
     1921 tgcgaccaaa tcacgttggc aaggtgctga atcattaaga gaagaattaa ggaagctggt
     1981 cagattaaat gatctgccag aaataaaggt tccatctggt cttcaagctc atttgaggga
     2041 ttatcaacac tatgggttaa attggttaca atttttgcgg gtcagtcgtt ttaatggcgt
     2101 attagcggat gatatgggac ttggcaaaac agtgcagact ctcgcccatt tacaatatga
     2161 gaaagaacaa tcaagattac ataaagccag tttaatcata gctcccacca gtcttgttgg
     2221 taattggttt gccgaagcca agcgatttac gccaaagatc aaagcattga tttaccatgg
     2281 aagtgataga catcaggaca attttgatga ttatgattta attatttcta cctacggatt
     2341 aattcacagg gacaaagaaa aatttgttgg ataccccttt tattatctca ttttggacga
     2401 agcgcaattc attaagaatg cacggacaaa aacaacacaa atcattcaac aattaaaggc
     2461 ttcacaccgt ttatgtctta ctggaactcc actagagaat catttaggcg aactctggtc
     2521 tttatttcat tttctaatgc ccgggctatt gggtgatgca aagcaattca ggctttggtt
     2581 cagaacgcct atagaaaaat atgccgatat gggaagaagg gatttgttag caaaaagagt
     2641 gcaacctttt ttactgcgcc gaaccaaaaa tcaggtagcc aatgaactcc cgcctaaaac
     2701 ggaaataact cgtactatag agttggttgg tgctcagcgg gatctttatg aggctatccg
     2761 catgagcatg gaaaaaaagg tgagagatgc catagcaagg caaggattgg ggaaaagtca
     2821 tatattatta cttgatgcct tgttgaaatt gcggcaggtt tgttgtgatc caagattgtt
     2881 gtctatgtct gaagccgaga tcgctcacgg tacttcatgc aagctcgatg cactgatgga
     2941 gttgcttgat aatttggttg aagagggaag gagagtattg gtattttcac aatttacatc
     3001 gatgctaaaa ctgattgagg aattattaat agccaggcaa tacgattatt tgaaattgac
     3061 gggacagacc caaaacagac aagccttagt cgagcaattt caacaaggtg atacgccaat
     3121 atttttaatt agtctgaaag caggaggcac aggacttaat ctgactcgtg cagatactgt
     3181 gattcattat gatccctggt ggaatccttc tgttgaggac caggctactg acagaacaca
     3241 tcgaataggc caggaaagcc ctgtttttgt ttacaagttg attacatcag gtactgtaga
     3301 agaggcaatt cttggtatgc aagagaagaa aaggcaatta gtggaaggga ttttgtctac
     3361 agacgcttcc aggtcaatgg ttttaagtga agaagatttg aatcaatttt ttatgccttt
     3421 atgaataaaa gtaacggttt attatccgct ccctgacggt cgtggctcgg tttgatggtt
     3481 gaggattggt tt
//
LOCUS       DASLOE010000234         3459 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.235, whole genome shotgun sequence.
ACCESSION   DASLOE010000234 DASLOE010000000
VERSION     DASLOE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.235"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            50..850
                     /locus_tag="U6309_002789"
     CDS             50..850
                     /locus_tag="U6309_002789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506200.1"
                     /translation="MAHYLRDQQITNVSIGESEIEQITTLFIRQVNTLNGLIPKGDDG
                     AKTPIFYFTIRFDGKGYRVFSQEELLAHFKQAKEVERIIFTLETGESIRTVRNIGVVI
                     ELKLDQNNPGNCSLVVTSDDQEMVNTVFFSFQEVLMNCKNKYGFVRTAWMELFVQIVG
                     IAFGFIFSLWAAVKIAPRISIENSFVICFFFIFLLFSNTWSYLSKLIHGFLNYIFPNV
                     YFYRPDKHRVTWLMQIIVTAIIGAISLWILGALSSYFIGIISGFVVKN"
     gene            1072..1326
                     /locus_tag="U6309_002790"
     CDS             1072..1326
                     /locus_tag="U6309_002790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506201.1"
                     /translation="MYIIYFDEVKNRPHKQRYYWLGALAIPHDLALDLETKVNDISNE
                     VFGSPLLSKDTEFHGNEIICGTRVRSCLLPLKNKKAKSKI"
     gene            complement(1522..1875)
                     /locus_tag="U6309_002791"
     CDS             complement(1522..1875)
                     /locus_tag="U6309_002791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506202.1"
                     /translation="MRSAKILVTGNNEIDFRNATSRAYYSALHKADIVAAKSNGISKK
                     NSGLHAQLIQKLKSHTGSTIIDDNIRQIGVLLGMCKKYRVNADYKINHTIDRSFAQQT
                     ISLVEQLLILSNQIP"
     gene            complement(1851..2201)
                     /locus_tag="U6309_002792"
     CDS             complement(1851..2201)
                     /locus_tag="U6309_002792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506203.1"
                     /translation="MNRFNQLNTNEPELNNKLVNALKAIGVGICKKYLVDLSDINYEK
                     VVFDNDTWININFVVDCIDQNQLSKLNIELSKELAKYTEFLNSDIEYIPSFTQGEIKC
                     QSHTVNSCAPQKYL"
     gene            2367..2567
                     /locus_tag="U6309_002793"
                     /pseudo
     CDS             2367..2567
                     /locus_tag="U6309_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1130 a          541 c          656 g         1132 t
ORIGIN      
        1 taatggatag taaaatcgaa aatacatatt tacaagggat aaattaaaga tggcgcatta
       61 tctaagagac cagcaaataa ctaacgtcag cattggcgaa agtgaaatag agcaaataac
      121 tacattattt ataagacaag ttaatacact gaatggattg attccaaagg gtgatgatgg
      181 agccaaaaca ccaatttttt attttactat ccgatttgat ggaaaagggt atcgtgtctt
      241 tagccaagaa gaattattag ctcattttaa acaggcaaaa gaggtagagc gtattatatt
      301 cacgcttgaa acaggggaga gcataagaac agttcgcaat attggtgtgg tcatagaatt
      361 aaagcttgat caaaataatc caggaaactg ttcgttagta gtcacatctg atgatcaaga
      421 gatggtgaat actgtttttt tctctttcca agaggtttta atgaattgta aaaataagta
      481 tggatttgta cgtactgcct ggatggagtt attcgtgcaa atagttggga tagcatttgg
      541 gtttatattc agtttatggg cagcagtaaa aattgctccc agaatttcta ttgagaattc
      601 ttttgttatt tgtttctttt ttattttcct tcttttttca aatacatggt catatttaag
      661 caaactaatt catgggtttt taaactatat ttttcctaat gtttattttt atcgccctga
      721 taagcatcgg gtgacttggt taatgcaaat aatagttacc gcaataattg gggcaatttc
      781 gctctggata ttgggggcgt tgtcatctta ctttattggt attatttctg gctttgttgt
      841 aaaaaattag tagagtttat tggaataggg cagagatgta atttatctta gcgatatttt
      901 gtggccataa aagggagtca actaaaatag cgcatgttgg gttgtttctc tattccatta
      961 tgaaagtgtg tataacagtt ttgattttag tgtgcaagaa gataaaggaa atatcgggcg
     1021 atgcgctcaa cctacggttg ctataatact ctgtattaca gggatagtat tatgtatatc
     1081 atttatttcg atgaagttaa gaacaggcct cataagcaaa ggtattattg gttaggtgcc
     1141 ttggctatac ctcatgactt agcactagat ttagaaacaa aagttaacga tatttcaaat
     1201 gaagtatttg ggagcccatt actatcaaag gatactgaat ttcatggtaa tgaaattatt
     1261 tgtgggacta gggtcagatc ttgccttttg cctcttaaaa ataaaaaagc aaaaagcaag
     1321 atctgaccct gaacgctgct tcgatatcca atttaaagga actattataa tgggatggct
     1381 aaagcctaat agtgtcgtgc ttgtcccaaa tgtctctttt gtccctgagg aattggatag
     1441 catgtttctg cgaatgtatt tcataaaaat aaaatgctaa tcaatcgagt atgacctcat
     1501 tgatttttca atcaagactg gctaaggaat ttgattggat aaaattagaa gctgttcaac
     1561 taaagaaatt gtttgctgtg caaaactcct atcaattgta tgattgattt tataatcagc
     1621 attcactcta tactttttgc acatccctag tagaacgccg atttgcctga tattatcatc
     1681 aattatggtt gagcctgtat gtgattttag cttttgtatt aattgtgcgt gtaaacctga
     1741 attcttctta gatattccat ttgatttagc agctactata tccgctttat gcagtgctga
     1801 ataataggct ctacttgtag catttctaaa gtcaatttca ttatttccag ttacaagtat
     1861 ttttgcggag cgcatgaatt cactgtatgt gactggcact ttatttctcc ctgagtaaat
     1921 gaagggatgt actctatgtc tgaattaaga aactcagtat attttgctaa ttctttgcta
     1981 agttcaatat taagtttaga tagctggttt tgatcaatgc agtcaactac aaaattgata
     2041 ttaatccatg tatcattatc aaaaactact ttttcataat taatatcaga tagatcaacc
     2101 agatactttt tacaaatacc aacaccaata gctttaagag cattgacaag cttgttgttt
     2161 agttcaggtt cgtttgtatt taattgatta aacctattca tactaattcc tacagtagta
     2221 tatgcataaa ttatagacta tattgttgta agtatgtaag caaaaacata ggtcgaattg
     2281 cctcataaaa aatgttaccc aaaataggaa attttagatt cgttgcctca taaataatgt
     2341 taccgaagaa aaaaagggac atagaaatgg ggaagctttc aatggatctt tatttggaca
     2401 atatcattag gcggtatcat gctgctaccc ggaaagaaaa gagcatgata ttaaatgagc
     2461 tctgtactgc aagcggttac cacaaaaaaa acgcaatacg attgttaaat caaaagaaac
     2521 agcccaataa agctgataaa aaaagacggg gacgcccacc ccagtataaa tgtccagttt
     2581 atttagagcc attaaaaaag atatggctat gcacggaaca gttatgtggg aagcggttaa
     2641 aaatggccct accattatgg ttgccgcatt atggagctat ttatggcgaa ttagaccctg
     2701 taatttatga gggcttatta aagatgagtg cctctacaat agatcgtatg ttatccagtt
     2761 cacgtgttaa gggtaaacga gggttgagcg gaacaaaacc gggtaccatt ttaaaaaaac
     2821 atattccgat aaaaacagat caatggaatg aagaaaagcc tggcttttta gaagcagata
     2881 ctgttgccca ctgcggttca tctttagcag gggatttcgc atggagttta acaatgaccg
     2941 atatgtgttc gacatggact gaaaatagag cgatatggaa taaaggagcg ataggtgtct
     3001 tagctcaaat agaggatatt gaaaagaacc ttccctttga tattttagga ttcgattcag
     3061 acaacgtact aaatctggag tcaatgattt ttttgaaaaa aagcaatccc attcgctaaa
     3121 aagcgatttt tttaattttt agtttgcaaa aacttataca agttcctcaa taaatcccaa
     3181 tggtttccat tttttatagg agttttttta accatagtga cacaaggtcc atagcaaatt
     3241 gccaagcctt tactgtactt aaaacaaaat aaagtcccaa tggaagatcc aaacattatc
     3301 tacgaggtct ataagcctct acggccctat tgaagggatt ctcccactgg gtattaccga
     3361 ttcatggtaa catcgataat tcgtttcatt tttaatgata ccataagcca cacgtgctat
     3421 tttggcagca actgcagtat atccttttcg ttttaaatc
//
LOCUS       DASLOE010000235         3449 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.236, whole genome shotgun sequence.
ACCESSION   DASLOE010000235 DASLOE010000000
VERSION     DASLOE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.236"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(172..1965)
                     /locus_tag="U6309_002794"
     CDS             complement(172..1965)
                     /locus_tag="U6309_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5506204.1"
                     /translation="MALSEIIINDVVQYQTNAVISESNYHILRQYLLERKPLSRVLNP
                     INSLLSEHCDKDKQAIINHLTQLACESQRNYDIQEAMSDEQERVNENSLMINYRSELL
                     TLENTLKQLDTKCFQQQRQYNQVSRQLNELKVNLTHINQSIDRIRYERQLLDQQLNHP
                     YSDGNVYTNTPTTTRIYPDLSLPQAPLARDRLLQEENRLIEERQRLMHLVNSKEAEKD
                     KEEQNLNRLHKEKKETEGRYSEVKHQIDIALPNSEQQRQIRNQERLAREQARASYDPH
                     LLQLSHKNLEALKHEIEIQTRELDERRNQLMSEAKEMSYKIYLTQLEQVLQESENGPQ
                     ITFNENNALKMILAMMKNMEEMAEKEKDITSSIEKERHNLRSLQKSLVAYTRQLEQYL
                     TADPHLVKQNKALTEENAQLQQYSESAASWRTKAFYASLFSIGGSLISTGILNTFIIS
                     PVFFAIPGALATLGVVSIVIVVVSHCQKYFSDVQMEINDQKIEKNELVLMKQWKKANE
                     LSLSTIPGLNAKIEKSEKELYALEQKLQDQQHAMDLLLNKAQNITSNYYRSSNFFGNT
                     TGNVLYLPSAPLHEDSPLYPEIEGERVNYGY"
     gene            complement(2408..3031)
                     /locus_tag="U6309_002795"
     CDS             complement(2408..3031)
                     /locus_tag="U6309_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEN5506205.1"
                     /translation="MYTLYSFGTPNGFKPTIMLEELAQPYTIKLIDITQGEQFNPDFL
                     KISPNNKIPVLHDQTNDFYLMESVAILQYLAEKHRQFLPETTKEKYAIIQWCYFQAAH
                     IGPMLGQFGHFHRYAQEQVPYAMKRYADESMRLLGVMEKQLAKTSFIGGSNYTIADMA
                     IWPWIWCFQFIYEQTIDTTLFPSLMSWYQRVSERPAVRATLAAYGRL"
     gene            complement(3066..>3449)
                     /locus_tag="U6309_002796"
     CDS             complement(3066..>3449)
                     /locus_tag="U6309_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN5506206.1"
                     /translation="GGRNGHVETIDGLLKLDLAMPRELGGEGRATNPEQLFAAGYAAC
                     FESAIRHVANVQKISLEDVSMTSEVSLYATPEKGFKLGVALHAYITGLNQNEAEALVA
                     KAHEVCPYSNAIRGNVEVKLSVSVK"
BASE COUNT          985 a          686 c          626 g         1152 t
ORIGIN      
        1 cctgaaatca gctccagccc tcataaacca cccagcctta acttcatttt aaatccagta
       61 tgatcaaaat atttttacaa agcagattgt aaagagcaat tcagaatttg aataatcaga
      121 gatactggtc ttcaaattga aaccaaatta aagaccagtc attttagctt attagtagcc
      181 gtaatttact ctttcacctt caatctcagg ataaagtgga gagtcctcat gtagaggggc
      241 agaaggtaaa tagagcacat taccagtagt gttaccaaag aaattgctgc ttctgtaata
      301 attgctggta atattttgcg ctttattcaa cagcagatcc attgcgtgct gctgatcttg
      361 taatttttgt tcaagcgcat acagctcttt ctctgatttt tctattttag cgttcaggcc
      421 tggaatagta gataaactta attcattagc ctttttccat tgcttcatta gtactaattc
      481 atttttctca atcttttggt catttatttc catttgcaca tcactaaagt acttctggca
      541 atgagagact acaacgataa caatggaaac tacaccaagc gtagcaagag cgccgggaat
      601 cgcaaaaaat acaggactaa tgatgaatgt attcaatatt ccggtactta ttaagctacc
      661 cccaatactg aaaagagacg catagaatgc tttcgttctc cagctggcag ctgattcact
      721 atactgctgt aactgcgcat tttcttcagt aagagccttg ttttgtttca ccaaatgggg
      781 atctgccgtc agatactgct ctagttgcct agtgtaggct acaaggcttt tttgcaatga
      841 acgaagatta tgtcgctctt tttcaattga gcttgttata tctttctctt tttccgccat
      901 ttcctccata tttttcatca tggctaaaat catctttaag gcattatttt cattgaacgt
      961 gatttgaggc ccattttctg attcctgaag aacctgttcc aattgagtta gataaatttt
     1021 ataactcatt tctttcgctt cgctcattaa ctgatttcgc cgctcatcca gttcccgagt
     1081 ctgtatttca atctcatgct tcaatgcttc aagattttta tgtgatagtt gcaataggtg
     1141 cggatcataa cttgctctgg cctgttctcg agcaagacgc tcttgatttc tgatttggcg
     1201 ttgttgttca ctatttggca aagcaatatc gatttgatgt tttacttcac tataacgccc
     1261 ttcagtctct tttttctcct tatgaagtct atttaaattt tgttcttctt tgtctttttc
     1321 agcctctttt gaattgacaa gatgcatcag ccgctggcgc tcctcaatca atctattttc
     1381 ttcttgcagc agtcggtctc gcgcaagcgg agcctggggt agtgaaagat cgggatagat
     1441 acgtgtggta gtaggagtat tagtatacac attaccatcc gagtaaggat gatttagctg
     1501 ttggtcaagc aattgacgtt catatcggat tctatcaata ctttgattaa tgtgggtcag
     1561 attgacttta agttcattaa gttgcctgct cacttgattg tattggcgtt gttgttgaaa
     1621 gcattttgta tccagttgct tcaatgtatt ttccagggta agcagctcgc tcctgtaatt
     1681 aatcattaaa gagttttcat tgactctctc ttgttcatca ctcattgcct cttgaatatc
     1741 ataatttctt tgagattcac aggccagttg agttaagtgg tttattattg cttgtttatc
     1801 cttatcacaa tgctcagaaa gcagagaatt gattggattg agtactcttg ataaaggctt
     1861 tctctccagg agatattggc gaagaatatg gtaatttgat tcgctaatga ctgcgttggt
     1921 ttgatattga actacatcat tgataatgat ttctgataac gccatgactc gtgactcctc
     1981 agtaattata ttattaacga actcgtttat tgaaactatt aatgtgaatc gctgcatgat
     2041 gcagaagaaa tttgctcccc ctgaaaatac agactacatt tcctagttct attcaaagca
     2101 gtgtgataaa tcctcctgta ttttattctt ttgtttaaac tgagggcatt atggcagaca
     2161 aatgttaagg aattgttaag agattgttaa ggaattgtta aaaattatca gaattagaga
     2221 ataaacttag ttttaaatag atatgaaaga caatagagat tatttgattg acagctgcat
     2281 aatctcaatt tttctaatgt cacaatttat tgaggcaata accaggtaaa acatcccaat
     2341 tgacagcatt atggcaattg ggagcaatta attatcgata ggttccacta atattgccta
     2401 tcatctatca taatcgacca taggcagcta gagtcgcgcg aacagcaggc ctttcactaa
     2461 ctcgttgata ccaggacatt aaactgggaa acagcgtcgt atcaatagtc tgttcatata
     2521 tgaattgaaa acaccaaatc cagggccaga tagccatatc ggcaatagta taatttgatc
     2581 cgccaatgaa tgacgttttg gccagttgct tttccatgac ccctagaagt cgcatggatt
     2641 catcagcata tcgcttcatg gcatagggta cttgctcttg agcataacga tgaaaatgac
     2701 caaattgccc taacataggg cctatgtgtg ctgcctggaa ataacaccat tggataatgg
     2761 catatttctc ctttgtcgtt tcaggcaaaa actgtctatg tttttcagcc aaatattgca
     2821 aaatagcaac actttccatt aggtaaaaat cattggtttg atcatgcaaa accggtatct
     2881 tattgtttgg tgaaattttt aaaaaatcag gattaaattg ttcaccctga gtaatatcaa
     2941 tcagttttat ggtgtaaggc tgtgccaact cttccaacat aatagtgggt ttaaatccgt
     3001 tgggcgtccc gaatgaataa agtgtataca ttaagatatc cttattaatt gatggaagtt
     3061 atctatcact tgacactaac actcaatttc acttcaacat taccacgtat tgcattggaa
     3121 taaggacaaa cctcatgcgc cttggcaact aatgcttcgg cctcattctg atttaggcct
     3181 gttatatagg cgtgtaatgc aactccaagt ttaaaaccct tctctggagt agcatataaa
     3241 gagacctcgg aagtcatgga aacatcctct aaagaaattt tttgcacatt ggcaacatga
     3301 cgaatggcac tctcaaaaca agcagcatat cccgcggcaa ataattgctc tggattagtg
     3361 gcccgaccct ctccccctag ctctctcggc attgccaaat caagttttaa tagcccatct
     3421 atagtttcta catggccgtt tcgcccacc
//
LOCUS       DASLOE010000236         3380 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.237, whole genome shotgun sequence.
ACCESSION   DASLOE010000236 DASLOE010000000
VERSION     DASLOE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.237"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1265
                     /locus_tag="U6309_002797"
     CDS             <1..1265
                     /locus_tag="U6309_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEN5506207.1"
                     /translation="INQSDSVERNPLASQYINSIGKKLAHYGRIKTPYFFIVKSKEIN
                     AFAGPGGYIGINTQLILTSSNESELAAVMAHEIAHVRLHHLYSIIEHQKQMRVPLLAS
                     ILASAALGVLNPTVGMGAMMASVSGFSQDNINFTRSKEKEADRIGIDMLIKAGFNPQG
                     MAAFFNKMQENSRYYYTANIPAILRTHPLDADRIAEAENRTSRLPKKHYTDSLDYALF
                     KELIRVSVASNNKQILDYYEHHCHKKNPATACQYGYVLALLNNNNFQKAAEKLNPLLL
                     QDNSNLNFQIAMAQAELGLSQYQSALSRLAELQNNFPDNYAALMAYAQGLLTANQPQK
                     ATAVLLKGSRQYKEDLPLCRELARAQAESHQKSYAYFTQAQCLLLEGQKRGAIAQLKV
                     AQKLAKKDAYLSARISAKIDEIKFMVEN"
     gene            1544..2479
                     /locus_tag="U6309_002798"
     CDS             1544..2479
                     /locus_tag="U6309_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN5506208.1"
                     /translation="MNEIQEFLRQKLNSPLPISRPQLAKLPMGTMEFVTYGNGKPVLI
                     IHGGGGGYDQAVLLFKRYIPDGYQMICPSRPGYLGTPIDTGQTAEEQADALAGLLDYL
                     NITSAVIVAISAGGLSLYPFAIRHPKKTKAIIAIDAIAGEYLMPEQSNKLAQALFMTN
                     FGLWVAKKSMIYFPKSFIKNLIKLEGYLDPVRVDDRVKEILNCRERLEIIAELMNSMA
                     DYKARKTGTVNDMAEGVKTSWYDFAKINCPALIIHGTHDADVKFYNGVFAYERLASKE
                     KERFWIEYGTHFGFFFARQAREVQQKFQGFIIKHG"
     gene            complement(2917..3162)
                     /locus_tag="U6309_002799"
     CDS             complement(2917..3162)
                     /locus_tag="U6309_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506209.1"
                     /translation="MNSWNSVVFLKTKNAWSDSAAIAKMNGVQSLWSTSGDWDWCIKL
                     DSKHSSPEETALFVKNLRNADWVAETKTSWWKEVAVK"
BASE COUNT         1106 a          638 c          630 g         1006 t
ORIGIN      
        1 ttattaatca atcggacagc gtagagcgca atcctttggc cagtcaatac atcaactcca
       61 taggaaaaaa actggcacat tatggccgta tcaaaacacc ttattttttt attgttaaat
      121 ccaaagaaat caatgcattt gcaggtccag gagggtacat aggcattaat acccaactca
      181 ttttaacgag cagcaatgaa agtgaactgg cagctgtaat ggctcatgaa attgcgcatg
      241 ttcgcttaca tcatctttac agcattatag aacaccaaaa acagatgcgt gttccattat
      301 tagcctctat tctggcgtct gcagccttgg gcgtcttgaa tccaactgtc gggatgggcg
      361 ctatgatggc atccgtttcc ggattttccc aggacaacat caattttaca cgctcaaaag
      421 aaaaagaagc cgacagaatt ggtatagaca tgcttattaa ggcaggcttc aatcctcaag
      481 gtatggccgc attttttaat aaaatgcaag aaaattcccg ttattattac actgccaata
      541 ttccagcaat actgcggact caccccctgg atgctgacag gatagctgag gctgaaaaca
      601 gaaccagccg tttgcctaaa aaacattata ctgattcttt ggactatgcc ttatttaaag
      661 agttaatcag agtttctgtc gcctctaata ataaacaaat tcttgattat tatgagcatc
      721 attgccataa aaaaaatccc gccactgctt gccagtatgg atacgtactt gccttgttaa
      781 ataataacaa ttttcaaaaa gcagctgaaa aattaaaccc tttgctgtta caggataata
      841 gtaatctcaa ttttcaaatt gctatggctc aagctgaatt aggtttatca caatatcaat
      901 cagcactctc ccgtcttgct gaattacaaa ataattttcc tgacaattat gctgctctaa
      961 tggcatacgc ccaaggattg ttaaccgcaa accaacccca aaaagctaca gccgtacttc
     1021 ttaagggtag tagacaatac aaagaggact taccactttg ccgagaattg gcaagagctc
     1081 aagctgaatc ccatcagaaa agttatgctt attttactca agcgcaatgc ttactacttg
     1141 aggggcaaaa aagaggtgct attgctcaat taaaagtggc gcagaaactg gccaaaaagg
     1201 atgcttattt atccgcaaga atttcagcaa aaattgatga aataaaattt atggttgaaa
     1261 actaatctaa atatagatta attaaaataa aatgatctca tttatcattc gctaagagtg
     1321 taagggttta attttgtttt gttcaggccc aatataatta attaatgctt gtgagcatct
     1381 catttctgat aggtcttaaa tggaaataaa atagaacata tttatcaaaa agaaattaga
     1441 tggcaaaaag gtctcttcat aaataatttt cgatattttc agtagacaag atcatctaaa
     1501 aagccaagta ttaatttact ttcactttgg atagggagtt gccatgaatg aaattcaaga
     1561 attcttgcgt caaaaactga attcgccgtt acctatttca cgacctcaat tggcaaagct
     1621 accaatgggt actatggagt ttgttactta tggtaatgga aaacccgtac tgattattca
     1681 tggtggaggg ggtggttatg atcaggcagt gcttttattc aaacgctata tccccgatgg
     1741 ttaccaaatg atttgtcctt ctcgtcctgg ttatttggga acacccattg atactggaca
     1801 aacagcagaa gaacaggcag atgccctggc cggcttactg gattatttga atattaccag
     1861 cgcagttatt gtagccatat cagctggcgg gctttctctg tatccttttg ccattcgtca
     1921 cccgaaaaaa actaaagcaa tcattgcaat agatgctata gctggggaat atttaatgcc
     1981 tgaacaatcc aacaaattgg ctcaagccct ttttatgact aattttggtt tatgggtcgc
     2041 taaaaaaagc atgatttatt tccccaaatc atttatcaag aatttaatta aactggaagg
     2101 ttacctggat ccagtacgtg tagatgaccg ggtgaaggag atcttaaatt gtcgggagcg
     2161 cctggaaatc attgctgaac taatgaatag catggcggac tataaggctc gtaaaacagg
     2221 gaccgtgaat gacatggcag agggtgtaaa aaccagctgg tatgattttg ctaaaattaa
     2281 ctgccctgca ctaataattc atgggacaca tgatgctgat gtaaaatttt acaatggtgt
     2341 atttgcttat gagcgattag catcgaaaga aaaagagcgt ttctggattg aatacggcac
     2401 tcattttggg tttttctttg ctcgtcaggc gcgtgaggtg caacaaaaat tccagggatt
     2461 tataataaag catggttaaa cagtttcttg ttgaaaagtt catggcggag gaataaacaa
     2521 cttctaaatc tatctactaa ataacgtgag ttatagataa agaggatata aaaaccagtc
     2581 gaaccccagg ttacgtttgg gctgaggagg cgcataagca tcgtctcgaa gacttggcaa
     2641 aaaaacatta aatccgatac taggcttcga gacggcataa atgcctcctc agcccgagcg
     2701 cttcgagata ataaatgatt ttatcaataa ctcaagttaa atacattgtt ttaatgttat
     2761 aacaacaacg attggaacaa ataagactta aatcaagaaa cgtggtttgt gttgtatgaa
     2821 ttcagtcgtc aagagtttcc accttggagt cgctcatgac taaatgggaa attataaaat
     2881 atcattataa tttcccatac aattcatctt attaaattac tttacagcaa cttctttcca
     2941 ccaactggtt ttggtttctg caacccaatc ggcatttctt aaatttttaa caaacaaggc
     3001 agtttcctca gggcttgagt gcttggagtc aagttttata caccagtccc agtctccaga
     3061 ggtagaccaa agactttgaa caccattcat ctttgctatt gcagcagaat ctgaccaggc
     3121 atttttagtt tttaaaaaaa caacactgtt ccatgagttc attgtttctt ccttaatttt
     3181 aatcaattat gttttttcgc attgaacata aattcattgc gttatataaa attagactat
     3241 gaattatcag atttcaaact cgaatgaaaa ataattctga tattaggcct tttgcctaag
     3301 ctaattattt tgtttcacgg caaggcgaaa acgttttgag gaacagagtt tacatacaca
     3361 ggacttacga aaaactccaa
//
LOCUS       DASLOE010000237         3291 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.238, whole genome shotgun sequence.
ACCESSION   DASLOE010000237 DASLOE010000000
VERSION     DASLOE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.238"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            13..993
                     /locus_tag="U6309_002800"
     CDS             13..993
                     /locus_tag="U6309_002800"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEN5506210.1"
                     /translation="MNLKKAELHVHLEGTISPELAKKLAKRNQLTLPNGLIAPDEKSY
                     LSKDFLDFLKVYDTLASVIKNPQDYYDITFDYLKSNAQEHAIYIEMMYSPDHAEQSSG
                     IPSKEHLAAIQQAIDDAKSQFDIVGRIIITAVRHFGVEACERVAKQASIDKFPCVTGF
                     GLGGDEAKFPPQLFTKAYQIAADSGLECTVHAGEFDSAKGMEEAMKTLPIKRIGHGVR
                     VIDSPDIMAMVKDQGIALEVCPTSNIFLGLFKDMNSHPFPKLYEAGIKVSINSDDPPF
                     MSTTLAQEYKRVQKAYGYNNDTMNNITRMAIEAAFVDEKTRKELLTKLEQ"
     gene            1224..2582
                     /gene="gorA"
                     /locus_tag="U6309_002801"
     CDS             1224..2582
                     /gene="gorA"
                     /locus_tag="U6309_002801"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEN5506211.1"
                     /translation="MKTKHFDLIVLGGGSGGIASAVRAAQYGAKVAVIEQNHLGGTCV
                     NLGCVPKKIMYNASSIAETLHKSPDYGFFLENNAKLDWKRLVNKRNAYIERLRENYEK
                     RFSQHKITLIQGKGIFHDQSSITIDHTIYQAEHIIIATGSEPALPAINGIKHAIDSDG
                     FFSLTKLPAKVAVIGSGYIGVELAGILNSLGSETHLLMRGTRPLSRFDHMIGDTLMEI
                     MQKQGICIHQNHKAQAIHLHSDGRKSILCQSGSIIENIDVIISAVGRKPRTGNLNLDK
                     INVNMDDKGLILVDAFQNTSVKGIYAIGDVTNAPALTPVAIAAGRRLADRIFGNQPDA
                     CLNYDNICSVVFSHPPSGSVGLTEHEAIEKYGKNKIKIYQTRFIPMYDALSIDKTPTA
                     MKLVTLGKKEKIIGLHVVGYSADEMLQGFGVAIKMGACKKDFDNTIAIHPTSAEEFVT
                     MV"
     gene            2589..3125
                     /locus_tag="U6309_002802"
     CDS             2589..3125
                     /locus_tag="U6309_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEN5506212.1"
                     /translation="MNYSIRKFEDKYPELGERVYIDPQSTVIGNVTLGDDVSVWPMAV
                     IRGDVNYIQIGHSCSIQDGAVLHVTHDGPYTPGGRPLILGQGITVGHKALLHACTIND
                     YCLIGMGSIILDSAHIQKHVMIAAGSIVPPGKILQSGYLYLGSPVQAVRKLTAKEIEQ
                     IEYSAGHYIRLKNKYLTY"
BASE COUNT         1088 a          590 c          639 g          974 t
ORIGIN      
        1 ataggctata tcatgaattt aaaaaaggca gaattacacg tacatctaga aggaactatt
       61 tccccagaac tcgccaaaaa actagcaaaa agaaatcaat taactctccc taatggtctt
      121 attgcaccag atgaaaaaag ttacttatca aaagactttc tcgattttct taaggtttat
      181 gatacgctgg catccgtaat taaaaatcca caagattatt atgatattac ctttgattat
      241 cttaaatcga acgctcaaga acatgctatc tacattgaaa tgatgtactc tccggaccat
      301 gccgagcaat ccagtggaat tccttcaaaa gagcatttag cagcaattca acaggctatt
      361 gatgatgcta aaagtcaatt tgatattgtt ggacgtatta ttatcactgc agtacgccat
      421 tttggcgtag aagcatgcga acgcgtcgca aaacaggctt ctatagataa atttccttgt
      481 gtaacaggat ttggattagg aggagatgaa gcaaaatttc ctcctcaatt gtttaccaaa
      541 gcttatcaga tagcagcaga ctccggattg gaatgcaccg ttcatgccgg cgaatttgat
      601 tcagccaaag gaatggaaga agcaatgaaa actctgccaa ttaaaagaat tgggcatggg
      661 gttcgagtga ttgactcacc tgacattatg gccatggtta aagaccaggg tattgcattg
      721 gaagtgtgcc caaccagtaa catatttttg ggattattca aagacatgaa tagccaccct
      781 tttccaaagc tttatgaagc agggattaaa gtcagtatta attccgatga tcctcctttt
      841 atgagcacta cacttgctca agaatacaaa agagttcaaa aagcttatgg ctataacaat
      901 gacacaatga ataatattac gcgtatggct attgaagctg cttttgttga tgaaaaaacc
      961 cggaaagaac ttttaacaaa attagaacaa taatctcagt cttatattcc tgttgtcagt
     1021 actaagtccc tgctgacttg aggcgataac gatactgtta tatttttaca ggttggtgtt
     1081 gatacatggc ttaaacacca accataacta aaaccatctg gttgaaatta aaaaatattt
     1141 tagtttttta taaaattcag tttaatccct tggtagacca agtacgacct gctactatta
     1201 ttttattttc ctattaattg actatgaaaa caaaacattt tgaccttata gttcttggag
     1261 gaggaagtgg cggaatagct agtgctgttc gtgctgctca gtatggagcc aaagtagcag
     1321 ttattgaaca aaaccacttg ggagggacct gcgtgaattt aggatgtgtc cccaaaaaaa
     1381 ttatgtacaa tgcttcatca attgcagaaa cactccataa atctcctgat tacggttttt
     1441 tcctggaaaa taatgccaag ttagattgga agcgactagt gaataaacgc aatgcgtata
     1501 ttgaacgttt aagagaaaat tatgagaaac gattttcaca gcacaaaatt actctgattc
     1561 aaggtaaggg cattttccac gatcaaagtt ctatcacgat agatcatact atttatcaag
     1621 cagaacatat tattattgca actggcagtg agccagctct accagcaatt aatggcatta
     1681 agcatgctat tgattctgat ggtttctttt cattaaccaa attaccagct aaggtagctg
     1741 ttattggtag cggttacata ggtgttgaat tagcaggaat ccttaactct cttggtagtg
     1801 aaactcactt attaatgaga ggtacacggc cactgagtcg ctttgatcat atgattggcg
     1861 atactttaat ggaaattatg caaaaacaag gaatctgtat tcatcaaaac cataaagccc
     1921 aagctatcca tctccatagc gacggcagaa aaagtatttt gtgccaaagt ggctctatta
     1981 ttgaaaatat tgatgtcatt atatctgctg taggtcgaaa accgcggact ggcaatttaa
     2041 atcttgataa aataaacgtt aacatggatg acaaaggatt aatcctggtt gatgcattcc
     2101 aaaatacatc ggttaaaggt atttatgcta ttggcgatgt cactaatgct cccgcactaa
     2161 ctcccgttgc aatagcggca gggaggcgtt tggcagaccg aattttcggg aatcaacctg
     2221 atgcctgctt aaattatgat aatatttgtt cagtagtgtt ctctcatcct ccatctggtt
     2281 ctgtcggttt aaccgagcat gaagccattg aaaaatatgg taagaataaa attaaaatat
     2341 atcaaacccg tttcatccct atgtatgatg ccttgagtat tgataaaacc cctactgcga
     2401 tgaaattggt taccttgggt aaaaaggaaa agattattgg tttacatgtc gtcggataca
     2461 gcgccgatga aatgctacaa ggctttgggg ttgccataaa aatgggcgca tgtaaaaagg
     2521 acttcgataa tacaattgca attcatccaa ccagtgctga agaatttgta actatggtgt
     2581 aaatatacat gaactattcc attcgtaaat ttgaagacaa gtatcctgaa ttaggtgaac
     2641 gagtttatat tgatccgcaa tccactgtca ttggtaacgt aaccctggga gatgacgtat
     2701 ccgtatggcc tatggctgtt attcgtggtg atgtcaatta tattcaaata ggccattcct
     2761 gcagtattca agacggtgct gtgctgcatg ttactcatga tggtccttat actccaggcg
     2821 gtaggccatt aattttaggg caaggaatca cggtagggca caaagctcta ttacacgcct
     2881 gcactatcaa tgattactgt ttaattggca tgggatctat aattttagac tcagcgcaca
     2941 tacaaaaaca tgtcatgatt gctgcaggaa gcattgtacc tccgggaaaa atactccaaa
     3001 gtggatattt gtatttggga agcccggttc aagccgtcag aaaattaact gcaaaagaga
     3061 tagaacaaat tgaatattct gcagggcatt atataaggtt aaagaataag tatcttacat
     3121 attaggttac gagctactca tttttcttat ccacatatta cttttgataa attaaaaaaa
     3181 gaaatcaagc catttaaact caacttatga cttaaagagc cagtgaagta acgagtattt
     3241 gatgtagaaa agacgatgta atttatttta taaataaaaa aagcggcttg a
//
LOCUS       DASLOE010000238         3263 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.239, whole genome shotgun sequence.
ACCESSION   DASLOE010000238 DASLOE010000000
VERSION     DASLOE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.239"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            71..523
                     /locus_tag="U6309_002803"
     CDS             71..523
                     /locus_tag="U6309_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506213.1"
                     /translation="MPPVEVIQSCKNNSPANKSVLMTDIEGIGYNKDSEPNCKNQFDT
                     EIDGKLYGSATCDDKPYLIIADHKVKVESANNYSTNPAIKPTTAIAQVAGWWKIVDSK
                     KQMYICIESPLSNTGTASNAYQYFIVADPFNGKKSNFDISFYFFEKKE"
     gene            complement(528..1607)
                     /locus_tag="U6309_002804"
     CDS             complement(528..1607)
                     /locus_tag="U6309_002804"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024571.1,HMM:NF025019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN5506214.1"
                     /translation="MDDDNILNAVKQDPVGFVNRIDKAIIDEIQRAPELLRAIKLSID
                     ENRQPGRFLLTGSANLLALPQIGDSLAGRMEILTLFPLSLAEIQRRENHFIKYALDQS
                     WPSHITHSEQSDIISQALTGGYPEMLTRPTFERRNAWAKSYIKAIVERDVKDISSIEK
                     LVEMPRLLEVLAQQSGKLTNFTQIGGQLNLDTKTAQKYVGLLETLFLVHQLRPWHDNT
                     LNRIVKTPKIHFIDSGLLACLNRVTIESIEKDKSSFGALLETWVYSELLKMCTLANEP
                     WNIYYYRDKDQVEVDFILENHARKIIGIEVKASQTIVNQDFRGLRKLARLADKNWVSG
                     IVLYNGDKCLSFGDNLWAVPFTFLD"
     gene            complement(2239..2937)
                     /locus_tag="U6309_002805"
     CDS             complement(2239..2937)
                     /locus_tag="U6309_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506215.1"
                     /translation="MKFFNPNDKQESTFDKCMAEYRNLVFGNISERVNQLNEHAPNFK
                     PIDFEREQSVQVGVGECAFINADTEERLILGSVGAGTCFILAIVDPNNNSAWLAHIDV
                     LVSMPQLKEMLTKVFTKINPNSAVAHIVGGNDSSIVQGIAICNELEKNGVTISSALLR
                     SKMEMDQLSLAIDPKTGTIYAPEANNLKARNDLWRNLSNRMTKSNEVGSIGLTEILPL
                     PIESTSKHYSAPPI"
BASE COUNT          991 a          595 c          561 g         1116 t
ORIGIN      
        1 cgaagcaatg tatataaaaa aaataagcat atttctagtt tgtttatcca tatacggagt
       61 atcacatgcg atgccacccg ttgaagttat tcaatcatgt aaaaacaata gtccagcaaa
      121 taaatccgtc cttatgacgg acattgaggg gattggttat aacaaagatt cagaacctaa
      181 ttgcaaaaat cagtttgata cggaaattga tggtaaactt tatggatcag caacatgtga
      241 tgataaaccc tacttaataa ttgcagatca taaggttaaa gtggaatcag ctaataatta
      301 ttccactaac ccagctatca aaccaacaac cgctattgct caggttgcag gctggtggaa
      361 gatagtggat tcaaaaaagc aaatgtatat atgtatagaa tccccattat ccaacacggg
      421 tactgcatcc aatgcctatc agtattttat tgttgcagat ccctttaatg gaaaaaaatc
      481 aaattttgac atctcatttt atttttttga gaaaaaagaa taattattta atcaagaaat
      541 gtgaaaggaa ctgcccagag attatctcca aatgataaac atttatctcc attatagaga
      601 actatcccgc taacccaatt tttatcagct aaacgagcca gttttctcaa accacggaaa
      661 tcttgattaa caatagtttg acttgcttta acttcaatac caataatctt gcgtgcatga
      721 ttttcaagaa taaaatccac ttccacttgg tctttgtcac gataataata aatattccat
      781 ggctcattag ctagagtgca cattttcagt aactcgctat atacccaagt ttctagtaaa
      841 gctccaaaag aagatttatc tttttcaatg ctttctatgg taactctgtt cagacatgct
      901 aaaagaccac tatcaataaa atgaattttg ggtgttttaa ctatcctgtt taaggtattg
      961 tcatgccaag gtcttaattg atgtactaaa aatagtgttt ccagtaaacc gacatacttc
     1021 tgcgctgttt tggtatctaa atttaattgc ccaccaattt gagtaaaatt ggttaatttt
     1081 cctgattgtt gagccagtac ttccagtaat cgaggcattt ccactaattt ttctattgat
     1141 gagatatctt ttacgtctcg ttctactatt gctttgatat aagatttagc ccaagcattt
     1201 cgtctttcaa aagttggtct cgttaacatt tcgggatatc ctccagtcag agcttgtgaa
     1261 ataatgtctg attgttctga atgtgttatg tgacttggcc aggattggtc taaggcatat
     1321 tttataaagt gattttctct acgttggatt tcagctagag aaagtggaaa taatgttaat
     1381 atttccattc gaccagccaa gctatctcca atttgaggta gtgcgagtaa attggctgat
     1441 ccagttaata aaaagcggcc gggctgtcta ttttcgtcaa ttgaaagttt tatcgcacgt
     1501 agtaattctg gagcgcgctg aatttcatct atgattgctt tatcgattcg attaacaaaa
     1561 cccacaggat cttgttttac tgcattaaga atattgtcat catccaatgt atagtagggt
     1621 aaagaaggcg agtattattt gactaaagtt gtttttcctg attgccgagg cccgttaatt
     1681 aaaacaacgg gtgtatcctg catggcaatt tccattcgac ttttaagttg ccttggaaac
     1741 ttatttgttt tcgtattcaa gaaggtgtcc taagtttcgt ctaatatgga ttatacgtcc
     1801 gtccaaaatg gattatatct ccgtctaata tggaatatag cctcgtccaa aatggaatgc
     1861 aaatgaattt tcttcaattt atttaatgct catttgcaaa ttcatcagga tctgaccctt
     1921 attccgcaat attccccacc ttaattgcaa acaatgggga cggctaaaac ctagtagtga
     1981 cgcgcctgcc ccaaatgtcc cctttgtccc tgagatattg ggtaacatgt ttatgttaat
     2041 ttatttttat aattaataag tgttgatcaa tctagtctgc tctcattcat tttcaagagc
     2101 acattactac tgatgtttag ggtgtttcgg gcaggaattt ttgcatcaaa ctgctggaaa
     2161 tgtagacgca gtaaacttgt ctatttaact attcgtccag atatatcgca tttaattata
     2221 actatataca ccactgtctc aaataggggg ggcactataa tgcttgctag ttgattcgat
     2281 tggaagtggt aaaatttctg taagtccgat tgagcccact tcattacttt tagtcattct
     2341 attgcttaga ttacgccata aatcattacg tgcttttaga ttattagctt ctggggcata
     2401 aatagtgccg gttttaggat ctatggctaa gcttaattga tccatttcca tttttgatct
     2461 tagtaatgct gagctaatcg ttaccccatt tttttctaat tcattacaaa ttgctatacc
     2521 ttgtactata gatgaatcat ttcctccaac tatatgcgct acagcagaat taggattaat
     2581 ttttgtaaat acttttgtta acatctcttt gagttgtggc atagaaacta aaacatctat
     2641 atgagcaagc catgcagagt tattgttggg atcaactatc gctagaataa aacaagttcc
     2701 tgcgcctaca gagcctaaga tcagtcgttc ttctgtatca gcgtttataa atgcacattc
     2761 gcccacacca acttgtactg attgttctct ttcaaaatca attggtttaa agtttggtgc
     2821 atgttcattt aattgattaa cgcgttcact tatattacca aatacaagat ttctgtattc
     2881 tgccatacat ttatcaaaag tgctttcctg cttatcatta gggttgaaaa atttcattgc
     2941 ttacctcgtt gtaagcttag gactgcttat ggtttatata gttcataggt aagggtttgt
     3001 ctactaacac agatagacaa aaaactgcca atcaaaagac tcctatgtcc actcaagaaa
     3061 tgaaagtatt caagttgcgt actaaagtgg acaatttacc ttaaaagaaa ttggtgatta
     3121 ttttggttta cattattcta gtataagcta gcgtaggctg tttgcttgca aacaggcttg
     3181 tgtttatcag gatctgaccc tgatgaatcc tctaatgact caaatgtccc ctttgtccct
     3241 gagatattgg acaacatgct taa
//
LOCUS       DASLOE010000239         3237 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.240, whole genome shotgun sequence.
ACCESSION   DASLOE010000239 DASLOE010000000
VERSION     DASLOE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.240"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..545)
                     /locus_tag="U6309_002806"
     CDS             complement(<1..545)
                     /locus_tag="U6309_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN5506216.1"
                     /translation="MIDNTVKIIKHKVGLLNLAEELGNVSKACKVMGLSRDTFYRYKS
                     AVESGGIDALFDQNRRKPNIKNRVDESVELAVKEYAIAFPAHGQQRTSNELRKQGIFV
                     SPSGVRSIWLRYELANFKDRLKALEAKVTSEGIILTEAQVSALEKKKFEDEACGEIET
                     AHPGYLGSQDTFYVGTLKGVGR"
     gene            complement(768..3134)
                     /locus_tag="U6309_002807"
     CDS             complement(768..3134)
                     /locus_tag="U6309_002807"
                     /inference="COORDINATES: protein motif:HMM:NF024206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEN5506217.1"
                     /translation="MLNKTDNTDLLPTIFNQYFLVDLFAQLPVNEFFALRLLSKNINQ
                     LFNNQLIIKLIHRYFTPIKFHVPLEQLDAKQLIKELIESFQDLDTLECFEDIDTLGVI
                     DTKKVLVWDLHLAIASGDEQKIRRLFDLIPFHYFSTNERSQNDRNYYLDFSLFAMAAC
                     QPLPIRQLIFDLLTRGLNDSTDTLVDKHGFSKFDYAAILNLVDVLECSTSDDMIVHNN
                     ISPLRLACRYGQLDAVQSIINKFEWITFPINALKEASTSGHLDVVRYLCEMRNRLKNF
                     QVDDLNESLLAACKNGHLEVVRHLLDLGASIEAKDNEGNTPFILACQRDHLAVAKLLI
                     TKGAKPNAKNRSMEHVLNVVLKPRYGVASNEMIKFLLNLDLDLSNQDNEHIIPLRRAF
                     QWGNIEIIDIISQKAPITPSDAQEILHSMAWPGYIINENVIDYLIQKGANPNENEASY
                     GRSPLIAAIINNNLKLIRALFNKGADLNKLHEETGLSPLHYACSNSNLETIELLLELG
                     ANPNIKDKNGNGVFLKLEDRRKDRGSILQALIKKGFNINEPSCYGETPLLRAYSSIIS
                     IFFDKKEDLVSILLDHNADLNVVCKNPFFKGQSLFALACQGSDFTMIKRIFKSNENVL
                     LSNKQHQFSEMIGKHYIQSPDMAPLLKHFDRINNQNIAPHRYIYNLLAQYSRSDCDSN
                     YSAMFARFFTGHWGNHFGDLVYETLKKTKANKPFASVKDVSIFFQELSQTSSRSQLAN
                     RNRNGGTLHSIISLANLLMDTEFDDLPLTKSQAVVRDIQEDLTNFNLR"
BASE COUNT          986 a          610 c          579 g         1062 t
ORIGIN      
        1 cggccgactc ctttaagcgt acctacataa aaagtatctt gagagccgag gtaaccaggg
       61 tgggctgttt caatttcacc acatgcttca tcctcaaatt tctttttctc caaagcgcta
      121 acttgcgcct ctgttaatat aatcccttca gaggttactt tggcctctaa tgcttttaaa
      181 cggtctttaa aattggcaag ttcataccgc aaccaaatac tacgaacacc actaggagaa
      241 acaaaaatac cttgtttacg aagctcgttg ctcgtgcgct gctgaccatg tgctggaaat
      301 gctatcgcgt attccttgac tgctagctcg actgattcat ctactctatt tttgatatta
      361 ggttttcttc ggttttgatc gaataaagca tcaatgcctc ccgattcaac cgccgactta
      421 tagcgataaa aagtatctct ggataaaccc attactttgc atgctttaga aacatttcct
      481 aattcttcag caaggtttaa taagccaact ttatgtttaa tgattttaac tgtattatct
      541 atcatgagag ttttcctctt ggttttgttt aaagttcgca cttctatcaa aaccggaaac
      601 tctcactttt tcaagtagtt gtgtcagatt aagtcgaaac taattcacct aatgctgctt
      661 tgagttaaat gtaggttggt gctgagcttg cgaagcccaa caagggagcg tagcgacctc
      721 ctttacgttg ggctgcactt cgtttagcac caacctactt ttttgattta ccttaggtta
      781 aaattagtca agtcctcttg aatatcgcga acaaccgctt gtgattttgt taagggcaaa
      841 tcatcaaatt cagtgtccat caacaaattc gctaaactaa taatactgtg caaagtacca
      901 ccattcctat ttcgattagc caattgcgat ctgcttgaag tttgcgacaa ttcttggaaa
      961 aaaatgctta catccttgac agaagcgaag ggtttattcg cctttgtctt tttcaacgtt
     1021 tcataaacaa gatcaccaaa atgattaccc cagtgcccag taaaaaatcg cgcaaacatt
     1081 gcactgtaat tagaatcgca atctgaacgg ctgtattgag caagtaaatt ataaatatag
     1141 cgatggggtg caatattttg attgttaatt ctatcaaaat gtttcaaaag cggagccata
     1201 tctggacttt gaatgtagtg tttaccaatc atctcagaga attgatgttg cttattgcta
     1261 agtaaaacat tttcattcga tttaaatatc cttttaatca tagtaaaatc ggacccctga
     1321 caagctaggg cgaataaaga ctgacctttg aaaaaaggat ttttgcaaac aacattaaga
     1381 tccgcattat ggtcaagaag aatagataca agatcttctt ttttatcgaa aaaaatagaa
     1441 ataatagaag agtaagcgcg cagtaatgga gtctcgccgt aacaagacgg ttcattaatg
     1501 ttaaatcctt tcttgatgag tgcttgtaat atagagcctc ggtcttttct acgatcttcg
     1561 agctttaaaa atacaccatt accattcttg tctttgatat tgggattagc cccgagttct
     1621 aataaaagct ctattgtctc taaattacta ttactgcagg cgtaatgtaa tgggcttagt
     1681 cctgtttctt catgcagctt atttaggtca gctcccttat taaatagtgc tctgatcagt
     1741 ttcaaattat tattaataat ggccgctata agaggactcc tgccatagga ggcctcgttt
     1801 tcgtttggat tggctccttt ttggattaaa tagtcgatga cattttcatt gatgatgtaa
     1861 ccaggccagg ccattgagtg aagaatctct tgtgcatctg atggagtaat cggtgctttt
     1921 tgcgaaatta tgtctataat ttcgatgttg ccccattgaa atgcacgccg taacggaatt
     1981 atatgttcat tatcttgatt ggataaatct aagtccaaat ttaacaaaaa tttgatcatt
     2041 tcgtttgagg ctactccata gcgtggtttt aaaaccacat taagaacatg ttccatagat
     2101 cggttctttg cattaggctt tgcacccttt gtaattaaaa gttttgcaac agctagatga
     2161 tctctttggc aagctaaaat aaagggggta ttcccctcat tatccttagc ttcaatactt
     2221 gctcctaggt ctaataaatg cctaactact tctaaatgac catttttaca ggctgctaaa
     2281 agagattcat ttaaatcatc aacttggaag ttcttcaatc tattcctcat ttcacataga
     2341 tatcgaacca catccaaatg tccactcgta ctagcttctt tgagtgcgtt gataggaaat
     2401 gttatccatt caaatttgtt gatgatgctt tggactgcat caagttgtcc atatcgacaa
     2461 gctaacctta gtggtgagat gttattatgg actatcatat catctgatgt actacactct
     2521 aaaacatcga caagattcaa aatggcggca taatcaaatt tagagaaacc atgtttatct
     2581 actaaggtgt cagtagagtc gtttaaaccc ctcgttagca aatcaaaaat gagttgccta
     2641 ataggaaggg gttggcatgc tgccatggca aataaggaga agtctagata gtaatttcta
     2701 tcattttgag atctttcatt ggtggaaaaa taatgaaatg gaatcaagtc aaaaagcctg
     2761 cgaatctttt gttcatcacc acttgctata gctagatgta aatcccaaac taaaaccttc
     2821 tttgtgtcaa taacgcccag tgtatcaata tcttcaaagc attctaatgt atccaaatct
     2881 tgaaagcttt ctataagctc tttaattagt tgtttagcat cgagttgttc taaaggaaca
     2941 tggaacttga taggtgtaaa ataacgatgg atcaatttta taatcagttg attgttaaac
     3001 agttgattga tgtttttgct tagcaggcga agcgcaaaaa attcattcac cggtaattgt
     3061 gcaaataagt caactaagaa atactggtta aaaattgttg gcaataaatc ggtattatca
     3121 gttttgttaa gcattttcta atctcaataa gttattaaat catgtccaaa ggacacaaac
     3181 gaataattgg gatttttgct cgacaactgt gcaaaaagtg tttgaatgat aggagtt
//
LOCUS       DASLOE010000240         3222 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.241, whole genome shotgun sequence.
ACCESSION   DASLOE010000240 DASLOE010000000
VERSION     DASLOE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.241"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..904
                     /locus_tag="U6309_002808"
     CDS             <1..904
                     /locus_tag="U6309_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEN5506218.1"
                     /translation="QMLTEFYILKVTLKLVIVLLSVYLIGISWGFATDFIDTVYKKLL
                     YGFTLANITIYPTRILFGIIIFCFLYLVSRAISTSISRHQQFENEEETQVAIASILTY
                     VGFSIAVISGLLVAGFNFTGLAIIAGALSVGIGLGLQSIVNNFVSGIILLIEKPIRPG
                     DRINIDGVEGFVKKIRVRSTQILTPVNEDIIIPNSDLITRRVVNYMLTDNYWRVNCEV
                     SVAFGTNLNLVKDILLEIANKHDDVVKSGRNKPVVLFTSFADSALTFQLWCMIKDVNK
                     KSTVKSELNFSIEEAFRKHDISIT"
     gene            1077..3221
                     /locus_tag="U6309_002809"
     CDS             1077..3221
                     /locus_tag="U6309_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN5506219.1"
                     /translation="MIKKIHRWLPLLVLIGLLVLFFSLHLDKYLSFNALRENHALLIA
                     WTQTHYFIAPLIFIVFYTAAVAISIPGAVLFTLTGGFLFGVFWGVLFVVISATLGATI
                     LFFAVRTALGDWFAKKASGWIERMRRGFQHNAFSYLVTLRLIPLFPFWAVNIVPALLN
                     IRAKTFITATFIGIIPGTTVYVMVGNGLSQIFAANETPNLGIIFELQILGPLLALAVL
                     SLMPVLYQFSTRKHQNHHSFSDISRVINCDLAIIGGGAGGLSLASGCSQLGLKVVLVE
                     SGKMGGDCLNYGCIPSKSLLAAAKTFYYAKHATHFGVHTEAIKINFQQVMQHVHQIID
                     NISEHDSVQRFESLGVNVIKQVGKFLNHDTLQAGDSIIKAKRFVVATGSSPFIPPIPG
                     LDAVSYFTNETIFDLKEQPEHLIVIGGGPIGCELAQAFAMLGSKVTLLEGLNLLPKDD
                     PDCVAVLRTKMKSMNILIYEQIEITQINSHPDTGISVCFEFQNTQFTITASHLLIATG
                     RRANVKPLDLEKAGVKLTSKGVEVNKYLQTSNKKIYALGDVTGLYQFTHMASYQAGIV
                     LRNIVFKLPSKVDYRAIPWVTFTDPELAHVGLGVSDALKHPDAQIIEWPFVDNDRAQT
                     EHTLSGKIKIITDNKARILGVTIVGPHAGELILPWVMAIREKKTLRSFTDVIVPYPTL
                     SEISKRVAGSFYAPKLFSNKTRMLVRWLQKIG"
BASE COUNT          938 a          580 c          631 g         1073 t
ORIGIN      
        1 tcagatgctt actgagtttt atattcttaa agttacctta aaacttgtta ttgtactcct
       61 aagtgtctat ctgataggga tatcctgggg ttttgctact gattttattg atactgttta
      121 taagaaatta ttgtatggct ttactctggc aaatattaca atttatccaa cgcgcattct
      181 cttcgggatc ataatttttt gttttttata tttggtgtct cgcgccattt caacttcaat
      241 cagtcgtcac caacagttcg aaaatgaaga agaaactcag gtagctattg cttccatttt
      301 gacttatgtt ggatttagta ttgctgttat ttcaggctta ctcgtcgcgg gctttaattt
      361 tactggactt gccattatcg ctggagcact ttctgtagga attggtcttg gactgcaaag
      421 catagttaat aattttgtat caggtataat tttgcttatt gagaaaccaa taagacctgg
      481 agatcgcatt aatatcgatg gtgttgaagg atttgtaaaa aaaatcagag tcagatcaac
      541 acaaattctc actccggtaa atgaagatat tattattcca aactctgatt taattacacg
      601 ccgtgttgtc aattatatgc tgacagataa ttattggcgt gttaattgtg aggttagcgt
      661 ggctttcggg acaaacctga atcttgttaa agacatcctt ttggagatcg ctaataagca
      721 tgatgatgtt gtaaaaagtg gtagaaacaa acctgttgtg ttgtttacca gttttgccga
      781 cagtgcatta acttttcaac tctggtgcat gattaaagat gtaaataaaa aatcgacagt
      841 caaaagtgaa ttaaatttct ctattgagga ggcttttcgc aagcatgaca tttcaataac
      901 atagtcttaa tgtgattttc atatttgaat tgtagattaa atacttcccc tactctggaa
      961 aataatgctt agtatcatta aaccctatcg ataattaagg tatgacctca aagtaaattc
     1021 ctgttaggat cttcatataa cttatatcag cttatcgggt taaaggaata acataaatga
     1081 ttaaaaaaat acatcgttgg ttgcctttgc ttgtcttaat cgggctattg gtacttttct
     1141 tcagcctaca ccttgataaa tacttaagtt ttaatgcgtt aagagaaaat catgctttac
     1201 tcattgcgtg gacccaaact cattatttta ttgcgccact gatttttatt gttttttata
     1261 ccgctgctgt agccatctct attccaggag cagttctttt tactttaact ggaggatttt
     1321 tattcggggt attctggggc gtgttatttg tagtcatcag tgctacgctc ggggctacca
     1381 tactgttttt tgcggttcgc actgctttgg gcgactggtt tgccaaaaag gcatctggat
     1441 ggatagagcg tatgcgtcgg ggatttcagc ataatgcttt ttcttattta gttacactgc
     1501 gattaattcc attatttcct ttttgggcag ttaatattgt accggctctt ttaaatattc
     1561 gagcaaaaac attcattaca gcgactttta ttggcatcat tccaggaaca acagtatatg
     1621 taatggttgg aaatggttta agccaaatat ttgcagccaa tgagactcct aatcttggga
     1681 ttatatttga actgcaaata ttaggtccac tgctcgcgtt ggctgttctc tctctaatgc
     1741 cagttcttta tcaattctca actcgcaagc atcagaatca tcacagtttc tcagatatta
     1801 gtcgagtaat aaattgtgat ttagccatta taggtggtgg agctggaggt ttgagtttag
     1861 cttccggttg ttcacaatta ggactcaaag ttgttttggt ggagtcaggg aaaatgggag
     1921 gagactgttt aaattacggt tgtataccat ccaaatcctt acttgctgca gccaagacgt
     1981 tttattatgc aaaacatgca acacattttg gagtacatac tgaagcaata aaaattaatt
     2041 ttcaacaagt aatgcaacat gtgcatcaaa tcatagacaa catttctgaa cacgattctg
     2101 tccaacgttt tgagtcgcta ggagtaaatg ttattaaaca agttggtaaa tttttaaacc
     2161 atgatacctt acaagctggc gatagcatta ttaaagcgaa acgtttcgtg gttgcaacag
     2221 gttcatcacc atttatccca cccattcccg gattggatgc agtctcctac tttaccaatg
     2281 aaacgatttt tgatttaaag gaacaacctg agcacttgat tgtcatcggc ggtggcccta
     2341 ttggttgtga gcttgctcaa gcattcgcta tgctcggttc taaagttact ctgctcgaag
     2401 gattgaatct tcttcccaag gacgatcctg attgtgttgc tgtcctacgc acaaagatga
     2461 aatcgatgaa tatcctcatt tatgaacaaa tcgagattac ccaaataaac tctcaccctg
     2521 atacgggtat ttccgtatgt tttgaatttc aaaacacaca attcacaatt acagccagtc
     2581 atctgctcat tgcaactgga cgtcgggcga atgtaaagcc ccttgatttg gaaaaagcag
     2641 gggttaaatt aaccagcaaa ggtgtcgaag taaataaata cttacaaacc agtaataaaa
     2701 aaatctatgc tctgggtgat gtaacagggc tttatcagtt tactcatatg gcgagttatc
     2761 aggccggtat agtgcttcgt aatattgttt ttaaactacc gtctaaagtg gattatagag
     2821 caattccgtg ggttactttt actgatcccg aattagctca tgttggacta ggagtcagtg
     2881 atgctttgaa gcatcctgat gcccaaataa tcgagtggcc atttgtcgat aatgatcgag
     2941 cacaaactga gcatacatta agtggaaaaa taaaaatcat cacggacaac aaagcgagga
     3001 tccttggagt caccatagtt gggccacatg ctggagaact cattttacct tgggttatgg
     3061 cgattcgtga gaaaaaaacc cttcgaagtt ttacagatgt aatcgttcct taccctactc
     3121 tgagtgaaat cagcaaacgg gttgctggta gtttttatgc acctaaatta ttttccaata
     3181 aaacacgaat gttagttcgc tggttgcaaa aaattggata ag
//
LOCUS       DASLOE010000241         3201 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.242, whole genome shotgun sequence.
ACCESSION   DASLOE010000241 DASLOE010000000
VERSION     DASLOE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.242"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            375..1175
                     /gene="legP"
                     /locus_tag="U6309_002810"
     CDS             375..1175
                     /gene="legP"
                     /locus_tag="U6309_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector LegP"
                     /protein_id="HEN5506220.1"
                     /translation="MKTKSWIASLFYSSLCFSSSYAINLHQITITDPIMGSRTITYEQ
                     VGEFAVVEGDILIGKAENLKNQGAVITPKVGGSRWPNGVIPYEIAENLPFINKLAILQ
                     AIDHWQKNTNLEFVELTSKNRYDYRDYISFIPAEGTTCSSYVGRQGGKQEINLAPRCT
                     TMNTVHEIGHALGMWHEQSRSDRNQYIRIVWENIDEEHKFNFDQHLTDGKDFGEYDYG
                     SIMHYGRFAFSKNGQPTIIPLVDGVEIGQRTHLSEKDIAAVKAMYPET"
     gene            complement(1172..3010)
                     /locus_tag="U6309_002811"
     CDS             complement(1172..3010)
                     /locus_tag="U6309_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg3000 family Dot/Icm T4SS effector"
                     /protein_id="HEN5506221.1"
                     /translation="MVYSKQSAAFRRDKQQFFAQIPKDSKLDTKIVTIDDRVNTIDYT
                     FLLHHKQDLEAEFTQMFTVLQQQDDENKEIFWLYCYYCASLLEAFHKAYSQNSKQAEY
                     AKIKQKIKDHLNKVNVKEEESGFIQSLYNSFLGGFRNLISSPLHASKIRDYVAYSNLC
                     RIYWTFCRLTLTNGLSVARDLKIIEKLDAILGTHTDVDKIISTLQAPTGVITYFSVGF
                     FLMRLIIDGGMLLRHTFFPTELEKGAENGCEIHQMDYLPGPASLEAYRKSYILVHEDN
                     QEVSLYYIPRSGKAEKLSIKENNKLKDALLKKMNKDTSSLRLNSTEVEEIITHQTGHV
                     PEVTTAWDRFKHEFYKRHCNFANDFVWATINLLTNYNYLFNISAPAAGMITSVFLGFD
                     VCMALYKCNLAKQEYLIKKAQYLQEIEDYNNPEKFKKMTPEQRLLHIEMLNKQLRELE
                     INYKTQEATFYFVAAAAALLMMGFTASMLVSPPILVVGCFFACTLAVAMYLSTGSYSK
                     YEEKSLYLEEAQLTGKNLPVALKEYEAARNDFIFTMVKNTVMPIVLITTFAICWPAAI
                     VLTAMYIGYEIFHAYDQHSEAKAAKQLALAAPSEEKDGYMALATPN"
BASE COUNT         1025 a          576 c          562 g         1038 t
ORIGIN      
        1 ttctgcataa ttttctctta gcatggatat tgctttacaa gctattgttc atgaaactaa
       61 ggatattaat aattattcga ataaccagat aagcatttaa aattctttaa tcaaaatgga
      121 gatagtgctc aggttaacaa cattacaccg actcttggca aaaatattta aacttaacct
      181 tatatatttt gcaccgctat attcttatag ctatttattc ttgtaattgc gcataattat
      241 caattaagga cgttttgtaa aagcgttctt gctttaaggt tatcaagagt caacaaacta
      301 tataaaaagt tctggaaatc tactccctct ttcccctaaa ctgtccttgg tttttttaga
      361 cttaaggact atatatgaaa actaaatcat ggattgccag tttgttttat tcctcattat
      421 gtttctcctc ttcttacgca attaatctcc atcaaatcac aataactgat ccaataatgg
      481 gcagtcgtac cattacctat gaacaagttg gagaatttgc agtagtggaa ggtgatattt
      541 tgattggtaa agcagagaat cttaaaaacc aaggtgctgt tattacacca aaagtaggag
      601 gttcacgctg gccaaatggt gttatccctt atgaaattgc tgaaaattta ccttttataa
      661 acaagcttgc cattttacaa gcaatagatc attggcagaa aaacacgaat ttagaattcg
      721 ttgaattaac ttctaaaaat cgctatgact atcgtgatta catttctttt atcccggcag
      781 aaggcacaac ctgctcttcc tacgttggac gccagggagg gaagcaagag atcaatttgg
      841 cgccaagatg tactaccatg aatacggtac atgaaatagg acatgccttg ggcatgtggc
      901 atgagcaatc ccgatcagac agaaatcaat atattcgtat cgtctgggaa aatattgatg
      961 aagaacataa atttaatttt gaccaacatt taactgatgg gaaagatttc ggtgaatacg
     1021 actatggatc cattatgcat tatggtcgat ttgctttttc caagaatggt cagccaacta
     1081 tcatacctct agttgatgga gttgaaatag ggcagcgaac tcatttaagt gaaaaagata
     1141 tcgctgccgt caaagcaatg taccctgaaa cctaattggg agtggctaag gccatatatc
     1201 catccttttc ctctgaagga gcggctaaag ccaactgttt ggccgctttc gcttcagagt
     1261 gttggtcata agcatggaaa atttcataac caatgtacat agctgttaaa acaattgcag
     1321 caggccagca aattgcaaat gttgtaatta aaactatagg catgaccgta ttttttacca
     1381 tggtaaaaat aaaatcattc cgagctgctt catattcttt aagagcaact ggaaggtttt
     1441 tacctgtcaa ttgagcctct tccaaataca aacttttttc ctcgtatttc gaataagatc
     1501 ccgtagacag atacattgca acagcaagag tacaggcaaa aaaacaacca acgactaata
     1561 taggcgggct aaccagcatc gatgcagtaa atcccatcat caataaggcg gcagcagcgg
     1621 caacaaagta aaaagtagcc tcctgggttt tataattaat ttcaagctct ctaagttgct
     1681 tattgagcat ctctatatgc aataatcgct gttcaggagt catttttttg aatttttccg
     1741 gattattata gtcttctatt tcctgcaaat attgagcttt cttaatcaaa tattcttgtt
     1801 tggctaagtt gcacttatat aaagccatgc atacgtcgaa cccgagaaaa acagaagtga
     1861 tcatccctgc tgcaggagcg gaaatattaa aaaggtaatt atagttggta agcaggttga
     1921 ttgtagccca aacaaagtca ttggcaaaat tacagtgtcg tttataaaac tcatgtttaa
     1981 atctgtccca tgccgtagtc acttcgggaa catgccctgt ttgatgagtg attatctctt
     2041 ctacttcagt tgagttcaaa cgaagggatg atgtgtcctt attcattttc ttcagtaaag
     2101 catctttcag tttgttattt tctttaatgc ttaatttttc tgctttacca cttctaggaa
     2161 tgtaatagag tgatacttct tgattgtctt catgaacaag aatataactt tttctatatg
     2221 cttcgagact ggctgggcct ggcaaataat ccatttgatg gatttcacaa ccattttctg
     2281 cgcctttttc caattcagta gggaaaaagg tatgtcttaa tagcatacca ccatcaatta
     2341 ttaagcgcat caaaaagaaa ccaacactaa agtaagttat aaccccggta ggtgcttgaa
     2401 gagttgaaat aatcttatcg acatcagtat gagttcctaa aatagcatcc aacttctcaa
     2461 taatttttag gtctcgagct acgctcaatc cattagttaa agtcaaacga caaaacgtcc
     2521 aatagatacg acacaagttc gagtaggcaa cataatctct gattttggaa gcgtgcaatg
     2581 gagagctgat taaattgcga aaacctccaa gaaaggaatt gtacaaggat tgaataaaac
     2641 cagattcttc ctctttgaca ttgaccttat ttaaatggtc ttttattttt tgttttattt
     2701 tagcatattc agcttgttta ctgttttgtg aataagcttt gtggaaagcc tccagcaagg
     2761 acgcacaata gtagcaatac agccagaaaa tttctttatt ttcatcgtct tgctgctgta
     2821 acacagtaaa catttgagtg aattcagctt ctaaatcctg tttgtgatgt agtaaaaaag
     2881 tgtaatctat agtgtttact cgatcatcaa ttgttacaat ttttgtgtcc agtttactat
     2941 ccttcggaat ttgggcgaaa aattgttgtt tatccctacg aaatgcagca ctttgttttg
     3001 agtagaccat attaagaact cacaaaaatt acacaggaag ttttacaata atacgttacc
     3061 atttgatatg tattttgaca agcttttttg tattaatatt ttacacattg atttttgcga
     3121 ttcagactga tttttgcata aaaatgaatc aagataaaag ctggttcatt tgaaatgtgc
     3181 tgaaactaaa agaataaatt t
//
LOCUS       DASLOE010000242         3193 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.243, whole genome shotgun sequence.
ACCESSION   DASLOE010000242 DASLOE010000000
VERSION     DASLOE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.243"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            94..2970
                     /gene="rapA"
                     /locus_tag="U6309_002812"
     CDS             94..2970
                     /gene="rapA"
                     /locus_tag="U6309_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HEN5506222.1"
                     /translation="MTFRIGQRWISNTESQLGLGIITEVDGRQVSISFPAAGEDRIYA
                     IDNAPLSRIVYKTGEEILTNDQQRIKVTSIQEQDGVLLYTGNDDLGNVAQVSELALNC
                     FIKLNSPQQRLFSGLLDKLNAFKLRIKTLEHLGRLQQSKARGLLGSRTNHLIHQIYIA
                     YEVAQRFAPRVMLADEVGLGKTIEAGMILHYQLHTGRASRVLIIVPNSLIHQWFVEML
                     RRFNLHFSIIGPNQYDAMPSTLDELEENYNSIETASEDGNLFEDEQLVLTSLDFLMEN
                     ETARQQALAAQWDLLVVDEAHHLYWSEDSQSPEYTFIEKLSARGTGLLLLTATPEQVG
                     MKSHFARLRLLDPARFYDFSTFVNEEKQYHEINKIVQDLMDYKEKNSVDELNPQLHSH
                     LKQLLGKEVPAGINATIKELLDRFGTGRVLFRNTRAAIKGFPERRIHPVALTCPTLYS
                     EIIRESNLIHLYPETLLNNDSWIEHDPRVQWLVNKINELRPEKILVICAKAKTAISLE
                     QYLKLKTGIRSTLFHEGLTIIERDRAAAYFAEQENGAQVLVCSEIGSEGRNFQFSHHL
                     VLFDLPLNPDLLEQRIGRLDRIGQNHPIEIHVPYLAGTAQEKLFRWYHEGINILQQSC
                     SVGFSIFETFQNRLTPVLSNPMQETNEHSLEKLIADTQLHTHHIKESLNAGRDRLLEL
                     NSCNYAIGKELIEAIEAEENCLELENYMSQVYQEYGIDQEYHSENAEILRPGNHMKTA
                     HFPGLKEDGMTVTYSRPKALVREDMEFLSWEHPMVYDTMEMILESELGNATVTTISIK
                     SIKPGTLFLETFYTINSSAPKYLQLDRFIPSLPIRILMDTTGKNLSSILSYEQLNNLC
                     QPLKRHLGYSVIKQVREEIESILQQTNKIAENQMKLFIEEAKSMMELTTSQEVNRLES
                     LQKINPAIRDEEIEFFKKKISESNYFINSSTLKLQAIRVVINVS"
BASE COUNT         1033 a          650 c          601 g          909 t
ORIGIN      
        1 taggcatctc ctcagcccga acgaaccctg catatcgact gcttcttata taatccttat
       61 ccataattca tgttaatgag gacgtttctc tatatgacat ttcgcattgg tcaacgctgg
      121 ataagtaata ccgaatcaca actgggttta ggcattatta ctgaagtaga tggcagacaa
      181 gtttctatca gtttcccggc tgctggggag gatagaattt atgctataga taatgcgcca
      241 ttaagccgca tagtctataa aacaggtgaa gaaatactta ccaatgatca gcaaagaata
      301 aaagtaactt ccatccagga acaagatggt gttttactgt atacaggaaa tgacgacttg
      361 ggaaatgtgg ctcaagttag tgagctggca cttaattgtt ttatcaaact taactcccct
      421 caacaaaggc tcttcagcgg attattggac aaactaaatg cttttaaact cagaattaaa
      481 acattagagc atttaggccg attacaacag tcaaaagcaa gaggcctatt aggttcacga
      541 actaaccacc ttatccacca aatctatatc gcttatgaag ttgcccaaag atttgctccc
      601 agagtgatgc ttgccgacga ggttggattg ggaaaaacca ttgaagcggg aatgattttg
      661 cattatcaac tgcatactgg ccgtgcttca cgggtactga tcattgttcc aaattcatta
      721 attcaccaat ggtttgtcga aatgttaagg aggttcaatc tgcatttctc tatcattggt
      781 ccaaatcaat acgatgccat gccatccaca cttgatgagt tggaagagaa ctacaactct
      841 atcgagacag catcagaaga tggcaatctg tttgaagatg aacaactggt attaaccagc
      901 ctggatttcc ttatggaaaa tgaaacagct cgtcagcagg cattagctgc gcaatgggat
      961 cttctggtag tagatgaggc gcatcatctc tattggtcgg aagactcaca aagtcccgaa
     1021 tatacgttca ttgaaaaatt atccgctcga ggcacaggcc tgcttcttct tactgcgact
     1081 cctgaacaag ttggaatgaa gagtcatttc gctcgtcttc gtctacttga tccagcacgc
     1141 ttttatgatt tttcaacctt tgtaaatgaa gaaaagcaat accatgaaat taataaaata
     1201 gttcaggatc tcatggatta taaagaaaaa aatagcgtag atgagttaaa tccccaactc
     1261 cactctcatt taaaacaact cttgggtaaa gaagttcctg ctggcattaa tgcaacgatc
     1321 aaagagttgc ttgatagatt cggcacaggt cgagtacttt ttcgcaatac tcgtgccgcg
     1381 attaaaggat ttcccgaaag aagaatacac cctgtcgcat taacatgccc aacgctttat
     1441 tccgagataa tcagagaatc caacttaatc catctttacc ctgaaacttt gcttaataat
     1501 gactcctgga tagagcatga cccccgagtg caatggcttg ttaacaaaat taatgaatta
     1561 cgtccggaaa aaattctggt aatctgcgca aaggcaaaaa cagccattag tctggaacag
     1621 tatttaaaat taaaaacagg cattcgaagt actttatttc atgaaggact taccattata
     1681 gagcgtgaca gagctgccgc ttattttgca gaacaagaaa atggtgcaca ggttcttgtt
     1741 tgttccgaga taggaagtga gggaagaaac ttccagtttt cccatcattt ggttttgttt
     1801 gatttgccat taaaccctga ccttttggaa cagcgtatcg ggcgtttgga tcgtattggt
     1861 caaaatcatc ccattgaaat tcatgtaccc tatttagcgg gtaccgcgca ggaaaagcta
     1921 tttcgctggt atcacgaagg aatcaacatt cttcaacaaa gctgctctgt tggcttttca
     1981 atttttgaaa cttttcaaaa ccggctgaca cctgttttat ctaatcccat gcaggaaacg
     2041 aacgaacact cattagagaa gcttattgct gatacccaat tacacaccca ccatatcaag
     2101 gaatccctta atgcgggaag agatagatta ttagaattaa actcttgtaa ctacgctata
     2161 gggaaagaac tcattgaagc gatagaagca gaagaaaatt gccttgaact tgaaaattac
     2221 atgtcgcaag tgtaccagga atatggaatt gatcaggaat atcattccga gaatgccgaa
     2281 atattgcgcc ctggtaacca catgaaaacc gctcattttc ctggactaaa agaagacggt
     2341 atgaccgtca cttattctcg ccctaaagcc cttgttcggg aagacatgga gtttttaagt
     2401 tgggaacatc ccatggttta cgacactatg gaaatgatcc tggagtcaga acttggcaac
     2461 gccacagtga ctactatttc aatcaaaagc ataaaaccgg gtaccctctt cctggaaact
     2521 ttttatacca ttaattcttc cgcccctaaa tacttacaac ttgaccgatt tattccttca
     2581 ttgccaattc gaattttgat ggatactacc ggtaaaaatt tatcaagcat tttaagttat
     2641 gagcagctta ataatctgtg ccaaccactt aaacgtcatc tgggttactc tgttattaaa
     2701 caagttcgtg aagagattga atcaattctg caacaaacca acaaaatcgc agaaaatcaa
     2761 atgaaattat ttattgaaga agcaaaatca atgatggagc taacgacttc tcaagaagtt
     2821 aatcgactgg aatccttgca aaaaattaat cccgccattc gcgatgaaga aattgagttc
     2881 tttaaaaaga aaatttcaga gagtaattac ttcattaata gctccacttt aaaacttcaa
     2941 gcgattcggg ttgtaatcaa tgtcagttag ttaatcatcc cttgacaaat tgacacgcga
     3001 ataaaagaaa ttgcttgtct tacaggctgg acgctgattt cacaaaatca acaactatta
     3061 gattattaac ggaaaatttc tgtttatagg cctgtcgcaa caggattgac gccaatatta
     3121 ggattaatcc cacaatcatc cagaattttc aataaattat ttcctttacg ggttaatcgt
     3181 ttaatacagt att
//
LOCUS       DASLOE010000243         3175 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.244, whole genome shotgun sequence.
ACCESSION   DASLOE010000243 DASLOE010000000
VERSION     DASLOE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.244"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            44..598
                     /locus_tag="U6309_002813"
     CDS             44..598
                     /locus_tag="U6309_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN5506223.1"
                     /translation="MQRIKKISIANAQGKAKELLEGTKKAMGTELNLFSTLANSPAAL
                     EAYIGIMTSLSKGALNPKLREQIALVSAGYNGCNYCASAHTYLGEKAGINKDELKENL
                     SGKSSDKKTQVALNFATQLIERRGGVSEFDIKTVREAGFSDEEMVEILAHVAMNTFTN
                     YFNEAFKTDIDFPVVDTHKGRTAT"
     gene            1032..1232
                     /locus_tag="U6309_002814"
     CDS             1032..1232
                     /locus_tag="U6309_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEN5506224.1"
                     /translation="MIKELNRIVSNQTFDEQPLTALNSEAIDFRAALEQFEKIRKIDK
                     KDLETVVILMDYQGKKVPTVQG"
     gene            1326..1709
                     /locus_tag="U6309_002815"
     CDS             1326..1709
                     /locus_tag="U6309_002815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506225.1"
                     /translation="MLNFIILLEKQLKKQALLLISFAFNKAILTKQPDAKIVIPPPSV
                     AVISWKANTQRDDHIRLLQDEGDMVWQKKNNYGLRSHIELAILRYKKVMGTAMKAREL
                     PQQKTECGIATRALNESLHWVCQSL"
     gene            complement(1763..2716)
                     /locus_tag="U6309_002816"
     CDS             complement(1763..2716)
                     /locus_tag="U6309_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEN5506226.1"
                     /translation="MMRTTTGYFFMAEKSYTKPALTVSQQLEFLFSQGLQIEDQKLAI
                     RALETVSYYRLSSYLLPFKQSHNAKNPRQFKENATFEQVWQLYQFDRELRLLVADAIE
                     KIEVAFRAALTNVTSIRFNPFWYVERNYFKAKAGANRERDFFDDYLKTIKTISTNKQE
                     LFIQHYHKNYDKPYFPPIWMMVEALSFGVCSKMFNNIQSKDVRNEIASFLGQHTTVIE
                     SWIKSLTYTRNLCAHHSRLWNRWLVIPPLIPKNAPIKTHIDGNYRFQLIAFIIDQLLE
                     TIAPESNWKIKLFELFERNEQFSGIEMGFINNWRDDPIWHC"
BASE COUNT         1046 a          605 c          600 g          924 t
ORIGIN      
        1 atactgtcgt attccaatct atccaaaaat aaggagaagt atcgtgcaac gtattaaaaa
       61 aatctctatc gctaacgcac aaggcaaagc taaagagcta ctcgaaggca caaaaaaagc
      121 tatgggcact gaattaaatc tattcagcac acttgccaac tcaccagcgg cactggaagc
      181 ctacattggg attatgacct ctcttagcaa aggtgctctt aaccctaaac tacgcgaaca
      241 aatcgcattg gtctcagcag gttataatgg ctgtaactac tgcgcatccg ctcataccta
      301 tcttggagaa aaagcaggga ttaataaaga tgaacttaaa gaaaacctct caggaaaatc
      361 gtctgataag aaaacacaag tggctctcaa ttttgctact caactgattg aacgtcgtgg
      421 tggggtgagt gagttcgaca tcaagactgt ccgtgaagct ggatttagtg atgaagaaat
      481 ggttgaaatt ttagcgcatg tagcgatgaa cactttcact aactacttca acgaagcatt
      541 caagacggac atcgacttcc ctgtcgttga tacccataaa ggacgtactg caacataaat
      601 tattctaagg ggtggcttaa aaaccacctc cttaatagaa aatccaaatt atattttgcc
      661 ccaaatacaa accaccctaa gattcaatcc tcaagttttt attcttgact ttatagaacg
      721 tcgatttaca gcggttgaac tggaagatcc agagatctcg aaataccaat tgactcaatg
      781 gacaagcttt ttggtatcta cgtttttatt ttagaacaaa aaacatcaga gaaaaagttg
      841 ccattcttat tagcgatcaa ttgccccaca catttttccc gaaatagata ctattctatg
      901 gcgggatatt tatcttttaa ttgttcgtgt ctttccaagc tagactagac atcattatta
      961 taaaaaaatc aggaccagaa gaaggagtat atattcgtgc tggaaccaca aatagaaaag
     1021 ctgacacaga aatgattaaa gagttaaatc gcatagtcag caatcaaact tttgatgagc
     1081 aacccttaac tgcattgaac tccgaagcga tagacttcag agctgcttta gagcaattcg
     1141 aaaaaattag gaagatcgat aagaaagatc tagaaacggt cgttatctta atggattatc
     1201 aaggcaaaaa agtacctaca gttcagggtt aatattattt ggtaaggaca gagccaaaat
     1261 atttccagat gcatggattc aagcgggtcg atttcaagga acagacgaga gctttattgt
     1321 agataatgct gaatttcata attctcctgg aaaagcaatt aaagaagcaa gctttgcttt
     1381 taataagctt tgcttttaat aaggctatac tgacgaaaca gcctgatgca aagatcgtca
     1441 ttcctcctcc atcagttgca gtgattagct ggaaagcgaa cactcagcgc gacgatcata
     1501 ttcgcctttt gcaggacgag ggggatatgg tatggcaaaa gaaaaacaat tatggtttgc
     1561 gcagccatat agagttagct attttgcgtt acaaaaaagt catggggaca gccatgaaag
     1621 caagggaatt acctcagcaa aaaacagaat gtgggattgc tacgcgtgct ttaaacgaat
     1681 cacttcactg ggtatgccag tctctgtgaa agtgaagtaa aaccaattcg gttaggtgaa
     1741 atgttgccta attttgattt attcaacaat gccatattgg atcatcccgc caattattta
     1801 taaatcccat ttcaattcct gaaaactgct catttctttc aaatagctca aagagcttta
     1861 ttttccaatt agattcaggt gcaattgttt ctaataattg atctatgata aaagcgatta
     1921 attgaaaacg atagttacca tcaatatggg ttttaatagg cgcattctta ggtattagcg
     1981 gaggaataac aagccatcta ttccataatc ttgaatgatg agcacagagg ttccttgtat
     2041 aggttaaact ttttatccaa gattcaatta cagttgtatg ctgacctaaa aaagaagcaa
     2101 tctcattacg cacatctttc gactggatat tattaaacat cttagaacag acaccaaagg
     2161 agagtgcctc aaccatcatc caaattggtg gaaaatatgg tttatcataa ttcttatgat
     2221 aatgttgaat aaatagttcc tgcttattgg tactaatcgt tttaattgtt tttagataat
     2281 catcaaaaaa atctctttct ctatttgcac cagctttagc cttaaaataa tttcgttcta
     2341 cataccaaaa aggattaaat cggatacttg tcacattggt taatgccgct cgaaatgcca
     2401 cctctatttt ttcaattgca tcagcaacaa gtaaccttag ctctctgtca aattgataaa
     2461 gctgccaaac ttgttcaaat gtagcgtttt ctttaaactg gcgagggttc tttgcattgt
     2521 gggattgctt aaaaggcaaa agataggatg ataagcgata ataacttact gtttccaatg
     2581 cacgaatagc tagtttttga tcctcaatct gcaaaccctg agaaaataaa aactccagct
     2641 gttgactaac agtaagtgca ggtttcgtat aactcttttc agccataaaa aaataacccg
     2701 tcgtggtgcg catcattgag aggcgtgacg ggcatggtta attatattat agcgcaacca
     2761 ctctcaaatt caaatacgcg ttttgtattt ttattaactc aggtaataag agattatacc
     2821 attcagagtc agtctaatca aaatactaat aatcaacaat aggactctaa tgtggctgta
     2881 tgtcgcacag ggaaatattg attgggactg cctagcgctg cctgacacaa cagtgacgaa
     2941 atcgtgccgt aacagtgacc gtttgtagtg tatttgtggc agttctaggc agtaattcga
     3001 tgatttgatg catcgtaagt tgttgattta taatgttttc tattattgag tatcaggctt
     3061 cgaaccaagg tgtcgggggt tcgagtccct ccgagcgcgc cacttttagg gctcttcccc
     3121 aaatccgggg gcactaatca agaacacaaa actttaaccc ttaacctata gtttt
//
LOCUS       DASLOE010000244         3170 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.245, whole genome shotgun sequence.
ACCESSION   DASLOE010000244 DASLOE010000000
VERSION     DASLOE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.245"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            7..1410
                     /locus_tag="U6309_002817"
     CDS             7..1410
                     /locus_tag="U6309_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506227.1"
                     /translation="MIEVNIWLSTAFLFKKRIKHKFFGPLLASEDKGENVGHVNFTIE
                     IDERTKNSFDFIEQHGPELGAKKTLRVVPIKATEPSITNIESSHLKPTMVKSVVVSHS
                     FWPEGRPSKSETIKGKGVKPKFKTHEDDMIAEDSITTMDIIHRKSALEEIGKEKMENL
                     DLLVEISDLEVNMEQRLLFMDDLKKLHLEKEELSKKQKQLTSSHQTQLKDLKGQLAKL
                     ELHLNKTNGQIKATERTVSYLNKLENPDPKTRAQSIELTEKLIKLKKELETTISSQHE
                     ISELMSKSELTYHEGMQKIEKNLQQVELAINHREENLRQLDIKINGRDENDLKELQEE
                     ARHRKEYISRKEQFIKSRDFTKGRHPDYSVTLPTAESGFTYYVDEVKIVKAMQEERSQ
                     NYSFLFNNCASSAKRCLLAGIDEKLRAKLKEAGLDSKFFEISKVETCKSLRDWARTLE
                     SKLTELNFPLPAPTRGA"
     gene            complement(1476..1868)
                     /gene="erpA"
                     /locus_tag="U6309_002818"
     CDS             complement(1476..1868)
                     /gene="erpA"
                     /locus_tag="U6309_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEN5506228.1"
                     /translation="MASVDVSQNTSDIHFSVSAADKVAELIKEEDNSNLNLRVSITGG
                     GCSGFQYGFSFDEQINDDDTIIIQQCSDGKSSVKLLIDSMSYQYLHDAEIDYIKGIQG
                     EQFVIRNPNAKTTCGCGSSFSIGDEDDL"
     gene            complement(2003..3088)
                     /gene="mnmA"
                     /locus_tag="U6309_002819"
     CDS             complement(2003..3088)
                     /gene="mnmA"
                     /locus_tag="U6309_002819"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEN5506229.1"
                     /translation="MKAKVIVGMSGGVDSSVAAWLLKEQGYQVEGLFMKNWEQDDHND
                     YCPAAKDLADAQAVCNQLRIPLHTVNFSKEYWDRVFAYFLSEYEKGRTPNPDVLCNKE
                     IKFNAFLNHALTLGADYIATGHYAKNTIEGSIGYLFKAKDREKDQTYFLHAVEPEALS
                     KTIFPIGDFTKPQIREFAKQLGLVTHAKKDSTGICFIGEKRFKTFLNEFILAKPGEIK
                     STGGKTLGQHDGLMFYTLGQRQGLGIGGLQNSTDEPWYVVDKDIASNTLYVAQGSQHP
                     MLYSQGLICGPIHWLADYKNHLPLTCFAKTRYRQTDQACMISPPDNNLHYVMFSSPQR
                     AITPGQFIVFYEKNQCLGGATIEQIIR"
BASE COUNT         1050 a          559 c          596 g          965 t
ORIGIN      
        1 agcgttatga ttgaagtaaa tatttggtta tcaactgctt ttttgtttaa gaaacgaatt
       61 aagcacaaat tttttggccc gcttttagct tcagaggaca aaggggaaaa tgtagggcat
      121 gtcaatttta ctatagaaat tgatgagcgc acaaaaaata gttttgattt tattgaacaa
      181 catgggccag agctgggggc taaaaaaaca ttgcgtgtcg tcccaattaa agcgactgag
      241 ccttccataa caaatataga atcgtctcat ttgaaaccaa ctatggtaaa gagtgttgtg
      301 gtttcgcata gtttctggcc agaagggaga ccaagtaaat cagaaactat aaaaggtaaa
      361 ggagtcaaac ccaagtttaa aactcatgaa gacgatatga ttgctgaaga ttcaataacg
      421 acaatggata taatacatcg caaatctgct ttagaagaga ttggtaagga aaagatggaa
      481 aatcttgact tgctggttga aatttcagat ttggaagtca atatggaaca aagattactt
      541 tttatggatg atcttaagaa attgcatttg gaaaaagagg agctgagtaa gaaacagaag
      601 caattaacca gctctcacca aacccaatta aaagacctaa aggggcaatt agccaagctg
      661 gaacttcatt tgaataaaac aaacggacaa attaaagcta cagagagaac tgtatcctat
      721 ttgaacaagt tggaaaatcc tgatcctaaa accagagcac aatcgattga gcttactgaa
      781 aaattaatta aattaaaaaa agagctcgaa actacaataa gttctcaaca tgagataagc
      841 gaattgatgt caaaatctga actcacttat catgaaggta tgcaaaaaat tgaaaaaaat
      901 cttcaacagg ttgagcttgc aattaaccac cgtgaagaaa atctaaggca attggatatt
      961 aaaatcaatg gcagggatga aaatgattta aaggaattac aagaagaggc tcgccatcgt
     1021 aaagaatata tttctcgtaa agaacaattt atcaaaagcc gtgattttac taaaggtaga
     1081 catcctgatt acagtgtcac tttgccaact gcggaaagtg gttttactta ttatgttgat
     1141 gaagtcaaaa tagtgaaagc tatgcaagaa gaacgcagcc aaaattactc atttcttttt
     1201 aataactgtg ccagtagtgc taaacgttgt ttgctagcag gaatagatga aaaattgcga
     1261 gccaaattaa aagaagctgg tttagatagc aagttttttg aaattagtaa agtggaaaca
     1321 tgcaagtcat tacgtgactg ggcaagaact cttgagagta aattaacgga acttaatttt
     1381 ccattgccag ctcctacccg aggtgcttaa gaataataag cagttcgtcc gtaaaaaaac
     1441 taagatcact gcctgagttt taggcagtga tttttttata aatcatcttc atcaccaata
     1501 ctaaacgaag agccgcaacc acaagtcgtt tttgcattag gattgcgtat gacaaattgc
     1561 tctccttgaa ttcctttaat gtaatcaatt tccgcatcat gtagatattg gtaactcatt
     1621 gaatcaatta gcaatttgac agaagatttt ccatcagaac attgttgtat gataatcgtg
     1681 tcgtcatcat tgatttgttc atcaaagcta aatccatatt gaaatccaga acaaccacca
     1741 cctgtaattg agacgcgtaa attcaaattg gaattatctt cttctttaat taattctgct
     1801 actttatcag ctgcgctgac agaaaaatgg atatctgaag tgttttggga tacgtcaaca
     1861 ctggccatac ttagctccaa aataattact accattatta ttcattatat caataatgtg
     1921 agttgtttcg tagtctcaat ccataaattg gtatggggac cagagaaaac aaacagtacc
     1981 agttcaaaat ttgcttccct gtttagcgaa taatctgctc tatagtagcc ccacccaagc
     2041 attgattttt ttcataaaag acgatgaatt gtcccggagt gatcgcacgt tgaggacttg
     2101 aaaacatcac atagtgcaga ttattatccg gaggagaaat catgcaggct tgatcggttt
     2161 gcctatatct ggttttggca aaacaggtta gtggtaaatg attcttatag tctgccagcc
     2221 aatgaatggg accacatatc aagccttggg aatagagcat tggatgttga ctgccctgtg
     2281 ccacgtaaag ggtgttactg gctatgtctt tatctacgac ataccatggc tcatctgttg
     2341 agttttgtaa tcctccaatt cccaaaccct ggcgttgtcc taatgtgtaa aacattaaac
     2401 catcatgttg accaagtgtt tttcctccag tactttttat ttctcccggt ttggcaagta
     2461 tgaattcatt aaggaaagtt ttgaatcttt tttcacctat aaagcaaatt cctgtagaat
     2521 cctttttggc atgagtgaca aggccaagtt gtttagcgaa ttctctaatt tgtggcttgg
     2581 taaaatcacc tattggaaaa atggttttgg acaatgcctc tggttcaaca gcgtgaagga
     2641 aatacgtttg atccttttcc ctatcttttg ccttaaataa atagccaata gatccttcta
     2701 ttgtattttt ggcatagtgg ccagtagcta tataatctgc gccaagggtt aaagcatgat
     2761 tcaaaaaggc attaaatttg atttctttat tacataacac gtcggggttt ggcgttcttc
     2821 ccttttcata ttcactcaga aaataagcaa agactctatc ccagtattct tttgaaaaat
     2881 ttacagtatg tagtggtatg cgcaattgat tacagacggc ctgtgcatct gccaaatctt
     2941 ttgctgcagg gcaataatca ttgtgatcgt cttgctccca gtttttcatg aatagtcctt
     3001 ccacctggta tccttgttct ttcaatagcc aggctgctac agaagagtct actcctcctg
     3061 acataccaac aataacttta gctttcataa atggtttgta tcgctctttt ttgaatcata
     3121 atcatattgg aatgtgcgat tttaacaaat taactacact ctaggaaatt
//
LOCUS       DASLOE010000245         3147 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.246, whole genome shotgun sequence.
ACCESSION   DASLOE010000245 DASLOE010000000
VERSION     DASLOE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.246"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            210..1448
                     /locus_tag="U6309_002820"
     CDS             210..1448
                     /locus_tag="U6309_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEN5506230.1"
                     /translation="MNWKEKVKTKFYSCDRFVRFVIQHFIQDDCTYIASALAFTSLLA
                     VVPLMSVGLAIFSSFPVFQGLAEPVQNFIFDNFVPATGKIVQSYLQQFTSQVSKLSIW
                     GVVFLIFTALLVMFTIERAMNKIWRVSSSRHGVSAFLLYWAIISLAPVLLGLSLAASS
                     YLFSMPILADHRAPYTILHYSPFFLSLIGFTFLYVVVPNCPVKIRHAFWGGLVAAILF
                     ESAKHAFAYYLIRYNTYELLYGAFATVPIFFIWVYWVWIITLLGAEISYAFSVHHQRR
                     GGKSLDGFSHALLWLHQLWIAQQHGKGLSFNDLVDASKQPFAVDVDEMINALIYHELI
                     HATADGHYMLSRDLSHVTLYDLTQLLPYRLPTHLELQYSKASLSEQWRAAFKRHNEEL
                     KKSLDINLEELFKKTGTILK"
     gene            complement(1617..>3147)
                     /locus_tag="U6309_002821"
     CDS             complement(1617..>3147)
                     /locus_tag="U6309_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5617 domain-containing protein"
                     /protein_id="HEN5506231.1"
                     /translation="FPEVNANFSMTDISMGDLHANALLFLNILVRQGIIAISPENYAK
                     FAEIYILPELQADYWGTEAPVFSAGNKQERLEEIKKQYNALIAQIKIINTKKLIRLIG
                     DELVDRGVIDYFILKLLQALHDQGADFEILLSNHGIEFVEACELFKENGNKLVAKRLG
                     NIQHGNSFHALQEAIAAGSISNEEVLNIYHQVYKKHLKIISYALDPDANEIKVFSHAG
                     IGLNHIRGLTRKFKVPYSEESAVDLARTIDAINKKFAEKASAGEIHTLYTHDMMYRGY
                     AGEYLNSTDEVVAATVWGREYGDLIRTSKKFKVTFIHGHDSYDPEKVEHVTLNNQLGQ
                     FQNNVGDLYLYATNGMRAVPTQCLNPDKKVQSLREKNRPDKPNDYVVKVHHTKPSFFK
                     TAHPKMTFPDSYKRIWDSTPGHSNITKIKALLKDYTKEDSILGSFWGLIFTLHWGRHH
                     VKSVHQIAQTQYTSVEAILSDLKALKPREGGSLDKRIKFIESQIITQRGDNPDLQFNL
                     K"
BASE COUNT          901 a          614 c          551 g         1081 t
ORIGIN      
        1 actgtaaaga agtattatgt tattgctaaa ttgtatgatg atataaataa ccttttattc
       61 atttgatttg ctcatgagag tttagacaaa ctacatgacc gatttaaaaa cttttttaac
      121 aaaaagattt gagtttttcg taaatcctaa ctatacaaaa acgagcataa aacgatatag
      181 ttatacaata aaaatcttgt atttaatcca tgaattggaa agaaaaagta aaaactaaat
      241 tctactcctg cgatcgattt gttcggtttg ttatccaaca tttcatacaa gacgattgta
      301 cctatattgc ctcggcatta gcctttacaa gcttactggc agtcgtgcca ttgatgtctg
      361 tgggtttggc aatattttct tcatttccag tattccaagg tttggcagag ccggtacaga
      421 attttatttt tgacaatttc gttccggcca caggtaaaat tgtacaaagc tatttacaac
      481 aatttacttc ccaagtctca aaactctcaa tttggggtgt ggtctttcta atttttaccg
      541 cgttattggt catgtttact attgaacgag ccatgaataa aatctggaga gtgagttcat
      601 ctcggcatgg tgtctctgcc tttttactct attgggctat catttctctt gccccggttt
      661 tattaggttt gagcctggca gcaagctcct atttattctc catgccaata ttggcagatc
      721 atcgagctcc ttacactatt cttcactatt cccctttttt tctttcactc atcggtttta
      781 cctttttgta cgtggtggtt cccaattgtc ctgtcaaaat ccgtcatgct ttttggggtg
      841 gcttagttgc cgcaatcttg tttgaatcag ctaagcatgc atttgcttat tacctgatac
      901 gctacaatac ctatgagttg ttgtatgggg catttgccac agtacctata tttttcattt
      961 gggtttattg ggtatggatt attactttgt tgggcgctga aatcagttat gccttttcag
     1021 tgcatcatca gcgtcgtgga gggaagtccc tcgatggctt ttcacatgcg ttgctctggt
     1081 tgcaccaatt atggatagcg caacagcatg gaaaaggatt aagctttaat gatctggtgg
     1141 atgccagcaa acaacctttc gctgtggatg tggatgaaat gatcaatgct ctgatttatc
     1201 atgaactgat ccatgctacc gctgatgggc actacatgtt aagtcgtgat ctcagtcacg
     1261 ttactctcta tgatttaact caattattac cttatcgctt gccaactcat ctggagctgc
     1321 aatattccaa agcctctctc tctgaacaat ggagagccgc ctttaaaagg cataatgaag
     1381 aattgaaaaa atccctggat attaatttgg aagaattatt caagaaaaca ggaacaatac
     1441 tcaaatgagc acatgtaatg gtttcagctt attcaggtgg tctactaaac aattctctac
     1501 gactgacttt ctaagtcagt ggcatataca cagtgccctt gatacctcag acaagcagca
     1561 atacgcaagc gatttaacaa gtcatttaaa aattttaagc gaaaaaaaca aaaagactat
     1621 ttcaaattga attgtaaatc tggattatca cccctctgag ttataatttg tgactcaata
     1681 aacttaatgc gtttatccag tgaaccgccc tctcttggct tcagtgcttt caaatccgaa
     1741 agaatagctt ctactgatgt atattgggtt tgcgcaatct gatgcacgga tttgacgtga
     1801 tgtcttcccc agtgcaatgt aaatattaat ccccagaaag aacccaaaat actgtcttcc
     1861 ttggtataat ctttaagtaa tgccttaatt ttggttatat tggaatgtcc tggtgtgcta
     1921 tcccatattc gtttgtagct gtccgggaat gtcatcttgg gatgagctgt tttaaaaaat
     1981 gatggttttg tatgatggac ttttacaaca taatcattgg gtttatctgg tcgatttttt
     2041 tcccttaggc tctgaacttt tttatctgga ttcaaacatt gtgttggaac cgccctcatg
     2101 ccattggtgg catacagata taaatcacct acattatttt ggaattgccc tagttgatta
     2161 tttaaagtga catgttctac cttttcagga tcgtaactat cgtggccatg aataaaggtg
     2221 actttaaatt ttttagacgt tcgaatcaaa tccccatatt cacgtcccca tacggttgcg
     2281 gcaaccactt cgtctgttga attcaaatat tcacctgcat aacctctata catcatgtca
     2341 tgggtataaa gcgtgtgaat ttctcccgca gatgcttttt ctgcgaattt tttgttaatt
     2401 gcatcaattg ttctagccaa atcaacagcc gactcctctg aataaggtac tttaaatttt
     2461 ctggtaagtc ctcgaatatg atttaaacca ataccagcat gactaaacac tttaatttca
     2521 tttgcatctg gatccaatgc gtaagaaata attttcaagt gttttttgta aacttgatgg
     2581 taaatattta atacttcttc atttgaaatc gaaccagctg ctattgcttc ttgcaaagca
     2641 tgaaatgaat taccatgctg aatatttcct agtctttttg ctactaattt gtttccattt
     2701 tccttgaata gttcacaggc ttcgacaaac tcaatcccat gatttgaaag taatatttca
     2761 aaatcagcac cttgatcgtg caaagcttgt aataatttta aaatgaaata gtcaataaca
     2821 ccgcgatcta ctaattcatc acctatcaag cgaatcaatt ttttagtatt aataattttg
     2881 atttgcgcta ttaatgcatt gtattgtttc ttgatttcct ccaaacgctc ctgtttattt
     2941 ccagcgctaa aaacaggtgc ttcggtcccc cagtaatctg cctgaagctc tggcaaaata
     3001 taaatttcag caaatttggc ataattttcg ggagaaatag caattattcc ctggcgaaca
     3061 agaatattga ggaaaagcaa agcattagca tgcaaatctc ccatgctaat atcggtcatg
     3121 gaaaaatttg catttacttc aggaaac
//
LOCUS       DASLOE010000246         3103 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.247, whole genome shotgun sequence.
ACCESSION   DASLOE010000246 DASLOE010000000
VERSION     DASLOE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.247"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            71..1201
                     /locus_tag="U6309_002822"
     CDS             71..1201
                     /locus_tag="U6309_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEN5506232.1"
                     /translation="MKEKYIGLHKIELNTPCLVIDYEKLKYNLKVMQQHGFEWGIGIR
                     PHCKTHKCTQIARLQLEYGAIGISAAKLSEAEVLVSKQITNVLITSPIVSEAKYSRLF
                     NCLKSAPDLMLVVDGKDNIRMLNELGKSLNQPVYVLLDIDSGIGRTGINPEVALEYGH
                     FINSMNWLKLKGIQCYAGHLQHIQNYEERKAFSLRIMDMASRIVKSFRDANLPCEILT
                     GTGTGTFDIDVEASEVTEIQPGSYAVMDVEYGSIGSKNNPSSFQPFKNSMTLLTSVIS
                     SNRKEHATVDAGTKSIYVDPHHKPHIISHPGLVYDWGGFGDEHGKVFSCEGGSHFLQN
                     GDVLELIVPHCDPTINLYDKFYITENNVVVDIWDIDMRGKSQ"
     gene            complement(1242..1421)
                     /locus_tag="U6309_002823"
     CDS             complement(1242..1421)
                     /locus_tag="U6309_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEN5506233.1"
                     /translation="MLSWALIFFIIAIIAAAFGFGGIAVAAAGIAKILFFLFLVMFVI
                     FLIMGLLGRRGPPPV"
     gene            1512..1880
                     /locus_tag="U6309_002824"
     CDS             1512..1880
                     /locus_tag="U6309_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506234.1"
                     /translation="MQTTKAHYSKIFFALFLFFCTGSSALFACPCFNMGFLHVAFLDD
                     KDTECRIYKEGSVVYKIEIFDKEKIASSTHTQCTLNTEHHDVFMNYDFHYLDDHGDCI
                     REILDACNKLKINSLHMDDE"
     gene            complement(1973..2503)
                     /locus_tag="U6309_002825"
     CDS             complement(1973..2503)
                     /locus_tag="U6309_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506235.1"
                     /translation="MSKFPHKTPETLRQYFQAKDLGQLTEINHSYGPHFENLENAIDR
                     LTQEISTQKGKLSGFLKEQEKISQNYGKIVIEQERFENNRASIMSDSCTGAERYLALA
                     ALGMSPFDCYYSNSRSLQDQIDTTNKKLNELNSHFAFWKNQRSKAVSELKILNSIIDE
                     KRKELEVDHQFTLSSN"
     gene            complement(2724..3065)
                     /locus_tag="U6309_002826"
     CDS             complement(2724..3065)
                     /locus_tag="U6309_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506236.1"
                     /translation="MSMTLSLGQKYGSFFWGCTKFAVSPYTNTFLSIVKSKEVEEADC
                     LSKALAASIVCGVLTFVVPVLPVLCAFTFTLSSIAMLLAVASMFVTYPIAILGDALCG
                     PELSDDYHYSF"
BASE COUNT          999 a          529 c          599 g          976 t
ORIGIN      
        1 ataaaagctt aaattcctaa gcaatttatg tcataaaacg catttattga actttgtgat
       61 gaattatctc atgaaagaaa aatacatcgg gcttcataag atagaactaa ataccccatg
      121 ccttgtgatt gattatgaaa agctgaaata caacttaaaa gtgatgcaac aacatggttt
      181 cgaatggggc ataggaataa gaccgcattg taaaactcat aaatgtactc aaatcgcacg
      241 tttacaactt gaatatgggg caatagggat ttctgccgcc aaactatcag aggctgaagt
      301 attggtatcc aaacaaatca cgaatgtctt aattacttca cccattgtat cggaagccaa
      361 gtattcaaga ttatttaatt gtcttaaatc agcgcctgat ttaatgctgg ttgtagatgg
      421 caaggataat attcgcatgc ttaatgagct tggaaagtcg ctaaaccagc cagtttatgt
      481 cttgcttgat atcgattcag gcattggtcg aacgggcatt aatcccgagg tagctttgga
      541 atatggtcat tttatcaata gtatgaattg gcttaagcta aaaggaattc aatgttatgc
      601 aggccacttg caacacattc aaaactatga agagagaaaa gcgttttctc tacgtattat
      661 ggatatggcc agcaggattg ttaagagttt tagagatgcc aatctccctt gtgaaatatt
      721 aactggcacc ggaactggta cttttgatat tgatgtcgaa gcaagtgaag taacagaaat
      781 ccagcctggc tcatatgctg taatggatgt tgagtatggt tcaattggtt caaaaaataa
      841 cccatcaagc tttcagccat ttaaaaactc gatgactctt ttaacaagtg ttataagcag
      901 taatcgtaaa gagcacgcta ctgttgatgc tggaacgaaa tcgatttatg tcgatcctca
      961 tcataaacca catattatta gtcatcccgg actggtttat gattggggtg ggtttggaga
     1021 tgagcatggc aaggtatttt cttgtgaagg cggaagtcat ttcctgcaaa atggcgatgt
     1081 tttagagctt atcgttcctc attgtgatcc gacaataaac ttgtatgata agttttatat
     1141 cacagaaaat aatgttgttg tcgatatttg ggatatcgac atgcgtggaa aatcacaata
     1201 atctatacta tttaaccagg tgtttgagct tcatattttt gctagacagg aggagggcca
     1261 cgacgcccga gtaatcccat aataagaaat atgacaaaca tcaccaaaaa tagaaagaat
     1321 aatatttttg caattccagc agcagccact gcaatgccgc caaatccaaa cgcggctgca
     1381 ataattgcaa tgataaaaaa aataagtgcc caacttaaca taacagctcc ctgtaaagat
     1441 tatcctgttc attaaattat agactaataa tggtctacaa ttagacaaaa gacagttaat
     1501 cagggaagaa tatgcaaacg actaaagctc actatagcaa gatattcttt gccttatttc
     1561 tttttttctg cactggttca agcgcattat ttgcctgccc ttgttttaac atgggctttt
     1621 tacacgtagc ctttttggat gataaagata cggaatgtcg catatataaa gaaggaagtg
     1681 tcgtttataa gatcgagatt tttgataagg aaaaaattgc gtcaagtacc catacccaat
     1741 gtacgctgaa tacggagcat catgatgttt ttatgaatta tgattttcat tacctcgatg
     1801 atcatgggga ttgtatcaga gaaatattgg acgcatgtaa caaactgaaa ataaactctt
     1861 tgcatatgga tgatgagtaa gataaagcaa ggtgaaatag gactggtatt caatgattag
     1921 gacgctcgcc cggattataa ttaagattaa cccgggttag gcttatcaat ttttagtttg
     1981 aactcaatgt aaattgatga tcaacttcaa gctcttttct tttttcatcg ataatactat
     2041 ttaatatctt taattcagaa accgctttcg atctttgatt tttccaaaaa gcaaaatgag
     2101 aatttaattc atttaatttt ttgtttgtcg tatcgatttg atcttgtaaa ctcctgctat
     2161 tgctatagta gcaatcgaaa ggcgacattc caagtgcggc gagtgctaaa tagcgttctg
     2221 ctccggtaca agagtcgctc ataatcgatg cacgattgtt ttcaaatcgt tcttgttcaa
     2281 ttacgatttt tccataattt tgggaaatct tttcctgttc ttttaaaaat ccggatagtt
     2341 ttcctttttg agtggatatc tcttgagtca gtctatcaat cgcgttttcc agattttcaa
     2401 aatgaggccc ataggaatga ttaatttctg ttaattgccc cagatctttt gcttggaaat
     2461 attgacgaag tgtctcgggg gttttgtgag ggaatttaga catgttttta cctttttgaa
     2521 tcaaaatcag catatcttag catatgcttt ttgatcatat caaacatata tgctgcataa
     2581 tgagcaaatt aatggctttt gttgatggct gggttcattt tttctcaata tcaaacagga
     2641 agagtatttt ttacgagttt aaaaatactc ttcctgtttg ttgatactta tgatgatttc
     2701 aaatgaaatt atcttaaaac tgattagaaa gaataatggt aatcgtcaga caattctggt
     2761 ccgcacagtg catcaccaag tattgcaatc ggataagtca cgaacatgct ggctaccgca
     2821 agcaacatgg ctattgaaga cagggtaaac gtaaaagcgc atagaactgg taataccgga
     2881 acaacaaaag tgagaactcc gcagacaatg ctggcagcaa gagccttgga taaacaatct
     2941 gcttcttcaa cctctttcga tttgacgatg gatagaaaag tattggtata gggggaaaca
     3001 gcaaatttag tacatcccca aaagaatgag ccgtattttt gacctaacga gagagtcatt
     3061 gacatttttt actccaattt aacttaaatt aggtcagcaa tta
//
LOCUS       DASLOE010000247         3065 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.248, whole genome shotgun sequence.
ACCESSION   DASLOE010000247 DASLOE010000000
VERSION     DASLOE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.248"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..551
                     /locus_tag="U6309_002827"
     CDS             <1..551
                     /locus_tag="U6309_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506237.1"
                     /translation="NKTFITDGLATCAAVAIYGSDSGCSAAFTHMSSEASDKDDKRKE
                     TILNEMLEYVLKSNALSDVKLIISPSRIQEKHLISFILEWVKQKKIACSMLETGGDSA
                     VFNIDKDGRALMLSTSLQLKQDASNKTWHGHGIIIGQSEGIPVIEKQKKHFVFFAYDI
                     SFQITKLSDPPKTVGLTMAVSA"
     gene            685..1110
                     /locus_tag="U6309_002828"
     CDS             685..1110
                     /locus_tag="U6309_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506238.1"
                     /translation="MRRHFIIIVLGIFQFGCIPLAEHHNKISPATTHEEYHDVNFSNL
                     YEKNRSFPALETGIYAILDGILISSSQDTHLGKKQSKLLEEYVRQNRLFCQKGFDQEW
                     MNQLKQNLDRLAKTENNRIASAVNKKMQKLFAGRQCNTR"
     gene            1379..2212
                     /locus_tag="U6309_002829"
     CDS             1379..2212
                     /locus_tag="U6309_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2541 family Dot/Icm T4SS effector"
                     /protein_id="HEN5506239.1"
                     /translation="MLMEFEDWFLEQISNGKKCILIGENHYSYASEDCLDCILKLQRQ
                     GKINKKIVFFDENLAALDNLEPYTQESLSKLNTVEPSLIALIKNNVEIYGLEDNFSNP
                     FKNLKSIKKVTARLKDLGLQIGNRIFNDLEHCLYWACHLYGKSLVRVQRGNEGFCNQI
                     LHKMDEDTIVIARVGAKHIPTVRKDGVAIEEGMLQRLGKDMTSACFITHFPKNKNYSE
                     QKTYQPESDMFGSYDSIECCMVQKKMIHQPDLTLWAEQDKQRKDTNDCKDTKKHRCTI
                     M"
     gene            2328..2711
                     /gene="mscL"
                     /locus_tag="U6309_002830"
     CDS             2328..2711
                     /gene="mscL"
                     /locus_tag="U6309_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEN5506240.1"
                     /translation="MSLLKEFKEFAMRGNVMDLAVAVVMGVAFNKIVTALVDGIIMPC
                     VGLLLGGVNIAGLSFIVGDAQIKWGNFLQNVIDFIIVAFAIFVLIKLINLLQRKKANE
                     PEPVTSEIQLLTEIRDLLARNSSKI"
     gene            2923..>3065
                     /locus_tag="U6309_002831"
     CDS             2923..>3065
                     /locus_tag="U6309_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2539 family Dot/Icm T4SS effector"
                     /protein_id="HEN5506241.1"
                     /translation="MKLIINKKNRAESMALDVEATTTVNELLLILYTHRSKRYEPLSF
                     SEY"
BASE COUNT         1022 a          473 c          609 g          961 t
ORIGIN      
        1 cgaataagac ttttattacc gatggattgg ctacctgtgc tgctgttgct atctacggca
       61 gtgatagcgg ttgttctgcc gcatttactc atatgtccag tgaagcttcg gacaaggatg
      121 ataaacgaaa agaaacgata ttaaatgaaa tgttggaata tgttttaaaa agcaatgcat
      181 tatccgatgt taaattaatt atttcgcctt ccaggattca agaaaaacat ttgatttcct
      241 ttattctgga atgggtaaag caaaaaaaaa ttgcttgctc catgctagaa acagggggtg
      301 attcagcagt atttaatata gataaggacg gtcgtgcatt aatgctttca acttcactgc
      361 aattaaagca ggacgcatcc aataaaacat ggcatggtca tggtattatc attggccaga
      421 gcgagggcat tccagtgatt gagaagcaga aaaaacactt tgtattcttt gcatatgaca
      481 tttcttttca aattaccaaa ctttcagacc ctcctaaaac agttggctta acaatggcag
      541 taagcgctta ggaataagca taagctggat aaaatggaat aaatttcgta ttttagtcaa
      601 aattcaaata tttcctcaat gctgggtaga tatgttaatt tcataatgga ttgtttttta
      661 tactcaattt gtaggtggtg tatgatgaga agacatttta ttattatcgt gcttggtata
      721 tttcagttcg gttgtatccc tttggcagaa catcataata agatttcacc tgctacgacg
      781 catgaagagt atcatgatgt taatttttcc aatttatatg aaaaaaatcg ctcttttcca
      841 gcattagaaa ccggcattta tgctattttg gatggaatct taatttcctc aagccaggat
      901 acgcatcttg gtaaaaaaca atcgaaactt ctggaagaat atgtgcgtca aaatagatta
      961 ttctgtcaaa aaggttttga tcaggagtgg atgaatcaat tgaagcagaa tttggacagg
     1021 ttagcaaaaa ccgaaaacaa tcggattgca tctgctgtaa ataaaaaaat gcaaaagctg
     1081 tttgctgggc ggcagtgcaa cacgaggtaa ccagctcgtt attttatcag gcagcctggt
     1141 ttaaatcaca tgtgaactca atctttcaaa ccattgatca caggattttt tgtacaggat
     1201 atcatgtcat gagagggggg gagctcaatt ttcaattcca tgccctgtag tccggcataa
     1261 aacgggaaag atatacaatc agcatatgat atttattata catattgatc gctatttggt
     1321 tcatctgtat aatgattacc ttggttgggt tatggctcta ttgtcttttt agtgagatgt
     1381 gctaatggaa ttcgaagatt ggtttctgga acaaataagc aatggtaaaa aatgcatctt
     1441 gataggggaa aaccattatt cctatgcaag cgaggactgt ctcgattgta ttttaaaatt
     1501 gcaaaggcaa ggtaagataa acaagaaaat agtatttttc gatgaaaatt tagctgcatt
     1561 agataatttg gaaccttaca ctcaggaatc tttaagcaaa ttaaatacag ttgaaccttc
     1621 tttaattgca ttaattaaaa ataatgttga aatttatggg ttagaagata atttcagtaa
     1681 tccatttaaa aaccttaaat ctattaaaaa agtgacggca cgtttaaaag atttgggttt
     1741 gcaaataggg aatcgaatat ttaatgacct tgaacactgt ttgtactggg cttgccatct
     1801 ctatggaaaa tcattggtca gagtacaacg cggtaacgaa ggattttgta atcaaatttt
     1861 gcataagatg gatgaagata ctatagtcat tgccagagta ggggccaaac atatccctac
     1921 ggtacgaaaa gatggagtag ctattgaaga ggggatgctg caacggttag gcaaagacat
     1981 gacttccgcc tgttttatta cacacttccc taaaaacaag aattattcag aacaaaaaac
     2041 gtatcagcca gagtctgata tgtttggaag ttatgattct atagaatgtt gcatggttca
     2101 aaagaagatg attcatcaac cggatttgac tctatgggct gaacaggata aacaaagaaa
     2161 agataccaat gactgcaaag atactaaaaa acaccgctgc acaataatgt aaattcaagt
     2221 gcctttctac aagctaacag gcttcagaac agtatgtaat tgaaagattg gaaaaatttt
     2281 gcgaatatac atttcatcgc ataacttaat catgataggg atgtctgatg agtttactaa
     2341 aggaatttaa agaatttgcc atgcgaggca acgtgatgga tttggctgtt gctgttgtaa
     2401 tgggtgttgc ttttaataaa attgtcactg ctttggtcga tggaattatc atgccctgcg
     2461 ttggtttgtt attgggaggc gtcaatattg caggattgag ttttattgta ggagacgctc
     2521 aaatcaagtg gggaaatttt ttacaaaacg tcattgattt tattatcgtt gcttttgcta
     2581 tttttgtact catcaaatta atcaatctgc tgcaaagaaa aaaagcaaat gaaccagaac
     2641 cagtcacttc ggaaatccaa ttacttactg aaatacgtga tctgcttgca agaaatagca
     2701 gtaaaatctg aaattggctg gcaaaaataa acctctattt atttgcagtc agataccccc
     2761 attcaaaatg gcttgttaaa cagcttatgt tttatttcgg tgagatttat acagaattct
     2821 ggtgtttcaa ttggctattt aaatctcaca aaaccatata ttgatttatt gctatttaaa
     2881 ctatactagc agacaaggct aatttttact taatgatcgc aaatgaaact tataattaat
     2941 aaaaaaaata gggcggagtc gatggcgttg gatgttgaag caacaacgac agtgaatgag
     3001 ttgcttttaa ttctttatac tcatcgcagt aaaaggtatg agcctttgtc ttttagtgag
     3061 tatca
//
LOCUS       DASLOE010000248         3032 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.249, whole genome shotgun sequence.
ACCESSION   DASLOE010000248 DASLOE010000000
VERSION     DASLOE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.249"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            52..861
                     /locus_tag="U6309_002832"
     CDS             52..861
                     /locus_tag="U6309_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEN5506242.1"
                     /translation="MITMGRSEMKIASTELRELMKAVSEGHYETVNTILDKDPELVNQ
                     YVPPTYDSPLARVLNKKHIDYKMLDILVKHHVDFDYPINHHKETPIELACKNQDLELF
                     KYLVQHSSPISEHAPHFLLVNSTNIKYLTEDKIKNTCEIIKLMGGLEAVSSKCDAEGN
                     RFEELARKSQLINRFGGIVRYDYMQLLRSVYPIVDREVDTPTIHASTEKLTDLLNKIR
                     DQHTSKEGYDQQNLKDSISLFFMTGGEVPPSRKVPESRFEEAGIDAPKNAL"
     gene            complement(1014..1625)
                     /locus_tag="U6309_002833"
     CDS             complement(1014..1625)
                     /locus_tag="U6309_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5506243.1"
                     /translation="MNKRNWTAYYNSTKQNTLPRKSLLKAIANFDKEKINLSKSAIDL
                     GCGAGIDVMELLRCGWSVIAIDSQLSAIDSLLSSAQELTCTNKLKTIVSSFEALDSLP
                     KVNLINASFSLPFLKPVYFYKFWNVILKGLKPGGRFSGSFFGFNDFWNTRTDMIFFNR
                     EMICHLFSQFKIEYFQEEEKDEMDALGYYKHWHKFFIVAKKIA"
     gene            1887..2318
                     /locus_tag="U6309_002834"
     CDS             1887..2318
                     /locus_tag="U6309_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506244.1"
                     /translation="MHPDLKDLLDYRNHKIITRYNMDYPTAAMQAEEALHELMKFIWL
                     CHKHKTDKLLYPDDKNLDFSCVIHAEMADIDKMWHTFLLFTRDYHEFCDTCLNGIFFH
                     HDPLIGENTPVSEEDYAMELTRYLTYIHDHLGSETLIKWFQ"
     gene            complement(2302..2700)
                     /locus_tag="U6309_002835"
     CDS             complement(2302..2700)
                     /locus_tag="U6309_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN5506245.1"
                     /translation="MSHPGYSVYNEKCPSHNVLEGISDKWSILVISLLSQKIYRFGEL
                     KREIGGISPKMLTQTLLKLERYGFVQRQSYPVLPMKVEYSLTRLGKELSAILVLLTRW
                     TETNMDKIINAEKTFCEQKLGCHARFIETI"
     gene            complement(2722..>3032)
                     /locus_tag="U6309_002836"
     CDS             complement(2722..>3032)
                     /locus_tag="U6309_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN5506246.1"
                     /translation="SNTEKINTLLFVEKLRGQFTEHYLPISISSENLKLDFKTSEHTK
                     KRIDIFQETIWEIRRLVFALCISEFVLHSSTASEQHLQRFKLLMNKARTHFIQFEGRY
                     "
BASE COUNT         1023 a          562 c          499 g          948 t
ORIGIN      
        1 aagattcagc cagtttgatt tttagaatac tatggtgata gtttaaaata ggtgattaca
       61 atgggtagaa gtgaaatgaa aatagcatca actgaattgc gtgaattgat gaaggcagta
      121 tctgaaggtc attatgaaac tgtcaataca atactggata aagatcctga acttgtaaat
      181 cagtacgtcc cccccactta cgattccccc ctggcaagag tattaaataa aaaacacatt
      241 gactacaaaa tgcttgatat tttggttaag caccatgttg attttgatta tcccatcaac
      301 catcacaaag aaacaccaat tgaattggct tgtaaaaatc aggatcttga attattcaaa
      361 tacttagtgc aacacagctc ccccatttca gaacatgcac cacatttcct gctggtgaat
      421 agcaccaata tcaaatacct gacagaagat aaaattaaaa atacctgtga aattattaaa
      481 ttaatgggtg gtttggaagc tgtctcttct aaatgtgatg cggaaggtaa tcgcttcgag
      541 gaactggcaa ggaaatcaca attaatcaat cgtttcgggg gtatagtaag gtacgattac
      601 atgcagttgc tgcgatcggt ttatccaatt gttgatagag aagtggatac tccaaccatt
      661 catgcctcta ctgaaaaact tacagacctg ctcaataaaa ttagggatca gcacacctca
      721 aaagaaggat acgatcaaca aaatttaaaa gattctatca gccttttctt tatgacaggc
      781 ggcgaagtac ctccttcaag aaaagttcca gagagtcgtt ttgaagaagc tggcatagac
      841 gcccctaaaa acgcgcttta aatttattta tcctatctat taagtcgtca agaagcacca
      901 aatttactat ttggcgcttc ttggcacctc gcatctctcc tttatataac tctcaactca
      961 taagacttga ttgtgccttc caagccaata catacgattt tatgattacc catttatgca
     1021 atttttttgg ctacaataaa aaatttatgc caatgcttat aataacccaa ggcatccatt
     1081 tcatcctttt cttcttcttg aaaatattca attttgaatt gggaaaacaa atggcaaatc
     1141 atttcccgat taaaaaaaat catatccgtt ctggtattcc agaagtcatt aaaaccaaaa
     1201 aacgaaccag aaaatcgacc acctggctta agccctttta aaataacgtt ccagaattta
     1261 taaaagtaaa ctggcttgag aaatggaaga ctaaagctgg cgttaatcaa atttacttta
     1321 gggagtgaat ccaaagcctc aaatgaagaa actattgttt ttagtttatt cgtgcatgtt
     1381 aattcttgag ctgaagaaag caaactgtct atagcagata attgtgaatc aatggcaatc
     1441 acagaccaac cgcatcgcaa aagttccata acatcgatac cggcaccaca tcccaaatcg
     1501 attgccgatt tagatagatt gatcttttcc ttatcaaaat ttgcaattgc tttaagcaaa
     1561 gactttctgg ggagagtgtt ttgtttcgta gaattataat aagccgtcca atttctttta
     1621 ttcatttact atatcccctc tagttaccaa aaggtaacta attgactgat tttattaaat
     1681 attttagtct cgaacctgtt cattttataa aataaaggag tggattatga aagaaaatct
     1741 cattaaaaaa attgtccaga aaaaactgct aaaaaggaat agaggatggt gtggtccagg
     1801 aacttgtatt ggatgatatt actgcaatga tggcacgtta tcaaaaaacg tgccgttaca
     1861 cttaaaccaa tcaaagcccc ataatcatgc atcctgactt aaaagactta cttgattaca
     1921 gaaatcataa aattattacc agatataata tggattaccc gactgcagcc atgcaagctg
     1981 aagaagcatt acatgaatta atgaaattta tttggctctg ccataaacat aaaacagata
     2041 aattacttta tcctgatgat aaaaatctgg atttctcttg tgtcattcat gctgaaatgg
     2101 ctgatattga caagatgtgg catacttttc ttttattcac cagagattat catgagtttt
     2161 gtgatacctg tctcaatgga attttttttc atcatgaccc tttaattggg gaaaataccc
     2221 ctgtttctga agaggattat gctatggagt tgacccgcta tttaacctat atccatgacc
     2281 atttaggttc ggaaacttta atcaaatggt ttcaataaat cgagcatgac aaccaagctt
     2341 ctgttcgcaa aatgtttttt ccgcattgat tattttatcc atattggttt cagtccaacg
     2401 agtaagtaaa accaatatgg cacttaattc cttacccaat ctggttaatg aatactcaac
     2461 cttcataggt aaaacaggat aagattgtct ttggacaaag ccatatcgct ccaatttcag
     2521 taatgtttgc gtcaacattt taggtgatat tccaccgatt tcacgtttta actctccaaa
     2581 acggtatatt ttttgggata aaaggctaat aaccaatata gaccatttat cactaatacc
     2641 ttcaagcaca ttatgcgatg ggcacttctc gttataaaca ctataaccag gatgagacat
     2701 atttgtactc cctatttcta attaataacg accttcaaac tgaataaaat gggttcgagc
     2761 tttattcatc agtaacttaa atcgttgcaa atgctgttca gatgccgttg aggaatgtaa
     2821 aacaaactct gaaatacata atgcaaaaac caagcgtcgt atttcccaaa tcgtttcttg
     2881 aaaaatatca attctttttt tagtatgttc acttgtttta aaatcgagtt tcaaattttc
     2941 ggagctgatg gatatcggga gataatgctc ggtgaattgg ccacgtaatt tctctacaaa
     3001 gagcaatgta ttaatttttt ctgtattgga tt
//
LOCUS       DASLOE010000249         3026 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.250, whole genome shotgun sequence.
ACCESSION   DASLOE010000249 DASLOE010000000
VERSION     DASLOE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.250"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(284..1138)
                     /locus_tag="U6309_002837"
     CDS             complement(284..1138)
                     /locus_tag="U6309_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpg2555 family Dot/Icm T4SS effector hydrolase"
                     /protein_id="HEN5506247.1"
                     /translation="MELTMAKELVFIDWDDTLVNRENAEDFESVTDENNTIYQMSPMF
                     IGAVEALKQLQEKYHVVIVSSRGDLNRLRQLQLQGYDLESSIMPLDNFTFEDNDNRLL
                     FTRYGETNFPDKASFILYIQKMSGNKIKVFIDDDHTEIEAAQSKGIPTIHATHKKYLQ
                     NTEQFFSGLIHQVDNMELSHTFNKVAAEIRNNSRSLSWGRQIAGFFSVHLPIAQKVEN
                     LASKLQRKSANEVSEEFDQYLKKIAYDPNKKGDIAASLTKAKTAIDKHMSASIEIKEV
                     QPFLNRMV"
     gene            1709..>3026
                     /locus_tag="U6309_002838"
     CDS             1709..>3026
                     /locus_tag="U6309_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEN5506248.1"
                     /translation="MFDRNIKEIINELQEKDLLPDTGKIIGGPRNQFDKKGEVKKGYL
                     FSVIDKEPIGKGGFASVYPVTLYRQDETGFAKKSTGHKKFVTKKFNLNKLKIDHSDLN
                     IENLIEKEFEFCRKAGHLSIKPPVYDNNKCYLTMKRMPGMDLNRIFDKLMANPHYLTD
                     NQRIKITYALLLALKNQVIDKGIIHRDIKPENIHVNVVDPVEVNIFDYGLAKNAGEED
                     NLFPGSANWAAPEMFSGLKHDNKADIFSMGRVIAFLWGESVANYLSNNPLNKSQKAAK
                     NLNLPARVKWVEPFLKQMLAERQEDRIDIDSAINEFKQLASINAPKLKLSQSSIKNHS
                     NINNLRFFQNPTKSTNNSHPTQLNIQQDIKRDLQELLENLPSEFSHYRSNIIFKHTIG
                     RFTTLQKMMNELNQIMDENESQDIPKALYDFIHQYQNKFLHASEANI"
BASE COUNT         1050 a          475 c          510 g          991 t
ORIGIN      
        1 gtagagaatc attattcaat aagtcaacag cataaaatta aatcgcaact ggatgattga
       61 aatattgatt attacagaaa tcaatgtatt tttcctttga ccattgctat tgactttaga
      121 tatcgataca tatacctaaa tctcgagatt tgtttgcagg atctcaaagt tatccaccag
      181 gattaaatcc cgaggtaagc cccgggatgt cgattgagga agctaaagtg aatgacagta
      241 gttcctgggg cataccaagg cacctagata atggagagaa ttgttaaacc attctattga
      301 gaaatggttg aacttctttg atttcaatcg aggctgacat atgtttatct attgctgttt
      361 tagctttagt taaacttgcg gctatatccc cttttttatt tggatcataa gcaattttct
      421 taaggtattg atcaaattct tcgctaacct cgttagcaga ttttctttgt aatttagatg
      481 ccaaattttc taccttctgt gctataggca ggtgaactga aaagaaacca gcaatttgcc
      541 ttccccagga aagtgaacga gaattgttac gtatttcagc tgctacttta ttaaaagtat
      601 gagaaagttc catgttatct acttgatgaa ttaatcctga aaaaaattgc tctgtatttt
      661 gcagatattt tttatgagtc gcatgtattg tgggtattcc ttttgactgt gcggcttcta
      721 tttctgtatg gtcatcatcg ataaatactt ttatcttatt accgctcatt ttttggatat
      781 acaaaatgaa acttgcttta tcaggaaagt ttgtttcacc ataccttgta aataataaac
      841 gattatcatt gtcttcaaat gtgaaattgt ctaatggcat aattgaagac tctagatcat
      901 aaccttgtaa ttggagctgt cttaatctat ttaagtctcc tcgtgaactg acgatcacaa
      961 catgatattt ttcttgaagt tgtttcagcg cttctactgc cccaataaac ataggagaca
     1021 tttgataaat agtattgttt tcgtctgtta ccgattcaaa atcttctgcg ttctctctat
     1081 tgactaatgt atcatcccaa tcaataaaaa ctagttcttt ggccatagtt aactccagat
     1141 taatcttaaa taacttaatt taacaatcac atgatcataa agattaaatt ttttacaatt
     1201 atagcccggt agagcaatag aaataactgt cattttttaa cagtgaaata ggaataatct
     1261 aagtgtaaaa agaataggat cagattaatc ctaggtggaa atgaaggttt tttaaatttt
     1321 ttcattcatt attgtaggct caaccctatg acaaaaccta caattaatca ataatgcggt
     1381 atcaatcttt tgctgatcaa gatcattatc agtaatttcg agtgctaaaa actaatcatt
     1441 tggtagtttc tttttcacaa atctgtctgc tcgtccatgt gtttggatca tcttaattgc
     1501 tccagtaatt ttcatttatt aaatcctgta gtttaagcaa tagaaacaga aacaagttgt
     1561 cggcgtgact ataggtaaaa aaataagtgc cataactgtt caatttcgac agttatttta
     1621 tagatcaaaa tggttaaaat atgtctaatt atttcgattt agacgagtta gcaaatacat
     1681 gaataactat tttgggagta attaaaatat gtttgataga aatataaaag aaataatcaa
     1741 tgaattacaa gaaaaagatt tacttccaga cactggaaaa attatcggcg gtccccgaaa
     1801 tcaatttgat aagaaaggag aggtaaaaaa agggtatctt ttttcagtta ttgacaaaga
     1861 accaataggt aaaggtggat tcgcttctgt ttatcctgtt acgctgtatc ggcaagatga
     1921 aactggattt gctaaaaaat ctactggtca taagaagttt gtaacaaaga aatttaattt
     1981 aaataaactt aaaatagacc atagcgactt aaatatagaa aatttaattg aaaaagagtt
     2041 tgagttttgt agaaaagcag ggcatttaag tataaaaccg cctgtctacg ataataataa
     2101 atgttatctg acgatgaaac gaatgccggg gatggattta aatcgtatct tcgataaatt
     2161 aatggctaat cctcattatc ttacagataa tcaacgaatt aagataacct atgctttatt
     2221 attggctttg aaaaatcagg tcattgataa aggaattatt caccgagata taaaacctga
     2281 gaatattcat gttaacgtgg ttgaccctgt tgaagtgaat attttcgatt acggattagc
     2341 caaaaatgca ggtgaagagg ataacctatt tcctggaagt gcaaattggg cagcgcctga
     2401 aatgttttct ggcttaaagc atgataataa agctgatatt ttttcaatgg gaagagtgat
     2461 tgccttccta tggggggaaa gtgttgcaaa ctatttgagt aataacccac ttaataaaag
     2521 ccaaaaagcc gcaaaaaacc ttaatttgcc tgcacgagtt aaatgggtag agccattttt
     2581 gaaacaaatg ttagcggaaa gacaagagga tagaatagac attgattccg caataaacga
     2641 attcaagcaa cttgcatcca ttaacgcgcc gaagcttaaa ttatctcagt cctcaataaa
     2701 gaaccattca aatattaata atctccgctt ttttcaaaat ccaactaaaa gcacaaacaa
     2761 ctctcatcct acacaattaa atattcagca ggatattaaa agggatttgc aggaattatt
     2821 agagaatctt cctagtgaat tttctcatta tcgcagtaat ataattttta aacacacgat
     2881 tggtcgtttc acaacactac aaaaaatgat gaatgaacta aatcagatca tggatgaaaa
     2941 tgaatctcaa gatataccca aggcattata tgattttatt catcagtatc agaataaatt
     3001 tttgcatgca agcgaagcga atatta
//
LOCUS       DASLOE010000250         2977 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.251, whole genome shotgun sequence.
ACCESSION   DASLOE010000250 DASLOE010000000
VERSION     DASLOE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.251"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            51..554
                     /locus_tag="U6309_002839"
     CDS             51..554
                     /locus_tag="U6309_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506249.1"
                     /translation="MPKKFTRKIPLISREECFALLPGYSINAMNLLKRGNHNPYYEEI
                     ESLSKIFVALITHYSEPIESIINCMEKAMYDAYKEELDKIEHEIQRLFKIRKNTPIHS
                     LFGEREIEKLTIQISELEKCKSVSVKEIIQGVIYDTLDKASQTLVDRIPGNYKLQEYE
                     SRGNPFQ"
     gene            817..1296
                     /locus_tag="U6309_002840"
     CDS             817..1296
                     /locus_tag="U6309_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEN5506250.1"
                     /translation="MTLCPCGSQNKYEQCCGIYLESKKAPETPEQLMRSRYTAYSLAK
                     IDYIKQTMQGKPLIAFNESEAKQWAQNVSWLGLKIIQSHMESPDKGFVEFIATFLDGN
                     QLKNIHEISEFQKDNSVWFYVDGEDKPLPNKQDKQKIGRNSQCPCGSGKKFKNCHDK"
     gene            1316..>2977
                     /locus_tag="U6309_002841"
     CDS             1316..>2977
                     /locus_tag="U6309_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN5506251.1"
                     /translation="MNGHLLLNVFIFLAAASIMVPLASRFRLGSVIGYLTVGMLIGPF
                     GLKLIGNAEQIMHFAEFGVVMMLFLIGLELEPIMLWKLRKLIVGLGGLQVLLTTIALS
                     SAAVMMGYNLRASVAVSMALALSSTALVLQMLQEKNLLKTAEGETSFAVLLFQDIAVI
                     PILVILPLLGNHHGINVAINPHTAASIAQLPKWLHAIVVAGIIGSIVLAGHYLSRYLF
                     LVIAKTNIREVFTAFSLSLVVGITLLMETIGVSPALGAFIAGMVLANSQYKRTVEADI
                     QPFKGLLLGLFFISVGMGINFNLLFSQMAVIIPIVLGLITIKALILFTLGKIFQLTKI
                     QALGFALGLSQGGEFAFVLFQYSSTLKVISPEISSFFTLVVALSMLATPFLMLLYHRF
                     MMPKLISKLPEREYDSIHEKNNIIIAGYGRFGQIIGRFLSGENIKVTVLEKNPEQVEL
                     LRKFGYTGYFGDANRLDLLKSAGAAHAQLLIVTVGNPDTNLEIVKLAKEEFPQLKIFA
                     RARNRRHAYELHKAGVHYFKRELFDSSLTMTKEILKFLGYKPEEIE"
BASE COUNT          941 a          547 c          577 g          912 t
ORIGIN      
        1 catatgggtg ctatacttat aatgtagacc tgatgaaaag gatgttcact atgcctaaga
       61 aatttactcg aaaaatacca cttatttcca gagaagagtg tttcgcttta ttacctgggt
      121 acagcatcaa tgcaatgaat cttctgaaac gagggaatca caacccctat tatgaagaaa
      181 ttgagtcctt atcaaaaatt tttgttgcct tgatcactca ttattcagaa ccaatagaat
      241 caatcatcaa ttgcatggaa aaagcaatgt atgatgctta taaagaggaa ttagataaaa
      301 ttgagcacga aattcaacgc ctgtttaaga taagaaaaaa tactccaatt cattctctat
      361 ttggcgaaag agaaatcgaa aaattgacta tacagatttc agagttggaa aaatgcaaat
      421 cagtgtctgt aaaggaaatt atccagggag ttatctacga taccttagat aaggcaagcc
      481 aaactctggt ggatagaata ccgggaaact ataaactgca agaatatgag agccgaggca
      541 atccttttca ataagattca gttcattgtt aaatgaaaca caaccaatca cttataacct
      601 aatttactcc gatagcactt ggtcattttt ttattaccca ttttaaaatc gctgtgtgga
      661 ttaataatga attacacttt gtgcctgttt ttataaaaaa atccaatgca acaaaacagt
      721 ttagaaataa tggaatgccg tatagaatat aatccgggat tcgttaatat aacggattgt
      781 aaatttaccc ataaaccagt ttttacaggt aactaaatga ctctttgccc ctgtggttca
      841 caaaataaat atgagcaatg ttgcgggatt tatctggaaa gcaagaaagc tcctgaaaca
      901 ccagaacaat taatgcgttc aagatatact gcgtattctc ttgcaaaaat cgattatatt
      961 aaacagacca tgcaaggcaa accactaatt gctttcaacg aatcagaagc aaagcagtgg
     1021 gctcaaaatg tctcctggct gggcttgaaa atcattcaaa gccacatgga gagtcccgat
     1081 aaaggttttg ttgaatttat agctaccttt ctcgacggaa atcagttgaa aaatattcat
     1141 gaaataagcg agtttcaaaa ggataattcc gtttggtttt atgtggatgg agaagataaa
     1201 ccgttaccaa acaaacaaga taagcaaaag ataggccgca acagtcaatg tccctgtgga
     1261 agtggtaaaa aatttaaaaa ctgtcatgat aaatgaatta aactcaaggt agcagatgaa
     1321 cggtcactta ctcctcaatg ttttcatttt ccttgctgct gcaagcatca tggttccact
     1381 ggcaagccga tttagacttg gttctgtgat tggttatctt accgtgggga tgcttatcgg
     1441 gccttttgga cttaaactaa taggcaatgc cgaacaaatt atgcatttcg cagaatttgg
     1501 agtagtcatg atgttattct tgattggttt ggaattagaa ccaatcatgc tctggaagct
     1561 gagaaaatta atagttggct taggtggctt acaagtgttg ttaacaacaa tcgctctgtc
     1621 ttcagcagct gttatgatgg gatacaattt gcgagccagt gtagcggtca gtatggcact
     1681 tgccctatct tcaactgctt tagtcttgca aatgctccag gagaaaaatc ttttgaaaac
     1741 cgcggagggt gaaacttctt ttgctgttct cttattccag gatattgcgg tcataccaat
     1801 cttggtgatt ttgcctttat tgggcaatca tcatggaatt aatgtggcta ttaaccctca
     1861 tacggctgct tctattgctc aattacccaa gtggcttcac gctattgtcg ttgccggtat
     1921 tattggtagc attgttttgg cgggacacta cttatccaga tacttgttcc tagtcattgc
     1981 aaaaaccaat atccgtgaag tatttacagc attctcctta tccctggtcg ttggaattac
     2041 cttgttaatg gaaacaattg gggtgtctcc ggctttggga gcttttatcg caggtatggt
     2101 tttagctaac tctcaatata aacggactgt agaagctgat attcaacctt tcaaaggact
     2161 gcttcttgga ttatttttca tctcagtcgg aatgggtatc aacttcaatc tactcttcag
     2221 tcaaatggct gtaatcatcc ctattgtact cggtttgatt accattaagg cattgattct
     2281 ctttacactc ggcaagatat ttcaactcac taaaatacag gcgctcggat tcgcccttgg
     2341 tttatcacaa ggtggggaat ttgcctttgt tctttttcaa tactccagca cattaaaagt
     2401 aatcagcccg gaaataagca gttttttcac tctggtagtc gctctgtcca tgttggctac
     2461 acccttttta atgcttttgt accatcgttt tatgatgccg aaattaataa gcaaattacc
     2521 agaacgtgaa tacgactcta ttcatgaaaa aaataacatt attattgctg gttacggtcg
     2581 atttggtcag ataattggtc gatttcttag tggtgaaaac atcaaggtaa ccgttttgga
     2641 aaaaaatccg gagcaagtcg aattattaag aaaatttggc tacaccggat attttggtga
     2701 tgcgaatcgt ctggacctgc taaaaagcgc aggagcagcc catgctcagt tgctcatagt
     2761 cacagtcggg aatcccgaca ccaatttgga aattgtgaaa ttagctaaag aagagtttcc
     2821 tcaattgaaa atttttgccc gagcaagaaa tagacgacac gcctatgagt tacataaagc
     2881 aggagttcat tatttcaaaa gagaattatt tgactcttcg ttaaccatga ccaaggaaat
     2941 tttaaaattt cttggttata aacctgaaga aattgag
//
LOCUS       DASLOE010000251         2912 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.252, whole genome shotgun sequence.
ACCESSION   DASLOE010000251 DASLOE010000000
VERSION     DASLOE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.252"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            125..382
                     /locus_tag="U6309_002842"
     CDS             125..382
                     /locus_tag="U6309_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506252.1"
                     /translation="MSHNNKIGIYKGIIQYLLDATNYPLQRIANLSNSPIAYLQLIYH
                     HNRLLQNRSVELNLLKLFIVFIDMEIKREQKAEKLFPADMH"
     gene            394..597
                     /locus_tag="U6309_002843"
     CDS             394..597
                     /locus_tag="U6309_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HEN5506253.1"
                     /translation="MQFSDNKMPKQILFITDLEELLGRNRLTLRRWWTSGKFPQPVKL
                     NGTTLAWHSETVNHWIQQNMRLS"
     gene            complement(621..1562)
                     /locus_tag="U6309_002844"
     CDS             complement(621..1562)
                     /locus_tag="U6309_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEN5506254.1"
                     /translation="MRIAMNNPLIKYLKPALSFQDQLEKLQAKGLIINNWPSALQSLS
                     NTNYYRLSAYCLPFKRSDTTGNITEQFQDNVTFENVIDLYEFDRKLRLLVMDGLERIE
                     ISVRTSIAYHLAHSYGPFALSNPQNFHQQFEHNTWLAQINNEIERSREYFIEHYKNKY
                     LGYPNLPIWMAIEVLSFGALSVLFKGLKNEDKRIIAEGYKLHPKTLANWLYFLTYVRN
                     ICAHHSRLWNKDLAIKPKIDAINELWLPPITPRNDRSYIILLIIRKLLTTAGNGKDWA
                     ISSEKLIQPIIEKYDWAHESMGIPRSWLDHPLWKEVQ"
     gene            complement(1652..2833)
                     /locus_tag="U6309_002845"
     CDS             complement(1652..2833)
                     /locus_tag="U6309_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN5506255.1"
                     /translation="MKFIDSYIKNLAPETTWFEKIESSGLGIRVMPSGNKSWFYRFSM
                     NGKRQKMTLGKYPAISLKQAREYLAKAQSLKEQGINPIENTKLEKLKEDNTFSKLIQS
                     WYENYAVKNRKQPRPIKYQIDSEIIPLLGDTVLDKLQTKDITIALDKIVQRGAPIHAN
                     RILSTIKQVLNYAVSRGYIQYNPATNIRSRDIGGIEKPRERVLSPEEIRIIWKFLESD
                     KSQMSESARLAIKVIILTGVRTGEIRLAQWHQFDFEQSLWTIPPEHSKGGITVKIHLS
                     ELTKKLLLQFKEQSVSPFVIPGITIDVPMSKDALPRAIKRIQNRVGIPEWTAHDLRRT
                     FATQLGESLNIDPVVIEKCLGHKMPRIMATYNKNEMLPQRKEALDSWANHIARLISSK
                     D"
BASE COUNT          863 a          504 c          526 g         1019 t
ORIGIN      
        1 ttctttaaaa cacatattga taataaaatt aatattgttt taatataagt ttcgtcaaat
       61 gattctaaat gttaatttga cgtgaattgg actttagtac acatgattta caggagtatt
      121 tttcatgtcc cataataata aaataggtat ttacaagggg attatccagt atttgctaga
      181 tgcaactaac tatccgttgc agcgaattgc taacttatct aactcaccaa ttgcatattt
      241 gcaattgatt tatcaccata accgattgct tcaaaataga agtgtagagc taaatttgct
      301 gaaattattc attgtgttta tcgatatgga aattaaacga gagcagaaag ctgagaagct
      361 ttttccagct gacatgcatt aggaggcttt aagatgcagt tttctgataa taaaatgcca
      421 aaacagattt tgtttattac tgatttagag gagcttcttg gtcgtaaccg tttgacatta
      481 aggcgttggt ggactagcgg aaaatttcca caaccagtta aactaaatgg cacaacactg
      541 gcatggcact ctgagacagt taatcattgg attcaacaaa acatgcgatt atcttagcat
      601 aatagcccat attatgtggg ctattgaact tctttccaca atggatgatc taaccagctc
      661 cttggaattc ccatgctttc atgcgcccaa tcatattttt caataatagg ctgaataagt
      721 ttctcggatg atattgccca atctttgcca ttacccgcag tggttagtaa ttttctgatg
      781 atcaacaaga taatatatga gcgatcattt cttggtgtaa taggaggtag ccaaagttca
      841 ttaatcgcat caattttggg tttaatggct aaatctttat tccaaaggcg gctatgatga
      901 gcacatatat ttcttacata agttagaaag tatagccaat tggctaaagt ttttggatgt
      961 aacttataac cctcagcaat tatccttttg tcttcatttt taagcccttt aaataagacc
     1021 gacaatgcac caaaggataa aacctctatt gccatccata ttggtaagtt aggatagccc
     1081 agatatttat ttttataatg ctcaataaag tactctctgg aacgctctat ctcgttatta
     1141 atttgtgcta accaggtatt atgttcaaat tgctgatgga aattttgagg attcgacaaa
     1201 gcaaatggtc cataactatg agcaaggtga taagcaatac tggttcttac tgaaatttca
     1261 attcgctcta atccatccat tactaacaat cttagtttac gatcaaactc atagagatct
     1321 atgacatttt caaatgtaac attatcttga aattgttcgg tgatatttcc tgttgtatct
     1381 gaacgtttaa agggtaaaca gtaggcacta agccgataat agttggtatt agaaaggctt
     1441 tgcagggctg aaggccaatt atttataata agcccttttg cttgaagttt ttccagttga
     1501 tcttggaaac tcagggctgg ctttagatat ttaatgagag gattattcat agcaatacgc
     1561 ataaaaaaac cgccaaggtt acgcaaggac taacatcctc taggcgtggc ggttgtgtta
     1621 agttaattat agagcagttc atatgatttt ttcaatcctt tgatgagatt aatcttgcaa
     1681 tatggtttgc ccaggaatcc aatgcctctt tgcgttgggg tagcatctca tttttattat
     1741 aagtagccat aatacgaggc attttgtggc caagacattt ttctatcacc acgggatcaa
     1801 tatttagtga ctcacccaat tgtgtcgcaa aggttcttct taagtcatgg gcagtccatt
     1861 caggaattcc cacccgattc tgaattcttt taattgcgcg tggaagggca tctttggaca
     1921 taggaacatc gattgttatg cctggaatta caaaagggga gacagattgt tctttaaact
     1981 gaagtagtaa tttttttgta agttcactca agtgtatttt tacagttatt cctccttttg
     2041 aatgctcggg aggaatagtc cataaagatt gctcaaaatc aaattgatgc cattgtgcca
     2101 gtcggatttc acctgttcta acaccagtaa gtataataac tttaattgct aacctggctg
     2161 attcagacat ttggctttta tcgctttcaa ggaatttcca tattattctt atttcttcgg
     2221 gcgatagtac ccgctccctg ggcttttcaa taccaccgat atcacgtgat cgtatattgg
     2281 tagctggatt atattgaata taaccacgac tcactgcata gtttagaact tgtttaatag
     2341 tgctgagaat gcgattcgca tgaattggcg ctcctcgttg tactattttg tcaagggcga
     2401 ttgtaatatc tttggtttgt aacttatcaa gtacagtatc gcctagtaga ggaattattt
     2461 ctgagtcaat ctgatatttg atagggcgtg gttgctttct atttttaacg gcataatttt
     2521 cgtaccaaga ctgtattaat tttgaaaagg tattgtcttc ttttaatttt tctagcttgg
     2581 tattttctat aggatttata ccttgctctt ttaaactttg cgctttagca agatattcac
     2641 gagcctgttt tagactaata gctggatatt ttcctaaggt cattttttga cgtttaccat
     2701 tcatactaaa acggtaaaac caagatttat taccacttgg cattactcta atacctaatc
     2761 cagaagattc tattttttca aaccaggtgg tctctggcgc gagattttta atatagctat
     2821 cgataaattt catctacaca gtccttttta ttttggtacc atttttggta ccaaaattta
     2881 tgtataggta tattatcaaa tgatactaat tg
//
LOCUS       DASLOE010000252         2901 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.253, whole genome shotgun sequence.
ACCESSION   DASLOE010000252 DASLOE010000000
VERSION     DASLOE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.253"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            97..315
                     /locus_tag="U6309_002846"
     CDS             97..315
                     /locus_tag="U6309_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506256.1"
                     /translation="MELLDAVEALVKNPLLDTVALEDWVLSKSLVESYKNSDTHKIRE
                     LITDKKNFSDMSHVIPYPDMSHVFPVTK"
     gene            324..1094
                     /locus_tag="U6309_002847"
     CDS             324..1094
                     /locus_tag="U6309_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HEN5506257.1"
                     /translation="MKVVALVGTGIKTIAHITEEAKGYISTCDKVIYLVNEPLLERYI
                     RKLAKSSESLDEIYFNSKSRESAYNNIAQYIYSAIEDVRSLCVVIYGHPCVFATPGLL
                     ALSMLDDSIKTVVCPGISTQDCLYADLRFDPASGGIQSFDATEYLLYDKVVDPSSHVV
                     IYQIGMVGNLGLPTNKINFEALNFIKKKLIELYKKEKKAVIYEAALYPGTHPKISEFD
                     LEQLDRQELTTLSTLYIPPDKIQRMPSSDALRLLNQVA"
     gene            1138..2343
                     /locus_tag="U6309_002848"
     CDS             1138..2343
                     /locus_tag="U6309_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5506258.1"
                     /translation="MSSTVSSSKVGFSFSDLPVTCKLLLALSFIEAIGGGLSYHIAYF
                     FSVSTNFNKLNIGFLGFSMGIGAILGSICGGYVTGKAQAKGIIGASFLLMGVAFVILA
                     KVSSFHLSVVFVLLMGFGINLFITCSNSSFLQISKNTNHSLTIAQSYKNALENTGGIV
                     AMVLIMLLAQNHFKLAMTLVGVTLILFSIVIFLKLEISNQDVAAKPSNKEEIESVYSS
                     LIPMLFSVFCIGLTYGIQKTVLGIHLNETIGNSLIIGFFFATDPILIALFQVKLSKRI
                     ERFNKHIVACIGCILLGVSTFAMGLSSTIQGMFCSLLVFTLGEMLFMAYSVALCHQYG
                     SSNHSGLGIGAWRSAYALGMMIGPLISGVAMYYFNAQSAWTISSLLCFMSAFLVFNSK
                     RAQRLEAVY"
     gene            complement(2326..>2901)
                     /locus_tag="U6309_002849"
     CDS             complement(2326..>2901)
                     /locus_tag="U6309_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506259.1"
                     /translation="YQHIFRVAFDKSNLERDFFIQDTAKYIFEILIGEKYGFSFTNTD
                     EFLPASWIGYVLKDIYKLKTEPSILIPKKHVEKNPVYFSLQHPIFRPYQGLSRKRATL
                     ISELENMKIVTNKYKELFSSNHEEWAGTILEEKAKKAQFVYYHGFENKKNWLSDIHHL
                     LKNDSGVHHLSNDTFCQDAPFFKGCISINSF"
BASE COUNT          901 a          440 c          543 g         1017 t
ORIGIN      
        1 gatttcttga taatgattga tttttgatag gataatgatt taaattttta taaaataaat
       61 agtgcaaaat tttgattgcc aaattttgga gggactatgg aattattaga tgcagttgag
      121 gcattggtga aaaatccact ccttgataca gtggctttag aggattgggt tttaagtaaa
      181 agcttggttg aatcatataa aaactcagat acacataaga tcagggagtt aatcactgat
      241 aaaaagaatt tttcggatat gagccatgtg atcccgtatc cggatatgag ccatgttttt
      301 ccagtgacca aataaggatt taagtgaaag ttgtagcgtt agttggtact ggaattaaaa
      361 caatagctca tattacagaa gaagcaaaag gatatatttc tacttgtgat aaggtaatct
      421 acttagttaa tgaacccttg ctagagcgct atattcgtaa attagcgaaa tcatcagagt
      481 cactggatga aatatacttt aactctaaat caagagaaag tgcttataat aatattgcac
      541 aatatattta tagcgcgata gaagatgtac gatcattatg tgttgttata tatggtcatc
      601 catgtgtgtt tgctactcct ggcttgctcg ctttgtctat gcttgatgat agcattaaaa
      661 cagtggtgtg ccctggtatt tcaactcaag attgtttata tgccgatctt aggtttgatc
      721 ccgcatctgg aggtatccaa tcctttgatg ctacggaata tcttctatat gataaagtag
      781 tagatccttc ttctcatgta gtaatttacc agattggtat ggttggaaat ctgggtttac
      841 caacaaataa aatcaatttt gaggctctta attttattaa aaaaaaatta atcgaactct
      901 ataaaaaaga aaaaaaagcg gttatttacg aagccgctct ttatcctggt actcatccaa
      961 agatatctga atttgattta gagcaacttg ataggcaaga gttaacaaca ctgtctactt
     1021 tgtatattcc tcccgataaa atacaacgaa tgccgagttc tgatgcatta aggttattaa
     1081 atcaagtggc atagtttagt ggatgtatct aatttttatt taatgtgagg gattacaatg
     1141 agctcaacag tcagctcttc taaagtaggg ttctctttta gtgatttacc agtcacttgt
     1201 aaattattat tggctctttc ttttatagag gctattggag gcggcctatc ttaccatatt
     1261 gcgtattttt ttagtgtaag caccaatttt aataaattaa atataggctt cttaggcttt
     1321 tcaatgggga ttggagctat tttgggcagt atatgtggtg gatacgttac gggaaaagca
     1381 caagcaaagg gtataattgg ggcaagtttc ttactcatgg gcgtcgcatt tgttattcta
     1441 gcaaaagtga gttcttttca tttatctgtt gtctttgtat tgttaatggg atttgggatc
     1501 aacttgttca tcacttgcag taactcatct ttcttgcaga tttcaaaaaa taccaatcac
     1561 agtttaacga ttgcccaatc ctataaaaat gcactggaaa atactggtgg tattgtggca
     1621 atggttttga taatgctttt agcgcaaaat cattttaaat tagcaatgac tttggtcggc
     1681 gttactttga tattgttttc aatagttatt tttctaaaat tagaaatcag taatcaggat
     1741 gtggccgcaa aacccagtaa taaagaagaa attgagtcag tctatagctc gcttattcca
     1801 atgttatttt cagtattttg catcggttta acttatggga tacaaaaaac agtactaggc
     1861 attcacttaa acgaaacaat aggaaattct ttaatcattg gatttttttt cgcaactgat
     1921 cccattttaa tcgcattatt tcaagtaaaa ttatcaaaaa ggattgagcg attcaataaa
     1981 catatagttg cttgtatcgg ctgtatttta ttaggggtgt caacgtttgc tatgggcctg
     2041 tcttctacaa tacaaggaat gttttgttcg cttttagtat ttactcttgg tgaaatgttg
     2101 tttatggcgt actccgttgc attatgtcat caatatggaa gctctaacca cagtggccta
     2161 ggtatagggg cgtggcgcag cgcatatgca ttaggcatga tgattggccc tctcatatca
     2221 ggagtagcaa tgtattattt caatgcccaa agtgcttgga caatatcttc tttgctttgt
     2281 ttcatgagtg ccttcttagt atttaattct aaaagagcac agcgattaga agctgtttat
     2341 tgagatgcat cctttaaaaa aaggcgcatc ttgacaaaaa gtgtcattgc ttaaatgatg
     2401 cactccagaa tcatttttta ataaatgatg aatgtccgaa agccaatttt ttttattctc
     2461 aaacccatgg taataaacaa attgagcttt tttggctttt tcttcaagaa ttgtaccagc
     2521 ccattcttca tgattagagg aaaatagttc tttatattta tttgtaacga ttttcatatt
     2581 ttcaagctca gaaattagag tcgctctttt tctggacaac ccttgatatg gtcgaaaaat
     2641 aggatgttga agcgaaaaat atactggatt tttttcaaca tgttttttag gtattaatat
     2701 agacggttct gtttttagct tataaatgtc ttttaaaacg tagcctatcc atgatgcagg
     2761 caagaactca tcggtattgg taaacgaaaa cccatatttt tcacctatga gaatttcaaa
     2821 gatatattta gcggtatctt gaataaaaaa atctcgttct aaatttgact tatcaaaagc
     2881 aaccctaaaa atatgttgat a
//
LOCUS       DASLOE010000253         2893 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.254, whole genome shotgun sequence.
ACCESSION   DASLOE010000253 DASLOE010000000
VERSION     DASLOE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.254"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..2141
                     /locus_tag="U6309_002850"
     CDS             <1..2141
                     /locus_tag="U6309_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEN5506260.1"
                     /translation="AITINNGNTAGSQLIESQIRGYFRQTLLQWSGFSLAAVTALLAF
                     TQYRLSNDKVALIIGLAILFSGSIEALHTLVIDGLALQAFEKNNLDALIWVFSNSVSG
                     LILIIGLSLLVHANNNKPVRLLTFILLTTFLVLAAIAVIYYAAVIIQIPDMWFYDSYV
                     SRPFELISVFIYLMLMIIVYPKTYASYPTILSDSVFYMSLTQVVLAIYIMLLSTTPYD
                     SAFNIAYFLKVVVYFIPCTCFVINYVFSYSAVLEGQKNLKIKQEELKYMASHDPLTNL
                     FNRREFEDLLDKSIATAQRNNSSLALLLIDLDNFKAINDTFGHFHGDELLKQFANRLV
                     LLVRKGDLLSRVGGDEFTLISLDIESPSSARHLAERILNELNSPYTVNGKLITVTVSI
                     GISIFPDDGNTPEDLLRKADLAMYKAKNSGKNTYQFYTDQLGYSQHRESELESHLRKA
                     LQNDEFELYYQPKFNLINMEIIGAEILLRWNNKALGNVSPNEFIPVAENTGLITELSQ
                     WVLEKSCAQILHWTNQYNKMLSFSINISPVQLTNPQFPKILERILQEYQYPAEYLELE
                     ITENSLMGDQDEISAMLNVISGLGIKLSLDDFGKEYSSLNRLKTLPIDSLKIDKTFIS
                     DIHNEHEKVVIIDIIIKLANELGMSIVAEGIETIPQLKYLVSRKCHFGQGFLLSKPLT
                     ANEFTSIAYTKLGKTKSNAKVISLINNNRF"
     gene            complement(2262..2747)
                     /locus_tag="U6309_002851"
     CDS             complement(2262..2747)
                     /locus_tag="U6309_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEN5506261.1"
                     /translation="MPSFDIVSKMNEVDLRNAVDNAVREVSTRFDFRGVEATIELKDL
                     TVTLRSESDFQVRQLEDLFRNHCSKRNLSTSGVDIEDEPVHSGKFYTLTMTFKQGIDQ
                     PTAKEIVKYIKETKAKVQTSIQGDKVRVTGKKRDDLQETIALLKKSNIELPLQYENFR
                     D"
BASE COUNT          923 a          528 c          467 g          975 t
ORIGIN      
        1 tggcaattac aataaataat ggtaatacgg caggctctca actcattgag tctcaaatta
       61 gaggttactt cagacaaact ctcttacaat ggtcaggatt ttctctcgct gcagtgacgg
      121 cattattagc atttactcaa tatcgattga gtaatgataa agttgcttta attatcgggc
      181 ttgccattct attctctggc tccattgaag cgcttcatac cctcgttatt gatggcctgg
      241 ctttgcaagc ttttgaaaaa aataatctcg atgctttaat ttgggttttt tcaaacagtg
      301 ttagtggctt aattctaatt atcggactat ctctactggt tcacgcaaac aataataaac
      361 cagtaagatt actgactttt attctactga ctactttttt agtattggct gcaatcgcag
      421 tcatttatta tgccgctgta attatacaaa ttcctgatat gtggttttat gattcttatg
      481 tttcaagacc ttttgaactc atatctgtct ttatctatct tatgctgatg attattgtgt
      541 acccaaaaac ctatgcatct tacccaacta ttttatctga ttctgtattc tatatgtcac
      601 tcacccaagt cgtgttggct atctacatca tgttattatc aacaactccg tatgacagtg
      661 cctttaatat agcatatttt cttaaagtgg ttgtttattt tataccttgc acctgttttg
      721 taattaatta cgttttttct tactcagcgg tactggaagg gcaaaaaaac ttaaaaataa
      781 aacaggaaga attaaaatat atggcctctc atgatcctct gacaaacctg tttaatcgca
      841 gggaatttga agacctgctt gataaatcca ttgctactgc ccaaagaaat aactcttccc
      901 tggctttact gcttattgat ttagataatt tcaaggcaat aaatgacaca tttggtcatt
      961 ttcatggcga tgaactttta aagcaatttg ccaatcgcct ggttttatta gttcgaaaag
     1021 gtgatctgtt atccagagta ggtggagatg aatttacatt gatttctcta gatatagaat
     1081 ccccttctag tgcccgccat ttggcagaac gcattttaaa tgaattaaac agtccctata
     1141 ctgttaatgg caaattaatt acagtaaccg tcagcattgg catatctatt ttccctgatg
     1201 atggcaacac tcctgaagat ttgttaagaa aggccgactt ggccatgtat aaagcaaaaa
     1261 actcagggaa aaatacttat caattttata ctgatcaatt aggatattca cagcacaggg
     1321 aatccgagtt ggaatctcat cttcgaaaag cattgcaaaa tgatgaattt gaactttatt
     1381 accagcctaa atttaacctg attaatatgg agattattgg cgcagaaatt cttttacgct
     1441 ggaataataa ggccctggga aatgtatctc ctaatgaatt tatacctgta gctgaaaaca
     1501 cgggattaat aacagaattg agtcaatggg tattagaaaa atcctgtgca caaatattgc
     1561 attggactaa tcaatacaac aagatgcttt ccttttccat taacatttct ccagtacaat
     1621 tgacaaaccc tcaatttcct aaaattcttg aaagaatatt acaggaatat caatatcctg
     1681 cagaatatct tgaacttgaa attactgaaa actctttaat gggggatcag gatgaaatct
     1741 ctgcgatgct taatgtaatt tccggattgg gaataaagct gtcattggat gattttggca
     1801 aggaatactc ctctcttaat agattaaaaa ctcttcctat tgacagccta aaaattgata
     1861 aaacatttat ctctgatata cacaacgaac atgaaaaagt agtcattata gacattatta
     1921 ttaaattggc taatgagtta ggaatgagta ttgttgccga gggtattgaa actattcctc
     1981 aattaaaata tcttgtgtca agaaaatgtc attttggtca aggtttcctc ctgagtaagc
     2041 ccttaactgc aaatgagttc acttcaattg catataccaa gttgggaaaa accaaatcta
     2101 acgcaaaagt aatttcatta attaacaata acagatttta attagaacca tatactggag
     2161 ctttcaatgt gaatgtcgta tagatgctat gcatttcgtg atatcattca tttaaggata
     2221 taaaaggctt ttctttgcct tttatatcga attcatatta attaatcacg aaaattttca
     2281 tactgcaaag gtaattcaat atttgatttt tttaataaag caatggtttc ttgaagatca
     2341 tctcgttttt tcccagtcac cctcacttta tccccctgaa tagaagtttg gaccttggcc
     2401 ttggtttctt tgatgtactt cactatctct ttagctgtag gttggtcaat gccttgttta
     2461 aaagtcatgg taagagtata gaattttcca ctatgcacag gttcatcttc tatatccact
     2521 cctgatgtgc tgaggttgcg ttttgagcaa tgattccgga ataaatcctc aagctgcctc
     2581 acttgaaaat cagattcaga acgcaaagta actgtcaggt ctttaagctc aatagtagcc
     2641 tcaactcctc gaaaatcaaa tcgggtactt acttcccgca ctgcattatc tactgcattg
     2701 cgcaaatcca cttcattcat tttagaaaca atatcaaaag aaggcataac caatccattt
     2761 aaattaaaat tgtaatttat caaaaaacag ggataggtga aagaaacaac acctctattt
     2821 cctttcttgc aaaaatcaaa gttgtttaaa aataaatcaa ttgattaacc gttgcataat
     2881 tgcacaaaga atg
//
LOCUS       DASLOE010000254         2854 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.255, whole genome shotgun sequence.
ACCESSION   DASLOE010000254 DASLOE010000000
VERSION     DASLOE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.255"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..893)
                     /locus_tag="U6309_002852"
     CDS             complement(<1..893)
                     /locus_tag="U6309_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN5506262.1"
                     /translation="MPKKDCILNLPGYSIKKVSGENPVYIEVSYRNVVRCIYCGNKKL
                     RKKDSFIRRIRHESIGLRRSYLCIKAHKYYCSVCSRYFNQRFPGIGKYQRASESLRKQ
                     VFHYHSKGVSQKDLARDLKLGKSTVERWYHYGYELRYKKIATRSCPRVLGLDEHSFNR
                     KVGYATTFCDLAKHKIFDVVEGRSEKDLEGYLNTLEGKEKVQVVCIDLSSSYRKLIRR
                     YFPNAMIVADRFHVIRLLNQFCLQTYQQIDPEMKYQRGLLAALRTNPNNLSVKRLNKR
                     ERYLQQQPVIAALYNFKQRLHR"
     gene            complement(975..1709)
                     /locus_tag="U6309_002853"
                     /pseudo
     CDS             complement(975..1709)
                     /locus_tag="U6309_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1853..2578
                     /locus_tag="U6309_002854"
     CDS             1853..2578
                     /locus_tag="U6309_002854"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506263.1"
                     /translation="MSSNQLVLLNSLIAQQNFSSLQAKVQTLTAEGSTRLYGTSFDTL
                     KSLAMSAHANNVLLFTDGEDSARDSQEMRQLSELVSGLLLEAKARNKFFVIFCGTDGD
                     ALTKDIAKTFDSPIIHTQSADFMSAIKDPEKLKSYGAERGLFTVKYSVHLETGPKTET
                     NTHLIADLSGQIVALDPVEINPNSTLSITIAQGDETLVDSKKSFTPPVVQQGTNPNGF
                     LNRSSAHSSTYQDPLNTSQLNMA"
BASE COUNT          865 a          608 c          484 g          897 t
ORIGIN      
        1 cgatgtaatc gctgtttaaa gttatagagt gcagcaataa caggctgttg ttgcaaatag
       61 cgctctcgtt tatttaatcg ttttacactg aggttattgg gattggttct cagtgctgcc
      121 aatagacctc gctgatattt catctcaggg tcaatctgct gataagtctg taaacagaat
      181 tggttcaata aacgaatcac atggaagcga tcggcaacaa tcatcgcatt ggggaagtaa
      241 cgcctgatta acttgcggta gctggaactt aagtcaatac agactacctg taccttttct
      301 tttccttcta atgtgtttag atagccttct aaatcctttt cagaacgtcc ctcaaccaca
      361 tcaaatattt tatgtttggc caagtcacaa aaagttgtag cataacccac tttccgatta
      421 aatgaatgtt catccagtcc taatactcgg ggacaggaac gtgtcgctat cttcttatag
      481 cgaagctcat aaccataatg ataccagcgc tctacagttg atttacccag ctttaaatcc
      541 cgggctaagt ccttttgact cactcctttg ctgtgatagt gaaatacctg tttacgcaaa
      601 ctctcgctgg ctctttgata cttgcctatc ccaggaaaac gctgattaaa ataacgacta
      661 caaacactac aataatattt atgagcctta atacataaat aactacgccg caggccaatc
      721 gactcatgac gtattcgtct tatgaaactg tcctttttac gtaatttctt attaccgcaa
      781 tagatacagc gaaccacatt gcgataactt acttcaatat atactggatt ctcaccgctc
      841 acctttttaa tagaatatcc tggtaaattt aggatacaat ctttcttggg cattttaatc
      901 ttccttttga ccgtcaaatc aaagagttag tctatactaa cttgattaaa atgccccctt
      961 tgttggggta gagccagata ttcatgatca gcaaactcag aaccctgatc aaaagtgatt
     1021 gttttaaaca ttttttgtgg taatttttca aatttcttat ttattttttc catcactcct
     1081 cgtgaatgtt tgctcgtatt ttttatgaga taaaccattc tggttttacg ctccacaagt
     1141 gtcgtaatca cttcgctttt attcccttta aattcaattg tatcgccttc ccagtgtcca
     1201 aaacgttccc ttgaatcgat ttcttcaggt ctttgagtaa tcagataagg agtgccataa
     1261 cgacatgttc tatgctttcg cctaaagagc ttttgtcttc taggtttttt aaaggctagt
     1321 gaatgataaa gcgccttatc attagattga taaacataac gataaatagt ttcaggacaa
     1381 acataaaaac tcaatttttg atgtttcatc cggccagata tctgttcggg actccatcct
     1441 ttattgacta atgaccgaac aacataatca cgtaaaattc cattattttt tagtttatta
     1501 gggcgattat tttgagctct agctaaagcc ttctgatggg ctataaccgg taaataaact
     1561 cccgattcat tattcctatt tagttctctg tatattgttg aacgatgcct ctttaattta
     1621 tgcgtgatct cgcgtacaga taatcccata tccagaaaaa caaataaacg gcgtcgatct
     1681 gttacagata attgagtata ctgtcccatg tggcagtctc ctttgctatt tcaagttccc
     1741 caagaacatg atacagcttt ttggactgtc gcacttcaga ttagaattcc ccaaaaaatt
     1801 aaaattttcc atgtagacgc aatgtagaca tgttttcgta aaggctttat ggatgagcag
     1861 caatcaactt gttctattaa atagtctaat tgcccaacaa aatttctcat cactgcaagc
     1921 aaaagttcaa acgcttacgg ccgaggggtc aactcggtta tatggaacaa gttttgatac
     1981 actaaaatct ttagctatgt cagcccatgc caacaatgtg ctgctcttta ctgatggaga
     2041 agacagcgcc cgtgatagtc aagagatgag acagctttca gaattggtta gcggcttatt
     2101 attagaagcg aaagcaagaa acaaattttt cgttatcttt tgtggcaccg atggagatgc
     2161 actaaccaaa gatattgcta aaacttttga ctcaccaatc attcatactc aatcagcaga
     2221 tttcatgagt gccattaaag atcctgaaaa gttaaagagt tatggggctg agcgtggtct
     2281 atttacagtg aagtactcag tccatcttga aacagggcca aaaacagaga ccaacaccca
     2341 tctcatagcc gatctatctg gtcaaattgt ggcattagac ccagtagaga taaatccaaa
     2401 ctcaaccctc agtattacca tagcccaggg cgacgaaaca cttgttgaca gcaaaaaaag
     2461 ttttactcct cctgtcgttc agcaaggaac aaatccaaat ggctttttga accgttcgtc
     2521 tgctcattcc agtacatacc aagatccgct gaataccagt caattaaata tggcataaag
     2581 ccgtacaatg aaaaccttga ttccgtttca ctgcatcaag gctacaccgg ttaatagcct
     2641 gtagccttgg tgtagccaaa gtcgtcacca ggattatcct taccccacca atagtaaaaa
     2701 taataaacac agtgattttc ttttgtataa aatctgttat aatgtttctg ttgccataat
     2761 taattgactt aaaatgagta attacattta ccacaacctc aatctaggcg aaatcaagcc
     2821 tactcgatat ccaagtcata agattattcc agat
//
LOCUS       DASLOE010000255         2826 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.256, whole genome shotgun sequence.
ACCESSION   DASLOE010000255 DASLOE010000000
VERSION     DASLOE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.256"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            24..1373
                     /gene="fliI"
                     /locus_tag="U6309_002855"
     CDS             24..1373
                     /gene="fliI"
                     /locus_tag="U6309_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEN5506264.1"
                     /translation="MAIYQTQLHKILSGTRNKHHSGLLHCGRISRAIGLTIEAKGFNQ
                     PVGARCLVKINDEHSVQAEVVGFSNESVYLMSIGDIQGIAPGMKIIPTGRIAQVSVGH
                     QLLGRVLNGSGMFIDDGPPSEMVETYPLIGQVINPLKRAPIEVPLDVGIRAINGLLTV
                     GRGQRIGLFAGSGVGKSVLLGMMTRFTEADVVVVGLIGERGREVKEFIECNLGLEGLR
                     RAVVVAAPADESPLMRLHGAKVATSIAEYFRDQGKHVLLLIDSLTRFAQAQREIALSI
                     GEAPATKGYPPSVFAKLPKLVERAGNGTSGGGSITAFYTVLTEGDDLQDPIADAARAI
                     LDGHVVLSRALAEQGQYPAIDLEASISRVMQGIVSEQHMKDMLLFKKYLSVYEKNKDL
                     ILLGAYAKGSDPLVDKAILAVNKLKDYLAQGMDEQVSYDESLLSLSKLISTLVGNDQ"
     gene            1370..1825
                     /gene="fliJ"
                     /locus_tag="U6309_002856"
     CDS             1370..1825
                     /gene="fliJ"
                     /locus_tag="U6309_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEN5506265.1"
                     /translation="MSDRLDRLIQLLKIKQEATQQAYIELVKAREQFNQNKARHEQLV
                     GYRQDYLQQLEVLGQQGSYVGPLRNRINFINHLDTALVQLNSYLSQLAKNRMKADLNY
                     KQAKTSEEGISKLIERVKRAELKQLQRIEQKETDEYAQKQWYSSLKHDR"
     gene            1815..>2826
                     /locus_tag="U6309_002857"
     CDS             1815..>2826
                     /locus_tag="U6309_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506266.1"
                     /translation="MTDNNLLANKFFKKIWMVLAVLIIVAAVFSSVFRALTPWAKQYK
                     GEVEQHLTSLLGQPVTVQTMETGWYWFEPVVKLKHIRIHDGHNKSIYLDRLIVGVNLF
                     KSLWNWRIQPGVLYIDDMHLVFREKDGHWRIEGISRNAMKDTDITPEKTREILDWLAK
                     QEKLIVRHVSAHLHFNDGSLIPVNELNLSIVNKGGNYKIKGEARLEQTNATVFQLLGN
                     VQFDPSQIENTNGRFYFSAINVLPAQWQNLLPKSSMQLEGGKGNVSLWVDLEKGNISS
                     IQAELRLKRLAWKLLNKKNSKLIQSFYANMGWKLDKQGWQFQADRMKLRVAGVTWPEN
                     QLS"
BASE COUNT          913 a          444 c          661 g          808 t
ORIGIN      
        1 atgggcgttt ctcaggaata gtgatggcga tttaccaaac acaactccat aaaattttgt
       61 caggaacaag gaataaacat cattccggac tgttacattg tggtagaatc agcagagcaa
      121 taggtttaac catagaagca aagggattta atcagcctgt aggagcacgt tgcctggtta
      181 aaatcaatga tgaacattct gttcaggcag aagttgttgg ttttagtaat gaaagtgttt
      241 atttaatgtc tattggagat atacaaggta tagcccctgg aatgaaaatt attcctaccg
      301 gacgcattgc tcaagtaagt gttggccatc aattgttagg aagagtatta aatggttccg
      361 gaatgtttat agacgatgga cctcctagtg aaatggttga gacttatcca ttgatagggc
      421 aagtgatcaa tcctttaaaa agagccccta ttgaagtccc attggatgtt gggattcgcg
      481 ctattaatgg cttattaact gttggccgtg gacaacggat aggtttgttc gctggaagtg
      541 gagttggcaa atcagttttg cttgggatga tgacgcgttt tactgaagct gatgttgttg
      601 tggttggatt aattggagaa cgtgggcgcg aggtaaaaga atttattgag tgcaatttgg
      661 gtctggaagg tttgaggcga gcggttgtag ttgccgctcc agcggatgaa agccctttga
      721 tgcgtttgca cggcgcgaag gttgcaacaa gcattgctga gtattttcgg gatcaaggta
      781 aacatgttct cttgctgatt gattctttaa cccgttttgc tcaggcacaa agagaaatcg
      841 cgttatcgat tggtgaggct cctgccacaa aaggatatcc gccatcggta tttgccaaat
      901 tgcctaaatt ggttgaaaga gctggaaatg ggacttcagg gggtggttca attacggcat
      961 tttatactgt cttgactgag ggagatgatt tgcaagatcc catagcagat gcggcacgag
     1021 caattttgga tggtcatgtt gtattaagca gagcgcttgc agagcaaggg cagtatcctg
     1081 caatagatct tgaagcttct attagccgag ttatgcaggg cattgtctct gaacaacata
     1141 tgaaagatat gttattgttt aaaaaatact tgtctgttta tgaaaaaaac aaggatttaa
     1201 ttttattagg agcctatgca aaaggaagcg atcccctggt ggataaagcg atattagcag
     1261 tgaataaact caaagattac ctagcgcaag gaatggatga acaagtaagc tacgatgaga
     1321 gtttattatc gttatccaaa ctgatttcaa ctttggtagg gaatgaccaa tgagtgacag
     1381 attagatcgt ttgattcagt tattaaaaat taaacaggaa gcgactcaac aggcctatat
     1441 agagttggtg aaagcaagag agcaatttaa tcaaaataaa gcaaggcatg aacaattagt
     1501 tggttacaga caagattatt tacagcaact ggaagtattg gggcaacaag gaagttacgt
     1561 agggccttta cgcaaccgaa ttaattttat taatcatttg gatactgctt tggtacaatt
     1621 aaactcctac ttgagtcaac ttgcaaaaaa caggatgaag gcggatttaa attataagca
     1681 agcaaaaact tcagaagaag gaataagtaa gttaattgaa cgcgtaaaaa gagctgaact
     1741 gaagcaatta cagcgtattg agcaaaagga aactgatgaa tatgcacaaa agcaatggta
     1801 tagtagttta aaacatgacc gataataacc ttttggcaaa taaatttttt aaaaaaattt
     1861 ggatggtatt agccgtcctt ataattgttg cagctgtctt ttccagtgta tttcgcgctc
     1921 ttactccctg ggctaaacaa tataaagggg aggtagaaca gcatttgact tcacttttgg
     1981 gtcaaccggt tactgttcaa actatggaaa cagggtggta ttggtttgaa ccagtagtca
     2041 aattaaaaca tataagaatt catgatggtc ataataagtc aatttatctt gataggttaa
     2101 ttgtcggtgt taatttattt aaatccctat ggaattggcg cattcaacct ggcgtacttt
     2161 atatcgatga tatgcatttg gtttttcgtg aaaaagacgg gcattggcgt attgagggaa
     2221 tttcacgtaa tgcaatgaaa gacactgata ttacacctga aaaaaccagg gaaatattag
     2281 attggcttgc taagcaggaa aaattaattg tacgacatgt ttccgctcac ttacatttta
     2341 acgacgggtc attaatccct gttaacgaat taaacctgtc aatagttaat aaggggggaa
     2401 attataagat aaagggggaa gcaagactcg aacaaacgaa tgcaaccgtt ttccaactgc
     2461 tagggaatgt acaattcgat ccttctcaaa ttgagaacac caatggacgc ttctattttt
     2521 cagctattaa tgttttaccc gcacaatggc aaaatttatt gccaaaatcc tcaatgcaac
     2581 tggagggagg taaaggaaac gtttcattat gggttgattt ggagaaaggg aatatttcat
     2641 caattcaggc agaattgaga cttaagcgtc tggcatggaa attattaaat aaaaaaaata
     2701 gtaagttgat ccaatcattt tatgccaaca tgggctggaa actggataaa caagggtggc
     2761 agttccaggc agaccgtatg aaactaagag tagcaggggt cacttggcca gagaaccaat
     2821 tgtcaa
//
LOCUS       DASLOE010000256         2787 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.257, whole genome shotgun sequence.
ACCESSION   DASLOE010000256 DASLOE010000000
VERSION     DASLOE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.257"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            29..1252
                     /locus_tag="U6309_002858"
     CDS             29..1252
                     /locus_tag="U6309_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2223 family Dot/Icm T4SS effector"
                     /protein_id="HEN5506267.1"
                     /translation="MVDEKNYFLKIEFARQRVIDLPAGEFKYYLYILLESIKSQNIPS
                     RHEFLAVLLKLLDTLHKLKELKTSNFLDNQCPSSQLLKELMLHLCELQQVAKTHHFTA
                     QVMVILLHIGGIISAVALSVVGGFFGGILGFVNGLCEYKPVIGFLVGTFTGTVLGGLF
                     GFRAPKKLFKNEIQRQIKFGLDGLGESLDNINQEYSSSMFGPNKIKPFSFYLDEVGGE
                     IKQLFNDDCEYEEFLNNKVDYEINSFLASFIGESLLHGCVGHHAYIKINIKNREYLIE
                     FTPQPTDTKEAPAQSETRTVSGKKIVEMLAYHKKLQETNPPTASFILTKMKPGDNDCF
                     SYVNKILIGTNQQGATIKRFANMGLIGSLLGMGIEALSPFKPDFFQTTSSVRTHKEKM
                     PDRSNDLSMNSLLPQ"
     gene            1509..2636
                     /locus_tag="U6309_002859"
     CDS             1509..2636
                     /locus_tag="U6309_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 repeat protein"
                     /protein_id="HEN5506268.1"
                     /translation="MDMKKYILSLSLLTVINSSAIATTQEGEEAYNKGYYEKAFILLS
                     PEADKGDAKAQYLLGKMYYNAQGVTYNPEKTEQLLLASANQGNVDAQVLLAGFYWYLN
                     TPEGYKKAFEWYQKAADQNNADGQYGLGYMYDTGTGVPQNSDTAMVWYKKAAEQGNSN
                     AALAIGYNYDTGTGVKKDKTQALNWYAKAADLGNASAQYNLGLMYEQGDGVPKDYQKA
                     AEYFEKAANQGHAKSQLELGYLYDSGKLGKSDLQKAAFWYQKSADLGNANAQFNLADM
                     YFYGDGVGKSLEQSVYWMQKAAEQGYGKAQNQLGIYYRDGIGVAADPVKAYAWFTAAK
                     NNGFEKAASNASDLEKSMNPEDLSKARTLGQQYTDNYKAKK"
BASE COUNT          919 a          461 c          587 g          820 t
ORIGIN      
        1 ttatgtttcc ccgtaggttt gttttttcat ggtggatgag aaaaattatt tcttgaagat
       61 agagtttgcc agacagcgtg ttatcgactt gcctgcagga gagtttaaat attatctata
      121 cattctgtta gaatcgataa aatcccaaaa tatcccatct aggcacgaat ttttagcagt
      181 actgctaaaa ttgctggata ccttgcataa attaaaagaa ttaaaaactt ccaatttttt
      241 agataatcaa tgcccatcaa gccaattact caaagaatta atgcttcacc tctgtgaatt
      301 gcaacaagtg gccaagactc atcatttcac tgctcaagtg atggttattt tactgcatat
      361 tggcggtatt atttcggctg tagctttaag cgtagtcggc ggattttttg gaggaatact
      421 gggttttgtt aacggtttat gtgagtacaa accggttata ggttttcttg ttggaacatt
      481 taccggtaca gttcttggtg ggctttttgg tttcagggca ccgaaaaagc tatttaagaa
      541 tgagatacag cggcaaataa aatttggtct tgatggctta ggtgaaagct tggacaacat
      601 caatcaagaa tattcctcat caatgtttgg tccaaataaa attaaaccat tttcatttta
      661 tcttgacgaa gtggggggcg aaattaagca acttttcaat gatgattgtg agtatgagga
      721 atttttaaat aataaggttg attatgaaat taactctttt ctggccagtt ttattggaga
      781 atccttgctt catgggtgtg taggtcacca tgcgtatata aagattaata ttaaaaatcg
      841 tgagtattta atagaattta cccctcagcc aaccgatacc aaagaagctc cagctcaaag
      901 cgaaacaaga acagtaagtg gcaaaaagat tgtagaaatg ctggcctatc ataaaaaact
      961 tcaggaaacg aatccaccga cagcgtcttt catattaact aaaatgaaac ccggtgataa
     1021 tgactgtttc agttatgtca ataaaatact cattggaaca aatcaacaag gcgctacaat
     1081 caagcgattt gccaatatgg ggctcattgg ttctttatta ggaatgggga tagaggcttt
     1141 aagtcctttt aaaccagatt tttttcaaac tacaagttca gtgagaactc ataaggaaaa
     1201 aatgcctgat cgttcgaatg atttgtcaat gaatagcctg ttacctcaat aaccagccct
     1261 tcaatatcgg atcgcaaaga taatctgtct ctttcatgtt attgctactt tgcgcgcatt
     1321 tactgcatat aaactccgcc cctcaaaacg tttgtacctt tccctaaaac aaaataatca
     1381 cgttatgcaa gagttataat atcttgatga aagatataaa aaaaaacaaa atgaaaatgg
     1441 attttataca ttgttgaatt tatgtcatat actttaaagc gatagctctt aaaaataagg
     1501 tatatatcat ggacatgaaa aaatatattt tatctctttc tctactgacg gtgattaact
     1561 cttcagctat cgctacgact caggaaggtg aggaggccta taacaagggg tattacgaaa
     1621 aagcatttat tttgttgtct cctgaagcgg ataaaggaga tgccaaggct caatatttgc
     1681 tgggtaagat gtattataat gcccaaggtg taacttataa tccggaaaaa acagagcaat
     1741 tattacttgc ctcagcgaat caaggcaatg tcgatgcgca ggtattgtta gctggtttct
     1801 attggtacct taatacacct gagggctata aaaaggcgtt tgaatggtat cagaaagcag
     1861 cggatcaaaa taatgcagat ggtcaatatg gcttgggcta tatgtatgat acaggaactg
     1921 gtgtacctca gaattccgat acagccatgg tatggtataa aaaagcagca gagcaaggca
     1981 actctaatgc ggctttggct attggttata attatgatac cggtacagga gtgaaaaagg
     2041 ataagactca ggctctcaac tggtatgcaa aagcagctga cttaggtaat gccagcgcac
     2101 aatataatct tggtttgatg tatgagcaag gggatggtgt tcctaaagat tatcagaaag
     2161 cagcagagta tttcgagaaa gcagcgaatc aaggtcatgc caaatcgcaa ttggagttag
     2221 gttatttata tgactctgga aaattaggta aatcggatct gcaaaaggct gctttttggt
     2281 atcaaaaaag cgcagatctg gggaatgcca atgcgcaatt taatttagct gatatgtatt
     2341 tttacggtga tggggtcggt aaaagccttg agcaatccgt atactggatg caaaaagcag
     2401 ctgagcaagg gtatggtaag gctcaaaatc aattaggtat ctattacaga gacggtattg
     2461 gtgttgctgc tgatcctgta aaagcttatg cctggtttac agcagccaag aataatggct
     2521 ttgaaaaagc ggcgagcaat gccagtgatc ttgagaaatc tatgaaccca gaggatctga
     2581 gtaaggcgag aactttagga caacaatata cggataatta caaggccaaa aaataaatgc
     2641 ctgttacctg ttttcaagag ctagggatac tgcatttgtg ggctatggga gtgtgcgaga
     2701 gaattctcgc atacactcgc ccaaaaatag aaaaatataa aattaatttc accagaataa
     2761 agagacctct ttgaatttaa gaaagta
//
LOCUS       DASLOE010000257         2701 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.258, whole genome shotgun sequence.
ACCESSION   DASLOE010000257 DASLOE010000000
VERSION     DASLOE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.258"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..239
                     /locus_tag="U6309_002860"
     CDS             <1..239
                     /locus_tag="U6309_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN5506269.1"
                     /translation="VSRLQLINQFESAPESTLFNQNTLAAVLDCSVQLLERNRWAGQG
                     VPYLKIGRTVRYRKSDILNYLQQQCTYNVLQDAV"
     gene            318..2072
                     /locus_tag="U6309_002861"
                     /pseudo
     CDS             318..2072
                     /locus_tag="U6309_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
     gene            2312..2623
                     /locus_tag="U6309_002862"
     CDS             2312..2623
                     /locus_tag="U6309_002862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506270.1"
                     /translation="MSVQNHKEVYKDFVIEYQLINRENLLIEGRAIIHGPICSTASTK
                     STKSDLDFLKFNNENDALKILKQMAEKRIDDASDVHIFEGEASLIKGNKCCACGKIVQ
                     N"
BASE COUNT          820 a          489 c          585 g          807 t
ORIGIN      
        1 atgtatcacg cttgcaactt atcaatcaat tcgagtctgc tccagagtcc accctattta
       61 atcaaaatac acttgccgct gtcttagatt gctcagttca attgcttgaa cgtaatcgtt
      121 gggctggtca gggtgtgccc tacttaaaaa ttggtcgtac ggttcgatat cgcaaatcag
      181 atattcttaa ttatcttcaa caacaatgca cttataacgt ccttcaagat gctgtttagc
      241 aattctttga ttaagtcttg tgattgattg gaaggtggtg gaacaccttc ctgtgaacgt
      301 tcttggagaa aaagaaaatg aattcgaatc aaatatatca tgtggatttt aaacagtcta
      361 aacgcattaa atcctttcca tatgcaagtg gcatgtttca attaacctct gatggggtaa
      421 tttttattgg taaggataaa gacaataatg agctccctcc tcgatggata tgctcctcct
      481 tgtatgttgt agctaaaact cgtgatgctc atagcggtga atggggtcga ctactcgaat
      541 gggtcgatga cgatgaagtg aaacatcaat gggctatgcc tttggcttta ttacaagggg
      601 attccagcga tgtaaggcga gagctggcac gattggggtt gactatttcg cctagtaaaa
      661 cagctcgtga cttgttagca tcctatattc aggtttttcc agttgaagag cgtgctcgtt
      721 gtgttgataa attgggttgg tataaagacg tatttgttac tgctaataaa gccatagggc
      781 aatccagtga ccaaatcgtt tttcaaaatg ccaatagtct tgaacccgca ctgtctgttg
      841 cgggcagttt agaggactgg cgaaattcta tcgcacgatt ggcagatggt aattctcgct
      901 tggtatttgc tatttccgca gcctttgcac caagccttgc taatcttgtt ggtgaagatt
      961 ccggaggctt tcatttcaga ggggcatctt cttctggaaa aacaaccgct ttaaaagtag
     1021 cggcttctgt ttggggtaag ccggacggct atatacgctt gtggcgaagt accgctaacg
     1081 gattagaagg attagcagcc cttcataatg atggtttatt aatcctcgat gagctgagcc
     1141 agatggatcc taaagaagct ggagaatgtg cttatctact tgccaatggt caaggtaaaa
     1201 cacgagcctc tcgttgtggt acagcaagac aatccatgcg ctggtcattg ctctttttat
     1261 ctgcaggtga ggagtcatta acttcgttaa tggcgaaagc tggacaacga tgtaatgcag
     1321 gtcaagaaat tcgtttagca gatattgaag cagatgctgg tgcagaaatg ggtttatttg
     1381 agcaattaca tgatcatatc aatccagcct ctatgtcact ctcattaaaa gaagcggcca
     1441 gccaatatca tggtgctgtt ggtctagcat ggctccataa aattgtgcag catcgagtgg
     1501 agttgatccc cctactcacc aacaaaatcc agcaatttgt aactaaggtc actaaacccg
     1561 aacattcggg tcaaattcaa cgagtagcaa gacgttttgc tttggttgcc atggctgggg
     1621 aaatggcgag tcattttgaa ttaactgatt ggaaacgagg caccgcctgt caagctgcgg
     1681 aaaactgttt taaagcttgg cttgaagatt ttggagaaca tggtaaccga gaagatcgag
     1741 ccatactatc gcaagttcgt gctttctttg aaaaagaagg ggcaagtcga tttgaaaacg
     1801 aaaatcatcc caacagcgaa cgactttata accgagcagg cttttttcat acagatgacg
     1861 aaggttttcg tatttttatg gttttatccg aagtctacag gaaggaaatt tgccaaggat
     1921 ttgaacctaa aatggtgaat aaagtgctta ttaacgctag ttggatagtt ccgggaaatg
     1981 acagtaaaac atcataaaaa cgacgaatta aaggggttgg tattccaaga tgttatattt
     2041 tcacggataa agtatggtct gatgagtttt aattgtccca ctcgttccac tattaatttt
     2101 gatagtggaa cacctaaagt ccagtaacta aaaggatgtt ccacttgtcc catttgttcc
     2161 acgtattttt agttataagt aaaaacctat taggttgtat tggctttaaa gaaaatgatt
     2221 ttcccttata atgacttcat ctgagatagg ctagacttgt aatagtagtc ctaaactgat
     2281 tttcacattg tcatatcaat aggagcattt aatgagtgta caaaaccaca aagaagtcta
     2341 caaagatttt gtaattgaat atcaactcat aaaccgtgaa aatttgttga ttgaaggtag
     2401 agccataatt catggcccaa tttgctcaac tgctagcaca aaatcaacta aaagcgacct
     2461 tgatttctta aaatttaata atgaaaatga tgcattaaaa attcttaaac aaatggcaga
     2521 aaaaagaatt gatgatgcaa gtgatgtcca tatttttgag ggagaagctt ccctaattaa
     2581 aggaaataaa tgttgcgctt gcggaaagat cgtccaaaat tagttaaaac caaaatactt
     2641 ggcaaataaa agtaccaaaa ggcctgctat tatcgctgat attaaagggt tttgacttat
     2701 a
//
LOCUS       DASLOE010000258         2673 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.259, whole genome shotgun sequence.
ACCESSION   DASLOE010000258 DASLOE010000000
VERSION     DASLOE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.259"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            93..1532
                     /locus_tag="U6309_002863"
     CDS             93..1532
                     /locus_tag="U6309_002863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506271.1"
                     /translation="MKTIPTSMKNKDICFAALIFNLRNNIFVPEEIREQLDYEEIYYE
                     AMLANPAALKDIPDDMKSKIINRFSHEEFFQLIQKYHFNCLPYLPDEWVEKIKREVLP
                     KVRTVVVYDPARYDREIRMSCQLYASRPQYQDQTIVINSEDLNHLLKTLSKVSEHSSI
                     KLVINGHSSYRSEQLAGLSSQSIVHYLEKYPIIHDLVLSGCSTVKMEVTAQEKEMIKE
                     FIFLINQKSIENNRSPEEIIDELVANGVFEKGHPEYKEYKKFVPFLQSITVTPEEIEK
                     NLKESLMKLVIDELEKSKKISHSITIKAIPSPLNVNETYEKSTRGFVVWNKELYKEGK
                     TGLDSDLRKLEYRNYFFNTIKPPVMAKIYIAKERITKKGENKLNGRNEKAYIIVTKGN
                     SAPELHHYDPAAKIISKLDLKENHSHELLSIVMQTIGLENIKQGINLFNLDDRNQKEK
                     LDDIILSIEDGTNKQELTSAKSIRYHITK"
     gene            complement(1626..2027)
                     /locus_tag="U6309_002864"
     CDS             complement(1626..2027)
                     /locus_tag="U6309_002864"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEN5506272.1"
                     /translation="MAYRKMLKSKIHRACVTQADLDYEGSITISPELLKAANILPYEA
                     VNVWNITAGTRFETYAITGEKGSTDICVNGAAAHLVTPGDLVIIASFTQILEEDCAAH
                     EPTVVFVDQFNRLKEIRPERIGVKNKIPCSA"
     gene            2363..>2673
                     /locus_tag="U6309_002865"
     CDS             2363..>2673
                     /locus_tag="U6309_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HEN5506273.1"
                     /translation="MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGA
                     LTQPLLQKLNRLTAIEIDTDLQSYLTCLPVSQGKLNLIPADALTVDFCQFGPHLRVVG
                     N"
BASE COUNT          925 a          456 c          503 g          789 t
ORIGIN      
        1 ttattgtatc gaaagatata ttacccctaa tacttaacga tagtgaaacg atgggggtca
       61 tcgtacaagc tctacgttgt ggggaaattg aaatgaaaac aattcccacc tctatgaaaa
      121 acaaggatat ttgctttgca gcacttatat tcaatcttag aaataatatt tttgttccag
      181 aggaaatccg agaacagctt gattatgaag agatttacta tgaggcaatg ttagcaaatc
      241 ctgccgcctt aaaagatatt cctgacgata tgaaaagtaa aattatcaat cgattttctc
      301 atgaggaatt ttttcaactt attcaaaaat accattttaa ttgtctaccc tatttgcctg
      361 atgaatgggt agaaaaaata aaaagggagg tattgcctaa agttaggacc gttgtcgtct
      421 atgaccctgc tcgttatgat cgggaaatac gaatgagttg tcaattatat gcaagcaggc
      481 ctcaatatca agatcaaacg atcgttatta atagcgagga tttgaatcat ttattaaaaa
      541 cgttatctaa agtatccgag cattccagta taaaactggt gatcaatggt cacagcagtt
      601 ataggtcaga gcaattggct ggattgagta gtcaatccat cgtgcattat ctagaaaaat
      661 atcccattat tcatgatctc gtcctatcag gttgtagcac ggtaaaaatg gaagtgactg
      721 ctcaagagaa agagatgatt aaggaattta ttttccttat aaaccaaaag agtattgaaa
      781 ataataggtc accagaagaa attatcgatg agcttgtggc gaacggtgta tttgaaaaag
      841 gccatccaga atataaagaa tataaaaaat ttgtaccatt ccttcagagc attacagtaa
      901 cgccagaaga aatagaaaaa aatttaaaag aatcgttgat gaagctggtc attgatgaac
      961 ttgaaaaatc aaaaaaaatc tctcattcca ttactataaa agccattcct agcccactta
     1021 atgtaaatga aacatacgaa aaaagtaccc gaggctttgt cgtatggaat aaagaactat
     1081 acaaggaagg gaaaacaggg ctcgattcag atttaaggaa attggaatat agaaattact
     1141 tttttaatac aataaagcca ccagttatgg caaaaattta tattgctaaa gaaagaatta
     1201 ccaaaaaggg tgagaacaag ctcaatggaa gaaatgagaa ggcttatatt attgtaacta
     1261 aaggaaacag tgcgccggaa cttcatcatt acgatcctgc tgcaaaaata attagcaagc
     1321 tggatttaaa agaaaatcat tcacatgaat tattatcaat agtgatgcaa acgatcggat
     1381 tggaaaatat taaacaaggc attaatcttt tcaacttgga cgatcgtaat caaaaagaaa
     1441 aattagacga tattattctt tcgatcgaag atggtacaaa taaacaagag ctaacaagtg
     1501 ctaaatcaat acgttatcac attacgaagt aacttctgca tcatgtgaga gttttgcatt
     1561 agcctttccc tggattaagc tttacctaat ccaggctatg atgaacttgg tgttggtaat
     1621 caatcttatg ccgagcaagg tattttattt ttaactccga ttctttcagg tcggatttct
     1681 ttcagacgat tgaattgatc aacaaaaacc acagtgggtt catgtgccgc acaatcctct
     1741 tccagaattt gcgtaaaaga ggcgataatg accaaatcac cgggggttac caaatgggct
     1801 gctgctccat tcacgcaaat atcagttgaa cctttttcac cggtaatggc ataagtttca
     1861 aatcgggtcc cggccgtgat attccaaaca ttaacggctt cataaggcag tatattcgca
     1921 gcttttaaaa gctctggtga aatagtgata ctcccttcat aatccaggtc cgcctgcgtg
     1981 acgcaagcac gatgaatttt tgatttaagc atttttctat aagccattat ttacttccta
     2041 aatttgagcc tggaaaaagc aattgatcaa atacgactaa gtctatttga tacatggctt
     2101 ttttcagatt gaaataatta aaaaaaatag ataagaatat caaccttggt cattgtggtt
     2161 gaattttacc ccatattttg gagtttacgc tatataaatt tgtaaagttg agagagatga
     2221 atttgagttg gtgagtattg ccggggtttg ctggcacttg acactaacct acggtaaaat
     2281 taacaggctt ctccaatggg gtatcccttg tgatgtcaac aagattactg aattaatttt
     2341 atcctaacat aggtgataga aagtgaggca tagcccacgt aaacgttttg gtcaaaactt
     2401 cttacaagac aaatatatta ttaatgaaat tctacgcgct atcaatcctt tggcggatga
     2461 taatatgctg gagattggcc ctggtttagg cgctttaact cagccattat tgcaaaaact
     2521 gaatcgatta acagccattg aaatcgatac ggatttacaa agctatttaa cctgcttgcc
     2581 cgtatctcaa ggcaaactga atttaattcc tgctgatgcg ttaaccgttg acttttgtca
     2641 attcggcccc cacttaagag tagtgggtaa ttt
//
LOCUS       DASLOE010000259         2652 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.260, whole genome shotgun sequence.
ACCESSION   DASLOE010000259 DASLOE010000000
VERSION     DASLOE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.260"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..421
                     /locus_tag="U6309_002866"
     CDS             <1..421
                     /locus_tag="U6309_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506274.1"
                     /translation="TSEGISNLYEKSELVKWFSRDPRNKSDPVGLGLNVTISQYKTPT
                     PKELLDFVIKSTGFAEEVFIEHYKKPQVNKVPPEEELLLEKPDYTRRNEIDSPSHPQQ
                     RSCLSLFLYSIFGCKGKRYGTQEKPESLTSNYLPSNH"
     gene            625..2040
                     /gene="ankD"
                     /locus_tag="U6309_002867"
     CDS             625..2040
                     /gene="ankD"
                     /locus_tag="U6309_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector AnkD/LegA15"
                     /protein_id="HEN5506275.1"
                     /translation="MLTPPPDSKITTTDRSLDKLSIPMDMLKQMNESTMEQTKLDELR
                     KKMSLQTEILNKAKADNDMFFRLLIELMSLKLQGELFKEQLSKISKESGYDSVQSALI
                     QATNSEGQSPLQYALQKQDFTTAKYFLDNGAKAGPIEKAVFEIALDSKAAKEFGFPPL
                     PPEKEKLHPVKNFGLVLGIKTTSVDGTPSQFGHIAPTYQLMTDSVSDFAKSHPGNKNF
                     QEIANAFHFSNEASAFKFSTPQRNPEAGQDLSRRIQSGELTTIPVSCKGHAMGLSYVP
                     DGPGSKSGYLVYTNRGLGSKSNEHGTHIFRIEDSSKITSEFINNMTNGHSNGASHDEI
                     MSQIKAAAGNKDPIHHIKQKGQKNDNCTIANSKSNIEGILLCQKAREVGGFDKLTESD
                     KESVKKEYKEFTKHMRVEKVNELAKALKENPQDPDLNNLTKEYLKQHPNADPKLKQTL
                     ETALKQASESSMTLSQPGKTI"
     gene            complement(2052..2459)
                     /locus_tag="U6309_002868"
     CDS             complement(2052..2459)
                     /locus_tag="U6309_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN5506276.1"
                     /translation="MDLAADKVDILYLEDDEVDIQSVERVFHKISSLIKIEIAKSGNQ
                     ALDMLYGRNKENKIHPKLILLDINVPKMNGIEFLKELRADSSFTDIEVFVLTAAYTSK
                     DKLAFEPLNIRGHLIKPLDYGEAIKLFWILQSM"
     gene            complement(2522..>2652)
                     /locus_tag="U6309_002869"
     CDS             complement(2522..>2652)
                     /locus_tag="U6309_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN5506277.1"
                     /translation="TTGIGLTIVKKIVESQNGKIQVDSELGKGARFSFTWPKVPHQ"
BASE COUNT          945 a          530 c          447 g          730 t
ORIGIN      
        1 aacatcagag ggtattagta atctttacga aaaaagtgaa ttagtaaaat ggttttcaag
       61 agaccccaga aataaatccg accccgttgg acttggttta aatgttacta tatctcaata
      121 taaaactcct actccaaaag aattgcttga ttttgttatt aaatcaacag gatttgctga
      181 agaggtgttt attgaacatt ataaaaaacc ccaggtaaat aaagttcctc cggaagaaga
      241 gcttttgctt gagaaacccg attatactag acgaaatgaa atagatagcc catcccatcc
      301 acagcaaaga tcctgcttga gtctcttttt gtacagtatt tttggttgta aaggaaagag
      361 atacggaaca caggaaaaac ccgaatctct aacatcaaat tacctaccgt ctaatcatta
      421 aaattaataa agacaaattc ggtcttttta gacagttttt ttgttatgta caggacttac
      481 gcccccaaag atcaagacaa aaatagggga gtttagataa actcaattat tgaattaaaa
      541 acatttttta ataaggagat tgggggtttt gtaagtcctg atgtataata atgatatagt
      601 tcaactaatc aatcagggtg ttccatgtta actcctccac ctgactcaaa aattacaacc
      661 accgatagaa gtttggataa attaagtatt ccaatggata tgcttaaaca aatgaatgag
      721 tcaacaatgg agcaaaccaa actcgatgaa ctacgaaaaa aaatgtctct acaaacagaa
      781 atactcaata aggcgaaagc agacaatgat atgttttttc gtttgcttat cgaattaatg
      841 tcattaaaac tccaaggtga attatttaaa gaacaattaa gcaaaatttc taaagaaagt
      901 ggttatgaca gcgtacaaag cgcattaatt caagctacaa actcagaagg tcaaagccct
      961 ttacaatacg cgctgcaaaa acaagacttc actacggcaa aatattttct ggataacgga
     1021 gcaaaagcag gcccgataga aaaagccgtc ttcgaaatag cccttgacag taaggcagct
     1081 aaagagtttg gctttcctcc actcccacct gaaaaagaaa aactgcatcc tgtcaaaaat
     1141 tttggtctgg ttttgggtat aaaaacaacc agtgtggatg gcactccctc tcaattcggc
     1201 catatagcgc ctacctatca attgatgaca gattctgtca gtgatttcgc caaatctcat
     1261 cctggcaata agaattttca agaaatagcc aatgccttcc atttttcaaa cgaggcatct
     1321 gccttcaaat tcagcacacc gcaaagaaac ccggaagcag gtcaggatct ttcaagacgc
     1381 atccaaagcg gcgaactcac cacaattcct gtaagctgta aaggacatgc tatgggtttg
     1441 tcttatgtac ccgatggccc tggctccaaa tctggctacc tggtatatac caatcgcggt
     1501 ctgggatcga agagcaatga acatggcacc catattttcc gaatcgagga ttccagtaaa
     1561 atcacctcgg aatttatcaa taacatgacc aatggacatt cgaatggtgc ctctcatgat
     1621 gaaattatgt ctcaaattaa agcagctgct ggaaacaaag atcccattca ccatatcaaa
     1681 caaaaaggac aaaaaaatga caattgcaca atagccaact ccaaatccaa catagaggga
     1741 atactattat gccaaaaagc cagagaagtg ggagggttcg ataaattaac tgaaagcgac
     1801 aaagaatctg ttaaaaagga gtataaggaa tttaccaaac atatgagagt tgaaaaagtc
     1861 aatgaattag ccaaagccct taaagaaaat cctcaggatc ctgatttaaa taatttgacc
     1921 aaagaatatt taaagcaaca tccaaacgcc gaccctaaac taaaacaaac cttggaaact
     1981 gcattaaaac aagcttctga aagtagtatg acattaagcc agccaggaaa aaccatttaa
     2041 tactacagta cctacatgga ttgtaatatc caaaatagtt taattgcctc gccataatca
     2101 agcggtttaa tcaaatgacc tcgaatattc aaaggctcaa aagccagctt atccttgctg
     2161 gtataggcgg ccgtaagcac aaacacctct atatccgtaa aggaagagtc ggctcttaat
     2221 tctttcaaga actcaatacc attcatttta ggtacattga tgtcgagaag aattaacttg
     2281 ggatgaattt tattttcttt atttcgacca taaagcatgt ccaatgcctg atttccactt
     2341 ttagctattt caatcttgat taatgaactg attttatgga atacgcgctc aacagactga
     2401 atgtcaacct catcatcttc caaatacagg atatctactt tatcagctgc gaggtccatc
     2461 attattctcc gtgagacaat attttatttt ttatttctaa attaattata gacctaaacc
     2521 tttattgatg aggaacttta ggccatgtaa atgaaaatct tgcgccttta cctaactcag
     2581 aatccacctg aattttccca ttttgagatt ctacaatttt ctttactata gttaacccta
     2641 tcccggtagt tt
//
LOCUS       DASLOE010000260         2627 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.261, whole genome shotgun sequence.
ACCESSION   DASLOE010000260 DASLOE010000000
VERSION     DASLOE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.261"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(52..903)
                     /locus_tag="U6309_002870"
     CDS             complement(52..903)
                     /locus_tag="U6309_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN5506278.1"
                     /translation="MAMNTKVILITGCSSGIGFDAVFALKNRGHRVIASCRKTEDVQK
                     LLNKGVEALLLDVSNPISIQSAFAQLLIMTSGHLDVLINNAGYGQVGALEDISRDILR
                     QQFETNVFGLQDLTNLVIPVMREQGQGRIINISSILGVVSMPFRGAYNASKYALEGLS
                     DTLRLELSGSGINVITIEPGPINSRFRDNAVDLSLQQIPMEKSYFKNQYRNMLSNFKQ
                     KKADSFFTRDTDAVIKKLVHAVESRKPKPKYPVTFPAYFLIGLKRFFTTRLLDRFILF
                     LSRKELG"
     gene            1057..2565
                     /gene="ravZ"
                     /locus_tag="U6309_002871"
     CDS             1057..2565
                     /gene="ravZ"
                     /locus_tag="U6309_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavZ"
                     /protein_id="HEN5506279.1"
                     /translation="MKGKLTGKDKLIVDEFEELGEQESDIDEFDLLEGDEKLPGDSEL
                     DKTTSIYPPETSWEVNKGMNSSRLHKLYSLFFDKSSAFYLGDDVSVLEDKPLTGAYGF
                     QSKKNDQQIFLFRPDSDYVAGYHVDAKSDAGWVNDKLDRRLSEISEFCSKATQPATFI
                     LPFVEMPTDITKGVQHQVLLTISYDPKSKQLTPTVYDSIGRDTYSESLSSYFKGKYRT
                     TCDEILTQSIEKAIKSTDFTLGKFTRAAYNHQNRLTEGNCGSYTFRTIKEVISSSAQG
                     TEVKIPGSGYITSNSYLTSQHVQDIESCIKYRNLGVVDIESALTEGKTLPVQLSEFIV
                     ALEDYGKLRSQQSEKSMLNFIGYSKTAKLTAVELLIGILNDIKGKNEISESQYDKLVK
                     EVDCLMDSSLGKLVQFHLKNLGAESLQKLVLPCVKFDDTIDDFVTIEKDELFDVPDIT
                     GEELASKKGIEQGALDKEALLKQKQIKTDLLDLREEDKTDLKKPLHGGIKVK"
BASE COUNT          872 a          478 c          475 g          802 t
ORIGIN      
        1 aattatcaac accaacttat gactatttta taatattacc aataagttgc tttaacccag
       61 ctccttcctg gataaaaata aaataaacct atctaataaa cgggtagtaa agaatcgttt
      121 taaaccaatt aaaaaataag ctggaaaagt tacagggtac ttgggttttg gtttccttga
      181 ctccacggca tgaaccagtt tttttatcac tgcatcagta tcccgagtga aaaacgagtc
      241 tgccttcttc tgtttaaaat tgctcagcat atttctgtat tgattcttga aataactttt
      301 ttccattggt atttgttgta aagataagtc caccgcatta tcacgaaatc gactatttat
      361 agggccaggc tctattgtta tgacattaat acctgaacca gacaattcca gtcgaagtgt
      421 gtcacttaag ccctccaatg catatttgga ggcattataa gcaccgcgaa atggcatgct
      481 aactacgcca agtatagagc tgatattgat tatgcgtcct tgtccttgtt ccctcattac
      541 aggaataacc aaattggtca aatcttgcaa accaaataca ttcgtttcaa attgttggcg
      601 aagaatatcc cgagaaatat cctcaagagc acctacctga ccgtatcctg cattattaat
      661 caatacatcc aggtgacccg acgtcataat taacaactgc gcaaatgcac tttggattga
      721 aatagggtta cttacatcca acaagagagc ctcaacccct ttatttagta atttttggac
      781 atcctctgtt tttctgcagg atgcaatcac tctatgtcct ctatttttca aagcaaaaac
      841 agcatcaaaa cctatcccac tggagcatcc tgtgatcaaa ataacttttg tattcatcgc
      901 catgacctat gcctaatttt taactatcgt actaagctat ctatcgaatt accactatta
      961 tgataattat ttgttcaatt tttgacttat atttcacttt ttcctaaaaa tcatttgcta
     1021 aacttttaat atggaaatga cagtttagag gtcaaaatga aaggcaagtt aacaggtaaa
     1081 gacaaattaa tagtggatga gtttgaagaa ctgggagaac aggaatccga tatcgatgaa
     1141 tttgatcttc ttgaaggtga tgagaaattg cctggcgact cagagcttga caaaactaca
     1201 tctatttatc ctcccgaaac cagctgggaa gtgaataaag ggatgaacag ttctcgtttg
     1261 cataaactat acagtttatt ctttgacaaa agttccgcct tttatcttgg cgatgatgtt
     1321 agtgtattgg aagacaagcc tttaacaggt gcctatggtt ttcagagtaa aaaaaatgat
     1381 caacaaattt ttttattcag accggattct gattatgttg caggatatca cgttgatgca
     1441 aaatcagacg ctggatgggt aaatgacaaa ctcgatcgtc gtttatctga gattagtgaa
     1501 ttttgtagta aggccactca acctgcaacg tttatacttc cttttgttga aatgcccact
     1561 gacattacca aaggggttca acaccaggta ttgttaacta ttagctacga tccaaaaagt
     1621 aaacaactca ctcccactgt ctatgactcc attggacgag atacctactc tgaatcgtta
     1681 tcatcctatt ttaaaggcaa atatagaaca acttgcgatg aaattttgac ccaaagtatt
     1741 gaaaaagcta ttaagagcac tgattttact cttggtaaat ttacacgagc tgcttataac
     1801 catcaaaacc gcctcaccga aggcaattgc gggtcttata ccttcaggac gattaaagag
     1861 gttatttcat cctcagcaca aggtactgaa gtaaaaattc ctggctcagg atatattact
     1921 tctaacagtt atttaacaag tcagcacgta caagacattg aaagttgcat taaatataga
     1981 aatttggggg ttgttgatat cgaaagtgct ttgactgaag gtaaaacatt accggtacag
     2041 ctttctgaat ttattgttgc cttggaagat tatgggaaat tacgaagtca acaatctgaa
     2101 aaaagcatgt tgaattttat tggctacagc aagacagcaa aattgaccgc tgttgagctg
     2161 ctaataggca ttttaaatga tatcaaaggc aaaaatgaaa tcagtgaatc tcaatatgat
     2221 aaattagtaa aggaagtcga ttgtttaatg gattcatctt taggaaagtt ggtacaattt
     2281 caccttaaaa atttaggcgc tgaatcacta caaaaactgg tacttccgtg tgtcaagttt
     2341 gatgatacga tagatgattt tgtgactatc gaaaaagacg aactattcga tgtacccgat
     2401 attacagggg aagagcttgc ctctaaaaaa gggattgagc aaggtgctct ggataaagag
     2461 gctttactaa aacaaaaaca gattaaaacg gatttgttag accttcgcga agaagataaa
     2521 accgatctga aaaaaccatt gcatggtggc attaaggtaa aatagtgtga attgaattca
     2581 actatgtgat taataggcag aaggtttttt aatgcgctga taatttc
//
LOCUS       DASLOE010000261         2579 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.262, whole genome shotgun sequence.
ACCESSION   DASLOE010000261 DASLOE010000000
VERSION     DASLOE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.262"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(117..632)
                     /locus_tag="U6309_002872"
     CDS             complement(117..632)
                     /locus_tag="U6309_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5506280.1"
                     /translation="MFNVSIRKATSLDAEAIASVHVHSWKKAYQQFIPEIVLNSLSIP
                     ERTQLWNELLNNGVTVLVLEIDNQIIGFASICTFRDGIEDNSKGEISAIYIHPNFWHQ
                     GFGKKLSLAALKTLKELGYTEALLWVLSDNHQARSFYEGMGFELNDKSKLAEFYDGGA
                     LLKEVLYKKKL"
     gene            1180..1302
                     /locus_tag="U6309_002873"
                     /pseudo
     CDS             1180..1302
                     /locus_tag="U6309_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          824 a          475 c          428 g          852 t
ORIGIN      
        1 ataactttga tttttgacca gagttttaca accagttaag atccaaatag aaaagctata
       61 ttcttttcct tacacagtcc acaaacccat cattttaaga atatgatatg ttgaggttat
      121 aatttctttt tatacagcac ctcctttaat aaggctccac catcgtaaaa ctccgcaagt
      181 tttgatttat catttaattc aaagcccatg ccttcataaa agctacgcgc ttgatgatta
      241 tctgataaaa cccaaagcaa agcttcggta tagcctagtt cttttagggt ttttaacgca
      301 gcgagagaca atttcttacc aaatccttga tgccaaaaat tggggtgtat ataaatagca
      361 ctaatttccc ctttagaatt atcctcaatg ccatcgcgaa atgtacaaat actcgcaaac
      421 ccaattattt gattgtcaat ttccaataca agtactgtta cgccattatt tagtagttca
      481 ttccatagtt gggtgcgctc aggaattgat aaactattga gtactatttc tggaataaat
      541 tgttgatagg cttttttcca agaatgaaca tgtactgatg caatcgcctc cgcatccaaa
      601 gaggtagcct ttctaataga aacattaaac atgaattgat gcccccttta tttagctttg
      661 ggtctcgaat aatttaattt gttttaccat ttttatgata aaagggacag tataccaacc
      721 aattaatccc tctgtaacat gactcaatcc taaaaaacag aagaaagggt caaacctttc
      781 cctttgcctc aaaagtttta ccctcgctaa ttctggactt gcttaactcc ttttgcaaaa
      841 aaattaatac attgggaaat aattattagg aattaatcca tggcttattg tcttttaggg
      901 atagaggcaa aacgcaagat ccgacccttg ggttgatcac aatcgaacat aaaaacttag
      961 gtatcttcat gaaaatcaaa atccttaatg actctcaaca cgacaaaacc aaatatttat
     1021 ccaagtctta tcccaatcgg acaaatatac gcttaaaacc tataattgac tattttaata
     1081 agggaattta gcaagtaagt ttgggcattt tgtttgtaat tttcataagg ccaattatta
     1141 ataaagctca gatttaactg ctttttgact ctaccccaac aaagcccttt taaatcatta
     1201 gttgccttgg gaaaaaccct cgattcaagg gaagaagaaa ttgctcgtat gtggcaattt
     1261 agtaaatcaa aaggcatcac tgaaggattt catcggaaaa tgaattgaag gcatgggttt
     1321 aaaatcatca ggttgctcac cacgctgtac tatgtcgagt tgataatccg tatcttgttt
     1381 tgcatcggct tcaaactctc acaaacagct aagcgaatca cctaaaaatc gtattggttt
     1441 catttgttat ttttatatct gttttgatat aatttgcaag tttgaacggc aagactttac
     1501 agtaacagga tccattagtc tcttgctcta aagctacata ggaaaagcgg ggatttcagc
     1561 ctgcgcttgc tatgctaaag tacacttaaa gttcagagat atgtttatag atattcaaac
     1621 cattaaaagg aattttagtt tttataccaa cgttatactg attttttctc ttatattagg
     1681 cttggcaatt atgtatggtt attggtcaac gttcggtatt aatatatttc catttccagg
     1741 attatttgat ctcataagat tttctgcata tccattattg atactttaag ttcaactatt
     1801 gttactttac tgactgtttc ttgttgcaaa atttgcaaca actgaatctt tatctaattc
     1861 attggtgtta aatatattac ttaagtgtcg tgaaataacc gacttgtcag aatggaaaat
     1921 aatattaatg aacgttttcc tgacgctatt tttatttatt ttccaataag agttgtaatg
     1981 gggtcacttc cgaaaatata ggctaattat gtctgaatat ccaaggaggg atgggggatt
     2041 tttccgacca caaacctatt cccttgcttt tagcttgttc ctgaagcgca tataacgctg
     2101 gatcaccttt ggtaaagaca gtattattca agaaaacaat agtatagttc agcaaaaaat
     2161 tatcgcattt ggcaggatgg ttgctaatgc tgtgatgttc cacagatgcc aatacaacta
     2221 atgtgctaaa ccagcttgct gctgaaggag tcaagtatag tatagatgct ctatgtattt
     2281 tatcagctta cttcaggacg aatcaccctt tcggtaacat ttatttatga ggcaacaggc
     2341 tatttaacat cagttatgct ccaaatcatc ctgtagtgac aaatagtctt gaatttagta
     2401 cccagcaagc ctgcgaaaac gcaggtaaac aagttcaaga gctcacaaga acccgcaatg
     2461 gctctgcagt gcccgatttt atttgtctta aaaaataagg gaatggtgct gccgtagtgc
     2521 ctatacataa gaagccatgt atttttatat tataaatcaa taagttaatt tgggcgacg
//
LOCUS       DASLOE010000262         2575 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.263, whole genome shotgun sequence.
ACCESSION   DASLOE010000262 DASLOE010000000
VERSION     DASLOE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.263"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..630
                     /gene="pdxH"
                     /locus_tag="U6309_002874"
     CDS             <1..630
                     /gene="pdxH"
                     /locus_tag="U6309_002874"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEN5506281.1"
                     /translation="LADIRRDYGELQLSEESAENDPISQFKLWFDDVLLNEKNDPTAM
                     VLSTVDEKGYPDSRVVLLKGLENGNFIFYTNYQSAKAMQIQKNPYAALNFYWPQMARQ
                     VRVRGRVKKISSEQSDAYFSSRPLKSQFSAIVSPQSQEILDRISLEDALNQLIEEYGQ
                     KPVVRPENWGGYMIIPDEIEFWQGRDNRLHDRIHYYRHGHEWTHRRLAP"
     gene            1243..1614
                     /locus_tag="U6309_002875"
     CDS             1243..1614
                     /locus_tag="U6309_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmission trait enhancer LetE"
                     /protein_id="HEN5506282.1"
                     /translation="MNDTTSLLSHLRLRFNIEHPSYEECYIFGYECALAEVPEEDNPF
                     REGSQEAEQWLEGWWAGIYGEKPLFDLNNIEEDILLDKTTAANDQQYHHKNSFLSLVF
                     EIGGAIAASAIVGYQLLELVA"
     gene            complement(1685..2074)
                     /locus_tag="U6309_002876"
     CDS             complement(1685..2074)
                     /locus_tag="U6309_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEN5506283.1"
                     /translation="MNKLNIAIKEAFESSGSSKEANKVYLEFIKANFIIPIESNSDDS
                     EPRVLFLEENNHVFLPVFTDMKYLDAWASEISQHIKLLKLSGVDLLKGLGEKVTVCLD
                     IGSELYKEFNPSEIERMRSMVLKLFKN"
BASE COUNT          828 a          422 c          483 g          842 t
ORIGIN      
        1 cttgcggata ttagacgaga ttatggtgag ttgcagctca gtgaagaatc cgctgaaaat
       61 gatcccattt ctcaattcaa actatggttt gatgatgtat tactaaatga aaaaaatgat
      121 cccactgcaa tggttctgtc tacagtagat gaaaagggct atccagattc cagagttgtc
      181 ttattaaaag gattagagaa cggtaatttt atcttttata ccaattatca aagtgccaag
      241 gcaatgcaaa tccaaaagaa tccttatgca gcacttaatt tttattggcc acaaatggca
      301 agacaagtac gtgttcgtgg tcgagtaaaa aaaatcagta gcgagcaatc cgatgcttat
      361 ttctcctcca gacctttaaa aagtcagttt agtgcgattg tttctcccca aagccaagaa
      421 attctggata ggatttcttt agaagatgct ttaaatcagt tgattgaaga atatggccaa
      481 aaacccgtag tcagaccgga aaactggggt ggatatatga taatcccgga tgagattgag
      541 ttttggcagg gtagagataa cagattacat gatcgaattc attattaccg gcatggtcat
      601 gaatggacgc accgtagatt ggcaccttga ccggcagttt ttgtcatcat tcctgccggc
      661 atcattttac tattgagata tagaaggatg agttttcatg gaaggttggt taatggttga
      721 attcttttga ataagcacat gggattcgca aattttccca gatttataaa ccaggactcg
      781 cgaaaaattc caatctcttt gttaaaaaca tgttgagctt tgaccttaga ataatgtgtc
      841 cgttatatca atattctgtt ggggatagat gggttagagc gggataattt atcgttcttg
      901 acggttgtta ttttaagcaa tatagacatt tattaagcat aaaagtccat gaaaaaataa
      961 gataaaaaaa ttggcctaat acttgcttct ctattaataa aatcaataag ttaatgaagt
     1021 atcgtgttaa agaaccaaaa gtaaaaagat ttttgtcaag ataatgtaaa tatttgatct
     1081 tcgaaggcag ggggcaaatt aaagtcgtaa aattgattta agtgtgggag ttacatgcac
     1141 taaaaagcgg ttctgcacca attgcgttaa taattataga cttaggagta aatgagttga
     1201 aaagcggaaa tatattcagg atacataaaa gaggctatca tcatgaacga tacaacatca
     1261 ttattatcac atcttagatt acgatttaac attgaacacc caagctatga agaatgttat
     1321 atttttgggt atgaatgtgc attggcagaa gttccagaag aagataatcc tttccgcgaa
     1381 ggttctcaag aagcagaaca atggctcgaa ggatggtggg ctggtatata tggagaaaaa
     1441 ccattatttg atttgaacaa tatcgaggaa gatattcttt tagataaaac cacagctgca
     1501 aatgatcagc aatatcatca taaaaacagc ttcctatctt tagtctttga aattggtggt
     1561 gcgatagccg cttccgcaat agtaggctac caattacttg agctcgtagc atgatcatcg
     1621 cgttcaattg aagagtataa tgtaatgcct cttcaattga atgggagtgt ttcttgtatt
     1681 gagttcagtt tttaaataat tttaaaacca tcgacctcat tcgttcaatt tcagatggat
     1741 tgaattcttt ataaagctca ctgccaatat caaggcaaac tgtaactttc tcacctaaac
     1801 ctttcagcag gtcaacccct gataatttaa gtaatttgat atgttggcta atttcacttg
     1861 cccaagcatc gaggtatttc atatcagtga ataccggcaa aaaaacatgg ttattttctt
     1921 ccaaaaataa tactctgggt tcactatcat cagaattgga ttcaatagga attataaaat
     1981 ttgccttgat aaattcaagg taaactttat tcgcttcttt tgagcttccg gaagattcaa
     2041 aagcttcttt aatggctata tttagtttgt tcatcagggg tagatctcat gtctgtatag
     2101 tatcttgaaa atttgccttt taaacttgtt cagagtaacc aacttccatt catgaaaata
     2161 tatgaggatt caattttaac aggacaaggg cataccaatt tcggtcaatt atgagccctg
     2221 gtactttata aatttgaatc tatttttgtc atccgcacag gcggaacgac tcgaatgttt
     2281 cactatataa tcaaaatatg attttgcctt actctcttta aaacttcttg gtgaaccctc
     2341 ggggattatt catttttgtt ttgagttcat ccactggata tccgaaaact ccaatctctt
     2401 ggttaaaaaa tgtttttaat tcagtcattt agtttgtcta aattctctca tttaaaacat
     2461 ttttttcctc gaccttggct tttttcgtag gtcctggtct tttaatcatt caactcaatc
     2521 cagaggattt ttgttttgct ggatgacgat gagtcattaa aaattaggat tatat
//
LOCUS       DASLOE010000263         2516 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.264, whole genome shotgun sequence.
ACCESSION   DASLOE010000263 DASLOE010000000
VERSION     DASLOE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.264"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..812
                     /locus_tag="U6309_002877"
     CDS             <1..812
                     /locus_tag="U6309_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN5506284.1"
                     /translation="ISTLVALEVQKGKLNVTDYAVQYTPELSKTNGLPIDRVQIVHLA
                     THTASFPKQMEQFGVNKGNIQAFFARLKTWRPPVKIGTHYKYSNIGFGYLGYVLENAS
                     NETLPNLLKSNITKPLGMSHTFFNVPESLANYEAQGYRPKNKPAPYYTPANFLGGGAL
                     RSSAADMLVFLKANLGIQPGTASPELLSAMQYAQQPFFEVSPNFVMGLGWQRVKRGRH
                     IVITKNGANQGFSTFIGFSPAEKLGVVVLINQARGKATALGNQILNSLLGL"
     gene            1063..1500
                     /locus_tag="U6309_002878"
     CDS             1063..1500
                     /locus_tag="U6309_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506285.1"
                     /translation="MKKSNHVHSLLLLTAITFSPVIKASPVGLIVDIYCPTTQGGPNV
                     ITNFGDYIAGYGMENILSQNNPIYFKSISLAHDVPAQLGNYYNETTSYNSTTGQVTCS
                     YISNNPTEPRFAVAYTLTNGRGGSVQWQSGNSINIIFPVGLKS"
     gene            complement(1516..1653)
                     /locus_tag="U6309_002879"
     CDS             complement(1516..1653)
                     /locus_tag="U6309_002879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506286.1"
                     /translation="MNTLITRYVLLLGFALALSAGFLSISLANQFQEELLGDEEDSQA
                     F"
     gene            complement(1719..2012)
                     /locus_tag="U6309_002880"
     CDS             complement(1719..2012)
                     /locus_tag="U6309_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506287.1"
                     /translation="MNVNSENAQPGEAVTAEDYSRAMNFIGQNLLSSLTQSVEKLPPP
                     FRNRRLVCQALSAFLTNVIYKQFPEQPESCQQMLDDITRHVSMQLDKIPQPSK"
     gene            complement(2237..2428)
                     /locus_tag="U6309_002881"
     CDS             complement(2237..2428)
                     /locus_tag="U6309_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEN5506288.1"
                     /translation="MLYWALIFLIVAIVAGLFGFRGVASAATGIAKVLFFLFIVMFIV
                     LLVFSLLGGTPEPVVIVKP"
BASE COUNT          821 a          520 c          461 g          714 t
ORIGIN      
        1 tcattagcac tcttgtagcc ctggaagttc agaaaggaaa attgaacgta acagattatg
       61 ccgttcaata tacgcctgaa ctttcaaaaa ccaatggact accaattgac agggttcaaa
      121 ttgtgcatct ggcgacgcac acagcaagct ttcccaaaca aatggagcaa tttggagtta
      181 ataaaggcaa tatacaggct ttctttgccc gtcttaaaac atggcgtcct ccagtaaaaa
      241 taggaactca ttataaatat tccaatattg gttttgggta tttaggatac gtattggaaa
      301 acgcctcaaa cgagacttta cctaatctgt taaaatcgaa tattaccaag ccattgggaa
      361 tgtcccatac ttttttcaat gtaccagaaa gtctggctaa ctatgaagct caaggttatc
      421 gacctaaaaa caagccagca ccttattata cacctgccaa ctttctaggt ggaggagctt
      481 tacgctcttc tgcagctgac atgttggtat ttttaaaagc aaatttagga attcaacctg
      541 gcaccgcatc accagaatta ctttctgcga tgcagtatgc ccaacaacca ttttttgaag
      601 tcagcccaaa ttttgttatg ggattaggtt ggcaaagagt gaaaagaggg aggcacattg
      661 tgatcactaa aaatggagca aatcagggat ttagcacctt tattggtttt tctcctgcgg
      721 aaaaactggg agtagtcgta ttaatcaacc aagcaagagg caaggcaact gcattaggca
      781 accaaatact taatagcctt cttggtttat aattcataca ttggcacaaa agttaatact
      841 gtctttcata agcaagtcac acaacactta aaggctattt gtttgtttga gatagattca
      901 caggcattga caatcaaaat gtcgtgcaac atgattacct ttgtaccttt tctctgtatg
      961 gcttagctta caacgtggat atgatgattt taatctgaag caatggctaa cagaacaaca
     1021 ggtcaaacca gcaacttaaa ataaccgaaa aagggagttt cgatgaaaaa atccaatcat
     1081 gtacactcat tgttattgct taccgctatt actttttcac ctgttatcaa ggcaagccct
     1141 gtaggtctga ttgtagatat ctattgccct actacccagg gaggaccaaa tgtcatcact
     1201 aattttggag attacattgc tggttatggg atggaaaata ttttatcaca aaacaatccc
     1261 atttatttta aatccatttc actagcccac gatgttccag cccaacttgg aaattattac
     1321 aatgaaacga ccagctacaa cagcacaaca ggccaagtca cttgcagtta cataagtaat
     1381 aacccaacag aacctcgctt tgctgtggca tataccttaa ccaatggcag gggcggttct
     1441 gtccaatggc agtcagggaa ttcaatcaat atcatttttc cagttggtct aaaaagttaa
     1501 ccaaagttta ctggattaaa atgcctgact gtcttcttcg tctcccagca actcttcctg
     1561 gaattgattt gctagggaga tagacagaaa tcctgcactc aaagccaaag cgaaaccaag
     1621 cagaagaaca tacctggtaa ttagggtatt caagctgaaa cgcatgattt atttgagttc
     1681 aactcaggcc aaattagctg atacagatta ctaacgtttt actttgatgg ttgaggaatt
     1741 ttatccagtt gcatactgac atgcctggta atatcatcga gcatttgttg gcatgactca
     1801 ggttgctctg gaaactgttt ataaataaca ttagtcaaaa atgcagacaa agcctgacaa
     1861 actaacctgc gattacgaaa cggtggcggt aacttttcca cagattgagt taatgatgac
     1921 aataaattct gacctataaa attcattgcc ctggaataat cttcagcggt tacagcttcg
     1981 cctggttgag cgttttcact attcacattc attttttatc ctgtcagtgt gtgatcaagc
     2041 cggattatag agtctatctc gagctacttc aacgactgtt tcagctcttt ccataaaacc
     2101 agcacgatta tccattaaaa caacaaaata tctcattaac agaacttacg aaaaacccca
     2161 aggtcaagac aaaaagtgtt tttaataaag aggttggggg agcgtaagtc ctgattaaaa
     2221 ctcatttatg atgtagttat ggttttacaa tgacaacggg ctctggcgtg ccgcccaaaa
     2281 gactaaagac caaaagcaca ataaacatta cgataaagag gaaaaacaga actttggcta
     2341 tgcctgttgc ggctgatgcg acgcccctaa atccaaataa acctgcgaca attgcaacaa
     2401 taagaaaaat taaggcccaa tataacataa taaactccct gttttcagaa taatcctttc
     2461 ttcctgaatt gtttttaccg aagtaaaatc aagctgcccc tgcttatgat catctc
//
LOCUS       DASLOE010000264         2509 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.265, whole genome shotgun sequence.
ACCESSION   DASLOE010000264 DASLOE010000000
VERSION     DASLOE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.265"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..540)
                     /locus_tag="U6309_002882"
     CDS             complement(<1..540)
                     /locus_tag="U6309_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506289.1"
                     /translation="MSKLFIAGIGIKFLSHMTLEVQSVIQKCDCVIYLVNEPAIKRWI
                     DENSKKAISLDAIYFSFQERKEAYQAICQEVINIVTKNQNTCFIIYGHPLILSNSAEQ
                     LIKEIKEESLDLEIEILPGISSIDTLLCDLCIDPGNGGLQAFEATEFINTNYKINTNS
                     HLILWQIGVVGVKSIIQNDK"
     gene            complement(543..740)
                     /locus_tag="U6309_002883"
     CDS             complement(543..740)
                     /locus_tag="U6309_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506290.1"
                     /translation="MNTYEKLVYLATEPIDNKVVLCDKKGSKLKSSLAVQEELKDEFI
                     HADLSIDIRRACETHLSFVLR"
     gene            complement(760..1986)
                     /locus_tag="U6309_002884"
     CDS             complement(760..1986)
                     /locus_tag="U6309_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5506291.1"
                     /translation="MRNFFKNISALYLDVPKLTKTLLPIIILEHLVASFCLNISAYFK
                     KVNYFQYEQIGEFISCYYWGCLFGSLIGGALTLYYKTSKISGWGLMLLGVCLFGLMNS
                     ISLWLIAISMFLVGFIGTITATANLTSLIRSVKDNEKLKLKVISLDLIIFNLCFSFVT
                     FVLLDLSPLYILKYVQIMICLLVFVGIGSLIFYQDSIFNPPKLGSLGFKIILPERKQE
                     FMLLMSMVLFFGLIFSMVKVVFAPTLIERFGSNMMSVIAASINPWVVFLIQPLIVGRI
                     KNSNSIWFLGIGGLIVGLSYFMFGMVNSFELTAIVLVLLTFGEMMFGPLSKHFNIQLY
                     RSGQEGVATGIWRAVFLGSGAIGPGLSGYVAEYYGTYVVWDCCALLGLLCFVFSLYLR
                     KIQIKMPYSKLVLDNQ"
BASE COUNT          926 a          475 c          319 g          789 t
ORIGIN      
        1 tttatcattt tgaataattg atttaacacc gacaacaccg atctgccata aaatcaaatg
       61 gctattagtg ttaattttat aatttgtatt tataaattca gttgcttcaa atgcttgtaa
      121 accgccattt ccaggatcta tgcataaatc acacaaaaga gtatcgatgc tagaaatccc
      181 aggaagaatt tcaatttcga ggtctaaaga ttcttcttta atttctttaa ttaattgttc
      241 tgcagaattt gataatatta acgggtggcc ataaatgata aaacaagtat tttgattttt
      301 agtaactata ttaataactt cctgacatat agcttggtaa gcttcttttc tttcttgaaa
      361 actaaaatat attgcatcta atgagatagc ctttttgcta ttttcatcta tccatctttt
      421 tatcgctggt tcattaacca aataaataac acagtcacat ttttgtatga cagactgaac
      481 ctctaaggtc atatgagata aaaattttat tcctattcca gcgataaaca atttactcaa
      541 atttacctca atacaaaaga taaatgggtt tcacaagctc gcctgatatc tattgataaa
      601 tctgcatgaa taaattcatc ttttaattcc tcctgtacag ccaatgagga ttttaactta
      661 gatccttttt tatcacataa aactacttta ttatcaatgg gttcagtagc taaatagacc
      721 aatttttcat aggtattcat atacactcac ttacaatagt tattgattat ccaaaaccaa
      781 tttgctatat ggcatcttta tttgtatttt acgcaaatac aatgaaaata caaaacaaag
      841 caaccctaat aaagcacaac aatcccatac gacatatgtt ccataatatt cagcgacata
      901 ccctgacaag ccaggaccta tagctccact tcctaaaaat actgctctcc atataccagt
      961 ggcaacaccc tcttgtccag atctatataa ctgaatatta aaatgcttag acaatggacc
     1021 aaacatcatt tcgccaaatg ttaataaaac caatacaata gctgttaatt caaatgaatt
     1081 caccatacca aacataaaat aacttagacc tacaattaac cccccaatcc ctaaaaacca
     1141 tattgaattt gaatttttaa ttctccctac tatcaacggt tgtattaaaa atacaaccca
     1201 tggattaatt gatgcggcta ttactgacat catattgctg ccaaagcgtt caattaaggt
     1261 tggggcaaaa accactttta ccattgaaaa aataagacca aaaaacaaaa ccatgctcat
     1321 taaaagcata aattcttgtt tacgctcagg taatataatt ttaaaaccca aagatcctaa
     1381 tttaggtgga ttgaaaattg aatcttgata aaaaatcaat gatccaatcc caacgaaaac
     1441 tagcaaacaa atcatgatct gcacatattt caatatatac aatgggctaa gatctaataa
     1501 cacaaatgtc acaaatgaaa aacatagatt aaatataata agatccaaac taataacctt
     1561 taactttaat ttttcattgt ctttaacaga tctaattaag cttgtaagat tagcagtggc
     1621 tgttattgtt cctatgaagc ccactaaaaa catcgatatg gcaattagcc acaaacttat
     1681 tgagttcatt aggccaaata aacaaacccc taaaagcatt aatccccaac cagagatttt
     1741 gctcgtcttg taatatagtg ttaatgcccc gccaataagt gatccaaata gacaacccca
     1801 gtaatagcat gaaataaatt caccaatctg ttcgtattga aaataattaa cctttttaaa
     1861 atatgcagat atatttaaac aaaaggatgc aactaaatgt tctaaaatta ttattggcaa
     1921 taaagtcttc gttagttttg gaacatctaa atataacgct gaaatatttt tgaaaaaatt
     1981 tctcattcaa tcccttaaat ttatcgaata cagtgaggta tagtagtgcc gtgagaatac
     2041 atccaatgcc ttcgattaaa caacatgcgc aatataaaat taaattcgtc taatttcaat
     2101 aatattcgcc ggtgctgatt aattgattga taatttctct cataaaaaac aaaatggaac
     2161 catcgtgaga tttgcgtatt tgattacggc acagtacacc ataatatgcc atttacagtg
     2221 gttttcacgc agcgaatttt ttaataaaac caaggatggc tcataatccg ttggccctag
     2281 gataaagtcc tagtgggccc gccaataaaa tcagggggct attcctaaga catcggttac
     2341 aaattatatc ggaaacatag gtaacacttt aggccctaaa ggattacata aatttccaag
     2401 aagcagcgga ctaaaaaacg ccacttcccc atataagttg atcatctaca tcagcattca
     2461 tcctccagag ttttaatact cattattaaa atttcattaa gaattttaa
//
LOCUS       DASLOE010000265         2500 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.266, whole genome shotgun sequence.
ACCESSION   DASLOE010000265 DASLOE010000000
VERSION     DASLOE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.266"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            454..1128
                     /locus_tag="U6309_002885"
     CDS             454..1128
                     /locus_tag="U6309_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEN5506292.1"
                     /translation="MKILMVLTSHDMLGNTGRKTGFWLEEFAAPYFVFKDAGVELTLA
                     SPKGGQPPIDPKSDLPENQTPATRRFKHDELAKKELSQTVKLANVKAEDFDALFYPGG
                     HGPLWDLAESAESISLIESFYNSEKPVALVCHAPGVLRHVNYKGDPLVKGKRVTGFTN
                     SEEEAVQLTEVVPFLVEDELKRLGGLFEKKLNWQSFVIVDGHLITGQNPASSTAGAQA
                     LLKLLS"
     gene            complement(1248..1754)
                     /locus_tag="U6309_002886"
     CDS             complement(1248..1754)
                     /locus_tag="U6309_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5506293.1"
                     /translation="MTLKKPEPLNKDHNLTDFNSNYPELDEWLKKYAFQAKMSGSANT
                     FIVADNNLVVGFYSLTVGQVDVDEASERIKKGMGKYPIPVVILARLAVLHTYQGKGIG
                     KAMLKDAILKTLQIAEQGGVRALLVHAIDEQAEAFYKRFGFEPSPIRENQLLLLLKDA
                     KKVISEQR"
     gene            complement(1735..2106)
                     /locus_tag="U6309_002887"
     CDS             complement(1735..2106)
                     /locus_tag="U6309_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEN5506294.1"
                     /translation="MSNARKVDNKKTPTAREPRKVRRSQSRSVRAKTSRLGFRASESQ
                     AKIIRMASDISHKSLTEFITDSAYQAAEKIILDQRLFMVSCDIFTQFSEILDRPEQDN
                     EGLKDLFSSSSPWDLNDVKKT"
BASE COUNT          785 a          464 c          428 g          823 t
ORIGIN      
        1 acaatgaaag acgatgaaac accatattaa atcaagatta agtgttaatc ggcaattgct
       61 attttgttgt ctttagttgt ccttaattgt cagcaaataa acccgcaata aaccctttga
      121 tattctaatt taatgttcag cgattttttt tatgcttaat tttaatatat ccagttttcg
      181 aagtaattag actttatcgg gttttccgaa agattaaatc actacagctt ctttagtgga
      241 atcatacccc cttttatacc ttcttcgtca aaaccggcat taaccacatt gctgggaaca
      301 gcctctttta tccatttttt aaagttctct cttatatttc ccatgtcatg attcattgat
      361 taaattactt ccattggttt agatatatag cttagctgta tactatactg aaagaacttt
      421 tgtcatctta agcattctaa caaggagaag tgagtgaaaa tattaatggt gctgacctca
      481 catgatatgc tcggcaatac gggtcgtaaa acaggattct ggttggagga gtttgcagcg
      541 ccctattttg tatttaaaga tgccggtgtt gaactcacat tagcctcacc taaaggtggg
      601 cagcctccta ttgatccaaa gagtgattta cctgaaaatc agactccggc tacacggcga
      661 ttcaagcatg acgaactggc caaaaaagaa ctatcccaaa cggtcaaact tgcaaatgtg
      721 aaagcagaag attttgatgc gctattctat ccgggtggtc atggtccttt gtgggatctt
      781 gcagaaagtg ctgagtcaat cagtttaatt gaatcgtttt ataattcaga aaaacctgtt
      841 gctttagtat gtcatgctcc gggagtcctg cgacatgtca attataaagg ggatcctcta
      901 gtcaaaggaa aacgggtgac aggcttcacc aacagtgaag aggaagcagt ccagctcaca
      961 gaagtcgttc catttcttgt agaagatgag ttgaaacgat tgggaggtct attcgaaaaa
     1021 aaacttaatt ggcaaagttt tgttatcgtt gatggccatc taattacagg gcaaaatcct
     1081 gcatcttcaa ctgctggcgc ccaagcatta ctcaagctac tctcgtaacc ataaatgctc
     1141 tcactctctt gaattgcaat attcaagaga gtttctaatt aaaaactgat ttgaggatat
     1201 ccaattttca cgataatcag acttaatggc ttgaggtgga atttagctca ccgttgttca
     1261 gatattactt ttttagcgtc ttttaaaaga agtaatagct gattctctct tattggagaa
     1321 ggttcaaagc caaatcgttt ataaaaggct tcggcttgct catctatagc atgaactaat
     1381 aaagctctta cgccgccttg ttctgcaatt tgcaaagttt ttaatattgc atctttaagc
     1441 attgctttcc caataccctt accttgatat gtatgaagaa cagctaatct tgctaatatg
     1501 acaacaggaa taggatattt gcccattcct ttttttatgc gttcagatgc ctcatcaaca
     1561 tctacctgac ctactgtaag gctataaaaa ccaaccacaa gattattatc agcaactata
     1621 aaagtatttg cagaaccgga catttttgct tgaaaagcat atttttttaa ccattcatct
     1681 aattctgggt aattactatt aaaatcagtt aagttatgat ctttatttaa aggctcaggt
     1741 ttttttaacg tcatttaaat cccatggaga tgatgaagaa aataaatcct ttagcccttc
     1801 attatcttgc tctgggcgat ctagaatttc agaaaattgg gtaaagatat cgcaagaaac
     1861 cataaaaagt ctctgatcta aaataatttt ttcagcagct tgatacgcac tatccgttat
     1921 aaattcagtc aatgacttat gacttatatc agaagccatc cggattattt tggcttgcga
     1981 ttcagatgca cgaaaaccca atcgggaagt tttagcacgc acagaccttg attgcgatct
     2041 tctcaccttt cttggctctc tagccgtagg tgttttttta ttatccacct ttcttgcatt
     2101 ggacatgata tatctcaaaa taatctctat taattacatg ttagtacaat tgacgcacat
     2161 tatcaataaa aatagctttt cagtaaataa aacggcgggt tgttaactgc ataatagaca
     2221 tataaaacaa ggtaaaattc actataaggt ggtaattaat aaacaggcaa atcaaattaa
     2281 ttaagtatta aagcaataaa gaatattaac tagttatttt aaccgagctc ttttggaggt
     2341 taataagtag gccttctgtt ataagatgtt ctgtatcaaa aaccatgcta attttcttta
     2401 aagatccgat tctaaataca tacggtttcg ggcttaaaga cttaacattt ctataaattg
     2461 caattcttaa ctcttgctgc catttgtaag aatccaattt
//
LOCUS       DASLOE010000266         2450 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.267, whole genome shotgun sequence.
ACCESSION   DASLOE010000266 DASLOE010000000
VERSION     DASLOE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.267"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..145
                     /locus_tag="U6309_002888"
     CDS             <1..145
                     /locus_tag="U6309_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEN5506295.1"
                     /translation="NVSLSMGVISIGAMRVIDQQSAITAADNTLYQAKKAGKNQIKSK
                     SLG"
     gene            complement(457..843)
                     /locus_tag="U6309_002889"
     CDS             complement(457..843)
                     /locus_tag="U6309_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014840970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506296.1"
                     /translation="MKKWTVLAASSLLMVNAYANESFCGYKDFFHLGDKTHPGIYVVS
                     GYNDSDVVLQIVGPRSFVIRDGFDCRAGYAHVTVAYDNANWCVLDINDGPFMNHPVVS
                     ASCNGLRYINTTYDGFGSYSYSINIE"
     gene            1100..1699
                     /locus_tag="U6309_002890"
     CDS             1100..1699
                     /locus_tag="U6309_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg0375 family Dot/Icm T4SS effector"
                     /protein_id="HEN5506297.1"
                     /translation="MYSSLFLKKDLGHLPEKIIFLDFDGTVALPARKENGEYDIQPDG
                     SIGATHLNKEKFRRLVQFAIHTDIPLYFLSGRPDLQSCVDLMTDFIASVDGFHSGIGG
                     FKKDSLFFISKMIIEEGKLVRKEITTKAQVIQSVHHEKYSYLPKESILLVDDIQEYLD
                     PVRELGYNTLLANPESLEHFDEVEKFIGVTLQKTIAFNT"
     gene            complement(1739..>2450)
                     /locus_tag="U6309_002891"
     CDS             complement(1739..>2450)
                     /locus_tag="U6309_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein SdhA"
                     /protein_id="HEN5506298.1"
                     /translation="AFCNRHIGLQYTDKEYRTKLREYLLTFKESIIQRSKFAEDINLT
                     IKNLLKEKIKNFERDNFEKYYHLDAVRVALAQFKNYFSLSTAAIEQNNSTFESEETLA
                     KKTELINKLVDISEDGTLTPKERVDQISFHVKNPNFERIIMDYKQESYFSFTYLKQCI
                     LSLLEALCLYTPERRKLFNNLESSVKTQPKINELTKRFGLFAGNESATSTESRAITPV
                     SSVENNGTIELNQLAPSA"
BASE COUNT          755 a          396 c          486 g          813 t
ORIGIN      
        1 aaatgtgtcg ttgagtatgg gggtcataag cattggtgct atgagggtga ttgatcaaca
       61 aagtgccatt actgcagctg ataatactct ttatcaagca aaaaaagcag ggaaaaatca
      121 aattaaatct aaatcattag gctagcaaaa attgagagtc aattaaaatt attagatttg
      181 attataggaa gtatgagata agagaataga attttatttc tcttatgcca gagacggcct
      241 ctggattaat tacttttaac gtgagttatg gataagatag ctcattatat tgggttgttt
      301 gggctgagga gggatttatg tcgtctcgaa gccttgtatg aaatttaatg attcttgcca
      361 gggcttcgag aagatgctca tgtatctcct cagcccaaac ggtccatgga tatcaattgc
      421 ttcttatata atcgctatcc ataactcacg ttacttttat tcaatgttga tagaatagga
      481 gtaacttcca aagccatcat aggtggtatt gatgtaacga agaccattgc aggaagcact
      541 gacaacagga tgattcataa atggaccgtc attaatatca agtacgcacc aattggcatt
      601 gtcataagct acagtgacat gagcatagcc tgctcgacaa tcaaaaccat ctctgattac
      661 aaagctacgt ggtccaacga tttgtaatac gacatctgag tcattataac cactgaccac
      721 ataaattcca gggtgagttt tgtcacctaa atgaaaaaaa tctttgtacc cacagaatga
      781 ttcattagcg taggcgttca ccattaaaag gctacttgct gccagtactg tccatttttt
      841 catacgaaaa taccttctta ttgttattca ggaaatcgaa ttatagggaa tcgatggcta
      901 ttaaacaatc atcttgttct tttgtttttg aaaaacctgt tctgcaagtg atttctggga
      961 agggtaggaa atatttcctg ttaaagagat ggtcagttac cttgtttcga ttgatctaat
     1021 cggatacgct gaccagttga tattgagaat ctctacaata ttttgaaggg atagaaacat
     1081 taaattgtca ggaggaaaaa tgtattcatc attatttcta aagaaggact tggggcattt
     1141 gcctgaaaaa attatttttc ttgattttga cggcacagtt gctcttcctg caaggaagga
     1201 aaatggagaa tatgatattc agcctgatgg gtcaattgga gctacccacc taaataaaga
     1261 aaaatttaga cgtttagtac agtttgctat tcacactgat attcctttgt actttttgag
     1321 tggacgtcct gatcttcaat cgtgtgttga tttaatgact gattttattg caagcgtcga
     1381 tggcttccat tctggtattg gcgggtttaa aaaagattct ctgtttttta tcagtaaaat
     1441 gataattgaa gaggggaaac ttgttcgaaa ggaaataacg acaaaggctc aggttattca
     1501 atccgtgcat catgaaaaat acagttattt acctaaggaa tcgattttac tggtcgatga
     1561 cattcaagaa tacctcgacc cagtgcggga actgggttat aacactttgt tggcaaatcc
     1621 ggagagtttg gagcattttg atgaagtaga gaaatttatt ggggtaacat tacaaaaaac
     1681 tatcgcattc aacacctaga caaggttatg gtaaattaaa agattaaatc aaatattttt
     1741 atgctgatgg cgctaattgg tttaactcaa ttgttccatt attttcaaca gaagaaactg
     1801 gggtaattgc cctggattca gttgaggtag ctgactcatt gccagcaaat aatccaaatc
     1861 gtttggtcaa ttcattgatt ttaggctggg tttttacaga gctttccaaa ttattaaata
     1921 gttttcttct ctccggagtg tacaggcaaa gcgcttccaa taaggataag atgcattgtt
     1981 ttaaataggt aaagctaaag tagctttcct gtttgtaatc catgatgatt ctttcaaaat
     2041 taggattttt aacatggaaa gatatttggt cgacacgctc ttttggcgta agggttccat
     2101 cttcactgat atcaaccagt ttattgatga gctctgtctt tttcgccagt gtctcttcgc
     2161 tttcgaatgt cgaattattt tgctcaatgg cggcggtaga aagactaaag tagttcttaa
     2221 attgggctaa agcgactctt actgcatcaa ggtgataata tttctcaaaa ttatctcttt
     2281 caaaattttt aattttttcc tttagcaaat tttttatagt taaattaata tcttctgcga
     2341 attttgagcg ttgaataata ctttctttga atgtcagtaa atattctcta agttttgtgc
     2401 gatattcctt gtcagtgtat tgtaagccta tatgacggtt gcagaaagca
//
LOCUS       DASLOE010000267         2440 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.268, whole genome shotgun sequence.
ACCESSION   DASLOE010000267 DASLOE010000000
VERSION     DASLOE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.268"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(98..532)
                     /locus_tag="U6309_002892"
     CDS             complement(98..532)
                     /locus_tag="U6309_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEN5506299.1"
                     /translation="MNYYSILLTIKRLIIGLLPILLVACAHKQSHGPDHYVVRGKTYH
                     VMKSAKHYKAKGIASWYGSHARHQKTSTGERYNMYAMTAAHPTLPIATRVRVKNLNNG
                     RSVVVRINDRGPFLSNRLIDLSFAAAKKLGIKGIAPVEIEVV"
     gene            complement(843..2210)
                     /locus_tag="U6309_002893"
     CDS             complement(843..2210)
                     /locus_tag="U6309_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN5506300.1"
                     /translation="MAFLSFLKPAPYLDEIQDKNVVKRQYRYWRIRTFYAMYIGYALF
                     YFTRKSFTFVMPALQSTLGMTKTELGLIASILSLSYGISKFLSGILGDKSNPRYLMAI
                     GLILTGVFNILFGLSSTWWLFAIFWGLNGWFQGWGWPGCTKLLTHWYSQSERGRWWSL
                     WNTSHNVGGALIPLIVAVCAQYFGWRSAMFVPGIICILGGIFLINRLRDTPQSLGLPA
                     IEQYHEDFSGSANHHQEKTELSVKEILWNYVLCNQYIWILSISYLFIYIVRTAINDWS
                     MLYLTEVKEYSLITAGSCVIWFEVGGFFGNLVAGWSSDKIFQGKRNPINVLFTAGVFA
                     TLLLFTLTRAYTPFLDSLFLFFFGFFIFGPQMMIGMACAELAHKKAAATATGFAGFFA
                     YCLGAVLAGAPLGAIIKNYGWDNFFLTLFICCLIPLFLMLPLWHVKVNPKYRQSEFSG
                     KSEFA"
BASE COUNT          837 a          495 c          439 g          669 t
ORIGIN      
        1 acgaaattca ttgatgaata ggacgatttc ttaattttag attatttaat aagttaatca
       61 ggttgttagc ttgagcaagc tttacttctt agcattttca aacgacttca atttcaacag
      121 gagcaatacc ttttataccg agttttttag cggcagcaaa ggataaatca attagcctgt
      181 ttgataaaaa aggacctcga tcattaattc gaaccacaac cgatcggcca ttattcaggt
      241 ttttgacccg tactcgtgta gcaattggaa gtgttggatg agcggctgtc atcgcataca
      301 tgttgtagcg ctctccggtc gatgtttttt gatgccgagc gtgtgaccca taccaggaag
      361 caattccttt tgctttgtag tgtttggcag atttcatgac atgatatgtt ttccctctga
      421 ccacatagtg atccggacca tgagattgct tatgcgcaca agcaaccagt agtatgggga
      481 gtaaacctat aatgagtcgt ttaatggtca acaaaatcga ataataattc ataaagttat
      541 cttctacttc cgctacagat tttaaatata cgtaattcat agatcacgag aagcaaagat
      601 atttcttgca aagaaaatcg tcaataggca acacattttt atcatttgct ttcatcattg
      661 attagattga ttatacattt tttggatgaa tatgtaccaa caaattttgc tgtataacct
      721 cttatcctat cacaacaagg atacctctat accccagggg ctttcatgtg gaggtagcaa
      781 gagcaggtcg ggccaaggca gacccaacct actttttctg gattacccag agaagtcaat
      841 cactaagcaa actcggattt gcctgaaaat tctgattgac gatattttgg attgactttc
      901 acatgccaga gcggcagcat caaaaataat ggaatcaagc agcaaataaa taatgtgagg
      961 aagaaattat cccagccgta atttttgata attgcaccca aaggagcgcc tgccagaaca
     1021 gcgcctaaac aataggcaaa aaatccggca aagccagttg cagtcgccgc tgctttctta
     1081 tgtgctaatt ccgcacaagc cataccaatc atcatttgtg ggccaaagat aaaaaaacca
     1141 aagaaaaaaa gaaacaaaga gtcgaggaac ggtgtatatg ccctggttaa cgtaaacagc
     1201 aacaaagtgg caaatactcc tgcggtaaaa agaacattga ttgggtttcg tttaccttga
     1261 aatattttat cagaagacca gccggcaacc agatttccaa aaaaaccgcc aacctcaaac
     1321 catatgacac aactgcctgc tgtgattaag gaatactcct tcacttccgt taagtacaac
     1381 atgctccaat cattaatggc cgttcggaca atgtaaataa acaaataaga aatggacaat
     1441 atccaaatat attggttgca caacacataa ttccaaagaa tttctttgac tgagagttct
     1501 gttttctcct ggtggtgatt tgcggaacca gaaaaatctt catgatattg ctctatcgcg
     1561 ggcaagccaa gcgattgtgg agtatctctt aaacgattga ttaaaaaaat acctcctaaa
     1621 atgcagatga tcccaggcac aaacatggct gaacgccagc cgaaatattg cgcgcacaca
     1681 gcaacaatca aaggaattaa tgcaccacca acgttgtgtg atgtattcca aagactccac
     1741 caacggccac gctccgattg ggagtaccaa tgtgttaata atttagtaca tcctggccag
     1801 ccccaccctt gaaaccaacc atttaatccc caaaaaatag cgaataacca ccaggtggaa
     1861 gacaatccaa acaaaatatt aaaaacacct gtcaaaatca gtccgatcgc cattaaataa
     1921 cgaggattag atttatcacc tagaattccg ctcaaaaatt tgctgatacc ataacttaaa
     1981 ctcaaaatgc tggcgatcaa ccccagttca gttttagtca tccccaaggt gctttgcaat
     2041 gcaggcatca cgaaggtaaa actttttcgc gtaaaataaa acaacgcata ccctatatac
     2101 attgcataaa aggtgcgaat acgccaataa cgatattgcc gtttaacaac gtttttatcc
     2161 tgaatttcat caagatatgg agcaggtttt agaaaactca aaaatgccat gattgtccat
     2221 gatcatgaaa aaaacatgat tagaatgtaa ctcgcaaagt atgtcaaatc tttctaatga
     2281 aaatttgaca ggatcatacc tattgatgac tgaaataaaa atttaatgga attcttgtga
     2341 atttataaat aagctacgca gtttgtatta aaaaaggatt tattgtaatg aaagaaatag
     2401 ttagtttatt agaagaagat caaatcgact ttgctaatca
//
LOCUS       DASLOE010000268         2402 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.269, whole genome shotgun sequence.
ACCESSION   DASLOE010000268 DASLOE010000000
VERSION     DASLOE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.269"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            53..676
                     /locus_tag="U6309_002894"
     CDS             53..676
                     /locus_tag="U6309_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5506301.1"
                     /translation="MSLSSVKKVYNIYSSFYDVLFGSIFHQGRSLCANKVNKNAGLNA
                     SVLELGVGTGLSLPFYRPDLNITGIDISEKMLEKAEKRIVKKKLTTHIDLKIMDAANL
                     EFPDNHFDFVVAMYVASVVPDIDTFLKEISRVCKPSGEIIFVNHFASEKPILRFFEKQ
                     LSHIDNFVGFNSNFSIHSILNYKEFKLLETQKVNLFGYWKLLHCKIN"
     gene            complement(705..1826)
                     /locus_tag="U6309_002895"
     CDS             complement(705..1826)
                     /locus_tag="U6309_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN5506302.1"
                     /translation="MNVLTSWIKAWSQKFFPNQKKNENKEEEADLTQEKKVRPNADFY
                     ENYFSLVAAGTRLKLIEAMFGVNLFSLFENKAWVFEKDIIEKLQLMPIRARKWLHLLS
                     SEHFLIQIKVNGQQAYQLPEEFIELIHSEEFPAMEMFFKTWLVSAEENLTDVLRFGKV
                     KTSVSWPPKTQQEAEWLEDWMTRTADQPTYCLLQSINFKKVNKFLDVGGGDGTMACTF
                     VKKYPHLKAAVYNLPMSAQMARKNIESRKLNHRVRVIEGDFIEEDAFPVGYDLILFTR
                     VLFDWDDQVCRKLLRMAYQALPKNGLVGICEFYKEDNNDICLASEYRYIFHDDFGVNV
                     MKRASEYQNMLEKIGFTIVQPHKVKAMHFYYCSLILARK"
     gene            complement(2113..>2402)
                     /locus_tag="U6309_002896"
     CDS             complement(2113..>2402)
                     /locus_tag="U6309_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506303.1"
                     /translation="LQKLNNAKYLINSLYMAIVDHWKIYDDWPSETKEDSTDYNPLEA
                     LAAYLCEEDKKKLCKIIGRNYLEPSSEEEFDDIEESEWTNSELGSTSARTM"
BASE COUNT          760 a          469 c          377 g          796 t
ORIGIN      
        1 taacatagag attgggtttt ttcgtaagtc ctgtctaaat ggagtattgg ttatgtcgct
       61 ctcttcagta aaaaaagttt ataatattta ttcttctttt tatgatgtcc tttttggatc
      121 tatctttcat caaggccgct ctctatgtgc taataaggtt aataaaaatg ctggcctgaa
      181 tgcttctgtt ttagagcttg gggttggcac agggttatct ttaccctttt accgtccaga
      241 tttgaatata actggaatcg atatttcaga aaaaatgtta gaaaaggctg aaaaacgtat
      301 cgtcaagaaa aaattaacaa cccatattga tttaaaaatt atggatgccg ctaatttgga
      361 gtttccagat aaccattttg attttgttgt tgccatgtat gttgcgtctg ttgtccctga
      421 tattgatact tttctaaaag aaatctcacg tgtttgtaaa ccatcaggcg aaataatatt
      481 tgtgaatcat tttgcttctg aaaaacctat cctgcgcttc tttgaaaaac aactgtcgca
      541 tattgataat tttgtaggat ttaactcgaa cttttctatc cattccattt taaattataa
      601 agaatttaaa ttgctggaaa ctcaaaaagt caatcttttt ggctattgga aattattaca
      661 ctgtaaaata aattgaagct tcacaaaaat aaagataaga atttttactt tcgagccaat
      721 atcaaagaac aataataaaa atgcatagcc tttactttat gcggttgaac gatagtgaaa
      781 ccaatttttt ctaacatgtt ctgatattct gaggcccttt tcatcacatt cacaccaaaa
      841 tcatcgtgaa aaatgtaacg atattccgag gcaaggcaga tgtcgttgtt atcctcttta
      901 taaaattcgc aaataccgac taatccattc tttggcaagg cctgataagc cattcttaat
      961 aattttctac acacctgatc atcccaatca aacagaactc ttgtaaataa aatcagatcg
     1021 taccctaccg gaaaggcatc ttcctcaata aaatccccct caatcacacg cactctatgg
     1081 tttagtttcc tcgattcaat attttttctt gccatctgcg cagacattgg caaattgtag
     1141 actgccgctt ttaaatgtgg atatttttta acaaaagtac acgccatagt tccatcacca
     1201 ccacccacat ccaaaaattt attgaccttt ttaaaattga tagattgtaa taaacaatag
     1261 gtaggctgat cagctgttct agtcatccaa tcctcaagcc actctgcttc ttgctgagtt
     1321 ttcggaggcc aggaaacact cgtcttgact tttccaaagc gcaatacatc agtcaagttt
     1381 tcttctgcac taacaagcca ggtcttaaaa aacatttcca tggcagggaa ttcttcactg
     1441 tggattaact caatgaattc ctcgggtaat tgataagctt gctgcccatt aaccttaatt
     1501 tgtattaaaa aatgctccga actaagaaga tgcagccact tcctggccct gattggcatt
     1561 aattgcaatt tttctataat atccttttcg aaaacccatg ccttattttc aaataaagaa
     1621 aataaattga cgccaaacat ggcttcaatc agtttaagtc tggtaccagc ggctacaaga
     1681 gaaaaataat tttcataaaa atcggcatta ggcctcacct ttttttcttg ggttaaatca
     1741 gcctcttcct ctttattctc attttttttc tgattgggga aaaatttctg tgaccatgcc
     1801 tttatccacg atgttaaaac gttcatccga caatattctt ttaagagtac tattaatatg
     1861 agaatagaac aggacttaca aaaaaaacca ggttcaaggc caaaaatgtt tttaatgagg
     1921 gagtttagat aaactcaatg accaagttaa actctttttt aacaaggaga ttggggggcc
     1981 tgtagaaaaa aactcgttca gctggtttcc tcaaataaca caatgggaac gataagtatg
     2041 caaatgcaga tatgatccag cgtgatagtc cgcacttgct actttaaaaa gatgactgtc
     2101 agtcaggctt ttttacatgg ttctggcaga agtgctccct aactctgaat tcgtccattc
     2161 gctctcttca atatcatcaa actcctcttc tgacgaaggt tccagataat ttcgtccaat
     2221 aattttacaa agtttcttct tatcctcttc acataaataa gcagccaaag cttcaagagg
     2281 attatagtca gtagagtctt ctttagtttc agatggccaa tcatcataaa ttttccaatg
     2341 atcaacaata gccatgtata gactgttaat cagatattta gcattattta atttctgcaa
     2401 aa
//
LOCUS       DASLOE010000269         2336 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.270, whole genome shotgun sequence.
ACCESSION   DASLOE010000269 DASLOE010000000
VERSION     DASLOE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.270"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            50..433
                     /locus_tag="U6309_002897"
     CDS             50..433
                     /locus_tag="U6309_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEN5506304.1"
                     /translation="MYIQKLKEMFAQMVIKKNANLIPEYYHGEFLLYTNDIVTGYDEF
                     LSSHQQYYATEIQYQVEYDEETFLEQGEKIAGRVFITTTRPNEPAKRIEVILIAQYKD
                     EKIYRLWELTYPDWSKLPAFSNQLT"
     gene            659..1213
                     /locus_tag="U6309_002898"
     CDS             659..1213
                     /locus_tag="U6309_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HEN5506305.1"
                     /translation="MKGIIFNEFLNFVEKSESYTLVDQIIMDSHLKSHGAYTSIGTYS
                     PKELFQLVKALAMKNGKPTSVILQEYGEYLFEVFAKKYPQFFREKKSVFQFLEALETH
                     IHFEVKKLYDHTELPHFECQYHSQNQMEMIYTSSRPLADFAEGLIRGCIKYHKENMTI
                     VRENLPAKTGFKVRFVLTKGDPDE"
     gene            1206..>2336
                     /locus_tag="U6309_002899"
     CDS             1206..>2336
                     /locus_tag="U6309_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEN5506306.1"
                     /translation="MSDYEVLLRHLDREIRARKEAEEISEKKISELYRFAQRLEESLK
                     AQKEISKKLKEKTKALKQQIAKTEEERHAKSTLQTILESAIEYSIIAINLKGVILVWN
                     EGARRNYGYEAQELVNKESIEILHTVEDIQSGKVRKFFQTVRREGKAEEIFQRVRKDG
                     SQFTASVTMTIRYDSQGKKVGYVIISKDITEIKEFEEKLIKSNQNLEEFSYIISHDLK
                     APLRAIIQLATWIEEDCGNKLDSLSRKNLQLLRERACRMSGMVDGLLQYARAGRVNME
                     VEWVDTKELIQEIIDILNHDKQFTIIVHESMPQLPTAKLLLSQVLSNLIANSMKHHHC
                     KTGTIEIGVLDKGTFYEFFVADDGPGIEPVYFEKIFRIFQTLK"
BASE COUNT          844 a          369 c          459 g          664 t
ORIGIN      
        1 tgctaaataa ttgttgtata attttgaaaa attaatttga gagtagaaaa tgtatataca
       61 aaaattaaaa gaaatgtttg ctcaaatggt catcaagaaa aacgccaatc ttattccaga
      121 atattaccat ggggagtttc tgctctatac caatgatata gtgacaggct atgatgaatt
      181 tctaagctcg catcaacagt attatgcgac tgaaattcaa tatcaagttg aatacgatga
      241 agagaccttc cttgagcaag gtgaaaaaat agctgggcgg gtatttatta ccaccactcg
      301 acctaatgag ccagccaaga gaatcgaagt cattttgatt gcccaatata aagatgaaaa
      361 gatttatcga ttatgggagt taacttatcc agattggtcc aagttacctg ctttttcaaa
      421 tcagctaacc tagacgattc ttttctcctc caaacagggg gatatcagaa aagtagttgg
      481 tcaagatttc tccgctttac atggtgtagc gccacgcagg agcgagcaat gtttcatggt
      541 ttaatcaata tgatcaaaaa aatgcaaaac attcctgctt gcgtcaagat tatattattg
      601 ataacaaatt tacgactata cttatagtat taatcaattg atataaaaca tcttgtctat
      661 gaaaggaatc atattcaacg aatttctcaa ttttgtagaa aaaagtgaat cctacaccct
      721 ggtagatcaa attattatgg atagtcattt gaagtcccat ggtgcctaca cctctatcgg
      781 tacatactct cccaaagaat tatttcaatt ggttaaagcg cttgctatga aaaatggcaa
      841 accaacatca gtgattttac aagaatatgg tgagtatttg tttgaggttt ttgcaaaaaa
      901 atatcctcaa tttttcaggg aaaaaaagtc agtatttcaa tttttggaag cgcttgaaac
      961 acatattcat ttcgaagtga aaaaattgta tgaccatact gaactacccc attttgaatg
     1021 ccaatatcac agtcaaaatc aaatggaaat gatttacact tcttcgcgtc ctttagccga
     1081 ttttgcggaa ggtttaataa gaggttgtat taaataccat aaagaaaaca tgactattgt
     1141 tcgtgaaaat ctgcctgcaa aaacaggctt taaggtaaga tttgtattaa caaaaggcga
     1201 tcctgatgag tgattatgaa gtattgttac gccatcttga tagagagata cgcgcaagga
     1261 aagaagctga agaaataagt gaaaaaaaaa tatccgaatt ataccgtttt gcccaacgtc
     1321 tggaagagag tttaaaggct caaaaagaaa taagtaaaaa attaaaagag aagaccaagg
     1381 cattaaagca gcaaatcgct aaaacagaag aagaacggca tgccaaaagt actttacaaa
     1441 ctatattgga aagcgcaatt gaatattcaa tcatcgcaat caatttgaaa ggcgtcatct
     1501 tggtttggaa tgaaggggcc agacgaaatt acggctacga ggctcaagaa ttagtgaaca
     1561 aagagagcat tgagattttg catacagtgg aagatatcca gtcaggtaaa gtaagaaagt
     1621 tttttcaaac agtacgtcgg gaaggaaagg cagaagaaat atttcaaaga gtcagaaaag
     1681 acgggtccca gttcactgct tccgttacta tgactattcg ctatgattcg caaggaaaaa
     1741 aagtagggta cgttattatt tcaaaagata ttaccgaaat taaagaattt gaagaaaagt
     1801 tgattaaaag taaccagaat cttgaagagt tttcatacat tatttcccat gacctaaaag
     1861 cgccgctaag ggcaattatt caattggcaa cgtggattga agaggattgc ggaaataagt
     1921 tggattctct ttccagaaaa aatcttcaat tgctcagaga acgtgcctgt cgtatgagcg
     1981 ggatggtaga tggcttatta caatatgcgc gggcaggcag ggtcaatatg gaggtagaat
     2041 gggtagatac taaggagtta attcaagaaa taatagatat tcttaaccat gataaacaat
     2101 ttactattat agtccatgaa tccatgcccc aactgccaac ggccaaatta cttctaagtc
     2161 aggttttgtc taatctgata gcaaatagca tgaaacacca tcattgtaaa acgggaacaa
     2221 ttgaaattgg tgttttggat aaaggcacat tttatgagtt ttttgtggca gatgatggtc
     2281 caggtattga gccggtgtat tttgagaaaa tattcagaat atttcaaact ttaaaa
//
LOCUS       DASLOE010000270         2318 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.271, whole genome shotgun sequence.
ACCESSION   DASLOE010000270 DASLOE010000000
VERSION     DASLOE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.271"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            54..770
                     /locus_tag="U6309_002900"
     CDS             54..770
                     /locus_tag="U6309_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEN5506307.1"
                     /translation="MKILYVPFPRNQAGDLKSMVELWKKNDEQFSPDRIEIIYYNDDI
                     NYDELDEKIAIYICAHGSDDDNVTKLFNHSNPLVAESLDIKEVAERFERDILPISYWI
                     SAVHLYCCGSKNKNQMMAELLGHSLLRPEKPIYHYSGSVSIVDEYGKQWSFANHVKIP
                     VHLVAKRTFILNFFDEEQPHRAFVKKAFQGKTYEELLAKKEDSFFAKVKEKRASVLLS
                     KRLGKKSQDGEENSLLRKGN"
     gene            906..1547
                     /locus_tag="U6309_002901"
     CDS             906..1547
                     /locus_tag="U6309_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506308.1"
                     /translation="MKEQFLQPKDLNTLLRSVEKPIQDLLDLNLKTFKSVSYVTPIEL
                     FNVLKPEEILEKNLNVFIQNSQKAMNYMLNVFQIMEHHWINIYDQMAENPNEQKANPA
                     IDKANRKVDTSTPKSSASKKAVTAKGKTKPAAVQSKSKTSKAKPKISSPTKVKINTQE
                     RPAAVKQQSQMSLSSLQKDMEKQGTNTPVELKNSVAILKNETKDIVPSNNKLK"
     gene            complement(1775..>2318)
                     /locus_tag="U6309_002902"
     CDS             complement(1775..>2318)
                     /locus_tag="U6309_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN5506309.1"
                     /translation="AIEQFYNMYPVPLIVINNKPDEESSVAMLEAGADDFMTKPIYPR
                     ELHARIGAISRRVIRAQQKVDHGKEVLMFANWRLYPASRQVFGENNEELQLSAGEYDL
                     LLTFVQQPQRVLDREFLLQITKNTDLVPFDRRIDVQISRLRQKIEIDKKKPALIKTIR
                     NGGYMFTARVVTLKESDATL"
BASE COUNT          812 a          381 c          439 g          686 t
ORIGIN      
        1 tattttaacg catttgtgct aaaataatgc ctttagaata tgaaatagag cgtatgaaga
       61 ttttatatgt acctttcccc agaaatcagg ctggtgattt gaaatcaatg gttgaacttt
      121 ggaaaaaaaa cgatgagcaa ttttcccctg atcgcattga aataatttat tacaatgatg
      181 acattaatta tgatgaattg gatgaaaaaa ttgcgatata tatttgtgct catggttcag
      241 atgatgacaa tgtaacaaaa ctttttaatc attccaatcc attagttgca gaaagcctcg
      301 atataaaaga agtggcagaa cgctttgaaa gagatatttt acctatatcg tactggatat
      361 cagcggttca tctttattgc tgtggaagca agaataaaaa tcagatgatg gctgaattat
      421 tagggcattc attgttaaga ccagaaaagc ccatttatca ctacagtggg tcagtttcta
      481 tcgttgacga atatgggaag caatggagtt ttgcaaacca tgttaaaata cctgtgcatt
      541 tggtagctaa aagaacgttt attctaaatt tttttgatga agagcaaccc catagagcct
      601 ttgttaaaaa agcatttcaa ggcaagacgt atgaagagtt attagccaag aaggaagatt
      661 ctttttttgc gaaagtgaaa gaaaaaagag cttcagtact tttatccaaa cgattaggca
      721 aaaagagtca ggatggtgaa gaaaactctt tgttgcgaaa gggtaattaa agtttttacc
      781 tttgatacat tttagtttgc gatgcgaatg atttatggag ttacaagaaa atggcattta
      841 atatttgaca aattatattt ttaagctaat cttgtttttg tgcattgcaa catatggaga
      901 gtgtaatgaa agagcaattt ttgcaaccta aagatttaaa tacgttgctt cgatctgttg
      961 aaaaaccaat tcaggatttg ttggatctca atcttaaaac attcaaaagc gtgtcttatg
     1021 ttactcctat cgaattattc aatgttttaa agccagagga aattctggaa aagaatctga
     1081 atgtattcat tcaaaatagc caaaaagcaa tgaattacat gttgaacgta tttcaaataa
     1141 tggaacatca ttggattaat atctatgatc agatggccga aaatccaaat gaacagaagg
     1201 ctaatccagc aatagacaaa gcaaatcgca aagtagatac cagtactcca aaatcatcag
     1261 ccagtaaaaa agccgttaca gcaaaaggta aaacaaagcc tgctgcagta cagtcaaaat
     1321 caaaaacaag taaagcgaaa ccgaaaatca gcagcccaac aaaagttaaa ataaatactc
     1381 aagagcggcc tgcagctgtc aaacaacaat cgcaaatgtc gttatcttct ttgcaaaaag
     1441 atatggaaaa acagggtact aacacgcctg ttgaattgaa aaactcagtt gcgatattaa
     1501 aaaatgaaac aaaggatata gtgccttcta ataacaaact taaataaact ggtgctttat
     1561 cctgttttcg ttttgagcgc ttttctgaga agtagcaagc gatacttgat aaatccgggg
     1621 atacccaaaa gctagacatg gtagggtgca atgccagcaa gtaaaggata aggcgggcat
     1681 tgtgccaata aagcaaacat cagttcatag tttttaaaat gcgctcaata aataagatga
     1741 acgaatttgt ctaaataagc cagataataa tcaattataa tgtggcgtca ctttctttaa
     1801 gggttacaac tcgagctgtg aacatgtagc cgccattgcg aatggttttg attaaagctg
     1861 gctttttttt atctatttcg attttttgac gcaaacgact aatttggaca tcaatccttc
     1921 ggtcaaaagg aaccaggtct gtatttttgg taatttgcaa taaaaattct ctatccagga
     1981 ctcgttgcgg ttgttggaca aaagtgagaa gcaaatcata ctcaccggcg cttaattgca
     2041 attcttcatt attctccccg aacacttgtc tggaagctgg ataaagtcgc cagttagcaa
     2101 acattaatac ttctttacca tggtctactt tttgttgcgc cctgattact cgacgagaaa
     2161 tagcaccaat tcgcgcgtgc agctcacggg gataaatggg tttggtcata aaatcatcag
     2221 caccggcttc aagcatggca acgcttgatt cttcatcagg tttattatta ataacaatta
     2281 atggcactgg atacatatta taaaattgtt caattgct
//
LOCUS       DASLOE010000271         2298 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.272, whole genome shotgun sequence.
ACCESSION   DASLOE010000271 DASLOE010000000
VERSION     DASLOE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.272"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..292
                     /locus_tag="U6309_002903"
     CDS             <1..292
                     /locus_tag="U6309_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEN5506310.1"
                     /translation="RDTTNALSENERLTNKLKQTASAQKHLAKAYNLNDRLYQTGIQS
                     YIETLNSKISLDKINISLNQDKLQELLSIVRLYQELAGGYRANLESEIKSSV"
     gene            527..2203
                     /locus_tag="U6309_002904"
     CDS             527..2203
                     /locus_tag="U6309_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M4 family protein"
                     /protein_id="HEN5506311.1"
                     /translation="MLKKTMFFILSISASSIFAADNVDLYQAPLNSINKYPILQTPKN
                     AIILKSSSAVIDNSLQKLNQTKEDNQMIVRYQQLYKGIPVIGAQVMITKGTDSGVQSN
                     DNAEVNGHLLENIELNTKPAISAQQAKEYAKKSYFQFSSQSNIQQETAELQIRPDHNN
                     QLKLVYLVSFKSVQQDGKPDWPFFVIDAQTGALIKQWNNIKNYLDTGPGGNEKVQEYW
                     YGKDGLPALDVTQNGSQCVMENSKVKLVNLHSQWDWENTINTPFEYVCNNNIEENING
                     GFSPGNDAYYFGHVIVGMYKDWYGLNALQHSNGAPMQLVMRVHFGQNYDNAFWDGQAM
                     SFGDGLDFYPLVSLDVAGHEVTHGFTEQHSGLEYHDQSGALNESLSDMAGQASRAYLL
                     EKNPQLYNKAYLQPNEVTWGIGETIVRDSYGKALRFMDYPSSDGSSADCLDKGIAQNN
                     GSYCAISYDEVVAYANTHIALPQERQSFIVHTASGVFNKAFYLMSKDMGIKNAYHIMV
                     VANTKYWTPTTDFKNGACGVIYAARDLNTDINKVKSAFGQVGIDTAGCVI"
BASE COUNT          733 a          392 c          470 g          703 t
ORIGIN      
        1 aagagatacg accaatgcct tatcagaaaa tgagcgttta accaataagc tcaaacaaac
       61 tgcgtcggct caaaaacatt tggcaaaagc gtataatttg aacgacaggt tatatcagac
      121 aggtattcaa agttatatag aaaccttaaa tagcaaaatt tcgctcgata aaatcaacat
      181 cagtttaaat caggataaat tacaagaact actaagcatt gtacgtttat atcaagaact
      241 ggctggaggt tatcgcgcca atttagagtc tgaaataaag tcttctgtct gagcaaacag
      301 aactctgtta taaattatta attacgaata ggggcgtatt tcatttttca tagtacccag
      361 cttatcttct tctacagtgc ctccctgatt cagtcttttg tggttgtttt tagtgcatag
      421 tgagcataaa tgggcatata tcaaggcagg atatgcaaat aaatattgca taatgtttaa
      481 ttttgtgaca ttatttgcgc cattgtgtat cctgtgagag ttgtgtatgt taaaaaaaac
      541 aatgtttttt attttgtcga tatctgcttc aagtattttt gctgctgaca atgtagattt
      601 gtatcaagcc cctctcaata gcatcaataa ataccctata ctacaaacac caaagaatgc
      661 gattatttta aagagttctt ctgctgttat tgataattca ttgcaaaaat taaatcaaac
      721 aaaagaagac aatcaaatga tagttcgtta tcaacaactg tataaaggaa tacctgttat
      781 tggcgcccaa gtgatgatta ctaaaggaac agattctgga gtgcagtcca atgataatgc
      841 agaggtgaac gggcatttat tggaaaatat agaacttaat acgaaaccgg ctattagtgc
      901 gcaacaagcg aaggaatatg caaaaaaatc ctattttcaa tttagctccc aatctaacat
      961 acaacaggaa acagctgaat tacagattcg gccagaccat aataaccaat taaagctggt
     1021 ttatttggtt tcatttaaaa gcgtgcagca ggatggtaag ccagactggc ctttttttgt
     1081 tattgatgct caaacaggag ctttgattaa gcaatggaac aatatcaaaa attatttgga
     1141 tacagggcct ggaggtaatg agaaagttca ggaatattgg tatggtaaag atggattacc
     1201 tgctttggat gtgactcaaa atggcagcca atgcgtcatg gaaaactcaa aagtcaagtt
     1261 ggttaatctc cattctcaat gggattggga aaacacgata aatactcctt ttgaatacgt
     1321 ttgtaacaat aatatagaag agaatattaa tggaggattt tctcctggca atgatgcgta
     1381 ttatttcgga catgttattg ttggtatgta caaagactgg tatggactta atgccttaca
     1441 acattctaat ggtgctccaa tgcaattggt tatgcgagtt cattttgggc aaaactatga
     1501 taatgctttt tgggatggac aagccatgtc atttggagat gggttggatt tttacccatt
     1561 ggtttcttta gatgtagccg gtcatgaagt gactcatggt tttacagagc agcattctgg
     1621 tcttgagtac catgatcaat caggtgcact taatgaatcc ctatctgata tggcaggaca
     1681 agcgtcaaga gcttatcttt tggaaaaaaa tcctcagttg tataacaaag cttacttaca
     1741 gcccaatgaa gtcacatggg gtattggtga aacaatagtt cgtgattctt atggcaaagc
     1801 tttgcgattc atggattacc catcctctga tggaagctct gcagattgtt tagacaaagg
     1861 tattgcgcaa aacaatggca gctattgtgc tatcagttat gatgaggtag tagcctatgc
     1921 caatacacat atcgcacttc ctcaagaacg ccagagcttc atagttcata cagccagcgg
     1981 tgtgttcaat aaggcttttt acctaatgtc taaggatatg ggtattaaaa acgcttatca
     2041 catcatggtt gttgctaaca caaaatattg gactcctacg acagacttta aaaatggagc
     2101 ttgcggagtc atttatgctg ccagggattt aaataccgat atcaataagg ttaagtccgc
     2161 ttttggtcaa gtaggtattg atacagccgg gtgtgttatt taggggagca aaagcagctc
     2221 ccggcaagtt tttgctccaa caaactgcgg tgatctcttg tggttcatcg cgagccattt
     2281 ttgctcgcag tgttgttt
//
LOCUS       DASLOE010000272         2287 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.273, whole genome shotgun sequence.
ACCESSION   DASLOE010000272 DASLOE010000000
VERSION     DASLOE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.273"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(18..1055)
                     /locus_tag="U6309_002905"
     CDS             complement(18..1055)
                     /locus_tag="U6309_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEN5506312.1"
                     /translation="MKLTRNMIAQAVQAKIITEEQAKKLIEFIKNLPEQTPGFNLTNV
                     LYYFGGLIAIGAMTLFMNLGWELFGGWGIFYLSVCYALLGLGLCSQFNGKGYVIPAGI
                     CATFVICLTPLAIYGLQKGMGWWPDDSVYQDYHVYIKWHWIFMELGTLMVGIILAWIY
                     RYPFMIMPIAVTLWYMSMDIAAMLGDGQFDFELRAMVSMYFGLITVLIAFWVDVRSSQ
                     SADYAFWLYIFGVMAFWFGLSCQSSDSEMSKFIYMCINLFMILTGVILNRKVFVIFGA
                     LGCSYYLGHLAFQVFKFSMLFPVALTAIGFGIIYLGIIWQRNEAKLTKELRSILPKPL
                     RDLLEARDSMD"
     gene            complement(1191..>2287)
                     /locus_tag="U6309_002906"
     CDS             complement(1191..>2287)
                     /locus_tag="U6309_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN5506313.1"
                     /translation="QWAFIAIWFQCIQNVLIYPTLLSFIAGTLLYSISPDLVQNKSML
                     FLIIIFLIWSLTWINLKGIHLSSRFNSYCSITGLILPFIIILFVGLYWWITQVKPGTT
                     VLPPAESYSWTSLTAIILSFCGIELAAVHARDSKQGAFPKAIAISVITIFLTMLFGSI
                     VLAMIIPPEQLNFISSIPQLIQLFFNKIGYGYFAFIINGLIAIGCIGAANNWLIAPIK
                     GLEFAINEGFLNRQLSQVNKHNAPFRLLVAQAFCVSIISALFLIVPSINASYWIMLNA
                     ATQMNLLMYLLLFISAIKIVSSKPILSKLNIMISAYLGLAGSGITLIVSLTPPPSLAI
                     SSKLIYALLGAIFLVIMTLIPIWRRPKVLT"
BASE COUNT          806 a          447 c          418 g          616 t
ORIGIN      
        1 cgggagaaaa tatttcatta atccatactg tcccttgctt ccaataagtc tcttaatggt
       61 ttgggaagta tggaccggag ctctttagta agtttcgctt catttctttg ccagattatt
      121 cctaaataaa tgataccaaa acctatggca gtcaaagcaa cagggaaaag catactgaat
      181 ttaaacacct ggaatgccag atgccccaga taataagaac atcctaacgc accaaaaata
      241 acgaatactt ttctgtttaa aatgactccg gtcagaatca tgaagaggtt aatgcacata
      301 tagataaatt tggacatttc actgtcggag gattggcaac ttaaaccgaa ccaaaaagcc
      361 atgacaccga atatatacag ccaaaaagca taatctgctg attgactgga tcgaacatca
      421 acccaaaagg caatcaatac agtaattaag ccaaagtaca ttgaaaccat tgcccttagc
      481 tcaaaatcaa attgaccgtc acccaacatg gctgctatat ccatggacat ataccaaaga
      541 gtgaccgcaa ttggcataat cataaaaggg taccgatata tccaggccag gataatacct
      601 accattagcg tacccaactc catgaagatc caatgccatt tgatataaac atgatagtcc
      661 tgataaacgc tgtcatctgg ccaccagccc attccttttt gtaagccata gattgccaaa
      721 ggagtaaggc aaatgacaaa agtagcgcaa atgccagcag gaatgacata tcctttccca
      781 ttgaattgac tgcaaaggcc taggccgagt agggcataac aaactgacaa atagaaaata
      841 ccccagcctc caaacagttc ccatccaaga ttcataaaca aagtcattgc accaatagct
      901 ataagtcctc caaaataata aaggacattg gttaggttaa atcctggagt ttgttcggga
      961 aggtttttta tgaattcaat gagtttcttg gcctgttctt ctgtaattat tttagcctgg
     1021 acagcctgag caatcatatt ccgtgtcaat ttcatcatca tctccgatga gtactgaatt
     1081 cctgctaaaa tttaacctga ttttacaata actaatgatt aatatctcaa ttatagctga
     1141 gagtacgaaa cttgcgtttg aatttgcaaa tcaggattgg atatttcttt tcatgtaaga
     1201 actttaggtc tcctccaaat aggaatcaag gtcattatga ccaggaaaat tgcaccaagc
     1261 aatgcataaa tcaatttgga gctaatggca agtgatggcg gtggtgtcaa acttacaatt
     1321 aaggtaatgc cactgcctgc caaccccagg taagcggaaa tcattatatt tagcttactc
     1381 aaaatcggtt ttgaggaaac tattttgata gcgctaatga agagcaagag gtacattaat
     1441 aaattcattt gtgtcgctgc attcaacatg atccagtaag atgcattaat cgaaggtacg
     1501 ataagaaata aagcactaat aatcgataca cagaaggctt gtgcgactaa caatctgaaa
     1561 ggtgcgttat gtttattcac ttgactgagt tgcctattta agaacccttc gtttattgca
     1621 aattccaatc cttttatagg tgcaatgagc cagttatttg ctgcacctat acaacctatt
     1681 gcgatcaatc cattgataat aaaggcaaaa tatccataac caattttgtt aaaaaataat
     1741 tgtatgagct ggggaatgct gctgataaaa tttaattgct ctggaggaat gatcatagcc
     1801 aatacgatcg aaccaaataa catcgtcaga aagattgtaa taactgaaat agctatggct
     1861 ttagggaaag ccccttgttt actatcccgt gcatgaaccg cggctaattc aataccgcaa
     1921 aatgacaaaa taattgcagt aagcgaagtc caggaataag attcggctgg tggtagaact
     1981 gtggtaccag gtttaacctg cgtaatccac cagtacaacc ccacgaacag gataataata
     2041 aaaggaagaa ttaatcccgt tatggagcaa taagaattaa aacgacttga gagatgaatt
     2101 ccttttaaat taatccaggt taaactccat atcaaaaaaa taataattaa aaaaagcata
     2161 cttttgtttt gaactaaatc aggggagatg ctgtagagca gggtgccagc aataaaactc
     2221 aataaggtag ggtatattaa aacattttga atacattgaa accaaatggc gatgaatgcc
     2281 cactgat
//
LOCUS       DASLOE010000273         2268 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.274, whole genome shotgun sequence.
ACCESSION   DASLOE010000273 DASLOE010000000
VERSION     DASLOE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.274"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1710
                     /locus_tag="U6309_002907"
     CDS             <1..1710
                     /locus_tag="U6309_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HEN5506314.1"
                     /translation="NYFTYMMFLAFVPAIPIIIMAFFLPYKRIIWIISVVAATTSLIF
                     LFVDSQIFSMFKFHLNKTILSFVFNSDWSVIFTFSRYELILSFFMVVFILFLESIVAW
                     LVWKKIVLKERLKIGKTISLFWLGGALISYFILLLSIAQNNNLFAQQIPNLPLYNQLI
                     AYTIPDKNADDILRRYSEHHFTQPLFPNYPLHYPLHPMQCKMPEKPYNIILIMVDSLR
                     YDSLRAKYMPNTAQFAKRSWQFSQHMSAGNATQPGLFSLFYSIPSNYSTAVLEQKKSP
                     VLIDLLLQHGYQTKIIWSGSMAPLPLDQTIYKKIANLNTNGAPIDDTGDKDRYSTREA
                     IQFLTDPKTNNPFFLHIFYNAPHDYCRYQSFPQLYKPAIEECVRIGMTNHDDPVPYYN
                     RYLNAVTFIDQEISKVLGVIEKKGYLKNSIIIITSDHGQEFNDNRQNYWGHTSNFTPI
                     QLHVPLIIHWPGELARRFNYLTSSYDIVPTIIQRLFACKNPVSDYSIGYNLLIEGNRA
                     SFLLVGSYINMGIFEADRATALETSGRITITGMKAEPLDDAVPRTDVITQALDLMRKY
                     YGK"
     gene            1838..2152
                     /locus_tag="U6309_002908"
     CDS             1838..2152
                     /locus_tag="U6309_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506315.1"
                     /translation="MANQTRENLINKAKNGSESIALIYSPIELHEYIVEIISKDSDHF
                     IHKGKEICHFNSIDEAVSRATELGAKEFFLCADNTYDECGSANSVQHFDYIPIYSKFK
                     RT"
BASE COUNT          715 a          395 c          372 g          786 t
ORIGIN      
        1 aattatttta cttatatgat gtttttagct tttgtccctg caataccaat cataataatg
       61 gcattttttt taccttataa acgaataatt tggattatca gtgttgttgc ggctaccaca
      121 agtcttattt ttttatttgt tgatagtcaa attttttcaa tgtttaagtt tcatcttaat
      181 aaaacaatat tatcgtttgt ttttaattca gattggagtg tcattttcac tttttccaga
      241 tatgaactga ttttgtcttt ttttatggtt gtctttatac tctttctgga gtctattgtt
      301 gcctggttgg tatggaaaaa aatagtttta aaagaacgtt tgaaaattgg taaaactatt
      361 tcattatttt ggcttggtgg ggcgttaata agttatttta tattgctctt atcgatagct
      421 caaaataata atttatttgc tcagcaaata cctaatttgc ccctttataa tcaactcatt
      481 gcttatacta tccctgataa aaacgcggat gatatattac gccgctacag tgagcaccat
      541 tttacacagc cacttttccc caattaccca ttgcattatc ccttacatcc catgcaatgt
      601 aagatgcctg aaaaacctta taacattatc cttataatgg tagattcact tcgctatgat
      661 tccctaaggg caaaatatat gccgaatacg gcacaatttg caaaaagaag ttggcaattt
      721 tctcagcata tgagtgctgg aaatgccaca caaccaggat tgttttcttt attttattcg
      781 attcccagta actactcgac ggcagtcctg gagcaaaaaa aatcaccagt tttaattgat
      841 ttattattac aacatggtta tcagacaaaa attatttggt ctggctctat ggctccacta
      901 ccattagatc agactattta taaaaaaatt gccaatctta atactaatgg agcgccaata
      961 gatgataccg gagacaagga cagatattcc accagagaag ctatccaatt tttaacagac
     1021 cctaaaacaa ataacccatt ctttctccat attttttata atgcgccaca tgactattgt
     1081 agatatcaaa gttttccgca gttatacaaa cccgcaatag aagaatgtgt acgaattgga
     1141 atgacaaatc atgatgatcc agtaccatac tacaatcgtt atttgaatgc agtcacattt
     1201 attgaccagg aaatttcgaa agtattaggg gttatcgaaa aaaaaggtta tttaaaaaac
     1261 agcatcatta taattacctc tgatcatggc caagaattta atgataatcg tcaaaattat
     1321 tggggacata ccagtaattt tacccctatt caactacacg ttcccttgat cattcattgg
     1381 ccaggggagt tagctcgtcg ttttaattat ctcaccagca gctatgatat tgtccctaca
     1441 ataatacagc gtttatttgc ttgtaaaaat cctgtttctg actacagcat cgggtataac
     1501 ttgctaattg aaggcaatcg agcctcattt ctattggtag gaagttatat taatatggga
     1561 atttttgaag cagatcgcgc cacagcatta gaaacctccg gaagaattac aattactggt
     1621 atgaaggcag agcctcttga cgatgcggta cccagaacgg atgttattac ccaggcattg
     1681 gatttgatgc gcaagtatta tgggaaataa agattgtctt taaaaataaa ttggcaatta
     1741 acctttttat tctcaattta ggtcatacag cggatatctt taaatttgta ggcaaaatat
     1801 ggctataatt atatataatt tgcttgggaa ttaagtaatg gcaaaccaaa cacgtgaaaa
     1861 tttaattaat aaggcaaaga acgggtccga atctattgct ttaatttatt ctccaataga
     1921 acttcatgaa tatattgttg aaattatttc taaagattca gatcatttta tccataaagg
     1981 aaaggaaata tgtcatttta attcgattga tgaagcggtg tctcgtgcta ccgaattagg
     2041 ggcaaaagag ttttttcttt gtgcagataa tacatatgat gaatgtggtt ctgcaaattc
     2101 tgtgcaacac tttgattata tacctattta ttctaaattt aagcgcactt gagtttggcg
     2161 catcaatctt ggttgcgaac tttttgcatt tctttttcgg ttcaaattct ataatccgat
     2221 gaataacaca ggctttgcct actttaaatc ttgtaggcac ctcttcgg
//
LOCUS       DASLOE010000274         2256 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.275, whole genome shotgun sequence.
ACCESSION   DASLOE010000274 DASLOE010000000
VERSION     DASLOE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.275"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1096
                     /locus_tag="U6309_002909"
     CDS             <1..1096
                     /locus_tag="U6309_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HEN5506316.1"
                     /translation="DLLLLMQFDTNLYNRCEAAKRLISVLINDFCIGKKIELSPQFFA
                     VYKALLSDNSLNEWMLAELITLPSLEELIENQDKPDFEKLNEGRQLIQNALANELKTD
                     FYNLLFRIQISGDDDKQKLKGFDLKQAGLRRLKSVCFSYLLNVDFEKTKEKLILQFED
                     ALGKNMTETALALSMLCEINCEEADVALEDYYHYWKNDPGAVNNWFSIQASAHSPDVI
                     ERVKKLMRHGDFDLSNPNKVYALLGSFIKNPFGFHSVTGEGYQLVADAILDLDRINPT
                     LAANLTEKFTYWDKYDVNRQAMMISTLKIIYSNATSSDVRTMAKKGLDKVKEDLPLPI
                     HLTFHGGSTMQDRTTQLIADGNKENAYQLH"
     gene            complement(1231..2154)
                     /locus_tag="U6309_002910"
     CDS             complement(1231..2154)
                     /locus_tag="U6309_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN5506317.1"
                     /translation="MSSKNTHSTLLNKQEINWILEQRITRLTDNKEVLIYPMGAERYL
                     SSALSGHEEERYNLHSEIKEKNVLIIPGYGNSAFLFAQAGAKSILVYDKDPVTIAWVK
                     AFKKYYHYREYNNNGKPYPSIGELLTALTKWYPPLLKLPSGVIKNSVFWIINPKLLRK
                     SYIYYMLKLTRQAVQLRVQNEFEFDHHIEFYAGELKHLIKNHPSKTFDTVFVPYLLGV
                     QNGIEKEKDIVSFIKQLIELVPYGHILVTPSKSTKEFHLVGQSYFCTTAYSNIQSIPD
                     LKPYVIAEDSIWFKTQGLAVFGIKPGAEKRL"
BASE COUNT          727 a          338 c          415 g          776 t
ORIGIN      
        1 ggatttatta ttgttgatgc aattcgacac taatttgtat aatcgttgtg aggcagcaaa
       61 acgacttatt tcagttttaa ttaacgattt ttgtatagga aaaaaaatag aattatctcc
      121 tcagttcttc gctgtttaca aagcattact ttctgacaac tctttaaatg aatggatgct
      181 agctgaatta attaccttac cgtctttaga agagttgatt gaaaatcagg ataagcctga
      241 ttttgaaaaa ttaaatgagg ggcgccaatt aatacaaaat gctcttgcaa atgaattgaa
      301 aacggatttt tataatctcc tttttagaat acaaatatct ggggatgatg ataaacaaaa
      361 attaaaaggt tttgatttga agcaagcggg attgcggcgt ttaaaatccg tatgtttttc
      421 ttatttgcta aatgttgatt ttgaaaaaac aaaggagaaa ctgattcttc aatttgagga
      481 tgctttaggc aaaaatatga cagagactgc tttggcttta agcatgttat gcgaaataaa
      541 ttgtgaggaa gcggatgttg cattagagga ttattatcat tattggaaaa atgatccggg
      601 agccgtaaat aactggttca gtattcaagc atcggcgcac tcccctgatg tgatagagcg
      661 ggtaaaaaaa ttaatgcgtc atggtgattt tgatttgtct aatcctaaca aggtgtatgc
      721 tctgctagga tcatttataa aaaacccatt tggatttcat tctgttacag gtgaaggtta
      781 ccaactggtt gctgatgcca ttttggactt ggatagaatt aatccaactt tggctgccaa
      841 tctaactgaa aaatttactt attgggataa atacgatgta aataggcaag ctatgatgat
      901 tagcacttta aaaataatct actctaatgc tacttcatct gatgtgagaa caatggcaaa
      961 aaagggattg gataaggtaa aagaagatct cccattacca atccatttga cttttcatgg
     1021 gggatcaacc atgcaggata gaacaactca attaattgct gatggaaata aggaaaatgc
     1081 ttatcaattg cattgatttc ttatttcaga acctgatttg gaaaagcgcg agatattgat
     1141 attgcctaaa acttgggggg tgaatactcc ccttccttga taggtaatga ggaagcacgt
     1201 tttctaatac aataaatcat agtcttattt tcacaaacgt ttctctgcac ctggttttat
     1261 accaaatact gcaagtcctt gagttttaaa ccaaatactg tcttctgcaa tgacataagg
     1321 ttttaaatca ggaatggact gtatattaga ataggctgtt gtacaaaagt aactttgccc
     1381 aaccaggtgg aattctttag tgctttttga aggggtcact aaaatatgac catagggtac
     1441 aagctctatt aattgcttaa taaaactaac aatatctttc tctttttcta ttccattttg
     1501 tacacctaag aggtaaggaa caaatactgt atcaaatgtt tttgacggat gattttttat
     1561 cagatgtttt aattcacctg cataaaactc tatatgatga tcgaactcaa attcattttg
     1621 aactcttaat tgtacagctt gcctggtgag tttcagcata taataaatat atgattttct
     1681 tagtaattta gggttaatta tccaaaatac agagttttta atcactcctg aaggtagttt
     1741 aagtagagga ggataccatt tggtgagagc tgtcagaagt tcacctattg aaggatatgg
     1801 ttttccatta ttattgtatt ctctgtaatg gtaatatttt ttaaatgctt ttacccatgc
     1861 aatagtcacg ggatctttgt cataaacaag aatagatttg gcccctgctt gagcaaataa
     1921 aaaagcgcta ttaccatatc cagggataat taggacattt ttttctttga tttcactgtg
     1981 taaattatac ctttcttctt catgaccaga taaagcagat gacaaatagc gttctgctcc
     2041 cattggataa attaaaactt ctttattgtc agtcagtctt gtaatacgct gctctaaaat
     2101 ccagtttatt tcttgcttat tgagtaaagt gctatgggtg tttttacttg acatgaatag
     2161 gcctttcata aaaggattag aactggctgg tgctaaatgt gagtattaat ttagtcgaca
     2221 tttaattata ttatactatg ttattgattt aaattt
//
LOCUS       DASLOE010000275         2239 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.276, whole genome shotgun sequence.
ACCESSION   DASLOE010000275 DASLOE010000000
VERSION     DASLOE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.276"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..594)
                     /locus_tag="U6309_002911"
     CDS             complement(<1..594)
                     /locus_tag="U6309_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEN5506318.1"
                     /translation="MFLQTSQKLPKGMFKACVVPRPIAWIGTKDSEGNHNLSPFSFFN
                     IVCEEPPMVMFATTGAHNSGGAKDTLKNVEDTKEFTVNLVSYGSKEAMNITSLDFSRG
                     ISEFDMAEVDHLPSRLIQTPRVKESPISLECVYHESIQLPIPIHSNLINRMVIGKVVG
                     IHLKPEILTKDKRIDTNKLNLIARVGYNSYTKVKAHFD"
     gene            763..2109
                     /locus_tag="U6309_002912"
     CDS             763..2109
                     /locus_tag="U6309_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506319.1"
                     /translation="MFTTIDNNEGIVQTYWTEVRNKFEKADPYLSKLIDNVSPDKLPI
                     YLLYFPYGMLKGDTKSSYMPLLDGGYIKLSDTGVDKKIVNDLGYGMYSSPLGMVLDKF
                     IEYFIEFDDKIFTYYISGPGTIFNTGMLLKNKNSRNYSPNGVLKATAGARTAFMLPSI
                     NSHNGINKLSKLVNQDLTTPRNHNDHFELFKAINQHDNSNWKVCLAYFSEKWVKHLLT
                     DPAWVEIKNYMLEAKNKNDSFSVNSAYYDIFYSKAQKDRNLRTSSPYLTNTAIHLIKI
                     ALGEHPGYVPATTANFLPIESIQKFISESFQLKRTPTIMVPHSLVYEKEKEPVYYSLQ
                     NPTTPHFLTKKNEKVTANQEIDIIYRILNKFIEEMSKQDSLLAGTVFSDISDHIRFNY
                     FHNYPPKDSNLINNSRQLSKLDPRFNFSSYKNGESEFCFEGQFLRGCIQIQPNVKE"
BASE COUNT          764 a          377 c          368 g          730 t
ORIGIN      
        1 atcaaaatga gctttgactt tggtatatga gttatatccg actcttgcaa tgagattaag
       61 tttattggta tcaatgcgtt tatctttggt taaaatttcc ggtttaaggt gaattccaac
      121 aaccttaccg attaccattc ggttaattaa attagaatga attggtattg gtaattgaat
      181 tgattcatga taaacacact ctagactgat aggtgattct tttacccgag gtgtttgaat
      241 tagcctacta ggtaaatgat ctacctcagc catatcaaat tcgctaattc ctcgcgaaaa
      301 atccagggaa gtaatattca ttgcctcttt acttccatag ctgaccaagt taactgtaaa
      361 ttctttagta tcttcaacgt tttttagagt atctttggca ccgccgctgt tatgagctcc
      421 tgtcgtagca aacataacca ttgggggttc ttcacataca atgttaaaaa aactaaaagg
      481 cgataaatta tgattgcctt cagaatcctt ggtgccaatc caagcaatag gccttggaac
      541 aacacaagct ttaaacattc cttttggtaa tttctgcgat gtctgtagaa acatggtgat
      601 atattccctt atttaaattt ccccaacgct gtttttaagt tgggatgctt gacgctacag
      661 attcaatcat gattgacttt ttagtaaaac tatttttaaa atggataata ttaagtgtta
      721 taaaaaaccc tttttataat gggtttttaa caaggatgtt tgatgtttac cacaattgat
      781 aataacgaag gtatagtcca aacatattgg acagaagtaa gaaataagtt tgaaaaagca
      841 gatccctacc tttctaaact tatagacaat gtctctcctg ataagttacc aatttactta
      901 ctttattttc catatggcat gttaaaaggt gatacaaaaa gctcttacat gcccttgctc
      961 gatggaggtt atattaaatt atctgataca ggagttgata aaaaaatagt caatgactta
     1021 gggtatggaa tgtactcatc cccattaggc atggtacttg ataagtttat tgaatatttt
     1081 attgaatttg atgacaaaat ttttacttat tatatctctg ggcctggtac catatttaat
     1141 acagggatgc ttttgaaaaa taaaaattca agaaattata gtccaaatgg cgtactaaaa
     1201 gcgactgcgg gtgcacgaac agcttttatg cttcccagca taaacagtca caatggaatc
     1261 aataagcttt ctaaacttgt caatcaagat ctaacaaccc cgagaaacca taacgatcac
     1321 tttgaactat ttaaagctat aaaccagcat gataatagta attggaaagt ttgccttgcc
     1381 tacttctcag aaaaatgggt gaaacatttg cttactgatc ctgcttgggt tgaaattaaa
     1441 aattatatgc tagaagctaa aaataagaat gattctttta gtgtgaatag tgcatattac
     1501 gatatttttt attctaaagc ccaaaaggat agaaatcttc gtacatctag cccatattta
     1561 acaaacacag ctatacattt gatcaaaatc gctcttggtg aacatcctgg gtatgtaccg
     1621 gctacaactg caaatttcct acctatagaa agcatacaga aatttatttc tgaatccttc
     1681 caattgaaaa ggactcccac aataatggtt ccacactcac tagtgtacga gaaagaaaaa
     1741 gaaccggtat attattcatt acaaaaccca acgacaccgc attttcttac taagaaaaat
     1801 gaaaaagtta ctgcaaatca agaaattgat attatttata gaattttaaa taaattcatt
     1861 gaagaaatgt ctaagcaaga ctctctactt gcaggtactg tattttctga tatttcagat
     1921 catattagat ttaattattt tcataattac cctccgaagg atagcaatct gataaataat
     1981 tctcgccagc tttcaaaatt agatccaaga ttcaatttta gtagttacaa aaatggggag
     2041 tctgagtttt gttttgaagg tcaattttta cgaggctgta tacaaatcca acccaatgtt
     2101 aaggaataag atcaggtttt atatatgctg tggccaacct aggtatattc actttatcta
     2161 aatttgaaag gcaaatgtca aatttctcac aaggaatttg gggatatata gatgcaacat
     2221 agagaacaat aggatgcgt
//
LOCUS       DASLOE010000276         2198 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.277, whole genome shotgun sequence.
ACCESSION   DASLOE010000276 DASLOE010000000
VERSION     DASLOE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.277"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..573
                     /locus_tag="U6309_002913"
     CDS             <1..573
                     /locus_tag="U6309_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEN5506320.1"
                     /translation="IPVLMALIGIWNNNFLNIHNLLILTYSKKLEYFVPYVQQLDMES
                     NGKSIDVNGRMVDYATGPIVWGGLGNQAQHSYFQLLCQGTHRCVGDFITLKTNDEHEI
                     NSMCHYKMKVLSEGIQTIENPYGYIPGNMPMNHLILSDCSPYTLGALVALYEHKIFVQ
                     SVIWNINPFDQPGIESAKSAHREITLSSES"
     gene            728..1603
                     /gene="rfbA"
                     /locus_tag="U6309_002914"
     CDS             728..1603
                     /gene="rfbA"
                     /locus_tag="U6309_002914"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEN5506321.1"
                     /translation="MKGIILAGGFGTRLYPLTKSISKHMIPVYDKPMIYYAISTLMLA
                     NIREILIISTEEHLPLYQNLLSDGSQWGISFAYLVQNEPRGIAEAFILGEKFIGNDSV
                     CLVLGDNIHQGRGFSELLQNAKSKLNGATVFAYYVDKPQAYGVVEFNEKQQVLSIVEK
                     PAQPKSNYAVTGLYFYDNQVIEIAKSLKPSGRGELEITDINQFYLYENKLDVQILGRG
                     FVWLDMGTPETLLSASNYICTIEQRQGLKIGCPEEIAWRMKFISDDELLCRARELNKS
                     SYGQYLAELLNHKRY"
     gene            1624..>2198
                     /locus_tag="U6309_002915"
     CDS             1624..>2198
                     /locus_tag="U6309_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN5506322.1"
                     /translation="MAKILITGATGFIGRSLVPALLSEGHDVRCAVLQLDSTLQAEQI
                     VINNLEVHTDWTDALWNVEIVIHLAARVHIMKEYAISSLDEYCKINSIATKNFAEQAA
                     QNNVKRFIFLSTIKVHGEFSQNSLPVTEDCRTQPEDPYAKSKLYAEQFIQEICQNTRM
                     EFVILRPPLVYGPYVKANFLRILQLVDKKWP"
BASE COUNT          713 a          338 c          452 g          695 t
ORIGIN      
        1 attcctgtgc taatggcttt aatcggtata tggaataaca attttttgaa tattcataat
       61 ttacttattc ttacctattc aaaaaaactg gagtactttg ttccttatgt acaacagctg
      121 gatatggaga gtaatgggaa atccattgat gtgaatggaa gaatggtaga ttacgccact
      181 ggacctatcg tttggggtgg attaggaaat caggcacagc atagctattt tcaattatta
      241 tgtcagggta cgcatcgatg tgttggcgat tttattactt tgaaaactaa tgatgagcat
      301 gagattaatt ccatgtgtca ttataaaatg aaagttttaa gtgagggaat tcaaacaatt
      361 gaaaatcctt atgggtatat tcctggcaat atgccaatga atcatctcat tttgtcagat
      421 tgttctcctt atacattagg ggctctggtg gcactctatg aacataagat ttttgtacaa
      481 agtgtgatat ggaatattaa cccattcgat cagcccggta ttgaaagcgc caagagtgct
      541 cacagagaaa tcacattgtc aagcgaatca taaaggggcg ctcaatgcta taaattagtt
      601 taattgttaa actaaacttt atattttatt gacattatca acgtatttta atgtattact
      661 tcaataaact tgataatatt taaggctgtt atttgttcaa agaaaaatag agagtaggct
      721 cgatataatg aaaggtatta tcctggcagg tggttttggt acaagactgt atcctctgac
      781 aaaatccatt agtaagcata tgattccggt ttatgataaa ccaatgattt attatgccat
      841 ctctacatta atgttggcta atatcaggga aatactgatt atttcaacgg aggagcatct
      901 tccgctgtat caaaatttat tgagtgatgg ttctcagtgg ggcatctcat ttgcctatct
      961 tgtgcaaaat gaaccaaggg gaatcgcgga agcattcatt ttaggtgaaa aatttattgg
     1021 caatgattct gtttgcctgg ttttggggga taatatccac cagggcagag gtttttctga
     1081 attattacaa aatgcaaaat caaaattaaa cggtgcaaca gtttttgcct attatgtcga
     1141 taaacctcag gcctatggtg tggtggagtt caatgaaaaa caacaagtcc tatccatagt
     1201 ggaaaaacca gcacagccca aatccaacta cgcagtaact ggattatatt tttatgataa
     1261 tcaggttata gaaattgcca agagtttaaa accctctgga aggggggagc tggaaataac
     1321 tgacatcaat cagttttatt tatatgaaaa caaactggat gtccagattt tgggaagagg
     1381 ttttgtctgg ctggatatgg gtacaccaga gaccctgtta tcagcaagta actatatctg
     1441 tactatcgaa cagcggcaag gtttaaaaat tggatgccct gaagaaatcg cgtggcgtat
     1501 gaagtttatt agtgatgatg agttactgtg cagggctaga gagcttaata aaagtagtta
     1561 cggtcaatat cttgcagaac tgcttaatca taaacgatat taattttttc aggttataaa
     1621 gtagtggcaa aaatattaat tactggcgcg acaggattta taggacgaag tttagttcct
     1681 gccttgcttt ctgaaggaca tgatgtgcgt tgtgctgttt tgcagctgga tagtacatta
     1741 caagcagaac aaatagtaat aaacaatctt gaggttcaca ctgattggac tgatgctttg
     1801 tggaatgtgg aaatagtcat tcatttagct gccagagttc acataatgaa agaatatgca
     1861 atatcttctt tagacgaata ttgcaaaatt aatagtattg ctacaaaaaa ttttgctgag
     1921 caggcagctc aaaataatgt aaagcgattt atatttctta gtaccataaa agtacatggc
     1981 gaattttcac aaaatagtct gccagttact gaagattgta ggactcaacc tgaagatccc
     2041 tatgcaaaaa gtaagctgta tgctgaacaa tttatacaag aaatttgcca gaatacccgg
     2101 atggagtttg ttatattaag accaccgttg gtctatggac cttacgtgaa ggctaatttt
     2161 ctcaggatat tacaacttgt tgataaaaaa tggccttt
//
LOCUS       DASLOE010000277         2177 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.278, whole genome shotgun sequence.
ACCESSION   DASLOE010000277 DASLOE010000000
VERSION     DASLOE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.278"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            3..746
                     /locus_tag="U6309_002916"
     CDS             3..746
                     /locus_tag="U6309_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN5506323.1"
                     /translation="MLIEHQIKHYRTLYEWFQSPLGVFVAKEFTDQLEPVKKFLKGET
                     LLQLGDCATNPWLNLLDFKHKWIASPFSSTNRIHLECSLNQLPLHRDTMDCVIVPLTL
                     EPFGNNFSLIDEIDRILKPMGFLIFLCINPWSLWGGAMKWGLLNCYGNRTVKLRSAFN
                     LNRIFLQRGYKQCSLTNFCYIPPVNSQSLIKKLTFFDEIGKMIWPFPSGFYCYITQKY
                     EYISPSLIVEPAIESVKKEYDSPLQPATN"
     gene            929..1255
                     /locus_tag="U6309_002917"
     CDS             929..1255
                     /locus_tag="U6309_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HEN5506324.1"
                     /translation="MTKKSLFKISFANQDEIYEIYARSIKESDMFGFLEVEELVFGEQ
                     TALVVDPSEEKLKMEFCDVKRIFIPVHSIFRIDEVSKQGTSKVKDNLSHGNKVRPFPG
                     AGKIKD"
     gene            1298..1867
                     /locus_tag="U6309_002918"
     CDS             1298..1867
                     /locus_tag="U6309_002918"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEN5506325.1"
                     /translation="MTIPDKIKAVYEKSTCLYTSNEVEAALDRMAIKIHETLQDKNPV
                     IICVMVGGLVPLGNLLHRLDFPLEVDYVHATRYRGDLTGGDILWKVRPSSNLAGRTVL
                     VVDDILDGGITLAAIINEIKAMGAAEVYSAVLVDKYRKRVPNGLQKADFVGLQVEDHY
                     IFGYGMDYNEYLRNAPGIFIVHPDHEANK"
     gene            complement(1869..>2177)
                     /locus_tag="U6309_002919"
     CDS             complement(1869..>2177)
                     /locus_tag="U6309_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEN5506326.1"
                     /translation="GIQISIDDFGTGYSCLSYLKDLPINVLKIDQIFVKDLKNNQSNQ
                     TLIKAIIAMAKNFNLSLIAEGIQNKHELVFLQNLGCHYGQGYYFYHPMPPEKCTQFLQ
                     "
BASE COUNT          723 a          347 c          405 g          702 t
ORIGIN      
        1 ttttgttgat tgaacatcaa ataaaacatt atcgcacttt atacgaatgg ttccaatcac
       61 ctttaggagt atttgttgct aaagaattta cagatcaatt agaaccagta aaaaagttct
      121 taaaaggaga gacgttacta caacttggag attgtgctac aaatccctgg ttaaaccttc
      181 ttgattttaa acacaaatgg atagcatcac cattttcttc tacaaataga atccatcttg
      241 agtgttcgct aaatcagttg ccactgcata gagatactat ggattgtgtt atagtaccct
      301 taacactaga gccgtttgga aacaatttta gcttaattga tgaaattgac cgaatattga
      361 agcctatggg atttctcata ttcttatgca ttaacccttg gagtttatgg ggaggggcta
      421 tgaaatgggg gttacttaat tgttatggta atcgaacggt aaaattgcgt tcagctttca
      481 atcttaacag aattttcctt caaagaggct acaaacagtg ttctttaact aatttttgct
      541 atataccacc agtaaatagc caatctttaa ttaaaaagtt aacttttttt gatgagattg
      601 gcaaaatgat atggcctttt ccttctggtt tttattgcta tatcactcaa aaatatgagt
      661 acataagccc atcactcata gtcgaacctg ccatagaatc agtaaaaaaa gagtatgact
      721 cgcccttaca gccagcaaca aattagacgg aatatgtgat tcattaataa cctacatacg
      781 aaagaggtta aattattatc accattagac ctaaatgagt aaatttagtg ctaataatga
      841 gctgtatacg actaaactac tctaaatatt tgaagataca cccttacttt ttgtatactt
      901 tatcctgctt ttagataagg ttagatacat gacaaaaaaa tcgctattca aaatcagttt
      961 tgctaatcag gacgaaattt atgaaatcta tgctcgttca ataaaagaaa gcgatatgtt
     1021 tggttttctt gaggttgagg agctggtttt tggagagcaa acggcactgg tagttgatcc
     1081 gtctgaagaa aaacttaaaa tggaattttg tgatgttaag cgcattttta tccctgtaca
     1141 ttccatattt cgcattgatg aagtttcaaa acaagggaca tcaaaagtca aagataactt
     1201 gagccatggc aataaagtaa ggcctttccc tggagcaggg aagataaaag attaacttcg
     1261 tttagcctgt ataaacaact tctaaggaaa taaataaatg accattcctg ataaaattaa
     1321 agctgtatat gaaaaatcaa cctgtttata cacatctaat gaagttgaag cagcactgga
     1381 caggatggca ataaaaattc atgaaacact acaggataaa aaccctgtaa ttatttgcgt
     1441 gatggtagga ggtctagttc cattaggtaa tttattgcat cgtttggatt ttcctctaga
     1501 ggtggattat gttcatgcta cgcgttaccg aggtgattta acgggagggg acattctttg
     1561 gaaagtgaga ccatcaagca atctcgcagg acgaacagtc cttgtcgtag atgacattct
     1621 tgacggtggt ataaccttgg ctgccataat taatgaaatc aaagccatgg gtgctgcgga
     1681 agtttatagt gctgtattag ttgataaata tcgtaaacgc gtccccaatg gtttgcaaaa
     1741 ggctgatttt gtcggtttac aagtagagga tcattatatt tttggctatg gtatggatta
     1801 caacgaatac ttacgtaatg cgccagggat ttttatcgtt catccggatc atgaggcaaa
     1861 taaataaatt attgtaaaaa ttgtgtacat ttttcaggtg gcatgggatg ataaaaatag
     1921 tatccttgac cgtaatgaca acctagattt tgtaaaaaga caagttcgtg tttgttttga
     1981 attccctctg caatcaaact caagttaaaa ttttttgcca tcgcaattat agcttttatt
     2041 agagtttggt tggattgatt attttttaga tccttaacaa agatttgatc aatttttaaa
     2101 acattaattg gtaagtcttt taagtaactt aagcaagaat aaccagtacc aaagtcatca
     2161 attgaaattt ggatacc
//
LOCUS       DASLOE010000278         2176 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.279, whole genome shotgun sequence.
ACCESSION   DASLOE010000278 DASLOE010000000
VERSION     DASLOE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.279"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            220..558
                     /gene="rpsF"
                     /locus_tag="U6309_002920"
     CDS             220..558
                     /gene="rpsF"
                     /locus_tag="U6309_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEN5506327.1"
                     /translation="MRHYEIMFLVHPDQSEQVPGMVERYEGIITKHNGKIHRKEDLGR
                     RQLAYSINKVHKAHYILMNVECNLDALNEIKNAFKFNDAILRHLITVQKQAITTESVL
                     MKKEKETKVA"
     gene            572..799
                     /locus_tag="U6309_002921"
     CDS             572..799
                     /locus_tag="U6309_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEN5506328.1"
                     /translation="MSAYFRRKKMCRFSAEGGNEIDYKDINLLKNYITETGKIVPSRI
                     TGTQTRFQRQLAKAIKHARFIGLLPYCDSHR"
     gene            801..1712
                     /locus_tag="U6309_002922"
     CDS             801..1712
                     /locus_tag="U6309_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506329.1"
                     /translation="MNLAGNFILRQSRATLESKRYAIVCAVVLSIVPFASWLSVALMS
                     LITLRKGEKPGFEVMLPAMIVHSVPLMMFLPLEGALINTLVAYLPCYIAALTLRKTAS
                     WSNVFGAFLIQAFLVFLSIEILIPDFVSDQFNHFKVLLSQFQDYKQLVESSTDGLNNL
                     SLAQLFFGIQILSVIASALISLVFARTIQAKLFMPGGFKQEILEFRSGRFSFLMLIIV
                     SVASYFEVPVAISTLPLLLAYFFLSGFSLAYYILARKWQVRVVVLLFLLILLKPFFVL
                     FAYIVFGSLDSLFNFRLYLPARVREST"
     gene            1725..2174
                     /gene="rplI"
                     /locus_tag="U6309_002923"
     CDS             1725..2174
                     /gene="rplI"
                     /locus_tag="U6309_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEN5506330.1"
                     /translation="MEVILLEKVRNLGNLGDKVHVKSGYGRNYLIPQNKAVFATEQNI
                     ELFEKRRAELEKKAQQALANAEQRAAKLNDTTIVISAMASDEGKLYGSVGVNEIKDAL
                     IEKQIEISKREIVMPEGPLHSIGNYVVEVHVHSDVVANLQVEIIPAK"
BASE COUNT          705 a          333 c          413 g          725 t
ORIGIN      
        1 aattgctgta aaatttataa aaaaatttat ttaaagcaac tatgtaattt tattgcctat
       61 aaaataggca aagttatgat acgcagctct aataaatatc ttattttaaa tcgcaatgag
      121 attgtaagga ctaaataaaa aagttataat tggcagcttt ttaaaaccct tgccttacca
      181 gtactcaatg ctggaaggct ttatccataa ggagaattca tgagacatta tgaaattatg
      241 tttctagtac atccagatca aagcgaacaa gtacctggta tggttgagcg ctatgaaggg
      301 attattacca aacataacgg caaaatacat cgtaaagaag atttaggtcg acgccagctg
      361 gcatattcaa taaataaggt tcataaagca cattatatct taatgaacgt agaatgtaat
      421 cttgacgcac ttaatgaaat caagaatgca ttcaagttta atgatgctat tttaagacac
      481 ttaatcactg ttcaaaaaca agcgattact acagaatctg tcttaatgaa gaaagaaaaa
      541 gaaactaaag tcgcttaaga ggagaacatc tatgtcagct tattttcgta gaaaaaaaat
      601 gtgccgcttt agtgctgaag gtggtaatga aatagattat aaagacatca atctattaaa
      661 aaattatatt actgaaactg gtaaaattgt accaagccgt attactggta ctcaaactcg
      721 tttccaaaga caattagcca aggcaattaa acatgccaga tttattggtt tattgcctta
      781 ttgcgacagc catagataaa atgaatcttg caggtaattt tatactcaga caaagtagag
      841 caacgcttga aagcaaacga tatgctattg tttgtgcggt tgttctttca atagtcccat
      901 tcgcctcttg gctatcggtg gctttaatga gtttaattac tttgaggaaa ggtgaaaaac
      961 ctggatttga agtcatgtta cctgcgatga ttgttcattc tgtgccttta atgatgtttt
     1021 tacctctgga aggggcattg atcaatacat tggttgctta tttgccttgc tatatcgctg
     1081 ctttgacttt acgcaaaaca gccagctggt caaatgtatt tggagctttt ctaattcaag
     1141 cattccttgt atttctgtcc attgaaatat tgatacctga cttcgtaagt gatcaattta
     1201 atcattttaa agtgctattg tctcaatttc aggattacaa acagttggta gaaagcagta
     1261 cagatggatt gaataattta agtttggctc agttattttt tggtatccag atattaagtg
     1321 tgattgcttc agccttaatc tcactagtgt ttgcccgaac tatacaagct aagcttttca
     1381 tgcccggtgg ttttaaacag gaaatattag aatttcgtag tggcagattt tcatttttga
     1441 tgttaattat tgtgtctgtt gcctcttatt ttgaggtacc agtagctatt agcactttac
     1501 cactacttct tgcatatttc tttttatcgg gtttttcttt ggcctattat attttagcca
     1561 gaaagtggca agttagagtg gtggttctgt tatttctatt gatcttgtta aaaccattct
     1621 ttgtcttatt tgcctatatt gtatttggct cgctagatag tttatttaat tttcgattat
     1681 atctacctgc aagggtgaga gaatcaacgt aaggggttat taagatggaa gtaatcttat
     1741 tagaaaaagt acgtaatctg ggaaatctag gtgataaagt acacgttaaa tcaggttatg
     1801 gacgcaatta tttgatacca caaaataagg ccgtctttgc aacagaacaa aatattgaat
     1861 tatttgaaaa acgtcgtgct gaactggaga aaaaagcgca acaggctttg gctaatgcgg
     1921 aacaacgtgc tgctaaattg aacgatacca cgatagttat tagtgctatg gctagtgacg
     1981 aaggtaaatt gtatggttcc gttggggtta atgaaattaa agacgcattg atagaaaagc
     2041 aaattgaaat cagcaagcgt gaaattgtta tgcctgaagg ccctctacat tctataggta
     2101 attatgttgt tgaagtacat gtacatagtg atgtagtagc taaccttcaa gttgaaatta
     2161 tccctgctaa ataatt
//
LOCUS       DASLOE010000279         2172 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.280, whole genome shotgun sequence.
ACCESSION   DASLOE010000279 DASLOE010000000
VERSION     DASLOE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.280"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            113..1978
                     /gene="rpoD"
                     /locus_tag="U6309_002924"
     CDS             113..1978
                     /gene="rpoD"
                     /locus_tag="U6309_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEN5506331.1"
                     /translation="MTMNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHF
                     DVIISMLEGMNIKVFELPPSEDELALLLNTEEVPDIEEAAMVLASVDKETGRTTDPVR
                     MYMREMGTVELLTREGEIRIAKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIR
                     LNEIISGFADVDEEIAPATNIGSMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPE
                     QAKIYFDELREAFEKAMKALKEHGRTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHF
                     RQLRNHIREFERSIMRLCIEKARIPRKLFIDTFPGQETDLSWLETIISKHAKKLDLDR
                     IEENREEILRLQSKLASFEIEYGLTISEIKDINRKMSIGEAKARRAKKEMVEANLRLV
                     ISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIAD
                     QARTIRIPVHMIETINKLNRISRQILQETGREATPEELAEKMELSEDKIRKVLKIAKE
                     PISMETPVGDDDDSHLGDFIEDNNIESPIDMATAEGLREATLEILETLTPREAKVLRM
                     RFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEKLRSFLEGDEG"
     gene            2068..2144
                     /locus_tag="U6309_002925"
     tRNA            2068..2144
                     /locus_tag="U6309_002925"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2102..2104,aa:Ile,seq:cat)
BASE COUNT          724 a          432 c          470 g          546 t
ORIGIN      
        1 atgggcaagc tgaaattgaa aaataaaaat actgaaatac tggtatgttg cagccaccca
       61 gtttataatt taaaactttt tgtaatttcc cattgttcca acagaagtgc ctatgaccat
      121 gaatgaccaa gatcagcaaa gctcacaaat tacgaaagtc attagcctgg gtaaagagca
      181 aggttatttg acttacagtc aaattaatga cttattacct aatattgtgg atacagagca
      241 ttttgatgta atcattagca tgctggaagg catgaacatc aaagtttttg agcttccacc
      301 cagtgaagat gaacttgcgc tcttattaaa tactgaggaa gtcccagata ttgaagaagc
      361 agcgatggta ctcgcctctg tggataaaga aacaggcaga acaactgacc cggttcgtat
      421 gtatatgcgc gaaatgggca cagtagaact gctgactcgt gaaggtgaaa tccgcattgc
      481 caaacgcatt gaagaaggaa tttaccaggt tctaaaatcc ctggcgcatt atccggaaac
      541 agtgcaactg gttcttgaag actatgatcg tttccttgcg gaagaaattc gcctcaatga
      601 aatcattagt ggttttgcgg atgttgatga agaaatagca cctgctacta acattggttc
      661 catgctggat gaagagcaac aggaagaaat acttcttgat gtcgatgagg aagatgaaga
      721 cggcgaaggt ggtatatctg atgtggatga tggacccaat cctgagcaag caaaaattta
      781 ctttgatgag ctgcgtgagg cctttgaaaa ggcaatgaaa gccctcaaag agcatggccg
      841 cacgaataaa aaaacggttg agttgctcga ttccatgtcg gaatcttttt tgaaactcaa
      901 attgacttcc agacaagttg ataaactaac tcgtcatttt cgccaattgc gaaatcatat
      961 ccgtgaattt gagagaagca taatgcgtct ttgtatcgaa aaagcacgca tccccagaaa
     1021 attgtttata gataccttcc caggccagga aacagatcta agctggttag aaaccattat
     1081 cagcaaacat gccaaaaaac ttgatcttga tcgtatagaa gaaaatcggg aagaaatact
     1141 acgcttacaa tccaagctgg cctcttttga aatcgaatat ggtttaacca ttagtgaaat
     1201 caaagacatt aaccgcaaga tgtctattgg cgaagccaaa gcaaggcgtg ctaaaaaaga
     1261 aatggtcgag gctaacttgc gtctcgtgat ttccatcgcc aaaaaataca ccaaccgcgg
     1321 cttgcagttt cttgatttga tccaggaagg caacattggc ttgatgaaag cagtcgataa
     1381 gttcgaatac cgccgtggtt ataagttttc tacctatgcg acctggtgga ttagacaagc
     1441 gattacacga tccatagccg atcaggcgcg gactattcgt attccggttc atatgattga
     1501 aaccatcaat aagctcaacc gtatttctcg ccaaattttg caagaaactg gccgggaagc
     1561 gacaccggaa gaattggccg aaaaaatgga gttaagtgaa gataaaattc gcaaggttct
     1621 caagatagcc aaagaaccta tttccatgga aacccctgtc ggcgatgacg acgactctca
     1681 tctgggtgat tttattgaag acaacaacat tgaatcacca atcgatatgg caacagctga
     1741 aggtttaagg gaagccacat tagaaatact ggaaaccctg accccacgag aagccaaggt
     1801 tctgcgtatg cgttttggta tcgaaatgaa tacagaccat actctggaag aagttggtaa
     1861 acaatttgac gtaacccgcg agcgtattcg ccaaatcgaa gcaaaagcac tacgcaaatt
     1921 gcgccaccct tcacgttcag aaaaactaag aagcttcctt gaaggtgacg aaggttaatt
     1981 taatgactgt ttttataaac agtcattaaa aaaatataaa aaatagtaga acattaaaga
     2041 aattaagttt aaaatgaccc cctctccggg cccatagctc agttggtcag agcagcggac
     2101 tcataatccg ttggtcctag gttcaagtcc tagtgggccc accaataaaa tcaagtagtt
     2161 acgagtagtt ta
//
LOCUS       DASLOE010000280         2167 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.281, whole genome shotgun sequence.
ACCESSION   DASLOE010000280 DASLOE010000000
VERSION     DASLOE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.281"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            133..654
                     /gene="aroK"
                     /locus_tag="U6309_002926"
     CDS             133..654
                     /gene="aroK"
                     /locus_tag="U6309_002926"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HEN5506332.1"
                     /translation="MVKVRNIFLIGPMGAGKSTIGRALAKELKLEFYDSDEVIEERAG
                     ADISWIFDIEGEEGFRRREQKVIDELTQKTNIVLATGGGVVITPENRNALAGRGTVIY
                     LKTSLQQQFERTKRDTKRPLLQTEDLEGRLESLRDEREPFYDELADVSFETDKLTVKA
                     VANNIIKYLYGEV"
     gene            641..1750
                     /locus_tag="U6309_002927"
     CDS             641..1750
                     /locus_tag="U6309_002927"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEN5506333.1"
                     /translation="MAKFELYAEVDVSISGHQYPIIICRNGLIDPDLINRFITSKQVL
                     IVTNRTVAPLYLGHLQSVLPSKQCDVVILEDGEEHKNQRSLFTIYDSLIQNKHHRDTS
                     IIALGGGVIGDMAGFAASTYQRGVRFIQLPTTLLAQVDASVGGKTAINHPAGKNMIGS
                     FYQPQAVIIDLNTLKTLPEREFRAGIAEMIKYALLVGGSFFERIQDVLQQGLTVHSPE
                     LPLLIAECCQVKAKIVEQDERESGLRALLNLGHTFAHALETYTDYKKWLHGEAVAIGL
                     YCAAVLSEKKGLLDNLIVDQVEKMLIHAGLPHKIPNSIDLIQLRELMSLDKKIKNNCL
                     RFIMIKKPGACYIDDSVTEDCLHNTLINVVEGEQK"
     gene            1747..>2167
                     /locus_tag="U6309_002928"
     CDS             1747..>2167
                     /locus_tag="U6309_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN5506334.1"
                     /translation="MIDGKEPLDEGVANQPRTLFKPGAWLAKVDFINHLILFNNVLIT
                     ILSEKEGGKTCFNSLLKSHLNQQIKSVSFKAKPPFDSQDIINEFARQLHLNNKDNNIG
                     SLAAQINERKTHVLLIIDDAHHLPESFIKEVMIIIKNQ"
BASE COUNT          698 a          341 c          477 g          651 t
ORIGIN      
        1 ttgtgttatt ctatcgaggc tgttggagca gtaaatatca tgaaatcaac taagacgtgt
       61 atctaatttg ttgttataat agcatggcaa ttatgacaat tcagtagaag aggtaagtaa
      121 aaaaaaatga gcatagttaa agtacgaaat atttttctca ttggtccgat gggtgctggt
      181 aaaagtacta taggacgtgc tttggcaaag gagctcaaac tagaattcta tgactcagat
      241 gaagtaattg aagagcgtgc gggcgcagat atatcgtgga tctttgatat agaaggtgag
      301 gagggattta ggcgccgaga gcaaaaggtc attgatgaat tgactcaaaa aaccaatata
      361 gtattggcaa cgggtggtgg agtcgtcatt acgcctgaaa acaggaatgc cctggcaggc
      421 cgtggcacag tgatttatct caaaacatct ttgcaacagc aatttgaaag aacaaagcgt
      481 gataccaaac gtcctttatt acaaacagag gatcttgagg gacgcctgga gtcactcaga
      541 gatgagcgag aaccttttta tgatgaattg gcggatgtca gttttgaaac agataaactg
      601 actgtaaaag ctgttgcaaa caatataata aaatacttat atggcgaagt ttgaacttta
      661 tgccgaggtt gatgtttcga tttcagggca tcaatatcca ataattattt gccgcaatgg
      721 cttaattgat cctgatttaa ttaatcgttt tatcacttca aaacaggtgc ttattgttac
      781 taatcgaaca gtggcacctt tgtatctagg tcatttgcaa tctgttttac catcaaaaca
      841 atgcgatgtt gttattctgg aagacggtga agagcataaa aatcagcgca gtttatttac
      901 tatttatgat tctttgattc aaaacaaaca tcatagagat acctcaatca tcgctttggg
      961 cgggggagtg attggtgata tggctggttt tgcagcttct acgtaccaac gaggtgtgcg
     1021 atttattcag ctgcctacta cgttactggc tcaggttgat gcttcagttg gtggtaaaac
     1081 agcgattaat catcctgccg gcaaaaatat gattggcagt ttttaccaac ctcaagcggt
     1141 cataattgat ttaaatacct taaaaacatt acctgagcgt gagtttcgtg caggtattgc
     1201 cgagatgata aaatacgctt tgttggtcgg tggctctttt tttgaacgaa ttcaagacgt
     1261 tctgcaacaa ggattaacgg tacatagtcc ggaattacca ctattgatag cagagtgttg
     1321 tcaagttaaa gcgaaaattg tagagcaaga tgaaagggaa tccgggttgc gtgctttgct
     1381 taatctgggg catacttttg ctcatgcgct ggaaacgtat accgattata aaaagtggtt
     1441 acatggagaa gccgttgcca taggtttata ttgtgcggct gtattatcag agaagaaggg
     1501 cttgctggat aatctaattg tagaccaggt cgaaaagatg ctcatacatg ctggtttgcc
     1561 tcataaaatt ccaaattcca tagatttaat tcaattaaga gagttgatga gtttggataa
     1621 gaaaattaaa aataattgtt tacgtttcat aatgattaaa aaaccaggtg cttgttatat
     1681 tgatgattca gttactgaag attgtttgca caatacactg attaatgtgg tagaaggaga
     1741 acaaaaatga tagatggaaa ggagcctctt gatgagggag ttgccaatca gcccagaact
     1801 ctattcaaac caggagcctg gcttgcaaaa gttgatttca ttaatcattt aattctcttt
     1861 aataatgtac taataaccat tttgtccgag aaagagggcg gaaaaacttg ttttaactct
     1921 ttgttgaaaa gccatttgaa ccaacaaatt aaatcagtat catttaaggc aaaaccgcca
     1981 tttgacagtc aagatattat caatgagttt gccaggcagt tgcatcttaa taataaggat
     2041 aacaatattg gttctcttgc agcacaaatt aatgaaagaa aaacgcatgt tttgctgatt
     2101 attgatgatg cacatcactt gcctgaaagt tttatcaagg aagtgatgat tattataaaa
     2161 aatcagg
//
LOCUS       DASLOE010000281         2130 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.282, whole genome shotgun sequence.
ACCESSION   DASLOE010000281 DASLOE010000000
VERSION     DASLOE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.282"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(376..669)
                     /locus_tag="U6309_002929"
     CDS             complement(376..669)
                     /locus_tag="U6309_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN5506335.1"
                     /translation="MMSKFFNLKTGTLHSVESYAGAVKLPKNISQESIRINERILDND
                     GYPLMVPKLRRQNANQLRMFFSRESNTLLNGSYDLADRSNRDLTEVANIISYR"
     gene            758..1138
                     /locus_tag="U6309_002930"
     CDS             758..1138
                     /locus_tag="U6309_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEN5506336.1"
                     /translation="MSKAYTEKLFSYGTLRYEKVQIANFGRKMEGQEDVLQGFKLTTI
                     EIKNSAVVATSGDNFHPIISYTGNPSDKVPGIVFTISKEELAQADKYEVSEYKRVNVK
                     LNSGVNAWVYINAQDLYGNNQTKS"
     gene            1381..1623
                     /locus_tag="U6309_002931"
                     /pseudo
     CDS             1381..1623
                     /locus_tag="U6309_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215573.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          764 a          348 c          372 g          646 t
ORIGIN      
        1 tgcctatttt ttagatattc ttttattatt gttttaattt tttatgttac ctccaaagaa
       61 tttatacaag gaattgaaga tgctgggttc attgacctcg aaaaggaata ttccaattat
      121 tggagaaggt acagtattaa ccccacatag acactagata agtaatctta gctttacatt
      181 gcagcacatg aacaatttac ccgatttgga accatacaac taaccataaa atcatcagga
      241 tgaatcattc aaagcttgtt gtttatgcta tgggacttta agaataatca gcaagcaagc
      301 aaccaacaca ttcagtgaaa aactggtttg tcaggtattt ccctggttat tattctggca
      361 aatacctgat tatatttagc gataagagat tatattggca acttccgtca gatccctgtt
      421 tgaacgatca gcaagatcat atgatccatt taaaagagta ttactttctc tggaaaaaaa
      481 cattcttaac tgatttgcat tttgacgtct taatttaggt accattaaag ggtagccgtc
      541 attatccaga atcctctcat ttatccttat tgattcctga cttatgtttt taggcaattt
      601 gactgcgccg gcataggatt ctacagaatg taaggtacct gtctttaaat taaaaaactt
      661 tgacatcatt tgctcctatt ggttttataa atctcacttt gataataata agaaaatgat
      721 taaaattcac tacttttatt ttatgagggt tgattttatg agcaaagcgt acacagaaaa
      781 gttattttca tacgggacct tacgctatga aaaagtacaa attgctaatt ttggtagaaa
      841 aatggaaggc caagaagatg tattgcaagg attcaaatta acgacgattg aaattaaaaa
      901 ttcagccgtt gttgcgacaa gtggcgataa tttccatccc attatttcat atactggcaa
      961 tccctcagac aaagttccag gtatcgtatt tactatcagc aaagaagaat tagcacaagc
     1021 tgataaatat gaagtctctg aatataaacg tgtgaatgtg aaattgaact caggggtgaa
     1081 tgcatgggtt tatatcaatg ctcaagatct ctatggtaat aaccaaacaa aaagctaact
     1141 agcaatagat gttagttcag aacaaatata tagagactca aatgggtggt tctattatta
     1201 atttgtagca aatgttttat tgccgatcta caaagcgcat gtgatgttta tacgttatgt
     1261 aattaaaagc caaaaaataa aaccaaaagg cagatttttt tcacttttga tcataattta
     1321 atcaattgta tataataatt agtataaggt ttagatagta taactctgaa agaaattatt
     1381 atgacaaaac aaaagtggaa agaacttcat agttctcatc atcggggaaa aaaagattct
     1441 tatattgagt attttaaaga gcacttaatt gaacatctga ccaaagaaca tgaccctaac
     1501 ggtgaaacaa atcaacctgg taaaataata gaagccagta ggagtacgac tgggaagttg
     1561 tcaagaatat ttgatgcgta tcgtttcccg gccccaaact atgaagagtt aaacgctatt
     1621 catcaaaaac ctaatggatt aagagagcac atgcaggaaa atctaaatgg gattatccct
     1681 gttttactta atgaagatag aactgcactt catccaaagg ttattgaagc gatagggcga
     1741 gataattacg aagcaatttt aaaaaatgct caaggtaatg aacaggagat tgctctgcaa
     1801 ttcctaaagg ctgcaataat aagctatggg caaaggatgc tagataatac agatgatagt
     1861 aatctgaaaa ataaagcttt tatttcaatt atgcccgccc tggaaaaact tgccagtgaa
     1921 gtgactttgc aaggtttgcc cgaacaatct aaaaaatcaa atccactcga tatcctaaaa
     1981 atgtcacaag atctcttgaa attactcgat ggagcgaata ccgcagggat taccatacct
     2041 aaccgcaata taatgcgaga taaattgcaa actgtctctg atcttatgga tcccgacaat
     2101 gaaagttata atatgcttcc agaggaaaca
//
LOCUS       DASLOE010000282         2060 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.284, whole genome shotgun sequence.
ACCESSION   DASLOE010000282 DASLOE010000000
VERSION     DASLOE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.284"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..754)
                     /locus_tag="U6309_002932"
     CDS             complement(<1..754)
                     /locus_tag="U6309_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506337.1"
                     /translation="MRILNKRVSMQYQPIMKQIRAAISKNDADQLLNITSADWVMVYL
                     YTDHIFLNSFTVEQENFDLYDSDEILSLDFPLKESSISCFKHSEDDGYSSSMEVSHEF
                     GLDNSLMWPLFFSRANYKDGKLITQYELLQHFVHANGLYLDNEKGTFNLINEDGDVEP
                     RALIIVEQNFNLILFERKALDYYLGYANHFLVRFFELKELESPLQWSISSLQTSRIKR
                     RIYEYEDIPRFKGAEVIQTVYSIEQIEKGDPKK"
     gene            complement(757..1248)
                     /locus_tag="U6309_002933"
     CDS             complement(757..1248)
                     /locus_tag="U6309_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEN5506338.1"
                     /translation="MKQGGSYANSSGKVLEGLVEFALTKKGFTVTRYKDWKLNPSNFG
                     EELLLKNVPYEGIYKHASTTEFVLISKAYNLNTRIECKWQQVSGSADEKLPYLFLNCS
                     EKMFEPHIIILLDGGGSKTGAVNWLREECDKFNLSQSNASKRQIDLMDMTDFVKWVNT
                     VFK"
     gene            complement(1259..2059)
                     /locus_tag="U6309_002934"
     CDS             complement(1259..2059)
                     /locus_tag="U6309_002934"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019215086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEN5506339.1"
                     /translation="MRPFLKWAGGKFQIINKIRASLPKGARLIEPFVGSGAVFLNTNY
                     NKFILADINADLINLFEHLKFEKHDFIHFCKQFFTAEFNKKSTFLSLRTEFNSTTDSH
                     LKAALFIYLNRHAFNGLMRYNSSGMFNTAFGDYKKPYFPENEMLVFIQKAEKAEFKCA
                     DYSVIMGEAIKGDVVYCDPPYVPLSASANFTKYHSTAFGQEDQRRLVELAKELAAKGI
                     PVILSNHDTAFTQNLYQGSSIISFEVQRNISCNGSTRGRAREMLALFN"
BASE COUNT          672 a          409 c          290 g          689 t
ORIGIN      
        1 atttcttagg atcgcctttt tcaatctgct caattgaata gactgtttgg attacctctg
       61 cacctttaaa ccgtggtata tcttcatatt cataaattct tcgtttgatt cttgaagttt
      121 gtaaactact aatactccat tgcagaggac tctctaattc ttttaactca aaaaaacgta
      181 ctaaaaaatg atttgcataa cccaagtaat aatctaatgc ttttctttca aaaagtatta
      241 aattaaaatt ctgctcaact attattaatg cccttggttc tacatcacca tcctcgttta
      301 ttaaattgaa agtacccttt tcattatcca aatacaaacc attagcatga acaaaatgtt
      361 gtaataattc atattgggta attaatttcc catctttata gtttgcacga ctgaagaaaa
      421 gtggccacat taagctatta tctaaaccaa actcgtgtga aacttccatt gaactactat
      481 aaccatcatc ttcgctatgc ttgaagcaag aaatcgaaga ctcttttaaa ggaaaatcta
      541 aggacaaaat ctcatctgaa tcatataaat caaaattttc ttgctcaacg gtaaatgagt
      601 ttaggaaaat atgatctgta tataaataaa ccattaccca atctgctgaa gtaatattca
      661 ataattgatc agcatcattt ttggagatgg ccgctcttat ttgcttcata attggctgat
      721 attgcatgga aactctctta ttcaatattc tcatctctat ttaaaaactg tattaaccca
      781 tttaacaaaa tcggtcatat ccattaagtc tatttgccgt tttgaagcat ttgactggct
      841 taaattaaat ttatcacact cctcacgtag ccaatttact gctccagtct ttgaaccacc
      901 cccgtcaagt aaaataataa tatggggctc aaacattttt tcagaacaat tcaaaaacaa
      961 ataaggtagt ttttcatcag cactcccaga aacttgttgc catttacatt caattcttgt
     1021 atttaagtta tatgccttag atattaagac aaactcggtg gtactggcat gtttataaat
     1081 tccttcatat ggcacatttt ttaataatag ctcttcgcca aaattcgaag ggtttaattt
     1141 ccaatcctta taacgagtta cagtaaagcc cttttttgtt agagcgaact ctactaatcc
     1201 ctcaagaacc tttccgctac tatttgcata tgaaccaccc tgcttcatgg caacacccct
     1261 aattaaatag ggcaagcatt tcacgtgctc gtccccttgt gctcccgtta caactgatat
     1321 tccgttgcac ctcaaaactg ataatgcttg atccttgata taaattctga gtaaaagccg
     1381 tatcatgatt agataaaatt acaggaatcc ccttcgcagc cagttcttta gctaactcaa
     1441 ctaatcgtct ctggtcttct tgtccaaaag ctgtggaatg atattttgta aaattggctg
     1501 atgcagataa tggaacatat ggggggtcgc aataaactac atctccttta atagcttctc
     1561 ccataattac gctataatca gcacatttaa attctgcttt ttccgctttt tgaataaaaa
     1621 ccaacatttc attttctgga aaataaggtt ttttataatc cccaaaagcc gtgttaaaca
     1681 tgccagaact gttgtacctc atgaggccat taaaagcatg acgattgaga taaataaata
     1741 gtgcagcctt caagtggcta tctgtagttg aattaaattc tgtacgcaat gataggaagg
     1801 ttgatttttt attaaattca gcagtaaaaa attgcttaca aaaatggata aaatcatgct
     1861 tttcaaactt caaatgttca aacaagttaa ttagatcagc attaatatca gcgagtataa
     1921 acttgttata gttcgtattt aaaaaaactg ctcctgaacc tacaaacggt tcaatcaaac
     1981 gagccccttt aggtaggctc gcacgaattt tatttattat ctggaactta ccgccggccc
     2041 attttaaaaa tggtctaatg
//
LOCUS       DASLOE010000283         2053 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.285, whole genome shotgun sequence.
ACCESSION   DASLOE010000283 DASLOE010000000
VERSION     DASLOE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.285"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            158..1027
                     /locus_tag="U6309_002935"
     CDS             158..1027
                     /locus_tag="U6309_002935"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HEN5506340.1"
                     /translation="MSDYLDKTASLTPATKRIVCNKATEYPHTGSYNQVATHGTYLCR
                     RCGLALFRGVSQFSSGCGWPSFDDEIANAVARKPDADGQRTEILCARCDAHLGHVFTG
                     EYMTYKNLRHCVNSASLDFVADNEVLDTEEAILAGGCFWGVEYYLKRIPGVLKVESGY
                     TGGHVSEPSYEQVCSGNSGHYEAVRVVYDAAKTNYHDIVKRFFEIHDPTQANGQGPDL
                     GHQYLSAVFYFNQEQRQEAELLIQLLKKRGYQVVTQLLQAQPFWPAEDYHQDYYFKHH
                     KIPYCHKSVDRFG"
     gene            complement(1051..2016)
                     /locus_tag="U6309_002936"
     CDS             complement(1051..2016)
                     /locus_tag="U6309_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506341.1"
                     /translation="MAFSKSETTVDKFSLDEFPAFSAHDLEMAFSLKKKYRESKKSDH
                     PFTGFVCDYERCEKFYDFIYNSIHSPKLFENNFKVQIAYLDREKSGVRHWSLIELQYS
                     IEKGNNTLKILVLDSLGAQLSYNTAQILANEVGFQDLLDENEDLSIKIYIPDVKLQHS
                     RAGCATFTMDCASMLSTQDKYENIYDFMDKQAEVSDKVKIAKLDTDFPVHCASLPPRI
                     LRVAQSRAIITDDKVLNSTPLINSKEETFKHSLLKHCSFFEDQPQTPENIKRNERIMK
                     KALKWRNTIDLKMKTSSAEEFENTCAIDDLEEYANSCLPSKSVQL"
BASE COUNT          571 a          383 c          445 g          654 t
ORIGIN      
        1 caaaaacagc taatcctgct ataatttttg aacaaaaggt gagctcctgt tggcaggatt
       61 tttcactttg aatcgttcac tgacggcttc tgagactgat ggcatagggg gtgttcaggt
      121 gattttctca tgagtcgagt tttcaaagga gcatgaaatg agtgactacc tcgataaaac
      181 cgcaagctta accccagcca ctaaaagaat agtgtgcaac aaagctactg aatatcctca
      241 tactggctct tataatcaag tagcgactca tggcacgtat ttatgcaggc gctgtggcct
      301 ggcgttgttt cgtggtgtca gtcaatttag ctcaggctgt ggctggccca gttttgacga
      361 tgaaatagct aacgctgtgg caaggaaacc ggatgcggat ggtcagcgca ccgagatatt
      421 atgcgctcgt tgtgatgctc acctggggca tgtgttcaca ggggaataca tgacgtataa
      481 gaatttacgt cattgcgtta attccgcttc attggatttt gtcgcggata atgaagtact
      541 ggatacagaa gaagccatcc ttgcaggggg gtgtttttgg ggagtggaat attatttaaa
      601 acgaattccc ggagtgttaa aagtcgagtc gggttataca ggtggccatg tttcagagcc
      661 aagttatgag caagtatgca gtggtaattc agggcattat gaggctgtgc gcgtcgtcta
      721 tgatgctgct aaaaccaatt atcatgacat tgtaaaacga ttttttgaaa ttcacgaccc
      781 aactcaggcc aatggacaag gtcccgactt gggccaccaa tatttaagtg ccgtgtttta
      841 ttttaatcag gaacaacgcc aggaagcgga gttattgatt cagttattaa agaaaagagg
      901 gtatcaggtt gtaacccaat tattgcaagc acaacctttt tggcctgctg aagactatca
      961 tcaagattac tattttaaac accacaaaat tccttattgc cataagtcag tcgatcgttt
     1021 tggctagttg ttccggaaga tttaatctca tcatagttgc acggatttag aaggcagaca
     1081 actattggca tattcttcaa gatcatctat tgcgcacgtg ttttcaaatt cttctgcact
     1141 gcttgtcttc attttcaagt ctatggtgtt tctccatttg agcgctttct tcatgattct
     1201 ttcgttgcgc tttatgttct ctggtgtttg gggttggtct tcaaaaaaac tacagtgctt
     1261 tagaagagag tgcttgaagg tttcctcttt agaattaatt aatggggtag aattcaggac
     1321 tttatcatct gtaatgatgg ctcggctttg tgcaacccgt aatatccgag ggggcaaact
     1381 ggcacaatgc accggaaaat cagtatcaag ttttgcaatt tttaccttgt cacttacctc
     1441 agcttgttta tccataaaat cataaatatt ttcatattta tcctgggtag acaacatgct
     1501 ggcgcaatcc atagtaaagg tggcgcaccc tgcgcgcgag tgctgtaatt taacatccgg
     1561 gatgtagatt tttattgaaa gatcttcgtt ttcatcaagc aaatcctgga atccaacttc
     1621 attcgctaat atttgagcgg tgttatagga taattgtgct cccaaactgt caagcaccaa
     1681 aatttttaaa gtattattgc ccttttcaat ggaatattgt agctcaatta atgaccagtg
     1741 gcgcacaccg gatttttctc tatccagata ggcaatttgt actttgaaat tgttttcgaa
     1801 taattttggt gaatgaatgc tgttgtaaat aaaatcataa aatttttcac agcgttcgta
     1861 atcacagaca aaaccggtaa aaggatgatc tgatttcttt gattcccgat attttttctt
     1921 taaactaaag gccatttcca aatcatgtgc ggaaaatgca gggaattcgt ctagagaaaa
     1981 tttatcaacg gttgtttctg atttgctaaa ggccatataa agaatcccat ttgtttgtat
     2041 ttttattata tga
//
LOCUS       DASLOE010000284         1934 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.286, whole genome shotgun sequence.
ACCESSION   DASLOE010000284 DASLOE010000000
VERSION     DASLOE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.286"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1868)
                     /gene="ravR"
                     /locus_tag="U6309_002937"
     CDS             complement(<1..1868)
                     /gene="ravR"
                     /locus_tag="U6309_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector RavR"
                     /protein_id="HEN5506342.1"
                     /translation="MLSQGVRRMGKEKRELEASYIPLPRKGRFWIHSESCYTEEELNF
                     REWILKIRSNSGIHPDYLNNPSSWFASDDQALVNLYEHAWFYSNAYKPRHVLSLDDFH
                     VQPIDNQRLQWPQLYDLMQKVGDYLLLRNKELSPEHLNQPMNFVLLDVNNILKGLSQN
                     NNIQQVKDQLESVTRYLRTIEKNISPLIGSDRLFLANFRKTVDTKIHPHLDHMLETQL
                     LRDKLGELSKDIRKLSSERNRILHYALNANPVNPHSYEFELDKISDSASYPTQAAKHC
                     AEKSNELVADPLPILHLTKEQLSDCPHFKLITMKDEILDHYAKAVSDLNELERFQKVI
                     QEIMDLLGQAGEVYTVHQFKNQMQSLLTQIDSFIDASSVHIDEIIDANTQAYHKAIQE
                     EQNLPFWKNWLTNEKEKLKIFIKNQDTLSQFPSSSSDLKKTNKALKNRVNEVVSHLNQ
                     PKLKETSFEAIAGQTQALNKLMLSMHDWMKIQHELKGLPLPQPPEPLKIMPTPIPTVD
                     VFSSPPPPLHAYKPLFTALPGNTTAPCTPDNNQCKTTPSSPTQNSLGYLGLIALIPIG
                     VIALYLVIHWINKKDAQIRVSGTEEEFNKIKEKFEDLLAELKGFEHSEEIDLSDDY"
BASE COUNT          543 a          328 c          403 g          660 t
ORIGIN      
        1 taatagtcat ctgagagatc aatctcttcc gaatgctcga aacctttaag ttcggctaat
       61 agatcttcaa atttttcttt tattttattg aactcttctt ctgtgccaga tactcgtatt
      121 tgagcgtctt ttttatttat ccaatgaata accaaatata aggctatgac accaataggt
      181 atcagggcaa ttaatcctaa ataacctaaa gaattttgtg tgggtgaact gggggttgtt
      241 ttgcattgat tattgtcagg cgtacaagga gctgttgtat tgccaggcag ggcggtaaat
      301 agaggcttat atgcatgaag tggaggcggg ggagaagaga acacatcaac tgttggtata
      361 ggagttggca ttatcttgag gggttcaggt ggttggggaa gtggaagccc tttcaattca
      421 tgttggattt tcatccagtc atgcattgat aacatcaatt tattaagtgc ttgagtttgt
      481 ccagctattg cttcaaagga ggtttctttt aatttaggct gatttaaatg gctaacgact
      541 tcattgaccc ggttttttaa agccttgttt gtttttttga gatcagaaga actggatgga
      601 aattgagaga gtgtgtcctg atttttaata aaaatcttta gtttctcttt ttcattagtt
      661 aaccaatttt tccagaaggg taaattttgt tcctcttgta ttgccttatg ataggcttga
      721 gtgttcgcat caattatttc atcaatatgc actgaggagg cgtcaataaa gctgtcaatt
      781 tgggtcagta gggattgcat ttgatttttg aattgatgaa cagtatagac ttcgccagcc
      841 tgccccagaa gatccatgat ttcctggatg actttctgaa atcgttccaa ctcgttaaga
      901 tcagagactg ctttggcata atgatcaagg atctcatctt tcatcgtgat taatttgaag
      961 tgaggacaat cacttaattg ctctttagtt aaatgcagta taggcagagg atctgcgact
     1021 aattcatttg atttttcagc gcagtgtttg gctgcttggg ttggataaga agcggagtca
     1081 ctgattttat ccagttcaaa ttcataagag tggggattta ccggattggc attaagagca
     1141 taatgcaaga ttctatttcg ctcactagat aattttctga tgtcttttga taactctccc
     1201 aatttatctc taagtaattg agtttccagc atatggtcca gatgagggtg aattttagta
     1261 tccactgttt tgcgaaaatt ggctaaaaat aatcggtccg aaccaatcag tggtgaaata
     1321 ttcttttcaa tagtacgcag gtagcgagta actgactcaa gctgatcttt tacttgttgt
     1381 atgttgttgt tctgacttag cccttttaag atattgttca catcaagaag gacaaaattc
     1441 atgggttggt ttaaatgttc gggggataac tccttatttc ttaataaaag atagtcaccc
     1501 actttttgca ttaagtcata aagttgtggc cactggaggc gctgattatc aatgggttgg
     1561 acatggaaat catcaaggct taacacatgt ctcggcttgt aggcatttga ataaaaccag
     1621 gcatgttcat aaagattaac caaagcttga tcgtcgctgg caaaccagga gctgggatta
     1681 ttaagataat ccggatgaat ccctgaattg gaacgaattt ttaaaatcca ttctctgaaa
     1741 tttaactctt cctctgtgta gcaactttca gaatgaatcc aaaagcgtcc ttttctaggt
     1801 aacggtatat atgaagcttc aagctctctc ttttcctttc ccatccttct cactccttga
     1861 gaaagcaaaa aaaaacctaa ggtaattcca gattaacact agattagcaa tagctttttt
     1921 tgctgccgat taat
//
LOCUS       DASLOE010000285         1910 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.287, whole genome shotgun sequence.
ACCESSION   DASLOE010000285 DASLOE010000000
VERSION     DASLOE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.287"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..639)
                     /locus_tag="U6309_002938"
     CDS             complement(<1..639)
                     /locus_tag="U6309_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506343.1"
                     /translation="MKKCVLIVDPFSSGALYAEALIKRGIVPVAILSHYPIIEVYRDS
                     FKSSDFKYIFTYSENADFYEELNIFLRDKELIVVLAGAESGVELADKLSERYCIDHSN
                     GTALSSCRRNKYEMVQKLGQLHIDVPLTIKSNSSDEFLTWINNNNLFTKGVVIKPLKS
                     AGTDSVHACFNEQELIEVVNQNIGKINQLNFKNDDLIAQEYLIGTEYVVDSTS"
     gene            complement(674..1882)
                     /locus_tag="U6309_002939"
     CDS             complement(674..1882)
                     /locus_tag="U6309_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506344.1"
                     /translation="MVRHLKLIIILAFTLAGDILFGLALIWRVLALDGSAKTLGLFLC
                     VITLVTFALQKSSVGFRRMLEKTPRRSFMWIRGVGVGFSIIFIPLLWHETTWLLYLVG
                     AIATIINFLSILTLEAMMAHEVLEKRISSNKASRILQIAILTSSFLGASLLGTILALG
                     NIVAVLLFCIATYLVGAICFSTQSDVGIQKISQVQTAKTASNQKMTYGNNLSVLRLAF
                     IGLTLLMTQISAFNFLLPLIAQHEKFWTASQYGFIDAAAELGGLLATIIMFEKGIFSK
                     LWIFAFLGIALVDILFFAVTDIYLISTATLFLGFCVSCFRIKQREYIYDSVSNTEEIL
                     EWTGRITAMTAIIKALTPLGLAFFISQPSFDLMIIGIVVGTVMLIVTSVLYFYTNKEF
                     KQQIPSYSAV"
BASE COUNT          666 a          356 c          287 g          601 t
ORIGIN      
        1 acttgtagaa tccactacat attctgtgcc aattaagtat tcttgagcta ttaaatcgtc
       61 atttttaaaa ttaagttgat taatcttgcc tatattttga tttaccacct caatcaactc
      121 ctgctcatta aagcaagcat gtacactatc agttcctgca ctttttaatg gtttaataac
      181 aacaccttta gtaaacaaat tattattatt aatccacgta agaaactcat cagatgaatt
      241 acttttaatt gttaaaggaa catcaatatg aagttggcct aatttctgta ccatttcata
      301 tttatttctt ctacaactgc ttaaagctgt cccatttgaa tggtcaatgc aatatctttc
      361 agacagctta tctgctaatt caactccaga ctcagctcca gctaaaacta ctattagctc
      421 tttatctctt aaaaatatat ttaattcttc atagaagtca gcattttcac tgtaagtaaa
      481 aatatattta aaatcagaag acttgaacga atctctataa acttcaatta ttggataatg
      541 tgataagatg gcaacaggaa caatgcctcg ttttatgagt gcttcagcgt atagtgcacc
      601 tgaagaaaag gggtcaacaa ttaaaacaca ttttttcata tcaatctcac tttatataaa
      661 tttaatttat taactaaacc gcactatagc ttggtatttg ttgtttgaac tctttattcg
      721 tatagaaata caatacggat gtaactatta acattaccgt acccaccaca attccaatta
      781 tcattaaatc aaacgagggt tgagaaataa aaaaagctaa gccaagcggc gtaagagctt
      841 ttataatggc tgtcattgca gtgatgcgtc cggtccattc gagtatttcc tcagtatttg
      901 atactgagtc gtagatatac tctcgttgtt ttatccgaaa acaactgaca caaaatccaa
      961 gaaataatgt agctgtgcta ataagataaa tatctgtcac tgcaaaaaat aaaatatcga
     1021 ctaatgcaat gccgagaaat gcgaatatcc atagctttga aaaaattcct ttttcaaaca
     1081 taataatcgt agctaagagc ccacctaact ctgctgcagc atcaataaat ccatattgag
     1141 aggccgtcca aaatttctca tgctgagcaa ttaaaggtaa caagaaatta aaggcagaga
     1201 tttgtgtcat taacaatgtt agaccgataa atgccagtct taaaactgat aagttatttc
     1261 cataagtcat tttctgatta gaagctgttt ttgcagtctg gacttgacta attttttgga
     1321 tgccgacatc tgactgtgtt gaaaaacata ttgcgccaac taaataagtc gcaatacaaa
     1381 aaagtaaaac agctacaata ttaccaagtg ctaatattgt tccaagcaaa ctggcgccca
     1441 aaaaagatga ggtcaggata gctatttgta gaatacgcga ggctttattc gagcttattc
     1501 ttttttcgag cacttcatga gccatcattg cttcaagggt taagatgctc aaaaaattaa
     1561 taattgttgc tatcgcacct actagataaa gaagccacgt agtttcatgc cataatagcg
     1621 gtatgaatat aatagagaac ccaacgccta ctcctcttat ccacataaaa gagcgacgtg
     1681 gtgttttttc tagcatcctt ctgaagccaa cgctagattt ttgtaatgca aaagttacaa
     1741 gagtaattac acaaagaaaa aggcctaatg tttttgctga accatctaaa gctaaaactc
     1801 tccagataag cgccagccca aataatatgt cgcctgcaag agtaaatgct aatatgatta
     1861 taagttttaa atgacgtacc ataataataa tccttataat aatcctttta
//
LOCUS       DASLOE010000286         1887 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.288, whole genome shotgun sequence.
ACCESSION   DASLOE010000286 DASLOE010000000
VERSION     DASLOE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.288"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            169..471
                     /locus_tag="U6309_002940"
     CDS             169..471
                     /locus_tag="U6309_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN5506345.1"
                     /translation="MGLLMNTFNIEEASKFLGAHRETIRRMAASGELPGVKIGRGWRF
                     IEQDLAMYMRNKYSTCDASWGVDIGDNKWHSRKKMGFGGSTSPSVTSVYAEALGLK"
     gene            384..1388
                     /locus_tag="U6309_002941"
     CDS             384..1388
                     /locus_tag="U6309_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN5506346.1"
                     /translation="MALTKKNGFWWIDVTFGDKRIRRSTRTKVKQQAQQFHDNFLQDL
                     WSNKHTKLPQQKSWVDAVVRWLEESAHKRSLVTDKFHLAFLDPYLGQKTLDEINAELI
                     EFIAQEKERTNVTPTTVNRMLEIIRAILNKAHKEWQWINTMPLFRMRKVENKRIRWLS
                     RGQANKLIQELPNHLKDMAAFTLATGLRQSNVTQLQWSEVDLIRKHALIHPDQSKTKK
                     AIPVPLNSQAIVIIEKQVGLHPTFVFTYFGKPVTRCNNHAWQKALIRAGIKDFRWHDL
                     RHTWASWHVQNGTSLQELQQLGGWSEYEMVLRYAHLSSDHLKVAAERITKNSGTFSSL
                     "
     gene            complement(1453..1529)
                     /locus_tag="U6309_002942"
     tRNA            complement(1453..1529)
                     /locus_tag="U6309_002942"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1493..1495),aa:Arg,seq:tcg)
     gene            complement(1588..1663)
                     /locus_tag="U6309_002943"
     tRNA            complement(1588..1663)
                     /locus_tag="U6309_002943"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1628..1630),aa:Lys,seq:ttt)
     gene            complement(1719..1794)
                     /locus_tag="U6309_002944"
     tRNA            complement(1719..1794)
                     /locus_tag="U6309_002944"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1759..1761),aa:Lys,seq:ttt)
BASE COUNT          592 a          345 c          420 g          530 t
ORIGIN      
        1 tactctaaaa gacattgctg ctttactaaa ttgctcaata agacaattac gttctattta
       61 tttcgatgag ttaatgccaa ctaatctata ttgcgaacgg gatcttgtga ggctttatca
      121 tttaattctt gaactcaata ctaataaata tgtgcaccag ggttataaat ggggttgctt
      181 atgaatactt ttaatattga agaggcttca aagtttctag gcgcccacag ggaaactatc
      241 cgacgaatgg ctgcatcagg tgaactacct ggagtaaaga tagggcgagg ttggcgtttt
      301 atagaacaag atcttgcgat gtatatgcga aacaagtact ctacttgcga tgcatcgtgg
      361 ggtgtcgata taggagataa taaatggcac tcacgaaaaa aaatgggttt tggtggatcg
      421 acgtcacctt cggtgacaag cgtatacgca gaagcactag gactaaagta aaacaacaag
      481 cgcaacaatt ccatgataat tttctacaag atctatggag taacaagcat accaaattac
      541 cccaacagaa atcgtgggta gatgctgttg tgcgatggtt agaggaatca gcgcataaac
      601 gaagtttagt tacggataaa tttcatttgg cctttctgga cccttatctt ggccagaaaa
      661 cgttagatga aattaacgcc gaattaattg aatttattgc gcaagaaaaa gagcggacta
      721 atgttactcc aacgacggtc aaccgaatgt tggaaattat acgtgctatt ttaaacaaag
      781 ctcataagga atggcagtgg ataaacacca tgccattatt cagaatgaga aaagttgaaa
      841 ataaacgtat acgttggttg agtaggggac aggctaataa attgattcag gagctaccaa
      901 atcatttgaa agatatggca gcttttactt tggcaactgg tttacgccaa tctaatgtta
      961 ctcaattgca gtggagtgaa gtggatttaa ttagaaaaca tgcactcatt caccctgatc
     1021 aatctaaaac aaagaaggct atccctgttc ctttaaactc tcaggcaatt gtcattatag
     1081 aaaaacaagt gggcttacat cccacatttg tatttaccta ttttggtaag cctgttacgc
     1141 gatgtaataa tcatgcttgg caaaaagcgt tgattcgggc aggaatcaaa gattttcgct
     1201 ggcatgactt gcgtcacact tgggcaagtt ggcatgtgca aaatggtacc tcattgcaag
     1261 aattacagca attaggtgga tggtcagaat atgaaatggt gttacgttac gcgcatcttt
     1321 caagtgacca tttaaaggtg gcagcggaaa gaattacaaa aaattctggc acgttttcgt
     1381 cactgtaact tggtttttac ggcacgtttc gatccaggca accgttaact acctgatttt
     1441 aaagggttta aatggtgcgc tcggagggac tcgaaccccc gacaccttgg ttcgaagcca
     1501 agtactctat ccagctgagc tacgagcgca atggggtgtg gatgatcatt ttatttctgg
     1561 tctgtttatg caggagggag accagaatgg tgggccgtat aggattcgaa cctatgacac
     1621 agaggttaaa agcctcctgc tctaccacct gagctaacgg ccctttcagg gtataaccag
     1681 caaaatacaa tagttgatgg tataggttta ttaaaaactg gtgggccgta taggattcga
     1741 acctatgaca cagaggttaa aagcctcctg ctctaccacc tgagctaacg gccctaaaga
     1801 ggctgaaatc ataccggatt agagcagaat ttcaagtctt tttagagatt attttttgtt
     1861 taacaaacaa gatgtgttat ttatatg
//
LOCUS       DASLOE010000287         1837 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.289, whole genome shotgun sequence.
ACCESSION   DASLOE010000287 DASLOE010000000
VERSION     DASLOE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.289"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            70..543
                     /locus_tag="U6309_002945"
     CDS             70..543
                     /locus_tag="U6309_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506347.1"
                     /translation="MRSRTEKLSSKRSIINHDHLITDEACMIGIYREGGDLSIYAESS
                     EGIEHFSINRGNSLDMVILPHTLVGTWGHHQLLRTVEQAAQIRALRIRSHDYIEQSVT
                     RKELEQFKEVFMLACKEEISNQPEIEDYLQTALGKLHGEPEMTYKPKFTEQCSIL"
     gene            722..1639
                     /locus_tag="U6309_002946"
     CDS             722..1639
                     /locus_tag="U6309_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN5506348.1"
                     /translation="MDKLNLNLLRALYALLTCRHLTLASKKMGISQSSMSIYLKQLRE
                     HFHDDLLVRGQGNIMQLTPLANSLIGKARQALSLIETLFNTIETFNPLISERSFSIGM
                     TDLMSILLTPSLIKRLEQEAPGVKLNIKHPSYLNTVEVFESGDIDLMIGMFERVPEGL
                     KQQKLFQDEAVIVGGNNHPGIQKGKICIEELFKYPLIQFSLRETPFYNYFDRYLRQLG
                     HDKRVSISLGQGIMPLFVLTDSHYLTLSIRKVAEKLSHLIPLSIASVPFEVDSFNCYQ
                     YWHQKDDADPGHRWLREMVLSIAKKSYLK"
BASE COUNT          637 a          325 c          326 g          549 t
ORIGIN      
        1 caataagatt aaaaattatt catattgaag cgatgaattt attaaacaat tgtaaaatag
       61 gagttatcca tgcgtagcag aacagaaaaa ttaagcagta aacgttcaat catcaatcat
      121 gatcatttaa taactgatga agcctgtatg ataggaattt atagagaagg gggtgattta
      181 tcaatttatg ctgaatcatc tgaaggaatt gaacatttct ctataaatcg gggcaacagt
      241 cttgatatgg tgatattacc acatacttta gttggaactt gggggcatca tcaattactt
      301 cgtacggtgg agcaggcagc acagattcga gcattaagaa taagatctca tgattacatc
      361 gaacaatcag tcacccgaaa ggaacttgag caattcaaag aggtatttat gctcgcatgc
      421 aaagaggaaa taagcaatca acctgaaatt gaagattatc tccaaaccgc tttgggtaag
      481 ttacatggag aaccagagat gacttacaaa ccaaagttca cagagcaatg cagcattctc
      541 taatttttat tagaataatc agagaaaagg ctcgagattc aaaatcttcg agctttttta
      601 atatcctatt agtaccaggc ttatttgcaa actgtatcgt tctcaactaa cctcaaacaa
      661 aaagccgtta aaaatagagt catttaaaac ctattagggt actatatacg gatgatgttt
      721 tatggataaa cttaatctca atttacttcg cgctctgtat gcactattaa cctgccgtca
      781 tctaacactt gctagcaaaa aaatgggtat aagccaatcc tcaatgagta tttatttgaa
      841 gcaactgcgt gagcatttcc atgatgattt gcttgtacgt ggccaaggca atattatgca
      901 attaactcct ttagctaact cgttgatcgg taaagcccga caagctctct cactgattga
      961 aacactattt aatactatag agacctttaa ccccttaatt tcagaaagaa gtttctcaat
     1021 tggcatgact gatttaatgt ctattttgtt gactccaagt ttgattaaac gattggaaca
     1081 agaggctcca ggagtcaagc taaatatcaa acatcccagt tatttaaata cggtagaagt
     1141 gtttgagtcc ggtgatattg atttaatgat tggtatgttt gagagagtcc cagaaggctt
     1201 aaagcaacaa aaattgtttc aggatgaagc cgtcatcgtt gggggaaaca accatccagg
     1261 aatccaaaaa gggaaaattt gcattgaaga actatttaag tatccattaa ttcaattttc
     1321 attaagagaa actccttttt acaattattt cgatcgctat ttacgtcaac tgggacatga
     1381 caaacgggtt tctattagct taggacaggg gatcatgcct ttatttgttc ttactgactc
     1441 tcattatctt acattaagta ttcgaaaagt agctgaaaag ctttcccatt tgataccatt
     1501 aagtattgcc tctgtaccct ttgaggttga cagttttaat tgctatcaat attggcacca
     1561 aaaagatgat gctgatcctg gccatcgttg gttacgggaa atggttctca gtatagctaa
     1621 gaaatcttat ctaaaataat cagttaaaac ccactctacg aactagctat agattgatat
     1681 aaaacacatg tcttaattag ataatcacag aaaaagtctt ttttaataga ccatgaacca
     1741 ggactggtat tactagcaaa tcgcaaataa taaaaactaa atggcgataa aatgcaccct
     1801 gaaaccagca ttataaaatg tccccaatgc actaaac
//
LOCUS       DASLOE010000288         1834 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.290, whole genome shotgun sequence.
ACCESSION   DASLOE010000288 DASLOE010000000
VERSION     DASLOE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.290"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            53..946
                     /locus_tag="U6309_002947"
     CDS             53..946
                     /locus_tag="U6309_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN5506349.1"
                     /translation="MVANFVTVVIVNYNSGEMLSKCVKHLLKSTVQVQIIISDNASSD
                     NSLALLEQLELNKQTITIHRNNSNYGFSYANNAVFTKISTPFILYLNPDCFVEKDTIE
                     NLLHTMHQHNDAGMVGCLVNNMDGSVQTGCCGLTPTPARVLNQLLKLSKIFPNNPKFG
                     GYLFSKEKLPTKPTEVELISGSCMFVRKKAIDEVGLLDDNYFLYCEDYDWFYRFTQSN
                     WKIIFNPNTKVTHIKSFSTKQIPFKVLLYKAKGMWRFYNKFFKQNSSPATTIIIRVGI
                     IARFFSLSGLLLFKKLLRTTN"
     gene            complement(1115..>1834)
                     /locus_tag="U6309_002948"
     CDS             complement(1115..>1834)
                     /locus_tag="U6309_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN5506350.1"
                     /translation="MIQHTPFNRIFEFLPTIPPLAQISPLWSIAVEWWLYTLFGIAFF
                     FHKSSFATRFIMSLLIIPALLVAGYFTLKEYVALVWFLGSGCAYHFCNINKKYNNNAI
                     LMLSLITGTAFLVRFYILKHSIMNMYDLQLVIPGCIFLYSLLLLLSANKLNKKIELIS
                     AFLAFISYTLYLSHEPIRRVVSTFIEPSNLKRGFFICGVCIACATLIAYLLENKHLLV
                     REWMKNKFLQKNTSNKLAYIT"
BASE COUNT          645 a          311 c          289 g          589 t
ORIGIN      
        1 cgagaattct ttctaacttt caattactaa aaatcttgaa aaaaggcttg ccatggtagc
       61 taatttcgta acagttgtta tagttaatta taattcgggc gaaatgctta gtaagtgcgt
      121 aaagcatcta ttaaaaagta cagttcaagt tcaaattatt atttcagata atgcctcctc
      181 tgataatagt ttagcgttat tagaacaact tgaattaaat aaacaaacta ttactattca
      241 cagaaataat tccaactatg gattttctta cgccaataat gcagttttta caaaaatctc
      301 aaccccattt atactctatc ttaaccctga ttgtttcgtt gaaaaggata ccattgaaaa
      361 tcttttgcat acaatgcacc aacacaatga tgctggaatg gttggatgtc tggtaaataa
      421 catggatggt agcgtgcaaa ctggctgttg tggacttact ccaaccccag ccagggtttt
      481 aaatcaacta ttgaaactat caaaaatctt cccgaataac cctaaatttg gggggtactt
      541 attttcaaaa gaaaagctcc cgacaaaacc aactgaagta gagttgattt cagggtcttg
      601 tatgtttgta cgcaaaaaag caatcgatga agttggcctt ctggatgata actatttcct
      661 atattgtgag gactacgatt ggttttatcg atttacacaa agtaattgga aaattatttt
      721 taatccgaat actaaagtta ctcatataaa aagtttttcc accaaacaaa ttccatttaa
      781 agtactgttg tataaagcaa agggcatgtg gagattttat aataaattct ttaaacaaaa
      841 ttcttctcca gctaccacta ttattattag agttggaatt atcgcgagat ttttttcttt
      901 aagtggtttg cttcttttta agaagctgtt gcgtactacc aactagaaaa ttcaccgcct
      961 ctaactccac gtaaccgtac acgcaatgga gtcaacttaa ggtgatgtct tatttagcaa
     1021 acccgccaaa aggactgatg ccgcttgcgg ttttcccatt ttatcctaat aattaacagg
     1081 tttacccaaa agacaaataa atcggataat ttgcttacgt aatataagct aacttatttg
     1141 atgtgttttt ttgcaaaaat ttatttttca tccactcacg aaccagtaga tgtttattct
     1201 ctagtaaata agcgatgaga gttgcacaag caatgcaaac tccacagata aaaaaacctc
     1261 tctttaaatt agatggttca ataaatgttg atactactct tcttattggc tcatggctca
     1321 aatatagtgt ataggaaata aacgcaagga atgctgatat taattctatt tttttgttta
     1381 gtttgttagc tgacagtaat aacagcagtg aatataagaa gatacaacca gggattacta
     1441 actgtaaatc atacatattc attattgaat gcttcaatat atagaatcta actagaaaag
     1501 ctgttcctgt gattaggctt agcattaaaa ttgcgttatt attatacttc ttatttatat
     1561 tacaaaaatg atatgcgcac ccagaaccca gaaaccatac taaagcaaca tactctttta
     1621 atgtgaagta tccagcaact agtaatgcag gaataatcaa taaactcatt ataaacctgg
     1681 tagcaaaaga gcttttatga aaaaaaaagg ctattccaaa caaagtatat aaccaccact
     1741 caactgctat tgaccacaat ggggaaattt gagctaaagg tggaatagta ggtaaaaatt
     1801 caaaaattcg attaaaaggt gtatgttgta tcat
//
LOCUS       DASLOE010000289         1808 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.291, whole genome shotgun sequence.
ACCESSION   DASLOE010000289 DASLOE010000000
VERSION     DASLOE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.291"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            126..485
                     /locus_tag="U6309_002949"
     CDS             126..485
                     /locus_tag="U6309_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506351.1"
                     /translation="MSNKAKNTHLKNGDSQTKQAYTNQASPLFFSQKQITPSSGTSET
                     KKTQEGKQPTQEISLETTTDILLAAILQELKTQNMIELMKLKAQQEQEQEELQAIETM
                     KEKDDARFNEIRQTMYA"
     gene            complement(613..1683)
                     /locus_tag="U6309_002950"
     CDS             complement(613..1683)
                     /locus_tag="U6309_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN5506352.1"
                     /translation="MKTGRFDQARADAFAERLLSVLNDAALTLMISIGHRTGLFDVMD
                     ELDFASSEVIAEAAGCNERYVREWLNALVVGGIVDYDAHAKAYQLPAEHASFLTRKSS
                     PQNMAVVAQFIPVLAYVENGVVDAFKHGGGVPYEAYHRFHEVMAEESGQTILSSLTDK
                     ILPMVHGLVDKLNQGIQVLDIGCGYGRSLILMAKTYPQSQFTGYDLCDETIAHAVHSA
                     KENKIQNIHFEKMDLSVWDEQEKYDLITSFDVIHDQAKPAQVLSNVFKALKPRGIFLM
                     QDIKASSDVAGNVHHPLGALIYTISCMHCMTVSLSQNGLGLGAAWGEELAEKMLVEAG
                     FGKIEKHYLEHDIMNTYYVMRK"
BASE COUNT          609 a          414 c          324 g          461 t
ORIGIN      
        1 acctgatctt aatgatctat attaacgaca aaaacataaa atttataaat attgaaaaaa
       61 acatttgcat ttaactcaag atttgcatta atctgaatgg gactcacaag gaaaaggaga
      121 tcaccatgag taacaaagcg aaaaacactc acctcaaaaa tggagatagt caaaccaaac
      181 aagcttatac caatcaggct tcaccacttt tttttagtca aaagcaaatc accccctcat
      241 cgggaacatc ggaaacaaaa aaaacacagg aaggaaaaca acccactcaa gaaatcagcc
      301 ttgagacaac cacagacatt ttattagccg ctattttgca agaattaaaa acgcaaaata
      361 tgattgaact catgaaactc aaagctcaac aggagcagga gcaagaggaa ttgcaagcca
      421 ttgagacaat gaaagagaaa gatgacgccc ggtttaatga aatccggcaa actatgtatg
      481 cctgatggac aggacatact tcttaaccaa ttaacagatc actgttaatt ggtttttcca
      541 ccaaacagag taaatgttac ctcaaaagaa aatactgtaa ttttattaga cctcttgcat
      601 aatctgttta ttttatttac gcatcacata gtaggtattc ataatgtcat gttccaggta
      661 atgcttttca atttttccaa aaccagcttc aaccagcatt ttttctgcaa gctcctctcc
      721 ccaggccgcg ccaagaccta agccattttg tgagagagat acagtcatac aatgcataca
      781 ggagattgtg taaataagcg cccccagagg atgatgaaca ttccctgcca catcactgga
      841 ggctttgata tcctgcatca ggaaaatacc tcgaggcttc aatgccttga atacgttgct
      901 cagtacctgc gctggcttag cctggtcatg aatcacgtca aagctggtaa taagatcata
      961 tttctcttgc tcatcccaga ccgacaaatc cattttttca aaatggatat tttgtatttt
     1021 attttccttg gctgaatgta cagcatgagc aatcgtttca tcacacagat cataaccggt
     1081 gaattggctt tgaggatagg ttttagccat taatatcaag gaacgcccat aaccacaacc
     1141 aatatcgagt acctgaattc cctggttcag tttgtcaacc aagccgtgaa ccataggtaa
     1201 aattttgtca gtcaaggaag ataaaatcgt ttgtccactc tcttccgcca tgacttcatg
     1261 aaaacgatga taagcctcat aaggaacacc gccgccgtgt ttaaaagcat ccactacgcc
     1321 attttccaca taggccaaaa ctggaataaa ctgcgctacc actgccatat tctgtggcga
     1381 agatttcctc gtaagaaaag aagcatgttc agcgggcaat tgataggctt ttgcatgcgc
     1441 atcataatcc acaatgcctc ccaccacgag agcattgagc cattccctga cataacgttc
     1501 attacaacct gccgcctcgg ctatcacttc actgcttgca aaatcaagct cgtccatgac
     1561 atcaaacaag ccggtacgat gcccaatgga tatcatcaaa gttaatgcgg catcattgag
     1621 aacagacaaa agacgctcag caaacgcatc agctcgggct tgatcaaaac gtcctgtttt
     1681 catcttgcgc tccccaaaaa ggtaatcttc agaatcccat agagaaaata atgtatctaa
     1741 cgtagtagat aaggattata aaagaagcag tcgctatcca gaggtcgtat gggctgagga
     1801 gatgcgta
//
LOCUS       DASLOE010000290         1807 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.292, whole genome shotgun sequence.
ACCESSION   DASLOE010000290 DASLOE010000000
VERSION     DASLOE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.292"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1504
                     /gene="ceg32"
                     /locus_tag="U6309_002951"
     CDS             <1..1504
                     /gene="ceg32"
                     /locus_tag="U6309_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg32/SidI"
                     /protein_id="HEN5506353.1"
                     /translation="DEDTHLDIHIRPPDCGVFITPEDIKKFQEAGIKVNITIHEYKQN
                     YTRRYLQQYTHDLMRQANSVQFFNAEDRENAIIAATYGDCDKRNTTEPTGVAKKIREV
                     GEDFDLDKYPVQKYDLKGKSGLTVASQKLSTEPDHPLDVVAKAPNILSFGTIRPGKGF
                     EEALKLAQLIKDNSLSIHEKIKRVPIVKLAGDPQDKALMKQIVVERFGKTAVKTYQKT
                     HPYDNRFNNSQRRDYWKNLVRELNAKVKEEVAVLNNPYIEIYPWCEPHELLDLKQNCK
                     YVCRMDDMGMRNNGSAIISVLDVGVVYTKFGSVTDDIFIKGGKYGNAVDIGEYRYGKY
                     SLLKKEKEFKEQHEEEPLPKWLIKNPDSAYKRQSESRDPKDILDSIVAREENQLICDN
                     IEDSDNYRTVVEAQKLLKERFTLKNAVDHLLENIGLGHLIAQEEVDELFETVDPVQAQ
                     IDNLDILSDIKTPRLCLSRSCPELGFFGSRRGDLEAKQENNKLKESILVF"
     gene            1506..>1807
                     /gene="mesI"
                     /locus_tag="U6309_002952"
     CDS             1506..>1807
                     /gene="mesI"
                     /locus_tag="U6309_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948207.1"
                     /note="regulates activity of cognate effector SidI;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS metaeffector MesI"
                     /protein_id="HEN5506354.1"
                     /translation="MIKGKLMPNSLKDVQKLICDEGITDNVITTLSRLKPFDLAMLKA
                     TSDNKVKTLLDSDELKPFWVNKFNKLRLEKDHIFQFRNPDPQSRADFYCGYVLYLAA"
BASE COUNT          656 a          289 c          352 g          510 t
ORIGIN      
        1 tgatgaggat actcatcttg atattcatat tcgtccacca gattgcgggg tctttattac
       61 tccagaagac atcaaaaaat ttcaagaggc aggtattaaa gtcaatatca caatccatga
      121 atataaacag aactacactc gacgttattt acaacaatat actcatgatt taatgcgcca
      181 ggcaaacagt gtgcaattct ttaatgcaga agatagagaa aatgctatta ttgccgctac
      241 ttatggtgat tgtgataagc gcaatacaac tgaaccgact ggtgttgcaa aaaaaataag
      301 agaagtcggg gaagattttg acttagataa atacccggtt caaaaatatg atttaaaagg
      361 aaaatcagga ttaaccgttg catcacaaaa attaagtact gaaccggatc atccattgga
      421 tgtagtagcc aaggccccta atatcttatc ttttggtacg attcgtcctg gcaaagggtt
      481 tgaagaagcg ttaaagctgg ctcaattgat caaagataac tccctttcta ttcatgaaaa
      541 aataaaacgg gtacctattg taaaactggc aggtgaccca caagacaaag ctttaatgaa
      601 acaaatagtt gttgaaagat ttggtaaaac agcggtcaaa acttatcaaa aaactcatcc
      661 ctatgataat cgattcaata atagtcagcg cagagattat tggaaaaatt tggttaggga
      721 gttaaatgca aaagttaaag aagaagtcgc tgttcttaat aatccttaca tcgaaatata
      781 tccttggtgt gagccgcatg aattgctgga tttaaagcaa aactgtaaat acgtttgtcg
      841 aatggatgac atgggcatga gaaacaatgg ctcagcaatt atcagtgtat tagacgttgg
      901 agtcgtttat actaaatttg gttctgttac agacgatatt tttattaaag gcggcaaata
      961 cggcaatgcc gttgatattg gagaatatcg ttatgggaaa tacagtctgt taaaaaagga
     1021 aaaggaattt aaagaacagc atgaggaaga accactacct aagtggctaa tcaaaaatcc
     1081 agactctgct tataagcgac aatcagaatc gagagatcca aaggatattt tagactccat
     1141 tgttgcaaga gaagaaaatc aattaatatg tgataatata gaagattccg ataattatcg
     1201 gacagtagtc gaagcacaaa agttattaaa agagcgcttt acactcaaaa atgctgtaga
     1261 tcatttatta gagaatatag gactcggcca tttaattgct caagaagagg tcgatgaatt
     1321 atttgagaca gttgatccag tgcaagcaca aatagataat ttagacattc tttctgatat
     1381 taaaacacca cgtctgtgtt tatcacgctc ttgccctgag ctaggttttt tcggctcacg
     1441 tcgtggtgac ttggaggcta aacaagaaaa taataaactt aaagaatcga tactggtatt
     1501 ttgatatgat aaaaggaaaa cttatgccca actctctgaa ggatgtgcaa aaattaattt
     1561 gtgatgaagg catcacagat aatgtaatta cgactctatc caggttaaaa ccatttgatt
     1621 tagccatgct caaagccact agtgacaaca aagttaaaac gctactggat agtgatgaat
     1681 taaaaccatt ctgggtaaat aaatttaata aattgcgatt agaaaaagat catatttttc
     1741 aatttagaaa cccggatccg caatcaagag ctgatttcta ttgtggatat gttttatatt
     1801 tagcggc
//
LOCUS       DASLOE010000291         1766 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.293, whole genome shotgun sequence.
ACCESSION   DASLOE010000291 DASLOE010000000
VERSION     DASLOE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.293"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            397..1746
                     /gene="dsbD"
                     /locus_tag="U6309_002953"
     CDS             397..1746
                     /gene="dsbD"
                     /locus_tag="U6309_002953"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEN5506355.1"
                     /translation="MRKLIDSFMILLLIFFTQAAWSYTPGFESSNPDTVMHFITGNSA
                     FVYLSAFFGLGILLAFTPCVLPMVPILSGIIVGQNSISTGKAFKLSLSYVTGMAITYA
                     GAGMLAGYMGSTIQTVMQRPVVITLFSVVFVAMALSMFGFFELKFPTSLGNRLNKNNQ
                     LGKQRSYLSVGIMGVLSTLIVSPCVTAPLIGVLSYIGQNGQVIMGGLILFVMALGMGV
                     PLLIVGAGYGTLLPKAGSWMLKIKQLFGFIMLGIAIWMLSRILNQTITHILWAGLLMV
                     ASISLGALKSQNNWRGYLAQGLGILALISGGIIFYNTAIPSVKIKQASVENSPFIKIN
                     TLNALQLQLEQAKKVRKAVFLDFSADWCGDCQEMDTKVFNQPEIQKAMSDWVSIKVDI
                     SNKNAEVDSIKKAFGIYGTPTMIFFNTSGEPITQMTSVGLISLSKMLQLLEQARHLS"
BASE COUNT          495 a          297 c          363 g          611 t
ORIGIN      
        1 ttttgttaat gataaaacca gcagtgtttc ataacaataa tcagcagact cagagtaata
       61 cccaatatat acattctata acggaatgcc ctcagaggac ggcattgatg aattattgct
      121 cgtccagtgc ctattatatt atttctgaag aggagttatc tctcggtaat gtcttatcct
      181 tgttgacatt tatcctttat tttttagtta ttcaatcaaa atacaaatcg cctgtatccc
      241 caatttttaa accaccgatt gcccactaga gtaaatttgt caactttctc agtaggcttc
      301 ttgccagccc tggctatgca agaggtccag tgtaggttac ttgtactctg gtacaagtat
      361 ccaccgttgt tatttattac ttattgggac atcaaaatga gaaaattaat tgattcgttc
      421 atgattttat tactgatatt ttttactcag gcggcgtggt catatactcc tggctttgaa
      481 agttctaacc cggatactgt gatgcatttt attacaggaa atagtgcttt tgtttatttg
      541 agtgcttttt tcggattagg tattttgttg gcttttacgc cctgtgttct accgatggtt
      601 cctattttat caggaattat tgtaggacaa aattctattt ctacaggaaa agcctttaaa
      661 ttatcactga gttatgtgac tggaatggct atcacctatg caggagcagg gatgcttgcc
      721 gggtatatgg gaagtacaat ccagactgta atgcaacgtc cagtagtaat cactttattc
      781 agtgttgtgt ttgtagccat ggctctttca atgtttggct tttttgaatt gaagtttcct
      841 acaagccttg ggaatcggtt aaataaaaac aatcaattag ggaagcagag aagttatctt
      901 tctgttggaa taatgggagt tttatccact ttaattgttt ctccttgtgt taccgctcct
      961 ttaattggtg ttttaagtta tattggtcaa aatggccagg tcataatggg cggattgatt
     1021 ttatttgtta tggcattggg gatgggggtt cctctgctga ttgttggtgc gggttatggt
     1081 actttactcc caaaagcagg ttcctggatg cttaaaataa aacaattgtt tggttttatc
     1141 atgttgggaa ttgccatatg gatgttatcc cggattctga atcagaccat tacgcacatt
     1201 ttgtgggcag gattactcat ggtcgccagc atcagtttag gtgctttaaa atcacaaaac
     1261 aattggcgtg gttatttggc tcagggattg ggtattcttg ctttgatttc aggaggtatt
     1321 attttttaca acaccgcaat accttcagta aaaataaaac aagcgtctgt agaaaatagc
     1381 ccatttatca agattaatac cttaaacgct cttcaactgc aattggagca ggctaaaaaa
     1441 gttagaaaag cagtatttct tgatttctca gcggattggt gcggtgactg tcaggaaatg
     1501 gataccaaag tattcaatca accagaaatt caaaaagcta tgtctgattg ggttagcatt
     1561 aaagtggata tcagtaataa gaatgcagag gttgattcta taaaaaaagc ttttggtatt
     1621 tatggaacgc caacgatgat attttttaat acatcgggag agcctattac tcaaatgact
     1681 tcggttggtt tgatttcgct atcaaaaatg cttcaattgc tcgagcaagc gcgccatttg
     1741 agttgaaata atagacaatt tgctac
//
LOCUS       DASLOE010000292         1754 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.294, whole genome shotgun sequence.
ACCESSION   DASLOE010000292 DASLOE010000000
VERSION     DASLOE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.294"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..825)
                     /locus_tag="U6309_002954"
     CDS             complement(<1..825)
                     /locus_tag="U6309_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN5506356.1"
                     /translation="MQRLKNIFTAIYQYLLKETEDASHQITLFGIVMMINYPLFGVFW
                     KLEHFQLTEEFILRITAALLCACLAFNQFWPRQLLKFLPVFWYIVLLFCLPYFFAYLT
                     LINNGSTLWLMNCVSAIFFLLLVSSVLGALILLISGVGLAFFHFYILSNNQFVYIPGT
                     ISLFSLIVTFIAAIIIGALFARDREITYAGRLSGMRMLAGSIAHDLRTPLASIYLQAE
                     LQELIVERLNNPEVQKDLKENLSKITRGIEMSNQLIRMQLNNIQRDKLDTSTFSIYS"
     gene            complement(1222..>1754)
                     /gene="mesI"
                     /locus_tag="U6309_002955"
     CDS             complement(1222..>1754)
                     /gene="mesI"
                     /locus_tag="U6309_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948207.1"
                     /note="regulates activity of cognate effector SidI;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dot/Icm T4SS metaeffector MesI"
                     /protein_id="HEN5506357.1"
                     /translation="FTTFNCFYAAQEILTFLIGACKNDTKRENIDLLYNFVTSQSTQI
                     QEHKTPGCLLLANAYFYLAGFYLSLDLKAESIECYKECWGQLHLAQLLETDSEREIHN
                     AYFNKGLATSNAFGLNSISEIKARCLDLASEALPYPARNVMEANAVKTFENRFKDSMS
                     TAGKDDERSITRPIIL"
BASE COUNT          597 a          294 c          299 g          564 t
ORIGIN      
        1 ggaataaatc gaaaatgtac tggtatctaa tttgtctcgt tgaatattat ttaattgcat
       61 acgaattaat tgattactca tttctatgcc gcgagtaatt ttgcttaaat tttccttcag
      121 gtccttttga acttctggat tatttaatct ttcgacaatc aattcctgta attcggcttg
      181 taaataaata ctggcaagcg gcgttcttaa atcatgagcg atacttccag ccagcatgcg
      241 catacccgat aaacgtccgg cataggtaat ttctctatct cgtgcaaaca aggcaccaat
      301 gattattgct gcaataaacg taacaattaa actaaaaagt gatatggttc ctgggatata
      361 gacaaactga ttatttgata aaatataaaa atgaaaaaaa gcaagtccaa cacctgatat
      421 aagtaatata agagccccca atacactgga aactaataaa agaaaaaaga tagcggatac
      481 gcaattcatt agccaaagag tcgagccatt gtttatgaga gtcaaataag caaaaaagta
      541 aggcaaacaa aagagaagaa ctatatacca aaaaacaggt agaaatttta ataattgtct
      601 tggccaaaat tgattaaatg ccaaacaggc acagaggagg gctgcagtga tgcgtaaaat
      661 aaattcttct gtcagttgaa aatgctccag tttccagaaa actccgaaaa gagggtaatt
      721 aatcatcatg acgatgccaa ataaagtgat ttggtggctt gcatcttcag tttcttttaa
      781 taaatattgg taaattgcag taaatatatt ttttaacctt tgcatgattc cttaattatc
      841 tgagtaaaaa ctttgagatt gtttgggtat taatcttatt agtcctgaac attgaatagt
      901 tagtgtagga ttagaaatac ttgtataaat catgagtatt tcaatggtga actctgaatg
      961 gatgctacca attagagtta tgatttctac ggtttcttgg acttaagtta aataatattg
     1021 ctcccaatta aaacaaatac caaggatacc ataatccctg ttttttaata ctaatattac
     1081 ttttccataa gtaaaaactc atattattgc gctttaattt aatatgaatt gtcctatgca
     1141 aaatacagcg acctggaagc taatgttggt tgtttttagc atcataatac aataccataa
     1201 agagaatatc agcggactat attataaaat aattggtcga gttatactgc gctcatcgtc
     1261 tttaccagcc gtactcatgc tgtctttaaa tctattttca aaggttttta cagcgtttgc
     1321 ttccatcaca tttcgagccg gataaggtaa tgcttctgag gctaagtcaa ggcatcgagc
     1381 tttaatttct gatatggaat tcaaaccaaa tgcattactg gtcgctaatc ccttattaaa
     1441 gtatgcgttg tgaatttctc gttcagaatc tgtctctaat aattgtgcca aatgaagttg
     1501 tccccagcat tctttataac attcaatgct ttctgcttta agatccaggc tcagataaaa
     1561 tccagcaaga taaaaatacg cattagcaag caataagcac cctggcgttt tgtgttcttg
     1621 tatttgagtg ctttggctag ttacaaaatt gtataaaagg tcgatatttt ctcttttggt
     1681 gtcattctta caagcaccaa ttaagaacgt caaaatttcc tgagccgcat aaaagcaatt
     1741 aaatgttgtg aagg
//
LOCUS       DASLOE010000293         1700 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.295, whole genome shotgun sequence.
ACCESSION   DASLOE010000293 DASLOE010000000
VERSION     DASLOE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.295"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1572)
                     /locus_tag="U6309_002956"
     CDS             complement(<1..1572)
                     /locus_tag="U6309_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEN5506358.1"
                     /translation="MLAESVIGIVRAVNGLLEKVNVQGQASLVKSGARLEEGDVLTLL
                     SGEAYIQFINGFPEALALEKPVKLDGVSPTLQYGAEDLKEQMVQEAIAKGIDPSVILD
                     VLGSAAAGAEAVGSGGDAFIMDPLFGFGHVTAGYPTGPISFAYEADTQQLFWFVPEET
                     GAIAESELTQEPESIPQIPQFTTNQAVLTVFEDALPSGIPDSAGQARIASSSLSTLLT
                     SSPDVVASFAFNTNLSVLPTLKSGGIDLDYNLSSDKRTLTASIPGGAAVMQFELTADG
                     QLTQTLMDSIDHPTADSDDSEWMRLDLSPLIDVTFTRTSDGSVLESRTLPANAVVAGI
                     QDDVPIARAQLTNNEILLDETIGMKAGDADAANDDFNPTTTVDPFNNTYGIPIGLVQN
                     ANLLDTSTSEMGGDYKNATMTHLLKITDAVSGLQTTDGTPINLFLESNGDISGRAGDI
                     GAPAVFAIRMNPNTGAITVAQYGSIKQFDTNSHDEAVDLTGRISAVVRVTDGDGDVSN
                     AEIPIGQRIVFEDDGP"
BASE COUNT          487 a          375 c          319 g          519 t
ORIGIN      
        1 aggcccgtcg tcttcaaaaa caatccgttg tcctataggt atttctgcat tagatacgtc
       61 accatcacca tctgttactc tcactacagc tgaaatacgt cctgttaaat caaccgcttc
      121 atcatggctg ttggtatcaa attgtttaat tgaaccatac tgcgcaacgg taattgcacc
      181 agtatttggg ttcatgcgaa ttgcaaaaac tgcaggagca ccaatgtcac ctgctcttcc
      241 gctaatatca ccattcgatt ccaaaaaaag attgatagga gtgccatcgg ttgtctgtaa
      301 tcctgaaaca gcatcagtaa tttttagtaa atgggtcatt gttgcatttt tataatcccc
      361 ccccatttca gaagtactgg tatcaaggag gtttgcattt tggaccagac ctatgggtat
      421 accataagta ttattaaaag gatccacagt agtcgttgga ttaaaatcat cattagctgc
      481 atcagcatca ccggctttca ttcctatagt ttcatccaac aggatttcat tattggtcaa
      541 ttgagctctt gctattggaa catcgtcttg aattcctgca acaactgcat tagcaggcag
      601 tgttcttgac tctaaaacac tgccatcact tgttcttgtg aaagtcacat caattaaagg
      661 tgacaggtct aatctcatcc attcgctgtc atcgctatca gctgttgggt gatcaatcga
      721 gtccattaat gtttgagtta attgtccatc tgctgtcaac tcaaattgca tgaccgctgc
      781 cccgccaggg attgatgctg ttaaggtacg tttatcagag gatagattat aatcaaggtc
      841 tataccacct gactttaatg taggaagaac acttaaattg gtattaaaag caaaggaagc
      901 aacaacgtct ggtgaagagg tcagcaaagt acttagagaa gaacttgcga ttcttgcttg
      961 ccctgctgaa tcaggaatac cacttggcaa agcatcctca aaaactgtta gcactgcttg
     1021 atttgtggtg aattgaggaa tttgcgggat agattcaggc tcttgagtca attcggattc
     1081 tgcaatcgcc cctgtctctt ctggcacaaa ccaaaataat tgctgagtat ctgcctcata
     1141 agcaaaggaa ataggtccgg tgggatatcc tgctgtgaca tgtccgaagc caaacaaagg
     1201 atccataata aatgcatcgc ctcctgatcc caccgcctcg gcacctgctg ctgctgagcc
     1261 aagaacatca agtatgactg aaggatcaat acctttggct atcgcttctt gcaccatttg
     1321 ttcttttaaa tcttccgcac catactgtaa tgttggggaa acaccatcta gcttgaccgg
     1381 tttttctaag gctaatgctt caggaaatcc attaataaat tgtatgtaag cctcaccact
     1441 taataaagtc aggacgtccc cttcctctaa tcgagcgcca gactttacta agcttgcttg
     1501 tccttgcaca tttacttttt ctagtagtcc attgacggct cttactatac cgataacaga
     1561 ttcagctaac atgttctgtc ctcaaagttt actattattc atattaataa tagttcaagg
     1621 attaagcctc cttgtagtta aaatgtaaca aatgtgtaaa catttgaatc atatagatac
     1681 aattatgact tgtttatatg
//
LOCUS       DASLOE010000294         1692 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.296, whole genome shotgun sequence.
ACCESSION   DASLOE010000294 DASLOE010000000
VERSION     DASLOE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.296"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>1692
                     /locus_tag="U6309_002957"
     CDS             <1..>1692
                     /locus_tag="U6309_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEN5506359.1"
                     /translation="YKFGKKEENISFSLINTPCAKVIQKKAICYFNDVQKQFPNDKLL
                     KKMKINTYLAGPITNPQDEVIGIMTILCKREVEFDTLNKTLFGLFLSRINSEIERLIN
                     LRKLEFLASIPQQNPNPIIRILLNGDVVFTNDEGKIILNYWIKLFNGLPVELLKEAQR
                     IQKSNQSTRIEMEIEDKIYLFTLVWIDEFKQINIYGTDITPLKNTQNAMLNLTRRDTL
                     TQIANRQYFEEKLIEKIYEHHLKEKSLALLLIDLDNFKIVNDTLGHPTGDKLLMAVTN
                     RMTRCLRANDFIARLGGDEFIVLLDQCNTDTAVKVAEKIINVLGKPFQFDEYHMTITA
                     SIGIAIYPETSEIASDLLKYADIAMYQSKKTGKNKCTVFSKNLHYKENQRNEILKKDI
                     ELAALKRELYIDYQPQIDLSDNTIVGIEALLRWSHPTLGLILPTEFIPIAEQTGCIHT
                     ISQWFIEQTLLDYSQFQQLSSNIKLSINVSLSQLNDTRFTKILCDSLLQNNLSKSKII
                     LDISERMIAPHFIQISKKLKQIHHIGINISLDNFCCPQISLPELLSLPLDYLKVDQ"
BASE COUNT          647 a          305 c          267 g          473 t
ORIGIN      
        1 tataaatttg gaaagaaaga agagaacatt agtttttctt taatcaatac accctgcgct
       61 aaagtaatcc agaaaaaagc tatctgttat tttaatgacg ttcaaaagca atttccaaac
      121 gataaattat tgaaaaagat gaaaatcaat acatatctcg caggcccaat tacaaatccc
      181 caggatgagg tgattggcat aatgactatc ctgtgtaaac gcgaagtcga atttgacaca
      241 ctgaataaaa ccttgtttgg tttattttta agccgaatca attcggagat tgagagactg
      301 ataaacctta gaaaattaga gtttctggcg agtatacccc agcaaaaccc taatccaatc
      361 ataagaattc ttctcaatgg ggatgtcgta ttcactaacg atgaagggaa aatcatttta
      421 aactactgga taaaattatt taatggactt ccagtagagt tactaaaaga agcacaaaga
      481 attcagaaat ccaatcaaag cacaagaatt gaaatggaaa ttgaggataa aatctactta
      541 ttcacacttg tctggattga cgaatttaaa caaatcaata tttacgggac tgatataacc
      601 ccgctcaaga atacgcaaaa cgctatgtta aatctaaccc gcagagatac tttaactcaa
      661 atcgcaaatc gacaatattt tgaagaaaaa ctaattgaaa aaatatatga gcatcatctg
      721 aaagaaaaaa gcctggcatt attattaatc gatttagata actttaaaat tgttaatgat
      781 acattagggc atccaacagg agacaaacta ctgatggctg ttaccaatag aatgacccga
      841 tgcctaagag caaatgattt tattgcccgc cttggaggag atgagttcat cgtccttctt
      901 gaccaatgca ataccgatac cgcagttaag gttgcggaaa agattattaa cgtgctcggc
      961 aaacctttcc aatttgatga atatcatatg acaatcacag ccagtattgg cattgccatc
     1021 tatcctgaaa caagtgaaat agccagcgac ttactaaagt atgccgatat tgcaatgtac
     1081 caatccaaaa aaacaggtaa aaacaagtgc actgtgtttt caaaaaactt acactataag
     1141 gaaaatcaaa gaaatgaaat tctcaaaaaa gacatagaat tagcagcatt aaaaagagaa
     1201 ctctatatag actaccaacc ccaaattgac ttatccgata acacaatagt tggaattgaa
     1261 gctttattac gatggtcaca ccctactctg ggtttaatat tacctactga gtttattcct
     1321 atagcagagc aaacagggtg tatacatacc atcagtcaat ggttcataga acagacatta
     1381 cttgattact cccagtttca gcaattaagt tcaaacatca aattgtcgat aaatgtttcc
     1441 ttaagtcaat taaatgacac gcgatttact aaaatattgt gtgatagttt actgcaaaat
     1501 aatttaagca agagcaagat cattttagat atatcagaac gaatgattgc cccacacttt
     1561 attcaaatat caaaaaaatt aaaacaaata catcatattg gaattaatat aagcttggat
     1621 aatttttgct gcccacaaat ttcccttcct gaattgctgt cattaccact tgattatctc
     1681 aaagtagatc aa
//
LOCUS       DASLOE010000295         1684 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.297, whole genome shotgun sequence.
ACCESSION   DASLOE010000295 DASLOE010000000
VERSION     DASLOE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.297"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            48..569
                     /locus_tag="U6309_002958"
     CDS             48..569
                     /locus_tag="U6309_002958"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN5506360.1"
                     /translation="MRQVTKYTEAIKASVLSKALAPNAPGVIELAKEFNIPKATIYTW
                     LINMKNKNNKKEQTRQRPKDRSPAFKLQAVMDTLGKTEEEQSAYCRSHGIYYDHIEAW
                     RQQILESLGAVPNTTDKAKEKENKAENQRIQDELKALKSNLKRKDKALAELTALLVLK
                     KKADLLWGENEGD"
     gene            545..1603
                     /locus_tag="U6309_002959"
     CDS             545..1603
                     /locus_tag="U6309_002959"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN5506361.1"
                     /translation="MGGKRGRLISEQDKMDAIGLIKEACAAGSRLKPACELLEFDART
                     FQRWNKEGAVQDKRRGPLTAPRNKLSDTERARILAVVNLPEYRNQPPSQIVPNLADKG
                     EYIGSESTIYRVLHAHKMMQHRSAARPRTHRKPDELTATKPNQLWSWDITYLTSNIRG
                     NYFYLYLFLDVFSRKIVGFNVFDEQSAEHASEVVSAAYITENISAGEVTLHADNGGPM
                     KGSMMLATLQNLGIASSFSRPSVSNDNPYSESMFRTLKYCPQYPNKPFESTEAALEWV
                     RKFVDWYNNVHQHSGNNFVTPNARHKEQDRAILAKRTQVYERAKQENPSRWSGKTRNW
                     ARVEEVLLNTKRTTRKAA"
BASE COUNT          572 a          329 c          374 g          409 t
ORIGIN      
        1 tcgcctctga attaaattaa ttctgtgagg cggaactgga gttcctgatg aggcaagtca
       61 caaaatatac agaggcaata aaagcaagtg tattatccaa agcattagca cctaatgcac
      121 caggtgtaat agaattggcc aaggaattta atatacccaa ggctaccatt tacacatggc
      181 ttattaatat gaaaaataaa aataataaga aagagcagac tcgccagcgc cctaaggatc
      241 gatcgcctgc atttaaatta caagcggtta tggataccct tggaaaaaca gaagaagaac
      301 aaagtgctta ctgtaggtca catggcattt attatgatca tatagaagca tggaggcagc
      361 agattttgga aagtcttggt gctgtgccta acactacgga taaagccaaa gaaaaagaaa
      421 ataaggctga aaatcagcgt atccaagatg agttgaaggc gttaaaaagt aatttaaagc
      481 gtaaagataa ggccctggct gaattaactg cactattagt tctcaaaaaa aaagcagatt
      541 tgctttgggg ggaaaacgag ggcgattaat aagtgaacaa gataaaatgg atgcgattgg
      601 attgataaaa gaagcctgcg ctgctggaag caggctaaaa cctgcctgcg agcttcttga
      661 atttgatgcg cgaacctttc agcgatggaa taaagaaggc gctgttcaag ataaacgccg
      721 tggcccactg acagcacccc gcaacaaact cagcgataca gagcgcgcac ggatactcgc
      781 agtagtcaat ttgcctgaat accgtaatca gccaccaagt caaattgtac ctaatcttgc
      841 tgataaaggt gagtatattg ggtcggaatc aacgatatat cgcgtgcttc atgcgcataa
      901 gatgatgcag caccggagcg ctgcgcgacc caggacgcat cgaaaaccag atgagctcac
      961 tgccaccaaa cccaaccagc tatggtcttg ggatataacc tacttgacca gcaacatacg
     1021 cggtaactat ttttatcttt atcttttttt agatgtgttc agtagaaaaa ttgttggttt
     1081 taatgtgttt gatgagcaat ctgctgaaca tgcctcagaa gtggtctctg ccgcgtatat
     1141 aacagaaaat ataagcgctg gcgaagtgac gctacatgca gacaatggcg gcccgatgaa
     1201 aggttcaatg atgctggcaa ccttgcaaaa tcttggcatc gcttcgtctt ttagtaggcc
     1261 atctgtcagt aatgataatc cctattcaga atcaatgttt agaacactaa aatattgtcc
     1321 tcaatatcct aataaaccat tcgagtccac ggaagcagca ttagaatggg taaggaaatt
     1381 tgttgattgg tataataacg tccatcagca tagcggcaat aatttcgtca cacccaatgc
     1441 gcgacataaa gaacaagatc gagcaatctt ggcaaaaaga acacaagtat atgaacgagc
     1501 caaacaggaa aatccgagca gatggtctgg aaagacgcgt aattgggctc gtgtagagga
     1561 agtattatta aacactaaac gaactacccg caaagcggca tgatgacatt aaaaaaaata
     1621 ttgcgttatt tttttattga gatattatgc tccgagtaga gcaaatctgt tctgcttcaa
     1681 gcga
//
LOCUS       DASLOE010000296         1682 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.298, whole genome shotgun sequence.
ACCESSION   DASLOE010000296 DASLOE010000000
VERSION     DASLOE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.298"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            476..1186
                     /locus_tag="U6309_002960"
     CDS             476..1186
                     /locus_tag="U6309_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506362.1"
                     /translation="MRNLKLSHQYYIGSSLFLFILLFVPFLSYGAETSNMRGKRYCEI
                     ILTKTISSYAVYNTWGLNDCPEQLWSKVSISAVKKETGSSFVHLNGPRYWVIDGFKNT
                     SLINPAIKTISGIPMREAGILHLSLMDLFKNKPYQSHVVDRKTTWVYQADKPVFELID
                     PNGQVFVMQSYSVQKYPQTMNTLTQLGAKLQLPKGWKFKTGVLNKPETIQAVNNKAVV
                     VQDNFLNTYQKASHDFLK"
     gene            1402..>1682
                     /locus_tag="U6309_002961"
     CDS             1402..>1682
                     /locus_tag="U6309_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506363.1"
                     /translation="MILRLILAFYLIIFPHKFLMANQVMNTAIKVLEECYNKATDLRN
                     YVPCVETEAEKIHTLQNLQIRIKFKNPEKNSKEKVPILMIDKTGYMYYC"
BASE COUNT          598 a          298 c          271 g          515 t
ORIGIN      
        1 ggcaaaaaga acaagcggcc aaacaactgg taaaccttga tagtttaaga attaatttct
       61 tgaaagagtt aatgcatttc atttgtaatt caaataccat ttgaattttt acataatgga
      121 cttcaatcaa aaagcctgat tacatccaag agaaagctca aaattttctt aatttcaact
      181 ttagccactt tttcgtgaac tgctcatcct gaggagcgtg cgcagcactc gtctcgaagg
      241 actagttaag cagccaaaaa gagataataa atcaaaatat taattatttt ctctgggata
      301 gacagcttac gaaaaagtgg ctaaagtcga gttaatgatc cctcaattcc aactccaaaa
      361 atagccattg aagcttattg aactacagcc tatttgggca tttcccaatt ttgtagacag
      421 aatagccttt ataaattaaa gtccaagtag atataaaaat gacaaggaga tagtcatgcg
      481 caatttaaaa ctttcgcatc agtattacat aggaagctct ttattcttat ttattttact
      541 ctttgttccc ttcctatctt atggtgcaga aacatccaac atgcgtggca agcgatattg
      601 cgaaatcatc ttgacaaaaa ccatatccag ttatgctgtt tataatactt ggggattaaa
      661 tgattgcccg gagcaattat ggagtaaagt ttctatttcc gcagtaaaaa aagagacggg
      721 ctcttctttt gttcatctca atggccctcg atattgggta attgacggat tcaaaaacac
      781 ttctttgata aatccagcca tcaaaacaat tagtggaatt ccaatgcgtg aagcaggcat
      841 tttgcacctt agtttaatgg atttatttaa aaacaaaccc tatcaatcac atgtcgtgga
      901 ccgcaaaaca acatgggtct atcaagcaga taaacctgtc tttgaattga ttgatcccaa
      961 cggacaagtt tttgtaatgc aatcctatag tgttcaaaaa tacccacaaa caatgaatac
     1021 cctcacacaa ctaggcgcca aacttcagct tcccaaaggt tggaaattta aaactggtgt
     1081 gcttaacaag ccagaaacaa ttcaagctgt taataataaa gccgtagttg tccaggataa
     1141 ttttttgaac acctatcaaa aagccagcca tgatttcctg aaataaattc agttgataat
     1201 tggttacttt cataggtcat gagtttatta tgcgttgaca atccagcctc tactttgggg
     1261 ctaatggcat taatgaacgt gttaatggta atttcaagca tctataaaac ctatgattag
     1321 caaaccaagg taaaataaca aacattttat aaatgttgta ttataattaa cctatacgga
     1381 gtatcttaga ggttaattat aatgatatta agattaatcc ttgcttttta tttgattatt
     1441 ttcccccata aatttttaat ggctaatcaa gttatgaata ctgctattaa ggtattggag
     1501 gaatgttata acaaagccac tgatcttaga aactatgtcc catgtgttga aacagaagca
     1561 gagaaaatac acaccttaca aaatttgcaa atacgcataa aatttaaaaa tcctgaaaaa
     1621 aattctaaag aaaaagtacc catattgatg atagataaaa caggatacat gtattattgt
     1681 at
//
LOCUS       DASLOE010000297         1677 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.299, whole genome shotgun sequence.
ACCESSION   DASLOE010000297 DASLOE010000000
VERSION     DASLOE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.299"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..309
                     /locus_tag="U6309_002962"
     CDS             <1..309
                     /locus_tag="U6309_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEN5506364.1"
                     /translation="MQWNFASMGIDVVMSGHDHHYERIERNGIVYFVNGVGGAELYSY
                     KKWVEGSKFFYSKHHGFMLITALEHAMKIQFINENDEIKDEIVIQEKKQGAGEHRNLR
                     "
     gene            623..>1677
                     /locus_tag="U6309_002963"
     CDS             623..>1677
                     /locus_tag="U6309_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN5506365.1"
                     /translation="MKKSTRTTPLVSKGISKTEPIPKHLEQINQWAAGIDIGATSHFV
                     AVPEGCTTTPVREFKSFTPDLYALAKWLKECGIQTIAMESTGVYWIPVYELLEEKGFE
                     VKLVDARRVKNVSGRKTDVLDCQWIQQLHTYGLLNGAFRPDNEICALRSYVRQRSMLI
                     RSASRYIQQMQKALTQMNLQLHHVITDITGDTGMRIIRAIINGEREPALLANHRDPRC
                     KSSKEDIEASLTGNYREEHLFTLRQSLELYDYFQEKIQACDSEIEKSLSGFSTNTLGK
                     DFSVEKLKFNKKKVSKNAPAFNLRKELYRLAGVDLLAVPGINNISALQLLSEIGFDMT
                     RWKNSKQFSSWLGLCPG"
BASE COUNT          573 a          275 c          357 g          472 t
ORIGIN      
        1 atgcaatgga attttgcctc tatgggtatt gatgtggtta tgagtggtca tgatcatcat
       61 tacgagcgta tcgaacgcaa cgggattgtt tattttgtca atggagtcgg aggagctgag
      121 ctttattcct ataaaaaatg ggtagaagga agcaaatttt tttactctaa acaccacgga
      181 tttatgctga taacagcttt ggaacatgcc atgaaaatcc aattcatcaa tgaaaatgat
      241 gaaattaagg atgagatagt cattcaagaa aaaaaacagg gggctggaga acatcggaac
      301 ttacgataaa ctccagtctc tttgttaaaa aatgttttta cctcagcctt gggggttttc
      361 gtaagcattt aaagcacgag tgctccacat ctataaataa ctttaaatat tttatgtaga
      421 atctcacacg atgggatcat gaaaaagaag gccgattgga cattggtgtc attgtacccg
      481 ctcgtaagca tggtcccgga ggattaaagg tagcatcata ggtgaggtct gacataccat
      541 cccgtttaga agagtaccga aataaaaatc ctctgtttta tggacccatc attttcattt
      601 aattgactaa aaagagaatg acatgaaaaa aagtacacga actaccccgc tggtgtcaaa
      661 gggaatatct aaaacagaac cgatacccaa gcatctcgaa caaattaatc aatgggcagc
      721 tggaattgat ataggagcca catctcattt tgtagctgta cctgaaggat gtacaacaac
      781 accagttcgt gaatttaaga gttttactcc agatctttat gcattggcaa agtggttaaa
      841 agagtgtgga atacaaacca ttgcaatgga gtcgacgggg gtttattgga tcccggttta
      901 tgaattatta gaagaaaaag gatttgaagt taaactagtt gatgcaagac gagtaaaaaa
      961 tgtgagtggt cgtaaaacag atgtattaga ttgccaatgg atacaacagc tacataccta
     1021 tggtttatta aatggggcat ttcgtccgga taacgagata tgtgctcttc gctcgtacgt
     1081 tagacaacgt agcatgttaa taagaagtgc atcaaggtat attcaacaaa tgcaaaaagc
     1141 actcactcaa atgaatcttc aattgcatca tgtgattaca gatattactg gcgatacggg
     1201 gatgagaatt attcgggcaa ttatcaatgg agaacgagag cctgctcttt tagcaaacca
     1261 tagagacccc aggtgtaaaa gcagcaaaga agatattgag gcttcattaa ctggtaatta
     1321 tcgagaggaa cacttattta cactcagaca atcacttgag ttgtatgatt attttcaaga
     1381 aaagattcaa gcctgtgata gtgaaataga aaaaagctta tctggatttt caaccaatac
     1441 cttagggaag gatttttctg tcgagaagct gaagttcaat aaaaagaaag ttagtaaaaa
     1501 tgcacctgct tttaatttaa gaaaggagtt atatcgttta gcaggagtag atctattagc
     1561 tgtgcccggc ataaataaca tatcagcttt acaactatta agtgaaattg gttttgatat
     1621 gactcgatgg aaaaattcaa agcaattttc atcctggctc ggcttatgcc ctggcaa
//
LOCUS       DASLOE010000298         1668 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.300, whole genome shotgun sequence.
ACCESSION   DASLOE010000298 DASLOE010000000
VERSION     DASLOE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.300"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..219)
                     /gene="coaE"
                     /locus_tag="U6309_002964"
     CDS             complement(<1..219)
                     /gene="coaE"
                     /locus_tag="U6309_002964"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215598.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEN5506366.1"
                     /translation="MVYSVGLTGNIASGKSTVAEFFSELGINVIYADKIAKELTSKNT
                     PCYQDIISHFGSSVVLNNGELDRKRIRDI"
     gene            complement(191..664)
                     /locus_tag="U6309_002965"
     CDS             complement(191..664)
                     /locus_tag="U6309_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506367.1"
                     /translation="MRNYIFYGLLLLIGLSHQGFTAPMMKKGSYWKCVTYDKANKAWT
                     AQSSYRKVAINVAFAACKKESQLPATCKTSISNCEGFINGVSTRPMWRCTAIDITAQP
                     WESNFYSNRDDAALAAQAYCKENSTLPATCYINMVTCANKNEGAHSDGLFSGTNW"
     gene            complement(661..>1668)
                     /locus_tag="U6309_002966"
     CDS             complement(661..>1668)
                     /locus_tag="U6309_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEN5506368.1"
                     /translation="RIRYTEQNKNWVLSNTQETAPLGLVAIEKLQSINGVSVVVSENN
                     KTIRHNFWYSQTITILIVLICSFILYALIKNMMTKRYSLQGAIKLAIKNEEFYPVYQP
                     LFDCDEERFTGVEVLLRWENEDSQIIMPDFFIAEAEATGLIVPITLQIIEIAFKEFRS
                     LLEERSYFHLAFNISALHFTDSVFFNQFHKLIEKYNISPHQIIFEITERELLDKNDTT
                     FINKMQELRQAGFSLAVDDYGTGHASISYLQHFPFNYLKIDKLFVQAIGSNDIIESLN
                     DAIIQMAKGLNLVTIAEGVETKDQANYLANNGVRLQQGWYYSKGLSIAELTALLKGEK
                     I"
BASE COUNT          512 a          298 c          288 g          570 t
ORIGIN      
        1 aatatctctt atacgcttcc tatcaagctc accattatta agaaccactg aagaaccaaa
       61 atgtgaaata atatcctggt agcagggtgt atttttagaa gttaattctt tggcaatttt
      121 atctgcataa ataacattta tacccagctc ggaaaaaaat tctgcgactg tacttttgcc
      181 actggcaata ttaccagtta gtcccactga ataaaccatc tgagtgggct ccttcatttt
      241 tgtttgcaca agttaccata ttaatataac aagtagcagg tagtgtactg ttttctttac
      301 aataggcctg ggcagctaag gcggcatcat cccgatttga gtaaaaatta ctttcccaag
      361 gttgggcagt aatatcgatg gctgtgcagc gccacattgg tctggtgctt actccattta
      421 taaatccttc acaatttgat atggatgtct tacatgttgc gggaagttgg ctttcttttt
      481 tgcaagcagc aaatgcaaca ttgatagcca cctttcggta tgagctttgc gccgtccatg
      541 ctttgttggc tttatcataa gttacgcatt tccaatagct tccttttttc atcattgggg
      601 cagtaaagcc ttgatgagat aaaccaatta atagtagtaa accataaaaa atataattcc
      661 tcatattttt tctcctttaa gcaaggctgt taattcagca atggatagcc ctttggaata
      721 ataccagcct tgttgtaaac gaacaccatt atttgccagg taattagctt ggtcttttgt
      781 ttccacgcct tctgctatag taactaagtt taatcctttc gccatctgga taatggcgtc
      841 atttaatgat tcgataatat cattagaacc aattgcttga acaaataatt tatcaatttt
      901 taaataattg aatggaaaat gttgcaaata actaatgctg gcatggccag tcccataatc
      961 atcaacagca agtgaaaaac ctgcttgcct tagttcttgc attttattga taaaagtggt
     1021 gtcgttttta tcgaggagtt ctctttcagt aatttcaaag attatttgat gcggtgaaat
     1081 attatatttt tctattagct tatggaattg attgaaaaat actgaatctg tgaaatgtag
     1141 tgcagaaatg ttaaatgcaa ggtgaaagta agatcgttct tctaatagag atctaaattc
     1201 tttgaaggca atttctatga tttgaagtgt aattggcaca attaacccgg tagcttctgc
     1261 ttcagcaata aaaaaatcag gcataatgat ttgactgtct tcattttccc atcgcaataa
     1321 cacttcaacc cctgtgaatc gttcttcatc acaatcaaat aaaggttgat ataccggata
     1381 aaattcctca tttttaatag ccagttttat tgcgccttgc aatgaataac gtttggtcat
     1441 catatttttt ataagggcat acaaaataaa ggaacaaatc aaaacaataa gaatggttat
     1501 tgtttgacta taccaaaagt tatgacggat ggttttattg ttttcagaaa caacaacaga
     1561 aacaccattt atgctttgta gtttttctat agcaacgaga cctaatgggg cagtttcttg
     1621 cgtattgctt aatacccagt ttttattttg ctcagtatat cgaatacg
//
LOCUS       DASLOE010000299         1639 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.301, whole genome shotgun sequence.
ACCESSION   DASLOE010000299 DASLOE010000000
VERSION     DASLOE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.301"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(3..1571)
                     /locus_tag="U6309_002967"
     CDS             complement(3..1571)
                     /locus_tag="U6309_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISLpn13 family transposase"
                     /protein_id="HEN5506369.1"
                     /translation="MIKQENWIYPIELSKKEEKICSKLKQNGKLFIFLRKNRHLIFTA
                     DISQKLLSMYADYPRGKPLISPTLLAMVSILQAYEQLSDSSAVLETMFDQRWQMVLGC
                     LGAEESIFSQGTLCDFRHRLVKHNLDTMLLEQTVEVAKEVGGFCYKQLRIALDSAPLE
                     GAGRVEDTFNLIGHALELVVDCAAQIKQVDEIEIIKEAGTYLIGRSSIKAALDIDWSD
                     IQEKQKAINTLLKDVEALQRWLSGQSDSLINNQYLKEALSLLDTVLNQNIEPDPDGGS
                     KIIRGVSTDRRISISDSDMRHGRKSSSRTINGFKQHIAIDLDHKLILATSVRPANEPE
                     HEASQWLKPKVEKYGEVASKSIDRGYLSASWTAELFSQGKKIIAKPWNPATHGKYGKK
                     DFIIDLQNSMVICPAGKQQKITGKPGSLMAKFSAKLCGACPIKNKCSDSKTGRVIQIH
                     DQEKMLQALKYYVTSPEGRQEARERVKVEHALATICNRKGPRARYIGLRLNEYDLNRT
                     AMITNLHIAMNLAA"
BASE COUNT          464 a          353 c          269 g          553 t
ORIGIN      
        1 atttacgcag ctaaattcat agcgatgtgt aaattagtaa tcatggcagt tcggttcaaa
       61 tcatactcat taagtcttaa tccgatatat cttgctcttg gtcctttacg attacaaatg
      121 gtcgcaagag catgctctac tttaacacgt tctcttgctt cctgacgacc ctcaggcgac
      181 gtaacataat atttcaatgc ctgcagcatt ttctcttggt catgaatttg aatcactctt
      241 ccagtctttg agtcactgca tttgttcttt atgggacaag caccacagag tttggctgag
      301 aatttggcca tcaggcttcc tggtttgcct gtgatttttt gctgtttgcc agcaggacaa
      361 atgaccattg aattttgtaa atcaatgata aaatcttttt tcccatattt tccatgtgtt
      421 gctggattcc atggcttagc aatgatcttt ttaccttgtg aaaataactc agcagtccat
      481 gatgcagaaa ggtacccccg atcaatactc tttgaggcaa cctccccata tttttcaact
      541 tttggcttaa gccattggct tgcttcatgt tctggttcat tagctggacg tacgcttgtt
      601 gccaaaatta acttatgatc aagatcaata gcgatatgct gtttaaatcc attgattgta
      661 cgagagcttg atttacgacc atgacgcata tcactatctg agatagagat tcttctatct
      721 gtagaaacgc ctctgataat tttggaacca ccatctggat caggttcaat attctgattt
      781 aaaacagtat ctaataatga caatgcttct tttagatatt gattgtttat caatgaatca
      841 gattgcccgc ttagccagcg ttgtaatgct tcaacatctt taagcaatgt attaatggct
      901 ttttgttttt cttgaatatc cgaccagtca atatccaagg cagcctttat actactacgg
      961 ccaattaagt atgtgcccgc ttccttgata atctcaattt catcaacttg ctttatttga
     1021 gcagcacaat caaccactaa ctctaaggca tgaccaatca aattgaaggt atcttctact
     1081 cgaccagcgc cttctaatgg cgccgaatct agcgctattc ttaactgctt ataacaaaat
     1141 ccaccaactt ccttggctac ctctaccgtt tgctcaagca gcattgtatc caaattatgt
     1201 tttactaatc gatgcctaaa atcacacaat gtaccttgag aaaagatgct ttcttctgct
     1261 ccaagacagc ctaaaaccat ttgccagcgt tgatcaaaca tcgtttcaag aacagctgaa
     1321 gaatccgata attgctcata agcttgtaat atcgaaacca ttgcaagaag cgtagggcta
     1381 atcaatggct taccacgagg ataatctgca tacatcgaca ataatttctg gcttatatca
     1441 gcagtaaaaa taagatgtcg attttttctt aaaaaaataa ataacttccc attttgtttt
     1501 aatttgctgc agatcttctc ttcttttttt gatagctcaa tggggtaaat ccagttttcc
     1561 tgctttatca tgcacactcc tttacacttg tctgcatgaa tttgatcata aaatttgaac
     1621 aatgtcgatc tggttaaaa
//
LOCUS       DASLOE010000300         1556 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.302, whole genome shotgun sequence.
ACCESSION   DASLOE010000300 DASLOE010000000
VERSION     DASLOE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.302"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            127..1125
                     /gene="aph(9)-Ia"
                     /locus_tag="U6309_002968"
     CDS             127..1125
                     /gene="aph(9)-Ia"
                     /locus_tag="U6309_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(9)-Ia"
                     /protein_id="HEN5506370.1"
                     /translation="MLRNNIPDQHLIALLKVYYGIDIHTVQLIVGGADMNAFGYKADS
                     ESNSYFVKLKYGHHDEINLSIIRLLHDSGVKEIVFPINTLEANLFQQVDHVKIIVYPF
                     IDAPNGFTKNLTEKQWKQLGKILRQIHGTSVPTSIQQRLRKETYSPKWREIVRSFYSQ
                     LGFDERDDQITTDFKAFFNQNIDSIHQLVNSSGELSKKIQPDFEKYVLCHSDVHAGNV
                     LVVDEESIYIIDWDEPMFAPKERDLMFIGSGIGNVWNKPHEINYFYEGYGKTSIDKTI
                     LSYYRHERIVEDIAVYGHDLLSRDQNAQSRLESFKHFKSMFTPNDVVEIAFATGLI"
     gene            complement(1314..>1556)
                     /locus_tag="U6309_002969"
     CDS             complement(1314..>1556)
                     /locus_tag="U6309_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506371.1"
                     /translation="KSKQMIKSTISSLKRESGMEVFLSDLAEQILNTINTFLNNTLNS
                     SASNRSGFFGFKSSYEKVIAQELEKNIDKELKDFKP"
BASE COUNT          519 a          252 c          245 g          540 t
ORIGIN      
        1 tctcaattta ctcattaagt ctgattattt tgataatcag acttaatgtg attatagtaa
       61 taatagtatt ctaattatat tatttgcttt accattatga taatttccaa atagtaatta
      121 actcttatgc taagaaataa cattccagat caacacctca ttgcgctttt gaaagtctat
      181 tatggaattg atattcatac agtacaactc atcgtgggtg gtgctgatat gaatgccttt
      241 ggatataaag cagattcaga atccaattct tatttcgtaa agctaaaata cggccatcat
      301 gatgaaatta atttatcaat catccgtctc ttacatgact ctggagtaaa ggaaattgtt
      361 tttcctatca acacactgga agcaaactta tttcagcaag tggatcatgt taaaataatt
      421 gtgtatccat ttattgatgc gcccaatggc ttcaccaaaa atttaacaga aaaacagtgg
      481 aaacagcttg ggaaaatatt aagacaaatc catggaacct ctgttcccac ctcgattcaa
      541 cagcgattaa gaaaagaaac ttactcacct aaatggcgtg aaatcgttag gtctttttac
      601 agtcaacttg gatttgatga gcgtgatgat caaataacca ctgacttcaa agcttttttt
      661 aatcaaaata tcgattcaat ccatcaatta gttaattctt caggagaact gtctaaaaaa
      721 attcaacctg atttcgaaaa atatgtactt tgtcattcag acgtacatgc aggtaatgtg
      781 ttggtcgttg atgaggaatc tatttacatt attgattggg atgagcccat gttcgcacca
      841 aaagaacgtg atttgatgtt cattggcagt ggtattggta atgtatggaa taaacctcat
      901 gaaatcaatt atttttatga aggttatggt aaaacaagca tcgataaaac cattttgtct
      961 tattacagac atgagcgaat tgttgaagat atagccgtgt atggacatga cttgctttca
     1021 cgtgatcaaa atgctcaatc cagacttgaa agttttaaac attttaaatc gatgtttact
     1081 ccaaatgatg tagttgaaat agcttttgct acaggtttga tttgatttgg ataaacacag
     1141 aagaaaaaag atgaaccgtt taatctcaac cctcctcttt ttaatttcgt ataaactttg
     1201 aattgcatta aatcacctga aattttttat ataacgcatc ccaactttag taagaagacg
     1261 acatgactgt ttgtgttatc gcaatgataa gctcttgtaa ataattttaa attttatggt
     1321 ttaaaatctt ttaattcctt gtcgatattc ttttcaagtt cctgggctat tactttttca
     1381 taagacgact taaaaccaaa aaaaccacta cgatttgagg cacttgagtt caatgtatta
     1441 tttagaaatg tatttatagt atttagaatt tgctcagcga gatcacttaa aaaaacttcc
     1501 attcctgact ctctctttaa agatgaaatg gtacttttta tcatttgttt cgattt
//
LOCUS       DASLOE010000301         1523 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.303, whole genome shotgun sequence.
ACCESSION   DASLOE010000301 DASLOE010000000
VERSION     DASLOE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.303"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            222..458
                     /gene="rpmB"
                     /locus_tag="U6309_002970"
     CDS             222..458
                     /gene="rpmB"
                     /locus_tag="U6309_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEN5506372.1"
                     /translation="MSRVCQVTGKRPMVGHKVSHANNKTKRRFLPNIQNHKFWVEEEN
                     RFVTLRLSTKGMRIIDKLGIKAVLDKIRAKGEKI"
     gene            473..637
                     /gene="rpmG"
                     /locus_tag="U6309_002971"
     CDS             473..637
                     /gene="rpmG"
                     /locus_tag="U6309_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN5506373.1"
                     /translation="MAAVTIKVKMESTAGTGYYKTTTKNPRNHPEKMELMMYDPKVRK
                     HVLFKEKKVK"
     gene            849..>1523
                     /locus_tag="U6309_002972"
     CDS             849..>1523
                     /locus_tag="U6309_002972"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326638.1"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; sigma 54 factor is
                     responsible for the expression of enzymes involved in
                     nitrogen assimilation and metabolism; the rhizobia often
                     have 2 copies of this sigma factor; in Rhizobium etli
                     RpoN1 shown to be involved in the assimilation of several
                     nitrogen and carbon sources during free-living aerobic
                     growth and RpoN2 is involved in symbiotic nitrogen
                     fixation; in Bradyrhizobium both RpoN1 and N2 are
                     functional in free-living and symbiotic conditions, rpoN1
                     gene was regulated in response to oxygen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEN5506374.1"
                     /translation="MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESN
                     PLLEATPVEEKEDVQINTNQNQDEYSDFQWSQLYNNLNKRNNFEENDYNFDNLHCTTT
                     NLQDHLNWQLDLTPMSDIDRVIATTIIDAINDDGFLTIPVAEIHASLNSDDFPLDIEE
                     IEAVRHRLQLFDPVGCASVNLAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNY
                     RQLMKNH"
BASE COUNT          547 a          292 c          264 g          420 t
ORIGIN      
        1 atattctact tttaccagat acctgtttgg acaacagccc tgaaaaatca gtttttaatg
       61 aaaacaaagt aaaaatattg caatatttgg gtttttttgt tataaaaccg ctcttgtatt
      121 tcatatctgg ttatttttta caacgaaagc ccgccaaaat aaacaccact gtagtgcatt
      181 ggtaagggct gttaaaattt tttttatctt ggagtaacac aatgtctaga gtatgtcagg
      241 taactggcaa gcgacccatg gttggtcata aagtgtcgca cgctaataac aaaaccaaaa
      301 gacgcttcct gcctaacatt cagaatcata aattctgggt tgaagaagaa aatcgatttg
      361 tcaccttgcg attaagtacc aagggtatga gaattattga caagttaggc ataaaggcag
      421 ttctggataa aatcagagct aaaggcgaga agatataaca aggggataag ccatggcagc
      481 tgttacaatt aaagtgaaaa tggaatccac agcaggaact ggatactata aaaccactac
      541 taaaaatcca cgtaaccatc ctgaaaagat ggaattaatg atgtatgacc ctaaagtacg
      601 aaagcacgta ctgttcaaag agaaaaaagt aaaataaatt gataggcccc tgtctttggg
      661 gcttacctcc tctcattatc cactttaaaa accaatttgt tatatttcaa aacaagccta
      721 tcattttatt aaaaatatag gtaattccat tacttttaaa ttcaaattaa cgcccgtaag
      781 cactggataa tcctcgtgga tcgtttaaac tgatagttaa agacagtgaa aaaaaaaaga
      841 ggatttgaat gaaaccgaca ttgcagattg gtattggtca acatctcacg ttaaccccac
      901 aactgcaaca agcaataaga ctgctgcaat tatccacctt tgatttacaa caagaaattc
      961 aactcgttgt tgaatcaaat cctttgcttg aagccactcc tgttgaagaa aaagaagacg
     1021 tccaaataaa taccaatcag aaccaagatg aatattcaga ttttcaatgg tcacagctgt
     1081 ataataactt aaacaagcgc aataattttg aagaaaacga ttacaatttc gataatttac
     1141 actgcactac caccaattta caagatcatc ttaactggca acttgacctg acgcctatga
     1201 gcgatattga ccgagtcatt gctacaacaa ttattgatgc gataaacgat gacggctttt
     1261 taaccatacc cgttgctgaa attcacgcca gcttaaatag cgatgatttt cctctggata
     1321 tagaagaaat agaagcagtg aggcacaggc ttcaattatt tgaccccgta ggatgcgcat
     1381 ctgttaattt agcagaaaca ctcgttttgc aattggaaca acttccactc gaacccaagc
     1441 ttttggctac tgccaagaaa gtcattcttg aagacattga gttgctaggg caacataact
     1501 acagacaact catgaaaaat cat
//
LOCUS       DASLOE010000302         1511 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.304, whole genome shotgun sequence.
ACCESSION   DASLOE010000302 DASLOE010000000
VERSION     DASLOE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.304"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            64..1308
                     /locus_tag="U6309_002973"
     CDS             64..1308
                     /locus_tag="U6309_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEN5506375.1"
                     /translation="MSDLKNLILNSSILLDTSSTVQNDLNPEQLINVHEDSDGDSDLL
                     TPESFILLMAEILSTNNADTICEYEHQNSADNKVLQNLQFKEVEEDEQIEGLNEQKKS
                     LDNPLWHDVDLPVINEISGNQCEGQFESTLEKNVALTWINSENFQPPIVKPENQTDET
                     YKIWDSEITSIESGKLKLDFPKADYDGNNINPYQKEAINQPGNDSSKNHQLIKGNDSF
                     PILEDIKTNAEPGTKAIKSEVEATKVGTEIPGSSFQMNQKAGSDIQPPVVSNSHIPKN
                     FSIPIHVNHSQWSEQLSEHIVWLGHQDVKSALIKIHPEELGPLEINVKVVKDNASVNI
                     STHSVYVKEIVDQAIPRLREMMAQQGINLSEVHIGTDTSSRQGSQKNHHAEIDLIQSA
                     EDKNKITSLTRHLPKGLVDYFA"
BASE COUNT          554 a          256 c          270 g          431 t
ORIGIN      
        1 ttcagtaagc ttgcagatgg tatgagtttt gctatatgtt aatcataaca cagggagtgt
       61 gatatgtctg atctgaaaaa ccttatactc aattcatcca tcttattgga tacctcttca
      121 acagttcaaa atgatctaaa ccctgagcaa ttgattaatg ttcatgaaga ttctgatggt
      181 gacagtgatt tattaactcc tgaatctttt atactgctaa tggccgaaat attatcaaca
      241 aataatgcag atacaatatg cgaatatgag catcaaaatt cagcagataa taaagttttg
      301 caaaatttgc aatttaaaga ggtggaagag gacgaacaaa ttgaaggact caatgagcaa
      361 aaaaaatcct tagataatcc attgtggcat gatgttgatt tacctgttat taatgaaata
      421 tcaggtaatc aatgtgaagg gcagtttgaa tcaacactag aaaaaaatgt ggctttgaca
      481 tggattaatt ctgaaaattt ccaaccacca atagttaaac ctgaaaatca gacagatgag
      541 acttacaaaa tttgggactc ggaaataact tctattgaat ctggcaaact gaaactggat
      601 tttccaaaag cagattacga tgggaataac attaaccctt atcaaaaaga ggcaatcaat
      661 caaccgggga atgattcatc gaaaaatcat caattaatta aagggaatga ctctttcccg
      721 atattagaag acattaaaac gaatgctgag ccaggaacta aagcaataaa atctgaagta
      781 gaggcaacta aagtgggcac tgaaatacct ggtagttcct ttcaaatgaa ccaaaaagcc
      841 ggttcagata ttcaaccccc agttgtttcc aactctcaca tacctaaaaa tttttcaatt
      901 ccaattcatg tcaatcattc acaatggtca gagcaattat ctgaacatat tgtttggctt
      961 ggtcatcaag atgtaaaaag tgccttaatt aaaattcatc ctgaagaatt ggggcctttg
     1021 gaaattaatg taaaagttgt aaaggataat gcctctgtta acatatctac tcacagtgtt
     1081 tatgtaaaag aaattgttga tcaagcgatt cccagattaa gagagatgat ggctcagcaa
     1141 ggtataaact tgtctgaggt gcatattgga accgatacaa gttcccgcca aggttcgcag
     1201 aaaaaccatc atgctgaaat agatcttatc caatcagcag aagataaaaa taaaatcact
     1261 tcattaacca ggcatctacc caaaggactg gtcgactact ttgcttagtt taacaaatca
     1321 attgttggta aggagtttac aacagtgtcc cataattatt ttcgtacctg tctgacgaca
     1381 aaggacaata agtttttttg gcaaaaacct ttggcatcag aacggtttga caagctgatt
     1441 gctgatagaa aatttcaaaa atcaatagct cacagcagga aaaatcaatt aatgtatagt
     1501 tttatagagt c
//
LOCUS       DASLOE010000303         1414 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.305, whole genome shotgun sequence.
ACCESSION   DASLOE010000303 DASLOE010000000
VERSION     DASLOE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.305"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..281)
                     /locus_tag="U6309_002974"
     CDS             complement(<1..281)
                     /locus_tag="U6309_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN5506376.1"
                     /translation="MIQLEESKMIHILYKQGYSKKAIARKLGMSINTVRKYLKSGSEP
                     YYKRRDKKPLKLDPYKAYIQKRLKDATPHWVPATVIYREIKLFGYDGCSS"
     gene            260..943
                     /locus_tag="U6309_002975"
     CDS             260..943
                     /locus_tag="U6309_002975"
                     /inference="COORDINATES: protein motif:HMM:NF033520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN5506377.1"
                     /translation="MTPLIESFPPPEFILLKKTIVGGSIFSRQRGSVYIQQRQGLYYV
                     DSTTLKVCHNKRIHRHKTFAGIAERGKSSMGWFFGFKLHLVINHLGELIAFCITKGNV
                     DDRQPLEQLFKNLHGLAAADKGYLSKKKEESLFQKGVKLITKVRRNMKAKILSTFEKY
                     FLGQRSIVETVIEQLKSICQIEHTRHRKPDNFVINLLSGLAAYMLKPRKPSLKIHSLN
                     KNLGLLMSS"
BASE COUNT          448 a          267 c          239 g          460 t
ORIGIN      
        1 gagctgcatc catcataacc aaacaattta atctctcgat aaatcacagt agctggtacc
       61 caatgaggtg ttgcatcttt taatcttttt tgaatatatg ctttataggg gtcaagcttt
      121 aatggctttt tatcacgtct tttgtaataa ggctctgaac cacttttaag atattttcga
      181 acagtattta tcgacatacc caattttcta gcaatcgctt ttttactgta tccctgctta
      241 tacaaaatat gaatcatttt tgactcctct aattgaatca ttccctcctc cagaatttat
      301 tctcctgaag aaaactatag tagggggatc aattttcagt cggcaaaggg gatcagttta
      361 tattcagcaa cgacaagggc tgtattatgt tgattcaacc accctgaaag tatgtcataa
      421 caagcgtatt catcgccata aaacatttgc tggcatagcg gaacgtggca aatcctcaat
      481 gggctggttt ttcggtttta agctccatct tgtgattaat catcttggtg aattaattgc
      541 tttttgcata acaaaaggca atgtggatga ccgtcaacca ctagagcagc ttttcaaaaa
      601 cctccatggt cttgccgcag ctgataaggg gtatctttca aagaaaaaag aggaaagcct
      661 tttccagaaa ggagttaagc tcatcacaaa ggtacgcaga aacatgaagg ctaaaatact
      721 ctcaactttt gaaaaatatt tcctcggaca acgcagtatc gttgaaacag taattgagca
      781 attaaaatca atttgccaaa ttgaacatac cagacatcgt aaaccagata attttgtcat
      841 taacctttta tccggcttag ctgcttatat gctcaaacca agaaaacctt ccctcaaaat
      901 ccattccctt aataaaaatt taggcttact tatgtcgagc tgaggttaat ttatatattt
      961 tgattatctt tttagcagta tttaagcctt ttggaagaga cgtaataatt atccatggct
     1021 ccttagctcc tttagcatat ttcttactat tgcctgattg acactttact ccttgaagcg
     1081 tcttttttat acgaccttta ctacgttcct tatagatata aaggtttgca ttaagtggat
     1141 ttgctctggc aacggtcgcc cgatttaggt atttaacgtg aattcgatat aactgtctaa
     1201 aaaacaaact taagaatggt aacatattga aaaaccagac catttgtgat cttattcgtt
     1261 ttttcgaaga acaaaaataa gaatcaagag gtctggagtg gataaattaa tagaattatt
     1321 ctgtgttgtc gatgattttt gccaaaaata tatgccacga tttgagcaac atctgataaa
     1381 tatttcaggt aagaccagaa gaaaaccatg cagc
//
LOCUS       DASLOE010000304         1403 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.306, whole genome shotgun sequence.
ACCESSION   DASLOE010000304 DASLOE010000000
VERSION     DASLOE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.306"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..223
                     /locus_tag="U6309_002976"
     CDS             <1..223
                     /locus_tag="U6309_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rab1 guanine nucleotide exchange factor DrrA"
                     /protein_id="HEN5506378.1"
                     /translation="FKDKLAEATDEQSLKQIVAELKGKDEYKILAKGQGLTTQLLGLK
                     TSSVSAFEKMVEETRESIKSQERQTIKIK"
     gene            complement(390..1109)
                     /locus_tag="U6309_002977"
     CDS             complement(390..1109)
                     /locus_tag="U6309_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEN5506379.1"
                     /translation="MVDYHYFKSKLNHFYGRYVGYNQRPVFFDIDQTFPELNLITHHF
                     PDIKREFENVQANVKEFPRYHDIDPGETAISNSTEKNWNVFMLYLLGYQLKEARLLCP
                     TLCSLLEGIPHLIQAFFSILEPGKSIPLHEGPYIGYLRYHLGIHVPKNNPPQIFVNNQ
                     PYTWKEGEAVLFDDSWPHEVRNTSDDYRAVLIIDVLRPMPFLPQLVNQFVTHVIARYF
                     YGRKVMKRAMQYGGEVKPLAL"
BASE COUNT          453 a          223 c          274 g          453 t
ORIGIN      
        1 tttcaaggat aaactggctg aagctaccga tgaacagagc cttaagcaaa ttgttgctga
       61 attgaaaggt aaagatgaat ataaaatttt ggctaagggg caagggttaa caacccaact
      121 tttagggcta aaaacaagtt ccgtgtccgc atttgagaaa atggttgagg aaacaagaga
      181 aagtattaag tctcaagaaa gacaaaccat taagataaaa taactagcgc atttcaatta
      241 tgaagatatg cttatatcgc tctttttagt atagagaaag actgataaga gctaattcaa
      301 taagggttga acctaaagtt caacttttat ttttttattg attgataaat attttaaacg
      361 attttcgaaa gataataatc gttgtttact taaagtgcca aaggctttac ttctccacca
      421 tactgcatcg cgcgtttcat tactttgcga ccataaaaat atcgagcaat gacgtgggtt
      481 acaaattgat tgaccagttg cggcaagaaa ggcatagggc gcaagacatc aatgattaat
      541 acggcacgat aatcatcact tgtatttctt acttcatgtg gccaggagtc atcaaaaagt
      601 actgcttctc cctctttcca agtgtaaggc tggttattaa caaagatttg tggtggattg
      661 tttttaggga catgaattcc taaatgatat cttagatagc caatataggg gccctcgtgt
      721 agaggaattg attttccagg ttctaaaatc gagaaaaaag cttgtataag atgtggtatt
      781 ccttccaata aactacaaag tgtcgggcat aataaacggg cttccttgag ttgataacca
      841 agaagataca gcatgaatac attccagttt ttttctgtgg agtttgatat ggcggtttct
      901 ccaggatcta tgtcgtggta acgtggaaat tctttcacat tcgcttgtac attttcgaat
      961 tcccttttga tatcaggaaa atgatgtgtt atgagattta attctgggaa agtttgatca
     1021 atatcaaaaa aaaccgggcg ttgattatat cctacatagc gcccataaaa gtgattcaat
     1081 ttactcttga aataatgata gtctaccatt gcttattcct ttattttatt tgtgataata
     1141 atcaggtgcc gaatgttcga ctatccttgg gaaaatcagc tattttaaaa tgaagtttga
     1201 agaaatggaa gtttaaaaaa tgctcgtttt tgtgccgagg catcctttca ggttttggaa
     1261 atgatttttg gcatccagct tattccaagt aagcatattc tcaatggcaa tcatcatatg
     1321 acatttatcg ctatggctta aaaaaaatac ggataacaat ttcctttttc ttgctatttt
     1381 tcagctatta cggttgggaa gag
//
LOCUS       DASLOE010000305         1393 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.307, whole genome shotgun sequence.
ACCESSION   DASLOE010000305 DASLOE010000000
VERSION     DASLOE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.307"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..933
                     /locus_tag="U6309_002978"
     CDS             <1..933
                     /locus_tag="U6309_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5506380.1"
                     /translation="FFPIKNRDVLLKSPSFNHVGSEFQPMVSRRDVNSISITLPIKHN
                     IHRNHNSNLSVDSTSAVDSSNQQDHISYLHICFTHEEGKWYLFLKCFTTLDVFNYDFA
                     PLLKILHKPDIRTYPMSLGCPGVIDRTELVNMDRTIYVRQFIIHGPDDEAKILQDIQD
                     FNMNCLRRFEELTYGRDNSAYRNSHALGRILHKIITAYQEVTVNEYDIALNRLKIVFQ
                     DQVFQNDPQICLSLTHIVNALIVLSQKKELNNKGVEIDEHQLHLILKELAKHEGKKFK
                     NNLWFPIGTVDAIRQELEELFISKQTISAASIMS"
     gene            980..>1393
                     /locus_tag="U6309_002979"
     CDS             980..>1393
                     /locus_tag="U6309_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain protein"
                     /protein_id="HEN5506381.1"
                     /translation="MKQIKGMLLSIISLSFLSFLVFASDASISLVGVQGYDLVSYHQK
                     NGPVRGSGNHTAYHNGVAYLFATDENKKVFQANPEKYLPAYGGYCAFAVSVGRKVVSD
                     PLAWKIVDGILYLNLNKQVQEFWSKDIPGNIKKGNI"
BASE COUNT          475 a          209 c          256 g          453 t
ORIGIN      
        1 ttttttccaa taaaaaatag agatgtttta ttaaaaagcc cctctttcaa tcatgtcggt
       61 tcagaatttc aacccatggt atccaggagg gatgtaaact caatctcgat tacattacca
      121 attaaacaca atattcatcg aaatcataat agtaatttgt cagttgactc aacatcagcc
      181 gtggactcat ctaatcaaca ggatcatatt tcctatttac atatatgctt tactcatgag
      241 gaaggaaaat ggtatttatt tttaaagtgt ttcacaacgc tggatgtttt taattatgat
      301 tttgcaccat tattgaaaat attacataaa ccagacataa gaacttatcc catgagtttg
      361 ggttgccctg gggttattga taggacagaa ctcgttaaca tggatagaac aatttatgtc
      421 aggcagttta ttatacatgg gcctgatgat gaggctaaaa tcttgcaaga tattcaggat
      481 ttcaatatga actgcttgag gcggtttgaa gagttaacct acggtagaga taatagtgct
      541 tataggaact ctcacgctct ggggagaatt ttgcataaaa ttataactgc ttatcaagaa
      601 gtaactgtaa atgaatatga tatagcattg aaccggctta agattgtgtt tcaagatcaa
      661 gtatttcaaa atgatccaca aatttgtttg tctttgaccc acatagtcaa tgcattgatt
      721 gttttaagtc aaaaaaagga acttaataac aaaggagtgg aaattgatga acaccaatta
      781 catctcattt taaaagaact tgcaaaacat gaaggtaaaa aatttaaaaa taatctatgg
      841 tttcctatag ggactgttga tgcaattaga caagagttag aagaattgtt tatttcaaag
      901 caaactatat ccgcagcatc tataatgagt taatagtgat taaatactcc cagttacaat
      961 ttaattaagg acgataaaaa tgaaacaaat taaaggaatg ctgttatcaa taatatcttt
     1021 atcattcttg tcgtttctgg tgtttgcttc tgatgcttcg atcagtctgg ttggcgttca
     1081 aggttatgat ttggtttctt atcatcagaa aaatggccct gtccgtggta gtggtaacca
     1141 cactgcctat cataatggag tagcttacct atttgctact gatgaaaaca agaaagtgtt
     1201 tcaggctaat cctgaaaaat atttgccagc atatggtggt tattgtgctt ttgcagtttc
     1261 agtagggcgg aaagtagtga gtgatccatt agcatggaaa attgttgatg gcattttata
     1321 tctcaatctt aataaacagg tacaagaatt ttggtcaaaa gatattcctg gaaacataaa
     1381 aaaaggtaat att
//
LOCUS       DASLOE010000306         1380 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.308, whole genome shotgun sequence.
ACCESSION   DASLOE010000306 DASLOE010000000
VERSION     DASLOE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.308"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            54..>1380
                     /gene="ceg32"
                     /locus_tag="U6309_002980"
     CDS             54..>1380
                     /gene="ceg32"
                     /locus_tag="U6309_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Ceg32/SidI"
                     /protein_id="HEN5506382.1"
                     /translation="MTKIYLLTAPFDEEAKTGDGQYAASLELGFAENYEHIPCKWLKK
                     DNGDYSIPFPTGTTSIPEETIPSAIVLQPVANENTIISGYKLKDAVSSPEKAQEVNNK
                     TASPRTPKIIVKHDNSLQSLTIMDIYSQKPIQFDESKVDEIINSLETKKVNLEKAIED
                     NNAELDKIKKQKSKLAYLTRLYKENKENIQDYCTLNEYIEAHLFNPKFLSRHEKALNN
                     FKALKSQFTGPVNLKELEKLTDKLTGIKEYSYDFHSNSLPYDLEHDKSFRNFYDFDGL
                     KESIESIIKELEVLNSIRQAVSDKYPNSFKALNETEEHDDKLKFINIIFNDGFSTTYD
                     QQTFIKALSALDIEKAIDAYTNVKNKLENTQDIIANKEGCRNKLISELQTLIANKQEP
                     YLSANEKLGGFYSKRKLSASEGFHLAYQANRRDPIKPEVIENIITKMKPI"
BASE COUNT          563 a          232 c          218 g          367 t
ORIGIN      
        1 aataatctta atgtaagata ttcaccatga caaaaatttt actaaagtaa tttatgacta
       61 aaatatattt attaacagcg ccttttgatg aagaggcaaa aactggtgat ggacaatacg
      121 cggcctcttt agaattgggt tttgctgaaa attacgaaca tataccctgt aaatggttaa
      181 aaaaagacaa tggtgattac agtatcccat tcccaacagg aactacttcc ataccggaag
      241 aaacgatacc aagcgcgata gtattacagc cagtcgctaa cgaaaataca atcataagcg
      301 gttataaatt aaaagatgct gtttcatctc cagaaaaagc acaggaagta aataacaaaa
      361 cagccagccc cagaacacca aaaattattg taaaacatga taacagcctg caatcactta
      421 caataatgga tatttactct caaaaaccta tccaatttga tgaatcaaaa gttgatgaaa
      481 taattaactc tttagaaacc aaaaaagtaa acctggaaaa agctatagaa gataataacg
      541 ctgagctgga taaaattaag aaacaaaaat ccaaattagc gtatttgact agattatata
      601 aggaaaataa agagaatatt caagattatt gcaccttaaa tgaatatatt gaggctcatt
      661 tatttaatcc taaattttta agcagacatg agaaagcact caataatttt aaggcactta
      721 aaagccaatt tactggccct gtcaatttaa aagaactgga aaaattaaca gataaactga
      781 ctggaattaa agagtacagc tacgatttcc atagtaacag tttaccctat gatttagagc
      841 acgataaaag tttcagaaat ttttatgatt ttgatggatt aaaagaatct attgaaagca
      901 ttatcaagga attagaggtg cttaacagca ttcgtcaggc tgtatcagac aagtacccca
      961 atagctttaa agcgctaaat gaaacagaag agcacgacga taaattaaaa tttatcaata
     1021 ttattttcaa cgatggattt agcaccactt atgaccaaca aacctttatc aaggccttat
     1081 cagctcttga tattgaaaag gcaattgacg cttatacaaa tgttaaaaac aaactggaaa
     1141 acactcagga tattatcgcc aataaagagg ggtgtagaaa taagttaatt tcagaattac
     1201 aaactttaat tgcaaataaa caagaacctt acctctctgc aaatgaaaag cttggcggtt
     1261 tttacagtaa aagaaaactc tccgcaagtg aaggatttca tttagcctat caggcaaacc
     1321 gacgtgaccc gataaaacct gaagtgattg aaaacattat taccaaaatg aaacccattg
//
LOCUS       DASLOE010000307         1376 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.309, whole genome shotgun sequence.
ACCESSION   DASLOE010000307 DASLOE010000000
VERSION     DASLOE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.309"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..283
                     /locus_tag="U6309_002981"
     CDS             <1..283
                     /locus_tag="U6309_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lpg2461 family Dot/Icm T4SS effector"
                     /protein_id="HEN5506383.1"
                     /translation="VEEIAKTLIHEVCHFLNSDLYDKEMETEESKLVGYKDEVRAFTA
                     EKMFEKNKGYLLRSDINQIHETVTELYPEFTDPENPISGYIYSTFDSPS"
     gene            490..>1376
                     /locus_tag="U6309_002982"
     CDS             490..>1376
                     /locus_tag="U6309_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN5506384.1"
                     /translation="MSKQEHIISHNTDSSHLNTVMQQMKERIINEGDKPHVTVEAQLK
                     ILQELSQFRFGQFLIKNQGINGYWTHYMLTHPWFGRKTGLNNSREPLSNMECFLLDKA
                     PTMLATQQRFELFLQENQKALVNGATLASIPCGMLGELLYLDFNGINNIRLIGIDYDQ
                     AALEDANALAKERNLDRFTTLVCRNAWELNIQEEFDLISSNGLNIYEPDDNKVLDLYQ
                     QFHTALKKGGKLVTSFLTPPPHLSSQCEWIFNKINPEDLLLQRIIFSDILNAKFQCYR
                     SSQQTQKQLALAGFNNIHFL"
BASE COUNT          483 a          252 c          245 g          396 t
ORIGIN      
        1 cgttgaagaa attgccaaaa cattaatcca tgaagtctgt cattttttaa attcagatct
       61 ttacgataaa gaaatggaaa cagaagagtc aaaactagtc ggctataaag atgaagtaag
      121 ggcatttaca gcagaaaaaa tgtttgaaaa aaataaaggc tacctgctca gaagcgatat
      181 aaaccaaata cacgaaacgg ttaccgaact atatcctgaa ttcaccgatc cggaaaaccc
      241 aatatcaggg tatatttaca gtacttttga tagcccttct taagggctgt ttacattttg
      301 ccagcttgag tcgaaaagcc ttgattttcg atcgccgtag tggaacgtac aatgaaatga
      361 gttaaaaaat ctacgtatct tcaagaatat taagtataat tgctctgagt ttgaattata
      421 aaataaatcc attttcttat cttaaactca cagcagttaa taattataaa gattatccag
      481 agagcctgta tgtcaaaaca agaacatatc atttctcata acaccgattc atctcatctg
      541 aatactgtaa tgcagcaaat gaaggaaaga ataataaacg agggtgataa gccacatgtc
      601 acggtagaag ctcaacttaa gattcttcaa gaattaagcc aatttaggtt tggtcaattt
      661 ctaataaaaa atcagggtat caatggttat tggactcatt atatgttgac tcatccttgg
      721 tttggaagaa agacggggtt aaacaatagt cgcgagccat tatcaaacat ggaatgtttc
      781 ttgttggaca aagcaccaac aatgttagca acccaacaac gtttcgaatt atttttgcag
      841 gaaaatcaaa aagctctggt taatggcgca acccttgctt ctatcccctg tgggatgtta
      901 ggggaattac tttatcttga tttcaatggc atcaacaaca tccgactgat aggtatagat
      961 tatgaccagg cagcattaga ggatgccaat gccttggcaa aagaacgaaa cctggacaga
     1021 ttcacaacgc ttgtttgtcg taatgcctgg gagcttaata ttcaagagga atttgatctc
     1081 atttccagca atgggttaaa tatttatgaa ccagacgata acaaagttct ggatttatac
     1141 caacagtttc atacggcctt aaaaaaaggc gggaaattag taaccagttt tttaactcct
     1201 ccccctcacc tgagttcaca atgcgaatgg atattcaata aaattaaccc agaagattta
     1261 ttactgcaga gaattatttt ttccgatatt ttaaatgcaa aattccagtg ttatcgctca
     1321 tcacaacaaa cccagaagca actcgcattg gcaggcttca ataatattca ttttct
//
LOCUS       DASLOE010000308         1341 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.310, whole genome shotgun sequence.
ACCESSION   DASLOE010000308 DASLOE010000000
VERSION     DASLOE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.310"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..218
                     /locus_tag="U6309_002983"
     CDS             <1..218
                     /locus_tag="U6309_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HEN5506385.1"
                     /translation="ILFVDDAKRNIVSMDKAFANRDEFLVLNVFYTKENAKELEVQTN
                     PHLQAQVFEQWNFIKENVNNVIIQSNF"
     gene            complement(402..758)
                     /locus_tag="U6309_002984"
     CDS             complement(402..758)
                     /locus_tag="U6309_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506386.1"
                     /translation="MFSGNPQKGYLLMSDSAGKLKTPKFKKLRGKLWEYLKELSALPI
                     KHGLELAIKQIKRSDIVVSRNKGQNYGKLFDDAKRRKNYQHTSDLMLFRGIFIMKQLK
                     VCLIDLNYHQKQLCYF"
     gene            complement(769..1080)
                     /locus_tag="U6309_002985"
     CDS             complement(769..1080)
                     /locus_tag="U6309_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEN5506387.1"
                     /translation="MQIIKSSLFTGDFAWQALDIAQISDASIRLHWADQPYIWHKNDG
                     DEVFVVLSGKVIMHYREGGVENQKELNSGDICYLKEGCEHVAYPKGAARILVIEKAGS
                     I"
     gene            complement(1096..>1341)
                     /locus_tag="U6309_002986"
     CDS             complement(1096..>1341)
                     /locus_tag="U6309_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506388.1"
                     /translation="LDEHQNLEYIKKLISFVTLKLNVRPGKYLKKTVDLFSMPGLLIL
                     SHESKEQVESDYSLVRKLEQKGLFVLAASQESKTIKI"
BASE COUNT          426 a          268 c          193 g          454 t
ORIGIN      
        1 ctatcctttt cgtcgatgat gcaaaaagga atatagtatc tatggataaa gcctttgcaa
       61 atcgtgatga gttcctggtt ttgaatgtat tctacaccaa agaaaatgcc aaggagctag
      121 aggttcaaac aaatccgcat ctgcaagcac aagtatttga gcaatggaat tttatcaaag
      181 agaacgtaaa taatgtcatt attcagtcaa atttctaaaa aaactaatat ttgtagtgaa
      241 tttctgcgat aaaaattaca atataatcaa ttgcttattt tggcattgag gggctcataa
      301 tccgttgttc ctgggctcaa gccctggtgg gcccaccaat ttaattttaa aatcaataag
      361 ttaaacctgc ccttcccaac ccgttgcata gagatcaaaa ctcaaaagta gcatagttgc
      421 ttttgatgat aattcaaatc tatcaagcaa accttgagtt gcttcataat aaatattccc
      481 ctaaacaaca tgaggtcaga agtatgttga taattttttc ttcgcttcgc atcatcaaac
      541 aattttccat agttctgccc tttatttctg gatacaacaa tatcagatct tttaatttgc
      601 ttgatagcta attctaaacc atgcttaatc ggcagtgcag aaagctcttt gagatattcc
      661 catagcttgc ctcttaattt tttaaattta ggagttttta acttgcctgc cgagtcactc
      721 atcaataaat atcctttttg aggattacct gaaaacataa atcaattttt aaatactacc
      781 agctttctct atgacaagta ttcttgcagc cccttttgga taagctacat gctcacatcc
      841 ttctttcaaa taacatatgt ccccactatt taattctttt tgattttcta caccaccctc
      901 acggtagtgc ataatcactt ttccagacag cacaacaaaa acctcatccc catcattctt
      961 atgccaaata tatggttgat ccgcccaatg aagtctaatg ctcgcatcag atatttgagc
     1021 aatatctaat gcttgccatg caaaatcgcc ggtaaataat gatgatttaa ttatttgcat
     1081 attaccttac gcttattaaa ttttaatggt tttagactcc tggctagcag ccagaacgaa
     1141 taatcctttt tgctccaatt tcctgactaa actataatca ctttcaactt gttctttcga
     1201 ttcatggctt agaattaata gaccaggcat ggaaaaaaga tcgactgttt tcttaagata
     1261 cttacctggt cgaacgttaa gtttaagagt tacaaaacta attaattttt taatatactc
     1321 tagattttga tgttcatcta a
//
LOCUS       DASLOE010000309         1315 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.311, whole genome shotgun sequence.
ACCESSION   DASLOE010000309 DASLOE010000000
VERSION     DASLOE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.311"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(7..1203)
                     /locus_tag="U6309_002987"
     CDS             complement(7..1203)
                     /locus_tag="U6309_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISLpn1 family transposase"
                     /protein_id="HEN5506389.1"
                     /translation="MHKKIFCQNLLDSALKNSIHAKRKQCLVRFLSDLMDYDTTLSVT
                     EIGKKLTSKTTVKSKIYAAQTFVNNFKLERDIVCIYKSLAHFFWSHAKEIVVLIDWTG
                     GCSEGYHVLEASIAAHGRSIPIYHEVHSESEQENAEIHRQFLLRLKEVIPSSLSVTII
                     TDAGFHREWFQQVLELGWDVIGRIYSLYCYQIEGETNWHKVKDILFEGIGKASALGKV
                     KLGKTKKAVEGYLYTYKEKLSGKVRKKKNKYPSHDKAHSNYYKNGWVLFSSLNKHARF
                     LVSYYKKRMQIEQNFKDIKNEQLGMGLRRNQSSGKTRVNMLFFLAVLLIMIAWWFGLM
                     IESLNKHRSYQANTIKNKRVRSFIHLARMAYRHEPELLNWDLFQNIMSDLKQQYHSFI
                     ECGALS"
BASE COUNT          409 a          258 c          196 g          452 t
ORIGIN      
        1 ttttactcag ctcaaagcgc cacattcaat aaatgagtga tattgctgtt tcaaatcact
       61 cattatgttt tgaaataaat cccaattcag taattccggc tcatgtcgat aagccattct
      121 agcaagatga ataaaagagc gaactcgttt gttttttata gtatttgctt gataagagcg
      181 atgtttatta agggattcaa tcattaaacc gaaccaccag gcaatcataa ttaataaaac
      241 agcaagaaaa aaaagcatat taactctggt ttttcctgaa gattgatttc gtcgtaatcc
      301 cattcccagt tgttcatttt ttatgtcttt gaaattttgt tcgatttgca ttcttttttt
      361 gtaataagac acgagaaatc gagcatgctt atttaatgag ctaaagagaa cccacccatt
      421 tttataataa ttggaatgag ctttatcatg agaaggatat ttatttttct tttttcgtac
      481 cttgcctgag agtttttctt tataggtata aagataacct tccaccgcct tttttgtttt
      541 tccaagcttg accttaccta atgcagaagc ttttccaata ccttcaaaaa gaatatcttt
      601 cactttgtgc caatttgttt ctccttcaat ctgatagcaa tacaaagaat agatgcggcc
      661 aataacatcc cagcctagct caagcacctg ttgaaaccac tcacgatgaa aacccgcgtc
      721 tgtaataatt gtcacggaca aactcgaagg aataacctct ttcaagcgta ataaaaattg
      781 acgatgaatc tctgcatttt cttgctctga ctcagaatga acttcgtgat agattggtat
      841 tgagcgacca tgagctgcaa tactagcttc cagaacatga tagccctcgc tacatcctcc
      901 agtccaatca attaaaacaa caatctcttt tgcatggctc caaaaaaaat gggccagact
      961 cttataaatg caaacaatat ccctttccag tttgaaatta ttcacgaatg tttgagccgc
     1021 atatatttta cttttaactg tcgttttgga tgttaatttt ttaccaatct ctgtaacgga
     1081 taatgtggta tcgtagtcca ttaagtcact taaaaagcga accaaacatt gttttctttt
     1141 agcatgaatt gaattcttta atgcgctatc caataaattc tggcaaaata ttttcttgtg
     1201 catacaactt ccgattagtt ttgtcttggt aaacaaattt gatcatgaat ctgtatgcac
     1261 ttcaatttct tttcccatct ttgttaatgt acactttaaa aaagtaactg ctcaa
//
LOCUS       DASLOE010000310         1309 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.312, whole genome shotgun sequence.
ACCESSION   DASLOE010000310 DASLOE010000000
VERSION     DASLOE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.312"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>1309
                     /locus_tag="U6309_002988"
     rRNA            <1..>1309
                     /locus_tag="U6309_002988"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          361 a          257 c          418 g          273 t
ORIGIN      
        1 aatcctggtt gaagatgggt ggaccatcat ccaaggctaa atactactta ctgaccgata
       61 gtgaaccagt accgtgaggg aaaggtgaaa agaaccccgg agaggggagt gaaatagaat
      121 ctgaaaccgt ttgcgtacaa gcagtgggag catggtttag gctgtgtgac tgcgtacctt
      181 ttgtataatg ggtcagcgag ttactttcag tggcgaggtt aactgaataa gggagccgta
      241 gagaaatcga gtctgaatag ggcgatagtc gctgggagta gacccgaaac cgggcgatct
      301 agccatgtgc aggatgaagg ttgggtaaca ccaactggag gtccgaaccg ggtaatgttg
      361 aaaaattatc ggatgacgtg tggctaggag tgaaaggcta atcaagcccg gagatagctg
      421 gttctccccg aaagctattt aggtagcgcc tcgtgaatga ttactggggg tagagcactg
      481 tttcggctag ggggctgtca tggcttacca aaccgatgca aactccgaat accggctaat
      541 tgaatcacgg gagacacacg gcgggtgcta acgtccgtcg tggagaggga aacaacccag
      601 accgccagct aaggtcccca agtactagtt aagtgggaaa cgatgtggga aggcatagac
      661 agccaggagg ttggcttaga agcagccacc ctttaaagaa agcgtaatag ctcactggtc
      721 gagtcggcct gcgcggaaga tgtaacgggg ctaaaactag tcaccgaagc tgcggatgtg
      781 cgcgaaggcg cacgtggtag gggagcgttc tgtaggctga tgaaggtgca ttgagaagtg
      841 tgctggaggt atcagaagtg cgaatgctga catgagtaac gataatgtgg gtgaaaagcc
      901 cacacgccgg aagtcccagg tttcctgcac gacgttaatc ggagcagggt gagtcggccc
      961 ctaaggcgag gctgaagagc gtagtcgatg ggaaccaggt taatattcct ggacttttta
     1021 taagtggtga agtggggacg aagaaggcta ggtgagccag gcgttggttg tcctggtact
     1081 tgcatgtagg ggggaagact tggcaaatcc ggttttccat aactctgagg tgcgaagtgg
     1141 ttctgacctt ttggtacaga gaagtcattg atgcccggct tccaggaaaa gctgctagcc
     1201 ataacttata gagaaccgta ccgcaaaccg acacaggtgg acaggtagag aatactaagg
     1261 cgcttgagag aactcgggtg aaggaactag gcaaaatggt accgtaact
//
LOCUS       DASLOE010000311         1300 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.313, whole genome shotgun sequence.
ACCESSION   DASLOE010000311 DASLOE010000000
VERSION     DASLOE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.313"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>1300
                     /gene="vpdA"
                     /locus_tag="U6309_002989"
     CDS             <1..>1300
                     /gene="vpdA"
                     /locus_tag="U6309_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm type IV secretion system effector VdpA"
                     /protein_id="HEN5506390.1"
                     /translation="KTKQEVSQQDKLKDSKSSTPLQTKETWFISDTLNIIFDSYDFSI
                     SVIEQAPMPYRIVFSGGGSRILAHIGAMDELTRHGLQFTEFSGSSAGAMVAAFAYLGY
                     NCSEIKQIISWFNEDKLLDSPLIFNFNNIKQIFNKGGLSSAKLMRQAANYVILKKVMD
                     IISDEKFKTRFAQFQNFLEENIYSCPENITFQTLARIKEICPECELGEKLFITGTNLS
                     TQKHEVFSIDTTPSMALADAIIISANLPIAFERICYQGNVYSDGGISNNLPAHCFSEK
                     GHKTTFLKHKDDVDFSVLALQFDNGLEENALYSQNPIPKWSWLSNTFYSLITGHPNVT
                     ENWHEDLQILRRHAHQSILIKTPTIALTNLTISQDTKKALVESGRTAAKNYLDLHEFC
                     MDDYGNIRHNECLHEKFQKPEELLDYCVLHSHFELLKKIKQ"
BASE COUNT          459 a          245 c          223 g          373 t
ORIGIN      
        1 aaaacaaagc aagaagtttc tcagcaggac aaattaaaag attccaaatc gtctacacca
       61 ctacagacaa aagaaacatg gttcatctct gataccttaa atattatttt tgactcctat
      121 gattttagta tctctgtcat tgaacaagct cccatgccat atcgtattgt tttcagtggc
      181 ggaggctcaa gaatattggc acatataggt gccatggatg agttaaccag gcatggatta
      241 cagttcactg aattttccgg aagctcagcc ggtgcaatgg tcgctgcctt tgcttatctt
      301 ggttataatt gttccgaaat aaaacaaatt atttcctggt ttaatgaaga taaactcctt
      361 gatagcccac tcatttttaa tttcaataac ataaaacaaa tatttaacaa gggggggctt
      421 agctcggcaa aattaatgcg tcaagcagca aattacgtta ttttaaaaaa agtaatggat
      481 attatatccg atgaaaaatt caaaacacga tttgcacaat ttcaaaattt tttagaagaa
      541 aatatataca gctgccccga gaatataaca tttcaaacct tagccagaat aaaagaaata
      601 tgccctgaat gtgaattagg agaaaaacta tttattactg gaacaaattt gagtacccaa
      661 aaacatgaag tgttttccat tgatacaaca ccatctatgg cactcgccga tgccattatt
      721 atttccgcaa atttacctat tgcctttgag cgtatctgct atcaaggaaa cgtatacagt
      781 gatggaggaa tcagcaataa tttacctgcc cattgttttt cagaaaaagg gcataaaaca
      841 acctttttga aacacaagga tgatgttgac ttcagtgtac ttgctctgca gtttgataat
      901 gggcttgaag agaacgctct ttatagtcag aacccaatac ctaaatggtc atggctaagc
      961 aacacattct actcattaat tacaggacat ccaaatgtta ctgaaaactg gcatgaggat
     1021 cttcaaatac tgaggcgaca tgcccatcaa agtattttaa ttaaaacgcc aactattgct
     1081 ttgaccaatc tcacaattag ccaagataca aaaaaagccc tggtcgaatc agggcgaaca
     1141 gctgccaaga actatctgga tctccatgag ttttgtatgg atgattatgg aaatatcaga
     1201 cataatgaat gcttgcatga aaaatttcaa aaacccgaag agttattgga ttattgtgtc
     1261 ctacacagcc actttgaact tttaaaaaaa ataaagcaag
//
LOCUS       DASLOE010000312         1298 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.314, whole genome shotgun sequence.
ACCESSION   DASLOE010000312 DASLOE010000000
VERSION     DASLOE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.314"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..1147)
                     /locus_tag="U6309_002990"
     CDS             complement(<1..1147)
                     /locus_tag="U6309_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506391.1"
                     /translation="MGGLTSEQYHSQVVGKIGYIARCMQTIDPENNLKKIREDYQDVL
                     IWAEKNYRFEEILEASKSGKCPNDLDALSRRSLILQELLRLVSSISPFKMKLDLIESQ
                     YEKMKQHVNLWKSDYHVKLNQLNQLTDYLKNAAPTPKNHFLRAMTSALQMQIAQYGIT
                     EDNEGINQLFKLGLHLLAMANEKIDEQYHLFKRYVKDQPEESPFEGILPVEDQKILVK
                     AMIDYAVPKLSLKVLQDKLSALSSSDALTKTLLDSIDRIVEENEKLNALSKVKLGKFS
                     LDIREIEEIYSQALKISPQDALLYTAQQCDAKLLSMAFPDSQNYIVESISNKEAKAIA
                     ELIHSKEFLYQIIKTEVLKQVDPNEKIRLQAAIELYQLLGRTMDKQIH"
BASE COUNT          370 a          225 c          210 g          493 t
ORIGIN      
        1 gatggatctg tttatccatg gttctcccaa gaagctgata caattctatt gcggcttgta
       61 gtcgaatttt ctcattagga tcaacttgtt ttaaaacttc tgttttaata atttgataaa
      121 gaaattcttt actatggatt agctctgcaa tggccttggc ctctttgttc gatatagatt
      181 caacaatgta attttgggaa tcaggaaatg ccatgcttaa taatttggca tcacattgtt
      241 gagcagtata tagcaaagca tcttgtggtg atattttcag agcttgagag tatatctctt
      301 caatttccct gatatccagg ctaaatttac ctaatttgac tttgcttaaa gcatttaatt
      361 tttcgttttc ttcaacaatt ctgtcgatgc tatcaagtaa tgtttttgtt aatgcatcgg
      421 aactggatag tgctgataat ttatcttgta aaaccttaag tgaaagtttc ggcacggcat
      481 aatctatcat agctttgact aaaattttct ggtcttccac aggcaaaatg ccctcaaaag
      541 gagattcttc tggctgatct tttacatacc ttttaaacag atgatattgt tcatctattt
      601 tttcgttagc cattgcgagt aaatgcaatc ccagtttaaa caactggttg ataccctcat
      661 tatcttctgt aatcccatat tgggctatct gcatttgcaa tgcagaggtc attgctctta
      721 gaaaatgatt tttaggagtt ggtgctgcat ttttgaggta gtctgttaat tgattcaatt
      781 gatttagttt tacatggtaa tcacttttcc ataaattcac atgttgtttc attttctcat
      841 attgagactc aatcaggtct aatttcattt taaagggact aattgatgaa acaagccgta
      901 gcaattcttg gagtattaac gatcgtcgac ttaatgcatc cagatcattt gggcactttc
      961 cagatttgct ggcttccaat atttcttcaa aacgataatt tttctctgcc catatcaata
     1021 catcttggta atcttctctt atctttttca aattattttc aggatctata gtttgcatgc
     1081 atctggcgat atatccaatt ttgccgacta cttgactatg atattgctcg ctagttaagc
     1141 cacccatttt tatacccacc ttgataaaag ataattaatt gcccaaatag tatcaataat
     1201 gggtaaaaaa ctcaaggtgt tgccatgtaa aagtttccat ttagtaaatt atgtatgaat
     1261 tttgttaaga aaaattactg ttttcaaata tttaattc
//
LOCUS       DASLOE010000313         1290 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.315, whole genome shotgun sequence.
ACCESSION   DASLOE010000313 DASLOE010000000
VERSION     DASLOE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.315"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            172..813
                     /locus_tag="U6309_002991"
     CDS             172..813
                     /locus_tag="U6309_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN5506392.1"
                     /translation="MKTLVITGISRGIGLEIARIFLDKDWTVIGTSTSGYSPIKHKNL
                     RTYKLDLAISEHIDAFAKNLPKIDVLINNAAVLLEEWNEEKINMSLLKKTFAINLFGT
                     VELTEKCIPKFNDGAQIINISSGWGAFSSNNSPFQPHYKMSKVSLNMYTKLLAERLPQ
                     ITVSSFDPGWVKTNMGTQNAKKLPSETALEIYELISMKKKSGYFWFNGKPRDW"
     gene            871..>1290
                     /locus_tag="U6309_002992"
     CDS             871..>1290
                     /locus_tag="U6309_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506393.1"
                     /translation="MSFDFIKSIWIAALIGITPTLSYSESNIQYQETLQSHCFETWMK
                     KMDPSIDKETYKQFGDKYCRCISTKNLDNKTGVQKAIRNCIPQTILHDAMDELEEEVT
                     LSEVTTQTIEQYCLNRWSLIYPNQSDEDKKTIQAYCAC"
BASE COUNT          488 a          203 c          231 g          368 t
ORIGIN      
        1 cttggggctt aagaaaaatc tcaagatcca ggtaaaaaac gttttttagg agggaattta
       61 gataaactaa atgattgaat tataaacatt ttttaacgaa gagattgagt tttgcaaaaa
      121 gttaaaagat caattatttt gattttataa gccacggata gaggatttaa attgaagact
      181 ctcgtcataa caggaatcag tagaggtata ggcctggaaa ttgctagaat tttccttgac
      241 aaagactgga cggttattgg tacttccacc agcggatatt ctcctataaa acataaaaac
      301 ctacgcacat acaagttaga cctagccatt tcggagcaca tcgatgcatt tgcaaaaaat
      361 ttacctaaaa ttgatgtact gattaataat gccgccgtgt tgcttgagga gtggaatgaa
      421 gaaaaaatta atatgtcctt attgaaaaaa acctttgcaa ttaacctgtt tggtacagtt
      481 gagttaactg aaaaatgtat ccccaagttc aatgatggtg cacaaatcat taatatctct
      541 tcaggatggg gagcatttag ttccaataat tcgccctttc aaccacatta taaaatgtca
      601 aaagtcagtt tgaatatgta tactaaattg cttgcggaaa gactccccca aataactgta
      661 tccagttttg atccaggttg ggttaaaaca aacatgggaa cacaaaatgc aaaaaaactt
      721 cccagtgaaa cggcactgga aatctatgag ctgattagta tgaagaagaa aagtggttat
      781 ttttggttta atggaaaacc tagggattgg tagctaatta aatttcagtt attctcaact
      841 ataaaataaa gaatcaaagg agtgacaaac atgagtttcg attttataaa atcaatatgg
      901 attgcagctc ttataggtat aacaccaacc ttgtcttatt cagagtctaa tattcagtat
      961 caagaaacac ttcaatcaca ttgctttgaa acctggatga aaaaaatgga tccatcaatt
     1021 gacaaagaaa catataaaca atttggagat aaatattgtc gatgcatttc aactaaaaat
     1081 ctcgataata aaacaggggt tcaaaaagca attagaaatt gtataccaca aacaatttta
     1141 catgatgcca tggatgaact ggaggaagag gtgactttat cagaagtaac aacacaaaca
     1201 atcgaacaat attgtcttaa tagatggagc cttatttatc ccaaccaaag cgatgaagat
     1261 aaaaaaacaa tccaggccta ttgtgcgtgt
//
LOCUS       DASLOE010000314         1287 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.316, whole genome shotgun sequence.
ACCESSION   DASLOE010000314 DASLOE010000000
VERSION     DASLOE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.316"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            29..940
                     /locus_tag="U6309_002993"
     CDS             29..940
                     /locus_tag="U6309_002993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506394.1"
                     /translation="MSKCLNAVYFDESGNTEQGFKDKSQPCITFASHNFSENNCKIIL
                     KSHFPGNQAKEVKYSNLGKKGRVDKCFNVLTELKKREGSCFKAYSVYKPLYLFHSFLT
                     LFHDSAYEEKYPNKSHITGDPDWTFSRMHDLWFHFIRIKGLDYILKLIELYSSLIKEG
                     SEKGFESLIKHIEILTNECEHAALIYNWILNTTFEGTVKLINGESFQDEFIKPSWLDT
                     TLPCMIQLLNHWTYFFDGKNFDVVHDASWVLKKREWVIDKLKGITKDKPRILNSKLQD
                     KCFMFFLITYQYIALNLKIQKIMLRCK"
BASE COUNT          459 a          161 c          226 g          441 t
ORIGIN      
        1 cacaaataag gaaatataac gaatgataat gagcaaatgt ctaaatgctg tttattttga
       61 tgaatcaggt aatactgagc agggattcaa agataagagt caaccttgta ttacatttgc
      121 ttcacacaat ttttcagaaa ataattgcaa aataattttg aaaagccact tccctggaaa
      181 tcaagcgaaa gaagttaaat attcaaatct tggtaaaaaa ggacgagttg ataaatgttt
      241 taatgtacta actgaattaa aaaaaagaga gggctcttgt tttaaagctt attcagttta
      301 taaacctctt tatctctttc attccttttt aactttattt cacgatagcg cctatgaaga
      361 aaaataccct aacaaatcgc atattacggg tgatccggat tggactttct ctaggatgca
      421 cgatttatgg tttcatttta ttagaattaa aggattagac tatatattga aattgattga
      481 gctgtattca tctttgataa aagaggggtc ggagaagggg tttgaatcgt taattaaaca
      541 tattgaaatt ttaacaaatg aatgtgagca tgcagcttta atttataact ggattttaaa
      601 tacgactttt gagggcactg ttaaattaat aaatggtgaa tcatttcaag atgaatttat
      661 caaaccttca tggttagata caactttacc ctgcatgatt cagttactaa atcattggac
      721 atattttttt gatggtaaaa attttgatgt agtccatgat gcttcttggg tattaaaaaa
      781 aagagaatgg gttattgata aattaaaagg gatcacaaag gataagccaa ggatattaaa
      841 ttcaaaattg caagataagt gctttatgtt tttcctgatt acttaccaat acatagcttt
      901 gaatttgaaa attcagaaaa ttatgctgcg ttgcaaatag cagacttgtt ggccggatta
      961 ataaatgaat ttatgattga tttgtgttct ggaaaagctt ctgatcagca ttttggtatt
     1021 attagagacc ttattaccga agatagtttt atatatatgt ttccaattat tgattatgct
     1081 cgagaagaag ataagcataa taatatgcag taaaaacgtg caatatatgt gatgacaaac
     1141 ttaatagcta aggagcctgt ttttataggg aatcccaaaa tcccatgttt ttctgtatta
     1201 aattgttata agcagcgtct gcttgggtgt taaaaaacaa tttaatagaa gagtaatatt
     1261 agtaaattca tctgtctata atatgat
//
LOCUS       DASLOE010000315         1286 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.317, whole genome shotgun sequence.
ACCESSION   DASLOE010000315 DASLOE010000000
VERSION     DASLOE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.317"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..560)
                     /locus_tag="U6309_002994"
     CDS             complement(<1..560)
                     /locus_tag="U6309_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN5506395.1"
                     /translation="MNISNTKERILAVAEALIQKDGYNAFSFKDIATAINIKTASIHY
                     HFPSKEDLGVAVISWHADKIAAVLSDISNNSSLSAKEKIQKFFDAILTITYNAENKMC
                     LGGMFASDFQSLPVSIQNQAKKFFELIIEWLKGVLKANGYDNESSLSIAKQIISLIEG
                     GLLLARLYGDETFLEGVRRFIDQTIK"
     gene            826..1053
                     /locus_tag="U6309_002995"
     CDS             826..1053
                     /locus_tag="U6309_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506396.1"
                     /translation="MFFKRSNPHVTPQDLQKVIQNLNAQRELTERQLKEGSISQKTGQ
                     EEMQRLSSLIGAYQNNLMAALDDQKNTNCPK"
BASE COUNT          424 a          212 c          221 g          429 t
ORIGIN      
        1 cactttattg tttgatcaat aaagcgccga actccttcca ggaaggtttc atctccatat
       61 aatcttgcta ataataaccc gccttcaatc aaggaaataa tttgttttgc gatagataat
      121 gaggattcat tatcataacc atttgcttta agaactcctt taagccattc aatgataagt
      181 tcaaaaaatt tttttgcctg attttgaatt gaaacgggta atgattgaaa atcagaagca
      241 aacatacctc cgaggcacat tttgttttca gcgttatagg tgattgttaa aatggcatca
      301 aagaattttt gaattttttc cttggctgat aacgacgaat tattgcttat gtcagataac
      361 acagcagcaa ttttatcggc atgccaggat ataactgcca caccaagatc ttcttttgaa
      421 ggaaaatgat aatggatgct ggcagtttta atattgatgg ctgttgcaat atctttgaaa
      481 ctaaaggcgt tatatccgtc tttttggatc aatgcttctg caactgctaa tatccgttct
      541 ttcgtattac ttatattcat agggacgaat tttacctact catagataga ttgtcaaact
      601 ttttatagtt ttctatatca aattaaaata acacttgttt atctaaatag aagtggatgg
      661 tggactgctc attcattaat tcattctgat ctttattggg ttgatttatc ggatcaatat
      721 tttggtacat cgccatgtca attggcctta aagactttat gtctttattg atctcgaaaa
      781 aaacgggagg taatatatgt gcattagttc acaatagagg tcattatgtt ttttaaaaga
      841 tcaaaccctc acgtaacacc acaagattta caaaaagtga ttcaaaactt aaatgcgcaa
      901 cgtgaattaa cagagcgtca attgaaagag ggttctatat cacaaaaaac agggcaagaa
      961 gagatgcaac gtttatcaag cctgattgga gcatatcaga ataatctaat ggcggcatta
     1021 gacgatcaga aaaacactaa ttgccctaag tagctaatct ttttgaggta taaaagggca
     1081 gtcattttga ttaatggcat aatcaactca taatctgaat ggtcattgat gtgttgatac
     1141 gattttgaga gctctatgcc atcctataca ctaagaaaac agggatgtta tttcttaccc
     1201 tatcaattca tccaaatact cactagcgct ttgctttgaa taatattcag gagtctattt
     1261 aagagattgg aaataaaata gattat
//
LOCUS       DASLOE010000316         1216 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.318, whole genome shotgun sequence.
ACCESSION   DASLOE010000316 DASLOE010000000
VERSION     DASLOE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.318"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            32..>1216
                     /locus_tag="U6309_002996"
     CDS             32..>1216
                     /locus_tag="U6309_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEN5506397.1"
                     /translation="MAMRIFVMILLFFFSISPTLGDSTLPAIFDVKNANKQFDQINLQ
                     LSVQNLNLNNLNKAVETLSELSAQSDECIADIQKKINGVDVLIKQATSTGNDKGADLV
                     YLNNQKHQLAELQSQCRLFSIRAQEAIEAYKSAILQLRQEKTLARTLPLWQLASKIIN
                     SSDNTKWINHTKEKFLLQDLPVYLWFCIVAGSFFISILILSKLRQSNVIRRYVKLKKI
                     RWSHITLLALFMMTGSFYGYISLFTGEEQLINSQFLLIKELFWFLCGTLFIVCLFKVK
                     KIKAFFDWYAMDSEFFEILSIVIVAFYFLALSEQRLFDIWEVNKIIVELTRSIFLFLL
                     LGTTAYFTHYFCRSHLHISFVKRHYNFIRYFIFALLSGCAILNINGFYTLAMQLTLSG
                     IFT"
BASE COUNT          367 a          191 c          206 g          452 t
ORIGIN      
        1 gttaatagtt tgttaattgg caaggaagag aatggccatg agaatttttg tcatgatttt
       61 gcttttcttt tttagtattt ccccgacttt gggggatagc actttgcctg caatttttga
      121 tgtcaaaaat gcaaataaac aatttgatca aatcaatctc cagttgtcag tacaaaatct
      181 taatttaaat aacctgaata aggcagtaga aactctcagt gaactgtctg cgcaatccga
      241 tgaatgtata gctgatatac agaaaaaaat aaatggagtg gatgtattaa tcaaacaagc
      301 tacctctaca gggaatgata aaggagctga tttagtctat ctgaataatc aaaagcatca
      361 gttagctgag ttacaatctc aatgtcgatt attctcaatt cgagctcagg aagcaataga
      421 agcttataaa agtgctattt tgcaattaag gcaagaaaag actttggcgc gtaccttacc
      481 attatggcaa ttagcaagta aaattattaa ttcatctgat aataccaaat ggataaatca
      541 cactaaagag aaatttttac ttcaggattt gcctgtatat ctatggtttt gtattgttgc
      601 aggaagcttt tttatctcta tcctgatctt gtcaaaatta cggcaatcca atgttatacg
      661 ccgttatgtg aaattaaaaa aaattcgctg gtctcatatt acacttttgg ctttgtttat
      721 gatgacgggt tcattttatg gctacatttc cctatttact ggtgaagagc agctaatcaa
      781 ttctcaattc cttttaataa aggagctatt ttggtttctt tgcggcacat tattcattgt
      841 ttgtttattc aaagtcaaaa aaatcaaagc tttttttgat tggtatgcta tggatagtga
      901 attttttgaa atactaagca ttgtgattgt tgcattttat ttcctagcat tatcagaaca
      961 gagattattt gatatttggg aagtcaataa gattattgtg gagttaactc gctccatctt
     1021 tcttttttta ttgcttggta caaccgctta ttttactcac tatttctgcc gttctcatct
     1081 gcatatttct tttgtaaaaa gacattataa ttttattcgg tactttattt ttgcactgct
     1141 ttcagggtgc gcaattctta atattaatgg cttttatact ttagccatgc agcttactct
     1201 atctggtatt ttcact
//
LOCUS       DASLOE010000317         1202 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.319, whole genome shotgun sequence.
ACCESSION   DASLOE010000317 DASLOE010000000
VERSION     DASLOE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.319"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..169
                     /locus_tag="U6309_002997"
     CDS             <1..169
                     /locus_tag="U6309_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEN5506398.1"
                     /translation="FALILARLGHAYGLIKKEAKKQFKFRIYSVATTFIIMGILAIFN
                     VSKATILYIVI"
     gene            complement(327..965)
                     /locus_tag="U6309_002998"
     CDS             complement(327..965)
                     /locus_tag="U6309_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506399.1"
                     /translation="MFGVLVAYTKFFAINPYDKKSMSIYQSVKKEFEKDLNHIESSYS
                     ELLKNKDFNSALLVEMKELEIRLKYVLFNWFFLFKKDKQDLSKQICQLKVDVLRLKTK
                     FMISHLLLNHDKYNLEHYKFVVKHLHNYLEDLNDKIGLIKGIDVDIYNDLFSEMYEWF
                     EQVYSLLNNRYDEFKEEFSIGDKWDDWSYDNSSSEDDWDDNEPLVFLNNTIP"
BASE COUNT          404 a          219 c          160 g          419 t
ORIGIN      
        1 gtttgctctg attctggcac gcttgggaca tgcttatggt ttaatcaaaa aagaagcaaa
       61 aaaacaattt aaatttcgca tttattcggt tgcaacaacg ttcattatca tgggaatttt
      121 agcgattttt aatgtaagta aagcgacgat actatatatc gttatataat aatttagatg
      181 attttaggtt tttggcgatt tgccccctat aatctgcgga tgaaacaatg gagatactca
      241 tcaaatacat attgacttac gcctgctaca gaagccttac cctactgaat aattctcatt
      301 gagccacatc acgacatatt aattcattaa ggaatcgtat tgtttaagaa aaccaggggc
      361 tcattatcgt cccaatcatc ctcgctacta ctattgtcat agctccaatc atcccattta
      421 tcgcctatag aaaactcctc tttaaactca tcatatcgat tgtttaatag agaatatact
      481 tgctcaaacc attcatacat ctcgctgaac agatcgttgt aaatatcaac gtcaatacct
      541 ttaatcaggc ctatcttatc attgagatct tccaggtaat tatgtaaatg cttaaccaca
      601 aatttgtagt gctcaaggtt gtatttatca tgattcaaca gaagatggct tatcataaat
      661 tttgttttta atctcaatac atctactttt aactgacaaa tttgcttgct caaatcttgc
      721 ttatcctttt tgaataagaa gaaccaatta aaaaggacat actttaacct gatctccagt
      781 tctttcattt ctactagcaa cgccgaattg aaatctttat ttttgagtag ttcactataa
      841 gaggactcga tatggtttaa atccttctcg aattctttct taaccgattg gtaaatactc
      901 atacttttct tatcatacgg attgatagca aaaaattttg tgtaagccac taaaactcca
      961 aacattaaag aattaaaaat gccctaataa tataatacaa acaacagatt ggcacttaat
     1021 ttttcaattt gattgtttaa agcaattatt ttatttagca aattggttta tataattcat
     1081 aagtgattct tttaataaag actacaagag tagtaaaaaa gtatttaact tgatgaacac
     1141 tttttatgtt atctgaataa taaattcata acccccactt ttcatataat ctgacgagcc
     1201 ca
//
LOCUS       DASLOE010000318         1191 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.320, whole genome shotgun sequence.
ACCESSION   DASLOE010000318 DASLOE010000000
VERSION     DASLOE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.320"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>1191
                     /locus_tag="U6309_002999"
     CDS             <1..>1191
                     /locus_tag="U6309_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEN5506400.1"
                     /translation="KERLLKTINYHDIKLMIHKQLTNRREAILRINKILKNPDYQQAF
                     ELKLKNNTYYHCYTQPLKLDDEIIGRVWSFRDITIPKRLEMELSHQSTHDMLTNLPNK
                     VLAINILGYAMKTAVSSKKIIGVYLLDLDRFTQINDVFGHSKGDEIIKAISKRLIDCM
                     PMNHVLGRLSGDQFIVIASINNSKEVVTGVTRLMSAFHEPVKMNGHCIKMSCSVGISF
                     YPKDGYTVDTLLSKADIALSRTKIEGRNSFQFYTQEMHSYTLEHILLESELRNAVAKN
                     EFVLYYQPILKLSSLNVIGFEALIRWNHPKKGLILPDKFIPLAEEIGIIGEIGDWVFV
                     TVCKQIRAWQLQGFNNLKISINISPKQFKFKHLPKRINELLSEFQLKPEFIELELTEN
                     VLVDK"
BASE COUNT          439 a          175 c          218 g          359 t
ORIGIN      
        1 aaagagagac ttctgaaaac gataaattat catgatataa agctgatgat tcataaacaa
       61 ttaaccaatc gaagggaagc aatcctaaga attaataaga tcttgaaaaa tccagactat
      121 caacaggcat ttgaattaaa acttaaaaat aatacatatt accactgcta tactcaacca
      181 ctaaaattgg atgatgaaat cattgggcgt gtttggagtt ttagggatat tactattcct
      241 aaaaggcttg aaatggagct gtctcaccaa tctacacatg atatgctcac taatttacct
      301 aataaagtat tggctattaa catactggga tatgcaatga aaacagctgt tagtagtaag
      361 aaaataatag gagtgtattt attagattta gatcgattta cacaaatcaa tgatgtattt
      421 gggcacagca agggagatga aataataaaa gctatttcaa aacggttaat tgattgcatg
      481 ccgatgaacc atgtgcttgg acgacttagt ggtgatcagt ttattgtaat tgccagtatt
      541 aacaatagca aagaggttgt tactggggtc acgcgattaa tgagtgcatt tcacgaaccc
      601 gttaaaatga atggtcattg tattaaaatg agttgcagtg tggggatttc gttttaccct
      661 aaagatggat acacagtgga cacactatta tccaaggctg atattgccct atctcgaaca
      721 aaaattgaag gtcgtaatag ttttcaattt tatacacaag aaatgcatag ctatacctta
      781 gaacacattt tactggaaag tgaattaagg aatgctgtgg caaaaaatga atttgtgttg
      841 tattaccaac caattctaaa attgagttct ttgaatgtga ttggttttga agctctaatt
      901 cgctggaatc acccaaaaaa aggattaatt cttcctgaca aatttattcc tcttgcagaa
      961 gaaataggga taattggtga aattggtgat tgggtgttcg tcactgtatg caaacagata
     1021 agagcatggc aactccaagg gtttaacaat ttaaaaattt caatcaacat ttcaccaaaa
     1081 cagtttaaat ttaaacatct tcctaaaaga attaatgaac tattatccga atttcaacta
     1141 aaaccagaat ttatagaatt ggaattgact gaaaatgtat tagtagacaa g
//
LOCUS       DASLOE010000319         1174 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.321, whole genome shotgun sequence.
ACCESSION   DASLOE010000319 DASLOE010000000
VERSION     DASLOE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.321"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..493
                     /locus_tag="U6309_003000"
     CDS             <1..493
                     /locus_tag="U6309_003000"
                     /inference="COORDINATES: protein motif:HMM:NF025126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN5506401.1"
                     /translation="QKIAKKIQKKEFVYHERTDAYTCPQGQQLFPLRRGAASATAPAY
                     TTYATKACQDCPLKKKCTSSKMGRQIKRYKGDELKEALALVMQQPQAQKRLRQRKAIV
                     EPVFSHIRIRQNFNRFKRRGLHGVKVEFTLQIMAYNISRAIARYFSFLIRLIINHIGN
                     FVF"
     gene            complement(744..1127)
                     /locus_tag="U6309_003001"
     CDS             complement(744..1127)
                     /locus_tag="U6309_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3757 domain-containing protein"
                     /protein_id="HEN5506402.1"
                     /translation="MKRYYSTLLVLALAYASLSAYAHTCPDPQTTSLQWGVPPSPWVE
                     NPFSPNSPQGEENTRFVRANILVAGYGQGVTCTYRNSVGDFSILWQVRTKIPARVDYS
                     WIETMGGYVCTRGFNECQFYVADPS"
BASE COUNT          393 a          253 c          221 g          307 t
ORIGIN      
        1 acaaaaaata gctaaaaaaa ttcaaaaaaa agagtttgtt taccatgaga ggacagatgc
       61 ttacacctgt cctcaagggc aacaactctt ccctttaagg cgaggagctg caagtgcaac
      121 agctcctgct tatacaacct atgcaactaa agcatgccag gattgtcctt taaagaaaaa
      181 atgcacatca agtaagatgg gccgtcaaat caaacgatac aagggcgatg agctcaaaga
      241 agcgctcgcc ttggttatgc agcagcctca agcacaaaaa cgactaagac agcgcaaagc
      301 catcgtagaa ccagtcttca gtcacattag aattcgtcaa aactttaatc gctttaagcg
      361 gagagggctt catggcgtaa aagtcgaatt tacactgcaa atcatggctt ataacatcag
      421 tcgagctatc gctcgatatt tttctttcct tattcgattg ataattaacc atattggtaa
      481 ttttgtattc taatgccata agtcatttca aatacaacaa taaatagaca gttagataat
      541 ttccacagcc tcgtaaggaa gcgggtatat taagccgaag gatcgtaaac gttcacgggt
      601 tatgccatct cttgaaaaac aaatgagata aatgctgtca ttgcgaacga agtgaagcaa
      661 tccagaacga tattttgtta gaattctgta ctggattgct tcacttcgtt cgcaatgacg
      721 gaatttacat gccccatgaa catttacgaa ggatcagcca cataaaactg acattcattg
      781 aaaccacgtg tgcagacata ccctcccata gtttctatcc agctataatc aacacgagcg
      841 ggtattttgg tccgcacctg ccataaaata gaaaaatcac ctacggagtt tctataagta
      901 caagtcacac cctgaccata acctgcgact aaaatattag ctcgcacgaa gcgagtattt
      961 tcttctccct gagggctatt tggggaaaaa ggattttcta cccacggtga tggaggcaca
     1021 ccccattgta atgacgtggt ttgaggatca ggacatgtat gcgcgtaagc gctcaaactg
     1081 gcataagcta aagccaaaac taatagtgta gaataatatc ttttcataaa gactccatca
     1141 attctaatcc tgataattcc aatttattct tgat
//
LOCUS       DASLOE010000320         1159 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.322, whole genome shotgun sequence.
ACCESSION   DASLOE010000320 DASLOE010000000
VERSION     DASLOE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.322"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..763)
                     /locus_tag="U6309_003002"
     CDS             complement(<1..763)
                     /locus_tag="U6309_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg2745 family Dot/Icm T4SS effector"
                     /protein_id="HEN5506403.1"
                     /translation="MLVGSIWFIKDRLQTLMLVQDIRRFLDELRESKTILPCDKRLSA
                     ILQEHFSHRESHEELTSNDIQFVLQCFSERWIADSECDYLLYPSQANQVWIKLAHEIE
                     PFTDKNYLQILLPHITNQFDFNNLTPLTETVRLENFYLGYDGKTLYRKRGLCERLLDN
                     QFELSTCRTLKTKQCEVMTIEELTRLYKGKYCNGEFSIDKEKFDNFWDFLYKKAFPRM
                     QSRGEIPLEVLPHLLMLIESYYHLKNSGADFKLFTA"
     gene            complement(933..1048)
                     /gene="rrf"
                     /locus_tag="U6309_003003"
     rRNA            complement(933..1048)
                     /gene="rrf"
                     /locus_tag="U6309_003003"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          393 a          209 c          187 g          370 t
ORIGIN      
        1 ctgctgtaaa taatttaaaa tctgctccag aattttttaa atgataataa ctctcaatta
       61 acataagcaa gtgaggaagt acttctaatg gaatttcacc tcttgattgc attcggggaa
      121 aggctttttt gtatagaaaa tcccaaaaat tatcaaattt ttctttatca atagaaaact
      181 ctccattgca atattttcct ttatataacc tcgttaattc ttcaatagtc attacttcac
      241 actgtttagt ttttagtgtt ctgcaagtgg ataactcaaa ttgattatca agcaaacgtt
      301 cacataatcc tctttttcta tataaagttt ttccatcata tccaagataa aaattttcca
      361 atcgaacggt ttcagtcaat ggagttaaat tattaaaatc aaactgatta gttatgtggg
      421 gcagcaaaat ttgcaaataa tttttatctg tgaatggttc tatctcatga gccaatttga
      481 tccaaacctg gtttgcctga gaaggataaa gcagataatc acactctgag tcagcaatcc
      541 agcgctcgct aaaacattgc aaaacaaatt gaatatcatt ggaggttaac tcttcatgac
      601 tttctctatg ggagaaatgt tcttgtaaaa tagcggataa tcttttatca cagggaagaa
      661 tagttttcga ttccctcaac tcatcaagaa acctacgaat gtcttgtact aacatcaaag
      721 tctgcagcct atccttaata aaccaaattg aaccaacaag cattctctat caatactatt
      781 gaactggaat cacctaaaca atgtcatgct tgctaatata taggaattat tttgatatca
      841 gattgtactt tggtcaatag aggatttaat taatagaaaa aaacatgtga aaataaaaga
      901 agcggcctaa aaagccgcta taaggaataa aaccctggcg atgacctact ttcgcatgag
      961 gaagcctcac actatcattg gcgcggaaat gtttcacttc tgagttcgag atggtatcag
     1021 gtggttccaa atcgctatag tcgccaggaa aactggttta cctgaggctt tgattgctat
     1081 attaagccac aggtttggta aagagtcaga gtttacacga tacgtttcac gtatacaaat
     1141 caatatcaca ttctgaagt
//
LOCUS       DASLOE010000321         1148 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.323, whole genome shotgun sequence.
ACCESSION   DASLOE010000321 DASLOE010000000
VERSION     DASLOE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.323"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..346
                     /locus_tag="U6309_003004"
     CDS             <1..346
                     /locus_tag="U6309_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN5506404.1"
                     /translation="IWIDEVGFKNMMWNLLKNSLEYIEETHKGEISIWLEQGWEKENF
                     NYLHIKDTAKGLNHKEYEKIFDFFYSGRKEGTGLGLAYCKLLMQAAGGDISCKGKKNE
                     FAHFTLKFPKID"
     gene            complement(394..1089)
                     /locus_tag="U6309_003005"
     CDS             complement(394..1089)
                     /locus_tag="U6309_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011216389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEN5506405.1"
                     /translation="MPEETSTNLPKSLSHFLIQSDLDYKNSLSSIPLFDVKRTIFWDD
                     NLRKMFASIFYHLRGHFINFLWYVANFADNEASKQIIIQNIYEEIGIGNRFSHEKLYE
                     RFAKECGVDIHDEIVNETNYIPFAKEFNKAHLSWLAQHDPKDHLCAFAAYERLDNLDY
                     PFLVELAKSMKISQHGMTFFNVHTHVDHFDTTLQLIVPIWEESPQRLIESFHFIQTHQ
                     LGMWEQFSNALFN"
BASE COUNT          422 a          179 c          197 g          350 t
ORIGIN      
        1 catatggatt gatgaagtgg gttttaaaaa tatgatgtgg aatttgctaa aaaatagtct
       61 ggaatatatt gaggaaaccc ataaaggcga aatctctatc tggctagaac aaggatggga
      121 gaaagagaat tttaattacc tccatataaa agatacagct aaaggtttaa atcataaaga
      181 atatgaaaaa atatttgatt tcttctattc tggcagaaaa gaaggaactg gtttaggctt
      241 ggcctattgt aaacttctga tgcaagcagc aggcggtgat atttcctgta aagggaaaaa
      301 aaatgaattc gctcatttca ctttaaaatt ccccaaaatt gattaattcc tatttaacat
      361 aagaaatccc gagatttctt atgttaaaaa atatcaatta aataaggcat tggaaaactg
      421 ttcccacatc cctaactgat gagtttgaat aaaatgaaat gattctatca gacgctgagg
      481 tgattcttcc catatgggca caatcaattg tagagtggta tcaaaatgat caacatgggt
      541 atgtacatta aagaaggtca tgccatgttg agatattttc atcgatttgg caagttcaac
      601 aagaaaagga taatcaagat tatccaagcg ctcataagcc gcaaaagcac acaaatgatc
      661 tttaggatca tgttgcgcca accaggataa atgagcttta ttaaattctt tggcaaaagg
      721 aatgtagttg gtttcattca ctatttcatc atgaatatca acaccgcatt cttttgcaaa
      781 acgttcataa agcttttcat gggaaaaacg attgccaatt ccaatttctt cataaatatt
      841 ttgaataata atttgttttg aagcttcgtt atcagcaaaa ttggcaacgt accacaaaaa
      901 atttataaaa tgccctctta aatgataaaa aattgatgca aacatttttc taaggttgtc
      961 atcccagaaa atagttcgct taacgtcaaa taaaggaatc gaactcaatg agttcttata
     1021 atccaaatca gattgaataa gaaaatggct gagtgatttt ggtaaatttg tgctcgtctc
     1081 ttcaggcatg attcacactt catatgaaat aagttaagtc cagcaagtta attaaacatt
     1141 ataccatc
//
LOCUS       DASLOE010000322         1128 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.324, whole genome shotgun sequence.
ACCESSION   DASLOE010000322 DASLOE010000000
VERSION     DASLOE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.324"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..616)
                     /locus_tag="U6309_003006"
     CDS             complement(<1..616)
                     /locus_tag="U6309_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEN5506406.1"
                     /translation="MIPWNAYVRLLRLNKPIGILLLWYPTAWALWMANQGFPSIDLLM
                     IFLLGTVFMRSAGCVINDIADRHIDRHVARTQFRPLTSGEVSLSEAFILLFILLCASL
                     LLLLKLPINCFYFAVISVLITFVYPFCKRFLNAPQLILGLAFSMGIPMAFIASGKNLN
                     SDFVVLFLINFTWIIAYDTMYAMTDKADDLKIGVKSTAIYFASYD"
     gene            complement(613..1128)
                     /locus_tag="U6309_003007"
     CDS             complement(613..1128)
                     /locus_tag="U6309_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003636980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEN5506407.1"
                     /translation="MLIADTKGPGLLKDWLEYQGSLTDKLKAMTGNAELERLSQNWSI
                     PNWWDKYVLCIQDKSILQREIVMKNQGIVYWYARSVIPQSCYALKPEFFNRLENESIR
                     NLIFDESSVRRLPILCYPVDQLNLEFHWVKKYIGSEYSQMWVRFTELVFQEKSSFYLV
                     EILLPELESLL"
BASE COUNT          405 a          226 c          183 g          314 t
ORIGIN      
        1 tatcataact tgcaaaatat attgcagtcg atttgactcc tatttttaaa tcatcggctt
       61 tgtcagtcat cgcatacatc gtgtcataag cgataatcca ggtaaaatta attaaaaaca
      121 atacaacaaa atcgctattt aaatttttcc ctgaggcaat aaatgccata ggaataccca
      181 tggaaaaagc caaacctaat atcagttgtg gagcattcaa aaatcgttta caaaatgggt
      241 atacaaaagt aattagcacg gaaataaccg caaaataaaa acaattgatt ggaagtttta
      301 ataataaaag caatgaggca caaagtaata taaacaataa aataaatgcc tcagacaagc
      361 tgacctctcc agaagtcaag gggcgaaatt gggttcgggc tacatgccta tcaatatgcc
      421 tgtcagcaat atcgttaata acacaacctg cggaacgcat gaatactgta cctaataaaa
      481 aaatcataag caaatcaata gaaggaaagc cctgatttgc catccaaagt gcccatgcgg
      541 tgggatacca aagtaataaa attcctattg gtttatttaa gcgtaataat ctgacatagg
      601 cattccaggg aatcataata aactttccaa ctccggtaac aagatttcaa ccaaataaaa
      661 agaactcttt tcctgaaata ctagctccgt aaacctgacc cacatctgag agtattcaga
      721 gccaatgtat tttttaaccc aatgaaactc aagatttaat tgatctacag gataacaaag
      781 tattggtaac cgcctcacac tactttcatc aaaaatgaga tttcgtattg attcattttc
      841 caaacggttg aaaaattctg gcttaagcgc ataacacgat tgtgggataa ccgatcgcgc
      901 ataccaatag acaatccctt gatttttcat aacaatttca cgttgaagaa tgcttttgtc
      961 ctgtatacac agaacatact tatcccacca atttgggata gaccagttct gagataaacg
     1021 ttccagctcc gcattaccgg tcattgcttt tagtttatct gttaaggaac cttggtattc
     1081 taaccaatct ttgagtaatc ctggtccttt ggtatcggca atcagcag
//
LOCUS       DASLOE010000323         1119 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.325, whole genome shotgun sequence.
ACCESSION   DASLOE010000323 DASLOE010000000
VERSION     DASLOE010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.325"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            23..682
                     /locus_tag="U6309_003008"
     CDS             23..682
                     /locus_tag="U6309_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506408.1"
                     /translation="MNQYHQTNEATMIKMKSKLPNSSEFEKFLKSGGQLVKQTEPDTK
                     VWFALKGNDNSLMIFDAFYDSKGREAHFAGQVANALKNNSEQLVLEGWENGVLKNIVN
                     SKVLASKLPARSVQVTLANYIEFTANDGKSEQLAALLSNAAEIVDKTEPQTAFWFALQ
                     LNDNTFAIFDAFSDESGQKAHFSGKVALSLKNHSELLIKNGWEKGVLPHIKNLKVITS
                     I"
     gene            complement(739..981)
                     /locus_tag="U6309_003009"
     CDS             complement(739..981)
                     /locus_tag="U6309_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506409.1"
                     /translation="MKTVLILIIFLCSFQIQAACKCNCDPTDNKMCASQNDLEHPCPS
                     VCASPAPGMAPSFTACPVSRVTDPVTGVTRWVNMCN"
BASE COUNT          390 a          211 c          204 g          314 t
ORIGIN      
        1 acatgcaaaa ggagaaacac ctatgaatca atatcatcaa acaaatgaag caaccatgat
       61 aaaaatgaaa agcaaattac caaattcctc tgaatttgaa aaatttttga aatctggcgg
      121 tcaactggta aagcaaactg aacctgatac aaaagtatgg tttgccctta agggtaatga
      181 taattccttg atgatctttg atgcatttta tgactctaaa ggaagagaag cccattttgc
      241 cggtcaagtt gccaatgctc ttaaaaacaa ttctgaacaa ttggttcttg aaggttggga
      301 aaatggcgta ttaaaaaata ttgtaaacag taaagtgctc gcctctaaat tacctgcaag
      361 atcagttcaa gtaactcttg ccaactacat tgaattcact gccaacgatg gtaaatcaga
      421 gcaacttgct gcgttattaa gcaatgccgc cgagatcgtt gataaaactg agcctcaaac
      481 agctttctgg tttgctttac agctgaatga caacacattt gctatttttg atgcgttctc
      541 agatgagtct ggtcagaaag ctcatttttc aggaaaagtc gcactatctt taaaaaatca
      601 ctctgaactc ttgattaaaa atggatggga aaaaggcgtc ttaccccaca ttaaaaacct
      661 gaaagtgata accagcatct aaccagtcag gtaaattaaa gataaaattc tttcctgaaa
      721 ataatggatt atttgatatt aattacacat attgacccat ctggtaactc ctgtgactgg
      781 atcagtaacc cttgatacag ggcatgctgt aaacgaaggc gccataccag gtgctggcga
      841 agcacaaact gaagggcaag gatgctccag atcattctga ctggcacaca tcttattgtc
      901 tgttgggtca cagttgcatt tgcatgccgc ctgtatttga aaagaacata aaaaaataat
      961 taatattaag actgttttca tctggaaatc cttttcaata agggaatgta atttagcatt
     1021 caagcaatga tatcaggtta cccatgtgta caattttagc acattaatta ggctgcaata
     1081 aaaatactgc ctttaaacgg acttacgaaa aaccccaag
//
LOCUS       DASLOE010000324         1115 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.326, whole genome shotgun sequence.
ACCESSION   DASLOE010000324 DASLOE010000000
VERSION     DASLOE010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.326"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1115
                     /locus_tag="U6309_003010"
     rRNA            <1..1115
                     /locus_tag="U6309_003010"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          274 a          244 c          341 g          256 t
ORIGIN      
        1 ggctgcgact gtttattaaa aacacagcac tctgcaaatt cgtaagaaga cgtatagggt
       61 gtgacgcctg cccggtgccg gaaggttaat tgatggggtt atcttcggag aagctcttga
      121 tcgaagcccc ggtaaacggc ggccgtaact ataacggtcc taaggtagcg aaattccttg
      181 tcgggtaagt tccgacctgc acgaatggcg taacgatggc cacactgtct ccacccgaga
      241 ctcagtgaaa ttgaaatcgc tgtgaagatg cagtgtaccc gcggctagac ggaaagaccc
      301 cgtgaacctt tactatagtt ttgcactgga ctttgatgat gactgtgtag gataggtggg
      361 aggctgtgaa gtgaggacgc tagttctcat ggagccgccc ttgaaatacc accctgttgt
      421 tattgaggtt ctaacttggt ccagtaatcc tggatgagga cagtgtatga tgggtagttt
      481 gactggggcg gtctcctccc aaagagtaac ggaggagcac aaaggtaccc tcggtacggt
      541 cggacatcgt accaagagtg taaaggcaaa agggtgcttg actgcgagag tgacggctcg
      601 agcaggaacg aaagttggtc ttagtgatcc ggtggttctg aatggaaggg ccatcgctca
      661 acggataaaa ggtactccgg ggataacagg ctgataccgc ccaagagttc atatcgacgg
      721 cggtgtttgg cacctcgatg tcggctcatc acatcctggg gctgaagcag gtcccaaggg
      781 tatggctgtt cgccatttaa agtggtacgc gagctgggtt tagaacgtcg tgagacagtt
      841 cggtccctat ctgccgtggg cgtaggaaaa ttgagaggag ctgctcctag tacgagagga
      901 ccggagtgga cgaacctctg gtgcaccggt tgtcacgcca gtggcatggc cgggtagcta
      961 agttcggacg ggataaccgc tgaaagcatc taagcgggaa gcctccctca agatgagttt
     1021 tcccatgaag cccgttgaag actacgacgt tgataggcaa ggtgtggaag cgcagtaatg
     1081 cgtgaagcta acttgtacta attggctgat tgtct
//
LOCUS       DASLOE010000325         1113 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.327, whole genome shotgun sequence.
ACCESSION   DASLOE010000325 DASLOE010000000
VERSION     DASLOE010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.327"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..352)
                     /gene="lem24"
                     /locus_tag="U6309_003011"
     CDS             complement(<1..352)
                     /gene="lem24"
                     /locus_tag="U6309_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dot/Icm T4SS effector Lem24"
                     /protein_id="HEN5506410.1"
                     /translation="MWIFKDITDRYYKKILDSYQLQADEKTYKGKEAELLIQRNELAR
                     CLIKTISLMEVKTQEDYANYFKRVSDEINSALQAAKNAVKEYNEEHNTDFTTDLYESF
                     FTYSLSNFIEVVSDL"
     gene            883..>1113
                     /locus_tag="U6309_003012"
     CDS             883..>1113
                     /locus_tag="U6309_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN5506411.1"
                     /translation="MKSKYISLCFFSLILWFGSPILLAQPSRLDNVINQLVQPFIYAH
                     QTPGLAIAIYYNGKDYYYNFGFADRKSKKPVTK"
BASE COUNT          382 a          179 c          172 g          380 t
ORIGIN      
        1 acaagtcaga tacgacttca ataaaattgc ttaaactgta tgtaaaaaaa ctttcataca
       61 aatctgttgt gaaatcagta ttatgttctt cattatattc tttgacagca tttttggctg
      121 cttgtaatgc cgagttaatt tcatcagaga ctcgtttaaa ataattagca taatcctctt
      181 gagttttaac ttccattaat gaaatggtct taatcaaaca tctggcaagc tcatttcgtt
      241 gaattaataa ttccgcttct ttgcctttat atgttttttc atctgcctgc aattggtagc
      301 tatccaaaat ttttttataa tagcggtcgg taatatcttt aaaaatccac attatctacc
      361 tctttaatta gtgatccaga aagtatttgc aaaaattgtc gctgattatc aagaacaata
      421 acgtcttcct tttctattac gggcgatagt caatattgta atgacagcaa acacatgatt
      481 tccttgtctg atgtattggt aatccattga taactgccaa gaattataaa taaatgttca
      541 aaaaacagta ggaatagcca tggtgtctaa tagtaactca ttgtaagtta taggaaaagt
      601 atctgtattc ggttttttgg aaatagttga agacaaaaaa cactagagag aaatatactt
      661 acaaggctta aaaacagtat tgatttacaa agcaattatt tggctctttt ctaaaaaaga
      721 acgataatta atcaagagtg gtgaggttac caatttgcag aactcaaata aggtttttgc
      781 tctggacctc ttacatagcc aggatagaat cgctgcaaca tgacctgatg atgcaaaata
      841 aagaaaagat aattattata ttttcccaaa aagacattaa tcatgaaatc aaaatacatt
      901 agcttatgct ttttttcact cattttatgg tttggcagtc caattctttt ggcacaacca
      961 tccaggctgg ataatgtaat taatcaactg gttcaaccat ttatttatgc acatcagact
     1021 cccggattag ccattgccat ttattataat ggaaaggatt attattataa ttttggattt
     1081 gctgacagga aaagtaagaa gccagtcact aaa
//
LOCUS       DASLOE010000326         1071 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.328, whole genome shotgun sequence.
ACCESSION   DASLOE010000326 DASLOE010000000
VERSION     DASLOE010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.328"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1051
                     /locus_tag="U6309_003013"
     rRNA            <1..1051
                     /locus_tag="U6309_003013"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          277 a          230 c          337 g          227 t
ORIGIN      
        1 gaagaagcac cggctaactc cgtgccagca gccgcggtaa tacggagggt gcgagcgtta
       61 atcggaatta ctgggcgtaa agggtgcgta ggtggttgat taagttatct gtgaaattcc
      121 tgggcttaac ctgggacggt cagataatac tggttgactc gagtatggga gagggtagtg
      181 gaatttccgg tgtagcggtg aaatgcgtag agatcggaag gaacaccagt ggcgaaggcg
      241 gctacctggc ctaatactga cactgaggca cgaaagcgtg gggagcaaac aggattagat
      301 accctggtag tccacgctgt aaacgatgtc aactagctgt tggttatatg aaaataatta
      361 gtggcgcagc aaacgcgata agttgaccgc ctggggagta cggtcgcaag attaaaactc
      421 aaaggaattg acgggggccc gcacaagcgg tggagcatgt ggtttaattc gatgcaacgc
      481 gaagaacctt acctaccctt gacatacagt gaattttgca gagatgcatt agtgccttcg
      541 ggaacactga tacaggtgct gcatggctgt cgtcagctcg tgtcgtgaga tgttgggtta
      601 agtcccgtaa cgagcgcaac ccttatcctt agttgccagc atgtgatggt ggggactcta
      661 aggagactgc cggtgacaaa ccggaggaag gcggggatga cgtcaagtca tcatggccct
      721 tacgggtagg gctacacacg tgctacaatg gccgatacag agggcggcga aggggcgacc
      781 tggagcaaat ccttaaaagt cggtcgtagt ccggattgga gtctgcaact cgactccatg
      841 aagtcggaat cgctagtaat cgcgaatcag catgtcgcgg tgaatacgtt cccgggcctt
      901 gtacacaccg cccgtcacac catgggagtg ggttgcacca gaagtagata gtctaacctt
      961 cggggggacg tttaccacgg tgtggttcat gactggggtg aagtcgtaac aaggtagccg
     1021 taggggaacc tgcggctgga tcacctcctt acatagaaag gcacagaagg a
//
LOCUS       DASLOE010000327         1068 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.329, whole genome shotgun sequence.
ACCESSION   DASLOE010000327 DASLOE010000000
VERSION     DASLOE010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.329"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            126..365
                     /locus_tag="U6309_003014"
     CDS             126..365
                     /locus_tag="U6309_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEN5506412.1"
                     /translation="MDNREKSRLLWKCRRGMLELDLLLQKFIANEIDRLTENQLKAFD
                     NLLTHNDPNLYAWLMGHEEPEKELLEIVSFIRNCD"
     gene            307..750
                     /locus_tag="U6309_003015"
     CDS             307..750
                     /locus_tag="U6309_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lpg1578 family Dot/Icm T4SS effector"
                     /protein_id="HEN5506413.1"
                     /translation="MRNPKKSCLKLYHSSETVIKPGKSKVYLRLIAILYLCTIGLVLY
                     SSLPVLIKGVLIFLILIQFKNDVTYQSACQKVKEIKYLKNLTWSLETIDGTTHHYDKL
                     KILIHNMLFQVIQLSSDKKNQMLILFNDQIPNKQLRLLHLNSNKK"
     gene            851..>1068
                     /locus_tag="U6309_003016"
     CDS             851..>1068
                     /locus_tag="U6309_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN5506414.1"
                     /translation="MHIETSLSNESLIELAQKGNGLAFDKLFERYQGKIRQMIYFYTH
                     DWTNVNDLTQEVLIKVFRHLPYFNERSQ"
BASE COUNT          400 a          161 c          162 g          345 t
ORIGIN      
        1 atcaagagca atgatgttgt ttataataca gactagtgta tcatttggcc tctcaaaatc
       61 agatatcatt attgaataat taggattatg ttgtatttat cgtgaagtaa cagaggaaat
      121 ccattgtgga caatagagaa aaatcaaggt tattatggaa atgcaggcga ggaatgctag
      181 agcttgatct tcttttacag aaattcatag ccaatgagat tgatcgactt acagaaaatc
      241 aattgaaagc atttgataat ctattaaccc acaatgaccc taatttatat gcctggttaa
      301 tggggcatga ggaacccgaa aaagagttgc ttgaaattgt atcattcatc agaaactgtg
      361 attaaaccag gtaaatccaa agtatatctt cgattaattg cgattcttta tttatgcacc
      421 atcggattag ttttatattc ttctttacct gtgttgataa aaggcgtctt aatttttctt
      481 atccttattc aatttaaaaa tgatgtaact taccaaagtg cttgccaaaa agttaaagaa
      541 atcaaatact tgaaaaacct tacgtggtca ctggaaacaa tagatggtac tacccatcat
      601 tatgacaaac taaaaattct tattcataat atgctatttc aagttattca attatcttca
      661 gataaaaaaa atcaaatgct aattctattt aatgatcaaa tacctaacaa acaattacga
      721 ttattacacc taaacagcaa taaaaagtag tatataataa tacataatga ttaaaataaa
      781 cccaaattaa caatggttag actttatctt atcctaacgt aataacaagc aaagtaataa
      841 tgaggaagct atgcatattg agacctcttt aagtaatgaa tctctaattg aattagctca
      901 aaaaggcaac ggtctagctt ttgataaact gtttgagcgt tatcagggca aaattagaca
      961 aatgatttat ttttacacgc atgactggac aaatgtcaat gatttaactc aggaagttct
     1021 tatcaaagta tttaggcatt taccttattt taatgaacgc agtcagtt
//
LOCUS       DASLOE010000328         1066 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.330, whole genome shotgun sequence.
ACCESSION   DASLOE010000328 DASLOE010000000
VERSION     DASLOE010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.330"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            267..872
                     /gene="gstA"
                     /locus_tag="U6309_003017"
     CDS             267..872
                     /gene="gstA"
                     /locus_tag="U6309_003017"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEN5506415.1"
                     /translation="MKLYYCRGACSLVVRIILNELGLNFQDEEVDLKVKKTAGGDDFF
                     KINPKGAVPTLVLDNGDVLTENQVILQYLADTTPGQKLLAAVGESKRYHTLEWLNYIS
                     TELHKSLGMFFNPTVSEEIKSKILTPLVMTRFAYTNTRLAKGSYLMGNEFSLPDAYLF
                     VMVSWAKYFKLDLSQFEHLTKFMDLMLARPSVAKSLQQEKL"
BASE COUNT          324 a          171 c          204 g          367 t
ORIGIN      
        1 ggaatcagct actatgaaat taatcaatga aattgagaaa gatctcaaat gcgccaagtg
       61 cccatattaa atattgttat gataatatca gggttagagc atatcgaatc tttgcttttt
      121 atagagcctg attaaacgtg gtctgattaa ataaaccaca tgcaagatca tcctttttca
      181 ctgtatggtt ttttacattt caagtttttt gctgcatttt atgctaactt agctattatt
      241 atttaaacat catcaaagga gtgaacatga aattatatta ttgcagaggt gcttgttcat
      301 tagtagtccg tattatactc aatgagttag ggttaaactt ccaggatgaa gaggtcgatt
      361 taaaagtcaa aaaaacagca ggtggcgatg atttctttaa aattaatcct aaaggagcgg
      421 tcccaaccct ggttcttgat aacggcgatg tcctgactga aaatcaggtt attctacagt
      481 atctggcaga tactactcca ggacaaaaat tgttggctgc tgttggagag agtaaaagat
      541 atcatactct ggaatggtta aattatattt ctacagagct tcataagtcg cttggtatgt
      601 tttttaatcc tactgtttca gaggaaataa aatccaaaat tttgacacct ttagtcatga
      661 cccgtttcgc ttataccaat actcgtttgg caaaaggatc atatttgatg ggaaatgaat
      721 ttagtttacc tgatgcctat ctgtttgtta tggtaagctg ggcaaaatat ttcaaactgg
      781 atttgtctca gtttgagcat ttgacaaaat ttatggatct tatgttggct cgcccttcag
      841 ttgccaagtc actacagcaa gaaaagttat agcgcgggtt gcgttattaa ttcattgcaa
      901 cccaatttgc ttcttctata tagtactttt taaaaggggg agtaagagtc attatatcca
      961 atgccttgaa gtatgggggt ttaactttct tgttagacag cccatctttt tgggcaaaat
     1021 aaacccttta ggtttaagtt attagcaata atctttgtta ttgtgc
//
LOCUS       DASLOE010000329         1051 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.331, whole genome shotgun sequence.
ACCESSION   DASLOE010000329 DASLOE010000000
VERSION     DASLOE010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.331"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..706
                     /locus_tag="U6309_003018"
     CDS             <1..706
                     /locus_tag="U6309_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN5506416.1"
                     /translation="RNFKSYYLFVLQKNLHPYFPSLVSYSRMTELMSSCLVPLMAFCH
                     HQTKTPTGIYFVDSTPIEVCNVKRASQNKVFKGLAEKSKSTMGWYFGFKLHLIVNDKG
                     ELMAFKITSSRTDDRFVVPNLSENLFGKMIGDKGYISQNLTDKLAERGLQLLTKARKN
                     MKQKVLNTFDKALLRKRAIVESVIDQLKNISNIEHSRHRSIFNFMVNILAGLAAYALK
                     PKKPSLNIQRSFMAVA"
     gene            complement(729..1051)
                     /locus_tag="U6309_003019"
                     /pseudo
     CDS             complement(729..1051)
                     /locus_tag="U6309_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
BASE COUNT          369 a          182 c          180 g          320 t
ORIGIN      
        1 ccgaaatttt aaaagttact acttgttcgt gttgcagaaa aatttacacc cctattttcc
       61 aagcttggtg agttacagca gaatgacaga actaatgtct tcttgtctgg tacccttaat
      121 ggccttttgc caccatcaaa caaaaacacc cactggaatt tactttgtag attcaacacc
      181 aatagaggtg tgcaatgtaa aaagagcaag ccagaacaag gtctttaagg gtttggcaga
      241 gaagtccaag tccacaatgg gatggtattt cggctttaag cttcatttga tagtcaatga
      301 caaaggcgaa ttaatggctt ttaaaataac atcaagccga actgatgacc gatttgtcgt
      361 tcctaatttg agcgaaaatc tgtttggtaa aatgattggc gataaaggtt atatttctca
      421 aaatttaact gacaaattgg ctgagcgagg attacaactc cttactaaag ccaggaaaaa
      481 catgaaacaa aaagtactca atacatttga taaagctttg cttagaaaaa gagccattgt
      541 tgagtcagtt atcgatcaat tgaaaaatat ttccaatatt gaacattcaa ggcatcgctc
      601 tatattcaat tttatggtca atatattggc cggtttagct gcttatgcac ttaaaccaaa
      661 gaagccctct ttaaatattc aacgatcttt tatggctgtt gcttgagcag ttatatcgaa
      721 ttcacgttaa aataattaat tggaataaga gtgtcagacg gaaggctatt atcagcaaat
      781 agaattccaa tataaaaacc atcaataatg atatttttga ctataatatt ttgtctgtta
      841 taagcaacaa tacctctaga taaggtagtt gaggtcccag gcccctttaa aagataacca
      901 ttcccatcta atataacatt attagcctga ataataatcg catcataatc agaaattggg
      961 gtgtacatat tttgatttaa gcaccatttc ccagaatgtt taataacgaa aggaatggaa
     1021 gatatgatat aatcacattt tccaccataa a
//
LOCUS       DASLOE010000330         1031 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.332, whole genome shotgun sequence.
ACCESSION   DASLOE010000330 DASLOE010000000
VERSION     DASLOE010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.332"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            535..>1031
                     /locus_tag="U6309_003020"
     CDS             535..>1031
                     /locus_tag="U6309_003020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506417.1"
                     /translation="MTRNKTEKNIQFDFNECLEKAKANELSSLDILNNKDTWDSLSIK
                     QKQRLCGEWVRHGYFSFEEFNDPDCAPELYGLLNEDIYIVGIKQDPVRLQNIPDDILS
                     TILPVLYEDRNFMDDLIDKACEDITILQSSQELHKKVIELAAHQIWGGKKSIFIRRDT
                     RFYKDV"
BASE COUNT          368 a          167 c          187 g          309 t
ORIGIN      
        1 gtttatttaa gcagacctct ttaaatatct ataataaacc aacctaagca tcaaagttga
       61 aattgatttt agatacggtt tttcttcagg gaagagccag gcctgtttcc gtattttata
      121 tacaaatttc accatggtag aaagaaattt acacccgaaa atttcctgct ttaaccaact
      181 gtcaaaatct cacaatgaga ttgtactcag aaaacaccaa ttgtgttttt aagtatgcag
      241 gttaatggat ccaccacaaa aatcgatttt tcaagagtca tctgcacaag ttgaaaactt
      301 gtatgaatcc cagcaatgta ttactgattg tctggagcaa tcacaacggt taaaacggtt
      361 aaaccaggtg ggtgtcatac acatggcagt cccaacttta acaatatttt attcataatg
      421 cgcaatttac aatactatac taattaagta actattaaaa atacgactaa cgaaaatatc
      481 accttgatga ctttgggttc atctcaagag tagaaaatat tgggtggtta agcaatgacc
      541 agaaataaaa cagaaaaaaa tattcaattt gactttaatg aatgtttgga aaaagccaaa
      601 gccaacgaat tgtcttcttt ggacatttta aataataagg acacgtggga tagtttatct
      661 atcaaacaaa aacaacggtt atgtggggag tgggtgcggc atggatactt cagttttgaa
      721 gaatttaacg acccggattg tgcaccagag ctctatggat tgttgaatga agacatatat
      781 attgttggca taaagcaaga tcctgttcgt ttacagaata ttcctgacga cattctatca
      841 acaattttac ccgtcttata tgaagataga aattttatgg atgatcttat tgataaagct
      901 tgtgaagata taacgattct ccaaagctct caagagctgc ataaaaaagt aatagagctt
      961 gcggcacatc agatatgggg agggaaaaaa tctatattca taaggaggga tactcgattt
     1021 tataaagatg t
//
LOCUS       DASLOE010000331         1013 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.333, whole genome shotgun sequence.
ACCESSION   DASLOE010000331 DASLOE010000000
VERSION     DASLOE010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.333"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            157..>1013
                     /locus_tag="U6309_003021"
     CDS             157..>1013
                     /locus_tag="U6309_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506418.1"
                     /translation="MLNLKKTAVAVLALGSSAVFAGTMGPVCTPGNVTVPCERTAWDI
                     GITALYLQPIYDADWGYNGFTQVGGWQHWHDVDHEWDWGFKLEGSYHFNTGNDINVNW
                     YHFDNDSDHWADFANWHNYNNKWDAVNAELGQFVDFSANKKMRFHGGVQYARIEADVN
                     RYLNNFAFNGFNSKFNGFGPRTGLDMNYVFGNGFGVYAKGAAAILVGTSDFYDGINFI
                     NGSKNAIVPELEAKLGADYTYAMAQGDLTLDVGYMWFNYFNAMHNTGVFNGFETDFAA
                     SGPYIGLKYV"
BASE COUNT          283 a          181 c          235 g          314 t
ORIGIN      
        1 ttgataagaa atttcagcaa tactgttgac tttatagaag tccggctgga taatttatcc
       61 aggtacgctc tcgattagag cgaagaatgc gtactgttgc tgatatgcta aattagtaac
      121 gaacggaaat taataataaa aaaagtggag ataagcatgt taaatttgaa aaaaacagcg
      181 gtagctgttc ttgctttagg aagcagtgca gtgttcgctg gtactatggg accagtttgc
      241 accccaggta atgtaactgt tccttgcgaa agaactgcat gggatattgg tatcaccgca
      301 ctatatttgc aaccaatcta tgatgctgat tggggctaca atggtttcac ccaagttggt
      361 ggctggcagc attggcatga tgttgaccat gagtgggatt ggggcttcaa attagaaggt
      421 tcttatcact tcaatactgg taacgacatc aatgtgaact ggtatcattt tgataacgac
      481 agtgatcact gggctgattt tgctaactgg cacaactaca acaacaagtg ggatgcagtt
      541 aatgctgaat taggtcaatt cgtagatttc agcgctaaca agaaaatgcg tttccacggc
      601 ggtgttcaat acgctcgcat tgaagctgat gtgaaccgtt atttaaataa ctttgccttt
      661 aacggattca actccaagtt caatggcttt ggtcctcgca ctggtttaga catgaactat
      721 gtatttggca atggctttgg tgtttatgct aaaggagctg ctgctattct ggttggtacc
      781 agcgatttct acgatggaat caacttcatt aatggttcta aaaatgccat cgttcctgag
      841 ttagaagcta agcttggtgc tgattacact tacgcaatgg ctcaaggcga tttgacttta
      901 gacgttggtt acatgtggtt taactacttc aacgctatgc acaatactgg cgtatttaat
      961 ggatttgaaa ctgatttcgc agcttctggt ccttacattg gcttgaagta tgt
//
LOCUS       DASLOE010000332          996 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.334, whole genome shotgun sequence.
ACCESSION   DASLOE010000332 DASLOE010000000
VERSION     DASLOE010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.334"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            59..>996
                     /locus_tag="U6309_003022"
     CDS             59..>996
                     /locus_tag="U6309_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN5506419.1"
                     /translation="MYTERSIFKLGKEKSGAIQIGKRGLEQHLLEADICQPFYIKELL
                     SKQDWSIFEEPNKKKKRKPYPAQSMVGLILYGLMQGLSSLRDLERLARVDLGCILITD
                     GIFPDFSSIGYFIRRHAEVLNEAFFIQLTRSILKVTRSGVRSLAGDGTIIEATSSRYT
                     LMRHEAIEKKREALKAQLAAHPEEQNLGAAAEKIEQASTIMQGRIEKRKEQRKPIDTV
                     TINPTEPEAVVQPTKKGKTAVPAYKPSVLANKARVVVALDVHPSNEIDSLVSMLDMTN
                     RANYQEKVEELLLDGGYESHGVLEETLKRDISVLCSS"
BASE COUNT          304 a          177 c          237 g          278 t
ORIGIN      
        1 tgctcttgat atagattgaa acctgattaa gatagtgatt ttttgaaaag gatttaaggt
       61 gtatacggag cgttctattt ttaaactagg caaagaaaaa tcaggggcta ttcaaattgg
      121 gaaacgaggg cttgaacaac atttgctaga agcagatata tgtcagcctt tttatataaa
      181 agaattgtta tctaaacagg attggagcat ttttgaagag ccgaataaaa agaaaaaaag
      241 gaagccctat cctgctcaat cgatggtagg attgatttta tatggtctta tgcaaggttt
      301 atcttcctta cgagatttag agcgtttagc gcgagtagac ttgggctgca ttctgataac
      361 ggatggaatt ttccctgatt tttcaagtat tggttatttt attcgtcgcc acgcagaggt
      421 tttaaacgaa gcctttttta tccagttaac ccgctctatt ttgaaagtga ctcgttcagg
      481 ggtccgttca ttagcggggg atggaacgat tattgaggcg acatcctccc gttatacatt
      541 aatgagacac gaggctattg agaagaaacg agaggcatta aaagcccaat tggctgctca
      601 tcctgaagaa caaaacttag gcgcggctgc ggaaaaaata gaacaggcct cgactatcat
      661 gcaaggtcga attgagaaaa ggaaagaaca gcgtaagcct attgatacgg taaccattaa
      721 tccaacagag ccagaggcgg tggtgcaacc gaccaaaaaa ggtaagaccg ctgttcctgc
      781 ttataaacct tccgtgctgg ccaacaaggc cagggtggtt gtggctttgg atgtccatcc
      841 atcgaatgaa atcgattccc ttgtctcgat gctggacatg actaatcgag ctaattacca
      901 agaaaaggtt gaagagttac tgttagatgg aggctatgaa tctcatggtg ttcttgaaga
      961 aaccctgaag cgtgatatca gtgtattatg ctcttc
//
LOCUS       DASLOE010000333          979 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.335, whole genome shotgun sequence.
ACCESSION   DASLOE010000333 DASLOE010000000
VERSION     DASLOE010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.335"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>979
                     /locus_tag="U6309_003023"
     CDS             <1..>979
                     /locus_tag="U6309_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEN5506420.1"
                     /translation="KDFGEEKLDWLRQYRDFKHGIPADDTIARVISALDSEQFTLCFV
                     NWVNELRSKDGREQIAIDGKTLRRSYTGDRTTALHSITAWSKRNGLILAQMRSNGKAN
                     EKASVLEMLDLLELKGAIVTADAMNTEKKIAKKIHDKGGDYVLCVKNNHKKLLNEIKD
                     YFHIMERDHREIWSQNYFEEVDAGHGRIETRTCRQIAISPWVEGYADWQGLQTVIEVT
                     RTREIKEKTGTEVQYYISSLPLEPKQIADAIREHWAVENKAHWVLDVTYKEDDSRVRR
                     DNAAENLALLRRFALNLARLHPQKNSMKGKLKKAMWSDKFRSELLIGQIQ"
BASE COUNT          349 a          170 c          231 g          229 t
ORIGIN      
        1 aaggattttg gagaagaaaa gcttgattgg ctaagacaat atcgggattt taagcatggg
       61 ataccggccg atgataccat agctcgtgta attagtgctc ttgattcaga gcagttcacg
      121 ttgtgttttg taaattgggt gaatgaactt cgttcgaaag atggtcgaga acaaatagcg
      181 atagatggaa aaactctacg ccgctcatat acaggtgacc gcaccacagc cttacatagc
      241 attactgcat ggagtaaacg aaatgggttg atattagctc aaatgcgctc taatggaaaa
      301 gcaaacgaaa aagccagtgt gttagaaatg ctagacctct tggagcttaa gggagcaatt
      361 gttactgccg atgctatgaa tacagaaaag aagatagcta aaaaaataca tgataaaggc
      421 ggagactatg tgctttgtgt taaaaacaac cataaaaaac tgctaaatga aattaaggat
      481 tattttcata tcatggaaag agaccatcga gaaatctggt cacaaaatta cttcgaagaa
      541 gtggacgcag gccatgggcg catagaaaca agaacatgcc ggcaaatcgc aattagccca
      601 tgggttgaag gttatgccga ctggcaagga ttacaaaccg taattgaagt aacgcgaaca
      661 cgtgaaataa aagaaaaaac cggtactgaa gtgcaatact atatcagttc cttgccgtta
      721 gaacccaaac aaattgcaga tgctatcaga gagcattggg cagtcgaaaa caaagctcac
      781 tgggtgttgg atgtgaccta taaagaagat gactcaagag tacgaagaga caacgcagca
      841 gaaaatctgg ccttgcttcg taggtttgca ctcaatctgg ctcgacttca tccccagaaa
      901 aatagcatga agggaaaatt aaaaaaggct atgtggtctg ataaatttag aagtgaactc
      961 ttgattgggc aaattcagg
//
LOCUS       DASLOE010000334          979 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.336, whole genome shotgun sequence.
ACCESSION   DASLOE010000334 DASLOE010000000
VERSION     DASLOE010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.336"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..175
                     /locus_tag="U6309_003024"
     CDS             <1..175
                     /locus_tag="U6309_003024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506421.1"
                     /translation="DSIKACEKAPTSLVGTLRYSISSILRDTDRVPLHIPRHRITLLG
                     INSTPPRPIPVPD"
     gene            complement(185..>979)
                     /locus_tag="U6309_003025"
     CDS             complement(185..>979)
                     /locus_tag="U6309_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEN5506422.1"
                     /translation="KHFIRYGKKIFILTNSEYSYSKLLLDYALSPFLDKGEHWQGLFE
                     FVITLANKPRFFYDNLRFLSVNPENGTMTNVHGPIVPGVYQGGNAKKFTEDLGVGGDE
                     ILYIGDHIYGDILRLKKDCNWRTALVVEELGEEIASQIRALPIEKKIVEAMAIKKELE
                     QKYVDLCTRSIDESSQQYDQEIHDLQLQISTVDLQISRLLQEQNSFYNPKWERVFRAG
                     AEESYFAYQVDRFACIYMEKLSDLLEHSPMTYFRANRRLLAHDIDI"
BASE COUNT          294 a          213 c          172 g          300 t
ORIGIN      
        1 agacagcatc aaggcctgtg aaaaagcacc aaccagtctg gttggcacac ttcgttatag
       61 catatccagt attttgcgtg atacagatcg tgttccctta cacataccac gtcatagaat
      121 aactttgctg gggattaata gcactccccc tcgtccgatc ccggtacctg attaatagaa
      181 aaaatcaaat atctatatcg tgagctaaca atcgcctgtt ggcacgaaaa taggtcattg
      241 gagaatgctc gagtaaatcg gataattttt ccatgtagat acaggcgaat ctatcgactt
      301 ggtaggcgaa gtagctctct tccgccccag cgcgaaaaac tctttcccac ttgggattat
      361 agaaactgtt ttgttcttgt aacaatcttg aaatttgtaa gtcaacagtg gatatttgca
      421 attgcaagtc atgaatttcc tgatcatact gttgagagct ttcgtcaata cttcttgtgc
      481 aaagatctac gtatttctgc tctagctctt ttttaatagc catggcttca acaattttct
      541 tctcaattgg caaagccctt atttgggatg caatttcttc tcctaattct tcaacaacca
      601 aagcagtacg ccaattacaa tcttttttca atcgtaatat atcaccgtaa atatgatccc
      661 ctatatagag aatttcatct ccgccgaccc cgagatcttc agtaaatttt ttggcattac
      721 ctccttggta tacgccagga actattgggc catggacatt agtcatcgtc ccgttttcgg
      781 gattaactga taaaaatcgt aagttatcat aaaagaaacg gggtttattt gccaaggtga
      841 tgacaaactc aaataaacct tgccagtgct cccctttatc caggaatggg gacaaggcat
      901 aatccaacaa caatttagaa taagaatatt ctgaattggt cagaataaat atctttttcc
      961 cataacgaat aaaatgctt
//
LOCUS       DASLOE010000335          971 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.337, whole genome shotgun sequence.
ACCESSION   DASLOE010000335 DASLOE010000000
VERSION     DASLOE010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.337"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(172..858)
                     /locus_tag="U6309_003026"
     CDS             complement(172..858)
                     /locus_tag="U6309_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEN5506423.1"
                     /translation="MSKFIFVIGGTGTGKTTVSEEIKTIAEAQNFTVTILSLDHYYLP
                     QSMMEPDKPKNFDVPEALEQTLIQQHLQDLEAGKTIFRPTYDMASSDRVLNGEMKIIP
                     ADIIIVEGIFAGEYLSSLRNQTEKLKIYLQSNQINDNYTRKEDRDIVERKRSSAHAHV
                     AKKNQMTGFFRYVATHMISSNMVIDNPWLPQQNMTTTEKLPLIAGNNLIKLNEFLSST
                     ESHFLYKTYS"
BASE COUNT          306 a          172 c          153 g          340 t
ORIGIN      
        1 caaaatcaaa ccattatgat gtagaagcat tacaagatac ttatcaagaa ttggaagatc
       61 tctatgaaga gtattgtaat cctaacaagg aatcactgag cttctaactc aattttaaat
      121 attgcatttt atattgcccc taaattatcc actggggcaa taagttacat tttaactata
      181 tgttttatat aaaaaatgac tctcagttga agataaaaat tcatttaatt ttattaagtt
      241 atttcctgca atcaaaggaa gtttttcggt agtagtcatg ttttgttgag gaagccaagg
      301 attatcaatg accatatttg aagaaatcat atgggtagcg acatatctaa aaaatcctgt
      361 catttgattc tttttcgcaa catgggcatg agctgatgat ctttttcttt caactatatc
      421 tctgtcttcc ttccttgtgt aattgtcatt tatttgattg gattgtaaat aaattttgag
      481 tttttctgtt tgattcctta aagaggataa atactcacca gcaaaaattc cttcgacaat
      541 aatgatatca gcaggaataa ttttcatttc cccattcaat accctgtcac tgcttgccat
      601 atcataggtc ggtctgaata tggttttacc agcctctaaa tcctgcaaat gctgttgaat
      661 taatgtttgt tccagagctt cagggacatc aaaattcttt ggtttgtccg gttccatcat
      721 actttggggc aagtagtaat gatccagact taatatcgta accgtgaaat tttgtgcttc
      781 tgctattgtc ttaatttctt ctgaaacagt cgtttttcca gttccagttc ctccaatgac
      841 aaagataaat ttagacacgt aatattctct tgcggtcaaa aaaggcatta taataactga
      901 ggaacaaatt acaagagaac ctatcaatct gtaccttgat aagctctcga gccaaatcca
      961 gtactctaaa g
//
LOCUS       DASLOE010000336          964 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.338, whole genome shotgun sequence.
ACCESSION   DASLOE010000336 DASLOE010000000
VERSION     DASLOE010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.338"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..645)
                     /locus_tag="U6309_003027"
     CDS             complement(<1..645)
                     /locus_tag="U6309_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506424.1"
                     /translation="MEFCRNFTRVCWDEIREEVKRSNPEFFFLVDEIAPGKDFPLYVF
                     NFGYGDLIGDSETFYIPHQGKLQTLNNFSSQEPITKDLFYGFHSCPLGLVLDKCFEWY
                     LRGDSENETHPVYIDKKGDFFNIGHVTQIKPLKRYLPNGILSVKAGAQTTLVIQNIGC
                     KRSHNRLIRSGIDCTIPNSYHDHSEFFKRIYQSCPAESKWRASIVYFSEKWIDNI"
     gene            806..>964
                     /locus_tag="U6309_003028"
     CDS             806..>964
                     /locus_tag="U6309_003028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506425.1"
                     /translation="MKIVSEQVINGERIATQVIDTILSSTEHNLVTLSDIFGISLNKL
                     VLKKGFSND"
BASE COUNT          322 a          151 c          162 g          329 t
ORIGIN      
        1 aatgttatct atccattttt cagagaagta aacaatagag gccctccatt tactttcagc
       61 agggcaagat tggtaaattc gtttaaaaaa ttcactgtga tcatggtatg aattggggat
      121 ggtgcaatca attccagatc gaataagacg attatggctt cttttgcatc caatattttg
      181 tattacaaga gtggtttgtg ccccagcttt tacagacagt atgccattag gcaagtatcg
      241 tttaagtggt tttatctggg ttacatgtcc aatgttaaaa aaatcccctt tcttatctat
      301 gtaaacagga tgcgtttcat tttctgaatc acctcttaag taccattcaa agcacttatc
      361 taatacaaga cctaatggac aactgtgaaa tccataaaag aggtctttag taattggttc
      421 ttgagatgaa aaattattaa gagtttgaag ctttccttgg tgcggaatat aaaaggtttc
      481 agaatcacca attaaatcac catatccaaa gttaaaaaca tatagtggga aatcttttcc
      541 tggcgcaatt tcatcaacta agaaaaaaaa ctcaggatta gagcgtttta cttcctctct
      601 aatttcatcc caacaaactc tagtaaaatt cctacaaaat tccatattag tctttttgtt
      661 aattaatttg ctttttattt tgaattaaaa tgcgtatttg ccaaagattt tagtgatata
      721 aatgacaaac atattatgct ctaatcaaat caagcttgtc aaataagtaa agtacgttgt
      781 tgttgtcgta ataaggattt taatcatgaa gattgtaagt gaacaggtta ttaatggaga
      841 gcgtattgcg actcaggtta ttgatacaat cttaagcagt acagaacaca atcttgttac
      901 attatccgat atatttggga tatctttaaa taagcttgtt ctgaaaaagg ggttttcaaa
      961 tgat
//
LOCUS       DASLOE010000337          942 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.339, whole genome shotgun sequence.
ACCESSION   DASLOE010000337 DASLOE010000000
VERSION     DASLOE010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.339"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            169..837
                     /locus_tag="U6309_003029"
     CDS             169..837
                     /locus_tag="U6309_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN5506426.1"
                     /translation="MIDTIIFDFDGVILNSEPMHFEALVQVLNQSGINLAYEEYMTHY
                     LGLSDISVFPKILNDKGLAFSSTEIRQLIERKVTVYNELIEDSEQLPMTLDLDWFLVR
                     VAKQYGKIGICSGSNRHSIIKILEKIHCGRLACYFKTIVSCEDVSLGKPSPEGYLLAA
                     HRLQSNPENCLVIEDSEHGVAAAKAGGMLVAGLLTTLSRDQLANADMIVHDFKELAHL
                     LDSQ"
BASE COUNT          310 a          154 c          194 g          284 t
ORIGIN      
        1 aaatttccat acctcttgtg attaagcagg ccaatacaag caattttaac aatagttgtc
       61 taaaataaaa tgaaatattc cttttatcta gcattcagca taaaagtaat ttctcttgtg
      121 taattgacaa tatatatgtt taacaatgtc aatctactga ggcaatacat gatagataca
      181 ataatctttg attttgacgg tgtaatactt aacagtgaac ccatgcattt tgaagcgtta
      241 gtccaggtac tgaatcaatc ggggattaat cttgcttatg aagaatatat gacccattac
      301 ctgggattaa gcgatattag tgtgtttccc aaaatactga atgataaggg tcttgcattc
      361 tcatccactg aaatacgtca acttatagag cgcaaggtca cagtctataa tgagttaatc
      421 gaggacagtg agcaattgcc tatgactctg gatctggatt ggtttttagt tcgagtggct
      481 aaacaatatg gaaaaatcgg tatctgcagc ggctccaatc gccattcaat aataaaaatt
      541 cttgaaaaaa ttcattgcgg tcgcttggct tgctatttta aaaccattgt aagttgtgaa
      601 gatgtaagct taggtaaacc gtctccagaa ggttatttgc tcgcagcaca tcgtttgcaa
      661 tctaatcctg aaaactgttt ggttatagag gattcagaac acggagtagc agctgcgaaa
      721 gcgggaggaa tgttggttgc gggtttactg acaacactca gcagggatca gctggccaat
      781 gcggatatga tcgtgcatga ttttaaagag ctggctcatt tgttggactc gcaataaaaa
      841 gagacaggtt atgcaggagg tctactataa agactatact tgatgttatt agaaaaataa
      901 tgaactacaa aattctgaag ataagaagag caagataatt tt
//
LOCUS       DASLOE010000338          942 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.340, whole genome shotgun sequence.
ACCESSION   DASLOE010000338 DASLOE010000000
VERSION     DASLOE010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.340"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..815)
                     /locus_tag="U6309_003030"
     CDS             complement(<1..815)
                     /locus_tag="U6309_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN5506427.1"
                     /translation="MSDTHCEKSYRSKLLKQGLLGVLILFLVFLFLESFSRWVAATST
                     APFGESIRNDQKMIVSDSIRQGIVAIGDSIIARSFYPELFSSLLAKNFSKPYEVYNIG
                     VEGSGIIHHRAHLRYLLKRNVKPDFVIMNVSVHNLNLNYFAHDSNEHIIEQRYEDHNS
                     LDYYNRCFTVNKQTLSSTLYCKIAKYSYFFRLVHYYHQQVNNSLQSLLHTQRLRTENL
                     IIKYPAISRQGFSPIFGSLAEDTISTPRMFEFLRHEYRSAFANFKLTTEVLDS"
BASE COUNT          338 a          164 c          141 g          299 t
ORIGIN      
        1 atgctgtcta aaacttcagt agttaactta aaatttgcaa atgcactcct atattcatgt
       61 ctcaaaaatt caaacattct aggcgtgctt attgtatcct cagcaagact gccaaaaatt
      121 ggtgaaaaac cttgtctaga gattgctgga tatttgatta tcaaattctc agttctcaat
      181 ctttgtgtat gcagcaaaga ttgcaaacta ttgttaactt gttgatggta ataatgcact
      241 aaacgaaaaa aataagaata cttcgctatc ttgcaataaa gcgttgatga aagtgtttgc
      301 ttatttactg taaaacaacg gttataataa tccaaggagt tatgatcttc atatctctgc
      361 tctatgatat gttcattact atcatgggca aaataattca aattaagatt atggacagaa
      421 acattcataa tcacaaaatc tggtttgaca ttccttttca ataaatatct taagtgtgcc
      481 cgatgatgga taattcctga accttccact cctatgttat acacctcata aggctttgaa
      541 aaatttttag ctaataaaga agaaaaaagt tccggataaa aacttctggc aattatggaa
      601 tccccaatag caacaatacc ttgcctgatg gagtcactca caatcatttt ctgatcattc
      661 ctgatacttt caccaaaagg tgctgtactt gtagctgcta cccatcttga gaaagattcg
      721 agaaacaaaa aaaccaggaa taaaatcaat acaccaagca aaccctgttt gagaagtttt
      781 gatctgtagc ttttttcgca atgtgtatcg gacataaata acagaaatta attttacaat
      841 attcgaattt gcgcagaata aacatatttg ataatgatgt atagtattaa aagctataaa
      901 aaaactgaag aaataattta gtttaaacgt aatccgctcc ct
//
LOCUS       DASLOE010000339          940 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.341, whole genome shotgun sequence.
ACCESSION   DASLOE010000339 DASLOE010000000
VERSION     DASLOE010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.341"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>940
                     /locus_tag="U6309_003031"
     CDS             <1..>940
                     /locus_tag="U6309_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5506428.1"
                     /translation="LNTVLLDTTTLYWNQLHEYSTLLNQCRITLQKSHRLTEEGQLAL
                     RCFNNAIQKMPSKKHIINALKILNKSNNKTEVIEKIGFVSPHGREACMQLTCWGKKFF
                     YIPRSEEMYHFDYDPKEDLNDTGGNCFGESMMFIHFLSKGAIRWLCPEAGLINFQLDQ
                     TRNLKLKKTALGEGETVVSDNSIHDSLQWKDMKPVLLDNPYFKPGSLCGVIFSMNDYT
                     KAQREFTAGHIIVVAKLDTNLNPYKYIVYEKDFGAFGLTDDESLEYLISEQILPLYSG
                     MNYSKIKLVKYGEASTETYDLLNEIKPQTSIPQKES"
BASE COUNT          327 a          144 c          181 g          288 t
ORIGIN      
        1 tacttaacac tgttttactg gataccacca ctctgtattg gaaccaatta cacgaatact
       61 caacattatt aaatcagtgt cgcattactt tgcaaaaatc tcatcgactt accgaagaag
      121 gacaattggc gcttcgctgt tttaataacg cgattcagaa aatgccatcc aagaaacata
      181 ttattaatgc gttaaaaata ttgaataagt caaataacaa gacagaagta attgaaaaaa
      241 taggctttgt ttcacctcat ggtagggaag cctgtatgca actaacttgt tgggggaaaa
      301 aattttttta catacctcgt agcgaggaaa tgtatcattt tgattatgat cctaaagagg
      361 atttgaatga tactggcggt aattgttttg gtgagtcaat gatgtttatc cattttttat
      421 ctaagggggc aattagatgg ttgtgtcccg aagcagggct aattaatttt cagttagatc
      481 aaacaagaaa tttaaaatta aaaaaaacag ctttaggtga aggggaaacg gtggtatccg
      541 ataatagtat tcatgattct ttacaatgga aggatatgaa gcctgtgctt ttagataatc
      601 cttactttaa accaggcagc ctgtgcggtg tcattttctc gatgaatgat tacacaaaag
      661 cacaaagaga gtttaccgca gggcacatta tagtagttgc aaagctggat actaacttga
      721 atccgtacaa gtacattgta tatgaaaaag attttggtgc atttggatta actgatgatg
      781 aatctttgga atacctaatt agtgaacaaa tattaccact ctatagtggg atgaattatt
      841 caaaaatcaa attggttaaa tatggtgagg cttcaactga gacctatgat cttttaaatg
      901 aaattaagcc tcagacaagt attccacaaa aagagtcaat
//
LOCUS       DASLOE010000340          934 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.342, whole genome shotgun sequence.
ACCESSION   DASLOE010000340 DASLOE010000000
VERSION     DASLOE010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.342"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..427
                     /locus_tag="U6309_003032"
     CDS             <1..427
                     /locus_tag="U6309_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506429.1"
                     /translation="CNSRSVPWVYQLSFVFYDYILDKEFEFITTKSGRDIFDAKKQLV
                     LKYIQKAADLYPRYEKEDFSDCHEMVNILLLSMRSEENNLQKRRNQDSSTSYMYSYFT
                     QTLYNASGKLMGKGQLGQKIDLLIEEFNERVVNKTPLYT"
     gene            complement(530..>934)
                     /locus_tag="U6309_003033"
     CDS             complement(530..>934)
                     /locus_tag="U6309_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin"
                     /protein_id="HEN5506430.1"
                     /translation="NWQNLCPVSGISGILNSIRMSFVEIRSTDTCIKTLIDKLNEIEI
                     GHFLIFVFKAALKNYDKHDFILLLKNLKHLHHSIELIRNKPFHSDDRFYGQWSFEGHD
                     PKRILEFIKSDDISGLMTILEDKKALPNNKPN"
BASE COUNT          316 a          146 c          166 g          306 t
ORIGIN      
        1 ttgcaatagt agaagcgtac cctgggtgta tcagctttcc tttgtattct atgactatat
       61 ccttgataag gaatttgagt ttataaccac taaaagcggc agagatattt ttgatgcaaa
      121 gaaacagttg gttcttaaat acatccaaaa ggcagctgat ctttatcctc gctatgaaaa
      181 agaagatttc tctgattgcc atgagatggt gaatatcctg ttactcagca tgcgttctga
      241 agaaaataat ttacaaaagc gcagaaatca ggatagtagt acaagttata tgtactctta
      301 ttttacacaa acactttata atgcttccgg gaaattaatg gggaaggggc aattagggca
      361 aaaaatagat ttattgatag aagaatttaa tgaaagagta gtcaataaaa cacccttgta
      421 tacttaattc tttattttcc aaagcaaaac agaaaggtca ctggattaat agtaaaaaca
      481 ccaattcaag ttgatttgtt aactcagtga cgcgttggat ttaaacctgt taattcggtt
      541 tattattggg aagcgccttt ttatcttcta aaatagtcat taatcctgaa atatcatcgc
      601 ttttaatgaa ttcgaggatt cttttgggat catgcccttc aaaactccac tgtccataaa
      661 atcggtcgtc agaatgaaac ggcttatttc tgattagctc gatagaatga tgaagatgct
      721 tcaggttttt caatagtaat atgaagtcat gtttatcgta attttttaaa gccgctttaa
      781 aaacaaatat caaaaaatgt ccaatttcaa tttcgttcag tttatcaatt aatgttttta
      841 tacaggtatc tgttgaacgg atttcaacaa atgacatgcg gatagaattt aatataccac
      901 taatgccgga aacggggcat aaattttgcc agtt
//
LOCUS       DASLOE010000341          931 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.343, whole genome shotgun sequence.
ACCESSION   DASLOE010000341 DASLOE010000000
VERSION     DASLOE010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.343"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            93..931
                     /locus_tag="U6309_003034"
                     /pseudo
     CDS             93..931
                     /locus_tag="U6309_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947220.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          371 a          136 c          181 g          243 t
ORIGIN      
        1 ggatagaaga cgtaatttcc gcaagaaaaa aagcggcttt atctcccagc actccggtgt
       61 ctatggataa aaaagcacct gatccagcca tggaattagc aaagcttaaa gaaaaatgtc
      121 tgaagatgga agaagatgag tttaattatt gttttaacaa aggaaatact tcagaacgca
      181 gcattagtac aatcattgcg cggctcaaga acgcagcagg tcttaaagaa gagtttttca
      241 agacagacaa agaatatatt gactggatag aagacgtaat ttctacaaga aaaaaagcgg
      301 ctttatctcc cagcaccccg gcgtctatgg ataaaaaagc acctgatcca gccatggaat
      361 tagcaaagct taaagaaaaa tgtctgaaga tggaagaaga tgagtttaat tattgtttta
      421 acaaaggaaa tacttcagaa cgcagcatta gtacaatcat tgcgcggctc aagaatgcag
      481 tagatcttaa ggaagagttt ttcaagacag acaaagatta tgttgtctgg atagaaaacg
      541 taatttctgc aagaaaaaat gttcccatag agttagcaaa gataaaagaa caattattga
      601 aaatgaaaga aggtgagttt gattattatg ttaaagattt aagtaccaat gaatacaatg
      661 gcggcatatc tattaaccga cgactttttc gagatttagg tcttaagggg gagtttttca
      721 agacacaaaa agattataac gcatggatag aagacgtaat ttctgcaaga aaatttaata
      781 attttagtac caatattgat gatattttaa ataaatttga agaaaaaata aaaaatatag
      841 atactaatta tccagaggca aaaatcaaag ccaatgagtt gttgatatcg cttagaaaaa
      901 ataaagacga agcattttca aatccttccc t
//
LOCUS       DASLOE010000342          917 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.344, whole genome shotgun sequence.
ACCESSION   DASLOE010000342 DASLOE010000000
VERSION     DASLOE010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.344"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..614
                     /locus_tag="U6309_003035"
     CDS             <1..614
                     /locus_tag="U6309_003035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506431.1"
                     /translation="TNVKKLCGLLAMYSNHEVWKQSLSEHEQEIFEKKTPYKRLDLDP
                     HNMKELLSILDGIASNAFAPEGQLLYLSNSQGRYHFDRDELLIKNFVKDVDNEVNYDF
                     KEQLEKQTFVINTPKINTSPTSKCSNISMQILGGFMAVTGVATVALAFVILNAATFGL
                     AGLIAAGVGTAGILGGVGLFAVGASRSCPSTIDEPLNNPFTPV"
BASE COUNT          287 a          155 c          175 g          300 t
ORIGIN      
        1 aaacaaatgt taaaaaacta tgcggcttac ttgctatgta ttctaatcat gaggtttgga
       61 aacagagttt atcagaacac gaacaagaaa tttttgaaaa aaaaactcca tataaaaggc
      121 ttgatctaga tcctcacaat atgaaggaat tgctctctat tttggatggt attgcaagca
      181 atgctttcgc accagaaggt caattgcttt atctatcgaa ttcacaagga agatatcatt
      241 ttgatcgtga tgagctatta atcaaaaatt tcgttaaaga cgttgataat gaagtaaatt
      301 atgactttaa agaacagttg gaaaaacaaa cttttgtaat caatacgcct aaaattaata
      361 cttccccaac atctaaatgt tcaaatatta gcatgcagat tttaggtggg tttatggctg
      421 tcactggtgt cgccacagtt gccctcgctt ttgtgatact taatgctgca acttttggtc
      481 tggctggttt aatcgctgca ggcgtgggaa ctgctggcat tcttggaggt gtaggattat
      541 ttgctgtagg ggcctctaga agttgtccat ctactataga tgagccatta aacaatcctt
      601 ttactcctgt ttaaaattag aaaaataaaa gaatagggct cttgcatagc cctggaattg
      661 attaatcttt ttgtcttttt gcaagaatgg gttggggaac gatggtcagt tcggttagaa
      721 gttcccttcc tggaacccca agttgaccct aattattaag tccacaggct gctattatcg
      781 gatttttatg cttataatga cctagatagt gttgtctata tgctgccatt tctaaccgta
      841 gagtttattt atgatggtaa tacaacagct tttataattt gacgacaata tccaagcaat
      901 ttaccaaaaa attcaaa
//
LOCUS       DASLOE010000343          913 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.345, whole genome shotgun sequence.
ACCESSION   DASLOE010000343 DASLOE010000000
VERSION     DASLOE010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.345"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..899)
                     /locus_tag="U6309_003036"
     CDS             complement(<1..899)
                     /locus_tag="U6309_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010654170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN5506432.1"
                     /translation="MDIPNERNLHSRPTVRGGGVVFIGLSLFCWFFICNSQFFLTLDQ
                     FIFSIAIILIATISFIDDFYNLSVTIRFFFQCLIAFLIVIFILPSQLDFGLFLFENTY
                     LIAFFLFFAVIWAINHFNFMDGLDGFCASQAIFLLAAYALFLNSAGSLIYQDFCLILI
                     SSLAGFLIFNFPPAKIFMGDVGSASLGLITFFIAIIAQKNYQIPILYWFILNSLFLFD
                     STVTLIRRIIKHEKWFSPHKKHAYQRLKQLGINSRFILLGQMLLNCSFLSIILLHRII
                     GFNLNYLMLLQLICLLFLYIIVE"
BASE COUNT          389 a          137 c          131 g          256 t
ORIGIN      
        1 ttttctacta ttatgtatag gaataataaa caaattagct ggagcaacat gagataattg
       61 agattaaaac caattatccg atgcaataga ataattgata aaaaactgca atttaataac
      121 atttgaccta ataaaataaa cctcgagtta atgcccaatt gctttaatct ctgataagcg
      181 tgctttttat ggggactaaa ccatttttca tgctttataa tccttcgaat taaagtcact
      241 gtagaatcga aaagaaataa tgaattcagg ataaaccaat atagaattgg aatttgatag
      301 ttcttttgag caatgattgc tatgaaaaaa gtaattaatc ctaaggaggc gctacctaca
      361 tctcccataa atatcttggc tggtgggaaa ttaaaaatta aaaaccctgc taaactagaa
      421 ataagaatca ggcaaaaatc ttgataaatt aatgaacctg ctgagtttaa aaacaacgca
      481 tatgcagcta acaaaaaaat cgcttgggaa gcgcaaaaac catcaagccc atccatgaaa
      541 ttaaaatgat taattgccca aataactgca aaaaatagaa aaaaagcaat taaataggtg
      601 ttttcaaata gaaataaacc aaaatctaat tgggaaggca atataaatat aactatcagg
      661 aatgcaatca gacattgaaa aaagaatcga attgtaactg acaaattata aaaatcatct
      721 ataaaactta tagttgctat taaaataata gctatcgaaa aaatgaattg atccaatgtt
      781 aaaaaaaatt gtgagttaca aataaaaaac caacaaaata aactaagtcc aataaagacg
      841 acccctcctc ctcggactgt aggtcttgag tgtaaatttc tttcatttgg aatatcaatc
      901 aattttgtat gct
//
LOCUS       DASLOE010000344          910 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.346, whole genome shotgun sequence.
ACCESSION   DASLOE010000344 DASLOE010000000
VERSION     DASLOE010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.346"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            99..174
                     /locus_tag="U6309_003037"
     tRNA            99..174
                     /locus_tag="U6309_003037"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:132..134,aa:Thr,seq:tgt)
     gene            247..331
                     /locus_tag="U6309_003038"
     tRNA            247..331
                     /locus_tag="U6309_003038"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:281..283,aa:Tyr,seq:gta)
     gene            352..425
                     /locus_tag="U6309_003039"
     tRNA            352..425
                     /locus_tag="U6309_003039"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:384..386,aa:Gly,seq:tcc)
     gene            478..553
                     /locus_tag="U6309_003040"
     tRNA            478..553
                     /locus_tag="U6309_003040"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:511..513,aa:Thr,seq:ggt)
     gene            588..>910
                     /gene="tuf"
                     /locus_tag="U6309_003041"
     CDS             588..>910
                     /gene="tuf"
                     /locus_tag="U6309_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN5506433.1"
                     /translation="MAKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTA
                     KAYDQIDAAPEERERGITISTAHVEYESASRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSA"
BASE COUNT          274 a          177 c          235 g          224 t
ORIGIN      
        1 tatagtgtgg catgtaaagc caactaaatt aaaataggtt agttgtatac gcataatata
       61 aattaaataa gcgctaacct atcatatgta aagaatatgc tggcgtagct cagttggtag
      121 agcaactgac ttgtaatcag taggtcccgg gttcgattcc tggtgccagc accatctaaa
      181 tagggctaaa cgttgactaa aaatgttgac aatccattca tcttaaaaga caatagcgtt
      241 ctttttggag gggttcccga gcggtcaaag ggatcagact gtaaatctga cggctcagcc
      301 ttcgaaggtt cgaatccttc cccctccacc agttaagaga gaagttaaaa agcgggtgta
      361 gttcaatggt agaacttcag ccttccaagc tgatagcgtg ggttcgattc ccatcacccg
      421 ctccaaagta gtgaatagta tttgcttgtt ggtgtgagtt agcaagctga tatgaaagcc
      481 cacatagctc aggcggtaga gcacttcctt ggtaaggaag aggtcgttgg ttcgagtcca
      541 gttgtgggca ccatgtaata taaattagca aacggggaga ctttccgatg gcgaaggaaa
      601 aatttgaacg taagaagccg cacgtaaacg tgggcacgat tggtcacgta gaccatggca
      661 agacgacatt gacagcagct attacaacga ttatggcgaa gaaatatggt ggtacagcga
      721 aggcgtacga tcaaattgat gctgcgccag aagaaagaga gcgtgggatt actatttcta
      781 cagcacatgt tgaatacgaa tcagcgagca gacattatgc gcacgtagac tgcccggggc
      841 atgctgacta tgttaagaac atgattactg gtgctgcgca aatggacgga gcgatactgg
      901 ttgtatcagc
//
LOCUS       DASLOE010000345          894 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.347, whole genome shotgun sequence.
ACCESSION   DASLOE010000345 DASLOE010000000
VERSION     DASLOE010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.347"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..858)
                     /locus_tag="U6309_003042"
     CDS             complement(<1..858)
                     /locus_tag="U6309_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEN5506434.1"
                     /translation="MNFVTFQFVVFLVITVIVFMMMSPYYRKYFILLCSYFFYGTWSV
                     PLISVILFSTSVDYYLGKKIHESHSQTHKKLYLTLSILVNCSILVYFKYMNFFISSID
                     AIGFSLGATQLLLNPMHIILPLGVSFYTFEAISYIVDIYRGEKPASSWLNYNFYIMYF
                     PHLISGPIVRYKELHAQYESKIELPETSRLKEGFQLLLYGFIFKLIIADSMASISDPL
                     FDKYQTIGHLESWLASIAFTIQIYFDFLGYTHIARGVSLFFNIKLPVNFNHPYNARNI
                     SEFWQRWHIT"
BASE COUNT          353 a          149 c          151 g          241 t
ORIGIN      
        1 agtgatatgc caacgctgcc agaattcact gatattgcgc gcattataag gatgattaaa
       61 attaacaggt aatttaatat taaaaaataa tgatacaccc cgcgcaatat gggtatatcc
      121 aaggaaatca aaataaatct gtatggtaaa tgcaatactg gccaaccagg attccagatg
      181 accaattgtc tgatacttat caaacaaggg atctgagatt gaggccattg aatcggctat
      241 gatcaatttg aaaataaagc cataaagtaa taactgaaac ccctctttta aacgcgaagt
      301 ttccggtaat tcaattttac tttcatattg agcatgcagt tctttataac ggacaatcgg
      361 tcctgaaatc agatgcggga aatacataat ataaaaatta tagtttaacc agctactagc
      421 cggcttttcc cctctataga tgtctacgat ataactgatc gcctcaaaag tgtaaaaaga
      481 aacaccgaga ggcaaaataa tatgcatagg atttaacaaa agttgagtag ctcccaaact
      541 aaaaccaata gcatcaatac tggaaataaa aaaattcatg tatttgaaat aaaccagaat
      601 agagcaatta accaaaatgg ataaagtcaa atagagtttt ttatgagttt gggaatgact
      661 ctcatggatt ttttttccga gatagtaatc aaccgaggtt gaaaataaga tgacactgat
      721 taatggaaca ctccaggtac cgtaaaaaaa atagctgcaa agcaaaataa aatatttccg
      781 ataataggga gacatcatca taaaaacaat gaccgtaata actagaaaaa caacgaactg
      841 aaaagtgaca aaattcatgc tttgttctca ttattgcttt ttaggtattg aaac
//
LOCUS       DASLOE010000346          876 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.348, whole genome shotgun sequence.
ACCESSION   DASLOE010000346 DASLOE010000000
VERSION     DASLOE010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.348"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            161..541
                     /locus_tag="U6309_003043"
     CDS             161..541
                     /locus_tag="U6309_003043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506435.1"
                     /translation="MQVESILPEILKGTIHKARINSLTSLVKGIVCHKKLKLSELGRH
                     LHLTSERSGIRIVDRVLGNTYYQERSIELYSCIAKTVIGDNQTPDILIDWSVIPNSQR
                     CTNEKEEYQVLSVVAEYKLIPFAD"
     gene            596..>876
                     /locus_tag="U6309_003044"
     CDS             596..>876
                     /locus_tag="U6309_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN5506436.1"
                     /translation="MIQLEESKMIHILYKQGYSKKAIARKLGMSINTVRKYLKSGSEP
                     YYKRRDKKPLKLDPYKAYIQKRLKDATPHWVPATVIYREIKLFGYDGCSS"
BASE COUNT          309 a          141 c          182 g          244 t
ORIGIN      
        1 cacggtcgcg gctcaggttt aattatcatt tgatttaatc atccgagccg cgaccgtgag
       61 ggctgaggta tcgactcatt tttaagataa aagaaagaag acaagcatag caaaaccatg
      121 atctaatagt ttcgcgaaaa agaactagat aggttgagct atgcaagtgg aatccatttt
      181 acctgaaata cttaaaggta caattcataa agcaagaata aactctttaa ctagcctggt
      241 taaaggcata gtttgtcata aaaaattaaa attaagcgag ctaggtcggc atttacacct
      301 cacaagcgaa cgttcaggaa taaggattgt agatagggta ttagggaata cgtattatca
      361 agagcgctca attgagctct atagttgtat agctaagact gttattggtg acaatcaaac
      421 acctgacatt ttaattgatt ggtcagtgat accaaatagc cagcgctgta ctaatgaaaa
      481 agaagaatat caggttctca gtgtcgttgc tgaatataaa ctgatcccct ttgccgactg
      541 aaaattgatc cccctactat agttttcttc aggagaatga attctggagg agggaatgat
      601 tcaattagag gagtcaaaaa tgattcatat tttgtacaag cagggataca gtaaaaaagc
      661 gattgctaga aaattgggta tgtcgataaa tactgttcga aaatatctta aaagtggttc
      721 agagccttat tacaaaaggc gtgataaaaa gccattaaag cttgacccct ataaagcata
      781 tattcaaaaa agattaaaag atgcaacacc tcattgggta ccagctactg tgatttatcg
      841 agagattaaa ttgtttggtt atgatggatg cagctc
//
LOCUS       DASLOE010000347          871 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.349, whole genome shotgun sequence.
ACCESSION   DASLOE010000347 DASLOE010000000
VERSION     DASLOE010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.349"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..577)
                     /locus_tag="U6309_003045"
     CDS             complement(<1..577)
                     /locus_tag="U6309_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEN5506437.1"
                     /translation="MLLHYLFIVGICVEAITGALAAGRKKMDFFGVLLIASITALGGG
                     TVRDTLFNTYPLTWVAHPEYLLYTSVCAFLTIFIAHSLVRIMKVFLILDALGLSTFVI
                     IGTQKVLAHGMPPSVAIISGMITGICGGMLRDILCNDIPLVLRKELYAVIALAGALLF
                     ITLDYFHIPHNLNIIITLLCIFTARLLAIFFH"
     gene            694..>871
                     /locus_tag="U6309_003046"
     CDS             694..>871
                     /locus_tag="U6309_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506438.1"
                     /translation="MNNTIDNQSQEELTHFDANTPQSIIQLDRKLREMKSEVDMKLND
                     PSSYNGDDKEVYFQK"
BASE COUNT          330 a          153 c          158 g          230 t
ORIGIN      
        1 tatgaaaaaa aatagccaat aagcgggcgg taaaaatgca taacaaagtg ataatgatat
       61 ttaaattatg agggatatga aaatagtcta atgttataaa cagaagcgca ccagccaggg
      121 caataacagc atataattct tttcttaaaa ccagaggaat gtcattacat aaaatatctc
      181 gcaacattcc cccgcaaatc ccggtaatca ttccactgat tattgcaaca cttggtggca
      241 taccatgggc gaggactttt tgtgtaccaa taataacaaa agttgagaga ccaagggcat
      301 caagaattaa gaatactttc attattctaa ccagtgaatg agcaataaaa atagttagaa
      361 aagcgcatac tgaggtatat aaaagatatt ccgggtgcgc tacccaggtc agtggataag
      421 tgttaaataa tgtatccctt acggtccctc ctcccagtgc agtgattgac gcaataagta
      481 gcaccccaaa aaaatccatt tttttacgtc ctgctgccag ggctcctgta atcgcctcta
      541 cacaaatccc cacaataaaa agataatgaa gtaacatatg atttgctgct atttaaaaag
      601 acaatgatag cactaatagt acaaatgaag aaatgctgta cttcgactag aatttaatag
      661 taaggtttta attattttgg caggagcaaa ttgatgaata atacgatcga taatcaatcg
      721 caagaagaat taactcattt tgatgcaaat acgccccaga gcatcattca attggataga
      781 aagctcaggg aaatgaaaag cgaagtcgat atgaaactaa atgatccttc atcttataat
      841 ggggatgata aagaagttta ttttcaaaaa t
//
LOCUS       DASLOE010000348          847 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.350, whole genome shotgun sequence.
ACCESSION   DASLOE010000348 DASLOE010000000
VERSION     DASLOE010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.350"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(490..>847)
                     /locus_tag="U6309_003047"
     CDS             complement(490..>847)
                     /locus_tag="U6309_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506439.1"
                     /translation="LKELSLKNLDIDSCRMGRVESYRHELKHHLKNFQTVTTYTDLCT
                     ASQRGGVPYRMWIEERADRDVFYTESDLNKKGTRIIEYTDTYKNSLKEIWKTNPYNLE
                     EIDLSDHIDILVIASC"
BASE COUNT          262 a          145 c          130 g          310 t
ORIGIN      
        1 atcggttgtg atttggtaaa ataagatatc cacagacaac ggataaggga aaacattttt
       61 gacctgcata tcttatacaa ataaaaaata aaaaaacagt cttgactcga actattttta
      121 ctatttattt tgacccactg cttgattaaa ctttttgctt aaacaataac tctcctgcct
      181 tagtgaggtt tttaaatttg gaactatagc catcttgttt ggaacaacta aaatagcgag
      241 caagcctatg cctgataaat aggcaaacta atattatgtg gagatggttt gttcatgtgt
      301 tttaaaattg gcaacgtatc cattcttctt tggatttcaa atctctaata attttggtat
      361 ggttgcttaa tgttggtgtc cggcttttct aggaaggatc agtctgagat gttatgactt
      421 aacgcatcac acagtttaaa gattgaaaaa tttgcgagtt acccaactca attcaagcta
      481 ttgtcgaaat tagcatgatg caattactaa aatatcaata tgatcagaca gatcaatttc
      541 ttccagatta tacgggtttg ttttccaaat ttcttttaat gaatttttat aggtatccgt
      601 atattcaata atgcgggtcc cctttttgtt tagatcactt tcagtataaa aaacatctct
      661 atccgctctt tcttctatcc acattctgta aggcactcca cccctttgtg atgctgtgca
      721 taaatcagta tatgttgtaa cagtttgaaa attctttaaa tggtgtttta attcatgacg
      781 ataactttca actcttccca tcctgcaaga atctatatct aaatttttta gtgataattc
      841 ttttaac
//
LOCUS       DASLOE010000349          803 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.351, whole genome shotgun sequence.
ACCESSION   DASLOE010000349 DASLOE010000000
VERSION     DASLOE010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.351"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(121..>803)
                     /locus_tag="U6309_003048"
     CDS             complement(121..>803)
                     /locus_tag="U6309_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506440.1"
                     /translation="VRDRGIHCYGSWSLKSYSINDEGQVHAYIYCLGQLPHKEQLHWK
                     QYNEEPKAGISKVAYNTDFLAEFDEEESSLRAIKQKLANFPPYSLDKEKFVLWAPKGG
                     DIDMLLKQIHPVITDIKKEYLDYLLNLTILVIDGLQEKVIRRLVNAEENKRSLECLEI
                     LLKNWGSPNADKIMKALRCLQAKRSKYAGHGGGKIDFDIKEDSLAITYEINEAIHLLS
                     EEIDKNSR"
BASE COUNT          258 a          175 c          104 g          266 t
ORIGIN      
        1 ataaaatcaa gtagttacga gtagtaaaaa ctgaaacggt ctgaggggca ctgatggggc
       61 actcagaaat aacaaatagc aactcatccc aataacatgg ggcatttaaa ataaattcct
      121 ttacctgcta tttttatcaa tttcctcact taacaagtgt atagcctcat taatttcata
      181 agtaattgcc aaagaatcct ctttaatatc aaaatcaatt ttaccaccac catggccagc
      241 atatttactt cttttagctt gcaagcacct tagtgctttc attatcttat ctgcatttgg
      301 agaaccccag tttttcaaaa gtatctccaa acactcaagg ctcctcttat tctcttcagc
      361 atttactaat ctacgaatga ccttttcctg taatccatca ataactaaaa tagtcaaatt
      421 caacaaataa tctaaatact cttttttgat atcagtaata actgggtgaa tctgctttaa
      481 tagcatatca atatcacccc ctttaggagc ccataataca aatttttcct tatctaaaga
      541 ataaggagga aaattggcta atttttgttt tatcgctcta agagaacttt cttcttcatc
      601 gaattcagct aaaaaatccg tgttataagc gacttttgaa atccccgctt ttggctcttc
      661 attatactgc ttccaatgaa gctgttcttt atggggtaat tgccctaaac aataaatata
      721 agcgtgcacc tgtccttcat catttattga gtaagacttc aaactccaac ttccatagca
      781 atgaattccc cgatctctga cct
//
LOCUS       DASLOE010000350          758 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.352, whole genome shotgun sequence.
ACCESSION   DASLOE010000350 DASLOE010000000
VERSION     DASLOE010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.352"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            46..>758
                     /locus_tag="U6309_003049"
     CDS             46..>758
                     /locus_tag="U6309_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN5506441.1"
                     /translation="MKIIHISDLHFGKHQPAVLNAFLQETALDKPDIILISGDLTQRG
                     LSYQYRELCSFINQLPAQTLTVPGNHDIPFYNVPARFIFPFRHYKRYINPDVTTTFEN
                     DCVRILGVNSVNPLQLKDGELSRETLNRIKRYFKPDDEKLNLLFFHHNFDYMEGLHKP
                     LANAQTLLSFLKQSTIHLVCTGHLHYAHLSLIAKNNGYSCLALHAGSLLCPRSKDNLN
                     SYYVIEANQKKCRITWRVF"
BASE COUNT          237 a          158 c          123 g          240 t
ORIGIN      
        1 ttataaaatt ttacctaaat cactaaaatt actgactaaa aaaatatgaa aattatccat
       61 atttccgatt tgcattttgg caagcatcaa cctgcagttt taaatgcgtt tcttcaggaa
      121 accgccctcg ataaaccaga catcattctt atttcaggag atttaactca acgtggactc
      181 tcgtatcagt accgggagct ttgctcattt ataaatcaac tgccagctca aacccttact
      241 gttcccggta atcatgacat tcccttttat aatgtcccag cacgttttat attccctttt
      301 cgccattaca aacgctacat aaacccggat gtaaccacca cctttgaaaa cgattgtgtg
      361 cgtattttgg gggttaatag tgtcaacccc ttgcaattaa aggatggaga actatctcgt
      421 gaaacattga atagaattaa acgatatttt aaaccggatg atgaaaaatt aaacttgctc
      481 ttctttcatc ataattttga ttatatggaa ggattacata agccactagc aaatgcacaa
      541 acgcttttaa gttttttaaa gcaaagtacc attcatttgg tttgcacggg tcatctacac
      601 tatgctcatt taagtttaat tgctaaaaat aatggctact cctgcctggc gctgcatgcc
      661 ggctcattac tgtgtccacg cagcaaagat aatttaaaca gctattatgt gattgaagcg
      721 aatcagaaaa agtgccgcat tacctggcgt gtttttga
//
LOCUS       DASLOE010000351          727 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.353, whole genome shotgun sequence.
ACCESSION   DASLOE010000351 DASLOE010000000
VERSION     DASLOE010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.353"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..538)
                     /locus_tag="U6309_003050"
     CDS             complement(<1..538)
                     /locus_tag="U6309_003050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506442.1"
                     /translation="MMKQLYSPVFIDVFATYARDRFTLVNTVSGGDLPTPLTGFSKYY
                     GKDNTIGARTFFGYPYKKLFLQGYLTYFYSQFYQPNYIVSYPTQNVIVPELVTKIGTT
                     IEHARLYYQVNDHVSPFVSGGLIQLLSRSFSRPVIDPAVTAVSALPQILLDKSGYSVG
                     AGVDFNFRALRLTPVYVYS"
BASE COUNT          270 a          136 c          120 g          201 t
ORIGIN      
        1 cagaataaac ataaacaggg gttagccgca aggctctgaa attaaaatcc actccagccc
       61 caacactgta cccactcttg tctaaaagaa tctggggaag agcagataca gctgtcacag
      121 caggatcaat aacaggtcga ctaaatgagc gacttagcaa ctgaattagc ccaccactta
      181 caaaaggaga gacatgatca tttacttgat agtacaatct ggcatgttca attgtagttc
      241 caattttagt aactaattct ggcacaataa cattttgcgt tggataagaa acaatataat
      301 ttggttgata aaattgacta taaaaataag ttaaataacc ttgcagaaac aacttcttat
      361 agggataacc aaaaaatgta cgcgcaccta ttgtattatc cttaccataa tatttggaaa
      421 atcctgtaag gggggttgga agatcacctc cactcactgt gttaacaagc gtaaatctgt
      481 cccgcgcata tgttgcgaaa acatctatga aaacaggaga ataaagttgt ttcatcacat
      541 gaaaataaaa cccgttatcc tcaattatat tctgaccctg atttagattg ttagaaagga
      601 gtgatgacac actcatatta ctagttaaat tatatccatt aaatccccag tataaattgt
      661 aaaatcttaa ataatcacca ccggcagaat tataatttgt atgtccagaa tagttattaa
      721 agaaaat
//
LOCUS       DASLOE010000352          710 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.354, whole genome shotgun sequence.
ACCESSION   DASLOE010000352 DASLOE010000000
VERSION     DASLOE010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.354"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>710)
                     /locus_tag="U6309_003051"
     CDS             complement(<1..>710)
                     /locus_tag="U6309_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014842397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEN5506443.1"
                     /translation="AVALPEVYTNKNLIINKLLLNIYEQDLLFLANGNACEAKKDFAN
                     FYNPYYTAIGKALLPKLEQLAFNFLKDEIEIIGNWTKPKLLDYLINTILEQEKETSEL
                     CEAIVGSNNPIRAAKMLLIQMAPDFLSEASAMARALPGSFGIEQSELMKVFIDEYGYG
                     VHETKHSTLFEATMLSANLNPTIHYYYNDYLATSLMLVNYFHCICKNKSDWFKYIGTL
                     YYTEASIPHFNKQISKTL"
BASE COUNT          225 a          120 c          118 g          247 t
ORIGIN      
        1 ttaaggtttt actaatttgt ttgttaaaat gagggatgct tgcttcagtg taatataacg
       61 taccaatata tttgaaccaa tcagatttat ttttgcatat acaatgaaag taattaacga
      121 gcattagtga cgtggctaga taatcattat agtaataatg tatagtggga tttaaattgg
      181 ctgaaagcat cgtagcttca aacaaagttg agtgttttgt ttcatgaacc ccataaccat
      241 actcatctat aaataccttc attaattctg attgttcaat accaaaggag cctggtaacg
      301 ccctcgccat tgccgaagct tcactaagaa aatcaggcgc catttgaatg agtaacatct
      361 tggctgcccg tataggatta ttagaaccaa ctatagcctc gcaaagctca ctagtttctt
      421 tttcttgctc cagaattgta tttatcagat aatctaataa ttttggtttg gtccaattac
      481 ctattatttc aatttcatcc tttaaaaaat tgaatgctag ctgttcaagt tttggcaaca
      541 atgctttgcc aatagctgta taatatggat tatagaaatt agcgaagtct ttcttggctt
      601 cacatgcatt gccattggcc agaaacaaaa gatcttgttc ataaatatta agcaagagct
      661 tatttatgat tagattttta ttagtatata cttcaggtaa agccactgct
//
LOCUS       DASLOE010000353          691 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.355, whole genome shotgun sequence.
ACCESSION   DASLOE010000353 DASLOE010000000
VERSION     DASLOE010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.355"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>691
                     /locus_tag="U6309_003052"
     CDS             <1..>691
                     /locus_tag="U6309_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506444.1"
                     /translation="RVLEHKTNVVETLVRHLKDENFKCLKVDSNQLKAKYPCLVVTFK
                     HDEYRNKPENIPEGYSEFVTHMMIAQINLKLKEKNLSPLIDRRQSFGFLTPTLTDVHT
                     GVRLSLGLTPNKQWIECVEEGIKQTDKSLAKFTLKDKDDLLNLFRAGEFCGDGHKYLY
                     DLYKTGEKTQEEVKKFLQESKVKSECKDIYFSTKDITCIDKDKLKKGVLKRFYYFDSK
                     NGAITLYDNQHQ"
BASE COUNT          274 a           96 c          127 g          194 t
ORIGIN      
        1 tagggtattg gaacataaaa caaacgtggt agaaaccctg gttcggcatt taaaagacga
       61 aaattttaag tgtctaaagg ttgatagcaa tcaacttaaa gccaaatatc cttgtttagt
      121 tgttaccttt aaacatgatg agtataggaa taaacccgaa aatatccctg aaggatactc
      181 tgagtttgtt acccatatga tgattgctca gattaattta aaattaaaag aaaaaaatct
      241 gtccccattg atagataggc gacaaagttt tgggtttctc acccctacat taactgatgt
      301 tcatacgggt gttagattat cacttggcct tacaccaaac aaacagtgga tagaatgtgt
      361 tgaagaagga ataaaacaga cagataagtc cttagcaaaa tttacgttga aagacaaaga
      421 tgatttactc aatctattta gagcgggaga gttttgtggg gatggacata aataccttta
      481 tgacttatat aaaacaggag aaaaaacaca agaagaagta aagaagtttc tacaagaatc
      541 aaaagtgaaa tcagaatgta aggacattta tttttcaaca aaagacataa cgtgtattga
      601 taaagataaa ttaaaaaaag gcgtattgaa aagattttat tactttgata gtaaaaatgg
      661 agcaattact ctttatgata accagcacca a
//
LOCUS       DASLOE010000354          672 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.356, whole genome shotgun sequence.
ACCESSION   DASLOE010000354 DASLOE010000000
VERSION     DASLOE010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.356"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..311)
                     /locus_tag="U6309_003053"
     CDS             complement(<1..311)
                     /locus_tag="U6309_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEN5506445.1"
                     /translation="MYNKLTTSQATYLNFLRGFSAIIVLAGHTLSGIPGVISFGKQLP
                     FQSLAVNAFFWLSGFLITYHCITKQPYSFVEYMIDRFCRIYVIYIPVLILSVLLLVWV
                     D"
BASE COUNT          264 a          100 c           96 g          212 t
ORIGIN      
        1 aaatccaccc atactaacaa caaaacagat aaaatcaata ctggaatata aataacataa
       61 attctgcaga accgatcaat catgtattct acaaatgaat agggttgctt tgttatgcaa
      121 tgatatgtaa tcaaaaatcc tgagagccaa aaaaaagcat tcactgccaa ggactgaaaa
      181 ggcagttgtt tcccaaatga aattacccca gggatacctg ataaagtatg tccggccaag
      241 actattatag ctgaaaaccc tcggagaaaa tttaggtagg ttgcttggct tgttgtgagt
      301 ttattataca tttaaaaaac cattccttta gatagtatca acaataaatt aaattgtttt
      361 caaattatat ataaaaaaaa taagtaaaga aattaaatta gttaaattat tcttgcatgt
      421 aatttaggta atatcacttt gattcttgca agccttgcaa agtatctatt atattttgag
      481 ttgattcaat catttgctga taactatttt tattaaaaaa tttttttaca tcatttaaag
      541 ttaaggcatg aacaattttt tttctgccgt ataaataaga caaaaaaata gaggatgagg
      601 agacagaaac aatattacat ttataaatca gtccagttag catttccaac ggaatacatc
      661 gcaaactttc ag
//
LOCUS       DASLOE010000355          660 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.357, whole genome shotgun sequence.
ACCESSION   DASLOE010000355 DASLOE010000000
VERSION     DASLOE010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.357"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            58..>660
                     /locus_tag="U6309_003054"
     CDS             58..>660
                     /locus_tag="U6309_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEN5506446.1"
                     /translation="MKSHTELLSWNLLQKEADRVRLNSDSLTCVVPDSNNYESSKQIN
                     CIEYDYSRQRVNRTIIDLLIDLANEVKLQEKIDNLINGKKINISENRPALHTALRDLG
                     NKSIMIDGLDIMSAVINTREKIKVISNQIREKKWLGHSGLPITDIVNIGIGGSDLGPR
                     VCINALSNYISKEFNYHFISDVDPASFNDVIAKINPQTTLF"
BASE COUNT          236 a          107 c          117 g          200 t
ORIGIN      
        1 ttaataaatt gaaaataatc aggtaacata gactttattt tgatgattag aaatagcatg
       61 aagtctcaca cagaattatt gtcatggaat ttgcttcaaa aagaagcgga tagagtgcgt
      121 ctaaattcag attctctaac atgcgttgtt ccggattcca acaattatga atccagtaaa
      181 cagatcaatt gtattgagta cgactattcc aggcagagag taaacagaac catcattgat
      241 ttgcttatcg atttggctaa tgaagtcaaa ttacaagaaa aaattgataa cttaatcaat
      301 ggtaaaaaaa tcaatatcag cgaaaacagg cctgcactac atactgcact tagggactta
      361 ggtaataaat cgataatgat tgatggactg gatattatgt ccgcagttat aaatactcgt
      421 gaaaaaatta aagttatatc caatcagatc agagaaaaga agtggctggg ccattcaggt
      481 ctaccaataa ctgatattgt aaacataggt attggcggct cggatttagg tcctcgtgta
      541 tgcatcaatg ccctgtctaa ttatatttcc aaagaattta attatcattt tatctcagat
      601 gttgatccgg ccagttttaa cgatgttatt gcaaaaatta atccgcaaac caccttattt
//
LOCUS       DASLOE010000356          659 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.358, whole genome shotgun sequence.
ACCESSION   DASLOE010000356 DASLOE010000000
VERSION     DASLOE010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.358"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(16..92)
                     /locus_tag="U6309_003055"
     tRNA            complement(16..92)
                     /locus_tag="U6309_003055"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Arg,seq:cct)
     gene            545..>659
                     /locus_tag="U6309_003056"
     CDS             545..>659
                     /locus_tag="U6309_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5506447.1"
                     /translation="MKIFKGLKKNTGFTKQSNTQKISNSSIQLSITSLSGDA"
BASE COUNT          214 a          108 c          132 g          205 t
ORIGIN      
        1 agaccctggt ttcattggcg tcccgggcag gattcgaacc tgcgacctgc cccttaggag
       61 ggggctgcgc tatccatctg tgccaccggg actttgactt tatttatcaa acgcaagtct
      121 acccttatag gcgcaaaatg acaacgggaa agcaaatttc ttatatgaaa atagtgatag
      181 ggctggttgg attgggatgt gttcttagca ggacttatga aaaaccccta ggtcatggca
      241 aaaaatgttt ttaatgaggg aatttagata aactaaatga ccgaattaaa acatttttta
      301 acatagagat tggggttttt cgtaagcact gcttaattat tctgtcattt ctcaattctg
      361 gaaatcggta agtcaatttt tattaaacct ggtgaaggtt ttgtgattgg ctatcaagat
      421 ggctataacc tgataacagc atgacatgaa acaatatcta ttgaccgcat attaataatt
      481 aagatacctt gcgctagttg ataatggatt gcaaaatttg agtcattaaa ggagatgtta
      541 aaatatgaaa atttttaaag gattaaagaa aaacactggt tttacgaagc agtcaaatac
      601 tcaaaaaata tctaattcat cgattcaatt atccattact tctttatctg gtgatgcaa
//
LOCUS       DASLOE010000357          641 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.359, whole genome shotgun sequence.
ACCESSION   DASLOE010000357 DASLOE010000000
VERSION     DASLOE010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.359"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            182..252
                     /locus_tag="U6309_003057"
     tRNA            182..252
                     /locus_tag="U6309_003057"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:214..216,aa:Gly,seq:ccc)
     gene            complement(310..>641)
                     /locus_tag="U6309_003058"
     CDS             complement(310..>641)
                     /locus_tag="U6309_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506448.1"
                     /translation="LKELLYGNEQEGFEELLFQFQREKLAKWTLMTIFQAYFNPEVDV
                     FIKPTTTKNIIKELNLDIVYNPTPSWEFYESYRNIINQMKTKIEKSLAPSNAAFCGFL
                     MMSLDDR"
BASE COUNT          196 a          116 c          112 g          217 t
ORIGIN      
        1 aaagaagcaa ccaagctatt ttccgtgagt tacacttccc atacatttgt atagtttaag
       61 ttaatagttg agttttaaaa cctgttttta aatttaaatc gccgttgtga taacttaatt
      121 gaatcaataa ttataaaatg gtaccttaat attctattga cgaaagggtt cgtgagtttt
      181 tgcgggtgta gttcaatggc agaacctcag cttcccaagc tgatggcgtg ggttcgattc
      241 ccatcacccg ctcaataaaa gaatgtgaca tgtcttcata gagcccatca agcgatactc
      301 atagaaaatt taacgatcat ccaggctcat cattaaaaaa ccacagaatg ccgcattgct
      361 gggcgctagt gatttttcaa tttttgtttt catttggtta atgatattcc tataagattc
      421 atagaactcc caggaagggg ttgggttata tacaatatca aggttcagtt ctttaatgat
      481 atttttggtt gttgtaggtt ttataaacac atccacttca ggattgaagt aagcctgaaa
      541 aatagtcatt aaagtccact tcgccagttt ctctctttga aattgaaaaa gaagctcttc
      601 aaagccttcc tgttcatttc catacaaaag ttcttttaat c
//
LOCUS       DASLOE010000358          641 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.360, whole genome shotgun sequence.
ACCESSION   DASLOE010000358 DASLOE010000000
VERSION     DASLOE010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.360"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            201..440
                     /locus_tag="U6309_003059"
     CDS             201..440
                     /locus_tag="U6309_003059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506449.1"
                     /translation="MQINGLRKWYRLVIMVSIFGLTGCPAVQEPSKQTAATPPTGANA
                     ANQNNTQTANTGNNGAVAPPAPPAATIEITTCINV"
BASE COUNT          208 a          106 c          119 g          208 t
ORIGIN      
        1 ttgggaaaat cataagaacg gcagatctaa ggacttgtgt tgattttcca tgggagtggt
       61 ttaaagtgac agtttaaata attacaaata taataatcgt tgcatctttt aaaaaacgat
      121 atgatatttt ggcgattgcc attggattta ctttaatgta atatactttt tgtttttgaa
      181 ttggcacagg atgttttaat atgcaaatta atggattgcg taaatggtac cgattagtaa
      241 ttatggtttc catattcgga ttgacaggtt gtcctgcagt ccaagaacct tctaaacaga
      301 cagcagcaac tcccccaact ggagcaaatg cagcaaatca aaataacacg cagaccgcta
      361 atacaggtaa taatggggct gttgctccac ctgccccacc tgccgcaacg atagaaatta
      421 ctacttgtat taatgtttaa tattttgtct tatgtgattt ttaattttgt aaaggaatat
      481 aatgaaaatc agaatttgcc tattactctt ggtattcgta gcatcttatt gtcatgcaaa
      541 agataatcct atttcttcca atgaagtgtt aaaaaacaaa caggaacttc agcaggttat
      601 tcaagaatat ctaaagccca ggggagtact ggttagctcc t
//
LOCUS       DASLOE010000359          640 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.361, whole genome shotgun sequence.
ACCESSION   DASLOE010000359 DASLOE010000000
VERSION     DASLOE010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.361"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>640
                     /locus_tag="U6309_003060"
     CDS             <1..>640
                     /locus_tag="U6309_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506450.1"
                     /translation="YVLSFDMLSEEGIDRDNNTFYYTLSVDEICYEIKTSSGCLRTGV
                     FHSNDHNNLEERIFLNAAEKGFVENLEVLKNLATIERLIKELEKKGTEDEIKKYSSEL
                     NHEISLYNDNLRKISNIFPDIDKKIIKKLEINNSILPLPCPFDTRAVQVASHVPNQEG
                     TYRRSQSSNEYHHTNKRGNVITYKNFNELKNNDPNLGEIFLGTDNNSLYVKCK"
BASE COUNT          248 a           86 c          112 g          194 t
ORIGIN      
        1 tatgtattgt cttttgacat gctttctgag gaagggatag atagagataa taacactttt
       61 tattatacgc tctctgtaga tgaaatatgc tatgaaatta aaacatcctc gggatgtttg
      121 cgaacgggag tctttcactc caatgatcac aacaatcttg aggagcggat ttttcttaat
      181 gcagctgaaa aagggtttgt cgaaaattta gaagtcttaa aaaatctagc aacgatagaa
      241 agacttataa aagaattaga aaaaaaagga accgaagacg aaattaagaa atatagctct
      301 gagttaaatc atgaaattag tttatataat gataacttac gaaaaataag caacatattc
      361 cctgatattg ataagaaaat tataaaaaaa ttagaaataa acaatagcat tctgcctttg
      421 ccttgtccat ttgatacacg agcagtacaa gttgctagtc atgttccaaa tcaagaaggc
      481 acttatcgtc gttctcagag ctctaacgag tatcatcata ctaataagag aggtaatgta
      541 ataacttata aaaattttaa tgagttgaaa aataatgacc ccaatttagg ggagattttt
      601 ttaggaactg ataataattc attatatgtg aagtgtaaat
//
LOCUS       DASLOE010000360          636 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.362, whole genome shotgun sequence.
ACCESSION   DASLOE010000360 DASLOE010000000
VERSION     DASLOE010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.362"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..272
                     /locus_tag="U6309_003061"
     CDS             <1..272
                     /locus_tag="U6309_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506451.1"
                     /translation="FITGVQETLATKPPEPEEVHWFKKFLRFITGNEKLLQNADEKRY
                     DQQIKVLSDISDLNKKLNSLPQEKNMPSEHEHSHEHGNENHMKLN"
BASE COUNT          236 a          103 c           98 g          199 t
ORIGIN      
        1 gttttattac tggagtacaa gagacattgg caaccaagcc tcccgagcct gaggaagtcc
       61 actggttcaa aaaatttcta aggtttatca caggtaatga aaaactattg caaaatgctg
      121 atgagaaacg ttatgaccaa caaatcaaag tcctttctga tattagtgat ttaaataaga
      181 aactgaattc tttaccacaa gaaaaaaaca tgccatctga gcatgagcac tctcatgagc
      241 atggaaatga aaaccatatg aagctcaatt agaaaagcta tttttagatt gccagggtag
      301 gtagctttgt ctatatctca gttaagtctg attaatatca aattttataa ttagacttaa
      361 taaagataag agccatgctt tggtagaagg gtaaggatag ctactaaaaa taacccagta
      421 ataattatta ataagatata attgcgctct ttctctttta ttaacgtttt agctatttcc
      481 cataaatcca ttaaagccca aaaaggtcct aaaaaccata agtatattgg tctttcccaa
      541 aaaggtttat aattataacc acctagaata aatagttgtt tgttacataa actacaataa
      601 taaatttgta gatttgtagt tactattctt tcctca
//
LOCUS       DASLOE010000361          629 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.363, whole genome shotgun sequence.
ACCESSION   DASLOE010000361 DASLOE010000000
VERSION     DASLOE010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.363"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..621
                     /locus_tag="U6309_003062"
     CDS             <1..621
                     /locus_tag="U6309_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEN5506452.1"
                     /translation="SDHVIAPGIKNCLLDRDFTYFVDIIHQDSNRVEMVTFASSHQPN
                     STNNFIFNNLDYLNMIAENLSSRARRLHTKDNFLTLPRECIIQMNELISSGNSNKKDN
                     LKEIILSSSQKKMAELIKDNVFDYNQLPFSFLAAKELTHREKEMIYLYFNDFNLSRIA
                     SIFEISKRTVERHFENIKKKLGCENTGQIIPALIKYDCSLKKGIKT"
BASE COUNT          224 a          114 c           98 g          193 t
ORIGIN      
        1 agtgatcatg tcatagcacc aggtataaag aactgcttat tagatcgtga cttcacgtat
       61 tttgttgata tcattcatca ggactccaat cgagttgaaa tggttacctt cgcctcttca
      121 caccaaccga acagcacaaa taattttata tttaataatc ttgattacct gaatatgatt
      181 gctgaaaacc tgtcttcaag agccagaaga ttgcatacaa aagataattt tttaacctta
      241 cccagagaat gcataatcca gatgaatgaa ctgattagct caggaaattc aaataagaaa
      301 gataatctta aagaaattat tttgagctcc tcgcagaaaa aaatggccga actcatcaaa
      361 gataatgtat ttgattataa tcaacttcct tttagttttt tagccgccaa agaactgact
      421 catcgagaaa aggaaatgat ctatttatac tttaatgatt ttaatttgtc ccgtattgcc
      481 tcaatttttg aaatttccaa acgcacggtt gagcgacact ttgagaatat taaaaagaaa
      541 cttggctgtg agaatacagg ccaaataatc cctgcgttga ttaaatatga ttgttcttta
      601 aaaaaaggca tcaagacata acccctact
//
LOCUS       DASLOE010000362          620 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.364, whole genome shotgun sequence.
ACCESSION   DASLOE010000362 DASLOE010000000
VERSION     DASLOE010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.364"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            23..208
                     /locus_tag="U6309_003063"
     CDS             23..208
                     /locus_tag="U6309_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506453.1"
                     /translation="MNTDDINKAYVSPYDKFLYEFDATHKKSASQLQEIKKHQRIFKM
                     RDDKDYKIDQSEIWEEF"
     gene            307..620
                     /locus_tag="U6309_003064"
                     /pseudo
     CDS             307..620
                     /locus_tag="U6309_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213191.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          240 a           95 c           99 g          186 t
ORIGIN      
        1 ataaagttat tttggattga gtatgaacac agatgatata aataaagctt atgtcagtcc
       61 ttacgataag tttttatatg aatttgatgc gacacataaa aaatcagcat cgcaattgca
      121 agaaattaaa aagcatcagc gcatcttcaa aatgagagac gataaagact acaagatcga
      181 tcaaagtgaa atttgggaag agttttaaga gtttctcacc tataactatc ttggtaagct
      241 gctagcatgt taatcaagac ctagtagcag aaatgacagg tctttattgt attagtaaag
      301 ataaagatga aaaatccatc cattctgcac taaataatcc tgctttatta aataaggcta
      361 tgcagcttaa atcaattggg aaagtagccc tttctcaaaa tcaattaact tatcttaaga
      421 ttcatgatga atggatccac gaactatttc cactcttaca aaatgagcag atccgtaaac
      481 ctgattattt tggtaatgaa tccatcggtg ctcatattag tattatctat ccagaagaaa
      541 atacaacaat taatccaaaa gagttagatc aagagcatcg ctttaaaatt aagaatatta
      601 ttaaggcaaa tttagaatta
//
LOCUS       DASLOE010000363          614 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.365, whole genome shotgun sequence.
ACCESSION   DASLOE010000363 DASLOE010000000
VERSION     DASLOE010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.365"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>614
                     /locus_tag="U6309_003065"
     CDS             <1..>614
                     /locus_tag="U6309_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN5506454.1"
                     /translation="TFVKAYAYSPDTSNPIQINRSLAEVNSRHPGEVIVLIHGLMRTY
                     VSMKPLKAYLEAQGYQVYLYKYPSARYNIQEHGVYLNQFITTILENNPAIKIHFVTHS
                     LGGIIVREALSKLSQKQLKHVGYLIMLAPPNQGSKLAKLSIKLFPMISYFIKPLAELS
                     SEQTSYVHRVPVPDIKMGIIAGRFDAKVPPDYARLQGQTEQVII"
BASE COUNT          193 a          124 c          110 g          187 t
ORIGIN      
        1 cacatttgtc aaggcttatg cttactcccc tgataccagt aatccaattc agataaaccg
       61 ttcattagca gaagtaaact ccaggcatcc tggagaggtt attgtattaa ttcatggttt
      121 aatgcgtacc tatgtgagta tgaagccatt aaaagcatat ctggaggctc agggatatca
      181 ggtctatttg tacaaatacc cctccgccag atacaatatt caagagcatg gagtttattt
      241 aaatcaattt attacaacaa tacttgagaa taatccggca ataaaaattc atttcgttac
      301 ccatagcctt ggtggcatta ttgtaagaga agcgttatcc aaattatctc aaaaacaact
      361 aaaacacgta ggttatctca ttatgctggc tcctcctaat caaggttcga agctggcaaa
      421 attatctatt aaactatttc cgatgatttc ctactttata aaacctttgg ctgaattaag
      481 ttctgagcaa acatcttatg tgcaccgtgt tcctgtacct gatatcaaaa tggggataat
      541 cgctgggcgg tttgacgcta aagttcctcc agactatgct cgtctgcaag gacaaacaga
      601 gcaggttatt atta
//
LOCUS       DASLOE010000364          611 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.366, whole genome shotgun sequence.
ACCESSION   DASLOE010000364 DASLOE010000000
VERSION     DASLOE010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.366"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            18..>611
                     /locus_tag="U6309_003066"
     CDS             18..>611
                     /locus_tag="U6309_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN5506455.1"
                     /translation="MFILQGIYMDIKLKIGLRIKESRKNKKFTAVQLAEVTGFSAQRI
                     SNWERGTRTPRFKDAEILGSALGVSPTWLLFLDIDHKPMKDHPFQTIPIINASSKIEG
                     YLPIPSSIQDNITHDDFAFIVHDKSMSPIYNAGDLVTFCKEKQNHCDLVLIHINATEE
                     NLFRKISSEDNTFICSPINPDWPQIRFESASMFQIIGW"
BASE COUNT          217 a          106 c          106 g          182 t
ORIGIN      
        1 gtttataata aacaattatg tttattttgc aaggtattta tatggatata aaattaaaaa
       61 tagggttaag aattaaagag tcgcgaaaaa acaaaaaatt cactgcagtg cagttggctg
      121 aagtcactgg tttttcagcg caacgaatta gtaattggga acgaggaaca agaacgccca
      181 ggtttaaaga tgcagaaata ctcggtagtg cattgggtgt ttctccaaca tggttattat
      241 ttttagatat tgaccacaaa cctatgaagg atcatccctt ccaaacgatc cctataatca
      301 atgcctcatc taaaattgaa ggttacctcc caataccttc atcaattcaa gacaacataa
      361 cgcatgatga ttttgctttt attgtgcacg ataaaagtat gtctcctatt tataatgcag
      421 gtgacctagt tactttctgt aaagaaaaac agaaccactg cgatttagta ctgattcata
      481 ttaacgctac agaagagaac ttatttagaa aaataagctc agaagacaat acgtttatat
      541 gctcacctat caatccggac tggccacaaa ttcgatttga atcagcatca atgtttcaaa
      601 taataggttg g
//
LOCUS       DASLOE010000365          598 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.367, whole genome shotgun sequence.
ACCESSION   DASLOE010000365 DASLOE010000000
VERSION     DASLOE010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.367"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>598
                     /locus_tag="U6309_003067"
     CDS             <1..>598
                     /locus_tag="U6309_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506456.1"
                     /translation="DDAQRNNPFQYIDRKYLSLYRKITRLYAQILGVEQNRGEEKDLA
                     KLTRDLINLQNGVVTSLLKTMPLITDDTSDDEHANKYAQKNFYSPAGMSALFAPLYAA
                     AKVFSYNNEMPISYGSDPYAYFELLLSWNKTLDESQFVRSEVVKEKLENRAFKMLLEN
                     FANESNKTGLVKMGRSEVTICFKNIYTEIYSNQIERKKL"
BASE COUNT          213 a           91 c          114 g          180 t
ORIGIN      
        1 tgatgatgcg cagaggaata acccgtttca atatattgac cgaaaatatc tatctctata
       61 tagaaagatc actcgtcttt acgcgcaaat acttggtgtt gaacagaatc gtggagaaga
      121 aaaagatcta gctaagttaa cgagggattt gatcaatctt caaaatgggg ttgtcacttc
      181 attattgaaa acaatgccct tgattacgga tgataccagt gatgatgagc atgccaataa
      241 atatgcacaa aaaaattttt actcaccagc agggatgagc gctctatttg cgccgcttta
      301 tgcagcagca aaagtgttta gttataataa tgagatgcca atttcttatg gcagtgatcc
      361 ttatgcatac tttgagttat tattatcttg gaataaaacc ttagacgaat cacaatttgt
      421 gcgttctgaa gttgttaaag aaaaacttga aaatcgcgct tttaagatgc tattagagaa
      481 ttttgcaaac gagtcaaata aaactggctt ggtcaaaatg ggaagatctg aagtaacgat
      541 ctgttttaaa aatatttata ctgaaattta tagcaatcaa attgaaagaa aaaaactt
//
LOCUS       DASLOE010000366          597 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.368, whole genome shotgun sequence.
ACCESSION   DASLOE010000366 DASLOE010000000
VERSION     DASLOE010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.368"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..507)
                     /locus_tag="U6309_003068"
     CDS             complement(<1..507)
                     /locus_tag="U6309_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506457.1"
                     /translation="MLILMMEYTFVCKNKQYHLAKECVLHSKIYNLYHSSTELGDLKA
                     AVVKSSRSSKGNMLYHVDLLTNQGSVNLTGSSSSGRDDKEIAANSINNYISNSLETTF
                     KVSYPASWWMYGLSVFFLLLGALLLTVRGAITDFDRILKTIVIKRKGLFKTEETKLSF
                     SDVDKIIVE"
BASE COUNT          182 a          121 c          101 g          193 t
ORIGIN      
        1 ttcgactatt attttatcta catcagaaaa cgataatttg gtttcctctg tcttaaacaa
       61 tccttttctt ttgattacaa ttgtttttaa tattctgtca aaatcagtaa ttgcccctct
      121 aacggtaagc aacagagcac ccagcaatag aaaaaaaacg gataagccat acatccacca
      181 cgaggctgga taactcactt taaaagtcgt ttccaagcta ttgcttatgt agttattgat
      241 gctgttggca gcaatttctt tatcgtcacg cccgctggaa ctgctccccg tcagattgac
      301 tgaaccttgg ttcgttaaca aatccacatg ataaagcata tttcctttac ttgagcgact
      361 gcttttaact acggctgctt ttaaatcccc taactcggtg ctggagtgat ataaattata
      421 aattttgctg tgtaaaacac attccttagc caaatggtat tgtttgtttt tgcaaacgaa
      481 ggtgtattcc atcattaaaa taagcattgt aaaaagactt gctacaagaa acaaaactgc
      541 taacagatat ataggccaca tgctgatgat gaggcgcatc ttgtttgtgc tgataga
//
LOCUS       DASLOE010000367          586 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.369, whole genome shotgun sequence.
ACCESSION   DASLOE010000367 DASLOE010000000
VERSION     DASLOE010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.369"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..373)
                     /locus_tag="U6309_003069"
     CDS             complement(<1..373)
                     /locus_tag="U6309_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEN5506458.1"
                     /translation="MAYHQLSYTFLNHQKLPLIIQPQAKAEGNIDVLKQFLKEENALF
                     KEQLLNYGAIILRGFHVNTAEQFLEVIQSSDLGPNYSYDFCPIPRTQIRENIYTSINI
                     HPSFNLALHNEKSYDQDFPSHI"
BASE COUNT          189 a          109 c          101 g          187 t
ORIGIN      
        1 aaatgtggct gggaaaatcc tggtcatatg atttttcatt atgtaaagca agattaaaac
       61 tcggatggat atttatcgaa gtataaatat tttctctaat ttgtgttcgc ggaatcgggc
      121 aaaaatcata agaataattt ggccccaaat cacttgactg gatcacttct aaaaattgtt
      181 cagccgtatt aacatgaaac ccacgtaaga taatggcgcc ataattgagg agttgctcct
      241 taaacagggc attttcttct ttaagaaatt gttttaaaac atcaatatta ccttccgctt
      301 tagcttgagg ctgaataatt aaaggtaatt tttggtggtt caaaaaagta tagctgagtt
      361 gatgatatgc cataaaattc ctcactgaca atccggaggt gtactatcag actttcagag
      421 tgtaggtcct tcctgattct ataagtggct ccctcttttc acctatatta caaaaattac
      481 cgcatcatct ttgacgaata cctatattaa atagtataga cactgacctt gctatgcaat
      541 gaaaatccac cagattttgc caaagaagct cctgctgaat cactca
//
LOCUS       DASLOE010000368          585 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.370, whole genome shotgun sequence.
ACCESSION   DASLOE010000368 DASLOE010000000
VERSION     DASLOE010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.370"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(156..>585)
                     /locus_tag="U6309_003070"
     CDS             complement(156..>585)
                     /locus_tag="U6309_003070"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN5506459.1"
                     /translation="ITIHPKVGQILLCNFSEGFKEPEMVKDKRPVIVISSALQGRNKL
                     VTVVPISSVKPDPIMPYHYVLPTASLPMLGNFQEAESWVKGDMLYTVAFHRLDLIRLG
                     TRNSQGKRNYYQNRLGREQMKLIYSCVLHGLNIGNLAQHL"
BASE COUNT          184 a          114 c           94 g          193 t
ORIGIN      
        1 caaaggcttc cgaagaagcc taaacatggc ttcaacaagg attgtcatct cctaattgtt
       61 gagccagcta cactgggctg acctttgtgt agtctcgaat gcctgatgct tacgcagagc
      121 aggaacaata taattataaa gtcataattg aaaaattaca aatgctgtgc caaatttcct
      181 atatttaagc catgtaatac acatgaataa attaatttca tttgttctct tccgagcctg
      241 ttttggtaat aatttcgttt accctgagaa tttcttgtgc caagacgtat taaatctaac
      301 ctatgaaaag caacggtata gagcatatct ccttttaccc aactttccgc ctcttggaaa
      361 ttacctaaca ttggcaatga ggctgtaggt aatacataat gatatggcat tatcggatct
      421 ggctttacgc tacttatagg aactactgta actaatttat ttcttccttg taatgcactt
      481 gaaataacaa taactggtct tttatctttt accatttccg gctctttaaa accttccgaa
      541 aaattacata aaagaatttg tccaactttg ggatgaatag taata
//
LOCUS       DASLOE010000369          584 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.371, whole genome shotgun sequence.
ACCESSION   DASLOE010000369 DASLOE010000000
VERSION     DASLOE010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.371"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..448
                     /locus_tag="U6309_003071"
     CDS             <1..448
                     /locus_tag="U6309_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEN5506460.1"
                     /translation="ILEILQANCEINNQELADLVALSPSSCLRRVKLLIDNGYIKKQV
                     ALLEPEKVGLKLTVIVLVGLNNHQPEVMNQFEKTIQLFPEVIQCYLITGQSADYLLKI
                     IVPDLNAYQAFLLGKLTRINGVASVHSSFILRNVSDTTALPLDHLD"
BASE COUNT          192 a          106 c           97 g          189 t
ORIGIN      
        1 aatacttgaa atactgcaag caaattgcga aatcaataac caggaattgg ccgaccttgt
       61 ggccttgtct ccttcgtcct gtttgcgtcg agtcaaatta ttaatagata atggttatat
      121 caagaaacag gttgccctgc ttgagccaga aaaagtaggg ttaaaattga ctgttattgt
      181 tttagtaggg ttaaacaatc accagcctga ggttatgaat caatttgaaa aaaccataca
      241 actttttccg gaagtcattc aatgctactt gattacaggc caatctgctg attatttatt
      301 gaagataatt gtgcctgatt taaacgctta tcaagctttt ttattaggca aattaacacg
      361 catcaatggc gttgcaagtg ttcactccag ctttatatta cgcaatgttt ctgacacgac
      421 cgccctaccc cttgatcatt tggattaggg cactgctctt cgaatattaa attattcaat
      481 aaatactaac tcgaaagtac ctggctctgc ctataaaatc aaaaattcta taaaaataga
      541 taaaaataga acaaatttat taatttaatg cttattttta tagg
//
LOCUS       DASLOE010000370          566 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.372, whole genome shotgun sequence.
ACCESSION   DASLOE010000370 DASLOE010000000
VERSION     DASLOE010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.372"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>566
                     /locus_tag="U6309_003072"
     CDS             <1..>566
                     /locus_tag="U6309_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN5506461.1"
                     /translation="LVSKGISKTEPIPKHLEQINQWAAGIDIGATSHFVAVPEGCTTT
                     AVREFKSFTPDLYSLAEWLKECGIQTIAMESTGVYWIPVYELLEEKGFEVKLVDARRV
                     KNVSGRKTDVLDCQWIQQLHTYGLLNGAFRPDNEICALRSYVRQRSMLIRSASRYIQQ
                     MQKALTQMNLQLHHVITDITGDTGMRIIR"
BASE COUNT          192 a           89 c          127 g          158 t
ORIGIN      
        1 ctggtgtcaa agggaatatc taaaacggaa ccgataccca agcatctcga acaaattaat
       61 caatgggcag ctggaattga tataggtgcc acatctcatt ttgtagctgt acctgaagga
      121 tgtacaacaa cagcagttcg tgaatttaag agttttactc cagaccttta ttcattggcg
      181 gagtggttaa aagagtgtgg aatacaaacc attgcaatgg agtcgacggg ggtttattgg
      241 atccctgttt atgaattatt agaagaaaaa ggatttgaag ttaaattagt tgatgcaaga
      301 cgagtaaaaa atgtgagtgg tcgaaaaaca gatgtattag attgccaatg gatacaacaa
      361 ctacatacct atggtttatt aaatggggca tttcgtccgg ataacgagat atgtgctctt
      421 cgctcgtacg ttagacaacg tagcatgtta ataagaagtg catcaaggta tattcaacaa
      481 atgcaaaaag cactcactca aatgaatctt caattgcatc atgtgattac agatattact
      541 ggtgatacgg ggatgagaat tattcg
//
LOCUS       DASLOE010000371          562 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.373, whole genome shotgun sequence.
ACCESSION   DASLOE010000371 DASLOE010000000
VERSION     DASLOE010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.373"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
BASE COUNT          187 a          106 c          102 g          167 t
ORIGIN      
        1 ttatgctcta taattaaaca gtggcgctcc gccttatgtt atttcacttt gagaaaattg
       61 ctattggaac agggcaataa agctcaaaac ctaggattta ctagggaagt aactgaagga
      121 gtacttgatg ctacatagca aaaaaaccgt ttttgtaagg caacattgga gaaagctata
      181 cagcgatccc atttatgttc ggtcttactg gcggtcacct cctagtaggt aaactctact
      241 aaaggcattt gcgccaccca tttcgattcg ctaaaagtaa aataatgagg caaaaaatca
      301 aacaaataag tttgtttttg cttacagaca aaaatgcagt accccataat tgcccctgaa
      361 tatataatac aaactcaaat attgagtttt atcatatata tatgaactca attcttctga
      421 ttaatattca aaaggttcgc ggttcgattc cgcgcagcgg caccaggtat accaatggtt
      481 ataggttgtt ttcaaaatca ccatccggat tatgggacac atatgggaca gttgtagata
      541 actatttacc tttagtaact ag
//
LOCUS       DASLOE010000372          555 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.374, whole genome shotgun sequence.
ACCESSION   DASLOE010000372 DASLOE010000000
VERSION     DASLOE010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.374"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>555
                     /gene="istA"
                     /locus_tag="U6309_003073"
     CDS             <1..>555
                     /gene="istA"
                     /locus_tag="U6309_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEN5506462.1"
                     /translation="TLRSKKEEVIVRFETEPGQQMQVDWAQFRRGKDRLSAFIATMGY
                     SRASYVEFVSDETLETLIACHKNAFEYFGGVPYTILYDNMKTVIIERNGYGPSQHRFQ
                     AGFWDFAKHTGFRPKVCQPYRAQTKGKVERFIHYLKYSFYFPLVGQLKALGLSLDKET
                     ANMHVLKWLNEIANQRVHATTGAIP"
BASE COUNT          185 a           87 c          118 g          165 t
ORIGIN      
        1 actttaaggt caaaaaaaga agaggttatt gttcggtttg aaacagagcc tggacagcag
       61 atgcaggtgg attgggctca attccgaaga ggaaaggata gattaagtgc ttttattgca
      121 accatgggat attccagagc atcttatgta gagtttgtca gcgatgaaac cttggaaacg
      181 cttattgcat gccataaaaa tgcattcgaa tattttggtg gagttcctta tacgatccta
      241 tatgacaata tgaaaacagt cattattgaa cgaaatggat atggcccaag ccaacatcga
      301 tttcaagctg gtttttggga ttttgcaaaa catactggat ttcgtcctaa agtgtgtcaa
      361 ccttataggg cacaaacaaa aggtaaagtt gaacgcttta ttcattattt aaaatacagt
      421 ttttattttc ctttagttgg gcaattgaag gcacttggct taagtttaga taaagagaca
      481 gccaatatgc atgttttaaa atggctaaat gaaattgcta atcaacgagt acatgctaca
      541 acaggagcaa ttcca
//
LOCUS       DASLOE010000373          537 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.375, whole genome shotgun sequence.
ACCESSION   DASLOE010000373 DASLOE010000000
VERSION     DASLOE010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.375"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>537
                     /locus_tag="U6309_003074"
     CDS             <1..>537
                     /locus_tag="U6309_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lpg2745 family Dot/Icm T4SS effector"
                     /protein_id="HEN5506463.1"
                     /translation="LLELFVLINMAQSYDVDEQVKAIMSWLYQFNPILKASNKGLLSF
                     YAELEPKFHSEGHLEKRVETGTDNLLYRIKTFLVSLFVTPFEVFPFSGKTISFWDIKN
                     VIFSEGQEIYNQFTPFIMTNKLDALIAVYKKTMDEHIIPCQKNKHICKWLTHYQSTLD
                     WYQLVEAGDLSKMDVYWF"
BASE COUNT          181 a           78 c           99 g          179 t
ORIGIN      
        1 atctattaga actttttgtt ttaatcaata tggcgcaatc ttatgatgta gatgaacagg
       61 taaaagctat aatgagttgg ctgtatcaat ttaatcccat attaaaggca tcaaataaag
      121 ggttattatc tttttatgca gaattggagc caaaattcca ctcggaaggt cacttagaga
      181 agagagtaga aactgggaca gataatttat tgtatcgtat taaaactttt ttagtgtcat
      241 tatttgttac cccattcgaa gtttttcctt tttcaggtaa aacaatcagt ttttgggata
      301 tcaaaaatgt aatcttttca gaaggacaag agatctataa tcagtttact cctttcataa
      361 tgacgaacaa gttagacgct ttaattgcag tctataaaaa gacaatggat gagcacatta
      421 tcccttgcca aaaaaataaa cacatttgta aatggctgac acactatcaa tcaacgctgg
      481 attggtatca gctggtagag gcgggtgatt tatctaaaat ggatgtttat tggtttg
//
LOCUS       DASLOE010000374          535 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.376, whole genome shotgun sequence.
ACCESSION   DASLOE010000374 DASLOE010000000
VERSION     DASLOE010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.376"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>535)
                     /locus_tag="U6309_003075"
     CDS             complement(<1..>535)
                     /locus_tag="U6309_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEN5506464.1"
                     /translation="GQDNEGATIKFSLAIANAASGLQTTDGDAITLTLESGFVVGRDA
                     GGDAVFAIAIDADTGVLSLAQYESIKHPSGGASYDEAVDLSGKINAVVTVTDGDGDVA
                     TQAIGIGDAIVFEDDGPVAQIAATGVKVTHDETAGVDAGSDDVASPLSVFVGVVNKST
                     DMAGFAQSSGAVVSSAGS"
BASE COUNT          121 a          175 c          119 g          120 t
ORIGIN      
        1 actgcccgca gaactcacca cagcaccact gctttgcgca aagcctgcca tgtccgtaga
       61 cttgttgacc acacccacaa acaccgacaa aggactggcc acgtcgtcgc tgccggcatc
      121 cacaccggcg gtttcatcgt gagtcacttt gacacccgtg gcggctattt gagcaacagg
      181 cccgtcgtct tcaaaaacaa tcgcatcgcc tatgcctatc gcttgtgtgg ccacatcccc
      241 atcgccgtcg gtcactgtca ccaccgcgtt gatcttgccg gacaaatcca cggcttcgtc
      301 ataagaagcc ccacctgatg ggtgtttaat agattcgtat tgagccaggc ttaagacccc
      361 ggtatcggca tcaatggcaa tcgcaaacac cgcatcgccg cctgcgtctc gtccaactac
      421 aaaaccgctt tcaagcgtca aggtgatggc atccccgtcc gtggtttgta atccgctcgc
      481 cgcattggct atcgctaaag aaaatttaat ggtagcccct tcgttgtctt gacca
//
LOCUS       DASLOE010000375          510 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.377, whole genome shotgun sequence.
ACCESSION   DASLOE010000375 DASLOE010000000
VERSION     DASLOE010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.377"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(277..501)
                     /locus_tag="U6309_003076"
                     /pseudo
     CDS             complement(277..501)
                     /locus_tag="U6309_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          175 a           88 c           79 g          168 t
ORIGIN      
        1 tctgaaagtt ttcagtaaag accgccgcta tttctcggtt gcttgcaaca aaatattggc
       61 ttcactaatc cattgcaaac caaataaaaa attatatggc cgcaacaatg tgcttgctag
      121 agtagacaat aagaaatggg ttgcccatgt tattgaaatt ttatctgatg aaaaagaagt
      181 caaatatcgt ttaacaatga attaaatagc ttgtaatttt ttctttcagc caaaaaacat
      241 atatttaata atagtttccc ttcatgctac gtaatcacta atctgcattg agcctaactg
      301 tctgatattc aaaaaatcac agataaaacg tgctgctgga aaacctcctc ttgtaacagc
      361 gacaactaca tcaaattgat atgccgattg ggtaataact tttgtcagtt tataactgat
      421 gccataaact tctcgcagta ttattatttt tgcggaaaat cttgtttcat cgtaataaac
      481 tggttgatag attaatatca ttagagagtt
//
LOCUS       DASLOE010000376          507 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.378, whole genome shotgun sequence.
ACCESSION   DASLOE010000376 DASLOE010000000
VERSION     DASLOE010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.378"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(282..500)
                     /locus_tag="U6309_003077"
     CDS             complement(282..500)
                     /locus_tag="U6309_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506465.1"
                     /translation="MHGYGRLKKVIALILSVGPIFILASCAISSKADNVLQADEQLAT
                     DEHQKHKGHGGYGGGRGVVLEDVRSGRK"
BASE COUNT          186 a          113 c           59 g          149 t
ORIGIN      
        1 ttgctaacaa agtcaggaac attataatgt aattcccaga ttaaaaaagg cattataaac
       61 agactagaga aaaagaagac taaaaaaata atcaaacaaa ttattcccat tacagatagt
      121 ttgctgttgt tatcatacca actatgctcc ataggctcct tctataaaat ggccagctaa
      181 ctaaaattat tcttttttta aattttaaga catcagcatc catcatataa ccgaatatat
      241 tttaagctag atattaaaaa atagcttaac gacaaataaa cttatttccg gccgcttcta
      301 acgtcctcca acaccacccc acgaccacct ccatatcccc catgcccttt atgcttttgg
      361 tgctcatctg tagctaattg ctcatcagcc tgtaaaacat tatctgcctt tgatgatata
      421 gcacaagaag ccaaaataaa tattgggccc actgataata ttaaagcaat aactttcttc
      481 aatcttccat aaccatgtat cgcaaaa
//
LOCUS       DASLOE010000377          497 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.379, whole genome shotgun sequence.
ACCESSION   DASLOE010000377 DASLOE010000000
VERSION     DASLOE010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.379"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>497
                     /locus_tag="U6309_003078"
     CDS             <1..>497
                     /locus_tag="U6309_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEN5506466.1"
                     /translation="TKTQDITNTIERLFTNSQDSIIVFNQQGKIIAINEKAAALFKKS
                     PDKLIDKPIWKLFKLNSFAIKRQFIQAKKCFLLANEGIAQQFTWLESKAQKPVLAYHI
                     LFNKTEIEDTSIIFTKLTDILKSKTIEWILWSLTKISNHQEINEIIDQILQLTSDVFA
                     ADYTSV"
BASE COUNT          198 a           91 c           66 g          142 t
ORIGIN      
        1 acaaaaactc aagacattac caacacgatt gaaagattat ttacaaactc tcaagacagc
       61 atcattgttt ttaatcaaca aggcaaaatt attgctatta atgaaaaagc tgctgcttta
      121 tttaaaaaaa gccctgacaa actaattgac aaaccgattt ggaaactatt taagttaaat
      181 agcttcgcca ttaagaggca atttattcaa gcaaagaagt gttttctttt agccaatgaa
      241 gggatagccc agcaattcac ttggcttgaa agcaaagcac aaaaaccagt acttgcatac
      301 catatcctct tcaataaaac agaaatagaa gacacatcaa tcatttttac caaacttacc
      361 gatattctaa aatcgaaaac catagaatgg attttatggt ctttaactaa aataagtaac
      421 caccaagaaa tcaatgaaat tattgatcaa attctacaat taaccagtga tgtttttgca
      481 gcggattata cttctgt
//
LOCUS       DASLOE010000378          491 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.380, whole genome shotgun sequence.
ACCESSION   DASLOE010000378 DASLOE010000000
VERSION     DASLOE010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.380"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>491)
                     /locus_tag="U6309_003079"
     CDS             complement(<1..>491)
                     /locus_tag="U6309_003079"
                     /inference="COORDINATES: protein motif:HMM:NF014069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN5506467.1"
                     /translation="FSHSLGACYIGKLVCDQLDINNGDRKIIMGAALLHDIGHGPFSH
                     AFESIFKGFGEGKISHDKQWTPKFIDSFECDLKEDILNVFIDPNHHEYYDPLIASQLD
                     VDRLDYLLRDSHFCGVPYGNIDLQWIISCMKQQEINGDTVICITPKGIGAIEHYILSR
                     RLMT"
BASE COUNT          171 a          102 c           70 g          148 t
ORIGIN      
        1 gtcatcaatc ttcttgacaa aatataatgc tcaattgctc cgataccttt aggagtaata
       61 caaattactg tgtcaccatt gatttcttgt tgtttcatac aggatataat ccattgcaag
      121 tcaatattgc cgtatggaac tccgcaaaaa tgactgtctc gcagcaaata atctaatcta
      181 tctacatcta actgtgaggc aatcaatggg tcataatact catgatgatt aggatcaata
      241 aaaacattaa gaatatcttc ctttaagtca cattcaaagg aatcaataaa cttaggagtc
      301 cattgtttat catgtgatat ttttccctca ccgaaccctt taaaaatgga ctcaaaagcg
      361 tgagaaaaag gcccatggcc aatatcatgc agtaaagcag cccccattat aatttttcta
      421 tcaccattat ttatgtctaa ttggtcacaa accaacttac ctatataaca agccccaaga
      481 gaatggctaa a
//
LOCUS       DASLOE010000379          473 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.382, whole genome shotgun sequence.
ACCESSION   DASLOE010000379 DASLOE010000000
VERSION     DASLOE010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.382"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>473
                     /gene="tuf"
                     /locus_tag="U6309_003080"
     CDS             <1..>473
                     /gene="tuf"
                     /locus_tag="U6309_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946066.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN5506468.1"
                     /translation="VESGIVKVGEEVEIVGIRDTQKTTCTGVEMFRKLLDEGRAGDNV
                     GVLLRGTKRDEVERGQVLAKPGTIKPHTKFEAEVYVLSKEEGGRHTPFFNGYRPQFYF
                     RTTDVTGTCDLPSGVEMVMPGDNVQLVVSLHAPIAMDEGLRFAIREGGRTVGAGVV"
BASE COUNT          136 a           75 c          137 g          125 t
ORIGIN      
        1 gttgagagtg gaattgttaa agttggtgag gaagttgaaa ttgttggaat aagagacacc
       61 caaaagacga cttgtacggg tgttgagatg ttccgtaaat tacttgatga aggtcgagct
      121 ggtgataacg ttggtgtgtt attacgaggt acgaagcgag atgaagtgga gcgtggacag
      181 gtattggcta agccaggtac catcaagcca cacaccaagt ttgaagcaga agtgtatgta
      241 ttatccaagg aagaaggcgg acgtcacaca ccattcttta atggataccg tccacaattc
      301 tatttcagaa ccactgacgt aacaggtact tgtgacttgc catcaggagt tgaaatggta
      361 atgcctggag ataatgtgca attagttgtt agcttgcatg ctccgattgc gatggatgaa
      421 ggtttaagat tcgcaattag agagggtggc cgaactgttg gcgccggtgt agt
//
LOCUS       DASLOE010000380          470 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.383, whole genome shotgun sequence.
ACCESSION   DASLOE010000380 DASLOE010000000
VERSION     DASLOE010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.383"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..>470)
                     /locus_tag="U6309_003081"
     CDS             complement(<1..>470)
                     /locus_tag="U6309_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEN5506469.1"
                     /translation="AISGEALAVTILPIALWSVTNHGLLIIWMIFTYIFSDLYKSMLL
                     FYYNKQPTLFSTEKWLFLFNLGVVSSGLAWGFASSIMIPASSTLHQIFVVFLITGVTA
                     AANSLYSPVRLSYFLFLVTAFVPFTIWLFSKGQVYLLLRFCGIIYMGIMLFISY"
BASE COUNT          198 a           78 c           79 g          115 t
ORIGIN      
        1 aatatgaaat aaacaacata atccccatat aaataatgcc acaaaaccgc aacaataaat
       61 aaacttgtcc ttttgaaaat aaccatatag tgaaaggaac aaaagcagtg acaagaaata
      121 aaaaatagga taatcgcaca ggtgaataga gtgaattggc tgctgctgta actcctgtaa
      181 taagaaaaac gacaaagatt tgatgcaatg tacttgaggc tggaatcata attgaactgg
      241 caaatcccca tgccaagcca gaacttacaa cacccaagtt aaaaaggaat aaccattttt
      301 cggttgaaaa caacgttggt tgtttattgt aatagaagag aagcatgctt ttataaagat
      361 ctgaaaaaat ataagtaaaa atcatccaaa tgataagtag cccatgattt gtaacagacc
      421 ataatgcaat aggtagtata gttactgcta aagcttcacc tgatatagcc
//
LOCUS       DASLOE010000381          458 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.384, whole genome shotgun sequence.
ACCESSION   DASLOE010000381 DASLOE010000000
VERSION     DASLOE010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.384"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(25..237)
                     /locus_tag="U6309_003082"
     CDS             complement(25..237)
                     /locus_tag="U6309_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506470.1"
                     /translation="MKSTNNMQKKETDKPTSAQAQKKEVRRNNAPDFIPGASPYYNLY
                     CFFYKLCHGEDTSTQHQPNTTIQHGR"
BASE COUNT          132 a           63 c           98 g          165 t
ORIGIN      
        1 aatctagtta tgtcaaaaag aaatttatct cccgtgctga atagtagtgt ttggttgatg
       61 ttgtgtggat gtgtcttcgc catggcaaag tttataaaaa aaacaataaa gattataata
      121 aggtgatgca ccagggataa aatcgggggc attatttctt ctcacttcct tcttttgtgc
      181 ttgtgccgaa gttggtttgt cagtctcttt tttttgcata ttatttgttg atttcataaa
      241 tcttatcctt gattaagaaa atagattgaa ttttaagtat cgttcaaacc aggaaagaga
      301 gttccggctt gtttacaaat gaaccgtttt attgctcatg gcttggtgat tatagttcag
      361 acaggtgatc aattgtagta gttgtcccag atgtgttatg gttatctgac tgacaggaat
      421 cgagatgcca gaacaaggaa tagataatga actgtttt
//
LOCUS       DASLOE010000382          458 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.385, whole genome shotgun sequence.
ACCESSION   DASLOE010000382 DASLOE010000000
VERSION     DASLOE010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.385"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(14..>458)
                     /locus_tag="U6309_003083"
     CDS             complement(14..>458)
                     /locus_tag="U6309_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEN5506471.1"
                     /translation="GIYHGCLLAVYHVFRFYYIKRNLQTENQLMKIGSIIMTYSLVVI
                     GWVIFRSTSFEQAFTILGKMYDIPGFVQEFAMNQRASAYTDSLFYLVGMFALCFIGPT
                     FKSIETKILSAVQPFWIKKWLYSLSIFTVLIFMFSPVKPFIYFVF"
BASE COUNT          185 a           81 c           69 g          123 t
ORIGIN      
        1 acaaattatc taattaaaaa acaaaataaa taaacggttt cacaggggaa aacataaaaa
       61 ttaacaccgt aaaaattgaa agcgaataaa gccatttttt aatccagaat ggctgtacag
      121 ctgacaggat tttcgtttca attgatttaa aagttggccc tataaaacac agggcgaaca
      181 ttcccacgag ataaaacaat gaatcggtat aagctgaagc acgttgattc attgcaaatt
      241 cctgcacgaa accaggaata tcatacatct taccgagaat agtaaatgcc tgttcaaatg
      301 aagtagatcg aaatattacc cagcctatga ctactaatga ataagtcatt ataattgagc
      361 caatcttcat caattgattt tcagtttgta aatttctttt aatataataa aaccggaaaa
      421 catgataaac ggctaacaaa caaccatggt atatgccc
//
LOCUS       DASLOE010000383          452 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.386, whole genome shotgun sequence.
ACCESSION   DASLOE010000383 DASLOE010000000
VERSION     DASLOE010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.386"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            44..>452
                     /locus_tag="U6309_003084"
     CDS             44..>452
                     /locus_tag="U6309_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5506472.1"
                     /translation="MIKDILNTIASLIEENAEVFAGESKKQLLQKVKADLASFKPINF
                     YALDAEKQIKGALTLIESYCIEMNRILDELNHQSSVSLANEKLINKIKNILTNLKNKQ
                     NKINLLREKIPEGMLDVKRALYIAENSFLSESEP"
BASE COUNT          183 a           71 c           71 g          127 t
ORIGIN      
        1 tctattcaat ctaagcccat tctattgagt aagagagtac taaatgataa aggacattct
       61 caataccata gccagtctta ttgaggaaaa cgcagaggta tttgccggtg aatctaaaaa
      121 acaactttta cagaaggtta aagccgatct tgcatcgttt aaaccgatta atttttatgc
      181 attggatgca gaaaaacaaa taaagggcgc gcttacacta atagaaagct attgcattga
      241 aatgaacaga atattagatg aattaaatca tcaatcttca gtttctttag ccaatgaaaa
      301 attaattaat aaaataaaaa acattttaac taacttaaaa aacaaacaaa ataaaatcaa
      361 cctgttacga gaaaaaattc cagagggtat gcttgatgtc aagcgagcat tgtatattgc
      421 agaaaattct tttcttagcg aatcagaacc ag
//
LOCUS       DASLOE010000384          448 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.387, whole genome shotgun sequence.
ACCESSION   DASLOE010000384 DASLOE010000000
VERSION     DASLOE010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.387"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..240)
                     /locus_tag="U6309_003085"
     CDS             complement(<1..240)
                     /locus_tag="U6309_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013101793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HEN5506473.1"
                     /translation="MRKAYVSVNGIKAGILEELQSGKYQFSYFDDYHGAPVSLTMPLT
                     NKVYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND"
     gene            complement(237..>448)
                     /locus_tag="U6309_003086"
     CDS             complement(237..>448)
                     /locus_tag="U6309_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN5506474.1"
                     /translation="ANLVHYYRKQSGLSQQELAHLAGVGKTVIYDIEKGKESVRLNTL
                     LKVLDVLNIQMKFETPFPQTTDNNS"
BASE COUNT          131 a           85 c           78 g          154 t
ORIGIN      
        1 atcattttta tcgattttat atttgcgtaa tagtgcttca agcataatgc cttcaggaag
       61 taacccttca aaaaaagggg gaaatacatc aaaatcatag accttattgg ttaacggcat
      121 tgtgagagaa acaggtgccc cgtgataatc atcaaagtaa ctgaattgat attttccgct
      181 ttgtaattct tctaatattc cagccttgat gccattcact gatacgtatg cttttctcat
      241 gaattattat ccgttgtttg aggaaaaggc gtttcaaatt tcatttggat attcaaaaca
      301 tctaatactt ttagcaaggt gttcaaccgc accgattctt tccctttttc aatatcatat
      361 ataactgtct tgccgacacc cgctaagtgg gctaactctt gttgtgataa gccgctttgt
      421 ttacggtagt aatggactaa attggcaa
//
LOCUS       DASLOE010000385          445 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.388, whole genome shotgun sequence.
ACCESSION   DASLOE010000385 DASLOE010000000
VERSION     DASLOE010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.388"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            246..>445
                     /locus_tag="U6309_003087"
     CDS             246..>445
                     /locus_tag="U6309_003087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506475.1"
                     /translation="MELDKYCAIILDLLFEQHANSSSQKNISLGINEIKREIKPKLGR
                     EYKVQNFNHALDYLLQNELIVK"
BASE COUNT          154 a           61 c           86 g          144 t
ORIGIN      
        1 tataaatcaa tgagttattg gtggaggcgg cggctattta atttattggt caagatgttg
       61 tattttaagg gttatttttg tgggcgataa aaatataccc ccaaatgtac ccccagcctg
      121 atgttgtagc tctatttttt tatcctctga aaaggaatgt tttatatcgc aggctcattc
      181 agcgcagctt agtctaaaat aataatatta gtgcgtaaac catgtatgga acggtaggga
      241 tcaaaatgga attagataaa tactgtgcaa taattttaga cttacttttt gagcagcatg
      301 caaactcctc aagccaaaaa aatatttctt taggaattaa tgaaattaaa cgagaaataa
      361 aaccaaaatt aggtagggag tataaggtac agaatttcaa tcacgcttta gattatttgc
      421 tacaaaatga attgattgtt aagga
//
LOCUS       DASLOE010000386          443 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.389, whole genome shotgun sequence.
ACCESSION   DASLOE010000386 DASLOE010000000
VERSION     DASLOE010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.389"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>443
                     /locus_tag="U6309_003088"
     CDS             <1..>443
                     /locus_tag="U6309_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4917 family protein"
                     /protein_id="HEN5506476.1"
                     /translation="HLDLFNLHDFDFSSLPHDDGFTRDMNTNDFPKWNNNLSRGPQDV
                     FYLHGSLFIINAIENFIKINNDSKDGRILEQIDKWLKDDQQPLIVLEGNYKDKAKKIY
                     RHSYLRCCLNALSTLKGDLFLIGFSINDETDKHIIESIQESVIERV"
BASE COUNT          157 a           63 c           77 g          146 t
ORIGIN      
        1 catttagacc tgttcaattt acatgatttt gacttttcat cattaccaca tgacgatgga
       61 tttacgcgtg atatgaatac caatgatttt ccaaaatgga ataataatct ttcaaggggt
      121 ccgcaagatg ttttttactt acatggaagc ttattcatta ttaatgctat agaaaatttt
      181 attaagatta ataacgatag caaagacggg cgcattttag aacagataga taaatggttg
      241 aaggatgacc agcaaccatt aattgtttta gagggtaatt ataaagataa ggctaaaaaa
      301 atttatagac actcatacct tcgatgttgt ctaaatgctt tatctacttt aaagggtgat
      361 ctatttttaa tcggcttttc aataaacgat gaaacggaca aacacatcat tgaaagcatt
      421 caagaatctg tgattgaaag ggt
//
LOCUS       DASLOE010000387          443 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.390, whole genome shotgun sequence.
ACCESSION   DASLOE010000387 DASLOE010000000
VERSION     DASLOE010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.390"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            278..354
                     /locus_tag="U6309_003089"
     tRNA            278..354
                     /locus_tag="U6309_003089"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:312..314,aa:Pro,seq:ggg)
BASE COUNT          120 a           97 c           83 g          143 t
ORIGIN      
        1 cgggtacaat gacaccaatg tccaatcggc cttctttttc atgagcccat cgtgtgagat
       61 tctacataaa atatttaaag ttatttatag atgtggagca ctcgtgcttt aaatgcttac
      121 gaaaaacccc aatctctatg ttaaaaaatg tttttaattc ggtcatttag tttatctaaa
      181 ctctctcatt aaaaacattt tttgccttga ccttggagtt tttcataagt cctgcccttg
      241 ttattttagc cttatttaga tatcatgcct atccaatcgg ggcgtagcgc agcctggtag
      301 cgcactagca tggggtgcta gtggtcgaag gttcaaatcc ttccgtcccg accaagccca
      361 gcaagggttt tataagattc tccaaacccc agtagagata tttggtacca gttttggtac
      421 cgatgcaaaa tgacactcta agc
//
LOCUS       DASLOE010000388          439 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.391, whole genome shotgun sequence.
ACCESSION   DASLOE010000388 DASLOE010000000
VERSION     DASLOE010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.391"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..>439
                     /locus_tag="U6309_003090"
     CDS             <1..>439
                     /locus_tag="U6309_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014843835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEN5506477.1"
                     /translation="ISVFYANWNINQKRAYKDLKEVAINLSNNIDGFIEDLLQEIYAL
                     PVYGKKIVDCKSDLYPFLEHITLNNPKISGVIISDNKHNLFCSTLPNNKDLIATPIRS
                     RSIIGPYNLPLFDQPVYLIQQKMGNYLIGILVIASVLKSELQTD"
BASE COUNT          153 a           74 c           61 g          151 t
ORIGIN      
        1 atttcagttt tttacgctaa ttggaatatt aaccaaaaaa gagcttataa agatttaaaa
       61 gaagttgcaa tcaatctcag taacaacata gatggtttta tcgaagatct acttcaggaa
      121 atctatgctt tgcctgtata tgggaaaaaa attgtcgatt gtaaatcaga tttatatcca
      181 ttcttggaac atatcacact gaataatcct aaaatctctg gtgttattat tagtgacaac
      241 aagcacaatc tattttgttc aactttacct aataataaag atcttattgc aacaccgatt
      301 cgttcacgtt ctattatagg cccctataat ttaccgttat ttgaccaacc cgtttatctg
      361 atccagcaaa aaatgggcaa ttaccttata ggtattttag ttattgcttc tgtcttaaaa
      421 agtgaattac aaacagata
//
LOCUS       DASLOE010000389          435 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.392, whole genome shotgun sequence.
ACCESSION   DASLOE010000389 DASLOE010000000
VERSION     DASLOE010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.392"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..317
                     /locus_tag="U6309_003091"
     CDS             <1..317
                     /locus_tag="U6309_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEN5506478.1"
                     /translation="VFAKNLGKYTYEIACKNCHAPNLAKAIKAPPAFDKKAWKLRFKQ
                     AKIEAKNNPSQFESAMDYLLYNVKVGKGLMHHGGLCKEADVPNTDCSDEALIEAINYM
                     RK"
BASE COUNT          156 a           76 c           76 g          127 t
ORIGIN      
        1 cagtttttgc taaaaattta ggaaaatata cttacgaaat tgcctgtaaa aattgccatg
       61 cccctaattt agccaaggct ataaaagccc ctccagcttt tgataaaaag gcatggaagc
      121 tgcgatttaa acaagcaaaa atagaagcaa aaaataatcc ttcacaattt gaaagcgcaa
      181 tggattacct gctgtacaat gtcaaggtgg gtaaagggtt gatgcatcat ggcggtttat
      241 gtaaagaggc agatgtccct aataccgatt gctctgatga ggcattaatt gaagcgatta
      301 attacatgcg aaaataagat aaaatattta aatacatacc agcttttggc tgggtttcat
      361 ttgtaaataa actattgttt cctttcaatg aacattaata ttcagcaaca taaccccaat
      421 cttttaccca tgtaa
//
LOCUS       DASLOE010000390          424 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.393, whole genome shotgun sequence.
ACCESSION   DASLOE010000390 DASLOE010000000
VERSION     DASLOE010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.393"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            49..>424
                     /locus_tag="U6309_003092"
     CDS             49..>424
                     /locus_tag="U6309_003092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506479.1"
                     /translation="MDVASFKKFITEYSDCSNDVINSNFNNYIHNLNKYLSLINNNID
                     ISNFLKTKLPSVDFATLYESGLDKTGGMGACSKIDWPLNKYELLSTQKELFEYIAAEK
                     IKPHEYCLNFIGSFNNLNHSVEQ"
BASE COUNT          155 a           57 c           68 g          144 t
ORIGIN      
        1 gtgtcgaata tgtgtcgaat aatgaagtga tatttaagga tatttgaaat ggatgttgcg
       61 agttttaaga agtttataac cgaatattct gattgttcca acgatgtaat taactcaaat
      121 ttcaataact acattcataa tttgaacaaa tatttatctt taattaataa caatatcgat
      181 atatccaatt ttctaaaaac taaactacct agtgttgact ttgcaacatt atatgaatcg
      241 ggattggata agactggcgg tatgggggca tgtagtaaaa tagattggcc attaaataaa
      301 tacgagttgc tttccactca aaaagaacta tttgaatata ttgctgctga aaaaattaaa
      361 ccgcatgaat attgcctcaa ttttattggt tccttcaata atcttaatca cagtgtagaa
      421 caaa
//
LOCUS       DASLOE010000391          416 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.394, whole genome shotgun sequence.
ACCESSION   DASLOE010000391 DASLOE010000000
VERSION     DASLOE010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.394"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..386
                     /locus_tag="U6309_003093"
     CDS             <1..386
                     /locus_tag="U6309_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN5506480.1"
                     /translation="LPFFEQYNLPILRILTDRGTEYCGKVEHHDYQLYLAINNIDHTK
                     TKANSPQTNGICERFHKTILQEFYQVTFRKKIYESIDELQKDLDEWMDYYNNQRTHQG
                     KMCCGRTPMQTLIEDKQIWMEKFIN"
BASE COUNT          161 a           70 c           71 g          114 t
ORIGIN      
        1 tcttaccatt ctttgagcaa tacaacttgc ctatactgcg aattttaact gacagaggca
       61 cggaatattg tggaaaggta gagcaccatg attatcagct ttatttggcg attaataata
      121 tcgaccatac gaagacaaaa gcaaactcac ctcaaacaaa tggaatttgt gagcgtttcc
      181 ataaaacaat tttacaagag ttctatcaag taacatttcg aaagaaaatt tacgaatcaa
      241 tcgatgaatt acaaaaagat ctggacgaat ggatggatta ctataataat caacgaactc
      301 atcaaggtaa aatgtgttgt ggtcgtactc caatgcaaac tttgatagag gataaacaaa
      361 tctggatgga aaaatttata aactaaattt gacctgacag acacttcttg aaaaac
//
LOCUS       DASLOE010000392          405 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.396, whole genome shotgun sequence.
ACCESSION   DASLOE010000392 DASLOE010000000
VERSION     DASLOE010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.396"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..405)
                     /locus_tag="U6309_003094"
     CDS             complement(<1..405)
                     /locus_tag="U6309_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system protein LssZ"
                     /protein_id="HEN5506481.1"
                     /translation="MHILAKVIHCIFPLIALTLLIIGIKRNAIYYIISSLWVSLISLV
                     IHYQTSGGEILGTYFNYLNATIYTINLFVLCLSLVYVLWHLSNDNVAFKIVSSLFQSF
                     IVIGALLVVINLWINAYFIENRLIGTPVMQVAL"
BASE COUNT          168 a           68 c           60 g          109 t
ORIGIN      
        1 aagagctacc tgcatcacag gagttcctat caacctgttt tcgataaaat aagcgttaat
       61 ccaaagattg attacaacca ataaagctcc aatgactata aatgattgga acaaactgct
      121 aacaatttta aacgctacat tatcattact taagtgccat aaaacataaa ccaaagataa
      181 acacaaaacg aacaaattga tggtatatat tgttgcgttg agataattaa aataagtacc
      241 taaaatctct ccaccggaag tctgataatg aatgactaat gaaataaggc ttacccataa
      301 cgaagaaata atataataaa tcgcgtttcg tttgatccca ataattaaaa gtgttagggc
      361 aattaatgga aagatacagt gaataacttt agccaagata tgcat
//
LOCUS       DASLOE010000393          399 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.397, whole genome shotgun sequence.
ACCESSION   DASLOE010000393 DASLOE010000000
VERSION     DASLOE010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.397"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            2..>399
                     /locus_tag="U6309_003095"
     CDS             2..>399
                     /locus_tag="U6309_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEN5506482.1"
                     /translation="MTHDAFLDDLILSIVQTESIAEQSDLQNSLRKRGYEIPQATLSR
                     RLKKMKIAKVSGYYKIIDYNQPGLPLVLNVQISDFGLIILQTHPGNANSLAYYIDRKY
                     VSFSIKDSSNSGILGTIAGDDTVLLIIKSKS"
BASE COUNT          130 a           73 c           72 g          124 t
ORIGIN      
        1 attgacccat gatgcctttt tggatgattt gattttatcg atagtgcaaa cagaaagtat
       61 tgcggaacaa agtgatttgc agaacagctt aagaaaaaga gggtatgaga ttcctcaagc
      121 aactctttct cgtcgcttaa aaaaaatgaa aattgcaaaa gtatccggtt attacaaaat
      181 cattgattac aatcagcctg gcttacctct ggttctaaat gtccagattt ctgattttgg
      241 gctaatcatt ctgcaaactc atcctggtaa tgcaaacagt cttgcttatt atatagaccg
      301 taaatacgtt tccttcagca taaaagactc ttcgaattca ggaattttag gaactattgc
      361 cggagacgac acggtcttgc tcattattaa aagtaaatc
//
LOCUS       DASLOE010000394          390 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.398, whole genome shotgun sequence.
ACCESSION   DASLOE010000394 DASLOE010000000
VERSION     DASLOE010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.398"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            10..351
                     /locus_tag="U6309_003096"
     CDS             10..351
                     /locus_tag="U6309_003096"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEN5506483.1"
                     /translation="MTSDLSSKHCESCEGIGAALNSEQIKNLLPQLNTKWEVTEDNRI
                     IKRAFSFKNFYETMAFVNAIAWIANIENHHPDLEVGYNYCRVHFMTHALNGLTHNDFI
                     CAAKIDKLLVD"
BASE COUNT          136 a           62 c           70 g          122 t
ORIGIN      
        1 ggaatcaaaa tgactagtga tttaagtagt aagcattgtg aatcttgcga gggaattggc
       61 gctgcgctca actccgagca aatcaaaaat ttactacctc aattaaacac aaaatgggaa
      121 gtaacagagg acaaccgaat tatcaagcgt gctttttcat ttaaaaattt ttatgaaacg
      181 atggcttttg tcaatgcgat tgcctggata gcaaatattg aaaatcatca ccctgattta
      241 gaagtcggct acaattattg ccgtgttcac tttatgacgc atgcacttaa tggtttaact
      301 cataatgatt ttatttgcgc agcaaaaata gataagttac tggttgatta aaaattattg
      361 actcttttta agaagataca ttttaacaaa
//
LOCUS       DASLOE010000395          388 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.399, whole genome shotgun sequence.
ACCESSION   DASLOE010000395 DASLOE010000000
VERSION     DASLOE010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.399"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            148..>388
                     /locus_tag="U6309_003097"
     CDS             148..>388
                     /locus_tag="U6309_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506484.1"
                     /translation="MKLKSLLFVVCFSLFSNVFAANLHLHPKADNADKKSIQKSVTYP
                     GYCQIEIINQSFTDVRVFGTFDDGTTIDFNIYRYES"
BASE COUNT          122 a           63 c           59 g          144 t
ORIGIN      
        1 atttttaata ttttcatagt tgtttctgct tcttctctaa caaaattgaa ttgtaatgaa
       61 ctttgatttt atgggtacaa aaaaatccta ttgactaaat tgaatacttt gatactatgc
      121 agcttccatc ttactaaggg gaagctcatg aagttaaaat cattgttatt tgttgtatgt
      181 ttcagtttat tttctaacgt ttttgctgca aatcttcact tacatcccaa ggctgataat
      241 gcagacaaga aatcaataca aaagagcgta acttatcctg gttactgcca aattgaaatc
      301 attaaccaaa gcttcacaga tgttcgtgta ttcggtacgt ttgatgatgg cacaactata
      361 gattttaata tctatcgtta tgagtcac
//
LOCUS       DASLOE010000396          369 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.400, whole genome shotgun sequence.
ACCESSION   DASLOE010000396 DASLOE010000000
VERSION     DASLOE010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.400"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            159..>369
                     /locus_tag="U6309_003098"
     CDS             159..>369
                     /locus_tag="U6309_003098"
                     /inference="COORDINATES: protein motif:HMM:NF012575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEN5506485.1"
                     /translation="MINKKHILNNRYFCKNDENYFIVKLNNKRFAIPDKCPHRGGPLS
                     LGNICRESQRIQCPWHDGYFKISSLI"
BASE COUNT          136 a           57 c           59 g          117 t
ORIGIN      
        1 ggtgagttaa ttttctatcc caatagccta agtcctatta tattaaaggc taatcataga
       61 attgtaatac ctaaagggcg gttacattcc actgtttcgc aatctgcaaa tactcaatat
      121 gtagttcagc caattgaaat ttccacagga gcataaaaat gattaataaa aaacatatct
      181 taaataatag gtatttttgt aagaatgatg aaaattattt cattgtgaaa ttaaataata
      241 aacgttttgc aataccagat aaatgtcctc acagaggcgg tccattaagc ctaggaaata
      301 tatgccgaga atcacaaagg atccaatgtc cttggcatga tgggtatttt aagatttctt
      361 cattaataa
//
LOCUS       DASLOE010000397          352 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.402, whole genome shotgun sequence.
ACCESSION   DASLOE010000397 DASLOE010000000
VERSION     DASLOE010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.402"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            complement(<1..296)
                     /locus_tag="U6309_003099"
     CDS             complement(<1..296)
                     /locus_tag="U6309_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEN5506486.1"
                     /translation="MKLLGKILLVFTALMIFVSITLWILATNIKPETIKQLVSNKITA
                     ITHKQSQIDGTISWQLFPRPGLKFNKIHIGNENLNDNYSLLIDTLLLNLKITPL"
BASE COUNT          125 a           47 c           62 g          118 t
ORIGIN      
        1 aacaaaggag taatttttaa atttaatagc aatgtatcaa tcagaagaga ataattatca
       61 tttaaatttt cattacctat atgtatcttg ttgaatttga gcccaggcct tgggaacaat
      121 tgccaggaaa tagtcccatc aatttggctt tgcttatgag tgattgctgt gattttatta
      181 ctaaccaatt gcttaatggt ttcgggttta atatttgttg ctaaaatcca taaagtgatt
      241 gatacaaaga tcattaaagc agtaaaaacg agtagaattt ttcctagcaa tttcatggga
      301 tagaaccaga gtttaaaaac caaagcatat agataaagga gtttctgtca ag
//
LOCUS       DASLOE010000398          350 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.403, whole genome shotgun sequence.
ACCESSION   DASLOE010000398 DASLOE010000000
VERSION     DASLOE010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.403"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            27..>350
                     /locus_tag="U6309_003100"
     CDS             27..>350
                     /locus_tag="U6309_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506487.1"
                     /translation="MDNRRKGMSQFNFFEKNQAFDENNFEQKLQDAINLIKQNQSFVA
                     RLVLDAINSNQVTVSTFFQLTPDHYQRMKTIMKKDYHTTLPKTFPPTMSAVRKIEKEL
                     DGIIYD"
BASE COUNT          135 a           59 c           56 g          100 t
ORIGIN      
        1 aaattgcatg gaagtaatca ttaatactgg ataataggag aaaaggaatg tctcaattca
       61 atttttttga aaaaaaccag gcttttgatg aaaacaattt cgaacaaaaa ttgcaggatg
      121 caatcaatct gataaaacaa aatcaatctt ttgttgcaag attagtgctc gatgcgataa
      181 attccaatca agtcacagta agtactttct ttcaacttac cccagatcat taccagagaa
      241 tgaaaaccat tatgaaaaaa gattatcata ctactcttcc aaaaactttc ccgcctacaa
      301 tgtcggcggt gcgtaaaatt gagaaagaac tggatggcat tatttatgat
//
LOCUS       DASLOE010000399          338 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.404, whole genome shotgun sequence.
ACCESSION   DASLOE010000399 DASLOE010000000
VERSION     DASLOE010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.404"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..258
                     /locus_tag="U6309_003101"
     CDS             <1..258
                     /locus_tag="U6309_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506488.1"
                     /translation="AYKITLSSKGLVCTHSEIEENRMIDLLKEIKNDDSQAIFWIFKQ
                     FDKQPKEQLGIIDCTQNRIYYHYSGEVEKIDEAIEKLSKKI"
BASE COUNT          133 a           48 c           56 g          101 t
ORIGIN      
        1 gcctataaaa ttactctttc ttcaaaaggg ctggtttgta cccattctga aattgaagaa
       61 aacaggatga ttgatctgtt aaaagaaata aaaaacgatg attcccaagc tattttctgg
      121 atctttaaac aatttgataa gcagcctaaa gaacaactag ggatcatcga ttgtacccaa
      181 aatcgtattt attaccatta ttcaggcgaa gttgaaaaaa ttgatgaggc tattgaaaaa
      241 ctaagtaaaa aaatataagc ctaaaagtgc ccaaagaaat atcgcatttg attgtgtgga
      301 aatatatttc aaatcctgga ttgaaaatat attatatg
//
LOCUS       DASLOE010000400          334 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.406, whole genome shotgun sequence.
ACCESSION   DASLOE010000400 DASLOE010000000
VERSION     DASLOE010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.406"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
BASE COUNT          110 a           61 c           47 g          116 t
ORIGIN      
        1 cattaggcaa acgctcaatc aacctcaaat actatgttat ctctcaatac acaaaaacat
       61 gctttggcta acttatgatc cactgtttta atctgaaact ccgttatacc tcgattcaaa
      121 tggcgagtgg ctcagcgtac atgtagagca accgctttat atcaaaaagc ctacttttca
      181 tagtcttttg tcgcatttgc gacgatttac atcattttac tttaatcatt cgttcataaa
      241 ataaaaacca aatgatcata aagtgatcaa ttaagctaag aattgatatt gaagtttttg
      301 aggttgtttt gattatgaaa tctattgaat attt
//
LOCUS       DASLOE010000401          332 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.407, whole genome shotgun sequence.
ACCESSION   DASLOE010000401 DASLOE010000000
VERSION     DASLOE010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.407"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
BASE COUNT          118 a           53 c           57 g          104 t
ORIGIN      
        1 attttttaaa tcaaatcgaa cgcatgtaat tgagctttat tgttatagtg atgaatatgc
       61 gcaacaagtt aatcatgaga taacttcagg tgcagattct ggcccacttt taacaaaaat
      121 atatggccaa gatgttagat ttatctatgc tcctgattcg gaaaaattta atctggtttt
      181 aaacgaggcc aggaaaagaa attataacca accaataatc aatctttatg aaccagataa
      241 tattaaatac ttattatctc gattaagtca tggcgattcc atacttatta acgggcaggg
      301 tgatattcat aagcaactaa ttgcgggacg ca
//
LOCUS       DASLOE010000402          327 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.denovo.408, whole genome shotgun sequence.
ACCESSION   DASLOE010000402 DASLOE010000000
VERSION     DASLOE010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.denovo.408"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
BASE COUNT          111 a           62 c           51 g          103 t
ORIGIN      
        1 gctaacgtta atagacctga gctgcaattt cgctggcatc tcttaaatta tgatgaacta
       61 cataaaaaaa aatgggaaga caaattcgat ttgattatca gtaatccacc ctattttcaa
      121 ccaggtcacg gaatgctttc cccttcaaaa tttaaaaatc ggtgcagatt ttatttagat
      181 agctcttttc agagttatat tcaagcgctg ggcaattcac ttgcaaacag aggtaaagct
      241 tattttttat tacgtccatt aaaacaccat ggtcttgatt tattttctga tatacaaaaa
      301 atcctgcagg aaacatcagt aacagca
//
LOCUS       DASLOE010000403        12386 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.guided.085, whole genome shotgun sequence.
ACCESSION   DASLOE010000403 DASLOE010000000
VERSION     DASLOE010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 12386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12386
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.guided.085"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            <1..1009
                     /locus_tag="U6309_003102"
     CDS             <1..1009
                     /locus_tag="U6309_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Dot"
                     /protein_id="HEN5506489.1"
                     /translation="LREWFPTQLVNNSVVKTEMLEGEKGVFNAFKNYKEYRSFMPKEE
                     LKTTEFGVLYEANEAFLEYFYNRSESTLINKDSPFEKYFVDSSSDEEAIKNYWDAEGY
                     ILYCQHLAKPQVKLSYIEIMTMMKVINQPITIYDRSTSSVVAEYVNPKVNLPDFEVAI
                     DALQGHYFLLKTEETEKELEEYERSYAQYKRDRSEILAHSDKPVSSLLVRATCPKGHL
                     DEDPFIALIESLSEINSLSQIDTNLKNENTDTPNCNFLLKVGASVVGTSSALITLMGI
                     AILSGQYDPGNSLSEEALATTVVSSIVGLGYGLYSFFSSSPKVSELDSTNGLSQKLKM
                     N"
     gene            1204..1623
                     /locus_tag="U6309_003103"
     CDS             1204..1623
                     /locus_tag="U6309_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEN5506490.1"
                     /translation="MSAKKNSIAHDRLLTEVELELMNIIWSLDKVTIKEVVSHLPKER
                     PLAYTTVATVLKVLEQKGFLECQKNSYAHVFMPIVTKSDYENTCIDHMVANVFDGEPR
                     ALVQRLLLARKLQHDDIQAIEEALKQLNAGEKQPEVM"
     gene            1623..3533
                     /locus_tag="U6309_003104"
     CDS             1623..3533
                     /locus_tag="U6309_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HEN5506491.1"
                     /translation="MLLIEIVLTIHCLLLVSSLFIGRAWLANKPSFKLKLLRFLLASC
                     VISPIIVHCFNFDQKPERQNYVSFDVLQQYVKQPIFKTESSKFSHESSSAFTSMNVNY
                     CQLLSVMFFLIVLFRSYHLLSGLGRLKLMLAEAIPYRTSGKLVIKVSHHCHVPFSILL
                     FNKAYIVLPVSIFSSSMNVRIAIAHEGQHHRNGDCSWAYFIEIIRIIFFGNPGVTQWF
                     RVLADLQELSCDEVLVGHQKISAHDYGHCLLQVVEAVSQCSLASNREITCTVGMALNK
                     ENEDCKFIIRRISMLTAYSPNASRPLLLGIVFAGFSILSPICVAYSAAGSLTSSRAKE
                     VDISHLDPRMQQIAEKQITTAVKQYHAKSGVIAIADPQTGNIIAFAESSKNKGLESWK
                     SRIFSPGSTIKPFIAAAAINSGNSSETKNYDCHSPYYIAGKTFTNYNSNVESASLAEA
                     IAKSINVCLIRVSQEAGVSVIRKKLTEFGFDTSTWWQADRSDDLQLAMAALGENIPVT
                     IESLIKSYAILANKGHSLDRGNSAIISETTTYSINHMLENAVTNGTGKLAVIPGVSVA
                     GKTGTVIENNDKYLALFAGYVPADNPRYVVLVVIEEGYFSKNGKTLVSGGELAAPVFR
                     NVAMDALSSANR"
     gene            3583..4383
                     /gene="blaOXA"
                     /locus_tag="U6309_003105"
     CDS             3583..4383
                     /gene="blaOXA"
                     /locus_tag="U6309_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014845076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HEN5506492.1"
                     /translation="MKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQR
                     YSPASTFKIALSLMGFDSGILKDELHPELPYKKEYELYLNVWKYPQNPHTWIRDSCVW
                     YSQALTRQLGMKRFKGYVDAFHYGNQDVSGDKGQNNGLTHAWLSSSLSISPTEQIQFL
                     QKIIYKKLPVSQKAYTMTKNIMYIQELPGGWKLYGKTGTGRQLTKDKSQKLPLQHGWF
                     VGWIEKDERVITFAKHIADSKENTTFASFRAKNDTLIQLFNLINELEK"
     gene            complement(4443..5318)
                     /locus_tag="U6309_003106"
     CDS             complement(4443..5318)
                     /locus_tag="U6309_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011215465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEN5506493.1"
                     /translation="MDKSPKLMHLLVYAVHLVLVSTCCFGEPNSPEKLLKITIANKTY
                     TLSELKKQFKTIRVIISHNPAYNYQKKTYDAFDINQILEQLMGADLQKNNADQVLLAQ
                     TLDKYVSQTPLLHFTTKGHAYLAYQETPGTISDNNITKDGRWSYINQHGKKENPGPFY
                     IVWDNTSTYPVSWPYQVISMQIVNKKELTFSHFLNPLNESASVQNGHHIFNNICSTCH
                     SLFYKGALGRAPDLGMVTSYLTPSDISKLVKNGRGYMPPIGKNLSTEEINDLIKFLIW
                     VNKQASKLKCEINSQ"
     gene            5682..7142
                     /locus_tag="U6309_003107"
     CDS             5682..7142
                     /locus_tag="U6309_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN5506494.1"
                     /translation="MNHKPIQFKDLSLIYPHKTCFQDFSGEIHFADRIALIGRNGSGK
                     STLLKMLCGLFLAPAGEIKIPHDVRFGHLPQVLEDCPDLSGGQRLNRVLSKILSDEPN
                     VLLLDEPTNHLDRRNRRSLMRMLEHYPGTLIIASHDTELINTVTDTLWHIDLGQVTVF
                     RGIYFDYQQILEDKKASIEQELSRIARQKKESHLALMKEQERNKRARVQGEKKIAQRK
                     WPTIRSHTKLGSAITTGNKRLSHINYKKQQLLEELSSLNFQEVIKPKFWLKGLGHHKP
                     LIRIQDASAAYEAVVILEDIHFQLSGCERVALHGNNASGKSTFVKAILGESQIKRTGE
                     WAVPDCNTIGYIDQHYQHLNFNETVLDLIRSSMPHASHTEIRAHLNDFLFRKNEEVEI
                     KVKNLSGGEKARLSMALIAANPPKLIILDEITNNVDLETRTHIIQVLREFQGAMLVIS
                     HDYDFLESIHIETRYQIHQGKIHHFNEKQLEVVHHD"
     gene            7135..8916
                     /locus_tag="U6309_003108"
     CDS             7135..8916
                     /locus_tag="U6309_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN5506495.1"
                     /translation="MTNRLPNHLGSFMWHFLKPYQGIVILFILFALLAGFWGPFNSLL
                     IKSFINTLAEKTSQDLSSLYWIAGLLVLNFIVFDNITWRTLGYLNYKYEAVIKNQIIS
                     QTFEYVLGGSTQFFQDNLSGRIADQITTLADNLEVILHRVSVDFLRGASLLVVSFITA
                     YFVNVLFFYILFLWFVAFASFSIWMSARLVQLSDDHASSESQLSGQLVDSLANQSNIR
                     IFSRKIYEVKRMNTFFRLVQQAFQRKELFIVLLCCAQGGMIAVMMGLASITLIYLYGK
                     GLVSIGDFALILGLSMELGHMMWYTMYQVDQFNQALGKARQSLNALVIPHDIKDKSNA
                     SQLVVTQGRIEFSKVKFHYQGGYSLFQNKSVTIESGQKVGLVGYSGSGKSTFVNLILR
                     LYEVVDGQILIDGQNLAEVTQESLRQAIAMIPQDPTLFHRSLMDNIRYGRTDASDEEV
                     ISASKKAHAHEFISLLPEGYKTLVGEHGVKLSGGQRQRIAIARAILKNAPILMLDEAT
                     SQLDSITESNIQESLWDLMQGKTTLVVAHRLSTLLHMDRILVFDKGHIIEDGTHTELL
                     NRGGLYKTLWDAQVGGFLPDQQNEKGE"
     gene            8920..9876
                     /locus_tag="U6309_003109"
     CDS             8920..9876
                     /locus_tag="U6309_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014844018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5506496.1"
                     /translation="MSVKQEQCLIKVVSYDSNWPVQFEQEAERIKKVLGSNCIEIHHI
                     GSTSVPDLVAKPVIDMIPVVLDISKVDGANAAMRALGYEAKGEYGIPFRRYFQKGDNR
                     RTHHAHIFELGNSEIDRHLKFRDWMRMHPKDREAYARLKQELARQYSDDITAYCLGKE
                     DFIATIDKKAGFNGLRMVKALTTREWDKVRHFRQFYFFGKAGLSDPYTWTFEHNAHVH
                     FVLFQGSEITGYAHLQLWSHNRAALRIIVIDELKRNHQYGTRFLALCEKWLKSQGYQS
                     LHVESSPDALKFYRNNGYIDMPFDDPDGYEGDPRDTAVGKIL"
     gene            9873..10400
                     /locus_tag="U6309_003110"
     CDS             9873..10400
                     /locus_tag="U6309_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011946599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN5506497.1"
                     /translation="MIQIEIVIKTLELSDIPLLVDAFQKANWEKPASLFEAYYQEQRK
                     SLRIVWLAYSQDQIAGYVTLKWISQYQPFAHQKIPEIMDLNVLPSFRKQGVGTALLQA
                     AEEKAAGESDVVGIGVGLYGGHDGGYGQAQRLYVNRGYIPDGLGVTYGYKSTVPGQSY
                     PLDDDFILWFTKKLR"
     gene            10466..11218
                     /locus_tag="U6309_003111"
     CDS             10466..11218
                     /locus_tag="U6309_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN5506498.1"
                     /translation="MAYTVKQLAKISNVSVRTLHWYDEIGLLKPAYQGANGYRYYEEK
                     QCLKLQQILFFRELGFPLNDIQKLLMQDDFDHIKALRAHKQVLESDINRKNQLIVTID
                     KTIQHLRGKHKMSNEELYYGFDSLRQKQYEKELVKEGVVSQDWMNNYKKKTSQWTQAD
                     KDRFIQEGKDINQALISAINNQLKPASPEVQAIIHRHHAWVGWNPSKEGYIGLSQRYL
                     TPEFRKFYEWQHPDLLEFIIEGMLFYANKNLD"
     gene            11470..>12386
                     /locus_tag="U6309_003112"
     CDS             11470..>12386
                     /locus_tag="U6309_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN5506499.1"
                     /translation="MTQWFVKDLSQLTGVSVQTLHHYDRIGLLKPSLRLANGYRVYSE
                     KDLLKLQQIIALKFFGFELSQIKTLLAEEGNALNHFNSQARVLEQKASTLLEGAKTLR
                     RIIDSVDKNQSIPWETIIQLIEVYKMTEHLEHGWVKEIFTPDELKQYAEFEKEMKANA
                     TPEQKAAMENNWSQLVDEVKNNLKQDPDSKIVIYLGKKIMDWVNGVYGKKYAHLRTKK
                     FEQGFGEGKGLDEVGLTPEIVSWMDKAMDAYWRDRIYGILDQVGKAPSASILDLWNEM
                     LVDMYGEETSRKNAIYEIALNDDQVSPEA"
BASE COUNT         3892 a         2163 c         2533 g         3798 t
ORIGIN      
        1 gcttagggaa tggttcccaa ctcaacttgt taataattca gtagttaaaa cagaaatgct
       61 ggaaggtgag aaaggagtat tcaatgcttt taaaaattat aaagagtatc gctcatttat
      121 gccgaaggaa gaattaaaaa caacggaatt tggtgtgtta tatgaggcga acgaggcttt
      181 tttagagtat ttttataatc gctctgaaag tactttaata aataaggatt caccttttga
      241 aaaatatttt gttgactcgt catcggatga agaagcaatt aaaaattatt gggacgcaga
      301 agggtatata ttatattgtc aacatttggc gaaacctcag gttaaattat cttatattga
      361 aattatgacc atgatgaagg tcattaatca gccaattaca atatatgatc gttctacttc
      421 ctcagttgta gccgaatatg tcaacccaaa ggtcaattta cctgattttg aagtggctat
      481 tgatgctctt caaggacatt attttctttt aaaaacagag gaaacggaaa aagagttaga
      541 agaatatgaa agaagttatg ctcaatataa aagagataga agtgaaatcc ttgcacactc
      601 cgataaacct gtttcttcct tattggttcg agctacttgc cccaaaggtc atttggatga
      661 agatcctttt attgcattaa ttgaaagcct ttccgaaata aacagcctta gccaaattga
      721 tactaatttg aaaaacgaga atacagatac ccccaattgc aacttccttt taaaggtagg
      781 tgcttctgta gttggtacct cttcagcact aataacttta atggggatag ccatattatc
      841 tggccaatat gatcccggaa acagtctgtc tgaagaggca ttagctacta ctgttgtatc
      901 cagtattgtc ggtttaggtt atgggcttta tagcttcttt agttctagcc ctaaggtatc
      961 agagcttgat tctaccaatg gattatcgca aaaacttaaa atgaactaac ctcctgaatt
     1021 agcaaatatt gtctgtaagc cgatcgtgtt gatattagag gtatcaacac gattgcgcca
     1081 ttcaagtaaa ccgttctcgc gtgtagagaa gtatttttcc catgactatt tcaactctac
     1141 tatttttata gtagaatcct actataaaaa tagtagagac ttatgtggtt attggagaga
     1201 aacatgtcgg caaaaaaaaa ttcgatagct catgatcgac ttcttacaga agtagagctt
     1261 gaattaatga atattatctg gagccttgat aaggtaacta ttaaggaggt tgtttcacat
     1321 ctgcctaagg aaaggccttt ggcttataca accgtggcaa cagtgctaaa agtgctggaa
     1381 caaaagggat ttcttgagtg tcaaaaaaat tcctatgctc atgtttttat gcccatagtt
     1441 acaaagtcag attatgaaaa cacttgtatt gatcatatgg ttgccaatgt ttttgatgga
     1501 gagccccgag cacttgtcca aagactttta ttagccagaa agttacagca tgatgacatt
     1561 caagccattg aagaagcact taaacaatta aacgctggtg aaaaacagcc agaggtgatg
     1621 taatgttatt aatagaaatc gtattgacta ttcattgcct gcttcttgtc agttcactat
     1681 ttatagggcg tgcctggtta gcaaataaac catcttttaa actgaaatta ctccgttttt
     1741 tattggccag ttgcgttatt tcaccaataa tagttcattg ttttaatttt gatcaaaagc
     1801 ctgaacgaca aaactatgta tcatttgatg ttttgcaaca gtatgtaaag caacccatat
     1861 ttaaaacaga atcttcaaaa tttagtcatg aatcgtcttc tgcctttaca tctatgaatg
     1921 ttaactactg tcaactctta agcgtgatgt tttttttaat cgtcttgttt cgtagttatc
     1981 acctgttatc aggtttaggt cgcttgaaat tgatgttggc tgaagcaatc ccatatcgaa
     2041 cctccggaaa actagttatt aaggtttctc accactgcca tgttcctttc tcaatattgc
     2101 tatttaataa agcctacatc gtattgccag tatccatatt ttcttcttcc atgaatgtga
     2161 ggattgctat tgctcatgaa gggcaacacc atcgtaatgg agattgttca tgggcctatt
     2221 ttatcgaaat catacgcatt atcttcttcg gaaacccggg ggtaactcaa tggtttcgtg
     2281 ttttagcaga tttacaagaa ttgtcatgtg atgaagtatt ggttggacat caaaaaattt
     2341 cggcacatga ctatggtcac tgtctattgc aagtggtaga ggcagtatcc caatgctctc
     2401 tggcatctaa ccgggaaatt acatgcacgg tgggtatggc tttgaacaaa gaaaacgaag
     2461 actgcaaatt tataataagg agaatttcaa tgctaactgc ttattcaccc aatgcatcca
     2521 ggcctttgct attagggata gtctttgcag gattttctat cctgtcacca atctgtgttg
     2581 cctattcagc agcaggatct ttgacgagtt ccagggcaaa agaagttgat atttctcatt
     2641 tagatcccag aatgcagcag attgcagaga aacaaattac gacagccgtc aaacaatatc
     2701 atgcaaaatc tggagttatt gcaatagctg atccccaaac aggcaatatc attgcatttg
     2761 ctgaatcaag taaaaataag gggttagaga gttggaaatc acgcattttt tcacccggat
     2821 ctactataaa accttttatt gcagcagctg caatcaactc tgggaatagc tctgaaacca
     2881 aaaattatga ttgtcattca ccttattaca tagcaggcaa aacatttaca aattataatt
     2941 ccaatgttga atcagcttcc ttagcagaag ccatcgcaaa atctatcaat gtatgcctta
     3001 tcagagtttc tcaagaagca ggagtgtcgg taatccgtaa aaagctcact gaatttggtt
     3061 ttgacacaag cacttggtgg caagcagatc gaagtgatga tttacaactg gcaatggctg
     3121 cacttggaga gaatatccct gtcacaatcg aatcgctaat aaaatcttat gccattcttg
     3181 ctaacaaagg acactcactt gacagaggaa atagcgccat tatttcagaa acaaccacct
     3241 actcaataaa tcatatgctt gaaaatgctg ttacaaatgg cactggaaaa ctcgcagtta
     3301 ttcctggtgt ttctgtagct ggtaagacag gaactgtaat tgaaaataat gataaatatc
     3361 ttgctttgtt tgccgggtat gtgccagctg ataatccgcg ttatgttgta ttagtcgtga
     3421 ttgaagaggg ttatttcagt aaaaatggaa agacattagt ttcgggagga gaattggccg
     3481 caccggtatt ccgaaatgtc gcaatggatg ctctgagcag tgcaaatcga taactagctt
     3541 atgggtttgc atatccatat attcaagaat gttgaggttg ttatgaagaa aataattata
     3601 tttttgtgca caggattatt attttgcgtc tctacttggg ctcaaggcac ctgtttttta
     3661 gcaaaagaaa atcaaactgt tttaaaacgt gagggcaatg attgtgatca acgctattcg
     3721 ccagcatcaa cttttaaaat tgcccttagc ctcatgggct ttgattctgg aatattaaag
     3781 gatgagttgc atcctgaatt gccatacaaa aaagaatatg aactttatct gaatgtatgg
     3841 aagtaccctc aaaacccgca tacctggatc agagactcct gtgtttggta ttcacaggca
     3901 ttaactcgac aattgggcat gaaacgcttt aaagggtatg tcgacgcatt tcactacggt
     3961 aatcaggatg tttcgggaga caaaggtcaa aataacggtt taacccatgc ctggctttca
     4021 agctcacttt ctatctcacc cacagagcaa attcaatttc tacaaaaaat aatctataag
     4081 aaactaccgg tgagtcaaaa agcttatacc atgactaaaa atatcatgta catccaggag
     4141 ttgcctggtg gatggaagct ttatggaaaa acaggtactg gtcgacagtt aacaaaagat
     4201 aaaagtcaaa aattgccttt acaacacgga tggttcgtag gttggattga aaaggatgag
     4261 cgtgtgatta cctttgcaaa acatattgca gacagtaaag agaataccac ttttgccagt
     4321 tttagagcga aaaatgatac attaattcaa ttgtttaacc tgattaatga actggagaaa
     4381 taatccaaaa gatggccggt tcagtgtttt ctgatccggc tatctaattc cctgaattat
     4441 aatcactggc tgttaatttc gcattttagt ttggatgctt gtttattaac ccagattaag
     4501 aatttaatga gatcattaat ttcttccgta gagaggtttt tgcctatcgg tggcatgtat
     4561 cctcgcccat tctttacaag tttactgata tctgaaggcg ttaaataact cgtcaccata
     4621 cctaagtctg gtgctcgtcc tagagccccc ttgtaaaaaa gagagtggca tgtggagcat
     4681 atattattaa aaatatgatg accattttgc acagaagcag attcatttaa tggatttaaa
     4741 aaatgagaaa aagtcagctc tttcttattt actatttgca tcgaaataac ctggtatggc
     4801 caactcacag ggtaggtact tgtattatcc catacaatat agaaaggacc aggattttct
     4861 tttttaccgt gttgattaat gtaactccaa cgaccgtctt tggtgatatt attgtcagag
     4921 attgttccgg gcgtctcttg ataagcaaga taggcgtgcc cctttgtcgt aaaatgcaac
     4981 aagggagttt gagacacata tttatctagt gtttgagcta ataatacctg gtcagcattg
     5041 tttttttgta aatctgctcc catcaattgt tctaaaatct gatttatgtc aaacgcatcg
     5101 tatgtttttt tctgataatt atatgcagga ttatgagaga taatcacacg aatggtttta
     5161 aattgttttt ttagctccga cagggtatat gttttattgg caatagtaat tttaagtagt
     5221 ttttctggtg aattgggttc tccaaaacaa catgttgaga ctaataccag gtgaaccgca
     5281 taaaccagaa gatgcattaa ttttgggctc ttgtccataa gtccttcttg tttgttgaaa
     5341 acaaatcaga tttttcccat cctgttacta gctattatgg tttataacat ggacacttcc
     5401 aagtgatatt ttccgacatg cagactcatt tatctcaaac gagaggcgct aataagctac
     5461 caggagaact cgataagcca ggaaaaataa ttttgaatat aatattgaca tcttctacta
     5521 ttttaattta tatttcatgt aattttttta ccagttaaaa ggtaggttta ggccgtcatg
     5581 aaaattacat cttaagtacg cccaactata gggcaaagtg aacaaagtcc tttgtgactt
     5641 ttagttttta ctgtcctggc agacatgctt aagagtaaat catgaaccat aaaccgattc
     5701 aatttaaaga cctcagtctt atctatccgc ataaaacctg ttttcaagac tttagtggtg
     5761 aaatccattt tgctgatcgc atcgcactga ttggacgtaa tggctcagga aaatcaacac
     5821 tactcaagat gctttgtggt ctttttttag cccctgcggg cgaaattaaa ataccccatg
     5881 atgttcgctt tggtcattta ccccaagtgc ttgaggattg tcctgattta agtggcggtc
     5941 agcgattaaa ccgagtatta agcaaaatac tgtcggatga gcctaatgtt ttgttgttag
     6001 atgagcccac aaatcatctg gacaggcgta atcgtcgttc tttaatgcgc atgcttgaac
     6061 attatcctgg gacattgatc atagcctctc atgatacaga actcatcaat acagttacag
     6121 atactctatg gcatattgat ttaggtcaag tgaccgtatt cagaggaatt tattttgatt
     6181 atcaacaaat tcttgaagac aagaaagcat cgattgaaca ggagttatca aggattgcaa
     6241 gacagaaaaa agaatcacat cttgctttga tgaaagagca ggagcgtaat aaacgtgcgc
     6301 gtgtccaggg tgaaaagaag atagcccaac gcaaatggcc aacgattcga tctcatacaa
     6361 agctcggttc agcaattaca acaggcaata aacgattaag tcacattaac tataaaaaac
     6421 aacaattact cgaggagctt tcctctctaa actttcagga ggtaattaag cccaaatttt
     6481 ggttaaaggg acttgggcac cataaaccct taatcaggat tcaagacgct tctgctgcct
     6541 atgaggctgt tgtgattctt gaagatattc attttcaact tagcggttgc gaacgagtgg
     6601 cattacatgg caacaatgct tcaggtaagt cgacctttgt caaagcgatc ttgggggaaa
     6661 gtcaaattaa gagaacaggg gaatgggctg tgcctgattg caatacaata ggctatatcg
     6721 atcaacatta tcaacatttg aacttcaatg aaacagtcct ggatttaata cgctccagta
     6781 tgccccatgc atctcataca gaaattcgtg ctcatttaaa tgatttttta tttcgcaaaa
     6841 atgaagaagt agaaattaag gtcaaaaatc tttctggtgg tgaaaaggca aggttatcga
     6901 tggcattgat tgctgcaaat ccacctaaac taattatttt agatgagatc acgaacaatg
     6961 tagatttgga aacacgcact catatcattc aggtgctgcg tgaatttcaa ggtgcaatgc
     7021 tggttatttc tcatgactat gactttttag aatccattca cattgaaacc aggtatcaga
     7081 ttcatcaagg aaagatacat catttcaatg agaaacaatt agaggtagtg catcatgact
     7141 aatcgtttgc ctaatcactt aggctctttt atgtggcatt ttttaaaacc atatcaaggc
     7201 atagtgatat tatttatcct gtttgcccta ttggccgggt tttgggggcc atttaatagc
     7261 ttattgatta agtctttcat taatactttg gctgaaaaaa caagtcagga cctatcttct
     7321 ttatattgga ttgctggatt attggtattg aactttattg tttttgacaa tataacctgg
     7381 cgaacacttg ggtacttgaa ctataaatat gaagcggtca ttaaaaacca aatcattagc
     7441 caaacatttg agtatgtgtt aggaggaagc acgcaatttt ttcaggataa tctatccggg
     7501 cgaattgccg atcaaatcac gacccttgct gataatcttg aggttatttt gcatcgtgtg
     7561 tctgtagatt ttcttcgtgg cgcttcatta ttagtggttt catttattac agcctatttt
     7621 gttaatgtct tgttctttta tattttgttt ctctggttcg tcgcctttgc ctcatttagt
     7681 atttggatgt cagcgcgatt agtccagttg tctgatgatc acgcaagttc tgaatcacag
     7741 ctttccgggc agttggtaga tagcctggcg aatcaatcca acattcgtat cttttcaaga
     7801 aaaatctacg aagtgaaacg tatgaacact ttctttcgct tggtacaaca ggcttttcaa
     7861 agaaaagagc tttttattgt tttgttatgt tgtgcacaag gaggaatgat tgcagtcatg
     7921 atgggtttgg catcgattac tttaatctat ttgtatggta aaggtcttgt gagcattggt
     7981 gattttgcct tgattctcgg actatccatg gaattgggtc atatgatgtg gtacaccatg
     8041 taccaagtgg atcaatttaa ccaagcctta ggcaaagcaa gacaaagttt gaatgcattg
     8101 gtgatacctc atgacattaa agataaaagt aacgcatctc aattagtagt tacccaagga
     8161 cgaattgaat tttccaaggt aaaatttcat taccaaggcg gttattctct gttccaaaat
     8221 aagtctgtaa cgattgaatc aggtcaaaaa gtgggtttag taggctattc aggcagtggt
     8281 aaatcgactt ttgtgaatct gattttacga ctttatgagg ttgtggatgg ccagattctc
     8341 attgatggtc aaaaccttgc cgaagtcacg caagaaagtt taaggcaagc tattgccatg
     8401 attccgcaag acccaactct ttttcatcga agcctaatgg acaatattcg ttatggcagg
     8461 acagatgctt ctgatgagga ggtcatttca gcttccaaga aagcccatgc acatgaattc
     8521 ataagtcttt tgccagaagg ctataaaacc ctggtagggg agcatggggt taaactttca
     8581 ggtggtcagc gccagcgcat tgcgattgcc cgagccatct taaaaaatgc tccgatttta
     8641 atgctggatg aggctacgtc tcagcttgat tccattacag aatccaatat tcaagagagc
     8701 ctctgggatt taatgcaggg aaaaacaacc ctggtagttg ctcatcgctt gtctaccctt
     8761 ctgcatatgg accgaatttt agtattcgat aaaggccata ttatcgaaga cggtacgcac
     8821 actgaactat tgaatcgtgg cggtttgtac aaaaccttgt gggatgccca agttggtggt
     8881 tttttgccag atcaacagaa tgaaaaagga gaataagcaa tgtcagtcaa gcaagagcag
     8941 tgtctcatta aagtggtttc ttatgattcg aattggcctg tgcaatttga acaggaagca
     9001 gagcgtatta aaaaagtgtt aggcagtaat tgcattgaaa ttcatcatat tggttccact
     9061 tcagtacctg atttggttgc aaaaccagtc attgatatga ttccagttgt tttagatatt
     9121 agcaaagtag atggagccaa tgctgccatg cgagcacttg gctatgaagc aaaaggagaa
     9181 tacggtattc cctttcgccg ttatttccaa aagggggata atcgacgaac gcatcatgct
     9241 catatttttg aacttggaaa ctctgaaata gatcggcatt taaagtttag ggattggatg
     9301 agaatgcacc cgaaagatag agaagcttat gcacgtttaa aacaagagtt ggcgcgccaa
     9361 tattctgatg acattaccgc ctattgcctg ggtaaagaag attttattgc aacaattgat
     9421 aaaaaagccg ggtttaatgg attaagaatg gttaaggcac taacaacacg tgaatgggat
     9481 aaggtgcgtc atttcagaca attttatttt tttggcaaag cagggctttc tgatccttat
     9541 acctggactt ttgagcataa tgcgcatgtt cattttgttt tatttcaagg aagtgaaatc
     9601 acaggttatg ctcatctgca attatggtct cataacagag cagcactacg gattatcgtt
     9661 atcgatgagc ttaaaagaaa ccatcaatat ggtacccgtt ttttggcatt atgtgaaaaa
     9721 tggctcaaaa gtcagggcta tcaaagtttg catgtcgagt cgtcaccaga tgctctgaaa
     9781 ttttatcgaa ataatggtta tatcgatatg ccttttgatg atcccgatgg ctatgaaggt
     9841 gatccacggg atactgcagt aggaaaaata ttatgattca aatagaaatt gtcattaaaa
     9901 cactggagtt atccgacatc cctcttctgg ttgatgcatt tcagaaagca aattgggaaa
     9961 aacccgcttc attgtttgaa gcctattacc aagaacagcg aaagtctctt cggatagtat
    10021 ggttggctta ttctcaagac caaattgctg gttacgttac tctaaaatgg atatcgcaat
    10081 accagccgtt cgcccatcaa aaaattccag aaattatgga tttaaatgta ttgccctctt
    10141 ttcgtaaaca gggagtgggg actgctttac tgcaagccgc tgaagaaaaa gcagcaggcg
    10201 aaagtgatgt cgtggggatt ggcgttggtt tatatggcgg ccatgatgga ggatacggac
    10261 aagctcaacg gctttatgtc aatcggggtt atatacctga tggtttaggt gtcacttatg
    10321 gttataagtc aactgtccca gggcagagct atcctttaga tgatgatttc attttatggt
    10381 ttacaaaaaa attaagatga aatgcttgac ccttacgtta cgtgagcact tatctttaaa
    10441 tggaatcatt tgagaggaca gtttaatggc ttatacggta aaacaattag caaaaatatc
    10501 gaatgtaagt gtgcgcactt tacattggta tgatgaaata ggcttattaa aaccagccta
    10561 tcaaggtgct aatggctatc gttattatga agaaaagcag tgcttaaaat tacagcaaat
    10621 tttattcttc agagagctgg gatttccctt gaatgatatt caaaaactgc ttatgcaaga
    10681 tgattttgac catatcaaag cattgagagc gcataaacag gttttggaaa gcgatatcaa
    10741 tcgaaaaaat caattgattg taacgattga taaaaccatt caacatttaa gaggtaagca
    10801 taagatgtct aatgaagaat tatattatgg ttttgattca ctgcgacaaa aacaatacga
    10861 aaaggaatta gttaaagaag gggtggtcag ccaggattgg atgaataact acaagaaaaa
    10921 aacaagtcaa tggactcagg ctgataaaga ccgatttatt caggagggta aagatatcaa
    10981 tcaagcactg atttcggcta ttaataatca acttaagcct gcctctccag aagtacaagc
    11041 cattatccat aggcaccatg cttgggtagg ttggaaccca agcaaggaag ggtatattgg
    11101 gttaagccaa agatatttga ctcctgagtt tagaaagttc tatgagtggc agcatcctga
    11161 tctgcttgaa ttcattattg aagggatgct gttttatgca aacaagaacc ttgattaagc
    11221 tgagagaagg gctgtgtctt ataagcccta aatgatattg gattgagagc attgtgcgtg
    11281 ttagcagaaa taatactgat ttatacatgc cggcttatca tgttgttcca aaccaggagc
    11341 ttttgtgagt tttttgctta ataattgatc tcttggtagg ttagaaaaat aatgactcaa
    11401 gaaattttaa aaactgcttg accctattgt tgctataggc cttacctttg tcttgaaggg
    11461 ggaaacacaa tgacacaatg gttcgtaaaa gatttaagcc aattaacagg tgtatcagtg
    11521 caaacgttac atcactatga tcgtatagga ttgctaaagc cttcattacg acttgccaat
    11581 gggtaccgtg tttactctga gaaggattta ttgaaattac aacagattat tgctttgaag
    11641 tttttcggtt ttgagttgtc acaaatcaaa accttactcg ctgaagaagg taatgcgctc
    11701 aatcatttta acagccaagc tcgggtgtta gaacaaaaag catccacttt acttgaggga
    11761 gctaaaactt tgagacgcat cattgactct gttgacaaaa atcaatctat cccttgggaa
    11821 accattattc aacttataga ggtatataaa atgacagaac atcttgagca tggctgggta
    11881 aaggaaatat tcacgccgga tgaattaaaa cagtacgctg agtttgaaaa ggaaatgaaa
    11941 gccaatgcca cgcctgagca aaaagcggct atggaaaaca attggtctca gttagttgat
    12001 gaggtaaaaa ataatctcaa gcaagatcca gactcaaaaa tagtaattta tttaggaaag
    12061 aagattatgg attgggtaaa tggggtctat ggtaaaaaat atgcccattt aaggaccaaa
    12121 aaatttgaac agggttttgg ggaaggaaaa ggtcttgatg aggttggatt aacaccggaa
    12181 atcgtatcct ggatggataa agcgatggat gcttactgga gagaccgaat ctatgggatt
    12241 cttgatcaag taggaaaagc tccttcagca agcatattag atctttggaa tgaaatgctg
    12301 gttgatatgt atggtgagga aacttcacga aaaaatgcca tatatgaaat cgctttaaat
    12361 gacgatcagg ttagccctga ggcaaa
//
LOCUS       DASLOE010000404         2091 bp    DNA     linear   BCT 18-DEC-2023
DEFINITION  TPA_asm: Legionella pneumophila isolate LP000402
            SAMN38858284-rid20595953.guided.283, whole genome shotgun sequence.
ACCESSION   DASLOE010000404 DASLOE010000000
VERSION     DASLOE010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38858284
            Sequence Read Archive: SRR27225877
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Legionella pneumophila
  ORGANISM  Legionella pneumophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Legionellales;
            Legionellaceae; Legionella.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002031237.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002031237.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/16/2023 05:07:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 3,029
            CDSs (with protein)               :: 3,029
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 12 of 38
            Pseudo Genes (incomplete)         :: 23 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 5 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Legionella pneumophila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38858284-rid20595953.guided.283"
                     /isolate="LP000402"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:446"
                     /country="USA: New York"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2017-08"
                     /collected_by="NYCDOHMH Public Health Lab"
     gene            141..1139
                     /gene="aph(9)-Ia"
                     /locus_tag="U6309_003113"
     CDS             141..1139
                     /gene="aph(9)-Ia"
                     /locus_tag="U6309_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(9)-Ia"
                     /protein_id="HEN5506500.1"
                     /translation="MLRNNIPDQHLIALLKVYYGIDIHTVQLIVGGADMNAFGYKADS
                     ESNSYFVKLKYGHHDEINLSIIRLLHDSGVKEIVFPINTLEANLFQQVDHVKIIVYPF
                     IDAPNGFTKNLTEKQWKQLGKILRQIHGTSVPTSIQQRLRKETYSPKWREIVRSFYSQ
                     LGFDERDDQITTDFKAFFNQNIDSIHQLVNSSGELSKKIQPDFEKYVLCHSDVHAGNV
                     LVVDEESIYIIDWDEPMFAPKERDLMFIGSGIGNVWNKPHEINYFYEGYGKTSIDKTI
                     LSYYRHERIVEDIAVYGHDLLSRDQNAQSRLESFKHFKSMFTPNDVVEIAFATGLI"
     gene            complement(1328..>2091)
                     /locus_tag="U6309_003114"
     CDS             complement(1328..>2091)
                     /locus_tag="U6309_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN5506501.1"
                     /translation="ERSISTIIARLKNAVDLKEEFFKTDKDYVVWIENVISARKNVPI
                     ELAKIKEQLLKMKEGEFDYYVKDLSTNEYNGGISINRRLFRDLGLKGEFFKTQKDYNA
                     WIEDVISARKFNNFSTNIDDILNKFEEKIKNIDTNYPEAKIKANELLISLRKNKDEAF
                     SNPSLESLYDFADKSKQMIKSTISSLKRESGMEVFLSDLAEQILNTINTFLNNTLNSS
                     ASNRSGFFGFKSSYEKVIAQELEKNIDKELKDFKP"
BASE COUNT          670 a          352 c          318 g          751 t
ORIGIN      
        1 gtgatagggc cacctctcaa tttactcatt aagtctgatt attttgataa tcagacttaa
       61 tgtgattata gtaataatag tattctaatt atattatttg ctttaccatt atgataattt
      121 ccaaatagta attaactctt atgctaagaa ataacattcc agatcaacac ctcattgcgc
      181 ttttgaaagt ctattatgga attgatattc atacagtaca actcatcgtg ggtggtgctg
      241 atatgaatgc ctttggatat aaagcagatt cagaatccaa ttcttatttc gtaaagctaa
      301 aatacggcca tcatgatgaa attaatttat caatcatccg tctcttacat gactctggag
      361 taaaggaaat tgtttttcct atcaacacac tggaagcaaa cttatttcag caagtggatc
      421 atgttaaaat aattgtgtat ccatttattg atgcgcccaa tggcttcacc aaaaatttaa
      481 cagaaaaaca gtggaaacag cttgggaaaa tattaagaca aatccatgga acctctgttc
      541 ccacctcgat tcaacagcga ttaagaaaag aaacttactc acctaaatgg cgtgaaatcg
      601 ttaggtcttt ttacagtcaa cttggatttg atgagcgtga tgatcaaata accactgact
      661 tcaaagcttt ttttaatcaa aatatcgatt caatccatca attagttaat tcttcaggag
      721 aactgtctaa aaaaattcaa cctgatttcg aaaaatatgt actttgtcat tcagacgtac
      781 atgcaggtaa tgtgttggtc gttgatgagg aatctattta cattattgat tgggatgagc
      841 ccatgttcgc accaaaagaa cgtgatttga tgttcattgg cagtggtatt ggtaatgtat
      901 ggaataaacc tcatgaaatc aattattttt atgaaggtta tggtaaaaca agcatcgata
      961 aaaccatttt gtcttattac agacatgagc gaattgttga agatatagcc gtgtatggac
     1021 atgacttgct ttcacgtgat caaaatgctc aatccagact tgaaagtttt aaacatttta
     1081 aatcgatgtt tactccaaat gatgtagttg aaatagcttt tgctacaggt ttgatttgat
     1141 ttggataaac acagaagaaa aaagatgaac cgtttaatct caaccctcct ctttttaatt
     1201 tcgtataaac tttgaattgc attaaatcac ctgaaatttt ttatataacg catcccaact
     1261 ttagtaagaa gacgacatga ctgtttgtgt tatcgcaatg ataagctctt gtaaataatt
     1321 ttaaatttta tggtttaaaa tcttttaatt ccttgtcgat attcttttca agttcctggg
     1381 ctattacttt ttcataagac gacttaaaac caaaaaaacc actacgattt gaggcacttg
     1441 agttcaatgt attatttaga aatgtattta tagtatttag aatttgctca gcgagatcac
     1501 ttaaaaaaac ttccattcct gactctctct ttaaagatga aatggtactt tttatcattt
     1561 gtttcgattt atctgcaaag tcatagagcg actctaggga aggatttgaa aatgcttcgt
     1621 ctttattttt tctaagcgat atcaacaact cattggcttt gatttttgcc tctggataat
     1681 tagtatctat attttttatt ttttcttcaa atttatttaa aatatcatca atattggtac
     1741 taaaattatt aaattttctt gcagaaatta cgtcttctat ccatgcgtta taatcttttt
     1801 gtgtcttgaa aaactccccc ttaagaccta aatctcgaaa aagtcgtcgg ttaatagata
     1861 tgccgccatt gtattcattg gtacttaaat ctttaacata ataatcaaac tcaccttctt
     1921 tcattttcaa taattgttct tttatctttg ctaactctat gggaacattt tttcttgcag
     1981 aaattacgtt ttctatccag acaacataat ctttgtctgt cttgaaaaac tcttccttaa
     2041 gatctactgc attcttgagc cgcgcaatga ttgtactaat gctgcgttct g
//
